LOCUS       DBBGLQ010000001       143945 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.001, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000001 DBBGLQ010000000
VERSION     DBBGLQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 143945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 143945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..143945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.001"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            644..994
                     /gene="flhD"
                     /locus_tag="ACIYSB_000001"
     CDS             644..994
                     /gene="flhD"
                     /locus_tag="ACIYSB_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGC5427136.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDALQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            997..1575
                     /gene="flhC"
                     /locus_tag="ACIYSB_000002"
     CDS             997..1575
                     /gene="flhC"
                     /locus_tag="ACIYSB_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGC5427137.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            1702..2589
                     /gene="motA"
                     /locus_tag="ACIYSB_000003"
     CDS             1702..2589
                     /gene="motA"
                     /locus_tag="ACIYSB_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGC5427138.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            2586..3512
                     /gene="motB"
                     /locus_tag="ACIYSB_000004"
     CDS             2586..3512
                     /gene="motB"
                     /locus_tag="ACIYSB_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGC5427139.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            3517..5481
                     /gene="cheA"
                     /locus_tag="ACIYSB_000005"
     CDS             3517..5481
                     /gene="cheA"
                     /locus_tag="ACIYSB_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGC5427140.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            5502..6005
                     /gene="cheW"
                     /locus_tag="ACIYSB_000006"
     CDS             5502..6005
                     /gene="cheW"
                     /locus_tag="ACIYSB_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGC5427141.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            6150..7811
                     /gene="tar"
                     /locus_tag="ACIYSB_000007"
     CDS             6150..7811
                     /gene="tar"
                     /locus_tag="ACIYSB_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGC5427142.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            7857..9458
                     /gene="tap"
                     /locus_tag="ACIYSB_000008"
     CDS             7857..9458
                     /gene="tap"
                     /locus_tag="ACIYSB_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGC5427143.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRGLQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESVQLQIAPVVS"
     gene            9477..10337
                     /gene="cheR"
                     /locus_tag="ACIYSB_000009"
     CDS             9477..10337
                     /gene="cheR"
                     /locus_tag="ACIYSB_000009"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGC5427144.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            10340..11389
                     /gene="cheB"
                     /locus_tag="ACIYSB_000010"
     CDS             10340..11389
                     /gene="cheB"
                     /locus_tag="ACIYSB_000010"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGC5427145.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYNEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            11404..11793
                     /gene="cheY"
                     /locus_tag="ACIYSB_000011"
     CDS             11404..11793
                     /gene="cheY"
                     /locus_tag="ACIYSB_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGC5427146.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVIFDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            11804..12448
                     /gene="cheZ"
                     /locus_tag="ACIYSB_000012"
     CDS             11804..12448
                     /gene="cheZ"
                     /locus_tag="ACIYSB_000012"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGC5427147.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            12650..13798
                     /gene="flhB"
                     /locus_tag="ACIYSB_000013"
     CDS             12650..13798
                     /gene="flhB"
                     /locus_tag="ACIYSB_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGC5427148.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            13791..15869
                     /gene="flhA"
                     /locus_tag="ACIYSB_000014"
     CDS             13791..15869
                     /gene="flhA"
                     /locus_tag="ACIYSB_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGC5427149.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NIVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            15869..16261
                     /gene="flhe"
                     /locus_tag="ACIYSB_000015"
     CDS             15869..16261
                     /gene="flhe"
                     /locus_tag="ACIYSB_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGC5427150.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(16381..16869)
                     /gene="yecT"
                     /locus_tag="ACIYSB_000016"
     CDS             complement(16381..16869)
                     /gene="yecT"
                     /locus_tag="ACIYSB_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGC5427151.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DVNVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSAPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            16960..17004
                     /gene="yecV"
                     /locus_tag="ACIYSB_000017"
     CDS             16960..17004
                     /gene="yecV"
                     /locus_tag="ACIYSB_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HGC5427152.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            complement(17046..18779)
                     /gene="argS"
                     /locus_tag="ACIYSB_000018"
     CDS             complement(17046..18779)
                     /gene="argS"
                     /locus_tag="ACIYSB_000018"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGC5427153.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            18995..19561
                     /gene="yecM"
                     /locus_tag="ACIYSB_000019"
     CDS             18995..19561
                     /gene="yecM"
                     /locus_tag="ACIYSB_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGC5427154.1"
                     /translation="MANWQSIDELQDIASDLPRFIHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            19575..20321
                     /gene="cutC"
                     /locus_tag="ACIYSB_000020"
     CDS             19575..20321
                     /gene="cutC"
                     /locus_tag="ACIYSB_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGC5427155.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            20709..21809
                     /gene="torY"
                     /locus_tag="ACIYSB_000021"
     CDS             20709..21809
                     /gene="torY"
                     /locus_tag="ACIYSB_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGC5427156.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDSET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSHIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            21834..24263
                     /gene="torZ"
                     /locus_tag="ACIYSB_000022"
     CDS             21834..24263
                     /gene="torZ"
                     /locus_tag="ACIYSB_000022"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGC5427157.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKSNGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFDDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGHASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAIADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(24428..25399)
                     /gene="cmoB"
                     /locus_tag="ACIYSB_000023"
     CDS             complement(24428..25399)
                     /gene="cmoB"
                     /locus_tag="ACIYSB_000023"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGC5427158.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(25396..26139)
                     /gene="cmoA"
                     /locus_tag="ACIYSB_000024"
     CDS             complement(25396..26139)
                     /gene="cmoA"
                     /locus_tag="ACIYSB_000024"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGC5427159.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HNAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(26180..26575)
                     /gene="yecN"
                     /locus_tag="ACIYSB_000025"
     CDS             complement(26180..26575)
                     /gene="yecN"
                     /locus_tag="ACIYSB_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGC5427160.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(26628..27446)
                     /gene="yecE"
                     /locus_tag="ACIYSB_000026"
     CDS             complement(26628..27446)
                     /gene="yecE"
                     /locus_tag="ACIYSB_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGC5427161.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            complement(27443..28009)
                     /gene="yecD"
                     /locus_tag="ACIYSB_000027"
     CDS             complement(27443..28009)
                     /gene="yecD"
                     /locus_tag="ACIYSB_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGC5427162.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            28319..30091
                     /gene="aspS"
                     /locus_tag="ACIYSB_000028"
     CDS             28319..30091
                     /gene="aspS"
                     /locus_tag="ACIYSB_000028"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGC5427163.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            30209..30661
                     /gene="nudB"
                     /locus_tag="ACIYSB_000029"
     CDS             30209..30661
                     /gene="nudB"
                     /locus_tag="ACIYSB_000029"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGC5427164.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            30690..31430
                     /gene="yebC"
                     /locus_tag="ACIYSB_000030"
     CDS             30690..31430
                     /gene="yebC"
                     /locus_tag="ACIYSB_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGC5427165.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            31465..31986
                     /gene="ruvC"
                     /locus_tag="ACIYSB_000031"
     CDS             31465..31986
                     /gene="ruvC"
                     /locus_tag="ACIYSB_000031"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGC5427166.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(31988..32590)
                     /gene="yebB"
                     /locus_tag="ACIYSB_000032"
     CDS             complement(31988..32590)
                     /gene="yebB"
                     /locus_tag="ACIYSB_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGC5427167.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTERQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            32661..32726
                     /gene="yobI"
                     /locus_tag="ACIYSB_000033"
     CDS             32661..32726
                     /gene="yobI"
                     /locus_tag="ACIYSB_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HGC5427168.1"
                     /translation="MYIFITHFFTEYVILKYLLPI"
     gene            32865..33476
                     /gene="ruvA"
                     /locus_tag="ACIYSB_000034"
     CDS             32865..33476
                     /gene="ruvA"
                     /locus_tag="ACIYSB_000034"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGC5427169.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            33485..34495
                     /gene="ruvB"
                     /locus_tag="ACIYSB_000035"
     CDS             33485..34495
                     /gene="ruvB"
                     /locus_tag="ACIYSB_000035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGC5427170.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(34642..35427)
                     /gene="znuB"
                     /locus_tag="ACIYSB_000036"
     CDS             complement(34642..35427)
                     /gene="znuB"
                     /locus_tag="ACIYSB_000036"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGC5427171.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAVYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(35424..36179)
                     /gene="znuC"
                     /locus_tag="ACIYSB_000037"
     CDS             complement(35424..36179)
                     /gene="znuC"
                     /locus_tag="ACIYSB_000037"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGC5427172.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            36258..37190
                     /gene="znuA"
                     /locus_tag="ACIYSB_000038"
     CDS             36258..37190
                     /gene="znuA"
                     /locus_tag="ACIYSB_000038"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGC5427173.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            37206..38528
                     /gene="mepM"
                     /locus_tag="ACIYSB_000039"
     CDS             37206..38528
                     /gene="mepM"
                     /locus_tag="ACIYSB_000039"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGC5427174.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            38648..39619
                     /gene="lpxM"
                     /locus_tag="ACIYSB_000040"
                     /gene_synonym="msbB"
     CDS             38648..39619
                     /gene="lpxM"
                     /locus_tag="ACIYSB_000040"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGC5427175.1"
                     /translation="METKKNNSEHIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(39750..41192)
                     /gene="pyk"
                     /locus_tag="ACIYSB_000041"
     CDS             complement(39750..41192)
                     /gene="pyk"
                     /locus_tag="ACIYSB_000041"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGC5427176.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(41320..42189)
                     /gene="hexR"
                     /locus_tag="ACIYSB_000042"
                     /gene_synonym="yebK"
     CDS             complement(41320..42189)
                     /gene="hexR"
                     /locus_tag="ACIYSB_000042"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGC5427177.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            42527..44002
                     /gene="zwf"
                     /locus_tag="ACIYSB_000043"
     CDS             42527..44002
                     /gene="zwf"
                     /locus_tag="ACIYSB_000043"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGC5427178.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            44237..46048
                     /gene="edd"
                     /locus_tag="ACIYSB_000044"
     CDS             44237..46048
                     /gene="edd"
                     /locus_tag="ACIYSB_000044"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGC5427179.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            46085..46726
                     /gene="kdgA"
                     /locus_tag="ACIYSB_000045"
                     /gene_synonym="eda"
     CDS             46085..46726
                     /gene="kdgA"
                     /locus_tag="ACIYSB_000045"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGC5427180.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(46782..47960)
                     /gene="purT"
                     /locus_tag="ACIYSB_000046"
     CDS             complement(46782..47960)
                     /gene="purT"
                     /locus_tag="ACIYSB_000046"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGC5427181.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            48094..48384
                     /gene="yebG"
                     /locus_tag="ACIYSB_000047"
     CDS             48094..48384
                     /gene="yebG"
                     /locus_tag="ACIYSB_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGC5427182.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            48451..48807
                     /gene="yebF"
                     /locus_tag="ACIYSB_000048"
     CDS             48451..48807
                     /gene="yebF"
                     /locus_tag="ACIYSB_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGC5427183.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            49134..49793
                     /gene="yebE"
                     /locus_tag="ACIYSB_000049"
     CDS             49134..49793
                     /gene="yebE"
                     /locus_tag="ACIYSB_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGC5427184.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLVKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ARLILALVFAAKSDGHIDAKERAAIDQQLRGAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            50002..52062
                     /gene="ptrB"
                     /locus_tag="ACIYSB_000050"
     CDS             50002..52062
                     /gene="ptrB"
                     /locus_tag="ACIYSB_000050"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGC5427185.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHARGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQEPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(52059..52721)
                     /gene="exoX"
                     /locus_tag="ACIYSB_000051"
     CDS             complement(52059..52721)
                     /gene="exoX"
                     /locus_tag="ACIYSB_000051"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGC5427186.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(52745..53401)
                     /gene="yobB"
                     /locus_tag="ACIYSB_000052"
     CDS             complement(52745..53401)
                     /gene="yobB"
                     /locus_tag="ACIYSB_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGC5427187.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMNIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(53503..53733)
                     /gene="holE"
                     /locus_tag="ACIYSB_000053"
     CDS             complement(53503..53733)
                     /gene="holE"
                     /locus_tag="ACIYSB_000053"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGC5427188.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            53872..54246
                     /gene="yobA"
                     /locus_tag="ACIYSB_000054"
     CDS             53872..54246
                     /gene="yobA"
                     /locus_tag="ACIYSB_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGC5427189.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            54250..55122
                     /gene="copD"
                     /locus_tag="ACIYSB_000055"
     CDS             54250..55122
                     /gene="copD"
                     /locus_tag="ACIYSB_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGC5427190.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGIWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            55135..55476
                     /gene="yebY"
                     /locus_tag="ACIYSB_000056"
     CDS             55135..55476
                     /gene="yebY"
                     /locus_tag="ACIYSB_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGC5427191.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            55872..56528
                     /gene="pphA"
                     /locus_tag="ACIYSB_000057"
     CDS             55872..56528
                     /gene="pphA"
                     /locus_tag="ACIYSB_000057"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGC5427192.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLCRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(56529..56720)
                     /gene="yebW"
                     /locus_tag="ACIYSB_000058"
     CDS             complement(56529..56720)
                     /gene="yebW"
                     /locus_tag="ACIYSB_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGC5427193.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(56825..57061)
                     /gene="yebV"
                     /locus_tag="ACIYSB_000059"
     CDS             complement(56825..57061)
                     /gene="yebV"
                     /locus_tag="ACIYSB_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGC5427194.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(57179..58618)
                     /gene="rsmF"
                     /locus_tag="ACIYSB_000060"
     CDS             complement(57179..58618)
                     /gene="rsmF"
                     /locus_tag="ACIYSB_000060"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGC5427195.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAATGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(58698..61331)
                     /gene="yebT"
                     /locus_tag="ACIYSB_000061"
     CDS             complement(58698..61331)
                     /gene="yebT"
                     /locus_tag="ACIYSB_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGC5427196.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIASWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(61300..62583)
                     /gene="yebS"
                     /locus_tag="ACIYSB_000062"
     CDS             complement(61300..62583)
                     /gene="yebS"
                     /locus_tag="ACIYSB_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGC5427197.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            62713..63210
                     /gene="msrC"
                     /locus_tag="ACIYSB_000063"
     CDS             62713..63210
                     /gene="msrC"
                     /locus_tag="ACIYSB_000063"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGC5427198.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDINW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            63307..64005
                     /gene="proQ"
                     /locus_tag="ACIYSB_000064"
     CDS             63307..64005
                     /gene="proQ"
                     /locus_tag="ACIYSB_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGC5427199.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAATAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            64025..66073
                     /gene="prc"
                     /locus_tag="ACIYSB_000065"
     CDS             64025..66073
                     /gene="prc"
                     /locus_tag="ACIYSB_000065"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGC5427200.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            66265..67146
                     /gene="htpX"
                     /locus_tag="ACIYSB_000066"
     CDS             66265..67146
                     /gene="htpX"
                     /locus_tag="ACIYSB_000066"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGC5427201.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMALCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(67192..68565)
                     /gene="yebQ"
                     /locus_tag="ACIYSB_000067"
     CDS             complement(67192..68565)
                     /gene="yebQ"
                     /locus_tag="ACIYSB_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5427202.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            68742..69533
                     /gene="kdgR"
                     /locus_tag="ACIYSB_000068"
     CDS             68742..69533
                     /gene="kdgR"
                     /locus_tag="ACIYSB_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGC5427203.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMRELSR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(69677..69916)
                     /gene="yobH"
                     /locus_tag="ACIYSB_000069"
     CDS             complement(69677..69916)
                     /gene="yobH"
                     /locus_tag="ACIYSB_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGC5427204.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            70075..70218
                     /gene="mgrB"
                     /locus_tag="ACIYSB_000070"
     CDS             70075..70218
                     /gene="mgrB"
                     /locus_tag="ACIYSB_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGC5427205.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            70293..70580
                     /gene="yebO"
                     /locus_tag="ACIYSB_000071"
     CDS             70293..70580
                     /gene="yebO"
                     /locus_tag="ACIYSB_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGC5427206.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            71250..71393
                     /gene="yobF"
                     /locus_tag="ACIYSB_000072"
     CDS             71250..71393
                     /gene="yobF"
                     /locus_tag="ACIYSB_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGC5427207.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            71406..71615
                     /gene="cspE"
                     /locus_tag="ACIYSB_000073"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             71406..71615
                     /gene="cspE"
                     /locus_tag="ACIYSB_000073"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGC5427208.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            71781..72590
                     /gene="rlmA"
                     /locus_tag="ACIYSB_000074"
     CDS             71781..72590
                     /gene="rlmA"
                     /locus_tag="ACIYSB_000074"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGC5427209.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(72587..73153)
                     /gene="mntP"
                     /locus_tag="ACIYSB_000075"
     CDS             complement(72587..73153)
                     /gene="mntP"
                     /locus_tag="ACIYSB_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGC5427210.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(73582..74040)
                     /gene="yobD"
                     /locus_tag="ACIYSB_000076"
     CDS             complement(73582..74040)
                     /gene="yobD"
                     /locus_tag="ACIYSB_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGC5427211.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(74095..74946)
                     /gene="manZ"
                     /locus_tag="ACIYSB_000077"
     CDS             complement(74095..74946)
                     /gene="manZ"
                     /locus_tag="ACIYSB_000077"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGC5427212.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(74959..75759)
                     /gene="manY"
                     /locus_tag="ACIYSB_000078"
     CDS             complement(74959..75759)
                     /gene="manY"
                     /locus_tag="ACIYSB_000078"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGC5427213.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(75822..76793)
                     /gene="manX"
                     /locus_tag="ACIYSB_000079"
     CDS             complement(75822..76793)
                     /gene="manX"
                     /locus_tag="ACIYSB_000079"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGC5427214.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            77110..77268
                     /gene="yoaL"
                     /locus_tag="ACIYSB_000080"
     CDS             77110..77268
                     /gene="yoaL"
                     /locus_tag="ACIYSB_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGC5427215.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            77256..78812
                     /gene="yoaE"
                     /locus_tag="ACIYSB_000081"
     CDS             77256..78812
                     /gene="yoaE"
                     /locus_tag="ACIYSB_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGC5427216.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(78816..80414)
                     /gene="pdeD"
                     /locus_tag="ACIYSB_000082"
                     /pseudo
     CDS             complement(78816..80414)
                     /gene="pdeD"
                     /locus_tag="ACIYSB_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(80545..81909)
                     /gene="sdaA"
                     /locus_tag="ACIYSB_000083"
     CDS             complement(80545..81909)
                     /gene="sdaA"
                     /locus_tag="ACIYSB_000083"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGC5427217.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(82093..82671)
                     /gene="nudL"
                     /locus_tag="ACIYSB_000084"
     CDS             complement(82093..82671)
                     /gene="nudL"
                     /locus_tag="ACIYSB_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGC5427218.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASAIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(82675..84036)
                     /gene="pabB"
                     /locus_tag="ACIYSB_000085"
     CDS             complement(82675..84036)
                     /gene="pabB"
                     /locus_tag="ACIYSB_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGC5427219.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAVKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNSWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIFCSAGGGIVADSQEEAEYQETFDKVNRILKQ
                     LEK"
     gene            84110..84289
                     /gene="yoaH"
                     /locus_tag="ACIYSB_000086"
     CDS             84110..84289
                     /gene="yoaH"
                     /locus_tag="ACIYSB_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGC5427220.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(84409..84708)
                     /gene="yoaC"
                     /locus_tag="ACIYSB_000087"
     CDS             complement(84409..84708)
                     /gene="yoaC"
                     /locus_tag="ACIYSB_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGC5427221.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(85130..85474)
                     /gene="yoaB"
                     /locus_tag="ACIYSB_000088"
     CDS             complement(85130..85474)
                     /gene="yoaB"
                     /locus_tag="ACIYSB_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGC5427222.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            85606..87516
                     /gene="yoaA"
                     /locus_tag="ACIYSB_000089"
     CDS             85606..87516
                     /gene="yoaA"
                     /locus_tag="ACIYSB_000089"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGC5427223.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            87574..88269
                     /gene="tsaB"
                     /locus_tag="ACIYSB_000090"
     CDS             87574..88269
                     /gene="tsaB"
                     /locus_tag="ACIYSB_000090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGC5427224.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            88309..88890
                     /gene="yeaY"
                     /locus_tag="ACIYSB_000091"
     CDS             88309..88890
                     /gene="yeaY"
                     /locus_tag="ACIYSB_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGC5427225.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            89095..90780
                     /gene="fadD"
                     /locus_tag="ACIYSB_000092"
     CDS             89095..90780
                     /gene="fadD"
                     /locus_tag="ACIYSB_000092"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGC5427226.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            90862..91977
                     /gene="rnd"
                     /locus_tag="ACIYSB_000093"
     CDS             90862..91977
                     /gene="rnd"
                     /locus_tag="ACIYSB_000093"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGC5427227.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(92031..92996)
                     /gene="yeaX"
                     /locus_tag="ACIYSB_000094"
     CDS             complement(92031..92996)
                     /gene="yeaX"
                     /locus_tag="ACIYSB_000094"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGC5427228.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(93052..94176)
                     /gene="yeaW"
                     /locus_tag="ACIYSB_000095"
     CDS             complement(93052..94176)
                     /gene="yeaW"
                     /locus_tag="ACIYSB_000095"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGC5427229.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDMFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(94208..95689)
                     /gene="yeaV"
                     /locus_tag="ACIYSB_000096"
                     /pseudo
     CDS             complement(94208..95689)
                     /gene="yeaV"
                     /locus_tag="ACIYSB_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
     gene            complement(95844..96929)
                     /gene="dmlA"
                     /locus_tag="ACIYSB_000097"
     CDS             complement(95844..96929)
                     /gene="dmlA"
                     /locus_tag="ACIYSB_000097"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGC5427230.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            97032..97955
                     /gene="dmlR"
                     /locus_tag="ACIYSB_000098"
     CDS             97032..97955
                     /gene="dmlR"
                     /locus_tag="ACIYSB_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGC5427231.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            98082..98720
                     /gene="leuE"
                     /locus_tag="ACIYSB_000099"
     CDS             98082..98720
                     /gene="leuE"
                     /locus_tag="ACIYSB_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGC5427232.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILAATL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            98893..99252
                     /gene="yeaR"
                     /locus_tag="ACIYSB_000100"
     CDS             98893..99252
                     /gene="yeaR"
                     /locus_tag="ACIYSB_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGC5427233.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            99256..99438
                     /gene="yoaG"
                     /locus_tag="ACIYSB_000101"
     CDS             99256..99438
                     /gene="yoaG"
                     /locus_tag="ACIYSB_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGC5427234.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            99586..99834
                     /gene="yeaQ"
                     /locus_tag="ACIYSB_000102"
     CDS             99586..99834
                     /gene="yeaQ"
                     /locus_tag="ACIYSB_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGC5427235.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(99893..99967)
                     /gene="yoaJ"
                     /locus_tag="ACIYSB_000103"
     CDS             complement(99893..99967)
                     /gene="yoaJ"
                     /locus_tag="ACIYSB_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGC5427236.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(99970..100068)
                     /gene="yoaK"
                     /locus_tag="ACIYSB_000104"
     CDS             complement(99970..100068)
                     /gene="yoaK"
                     /locus_tag="ACIYSB_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGC5427237.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(100101..101126)
                     /gene="dgcP"
                     /locus_tag="ACIYSB_000105"
     CDS             complement(100101..101126)
                     /gene="dgcP"
                     /locus_tag="ACIYSB_000105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGC5427238.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMYIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            101309..101563
                     /gene="yoaF"
                     /locus_tag="ACIYSB_000106"
     CDS             101309..101563
                     /gene="yoaF"
                     /locus_tag="ACIYSB_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGC5427239.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(101585..101932)
                     /gene="yeaO"
                     /locus_tag="ACIYSB_000107"
     CDS             complement(101585..101932)
                     /gene="yeaO"
                     /locus_tag="ACIYSB_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGC5427240.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDYATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(101987..103168)
                     /gene="nimT"
                     /locus_tag="ACIYSB_000108"
     CDS             complement(101987..103168)
                     /gene="nimT"
                     /locus_tag="ACIYSB_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGC5427241.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            103265..104086
                     /gene="nimR"
                     /locus_tag="ACIYSB_000109"
     CDS             103265..104086
                     /gene="nimR"
                     /locus_tag="ACIYSB_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGC5427242.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIARLTTVSPQSAKPDPRQ"
     gene            complement(104043..104489)
                     /gene="yeaL"
                     /locus_tag="ACIYSB_000110"
     CDS             complement(104043..104489)
                     /gene="yeaL"
                     /locus_tag="ACIYSB_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGC5427243.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            104659..104763
                     /gene="yoaI"
                     /locus_tag="ACIYSB_000111"
     CDS             104659..104763
                     /gene="yoaI"
                     /locus_tag="ACIYSB_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGC5427244.1"
                     /translation="MNDQMFVETMIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(104764..105267)
                     /gene="yeaK"
                     /locus_tag="ACIYSB_000112"
     CDS             complement(104764..105267)
                     /gene="yeaK"
                     /locus_tag="ACIYSB_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGC5427245.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TSLGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(105310..106800)
                     /gene="dgcJ"
                     /locus_tag="ACIYSB_000113"
     CDS             complement(105310..106800)
                     /gene="dgcJ"
                     /locus_tag="ACIYSB_000113"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGC5427246.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(106981..108456)
                     /gene="cdgI"
                     /locus_tag="ACIYSB_000114"
     CDS             complement(106981..108456)
                     /gene="cdgI"
                     /locus_tag="ACIYSB_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGC5427247.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            complement(108603..109886)
                     /gene="yeaH"
                     /locus_tag="ACIYSB_000115"
     CDS             complement(108603..109886)
                     /gene="yeaH"
                     /locus_tag="ACIYSB_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGC5427248.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKG"
     gene            complement(109999..111933)
                     /gene="yeaG"
                     /locus_tag="ACIYSB_000116"
     CDS             complement(109999..111933)
                     /gene="yeaG"
                     /locus_tag="ACIYSB_000116"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGC5427249.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            112369..113115
                     /gene="mipA"
                     /locus_tag="ACIYSB_000117"
     CDS             112369..113115
                     /gene="mipA"
                     /locus_tag="ACIYSB_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGC5427250.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            113205..114059
                     /gene="yeaE"
                     /locus_tag="ACIYSB_000118"
     CDS             113205..114059
                     /gene="yeaE"
                     /locus_tag="ACIYSB_000118"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGC5427251.1"
                     /translation="MQQKMIQFSGDVSLPAVGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALYMV"
     gene            complement(114107..114991)
                     /gene="yeaD"
                     /locus_tag="ACIYSB_000119"
     CDS             complement(114107..114991)
                     /gene="yeaD"
                     /locus_tag="ACIYSB_000119"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGC5427252.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKENVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(115075..116070)
                     /gene="gapA"
                     /locus_tag="ACIYSB_000120"
     CDS             complement(115075..116070)
                     /gene="gapA"
                     /locus_tag="ACIYSB_000120"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGC5427253.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            116412..116825
                     /gene="msrB"
                     /locus_tag="ACIYSB_000121"
     CDS             116412..116825
                     /gene="msrB"
                     /locus_tag="ACIYSB_000121"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGC5427254.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            116867..117139
                     /gene="yeaC"
                     /locus_tag="ACIYSB_000122"
     CDS             116867..117139
                     /gene="yeaC"
                     /locus_tag="ACIYSB_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGC5427255.1"
                     /translation="MNLDDIINSMMPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNIEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            117509..118585
                     /gene="ydjL"
                     /locus_tag="ACIYSB_000123"
     CDS             117509..118585
                     /gene="ydjL"
                     /locus_tag="ACIYSB_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGC5427256.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPMCGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            118612..119991
                     /gene="ydjK"
                     /locus_tag="ACIYSB_000124"
     CDS             118612..119991
                     /gene="ydjK"
                     /locus_tag="ACIYSB_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5427257.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            120008..121051
                     /gene="ydjJ"
                     /locus_tag="ACIYSB_000125"
     CDS             120008..121051
                     /gene="ydjJ"
                     /locus_tag="ACIYSB_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HGC5427258.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            121072..121908
                     /gene="ydjI"
                     /locus_tag="ACIYSB_000126"
     CDS             121072..121908
                     /gene="ydjI"
                     /locus_tag="ACIYSB_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGC5427259.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            121913..122860
                     /gene="ydjH"
                     /locus_tag="ACIYSB_000127"
     CDS             121913..122860
                     /gene="ydjH"
                     /locus_tag="ACIYSB_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGC5427260.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            122870..123850
                     /gene="ydjG"
                     /locus_tag="ACIYSB_000128"
     CDS             122870..123850
                     /gene="ydjG"
                     /locus_tag="ACIYSB_000128"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HGC5427261.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVAASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKSE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            123987..124745
                     /gene="ydjF"
                     /locus_tag="ACIYSB_000129"
     CDS             123987..124745
                     /gene="ydjF"
                     /locus_tag="ACIYSB_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGC5427262.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSGLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     IRRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            124862..126220
                     /gene="ydjE"
                     /locus_tag="ACIYSB_000130"
     CDS             124862..126220
                     /gene="ydjE"
                     /locus_tag="ACIYSB_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5427263.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVSNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAECQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(126313..126954)
                     /gene="pncA"
                     /locus_tag="ACIYSB_000131"
     CDS             complement(126313..126954)
                     /gene="pncA"
                     /locus_tag="ACIYSB_000131"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGC5427264.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLHQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(126965..127981)
                     /gene="ansA"
                     /locus_tag="ACIYSB_000132"
     CDS             complement(126965..127981)
                     /gene="ansA"
                     /locus_tag="ACIYSB_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGC5427265.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(128148..130004)
                     /gene="sppA"
                     /locus_tag="ACIYSB_000133"
     CDS             complement(128148..130004)
                     /gene="sppA"
                     /locus_tag="ACIYSB_000133"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGC5427266.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAEQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQLMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANMR"
     gene            130165..130716
                     /gene="ydjA"
                     /locus_tag="ACIYSB_000134"
     CDS             130165..130716
                     /gene="ydjA"
                     /locus_tag="ACIYSB_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGC5427267.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            130833..131876
                     /gene="selD"
                     /locus_tag="ACIYSB_000135"
     CDS             130833..131876
                     /gene="selD"
                     /locus_tag="ACIYSB_000135"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGC5427268.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            131881..133842
                     /gene="topB"
                     /locus_tag="ACIYSB_000136"
     CDS             131881..133842
                     /gene="topB"
                     /locus_tag="ACIYSB_000136"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGC5427269.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTLLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            133970..135010
                     /gene="ynjI"
                     /locus_tag="ACIYSB_000137"
     CDS             133970..135010
                     /gene="ynjI"
                     /locus_tag="ACIYSB_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGC5427270.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSQMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(135127..136470)
                     /gene="gdhA"
                     /locus_tag="ACIYSB_000138"
     CDS             complement(135127..136470)
                     /gene="gdhA"
                     /locus_tag="ACIYSB_000138"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGC5427271.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSLPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            136706..136978
                     /gene="ynjH"
                     /locus_tag="ACIYSB_000139"
     CDS             136706..136978
                     /gene="ynjH"
                     /locus_tag="ACIYSB_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGC5427272.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(136944..137351)
                     /gene="nudG"
                     /locus_tag="ACIYSB_000140"
     CDS             complement(136944..137351)
                     /gene="nudG"
                     /locus_tag="ACIYSB_000140"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGC5427273.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            137438..138058
                     /gene="ynjF"
                     /locus_tag="ACIYSB_000141"
     CDS             137438..138058
                     /gene="ynjF"
                     /locus_tag="ACIYSB_000141"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGC5427274.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(138067..139374)
                     /gene="ynjE"
                     /locus_tag="ACIYSB_000142"
     CDS             complement(138067..139374)
                     /gene="ynjE"
                     /locus_tag="ACIYSB_000142"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGC5427275.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGLTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(139441..140094)
                     /gene="ynjD"
                     /locus_tag="ACIYSB_000143"
     CDS             complement(139441..140094)
                     /gene="ynjD"
                     /locus_tag="ACIYSB_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGC5427276.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAEQFSCTGELWLNEQRIDILPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLEGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(140094..141629)
                     /gene="ynjC"
                     /locus_tag="ACIYSB_000144"
     CDS             complement(140094..141629)
                     /gene="ynjC"
                     /locus_tag="ACIYSB_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGC5427277.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKWL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDIDQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGNTLAIFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLKLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFAVGIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(141602..142768)
                     /gene="ynjB"
                     /locus_tag="ACIYSB_000145"
     CDS             complement(141602..142768)
                     /gene="ynjB"
                     /locus_tag="ACIYSB_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGC5427278.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDVLHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(142988..143784)
                     /locus_tag="ACIYSB_000146"
                     /pseudo
     CDS             complement(142988..143784)
                     /locus_tag="ACIYSB_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(143869..>143945)
                     /locus_tag="ACIYSB_000147"
     CDS             complement(143869..>143945)
                     /locus_tag="ACIYSB_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427279.1"
                     /translation="LPCIEQAREDIRTLHQLQLLHQNR"
BASE COUNT        36454 a        34992 c        37242 g        35257 t
ORIGIN      
        1 gtgatcatat aatttcaaat gtaattataa gaaaatagtg tactgattct ccgcattaaa
       61 caagtggtta atatatgtaa aacatttcat taacattaag ttgattgttg cctttctttg
      121 tatttaatta gtttgttgtg cggtaagtgt ctgtttaaaa atagcttatc tggtattgca
      181 tgaaagtgat tatttatagc agatgattat ttacggtgag ttattttgac tgtgcgcaac
      241 atcccatttc gattattcct gtttcatttt tgcttgctag cgtagcgaaa aactttttaa
      301 cagattgaaa tacacccaaa acaaaagtat gacttataca tttatgttaa gtaattgagt
      361 gttttgtgtg atctgcatca cacattattg aaaatcgcag cccccctccg ttgtatgtgc
      421 gtgtagtgac gagtacagtt gcgtcgattt aggaaaaatc ttagataagt gtaaagaccc
      481 atttctattt gtaaggacat attaaaccaa aaaggtggtt ctgcttattg cagcttatcg
      541 caactattct aatgctaatt attttttacc ggagcttccc ggcgacatca cggggtgcgg
      601 tgaaaccgca taaaaataaa gttggttatt ttgggtggga ataatgcata cctccgagtt
      661 gctgaaacac atttatgaca tcaacttgtc atatttacta cttgcacagc gtttgattgt
      721 tcaggacaaa gcgtccgcta tgtttcgtct cggcataaat gaagaaatgg cgacaacgtt
      781 agcggcactg actcttccgc aaatggttaa gctggcagaa accaatcaac tggtttgtca
      841 cttccgtttt gacagccacc agacgattac tcagttgacg caagattccc gcgttgacgc
      901 tctccagcaa attcataccg gcatcatgct ctcaacacgc ttgctgaatg atgttaatca
      961 gcctgaagaa gcgctgcgca agaaaagggc ctgatcatga gtgaaaaaag cattgttcag
     1021 gaagcgcggg atattcagct ggcaatggaa ttgatcaccc tgggcgctcg tttgcagatg
     1081 ctggaaagcg aaacacagtt aagtcgcgga cgcctgataa aactttataa agaactgcgc
     1141 ggaagcccac cgccgaaagg catgctgcca ttctcaaccg actggtttat gacctgggaa
     1201 caaaacgttc atgcttcgat gttctgtaat gcatggcagt ttttactgaa aaccggtttg
     1261 tgtaatggcg tcgatgcggt gatcaaagcc taccgtttat accttgaaca gtgcccacaa
     1321 gcagaagaag gaccactgct ggcattaacc cgtgcctgga cattggtgcg gtttgttgaa
     1381 agtggattac tgcaactttc cagctgcaac tgctgcggcg gcaattttat tacccacgct
     1441 caccagcctg ttggcagctt tgcctgcagc ttatgtcaac cgccatcccg ggcagtaaaa
     1501 agacgtaaac tttcccagaa tcctgccgat attatcccac aactgctgga tgaacagaga
     1561 gtacaggctg tttaactgat acggtgaggc gcaacattcc agcagcggta acgacgtacc
     1621 gctgcttttt tttgccccaa tcgcgcgtta acgcctgacg actgaacatc ctgtcatggt
     1681 caacagtgga aggatgatgt cgtgcttatc ttattaggtt acctggttgt tctcggtaca
     1741 gttttcggcg gttatttgat gaccggtgga agccttggag cactctatca acccgctgaa
     1801 ctggtgatta ttgccggtgc agggattggg tcgtttatcg tcggcaataa tggcaaagcg
     1861 attaaaggca cgctgaaggc gctgccgttg ctgtttcgtc gctccaaata caccaaagca
     1921 atgtatatgg atctgctggc tctgctttat cggttgatgg cgaaatcgcg gcagatgggg
     1981 atgttttcgc tggaacgtga tattgaaaat ccccgtgaga gcgagatctt cgccagctac
     2041 ccacgcatcc tcgcggatag cgtcatgctt gattttatcg tcgattatct gcgcctgatt
     2101 atcagcggtc acatgaacac cttcgaaatc gaagctctga tggatgaaga gattgagacg
     2161 cacgaaagcg aggcagaagt cccggcgaac agtctggcgc tggtcgggga ctcacttccg
     2221 gcgtttggta ttgttgcggc tgtaatgggg gtcgttcacg cgttaggttc agccgatcgt
     2281 cctgccgccg agctgggtgc gcttatcgca catgcgatgg tggggacttt cctcggcatt
     2341 ttattggctt acggatttat ttccccatta gcgactgttt tacgtcagaa aagcgccgaa
     2401 accagcaaaa tgatgcagtg cgtcaaagtc actctgcttt ctaatctgaa cggttacgca
     2461 ccgcctatcg ccgttgagtt tggtcgcaaa acgctctatt ccagcgaacg tccgtcgttt
     2521 attgaactgg aagagcatgt gcgtgcggtg aaaaatccgc aacaacagac gacaaccgag
     2581 gaagcatgaa gaatcaagcg catccgatta ttgtcgtcaa acgacgcaaa gccaaaagcc
     2641 acggggcagc acatggatcg tggaagattg cttatgccga ctttatgact gcgatgatgg
     2701 ccttttttct ggtgatgtgg ctgatctcca tctccagccc aaaagagctg attcagattg
     2761 cggagtactt ccggactcca ctggcgactg cggttacggg cggcgatcgc atttctaata
     2821 gtgaaagccc aattcccggc ggtggtgatg attacaccca aagccagggg gaagtgaata
     2881 agcagccgaa catcgaagag ctgaaaaaac gcatggagca aagtcgattg cggaaattgc
     2941 ggggtgatct cgaccagttg atagagtccg atccgaaact gcgggcgtta cgtccccatc
     3001 tcaaaatcga tctggtccag gaaggtctac gtattcagat catcgatagc cagaatcgcc
     3061 cgatgtttag aaccggcagt gccgatgtcg aaccctatat gcgcgacatt ctgcgcgcca
     3121 ttgcgcctgt actgaacggt attcccaacc gtattagcct ttcaggtcat accgatgatt
     3181 tcccctacgc cagcggtgag aaaggatata gcaactggga gctttctgcc gatcgggcca
     3241 atgcatcccg ccgtgaactg atggtcggag ggttggatag cggcaaagtg ttacgtgtcg
     3301 tcggcatggc ggcaacgatg cgcttaagcg atcgcggacc tgatgatgcc gtcaaccgtc
     3361 gcatcagcct gctggtactg aacaaacaag ccgaacaggc cattttgcat gaaaacgccg
     3421 aaagccagaa tgagccagta agcgccctgg aaaaacctga ggttgcacca caggtcagtg
     3481 ttcccacaat gccatcagcc gaaccgaggt gacagcgtga gcatggatat aagcgatttt
     3541 tatcagacat tttttgatga agcggacgaa ctgttggctg acatggagca gcatttgctg
     3601 gttttgcagc cggaagcgcc agatgccgaa caattgaatg ccatctttcg ggctgcccac
     3661 tcgatcaaag gaggggcagg aacttttggc ttcagcgttt tgcaggaaac cacgcatctg
     3721 atggaaaacc tgctcgatga agccagacga ggtgagatgc aactcaacac cgacattatc
     3781 aatctgtttt tggaaacgaa ggacatcatg caagaacagc tcgacgctta taaacagtcg
     3841 caagagccgg atgccgccag cttcgattat atctgccagg ccttgcgtca actggcatta
     3901 gaagcgaaag gcgaaacgcc atccgcagtg acccgattaa gtgtggttgc caaaagtgaa
     3961 ccgcaagatg agcagagtcg cagtcagtcg ccgcgacgaa ttatcctttc gcgcctgaag
     4021 gccggggaag tcgacctgct ggaagaagaa ctgggacatc tgacaacgtt aactgacgtg
     4081 gtgaaagggg cggattcgct ctcggcaata ttaccgggcg acatcgccga agatgacatc
     4141 acagcggtac tctgttttgt gattgaagcc gatcagatta cctttgaaac agtagaagtc
     4201 tcgccaaaaa tatccacccc accagtgctt aaactggcag ccgaacaagc gccaaccggc
     4261 cgcgtggagc gggaaaaaac gacgcgcagc aatgaatcca ccagcatccg tgtagcggta
     4321 gaaaaggttg atcaattaat taacctcgtc ggcgagctgg ttatcaccca gtccatgctt
     4381 gcccagcgtt ccagcgaact ggacccggtt aatcatggtg atttgataac cagcatgggg
     4441 cagttacaac gtaacgcccg tgatttgcag gaatcagtga tgtcgattcg catgatgccg
     4501 atggaatatg tttttagtcg ctatccccgg ctggtgcgtg atctggcggg aaaactcggc
     4561 aagcaggtag aactgacgct ggtgggcagt tctactgaac tcgacaaaag cctgatagaa
     4621 cgcattatcg acccgctgac ccacctggta cgcaatagcc tcgatcacgg tattgaactg
     4681 ccagaaaaac ggctcgccgc aggtaaaaac agcgtcggaa atttaattct gtctgccgaa
     4741 catcagggcg gcaacatttg cattgaagtg accgacgatg gggcggggct aaaccgtgag
     4801 cgaattctgg caaaagcggc ctcgcaaggt ttgactgtca gcgaaaacat gagcgacgac
     4861 gaagtcgcga tgctgatatt tgcacctggc ttctccacgg cagagcaggt caccgacgtc
     4921 tccgggcgcg gcgtcggcat ggacgtcgtt aaacgtaata tccaggagat gggcggtcat
     4981 gtcgaaatcc agtcgaagca gggtactggc actacgatcc gcattttact gccgctgacg
     5041 ctggccatcc tcgacggcat gtccgtacgc gttgcggatg aagttttcat tctgccgctg
     5101 aatgctgtta tggaatcact gcaaccccgt gaagccgatc tccatccact ggccggcggc
     5161 gagcgggtgc tggaagtgcg gggtgaatat ctgcccatcg tcgaactgtg gaaagtgttc
     5221 aacgtcgcgg gcgcgaaaac cgaagccacc cagggaattg tggtgatctt acaaagtggc
     5281 ggtcgccgct acgccttgct ggtggatcaa ttaattggtc aacaccaggt tgtggttaaa
     5341 aaccttgaaa gtaactatcg caaagtcccc ggcatttctg ctgcgaccat tcttggcgac
     5401 ggcagcgtgg cactgattgt tgatgtctcc gccttgcagg cgataaaccg cgaacaacgt
     5461 atggcgaaca ccgccgcctg aatgagtaaa aaggtaacaa tatgaccggt atgacgaatg
     5521 taacaaagct ggccagcgag ccgtcaggcc aggaatttct ggtatttacc cttggtgatg
     5581 aagagtacgg tattgatatc ctgaaagtgc aggagatccg tggctacgat caggtaacac
     5641 ggattgcgaa cacgccagcg tttatcaaag gcgtcacgaa tctgcgcggc gttattgtgc
     5701 cgattgttga cttacgaatt aagttcagcc aggtggatgt ggactataac gacaacacgg
     5761 tagttatcgt cctgaatctc ggacagcggg tggtcggcat cgtggttgac ggcgtctcag
     5821 acgtgctttc attgacggcg gagcaaattc gtccggcacc ggaatttgcc gtgacgcttt
     5881 caacagaata tctcactgga ctgggcgcac tgggcgaccg gatgttgatt ctggtgaaca
     5941 tcgaaaaact gctgaacagc gaagagatgg cgctgttaga tagcgcggcg tcagaagtgg
     6001 cgtaattctc ccgatttcct caattgaaat gaacccgatg atctgcgcat cgggtttttt
     6061 atttcaattt cgcggcgggt ggcatcagca ataaagtttc ccccctcctt gccgataacg
     6121 agatcaactt gttttcagga aggtgcctta tgattaaccg tatccgcgta gtcacgctgt
     6181 tggtaatggt gctgggggta ttcgcactgt tacagcttat ttccggcagt ctgttttttt
     6241 cttcccttca ccatagccag aagagctttg tggtttccaa tcaattacgg gaacagcagg
     6301 gcgagctgac gtcaacctgg gatttaatgc tgcaaacgcg cattaacctg agtcgttcag
     6361 cggtacggat gatgatggat tcctccaatc aacaaagtaa cgccaaagtt gaattgctcg
     6421 atagcgccag gaaaacattg gcgcaggcag cgacgcatta taaaaaattc aaaagcatgg
     6481 caccgttacc tgaaatggtc gctaccagtc gtaatattga tgaaaaatat aaaaactatt
     6541 acacagcgtt aactgaactg attgattatc tagattatgg caatactgga gcttatttcg
     6601 ctcagccaac ccagggaatg caaaatgcaa tgggcgaagc gtttgctcag tacgccctca
     6661 gcagtgaaaa actgtatcgc gatatcgtca ctgacaacgc agatgattac cgatttgccc
     6721 agtggcaact ggcggttatc gcgctggtgg tggtattgat tctgctggtg gcgtggtacg
     6781 gcattcgccg tatgttgctt actccgctgg caaaaattat tgctcacatt cgcgaaatcg
     6841 ccggtggtaa cctggcgaat accctgacca ttgacgggcg cagtgaaatg ggcgacctgg
     6901 cgcagagcgt ttcacatatg caacgctctt tgactgacac cgtcactcat gtccgcgaag
     6961 gttcagatgc catctatgcc ggtacccgtg aaattgcggc gggcaacacc gatctttcct
     7021 cccgtactga acagcaggca tccgcgctgg aagaaactgc cgccagcatg gagcagctca
     7081 ccgcgacagt gaagcaaaac gccgataacg cccgccaggc ctcgcaactg gcgcaaagtg
     7141 cctccgacac cgcccagcac ggcggcaaag tggtggatgg cgtagtgaaa acgatgcatg
     7201 agatcgccga tagttcgaag aaaattgccg acattatcag cgttatcgac ggtattgcct
     7261 tccagactaa tatcctcgcg ctgaatgccg cggttgaagc cgcgcgtgcg ggtgaacagg
     7321 gccgtggttt tgccgtggtg gcgggtgaag tgcgtaatct tgccagtcgc agcgcccagg
     7381 cggcaaaaga gatcaaagcc ctcattgaag actccgtctc acgcgttgat accggttcgg
     7441 tgctggtcga aagcgccggg gaaacaatga acaatatcgt caatgctgtc actcgcgtga
     7501 ctgacattat gggcgagatt gcatcggcat cggatgaaca gagccgtggc atcgatcaag
     7561 tcgcattggc ggtttcggaa atggatcgcg tcacgcaaca gaacgcatcg ctggtgcagg
     7621 aatcagctgc cgccgccgct gcgctggaag aacaggcgag tcgtttaacg caagcagttt
     7681 ccgcgttccg tctggcagcc agcccactca ccaataaacc gcaaacacca tcccgtcctg
     7741 ccagtgagca accaccggct cagccacgac tgcgaattgc tgaacaagat ccaaactggg
     7801 aaacattttg acgagtattt actaacgcgg tcattgccgc ctgatgggga gcgttgatgt
     7861 ttaatcgtat tcgaatttcg accacgctgt ttttaatttt gattctctgc gggatcttgc
     7921 agattggcag taacggcatg tctttttggg catttcgcga cgatttgcaa cgactgaatc
     7981 aggtcgagca gagcaatcag caacgtgcgg cattagcgca aactcgggcg gtaatgttac
     8041 aggccagtac cgcgctgaac aaagcgggca ctctgacggc acttagctat ccggcggatg
     8101 acattaaaac gttgatgacg acggcgcgcg ccagtctgac gcaatccacc acgctgttta
     8161 aaagttttat ggcgatgact gcgggcaacg agcacgtcag gggattgcaa aaagagacgg
     8221 agaaaagttt tgcccgctgg cacaacgatc tcgaacatca ggcgacctgg cttgaaagta
     8281 atcaactttc ggatttcctc actgcgccgg tgcagggatc acagaatgcg tttgacgtta
     8341 actttgaggc ctggcagctg gagatcaacc atgtgctgga agccgccagt gcgcaaagcc
     8401 agcgtaacta tcagatttcg gcactggtgt ttatcagcat gattattgtt gcggcgatct
     8461 acatcagcag tgcgctgtgg tggacgcgca agatgattgt tcaaccactg gccattatcg
     8521 gtagccattt tgacagcatt gctgcgggta atctggcgcg tccgattgcg gtatatggtc
     8581 gtaatgagat caccgccatt tttgccagtc tgaagaccat gcagcaggct ttgcgtggga
     8641 cggtaagtga tgtgcgtaag ggaagccagg agatgcacat tggtatcgcg gagattgtcg
     8701 caggcaataa cgatctctca agtcgtaccg aacagcaggc ggcatcgctg gcacaaacgg
     8761 ccgccagtat ggagcaatta accgccacgg tagggcaaaa cgccgataac gcacgacagg
     8821 cgtcggaact ggcaaaaaat gccgcgacaa cggcgcaggc gggcggtgtt caggtcagta
     8881 ccatgactca caccatgcag gagatcgcca ccagctcgca aaaaattggc gacattatca
     8941 gcgttatcga cggaattgct ttccagacca atattctggc cctgaatgcg gcagtggaag
     9001 cggctcgcgc cggagagcag gggcgtggtt ttgcggtagt ggcaggtgaa gtgcgcaatc
     9061 ttgccagccg tagcgcgcag gcagcaaaag agatcaaagg gctgatcgaa gagtcagtca
     9121 atcgtgtcca gcagggttcg aaactggtga ataacgccgc cgcgaccatg atcgatattg
     9181 tcagttcggt gacccgcgtg aacgacatta tgggagaaat tgcctctgcg tcggaagaac
     9241 aacagcgggg gattgagcag gttgcacagg ctgtcagcca gatggatcag gtgactcagc
     9301 agaacgcctc gctggtagaa gaagcggcgg tggcaacgga acaactggcg aatcaggccg
     9361 accatctttc gtcgcgtgtg gcggtattta cccttgaaga acatgaagta gcacgacatg
     9421 agtcggtgca gttacaaatt gcgccagtgg tatcctgaag tgattgagaa ggcgctatga
     9481 cttcatctct gccctgtggg caaacgtctt tattgttaca gatgaccgag cgcctggcgc
     9541 tttccgacgc gcattttcgg cggataagtc aattgatcta tcaacgagcc gggatcgttc
     9601 tggctgacca taaacgcgac atggtttaca accgactggt tcgtcgtttg cgttcgctgg
     9661 gactgacgga tttcggtcat tatctgaact tgctggaatc taatcagcac agcggtgagt
     9721 ggcaggcgtt tatcaattcg ctgaccacga atctgacggc atttttccgt gaggcacatc
     9781 atttccctct gctcgcggat cacgcacgtc gccgttctgg cgagtatcgc gtatggagcg
     9841 cggcggcttc gaccggcgaa gagccgtaca gcattgcgat gacgctggct gacacattgg
     9901 gcaccgcgcc cggacgctgg aaagtgtttg ccagtgatat cgacaccgaa gtgctggaaa
     9961 aagccagaag cggtatctat cgccatgaag agttgaaaaa cctgacgccg cagcaactgc
    10021 aacggtattt catgcgaggg acggggccgc atgaagggct ggtacgcgtg cgtcaggagc
    10081 tggcgaacta tgttgatttt gccccgctga atctactggc gaaacagtac accgtgccgg
    10141 ggccgtttga tgcgatcttc tgtcgtaacg tcatgatcta cttcgatcaa actacccagc
    10201 aggagatttt gcgccgcttt gttccgctcc ttaaacccga cggattgctg tttgcgggtc
    10261 actctgaaaa ctttagccac cttgagcgcc gcttcacgct gcgtggtcag acggtgtatg
    10321 cgctaagtaa ggattaacga tgagcaaaat cagggtgtta tctgtcgatg attcggcact
    10381 gatgcgccag atcatgacag aaatcatcaa cagccatagc gacatggaaa tggtggcgac
    10441 cgcgcctgat ccgctggtcg cgcgtgactt gattaagaaa ttcaatcccg atgtgctgac
    10501 gctggatgtt gaaatgccgc ggatggacgg actggatttc ctcgaaaaat taatgcgttt
    10561 gcgtccaatg cccgttgtga tggtttcttc cctgaccggc aaagggtcag aagtcacgct
    10621 gcgcgcgctg gagctggggg cgatagattt tgtcaccaaa ccgcaactgg gtattcgcga
    10681 aggtatgctg gcgtataacg aaatgattgc tgaaaaggtg cgtacggcag caaaggcgag
    10741 ccttgcagca cataagccat tgtcggcacc gacaacgctg aaggcggggc cgttgttgag
    10801 ttctgaaaaa ctgattgcga ttggtgcttc aacgggtgga actgaggcaa ttcgtcacgt
    10861 actgcaaccg ttgccgcttt ccagcccggc actgttaatt acccagcata tgccgcccgg
    10921 tttcacccgc tcttttgccg acagacttaa taagctttgc cagatcgggg ttaaagaagc
    10981 cgaagacgga gaacgtgtct tgccggggca tgcctatatt gcgccgggcg atcggcatat
    11041 ggagctgtcg cgtagtggcg caaattacca aatcaaaatt cacgatggcc cggcggttaa
    11101 ccgtcatcgg ccttcggtag atgtgttgtt ccattctgtc gccaaacagg cggggcgtaa
    11161 tgcggttggg gtgatcctga ccggtatggg caacgacggc gcggcgggaa tgttggcgat
    11221 gcgtcaggcg ggggcatgga cccttgcgca aaacgaagca agttgcgtgg tgttcggcat
    11281 gccgcgcgag gccatcaata tgggtggtgt ctgcgaagtg gtcgatctta gccaggtaag
    11341 ccagcaaatg ttggcaaaaa ttagtgccgg acaggcgata cgtatttaaa tcaggagtgt
    11401 gaaatggcgg ataaagaact taaatttttg gttgtggatg acttttccac catgcgacgc
    11461 atagtgcgta acctgctgaa agagctggga ttcaataatg ttgaggaagc ggaagatggc
    11521 gtcgacgctc tcaataagtt gcaggcaggc ggttatggat ttgttatctt cgactggaac
    11581 atgcccaata tggatggcct ggaattgctg aaaacaattc gtgcggatgg cgcgatgtcg
    11641 gcattgccag tgttaatggt gactgcagaa gcgaagaaag agaacatcat tgctgcggcg
    11701 caagcggggg ccagtggcta tgtggtgaag ccatttaccg ccgcgacgct ggaggaaaaa
    11761 ctcaacaaaa tctttgagaa actgggcatg tgaggatgcg actatgatgc aaccatcaat
    11821 caaacctgct gacgagcatt cagctggcga tatcattgcg cgcatcggca gcctgacgcg
    11881 tatgctgcgc gacagtttgc gggaactggg gctggatcag gccattgccg aagcggcgga
    11941 agccatcccc gatgcgcgcg atcgtttgta ctatgttgtg cagatgaccg cccaggctgc
    12001 ggagcgggcg ctgaacagtg ttgaggcgtc acaaccgcat caggatcaaa tggagaaatc
    12061 agcaaaagcg ttaacccaac gttgggatga ctggtttgcc gatccgattg accttgccga
    12121 cgcccgtgaa ctggtaacag atacacgaca atttctggca gatgtacccg cgcataccag
    12181 ctttactaac gcgcaactgc tggaaatcat gatggcgcag gattttcagg atctcaccgg
    12241 gcaggtcatt aagcggatga tggatgtcat tcaggagatc gaacgccagt tgctgatggt
    12301 gctgttggaa aacatcccgg aacaggagtc gcgtccaaaa cgtgaaaacc agagtttgct
    12361 taatggacct caggtcgata ccagcaaagc cggtgtggta gccagtcagg atcaggtgga
    12421 cgatttgttg gatagtcttg gattttgatt tgtattgcct gatgtggcgt gaccacgtca
    12481 tatcaggcgg tctgataagg cgatgacgcc gcatccgaca aacgcacact atgcttgatg
    12541 tgtggcagca aaagccctaa atcccgcctg ttttgcccct tactcaaacc attgaacgct
    12601 ttgcgctctg gcatcattca cgcttaatac tctttccagg attggcgacg tgtctgacga
    12661 gagcgacgac aaaacagaag cccccacacc tcaccgacta gaaaaagcgc gggaagaggg
    12721 gcaaatcccg cgttcccgtg aactgacctc actgctgatt ttgctggtgg gcgttagtgt
    12781 tatctggttt ggcggtgtgt cgctggcccg tcgattgtcg ggcatgctct ccgctgggct
    12841 gcattttgat cacagtatta tcaatgaccc gaatctgatc ctcgggcaga ttattctgct
    12901 gatcagagaa gccatgctgg cgctgctgcc gctgattagc ggcgtggtgc tggtggcgct
    12961 catttctccg gtcatgctgg gagggctggt atttagcggc aaatccttgc agccgaagtt
    13021 ttccaaactc aacccgctac cgggcattaa acggatgttc tcggctcaga ctggcgcgga
    13081 gttgcttaaa gcaattttga aaaccattct ggttggcagc gtgacggggt tttttctctg
    13141 gcatcactgg ccgcagatga tgcgcttgat ggccgagtct ccgattaccg ccatgggtaa
    13201 tgcgatggat ttggtagggc tatgcgcact gctggtggtg cttggtgtca ttccaatggt
    13261 gggatttgac gtctttttcc aaatattcag ccacctgaaa aagctgcgta tgtcacggca
    13321 ggatattcgt gatgagttca aacaaagcga aggtgaccct catgttaaag ggcggatccg
    13381 tcagatgcag cgagctgctg cacggcgtcg gatgatggcc gatgtgccga aagcggatgt
    13441 cattgtcaat aacccgaccc actattcggt agcgttgcag tatgacgaaa acaaaatgag
    13501 cgcaccgaaa gtggtcgcta aaggtgcagg gctggtcgcg ctgcgcattc gtgaaattgg
    13561 cgctgaaaat aacgtcccga cgcttgaagc gccgccgctg gcgcgagcgc tgtatcgaca
    13621 tgcggagatt ggtcaacaaa tcccgggtca actgtacgcc gcggtggcgg aagtgctggc
    13681 ctgggtctgg caactgaaac gctggcgtct ggctggtgga cagcgccctg tacaacctac
    13741 tcatcttccg gtgccggaag ccctggattt tattaacgag aaaccgaccc atgagtaatc
    13801 tggccgcgat gctgcgcctg cccgcaaacc tgaaatcgac acaatggcag atccttgccg
    13861 gaccgatttt gatcctgttg atcttgtcga tgatggtgct gccactgccc gcattcatac
    13921 tcgacctgtt gtttaccttc aatattgcct tgtcgatcat ggtgttgctg gtggcgatgt
    13981 ttacccagcg cacgcttgag tttgctgcgt ttccgaccat tctgttgttt accacgctgt
    14041 tgcgtctggc acttaacgtg gcttcaaccc gtatcatttt aatggaaggg cataccggcg
    14101 cggcggcggc agggaaggtg gtcgaagcgt tcggtcactt cctcgttggt ggcaatttcg
    14161 ctatcggtat cgtggtgttt gtcattctcg tgatcatcaa ctttatggtc attaccaaag
    14221 gtgccgggcg tatcgcagaa gtgggtgcgc gctttgttct cgatggtatg ccgggtaagc
    14281 agatggcgat tgacgccgac cttaacgccg gattgattgg tgaagatgag gcgaaaaaac
    14341 gccgctccga agtgactcag gaagccgatt tttacggctc aatggacggg gcaagtaagt
    14401 ttgttcgcgg cgatgccatc gccgggatcc tcatcatggt cattaacatt gtcggcgggt
    14461 tgctggtcgg cgtgctgcaa catggcatga gcatgggaca cgcggcggaa agttatacgc
    14521 tattgaccat tggcgacggt ctggtggcac aaattccggc gctggtgatt tctaccgccg
    14581 cgggggtcat cgttacgcgt gtcagcaccg atcaggatgt tggcgagcag atggtgaatc
    14641 agcttttcag taacccaagc gttatgttgt taagcgccgc cgtgctcggt ttactcggcc
    14701 tggtgcctgg aatgccgaac ctggtatttt tgctgttcac tgccggattg ctcgggctgg
    14761 cctggtggat acgcggacgc gaacaaaaag cgcctgccga acccaaaccg gtaaaaatgg
    14821 cagagaataa taccgttgtc gaagcgacgt ggaacgatgt acaactggaa gattctctgg
    14881 gaatggaagt gggttatcga ctgatcccga tggtcgattt ccagcaggat ggtgagttgt
    14941 tgggccgtat acgcagtatc cgcaagaaat ttgcccagga gatgggattt ctgccgccag
    15001 tggtgcacat tcgcgacaat atggatctgc aacctgcccg ctatcgcatt ttgatgaaag
    15061 gcgtggagat tggcagtggt gatgcttatc cggggcgctg gctggcgatt aaccctggaa
    15121 ccgctgccgg gacgttacct ggtgaggcga ccgtcgatcc ggcatttggc ctgaatgcta
    15181 tctggattga aagtgcgcta aaagaacagg cgcagattca ggggtacaca gtggttgagg
    15241 ccagcacggt ggtagcaacg catcttaacc acctcattag ccagcatgcc gcagagctgt
    15301 ttggtcgtca ggaggcgcaa cagctgttgg atcgcgtcgc ccaggagatg ccaaagctga
    15361 cggaagatct cgttcctggc gtcgtcacgc tcaccacact gcataaagtg ctgcaaaatc
    15421 tcctcgatga aaaagtaccg attcgcgata tgcgcaccat tctcgaaacg ctggcggaac
    15481 atgcgcccat ccaaagcgat ccacatgaat taaccgccgt cgtgcgcgtg gcgttgggac
    15541 gggcgattac ccagcagtgg tttcctggca aagatgaagt ccatgttatt ggcctcgata
    15601 caccgctgga acgtttgtta ctacaggcgc tgcagggcgg gggaggactg gagccagggc
    15661 tggcggatcg tttactggcg caaactcagg aagcgctatc ccgtcaggag atgctgggtg
    15721 cgccgccagt attgttggtg aaccacgcgc tgcgaccatt attgtctcgc ttcctgcgcc
    15781 gcagcttgcc gcagttagtg gtcctgtcga atctggaact gtctgataac cgacatatcc
    15841 gcatgacggc gacaattggc ggcaaataat gagaacctta ttagcaatat tattgtttcc
    15901 gctgctggtg caagccgccg gggaggggat gtggcaggca agtagtgtag gaattacgct
    15961 aaatcatcgc ggtgagtcga tgtcgtctgc gcctctttct acgcgacaac ctgcttcagg
    16021 attgatgacg ctggtagcgt ggcgttatca gcttatcggc ccgacacctt caggactgcg
    16081 ggttcgcttg tgttcgcaat ctcgttgtgt cgaattagag gggcagagcg gaaccaccgt
    16141 ggccttttcc ggtatagcgg cagcagaacc gttgcgattt atctgggaag tgccaggcgg
    16201 tgggcggtta attccaccgc taaaggtaca acgtaatgaa gtgattgtga attatcgctg
    16261 aaggacaaaa aaattacgcg ctcgcgagta tgtttaataa ctcagtgaaa aaggctcgat
    16321 gtcttgttgc aattatttat caggaatagc ttgcaacaaa acatgccaaa aagatgtgaa
    16381 ttatcctctt gtcaaagggc ttggataaaa atcttcagag ttacggtata gctgcaactg
    16441 gcgagttcgc gtattggtct gtatgatcat acatgagagc cattctgatt cagaataagg
    16501 ggcacttaaa tcctgaacgt cctcatttga aaggattaat tcacattctt tgtccctaaa
    16561 atccagccag gctcgctgtg attttttaag caagttaagt tgttcggtat caggtttttt
    16621 tacacctgtg cgtggcactt gttctatata acgataagct tcaccatagg ctttatttaa
    16681 ttgcgcatcg acgagattgt attcttgtcg caggcatgat ttgatcacgt taacgtcagt
    16741 atcatcatct gaattttttt gccaacaatc tttgatggct gaaatgtcat ggtcgtttac
    16801 tataaccgta tcttctgcgt aagcctggca agagataatg cctaatgtta atacaagaaa
    16861 tttaaacact gcggtattcc ttaaattcaa ccgttatcgt ttccctgggg ccggatatta
    16921 cgcttctggc ctcttatcca acagtgagaa aacaatggca tgtccatttt cagaatacat
    16981 ttagatggca ataaaaaagc ctgatcggct aaccctgatc aggcttcact ctcacgaaaa
    17041 atcgattaca tacgctctac agtctcaata cccagcgtat ccagacccag cttcagcgtc
    17101 ttcgccgtca gttgtgccag ttttagacgg ctgttacgca cttcttcgtt ttctgcgctg
    17161 aggatcgggc agtgctcgta gaagccagag aacagaccgg ccagatcgta caggtaagca
    17221 cacattacat gcggcgtgcc ttcacgggca accacggtga gggtttcttc aaactgcagc
    17281 aggcgagctg ccagttgcgc ttcacgatct tcacggatga taaccggagc tgcagccagt
    17341 tgctcttcgt caatttctgc tttacggaac acggacaata cacgcgtgta tgcatactgc
    17401 atgtatggcg cggtattacc ctcaaacgcc agcatgttgt cccagtcgaa gatgtagtcc
    17461 gtggtgcggt ttttggagag atccgcatat ttcaccgcac caataccaac cgcgttagcc
    17521 agtttttcca gctcgtcggc tggcatatcc gggttctttt ctgccaccag acggcgtgca
    17581 cgttccaggg cttcatccag cagatcggcc agtttcactg taccacccgc gcgggttttg
    17641 aacggtttgc cgtctttacc cagcatcatg ccgaacatgt ggtgttccag cggtacggat
    17701 tccggtacat agcctgcttt acggacgatc gcccatgcct gcatcaggtg ttgatgctga
    17761 cgggagtcga tgtaatacag cacgcgatcg gcatgcagtg tttcataacg atatttcgca
    17821 caggcgatat cagtggtggt gtagagatag ccgccatctt tcttctgaat gatcacgccc
    17881 atcggttcgc cttccttgtt tttaaactca tcaaggaata cgacggtcgc cccttcgctt
    17941 tctactgcca gacctttggc tttgagatcc gccacaattc ctggcagcat cgggttgtag
    18001 aggctttcgc ccatcacgtc atcacgggtc agcgtcacgt tgagacgatc gtaggtgatc
    18061 tggttctgcg tcatggtgat gtcgaccagt ttgcgccaca tctcgcggaa atattcgtca
    18121 ccgctttgca gttttaccac gtagttacgt gcgcgctcgg cgaactcttc atcttcatcg
    18181 taatgctttt tcgcatcgcg gtagaaacct tcaaggtcag ccagctccat ttcaccggcg
    18241 ttttcctgct gctgcttttc cagccatgca atcagcatac cgaactgagt gccccagtcg
    18301 ccgacgtggt ttgcgcgaat cactttgtga ccgaggaact ccagagtacg cactgctgcg
    18361 tcaccaataa tggtagagcg caggtgaccg acatgcatct ctttcgccac gtttggcgca
    18421 gagtagtcaa ccacaatggt ctgtttttct ggcgtagcaa caccgagacg atcggacgcc
    18481 agcgcctgct gaacatgttc agccaggaat gccggatcaa ggaaaatgtt gataaagcct
    18541 ggaccggcga tctcaacttt gctggcgata ccgttaagat ccagatgagt cagcacctgc
    18601 tctgctaatt gtcgcggtgc catacccagt tttttagcaa ctgccatcat gccgttagcc
    18661 tgatagtcgc cgaactgaac ttttgctgac tgacgaacct gcggttcgca atccgcaggc
    18721 gcgcctgccg caatcatggc ctgacggact ttttctgaga gaagagcctg aatattcacc
    18781 ggaatacctt acattgatga agcgagcaaa aattgcccgc accagttaaa gaaattaggg
    18841 cgggagtata ctgcaaatgg ctgttttcgt cagcaacgtg cgcgtggcgt attgccgttt
    18901 tcactattgc atcagaagcg gcgtgaaatc agcggttggt tggcacaggg caacagggta
    18961 aattaacgct tttactgaaa gacgaagagt gattatggcg aactggcaat ctatcgacga
    19021 gctgcaggat attgcatcgg atttaccgcg atttatccac gcattagatg aactttcccg
    19081 ccgtctgggg ttgaatatca ctccgctgac tgccgatcac atttctttgc gttgccatca
    19141 aaacgccact gctgaacgct ggcgtcgcgg gtttgaacag tgtggcgagc ttttgtcaga
    19201 aaatatgatc aatggcagac cgatttgtct gtttaaattg catgaaccgg tacaggttgc
    19261 gcactggcag ttttctattg tggagctacc gtggcccggg gaaaaacgtt acccacacga
    19321 aggttgggaa catattgaaa tcgttctgcc gggtgatcca gaaacgctga atgcccgtgc
    19381 gctggcattg ctttctgatg aaggactcag cctaccgggg atctcagtga aaaccagttc
    19441 gccaaaaggt gagcatgagc gattaccgaa tccgacgctg gcggtcaccg atggcaaaac
    19501 aactattaag tttcatccgt ggtccattga agagatcgtt gccagcgagc agtcggcgta
    19561 attaaggagt aaaaatggca ttactggaaa tttgctgtta cagcatggaa tgtgcactaa
    19621 cggcgcagca aaacggcgca gacagagttg aattatgcgc agccccaaaa gaggggggct
    19681 taacgccgtc gttgggtgta ctgaaatccg tgcgccagcg ggtgacgatc cctgtgcatc
    19741 cgataattcg cccacgcggt ggtgattttt gctacagcga cggtgagttt gccgccattc
    19801 ttgaggatgt gcgcacggtc cgcgaattag gttttcctgg actggtgacg ggcgttctcg
    19861 atgttgacgg gaatgtcgat atgccacgaa tggaaaaaat aatggctgct gccggtccgc
    19921 tggcagtgac ttttcatcgc gccttcgata tgtgcgctaa ccctttatat acactcaata
    19981 atcttgcgga attgggcatt gcccgggtac tgacatcagg gcaaaaatca gacgcactgc
    20041 aaggtttatc aaaaattatg gaacttattg cccatcgtga tgctccaatc attatggccg
    20101 gagcaggagt ccgtgcagaa aacctgcacc acttcctcga tgccggagtg ctggaagtcc
    20161 atagctccgc gggagcgtgg caagcctcac cgatgcgtta tcgtaatcaa ggattgtcca
    20221 tgtcatcaga tgaacacgcg gacgagtatt cgcgttatat cgtagacggg gcggcggttg
    20281 ctgaaatgaa aggaatcatt gaacgccatc aggccaaatg atttttaccg ttgcatcatg
    20341 tcgcccaata tgatgcttgc tcgtaccagg cccctgcaat ttcaacaggg gccttttttt
    20401 atccctgaac agtataaaaa acgaacgata accgtgatct gttgagcggg tgacagtgcg
    20461 catagcgttg tgctaaaaat attgtatata ttcacattaa ttatgggatt taaattacta
    20521 aaactgataa atatatattc taaatagcaa ctgggttatt ccttagcaat taatgattac
    20581 attgtaataa atcatattct ttatcgattg tttcaggcag tgttgtgcct aattatgcaa
    20641 gcggttaatt cgttgtatat ttaattatac aatgatttcg gtgtccagta atttaattag
    20701 aggaatctat gcgagggaaa aaacgcattg ggttattgtt tttgctgata gcggttgtgg
    20761 ttggtggcgg cgggttattg ctggcgcaaa aagccttaca taaaacgtcg gatacagcat
    20821 tttgcctttc ctgccactcg atgagtaaac cttttgagga atatcaggga actgtccact
    20881 tttcgaacca gaaagggata cgtgcggaat gtgccgattg ccatattcca aagtcaggga
    20941 tggattattt atttgctaaa ttaaaggcat ctaaagatat ttatcatgaa tttgttagcg
    21001 gcaaaataga cagtgacgat aagttcgaag ctcatcgcca ggaaatggcc gaaacagtat
    21061 ggaaagaatt aaaagccact gactctgcaa cgtgccgtag ttgccattct tttgatgcca
    21121 tggatattgc ctcgcaaagt gaatctgcgc agaaaatgca taacaaagca caaaaggaca
    21181 gcgaaacctg tatcgattgt cataaaggca ttgcccattt tccgccagaa ataaaaatgg
    21241 atgacaacgc ggcgcatgag ctggaaagtc aggccgctac ttcagttact aatggcgcac
    21301 atatttatcc tttcaaaact tctcacatag gcgagctggc taccgtgaat cctggaaccg
    21361 atctcaccgt cgttgatgcc agtggcaaac agccgatcgt tctgttgcag ggttatcaaa
    21421 tgcagggcag tgaaaacacg ctctacctgg cggcaggtca acggctggcg ctagccacat
    21481 taagtgaaga aggtatcaag gcgctcactg taaacgggga atggcaggct gacgaatacg
    21541 gcaatcaatg gcgtcaggcg tctttacagg gtgcgcttac cgatcccgca ttagcggacc
    21601 gtaaaccgct atggcaatac gctgaaaaac ttgacgatac ctattgcgct ggttgtcatg
    21661 cccctattgc cgccgaccat tacaccgtca atgcgtggcc gtccattgcc aaaggaatgg
    21721 gggcacgaac cagcatgagc gaaaacgaac tggacatttt aacgcggtat ttccagtaca
    21781 acgccaaaga tattaccgag aaacagtgat cctcgttgat cagggaggaa gttatgacat
    21841 taacaagacg tgaatttatt aaacacagcg gtattgccgc tggagcgctg gtggtgacat
    21901 cggcagcacc gctccccgca tgggcagaag agaagggcgg taaaatcctc accgcgggac
    21961 gttggggagc gatgaacgta gaagtgaagg acggcaagat tgtttcttca acaggcgcgc
    22021 tggcgaaaac cataccgaat tccttacagt ctacggcggc ggatcaggta cacaccacgg
    22081 cgcgtattca gcatccgatg gtgagaaaaa gctatctcga taatccactg caaccggcga
    22141 aaggtcgtgg cgaagatacc tatgtacagg tgagctggga acaggcatta aaactgattc
    22201 atgagcaaca tgatcgtatt cgtaaatcta acggaccatc ggccattttt gccggatctt
    22261 acggctggcg ctcttctggc gtgttgcata aagcgcaaac cttactgcaa cgttatatga
    22321 acctggcggg cggttattcc gggcatagcg gcgattattc aaccggggca gcacaggtga
    22381 tcatgccgca tgtggtcggt tctgtagagg tgtatgaaca gcagacctcc tggccgctga
    22441 ttctggaaaa cagccaggtt gtggtgctgt ggggaatgaa cccgctgaat accttaaaaa
    22501 ttgcctggag cagtaccgat gagcaagggc tggaatactt ccatcagctg aaaaaatctg
    22561 gcaaaccagt gattgccatt gatcctatcc gctccgaaac catcgaattc tttgacgata
    22621 atgccacctg gatcgcgccg aatatgggca ccgacgtggc actgatgtta gggattgcgc
    22681 ataccctgat gacacaaggc aaacacgata aagtgttcct tgaaaaatac actaccgggt
    22741 atccgcagtt tgaagagtat ctgacaggta agagcgataa tacgccgaag agcgcggtct
    22801 gggcggcgga aattactggc gtaccggaag cgcagatagt caaactcgct gaactgatgg
    22861 cggcgaatcg cacaatgctg atggcaggct ggggaattca gcgccagcaa tatggtgaac
    22921 agaaacactg gatgctggtg acgctggcgg cgatgttagg gcagattggt acaccgggcg
    22981 gcggttttgg tttctcttat cattactcca acggtggcaa cccgacgcgt gttggcggcg
    23041 tgctgccgga aatgtctgcg gcgattgccg gacatgccag cgaggccgct gatgatggcg
    23101 gaatgacggc gattcctgtt gcccgtatcg tcgatgcgct ggaaaatcct ggcggcaaat
    23161 atcagcataa cggtaaagaa caaacgtatc cgaatatcaa aatgatctgg tgggcgggtg
    23221 gcggtaactt tacccatcac caggatacta atcgtctgat taaagcatgg cagaaaccgg
    23281 agatgatcgt cgtttctgaa tgctactgga ccgcagcagc caaacacgca gatatcgtat
    23341 taccgatcac cacatcgttt gagcgcaatg acttgacgat gaccggtgat tacagcaacc
    23401 agcatattgt gccgatgaag caggctgttg ctccgcaatt tgaagcgcgt aacgattttg
    23461 acgtgtttgc cgatcttgcg gaattactca aacctggcgg aaaagagatc tataccgaag
    23521 gtaaagatga aatggcgtgg ctgaaatttt tctatgatgc cgctcagaaa ggtgcccgtg
    23581 cgcaacgcgt cactatgcca atgtttaatg ccttctggca gcaaaataaa ctgatcgaaa
    23641 tgcgccgcag cgagaagaac gaacagtacg ttcgttatgg tgatttccgc gccgatccgg
    23701 tgaaaaatgc gctgggtacg ccaagcggca aaattgaaat ctattcaaaa acgttggaaa
    23761 aatttggcta taaggattgc ccggcacacc caacctggct ggctcctgat gaatggaaag
    23821 gtaccgccga cgagaagcag ttacagcttc tgaccgcaca tccggcacac cgtttacaca
    23881 gtcagcttaa ctatgcggaa ctgcgtaaaa aatatgcgat cgccgatcgt gaaccaatca
    23941 ctattcacac cgaagatgct gcacgctttg gtattgcgaa tggcgatctg gtgcgtgtct
    24001 ggaacaaacg tggtcagatt ttgacaggcg cggtggtgac tgacgggatc aaaaaaggcg
    24061 tggtatgcgt gcatgaaggt gcatggccag atctggaaaa tggcttgtgt aaaaacggca
    24121 gtgcgaacgt gttaacggcg gatatcccca gctcgcagct ggcaaatgcc tgtgccggta
    24181 actcagcgtt ggtgtatatc gaaaaatata cgggcaatgc accgaagtta acggcgtttg
    24241 atcagccagc tgttcaggcg taagacgtga atagcgcccg gaaacgggcg ctgaggttat
    24301 gcaaacgtaa agctgcctga tgcgctatgc ttatcaggcc tacatagtcc ctgcaatata
    24361 ttgaattagc aaggttttgt aggccggata aggcgttcac gccgcatccg gcagtaatac
    24421 cagaccttta cggcttgcgc gcaatcagca ccgcgcgttt aggtgccgga taaccttcca
    24481 ccgttttacc cggatcatgt gggtcgagga aatcggccag cgattcggta accatccatt
    24541 cggtgcgtcg ctgctcttct gtggtggtaa cgctcacatc cgcaatgcgg atatctacaa
    24601 aaccacactt cttcagccag tttttcagcg ccagcgcgga agggatgaaa tagacattac
    24661 gcatttgcgc gtaacgatcg cctggtacca gcaccgtgtt ctcatcacca tcaataacca
    24721 gcgtttccag caccagttca ccttcattga ccagttgatc tttcaactgc catagatgct
    24781 ccagcggtga acggcgatgg taaagcacgc ccatcgaaaa gacggtatca aaggctttca
    24841 gtgccggaag ttgttcaata cctaacggta acaaatgcgc gcgctgatca ttacccagta
    24901 gtttacgcac cgcttcaaac tggcacagaa acagttgcgt agggtcgatc cccaccgcga
    24961 ggtgcgcgcc tgcgccaatc atgcgccaca tgtgataacc gctgccgcag ccgacatcca
    25021 gaatggtgcg tccggttaaa tcagaaagat ggggcagaac acgatcccat ttccagtcgg
    25081 aacgccattc ggtatcgatg ttgacgccat acagtgagaa cggccctttg cgccacggca
    25141 tcaggttgcg catcagcgtt tcaatgcgtt taatttgccc tgcgctcagt ggctcttcgc
    25201 tttctgccgt aacgctatgc aataaatcaa gacgatacgg tttaatttca ggtagaaact
    25261 ccaccgcatt tgaccactgc ttaaacaacc cgtgctgctg ctcgcgctgc cagttagcaa
    25321 tctgcgcggg cagcgtttcg agccagtgtg aaagatgatt tttggcaatc agagaataaa
    25381 agttaccaaa gtcgatcatg cagcgtcctc tgcttttaat gccaccagtg aaccaaagtt
    25441 aaagcactgg aaccacagct cactatgctc aaaaccggcg ttatgcaggc gtgctttatg
    25501 ggtttccacg gaatcggtca gcatcacgtt ttccagcatg ctgcgcttct ggctgatctc
    25561 cagttcgctg taaccgttgg cacgtttaaa gtcgtggtgc atgttgaaca acagttcacc
    25621 aactttggca tcttcgaaac tgaatttttc cgaaagcacc agcgcgccgc cggggttcag
    25681 cccttgataa attttatcca gtaacgcctg gcgctcggaa ggttccagga attgcagggt
    25741 aaaattcagc accaccatcg atgcgttttc aatggcgata tcgcgaatat caccttcaat
    25801 gacgtctact ggcgtagggg ctttataggc gtcaatatga cgacggcagc gttcaatcat
    25861 cgccggggag ttgtcgatgg caataatttt gcaattatca tgatgaatgt tgcgacgcac
    25921 cgagagcgtc gccgcgccca gagaacaacc cagatcgtaa acctgcgtac caggttgaac
    25981 gaagcgctcg gctaacatac caatcatgga aataatattg gaatagccgg gtacggaacg
    26041 ctggatcata tccgggaaga cttcagctac ccgttcatca aaggtccagt cgcccagtct
    26101 ggcgataggg gcagaaaata gcgtgtcgcg gtgagacata acgtaaaaat ccgggaaaaa
    26161 gaaagtggcg tattgtgcgc taacgcaggg agaaaaccaa ctcccagggc atataccaaa
    26221 gattcgccag caccatcagc aacagcgcac accaggtggc gctcatgccg gaacgtcgcc
    26281 agcggaacag acggtgatga aaaccgtaat aatgcatcag acgaccagca agcaaaacga
    26341 tgccgcaaat atgcaccatc caggtttctg cgccattcat ttccataaac agcatcagca
    26401 caatcgcgat gggaatatat tccaccgcgt taccatgaat gcgaatagcg ctttgcagtt
    26461 cgctaaaacc gccgtcgcca taggcaacgc ggtactgcat tcgcaggcga acgacatcaa
    26521 aagagaactt cattaataac aacgcactta aaacggcgta cagcgcgctt accatacaaa
    26581 ctccctttaa aatggccgat ggcacctgtc tatgataggt ggcaaattca gaaaagagaa
    26641 gattgctgtg gaatagccgg aactgctccg atctctggaa gcgttttacg taagtcttcc
    26701 cacagggtat gtaccagttc cggtgcctgg gcaatgtctg gcgtatgtaa aaaaagataa
    26761 ggcgtagtgg tctgatgcca ctgcgctaat ttttgtaacc agacctgaaa taattcccgg
    26821 ttttgcgtca tatcatcact accgataaaa cggatcagcg gatttgtcgc cgtcagtaca
    26881 gcatgtaccg gaactttagg tttttttcgt tgagcgtcgc gaatagcttc actgtgtgga
    26941 cgtgctgcat gaaccgggcg gctgtctaaa atcacccgat taacgccgcg ctgatgtaaa
    27001 ccgcgattaa gcgtttgttc ctcttctcct ttggcgaaaa actgtggatg gcggacttcc
    27061 acgccataat taaattcgcc aggaagagaa tcgagaaaat gccaaagcgc aggcagctcc
    27121 cgtgggccga atgtggcagg cagttgcagc cagtattgcc caatgcgcgg agccaacggt
    27181 gacatgcggg tcaaaaattc agtcactaaa tcatcgcaat gccgtaatgc tgcctgatgc
    27241 gaaatggtcg ccggaaactt aaaacagaag cggaagtcat ctgtggtctg ctcacgccag
    27301 cgcaggacaa cctcgggttt cggcagggcg taaagcgtgg tgttgccctc cacgcagtta
    27361 aagtggcggg catactcttc aaggctggtg atccccaacc gcacccattt aggatgcgac
    27421 cattgcggta gaccgatgta aatcataacg cgttgaggat ctcttccacg ctacgcacac
    27481 gggcgatgcg cgggtagata tggttgatac tgttattgtg ctgctcggcg ctggcggcac
    27541 tacaggcgtc ttcggcaatc accagattaa agccaagttc ccaggcattg cgggcggtgg
    27601 attcaacacc gatattggtc gagatcccac ataacactat tgtatcgata cctcggcggc
    27661 gtaattgcaa ctccagatcc gtaccgtaaa acgcacccca ttgacgtttg atgatttcga
    27721 tatcgctgtc ggttgcacct aatgcagcag gatgttgcca ccaattttcg ggcaacactt
    27781 tagccggtga gggcgcatcg accggctgtt ttaatgcttc agcgtaatcg gcagaccagc
    27841 caacgcgcac cagaaacacg ggctgaccgc tggcgcgaaa tttcgccgcc agcttcccgg
    27901 cgcgattaac tacctcatcg gcagtatgtg gaccaccggc aaaaggtaag atgccttctt
    27961 gtaaatcgat caccaccagc gcagtggttt tagcattaag ttcaagcata atgactcctg
    28021 aaaaaacaaa atcgtgcctc acaccttaat gcgattagcc gatggcggaa cgacaaattt
    28081 tgttaatttt tatgagttcg ggcaacacct ttatagcaaa ctggcgtaga gtctgatttc
    28141 aggcccgata taaagtggta acgaggcgtt ccagcgcggg ccaggctctt acggtgcggc
    28201 agaatttcca gtataatagc cgcctttttt cattcagttg tgacatacag ctaacgctgc
    28261 gacttggtca cctgcgcagc agggggcatc cgccggcagc taagttaagg gatatctcat
    28321 gcgtacagaa tattgtggac agctccgttt gtcccacgtg gggcagcagg tgactctgtg
    28381 tggttgggtc aaccgtcgtc gtgatcttgg tagcctgatc ttcatcgata tgcgcgaccg
    28441 cgaaggtatc gtgcaggtat ttttcgatcc ggatcgtgcg gacgcgttaa agctggcctc
    28501 tgaactgcgt aatgagttct gcattcaggt cacgggcacc gtacgtgcgc gtgacgaaaa
    28561 aaatattaac cgcgatatgg cgaccggcga aatcgaagtg ctggcgtcct cgctgactat
    28621 catcaaccgc gcagatgttc tgccgcttga ctctaaccac gtcaacaccg aagaagcgcg
    28681 tctgaaatac cgctacctcg acctgcgtcg tccggaaatg gctcagcgcc tgaaaacccg
    28741 cgctaaaatc accagcctgg tgcgccgttt tatggatgac cacggcttcc tcgacatcga
    28801 aactccgatg ctgaccaaag ccacgccgga aggcgcgcgt gactacctgg tgccttctcg
    28861 tgtgcacaaa ggtaaattct acgcactgcc gcaatccccg cagttgttca aacagctgct
    28921 gatgatgtcc ggttttgacc gttactatca gatcgttaaa tgcttccgtg acgaagacct
    28981 gcgtgctgac cgtcagcctg aatttactca gatcgatgtg gaaacttctt tcatgaccgc
    29041 gccgcaagtg cgtgaagtga tggaagcgct ggtgcgtcat ctgtggctgg aagtgaaggg
    29101 tgtggatctg ggcgatttcc cggtaatgac ctttgcggaa gcagaacgcc gttatggttc
    29161 tgataaaccg gatctgcgta acccgatgga actgactgac gttgctgatc tgctgaaatc
    29221 tgttgagttt gctgtatttg caggtccggc gaacgatccg aaaggtcgcg tagcggctct
    29281 gcgcgttccg ggcggcgcat cgctgacccg taagcagatc gacgaatacg gtaacttcgt
    29341 taaaatctac ggcgcgaaag gtctggctta catcaaagtt aacgaacgcg cgaaaggtct
    29401 ggaaggtatc aacagcccgg tagcgaagtt ccttaatgca gaaatcatcg aagacatcct
    29461 ggatcgtact gccgcgcaag atggcgatat gattttcttc ggtgccgaca acaagaaaat
    29521 tgttgccgac gcgatgggtg cactgcgcct gaaagtgggt aaagaccttg gtctgaccga
    29581 cgaaagcaaa tgggcaccgc tgtgggttat cgacttcccg atgtttgaag acgacggtga
    29641 aggcggcctg acggcaatgc accatccgtt cacctcaccg aaagatatga cggctgcaga
    29701 actgaaagct gcaccggaaa atgcggtggc gaacgcttac gatatggtca tcaatggtta
    29761 cgaagtgggc ggtggttcag tacgtatcca taatggtgat atgcagcaga cggtgtttgg
    29821 tattctgggt atcaacgaag aggaacagcg cgagaaattc ggcttcctgc tcgacgctct
    29881 gaaatacggt actccgccgc acgcaggtct ggcattcggt cttgaccgtc tgaccatgct
    29941 gctgaccggc accgacaata tccgtgacgt tatcgccttc ccgaaaacca cggcggcagc
    30001 gtgtctgatg actgaagcac cgagctttgc taacccgact gcactggctg agctgagcat
    30061 tcaggttgtg aagaaggctg agaataactg atatgactca aatacacgaa atcattcgcg
    30121 ttgcatcgag gcggcaactg agtgaactcc catgagcata gataactatg tgaatgggat
    30181 gagcgaaggc agtcaacgaa gaggcagcgt gaaggataaa gtgtataagc gtcccgtttc
    30241 gatcttagtg gtcatctacg cacaagatac gaaacgggtg ctgatgttgc agcggcgtga
    30301 cgatcccgat ttctggcagt cggtaaccgg cagcgtggaa gagggtgaaa ccgcgccgca
    30361 agctgccatg cgcgaagtaa aggaagaggt caccattgat gttgtcgctg aacaactgac
    30421 cttaattgac tgtcagcgca cggtagagtt tgaaattttt tcacatttac gtcatcgcta
    30481 tgcgccgggc gtgacgcgta atacggaatc atggttctgt cttgcgcttc cgcacgagcg
    30541 gcagatcgtt ttcactgaac atctggctta caagtggctt gatgcgcctg ctgcggcggc
    30601 gctcactaag tcctggagca accggcaggc gattgaacag tttgtaatta acgctgcctg
    30661 aacaggcagg gctatctgga gatattttta tggcaggtca tagtaaatgg gccaacacca
    30721 gacatcgtaa agctgcgcag gatgctaagc gcggtaaaat cttcactaaa atcattcgtg
    30781 agctggtaac cgcggctaag ctgggcggtg gcgatccgga cgctaacccg cgtctgcgtg
    30841 cggcggtgga taaagcactg tctaacaaca tgacccgtga cacactgaac cgcgcaattg
    30901 cacgtggtgt gggcggtgat gatgatgcaa acatggaaac catcatctac gaaggttacg
    30961 gtcctggcgg cacggcaatc atgattgaat gtctgtctga caaccgcaac cgtaccgttg
    31021 ctgaagtgcg tcatgcattt agcaaatgtg gcggtaacct cggtactgat ggttccgttg
    31081 cctatctgtt cagcaaaaaa ggcgtgatct ccttcgagaa aggcgatgaa gacaccatca
    31141 tggaagcagc actggaagca ggtgctgaag acgttgtgac ctatgatgac ggcgcgattg
    31201 atgtctacac tgcatgggaa gaaatgggta aagtgcgcga cgctctggaa gcggcaggtc
    31261 tgaaagcaga cagcgcggaa gtttccatga tcccgtctac caaagctgat atggatgcag
    31321 aaaccgcacc gaaactgatg cgtctgatcg atatgctgga agattgcgac gacgtgcagg
    31381 aagtttacca taacggtgaa atctctgatg aggtcgcagc gactctctga tgaggcctgc
    31441 taaacagcaa aacggagacg cgtgatggct attattctcg gcattgatcc gggttcgcgc
    31501 gtgaccggct acggcgtcat ccgccaggta ggtaggcaac tgtcctacct gggtagcgga
    31561 tgcatccgca ccaaagtgga tgatttaccg tctcgtctga aactcatcta tgcgggcgtg
    31621 acggaaatca tcacccagtt ccagcctgat tatttcgcca ttgaacaagt ctttatggca
    31681 aagaacgctg actcagccct gaaactgggc caggcgcgcg gcgtggcgat tgtggcggcg
    31741 gtgaatcagg agttgccagt atttgaatac gcggcacgtc aggtaaagca aacggtggta
    31801 ggtattggca gtgccgaaaa aagccaggtg cagcatatgg tccgcacctt gctgaaactg
    31861 cccgctaatc cacaggcgga tgccgccgat gcgctggcga ttgctatcac ccactgccac
    31921 gttagtcaga atgcgatgca gatgagcgaa tcgcggctga acctggcgag agggcgactg
    31981 cgttaagtta taccgcctcg gtcagttccg gctgaggcgt ttccactccc tccgcatgaa
    32041 tcaacaccaa acccggatga tgccacaaac tggctggcgt gacggtttta cgctcccacg
    32101 gaatagaacc caggaaccag aatttccaga aagtgagttt tgcattcgga ttgctattta
    32161 acaattctcc aaacgtctct atttcaccca ccggaataca tagcgcttct ttataaatat
    32221 caaaaacaaa ttttgaacag aactggcgcg aagattcgta tttaaaaccg gtgtggtaaa
    32281 gtttgcgtag ccgggaggga acctgttcaa caattcgttg tttttgtcgt tctgtcagcc
    32341 cagcgtctaa tcgctttata gcatagcgtt gattagacga acgtttaata aaacgagata
    32401 gcgtggtgat ggttgaaagg ggaacccggc tttctgcaac caggaagtct tcaccgttat
    32461 gaccgataat gatcccgacg tgattactcc agcaatttga tgcagctgat atttgaccaa
    32521 ataaggcagc acctatacat gtaaagacga tatctccaat ttcatattca gcagggtaat
    32581 taatattcac gttgtcattc cattgaaata gatacaaata tacgtaatag cataaaaaat
    32641 ttattttttc aggaggcgca atgtatattt ttattacgca tttcttcact gaatatgtaa
    32701 tattaaaata tttgcttcca atataacctg tagaataaat tatactgtgc catttttcag
    32761 ttcatcgaga cacctcgcaa gttttcttca tccttcgctg gatatctatc cagcattttt
    32821 ttatcataca gcattatctt tgattcatta cgcaggagcg tcatgtgata ggcagactca
    32881 gaggcatcat cattgaaaaa caacccccgc tggtgttaat tgaagtgggc ggcgtaggct
    32941 atgaagtgca tatgccgatg acctgttttt atgaactccc tgaagcgggt caggaagcga
    33001 tcgttttcac ccactttgtg gtgcgtgaag acgcgcaact gctgtacggt tttaacaata
    33061 aacaagagcg cacattgttc aaagagttga tcaaaaccaa cggcgtcggc ccgaagttgg
    33121 cgctggcgat cctctccgga atgtcagcgc agcagttcgt taatgccgtt gagcgtgaag
    33181 aagtgggggc actggtgaaa ctgccgggta ttggcaaaaa aaccgccgaa cgcttgattg
    33241 ttgaaatgaa agaccgattt aaaggtttgc atggcgatct ctttacgcca gccgccgacc
    33301 tggtactcac gtctcctgcc agcccggcga ccgacgatgc tgaacaagaa gcggttgccg
    33361 cgctggtggc gctgggctat aaaccacaag aagcaagccg catggtgagc aaaatcgctc
    33421 gccctgacgc cagcagtgaa actttaattc gcgaagccct acgcgccgcg ttatgaggta
    33481 aaggatgatt gaagcagacc gtctgatttc tgccggtacc actttgccgg aagatgtagc
    33541 agatcgcgcc attcgcccca aattactgga agagtatgtt ggtcagccgc aggttcgttc
    33601 acagatggag attttcatca aagcagcgaa actgcgcggc gatgccctcg atcatttgtt
    33661 gatttttggt cctccggggt tgggtaaaac tacgcttgcc aacattgtcg ccaatgaaat
    33721 gggcgttaat ttacgcacga cttctggtcc ggtgctggaa aaggcgggcg atttggctgc
    33781 gatgctcact aaccttgaac cgcatgacgt gctgtttatt gatgagatcc accgtctatc
    33841 gccagttgtt gaagaagtgc tgtacccggc aatggaagac taccaactgg atatcatgat
    33901 tggtgaaggt ccggcggcac gctccattaa aattgatttg ccgccgttta ccctgattgg
    33961 tgcaaccacg cgcgcaggtt cgctgacatc accgttgcgc gaccgttttg gtattgtgca
    34021 acgtctggag ttttatcagg tgccggatct gcaatatatc gtcagtcgca gcgcacgctt
    34081 tatggggctt gagatgagtg atgacggcgc gctggaagtt gctcgtcgcg ctcgcggtac
    34141 gccgcgcatt gccaaccgtc tgctgcgtcg agtgcgtgat ttcgccgaag tgaagcacga
    34201 tggcaccatc tcggcagata tcgctgctca ggcgctggat atgttgaatg tcgatgctga
    34261 aggtttcgat tatatggacc gcaaattgtt gctggcggta atcgataagt tctttggtgg
    34321 acctgtaggt ctggataacc tggcggcagc cattggcgaa gaacgtgaaa ccattgagga
    34381 tgtgctggaa ccttatttga ttcagcaagg ctttttgcag cgtacaccgc gtgggcgtat
    34441 ggcgacgacg cgggcgtgga atcactttgg cataacgccg ccagaaatgc cgtaagtcgg
    34501 attggtcgct cgcgccgcat ccggcactgg caatatgcct gatgcgacgc tgttcgcgtc
    34561 ttaccaggac taccagagat accgaaccgt aggtcggata aggcgctcgc gccgcatccg
    34621 acaaatgtgt tcagcgatag attagctggc ctgctttttc atcatactga gaataaataa
    34681 cagtgccgca catagcacca ccgacggacc cgccggcgta tcgtaaaccg cggaaaaggt
    34741 taaaccgcca gtcactgcca ccatccccac caaaacagcg acaccagcca tctgttccgg
    34801 cgtgcgggca aagcgacgcg cagtagcagc aggaataatc agcaacgaag taataatcaa
    34861 cgcaccgaca aatttcatcg ctacaccaat cgtcaatgcc gtcaccagca tcaacaacaa
    34921 tttcactcgc tgtaatttca caccatcaac aaacgccaga tccgggctta tcgtcattga
    34981 cagcaaattg cgccattgcc agaacaaaat agccaccacg atgaccacgc caatcgcaat
    35041 agagatgaga tcttctggcg tcactgccag caaatcaccg aacaggtaag ccatcaaatc
    35101 aacacgaata ttagacatca gactcacgac caccaggccc agcgacaggg cactgtgcgc
    35161 cataatccct aataacgtgt cgatcgccag ctgtggacgc ttctccagcc ataccagacc
    35221 gcccgccagc agcagcgtaa cggcaatcac cgcatagaat ggattcacgt ccagcaacaa
    35281 accaaacgcg acgccaagta atgaggcatg agccagcgta tcaccgaaat aagacatacg
    35341 acgccagact acaaacgaac ccagcggacc cgcggcacag gcgagcatga tcccggctaa
    35401 ccaaccggga aataataatt caatcatgag cgatcatttc cccgacgcaa aacaattcgt
    35461 ccctgtaaat cgtgacgatg gttatgatga tggcgataga tacccagttg ttcagcacca
    35521 cgaggaccaa acattgaaat aaactccgga tgcagggaaa caacttccgg tgtgccggaa
    35581 caacaaatgt ggtgattcag gcacagcact tcatcggttt ttgccatcac cagatgcaga
    35641 tcgtgagaaa ccattaaaac gccacaatcc agttcacgac gcagttggtc aataaggtca
    35701 tataacgcca cctggccatt tacatccacg ccttgagtgg gttcatccag cactaataat
    35761 tgcgggcgat ttaacaatgc tcgcgctaat agtacacgct gcgtttcgcc accagagagc
    35821 ttttgcatcg gtgcgttaat caggtgcccg gcctggacac gtttcagtgc aggcaaaata
    35881 tcttctttat gtgtaccagg gcgtaagcgt aaaaaacggt ttacggtcag tggcaacgtg
    35941 gtgtcgagat acagcttctg cggtacatag ccgatgcgca gttttccgtt gcgcttgata
    36001 accccttcat cgggtgttac cagcccgagc actacccgta ccagtgtcga cttacctgcg
    36061 ccatttggcc caagtaaagt caaaattttt ccaggtttaa gttccagcga cacatcagag
    36121 aggacgcggc gttggccaaa agaaaccgag acattttcca gggaaaccag acttgtcatg
    36181 ttaattttag tcttgcagta gtcatgaaat gttataatat cacacttctc atattcatta
    36241 cgattattgg tcgcattatg ttacataaaa aaacgcttct tttcgcagca ttatccgccg
    36301 ctctctgggg aggtgcaaca caggccgcag atgccgccgt tgtcgcttcg cttaaacccg
    36361 ttgggttcat cgcttctgcc attgctgatg gggtaacaga aacagaggtt ttacttcctg
    36421 acggcgcttc agaacatgat tattcactgc gcccatcgga tgtaaaacgc ttacagaacg
    36481 cggacttagt cgtttgggtt ggcccggaga tggaagcgtt tatgcaaaaa ccggtaagca
    36541 aattaccagg agcgaagcag gtaacgattg cgcagcttga agatgtgaaa ccgctactga
    36601 tgaaaagtat tcacggcgat gatgatgatc acgaccacgc ggaaaaaagt gacgaagatc
    36661 accatcacgg cgatttcaac atgcatcttt ggctttcccc agagatagcg cgggctacag
    36721 cggttgcaat ccatggaaaa ttagtggaac ttatgccgca aagtcgagcc aaacttgacg
    36781 ccaacctgaa ggattttgag gcacaattag cctcaaccga aacgcaggtt ggtaacgagc
    36841 tcgcgccgct caaggggaaa ggttatttcg tttttcacga tgcttacggc tatttcgaaa
    36901 aacagtttgg actgacaccg cttggtcatt ttaccgttaa ccctgagatt caacctggcg
    36961 cgcagcgttt acatgaaata agaacacagt tggttgagca aaaagcaacc tgcgtttttg
    37021 ctgagccaca gttcaggcca gcggtcgttg agagcgtcgc acgagggaca tccgttcgta
    37081 tgggaacgtt ggatcccctg gggacgaata tcaaactggg taaaacaagc tattcagaat
    37141 tcctgagtca attagccaac cagtatgcga gctgcctgaa aggagattaa tgaggaagtg
    37201 attacgtgca acagatagcc cgctctgtcg ccctggcgtt taataattta ccgcgaccac
    37261 accgcgttat gttggggtcg ctcaccgttc ttactctggc cgtcgctgtc tggcggcctt
    37321 atgtttatca ccgtgatgcc acgccaattg tcaaaaccat tgagctggaa cagaacgaaa
    37381 ttcgttcgct cttacctgaa gccagtgagc cgattgatca agctgcacaa gaagatgaag
    37441 ccattcccca ggacgaactg gatgacaaaa tcgccggtga agcgggcgtg catgaatatg
    37501 ttgtttccac tggcgatacg ctaagcagca ttctcaatca gtatggtatt gatatgggtg
    37561 atatcaccca actggctgcg gccgacaaag aattgcgtaa cctgaaaatc ggtcaacaac
    37621 tctcctggac attaaccgcg gacggcgaac tgcagcgcct cacctgggaa gtgtctcgtc
    37681 gtgaaacccg aacctatgac cgtactgccg ctaacggttt taaaatgacc agcgaaatgc
    37741 agcaaggaga gtgggttaac aatctgctga aaggtaccgt cgggggaagc tttgttgcca
    37801 gcgccagaaa cgccggttta accagcgccg aagtgagcgc agtgattaaa gccatgcagt
    37861 ggcaaatgga tttccgcaaa ctgaaaaaag gcgatgaatt tgcggtgtta atgtctcgag
    37921 aaatgcttga tggtaaacgt gagcaaagcc agctgctggg cgtacgtttg cgttcagaag
    37981 gtaaagatta ttacgctatc cgcgctgaag atggcaaatt ctacgatcgt aacggtactg
    38041 gtctggcgaa aggattcttg cgattcccga cggcgaaaca gttccgtatc tcgtctaact
    38101 ttaacccgcg tcgtactaat ccggtgaccg gtcgcgttgc accacacaga ggtgttgatt
    38161 tcgccatgcc gcagggtacg ccagtgcttt cagtgggtga cggtgaagtg gtggttgcca
    38221 aacgcagtgg cgcagcaggt tattatgtgg ctattcgtca tggtcgcagc tacaccacgc
    38281 gttatatgca cttgcgcaag attctggtga aaccgggaca gaaggtgaaa cgtggcgacc
    38341 gtatcgcgct ttccggtaat accggacgtt caaccgggcc gcatctgcac tatgaagtat
    38401 ggataaacca gcaggccgta aacccgctga cggcaaaact gccgcgtacc gaagggctga
    38461 ccggctccga tcgtcgcgaa ttcctggcgc aggccaaaga gattgtgccg cagctacggt
    38521 ttgattaatt aacatccatt cgcagccggt acgcagtcag taccggcttt ttttatttgg
    38581 tgcggggcaa gttgcgccgc tacactatca ccagattgat ttttgcctta tccgaaactg
    38641 gaaaagcatg gaaacgaaaa aaaataatag cgaacacatt cctgagtttg ataaatcctt
    38701 tcgccacccg cgctactggg gagcatggct gggcgtagca gcgatggcgg gtatcgcttt
    38761 aacgccgcca aagttccgtg atcccattct ggcacggctg ggacgttttg ccggacgact
    38821 gggaaaaagc tcacgccgtc gtgcgttaat caatctgtcg ctctgctttc cagaacgtag
    38881 tgaagctgaa cgcgaagcga ttgttgatga gatgtttgcc accgcgccgc aagcgatggc
    38941 aatgatggct gagttggcaa tacgcgggcc ggagaaaatt cagccgcgcg ttgactggca
    39001 agggctggag atcatcgaag agatgcggcg taataacgag aaagttatct ttctggtgcc
    39061 gcacggttgg gccgtcgata ttcctgccat gctgatggcc tcgcaagggc agaaaatggc
    39121 agcgatgttc cataatcagg gcaacccggt ttttgattat gtctggaaca cggtgcgtcg
    39181 tcgctttggc ggtcgtctgc atgcgagaaa tgacggtatt aaaccattca tccagtcggt
    39241 acgtcagggg tactggggat attatttacc cgatcaggat catggcccag agcacagcga
    39301 atttgtggat ttctttgcca cctataaagc gacgttgccc gcgattggtc gtttgatgaa
    39361 agtgtgccgt gcgcgcgttg taccgctgtt tccgatttat gatggcaaga cgcatcgtct
    39421 gacgattcag gtgcgcccac cgatggatga tctgttagag gcggatgatc atacgattgc
    39481 gcggcggatg aatgaagaag tcgagatttt tgttggtccg cgaccagaac aatacacctg
    39541 gatactaaaa ttgctgaaaa ctcgcaaacc gggcgaaatc cagccgtata agcgcaaaga
    39601 tctttatccc atcaaataaa aaagcctctc gcgaggagag gccttcgcct gatgataagt
    39661 tcaagtttgc ttcagaatat tcgaaatctg ttgaactatc attgaactgt aggccggatg
    39721 tggcgttttc gccgcatccg gcaacgtact tactctaccg ttaaaatacg cgtggtatta
    39781 gtagaaccca cggtactcat cacgtcgccc tgggtgacaa tcaccaggtc accagacatc
    39841 aagtaacctt tatcgcgcag cagattaacc gcttcgctgg cagctgctac gccgtcatta
    39901 gcgctatcaa agtgcaccgg cgtaacgcca cgatagagag cagtcaggtt cagcgtacgt
    39961 tcatggcgcg acatggcgaa aattggcaga ccagagctga tacgggaggt catcagcgcg
    40021 gtacgacccg attcggtcat ggtgatgatc gccgtaacgc ctttcaggtg gttagctgcg
    40081 tacattgctg acatggcaat agcttcttcc acattgtcga actgaacgtc cagacggtgt
    40141 ttagaaacgt tgatgctcgg gattttttcc gcacccaggc aaacgcgcgc catggctgca
    40201 acggtttctg acggatactg cccagcggca gtttctgcag acagcatcac agcgtcagta
    40261 ccatccagaa cggcgtttgc tacgtccatg acttctgcac gcgtcggcat cgggttagta
    40321 atcattgact ccatcatctg ggtcgccgtg attaccgctc ggtttagctg acgcgcacga
    40381 cggatcaacg ctttctgaat gccgaccagt tccgggtcgc caatttccac accgaggtcg
    40441 ccacgtgcaa ccattaccac gtcagaggcg aggatgatgt catccattgc atcctggctg
    40501 caaacggctt ccgcacgttc aaccttggca acaattttcg catcacatcc tgcatcgcgt
    40561 gccaggcgac gggcatagtt cagatcttcg ccacagcgtg ggaaggagac agccaggtaa
    40621 tctacgccaa tcaacgccgc agtcttaatg tctgctttgt ctttttcggt cagcgcttca
    40681 gccgacaaac cgccgccaag tttgttgata cctttattgt tggagagggg accaccgacg
    40741 gtgacttcgg tgaacacttt catgccctga acttccagta cttttaactg gacgcgacca
    40801 tcgtccagca gcaggatgtc accaggcacg acgtcagcag gcaggccttt gtagtcgata
    40861 ccgacttttt ctttgtcgcc ttcaccttta cccaggttgg cgtcgagcag gaatttatcc
    40921 ccaatattga ggaaaacttt gccttcttta aaggtggata cacggatttt gggcccctgg
    40981 aggtcaccca gaatagccac atgacgcccc agttttgcgg caatctcacg aactttatcc
    41041 gcgcgcattt tgtgatcttc aggcgagccg tgagaaaagt tcatacgtac aacgttggca
    41101 cccgccgcga taactttttc aagattatta tcgcgatctg ttgctgggcc taacgtggta
    41161 acgatttttg ttctgcgaag ccttctggac atgtaatact ccgttgactg aaacaaccag
    41221 gtgttgcttg aacatgaaat ccgaatgaaa taacgccgcg atggcgataa attaatctca
    41281 ttcggatgtc gtataagatt aggacagtga cagtcgtttt tagcgatcgt cacttaaatt
    41341 aagtaactgc ttatcaaaac gcgattcctt cagcgcttct ttgacccgct tcaagttatc
    41401 tctgaatttt gcaccgcgtc gcaaagtaaa tcctgtcgcc agcacatcta tcacggtcag
    41461 ctgtgcaagt cgagaaacca tgggcatata aatgtcagta tcttccggta cgtcgagggt
    41521 aattgccagc gttgcttccc gggcgagcgg ggtacccgca gaggtgaggg caatcaccat
    41581 ggcgtcgttt tcgcgtgcca gctgcgccag ctcgaccaga ttttttgttc ttccagtgtg
    41641 agaaatcagc accaccacgt ctccgtcgct acaattcata caactcatgc gttgcagcac
    41701 gatatcatcg gagtacacca ccggaacatt aaaacgaaag aacttattca tcgcatcgtg
    41761 ggcaacggcg gctgaagagc ctaatccgaa aaaggcgatt ttttttgcct gagtgagcaa
    41821 gtcgacggcg cggttgatgg cagatttatc cagtgaatga cggacatgat caagcgttgc
    41881 cattgcggac tcaaatattt tccctgtgta tgattcaacg ctgtcatctt cattgacatt
    41941 gcgattaaca tagggagtgc cattcgccag actctgtgcc agatgaagtt taaaatcagg
    42001 aaaaccgcgc gtgtccatgc tgcgacagaa acgattcacc gtcggttcgc taacattggc
    42061 ttccagtgcc atagcagcaa tactcgaatg gatcgcgtta tcgggcgaag ccagaatgac
    42121 ctcggcaact ttgcgctctg atttgctcaa atgttccagc tgagactgga ttttttccag
    42181 catattcatg atgtaaagag actcacgggt aatgacgatt tccgcactga aagaaatcga
    42241 aatgcagttt tgtcagatat tacgcctgtg tgccgtgtta atgacaaaag cagataaaaa
    42301 agttgttatt ttttttcata acatgatcag tgtcagattt ttacccaatg gaaaacgatg
    42361 atttttttat cagttttgcc gcactttgcg cgcttttccc gtaatcgcac gggtggataa
    42421 gcgtttacag ttttcgcaag ctcgtaaaag cagtacagtg caccgtaaga aaattacaag
    42481 tataccctgg cttaagtacc gggttagtta acttaaggag aatgacatgg cggtaacgca
    42541 aacagcccag gcctgtgacc tggtcatttt cggcgcgaaa ggcgaccttg cgcgtcgtaa
    42601 attgctgcct tccctgtatc aactggaaaa agccggtcag ctcaacccgg acacccggat
    42661 tatcggcgta gggcgtgctg actgggataa agcggcatat accaaagttg tccgcgaggc
    42721 gctcgaaact ttcatgaaag aaaccattga tgaaggttta tgggacaccc tgagtgcacg
    42781 tctggatttt tgtaatctcg atgtcaatga cactgctgca ttcagccgtc tcggcgcgat
    42841 gctggatcaa aaaaatcgta tcaccattaa ctactttgcc atgccgccca gcacttttgg
    42901 cgcaatttgc aaagggcttg gcgaggcaaa actgaatgct aaaccggcac gcgtagtcat
    42961 ggagaaaccg ctggggacgt cgctggcgac ctcgcaggaa atcaatgatc aggttggcga
    43021 atacttcgag gagtgccagg tttaccgtat cgaccactat cttggtaaag aaacggtgct
    43081 gaacctgttg gcgctgcgtt ttgctaactc cctgtttgtg aataactggg acaatcgcac
    43141 cattgatcat gttgagatta ccgtggcaga agaagtgggg atcgaagggc gctggggcta
    43201 ttttgataaa gccggtcaga tgcgcgacat gatccagaac cacctgctgc aaattctttg
    43261 catgattgcg atgtctccgc cgtctgacct gagcgcagac agcatccgcg atgaaaaagt
    43321 gaaagtactg aagtctctgc gccgcatcga ccgctccaac gtacgcgaaa aaaccgtacg
    43381 cgggcaatat actgcgggct tcgcccaggg caaaaaagtg ccgggatatc tggaagaaga
    43441 gggcgcgaac aagagcagca atacagaaac tttcgtggcg atccgcgtcg acattgataa
    43501 ctggcgctgg gccggtgtgc cattctacct gcgtactggt aaacgtctgc cgaccaaatg
    43561 ttctgaagtc gtggtctatt tcaaaacacc tgaactgaat ctgtttaaag aatcgtggca
    43621 ggatctgccg cagaataaac tgactatccg tctgcaacct gatgaaggcg tggatatcca
    43681 ggtactgaat aaagttcctg gccttgacca caaacataac ctgcaaatca ccaagctgga
    43741 tctgagctat tcagaaacct ttaatcagac gcatctggcg gatgcctatg aacgtttgct
    43801 gctggaaacc atgcgtggta ttcaggcact gtttgtacgt cgcgacgaag tggaagaagc
    43861 ctggaaatgg gtagactcca ttactgaggc gtgggcgatg gacaatgatg cgccgaaacc
    43921 gtatcaggcc ggaacctggg gacccgttgc ctcggtggcg atgattaccc gtgatggtcg
    43981 ttcctggaat gagtttgagt aatatctgcg cttatccttt atggttattt taccggtaac
    44041 atgatcttgc gcagattgta gaacaatttt tacactttca ggcctcgtgc ggattcaccc
    44101 acgaggcttt ttttattaca ctgactgaaa cgtttttgcc ctatgagctc cggttacagg
    44161 cgtttcagtc ataaatcctc tgaatgaaac gcgttgtgaa tcatcctgct ctgacaactc
    44221 aatttcagga gcctttatga atccacaatt gttacgcgta acaaatcgaa tcattgaacg
    44281 ttcgcgcgag actcgctctg cttatctcgc ccggatagaa caagcgaaaa cttcgaccgt
    44341 tcatcgttcg cagttggcat gcggtaacct ggcacacggt ttcgctgcct gccagccaga
    44401 agacaaagcc tctttgaaaa gcatgttgcg taacaatatc gccatcatca cctcctataa
    44461 cgacatgctc tccgcgcacc agccttatga acactatcca gaaatcattc gtaaagccct
    44521 gcatgaagcg aatgcggttg gtcaggttgc gggcggtgtt ccggcgatgt gtgatggtgt
    44581 cacccagggg caggatggaa tggaattgtc gctgctaagc cgcgaagtga tagcgatgtc
    44641 tgcggcggtg gggctgtccc ataacatgtt tgatggtgct ctgttcctcg gtgtgtgcga
    44701 caagattgtc ccgggtctga cgatggcagc cctgtcgttt ggtcatttgc ctgcggtgtt
    44761 tgtgccgtct ggaccgatgg caagcggttt gccaaataaa gaaaaagtgc gtattcgcca
    44821 gctttatgcc gaaggtaaag tggaccgcat ggccttactg gagtcagaag ccgcgtctta
    44881 ccatgcgccg ggaacatgta ctttctacgg tactgccaac accaaccaga tggtggtgga
    44941 gtttatgggg atgcagttgc caggctcttc ttttgttcat ccggattctc cgctgcgcga
    45001 tgctttgacc gccgcagctg cgcgtcaggt tacacgcatg accggtaatg gtaatgaatg
    45061 gatgccgatc ggtaagatga tcgatgagaa agtggtggtg aacggtatcg ttgcactgct
    45121 ggcgaccggt ggttccacta accacaccat gcacctggtg gcgatggcgc gcgcggccgg
    45181 tattcagatt aactgggatg acttctctga cctttctgat gttgtaccgc tgatggcacg
    45241 tctctacccg aacggtccgg ccgatattaa ccacttccag gcggcaggtg gcgtaccggt
    45301 tctggtgcgt gaactgctca aagcaggcct gctgcatgaa gatgtcaata cggtggcagg
    45361 ttttggtctg tctcgttata cccttgaacc atggctgaat aatggtgaac tggactggcg
    45421 ggaaggggcg gaaaaatcac tcgacagcaa tgtgatcgct tccttcgaac aacctttctc
    45481 tcatcatggt gggacaaaag tgttaagcgg taacctgggc cgtgcggtta tgaaaacctc
    45541 tgccgtgccg gttgagaacc aggtgattga agcgccagcg gttgtttttg aaagccagca
    45601 tgacgttatg ccggcctttg aagcgggttt gctggaccgc gattgtgtcg ttgttgtccg
    45661 tcatcagggg ccaaaagcga acggaatgcc agaattacat aaactcatgc cgccacttgg
    45721 tgtattattg gaccggtgtt tcaaaattgc gttagttacc gatggacgac tctccggcgc
    45781 ttcaggtaaa gtgccgtcag ctatccacgt aacaccagaa gcctacgatg gcgggctgct
    45841 ggcaaaagtg cgcgacgggg acatcattcg tgtgaatgga cagacaggcg aactgacgct
    45901 gctggtagac gaagcggaac tggctgctcg cgaaccgcac attcctgacc tgagcgcgtc
    45961 acgcgtggga acaggacgtg aattattcag cgccttgcgt gaaaaactgt ccggtgccga
    46021 acagggcgca acctgtatca ctttttaaga cgacaaattt gtaatcaggc gagagaaaac
    46081 tctgatgaaa aactggaaaa caagtgcaga atcaatcctg accaccggcc cggttgtacc
    46141 ggttatcgtg gtaaaaaaac tggaacacgc ggtgccgatg gcaaaagcgt tggttgctgg
    46201 tggggtgcgc gttctggaag tgactctgcg taccgagtgt gcagttgacg ctatccgtgc
    46261 tatcgccaaa gaagtgcctg aagcgattgt gggtgccggt acggtgctga atccacagca
    46321 gctggcagaa gtcactgaag cgggtgcaca gttcgcaatt agcccgggtc tgaccgagcc
    46381 gctgctgaaa gctgctaccg aagggactat tcctctgatt ccggggatca gcactgtttc
    46441 cgaactgatg ctgggtatgg actacggttt gaaagagttc aaattcttcc cggctgaagc
    46501 taacggcggc gtgaaagccc tgcaggcgat cgcgggtccg ttctcccagg tccgtttctg
    46561 cccgacgggt ggtatttctc cggctaacta ccgtgactac ctggcgctga aaagcgtgct
    46621 gtgcatcggt ggttcctggc tggttccggc agatgcgctg gaagcgggcg attacgaccg
    46681 cattactaag ctggcgcgtg aagctgtaga aggcgctaag ctgtaaaagt caaaatgccc
    46741 gatcgaggat cgggcatttt tgtagcgttt ttttgccggg tttaaccctg tacttttacc
    46801 tgtccggcgg cgtgcttcgc gcgttcaatg gcgtcaacaa cactctctgc agtagccagt
    46861 gccaccccca gacgacggct gccatcaatt tccggcttac caaataaacg aatctgcaaa
    46921 tctgcgccta cggcattctg cacattatca aacgtgacat tctgactggt cagttgtggc
    46981 agaataacgg cagaagctgc aggaccatac tgacggatcc cgccaaccgg aagtccgagg
    47041 aaggcacgta catgcagggc aaactctgag agatcttgag aaattaacgt caccatcccg
    47101 gtatcatgtg gacgagggga gacctcactg aaaatcacct catcaccaca gacaaatagc
    47161 tcgacaccaa acaacccata accgcccagt gccagcacca ctttacgggc aatctcctgc
    47221 gcacgttcaa gggcaagcgg gctcatttgc tgtggttgcc aggattcacg gtagtcgcca
    47281 tcttcctggc gatgacctac tggtgcacag aaatggacgc catccaccgc gctgacggtt
    47341 agcagggtaa tttcgaagtc aaacttaacg acgccttcaa caattacgcg gcccgctccg
    47401 gcgcgaccgc cttgctgagc gtacttccat gcctgagcaa gttgctctgc agaacgaata
    47461 aacgtctgcc ccttgccgga agagctcatc accggtttta caatgcaggg atagccaatg
    47521 tcagcaaccg cctcgcggaa aaggctttcg ctatcggcaa aacgataagt ggaagtgggc
    47581 agctgcagct cttctgccgc caggcgacgg ataccctcgc gattcatcgt taatttcgtt
    47641 gcgcgagcgc aggggacaac attcagtcct tcctcttcaa gttggatcag catatcggtg
    47701 gcaatagctt cgatctccgg cacgatataa tgtggttttt ccagttcaac cacacggcgt
    47761 aatgcatcac catcaagcat attaatgaca tgggagcgat gcgcgacatg catggctggt
    47821 gcgtcggcat agcgatcgac ggcaatcacc tctacgccga gacgctgaca ctcgattgcc
    47881 acttctttac ccagttcacc ggagcctaat aacatcacgc gagttgctgc cggacgcagc
    47941 gcagtgccta ataacgtcat aacgaatatc ccctgattaa ttccgcgcgc agtataaacg
    48001 aaaacgtttg cgtgtgtctt tattgctgat gttgatttct caaccgaaaa gaaatatact
    48061 gtataaaatc acagttatta tgagaggctg caaatggcgg ttgaagttaa atacgtagtc
    48121 attcgtgagg gcgaagagaa aatgtcgttt accagcaaaa aggaagccga tgcttatgac
    48181 aaaatgcttg atacggcgga tctgcttgac acctggctga caaattctcc agtgcaaatg
    48241 gaagacgagc aacgtgaagc cctttcgcta tggctggcag aacaaaaaga tgtgctgagc
    48301 accattctga aaaccggcaa attaccgtcc ccacaggtcg tcggtgctga atccgaagag
    48361 gaagatgcct cacacgcggc ctgaagcaac ttgcgctcct gacaatttgt accatgcttt
    48421 ctcctgaatc attgtgctat ggagaaaaac atgaaaaaaa gaggggcgtt tttagggctg
    48481 ttgttggttt ctgcctgcgc atcagttttc gctgccaata atgaaaccag caagtcggtc
    48541 actttcccaa agtgtgaagg tctggatgct gccggaattg ccgcgagcgt aaaacgtgat
    48601 tatcaacaaa atcgcgtggc gcgttgggca gatgatcaaa aaattgtcgg tcaggccgat
    48661 cccgtggctt gggtcagttt gcaggacatt cagggtaaag atgataaatg gtcagtaccg
    48721 ctaaccgtgc gtggtaaaag tgccgatatt cattaccagg tcagcgtgga ctgcaaagcg
    48781 ggaatggcgg aatatcagcg gcgttaatgg cttctgataa ttcgattgac tgttggcgga
    48841 tgcggcgcga gtgccttgtc cgccccagaa cgcaccacaa tcgtaggcct gagaagacgc
    48901 aacagcgtcg catcgggcaa tcagcatcaa acaatgccac tcaaaacgac aaactacttt
    48961 cacctaaaat acccttatca cctgatatac agtaaatacc ttacctggcg cgaatttctt
    49021 tgaataacgg cctaccgccc cgcttatttg cgcatctttt gtctcgatat acattttctg
    49081 acatcctcat ccgctatgct acaggcacta aaaatggtta ttgaggagca aatatggcta
    49141 actggttaaa tcaactgcaa tccctgcttg ggcaaagcag ttcttctacc tcctcgtctg
    49201 cggatcaggg attggtcaaa ctgttagtgc caggcgcatt aggcggactg gctgggctgc
    49261 tggtcgcaaa taaatcagca cgtaaacttc ttacgaaata tggcaccaac gcgttactgg
    49321 ttggcggcgg agcggtagcg ggtacggtgc tgtggaataa atacaaagat aaaattcgcg
    49381 cggcgcatca ggacgaaccg cagtttggcg cgcaaagtac gccgctggat gagcgtacag
    49441 cacgtttgat ccttgcgctg gtctttgccg ctaaaagtga tggtcatatt gatgccaaag
    49501 aacgtgcggc aatcgaccag caattgcgtg gagccggcgt ggaagagcag gggcgtgtac
    49561 tcattgagca ggcaatcgaa caaccgctgg atccacaacg cctggctacc ggggtccgca
    49621 atgaagaaga ggcgctggaa atctatttcc tgagttgcgc ggctattgat attgaccatt
    49681 ttatggaacg cagttacctg aatgcactgg gtgacgcgct gaaaatcccc caggatgttc
    49741 gtgacggcat tgaacgcgat ctcgaacagc aaaaacgcac tttggcggaa taattccgct
    49801 ctctggatag cctgaagttg actatttcgg gctatccaga atcgcacagc catttgaaca
    49861 ggctgatttt ctctgtctgc ataacattcg atatccgcgc cattacctgt catcatctaa
    49921 gcaatgactc ccctgtttcg cttgcatccc cggtgagttt tgccaccctt ataagatgtt
    49981 tcaaccagaa agaacaataa catgctacca aaagccgccc gcattcccca cgccatgacg
    50041 cttcatggcg atacgcgcat cgataattac tactggctgc gggacgatac gcgttctcag
    50101 ccagaagtcc tggactacct gcaacaagaa aatagttacg gtcatcgggt gatggcctca
    50161 caacaagcct tgcaggatcg catcttaaag gaaatcatcg accgcattcc gcaacgagaa
    50221 gtttctgcgc cctacatcaa aaatggctac cgctatcggc atatttatga accaggctgt
    50281 gaatatgcta tctaccagcg tcaatcggca ttcagtgaag agtgggatga gtgggaaaca
    50341 ttgctcgatg ccaataagcg cgcagctcat agtgagtttt attcgatggg cggaatggcg
    50401 attacgcccg ataacaccat tatggcgctg gcagaagatt ttctttcccg acgccagtac
    50461 ggcattcgtt ttcgtaatct ggaaactggt aactggtacc cggaactgct ggataacgtt
    50521 gaacccagct ttgtctgggc aaatgactcc tggattttct actatgttcg caagcatccg
    50581 gtgacgctgc tgccttatca ggtctggcgt cacgccatcg gtacgccagc atcgcaagat
    50641 aaactgatct acgaagaaaa agacgatacc tattacgtca gcctgcataa aacgacctcg
    50701 aagcactatg tagtcattca tttggccagc gccaccacca gtgaagttcg cctgctggac
    50761 gcggaaatgg ccgatgccga gccgtttgtt tttctgccgc gccgcaaaga tcacgaatac
    50821 agccttgatc actaccagca tcgtttttat ctgcgttcca accgccacgg caaaaacttt
    50881 ggcttatacc gtacccgtat gcgtgatgag caacagtggg aagagttaat tccgccacgc
    50941 gaaaacatca tgctggaagg gtttacgctg tttaccgact ggctggtggt tgaagagcgt
    51001 cagcgcgggt taaccagttt gcgccaaatt aaccgcaaga cccgggaagt cattggtatt
    51061 gcctttgatg atccggccta tgtgacctgg attgcctaca atccagaacc tgaaaccgcg
    51121 cgattgcgtt atggttattc ttccatgact acaccagaca ctttgtttga actggatatg
    51181 gataccggtg agcgtcgtgt attaaaacaa acggaagttc ctggttttta tgcggcgaat
    51241 taccgcagtg aacacctgtg gatagtcgcc cgtgatggcg tcgaagttcc ggtttcgttg
    51301 gtctaccatc gcaaacattt tcgcaaagga cacaacccgt tgctggtgta tggctatggt
    51361 tcttacggcg caagtattga tgccgatttc agttttagcc gcttgagttt gttagatcgt
    51421 ggctttgtct acgccattgt ccatgctcgc ggcggtggtg agctggggca acaatggtac
    51481 gaagacggaa aatttctgaa gaagaaaaat acgtttaatg attatcttga tgcctgcgat
    51541 gcattgttaa aactgggcta tggctctcct tcgctttgtt atgcgatggg cgggagtgcg
    51601 gggggcatgt tgatgggcgt tgcaattaat caacgcccgg aattattcca cggcgttatc
    51661 gcccaggtac cgtttgttga tgttgtaaca acgatgcttg atgaatcaat tcctcttacc
    51721 actggtgagt ttgaagagtg gggtaacccg caggagccgc aatattacga gtacatgaaa
    51781 agctacagcc cgtatgacaa cgtcaccgca caggcttatc cgcatttact ggtaacgacc
    51841 ggtttgcacg attctcaggt gcaatattgg gaaccggcaa aatgggtcgc taaattgcgc
    51901 gagctgaaaa ccgatgacca tcttttattg ctctgtaccg acatggactc aggccatggc
    51961 ggtaaatctg gtcgctttaa atcgtacgaa ggcgtagcga tggaatatgc ttttctggtc
    52021 gcgctggcgc agggaacatt acccgctacg cctgcggact aagtattttc cagataatgt
    52081 ttcagtgtta aacgcagctc cgggctcatg ctgtccaggt tattaaataa ccagcgcaga
    52141 tagcccggat cgcgttcggc aacgtcggaa accgctttgc cacggtattt gccaaaggtg
    52201 aaggtcgtca tcaacgacgg acgtccggtg atatcggcca tctgttctgc cgtccagccg
    52261 gaggtgttca taatatcgat aagcaacgcg gcggtgatat aacagtcata caacgcgcgg
    52321 tgatgatgca ggcccggcgg cgtctgtaca ttgagcttgc gtgttttata taacgccata
    52381 ttgctgtact tgatcccagg ccacaaacga cgggccagtt tcatagtgca aatccactca
    52441 ccgggcatct caggcagtac gcggcggtca aagctggcgt tatgcgcgac ataccattca
    52501 ctaccgtaat agtgtgggat cacatcttca atccacggtt tatcggcgac catggcttcg
    52561 gtgatgcgat gaatcgccat cgcttgtgga ctaataggac gatcggggcg caccaggtgg
    52621 ctcatggggt tgacgatttt tccgtcaatg acatcaacag aggcaatctc aacgatccct
    52681 ccctgcaaac cgcaggtttc tgtatcgata atgcgcaaca tggcatgctc caggccgaaa
    52741 aagcctagcg taatggaatg atatcgcctt gccaaccctg tgatgaacgc tgaccgacta
    52801 acaataatga accacgatcg gcgcgaacga tgagacgacc atattcatcc catagtgcac
    52861 tgttaccacg ggcatttgcc atcagcacgg caatcgagta ctgatgtgaa aaaaattgta
    52921 agcgacgggc cgacgccagc aggtcgggtt ctcccagaca ttgccctgta gtaaacagtg
    52981 aacaggtcgg gtccatatcc ataccttgcg gttgttcatc gactaccgtg atggttctgg
    53041 aacgacggcc cagacatgca ccatgactct gatggtagat cccaggcgtt tttcgccacg
    53101 gtgcgaacac cgcaattcca cgaataaagc gatcgttata ttcaacggga aggccggcaa
    53161 tgatgttcat tcgccaggtc gttgcggcat aacaaagcgg gtctaacagt gagagatcgg
    53221 gtggggcagg aagggcacgt cgcgaataat cacaccccag taaagaaagc gatggaaaaa
    53281 ctaacagctg acattgttgc ctggcggcgg ccctcacaaa ctccagatga tgagctacct
    53341 gttcggtgag tgaggtttta cgaggctcat attgcgctgc tgcaactttc cagaacgaca
    53401 taatgacttc cttttcaaaa tgactgcaaa ttatgagtgc ggtttagaaa atactcataa
    53461 tttagcaggc gttatgtaag aaagtgtaac tctgtataag ttttatttaa gtttgggctc
    53521 gtaaggtaaa cgtgacagat tgaccgaagc caaacggtgg gcaataagcc gctcgcgaaa
    53581 ccagctgcgc aaatgttcag gctgttcacg ttcaaccgct tcagcgatca ccggcatatt
    53641 gtagcgttct ttaaatgcca ccccggccgc cgccaaatcg acattcactt tatccatttc
    53701 tgtttgatcc agtttagcca gattcttcag cattcttaat ctcctcatca ttcgcgggca
    53761 aaagttacta agagtgagcc aggatggcaa gccggaattg atgatcctcg actcagacat
    53821 tagacaaggt tatgctctgt aaaaaagtta ttacaaaaag gaatagataa tatggcttca
    53881 actgcacgct cccttcgcta cgcgctggca atcctgacca cttcgttggt gacaccttcc
    53941 gtctgggcac atgcgcattt aacgcatcag tatcctgcgg caaacgcgca agtgacagct
    54001 gcaccgcagg caatcacttt aaacttctcg gaaggtgttg aaacaggatt cagtggtgca
    54061 aaaatcacgg ggccaaaaaa cgaaaatatt aaaacattac cggcgaagcg aaatgagcag
    54121 gaccaaaagc aactgattgt tccactggcc gattcgctga aacccggaac ctacaccgtt
    54181 gactggcatg ttgtttcggt ggacgggcat aaaacgaaag gacactacac ctttagcgtg
    54241 aaataaatca tgctggcgtt tacctggatc gcgctgcgat ttatccattt cacctcgctg
    54301 atgctggttt ttggcttcgc aatgtacggt gcctggctgg cccccttgac gattcgtcgc
    54361 ttgctggcga agcgttttct gcgtttacaa cagcacgctg ccgtctggag tttgatcagt
    54421 gccactgcaa tgctcgccgt tcagggcggg ttgatgggaa cgggatggac agatgtattt
    54481 tcgccaaaca tttggcaggc tgttttgcaa acgcagtttg gtggcatctg gttatggcaa
    54541 attgttctcg ccctcgtaac gttgatcgtc gcccttatgc aaccgcggaa tatgccacgt
    54601 ttgctgttta tgctcactac cgcgcaattt attctgctgg cgggagtggg gcatgcgacg
    54661 ctgaatgaag gggtaacagc gaaaatccat cagactaatc acgcgattca cctgatttgt
    54721 gcggcggcgt ggtttggggg attactgccg gtgctctggt gtatgcaact catcaaaggc
    54781 cgttggcgac accaggctat tcaggcgctg atgcgttttt cctggtgcgg gcattttgcg
    54841 gtgatcggcg tactggcaag cggcgtgctt aatgcattgt tgattaccgg atttcccccc
    54901 acgcttacga cttactgggg ccaactctta ttgctcaaag ccatcctggt gatgattatg
    54961 gtggtcattg cgctggctaa tcgttatgtt cttgtaccgc gcatgaggca ggatgaagat
    55021 cgtgcggcac cctggtttgt gtggatgact aaactcgaat gggcgatagg tgcggtagtg
    55081 ctggtgatca tcagcctgct tgcgactctc gaaccttttt gatggattac gaaaatgatg
    55141 aaaaaaagta ttctggcgtt tctgttactc accagttctg cagcggcgct ggctgcaccg
    55201 caggtgatta ccgtcagtcg ttttgaagtg ggtaaagaca aatgggcgtt taatcgcgaa
    55261 gaggtgatgc tgacttgccg accgggtaat gctttgtatg ttattaatcc gagtaccctc
    55321 gtgcagtatc ctttaaacga tatcgcacaa aaggaagttg ccagtgggaa gaccaatgcc
    55381 caacccattt cggtgattca gattgatgat cctaacaatc ccggcgaaaa aatgagtctg
    55441 gcaccgttta tagaacgagc tgaaaaactc tgttaattac ctaaaatagc cttttgattt
    55501 ccaataaaaa aaccgcctca gttctttcac cagaacgggc ggtttttaac atttcagctg
    55561 atgaccacca cgctttttat tgaccatttt gcacgcaaac tggaaaacct ggcgtcgtca
    55621 tctattctta aagggcaagg caactaagcc tgcattaatg ccaactttta gcgcacggct
    55681 ctctcccaag agccatttcc ctggaccgaa tacaggaatc gtgttcggtc tctttttatc
    55741 tgttaaaagc cagaagcatt tccttcgctg actttatagt caaccataac acacactcta
    55801 ctgtctgagt ccagcgtttt ttaacattct tgttaagatt atgtgatctt tagcgcggga
    55861 ggaaaatatt gatgaaacag cctgcgcccg tttatcagag aattgcgggt catcaatggc
    55921 gacatatctg gctttctggc gatatacacg gttgtcttga gcagttgtgc cgcaaattat
    55981 ggcattgtcg ttttgatccg tggcgagatt tacttatctc agtgggagac gttatcgatc
    56041 gtgggccgca aagtttacgt tgtctgcagt tactggaaca acattgggtt tgtgcggtaa
    56101 gaggcaatca tgaacagatg gcgatggatg cgctggcatc ccagcagatg tctttgtggt
    56161 tgatgaatgg cggcgactgg tttattgcgc tggcagataa tcaacagaaa caagcgaaaa
    56221 cggcgctgga aaaatgtcag catttgccct ttattcttga agtacacagt cgcaccggca
    56281 agcatgttat tgctcatgcc gattatccag atgatgttta tgaatggcaa aaggacgttg
    56341 atttgcatca ggtcttgtgg agccgctcgc gattaggtga acgccaaaaa gggcagggaa
    56401 ttacaggtgc tgatcatttc tggtttggtc atacaccgtt gcgacatcgc gtggatattg
    56461 gcaacctgca ttatattgat accggtgctg tctttggggg cgaactgact cttgtgcaat
    56521 tgcaataatt aaaaatcacc gtactcctgt gcaggtcgcc agaaaccatc tataaaatcc
    56581 tcaatcggaa aacaaccgcc ctggcggatc cgttgatcgc tcatagaata aagacactgc
    56641 tgttccgtgt tgtagacatc cacaacaata tcttcacaac cgccatccag gtagcaaaca
    56701 aaaagtacca gcgcgaacat ttcatccccg aagtgtggtg ccgtaccgtt aagtttagga
    56761 gagattttac aacgggggaa taaccaggac aaataacccg ccatgccgac gggttctttt
    56821 tggatcaggc aagacgcata atccaggcat cagattgttg atcgtaacgg gtacggtaaa
    56881 ggacagagtg ttcgccattt agcagggcag ggatactggt ttcagcatcc caggcatcca
    56941 gttgcataca taaatcgggg tcagatttac aaggaatggt taacgttcga tcacctggcg
    57001 attcaccgtg taattcgccg tcgtcgattt caaaagcgcc tatgcgcaca ctggttttca
    57061 tcttcgtgct ctccatttag ctgccgattg tggtacgacc agtccgatcg caccattttt
    57121 cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg cgcttttttt gtgcgctgtc
    57181 aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc acgcggatag ctgtttttca
    57241 atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg ctggaaagta accaatacat
    57301 catccgccac tggcgcggct tgcgggtaaa catcgcgccc gcgataccac tcctccgctt
    57361 cctgcggtgt cagctcgaat gcattcatat tatcgggtga ggcaagagca ataaccgctt
    57421 catgctgcca gcgataacct ttgttgtgcg tctcggcaag tttaatcccc aaccgagaaa
    57481 atcggacttt accgatcagg gcttcaatgc ccaccgggaa caaccacagt tctttgtcac
    57541 gctgccagag gcgcaggttt tcatcccagt ttaagccaac acctgtagcc gcctgacgaa
    57601 tttgtccagc ttcgcgatct ttcaccgggc tgaacggaaa attaccgact ttgtatttgg
    57661 gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg agcaacgaag aagccttcgc
    57721 agtcgtaaat ttgtgggaaa acatgcaaaa agccttcttc ggtcagcgct ttgtttgcac
    57781 cagggaagag atcgccaagt ggtaaaaact ctactgcgtc ggggtaagtc tctttcagcc
    57841 acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca ggtcgagtaa accagcgtac
    57901 caccaggacg taatgcatga aaggcgctgt cgataagctc ccgttgtgta gctgcgattt
    57961 cctgattgct ttctggtgac cagtttttta gcgcatcggg atctttacgc accacgcctt
    58021 cgccagagca gggagcgtcc agcaaaatgg catcgaacat ttctggcact gccgcaccaa
    58081 acacgcggcc atcaaaatgt gtgagcgcaa cattactgat accacagcgg ctgatattgg
    58141 catgtaacac ttttacccga ctggcggaaa actcattggc aaggattgcc ccttcgttat
    58201 tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc ggcagcgaca tccatcaccc
    58261 gctgtggtgc attaccgtca gcaaacaagg cggcaacagg caacattgaa ctggcttcct
    58321 gaatataaaa caggccactt aaatgctcgg cggtactacc caatggcaat gcatcttcat
    58381 tgtcgcgttc aatccagaaa ccttcttcac accacggaat tggcgtaagc gtccagccat
    58441 aaggagcggt taattgcagg aaatcagcaa cggagatttt cagcgtatta acgcgaatgc
    58501 tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc aaatgagagc gtcgaaggca
    58561 tcgcttcgcg catttgtgtc agaaaggcgt ccgggaaata aacggtgtgt tgggccacga
    58621 gcatgtacca ctggcaaaaa aacaggcgcg cagtttagca taaacgctcc ggcgcaggca
    58681 cgccggagca gtgggcatta tttgggaagc gcagttcccc attcacgcca ctctttcggt
    58741 tcactttcct gcaacaggaa gtgtttgcct tcctgggctt tcggtgccag tggcgtaccc
    58801 ggaggcgtgg cgaaggcgat gccgccacgg ataaactggt taaaggtgcc ggttttcact
    58861 acgccgcccg tcagaccaaa gtccagactg taacccgatg ccaaccagaa gacggaattg
    58921 ttacgcacca ggtgttgata gcgtttactg atgcgcatcg caatcatcac gcgatctgac
    58981 aatgtcccca gcgtcattcc tgtaaccgta ccgacttcca gaccacggaa cagcacaggc
    59041 gtaccgatgc ctaacgaacc ggcttccggc gcttcaacaa taatgcttaa gccatccagg
    59101 taacgcgaat cagtaatggt ggcctcttgt aattcaaagt cgcggcgagg attgccccgg
    59161 cctggttcga cgttgatata cggctggagg atagtatcaa gatgctcaac gccagctgcc
    59221 gaaatttgcg gtgtgaccac tgagaagcgc gtaccaccgc gagcaaaggt ctggacatat
    59281 tccgggtaaa gtaccgcctt tgcctgtact tcattgcgtg cggtaatcag atccagcgtc
    59341 tggatttgcc cgatatcaat accgagatag cgaattggca tcccgaccgc cagttttccg
    59401 gcatcgaaag cgtgaagcgt aatctgcccg ccaaccgcac gggccgctgt ttcggaagca
    59461 tacagaatac gcttatcgcc tttacgctga ctggcgctgg caccgctgag gttgtcgaag
    59521 ctaatggctc cctttaatgc tctggagagc ggggatgcct gtacggtcag accactacca
    59581 ttcagctgaa ctttcgcccc gccttctgcc cagaacacgc tattgctggt cagaaggttg
    59641 cgatactccg gcttaatatg cagatcgata tcaaacgcgt tagctcgcgg acgcacggta
    59701 atcacttcac caacttcaaa tttacggtac agcactaccg atcctgcctg cacatccggc
    59761 agcgtctctg cactcaaact cacggttgtg gtgggtaaat cgctaaggct gttctccagc
    59821 gctttttcca aattggcata cagtggatag ctggctttca tctcaccttt atcgcccggc
    59881 agaatacgta tcccgccatt gatccattct gaggcgctgg caccgagaaa ctcaacgcca
    59941 tccagcccca ccttcacatc gacacggctg ttgacgacaa atttgctatc gccttttacc
    60001 agttcgcgat gctgaggctc gatggcgacg gtaaaggtga cgcctttgct ggtgagttta
    60061 cgatcgataa cctggcctac ctgcacgccg tgaagaatga gcggctgacc cgcatcaata
    60121 ccgtaacttt ccggcgcggt cagggtcagc gtcagaacat caggttcatg cagcagtgct
    60181 ttttcgcctg gcacaacaac gaactctttg cgtggctcgc catcgccggg caccagctcg
    60241 aaggttttgc cggtcagcag ggcgctgaga ttagcatcgc taagggataa tttcgggttg
    60301 cgtaattcga tgcgggtatt ttcacgaagc agggtaacga cgctgggatc aacggtcatt
    60361 tcccctgtga ctttaccacc aggatttaaa tccagtttag tcagctgtcc gacttccagc
    60421 ccctgataca ttaacggcgt cgagtcggcg gttaatccgg ccccactcgg cagttccagt
    60481 tttattatta cgccacgctg gctgtgggcc agatcttcat acagaccaaa ggtatcttcc
    60541 gcctcggcag gtttcgactc ttctggtgaa tcgaaggcaa tcgcaccgtt aaccagtgcc
    60601 gccaggcttt ccagtttcac cttcgcgcca ctgatactga cgttggcatc aacgccggaa
    60661 acgttccaga aacggctacc ttttttcacc agatcggtaa aacgccgctc gatcaggaca
    60721 tcaatcacca cgccttgctt gttgggattg atggcatagt cgtagacttt tcccaccggg
    60781 atcttgcgga aatagaccaa tgaaccgctg ttcagcgaac cgagatcggg ggcttgcagg
    60841 tggatcatca gatcgccatt gtccagccga tatttcggtt gggtatcgag tgcgacaaag
    60901 tgatcctgct ctttaccttt acccggcatc atgccgatat agttcccacc gacgagggcg
    60961 tccagcccgg agacacctgc caacgatgct tttggcgtca ccagccagaa ctgagtctct
    61021 tcgcgcagcg catctttcat atcggacttg atgctgacct tgacttcaat cttacgaaga
    61081 tcgtcgctga ggctgatatc ctgcactgtt ccgacttcaa cgccctgata acgaacaggc
    61141 gtacggcctg gaacaatacc atccgccgac ataaagtcga tggtgacggt attaccccgg
    61201 tcctgataac tgtcccaaat cagccaactg gcaatcatta gcgcgatgaa cggcagcagc
    61261 cagaaaggtg agatacggcg tttattttta atctgcgctt cagtcgtcga agcgggcgtt
    61321 tcctgactca tgtgcatccc aaagtaagcg gctgtccagc cattccacag caagaatagt
    61381 caaaattacc gctgcgccga aataaaacgc agccggtccc atagtaaaag cgaggatctg
    61441 atcgcgatta atcagcgaca tggttaaaga tatgacaaac aggtcgagca tcgaccagcg
    61501 accaatccag gtcaccatcc gcagtaacag aatgcgtgtg cgtaaacctt gctggcattt
    61561 aaaatgaatg ctcaacagta aagtgaacat gacgatcact ttagtaaacg gtaccagaat
    61621 actggcgata aacacgattc ctgcaaccgc aatgttgcta cttgccagcg acataattcc
    61681 ggaaagaatt gtatcttcct gccgtcctcc gttcagataa atgatagaaa taggcaacag
    61741 gttggcaggt aacaataaaa cgattgacgc taacagcgcc gcccagcatt tttgcagact
    61801 atgacggcga cgcaggcgta gcgggatatg gcaacgcggg cagcgaccac gctgatctgg
    61861 atagccggta aaatggcacc caagacagac acgaagtttc tcgtccctac gcgtagcggg
    61921 gcgctgcgga taaaatcgct cccacagttc ttcgacatta agatgtgaca acgtcaccgt
    61981 cgttaaaatc accaacgcca caaaagagaa caagccgaca ccagcctgga tatgggcata
    62041 atcctgtacc tttatagaag caacgccaat gccgaccagg tagatatcga gcattaccca
    62101 ctctttaagt cgctcaagca tcagcagtac cggacgtaaa ttcattccca gtcggttacc
    62161 aaaccataaa taagctatgg aggtcaccag aatgagcggg gcaccaataa cgcagaaaaa
    62221 gaccatcgac cccgttatcg catcgccctg tttggtcatt tgccagatgc cttgcataac
    62281 gttggcgtca atacgaatgc ctaacagcca gatatgcaac agcggttcgc cccaggcaaa
    62341 cggcataagc aacagcatgg tgaaggccat tgccgccagg cgcgttagcg accagtcgcg
    62401 cccgtcacga atttttgcct gacagcgcgg acaataggca ctttgatgag aatttatctc
    62461 gggcaggcta aacagcatgt cacattgcgg gcaacgttgg taatcaccac gcggcagttc
    62521 ctcgccgatt gccctcactg ttatcttttt tgtcggcgta atttgtggtg tgttaagagc
    62581 catgacgtgc caaatgtaat aagaattaac attaatctta acccatgatg ggaatgatct
    62641 tgagcattaa cgcatttaat gcttatttta ataggctggc agatccaggt aagacaacca
    62701 agtgattata taatgaacaa aacagaattt tacgcggatt taaatcgcga ctttaacgcg
    62761 ctgatggcgg gagaaaccag ttttctggca acgcttgcga acaccagtgc gttgttatat
    62821 gagcgtctca ctgacataaa ctgggcaggt ttttatttgc ttgaggacga tacactggta
    62881 ctcggaccat ttcagggcaa aattgcctgt gtccggatac ccgtcgggcg cggcgtgtgc
    62941 ggcactgcgg ttgcccgcaa tcaagtgcag cgtatcgagg atgttcatgt gtttgacggg
    63001 catattgcct gtgatgcggc gagtaattct gaaattgttc tgccgctggt ggtgaaaaat
    63061 cagattattg gtgttctcga catcgatagt accgtcttcg gtcgctttac agacgaggac
    63121 gagcaaggct tacgtcagct tgtggcacag cttgaaaaag tgcttgcaac gacggattac
    63181 aaaaaattct ttgcgagcgt cgcaggataa tcaacggata acgtagcatt tactgatagc
    63241 gtcattataa tgacgcctgt tcatgcctgc gcttgttggc tacgtccgtt gtaatcagga
    63301 aatttcatgg aaaatcaacc taagttgaat agcagtaaag aagtaatcgc gtttctggcc
    63361 gaacgttttc cccactgttt cagtgcggaa ggtgaagcgc gtccgctgaa aatcggtatt
    63421 tttcaggatt tggtcgatcg tgttgctggg gaaatgaacc tgagcaaaac gcaattgcga
    63481 tccgctttac gtctctacac ttcgagctgg cgttatcttt acggtgttaa acccggcgca
    63541 acgcgtgtcg atcttgacgg caacccatgc ggtgagctgg acgagcaaca tgtagagcat
    63601 gctcgcaagc agcttgaaga agcgaaagcg cgtgttcagg cacagcgtgc tgaacagcaa
    63661 gcgaaaaaac gcgaagctgc cgcaactgct ggtgagaaag aagacgcacc gcgccgcgaa
    63721 cgcaagccac gtccgactac gccacgccgc aaagaaggcg ctgaacgtaa acctcgtgcg
    63781 caaaagccgg tagagaaagc gccaaaaaca gtaaaagcac ctcgcgaaga acagcacacc
    63841 ccggtttctg acatttcagc tctgactgtc ggacaagccc tgaaggtgaa agcgggtcaa
    63901 aacgcgatgg atgccactgt attagaaatc accaaagacg gcgtccgcgt ccagctgaat
    63961 tcgggtatgt ctttgattgt gcgcgcagaa cacctggtgt tctgaaacgg aggccgggcc
    64021 aggcatgaac atgtttttta ggcttaccgc gttagctggc ctgcttgcaa tagcaggcca
    64081 gaccttcgct gtagaagata tcacgcgtgc tgatcaaatt ccggtattaa aggaagagac
    64141 gcagcatgcg acggtaagtg agcgcgtaac gtcgcgcttc acccgttctc attatcgcca
    64201 gttcgacctc gatcaggcat tttcggccaa aatctttgac cgctacctga atctgctcga
    64261 ttacagccac aacgtgctgc tggcaagcga tgttgaacag ttcgcgaaaa agaaaaccga
    64321 gttaggcgat gaactgcgtt caggcaaact cgacgttttc tacgatctct acaatctggc
    64381 gcaaaagcgc cgttttgagc gttaccagta cgctttgtcg gtactggaaa agccgatgga
    64441 tttcaccggc aacgacactt ataaccttga ccgcagcaaa gcgccctggc cgaaaaacga
    64501 ggctgagttg aacgcgctgt gggacagtaa agtcaaattc gacgagttaa gcctgaagct
    64561 gacaggaaaa acggataaag aaattcgtga aaccctgact cgccgctaca aatttgccat
    64621 tcgtcgtctg gcgcaaacca acagcgaaga tgttttctcg ctggcaatga cggcgtttgc
    64681 gcgtgaaatc gacccgcata ccaactatct ttccccgcgt aataccgaac agttcaacac
    64741 tgaaatgagt ttgtcgctgg aaggtattgg cgcagtgctg caaatggatg atgactacac
    64801 cgttatcaat tcgatggtgg caggtggtcc ggcagcgaag agtaaagcta tcagcgttgg
    64861 tgacaaaatt gtcggtgttg gtcaaacagg caagccgatg gttgacgtga ttggctggcg
    64921 tcttgatgat gtggttgcct taattaaagg gccgaagggc agtaaagttc gtctggaaat
    64981 tttacctgct ggtaaaggga ccaagacccg tactgtaacg ttgacccgtg aacgtattcg
    65041 tctcgaagac cgcgcggtta aaatgtcggt gaagaccgtc ggtaaagaga aagtcggcgt
    65101 gctggatatt ccgggcttct atgtgggttt gacagacgat gtcaaagtgc aactgcagaa
    65161 actggaaaaa cagaatgtca gcagcgtcat catcgacctg cgtagcaatg gcggtggggc
    65221 gttaactgaa gccgtatcgc tctccggtct gtttattcct gcgggtccca ttgttcaggt
    65281 ccgcgataac aacggcaagg ttcgtgaaga tagcgatacc gacggacagg ttttctataa
    65341 aggcccgctg gtggtgctgg ttgaccgctt cagtgcttcg gcttcagaaa tctttgccgc
    65401 ggcaatgcag gattacggtc gtgcgctggt tgtgggtgaa ccgacgtttg gtaaaggcac
    65461 cgttcagcaa taccgttcat tgaaccgtat ttacgatcag atgttacgtc ctgaatggcc
    65521 agcgctgggt tctgtgcagt acacgatcca gaaattctat cgcgttaacg gcggcagtac
    65581 gcaacgtaaa ggcgtaacgc cagacatcat catgccgacg ggtaatgaag aaacggaaac
    65641 gggtgagaaa ttcgaagata acgcgctgcc gtgggatagc attgatgccg cgacttatgt
    65701 gaaatcagga gatttaacgg cctttgaacc ggagctgctg aaggaacata atgcgcgtat
    65761 cgcgaaagat cctgagttcc agaacatcat gaaggatatc gcgcgcttca acgctatgaa
    65821 ggacaagcgc aatatcgttt ctctgaatta cgctgtgcgt gagaaagaga ataatgaaga
    65881 tgatgcgacg cgtctggcgc gtttgaacga acgctttaaa cgcgaaggta aaccggagtt
    65941 gaagaaactg gatgatctac cgaaagatta ccaggagccg gatccttatc tggatgagac
    66001 ggtgaatatc gcactcgatc tggcgaagct tgaaaaagcc agacccgcgg aacaacccgc
    66061 tcccgtcaag taatatcaat caggcacaag aaattgtgcc tgatttttta acagcgacaa
    66121 gatgccgtaa atcagatgct acaaaatgta aagttgtgtc tttctggtga cttacgcact
    66181 atccagactt gaaaatagtc gcgtaaccca tacgatgtgg gtatcgcata ttgcgttttg
    66241 ttaaactgag gtaaaaagaa aattatgatg cgaatcgcgc tcttcctgct aacgaacctg
    66301 gccgtaatgg tcgttttcgg gctggtactg agcctgacag ggatacagtc gagcagcgtt
    66361 caggggctga tgatcatggc cttgctgttc ggttttggtg gttccttcgt ttcgcttctg
    66421 atgtccaaat ggatggcatt acgatctgtt ggcggggaag tgatcgagca accgcgtaac
    66481 gaaagggaac gttggctggt caatactgta gcaacccagg ctcgtcaggc ggggatcgct
    66541 atgccgcaag tggctatcta ccatgcgccg gacatcaacg cttttgcaac cggtgcgcgc
    66601 cgtgatgcct ctctggttgc tgtcagcacc ggtttgctgc agaacatgag cccggatgaa
    66661 gccgaggcgg taattgctca cgaaatcagc cacatcgcca atggtgatat ggtcaccatg
    66721 acgctgattc agggcgtggt gaacaccttc gttatcttta tttcccgtat tctggcgcag
    66781 cttgccgcgg gttttatggg cggaaatcgt gatgaaggtg aagagagcaa cggcaacccg
    66841 ctgatctact ttgcggttgc aacggttctg gaactggtgt ttggtattct ggcgagcatt
    66901 atcaccatgt ggttctcgcg tcatcgtgaa ttccatgctg atgccggttc ggcaaaactg
    66961 gttggtcgcg agaaaatgat tgccgcgctg cagcgcctga aaaccagcta tgaaccgcaa
    67021 gaagcaacca gcatgatggc tctctgcatt aacggtaagt cgaaatcgct cagtgagttg
    67081 ttcatgaccc acccgccgct ggataaacga attgaagctc tgcgtacggg tgaatacctg
    67141 aagtaatatg aagtaaatac taaaaagcgc gtcctgatcg acgcgctttt tttatgccct
    67201 ggatcgtggc tgagtgatac gtaaaccact gacacaggca gcaatcactg ccagaatagc
    67261 cgcagccatc agcgagacgt gtgtaccatt atctccaaac tgatttagca tcagcgccac
    67321 cagcgccgcg ccgctactct gacccagtag acgagccgtt cctaacatgc cactggctcc
    67381 accgctacgt tcgcgaggcg cggaggtaat aatggtgtgg ttattgggtg actggaataa
    67441 gccaaatcca gcaccacata agatcatcgg ccagataata ttgatatccg caggtgacgc
    67501 gggcagcaga accagggaaa aaagccccgc agccatgatg aacaacccta aagcccccag
    67561 caatcctgca tgtacgcgtt caatcaaata gcctgccagc ggagccatca ccatcgttgc
    67621 taacggccac ggtgtcagaa gtaaacctgt ttcgacttca ctacgcccga gcacggtttg
    67681 caggtaaaag ggcagggaaa ccattgccag catttgtgcg cagaaagagc aaacagatgt
    67741 gcaaatagaa agtgaaaaca gcgggatacg cagtaaatcc accggtagca gcggtacggg
    67801 aagagaaagc tggcggcgaa taaagaaaat accaacaaca accattacca ccagttccgc
    67861 agcaattaac gtcagcgatt gcccctgagc gaaaccactc aacgcagtga taagcaggcc
    67921 gaaggttaac gcgttcatca cggcgctggg caggtcgaaa cggggtttac tggcgcgaga
    67981 accattgggt ggcagaaaac gcatcgccag aagcagggcg ataataccta acggtacgtt
    68041 gattaaaaat aaccatttcc aggatgcgat ggagaggatt gctgcagcaa ttgtcggccc
    68101 ggcagcagaa gagacggcaa caataaacga gtttatgccc atccctctac ccagaaaacg
    68161 ttgtggatag atcaggcgga taagtgcggt attaacgctc atcaacgctg caccgccgaa
    68221 accttgtatg acacgcgcaa gggtgagcat ttgcagcgaa tcagaaaggg cgcagaacag
    68281 tgaagacaac agaaaaacga ccagaccgca tttataaata cgtcgatagc caaacatatc
    68341 gcccagaaac gaaaacgaga gcagggagat gacaatggcg atttgatagg cgttcactac
    68401 ccagatggaa ctggctggcg tggcatgaag gtccgtggcg attgttggca gggcgacgtt
    68461 ggcgattgcg ccgtcaagga cggccatcga aataccaatc acaatggtta atatcgcacc
    68521 gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt ggcataggaa ttttatatct
    68581 ttggtgaata atcaacaggg gtatgattct agcactgata ttcacggtgg atgtcgcaga
    68641 attgtaacta accggcaaaa gatggattgc gaccaccctg gtgcgaaatt ataataaaaa
    68701 acagttctga tttttataaa acactcgcaa tgaggtgata aatggctaac gcagatctgg
    68761 ataaacagcc tgattctgta tcttccgtgc taaaagtttt tggcattttg caggcgctgg
    68821 gtgaagagcg cgaaataggg ataaccgagc tgtcgcagcg cgtcatgatg tcaaaaagca
    68881 ccgtttatcg ctttttacag accatgaaaa ccttaggtta tgtggcgcag gaaggggagt
    68941 cggagaaata ttccctgacc ctgaaattgt ttgaactggg cgctcgcgcg ttacaaaacg
    69001 tcgatttaat tcgtagcgca gatatccaga tgcgtgagct ctcccgcctg accaaagaaa
    69061 ctatccacct cggcgcactg gacgaagaca gtattgttta cattcacaaa attgactcta
    69121 tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa tccgctgtac agcaccgcga
    69181 ttggtaaggt actgctggca tggcgcgatc gcgatgaagt gaagcaaatt cttgagggcg
    69241 tggagtataa acgcagtacc gagcggacca tcaccagtac agaagcgtta ttacccgttc
    69301 tggaccaggt gcgcgagcag gggtatggcg aagataatga agagcaggaa gaagggctgc
    69361 gatgcattgc ggtaccggta tttgatcgct ttggcgtggt cattgccggt ttgagcatct
    69421 ccttcccgac gttgcgtttc tctgaagagc gtttacagga atatgtcgca atgttgcata
    69481 ccgcagcgcg caaaatttct gcccaaatgg gttatcacga ctatccgttc tgatgagagt
    69541 aagaacctgt cggaatatca aacagacagg ttctttattt agcatgagaa aaataaagtt
    69601 gaaggtggcg ttatattaaa cgcgcttgct ataagagtat tttactcagg agtgagaatc
    69661 tggttattta ttgcccttaa ccattatcga ccacgatatt gcttttgcgt aacagcgggc
    69721 aatctgttat ccccaaaaaa ccacttttag tgtgcaagta ttgtaccgtg ctggtgcctc
    69781 tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg cttccaatca
    69841 aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc ataactgtgc
    69901 gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg tagcggcaat
    69961 atgcgctgaa gcaagcgact cattccgaaa aagcacgaat atcgacatag ttaggcgctg
    70021 tttaactaac gcatgctagt ttaatgacat aaggtaggtg aaacggagat tggagtgaaa
    70081 aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct ttgggcgcag
    70141 gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg tgccattaac
    70201 cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc tccagcagtg
    70261 gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat tcaggcgtga
    70321 tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc atcttgtggt
    70381 tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt gaggcgttgc
    70441 tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca aacgaaccag
    70501 agaaagcaga taaaaagacc gttgagagtc aaaaatcggt tgaagacgaa gatattattc
    70561 gtctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt tgaagggtta
    70621 ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt aaaactctgg
    70681 tctgacttca aataattatc atattattta tttgcgtaac gggatatcag aaaaagatat
    70741 ttaccccatt aatttattag gatgtttaca tcggatttgt gattaagcgt ggtattattt
    70801 attagcgaaa cgtttctctc ttgatttttt tgctcattca tcaatttttc ttattttaaa
    70861 tttacaatcc ctttggggat tgacttctct ttagggtaat taatagccgt taactgactg
    70921 ttttatgaga aaaagtgata taacttttta ttcattgcat agcaaaaaat gtgatattgc
    70981 acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt gaactatgtt
    71041 agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca atgatatatc
    71101 ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg
    71161 tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt gataacagat
    71221 ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa gaagtcctga
    71281 gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt gcctcatgca
    71341 gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt
    71401 ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa ggttttggct
    71461 tcattactcc ggctgatggc agcaaagatg tgttcgtaca cttctccgct atccagggta
    71521 atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag gacggccaga
    71581 aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc tgaagtgtga
    71641 atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact atgctttatc
    71701 ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact tgcggccttt
    71761 agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag cctctttcgc
    71821 gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg aaagaagggt
    71881 atgtcaatct gctgcccgtt cagcataaac ggtctcgtga tccgggcgac agcgcggaaa
    71941 tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg cgtgatgcaa
    72001 ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg gatattggct
    72061 gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg
    72121 gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg caggtcactt
    72181 tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac gccataatac
    72241 gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc ggcggctggg
    72301 tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg atttacaatg
    72361 aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta cagcagagtg
    72421 cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga
    72481 cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac actggcagca aaagaagtgt
    72541 tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa ccgtggaagt
    72601 gcgtccagag gatctggacg ccgatgccga tcagcaccag cccgccgaga atttccgctt
    72661 ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt gacataatca
    72721 aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg aaagcaagac
    72781 caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc agtagccaga
    72841 aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa ccctcaataa
    72901 tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc cattcaagga
    72961 caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg gtttcgacgg
    73021 caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg agggtggcac
    73081 ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga agaacagtag
    73141 cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca tcgcccccag
    73201 caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca ggcatagcga
    73261 ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc cacgttttgc
    73321 aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc ccaaatgagg
    73381 gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt atttcattgc
    73441 tttattgata acgattttca tttggattta tatatcaatg aaacgttaaa ataaataaca
    73501 aataagggaa gaggtaatat agcctaagct atgtctgatt attaaaaata cactaaacat
    73561 agctttggct aaattcataa cttattgagt tgaaacgaga agcttataaa ttttttccag
    73621 atcgtcgata tttcgaacgc ggattaacag ccgacgctgt tctaattgca tcaccaacac
    73681 gccatcttcc gacaagttca tcgctttgat tcggctatat tcaatccaga cattggcgaa
    73741 gaaaaaacct ttttgtttaa agatgatctt cggaacgcgg atccagaata tataaaaacc
    73801 catcagagcc agtgcgctta ataaccatgt cgttattaac gcaccatgat tcgtgacgtt
    73861 gttatagata agaatgacaa tcaatccgac gaagataacg ctatcgatgc gaccacgccg
    73921 gagcaaagga attgccagca gggtggggcc gttacggcgg ggcatgatga actgatcgta
    73981 gatcgcgaag gccaggagtg cggcgatgaa aagaatcagt accaggtccg tgattgtcat
    74041 taatcctcca gataaaaaaa cggggccaaa aggccccggt agtgtacaac agtcttacag
    74101 tcccagcagg ccgcaagcgt aaccagcgat accgatgacg aagaagccaa cgatgatcca
    74161 cagcgggtta acttttttgc gcagtagcca catacaagca aaggtcagca gcagtggtac
    74221 caggcctggc attaactggt ccagaatagt ctggacagta gtaacgtgtt ctttgcccgt
    74281 ctggtcagta atgcgagaga caaccagcgg gatgttgaca tgtgtccact tgttaaccaa
    74341 tgcccccatg acaaacaggc cgaggataga cgccccttcc gtcagttttt gcaggaagcc
    74401 accacccata tctttaacga tatcgatacc tttggagtaa ccatacgcta cgccgtagta
    74461 acgggttgcc agacgcacca ggttaaacag gatgaagaac agcagcggac ctaacaggct
    74521 gccgctcatc gcgataccgg cacccagtgc tgcaaatacc ggacgtacgg ttccccagaa
    74581 gatcgggtcg cctacaccag ccagtggccc catcaaaccg actttgatac cgttgatagc
    74641 accgtcgtcg atctctgcgc cattagcacg ctgttcttcc agcgccaggg ttacgccgag
    74701 aatcggcgca gccacgaacg gctgggtgtt aaagaactcc aggtgacggc gaatagcttg
    74761 tttacgagct tcgttgttct cagggtagag gcgacgaatt gccggtacca tagagaagca
    74821 gaaacccagt gcctgcatac gttcgaagtt ccatgaaccc tggaagaggt tagaacgcag
    74881 gaagacgcca cgaatatcac tttgagtgag ttttttctcg gtggtagttt gagttgtatc
    74941 aaccatttcg ctcacctgtt agtccagttc gttatcgaga tcgttgttac cagctgcctg
    75001 agcaggcgca ccggctacgc ggttgtattt cgggctaagt tggatgtaga gcactgccat
    75061 aacagtacca atcacaccca gagcaaccag gttaaagttg gtgaatgctg cggttacgaa
    75121 gccgaggtag aagaacggca tcaggtagcc agcacgcatc atgttgataa ccatcgcata
    75181 accaaccacc acgatcatgc caccggcgat attcagacca ttggtcacca cttccggaat
    75241 cgcattcagc atgttctgta cttcgctggt accaacagac agcgcaacga tgacggccgg
    75301 aatagccaca cgcattgctt gcaggaacag agaagaaacg tggatccagg aaatcgctgt
    75361 caggttgccg ttatcagcag ccttatccgc agcgtgctgg aaagcaacgg taatagtacg
    75421 aacgatgatg gtcagtacct ggcccgcagc ggccagaggg attgccagtg cgatacctgc
    75481 accaatgctc tgatgacctg cgataaccag aatggtagaa atgatagaag ccagagcggc
    75541 gtcaggcgca actgcagcac cgatgttcat ccagcccagc gcgatcattt ccagcgtacc
    75601 accgataata ataccggttt tcatatcccc aagaacgata cccaccaggg tacacgcgat
    75661 tagcggacgg tgaaactgaa attcatcgag gattgatccc atacctgcga tacaggctac
    75721 gataaatacc agcacaattt gaagagtggt aatctccatt gtacttctcc tgtttacgta
    75781 agacttaagt gtgaaaactg agtgataatc aacacaatac gttacttatc gattttgctg
    75841 atcagatcca tcattttcag tttcggatcg gtggaaacct tacggacttc cagctcaata
    75901 ccgcgcgcat tcagtttctt gaacgcctcg atatcttttt catcaaccga aaccgcgtta
    75961 ttcacctggg ttttaccctg acggaatgcc ataccaccga cgttaacaga ggtgattttc
    76021 acgccgcctt caacgagacg ctctacatct gttgggttgg taaataacag cattacgcgt
    76081 tcgccagcat atttcgggtt gttgtagacg cgaatcattt tggcaacatc aactacgtgt
    76141 gctgttacgc ccggaggtgc aacctgggtg agcagtgtct tacgaacggt atccgcagcc
    76201 acttcatcac taacaacaat aatacgggag acattggttt ctttggtcca gcgggtggcg
    76261 acctgaccgt gaatcagacg gtcgtcgata cgcgcaaggc caataaccat gtagtcgttt
    76321 ggccccattg gttttgccgg agttggagcc gcttttggtg ctgcggcagc gggtgctggc
    76381 gcggcttttt caaccggttt ggctttcagt gctttcacgc cttcacggcc tgtttctact
    76441 gccagtgcca ccagttcatc aaagcttggg tcatcatcac gggccattaa cgtttccacg
    76501 agcattggaa tgttaacgcc tgcaatgact tcataatgct ctttgtcgac gacaatgcgg
    76561 ctggcagcat tgaacgggct gcctccccat gtatcaacga gaaacagcac gcctttagtg
    76621 gtgtcgagtt ttgccaactg agcgttgtac ttttcaatca gcgtttcggc attttcacct
    76681 ggaacgaaat cgatccagcc gacgttttcc tgctcgccta acagcatttc tgccgtttta
    76741 agcaactgct ctgcagccca accatgtgtg cctataacaa tagcaatggt cacttgctac
    76801 ctcctttatt atcgttaaca cctcaacgtg ccagatgtat ttttgaatcg tcgtccacaa
    76861 tcgaatcgat tcagataagg gctacaacca aaaacccctg cgtttcgcga tttattttag
    76921 atatcgaaaa aattatttta tgtgatgaag atccgtaatt taactttcga ttagcagaaa
    76981 tttcgaaagg taaaatatcc ttgtcacatt cgtttgcaaa ggaaggtaaa tctttgccaa
    77041 atcagaggcg tctctgatat gtttaactcc cgtttaacaa ccatggagta tagggcagta
    77101 gcccgcagta tggatcgtca ccgacgtcat ttcagcatca ggccttttaa cgcctgcctt
    77161 tctggcactc tatgccgcac ctttcgtttg cattttgtcg ttacgcctgc attatttctg
    77221 gcgtcgaata gctattcctt aagcaggagc ttgtcatgga attcttaatg gacccctcaa
    77281 tttgggcggg gctactcacg cttgttgttc tcgaaattgt gctgggtatc gataacctgg
    77341 tcttcatcgc cattcttgct gacaaactgc cgccaaaaca acgcgataaa gcgcgtttgc
    77401 tggggttatc actggcgctg attatgcgtc tggggctgct gtcgctgatt tcatggatgg
    77461 tcacgctgac caaaccgcta tttaccgtca tggatttctc cttctccgga cgcgacctga
    77521 ttatgttgtt cggggggata ttcttgctgt tcaaagcaac aaccgaactg catgaacggc
    77581 tggaaaaccg cgatcatgat tccggccacg gtaaaggcta cgccagtttc tgggtggtcg
    77641 tcacacagat tgtcatcctt gacgccgtct tctcgttgga tgcggtaatt actgcagtag
    77701 ggatggttaa ccatctgccg gtgatgatgg cggcggtagt gattgcgatg gcggttatgt
    77761 tgctggcatc caaaccgctg acgcgattcg ttaaccagca ccccacggtg gtggtgctct
    77821 gtctgagctt cctgttaatg attggtctga gtctggtggc agaaggtttc ggtttccaca
    77881 ttccgaaagg ttacctgtat gccgcgattg gcttctcgat catcatcgaa gtgtttaacc
    77941 agattgcgcg tcgcaacttt attcgccacc agtcgacttt gccgctgcga gcgcgtactg
    78001 ccgatgccat cctgcgtttg atgggcggga aacgtcaggc caatgttcag cacgatgccg
    78061 ataacccgat gccgatgccg atcccggaag gtgcatttgc cgaagaagaa cgttacatga
    78121 ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg gatcatgacg ccgcgcggtg
    78181 aaataagctg ggttgacgct aatctcgggg tcgatgaaat ccgcgagcaa ctgctctctt
    78241 caccgcacag tctgttcccg gtatgtcgcg gtgaactgga tgaaatcatc ggtattgtac
    78301 gtgctaaaga actgctggtg gcgctggaag agggcgttga tgtggcggcg attgcttcgg
    78361 cgtctccggc gattatcgtc ccggaaaccc tcgatccgat caacctgttg ggcgtgctgc
    78421 gtcgtgctcg cgggagcttt gttatcgtga ccaacgagtt tggtgtggta caaggtctgg
    78481 tcacgccgct ggatgtgctg gaagccattg cgggtgaatt cccggacgct gacgaaacgc
    78541 cggaaatcat tactgatggt gacggctggc tggtaaaagg cggtacagat ttgcatgcct
    78601 tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga tgatatcgcg acggtcgcgg
    78661 gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg cgatgtgatt gatgtagggc
    78721 cactgcatat caccatcatt gaagccaatg attatcgtgt tgatctggtt cgcattgtta
    78781 aagagcaacc ggcgcacgat gaagatgagt aagcgttaac gtaacggcat aatgggcgtg
    78841 atatgtccat tatgccgggc gggcggcggt tggctgcccg ccagccattt gggaaaatca
    78901 cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa tatgcacgcc atgacggcgc
    78961 aaatacttcg cctgttcttg cgtttccacg ccctccgcca ccagttcaat attcagtctt
    79021 tgccccagcg cgatgatgat atcggtcacc gtcgagttaa ccgcgtcaga acctatagct
    79081 gcggtaaatg acttatcaat tttcagcacg tcaggacgta atgtttcaag ctaggaaaac
    79141 gagctgttgc cggtgccgaa gtcatcaatc gccagtttga cgtttttacg atgcagctcg
    79201 cgggcaatcc gataatcaac atccagtaag gcatcgcgtt cggtgatttc gaggatcagt
    79261 tgctgaatcg ggtgagcgct aaaccagtac tgattgagat cttttatcag cacaccacgg
    79321 cgaaaatggc tgggtgcgac gttaatgcca acatgaaact gactactcat cgggaaaaca
    79381 tggcgctgac gaatggtttc tgccatcaca tagcgggtca gtggcacaat taaatgatgt
    79441 tcttccgcga taggaataaa cacatccggt gaaatccagc cctgacgcgg attgttccag
    79501 cgcagcaaaa tctctacacc aatacattgc tggctgcgcg cattaagcaa aggctgacag
    79561 aacaattcga actcatgttg cgccaggccc agattgattt cgcgggaaaa gctcattcgg
    79621 taagcggtcg ccagccaggc gatgtagccc accagtaaac ttagcagcac cgccagcggt
    79681 aattgtgttg gaagatagtg ccatgccagc gccgtagccc ccggaccatt aacgttaatg
    79741 gtaaaaggaa agcgttgcga agaaacctga tagttttcat tgtcttcagg ttgcggaagg
    79801 gaatctacca gcccattacc atagagcaaa tgccgtttgt ccaccgtcag gctggcgcta
    79861 ctgatttgcg gcagttgtgg ctcaagcagc attgccgtca gtaagtcgat gttaatcatc
    79921 tccatgaccc cagcattgct actgcccgct gcaggcgtcc attgaatcaa aaccggactg
    79981 cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg gttgcggtgc aggaagttca
    80041 gccagaatgt ccacgacggg cacattgcga taaccaaaaa tgctggagca ataaagtgtg
    80101 ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt ttgccgcctg tttacgtaat
    80161 ggcaaatggg cgacagagca gggcagacca atcagcggaa gcaagacttc gctacctgcc
    80221 tgtaggggaa gcagtacttt atctaattcc tctacagcgt gattggcgaa ttgtactacc
    80281 cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc tcagggtcga agcgagcgta
    80341 acgagtgcgc aaatcgtaca aacaatcatt cgattacgac gataggtttt aatgatccgt
    80401 tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg acaaacgcct gacgcaacag
    80461 tggaagtgta gtggggaata ttacagcaga cgagaaagcg ggtataaatt cgcccatccg
    80521 ttgcagatgg gcgagtaaga agtattagtc acactggact ttgattgcca gaccaccgcg
    80581 tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt tcgtacatcg tttcgatgac
    80641 cttatccagc gagacgcgcg gtgcactggt gcggcgcaga gccatccgcg cggcgttaat
    80701 cgccttcaca gaggcaatgg cattacgctc aatgcacggc acctgaacct gccctgcaac
    80761 cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt tccgccgcca cgcaaacctg
    80821 ttccgggcta ccgcccagca gttctgcaag acccgcagca gccattgaac aggcaacacc
    80881 cacttcgccc tggcaaccaa cttccgcacc ggaaatagag gcgttcattt tatacaatgc
    80941 accaatcgcg cccgctgcca taaagtaacg ggtatagatg tccgggctga ccgattcaat
    81001 aaagtggtca tagtaagcca gcactgccgg aacgataccg caggcaccgt tggttggcgc
    81061 agttaccaca cgaccaccgg cggcgttttc ttcgttaact gccagcgcaa acatgtttac
    81121 ccagtcaatg acattcatcg gatcgttaga cagtttatcg ctggaaacca gcatccggcg
    81181 cagggcagac gcacgacgtg gcacgcgcag cgggcctggc agtacacctt cggtgttcat
    81241 cccgcgatcg atacatgcct gcatggtttg ccagacatgc gcgaaatact cgtcgatctc
    81301 tttcttgctg tgcagcgcca gttcgttctg catagcgaga ccagacagcg aatagccggt
    81361 ttcattacag tacgcgagca gttcggtggc agatttgaac ggatacggca cgcttacttc
    81421 gttggcagca tcctgaccaa agtgttcttc atcgacgata aaaccgccgc cgatggaata
    81481 ataagttttg ctgtagacga cttcatcgcc gttataggcg tggatttgca taccgttttc
    81541 atgcagcggc aggttgccgt tatgaaaacg catcccgttg tcgcgcggga aatccacttc
    81601 atgccgtccc tgtgccagca gcagacgttc gcgctcttct acgtcgcgaa taaaaccggg
    81661 aatactgtcg atatccacgg tggcaggttc gttacctgca agacccataa taatggcgat
    81721 atcggtgtgg tggcctttac ccgtcagcga cagtgaacca taaacgtcca cggcaacgcg
    81781 agtaacgcta tccagtaagc ctttttcgac cagatcatcg acgaactgtt tacctgcctt
    81841 cataggccct acggtatggg aagatgaggg accaatcccc accttaaaca tgtcgaatag
    81901 actaatcacg ataatactcc tgacaaggtg actggacttc cagtaacgaa ctaataacag
    81961 cgcatagtgt aagagggaac gcggcgactg gcttaactat tcacatgaat taaactaatc
    82021 aaaaaccgcg atttaactaa tattgcgatg attgtctcaa gtagtgtttt gtagatgtaa
    82081 agataagtat agtcagggtt tcacaccaat ttgcagcgcc agctcacgaa ttatgcctgc
    82141 ggtcattccc catacaaaat actgttcgta ccaggacagc catacccgat gtgaatcacc
    82201 acggcggtag atatctaaag ggtgataacg acccagatgt aatgcctggg cgagcggcat
    82261 ttcaaacacc gccgagactt catcttcact ggcgcgatac ggcagatcgg gcgggataat
    82321 gccgaccact ggggttacct ggtagccagt gacgctatcg acgggcggca gcacgccgat
    82381 aacttcaacg gcggaaggcg gtatagcgac ctcttcttca gcttcgcgca gcgcggcggc
    82441 gatagctgat gcgtccgtgt catcgactgc acctccaggg aatgccactt gtccagcgtg
    82501 tttacgcaga tgaatcgaac gctgagtcag caacaacccc ggttgcggtc gacggacgat
    82561 ggggattaac acagcagcct gacgatgatt tagggtttcc cggttaattt gcgggcgcaa
    82621 aagttgaaag cgcgataaaa aatcatcaag cgtcaggcta cggtattcca cgtcttactt
    82681 ctccagttgc ttcaggatac gattaacttt atcaaaagtt tcctgatatt ccgcttcttc
    82741 ctggctatcg gcgacaattc caccgcccgc agagcagaaa atttgtccgt taatggcagt
    82801 cagcgtgcgg atagtaatac tggtatccat gttgccgcaa aagctcaaat agccaatgct
    82861 gccgcaccag gaattgcgtc gctgcggttc cagttcgtcg ataatttcca tagcccgtac
    82921 tttcggagcc ccggttattg agccaccagg aaaagctgcg cgcagcagat cgctggcgtg
    82981 taactgttct ggtagttgcg ccgttatggt gctgaccaga tgatgcacgg cagggaaggg
    83041 ttccaccacg aacagctctg gtacttttac cgaacctgct acggcaacac gaccgatatc
    83101 attacgcatt aaatcgacaa tcatcagatt ttcggcacga tctttcgctg agttcgccag
    83161 ttttactgct tgtttgctat cttcctgagg atcgggcagg cgtggtagcg tgcctttaat
    83221 cgggcgggtc tggatttcac tattatcaca aagaataaac cgctctggcg aaaggcttaa
    83281 aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc gcgcggttgg cctgattaag
    83341 ctgaaggaat gcctgccatt catcgccaga ataggtcgca tgaaaacgtt gggcgagatt
    83401 cacctgatag caatcaccgc tgtgcagata ttcctgtacc tggcgaaatt tttcgccgta
    83461 ctgctcgcgg gtcatattgg attgccagtc ggaagtgagc gtgaaatctt cctgcggcga
    83521 gaattgctgg ctttccagcc aggcccgacg ggcattgaca tcattatgac tcagcaaaga
    83581 aactgtatga cgctggtggt cgacaatgag cgcccaatcg tagataccca ctgccatatc
    83641 cggcagaacg atatcttgtt ccgcaatttc tggcagtgac tcaaaacggc ggcccagatc
    83701 gtagccaaac aaccccagtg cgccgccctg aaatggcaaa tcttcgttat gcgttgggcg
    83761 aatgtctgcg cgatccagca cctgctggag cacctgtagc gggtcatcag tggtcgttgt
    83821 gcgtttttcg ctttcactaa caacggtttc tttaccgaaa gtggttaaag tgcaaatcgg
    83881 ctcggcgacc acaatatcaa agcggctata cggatgatcg gcatagccgg agtgtaaaag
    83941 catcgcccac ggcaggtggc ttaagcggga gaaataaaat tcagcggcgt cctgacgcca
    84001 gggtaaagta atcacagcgg gagataacgt cttcatcagt cctgactcta ctggctatgt
    84061 gccgggtagc atgaaataat tagcgccaac aatttagcag gagttaacaa tgtttgcagg
    84121 tttaccttca ctcacccatg aacagcagca aaaagctgtc gagcggatcc aggaactgat
    84181 ggcacaaggg atgagcagcg gccaggcaat cgcgctggtg gcagaagagc tgcgtgccaa
    84241 ccactccggc gagcgaatcg tggcgcgttt tgaggatgaa gacgagtaac acactaatcc
    84301 gtatcgattt cgttatagaa gccgatgatc atttgattgt gagaaaaagg acgcttttta
    84361 tatgtgatta aaagcgtcct gacccattta tgctgatata aatttaactc agaccagtgc
    84421 ttttagttct tcctgcatat atgttgaaaa gtattcagga tttttaatca cagaacgttc
    84481 acctgtctcc attttttttg cccatccaac cattttttcc gtgaaccctg ggttccagcc
    84541 ttcctggtca gctcgcgccg taatatcagc ggcactcgcg ggtactccca gttcttttaa
    84601 atatttaaag attcccttcg ccgtgctttc attcatcgaa cttggtgttg gcgctgatac
    84661 atccatggca ccaatgaaat ccagggcttt atctattaca gcaggcatat tcttatcctt
    84721 aaaattaacc atgttatcga taaggctata tatgacctta atttgcatct aatatcaaag
    84781 ggattataac gatatggcac gagttaacct gttgacattc aggtgtttta aaggtagcag
    84841 ctcatggtgt gttatccagt aagatggaga aatttaacgg tcagtccgca cgctataacc
    84901 agattgttca gctaagaaag aaaaccagag ttgctaatca aagggcgaac acaacggcat
    84961 gtttatgttt atttatatag ttagttttat agataaaatt gaaattataa atataaataa
    85021 tgaattagca gaagcatcaa cagtaatatc ttattctctg gtcgatttat gtaaaaccga
    85081 ccagtattat cgataaatta tcgtctgact tattttgctt cgataaagct tacaccgcag
    85141 ccacaatctt aatttcaact ttatacttcg ggttcatcaa acccgcttgt accgtgcagc
    85201 gcaccggcgc atgacccgcg acaacccagg catcccacgc tttattcatc gccgcgaagt
    85261 cgtttttatc ggccaggaaa atggtggcat ccagaatgct cgatttattg ctgccctgtt
    85321 tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt ttgctcaaag gcatcggcgt
    85381 cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt gtggattact acatcagacc
    85441 agcgggcttc agcatcgata cgaacgatag tcatcatttt gtcctcatta tacttccatt
    85501 ttcgtgctgc aaagactgcc acaatgtgag gtcgccgtca ccacttcaac tggcgaaagc
    85561 gccctcatcc tgacataatc cctgttcaaa tcaacagggg gtagtgtgac ggacgatttt
    85621 gcaccagacg gtcagctggc gaaagcgata ccaggcttta agccgcgaga accacagcga
    85681 cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc agccgctggt ggtggaagca
    85741 ggaaccggta cgggcaaaac ctacgcttac ctggctcctg cgctgcgggc gaaaaagaaa
    85801 gtcattatct cgaccggctc aaaagcgttg caggatcagc tctacagccg cgatttgcca
    85861 acagtctcaa aggcattgaa atatacgggc aacgtggcgc tgctgaaagg gcgctcaaac
    85921 tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg ggggcgatct gccggtacaa
    85981 atcttaagcg atgtgatcct gctgcgctcc tggtctaatc aaacagtcga tggtgatatc
    86041 agcacctgcg tcagcgtggc ggaagattca caggcgtggc cgctggtcac cagcaccaac
    86101 gacaactgtc ttggcagcga ctgcccgatg tataaagatt gttttgtggt caaagcacgt
    86161 aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc atctctttct ggcggatatg
    86221 gtggttaaag agagtggatt tggcgaactg atcccggaag cggacgtcat gatcttcgac
    86281 gaagcccacc agctaccgga cattgccagc cagtattttg gtcagtcact ctccagtcga
    86341 caactgctcg acctggcaaa agacatcacc atcgcctacc gcaccgaatt aaaagacacc
    86401 cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg cgcaggattt tcgtctgcaa
    86461 ctcggtgagc caggttatcg cggtaacctg cgtgagctgt tagctaatcc gcaaattcag
    86521 cgggcatttt tactgctcga tgacaccctg gaactttgtt atgacgtggc gaaactgtca
    86581 ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg ccacgttgta tcgcacacgg
    86641 ctgaagcggc taaaagagat caatcagccg ggctacagct actggtacga atgcacttcg
    86701 cgccatttta ctctggctct cacgccgctc agcgtggcgg ataaattcaa agagttaatg
    86761 gcgcaaaaac ccggtagctg gatcttcacc tcagcaacgc tgtcggtgaa cgacgatctg
    86821 catcatttca cctcgcggct tggcatcgaa caggccgagt cgttgctgtt gcccagccca
    86881 tttgattaca gccgccaggc gttactctgt gtgccgcgca atctgccgca aaccaaccag
    86941 ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga tcatcgaagc taacaacggt
    87001 cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg atctggccga gcagttccgc
    87061 gctaccatga cgcttcctgt attgttgcag ggggaaacca gcaaagggca actgttgcag
    87121 caatttgtca gcgccggtaa tgcgcttctt gtggcaacca gcagtttctg ggaaggggtg
    87181 gacgtgcgtg gcgatacatt gtcattggta attatcgaca aattgccgtt tacctcgccg
    87241 gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt tgcgcggtgg cgacccgttc
    87301 gatgaagtgc aactaccaga tgccgtcatt actctcaaac agggggtagg gcgactgatt
    87361 cgcgacgccg acgatcgtgg cgtgctggtg atttgtgaca atcggctggt gatgcgtcct
    87421 tacggcgcga cgtttctcgc cagtctgccg cccgcgccac gcacccgtga cattgcccgt
    87481 gcggttcgtt tccttgcgat accatcctcc aggtaatttg ttacggagta tggtatgatg
    87541 cgcgcctttt tattgatcta agcacgaaga cccatgcgaa ttctggctat cgataccgcg
    87601 acagaggcct gctctgtcgc cctgtggaac gacggtactg tcaacgctca ttttgagctt
    87661 tgccctcgtg aacatactca acgaatctta ccgatggtgc aggatatcct gaccaccagc
    87721 ggaacttccc tgactgatat taacgctctg gcttacgggc gcggccccgg tagctttact
    87781 ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc ttggcgcgga attaccgatg
    87841 attggcgtct ccacgctaat gacgatggcg caaggggcgt ggcgcaaaaa cggcgcaacc
    87901 cgcgtgctgg cagccattga cgcgcgaatg ggcgaagttt actgggccga atatcagcgt
    87961 gatgaaaacg gtatctggca cggtgaagaa accgaagccg tactcaaacc cgaaatcgtc
    88021 catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg tagggacggg ctggcaagct
    88081 tggccggatc tcggtaaaga gagcgggctg gttttgcgcg atggcgaagt gttactgcct
    88141 gctgctgaag atatgctgcc gattgcgtgt cagatgtttg ctgagggtaa aacggtggcg
    88201 gtggaacatg ccgaaccggt ttatttacgt aacaacgtcg catggaagaa acttccgggc
    88261 aaagaatgaa tcttaagagt atgccctgag aaaaaaagga gtcgcaccat ggcggttcaa
    88321 aagaatgtta tcaaaggcat actggcaggt acgtttgcgc taatgctgag cggttgtgtc
    88381 actgtgccgg acgccattaa aggcagcagt cccacgccgc aacaagattt agttcgggtg
    88441 atgagtgcgc cgcagctgta cgttggtcag gaggcacgct ttggtggcaa agtggttgcg
    88501 gtacaaaacc agcaagggaa aactcgcctg gaaattgcta ccgtaccgct ggacagcgga
    88561 gccagaccga cgctgggaga accttctcgc ggtcgcattt atgccgatgt gaacggtttt
    88621 ctggacccgg tggatttccg tggacaactg gttacggtag tcgggccaat cactggtgcg
    88681 gttgacggca aaatcggcaa tacgccctat aaatttatgg tgatgcaagt aacgggttac
    88741 aaacgttggc atttaaccca gcaggtgatt atgccgcctc agccgattga tccatggttt
    88801 tatggcggtc gtggctggcc ctatggctac ggcggatggg gctggtataa tcccggcccc
    88861 gcgagagtac aaacagttgt aactgaataa ttgcttgttt ttaaagaaaa agaaacagcg
    88921 gctggtccgc tgtttctgca ttcttacggt aaagataaaa ataaatagtg acgcgcttcg
    88981 caaccttttc gttgggtaat tatcaagctg gtatgatgag ttaatattat gttaacggca
    89041 tgtatatcat ttggggttgc gatgacgacg aacacgcatt ttagaggtga agaattgaag
    89101 aaggtttggc ttaaccgtta tcccgcggac gttccgacgg agatcaaccc tgaccgttat
    89161 caatctctgg tagatatgtt tgagcagtcg gtcgcgcgct acgccgatca acctgcgttt
    89221 gtgaatatgg gggaggtaat gaccttccgc aagctggaag aacgcagtcg cgcgtttgcc
    89281 gcttatttgc aacaagggtt ggggctgaag aaaggcgatc gcgttgcgtt gatgatgcct
    89341 aatttattgc aatatccggt ggcgctgttt ggcattttgc gtgccgggat gatcgtcgta
    89401 aacgttaacc cgttgtatac cccgcgtgag cttgagcatc agcttaacga tagcggcgca
    89461 tcggcgattg ttatcgtgtc taactttgct cacacactgg aaaaagtggt tgataaaacc
    89521 gccgttcagc acgtaattct gacccgtatg ggcgatcagc tatctacggc aaaaggcacg
    89581 gtagtcaatt tcgttgttaa atacatcaag cgtttggtgc cgaaatacca tctgccagat
    89641 gccatttcat ttcgtagcgc actgcataac ggctaccgga tgcagtacgt caaacccgaa
    89701 ctggtgccgg aagatttagc ttttctgcaa tacaccggcg gcaccactgg tgtggcgaaa
    89761 ggcgcgatgc tgactcaccg caatatgctg gcgaacctgg aacaggttaa cgcgacctat
    89821 ggtccgctgt tgcatccggg caaagagctg gtggtgacgg cgctgccgct gtatcacatt
    89881 tttgccctga ccattaactg cctgctgttt atcgaactgg gtgggcagaa cctgcttatc
    89941 actaacccgc gcgatattcc agggttggta aaagagttag cgaaatatcc gtttaccgct
    90001 atcacgggcg ttaacacctt gttcaatgcg ttgctgaaca ataaagagtt ccagcagctg
    90061 gatttctcca gtctgcatct ttccgcaggc ggtgggatgc cagtgcagca agtggtggca
    90121 gagcgttggg tgaaactgac cggacagtat ctgctggaag gctatggcct taccgagtgt
    90181 gcgccgctgg tcagcgttaa cccatatgat attgattatc atagtggtag catcggtttg
    90241 ccggtgccgt cgacggaagc caaactggtg gatgatgatg ataatgaagt accaccaggt
    90301 caaccgggtg agctttgtgt caaaggaccg caggtgatgc tgggttactg gcagcgtccc
    90361 gatgctaccg atgaaatcat caaaaatggc tggttacaca ccggcgacat cgcggtaatg
    90421 gatgaagaag gattcctgcg cattgtcgat cgtaaaaaag acatgattct ggtttccggt
    90481 tttaacgtct atcccaacga gattgaagat gtcgtcatgc agcatcctgg cgtacaggaa
    90541 gtcgcggctg ttggcgtacc ttccggctcc agtggtgaag cggtgaaaat cttcgtagtg
    90601 aaaaaagatc catcgcttac cgaagagtca ctggtgactt tttgccgccg tcagctcacg
    90661 ggatacaaag taccgaagct ggtggagttt cgtgatgagt taccgaaatc taacgtcgga
    90721 aaaattttgc gacgagaatt acgtgacgaa gcgcgcggca aagtggacaa taaagcctga
    90781 gcgttaagtc agtcgtcaga cgccggttaa tccggcgttt tttttgacgc ccactaaaga
    90841 gaaaacaatt tgaattacca aatgattacc acggacgatg cgctggcttc tttgtgtgaa
    90901 gccgtccgtg cctttccggc gatagccctg gatactgaat ttgttcgtac gcgcacttat
    90961 tacccgcagc tggggttgat tcaacttttc gatggcgagc atctggcgct aatcgatcca
    91021 ctcgggatca ccgactggtc accgctgaaa gcgatcctgc gcgatccgtc catcacaaaa
    91081 tttctccatg caggcagtga agatctggaa gtgttcctca atgtctttgg cgaattacca
    91141 caacccttga ttgacacgca aatccttgct gccttctgcg gacgcccgat gtcatggggt
    91201 ttcgcttcca tggtggaaga gtattccggc gttacgctgg acaagagtga atcgcgcacc
    91261 gactggctgg ccagaccgct gaccgaacgt cagtgtgaat acgcagcggc ggatgtctgg
    91321 tatctgttac cgatcaccgc caagcttatg gtagaaacgg aggcctccgg ctggctacct
    91381 gcggcgctgg atgaatgccg cctgatgcaa atgcgtcgtc aggaagtcgt tgcgccggaa
    91441 gatgcctggc gtgatatcac caatgcctgg caattacgca cacgccaact ggcctgtctg
    91501 caactgttag ccgactggcg actgcgcaag gcgcgagagc gcgatctggc ggtgaacttt
    91561 gtcgtgcgtg aagagcattt gtggtcggta gcgcgttata tgccgggaag tttaggcgaa
    91621 ctggacagcc tgggtttatc cggtagcgaa atccgctttc acggtaaaac gctgctagcg
    91681 ctggtggaaa aagcgcagac attgccggaa gatgccttac cgcagccgat gcttaacctg
    91741 atggacatgc cgggttatcg taaagcgttt aaagcgatta agtcgctgat tactgacgtg
    91801 agcgaaacgc ataagatcag cgccgaattg ctggcatcgc gtcggcaaat caaccaactg
    91861 ctgaactggc actggaaact gaaaccgcag aacaatttgc cggagctgat ttccggctgg
    91921 cgtggtgagc tgatggcgga agcattacac aatttattgc aggaatatcc gcagtaaaat
    91981 cttccgaagc cggactgggc gcgctcagcg ccacatccgg cttcggcaaa ctacaaatcc
    92041 aacaccaggc gtttaccctt cgcacgcgaa caacagatca acatactttg ctggctggca
    92101 cgctcttcat cgctaaaata ttgatcccga tggtcagctt caccttccag tattgctgtt
    92161 tcgcaggttc cgcatacccc ttcacgacat aaacattcca ctttcgcggc tttattattt
    92221 tcaataacct gcaaaatagt catctcttcc ggcaccacaa actcttttcc ggaacgggca
    92281 agcaccaggg taaatgcatc gccggttttg tcttcgatag caaattgctc aaagtgcagc
    92341 gtatcggcgg cgatgtccag acgcgcagct tcacttctta ccgcttcaat tagcgcctcg
    92401 gggccacagg tataaacgtg tgtgccaggt tcgatatccg ccaataatcg cgctaattcc
    92461 agtcgtgttc cggttgatga atgatgcaaa tggactttct cagcctgcgg atgctggact
    92521 agctcatcac gaaatgcgca actttctgga tttcgcgagc agtaatgtag ctgccagtcg
    92581 acgtcgctgt gttgcagctc tgccatgtgc gacaggaaag gggtgatacc aataccgccc
    92641 gcgataaaca gatgctttct ggctgaggga attagcgcaa acaggttatt aggcgttgaa
    92701 atcgttaacc gatcgcccac ttttacctgc tgatgcaaaa agcgggaacc gccgcgcgag
    92761 ttttcctcca gccgaacggc aatctgataa caagaggtgt catgcggcga actcagtagt
    92821 gaatacgcat tgctgtactg gttatcacca tcgctcatct gcacaatgac gtgacttcct
    92881 ccggtaaacg caggtaatgg tttgccatcg gttgccacca gcgtgaagcg tttcacctgt
    92941 tcggtaaggg gttcaacctg gctcacctgt acttcaaaca tttgatagtc tgacattgct
    93001 gtactccatc aaaaatcagc ccagcggcgg aaaataccgc cgccgatgtc attagtcctt
    93061 aaacacctgc gccagcagat tatggaaatg ggcgatacca tgttcggaaa tgccactacc
    93121 gctactgtcg gccatgatgc gcccctgacc acgatagcca cgcgatttca gccctttctg
    93181 tacgctttca accagacgta aatcttccgg acggaacata tcgcgatacc actcaatcag
    93241 cgatttttgc tcatcggtta actcttcatt ggtgaagtaa atatcgtagt tttgcagggt
    93301 agtttcagaa tccaccggga attcataaat gaccgtcatc atccctttga tcggggtgac
    93361 gttcagcatc gtgcacggcc acagccagaa accgtggaat gccgcatccg taccctcttc
    93421 aaatttaaac gactgttcgg acggtttggc gaaaccgtat tgcagcgtcc agttaccgtg
    93481 catggtgtgc cagtaacgat caacctgtac ggagtcggag aaacctggat gcgccggacc
    93541 acagtgatag cactcgagat agttatcgac aatgttcttc cagttggcag gcgtgcgggt
    93601 ggtaaagcgg gccgccagtt tcagatcgtg gacttccggg caggcttcca gcactttcgc
    93661 gcccaggccg ggtaattgat cttctacgct ggtggcgttg gggtccatgt tgatgaagac
    93721 gaatccggca tattcttcca gacgcaccgg aaccagttgc gctttgtcgc tatcgaaatt
    93781 ggcgacgttt tcgcagttac gtgcatgggc caggttgcca tcgagtttga atgcccatgc
    93841 gtgatacggg caggtaatca cattttttgc ttttccttca ccgctcaaca actgatgacc
    93901 acggtgcgga cacacgttat agaacgcgcg caaaacctta tcacgaccgc gtaccagcac
    93961 gatgctttcg ccaatgatct cacgcgtcac ataatcattg gcattcgcca gttcgctgct
    94021 gtgagcgacg caaatccagc ttttggcgaa gacgttctct ttttcgtgtt caaacgcgtt
    94081 ctgatcggta taaaaacggg caggaatggt ccacgcctct tgcgggttag cgcaaaaatt
    94141 ttcgggtagt acaaagtcag ggctcagatt gctcataggg atacctttat cgttaggata
    94201 atggcgctca gttgtcgcct ttgaatacgg ttcccgcagg gagcactggc gttttttcca
    94261 gggcttcaac cggtgtctgc gggagataat gttcaacttg atgagccgga atgtcggcgt
    94321 aatcctgttt cagccagcga ataaacccgc cgactttcac cagtaaaatg acgaggaagg
    94381 gaagggctgt gagcacgacg gtggttttca tcgtttccag cgaagcaccg gtaaacaaga
    94441 tggaaagcgg gatcagagtg atcaccacgc accagaaaag acgcagccca cggtcaggat
    94501 cgtcaccttc ctggagatta cgcgtactgg tcgccgccat ggtgtaggcc actgcatcca
    94561 tatgcgaggc aaggaaaata atcatcacgc ccaggtatgc ggcgaggaac aatttaccgg
    94621 ctggcaacga catcagaact tgctgtacag ctgtttcgcc gcccagtgtt tccagcacct
    94681 gtgggacgtt gattacgcca ttgataaact gatgaatggc atagctttcc attacgccaa
    94741 agaagaacca gcaaccgacg gtgctgccga ggatcagtcc ccagataact tctttaatct
    94801 tacgaccgcg ggaaacgcgg gtgacaaaca ttgctacgcc aggggtgtat gagatccacc
    94861 acagccagta gaaaacggtc cagttgcggg taaatgaacc atcgccaagc ggatcggtga
    94921 ataagctcat ttgcaggaag ttttgcgtgg tcaggccgat ggcattgatg atgctgttgg
    94981 taataaattc ggttgggccg acaatcagca ccagtaatgg cagcaggaac gcgccccagc
    95041 caaccatttt gctcagacgt tgcaaaccgt tgttgatacc aatccacgag cttaggcaaa
    95101 aaatgccgcc ggaaagcagg atcacaaatg cctgcacggt gaagttatcg ggtaaaccgg
    95161 tcagcgcgga aagcccacgg gtaaaggttg ctgcggtaac aacaagggaa atggtcagtg
    95221 cgccgacagt ggcgatcagg aacatcaaat cgaccagttt tccccatggg ccttgcgggc
    95281 gaacgccggt aatagcagca ataatgccgg aaaggctcag acctttgttt ttccgcacat
    95341 gaaagtgata agccatgatt aatgaggcca gcgtataagt tgcccaggcg ctgatgcccc
    95401 agtggaagaa agagtaggga acgctaaatt cgagtgcctg ttgtgaacgc ggtgcgatat
    95461 ttaatccagg tgtttgataa taataggccc attcagcaac cccccaataa agcgtagaag
    95521 aacctaaacc ggcacaaata aacataaaca gccaggagag cgtgctgtat tccggttttc
    95581 cttcgccaag acgaatattg ccgtatttac tggtggctaa ataaatcacc agtcccattg
    95641 ccagcaaaac caatacctgt acggcggaac ctaacgtacg cgtaacaaaa tttgcatgtt
    95701 acataaatta aacaattaag aatacggaaa gccataaaac acgaaacgga aacaataaaa
    95761 ggtgaaatga cgcaggttat ttactgcaaa gaacgaataa aaaaaccgcg tcagtgacgc
    95821 ggttcaaata aactggtttg accttaacgc aaaataattt tgcaaatcgc gtcggcaacc
    95881 tgtggcgtgg tggcattgcc tttcatatca ggtgttttcg gcccgtgagc aatcacttct
    95941 tcaattgctg ccagaatacc gttatgcgct tgctggaaac gctcatcgcc attgccgaga
    96001 aaatcgagca tcattgcccc ggcccaaatc gtggcgatag ggttagcaat atttttcccg
    96061 tagatatccg gcgcggaacc gtggacaggc tcgaagagcg acgggaaagt gcgttccgga
    96121 ttcaggttgg cggatggggc aatgccaatg gtgccggtgc aggccgggcc aagatcggaa
    96181 aggatatcgc caaacaaatt ggacgccacc accacatcga atcgttccgg ctgcatcaca
    96241 aaacgcgcgc agagaatatc aatatgctgc ttgtcccagc ggatctccgg gtaattctcg
    96301 gccattgctt ccactcgctc atcccagtac ggcatgctga tggctaaacc gttcgattta
    96361 gtggcagaag ttagtgtctt acgtggacgg ctttgcgcaa gttcgaaggc ataacgcaaa
    96421 atgcgatcga caccacggcg ggtaaatacc gattcctgaa tgacgacttc atgctctgta
    96481 ccttcattca ctctaccgcc gagcgaggaa tattcgcctt cggtgttttc cctgaccacg
    96541 taaaaatcga tgtcgccagg ctgttttccc gccagcgggc agggaacgcc aggaaagaga
    96601 cgaaccgggc gcaggttgac gtactggtcg aattcacgac gaaatttcag cagcgaaccc
    96661 cacaacgaaa tatggtccgg aacggtatcc ggccagccga cggcaccaaa atagatggcg
    96721 tcgaaacggc taagttgctc atgccagtcg tccggcatca ttttaccgtg atggctgtaa
    96781 tactcgcagc tcgcccactc catttgctca aaactcaagg cgaagcccca gcgctcagcg
    96841 gcagcctgta acacgcgaat cccttcagga aggacttctt tgccaatccc gtctcccggg
    96901 atcgcagcaa tacgcatcgt tttcatcatt gggttctcac ttcctgacca tcaaaattga
    96961 caacatcacc atcctataat tgactgaaaa ggaattaatc cccgtaactg gtgaaacata
    97021 aaacacgaat catgaataat ttaccgctgc tgaatgattt gcgcgtcttt atgctggtgg
    97081 ctcgccgggc tggttttgcc gccgtggcgg aagaactggg cgtttcaccg gcgttcgtca
    97141 gcaagcgcat cgccttgctg gagcaaacgc taaacgtggt gttgttacac cgcaccaccc
    97201 gccgcgtcac cattaccgaa gaaggagagc ggatttatga atgggcgcag cggattttgc
    97261 aggatgtcgg tcagatgatg gacgaacttt ctgacgtgcg tcaggtgccg caggggatgc
    97321 tgcgtattat cagcagcttt ggttttggac gacaggtggt ggctccggcg ttatcagcgc
    97381 tggcgaaggc gtatccgcaa ctggaattac gttttgatgt ggaagaccga ctggtcgatt
    97441 tggtcaatga aggtgtcgat ctcgatattc gcattggcga tgacatcgcc cctaatttaa
    97501 tcgcccgcaa actggcgacc aattaccgta ttctctgcgc gtcgccggaa tttattgcgc
    97561 aacatggcgc gccgaaacat ttaaccgatc tttctgcatt gccgtgcctg gtcattaaag
    97621 agcgcgatca tccttttggt gtctggcaat tacgcaataa agaaggcccc catgcgatta
    97681 aagtcaccgg cccactgtcg tctaatcatg gagaaattgt ccatcagtgg tgtctcgatg
    97741 ggcaggggat tgctctgcgt tcctggtggg atgttagcga aaacattgcc agtggtcatt
    97801 tagtgcaagt gctaccggaa tattaccagc cagcgaacgt ctgggccgtt tatgtttcaa
    97861 ggctggcgac gtcagcgaaa gtgcggataa cggtagaatt tttacgccag tattttgccg
    97921 agcactaccc gaatttttca ctggagcatg cctgattaat gattcaatta tcgggttgat
    97981 atcaggttaa aacctgattt tctcctttct aagccgctac agattggtta gcatattcac
    98041 ctttaatcgc gcatgatcga aagataatta aagaggttaa tgtgttcgct gaatacgggg
    98101 ttctgaatta ctggacctat ctggttgggg ccatttttat tgtgttggtg ccagggccaa
    98161 ataccctgtt tgtactcaaa aatagcgtca gtagcggtat gaaaggcggt tatcttgcgg
    98221 cctgcggtgt atttattggc gatgcggtat tgatgtttct ggcatgggct ggagtggcga
    98281 cattaattaa gaccaccccg atattattca acattgtacg ttatcttggt gcgttttatt
    98341 tgctctatct ggggagtaaa attctttacg cgaccctgaa gggtaaaaat agcgaggcca
    98401 aatccgatga gccccaatac ggtgctattt ttaaacgcgc gttaattttg agcctgacta
    98461 atccgaaagc cattttgttc tatgtgtcgt ttttcgtaca gtttatcgat gttaatgccc
    98521 cacatacggg aatttcattc tttattctgg cggcgacgct ggaactggtg agtttctgct
    98581 atttgagctt cctgattata tctggtgctt ttgtcacgca gtacatacgt accaaaaaga
    98641 aactggctaa agttggcaac tcactgattg gtttgatgtt cgtgggtttc gctgcccgac
    98701 tggcgacgct gcaatcctga tgctttcagc ccgcgttgtc gcgggcttcc catctataat
    98761 cctccctgat tcttcgctga tatggtgcta aaaagtaacc aataaatggt atttaaaatg
    98821 caaattatca ggcgtaccct gaaacggctg gaataaaccg ttttcagcgc attcaccgaa
    98881 ggagggaaaa ggatgcttca aatcccacag aattatattc atacgcgctc aacgcctttc
    98941 tggaataaac aaactgcacc tgccggaata ttcgaacgtc atcttgataa aggaacgcgc
    99001 ccgggggttt acccacgcct ttccgttatg catggggcgg tcaaatatct cggctacgct
    99061 gatgaacaca gtgcagagcc tgatcaggtg atccttatcg aagcggggca gtttgcggtg
    99121 ttccctccag aaaagtggca caacattgaa gccatgactg acgatactta tttcaacatt
    99181 gacttcttcg tggctcctga agtcctgatg gaaggtgcgc aacaacggaa agtcattcat
    99241 aacgggaaat gaatcatggg caaagcaacg tataccgtga ccgtcaccaa taacagcaat
    99301 ggcgtttctg tcgattatga aacagagacg ccgatgactt tgctggtgcc agaagtggcg
    99361 gctgaagtga taaaagatct ggtgaatacc gtacgttctt atgacacgga aaacgaacat
    99421 gatgtttgtg gttggtaatt atcactgatc gttgatggcg caatttctgc gccatcgcaa
    99481 aaaaaacctc cgtgatgctc gtcacaaatc ttgccctccc gatgttgtgc tatgcttttt
    99541 atcagcgact aacccgctaa tgcaaaacag ataagtgagg aaacaatggg cattctgtca
    99601 tggattattt ttgggcttat tgccggtatt ctggcgaagt ggatcatgcc aggtaaagat
    99661 ggaggtggat tctttatgac tatcctgctg gggatagtcg gtgccgtagt cggcggatgg
    99721 atcagcacgc tgtttggctt tggtaaagtc gatggcttca attttggcag cttcgtggtt
    99781 gccgttattg gtgcgattgt cgtgctattt atctacagga agattaaaag ttaacgctta
    99841 aattgcacaa aggctgcaca caggcagcct ttgctatttt ttagaggtga cgttaccacc
    99901 atcccagctc agtgccgagt acgacaataa tcgccacacc catcataata atcgttgttt
    99961 ttttcatctt tatgctcccg gggcagcata gccgccaata aaaaaccatg ctaaaaatac
   100021 gaccgctgta atgaagatta caaccggaaa aataataccg attcgcatgt tatcaccaat
   100081 caaaaggatg tgaatacgcc tcaggaatgt agcgctggat gcgcgacaaa aggtgttttc
   100141 tgtttgtgtt ttttctcctg atacatcgca atatcggcag catgcagggc actgtctgca
   100201 tcggttgttt caggatcgac ttctactatg ccaagactgg caccgggata atacaaatca
   100261 acatcaccta agtgatattc tccacgtatt tgctgctgaa tgcgttctcg cagcgacgaa
   100321 atatccgcat tctcgttgtt tagtgaaaca actaaaaact catcaccgcc gagacgacca
   100381 ataacttcgc cattttgctg gagcgtatta aggcgctcgc caacctgaat gagaaacaga
   100441 tcgccactat tatgaccaaa acgatcattg attaatttga agttatccag atcgataaac
   100501 gcgatcatta tcttatggtt aagatgccgg gcgagggaaa acagtgtcgt cagattttca
   100561 aaaatcgccc gccgattcgg tagcccggtt aacgagtcgg tatacgattg cgcaatcagc
   100621 gcagcattgg cttcgcgcag ctgttcaacc agtgcctctt tttgaatata ttgtgcaatc
   100681 agtccggcga ataactgtaa aacctgttcc gcgcgttcac tccactggcg cttctcactg
   100741 ctggcggcgc aaagcgtgcc atagaatgat ccatccggta agtgaattgg cgtgctcaga
   100801 aatgtggtga tgccaagatt gcgtgccgca atacagtcac cccaacggtc ggggacttca
   100861 tcgctgaaaa agcagttttc atcaatggcg cgtttgcata acgagtaatc ccacgagacg
   100921 gtaaaattct ccgggatgta cattttctgg ctgttacggg caaacataat atgctgcagg
   100981 cgcgcttcga catccacttt ggtcaggtag gttgattcca tatcagtgac catttccagc
   101041 atctccagaa gctgtcggac cagactttcc agtgactgtt ctttggcaag ggattgcgag
   101101 acgcgggcga taatctgatc tgacacggaa caactccagg atacggcggg taaatgctga
   101161 ttatttaatc tttgcaggca tgataaaaca tcatcaacaa agatgaaaca agacgtaata
   101221 tgttttgagt gaatgtaata aaaatagtgc tgcaaaacaa aacgataccg cgatactcta
   101281 atcttggata aatatgtgag atcaataaat gaaaattatc agttttgttc tgccttgctt
   101341 gctggtcctg gctggttgtt caaccccttc tcagccagaa gcacctaaac cgccgcagat
   101401 tggtatggca aatccggcgt cggtctattg ccagcagaag ggcgggacgc tcattccagt
   101461 gcagacagcg caaggggtca gcaacaattg caaattaccg ggcggtgaaa cgattgatga
   101521 atgggcattg tggcggcgtg accatccggc tggtgaaaaa taaccgccgg atgctgtact
   101581 aaaatcacaa gctacgtagc cagtcggcca gcaccagcgc atggttctgc gtggtgtttt
   101641 ttgctgagta gagcagggtc agcggctgtt ttttggcgat gtccgccagc cgctttcctt
   101701 cttgctcgtg ttgcgccagt tctgcaagat attgctcgcg aaaggttgca taatcgacga
   101761 cttcgccgtg aaaggctttg cgcagttccg ttgacggcgt gatttcttta tcccactcat
   101821 caagggctaa atcggttttt ttgataccgc gcggccagag gcggtcgacc agtatgcgat
   101881 aaccatcgct ctgttcagcc ggatcataaa cgcgtttgca ctgaatattc atcgtgtttg
   101941 ttctcccttt cacgcatgat ttgttacgtc actatagcac cggatattag cgaatttctt
   102001 tgtctctccc ggcgcaaagt ccgaaaatcg ccatcagtag tgaaagtatg gcaacaccca
   102061 taagtggtac agaccagtta ccgttagcat cgtgtatttt acccatcagc ggcggcccac
   102121 aggctgccaa caaatacccg acggattgtg ccatccccga gagtgccgcc gcctgatgcg
   102181 cagaactagc ccgcagacca atgaacgtca accccagtat cattgttgcg ccggaaccaa
   102241 aaccgaaaag cagagtccag gtgatcgcgt gcgctggcat aaagcagagc ccaaccgcgc
   102301 ccactgcgca catcaaggca acgaacgctg caataccacg ctgatctttc acatgatgta
   102361 agaaaagtgg gatcagcaaa ccgggtgctg ctgtggctag ttgcagcaaa ccatgcagtg
   102421 aacccgcctg tgcttcgcta tagccgtgac tgatgaggat cgccggaagc cagccaataa
   102481 tcacgtaata gaccagtgag ttgatcccaa gaaacaatgt gacctgccag gcaagcggcg
   102541 aacgccagat accccgagta tgtaaggcgc gcgaggtact caaatttgca tgttgttgac
   102601 ttcgccactg tggcagccat aaaaaaagag ccagcagagg aaaacacatc agcatgagca
   102661 acgcgccttg ccagccaaaa ccgttcaaag ccagcggcac gaccatagcc gatcccagtg
   102721 ccgctgcagc tcccattgtc agggaatatg cgccggtaag tctggcgacg gaatgaggga
   102781 aatcgcgttt aattaatcct ggcagtaaga cattgcctaa tgcaatccca ccgccaatga
   102841 ccgctgtacc gccaaataat aagtaaggcg aagggagaga gcgaattgcg ataccagcac
   102901 agatcaaaag taacgcggca aacaggctac gttccatacc aaaacgtcgc gctaccgggg
   102961 cagccaaagg tgagattagc gcaaaggcca ataatggcag ggtggtcaat aagccggttt
   103021 gcgctgtcgt cagcgagtaa gcggaacgaa tcgtatccag taacggtgct gcgccggtaa
   103081 aggtgacgcg taatgttgtg gcaatcatca gaatgccagc gataaggaca atcctgtttt
   103141 tgccgcttaa tgaagttgaa caggtcatat gcttctcatt gttacaaacg aggctgtacc
   103201 ttaccgtttt tattgttatg ttgaatcaag ctaaaatgac aaattatcgc taagttcgga
   103261 caaaatgatg cacaggttga atctcaatgg ctatgaaccc gaccgacacc atgaagccgc
   103321 ggtcgcattt tgtattcacg ccggaacgga tgagttaacc agtcctgtac atcagcaccg
   103381 caaaggacag ctgatactgg cgctacatgg tgcaattacc tgtacggtgg aaaatgcttt
   103441 gtggatggtg ccgccacaat atgcggtttg gataccgggc ggggtggaac atagcaatca
   103501 agtaacagct aatgctgaac tctgttttct gtttatcgaa cccagcgcag tgacaatgcc
   103561 gacaacatgt tgcacattga aaatatcgcc cttatgccgg gaattaattt taaccctggc
   103621 aaacaggacg acgactcagc gtgctgagcc aatgacccga agattaattc aggtcctgtt
   103681 tgatgaactc cctcagcagc cacaacaaca gctgcacctg cccgtttctt ctcatcctaa
   103741 aatccgcacg atggtggaga tgatggcgaa agggcctgtc gagtgggggg cattggggca
   103801 atgggctggc ttttttgcga tgagtgaacg caatcttgcc cgactgatag taaaagagac
   103861 ggggttgagt tttcgccagt ggcgtcagca gttacagctg attatggcgc ttcagggatt
   103921 agtgaaaggc gatactgtac agaaagttgc tcatacgctg gggtatgatt caacaacggc
   103981 ctttattact atgtttaaga aaggcctggg gcaaacgcca ggccgatata ttgcgagatt
   104041 aactactgtt tccccacaat cagcgaaacc agacccgcgg caataagcgg cccaaccggt
   104101 acgccgcgaa acagcgctac gcctaaaaca gtgcctacca gcagcccggc gaccagttgc
   104161 ggctggctgc ccattaacgt cacgccgcgc ccacccagcc aggagacaat cacgcctact
   104221 gcaatcgcca ccagtgattt ccagttcaga aacgagtgaa tcaaggttga gggcggtagc
   104281 gtcccgctgg cgatgggggc cataacgcca atcgtcagaa tgattatccc gatacttagc
   104341 ccctgttttt caatccacgg aaaaaaggtg cttaacggtg tgacgcggac aatgattaac
   104401 accagaattg aaacggcgac agtggtgtta tgactgataa agcccagcgc agctaacccg
   104461 agcaggatca gcaaagtgac atcaaacatc gtggtttcct tgccaaaaaa taataagcct
   104521 gcttacttta cgcttgctgc aggaaggaag tagaaaatct ttacgttttt tgcataaaaa
   104581 atcaggcgaa ctgtcatcaa actgccattt gtgcacgcta acaataacgt gaaaacagct
   104641 atctcccggg ggcatgctat gaacgatcaa atgtttgtcg agacaatgat tatcacgtca
   104701 tcgttttttg ccattgctgt tgtactggtt ttgtccgttc ttcttatcga acggaccggc
   104761 tagttacgca gtgcggcgga aattgaccag ttctggttgc gcaatgcgca aataatccgc
   104821 ggttttcaaa ataacggatt tatccagcat gcctgcattg aaggcgattt catcaaatcg
   104881 ttcgaagagt agagggtcgg caaccagttt gagttttgga tggaagctga aaggggggat
   104941 cgcgccgaag acacagcctg tgagttcatc gacttccgcc gggctggcca acgaagcgcg
   105001 taatccgcct atatggctgg cgagttggct cagatcggcc tgttgatcgg ctgcgaggat
   105061 cgccagaaca tgttgattta cgccattgcc tttgacttta cataccagtg ccttagcacc
   105121 ctggcctaga gaggtgccac gaatttctga caccgcttca catttaccta ccgcttcatg
   105181 ggtaacgacg cggaagtcag caccttcttg tgataataaa gcgattaacc gctgatgcgt
   105241 cacgcttcct ttagccattt cagtcatttc acgctcctga gattacaagc aaacaaccac
   105301 agaaggttat catgaacggc tgtttttgtt ctgcttattg acatataaac gctcatcgga
   105361 agctttatac gcatcatgta acgtatcgtt ttctttcatc gcgtaaatac cggaagagaa
   105421 gccgatctct ttctgcggcg cgatatgttg cagacgtttt tcgatacgtt caggcagttg
   105481 tgctgcaatt tgcggcgtcg aatcgacaag aatgatgcag aattcatcgc caccgagtcg
   105541 gatggcataa tcacttttac gaatcgactg tttaatcgcc tgagctaata acgtaatcgc
   105601 taaatccccc tcctgatgac cgagggtgtc atttatttgc tttaacttgt ccatgtcaat
   105661 agcaataaac atcaccgaag aaccggattg caccagtttc tgcaaccgct gctccagttc
   105721 aggggttaaa attttgcgat tatacagtcc agtcatcgca tcactaatat tttctcgcga
   105781 cacattttgg tataaacgga agtgcatccg caccatattc agcaaaatta acgccgtcaa
   105841 aatccagaac agaacgcttt tccacgaaga cgtgataaag taaagaatat caatggataa
   105901 cgagactcga atgccgcccg gtaagtcatg gacgtaactg acatactgga acagattatc
   105961 ttcgctctgg ttgatgataa tgtcgcgccc cgaatcggta tcggttagcg tgacattgag
   106021 aaaacgccag aggagagggc ggtcatgagt ataaaagata ttccgcagat tgtttttgtt
   106081 aatatccagc agcacaatcc cttttagctg ccctgcgaca tataccggcg tcagcaaact
   106141 catcacgttc tgtttagtac gctgatcctg gtaaatgctc gacaatacgg tacgcccggt
   106201 aaataccttt tcaatatctt tgcgatcgat gccaatactt cctgactgca ggaaggacca
   106261 gttattcatc gcaaagtatt caacattaac cggctcataa aaatagacat agcggtcatt
   106321 gagatcgaga taatagtgaa atttctcttc ggccatccca agacctgaac cgtaatcatc
   106381 ctggtggcgg ttgccttcca ctgcctgatc aaaagcgggt aatagtgctg cagcttccac
   106441 aattgtgtcg cagtcagtgg atgcggtttg tagtgtgccg cgtaagcccg ggtatttatg
   106501 ggcagtcagg tttaagccat gggtgttatt ggcagactca aaagcctcgc agatggcgcg
   106561 ccgctgttct ggcgacactt ccgactgctc agcagcgaga gcgcgcatca catgcgcggc
   106621 aatactctga ttctgatact tatcataaag aaaggaggag tctgctttct gcacgatata
   106681 gcgcagatag gcactcattg ctctgtcgct ggcgacaagt tcaaaaataa ggaaggaaga
   106741 tgtcagcaca atgacacttg cggcgataaa atgccggagc attctatggt gcaatttcat
   106801 gataaatggc actccttcgg ttagtgaaac gagaaaacca gataaagttc gattgcggcc
   106861 tggcacaggg gcgctaatta tactcatctc tgcgccgctg gggaggggaa atttaggggt
   106921 aatagtaata aattgtatgc aaaagccacg caaaaagcat ggctctgaat atttttgcac
   106981 ttaaatatga tgaataatca ctttgttgcc cccctcgctt ttcgcttcgc gtaaggcttt
   107041 atctgcttcc gtcatgacaa gcgagatatt acgcgtgtca cccgttgaaa aaaccgtgcc
   107101 aatactgatg gtcatttgtt caggaacatc atatctgtta ctaaagccgg tcaggagttc
   107161 gacattttta cgcatacgtt ccgcaatgat ttttgcctgg gcactattga gttcggtaaa
   107221 tagcaagcca aacacctcac cctccagtcg cgctaaaata tcatcggggc ggatgctttg
   107281 ctggataata tcgacaattg aaaccagcac tttatcaccc acacgatgtc cccattgggc
   107341 attgatgcgt ttgaactgat taatatcaac cagcatgaca cagaagtcct taacaacagg
   107401 tcgcgacaat aatgactcta cgctgttgaa aaagtagcgc cgattataaa tattggtcag
   107461 cacatcatga actgccagtt tgctggagag ttgcagctcc tgaaaaatgt tataaatcag
   107521 aaaagacaca acaaacagtt tactggaaac ttcaatcccg cgactgatat accagatagt
   107581 ataattatac tcatccagag tcagtagaag taagttgcag catagcgatg ccagacataa
   107641 taatgctatt aacggccata gttcgctggc taatcgtgtg cgcatgataa taaagaacag
   107701 taaaaatgcc cacagacaaa ccaatatttt tgtatagttg attccccagg tcgcagtatg
   107761 gttgtccgga cagtaatcga cgacatacaa agaatagtca gcgttataac tgctcagatt
   107821 gtgtgccaga aggggaaaaa ctaaaaaagg gatcagcgcc agcaacagga ttcccgtttt
   107881 tttcttattg ttgtcaagga tgttgtcttt tccataacaa aagagcgcca gcgaggttaa
   107941 acaaataaaa ctgagttggc ggaaaatata gaaaatagaa acatcattcg actttgtctg
   108001 gatcagtgta ctgccgttaa ttggtttatg gataattaca atggtttcaa ctaaatagat
   108061 aaggccactt aaaaatgcaa ggcttaaaat gagtgttggt aaaatttgtt tgttagtgaa
   108121 atatttcatt aacatgaaaa gaaaaatgac ggcgttaagg aaaaacaaag ccacaacaac
   108181 catcaggtaa ttcatctgtg ggatgagttt tacatttgtg ttttcgttat ataaggtagt
   108241 aatgcaaaat aaaattaacg tcagcaataa actgaaaata aaaaagcttt gagcttttaa
   108301 tttttgtgta cccatagaaa ccataaaaag gagagcaatg tgagtgctgg attttattga
   108361 tggcagagat atatagtttt gaccaggatc aattttcgtt aacgataaaa aatatttaaa
   108421 gaataacccc atggaaatac gtgttgactg aatcattttt ccgaatgatt gttttctgga
   108481 actaaattat gtaaaggtta tgctgtcata tttaaagcga ttgtaagcta atgtatgtaa
   108541 taaatgagat aatttacagt aagctaaaaa tcagccaggc gataatgacc tggctgatag
   108601 ttttagcctt tagctgttgc attttgttta tgaaacagtt cacggaacac cggataaata
   108661 tcatcctggt cgcggatgtg ctgcatcgca aagttgtcga aagtagattg cagatgctca
   108721 tattctcgcc acaatgtctg atgtgcacga cgggtaattt cgatatagct gtaataacga
   108781 acaacaggta ataatttttt cgccaggatt tcatggcaaa gcggagagtc atcggcccag
   108841 ttatcgccgt ccgatgcttg tgcagcgtaa atattccact gtgccgggtt ataacgctct
   108901 ttcactacct catccatcag tttcagggcg ctggaaacaa tggtgccgcc tgtttcctgc
   108961 gagtagaaaa actcatgttc atcgacttct ttcgcctggg tatgatggcg gatgtatacg
   109021 acttccacgt tcttatacgt tctgctgagg aacagataca gcagaatata aaaacgctta
   109081 gccatatctt tagtggattg atccattgaa ccggaaacgt ccatcaggca aaacatcact
   109141 gcctggctgg agggatcggg ccgcttctcg tagttcttgt aacgtaaatc gaaggtgtca
   109201 ataaaaggga cgcgttcaat tttggcacgt aattctgcaa tttctttgcg cagacgttcc
   109261 tcttccagca gttgcgcagg ttcactgttg ctgatgatgg ccaaattctc ttccagtgca
   109321 tgaagttccc gccgcttgcc tgccgtcatg gctgtgcgtc gcgccagtga gttctgcaat
   109381 gaacgcacaa cgctgatatt ggccggaacg ccgttagcgg tataacccgc ccgatgcgtt
   109441 ttatattcgg tcagctggcg ttgttggttt tgtttcagat tcggtaaggc caaatcttca
   109501 aagagcagat caagatactc atctttcgaa atctgaaaga caaattcatc ctgaccttca
   109561 ccatcctggc tggcctggcc ctgaccactg ccggaacctc cgccgccacc ctggggacgt
   109621 tcaattcggt cgttctggac gaaatggtca ttgcccggat gcacgcggtg gcgcagaccg
   109681 ccacgcccct gatgaaacat cggttcgcta atatcttccg tgggaatgga tacggattcg
   109741 ccgctgtcga cgtcagtcac cgaacgctta ttaatggcct cggagatcga ctgtttaatt
   109801 tgcgctttat aacggcgtaa aaaacgctgg cgattcacca tgcttttgtt tttgccgttc
   109861 agacgccggt caataaacca ggtcatatgc cccccgtact acatttgcca actgtatgct
   109921 acgcctgtgt tgccgcttct caggcccacg gttcgctgtc gttgtaggcc cggtaacatg
   109981 tgtaccgggc cagggttgtt aagacgattt acgtacgcgc aaataccatt cgcacagtaa
   110041 acgcacctgt ttacgggtgt agcctttttc catcatacgg tcgacaaagt cgtcgtgttt
   110101 cttctgctcg tcggttgacg ttttggcgtt aaacgagata accggcaaca gctcctcggt
   110161 attggagaac attttcttct cgatgaccgt gcgcagtttt tcatagctgg tccagttcgg
   110221 attgcgtccg ctgttattcg ctctggcgcg cagtacgaag ttaacaatct cgttgcggaa
   110281 atcttttgga ttactgatcc ccgccggttt ctcgattttc tccagctcgg cgttaagaga
   110341 ctcgcggtca aacagctgcc cggtatccgg atcgcgatac tcctgatcct gaatccagaa
   110401 atccgcgtag gtaacataac ggtcgaaaat gttttgccca tattcggaat aggattcaag
   110461 gtaggccgtc tggatctctt tgccgataaa ctcggcatat ttcgggatca gataaccttt
   110521 caggaactcc agatagcgtt ctgcctgctc ttgtgggaac tgctcgcgct caatctgctg
   110581 ttccaggacg tagaacagat ggaccgggtt tgctgccact tctacatgat cgaagttgaa
   110641 cacgcgggag aggatcttaa acgcaaaacg cgtcgacaga ccgttcatcc cttcatcgac
   110701 accggcgtag tcacgatatt cctgatacga cttggctttg ggatcggtgt ctttcagact
   110761 ttcgccatca taaacccgca tctttgaata aatgctggag ttttctggct ctttcaggcg
   110821 cgaaagaatg gaaaaacgtg acagtgtttc gagcgtgcca ggggcgcatg gggcgtgagt
   110881 caattcactg tgattaagca atttctcgta gattttgatc tcttcggaaa tgcgcaagca
   110941 atacggcacc ttcacgatgt aaacacgatc gaggaaggct tcgttgtttt tgttattacg
   111001 gaaagtgacc cattcggact cgttcgagtg tgcgagaata atcccgttga acggcagggc
   111061 ggagatacct tccgtcccgt tgtagttacc ttcctgagtg gcggttaaca agggatgcag
   111121 cactttaatc ggtgctttaa acatctcaac gaattccatg atcccctgat tggcgcggca
   111181 cagcgcaccg gaatagccgt aggcgtccgg gtcattctgc gcgtagtgtt cgagtttacg
   111241 aatatcgact ttcccaacca gcgcggagat gtcctggttg ttctcatcgc cgggttccgt
   111301 tttggcgata gcaatttgtt gcagaattga cggccagacc ttcactaccc ggaacttagt
   111361 gatatcgcca ccaaattcat gcaggcgttt tgccgcccac ggcgacatga tggtgccgag
   111421 ataacggcga ggaatgccat actctttttc cagaatctgc gcatcttcct gcggattgaa
   111481 aagacagaac ggatgatcgt tgaccgggct acgctcaccg ttcgcgctca atacataaat
   111541 cggtacgagc tgcattaatg atttcagtcg ctcagcaagc gatgatttac ccccacccac
   111601 aggccccagc agatacagga tttgtttctt ctcttccagc ccctgagccg cgtgtttcag
   111661 ataagagaca atctgttcaa tcgcgtcttc catgccgtaa aactcttcaa acgccggata
   111721 acgtgcaatg acccggttag aaaagagtcg agaaagtctg ggttcctggg ctgtatcgac
   111781 catgacaggc tcaccgatag ccatcaatag ccgctcagcc gcgttggcat aagcactgcg
   111841 atcttgccga caagtggtaa gaaactcctg cagtgtgaac tcttcgtcct tggcagcttc
   111901 atatcgctgg cgatagtgat cgaatatatt catggtatgc cgtcctttcg ttttttagca
   111961 caggttaaga gccgttcata tgaatagtag aggctcccgg aagaggtaaa ctgaacgacg
   112021 tctcttgcta aaacacagca actctcatgc cattttatcg acgatgagaa caacgtgccc
   112081 ggtgatacac cttcaaaatt aagcgtagat ggcaaatgga aaacttgcct gcacggcttt
   112141 cttaatttca atggcatatc aacaactcat ccataaattt tgcataatta atgtaaagac
   112201 caggctcgcc agtaacgcta aattcatttg gctgtaagcg cggtgtcatc cgcgtcagga
   112261 aaattaaaca gttactttaa aaaatgaaaa cgtaaaaagg ttgggtttcg atgtattgac
   112321 gggtaaactt tgtcgcccgc taaacatttg tttaaggaat gattaattgt gaccaaactc
   112381 aaacttctgg cacttggagt gcttatcgca acgtctgcag gcgtagcgca cgctgaaggt
   112441 aaattttccc tgggcgcagg cgtaggtgtc gttgaacacc catataaaga ttacgatacc
   112501 gatgtttacc cagtaccggt aatcaactat gaaggcgata acttctggtt ccgtggctta
   112561 ggtggtggtt actacctgtg gaatgacgca acggataaac tttcaattac cgcttactgg
   112621 tcgccgcttt acttcaaagc gaaagacagt ggcgatcacc aaatgcgtca cctggatgac
   112681 cgtaagagca ccatgatggc tggtctgtct tatgctcact ttacccagta cggttacctg
   112741 cgtaccaccc tggctggcga taccctggat aacagcaacg gcatcgtctg ggatatggcc
   112801 tggttgtatc gttacaccaa cggtggcctg accgtgactc cgggtattgg tgtgcagtgg
   112861 aacagcgaaa accagaacga atactattat ggcgtatcgc gcaaagagtc cgctcgcagc
   112921 ggtctgcgtg gctataaccc gaacgacagc tggagccctt acctggagct gagcgccagc
   112981 tacaacttcc tcggcgactg gagtgtttac ggtaccgcac gctacacccg tctgtctgat
   113041 gaagttactg acagcccgat ggtggataaa tcctggactg gcctgatttc taccgggatc
   113101 acctacaaat tctgataatg catctttcca gggcattttg tgcataaaaa cagggcgtca
   113161 cgccctgttt tgcattacgg tggtgcaata cctgggagga aacaatgcaa caaaaaatga
   113221 ttcaatttag tggcgatgtc tcactgccag ccgtagggca gggaacatgg tatatgggcg
   113281 aagatgccag tcagcgcaaa acagaagttg ctgcactacg cgcgggcatt gaactcggtt
   113341 taaccctcat tgataccgcc gaaatgtatg ccgatggcgg tgccgaaaag gtggttgggg
   113401 aagcattaac cggtctgcga gagaaggtct ttctcgtctc taaagtctat ccgtggaatg
   113461 ctggcgggca aaaagcgata aatgcatgcg aagccagttt acgccgtctc aatactgatt
   113521 atctcgatct ttacttatta cactggtctg gcagtttcgc ttttgaagag actgtcgcag
   113581 cgatggaaaa attgatcgcc cagggaaaaa tccgccgctg gggcgtttct aaccttgatt
   113641 atgctgatat gcaggaactc tggcagctgc cggggggaaa tcagtgtgcc actaatcagg
   113701 tgctttacca tctcggttca cgaggaattg agtacgatct actcccctgg tgccagcaac
   113761 agcagatgcc ggtgatggct tacagtccgt tagcccaggc cgggcggttg cgcaatggac
   113821 tgttaaaaaa cgcggtagtc aacgaaattg cacatgctca caatatcagc gcggcacaag
   113881 tattgttggc gtgggtgatc agtcatcagg gtgtgatggc gattccaaaa gcggccacga
   113941 ttgcccatgt ccaacaaaat gcggctgtgc ttgaggtcga actttcttca gcggaattag
   114001 ctatgctgga taaggcatat ccggcaccaa aaggaaaaac tgcgctgtat atggtgtgat
   114061 gttgtgtgcc ggatgcattg cacccggcac acaacattaa cgtaaattaa cgtttcgcaa
   114121 cgcgaatgga ttgcgccaga tgtgcaggtt tctctttggt cactttttgc gtttctgaag
   114181 cgtaagccgt ttctacacaa acaaatgttt tgtagccatc atccggcata tcgcccatgc
   114241 taattgaaag cgccggtccc gggttccagc cgacaacgtt cagatgatgc tggtggccta
   114301 cggcgataat acgattcagc gcttcatcat taatcacgct gcaatcttgt ggattcagat
   114361 acacgcgatc ggtacggtca gggaaggtct gaataccatc ggtcagtaca ttttctttcg
   114421 cgtcattcac tttatcaatg aagcgatcgc ccagcccact gacgcttacc ttagcgatat
   114481 cacccacgtt aaagtaggta tgcagggcag aggtggtttc aaattcgcca tgtgattcaa
   114541 gatcgatttc acaagtttta cccacgcgga aatgcgctaa cagcgtaaag tcgtgcggcc
   114601 agaatttttt cgtctcttcg ctttgcgtca attcaaaagt cagcgctacg ccatcagcat
   114661 cttcatgatg tgatttcagc gtccacggca ggttgcgggc aaaaccgtgc gcaggcagac
   114721 cttgttgtgc cgccggacca aaccacggcc agcaaaccgg tacgccaccg cgaatagcga
   114781 cgccattttt gaacggtgtg ttgttgctca accacagaac ttcttcttca cccgcaggtt
   114841 tccacgagag aaggtgtgcg ccctgtaatg caaaagaggc ttttacctgg ggatgatcga
   114901 ccacaatgag gtccagttca tccagtttac gacgggagag gacaggggag atttgttcga
   114961 tgaccggaag ggcaaaaatt ttcttaatca tgacgcagtc ctttaacttc attttatcag
   115021 gtaaaaaaaa gagcgaccga agtcgctctt tttagatcac agtgtcatct caacttattt
   115081 ggagatgtga gcgatcaggt ccagaacttt gttggagtaa ccggtttcgt tgtcgtacca
   115141 ggataccagt ttcacgaagt tgtcgttcag agcgatacca gctttagcat cgaacacgga
   115201 agtgcaaact tcgccgttga aatcggtaga tactacgtca tcttcggtgt agcccagaac
   115261 gcctttcatt tcgccttcag cagcagcttt aacggcagct ttgatctgct cgtaagttgc
   115321 agctttttcc agacgaacgg tcaggtcaac tacagatacg ttcggggtcg gaacgcggaa
   115381 cgccatacca gtcagtttgc cattcagttc tggcagtact ttacctacag ctttagcagc
   115441 accggtagag gacgggatga tgttctggga agcgccgcgg ccgccgcgcc agtctttgtg
   115501 agacgggcca tcaacggttt tctgagtagc ggtagtagcg tgaacggtgg tcatcagacc
   115561 ttcgatgatg ccgaagttat cgttgataac tttagccagc ggagccaggc agttggtggt
   115621 gcaggaagcg ttggaaacga tgtcctggcc agcatatttg tcgaagttag cgcctttaac
   115681 gaacatcgga gtgttgtctt tagacggacc agtcataacc actttcttcg caccagcggt
   115741 gatgtgttta cgagcagttt cgtcagtcag gaacagacca gttgcttcag cgacaacgtc
   115801 aacaccaact tcgtcccatt tcaggttagc cggatcacgt tcagcggtaa cacggatttt
   115861 tttaccgtta acgatcagat gaccgtcttt cacttcaacg gtaccgtcga aacggccgtg
   115921 agtggagtca tatttcagca tgtatgccat gtaatcagcg tctaacaggt cgttgattgc
   115981 aacgatctcg atgtcagaac gtttctgagc agcacggaaa acaatgcgac cgatacggcc
   116041 aaaaccgttg atacctactt tgatagtcat atattccacc agctatttgt tagtgaataa
   116101 aaggttgcct gtaaaattac aaaaacctta cgcagcgtca agcggaatcg tgtcaatcat
   116161 tgcgacaaat caatcctgtg cctaagcatt acgcgactga ctcgcctcac tcttcctttg
   116221 ggcttgagac cacatggggt cggcgccccg aattttaaag ggcaattacg ataaaaatgt
   116281 gattttcatc acgatttagg tgcagccatt tcgtcgagat caagtttagg acaaaatttt
   116341 gccagttggt gttaatgttt tgttagaatc agtcacgtaa tgtgagcacg attaaagtga
   116401 gatgtgagca aatggctaat aaaccttcgg cagaagaact gaaaaaaaat ttgtccgaga
   116461 tgcagtttta cgtgacgcag aatcatggga cagagccgcc atttacgggt cgtttactgc
   116521 ataacaagcg tgacggcgta tatcactgtt tgatctgcga tgccccgctg tttcattccc
   116581 aaaccaagta tgattccggc tgtggctggc ccagtttcta cgaaccggta agtgaagaat
   116641 ccattcgtta tatcaaagac ttgtcacatg gaatgcagcg catagaaatt cgttgcggta
   116701 actgtgatgc ccatctgggg catgtcttcc ccgacgggcc gcagccaacg ggcgaacgtt
   116761 attgtgttaa ctctgcctct ttacgcttta ccgatggcga aaacggcgaa gaaatcaacg
   116821 gttgaaaaaa cgattcagca acttattcca caggagcgga aaaattatga atcttgatga
   116881 cattatcaac agcatgatgc ctgaggtata ccagcgtttg tcgaccgccg ttgaactggg
   116941 gaaatggcct gatggcgttg cgttgaccga ggaacaaaaa gagaactgcc tgcaactggt
   117001 gatgctgtgg caagcccgcc ataatatcga agcacagcat atgacaattg acacaaatgg
   117061 tcagatggtc atgaagagca aacagcagtt aaaagaagat tttggtatct cagcaaagcc
   117121 tattgccatg tttaagtaag tggttcatcc cctttttgcc tcggctatga aggggatagc
   117181 caacaatgac cccacgactc atctcaatta tctcgccatg acctggctaa gtcccagtcc
   117241 gatctcttgc cgtcccgttc acacctgaat gacttctcct gaacttaagt ctcagaccta
   117301 tttggccggt aatccctctc gaatttgctc tttttgcgcc ccgccttcca tcaacctgta
   117361 acagacccag ccattttctg cgtaattgtg atctgagttg taaaacgcag atcattatct
   117421 gtgttttaca gatttaatgt tgacctcaaa aatcatgtgg gtaagtatga tggccatcta
   117481 agcaaaacta aacacagagg atgacgcaat gaaagcactg gctcggtttg gcaaggcctt
   117541 tggcggctac aagatgattg atgtcccaca acccatgtgt ggcccggaag atgtagtgat
   117601 tgaaattaaa gccgcggcaa tctgcggcgc agacatgaag cactacaatg tcgatagcgg
   117661 ttctgatgag tttaactcta tccgcggcca tgagttcgca ggttgtattg cgcaggttgg
   117721 tgaaaaagtc aaagactgga aagtggggca acgcgtcgta tcggataaca gcggtcacgt
   117781 ttgcggtgtt tgtccggcct gtgaacaagg tgattttctg tgttgtacag aaaaagtaaa
   117841 ccttggtctg gataataata cctggggcgg tggtttttcc aaatattgtc tggttcctgg
   117901 tgaaattctc aaaattcatc gtcatgcgtt gtgggaaatc cctgatggtg ttgattatga
   117961 ggacgcagcc gtacttgacc ctatctgtaa tgcctacaaa tccatcgcgc agcaatcgaa
   118021 attccttcct ggtcaggatg tggtcgtcat cggcactggc ccactcgggc tgttctccgt
   118081 acaaatggcg cgaattatgg gggcggtaaa tatcgtcgtc gttggtctgc aagaagatgt
   118141 ggcggtccgc ttcccggttg caaaagaact gggtgcgacg gcagtagtaa atggttctac
   118201 cgaagatgtg gtggcgcgct gccagcaaat ttgtggcaaa gacaatctgg gactggtgat
   118261 tgaatgctcc ggtgccaata tcgcactgaa acaagccatc gaaatgctcc gcccgaacgg
   118321 ggaagtggta cgcgttggaa tgggcttcaa acctcttgat ttctcgatta atgacattac
   118381 cgcctggaac aaaagcatca ttgggcatat ggcctatgac tccacctcat ggcgtaacgc
   118441 tatcaggcta ttagccagcg gcgctatcaa agtcaaaccg atgatcacgc atcgtatcgg
   118501 cctgtcgcaa tggcgcgaag ggtttgatgc gatggtcgat aaaaccgcaa tcaaagtgat
   118561 catgacttac gactttgatg aataaccact ctcctgaagg aacgttatcg catggaacag
   118621 ataacaaaac cgcattgtgg tgcacggctg gatcgcttac cggattgccg ctggcattca
   118681 tcaatgtttg ccatcgtcgc gtttggcttg cttgtctgct ggagtaatgc cgttggtggc
   118741 ttgatcctcg cgcagctgaa agcgttgggc tggacagata attccaccac agccacattc
   118801 tcagcaatca cgaccgccgg aatgtttctc ggtgcgctgg tgggcggcat cattggcgac
   118861 aaaaccggtc gcagaaatgc gttcatcctc tatgaggcca ttcatattgc ctcgatggtt
   118921 gtcggtgctt tctcaccgaa tatggatttc cttattgcct gccgttttgt gatgggagtt
   118981 gggctgggag ctttactggt tacgctgttt gctggtttca ccgaatatat gcccggtaga
   119041 aatcgtggaa cgtggtcaag tcgggtttcc tttattggca actggtcata tccgctctgt
   119101 tcattgatag cgatgggact cacgccgctg attagtgcag agtggaactg gcgggtacaa
   119161 ctgcttatcc ctgcaatatt gtcgcttatc gctacggcgc tggcctggcg ctactttcct
   119221 gaatccccgc gctggctgga atcgcgcgga cggtatcagg aagccgagaa agtcatgcgg
   119281 agtatagaag aaggcgtcat acgccagacg ggtaaacctt tgccgcctgt ggttattgct
   119341 gatgacggta aagcgccaca agcggtgccg tattcagcct tactgacagg agtattactg
   119401 aaacgcgtga tattaggctc ttgtgtgctg attgccatga acgttgtgca gtacacacta
   119461 attaactggt tgccaacaat attcatgacc caggggatta atttaaaaga ctcgattgtt
   119521 ttaaatacca tgagtatgtt tggcgcgcca tttggtattt ttattgccat gctggtgatg
   119581 gataaaattc cgcgtaaaac aatgggtgtg gggctattaa tcctgattgc ggtgctcgga
   119641 tatatctatt cactgcaaac cagtatgttg ctcataacgt tgattggttt cttcctgatt
   119701 actttcgtct atatgtacgt ttgctatgcc tcggcagtgt atgtccctga aatctggccg
   119761 acagaggcca aactccgtgg ctccggtctg gcgaatgcgg tagggcgaat cagtggtatt
   119821 gccgcacctt atgccgttgc agtgctgctc agtagttatg gcgttacggg agtctttatt
   119881 cttctggggg cggtttcaat tattgtcgca attgctatcg ccaccattgg aatcgaaacc
   119941 aaaggtgtct ccgttgaaag tttaagtatt gatgcagtag ccaataaata atacgggtgg
   120001 attcaaaatg aaaaattcaa aagcaatatt gcaggtgccg ggcacaatga aaattatttc
   120061 agcagaaata ccagtgccta aagaagatga agttttgatt aaagtagaat atgtcggtat
   120121 ttgtggttca gatgtacatg gttttgaatc aggcccgttt attccgccta aagacccaaa
   120181 tcaagaaatt ggcctgggtc atgaatgcgc cgggacggtt gtggctgtgg gaagccgcgt
   120241 gcgcaaattt aaaccggggg atcgggtaaa tatcgaacct ggcgttcctt gcggtcactg
   120301 tcgttactgt ctggaaggca aatataacat ctgcccggac gttgatttta tggcgacaca
   120361 acccaactac cgcggcgcat taacgcacta tctgtgtcat ccggagagct ttacttacaa
   120421 actgcccgac aatatggaca cgatggaagg ggcgctggtg gagcctgccg cagtcgggat
   120481 gcatgccgcg atgctggcag atgttaaacc gggtaagaag ataattattc tgggagcagg
   120541 ttgtattggt ttgatgacgt tgcaagcgtg caaatgcctg ggagcaacgg aaattgccgt
   120601 cgttgatgtg ctggaaaaac gtctggcaat ggcggaacag cttggtgcga cagtggttat
   120661 taacggcgca aaagaagaca ctattgcacg ctgtcagcaa tttaccgaag acatgggcgc
   120721 agatattgtt ttcgaaacag cgggttctgc ggtcaccgtt aaacaggcac cttatctggt
   120781 aatgcgcggc ggtaaaatta tgattgttgg tactgtaccc ggcgattcgg caatcaattt
   120841 cctcaaaatc aatcgcgaag tcactatcca gacggtattc cgctatgcca atcgttatcc
   120901 ggtcacgatt gaagctattt cttcagggcg attcgatgtg aaatcgatgg tgacgcatat
   120961 ttacgattat cgggatgtac aacaggcatt tgaagagtca gttaacaaca aacgcgacat
   121021 tattaaaggc gttattaaaa ttagcgatta attaatacgg gagaacagaa catgctcgca
   121081 gatatcagat attgggaaaa cgatgccaca aataaacatt atgcaattgc ccattttaac
   121141 gtatggaatg cagaaatgtt gatgggcgtt atcgacgcag ccgaagaagc gaaatccccg
   121201 gttattattt cttttggtac aggttttgtc ggtaacacct catttgaaga tttctctcac
   121261 atgatggtat caatggcaca aaaagcaacg gtgccggtaa taactcattg ggatcatggt
   121321 cggagtatgg agattattca taacgcctgg actcatggta tgaattcatt aatgcgtgat
   121381 gcttccgcgt ttgatttcga agaaaatatt cgtttaacca aagaggctgt cgatttcttc
   121441 catccgctgg gtattccggt agaggcggaa ttagggcatg tcggtaatga aaccgtttat
   121501 gaggaagcgc tggcgggcta tcactatacc gatcctgacc aggcggctga atttgttgaa
   121561 cgtacgggct gtgattcatt ggctgtcgcc atcggcaacc agcatggggt ttatacgtca
   121621 gagccacaat tgaactttga ggtcgtcaaa cgcgtacgcg atgccgtttc tgttccgctg
   121681 gttttgcacg gtgcatcggg gatcagtgat gccgacatta aaactgcaat ttcgttgggt
   121741 atcgcgaaaa tcaacattca tacggagctc tgtcaggccg caatggttgc agtgaaagaa
   121801 aaccaggatc agcctttcct gcatttagaa cgtgaagtac gtaaagctgt aaaagaacgg
   121861 gcattggaaa aaattaaact gttcggttct gatggcaaag cggaataatg atatggataa
   121921 tctcgacgtt atttgtatag gtgccgctat tgttgatatt ccattgcaac cggtcagtaa
   121981 aaatatcttt gatgtggatt cttatcctct tgaaagaatc gcaatgacca ccgggggtga
   122041 tgcaataaat gaagcaacaa ttatttctcg tctgggccat cgcacagcat taatgagtcg
   122101 tattggtaaa gatgccgcag ggcaatttat tctcgaccat tgccgcaaag agaatattga
   122161 tattcaaagc ctgaagcagg acgtcagcat agatacctct attaacgttg gactggtgac
   122221 ggaggatggc gagcggacgt ttgtcacaaa ccgtaatggc agtctgtgga aattgaatat
   122281 tgacgatgtt gattttgcgc ggttttctca ggcgaaatta ttatcactgg ccagtatttt
   122341 caacagtcca ctattggatg gtaaagcgct aacagaaatt tttacgcaag ctaaagcccg
   122401 gcagatgatt atctgtgccg atatgatcaa accgcggttg aatgaaacgc tggatgatat
   122461 ttgcgaagca ttgagttatg tcgattatct gtttcctaat tttgccgagg caaaattact
   122521 caccgggaaa gagacactgg atgaaattgc tgactgcttt cttgcgtgcg gcgtaaaaac
   122581 ggtggtgatt aaaacgggta aagacggctg ctttatcaag cgtggtgaca tgacgatgaa
   122641 ggtgccggcg gtcgcaggaa taaccgccat cgacaccatt ggcgcgggcg ataactttgc
   122701 ttcaggtttt attgcggcac tgttagaagg caaaaatctg cgtgaatgcg cacgctttgc
   122761 caatgcaacg gcggctatct cggttctaag cgtcggtgcc accaccggcg taaaaaacag
   122821 aaagctggtc gaacaattgc tggaagaata cgaaggataa tgaaggcaaa tgaaaaagat
   122881 acctttaggc acaacggata ttacgctttc gcgaatgggg ttggggacat gggccattgg
   122941 cggcggtcct gcatggaatg gcgatctcga tcggcaaata tgtattgata cgattcttga
   123001 agcccatcgt tgtggcatta atctgattga tactgcgcca ggatataact ttggcaatag
   123061 tgaagttatc gtcggtcagg cgttaaaaaa actgccccgt gaacaggttg tagtagaaac
   123121 caaatgcggc attgtctggg aacgaaaagg aagtttattc aacaaagttg gcgatcggca
   123181 gttgtataaa aacctttccc cggaatctat ccgcgaagag gtagcagcga gcttgcaacg
   123241 tctgggtatt gattacatcg atatctacat gacgcactgg cagtcggtgc cgccattttt
   123301 tacgccgatc gctgaaactg tcgcagtgct taatgagtta aagtctgaag ggaaaattcg
   123361 cgctataggc gctgctaacg tcgatgctga ccatatccgc gagtatctgc aatatggtga
   123421 actggatatt attcaggcga aatacagtat cctcgaccgg gcaatggaaa acgaactgct
   123481 gccactatgt cgtgataatg gcattgtggt tcaggtttat tccccgctag agcagggatt
   123541 gttgaccggc accatcactc gtgattacgt tccgggcggc gctcgggcaa ataaagtctg
   123601 gttccagcgt gaaaacatgc tgaaagtgat tgatatgctt gaacagtggc agccactttg
   123661 tgctcgttat cagtgcacaa ttcccactct ggcactggcg tggatattaa aacagagtga
   123721 tttaatctcc attcttagtg gggctactgc accggaacag gtacgcgaaa atgtcgcggc
   123781 actgaatatc aacttatcgg atgcagacgc aacattgatg agggaaatgg cagaggccct
   123841 ggagcgttaa atactcaaca cgattcacct ctgtttctgt gaaaactgtg atgaaaatct
   123901 gtgattcaaa caggttattt tgaaagtaaa catcggttat gcgataatcg cgctaatgtg
   123961 atgtaaaaag atacaggggt gtaatcgtgg cggcaaaaga caggattcag gcaattaagc
   124021 aaatggttgc caacgataaa aaggtgacag tctcaaattt gagtgggatt tttcaggtaa
   124081 ccgaagaaac cattcgccgc gatcttgaga agctggaaga tgaaggcttt ttgaccagaa
   124141 cctatggtgg tgctgtttta aatacagcga tgttgacgga gaatatccat ttttataagc
   124201 gcgcttcatc gttttatgaa gagaagcagc ttattgcacg caaggcacta ccctttatcg
   124261 acaataaaac caccatggca gccgattcca gtagtaccgt aatggaattg ctcaaattgt
   124321 tacaggaccg tagtggcctg acgttgctaa ccaactcggc ggaagcgatt catgttctgg
   124381 ctcagtcaga aattaaagtc gtttcaacgg gtggggaact aaacaaaaat acactttcac
   124441 tgcaaggaag aattactaaa gagatcatca ggcgctatca tgtcgatatc atggtaatga
   124501 gttgtaaagg tcttgatatt aacagcggcg cgctggactc taatgaagcg gaagctgaaa
   124561 tcaaaaagac aatgatccgt caggcgacag aagttgcgtt attggttgat cactctaagt
   124621 ttgatcgcaa agcttttgtc cagttagctg attttagtca tattaattac ataataactg
   124681 ataaatcacc gggtgcagag tggatagcat tttgcaaaga caataatatc cagctggtgt
   124741 ggtaatttaa gcacacgttg tcaatattat ttgttcctgc gccttgaaat aaaatgatga
   124801 taaagatgcg gcaagtgaat ttgccgcaat tgctaccttt ttagcaaatc cgaggcacct
   124861 aatggaacaa tatgatcaaa ttggcgcaag actggaccgc ttgcctttgg cccggtttca
   124921 ttatcgtata tttggtatta taagctttag tctgttatta acagggtttt tgagttactc
   124981 cggtaatgtc gtcttagcaa agctggtaag caatggatgg tcaaataatt tcctcaatgc
   125041 cgcctttacc tcggcattaa tgtttggtta tttcatcggc tcacttactg gtgggtttat
   125101 tggtgactac tttgggcggc gcagggcgtt tcgcataaat cttctcatcg tcggtattgc
   125161 tgcaacaggg gccgcttttg tccctgatat gtactggctc atcttctttc gcttcctgat
   125221 gggaacagga atgggggcgc tgattatggt tggctatgcc tcatttacgg agtttatccc
   125281 cgcgacggtg cgtggaaaat ggtccgcgcg gctctcattt gttagtaact ggtcgcccat
   125341 gctgtctgcg gcgataggcg tggtggttat cgcttttttt agttggcgaa taatgtttct
   125401 gctgggtggt attggcatac tgttagcctg gtttctctca ggtaaatact ttatcgagtc
   125461 gccacgatgg ctggcaggga aagggcaaat cgcaggtgca gaatgccaac ttcgtgaagt
   125521 agagcagcaa attgaaagag agaagagtat tcgtttaccc ccgcttactt cgtatcagag
   125581 caacagcaag gttaaagtaa tcaagggtac tttctggctc ctgtttaaag gtgaaatgtt
   125641 acgacgtaca ttagtcgcga ttactgtttt aattgcaatg aacatttcgc tttataccat
   125701 caccgtatgg ataccgacca tatttgttaa ctccggcatt gatgtcgata aatcaatatt
   125761 aatgaccgct gttattatga ttggcgctcc ggtaggaata tttattgcgg cattaattat
   125821 tgatcatttt cctcgtcggt tatttggctc caccttactt attattattg ccgtgttagg
   125881 ctatatctat tcaattcaga ctacagagtg ggcgatttta atctatggac tggtgatgat
   125941 cttcttttta tacatgtatg tttgcttcgc gtcggcggtt tatatcccgg agctttggcc
   126001 aacgcattta cgcctgcgcg gttcgggttt cgttaatgcc gtcggacgga tcgtcgcagt
   126061 cttcacgccc tatggcgttg cggcattatt aacacattat gggtcgatca cggtgtttat
   126121 ggtacttggt gtcatgttat tgctctgtgc gctggttctc tccatttttg gcatcgaaac
   126181 gcggaaggtg tcgttggaag agatttctga ggtgaattaa atctttcgtt gatggcatca
   126241 attgccagat gtgccactga cgtgttttat ttggcctaca atgccagtac agaattgtag
   126301 gccagcgtaa aattacccct gtgtctcttc ccagtctgcc agcgtatata gcgttgcccc
   126361 agctgctgac atctccataa acgcgtgcgc actgtcctgg ggctggatat tcacgccacg
   126421 acaaccatcg gtaatcacgt ttaccttata acctaactgt aacgcgtcca gcacggtaaa
   126481 cttcacgcaa tagtcagtag ccaggcccat aacgatcaat tcatcgattt catgatcgcg
   126541 taaccagtca tcgagagagg ttttctgccg acggccgtta tcaaaaaagg cactgtaact
   126601 gtcaactaaa ggattttcgc ctttatggaa caccgctgcg atcgcttttt ggtgcagtaa
   126661 cggatgtaat tgtgcgcctt cactgttctg cacacagtga tctggccaga aggtttgtgg
   126721 caaaccgtcg agttggcctg gcgtataagg ctctacaccg tgctgactgg caaaactgcc
   126781 gtgattcgcc gggtgccagt cctgactggc gataaccgct tcaccgcgcg actggcacca
   126841 gtcaatcagg cggttagcga catccaccgt actgtcacct tccggcacgg cgagcgcgcc
   126901 accagcacag aaatcatttt gtaaatcgac cagtaacagg gcgcgagggg gcattacagt
   126961 ctccttaatc atccggcgtc agttcgccgc gcaggttttg gctcatggcc ttgcgaatgg
   127021 tttcagtatc cagttcctgg ctaagcaggt aatgcagttt ggttagcgtg gcttctacag
   127081 tcatatctgc accgccaatt acgccggcat gggcgagggc gttaccggtg gcataaccac
   127141 ccatgttcac tttaccggac atacattgtg tcaggttgac caccacaata ccgcgatcgc
   127201 tggcttcttg taattcctgc aggaaggctt tgttttgtgg cgcattaccc acgccatagg
   127261 agcgcagaat caatgctttc accggttggc gcagaaaatt gcgcacgacg tcagcagaaa
   127321 tccctggata aatcgtcact acgccaattg gttgtggggt gattggatga acgattaatt
   127381 ccccttcacc gtgcggggcg ggtggcgtat tcaaacgacg aatatggata cctgcttcca
   127441 gtaacggagg aaggtttgga gaggcaaacg catcaaaacc atcggcatgg gctttggtag
   127501 tgcggttgcc gcgatacaat cggttattga aaaagagcgt tacttcgttg atcggataat
   127561 tcgccgcaac gtacaacgca ttcagcagat taatttgtcc gtcagagcgt aactcagcca
   127621 gcgggatttg tgaccctgtc acaatgaccg gtttaccgag attctcgagc atgaacgaca
   127681 gcgcagaggc ggtatacgcc atcgtgtcgg tgccgtgcag aatgacaaaa ccatcatagt
   127741 cgtcatagtg cgctttaata tcttcagcaa tatgctgcca gtcttccggc gtcatatctg
   127801 aagaatccat cagcggcgta tattcatgaa tggtgaaatc tggcatctcc gggcgatgga
   127861 attccggcat cagcgccagt tggcgttgta gatgacctga caccggtata taaccctgct
   127921 cggaacgctg catcccgatg gtcccgcccg tgtaggcaac gtaaattgat ttcttttgca
   127981 tgatattgat atgaatgata tcgaaagagc aaaagtatat acgtgaggca gggggagcgc
   128041 acctgatact atgcggataa ggaaatatgg ggcgaaacag cgggaagagg attcgtcgac
   128101 gtaaaaaata atgtggccga cgatctgtcg gccactcagt acaagactta acgcatgttg
   128161 gcgcaggtca ggcaaaacgc ataacggttt tgtgggtcat taaacgcggc cagcttgtca
   128221 ctttcacttt taacggtaga ggctaccgag gccagcggtg caggtaacat ggcctggaac
   128281 gcatctggca acattgcccg gacagaacca gacatgttgt ccatcacttt gtcgaagaag
   128341 gtcggttcat caacgtaata ttccagatgc cactgtttca cttttgccag ctctgctgct
   128401 ttggcgaccg catcatcgaa atccccgaga ctatcgacca gcccgttagc ttttgcatcc
   128461 tgaccggtcc agacgtggcc ctgggcaatt ttatcaatct gctccggcgt cgaatgacgc
   128521 gcatcagcaa ccagcgtgat aaagcgttta tagccattct caatgcttaa ctgcatcatc
   128581 agctgcgctt ccggcggcag tgccctggtg atagaaacat ccgccagcgg tgaagttgag
   128641 acaccatcag tatgaacacc aatcgaatcc agactatttt ctacggtggt gatcacgccg
   128701 aagataccga tagaaccggt cagggtgctg gggttagcca caatgtaatt agctggcgtg
   128761 gaaatccagt aaccaccaga tgccgccatg ccgcccatcg atacaaccac aggcttaccc
   128821 gctgcccggg ctgctgccag ttcagcgcga atcacttcag acgcggtaac gctgccgcct
   128881 gggctattaa cacgcaggac aatcgctttc actttcgggt caaggcgagc gtcgcggatt
   128941 tgtgccgcag tggtatcacc gccaacattc ccctgagttt cctcgccatc cataattgcg
   129001 ccattagcaa agacgacacc gatgctgtca ccggtatctg ccggcgtttt caatgcgtaa
   129061 tcgtaataac tgatggcgcg ataattttta tcagtcttac tccagccgaa ttctttggtc
   129121 agtgcttttt cgatttccgc actcgatgcc agtgcatcga ccagcttgtt ttccagtgca
   129181 tatttcgcgg tatcgccacc ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg
   129241 aatacctgct cagcagggat ctgccggtta gcggcaacag tattcagata gttttgccac
   129301 agctcaccaa tccagcggct gtcagcttcg cgggctgccg gtgacatatc atcacgaata
   129361 aacggttcaa cggcagattt atacgtaccc acgcggaaca catgggtgga aactttcagc
   129421 ttatccagca acgatttgta gtacagaccg ttggtggcaa agccgtgcag atcaaccacg
   129481 ccttgcggag acagccaaat tttattggcg aaactggcga gataatattg cccctggctg
   129541 tagttctcgc caacggcata aaccggtttc ccgctgtcac gaaactcttt cagagctttg
   129601 ccgatgtact gcatagacgg ttggtcgccg cctgcgaagt ttttcagatc catcacaata
   129661 ccggtgatat tgcggtcgtc cttcgcctgg cgaatagtgt tgacgatatc aaacagtgag
   129721 ttttcctgca gacgatcgga actggcacca agcagctggc ggcttaattt actaaaccgc
   129781 tgagaactgt cgggtttatc gacgatcaca ccagaaatgt ccagcagcag tgcgccacga
   129841 ctggccgttt ctttcgaatc accaccactg acctgcatcc aaatccccac accaaccagt
   129901 acgaggaaaa taaagaacag gttaagtacc atttcacgga cgaaattcag cagacgccac
   129961 gtccatttaa aaaatccggc aataaatcgc caaagggttc gcatgtattc tcccaactta
   130021 aggtcacacc tgtcacaggc gcaatagatg tggttatcgt aatgacccaa ccggtacttg
   130081 tcagcaggaa ttgcggactg tgctgtaaca aaatccactc tcgtgttaat tttgtgaata
   130141 aatatcacga caggagttaa tcaaatggat gcactcgaac tattgatcaa tcgccgtagc
   130201 gcctcccgct tggctgaacc cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg
   130261 ggtatgcgtg cgccggacca taagtccatg caaccgtggc atttttttgt gattgaaggg
   130321 gaagggcgcg agcgtttcag cgccgtactg gaacaggggg cgattgctgc cggtagtgat
   130381 gacaaagcta tcgacaaagc ccgtaatgcg ccgttccgcg caccgctcat catcacggtg
   130441 gtggcgaaat gcgaagagaa tcataaagtc ccgcgctggg aacaggaaat gtctgccgga
   130501 tgcgcggtca tggcgatgca aatggcagca gttgcccagg ggtttggcgg catctggcgc
   130561 agtggcgcat taactgaaag tccggtagtg cgtgaagcat tcggttgccg tgagcaggat
   130621 aaaattgtcg gttttctcta cctcggtacg ccgcagctga aagcatctac gtcgattaac
   130681 gtcccggacc cgacgccgtt tgtaacttat ttctgataat tgtcgcgaaa ctgtctggat
   130741 tgtgagcaaa ggcgcaggaa ttcagacact ctcacttatc acttcacgga atgagggtta
   130801 ccatacccgg attgcattga caggagatgt ccatgagcga gaactcgatt cgtttgaccc
   130861 aatacagcca cggagctggt tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc
   130921 tgcatagtga gcaggcgaag tttgttgatc cgaatttgct tgtgggtaat gaaacccgcg
   130981 acgatgcggc ggtgtacgat ctgggcaatg gcaccagcgt tatcagtacc accgacttct
   131041 ttatgccgat cgttgataat cctttcgatt ttggccgcat tgcggcgact aacgccatca
   131101 gcgatatctt cgcgatgggg ggtaaaccga ttatggcgat tgcgatcctc ggctggccga
   131161 ttaacaaact ttccccagaa attgcccgcg aagtgaccga aggtggacgc tatgcatgtc
   131221 gtcaggcggg tattgcgctg gctggcggtc actccatcga tgcgccggag ccgatttttg
   131281 gtctggcggt aacggggatc gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag
   131341 ccggatgcaa actgttcctg acgaaaccgc tggggatcgg cgttcttacc acggctgaga
   131401 aaaaatcact gttgaaacca gaacatcagg gactggcgac ggaagtgatg tgccggatga
   131461 acatcgcagg cgcgtccttt gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg
   131521 gctttggtct gctgggccac ttgagcgaaa tgtgtcaggg ggctggtgtg caggcacgcg
   131581 tcgactatga agcgatcccg aaactccccg gtgttgaaga gtacattaag ttgggcgcag
   131641 tacctggcgg cactgaacgt aactttgcca gctacggtca tctgatgggt gaaatgccgc
   131701 gtgaagtgcg cgatctgctg tgtgatccgc aaacttctgg cggtttgctg ctggcggtca
   131761 tgccggaagc agaaaatgag gtcaaagcta cagccgccga gtttggcatt gaactgacgg
   131821 caattggcga actggtgcca gcgcgcggcg gtcgtgccat ggttgagatt cgttaattca
   131881 atgcggttgt ttattgccga aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc
   131941 aaaccgcacc ggaaaggcga tggctttatc gagtgcggta atggtcaggt ggtgacctgg
   132001 tgtatcggtc acctgcttga gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc
   132061 tggaatcttg cggatttgcc gattgtcccg gaaaagtggc aattacagcc ccgaccctcc
   132121 gtgaccaaac aacttaacgt catcaaacgg ttcctgcatg aagccagcga aatcgttcac
   132181 gccggggacc cggatcgtga agggcaattg ctggtggatg aagtgctgga ctatctgcaa
   132241 ctggcaccgg aaaagcgcca gcaggtacag cgttgcttga taaacgacct gaacccgcag
   132301 gcggttgagc gggcgatcga ccgtcttcgt tccaacagtg agtttgtacc gctgtgcgtt
   132361 tctgcgctgg cgcgagcgcg tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat
   132421 accattctcg gtcgcaatgc cggttatcag ggcgtacttt ccgtgggacg cgtgcagacg
   132481 cccgtgcttg ggctggtggt gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac
   132541 ttctttgaag tcaaagcaca tatcgtgaca cctgccgatg agcggtttac cgctatctgg
   132601 caaccgagcg aagcgtgtga accgtaccag gatgaagaag ggcgcttgtt acatcgtcca
   132661 ctggcggagc atgtggttaa ccgcattagt ggtcaaccgg ctattgtcac cagctataac
   132721 gataaacggg aatcagaatc cgcgccgctg cctttttcgc tttcagcgtt gcagattgaa
   132781 gcggcaaaac gttttggtct gagtgcgcag aacgtgcttg atatctgcca gaaactgtac
   132841 gaaacgcaca agctaatcac ttatccgcgt tctgattgtc gctatttgcc agaagaacat
   132901 tttgccggac gccacgcggt gatgaatgcc atcagtgttc atgcaccgga tctgttgccg
   132961 cagccagtgg tagatccaga tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg
   133021 caccacgcca tcattccgac cgcacggagt tctgcgatca acctgacgga gaacgaagcg
   133081 aaggtctata acctgattgc ccgtcagtat ctgatgcaat tctgcccgga tgcggtgttc
   133141 cgcaagtgtg ttatcgaact ggacattgcc aaaggcaaat ttgtcgctaa agcgcgtttt
   133201 cttgctgaag caggctggcg cacgctgtta ggcagcaaag agcgcgatga agaaaacgac
   133261 ggcacgctac tgcctgtggt ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg
   133321 gtagagcggc aaacccagcc gccgcgccat tttaccgatg caacactgct ttcggcgatg
   133381 accgggatcg cgcgctttgt gcaggataaa gatctgaaaa agatcctccg tgcgaccgat
   133441 ggtctgggga cagaggcaac gcgtgccggg attattgaac tgttgttcaa gcgtggtttc
   133501 ctgaccaaaa aagggcgcta tatccactcc accgacgccg gaaaagcgct attccattcg
   133561 ctgccggaga tggcgacgcg accggacatg accgcgcact gggaatcggt gctgacgcaa
   133621 atcagcgaaa agcagtgtcg ctatcaggac tttatgcagc cgctggtggg gacgctatat
   133681 cagcttattg atcaagccaa acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg
   133741 ggcagtggtg gcagtgctga taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa
   133801 agtccgccag cagatgaagt cggaagcggg gcgatagcgt aagcgagtga atctttcgtg
   133861 ctattcgagt catattctga aatatccagc ggatcaagaa aattcgttgg atattttttt
   133921 tgcatggata aaattatcgc ctctaaagta tgtaataaca gggaatgtga tgaaaaaagt
   133981 tttattacaa aaccatcctg ggagcgagaa gtattctttt aatggctggg aaatatttaa
   134041 tagtaatttt gaacggatga ttaaagaaaa taaggccatg ctgctttgta agtgggggtt
   134101 ttatttaaca tgtgttgtcg ctgtaatgtt tgtgttcgca gcgataacat ccaacggttt
   134161 gaatgaaaga ggcctgatta ccgcgggatg ctcttttctt tatctattaa ttatgatggg
   134221 gcttattgtt cgggccggtt ttaaagcaaa aaaagaacaa ctgcattatt atcaggctaa
   134281 aggtattgag ccgctcagta tcgagaagtt acaggcgcta caattgatcg caccttatcg
   134341 attctatcat aagcaatggt ctgaaacgct ggagttctgg ccgcgaaagc ctgaacctgg
   134401 caaagatacc ttccaatatc atgtgcttcc ttttgactcg atcgatatca taagtaaaag
   134461 acgcgagtct ttagaggatc aatggggtat cgaagatagc gaaagttatt gtgccttaat
   134521 ggagcatttt ctttctggcg accatggagc caataccttt aaagcaaaca tggaggaagc
   134581 cccagagcag gttatcgcct tgttgaataa atttgctgtt tttccctcag actatatctc
   134641 tgattgcgct aatcatagct ccggtaaatc ctcggcgaag ctaatatggg cggcggaatt
   134701 atcatggatg atctcgatat caagcacagc ttttcaaaac gggacaattg aagaagaact
   134761 ggcctggcat tatataatgc ttgcttctcg aaaggcgcac gagttgttcg aaagcgaaga
   134821 agattatcaa aaaaatagtc aaatgggatt tctttactgg catatctgct gctatcgcag
   134881 aaagttaacg gatgctgaac ttgaagcgtg ttatcgttac gacaagcagt tttgggagca
   134941 ctacagtaag aaatgccgtt ggcccataag aaatgttccg tggggagcat catccgttaa
   135001 atactcataa acgcctgaaa ttttgccggg ggcgctgcgc ttgcccggcc tacattagag
   135061 cgtaactgca atgaattgtg cccatttgta ggcctgataa gcgtagcgcc atcaggcatt
   135121 tacaacttaa atcacaccct gcgccagcat cgcatcggca accttcacaa aaccggcaat
   135181 gttcgcgccc tgcacgtagt tggtttgctc accttcacca ccatgctcaa cacaggcatg
   135241 gtggatatcc agcatgatgt gatgcaaacg tgcgtcaact ttctcggctt tccagcccag
   135301 gcgcgcagcg ttttgtgcca tttccaggcc cgatgtagcg acgccaccag cattagccgc
   135361 tttacccggt gcaaatagta cgcctgcctg ctggaacagt tcagtcgctt cgatggtggt
   135421 cggcatattt gccccttcgg cgacggcttt aacgccatta gcgataagct gatgcgcggc
   135481 gtcaacatcc agttcattct gggtggcgca aggcagggcg atatcaaccg gtagagacca
   135541 cggctgttgg ccttcgagat agaccagacc aaattctttg gcgtaatctg ccactcgacc
   135601 atcgcggctg gctttgattt cgataagacg tgccagtttc tctttcgtga atccgctttc
   135661 atcaactaca gtgccgctgg agtctgacgc agtgatcaca cgagcaccaa attccatcgc
   135721 tttttcgata gcgtactggg cgacgttgcc ggagccagaa acggaaacgc gcatcccttc
   135781 aaaacccata ccgtggcgtt ttagcattgc ttctgtgaaa taaaccagac cgtagccggt
   135841 agcttccggg cgaataagac tgccgccaaa tgaaaggccc ttaccggtga agacgcaggc
   135901 ggtattgttg gagagctttt tcatcatccc cgccataaag ccgacttcac gaccaccaac
   135961 cccgatatca cctgccggaa cgtcggtatc cgcgcccagg tggcgataca gttcagtcat
   136021 cagcgcctgg caaaaacgca tcacttcacc ttcgcttttt cctttcggat cgaaatcgct
   136081 gccgccttta ccaccgccca tcggcagagt agtcagggca tttttgaagg tttgttcaaa
   136141 gccgaggaat ttgagaatgg aaaggttaac tgacggatgg aagcgcatac cgcctttgta
   136201 cgggccgatg gcagagctga actgcacacg ccatgcacgg ttgacctgta tctggttgcg
   136261 atcatcaacc cataccacgc gaaactggat cacgcgctcc ggttcaacca gacgctccag
   136321 taatgacatc tggcgatatt ttggattttg ttcaagaaaa ggccagagtg tggtcattac
   136381 ttcacgaacg gcttgcgcga actcggtttg attcgggtcg cgcttttgga catggttgag
   136441 gaatgactcc agagaatatg tctgatccat agatataaaa cccttatata ttaatacgat
   136501 tgtgcttatg ttgtttatgt catgtgcttt tgcagttttc gactatacca tcaagaatgt
   136561 gacggtaagc aagaaaaaaa tgacccagga aagcaaatta ataacgagag taatctcata
   136621 accaaatgcg ataactcaga cgctaaagtt ttgccacccg gctaccgtta tcagtagcag
   136681 agacaataac gttaaggagt tccctgtgag tcgagcattg ttcgccgtag tattagcatt
   136741 tccattaatt gcactggcta acccgcatta tcgtccggac gtggaggtaa acgtaccgcc
   136801 agaagtgttc agttcaggcg ggcagtcagc gcagccctgt actcagtgct gcgtttatca
   136861 ggatcaaaat tattctgaag gggcggtaat taaagccgaa ggaatactgc tgcaatgcca
   136921 gcgtgatgac aaaaccctta gcactaatcc gctggtctgg cggcgcgtaa agccataaac
   136981 gcctctaata atggaatgtc agcaggggcc agcggatatt gcagcgcctc ttcaggtgag
   137041 caccagacca gcgcctgatg ttcatgtgcc tgtaacgtcc cgtggaagtc gggtacgtgc
   137101 caggcatgaa gatggataat ccgccccgaa acttctcgct gatggctggc aacatattca
   137161 cccacagttg cttcgatgcc cagttcttcg cgtaactcac gcaccagcgc ctgccgctga
   137221 ctttcatccg gctcgacttt accaccggca aactcccata atcccgcctg atcgctctgg
   137281 gcggggcgtt gcgcgagtaa aattttgcca tcacgttcaa tgatggcggc aacaacttca
   137341 atcattttca taaattacac acgggcagat aagcgaaaaa tgccataata tccgcactgt
   137401 tgctcaatca gaaaggcttt atatttaagg agtgcctgtg ctagaccgcc atcttcatcc
   137461 ccggattaaa ccgttgttgc atcagtgcgt gcgggttctt gataaaccgg gcattacgcc
   137521 agatggttta acattagtgg gatttgccat cggcgtgctg gctctgccgt ttctggcgct
   137581 gggctggtat ctggcggcgc tagtcgtcat tttgttgaac aggctgcttg atggtctgga
   137641 cggtgcgctg gcgcggcgca gagagcttac cgacgcgggc ggctttcttg atatttctct
   137701 cgattttctc ttttacgcgc tggtgccgtt tggctttatt cttgctgcac cggagcaaaa
   137761 tgcgctggca ggcggctggt tgttgtttgc gtttatcggc acgggcagca gttttctcgc
   137821 ctttgctgca ctggcagcaa aacatcagat tgataacccc ggttatgcac ataagtcgtt
   137881 ttactatctg gggggattaa ccgaaggcac agaaacgatc ttactgtttg tgctgggatg
   137941 tttattccct gcatggtttg cgtggtttgc gtggatcttc ggtgcgttgt gctggatgac
   138001 gacctttacg cgggtgtgga gtgggtatct gacgctgaag tcgctccagc gtcagtaatt
   138061 gcaatgttat ttgctactgt ccgggccgcg ttcaccggtt gctaccggat tttttggatc
   138121 gctgctccat tcgtaccagc cgccgtcata cacggaaaca ttcttccaac ccatggcgcg
   138181 tgcgtacata aaggtttcgg acgcgcgcca gccggtgccg cagtagaatg aaacttgctg
   138241 ctctggtttg atattccacg ctttccacat agcggtaata tcatcggcgc tgcgcatggt
   138301 gccatccggg ttatgaaaat cttccatatg cgtcgagtcg ctaccagcgt gtccccaacg
   138361 tgcgccggct atttcacctt ttggtttaat atagctgtaa ccgctggtcg taccgataaa
   138421 ttctggccac gaacgaatgc tcaccagcga tgcatcctgg cgatgcagca gtccacgggc
   138481 ctgttccata tcgagcatca actgcggttg tgccgggatc ttcacgccga aatccggttc
   138541 cgctttcact ttcggtggcg ttccgcgctc aacaggcagt cccgcgtcgg accaggtttg
   138601 ccagccgcca tccagcaggc gcacatcttt cacgccagca taaagcataa tctgcgccac
   138661 acgcgctgca gcgtatacgt cacgcccata cagaatgacc gtggtgtcat ggcgaatgcc
   138721 gtgttttgcc agcatcgctt ttagttgttc atcagaaact ttgttccaca gcggttcact
   138781 ttccacttcg ttggtatcga tgtagtcagc gccgggaata tggctgataa ggtaaagctt
   138841 aggagcgccc caggccgctt caatgacttt ccagtcaccg gcaggtttcg ccgtaacctc
   138901 tttaccttgt tgcaggtcgt gcagccattg cggataaacc agctgctcaa aatgcggcag
   138961 tttttgcaga cgggaaggtt cgcttagcgc gtcactcagg atggagatat gcgttaaacc
   139021 tgctttttgc agtcgcgttt tgacggcgtc gacatctttg tcattaccgt acagcgccac
   139081 cggagcatcg gttttcaggt tatgttgctt gatccacgcg ttgagctgtt cggtgctcat
   139141 tttgtcaagc cagctggcag agaggtttaa ggcggcaggt tcatgaccag aagggccatt
   139201 taaggtttgt ggccagccgt tataaaacgc gctggggcga gtatctatcg ctttgccatt
   139261 ttgttgttga agctggtcaa gtgtaagagg cttcgccagt tcagcggccc acgaagaagc
   139321 gcaagccagc cctaaagcca ttgccagcgc ggtcatttga gaaacacgtt tcatcgaaaa
   139381 tcccgacatt gtagttttga gtgcgccatt ctgaactgac gcattgagaa aaactttgcg
   139441 ttatcgcagt ttgttgtaat tttctgacca ctgcgccata tccagaacag aactatcagc
   139501 aggaacatcc tggagatcgt gcgttacctg aacgacgggg atcgccaggg cgcgaacttc
   139561 gctgaacacc cactggcgaa aattatcgcg cagagccaca tcaagacggc tgaatggctc
   139621 atccaggagt aacgcttttg gttgggcgag aagggcgcgt agtagagcaa cgcgcgctcg
   139681 ctgaccgcca gacaaagtgg caggatcctg atggaaagct ccctcaaggc ctgaacgctc
   139741 aagtgcatca ttcacggcat tacgtcgggc attcccttta agtgtcgccg gtagcgccag
   139801 cagtaaattt tgcccgacac tgaactggtc aaataacagt gcatcctgaa aaagaatgcc
   139861 aatctgacgc tgtgcagtgg gtaggatgtc aatccgttgc tcattgagcc atagctcacc
   139921 tgtacaagaa aactgttcgg ccagtgcacc aatcatccat gaaaacagag tggattttcc
   139981 acagccagac ggccccatta acgtgacaat gtcaccttta tccaccgtaa agttaacgtt
   140041 tgtcagcaag cggctttctg gtaaacgtag cgaaacattt ttcacgcaga gcattagcgg
   140101 agtccttgtc tgacataacc tacccatttt gcgactaacg cggtcagggc aaaaataata
   140161 agcggtaata gcagttgcca taaagcctgg gcggcgagaa taccgttgct gccgccgctg
   140221 cttaatgcca ccgcctcagt ggtgagcgtc ggaaaacgcc ccgcgcccag ccacagcgtt
   140281 ggcatatact gcgcaatacc gactgcaaat cccaccgcga aggcaatcag cacagggcgc
   140341 aacatgagtg ggcatttcac gtaaaagaag attttggccc gcgaccagcc cagtgtttgc
   140401 gcaatcaaaa ttaaccgtga atcaatgcgc tgccaggcgg gttgcaggat aaacagcatc
   140461 cacggcatca cccacagcag atgcccccag accaccgccg tccagcttcc atccagtttc
   140521 agccatagcg ccagcgtgta ctggcccgcc accagtggca gagcaggtaa taaaatgggc
   140581 aaccatagcc acaactggcg acgctgtggc ccccattcca gccacagcag taacaggagc
   140641 aaagcgatga atgtcgccac cagccccatt gtcaggctgt tgatgagcgc ttcactattg
   140701 atcgtcgact gatccgcgag gatcgccagc agaaccacac acagcacacc ggttaagggt
   140761 aaaaaaatcg ccagcgtatt gcccggcaat aaaggcgtgg caggcttgcg aacgccatct
   140821 acgcggggaa tagtgcgccg ccagctgcgc catagcagat agctcagcaa aacgtaggcg
   140881 gcgagtaaca gcatcaacag caggctggca agcgcgcctt ttgtttgttg gtcaatgtcg
   140941 ccctgggtta accactgcca gctaattacc gccagcgtcg ggggattacc tggcccgaga
   141001 ataattgcca catccacgac cgacagcgac caggcaacaa tcgccagcat cgccattgcc
   141061 agcgcaggcg cgacggaggg caacagcagc cagttcaggc attgccagcg gctgtagccc
   141121 agcgaatcca gcacaatgac ctgctgcaac agccattttt cgctcaatac tgctgctaag
   141181 atccacagca gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatgccaaat
   141241 cggtccattg gcggagtgaa atacgggaaa tagtcataaa gcagccctcc gtcagcaaag
   141301 agcagaaggg cgctggtggc aaaagccaca tggggaatgg cgagcagcca cggcagacgg
   141361 gcgcacatac gctgccattt cggcccaggc cacagcgcca caatcaccag cagggcaatc
   141421 aacaatgccc cgaccgccgc gatggttgtc gacaccagcg ttgccagtaa tgcctgcggt
   141481 aactgtggat cggcaaataa cgcctgccag tgtgtcaacg acaaagcagg cgatatcagc
   141541 gtgagagctg ccgggatcag cggtgcataa atcaccgcca ccatcgccca cagcaaaaag
   141601 attaatgcgt accgtaacgg tgtagccatt cttgttccag cgcatttacc caacctgcgt
   141661 gcggttcagc cagtaccggc ggcagatcct gcggcattct tgattgcaat gattcgcgct
   141721 gcccgtcagg cagtttttgc ggatcgagaa cagaaggatc gccccagaca gcgggatctg
   141781 ctttacgcag ttgcgcatcg ggtgagagca ggaaattggc aactaccttc gccgcagcac
   141841 tggcattcgc gttggcagga atggtgacga aatgcacgtt gccaatcatc ccctcgcgaa
   141901 agccaaaact gtaactgctt gcaggcaaat cgccgctggc gattttttgc tgcgcatgcg
   141961 cggggttaaa ggtcagcgac aggcgcaatg tgccggcttt cagcagagca tccatccgcg
   142021 cgggtgaagg cgggaaatcc tttccttcgc gccacaaata cggatgcagc acatcaagat
   142081 attgccacaa gggagcagtg acacgggcga aagtcgcatc gtccggcgct tcttttaatg
   142141 cggcgggatc gggcgtcagc ataatcagca actgttcaag aaacgccgtg ccggtaaagt
   142201 ccggtgggcg cggataggta accgtgccgg gattggcttt agcaaactcc agtaaggctt
   142261 gcggcgtttg tggtggctgt ggcgtaacat cgcggcgggc gataaacgtc agttgtgcgc
   142321 cgccccaggg cgattccgca ccttgtgtcg gcacagaaaa atcttcccgc accggcagct
   142381 gtgtgtcgac atagcgccag ttgggcagag tctccgccca gcccgtttgt aataaattgg
   142441 cctcttttaa ggtgcggaag ttttcgccgt tcacccagag cagatccacc gagccgcccg
   142501 ttttacgtcc ggctgcggct tcggtctgaa tgcgcttcac cgcgtctgcg gcatccgcca
   142561 gacggacaat cttcaggttt atagcgtaat gggttttcat ctcgccgctc acccagtcga
   142621 gatagcggtt aattgcggta tcgccgcccc aggcgttaaa ccagacggtt tgccctttgg
   142681 cctcattttt aatttcttgc cagtcactgg catgtgttgc cagagaaaac agcgataaca
   142741 atcccagcaa ccacccacaa tggcgcatca tctctcctta ggaataaaac tagatgtata
   142801 atctattgtt ttttcatttg ttgttttgtt tgtgtttttt aggggccacc aataaggccc
   142861 cttgcgcagt tcgaattctt ctggtgctat ttttcctctt ttttctgtct tcctccatta
   142921 ttctgtttga ctcgatgttc tgtattacga aatcgaatta gagggcagat acttagaaat
   142981 taccagttca gctttccatt agttccggat gtttaaacgt gatatgcgcg gagcccttct
   143041 taaagtatct gatactgaac atctcatctt catagcactc tttaccctga acagactgga
   143101 tgtgccatcc tgatgaatgg ttatgttttt cccgttgccg gggatggggg tgccgctgaa
   143161 cagatgtagc attctttcca ggtcggcgag tcggtcagtc tgttgtccgg tgataagatg
   143221 aaatccccac cggtcccatc tcaccagatt gttgataatg attttacttc caaatttaca
   143281 gggagagttg gttttataat tccagctcag ttccctgaag acgttaatca ctccccgctt
   143341 aaacacctca tccttgttct ggtgcacttg ttcaaatgtg ctcagaatgt tcgcttcgct
   143401 gatttcctga aaattatcgt actccagtga gcggtaccat gtatcacggg cctgtgcatc
   143461 cattaaggaa agcatccctg aacgttgcat caggtcacgc cagatttcac ggtcaaggtt
   143521 gcgggtaatg gctttcattg cggtttcctg tttctccttc agccagcagc cacagcgaaa
   143581 atcctgtttc atggcccagt ccggggcggt tttacctcca atactttccg tcagaattga
   143641 gattttagcc agttgccgga taagtgtgtc gattggcaac agtgtactct ttcgtacagc
   143701 cagaatgtgg cagactgagg tggaacagat aacgtccgta tgcccgctga ccacctccgg
   143761 gcgggagagt gtggtatctg acataatcgt ttttcctttt tgcagataaa tgaaaacgcc
   143821 agctctgttc aggctggcgt caggagaatg aagtctggtg agtgagattc accggttctg
   143881 gtgcagaagt tgcagttggt gcagggtacg gatatcctcc ctggcctgtt cgatacaggg
   143941 caaca
//
LOCUS       DBBGLQ010000002       119473 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.002, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000002 DBBGLQ010000000
VERSION     DBBGLQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 119473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 119473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..119473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.002"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..1276
                     /locus_tag="ACIYSB_000148"
     CDS             <1..1276
                     /locus_tag="ACIYSB_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5427280.1"
                     /translation="YVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNG
                     TELDNSSVDNVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDVNSNATFSAANKAD
                     LGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGL
                     ADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGD
                     MNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNS
                     DAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELG
                     VDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSF
                     TKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            1364..1987
                     /gene="iprA"
                     /locus_tag="ACIYSB_000149"
     CDS             1364..1987
                     /gene="iprA"
                     /locus_tag="ACIYSB_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HGC5427281.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(1988..3145)
                     /gene="ampH"
                     /locus_tag="ACIYSB_000150"
     CDS             complement(1988..3145)
                     /gene="ampH"
                     /locus_tag="ACIYSB_000150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGC5427282.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            3497..4717
                     /gene="sbmA"
                     /locus_tag="ACIYSB_000151"
     CDS             3497..4717
                     /gene="sbmA"
                     /locus_tag="ACIYSB_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGC5427283.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4730..5824
                     /gene="yaiW"
                     /locus_tag="ACIYSB_000152"
     CDS             4730..5824
                     /gene="yaiW"
                     /locus_tag="ACIYSB_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGC5427284.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLINMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAETKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5883..6191)
                     /gene="yaiY"
                     /locus_tag="ACIYSB_000153"
     CDS             complement(5883..6191)
                     /gene="yaiY"
                     /locus_tag="ACIYSB_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGC5427285.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            6451..6663
                     /gene="yaiZ"
                     /locus_tag="ACIYSB_000154"
     CDS             6451..6663
                     /gene="yaiZ"
                     /locus_tag="ACIYSB_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGC5427286.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(6687..7781)
                     /gene="ddlA"
                     /locus_tag="ACIYSB_000155"
     CDS             complement(6687..7781)
                     /gene="ddlA"
                     /locus_tag="ACIYSB_000155"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGC5427287.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            7859..8062
                     /locus_tag="ACIYSB_000156"
     CDS             7859..8062
                     /locus_tag="ACIYSB_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427288.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            8244..8504
                     /gene="iraP"
                     /locus_tag="ACIYSB_000157"
     CDS             8244..8504
                     /gene="iraP"
                     /locus_tag="ACIYSB_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGC5427289.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            8605..10020
                     /gene="phoA"
                     /locus_tag="ACIYSB_000158"
     CDS             8605..10020
                     /gene="phoA"
                     /locus_tag="ACIYSB_000158"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGC5427290.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            10139..10459
                     /gene="psiF"
                     /locus_tag="ACIYSB_000159"
     CDS             10139..10459
                     /gene="psiF"
                     /locus_tag="ACIYSB_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGC5427291.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            10561..11676
                     /gene="adrA"
                     /locus_tag="ACIYSB_000160"
                     /gene_synonym="dgcC"
     CDS             10561..11676
                     /gene="adrA"
                     /locus_tag="ACIYSB_000160"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGC5427292.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(11693..12502)
                     /gene="proC"
                     /locus_tag="ACIYSB_000161"
     CDS             complement(11693..12502)
                     /gene="proC"
                     /locus_tag="ACIYSB_000161"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGC5427293.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            12622..13080
                     /gene="yaiI"
                     /locus_tag="ACIYSB_000162"
     CDS             12622..13080
                     /gene="yaiI"
                     /locus_tag="ACIYSB_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGC5427294.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            13263..13787
                     /gene="aroL"
                     /locus_tag="ACIYSB_000163"
     CDS             13263..13787
                     /gene="aroL"
                     /locus_tag="ACIYSB_000163"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGC5427295.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            13837..14028
                     /gene="yaiA"
                     /locus_tag="ACIYSB_000164"
     CDS             13837..14028
                     /gene="yaiA"
                     /locus_tag="ACIYSB_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGC5427296.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            14286..14963
                     /gene="aroM"
                     /locus_tag="ACIYSB_000165"
     CDS             14286..14963
                     /gene="aroM"
                     /locus_tag="ACIYSB_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGC5427297.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            15035..15319
                     /gene="ppnP"
                     /locus_tag="ACIYSB_000166"
     CDS             15035..15319
                     /gene="ppnP"
                     /locus_tag="ACIYSB_000166"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGC5427298.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            15467..15808
                     /locus_tag="ACIYSB_000167"
                     /pseudo
     CDS             15467..15808
                     /locus_tag="ACIYSB_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            15805..15888
                     /locus_tag="ACIYSB_000168"
     CDS             15805..15888
                     /locus_tag="ACIYSB_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427299.1"
                     /translation="MTQRPWSKLQRKTHNIAALKIIARRSE"
     gene            complement(15966..16877)
                     /gene="rdgC"
                     /locus_tag="ACIYSB_000169"
     CDS             complement(15966..16877)
                     /gene="rdgC"
                     /locus_tag="ACIYSB_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGC5427300.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            17002..17910
                     /gene="mak"
                     /locus_tag="ACIYSB_000170"
     CDS             17002..17910
                     /gene="mak"
                     /locus_tag="ACIYSB_000170"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGC5427301.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(18053..19237)
                     /gene="araJ"
                     /locus_tag="ACIYSB_000171"
     CDS             complement(18053..19237)
                     /gene="araJ"
                     /locus_tag="ACIYSB_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGC5427302.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(19363..22509)
                     /gene="sbcC"
                     /locus_tag="ACIYSB_000172"
     CDS             complement(19363..22509)
                     /gene="sbcC"
                     /locus_tag="ACIYSB_000172"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGC5427303.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNEPAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDAITKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPLDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQLLLTTLTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQD
                     ADNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQ
                     LTRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVS
                     HKTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKV
                     KKINGLGYSKLESTFAVK"
     gene            complement(22506..23708)
                     /gene="sbcD"
                     /locus_tag="ACIYSB_000173"
     CDS             complement(22506..23708)
                     /gene="sbcD"
                     /locus_tag="ACIYSB_000173"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGC5427304.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            23898..24587
                     /gene="phoB"
                     /locus_tag="ACIYSB_000174"
     CDS             23898..24587
                     /gene="phoB"
                     /locus_tag="ACIYSB_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGC5427305.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            24645..25940
                     /gene="phoR"
                     /locus_tag="ACIYSB_000175"
     CDS             24645..25940
                     /gene="phoR"
                     /locus_tag="ACIYSB_000175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGC5427306.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMNEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            26347..27666
                     /gene="brnQ"
                     /locus_tag="ACIYSB_000176"
     CDS             26347..27666
                     /gene="brnQ"
                     /locus_tag="ACIYSB_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGC5427307.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            27742..29115
                     /gene="proY"
                     /locus_tag="ACIYSB_000177"
     CDS             27742..29115
                     /gene="proY"
                     /locus_tag="ACIYSB_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGC5427308.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            29271..31088
                     /gene="malZ"
                     /locus_tag="ACIYSB_000178"
     CDS             29271..31088
                     /gene="malZ"
                     /locus_tag="ACIYSB_000178"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGC5427309.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(31093..31674)
                     /gene="acpH"
                     /locus_tag="ACIYSB_000179"
     CDS             complement(31093..31674)
                     /gene="acpH"
                     /locus_tag="ACIYSB_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGC5427310.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASRKAL"
     gene            31767..32837
                     /gene="queA"
                     /locus_tag="ACIYSB_000180"
     CDS             31767..32837
                     /gene="queA"
                     /locus_tag="ACIYSB_000180"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGC5427311.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSCCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            32893..34020
                     /gene="tgt"
                     /locus_tag="ACIYSB_000181"
     CDS             32893..34020
                     /gene="tgt"
                     /locus_tag="ACIYSB_000181"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGC5427312.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            34043..34375
                     /gene="yajC"
                     /locus_tag="ACIYSB_000182"
     CDS             34043..34375
                     /gene="yajC"
                     /locus_tag="ACIYSB_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGC5427313.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            34403..36250
                     /gene="secD"
                     /locus_tag="ACIYSB_000183"
     CDS             34403..36250
                     /gene="secD"
                     /locus_tag="ACIYSB_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGC5427314.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            36261..37232
                     /gene="secF"
                     /locus_tag="ACIYSB_000184"
     CDS             36261..37232
                     /gene="secF"
                     /locus_tag="ACIYSB_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGC5427315.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            37361..37708
                     /gene="yajD"
                     /locus_tag="ACIYSB_000185"
     CDS             37361..37708
                     /gene="yajD"
                     /locus_tag="ACIYSB_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGC5427316.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(37885..38769)
                     /gene="tsx"
                     /locus_tag="ACIYSB_000186"
     CDS             complement(37885..38769)
                     /gene="tsx"
                     /locus_tag="ACIYSB_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGC5427317.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNYGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(39068..39607)
                     /gene="yajI"
                     /locus_tag="ACIYSB_000187"
     CDS             complement(39068..39607)
                     /gene="yajI"
                     /locus_tag="ACIYSB_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGC5427318.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            39758..40207
                     /gene="nrdR"
                     /locus_tag="ACIYSB_000188"
     CDS             39758..40207
                     /gene="nrdR"
                     /locus_tag="ACIYSB_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGC5427319.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            40211..41314
                     /gene="ribD"
                     /locus_tag="ACIYSB_000189"
     CDS             40211..41314
                     /gene="ribD"
                     /locus_tag="ACIYSB_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGC5427320.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQLLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPVHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            41403..41873
                     /gene="ribE"
                     /locus_tag="ACIYSB_000190"
     CDS             41403..41873
                     /gene="ribE"
                     /locus_tag="ACIYSB_000190"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGC5427321.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            41893..42312
                     /gene="nusB"
                     /locus_tag="ACIYSB_000191"
     CDS             41893..42312
                     /gene="nusB"
                     /locus_tag="ACIYSB_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGC5427322.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            42390..43367
                     /gene="thiL"
                     /locus_tag="ACIYSB_000192"
     CDS             42390..43367
                     /gene="thiL"
                     /locus_tag="ACIYSB_000192"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGC5427323.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            43345..43863
                     /gene="pgpA"
                     /locus_tag="ACIYSB_000193"
     CDS             43345..43863
                     /gene="pgpA"
                     /locus_tag="ACIYSB_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGC5427324.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(43917..44891)
                     /gene="yajO"
                     /locus_tag="ACIYSB_000194"
                     /pseudo
     CDS             complement(43917..44891)
                     /gene="yajO"
                     /locus_tag="ACIYSB_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
     gene            complement(45071..46933)
                     /gene="dxs"
                     /locus_tag="ACIYSB_000195"
     CDS             complement(45071..46933)
                     /gene="dxs"
                     /locus_tag="ACIYSB_000195"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGC5427325.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVILNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSHSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(46958..47857)
                     /gene="ispA"
                     /locus_tag="ACIYSB_000196"
     CDS             complement(46958..47857)
                     /gene="ispA"
                     /locus_tag="ACIYSB_000196"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGC5427326.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(47857..48099)
                     /gene="xseB"
                     /locus_tag="ACIYSB_000197"
     CDS             complement(47857..48099)
                     /gene="xseB"
                     /locus_tag="ACIYSB_000197"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGC5427327.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            48305..49753
                     /gene="thiI"
                     /locus_tag="ACIYSB_000198"
     CDS             48305..49753
                     /gene="thiI"
                     /locus_tag="ACIYSB_000198"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGC5427328.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDNPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(49807..50397)
                     /gene="yajL"
                     /locus_tag="ACIYSB_000199"
     CDS             complement(49807..50397)
                     /gene="yajL"
                     /locus_tag="ACIYSB_000199"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGC5427329.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPVTVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(50360..51271)
                     /gene="panE"
                     /locus_tag="ACIYSB_000200"
     CDS             complement(50360..51271)
                     /gene="panE"
                     /locus_tag="ACIYSB_000200"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGC5427330.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            51439..51930
                     /gene="yajQ"
                     /locus_tag="ACIYSB_000201"
     CDS             51439..51930
                     /gene="yajQ"
                     /locus_tag="ACIYSB_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGC5427331.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(52058..53422)
                     /gene="yajR"
                     /locus_tag="ACIYSB_000202"
     CDS             complement(52058..53422)
                     /gene="yajR"
                     /locus_tag="ACIYSB_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5427332.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLTVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(53571..54461)
                     /gene="cyoE"
                     /locus_tag="ACIYSB_000203"
     CDS             complement(53571..54461)
                     /gene="cyoE"
                     /locus_tag="ACIYSB_000203"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGC5427333.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(54473..54802)
                     /gene="cyoD"
                     /locus_tag="ACIYSB_000204"
     CDS             complement(54473..54802)
                     /gene="cyoD"
                     /locus_tag="ACIYSB_000204"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGC5427334.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(54802..55416)
                     /gene="cyoC"
                     /locus_tag="ACIYSB_000205"
     CDS             complement(54802..55416)
                     /gene="cyoC"
                     /locus_tag="ACIYSB_000205"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGC5427335.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(55406..57397)
                     /gene="cyoB"
                     /locus_tag="ACIYSB_000206"
     CDS             complement(55406..57397)
                     /gene="cyoB"
                     /locus_tag="ACIYSB_000206"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGC5427336.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(57419..58366)
                     /gene="cyoA"
                     /locus_tag="ACIYSB_000207"
     CDS             complement(57419..58366)
                     /gene="cyoA"
                     /locus_tag="ACIYSB_000207"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGC5427337.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(58826..60301)
                     /gene="ampG"
                     /locus_tag="ACIYSB_000208"
     CDS             complement(58826..60301)
                     /gene="ampG"
                     /locus_tag="ACIYSB_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGC5427338.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(60345..60923)
                     /gene="yajG"
                     /locus_tag="ACIYSB_000209"
     CDS             complement(60345..60923)
                     /gene="yajG"
                     /locus_tag="ACIYSB_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGC5427339.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            61228..61545
                     /gene="bolA"
                     /locus_tag="ACIYSB_000210"
     CDS             61228..61545
                     /gene="bolA"
                     /locus_tag="ACIYSB_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGC5427340.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            61889..63187
                     /gene="tig"
                     /locus_tag="ACIYSB_000211"
     CDS             61889..63187
                     /gene="tig"
                     /locus_tag="ACIYSB_000211"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGC5427341.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            63433..64056
                     /gene="clpP"
                     /locus_tag="ACIYSB_000212"
     CDS             63433..64056
                     /gene="clpP"
                     /locus_tag="ACIYSB_000212"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGC5427342.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            64182..65456
                     /gene="clpX"
                     /locus_tag="ACIYSB_000213"
     CDS             64182..65456
                     /gene="clpX"
                     /locus_tag="ACIYSB_000213"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGC5427343.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            65644..67998
                     /gene="lon"
                     /locus_tag="ACIYSB_000214"
     CDS             65644..67998
                     /gene="lon"
                     /locus_tag="ACIYSB_000214"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGC5427344.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            68189..68479
                     /gene="hupB"
                     /locus_tag="ACIYSB_000215"
     CDS             68189..68479
                     /gene="hupB"
                     /locus_tag="ACIYSB_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGC5427345.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            68671..70542
                     /gene="ppiD"
                     /locus_tag="ACIYSB_000216"
     CDS             68671..70542
                     /gene="ppiD"
                     /locus_tag="ACIYSB_000216"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGC5427346.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            70693..71064
                     /gene="ybaV"
                     /locus_tag="ACIYSB_000217"
     CDS             70693..71064
                     /gene="ybaV"
                     /locus_tag="ACIYSB_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGC5427347.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            71158..71556
                     /gene="fadM"
                     /locus_tag="ACIYSB_000218"
     CDS             71158..71556
                     /gene="fadM"
                     /locus_tag="ACIYSB_000218"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGC5427348.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(71608..72303)
                     /gene="queC"
                     /locus_tag="ACIYSB_000219"
     CDS             complement(71608..72303)
                     /gene="queC"
                     /locus_tag="ACIYSB_000219"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGC5427349.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(72368..74068)
                     /gene="ybaE"
                     /locus_tag="ACIYSB_000220"
     CDS             complement(72368..74068)
                     /gene="ybaE"
                     /locus_tag="ACIYSB_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGC5427350.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LQAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQIMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            74168..74986
                     /gene="cof"
                     /locus_tag="ACIYSB_000221"
     CDS             74168..74986
                     /gene="cof"
                     /locus_tag="ACIYSB_000221"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGC5427351.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLVSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            75139..75597
                     /gene="decR"
                     /locus_tag="ACIYSB_000222"
     CDS             75139..75597
                     /gene="decR"
                     /locus_tag="ACIYSB_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGC5427352.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            75627..77399
                     /gene="mdlA"
                     /locus_tag="ACIYSB_000223"
     CDS             75627..77399
                     /gene="mdlA"
                     /locus_tag="ACIYSB_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGC5427353.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFSLLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            77392..79173
                     /gene="mdlB"
                     /locus_tag="ACIYSB_000224"
     CDS             77392..79173
                     /gene="mdlB"
                     /locus_tag="ACIYSB_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGC5427354.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            79354..79692
                     /gene="glnK"
                     /locus_tag="ACIYSB_000225"
     CDS             79354..79692
                     /gene="glnK"
                     /locus_tag="ACIYSB_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGC5427355.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            79722..81008
                     /gene="amtB"
                     /locus_tag="ACIYSB_000226"
     CDS             79722..81008
                     /gene="amtB"
                     /locus_tag="ACIYSB_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGC5427356.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(81057..81917)
                     /gene="tesB"
                     /locus_tag="ACIYSB_000227"
     CDS             complement(81057..81917)
                     /gene="tesB"
                     /locus_tag="ACIYSB_000227"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGC5427357.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            82135..82707
                     /gene="ybaY"
                     /locus_tag="ACIYSB_000228"
     CDS             82135..82707
                     /gene="ybaY"
                     /locus_tag="ACIYSB_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGC5427358.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(82738..83049)
                     /locus_tag="ACIYSB_000229"
     CDS             complement(82738..83049)
                     /locus_tag="ACIYSB_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGC5427359.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            83212..83308
                     /gene="ffs"
                     /locus_tag="ACIYSB_000230"
     ncRNA           83212..83308
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACIYSB_000230"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            83428..83781
                     /gene="ybaA"
                     /locus_tag="ACIYSB_000231"
     CDS             83428..83781
                     /gene="ybaA"
                     /locus_tag="ACIYSB_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGC5427360.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(83823..85373)
                     /gene="pdeB"
                     /locus_tag="ACIYSB_000232"
     CDS             complement(83823..85373)
                     /gene="pdeB"
                     /locus_tag="ACIYSB_000232"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGC5427361.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVAYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESPVLTSEKIPQLLRDMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(85537..86007)
                     /gene="ylaC"
                     /locus_tag="ACIYSB_000233"
     CDS             complement(85537..86007)
                     /gene="ylaC"
                     /locus_tag="ACIYSB_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGC5427362.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(86123..86674)
                     /gene="maa"
                     /locus_tag="ACIYSB_000234"
     CDS             complement(86123..86674)
                     /gene="maa"
                     /locus_tag="ACIYSB_000234"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGC5427363.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(86846..87064)
                     /gene="hha"
                     /locus_tag="ACIYSB_000235"
     CDS             complement(86846..87064)
                     /gene="hha"
                     /locus_tag="ACIYSB_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGC5427364.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(87090..87464)
                     /gene="tomB"
                     /locus_tag="ACIYSB_000236"
     CDS             complement(87090..87464)
                     /gene="tomB"
                     /locus_tag="ACIYSB_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGC5427365.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(88010..91159)
                     /gene="acrB"
                     /locus_tag="ACIYSB_000237"
     CDS             complement(88010..91159)
                     /gene="acrB"
                     /locus_tag="ACIYSB_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGC5427366.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(91182..92375)
                     /gene="acrA"
                     /locus_tag="ACIYSB_000238"
     CDS             complement(91182..92375)
                     /gene="acrA"
                     /locus_tag="ACIYSB_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGC5427367.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            92517..93164
                     /gene="acrR"
                     /locus_tag="ACIYSB_000239"
     CDS             92517..93164
                     /gene="acrR"
                     /locus_tag="ACIYSB_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGC5427368.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            93292..96654
                     /gene="mscK"
                     /locus_tag="ACIYSB_000240"
     CDS             93292..96654
                     /gene="mscK"
                     /locus_tag="ACIYSB_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGC5427369.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWQKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(96866..97027)
                     /gene="rsmS"
                     /locus_tag="ACIYSB_000241"
     CDS             complement(96866..97027)
                     /gene="rsmS"
                     /locus_tag="ACIYSB_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGC5427370.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(97041..97568)
                     /gene="priC"
                     /locus_tag="ACIYSB_000242"
     CDS             complement(97041..97568)
                     /gene="priC"
                     /locus_tag="ACIYSB_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGC5427371.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            97638..98015
                     /gene="ybaN"
                     /locus_tag="ACIYSB_000243"
     CDS             97638..98015
                     /gene="ybaN"
                     /locus_tag="ACIYSB_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGC5427372.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            98168..98719
                     /gene="apt"
                     /locus_tag="ACIYSB_000244"
     CDS             98168..98719
                     /gene="apt"
                     /locus_tag="ACIYSB_000244"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGC5427373.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            98848..100779
                     /gene="dnaX"
                     /locus_tag="ACIYSB_000245"
     CDS             98848..100779
                     /gene="dnaX"
                     /locus_tag="ACIYSB_000245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGC5427374.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    100112..100176
                     /gene="dnaX"
                     /locus_tag="ACIYSB_000245"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            100832..101161
                     /gene="ybaB"
                     /locus_tag="ACIYSB_000246"
     CDS             100832..101161
                     /gene="ybaB"
                     /locus_tag="ACIYSB_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGC5427375.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            101161..101766
                     /gene="recR"
                     /locus_tag="ACIYSB_000247"
     CDS             101161..101766
                     /gene="recR"
                     /locus_tag="ACIYSB_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGC5427376.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            101876..103750
                     /gene="htpG"
                     /locus_tag="ACIYSB_000248"
     CDS             101876..103750
                     /gene="htpG"
                     /locus_tag="ACIYSB_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGC5427377.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSIYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            103931..104575
                     /gene="adk"
                     /locus_tag="ACIYSB_000249"
     CDS             103931..104575
                     /gene="adk"
                     /locus_tag="ACIYSB_000249"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGC5427378.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            104811..105773
                     /gene="hemH"
                     /locus_tag="ACIYSB_000250"
     CDS             104811..105773
                     /gene="hemH"
                     /locus_tag="ACIYSB_000250"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGC5427379.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPEMPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(105770..106729)
                     /gene="aes"
                     /locus_tag="ACIYSB_000251"
     CDS             complement(105770..106729)
                     /gene="aes"
                     /locus_tag="ACIYSB_000251"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGC5427380.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            106881..108185
                     /gene="gsk"
                     /locus_tag="ACIYSB_000252"
     CDS             106881..108185
                     /gene="gsk"
                     /locus_tag="ACIYSB_000252"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGC5427381.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(108318..109994)
                     /gene="ybaL"
                     /locus_tag="ACIYSB_000253"
     CDS             complement(108318..109994)
                     /gene="ybaL"
                     /locus_tag="ACIYSB_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGC5427382.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALLLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(110232..111452)
                     /gene="fsr"
                     /locus_tag="ACIYSB_000254"
     CDS             complement(110232..111452)
                     /gene="fsr"
                     /locus_tag="ACIYSB_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGC5427383.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            111670..113322
                     /gene="ushA"
                     /locus_tag="ACIYSB_000255"
                     /pseudo
     CDS             111670..113322
                     /gene="ushA"
                     /locus_tag="ACIYSB_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
     gene            complement(113359..113838)
                     /gene="ybaK"
                     /locus_tag="ACIYSB_000256"
     CDS             complement(113359..113838)
                     /gene="ybaK"
                     /locus_tag="ACIYSB_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGC5427384.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(114042..114836)
                     /gene="ybaP"
                     /locus_tag="ACIYSB_000257"
     CDS             complement(114042..114836)
                     /gene="ybaP"
                     /locus_tag="ACIYSB_000257"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGC5427385.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            114974..115315
                     /gene="ybaQ"
                     /locus_tag="ACIYSB_000258"
     CDS             114974..115315
                     /gene="ybaQ"
                     /locus_tag="ACIYSB_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGC5427386.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(115530..118034)
                     /gene="copA"
                     /locus_tag="ACIYSB_000259"
     CDS             complement(115530..118034)
                     /gene="copA"
                     /locus_tag="ACIYSB_000259"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGC5427387.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKHLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLHNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            118262..118483
                     /locus_tag="ACIYSB_000260"
     CDS             118262..118483
                     /locus_tag="ACIYSB_000260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427388.1"
                     /translation="MLFARRTSLKATLVNDDGTPRITVEVRNVAPDDIGKVAELVRGV
                     FGTASGACRSDSAVRVDGQDVNAGWLTRL"
     gene            complement(118639..118779)
                     /locus_tag="ACIYSB_000261"
     CDS             complement(118639..118779)
                     /locus_tag="ACIYSB_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGC5427389.1"
                     /translation="MSQKAIIVGILCLTITLVIWMVHDSLCGFSFDIWGARFAAFLQC
                     RP"
BASE COUNT        29111 a        30128 c        31653 g        28581 t
ORIGIN      
        1 ttacgttctg aacgctgatc tggtgaacga ccgtacctgg gatacttcca agtctaacta
       61 cggttacggt attgttgcta tgaactctga tggtcacctg actatcaacg gtaacggcga
      121 cgtagacaac ggtactgaac tggataacag ctctgtagac aatgttgttg ctgcaaccgg
      181 taactacaaa gttcgtatcg acaacgcaac tggcgctggc gctatcgctg attacaaaga
      241 taaagaaatt atctacgtaa acgacgtcaa cagcaacgcg accttctctg ctgctaacaa
      301 agctgacctg ggtgcataca cctatcaggc tgaacagcgc ggtaacaccg ttgttctgca
      361 acagatggag ctgaccgact acgctaacat ggcgctgagc atcccgtctg cgaacaccaa
      421 tatctggaac ctggaacaag acaccgttgg tactcgtctg accaactctc gtcatggcct
      481 ggctgataac ggcggcgcat gggtaagcta cttcggtggt aacttcaacg gcgacaacgg
      541 caccatcaac tatgatcagg atgttaacgg catcatggtc ggtgttgata ccaaaattga
      601 cggtaacaac gctaagtgga tcgtcggtgc ggctgcaggc ttcgctaaag gtgacatgaa
      661 tgaccgttct ggtcaggtgg atcaagacag ccagactgcc tacatctact cttctgctca
      721 cttcgcgaac aacgtctttg ttgatggtag cttgagctac tctcacttca acaacgacct
      781 gtctgcaacc atgagcaacg gtacttacgt tgacggtagc accaactccg acgcttgggg
      841 cttcggtttg aaagccggtt acgacttcaa actgggtgat gctggttacg tgactcctta
      901 cggcagcgtt tctggtctgt tccagtctgg tgatgactac cagctgagca acgacatgaa
      961 agttgacggt cagtcttacg acagcatgcg ttatgaactg ggtgtagatg caggttatac
     1021 cttcacctac agcgaagatc aggctctgac tccgtacttc aaactggctt acgtctacga
     1081 cgactctaac aacgataacg atgtgaacgg cgattccatc gataacggta ctgaagggtc
     1141 tgcggtacgt gttggtctgg gtactcagtt cagcttcacc aagaacttca gcgcctatac
     1201 cgatgctaac tacctcggtg gtggtgacgt agatcaagac tggtccgcga acgtgggtgt
     1261 taaatatacc tggtaatatt cttcactccg aagaaatact ggtaatttaa tctaaataat
     1321 gcccgtcaag gatttgacgg gcattactgc aaaggacgcg caaatgttat ctgtagttaa
     1381 acctcttcag gaatttggta agctcgataa atgtttgtcc agatacggta cgcgcttcga
     1441 gtttaataat gaaaagcaag ttatattttc cagtgatgtc aataacgaag atactttcgt
     1501 tattttagag ggagttatct ctctgcgtag agaagaaaac gtacttatcg gtattaccca
     1561 ggctccttat attatggggc tggctgatgg tttaatgaaa aacgatatac catacaaatt
     1621 aatatcagaa ggaaattgta cgggatatca tctaccagcc aaacaaacca ttacgcttat
     1681 tgaacaaaat caactctggc gagacgcttt ttactggtta gcctggcaaa atagaattct
     1741 ggaattacgc gacgtgcagc tcattgggca taattcctac gaacaaatcc gcgcaacatt
     1801 attatcaatg attgactgga atgaagaatt gcgatcacgt attggtgtga tgaattatat
     1861 ccatcaacgt acacgcatat cgcgttctgt cgtcgcagaa gttctcgctg ctttgcgtaa
     1921 aggcggctat atcgaaatga ataaaggcaa actggtcgct atcaaccgtt tgccttcaga
     1981 gtattaatca ggacgcgggg ataaccaacg gtttattccc gcttagctcg gtcaccaggt
     2041 cattgatgcc atcgctcata tttttaaagc gcgtcaacgg cgaacgagtg accaccacaa
     2101 acgcgccaat attcttctgc gggatcatcg ccatataggt aatgaaaccg ccaccaccgc
     2161 ctgtcttctg aataatcccc ggacgaccct ctttcggggc catatatacc caacctaaac
     2221 caagcgcatc cgctttgccg gggacatcca tgccaatcac tttcgtaaac tgcgcgcgct
     2281 gataaatcaa cgtctgcatg cgatctgcct ggttactgcg ctgataaaaa tcagatgaca
     2341 aatactgctg catccagcgc atcatatcgc caggcgtgga atagacgccg ccgctgccaa
     2401 ttgccgccag cgtgttattg cacggactgg caccgcgctc agcaaccatt aaacggcggc
     2461 actgatccgg tgagggggtg taggtggtgt ctttcatccc caatggacgg gtaatctgct
     2521 cttcaaacaa ctgggtataa ggcttgccgg acgcattcgc cagtgcatct gccagcaggt
     2581 cgaacgcaag gttagagtac gccgcctgac tgcctggcgc ggctttcagc ttcgccgtag
     2641 aaaggtattt ccagcgttgc tcgcgcgttg gccagacaaa caccggacga tgtgccgcgc
     2701 caccgggctg ttcacggggc agggcgctgg tatgggttgc cagattgacc agcgtaatgg
     2761 gcgtcccatt gtaggttggc acgcgtgcgc ctggcggagc atatttactt agcggatcgt
     2821 ttaacttcac ggtcccctgg tcgagcaatt tcaccagcat ctcactggtc attagcttgg
     2881 tgagggaagc aatacgcacg accgaatcca gctgcgggcg aacgttatta ccaggtcgcg
     2941 tttcgccata actgcgaaag acgcgctggt taccgtcgat aacaaccaga gccatccccg
     3001 tggcaccgct gccgtaaaaa atatgatcgg cataacgatc gacaatatca gaggcaaact
     3061 ccggttcagt gatcggctgc gccgcatgga cggaggtcaa tgacgccgca cacagcacgg
     3121 cagaaaaaag cagactacgt ttcaacggtg gtgtccatag attgaatgaa ggtaactgag
     3181 cgtatttata ctactcattg atagattgca aagggcgaat tagtgcaata atagtgttaa
     3241 aaacgtaacg gcgcgtaaac acgatctttt tcctgctcac caatgcctgc atttgtgatc
     3301 ctgcgcgtgt taatccgctt atgtaaacaa tctttggttt gtgaaatccg tcgaaacaat
     3361 tcttatggtc aggctggcga actaagcgcc ttgctatggg tcacaatggg cggtttcatt
     3421 tggctgacgc tttgtagcgg tcatgcggtt aatacacagc agttgttaaa acgataagaa
     3481 gttagcagga gtgcatatgt ttaagtcttt tttcccaaag ccgggaacgt tttttctctc
     3541 ggccttcgtt tgggcattga ttgccgttat cttctggcaa gccggtgggg gtgactgggt
     3601 ggcgcgtatc accggcgctt ccgggcagat cccgattagc gccgcgcgtt tctggtcgtt
     3661 ggatttcctg attttttacg cttactacat tgtttgcgta ggactttttg cattgttctg
     3721 gtttatctac agcccgcatc gttggcaata ctggtcaata ctcggtactg cactgatcat
     3781 cttcgtcacc tggtttttgg tggaagtcgg ggtcgccgtc aacgcctggt atgcgccgtt
     3841 ctatgatctg attcaaaccg cgctaagttc gccgcataaa gtcaccatcg aacaatttta
     3901 ccgcgaagtg ggcgtctttc tggggattgc gctgatcgct gtggtgatca gtgtgctgaa
     3961 caacttcttt gtcagtcact acgtgttccg ctggcgtaca gcgatgaacg aatattacat
     4021 ggcgaactgg caacaactgc gtcatatcga aggggccgca cagcgtgtgc aggaagacac
     4081 catgcgtttt gcttcaacgc tggagaatat gggcgtcagt tttatcaacg ccatcatgac
     4141 gttgatcgcc ttcctgccgg tgctggtaac gctctccgcg catgtgccgg agctgccgat
     4201 tatcgggcac attccgtatg gtctggtgat tgccgcaatc gtctggtcgc tgatggggac
     4261 cggattgctg gcagtggtag ggatcaaact gccggggctg gagtttaaaa accagcgtgt
     4321 agaggctgcc taccgtaaag agctggttta tggtgaagac gatgccacgc gcgcgacgcc
     4381 gcctacggta cgcgagctgt ttagcgccgt acggaaaaac tatttccgcc tctattttca
     4441 ctatatgtat ttcaacatcg cccgcattct ctatttgcag gtcgataacg ttttcggttt
     4501 gttcttgctg tttccgtcaa ttgttgccgg tacgattacg ctcggcctga tgacgcagat
     4561 taccaacgtt tttggtcagg ttcgcggtgc tttccagtac ctgattaact catggaccac
     4621 actggttgag ttgatgtcta tctacaaacg tctgcgcagc tttgaacatg agctggatgg
     4681 tgacaaaatt caggaagtaa cccatacctt gagctaaaaa ggagaagtaa tgtcgcgcgt
     4741 caatcctcta tcttcgctgt cattgttggc ggttctggtt ttagcggggt gtagtagtca
     4801 ggctccgcaa ccgttgaaaa agggcgaaaa agcgatagat gtagcgagcg tcgtgcggca
     4861 gaagatgcct gcgagcgtga aagaccgcga tgcctgggcg aaagatctcg ccaccacctt
     4921 tgaaagtcag gggctggcac cgacgctgga aaacgtctgc tcggtgctgg cggtggcgca
     4981 gcaggagtca aattatcagg cagatccggc cgttccgggc ttaagcaaga tcgcctggca
     5041 agagattgac cgtcgtgctg aacggatgca cattcctgct tttctggttc ataccgcgct
     5101 aaaaatcaaa tcgccaaacg gcaaaagtta tagcgaacgg ttagattcgg tgcgtacaga
     5161 aaagcaattg agcgcgattt ttgacgatct gatcaacatg gtgccaatgg ggcagacgct
     5221 gtttggttcg ctcaatccgg tgcgcaccgg tgggccgatg caggtcagca ttgcttttgc
     5281 cgaacagcat accaaagggt atccgtggaa aatggacggt acagtccgtc aggaagtctt
     5341 cagtcgccgc ggcgggttgt ggtttggtac ttaccattta ctgaattatc ccgccagtta
     5401 tagcgcaccg atataccgtt ttgctgattt taacgctggc tggtacgcca gccgtaatgc
     5461 cgcgtttcag aacgcggtca gtaaggccag cggcgtgaag ctggcactgg acggtgattt
     5521 aattcgttac gacagtaaag aacccgggaa aaccgaactg gcaacgcgca aactggctgc
     5581 aaaactgggg atgagcgaca gcgaaattcg ccgtcagtta gagaaaggtg acagcttctc
     5641 ttttgaggag acggcgctgt acaagaaagt ttatcaactt gccgaaacga aaacgggtaa
     5701 atcactcccc cgcgaaatgt tgcctggcat tcaactggaa agcccgaaaa tcacccgcaa
     5761 cctgactacg gcctggtttg cgaagcgcgt agacgaacgg cgggcgcgtt gtatgaaaca
     5821 gtgatcaaac aggaatgtca ggccagataa ggcgtttcag gccgcatctg acaatgtaaa
     5881 acttactggc ggcgatgtcg ccagtgcagc catagcgcca ccactgcaaa aatgaggcag
     5941 cctactaaaa acgggatcag cccgaaaatg gtgccgacac ctaaaccaat ttccacccgt
     6001 gggcgacctg tttcctggac ctgcattaaa cgctcataga cgccgggcgc atgcactaac
     6061 aggttcagca attgcgcccc agcccaaaag caaaacagca caaacaacgc ataggcaatg
     6121 ttgccaggcg ttgaagaggc attgcgatat tttccgctaa acagcgattt tgacagggtg
     6181 aaatcagcca taaaaacctc cggaagtcat ctgcttctct tctggtgtga taccagcggc
     6241 ttatggcaag tctgacaggt catttagatt gtcaatatca gaatcatggt aaattgatgt
     6301 tgggaatatt cccgaagcgt gaatcttcgt tgtgagtcac aaaatggcaa ccttaagtta
     6361 aatttattaa caattacgaa aatgttgtct acaccacaga cggttgttgc agaatatgca
     6421 aggatgttgt ttttcgttaa cggagctgcc atgaatctgc ctgtaaaaat ccgccgtgac
     6481 tggcactact atgcgttcgc cattggcctt atattcattc ttaatggcgt ggtggggtta
     6541 ctgggatttg aagcaaaagg ttggcagacc tatgccgtcg gtctggtgac gtgggtgatt
     6601 agtttctggc tggcggggtt gattattcgt cgtcgcgatg aagaaactga aaacgcccaa
     6661 taagaatatt atcgggcgtt aaaatattac attgtggttt tcaatgcgtt atccgcagcg
     6721 tgacgctcca gcgccagttc aatcaaacgt gtgatcagat cggtgtaacc cagaccgctg
     6781 gcttgccaca gcttcggata catactgata ttagtgaagc caggcagggt gttgatctcg
     6841 ttgatcacca cttcgttctc tggggttaaa aacacgtcta cacgcgccat gcctgcgcat
     6901 cccaacgttt gataagcctg aacggcaatc gcccggatct tatcgttgat ttctggcgca
     6961 atggctgccg gaactaccac tttcgcgcca tcttcgtcaa tgtacttggt gtcgtaggca
     7021 tagaaatcgc tggtgagtac gatctctcca caggtgctgg cttgcggatt gtcgttgccc
     7081 agaactgcgc attcgatctc acgacctttg atcccttgct caacgatcac tttatgatcg
     7141 aactcgaacg ccagatcgac ggcaattgcg tactgttctt cactggttac tttgctgaca
     7201 ccaacagaag agccctgatt agccggtttt acaaacagtg gtaaccccag tttagactcc
     7261 acttcggcaa aactaatgtt gtgacgattg gcgcgcgtca gggtaataaa tggcgcaatg
     7321 ttcagcccgg catcgcgtag cagacgtttg gtgacatctt tgtccataca ggctgctgaa
     7381 gccagaacat cagaacctac aaacggtaaa ttggcgaccc gcagcattcc ctgcaaggaa
     7441 ccatcttcgc ccagcgtacc gtggacaatc gggaaaatga catccaccgt cggcaacggc
     7501 tgaccgtttt gcgcgtcgat aagctgatgc tcatgtttac ctggcacctg cgcaaggctg
     7561 gtcgccgaag ggcgcaacgc aatatgggca ggatcgtctg catttagcag ataattgctg
     7621 gcatcgctga cgtgccattg cccttgttta tcaatgccca gcagcacaac gtcgaagcga
     7681 cttttatcaa tggcatcgac aatgtttttt gccgattgca gagacacttc atgttccgct
     7741 gatttaccac caaaaacgat tcctacccgc agtttttcca tcttaaaaac ctatcccgtc
     7801 taacacaaag tgcatacatt accacgacaa aacgggggat tcgcggcctt ctgaaagatt
     7861 gttgcaatct tctgctgaca aagcgtgcaa cgtactggtg aagaaagtgc gttatctcaa
     7921 agatgtgcgc aagatcacaa aaatgatgaa cgggaagcta atttattcct ggcttaaatg
     7981 gccatgcggt gagttttttt ctcttaatta taagttaacg aagagaatat atttcataac
     8041 ttttatttat aataaaggtt gataattaaa agcctatatt ttgtgtgggt aattatttaa
     8101 ataagagaaa cgtttcgctg gtaatcaaac aaaaaatatt tgcgcaaagt atttcctttg
     8161 tcataaaaat aatacttcca gacactatga agttgtgaaa cataatgtta acttctccat
     8221 actttggata aggaaataca gacatgaaaa atctcattgc tgagttgtta tttaagcttg
     8281 cccaaaaaga agaagagtcg aaagaactgt gtgcgcaggt agaagctttg gagattatcg
     8341 tcactgcaat gcttcgcaat atggcgcaaa atgaccaaca gcggttgatt gatcaggtag
     8401 agggggcgct gtacgaggta aagcccgatg ccagcattcc tgacgacgat acggagctgc
     8461 tgcgcgatta cgtaaagaag ttattgaagc atcctcgtca gtaaaaagtt aatcttttca
     8521 acagctgtca taaagttgtc acggccgaga cttatagtcg ctttgttttt attttttaat
     8581 gtatttgtac atggagaaaa taaagtgaaa caaagcacta ttgcactggc actcttaccg
     8641 ttactgttta cccctgtgac aaaagcccgg acaccagaaa tgcctgttct ggaaaaccgg
     8701 gctgctcagg gcgatattac tgcacccggc ggtgctcgcc gtttaacggg tgatcagact
     8761 gccgctctgc gtgattctct tagcgataaa cctgcaaaaa atattatttt gctgattggc
     8821 gatgggatgg gggactcgga aattactgcc gcacgtaatt atgccgaagg tgcgggcggc
     8881 ttttttaaag gtatagatgc cttaccgctt accgggcaat acactcacta tgcgctgaat
     8941 aaaaaaaccg gcaaaccgga ctacgtcacc gactcggctg catcagcaac cgcctggtca
     9001 accggtgtca aaacctataa cggcgcgctg ggcgtcgata ttcacgaaaa agatcaccca
     9061 acgattctgg aaatggcaaa agccgcaggt ctggcgaccg gtaacgtttc taccgcagag
     9121 ttgcaggatg ccacgcccgc tgcgctggtg gcacatgtga cctcgcgcaa atgctacggt
     9181 ccgagcgcga ccagtgaaaa atgtccgggt aacgctctgg aaaaaggcgg aaaaggatcg
     9241 attaccgaac agctgcttaa cgctcgtgcc gacgttacgc ttggcggcgg cgcaaaaacc
     9301 tttgctgaaa cggcaaccgc tggtgaatgg cagggaaaaa cgctgcgtga acaggcacag
     9361 gcgcgtggtt atcagttggt gagcgatgct gcctcactga attcggtgac ggaagcgaat
     9421 cagcaaaaac ccctgcttgg cctgtttgct gacggcaata tgccagtgcg ctggctagga
     9481 ccgaaagcaa cgtaccatgg caatatcgat aagcccgcag tcacctgtac gccaaatccg
     9541 caacgtaatg acagtgtacc aaccctggcg cagatgaccg acaaagccat tgaattgttg
     9601 agtaaaaatg agaaaggctt tttcctgcaa gttgaaggtg cgtcaatcga taaacaggat
     9661 catgctgcga atccttgtgg gcaaattggc gagacggtcg atctcgatga agccgtacaa
     9721 cgggcgctgg aattcgctaa aaaggagggt aacacgctgg tcatagtcac cgctgatcac
     9781 gcccacgcca gccagattgt tgcgccggat accaaagctc cgggcctcac ccaggcgcta
     9841 aataccaaag atggcgcagt gatggtgatg agttacggga actccgaaga ggattcacaa
     9901 gaacataccg gcagtcagtt gcgtattgcg gcgtatggcc cgcatgccgc caatgttgtt
     9961 ggactgaccg accagaccga tctcttctac accatgaaag ccgctctggg gctgaaataa
    10021 aaccgcgccc ggcagtgaat tttcgctgcc gggtggtttt tttgctgtta gcaaccagac
    10081 ttaatggcag atcacgggcg catacgctca tggttaaaac atgaagaggg atggtgctat
    10141 gaaaataaca ttactggtta ccttgctttt cggtctggtt tttttaacca ccgtcggcgc
    10201 tgccgagaga actttaaccc cacaacaaca gcgtatgacc tcctgtaatc agcaggcgac
    10261 ggcgcaggcg ttgaaagggg atgctcgtaa gacctacatg agtgattgcc tgaagaacag
    10321 caagtctgcg cctggcgaaa aaagtttgac gccacagcag caaaagatgc gcgaatgcaa
    10381 taatcaagca acacaacaat ctctgaaagg tgatgatcgt aataagttta tgagtgcctg
    10441 cctcaagaaa gccgcctgat acctgatagt gctaacgggt gagctacgaa aatggctcac
    10501 ccgaaatatc atacttctgc ctttagctcc gtctctataa tttgggaaaa ttgtttctga
    10561 atgttcccaa aaataatgaa tgatgaaaac tttttcaaaa aagcggcggc gcacggggag
    10621 gaacctcctt taactcctca aaacgaacat cagcggtccg ggctgcgctt cgcccgtcgc
    10681 gtcagactac cccgtgcggt tggcctggct ggcatgttct taccgattgc ttcaacgctg
    10741 gtttcacacc cgccgccggg ctggtggtgg ctggtgttgg tcggctgggc gttcgtctgg
    10801 ccgcatttag cctggcagat agcgagcagg gccgtcgatc cgcttagccg ggaaatttac
    10861 aacttaaaaa ccgatgcagt attagcggga atgtgggtag gcgtaatggg cgtaaacgtg
    10921 ctgccttcca ccgcgatgtt gatgattatg tgtctgaatt tgatgggggc aggcggcccc
    10981 cgtctgtttg tcgcgggtct ggtgttgatg gtggtttcct gccttgtcac cctcgagctg
    11041 acgggcatta ccgtgtcgtt caatagtgcg ccgctggaat ggtggctctc ccttcccatt
    11101 attgtcattt atcctctgct gtttggctgg gtcagctacc agacggcaac caaactggcg
    11161 gaacataaac gcaggttgca ggtcatgagt acccgcgacg gcatgacggg cgtgtataac
    11221 cgacgtcatt gggaaactat gttacgcaat gaatttgata actgtcggcg gcataatcgc
    11281 gatgcaacgt tactgattat cgatatcgac catttcaaga gcatcaacga tacctggggc
    11341 catgatgtgg gcgatgaagc gattgtggcg cttacccgac agttacaaat taccctgcgc
    11401 ggtagcgatg tgattggtcg gtttggcggc gatgagtttg cagtaatcat gtccggtacg
    11461 ccagctgaga gcgccattac cgccatgtta cgggtgcatg aagggctaaa tacattacgt
    11521 ttgccgaata cgccacaggt aactttacgg attagtgtgg gggttgcgcc gctgaaccca
    11581 caaatgagtc actatcgtga gtggttgaaa tcggcagatt tggcgcttta caaagcaaag
    11641 aaagccggac gtaaccgcac cgaagtggcg gcctgacgtc cggcgaaagt catcaggatt
    11701 tgctgagttt ttctgatttt tccatacact tcgtcatcgc ttcgatcact gcagcacgga
    11761 agcctttctc ttccagtacg cgtaccgctt caatggtggt gcctcccggt gagcagacca
    11821 tatctttcag tgcccccgga tgttctcccg tttccagcac catttttgcg gaacccatta
    11881 ccgcctgagc ggcaaattta tacgcctggg cgcgtggcat cccgcccagc acggcggcgt
    11941 cggccatcgc ttcgataaac ataaatacgt aggctggcga agaaccgctc acaccgacca
    12001 ccgggtggat catcggctca gcaattactt ccgcttcgcc aaagcagcgg aaaatattca
    12061 gcacatcagc ggtatcttct ggggttacca gcgcgtttgg cgttacggag gtcatcccgg
    12121 cattaaccag tgcgggagtg ttcggcatgg cgcggataat tttccggtca tggcccagcg
    12181 cgcgggcaag ctggtcgagc gtgacacctg cagcaataga aacgaccaga gagtctttat
    12241 tcaggctgga ggtgatttcg ctaagcactt taatcatgat gccaggttta acggcagcaa
    12301 aaatgatgtc ggcgatttgc gccacttctt gcgccgattc tgcggcgttg atgccgaact
    12361 ggtcatgcag ggcggcgact ttatccgggg agggggtgta tacccagatt tgccctggaa
    12421 gcacctgacc gctggcaatc agaccgccga gaatggcttt tcccatattg ccgcagccaa
    12481 taaaaccgat tttcttttcc attgcctcac tcctgccgtg aaattcattg ttttgataat
    12541 cgctggcaga agcataaaca gaactatgcc ggaaggcaaa agcgcgacac aatagaggat
    12601 tacccaacaa aggatgactt tatgacaatt tgggtggatg ccgacgcgtg tcccaatgta
    12661 attaaagaga ttttgtatcg cgcggcggaa cgtatgcaga tgccgctggt actggtagca
    12721 aaccagagtt tacgcgtgcc gccatcgcga tttattcgta cgctgcgcgt cgcggcaggt
    12781 ttcgacgttg ccgataacga aattgtccgg cagtgtgaag cgggcgattt ggtgatcacc
    12841 gcagatatac ctttggctgc tgaagccatc gagaaaggcg ctgcggcgct taatccgcgc
    12901 ggcgaacgtt acacgccagc gaccattcgt gagcgcctga cgatgcgcga ttttatggat
    12961 accttacgtg ccagtgggat ccagaccggc ggaccagata gcctttcaca acgtgaccgc
    13021 caggcctttg ccgcggagct ggagaagtgg tggctggaag tgcaacgtag tcgtggctaa
    13081 atgtaattta ttatttacac ttcattcttg aatatttatt gctatagtaa ggggtgtatt
    13141 gagattttca ctttaagtgg aattttttct ttacaatcga aattgtacta gtttgatggt
    13201 atgatcgcta ttctcatgac accggctttc gccgcattgc gacctattgg ggaaaaccca
    13261 cgatgacaca acctcttttt ctgatcgggc ctcggggctg tggtaaaaca acggtcggaa
    13321 tggcccttgc cgattcgctt aaccgtcggt ttgtcgatac cgatcagtgg ttgcaatcac
    13381 agctcaatat gacggtcgcg gagatcgtcg aaagggaaga gtgggcggga tttcgcgcca
    13441 gagaaacggc ggcgctggaa gcggtaactg cgccatccac cgttatcgct acaggcggcg
    13501 gcattattct gacggaattt aatcgtcact tcatgcaaaa taacgggatc gtggtttatt
    13561 tgtgtgcgcc agtatcagtc ctggttaacc gactgcaagc tgcaccggaa gaagatttac
    13621 ggccaacctt aacgggaaaa ccgctgagcg aagaagttca ggaagtgctg gaagaacgcg
    13681 atgcgctata tcgcgaagtt gcgcatatta tcatcgacgc aacaaacgaa cccagccagg
    13741 tgatttctga aattcgcagc gccctggcac agacgatcaa ttgttgattt tcgagcgcct
    13801 atacttaacg ttcatcccgt gaaataagga agaacgatgc caacgaaacc gccttatcct
    13861 cgtgaagcat atatagtgac gattgaaaaa ggaaagccag gacagacggt aacctggtac
    13921 caactcagag ccgatcatcc taaaccagac tcgttgatca gtgaacatcc gaccgctcag
    13981 gaagcgatgg atgcgaaaaa acgctatgag gaccctgaca aagagtgacc gcatcagact
    14041 gctcggaagg gattctgagt gccactacaa gggatctgcg tcacattttt cataattcat
    14101 gtttttctaa taattagaat attaaacaat aacaatccat tactggaatc atttggaatc
    14161 tttacattat gccgtgcacg tctgctgcta cgctttttgt catttgtagc acaagtaagt
    14221 gtcagcagtg gtgcttcaca cttgcccggt aattaacgac gaaagaaaag taaggtggat
    14281 gaacaatgag tgcgtcgttg gcgatcctga ccatcggcat tgtacctatg caggaagttt
    14341 tgccgctcct gacggaatac attgacgaag ataatatttc ccatcatagc ctgctgggga
    14401 agttaagtcg tgaagaagtg atggcggagt acgcgccaga agcaggcgaa gacaccattc
    14461 tcacattatt aaatgacaac cagctggccc atgtttcgcg tcgcaaagtg gagcgtgacc
    14521 tgcaaggtgt ggttgaagtg ctcgataatc agggttatga cgtcatttta ttaatgagta
    14581 cagcaaacat tagtagtatg actgcgcgta atacgatctt tcttgagccg tcgcgaatat
    14641 tgcctccact ggtttcctct attgttgaag atcatcaggt gggggttatc gttccggttg
    14701 aggagatgct gcccgttcag gcgcaaaaat ggcaaatttt gcagaaatcg ccggtatttt
    14761 cattgggtaa ccccattcat gattcagaac aaaaaatcat tgatgccggg aaagaattac
    14821 tggcaaaagg ggctgatgtc atcatgctgg attgtttagg atttcaccaa cgtcatcgcg
    14881 atttactgca aaaacagctc gatgttcctg tcttgctgtc taacgtattg attgcacggc
    14941 tggctgcgga attactggtg taattttgcg tgacagccag cgcctctggc ccctatagtg
    15001 aagtagatgt tcaactacca aacagggcca gtttatgctt caaagtaatg agtacttttc
    15061 cggcaaagtg aaatcaatcg gcttttccag cagcagcact ggtcgcgcca gcgtgggtgt
    15121 tatggttgaa ggcgaataca ccttcagcac cgctgagccg gaagagatga cggtaatcag
    15181 tggcgcgctg aatgtgttac tgcctgacgc gaccgactgg caggtgtatg aagccggttc
    15241 ggtgtttaat gttcccggtc acagtgagtt tcatctgcaa gttgccgaac ccacctctta
    15301 tctgtgccgc tatctgtaat tcctcgcctt ccccttgaac gggagggcat ttttctgaaa
    15361 tatcctttct ttagcccata ataatatttc ctttgctgcg attttttcaa tttccgatat
    15421 attcataatt tatcaaggtt gatataaata tcagtgaaga tctccagata ttgttgcgga
    15481 actggctacg ataaaagata aatcagatga tgaatggtgg cgtgcattgc tgcaaagtcg
    15541 caatgatcat ttacgccaaa cggcattgcg caacgcacat accccggcgt tgttgttaac
    15601 gacattgact gagcctcagg atcggtcgct ggctattaat aatccacagc tggctgccga
    15661 tgtgaaaacg gcgtggttaa aagaggatcc atcattactc ttatttgtcg aacaacccga
    15721 tctttcgctg ttacgtgatt tagtgaaaac cggggcaacg cggaaaattc gcagtgaagc
    15781 gcgtcaccgg cttgaggaaa aacaatgaca caacgtccat ggagcaagtt acagcgtaaa
    15841 acgcataata ttgctgcgct aaaaattatt gctcgccgta gcgaataatt atatgcctgg
    15901 tgtggcttcg tacgccggat aagacgcggc aggcgtcgca tccggcatta aaggaaaatc
    15961 agcaattaac gttgtgcttc gccacctaat ccttcaatca ggttttgaat taacgctgcc
    16021 agttcaccag tcatcaggat gaaatcggca tcaaaacgct gggcgaaatc ttcacggtcg
    16081 atatcttcgt tttgatcgcg cagctcgtcg cagaacttca gacgcttgag cgaaccatcg
    16141 tcgcacatca caaactgaat gcgctgctgc cagtcgagcg ccagtttagt caccactttt
    16201 ccggcttcaa tgtgattggt gatctcttcg ctggtcagat cttgtttctt cgcgcggatc
    16261 acgccgccat cttccagcaa cgatttcagc tcggcttcat caagcagctg gaagccctgt
    16321 gccgcactac cggagcgaac ccattcggtc agcgtcagtt caatcgggtt ttccatgctc
    16381 aatggtacaa ccggtaacga ccccaggctt ttacgcagta atgccagcgt atcttccgct
    16441 tttttggcac tggcgcagtc caccataatc aaaccgttaa ccgtgtcgat ccacatcatt
    16501 gtctggctaa aacggctgaa agcacgcggc agcagagagt gcagcacttc gtctttcagc
    16561 gaatcttttt cggttttctt cagcttacgc gcctgttcag cttccagttt ggcgattttc
    16621 gcttccagcg cctgtttaat caccggagac gggaggattt tttcttcttt gcgcgcgcag
    16681 ataacaattt gaccattggc aacgtgcgtt aacgcatcgc tgtgcgatcc catcggagga
    16741 acccagccca tcttcgccat gtcctggctg ccgcatgggg taaatgccat cgaggctagc
    16801 tgtttttcca tctcttctgc acgcagcgaa atctcgcggc taagacggta aaccattaaa
    16861 tttttgaacc acagcatgat aatttccacg gccttgtcgt taaatttagc gggcatgata
    16921 acgaattgtc ggcggccttg cattgccaat ccggttgtcc gtctctacgc tattgatatt
    16981 gaaaaaaata aggagagtac cgtgcgtata ggtatcgatt taggcggcac caaaactgaa
    17041 gtgattgcac tgggcgatgc aggggagcag ttgtaccgcc atcgtctgcc cacgccgcgt
    17101 gatgattacc ggcagactat tgaaacgatc gccacgttgg ttgatatggc ggagcaggcg
    17161 acggggcagc gcggaacggt aggtatgggc attcctggct caatttcgcc ttacaccggt
    17221 gtggtgaaga atgccaattc aacctggctc aacggtcagc cattcgataa agacttaagc
    17281 gcgaggttgc agcgggaagt gcggctggca aatgacgcta actgtctggc ggtttcagaa
    17341 gcagtagatg gcgcggcagc gggagcgcag acggtatttg ccgtgattat cggcacggga
    17401 tgcggcgcgg gcgtggcatt caatgggcgg gcgcatatcg gcggcaatgg cacggcaggt
    17461 gagtggggac acaatccgct accgtggatg gacgaagacg aactgcgtta tcgcgaggaa
    17521 gtcccttgtt attgcggtaa acaaggttgt attgaaacct ttatttcggg cacgggattc
    17581 gcgatggatt atcgtcgttt gagcggacat gcgctgaaag gcagtgaaat tatccgcctg
    17641 gttgaagaaa gcgatccggt agcggaactg gcattgcgtc gctacgagct gcggctggca
    17701 aaatcgctgg cacatgtcgt gaatattctc gatccggatg tgattgtcct ggggggcggg
    17761 atgagcaatg tagaccgttt atatcaaacg gttgggcagt tgattaaaca atttgtcttc
    17821 ggcggcgaat gtgaaacgcc ggtgcgtaag gcgaagcacg gtgattccag cggcgtacgc
    17881 ggcgctgcgt ggttatggcc acaagagtaa aaaacgtagg caattggcgc atcatgcctg
    17941 atgcgacgct tgccgcgtct tatcaggcct gcaaaatgtg ccagaaccgc gtagggcgga
    18001 taaggcgttc acgccgcatc cggcaataag taatgagcac cgagactata acctacccca
    18061 gtggtttcgc cagcaccgga gtatccgccg cttgctggcg cttatagcga ccatacagca
    18121 gcaacgacga catcgcagca aacgaaagca gggcggcagg cagcgccacg taattatatg
    18181 ccagccccag cgtcagcatc atacctccgc aatatgcgcc gacggcgcta ccgaggttaa
    18241 acgctatttg cccacctgcg gcacctaata actctccgcc tttggcgttt tgtagtaaca
    18301 atatttgtag cggtgctgaa agggcaaata atcccgcgca acaaataaaa gcaaaaataa
    18361 gcgacgttgt tttcatgccg ccgcagaaaa agagcatcag cagtgccagt acaattataa
    18421 agtcagtcac tgctgcaatg cgcagtggtg aataacgtcc tgaaatcctg ccacttagca
    18481 tatttcccag caccatccct agcccaacta acatcataat aaaggtcatc gccgtttccg
    18541 aaaaaccgga aataaacatc atgtatggct ttacgtagct gaaccaggca aacacacctg
    18601 cgttgccaaa catcgtggcg gcgaaaatta accacggggc cgggctgcgc aaaaagtgaa
    18661 attgttcgcg cagatttcct ttcgcctcgt cgcgaatatc tggcacccaa aaatagaccg
    18721 atgccatcac cgcaatatta aaaacagcga tcaataaaaa ggtgtaacgc cagctaaatt
    18781 cctgacttaa atacgttccc agcggaatgc ccagcaaatt ggcgactgtc atcccggaaa
    18841 ccatccccgc cacggcggcg gtgacttttc cgggtttgat aatttttgat aacacgatcg
    18901 ctccgacgcc aaaaaatgcg ccatgcggaa agccggatac cagccgacca atggcgagca
    18961 tcaggtaaga cgaagagagc gtgaacatgg cgttgccaat gacgcacaac gccaccagaa
    19021 acaacaagat atgtttgagt gagtagcggc tggaaaagag tgcgatgatt ggcgcaccga
    19081 ccaccacccc cagtgcataa tacgagatca tatgcccggc ggcaggaatc gaaattccta
    19141 cgttatgcgc cagctccgtg agcacgccca taatgccaaa ttcggccatc cccaaaccaa
    19201 acgtgcccag agccaaagat aaaatgactt ttttcatacc accatcacaa ccaccagaac
    19261 ggctaccaat caccgtagcc aataaagtga tagcttgcag gataacgacc agcaaggcca
    19321 agagataatt cgcccctctg tattcattat cctgctgaat agttatttca ctgcaaacgt
    19381 actttccagt ttgctgtagc ccaggccgtt gatctttttc actttgatct gcaccggaat
    19441 acgctctttc atcgcttcta cgtggctaat cacaccgatg gttttgccac tggcgttcag
    19501 ggcatccagc gcatcaaggg cggtatccag cgtttcgcta tccagcgtgc caaaaccttc
    19561 atcaaggaac agcgagtcaa tacgtgtttt atggctgacc agatccgaaa gcgccagcgc
    19621 cagcgccaga ctaacgagga aactttcgcc gccggaaagg gtacgggtat cgcgtaccgc
    19681 atctgcctgc caggtatcaa caacctcgac ttccagcgcc tcgctggctt tgcgctgtaa
    19741 cagatagcgc ccgtgcagcc gggtaagttg ctgattagcg agatggacta aattatccag
    19801 cgtcagcccc tgggcaaact tgcggaattt atcgccctct ttggaaccta ttagcgaatt
    19861 cagatatccc cagtcctcaa cctgctgcgt catttgagca atttgctgca ttaaggtttg
    19921 ttgttgctga cggttatctg catcctgctt cagctgctgg cgaatctcgc cttgactcgt
    19981 ggtgttttca cgcaactttt ggtgagtttg cgctaactct tgctgaatct gctccaccgt
    20041 cacagtgaga gccaacccgt cgtcaggtcg gtgttgttga tgctgtgcca gcgtttctgc
    20101 tgtctgagtg accagagttt gcgcctgacg gcgctggttt tccagattct gcttgagctg
    20161 ttccagctgc gttagtgttt gttcatccat tagcgccgca aggaacgcct gctgatcgtc
    20221 aaagacgctg gcctgtagcg cggtgtcaaa ctgcgcctgg gctttttgca gactttgcgc
    20281 cgccagaaca tcctgttgct gtaacgtctg ctgctggctg tgtaatgcga gacattgttc
    20341 atgtacctgc cgccagtttt ccaataccac agtttcttcg cagtgcggga gttcatcact
    20401 ttgcggcaac gtttccagaa tcggcgtcag ctgctgaata cggttttgca gcgcggttaa
    20461 ttcgttctgg cgttgctgcc agctctgcgc ttcttgctga cgtgtcgcca accagctctc
    20521 ttcttcatct tcctgtggca atgtcagtgc ataacccgtc aatgtcgtta aaagtagttg
    20581 ctggcgttgt tcaatttgct gttgatactg gataatttgc tgattatgcg cggcaatctg
    20641 cccttgtaat tcatgccgtt ggctgagtaa ccgcagctgg cgttcgtgct catcttgtgc
    20701 atccagccac ggttgaatat cgtccagtgg ctgcaaggtg atattgaggc tggccgtgac
    20761 ggcttgccat tgttgagtaa gtgcttgctc atcttgtcgg aggctttgcg cttcgttttc
    20821 atcacgctga agctgctttg ttatggcgtc cagttgccca cgtagcgtcg caccttcttc
    20881 accgagcttt ttaacttcgt tttccagcgc cagtaatcga gactgattaa cgccaggctc
    20941 cagcgcctga tacgcctcga ccgccgggtg gctggtggaa ccacaaagtg ggcaaggctg
    21001 acccgcctgt aactgtgcac gttgagcttc cagcgttttg atgcgcgctt cctgctcgca
    21061 aatggttttc acatcggcaa gttgctgcgt cttttcttta taacgctggc gcatttcgtt
    21121 aagtgcggcg ttacgttgcg tctgttcttg cgtgacattc tggatagcga cctgtaactg
    21181 cgccagacgt ttttgttggg gaacaatctg tccatgcagc gcgaccaggt gctgacgcag
    21241 tgggcgttgc tcagcatgtt gcgccagggc ggtagcaact tcatcggcgg ttaacgtcaa
    21301 cgtgatcgcc gcaagcgcat taagtttttg ctcagcatgg gttaactgtt gctgccattg
    21361 ccgcagatgc tcgcgatcgc tggtttgttg ggagaactgc gcacgccaac ccgccggttc
    21421 gttgttccac tgacggaagc ggtcgtgttc ctgtaaccag gtattcaggc tttgttgctg
    21481 ctgctgtaat tctgctgact gcttcgccgc gtggtggcga atgctcgcgc gaagcgccat
    21541 tgtgctctgt aagcgagtat ttacttcttc aatctgctgg cgaatatgcg ccagcgccgc
    21601 gctgtgttct gcgatgcgtt cccagtgtgg acgaagattt cgtgccggtt gtgccagact
    21661 aagcgccgcc agttgaggtt gcgctttttc ttcttcggct aacgcctgtt gcaaggcctg
    21721 ctgacggcgg ctggcttctt gctgcaattc gtcctgacgc gttaaccagt ttagcgattg
    21781 ttgttcttgc tgctgcgcgg taattaactg tttttcttcg tcagtaagta cctgcaaact
    21841 cgctgtcagc gattgcactt gttccggcgt gagcaacgtg acgccgctgg cctgcgcttg
    21901 cagcttctcc agctctgtgc gggccgattt gtgctgctca aaaaccatcg ccgagatttg
    21961 cccgtagatt tcagtgccgg ttaactcctc gagcaattcc gcgcgttctt tgggtttggc
    22021 attcaggaag gcagcaaatt gcccctgcga aagcagcatc gaacgggtga agcgcccgta
    22081 atccagcccg gttaacgtcg ctgtcagttc cagcttatct ttcactttgt cggcgagaat
    22141 tttgccgtcg gcgcagcgcg ccagctctac gcgtggcacc tgcaaattac cgtcgggttg
    22201 gttacgcgcc cgattctggc tccagaatgc acggtacgct tcacctttca cttcaaactc
    22261 cacctccgcc agacattcgg cggtatcgcg ggtcatgaga tcattttgcg attgtgaaac
    22321 gttagagaga cgcggagttt cgtgatacag cgccagacaa atggcgtcca gcagggtggt
    22381 tttccccgca cctgttgggc cggtaatagc aaacagcccg ttgctggcga acggctcgcg
    22441 ggtgaaatca atcttccatt cgccttttaa tgagttcagg tttttcaggc gcaggctgag
    22501 aattttcatg cttcgtgttc tccggcgagg gtatgcaacg tcgtggtgaa aagatgctgc
    22561 agacgttgct gctgcgattc atccagttct tccagtgcca gacggcgatt gaacacctct
    22621 tcgacgctga gttcgctgag ggtttcacgc tgttggctgg ctaacacacg ctcgcgctgt
    22681 tcacgactcc gacgtaccag caatacttcg acaggcaatg attcggttaa tgcctggatt
    22741 ttgcgctgaa tatcatgcag atactcatca gtagtgattt cgatatccag ccagacaggt
    22801 ggctcctgcg atacatcgcg ccactgttcc agctgtgcgg taatcgacgc cagatcgcct
    22861 ttcagcactg ccatgggttg cgttaccggt acgttcaggt tttccacgct ctctaatttg
    22921 ccgtttgaaa atgtcaccag atggacatat ttactcttac cgcattcatc aaaactcagt
    22981 ggaatggggg agccgcaata gcgaacatgt tccatgccgc caataatctg tgcgcggtga
    23041 atatgcccga gcgcgatgta gtcggctggt ggaaagtttt gtgccggaaa cgcgtccagc
    23101 gtgccaatat aaatgtcacg cacggcgtca cttttactgg cccccacggt cgttaaatgt
    23161 cccgtggcga tgatgggcag aggctgatcg ccgcgcagtt tgcaggcatc ggcatagtgt
    23221 tgttggtaat aatcggtaat cgctgccagt aaatgctgct gtttttcaat accgttaagc
    23281 cccgcctggc tggtaataat gtcacgcgga cgtaaaaacg gaatggggca cagcactgcg
    23341 cctggcgtcc cgtcgcgacg aggcaagatt tgcggcgcat gtccggcgct ggcgaccacg
    23401 gtagtattga ggaacgccat gatatcgcgc gattcattca gcgtggcgac cgagtcatgg
    23461 tttcctgcca gtaccaccag atgacagcca gtttgctgta aattgacaac aaaacggttg
    23521 tataacgtgc gggcgtaact gggcggcgag ccggtatcga aaacatcacc ggcaacaata
    23581 atcgcatcca cctgatgggt ttgtgctgtc tccagcagcc agtcaagaaa agcctgatgt
    23641 tcagcttcgc ggcttttact gtagaagttc tggccgagat gccagtctga ggtgtgaagg
    23701 atgcgcataa cggttccctg gcgaaaaagc atgggcgcga ttatacccaa acagatgtgc
    23761 catttgcttt tttctgcgcc acggaaatca ataacctgaa gatatgtgcg acgagctttt
    23821 cataaatctg tcataaatct gacgcataat gacgtcgcat taatgatcgc aacctattta
    23881 ttacaacagg gcaaatcatg gcgagacgta ttctggtcgt agaagatgaa gctccaattc
    23941 gcgaaatggt ctgcttcgtg ctcgaacaaa atggctttca gccggtcgaa gcggaagatt
    24001 atgacagtgc tgtgaatcaa ctgaatgaac cctggccgga tttaattctc cttgactgga
    24061 tgttacctgg cggctccggt atccagttca tcaaacacct caagcgcgag tcgatgaccc
    24121 gggatattcc agtggtgatg ttgaccgcca gaggggaaga agaagatcgc gtgcgcggcc
    24181 ttgaaaccgg cgcggatgac tatatcacca agccgttttc gccgaaggag ctggtggcgc
    24241 gaatcaaagc ggtaatgcgc cgtatttcgc caatggcggt ggaagaggtg attgagatgc
    24301 agggattaag tctcgacccg acatctcacc gagtgatggc gggcgaagag ccgctggaga
    24361 tggggccgac agaatttaaa ctgctgcact tttttatgac gcatcctgag cgcgtgtaca
    24421 gccgcgagca gctgttaaac cacgtctggg gaactaacgt gtatgtggaa gaccgcacgg
    24481 tcgatgtcca cattcgtcgc ctgcgtaaag cactggagcc cggcgggcat gaccgcatgg
    24541 tgcagaccgt gcgcggtaca ggatatcgtt tttcaacccg cttttaacgc cttgctcatc
    24601 ggacgcagag cagggcttat gatttcttaa ctggagtatc ttacgtgctg gaacggctgt
    24661 cgtggaaaag gctggtgctg gagctgctac tttgctgcct cccggctttc atcctgggtg
    24721 cattttttgg ttacctgccc tggtttttgc tggcatcggt aacaggactg cttatctggc
    24781 atttctggaa tttattgcgc ctttcatggt ggctgtgggt ggatcgcagt atgaccccgc
    24841 caccggggcg tggtagctgg gaaccgctac tatacggctt acaccagatg cagctgcgaa
    24901 ataaaaaacg ccgccgtgaa ctgggcaatc tgattaaacg ctttcgtagc ggcgcggagt
    24961 cgctgcccga cgcggtggtg ctgaccacgg aagagggcgg tattttctgg tgtaacggtc
    25021 tggcgcaaca aattcttggt ttgcgctggc cggaagataa cgggcagaac atccttaacc
    25081 tactgcgtta cccggagttt acgcaatatc tgaaaacgcg tgatttttct cgcccgctca
    25141 atctggtgct caacaccggg cggcatctgg aaattcgcgt catgccttat acccacaaac
    25201 agttgctgat ggtggcgcgt gatgtcacgc aaatgcatca actggaaggg gcgcggcgta
    25261 acttttttgc caacgtgagc catgagttac gtacgccatt gaccgtgtta cagggttacc
    25321 tggagatgat gaatgagcag ccgctggaag gcgcggtacg cgaaaaagcg ttgcacacca
    25381 tgcgcgagca gacccagcgg atggaaggac tggtgaagca attgctgacg ctgtcgaaaa
    25441 tagaagccgc accgacgcat ttgctcaatg aaaaggttga tgtgccgatg atgctgcgcg
    25501 ttgttgagcg cgaggctcag actctgagtc agaaaaaaca gacatttacc tttgagatag
    25561 ataacggcct caaggtgtct ggcaacgaag atcagctacg cagtgcgatt tcgaacctgg
    25621 tctataacgc cgtgaatcat acgccggaag gcacgcatat caccgtacgc tggcagcgag
    25681 tgccgcacgg tgccgaattt agcgttgaag ataacggacc gggcattgca ccggagcata
    25741 ttccgcgcct gaccgagcgt ttttatcgcg ttgataaagc gcgttcccgg caaaccggcg
    25801 gtagcggatt agggttagcg atcgtgaaac atgctgtgaa tcatcacgaa agtcgcctga
    25861 atattgagag tacagtagga aaaggaacac gtttcagttt tgttatcccg gaacgtttaa
    25921 ttgccaaaaa cagcgattaa tccgcctttg tcatctttta ttgccataag ccagtcgatg
    25981 ctggcttatt ttctttgcag tcaaaatacg ggcgttagat tttacaacgg ttggtgattt
    26041 tttgttcgca tgattagcca tgtctttttc acggaaatag tgttttatac tggttggtga
    26101 tttcttatcg ctatatacct ctggttttta gatccctcct tgctttaaaa cgttataagc
    26161 gtttaaattg cgcttcaggt gctgtcatac tgactgcatt aacgcggtaa atcgaaaaac
    26221 tattcttcgc cgcgcctggt tgggagtatt tcccgctaaa attgtttaaa tataccgctg
    26281 tatcatcccc agggattggc acaaaaattt aacgttacaa caccacatcc acaggcagta
    26341 tgatttatga cccatcaatt aagatcgcgc gatatcatcg ctctgggctt tatgacattt
    26401 gcgttgttcg tcggcgcagg taacattatt ttccctccaa tggtcggctt acaggcaggc
    26461 gaacacgtct ggactgcggc attcggcttc ctcattactg ccgttggcct gccggtatta
    26521 acggtagtgg cgctggcaaa agttggcggc ggtgttgaca gcctcagcac gccaatcggt
    26581 aaagtcgctg gcgtactgct ggcaacggtt tgttacctgg cggtggggcc gcttttcgct
    26641 acgccgcgta cagctaccgt ttcctttgaa gtggggattg cgccgctgac gggtgattcc
    26701 gcgctgccgc tgtttatcta cagcctggtc tatttcgcta tcgttattct ggtttcgctc
    26761 tatccgggca agctgctgga taccgtgggc aacttccttg cgccgctgaa aattatcgcg
    26821 ctggtcatcc tgtctgttgc cgctattgtc tggccggcgg gttctatcag cacggcgact
    26881 gaggcttatc aaaacgctgc gttttctaac ggcttcgtta acggctatct gaccatggat
    26941 acgctgggcg caatggtgtt tggtatcgtt attgttaacg cggcgcgttc tcgtggcgtt
    27001 accgaagcgc gtctgctgac ccgttatacc gtctgggctg gcctgatggc gggtgttggt
    27061 ctgactctgc tgtacctggc gctgttccgt ctggggtcag acagcgcgtc gctggtcgat
    27121 cagtctgcaa acggcgctgc tattctgcat gcttacgttc agcacacctt tggcggcggc
    27181 ggtagcttcc tgctggcggc gttaatcttc atcgcctgcc tggtaacggc agttggcctg
    27241 acctgtgctt gtgcagaatt ctttgcccag tacgtaccgc tctcttatcg tacgctggtg
    27301 tttatcctcg gcggcttctc gatggtggtt tctaacctcg gcttaagcca gctgatccag
    27361 atctccgtac cggtgctgac cgctatttat ccgccgtgta tcgcactggt tgtattaagt
    27421 tttacacgct catggtggca taattcgtcc cgcgtgattg ctccgccgat gtttatcagc
    27481 ctgctttttg gtattctcga cgggatcaaa gcatctgcat tcagcgatat cttaccgtcc
    27541 tgggcgcagc gtttaccgct ggccgaacaa ggtctggcgt ggttaatgcc aacagtggtg
    27601 atggtggttc tggccattat ctgggatcgc gcggcaggtc gtcaggtgac ctccagcgct
    27661 cactaaatca ctgaacattt gttttaacca cggggctgcg atgccccgtg gttttttatt
    27721 gtgttgatgg gttaggaatt gatggaaagt aagaacaagc taaagcgtgg gctaagtacc
    27781 cgccacatac gctttatggc actgggttca gcaattggca ccgggctgtt ttacggttcg
    27841 gcagacgcca tcaaaatggc cggtccgagc gtgttgttgg cctatattat cggtggtatc
    27901 gcggcgtata tcattatgcg tgcgctgggg gaaatgtcgg tacataaccc ggccgccagc
    27961 tctttctcgc gttatgcgca ggaaaacctc ggcccgctgg caggttacat taccggctgg
    28021 acctactgct ttgaaatcct tattgtcgcc atcgccgatg tgaccgcttt tggtatctat
    28081 atgggtgtct ggttcccgac ggtgccgcac tggatttggg tactgagcgt ggtgctgatc
    28141 atttgcgccg taaacctgat gagcgtgaag gtattcggtg agctggaatt ctggttctcg
    28201 ttctttaaag tcgccaccat catcatcatg attgtcgccg gtttcggcat catcatctgg
    28261 gggattggca acggcgggca accgaccggt attcataacc tgtggagcaa cggcggcttc
    28321 ttcagtaacg gctggcttgg catggtaatg tcgttgcaaa tggtgatgtt tgcttacggt
    28381 gggatcgaaa ttatcgggat taccgccggt gaagcgaaag atcctgagaa atcgataccg
    28441 cgtgcgatta actccgtgcc gatgcgtatt ctggtgttct acgtcggtac gctgttcgtc
    28501 attatgtcta tctacccgtg gaatcaggtt ggcactgccg gtagcccgtt cgtgctgacg
    28561 ttccagcata tgggcattac ctttgccgcc agcattctta actttgttgt gctgactgct
    28621 tcgctgtcgg caattaacag tgatgtattt ggcgtaggcc gtatgctcca cggtatggca
    28681 gagcagggca gcgcgccgaa aattttcagc aaaacgtcgc gtcgcggtat tccgtgggtt
    28741 acggtgctgg tgatgactac cgcgctgctg tttgcggtgt atctgaacta catcatgccg
    28801 gaaaacgtct tcctggtgat cgcttcgctg gcaaccttcg ccacggtgtg ggtgtggatt
    28861 atgatcctgc tgtcgcaaat tgccttccgt cgccgtttgc cgccagaaga agttaaggcg
    28921 ctgaaattta aagtgccggg tggggtagca acgaccatcg gcgggctgat tttcctgctc
    28981 tttattatcg ggttgattgg ttatcacccg gatacgcgta tctcgctgta tgtcggtttc
    29041 gcgtggattg ttgtgctgtt gattggctgg atgtttaaac gccgccacga tcgtcagctg
    29101 gctgaaaacc agtaatcctt gcgttctgta agccggttca ggaggaaatc ctgatccggc
    29161 tattccgaaa gttatccgcc cccgtcctcc tcccccaaat atccttcaga tgatgagtga
    29221 tcctgcatta ggctatggca aggtgatcag attttcatca caggggaatt atgatgttaa
    29281 atgcatggca cctgccggtg cccccatttg ttaaacaaag caaagatcaa ctgctcatta
    29341 cactgtggct gacgggcgaa gacccaccgc agcgcattat gctgcgtaca gaacacgata
    29401 acgaagaaat gtcagtaccg atgcataagc agcgcagtca gccgcagcct ggcgtcaccg
    29461 catggcgtgc ggcgattgat ctctccagcg gacaaccccg gcggcgttac agtttcaaac
    29521 tgctgtggca cgatcgccag cgttggttta caccgcaggg cttcagccga atgccgccgg
    29581 cacgactgga gcagtttgcc gtcgatgtac cggatatcgg cccacaatgg gctgcggatc
    29641 agatttttta tcagatcttc cctgatcgtt ttgcgcgtag tcttcctcgt gaagctgaac
    29701 aggatcatgt ctattaccat catgcagccg gacaagagat catcttgcgt gactgggatg
    29761 aaccggtcac ggcgcaggcg ggcggatcaa cgttctatgg cggcgatctg gacgggataa
    29821 gcgaaaaact gccgtatctg aaaaagcttg gcgtgacagc gctgtatctc aatccggtgt
    29881 ttaaagctcc cagcgtacat aaatacgata ccgaggatta tcgccatgtc gatccgcagt
    29941 ttggcggtga tggggcgttg ctgcgtttgc gacacaatac gcagcagctg ggaatgcggc
    30001 tggtgctgga cggcgtgttt aaccacagtg gcgattccca tgcctggttt gacaggcata
    30061 atcgtggcac gggtggtgct tgtcacaacc ccgaatcgcc ctggcgcgac tggtactcgt
    30121 ttagtgatga tggcacggcg ctcgactggc ttggctatgc cagcttgccg aagctggatt
    30181 atcagtcgga aagtctggtg aatgaaattt atcgcgggga agacagtatt gtccgccact
    30241 ggctgaaagc gccgtggaat atggacggct ggcggctgga tgtggtgcat atgctggggg
    30301 aggcgggtgg ggcgcgcaat aatatgcagc acgttgccgg gatcaccgaa gcggcgaaag
    30361 aaacccagcc ggaagcgtat attgtcggcg aacattttgg cgatgcacgg caatggttac
    30421 aggccgatgt ggaagatgcc gccatgaact atcgtggctt cacattcccg ttgtggggat
    30481 ttcttgccaa taccgatatc tcttacgatc cgcagcaaat tgatgcccaa acctgtatgg
    30541 cctggatgga taattaccgc gcagggcttt ctcatcaaca acaattacgt atgtttaatc
    30601 agctcgacag ccacgatact gcgcgattta aaacgctgct cggtcgggat attgcgcgcc
    30661 tgccgctggc ggtggtctgg ctgttcacct ggcctggtgt accgtgcatt tattacggtg
    30721 atgaagtagg actggatggc aaaaacgatc cgttttgccg taaaccgttc ccctggcagg
    30781 tggaaaagca ggatacggcg ttattcgcgc tgtaccagcg aatgattgcg ctgcgaaaga
    30841 aaagtcaggc gctacgtcat ggcggctgtc aggtgctgta tgcggaagat aacgtggtgg
    30901 tatttgtccg cgtgctgaat cagcaacgtg tactggtggc aatcaaccgt ggcgaggcct
    30961 gtgaagtggt gctacccgcg tcaccgtttc tcaatgccgt gcaatggcaa tgcaaagaag
    31021 ggcatgggca actgactgac gggattctgg ctttgcctgc catttcggct acggtatgga
    31081 tgaactaacg ttttataacg ccttgcgtga cgcctgcgcc atcatccgcg gataaaactg
    31141 ccagaagcgg gtttcgaggg cgtcataatg agcgtctaaa tcgtaccagg agtcacgcag
    31201 ggcatccaga cgtgggcggc ggcttgccat gccgtttaac acgttctgga tgaaatccat
    31261 atcgcgatag cgcaccagcc actgctctga ccacaagtaa ttgttcagat tgataaaacg
    31321 tggcggtgag tccggcaaaa tcgtcatcac ttgctcgcgg gcataacaga caaattcctg
    31381 tagcggaaag tccggcgaca gctgcgacca gtggcgggaa agaaagtgat cccacatgac
    31441 atccagcgta ataggcgcaa cgcggcgcgt ttcactacga aaccactccc gtgcttcgcg
    31501 gacttccggc agattgtcag tcaatacgtc gatacgtcga tgcatatgaa tgccagccac
    31561 gacgtcgggc ggaaaacttt cttcaggatt tccgcgtacg aaatcagcca gtaaattgcc
    31621 ggaaagcgag ctttccgcga gatgggctaa atgcaggtga gctaaaaaat tcatcgatta
    31681 tattctatcc aaaagggggt aaaggttgca gggagagcgc cccggcacta gactacccgc
    31741 ctcttatttt agtctgagtc agtgtcatgc gcgttaccga tttctccttt gaattgcccg
    31801 aatccctgat tgcccactat cccatgcctg aacgcagttg ctgtcgttta ctgtcgctgg
    31861 acgggccgac gggcgcgctg acgcacggta ctttcaccga tttacttgat aagctcaacc
    31921 ccggcgatct tctggttttt aataataccc gcgtgatccc ggcgcgcctg tttgggcgta
    31981 aagccagcgg cggcaagatt gaagtgctgg ttgaacggat gctcgacgac aaacgcattc
    32041 ttgcgcatat tcgcgcctcg aaagcgccaa aacctggcgc agaactgctg ctgggcgatg
    32101 acgaaagtat taacgcaaca atgaccgcgc gccacggcgc actgtttgaa gtcgaattta
    32161 atgatgaacg ctcggtgctg gatattctca acagcatcgg ccatatgccg ctgccgccgt
    32221 atatcgaccg tccggacgaa gacgctgacc gcgaacttta tcaaaccgtt tatagcgaaa
    32281 aaccgggcgc ggttgcagcc ccgaccgcag gtctgcattt tgacgagcct ttgctggaaa
    32341 aattgcgcgc caaaggcgtg gagatggcgt ttgtgacgtt gcacgttggt gcgggcacct
    32401 tccagccggt gcgcgtcgac accattgaag atcacatcat gcactcggaa tacgctgaag
    32461 taccgcagga tgtggtagac gcggtactgg cggcgaaagc gcgcggtaac cgggtgattg
    32521 cggttggcac cacttcagta cgttcgctgg aaagcgcggc tcaggcagcg aaaaacgatc
    32581 tcattgaacc gttcttcgac gatacccaaa tctttatcta tccgggcttc cagtacaaag
    32641 tggtcgatgc gctggtgacg aacttccact tgccagagtc gacgctgatt atgctggttt
    32701 cggcctttgc cggttatcaa cacaccatga acgcctataa agcagcggta gaagagaaat
    32761 atcgcttttt tagttacggt gatgcgatgt ttatcacgta caatccgcag gcaattaatg
    32821 agcgcgtcgg ggagtaattc cgcggcgctg gtttaaaacg ttggactgtt tttctgacgt
    32881 agtggagaaa aaatgaaatt tgaactggac accaccgacg gtcgcgcacg ccgtggccgc
    32941 ctggtctttg atcgtggcgt agtggaaacg ccttgtttta tgcctgttgg cacctacggc
    33001 accgtaaaag ggatgacgcc ggaagaagtt gaagccactg gcgcgcaaat tatcctcggc
    33061 aacaccttcc acctgtggct gcgcccgggc caggaaatca tgaaactgca cggcgatctg
    33121 cacgatttta tgcagtggaa ggggccgatc ctcaccgact ccggcggctt ccaggtcttc
    33181 agccttggcg atattcgtaa aatcaccgaa cagggcgtgc acttccgtaa cccgatcaac
    33241 ggcgatccga ttttcctcga tcctgaaaaa tcaatggaga ttcagtacga tcttggttcg
    33301 gatatcgtca tgatctttga tgagtgtacg ccgtatcctg ctgactggga ttacgcaaaa
    33361 cgctccatgg agatgtctct gcgttgggcg aagcgtagcc gtgagcgttt tgacagtctc
    33421 ggaaacaaaa atgcgctgtt tggtatcatc cagggcagcg tttacgaaga tttacgtgat
    33481 atttctgtta aaggtctggt agatatcggt tttgatggct acgctgtcgg cggtctggct
    33541 gtgggtgagc cgaaagcaga tatgcaccgc attctggagc atgtatgccc gcaaattccg
    33601 gcagacaaac cgcgttacct gatgggcgtt ggtaaaccag aagacctggt tgaaggcgta
    33661 cgtcgtggta tcgatatgtt tgactgcgta atgccaaccc gcaacgcccg aaatggtcat
    33721 ttgttcgtga ccgatggcgt ggtgaaaatc cgcaatgcga agtataagag cgatactggc
    33781 ccactcgatc ctgagtgtga ttgctacacc tgtcgcaatt attcacgcgc ttacttgcat
    33841 catcttgacc gttgcaacga aatattaggc gcgcgactca acaccattca taaccttcgt
    33901 tactaccagc gtttgatggc gggtttacgc aaggctattg aagagggtaa attagagagc
    33961 ttcgtaactg atttttacca gcgtcagggg cgagaagtac cacctttgaa cgttgattaa
    34021 tattaataat gagggaaatt taatgagctt ttttatttct gatgcggtag cggcaacggg
    34081 tgcaccggcg caaggtagcc cgatgtcttt gattttgatg ctggtggtat tcggtctgat
    34141 tttctatttc atgatcctgc gtccacagca gaagcgcacc aaagaacaca aaaagctgat
    34201 ggactccatt gccaaaggtg atgaagttct gacgaacggt ggcctggttg gtcgcgtaac
    34261 caaagtagcg gaaaacggct acattgctat cgcgctgaat gacaccactg aagtagttat
    34321 taaacgtgac ttcgtagctg ccgtcctgcc gaaaggcacc atgaaggcgc tgtaattaaa
    34381 atttttccct aagggaattg ccgtgttaaa ccgttatcct ttgtggaagt acgtcatgct
    34441 gatcgtggtg attgtcatcg gtctgctgta tgcgcttccc aacctgtttg gtgaggatcc
    34501 ggctgttcag atcactggtg cgcgcggagt cgccgccagt gagcaaacgc tgatccaggt
    34561 ccagaaaacg ttacaagaag aaaaaataac tgctaagtct gtggcactgg aagagggcgc
    34621 tattcttgcg cgcttcgact ccactgacac ccagttgcgc gctcgtgaag cattaatggg
    34681 cgttatgggt gacaaatacg tcgtggcgct taaccttgcc ccggcaacgc cgcgctggct
    34741 ggcagctatt cacgctgagc cgatgaagct cggccttgac ctgcgtggcg gcgttcactt
    34801 cctgatggaa gtggatatgg acaccgcgct tggcaaactc caggaacaaa atatcgatag
    34861 cctacgcagt gacctgcgcg aaaagggcat cccgtatacc actgttcgta aagaaaacaa
    34921 ctacggcctg agcatcactt tccgcgatgc taaagctcgt gatgaagcca ttgcgtatct
    34981 gagcaagcgc catccggacc tggtgattag cagccagggc agcaaccagc tgcgtgcggt
    35041 aatgagcgat gctcgtctga gtgaagcgcg tgaatatgcg gtgcagcaga acattaatat
    35101 cctgcgtaac cgtgtaaacc aacttggcgt ggcggagccg gtggttcagc gtcagggtgc
    35161 tgaccgtatc gttgttgaac tgccaggtat tcaggacact gcgcgtgcga aagagattct
    35221 gggtgcgacg gcaacgctgg aattccgtct ggtaaacacc aacgttgacc aggccgctgc
    35281 ggcatccggt cgcgtaccgg gcgactctga agtgaaacag acccgcgaag gtcagccagt
    35341 tgtgctgtac aaacgcgtaa ttctgaccgg tgaccatatc accgactcca cttccagcca
    35401 ggacgaatac aaccaaccgc aggttaacat ctcgctcgat agcgctggtg gtaacatcat
    35461 gtctaacttc actaaggaca acatcggcaa accgatggca accctgtttg tggagtacaa
    35521 agacagcggt aagaaagatg caaatggtcg tgcggttctg gtgaaacagg aagaggtgat
    35581 taacatcgcc aacatccagt ctcgtctggg taacagcttc cgtatcaccg gcatcaacaa
    35641 cccgaacgaa gcccgtcagc tgtcactgct gctgcgtgcc ggtgcgttga tcgcgccgat
    35701 tcagattgtt gaagaacgta ccattggccc aaccctgggt atgcagaaca ttgaacaggg
    35761 gctggaggct tgccttgccg gtctgctggt ttctattctg ttcatgatca tcttctataa
    35821 gaagtttggt ctgattgcga ccagtgctct gattgccaac ttgatcttaa tcgtcggcat
    35881 tatgtcgctg ttgccaggcg caacgctgag tatgccaggt atcgcgggta tcgtcttaac
    35941 ccttgcggtg gcggtcgatg cgaacgtact gatcaacgaa cgtattaaag aagagttgag
    36001 caacggacgt actgttcaac aggcaattga tgaaggttat cgtggcgcat tcagttctat
    36061 cttcgatgcg aacatcacca cgctgattaa agtcatcatc ctgtacgcag tgggtaccgg
    36121 ggcaattaaa gggttcgcga ttactaccgg tatcggtgtg gcgacgtcga tgtttaccgc
    36181 gattgtcggt acgcgtgcca tcgtaaacct gctatatggc ggcaagcgcg tcaagaagct
    36241 gtcaatctga ggagtgcgat gtggcacagg aatatactgt tgaacaacta aaccacggcc
    36301 gtaaagtcta tgactttatg cgctgggact actgggcttt cggcatctct ggtctgctgt
    36361 taatcgctgc tatcgttatt atgggcgtgc gcggctttaa ctgggggctg gatttcaccg
    36421 gtggtacggt tattgaaatt acgctcgaaa aaccggctga aattgacgta atgcgtgatg
    36481 cattgcaaaa agccggtttt gaagagccga tgctgcaaaa ctttggtagc agccatgaca
    36541 tcatggtccg tatgccgcct gctgaaggcg aaaccggcgg tcaggtgttg ggcagccagg
    36601 ttctgaaggt gattaacgaa tccaccaatc agaatgcagc agtgaagcgt attgagttcg
    36661 tcggtccgag cgtgggggca gaccttgcgc aaaccggtgc gatggcgttg atggcagcgc
    36721 tgctgtctat cctcgtgtac gtaggtttcc gctttgagtg gcgactggcg gcaggggtgg
    36781 ttattgcgct ggcgcacgac gttatcatta cgctgggtat tttgtcgtta ttccatatcg
    36841 agattgacct gaccattgtg gcatcgttga tgtcggttat cggttactcg cttaacgaca
    36901 gtatcgtggt atcggaccgt attcgtgaaa acttccgcaa gatccgtcgc ggtacgcctt
    36961 acgaaatctt taacgtgtcc ttgacccaga cgctgcaccg taccttgatc acatccggta
    37021 ctaccttgat ggttatcctg atgctgtacc tcttcggtgg tccggtactg gaaggcttct
    37081 cgctgaccat gcttatcggt gtttccatcg gtactgcatc ttccatctat gtggcatctg
    37141 cgttggctct gaaactgggt atgaagcgcg aacacatgtt gcagcagaaa gtggaaaaag
    37201 aaggggcgga tcagccgtca attctgccgt aatcaagttc ccgttgatgt tgaaaatccc
    37261 ggtcagaaga tcgggatttt ttttgatgta tggagattgc gaaaatcccc gcatcttggg
    37321 aaactgcgcg taaccctaca tttcatccag gtaacttttc atggctatca tcccaaaaaa
    37381 ctatgcgcgg ttagaaagcg gctatcgcga aaaagcatta aaaatctatc cgtgggtctg
    37441 cggtcgctgt tcccgcgagt ttgtttattc caacctgcgt gaacttaccg ttcaccacat
    37501 tgatcacgac cataccaata acccggaaga tggcagtaac tgggaattgt tgtgtctcta
    37561 ttgccacgat catgagcatt cgaaatatac cgaagcggat cagtatggta cgaccgttat
    37621 cgcaggggaa gatgcgcaga aagatgtcgg tgaagcgaag tacaacccat tcgctgacct
    37681 gaaagcgatg atgaacaaga agaagtgatt aaaacgtaaa attgcctgat gcgctacgct
    37741 tatcaggcct acgttatttc agcaatatat tgaatttacg tgcttttgtt ggccggacaa
    37801 agcgtttacg ccgcattcgg catgaacaaa gcacacgttg ttaacaatca gaaatgccgg
    37861 gaataaatcc cggcattttc ataatcagaa gttgtaacct actaccaggt aaccacccca
    37921 gccggtagag cgaacgttga agttgccgtt gccgaagttc agttctgcat cgtcgttcca
    37981 ctgaccaccg tcgtgccagt aacgagctac gacagagtag tgccagtgat cgtagttcag
    38041 agccagaata tggctggaag cgatagagtt attagtacgg gtcttaatac cgttgattgc
    38101 gttaccgctg tcatccccta aatcggaacc ccagtcgaag ttggtgaagc cgatgtagct
    38161 cagctgaccg ccccacagat cggtaatcgg cacaaagtat ttaattttga aacggtaacc
    38221 gtcccactcg ttttcgttcg ctgcgccata gttctgccac tggtatttcg catagacgtt
    38281 catggacagg ctcatcggca ggccagtgtc gatatcggta cccagaccca tgtaccaggt
    38341 gctctggcga ccatctttat tacgacccat gtcgtaaatg tagttgttcg cgaagtacca
    38401 ctctttgaac ggaccgaagc taaggtcagt attggtcagc ttgtcgatgg agaaacgtgg
    38461 ttcgatttcc ataaacagcg gagaaccgta gttccagata cctttagcat cggagttacc
    38521 gccgaagaat accggcgcat ccgcataacc atagaagtcg aaccagtctt ttttagcgaa
    38581 tgcttcgtac tcaaggtagg tatcgttgcg gatctgcggt ccgaaacggg tgtgatagct
    38641 tccgacaacg ttaacgctct ggtgccacca gtcggaaaga tactgcggtt tgtcgttttc
    38701 agctgcgttg acagtaaaag acgaagagag cgccagtacc gcaccggctg ccagtaatgt
    38761 ttttttcata tgtatgccac tgtttgaaaa tcccttgcgg gagtgaaaaa ggcgcaaatt
    38821 gcgtttctaa atatttcgta tacagaggag cctattataa aaatcattgc tcacaaaaat
    38881 atgtttcgtt tcacagttct atcatttacg taatcgattg cgttcacgtt tacacacatt
    38941 cgggcgggga ttgtactgac tttcgccctt gttgcaaact tttactatca atccaggtga
    39001 tgtaagcaga gtaacaaaat gacggggaaa gatgttgcgg gacgcacaaa atcgtcccga
    39061 aatacgttta ctgcataaca ggttgaatgt cgtggatacg tacaaagcct aactgatcca
    39121 cagaaatccc ctttaactgt aacggaatat caacatcgct gggggcgagg acgctggcag
    39181 gggcattaat caattgattc tgcacattga tttcctgaaa gttgtctatt gttccctgaa
    39241 tttgcccata ttcaacagtt ccgctgaatg cgggcaaggg atcattcgac tcaccctgaa
    39301 tacgtagtgt aagggtggtg ccatctgcat caggcgtaat attcaccagc gacatacgta
    39361 aagtaccgat ctggctttcc agtcttgccg gtgttttcgc tccaggcaga aggtaaaccc
    39421 cactgctgga ttttgcattc agcctgtttt gctgagtgat tttgactgtt tcttgattga
    39481 gttgcgtcat ctcctggttc agcgtgctga cgctgtgttt catctgtcgc acttcacttt
    39541 gctgcacaca ggcgctaagg ctgaacaggc ttcccagcag gagcagtcga aaaacgttag
    39601 tattcatagg tcgtcccttt ctcgtcgcaa ttagcctaat ggtagagggt aaaagcagcg
    39661 aaagcatcga tcctttatct caaaagcgtt gcgcctttgt tgtatcgtca gttcagggta
    39721 aaatagattt ccgttaacca cctggtcagg acgccgtatg cattgcccat tctgtttcgc
    39781 cgtggacact aaggtaattg actctcgtct cgtgggcgag ggttcatccg tacgccgccg
    39841 tcggcagtgt ctggtgtgta atgaacgttt caccaccttt gaagtggcgg agctggttat
    39901 gccgcgtgtt gtaaaaagca acgacgtgcg tgaaccgttt aatgaagaga aattgcgtag
    39961 cggaatgctg cgggcgctgg aaaaacgtcc ggtgagttcc gatgacgtcg aaatggcaat
    40021 caatcatatt aaatcgcagc tgcgcgccac cggtgagcgc gaagtgccga gcaagatgat
    40081 tggcaatctg gtgatggagc aattgaaaaa gctcgataaa gtcgcctata tccgttttgc
    40141 ctctgtctac cgcagtttcg aagatatcaa agaatttggc gaagagatcg cgcgcctgga
    40201 ggactaagcc gtgcaggacg agtattacat ggcgcgggcg ctaaagctgg cgcaacgagg
    40261 acgttttacc acgcatccca acccgaatgt cgggtgcgtc attgtcaaag atggcgaaat
    40321 tgtcggtgaa ggttaccacc aacgtgcggg tgaaccacat gccgaagtac acgcgttgcg
    40381 tatggcgggt gaaaaagcca aaggtgcgac cgcctatgtc acactcgaac cctgtagcca
    40441 tcatggtcgt acgccaccgt gctgtgacgc actcatcgcc gctggcgtag cgcgcgtggt
    40501 tgcctcgatg caagatccta acccgcaggt cgctgggcgt ggactttacc gtctgcaaca
    40561 ggctggcatt gacgtcagcc acggcctgat gatgagtgaa gccgagcaat tgaataaagg
    40621 ctttctcaag cggatgcgca ccggctttcc ttatattcag ttaaaacttg gcgcatcgct
    40681 tgatggtcgc acggcgatgg cgagcggcga aagccagtgg atcacttcgc cccaggcgcg
    40741 gcgtgatgta caactactgc gcgcgcaaag tcatgccatt ttaaccagca gcgccacggt
    40801 gctggcggat gatcctgcct taacggtgcg ttggtctgaa ctggatgaac aaactcaggc
    40861 gctctatccg caacaaaatc tccgtcagcc gatacgtatt gtgattgata gccaaaatcg
    40921 cgtgacgccg gtacatcgca ttgtgcagca gcccggcgaa acctggttcg cgcgtacgca
    40981 ggaagattct cgtgagtggc cggaaacggt gcgtaccttg ctgattccag agcataaagg
    41041 tcatctggat ctggttgtac tgatgatgca actgggtaaa cagcaaatta acagcatctg
    41101 ggtggaagcg gggccaacgc tcgctggcgc attgctgcag gcgggtttag tcgatgagct
    41161 gattgtctat atcgcaccta aactattagg cagcgacgcc cgcggattat gcacgctgcc
    41221 agggcttgag aaattagccg acgcccccca atttaaattc aaagagatac gtcatgtagg
    41281 cccggatgtt tgcctgcatt tagtgggtgc atgatctccc ggctcgaaag ggaagcagcg
    41341 cacgaaatat tatgctaaaa tccgcccccc tgcggggcca tactcgaacc cgaaggaaga
    41401 aaatgaacat tattgaagct aacgttgcta ccccggacgc tcgcgtcgcc atcaccatcg
    41461 cgcgtttcaa caactttatc aatgacagcc tgctggaagg tgcaattgac gcactgaaac
    41521 gtatcggtca ggtaaaagat gaaaacatta ccgttgtttg ggtgcctggt gcctatgagc
    41581 tgccgctggc ggcgggtgca ctggctaaaa ccggtaaata cgacgcggtg attgcgctgg
    41641 gtacggttat tcgtggtggc actgcccact ttgaatatgt cgctggtggt gcaagcaacg
    41701 gcctggcgca tgttgcccag gacagcgaaa ttccggttgc ttttggggtt ctgaccactg
    41761 aaagcattga acaagcgatc gaacgtgctg gcaccaaagc tggcaacaaa ggtgcagaag
    41821 ctgcactgac cgcgcttgaa atgattaatg tattgaaagc catcaaggcc tgaaattagt
    41881 aaggggaaat ccgtgaaacc tgctgctcgt cgccgcgctc gtgagtgtgc cgtccaggcg
    41941 ctctactcct ggcagttgtc ccagaacgac atcgctgatg ttgaatacca gttcctggct
    42001 gaacaggatg taaaagacgt tgacgtcctg tacttccgtg agctgctggc cggggtggcg
    42061 actaataccg catacctcga cggactgatg aagccatacc tgtcccgcct gctggaagaa
    42121 ctgggacagg tagaaaaagc agtactgcgc attgcgctgt acgaactgtc taaacgtagc
    42181 gatgtgccat acaaagtggc cattaacgaa gcgatcgaac tggcgaaatc gttcggcgca
    42241 gaagacagcc ataagttcgt caacggcgta ctcgataaag cagcacctgt gattcgccct
    42301 aacaaaaagt gatatccagg ccggtagatt cacggaagac cgttccatga tcgccggcct
    42361 tttctttttt acctgctgag gcataacgta tggcatgtgg cgagttctcc ctgattgccc
    42421 gttattttga ccgtgtaaga agttctcgtc ttgatgtcga actgggcatc ggcgacgatt
    42481 gcgcacttct caatatcccc gagaaacaga ccctggcgat cagcactgat acgctggtgg
    42541 cgggtaacca tttcctccct gatatcgatc ctgctgatct ggcttataaa gcactggcgg
    42601 tgaacctaag cgatctggca gcgatggggg ccgatccggc ctggctgacg ctggcattaa
    42661 ccttaccgga cgtagacgaa gcgtggcttg agtccttcag cgacagtttg tttgatcttc
    42721 tcaattatta cgatatgcaa ctcattggcg gcgataccac gcgtgggcca ttatcaatga
    42781 cgttgggtat ccacggcttt gttccgatgg gacgagcctt aacgcgctct ggggcgaaac
    42841 cgggtgactg gatctatgtg accggtacac cgggcgatag cgccgccggg ctggcgattt
    42901 tgcaaaaccg tttgcaggtt gccgatgcta aagatgcgga ctacttgatc aaacgtcatc
    42961 tccgtccatc gccgcgtatt ttacaggggc aggcactgcg cgatctggca aattcagcca
    43021 tcgatctctc tgacggtttg atttccgatc tcgggcatat cgtgaaagcc agcgactgcg
    43081 gcgcacgtat tgacctggca ttgctgccgt tttctgatgc gctttctcgc catgttgaac
    43141 cggaacaggc gctgcgctgg gcgctctctg gcggtgaaga ttacgagttg tgtttcactg
    43201 tgccggaact gaaccgtggc gcgctggatg tggctctcgg acacctgggc gtaccgttta
    43261 cctgtatcgg gcaaatgacc gccgatatcg aagggctttg ttttattcgt gacggcgaac
    43321 ctgttacatt agactggaaa ggatatgacc attttgccac gccataaaga tgtcgcgaaa
    43381 agtcgcctga agatgagtaa tccgtggcat ctacttgctg tcggattcgg aagtggatta
    43441 agcccgatcg ttcctgggac gatgggctcg ctggcagcga ttccgttctg gtatctgatg
    43501 acctttttgc cctggcagct ctactcgctg gtggtgatgc tggggatctg tatcggcgtc
    43561 tatctttgtc atcaaacggc gaaagacatg ggtgtgcacg atcatggcag cattgtctgg
    43621 gacgaattta ttggtatgtg gatcacgctc atggcgctgc cgaccaatga ctggcagtgg
    43681 gttgccgccg ggtttgtgat tttccgtatt ctggatatgt ggaagccgtg gccgatccgc
    43741 tggtttgatc gcaatgtgca tggcggcatg gggatcatga tcgacgatat tgtcgccggg
    43801 gtgatttccg caggcatcct gtattttatc ggtcatcact ggccgctggg tattctgtcg
    43861 tagttgtgca ccgatgcctg atgtgacgct tgtcacgtct catcaggcct ggactcttat
    43921 ttaaatccta cgacaggatg cggtttatac ggcgtttcca gttcggcaat ctgttccggc
    43981 ttcaaagtga tatccaccgc gttcaatagc tcatcaagct gttcttcgcg cgaagttccg
    44041 ataatcggtg cggcaatgcc cggtttactc aacaaccagg ccagcgcaac ttgtgctcgt
    44101 gtcgccccca gttcttcact gacgcctgtt aaccgctctg cgatctgcgc gtcattttca
    44161 tcgctttctt tatagagatt tttccccacc tcatcagaca ccagtcgtgc ggtagtttct
    44221 ccccacggac gcgtcagacg gccccttgcc agcgggctcc atggaattac cgccacgccc
    44281 tcctgataac acagtggtag catctcgcgc tcttcttcac gataaatcag attgtagtga
    44341 tcctgcatac tgacaaactg cgcccagccg tgctgttttt ggagttccag tgcctgagca
    44401 aactgcgaag cgtgcattga tgacgcgccg atctaacgcg ctttcccggc ttttaccacg
    44461 tcgttgaggg cttccagcgt ctcttcgatc ggcgtgttgt aatcccagcg atgaatttgc
    44521 aggatatcga cataatccat gccgagacgt cgcaggctgt cgtcgataga gcgcaaaatt
    44581 tgcgcacggg ataatccttc cggtaaatca ccaacgcgat ggaacacttt ggtcgcaacg
    44641 accacgtctt cacgacgggc gaaatcccgc agtgcgcgac cgacgatctc ttcgctgctg
    44701 ccgtcagaat aactgttggc ggtatcaaag aaatttatgc cgccttccag tgcacgttta
    44761 attatgggac ggctgctttc ttccggcagt gtccatgcgt gattaccgcg atctggctcg
    44821 ccaaaggtca tacagccgag gcaaagtcgg gaaacgcgaa gatcggtttt tcctaagggg
    44881 ttgtattgca tgctgccact cctgctatac tcgtcatact tcaagttgca tgtgctgcgg
    44941 ctgcattcgt tcaccccagt cacttactta tgtaagctcc tggggcttca ctcgtttgcc
    45001 gccttcctgc aactcgaatt atttagagtc tatgaataat ttcttaagca tagcaggagt
    45061 ggagtaggga ttatgccagc caggccttga ttttggcttc cataccagcg gcatcgaggc
    45121 cgagttcggc gcgcatttct tcctgagttc cttgcggaat aaagaagtcc ggcaggccaa
    45181 tgttcagcac gggtactggt ttacgatggg ccatcagcac ttcgttcacg ccgctgcctg
    45241 cgccgcccat aatggcgttt tcttctacgg tgaccagcgc ttcatggctg gcggccattt
    45301 ccagaattaa cgcttcatca agcggtttca caaaacgcat atcgaccagc gtggcgttca
    45361 gcgattcggc gactttcgcc gcttctggca tcagcgtacc aaagttaagg atcgccagtt
    45421 tctcgccacg acgcttcaca atgcctttgc caattggtag tttttccagc ggcgtcagtt
    45481 ccacgccgac cgcgttgcca cgcgggtagc gcaccgctga cgggccatcg ttatagtgat
    45541 agccggtata gagcatctgg cgacattcgt tttcatcgct cggggtcata atgaccattt
    45601 ccggtatgca gcgcaggtaa gagagatcaa aagcaccctg atgggtttga ccgtcagcac
    45661 caacaatgcc cgcgcggtcg atggcgaaca ggaccggaag cttttgaatc gccacgtcat
    45721 gcagcacctg atcataggcg cgttgcagga aagtggagta aatcgcgaca atgggtttgt
    45781 acccaccaat cgccagaccc gcagcaaagg tcaccgcgtg ttgctcggca attgccacgt
    45841 cgaagtagcg atccgggaat ttacgtgaaa actcgaccat gccggaacct tcacgcatcg
    45901 ccggagtaat cgccatcagc ttgttgtctt tcgctgccgt ttcgcacaac cagtcgccaa
    45961 agatttttga atggctcggc aaaccgccgc tacttttcgg caaacaaccg ctggagggat
    46021 caaatttagg cacggcgtgg aaagtgatcg ggtctttttc tgccggttca taaccacgac
    46081 cttttttggt catgatatgc aggaactgcg ggcctttcag gtcgcgcatg ttctttagcg
    46141 tggtgataag ccccagcaca tcgtgaccgt ccaccgggcc gatgtagtta aagcccagct
    46201 cttcaaacaa cgtgccaggc actaccatgc ctttaatatg ttcttcggtg cgtttgagca
    46261 gctctttaat tggcggcacg ccagagaaaa cttttttccc gccttcgcgc agtgaagagt
    46321 aaagcttacc ggaaagcagc tgtgccagat ggttgttgag cgcgccgaca ttttcggaaa
    46381 tcgacatttc attgtcgttg agaatcacca gcatatcagg acggatatcg cccgcgtgat
    46441 tcatcgcttc aaacgccatg cctgcggtaa tcgcgccatc gccaatgaca cagacggtgc
    46501 ggcgattttt gccttctttt tcggcagcaa ccgcaatacc aattccggca ctgatggagg
    46561 ttgatgaatg cccgacgctt aatacgtcat attcgctttc gccgcgccac gggaacgggt
    46621 gcagaccgcc tttctgacgg atggtgccga ttttgtcgcg gcgtccggtc aaaattttat
    46681 gcggataagc ctgatgcccc acatcccaaa tcaattggtc aaacggggtg ttgtagacat
    46741 agtgcagcgc cacggtcagt tcgaccgtgc ccagcccgga ggcgaagtgc ccgctggaac
    46801 ggctcacgct gtcgagtaaa tagcggcgca gttcgtcgca gagtttcggt aaactctctt
    46861 tcggcaacag tcgtaactcc tgggtggagt cgaccagtgc cagggtcggg tatttggcaa
    46921 tatcaaaact catcaggggc ctattaatac ttattgttta tttattacgc tggatgatgt
    46981 agtccgctag cgcttccagt gccgaggtat cgagtgactg ttcagccagt tgtttcagcg
    47041 actgacgggc atcgtcgatc agatcccggg ctttcttccg ggcttgctca agacccagaa
    47101 gtgcagggta ggtactttta ccaagttgct ggtcggcacc ctggcgtttt cccaacgttg
    47161 cagtatctcc caccacatcc aggatgtcat cctgaacctg gaaggcaagg ccgatgctct
    47221 ctgcatactt gtcgagtacc ggcagagcac gacgtccttt atctccggcg cttaatgcac
    47281 caaggcgaac ggcggcgcga atcaatgcgc cggttttatg acgatgaata cgctcaagcg
    47341 cgtccagagg tacgtgtttg ccttccgcgt ctaaatctaa tgcctgacca ccgcacattc
    47401 cggcaatacc actggcgctc gccagttcag aaatcatcga aattctgtcg cggtccgaca
    47461 cttccggcat atcggcatcg cttaaaatcg agaacgccag cgtttgtaaa gcgtcgccag
    47521 cgagaatcgc gtttgcttcg ccaaacttca catggcaggt tggcaaaccg cgacgcagat
    47581 cgtcatcatc cattgccggt aaatcatcat gaattaatga gtaagcgtgg atacactcaa
    47641 cggcggcagc gggtgcgtcc agcgtgtttg tgctaacgcc gaacatatga ccggtggcat
    47701 aaaccaggaa aggtcgcagg cgcttaccac ctaataatgc gccatactgc atggtttcga
    47761 ccacgggagt gttctgaaag ggcagtgggg cgataaaacg gctcagcgcc tggttggcct
    47821 gcttaacgca ggcttcgagt tgctgcggaa agtccattac tcattgtccg gtgtaaaagg
    47881 ggttagagag gcgtcttcat tgtcagacag cagaatttgt acgcgctgtt cggcttgttg
    47941 taatttggcc tgcccctgac gtgccagctg cacgccgcgt tcgaactcgt tcagcgcctc
    48001 ttccagcggc aggtcgccac tttccagacg ggttacaatc tgttccagct cgctcagcgc
    48061 cttttcaaag ctggcgggcg cctcattttt cttcggcata atgaatgtct gactctcaat
    48121 atttttcgcc ccgtcatggt aacggactca gggcaaatag caaataacgc gcaatggtaa
    48181 ggtgatgtgc acagcaaagc gatgttagtg gtatacttcc gcgcctggat gcagccgcag
    48241 gtgtgggctg ctgtattttt ccctatacaa gtcgcttaag gcttgccaac gaaccattgc
    48301 cgccatgaag tttatcatta aattgttccc ggaaatcacc atcaaaagcc aatctgtgcg
    48361 cttgcgcttt ataaaaatcc ttaccgggaa cattcgtaac gttttaaagc actatgatga
    48421 gacgctcgct gtcgtccgcc actgggataa catcgaagtt cgcgcaaaag atgaaaacca
    48481 gcgtctggct attcgcgacg ctctgacccg tattccgggt atccaccata ttctcgaagt
    48541 cgaagacgtg ccgtttaccg acatgcacga tattttcgag aaagcgttgg ttcagtatcg
    48601 cgatcagctg gaaggcaaaa ccttctgcgt acgcgtgaag cgccgtggca aacatgattt
    48661 tagctcgatt gatgtggaac gttacgtcgg cggcggttta aatcagcata ttgaatccgc
    48721 gcgcgtgaag ctgaccaatc cggatgtgac tgtccatctg gaagtggaag acgatcgtct
    48781 cctgctgatt aaaggccgct acgaaggtat tggcggtttc ccgatcggca cccaggaaga
    48841 tgtgctgtcg ctcatttccg gtggtttcga ctccggtgtt tccagttata tgttgatgcg
    48901 tcgcggctgc cgcgtgcatt actgcttctt taacctcggc ggcgcggcgc atgaaattgg
    48961 cgtgcgtcag gtggcgcatt atctgtggaa ccgctttggc agctcccacc gcgtgcgttt
    49021 tgtcgctatt aatttcgaac cggtcgtcgg ggaaattctc gagaaaatcg acgacggtca
    49081 gatgggcgtt atcctcaaac gtatgatggt gcgtgccgca tctaaagtgg ctgaacgtta
    49141 cggcgtacag gcgctggtca ccggcgaagc gctcggccag gtgtccagcc agacgctgac
    49201 caacctgcgc ctgattgata acgtctccga cacgctgatc ctgcgtccgc tgatctctta
    49261 cgacaaagag cacatcatca acctggcccg ccagattggc accgaagact ttgctcgcac
    49321 gatgccggaa tattgtggtg tgatctccaa aagcccgacg gtgaaagcag ttaaatcgaa
    49381 gattgaagcg gaagaagaga agttcgactt cagcattctc gataaagtgg ttgaggaagc
    49441 gaataacgtt gatatccgcg aaatcgccca gcagaccgag caggaagtgg tggaagtgga
    49501 aactgtcaat ggcttcggcc cgaatgacgt catcctcgat atccgttcta tcgatgaaca
    49561 agaagataat ccactgaaag ttgaagggat tgatgtggtt tctctgccgt tctataaact
    49621 gagcaccaaa tttggcgatc tcgaccagaa cagaacctgg ctactgtggt gtgagcgcgg
    49681 ggtgatgagc cgtctgcagg cgctctatct gcgcgagcag ggctttaaca atgtgaaggt
    49741 atatcgcccg taatttgtgg ttgtaacgtc gcatctggtc agatgcgacg cttaccgcat
    49801 ccgacactac tcgtaataat tataaatccc tgccgccatc accagttgtg atgccacttc
    49861 atgggctttc tcgcgcccaa ccagcaggtc gataattttc agaccaaagt cgatagctgt
    49921 acccggcccc tggctggtca gcaattttac ccgtgcatcc cagacgacgc gcttgtcctg
    49981 ccattgttca gcgggaattt tgtctttcag cgtcgggaag ccggtcatat tgccaatcgg
    50041 gaagatatcg tgcggcacca gcacggtgac tggcgcggcg caaatagccg cgacgatacg
    50101 cccggaacgg tggaactgtt taacggtttc aaccagcagg gtactatcgc gaaaacactc
    50161 tgcgccttta atgccaccag gcagtacgat cacgtcatat tcgccatcag ccacctcgac
    50221 cagaggcgca tccgccaaca gcttcacgcc gcgcgagcag gtaattgcca ggttgccatc
    50281 gctggctacg ctggcagtgg tgactttgat accgccgcga accaagaggt cgatcgtggt
    50341 gacggcttca gtctcttcac taccaggggc gaggcaaacc agtgccgatg cgctcatatt
    50401 cactctcctt tctttttacc atttcaaaca ggcgggtgtt ttccggtacg gcaatcccat
    50461 gcgcgcgggc gcggcgtaag agaaaaccat tgatatagtc gatttcggtg tggcgcagcg
    50521 cgcggatatc ctgcaacatc gacgagatat tttccgctgt ggcatcaatc acctgcatca
    50581 cgtaatcacg caaatcttcc gctgaagtat gatgcccttc gcgttcgatc actgccgcga
    50641 cttcttcgca tatttgcata atttcttgcg gatgatgacg taattcaccg ttcgggcaat
    50701 tccagatggc agtcagtgga ttaatcacgc agttgacggc cagcttgcgc cacagctcgg
    50761 cgcgaatatt gttatgccag gcaacgtcag gcaacacggt ttgcaaaata tccgccagat
    50821 aactgtaatc gccatcctgt tgccgtgccg gaccgatatg cgtgattccg tttgccacat
    50881 gaataatgac attgccgtcg cggcgggcgg catgggtggt ggtgcccatc agtaatggct
    50941 gctgaatgtt ttgcaattct tcgatggtgc ccatgccgtt gtgaattaac agtattggcg
    51001 tagttacagg cagtgtggac gcgaggcttt tgacggcatc ggaaacctgc catgctttca
    51061 gcgtcaccag gagcagatcg ctggtggcaa gaaaatcggg atcgttggcg gtcagcgatt
    51121 cgttaaatat cgaaccatct gtctcaacca gattcacact acaataaggt tgcggtacgc
    51181 gcagccagcc ctgaacttcg tgaccctgtt tacaaagtgc tgtaagccat aattgcccta
    51241 aggcaccgca tcccaatacg gtaattttca ttgttcctcc tcacccgcaa ccactccggg
    51301 tgttcaataa ggctatccct taattgtgca tgctgttgcg actatgcaca attaagggat
    51361 acgtcctggt gcaggactgt tggttattta actttgcggg tattatgctt cgcatcaaaa
    51421 atgaagggag aggaaaagat gccatctttc gatattgtct ctgaagttga tcttcaggaa
    51481 gcacgtaacg cggtcgataa cgcgagccgc gaagtggagt cccgttttga cttccgtaac
    51541 gttgaagcct catttgagct gaatgacgcc agcaaaacca tcaaagtgtt gagcgagtcc
    51601 gatttccagg tcaatcagtt gctggatatt ttgcgtgcca agctgctgaa gcgcggcatt
    51661 gaaggcagtt cactggatgt accggaaaat atcgttcata gcggtaaaac ctggtttgtg
    51721 gaagcgaaac tgaaacaggg cattgagagc gcgacccaga agaaaatcgt caagatgatc
    51781 aaagacagca aactgaaagt gcaggcgcaa attcagggcg atgaaatccg cgtaacgggc
    51841 aaatctcgtg atgatttgca ggctgtcatg gcgatggtac gtggtggcga tctcggtcag
    51901 ccgttccagt tcaaaaactt ccgcgattaa tcgcgactat gtgcgttttg ttcatgtcag
    51961 atgcggcatg aacgcctgat tcggcctaca aaggtttgta attcagaaac tttgtaggcc
    52021 tgataagcgt agcgcagcag gcaattcgcc gcgtttttta tgcctgacga attgcctgtt
    52081 ctatctcaaa gcgattcgtc actttgctgt cgattttcac ataagctgaa tgttcttctt
    52141 ctgcaatcaa cacttctttg atgccttcag tttctagcaa acgcactttt aacgcctcgt
    52201 ttgcggcaat gttcgccggg atttcaatgc gcaaactgct gacatacggc ggttctttca
    52261 tggtactggc gactgtcagc cacactgcgg ccagcattgc gccagcgaga aataccccct
    52321 gaccgtcaaa catgccgtca atccagccgc ccagcgaacc gccaatcgcc acgccaagaa
    52381 actggctggt ggagtaaaca cccatcgccg tacctttgta acctgctggc gactctttac
    52441 tgataagtga gggcaggagg gcttccatca aattaaacgc cacaaagaaa agctgcacgc
    52501 cgaccaccag ttgccagaac tgcgtttgcg cgttccacaa cacaatttcc gcaaccacga
    52561 tcaacccgac gcagaagaca aagacttgct tcattttgcg cttaacttca gcgtagataa
    52621 tgaaaggcac gaccgagcca aaggcgatta gcattgtcgc cagatagacc ttccagtgtt
    52681 cagccgccgg gaaccctgca tcagccagtt gtccgggcag ggcaacaaac gtcgacatca
    52741 gcaaaatatg cagacacata atgccaaagt tgagtttcag cagccgcggt tccgccagca
    52801 ctttactgaa actgcctttc accattccgg actcacgatt aagtacgtga gtgctactgt
    52861 tgggcacaac ccaaatggtc aacgcaatgc cggtcgttgc cagaatagcg atcatccaga
    52921 acagcgcgtg cagcccaagt ttgtgagtga tgatcgggcc aagcaccatc gcaatggcaa
    52981 aggtaatgcc aaagctcacg ccgataaacg ccattgcttt ggtgcggttt tgttcgcgcg
    53041 tgagatcgga aagcagcgcc ataacggcag cggcaatcgc accggagcct tgtagcgccc
    53101 ggcccagaat aattccccag atggagtcag aaagcgcagc gataacgcta ccggcggcaa
    53161 acaccgccag cccaccgaca attaatggtt tgcgaccaat gcggtcggaa agcaggccaa
    53221 acggaatctg aaaaacggcc tgagtcagac cataaatacc aatggcaata ccgattaatg
    53281 cttcgctggc accttgcaga gccatgccat acgtggtcag aaccggcaga accataaaca
    53341 tgcccagcat gcgcaacgag aaaacggttc ctaaacccca ggtcgcgcgc cgctcacctg
    53401 gcgtcatttt ataatcgttc attaccacct ctgttttaaa ttcgcgacta gtgtaaagcg
    53461 gcaaacgaat ggggtaaaca tgcggatttt ttagtaaata tttcgagagg gatatgtttc
    53521 taatgctaag aaaaaaggtg ccgtagcacc tttttaactg agaggttttg ttaccacaca
    53581 gcagccagca gcgtatgcga gtccggtacc ataaaatcaa cggacatcat caccgagagg
    53641 gcagtgatgg cgatgataga gaagccgaac agcttgcgcg cccagattct gtcatcagca
    53701 actttataac cgcgcagagc catacctaac caccagacgc taaccgccgc ggcgaccacc
    53761 agatatttat atccagcgta accgccaaga gagagcatca gcgtggcaac ggcaaaggcg
    53821 atgatataca gcgtgatgtg attcttcgcc accgaaatgc cttttaccac tggcaatacc
    53881 ggaatgtttg ccgcctggta atccttaaag cggaaaatgg cgatggcata ggagtgaggc
    53941 atctgccaca ggctgaagat agccagcagg atcgctgcgc cgctatcgaa ctcgccggtt
    54001 actgcacagt agccaatcac cggcggcgca gcgccggaga gcgaaccaat caatgtgccg
    54061 tagactgagt ggcgtttcat gtacaggcta tagacgccga cataaaccac aaagcccatc
    54121 acccccagcc agcaggccag cggattcgcg ccaaaccaca gcagcataaa gccagcaata
    54181 cccagcaagg tggcgtacac cagcgagaca gcaggagaga tcaggccttt aaccagcacc
    54241 cgattcttcg tcctttccat ctttctgtcg atatccctgt cgatgtagtt gttaaacaca
    54301 caacccgacg ccacaaccag tgacacccca accagcgtgt agataaacaa gggataatca
    54361 atgctgccct ttgaggccag caggaatccc ccaatcaccg agatcaggtt gccaaagatg
    54421 atgcctggtt tcgttacttg caggtattgc ttaaacatca taaccgccgc tcttagtgca
    54481 tcatcatgtt gtagttgagg ttccacataa tccagatgga gcctacaacc aggatagcga
    54541 tgattagcac ggtgaagaca aacgccgtca tgttccagcc ttcatctgat ttggtattca
    54601 tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac tgccattgcc aggattgttc
    54661 ccagaattac ggccggagag gcagcccctg tcatcaccat ccagaacgga atcaccgtca
    54721 gaatgatcga caggataaag cctgtcatgt aggtttttac gctgccatgg gacgcgccgc
    54781 tgtgatcggt agaatgactc attacatcgc ccccatcaga taaacaacag tgaacacaca
    54841 gatccaaacc acatccagga agtgccagaa caggctcagg cacatgatgc gggtacggtt
    54901 agtgctggtc aggccgcgac gagcgatttg taccatcagc accgccatcc agataagacc
    54961 ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct
    55021 gcgatccgga cccatgccgt taacaatcag gtgatggaat tcgtagattt ccatcccgat
    55081 aaatccggca ccaaacaacc aggtcaacgc cagccaggag ataacctggc ttttgttgtt
    55141 tttgtacatg gcgatagccg ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt
    55201 ttcaaccaga acgaacggca gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc
    55261 gttcaccaga acggcatagg tagcaaacaa gattgagaac agaatgcagt cgctcatcag
    55321 gtagatccaa aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc
    55381 gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt cagccctgcc ttagtaatct
    55441 catcgaaatg ctggttttcc agtttttcga tttctgccac cggcacgtag taatccacgt
    55501 cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc tgcgaagcca acaatcgcca
    55561 gccaccagat atgccagatc atggcgaaac cgaagatggt ggagaaagct gcaatgacga
    55621 tacctgcacc gctgtttttc ggcatatgaa tttcttcata gtggtcaggc tttttatacg
    55681 cttcgccttt ctctttcatt tcccagaatg catcacgttc gtgaacgtgc ggcactacgg
    55741 caaagttata gaacggaggc ggggaagagg ttgcccactc cagcgtacgg ccaccccacg
    55801 ggtcgccagt caggtcacgg ttctggtcgc ggtcgcgaat agaaacgtac atctgaataa
    55861 cgaggcagag aatacccagc gcaatcagta ctgcaccgct ggctgcaatc atcagcatgg
    55921 tgtggaactg cgggtcaatc tgctggctca aacgacgggt catgcccatg aagcccagcg
    55981 catacagcgg cataaaggca acgaagaagc cgatgatcca gaaccagaac gcgcgtttac
    56041 cccaggtttc gttcagtttg aaaccgaacg ctttaggcca ccagtaggtc atccctgcga
    56101 agcagccgaa gaccacgccg ccgatgatca cgttatggaa gtgcgcaatc aggaacaggc
    56161 tgttatgcag aacgaagtcc gcgcccggta ctgccagcag cacgccagtc atcccgccca
    56221 ccgagaaagt gacgataaaa ccgatggtcc acagcatcgc agaatggaac acgatgcggc
    56281 cctgatacat ggtgaacagc cagttgaaga tcttcacccc ggtcgggatg gcgataatca
    56341 ttgtggtgat accaaagaag gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt
    56401 gcagccaaac gatgaacgac agcacggtga tacagacggt tgcccatacc agcgaggtat
    56461 aaccaaacag acgtttacgc gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag
    56521 gcaggatcag gatgtaaact tccgggtggc cccaggccca aatcaggttg atgtacatca
    56581 tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc cagatagcga tccagggtca
    56641 acaacgcgac ggtaaccgtc agaattggga aggaagcaat aatcagtacg ttcgcgcaca
    56701 gtgatgccca ggtaaatact ggcatcttga acatggtcat gcccggtgcg cgcatcttca
    56761 gaatggtaac gaagaagttg ataccggtaa gcgtcgtacc tataccggat agctggagac
    56821 tccatatcca gtaatcgaca ccgactcccg gactgtactc tattcccgat agcggtggat
    56881 aggccagcca gccggtctgc gcaaattcgc ccacgccgag agaaacgtta accagaatca
    56941 caccaacaac agtaaaccag aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg
    57001 cgccgatctg cagcggaacc accaggttca tcagaccgat aacgaaaggc atcgccacga
    57061 agaagatcat aatcacgccg tgcgcggtaa agatctgatc gtagtggtga ggtggcagga
    57121 agcccgcttc gcccgccgag gcaagagcct gctggctacg catcataatg gcgtcagcaa
    57181 aaccacgcag caacatcaca atcgccacga tgatatacat gataccgagg cgtttatggt
    57241 cgacggaggt cagccactct ttccacaggt aggtccactt accgaagtaa gtgatcaggc
    57301 caacgagcgc cagacctccc aaaataatgc cagcgatcgt aaccatgacg ataggttcat
    57361 ggaacgggac tgcatcaagt gataattttc cgaacatctt tattcttcct caaccccttt
    57421 aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat accttcgtgt gcgctgtgct
    57481 caccttctgg ctgggtcatg tccatgctct taccgtgagc cataaacttg ttaattacat
    57541 cggcaaacaa gtctggtttc acgttggaga aatattccac ctggttgtat tcgctaggcg
    57601 cggccagttt ttcgaacgca gccatgtcag acatggtgtt cggcgactgc ttcgcttttg
    57661 cgacccactg gtcgaatgcg gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg
    57721 agaagcccgg gccgctgtag ctggcggaga taccgtcata agtgccgggt tcgttggcga
    57781 tcagatgcag gcgagtctgc ataccggcca tggcataaat ctggctaccc agacgcggaa
    57841 tgaagaagga gttcatcacg gagttggagg tcactttgaa gtacaccgga gtgttcgccg
    57901 ggaaagcgat ttcattcacg gtagcaatgc cctgttccgg gtagatgaag aaccatttcc
    57961 agtccatgga aaccacttcg atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag
    58021 gctcaagagc gtgagtggtt ttccaggtca gtactgcaag gaagatgatg attaagatag
    58081 gtaccgtcca gaccacagct tccactttat tggagtgtga ccagttcggg ctgtacttag
    58141 catctttatt gctcgcacgg tacttccagg cgaaaccaac agccatcaag attgcgggaa
    58201 taacgacaat caacatcagg ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa
    58261 tctgtccttt gggatctaac agcgcagaat tacagccact gagcaataca gtgcctgcaa
    58321 ataatgacaa ccatcccaaa cttttattgt atttcctgag tctcatttaa cgacctcaat
    58381 tccacgggat ctggtggcgt ttaaagtgtg tgggcatttt acgggaaggt tacattactg
    58441 taaacatgat taaattcctg ttacttggat ttggctgcca ggtcacatat gttgcaaaac
    58501 atatcaggtt gattttaaga agctgttgca aaagggcgtt ataacaaaag gggagtgctg
    58561 aaggagtctg ggcgggcaat tggtataacc aatgtgaaat aaaataatta tttaacaaat
    58621 aattatcatt agagtgacaa ctgggtgatg agaaaataaa gactcattag ctgaatcgtg
    58681 taagaaaaat agaatttata aatggcgcca ataatttcca aacgctaagc cgcacaaaag
    58741 aacaaatatt aataagcgat actgatgttt atttagccgt aataattacg gcgagtgatt
    58801 actacagcta aataatattt acagattacg tcagatgcgt ttttcgtagc gccagataat
    58861 ccagcaaacc accaagcacg acgccagaaa gggcgactaa aaccccgact tccagcagag
    58921 caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat cgtcaatagc agtaaccaca
    58981 cggccaacag gctgacgccc gccgccagtg tccacatggc aaaggcataa cctgccggat
    59041 atgcggtacg ggagataaag ttgtcattta ctcgtgtata ttcaagcgtc tggcggcaaa
    59101 ccagcagcaa aataagccct ggtacggcag cggcgacgga gaatagatag aatgtcgacc
    59161 agccgtgtgc ttcaacaaac caacccgcca cggggccgac ataaactcgc cctacagcag
    59221 aaagcgctga gagcagggca aattgagtag cggaaaatga cttattacat agcgtcatta
    59281 acagcgcgac aaaggctgat gtgcccatcc cgccacagag gttttcgaaa aagacggctg
    59341 cgcccatgct gtagagatgc ttatcagtaa tcgacagcag ccagtaaccg gcgttagacg
    59401 caccttgtaa aatgccgaaa atcagcagtg cccggaacag tgacaggcgc tgcatcaaaa
    59461 tcccaccgta caatgcgcca acaatggtcg ctagtaagcc aagcgttttg ttaaccacgc
    59521 ctacttcacc cgcatcaaac ccgacgccgc gaatcaaaaa cgtggttgtc aggctcatgg
    59581 cgaatgcgtc gcccagctta tacagcacga taagaagcaa aataagccag gcattattgc
    59641 gaccaaagaa atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttt ggcacaggaa
    59701 tggtgtcggt cggttctggt gcaagcaacg tcgcgataat acaggggatc aacagcgccg
    59761 ccatcaacca gtacatgccc tgccagccca gccatttatc tgccagccac agggctaggc
    59821 cgccggaaac cagcatccct aaacggtaac ccagaacgct gattgccgcg cccgcaccac
    59881 gttcttctgc cgggagcaca tcggttttcc acgcatcgaa gacaatatcc tgggaggcag
    59941 agcaaaaagc gatcaccacc gccagcgccg ccatccagcg gagttgggtg cctggttcga
    60001 gaaaacccat cgccgcaatg gcgactaata acaggatttg cgtggcgagc agccaaccgc
    60061 gtcgccgccc aaaaaatgga ggcgtgtagc ggtccatcag cggtgaccag aggaatttaa
    60121 aaacgtaagc ctggcctacc agagagaaga aaccaatggt tttgagatca atattctcga
    60181 ccgtcatcca ggcctgtaag gtaccggatg tcagggcgag cggtagcccg gaagcaaaac
    60241 ccaggatcag caatatggct gaacgcggct gttgaaaaat acgtaaatat tgactggaca
    60301 tgggcgtcta caggcccggc ttgcaccggg ccagagggca gaaattaacg cgcgttctgc
    60361 ttgatgaatt cgtggatgct ggtgtcctga gacatatcag cgatggtatc ggtcagcaca
    60421 ctgttaaccg catcggcgat atttttgttg gaggcctgga acgcaccttc aacgttgtag
    60481 ctggcacgat agtttttggt cattttgttg ccattctgcg cggtagcgat gatggcgata
    60541 tccgctttgg tcgcgatgtt gtagcgcacg ttgccctggg acacgtcagc atacagttgg
    60601 ctaacgatga tttgcagatt aaccgggcca ttcggaccaa ccatgtaacc acgcgcggtc
    60661 atctgttttt ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc ggtcagggta
    60721 acgatttgat tatcgcgggt gacttttgcc agcgcctgat cggtacgctg atcggcacca
    60781 ttaatgctta cggtgacgcc catcaggctt ggatcctgct gtggcagtgt aatcgtcggg
    60841 gaaacttcaa tagttgttgg cggttttgcg catcctgcaa gcataaacag agcaactaac
    60901 gggaagagga tttttttgaa catgttcggg ctctcagaga ctcttaagcg tgtttggtaa
    60961 aaattcccgc catcataaca ttgccaacgg cgaggggaag tgggtaaggc atgtaaattc
    61021 atcatgttga cgaaataatc gcccctggta aaagaaacac tgatgcgagg cctgtgtttc
    61081 aatctttaaa tcagtaaact tcatacgctt gacggaaaaa ccaggacgaa acctaaatat
    61141 ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt atttaaaggg atggatgaca
    61201 tctcagcgtt gtcggaggag atatttcatg atgatacgtg agcggataga agaaaaatta
    61261 agggcggcgt tccaacccgt attcctcgaa gtagtggatg aaagctatcg tcacaatgtc
    61321 ccagccggct ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt tacgggtgaa
    61381 cgttttctga atcgtcatcg aatgatttac agtactttag cggaggaact ctctactacc
    61441 gttcatgcgc tggctctgca tacttacact attaaggagt gggaagggtt gcaggacacc
    61501 gtctttgcct ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg catttgcaac
    61561 tgtcggcgct tttccagtat gttgctaaag attttatgaa aaacggcctg cgggccgttt
    61621 tgttttgtct ggattttgct ctttttgccc agcattcaga cgaaaattgc ccgggaattg
    61681 tgaaaaaata cgcgacagcg cgcaataacc gttctcgact cataaaagtg atgccgctat
    61741 aatgccgcgt cctatttgaa tgctttcggg atgattctgg taacagggaa tgtgattgat
    61801 tataagaaca tcccggttcc gcgaagccaa caacctgtgc ttgcggggta agagttgacc
    61861 gagcactgtg attttttgag gtaacaagat gcaagtttca gttgaaacca ctcaaggcct
    61921 tggccgccgt gtaacgatta ctatcgctgc tgacagcatc gagaccgctg ttaaaagcga
    61981 gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca aagtgccaat
    62041 gaatatcgtt gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg gtgacctgat
    62101 gagccgtaac ttcattgacg ccatcattaa agaaaaaatc aatccggctg gcgcaccgac
    62161 ttatgttccg ggcgaataca agctgggtga agacttcact tactctgtag agtttgaagt
    62221 ttatccggaa gttgaactgc agggtctgga agcgatcgaa gttgaaaaac cgatcgttga
    62281 agtgaccgac gctgacgttg acggcatgct ggatactctg cgtaaacagc aggcgacctg
    62341 gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta accatcgact tcaccggttc
    62401 tgtagacggc gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg cgatgggcca
    62461 gggtcgtatg atcccgggct ttgaagacgg tatcaaaggc cacaaagctg gcgaagagtt
    62521 caccatcgac gtgaccttcc cggaagaata ccacgcagaa aacctgaaag gtaaagcagc
    62581 gaaattcgct atcaacctga agaaagttga agagcgtgaa ctgccggaac tgactgcaga
    62641 attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg
    62701 taaaaacatg gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt ctcaggcgat
    62761 cgaaggtctg gtaaaagcta acgacatcga cgtaccggct gcgctgatcg acagcgaaat
    62821 cgacgttctg cgtcgccagg ctgcacagcg tttcggtggc aacgaaaaac aagctctgga
    62881 actgccgcgc gaactgttcg aagaacaggc taaacgccgc gtagttgttg gcctgctgct
    62941 gggcgaagtt atccgcacca acgagctgaa agctgacgaa gagcgcgtga aaggcctgat
    63001 cgaagagatg gcttctgcgt acgaagatcc gaaagaagtt atcgagttct acagcaaaaa
    63061 caaagaactg atggacaaca tgcgcaatgt tgctctggaa gaacaggctg ttgaagctgt
    63121 actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc aacgagctga tgaaccagca
    63181 ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac cgccaggtgg
    63241 tgggcttttt tttgtcatga attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta
    63301 gcgtaacaac aaaagattgt tatgcttgaa atatggtgat gccgtaccca taacacaggg
    63361 actagctgat aatccgtcca taaggttaca atcggtacag caggtttttt caattttatc
    63421 caggagacgg aaatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg
    63481 gtgccgatgg tcattgaaca gacctcacgc ggtgagcgct cttttgatat ctattctcgt
    63541 ctacttaagg aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg
    63601 attgtggcgc agatgctgtt cctggaagcg gaaaacccag aaaaagatat ctatctgtac
    63661 attaactccc caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt
    63721 atcaagcctg atgtcagcac catctgtatg ggccaggcgg cctcgatggg cgctttcttg
    63781 ctgaccgcag gggcaaaagg taaacgtttt tgcctgccga attcgcgcgt gatgattcac
    63841 caaccgttgg gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt
    63901 ctgaaagtta aagggcgcat gaatgaactt atggcgcttc atacgggtca atcattagaa
    63961 cagattgaac gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac
    64021 ggtctggtcg attcgattct gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct
    64081 atacttatcc agggcggcac aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt
    64141 cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc catgacagat aaacgcaaag
    64201 atggctcagg caaattgctg tattgctctt tttgcggcaa aagccagcat gaagtgcgca
    64261 agctgattgc cggtccatcc gtgtatatct gcgacgaatg tgttgattta tgtaacgaca
    64321 tcattcgcga agagattaaa gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc
    64381 cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag
    64441 tgctggcggt cgcggtatac aaccattaca aacgtctgcg caacggcgat accagcaatg
    64501 gcgtcgagtt gggcaaaagt aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc
    64561 tgctggctga aacgctggcg cgcctgctgg atgttccgtt caccatggcc gacgcgacta
    64621 cactgaccga agccggttat gtgggtgaag acgttgaaaa catcattcag aagctgttgc
    64681 agaaatgcga ctacgatgtc cagaaagcac agcgtggtat tgtctacatc gatgaaatcg
    64741 acaagatttc tcgtaagtca gacaacccgt ccattacccg agacgtttcc ggtgaaggcg
    64801 tacagcaggc actgttgaaa ctgatcgaag gtacggtagc tgctgttcca ccgcaaggtg
    64861 ggcgtaaaca tccgcagcag gaattcttgc aggttgatac ctctaagatc ctgtttattt
    64921 gtggcggtgc gtttgccggt ctggataaag tgatttccca ccgtgtagaa accggctccg
    64981 gcattggttt tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc
    65041 tggcgcaggt tgaaccggaa gatctgatca agtttggtct tatccctgag tttattggtc
    65101 gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt cagatcctca
    65161 aagagccgaa aaacgccctg accaagcagt atcaggcgct gtttaatctg gaaggcgtgg
    65221 atctggaatt ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa
    65281 ccggtgcccg tggcctgcgt tccatcgtag aagccgcact gctcgatacc atgtacgatc
    65341 tgccgtccat ggaagacgtc gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa
    65401 gcaaaccgtt gctgatttat ggcaagccgg aagcgcaaca ggcatctggt gaataattaa
    65461 ccattcccat acaattagtt aaccaaaaag gggggatttt atctcccctt taatttttcc
    65521 tctattctcg gcgttgaatg tgggggaaac atccccatat actgacgtac atgttaatag
    65581 atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg aaattaaact aagagagagc
    65641 tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg
    65701 gtggtttatc cgcacatggt catcccctta tttgtcgggc gggaaaaatc tatccgttgt
    65761 ctggaagcgg cgatggacca tgataaaaaa attatgctgg tcgcgcagaa agaagcttca
    65821 acggatgagc cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc tatattgcag
    65881 atgctgaaac tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt
    65941 atttctgcgc tctctgacaa tggcgaacac ttttctgcga aggcggagta tctggagtcg
    66001 ccgaccattg atgagcggga acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa
    66061 ggctacatca agctgaacaa aaaaatccca ccagaagtgc tgacgtcgct gaatagcatc
    66121 gacgatccgg cgcgtctggc ggataccatt gctgcacata tgccgctgaa actggctgac
    66181 aaacagtctg ttctggagat gtccgacgtt aacgaacgtc tggaatatct gatggcaatg
    66241 atggaatcgg aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag
    66301 cagatggaga aatcccagcg tgagtactat ctgaacgagc aaatgaaagc tattcagaaa
    66361 gaactcggtg aaatggacga cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac
    66421 gcggcgaaga tgccgaaaga ggcaaaagag aaagcggaag cagagttgca gaagctgaaa
    66481 atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga ctggatggta
    66541 caggtgccgt ggaatgcgcg tagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc
    66601 cttgataccg accattatgg tctggagcgc gtgaaagatc gaatccttga gtatcttgcg
    66661 gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg gccgccgggg
    66721 gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca ccgggcgtaa atatgtccgt
    66781 atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc
    66841 ggttctatgc cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg
    66901 ttcctgctcg atgagatcga caaaatgtct tctgacatgc gtggcgatcc ggcctctgca
    66961 ctgcttgaag tgctggatcc agagcagaac gtagcgttca gcgaccacta cctggaagtg
    67021 gattacgatc tcagcgacgt gatgtttgtc gcgacgtcga actccatgaa cattccggca
    67081 ccgctgctgg atcgtatgga agtgattcgc ctctccggtt ataccgaaga tgaaaaactg
    67141 aacatcgcca aacgtcacct gctgccgaag cagattgaac gtaatgcact gaaaaaaggt
    67201 gagctgaccg tcgacgatag cgccattatc ggcattattc gttactacac ccgtgaggcg
    67261 ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgtc gcaaagcggt taagcagtta
    67321 ctgctcgata agtcattaaa acatatcgaa attaacggcg ataacctgca tgactatctc
    67381 ggtgttcagc gtttcgacta tggtcgcgcg gataacgaaa accgtgtcgg tcaggtaacc
    67441 ggtctggcgt ggacggaagt gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg
    67501 ggtaaaggca aactgaccta taccggttcg ctcggcgaag tgatgcagga gtccattcag
    67561 gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac
    67621 gaaaaacgtg acatccacgt ccacgtaccg gaaggtgcga cgccgaaaga tggtccgagt
    67681 gccggtattg ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc
    67741 gatgtggcaa tgaccggtga gatcactctg cgtggtcagg tactgccgat cggtggtttg
    67801 aaagaaaaac tcctggcagc gcatcgcggc gggattaaaa cagtgctaat tccgttcgaa
    67861 aataaacgcg atctggaaga gattcctgac aacgtaattg ccgatctgga cattcatcct
    67921 gtgaagcgca ttgaggaagt tctgactctg gcgctgcaaa atgaaccgtc tggtatgcag
    67981 gttgtgactg caaaatagtg acctcgcgca aaatgcacta ataaaaacag ggctggcagg
    68041 ctaattcggg cttgccagcc tttttttgtc tcgctaagtt agatggcgga tcgggcttgc
    68101 ccttattaag gggtgttgta aggggatggc tggcctgata taactgctgc gcgttcgtac
    68161 cttgaaggat tcaagtgcga tataaattat aaagaggaag agaagagtga ataaatctca
    68221 attgatcgac aagattgctg caggggctga tatctctaaa gctgcggctg gccgtgcgtt
    68281 agatgctatt attgcttccg taactgaatc tctgaaagaa ggggatgatg tagcactggt
    68341 aggttttggt acttttgccg ttaaagagcg tgctgcccgt actggccgca acccgcagac
    68401 cggtaaagag atcaccatcg ctgctgctaa agtaccgagc ttccgtgcag gtaaagcact
    68461 gaaagacgcg gtaaactaag cgttgtcccc agtggggatg tgacgaagtt caagggcgca
    68521 tctactgatg tgcctttttt atttgtattc ggtgactttc tgcgtcttgt gggctgacaa
    68581 ttgcccccgt ttcttgtcac aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca
    68641 cagtcatcta cagcggagtg ttgttacacc atgatggaca gcttacgcac ggctgcaaac
    68701 agtctcgtgc tcaagattat tttcggtatc attatcgtgt cgttcatatt gaccggcgtg
    68761 agtggttacc tgattggcgg aggcaataac tacgccgcaa aagtgaatga ccaggaaatc
    68821 agccgtgggc aattcgagaa cgccttcaac agcgagcgta atcgcatgca gcaacagctg
    68881 ggcgatcaat actccgagct ggcagcgaac gaaggctata tgaaaaccct gcgtcaacag
    68941 gtgctgaatc gtctgatcga cgaggcgctg ctggatcagt acgcacgtga gctgaaactg
    69001 ggtatcagcg atgagcaggt taaacaggcg attttcgcga ccccagcctt ccaggttgac
    69061 ggcaaatttg ataacagccg ctataacggt atcctcaacc agatggggat gaccgccgat
    69121 cagtacgccc aggcgctgcg taaccagctc actacccaac agctgattaa cggcgttgcc
    69181 ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg cggcactggt cgcgcaacaa
    69241 cgcgtggtgc gtgaggcgac tatcgatgtt aacgcgctgg cggcgaagca gcctgtgacc
    69301 gaacaggaga ttgccagcta ctacgaacaa aacaaaaaca atttcatgac gccggaacaa
    69361 ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg
    69421 gatatccaga gctactacga ccagcatcag gatcaattca cccagccgca gcgtacccgc
    69481 tacagcatca tccagaccaa aactgaagat gaagcgaaag cggtacttga tgagctgaat
    69541 aaaggcggtg attttgctgc attagccaaa gaaaaatctg ccgatattat ctctgctcgt
    69601 aacggcggcg atatgggttg gttagaagat gccactatcc cggacgaact gaaaaatgct
    69661 ggtctgaaag aaaaaggcca actgtctggt gtcatcaaat cttcggtcgg tttcctgatt
    69721 gtacgtctgg acgacattca gccagcgaaa gtgaaatcgt tagacgaagt acgtgacgac
    69781 attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt actacgcgct gcagcagaaa
    69841 gtgagcgatg cggcaagcaa cgacaccgag tctctggccg gtgcagagca agctgccggc
    69901 gttaaagcca ctcagacggg ttggttcagc aaagataacc tgccggaaga gttgaacttc
    69961 aagccggttg ccgacgctat ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc
    70021 atcaactctg acatcatcac cgtagacggc gaccgcgcat tcgtgctgcg catcagcgag
    70081 cacaaaccgg aagcggtgaa accgttggca gatgttcagg aacaagttaa ggcattggtt
    70141 cagcacaaca aagctgaaca acaggcgaaa gtggatgctg agaaactgct ggttgatttg
    70201 aaagccggca aaggtgcgga agctatgcag gctgccggtc tgaaatttgg cgagccgaaa
    70261 accttaagcc gttccggtcg tgacccgatt agccaggcgg cgtttgcact gccactgcca
    70321 gcgaaagaca aaccgagcta cggtatggcg accgatatgc aaggtaatgt ggttctgctg
    70381 gcgctggatg aagtgaaaca aggttcaatg ccggaagatc agaaaaaagc gatggtgcag
    70441 ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa
    70501 gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca
    70561 aaaaaatgcg ttgcagctgt aacaagaaaa ggtcgctttc gcggcctttt ccatttctga
    70621 acattgccat ttgtttactg ttttcactgc cgttaaggtg attccactgt taacaaacaa
    70681 ggagaaaaca gtatgaaaca cggaattaaa gcactgctca ttaccctgtc cctggcctgt
    70741 gccggaatgt ctcatagcgc gctggcggca gcttctgtgg cgaaaccgac ggcggtagaa
    70801 accaaagcgg aagctcctgc agcacaaagt aaagcagcag taccggcgaa agccagtgac
    70861 gaagaaggca cccgggtcag cattaataat gccagcgcgg aagagctagc ccgcgcgatg
    70921 aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt atcgcgaaga gtacggtccg
    70981 tttaaaactg tggaggatct aaagcaggtg ccggggatgg gcaattcgct ggtggaacgt
    71041 aatctggcgg tattaaccct gtaattaatt tgcatagtgg caattttttg ccagactgaa
    71101 gaggtcatac cagttatgac ctctgtactt ataacaacaa cgtaaggtta ttgcgctatg
    71161 caaacacaaa tcaaagttcg tggatatcat ctcgacgttt accagcacgt caacaacgcc
    71221 cgctaccttg aatttctcga agaagcccgc tgggatgggt tggaaaatag cgacagtttt
    71281 cagtggatga cggcccataa catcgccttc gtcgtggtca atatcaatat taactatcgt
    71341 cgcccagcgg tattaagtga cctgttaact attaccagtc agttgcagca attaaacggt
    71401 aaaagcggca tcttaagcca ggtcattaca ctggagccgg aagggcaggt ggtagcggat
    71461 gcgcttatta cgtttgtttg tattgatctt aaaacgcaga aagcattagc tctggaaggg
    71521 gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt tttgtggtgc cggatgctca
    71581 agccgcatcc ggcgacaccc ggaataatta cctcaacccg gttttctgct tcatcgctgc
    71641 catcaccgtc ggtttatcgg ccagataatg attcaaaccg ttggcgcgta aattacatgc
    71701 cgcacaatga ccgcaaccgt cgcctttaaa gccgttatag caggtcaacg tttcgttacg
    71761 gactaaatcc agtttgccgt aataatctgc cagcgcccag gtttctgctt tatcaatcca
    71821 catcagcggc gtttcaaaac gaatatcttt cgccatgccc aaactgacgg catggtttag
    71881 tgctttcaca aactcatcgc ggcaatccgg gtagccggag aaatccgttt cgcagacgcc
    71941 agtaattacg gcttctgctt ttacctgata cgcatatatt gccgccagcg tcaggaacaa
    72001 aatattacgc cctgggacaa acgtattcgg gataccatcg gcttcaggtt cataatcagg
    72061 caccggaatg ctgtcacgcg tcaggctact gaccgccagc tcgttgagca gggtgacatc
    72121 cagcacctta tgcgcgcgtg cccccagttt cagcgccagt tcgcgtgcca cgtcgatttc
    72181 tgcgcgatgc cgctgaccgt aatcgaacgt cacgcaatgg acttcatcat attgttgtaa
    72241 tgcctgcacc agacaggtgg tggaatcctg acctccactg aacacaacga cagcacgttt
    72301 catagataat cctgcttgaa caataaaagc gttatggtaa cgcctgcgat taacccggac
    72361 cagctattca ttgcgatggc gcgggaagcc aggcttcggt aaattcaaac cagccgcgcg
    72421 gtgtcagtcg cacaccgttc acgccgggag gggcactaat gcgatagtga tagttgaaca
    72481 gcggcgtcag cgtcgcatct gtcattaact ggctaaaaac cgctttcagg gcattaaatc
    72541 ggttttcttc atcaggcatt atttgcacgg catccagtgt cgattgtaga tgtgcgtatg
    72601 ctggagcgtc gaaaacatgt ggccacagcg gatcgcagcg cagccattgc tccagagtat
    72661 attccggtgc ttcgccaatt aatctgtcgc ccatcatgag gtctgcgtgt gcctgtaggg
    72721 tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt gagttcacag ccttccgctg
    72781 ccagtgtcgc ctgtaggcgt tctgccatgg tatgaagttc tatcggtagg tgataaacca
    72841 gcgtcaaggt tttcggtagt ttgacttcat ccggtacctg ccaatgcgga atagtccagc
    72901 ctggcagtaa tgcatgactg gcggtgatca ggttttctcc gacttctaac gtttgtaata
    72961 aaccggattg atgaataatg gagatcactt ttcgcgcctg ccagagggag agtcggggac
    73021 ttttgcgcaa cgtcaaatag caaaaaccta aactgatgcc gctactgacc tggctgaccc
    73081 gttgcagctc ctccggtttg ccgatggtga tttgcacggg atgccgacaa ctggttccca
    73141 aatctttttc gaaaagcggc ggagttatcc agtactcaac cgctttaagc agcggatgac
    73201 gtaaatggta ataatcatgg ctttccaggc gcaccagctc tgctgtgaat tgtgttaagc
    73261 gaaaaggacc cgtgccgatc agtgggaatt gcggatgcgc cagatggctg caatagctcg
    73321 ccagccggtg cgcaagccag taatcagggc gatgtaaaaa gaaggtcaga cactgcggat
    73381 gggtgacttc aatacgcttc acgctaataa ataattgatc cagtgctggc agttgtaaca
    73441 gcatcaataa tcgctggtgt aagtgtgagg cttttactgc atcgccgtta tgccagtgta
    73501 gggttgaacg aagataaaag tcccagcgta acccgtcagt agaggtttcc cagtgatgcg
    73561 ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg ggtcaggccg gaaaatatct
    73621 gcccggcgag atgctgctcg gcacggccgg gcaaaaagcc tggttgtagc ggttcgagcg
    73681 ggcgatagta gggaatacgc aacgtgggtg tatcgttttg ccattgtccg cccataaacg
    73741 gctgtaacag agtgcgcagc tcacctgggg ccagttgcgc cagctccagc acatcttgct
    73801 gctttccggt ttccagtgcc tgttccatca tcgcattgcg tagcgattcc ggcgtgacca
    73861 gaaagcgtaa ttgtccgcgc tttccgcgtc ctgactgcgc ctgccactcc agccatcccg
    73921 cctcctgtgc ctgacgcaac agcgtacgaa catggcgttc gctgcaaaaa cagcgttcgg
    73981 ccagttcgct gacggtgacg gtttgcggct ttccggcgga aggttgccac agacgttgat
    74041 actggttaag acggttgagc aatcgcatat aaacccggaa caatattatt taactattca
    74101 ctattacttc cgtatatatc aggtgatact caatcaccat taaccgtgtc acagagtgga
    74161 gaaagaaatg gctcgtctgg cagcatttga tatggatggc actttattga tgcctgacca
    74221 tcatttaggt gagaaaaccc tctctacttt ggcgcgactg cgtgaacgcg acattaccct
    74281 cacttttgcc acggggcgtc atgcgctgga gatgcagcat attctcgggg cgctatcgct
    74341 ggatgcgtat ttgattaccg gcaacggaac gcgcgtgcat tctctggaag gtgaactttt
    74401 acatcgtgat gatttacctg cggatgtcgc ggagctggtg ctgtatcagc aatgggatac
    74461 ccgagccagc atgcatatct tcaatgacga cggttggttt accgggaaag agatccctgc
    74521 gttgttgcag gcatttgtct atagcggttt tcgttatcag ataatcgatg tcaaaaaaat
    74581 gccactcggc agcgtcacca agatctgctt ctgtggcgat cacgacgatc ttacacgctt
    74641 gcagatccag ctatacgaag cattaggcga gcgtgcacat ttgtgttttt ccgccacgga
    74701 ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct gcattgacgg tgctgaccca
    74761 acatttaggt ttatcgttgc gcgattgcat ggcctttggt gatgcgatga acgatcgcga
    74821 aatgttagtc agcgtcggta gcggatttat tatgggcaat gcgatgccgc aactgcgcgc
    74881 ggagctcccg catttaccgg tgattggaca ttgccgaaat caggctgtct ctcactattt
    74941 gacgcactgg ctggactatc cacatctacc ttattccccc gaataacgag atcccttcca
    75001 gcaccgggca attgcccggt tttttttgcg ttgaatttgt cattttgtgc cgtggtgttt
    75061 aaaccgcaca gaataaattg tcgtgatttc acctttaaaa taaaattaaa agagaaaaaa
    75121 attctctgtg gaagggctat gttagataaa attgaccgta agctgctggc cttactgcag
    75181 caggattgca ccctctcttt gcaggcactg gctgaagccg ttaatctgac aaccacccct
    75241 tgctggaagc gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg
    75301 ctggatccgg aaaaaatagg cctcggcctg accgcttttg tgctgataaa aacgcaacat
    75361 cacagcagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg
    75421 gggttctggc gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg
    75481 aaacgctacg acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc
    75541 acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata
    75601 tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc
    75661 gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc
    75721 aactggttcc gccaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta
    75781 ctaccgggca gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatc
    75841 tcctgcgtta cgtctggcgg gtattgctgt ttggtgcgtc ttatcaactg gctgttgaac
    75901 tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc tgagttttac ctgcgtcatc
    75961 gcaccggtga cctcatggct cgtgcgacca atgacgtcga tcgcgtcgtg tttgccgccg
    76021 gagaaggggt gctgacgctg gtggattcac tggtgatggg ctgcgctgtg ttgattatga
    76081 tgtctacgca aattagctgg cagttgacct tattttccct gttgccgatg ccagtgatgg
    76141 cgatcatgat taagcgcaac ggcgatgctt tgcatgaacg ctttaagctg gcacaggcgg
    76201 cgttttccag tcttaatgac cgcacccagg aaagcctcac cagtatccgc atgatcaaag
    76261 cctttggtct ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca
    76321 aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccgaccatc tatatcgcga
    76381 ttggtatggc gaacttgctg gcgattggcg gcggtagttg gatggtggtg cagggcagtt
    76441 taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc
    76501 tggcgctggc atggatgttt aacattgtgg aacgtggtag tgctgcgtac agccgtattc
    76561 gcgcgatgct ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc
    76621 gtggcgaact ggatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc
    76681 tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatctgc gggccgactg
    76741 gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtc agcgaggggg
    76801 atattcgctt tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc
    76861 tggcggtagt tagccagacg ccattccttt tttctgacac tgtggcgaat aacatcgcgc
    76921 tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac
    76981 atgacgatat tttgcgtcta ccgcaaggtt acgatacaga ggtgggcgag cgcggtgtga
    77041 tgctttccgg cgggcaaaaa cagcgtatct ccattgctcg tgcgttatta gtcaacgcgg
    77101 aaatcctcat ccttgatgat gcgctttcgg cggtggacgg acgcactgag caccagatcc
    77161 tgcataacct gcgtcagtgg gggcagggaa gaacggtaat catcagtgcc catcgccttt
    77221 ctgcactgac ggaagccagt gaaattattg tgatgcagca cggacatatc gcccagcgtg
    77281 gcaatcatga tgtgctggca caacaaagcg gctggtatcg cgatatgtat cgctatcaac
    77341 aactggaggc ggcgctcgac gacgctccgg aaaatcgcga ggaggccgtc gatgcgtagt
    77401 tttagccaac tgtggccgac tctcaagcgc ctgttagcgt acggttcgcc gtggcgtaaa
    77461 ccgctgggga ttgcggtcct gatgatgtgg gttgcggcgg cggcagaagt cagtgggccg
    77521 ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaagtg
    77581 gttgcagggc tggctgcggc gtatgttggg ctgcaactgt ttgccgccgg gctacattac
    77641 gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac
    77701 gtgatggatg ctgcgttacg ccagccatta agcgagtttg atacccaacc cgtcgggcag
    77761 gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg
    77821 gcaactgtcc tgcgcagtgc cgcgctggtg ggcgcgatgc tggtggcgat gttcagcctc
    77881 gactggcgaa tggcactggt ggcgataatg attttcccgg tggtgctggt ggtaatggtg
    77941 atataccagc gttacagcac gccgattgtc cgtcgtgtgc gcgcctatct ggcggatatc
    78001 aacgacggct ttaacgaaat catcaatggc atgagcgtta tccagcagtt tcgtcagcag
    78061 gcgcgatttg gcgaacgtat gggggaggcc agtcgttcac actatatggc gaggatgcaa
    78121 accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt
    78181 ctttgtggct tgttgatgct gtttggcttc tccgccagcg gcaccattga agtgggcgtg
    78241 ctgtatgcgt ttatcagcta tcttgggcga cttaacgaac cattaatcga actgaccacg
    78301 caacaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga actgatggac
    78361 ggaccgcgcc agcaatatgg caatgatgat cgcccgttac agagtggcac catcgaagtc
    78421 gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct
    78481 gtgccttcgc gcaattttgt ggcgctggtc gggcataccg gcagtggcaa aagcaccctc
    78541 gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt
    78601 ccattaagtt cgctaagtca cagcgcgctg cgccagggcg tggcaatggt gcagcaagat
    78661 ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc tggggcggga tatctccgaa
    78721 gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg agctggcgcg tagcatgagc
    78781 gacggtattt acacgccgct gggcgagcag gggaataatc tctcagttgg gcaaaagcaa
    78841 ctgctggcac tggcgcgcgt gctggtcgag acgccgcaaa tcctgatcct tgatgaggca
    78901 accgccagca ttgactccgg tactgaacag gcgattcaac atgctctggc ggcggtgcgt
    78961 gaacatacca cgctggtagt gattgctcac cgcttatcga ccattgttga tgccgacacc
    79021 attctggtgc ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca actgctggcg
    79081 gcccagggac gctactggca gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc
    79141 agcgtgcgtg aagaggaatc attgagcgcc tgaatagcgc aatatttcat cgttggtgca
    79201 aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaaactt cttaacttcc
    79261 tgctctcttt ctcgtttttc atttctggca caccgcttgc aataccttct tcgtgtagca
    79321 gaaccattac cgaattctga ccggagggga tctatgaagc tggtgaccgt gataatcaaa
    79381 ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc
    79441 gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg
    79501 gaatacagcg tcaatttcct gccaaaagta aaaattgatg tggcgattgc tgatgaccaa
    79561 ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc
    79621 aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa
    79681 gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa
    79741 aaactgggct tgcttcactg gcgatgcttc cgggactggt aatggctgca cctgcggtgg
    79801 ccgataaagc cgacaatgcg tttatgatga tttgtactgc gctggtgctg tttatgacta
    79861 ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc
    79921 tgacgcaggt gacggtgaca tttgcactgg tctgtattct ctgggtggtt tacggttact
    79981 cgctggcgtt tggtgagggc aacaacttct tcggcaacat taactggttg atgctgaaaa
    80041 acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgtg gcgtttcagg
    80101 gatcgtttgc ctgcattacc gtcggcttga tagttggggc gctggcggaa cgaatccgct
    80161 tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcttacatt ccgattgcgc
    80221 atatggtgtg gggcggtggt ttgctggctt ctcacggtgc gctggatttc gcgggtggca
    80281 ccgtggtgca cattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggaaaac
    80341 gcgtgggctt cggtaaagag gcgtttaaac cgcacaacct gccgatggtc ttcaccggga
    80401 ctgccattct ctatatcggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg
    80461 aaatcgcggc actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct
    80521 ggatcttcgg tgaatgggcg ctgcgtggta agccttcact gctgggggcg tgttctggcg
    80581 cgattgccgg tctggtcggc gtgacgccag cctgcggcta cattggggtt ggcggcgcgt
    80641 tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttaccatg ctcaaacgct
    80701 tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg
    80761 gctgtatcat gaccgggatt tttgccgcca gctcgctggg cggcgtgggc ttcgctgaag
    80821 gtgtgacgat gggccatcag ttgctggtac agctggaaag catcgccatt acgatcgtct
    80881 ggtccggtgt tgtggcattt atcggctaca aattggcgga tctgacggtt ggtctgcgtg
    80941 taccggaaga gcaggagcga gaagggctgg atgtcaacag ccacggcgag aatgcctata
    81001 acgcgtaaca agcactgcaa aaaacagccg gacggttttc acctccggct atttttttaa
    81061 ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa ccagtacgcc gtcttgggta
    81121 taaaactcac cgcgcacaaa gccacgtgcg ctggacgccg aggtgctctc cacgctatac
    81181 agcagccatt cattcaaatt aaacgggcga tggaaccaca tggaatggtc aatggtggca
    81241 atctgaatcc ccggttcgag aaaaccgatg ccgtgcggct gtagagctac cggcaggaag
    81301 ttaagatcag aagcgtaacc gagcagatac tgatgaacgc gcaggtcatc cggcacgcta
    81361 ccatttgcgc ggatccacac ctgacgatgt ggttctgcga cgtgaccttt cagtgggtta
    81421 tgaaactcca ccggacggac ttccagcgga cgatcgcaga tgaatttatc tttcagcact
    81481 ggcggcagca ggtgcgccag cgattgggcg atttgcgttt ccgaaggtag gccatcaggc
    81541 gctggcgcgg acggcattgt tttttgatgt tcgaaacccg cttctggtgc ctggaaagag
    81601 gcagtcatat aaaaaatcgg tttgccgttt tgaatagcag caacccggcg ggcgctgaag
    81661 ctgttaccgt cacgcagcgt ttcgacatca taaataatcg gcttcttact atcgccaggg
    81721 cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt cagggacggt ctcttttgca
    81781 gcatacaagg cctgacccac gacctggccg ccaaacacct ggcgtaaacc taaatcttca
    81841 ctctggccgc gaaagagtcc ttcctcaatt ttttccagat ttaacaatgt cagtaaattt
    81901 tttagcgcct gactcatata actctccagt aacaaagctg ccgcagcaag ccaaagtgag
    81961 ttgagtataa cgcaaatttg ctactggtcc gatgggtgca atggtctgaa ttacgggcta
    82021 attacaggca gaaatgcgtg atgtgtgcca cacttgttga cgttactatt ttgttaacca
    82081 ctcttccggc gaggaaagtt agcccgctgg tgcattgata ataaggagaa atgaatgaaa
    82141 ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt tggcggcttg cgcagataaa
    82201 agcgcggata ttcagacgcc agcaccggct gcaaatacgt ctatttcagc aacacaacaa
    82261 ccagctatcc agcaaccgaa tgtctccggt accgtctgga tccgtcagaa agtcgcactg
    82321 ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt cgttagccga tgcaccgtca
    82381 aaagtgctgg cgcagaaagc ggtgcgtact gaaggtaaac agtcaccatt cagctttgtt
    82441 ctgccattta acccggcaga tgttcagccg aacgcgcgta ttctgttgag tgcggcgatt
    82501 accgtgaatg acaaactggt atttatcacc gataccgttc agccggtgat caaccagggc
    82561 ggaactaaag ccgacctgac attggtgccg gtacagcaaa ccgccgtgcc ggttcaggcc
    82621 agcggtggcg caacgactac cgtaccttcg acttcaccaa ctcaggtgaa tccgtcttcg
    82681 gcagttccag ctcctacgca atattaagcg acgttaaccc tctccgcccg gagagggtta
    82741 gtagttccag cgataacgct gcaagtcgat ttgcccgctt cccgatacca tcacaccttc
    82801 tgccagtaat gcctgtcgct gacgctgtaa atccggtccg gttagcgaaa ttgtgccgtg
    82861 gcgattaacc acccggtgcc agggtaaggt gctgccttca gggagacgct ttaacacacc
    82921 gcccacctgg cgcgcggcgc ggggcgatcc cgccagtttc gccacatcac cgtaagtggt
    82981 gacatagcct tcgggaatag cggcgacgat ttgccagacg cgttggggaa atgaatcttc
    83041 tttttccatc ttttcttcct gaggtaattt ttcagcataa tctggaaaaa cgcccgagtg
    83101 aagtcgcatt gcgcaagaaa ccagcatctg gcacgcgatg ggttgcaatt agccggggca
    83161 gcagtgataa tgcgcctgcg cgttggttct caacgctctc aatgggggct ctgttggttc
    83221 tcccgcaacg ctactctgtt taccaggtca ggtccggaag gaagcagcca aggcagatga
    83281 cgcgtgtgcc gggatgtagc tggcagggcc cccacccatt tctgcctccc accgtttcgt
    83341 caaaaaatcc caacatggct aaactttaac cacaactgac gtcgcaagaa ttgtctggct
    83401 gcgcagtacg cttcggaggt atgtctgatg aagtatgttg atggttttgt ggttgccgtt
    83461 cctgccgata aaaaggatgc ctatcgggaa atggccgcta aggctgcgcc attgtttaaa
    83521 gagtttggcg cgcttcgtat tgtcgaatgc tgggccagcg atgtaccgga tggcaaagtg
    83581 accgattttc gtatggcggt gaaagcggaa gagaatgaag aggttgtctt tagctggatt
    83641 gaataccctt caaaagaggt ccgcgacgct gctaatcaaa agatgatgtc ggacccacgg
    83701 atgaaagagt tcggcgagtc catgccgttt gacggcaagc gaatgatcta tggcggattc
    83761 gagtcaatca tcgacgaata ggtagcgtga cgggctgtgc ggcgtgccgt cagcccaaaa
    83821 attcacaaat gttgctcggc ccagcgtaag aaatcttctt tcggtaatgc cttgctgtag
    83881 agccagccct gaccgtagtg cacgccatgc tggcgtaacc actcttcttg tttactggtt
    83941 tcgattccct ccgctaccat tttcagtttc agtgtttttg ccatttcgat gatatgcggc
    84001 gtgacatttt tatattccag cgcatcaacg aaagatttat cgatcttcag aatgtcgaca
    84061 tccagatcct gtaaataact taaacttgaa taccccgtac caaaatcatc aagataaatt
    84121 tcatggcccg cctcccggta gcgagaaatt atcggggcgc tggttttcgg atcggcaaac
    84181 tcgcgttcag tgagttcaag cgcgatctgt ctgggattaa cctgatagtg attgatcatg
    84241 tcacgcagca attgcgggat tttttccgag gtgagcacgg gggattcaag attgatcgaa
    84301 atatgctgct gtggatgctg acgcagccag tcgcccatat cttcaaagac gcttcttata
    84361 atcagtagcg tcaatggctc agaaaggccc gtttgctgtg ccagcggaat aaaactatct
    84421 ggtgacaacc aactaccgtc tgtctgcggc cagcgcgcca gtgcctcagc accgacaatt
    84481 ttgccattgg ctaaggagac aatcggctga tagtgcacgc aaatatcacg attttcgata
    84541 gcatcctgca gccgatgatg cggtgactga atacggcgca aaatacgcag cacaaacatc
    84601 gctgccagca ggccaatcac caacccgagc ggtaaccaaa taaagacttg ccgatgccag
    84661 cctttctgca acattttcgt tgaagcccat gtgatgatcg aaatattcat ctccggtaag
    84721 ggcaggatat cgtaaatgat tccattattt tcgatatgct caccgggtgt tttttgtagc
    84781 ctggtaataa ttccctgagc aatttcatcg ctgctggtta tgacaacgtt atgggcattg
    84841 ccaataatgg cggcatcaat ttgccatgag ctatagggaa tgacatcaat aaaggaagcg
    84901 gggtcgatca tgacgacata atgtgccgtt cccatggcga ccatgtaacg gataatgcct
    84961 aaatcgttat gcgatgttaa ccagacacga taaccatctt tcgaaatttt acctggctcg
    85021 gggaaggtat cgggcggact ttcatgctcc agagacgaac actggggaac gttgttatcg
    85081 atataagcca cttcctgaat atagcgataa ctgtaagata cccgacgcat ttccatgaga
    85141 tgggcttcgc tacaggcagc gccttgccat ctttccagct cctgcagcgc atctttccct
    85201 tgtgtcgcca ccttattggc tcgaatagcg acgcgggagg aataggtatc cagctcttca
    85261 ataaacgatg tttctacctg ctgatgggcc agccagatgc ttaagccgac aggcagcaat
    85321 actgaaagaa tcagtactcc cgaaataagg ccgaccagat gtcgtgttct caccagcata
    85381 tccttattaa cacagtgtag ggaaagtata tctgattgtg gaaaaaggct ggctggagat
    85441 aaggaggtag agctggtatt tcatgaagtg atgggtggaa acgatcattg tcatttgacc
    85501 aggcgcagag ctttacgcct ggtatgtaac cctaacttaa gatgttgatt cggcgcgagc
    85561 gagggtaaaa atatcgtaaa aagacagttc acctttacgg acgatcattt tttgcagttg
    85621 ttccttatga acatccgcga cacgcggaga gttcaagatg gcttcaacca gcgcggcagg
    85681 tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg cggaaatcca gcacgtcgat
    85741 atcttcatag cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa gcaggataaa
    85801 taatacaacc agtagccaga cagagcctga cagcgtttcg gactgcaaca tcaccgccag
    85861 ggtggcaaaa aaagcaacgt acataccgat aaacagcccc ggatgtttac ggataaaact
    85921 gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta ttcagagact caatcgtttc
    85981 ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca actttccagg
    86041 gaacacccta ttttaacgtg ggaatagtag aggggaagta acagatctac cacaattttg
    86101 cataacagtt gcgaaaaacc gattacaatt ttttaattat tctggctgga ttaccgccca
    86161 cgacaacgtt gtccgggaca tcttttgtga caactgcacc tgaggctacc acgacgttat
    86221 caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc aatccagacg ttattaccga
    86281 tggtgacggg tttccccagt tcagcaccgc tattacgtgc tacagggtcg atgggatgtg
    86341 ttgccgtgta gatatgaacg cctggtgcca acatacagtt atcaccgatg cgaatagggc
    86401 agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt attaccgaga aaaatgttat
    86461 agccatagtc acagcgaaac gttggctcaa tataagcctc tgtcacctga ccgaatagat
    86521 cagcgagaat ttgctggcgt aatgtgtgct cttccgccag ggaatgattg tatcggtgaa
    86581 taagctgacg agcgcgcagg cgatcgcgag ataacgtctc atctgccgag cgatacaact
    86641 caccagcaat catcttttct ttttctgtgc tcattactga cctccggttg ctgaaaatgc
    86701 ggcaacacag taagacgaaa agtcagtgaa gagaacgttc ccattacatc tgtgtgataa
    86761 agatcacata gggtatgtct tcatggcgaa aaagtataaa attcttaata aacagccggt
    86821 tatagctccg aaagcgaatt atttattagc gaataaattt ccatactgag gaagggatct
    86881 tgtcgtacag tttattcatg gtcaattcgg cgaggcggtg atctgcggct gagtaaaata
    86941 ccgccagttc attatctgat aattcgtatt tatttttctc gataacacgc tccagcgtgt
    87001 caattgtctg gcaacgacgt aaacgcatta aataatcggt tttcgttaaa ggtttttcgg
    87061 acatacttct acctatggtt gtaataattc taacaagata aactcgcagg attctctttc
    87121 gtcgcattga caaaacaacg gaatagtcga ttacctgact tccgccattt ctgaagatcc
    87181 tgcatattaa taccataact actgaacaac ataaaggtgt catccagata ttcgtcgatc
    87241 tgctcaatga gcttattgtc ttcattatac ttaattttgt aattaagtgc gaaggtcgca
    87301 atatgctcaa tcagttcatt caactggagg ttgatcgccg aggttgggtc gtttacccag
    87361 ccatgattgc tttcttcaag gtttgcaagg cagtcatgat acagggtttc acagagaaac
    87421 ttaagctgtg cgatatcatg tcttttgggt gagtattcat ccataacgcg tccccttctt
    87481 agcggttgaa ctaacggaca cctttcggga tggaaaaaac ttactgacct ggacttgccc
    87541 tctcgtttgt tagcttaact ataagccact ctttgcaggt tttcatcgca ttattacgaa
    87601 aaattacaat taggaaggaa gcgggacagg actcactttt tcatactagc actaacccgc
    87661 agcaggtaaa agcagtttgc tcgaactatt cataacattt caggattatc gccagatgaa
    87721 attggtgaca ctaataccag gattgctctg aatatgacgt aataaccgag gaatgaataa
    87781 agaattaccg caaataatta agaatagcct ctaaatgatt atggattatt aaatctatta
    87841 gcgctactta atataaatta actaacaatc agtatttttt atgaatttta tccgtggtta
    87901 atactggttt tcgtatgaga tcctgagttg gtggttcaat tactccttaa tgttcgtagg
    87961 ttatgcataa aaaaggccgc ttacgcggcc ttagtgatta cacgttgtat caatgatgat
    88021 cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg cgaaccacca
    88081 caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc ccgcccatta
    88141 caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata accagcggca
    88201 taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc atccgcaccg
    88261 catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct ttggcgaatt
    88321 cgacgataag gatcgcgttc ttcgccgaca acccaatggt tgtgagcagg cctacctgga
    88381 agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg ataaccccca
    88441 gcggaacgac cagcataacg gagaacggaa tcgaccagct ctcgtacagc gccgccagac
    88501 acaggaacac gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg ccggagagac
    88561 gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggcagt ttgctcgcca
    88621 gttgttccat cagctccatt gcttcaccgg tacttttacc cggtgccgcc tggcctaaga
    88681 tttccatgga tggcaggccg ttgtaacgtt ccagacgcgg cgaaccgtac tcccaacgag
    88741 aagaggagaa cgccgagaat ggcaccatct gaccatcagc agcacgaaca taccagtcgc
    88801 cgatatcatc cggcagcata cggtatttcg cttctgacat gacataaact ttcttcacac
    88861 gaccgcggtc gataaagtcg ttcacatagc tgccgcccca tgcagcgccc agagtggtgt
    88921 taatgtcgtt gatagaaaca cccagcgcct gcgctttttc ctggtcgata tcaatcttaa
    88981 actgcggggt atcttccaga ccgtttggac gtacgctggt caacatatca gggtgcttcg
    89041 ctgcttctgc aagcaactgg ttacgcgcct gagtcagttt ttcgtgacca aggccagcct
    89101 ggtcaatcag ctcaaagtca aagccggttg cagtacccag ttccacgatt gcgggcaggt
    89161 taaaggcgaa aaccatcgca tctttgattt gcgagaaagc gcgtgttgca cgcatggtaa
    89221 tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag gaaacgaacg
    89281 caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg aacaccgact
    89341 caacgttgtt cttttctttg gtcagatagt aatgcgttac ctcattgagc actttctgtg
    89401 tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaacacg ccctggtcct
    89461 catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg accacgatga
    89521 tcagatacag caccaggtaa cgccccgtac tgcgcagaat accgcctacg ctgtcggtgt
    89581 agtggtgcgt gctcttctcg aacatgcggt taaaccagcc gaagaagcct tttttacctt
    89641 ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagct ggagtcagga
    89701 tcaacgccac cagtaccgac agcgccattg ctgaaacaat ggtaatagag aactgacgat
    89761 agatagcacc agtagaaccg ccaaagaagg ccatcggtac gaataccgcc gacagtacca
    89821 tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta gcttcttttg
    89881 gcggcaaacc ttcttccgcc ataacacgct caacgttttc taccacaacg atggcgtcat
    89941 ccaccaacag gccgatggcg agcaccatcc cgaacattgt tagcgtgttt atcgagaagc
    90001 caaaggcggc aaggacggca aaggtcccga gcaataccac cggtacggca atggtcggaa
    90061 tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg aggatgatcg
    90121 cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggc gtggtgtcgt
    90181 acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc agttcagcac
    90241 ggattgccgc agcggtatcc agcgcgtttg caccggtcgc cagcttgatc cccagaccgg
    90301 aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc agctcaatct
    90361 tcgccacatc acgcagcagt acgcgggaac catcctgatt cactttcagc aggattttgc
    90421 cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta agctgttggc
    90481 ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc tgcgctttga
    90541 tggcggtaat gacatcaacc ggcgttagct ggaatttgtt cagctcattc gggttcatcc
    90601 agatacgcat cgcgtactgt gaaccgaaca actgaacatc acccacgccc gacgtacggc
    90661 tgatggcatc tttcatattc gccgccacgt agtcggagat atcctcctgc gtcatggtgc
    90721 catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc tcaacgctca
    90781 ccccttgctg ctgaacttct tgcggcagca acggcatcgc cagctgcagt ttgttctgca
    90841 cctgaacctg cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg atctgcacgg
    90901 tacccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg ttcatattct
    90961 gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg gggtaggagg
    91021 cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc agtttgagga
    91081 tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa atcgggcgat
    91141 cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt ggactgttca
    91201 ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg tgcttttacc
    91261 tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc tgctttcaga
    91321 ccttctgtca ccagccactt atcgccaata gcctggcttg caacgatcgg acgggtttcc
    91381 actttgtcat ccgcgccaac taccagtacg gtggcatcgc cacgcggcgt acgggttacg
    91441 ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg tgcgcgcacg
    91501 aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag ggtgatagac
    91561 ccagtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg cgggaactta
    91621 atgccgtcac tggtgatcag tgacactttg gctttgccgt tctcttgttt cagcgtgcca
    91681 ttcgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac atcaacgtag
    91741 atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg taccaatgcg
    91801 ccttccgtca cgttcgactt accaatgcga ccgctaatcg gagaggtgac tttggtgtaa
    91861 gccagattga tccgcgcagt ttcaacggca gctttcgccg cagttaccgc agcattcgcc
    91921 tgttgcgcat cagccagagc ctgatcgtac tcttgcttac tgatgtactg agtaccgagc
    91981 agtttctgat aacgattcac cgtcaattgc gcgatattgg ctgcagcctg ggctttcgcc
    92041 agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat ctgatagaga
    92101 gagacacctg cttcgatgtc gctaccttct ttgaaattac gcttcaggat aatcccgcta
    92161 acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag ctcggttgtg
    92221 atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg ctggccacct
    92281 tgttgggcct gtttgtcgtc acatcctgtt agggctaagc tgcctgagag catcagaacg
    92341 accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt gtccgatttc
    92401 aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta tcgtcgtgct
    92461 atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac gaacatatgg
    92521 cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat gtggctctac
    92581 gtcttttctc acagcagggg gtatcatcca cctcgctggg cgagattgca aaagcagctg
    92641 gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg ttcagtgaga
    92701 tctgggaact gtcagaatcc aatattggtg aactagagct tgagtatcag gcaaaattcc
    92761 ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa tccacggtga
    92821 cagaagaacg gcgtcgatta ttgatggaga ttatattcca caaatgcgaa tttgtcggag
    92881 aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat gaccgtatag
    92941 aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta atgacgcgtc
    93001 gcgcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg ctctttgccc
    93061 cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt tgccatctta ctggagatgt
    93121 atctcctgtg ccccacgctt cgtaatcctg ccactaacga ataaccctga atctgactcc
    93181 aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg tgatattctt
    93241 gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat catgactatg
    93301 ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc ttttgttttt
    93361 gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga tctgccgaca
    93421 aaagcggacc tgcaggcgca acttgactca ctaaataaac aaaaagatct ttctgctcag
    93481 gacaaactgg tgcagcagga tctgacagat acattagcca ccctcgataa aatcgatcgc
    93541 ataaaagaag agacagttca gctacggcaa aaagtcgctg aagcgccgga aaaaatgcgc
    93601 caggcgaccg cggcgttaac agcacttagc gatgtcgata acgacgaaga aacgcgcaaa
    93661 attctgagca cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc gctggacgat
    93721 ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc gttacagacg
    93781 cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca acaaattcgc
    93841 agtcgtctgg atgggactga tgtcggcgag acagccttac gtcccagcca gaaagtgtta
    93901 atgcaggccc agcaggcgtt gctgaatgcg gagattgacc agcagcgtaa aagcctggaa
    93961 gggaacaccg tcttgcagga taccttgcaa aagcaacgtg attacgtgac ggcgaacagc
    94021 gctcgtctgg agcaccagtt acaactgttg caagaagcgg taaacagcaa gcgcctgact
    94081 ttaaccgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg tattcaggct
    94141 aatccgctgg tgaagcagga actggaaatt aaccagcagt taagtcagcg tctgattacc
    94201 gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa ctggctggag
    94261 cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa gggcagcctg
    94321 ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga tgaactggaa
    94381 aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa ccagcagcgt
    94441 gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca caccaacgaa
    94501 gtcaacagcg aagttcacga tgcgttattg caagtggttg atatgcgtcg cgaattgctg
    94561 gatcaactca acaaacagtt gggtaaccag ctgatgatgg ccattaacct gcaaatcaac
    94621 cagcagcagt taatgagtgt gtcgaaaaac ctgaaatcca tcctgactca gcaaatcttt
    94681 tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc gcaaagcctg
    94741 aaagatgaat ttaagtcgat gaaaatcacg gtgaactggc aaaaagcctg gcccgccgtt
    94801 tttatcgctt tcctcgctgg tttgccgctg ctgttgattg ccgggctgat ccactggcgt
    94861 ctgggctggc tgaaagcgta tcaacaaaaa ctggcttccg ctgtgggttc cctgcgtaac
    94921 gacagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgtgc gctgccggtg
    94981 tgcctgatta ttctcgcggt tggcctgatt ctgttgacca tgcagctcaa catcagcgaa
    95041 ctgctatggt cgttcagcaa aaaactggcg atattctggc tggtgtttgg cctgtgctgg
    95101 aaggtactgg agaaaaacgg cgttgccgta cgtcacttcg gcatgccgga acagcagacc
    95161 agccactggc gtcggcaaat tgtccgcatc agtctcgcat tgctgcctat ccatttctgg
    95221 tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg gcaagcgatg
    95281 attttcttca acctgctgct gattgccttc ctggtatggc cgatgtgccg cgaaagctgg
    95341 cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtgctgtc gataatcccg
    95401 attgcgctga tggtgctgac tgctacaggc tacttctaca ctacgctgcg tctggcagga
    95461 cgctggattg aaaccgttta tctggtgatc atctggaacc tgctgtacca gacggtactg
    95521 cgtggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc gcgtcggcag
    95581 aatctggtga aagagggcgc agaaggtgcc gaaccgccgg aagaacccac cattgcactg
    95641 gaacaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc gctgttcggt
    95701 gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatct cgacagcatt
    95761 acgctctggc attacaacgg tactgaagct ggtgctgcgg tggtgaaaaa cgtcaccatg
    95821 ggcagcctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat tcgcaacctg
    95881 cctggtttgc tggaagtgct ggtgctctcg cgactgaata tgcgccaggg cgcgtcgtat
    95941 gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac ggtgttcgga
    96001 tcgctgggcg tctcttggga taaactccag tggctggccg cagcattatc cgtaggtctt
    96061 ggttttggct tacaggagat cttcggtaac ttcgtctccg gtttgatcat tctgttcgaa
    96121 cgtccggtgc gtattggcga tacggtaacc attggtagct tctcggggac ggtaagtaag
    96181 atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat catcccgaac
    96241 aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacactac tacgcgtctg
    96301 gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa agtgttactg
    96361 aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt cttctttacg
    96421 gcatttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga actgcgtgac
    96481 cgtagtcgta ctgtcgatga gctgaaccgt actatcgatc agctgtgccg tgaaaacgac
    96541 atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg cgatgaggtg
    96601 acggaagtaa aacgcgacta caaaggcgat gacccgacgc cagcggtagg gtaaaaacga
    96661 aagggggcaa catttagttg ccccgagatt gctaacaaag tgcgcgttgt tcatgccgga
    96721 tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatata ttgcaggagc
    96781 ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccgc aaccttaggg
    96841 gacatttagc gaccccattt atttctcact tttccgcctc atcatcgcgc gttaatttct
    96901 tttcataatc acgctttaca atatccagcg cgcgcagcac ggtactggca gggatctgat
    96961 tttcctccag cagcacaatc aaatcgacag ccagtttgac atcgtcaggg gcattttcca
    97021 gtgacatatt ctctccattg ctagcgggtt aaacgcgcta acctgttttc gattttttcc
    97081 agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg atgcagcgtt
    97141 tgctgttcca cgagatcggt cacccgcgcc agacgggctc tgcgttcggc aaccatctca
    97201 cgtagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg ggcaattttc
    97261 ggtggtgcac tgtcccactc gcgcaatgac caggcggagg cttcacgcgc gatggcttcc
    97321 agttgtgccg ccagatgttc cgccagccag gccacttgcg gcagctgttg ctgctcaact
    97381 gcatgacgaa gcgcagccag attatcgccc gcctcgtcga gacaagcctg tagtgttgtc
    97441 gcacgagtct gaaaaagatg cctgtcgaaa cgagcactta gcgtggcgaa ctgtgacacc
    97501 ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag cagcagggcg
    97561 gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag ccactctgta
    97621 agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg ctggcggtag
    97681 tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt atcctgctgg
    97741 cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg taccgctcat
    97801 ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc ggcgtcaaac
    97861 cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc gtccagatgc
    97921 catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttctat atgtggcgaa
    97981 ttccggtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca gttgcaatta
    98041 ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt caaaggttaa
    98101 acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa caagcaggca
    98161 tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca tcaaaagcat
    98221 tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac tggaagaccc
    98281 gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg cgggcattac
    98341 caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc gctccggtag ctctgggtct
    98401 gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa ccatcagtga
    98461 aacttacgac ctggaatacg gcaccgatca gctggagatc cacgttgatg ccatcaaacc
    98521 gggcgacaaa gttctggtgg tggacgacct gctggcaacc ggcggcacta tcgaagcgac
    98581 cgttaaactg atccgtcgtc tgggtggtga agtggctgac gctgcgttca ttatcaacct
    98641 gttcgatctc ggcggcgaac agcgtctcga aaaacagggc attaccagct acagccttgt
    98701 cccgttcccg ggccattaat tatcgccagt cttgtgctgc ccacgctacg gacagcacaa
    98761 gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc ttcgtgaatc
    98821 caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg aaaatggcgc
    98881 ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact ggcgaacggc
    98941 ttgtcgttag ggcgtattca tcatgcttat cttttttccg gcacccgtgg cgtcggaaaa
    99001 acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat taccgcgacg
    99061 ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt cgatctgatt
    99121 gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc gcgacctgct ggataacgtc
    99181 cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga tcgacgaagt gcatatgctg
    99241 tcgcgccaca gctttaacgc actgttaaaa acccttgaag agccgccgga gcacgttaag
    99301 tttctgctgg cgacgaccga tccacagaaa ttgccggtga cgattttgtc acgctgtctg
    99361 caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcagcttga gcacatcctc
    99421 aacgaagaac atatcgctca cgagccgcgg gcgctgcaat tgctggcacg cgccgctgaa
    99481 ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagcgg tgacggccag
    99541 gtttcaaccc aggcggtcag tgcgatgctg ggtacgcttg acgacgatca ggcgctgtcg
    99601 ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat taatgaagcc
    99661 gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggcct gttgcatcgt
    99721 attgcgatgg tacaactttc gcctgctgca cttggcaacg acatggccgc catcgagctg
    99781 cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta ctatcagacg
    99841 ctgttgattg gtcgcaaaga attaccgtat gcgccggacc gtcgcatggg cgttgagatg
    99901 acgctgctgc gcgcgctggc attccatccg cgtatgccgc tgcctgagcc agaagtgcca
    99961 cgacagtcct ttgcacccgt cgcgccaacg gcagtaatga cgccaaccca ggtgccgccg
   100021 caaccgcaat cagcgccgca gcaggcaccg actgtaccgc tcccggaaac caccagccag
   100081 gtgctggcgg cgcgccagca gttgcagcgc gtgcagggag caaccaaagc aaaaaagagt
   100141 gaaccggcag ccgctacccg cgcgcggccg gtgaataacg ctgcgctgga aagactggct
   100201 tcggtcaccg atcgcgttca ggcgcgtccg gtgccatcgg cgctggaaaa agcgccagcc
   100261 aaaaaagaag cgtatcgctg gaaggcgacc actccggtga tgcagcaaaa agaagtggtc
   100321 gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa aaacgccgga actggcggcg
   100381 aagctagcgg cagaagccat tgagcgcgac ccgtgggcgg cacaggtgag ccaactttcg
   100441 ctaccaaaac tggtcgaaca ggtggcgtta aatgcctgga aagaggagag cgacaacgca
   100501 gtatgtctgc atttgcgctc ctctcagcgg catttgaaca accgcggtgc acagcaaaaa
   100561 ctggctgaag cgttgagcat gttaaaaggt tcaacggttg aactgactat cgttgaagat
   100621 gataatcccg cggtgcgtac gccgctggag tggcgtcagg cgatatacga agaaaaactt
   100681 gcgcaggcgc gcgagtccat tattgcggat aataatattc agaccctgcg tcggttcttc
   100741 gatgcggagc tggatgaaga aagtatccgc cccatttgat cgtaagcaca gcttacgttc
   100801 gtcatcctta acgtgattga gagagaaacc tatgtttggt aaaggcggtc tgggtaacct
   100861 gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa atgcaggaag agatcgcgca
   100921 gctggaagtc accggcgaat ctggcgcagg tctggtaaaa gtgaccatca acggtgcaca
   100981 caactgccgt cgcgtagaga tcgacccgag cctgctggaa gacgacaaag agatgctgga
   101041 agacctggtg gctgcagcat tcaacgacgc agcacgtcgt attgaagaaa cgcagaaaga
   101101 aaaaatggcc tctgtatcct ccggaatgca gctgccgcct ggctttaaga tgccgttctg
   101161 atgcaaacca gcccgctgtt aacacagctt atggaagcac tgcgctgtct gccgggcgtt
   101221 ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc agcgcgatcg tagcggcggg
   101281 atgcgtctgg cgcaggcgct cacccgggcg atgtcggaaa tcggccactg cgccgattgc
   101341 cgcactttca ccgaacagga agtctgtaac atctgttcga atccgcgtcg tcaggaaaac
   101401 ggtcaaatct gcgtggtgga gagtccggcg gacatctacg ccattgagca gacggggcag
   101461 ttttcaggtc gttattttgt gttgatgggg catctgtcac cgctggacgg catcggtccg
   101521 gatgatatcg ggcttgatcg tctggaacag cgtctggcag aggaaaaaat cactgaagtg
   101581 atcctcgcca ccaaccccac ggttgaaggt gaagctaccg ctaactacat tgccgagctt
   101641 tgcgcgcaat atgacgtgga agccagccga atcgctcatg gcgttccggt tggcggcgag
   101701 ctggaaatgg tcgacggcac cacgttgtca cactcccttg ccgggcgtca taagattcgt
   101761 ttttaagcaa acgagagcag gatcacctgc tctcgcttga aattattctc ccttgtcccc
   101821 atctctccca catcctgttt ttaaccttaa aatggcatta ttgaggtaga cctacatgaa
   101881 aggacaagaa actcgtggtt ttcagtcaga agtgaaacag cttctgcacc tgatgatcca
   101941 ttctatctat tccaataaag aaatcttcct gcgtgagctt atctctaacg cctccgatgc
   102001 ggcggacaag ctgcgtttcc gtgcgctctc taacccggac ctgtacgaag gtgatggcga
   102061 actacgcgtt cgtgtctctt tcgataaaga caagcgtacg ctgaccatct ccgataacgg
   102121 cgtggggatg acccgcgacg aagtgattga ccatctgggg actatcgcta aatccggtac
   102181 caaatcattc ctcgaatccc tgggttctga ccaggcgaaa gacagccagc tgatcggtca
   102241 gtttggtgtt ggtttctact ctgcgtttat cgtggccgac aaagtgaccg tgcgtactcg
   102301 cgcggcaggc gaaaaaccag aaaatggcgt cttctgggaa tcggctggcg aaggtgaata
   102361 caccgttgcc gacatcacca aagaagatcg tggtactgaa atcaccctgc atctgcgtga
   102421 aggcgaagac gagttcctcg atgactggcg cgtgcgttcc atcatcagca aatactccga
   102481 ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag aaagacggcg aaaccgttat
   102541 ctcctgggag aaaatcaaca aagcgcaggc gctgtggact cgtaacaagt cggaaatcac
   102601 cgatgaagag tacaaagagt tctacaaaca catcgcccac gactttaatg atccgctgac
   102661 ctggagccac aaccgtgttg aaggtaagca ggagtacacc agcctgctgt acatcccgtc
   102721 ccaggctccg tgggatatgt ggaaccgcga tcataaacac ggcctgaaac tgtatgttca
   102781 gcgtgtgttc atcatggacg acgcagaaca gttcatgccg aactatctgc gcttcgtgcg
   102841 tggtctgatt gactccagcg atctgccgct gaacgtttcc cgtgaaatcc tccaggacag
   102901 cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt gtgctgcaaa tgctggaaaa
   102961 actggcgaaa gacgacgcgg aaaaatacca gaccttctgg caacagtttg gcctggtact
   103021 gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg atcgccaaac tgctgcgttt
   103081 tgcttctacc cataccgatt cttctgcgca gaccgtatct ctggaagact acgtttcccg
   103141 catgaaagaa gggcaggaga aaatctacta catcaccgca gacagctatg cggcagcgaa
   103201 gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc gaagttctgc tgctttccga
   103261 ccgcatcgat gagtggatga tgaactatct gactgagttc gacggtaaac cgttccagtc
   103321 ggtgtctaaa gttgacgagt cgcttgaaaa actggctgac gaagttgatg agagcgcgaa
   103381 agaagcggag aaagcactga ctccgttcat cgaccgtgtg aaagccctgc tcggcgagcg
   103441 cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg ccagcgatcg tttcgaccga
   103501 cgcggacgaa atgagcactc agatggcgaa actgttcgct gcggcgggcc agaaagtgcc
   103561 agaagtgaaa tacatcttcg aactgaaccc ggatcacgta ctggtgaaac gtgcggcaga
   103621 tactgaagat gaagcgaagt tcagcgagtg ggtagaactg ctgctggatc aggcgctgct
   103681 ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt cgtcgtatga accagctgct
   103741 ggtttcctga tgtaatgccg gatgaccttc gtgtcatccg gcatttttct tttcatcatc
   103801 tgcactttcc gcaaattatc tcgccattaa ccgtttcagc cccaggtgcc tttcttgagg
   103861 caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aattcgacac attttaaggg
   103921 gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag ggactcaggc
   103981 tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata tgctgcgtgc
   104041 tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa gacattatgg atgctggcaa
   104101 actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc aggaagactg
   104161 ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag acgcgatgaa
   104221 agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg aactgatcgt
   104281 tgaccgtatc gtcggtcgcc gcgttcatgc gccgtctggt cgtgtttatc acgttaaatt
   104341 caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagaactga ctacccgtaa
   104401 agatgatcag gaagagaccg tacgtaaacg tctggttgaa taccatcaga tgacagcacc
   104461 gctgatcggc tactactcca aagaagcaga agcgggtaat accaaatacg cgaaagttga
   104521 cggcaccaag ccggttgctg aagttcgcgc tgatctggaa aaaatcctcg gctaattcga
   104581 aagcgcgcac ggacagtccc ctcgccccct cggggagagg gttagggtga ggggaacagg
   104641 cccgcacaag caaacttatc agcaatctca ggccggatat tcattcggcc ttttacaaaa
   104701 acctcaatct gcgctattcc tttttctgat ttgacctctc acagcaatta gttcttcttc
   104761 ctcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaga
   104821 ctaaaaccgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg
   104881 taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt
   104941 tatggtggcc attgctgcgc ggcgtgattt tgccgctgcg ctcgccgcgt gtggcgaagc
   105001 tgtatgcctc tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac
   105061 agcaggcgct ggcacaacgt ttaccggaga tgcccgtagc gctgggaatg agctacggct
   105121 cgccatcact ggaaagcgcc gtagatgaac tcctggcaga gcatgtagat catattgtgg
   105181 tgctgccgct ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg gatgaactgg
   105241 cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttatacgt gattacgccg
   105301 ataaccacga ttacattaat gcactggcga acagcgtacg cgcttctttt gccaaacatg
   105361 gcgaaccgga tctgctactg ctctcttatc atggcattcc ccagcgttat gcagatgaag
   105421 gcgatgatta cccgcaacgt tgccgcacaa cgactcgtga actggcttcc gcattgggga
   105481 tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggtcgggaa ccctggctga
   105541 tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtaggt catattcagg
   105601 tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa
   105661 accgtgaggt cttcctcggt gccggcggga aaaaatatga atatattccg gcgcttaatg
   105721 ccacgccgga acatatcgaa atgatggcta atcttgttgc cgcgtatcgc taaagctgag
   105781 cggtaaagaa ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc atccgtgaat
   105841 aatgcaaaaa ggcgtgcagc gtgcctgggt agagtttgaa ctcacagggc tgctgatgcg
   105901 ccgctaacgt ctggtaaagc agacggctgt catccagcag cggatcgaac tccgccccgg
   105961 caataaaaca gggcggaact tcgcgagtga gatcattatt aaacagacag taatacggcg
   106021 actcgcggtc cgcgtcgttg cttaaatatg cctcttcgta catctgcaaa tcctgttgcg
   106081 ttaagccatc ccagacaccg cccaacagac gacgagtcac ggaatcccgt aatccgtaaa
   106141 gcccatacca cagcaaaaca cccgcaattt taccgcaatc gatctgttta tcacgcaacc
   106201 acaacgcact ggcgagcgcc agcatggcac ctgcggaatc accggcaaag ccaatgcggg
   106261 acatattgat ttgataatcc tccgcctgct ggtggaaata acaacaagca gccacaattt
   106321 cctctatcgc ttgcggaaaa cgcgcttcag gtgaaagggt gtaatcaata ccaatcaccg
   106381 tacattggct gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg agattgccga
   106441 gaataaaacc gccgccatgc aaataaaata gcgtcgccgg gctatctggc tgcggacaaa
   106501 agagacgtgt ttccacctgc ccatattttg ttggaaccat gtaagctctg gttgccattt
   106561 ctggagcgcc cgcattccag aatcggcgct caagcgtgta atactgtcgt tgctcagcaa
   106621 tcgttcccgt tgcgggccag ggcggtaaat ccggctgaag agtattcaca acggtcttca
   106681 tttcagcaga aataaggtcc agaacgggta atttgttttc cggcttcata caaaaactcc
   106741 tttcaaatta cgtcattgta aggaaccact gccatgaaaa tgcgatcccg cctgctgata
   106801 ttgaaactgg ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg ccgctcagtt
   106861 aaccacccgt aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa cattacttcc
   106921 ctgtaaatgc acgcgatccg ctgcttcagc aattccagcc agaaaacgaa accagcgccg
   106981 cctgggtagt gggtatcgat caaacgctgg tcgatattga agcgaaagtg gatgatgaat
   107041 tcattgagcg ttatggatta agcgccgggc attcactggt gattgaggat gacgtagccg
   107101 aagcgcttta tcaggaacta aaacagaaaa acctgattac ccatcagttt gcgggtggca
   107161 ccattggtaa caccatgcac aactactcgg tgctcgcgga cgaccgttcg gtgctgctgg
   107221 gcgtcatgtg cagcaatatt gaaattggca gttatgccta tcgttacctg tgtaacactt
   107281 ccagccgtac cgatcttaac tatctacaag gcgtggatgg cccgattggt cgttgcttta
   107341 cgctgattgg cgagtccggg gaacgtacct ttgctatcag ccctggccac atgaaccagc
   107401 tgcgggctga aagtattccg gaagatgtga ttgccggagc ctcggctctg gttctcacct
   107461 cttatctggt gcgttgcaag ccgggtgaac ccatgccgga agcaaccatg aaagccattg
   107521 agtacgcgaa gaaatataac gtaccggtgg tgctgacgct gggcaccaag tttgtcattg
   107581 ccgagaatcc gcagtggtgg cagcaattcc tcaaagacca cgtctctatc cttgcgatga
   107641 acgaagatga agccgaagcg ttgaccggag aaagcgatcc gttgttggca tctgacaagg
   107701 cgctggactg ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg tatatggcgg
   107761 gctttaccga agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg ggcgctatag
   107821 cggaattcaa ccagtatgag tttagccgcg ccatgcgcca caaggattgc cagaatccgc
   107881 tgcgtgtata ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc atgaacacta
   107941 acggagcggg ggatggcgca ctggcagcgt tgctgcatga cattaccgcc aacagctacc
   108001 accgtagcaa cgtaccaaac tccagcaaac ataaattcac ctggttaact tattcatcgt
   108061 tagcccaggt gtgtaaatat gctaaccgtg tgagctatca ggtactgaac cagcattcac
   108121 ctcgtttaac gcgcggcttg ccggagcgtg aagacagcct ggaagagtct tactgggatc
   108181 gttaagttat cgtcggttcg taggccagat aaggcgttca cgccgcatct ggcatttggc
   108241 tctcgatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacatatttt tcatatttta
   108301 catccggcaa ccaccgttta ccccgtcacc acttcacccg ccggtggcgt ttccagcagt
   108361 tccagcatag tacgggcaat ttcacgttca cccatcacta cctgattcgc gccacgttcg
   108421 gtaatatacg ccacttcatc gtcataatgg gcgcgggcaa taatctcaat atccggattt
   108481 ttcgcgcggg cagatgccac aatctcgcct gcttcataac cgttgggaat cgtcaggatc
   108541 agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc tgcattgccc
   108601 aatactgcgc ggaccccgcg ctctcgcagc tcatcaacac gggttcgcga cgtctcaatc
   108661 accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc tacacgaccg
   108721 taacccacca atagcgcatg gttgcaaata tccactggga tctgcttctc ttcttcgatt
   108781 gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt ctccagtagt
   108841 gcgaacagta ctgggttgag cataatcgac aggatcgccc ctgccagtac caggttttgt
   108901 ccggcctgcg gcagtaaatt caatgccatt cccagtcccg ccaggataaa cgcgaactca
   108961 ccaatctgcg ccaggctggc ggcgatggtt aatgccgtgc gctgggagtg accaaacaga
   109021 cgcacaagga gcagggccgc gaccgattta ccaaacagaa taatcgccag cgtcgccagc
   109081 actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac ggagacaaaa
   109141 aacagcaccg caaacgcgtc gcgcaatggc aacgtatcgt gggcggcacg gtgactcagt
   109201 tcagactcgt tcagtaccat cccggcaaag aacgcaccga gtgcaaagga gacatcaaac
   109261 agctctaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag ggtaaacagc
   109321 tcgcgagaac cggttgccgc gctgcgtgcc ataatccacg gcaccagacg gcgacctacc
   109381 agcatcataa tggcgataaa tgcgatcacc ttgccgatgg tgatccccat atcgactgca
   109441 agagtggcaa agcccacatc gccctgttcc atcattcctg ccactgcggg cagcaacacc
   109501 agcgtcagaa ccatcaccag gtcttccaca atcaaccaac cgatggcgat ttgcccacgc
   109561 tgactgtcta ttaattgccg ttcttcaagt gcgcgcagta acaccacggt actggcggtg
   109621 gaaagacata aaccgaacac gataccggtc attaacgacc agcccagcac ggcggagagc
   109681 gccataccca gcagcgtcgc cacggctatc tgggcgatcg cgccgggaat ggcgatggac
   109741 tttaccgcca tcaaatcctt gagcgaaaaa tgcaaaccga cgccgaacat cagcaaaata
   109801 acgcccagtt cagccaattc cggcgcaagc ttggtatcgg caacaaagcc cggagtgaat
   109861 ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg tagtttattg
   109921 gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt gataagcggg
   109981 gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt ggccgggaaa
   110041 accaaaatta caggtaatag tttatgacaa tttcattgat gatgtttatg aataattgtt
   110101 gaattttgca gaaaaatgga attagctgca aaaaaagcac ggatcggaaa acagaaggcg
   110161 gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt ttgcctttgg
   110221 ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa catccccaat
   110281 agtggcagga aagcacagat tttatagact aactcgatgc tggtgtgatc ggcgataagc
   110341 cccagaactg ccgcacccag acctcccatg ccgaaagcaa aaccgaaaaa gagtccagaa
   110401 accataccga tacgtcccgg aagcagctcc tgagcgtaaa ccagaatggc agagaatgcc
   110461 gaagcgagga taaatccaat aatcaccgtt aaaacccccg tccagtgcag gctggcgtag
   110521 ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc cccaaatcac atatttccgc
   110581 ccaattttat cccctacagg cccgccgatc accgtacctg ccgcaacggc aaacaggaag
   110641 gcaaacagat gaagctgagc attctggata gataatccga atttttgcat cagatagaag
   110701 gtgtaatagc tgctgatgct cgccatatag aaatatttcg agaaaatgag gattaacaga
   110761 atgctgaccg ccagtaccac tttgttgcgc ggcagtggat tgataatcgt cgctttgggt
   110821 tttcctttat tcattcggtg ctgtgccgag taccaacggc tgatttgtgc caacaccacg
   110881 atcgccagca gtgccgcaag cacaaaccag gcaacgttgc ctttgccata aggcgcgata
   110941 atcaccgccg ccagcaaggg tcccagggaa ctgccaaagt tgccgccgac ctgaaagata
   111001 gattgtgcca ggccatgccg cccgccggaa gccatacggg ccacgcgaga agattccgga
   111061 tgaaagaccg atgaaccggt accgaccagc gccgccgcca gcagaactgc gccaaaactg
   111121 cccgccagcg caagcagcac tagaccactt aaggtaaagc acatgccaat tggcaacgac
   111181 catggcattg gatatttatc ggtccagtag ccgaccactg gttgcagtag cgaagaggcg
   111241 agctggaagg tgagggttat catgccaatc tgcataaatg tcagagaaaa ttctgactga
   111301 agcagcggat aaatcgccag aatcagcgat tggatcatgt cgttcagcag atgtgagagg
   111361 ctgatagcac ctaaaatacc aaacgatgtt cgggccttgg tcgttgacgc agccgcgccc
   111421 gccacaggct gggtttgttc actcattgcc ataggaaagt cactttttca gggttgcgat
   111481 gtaaagaatg atcttatttg tgattattac ccgactaaca tacctgtatg cgtcgcctga
   111541 aggaagtctc aacgccgaat acagaatttc taatctggat gcagatttat cttcaccgga
   111601 cgcagacttg tctatgatgt cgcgtcatac tatttttcaa cacgttgaaa tcaggtcagg
   111661 gagagaagta tgaaattatt gcagcggggc gtggcgttag cactgttaac cacatttaca
   111721 ctggcgagtg aaactgctct ggcgtatgag caggataaaa cctacaaaat tacagttctg
   111781 cataccaatg atcatcatgg gcatttttgg cgcaatgaat atggcgaata tggtctggcg
   111841 gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg cggctgaagg cggtagcgtg
   111901 ctgctacttt ccggtggcga cattaacact ggcgtgcccg agtctgactt acaggatgcc
   111961 gaacctgatt ttcgcggtat gaatctggtg ggctatgacg cgatggcgat cggtaatcat
   112021 gaatttgata acccgctcac cgtattacgc cagcaggaaa agtgggccaa gttcccgtta
   112081 ctttctgcca atatctacca gaaaagtacc ggcgagcgcc tgtttaaacc atgggcgctg
   112141 tttaagcgtc aggatctgaa aattgccgtt attggtctga cgacggatga cacagcgaag
   112201 ttaggcaatc cagaaaactt caccgatatt gagttccgta agcccgccga tgaagcgaag
   112261 ctggtgattc aggagctgca acagacagaa aagccagaca ttattatcgc ggcgacccat
   112321 atggggcatt acgacaatgg tgagcacggc tctaacgcac cgggcgatgt ggagatggcg
   112381 cgcgcgctgc ctgccggatc gctggcgatg atcgtcggtg gtcactcgca agatccggtc
   112441 tgcatggcgg cagaaaacaa aaaacaggtc gattacgtgc cgggtacgcc atgcaaacca
   112501 gatcaacaaa acggcatctg gattgtgcag gcgcatgagt ggggcaaata cgtgggacgg
   112561 gctgattttg agtttcgtaa tggcgaaatg aaaatggtta actaccagct gattccggtg
   112621 aacctgaaga agaaagtgac ctgggaagac gggaaaagcg agcgcgtgct ttacactcct
   112681 gaaatcgctg aaaaccagca aatgatctcg ctgttatcac cgttccagaa caaaggcaaa
   112741 gcgtagctgg aagtgaaaat aggcgaaacc aatggtcgtc tggaaggcga tcgtgacaaa
   112801 gtgcgttttg tacagaccaa tatggggcgg ttgattctgg cagcccaaat ggatcgcact
   112861 ggtgccgact ttgcggtgat gagcggaggc ggaattcgtg attctatcga agcaggcgat
   112921 atcagctata aaaacgtgct gaaagtgcag ccattcggca atgtggtggt gtatgccgac
   112981 atgaccggta aagaggtgat tgattacctg accgccgtcg cgcagatgaa gccagattca
   113041 ggtgcctacc cgcaatttgc caacgttagc tttgtggcga aagacggcaa actgaacgac
   113101 cttaaaatca aaggcgaacc ggtcgatccg gcgaaaactt accgtatggc gacattaaac
   113161 ttcaatgcca ccggcggtga tggatatccg cgccttgata acaaaccggg ctatgtgaat
   113221 accggcttta ttgatgccga agtgctgaaa gcgtatatcc agaaaagctc gccgctggat
   113281 gtgagtgttt atgaaccgaa aggtgaggtg agctggcagt aatccgaaag tgccggatgt
   113341 ttgcatccgg cacaatgctt aatcgcggcg ggcgatatca gcaaatttgg catcgaggat
   113401 ctttgccaga tcgcctgccg ccagttcgat atccagtccg cgcttgccgc cggaaacata
   113461 aatagtggca aattcttgtg cgggggcgtc gataatcgtt ggcagacgtt ttttctgccc
   113521 cagtgggcta atccccccaa ccaggtatcc cgtcgaacgc tgcgcgacca tcggatcggc
   113581 catctcaact ttcttggcac ccagcgcttt tgctactttt ttaagatcca gttgacctgc
   113641 gaccggcgta acggccacgg caaggtgttt catatcaccg ttcactgcca ccagcagcgt
   113701 tttgtagacc tgatccggat tcaaacctaa ttttttgacg acttcatcgc caaaattggt
   113761 ttcagccgga tcgtgctcgt aggtatggat ttgaaacgaa atcttgtttt tttcgagtaa
   113821 tttaactgcg ggtgtcatgg taattttcct gccttaaaca aaaaaacgaa gcaagcatac
   113881 gcctgatggc ggtccaaaaa aaagagtcat cttgcctaag agtattggca ggatggtgag
   113941 attgagcgac aatcgagtta caccgtcgct taaagtgacg gcataataat aaaaaaatga
   114001 aattcctctt tgacgggcca atagcgatat tggccatttt tttagcgcaa catttgcggc
   114061 aaattccctt ctccatacag gtgtagtgca ccgaccgcga ccacatatcg ccccggcggc
   114121 atggcgcgta atttatcccg ccaggcgaga tttcgctgat gcatcagcac atcgtacagc
   114181 gactgactga acgtattggg cagcgttata tcattatttt gcggcggtgc attcagccac
   114241 cagctcatca tttgttgcag caaccgtgcg ttggtatgcc agtgggtcag cgtatcgtcc
   114301 agcagcgcca gtcctttgtc agggagctgg agcaacatgg caatctggtt ttcagcccct
   114361 tccagttcaa tcacgggttt atgttgttgc ttcgccgcct gcaatagctg gtaatcgata
   114421 ccgtattctg cccgcagccc cagtttttgt gcctgcgtcg cctgaagaac catcgcgatt
   114481 tgccacagcg gttgggtaga aaagagtgag ggagaaatgc ccatctcctg gctaatgtgc
   114541 tgcaggtttt ggagttgctc ctcgctaatg cgctcttcca gcgcctcgca ggcaggcaaa
   114601 ttagcaaaag gcgtatcgct ggtggaaaca tccgcctcga cgatcagcgc atcggcgttt
   114661 ttgagctttt tgagcaaacg ggtgggcagg ggagccatat cgtggctacc catatgaata
   114721 ctgccaatca gatgaaaatg gcgattgccg gggagggtga tatcgatggc aggccaggtg
   114781 taatgattac cgcgcagcgc ggcccaaagc gtttttaccc ggtacaacag atccatacga
   114841 cctccctttg tgaaatatca tgctagcgcg cggtgaggga tggcgcaaat ggagttggag
   114901 acttgtttaa tgtgtttgta tgattcagta tgttcttgca tcgctattca caaggaagca
   114961 acagttaaaa accatgaaac aggcaacaag aaaaccgacg acacccggag atattcttct
   115021 ctatgaatat ctggaaccgc tcgatttgaa aatcaatgag ttagcagagt tgctgcatgt
   115081 tcatcgtaat agcgtcagtg cactgatcaa taacaatcgt aaactcacta ctgagatggc
   115141 atttcgtctg gcgaaagttt ttgataccac agtcgatttt tggctaaacc tccaggcggc
   115201 ggttgatctt tgggaagttg aaaacaacat gcgcacccag gaagaattgg gacggattga
   115261 aacagtggct gaatatttgg cacgccgtga agagcgtgca aaaaaggtcg cgtaaggcca
   115321 aaacatcgct tcatctcgta ggccggataa ggcgttcacg ccgcatccgg catccgagca
   115381 ccattgcctg atgcgacgct tacgcgtctt atcaggccta caaacctgtg cctgaaccgt
   115441 aggccggata aggcgtttac gccgcatccg gcagtcatgc gtcgatgcca aatgcgccac
   115501 cctaaagcag cgcatccgca atgatgtact tattccttcg gtttaaaccg cagcaaccgg
   115561 ttggcgttac tcactacggt aatcgacgag agcgccattg ccgctccggc aactaccggg
   115621 ttaagcagtg ttccagtgaa cggccacaaa ataccggcgg cgaccggaat accgatactg
   115681 ttgtagataa acgcaccgag caggttctgc ttcatgttgt gcagcgttgc gcgggaaata
   115741 gcgagcgcat ccgcaacgcc catcaggcta tggcgcatca gggtaatcgc cgcggtttca
   115801 atggcaacat cactgccgcc acccatcgca atgccgacat ccgcctgagc cagcgctggc
   115861 gcgtcgttaa tgccgtcgcc caccattgcc acctgacgtc cttcactttg cagatgtttg
   115921 atcgcttcgg ctttaccgtc cggcagcacc ccggcgatca cctcatcaat ccctgcttct
   115981 ttggcgatcg cattggcggt ggttgggtta tccccggtca acatcaccag acgatatccc
   116041 gctttatgca ggcgttgcag cgccgccacg ctatcactac gcaacggatc gcgtactgcc
   116101 agcagggcta ccgctttccc gtcaaccgcc agcagcacag gcgttgcccc ttgcgatgcc
   116161 tgagcagtaa tctccgcttc gatagctttg gtaccaacct gttgctcatt taacagcgcc
   116221 tgattgccca gcaataacgc atgaccttca gcttcaccgc tcacgcccag cccgcgcaat
   116281 gtgcggaaac cgttgacctg cggtagctgc atatcacctg ctttatcgag gatcgctcgt
   116341 gccagcggat ggctggaacc ttgctccagt gccgccgcca gacgcaatgc ctgcgcttca
   116401 tcaacatcag caaatgtttt cactgcgaca acctgcggct tcccttcagt cagcgtcccg
   116461 gttttatcga acactacagt gtcgagtgta ctggcgcgtt gcagcgcgtc agcgtcccgc
   116521 accagcacgc caaactcagc cgcccgcccg acgccggaaa taatcgacat cggcgtcgcc
   116581 agccccagcg cacacggaca ggcaataatc agtaccgtgg tggcaatcac cagggtatag
   116641 acaatctgcg gtgccggacc aaagaaatac cagattgccg cactgacaag cgcaataacc
   116701 accactaccg gcacaaatac ggctgagatt ttatccgcca gctgaccgat ttctggcttg
   116761 ctgctctggg cctggcgcac catgcgaatg attcgtgaca gcgtagtatg gctgccaacc
   116821 gcactggcac gaaacagcac actgccgtcc tgtaccactg tcccggcatg gacgctatcg
   116881 ccttcgcctt tttgctgcgg gattggttcg cccgtcagca tcgcttcatc cagccatgct
   116941 tcgccctggg taatctcgcc atctaccggc acgcgatcgc cggtcgtcag gcgcagcaac
   117001 atacctggct gcacttctgc cagaggcacg cttttttcac cttcgtcagt aaccaggcgt
   117061 gccgtcggcg gggttaaatc gagtaacttt tccagcgcct tagaagaacg ctggcgtgcg
   117121 cgcgcttcca gcatatggcc gagattgatc agaccgataa tcatcgcgct ggcttcgtaa
   117181 taaagatgtc gcgcttccat cgggaaccac tgcggccaca ggttgacgct catcgaatag
   117241 agccacgcca cgccagtacc cagcgccacc agcgtatcca tcgtcgccgc accgttcagc
   117301 aggcttttcc atgcactgcg gtaaaaatgg ccgccggcga aaaccatcac tgccagggtt
   117361 atcaggccga taaccaacca caggctgcgg ttgtcagcgg tgaccatcat gttatcgccg
   117421 atcatccccc agaccatcac cgggataccc accgccagtg cgacaattgc ctgccagcgg
   117481 aagcgcttca tcgtagcgac ggcggtttct tgctggcgct cgcggcgttt agcgtcatct
   117541 tcaatcgctt ccgcgccgta gcccgctttt tccaccgcct gcactaaatc ttgtggggag
   117601 gcactgccca tcaccagcgc agtacgctcc gccaggttta cccgtgcctg agtgacgccc
   117661 ggtacgcttt gcagcgcatt ttgtacgcgg gtgacacagc tggcgcagct catgccgctc
   117721 agcagcaact gctggctgtc atcgtcatcg gcggtcgctg ccggaagcgc ctcagaaacc
   117781 gctgtcagtg cttccgacgg gattgatgac tccgccagcg gtttagcctt tgggtggctt
   117841 acagatgcgt cataacccgc ttgtttgatg gtttcgatca gctgttctgc actggcagtc
   117901 ccggtaacgt gcgcttcagt gatagacaca tccgcctgct caacatccgg acgctgttca
   117961 agactttctt tcacgcgttt aacgcagtga ccgcaggaca ggccgtccag ggtcaggtcg
   118021 atagtttgtg acataaaaca ctcctttaag acagttttga ctggctgtga taaaggttaa
   118081 accttccagc aaggggaagg tcaagcattt taacagtctg attaagaaag gattatttaa
   118141 tcagtgtcca cgtagtgtcc atttttgaag aaaaaaagcc cgcaatcctg acgttgtggg
   118201 cttttctttt tcctggtggt atcgtgctgc cagtgcaacg aaccgaaggg agggggtgtt
   118261 gatgttattt gcccgtcgta ccagccttaa agccacgctt gttaacgatg atggtacgcc
   118321 acggatcact gtcgaggtga ggaatgtggc gcctgatgat atcgggaagg tggcggaact
   118381 tgtcagaggt gtttttggta ctgcttccgg tgcctgccgt tcagactctg cggtcagggt
   118441 tgacgggcag gatgttaacg ccgggtggct tacgcgcctc tgaatgatgg taccctcact
   118501 ggtgagggtg gcaaaaaaaa gcccgcgcaa cgggcgggca atgctggatt ttctttggtt
   118561 cgtcataagg aaaacgggcg ccatatcggc ccaccccaac aaccggatag cgggggattt
   118621 ctcccccgct gcggtttctt acggtctgca ctgcaagaac gccgcaaaac gcgcacccca
   118681 gatatcaaag ctgaatccac ataacgaatc gtggaccatc cagatgacca gggttattgt
   118741 caggcataaa atgccgacaa tgatcgcttt ttgcgacata gcgcttgctc cttttacaaa
   118801 gaggcgctaa ccttccactt gctaggttgg atcggttagg gcctcgggtt aacgttaagt
   118861 tgactcgggg cctttccaca tccggccttc aggttttccc tccggccatc agccgaaagg
   118921 cacccgcgcg taatttaact gtttcttttt gttgcggcaa tatttcaggt gtcagttggg
   118981 gtgggctgta tcttgtctga tgcataaaaa aatgcccgcg cttcggcggg caaaaaacaa
   119041 gacttttcgt ttgtatatca tggataatgg acaccccttc ctgttgtcag gtggcaggta
   119101 cccgtggtta atctactgtt tttgtgcttt tgtctcaacc atgaattttt catggaaaaa
   119161 tcaggtctgc ttaatatgtg tcatgaaggt gcgtccatga taactgccaa aaaaatgccc
   119221 accttctggc gggcaaaaca gcaatgtgaa ttgtcagtca ttaatacgta ctggataccg
   119281 ttcctgttgg caggtggcgg gtacccttgg ttaatctact gtctttgtgc ttttggctca
   119341 accctgaagt tttcatggaa aatcagggtt gctttgtgtg aaccaggaag gtgaggcgat
   119401 ggtaaccgcc aaaaaaatgc ccgctttccg gcgggcaaaa caaggaagta tggatcatgt
   119461 attactgatt acg
//
LOCUS       DBBGLQ010000003       118444 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.003, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000003 DBBGLQ010000000
VERSION     DBBGLQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 118444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.003"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..328)
                     /locus_tag="ACIYSB_000262"
     CDS             complement(<1..328)
                     /locus_tag="ACIYSB_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5427390.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(325..3390)
                     /locus_tag="ACIYSB_000263"
     CDS             complement(325..3390)
                     /locus_tag="ACIYSB_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5427391.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQITALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATEKMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELMQALTLLDVKQQELQKQTALNDLKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVAQICGSGMAMFAPQ
                     YNIEINNDGMNGQIGPAALKVVYDLGKKAAADFMQQQSRDGGLFSGGGR"
     gene            complement(3362..3691)
                     /locus_tag="ACIYSB_000264"
     CDS             complement(3362..3691)
                     /locus_tag="ACIYSB_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGC5427392.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(3700..4086)
                     /locus_tag="ACIYSB_000265"
     CDS             complement(3700..4086)
                     /locus_tag="ACIYSB_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGC5427393.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLYASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(4147..4890)
                     /locus_tag="ACIYSB_000266"
     CDS             complement(4147..4890)
                     /locus_tag="ACIYSB_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5427394.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGRGDPFANPLQDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTISPVSASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            complement(4901..5302)
                     /locus_tag="ACIYSB_000267"
     CDS             complement(4901..5302)
                     /locus_tag="ACIYSB_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGC5427395.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(5299..5889)
                     /locus_tag="ACIYSB_000268"
     CDS             complement(5299..5889)
                     /locus_tag="ACIYSB_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5427396.1"
                     /translation="MKGLENAIRNLNSLDTRMVSQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRSGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRRLPEARFATGHDHQGRPRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(5901..6176)
                     /locus_tag="ACIYSB_000269"
     CDS             complement(5901..6176)
                     /locus_tag="ACIYSB_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGC5427397.1"
                     /translation="MMPPFQSVCSRMDSVTVRRMGRTACINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKTMIFIE"
     gene            complement(6326..6643)
                     /locus_tag="ACIYSB_000270"
                     /pseudo
     CDS             complement(6326..6643)
                     /locus_tag="ACIYSB_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
     gene            complement(6730..8757)
                     /locus_tag="ACIYSB_000271"
     CDS             complement(6730..8757)
                     /locus_tag="ACIYSB_000271"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGC5427398.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDNESEASWTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSGYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(8702..10210)
                     /locus_tag="ACIYSB_000272"
     CDS             complement(8702..10210)
                     /locus_tag="ACIYSB_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGC5427399.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSEVTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRALDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            complement(10210..10422)
                     /locus_tag="ACIYSB_000273"
     CDS             complement(10210..10422)
                     /locus_tag="ACIYSB_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427400.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(10419..12518)
                     /locus_tag="ACIYSB_000274"
     CDS             complement(10419..12518)
                     /locus_tag="ACIYSB_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGC5427401.1"
                     /translation="MTFASASAIRRDVSELLKPPTRMPVAEAVEKYMRVPLGGGSSLP
                     WDASLTPYIIEPMNCLSSRDYDAVVFVGPARTGKTVGLIDGWIVYSVVCEPADFLLIQ
                     MTEEKAREHSRKRLDRTFRVSKEVVRRMSPRTNDNNVHDKTFKAGNYLKIGWPSVNIM
                     SSSDYRFVALTDYDRWPDDIDGEGDGFSLASKRTTTFMSAGMTLVESSPGREISDSKW
                     RKTSPHEAPPTTGILSLYNRGDRRRWYWPCPDCGGYFQPSMETMTGYRDNPDPVKASK
                     SAHILCPHCGNRITSDMKRELNGRGVWLRDGEQIDMEGNVTGTPRNSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPHGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRGD
                     SDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            complement(12515..13018)
                     /locus_tag="ACIYSB_000275"
     CDS             complement(12515..13018)
                     /locus_tag="ACIYSB_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGC5427402.1"
                     /translation="MSNISNIGDVYHWSIAKIAEAFAMDRKVVKKRLLEANIPVAGTV
                     RGNPVYALRDVGPALFESKSVIASDIHDPSRMEPKERKDWFQSENERIKMETTLKQLV
                     PASDVHREMALMLKVVTQVLDTWPDKLERDRGWSASQIAEAQRAVDDIREALSTEMRE
                     RDDEVEL"
     gene            13385..13561
                     /locus_tag="ACIYSB_000276"
     CDS             13385..13561
                     /locus_tag="ACIYSB_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427403.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLSQKEFPVGLRSWLMQEYGD
                     DTAHNVSGSNTFIR"
     gene            complement(13703..13855)
                     /locus_tag="ACIYSB_000277"
     CDS             complement(13703..13855)
                     /locus_tag="ACIYSB_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427404.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSESRLNISLLMLEVEHLA
                     RGKGLR"
     gene            complement(13843..14310)
                     /locus_tag="ACIYSB_000278"
     CDS             complement(13843..14310)
                     /locus_tag="ACIYSB_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGC5427405.1"
                     /translation="MSRVAAIIYALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADVLDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCSVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(14307..14840)
                     /locus_tag="ACIYSB_000279"
     CDS             complement(14307..14840)
                     /locus_tag="ACIYSB_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGC5427406.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            15006..15341
                     /locus_tag="ACIYSB_000280"
     CDS             15006..15341
                     /locus_tag="ACIYSB_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427407.1"
                     /translation="MTEKVKSALLPLMVDNNEATSSILADPYGKLSHKTLDIIMSALT
                     PRMLQHLKQDINAWVNEELSPPCSWDRHYAWQQKMRIFNSLSLKFREPKQRPFPLESD
                     KKQPDPTGQ"
     gene            complement(15498..15770)
                     /locus_tag="ACIYSB_000281"
     CDS             complement(15498..15770)
                     /locus_tag="ACIYSB_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427408.1"
                     /translation="MRMDERYSNAAYGSAGLVAFFASLSLQDWGFIIGVAFSIILGVL
                     TYRLNKREQEKRTRILLEILERTNAGNVPATAHAVAEIAKKAPKEI"
     gene            complement(16186..16262)
                     /locus_tag="ACIYSB_000282"
     tRNA            complement(16186..16262)
                     /locus_tag="ACIYSB_000282"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16226..16228),aa:Lys,seq:ttt)
     gene            complement(16264..16339)
                     /locus_tag="ACIYSB_000283"
     tRNA            complement(16264..16339)
                     /locus_tag="ACIYSB_000283"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16304..16306),aa:Ile,seq:cat)
     gene            16938..18044
                     /locus_tag="ACIYSB_000284"
     CDS             16938..18044
                     /locus_tag="ACIYSB_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGC5427409.1"
                     /translation="MKNLTVALSAVAASVFLATSSNAAEIYNKDANKVDLYGKAVGLH
                     YFSSDKHQNGDQTYARLGFRGETQINNYITGFGQWEYNFQGNNPEGDGAQTGNKTRLA
                     YAGLKLADAGSLDYGRNYGVVYDALSYTDVFPEFGPTAAQKDNFFVGRVGGVATYRNT
                     DFFGLVDGLSFAIQYLGKNERKDKPDTSNGEGVGGSVSYEYEGFGFVAAYGAADRTKN
                     QKKAELGKGKKAEQWATGVKYDANNLYLAASYGETNNTRAISYGDNISGVANKTEDFT
                     VVAQYQFDFGLRPSLAYTHSKAKNLSSSTDISKKHDDGYIEKFVEVGASYYFNKNMST
                     YIDYKINMLDKNKFTERTGVSTDDIVAVGLVYQF"
     gene            complement(18223..18645)
                     /locus_tag="ACIYSB_000285"
     CDS             complement(18223..18645)
                     /locus_tag="ACIYSB_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGC5427410.1"
                     /translation="MRDIQMVLERWGAWVANNHEDVEWSGIAAGFKGLIPSKVKSRSQ
                     CSDDDAMIICGCMARLKKNNADMHDMLVDYYVYGMTFMKLAMKHGCSDTHIGKRLQKA
                     EGVVDGMLMMLDVRLEMDRYVSTNYSIRRKTASSRAGI"
     gene            complement(18645..19502)
                     /locus_tag="ACIYSB_000286"
     CDS             complement(18645..19502)
                     /locus_tag="ACIYSB_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5427411.1"
                     /translation="MRTDNNEHKVLFTIPTAAYSSALANIKPLPEQRRITGHKQTDAY
                     LWVLEVIRLNEPAHLDAAEAALVKIKISPKEAQERYSRYLLANGYEPFQVAFGIIGMD
                     NPAQVIRNARENIKKAASVRATFGSYEAALEDVEAERVIRSSQKFINDHLWGWTAAEK
                     KAGRIDGIRMNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFVYWDWLYSVRHTATKEQ
                     GDEYGYSEHHESVYDRERYLEKLLMTIKPVTRAEAVEVCRWFLASGKGEYMKDNGAAV
                     ILNLVGECE"
     gene            complement(19502..20470)
                     /locus_tag="ACIYSB_000287"
     CDS             complement(19502..20470)
                     /locus_tag="ACIYSB_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HGC5427412.1"
                     /translation="MKTADAAKGRWPEILEHFGLPPITGKNHFKGECPVCGARGKFRI
                     DDRDGAGTWICVCGSGDGMKLVTLTQAKPFNEICTEIDRLLGNDYQRVKIPVTSSATS
                     LRKRVLSKFSKLEALRGTSGAAYLNSRGIFSLPAEAIRFNARQRHNGSVFQSLYSLAT
                     DDKGELCYLHQTLLDGDKKADIGSSAKRLKSLQEDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYNVNTWATINSGFMKKFRVPAGVLHLIIFADRDENSATGLAAACEC
                     AHANLMAKNDLQRVSVYWPDHDDFNNMLMNGDQVRELVFHKKKAVA"
     gene            complement(20472..22130)
                     /locus_tag="ACIYSB_000288"
     CDS             complement(20472..22130)
                     /locus_tag="ACIYSB_000288"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGC5427413.1"
                     /translation="MQLTITPNFAQERALNMLRRDWKANDTFMVYSPTGSGKTGLAAF
                     IVAGFVSRGMRVLFCVPYTILIGQTANRFVQYGLPGDEIGYIWADHPNYDPDRKIQIA
                     SADTLIRRVFPENIDLLIIDEAHLRKKRILKDIERLRGKGVKVIGLSGTPFSPFLGKY
                     YDRLIKPTTIGELIQRGDLSKYEFYAPTKPDLKGVKTTSSLEYGRDYNETQLAEIMCG
                     STLVGDIVQNWLENGRDLPTIAFCVNVAHANYLTIQFNLAAVNAEVMTADTPVDERQT
                     IIHRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQALGRGLRTAPGKES
                     CLIFDHSGTVHRLGYPDSIEYDDLPGKSDGMEESTRRAVEERAEKLPHECPQCHYMKP
                     AGVYVCPKCGHKPLRGEDVDTDTSRKLNKLGKNQHQSTKAEKQAWWSQIKFYQRQRAS
                     LGRAVSDGWCAHTFREKFGEWPDGLSSFPMEISPEVSNYIRHKLIRFAKGRERTRRMT
                     KNSSEVLSLPLVVGGRYVPPEESEAWHIMQAKQQFQKNINSLSQ"
     gene            complement(22121..22723)
                     /locus_tag="ACIYSB_000289"
     CDS             complement(22121..22723)
                     /locus_tag="ACIYSB_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGC5427414.1"
                     /translation="MVNSFDGEKWLPVPVAPYGDAYLVSSAGRIRGVNRIAESEFFIR
                     QIRGVFLKGRVRRDGYKTVNLSYKRNRQTFAVHRLVALAFCSNPYNHPEVNHKDGNKL
                     NNHASNLEWVSHSENIQHSIHTGLNNSQGANNKQFKGLIVATNIATREEQTFCGKKAL
                     ISAGFDHGSVYAVIAGRTKSHRGHHFRRLPLNHGEAAQCS"
     gene            complement(22982..23275)
                     /locus_tag="ACIYSB_000290"
     CDS             complement(22982..23275)
                     /locus_tag="ACIYSB_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427415.1"
                     /translation="MSQNYVQTEMPSRYCQADEEWIQQQLQRLPPSLRRKVALKYAEV
                     YEITFDAEPVSFRKENRARHEANTRLRLFVRNQGRALQGYTTQPPLAGTQSRS"
     gene            complement(23238..23525)
                     /locus_tag="ACIYSB_000291"
     CDS             complement(23238..23525)
                     /locus_tag="ACIYSB_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5427416.1"
                     /translation="MKHKAIEKAIEILGSQQALAKRCGKAQSTVCDWLNCKNKISPEF
                     VPLLVAAVDGKIQAYEFRPDLPELFPHPSLARTGEVKGLYVPELRSDRDAI"
     gene            23645..24337
                     /locus_tag="ACIYSB_000292"
     CDS             23645..24337
                     /locus_tag="ACIYSB_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5427417.1"
                     /translation="MTFSERLDLAMRNAKFTQGRLAKEVGMAQSSVNQLLNKANGSRK
                     TVEIAKVLGVNPEWLASGVGPMEIVTSADSHQIRNISEDWVTDSYVVDVLDIRYSCGP
                     GSYNSDFPDIVRSIAIEPGYASRVFGGRPASAIKAINAHGDSMKGTIDPEDLVFVDVS
                     VRTFNGDGIYAFTYSGTSHIKRLQKIKDTLTVISDNPAYKDWAIEPEDFEQLHIDGKV
                     IVSWPMTLHRFA"
     gene            24480..24890
                     /locus_tag="ACIYSB_000293"
     CDS             24480..24890
                     /locus_tag="ACIYSB_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5427418.1"
                     /translation="MSSYMGLPTNQHDALENIQLLVGAGALLYSSNNPELNELASSIL
                     AVTQKYSLTASRLCKQNLAINSQNRIRTQREDCGLTTAELARLLDLDEEIIIQWESGE
                     YEPTISMLIPLANVLGCDPMLLLTDAYKQNQEEA"
     gene            24892..25083
                     /locus_tag="ACIYSB_000294"
     CDS             24892..25083
                     /locus_tag="ACIYSB_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427419.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDDECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            25456..25815
                     /locus_tag="ACIYSB_000295"
     CDS             25456..25815
                     /locus_tag="ACIYSB_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427420.1"
                     /translation="MAYEIIGKYRHRIVTTALQRMQRKTGGNLLIVNLPDGGLTTIEI
                     TEHFMTQLLLRFEGLTRVEFGRTDGETAILAAYRNAININQHTEYLTESGKLIVDNLL
                     KEVVDYEKEKYTSGGIN"
     gene            25815..26024
                     /locus_tag="ACIYSB_000296"
     CDS             25815..26024
                     /locus_tag="ACIYSB_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427421.1"
                     /translation="MSDLSPLIHEKVQLITTIENGQVINTRKVHNNGMIASMDTFLWI
                     AKKAGYQVIAPDLEEIGDINSNTNS"
     gene            26311..26829
                     /locus_tag="ACIYSB_000297"
     CDS             26311..26829
                     /locus_tag="ACIYSB_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427422.1"
                     /translation="MIGYSTAVRQIDNGNYDKRIPDGLDLIACIMEGIESGWFMPCIE
                     KQIILWRWLVVAVFIAEEQEKNGTVDVQNDKGGVDTAVVYTGKPGSISIYPWSERFAL
                     ANHIEAGAIEKYGPDVGQQMALRMYQDMVVTDEKCGFRLSAMGREGLNILHDRFIELI
                     QKDGMPDMPVMH"
     gene            26841..27587
                     /locus_tag="ACIYSB_000298"
     CDS             26841..27587
                     /locus_tag="ACIYSB_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGC5427423.1"
                     /translation="MNIVTINNKQLPAVEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMTDAKCRNLVNAYSIPTDTHEFMTPDGLLSRRAIVELEPFMKAF
                     HQMMSEAEPRGTRWYHPKMGLFQVIGWEGK"
     gene            27587..27925
                     /locus_tag="ACIYSB_000299"
     CDS             27587..27925
                     /locus_tag="ACIYSB_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427424.1"
                     /translation="MPLITQNFRLNALANQYAAALHNHISATNGDHFMINVNGQSIRV
                     NIIGGVKGIRDLIDGYALKAIKENYPQWEAVGIQLLSKCVRGNELTQSGKEIWQSMVN
                     DMSDTVMGNM"
     gene            27926..28150
                     /locus_tag="ACIYSB_000300"
     CDS             27926..28150
                     /locus_tag="ACIYSB_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427425.1"
                     /translation="MKIQYKDYGAVANIVITSSCLTLQKHNRVVDAVLFNVQGIYENR
                     SGFFWVKSSLSGKTRDILRAYKIVQREAKR"
     gene            28722..28880
                     /locus_tag="ACIYSB_000301"
                     /pseudo
     CDS             28722..28880
                     /locus_tag="ACIYSB_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28968..29210
                     /locus_tag="ACIYSB_000302"
     CDS             28968..29210
                     /locus_tag="ACIYSB_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGC5427426.1"
                     /translation="MFALIQRGQIYTDSAGYPVKIVRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            29214..29348
                     /locus_tag="ACIYSB_000303"
     CDS             29214..29348
                     /locus_tag="ACIYSB_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427427.1"
                     /translation="MNKAFELWVRQRYGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            29367..29621
                     /gene="xisR"
                     /locus_tag="ACIYSB_000304"
     CDS             29367..29621
                     /gene="xisR"
                     /locus_tag="ACIYSB_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGC5427428.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            29655..30941
                     /locus_tag="ACIYSB_000305"
     CDS             29655..30941
                     /locus_tag="ACIYSB_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5427429.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            30977..31663
                     /gene="mlrA"
                     /locus_tag="ACIYSB_000306"
     CDS             30977..31663
                     /gene="mlrA"
                     /locus_tag="ACIYSB_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGC5427430.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     TYLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            31723..31830
                     /gene="yohO"
                     /locus_tag="ACIYSB_000307"
     CDS             31723..31830
                     /gene="yohO"
                     /locus_tag="ACIYSB_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGC5427431.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(31811..32542)
                     /gene="yehW"
                     /locus_tag="ACIYSB_000308"
     CDS             complement(31811..32542)
                     /gene="yehW"
                     /locus_tag="ACIYSB_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGC5427432.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(32547..33473)
                     /gene="yehX"
                     /locus_tag="ACIYSB_000309"
     CDS             complement(32547..33473)
                     /gene="yehX"
                     /locus_tag="ACIYSB_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGC5427433.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERADGEALAEEMTLRDALS
                     LFVARGCEVLPVVNMQGQPCGTLHFQDLLVEA"
     gene            complement(33466..34623)
                     /gene="yehY"
                     /locus_tag="ACIYSB_000310"
     CDS             complement(33466..34623)
                     /gene="yehY"
                     /locus_tag="ACIYSB_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGC5427434.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(34630..35547)
                     /gene="osmF"
                     /locus_tag="ACIYSB_000311"
     CDS             complement(34630..35547)
                     /gene="osmF"
                     /locus_tag="ACIYSB_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGC5427435.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLREYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(35758..38055)
                     /gene="bglX"
                     /locus_tag="ACIYSB_000312"
     CDS             complement(35758..38055)
                     /gene="bglX"
                     /locus_tag="ACIYSB_000312"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGC5427436.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            38251..39966
                     /gene="dld"
                     /locus_tag="ACIYSB_000313"
     CDS             38251..39966
                     /gene="dld"
                     /locus_tag="ACIYSB_000313"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGC5427437.1"
                     /translation="MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLRACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(40004..40936)
                     /gene="pbpG"
                     /locus_tag="ACIYSB_000314"
     CDS             complement(40004..40936)
                     /gene="pbpG"
                     /locus_tag="ACIYSB_000314"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGC5427438.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(41110..41697)
                     /gene="yohC"
                     /locus_tag="ACIYSB_000315"
     CDS             complement(41110..41697)
                     /gene="yohC"
                     /locus_tag="ACIYSB_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGC5427439.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            41867..42445
                     /gene="yohD"
                     /locus_tag="ACIYSB_000316"
     CDS             41867..42445
                     /gene="yohD"
                     /locus_tag="ACIYSB_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGC5427440.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(42575..43336)
                     /gene="yohF"
                     /locus_tag="ACIYSB_000317"
     CDS             complement(42575..43336)
                     /gene="yohF"
                     /locus_tag="ACIYSB_000317"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGC5427441.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(43389..44825)
                     /gene="mdtQ"
                     /locus_tag="ACIYSB_000318"
     CDS             complement(43389..44825)
                     /gene="mdtQ"
                     /locus_tag="ACIYSB_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGC5427442.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            45049..45132
                     /gene="yohP"
                     /locus_tag="ACIYSB_000319"
     CDS             45049..45132
                     /gene="yohP"
                     /locus_tag="ACIYSB_000319"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGC5427443.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(45505..46452)
                     /gene="dusC"
                     /locus_tag="ACIYSB_000320"
     CDS             complement(45505..46452)
                     /gene="dusC"
                     /locus_tag="ACIYSB_000320"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGC5427444.1"
                     /translation="MSVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            46691..47089
                     /gene="yohJ"
                     /locus_tag="ACIYSB_000321"
     CDS             46691..47089
                     /gene="yohJ"
                     /locus_tag="ACIYSB_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGC5427445.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            47086..47781
                     /gene="yohK"
                     /locus_tag="ACIYSB_000322"
     CDS             47086..47781
                     /gene="yohK"
                     /locus_tag="ACIYSB_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGC5427446.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            47911..48795
                     /gene="cdd"
                     /locus_tag="ACIYSB_000323"
     CDS             47911..48795
                     /gene="cdd"
                     /locus_tag="ACIYSB_000323"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGC5427447.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            48945..49664
                     /gene="sanA"
                     /locus_tag="ACIYSB_000324"
     CDS             48945..49664
                     /gene="sanA"
                     /locus_tag="ACIYSB_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGC5427448.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            49667..49906
                     /gene="yeiS"
                     /locus_tag="ACIYSB_000325"
     CDS             49667..49906
                     /gene="yeiS"
                     /locus_tag="ACIYSB_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGC5427449.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            50100..51338
                     /gene="preT"
                     /locus_tag="ACIYSB_000326"
     CDS             50100..51338
                     /gene="preT"
                     /locus_tag="ACIYSB_000326"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGC5427450.1"
                     /translation="MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            51332..52567
                     /gene="preA"
                     /locus_tag="ACIYSB_000327"
     CDS             51332..52567
                     /gene="preA"
                     /locus_tag="ACIYSB_000327"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGC5427451.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(52810..53820)
                     /gene="mglC"
                     /locus_tag="ACIYSB_000328"
     CDS             complement(52810..53820)
                     /gene="mglC"
                     /locus_tag="ACIYSB_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGC5427452.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(53836..55356)
                     /gene="mglA"
                     /locus_tag="ACIYSB_000329"
     CDS             complement(53836..55356)
                     /gene="mglA"
                     /locus_tag="ACIYSB_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGC5427453.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(55417..56415)
                     /gene="mglB"
                     /locus_tag="ACIYSB_000330"
     CDS             complement(55417..56415)
                     /gene="mglB"
                     /locus_tag="ACIYSB_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGC5427454.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(56695..57735)
                     /gene="galS"
                     /locus_tag="ACIYSB_000331"
     CDS             complement(56695..57735)
                     /gene="galS"
                     /locus_tag="ACIYSB_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGC5427455.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGAGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(57877..59034)
                     /gene="yeiB"
                     /locus_tag="ACIYSB_000332"
     CDS             complement(57877..59034)
                     /gene="yeiB"
                     /locus_tag="ACIYSB_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGC5427456.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     RDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(59051..59719)
                     /gene="folE"
                     /locus_tag="ACIYSB_000333"
     CDS             complement(59051..59719)
                     /gene="folE"
                     /locus_tag="ACIYSB_000333"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGC5427457.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            59977..60813
                     /gene="yeiG"
                     /locus_tag="ACIYSB_000334"
     CDS             59977..60813
                     /gene="yeiG"
                     /locus_tag="ACIYSB_000334"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGC5427458.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(60845..62834)
                     /gene="cirA"
                     /locus_tag="ACIYSB_000335"
                     /pseudo
     CDS             complement(60845..62834)
                     /gene="cirA"
                     /locus_tag="ACIYSB_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(63128..64597)
                     /gene="lysP"
                     /locus_tag="ACIYSB_000336"
     CDS             complement(63128..64597)
                     /gene="lysP"
                     /locus_tag="ACIYSB_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGC5427459.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(64802..65683)
                     /gene="yieE"
                     /locus_tag="ACIYSB_000337"
     CDS             complement(64802..65683)
                     /gene="yieE"
                     /locus_tag="ACIYSB_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGC5427460.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            65782..66831
                     /gene="yeiH"
                     /locus_tag="ACIYSB_000338"
     CDS             65782..66831
                     /gene="yeiH"
                     /locus_tag="ACIYSB_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGC5427461.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            66905..67762
                     /gene="nfo"
                     /locus_tag="ACIYSB_000339"
     CDS             66905..67762
                     /gene="nfo"
                     /locus_tag="ACIYSB_000339"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGC5427462.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            67765..68853
                     /gene="yeiI"
                     /locus_tag="ACIYSB_000340"
     CDS             67765..68853
                     /gene="yeiI"
                     /locus_tag="ACIYSB_000340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGC5427463.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNTAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(68960..70210)
                     /gene="nupX"
                     /locus_tag="ACIYSB_000341"
     CDS             complement(68960..70210)
                     /gene="nupX"
                     /locus_tag="ACIYSB_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGC5427464.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(70310..71251)
                     /gene="rihB"
                     /locus_tag="ACIYSB_000342"
     CDS             complement(70310..71251)
                     /gene="rihB"
                     /locus_tag="ACIYSB_000342"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGC5427465.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            71381..72079
                     /gene="yeiL"
                     /locus_tag="ACIYSB_000343"
     CDS             71381..72079
                     /gene="yeiL"
                     /locus_tag="ACIYSB_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGC5427466.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(72150..73400)
                     /locus_tag="ACIYSB_000344"
     CDS             complement(72150..73400)
                     /locus_tag="ACIYSB_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGC5427467.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIPQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(73494..74426)
                     /gene="psuG"
                     /locus_tag="ACIYSB_000345"
     CDS             complement(73494..74426)
                     /gene="psuG"
                     /locus_tag="ACIYSB_000345"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGC5427468.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAQGVIGKESTPFLLARVA
                     ELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(74414..75355)
                     /gene="psuK"
                     /locus_tag="ACIYSB_000346"
     CDS             complement(74414..75355)
                     /gene="psuK"
                     /locus_tag="ACIYSB_000346"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGC5427469.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(75778..77469)
                     /gene="fruA"
                     /locus_tag="ACIYSB_000347"
     CDS             complement(75778..77469)
                     /gene="fruA"
                     /locus_tag="ACIYSB_000347"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGC5427470.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     SKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(77486..78424)
                     /gene="fruK"
                     /locus_tag="ACIYSB_000348"
     CDS             complement(77486..78424)
                     /gene="fruK"
                     /locus_tag="ACIYSB_000348"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGC5427471.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(78424..79554)
                     /gene="fruB"
                     /locus_tag="ACIYSB_000349"
     CDS             complement(78424..79554)
                     /gene="fruB"
                     /locus_tag="ACIYSB_000349"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGC5427472.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            79922..81103
                     /gene="setB"
                     /locus_tag="ACIYSB_000350"
     CDS             79922..81103
                     /gene="setB"
                     /locus_tag="ACIYSB_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGC5427473.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(81100..81354)
                     /gene="yeiW"
                     /locus_tag="ACIYSB_000351"
     CDS             complement(81100..81354)
                     /gene="yeiW"
                     /locus_tag="ACIYSB_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGC5427474.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            81509..82081
                     /gene="yeiP"
                     /locus_tag="ACIYSB_000352"
     CDS             81509..82081
                     /gene="yeiP"
                     /locus_tag="ACIYSB_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGC5427475.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            82304..83770
                     /gene="yeiQ"
                     /locus_tag="ACIYSB_000353"
     CDS             82304..83770
                     /gene="yeiQ"
                     /locus_tag="ACIYSB_000353"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGC5427476.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            83888..84874
                     /gene="yeiR"
                     /locus_tag="ACIYSB_000354"
     CDS             83888..84874
                     /gene="yeiR"
                     /locus_tag="ACIYSB_000354"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGC5427477.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRTTPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            84913..85626
                     /gene="lpxT"
                     /locus_tag="ACIYSB_000355"
     CDS             84913..85626
                     /gene="lpxT"
                     /locus_tag="ACIYSB_000355"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGC5427478.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            86038..86604
                     /gene="mepS"
                     /locus_tag="ACIYSB_000356"
     CDS             86038..86604
                     /gene="mepS"
                     /locus_tag="ACIYSB_000356"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGC5427479.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            86785..88341
                     /gene="pdeN"
                     /locus_tag="ACIYSB_000357"
     CDS             86785..88341
                     /gene="pdeN"
                     /locus_tag="ACIYSB_000357"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGC5427480.1"
                     /translation="MFIRAPNFGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            88423..90237
                     /gene="yejA"
                     /locus_tag="ACIYSB_000358"
     CDS             88423..90237
                     /gene="yejA"
                     /locus_tag="ACIYSB_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGC5427481.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIITAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            90238..91331
                     /gene="yejB"
                     /locus_tag="ACIYSB_000359"
                     /pseudo
     CDS             90238..91331
                     /gene="yejB"
                     /locus_tag="ACIYSB_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
     gene            91331..92356
                     /gene="yejE"
                     /locus_tag="ACIYSB_000360"
     CDS             91331..92356
                     /gene="yejE"
                     /locus_tag="ACIYSB_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5427482.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            92358..93947
                     /gene="yejF"
                     /locus_tag="ACIYSB_000361"
     CDS             92358..93947
                     /gene="yejF"
                     /locus_tag="ACIYSB_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGC5427483.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIILRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(93951..94295)
                     /gene="yejG"
                     /locus_tag="ACIYSB_000362"
     CDS             complement(93951..94295)
                     /gene="yejG"
                     /locus_tag="ACIYSB_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGC5427484.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(94628..95818)
                     /gene="bcr"
                     /locus_tag="ACIYSB_000363"
     CDS             complement(94628..95818)
                     /gene="bcr"
                     /locus_tag="ACIYSB_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGC5427485.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(95846..96541)
                     /gene="rsuA"
                     /locus_tag="ACIYSB_000364"
     CDS             complement(95846..96541)
                     /gene="rsuA"
                     /locus_tag="ACIYSB_000364"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGC5427486.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            96690..98450
                     /gene="radD"
                     /locus_tag="ACIYSB_000365"
     CDS             96690..98450
                     /gene="radD"
                     /locus_tag="ACIYSB_000365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGC5427487.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRLAHELRG"
     gene            98575..98859
                     /gene="rplY"
                     /locus_tag="ACIYSB_000366"
     CDS             98575..98859
                     /gene="rplY"
                     /locus_tag="ACIYSB_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGC5427488.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(98998..100005)
                     /gene="yejK"
                     /locus_tag="ACIYSB_000367"
     CDS             complement(98998..100005)
                     /gene="yejK"
                     /locus_tag="ACIYSB_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGC5427489.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELKSLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            100187..100414
                     /gene="yejL"
                     /locus_tag="ACIYSB_000368"
     CDS             100187..100414
                     /gene="yejL"
                     /locus_tag="ACIYSB_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGC5427490.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            100434..102194
                     /gene="yejM"
                     /locus_tag="ACIYSB_000369"
     CDS             100434..102194
                     /gene="yejM"
                     /locus_tag="ACIYSB_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGC5427491.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            102269..102345
                     /locus_tag="ACIYSB_000370"
     tRNA            102269..102345
                     /locus_tag="ACIYSB_000370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:102303..102305,aa:Pro,seq:ggg)
     gene            complement(102448..105039)
                     /gene="yejO"
                     /locus_tag="ACIYSB_000371"
     CDS             complement(102448..105039)
                     /gene="yejO"
                     /locus_tag="ACIYSB_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HGC5427492.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSNNTEINGGYQYIEMNGAAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDTTIKGGRLIVEKDGGAVFVAIEKGGLLEVKEGG
                     FAFAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTVTGVDKKAGGKLIVSTNALEVSGPNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDKHATMQSLGKDTGTKVQANAVYDLGRSYQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGIIEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLIFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            105359..106006
                     /gene="narP"
                     /locus_tag="ACIYSB_000372"
     CDS             105359..106006
                     /gene="narP"
                     /locus_tag="ACIYSB_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGC5427493.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(106217..107269)
                     /gene="ccmH"
                     /locus_tag="ACIYSB_000373"
     CDS             complement(106217..107269)
                     /gene="ccmH"
                     /locus_tag="ACIYSB_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGC5427494.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(107266..107823)
                     /gene="dsbE"
                     /locus_tag="ACIYSB_000374"
     CDS             complement(107266..107823)
                     /gene="dsbE"
                     /locus_tag="ACIYSB_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGC5427495.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(107820..109763)
                     /gene="ccmF"
                     /locus_tag="ACIYSB_000375"
     CDS             complement(107820..109763)
                     /gene="ccmF"
                     /locus_tag="ACIYSB_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGC5427496.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(109760..110239)
                     /gene="ccmE"
                     /locus_tag="ACIYSB_000376"
     CDS             complement(109760..110239)
                     /gene="ccmE"
                     /locus_tag="ACIYSB_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGC5427497.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(110236..110445)
                     /gene="ccmD"
                     /locus_tag="ACIYSB_000377"
     CDS             complement(110236..110445)
                     /gene="ccmD"
                     /locus_tag="ACIYSB_000377"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGC5427498.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVIQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(110442..111179)
                     /gene="ccmC"
                     /locus_tag="ACIYSB_000378"
     CDS             complement(110442..111179)
                     /gene="ccmC"
                     /locus_tag="ACIYSB_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGC5427499.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(111221..111883)
                     /gene="ccmB"
                     /locus_tag="ACIYSB_000379"
     CDS             complement(111221..111883)
                     /gene="ccmB"
                     /locus_tag="ACIYSB_000379"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGC5427500.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(111880..112503)
                     /gene="ccmA"
                     /locus_tag="ACIYSB_000380"
     CDS             complement(111880..112503)
                     /gene="ccmA"
                     /locus_tag="ACIYSB_000380"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGC5427501.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(112516..113118)
                     /gene="napC"
                     /locus_tag="ACIYSB_000381"
     CDS             complement(112516..113118)
                     /gene="napC"
                     /locus_tag="ACIYSB_000381"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGC5427502.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(113128..113577)
                     /gene="napB"
                     /locus_tag="ACIYSB_000382"
     CDS             complement(113128..113577)
                     /gene="napB"
                     /locus_tag="ACIYSB_000382"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGC5427503.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(113574..114437)
                     /gene="napH"
                     /locus_tag="ACIYSB_000383"
     CDS             complement(113574..114437)
                     /gene="napH"
                     /locus_tag="ACIYSB_000383"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGC5427504.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(114424..115119)
                     /gene="napG"
                     /locus_tag="ACIYSB_000384"
     CDS             complement(114424..115119)
                     /gene="napG"
                     /locus_tag="ACIYSB_000384"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGC5427505.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(115126..117612)
                     /gene="napA"
                     /locus_tag="ACIYSB_000385"
     CDS             complement(115126..117612)
                     /gene="napA"
                     /locus_tag="ACIYSB_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGC5427506.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(117609..117872)
                     /gene="napD"
                     /locus_tag="ACIYSB_000386"
     CDS             complement(117609..117872)
                     /gene="napD"
                     /locus_tag="ACIYSB_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGC5427507.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(117862..118356)
                     /gene="napF"
                     /locus_tag="ACIYSB_000387"
     CDS             complement(117862..118356)
                     /gene="napF"
                     /locus_tag="ACIYSB_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGC5427508.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT        29183 a        31832 c        28707 g        28722 t
ORIGIN      
        1 agttcaccac ctgttcaaac tctgcgctga actcaacacg cagcatactg acccgcgacg
       61 accattttgc gcaggtcacc tttatctgcc gccactcata aggcggcgtc cacagaaagg
      121 ccttccagcc cccgtgctca gccagaaacg actccagcgc cgtggcctcc caacggggaa
      181 cagaaagcgt cacgctgtac gttttcaggt cagcgttcag cccggcaggc gctcgctggg
      241 aatagccatc accaaagcgc actttcctga cggaaggggc cgaagccaca tccataccgg
      301 gtttcacttt ccagcggaag gttttcatcg tccacctccg gagaacagac caccatcacg
      361 ggactgctgt tgcataaagt ccgctgctgc ttttttcccg aggtcataaa ccaccttcag
      421 ggcagccgga cctatctgcc cgttcatgcc gtcgttatta atctcgatat tgtactgcgg
      481 ggcaaacatc gccatacctg atccacaaat ctgtgccaca acacccagct taccgtcagc
      541 accacgacgc agtggcagaa tggcttcagg tcccgcttct cccatcacac ccgccccttt
      601 tgcaaaagca aaaaacgtcg gacggttaac caccgtgcca ctgtaacgac tcaaatcagc
      661 agactgataa atccccccat tggcattcag ggaaaggctg ctgagatcaa atcccagtac
      721 actgccaatc ccttttatcg atttcatcat ggttgcctgc gccaggattt tcgccatatc
      781 tgacagcacc gaagaggtga aggatttgaa gttgagtttg ccggtagtga caaaagttgc
      841 caggccattt cccatgttgc tgaaggctga tgagaacacc tgttctgctg ttcctgccgt
      901 attgtctgca tccacggtaa aatcctgaaa tgcacgcagg actccgtttt tccagttacc
      961 ctgcacaact tcaagctgtt gccagtaacg gcgattctca ttcagttgtc ggttcaggct
     1021 ctccgtcagc gcctgctcgg cctttctgta gtcatccgtg ttatatgtcc ctttctgctc
     1081 actatcccgc ctcaactgct ccagctgttg ctggtatttc tggcgaagac tcagttgtac
     1141 ctgatatcgc tgccgctgct gatcacccat acccaccgtg gcgatatcca ggtcatgttg
     1201 ctgacgctga gcgcgctctt cttcagccag ttgactggtc agctgaattg tttttttctt
     1261 cagatcgttg agtgccgttt gtttctgaag ctcctgctgt tttacatcca gcagcgtcag
     1321 tgcctgcatc agttcatctt tacgggccag cacactcttt tcatctgccg tcagtttttt
     1381 cccgtccaga tcgctgatgc gctgctgcag agccagaagc tgtttatgcg cttctgtcat
     1441 cttttcagtg gcaatgcctg ctgactgcct tgcagcagca atctgtcctt ccacctgtgc
     1501 ctgttgctga ctgtactgca gcaataaccg ggtggcctca tcattacggg tttcgcgtgt
     1561 ttttttctta ccggatgcca gggctttctc gtaacgttca ttttcacgtt gtatcgccgc
     1621 atccctgaca gcctggtcgg cgtactgcat ggcattaata cgcgcaattt cacgctgatg
     1681 tcgtgctgct tccgtttcat tcatccggtt cagtgcagca ttttcagcat tacgtcgttt
     1741 ctgttgctcc tgataattcc gctctgcctg ctcttttgca tcctgcaaat ccttctggcg
     1801 ttttttctcc tgaagatcgt taagacgctg ctgatcgtat tcaacctgag aagatgatgc
     1861 cgtccagggg agtcttttcg cccgcgacac tttctcctgt aaagcggtaa tctgttcatc
     1921 cagcgagtct tcacgaccaa tattcatggc cgcatcccag aaacgactcc acaaatcaga
     1981 cagatacttc agcgtactgc caagcgcatt gaggttatta tcaatatccg cagtacgccg
     2041 accggtttcc tctgccagtg cagacatggc tatccgtgca gcatcactgg accgtccctg
     2101 atccccaagg acacgtatct gctcaagctg agtggcagta agaaaatgca gctcattgtc
     2161 cagagccttc gcggcattta caggatcatc cttcagccgc ttaaactgat ttatggtatc
     2221 gctgaccgac tggccaaccg atcgctccat ctgtgcggca gctctcgcca ccataccgat
     2281 atcgtttcca cgaaatgcac cactccccac cacctgcgcc agcgcaccgg ctgcagcatg
     2341 ttgcgtgata ccattcccgg aaatagcacg actgagcgtc cacagctgcc cggcagtgac
     2401 tccggcataa tgccccgtca gcgacaactg gcggttaaat tcttccccct ccttctgacc
     2461 gtcataccag gctttaccca gaccatagac ggccgcggca ataccgccaa taaccccgcc
     2521 aagcatcatg cctttcggtg acatcagtgt gtctatccat ccggcacggt tagccagcgt
     2581 tatcccggat cccctcagcg cccctaaatt gccgcgggcc agttcaccta tcagaacgcc
     2641 tatctcctgg cgggctgctg cacttttcag acccagcgaa tgcgtggctt ttcctgcctg
     2701 ctccattttg cggatataca cttctgcagc actgcttacc cccagctggg ctgccttggc
     2761 acgaagcaac tcagaagatg aaagattctg gcgggttgcc tgctctttaa gctgacggat
     2821 aaacgccact ttctgtcggg tagcctcttc ctcagcctgt gtaagaacac gggttttcgc
     2881 agtaacctca gaaatcagcg ccagataatc ctgctgacca atcccgccac tgtttctggc
     2941 ctgtcggatc tgctgctgaa tacgctgtaa ttcctgcagc cccgcactgg cctgtttcac
     3001 actgtcaatc tgacgataaa acgcagcagc cgctttatcc tgagcctccg ccagagccat
     3061 ggcctgcgcc tgttcctcgc gcattttctg gctcagcgcc tccatgcgct ggcgggtttt
     3121 ctccacctcg cgggccatgc gttcatgagc ctgtgcgttc ttctccaccg tctgcgcatg
     3181 gacggatgcg gctgttgcag ccgaagaagc cgcctgcgtt gtctgccggg cggcctgagt
     3241 ctgacgctcc ataaaacgct gcatacgggc agaagaccgt tctgcatcgc tggctgcacc
     3301 attcagaagg tttttgatac ggggaatttc atttttaaat tctgccgcat caatccccaa
     3361 atcaatgacc aggttggcta tctggtccat aacgcacacc tccggaaata ccttccccaa
     3421 gatgcatcag ttcttcgtcc gttcgctccg gtatcccgtt ctcttccggt aaaaggctga
     3481 aatcagccac cgcagcatca ctgctgccgg acaccattct cacgatcaat gccttcagcg
     3541 aggcaaactg cgcatccatc cacacatcac tgaagctctg catccggaaa taatcgcccc
     3601 actcaccaag ctcagtggcc gacatttccg acagcatccg ccgccagtct gcccgcctga
     3661 actcccgggc aagccgcatg acaaactgca tttcccgcgt caggactttt ccggcgtcag
     3721 cacctcatgc tccaaatccc cggcattctc aatggctccc ataccgctca gcgaaagaac
     3781 catctctgcc cccgcaccca gggcatcata cgaccatgtt gtaataacgg atgcgtaaag
     3841 cgtctcaaca tcctgagact gttccgcatt ccacagtgag cgggaaacca gccaggcatt
     3901 gatatccatc cccatccgca gaaaagcaat ctgtcgttca gcctccggca gttctccctc
     3961 cccggcatca aactttgccg ttcgctgctg aacaaacgtc agatattcaa ttctctgcag
     4021 cccggacagc tcactgagca ccacggactg tttttcataa ttaaacgtgt cctgtttcag
     4081 aaacatcatg ttctccacct gcaaaaaagc cccgaataac cggggcaaat gatgagtatc
     4141 gtcctgttaa cctgctgcgc tgacagtcac cgcagccaca gccacaaaat tcccgtcagc
     4201 ggtcatgccc acaatgctga cactgccctg cttcacgcct ttcaccgtgg ccacaagccc
     4261 gttcagggtc accgtggcag tctgtggatc tgtcgaatgc acactgatcg ctttgtcact
     4321 ggctccgtca ggttttactg taaaggtcag cgtggtggtt gctcccactt ttacactggc
     4381 agataccggt gatattgtca gcccggtaac gcccacggtt tcagtgcctt cctctgccag
     4441 atacggacgc cccacaccgc tgattttcac ggtgcgggtc atcacgtctt ttgaggcaat
     4501 ggttttaccc agtgagctca gccagccacg gaaaacatca acagtgccgt tgggatattt
     4561 gatacgaaac gcgcagactt caccggagtc gaacaactga accagttttt tctgcccgct
     4621 gtcacccgga cgccaggcca gcgtcgccga agtatcacca acggatttct gcccctgggt
     4681 tgttgttttc cagtctgcat cttcatcatc gagataagtg tcatcttctg catcagcggt
     4741 catttcgcca ggttgcagat ccttcaccat cgcaagacgc agccagtcag tgtcctgtaa
     4801 agggttcgca aacggatcgc cacggccggt gtacatccag aacgtcgttc ccgcaccttt
     4861 cgtttttgcc agtggatttg gtgtggtcat tgccacctcc ttaattcgtg tacgtgatct
     4921 ggtacgtgat ttccgccatc gcccaggtgg ccatctcatt atcacgttga tagttaaaac
     4981 cgagtgggat cagggtatcg atgagtccgg aaagtgccgg tacatcattc agggccggga
     5041 aaatggtgct ctccatccac atatccagct ctgaatccgg tgcctgtgcc cggatgaaga
     5101 cagcaatatg cagaacagcc tgccagtcat cttcatccgt catttttccg gtgtactgag
     5161 catcactcag ccacaccgcc acggcaggca gttcctgcgc atcaacaaat gccggaagcc
     5221 cgtcaaaaaa agtggcgctg tctccacact gttcccgaag gcgtgccagt acgacctggc
     5281 ggatttgtgt atgtcggttc atcgggtcag ccataacctc agttgttgtt tcagtgcata
     5341 ccccagctgt ttcggcattt ccgcagcaat gatgcggtcg cgggcatctt caaatgcctg
     5401 tgtcagcggt ccggacagcg ggattttcac tacctccaca ggaagacgat tttttcttgg
     5461 cctgccctga tggtcatgcc ctgttgcaaa acgcgcttca ggaagacgcc tcagaacatg
     5521 ccagcggcca ttcgccagtt gctgaacaaa tgccccccgg aaaaagtatt ttcccactct
     5581 cagtccttca ccagaacgtc gccgtgttgt gttcagtttg atggcgggca gattgccccg
     5641 gttaacgcgg attctggccg tcatttttcc tgacggactg gctttaaaca cccggacacg
     5701 ctgacgtacc agtttcaggg ggatcccttt cacctggtta tctcccgcaa cggtattccc
     5761 ggcaacctgc cgggtggcaa ccgagaccgc tttctgtgcc acacggttta tcgcccatgc
     5821 gctggcctgt gacaccatac gggtatcaag gctgttcaga ttgcggatag cattctcaag
     5881 ccccttcatc ccacacatct ttactcaata aagatcattg tcttaccgtt aaaacgttca
     5941 tgccgtgtga ccgtccattg ttgtccgtca taaacaacgc gatccccgcg ccgtgggcgg
     6001 tatcccgaag aaaacaccac cagagagacc gcaggtccgg acagagcatt cagctctgcc
     6061 agtgtttctc ccgggatcac agtcatatcg acatcattaa tacaggccgt tcttcccatt
     6121 ctcctgacag ttaccgagtc catacgggaa cacacagact gaaagggggg catcatgaac
     6181 caccccgatc gcctttttcg cccttcggcc cctgtggtcc tgccgggccg cgttcacctg
     6241 tttcaccctt tggcccctga ggtcccggct ctcccttttc tcctctcggg cccgtgtccc
     6301 ccttttctcc cctggcacca ccaaagttaa tcctgacagc gacgacatca ctcccggtac
     6361 cagcctcttc ccagacaacc ccgaccagcg gatcagagcc gctgttagtc agctgaactt
     6421 ttccggactt cagataaacc ttcttacccg ttttcatatc atccgttttc agcttaggca
     6481 gcataaacac accttcggtc atgccgtcgc ctgtttcacc ctgtggaata tcggtcagcg
     6541 ccaccgcaaa aacatcaccc acctgcacca gatctccgct gctgatggct gcactggcaa
     6601 caatcgccac cgtttttcct tcttctacaa aattctttgc cataactgtc tccacacagc
     6661 cccgttcagg ggctgatttc aggtacaaaa aaagccctta cgggccatca gagttgttgt
     6721 ctgcgatgtt tacgccgtac atttcaccag accgcggtga tcaactggcg cgacaccggc
     6781 gtcaatacgc actttcgttg tcacgccatc cacactgaag ccctccatct gatcaatata
     6841 tggcgtatcc acaccgttga gataagccac ttcaatcgta tcggagcctt ttgacgcagc
     6901 caggtagaag gtggtctggc tgttatcatc aagacgaggc tctgcaataa cggtcgcaaa
     6961 atctttcacc gggttaataa taccggcgtt aatgtcagcc cccttgacac ttgaggagcg
     7021 aatgacctgg ttagcaacag actccatcgc cgtcggtacc agtacgaacg caggacgaat
     7081 attcagatga cgctccccct ctttctgaac gcgcatcaac tggcgggctt tatccagcga
     7141 tgccacgtcc attgcagcgc tctccagtac gtttgcatgt ttcgctttat cgaacagact
     7201 tacattatct gtggagattt tcgggttaga cgtcagaatg gcataaacca gatcggcaat
     7261 agtggatttc gccgcacggc ccagtttcat cgggacatcg gtcagcatat tcagatcatc
     7321 attgataatg gcctgacggg tgatactgaa cagctcgcca taggtcgcca gtgcaatagt
     7381 ggcctgttta tctccggtgg tgacgtattt atattccgcc ccttcacgca cctgacgcag
     7441 agcactgaag ccccccatac ccacacgatg ggcaatttta aaatcagaca actgaccttt
     7501 ccgcgtccac tgttcatagg tttcaggggc atcttcccag ccctgcagaa tggctttgtt
     7561 cgcaacatcc agcagaatat taccgaagtc agacgtactg tgtgtgaacg ccgcaccgac
     7621 catctgcatc gggttataac cggaaacccc aataccccgt tcagttagtg acatacgggc
     7681 atattcacgc agggtcatcc cgttgtagac attatcacgt tcggtttttt caaatccggc
     7741 acgcgccatc agcgcctggc gaatcccgtc ccccacaaaa ttaccgttac cggcataaat
     7801 atgagccggg gtatttttat tggatggcgt ggactcgcgc cccatctcgt tcaacagctt
     7861 ttcacgggcc tgctccagcg aacattcagg atcggcaaga cactgagcct gcagcgtctg
     7921 ataacgcccg ccaaacatgg caaacagatc attaataccg tttacacgcg ctttttgctc
     7981 tgccagtacc tgcgcacgga tgctgttttc atccaccacg ggtgctgctg cctgcactgg
     8041 cgtccaggag gcttcagatt cattatcctg tacgcgtgga gcactgttgc gtggcggagt
     8101 aatcatgttt cgaatggatt ccggcatctt tttaaattcc tctgtacgtt ttgactgaat
     8161 acatgccatt gccttaacgg ctggcgttac ctgatcagca aatccatgtg ccagacattc
     8221 ggcaccggac atccaggtct catccgccag catggcagca atttcatcgg tggttttccc
     8281 ggttttctgt gcataagcgg gtaacagaac cgcctcaact ttatcgagca ggtcggcata
     8341 ggtgcgcatg tcctccgcat caccgcccgt aaagccaaat ggtttatgaa tcatcatgaa
     8401 ggtgttttcc ggcataatga ccgggtttcc taccatcgca atgaccgacg ccattgacgc
     8461 cgccacaccg tcgacataaa cggtaatgga cgcaccatgt gttttcagcg cattaaaaat
     8521 ggcgatgcct tcaaagacat cgccacccgg tgaattaata tggagattaa tgtgggtgat
     8581 atcgcccagt gcattcagat cactgataaa ctgcttcgct gtaacacccc agaaaccaat
     8641 ctcgtcataa atataaatat ccgcgtcact ctggtgacca gcctgcatcc tgaaccagga
     8701 attattcttc ggactggtcg tcggtgtgct gcggctcctg tcgtttcgtt gcggcactac
     8761 tgcctccttt atcactggcc ggatcggtat caaataccag atccagcttg cggttttcat
     8821 caatttcggc cttgcgccga cgtttgacat catccggatt acgaccacca gcacgtaccc
     8881 agtctgattc tgtcgccgct ccaccacgaa tctggatttt ccaggcctca gcctccttaa
     8941 cagggtcaat ccacggcatc actggtccgg aatacaccgc ggtatacagt gaagaacggt
     9001 caagagcgcg gggtagcctg ataacaccgg atgccacagc ctgtttcagc catgcacgat
     9061 acatcgggcg ggtgacggca ccaataaacc agtcctgcag gatcaggtag ccatcagtgg
     9121 attcaaccag ctcctgacgc tgggcgctgt aagtgccgtt atagttgcgc gctgtactgg
     9181 aaaaactcag acgactgccc gccgccacgg cacgcaactg accattacga aaagtttcaa
     9241 ggttaggatt ggggcgatcc gacttcacca ttccgatttc ttcgccgggt ttcagatcgt
     9301 cgtaaataat gcccggctga atggtaagct cacgttcatt ctcctttctg ccattaccat
     9361 ccggttcata gctctgcccg tcgcctttcc ggatgtacat ccccagagca gcggcgatcc
     9421 ttgctgcagt cacctcagaa tcttcatatt ctttcagggc actgaggcgg atcagcacac
     9481 cggacaacaa agacgtcccg cgcatctggt gcagacggcg aacaaattta agatgcagca
     9541 ttcgctctgc atccacttct ttggtttcca tctgccgtcc ggatacggga cggcttttat
     9601 acaccagata tttttcggga cgcccccagt catcaacaaa cacgccctga ttcagcctgt
     9661 tgctctcatc actggtcatg ggaataaagt ccggctcgag cgcctccagc cagaaatgaa
     9721 caccggcaga aggcgccagg ctgtttatgc gcccggaaac catctgggca aacacctcac
     9781 catcgcgcag ccaggtacga agcatcagac gttccagcat cggacgggta aactgcccgg
     9841 tgacttccgg gctgacagac cattcactcc atcgggtgcg aatctccgca gccaggtcac
     9901 gggcaatggc cccattgcgt aataccggat gtggctcgac aataatcccg tttttcccca
     9961 ccacccgttc ttccagcttg tcaaatacac caataaccag atcgtggttg ttatcaaggt
    10021 aacgggcctg ctcacgtaac gacacggccc cgtactggct taactggtcg gcagttcggt
    10081 tctcccgtcg ggctttgtgt gtccgcgtcg tttttacggc ctcataagcc tggatcaccg
    10141 cacgggaacg cagccttgcc gctttccatc ctggtgaaaa aacgccaatc acatcatcaa
    10201 gaattgccat cagaacctcg ccagccggta cccgggatgc ccccgtcgtc gtgtaatcag
    10261 agccgcaagg cggcgctccc actcctgccg tccctgccgg atctcagata agttttccat
    10321 ggtcatctgc tgaccattaa aggtgacgga ttttccgtcc agcaccgcca tttcagcttc
    10381 cgtataacgc tgaatcatgg cttcgatatc attctggttc ataaccatcc tccggaagtc
    10441 agccaggggt taacatcgtc agttactgtt ttcttccgtt tttgtttttt aacaggcgtg
    10501 gataccggtt ccggtgagga tgacggttcg gtactgtcct ggacacactc cagccaggtc
    10561 tcccggctcg cccactccgg tgcatccggc cagcggatct tttcgtatcc atgcagaatg
    10621 accagagcct cggcatacac catcaggtca aaagcttcgt tggcaccgcg acccggctta
    10681 ctccatttcc cgtcactgct ccgctcttca tacgtcagtt cgtcgtaaaa ccagctcccc
    10741 agccagtcag ggaaatgcac atagcctgga cctggcgagt cacgccataa cgcgttattc
    10801 acccggtctt tcagggcatc cgtctgaaga agccagagcg gcacatcacc tgcggcctgc
    10861 gcccggcggc ccgttcgtcc ggtgttatca gggaatgtac gggtgatcag ttttgctcgc
    10921 cggatgctgt cgcccttaaa caggtaaata cgtttaccaa gtccatcacg acggcaacga
    10981 cgccagaatt tataggcatt atcagtgacc ccgtcttcac cgccggagtc caccgccatt
    11041 gccatcagtc gcatttgttg agaaggatcg gaggccagcg gccagctttt atgaaaaaca
    11101 tccgtcagca ggacatccca gtcttccgga tagctggccg gatcaattcg ctggctctcc
    11161 ccgtcgctgt caccgcgcaa tgactgcgtg atgttgtaac gatcaataat ccagcgttcg
    11221 ccacgactgc catagcccgt tacctgaacc acaaaacggc gatgacgtcc cgcctgcaca
    11281 tccactgtcg ccacaaggaa attaacgcca tgcggcacac tgcgtgaagg aactggctct
    11341 gcccgctgct caagcagttc actttttcgt tgctccatgc tggcgcgggg aagataaggc
    11401 aatccccagt cggtgttgat aaccgccctg agtgtttctt cgcttcctgt cgcttcatac
    11461 tcctgttctg cagtcagtaa tttgtaaacc agttgcgccc aggtctgata cgcagcagct
    11521 ggcccttcca tccagaagct ggcaatcctt gaattacgtg gcgttccggt aacattcccc
    11581 tccatgtcaa tctgctcacc atcccgtaac cagaccccac gtccgttcag ttcacgtttc
    11641 atgtctgagg tgattcggtt accacaatga ggacataata tatgagccga tttactggct
    11701 tttacaggat caggattgtc acgatacccc gtcatagtct ccatggatgg ctgaaaatac
    11761 cccccacaat ccggacaggg ccagtaccag cgacgacgat caccacggtt atagagtgac
    11821 aggatccccg tagtgggggg agcttcatgt ggagaggttt tccgccactt actgtccgaa
    11881 atttcgcgtc cgggtgaact ctccacaagt gtcatccctg ctgacataaa cgtcgtggta
    11941 cgtttggaag ccagggagaa accatcacct tcaccatcaa tatcatcagg ccagcgatca
    12001 taatctgtta atgctacaaa tcgataatca gaagacgaca ttatattaac tgacggccaa
    12061 ccaattttta gataattacc cgccttaaaa gtcttatcat ggacgttatt atcattggtt
    12121 cgtgggctca ttcgccgaac aacctcttta ctgacccgga atgtgcgatc aaggcgtttt
    12181 ctggaatgct cccttgcttt ctcttctgtc atttgaatta acaaaaagtc tgcaggttca
    12241 caaaccacgc tgtaaacaat ccagccatca attaatccaa ccgtttttcc tgttcgcgct
    12301 ggcccgacaa aaacaaccgc atcatagtca cgggaagaca aacaattcat tggctcaatg
    12361 atgtaaggcg tcaaagatgc atcccatggc aatgaacttc ccccacccag tggcactcgc
    12421 atatatttct caacagcttc tgccactggc attcttgtgg gtggttttag taattcggaa
    12481 acatcacgcc tgatagcact tgcagaagca aaggtcataa ttccacctca tcatctcttt
    12541 ctctcatttc agttgataat gcctcacgga tatcatcaac tgctcgttga gcctcagcga
    12601 tttgtgatgc actccagcct cgatctcgtt ccagtttgtc aggccacgta tccagtacct
    12661 gagtaacgac cttcaacatc aacgccattt cgcgatgtac gtcactggca ggaacaagtt
    12721 gttttaatgt cgtttccatt ttgatacgtt cattttctga ctggaaccag tctttacgct
    12781 ctttaggctc cattcgtgac gggtcatgta tatcggaagc aatgactgat ttactctcaa
    12841 acaaagcggg tccgacatct ctcaatgcat aaacaggatt accacgaaca gtaccagcaa
    12901 cagggatatt ggcctccagc agtctttttt ttaccacctt cctgtccata gcaaaggctt
    12961 ctgcaatttt cgcgatactc cagtgataaa cgtctccaat attactgata ttcgacatcc
    13021 ccacctctcc acaccagctc gaatatcatt tttctcatta aaaacaggac attaaaaact
    13081 ggtcagatgt cccataaaaa gccaaatggg acaggtcaaa agcttttttt gcagtaattt
    13141 tcaataggtt acttgatgtg gtggtgtccc atgaaaattc aaaaatcagc cgttttccgc
    13201 gacgctcccg ccccgtggca ggccacccca ccgggaggac ccgtcagcct gacagccatg
    13261 acgaacgtct gatacagcgc cttgcatgaa tggcatcggg ataatccaga aaggaatagc
    13321 atcgtgccca caagaatctg tgtgagtatc ctgtttcttc cacccccgca caggactggc
    13381 gagcatgagg gacaaacccg cgaaccataa acgcggtaaa aacccggtgt gcatcgtttt
    13441 tgattattcc cgcacactct cgcagaagga gttccccgtc gggctacggt catggttaat
    13501 gcaggaatac ggcgacgata cagcgcataa tgtgtcaggc tcgaatacct ttatccgtta
    13561 aaagggatat cagttaagtt atcccgtgta gggtataagc cattatcaaa gccactctgt
    13621 agggagtggc ttttgtaatg gcaataaaaa gccccgcgaa tgcgaggcta aatccaggta
    13681 tttttaatga ctggctctta tctcaacgca gccccttacc gcgcgccaga tgctcaactt
    13741 caagcatcag caatgagatg tttaatctgg attcactcca gaagtgatca ccaccctgtc
    13801 tacagagcca gatgtgaagg atgatcagta aaattatcgc tatcatcgaa ggcattgcgt
    13861 cctgatatat tcctgaagcg ttctcagtgc tgtttggtcg cggataattc cgtcccggat
    13921 accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat tgcccatgcc
    13981 ggaggcgctg gaggtttcgg ctgaggatgg cacagagcat tttcctttga cgagcacccg
    14041 accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat cagccaactc
    14101 cttcgtgtat ttagcatcga gtacatcagc agcacgctgg cgttgctgca tgtcagtaat
    14161 ggtggcggtc gcctgcttca gctcactgac ttttttatca cgctgttctt tgtaggcgat
    14221 ggcgttatca cggtaatgat tgaccgccca cgacaggcag acgatgatgc agataaccag
    14281 agcataaata atcgcggcga ctctgctcac tgatctatcc cccaacaggc taatgcgctt
    14341 tcctggtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg gcaatcgcgc
    14401 ccaccatctt ttatccacca gcgaatcgcc tcgcatgcac ctttacgatc accagcattc
    14461 agccgcttat aaaacgtcga cggaaaacac ttaccggggc caatgttata gggacaaaat
    14521 gacgcgatac ccgctttttg tggctcggtc agtggtactt taatattgcg ctccacccat
    14581 gccagcgcct tatcacgctc aatggcgttg acctggtcgc atttttcctt cgacagtttc
    14641 ataccgggaa aaacgggttt tccatccacc atcgtggcac cccgacagat ggtccagatg
    14701 ccggacccat cgcggtatgc cgttgtgtgg ttaccctctt tttcgtccag aaactgatca
    14761 agtatctgag gagcagatgc gccagcacca atcagcgcca gaacggcagc cgacaggccg
    14821 tatctgattt ttgcgttcat gtatatttat caggatctaa aggtttataa ccctggatat
    14881 atcaggtata taatccagta ccagcatgtc tcataaacat acccgttcag gaaaaccaaa
    14941 cgacaacaga tgaggcttca gaaatgaaca gtaaagaaaa tttgcgcagt agatttttgc
    15001 aactaatgac agaaaaagtt aaatcagcgc tacttccact tatggtggat aataacgaag
    15061 caacgagcag catccttgca gacccttacg gtaaactctc gcataaaacg ctggatatta
    15121 ttatgagtgc attaacaccc cgtatgcttc aacacctgaa acaagatatt aacgcatggg
    15181 tgaatgaaga attgagcccc ccctgttcat gggacagaca ttatgcatgg cagcaaaaaa
    15241 tgaggatttt caattcatta tcactaaagt tcagagaacc gaaacagcgt cccttccctc
    15301 tggaatcaga caaaaaacaa cctgatccca caggccagta atattttgaa taccgtgatt
    15361 attgatatca caatgaaagc tattccgcac agttatgccc tgctccccac ggggcatgcc
    15421 tcatgaaacg ttccgggcgt ttcatgttcc cggattttgt taaaaggtat attatcccct
    15481 cactcccggg atattcgtca tatttctttt ggtgcctttt tcgcaatctc agccacagca
    15541 tgtgccgtag ccggaacatt accggcatta gttcgttcca gaatttcgag caatatgcgg
    15601 gttcgttttt cctgctcccg tttgttaagg cgataagtaa gaacgccaag aatgatactg
    15661 aacgccacgc cgataataaa tccccaatcc tgcagtgaca ggctggcaaa gaacgcaaca
    15721 agtcctgcgc tgccgtatgc agcgttactg tatcgttcgt ccattctcat agtccctcac
    15781 ctccgccatc cgacattacg gacggcagca agtcagaatc tcgttataaa aaagccgcac
    15841 gaagaggctg tttgttcagg tattttatcc agtgcgccgt aaaaccccga ccttcagggt
    15901 gtggaggatg tcaacatcaa taactgacat tctgcccaaa gctcccgaag attccctttg
    15961 cacaatttca ctgaccggat ctcagcacaa gccacaggga atggtcagtg tgacgctctc
    16021 tgtggcccct cctgaaaagc cctgtggttt aatcgcgacg gacctggcgc ggatatgaag
    16081 caggccgtga gcacgagcct ggaaatttag tgccgactac cggaattgaa gtggtggcct
    16141 gatggttaca actaaactgt tctgccgact gaggtaagtc ggcgctggac cgccagcggg
    16201 gaatcgaacc tagcgcactc aacttaaagg gttgacgctc tttcctgatg agctagtagc
    16261 ggttggtggc ccttgctgaa tttgaaccag cgcccttgcg attatgagtc gctcgctctc
    16321 accactgagc taaagggcct cgagcagcat aataacgtta cgggattaat accgcaaatc
    16381 tcagagaaag cccgacaaac ccaactaagc gcacctctta tctggtccgc caaagagctt
    16441 aatgtccggt gcagattttc gatgtaaaaa accgcctggc tgcgggttta agttgtgtgg
    16501 cgtaggaacc actcttacca tgatatgcat acttttacga tcgtaaagtg tttgttcata
    16561 ccctgcatgc tgcatgattc accccacaga caatctctgg attgttgcga cgtttggcaa
    16621 gatacaatta aagattatgc attcttctat gcacgtattt ttcttcaaaa gtaatgtctt
    16681 tattaaggtt atataaataa ctcataaacc caaataatta aatatattta gcacttctaa
    16741 taaaaaagat tgatcttaat tttctgagtg aaagatagcc atctatttat tagtttgcat
    16801 gacttcttat tttgtgatag tgatcacata taaagaatcg gactccttaa ttttccttgt
    16861 atacattata cacagaaatt aaattttcat cctgatagca ttttgtcgtc caattaactc
    16921 ccaccagagg ataaaaaatg aaaaatttaa cagtggcact ttctgctgta gcagcatcag
    16981 tatttctggc aacgtcttca aatgcagcag aaatatacaa taaagatgca aacaaagtgg
    17041 atttatacgg taaagcagtt ggtttacatt atttttcttc tgataagcac caaaacggag
    17101 atcagactta cgcacgcctt ggattcagag gtgaaaccca aataaataat tatattactg
    17161 gttttggtca gtgggaatat aatttccagg ggaacaaccc tgaaggggat ggagctcaaa
    17221 ctggaaacaa gactcgcctg gcatacgctg gtctgaagct tgcagatgct ggctctcttg
    17281 attatggtcg taactacggc gtggtgtacg acgcattaag ctacactgac gtcttcccgg
    17341 agtttggacc taccgcagca caaaaggata acttcttcgt tggtcgtgta ggcggtgttg
    17401 ccacataccg caacactgac ttttttggac tggtcgatgg gcttagcttt gcaattcagt
    17461 atctgggaaa aaatgaacgc aaggataaac cagatacttc aaacggagag ggtgtaggtg
    17521 gctctgtcag ctatgaatat gaaggatttg gttttgtcgc agcatacggc gctgctgatc
    17581 gtactaaaaa tcagaaaaag gctgagcttg gtaaagggaa aaaagctgaa cagtgggcca
    17641 ccggggtaaa atatgacgca aataatctgt acctggctgc cagttatggt gaaacaaata
    17701 atacccgtgc aatatcgtat ggtgataata tttcaggagt ggcgaataaa accgaagatt
    17761 tcactgttgt ggcacaatac cagttcgact ttggattacg cccgtctctt gcttataccc
    17821 actctaaagc aaaaaacctt tcatccagta ctgatattag taaaaaacat gatgacggat
    17881 atattgaaaa attcgttgaa gtaggggctt cttattactt caacaaaaat atgtctacat
    17941 acatagatta caaaatcaat atgctcgata aaaataaatt cactgaaaga acaggagtat
    18001 caaccgacga tattgtcgct gttggtctgg tttatcagtt ctgatttatt ctgtaagaat
    18061 atgccggggg agtgagaact tttctggcat ataaagtggt gccctcatgc cactgcaaaa
    18121 cgttggacag tacttaactt ttccaacgaa ctttctgtta tttattaacc tttttggttt
    18181 ttatacttgg gcgctttgcg cccatttttt attttgtacg ccttatatcc ctgccctgga
    18241 ggacgcagtt ttacgacgta tcgaataatt tgtgctcaca tatcgatcca tctcaaggcg
    18301 aacatctagc atcatcaaca taccatcaac cactccttca gctttttgca aacgctttcc
    18361 aatatgcgta tccgaacatc catgtttcat tgctaacttc atgaacgtca tcccatatac
    18421 gtagtaatca accagcatgt catgcatatc ggcgttgttc tttttcaggc gagccataca
    18481 accacatata atcatcgcgt catcatcgga acactgcgat cgcgatttta cttttgacgg
    18541 tatcagcccc ttaaatccgg cggcaatgcc tgaccattcc acgtcttcat gattatttgc
    18601 gacccatgcc ccccaacgtt caagaaccat ctgaatatca cgcattattc acactcccca
    18661 accagattga ggatgaccgc cgcaccattg tctttcatat attcgccctt tccgcttgcc
    18721 aaaaaccaac gacacacctc cacggcttca gcccgtgtca ccggtttgat ggtcatcagc
    18781 aatttttcaa ggtagcgctc gcggtcatat accgattcgt gatgctcaga gtaaccatac
    18841 tcatcgccct gttctttagt cgcagtgtgg cgaacactgt agagccagtc ccagtaaaca
    18901 aactcacgaa ctacgtctga caatgtatga ggctctggca gtacatcacg atagccatca
    18961 acatatgcac gacgctgatc atcaatttca ttcatacgga tgccatcaat tctgcccgct
    19021 ttcttctcgg ctgcagtcca gccccagaga tgatcgttga taaatttctg ggaagacctg
    19081 atgacccgct ctgcttccac atcttcgagc gctgcttcat agctaccaaa cgtagccctg
    19141 actgatgccg cttttttgat gttctcccgg gcgttcctga taacctgcgc agggttatcc
    19201 atgccgatga tgccgaacgc aacctggaaa ggttcgtaac cattcgccag cagataacgc
    19261 gaatagcgtt cctgagcctc ttttggggaa attttaattt tcaccagcgc agcctcagca
    19321 gcatccagat gtgcgggttc gttcagacgg ataacctcca gcacccaaag ataagcatca
    19381 gtctgcttat gcccggtgat tctccgttgc tctggcaggg gcttgatgtt tgcgagggcg
    19441 gagctgtacg ctgccgtcgg gatggtgaat agtactttat gttcgttgtt atcagtacgc
    19501 attacgcaac cgcctttttc ttatggaaaa ccagctcccg aacctgatca ccgttcatga
    19561 gcatattgtt gaaatcatcg tgatccggcc agtacacgct cacgcgctgc aggtcattct
    19621 ttgccatcag attggcatga gcacattcgc aagccgcagc cagcccggtg gcgctgttct
    19681 cgtcacggtc agcaaaaata atcagatgca gaacaccagc tggtacacgg aactttttca
    19741 taaagccgct gttaatggtt gcccaggtgt tcacgttata aatctggtgc gctgacagcg
    19801 ctgtttcgat gccttcggcg atacccagag tgctggcgac aggaaacatg cggatagcta
    19861 cagaacgagc gtgatccaga tagttatctt cctgcaggga tttgaggcgc tttgcactgc
    19921 taccgatatc tgctttttta tcaccatcaa gcagggtctg atgcagatag cacagctccc
    19981 ctttatcgtc cgtagcaagt gaataaagag actggaacac actcccgttg tgtctctgcc
    20041 tggcattgaa cctgatcgcc tcagcaggaa gactgaatat tccacgagaa ttaagatacg
    20101 cggcgccgga tgtaccacgc agtgcctcca gttttgaaaa cttgctcaat acccgtttgc
    20161 gtaagctggt ggcgctgctg gttaccggga ttttaacccg ttggtaatca ttaccgagca
    20221 ggcggtctat ttcggtacaa atctcgttaa atggcttcgc ctgtgtcagg gtgacaagtt
    20281 tcataccatc gccactacca catacacaga tccacgttcc tgcaccgtcg cggtcgtcaa
    20341 ttcggaactt gccacgtgca ccgcataccg ggcattcacc cttaaagtga ttttttccgg
    20401 ttatcggcgg cagaccgaag tgctctaata tttcaggcca acggcctttc gctgcatctg
    20461 ctgttttcat cttactgact cagactgttt atatttttct ggaattgttg ctttgcctgc
    20521 ataatgtgcc aagcctcact ttcttccggt ggaacatacc ggccaccaac aaccagtggc
    20581 agagaaagca cttcggatga gtttttcgtc atcctccgtg tccgttcacg acctttagca
    20641 aaccggatca gcttgtgtct gatgtagtta cttacctctg ggcttatttc catcgggaaa
    20701 ctgctcaacc catcaggcca ttcaccgaat ttctccctga aagtgtgagc gcaccatcca
    20761 tcactgactg cacgccccag tgaggcacgc tggcgctgat aaaatttgat ctgactccac
    20821 caggcctgtt tctctgcctt cgtggactga tgctgatttt tacccagctt attaagtttg
    20881 cggctagtgt cagtatcaac gtcttcacct cgcagcggct tgtgtccaca tttcgggcaa
    20941 acatagacgc cagctggctt catgtagtgg cattgagggc attcatgtgg caatttttcg
    21001 gcccgttcct caactgcgcg gcgcgtgctt tcctccatgc cgtcagactt accgggaaga
    21061 tcgtcgtact cgattgaatc cggataaccc agacggtgca cggtgccgct gtgatcgaag
    21121 ataaggcagg actctttacc cggtgcggtg cgaagtccac gcccgagtgc ctgcaaccag
    21181 cgaatttcgc tttttgttgg tctggcgtag atgatgcaac gaacgtcact atcgaatccg
    21241 gctaccagaa cgcccacact aacgatgatt ttcgttgcac cggtttcaaa gcggtgaatg
    21301 atggtctggc gctcatctac cggagtgtcg gcggtcatta cctcagcgtt aacagccgcc
    21361 aggttaaact ggattgtcag ataattggcg tgagctacgt tgacgcagaa agcgatggta
    21421 ggtagatccc gaccattctc cagccagttc tgtacgatgt cgcccaccag cgtagagccg
    21481 cacatgattt cagccagctg tgtttcgttg taatcgcggc cgtactcaag cgaagatgtg
    21541 gttttaacac ctttcagatc cggcttagtt ggcgcgtaaa attcgtattt actcagatcg
    21601 ccacgctgga ttaactcgcc gatggtggtc ggcttaatca gtcggtcata gtatttgccc
    21661 aggaacggag aaaacggagt acccgacagg ccaatcacct ttacgccttt gccgcgcaga
    21721 cgttcgatat ccttcaggat gcgtttttta cgcaggtgcg cttcgtcgat aatcagcaga
    21781 tcgatatttt caggaaaaac acgacgaata agcgtgtcag cgctggcaat ctgaattttc
    21841 cggtccggat cgtagttagg gtgatccgcc cagatataac cgatttcatc tccaggtaaa
    21901 ccatactgca cgaaccgatt agccgtctga ccaatcagga tggtgtacgg aacacagaac
    21961 agaacgcgca taccacggct gacaaaacca gcaactatga aggctgccag acccgtttta
    22021 ccgctacctg ttggcgagta aaccatgaag gtgtcgtttg ccttccagtc acggcgcaac
    22081 atgtttagcg ctcgctcctg tgcaaaattc ggcgtgattg tcagctgcat tgtgctgcct
    22141 ccccatggtt cagcgggagg cgcctgaaat ggtgaccgcg atgagacttt gttcttccag
    22201 caataacagc ataaacacta ccgtgatcaa acccggcact aattagcgcc tttttcccgc
    22261 aaaaggtttg ctcttccctg gtcgcaatat ttgtggcgac aatcaggccc ttaaattgtt
    22321 tgttgttcgc cccctgagaa ttattcagcc cggtgtgaat agaatgctga atgttttcgc
    22381 tatggctaac ccactccaga ttggatgcat ggttattcag cttgttgccg tccttatggt
    22441 tcacttcagg gtggttgtaa gggttgctgc aaaaagccag cgctaccaga cggtgtacgg
    22501 caaaagtttg gcgattacgt ttgtagctca ggttaacggt tttatatcca tcccggcgca
    22561 ctcttccctt gaggaacacg ccacgaattt ggcgaataaa aaattccgat tcagcaattc
    22621 gattaacccc acggatgcgt cccgccgagc taacgaggta agcatcacca tacggagcaa
    22681 caggaaccgg aagccatttt tcaccatcaa aactgttaac cataaaatca cctctgaaca
    22741 tttacatctg gacgtctagg cgtccgcaca tcttctggta tttccccttt acagagatct
    22801 acctaaccta tgtacccgtc ttcttggaaa agcctgttcc agcgcttcgc gctaaaagat
    22861 caacaccgcc ccctttcccc cataaacatt tagggggtgg taaatacatc cagacatata
    22921 gacgtctaaa tatttttacc attttcaatc agccaccgga cacctttaag cccggtacca
    22981 atcaggagcg cgattgcgtt cctgccaggg gcggctgagt tgtatacccc tgtaaagctc
    23041 tgccctgatt tctcacaaac aagcggagcc ttgtgtttgc ttcgtgcctt gctctgttct
    23101 ccttgcggaa tgaaacaggc tcagcgtcaa aagtgatttc gtatacctcc gcatatttca
    23161 gggcgacctt ccgtctcagt gacggaggca gcctctgtaa ctgctgctga atccactctt
    23221 cgtctgcctg gcagtaccta gatggcatct ctgtctgaac gtaattctgg gacatacaag
    23281 cccttcacct cccccgtgcg cgctaagctt ggatgtggaa aaagctctgg taggtctgga
    23341 cgaaattcat acgcttggat ctttccgtcg acagctgcaa ccaataacgg cacaaattct
    23401 ggtgaaatct tgttcttgca gttcaaccaa tcgcagactg ttgactgggc ctttccgcat
    23461 cgcttggcta aggcttgctg acttccaaga atctcaatag ctttctctat ggctttatgt
    23521 ttcataatcg catctcctat tgaacgcata ataacaatag gaaaagcgat attcaacgtt
    23581 aaacctagaa tatttatcgg aaagccgata aactagccaa gatagagagg ggaggaaaat
    23641 cgcaatgaca ttttcagaac gacttgattt agctatgcgc aacgcaaaat ttacgcaagg
    23701 taggctggcc aaagaagtgg gtatggctca gtccagtgtt aaccaactac ttaacaaagc
    23761 caacggttcc agaaaaacag tcgaaatagc aaaagttttg ggcgttaacc cggaatggct
    23821 cgcatcgggt gttggcccaa tggaaatcgt gacctctgca gactcacacc aaatacgtaa
    23881 catttctgaa gattgggtta ctgattcgta tgtcgtggat gttttagaca tcagatatag
    23941 ttgcggacct ggtagttata actctgattt tcctgacatt gtcagatcta tagcgataga
    24001 gccaggatac gcatcaagag tttttggcgg cagaccagca tcagcaatca aagcaataaa
    24061 cgcccacggc gacagtatga aaggcacaat agaccctgaa gacttggtat ttgtagatgt
    24121 atcagttcgc acatttaatg gtgatggtat atacgccttt acatactctg gaacatcgca
    24181 catcaaaaga cttcaaaaaa tcaaagatac tctaacggtg atatctgaca acccagctta
    24241 caaggattgg gctatcgaac cggaagactt cgaacaactt cacattgacg gaaaagtaat
    24301 tgtcagttgg cctatgacac tacaccgttt tgcataagca aaaatcattt aacccgccca
    24361 ctcttaagtg ggcttttttt tggaaaaaat cgataaaccg attgacaggt gaaatcggta
    24421 aacctattat cttgacatag gtatcgaact caccaaagca attatagagg atgagaaaaa
    24481 tgtcatcgta tatggggtta ccaacaaatc agcacgatgc tcttgagaat attcagctct
    24541 tagtaggggc tggtgcacta ctgtatagca gcaataaccc tgagttaaat gaacttgcca
    24601 gctccatcct ggctgtaact caaaaatata gcctcaccgc gtcacgttta tgcaaacaaa
    24661 atttagctat taattcccag aatcggattc gcacccaacg cgaagactgc ggcttaacaa
    24721 cagccgaact cgccagactg ctcgacctcg atgaagaaat catcatccag tgggaaagcg
    24781 gagagtacga accaaccatc agcatgctta tccctctggc taatgttctt ggctgtgatc
    24841 caatgttgtt attgactgat gcttataaac aaaatcagga ggaagcataa gatgaaaatg
    24901 ttcaaaggcc ttaccaacga accggagaca gcttttcatc acattgccgt actgcttgaa
    24961 gcggggttaa tcatttcggc ttccggtgat gatgaatgtg atgaactttc ggatgatatc
    25021 tttttactgg cacaacaata cgccagaagc gcatgcgatg catttaagga gcaaagaaca
    25081 tgaaaacgcc catgactatg ctcgaatatg tagccgcaga aataaaagaa aacacgtcaa
    25141 tgcttgagtt tatctttaaa aattcgcccg actcaggaga gttagacaat tatttatgtt
    25201 gtctcatcag gtccatgaat aagacctgtg aaatggctta cgagtatata gaaacactac
    25261 gtaacgaata aagaatactc aataaaaaat cacacataaa aacatgacgg cctctggtcg
    25321 ggagttttca caaccagaaa ataatggtga tacatgaaaa atagaagcgc ttataaaacc
    25381 gccttattaa tggctaatgc aggatactgg tcagttgcaa tactgtttct taggaaagca
    25441 tacgggaaat aacaaatggc atatgaaatc attggtaaat atcgtcaccg catagtcaca
    25501 acagcattac agcggatgca acgtaaaaca ggaggaaatc ttctcatcgt aaatcttcct
    25561 gacggagggt taactaccat cgaaattacg gaacacttca tgacacaact gttattacga
    25621 tttgaaggtc ttacccgtgt tgaatttgga cggacggatg gcgagaccgc aatacttgcc
    25681 gcataccgga atgccatcaa tattaatcaa cacactgagt atctaactga atcggggaag
    25741 ttaattgtag acaacctttt aaaagaggtt gttgattacg aaaaagaaaa atataccagt
    25801 ggaggaatta actgatgtct gatttatccc ctcttataca cgaaaaagta cagctcatta
    25861 cgacgattga aaatggtcag gtaataaata cccgtaaagt tcacaataac gggatgattg
    25921 ccagcatgga taccttctta tggatagcaa agaaagcggg atatcaggta atcgctcctg
    25981 atctggagga aatcggtgac attaacagca atacgaattc ctgagtgggt gcacctgcag
    26041 gcggtccgtg ttctccgcca gttcagagcc agacgaattc atccctgtcg tatgcacggc
    26101 tccggaaacc tgagtctgag ggttaatcgc cgctggcggc tgctgtcccg agacggcggc
    26161 cagaactggg aagtaatgag ccatgaacga tacagcaaag ttaaggaccg gaaatgaaca
    26221 ataaaccttc aaaccaaaag gagagtattg atgattaatt caaccattac ccctgaacct
    26281 acattaacag gcatccgttt tggtaatcga attattggtt attcaactgc ggttcgccag
    26341 atcgacaacg gtaactatga caaacgaatt ccggacgggc tcgatctgat agcttgcatt
    26401 atggaaggga ttgaaagcgg ctggtttatg ccgtgtattg aaaaacaaat catcctatgg
    26461 cgctggcttg ttgtcgccgt gttcattgct gaggagcagg agaagaacgg gactgtcgat
    26521 gttcagaatg acaagggcgg cgtagataca gccgttgtat acacgggaaa acccggttca
    26581 atcagcattt atccgtggtc tgagcgcttc gcgctcgcta accatatcga ggcgggcgca
    26641 attgaaaaat acgggcctga cgttggtcag cagatggcgc tgcgtatgta tcaggacatg
    26701 gttgtaactg acgagaaatg cggattcagg ctgtcagcga tgggccggga gggactcaac
    26761 atactacatg acagattcat tgaactgatt cagaaagacg gtatgccaga catgccggtt
    26821 atgcactgag gtggacgaaa atgaacatag taacgatcaa taacaaacag cttccggcag
    26881 tcgaatatcg cggtcagcgt gttgtgacgc tggcgatgat tgatgaagtc caccagcgac
    26941 cggaaggaac cgcgcgtgca gcattcaacc gaaaccgtga gcatttcatc agcggtgtgg
    27001 attatgccga attaggtgcg gacgtaatac gtacggacct cccggaagga acattctcta
    27061 aatttgcacc atcagggatt gtactttttg aatcaggtta cctgatgttg acgaagccat
    27121 tcaatgacgc ccttgcctgg caggttcagc gcgaactggt taacagctac ttccgcactc
    27181 gcacaccgct aacggaaatc gagatgatcg ctgcgatagc cgccgacgct gttcgtcaac
    27241 agaagcgcct gaatcatgtg gaagaacagc tcgaaacagt cacagaagcg gtggaaacca
    27301 tcaaacgcgg aaatatgcgc gccggatatg tcggttatcg ccaggtggtc gccaaaagcg
    27361 gaatgactga tgctaagtgc cgcaatctgg tcaatgccta cagcatccca accgatacgc
    27421 acgaatttat gacaccagat ggtcttcttt cgcgccgggc tatcgtggag ctggagccgt
    27481 tcatgaaagc atttcatcag atgatgtcag aggcagagcc acgcggtacc cgctggtatc
    27541 acccgaaaat ggggctgttt caggtgattg gttgggaggg taaataatgc cactcattac
    27601 acaaaatttc cgcctcaatg cgctggcaaa tcagtatgcg gctgcactgc acaaccacat
    27661 atccgccacc aacggagatc atttcatgat taatgtcaat gggcaatcca tacgagtaaa
    27721 catcatcggt ggggttaaag gtatcaggga tttaatcgat ggttatgctc tgaaggctat
    27781 caaagaaaat tacccccaat gggaagccgt tggtattcag ctgttaagca aatgcgttcg
    27841 tggaaacgag ctgacgcaaa gcggcaaaga aatctggcag agcatggtta acgacatgag
    27901 cgataccgtt atggggaaca tgtaaatgaa aatccaatac aaggactatg gcgccgtagc
    27961 gaacatcgtg atcaccagtt cctgcctgac attgcaaaag cataaccgcg ttgttgatgc
    28021 ggtgctgttt aatgtacagg ggatctatga aaaccgcagc ggctttttct gggttaagtc
    28081 atcattgtca ggaaaaacac gcgacatcct gcgcgcctac aaaatcgtac agcgggaggc
    28141 aaagcgatga agtcattcct cctgtccatg ctgtttggtc tgctgctggc gtccgtcgtt
    28201 ttcggtgcgc tgattgaata taaattttta atgaattact gaggtgcgga atgagcaaga
    28261 tcaaaaaccc agttgtactt atccataaac gagaaaatag tgactcctac gtcgttgcaa
    28321 tcacctgcgg tagccagaac tatcacgacg ccgttctgat ggcgtcgatg gaactggata
    28381 tgagtgacga tgatgtcgat acctggagta aaaccggcta ctacatggct caagaaatcg
    28441 agcgacttaa acaagcgctg tccgctgcgg aaaataattt gattgattcc gaatgcctcg
    28501 tcgctgaatt aagcaaagca aagaatcacg catatgggct ggtcgacaca tacgactggc
    28561 aacgtcagcg tctccacgca gccgcagaga aagtcatcaa atggtgcaga caagaagctg
    28621 aacaccgtac cggcgaccca gagaaagcag aaaactatgc gtgtgttaaa gaactgcgcg
    28681 acgcattaac tttttgcaaa agttctgaag gcagcaagaa gaaaggcctg accatcaccc
    28741 tgcccgatat cacatcaaag gcattctgga gcggtaccgg aaaaaacgca aaattccatc
    28801 cggaaaccta taaacgctgg gtgaaagaag ctatcaaacg ggattgtgtt attgccggga
    28861 tcggcgtgga ggtgaaatga tgaattggcc tgaagcattc accgcagtag gagttgcaat
    28921 agcggtggca tttattctgt attcgctttt ccgctgggga taaaggaatg ttcgctctga
    28981 ttcaacgtgg tcagatatac accgatagtg ctggctatcc ggtaaaaatt gttcgctgca
    29041 taaacaacac tgtgttgtac agaagaatgg atgggcgaac acagtcagta aaaataaacg
    29101 atttcaatga actgtttgaa cggattgatc accaggaata cagacaaatt ctggcagaaa
    29161 cagagcagga agctcatctg aaaaaattac gggccatgaa aaggaagtaa agaatgaata
    29221 aagcatttga actatgggtg cgccagcgtt atggcaatcg ctatgacctg acgcgagatg
    29281 ttgacggctt ctactgtcgt gaagttgtga agcgaatgtt tgatgtgtgg tgccactgcc
    29341 gtggatgaaa attttatgag gttggcatgc agacaatcat ctatcagata acccccagca
    29401 aatggtgtac ggagagagtc ctcattgcat caacagggct aaagcctggc accattgagc
    29461 gggcaagaag aaagtcatgg atgcagggaa aagaataccg ccattacgct gtagaaggtg
    29521 atccggggca ctacagtgaa tgcctgtaca acatcgaaga aattatgcga tggatcgaaa
    29581 accagaaaca accaggtgcc aaaaatgcaa gttccggtta acctgttaat gctcctggac
    29641 gtctgggagg tttaatgagt aacgcatcat acccgacagg cgttgaaaac catggaggat
    29701 cactccgtat atggtttcac tataatggca aacgtgtcag agaaaacctc ggtgttcctg
    29761 acacagccaa aaaccggaag atcgctggtg aacttcgcac ttccgtttgt tttgcaatca
    29821 gaatggggag tttcgactac gccgcgcagt tccctaattc ccctaacctg aaacactttg
    29881 gtctgggaaa aagagagata accgttaagg cactttcgga aaaatggttg gaccttaaga
    29941 aaattgagat ttgtgcgaat gcacttaacc gttaccagtc agtaattaaa aacatgttac
    30001 caatgttagg tgaaaaaaaa ctggtttcat ccataacaaa agaggattta cttttcgtaa
    30061 ggagagattt gttgaccggt taccaaaagc tttctaatgg aaagacttct tccataaaag
    30121 ggcgctcagt ggtcacggta aactactata tgacaaccat agctggaatg tttcaatttg
    30181 caacagataa tggttatacc tcaggaaacc catttaacgg tctggctccc ttaaaaaagt
    30241 ccaaggtaaa accagatcct ctcacccgtg acgaatttat tcgttttatt gaggcttgcc
    30301 gtcatcaaca aacaaaaaac ctgtggattc tcgctgtata cacgggtatt cgtcacgggg
    30361 agttggtatc gctggcatgg gaagatatag accttaaagc aaggactata accatccgta
    30421 ggaattatac aaaacttggc gaattcactc caccaaaaac cgatgctggc accggaagga
    30481 caattcatct ggttcaacca gctattgatg ctcttaaaag tcaggcggaa atgaccatgc
    30541 ttggaaagca acattctgta gaggtaaagc agagggaata tgggagaagt actgtgcata
    30601 aatgcacttt tgtttttagt cctcaggtaa taaaacagcg gcagttttcc ggaccgcact
    30661 ataaggttga ctccatcagg gagtcatgga caagtatctt aaaacgcgca ggtctgagac
    30721 acagaaaatc gtaccaatcc aggcatactt atgcatgctg gtcacttgcc gctggagcta
    30781 atcctagttt tatcgcaagc cagatgggcc acacaaacgc acaaatggta ttcaatgttt
    30841 acggagcatg gatgaaagac aacaatcacg aacagataga actccttaac aaaagactat
    30901 ctgaaagtgt cccatgtatg ccccataaga aagtggggta aaataaaaac ttgtaaaatc
    30961 agttagttta cccttaatcc ctgtcacgtt acgcgcgtgg cagaggcgtt acggattgct
    31021 gaaaccgcaa cggacagacg gcggtcatcg gctgttcaac gatgccgata ttgaccggat
    31081 ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct
    31141 cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac
    31201 ttacctgcaa agcggcaatc tacatagcct gcgaacgtgg atcaaagagc gcggtcagga
    31261 ttaccccgcc cagacactca ccacatatct gtttattcct ctgcgccgac ggcttcagtg
    31321 ccaacaaccg actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat
    31381 cgccatttgt ctggcttcgg cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg
    31441 gaatattcag gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggatg
    31501 gcgcattgat gtcctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggccg
    31561 tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag
    31621 ttggcaagaa caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg
    31681 cgctttactg tacaatcctt tcgttaacat aaggagtgca ttatgcgcat agctaaaatt
    31741 ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca
    31801 ggttatacct ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt
    31861 ctgcgatgat cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa
    31921 atccgctaag cccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga
    31981 tcgtcgccgt accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag
    32041 ccgccagcgg tagctcgacc ttacgcagtc gctgaccacg actcattccc atacctttcg
    32101 caacttctgt cacgctggca tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg
    32161 gcagcacacc gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg
    32221 gaacggcgat cgccagcact gcgacgggcg gaaaagtctg tccaacggcg gcaatagttt
    32281 ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac agcaattccg gcaccagtgc
    32341 caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag
    32401 ctgcaaaact ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca
    32461 gcggctggct gtaaggcagc cagaaaatca gcgccacaaa cagagcaatg agccagaaca
    32521 gcggatcgcg caacatcttc atacgcttac gcctccacca gcagatcctg aaaatgcagc
    32581 gtgccgcaag gctggccctg catgttcacc accggcagca cctcgcatcc ccgcgcaaca
    32641 aacagagaga gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc atctgctcgt
    32701 tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa gcaggcgcac acccagttca
    32761 ctacgtccaa aaaactggcg gacaaaatca ttcgccggac gagtcagcat cgtcagcgga
    32821 ttgccctgct gcactacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc
    32881 gcctcatcaa tatcatgagt gaccagcaca atggtacgcc ccagcaaacg gtgaatgcgc
    32941 gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc
    33001 attagtaaga cttgcggatc ggcagccagt gcacgcgcca ctcccacacg ttgctgctga
    33061 ccaccggaaa gctgatgcgg ataacgctca cgcaaatttg actccagccc cagtagcgcc
    33121 attaattcgt cgatacgatc gtcaatccgc gcccgcgacc atttttgtaa ttgcggcacg
    33181 gtagcgatgt tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag
    33241 cccatccggc ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg
    33301 atctctccgc tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg
    33361 gagccagatg tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg
    33421 ttaacggctt tttgtgcgcc gaacagtttg ctgacatggc taaattcaat cattacgttt
    33481 caccttcagc agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat
    33541 taccgggatc acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac
    33601 cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg
    33661 caccatcacc acccgcaggc tgcgcagaaa taccggtaac gccagcggta actgaacatg
    33721 caggaaacgc tgcgccccgc tcatccccat cgctctggcg ctttccagca catcgcgcgg
    33781 gatctggttc aaaccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag
    33841 cgcaatcagt gcgggtgtca ttccggttcc tgctatgccg agcgtcccca gccacggaaa
    33901 ggccgtaacc agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac
    33961 ggtctgaatc acattgagca gagaaaaaat tgccccctgc cgagcagtgg aaaagtagca
    34021 ccagatgccc aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt
    34081 cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag
    34141 tgagagatcg ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat
    34201 atgcaacaac cagcgccaca gcggatgcgt ggagattcgg cggatggcat cgctacaggc
    34261 cagcaatgcc agcgccgcag ccagccagaa accactgccg aggctggtac gcgccagcgc
    34321 actgccattt tgcgccagtt gggtcgccgc ctttccagct ccccacacca gcaatacgaa
    34381 gacgaattgc gccagaatga gtgcacaaat gctgcctttt ttaccgggaa taaaacaggc
    34441 cgtcagccag gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagctgcca
    34501 gagatgacgc ccctcacccg aaactaaacg attaggcgcg taactgataa acggcagcgc
    34561 cgctgcgatt gccgtcagca acagcagcag cgccagaaca ggattaatac ggaaataagt
    34621 cacgggaaat tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc
    34681 atccagtcct tccacagcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct
    34741 ggcgaagact ggctgtagcc actgtgccat ttgcggatac tccctcaaca ccgactcacg
    34801 caccactggt gcaggcgcgt agataggttg cacaccttgc ggatcgctta aggtttgcag
    34861 ccccagcgcc gctaccgggc cgtcagtgcc gtaagccatt gcagcattaa cgccagaggt
    34921 ttgctgggca gcggctttga tcgtcaccgc cgtgtcgcca ccagccagtg acagcaactg
    34981 atcctgaccg agcttaaagc cataggcttt ttcaaacgcg ggtaacgcat cggcgcgttc
    35041 gataaactct gccgaggctg ccagtttgaa ggtgccgccc tcttgcagat aacgactcag
    35101 gtcggcaagc gaagtgagtt tatttttctc tgccacatcc tgacgcacgg cgatagtcca
    35161 ggtgttattt gcaggcgcgg gcgtcagcca gattaacttg ttgtgctccg aatcgagctt
    35221 tttgactttc tcgtaacctt gctgcgcgtt tttccacgct gcatcgtttt catctttaaa
    35281 gaagaaagcg ccattgccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc
    35341 cccgcgcacc acaggagtcg ttccgagttg cactttattc accgtcggta cgccgtggct
    35401 ttccagcacc tgcaaaatga tattgccgag tagcgcacct tcggtatcga tttttgaacc
    35461 gactttaacg ggggaagccg cctgtagcgg caggctcacg gctgccaaca taaccagtga
    35521 acctgcccag agctttaaga gtggcatgat gctttcctca ttcttttact gttgttttca
    35581 gcgaattaag agaaaagcat agttgataat ggcggggtta gcttgagcgg aaggattcag
    35641 ttgcagaatc agataaatac ttaagaggca tattcggtcc ggcgtttcct gccggatgcg
    35701 gcgcgagcgc cttatccggc ctacaaaggg cgcaaacgtc gcgcccttac taaagcatta
    35761 cagcaactca aactcgcctt tcttaacgcg tgcggaatca gtgccgataa agacattgaa
    35821 cttgccaggc tcggcgtcat atttcatctg ttgattccag aacttcagcg cctcaatatc
    35881 gatcgggaag ctgacagtct gagtttcgcc cggtttcagg gtgattttct caaagccttt
    35941 cagctgtttc acagggcgac tcatggaagc cgtcacatcc tgcaagtaca tctgcactac
    36001 cgtggcaccc tcgcgcttac cggtgttcgt cacctgcacg ctggcagtca ctttgccgtc
    36061 acgcttcatg gtcggcgcag aaagtttcac atcagagacg gtgaaagtgg tgtagctcag
    36121 cccatagccg aacggataca acgccccgtt agcttcatca aaataacgcg aagtgtattt
    36181 gttcggcttg tcggcattat acgggcgacc ggtattcaga tggctgtagt acaccgggat
    36241 ctgcccgaca gaacgcggga aggacattgg cagcttgccg gacgggttgt aatcgccaaa
    36301 caatacatcg gcaattgcat taccgccttc agtccccgca aaccaggttt ccagaatcgc
    36361 atcagcctgc tgatcttctt tcaccagcgc cagcggacgc ccgttcatca gcaccagcac
    36421 cagcggttta ccggtggctt tcagcgccgc aatcaagtca cgttggcttt gcggaatagt
    36481 gatatcggtc cggctggagg cttcgtgcgc catcccctgt gcttcaccga ctacagccac
    36541 caccacatca gattgtttcg ccgtctgcac cgcttcatca atcatctctt gcggcgaacg
    36601 cggatcgact ttgaccgctt cttcatactg attcaggaaa tcgataatgc ctttgtcact
    36661 ggtaacgttc gcccctttgg catacagcac tttaccgttt tcaccgacgg catttttaat
    36721 cccggtcagt acggtcacgg attgatcggc aacaccggct gcggaccagc tgcccatcac
    36781 gtcacgttta ctgtccgcca gtggcccaac caccgcaatg gtggccgatt ttttcagcgg
    36841 taacgtttcg agacggtttt tcagcaacac caagctttcg cgcgccactt cacgcgcttc
    36901 tttacggtgc aggcggcttt cggcattggt atccaccggg tcagactctt tcggccccaa
    36961 atggctgtat gggtcgttaa acaaccccat atcatattta acgttcagta catggcgggc
    37021 agcatcgtcc agctctgcca tcgtcacttt gccggattta atcaacccag gcagatactt
    37081 cgagtagtac tcgtcgctca tgctcatgtt gattccggat ttcagcgcca cgcgcaccgc
    37141 atcttccggg tctgccgccg tgccatgttt aatcagctct ttgattgcac cgtgatcgga
    37201 aacggtgatg cctttaaagc cccactggtc gcgcagaaca tctttcagca gccaggaatc
    37261 ggaggtggct ggcgtgccgt tcagcgagtt cagcgccacc atcaccgcgc cgctgcctgc
    37321 gtccagcccc gctttgtacg gcggcatata atcattaaac aggcgctgcg gactcatatc
    37381 gacggtgttg tactctttac cgccttctac cgcgccgtat gcggcaaagt gtttgacgct
    37441 ggtcatcacc gagtagcgat ctgccgggct tttaccctgc atcgcttcca ccatggtttt
    37501 acccattgtt gaggtgagat acgtatcttc gccaaaacct tcggaagcac gtccccagcg
    37561 cggatcgcgc gagacatcga ccatcggtgc ccaggtcata ttcaggccat catctgccgc
    37621 ttcataagca gagacacgtc cgaccgtttt cactgcatcg aggttaaaag acgaggccag
    37681 accgaggcta atcgggaaca ccgtgcgctg accgtgcagc acgtcgtaag caaagaaaag
    37741 aggaattttc aggcggctta attccatcac ctgatcctgc atggcgcgga tatcctgacg
    37801 ggttacggtg ttgaaaatcg ccccaacctg accgtctttg atcatctcgc ggatcgcctc
    37861 tttcgggtta tccgggccga cgctgattaa gcgcagctga ccaattttct catcaactgt
    37921 cattttctta agcagttcgg tgacgaacgc atcccgcgct tcgggcgtta atggatggtt
    37981 gccgaataaa tcatccgcca gtgctggctg cagggccaga ctcaccgcga ttcctactga
    38041 acatagccat ttcatatgga tttttcttcc tttgttgccc gacgtggcag cgaaaatggt
    38101 gcaaaaaccg tagtttgcca taagcatgat ggagagagaa aaagaatgct cagtttattg
    38161 tctgaatttt caaaatattc actcgctgaa ttgttataca aggcgctatt ctagtttgtg
    38221 atattttttc gccaccacaa ggagtggaaa atgtcttcca tgacaacaac tgataataaa
    38281 gcctttttga atgaacttgc tcgtctggtg ggttcttcac acctgctcac cgatcccgca
    38341 aaaacggccc gctatcgcaa gggcttccgt tctggtcagg gcgacgcgct ggctgtcgtt
    38401 ttccctggct cactactaga attgtggcgg gtgctgagag cctgcgtcac cgccgacaaa
    38461 attattctga tgcaggccgc caatacaggc ctgaccgaag gatcgacgcc aaacggtaac
    38521 gattatgatc gcgatgtcgt tatcatcagc accctgcgtc tcgacaagct gcacgttctt
    38581 ggcaagggcg aacaggtgct ggcctatccg ggcaccacgc tctattcgct ggaaaaagcc
    38641 ctcaaaccgc tgggacgcga accgcactca gtgattggat catcgtgtat aggcgcatcg
    38701 gtcatcggcg gtatttgtaa caactccggc ggctcgctgg tgcaacgtgg cccggcgtat
    38761 accgaaatgt cgttattcgc gcgtataaat gaagacggca aactgacgct ggtgaaccat
    38821 ctggggattg atctgggcga aacgccggag cagatcctta gcaagctgga tgatgatcgc
    38881 atcaaagatg acgatgtgcg tcacgatggt cgtcacgccc acgattatga ctatgtccac
    38941 cgcgttcgtg atattgaagc cgacacgccc gcacgttata acgccgatcc tgatcggtta
    39001 tttgaatctt ctggttgcgc cgggaagctg gcggtctttg cagtacgtct tgataccttc
    39061 gaagcggaaa aaaatcagca ggtgttttat atcggcacca accagccgga agtgctgacc
    39121 gaaatccgcc gtcatattct ggctaacttc gaaaatctgc cggttgccgg ggaatatatg
    39181 caccgggata tctacgatat tgcggaaaaa tacggcaaag acaccttcct gatgattgat
    39241 aagttaggca ccgacaagat gccgttcttc tttaatctca agggacgcac cgatgcgatg
    39301 ctggagaaag tgaaattctt ccgtccgcat tttactgacc gtgcgatgca aaaattcggt
    39361 cacctgttcc ccagccattt accgccgcgc atgaaaaact ggcgcgataa atacgagcat
    39421 catctgctgt taaaaatggc gggcgatggc gtgggcgaag ccaaatcgtg gctggtggat
    39481 tatttcaaac aggccgaagg cgatttcttt gtctgtacgc cggaggaagg cagcaaagcg
    39541 tttttacacc gtttcgccgc tgcgggcgca gcaattcgtt atcaggcggt gcattccgat
    39601 gaagtcgaag acattctggc gttggatatc gctctgcggc gtaacgacac cgagtggtat
    39661 gagcatttac cgccggagat cgacagccag ctggtgcaca agctctatta cggccatttt
    39721 atgtgctatg tcttccatca ggattacata gtgaaaaaag gcgtggatgt gcatgcgtta
    39781 aaagaacaga tgctggaact gctacagcag cgcggcgcgc agtaccctgc cgagcataac
    39841 gtcggtcatt tgtataaagc accggagacg ttgcagaagt tctatcgcga gaacgatccg
    39901 accaacagca tgaatccggg gatcggtaaa accagtaaac ggaaaaactg gcaggaagtg
    39961 gagtaaaaat tacggatggc agagtatcgc catccgaatt cacttaatcg ttctgtgccg
    40021 tctgccccgc agccgccatt tgggcggctt tttgtttttt atagctcaac gctgctgccg
    40081 gcacaggcat cactttaccg gtttcaatcc aggtacgcag gcggctggca tcggcaaaat
    40141 gggtatattt gccaaacgcg tccatcacta ccagcgccac cggtttatta ttgataaccg
    40201 tacgcatcac cagacaatgg cccgccgcat tggtaaagcc ggttttggtt aactgaatat
    40261 tccagttatc gcgatacacc agatgattag tattgcggaa cggcagcgta tacgtcggat
    40321 tagagaaggt tgccatatct tcccgggtag tacttaactg cccgatcaac ggatattgtt
    40381 tgctggcaat gagcagtttg gttaagtcac gggcagttga aacgttatgc accgacaatc
    40441 cggtaggttc aacaaagcgc gtgttgttca ttccgagcga tttcgctttc gcattcattg
    40501 ccttaataaa ggctttgtaa ccaccgggat aatggtgcgc aaggcttgcc gccgcgcggt
    40561 tttctgaaga catcagcgcc agcaacagca tatctttacg gctgatttcg ctattcagtc
    40621 gtacgcgcga atagacccct ttcatctccg gcgtctggct gatatccact tttagttttt
    40681 catccagcgg cagtcgtgca tccagcacaa ccatcgcggt cattaatttg ctgatagacg
    40741 caatcggacg caccagatcc gggtggttcg aatagatcac tttgttggta ttcagatcaa
    40801 caatcatcgc gctaccggag gcaatttccg gttgtgaagc ggtggtagcg gctgccgttt
    40861 tcgcaacggc ctgcggtgca aaaggcacag ccagcatcag ggccaggcta aataaagaaa
    40921 ctcgaaattt cggcatgatg agcattcaga tagtggttca cgcgcacggg ttgcgcaccg
    40981 ccggagtaag gatttactga ggctagcgac gccatcataa cgagcaaaaa gtgcgatcgt
    41041 caaaggagaa tcgtgaggaa atgctgcatt gctgacattt acgccagcaa tgcaacgtca
    41101 aagaactttc tagaacaaac gataaccgta gccccatagt ataacggtta gggcgagcag
    41161 cacttccagt accagcacgc caatcgccag cgtcgaacta gagaagctaa ggccttcctc
    41221 tttgttgata ttcaggaagc tcggaatacc aaggtaaagc aggtagccgg tgtaaaacag
    41281 cgccaccgtg ccgaccagcg cacacaacca gaccagtgga taaagcgcca ccagaccgct
    41341 taaaaacagc ggagttgcaa cgtagcccgc gaagaccatg cagtgcgcaa gtgacggacg
    41401 ctgcggataa ttacgcgcca tccaccagat gacccgcccc atcaccgcga ccccagccag
    41461 cataacgccg taaaacaaga cagccagcgc cagtccggta aaccaggata acttcaggat
    41521 agtgccatcg ccaaaattcc agccaatctg tgtagtgcca atgaaggcgc aaatcaccgg
    41581 aatcgccgcc atcagcaaaa cgtggtgggt gtaatggtga gaaatcgttt cgttttcgcg
    41641 attaatcacc tgcatttcac gatcgggatg ggaaaacagt ccccagacat ggctcatacc
    41701 gcccccttgt tgtgagttca tgaacctgac agttcaagta taagtcagct tgtgattatt
    41761 ttttgttcgc ccatgtaatt ttcactgtct gatgaacgtc ctttttcagc gtgaatgatt
    41821 cacagggtgt atgcttactg gcaaccaaag ggagacagac tggcctatgg atctcaatac
    41881 acttatctca caatatggtt atgccgcgct ggtgatcggt agcctggcgg aaggtgaaac
    41941 cgtgactttg ctgggaggcg ttgcggcgca tcaggggcta ttaaagttcc cgctggtggt
    42001 actttctgtg gcgcttggcg gcatgattgg cgaccaggtg ctctatctgt gcgggcggcg
    42061 gtttggcggc aagctgttac gccgtttctc gaaacatcag gataaaattg agcgggcgca
    42121 gaaacttatc caacgccatc cgtatctgtt tgtcattggt acgcgcttta tgtatggctt
    42181 tcgggtgatt ggcccgacgc tgattggtgc cagccagctg ccgccgaaaa tctttctgcc
    42241 gctgaatatt ctcggcgcat ttgcctgggc gttgattttt accactattg gttacgctgg
    42301 tggtcaggtg attgcgccgt ggttgcacaa tctcgaccag catttgaagc actgggtctg
    42361 gttgattctg gtcgtggttc tggtggtggg cgtgcgctgg tggctgaaac gacgcgggaa
    42421 gaaaaagccg gatcatcagg cgtaaaacca ttgccctgga taaggcgttc gcgccgcatc
    42481 cgacatccgg agtaccatgc ctgatacgac gcttgtgcgt cttttcatgc cgaccaaaat
    42541 attacccaat gaaatatcca cgcacctcac tgcgctattc tgggttgaac tgtggattcg
    42601 ccaacataaa gccgccatcc actatcaacg actgcccggt ggtgtaattt gcgccctccg
    42661 aacaaagcca caccaccagg ctggcaatct catgcgttgc gccaaaacgc cgcaagggaa
    42721 tcgaaggctc cgcgtcgggc ttcacgtcgc tgtcatccat gccattcatt ggcgtggcga
    42781 tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct gaccagctcc agcgccatcg
    42841 ctttggttaa cccaccgagc gcatgtttag cggctgtgta ggcgctggca tccggcagcg
    42901 gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc gccctgccct tgtttcacca
    42961 tctgacgagc cgcaatttgc gagcataaga atgcaccatc gacatcaacg gtaaaaatct
    43021 tgcgccactc atcaaaagcc atatcaagaa acggcgcttt ggtcattgca cccgcattat
    43081 tcaccagcac atcaatgcgc cccagccgtt gaatgagttt ctccagcgcc agtgcccctt
    43141 ctggtagatt gccgagatcc agctgcacga tctccgcacg tacgccgtgg ctaactacct
    43201 cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca ggtaatacca atatcaaacc
    43261 cctgctgcgc cagtaataac gcgcactctt tgccgatccc cgaatcggag gcggtaataa
    43321 tcgcaacctg tgccatcgag ttctccactt aacgctgaat aaacgttaag tatagaaggc
    43381 gcatatcatc agcgtttgta acccccgccc aacgcaccag tgagttgaat ggaggcatcc
    43441 agccactgcc cttgcaataa caggccattg gcccgctcac gcagcgcggg gattctggct
    43501 tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc gcgcctgcgc aagacctacc
    43561 acacgcaagg catcgcgctc aatttgcgcc tgatgctggt ttttctccgc cagtgtctga
    43621 acctgactgg ctgcccgcgc cacgtcattc accgcttcaa ccaccgcttt gttgtagctg
    43681 gcgatagaca agttgctttc ggcttttgcg atatcgagat tggcgttaag acgaccacta
    43741 tcgaaaatgg gtagcgtcag gcctgccgta acgcccattt gctgcgcgga atgacggaac
    43801 agatcgctta agtgcaacgc atcctgttgc aggaaggcca tcaggttgat gtcaggataa
    43861 aatgccgctt ttgccgcatc aatggtgctt agtgatgact caacgtacca gtgcgccgcc
    43921 tgcaaatctg cccgccgggc cagtaaggag taccccagtt catcaggaag ctggcttgcc
    43981 actttcggca acgcgaccgg tttaagcttc aatgactttg tctggttatt tgtaagtgcg
    44041 cttaaccgtg cctcaataat tttcattttc cccgcgacat cgttgagctg ctgccgggtt
    44101 ttgctggcat taatatcggt ttccacacct tcaactgaag aagtaatccc gttctgatat
    44161 agctggcgat cggtcgcgat aatggtgttc tgctcttttt ctatttgctg caagaccgtg
    44221 tttaacgccg cctgggtttg ccactcccag tacaggcggg ctacgctgcc agccagcaat
    44281 tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg tacccaggcg ggcagtaacc
    44341 tccgcccgat tctttcccca gatatcgaga tgccagcccg ccgttaagcc aaaagtaccg
    44401 ttggtgtacc acgggccggt cgtacctgcg gccggatcgt tcagagcaaa cggccccatt
    44461 aagccttctg ccgacatttt ttgccgctcc atatccgccg aaaagtcgat ctgcggacca
    44521 tcctgagtgg caactgcctt cgcctgggct tcagctagct gaatgcgctg ttcagccacc
    44581 tgcatatccg gtgcgttctg tagtgcattg ttaattaagg aagtgagttg attatcgtga
    44641 tactccagcc accattggct gtctggccaa ccatttttca gcgccgtggg taatgcggtg
    44701 tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc gggtttcatg cataggcgca
    44761 cacccggcca gcatcagtaa cagcggaaaa caggcgatgg ctggataaaa ggaatcacga
    44821 ttcatggggg aataatcagg taagaaaagg tgcgcggaga ttaccgtgtg ttgcgatata
    44881 ttttttagtt tcgcgtggca atacatcagt ggcaataaaa cgacatatcc agaaaaatat
    44941 acactaagtg aatgatatct tccgatttat cttaatcgtt tatggataac ggcaaagggc
    45001 ttcgtttttt cctatactta ttcagcactc acaaataaag gaacgccaat gaaaattata
    45061 ctctgggctg tattgattat tttcctgatt gggctactgg tggtgactgg cgtatttaag
    45121 atgatatttt aaaattaatt aatgtcatca ggtccgaaaa taacgagaat atttcagttt
    45181 ctcatcctgt tgcgctcctg tcatgtgcat tgcttcatat aatcactggc gcaaggagcg
    45241 cgcagggggc ggccaatcgc cgccgccccc tgcacccccg ggctctggcg aacaaaatcg
    45301 ccgctgcgcg gtgccctcgg cttatccctt acggctaccg ggtcgggcgc gaggtaacat
    45361 ccctgtaaaa cgcgccctca gcccacatcc atgtgggctg ccccggcctt cagggaacgc
    45421 ctcggcaatt ttgacgccac caaacaaccg tgcggcctat tgataaagag ctaacacatt
    45481 gtcaaaaaac atcactatgg ttttttagag tttctcgata tcaattgcct gaatagccct
    45541 tgcaatatca ggggaattat tcaacacccg aacatgctga aataattccg ttgcttcatc
    45601 gtattcttta cgcaaataac tcaaccactg tttaatccgc gcaacgtgat ataacccggt
    45661 atcgccctgc ttttccagac gggtatattt ttgcagcaaa gcaaccacct ccggccacgg
    45721 cattcgcggt tcgttatatt ttaccacccg gctcaggttg ggaatattga gcgccccgcg
    45781 accaatcatc actgcgtcgc agccgctgat cgccatgcat tgttgcgcgc tctgccagtc
    45841 ccagatttca ccgttggcaa tcaccggaat attcagccgc tggcgaatat cgccaatcgc
    45901 ctgccagtca atatgctccg cgcggtaacc ctgctctttc gtccgcccat gcaccaccag
    45961 ctccgtagcg ccagcctgtt gaaccgcatc ggcgatttca aatttcttct caccgctgtc
    46021 ccagcccaga cgcactttca cgctgacggg caaatgcgcc ggtacagctt cacgcatcgc
    46081 ttttgcaccc tggtagatga gttcaggatc tttgagtaac gtcgccccgc cgccgctacc
    46141 gttaaccgtt ttcgacgggc agccgcaatt gagatccacg ccccaggaac ctaactccac
    46201 cgcacgggcg gcgttctctg ccagccattg tgggaactga cctaacaact gcacgcgcac
    46261 cagcgtacca gatggtgtcc ggctggcgtt ttgtagctca gggcaaatgc gatgaaagac
    46321 ttttaccggc agcagttgat ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc
    46381 gttaacttcg gtcagcaatt cacgcaccag agagtcaagc actccctcca tcggtgccag
    46441 taacacactc atatcatcac ccgcaaaaaa atgaggcgct atgttagcgc ctatggtcag
    46501 cggattaaag gtctgcaaag agggattgct gcaaactgca atccctctct atgcttgatg
    46561 tatgcctgag ttcgctgcgc attgcgtaat gccggggaac gaaaaaataa gcgctgattt
    46621 cttaatgtga tcggtagcac gttttacgaa ttaattgtat gatgaatcca tctcatctgg
    46681 ggtgttgatt atgagtaaga cactgaacat tatctggcaa tatttacgcg ctttcgtcct
    46741 gatttatgcc tgcctgtatg caggcatttt cattgcttcc ctgctaccgg taaccattcc
    46801 gggcagcatc atcgggatgc tgatcctgtt tgtcctgctg gccttgcaaa ttcttccggc
    46861 aaaatgggtc aatccggggt gctacgtact gattcgctat atggcgctat tgtttgtgcc
    46921 gattggcgta ggcgtcatgc aatattttga tttgctccgc gcacagtttg gcccggtagt
    46981 ggtttcctgt gcagtcagta cgctggtggt ttttctggtg gtgagctgga gttcgcaact
    47041 ggtacacggt gaacgtaaag tcgtaggtca gaaaggatca gaagaatgat ggcgaatatc
    47101 tggtggtcat taccgctgac attgattgtc ttttttgccg cccgcaaact ggcggcacgg
    47161 tataagtttc ccttgcttaa cccgttgctg gtagcaatgg tggtgatcat tcctttttta
    47221 atgctgactg gcatctctta cgacagctac tttaaaggta gcgaagtgct aaacgatctg
    47281 ctgcaaccgg cggtcgtcgc gctggcctat cctttatatg agcagctaca ccagatccgc
    47341 gcgcgctgga aatcgatcat caccatctgt tttatcggca gcgtggttgc gatggtgacg
    47401 gggacttccg tggcattgtt gatgggcgct tcaccggaaa tcgccgcgtc aatcctgccg
    47461 aaatcagtca ccacgcctat tgcaatggcg gttggcggca gtattggcgg tattccggca
    47521 atcagcgcag tttgcgtgat tttcgtcggc atcctcggcg cggtatttgg ccatacattg
    47581 cttaatgcga tgcgtattcg taccaaagct gcgcgcggtc tggcaatggg gactgcctcg
    47641 cacgccctcg gtacggcgcg ctgcgccgag ctggattatc aggaaggtgc atttagttcg
    47701 ctagcgctgg tgttatgcgg gataattact tcgctgatcg caccgttcct tttcccgatt
    47761 attctggcag taatgggcta aaatttgcga tgcgtcgcgc atttttgatg tatgtttcac
    47821 gcgttgcata attaatgaga ttcagatcac atataaagcc acaacgggtt cgtaaactgt
    47881 tatcccatta catgattatg aggcaacgcc atgcatccac gttttcaaac cgcttttgcc
    47941 caacttgcgg ataacttgca atctgcactg gaacctattc tggcagacaa gtacttcccc
    48001 gctttgttga ccggggagca agtctcatcg ctgaagagcg caacggggct ggacgaagac
    48061 gcgctggcat tcgcactact tccgctggcg gcggcctgtg cgcgtacgcc attgtcgaat
    48121 tttaatgttg gcgcaattgc gcgcggtgtg agcggaacct ggtatttcgg tgccaatatg
    48181 gaatttattg gtgcgacaat gcagcaaacc gttcatgccg aacaaagcgc gatcagccac
    48241 gcctggttga gtggtgaaaa agcgcttgca gccatcaccg ttaactacac gccttgtggt
    48301 cactgccgtc agtttatgaa tgaactgaac agcggtctgg atctgcgtat tcatctgccg
    48361 ggccgcgagg cacacgcgct gcgtgactat ctgccagatg cctttgggcc gaaagatctg
    48421 gagattaaaa cgctgctgat ggacgaacag gatcacggct atgcgctgac gggtgatgcg
    48481 ctttctcagg cagcgattgc ggcggcaaac cgttcgcaca tgccttacag taagtcgcca
    48541 agcggtgtcg cgctggaatg taaagacggt cgtattttca gtggcagcta cgctgaaaac
    48601 gccgcattca acccgactct gccaccgttg cagggagcgt taattctgtt gaatctcaag
    48661 ggttatgatt acccggatat ccagcgcgcg gttctggcag aaaaagccga tgcgccgttg
    48721 attcagtggg atgccacctc cgcaacgctg aaagctctcg gctgtcacag tatcgaccga
    48781 gtgcttctcg cttaagcctg gtgccggatg cggcgtgaac gccttgtccg gcttgccagc
    48841 cctctcctgg tgtcgaaatt cccggcaaac agtttgccgt ttcttgcgca aaaccagcgg
    48901 gtaaagtagc ctgatggaaa ttttccttag atcgagtctc ctgcatgtta aagcgcgtgt
    48961 tcctcagcct gttagtcctg atcggcttgc tgctgttgac tgtgctcggc ctcgatcgct
    49021 ggatgagctg gaaaaccgcg ccttatatct acgacgaatt gcaggatctc ccctaccgcc
    49081 aggtcggtgt ggtgctcgga acagcaaaat attatcgtac tggcgtaatt aatcagtatt
    49141 atcgctaccg cattcaagga gcgattaatg cctataacag cggtaaggta aattatctat
    49201 tactgagcgg cgataacgca ttgcaaagtt ataatgagcc gatgaccatg cgcaaagatt
    49261 taatcgctgc tggtgtcgac ccatcagata ttgttctcga ttacgcaggc tttcgtacgc
    49321 tggattccat cgtgcgtaca cgcaaagttt tcgatactaa tgatttcatt attatcaccc
    49381 aacgtttcca ctgtgagcga gcattattta ttgcgctgca tatggggatt caggctcagt
    49441 gttatgccgt accgtcaccg aaagatatgc tgtcagtacg tattcgtgaa tttgccgccc
    49501 gtttcggtgc gctggctgac ctttatattt ttaaacgtga accgcgtttt ttagggccgc
    49561 tggtccctat tccggctatg caccaggtac cggaagatgc gcaggggtat cccgccgtca
    49621 cacccgaaca gttacttgaa ttacaaaaga aacaaggaaa gtagttatgg atgtacagca
    49681 gttttttgtc gttgccgttt ttttccttat cccgatattt tgtttccgcg aagcatggaa
    49741 aggctggcgc gcaggcgcga ttgataaacg ggttaaaaat gcaccggaac cggtgtatgt
    49801 ctggcgagca aaaaatcccg gactcttttt cgcttatatg gtggcatata tcggcttcgg
    49861 aattttatct atcggcatga ttgtttatct tattttctat cgttaatatt cccctctcca
    49921 gttaattatt gagaataatt attacttcac ctgataagct gcggatatca cattcctaac
    49981 cgcagctatt tgtgaatctt ttcacagttt aaattccccc gcacgcttag ccttaatatc
    50041 agtacattat tatttactaa acgctcgcct taattaccta tagcattaag gaagatcaca
    50101 tgccgcaaca aaattatctg gatgaactca ctccggcttt tacgtcttta ctggcgatta
    50161 aagaagcctc tcgctgttta ttatgtcacg acgctccctg tagtcaggct tgcccggcac
    50221 agaccgatcc ggggaaattt attcgctcaa tctactttcg taattttaaa ggcgctgccg
    50281 agacaattcg cgaaaataat gccctcggtg ccgtttgtgc cagagtgtgc ccgacggaga
    50341 aattatgcca aagcggttgt acccgtgccg gtgttgatgc tcccattgat atcggccgct
    50401 tacagcgttt tgttactgat tttgaacaac aaaccggaat ggaaatttat cagcccggta
    50461 ctaaaacgct cggcaaagtc gcgattattg gcgcaggtcc tgccggattg caggccagtg
    50521 tgacactgac aaaccagggt tatgacgtca cgatttatga gaaagaagcg caccccggtg
    50581 gctggttgcg taacggtatt ccgcaattcc ggttaccgca atcagtgctg gatgcagaga
    50641 tcgcccgtat tgaaaaaatg ggcgtgacca ttaagtgcaa caacgaagtc ggtaacacac
    50701 tcacccttga gcagctgaaa gcagaaaacc gcgcggtact ggtcaccgtg gggttatcaa
    50761 gcggttccgg gctaccgctg tttgagcata gtgacgttga gattgccgtc gacttcttgc
    50821 aacgtgcacg acaggcgcaa ggcgatatca gcattccaca aagcgcatta attatcggcg
    50881 gcggtgatgt cgcgatggac gtagccagca cgctgaaagt tctcggctgt caggcggtaa
    50941 cttgcgtagc gcgtgaagag ttagatgagt ttccggcaag cgaaaaagag tttaccagcg
    51001 cccgggagct gggcgtttcg atcatcgatg gattcacgcc agtagccgtg gaaggcaata
    51061 aagtcacgtt taagcatgta cggttatcgg gcgaactgac gatggcggca gataaaatta
    51121 ttctcgccgt cggtcagcat gccagactag atgcctttgc ggagttagag ccgcagcgta
    51181 acaccatcaa aacacaaaat taccagaccc gcgacccgca agtctttgct gctggcgata
    51241 ttgttgaggg tgacaaaacc gtggtctatg ccgtgaaaac cgggaaagaa gccgccgagg
    51301 cgattcatca ctatttagag ggagcttgct catgttaacg aaagatcttt cgattacttt
    51361 ttgcggcgtg aagtttccca acccgttctg cctctcttct tcgccggtag gcaactgcta
    51421 tgagatgtgt gccaaagcct acgacacagg ttggggcggt gtggtgttta aaacgatcgg
    51481 cttttttatc gccaacgaag tctcgccgcg ttttgatcat ctggtgaaag aagataccgg
    51541 ttttatcggc ttcaaaaata tggagcagat tgctgaacat ccgttggaag agaatctggc
    51601 cgccctgcgt cggctgaagg aagattaccc ggacaaagta ttgatcgctt cgatcatggg
    51661 ggaaaatgag cagcaatggg aggagctggc gcgcctggtg caagaagctg gcgcggatat
    51721 gatcgagtgt aacttctcct gtccgcaaat gacttctcat gcgatgggta gcgatgtcgg
    51781 gcaaagcccg gagctggtag aaaaatattg tcgggcagtg aaacggggtt ccacgctgcc
    51841 aatgctggcg aagatgacgc cgaatatcgg tgatatgtgc gaagtggcgc tggcggcgaa
    51901 gcgcggcggc gcagatggca ttgcggcgat taacaccgtt aaatccatca ccaatatcga
    51961 tcttaatcag aaaatcggta tgccgatcgt taacggaaaa tcgagtattt ccggatattc
    52021 cggtaaagcg gtaaaaccga tcgccctgcg cttcattcag caaatgcgca cccatccaga
    52081 actgcgcgat ttcccaatca gcggtatcgg cggcattgaa acctgggagg atgcggctga
    52141 gtttttattg ctcggcgcag caacgttaca ggtgaccacc ggcatcatgc agtacgggta
    52201 tcggatagtg gaagatatgg caagcgggtt gtcgcattat ctcgccgatc agggatttga
    52261 ttcgctgcag gagatggtag gtctggcgaa taacaatatt gtcccggcgg aagatttaga
    52321 ccgcagttat attgtctatc cccgtatcaa tcttgataaa tgtgttggct gtggacgctg
    52381 ttatatttcc tgttacgacg gcggtcacca ggcgatggaa tggagcgaga aaacccgcac
    52441 accgcattgt aataccgaga aatgtgtggg ttgtctgctt tgtggtcacg tctgcccggt
    52501 gggttgtatt gagctcgggg aagtgaagtt taagaaaggc gagaaagaac acccggtaac
    52561 gttgtaaaac cagagacgca tccggcattt ggttcctgtc gccggatgcg gcttgaacgc
    52621 cttatccggc ctacaaaacc atacaaatca gtatgttgag cttattgtag gccggataaa
    52681 acgcgccagc gttgcatccg gcatttggct cctgttgccg gatgcggcgt gaacgcctta
    52741 tccggcctac aaaaccacac agatcatgat gttatgtgcg tttatgaccg aatgcggacc
    52801 acattcacat catttcttac gcgcgtattt cagtgaatcc agcgctacgg cgaagataat
    52861 aatcgcccct ttgatgatgt actgccagta tgggtttacg ccgatatacg tcaggccata
    52921 gttgatgacg gtaaaaataa ttaccccggt caccacgcca atcaccgtcc ccacaccgcc
    52981 gctgaacgat acaccgccta ccacgcacgc cgcgatagca tccagctcat acataaagcc
    53041 gaggttgttg gtggcagagc cgatacgtcc ggcttctaac atcccgccaa aggcatagaa
    53101 cacgccagac aacgcgtaga tcatcagcag gttcaggccg acgttgacac cagatacttt
    53161 tgccgcttcc gggttaccgc caatggcaaa aatgttctta ccgaagcggg ttttgttcca
    53221 caacacccag acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag agagacggaa
    53281 actccccagc gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa aaccagaaat
    53341 tggcgacgcc ccgacaaagt cgtaatagag cgagttgatg ccatagacga tgatcatcgt
    53401 gccgagcgtg gtaatgaacg gcgtcacgtt gagataagcg ataatcagac cgttgatcaa
    53461 accgatcacc gcaccaatgg cacagacaat cagaataacc agcgcaatcg gcatcgtcgc
    53521 catttccggg aacactttgt tggcgttatc catggactgc aataatgtcg cagccaccac
    53581 tgccgccagc cctacctgac gaccagcaga aagatcggtc ccctgggtga caattaaccc
    53641 tgccacaccg agcgcgataa taatacgcac cgatgactgg gtgagaatat tacttaagtt
    53701 caacagactt aaaaatgttg ggtcctggaa aataataatc gccagcaaaa ctaaaagaac
    53761 gacgtaaata ccgccctctt tcaggtaagt aagaaaactt ttcttattta acgcactcat
    53821 gggaagcccc taatcttaaa ggtgcaaaga cgcaagacgc agaatttcgt tttgcgttgt
    53881 tgtttttgta tcgacaattc cggaaacgag accattgctc atgaccagaa tacggtctgt
    53941 tatccctaac aactcaggca tttcagagga gataataata atccccttgc ctttcttcgc
    54001 cagttcggca attaactgat aaatttcaaa cttcgccccg acatcaatac cgcgcgtcgg
    54061 ttcatcgagc attaatattt ctggttgcgt tagtagccag cgaccaataa tcaccttttg
    54121 ctgattacca ccggagagcg aaccaatttg cgtccgatga cccggcgttt ttacccgcat
    54181 cgaatcaatc acccactggg tatcgctttt catccgcgag ttatccagta aaccaacttt
    54241 atttttgtag ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca gataggcata
    54301 aattcccgtt gagcggcgct cctcagttac cagtgcaaat ccatggttta tggcttcgtt
    54361 ggcattatgg ttattgatct gtttgccgtg caacgttatg gtgccagccg atttctcgcg
    54421 aataccaaat aacgtctcaa caatatcggt acgtttcgcc cccaccagac cggcaatacc
    54481 gaggatctcc cctttatgca gatcaaacga gacatcgcga atcgacggct ggcgcagtga
    54541 cgtcaggtta cgtacctcga ggatgacttc gcccggtttg ttttctttgt cagggaaacg
    54601 ctggttaaga gaacgcccaa ccatcatggc gatgatcttg tccatcgtca gtcctgccag
    54661 cggttcggtg gcgatccact gaccgtcgcg caatacggta acttcatcac ataactggaa
    54721 gatttcttcc attttatgcg agatataaac aataccgcag ccgcgctctt ttaatttacg
    54781 aataatagtg aacagatgat tgacctcttt ttcggttaac gaagaagtcg gttcatccat
    54841 aatcacaatt ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct gcatttgcga
    54901 aacggataat gtgccgacgc gcgcacgcgg atcgatatca atatccagtt cgtcaaaaat
    54961 cgctttggtt tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg tgggatatcg
    55021 ccccagccac atgttatcca tcaccgaacg ttgtaatacc aggtttaact cctggtgtac
    55081 catcgaaata ccattttcca gggcttcttt tgcagaatgg aaatcgatct ctttaccctg
    55141 gaataaaatg gtgccggagt ctttttgata aataccaaac aggcatttta ataatgtcga
    55201 ttttcctgca ccgttttccc ccattaatgc atggatagaa tgtggccgga cttttaaatt
    55261 aacgttatca agtgccttaa caccaggaaa ggacttgttg ataccgctca tttccaacaa
    55321 gtattccccg gaggacggag tcgttgagct gaccatataa ttttaccttg ttggccatac
    55381 aataagggcg cagtaataga ctgcgcccaa tcagtcttat ttcttgctga attcagccag
    55441 gttgtcttta tctacgccaa cataaggtac gcggaccact ttgttgtcga ttttccagtt
    55501 ggtgccatca gccgcacctt taccatcggc caggtttttc gccagatcaa aggtcgcttt
    55561 cgcctggttg ttagcatcgt tcagtacggt gcccgccagt gcaccggatt tcaccagcgc
    55621 cagcgcttct ggcagcgcat cgacgccaaa caccggaatg ctggacttgt tgtgtgcttt
    55681 cagcgcttca accgcgccca ttgccatcgc atcgttgttg gcgataacca cttcgatttt
    55741 gttggcgttc gggccagaca gccaggcgtc catcttatct ttcgcctgag cggtgtccca
    55801 cattgcggta tctaactgta actgttcagt tttgatgcct ttatcgttca attctttaat
    55861 cacgtaagtg gtacgtgctt ctgcatccgg atggcccggt tcacctttca gcagtacgaa
    55921 ctgaatctga ccgtctttgt tcagatccca accctgattc gccgcccagt gtttagcaat
    55981 caaatcgcct tgaataatgc cggactcttt ggagtcagtg ccaacgtagt aggctttgtc
    56041 gtagctatcc agcgccttac gagacggttc tttgttgaag aaaaccaccg gcacgttttg
    56101 cccacgcgct ttctcaatca ccgtacccgc agctgccggg tcaaccaggt tgattgccag
    56161 tgccttcacc cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg actggtcatt
    56221 ctgagaatca ttcatcagca gctgaacatc tggcgcggct ttcgcatctt gctcaatagc
    56281 cttgcgcact acagacataa agttatcgtc gtacttatag attgttacac caatgcgagt
    56341 atcagcagcg tgtgcagcgg caccgaataa catgctggcc atcacagcag acagggttaa
    56401 caccttctta ttcatggtat ctccggtttt tcttatgcag ggtagtgctt gagataaatg
    56461 ctcggcgggg cagtagagtt aatgaagtgt tactgaacgc cgaagctcac tttttaaaat
    56521 tcgttcttcc atgcccggta acgctccaga aaacggcttt aattgttgtt atgacgctgt
    56581 tacctcggca aaagtgatta ttcactgtta catacgggtt acaacgttaa aacggtgcaa
    56641 tcatagctat cacattgtta agatactgtg aaatcactca cagattgaaa gcggttacat
    56701 cgcctgattt gttgagttag tgatcgccgc cgcattctgg cgtgttgcga cagaatggcg
    56761 acgcactaac gtcggcataa aacagtggct ggcacgagga tcaatattgc ctgctgcccc
    56821 ctgcaaggcc agttcggtgg ctaatttcgc cattgaagca atgggataac gcacggtcgt
    56881 taattgcggg tcggtgtaac gggcgatggg aatatcatcg aaaccgatga ttgagagatg
    56941 taacggaatc gcaatgccat tatcttttaa tgctgtcagt gcgccagcgg ccatattgtc
    57001 gttataagca aatacagcgg taagttgtag attgcgcccc agcagttcaa ccatcgccgc
    57061 ctcaccgccc ggcatgtccg gcgtaccagc gccaatccag ctttccggcg gaataatatc
    57121 ctgctctttc aacgcactca tccagcctgc tttacgcatg gcgtcatctt caatgccgtg
    57181 gctggaagaa agataaccaa tacgttgatg accgttattc agcaacatgc gcgtcgccat
    57241 tcgggcaccg ctgagattat ccaggcaaac gcaacgatgg gcgtaccccg gcacaacgcg
    57301 gttgattaac accataccgg gaatgttatc cataaattgc gccagttcat cgtcactcaa
    57361 tgcttttgag tgaacaatca acgcattaca acgctggcga attaacacct caatggcgtg
    57421 acgctctttt tccgcttcat gatagctatt gccgattagc acgtatttct gatgctgctg
    57481 agcgaccaga tccaccgctt ttaccagcgc gccgaaaaac gcatcagaaa cgtccatcac
    57541 caccacgcca atggtgtcgc taacctgagt tgccagcgcc tgggcattgg cgtttggccg
    57601 ataatccagc tcactcacgg ctttcattac tgcttcacgc gtgtcggcac tgaccagcgt
    57661 gctgttattg agcacccggg aaaccgttgc cacagagacg ccagcctgac gcgctacatc
    57721 acgaatggtg atcatattca ctacctgtta tgagattgca gtaaatgact gcttgctggc
    57781 ggctattttg tcagcactga atacaggact tcgtgaatcg agtcacagca atggaaacgg
    57841 ttacagccgt tttgttaacg aatgtgatcc agatcgttat cttgatgttt tagatattgc
    57901 cggtccggca gcacgcaaag ttaactgacg ccagagccat tccaccggcc cctggcggaa
    57961 gtaacgcagc cagataacag agaagaggat attcgccagc cataccggaa taacaaacgc
    58021 cagcagctcc aggcggtcaa aatgcataaa caaaccgagg tggtaaaaaa gcgtggtaca
    58081 aatcagcgtt tgcaatagat agttggtcag cgccatccgt ccgacgcagg cgatcgcaag
    58141 caccagctta aagcggctca attgcggcca gaagccataa aacagcgacg catagccaat
    58201 cgcctgaaac ggcgcactca gttcccgcgg catttgaagt aagaaggcac accagcgata
    58261 cgcccagtcc agctgccatt gcagggcgat ggcaggaagg ttaatggtca ccccaatcgc
    58321 caccagcaca aaaccagtac gacgatagtg acgtaagctg aactgccctt tcagccagcc
    58381 gctgcgcatc agcgccgcac caatgagcat catccctgcc agttgccagc catactgtgc
    58441 gcccagtgcc agtaaactgt tgccaacacc atcggcacga ttactgatcg cttcaacgcc
    58501 gccgtgaagc ttccagtatt tttcatacaa aatagccgat gcatccggcg tccaggcgcg
    58561 gctggtctgg ctgtcggaaa tcagccccaa taacagcaaa acgccaagcc ccaccagata
    58621 aagcatgacg cctgtattaa acaggctttt taccgatggc gcatcgcgca ccagccgcca
    58681 gcagattaag cccaccagcc cgtaagccag caaaatatcg ccgtcccaga acaataaacc
    58741 gtgaataaag cccaacaaga ctaacagcgt taaccgcgac tggatccagc gtctgccacg
    58801 gggcagcaac atttgcaggc ccgcaccaaa cagtagcgca aaaagcgtga ggaatttcac
    58861 ctggccgatg agatcaagaa atgcccaagt ccatgcatcc cgcggcgtaa tagcaccgta
    58921 ccaggcggga ttgagataag ccgcctttgg tagcccaaag gcgctgatgt ttaatagcag
    58981 gatccccaga atggcgacgc cgcgaacaaa atcgagcgtg acgttgcgct ccatggttcc
    59041 tgccttttaa tcagttgtga tgacgcacag cgcgcagaaa ctcgtggcgc gtattctgac
    59101 tggatttgaa caatccacca agagaggtcg ttgtcgtggc actggttgca tcgcggatgc
    59161 cacgcgcctt cacgcagtaa tgcaccgcgt cgatcgagac agccacgtta ttggtgccca
    59221 gcagcgtttg tagcgcaata agaatttgct gcgtcagacg ttcctgcacc tgcggacgct
    59281 gggcaaagaa ctgcacaatg cggttaattt ttgacagacc gatcaccgaa tctttcggga
    59341 tataggccac cgtcgctttg ccatcgatgg taacaaaatg gtgttcacag gtgctggtca
    59401 gagtgatatc gcgcacggtg accatttcat cgaccttcat tttgttttca atgagggtga
    59461 ttttcgggaa attggcgtaa tccagaccgg agaaaatttc atcgacatac attttagcga
    59521 tgcgatgcgg cgtttccatc aaactgtcat cagccaggtc gagattcagc agctgcatga
    59581 tttcggtcat atgaccagca ataaggcttt tgcgcgtttc gttatccatt tcatgcacgg
    59641 gcgggcgcag cggtgtttcc agtcctcgcg caactaacgc ttcatgaacc agggccgctt
    59701 ctttactgag tgatggcatt tatgatttct cctgcaggtg tgacgcctcc gccctgcgtg
    59761 ggggcaaagt tattaagctg atttacagcc tgattattgt gcgtgaggcg gcgcacataa
    59821 tccagtattc acagcgataa ttattgtaat tgccgctgcc tttcatcagc agatgttaaa
    59881 acatcgttat gcaaatacgg aagtgaaagt tactcacagc acattgaata aacggtatga
    59941 tgaagaaatt gcaaacaaca caacaaggag ccacgcatgg aaatgctcga agagcaccgc
    60001 tgttttgaag gctggcagca acgctggcga cacgactcca gtaccttaaa ctgcccgatg
    60061 acgttcagta tctttctccc cccacctcgt gatcacactc cgccaccagt gctgtactgg
    60121 ctttccggat taacctgcaa tgacgagaac ttcaccacca aggcgggtgc ccagcgggta
    60181 gcggcggaac tggggattgt actggtgatg ccagacacca gcccgcgcgg cgaaaaggtt
    60241 gccaacgacg atggctacga tttaggccag ggcgcaggct tttatcttaa tgccacgcaa
    60301 ccgccgtggg cgacgcatta ccggatgtat gattatctgc gcgatgaatt accggcgctg
    60361 gttcagtcgc aatttaatgt cagcgaccgc tgcgccatta gcggtcactc aatgggtggt
    60421 cacggtgcgc tgattatggc gctgaaaaat ccgggtaaat acaccagcgt ttcggccttt
    60481 gcgccaattg tgaatccgtg cagcgtcccg tggggaatca aagcgtttag cagctattta
    60541 ggtgaggaca aaaatgcatg gctggaatgg gacagttgcg cactgatgta tgccagtaac
    60601 gcgcaggatg cgatcccgac gcttatcgat cagggcgata atgatcagtt tcttgccgac
    60661 cagttgcaac ctgcggtact ggcagaagcc gcgcgccaga aagcgtggcc gatgacgctg
    60721 cgtattcagc cgggatatga tcacagttac tacttcatcg cctcttttat agaggatcac
    60781 ctgcgcttcc atgcgcagta tttactgaag tgaaagtccg cccggttcgc cgggcatctt
    60841 ctcatcagaa gcgataatcc actgccataa agtaacgacg tccgtcttcg ttatagctgt
    60901 agtcgtcacg actgagatcc ttgtcgccaa ggttcagcac gcctgcgcgc agtttgacgt
    60961 ctttagtcac ctgccaggcc gcgccggtat tccagatggt ataaccgccc ggtgttttag
    61021 ccgtcgcgct gtcggcgcgt ttctgcccgg tatagtgccc agaaacatag aatgaccagt
    61081 cttccagcgc cagcggtttc cagtccagcg taccgttagc agtatggaac ggcagatcgg
    61141 atagcggttt gttttcgccg ttgctgacat cacgaccatc gttgtaggtg tagttgatcg
    61201 acagtttcca ttcatcgttg aacggaattt tcagttcggt ttccacgccc tgaatacgag
    61261 ctttgttaac gttgtagtag ctaaataccg gtatgcgccg tccgttagcg cccgtctcaa
    61321 aaccaacaaa gttttggtag cccggtgcag cgttgacgtc agacgtacgg ctgatgctga
    61381 tacgatcttt cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc
    61441 cttcttcacc catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat
    61501 ccgggctacc cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa
    61561 gttgcaacag agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt
    61621 cggtggcgtt ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcgtgat
    61681 cgtccatacg cacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcca
    61741 caaacagcgc gtactggctg gcagacgttt tggagctggt tcccccggtc aggttcaccg
    61801 catcgctaag tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg
    61861 tcagcggcaa cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt
    61921 tgccagggtt tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat
    61981 cccaacgccc attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg
    62041 agtcggaatc acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat
    62101 tcggtgtcca ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg
    62161 tttctccggt atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc
    62221 tgccgtaagc tttcattccc agcacaatca attaatggtc cactggtaaa gaactgaccg
    62281 ttataggtgt caccgcgatc gcgatgttcc tgaatggtgg tatcgacggt aacggtaccc
    62341 gaccatttct gaccgatttt tttggtgatg atattcacta caccgccgag cgcatcggaa
    62401 ccgtacagcg acgacatcgg gccacggacc acttcaatac gttcgatgga atcgaccggg
    62461 atccagttca gatcgaaatc attgtggcgg aagacggcat tgcgggagtt cacgcgttta
    62521 ccgtcgacga gaatcagggt atagctgctg tccagaccac gaatactaac gcccttacgg
    62581 ttatcccctt cgttcgtcag ttgtacgcca ggcacttctt tgaggacatc cttcagattc
    62641 tgtaccggtt ttcgctgcag gtcttcctgg gtaatgacgc tgatactggc aggtgcatct
    62701 ttaagatttt gttccacgga agatgcagtg acaaccatcg tttcgccatc atcatcgacc
    62761 gctaacacag gccatgcaca agaaatagcg gacaaacaca gcccgacccg tacgaaaggg
    62821 ttcaacctaa acattccata tctccatgag gtaactacga aaataaaatg ggttatcgct
    62881 cacatcttct tcacgtcccc ttgcgtacgg cagcatcgcg gtggacttat tttttatgca
    62941 ggtgatcatc ccagaaagcc ggaacagcct ctgatttgtc agcctttttt gattgcggcg
    63001 taacgataat gcaaacgata acaattatca atccaaatgt taaattttat atccgcatga
    63061 tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag caaggaaggg
    63121 aggaaactta tttcttatcg ttctgcggga acttcatttc gctgtagcgt acgaagtgag
    63181 ttcctttaat cagcttgtag ccgaaccaaa taatcaggaa cagcgggata ccaatatacg
    63241 ttgccgctac gccgccccag tcaatagtat ctttcaggaa cgcttcgtag ttctggccca
    63301 aagtgataat cagacacaga atgaatgcga agatcggccc cagtgggaag aaacctgaac
    63361 ggtacggcag atcgttaatg tcgtgtccct gcaatacgta accgcgacgg aagcgatagt
    63421 ggctaatggc aatccccagc caggcgataa aacccgtcat cccggaggtg ttcagcagcc
    63481 acaggtatac cgtctggttg ccaaacatgg aggtcaggaa gcacagaccg gcaatcaccg
    63541 tcgtcgcata cagcgcatta cgcggcacgc caccacgcga cagtttagcg aaaatgcgcg
    63601 gcgctttacc gtcacacgcc agggtgtaca gcatacgagt agacgcatac ataccggagt
    63661 tacccgctga cagcaccgcc gtcagaataa ctgcgttcat caccgccgcc gcagagagca
    63721 gacccgcgtg ctggaacacc agggtgaacg gactaacgct gatgtcttta acatcgttac
    63781 gcagcaggct cggatcggtg tacggaataa tcaggctgat aatcaggatc gcgaacacat
    63841 agaacaacag gattcgccag aacacctgac gtaccgcgcg tggaatgttt ttcgccggat
    63901 cttcggactc gcctgcagca ataccgatca gctcggttcc ctggaaagag aagccgacaa
    63961 tcatagctac gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg ccgattgtcc
    64021 agttgctcca gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc agcacgccaa
    64081 cgatgataaa gacaataact gtcgtgactt tgatcagtga gaaccagtat tccgcttcac
    64141 caaagccacg aactgagatg tagttcagca ggaagataac gccgaggaac aacgcactcc
    64201 agatccagcc cggtgtatcc gggaaccacc agctcatgac cagctgagct gcaaccaggt
    64261 caacggcgat agtcaccgcc cagttgtacc agtagttcca gcccagcgcg aagccaaagc
    64321 cttcttcaac atagttctga ccgtaagtgg caaacgaacc ggaaaccggc atatatgcag
    64381 ccagttcacc gagactggtc atcaggaagt aaaccatcag gccaatcagc atatacgaga
    64441 gcaatgcccc gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca aaaagacctg
    64501 taccgatgga accgccaatg gcaatcatcg tcaggtgacg cgcctttaat tcacggcgta
    64561 agcccggcgc ttctgtggtt ttagtttcgg aaaccataca aaaatgctat ccatcttaaa
    64621 aatgaggcgc gattgtagca gacgattggc aatccttccg gcagaaatac acggttataa
    64681 gacaccttca tgatcgccca gggattataa gtaaagcagc caatctcttt tctggcgaat
    64741 gcatgctgaa ggcgctgcgt cacgaaatcg acacagcacc agcatgttct tgtacagcaa
    64801 cttaacgcgg cacatttgcg ggatcgcaat agtccagaaa gcgccgtagc gcgttggaaa
    64861 ggtgtttttg ccgatgatgt atacgccaca acgtacgcat caggcgcggc agagggaccg
    64921 caacttcact taatgtgcct gcctgcaatt gatcttcaat cacacgtcgc gacaggcaac
    64981 taattcccaa cccatgacgc accgcatgtt tgattgcctc ggagttacct aattccatcg
    65041 ccatctcaaa cttcggtaaa tgtgacagca acagataatc gacaatctcc cgcgtgccgg
    65101 aaccgcgttc acgcaggatc cacggcgcag cggccagctg ttctaaggtg accggaccac
    65161 gggccaacgg cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc cacggttcag
    65221 aaatgatttc agtgctgtgg cacggtcctt caataaagcc aatatcaacg cggaaatcca
    65281 gcaccgcttg catcacgtcc tggctattcc caacgctaag ttcaatcggc aactgcggat
    65341 aatcatggcg ataacgggcg ataactgcag gcagaatgta gttaccgatg gtactactgg
    65401 catagatacg aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca accgcctgtt
    65461 caagcaatgc caacgcacgc ggatagagca gccgcccgtg ttcattaaca accagtcttt
    65521 tccccacgcg atcaaacagt tgcacgccaa gctgcccttc caggtcggtc aaggctgcgc
    65581 tcactgctga ttgcgacaac gccagcatca ccgacgcctg ggtggttgat ccacttttca
    65641 atacttctgc aaaaacttcc aactgccgga gggtgatgtg catagtcgct taccacttat
    65701 aaagattaat tataaatata taatcaattt tatttttaaa ccagttagtc gtaaccttat
    65761 gcccggttaa aggagagggt tatgacgaat atcaccttac agaaacaaca tcgtacactg
    65821 tggcatttta ttccggggtt agccctgagt gcagttatca ccggggtcgc cctgtggggt
    65881 ggttccattc ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc aatcttgttg
    65941 gggatggttt taggcaacac catctatccg cacatctgga aaagctgtga cggtggtgtg
    66001 ctgtttgcca agcaatattt attacgtctg ggtatcattc tttatggctt ccgtctgacg
    66061 ttctcgcaaa ttgccgatgt cggtatcagt gggatcatca ttgacgtgtt gacgctgtcc
    66121 agtaccttcc tgctggcttg cttcctgggg cagaaagtgt ttggtctgga taagcacacc
    66181 agctggttga tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact ggcgactgag
    66241 ccggtagtga aagcggaagc cagtaaagta accgtggctg ttgcaaccgt tgttatcttc
    66301 gggaccgtcg cgattttcct ctacccggcg atatatccgc tgatgtctca atggtttagt
    66361 ccggaaactt tcggtatcta cattggttct actgtgcacg aagtggcgca ggtggtggcg
    66421 gcaggtcatg ccatcagccc ggatgcggaa aacgcagcag ttatttccaa aatgctgcgc
    66481 gtgatgatgc tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca gctgtctggg
    66541 gcgaacagcg gcgagaaaag caaaatcact attccgtggt ttgccatctt gttcatcgta
    66601 gttgccatct ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat gctggtaacg
    66661 ctggatacct tcctgctggc aatggcgatg gcggcgctgg gtctgaccac tcacgtcagc
    66721 gcgctgaaaa aagctggggc gaaacctctg ctgatggcac tggtactgtt tgcctggctg
    66781 attgttggtg gtggtgcgat taactatgtg attcaaagcg tcatcgcata aaccactaca
    66841 tcttgctcct gttaacccgc tatcattacc gttttcctcc agcgggttta acaggagtcc
    66901 tcgcatgaaa tacattggag cgcacgttag tgctgctggc ggtctggcaa atgccgcaat
    66961 tcgcgccgcc gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc aacgtcagtg
    67021 gcgtgccgca ccgctcacga cgcaaaccat cgatgaattc aaagccgcct gtgaaaaata
    67081 tcactacaca tcggcgcaaa ttcttcccca cgacagttat ctgattaacc tcggacatcc
    67141 ggtcactgaa gctctggaaa aatcgcgcga tgcctttata gatgaaatgc agcgttgcga
    67201 acagctgggg ctttctttgc tcaacttcca ccctggcagc catctgatgc agatttcaga
    67261 agaggattgc cttgcgcgta ttgccgaatc catcaacatt gcgctggata aaactcaagg
    67321 tgtgacagcg gtgatagaaa acaccgccgg tcagggcagt aacttagggt ttaaattcga
    67381 acatctcgcg gcgattatcg acggcgtgga agataaatcc cgcgtcggcg tctgcattga
    67441 tacctgccat gctttcgctg ccgggtatga tttgcgtact ccagccgaat gcgagaaaac
    67501 attcgcggat tttgcccgta ctgtcggctt taagtatctg cgcgggatgc accttaacga
    67561 tgcgaaaagc acctttggca gccgcgttga ccgccatcat agcctcggtg aaggcaatat
    67621 cggtcatgat gcgttccgct ggatcatgca ggacgaccgt ttcgacggca ttccgctgat
    67681 cctcgaaacc atcaacccgg atatctgggc agaagagatc gcctggctga aagcgcaaca
    67741 aactgaaaaa gcggtagcct gaagatgaat aaccgggaaa aggagatcct tgcaatttta
    67801 cggcgtaacc cgctgattca gcagaacgaa attgcggaca tgctgcaaat cagccgttcg
    67861 cgcgttgcgg cgcatattat ggatttaatg cgcaaaggcc ggattaaagg caaaggttac
    67921 attctcaccg agcaggaata ctgcgtagtg gtggggacaa tcaatatgga tattcgcggg
    67981 atggcggata tccgttaccc gcaatcggct tctcatcccg gtacaattca ttgctcagcg
    68041 ggcggcgtgg gacgcaacat cgcccacaat ctggcgctgt taggccgtga cgtccatttg
    68101 ctttcagtga ttggcgatga cttttatggc gaaatgctcc tggaagaaac gcgccgtgcc
    68161 ggcgtgaatg tctccggctg cgttcgtttg catggtcaaa gcacatcgac gtatctggca
    68221 attgccaatc gagacgatca aaccgtgctg gcgattaacg atacccatct gctggaacag
    68281 ttgacaccgc aactactgaa cgggtcgcgc gatttacttc gtcatgcggg cgtggtactg
    68341 gctgattgca acctgacagc cgaggcgctg gaatgggtct ttaccctcgc tgatgaaatc
    68401 ccggtgtttg tcgataccgt ttcagaattc aaagcgggca aaatcaaaca ctggctggcg
    68461 catattcaca ccctgaaacc cactttaccg gagctggaaa ttttatgggg acaggcgatc
    68521 accagcgatg ctgaccgtaa taccgcagtg aatgcattgc atcagcaagg tgttcagcaa
    68581 ctgtttgttt atttgcccga tgagtcagtt tattgcagcg aaaaggatgg agaacaattt
    68641 ttgctgaccg cgccagcgca tacgacagta gacagttttg gtgctgacga tggttttatg
    68701 gcgggcctgg tatatagctt tcttgaagga tacagtttcc gcgacagcgc ccgttttgcg
    68761 gtagcctgcg cggcaatttc gcgcgccagc ggcagcttaa acaaccctac cctgtctgcc
    68821 gataacgcgc tttcattagt gccaatggtg taacaatgtt gccggatgcg acgcttaacg
    68881 cgtcttatcc ggcctacaaa tcaggcaatt tcagcaagta aatgtaggcc tgataagctt
    68941 gcgcatcagg caatgacact caagctaaac caataaagaa cccggcaatg gtcgcactca
    69001 tcaagttgga aagcgtcgcc gccgccagcg cccgtaaacc aagctgggcg atttccggcg
    69061 cacggtgtgg cgcaaccgca gaaaacgccc ccaccaccac cccgatagaa ccaaagttag
    69121 cgaaaccgca caacgcgaag gaaataatcg ccacagtttt agcatcgaga gtgccagccg
    69181 tttgcagata gggtgagaaa ttgagataag cgacaaattc atttattgcc agtttctgtc
    69241 caatcaaact cccggcaaga ttcgcatcac tccagtccac acccatcacc cacgccagtg
    69301 gagccagcag gtaacctaaa atggactcca gcgaggcatg ttcaaaacca aaccagccac
    69361 caacgccgcc gataataccg ttaatcaacg caattattgc aacaaatgcc atcaccactg
    69421 ttgccacacc tgcggcgatt ttcagcccgg tcattgcccc tgtcgcagcg gcttcaataa
    69481 tgctttttgg cggtgtttcg gtgaaagaga ggttattaaa ggaaacctgc gaagattccg
    69541 ttgccgggct taacaggcgg gcaaacaaga tcccgccagg gatcgccatt aatgatgccg
    69601 ccagcagata ttccacaggc acgcccagtg cggcgtaacc aatcattgtc gaaccagcaa
    69661 tcgaggccat gccactacaa atcgctgtaa ataattcatt gcgattcaga cgatcgataa
    69721 agggtttgac gattgccgga atttcgtttt gcccgaggaa aatggtggtg accgcgacga
    69781 atgactcgat cttgctgata tttaatgctt tctggaagat accgccgaga attcgaatta
    69841 aaatccccat cacaccgatg tagtagagaa tactcaccag cgcggtgacg aagataattg
    69901 ccggtaacac cctgaaacca aagataaatc ctgcaccatc aaataaggta tccattttcg
    69961 gtccgaccag agaaccgaag ataaatgcgc tacccgcgtc gctgtacgcc atcactttat
    70021 gcacgccaaa agcgactttt tcagcgaccc aacgccctgg cggtaaccaa agcataatgc
    70081 cgccaatcac gacctgtaac actaacgccg cgccaacggt acgcaggctg atcttcttct
    70141 tgtttactga cagtaaaaac gcaatcgtca gcaataccac cattcccaga acacttctca
    70201 tgacatccat agtgattatc tcttcatgtc atattgggcg acggctttct gcccatcaga
    70261 aatagccagg cctgcatctt gcgataccgg cccggcttgt tatcaacact taatgggttt
    70321 tgatgtagcc gcgcacgcac tcttcgacta atccccagaa ccagtctgta tcaatagtga
    70381 tgccgacttt ggtattggcg ggcttgccaa gaacgcccag ctcgtcgcag acggtacgcc
    70441 catagcaagg gccactgttg acgtccactt cgacgtacat ctcctgggtt ttaatgccat
    70501 cagggttaat cagataaccg atgcaggtgg cgtcgtgcac cgggccgcca gcaaggccgt
    70561 agttttcgaa ctgcgttttg agagtgaagt tcatgatgtc gctgaacagc tctccggcgg
    70621 ggccgcctgc cctttccatc cgagcaatca cgtccggggt gcaaacggtc tggttggtga
    70681 gatcgaggcc catcatcact aatggaacgc cggaggtgaa cactacgcgt gcggcttccg
    70741 ggtcggcaaa gatgttgaac tcggcagatg gcgtgaagtt gcctgtaccg taagcgccgc
    70801 ccattagcac aatttcacgg attttgggca ggatcgcggg ttgcatacgc attgccaccg
    70861 cgatatttga aagcggacca accggcacca gagtgatatc gccatcgctt gccatcaggg
    70921 tatcaatgat atatttcacc gcatgagtgc tttctgcctg gcgggtcagc ggctcgaata
    70981 ccgggccatc cagtccggtt tcaccgtgaa tattatcggc aacgatttgt tgacgcataa
    71041 tgggctgcgg catccccgca taaaccggaa cattaatctc cagtttctgg caaacattca
    71101 ggccattaat taatgtttta tcaagcgtct gattacccgc tacaatagtg atgcctaata
    71161 aatctattgc cggatgtttc gccgccatca ttatagcaat agcatcatca tgacccggat
    71221 cacaatccag aataattttt ctcttttcca ttgtttattt cctctgtttc cagttgcgtt
    71281 attttttcta cagcaaagaa aattcgaccg ggccgatgat tgaatcttaa caacagcgta
    71341 cgtatgctaa atatgagaaa tctcatagcg gataaacatc gtgaaagaaa tccacaataa
    71401 tgatcttaag cagcaattga tgagtgaatc cgcgtttaag gattgctttt taacggatgt
    71461 ttcagccgat acgcggctgt ttcatttttt agcgcgtgac tacattgtgc aggaagggca
    71521 acagccgtcc tggctgtttt acctgacgcg aggccgcgcc aggctttacg ccacgctagc
    71581 taatggtcgc gtgtcgctga tcgatttctt tgccgccccc tgttttattg gcgagattga
    71641 gttaatcgat aaagaccatg aaccgcgtgc ggtgcaggct attgaagagt gttggtgcct
    71701 tgcgctccct atgaaacatt accgtccgct gttattaaac gacacgctat ttttacgaaa
    71761 actctgcgtc accttaagtc ataaaaatta tcgtaatatt gtttctttaa ctcagaatca
    71821 atcatttccg ttagtgaatc gcctggcagc atttatatta ctctcgcagg aaggtgatct
    71881 ttatcacgaa aagcatacgc aagcggcaga gtatttaggc gtttcttatc gacatctttt
    71941 atatgttctc gcgcagttca ttcacgacgg tttattaatt aaaagcaaga aagggtatct
    72001 cattaaaaac agaaagcagt tgtcaggact ggcgctggag atggacccgg agaataaatt
    72061 ctccgggatg atgcagtaaa aattattttc aatagcgcga ttgccggatg caacgcttaa
    72121 tacgttttat ccggtctaca aatcgagcat tacgccagac caataaagaa cccggcaata
    72181 gtcgcactca tcaggttgga aagcgttgct gctgccagcg cccgtaaacc aagctggggg
    72241 atttccggcg cgcgttttgg cgaaatagcc gaaaatgcgc caacgacaac accgatagaa
    72301 ccaaagttag caaaaccaca aagcgcaaag gagataatcg caatggtttt cacttccagc
    72361 gtgccgcccg tttgcaggta tggggagaaa ctcaggtaag cgacgaattc gttaatcgcc
    72421 agtttctgcc caatcaggct acccgcaaga ttggcatcac tccagtccac acccatgatc
    72481 cacgccagcg gtgccagcac atagccaaaa atactttcca gagaggcatt ggcgaaacca
    72541 aaccagccgc caattccgcc gataatgccg ttgatcagcg caataattgc gacaaacgcc
    72601 attaccaccg tcgccacacc agcggcgatt tttagcccgg tcatcgcacc gctcgccgcc
    72661 gcttcgataa agctttttgg cggcgtttcg ctgaacgaca gattttcaaa tgtgacctgc
    72721 gaaggctcgg ttgccgggct aagaatacgt gcaaacaaaa tcccgccagg gatcgccatc
    72781 agcgatgccg ctaacaggta gtcaattggt acgcccattc cggcataacc aatcatcatc
    72841 gaaccagcaa tggacgccat cccgctacaa attgcggtaa acaactcgtt gcgattcatg
    72901 cgatcgataa acggtttaac gatcgccggg atctcatttt gcccgaggaa aatagtagta
    72961 accgcaacaa aagattcgat tttgctgatg ttgagggctt tctggaaaat gctgccaagg
    73021 atgcgaatca gcagccccat cacgccaatg tagtacagca gactgatgag cgcagtaacg
    73081 aaaataatcg ccggaagtac gcgaaaggcg aagataaaac ccgcaccgtc aaacaggaca
    73141 tccattttcg gcccaaccag cgaaccaaaa atgaaggcgc taccggcatc actgtaagac
    73201 atcactttat gaacgcctaa tgccgcctgt tctactgccc attttcccgg tgggaagtag
    73261 agcatgatgc caccaatagc gatttgcagc agcagtgcgg ctccaaccgt gcgcaaactg
    73321 atgctctttt tattcactga caacagaaat gctattgcca gtaacaccac catccccaca
    73381 acacttctca ttatatccat aatgattttc ccttcatgcc ggtaaacccg gcgtcagcgc
    73441 caggttttgg tatgcttgat gagtacgggc gacggctttc tgcccgtcag aaattaaccc
    73501 gcgagacgct gatattcttt ggcaatttcg ctcgccagaa tggcgttgtt gaacaccagc
    73561 tggatgttgg atttcaggct gtcaccgccg gtcagttcag caacgcgcgc cagcaggaat
    73621 ggcgtacttt ctttaccaat gactccctga gcttcagcta ccgcctgatc gatcgccgca
    73681 ttgatagtgt gttccggcat agcaaactgt tccgggatcg ggttcgctac cacgaggcca
    73741 ccgttcagac cgctttgcca tttcaccgcc attgcacggg caatttcgct ggcgctgtcg
    73801 agacgaatgc tgacgtcaaa cgggctggtg cggcagaaaa acgcaggcag cgctttagtc
    73861 tgatagccaa ttaacggcac accgaaggtt tctaaatact cagtggttaa tcctaaatcg
    73921 agaatagatt tcgccccggc acaaacaacg gtgacattag tatttgccag ttcttgcaaa
    73981 tcggcagaaa tatcgaaggt atgttccgcc ccgcgatgca caccaccaat tcccccggtg
    74041 gcaaatactt taattccggc aagcgccgca ataatcatcg ttgaagccac agtggttgcg
    74101 ccattttttc cggcggcaac aacaaaaggt aaatcgcgac gactaacttt ggtcacgtta
    74161 tgcccttcac gacccagtaa ttcaatttct tctttgctta accccacttt catcacgccg
    74221 ccaataatgg cgatcgtggc aggtacagcg ccctgtttac gaatagtttc ttcaacttca
    74281 attgcggtct gggcattttg tgggaacggc atcccgtgag aaataatggt cgattccagc
    74341 gccacaaccg gttttttgtt ttttaaagcg tcctgcactt ccggggaaat ttgtaataat
    74401 tcaggggaaa tttttaattc agacattctg cgttctccac taacgatata acgttggcaa
    74461 tcgataaatc ggggttattg gtgtattcac aggagagcgc cattgacgaa catccctgtg
    74521 cgaaacgaac agattcggca aacggcattc cgtctaccca acacgaagca agtcccgcca
    74581 tcatggcatc gcccgctccg gtaacattaa taacattggt tttgatcggc gcagaccagc
    74641 cactttcacc gctgatatcg ctgtaataaa cgccgtcgcc gcccatgctc aataccagtc
    74701 ggttcaggcc atgttgatgg aaccaggcag caacttttgc cacatcttca cgccctgaca
    74761 gcgcaatccc actcagggtt tccgcttcaa ggcggtttgg cttgagagtg tggatctgat
    74821 ttagacggtc gcgcactttg acacatttcc atgccgaaac cggatcgaca aatacgggta
    74881 cgttggcggc attatccaga atccatgcca gtgcctcttc actgatatta cagtccgcga
    74941 caatgacctt tgccctctga ataaattcac cgtgctgtgc gagatattca gctgtaatag
    75001 cgttgctaat attcatgtca tttatagcaa ccagcatttc accggtatta tcgagtaatg
    75061 ataaataact cgacgtattt tctcccggca caatcaggca tttatcgaca taaacgccag
    75121 attgattggt ttgcgttagc agcgattgac cataaaaatc actgcctacg gcgctcagta
    75181 gccaggcttt gttacccagc aacgccaggt tttgtgcaat attgcgccct actccaccag
    75241 gcgtaaattt tattttacct ggatttgaat ccgcataatt taatgattca tgtgaatatc
    75301 cggcgacatc aatattcgcc gaacctataa ttacgacata atccttttcg cgcatagcac
    75361 gtcctctggt aaaaaattac caaccctgtc aatccacaag aatatagata ctgcaaagac
    75421 ggtttaaaca tgtgttcata tttaaacatg tgctcatagt atgcatgttt tacgtaaagt
    75481 aaattaccgc cagagaggca aaatgggcag atttgagcag gctcacaaaa tgcgattaaa
    75541 tgcctgaatt acaaaagcga ggaatttgtg acgggatgca cagaacagaa caataaaaaa
    75601 gggcagaaaa taatctgccc ttgaggaaat aacctgagtg tttgatgaac gtttgaagtt
    75661 tctactgaaa gataaaacca tttaaattca taaaattaca ttcacaaacc ggtcattctc
    75721 tttgcgctca tcaaatgtta caggacagga aatttctgcc ctgtaacaca ccttttatta
    75781 cgctgctttc gctactgcgt ccacttccgg acgtttcagg aaggcatagg ccaaacccgc
    75841 caccagcgta ccggcaataa ttgctaccag gtaacccaat accggcgtaa tagcgccagg
    75901 gatcagcaga acaaacagac caccgtgcgg tgccatcagt ttcgcaccaa tcgccattga
    75961 gattgcgcca gtcagcgccc cacccacgat acagcacggc agcacacgca tcggatcacg
    76021 agcagcaaac ggaattgcac cttccgaaat gaagcacagt cccaatacca gagcggcttt
    76081 gccaccttcc tgctgcgctt tgtcgaattt gcgacgcgcc accattgttg ccagacccat
    76141 tgccagcggt ggcaccatac ctgccgccat aatcgccgcc atcgggccat aggtttgagt
    76201 actcagcaga cccacaccga atgcgtacgc tgctttgttt accggaccgc ccatgtcagt
    76261 acacatcatg ccaccgagga tcgcccccag cagaaccgca ttcgcagtcc ccatggtctg
    76321 cagccagtga gtcagccctt cgagaatgcc agcaactggt ttaccgatca ggtagatcat
    76381 cgccagaccg accaccagac tggaaattag cggaatgatc aggatcggtt tcagcgcctc
    76441 catactctgt ggcagtttca attgcgtact gattaacttc gcaatgtaac cagccaggaa
    76501 gcccgcaata ataccaccaa tgaagccaga accggtgctg accgccagca taccgccaat
    76561 cagacccgga gtgaggcccg gacgatcggc aatggaaaag gcaatataac ctgccagtac
    76621 cggcaccatc agcgcaaagg ctgaaccacc accaatctgc atcagcgccg cagccaacgt
    76681 acccggctct ttaaacgctt cgataccaaa agcaaaagaa agcgcgatac acagaccacc
    76741 tgcaacgacc atcggcagca tgtaagaaac gcccgtcagc aagtgacggt atgcgcctgc
    76801 actctctttc ttactttcag tggtcgccgt ttgagctttg cccgccggtt catacggcgt
    76861 tgcttcagca accgctttat ccagttcctg cgcggttttc ttcagcgcca gaccggtaga
    76921 ggtacgatac atcggtttac cagcaaattt cgccagatcc acttcgatat ctgccgccac
    76981 aatcaccaga tccgctgctg cgacttcttc gggagtgatt gcattacccg cgccaacaga
    77041 accacgggtt tcaactttca cccaccagcc acgttttttc gcttcggttt caatggcttc
    77101 agccgccata aaggtgtgtg ctacgccagt cgggcaagca gtcaccgcaa ctacgcgttt
    77161 cggaccgctg gcggcaactg gtgctgtcgc agcgaccggc gcagtgtaag gtttcgcatg
    77221 gcctttggct tcactcagga acagctcagg gtgcgcaact gcccgggaaa tatcgcccag
    77281 ccagacattt ttaccgttca gcgcgctgtc gttcgggatg gaatcaccga gaacaatcgc
    77341 catttcagcg tcgttcggat tgtcgatgat ttccagtttt gcttttcgcg ccgccgcgcc
    77401 cagcagggtc ttcgccatat aggcgcgtgc ctgaccgaga ttagcgtcaa taatcagcag
    77461 cgttttcatt atgcctctcc tgctgtcagt taaaaggttg taagtcgacg cgcgccatca
    77521 ttgcggccaa ctgcggacga tcggtaatac ccacattgct ttgacttacc gccagggctg
    77581 caacagctgt cgccagacgc agtgtgtgtt cactggattc acgcatcagc aagccataaa
    77641 tcaggccacc aaccatagaa tcccctgcgc caacggtgct tacgacatcg actgacggtg
    77701 gtttggcgat ccattcgccg gaggcattaa cccaaagcgc gccttcggca cccagtgaaa
    77761 taacaacatg cgcgatgcct tgttcacgta gcgcatgtgc agcttcaatc acatctttca
    77821 tttcaggcag tttacggcct gcccagattt ccagctcgcg gcggttaggt ttcaccagcc
    77881 acggtgccgc tttcaaacct gctactaacg cttcacggct actatcaaag ataatgcaag
    77941 gacactgact acgcaggcga gtcatccagt cggtgaacgc ttccgggctg acgcctgacg
    78001 gtaagcttcc gctgacacag accatatcga actgaccgag ccagctcaga gaatcagtca
    78061 caaagcgttc ccagtcggcg ggggtgactt caaaacccga gaagttgaag tcggtcactt
    78121 cgccgtcttt ttccgtcagc ttaacgttaa ttcgggtgcg cccctgtaca acctggaaac
    78181 ggttggcaat gcccagctcg ctgaacagtt gctgaaaacc atcctgattg tctttaccca
    78241 ggaagccgcc aacggtgaca tcaattccca ggtcttttaa tactttggcc acgttgatgc
    78301 ctttacccgc cgcatgcaga ccggtggttt tcaccaggtt cacttcgccg cgttcaattt
    78361 ccgggcagaa accaacaagg tcataagccg gattaagggt gatagtagca acacgtctgc
    78421 tcattatgcg ccctccccaa gaccagcagc gatagcgtcg ccgattgctt tcagcgcctg
    78481 ttcagcatct gcaccctggg cggtaaagcg taggcgatga cctttcttaa cgccaagtgc
    78541 cacaactttc atcagactac gtccgtttgc cggtttgccg gtaccatcaa ggtttgtcac
    78601 ggtaatatca ctgttaaatt gtttaatggt attgaccagc atggtacctg gacgagcatg
    78661 caggccgtgt tcattgcgca ccacaaactc cgcgcttaac acgtcgtcgg tcggcgcatc
    78721 atcgctggtc agcagcgcca gcaacgttgc cgcatccgct ttcagcaagc ggtcagcttt
    78781 attgtcgagc aataaatcag cgagacgctt aagaaccgcg atgggctgat cgtcattcat
    78841 cgccacactc accagcatgg ctgccgtttc gccgtccaca tcaaaagcat ttgccgcacg
    78901 gcttaccgca atcgcgctac gcagattgcc ttcggcgcta tcgctcagcc agataccctg
    78961 tccgagattc agcggttgtt cattgatggc tttggtgacg aaagtggcgt caactgcccc
    79021 cgcctctttc agacgcgcag cgttcagcgc ctgaagagtc agcagatcgc tggcgacgat
    79081 atccagtgtc agcatttcgt tgtcgagctt cagctgctca ctctgctttt cgcccatcag
    79141 taatgcgcga agttcttctg ctgttgttgc tgacttcagt tgttcagcaa cggaatcatc
    79201 gctcagtacg tgggtcagct ggcgtagcag gcccagatgt tcatccgagc tggcagcaat
    79261 accgattgcc acgtacgcta cctgaccgtc accccaggtg acgccttccg ggaactgaaa
    79321 tacctgaacg ccggttttca gcacctgatc gcgggtgtcg gtagtgccgt gtggaatagc
    79381 aataccattg ccgaggaacg ttgaggtttg ctgttcgcgc gccagcatgc cattgacgta
    79441 gccttctgct acattaccgg cctgcaccag cgccgcagcg acctggcgaa tcgcctcttc
    79501 tttgtctccg gccttttcgc ccggatggat gtcctgtacg gataactgga acatagttct
    79561 cctctcttgc tgaattgaaa cgattcagcc tctatgagaa aaaaagcgcc aacctggctt
    79621 agggttaaag acaagatcgc gctgaaacgt ttcaagaaag cataatactt ctgtttcagc
    79681 acgcaaggaa agccgtaaag ttatgagcaa aagtttgatg tgctgcacaa ttttttgcca
    79741 tttttcctaa ttgctgatgg gaaaatccgt tatcagcaat ttcatttcag cagcgtcagc
    79801 agccaatcag caacagtgaa atggcatttt gactattcgt ggcgcttttg atttcgctgt
    79861 ctgtttattt tctgacaagc agcgtaaact ccgcgtcttc ctcttccagt gatcgaccag
    79921 catgcataac tcccccgcag tctccagcgc gaaatcgttt gacctgacct cgacggcgtt
    79981 tttaatcgtt gcctttctca ccggtattgc gggcgctctg caaaccccga cactcagtat
    80041 ttttcttacc gatgaagtac atgcccgtcc ggcgatggtg ggattcttct ttaccggcag
    80101 cgctgtcatt gggattctgg taagtcagtt tctcgccggg cgctctgata agcgcggcga
    80161 tcgcaaatcg ctgattgtct tttgctgcct gttaggcgtg ctggcctgca ccctttttgc
    80221 ctggaatcgc aactactttg ttttgctatt cgttggcgtc tttcttagca gctttggctc
    80281 gaccgctaac ccgcaaatgt ttgcccttgc ccgtgaacat gccgacaaaa ccggacgtga
    80341 ggcggtgatg ttcagctctt ttttacgcgc tcaggtttca ctggcatggg tcattggccc
    80401 accgctggct tatgccttag cgatgggttt cagctttacg gtaatgtatc tgagcgcagc
    80461 ggtagcgttt attgtttgcg gtgtgatggt gtggctgttt ttaccgtcga tgcaaaaaga
    80521 gcttccgctg gcgaccggca cgatcgaagc gccgcgccgt aaccgtcgcg atacgctgct
    80581 gctgtttgtc atttgtacat tgatgtgggg ctcgaacagc ctgtacatca tcaacatgcc
    80641 gctatttatt atcaacgaac tgcatcttcc cgagaaactg gccggtgtga tgatggggac
    80701 cgccgccggg ctggaaatcc cgacgatgtt gattgccgga tatttcgcca aacgtctggg
    80761 taagcgtttc ttaatgcgcg ttgctgccgt gggtggcgtc tgtttttacg caggaatgct
    80821 gatggcgcat tcacctgtca ttctgttggg cttgcagctg ctaaatgcta tttttattgg
    80881 cattctgggc ggcatcggga tgctctattt tcaggatctg atgcccggtc aggcgggttc
    80941 agccaccacg ctctatacca acacttcgcg cgtgggctgg atcatcgcag gatcagtggc
    81001 gggcatcgtc gccgagatct ggaattatca cgctgtgttc tggtttgcga tggtgatgat
    81061 tatcgccact ctgttttgct tactgcggat taaagatgtt taaggcgcgg tcagcatttc
    81121 cagatcgata agccaggtca tggcctgctg acgcgagtta ccgcacattt cagcgctggc
    81181 ttgtaagcct gcacagactt tcggtcgcag tggcgaggtg aaaattttac agcgttgctg
    81241 ttcatcaagc tgaatgcagg gcgtattggc gggcttgcca tctggcatac cgggaatagg
    81301 gctggaaatt gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt ccatacgttg
    81361 ttctcttaag gttaaccacc ggattgatga gcgcgcacag taccaccttt tttgcaccag
    81421 caaaagtgcg aataccactt gcccgaaagg cccgtcgcga gtactttgtc gcgatatttt
    81481 tgacattttc gactacagga atttttcgat gccaagagcg aacgaaatta aaaaaggtat
    81541 ggtactgaat tacaacggca aactgctgct ggttaaggat attgatattc agtcgcccac
    81601 tgcccgcggt gccgctacgc tgtacaaaat gcgtttttct gatgtccgta ccgggctgaa
    81661 agtagaagag cgtttcaaag gtgatgatat cgttgacacc gtgacgctga cccgccgtta
    81721 cgttgacttc tcctatgtcg atggcaacga atatgtcttt atggataaag aagactatac
    81781 cccgtatacc ttcaccaaag atcagattga agaagagttg ctgtttatgc cagagggcgg
    81841 catgccggac atgcaggtgc tgacctggga cggtcaactg ctggcgcttg agctgccgca
    81901 gaccgttgat ctggaaatcg ttgaaaccgc accaggcatc aaaggggcat ctgccagcgc
    81961 ccgtaacaaa ccggcgacat tgagcactgg tctggtgatt caggtaccag aatacttaag
    82021 cccgggcgaa aaaattcgta tccatatcga agaacgccgt tatatggggc gtgctgacta
    82081 acttcagccg catgcagaaa agggatagct caggctgtcc cttttttaat ttattatacc
    82141 aatcttctat tttgcgcttc ctgaacacct tacgccctca attttcactc gttgattgat
    82201 cgccctcaca cttcatcgca ttaacaatcc agaccaattt caattgctgt catataactt
    82261 tacactgtcg ttgttaatta atcgttacta agacgtgact cctatgaata caatcgcctc
    82321 cgttacgctc ccgcatcatg tacacgctcc acgctatgat cgccagcagt tgcaatcacg
    82381 tatcgttcat tttggctttg gagcctttca ccgcgctcat caggcgttac tgaccgatcg
    82441 tgtgctgaat gcccagggcg gcgactgggg gatctgtgaa atcagcttgt tcagcggtga
    82501 tcaactgatg agccagctcc gcgcacagaa ccatttatat accgtgctgg agaaaggtgc
    82561 ggacggcaat caggtgataa ttgtcggtgc cgttcacgaa tgccttaatg caaaactgga
    82621 ttccttagcg gcaattattg agaaattttg cgagccacag gtggcaattg tttccctgac
    82681 gattaccgaa aaaggctatt gtattgaccc ggccaccggt gcactcgaca ccagtaatcc
    82741 gcggattatt cacgatctac aaacccctga agaacctcac tccgcaccgg gtattctcgt
    82801 cgaagcactg aaacgccgcc gtgagcgcgg ccttacaccg tttaccgtgc tctcctgcga
    82861 caatattccc gacaatggtc atgtggtgaa aaacgcggtg ctgggaatgg cagaaaaacg
    82921 ttcgccagaa ctcgccgggt ggataaaaga gcacgtcagt tttccgggaa ccatggtcga
    82981 ccgcattgtt ccggctgcaa ccgacgaatc actggtggaa atcagccagc atctgggggt
    83041 gaatgatccc tgcgcgatta gctgcgaacc gtttatccag tgggtggtgg aagataactt
    83101 cgtcgctggg cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg atgtcctgcc
    83161 atgggaagag atgaaactgc ggatgcttaa tggcagccac tcttttctcg cttatctggg
    83221 ttacctctca ggattcgccc atatcagtga ttgcatgcag gatcgcgcat ttcgccatgc
    83281 cgccagaaca ttaatgctgg atgagcaagc gccgacactg caaattaaag atgtcgattt
    83341 aacacaatat gcggataagt taattgcacg ttttgctaat ccggcgctga aacataagac
    83401 ctggcaaatc gcgatggatg gcagccagaa attaccgcaa cgcatgctgg caggtattcg
    83461 catacatcag gggcgcgaaa cggactggtc gttgctggca ttaggcgttg caggctggat
    83521 gcgttacgtc agcggcgttg atgatgccgg aaatgccatt gatgttcgcg atccgcttag
    83581 cgataaaatt cgcgaacttg ttgcgggcag cagcagtgaa caacgcgtaa ccgccctgct
    83641 ttccctgcgt gaagttttcg gtgatgatct gccagataac ccgcattttg tgcaggccat
    83701 cgaacaagcc tggcaacaaa tcgtacaatt cggcgcacat caggcgctat taaacaccct
    83761 caaaatttaa cgatttctgc ggttaaagcg gatgaagctc accttcgtcc gctctcccct
    83821 tctcttttct gcctttttta gccaggatta acgctcagtt aacttaccag agtattgcgg
    83881 agccgttgtg accaggacca acctcatcac cggttttctc ggcagcggga aaaccacgtc
    83941 gattcttcat ctgttagccc ataaagatcc caacgaaaaa tgggcggtac tggttaatga
    84001 atttggggaa gtcggaattg atggtgcttt gctcgccgat agcggcgcat tgctgaaaga
    84061 gatccccggc ggctgcatgt gctgcgttaa tggtttaccc atgcaggtag ggttgaatac
    84121 cttactgcgt cagggaaaac cagaccgctt gttgatagag ccgaccgggc tgggccatcc
    84181 gaaacagatc ctcgatctgt taaccgcacc agtctatgaa ccgtggatag atctgcgcgc
    84241 caccttgtgc attctcgatc cgcgcttgct gctggacgaa aaaagcgcca gcaatgaaaa
    84301 cttccgtgac cagctggctg ccgcagacat cattgtcgcc aataaatccg accgtacgac
    84361 gcccgaaagt gagcaagcgc tacagcgttg gtggcagcaa aatggtggcg atcgacaatt
    84421 aattcacagt gagcatggga aagttgacgg tcatcttctg gatttgccgc gtcgcaattt
    84481 agccgagttg cccgccagcg ccgcgcattc tcatcagcat gtcgtgaaaa aagggttagc
    84541 agcgttaagc ctgccagagc atcaacgctg gcgtcgcagt ctgaacagcg ggcaaggata
    84601 tcaggcctgc ggctggatat tcgacgctga tacggtattc gacaccattg gcattctgga
    84661 atgggcgcga cttgcaccgg tggaacgcgt caaaggcgtg ctgcgtattc ccgaagggct
    84721 ggtgcgaatc aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg ttgcgccacc
    84781 ggacagccgt attgagctga tttccagcag cgaagctgac tggaatgcct tgcagagcgc
    84841 gctgttgaag cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt cagtaagata
    84901 attagagaaa atatgattaa aaatttgccg caaatagtgt tgttgaatat tgtcggcctc
    84961 gcgctgtttc tttcctggta tatccccgtt aatcatggat tctggttgcc gattgatgcg
    85021 gatatttttt atttctttaa tcagaaactg gtcgaaagta aggccttttt gtggctggtt
    85081 gcattgacca acaatcgcgc cttcgacggt tgttcactgc tggcgatggg tatgttgatg
    85141 ctgagtttct ggctgaaaga aaacgcccct ggcagacgac gtatcgtgat tattggtctg
    85201 gtcatgctat taactgcagt ggtattaaac cagctgggtc aggcattaat tcctgtaaaa
    85261 cgggccagcc caacattgac ttttaccgat attaaccgcg tcagcgaact gctctctgtt
    85321 cccacgaaag atgcctcacg agatagcttt cccggcgatc acggcatgat gctgcttatt
    85381 ttttcggcat tcatgtggcg ttatttcggc aaagttgcag gccttatcgc ccttattatt
    85441 tttgtggttt ttgcatttcc cagagtaatg attggcgcac actggtttac tgacatcatt
    85501 gtcggttcga tgaccgtgat attgatcggt ttgccctggg tgttgctgac gccattaagt
    85561 gatcgattaa tcaccttttt tgacaaatca ctaccaggaa aaaacaaaca tttccaaaac
    85621 aaataactca cagtaattaa catcatcagg gttattttta tagtgaggat aatcctgatg
    85681 atgcgcaccg tgctttcatc tatcgaacgc aaaaatcatt ctctaagtaa atgaatggat
    85741 tgcatgcgtt tcactcaatt gtactttaat tgaccaaccc cgcttattaa ctttctgtat
    85801 cactttttct tataaaaaat catgtaaaac cgctcgccaa gaccgcacca atcgggtaat
    85861 ctcgaactcg ttttgcctcg gcggtagatt atcctcacag catataattt tgtgcgttag
    85921 tccacagatt tggccttaag gaattgtttc aacatgccca ggtaattagt ctcgtgtcgc
    85981 ttggcatttt tttataacga tatttgtcgt taaggacttc aagggaaaac aaacaacatg
    86041 gtcaaatctc aaccgatttt gagatatatc ttgcgcggga ttcccgcgat tgcagtagcg
    86101 gttctgcttt ctgcatgtag tgcaaataac accgcaaaga atatgcatcc tgagacacgt
    86161 gcagtgggta gtgaaacatc atcactgcaa gcttctcagg atgaatttga aaacctggtt
    86221 cgtaatgtcg acgtaaaatc gcgaattatg gatcagtatg ctgactggaa aggcgtacgt
    86281 tatcgtctgg gcggcagcac taaaaaaggt atcgattgtt ctggtttcgt acagcgtaca
    86341 ttccgtgagc aatttggctt agaacttccg cgttcgactt acgaacagca ggaaatgggt
    86401 aaatctgttt cccgcagtaa tttgcgtacg ggtgatttag ttctgttccg tgccggttca
    86461 acgggacgcc atgtcggtat ttatatcggc aacaatcagt ttgtccatgc ttccaccagc
    86521 agtggtgtta ttatttccag catgaatgaa ccgtactgga agaagcgtta caacgaagca
    86581 cgccgggttc tcagccgcag ctaataaacc gtttggatgc aatcccttgg ctatcctgac
    86641 gagttaactg aaagcactgc ttaggcagtg cttttttgtt ttcattcatc agagaaaatg
    86701 atgtttccgc gtcttgatcc aggctatagt ccggtcattg ttatctttta aatgttgtcg
    86761 taatttcagg aaattaacgg aatcatgttc atacgcgctc ccaattttgg acgtaagctc
    86821 ctgcttacct gcattgttgc aggcgtaatg attgcgatac tggtgagttg ccttcagttt
    86881 ttagtggcct ggcataagca cgaagtcaaa tacgacacac tgattaccga cgtacaaaag
    86941 tatctcgata cctattttgc cgacctgaaa tccactactg accggctcca gccgctgacc
    87001 ttagatacct gccagcaagc taaccccgaa ctgaccgccc gcgcagcgtt tagcatgaat
    87061 gtccgaacgt ttgtgctggt gaaagataaa aaaacattct gttcatctgc gaccggtgag
    87121 atggacattc cactcaatga attgattccg gcgctcgaca ttaataaaaa cgtcgatatg
    87181 gcgatcttac ccggcacgcc gatggtgccg aacaaacccg caatcgtcat ctggtatcgc
    87241 aaccctttgc tgaaaaatag cggcgtcttt gccgctctga atctcaacct gacgccttca
    87301 ctcttttata gttcacggca ggaagattac gatggcgtcg ccctcattat tggcaatact
    87361 gcgctatcta ccttttcttc acgtttgatg aacgttaacg aattaaccga catgccagtc
    87421 cgtgaaacta aaattgcggg cattcctctg accgttcggc tttatgcaga tgactggaca
    87481 tggaacgatg tgtggtacgc atttttactg ggcggcatga gtggaactgt cgttggcctg
    87541 ctctgctatt acctgatgag cgtacgtatg cgccccggca gagaaatcat gaccgccatc
    87601 aagcgcgaac aattttacgt ggcgtatcaa ccggtggtgg atacacaagc tttgcgagta
    87661 acgggcctgg aagtactgct acgctggcgg catcctgtcg cgggagaaat tcccccggat
    87721 gccttcatta actttgccga atcgcaaaag atgattgtgc cgctgactca gcacctgttt
    87781 gagttaattg cccgcgatgc cgcagaatta gaaaaagtgc tgccggtagg cgtcaaattt
    87841 ggtattaaca ttgcgccgga ccatctgcac agcgaaagct ttaaagcaga tatccagaaa
    87901 ctgctcactt ccctgcccgc acaccatttc cagattgtgc tggaaattac cgagcgcgat
    87961 atgttgaaag agcaagaagc cacacaactc ttcgcctggc tgcactcggt cggcgtagaa
    88021 attgctattg atgacttcgg caccgggcac agcgcgctta tctatcttga gcgttttacg
    88081 ctcgattatc tgaaaattga ccgtggattt atcaacgcca tcggtacgga aacgatcact
    88141 tcccccgtac ttgacgcggt gctgacgctg gcgaaacgcc tcaatatgct gacggttgct
    88201 gaaggggtcg aaacgccgga acaggcgcga tggctaagcg aacgcggcgt taatttcatg
    88261 caaggctact ggattagccg cccgttaccg ctggacgatt ttgttcgctg gctaaagaaa
    88321 ccgtatacgc cgcagtggta aggtgtgctt acgtccctta ttattcatag tgaaagcatg
    88381 ccggattgcg gctaatgatg agtaaaagga aatccgttgc agatgattgt gcgcatactg
    88441 ctgctgttta tcgctctgtt cacctttggt gtgcaggcgc aggctatcaa ggaaagctat
    88501 gcctttgccg tgctgggcga accccggtac gcgtttaatt tcaaccattt tgattatgtg
    88561 aaccccgccg cgccaaaagg tgggcagata acgttgtcag ccctcggcac cttcgataat
    88621 ttcaaccgct atgcactgcg cggcaacccg ggcgcacgca ccgagcagct gtacgacacg
    88681 ctatttacga cttccgatga cgaaccaggc agttattacc cgctgattgc tgaaagcgca
    88741 cgctatgctg acgattattc ctgggtggag gtcgctatta atccgcgcgc ccgttttcat
    88801 gatggttcgc ccattactgc ccgcgatgta gagtttactt ttcaaaaatt tatgaccgaa
    88861 ggcgtgccgc aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat tgcaccgtta
    88921 accgtgcgca ttgagttagc taaacccggc aaagaagata tgctgagtct gttttcgctg
    88981 ccggtatttc cagaaaagta ctggaaggat cacaaactta gcgacccgct cgccacgcct
    89041 ccgcttgcca gtggtccgta ccgcgttacg tcctggaaaa tggggcaaaa tattgtctat
    89101 tcccgtgtga aagattactg ggcagcaaac ttaccggtaa accgtggacg ctggaatttc
    89161 gacaccattc gctacgatta ttacctcgat gataatgtcg cctttgaagc gtttaaagca
    89221 ggtgcctttg atttgcgtat ggaaaacgac gccaaaaact gggccacgcg ttataccggt
    89281 aaaaatttcg ataaaaaata catcatcaaa gatgagcaaa agaacgaatc agcccaggat
    89341 acgcgttggc tggcgtttaa tatccaacgt ccggtattca gcgatcgccg ggtccgggaa
    89401 gctatcactc tcgcctttga ctttgaatgg atgaacaagg cgttgtttta caatgcctgg
    89461 agtcgcacga acagttattt tcagaatacc gaatacgcgg ccagaaatta ccccgacgcc
    89521 gcggagctgg tgcttctggc accaatgaaa aaagatctac cgtcagaagt cttcacacaa
    89581 atctaccagc cgccggtatc caaaggcgat ggctacgatc gtgacaacct gttaaaagcc
    89641 gacaaacttc tcaacgaagc gggctgggtg ctgaagggtc agcaacgcgt taatgccaca
    89701 acgggtcagc cactcagctt tgaattattg cttcccgcaa gcagcaatag tcagtgggta
    89761 ttgccgttcc agcacagcct gcaacggctg ggtatcaaca tggacattcg caaggtggat
    89821 aactcgcaaa tcactaaccg catgcgcagt cgcgactatg acatgatgcc gcgcgtatgg
    89881 cgggcgatgc cgtggcccag ttccgattta cagatttcct ggtcatcgga atatatcaat
    89941 tccacttata atgcccccgg cgtgcaaagc ccggttatcg actcgctgat caaccaaatt
    90001 attaccgcgc agggaaataa agaaaaatta ctgccgttgg ggcgagcact ggatcgcgta
    90061 ttaacgtgga attattacat gctgccaatg tggtacatgg cggaagaccg tctcgcctgg
    90121 tgggataaat tctcccagcc ggccgtgcgc cccatctata gcctcggtat cgatacctgg
    90181 tggtatgacg tcaataaagc ggccaaactg ccgtccgcca gcaaacaggg agagtagatg
    90241 ggcgcttacc tgattcgccg tctgttgctg gtgatcccaa cattatgggc gattatcacc
    90301 atcaactttt tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc catcgccgcc
    90361 attgagtttg gtaatgccgg agtattaccc ggcgcaggcg gtgaaggtgt tcgtgccagc
    90421 catgcgcaaa cgggtgtcgg caatatcagc gacagtaatt accgtggcgg acgcggatta
    90481 gatccagaag tgatcgctga gatcactcat cgctacggtt ttgataagcc gatccacgaa
    90541 cgttacttca aaatgctctg ggactacatc cgctttgatt ttggcgatag cctgtttcgc
    90601 agcgcctcgg tgctgacgct gattaaagac agtctgccgg tttccatcac cctcggattg
    90661 tggagcacgc tgattatcta tctggtgtcg attccgttag gcattcgcaa agctgtttat
    90721 aatgggagcc gctttgacgt ctggagtagc gcatttatca tcatcggcta cgccattccg
    90781 gcctttttgt ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta tttcgacctg
    90841 ttccctctac gcggcctggt ttccgctaac tttgattcgc tgccgtggta tcagaaaatc
    90901 accgattatc tgtggcatat cacgctgccg gtgctggcga cagtgattgg tggctttgcg
    90961 gcgctgacca tgctgacaaa aaactcattc cttgatgaag tgcgcaagca atacgtggtg
    91021 accgcgcgtg cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt gttccgcaac
    91081 gccatgctgc tggtgattgc cggttttccg gcgacgttta tcagcatgtt ttttaccggc
    91141 tcgctgctga ttgaggtgat gttttcactc aatggtctgg gcttactggc tacgaagcga
    91201 ccgtctcgcg cgattatcct gtaatgtttg gtaccttgta tattttcacc ctgattggcc
    91261 tgctgctgaa tattgtcagt gatatcagct atacgctggt tgatccgcgt attgattttg
    91321 agggacgtta atgtcgcgac tcagccccgt caatcaggcc cgttgggcgc gttttcgtca
    91381 taaccgtcgc ggctactggt cgttatggat tttcctcgtc ttgtttggtt tgagtttgtg
    91441 ttctgaactt atcgccaacg ataaaccgtt gctggtgcgt tatgacggca gttggtattt
    91501 cccgttattg aaaaactaca gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga
    91561 ttatcaggac ccgtggctga aacaacggct ggaaaataac ggctgggtac tgtgggcacc
    91621 gattcgcttt ggtgctacca gtatcaactt tgctaccgat aagcccttcc cttctccacc
    91681 ctcccggcaa aactggctgg gaacggatgc caacggcggc gatgtgctgg cacgcattct
    91741 ctatggcacg cggatctcgg ttctgtttgg cctgatgctg actctctgtt ccagcgtgat
    91801 gggcgtgctg gcgggggcgc tacaaggcta ttacggcggt aaagtcgatc tctggggaca
    91861 acgctttatt gaagtatggt cggggatgcc gacgctgttt ttgattattt tactttccag
    91921 cgtcgtacag cctaacttct ggtggctgct ggcaattact gtcttgtttg gctggatgag
    91981 tctggtcggc gtggtgcggg cggagttttt acgtactcgt aatttcgact acattcgtgc
    92041 ggcacaggcg cttggcgtca gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc
    92101 catggtcgcg accctcacct ttttaccgtt tattttatgt agttcgataa ccaccctgac
    92161 ctcgctcgat ttcctcggct tcggtctacc gctcggttca ccgtcactcg gtgaactgct
    92221 gttacaaggg aaaaataacc ttcaggcccc gtggcttggg atcaccgcct tcttgtcggt
    92281 ggcgatatta ttgtctttgc tgatctttat tggtgaagcc gtccgcgacg catttgatcc
    92341 taataaggcg gtgtagcatg acgcaaactc tgttagcgat tgaaaatttg tcggtgggtt
    92401 ttcgccatca gcaaaccgta cgtacagtag tcaatgatgt ttcactacag attgaggctg
    92461 gcgaaacgct ggcgctggtg ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa
    92521 ttttacgcct gctcccttcc ccgccggttg aatatctctc cggcgatatt cgttttcatg
    92581 gcgaatcgct gcttcacgcc agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg
    92641 ccatgatttt tcaggaaccg atggtgtcgt taaatccatt gcataccctg gaaaaacagc
    92701 tttatgaagt gctttcactc caccgcggga tgcgtcggga agcggctcgt ggcgaaattc
    92761 ttaactgcct tgatcgcgtt ggtatccgcc aggcggcaaa acggctgaca gattatccgc
    92821 atcagctctc cggcggcgaa cggcagcggg tgatgattgc gatggcgctg ttaacgcgac
    92881 cggaattatt aattgccgat gaaccgacca ccgcactgga cgtctctgtc caggcgcaga
    92941 ttttacagct gttgcgcgaa ctgcaaggcg agctgaatat gggcatgctg tttattactc
    93001 ataacctcag cattgtcaga aaactggccc accgcgtggc ggtaatgcaa aacggtcgct
    93061 gtgtcgagca aaattacgcc gctacgctat ttgcatcacc cactcatcct tacacacaaa
    93121 agctactcaa cagtgaaccg tcaggcgatc cagtgccgtt gccagaacct gcctcaacgt
    93181 tgctggatgt tgaacagctt caggttgcct tccccattcg caaagggatt ttgaagcgca
    93241 ttgtggatca taatgtggtg gtgaaaaaca tcagttttac gctacgagcg ggtgaaacac
    93301 tgggtttagt gggcgagtcc ggttccggga aaagtacgac gggactggcg ctgctgcgac
    93361 tgattaattc tcagggcagc atcatctttg acggtcagcc actgcaaaat ttaaatcgcc
    93421 gccagctgtt acctattcgt catcgcattc aggtggtatt tcaggatcca aactcctcgc
    93481 tcaacccacg actcaacgtt ttgcagatta ttgaggaagg cttacgggtt caccagccga
    93541 cgctttctgc cgcacaacgc gaacaacaag tgatagccgt gatgcatgaa gtgggattag
    93601 atcctgaaac acgccaccgt tatccggcgg agttctctgg tggtcagcga caacgtattg
    93661 cgattgccag ggcattaatt cttaagccct cgctgatcat acttgatgaa ccgacatcat
    93721 cactcgacaa aacggtacag gcgcaaatat tgacgctatt gaaatcattg caacaaaagc
    93781 atcaactggc ctatttgttt atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc
    93841 aggttatcat actgcgacaa ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg
    93901 ccacaccgca gcaggagtat acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga
    93961 ttgtagtttg aaaagggttc ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca
    94021 gcaaactcgt cctggcgatt tacaaacagg cacggctcac cttcacactc cagcaccgaa
    94081 catggaactt cgatgtcgct taacgcctgg cttagtttat acatcaccga ccatgcatta
    94141 tcaccatccg ggctaagcaa tttaagcgcc accaggctgt tgtcaccgca cggtccattt
    94201 tgcggaatcg gttcaacctt cgaacctgcg aaaccagcag accagcgata gttctgcggc
    94261 agtcgatgga cgattgagag ttgtaatgaa gtcacttatt ttccccggaa gcacattcac
    94321 ttcacaattt gtttacatca attttaacac atcatcaaca atccgtcttt atacagatcg
    94381 taaagattga tgcctcgcct gcgtggcctc tatggctctg atttaagtaa tatctgcggg
    94441 cgtggtcacg tttgcggggc tatatgtacc cgtttctgcg atctaactca acctttttaa
    94501 ctacaatgat gtgacttttt acataaattg attttacata aaataaacat atatcggggg
    94561 aatgatagat ttgtggttga catgcatcaa caaaggaagc cttttagctt cctcgttgtg
    94621 caatagatca ccgttttttc ggccgactgg cgtacagaca gaagagaatg gagctggttg
    94681 cgcagaatgc aattgaccaa atcatcggcc atgcagagtt aaaggtcgca agagaaagca
    94741 atgcgccaac aattgcccct atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc
    94801 ccgccatatg gggaaactca tcaagaatga ccgccatcgc attggatgac accatcgaca
    94861 cgcagcccac aaacgccgca acgccaacca ccagcgacca aaatcccagc cccagcagcg
    94921 cactgatgac catccacgct gccataataa attgtatcca caaccccgag cggaacatat
    94981 ttaacgcgcc aatgcggcgg acgaagcggc tgttaaagat ggtcatcacg aacagaaaaa
    95041 caatgtttag cgcaaagtaa taaccaaagt tttccggcgc gacgtggtta atttcaatat
    95101 aaacaaacgg tccggcgctt aagaatgaga acatcccggc aaagctgaaa ccactggcaa
    95161 gcatgtagct caggacacgt ttatggcgga acagcgccgc aaagttacca atagtggtac
    95221 gaatgtgaaa tggctgacga cgctccggtg gtaaggtttc tttaatcagg aagaaaatca
    95281 ttgccgaagc cagaatcgcc gctaatgcca ggatccagaa gatgtaatgc cagctcagcc
    95341 acaccagcac ccagccgcca actatcggtg ccatcagcgg tgcaatggtt gtcaccagca
    95401 tgacaaacga catcatccgc gagaactctt ctttcgggta aatatcgcgc atcagggcgt
    95461 taatgaccac gctggccgca gccgcagcca gcccgtggaa gaaacgcatc acaatcagct
    95521 gatcgatggt gtttgccaac gcacacgcca ccgcggcggc ggcaaacacc agcgtaccgc
    95581 cgagcaccac cggcttacgc ccgaagctgt ctgccatcgg cccgtagatt aactgcccca
    95641 acgcaaagcc cagaatataa gtactgaggg tcatctgcgt actgcccgcc ggtacgccaa
    95701 actgcgctga aattaccggt agcgcgggca gatacatatc aatcgacagc ggcatcaaca
    95761 tggccagcag gccaaggata aaaacaatag caaacgacga atgctgtcgg gtggtcacaa
    95821 cgggctcctg aaagtcattg aaaagttaga cgacgctggc aatttcttct tcagttaacg
    95881 gacgatattc accgggggct aaatcagcat ccagcgtaat accgccaata cgttcacgat
    95941 gcagctcaac cacgtggtta cccacggcgg cgaacatgcg tttcacctga tgataacgcc
    96001 cttcgctgat ggtcagacga acctgcgttg gggtaatcac ttccagcacc gcaggcttag
    96061 tgagatcttt ttcgttatgc agctgcacgc ctttagcaaa ttgctctgcc gtatcgtcag
    96121 ctacaggtga ttccagtgtc accagatagg tcttctcgca atgatggcgc ggagaagtaa
    96181 tgcggtgcga ccactgacca tcatcagtca tcagcaccag accggtggta tcaatatcca
    96241 accgccccgc cgcatgcagt ttccacgcta ccggttcatc aagaaaatag agcaccgttg
    96301 ggtgatcagg gtcgtccgtg gagcaaacat agccctgagg cttattgagc atgaagtaac
    96361 gtggaccgtg ttgctgcgcc agcgggttgc catcgtaagc gacatcatgt tcaggaagca
    96421 gtttgaacgc tgcattacgg acgatttcgc catcgacggt gacacgattg ccgcggattt
    96481 cacgcccggc aatagcacgg ctaacgccga gttgctgtgc gataaattta tcaagtcgca
    96541 tgtgtgtgat tttgcctgta aaaacggagg tcgggcattg acccgaaaat ctgaactgtt
    96601 gtctgcccag tatagcggtc taataacatc cctcaaggga aaaagatcca tggcatacta
    96661 ttagcagaat aatctaccta cgcgagacca tgatttttac acttcgccca tatcagcaag
    96721 aagccgtgga tgccacgctc aaccattttc gtcgtcataa aacccctgcc gttatcgtgc
    96781 tgcccaccgg cgcaggtaaa agcctggtga tagcggaact ggcacggctg gctcgtggtc
    96841 gcgtgctggt gctggcacac gttaaagaac tggtggcgca aaaccatgca aagtatcagg
    96901 cgctggggct ggaagccgat atttttgccg ccgggctaaa gcgcaaagag agccacggta
    96961 aagtggtatt tggcagcgtg cagtctgtcg cccgtaatct tgatgccttt cagggtgaat
    97021 tttcgctgtt gattgtcgat gaatgtcacc gtattggtga cgatgaagag agccagtatc
    97081 agcaaatcct cactcacctg acaaaagtga atccccactt acgcctgctg gggctgactg
    97141 ccacgccttt tcgattgggc aaaggctgga tctaccagtt tcattatcac ggcatggtac
    97201 gcggcgatga gaaagccctt ttccgtgact gcatttatga gctgccgctg cgttatatga
    97261 ttaaacacgg ctatctgacg ccgccagaac gactggatat gccagtagtg caatacgatt
    97321 tcagccgctt gcaggcacag agtaacgggc tgttcagcga agccgatctc aaccgtgagc
    97381 tgaaaaaaca acaacgtatt accccgcaca tcatcagcca gattatggag tttgctgcaa
    97441 cgcgcaaagg ggtgatgatt tttgccgcga cggttgaaca cgcaaaagag attgtgggat
    97501 tactgcctgc cgaagatgca gcactgatta ctggcgacac ccccggcgct gagcgcgatg
    97561 tgttaattga aaattttaaa gcccagcgtt ttcgctatct ggtcaacgtc gcggtactga
    97621 ccaccggatt tgacgccccg cacgtcgatc ttatcgccat tctgcgccct accgaatcag
    97681 tgagtcttta ccaacaaatt gtcgggcgcg gtctgcgtct cgctccgggc aagactgatt
    97741 gcttaattct tgattatgcg ggtaatcctc acgatctcta cgcgccggaa gttggtacac
    97801 caaaaggcaa aagtgacaac gttccggtac aggttttctg ccctgcctgc ggttttgcca
    97861 acaccttttg ggggaaaacg accgccgacg ggacattgat tgaacacttt ggtcgtcgct
    97921 gtcagggatg gtttgaagat gacgacggtc atcgcgaaca atgtgacttc cgtttccgtt
    97981 ttaaaaattg cccgcaatgt aacgcggaaa acgatattgc cgcccgccgc tgccgcgaat
    98041 gtgacaccgt actggttgat ccggacgata tgttaaaagc ggcgctacga ctgaaagacg
    98101 cgctggtatt acgctgtagc ggcatgtctt tgcaacatgg gcacgacgag aaaggcgaat
    98161 ggttgaaaat cacctattac gatgaagacg gcgcggatgt gagtgagcgt ttccgtctgc
    98221 aaacacctgc ccagcgtacc gccttcgagc agctttttat ccgcccgcat acgcgcacac
    98281 cgggcatccc gctgcgctgg atcaccgccg ccgatatcct cgcccagcaa gccttattgc
    98341 gacacccgga ttttgtcgtc gcccgcatga aaggccagta ctggcaggtg cgtgaaaaag
    98401 tgttcgatta cgaaggtcgt tttcgtctgg cgcacgaatt acgcggttaa tggtagtttt
    98461 gattgatgta taaggcgatt gagtatagaa tctcgcccgc ttttgcatac gcaaagcaga
    98521 tcacttacct gttgctgggt cgcctgtagc aggattaatt taagagagaa agaaatgttt
    98581 actatcaacg cagaagtacg taaagagcag ggtaagggtg cgagccgccg cctgcgtgcc
    98641 gctaacaagt tcccggcaat catctacggt ggcaaagaag cgccgctggc tatcgagctg
    98701 gatcacgaca aagtcatgaa catgcaagct aaagctgaat tctacagcga agttctgacc
    98761 atcgttgttg acggtaaaga aatcaaagtt aaagctcagg acgtacagcg tcacccgtac
    98821 aaaccgaagc tgcagcacat cgacttcgtt cgcgcttaat tgctgaataa gttgtaaaaa
    98881 accccgctcc ggcggggttt tttgtatctg cagattatgc ctgattacgg tattgctatt
    98941 ttttgcaggc cagataaggc gttcacgccg catctggcat tcagaacaca aaaccgatta
    99001 attgccgcca gacgtccggc gctgcaattg gtcgcgcaaa ttcggcggcg tgcctttaat
    99061 ggtcaaggta tcggttgctg gatcccagaa gatgcgttca cctaacagca tcgcatcaaa
    99121 gttaatagtc aagccaccgc cgctaccggc aaacttcgtc aactgacgca gtgtgctacg
    99181 gtctgccggg aagctctctt ccagttcgta gcctttttca gccgcaaact ccgtgaagct
    99241 cacttcgctt acgcctgcca gctctttcga cagcgatttc agttcgatct cttcgcctgc
    99301 ctgcagctgc tcattacagt agctataaac ctgctggcgc acgttctgtc gctctgcttt
    99361 atccagttgt gcttcggcag tgaaatcatc aaccgcttgc aacagcccac ggttttgcgc
    99421 tttcgcgttt aacccttcgc tggccccgag gaaatccata aagaaatccg ccactttgcg
    99481 ccctactcgc cctttcagga aagtgagata gcgggtggac tctggattgg tttcccattc
    99541 ggttaaatcg atacgcgcaa caatatccgc atgattgata tcaagataat gggttgggtt
    99601 gatgtcgaga ttttcgttaa cgcgcatact gctcaggttg ctcagaactg ccaccagcaa
    99661 atactccacc gccagatagc gatagtggca aaacagcacg aatccaccgt cagcaaaagg
    99721 atatttcgct aattcgtcac gcaggcggcc ggttgccgcg cggctaaatg ccaggaagtc
    99781 ctcttcgccc tgacgctgta agcgcagcgt ctgcgccagt tcactctctt cgctaaacaa
    99841 gccgtaagct ttatttttgg cgctatagac ccgatgcagt tctgccacca tctcaacgac
    99901 ggtttctgtc ggttccagca atgaatcgcg caacaccagc tcaaggttct gctcatcacg
    99961 cttgataagc tggtgcagag caatctggtt gatatccaga ctcatgataa actctccttt
   100021 aagaccgggc ggtattcaac caccgccggt taagcgacgc aataaaagat ggcggtcatt
   100081 ttacagaagg cagcgtaacg agaatgattt aacgcataaa aaagcagaaa aaaaaccgtt
   100141 gctacggtaa tatgttgccc tttcatcaac taactgattt cgatttatgc cacaaatttc
   100201 ccgctacagt gatgaacagg ttgaacaatt gctcgcagag ctgctcaacg tactggaaaa
   100261 acataaggct ccgaccgacc tttccctgat ggtgttaggc aacatggtca ctaaccttat
   100321 caacaccagc attgccccgg cccaacgcca ggcaattgcc aactcttttg cccgcgcctt
   100381 acagtcctct atcaacgaag acaaagcgca ctaagggaaa cagataacag gttatggtaa
   100441 ctcatcgtca gcgctaccgt gaaaaagtct cccagatggt cagttggggg cactggtttg
   100501 cactgttcaa tattctgctt tcgctcgtca ttggcagccg ttacctgttt atcgccgact
   100561 ggccgacaac gcttgctggt cgcatttatt cctacgtaag cattatcggc catttcagct
   100621 tcctggtgtt cgccacctac ttgctgatcc tcttcccgct gacctttatc gtcggctccc
   100681 agaggctgat gaggtttttg tccgtcattc tggcaacggc gggaatgacg ctattactga
   100741 tcgatagcga agtctttact cgtttccatc tccatcttaa tcccatcgtc tggcaactgg
   100801 ttatcaaccc agacgaaaat gagatggcgc gcgactggca gctgatgttc atcagcgtgc
   100861 cggttatttt attgcttgaa ctggtgtttg cgacgtggag ctggcaaaag ctgcgcagcc
   100921 tgacgcgtcg tcgacgcttc gcgcgcccgc tggccgcatt cttatttatc gcctttatcg
   100981 cctcgcatgt ggtgtatatc tgggccgatg ccaacttcta tcgcccgatc accatgcagc
   101041 gcgctaacct gccgctttcg tacccgatga cggcgcgacg ttttcttgag aagcatggtc
   101101 tgcttgatgc gcaggagtat caacgccgtc ttattgagca aggtaatcca gacgccgttt
   101161 ccgttcagta tccgttaagc gaactgcgct atcgcgatat gggcaccggg cagaatgtgc
   101221 tgttgattac tgtcgatggc ctgaactact cacgcttcga gaagcagatg cctgcgctgg
   101281 caggttttgc tgagcaaaat atttcgttca cgcgccatat gagctccggc aacactacag
   101341 acaacggcat ctttggcctg ttctatggca tctcgccgag ctatatggac ggcattctgt
   101401 cgacccgtac gcctgcggca ttaattactg cgcttaatca gcaaggctat cagctggggt
   101461 tattctcatc agatggcttt accagcccgc tgtatcgcca ggcattgttg tcagatttct
   101521 cgatgccgag cgtacgcacc caatccgacg agcagaccgc cacgcagtgg atcaactggc
   101581 tgggacgcta cgcacaagaa gataaccgct ggttctcgtg ggtttctttc aatggtacta
   101641 acattgacga cagcaatcag caggcatttg cacggaaata tagccgggcg gcaggcaatg
   101701 tcgatgacca gatcaaccgc gtgctcaatg cactgcgtga ttctggcaaa ctggacaata
   101761 cggtggtgat tatcactgcc ggtcggggta ttccactgag cgaagaggaa gaaacctttg
   101821 actggtccca cggtcatctg caggtgccat tagtgattca ctggccaggc acgccggcgc
   101881 agcgtattaa tgctctgact gatcataccg atctgatgac gacgctgatg caacgcctgc
   101941 tacatgtcag cacacctgcc agcgaatatt cgcaaggtca ggatttgttc aaccctcaac
   102001 gccgtcatta ctgggttacc gcagcggata acgatacgct ggcaattacc accccgaaaa
   102061 agacgctggt actgaacaat aacggtaaat accgcactta caacttacgt ggtgaaagag
   102121 tgaaagatga aaaaccacag ttaagtttgt tattgcaagt gctgacagac gagaagcgtt
   102181 ttatcgctaa ctgattaatt ataaatcagt tagcgaaata tcttacttgc aatcggtgtg
   102241 gaaaacggta gtattagcag ccacgagtcg gcacgtagcg cagcctggta gcgcaccgtc
   102301 atggggtgtc gggggtcgga ggttcaaatc ctctcgtgcc gaccaaaaat cccaagaaaa
   102361 aaccaaccct tacggttggt ttttttatat ctgcaattaa ttcgataaac agaccgtgac
   102421 acatcacagc ctgtttattt tctgttatca gaacgtccag accacacccg cctgagtatt
   102481 ccacggcgat tctacccctg cgccattgcc atagctgact gacaaatgac cgcttaacgt
   102541 cggggtaaac gatgacctta taccagcctg ataaacgcca ctggttccag ccacatcatt
   102601 attaaatttg ccatcgtcat taactttcac ctggttagaa tcggcgtatt cctgacgcac
   102661 ggccgctttc agccagggtt ccagcgtcgt accgttttgc aggtccatgt gatagcttac
   102721 cgccgttccc gcttcagcgc gtaatatccg ggtatttccc acatccgcgc gcatgccgtt
   102781 tgataacgtg tagtcctgac catctgtggt aaagccggta aaggccagat agggtctaac
   102841 actccacaat ccgtcaaccc aacggaaccc gctctcaaca tgagcacccg cgccgttact
   102901 attgtaatcg ccaaacgctg ttgccccatt actcatcttg ccatggatgg tgttggcaaa
   102961 acggtcaact ttcaccaccc catcaacata ggcaccgttc tgatgctccc aaccggcata
   103021 agcccccagg gtatagctat cgatattacc tttgccgccg cgatcaaaac caatatcaga
   103081 atgagagtaa ccaaagatca agccgcgaat tgtactgctt tcttcacggg agaaacggct
   103141 atcgataccg agcgtcaggc ccgtcaatgt ttgctcaaaa ccagctcccg catcagtggt
   103201 cacgttgttg cgggtgttaa ttgccgaact ccacatcgcc gtatcgtaac taacgccttt
   103261 tacgctacca agacgctcac gcacggtgtc cagttctgca tcaaatacca gcggttgtgc
   103321 ggccgccata ttcagcacat cagtggttga aggggtaatt tgcgcgcgat tctctgccag
   103381 actccagcta tggttgccat tatccagcaa ggtatattca tacgtaccga tatcaacaac
   103441 gcctccggca ttgcccaacg taaatgcagc atcaccgccg cccgttgtta ccagtgtaag
   103501 actatctcct gctgccgggc tggcaccggt gtccgtcacg aatattttga aatcacctgt
   103561 tgcctgaccg gtgacgttga gctgatcgct ctgatgatta gccatatcgg tacgcatata
   103621 aaaattgccg tttcccgaca gggtattggt ggtcaacgta atgaaatttt ccgcacttgc
   103681 cgtcactgat gaacgagtca ctggctcatc catcataatc acatttgcgt cagacatcgc
   103741 aaggttggct aagttgagga gggtgttttg gttcgtcgta gtgatatcgg caatgatggt
   103801 ccatacgcta ttttcgagcg aaacgtccgc tttgctggta tccacggcac catgcgttct
   103861 aaaagaagcg ccctcagaaa ccactacctt acccaccaac attgcgggtg cataatttat
   103921 ttgcggcttc atgacctcaa taatgccatc attccctctg actgaaacgt taaccattgt
   103981 gccagcccag acgttagcgc ggccattgtt gataaccata ttttcagaga tggcttttga
   104041 ggaatacgtg atacttccat tctgatatga tcgaccgaga tcatataccg catttgcctg
   104101 cactttcgta ccagtatcct ttcccagcga ttgcatagtg gcatgcttat caaggatagt
   104161 atcaatggcc tgcgtgtcct ccataacaat gagcccggaa ccatcatcca gttcataatt
   104221 ttttgacaca ccatctttta tactaaattg gcctttactg tttggaccac tcacttccag
   104281 cgcattcgtt gagacaatta attttccgcc tgctttttta tcaacgccag ttacagtccc
   104341 gccatccata acctccagta agccgccact atctacagtg gtattataag cgaagtcatt
   104401 ttcttcaact cgcaaacttc cgccgttttc caacaacata ttattagcaa taccattctt
   104461 gatatcgaac tgaccgagac ggtttgttcc gaatacctcc atggcccgcg tggttgtttt
   104521 aatagcaccg cctgctttct gatctaccgc aaatgcaaat cccccctctt taacctccag
   104581 tagtcctccc ttttcgatag cgacaaagac ggccccccca tctttttcaa cgattaagcg
   104641 cccgccttta atcgtggtat cattcgctgc gccataaacc tgtagcacac cattattgag
   104701 cgtagtctgg tttgccgcgc cacccatttg aacaatttga taaccgtcat ttaatactga
   104761 gtattctgcg gcgccattca tttcaatgta ctgataccct ccgttaattt cagtattatt
   104821 acttacacca aattctttta tatgctgttc tccgccagga ttgattttcg tattattggc
   104881 ggtcccataa accagctgga tatctttttc caggacaaca ccatcaactg tctcaccata
   104941 ctcaacagtt gatgccaatg ctatgggtga atataacgct gtagccacca gaacagatat
   105001 tgcggaacga caaagagaaa cagaaccaga ttgatgcatt gagctttcat cctatgaaat
   105061 taattgctgt taaaagcatt gggtacagaa aatacccata gctccatacc cggagtcagt
   105121 ttttaaaaac tgtttaaaga aatgcacaag tattgtgatt gattttttag ttgtttttct
   105181 tgatgagaag ctgatgcaaa attccgtctt tataatgaaa atgatgccaa agcgaacgac
   105241 aaggttgtag ttttcactac atgtccatac ataaaatggg gtaacattca cgcgcctggt
   105301 agcgttacca acgctacgct caaacataat gattctaata aaacctcagg agactactat
   105361 gcctgaagca acgccttttc aggtgatgat tgtggatgat catccactta tgcgacgcgg
   105421 tgttcgtcag ttactggagc ttgatcctgg ctttgaagtg gtcgccgaag cgggcgacgg
   105481 cgcgagcgct atcgatctgg cgaatagact ggatatcgac gtgatcttgc tggatctcaa
   105541 tatgaaaggt atgagtggcc tggatactct caatgccttg cgcagggatg gcgttaccgc
   105601 gcaaattatt atcctgaccg tatccgatgc ctccagcgat gtctttgcgc tgatagacgc
   105661 aggcgcagac ggttatctgt tgaaagacag cgacccggaa gtattgctgg aagcgattcg
   105721 tgccggagcg aaaggcagca aagtctttag cgaacgcgtc aatcagtact tacgtgaacg
   105781 tgaaatgttt ggcgcggaag aagatccctt cagcgtgctg acggagcgcg agctggatgt
   105841 tctgcacgag ctggcacagg ggctgtcaaa taaacagatt gcctcggtgt tgaatatttc
   105901 cgagcagaca gtaaaagtac atattcgcaa tctgctgcgt aaactcaatg tccgctcacg
   105961 cgtggcggcc accattctgt tcctgcaaca acgcggggca caataaaaat agcccgatgg
   106021 atttatcatc gggctgagat ttatgacaaa cgcaaaactg cctgatgcgc tacgcttatc
   106081 aggcctacgt ggatcgatca atttattgaa tttacacaat tttgtaggcc ggataaggcg
   106141 ttcacgccgc atccggcata aactaagcgc actttgtcaa cagtctagcc cgatggcatc
   106201 accatcgggc ctctttttat ttactctcct gcggcgacaa atgttgcatc gcctgcgcga
   106261 tactacgttc aatcaccgca cggcgagtat cgttggcagg taagagtttc aacatcatct
   106321 cccacgcggc aacggcttcg ccaaatcgct gctgctcaaa ggcattaaac gcatacatgc
   106381 ttagcacacg gatattgcta tggtccgttc tcaccagctg acgtagcagt tcaccgccga
   106441 ggcggttgtc gttgggatca gatgaacgag tcaacgcttc agcgtatccc agtgcagcat
   106501 cactgttttt cggatcgagg cgatacgcag tggcgtatgc atcggtggcg atactggcgt
   106561 tacccagcgc catgccaacg cggcccaaca taatccagcc ttctatatct cccggatttt
   106621 tttgcagttg agtacgcatc cccagcgcaa gacgcgacat ctcttcttcg ttgagcggat
   106681 cggctttcgg atccagcgcc ctgtccagca acgccggagc ctgtgccgtg gcctgctgcc
   106741 agattttcac ctgctgataa ttgccagtct ggtagtagct gacgccagcc acaattaacg
   106801 ccaccacaat acccggcaga taaacaacat atccggcacg cttaccttcc ggcacgcttt
   106861 gttcaggaaa cgcttccggc accacgcgta cccgacgccg cgagcgggcg tatatgaccc
   106921 aaccgccaat gccaatagcc actactggca gcacccacag cagcacggtc agcggcgtta
   106981 acggcggatc gtaagtgacg aagttgccgt aacgcgccac catataatcg acaatctctt
   107041 ttttactttt accttcctgc atcagttcat acactttctg acgcaggtcg gtggcaatca
   107101 tcgagttgga atcggcaatg ctgttgttct ggcatttcgg gcagcgcagt tcttcagtga
   107161 gctgacggaa ctgttgttcc tgtgcttcat ctttaaactg caacacatcg atggtcgcca
   107221 gcgctgagcc ggagatcatc agcatcagca cgcccaataa aaacctcatt gtgcggcctc
   107281 cttactgtat ttctcccaca gcggcttgat ctcttcttcc cagacgcgag gattcagatc
   107341 gcccgcatgg cgatagcgaa tgatgccgtt gccgtcaata agaaacgttt ctggcgcgcc
   107401 atagacaccg agatccagcc ctaacatgcc atcgccatca aacaggctta gcgcgtaagg
   107461 attgcccagc tctttcagcc agctgattgc cttctggcga tcgtctttat agttcatgcc
   107521 gaccacgcgg atgccctgcg cagaaagctg attcagatat tgatgttccg cacggcaggt
   107581 cggacaccag gtcgcccaga cgttaagcag tactggtttg ccctgagtca gcacatccgc
   107641 ctgataaaac tgccccggat tgtccagtga ttcgagacga aacttcggca caggcttgcc
   107701 aatgagcgcc gattccagat tggtcggatc atccccttcg gcattacgcg ccagctgcca
   107761 cagcagcgcc gcggcaatcg ccaggaagat aatcaacgga attaacaata ctttgcgctt
   107821 catacggcct ccggcgcagt tttttgcgga ctcacgcgct tacgatagcg aggatcaaac
   107881 agacacagca gtccgcccaa cgccatcatc agcccgcccg cccaaatcca gcgaacaaat
   107941 ggtttgtagt aaagacgcac ggcccacgcg ccgttttcca gctcttcacc gagggccgcg
   108001 tacaggtcac gcgtgatgcc gccgtcaatt gccgcttcgg tcatcatcga cccggcagtg
   108061 ttgtaataac gtttttccgc atacagcacc gtttccggct tgccatcgcg cgttacgccg
   108121 atagtcgcca caccgccacg ccagttcggg ccagtcacct ctttgacatc acggaaggtg
   108181 aagcgatatt catgaatatc gacgctatcg ccggacttca tgcgcacatc acgctcaacg
   108241 ctatagttct ggctaaaggc aatgccaaca attgtcactg ccagcccaag gtgagccgcc
   108301 accatccccc aataactgaa ggtggttttc gtgccgcgtg aaatacgtag cgcagcttcc
   108361 gcaattgcca gcaccgcaat ccagcaggcc attgccaggc cgagcaccgt catcgccaca
   108421 actttgcttt cgaacagcca cggcaacagc agcgacagca ccagcgtaga gatgaaggcg
   108481 ataatcaata aattgcggat cttacgcggg cgatcccgcc cccagcgcac cagaggaccg
   108541 acaccaagca gtagcgcaaa cggcaccatc agccaggtaa acatggtgtt gaagaacggt
   108601 tcgccaatcg aaatactgcc cagtcccagt tgcttatgca ccaacggcag cagcgtcccc
   108661 agcaacacca ccagcatcgc agcgaccagc aaaacattgt tcgctaacag caaagattcc
   108721 cgcgaccaca gcgcattgtt tacgcgtgag cgaactttgt gtccacgcgc ggcaaacagc
   108781 agcagcgaac cgccaatcac cagcaccata aaggcgagga taaacatacc gcgcgccgga
   108841 tcagacgcaa acgcgtgtac cgataccagc acgccggaac gcacgaggaa agtccccaac
   108901 agacacaacg agaaggcact gattgccagc agtaatgtcc acgctttgaa gctggcgcgt
   108961 tgttcagtga ccgccagtga gtgcatcagc gcagtcccca ccagccacgg cataaacgag
   109021 gcgttttcta ccggatccca gaaccaccag ccaccccagc cgagttcgta ataggcccat
   109081 gcggaaccga gcacgatgcc gagcgtcagg aagatccacg ctgccagcgt ccacggacga
   109141 gtaaaacgcg cataagtgct gtccagacgc ccgctcagca aagaagcaat ggcaaaagca
   109201 aacgccaccg agaaacccac gtaccccata taaagcagag gcggatggaa gatcagcccc
   109261 ggatcctgca atagcgggtt aagatcgcga ccttcaatcg ggaagttcgg caacgtgcga
   109321 gagaacgggt tagaggtaaa gagaatgaac agcaaaaagc cgacactgac catccccatt
   109381 atcgccagta cacgggccac aatatccagc ggaatacgct gactaaaaat cgccaccgca
   109441 aaggtccagc cgctcatcag cagtacccac agcagtagcg agccttcatg cgcgccccag
   109501 gtagccgcca cgcgatacca caccggaagc tgggtattgg agttgctggc aacataggtg
   109561 acggtgaagt cgttgaccac gaaagcattg accagcacca gaaatgcgcc agccacagac
   109621 ataaacagca gccaggcaaa caagcgggaa gacgccatca tgcgcgcatc tccgcgcgcc
   109681 acgccccata gcggatacac ggacagcagc agcgcaattc ccagcgccag gcacagcagt
   109741 ccgttaccaa tttctggcat catgatgctg ggtccttata aacactcgcc gggcgacggt
   109801 ggttagcttc catcgctttc tcaacttctg gcggcgtata gttttcatcg tgtttcgcca
   109861 gcacttcttt cgcgaggata tgattgcctt tttccagttc gccctgcacc acaacgccct
   109921 gcccttcacg gaacagatcc ggcaaaatgc cttcgtaaga gacatccact gagccttcag
   109981 catcgtaaat ggtgaaggtc actttcagcg aattgggatc gcgctgcaca ctacccggca
   110041 tcaccatccc gccaacgcgc agacgctgac cgacttccgg catttgctga gtttcacgct
   110101 tgccgtagag aatttccccc ggcgtataaa agagatcgat attcgagcgc agcgcatata
   110161 gcaccagacc gatagtcagc gccagccctg ccaacacggc acaggcaatc cacaagcggt
   110221 ttttacggcg aatattcatg cagcctcctg ctgttgcgca gcacgtaaac gcgcctcacg
   110281 cgcccgctgt tgcgccacgc cacgcagaat tgcgcgatgt tgtatcaccg agtgcacgac
   110341 caaaaccacc agcggaataa cggtcatcac caccgccagc cagacaaaaa aggcgtaacc
   110401 gcccattgcg aaaaattcat tccaggaagc aaatgcaggg gtcatttacg gcctcttttc
   110461 agtatcagtt cactcaccca cggacggcgt ttttccatca gcaaaatcaa attacgcatc
   110521 cgcatcagcg tcagcgtggc agacaggagc aggaagccaa aaatcgacca gcgcagcggc
   110581 gaacgcatcg ccggatcgat actttgctgc atccgcgttg atccctgatg cagggtgttc
   110641 caccactcca cggagtaatg aataatcggc agattcacca cgccaatcag caccaggata
   110701 cctgccgcac ggcccgccag acggcggtcg tcgaaggcgt gccacagggc aatcacaccc
   110761 acatacaaaa acagcagcac cagttcagaa gtcagacgtg catcccatac ccaccaggtg
   110821 ccccacatcg gttttcccca tgcagagccg gtaaccaggg caataaaggt aaacacggca
   110881 ccaatggggg ccatcgccgc caccgccagg ttggccattt tcatctgcca gacaaggcca
   110941 ataaacgctg ccactgccat tgatgcataa atgcccatcg accagatcgc cgcaggcaca
   111001 tgcaggtaga taatgcggta gctatttccc tgctgataat cagccggagc aaagccgaat
   111061 ccccagatcc agccgacggt aagcacgacc acactggcaa ttgccagcca cggtataaac
   111121 cagccacaga tttgatacag ccgtggtggg atcgccagtt gatgcagtgt tttccacata
   111181 gtttcgatac cagactcgaa caaaaatcag taatccagcg ttattgaatg ctgattcgta
   111241 acgctgccgc cgtcgcaaaa ggacttaatg tcgcggtgcc tgccagcaac gcgcctaaaa
   111301 ttgccagata cccgtcaacg ggcaaatgca tagaagccgc gtccatcgcg gcggtggcaa
   111361 agatgagtaa tgggatagtc agcggtaaca ccagtatgct gagcagcaca ccaccgcgct
   111421 taagtcccac tgtcagcgcc acgcccggtg caccgagaaa gccaagcgta ggcgttccca
   111481 gcagcagcgt cagcgccatc acttgccagc cataaacatc cattcccagt agcattgcta
   111541 ccagtggcga aaggatgagt aacggcagac cggttaccat ccagtgcgcc atcaccttcg
   111601 ccagcacaac ggcgggcaag ggtaacggca acaacatcaa ttgttcaaga ctgccgtcct
   111661 gcaaatcgtc acggaacagt cgttccagcg ccagcaagga tgaaagcagc gcagcaaccc
   111721 agataatgcc cggtgcaata cgcgccagca gttgcggctc cggaccgata ctgagcggaa
   111781 aaagggtaat tacaatcagg aagaaccaca gcgggttggc gatttcggcg ctatggcgaa
   111841 acgctacacg cagctcaaga cggaaaatgc gccagaacat catgcggccc tcgtttgcgt
   111901 cagtgaaatg cggcgaattt tactttcagc aacgttgagc ggctggtggg tagtcagaat
   111961 cacaatcccc ccctgctccg tatgctgcgc catacgctgg gtcagacgat cgacaccgtt
   112021 aacgtcaatc gcggtaaaag gctcgtcgag gatccataac gtggcacggg tcagccacag
   112081 acgcgctaaa gcgacgcggc gttgttgccc ggccgagagc tgatttacag gaatatcttc
   112141 gaatccggca agcccggcct gcgccagggc ttccagacat tgtgcggtat cgccatcgcg
   112201 atgataaaag tgcagatttt ctaacgccgt cagccgggtt ttgatccccg gctgatggcc
   112261 tatccataac aggttttgat ggtagctgtc gcgtacctga tgcaagggct gcccttgcca
   112321 gagaacctcg cctgcgtcag ggcgagacaa ccccgtcagc aaacggagaa gcgttgtctt
   112381 ccccgcgccg ttgctaccgg tgatttgtac ccactctcct gcgttcagcg taaatgacaa
   112441 gccactaaat aaggttcgtt catcccgctc acaaagtaac tctctggctt caagcatacc
   112501 cacgcaataa ccctgttaaa aacctggctc gacttcacgc atatcgggca gcttgtgcgc
   112561 tatcccttta tggcaatcaa tacaggtttg cccatctttc accgcctggt catgcatctt
   112621 cgcggcaacc gatttctggg cggttgtatc catatactcg aagttgtgac agttacggca
   112681 ctcctgcgag ttattgtcct tcatgcgccg ccactcattc tgtgccatcg tcagacgatg
   112741 agcttcaaat ttctgcggcg tgtcaataac gccaaaaatt ttaccataca gctctttact
   112801 tgctttgagc ttgcgtatca tcttcggcac aaactcgtgc ggaacgtgac aatccggaca
   112861 ggtcgcacgg acgccgctac ggttgttgta gtgcacggaa tccatgtatt cctgatacac
   112921 cgtgttgcgc atttcgtggc agctaatgca gaactcttcg gtattggctt tttccatccc
   112981 ggtgttaaag ccaccccaga agacgatgcc gccaacaaaa ccgatcaaca gcagcgtccc
   113041 cagcgccaga cggctggggg tacgccacca tttccacagg cgcttaatca gaccaggctt
   113101 acggtcagaa tttcccataa taacctctta tttcccgtaa ccttttgatg gggtaaaggt
   113161 attccccacg attggcgcgg tatcggcctg cggtacgtga cattgcagac agaaataacg
   113221 acgcggagcc acttccgcgc ccactttgcc gtcgctgtcc ataaagtgag taggactgat
   113281 acgcggcgcg ccagtggtgc gatagctttc gacaccgtgg cactgcaagc agcgattggt
   113341 attggtcgtt acctgataac cttcaacgct atgcgggatc attggcggct gattcacata
   113401 gttcagcggc atccgatcct gctcttttgg catccgaatg gccccttcct gtgtcccgga
   113461 tacttccggc gactggctaa aatcgacgcc attagccgcc caaaccgcgc cgcttaccac
   113521 cagggccagc atcgccgtcc attgacacag cgctttcttc aggtcatggc ttttcatgat
   113581 ttcgctcccg aactccatcg tgtagttatt gtaaatacat cctcagaaca gacatccacg
   113641 cagcgaccgc acgtcatgca atcgcggctg gtgacctgca ccgggctttg ttcatccagc
   113701 accggggcac gtagcacatg cggttccggg caaacatgaa aacaatccat acagcggtta
   113761 catttctgcc gatcggtggc cgcaacggta atcacacctt tgctacccag cacgccatac
   113821 agcgcgccaa ccggacaaat gtgcccgcac cagccgtgtt caacgaccag tagatcaaat
   113881 aaaaacagcg cgagaataag cagcgcgccg ctgccgaagc ccattaccag gctacgcccc
   113941 atcagagaaa cggggttgat ccattcccaa atgagcgtgc cagttaaggc tgatcccacc
   114001 agaatgacca ccaacagcac gtagcgaatg tggcgaggga tcgtcgcaga ctgattgagg
   114061 tcaaagcgcc tgcgtaacca gttcgctaag tcggtaatcg ggttcagcgg gcagacccag
   114121 ctgcaaaata atcgctttcc cgccagggcg tagagcacgg tgataatcac cgcaccggtc
   114181 aacgccaccg tggcaggcag atgaccactg gcaagacttt gcagcgtcat cagcggatcg
   114241 gtcagcggaa cggtgtcgaa taacaggcta ctgctgtagt tgccgtgcaa gatccacaca
   114301 ccaaaccatg gaccgctcaa aaacatcccc agcacgaaga actgacaaag gcgacgtaac
   114361 accagccaac ggtgactgcg ccaccagcct tttttctcca gcgcctcgcg cccggcgtca
   114421 cgtttacgat ttgccattgt tcccctccag ccagccgaag cggtaatggt gacctaactc
   114481 ccctttcgcc agtgacagcg gtaacacctt gattgccggt tgttccagca cgcatacttt
   114541 ttcgcactta ccgcaaccgg tacaggcgtc gctgtgaacc gtcggcagaa agcgggcgtg
   114601 cttaccggta cgcgtgttgc gctccagctc cagggtgatg gcctcatcaa ttttcgggca
   114661 ttcgcgataa caaacatcgc agcgcagccc ctgaaagttg agacagtttt cctggtccac
   114721 cagtaccgcc agccccatcc gcgcgtcgtc gatcgattca atctcacgat ccagcgcacc
   114781 gcttgggcac actttggcgc acggaatgtc ctcacacatt tcgcaaggaa tatcccgtgc
   114841 gacaaaatat ggcgtgcccg ccgacagacc agaggccagc gtcgccagtt ttaaggtgtc
   114901 gtaagggcaa gcctgaacac actgaccgca acgcacacag gcactggcaa aggcgttctc
   114961 gtttatggct ccgggcgggc gcaaccgcac gccagatgcg cgtgcggttt gctgttgtaa
   115021 ccccagcgcc acacccacgg cagccagccc gcctgctgtg cgaacgacat cgcgcagaaa
   115081 gcggcggcga ccattttgag gtttcgctga ccgggacata atggcttaca ccttctccag
   115141 tttgaccgcg cacttcttga agtccgtctc tttcgagagc ggatcggtcg catccagcgt
   115201 cagtttgtta accaactgtg cggcgtcgaa gaacggcatg tacaccaggc cctgtggcgg
   115261 acggttacga ccgcgcgttt caacaatcga gatcacttcg ccacggcgag aaaccacttt
   115321 cactttgtcg ccacggcgca gatcgcgcgc tttcgcatcc agcgggtgaa taaacaggac
   115381 cgcttccggg aaggcgcggt gcagttctgg tacacggcga gtcatactgc cggtgtgcca
   115441 gtgctccaga acgcgtccgg tagagagcca caggtcgtac tcttcatccg gtgcttccgc
   115501 cgccggttcg aatggcagtg cgaagatcac cgctttgcca tccggtttac cgtagaactt
   115561 atagccttcg cccgctttca cgtacgggtc gttaccttcg ctgtaacgcc actgcgtttc
   115621 tttaccgttc accaccggcc agcgcagacc gcgcgctttg tggtagtcat cgaacggtgc
   115681 cagatcgtga ccgtgaccgc gaccaaacca tgcgtactct tcgaacagcc ctttttgcag
   115741 atagaagccc agctcgcggg attcatcgtt cagctgatct tccgccagtt cggataccgg
   115801 gaatttgctc acttccggtg tggcatacag cacttcgtac agcgttttgc cacgcagttc
   115861 cggtttcttc gccagcagat cttccggcca tacttcttca gttttgaagc ggcgggagaa
   115921 ctggactaac tgccagagat ccgatttcgc ttcgcccggt gcctgtacct gttgacgcca
   115981 gaactgagta cggcgttcgg cgttaccgta agcgccctct ttctctaccc acattgcggt
   116041 cggcaggatc aagtcggcgg ccagcgcact gactgtcgga tacggatcgg agacgatgat
   116101 gaagttgcgc ggatcgcgcc agcccggcat acgctcttca ttgatgttcg gcccggcctg
   116161 catgttgttg gtacacatgg tccagtaaac attcagtttg ccgtctttca gcgcacggtc
   116221 ttgtgccacc gcgtgcagac cgattttcgc cggaatggtg ccgctcggga tattccactt
   116281 cttctcgcag atatcacggt gtttctcgtt ggtcaccacc atgtccgcag gcagacggtg
   116341 agcaaaggtg cccacttcac gcgcagtacc acacgcagaa ggttgcccgg tcagggagaa
   116401 cggaccgcaa cccggctggg aaattttgcc ggtcagcagg tgcaggttgt agaccaggtt
   116461 gttagcccac acgccacgag tatgctggtt gaagcccatc gtccagtagg agatgacttt
   116521 cttgttcgga tcggcataca gctgcgccag ttgttctaac tggtctttcg gcacgccggt
   116581 catttcggca gttttttcca gcgtatactc ggcaacgaag gctttgtaat cttcaaagct
   116641 catcggttcg gaggcgtcag aacccggatt cttcgctgct ttttccagcg gatgggtcgg
   116701 acgtaaaccg tagccgatgt ccgtcgcccc tttgcgcagg ttaacgtgct tgctgaagaa
   116761 gtcctgattt atcgcattgt tttgaatgat atagttggcg atgtagttca ggatcaccag
   116821 gtcagattgc ggcgtaaaga tgatgccgtt atccgccagc tcgaagctac gatgctggta
   116881 ggtagaaagc accgccacgg tgacgttctg gttagagaga cgacggttag tgatgcgcga
   116941 ccagaggatc gggtgcattt ccgccatgtt tgcgccccac agcacaaacg cgtcagcctg
   117001 ctcgatgtcg tcatagcagc ccatcggctc atccatacca aaggtacgca taaagccaac
   117061 tactgccgac gccatacagt gacgcgcgtt cgggtcgatg ttgttcgaac ggaagcccgc
   117121 tttaaacagc ttggacgcgg cataaccttc ccagatagtc cactgaccag aaccgaacat
   117181 accgatcgat tccggccctt tttctttcag ggcggttttg aatttctctt ccatcacatc
   117241 gaaggcctga tcccaggtga ttggggtaaa ttcgccttct ttgtcatatt taccgttttt
   117301 catacgcagc agcggctgcg tcaaacggtc tttaccgtac atgattttgg gcaggaaata
   117361 gcccttaatg cagttcaggc cacggttaac cggtgcgtcc gggtcgccct gacaggccac
   117421 cacacgtccc tgctgcgttc cgaccagaac gccgcaacca gtaccgcaga aacggcacgg
   117481 cgctttatcc catttgatgg cttcctgctg accaacaacg gcgcgggcaa cgcccggcac
   117541 gctgagaccg gcagccgccg cagcggccgc aacggcgtta gctttcataa agctacgacg
   117601 actgagtttc atggtgtttc ctcaccttgc tcttcctgct ggtgataaac cagcgacacc
   117661 gccagcacgc cctctacgtt gcgtactgac tcaatggttt ggatcagcgt ttcgctgtct
   117721 tctgcttcca ccaccacaat caactgaccg ctcggcgcgt cgctgacagc aacttcacag
   117781 ccgggaaagg cgttcagttg ggtgctgatg tctgaaattc gttcgctttt ggcctgcacg
   117841 accaggctgc aaacttgcca gttagtgtgc atggagatac tccgcagtta tggctgatac
   117901 cggacagctg gctgcacatg ctccacatcc gttgcaaagt tggctattaa gttgcggctg
   117961 gtagatcccg gagagcgtcg ggcgaaagat aattgccatt ggctcacagc tatcctgaca
   118021 gcggcggcat tcaactgact gatacgccag gcacgcgtcc ccgatggtga actgcaaatc
   118081 ccaggccctg gtgtggcgcg gagaaaatag cgattcgggg caagcctgcg cgcaggcgta
   118141 acagaagctg cactcgttat ttttgaaatt aacgctcgga tagccgcccg cgccgcgttg
   118201 cagaatgttg ttttcacaag cattaataca ggcgtcgcag cgggtgcaat gggtcagaaa
   118261 atgagattca tcaccggacc agggcggacg gataccgtta ctggctttgc gccagcgacc
   118321 agtgagtatg ccccgacggg atgcatcaat cttcacattg accttccatc attaacgcgc
   118381 tctgaaaatt gagagcgacc aaataaaccg cataattaat aagccatttt tatagccgct
   118441 aaga
//
LOCUS       DBBGLQ010000004        97440 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.004, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000004 DBBGLQ010000000
VERSION     DBBGLQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 97440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.004"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..1720
                     /gene="adeD"
                     /locus_tag="ACIYSB_000388"
     CDS             <1..1720
                     /gene="adeD"
                     /locus_tag="ACIYSB_000388"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGC5427509.1"
                     /translation="YQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIA
                     GVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICD
                     PHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRD
                     HPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENC
                     HESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIA
                     HEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDAR
                     KVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRY
                     RVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLNEGA
                     LAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMST
                     DTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFT
                     TLEVTE"
     gene            complement(1766..3157)
                     /gene="uhpT"
                     /locus_tag="ACIYSB_000389"
     CDS             complement(1766..3157)
                     /gene="uhpT"
                     /locus_tag="ACIYSB_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGC5427510.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3295..4614)
                     /gene="uhpC"
                     /locus_tag="ACIYSB_000390"
     CDS             complement(3295..4614)
                     /gene="uhpC"
                     /locus_tag="ACIYSB_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGC5427511.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4624..6126)
                     /gene="uhpB"
                     /locus_tag="ACIYSB_000391"
     CDS             complement(4624..6126)
                     /gene="uhpB"
                     /locus_tag="ACIYSB_000391"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGC5427512.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6126..6716)
                     /gene="uhpA"
                     /locus_tag="ACIYSB_000392"
     CDS             complement(6126..6716)
                     /gene="uhpA"
                     /locus_tag="ACIYSB_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGC5427513.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6792..7082)
                     /gene="ilvN"
                     /locus_tag="ACIYSB_000393"
     CDS             complement(6792..7082)
                     /gene="ilvN"
                     /locus_tag="ACIYSB_000393"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGC5427514.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7086..8774)
                     /gene="ilvB"
                     /locus_tag="ACIYSB_000394"
     CDS             complement(7086..8774)
                     /gene="ilvB"
                     /locus_tag="ACIYSB_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGC5427515.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQASLQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8880..8978)
                     /gene="ivbL"
                     /locus_tag="ACIYSB_000395"
     CDS             complement(8880..8978)
                     /gene="ivbL"
                     /locus_tag="ACIYSB_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGC5427516.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9543..9632
                     /gene="tisB"
                     /locus_tag="ACIYSB_000396"
     CDS             9543..9632
                     /gene="tisB"
                     /locus_tag="ACIYSB_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGC5427517.1"
                     /translation="MNLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9756..9830)
                     /gene="ysdE"
                     /locus_tag="ACIYSB_000397"
     CDS             complement(9756..9830)
                     /gene="ysdE"
                     /locus_tag="ACIYSB_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGC5427518.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9912..11096
                     /gene="emrD"
                     /locus_tag="ACIYSB_000398"
     CDS             9912..11096
                     /gene="emrD"
                     /locus_tag="ACIYSB_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGC5427519.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11104..11601)
                     /gene="yidF"
                     /locus_tag="ACIYSB_000399"
     CDS             complement(11104..11601)
                     /gene="yidF"
                     /locus_tag="ACIYSB_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGC5427520.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11598..11960)
                     /gene="yidG"
                     /locus_tag="ACIYSB_000400"
     CDS             complement(11598..11960)
                     /gene="yidG"
                     /locus_tag="ACIYSB_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGC5427521.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11950..12297)
                     /gene="yidH"
                     /locus_tag="ACIYSB_000401"
     CDS             complement(11950..12297)
                     /gene="yidH"
                     /locus_tag="ACIYSB_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGC5427522.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12405..12854
                     /gene="yidI"
                     /locus_tag="ACIYSB_000402"
     CDS             12405..12854
                     /gene="yidI"
                     /locus_tag="ACIYSB_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGC5427523.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILNQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12901..14394)
                     /gene="yidJ"
                     /locus_tag="ACIYSB_000403"
     CDS             complement(12901..14394)
                     /gene="yidJ"
                     /locus_tag="ACIYSB_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGC5427524.1"
                     /translation="MKRPNFLFVMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14391..16106)
                     /gene="yidK"
                     /locus_tag="ACIYSB_000404"
     CDS             complement(14391..16106)
                     /gene="yidK"
                     /locus_tag="ACIYSB_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGC5427525.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     GKDSL"
     gene            16273..17166
                     /gene="yidL"
                     /locus_tag="ACIYSB_000405"
     CDS             16273..17166
                     /gene="yidL"
                     /locus_tag="ACIYSB_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGC5427526.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17163..17977)
                     /locus_tag="ACIYSB_000406"
                     /pseudo
     CDS             complement(17163..17977)
                     /locus_tag="ACIYSB_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17977..19593)
                     /locus_tag="ACIYSB_000407"
     CDS             complement(17977..19593)
                     /locus_tag="ACIYSB_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGC5427527.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDRLINSHQSAENVAITEAV"
     gene            19889..20605
                     /gene="yidP"
                     /locus_tag="ACIYSB_000408"
     CDS             19889..20605
                     /gene="yidP"
                     /locus_tag="ACIYSB_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGC5427528.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20602..22263)
                     /gene="yidE"
                     /locus_tag="ACIYSB_000409"
     CDS             complement(20602..22263)
                     /gene="yidE"
                     /locus_tag="ACIYSB_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGC5427529.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22459..22887)
                     /gene="ibpB"
                     /locus_tag="ACIYSB_000410"
     CDS             complement(22459..22887)
                     /gene="ibpB"
                     /locus_tag="ACIYSB_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGC5427530.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(22999..23412)
                     /gene="ibpA"
                     /locus_tag="ACIYSB_000411"
     CDS             complement(22999..23412)
                     /gene="ibpA"
                     /locus_tag="ACIYSB_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGC5427531.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23406..23478)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23718..24050
                     /gene="yidQ"
                     /locus_tag="ACIYSB_000412"
     CDS             23718..24050
                     /gene="yidQ"
                     /locus_tag="ACIYSB_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGC5427532.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24052..25266)
                     /gene="yidR"
                     /locus_tag="ACIYSB_000413"
     CDS             complement(24052..25266)
                     /gene="yidR"
                     /locus_tag="ACIYSB_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGC5427533.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIVE
                     GGKMSNLDAMDITAPYTPGVLRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTEHHANSPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25367..26431
                     /gene="cbrA"
                     /locus_tag="ACIYSB_000414"
     CDS             25367..26431
                     /gene="cbrA"
                     /locus_tag="ACIYSB_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGC5427534.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKTVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSTDILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(26428..27765)
                     /gene="dgoT"
                     /locus_tag="ACIYSB_000415"
     CDS             complement(26428..27765)
                     /gene="dgoT"
                     /locus_tag="ACIYSB_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5427535.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27840..28988)
                     /gene="dgoD"
                     /locus_tag="ACIYSB_000416"
     CDS             complement(27840..28988)
                     /gene="dgoD"
                     /locus_tag="ACIYSB_000416"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HGC5427536.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(28985..29602)
                     /gene="dgoA"
                     /locus_tag="ACIYSB_000417"
     CDS             complement(28985..29602)
                     /gene="dgoA"
                     /locus_tag="ACIYSB_000417"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HGC5427537.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29586..30464)
                     /gene="dgoK"
                     /locus_tag="ACIYSB_000418"
     CDS             complement(29586..30464)
                     /gene="dgoK"
                     /locus_tag="ACIYSB_000418"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HGC5427538.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREEKTPVVMAGMVGSNVGWKVAPYLSVPACFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30461..31150)
                     /gene="dgoR"
                     /locus_tag="ACIYSB_000419"
     CDS             complement(30461..31150)
                     /gene="dgoR"
                     /locus_tag="ACIYSB_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HGC5427539.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31428..32084
                     /gene="yidX"
                     /locus_tag="ACIYSB_000420"
     CDS             31428..32084
                     /gene="yidX"
                     /locus_tag="ACIYSB_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGC5427540.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32130..32942)
                     /gene="yidA"
                     /locus_tag="ACIYSB_000421"
     CDS             complement(32130..32942)
                     /gene="yidA"
                     /locus_tag="ACIYSB_000421"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGC5427541.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33057..33455)
                     /gene="yidB"
                     /locus_tag="ACIYSB_000422"
     CDS             complement(33057..33455)
                     /gene="yidB"
                     /locus_tag="ACIYSB_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGC5427542.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33695..36109)
                     /gene="gyrB"
                     /locus_tag="ACIYSB_000423"
     CDS             complement(33695..36109)
                     /gene="gyrB"
                     /locus_tag="ACIYSB_000423"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGC5427543.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36138..37211)
                     /gene="recF"
                     /locus_tag="ACIYSB_000424"
     CDS             complement(36138..37211)
                     /gene="recF"
                     /locus_tag="ACIYSB_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGC5427544.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37211..38311)
                     /gene="dnaN"
                     /locus_tag="ACIYSB_000425"
     CDS             complement(37211..38311)
                     /gene="dnaN"
                     /locus_tag="ACIYSB_000425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGC5427545.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38316..39719)
                     /gene="dnaA"
                     /locus_tag="ACIYSB_000426"
     CDS             complement(38316..39719)
                     /gene="dnaA"
                     /locus_tag="ACIYSB_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGC5427546.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40016..40096
                     /locus_tag="ACIYSB_000427"
     CDS             40016..40096
                     /locus_tag="ACIYSB_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427547.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40326..40466
                     /gene="rpmH"
                     /locus_tag="ACIYSB_000428"
     CDS             40326..40466
                     /gene="rpmH"
                     /locus_tag="ACIYSB_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGC5427548.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40483..40842
                     /gene="rnpA"
                     /locus_tag="ACIYSB_000429"
     CDS             40483..40842
                     /gene="rnpA"
                     /locus_tag="ACIYSB_000429"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGC5427549.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40806..41063
                     /gene="yidD"
                     /locus_tag="ACIYSB_000430"
     CDS             40806..41063
                     /gene="yidD"
                     /locus_tag="ACIYSB_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGC5427550.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41066..42712
                     /gene="yidC"
                     /locus_tag="ACIYSB_000431"
     CDS             41066..42712
                     /gene="yidC"
                     /locus_tag="ACIYSB_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGC5427551.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYSVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42818..44182
                     /gene="mnmE"
                     /locus_tag="ACIYSB_000432"
     CDS             42818..44182
                     /gene="mnmE"
                     /locus_tag="ACIYSB_000432"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGC5427552.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            44425..44499
                     /gene="tnaC"
                     /locus_tag="ACIYSB_000433"
     CDS             44425..44499
                     /gene="tnaC"
                     /locus_tag="ACIYSB_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGC5427553.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            44720..46135
                     /gene="tnaA"
                     /locus_tag="ACIYSB_000434"
     CDS             44720..46135
                     /gene="tnaA"
                     /locus_tag="ACIYSB_000434"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGC5427554.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            46226..47473
                     /gene="tnaB"
                     /locus_tag="ACIYSB_000435"
     CDS             46226..47473
                     /gene="tnaB"
                     /locus_tag="ACIYSB_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGC5427555.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            47605..48780
                     /gene="mdtL"
                     /locus_tag="ACIYSB_000436"
     CDS             47605..48780
                     /gene="mdtL"
                     /locus_tag="ACIYSB_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGC5427556.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            48755..49714
                     /gene="yidZ"
                     /locus_tag="ACIYSB_000437"
     CDS             48755..49714
                     /gene="yidZ"
                     /locus_tag="ACIYSB_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGC5427557.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            49871..50620
                     /gene="yieE"
                     /locus_tag="ACIYSB_000438"
     CDS             49871..50620
                     /gene="yieE"
                     /locus_tag="ACIYSB_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGC5427558.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            50642..51208
                     /gene="yieF"
                     /locus_tag="ACIYSB_000439"
                     /gene_synonym="chrR"
     CDS             50642..51208
                     /gene="yieF"
                     /locus_tag="ACIYSB_000439"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGC5427559.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(51262..52599)
                     /gene="adeP"
                     /locus_tag="ACIYSB_000440"
     CDS             complement(51262..52599)
                     /gene="adeP"
                     /locus_tag="ACIYSB_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGC5427560.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            52766..53431
                     /gene="yieH"
                     /locus_tag="ACIYSB_000441"
     CDS             52766..53431
                     /gene="yieH"
                     /locus_tag="ACIYSB_000441"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGC5427561.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            53498..53971
                     /gene="cbrB"
                     /locus_tag="ACIYSB_000442"
     CDS             53498..53971
                     /gene="cbrB"
                     /locus_tag="ACIYSB_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HGC5427562.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            54020..54607
                     /gene="cbrC"
                     /locus_tag="ACIYSB_000443"
     CDS             54020..54607
                     /gene="cbrC"
                     /locus_tag="ACIYSB_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HGC5427563.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(54669..55391)
                     /gene="yieK"
                     /locus_tag="ACIYSB_000444"
     CDS             complement(54669..55391)
                     /gene="yieK"
                     /locus_tag="ACIYSB_000444"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGC5427564.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(55406..56575)
                     /gene="yieL"
                     /locus_tag="ACIYSB_000445"
     CDS             complement(55406..56575)
                     /gene="yieL"
                     /locus_tag="ACIYSB_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGC5427565.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(56602..58218)
                     /gene="bglH"
                     /locus_tag="ACIYSB_000446"
     CDS             complement(56602..58218)
                     /gene="bglH"
                     /locus_tag="ACIYSB_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HGC5427566.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(58304..59698)
                     /gene="bglB"
                     /locus_tag="ACIYSB_000447"
     CDS             complement(58304..59698)
                     /gene="bglB"
                     /locus_tag="ACIYSB_000447"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HGC5427567.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPRGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(59717..61594)
                     /gene="bglF"
                     /locus_tag="ACIYSB_000448"
     CDS             complement(59717..61594)
                     /gene="bglF"
                     /locus_tag="ACIYSB_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HGC5427568.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(61727..62563)
                     /gene="bglG"
                     /locus_tag="ACIYSB_000449"
     CDS             complement(61727..62563)
                     /gene="bglG"
                     /locus_tag="ACIYSB_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HGC5427569.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPPEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALAIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(62849..63574)
                     /gene="phoU"
                     /locus_tag="ACIYSB_000450"
     CDS             complement(62849..63574)
                     /gene="phoU"
                     /locus_tag="ACIYSB_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGC5427570.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(63589..64362)
                     /gene="pstB"
                     /locus_tag="ACIYSB_000451"
     CDS             complement(63589..64362)
                     /gene="pstB"
                     /locus_tag="ACIYSB_000451"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGC5427571.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(64545..65435)
                     /gene="pstA"
                     /locus_tag="ACIYSB_000452"
     CDS             complement(64545..65435)
                     /gene="pstA"
                     /locus_tag="ACIYSB_000452"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGC5427572.1"
                     /translation="MAMVEMQTNAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(65435..66394)
                     /gene="pstC"
                     /locus_tag="ACIYSB_000453"
     CDS             complement(65435..66394)
                     /gene="pstC"
                     /locus_tag="ACIYSB_000453"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGC5427573.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(66481..67521)
                     /gene="pstS"
                     /locus_tag="ACIYSB_000454"
     CDS             complement(66481..67521)
                     /gene="pstS"
                     /locus_tag="ACIYSB_000454"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGC5427574.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(67835..69664)
                     /gene="glmS"
                     /locus_tag="ACIYSB_000455"
     CDS             complement(67835..69664)
                     /gene="glmS"
                     /locus_tag="ACIYSB_000455"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGC5427575.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(69826..71196)
                     /gene="glmU"
                     /locus_tag="ACIYSB_000456"
     CDS             complement(69826..71196)
                     /gene="glmU"
                     /locus_tag="ACIYSB_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGC5427576.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(71549..71968)
                     /gene="atpC"
                     /locus_tag="ACIYSB_000457"
     CDS             complement(71549..71968)
                     /gene="atpC"
                     /locus_tag="ACIYSB_000457"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGC5427577.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(71989..73371)
                     /gene="atpD"
                     /locus_tag="ACIYSB_000458"
     CDS             complement(71989..73371)
                     /gene="atpD"
                     /locus_tag="ACIYSB_000458"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGC5427578.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(73398..74261)
                     /gene="atpG"
                     /locus_tag="ACIYSB_000459"
     CDS             complement(73398..74261)
                     /gene="atpG"
                     /locus_tag="ACIYSB_000459"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGC5427579.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(74312..75853)
                     /gene="atpA"
                     /locus_tag="ACIYSB_000460"
     CDS             complement(74312..75853)
                     /gene="atpA"
                     /locus_tag="ACIYSB_000460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGC5427580.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(75866..76399)
                     /gene="atpH"
                     /locus_tag="ACIYSB_000461"
     CDS             complement(75866..76399)
                     /gene="atpH"
                     /locus_tag="ACIYSB_000461"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGC5427581.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(76414..76884)
                     /gene="atpF"
                     /locus_tag="ACIYSB_000462"
     CDS             complement(76414..76884)
                     /gene="atpF"
                     /locus_tag="ACIYSB_000462"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGC5427582.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(76946..77185)
                     /gene="atpE"
                     /locus_tag="ACIYSB_000463"
     CDS             complement(76946..77185)
                     /gene="atpE"
                     /locus_tag="ACIYSB_000463"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGC5427583.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(77232..78047)
                     /gene="atpB"
                     /locus_tag="ACIYSB_000464"
     CDS             complement(77232..78047)
                     /gene="atpB"
                     /locus_tag="ACIYSB_000464"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGC5427584.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(78056..78436)
                     /gene="atpI"
                     /locus_tag="ACIYSB_000465"
     CDS             complement(78056..78436)
                     /gene="atpI"
                     /locus_tag="ACIYSB_000465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGC5427585.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(79053..79676)
                     /gene="rsmG"
                     /locus_tag="ACIYSB_000466"
     CDS             complement(79053..79676)
                     /gene="rsmG"
                     /locus_tag="ACIYSB_000466"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGC5427586.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(79740..81629)
                     /gene="mnmG"
                     /locus_tag="ACIYSB_000467"
     CDS             complement(79740..81629)
                     /gene="mnmG"
                     /locus_tag="ACIYSB_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGC5427587.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQEVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(82008..82451)
                     /gene="mioC"
                     /locus_tag="ACIYSB_000468"
     CDS             complement(82008..82451)
                     /gene="mioC"
                     /locus_tag="ACIYSB_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGC5427588.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     PASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWVNLLK"
     gene            complement(82541..82999)
                     /gene="asnC"
                     /locus_tag="ACIYSB_000469"
     CDS             complement(82541..82999)
                     /gene="asnC"
                     /locus_tag="ACIYSB_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGC5427589.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            83151..84143
                     /gene="asnA"
                     /locus_tag="ACIYSB_000470"
     CDS             83151..84143
                     /gene="asnA"
                     /locus_tag="ACIYSB_000470"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGC5427590.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(84148..85599)
                     /gene="viaA"
                     /locus_tag="ACIYSB_000471"
     CDS             complement(84148..85599)
                     /gene="viaA"
                     /locus_tag="ACIYSB_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGC5427591.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(85593..87089)
                     /gene="ravA"
                     /locus_tag="ACIYSB_000472"
     CDS             complement(85593..87089)
                     /gene="ravA"
                     /locus_tag="ACIYSB_000472"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGC5427592.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            87312..89180
                     /gene="kup"
                     /locus_tag="ACIYSB_000473"
     CDS             87312..89180
                     /gene="kup"
                     /locus_tag="ACIYSB_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGC5427593.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            89347..89766
                     /gene="rbsD"
                     /locus_tag="ACIYSB_000474"
     CDS             89347..89766
                     /gene="rbsD"
                     /locus_tag="ACIYSB_000474"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGC5427594.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            89774..91279
                     /gene="rbsA"
                     /locus_tag="ACIYSB_000475"
     CDS             89774..91279
                     /gene="rbsA"
                     /locus_tag="ACIYSB_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGC5427595.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLYGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            91284..92249
                     /gene="rbsC"
                     /locus_tag="ACIYSB_000476"
     CDS             91284..92249
                     /gene="rbsC"
                     /locus_tag="ACIYSB_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGC5427596.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            92274..93164
                     /gene="rbsB"
                     /locus_tag="ACIYSB_000477"
     CDS             92274..93164
                     /gene="rbsB"
                     /locus_tag="ACIYSB_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGC5427597.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            93290..94219
                     /gene="rbsK"
                     /locus_tag="ACIYSB_000478"
     CDS             93290..94219
                     /gene="rbsK"
                     /locus_tag="ACIYSB_000478"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGC5427598.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            94223..95215
                     /gene="rbsR"
                     /locus_tag="ACIYSB_000479"
     CDS             94223..95215
                     /gene="rbsR"
                     /locus_tag="ACIYSB_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGC5427599.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(95181..96608)
                     /gene="mdtD"
                     /locus_tag="ACIYSB_000480"
     CDS             complement(95181..96608)
                     /gene="mdtD"
                     /locus_tag="ACIYSB_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGC5427600.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(96631..97323)
                     /gene="yieP"
                     /locus_tag="ACIYSB_000481"
     CDS             complement(96631..97323)
                     /gene="yieP"
                     /locus_tag="ACIYSB_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGC5427601.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT        23714 a        25580 c        24591 g        23555 t
ORIGIN      
        1 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
       61 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      121 acgttacatt gccggtgttg gcgcagaata cactgatgct ccggctttgc agcggattga
      181 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      241 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      301 ttgcgaccct catgaaatcg tcaacgtgat gggcgaagcc ggattcgcct ggtttgcccg
      361 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      421 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      481 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      541 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      601 gggtttgggt ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      661 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
      721 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
      781 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
      841 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
      901 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
      961 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1021 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1081 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1141 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1201 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1261 tgtgagcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1321 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1381 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1441 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtacaaagtc atctgccgtt
     1501 acccattgcc gggctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1561 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1621 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgg
     1681 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaaagc ccggcgtcat
     1741 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     1801 ggcggatttt gcgttcttcc attaccgcca ctatcgccat caggcagata caaccaatcg
     1861 cggcgatatc cagcgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     1921 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     1981 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2041 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2101 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2161 cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca
     2221 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2281 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2341 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2401 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2461 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2521 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2581 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacacct gctgcgcctg
     2641 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     2701 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     2761 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     2821 tgaagcccag catacaaata gcagagagga tcagcatgaa cggcaggaat tgtttggtgt
     2881 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     2941 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgaaatc atatcgttct
     3001 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3061 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3121 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3181 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3241 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3301 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3361 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3421 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3481 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3541 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3601 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3661 tcggttgccg ttaaacaatt tgtccgagcc ccaaccggct accagcgcac cgataaatcc
     3721 gcccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagtgtctc
     3781 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     3841 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     3901 acgcgtcaac cctgcccctt cttgttgttg agcaatttcc agcgcgtcgt gtcgccattc
     3961 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgt agccgccagc agagaaaaat
     4021 ccccacgact atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgcag
     4081 cgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4141 taatgcccac caaccgccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4201 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagcacgg caaacgccca
     4261 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4321 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4381 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4441 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4501 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4561 atatttatca gtcattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4621 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4681 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     4741 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     4801 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     4861 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     4921 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     4981 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5041 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gctgcccgct
     5101 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5161 agcagtgatg gtctgaccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5221 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5281 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5341 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5401 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5461 cagcgcgata atcggcagcg ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5521 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5581 gcgccagttg attggctgag aaaccagtga cggaccgagc ggcagccagg tgttattggc
     5641 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     5701 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     5761 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     5821 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     5881 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cgcccagcaa
     5941 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6001 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6061 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6121 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6181 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6241 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6301 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6361 ccgcccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6421 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6481 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6541 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6601 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6661 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     6721 ttgtcctggc gggtaaaaaa aatacgcgct taccttaacg ataagcgcga tgttgttcaa
     6781 gccttgagcg gttactgaaa aaacaccgcg atcttgttaa acatcgtcgg atcggactga
     6841 ttacgctgca ctttcacgac atcttccagc ttatcgattt ggcttatcat ctgctccaga
     6901 cgctggtcgt cattgaccag tagccagata tggcttttgt cgctgtcctg aatcggcaga
     6961 caaagaatgc cttcaacgtt aaaagcgcgg cgggcaaaaa ggccacaaac gtgggtcatt
     7021 acgcccggat ggttgcgaac ggtgagctcc agaattacgt tgtcatgagt tgtgttttgc
     7081 atggcttatt cccccaccat ttcagtattc gccgcacctg gcggcaccat cgggtaaact
     7141 ttttcttcgg catcaatgcg cacatggatc agcgccgggc cagggcgatt gatgatttcc
     7201 tgcaatgaag cctgcggatc ggcttcgtta ttcaaatcac aggtttcgag gccgaatccg
     7261 gcggcaatct gcataaagtt gattttgccc ggataggtgg cggcaaaaac gccttgctcg
     7321 tagaacagac tctgttgctg atgcaccagc cccagcgctt cgttgttcat cagaatgatt
     7381 ttgacatcca gctgattttc actggcggtc gccatctcct gaatattcat catcaggctg
     7441 ccgtcgccgg agaaacacaa cactttgcga tccgggttcg ccagcgcagc gccaatcgcc
     7501 gcaggcaggc caaaacccat cgtgcccagc ccaccggagg tcagccactg gcgtgggcga
     7561 ttgagcggat aagcttgcgc ggtccacatc tgatgctgac caacgtcggt ggtgataatt
     7621 gcattgtcat cgacacaggc ggcaacggcg ttgatcaggc cgtaatggct taacggatcg
     7681 cacgctttcg ggattggaca cggaaactca cgctgcaaat ccgctaccaa ctggtgccac
     7741 tctgcacgcg gttgcgcttc caccagcggg atcaactgcg ccagcacgtc atcaacatcc
     7801 gcctgaatcg ccacgtgcgg ctgcttgatt ttacccagct ctgcacggtc gatatcgaca
     7861 tgaatgattt tggcattcgg acagaactgc tcggttttgc caatcgcccg gtcatcaaaa
     7921 cgcgcaccga gcactatcaa caaatccgcc tcctgcaaaa tatagttggt gctgcgcacg
     7981 ccgtgcatcc ccagcatacc cagcgacaac ggatgcgctt ttggcaacat gcccagcgcc
     8041 attaaagtca tggtggtagg cagttgcgct ttctccgcca gttcacgcac ccgtgcgggc
     8101 gcattgatca caccgccgcc cagataaagc accgggcgtt tggcagcgtt aatcatcgcc
     8161 gctgcgtcac gaatgctttc ttcgctaaag gcgggggcgg cggctttttc tgccatagcg
     8221 ggctgtgttt caatctcaaa aactgccgtt tgcacatcct taggaatgtc tatccacacc
     8281 gggcctgggc ggcctgattg cgcaatgcgg aaggcatcgc tcatgacctg cgggagttct
     8341 tcgatatgtc tgaccagata gttgtgtttg gtgatgggga tagagatgcc gtaggtgtcc
     8401 acttcctgga aggcgtcggt gccgatcatc gaggcgggaa cctgaccagt gatgcaaatc
     8461 agcgggatgg agtccagccg cgcatcggca atggcggtca ccaggttagt cgcacccggt
     8521 ccgctacagg ccatacagac cgccggttta ccgtcggtgc gcgccattcc ctgagcgata
     8581 aagcccgcgc cctgttcatg acgggccaga atatggcgga tttgcgtgct ttggcttaag
     8641 gcatcgtaaa caggcaggat agaaccgccc ggaatgcctg tcacaatctt aatgccctgc
     8701 tgttccagga aatgaacgat aaattctgcg ccggtaaagc gcttacgcgt cgatgttgtg
     8761 cccgaacttg ccatgctcca gtccttttct tctgggccga ctttccggga ggtgcttaaa
     8821 cgaaaaaccc cgcccggttt gcgccggcgg ggttttggaa tcgtgtgttg ttccagtccc
     8881 tacggcgcat tgccgacgac caccaccaca cgcacgacga ccactgcggc ggatggcgca
     8941 gttggtagta gttttgcgtt gagcatggaa gtagtcattg gggaccttgt tggttttgtg
     9001 tttaacaata tttatacaag cacagcttta caggggagac aatggaaaat ttttcagcaa
     9061 gggaaaattg aggggttgat cacgttttgt actgaattgc agataacaaa aaaccccgcc
     9121 ggagcgaggt ttcgtcagtc gcctgcggct ggtaaccgca aagcacactg tattatgtca
     9181 acactgaaag tatacgtgtt ccgcgcagaa cgcgcaattt cggcacgaat tttgacgtat
     9241 ttagtgcata gttgagtatc gatcacagtt tgcgttttgt ccaaatatta ctgtttattt
     9301 atacagtaaa cttctataat atcactgtac gcaatgtgtt atgcgggggc cgcatcgtta
     9361 cccggcgcac taagtcctgg ctgaaacggg tggtgccgtc agcgccttaa ccccgcgtga
     9421 gcacactgtg ttatgtcaac aagcacaacg tttctccttg agataccgcg tgcacaacag
     9481 ctggcaacag gcagcggaaa ggtacgtcag ctggcagtgc tcctgaacca caggagacgc
     9541 gtatgaacct ggtggatatc gccattctta tcctcaaact cattgttgca gcactgcaac
     9601 tgcttgatgc tgttctgaaa tacctgaagt aattcagatt caagtcgcac caaaggggag
     9661 cgggaaaccg ctcccctttt atatttagcg tgcgggttgg tgtcggatgc gatgctgacg
     9721 catcttatcc gccctaccat ctctcccggc aacatttatt gccgcttttg tttacatatt
     9781 ctgccgctaa acaattcccc attcctggcg tatatctggc taacattcat caatgtgata
     9841 gattcctctc ccgcatttat gggaatgcgt agtgacttat tctaattatt tttataaaag
     9901 catccgtgat aatgaaaagg caaagaaacg tcaatttgtt attgatgttg gtattactcg
     9961 tggccgtcgg tcagatggcg caaaccattt atattccagc tattgccgat atggcgcgcg
    10021 atctcaacgt ccgtgaaggg gcggtgcaga gcgtaatggg cgcttatctg ctgacttacg
    10081 gtgtctcaca gctgttttat ggcccgattt ccgaccgcgt gggccgccga ccggtgatcc
    10141 tcgtcggaat gtccattttt atgctggcaa cgctggtcgc ggtcacgacc tccagtttga
    10201 cggtgttgat tgccgccagc gcgatgcagg ggatgggcac cggcgttggc ggcgtaatgg
    10261 cgcgtacttt accgcgagat ttatatgaac ggacacagtt gcgccatgct aacagtctgt
    10321 taaacatggg gattctcgtc agtccgttgc tcgcaccgct aatcggcggt ctgctggata
    10381 cgatgtggaa ctggcgcgcc tgttatctct ttttgttggt tctttgtgct ggtgtgacct
    10441 tcagtatggc ccgctggatg ccggaaacgc gtccggtcga tgcaccgcgc acgcgcctgc
    10501 ttaccagtta taaaacgctt ttcggtaaca gcggttttaa ctgttatttg ctgatgctga
    10561 ttggcggtct ggccgggatt gcagcctttg aagcctgctc cggcgtgctg atgggcgcgg
    10621 tgttagggct gagcagtatg acggtcagta ttttgtttat tctgccgatt ccggcagcgt
    10681 tttttggcgc atggtttgcc ggacgtccca ataaacgctt ctccacgtta atgtggcagt
    10741 cggttatctg ctgcctgctg gctggcttgc tgatgtggat ccccgactgg tttggcgtga
    10801 tgaatgtctg gacgctgctc gttcccgccg cgctgttctt tttcggtgcc gggatgctgt
    10861 ttccgctggc gaccagcggc gcgatggagc cgttcccctt cctggcgggc acggctggcg
    10921 cgctggtcgg cggtctgcaa aacattggtt ccggcgtgct ggcgtcgctc tctgcgatgt
    10981 tgccgcaaac cggtcagggc agcctggggt tgttgatgac cttaatggga ttgttgatcg
    11041 tgctgtgctg gctgccgctg gcgacgcgga tgtcgcatca ggggcagccc gtttaagcgc
    11101 acgtcaccgc agcatcgtca tcagctccat gggagaacga tgctgcttta tcagatcacg
    11161 catcacccgc atatgcggtg cggagtaaga ataaaacgcc tgatagcccg cacaaagcac
    11221 gctgtctggc gtctcctgcc gagcgtgcgg gcacaggcga tgccagtcgc acgcctggca
    11281 gtcacagcgc ggttgcgggt tttcgctgcc acaccatttt tgtaaggtgg cgtcaaaaag
    11341 ctcaatcgag atacgactga tatctgcccg cacccaacac tcgaacaggg caattaaaaa
    11401 tttcccccac agctctgctg tcaccgaagc ggccgtcaga tcgccccggt catcaaattc
    11461 aagataggga atgagctgta cggagcggat gccccgctgt ttgaaattgt gataaaaatc
    11521 ggcaggttgc agagcatcct tgtactctac aaccagttta tgcctgtcct cttcagcatc
    11581 aatgacctga cttcctgtca tgcgactctc tcaataaata cgataagttg atgtatatgc
    11641 gttacagcaa acagtattgc gagagataac accgcgaggg agatcaaaaa tttgtcacgg
    11701 actacgtgaa attgggaaaa atcgctattc gtgacatcca ttaaattgcg attacgggtg
    11761 tagtgccaga ggatcagcgc cacgatggcg agaatgccaa tcgaaatcca gaataacatg
    11821 cccgcctggt gccagttgtg tttgatagcc aacgccatca acgcgccgta gcccagcatg
    11881 gtacgaaacc acgccagtga tgtgcgctcc ggctgcagtc ctgggtcggc aatgcgtctg
    11941 gctttgcggc tatccggcat acaacaccag tcccataacg atcaccgcca cgaccatcaa
    12001 tattaagcta atgattaata agctgttggt gtagggcagg tcctctttca gacgcatcgc
    12061 tttttcattg cgtaaccagc gcaagtagcc atacatcgct aacccgccgg aaaacagaca
    12121 caacaacaac gccagcagtt cgcgaatgac gggcgtggcg aaatctggcg caagttgatc
    12181 aagcccaacc cccgccgcca gaaatcctag tgcagtacgg atccacgcca gaaaagtacg
    12241 ctcattagcc agcgagaagc ggtaatcagg cgcttctccg aggcgggaaa ttttcattgt
    12301 tgctcctttg caatttcact aacgctctac ctttagcggt ttttttaaga atttcgtccg
    12361 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagctc
    12421 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12481 tccacttttc attcggccct ttctccgcac caccgccaac gtttgaaagc atcgtggcgg
    12541 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12601 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct caatcaaagc ggtatcgcac
    12661 tggccattgc tgcgctactt tgcgcattta ttggcggtat gcgtaaggaa aatcgctggg
    12721 gcattcgcgg cgcgctggtt tttggcggcg ggacgctggc tttccatacc ttgctttttg
    12781 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    12841 gttcactggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    12901 tcagaacttc tgttttttct cctccacctt caccccttgt gtcggtaggc cggtgtcata
    12961 gtcgcgtaca actggcgaat agccatcttg tggacgtgga cgaaacgccc ccatccagcg
    13021 cggccgtgca tccttacgcc acggacgcag actccattgg taactgcgga acggatcgcg
    13081 aattttgtcc atgtaatcca ataaggcgtc atgcattttg ctgcgaacgt ctgcaaaacg
    13141 gatatcatcg atcaggttat gcatttcatt tgggtcatta cggcgatcgt aaagttcatc
    13201 actggtgaag aggttgagta ccagtttaaa gtcatccgtc acccagcaac gcaccggaat
    13261 aaaaccgcca aagctgtcat gctcaatctc gtagcggtta aattccacca tcacgccgcg
    13321 tggctctttc acggcaagga tattttcccc cggcagaatc tctggttttt caatatctgc
    13381 cagcgccatc attgtcggca gtaaatcgat atgactgact ggcgtatcga cctgtcgccg
    13441 ctccccttgc ggcgaacgga tgatcagcgg aatgcgggtg atgtcgtcat acatcgccgc
    13501 ccctttactg atcagcttat gtgcgcccat catttcgccg tgatcggagg tataaataac
    13561 ccacgtattt tcacgttgct ctggcgttaa ggcattgatg acccgtccga tttggtcatc
    13621 aacaaagtca ttacaggcaa aatagagcgg atggtgataa agcccgtcat caccgactgg
    13681 cgatggcatc gcctgcgccc ataagcggtg atgttccggt ttgttagcca ggtcatcctg
    13741 tgctttctcg cccagctcgt agtaaaaatc agcgtatttc tctaaatact ccaccggaca
    13801 ggtgaacggg tgatgcggct catcatacga aaccaccatc aggaaaggct cgtctgcgcg
    13861 cgcaggctgt tgcagaaaat ccaccgcccg attgctgata cgatgcgccc aggtgaaggt
    13921 ttcgtcgata tggttcgcct gtaaatcttc gacgctgttt aggccattgc gccacaggct
    13981 aatctctttt tccgtcagtt cgctaagata gttcgcccca tcgaaccagt aatcagcgtc
    14041 ccactccggc ggacactcgc cagtgccgaa atagtcatga ccgtcgagat gccatttgcc
    14101 gatgtaacag gtgtgatagc cggcatcctt aaagtagcgc cccatagtgg agatgttttt
    14161 gcctggcgcg acgttgttgg tccacgggcc ggactggtta gcgtagatac cggtaaatag
    14221 tccggcccgt gcaggcgtac aaaccggtga acaggtgtag gcggaattaa agcgaatacc
    14281 ttccgccgcc agactatcaa tattttgcgt attcagcggt ttaccgctat agcaaccgac
    14341 catattggtg gcctgggtat cggtcatgac gaacagaaaa ttggggcgtt tcataggcta
    14401 tccttcccgt cgggagtaaa ggttacggct gggtcgtgac gatgccgcca gctgtcaaaa
    14461 atcaggtaga caatcactac ggcggcaatg aaataactga tcatcgctaa ccagcgcgta
    14521 ccgtagccgc cgaattcagc cagcccggca tagacgccaa tcatcgcgaa caggatgcca
    14581 attgacgcga tcttgacgtt tttccacggt ttcatgtcca ccgcaaacgc atctttgaag
    14641 gtgaacggcg tggcgcgcgg tttgataaaa ccgatcacca gcatcacgac cacgttgatg
    14701 cagaacgtac aggccagcac atagaggaaa tggaagtcga acttcaccag atagttgatg
    14761 gtgatgtagc tgattatgcc aatccccatc gctacttttg ccgccagcgc cgggatgcgc
    14821 gggaagaaaa agcccataat gatgatggta accagcggca cgttgtaaat gccgttgagc
    14881 tgtttcatcc agctatacag cccctgcggc gcgttggcga tccacggcgc gaccagcacc
    14941 gaaacgatag cgataaagaa accaaatttg cgcccgacgg tgaccagctg ctgcggctcg
    15001 gcattctggt taatgatgcg acggtaaata cccatactga ataacgtact ggcgctattc
    15061 agaaagccgt tgaaggtact gatcaccgca ccaaataaca ccgcgccgaa gaaacccacc
    15121 attggcactg gcagaacgtt attgaccagc gtcgggtagg ccatgtcggc tttcggtaaa
    15181 tcctgataca gatgaaacgc aatcaaccct ggcagcacca gtaccagcgg gtcgagcatt
    15241 ttcagcaccg ccgttaacag cgcccccttt tgcccttccg ccaggctttt tgacgccagc
    15301 gtgcgttgca cgatgccctg atttgtacac cagtaaaagg tgttcaccag aatcaaaccg
    15361 gtaaatgccg cgccaatcgg caagggatcg gtcgggccac cgattgagtt taatttctcg
    15421 gcgtgaacgg tggtgagttg ctcaatgccc tgcataaagc tgcccttgcc catcgcgatc
    15481 aggccaaata ccggcaccat caacccgccg ataaccagcc caataccgtt gatggagtcc
    15541 gccactgcca ttgcgcgcag tccgccgatc accgcataca aaattcccgc cagaccaagc
    15601 aaaattacca gtagccagat agccgcaccg tgagaaatct gtagcgattc cccgacgtga
    15661 aacaggctgt tgagcgccaa cgcgccggag tagagaacaa tcggcagaaa gcagacgccg
    15721 gtggcaatta ggaagcagaa gtcgatgata atacgcgtcg ttttatcata acgttcctcc
    15781 agaaaatcgg ggatggtggc aatgccgcgc ttcagatagc gcggtaaaaa gattagcgcg
    15841 aggaagatca gcgtcaccgc tgaagtcact tcccagccca tcaccgacat gccgcttttg
    15901 taggcctgcc cggaaaggcc gaccagttgt tccgtggaaa ggttggttag cattaacgaa
    15961 gcggcaataa ccggcgcttt tagtgaacgt ccggcaagaa aatagccttg ttgcgatccg
    16021 gtatctgttt tgcggacctt ccaccaggtg atcaccgcca ccagcagcgt aaaaccgaca
    16081 aaactcaaga tttgtaacga attcatctca tagcccttat ttatgtttat gtcccggacg
    16141 catgaatata gggagaggta aaatgcgtcg ggtcaggcag tcagattctt aaaatacaaa
    16201 cgtcgtatcc ctgaacggat tgttttctgt taagttcagg ttgtgagcat aattcgccgg
    16261 agggatatta aaatgaatgg aaaattgcaa agttcagatg taaaaaacga aactccgtac
    16321 aatattccct tattgattaa cgaaaatgtg atctccagcg gaatttctct gatctcgctc
    16381 tggcatacct acgccgacga gcattaccgg gtgatctggc cgcgggacaa gaagaaaccg
    16441 cttatcgcca actcatgggt tgcggtgtat accgtacaag gatgtgggaa aattctttta
    16501 aagaatggcg aacaaataac actgcatggc aactgtatta tattcttaaa gccaatggat
    16561 attcactctt atcactgtga aggtttagtc tgggaacagt actggatgga atttaccccc
    16621 accagtatga tggatattcc cgttggtcag caaagcgtta tttataatgg cgaaatttat
    16681 aatcaggaac tcaccgaagt tgctgagtta ataacttcac cagaagcaat aaaaaataat
    16741 ctggcagtcg cttttctgac gaaaattatt tatcagtgga tttgtcttat gtacgcagat
    16801 ggtaaaaaag atccacaacg gcggcaaatt gaaaaattaa ttgccacttt acatgccagt
    16861 ctgcaacaac gctggagcgt agctgatatg gctgccacga tcccctgtag cgaagcctgg
    16921 ttgcgtcgtc tgtttttacg ctataccggc aagacgccga aagaatatta cctcgatgcg
    16981 cgtctggatc tggcgctatc gctattaaag caacaaggaa actcggttgg cgaagtcgct
    17041 gatacgctca acttcttcga ctcctttcat ttcagcaaag cctttaaaca taaatttggt
    17101 tatgcgccgt cagccgtgct aaagaatacg gaccagcacc caacggatgc cagtccacac
    17161 aattaacgta actctggcca gtacgctttg ttggcctcca gcaactcatc cagaatagct
    17221 ttggcgaccg aggcgctcgg taccgttttc gacaacgtaa tcgcctgcca caggtgctga
    17281 tatgaacgct gttcccaggc gtccacgacc agtttttcca ccgccacttg ctgactcatt
    17341 aaccctttct gaaactgcgg gatatcgccg accaccagtg gttcaggccg tagtagcgca
    17401 cgcgcatctg tttgcgatct tgcagcccaa caatttgcgc catccggctt tcaataccga
    17461 ttggcatgtc acagatgttg aggattttcg cattcgggcg cagacgacgc gtggcttctg
    17521 cgacaatggc tgccgggttg gagtagttga gcatccaggc atttggtgaa tatttttcca
    17581 tataatccac cagttccagg acgccgccaa tggaacgcat gccgtacgct attccgccgg
    17641 gtccgcaagt ttcctgacca acaacgccgt ggcgcagcgg gattttttca tccagttcgc
    17701 gcatcgggta tttgccgacg cggatgtgcg ccatgacaaa atcaacgtcg ctgaatgcca
    17761 cttcaggatc ggtggtgtaa ctaaacgcaa tgtccggcgc tttttcttta aggatgactt
    17821 tacaggcttc ggcaatcact tcctgccgcg caccatcgtt atcataaaat ttcagtgcac
    17881 gaagcgggaa acggtcctga ttcgccagga gcatcaacac gatgcctggc gtaaaggtgc
    17941 ttccaccgcc tgcgacaacc actgagaatt tggtcattat actgcctccg taatggcaac
    18001 attttctgct gattgatgag aattaattaa tctatcgagc tgttcacgca gctgagatac
    18061 atgcagacca atgatcacct gaatggcatc gccactacgg aagacgccgt gcgctcccag
    18121 ctttttaaag acttcgtcat ccagcgtttg tgacatgtca tgcagtgcaa tacgtaaacg
    18181 cgtcgcgcaa ttgttaatgc tggagatatt gccgaccccg cccagggctt gcaggatacc
    18241 ggcagcctga tccagctctt tttttggctc tgccgcggtg gtttggcctc gcgaggcttt
    18301 gtattcggct tttgagtaga gtttcacttc cgcatcttca cgtcccggcg tgcacatgtt
    18361 gaactgcaga atcagtgtgc ggaaaaccac gaagtacagc agggtaaagc acaacccaat
    18421 ggcgatttgg gtcagcatca tatccgcgtg gttgctgaac atcgggatcc agttttgcgg
    18481 taaaacctgg tcaatcagac ctccgcccat gttgcccacc acaccaaaga gatacattac
    18541 ggtcgacatt gaggccgcca gcacggcgtg taccgcaaac agcaacggtg aaatgaacag
    18601 gaaggtaaat tccagcggtt cggtaattcc caccagcatg gcggttaagg ttgcgggaat
    18661 cagcaagccc gccactttta cccgattttc cggtgcggca gtgaagtaca tcgctaaaga
    18721 aatgcccacg gcaccaaaga tttttgagtt accgtgcagg gcaaaaccgc cttccgggaa
    18781 caacgatttc agcggctcgg cgctcaaact gaactcttgc agatgctgcg cccagtacat
    18841 ctgaatgccg ccttcaacag ctgccggacc aaagataaac tgtccgtaga tgaagtggtg
    18901 taaaccggtt gggatcagaa tacgttcgag gaaggtgtaa acccagaccc caagtgcacc
    18961 cgccgaacgc aggaacgctt gcagagattc aatccccatt tgtacttttg gccagccgag
    19021 caacgtcagc caggcacagg ggatcatcac caggaaggcg ataatcacca cataagacgt
    19081 cccctggaaa atgccgagaa aaaccggcag ttttttatcg aacagacggt tatgcagcgc
    19141 cgtcacaatg ccggaaatga taattgcgcc gataatgctg gtatcgaggg ttttaatccc
    19201 ggccatcatt gtcagaccgc tacctgccac cgcgtcctga gtgaaatcga cgccgaagta
    19261 gcttccccag gtcattccca tcgcgttgat gaaatagttc caggtcagga aactcaccat
    19321 caccgccaga caagcacgcc cctgcgcttg cttagcaagg ccaatgggta aaccgacagc
    19381 aaaaatcagc ggcatattac ggaataccgt ccaaccgccc tcttcaataa tgtgtacgat
    19441 ttgcgcgaat aaactgttcg gatcggtcag tgattccccg acaaacatcg ggttttgcag
    19501 caagatggca agacccacca caatcccggc gaagggaaac agcagcactg gcgtgaacat
    19561 cgcgccgcca aagcgttgaa tttgactgag catttccaca tcctcttttc tcaattctgt
    19621 aagtaggaag cctgattggt tatgcggcag gcctggagcg aggataggaa gatgcgatcg
    19681 gcagaacatg acgacccaat gggattcgtg atcgctttca tgcttttcgt tgggcggtag
    19741 agatctacgt tttcgcgaaa aagtagacct ctgtgagcac aggtacacag catcgaacac
    19801 atctttaaaa aaaagatgtt ttttcaatcg attaagcaga acttgtgtgc gcattacccg
    19861 ggcttgcagg caaaaaagag atctagagat gatctacaaa agcattgcgg agcggttaag
    19921 aattcgactt aactccgcag atttcacgct aaacagcctt cttcccggtg aaaaaaagct
    19981 ggcggaagag tttgcggtat cgcggatgac catccgtaaa gccattgacc tgctggtagc
    20041 gtgggggctg gtggtccgcc gccacggtag tggcacttac ctggtgcgca aagatgtact
    20101 gcatcaaacc gccagtctga ccgggctggt ggaggtgtta aaacggcagg gaaaaacggt
    20161 caccagccag gtgctgattt ttgaaatcat gcctgcgcct ccggccattg ccagccagtt
    20221 acggattcaa atcaacgagc agatctactt ctcccgtcgc gttcgttttg tggaagggaa
    20281 accgctgatg ctggaagaca gctatatgcc ggtaaaactg ttccgtaatc tttcgctgca
    20341 acatctggaa gggtcgaagt ttgaatatat tgaacaagag tgcgggattt tgattggcgg
    20401 taattatgaa agcctgacgc cagtgctcgc cgatagactg ctggcgcggc aaatgaaggt
    20461 agcggaacac acgccactgc tgcggatcac ctcgttgtca tatagcgaga gcggggagtt
    20521 tttgaattat tcagtgatgt tcagaaatgc cagcgaatac caggtggagt accatttacg
    20581 gcgactccac ccggaaaaga gttaaccgat actccagaag agcaccgcca gtaattgggg
    20641 ggtgataatg cgcaggaaca ttaccaacgg atagacagtg gcgtaagaga gcgccgccgc
    20701 accgctggtt ggatgaagat tattagcaaa cgccagcgcc ggaggatcgg tcatggaacc
    20761 tgccagcatc ccgcacatgg tcaggtaatt cattttggct aacatccgcg ccagaatgcc
    20821 aacagtaatc agcggaacgg cggtgatcag ggcaccataa ccaatccagc ttagcccttc
    20881 gccattgacc agggtattca caaaatcccc accagatttc agaccaacga ccgagaggaa
    20941 cagcacgatc cccagctccc gcagcgcgag gttggcgctt ggcggcataa accagtacag
    21001 cttgccgata ctgccgatac gcccgaggat caacgccata atcagcggtc cgcccgccag
    21061 ccccagtttc aacgcggccg ggaatcctgg cacaaagacg ggaatagaac ctaacaatac
    21121 gcctagcccg atgccaataa acactggcag catctgaacc tgttgcagtt tttgctgcgc
    21181 attccccagc acattggcaa cggcatcaat tgcggacgga cgccccacca gattgaggat
    21241 atcgccgaac tgcaggctga tatcgccact ggcgaccagt tcgaccccgg cacggttcag
    21301 gcgcgagata acaacgtcat agcgttcttt aaagtgcagg tcgcgaatac gttttccgag
    21361 cacgttttca ttggtgacca ccacacgctc gacgcgcaaa tcagtgcctt tcgtggacag
    21421 cgaagtatcg acctcctgac caatcaccag ttgcgcatta tgtaaatccg ctggctgacc
    21481 caccagatgc agcaaatcgc ccagttggat aatggtatct ggcgaaggaa cttttagggt
    21541 ttcttcgcgt ttcagacgcg agcagataat tttgtcgccg ttgagaatcg gtacatcttt
    21601 aatggctaaa tcatgcaggt tagggttctc aacgcgaata ttgatagtct tgatcagcgc
    21661 gccgccattg gtgcgtgaag actcgtgctg ctgagcttct gtctcgacat tgacgcggaa
    21721 aataacccgc aacatccaca tggtgaacaa aatcccgcaa atgccgaatg gatacgccat
    21781 cgcgtaactc atccccatct gatcgaccat ttccattggt gtacccaggt cgcgcaaaat
    21841 ctgctgccct gcccccagcg ctggcgtatt ggtaaccgca ccggagaaaa tccccagcac
    21901 taccggcagt ggaatatcaa acagtttatg caggatggcg gtaaccagac caccgatgat
    21961 gacgatcaga acagcaaaca ggttgaggcg taatccggag acgcgcaatg aggcaaagaa
    22021 gcccggccct acctgaatcc cgatagtata aacgaacagg atcaggccaa attcctgaat
    22081 aacatgcagc atatcgctac ttaatgtcat ccccgcctga gaaacaaaat ggccgacgat
    22141 gatcccacca aacagcacgc cgccaatacc taatcctatg ccgcgaaatt tgacgttgcc
    22201 gataaacaaa ccgacgactg ccaccaaagc cagaatactg accgttaatg ctatatcact
    22261 catcgtccat ttctcttgag ataacatttt aattgctacg gattatggct gaatcggtga
    22321 agcagatttg ccttatagcg cacattatca cgggaaagaa cgtgccgaaa tatcttaaac
    22381 agtcgaaatt taacgcattg ttgagtaagt aagacaaaaa aagccccacc agaatggcgg
    22441 ggcaaagaga atagctagtt agctatttaa cgcgggacgt tcgctgatag cgatacgctg
    22501 cgctgcgatg ggttcaggct cattacgaat taaatcaata tgcagtaaac cgtttacgaa
    22561 ggttgcgcca gagacttcca tattttcagc cagcgtaaag ctcaggctaa atggctgatt
    22621 cataagccct tgatgcagcc attttttctc ttcttttggc tgctccggcg tgccttttac
    22681 gctcaggcgc gtaccttcca gttgaatctc taaatcttcc tgacggaaac ctgccagcgc
    22741 aagggtaatg cggtagtggt tatcgtcgct tttctcaatg ttgtacggcg ggaagctctg
    22801 gctttcaccg gcgttttgca gtgcgttggc cagtttgtca aaaccgatcc attgacgcat
    22861 cagtggggat aaatcgaagt tacgcatagt catttctcct tctaagaagc gagtaagtac
    22921 ctgcaaatcc gaagattcgc atatgctccc tgacggcgag catggagatg tcaggccgcg
    22981 ccaggcggcc ttagggaatt agttgatttc gatacggcgc ggttttttcg cttccggaat
    23041 cacgcgttcg agatcgatat acagcaaacc atttaccagg ttagcaccac gaacatgaat
    23101 gttctcagct aactggaatt tgcgttcaaa gttgcgttca gcgatgccct ggtacagata
    23161 ggtgcgctct ttttgttcgt cggcgtgagc acctttcacc accagcagat tatcctgggc
    23221 ggtaatttcc agttcgctct cagcaaaacc agccacagcg atagcaatgc ggtaatggtt
    23281 ttcgtctacc agttcaacgt tatacggagg gtagccgcca ttactctggc tctggttgtt
    23341 ttctaagtgg ttaaacaaac ggtcaaatcc aatagcagaa cggtaaagcg gggataaatc
    23401 aaagttacgc ataatcaata gctcctgaaa tcagcgagaa tgtaagacct tccacaatgg
    23461 acaggtcagg tagccagaac acccatcagg cgcgtcctca tcggctacga tgtaaaaatg
    23521 ggtctggaaa tgaacttttc aagccttatc agcggacttt tttttgcagt ttatggtcta
    23581 ttgcatagac tgagggggca gcacgaaacg ttaaagtgcg atagccgcca cagtgcgacg
    23641 taatgatgat gttattttca gcaagggatc gctataactc atcaggccaa cacccacgat
    23701 caacagatga atttattatg ataagaaatg tgttgttagc gttcatgata tgcagcggaa
    23761 tgacattact cggaggatgc tccagcgtga tgtcccatac cggagggaaa gaaggaacgt
    23821 atcccggcac ccgcgccagc gcaaccatga ttggcgacga tgaaaccaac tggggcacta
    23881 agtcgctggc gattctcgat atgccgttta ccgctgtgat ggacacgctt ttactgccgt
    23941 gggatgtgtt ccgcaaagac agctcggtgc gctcgcgcgt tgaaaaaagc gaagccaacg
    24001 cgcaagccac caatgcggtt attccaccgg ccagaatgcc tgataactga tttagcgatc
    24061 agtttcggtg atccacagct ggccgccttc catccacgcc agccactgac catccggcga
    24121 gaagaccacg gcgtccgcag aaggtgaatt ggcgtggtgt tcggttaaat actcaacctc
    24181 gccgctttgc gcatgggcac aagcaattcg attatccagc acaaagccca accattctcc
    24241 tgacggatgc cagttaaatg cagactgaat atccgttttg ttatgggtta actggcgcgg
    24301 ctcgccgccc tgtggcgaga taagccacag ttgcacaatg ccgttatcat ctcgcattaa
    24361 aaacgcgatt tgtgtaccct gcggattaca gcgcacccag tggcgcggga cgttgactaa
    24421 ccccggataa gcccgatggt gggtaaaggt taaacgtcgc tgcacgacgc cacgcggtgg
    24481 cgcgggcagg gttgtttccg ttccgcttaa cggcgcatca cctgccgctt tccagccagc
    24541 ttcatcttgc ggtaactcaa cgataaacag ctccggcact ttctcgccct ttggcgaaag
    24601 tgtgtcgcca ataaatgcca gcgcgtgatt tcctacccat ccttcttcat aagcacgatt
    24661 gatttcatca ctgccaggct gtggcgtggg cgtggttttg ctcaccagca cgcaccagtg
    24721 gctaccgctg tattcacgcg gatgctgctt ttgtacgttg accgggccaa acggcgcagc
    24781 aacaccgacg tttcgcaaat ccagcgccgg atcgagttca tgcattacat ggtcgttata
    24841 ggtaaagctc accctttcac cgttcgggct aaagacatgc acatggctgc cgccgcgcag
    24901 cactcctggc gtatacggcg cggtaatatc catcgcatca aggttactca tcttgccgcc
    24961 ttcaacaatc actccgcgcc gatgatggaa gtcgtaatgc catgtttcat caggattttc
    25021 cgggccgtgg atgaaaacat atttctctga cttcggatga acggtcacca cgccgacgtg
    25081 tgcgccctgt gacgcgcgat agataacctc gacctcgccg gtatggatat tcacacgctc
    25141 aatggtctcg ccggtaaacg acgcgccaga aggacgcacg tcaaatacca gccactggct
    25201 gtcgggcgtc caggtattgg tattggtgag caggtgatta cggggagcaa aggttatctg
    25261 tttcatggcg cggtcctgat acaaaacggg tcccgccatc atacttcaca aggacttcac
    25321 tttcagacaa tagtcttttg ccatcgtgat gaatgtgagt gaaaagatgg aacatttcga
    25381 cgtggcgatt attggcctcg gcccggcagg atcggcgttg gcacgaaagt tagcaggcaa
    25441 aatgcaggtg atcgcgctgg ataaaaagca ccagtgtggt actgaaggtt tcagcaaacc
    25501 ctgcggcggt ctgctggcac cggacgcgca gcgatctttt attcgcgatg ggctgacgct
    25561 tcctgtcgat gtgatcgcta atccgcagat tttcagcgtc aaaaccgtcg acgtcgccgc
    25621 atcgctcacg cgtaactacc agcgaagcta tatcaatatt aatcgccatg ctttcgactt
    25681 gtggatgaaa tcgctgatcc ccgccagcgt tgaggtttat cacgacagcc tgtgccgaaa
    25741 aatctggcgt gaggatgata aatggcatgt catttttcgt gcagacggct gggagcaaca
    25801 tattaccgcc cgctatctgg tcggtgccga tggcgcaaac tcgatggtgc ggcgacatct
    25861 ctacccggat catcaaatcc gtaaatatgt cgctatccag cagtggttcg cggagaaaca
    25921 tccggtgccg ttctactcct gcatctttga taattcgata actaactgtt attcatggag
    25981 tatcagcaaa gacggttatt ttatctttgg cggtgcctat ccaatgaaag acggtcagac
    26041 gcgtttcacg acgctgaaag agaaaatgag cgcctttcag ttccagtttg gtaagacggt
    26101 gaaaagcgaa aaatgcacgg tgctgtttcc ctcgcgctgg caggattttg tctgcggtaa
    26161 ggacaacgcc tttctgattg gtgaagcggc gggatttatc agcgccagct cgctggaggg
    26221 gattagctat gcgctggata gcacagacat tctgcgttcg gtgttactga agcagccaga
    26281 gaagctcaat accgcttact ggcgcgccac ccgcaaactg cgtttaaaac tcttcggcaa
    26341 gatagtaaaa agccgatgcc tgaccgcacc ggctttaaga aagtggatta tgcgcagtgg
    26401 tgtggcacat attccacagt tgaaggatta gccaacgcgc ttcacatcgc ccaccagcag
    26461 gatataagag agcgcgccaa tcaacgcgac ggcggagata taaaccagtg caggtgcgaa
    26521 accgtaaccc tgcgccaagt accccaccac cagcggaacg gtgatgccgc ccagaccacc
    26581 ggcgaagtta aacacgccgc cggttaaacc aatcaggcgc atcggtgcca gagaagaaac
    26641 cagcgaccag gtaatcgaag caaaaccgtt accgaagaat gccagcgcca tcaggcacat
    26701 aatcatcatc ggatcgttag tgtagttagc gcccataatg caggtggaga tcagcaagcc
    26761 gcagataatc ggcgttttac gcgcaaagcc cagtgaaaag cccttacgta ccagcagatc
    26821 cgctacccag ccagagagca ggacgccgac aaacgccgcg aggaatggca ccgtggtcat
    26881 aaagcctgct ttcagcgccg tgattccttt ttcctgggtt aaatagttcg ggaaccaggt
    26941 taagaaaaac cacagtgtag aagccaccgc aaattgccca agataaacgc cgatcagttt
    27001 acgatggaac accagtttcc agtctttggc tgttaacggc tgacgcgcct ctttcttcac
    27061 cggcgcatca ccatccacca gaccgccgcc atcacgaatg taatccagtt cagctttgct
    27121 gatacctttg gtcaggcgcg gcggctgata aaccttaaac caaatcagcg accagataat
    27181 gccgatacca ccagtgacaa tgaacaccca gtgccagctc aacatctcct gaatccagat
    27241 cagcagcggc gtcagaaacg ccagaccgac aaactgacca gacgtataaa aaccaacggc
    27301 agaagcgcgt tcatgttccg ggaaccagct ggtcaccatc cggttattgg tcgggaaagc
    27361 aggcgcttcg aaaataccgg ttatcgcgcg cagaccaatt aatgacatta agcccgtggc
    27421 aaagccctgg aataaagtcg ccactgacca gccaaatatc gcaataaaat aagtcacgcg
    27481 agaacctacg cgatctaaaa accaaccgcc ggggatctga catagcgtat aaagccaggc
    27541 gaaggccgaa aatacatagc ccatttccgc tttggtaatg ccgaactctt cctgaatatg
    27601 ggcggaagcc acggccaggt tggcgcggtc gacataacaa atgactaccg taataaagat
    27661 catcaccagc gtcagataac gccgacgccc cggctttgct gcattaacgg gaatatccat
    27721 agcgagcttt ctccagattt tgggcatagc gaagccgctc accatgccct gtaatttaca
    27781 gagggttata tttttgtatt gctgttttag tgcccgatga ggggcttacg tggcaggaat
    27841 taccactctg ctacgctgtt atcttcatga cgccagagcg gattacgcca gtccggggca
    27901 tttttactga actcaatcac tttagcttcg tcgatttcca cgcctaagcc cggtttcgtt
    27961 aacggtttaa agaagccgcc gaccatgctg aagtcttctt tgtttttcac aaagtcgagt
    28021 aactccgcgc ctttgttgta atgaattccc atactttgtt cctgaagtac ggcgttatag
    28081 gaaacaaagt cgatatgcag gcaagccgcc agtgcaatcg gtccgagcgg acagtgcggc
    28141 gcaagggtca cgtcataggc ttctgccatt ccggcgattt tgtagcattc ggtaataccg
    28201 cccgcgtggg agagatccgg ttgcagaatc gaaataccac ctgcctccag cacgcgttta
    28261 aaatcgaagc gtgagaacat gcgttcaccc gccgccagtg gaatatgcgt ttgtgccgcc
    28321 agtttcgggt agtattcggc ttgttccgcc agcaccggct cctcaataaa cagcgggcga
    28381 tacggctcca gttctttaat cagcactttc gccatcggcg cgctgacgcg accgtggaaa
    28441 tcaagaccaa actcaatctg attgccaaaa gcttcacgaa tttgtgccac ggtgttaacc
    28501 gccgcatcta ccgcgcggga gttatcaatt agccccagtt cttcacaacc gttcagtttg
    28561 aaggtatcga agccgatttc gcgtagcgtt ttaatgccgt cgataacatc cgccggacga
    28621 tcgccgccaa cccaactgta ggctttaatt ttgtcgcgaa ccaggccgcc catcagttgc
    28681 cagaccggcg cattcagcac tttacctttg atatcccata acgcctggtc aatcccggcg
    28741 atggcgctca tcaggatcgg accgccgcga tagaatccgg cgcgatacat cacttgccat
    28801 aagtcattga tgcgcgatgg atcctgacca atcaaatagt cacccagctc gtgaactgcc
    28861 gcttccaccg tacgggcgcg gccttcgatc acgggctcgc cccaaccgac cacgccttca
    28921 tcggtttcaa ttttcaggaa catccagcgg ggaggtaaac gatacgtggt aattttggtg
    28981 attttcattg cactgcctct cgatacgcct taacaaatgc tgctgcctgc tgcgcggtgc
    29041 gctctacgga ttgcccggcg cgatagagat cgctgcctaa gcccgcccct gcacaacctg
    29101 cgtctatcca ctgcgccagg ttttctggcg tcacgccgcc aacggcaaag actgcgatgt
    29161 ccgatggcaa taccgctttt aacgctttga tgtattgcgg accaaaagcc gatgacggaa
    29221 atattttcag cgcctgcgcg cccgcttcga gcgcggtaaa ggcttcggtc gccgtcgcgc
    29281 agccggggca gacggtcatg ccgtagccca ccgcacggcg gatcacttca ctatggatat
    29341 tgggcgtaac gatgagctga cagcccatcc tggcgagcgc atcgacctgt tcaggtttca
    29401 gtaccgtacc tgcgccaatc aacgccttgt cgccgtacgc atcaacgatg gcgggaatgc
    29461 tttgctccca ttgtggggaa ttcagcggga tttcaaccgc gtcgaacccg gcgtcaatca
    29521 ccgcgccaac atgcgccagc gcctcgtcgg gcgtaatacc gcgcaaaatg gcgatcagcg
    29581 ggagtttagt ttgccactgc atgagcgatg ctccttatac cagcctgaaa tgccgtgtcg
    29641 cccgccaccg ccgtcacgtc gcaacccatc gcctgaaagg cttgctggta gcgcgcggtc
    29701 agcgatgttc cggcgacaag ggtgatggcg tgttgatggg ccacatagtc gcgcatactg
    29761 gcgacctctg cgccaatcaa caaaccagag agaaattcgc tgacctgttc gcggggaagt
    29821 gttcccagca catgcgaggc gcgaacttca aaaagctgcg gcaatatggc gggcgtatta
    29881 agaccacgct caaggccagc tgtgaaggca tcggcagagt tttcctgcgg cggcaaacct
    29941 gcgccaatca atgagtgatt taacagtaaa tgatgtaatt caccggtcat cacggtgcga
    30001 aaatcgttga tttgctggct atcggcctgc acccatttgc aatgggttcc gggcatgaca
    30061 taaagagagg aaggagccag agctcgcgcg ccgatcaatt gtgtttcttc gccgcgcatc
    30121 acattgtggt tatcgtcatg agagacacat aatccgggaa taatccagat attgtcgcca
    30181 actgacgtta attgttcgcc aatagacgaa aaacaggcag gaacagataa atacggtgca
    30241 actttccagc cgacgttgct gccaaccatt cctgccatta ccactggcgt tttctcttca
    30301 cgccagtcgg tcgtgacttc tgctaacacc gcagccggag attttccgtt caggcgcgtg
    30361 acgcctgctt ctgattgcct gctctccagg cagtggtcgc cctgataaag ccaggcgcgc
    30421 agattggtcg atccccagtc aattgcgatg tagcgagctg tcatgtgatt tcctttaacc
    30481 ttcgtgtcga gctggcgatc atggtaagcg ccgcctgctc tgccgcatcg ccgtcctgat
    30541 gtcgtatcgc atcgaacagc gccttatgtt cctggagcgt ttgcggcatg ttggcctcat
    30601 cgcccatcca ggttcgttca aaaaccgccc gctgcagcga actgatcgca atgctaagtt
    30661 gctgtaacac cgggttatgc accgactgca gcaccgcctc gtggtagcga atatccgctt
    30721 cgttaaacgc ttcgcggtcc tgattgttgg caatcatctc gttcagcgcc gattcaatct
    30781 gcgccagatc gctggaagtc gcgcgctctg ctgcccaacg ggcaatcgcc ggttccacca
    30841 gatttcgcac ttcgctcatg gcactgataa gccgtgggtc gtagtcattt tccagcaccc
    30901 attgcagtac gtcagtgtcg aggtaattcc actggttacg cggtgccaca aacgccccgc
    30961 gataacgttt catttcaatc agccgcttcg ccatcagcga acggaacacc tcacggatga
    31021 tgttgcgcga ggttgcaaac tcctcacaga gttccgcctc agccggaagc ggcgagcctg
    31081 gcacgtattt gccgtgaacg atctgtttac ccagcgtaat gacaatgcga tcggttttat
    31141 tgagagtcat ggagagtcct tgtgctctgt atgttcttct ctactttacc ccgatcgatg
    31201 cataacgcgg caactttgta gtaccagcgt gatgacgttc gcgtttgccg tgcgtgtaat
    31261 gtagtacaaa cttatattgt tgtactacaa tttagatcac aaaaagaaca atgcataaaa
    31321 aaatgacatg cgtcgggcag aaatctgaaa agggatatca ggcgctaaac aggagggaaa
    31381 gaagagtatg ctttcaacgg cttagctact cgtttaaagg attaatcatg aagttgaatt
    31441 ttaagggatt ttttaaggct gccggtttat tcccactggc gctgatgctt tcaggctgta
    31501 tctcgtatgc tctggtttcc cataccgcaa agggtagttc aggaaagtat caatcgcagt
    31561 cagacaccat cactgggcta tcgcaggcaa aagatagtaa tggaacaaaa ggctatgttt
    31621 ttgtagggga atcgctggat taccttatca ctgatggtgc cgatgacatc gttaagatgc
    31681 tcaatgatcc agcacttaac cggcacaata ttcaggttgc cgatgacgca agatttgttt
    31741 taaatgcggg gaaaaagaaa tttaccggca caatatcgct ttactactac tggaataacg
    31801 aagaagaaaa ggcactggca acgcattatg gttttgcctg tggtgttcaa cactgtacca
    31861 ggtcactgga aaacctaaaa ggcacaatcc atgagaaaaa taaaaacatg gattactcaa
    31921 aggtgatggc gttctaccat ccatttaaag tgcgatttta tgaatactat tcacccagag
    31981 gcattccgga tggtgtttcc gcagcattac tgccagtgac tgttacgctg gatatcatta
    32041 ctgcaccgct gcaatttctg gttgtatatg cagtaaacca ataatcagta agcgggcaaa
    32101 cgcgtttatg ctgtttgccc gcccacagat taattcagca catacttctc aatagcaaac
    32161 gccacgccat cttcaaggtt agatttggtg acaaagttcg ccacttcttt cactgaagga
    32221 atagcgttat ccatcgccac accgacgcct gcatattcaa tcattgcgat atcgttttcc
    32281 tgatcgccaa tcgccatgat ttcttccggt ttaataccta acacgtcggc cagtgatttc
    32341 acccccgtac ctttgttaac gcgtttatcg aggatttcga ggaagtacgg cgcacttttc
    32401 agcacggtat atttctcttt cacttcctgc ggaatacgcg cgatagcctg gtcgaggatg
    32461 gcgggttcat caatcatcat cactttcagg aactgggtat tgggatccat tttctccgct
    32521 tcgcagaaca ccagcggaat ggtggcaacg aaggattcat gcaccgtgta gtagctgata
    32581 tcacggttgg cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga accgacttcg
    32641 cgagagagtt tttccaggaa acgatagtcg tcatagctga gagcagtttg cgccacggtg
    32701 ctaccatcag cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc
    32761 tgttccatat gcagctcttt caggtagttg tgcacacctg catacgggcg acccgtcgtt
    32821 agcacgacat tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa
    32881 atggtgtgat cgggcagcag aagggtgcca tccatatcga tagcaatgag tttaatagcc
    32941 atgagttccc caggtagatt ggttcctgac ccatgctaac gcgattccgc tcaaaaatca
    33001 gtacaacacc cgagggaaaa gggggatgca acgcgcgttg cgtgctccct ttttgcttag
    33061 cggaagagtt tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt
    33121 ggtgacactt caccttgcgg tgagagcgca tcaataatct tcggcaattg ttctgccagt
    33181 aaactggaag ctgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt
    33241 gtgccgagcg ccgattccag ttgctcgcca ctaaccgatt gattgccctg ttgattactc
    33301 agccaggttg agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga
    33361 atgccgccct gctcctcaac ccaacttaaa atagcctgat atttccccgc atcgcctttc
    33421 agaaaggcac cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt
    33481 gttgacccaa aaagtataga tttgcgggat gataattgcg gattgcagaa ataaaaaggg
    33541 cggagatgat ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttat
    33601 ccggcctaca aaatcatgaa aattcaatac attgcaagat tttcgtaggc ctgataagcg
    33661 tagcgcatca ggcacgctcg catggttagc gccattaaat atcgatattc gccgctttca
    33721 gggcgttctc ttcaataaac gcacggcgcg gttcaacggc gtcgcccatc agcgtggtga
    33781 acaactggtc ggcagcaatc gcatctttaa cggtaacgcg cagcatacga cgactttccg
    33841 ggtccatagt ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct
    33901 ggatggagag gccgcgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg
    33961 ctaccggctg acgacgctcg ccacgttcga taaacgcatc ttcttccagc aagccacgca
    34021 gtttctcacc cagcgtgcag atacgacgat attcgccacc ggtgataaac tcgtgatcca
    34081 gcggatagtc agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt
    34141 gctcagcatt ggtgtgaaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca
    34201 gttcgctgac cagcgcgttc acccagcggg taacggtctg ctcatcagaa aggtcagctt
    34261 ccgtcaacgt cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca
    34321 tacgattgat cattttctgc gtcgcgttgt actcagatac cagtttctct aacgcttcgc
    34381 cagccaatgc cggtgcactg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga
    34441 tctggtactg atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca
    34501 ctttgtacag cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct
    34561 gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg
    34621 tcatgatgat gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac
    34681 cacagccaag cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc
    34741 gcgctttctc gacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt
    34801 tacgcccctg cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa
    34861 gcgccggatc gcgttcctgg cagtctgcca gtttgcccgg caggcccgct aagtcgagcg
    34921 cacctttacg gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat
    34981 cgataatttt gccaaccacg attttcgcgt cggttgggtt ttccagcagg tattctgcca
    35041 gcagttcgtt catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt
    35101 tggtctggga ggagaatttc gggtccggca ctttcacgga aacgaccgca atcaggcctt
    35161 cacgcgcatc gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca
    35221 tgtaggcgtt cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt
    35281 cacgctgcgg aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact
    35341 gcaacgccac ttcgacgcca ataccgtctt tttcagtgga gaagtagaag atattcgggt
    35401 ggatcggcgt tttgttcttg ttcagatatt caacgaacgc cttgatgccg ccttcatagt
    35461 ggaagtggtc ttctttgccg tcgcgcttgt cgcgcagacg aatggaaacg ccggagttga
    35521 ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg
    35581 tgaaggtttc gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg
    35641 taaccgccag cggggcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct
    35701 cgcgctggat aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc
    35761 cgtgcagacc gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa
    35821 cggtcatgat cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc
    35881 cgcgcccgtc atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt
    35941 tacagtgacc cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt
    36001 gcagaccggt gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat
    36061 ccagcccttt caggactttg atactggagg agtcataaga attcgacatc aacgtttctc
    36121 gctcatttat acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa
    36181 ttttcgtccg acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat
    36241 tgcgtcgctt ttaagcggct ggcaagcagc ccgcgacgct catcatcaag ctcagaggca
    36301 aaatcatcta tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc
    36361 gccagacgta aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc
    36421 ggcgcaccgt cggcgcgaat gcgtaagtcc gctttgtgcg ggccgtgcgc ggtgtaggtt
    36481 agctggcgat cgcgttcaaa attacgttcc agcacctcag catattctgt ctctttctcc
    36541 cagccgcgct ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatca
    36601 gccatatcgg ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc
    36661 agcgggatca gctctttatc ccacgggcgt agctgttcgt aacgtgtcac ctggcgcagc
    36721 gccgcattgc gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt
    36781 tcgttgtgaa agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt
    36841 aaagtaaacc cttctggcgt tatcaactgc attggcatca ggtgcgccag ttccgcgacc
    36901 ttatgcccgt ctgtaccgtc gatgcggact ttgctgtcgc cctgtttgtc tttggttaag
    36961 ccaatcgctg tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa cgcctcctgc
    37021 tcatggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta
    37081 tagatggctt ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag
    37141 ccgggagata aggcgagatc cgcggtttca atgttgcgga aatcgcggat caacaagcgg
    37201 gtgagggaca ttacagtctc attggcatga caacataagc cgcgctctgg ctggccgcat
    37261 cttcaatctg cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca
    37321 gcgcgttcag aacatccagc acataactga cgttgaagcc gatttccatc tccgcaccgc
    37381 tataggtaac gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcggtgattt
    37441 tcagctggtt ttcgctgaca taaagacgta cgccgcggaa tttctcgtta gagagaatcg
    37501 ccgcgcgagc aaacgcctgc ttgagcagat cgcagccagc ttccagatgt ttgtccgggt
    37561 tcttcggcag aacgcggcga taatccggga agcgaccatc caccagtttg gaggtgaaga
    37621 taaagtcgcc aacgtgggcg cgaatgttgt tgctgccaat ctgtacgcgc agcggattgt
    37681 cgccgccgtc gagcatacgc atcagttcaa tcacgccttt acgcggtacg atcaccgaat
    37741 ggcttggcaa agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggttg
    37801 ccacggtgcg cagttcttca ccttcggttt caaacagcat accatttaag taatagcgaa
    37861 cgtcctgatg cgccatagaa aactgggtcg cttcaatcag acgcttcatc gttgcctgcg
    37921 gcagggtaaa ttcgacttca ctctgccagt catcgaggtt cgggaaatcc gccgctggca
    37981 gggtagacag cgaaaaacgg ctacgcccgg agcgtaccag catccgttca ccttccagct
    38041 gcacggcaat ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg
    38101 gaacggtcgt cgctcctggc tcgtgtggct gaaccagcgc aacacgtgcc accatttcca
    38161 tctcgagatc agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac
    38221 cgagaatcgg tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta
    38281 ataaatgctc acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa
    38341 tttgaaaaat cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag
    38401 gcatgaagca ccgtcgtgtg gtcacggcca ccaaacgcat cgccaatctc cggcagactg
    38461 tggttagtca gctctttcgc cagcgccatc gccatctggc gcggacgcgc caccgagcgg
    38521 gatcgacgct tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc
    38581 tgaatattgt cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca
    38641 cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg cattggcaat gacgcggttc
    38701 agcgcccctt ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact
    38761 tcgcccggca aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacacgggtt
    38821 tccagctctg gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga
    38881 tcctcaacgc cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta
    38941 ccttccagca gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca
    39001 aaaaactgaa tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct
    39061 tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata
    39121 taaaccactt tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg
    39181 tgagttttac ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg
    39241 ttatccgcca cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag
    39301 ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg
    39361 acgttatccc aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc
    39421 tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg
    39481 gtgccgactt caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca
    39541 ttgatattat taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac
    39601 agggccagcg tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct
    39661 gtggctggta actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg
    39721 gcggactcca ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga
    39781 cgtacgtcga aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc
    39841 ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga
    39901 tcttctgttt ctcacagatt ttcccgattt atccacagga ctttccagaa ctcgctaagt
    39961 gtaaacgatc ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac
    40021 catagacaaa aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac
    40081 ccatcagccc gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc
    40141 gtacattttc cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact
    40201 ccggagtgta caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc
    40261 gtttcatttt tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa
    40321 tcgccatgaa acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc
    40381 gtgctcgtat ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc
    40441 gcgctcgtct gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt
    40501 cccagggagt tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa
    40561 cgggctggca cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt
    40621 atcggtctta cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa
    40681 cgtctgacgc gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg
    40741 gtggtggcga aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa
    40801 aaattatggc gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct
    40861 atcaacgcct gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa
    40921 gctacggaat tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga
    40981 aacgcgtatt aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac
    41041 catttgatac cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt
    41101 gctgttcgtg tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca
    41161 ggcccaacag accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt
    41221 accggccagt ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat
    41281 caacacccgt ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa
    41341 ctctacccag ccgttccagc tgttggaaac ttcaccgcag tttatttatc aggcacagag
    41401 cggtctgacc ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa
    41461 cgttgaaaaa gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac
    41521 gtataccgac gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta
    41581 ctctgtcaac gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc
    41641 gtttggtcag ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa
    41701 cttcgcactg cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtatgagaa
    41761 atacaagttc gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg
    41821 ggtggcgatg ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa
    41881 caacttctat accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc
    41941 ggtactggtt cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga
    42001 aatccaggac aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg
    42061 gttgtggttc atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg
    42121 taactggggc ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct
    42181 gaccaaagcg cagtacacct ccatggcgaa gatgcgtatg ttgcagccga agattcaggc
    42241 aatgcgtgag cgtctgggcg atgacaaaca gcgtatcagc caggaaatga tggcgctgta
    42301 caaagctgag aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat
    42361 cttcctggcg ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc
    42421 actgtggatc cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg
    42481 cgtaacgatg ttcttcattc agaagatgtc gccgaccaca gtgaccgacc cgatgcagca
    42541 gaagatcatg acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg
    42601 tctggtgctg tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta
    42661 ccgtggtctg gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga
    42721 gttttcgcta aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa
    42781 gcaccgcgca tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt
    42841 agcccaggcc acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa
    42901 agcccgtgaa gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta
    42961 tcttccgttt aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc
    43021 tggcccgaac tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt
    43081 gatcctcgac ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc
    43141 tggtgagttt tccgaacgcg cgtttcttaa cgataaactt gacttagccc aggccgaggc
    43201 gattgccgat cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct
    43261 gcaaggcgca ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat
    43321 ttacgtcgaa gcggcaattg atttccccga tgaagagatc gatttcctct ccgacggaaa
    43381 aattgaagcc cagctcaatg acgttattgc cgatcttgat gcagtgcgtg ctgaagcacg
    43441 tcagggtagt ttgttgcgcg aagggatgaa agtggtgatt gccggacgtc ctaacgccgg
    43501 taaatcgagc ctgttaaacg cgctggcggg gcgtgaagcg gcaatcgtaa ccgatatcgc
    43561 cggaactacg cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat
    43621 catcgatacc gccgggctac gtgaagccag tgacgaagta gaacgtattg gtatcgagcg
    43681 cgcgtggcag gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacaac
    43741 agacgccgtg gatccggcag agatctggcc ggaatttatt gcccgtctgc cagcgaaact
    43801 gccgatcacc gtggtgcgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga
    43861 agtgaacggt cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct
    43921 gcgtaaccat ctcaaacaga gcatgggctt tgacaccaac atggaaggcg gcttcctggc
    43981 gcgtcgtcgc cacctacagg cgctggaaca ggcagcggaa catctacaac agggcaaagc
    44041 gcaactgttg ggagcctggg caggtgaact gctggcggaa gagttgcgtc tggcacagca
    44101 gaacttaagc gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc
    44161 cagcttctgt attggtaagt aaccgcgctt acgaagccgc attctgactg tcagatgcgg
    44221 cttcgcttca ttgttaccac tcctgttatt cctcaaccct ttttttaaac attaaaattc
    44281 ttacgtaatt tataatcttt aaaaaaagca tttaatattg ctccccgaac gattgtgatt
    44341 cgattcacat ttaaacaatt tcagaataga caaaaactct gagtgtaata atgtagcctc
    44401 gtgtcttgcg aggataagtg cattatgaat atcttacata tatgtgtgac ctcaaaatgg
    44461 ttcaatattg acaacaaaat tgtcgatcac cgcccttgat ttgcccttct gtagccatca
    44521 ccagagccaa accgattaga ttcaatgtga tctatttgtt tgctatatct taattttgcc
    44581 ttttgcaaag gtcatctctc gtttatttac ttgttttagt aaatgatggt gcttgcatat
    44641 atatctggcg aattaatcgg tatagcagat gtaatattca cagggatcac tgtaattaaa
    44701 ataaatgaag gattatgtaa tggaaaactt taaacatctc cctgaaccgt tccgcattcg
    44761 tgttattgag ccagtaaaac gtaccactcg cgcttatcgt gaagaggcaa ttattaaatc
    44821 cggtatgaac ccgttcctgc tggatagcga agatgtgttt atcgatttac tgaccgacag
    44881 cggcaccggg gcggtgacgc agagcatgca ggctgcgatg atgcgcggcg acgaagctta
    44941 cagcggcagt cgtagctact atgcgttagc cgagtcagtg aaaaatatct ttggttatca
    45001 atacaccatt ccgactcacc agggccgtgg cgcagagcaa atctatattc cggtactgat
    45061 taaaaaacgc gagcaggaaa aaggcctgga tcgcagcaaa atggtggcgt tctctaacta
    45121 tttctttgat accacgcagg gccatagcca gatcaacggc tgtaccgtgc gtaacgtcta
    45181 tatcaaagaa gccttcgata cgggcgtgcg ttacgacttt aaaggcaact ttgaccttga
    45241 gggattagaa cgcggtattg aagaagttgg tccgaataac gtgccgtata tcgttgcaac
    45301 catcaccagt aactctgcag gtggtcagcc ggtttcactg gcaaacttaa aagcgatgta
    45361 cagcatcgcg aagaaatacg atattccggt ggtaatggac tccgcgcgct ttgctgaaaa
    45421 cgcctatttc atcaagcagc gtgaagcaga atacaaagac tggaccatcg agcagatcac
    45481 ccgcgaaacc tacaaatatg ccgatatgct ggcgatgtcc gccaagaaag atgcgatggt
    45541 gccgatgggc ggcctgctgt gcatgaaaga cgacagcttc tttgatgtgt acaccgagtg
    45601 cagaaccctt tgcgtggtgc aggaaggctt cccgacatat ggcggcctgg aaggcggcgc
    45661 gatggagcgt ctggcggtag gtctgtatga cggcatgaat ctcgactggc tggcttatcg
    45721 tatcgcgcag gtacagtatc tggtcgatgg tctggaagag attggcgttg tctgccagca
    45781 ggcaggcggt cacgcggcat tcgttgatgc cggtaaactg ctgccgcata tcccggcaga
    45841 ccagttcccg gcacaggcgc tggcgtgcga gctgtataaa gtcgccggta tccgtgcggt
    45901 agaaattggc tctttcctgt taggccgcga tccgaaaacc ggtaaacaac tgccatgccc
    45961 ggctgaactg ctgcgtttaa ccattccgcg cgcaacatat actcaaacac atatggactt
    46021 cattattgaa gcctttaaac atgtgaaaga gaacgcggcg aatattaaag gattaacctt
    46081 tacctacgaa ccaaaagtat tgcgtcactt caccgcaaaa ctgaaagaag tttaattaat
    46141 actacagagt ggctataagg atgttagcca ctctcttacc ctacatcctc aataacaaaa
    46201 atagccttcc tctaaaggtg gcatcatgac tgatcaagct gaaaaaaagc actctgcatt
    46261 ttggggtgtt atggttatag caggtacagt aattggtgga ggtatgtttg ctttacctgt
    46321 tgatcttgcc ggtgcctggt ttttctgggg tgcctttatc cttatcattg cctggttttc
    46381 aatgcttcat tccgggttat tgttattaga agcaaattta aattatcctg tcggctccag
    46441 ttttaacacc atcaccaaag atttaatcgg taacacctgg aacattatca gcggtattac
    46501 cgttgccttc gttctctata tcctcactta tgcctatatc tctgctaatg gtgcgatcat
    46561 tagtgaaacg atatcaatga atttgggcta tcacgctaat ccacgtattg tcgggatctg
    46621 cacagccatt ttcgttgcca gcgtattgtg gataagttcg ttagccgcca gtcgcattac
    46681 ctcattgttc ctcgggctga agattatctc ctttgtgatc gtgtttggtt ctttcttctt
    46741 ccaggtcgat tactccatcc tgcgcgatgc caccagcacc actgcgggaa cgtcttactt
    46801 cccgtatatc tttatggctt tgccggtgtg tctggcgtca tttggtttcc acggcaatat
    46861 tcccagcctg attatttgct atggaaaacg caaagataag ttaatcaaaa gcgtggtatt
    46921 tggttcgctg ctggcgctgg tgatttatct cttctggctc tattgcacga tggggaatat
    46981 tccgcgcgaa agctttaagg cgataatctc ctcaggcggc aacgttgatt cgctggtgaa
    47041 atcgttcctc ggcaccaaac agcacggcat tatcgagttt tgcctgctgg tgttctctaa
    47101 cttagctgtt gccagttcgt tctttggtgt cacgctgggg ttgttcgatt atctggcgga
    47161 cctgtttaag attgataact cccacggcgg gcgtttcaaa accgtgctgt taaccttcct
    47221 gccacctgcg ttgttgtatc tgatcttccc gaacggcttt atttacggga tcggcggtgc
    47281 cgggctgtgc gccaccatct gggcggtcat tattcccgca gtgcttgcaa tcaaagctcg
    47341 caagaagttt cccaatcaga tgttcacggt ctggggcggc aatcttattc cggcgattgt
    47401 cattctcttt ggtataaccg tgattttgtg ctggttcggc aacgtcttta acgtgttacc
    47461 taaatttggc taaatccttc aagaagccag ccattcgctg gcttcttgcc tctcaggaaa
    47521 tcacttatgt ccaaatggca actcgcctga tcctccttca ccacgtatgc tttgcgtcac
    47581 cttactatca ggacgcttta gcccatgtcc cgctttttga tttgtagttt tgccctggtt
    47641 ttactttatc ccgccgggat tgatatgtac ctcgttggtt taccgcgcat cgccgccgat
    47701 ctcaatgcca gcgaagcgca gttgcatatt gcgttctccg tatatctggc ggggatggca
    47761 gctgcgatgt tatttgccgg taaagtggcc gatcgttcag ggagaaagcc ggtcgccata
    47821 cccggcgcgg cgctatttat tattgcctcg gtgttctgtt cactggctga aaccagcacg
    47881 ttatttcttg caggccgatt tctacagggg ttgggcgcag gctgttgtta cgtagtggcg
    47941 ttcgctattt tgcgcgacac gctggatgat cgacgtcggg ctaaagtgct gtcattactc
    48001 aacggtatta cctgcatcat tccggtgtta gcgccagtgc tcggacatct gattatgctt
    48061 aaattcccgt ggcagagtct gttctgggcg atggcaatga tgggcatcgc ggtactgatg
    48121 ttgtctttgt ttattttaaa agaaacgcgc ccagcggccc ccgcagcttc ggataaacca
    48181 cgagaaaata gcgagtcgct gcttaaccgt tttttcctca gccgtgttgt tatcaccacc
    48241 ctcagcgttt cggtgatcct cactttcgtc aacacgtcac cggtattgct gatggaaatc
    48301 atggggtttg agcgcggtga atacgccacc attatggcgc tgaccgctgg cgtcagcatg
    48361 accgtttcat tctccacgcc atttgcgctg ggaattttta agccacgtac gttgatgatc
    48421 acctcgcagg tgttattcct ggcggcgggg atcactcttg ccgtttcacc ttcccatgcg
    48481 gtttctctgt ttggtatcac gctgatttgc gccggtttct cggtaggttt tggtgtggcg
    48541 atgagtcagg cgttagggcc gttttcatta cgcgcgggcg tagccagctc gaccttaggt
    48601 attgcgcagg tttgcggttc gtcactgtgg atttggctgg cagcggtggt tggtatcggc
    48661 gcatggaata tgctgatcgg gattctgatt gcctgtagca tagtgagcct gttgctgatt
    48721 atgttcgtcg cgcctggacg ccccgttgcc gctcatgaag aaatccatca ccacgcttga
    48781 tctcaatctg ctgctctgtc tgcaactgct gatgcaggag cgcagcgtga ccaaagcggc
    48841 gaagcggatg aacgtgaccc cttcagcggt gagtaagtcg ctggcaaagt taagagcgtg
    48901 gtttgacgac ccgctctttg tgaactcacc gctggggctg tcgcccacac cgctaatggt
    48961 cagcatggag caaaatctgg cggaatggat gcaaatgagc aatctgctgc tggataaacc
    49021 gcaccaccag acaccgcgcg gcctgaagtt tgagttggcg gcggaatcgc cgctgatgat
    49081 gatcatgctt aatgcgctgt cgaaacggat ctaccaacgt tacccgcagg cgaccatcaa
    49141 actacgtaac tgggattacg attccttaga tgccattact cgtggtgaag tggatattgg
    49201 tttttccggt cgcgaaagcc atcctcgctc gcgggagctg ttaagctcgc taccgttagc
    49261 cattgattat gaagtgctgt ttagtgatgt accctgcgtc tggttacgca aagatcatcc
    49321 ggcactgcat gaaacgtgga atctggacac ctttttacgt tatccgcata tcagcatttg
    49381 ctgggaacag agcgacacct gggcgctgga caatgtgtta caggagctgg ggcgcgaacg
    49441 cacgattgct atgagcctgc cggaatttga gcagtcactg tttatggcag cgcaacccga
    49501 caatctgcta ctggcgaccg cgccgcgcta ctgtcagtac tacaatcaac tccatcaact
    49561 gccgttggtt gctcttcctc tcccgtttga cgaaagccag caaaaaaagc tggaagttcc
    49621 ttttaccctg ctgtggcata aacggaacag ccataatccg aagatcgtct ggttacggga
    49681 aaccattaaa aacctttacg cgtcgatggc ataaccgaat cgtatgaaac gcgacccaat
    49741 ttcacaataa aatgtaaaaa agttgtaata agcttgtctg aatcgaactt ttagccgctt
    49801 tagtctgtcc atcattccag taaatgatta ctcttgtatt cataatggac cattaagcat
    49861 ggagcgaaaa atggcgactc actttgcccg agggatttta acggaaggac atctgatttc
    49921 tgttcgtctc ccctcccagt gtcatcaaga agcccgaaac attccccctc atcgtcaaag
    49981 ccgttttctg gcgtctagag gtttactcgc agaactgatg ttcatgctgt atggcattgg
    50041 cgaattgccg gaaatcgtca ccctgccgaa aggtaaaccg gttttcagtg ataaaaattt
    50101 gccttcgttt tctatttcct atgccgggaa tatggttggc gtggcgttaa caaccgaagg
    50161 tgaatgtggt ctcgatatgg aactacagcg tgcgacgcgc gggtttcata gcccacacgc
    50221 gcccgataac cacacctttt ccagcaatga atcgctatgg atcagtaaac aaaacgatcc
    50281 taacgaagcg cgggcgcagc tcatcacgct gcgccgaagc gtgctaaaac taaccggtga
    50341 tgttttgaat gacgatccgc gcgatctgca gctgctgccc attgccggac gcctgaaatg
    50401 tgctcatgta aatcatgtag aagcgttatg cgacgcggag gacgtgctgg tgtggtccgt
    50461 ggcggtcacg cccacgattg aaaagctcag tgtctgggag ttcgatggca aacacggctg
    50521 gaaaagcctg ccggatattc acagccgcgc caacaatcct accagccgga tgatgcgttt
    50581 tgcccagctc tctaccgtga aggctttttc gccaaattga tagacaacca caggagtcat
    50641 catgtctgaa aaattgcagg tggttacgtt actggggagc ctgcgcaaag gctcatttaa
    50701 tggcatggtt gcacgtaccc tgccgaaaat tgctccggca agcatggaag tcaatgcgtt
    50761 accttccatt gccgacattc ctctgtatga cgctgacgta cagcaggaag acggttttcc
    50821 agcaacggtt gaagctctgg cggaacagat ccgtcaggct gacggtgtgg tgatcgtcac
    50881 gccggaatat aactactcgg taccgggtgg gctgaaaaat gccatcgact ggctttcccg
    50941 cctgccggat caaccgctgg caggtaaacc ggtgttgatt cagaccagct caatgggcgt
    51001 gattggcggc gcgcgctgtc agtatcacct gcgccagatt ctggttttcc tcgatgcaat
    51061 ggtgatgaac aagccggaat ttatgggcgg cgtgattcag aacaaagttg atccgcaaac
    51121 cggagaagtg attgatcagg gtacgctgga ccacctgacc gggcaattga ccgcatttgg
    51181 tgagtttatt cagcgagtta agatctaaat aaaaaacccg ccagtaatca tgcatggcag
    51241 gtttttaatg cgctatcgat tttaatgagc gtcgataaat acaatcttca ggataaacat
    51301 cagcgcaacg atgattacgc acgggctaag gtcacgcaga cgcccggtac caatcttcat
    51361 cacgcagtag gagataaagc ccagcgcaat accttcggta atcgaaaagc tgaacggcat
    51421 catcacggcg gtaataaacg ccggaacaga ttcagtaaga tcctgccagt tcacgcgagc
    51481 aagacttgag gtcatcaaca cgccaacgta gatcaacgcg ccagctgcag cgtagcctgg
    51541 caccattccc gccagcggcg acagaaagat aaccagcagg aacagcagac caacaaccac
    51601 tgccgtcaga ccggtacgac cgccaaccga tacgccggaa gaggactcaa tataagccgt
    51661 aacggaagaa gtaccgataa acgaaccggt cacggaagag atactgtcga catacagcgc
    51721 ctgcttcatg cgcgggaatt tacccttctc atccgccagg cctgctttat cggtcacgcc
    51781 aatcagcgta ccggaggagt caaacaggtt gaccagcatg aaagagaaaa tcacccctgc
    51841 cagcccgagg ttaaacgacc cggctaaatc tacatggccc acgacagtca ttacgctcgg
    51901 cggcgcagaa acgatgccat tgtagtggac atcacccagc atccagccca gcagcgttgt
    51961 caccacgatg gaaaccagca ccgccgcgtg aatgttgcgc gaggccagaa tagcgatgat
    52021 gaagaagccg aggatcccca gcagcacgct gtgagaagtc aggttaccga tgctgaccag
    52081 cgtttccggg ttagcgacaa tcacacctgc attcttcagt cccatcatac caatgaacag
    52141 accgataccg ctggtgatgc ccacacgcag acttaccgga atgttggcaa tcatccagta
    52201 gcgaacgcgg aaaatcgtca gtaacagcag accaatcgca ccccagaaga ttgcgcccat
    52261 cccgacttgc cacggcaagc ccatcgcctg cacaaccaca aaagcgaaga acgcattcag
    52321 gcccatagcg ggtgccagtg caactggcag gttagcaaac agtcccatca taatactgcc
    52381 gaatgcagca atcagacagg tagtaacgaa gacggcgctg gtatccatgc cagcaacgcc
    52441 aagaatttgc gggttaacaa aaacgatgta aaccatcgtc aggaaggtgg taaaaccggc
    52501 gatcacttcg gtccgtgccg tcgtgccatg ttcgcgcagt ttaaacacgc gttccagcat
    52561 cccctggcca gaagcctggg ttgtgtgttg ttgactcatt atctatttcc gaacaaggag
    52621 ggaaaatccg tcgctatcgt ataccaaaat gagacaatat ggacggctga agataacttt
    52681 ttttcattgt atttgcttat acggcaacga ttgcgtcatg cgaattggca tgacgaaaac
    52741 gttaaactaa cgataaagga aagacatgtc ccagatagaa gcggtatttt tcgactgcga
    52801 cggtacgctg gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga
    52861 atttggtatt acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta
    52921 cgaaattatc gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga
    52981 acacgtttac cgtgcagaag tcgctcggct gttcgattca gaactggaag ccatcgaagg
    53041 ggctggagcg ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta acggcccaaa
    53101 taacaaaatg cagcattcta tgggcaagct gaatatgttg cactacttcc cggataaact
    53161 gttcagcggc tacgatattc agcgctggaa gccagacccg gcgttaatgt tccatgcggc
    53221 aaaagcgatg aatgtaaatg tagaaaactg cattctggtt gatgactcag ttgccggtgc
    53281 gcaatctggt atcgacgcag gtatggaagt gttctacttc tgcgccgacc cgcacaataa
    53341 gccgatcgtt cacccgaaag tcaccacctt tacccatctt tcgcagttac cggaactgtg
    53401 gaaagcgcgt ggttgggata ttacggcata gttcttcaca ctcccttcac ttaccccgct
    53461 taaattggcg ctcaaaggta agttaagggt gtttgatatg tctgtttcac gtcgggtaat
    53521 acatcacgga ctttattttg cggttttagg accgttaatt ggtgttctgt ttcttgtcct
    53581 ctacatattc ttcgcaaaag aaccgctggt tcttttggta ataatacagg tacttccatt
    53641 gtttctctta ttgtcgatta ctacgggagc tattcctgca ttgttaaccg gcgtgatggt
    53701 cgcctgtctg ccggaaaaga tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat
    53761 aggtggcgtc gttatcaccg agatctattg tgcagttatt gtacatatta agggcatggc
    53821 ttcctcggag ttgtttgaaa acattctttc tggtgacagt ctcgttgtcc gcatcattcc
    53881 tgcattgctg gcaggtgtgg tgatgagcag aatcattacc cgtctgcccg gattggatat
    53941 ttcatgtcct gaaacagact ctttaagtta agcgggatac tttatctttg ggctactcaa
    54001 aagcagacag gatgttacta tgactcaaaa tatcaggccg ttaccccaat tcaaatatca
    54061 tcccaagcca ctggaaacag gcgcatttga acaggataaa accgtagagt gcgattgctg
    54121 tgaacaacag acgtcagttt attactcggg tcccttttat tgcgttgatg aagttgaaca
    54181 tctctgtccg tggtgtattg cggacggttc tgctgctgaa aagtttgcag gtagttttca
    54241 ggatgatgcc agcatagaag gtgttgaatt tgagtatgat gaagaggacg aatttgccgg
    54301 tattaagaac acatatcctg atgaaatgct gaaagagttg gttgaacgca cgccaggtta
    54361 tcatggatgg caacaggaat tctggcttgc gcattgtggc gatttctgtg cttttatcgg
    54421 ctatgtgggc tggaatgata taaaagatcg cctcgatgaa tttgccaacc ttgaagaaga
    54481 ttgtgagaat ttcggtatta gaaattctga tctagctaaa tgcctgcaaa agggtggtga
    54541 ttgtcagggt tatctcttcc gctgtctcca ctgcggcaag ctgagactgt ggggtgattt
    54601 ttcgtagtta tttaaataat gagaacaggc cggagcgtaa ttcatacatc cggccttatt
    54661 tcttcagctt aaccgagcgc taattctgcg gcagcggctt tatcagcgat taccatcagc
    54721 gaaggatgca gctgcaacac ggaagctgga acatcttcgg taaccggacc ttgcagcaca
    54781 tttttcagtg cctgcgcttt acctgcgcca ctgacaatta tcagcaagtt tttcgccgcc
    54841 atgatgcttt tcggccccat cgtcacgtag ctgtcaggca ctaacgaaaa gtcgccgccc
    54901 aactcaccat gcgccacaat atcaaccatc tctccctgga tcgggaactc cacggtttgc
    54961 tcatggaaat gggtcgtatt cggcaaattg ccacaaaaat gaccatctgc acctaatccc
    55021 agcaccacca aatccaggcc gccttcacgc gccagtttct gatcatgctc gcggtagtta
    55081 tcaatagtga gcttctggat attctcttct ttgatccccg caggggtgaa aaacagatta
    55141 cgcagattgg taatcgttac accttctccc tctttgccgc gaaagggaat ttcatcgaaa
    55201 ttatagaaat agcagttatc gtaccagggc ttacctttaa ccagggtggt gaggtattca
    55261 tacatgcctt tgggcgtgct accggcggta attgccaggt taacacgacg cgtcttcgac
    55321 atataaccaa gcagatggtg tgccgcgaca cggctcattt cctggtaatc ttcggtaatg
    55381 attaatttca tcttagtgcc ttattttatt taaataattt ctgtacgaag gctgcatagg
    55441 caggccgcca gacatccatt tcatggttca ggccgggata ttcctggtaa tcaaacttaa
    55501 tttttttctg ctcaagctca gttttcagcc cggcgatatc cttgccggtt acgacatctt
    55561 tatcaccaac gaccacagta aaattacgta gttgctggtt aatagctgcc ggatcgttca
    55621 gccgggccgc gacaccttca tccggtacgg ttgtcgtggt aacaccactg aatgtggcca
    55681 gccagccaaa gctttccaga tgattcattc cggaaaccag cgcctggtac ccgccttgtg
    55741 aaagccctgc cagcgcgcga ccatcggcat ctttacggac attaaaacgc ttgctaatca
    55801 gcgggataat atcgttcatc agttcgcgat ctgccgcttt agcattcagc ggataaaaga
    55861 ctttacgtct ttcctgaggc acgaaatctt cgggaataat gcccttcgca tcggtttcgg
    55921 tatccgggat caccaccagc atcggtttaa ttttcccttc agccagcagg ttatccatga
    55981 tttgcgggat acgcccctga tcgatagcgg aacgtccggt atcaccaaaa ccgtgataga
    56041 aatagagcac tggcaaaggc tcgcccatgc cggtgtatcc tggcggggtc cagacataca
    56101 tctgacgttc agattgcaat gcgttggagt ggtaagttat ggcgatcaaa tcaccatgcg
    56161 caacagaacg cgtatccaga taactgcctg gcaccagaat catactggag ttaacctggc
    56221 gctgaggctt agtcattgcg gtgcctgtat caatgctgcg tacaccatca acattgaaaa
    56281 aatactcgta caggttgcct ttcaggatag gtgtgcgcca cgaccagact cctgcttcgt
    56341 ctttggtcat cgggtgaata ttgtccggaa ccggaacacc caccactaca gagacatttt
    56401 ttgccccagg ggcaaagtag cgaaaggtca cgctgttatc ggcattgacc tgagtcacat
    56461 actgtttcac cgggatagtg ggtgccgggc tggctggcat atccgctgct atggcccact
    56521 gagcagaaat accgctggca atagccagcg ttaaagcagc aatttttata ttcatcgtaa
    56581 tattcctttc attaagttcc attaccacca gatttcagcc tgggcaccga cagcaaactg
    56641 gtcgtcttta ttgtcttcga aggtgaagcc atccagttcg ttttccaggg ctttgatata
    56701 cgtggcgtag aaacgaattt ccggacgcga ggtcaacata ctggtattga ctttaaaggt
    56761 atggaagagc gtggttttat aaccagactc attaaattta ttactattcg catctttgtt
    56821 ttgctgggtg aaatagccca gttcaacacc tgtctggtta tattggtccc aaatataggc
    56881 cgggcgaaca accgcacgaa tagattcgaa atcagagtgt gcgcctgttt cgtagctata
    56941 aatatcgtta ccgaaggagt aaacaatcgc gttagccaca ataaaatgat caccgatata
    57001 ggcttcgccc tgcgaggtca gacgaactgc tgttccgccg gtgtgatcac cataataacg
    57061 accattaaag gtggtaaatg gacttgcgcc agcataacgg ccaaagttac tggcgataga
    57121 gttattcgca accaggaagg agaattcgtt gaagccacct ttatcaaatt tctgcgttaa
    57181 agatgtgccg aacatccagg tatctttcca gtcataatac ccgttattat cctgattatc
    57241 tttttccgat gcgctttcgt ttgccgtgac ataacgacca ctcaccatta aggtcgcttt
    57301 atcccataac gggatatctt tatagcgtaa atcaatagta ttggtattaa tctgctgttt
    57361 gttttgcagg ctgcgatcgt aatcatcgac atcttcgcga accagcgcga tatcaatttt
    57421 acccggaccg actttccagt tttccagacc tacacccgct gcggcatcag tacgctgcgt
    57481 tttccagtcc agcatctgga tttcaatttt cggcgcaccg tgtttaccca cccagaaatc
    57541 agcctctggc gcaaagggca ggaaaccttt ggtggtaacg tacatatcgg agaactgcat
    57601 aaagttctcg ccaccggcgt tatcgccaaa ccagccggta gagtactgct gaccaacgtt
    57661 accatccatc atcacaacgg catcaacccg tttgccgttt tcgttgtaga cacgttgttt
    57721 taactgcaaa tcaaaccagc cggagtattc gttaccaaag cggcccagag aaccaatcgc
    57781 ccatgattta ggtgaaccat gagaggcggt cccccaacca gaacggtaat agccgttgta
    57841 actaaagcca atttcatctt tcacaaactt actgaaatct ttcagagtca tggaagaata
    57901 aatcgagcct gtctcactgg catttttctc ttcatttttg accagtacag catcaggttt
    57961 tgcggcacta ctggtcggga acggattggc ggcataccct tgatcattcg tacttacgct
    58021 acgattcacc gtcgctggcg tatatttttt cttctcttca tctttatact ttttgagttc
    58081 gctttgcgtt tctcttaacg ctttttctaa taactcaagg cgttgttcca ccgttaatga
    58141 ttcagccaat gattgtgcag aaaaggctaa cggtgccatt aataagatgg cagaggtaat
    58201 aagatttcgt ctaaacataa tatcccttta tggtgcaaag aaagaattaa cgtatcgcat
    58261 ccagactgtt ctgaatgcga cgataattaa ggtgctttat tggttatttt tttaatgaca
    58321 gcccccgcgt tttgatcact tctgcatacc atccgaagct tttcttacgc gttcttgtga
    58381 ggcttccttc gccattatca tcacgatcca cataaataaa gccgtagcgc ttagacattt
    58441 gtgaatgaga tgcactgact aaatcaattg gcccccaact ggtgtacccc ataatatcca
    58501 caccatcggc aatcgcttca tttacctgta ccaggtgatc gtttaaatag gcaattcgat
    58561 aatcgtcctg tatcgaacca tccgcttcaa cgctgtcttt tgcgcctaat ccgttctcga
    58621 caataaataa cggtttttga taacgatccc aaagcgtatt taacagaacc cgtaatccaa
    58681 ccggatcaat ttgccacccc cactctgaac ttttcagatg cggattgggg atcatattca
    58741 gtatgttgcc ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa ccagtcatgt
    58801 aataactaaa agagatgaaa tcgacggtat gttttaaatc ttctgcgtca ctttcagtca
    58861 tctcaatggt gatattgtgg tcgcggaaga aacgctgcat atagccggga tactggccac
    58921 gcgcctgaac atcaccaaag aacatccagc gccggttctc ttccatggcc tgcaacatat
    58981 cctgtggctg gcaggtgagg gggtaaacca gcccaccgag aagcatattg ccgattttcg
    59041 cttcggggag caggctatga caggctttaa ctgcccgcgc actggcaacc agttgatgat
    59101 ggatcgcctg ataaacttcc gcctcgccac tctcttctgc cagccccacg cccgtgaatg
    59161 gcgcgtgtaa cgacatattg atttcattaa acgtcagcca taacgctact ttatgttggt
    59221 agcgggtaaa gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgcc cgattagccc
    59281 aaccgccgta gtttttcacc agcccatatg gcatttcgta atgggataac gttaccagcg
    59341 gcttgatccc cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct aaccccgctt
    59401 cattcggttc ggcttcgtcg ccccgaggga aaattcgcgc ccaggcaatg gaaatacgca
    59461 gacaggtgaa gcccatctcg gcaaataacg cgatatcttc cgggtaacgg tgataaaaat
    59521 cgatggcgac atctttgata ttctctttcc ccaggatgcg cggttccatt tttcccataa
    59581 cgccatgagg ctgtaaatct gaggtcgtaa tccctttgcc atcttccagc caggcacctt
    59641 ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat gctttcataa
    59701 ttaactcctt ttatcgttag cgaatgatgg ataacagcgg ttcacctgcg cttatctgcg
    59761 ccgtgccgtg gggtaatacg tctgtaaaat catcgctatt actgattaat accggcgtcg
    59821 tcacatcaaa tccggcctcg cgaatagcag ggatatcaaa agaaatcagc cgatcgcctg
    59881 tattgacctt gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc agttttacgg
    59941 tgtcgatacc gacatgaatc aggatctcca caccatcatc tgactcaatg ccaatggcgt
    60001 gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact tcaccaaccg
    60061 agggcagaat ggcgatacct ttacccaaca ggccactggc aaacgtggta tcagcgacgt
    60121 gaatgagcgg cacaatctct cccgtcatcg gtgaacagat accgccctgc tcaggtggtg
    60181 taataacctc tggtgttttc tcttgcgggg caccctgcgc tggctgacgt ttagcggtga
    60241 tgaaatgaag catcaccgta ccgacaaatg cgcaaccgat ggcaatgaca ccgccaataa
    60301 cgctggccca gacggtgaaa tcaatccccg ttgacgggat ggtctgcatg aaggtgaaaa
    60361 tactcggcaa accaaaggag taaacttttg tttgcgcgta gccaatgatg gtggccccca
    60421 aagccccact gatacaggcg ataacaaagg ggtacttacg cggcaggttg acgccatata
    60481 ccgctggttc ggtgatacca aacaggcccg tcaacgccgc cgatccggct accacttttt
    60541 tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc gccataatgg
    60601 cgggcattaa cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgatacaca
    60661 gcggcaccag gccccagtgc agtccgaaca tgacgaagat ttgccagaag ccgcccatta
    60721 ccgcgcccgc aaatgcagga accgcctgat aaagccagag ataaccggcg gcaatcattt
    60781 cgcttatcca ggttgatagc ggccccacca acagaaaggt gacgggtgtg ataaccatca
    60841 gacatagcaa tggtgtgaag aaatttttga ttgccgacgg taaccacgca ttaagtcggc
    60901 gttccagaat gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat
    60961 tcaacaatgt gaccggaata cccaggaaat ccagccccag cgcatccgct ttttgcccgt
    61021 tctcgaaagc agtcagaatt aatggatgca ctaacgctcc accaatcacc atggcagtaa
    61081 atggattacc gccgaagcgt ttccccgcgg tgtatcccag gattatcggg aagaaccaaa
    61141 acaaggcatc actggcgctg aataaaatta aataagtacc actttgttcg gtcgtccact
    61201 gaaaagtgag cgccagagcc agcatacctt tcaggatccc ggttgccgcc atcagaccga
    61261 tcagaggcgt aaaaatacct gaaataacat aaacaaagcg gtttagcaga ttacctttct
    61321 catcattttc cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg ttaaccgcca
    61381 ggaagacatc ggccacatgg ttacctatga ccacctgaaa ctgaccaccg ctttccacca
    61441 ccataataat accgggggtc tttttcagta cctcagcttg cgctttgctt tcatccttta
    61501 acttaaaacg taatcgcgtt gcgcaatgca tcagactcac aatgttatct gcgcccccga
    61561 ctcctgcgac tatttttctg gctaactccg tcataacttg ccctctaccg ctttgcggca
    61621 aaactccaaa aaaaaacctg aaaaaacggc ctgacgtgaa tcaagcaatt tttttcaggt
    61681 tttgcccgct tagtgcggta acaatccttt actcagtaat aatatttcag tgttctttgc
    61741 gcacgcgctc tatatttatg gctaaaaaca taatctctgc gggtgaaatt ttacgttgat
    61801 actgcaaacc aataaaaatg gcgatccgtt ccgcacattg ccatgcttgc gggtaatttt
    61861 gttttactgc ttgttgtaat gattcatcac tatcgttaat tgaagcatgt tcaagaatac
    61921 gccatgataa aaacttcaga tgcgtaacca gtcgctgata actcaagctt tcttcctggt
    61981 aattaaggct gaactgaaat tttattaatt gcagcatttc gcgcattaat tgcgtgacac
    62041 ctgcaacatc ctccatattc ccgctcattt gggcactgac cagatgcatg gcaataaagc
    62101 ccacttcatc tttcggtaac tgcacgccca accgtttatc aataatggct aacgcttctt
    62161 cccctagctg gaactctttc gggtaaagcc gctggatgtc ccacagcagc gggttaggca
    62221 gcaatacgtt ttgctgaaag cgtttaatcg caaactggca atggtcagtt agcgagatat
    62281 aaatactgtc ctgcaacttt cccaggcgct cctgcgctaa agagataata cgatcacagg
    62341 ttgccatcac ctcaggagga atatgactta agagttcgct taatcgcccg ttcagttcat
    62401 gactgctcaa ggcatactct ttttctattc cacttgaatt aattctttcg ccagggcgtt
    62461 tttgaaagcc aattccgcgc cccatgacga ctttttcccg ctgttgatca tcaataacca
    62521 ccacaacatt attgttgaga attttggtga tttgcatgtt catagcaagg acctttttta
    62581 taaacaaaaa aacccgactt caccagtatt ctctggttat gtcaggtttt gcctgcgaat
    62641 gcagtaacaa tccagtcatt tattaatggt ttttataacg aacatccagg ttcggaaatt
    62701 aatttaattg cgtgcagaga aaatagcaat gcgctattga taaaaatatg accatgctcg
    62761 cagttattaa ctttgtgtaa ttttaggaat ttataaagtt atatataaca aatcccaata
    62821 attaagttat tgggatttgt ctggtgaatt atttgtcgct atcttttccc gccagcagtt
    62881 tatccagctc atcaccgcct acatgacgga aatcctgacc cttcacgtag tagaagataa
    62941 actcacaaat attctggcag cggtcgccga tacgttcgat tgaacgcgca cagaacagcg
    63001 cggtaagtac gctcggaata gtgcgcgaat cttccatcat gtaggtcatc agttgacgaa
    63061 caataccttc gtattcctga tcgacttttt tatcttcacg ataaatacgt accgcttcgt
    63121 caatgtccat ccgcgcgaac gcgtccagca cgtcgtgcag catctggatg gtatgacggc
    63181 ccagcgactc cagacttacc agcaacggct gatgctgctg ggagaatttc tccagcgcag
    63241 tacggcagat tttgtccgcc acgtcgccaa tacgctccag ctcggcaatg gttttactga
    63301 tcaccataac cagtcgcagg tcgctcgccg tcggctgacg tttggcgata atgcgcacgc
    63361 aggcttcatc gatcgccact tccatcatgt tgacgttctt gtcgccttcg atcacgcgct
    63421 tcgccagatc gctgtcctgg ttatgcatcg cggtgattgc atcagaaagc tgctgctcca
    63481 ccatgccgcc catggtcatc acctgcgtgc ggatactttc cagttcggcg ttgaactggc
    63541 cggaaatatg tttattaaga ttgagactgt ccataacgca ctcctgaatc aaccgtaacg
    63601 accggtgatg tagtcttctg tttgtttctt cgctggcttg gtgaacagat cgtccgtgtt
    63661 gctgaactca atcaattcgc ccaggtacat aaacgccgtg tggtcggaac aacgcgcagc
    63721 ctgctgcatg ttgtgggtga cgatcaccac ggtgtaatcc tgcttcagtt cggtgatcag
    63781 ctcttcaata cgcccggtag agatggggtc gagcgccgaa cacggttcgt cgagcagcag
    63841 cacttccggg cgaatggcga taccacgcgc aatgcacaga cgctgttgct gaccaccaga
    63901 gagagagtaa ccgctctggt gcaatttatc tttggtttcg ttccacaatg cggctttggt
    63961 caatgcccac tgcacgcgct cgtccatgtc ggcacgggag agcttctcaa acagacgaac
    64021 gccaaaagcg atgttgtcgt agatggacat cggaaacggc gtcggtttct ggaacaccat
    64081 gcccactttc gcacgcagca gggcgatatc ctgagagttg gtcaggatgt tgtcgccatc
    64141 aagcagaatt tcaccttccg cacgctgctc cgggtacagt tcaaacattt tgttgaaggt
    64201 acgcagcagc gtcgatttac cgcagccgga cggcccgata aacgccgtta cctggttttt
    64261 agcgatatcc aggttgatgt ttttcagggc atggaatttg ccgtagtaga agttcaaatt
    64321 acgaacctga attttactcg gggcagtttc aaccatactc attgcaatct tttcctcatc
    64381 gtgcctgatg cacttcgcga atcaggctta ccgtaggccg gatcaggcgt tcacgccgca
    64441 tccggcaaag tgttaaccgt gtacagagtc aaaaaggcgc tcggtcggtc ccctcgcccc
    64501 tctggggaga gggttagggt gaggggaaaa ccgtgtcagc aatatcaacc gtgtttattc
    64561 ttcgcgaaaa caacgcgcgc cagaatgttc agcagcagta cgcacagggt aatgatcaat
    64621 accccggccc aggccagctg ctgccattcc gcaaacgggc tcatcgcaaa cttaaagatc
    64681 gtcaccggca ggttggcgat cggctgcatc atgtccgtgc tccagaactg gttggagagc
    64741 gcggtaaaca gcagcggcgc ggtttcacca gcaatacggg caatcgccag caggataccg
    64801 gtcatgatcc cggacaccga cgctttcagc gtgatcgcgg agatcatctt ccacttcggt
    64861 gtacccagcg cataagccgc ttcacgcagg ctgtccggca ccagtttcag catgttctcg
    64921 gtggtgcgga taacaatcgg cacctgcaac agcgccagcg caatcacgcc cgcccagccg
    64981 gagaagtgct ccatctgcgc caccacaatg gtgtaaacaa acagaccaac cacaatcgat
    65041 ggcgcagaga gcagaatgtc gttaatgaag cgaatcactt ctgccagcca ggatttacga
    65101 ccatattcag ccagataaat ccccgccata atgcccagcg gcgtaccgaa taccgtggcc
    65161 cacaaaatta acaggccgct acctgccagg gcgttcgcca gaccaccacc ttccgtattg
    65221 ggcggcggcg tcatttcagt gaacagcgcc agcgacatac cgtcgatacc gcgggtgatg
    65281 gtggacatta aaatccagat cagccagaac agaccgaagg ccatcgtcgc cattgagagc
    65341 gtcagtgcaa tacggttctt gaggcggcga cgcgcctgca ttttgcggcg ggattcagcc
    65401 agcgccgcat tggtttgcat ttcaaccata gccattagcg tgccccctca ttcttagcca
    65461 ggcgcataat cataaacttc gatgcggcga ggacgatgaa ggtaatcaca aacaggatca
    65521 ggcccagttc catcagtgcg gcaacgtgca gaccggattc cgcttccgca aattcgttcg
    65581 ccagtgcaga ggtgatactg ttgcccggca tatacagcga ggcgctgtcg agctggtagg
    65641 tgttaccgat gataaaggtc accgccatgg tttcaccgag cgcgcggccc agccccagca
    65701 tgatgccgcc gataacacca tttttggtga acggaagaac gatacgccag ataacttccc
    65761 aggtggtgca gccaataccg taggccgact ctttcatcat caccggggtt tgttcgaaca
    65821 catcgcgcat taccgccgca atgtacggaa taatcatgat ggcgaggatc acgcctgccg
    65881 cgaggatacc gataccaaat gcagggccag agaacagcgc gccaacaatc ggaatattcg
    65941 acatgatatt gccgaccggc tcctggaagt aaacggcgaa cagcggcgca aagataaaca
    66001 ggccccacat gccgtaaacg atacttggaa tggctgccag cagctcaatg gcgataccca
    66061 gcgggcgttt cagccagccg ggcgcaagct cagtcaggaa cagggcgata ccgaaactca
    66121 ccgggacggc gatcagcagc gcgataaacg aagtcaccag cgtaccgtag atcggcacca
    66181 acgccccgta gatatcgttc ggtgcatccc actctttggt ccacaggaaa gccagaccaa
    66241 atttctgaat gctcggccag gaggagatga tcagagagac aataatgcca cccaacatca
    66301 atagcacaat cagcgccgcc agttttacca gcacgctgaa aattatgtcg ccctttttac
    66361 ccggtgggtt aaaagcaggc ttggttgcag ccataagtta ctcttcagtt aaacgcgttt
    66421 acaaagtcat tttgtaatgc cggatgcggc gtaaaacacc gtacccggcc tggagtttta
    66481 ttagtacagc ggcttaccgc tactgtcttt aatattggtc ttccacgcag cgcgaacctg
    66541 ttcaactaca ctatccggca ggctggcgta atccaggtcg ttcgcctgtt tagccccggt
    66601 tttgtacgcc cagtcgaaga atttcagcac ttctgtgcct tgttctggtt tcttctgatc
    66661 tttgtggatc agaatgaacg tggtagaggt aataggccat gcatcttcgc ctttctggtt
    66721 ggtcagatcc tgagcgaagg ttttgctcca gtctgcacct tttgctgcat tagcgaagtt
    66781 ttcttcggtc ggactaaccg gtttaccatc agcggagatc agtttggtgt acgccaggtt
    66841 gttctgcttc gcgtaagcat attcaacata accaattgca cccggcagac gctgaacgaa
    66901 cgcggcgata ccgtcgttac ctttaccgcc cagaccgatc ggccatttta cggtagagcc
    66961 agtaccaacg ttgtttttcc actcttcgtt cactttcgcc aggtagctgg tgaagacgaa
    67021 ggaagtcccg gagccatctg cgcggcgtac tacagcaatg ttttgtgaag gcagtttcag
    67081 acccggattc agtttggcga tggcttcatc atcccacttc ttgattttgc ccaggtagat
    67141 gtcgccgagg gttttaccat ccagcaccag ttcgccagac ttcagccctg gaatgttaac
    67201 cgccagcacc acgccgccaa tcacggtcgg gaactggaac agaccttcct gagccagttt
    67261 ttcgtcagac agcggcgcgt cagaggcacc aaaatcaacg gtattagcga taatctgttt
    67321 tacgccaccg gaagaaccga taccctggta gttaacttta ttaccggttt ctttctggta
    67381 agtgtcagcc catttggcat acaccggcgc agggaaggtt gcacctgcac ctgtcaggct
    67441 tgcttctgca aacacagaga aagcactcat cgataaggtc gcggcgacaa cagttgcgac
    67501 ggtggtacgc ataactttca taatgtctcc tgggaggatt cataaagcat tgtttgttgg
    67561 ctacgagaag caaaatagga caaacaggtg acagttatat gtaaggaata tgacagtttt
    67621 atgacagaga gataaagtct tcagtctgat ttaaataagc gttgatattc agtcaattac
    67681 aaacattaat aacgaagaga tgacagaaaa attttcattc tgtgacagag aaaaagtagc
    67741 cgaagatgac ggtttgtcac atggagttgg caggatgttt gattaaaaac ataacaggaa
    67801 gaaaaatgcc ccgcttacgc agggcatcca tttattactc aaccgtaacc gattttgcca
    67861 ggttacgcgg ctggtcaacg tcggtgcctt tgatcagcgc gacatggtaa gccagcagct
    67921 gcagcggaac ggtgtagaag atcggtgcaa tcacctcttc cacatgcggc atctcgatga
    67981 tgtgcatgtt atcgctactt acaaaacccg catcctgatc ggcgaagaca tacaactgac
    68041 cgccacgcgc gcgaacttct tcaatgttgg atttcagttt ttccagcaat tcgttgttcg
    68101 gtgcaacaac aataaccggc atatcggcat caattagcgc cagcggaccg tgtttcagtt
    68161 cgccagcagc gtaggcttca gcgtgaatgt aagagatctc tttcaacttc aatgcgcctt
    68221 ccagcgcgat tgggtactga tcgccacggc ccaggaacag cgcgtgatgt ttgtcagaga
    68281 aatcttctgc cagcgcttca atgcgtttgt cctgagacag catctgctca atacggctcg
    68341 gcagcgcctg cagaccatgc acgatgtcat gttcaatgga ggcatccaga cctttcaggc
    68401 gagacagctt cgccaccagc atcaacagca ccgttaactg agtggtgaat gctttagtgg
    68461 atgccacgcc gatttctgta cccgcgttgg tcattagcgc cagatcggat tcgcgcacca
    68521 gagaagaacc cggaacgtta cagattgcca gtgaaccaag gtaacccagc tctttcgaca
    68581 gacgcaggcc agccagggta tccgcggttt cgccagactg tgacaaggtg atcatcaggc
    68641 tgttacgacg cacggcagat ttgcgatagc ggaattcaga ggcgatttcg acgtcgcacg
    68701 gaatacctgc tagcgattca aaccagtagc gggaaaccat accggagtta taagaagtac
    68761 cacaggcgag gatctgaata tgctcaacct tcgacagcag ttcgtcggcg ttcggtccca
    68821 gctcgcttaa atcaacctga ccgtggctga tgcgtccggt aagggtgttt ttgatcgcgt
    68881 tcggctgttc gtagatctct ttctgcatgt agtgacggta aatgccttta tcgcccgcgt
    68941 catattgcag attggattcg atatcctgac gttttacttc cgcgccagtt ttatcgaaga
    69001 tgtttaccga acggcgagtg atttccgcaa tatcgccctc ttcaaggaag ataaagcgac
    69061 gggtcaccgg caacagcgcc agctggtcag aagcgataaa gttttcgccc atccccaggc
    69121 caatcaccag cggactacca gaacgtgccg ccagcagggt atccgggtga cgggagtcca
    69181 tgatcactgt accgtacgca ccacgcagct gcgggatagc acgcagaacg gcctcacgca
    69241 gagtcccgcc ttgtttcagc tcccagttca ccagatgggc aatcacttcg gtgtcggttt
    69301 cagaaacgaa ggtatagcca cgcgctttta gctcttcacg cagcggttca tggttttcga
    69361 tgatgccgtt atgcaccacc acaatgtgtt cagaaacatg cggatgcgca ttcacttctg
    69421 aaggttcacc gtgggtcgcc cagcgagtgt gagcaatacc agtgccgcca tgcagaggat
    69481 gttcttccgc tgcctgtgcc agcatctgga ctttaccgag gcgacgcagg cgggtcatat
    69541 gaccttctgc atcaacaacg gccagaccgg cagagtcata tccgcggtat tccagacgac
    69601 gtaaaccttc aagaaggatt tctgctacat cacgttgcgc gatcgcgcca acaattccac
    69661 acatagtttt tgattccgat ttatatcgtt gtcggtcaac ctgtatgccc gttttttcgg
    69721 gcgccccgag ccttgtagag agtggggtta tttttatagt tactgcttgt gggagggaga
    69781 ttatgttatc tcctcatccc atgtgaccgg gttagccggc cagaatcact ttttctttac
    69841 cggacgacgc cagccttctt tctgagtctg cggcacacgg ctgatagcta atgcattttc
    69901 gccgacatta cgcgtcacag ttgtacccgc agcaatggtc gcgcctttgc ctactgttac
    69961 cggggccacc agctgagtgt cggaaccaac aaacacatcg tcgccgataa tggtcttaaa
    70021 tttattcgca ccatcgtagt tgcaggtaat ggttcccgcg ccgatgttaa cgttatcgcc
    70081 aatttccgca tcgcccaggt aagtcagatg accagctttc gagcctttac ccagacgcgc
    70141 ttttttcatc tcaacgaagt taccgacgtg agcaccttcc agcaactcag caccaggacg
    70201 caaacgggca aacgggccaa tggtacaggc cgctgccaga ttcgcatctt ccacaacggt
    70261 atacggactg atttcgcaat catcgccaat cacgctgttt ttaatcacgc aaccggtgcc
    70321 aattttcacg cgatgaccga gagtcacgtt gccctcgatg ataacgttag tatcaatttc
    70381 aacatcgcgc ccgtgagtta gcgtaccacg cagatcaaaa cgcgctggat cgcgcagcat
    70441 aacgcctgct aacagcagtt tttcagcctg ttcggactga taaacacgct ccagacggga
    70501 gagttgcagg cggttattca cgccttctac ttcgcttaaa cgttgcggat gaacggcgac
    70561 gatttcacgc ccttcctgat acgccagcgc aataatgtcg gtgatgtagt attcgccctg
    70621 agcattattg ttggtcagct tcgccagcca gcgtttcata tctgcgccgt tggcaatcag
    70681 aatgccggtg ttgatctcct gaatctgacg ctgctcgtcg gtggcatctt tgtgctcaac
    70741 aatgccggta actttgccgt tttcacgggt gatacgtcca taaccggtcg gatcatccag
    70801 tttcaccgtc agcagaccaa tgccaccctg cggtttagca tcacgcagac gctggagtgt
    70861 ttcgacagag atcagcggca cgtcgccgta gagcattaaa atgtcttcat catcggcaaa
    70921 gaaaggtgcg gcctgctgca ttgcatgacc cgtacccagc tgctctgcct gaagcaccca
    70981 gttaaggttg tcgtctttca gcgcctgttt tagcagatcg ccgccgtgac cgtacaccag
    71041 gtgaacgtgc gctgcgccta attcattcgc agcatcaatg acatgctgaa ccatcgcttt
    71101 cccggcaagg gtatgcagca ctttcggaag atcggaatac atgcgcgtgc ctttgcctgc
    71161 ggcaaggatc actacgctca tagcattatt caacatacgc gtcctgactg taatttgaga
    71221 acgaatttaa accgcttcac cttgaaaaaa ctacattttt ttcatcgtga aatggacaga
    71281 ggataaattg ttcaataacg gattatcccc gctgacgaaa gcgccatttt cgaccatcgt
    71341 cacgcctttt gtctgcagaa aattaaggca gtaccttaat ttaagcgtca ggtggatgtt
    71401 tttgctctta tttcgatcaa tgaataaaca ggaaaacagg ggggttttat atcagcagga
    71461 tctatgtgaa cgctattcag gacgggtcac acgcgcaaaa aaaagccagc ctgtttccag
    71521 actggctttt gtgcttttca agccggtgtt acatcgcttt tttggtcaac tcgataacgc
    71581 gcagctgcgc gatcgctttg gccagttccg cagacgcctg agcgtaatct acgtcgccgt
    71641 gagagctgct aatgtgctct tcagccttac gtttcgcttc catggctcgc gcttcgtcga
    71701 gatcctggcc gcgaattgcg gtgtcggcca gaacggtcac gttgccaggc tgcacttcaa
    71761 gaatgccgcc agacagatag ataaactctt cgtgaccgtg ctgtttcacg atgcgaatca
    71821 taccaggctt aatggcggtg agcagcggtg cgtggccagg gtagatcccc agttcacctt
    71881 cgctacccgt tacctggatt ttctcgacca gaccagagaa catttgttgc tctgcgctga
    71941 cgacgtccag gtggtaagtc attgccatat caccctccga ttaaggcgtt aaagtttttt
    72001 ggctttttcc acagcttctt cgatggaacc gaccatgtag aacgcctgct ccggcaggtg
    72061 atcgtattcg ccttccatga tgcctttaaa gccacggatg gtgtctttca gggagacgta
    72121 tttacccgga gaaccggtga atacttctgc cacgaagaac ggctgggaca ggaagcgctg
    72181 gatcttacga gcacgcgcta ccaccagttt gtcttcttca gacagttcat ccatacccag
    72241 gatggcgatg atgtctttca gttcctgata acgttgcagg atggactgaa cgccacgcgc
    72301 ggtgtcgtag tgttcctgac caaccaccag cgggtccagc tgacggctgg tggagtccag
    72361 cgggtcaacg gccgggtaga tacccagaga cgcgatctga cggctcagta ccacggttgc
    72421 gtcaaggtgc gcaaaggtgg ttgccggaga cgggtcagtc aagtcatccg caggtacgta
    72481 tactgcctgt acggaggtga tagaaccagt tttggtggag gtgatacgtt cctgcagaac
    72541 gcccatctct tccgccaggg tcggctgata acctaccgct gaaggcatac ggcccagcag
    72601 tgcggatact tccgtaccgg ccagggtgta acgatagatg ttgtcaacga acagcagaac
    72661 gtcacgacct tcgtcacgga atttctcagc catggtcaga ccggtcagag caacgcgcag
    72721 acggtttccc ggcggctcgt tcatctggcc atacaccagg gatactttgt cgataacgtt
    72781 ggagtcggtc atttcgtggt agaagtcgtt accctcacga gtacgttcac ctacgcccgc
    72841 aaacacagag taaccggagt gctcgatcgc gatgttacga atgagctcca tcatgtttac
    72901 ggttttacct acacccgcac caccgaacag accaacttta ccgcccttag cgaacggaca
    72961 catcaggtcg ataactttga taccggtttc cagcagttcc tgagagtttg acagctcttc
    73021 gtaggaaggt gctgcgcggt gaatcgccca acgctcttct tcaccgatct cgcctttcat
    73081 gtcgaccggt tcacccagta cgttcatgat acggcccaga gtcgctttac ctaccgggac
    73141 ttcaatcggg tgttcgaggt cttttacatc cagaccgcga cgcagaccgt cggaggaacc
    73201 cattgcgatg gtacgtacga taccgccgcc gagctgctgc tgaacttcca gcaccagacg
    73261 ctcattacca ttttgcacct caagagcatc gtacacgcgc ggtacggcat cctgagggaa
    73321 ttcgacgtca actacggcgc cgattacctg gacaatcttt ccagtagcca tcttaaatcc
    73381 tctacgaaat aacctgttta aaccgcggcg gcccccgaga cgatctcggt gagttcctga
    73441 gtaatgctgg cctgacgagc tttgttgtat accaactgca gctctttaat caggctgccg
    73501 ccattgtcgg tcgcggcttt catcgccacc atacgggcgg cctgctcgct ggccaggttt
    73561 tcaaccacgc cctgataaac ctgagattcg acataacgac gcagcagggt atccagcaac
    73621 gccttcggat cgggttcgta caggtaatcc caggatttat gtttcagatc atcatcatct
    73681 gatgccggta acggcagcag ctggctgatg gtcggaacct gagacatggt gttaataaat
    73741 ttgttgctga caatgtaaag cttgtccaga cggccttcgt cgtaggcctg caacatcact
    73801 tttaccggac cgatcagttc ggacagggaa gggttatccc ccatgccggt gacctgggca
    73861 acaacattgc cgcccacgga gttgaagaac gacacgcctt tcgagccgat cattgcgagg
    73921 tcgcattgaa cgcctttgtc ggtccaggtc ttcatttccg ccagcagttt tttgaacagg
    73981 ttaatgttca aaccaccgca caaaccacgg tcggtcgaca ccaccaggta gcccacgcgt
    74041 ttaacgtcgc ggtcttccag gtaagggtgc ttatattcca gattaccgtg tgcaaggtga
    74101 ccaatcactt tgcgcatggt ttctgcataa ggacggctgg ccgccatgcg atcctgcgat
    74161 ttacgcattt tggaagcggc gaccatctcc atcgctttag tgatcttttg cgtgttctgg
    74221 acgcttgcga tcttactacg tatctctttt gcgccggcca tgagcttctc ctcaatgcct
    74281 tgcggcctgc cctaaggcaa gccgccagac gttaccagga ttgggttgct ttgaaggaat
    74341 cgaggatgcc tttcagcttg ccttcgattt cgtcgttgta gccaccggtc tggttgatct
    74401 cttgcatcaa cggagcgtga tcacggtcga cgtaagccag cagagcggct tcgaagctgc
    74461 caattttcga cagttcaaca tccgccaggt aaccacgttc tgctgcgaac agaaccagag
    74521 actgctgcgc aacggacatc ggcgcatact gtttctgttt cagcagttcg gtcactttct
    74581 gaccgtggtc aagctgctta cgtgttgcat cgtcaaggtc ggatgcaaac tgagagaacg
    74641 ctgccagttc acgatactgt gccagagcgg tacggatacc accggacagt tttttcatga
    74701 tcttggtctg tgctgcacca ccaacacggg atacggaaat acccgggtta accgcaggac
    74761 gaataccggc gttgaacagg ttggtttcca ggaagatctg accatcggta atggagatta
    74821 cgttggtcgg aacgaacgca gaaacgtcac ccgcctgagt ttcgataatc ggcagtgcgg
    74881 tcagagaacc ggttttccct ttcacttcac ctttggtgaa ggcttcaacg tattcggcgt
    74941 taacacgtgc agcacgctcc agcagacgag agtggaggta gaaaacgtcg cccgggaatg
    75001 cttcacgtcc tggcggacga cggagcagca gggagatctg acggtaagca acagcctgtt
    75061 tagacaggtc atcgtaaatg atcagcgcat cttcaccgcg gtcacggaag tattcgccca
    75121 ttgcgcaacc ggcatacggt gccaggtatt gcagtgcagc ggattcagac gcggttgcta
    75181 ccacaacgat ggtgttagcc agtgcgccgt gctcttccag tttacgtacc acgttagaaa
    75241 tggtggacgc tttctggccg atagcgacat agatacattt gataccggaa tcgcgctggt
    75301 tgatgatggc atcgatagcc agtgcggttt tacctgtctg acggtcaccg atgatcaatt
    75361 cacgctgacc acgaccgatt gggatcatgg agtcaacggc tttataaccg gtctgtaccg
    75421 gctgatctac ggactgacgt tcgataacgc ccggagcgat tgcttctaca gcagagaagc
    75481 cgtcgtgatc cagcggacct ttaccgtcga ttggtgcacc cagagtgtta accacacggc
    75541 ccagcaggcc acggccaacc ggaacttcca ggatacggcc agtacactta actttcatgc
    75601 cttcggcaag gtcagcgtac ggacccataa caaccgcacc tacagagtcg cgctcgaggt
    75661 tcagtgcgat agcgtaacgg ttacccggca gggagatcat ttcaccctgc atacaatcgg
    75721 ccaggccgtg aatgcggata acaccgtcac ttacagaaac aatagtacct tcgttgtgag
    75781 cttcactcac aacattgaac tgagcaatgc gctgcttgat cagttcgctg atttcggtgg
    75841 aattcagttg catgctccag tccccttaag actgcaagac gtctgcaagg cgctcaagac
    75901 gaccgcgtac gctgccatca atgaccatat cacccgctcg gatgataacg cctgccatta
    75961 cagacttatc gattttgcaa ttcagcttaa ctttgcgtga cagacgtttt tccatcgcag
    76021 cagaaatttt cgcgagctgt tgttcactca gtgcggcagc ggaaatgacg tctacctcag
    76081 cggtagcctc actcacggca cgcaggtgaa taaactgctc cagaacatcc gggagcgcgt
    76141 taagacgacc attttcagcc ataacccgaa tcaggttctg accgttttcg tccagttgct
    76201 caccacaaac tgcgataaac gactcggcga gcgtttctgg cgcaagcgcg ccagagagaa
    76261 gctctgccat ttgttcgttt ttggttacct cggcggcaaa cgccagcatg tcctgccagc
    76321 gttctacact ttggtgttcg acggcaaagt caaaagctgc tttggcgtag gggcgagcta
    76381 ccgtaataaa ttcagacatc agcccctccc tccttacagt tcagcgacaa gtttatccac
    76441 gatgtcgctg ttagcagctt catccacgga acgttcgatg atcttctcgg cgccagcaac
    76501 agccaggata gcaacttgct tacgcagctc ttcacgggca cgtttacgct cggcttcaat
    76561 ttccgcctgc gcctgggcca cgattttagt acgttcctgt tctgcctcag ctttcgcttc
    76621 gtccagaatc tgcgagcggc gtttgttcgc ctgctcgatg attacctggg cttccgcttt
    76681 cgcttttttc agctggtcgg tcgcgctggc ctttgcaagg tcaaggtcct tatgtgctcg
    76741 ttctgcggaa gcaaggccgt cagcaatttc tttttgacgt ttttcgatgg ctgccattaa
    76801 tggcggccat acgtacttca tgcagaacag aacgaacagg acaaacgcga tggcctggcc
    76861 gaggattgtt gcgttaagat tcacagcaca atgcctctat ttagttaacg ttctgatatt
    76921 gctctttaaa taaaagcaac gcttactacg cgacagcgaa catcacgtac agacccagac
    76981 ctacagcgat catcgggata gcatccacca gacccataac gataaagaac tgagtacgca
    77041 gcagaggaat cagatcaggt tgacgcgctg cgccttccag gaatttaccc ccgaggatgc
    77101 cgataccgat cgcagcaccg attgccgcca gacccatcat cacagcggca gccatgtaca
    77161 gcagatccat attcaggttt tccatgacag tctccagttt gtttcagtta aaacgtagta
    77221 gtgttggtaa attaatgttc ttcagacgcc atcgacagat agacgatcgt cagaaccatg
    77281 aagatgaagg cttgcagcgt aatgatcagg atgtggaaaa tggcccacgg cacattcagg
    77341 atccactgtg accaccacgg caacagacca gcaatcagaa tgaaaatcag ctcaccggca
    77401 tacatgttac cgaacagtcg caaaccgagt gaaactggtt tggacagcag gcttacccct
    77461 tcaaggatta agttgacagg aatgaacgcc cagtgattga acggctgcag cgtcaactct
    77521 ttcgtgaagc cgccgatgcc tttcattttg atgctgtaga acagaatcag gataaatacg
    77581 cccagtgcca tagacagcgt tacgttcacg tccgcagacg gaaccacacg cagtgcaggc
    77641 agacccagta catgttcagc aatgtacggc agcaggtcga taggcagtaa atccatcagg
    77701 ttcatcagga atacccagac gaagatcgtc agggccagcg gagcaatcag cttgcttttg
    77761 ccatggtaca tgtctttcac gctaccatta acaaagccga tcaccagctc aatcgcggtc
    77821 tgaaacttac ctggcacacc gctggtcgcc tttttggcta cgctacggaa taaaaccagg
    77881 aacaacagac ccagcaccac cgagaagaac atggagtcaa tattgattgt ccagaaggtg
    77941 gctggggggt tttgtggatc caccagcgag aatgtacgca ggtccagctg aaggttattc
    78001 aggtggtgtc ctatgtaatc ctgcggcgtc atattttctg aagccatgat gccttttacc
    78061 ctttgttgtt aattacagcc ggtgccagta tctgaaccac cagcaccaaa acccacgtaa
    78121 cgatcagcgg caagaatacc gcctttaaaa ccgccaacgc caccaccagt aacaccaaca
    78181 tcgccagaac tttgaaagct tcgccaaatg cgaatgtcca ggccacccgg cctttcgctg
    78241 gtgtatgcgc ctggtgacgc caggcaaata tcataaacaa aacgttaggc agaaagactg
    78301 ccaggccccc gcttattgca gagacgcccc agaaggggtc tttgaggctg aacagcaatc
    78361 cacttgctat caccaccagt aactgaacga gcagaagctt ccgagcaacg tttcgactca
    78421 cgagcgacac agacatcacg tttttcactc ctgctccctt cgaggtatgc cgcgtgtcgt
    78481 ataaaacttt ctttaaggct tagagtcaag catcaaaaag cggtcaaatt atacggtgcg
    78541 cccccgtgat ttcaaacaat aagtagccaa aaggtgaata aatgtttaaa tatttttcca
    78601 gtgcatacaa ttgcgacttt tctgctaacc ctgttcgatc atgaaaaact gtaaataacg
    78661 cgtaaacact ggtgataaag cgtgcttcag atcacatatt gcgcatgttc gcgcacagca
    78721 tatttattta cttggcaaat gatgcctttg caattttatg atatttcagt ctaaaaacag
    78781 atactgtttt aataaatgac atttacacaa caaaaaccac ccattgacat ttttaataat
    78841 gttttaacag ccaatgatgg ttcttagcgc cgatttttag cagactgata ttttcactaa
    78901 tgacttattt tctgcttacc aaaaaaagcc acgttatctt gttgatgcaa aagagtgaac
    78961 gtggcgttaa atgtaaccag ttatatcagt agaaaacctg gttgttgtta acagtctaac
    79021 cggtcaattt tttatgattt ttttgataaa aattaaattt tatttgcttt aatcaccacc
    79081 agatgacgtt cgccatccag ggctggaacc tgaagtttaa ccactgattc gacctgatat
    79141 tcttcgggca acaaagcgat ttcatcttcc ggcatttgcc ctttcagcgc gtagaaacgg
    79201 ccttgctcac caggaagatg gtggcaccag ctcaccatat cgttcagaga ggcaaaagcg
    79261 cggctaatta cgccatcaaa tggcggctct gaaggaaact cttctaccct gctctgtact
    79321 ggttcaatat tctccagttt aagctcatgt tgcacctgac gaaggaaacg cacgcgttta
    79381 ccaaggctat ccaacagagt gaaatgggct tcaggacgca cgatagagag tggaatgcct
    79441 ggcagtcctg gtccggtgcc gacatcgata aaccgttcac cttgcagata cggtgccacc
    79501 acaatgctat cgagaatatg gcgtaccagc atctcattag gatcgcggac cgaagtcagg
    79561 ttgtacgctt tgttccattt atgcagcata ttcacgtagg caataagctg gtttttctgg
    79621 tgatcggtaa gcgaaatacc tgcgtctttc agcagtaagg agagtttgtt gagcacggtg
    79681 attacctgtt cttgatgcgt tgcctggtaa gcgggtgctt accaggcatt tttaatgcgt
    79741 tatgcgctac gacgcagcat accctgtttt ttcagccaca ccagcagaat ggagatggcc
    79801 gcaggcgtga cgccagaaat acgcgaagct tggccgatag aggctggttt gtgatcgtta
    79861 agtttggcga tcacttcgtt agaaagaccg gatacctggc ggtaatccag tgtcgcgggt
    79921 agcagggtgt tctcgttacg cagctgcttt tcgatctcat cttgctggcg cgcgatataa
    79981 ccttcgtatt taacctgaat ctcaacctgt tccgccgcct gttcgtctgt caacgcaggg
    80041 gcaaacggcg tcagcgtggt taatttttca taagtcattt ccggacgacg cagcagatct
    80101 tcaccactgg cttcacggga aagcggcgca gtcaggtgag cattcacttc ggctgcagct
    80161 tccgccgacg gggttaccca ggtcgatttc agacgctgac gctcacgctc gatattctca
    80221 agtttctcgt taaagcgcgc ccaacgttcg tcatccacca ggcccagttc acgaccgatt
    80281 tcagtcaaac gcagatccgc attatcttcg cgtagcatca gacgatattc tgcgcgcgaa
    80341 gtaaacatac gatacggttc tttggttcct aaagtgcaca ggtcatcaac tagtacgccg
    80401 agatacgcct gagaacgtgc cggagcccaa ccttctttgt cagcagacag acgggcagcg
    80461 ttaagaccgg ccagcaaacc ttgcgcagcg gcttcttcgt aaccggtagt gccgttaatc
    80521 tgaccagcaa agaacagccc ctggataaac ttgctctcca gcgtcggttt caggtcgcga
    80581 ggatcgaaga agtcatactc aatggcataa cccggacgca cgatcttcgc gttttccatc
    80641 ccctgcatag agcggacgat ttgcatctgc acatcgaacg gcaggctggt ggagataccg
    80701 ttcggataaa tttcattaga ggtcagtcct tccggttcaa ggaagatctg atgctgattt
    80761 ctgtcggcga agcgcatgac tttgtcttcg atcgacgggc agtagcgtgg gccgacacct
    80821 tcgatcaccc ctgcgtacat tgggctacga tcgaggttac tgcggatcac atcatgggtt
    80881 ttctcgttgg tatgagtgat ataacacggc acctgctggg gatgctggga cgcattgccc
    80941 ataaacgaga ataccggcat tgggttatcg ccatgctgtt gcgccagtac gctaaagtcg
    81001 atggttcgag catcaatacg cggtggtgtc ccggttttca gacgaccaac gcgcagcggc
    81061 agttcacgca aacggcgaga aagcggaatg gacggcggat caccagcacg gccaccgctg
    81121 taattatcca gaccgatatg aattttaccg tcgaggaacg tcccaacggt gagcacgacg
    81181 gctttggcac ggaacttcag tcccatttgg gtaacagcac cgaccacgcg atcgttttcg
    81241 acaataagat cttcaacctc ctgctggaag atcatcaggt tcggttggtt ctccagcgcc
    81301 gtacgtaccg cctgacggta gagcacacga tccgcctgag ctcgggtagc gcgaaccgcc
    81361 ggtcctttgc ttgcgtttag tatcctaaac tggatacccg cctgatcgat cgctttcgcc
    81421 atcagaccgc cgagtgcatc cacttctttt accagatgtc ccttcccaat accgccgatc
    81481 gccgggttgc agctcatctg ccccagagtg tcgatattgt gtgtcaaaag cagagtctgt
    81541 tgacccatac gcgccgcggc catcgcggcc tcggtgcctg catgaccccc gccaatgatg
    81601 atgacgtcaa aaggatccgg ataaaacatg gtgattgcct cgcataacgc ggtatgaaaa
    81661 tggattgaag cccgggccgt ggattctact caactttgtc ggcttgagaa agacctggga
    81721 tcctgggtat taaaaagaag atctatttat ttagagatct gttctattgt gatctcttat
    81781 taggatcgca ctgccctgtg gataacaagg atccggcttt taagatcaac aacctggaaa
    81841 ggatcattaa ctgtgaatga tcggtgatcc tggaccgtat aagctgggat cagaatgagg
    81901 ggttatacac aactcaaaaa ctgaacaaca gttgttcttt ggataactac cggttgatcc
    81961 aagcttcctg acagagttat ccacagtaga tcgcacgatc tgtatactta tttgagtaaa
    82021 ttaacccacg atcccagcca ttcttctgcc ggatcttccg gaatgtcgtg atcaagaatg
    82081 ttgatcttca gtgtttcgcc tgtctgtttt gcaccggaat ttttgagttc tgcctcgagt
    82141 ttatcgatag ccccacaaaa ggtgtcatat tcacgactgc caataccgat tgcgccaaag
    82201 cggactgcag aaagatcggg cttctgttcc tgcaatgctt catagaaagg agaaaggttg
    82261 tccggaatat ctccggcacc gtgggtggag ctgataacca gccagatccc tgaggcaggt
    82321 aaatcttcta acagcggacc gtgcagcgtt tcggtggtaa aacccgcctc ttccagcttt
    82381 tcagccaggt gttctgctac atattcggca ccgccgaggg tgctgccgct gataagagtg
    82441 atatctgcca taaaccgcca cctttattaa gagtggcgta ttgtacgctg tgaacgcgtt
    82501 gggatctacc tgtggaaaag tatgggatta aaaaagccga tcagggcttg atggtacgca
    82561 tgatcgggtt ttgcaggacg atcaatgtct cggtggactg aatttcatca attgtttgga
    82621 tcttgttgat aagtacatgc tggagagcgt cgatcgaacg gcacatcact tttataaaga
    82681 tgctgtagtg gccggttgtg taataggctt cagtgacttc atcaaggctt tccagctttg
    82741 ccagcgcgga agggtagtct ttggcgctct ttaatataat gccgataaag cagcctacgt
    82801 cataaccgag ctgcttcggg ctgacatcaa tacgcgcccc ggtaatgatc cccgcctgct
    82861 tcattttctc tactcgaacg tgaatcgtcc ccggactgac gccaaattgt ttcgccagtt
    82921 cggcgtaagc ggtgcgcgca ttgcccatta atgcttccag gatgccacgg tccagattgt
    82981 cgatcagata attttccata ggattttctt atgcggattg atgattcatt ctattttagc
    83041 cttctttttt aatgaatcaa aagtgagtta ggctttttat tgaatgatta ttgcatgtgt
    83101 gtcggttttt gttgcttaat cataagcaac aggacgcagg agtataaaaa atgaaaaccg
    83161 cttacattgc caaacaacgt caaattagct tcgtgaaatc tcacttttct cgtcaactgg
    83221 aagaacgtct ggggctgatc gaagtccagg cgccgattct tagccgtgtg ggggatggca
    83281 cgcaggataa cttgtcgggc tgtgaaaaag cggtgcaggt aaaagtgaaa gctctgcctg
    83341 atgcccagtt cgaagtggtt cattcactgg cgaagtggaa acgtcagacc ttagggcaac
    83401 acgacttcag cgcgggcgaa gggctgtaca cgcacatgaa agcccttcgc cccgatgaag
    83461 accgtctttc tccgttgcac tcggtctatg ttgaccagtg ggactgggaa cgcgtaatgg
    83521 gcgacggtga gcgtcaattc tcgactctga aaagcacggt agaggcgatc tgggcgggaa
    83581 ttaaagcaac cgaagctgcg gttagcgaag agtttggcct ggcaccgttc ctgccggatc
    83641 agatccactt cgtacacagc caggagttac tgtctcgtta tccggatctt gatgccaaag
    83701 ggcgtgagcg ggcgatagcg aaagatcttg gcgcggtatt ccttgtcggg attggcggca
    83761 agctgagcga tggtcatcgc cacgacgtgc gcgcaccgga ttatgatgac tggagcaccc
    83821 cgtcagagct gggccatgcg ggtctgaacg gcgatattct ggtgtggaac ccggtactgg
    83881 aagatgcgtt tgagctttcc tccatgggga tccgtgtaga tgccgacacg ctgaagcatc
    83941 aactggcgct gaccggtgac gaagatcgcc tggagctgga gtggcatcag gcgctgctgc
    84001 gcggtgaaat gccgcagacc atcggcggcg gtatcggcca gtctcgtttg actatgctgc
    84061 tgctgcaact gccgcatatc ggccaggttc agtgtggagt atggccagct gctgttcgcg
    84121 agagcgtccc ttctctgctg taataattta tcgccgccag cgtctgagca ggcggcttcg
    84181 catcccggta tcaaagcgcc agatatgatc gaaaatgcgc atgatgccgg gtttgccgtg
    84241 tgccgacatc gccacggcat gaaagcgatg ctgatgtacc cgctgcagct ctttcacttt
    84301 actcgtcacg tcgtcaggca accgctgagc gataaaatca gaaatcacca ccgcatcggc
    84361 atcaaaccat tccctgcttt gcaagcgttc cataatggcg cgaaaacaac tggcaagatc
    84421 ggtgccgcca cgaaactgct ggcttaaaaa acggattgct tgttcgatgc cttgtgggcc
    84481 tgaaagctca taacggacga tctcggtgga aaatagcata atatagcagc gccggttttc
    84541 tgcgagagca atgcgcatca aggccaggca gaacgctttc gcacactgtt cattaaagcc
    84601 gcccattgag ccggaagtat ccacacagac aataaacggc ccgcgcggct gttcatcgta
    84661 atctttatgt accaccggac gttcgatcac tttttcacgc cacgactcac cgtgcaggcg
    84721 ataggtgagc aactgttttt ccaccagccg acggtaaaac tcatactcca gttccgttat
    84781 ccctagtgtc gccagttctg gcggcaggag acgtaaaata tcatcgcttt gttgcagacc
    84841 atcaacctgc tcaggaaccg tcgccggttc gcgcaccatg gtgcggaagg tttccatctg
    84901 cgcatcgttg cgcggtattg atttggcttc ccgagaacgc cccagctgct ctgccaggcg
    84961 tttcagttcc ggctgttcgt taagaaattc accgtatttc acaatcaact gatagtcgcc
    85021 acgtttaagc tgaccggcgc tcatatccca cagacgacca gctgcagtat tgttatctgc
    85081 gagaatcggt tcaagttgtc cgctcagcgt catgcgttcc tgaacttcac tcaacagttg
    85141 ttcgcgttct tcttctaata gctgttgatt taacgtcgtt gcttgcacga tcagacttaa
    85201 acgccaacgc tggagaaaaa gcgtgtgtaa cgctgaagtg atcgtgctgt tagcatcaac
    85261 caactgtcgg gcttgttctg cccatggaga attcagacga tgcagtaagt ccaggatctg
    85321 tggtagctgc acaataaact gtggcgtgga gaggagctgg ctttgctgat agcacatcac
    85381 ctcttcggtg agttccggcg ggactcgggc atctttcagc cgactgcgca gcgcttcacg
    85441 ccagcgggga acatcatcag tgattgccgc cttcaggcgt gggaattttt caaagaagac
    85501 cgccagctgc ggtgaggcca gcagtgcgat gatcatctct tcgatcaatc cctcttcgct
    85561 gacggccagc atcacattaa gcgtatccag cgttagcatt gttgtgcctg gcgaatctgt
    85621 gcaccgacat cctgtaggct ggcttcaatg cgacctaacc agtcgccagg gataaacaga
    85681 catttttgct gttcgctgaa taaagcgtgt tgcttgcgcc agtcgctttc aagctcttcc
    85741 agttgttgtt ttatttcacc aggcagacct tcagccgatg aaccgggaag tgccagcgta
    85801 ctgccttgta aacttacatc gcgaacgaca agatgttggg cgctatcaac ttccagattc
    85861 agtttctggg caaagccgat accgttcagt ttaccgcgaa tttcaccacc tttgctcagc
    85921 cactgttcca gcgcgctacg ctcaaaggag atatgaacca cttccatatc atgcagtttt
    85981 aacggttttt gcagcagtag agtcagagtg gaagcagtaa cgttaacagg aagttgatac
    86041 tgctgacgac ggctgaaaat gccgcccaga cgaattaccg ttaaggctgt tttatcgctt
    86101 tgttgctgct ggagttgcag gtgacgttgc acaatcgcgc caaggcgagt caacatccct
    86161 tgctgttgcc aggcgtgacc ggtcatcaat acatcaattt gttgttgtat caaattcagg
    86221 ctttgcgcgt cataccacag gcaatctttc agcaaaatga gatctaccgg ggcaacagca
    86281 ctgcgaccgc taaaaaaggc gctggcctgc aataatcgga tcgctttttt ccagcgacga
    86341 tccgagacat aaggcgcatc cggtaattta tccagttgct ggcgcagcat aaaaatcagc
    86401 tcaaatacat gatcgggcag cgtaatttca ccaatctctt tctgccagcg ttcatattct
    86461 tcatctgtga cctgcaaggc atcaggaacc ggattgtcgt tttcatcctg ttgactggtc
    86521 agcatggagc ggaaattcgc tttatcctgc actttatcta accacagacg aatcagcatg
    86581 cggtcatata acgcttccag actgctgtct gcttccggca gctcgttgga ggccgccacc
    86641 agcagacgca tcgggatttt ttctacgtgt gcaccgttgc ggaactggcg ctcgttaatg
    86701 gcggtgagca aggtattaag aattgccggg cccgctttcc agatctcatc cagaaagaca
    86761 atttctgctt ccggcaggta accgctggtt aaacgttcat agcgcccttc atcttttagc
    86821 gcctgaatag aaaggggacc aaaaacttct tccggcgtgg agaagcgggt catcagatat
    86881 tcaaacgcgc gggcattctg aaaggcgaat tttaagcgcc gggcgatcaa acttttggca
    86941 atacctggcg ggccaaggag gaacacactt tcaccactta atgccgctaa taaacacaag
    87001 cggatggcgt ggctacgttc ataaagcccc ttttccaacg aactgctcag gcgggaaatt
    87061 ctttccgcta ataaatgagg gtgagccata atgaagtggc gtcctttcgt caaaagttct
    87121 gcgtaaattg cgagtataga cgtttcttgc tggtggctaa aatagtctca aagggggggt
    87181 atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg ccgttaatcg
    87241 tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat atttcaacga
    87301 aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc ctcgcggcga
    87361 ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt gaatgtttgt
    87421 ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta tcgctgatct
    87481 tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg cgggcagata
    87541 acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc
    87601 gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc tatggtgaag
    87661 tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa atcgtcgccc
    87721 cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta ttatttatga
    87781 ttcaaaaaca tggcacggct atggtcggta agctgtttgc gccgatcatg ctgacctggt
    87841 ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa gtgctgcatg
    87901 cgctgaatcc aatgtgggcg gtgcatttct tccttgaata caaaacggtt tcttttattg
    87961 cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct gatatggggc
    88021 actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct tccttgaccc
    88081 ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt aagaacccgt
    88141 tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc gcactggcga
    88201 cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt caggcggtac
    88261 gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag tcagggcaaa
    88321 tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg attgtcagct
    88381 ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga accatggtgc
    88441 tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat aagtattttg
    88501 ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc gctaacctcg
    88561 ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg tttatcgtga
    88621 tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa catggtaact
    88681 ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg cccgggaccg
    88741 cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat aaccttaaac
    88801 ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa gacgctccat
    88861 atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc tggcgcgtgg
    88921 tggcaagtta tggttggcga gaaacgccaa acgtagaaga agttttccac cgctgcggtc
    88981 tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat gagtcgttga
    89041 tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac
    89101 gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt atcgaactgg
    89161 gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg cgtttttgtt
    89221 ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt aacgtcacga
    89281 tggttttccc aactcagtca ggattaaact gtgagtcagc gaaacgtttc gctgatggag
    89341 aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat ctcccgtctg
    89401 ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa aagtacaacg
    89461 cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct gggcgtcgtc
    89521 acaaatgaaa tgcaggtcga ggcggccatt atcgcggaag agatcaaaca ccataatccg
    89581 caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca gggaaatacc
    89641 attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg
    89701 gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg
    89761 ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag ccttcccggg
    89821 cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgcgtga tggcgctggt
    89881 gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggca tctatactcg
    89941 cgatgccggt acgcttttat ggctggggaa agaaacgaca tttaccgggc caaaatcttc
    90001 ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc agttgaccat
    90061 tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa
    90121 aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
    90181 caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
    90241 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
    90301 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg gtattgtcta
    90361 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
    90421 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
    90481 gatggtgggt cgcaagctgg aagatcaata tccgcacctg gacaaagcgc cgggagatat
    90541 ccgcctgaaa gtcgataatc tctacggacc tggcgttaac gatgtctctt ttactttacg
    90601 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
    90661 agtgctctac ggcgcactac cgcgcaccag cggttacgtc accctggatg ggcatgaagt
    90721 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
    90781 taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
    90841 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
    90901 tgatttcatt cgtctgttta atgtgaaaac tccgtcgatg gaacaggcaa ttggtctgct
    90961 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac gccccaaagt
    91021 gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
    91081 actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat cggagatgcc
    91141 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
    91201 atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg gcaagcttaa
    91261 tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc cgttatttca
    91321 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
    91381 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
    91441 cctcagtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatcg
    91501 acttatcggt aggttctctg ttggcgctga ccggcgcagt tgctgcatct atcgtcggta
    91561 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc gcaattggtg
    91621 cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta
    91681 tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca gtgaataccg
    91741 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
    91801 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
    91861 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
    91921 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
    91981 tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg gggactggct
    92041 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
    92101 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
    92161 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
    92221 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
    92281 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
    92341 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
    92401 cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg
    92461 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
    92521 caaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct gtgaagatgg
    92581 ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
    92641 tgagccacat tgcttctgat aacgtactgg gcggcaaaat cgctggtgat tacatcgcga
    92701 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt acatccgcag
    92761 cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt aatgttcttg
    92821 ccagccagcc agcagatttt gatcgcacta aaggtttgaa cgtaatgcag aacctgttga
    92881 ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
    92941 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
    93001 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
    93061 atcagattgg cgcgaaaggc gtcgaaaccg cagataaagt gctgaaaggc gagaaagttc
    93121 aggctaagta tccggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
    93181 tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca
    93241 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
    93301 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
    93361 tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
    93421 cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac
    93481 gggtgatgac agcattggtg agagcgttcg ccagcagctc gccactgata acattgatat
    93541 tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
    93601 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgcccttt ccccggcgct
    93661 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagca ttattaatgc agctggaatc
    93721 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
    93781 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat
    93841 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
    93901 tgcagcgaag gcggcgcagg tactgcatga aaaaggtatc cgtactgtac tgattacttt
    93961 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
    94021 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggtgcgt taatcacggc
    94081 attgctggaa gaaaaaccat tgccagaggc gattcgtttt gcccatgctg ccgctgcgat
    94141 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
    94201 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgcc tggcgggcgt
    94261 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
    94321 cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagctc tggcgcgtag
    94381 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
    94441 ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt
    94501 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
    94561 aaaacgcgtt gatggcttgc tgttactgtg caccgaaacg catcaacctt cgcgtgaaat
    94621 catgcaacgt tatccgacag tgcctactgt gatgatggac tgggctccgt tcgatggcga
    94681 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
    94741 cgataaaggt catacccgta tcgcctgtat taccggcccg ctggataaaa ctccggcgcg
    94801 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
    94861 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgctatgc gccaactgct
    94921 atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
    94981 ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta
    95041 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
    95101 agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct
    95161 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg cttagattta
    95221 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
    95281 gaagcaacag taataatgcc cattgtgata aacgtatagt ggaattgttc gacagtcgtt
    95341 gtgccttcca ttccttcata aacgcgaagg acggccgcac ttacagcaac gcctaaacta
    95401 atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcatt gtcatcggtc
    95461 agatcggcaa gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc catccctaat
    95521 ataaacaacg gcaagatcag catccatata gccattgctg gtgattgcaa agagaactga
    95581 gcgatcatta gcccaataat caccgtgatc cccactaacg tatggcgata gcccagacga
    95641 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
    95701 atacagccag caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
    95761 aacggtacac agccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa
    95821 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtgcgtcg tgcatggaga
    95881 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg
    95941 acaatctttt ccccgaatag ctctattccg cttgagaaga gaacaaggct gaggccaaac
    96001 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
    96061 cgcgcgtaaa gaaggcccgc aatacctatg gggatattga ttaaaaatat ccagtgccag
    96121 gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc caccagaccc
    96181 ggcatggcga caaaattcaa tactggaaga agttcattac gaggataagc gcgcagtaag
    96241 gccagccgag caacaggcat catcattgcg ccgcctatcc cctgaataac ccggaagaca
    96301 accagctgtg gtagcgaatt agaaagtgcg caggccagag aacccaatgt gaacagactc
    96361 acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
    96421 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
    96481 cgattaaggc tatgagcgat tgcgggtaag gcggtattaa gaatagtggc atcaagtgcc
    96541 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg ccatactgcg cttcttttta
    96601 tcgctcattc aatgtcctgt tatcgggtta tcacttatca ggtgagcgta gcagcgcctg
    96661 acaagcttta aatgccgcgt cgccatcgct ttggataatc gcatcgacaa tcgcctgatg
    96721 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
    96781 atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
    96841 atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc
    96901 catttcggcc attaacgtat tgagatgcgc tttctgttcc gcggtgccaa ccgttgctgc
    96961 cagtaggcag gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
    97021 atgaaagttc tcttctgtca tccaccaggt aagcaattcc tgatcaagaa aattccagtt
    97081 cgattgtggc atgacccgag taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
    97141 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
    97201 aatctcaccg ggcaaaatgg tgccgggttc atattcacct tttaagatcc gttgcgccag
    97261 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
    97321 cattacttat cttccttttt ctttttattc ctccttagta tgccactagg aagtgtgatt
    97381 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggct tttgctgaaa
//
LOCUS       DBBGLQ010000005        96521 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.005, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000005 DBBGLQ010000000
VERSION     DBBGLQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.005"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..427
                     /locus_tag="ACIYSB_000482"
     CDS             <1..427
                     /locus_tag="ACIYSB_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5427602.1"
                     /translation="YNAEQFRNIGERSPKIPFTIPLVNCPMTGWEHDNGNVEASFRLW
                     LETRDNGTVPNFPNLAKVGSFAGIAATGVGIRIDDAESGNIMPLNAMGNDNTVYQIPA
                     ESNGIVNVDLIAYYVSTVVPSEITPGEADAIVNVTLDYR"
     gene            507..1202
                     /gene="yraI"
                     /locus_tag="ACIYSB_000483"
     CDS             507..1202
                     /gene="yraI"
                     /locus_tag="ACIYSB_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGC5427603.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLR"
     gene            1231..3747
                     /gene="yraJ"
                     /locus_tag="ACIYSB_000484"
     CDS             1231..3747
                     /gene="yraJ"
                     /locus_tag="ACIYSB_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGC5427604.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQQLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVSAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGVGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3758..4849
                     /gene="yraK"
                     /locus_tag="ACIYSB_000485"
     CDS             3758..4849
                     /gene="yraK"
                     /locus_tag="ACIYSB_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5427605.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKNNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(4892..5752)
                     /gene="rsmI"
                     /locus_tag="ACIYSB_000486"
     CDS             complement(4892..5752)
                     /gene="rsmI"
                     /locus_tag="ACIYSB_000486"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGC5427606.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5817..7853
                     /gene="lpoA"
                     /locus_tag="ACIYSB_000487"
     CDS             5817..7853
                     /gene="lpoA"
                     /locus_tag="ACIYSB_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGC5427607.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDAQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITLRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRNLSWLQYQQGQVVPVS"
     gene            7811..8206
                     /gene="yraN"
                     /locus_tag="ACIYSB_000488"
     CDS             7811..8206
                     /gene="yraN"
                     /locus_tag="ACIYSB_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGC5427608.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8226..8816
                     /gene="diaA"
                     /locus_tag="ACIYSB_000489"
     CDS             8226..8816
                     /gene="diaA"
                     /locus_tag="ACIYSB_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGC5427609.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8826..9401
                     /gene="dolP"
                     /locus_tag="ACIYSB_000490"
     CDS             8826..9401
                     /gene="dolP"
                     /locus_tag="ACIYSB_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGC5427610.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9515..10555)
                     /gene="yraQ"
                     /locus_tag="ACIYSB_000491"
     CDS             complement(9515..10555)
                     /gene="yraQ"
                     /locus_tag="ACIYSB_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGC5427611.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMAAV
                     SGVIVGGLALLF"
     gene            complement(10628..11263)
                     /gene="yraR"
                     /locus_tag="ACIYSB_000492"
     CDS             complement(10628..11263)
                     /gene="yraR"
                     /locus_tag="ACIYSB_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGC5427612.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11391..11909
                     /gene="yhbO"
                     /locus_tag="ACIYSB_000493"
     CDS             11391..11909
                     /gene="yhbO"
                     /locus_tag="ACIYSB_000493"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGC5427613.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(11889..12332)
                     /gene="yhbP"
                     /locus_tag="ACIYSB_000494"
     CDS             complement(11889..12332)
                     /gene="yhbP"
                     /locus_tag="ACIYSB_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGC5427614.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12383..12685
                     /gene="yhbQ"
                     /locus_tag="ACIYSB_000495"
     CDS             12383..12685
                     /gene="yhbQ"
                     /locus_tag="ACIYSB_000495"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGC5427615.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLVFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12672..13175)
                     /gene="yhbS"
                     /locus_tag="ACIYSB_000496"
     CDS             complement(12672..13175)
                     /gene="yhbS"
                     /locus_tag="ACIYSB_000496"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGC5427616.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13169..13693)
                     /gene="ubiT"
                     /locus_tag="ACIYSB_000497"
     CDS             complement(13169..13693)
                     /gene="ubiT"
                     /locus_tag="ACIYSB_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGC5427617.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13902..14897
                     /gene="ubiU"
                     /locus_tag="ACIYSB_000498"
     CDS             13902..14897
                     /gene="ubiU"
                     /locus_tag="ACIYSB_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGC5427618.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTIGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14906..15784
                     /gene="ubiV"
                     /locus_tag="ACIYSB_000499"
     CDS             14906..15784
                     /gene="ubiV"
                     /locus_tag="ACIYSB_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGC5427619.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            15865..16872
                     /gene="yhbW"
                     /locus_tag="ACIYSB_000500"
     CDS             15865..16872
                     /gene="yhbW"
                     /locus_tag="ACIYSB_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HGC5427620.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16990..18234)
                     /gene="mtr"
                     /locus_tag="ACIYSB_000501"
     CDS             complement(16990..18234)
                     /gene="mtr"
                     /locus_tag="ACIYSB_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGC5427621.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18388..20277)
                     /gene="deaD"
                     /locus_tag="ACIYSB_000502"
     CDS             complement(18388..20277)
                     /gene="deaD"
                     /locus_tag="ACIYSB_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGC5427622.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPCDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20270..20350)
                     /gene="yrbN"
                     /locus_tag="ACIYSB_000503"
     CDS             complement(20270..20350)
                     /gene="yrbN"
                     /locus_tag="ACIYSB_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGC5427623.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20457..21341)
                     /gene="nlpI"
                     /locus_tag="ACIYSB_000504"
     CDS             complement(20457..21341)
                     /gene="nlpI"
                     /locus_tag="ACIYSB_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGC5427624.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21450..23585)
                     /gene="pnp"
                     /locus_tag="ACIYSB_000505"
     CDS             complement(21450..23585)
                     /gene="pnp"
                     /locus_tag="ACIYSB_000505"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGC5427625.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAQLLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23832..24101)
                     /gene="rpsO"
                     /locus_tag="ACIYSB_000506"
     CDS             complement(23832..24101)
                     /gene="rpsO"
                     /locus_tag="ACIYSB_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGC5427626.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24250..25194)
                     /gene="truB"
                     /locus_tag="ACIYSB_000507"
     CDS             complement(24250..25194)
                     /gene="truB"
                     /locus_tag="ACIYSB_000507"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGC5427627.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25194..25595)
                     /gene="rbfA"
                     /locus_tag="ACIYSB_000508"
     CDS             complement(25194..25595)
                     /gene="rbfA"
                     /locus_tag="ACIYSB_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGC5427628.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25759..28431)
                     /gene="infB"
                     /locus_tag="ACIYSB_000509"
     CDS             complement(25759..28431)
                     /gene="infB"
                     /locus_tag="ACIYSB_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGC5427629.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28456..29943)
                     /gene="nusA"
                     /locus_tag="ACIYSB_000510"
     CDS             complement(28456..29943)
                     /gene="nusA"
                     /locus_tag="ACIYSB_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGC5427630.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29971..30423)
                     /gene="rimP"
                     /locus_tag="ACIYSB_000511"
     CDS             complement(29971..30423)
                     /gene="rimP"
                     /locus_tag="ACIYSB_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGC5427631.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30630..30706)
                     /locus_tag="ACIYSB_000512"
     tRNA            complement(30630..30706)
                     /locus_tag="ACIYSB_000512"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30670..30672),aa:Met,seq:cat)
     gene            31054..32397
                     /gene="argG"
                     /locus_tag="ACIYSB_000513"
     CDS             31054..32397
                     /gene="argG"
                     /locus_tag="ACIYSB_000513"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGC5427632.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(32405..34030)
                     /gene="yhbX"
                     /locus_tag="ACIYSB_000514"
     CDS             complement(32405..34030)
                     /gene="yhbX"
                     /locus_tag="ACIYSB_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGC5427633.1"
                     /translation="MTVFNKFARTFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34489..34575)
                     /locus_tag="ACIYSB_000515"
     tRNA            complement(34489..34575)
                     /locus_tag="ACIYSB_000515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34539..34541),aa:Leu,seq:gag)
     gene            complement(34590..34922)
                     /gene="secG"
                     /locus_tag="ACIYSB_000516"
     CDS             complement(34590..34922)
                     /gene="secG"
                     /locus_tag="ACIYSB_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGC5427634.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35150..36487)
                     /gene="glmM"
                     /locus_tag="ACIYSB_000517"
     CDS             complement(35150..36487)
                     /gene="glmM"
                     /locus_tag="ACIYSB_000517"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGC5427635.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36480..37328)
                     /gene="folP"
                     /locus_tag="ACIYSB_000518"
     CDS             complement(36480..37328)
                     /gene="folP"
                     /locus_tag="ACIYSB_000518"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGC5427636.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37418..39352)
                     /gene="ftsH"
                     /locus_tag="ACIYSB_000519"
     CDS             complement(37418..39352)
                     /gene="ftsH"
                     /locus_tag="ACIYSB_000519"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGC5427637.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39452..40081)
                     /gene="rlmE"
                     /locus_tag="ACIYSB_000520"
     CDS             complement(39452..40081)
                     /gene="rlmE"
                     /locus_tag="ACIYSB_000520"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGC5427638.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40207..40500
                     /gene="yhbY"
                     /locus_tag="ACIYSB_000521"
     CDS             40207..40500
                     /gene="yhbY"
                     /locus_tag="ACIYSB_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGC5427639.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40656..41132)
                     /gene="greA"
                     /locus_tag="ACIYSB_000522"
     CDS             complement(40656..41132)
                     /gene="greA"
                     /locus_tag="ACIYSB_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGC5427640.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41380..42813
                     /gene="dacB"
                     /locus_tag="ACIYSB_000523"
     CDS             41380..42813
                     /gene="dacB"
                     /locus_tag="ACIYSB_000523"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGC5427641.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            42868..42975
                     /locus_tag="ACIYSB_000524"
                     /pseudo
     CDS             42868..42975
                     /locus_tag="ACIYSB_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(42999..44171)
                     /gene="cgtA"
                     /locus_tag="ACIYSB_000525"
     CDS             complement(42999..44171)
                     /gene="cgtA"
                     /locus_tag="ACIYSB_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGC5427642.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDNWDEDDEEGVEFIYKR"
     gene            complement(44187..45152)
                     /gene="yhbE"
                     /locus_tag="ACIYSB_000526"
     CDS             complement(44187..45152)
                     /gene="yhbE"
                     /locus_tag="ACIYSB_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGC5427643.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45279..45536)
                     /gene="rpmA"
                     /locus_tag="ACIYSB_000527"
     CDS             complement(45279..45536)
                     /gene="rpmA"
                     /locus_tag="ACIYSB_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGC5427644.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45557..45868)
                     /gene="rplU"
                     /locus_tag="ACIYSB_000528"
     CDS             complement(45557..45868)
                     /gene="rplU"
                     /locus_tag="ACIYSB_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGC5427645.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46127..47098
                     /gene="ispB"
                     /locus_tag="ACIYSB_000529"
     CDS             46127..47098
                     /gene="ispB"
                     /locus_tag="ACIYSB_000529"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGC5427646.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47327..47605
                     /gene="sfsB"
                     /locus_tag="ACIYSB_000530"
     CDS             47327..47605
                     /gene="sfsB"
                     /locus_tag="ACIYSB_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGC5427647.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47653..48912)
                     /gene="murA"
                     /locus_tag="ACIYSB_000531"
     CDS             complement(47653..48912)
                     /gene="murA"
                     /locus_tag="ACIYSB_000531"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGC5427648.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48967..49221)
                     /gene="ibaG"
                     /locus_tag="ACIYSB_000532"
     CDS             complement(48967..49221)
                     /gene="ibaG"
                     /locus_tag="ACIYSB_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGC5427649.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49381..49674)
                     /gene="mlaB"
                     /locus_tag="ACIYSB_000533"
     CDS             complement(49381..49674)
                     /gene="mlaB"
                     /locus_tag="ACIYSB_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGC5427650.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49674..50309)
                     /gene="mlaC"
                     /locus_tag="ACIYSB_000534"
     CDS             complement(49674..50309)
                     /gene="mlaC"
                     /locus_tag="ACIYSB_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGC5427651.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50328..50879)
                     /gene="mlaD"
                     /locus_tag="ACIYSB_000535"
     CDS             complement(50328..50879)
                     /gene="mlaD"
                     /locus_tag="ACIYSB_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGC5427652.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50884..51666)
                     /gene="mlaE"
                     /locus_tag="ACIYSB_000536"
     CDS             complement(50884..51666)
                     /gene="mlaE"
                     /locus_tag="ACIYSB_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGC5427653.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51674..52483)
                     /gene="mlaF"
                     /locus_tag="ACIYSB_000537"
     CDS             complement(51674..52483)
                     /gene="mlaF"
                     /locus_tag="ACIYSB_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGC5427654.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52693..53670
                     /gene="yrbG"
                     /locus_tag="ACIYSB_000538"
     CDS             52693..53670
                     /gene="yrbG"
                     /locus_tag="ACIYSB_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGC5427655.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLVVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53684..54670
                     /gene="kdsD"
                     /locus_tag="ACIYSB_000539"
     CDS             53684..54670
                     /gene="kdsD"
                     /locus_tag="ACIYSB_000539"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGC5427656.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54691..55257
                     /gene="kdsC"
                     /locus_tag="ACIYSB_000540"
     CDS             54691..55257
                     /gene="kdsC"
                     /locus_tag="ACIYSB_000540"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGC5427657.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55254..55829
                     /gene="lptC"
                     /locus_tag="ACIYSB_000541"
     CDS             55254..55829
                     /gene="lptC"
                     /locus_tag="ACIYSB_000541"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGC5427658.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55798..56355
                     /gene="lptA"
                     /locus_tag="ACIYSB_000542"
     CDS             55798..56355
                     /gene="lptA"
                     /locus_tag="ACIYSB_000542"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGC5427659.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56362..57087
                     /gene="lptB"
                     /locus_tag="ACIYSB_000543"
     CDS             56362..57087
                     /gene="lptB"
                     /locus_tag="ACIYSB_000543"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGC5427660.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57135..58568
                     /gene="rpoN"
                     /locus_tag="ACIYSB_000544"
     CDS             57135..58568
                     /gene="rpoN"
                     /locus_tag="ACIYSB_000544"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGC5427661.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58591..58878
                     /gene="hpf"
                     /locus_tag="ACIYSB_000545"
     CDS             58591..58878
                     /gene="hpf"
                     /locus_tag="ACIYSB_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGC5427662.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58996..59487
                     /gene="ptsN"
                     /locus_tag="ACIYSB_000546"
     CDS             58996..59487
                     /gene="ptsN"
                     /locus_tag="ACIYSB_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGC5427663.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59533..60387
                     /gene="rapZ"
                     /locus_tag="ACIYSB_000547"
     CDS             59533..60387
                     /gene="rapZ"
                     /locus_tag="ACIYSB_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGC5427664.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60384..60656
                     /gene="npr"
                     /locus_tag="ACIYSB_000548"
     CDS             60384..60656
                     /gene="npr"
                     /locus_tag="ACIYSB_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGC5427665.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60870..61502
                     /gene="yrbL"
                     /locus_tag="ACIYSB_000549"
     CDS             60870..61502
                     /gene="yrbL"
                     /locus_tag="ACIYSB_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGC5427666.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61499..62227)
                     /gene="mtgA"
                     /locus_tag="ACIYSB_000550"
     CDS             complement(61499..62227)
                     /gene="mtgA"
                     /locus_tag="ACIYSB_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGC5427667.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62224..62877)
                     /gene="elbB"
                     /locus_tag="ACIYSB_000551"
     CDS             complement(62224..62877)
                     /gene="elbB"
                     /locus_tag="ACIYSB_000551"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGC5427668.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63107..65443)
                     /gene="arcB"
                     /locus_tag="ACIYSB_000552"
     CDS             complement(63107..65443)
                     /gene="arcB"
                     /locus_tag="ACIYSB_000552"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGC5427669.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65539..66468)
                     /gene="yhcC"
                     /locus_tag="ACIYSB_000553"
     CDS             complement(65539..66468)
                     /gene="yhcC"
                     /locus_tag="ACIYSB_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGC5427670.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            67143..71603
                     /gene="gltB"
                     /locus_tag="ACIYSB_000554"
     CDS             67143..71603
                     /gene="gltB"
                     /locus_tag="ACIYSB_000554"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGC5427671.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPTDLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71616..73034
                     /gene="gltD"
                     /locus_tag="ACIYSB_000555"
     CDS             71616..73034
                     /gene="gltD"
                     /locus_tag="ACIYSB_000555"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGC5427672.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73594..74358
                     /gene="gltF"
                     /locus_tag="ACIYSB_000556"
     CDS             73594..74358
                     /gene="gltF"
                     /locus_tag="ACIYSB_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1120 domain-containing protein"
                     /protein_id="HGC5427673.1"
                     /translation="MFFKKNLTTAAICAALSVAAFSAMATDSTDTELTIIGEYTPGAC
                     TPVVTGGGIVDYGKHHNSALNPTGKSNKLVQLGRKNSTLNITCTAPTLIAVTSKDNRQ
                     STIVALNDTSYIEKAYDTLVDMKGTKNAFGLGSAPNGQKIGAASIGIDRSNGGIHAAD
                     DTGEIPVDLIQTDHWSAATPTWKASSNGAFCSLTSCSAIERGYSVAKTGELTPVAITA
                     VTFPLLIDAAVNDNTILGSDETIKLDGNVTISVQYL"
     gene            74530..75204
                     /gene="yhcA"
                     /locus_tag="ACIYSB_000557"
     CDS             74530..75204
                     /gene="yhcA"
                     /locus_tag="ACIYSB_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus chaperone family protein"
                     /protein_id="HGC5427674.1"
                     /translation="MLRHITFTVFITTSMNTLATGMVPETSVLLVDEKRGEASINIKN
                     TDDHPSLLYTTIVDLPESNKSIRLIPTQPVIRVEAGQVQQVRFLLQATVPLQSEELKR
                     VTFEGIPPKDDKSSRVTVSIRQDLPVLIHPASLPEERETWKFLEWRKNGDQIEISNPS
                     NYVVRMTLQFKTLPSGKTGAINKTYFLPHTSTTTALTNATDTKVEFYPASRYGYRGNK
                     YVTDLK"
     gene            75225..77606
                     /gene="yhcD"
                     /locus_tag="ACIYSB_000558"
     CDS             75225..77606
                     /gene="yhcD"
                     /locus_tag="ACIYSB_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGC5427675.1"
                     /translation="MLKKTLLAYTIGFAFSPPANADGIEIAAVDFDRETLKSLGVDPN
                     ISHYFSRSARFLPGEYSLIVSVNGEKKGNIATRFDENGDICLDQAFLQQAGLKIPSEE
                     KNGCYDYILSYPGTTITPLPNQEALDIIVSPQAIIPIGLDLTNAATGGTAALLNYSLM
                     SSRAEFSNGSSDYSQAALEGGININDWMLRSHQFLTQTNGTFSNQNSSTYLQRTFTDL
                     KTLMRAGEVNLNNSVLEGASIYGIEIAPDNALQTSGSGVQVTGIANTSQARVEIRQQG
                     VLIHSILVPAGAFTIPDVPVRNGNSDLNVTVVETDGSSHNYIVPSTLFNQHVESFQGY
                     RFAIGRVDDDYDESPWVISASSGWNLTRWSAMNGGVIVAENYQAASIRSSLVPLPDLT
                     VSSQISTSQDTKDSLQGQKYRLDANYNLPFSLGLTTSLTRSDRHYRELSEAIDDDYTD
                     PTKSTYALGLNWSNSILGGFNISGYKTYSYDGDNDSSNLNINWNKAFKHATVSVNWQH
                     QLSASENNEDDGDLFYVNISIPFGRSNTATLYTRHDDHKTHYGTGVMGVVSDEMSYYV
                     NAERDHDERETSLNGSISSNLHYTQVSLAAGASGSDSRTYNGTMSGGIAVHDQGVTFS
                     PWTINDTFAIAKMDNNIAGVRITSQAGPVWTDFRGNAVIPSIQPWRTSGVEIDTASLP
                     KNVDIGNGTKMIKQGRGAVGKVGFSAITQRRALLNITLSDGKKLPRGVAIEDSEGNYL
                     TTSVDDGVVFLNNIKPDMVLDIKDEQQSCRIHLTFPEDAPKDVFYETATGECQ"
     gene            77603..78148
                     /locus_tag="ACIYSB_000559"
     CDS             77603..78148
                     /locus_tag="ACIYSB_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcE family protein"
                     /protein_id="HGC5427676.1"
                     /translation="MKRIITGCLLLNFAMAAQAECNISSSIQNIDYGKRSAAMRQVDR
                     GKTTQLADRTITLVMQCDQDAHIRVQLNTANISNNGFGFGPNGSLNLIASDAFSGSNN
                     LDLALASGKNDNPGSTGTASISTSPNNWLVFMQNGQEVVIDSGKSVSLTLTMAPAFKD
                     EGELTDMTDITGNLTVLVEAK"
     gene            78145..78861
                     /gene="yhcF"
                     /locus_tag="ACIYSB_000560"
     CDS             78145..78861
                     /gene="yhcF"
                     /locus_tag="ACIYSB_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1120 domain-containing protein"
                     /protein_id="HGC5427677.1"
                     /translation="MNNVKLLIAGSAFFAMSAQAADRVSIDVKVTLEAAACTPILSNG
                     GVVNFGSHSVNRLSTQHYTQIGTRNINMTITCESATGIAITARDTRMDSMTTGKDSGG
                     QSGVKYTLNGGGYISQTTRLFGLGKTKDNKNIGSYAVLIDSNNISASNGSQTLAVSIA
                     GADAVITGQKRAWQTLTAYPLAVDQSYYYTFVKPGETTPTPVTNAIIPLQVSASIAND
                     LGGSEKIELDGKAVISVVYL"
     gene            79046..80173
                     /gene="yhcG"
                     /locus_tag="ACIYSB_000561"
     CDS             79046..80173
                     /gene="yhcG"
                     /locus_tag="ACIYSB_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HGC5427678.1"
                     /translation="MESLSEGTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYQE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLCLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTMSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPRSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(80233..80697)
                     /gene="nanQ"
                     /locus_tag="ACIYSB_000562"
     CDS             complement(80233..80697)
                     /gene="nanQ"
                     /locus_tag="ACIYSB_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGC5427679.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(80694..81569)
                     /gene="nanK"
                     /locus_tag="ACIYSB_000563"
     CDS             complement(80694..81569)
                     /gene="nanK"
                     /locus_tag="ACIYSB_000563"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGC5427680.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEFQALDGDITDMVFITVSTGVGGGVVSGCKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(81566..82255)
                     /gene="nanE"
                     /locus_tag="ACIYSB_000564"
     CDS             complement(81566..82255)
                     /gene="nanE"
                     /locus_tag="ACIYSB_000564"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGC5427681.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(82303..83793)
                     /gene="nanT"
                     /locus_tag="ACIYSB_000565"
     CDS             complement(82303..83793)
                     /gene="nanT"
                     /locus_tag="ACIYSB_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGC5427682.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(83902..84795)
                     /gene="nanA"
                     /locus_tag="ACIYSB_000566"
     CDS             complement(83902..84795)
                     /gene="nanA"
                     /locus_tag="ACIYSB_000566"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGC5427683.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(84917..85708)
                     /gene="nanR"
                     /locus_tag="ACIYSB_000567"
     CDS             complement(84917..85708)
                     /gene="nanR"
                     /locus_tag="ACIYSB_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGC5427684.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            86087..87454
                     /gene="dcuC"
                     /locus_tag="ACIYSB_000568"
     CDS             86087..87454
                     /gene="dcuC"
                     /locus_tag="ACIYSB_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGC5427685.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(87497..87994)
                     /gene="sspB"
                     /locus_tag="ACIYSB_000569"
     CDS             complement(87497..87994)
                     /gene="sspB"
                     /locus_tag="ACIYSB_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGC5427686.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(88000..88638)
                     /gene="sspA"
                     /locus_tag="ACIYSB_000570"
     CDS             complement(88000..88638)
                     /gene="sspA"
                     /locus_tag="ACIYSB_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGC5427687.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(89033..89425)
                     /gene="rpsI"
                     /locus_tag="ACIYSB_000571"
     CDS             complement(89033..89425)
                     /gene="rpsI"
                     /locus_tag="ACIYSB_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGC5427688.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(89441..89869)
                     /gene="rplM"
                     /locus_tag="ACIYSB_000572"
     CDS             complement(89441..89869)
                     /gene="rplM"
                     /locus_tag="ACIYSB_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGC5427689.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(90088..91215)
                     /gene="zapE"
                     /locus_tag="ACIYSB_000573"
     CDS             complement(90088..91215)
                     /gene="zapE"
                     /locus_tag="ACIYSB_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGC5427690.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            91409..91807
                     /gene="zapG"
                     /locus_tag="ACIYSB_000574"
     CDS             91409..91807
                     /gene="zapG"
                     /locus_tag="ACIYSB_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGC5427691.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            91961..93328
                     /gene="degQ"
                     /locus_tag="ACIYSB_000575"
     CDS             91961..93328
                     /gene="degQ"
                     /locus_tag="ACIYSB_000575"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGC5427692.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            93418..94485
                     /gene="degS"
                     /locus_tag="ACIYSB_000576"
     CDS             93418..94485
                     /gene="degS"
                     /locus_tag="ACIYSB_000576"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGC5427693.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(94548..95486)
                     /gene="mdh"
                     /locus_tag="ACIYSB_000577"
     CDS             complement(94548..95486)
                     /gene="mdh"
                     /locus_tag="ACIYSB_000577"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGC5427694.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            95921..96391
                     /gene="argR"
                     /locus_tag="ACIYSB_000578"
     CDS             95921..96391
                     /gene="argR"
                     /locus_tag="ACIYSB_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGC5427695.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        24134 a        25634 c        23737 g        23016 t
ORIGIN      
        1 ctataatgcc gaacagtttc gtaatattgg cgagcgtagc ccaaaaattc cattcactat
       61 tcctttagtt aactgcccaa tgactgggtg ggagcacgat aacggcaacg ttgaagcgtc
      121 tttccgtcta tggctggaaa cgcgagataa tggcaccgtc cctaacttcc ctaatctggc
      181 aaaagtaggc tcttttgctg gcatagcggc aacaggtgtg ggtatccgta ttgacgatgc
      241 ggaaagcgga aacattatgc cactgaatgc tatgggcaat gataacacgg tttatcagat
      301 cccggcggaa tccaatggta ttgtcaatgt tgacctcatc gcttactacg tatcaaccgt
      361 agttccatca gaaatcaccc caggggaagc agacgctatc gttaacgtaa cgctggatta
      421 ccgttaatac gttagggcgt tatctgacct gtcagataac gcccttttcc ttcctctttc
      481 tcgttgtatc aggttgaaaa ataactatgt caaaacgaac attcgcggtg atattaacct
      541 tgttgtgtag cttctgtatt ggccaggcgc ttgcaggagg aatcgtttta cagcgaacgc
      601 gagtgatcta tgatgccagc cgcaaagagg ctgcgttacc tgtcgcaaac aaaggcgcag
      661 aaacgcctta tttactgcaa tcatgggtag ataatataga tggtaaaagc cgtgccccat
      721 ttattataac cccaccgcta tttcgtcttg aggctggcga tgactcatca ctgcgaatta
      781 ttaaaacagc tgataacctg cctgaaaata aagagtcgct gttctacatt aatgttcgtg
      841 ccattccagc aaagaaaaaa tcagatgatg ttaatgctaa cgagttgacg ctggtattta
      901 aaacacggat caaaatgttt tatcgccccg cacacctgaa gggacgggta aacgatgcgt
      961 ggaaatcact ggaatttaaa cgtagtgacc attcactcaa tatatataac ccaactgaat
     1021 attacgtcgt atttgccgga ctggcagtcg ataaaaccga tctcacaagc aaaattgaat
     1081 atatcgcgcc cggagaacat aaacagttac cacttcctgc atctggcgga aagaacgtga
     1141 aatgggctgc gatcaatgat tatggcggca gttccgggac agaaactcgt ccactgcgat
     1201 aaaaaatata aaaacacagg tcatcaggga atgccacaac gacaccacca gggacataaa
     1261 cgcacaccga aacagttggc gctcatcatc aaacgttgtt tgccgatggt gctcactggc
     1321 agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc cattatgctg
     1381 gaaaccacaa aaagtggtat gcaaacaacc gatctgtcgc gtttttcaaa aaaatatgca
     1441 caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt ttcacagaaa
     1501 aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac ggtagaacaa
     1561 ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa agatgacgat
     1621 agcgtgatca actcgcttga acaaatcatt cccggtacag ctgctgaatt tgatttcaat
     1681 catcagcaac ttaatttgag cattccccaa attgcactgt accgtgatgc aagaggttac
     1741 gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta ctcgtttaca
     1801 ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct gaatatgcaa
     1861 aatggtgcta attttggccc ctggcgatta cgcaactatt ccacatggac acgcaacgat
     1921 cagacatcaa gctggaatac catcagtagt tatttacaac gtgatatcaa ggcgttgaag
     1981 tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag ctacaccttt
     2041 actggcgtgc aactcgcttc cgacgataat atgttgccaa acagccagcg cggatttgcc
     2101 ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca aaatggttat
     2161 gtgatctatc aaagcaacgt gtcagcgggt gcctttgaaa ttaacgatct ctacccctct
     2221 tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacaca acgtcgcttt
     2281 atccagcctt attcttcatt acccatgatg cagcgacctg ggcatctaaa gtatagcgcg
     2341 accgctggac gctatcgcgc tgatgcaaac agtgatagca aggaacccga atttgctgaa
     2401 gccacggcaa tatatggttt gaataatact tttacgctgt atggcggcct gctcggttct
     2461 gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact gggcgcgttg
     2521 tcgatggata tcaacagagc tgacacccaa ttcgataacc agcactcttt tcatggctat
     2581 caatggcgta cgcagtacat caaagatatc ccggaaacca acaccaatat cgctgtcagc
     2641 tactatcgct ataccaacga tggctatttt agttttaatg aagccaatac ccgcaattgg
     2701 gactataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac aatatttgat
     2761 ggggtaagtc tgtatgcctc cggttcgcag caagactatt ggggcaataa cgataaaaac
     2821 aggaatatct ctgttggggt ttccggccag caatggggag ttggttacag cctgaattat
     2881 caatacagcc gctacactga tcaaaataat gaccgcgcac tctctttgaa tctcagtatt
     2941 ccgttagaac gctggttacc gcgtagccgg gtttcctatc agatgaccag ccagaaagat
     3001 cgcccaaccc aacatgaaat gcgtcttgat ggctcactgc tggatgatgg tcgcctgagc
     3061 tatagcctgg aacaaagtct ggatgacgat aacaaccata acagtagcct gaacgccagt
     3121 taccgttcac cttatggcac cttcagtgcc ggatacagct acggtaatga cagcagccaa
     3181 tacaattacg gcgttaccgg cggcgtggtt atccatcctc atggcgtgac gctctcgcaa
     3241 tatctgggca acgcttttgc gcttatcgat gctaacggag catctggcgt gaggatacaa
     3301 aactatccgg ggattgctac cgatcctttt ggctatgcag tggttcctta tctcacaact
     3361 tatcaggaaa accgtctctc ggtagatact acgcagctgc ccgataacgt cgatcttgaa
     3421 caaacaacac agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc
     3481 aatatcggtt atcgcgtact tgttacagtc agcgatcgca acggtaaacc gttgcccttt
     3541 ggcgctcttg ccagcaacga tgatacgggg caacaaagta tcgtcgatga gggcggcata
     3601 ctatatctct ctgggatatc gagtaaatca caaagctgga ctgtacgctg gggaaatcag
     3661 gcagatcaac aatgtcagtt tgctttcagt acaccggatt cagaacctac aacctctgta
     3721 ttacaaggca cagcgcagtg ccattaagga taaaaaaatg aaaagagcgc ctcttataac
     3781 aggtcttttg ttgatatcca catcctgcgc ttatgcctcc tcaggagggt gtggagccga
     3841 cagcactagc ggtgcgacaa attacagcag tgtggttgat gatgttacgg tgaaccagac
     3901 agataacgtg acaggacggg agtttacctc tgcaacgcta agtagcacta actggcaata
     3961 cgcctgttcc tgctctgcgg gtaaggcagt taaacttgtc tatatggtca gccccgtact
     4021 taccaccact ggacatcaga caggatatta caaactcaat gacagcctgg atattaaaac
     4081 cacattacag gcaaacgaca ttccaggact cacaaccgac caggttgtct ctgttaacac
     4141 ccgattcaca cagataaaaa acaacacggt atattctgct gcaacccaaa cgggtgtttg
     4201 ccagggtgat acgtctcgtt atggacccgt taatattggt gcgaacacca cctttaccct
     4261 gtatgtcacc aagccatttc tcggctcgat gaccattccg aaaacggata ttgccgtcat
     4321 taaaggcgcg tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc atgatttagt
     4381 caagttatcg attcagggaa atctcaccgc cccacagtcg tgcaaaatta atcagggcga
     4441 tgttattaag gttaattttg gattcatcaa tggtcagaag tttaccaccc gcaatgccat
     4501 gccagacggt tttactccag tagactttga tatcacttat gactgtggtg atacttcaaa
     4561 gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag accagtacaa
     4621 cctggtcgcc aggcgaagaa gttcagacaa tgtgcccgat gtcggtattc gtattgaaaa
     4681 tctcggcggc ggagttgcaa atattccttt tcagaacggt atccttcccg ttgatccttc
     4741 cgggcatggc accgtcaata tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga
     4801 aacaggaaaa tttcagggca cagccaccat taccgtcatc gtgcggtaaa agaagacgtt
     4861 gcgttttgat taacgcaacg cttcggcact gttacccctg ctgctccagc gcatacttat
     4921 acagcgcatt tttcttcacg ccgtgaattt ctgcggccag cgccgccgct tttttcagcg
     4981 gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct
     5041 gtgctttatg accttcgaca atcagcacca tttcgccttt gcgacggttt tcatcttcct
     5101 ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag gttttggtca
     5161 gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata tcttccaggc
     5221 tatctaacag acggtgggta gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg
     5281 cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttcg taacagaaac
     5341 ggtcagaggg taaacccgct gcgcttaacg cagtgatagc agcacacggc ccgggtagcg
     5401 gcaccacgcg gatccccgct tcacggcagg tacgcaccag atggtagcca ggatcgttaa
     5461 ttagcggcgt tccggcatcg gaaaccagcg caatgttttg cccctcttgc agcttcgcca
     5521 gcagcgtttc agctttttgt tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa
     5581 tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca atcagatcaa
     5641 cggcctgtaa tacctctaac gcacgctggg tgatatccgc cagattgccg attggcgtcg
     5701 gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc attgtgtcgt
     5761 ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa tatcactgga tacattatgg
     5821 taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc
     5881 tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat atgcagggca
     5941 cggcgcaggc tgattctgcc ttttatcttc agcagatgca gcaaagctct gatgatacca
     6001 ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa accgggcagg
     6061 cggttgagtt gtttaaccaa ctaccgcaag aactgaacga tgctcagcgt cgcgagaaaa
     6121 cactgctggc ggtagagatt aaactggcgc agaaagattt tgctggcgcg caaaacttgc
     6181 tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcgcgttac tggcaggcaa
     6241 aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta attgctcagg
     6301 aaccgctgct tggcgcgaaa gaaaaacagc agaatattga tgccacctgg caggcgctct
     6361 cctccatgac tcaggaacag gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc
     6421 aaggctggct ggatctgcag cgcgtctggt ttgataaccg taacgatccc gacatgatga
     6481 aagccgggat cgccgactgg cagaaacgtt atccgaacaa tccgggcgcg aaaatgctgc
     6541 caacgcagtt ggttaacgta aaagcgttta aaccagcctc gaccaacaaa atcgccctgc
     6601 tgttgccact gaatggccag gcagcggtat ttggtcgcac tattcagcaa ggctttgaag
     6661 cggcgaaaaa tatcggcact cagccagtgg cagctcaggt agctgccgca cctgccgcag
     6721 acgtagctga acaacctcag ccgcaaaccg tggatggcgt tgccagcccg gcacaagcct
     6781 cggttagcga tctgaccggt gaacagcctg cagcccagcc ggtgcctgta agcgccccgg
     6841 cgacaagcac cgcagcggta agcgcacccg caaatccatc cgcagagctg aaaatctacg
     6901 atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat ggcgcgagta
     6961 ttgtggtcgg tccgttgctg aaaaataacg ttgaagagtt gctgaagagc aacactccgc
     7021 tgaacgtact ggcactgaac cagccggaga atatcgaaaa tcgcgtcaat atttgttact
     7081 tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta
     7141 aacaagcgcc gctggtgctg atcccacgca gttcattggg cgatcgcgta gccaatgcgt
     7201 ttgcgcaaga gtggcagaaa ctgggcggcg gcaccgttct gcaacaaaaa tttggttcca
     7261 ccagcgaatt acgcgcgggt gttaacggcg gttctggtat tgctttaacg ggtagcccga
     7321 ttactctcag agcgacaacc gactccggca tgacgaccaa caatccaacg ctgcaaacca
     7381 cgccaaccga tgaccagttc accaataatg gcggtcgtgt cgatgcggtg tacattgtgg
     7441 caacgccggg tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac ggtagccaga
     7501 gcggtgcaac gctgtacgcc agctcccgca gtgcgcaagg gaccgctggc ccggatttcc
     7561 gactggagat ggaaggcttg cagtacagcg aaatcccgat gctggcaggc ggtaatctac
     7621 cgttaatgca gcaggcactc agcgcggtga ataacgatta ttcactggct cgcatgtatg
     7681 cgatgggcgt cgatgcctgg tcgctggcaa atcatttctc acaaatgcgc caggttcagg
     7741 gttttgaaat caacggtaat accggaagcc tgacggctaa cccggattgc gtgattaaca
     7801 ggaacttatc atggctacag taccaacaag gtcaggtagt ccccgtcagt taaccaccaa
     7861 acagaccggc gatgcgtggg aagcacaagc gcgtcgctgg ctggaaggca aaggactgcg
     7921 gtttatcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg
     7981 ccggaccacc atttttgtcg aggtacgcta tcgccgctct gcgctttatg gcggcgcggc
     8041 agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct tgtggctcgc
     8101 gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag ccttcaccgg
     8161 gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa ggtttaagga
     8221 ttagcgtgca agaaagaatt aaagcttgct tcactgaaag cattcaaact caaattgcgg
     8281 cggcagaggc gcttccggat gccatctccc gtgcagccat gacgctggtt cagtctctgc
     8341 tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat gcacagcatt
     8401 ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct gccattgcac
     8461 taaatactga taatgttgtc ttaacggcga ttgccaacga tcgcttacat gatgaagtgt
     8521 atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc atttccaccc
     8581 gtggcaacag ccgcgatatt gttaaagcag ttgaagccgc cgttacgcgt gatatgacca
     8641 ttgtggcatt gaccggctat gacggcggcg aacttgcagg tttgttaggg ccacaggatg
     8701 tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat atgctgacgg
     8761 taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat gattaaggag
     8821 aatacatgaa ggcattatcg ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt
     8881 gtgttgccgc tgccgtagtg ggtaccgctg ctgtgggtac caaagccgca actgacccac
     8941 gcagtgtcgg cacccaggtg gacgatggta ccctggaagt gcgcgtgaac agcgcattgt
     9001 cgaaagacga acagattaag aaagaagcgc gcattaatgt aacggcctat cagggcaaag
     9061 tgctgctggt tgggcagtca ccaaatgctg aactttcggc tcgcgccaaa cagattgcta
     9121 tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag ccgattggtc
     9181 tgggcgaagc atctaacgat acgtggatca ccaccaaagt gcgttcgcag ctcttaacca
     9241 gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga
     9301 tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc cgggtgagcg
     9361 gcgtgaagcg ggtaactacg gcgtttacgt ttattaaata gcagcccctt gtaatgcctg
     9421 atgcgacgct tgccgcgtct tatcaggcct acaacgatac aaaccgtagg tcagataagg
     9481 cgtttacgcc gcatccgaca tgttttccct caaatcagaa caacagcgcc agcccgccga
     9541 caatcacacc agacactgcc gccatcgccc ctgtcagcca taaggctttc gccgggaacg
     9601 ctttgcgcag cataatcagt gacggcaaac tcactgccgg gagcgtcatc aacaatgcca
     9661 gcgccggagc ggttcccata cctgccagca tcatcgtttg tacaatcgga atttctgctg
     9721 ccgtgggaat gacaaacaag catcctgcta ccgccatcgc caccacccac atcaggctgt
     9781 tatcgacagc accatcggca tgggggaata accagacgcg agcggcaccc aacaccagta
     9841 ctgcaaggat gtaaaccggg atcgtactcc agaaaagcgt ccatagcgcc ctgccccagc
     9901 ggctaaaaaa cccgccctgt gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg
     9961 gtgtttcacg cacccatttt tgcaccagcg tcgcaatcag caacaccatc accagcccgg
    10021 ccaccagacg aatcgccgca aaaccccagc cgaggacaaa gcccataaac accagcgtcg
    10081 ccgggtttaa caccggattg cccatccaga atgccagcgc accgcccatc gacacctgtt
    10141 ggcgacgcat tcccgccgcg accggagccg cacagcaggt acacatcatg cccggcaacg
    10201 aaaacagcgt tcccagcagc gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc
    10261 agtcacgcgg gatcagcacc tgaatcaacg aaccgaggat cacccccagc accgccgctt
    10321 tccataccgc gaggaaatag atcatcgcgt aatccaacgc cgcctgccat gggttagcat
    10381 ccgcctgcgc aaggatagat ttaccgatac tgtgggtttc ggcagcagta aacgctttgc
    10441 cgtagtaagg ttcccatttc acataccaga gaccggcaat gacaacgaga aagaaaagcg
    10501 cgggtttcca ccactgaatg ggcgttgccg cctgagatga agactgacca gtcatagcat
    10561 tccccaggaa taagttatga taattgagcg cgtgaatatt acgctcacta tcaattcttg
    10621 ggaataatta ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat
    10681 gctcaggccg catcgactct gccagcatga cccgcgccac gtctctcgca tcaatggatt
    10741 tccagttacc tggtaacaaa cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt
    10801 tgctacgatc gcccagtaac atcgacgggc gagcaatggt caatttcggc cagttctggg
    10861 cgattaatgc ttcttccatc tcccctttga cgcggttata gaaaaacggc gagtgggcat
    10921 tggcacccat cgcactgact accaacatat gctgcgcgcc cagtcgccgc ccggttaatg
    10981 cggtatccac taccagcgtg taatcggcat gaataaacgc ctctttgctc cccgcttctc
    11041 gccgcgtggt gccgagacaa caaaacacaa tgtcgatggg atcggtgacc tgcgccaacg
    11101 cgtcactcag ttgcggatca tggggattaa acaccccggg catatcgccc aacggacgtc
    11161 gcgtcggcgc agcaatggcg ttaactttcg gttcgttaat caacatccgc agcaggtgac
    11221 cgcccaccag ccccgttgcg cctgtaatca gtacctgact catcttcgct cctttacaga
    11281 attgtctgcc ttgcgctcca cggctcatgc accaggctta atagaccatg aggtaattat
    11341 ccccgattgt gggaaattcg cctcatccaa tgcaacaacg cggaggaagc atgagtaaga
    11401 aaattgccgt tttaatcact gatgaatttg aggattcaga atttacttca cccgcagacg
    11461 agttccgtaa agccggacac gaagtgatta ccattgaaaa acaagcgggt aaaacggtga
    11521 aaggcaaaaa aggagaagcc agcgtgacca tcgataaatc catcgatgaa gtgacgcctg
    11581 cggagtttga tgccctgctg ctaccgggcg gccattcacc ggattatctg cgtggggaca
    11641 accgttttgt cacctttacc cgtgattttg tgaatagtgg caaaccggtg tttgccatct
    11701 gtcacggccc gcagttgctg atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag
    11761 ttaaaccgat cattattgat gttaaaaatg cgggcgcgga attttacgat caggaagtcg
    11821 tggttgataa agatcagctg gttaccagcc ggacaccgga cgatctgcca gcgtttaacc
    11881 gcgaggcgtt acgcctgctc ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag
    11941 cccagcgtgt tgtcggtgaa tttgatttca tcgagccgga tttcccacac cggggccgac
    12001 agcattctgg caaccggaaa gcgacgattg tacgccttgc gcgcgaggtc gctttcctca
    12061 ccttccagcc tgcggatctc acctttaaac tgcacaccgc gaattaacgc taccgttttc
    12121 ggctgaccgt ttaccgttcc ggcaaccgca gcctgcggcc cactcatctg cgcgtggcgc
    12181 gttttttctt ccgtcaaaat gtagaaggcg actttctgcg catcaaaaag ataaaaggca
    12241 ttagcgcacc agagttcccc ttcctgctgc acacaccaag tgacaacatg ttgttttgcc
    12301 agccaacggc tgatggcgat gagtgtttcc attgctgttc tcttttatac tgtgggcctg
    12361 accttaacat gcgacgcaga cgatgacacc ctggtttctt tacttgatcc gtaccgccga
    12421 caataagctt tataccggga tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag
    12481 cggcaaaggg gcgaaagcac tgcgcgggaa aggagaacta acgctggtgt tttccgcgcc
    12541 agtgggcgat cgttcgctgg cgttacgggc ggaatatcgc gttaagcaac tgaccaaacg
    12601 gcaaaaagag cgtctggtag cggaaggcgc agggtttgca gagctgttaa gcagtctgca
    12661 aaccccggag attaaaagcg attgaaatgc tcgtgatact caaccaggcc ggtaacgcca
    12721 ttcagcgcgt catccgctaa acgatgtacc tggaaggcgc tttcagtgcc cggccagcgg
    12781 caacgcagat cgtgatgcgc cgccagttca aagccgaaac gactgtacag cgccggatcg
    12841 cccagcgtca ccactgcggc atagccgaac tcattaagcg aatcgagtcc ttcatagacc
    12901 agttggcgtg ccagcccctg tccacggtat ttttcatcaa ccgccaacgg tgccatgccg
    12961 acccattgca ggtcttcgcc ctgcacatca accggactaa atgccacata gccaatgacc
    13021 tgaccttcgt catctgtcgc caccagcccc agcgtcagaa agccatcttc acgcagatcg
    13081 tgaaccagct tcgcttccgc atcactttcg aatgagcgac gcagcagggc atcaataccc
    13141 ggcgcatcaa tgggaatttc tactcgaatt agcatggttc acctaccgat gtctgtttgg
    13201 tttcaggcgc ggttttcatt cccgcctcaa caaaatccgc cagttgcagc agcatcatgc
    13261 gtaaggcttt cggcatttgc tccagttcaa tggcgtccat caggtttttc acatacagcc
    13321 ccagctccgt atcgccttca atcaccagcc gacgctggaa gaagagcgta tccggatctt
    13381 gtttacgcgc cgcaatcatc agcagatcgc tggcgtcggc actaaaactc acatcagctt
    13441 gcgcgttctg gctaacgacc agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa
    13501 tatcacgcac atgaatactt aaccagcggc cttcaagaaa ctccagctcg ccatcatcca
    13561 gcgcctggcg gaattgccag cttaagacct gctcaagaac ctggcgtttt agcgcaaatg
    13621 gcgtcagttt taccggtaca ctcaacagag atggccccaa atgcacaata cgggaacgca
    13681 gtttatccaa cacgagtttt actccctgtt tcaacaatca tcctattttg ccatatcaga
    13741 aaaataacat agcggtataa atcaacaatt ccatatgaaa ttgctgctac caccaataca
    13801 actttaactg ccttaaatca aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt
    13861 aacatttttg cgttttgata gcgcaacctt caggaaaaat tatggagctg ctctgccctg
    13921 ccggaaatct cccggcgctt aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg
    13981 ggctaaaaga tgataccaat gcccgtcact tcgccggcct taactttacc gagaaaaaat
    14041 tgcaggaagc ggtgagtttt gtccatcaac atcgccgcaa acttcacatc gcgattaaca
    14101 cttttgcgca tccggacggt tacgcccgtt ggcagcgcgc cgtggatatg gcggcgcagc
    14161 tgggtgccga cgcgctgatc ctcgccgacc tcgccatgct ggagtacgcc gccgagcgtt
    14221 atccgcatat tgagcgccac gtatcggtgc aggcttcggc gaccaatgaa gaggcgatta
    14281 acttttatca tcgccatttt gacgttgctc gcgtggtgct gccgcgcgtg ttgtcgattc
    14341 atcaggtgaa acaactggca cgggtcacac ctgtaccact ggaagtcttt gctttcggca
    14401 gcctgtgcat tatgtcggaa ggtcgttgct atctgtcgtc gtatctgacg ggtgagtcgc
    14461 ccaacaccat aggcgcgtgt tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg
    14521 ggctggaatc ccgcctgaac gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag
    14581 gttatccgac gctatgtaaa gggcgttatc tggtggacgg cgagcgctat cacgcgctgg
    14641 aagaaccaac cagtctcaat accctggaac tgctgccgga gttaatggcg gcgaatattg
    14701 cttcggtgaa aattgaaggc cgccagcgta gcccggcgta tgtcagccag gtggcgaaag
    14761 tctggcgtca ggctatcgac cgttgtaagg ccgatccgca aaactttgta ccgcaaagcg
    14821 cgtggatgga gacgctcggg tcgatgtccg aaggcacgca aaccactctt ggcgcatatc
    14881 accgtaaatg gcagtgagaa aagcaatgaa atattcctta gggccagtgc tgtggtactg
    14941 gccaaaagag acgctggaag aattttatca gcaggccgcc accagcagcg ccgacgtgat
    15001 ttatcttggt gaagcggtat gcagcaagcg tcgggcaacc aaagttggcg actggctgga
    15061 gatggcaaaa tcgctcgccg ggagtggtaa gcagattgtg ctctccacgc tggcgctggt
    15121 gcaggcatca tctgaactgg gcgaactgaa acgctatgtt gagaacggtg agtttctgat
    15181 tgaagccagc gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc
    15241 cgggcacgcg ctgaactgct acaacgcggt gacactgaaa atattgctca aacagggcat
    15301 gatgcgctgg tgtatgccag tggagctttc ccgcgactgg ctggtgaatc tgcttaatca
    15361 gtgcgatgag ctgggcattc gtaaccagtt tgaagtggaa gtcctgagct acggtcatct
    15421 gccgctggcc tactccgccc gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga
    15481 gtgtgaaacc tgctgcatta agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca
    15541 acaagtgttt gtactcaatg gcattcagac catgagcggc tacgtttaca acctcggtaa
    15601 cgagctggca tccatgcagg gcctggttga tgtggtccgc ctgtcaccgc agggtactga
    15661 cactttcgcg atgctcgacg ccttccgcgc taatgaaaat ggcgctgcac cactgccgtt
    15721 gacggcgaac agcgactgta acggctactg gcggcggctg gcaggactgg agctgcaagc
    15781 ctaagtaaat agctcacttt gttaacaact ttaactactc tttaatgcag tattaaagat
    15841 taatcggtaa caaagtgagc tgttatgact gataaaacca ttgcgttttc gctactcgat
    15901 ctggccccca ttcccgaagg ttcttcagcg cgagaagcat tctcccactc tctcgatctc
    15961 gcccgtctgg ctgaaaagcg cggctatcat cgctactggc tggcagaaca ccacaatatg
    16021 actggcattg ccagtgctgc cacgtcggta ttgatcggct atctggcggc gaataccacc
    16081 acgctgcatc tggggtctgg cggcgtgatg ttgcctaacc actcaccgtt ggtcattgca
    16141 gaacagttcg gcacgcttaa tacactctat ccggggcgaa tcgatttggg gctgggtcgt
    16201 gctccgggta gtgaccaacg gacaatgatg gcgctacgtc gtcatatgag cggcgatatt
    16261 gataatttcc cccgcgatgt ggcggagctg gtggactggt ttgacgcccg cgatcccaat
    16321 ccgcatgtgc gcccggtacc aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc
    16381 agcctttaca gcgcgcaact ggcggcgcag cttggtctgc cgtttgcgtt tgcctcacac
    16441 ttcgcgccgg atatgctgtt ccaggcgctg catctttatc gcagcaactt caaaccgtca
    16501 gcacggctgg aaaaaccata cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac
    16561 cgcgacgctg aatttctgtt tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc
    16621 gaaaccgggc aactgccgcc gccgattcaa aatatggatc agttctggtc accgtctgag
    16681 cagtatggcg tgcagcaggc gctgagtatg tcgttggtag gtgataaagc gaaagtgcgt
    16741 catggcttgc agtcgatcct gcgcgaaacc gacgccgatg agattatggt caacgggcag
    16801 attttcgacc accaggcgcg gctgcattcg tttgagctgg cgatggatgt taaggaagag
    16861 ttgttgggat agtgtgtctt aacgcgggaa gccttatccg agctggcaac gctgtcctac
    16921 atagacctga taagcgaagc gcatcaggca ttgtgtaggc agcagaaatg tcggataagg
    16981 caccgctgat tactgataca ccggcagtaa attaaagctc gataaaatat gcaccagtgc
    17041 gttgccgacg ccaaacacca gaatcagcgc aatcatcggc ttgccacccc agacgcggaa
    17101 tttcgggctg ccaaagcgtt tacgcgatgc acgggctaac agcgccggaa caattgccgc
    17161 ccagatggta gccgctaaac cagcataacc aatggcgtac aggaatccgt tcgggaacaa
    17221 cagccccccc acaactggcg gggcaaaggt cagcaatgcc gttttcaagc ggcccacagc
    17281 cgagtcgtcg aaaccaaaca gatctgccag atagtcaaac aaacccagcg ttacgccgag
    17341 gaacgaactc gctaccgcaa agtttgagaa cacgaccagc agcagatcca gactacggct
    17401 gttcagtacg ccgcttaacg cctgtaccag cacatcaata ttaccgccct tctctgcaat
    17461 accgataaac tccggacgcg ggatgttacc catcgtcgcc agcaaccaga tggtatacag
    17521 cgccagcgcc atcagcgtac cgtacaccag acatttcacg atggttttcg gatctttgcc
    17581 gtaatacttc atcaggcttg gcacgttacc gtgataacca aacgatgcca gacagaacgg
    17641 cagggtcatc aacagatacg gtgcataaga cgcattgctt tcggcgacgt tgaacaatgt
    17701 cgcaggctgc acatgcccca gcaggctacc aaaggtgagg aagaaggtaa tgactttcgc
    17761 ccccagcaca atcgctgtca tgcgactgac ggctttagtg ctcaaccaca ccacaaacgc
    17821 taccagcaat gcaaaaccaa aacccgccgc ccgtgccggg acgtttagtg acatctctgc
    17881 gaaggtgtga tgcagaatcg aaccactggc agaaatatag gcataggtca ggatatagag
    17941 cacaaaggca atggaaatgc cgttgaccac gttccagcct ttgcccagca aatctttggt
    18001 gatggtgtca aaactcgaac cgattctgta attcaggtta gcttccagaa tcatcaagcc
    18061 ggaatgcagc atacagaacc aggtaaagat cagcgccgcc attgaccaga aaaaccacgc
    18121 cccggacatg accactggca gagaaaacat ccctgcgcca ataatggtgc cgccgataat
    18181 caccacgccg ccaagcagcg acggtgacgt ttgggtggtg gttagtgttg ccatgagggc
    18241 ttctctccag tgaaaaatag tgcgactgcg ttgttatgca ttgcactgta ccagtacacg
    18301 agtacaaaag acagaaaaaa agccccgatg gtaaaaatcg gggctgtata tattatttta
    18361 cagattgtgt tcgctgttca gcgatgatta cgcatcacca ccgaaacgac gacgaccggt
    18421 agaatcatca cgacgcggag cgcggccttc acgacgttcg ccgctaaaac gacgaccatc
    18481 accacggcca ccttcacggc gttcaccgct gaagttacga ccgccttcac gacgttcgcc
    18541 accgaaacca cgaccaccgc cacgacgctc accgccagta tgcggctgtg catcgcccag
    18601 taactgcatg ttcatcggct tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc
    18661 acccggcata cctttcggca gttcgatggt ggagtgagaa gcaaacagct tgatgttacc
    18721 aatgtaacgg ctgctgatgt cgccttcgtt agcaatcgca ccaacgatat gacgaacttc
    18781 aacaccatca tcgcggccca cttcaatgcg gtacagctgc atatcgccaa catcacgacg
    18841 ttcacgacgc ggacgatctt cacggtcacc acgcgggcca cggtcgttac gatcgcacgg
    18901 accacggtca tcacggtcac ggaattcacg tttcggacgc atcggcgcat ctggcggtac
    18961 gatcagagta cgttcaccct gtgccatttt cagcagtgcc gcagccagag tttcgagatc
    19021 cagctcttca ccttcagcag tcggctgaat tttgctcagc agtgcgcggt attgatccag
    19081 atcgctgctt tccagctgct gctgtacttt agcggcgaat ttttccagac ggcgtttgcc
    19141 tagcagttct gcgttcggca gttctacttc cggaatagtc agcttcatag tacgttcaat
    19201 gttgcgcagc agacgacgct cgcggttctc aacgaacagc agcgcgcggc cagcacgacc
    19261 cgcacgaccg gtacgaccga tacggtgaac gtaagactca gaatccatcg ggatatcgta
    19321 gttaactacc aggctgatac gctcaacgtc caggccacgg gctgcaacgt cggtcgcaat
    19381 caggatgtcc agacgaccat ctttcaggcg ttccagtgtc tgttcacgca gcgcctggtt
    19441 catgtcaccg ttcagcgcgg cgctgttgta gccgttacgc tcaagagctt cagccacttc
    19501 cagagtcgcg tttttggtac gaacgaagat aatcgccgca tcaaaatctt ccgcttccag
    19561 gaaacgtacc agtgcttcgt ttttgcgcat accccagaca gtccagtagc tctggctgat
    19621 gtcaggacgg gtagtcacgc tggactgaat gcgcacttcc tgcggctctt tcataaagcg
    19681 gcgggtaatg cgacgaatcg cttccggcat ggttgcagag aacagagcgg tctgatgacc
    19741 ttccgggatc tgcgccataa tggtttcaac gtcttcgatg aagcccatgc gcagcatttc
    19801 gtcagcttca tccagaacca gaccgctcag tttagagagg tccagagtgc cacgtttcag
    19861 gtggtccagc agacggcccg gagtaccgac aacgatctgc ggcccctgac gcagggcgcg
    19921 taattgcacg tcataacgct ggccgccgta cagagcaacc acatttacgc cgcgcatgtg
    19981 tttagagaaa tccgtcattg cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag
    20041 caccagaatc tgtggtgctt tcagctcagg atcaagattc tgcaacagag gtaaagagaa
    20101 tgctgcagtt tttccgctcc ccgtctgggc catacccaga acgtcgcggc cattcagcag
    20161 atgtggaata cactctgcct gaattggaga tggtttttcg taacccagat cgttaagggc
    20221 ttcaaggata ggagccttca ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta
    20281 gtacgtgtgc ctcaaaatta atggcggcca gtctacataa ctcatcatga aattgatcag
    20341 caattttcat tgaaaagtgt gaaccggctc aaagtaggtg tattaacgaa caacaacgcc
    20401 ctcacccgtt aaggtgatgg caatcaaaaa agattacggg ctgatgtgta cgtcagctat
    20461 tgctggtccg attctgccag gtcatcttgg tcctggccca ggagcgataa ttccaacaat
    20521 gcgtatcggt gctcaacaaa gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc
    20581 gtggcgctgt ccaaatcccc cagacttagg tagtacttac ctaaatagaa gttggtttca
    20641 ctgagatgct cagcgagcga ggtgttatcc gttgcgtccg ccttgagcct ttccattaac
    20701 gtttgttcgc taatgttgcc caggtagaac tcgacaatgt tccatcccca ctgttcctta
    20761 tccgattttt cgaagtgctg tttcaacact tctttagcct gcttctcatc gagcttctgc
    20821 tcggcgagat aaagccacag actacggaaa ggatcattgg gatcgtcttg ataaaacgcc
    20881 agcagatcat cttgcgctaa cttgtcacga ccgccgtaat ataatgcgat cccgcgattc
    20941 aagtgcgcgt agttgtaagt tggatcaagc tcaagtacag aatcaaacgc ttcataggca
    21001 gcatcaaaat tgcctgcctg cgttaaatat atgcctaagt aattgaatac ttcaggcata
    21061 tccggtcgga ttgccagcgc ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga
    21121 ctatcataca acactccgcg ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc
    21181 cgactggcaa ggatttgttc catacgtgcc agaatcactt cctgctgtaa agtcggttgc
    21241 aatggtaccg cgaggacttc acttttacgc caggaagtat tactgcatcc tgcaagcgta
    21301 agtgctgtcg caacgaaaca ccagcgcaaa aaaggcttca tttcccactc ccgaagacca
    21361 cggttgaatg aacgtcctgt tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc
    21421 cccgccgcag cggagggcaa atggcaacct tactcgccct gttcagcagc cggagcttcc
    21481 ggtgctgcag caggttgaga ctgctcagtc gcttctttaa tgctcagacg gatacggccc
    21541 tggcgatcaa cttccagaac tttcaccggt acttcctgac ccatctgcag gtaatcggtc
    21601 actttctcaa cgcgtttgtc agcgatttga gagatgtgga ccagaccttc tttaccgccg
    21661 ccgatggcaa caaatgcgcc aaagtcaacg atacgggtca ctttaccagt gtagacgcgg
    21721 cccacttcga tttctgcagt gatctcttcg atacgacgaa tagcatgttt cgctttctcg
    21781 ccgtcggtcg ctgcaatctt cacagtaccg tcatcttcga tttcgatggt ggtgccagtt
    21841 tcttcggtca gagcacggat tacagaaccg cctttaccga taacatcttt gatcttgtcc
    21901 gggttgatct tgatggtatg gatacgcggt gcgaactcag agatatcgcc acgcggcgcg
    21961 ttgatcgcct gttccattac gcccaggata tgcagacgcg cacctttagc ctggttcagc
    22021 gcaacctgca tgatctcttt ggtgatacct tcaattttga tatccatctg cagtgcagag
    22081 ataccgtcgc gggaacctgc aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc
    22141 aaaatgtcag acagtacaac gtagttgtcg ccttctttca ccagacccat tgcgataccc
    22201 gcaacggcag ctttgatcgg cacacctgcg tccatcagcg ccagagacgc gccgcacacg
    22261 gaagccatag aagaggaacc gttggattca gtgatttcag acacaacacg tacggtgtac
    22321 gggaatttgt ccatatccgg catgactgcc agcacgccgc gcttcgccag acgaccgtga
    22381 ccaatttcac gacgcttcgg agaaccgacc atgccggttt cgcctacgga gtacggaggg
    22441 aagttgtagt ggaacaggaa ggtatcggta cgttcgccca tcagttcatc aagaacctgc
    22501 gcgtcacgag cagtacccag cgttgcggta accagcgcct gcgtttcacc acgggtgaac
    22561 agcgcagaac cgtgagtacg cggcagcacg ccagtacgca catccagacc acggatcata
    22621 tctttttcac gaccgtcgat acgcggttcg cctgccagta cgcggctacg aacaacgttt
    22681 ttctcgatcg cgtgcagaat ttcacccagt tcgttttcgt ccagggtttc gtcttcagca
    22741 agcaacgtcg cgatggtttc agatttgatg acatcaacct gcgcataacg ctcttgtttg
    22801 tcggtgatgc ggtaagcatc gctcaggcga gcttcagcca gtgcagcaac gcgcgcgttt
    22861 agcgcttcgt ttaccggctc cggctgccag tcccaacgcg gtttaccggc ttctttcacc
    22921 agttcattga tgttctgaat aacaacctgc tgttgttcat gaccgaacac tactgcgccc
    22981 agcatctggt cttcgctcag cagttgagct tcagattcaa ccatcagtac agcggcttca
    23041 gtaccggcaa caaccagatc cagtttgctc tctttcagct cgtcctgagt cgggttcagt
    23101 acgtactggt cattgatgta acctacgcgg gcagcaccaa tcgggccatt gaacggaata
    23161 ccagacagag acagcgctgc ggaagcacca atcatcgcga cgatatccgg gttaacttgc
    23221 gggttaacag aaaccacggt ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac
    23281 agcgggcgaa tcgggcggtc aatcagacgc gcgatcaggg tttcgccttc gcttgggcgg
    23341 ccttcacgac ggaagaagct acccgggata cgaccagcag cgtaggtacg ctcctgatag
    23401 ttaacggtca gtgggaagaa gtcctgacct ggtttggctt ttttctggcc aacaacggta
    23461 acgaataccg cggtgtcatc catgctaacc ataacagcgg cagtagcctg acgagccatc
    23521 atgccggttt ccagagtcac ggtgtgttgg ccgtactgga atttacgaac gatcggatta
    23581 agcaatgtaa tatcctttct ctttcttaga cagtacctta cggcactggt gttaataccc
    23641 gatcttctgc gcatcctcgc gactaatgac aaccctaacc cagctctatg tgggtaaagc
    23701 ctctcattag ccgcgcgaac ctctgcaacg gaagatcatt catagcaaca atacattagt
    23761 ttccagtgaa ttgctgccgt cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa
    23821 actcgcaaga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
    23881 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
    23941 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
    24001 gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac
    24061 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
    24121 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
    24181 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacctgt tatcgcaaga
    24241 cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcaacgc ggccttcatc
    24301 gtcaatttcg cccataccga taaatttgcc gttctcacct tccgtgacgc gaaccagtcc
    24361 ttccagtggc gcaccagatg tacgaaccgg gttaccattt ttgaagtaaa cagaagacgt
    24421 taacggaaga ttcaccaccg gatagtccga agctggactg tccattggca tcagtaatgg
    24481 atcaagtaac tccgcggctg gaatatcctg ctgttcagct tgctcaacaa gttcacgcag
    24541 gtgctccagg gtcaccatcc gttcaaccgg atatttactt accgccagac ggcgcaggta
    24601 aataacatgc gcgccacagc cgagtttttc acccaggtca tcaatgatgg tgcggatata
    24661 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcatggc gaataaacag
    24721 caattcataa acggtaatcg gacgcgcttc acgcggaact tcaatgccct gacgcgcata
    24781 ttcgtacagt tttttgccct gatatttcag tgctgaatac atcgaaggga tctgttcgat
    24841 atcgccacgg aaagtatcca gtgccgctgc cagctgctct gcgctaaagg ttaccggacg
    24901 ttcttcaacg atctgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat
    24961 gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgag
    25021 gcaaatcggc aacatgccgg tcgccagcgg gtccagcgca ccggtatgcc cggcacggtt
    25081 ggcgttatat atacgtttca ctttttgcag cgcatcgttg ctggacatac cctgaggttt
    25141 atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    25201 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    25261 aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc
    25321 acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc
    25381 aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat
    25441 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    25501 aggcgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    25561 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    25621 gctgttagcc cctttttaaa attaatttca ggtggaaggg ctgttcacgt tgacctgata
    25681 agacgcgcca gcgtcacatc aggcaatcca tgccggatgc agcgtaaacg ccttatcccg
    25741 catggaaccc taaaaacctt aagcaatggt acgttggatc tcgatgattt cgaatacttc
    25801 gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc
    25861 gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta
    25921 gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt
    25981 aaccatacag cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc
    26041 cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac
    26101 ttcgtcaatc aggttataga tgacggagta gtaacgcaga tccaggcttt ccgcttcaat
    26161 cactttacgt gcagaggcat cagcacgtac gttaaagcca accaggatgg cgttggacgc
    26221 cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac
    26281 tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcga cagaaccctg
    26341 tacgtctgcc ttcaggacga tattcacttc gtgaacttcg ccttcggtca tgttggcgaa
    26401 catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg
    26461 acgatagagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc
    26521 cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag
    26581 cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag
    26641 aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg
    26701 acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt
    26761 cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga taccggtacc
    26821 cgctttcgca gatacgtgta cgaactggct ttcaccgccc cactcttccg gcaggatgcc
    26881 gtactgggag agttcgttct taacgcgatc cggatcagct tctggtttat cgatcttgtt
    26941 cactgcaacc accaccggta cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg
    27001 cggcatcaca ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc
    27061 acgagcacgc attgaagtaa acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc
    27121 gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga
    27181 ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc
    27241 catgatggtc acaaccggcg cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat
    27301 taccgcctct tccagctcgt tttcacgacg caggataact ttatggccca tctcttcagc
    27361 aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt
    27421 catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac
    27481 ggtgatagtt tcgccgatca caacgtcacg gttaacggcc tgagcaggct tctggaagcc
    27541 ttgctgcagc gaagaacctt tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc
    27601 ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt
    27661 cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg
    27721 gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt
    27781 atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc
    27841 ttcttcgagt ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg
    27901 ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac
    27961 tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc
    28021 tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg
    28081 acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc gctgcaaggc gttcagcctc
    28141 ttgcggatcg cgtttcacaa aggtgcgttt cttgcggact tcgatttgta ccgatttgct
    28201 ttttccaccg gtaccaggaa tgttaagggt gctgcgtgtt ttacgttgca gcgtcaattt
    28261 gtccgggcct gaatttttct gattcaggtg gtcaatcaaa gtctgtttct cttgtgcaga
    28321 cacagagtcg tcagcagact tccggatacc tgcatcagca aattgctgta ccaggcgttc
    28381 cacggaggtc tgtcgctctg cggccagcgt tttaatcgtt acatctgtca tgctgttcct
    28441 tcctgctaca gtttattacg cttcgtcacc gaaccagcaa atattacggg cagccataat
    28501 cagtgctccg gctttttcgt cggtcaaccc ttcgatatca gccagatcat caatgccctg
    28561 ttcggcgaga tcttccagcg tacaaacgcc acgggcggcc agtttgaatg ccaaatcacg
    28621 atctacccct tcaaggttca gcagatcgtc agccggtttg ttatcaccga ggctttcttc
    28681 ctgggcctgt gcaatggtgg ccagtgcatt tttagcacgc tcgcgcagtg cttcaacggt
    28741 cggctcatca aggccttcga tttccaacag ctctttcatc ggcacatagg ccagttcttc
    28801 cagcgtcgag aagccttctt ctaccagaac agtcgcgaag tcttcgtcga tgtcgagata
    28861 tttggtgaag gtgtcgatcg ctgcgtgcgc ttccgcctga tgcttagcct gcaggtcgtc
    28921 aacggtcatc acgttgagtt cccaaccgct cagctgcgaa gccagacgca cgttctgacc
    28981 gttacggcca atcgcctgcg ccaggttacc ggcttcaacg gcgatatcca tggtgtgttt
    29041 atcttcatcc accacgatag aagcaacgtc tgccggtgcc attgcgttaa tcacgaactg
    29101 cgccgggtta tcatcccaca ggacgatatc gatacgctcg ccacccagtt cagtagacac
    29161 cgcctgaaca cgcgcgccac gcatacctac gcaagcacct accggatcga tacgtttatc
    29221 gttggttttc accgcgattt tcgcacgaga acccggatcg cgagccgctg ctttaatttc
    29281 aatcacttct tcgccgattt ctggcacttc aatacggaac agttcgatca gcatttccgg
    29341 cttggaacga gtgacgaaca gttgcgcgcc acgcgcttcc gggcgaacgg aatagagcac
    29401 gccacgaacg cggtcgccag ggcggaagtt ttcacgcggc agcatatctt cgcgcaggat
    29461 cacggcttca gcgttgttgc ccagatccag agagatgttg tcgcggttta cttttttcac
    29521 cacgccggtg atgatttcac cttcgtgttc acggaactga tcaaccacca tcgcacgttc
    29581 ggcttcacgc actttctgca cgataacctg ttttgccgtc tgggtagtga tacggtcaaa
    29641 ggtaacagac tcaatctgat cttcaacgta atcgcccagg ttcaggcttt catcttcata
    29701 acgtgcggct tcaagggtga tttccttggt cggctgggtg acttcatcaa caactaacca
    29761 gcgacggaaa gtgtcaaaat caccgctttt gcgatcgatc tgtacgcgga cgtcgatctc
    29821 ttgttcatat tttttctttg ttgctgtcgc cagcgcgctt tccaatgctt cgaaaatctt
    29881 ctcgcgaggt agcgcctttt cattggatac ggcttcaact acagccaaaa tttctttgtt
    29941 catcgcgggc ttttcacctc atccagacta ttaaaagtgg ggaaccaggt tcgccttctg
    30001 gatattactc agcgcgaaca cttcatcttt accttcgacg gtaactgtga tcatttcacc
    30061 gtctaccgct ttgataacgc cctgccattt acgacggttt tgtaccgcca tacggagaac
    30121 cagagtcacc tcttctccga caaaacgggc gtagtgttca gccgtgaaca gtgggcgatc
    30181 gagacccggt gaggagactt ccaggttata agcaacggtg atgggatctt caacatccag
    30241 cacagcactt acctggtggc tcacatcagc acaatcatca acattgatgc catcttcact
    30301 atcaatatag atgcgcagtg tggatgtgcg accgcgaata aattcgatgc caaccagttc
    30361 aaaacccagg gcctcaactg gcgcagtaat catctctgtt aatttttgct ctaatgtgga
    30421 caagcccacc cccaagacat aaaaaaaggg cctaaagccc agttattctg tagtcagata
    30481 acaaaaaacc ccgataaatc ggggctttat ataactgaac cctataaccg caactgcggt
    30541 ctggagcact ttccagaagg attttttcaa atcccactac gaaggccgaa gtcttcacag
    30601 tatatttgaa aaaggactct aagggaaagt ggttgcgggg gccggatttg aaccgacgac
    30661 cttcgggtta tgagcccgac gagctaccag gctgctccac cccgcgcctg aaacgtggca
    30721 aattctactc gttttgggta aaaaatgcaa atactgctgg gatttggtgt accgagacgg
    30781 gacgtaaaat ctgcaggcat tatagtgatc cacgccacat tttgtcaacg tttattgcta
    30841 atcatgtgaa tgaatatcca gttcactttc atttgttgaa tacttttgcc ttctcctgct
    30901 ctcccttaag cgcattattt tacaaaaaac acactaaact cttcctgtct ccgataaaag
    30961 atgattaaat gaaaactcat ttattttgca taaaaattca gtgagagcgg aaatccaggc
    31021 tcatcatcag ttaattaagc agggtgttat tttatgacga cgattctcaa gcatctcccg
    31081 gtaggtcaac gtattggtat cgctttttct ggcggtctgg acaccagtgc cgcactgctg
    31141 tggatgcgac aaaagggagc ggttccttat gcatatactg caaacctggg ccagccagac
    31201 gaagaggatt atgatgcgat ccctcgtcgt gccatggaat acggcgcgga gaacgcacgt
    31261 ctgatcgact gccgcaaaca actggtggcc gaaggtattg ccgctattca gtgtggcgca
    31321 tttcataaca ccaccggcgg cctgacctat ttcaacacga cgccgctggg ccgcgccgtg
    31381 actggtacca tgctggttgc tgcgatgaaa gaagatggcg tgaatatctg gggtgacggt
    31441 agcacctaca aaggaaacga tatcgaacgt ttctatcgtt atggtctgct gaccaatgct
    31501 gaactgcaga tttacaaacc gtggcttgat actgacttta ttgatgaact gggcggccgt
    31561 catgagatgt ctgaatttat gattgcctgc ggtttcgact acaaaatgtc tgtcgaaaaa
    31621 gcctactcca cagactccaa catgcttggt gcaacgcatg aagcgaagga tctggaatac
    31681 ctcaactcca gcgtcaaaat cgtcaacccg attatgggcg tgaaattctg ggatgagagc
    31741 gtgaagatcc cggcagaaga agtcacagta cgctttgaac aaggtcatcc ggtggcgctg
    31801 aacggtaaaa cctttagcga cgacgtagaa atgatgctgg aagctaaccg catcggcggt
    31861 cgtcacggcc tgggcatgag cgaccagatt gaaaaccgta tcatcgaagc gaaaagccgt
    31921 ggtatttacg aagctccggg gatggcactg ctgcacattg cgtatgaacg cctgttgacc
    31981 ggtattcaca acgaagacac cattgagcag tatcacgcgc atggtcgtca gttgggccgt
    32041 ctgctgtacc aggggcgttg gtttgactcc caggcgctga tgctgcgtga ctctctgcaa
    32101 cgctgggttg ccagccagat cactggtgaa gttaccctgg agctgcgccg tgggaacgat
    32161 tattcaatcc tgaataccgt ctcagagaac ctgacctaca agccagagcg tctgacgatg
    32221 gaaaaaggcg actcggtgtt ctcgccagat gatcgtattg gtcaattgac catgcgtaac
    32281 ctggatatca ctgatacccg cgagaaactt ttcggttatg ccaaaactgg cctgctttcc
    32341 tcctctgccg cttcaggcgt gccgcaggtg gagaatctgg aaaacaaagg ccagtaattc
    32401 gacatcaacc ctgcccttca ggggtggggt taccttgctt atcctctgta aactcctttc
    32461 tgagcatcac ataatcgaaa acatcatggt ttgcatccac aacccgtgag tctcctttat
    32521 agtgtgcaat cacttcctca agcgtttgcg gctgttccgg ctttgttacc cccatccacg
    32581 cattaataag gtaattattg taagctgtcg aaaagatgtt gttttccgtt gtgcgatcca
    32641 cgccatcccc aagaacgggg ctataccaga taaacatcgg gacatgatat gcctgctggc
    32701 tagcctccct gcctccatga aaatagacgt tcttcttcgt agggtcacgt tccaggccgt
    32761 ggtcggcaaa atacatgacc gaggcgcggc gatcttttaa taattcaaaa acctgaccta
    32821 gcaaactatc tgtgtaatga atggagttgt catagcaggc atcctgatcg tcctgcggtt
    32881 gaaacacggc gctggattgc ggataggcgc tacaagccgg ttcatggctt ccatttaaat
    32941 gaagaacaat cagtttcttt tgctgcgtat tttgctgtaa tgcttgcgat aaatgcggca
    33001 acaacaattc atcaaatcct ctgacataaa ctgtttccat ggcgcgcatg gcgatactgg
    33061 taactgctgt accattctgc cgaaaagcgg attgcgagct tagccagaaa gtctgaaatc
    33121 ctgcttgatt agccatatta ataatgttgt cggggtaatt atgaatgtca tgactcaaaa
    33181 cagaatcagc agttaaagaa aggggaaccg acagcgcagt gtaaggtgcg ccgcttattg
    33241 cttgattaaa cagtttgatc tgttttcttt gtgcttcaac ttgcggtgtc gtagagcgtg
    33301 tatatccata caaagacata ttgtcgacac gtacagactc ccccacaatc aacacgtagg
    33361 tatcaatacc tgtatccctg actgataatt gaaaatacgg caccgtgttt gcaattgaga
    33421 gtaatctttg atgctctttc gctgctaaag caaaatagtt gagattgaaa aacggcgtat
    33481 aggtagcaaa tcgcgacgct agtatatatg gactgaacgc ttttttattt tttgcatctt
    33541 tataagcaaa ttgacaagcg gaaaataaac tgcccgaaat gacaatcagc aataatattc
    33601 ctgtcacttt ttttgtcggc aaggaaacat catattttat tattactgcc aaaaaaagaa
    33661 gggataaaaa agcaaaggca catagataag ggatatacat ccccagcatt ttgacaactt
    33721 catccggatc actctggagc acactaatcg caaatccatc attgaaagtt gtaccaaagg
    33781 tacaccatga atagaggcta atactcatgt ctgccgcagt cagtaacaca aatggtgcgg
    33841 caacaagcag gcgaagcgga agggatgata tacgttttac aaccaggatg gtcagaacga
    33901 agaacagcaa gcgctgaacc atatgcgctc cggaagaagc gactaagttc gtaataccaa
    33961 aaacaataac acaaagatac aacaaccaat gagatttaaa agttctagca aatttgttga
    34021 atactgtcat ccagaaatcc aagttcacaa ccaatataaa caattatcaa caatgtttaa
    34081 ttttatatca gacatgtttt tcaactctga tccatatcag aacgaaacac tgttagtttt
    34141 ttctttttaa atgatgcggc cattgcgttc tcagtaatta aataacatta tcaatgcgtc
    34201 tctaaacatt acgcagaacg tagccatcag tcgttatact gacaggagca ggatggttta
    34261 tcgagaagta aacatatccg tattcgtacc ggaactggag ttgacacaat aaagtgccaa
    34321 ttatgtcagt agaagggaaa aatttataac taaggcgtac cggcaccatc gtttcaaggt
    34381 accagctacg agtaaagcaa ctggacgaga tacagatacc tgacaaccat tcctcagacc
    34441 aggaccaaaa cgaaaaaaga cgcttttcag cgtctctttt ctggaatttg gtaccgagga
    34501 cgggacttga acccgtaagc cctattgggc actaccacct caaggtagcg tgtctaccaa
    34561 ttccaccacc tcggcacgga tactactttt tagttcggga tatcgctggt cggcttagcc
    34621 ggagcagctg gctgagtttg ttcggttttc gccggtgcac tcagattttc ccattcgcta
    34681 cctttattgg ttttgttgct attgatgtta cccagcacca gactgatgat gaagaataac
    34741 gttgccagca gcgccgtcat gcgggtcatg aagttaccag aaccacttga accaaacagc
    34801 gtagcggaag cgcctgctcc gaaggaggct cccatatcag cgcctttacc ttgctgcagc
    34861 atgatcagac caacaaggcc aattgccaca ataaggaaaa ctactaaaag agcttcatac
    34921 ataatcaacc tgttccttgc ggagttgccg cgtaccaatg cttcaaccaa taaagcggga
    34981 gtttttaatc tttcccactg aagcgggtgt gaatactaac caaagcgaat gaccttcgca
    35041 agggcaattt tatcgcattg tatcaactgc ggaaaaaaac agcaaaaccg atgtgttcgc
    35101 tgagaaaaaa ggcgacaatc gccgcctttt tagccagtta tctaacgctt taaacggctt
    35161 ttactgcatc ggcgatgcgg tgtgcaaatt cagtcacctg cgcttcgtct tcgccttcca
    35221 ccatcacgcg aattaacggt tcggtgccgg atttacgcag caacacgcgt ccacggttgc
    35281 ccagcgcagc ttcaacctct gcggtcacgg ctttaactga ctcatgctca agtggatcgc
    35341 cgctacctgc ggtgtaacgt acgttaacca gaatctgcgg gaacattttc atgccgctgc
    35401 aaaggtcgtg caggctcata tggttacgtg ccatcgccgc cagcacctgc aagccagcaa
    35461 cgatgccgtc accggtagta gttttatcca gcaggatcac atgaccggaa ttctctgcac
    35521 cgatacgcca gcctttctcc tgcatttttt ccagtacgta gcggtcaccc actttcgcgc
    35581 gcgcaaatgg aattcctaac tgtttcagcg ccagttcaag ccccatgttg ctcatcaatg
    35641 tacccacagc gccaccacgc agctggccct gacgaagacc ttcacgcgcg atgatataca
    35701 tgatctgatc gccatcgact ttattgcctt catggtcaac cataatcacg cgatcgccat
    35761 cgccgtcgaa ggcaataccg agatccgctt tttcagccag cacacgagcc tggagcgcgc
    35821 gaacgtcggt agcccccact tcggcattga tgtttacacc gtttggctca caaccgatag
    35881 cgataacgtt cgcccccagt tcgcgcagca cgttcggcgc gatgtgataa gtcgcaccgt
    35941 ttgcacaatc caccacaatc ttcagttcac tgaggctaag ttcgttcggg aacgtggctt
    36001 tgcaaaactc gatatagcga cccgcggcat caacgatacg gctggcttta cccagttctg
    36061 ccgaatcaac gcagctgatc tccttttcca tttccgcttc gatggcctct tctaccgcat
    36121 ccggcagttt ggtgccgtcg atagagaaga atttaatgcc attatcgtag aacgggttat
    36181 gcgatgcaga tatcacaatt ccggcctctg cgcggaaggt acgcgtcaga taagccacgg
    36241 ccggtgttgg catcgggcca gtgaagagtg cggaaaggcc cgctgccgcc agacccgctt
    36301 ccagtgctga ctccagcata tagccagaaa tacgcgtgtc tttaccaata ataatcttac
    36361 gggagccgtg gcgcgccagc actttacccg cggcccaacc cagcttaagc acaaaatcag
    36421 gtgtgatcgg cgcatcccct acacgaccac gaatcccatc ggtaccgaaa tatttacgat
    36481 tactcatagc gtttgttttc ctttgcagac agagtggctt ccaccacccg catcgcttct
    36541 acggtttctt tgacgtcatg aacacgaatg atgtgcgcgc cttgcattgc ggcaatgacc
    36601 gcacaggcca gactaccgct caggcgctcg gacggcccca cgttcagcag ctgcccaatc
    36661 atcgattttc gtgacatacc caccaacagc ggcaggttga aatggtgaaa ttcagccagg
    36721 cgcgccagta atgaatagtt atgggagaga tttttaccga aaccgaatcc ggggtcgagc
    36781 aacaatttct cttttgcgat acccgcctgc tcgcaacgtg ctatttgctc aataaagtag
    36841 cgattcactt ctgcaaagac atcgtcatac ttcggagctt cctgcatggt ttttggattt
    36901 ccctgcatat gcatcagaca aaccggtaaa ccggtttctg cagccgcctc cagagcgcca
    36961 ggttcggaaa gggagcggat atcattaata atgtgagcgc caactttcgc tgactcacgg
    37021 atgacttctg gtttggatgt atcgactgag atccagactt cgaagcgttg agcaattgcc
    37081 tcaaccacag gaataacacg ttgcaactct tcttcaacgc taacttccgc cgcccctggg
    37141 cgcgtggact cgccaccaac gtcaatgatc gtcgcgccag cgttgatcat cagattcgca
    37201 tgtttcaccg catctatcag cgagttatgc gtgccaccat ccgaaaagga atcaggcgtg
    37261 acgttgagga tccccattac gtgaggatgg ctaaggtcca gtgaagtacc ctgggcaaag
    37321 agtttcatga tgttatccct ggtatgaatt gataagaaaa aaccccggag cacgccccgg
    37381 ggttttcggt acaaatacag tcatctgatg cgggaactta cttgtcgcct aactgctctg
    37441 acatggtgtt acccgggttc ggcgtacgcg gttcatcaac cggacgagga gcctttggac
    37501 taccattgtc gccagaattg ttagaagcgc ctggttcttc ccagcccgct ggcggacgta
    37561 catcgcgacg tgccatcagg tcatcaatct gcggtgcgtc gatagtctca tatttcatga
    37621 gagcatcttt catcgcatgc agaatatcca tattgtcggt cagaagctga cgcgcacgat
    37681 tatagttacg ctcaatcagt gctttcactt cctggtcgat gatacgtgca gtttcatcgg
    37741 acatatgttt cgctttcgct acgctacggc cgaggaacac ttcaccttct tcttccgcgt
    37801 acagcagtgg acccaatttc tcagagaagc cccactgagt caccatgtta cgtgccaggt
    37861 tggtcgcaac tttaatatcg ttggacgcac cggtagatac atgttccggc ccgtagatga
    37921 tctcttctgc cagacgacca ccgtacagcg tagaaatctg gctttccagt ttctgacggc
    37981 tggcgctgat tgcgtcgccc tcaggcaaga agaaagtcac acccagcgca cgaccgcgtg
    38041 ggataatcgt cactttgtgc accggatcgt gttccggcac caggcgaccg ataatcgcat
    38101 gacccgcttc gtggtaagcc gtcgattctt tctgcgcttc cgtcatcacc atggagcgac
    38161 gttccgcacc catcatgatt ttgtctttcg ctttctcgaa ctcaaccatc gacacaacgc
    38221 gtttgttgcc acgagcagcg aacagtgccg cttcgttcac caggttcgcc aggtcagcac
    38281 cggagaaacc aggagtacca cgggcaatga ttgccgcgtc gatatcgggt gccaatggta
    38341 cgcgacgcat gtgaactttc aggatctgct cacgaccgcg aacatctggc aagccgacca
    38401 caacctgacg gtcgaaacgg ccaggacgca gcagggccgg gtcgagaacg tccggacggt
    38461 tagtcgcggc gataacgatg ataccttcgt taccttcgaa gccatccatc tcaaccagca
    38521 tctggttcag agtctgttca cgttcatcgt gaccaccgcc cagaccagcg ccacgctggc
    38581 ggcctacggc gtcgatttca tcgataaaga tgatgcacgg tgccgctttc ttcgcctgtt
    38641 cgaacatgtc acgaacacgg gatgcaccca caccgacgaa catttctacg aagtcagaac
    38701 cggagatagt aaagaacgga actttcgctt cgcctgcaat cgctttcgcc agcagcgttt
    38761 taccggtacc cggaggaccg accatcaaga cgcctttcgg gatcttaccg ccgagtttct
    38821 ggaagcggct cggctcgcgc agatactcaa ccagttcagc aacttcttct tttgcttcgt
    38881 cgcagcccgc aacgtcagca aaggtcgttt tgatctgatc ttccgtcagc atgcgcgctt
    38941 tgctcttacc aaacgacatg gcacctttgc caccgccgcc ctgcatttga cgcatgaaga
    39001 agatccagac accaatcagc aacagcatcg ggaaccagga gatgaagata gaagccagca
    39061 ggcttggttc ttcaggcggt tcaccgacaa ccttgacgtt cttggtcaac aggttatcca
    39121 gtaatttcgg atcctgaacc ggaatgtaag tggtataacg gttactatct ttcttggtaa
    39181 cgttgatttc acgtccgttg atacgcgctt cacgaacctg gtcgttattg acctcttgta
    39241 ggaaggtaga gtaatccacc ttacggccat tagactcgct gggcccaaag ctctggaata
    39301 ctgacatcag cacaacggca atgaccagcc agagtattag gtttttcgcc atgtcactca
    39361 agggattaac ctcttattac aactgtgtta aaaacagcgt caggatactc tatatccagc
    39421 atctttcaaa ctttcgtctg aaatctcccg gttagggttt acgcccggtc gctacaatat
    39481 acacttcccg cgaacgtgca cgagaagagt ccggcttacg aactttgacc ttcgtaaaca
    39541 gggagcgaat ttcccttaga tactcatcga aaccttcgcc ctggaacacc ttcactacaa
    39601 aactgccacc tggcgctaat acatcacgac acatttctag cgccagttcc accagataca
    39661 tggcacgggg gatatccacc gccggtgttc cgctcatgtt tggtgccata tcggacatga
    39721 caacctggac tttgctgtcg ccaacgcgct ccagcagtgc tttcatcacc agttcatcac
    39781 gaaaatcgcc ctgaagaaag tccacaccaa cgataggatc cataggtaaa agatcgcaag
    39841 cgatgatgcg gcctttgccg ccaatttggg tgaccacata ttgtgaccaa ccacccggag
    39901 cagcaccaag gtcgacaacc gtcattcccg gtttaaagag tttgtcactt tgctgtattt
    39961 catcaagttt aaaccaggca cgggaacgta accccttttt ctgtgcctgt tgaacatatt
    40021 tatcgctaaa gtgttcctga agccagcggc tggagctggc agaacgcttc ttacctgtca
    40081 tttaactttc ccatggggat aactcatcgt aaccaattgc gtaaattttt acgcgcctat
    40141 ttggtgatat atgggagatg gcggtagaat gacccgtttt caatcccaac gtaagcaaaa
    40201 aatacgatga atctgagtac taaacaaaaa cagcacctga aaggtctggc acatccgctc
    40261 aagccagttg ttctgcttgg cagtaatggt ttgaccgaag gggtgctggc cgagattgaa
    40321 caagcgttag agcaccatga actcatcaag gtgaaaatcg ccaccgaaga tcgcgaaact
    40381 aaaaccttga tcgtggaagc tatcgtgcgc gaaaccggcg cctgtaatgt acaggtcatc
    40441 ggtaaaacgc tggtgcttta tcgcccaact aaagaacgta aaatctcgct gccacgctaa
    40501 gattatccta aagttacaca catttgctgt gtaaaacgag gggttttccg caggcaggag
    40561 agcaaaatgc cacgctctgt tcgttgataa aaggccgcat agcggccttt ttcctttctt
    40621 tacaatacat caacatcttg agtattgggt aattcttaca ggtattccac cttaattact
    40681 tcaaattcta cttcgccgcc cggcgttttg atgaccacaa catcatcttc ttctttgccg
    40741 atcaggccac gagcaatagg cgagtttaca gaaatcaggt tttgtttaaa gtcagcttcg
    40801 tcatcgccaa cgatgcgata agtctgttct tcgtcagaat ccagattcag caccgttacg
    40861 gtagcaccaa aaataacgcg cccattgttg ggcattttgg tgacatcaat cacctgcgcg
    40921 tttgacagct tggcttcgat gtctttgata cggccttcgc agaagccctg ctgttcacga
    40981 gctgcgtggt attcggcgtt ttctttcagg tcgccatgct cacgcgcttc cgcgatagca
    41041 gcaatgattt caggacggcg cacagatttc agaaaatcca gctcttcgcg taatttttct
    41101 gcgccgcgta aggtcatcgg aatagcttgc atttgttata cctcttgaat attcctgatg
    41161 gggcaagtct tcacccaccc cggctgttcg gcctgcccgg cataactcct caccgggacc
    41221 agaagcaaaa aaataccgac ccgggtacaa gtcccaggtc agctacaatt cacattttga
    41281 tagtcatttt accctgaagt tcccgaaggg tcatcgttta ctttataggg cgttgcgccg
    41341 tagtatgacg gctcgattcc aggttgttag cgcgagatta tgcgattttc cagatttatc
    41401 atcggattga ccagctgtat agcgttcagt gttcaggccg caaatgttga tgagtacatt
    41461 actcaactcc ccgctggtgc caaccttgcc ctgatggtgc aaaaagtcgg cgcgtcggcc
    41521 cccgctattg attaccacag tcagcagatg gcgctgcctg ccagtaccca gaaagtgatt
    41581 actgcgctgg cggcgttgat tcaactcggc cccgattttc gttttaccac gacgcttgaa
    41641 accaaaggca atgtggaaaa cggcgtactt aagggtgact tagtggcgcg atttggtgcc
    41701 gatccgacgt taaaacgtca ggatattcgc aatatggtcg cgactttgaa aaaatctggc
    41761 gtcaaccaaa tcgatggcaa tgtgttgata gatacctcca ttttcgccag ccacgataaa
    41821 gcccccggct ggccatggaa tgacatgaca caatgcttta gcgctccgcc tgccgccgcc
    41881 atagttgacc gcaactgttt ctccgtctcg ctctacagtg ccccaaagcc tggtgatatg
    41941 gcttttatac gcgtggcatc ttattacccc gttacgatgt tcagccaggt acgcaccctc
    42001 ccccgtggtt ctgccgaagc gcaatactgc gaactggatg tggtgccagg cgacctgaac
    42061 cgctttacgc tgacgggatg cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc
    42121 gtgcaggatg gagccagcta tgccggtgca attctgaaag atgagttaaa acaggcgggt
    42181 atcacctgga gcggaacact gctgcgccag actcaggtta acgaacctgg aacggtagtt
    42241 gccagtaaac agtcggcccc gctgcacgat ctgcttaaga ttatgctgaa aaagtcggac
    42301 aacatgatcg ccgatacggt tttccgcatg ataggccatg cgcgcttcaa tgtgcctgga
    42361 acatggcggg ccgggtcgga cgccgtgcgt cagatcctgc gccagcaagc cggtgtcgat
    42421 attggaaaca ccattattgc cgatggttca gggctttcgc ggcataacct gattgccccc
    42481 gccaccatga tgcaggtgct gcaatacatt gcccaacacg acaatgaact taactttatc
    42541 tccatgctgc cgctggcggg ctatgacggc tctttgcagt accgtgcagg tctgcatcag
    42601 gcgggcgtgg atggaaaagt ctcagcgaaa accggttcgt tgcagggggt atataacctg
    42661 gcgggattca ttaccacagc gagcgggcaa cgaatggcgt ttgtgcaata tctttctggc
    42721 tatgcagtag aacctgcgga tcagcgtaat cgccgtattc cgttagtgcg ttttgaaagc
    42781 cgtttgtata aagatattta tcagaacaat tagtcaaaaa gaaaccccgg cacatggccg
    42841 gggcttcaga ttattgacaa agtgcgcttt gtttatgccg gatgcggcgt aaacgcctta
    42901 tccggcctac aaaatcgtgc aaattcaaca tattgcaatt ctcttgtagg cctgataagc
    42961 gtagcgcatc aggctgattt ggcgtttatc atcagtgatt aacgcttgta aatgaactca
    43021 acgccttctt cgtcgtcttc gtcccagttg tcatcccagt cttcatcatc ctcttcagca
    43081 atctcttcaa gctgctggcg atgataatca tcccacatga attcgacttt ctctggctgt
    43141 ttcgcttctt cagcctgcac gaccgggttt tcaatgataa aggtcatcac atcccagcag
    43201 agatctttca cgcccagtcc actcgccgca gagatcagat aatatttatc ttcccagccc
    43261 agcgcctcag cgatcgcttt cgctttctct tcggcttcta ccttatccag cagatcgatc
    43321 ttgttgaaca ctaaccaacg cggtttcgcc gccagatcct ggctgtattt ttccagctcg
    43381 ctgataataa tacgcgcgtt ttcaaccgga tcggtgccgt caatcggatc gatatcgatg
    43441 aggtgcaaca ggacgcggca acgttccagg tgcttcagga agcgaatgcc cagacctgcg
    43501 ccttccgcag cgccttcaat cagtcctgga atatcggcaa caacgaagct cttttcgttg
    43561 tccattcgta ccacacccag acttggcacc agagtggtaa acggataatc cgccactttc
    43621 ggtttagccg ccgataccgc acgaataaag gtcgatttac ccgcgtttgg catccccaac
    43681 ataccgacgt cagccagcag catcagctcc agcagcagct cgcgcttatc gcccggcgtg
    43741 ccgttggttt tctgccgcgg tgtacggtta acggacgatt tgaaacgggt attgcccaga
    43801 ccgtgccagc cgcccttagc aaccagcaga cgctgaccgt gtttggtcat atcgcccatg
    43861 gtttcaccag taccctggtc gattacacgc gtacctaccg gcactttaat tgtgacgtct
    43921 ttaccgcgct taccggtaca gtcgcggctt gcgccgttct gaccgcgctc tgcacggaaa
    43981 gatttttcaa aacgataatc gataagcgtg ttcaggttct cgtcggcttc catccatacg
    44041 tcaccaccat caccgccgtc gccgccatcc gggccgcctt tcggaatata cttttcgcgg
    44101 cggaagctca cgcaaccatt accgccatca cctgcaacga ccagaatcga tgcttcatca
    44161 acaaacttca ttttattctc cgtaaatcat tcgcctgcgc ggggttgcga taccaccgtt
    44221 gtatgcttac gtaatccgcc ccaaatacga tgaccaatgg cggaatacat cgcgcccgca
    44281 accacgacaa acgcaccgag ataacctaaa aggtttaaca tcggtctggc gaagaaatcg
    44341 ggccaggcca gtgataaaag atctgaaaaa aacagcgtaa acagtggggt gagcgtgatg
    44401 atcgcgctca cctgcgctgc ctgccagcga gccatcgctt ccgccagggc gccatatcct
    44461 accaatgtat tcagtccgca aaaaattaaa catgcgagct gccagtggct aagctgcgct
    44521 atcactccag gctttgccag agggaagagc gcaattgtac ataaagtgta cagtaaaaac
    44581 aggatctgcg gtgaggccag ccgacgcaat aaaacctttt gcgccacgcc ataactcacc
    44641 caaaccgtcg cagcaccgac cccaaagata actccccagg tgtaatcggt gagctttgta
    44701 aatatctcga ccagactggt gttaaaaaac atcaccaggc cgctcaggag catcaatgcc
    44761 cctacaacct gagtgctgcg cattttctct ttcaggataa atacgctggc aaccatcatg
    44821 ccaactggcg agagttgccc aatcacctgc gaagcggtcg gactcaggta ttgcaaggat
    44881 gagctgaaca ggatgaagtt cccaaacagc ccggcggtcg ccactgccaa caaaatcaac
    44941 cagcgtggct tacgaaacac gcgtaatggc ggcaacctct tcttcaccgc aagaatggca
    45001 cccaggccaa tactcgccat caagaaacgg taaaacacga ttgtcggagg ttccatcacc
    45061 tccagcacct gcttcattgc gattggcaac gccccccagc aaattgctgt ggtgagcgcc
    45121 aaaagaatgc caatgcctgc ctgctgcttc ataccgtatt ccctgcaaag cgcatttgcg
    45181 gattaacgtc gcaccatgcg catttttacc ggttatcgaa tgtaaaaagc cccgcaacgt
    45241 gttgcggggc tttcatccgt taccgggacg cgaaaaactt attcagcttc gatgctgata
    45301 aatttacggt ttttcgggcc tttaacttcg aatttcactt taccgtctgc tttagcaaac
    45361 agagtgtggt cacgaccgca acctacgtta gcgccagcgt ggaatttggt accacgttga
    45421 cgaacgatga tgctacccgc cagaacggat tcgccaccga aacgcttaac gcccaagcgt
    45481 ttagcttctg aatcgcgacc gttacgtgtg gagccgccag cctttttatg tgccatttga
    45541 aatctctcct caggtcttag gcgctgatgc cagtaatttt cacatcagtg aaccactgac
    45601 gatggccctg ctgcttacga tagtgtttac gacgacgaaa cttaacgatt ttaactttct
    45661 cgccacgacc gtgagcaaca acttcagctt tgattacgcc gccatcaacg aaaggaacgc
    45721 cgattttgac ttcttcaccg tttgcgatca tcagcacttc agcgaactca acagtttcgc
    45781 cagttgcgat gtccagcttt tccaggcgaa cggtctgacc ttcgcttact cggtgttgtt
    45841 taccaccact ttggaaaacc gcgtacatat aaaactccgc ttccgcgcac accttttcaa
    45901 tgattcagag tgcgctataa atattcacaa tagggcgcga atattacgca aaacgcacgc
    45961 ctttgacaag tgctacagtc aatacacgaa gaaaaaaaac acaacttgta cggtaacgtt
    46021 tatctgtgcc attttttcag tacaatcacc ctatattcct aaccataaac cctaagttgc
    46081 ctttgttcac agtaaggtaa tcggggcgaa aagcccggct tttgcgatga atttagaaaa
    46141 aatcaatgag ttaaccgcgc aagatatggc gggtgttaat gcggcaatcc ttgagcagct
    46201 taattccgac gtccaactga tcaatcagtt aggctattac atcgtcagcg gcggcggtaa
    46261 acgtattcgt ccgatgattg ctgtactggc tgcacgagct gttggctatg agggaaatgc
    46321 gcatgtcacc attgctgccc tgatcgagtt tatccacacg gcgactctgc tgcacgacga
    46381 cgttgtggat gaatcagata tgcgcagggg taaagctacc gccaacgccg catttggcaa
    46441 tgccgccagc gtgctggtag gcgattttat ttatacccgc gctttccaga tgatgaccag
    46501 cctcggttca ctcaaagtgc tggaagtcat gtcagaagcc gtaaacgtca tcgcagaagg
    46561 tgaagttttg caactgatga atgttaacga tccggacatc actgaagaaa actacatgcg
    46621 cgttatctat agcaaaaccg cgcgtctgtt tgaggctgcc gcgcagtgtt ccggaattct
    46681 ggctggctgt acgccggagg aggagaaagg cctgcaggat tatgggcgct atctcggcac
    46741 tgctttccag ttgatcgacg atttactcga ttacaatgcc gatggcgaac agttaggtaa
    46801 aaacgtcggc gacgatctga acgaaggtaa accgacgctg ccgctgctgc atgcgatgca
    46861 tcatggaaca ccagaacagg cacagatgat ccgtaccgcc atcgaacagg gtaacggtcg
    46921 ccatcttctg gaaccggttc tggaagcaat gaacgcttgt ggatctcttg aatggacgcg
    46981 tcagcgcgcc gaggaagaag cagacaaagc catcgcagcg ttacaggtgc tcccggacac
    47041 cccttggcga gaagcactca tcggcctcgc gcacatcgct gttcaacgcg atcgttaatc
    47101 ccctcccctc atcccgcgca gtgcgcggga tgattccagt aaactccata agaatactta
    47161 ttcagcgtaa taatgcactg aaaaaccagt gtatacatct ttatatattc tgaatattca
    47221 cactctttac aggaactttt tagagcaatc ggccatcagg agtatagtga tgctcgacag
    47281 aagaagtgtt ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt
    47341 cattgactgg catcccgctg acatcattgc gggtttgcgc aagaagggaa cgtcaatggc
    47401 ggcggaatct cgcagaaatg gtttgagttc ctcaacgctg gcgaatgcat tatcgcgccc
    47461 atggccgaaa ggagagatga ttattgcgaa ggccctggga actgacccct gggttatctg
    47521 gccatcacgc taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg
    47581 tagctacact aaaccgaaaa aatgagtggt ctggcggtag ccccgcgtac ggggctgcca
    47641 gctttcagac gattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgcagttt
    47701 gtcttcaatg cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc
    47761 ttccgcaata cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac
    47821 ctgtgcgcca gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc
    47881 gtgcgcgccc atacggctca gctctggcac atgcataaag cggttttcaa agaccgtttc
    47941 ggtgatgaat ccagtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc
    48001 ggtcgggaat gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg
    48061 catatccagg ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt
    48121 cgccagcacg gcgtcgagag tatctggctg cgcgttacgg cagataattt tgccgcgaga
    48181 aatcgccgcc gccaccagga atgtaccggt ttcgatacga tccggcagca cgcgatagac
    48241 accgccgcct aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat
    48301 tttcgcaccc agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc
    48361 gttttcaata atcgtggtac cttccgccag ggttgcagca cacatgatgg tcaccgttgc
    48421 gccaacgctg actttatcca tcacgatatg tgcgcctttc agacgaccat cgacggaagc
    48481 tttaacgtaa ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg
    48541 tagatcaacc ggacgcgcac cgatcgtaca accgccaggc agtgaaactt gcccctgacc
    48601 aaagcgcgct accagcggcc ccagtgccca gatagaagca cgcatggttt taaccagatc
    48661 gtaaggtgcg cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc
    48721 tactttcgca cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagtttcgg
    48781 gacgttctgg atctctaccg gttcttccgc cagtagtgcg gcaaaaagga taggcagagc
    48841 agcattttta gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg
    48901 aaatttatcc atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc
    48961 catagctcaa aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt
    49021 aatcgacaca gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt
    49081 ctgctgtttt ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa
    49141 gtggctgcca tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct
    49201 ctgaatttca ttattttcca tgggatcttc aatcatcagt taataaacca gcgaaacatc
    49261 ttagagcaaa gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg
    49321 ctcgacttgc aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat
    49381 ttaacgaggc agaacatcag caggcaaatt atacaatttc gccagggtat acactttgtc
    49441 gtttaccccc tgaagcgtca cattgttgcc ctgctttttc gccagatcga taagatggag
    49501 cagcagtgcc agtccccccg tatccacgcg ggagacacgg ctaagatcga tgcaggtaat
    49561 ccccttcacc gcttcctcac gcatttccca aagcggtagc aaaacgtcct gatccagctc
    49621 tccggataac gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt
    49681 ttctcttcca gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg
    49741 atacctttgg tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg
    49801 ccttcagcaa tcatgtcgta agcctgccaa ttgcccgtct gggagttttt acgccactgg
    49861 aagtccagac gcaccggcgg acggccattc gggtcaataa tggtaacgcg aataggcaca
    49921 atggttttat cgcccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc
    49981 gccagcgcct gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaggcttca
    50041 cgttgagcag gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc
    50101 acctgtacgt atggcagcag ttcctgatca acaatggtac gcagataatc cgggttggcc
    50161 cgaatttgcg gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc
    50221 agcttatacg gattggtctg gtctgccgcg gttgccgcac tcagaggtgc aatcaccagc
    50281 aaagcgacca tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta
    50341 cccacaggtt cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc
    50401 ttattgtcat cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc
    50461 gcagacttag tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg
    50521 tcttcaaaac cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg
    50581 ctcagcgaac tggtatctgg aatgtggtta taacgttgtt caatttccag cgttacgcgc
    50641 ggcagatagg ttttcgggtc cagcgtaata tccgccaccc gacccacaac aacgccacca
    50701 atactgaccg gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg
    50761 taggtcggtt cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc
    50821 gccagcagtg ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg
    50881 aactcaattc ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga
    50941 cgagtggaca acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc
    51001 gtagccgtta aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag
    51061 acagttgacc agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc
    51121 gctatcaatg cctttccaac tgacgccgac cagagatccg ccccagatcc ccacggcgac
    51181 gaaaataacc gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg gagaaataac
    51241 ccgacgcagc ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat
    51301 caggccgatt tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac
    51361 caccggcccc agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc
    51421 actataagtg gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc
    51481 agaaaccaca ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag
    51541 cggcgcatgt ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc
    51601 ccgcccgaac gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa
    51661 cagcatgagt ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc
    51721 ggaacggaac aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat
    51781 tcgcctgcaa cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg
    51841 cgtgatccgc aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc
    51901 ccagcgcgct gttcagctca gaaatcagct tcaccagtac gcccatggtg atgggatctt
    51961 gcccaacaaa aggttcatca aacatgatga gatccggctc cagcgcaatc gcacgcgcca
    52021 gcgctgcacg ccgcgccatc ccaccggaaa gttcagaagg cattagttta gccgctccac
    52081 gcagccccac ggcctccagc ttcatcatca ccgtactatg caacaatggc gcgggaagtt
    52141 gggtatgttc gcgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg
    52201 ccccggactg aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca
    52261 tcgccggaat attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc
    52321 caatcagacg gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct
    52381 tccctcgcgg cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac
    52441 tgacatcgcg catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt
    52501 gcgttgattt tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata
    52561 ttacccgccg tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa
    52621 aattaagcat ccgttacggc tttctgaaaa tcttcagcgg accggcgagt atacctgaag
    52681 aaaggacgtt agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt
    52741 tacagtgccg accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg
    52801 ccgctgatca tcggcatgac ggtggtcagt attggtacat cgttaccaga agtcatcgtc
    52861 tcgcttgccg cgtctctgca cgaacaacgc gatttagtcg ttggtacagc cctcggctca
    52921 aacattatca atatattgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc
    52981 cattctgatg ttctacgccg tgaattaccc ttaatgttgt tggtcagcgt ggtggccggt
    53041 tccgtactct atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct
    53101 gtgctatggc tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac
    53161 agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt
    53221 ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac
    53281 gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt
    53341 gctatcggaa ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa
    53401 aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg
    53461 ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag tcgtgactac
    53521 agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa
    53581 ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg
    53641 tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt
    53701 acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg
    53761 cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt
    53821 ctggtgtaaa gggaaagttg tcgtcatggg gatgggaaaa tcggggcata ttgggcgaaa
    53881 aatggcggca acgtttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc
    53941 cgcgcatggt gatttaggca tggttacccc acaggatgtg gtgattgcta tctctaactc
    54001 tggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt
    54061 aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg
    54121 tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc
    54181 cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc
    54241 tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt
    54301 aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg
    54361 tgacgcgttg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa
    54421 tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt
    54481 ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc
    54541 tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat
    54601 ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac tgcgtgcagg
    54661 cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc
    54721 tgttacggcc ctgtcagcgc cgacgttata gcaaaagcag agaacattcg tctgctgatc
    54781 ctcgatgtcg atggcgtact gtcagatggc ctgatttata tgggcaataa tggcgaagag
    54841 ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt
    54901 gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg
    54961 gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag cgatctgctg
    55021 gaaaaactgg cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg
    55081 ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc
    55141 ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc
    55201 gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta
    55261 aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcatta
    55321 atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata
    55381 aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga
    55441 ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac
    55501 ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc
    55561 tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca cttgtgccag
    55621 actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg
    55681 ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa
    55741 tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg
    55801 aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc
    55861 agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac
    55921 cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc
    55981 cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa
    56041 ggtaaagaag tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac
    56101 ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc
    56161 gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc
    56221 acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaaca
    56281 accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac cccggcacag
    56341 aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat
    56401 aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt
    56461 ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt aggcattgtg
    56521 ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat
    56581 gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt ccgtcgcctc
    56641 agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa
    56701 cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc
    56761 atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct
    56821 gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt
    56881 atcgacatta aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt gctgatcact
    56941 gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg
    57001 catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta
    57061 taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga
    57121 gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga
    57181 cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg
    57241 agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact catgaagaaa
    57301 tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa
    57361 aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta
    57421 caccatccgg caccagcggt gactacattg acgacgagct gccggtctac cagggcgaaa
    57481 cgacgcagac cttgcaggat tacctgatgt ggcaggttga gctgacaccg ttttccgaca
    57541 ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt tatctgactg
    57601 tcccgctgga agatattctc gaaagtatag gcgatgaaga gatcgacatc gacgaagttg
    57661 aagccgtcct taagcggatc caacggtttg atccggtcgg tgtggcggca aaagatctgc
    57721 gtgactgtct gctgatccaa ctctcccaat tcgataagac cacgccgtgg ctggaagagg
    57781 ccagactgat cattagcgat catctcgatc tgttagccaa tcacgacttc cgcactttaa
    57841 tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc
    57901 tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg
    57961 tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc
    58021 gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca
    58081 gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc
    58141 gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg
    58201 agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag gctgtcgaaa
    58261 tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca
    58321 tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc ggcgaagctt
    58381 cctccacggc gattcgtgcg ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac
    58441 cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc
    58501 gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacaac
    58561 tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc
    58621 gagatcaccg aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat
    58681 tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca
    58741 gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg
    58801 tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat
    58861 aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg
    58921 cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg
    58981 ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac
    59041 agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc
    59101 agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga agctatcctg
    59161 acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa
    59221 ctggaagaag atactctgcg cgccgttggc gttttcgttc agctcgaaac gcctatagct
    59281 ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt gccggcagac
    59341 caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc ggacaaaacc
    59401 atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat
    59461 accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag
    59521 gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc
    59581 gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg
    59641 ttacccgatc tggctcgaac cctggccgat cgcgagattt ctgccgccgt cagcattgat
    59701 gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac
    59761 gctttctcac cgcaactcct gttcctggat gccgaccgta ataccttaat tcgtcgttac
    59821 agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc
    59881 gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc
    59941 tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct gggtaaacgt
    60001 gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat
    60061 gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt
    60121 ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac
    60181 aattttatct accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac
    60241 aaccgtagct acttgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat
    60301 attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat
    60361 cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc
    60421 tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg
    60481 aagtgctctt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt attgcgctgc
    60541 tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt ccacaggaag
    60601 aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa gattaatctt
    60661 catcactttg acatacaact cccttcaaac tccccccgac aataagaaaa tcacgtactg
    60721 aaatcgttct caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc
    60781 ttaagaaaac cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag
    60841 atcgtcagaa aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg
    60901 gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga
    60961 ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg
    61021 cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg
    61081 agacggattg cggaaccggt tatgtctacg atgtgattgc agattttgac ggcaagcctt
    61141 ccattacgct gaccgaattt gctgaacaat gccgttacga agaagacatt gcacaactgc
    61201 gccagctact gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc
    61261 tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct
    61321 gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc
    61381 tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc
    61441 tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt
    61501 aatccagctg gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa
    61561 tccacgcctg acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat
    61621 taggtaatac agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt
    61681 ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat
    61741 tcaggtaaac cgtcaggata cgctttttgc tccagaccgt ttctatcccc agcgttaatc
    61801 cggcttccag cccttttcga acccagctac gcccatccca taaaaagaga tttttggctg
    61861 tctgttgaga aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca
    61921 gggctttctc aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg
    61981 cggcaatcac cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat
    62041 gtgccacgta acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg
    62101 ctgagaaggg aacaggcgca acgctaaaca acgcgatgcc cccgccccag aaaacggcga
    62161 ggacaaccat taaccgcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt
    62221 tactcattca gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc
    62281 tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac
    62341 cacgatatca tcgacaggac acggcacatg ctccgcgccc atctcttcca gcacttctgc
    62401 ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat
    62461 cgccggggca atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag
    62521 cgcctttaat tcacggtcaa cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt
    62581 cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg
    62641 ggccagagga cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt
    62701 ttccgtcatc gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc
    62761 aaagcagacc gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg
    62821 aatttcagaa ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt
    62881 gatcatcctt tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc
    62941 acacatttaa ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg
    63001 cctgaaaaac agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat
    63061 ttccctggtg ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg
    63121 cttttgccac ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt
    63181 caatccattc accgacgtta tcttcccagg ccggaaggtc aggagactga atttgctgac
    63241 ccagctgttg cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt
    63301 cctcaacaat gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc
    63361 ccggcatcat cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt
    63421 cgagatactg ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg
    63481 tcgtcaccgt actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta
    63541 gcgccggaac agaaagcggc ttactcagca catcatccat tccagcattg aggtactctt
    63601 gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat
    63661 aacgtttcgt cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca
    63721 gcaacaccag gtcgtattcg cccggtttaa acatctccag cgccgccttg ccggtcatgg
    63781 cgacatcaac gctgttacct aatttttcca gcacagaacg cgcaacaatc acgttcagtt
    63841 caatgtcttc caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg
    63901 catcatcgac ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt
    63961 tgccctgttc gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa
    64021 cggccagacc aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt
    64081 aatacatggc gaaaatttta tccagctcat cctgcggaat gccgatacca gagtcttcca
    64141 cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct
    64201 gggtgaattt gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt
    64261 cggtaatgac ctgatgcggt aatggcagcg tcggctccag gttaaagcgc aatccttttt
    64321 gttgcgcctg caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg
    64381 gttgattatc aagctggacc ttgcgtcgtt ccatcttatc catgtcgata atatcgttaa
    64441 agatattccc cagcgtgacg gccgaaacat ggatggtctt gagatatttt tcctgctcgg
    64501 cggtgagttc ggtatccagc agaatgcggc tcagaccgac gataccgttc agcggtgtac
    64561 gcaattcgtg actgatggtg gagataaacg tcgttttgtc gcggctggcc cgttcaagcg
    64621 catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt
    64681 tacccacgcg gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg
    64741 ggtaatccag ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg
    64801 tttcaatgac ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca
    64861 gttgtttttc gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt
    64921 tatcttcgtt acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg
    64981 aggattgctg ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt
    65041 ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg aacggcaatt tcctgattta
    65101 gctgggcaat attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct
    65161 ccagtttttg caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca
    65221 ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata gaacgaataa
    65281 catcaatgct ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg
    65341 caagaacgac gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca
    65401 tcaggtcaac ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg
    65461 acaacctgcc ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc
    65521 ggaattcccc tcaacgacct actccgttgg aggtagccac ggacgtccca gcgccgatcc
    65581 ctgtacgcca tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc
    65641 gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat
    65701 cacttccggt ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc
    65761 aataccgttc aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg
    65821 cagcggatgc agctttatgc cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa
    65881 acattcagcc tgcccttcgc caggcaggcc gacaattaag tgggaacaca ccttcagccc
    65941 gcgctgacgt gccagctggg ttgtacgctg ataacaggca aaatcatggc cgcggttgat
    66001 gcgatgcagt gttttgtcgt gggcggtttg tagccccagc tccagccaca cttcgtaacc
    66061 ctggtcctta tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgggt
    66121 acccacacac aaaccgacaa tattggcctg gctcaccgcc tgctgataca tagaacgcag
    66181 cacctgaact tccgcaaagg tgctggtata cgcctgaaag taggccagat agcgtttagc
    66241 gcggttaact aaattcgcct ggtgcgccag ttgctcggca atggaacgat gctgctgcgc
    66301 ttcatcggca aacgaggcaa cattacagaa tgtgcagcca ccacgcccga tggtaccgtc
    66361 acggttaggg cagctaaaac cgccgtgcag cgtcagctta tgcacctttt gcccataacg
    66421 acgggtgaga tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac
    66481 cgcgccttga aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat
    66541 caatcgtccc ggcctgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg
    66601 cataatcaca taaatcactt ttgcttatct tgtgtcagat ttttttatct cctgatggat
    66661 tttaggcaaa aacagtagca tgaaacgtca ttaccaatta aggcagtata aaatgctggt
    66721 tttgtcgtca gttcaaggca ggataagggt taacacacct ttatgacagt caggaattga
    66781 ctgtttctct aacgacttcc cttttagcct taaagataaa atccatttta atttcagtca
    66841 tttaataaag aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct
    66901 gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct
    66961 catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga
    67021 caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt
    67081 ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg gaggttcacg
    67141 atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca
    67201 tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc
    67261 agcaccgtgg cgcgattctc gccgatggta aaaccggcga cggttgcggc ttgctgttac
    67321 aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa
    67381 actacgctgt cgggatgctc ttcctgaata aagatcctga actcgccgct gccgcacgcc
    67441 gcatcgttga agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc
    67501 ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa
    67561 tttttgtgaa cgccccggca ggctggcgtc cacgcgatat ggagcgccgt ctgtttatcg
    67621 cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt
    67681 cgaatctggt gaacatctat aaaggtctgt gtatgccgac ggatctgccg cgcttttatc
    67741 tggatcttgc ggacctgcgt ctggaatcgg ccatttgcct gttccaccag cgcttctcca
    67801 ctaacaccgt accgcgctgg ccgctggcgc aaccgttccg ctatctggcg cataacggtg
    67861 aaatcaacac catcaccggt aaccgccaat gggcgcgtgc gcgtacttat aaattccaga
    67921 caccgcttat ccctgacctg cacgacgccg caccgttcgt caacgaaacc ggctctgact
    67981 ccagttcgat ggataacatg ctggaactgc tgctggcagg cgggatggat atcatccgcg
    68041 ccatgcgtct attagtacca cccgcctggc agaacaaccc ggatatggac ccggaactgc
    68101 gtgccttctt tgactttaac tccatgcata tggagccgtg ggatggcccg gcgggcatcg
    68161 tgatgtccga cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc
    68221 gctacgtcat caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt
    68281 accagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggttatcg
    68341 acacccgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc
    68401 atccatataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc
    68461 ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac
    68521 agtttaacta cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc
    68581 aggaagcggt cggttcgatg ggcgatgata ccccattcgc agtgctctcc agtcagccgc
    68641 gcattattta cgactacttc cgccagcagt ttgcccaggt gactaacccg ccaatcgacc
    68701 cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct
    68761 tttgcgaagc agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact
    68821 ccgatttcaa acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata
    68881 tcacctttga cgtcactaaa accacgctcg aagcgacagt caaagagctg tgcgacaaag
    68941 ccgaaaaaat ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta
    69001 aagatcgcct gccggttcca gccccgatgg cggttggcgc gatccagacc cgtctggtcg
    69061 atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc
    69121 accacttcgc cgtgttgctg ggcttcggcg cgacggctat ttatccatac cttgcctatg
    69181 aaacgctggg ccgcctggta gacacccatg cgattgccaa agattatcgt accgtgatgc
    69241 tcaactaccg taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct
    69301 ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg
    69361 tagtgggcct gtgcttccag ggggcggtca gccgcattgg tggagcaagc tttgaagact
    69421 tccagcagga tctgctgaat ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc
    69481 agggcggtct gctgaaatac gtccacggcg gcgaatacca cgcctacaac ccggacgtgg
    69541 tgcgcacgct gcaacaagcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga
    69601 agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg
    69661 aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa cgctttgata
    69721 ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga
    69781 tgaacagcat cggcggtaat tcgaactccg gtgaaggcgg cgaagacccg gcgcgctacg
    69841 gcaccaacaa agtgtcgcgc atcaagcagg tggcttccgg tcgctttggg gttactccgg
    69901 cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccaggcg
    69961 aaggcggtca gttgccgggt gataaagtca cgccttacat cgccaaactg cgctattcgg
    70021 tgcccggagt gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaggact
    70081 tagcgcagct cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc
    70141 tggtttccga accgggagta ggcaccatcg cgactggcgt ggcaaaagct tatgcggact
    70201 tgatcaccat cgcaggctat gacggcggca ccggcgcaag tccgctttca tcggtgaaat
    70261 acgcaggctg tccgtgggag ctggggcttg ttgaaaccca gcaggcgctg gttgctaacg
    70321 gcttgcgtca caagatccgt ttgcaggtcg atggcggcct gaaaacgggt gtcgatatca
    70381 tcaaggcggc gattctcggc gcagaaagct tcggcttcgg cactggcccg atggtggcgc
    70441 tcggctgtaa atatctacgt atttgccatc tgaacaactg cgcaacgggt gtagcaactc
    70501 aggatgacaa actgcgtaag aaccactatc acggcctgcc attcaaggtg acgaattact
    70561 ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca gcttggcgta acacgtctgg
    70621 tggatctgat tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc
    70681 agaagctggc gctgtcgaag ctgctggaga ctgccgaacc gcatccaggt aaggcactct
    70741 actgcaccga aaacaacccg ccgtttgata acggcctgct gaacgcgcag ttgctgcaac
    70801 aggcgaaacc gtttgtcgat gagcgccaga gcaaaacctt ctggttcgat attcgcaaca
    70861 ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg
    70921 ggctggcagc cgatcctatc aaagcgtact tcaacggcac cgcaggccag agcttcggcg
    70981 tgtggaacgc gggcggcgtg gaactgtacc tgaccggtga tgccaacgac tatgtcggta
    71041 aaggcatggc gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc
    71101 atgaagcaag cattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg
    71161 ccgcaggccg cgcgggtgaa cgtttcggcg tgcgtaactc cggtgctatc accgtggtag
    71221 aaggcattgg cgacaacggt tgtgaatata tgacgggtgg tatcgtctgc attctgggta
    71281 aaaccggcgt taacttcggt gcgggcatga ccggcggttt cgcttacgtt ctcgatgaaa
    71341 gcggcgattt ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgctc
    71401 tggcgatcca tgaagagcat ctgcgtggtc ttatcaccga gcatgtgcag cataccggct
    71461 ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg
    71521 ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca ccgtagtcgt agcgcagctg
    71581 agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta
    71641 tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg
    71701 ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt
    71761 cgtgcggcaa cccatactgc gagtggaaat gcccggtaca caactacatc ccgaactggc
    71821 tgaagctcgc caacgagggg cgtatttttg aagcggcgga actgtcgcac cagaccaaca
    71881 ccctgccgga agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca
    71941 ctctgaacga tgagtttggc gcggtgacca tcggcaacat tgagcgctat atcaacgata
    72001 aagcgttcga gatgggctgg cgtccggata tgtctggtgt gaaacagacc ggtaaaaaag
    72061 tggcgattat cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg
    72121 gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta
    72181 ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca
    72241 tgggtattga attcaaactc aataccgaag tgggccgcga cgtacagctg gacgatctgc
    72301 tgagtgatta cgatgccgtg ttccttggcg tcgggactta tcagtcaatg cgcggcgggc
    72361 tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttcctcatc gccaacacca
    72421 aacagttaat gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac
    72481 gcgtggtggt ccttggcggt ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc
    72541 agggagcgaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc
    72601 gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccgc
    72661 tgggtattga agtgaacggt aacggcaaag tcagcggcgt aaaaatggtg cgtaccgaaa
    72721 tgggcgaacc ggacgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata
    72781 tcgttccggc agatgcggtg atcatggcgt ttggtttccg tccacacaac atggaatggc
    72841 tggcaaaaca cagcgtcgag ctggattcac aaggccgcat catcgccccg gaaggcagcg
    72901 acaacgcctt ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt
    72961 ccgatctggt ggtgaccgct attgccgaag gtcgtaaggc ggcagacggt attatgaact
    73021 ggctggaagt ttaagcgagg taacaatgaa ttcgccagac tgtgtctggc gaatcaaatt
    73081 actgacgaag tgcgcgttgt tttctggcaa atgcggcatg agcgtttgac ctttcctata
    73141 atagggataa ttttcaataa attgtgcttt gttcaccaag tctgatgagt gaagcacatc
    73201 aagcaatttc tcatttacaa tccgtctcaa ttacttgtta acacctctga caataccgat
    73261 tctgcaagct attcagtctt ttcattccat gtaaattgtc tcgatattaa tatacaaaat
    73321 atgaatataa aaaaccaata tattatcctt aattatctat atattttcct atttttcagc
    73381 gcaaacattc atgtaatgaa taattatcac cacaaaccac ctcaccagca ccttattatt
    73441 cacatgatcg taactataaa attcattaaa taaatacatc ccagcaagtt ggctatttct
    73501 gaacgagtta tttatttaca ttcacaaaca gatatatata ctgcttcagc tttttatttt
    73561 aaaaaataaa tttgttatta aggatatgtt catatgtttt tcaaaaagaa cctcacaaca
    73621 gctgctattt gtgcagcact ttctgttgca gcattcagtg ccatggcaac agattcaact
    73681 gatacagaat taactatcat cggcgagtat acccctggtg catgtacccc agttgttacg
    73741 ggcggcggca ttgttgacta tgggaaacat cataacagtg cgctgaatcc gaccggtaaa
    73801 agtaataaac tggttcaact gggccgcaaa aacagcacat taaatataac ctgcacagca
    73861 ccaacgctaa ttgctgtaac ctccaaggat aaccgtcagt cgaccattgt agcgttaaac
    73921 gacacatcct atattgaaaa agcttatgat actctcgtag atatgaaggg aacgaaaaac
    73981 gcctttggtt taggttctgc gcctaatgga caaaaaattg gcgctgcatc cattggtatt
    74041 gacaggtcta atggtgggat tcacgcggcg gacgatacag gtgaaattcc tgtcgacctg
    74101 attcaaactg accactggtc agcagcgacc cccacatgga aagcctcatc taatggcgca
    74161 ttctgctcgc tgacaagttg ctcagcaatc gagcgcggtt attctgttgc aaaaacaggg
    74221 gaactgacac ctgttgccat tacagcggtc accttcccgt tactgattga tgctgcggta
    74281 aatgacaata ctattcttgg ttctgatgaa acgatcaaac tggatggtaa cgtcactatc
    74341 agcgttcagt atttataaga acttgtaata cagtttttat ttttacgcat taaccagtta
    74401 gctataactc ggacagatta tgccctatga catggtctgt cttaatcaat ctcatcgcaa
    74461 acagtgcata acatatatta acaatatgtt tctgcgttat ttatcattat tacaggagtt
    74521 ttaatttaaa tgctcagaca cattacattc actgtattta taacaacatc aatgaatacc
    74581 ttagccacag ggatggttcc tgaaacatca gtattgctgg ttgatgaaaa gcgtggtgaa
    74641 gctagtatta atattaagaa tacagatgat catccttcat tgctttacac caccattgtt
    74701 gatttgcctg aaagcaataa atcaattcgt ttaatcccga ctcagcccgt tattcgtgtt
    74761 gaagccggtc aagtacaaca ggttcgattt ttacttcagg cgactgttcc tctgcaatca
    74821 gaagagctga aacgcgtaac gttcgaaggt attccaccaa aagatgacaa gagcagcaga
    74881 gttactgttt caattcgtca ggatctacct gtactgatcc acccggcttc cctgccggaa
    74941 gagcgagaga cctggaaatt cctggaatgg cgtaaaaatg gcgaccaaat tgagattagc
    75001 aaccccagta attatgtcgt tcgtatgacc ttacaattta aaacgctccc ttcaggaaaa
    75061 acgggggcta taaataagac ctattttcta ccacacacca gtacaactac tgcattaaca
    75121 aacgctacgg atacaaaagt cgagttttac ccagccagcc gatatggtta tcgcggtaat
    75181 aaatatgtca ctgacttaaa ataactttgc ctggagcgac aaggatgtta aaaaaaacgt
    75241 tactggccta caccattggt tttgcctttt ctcccccagc caatgcagat ggtatagaga
    75301 ttgccgctgt tgattttgat cgggaaacat taaaatcact aggtgtagat cctaatatat
    75361 cgcattattt ttcccgttca gcccgttttt tgccaggtga atattcactg atagtatcag
    75421 taaatggcga aaaaaaaggc aacattgcta cgcgctttga tgaaaatggc gacatttgtc
    75481 ttgatcaggc atttctgcaa caagccggtt taaaaattcc ttctgaagaa aaaaatggct
    75541 gttacgacta tatattgtcc tacccgggta ccacaatcac accattacct aaccaggaag
    75601 cgttagatat tatcgtttca ccacaggcga tcattcccat agggttggat ctcacaaacg
    75661 cagcaactgg tggaacagct gcgctgctaa actactctct gatgagcagc cgtgcagaat
    75721 tttctaatgg gagttcggac tactcccagg ctgcacttga aggcgggatt aatattaatg
    75781 actggatgtt acgcagccat cagttcctta cacaaacaaa tggcacattc agtaaccaga
    75841 actcgtcaac ctaccttcaa cgtaccttta cagatcttaa aacactcatg cgagcaggtg
    75901 aagttaacct caataatagc gtgttggaag gagccagtat ttacggtatc gaaatcgcac
    75961 cggacaacgc attgcaaacc agcggcagtg gtgtgcaagt tactggtata gccaacacct
    76021 ctcaggctcg tgtcgagatt cgtcaacaag gagttttaat tcattccatt ctggttcctg
    76081 cgggcgcatt cactatccct gatgtacctg ttcgcaatgg taatagtgat cttaatgtca
    76141 ccgttgtcga aacagacggt agttcgcaca actatattgt tccctccacc ctgtttaatc
    76201 agcatgtaga aagcttccag ggttatcgct tcgcgatagg gcgggtagac gatgactatg
    76261 acgaatcacc ttgggtaatt agtgcatcga gcggatggaa tctgacacgc tggagtgcaa
    76321 tgaacggcgg cgttatcgta gcagaaaatt atcaggcggc atcaatccgg tcgagtctgg
    76381 ttcccctgcc cgatttaaca gtgagcagcc aaattagtac atcgcaggat acgaaagact
    76441 cactgcaagg acagaaatat cgtcttgacg cgaactacaa tctcccattt tcacttgggc
    76501 taacaaccag cctcactcga tctgatcgcc attatcgcga actgtctgaa gcgattgatg
    76561 atgattatac cgatccgact aaaagcactt atgcgcttgg tttaaactgg tctaactcca
    76621 ttctgggtgg tttcaacatc agtggctata aaacatatag ttacgacggt gacaatgact
    76681 caagcaacct taatattaac tggaacaaag cgttcaaaca cgccacggtt tccgtcaact
    76741 ggcagcatca acttagtgct tcagaaaata atgaagacga tggtgatctg ttctacgtca
    76801 acatcagtat tccatttggc agatcaaaca ccgccacact gtatactcgc catgacgatc
    76861 ataaaaccca ctatggtact ggtgtcatgg gagtcgtctc agatgagatg tcctactatg
    76921 tgaatgctga acgagatcac gacgaacgtg aaacgagctt gaacggcagt atcagttcca
    76981 atctccatta cacccaagtc agccttgccg caggagcaag cggcagtgat agccgtactt
    77041 acaacggtac gatgtcaggt ggtattgccg tacatgatca gggagtgacc ttttcaccgt
    77101 ggactatcaa tgacactttc gccatcgcaa aaatggataa caatattgca ggtgtcagaa
    77161 ttacatctca ggcaggccca gtatggacag attttcgggg taatgccgtc attccatcaa
    77221 tccagccgtg gcgaacatca ggagttgaga tcgataccgc cagcttgcca aaaaatgtcg
    77281 atatcggtaa cggcacaaaa atgatcaaac aaggccgtgg tgcagtaggg aaagtcggat
    77341 tcagtgcgat aacacaacgc cgtgcattac tcaatatcac actttccgac ggcaaaaaac
    77401 tgcccagagg cgttgcgatt gaagatagtg aaggcaacta tctgacaaca tcagtggatg
    77461 acggtgttgt attcctcaat aacatcaaac cggacatggt gctagatata aaagatgagc
    77521 agcaatcatg ccgtattcac cttacattcc cagaagatgc accaaaagat gtgttctatg
    77581 agacagcaac aggagagtgc caatgaaacg gataataaca ggatgcctgc tgctgaactt
    77641 cgccatggcc gctcaggcag agtgtaatat ctccagtagc atacaaaata tcgactatgg
    77701 caaacgaagt gctgccatgc gccaagttga ccggggtaaa acaacacaat tagcagacag
    77761 aacaatcacg ctggtaatgc aatgcgatca ggacgctcat attcgtgttc agttgaatac
    77821 cgctaatatt tccaacaacg gatttggatt tggcccgaat ggttcattaa atctgatcgc
    77881 cagcgatgca ttttcaggta gtaacaatct cgacctggcg ttagccagtg gcaaaaatga
    77941 caatccgggc agcacaggaa cagcatcaat tagtacttcc cccaataatt ggctggtgtt
    78001 tatgcaaaat ggtcaagaag tggtaattga tagcggtaaa agcgttagcc tcacactgac
    78061 aatggctcca gcttttaagg atgaagggga actaaccgac atgacagata ttacaggcaa
    78121 tctgacggtc ctggtggagg caaaatgaac aatgtaaaat tactgattgc cggaagtgcc
    78181 ttttttgcca tgtcagcgca agccgctgat agagtatcaa ttgacgttaa ggtgactctg
    78241 gaagctgcag cttgtactcc aatactaagc aacggcggag ttgttaactt cggtagtcat
    78301 tcagtcaata gactttcaac gcagcactac acacagattg gaacacgtaa tattaatatg
    78361 actataactt gcgaatccgc taccggcatt gcgattaccg ctcgtgatac acgaatggat
    78421 agtatgacca ccggaaaaga tagtgggggt cagagtggtg taaaatatac cttaaatggc
    78481 ggaggttata ttagccagac aacgcgctta ttcggcttag ggaaaacaaa agataataaa
    78541 aatatcggta gttatgccgt gttaattgat agcaacaaca ttagtgccag caatggtagc
    78601 cagacgttag ctgtatcaat agcaggtgca gatgccgtca taacagggca aaaaagagca
    78661 tggcaaacgc tcaccgctta tccgcttgca gtcgatcaaa gctattatta cacctttgta
    78721 aaaccaggtg aaacaacccc aactcctgta acgaacgcca ttataccgct tcaggtgagc
    78781 gcatctatcg cgaatgattt aggcggttca gaaaaaatcg aacttgatgg gaaagctgtg
    78841 ataagcgtag tctacttgtg ataacaatgc cggacaggag tactatcagg cattcgtttt
    78901 atacgatcgg ttaatgtttc aggaaagctc ttcgcatatc ttggggaaag taaatgtaat
    78961 acgctgttct taattgttca gtgctcaccc cccagagtat agctcattcc attattcaac
    79021 ttagctccat ataaagggac agataatgga atcgctctca gaaggaacca cagcaggcta
    79081 ccagcaaatc cacgacggta ttattcatct ggtcgatagc gcccggacgg aaacggtacg
    79141 tagcgttaac gcgttaatga ccgcgacgta ccaggaaatt ggccgacgaa ttgtcgaatt
    79201 tgaacaaggt ggcgaggcca gggctgcgta tggtgcgcag ctaatcaagc gactatcaaa
    79261 ggatttatgt ctaaggtata agcgtgggtt ctctgcaaaa aacttacgcc aaatgaggct
    79321 tttttacctc ttttttcaac atgttgaaat tcgccagaca atgtctggcg aattaacacc
    79381 attgggaatt ccgcagacac cgtctgcaga attcccatcg gcaaagattt ggcagacgct
    79441 gtctgccaaa tctttcccct tgcccaggtc cacctacgtc cgtttacttt ccgttaaaaa
    79501 cgctgacgcc cgcagctttt atgaaaaaga gacgctccgc tgtggctggt ctgttcgcca
    79561 gctagagcgg caaattgcga cccagtttta tgagcggaca ctactgtcac atgacaaatc
    79621 agccatgctg caacaacacg ctcctgccga gacgcatatt cttccgcaac aggcgatacg
    79681 cgatcccttt gtgctcgaat ttctggaatt gaaagatgaa tattcagaat ccgattttga
    79741 ggaggcgctg atcaaccacc tgatggattt catgctggaa cttggggatg attttgcctt
    79801 tgttggtcgg cagcgaaggt tacgcattga tgacaactgg tttcgtgtcg atctgctgtt
    79861 tttccaccgc cgtttacgct gcctgctaat cgtcgatcta aaagtgggca aattcagcta
    79921 tagcgatgcc ggacagatga atatgtatct caactacgcc aaagagcact ggacgctacc
    79981 ggatgaaaat ccgcccatcg gtctggttct ctgtgcagag aaaggagccg gagaagcgca
    80041 ttatgctctg gcaggtttgc ctaacaccgt tctggcaagc gaatataaga tgcaactacc
    80101 tgatgagaaa cgactcgcag atgaactcgt tcgaacacag gcggtgctag aggaaggcta
    80161 tagacgccgt taatttcagc ggtttcgaat tctgcagaca gtgtctgcag aatctttaaa
    80221 acgaactaag ttttaagcca ttaaatcagc cttaaccttc accacaacct ttttaatctc
    80281 tccaggctcg ccgacaacgc atcctggttt atgcggttca cctggcataa acacggcgaa
    80341 cattcccggt tttaagatga tggcttgctc gttatcaatg gtgctgcaaa gctgataatc
    80401 atcctcatgg tggaactctt cacactgacg cgcagtgcct gccatgccaa acagaatccg
    80461 ttcctcaccg tttaataaca gctggatatc aatgtattgc tcgtgcaatt ccgctttttt
    80521 ctcgacgggc gattgagtgt taaacgtcat gacattcata aagatattgt cgccctgtaa
    80581 ttcgtaacga cccggcgctt tttcttgcgg tctggcagct aatgccagcg ttaacgcgtc
    80641 ctgtaacgca ggatgtaacc cagcagacgg taatgactgt acttcaccca tcatcataat
    80701 ttttctccct gggccaacag cgcagcccca agtaaacctg catcatggcg gtaatgcgcc
    80761 gccagtaaat caacatgaaa tgccgctggc tcctgcgcca gatacgtttc caccagcgcc
    80821 agataccctt ctgccagacc aacgctgcca ccgaccacca cgcactggca atcagttgtg
    80881 gctttaatat cagcgatcag ccttgcaagc gtacgtgcgg agcggtgaat cagctgctgc
    80941 gcctgctcgt caccctgccc ggcgcgcgtg aaaatagttt tcgcatccgc gccagccaac
    81001 tccccctgcg ctgccgctgc aatgccgcga ccagaagcaa tcgcttccac gcaacctgtg
    81061 cgtccacagc cgcagactgg gccgtgtgga tcggcaagcg tatgcccgat atgccccgcc
    81121 agaccgccag ggccggtaag cagtttgcag ccgctcacta caccgccgcc aacgccggtg
    81181 gaaacggtga taaagaccat atcggttata tcgccatcca gcgcctgaaa ctccgcccat
    81241 gctgcggcct gcgcgtcgtt aatggcaatg gtcggcaaat tggtaagttg ttccagcgtt
    81301 ttgactaacg gaaagtgtag caatccacca agattatgcg gattaagcgc cagcaagctg
    81361 ccgtcacgga ttatcccggt cgaagcgatg gcaacccgct gcgcatgagc ttgcaacgga
    81421 gagactaatg cggataaggc atcacgcaag gcttctggtg tctggctggc tggcgtagga
    81481 agttcacgac gatcgcggat ctgcccgtca gcgccaatca gcgcggcggc aagtttagta
    81541 ccgccgatat caatcgccag tgtggtcata gcaccgcctt tttcatcgct gtgttgtacc
    81601 actgacaaat gtgctcaaga cgcgtgattg cagaaccgac cgtcaccgcc cacgcgccgt
    81661 ggcgcatcgc atccgccgcc tgagcaggcg tgttgtaacg cccttcggca atcacccgac
    81721 atccggcgtc gctcaacgtt ttcaccagcg ccagatccgg ctcttctggc gtttcaggcg
    81781 tggtatagcc agaaagcgta gtgccaataa tttcggctcc cagcttttgg catgccaggc
    81841 cgtcttccgg cgttgagcag tcggtcatcg ccagtaaacc gtgatggtga atacgtgcca
    81901 gcagcgtttc aacaggcacc ggacgcgggc ggtcggtgcc gtcaatggcg ataatgtccg
    81961 cgcccgcctg cgccagcgca tcaacatctt caatataggc cgtgatgcgt accggagaat
    82021 cctccagatc gcgtttcaca attccaataa tcggcacgct caccaccgca cgcgtggctt
    82081 gcagatttgc cacaccttca atgcgaatgg caaccgcgcc cgcctgttct gccgctaatg
    82141 ccatggcggc gacgatttcg ggtttatcga gcgggctgtc cggaaccggc tggcaggaga
    82201 caatcaggcc accgttagca gcgatttttt gatccagttg tgcaagtaac gacatacatc
    82261 ttcccttagc gaaaggcccg gtacatagac cgggcaacag gattaacttt tggttttgac
    82321 taaatcgttt ttggcgctgc caaacggcac ggcaccgctg aatggtttac cgtcgatagc
    82381 gtcatgagta cgcaacgctt ccgggcgcaa ccaacgctga acgcgagaag gcatatccag
    82441 cccaatcagc aggatcacca cgaacgtcag actgaacgag agcgatgcca gcgcagtacc
    82501 cagatccaga cgttgagcga tcaacgcgcc gatgattggg gccagtgcac cgcccaatgc
    82561 gccaacgttg taggtaaagc ccaggcccgc tgcacgctgg tcggtatcga aataaccgcc
    82621 aatcagtttt ggtaagatcc cggcgatccc ttgtccaagc atttgctgga agaacagtaa
    82681 cagaccgagc acccagacgt ttgcgccgcc aatcgcaaat accggaataa tcagcagctg
    82741 cgaggccagc aggctacaaa cgtacgcttt gcgggttccc agccagtcac cgaggaagcc
    82801 acctacgcag catcccaccg ccgcgccaaa gccactaaag aacagcacat tggctacagt
    82861 atgcgggtta taagccagat cggttttcag atacgttggc agcagcgcct gaatcggcca
    82921 tgagtagagg aaagcaaaca acacgaccac catcagcatt acgcccgttg gccagcgttt
    82981 gcctgtactc tgcaccataa agctgataaa gattgcggcg cataacagcc caagaacagc
    83041 gacgatcgcg gcattttgca ggttaccggc gaagcagaac cacagcgcag tagccgccgc
    83101 cagtgtcatt acgatattgg caatgcgatg ttcaccacgg tagagaatat ccaccattgt
    83161 gcgtactggt gctttacctg cgtgtttctc tttccagtct tccgcttccg ggatgttttt
    83221 acgcagccag agagcaaaga tgattggcaa aatgccgata aagaacagcg cacgccagcc
    83281 ccagaccgga accaccaggc tatagacctg agcggcaacg acggccccca cagagaagcc
    83341 tgaaatcaaa aaaccactgg ctttgttacg cagatgtttt ggccagcttt caatgacata
    83401 ggtggcgctg gaaccgtatt cacccgccat ccccatgccg atgaccagac gagcgataaa
    83461 catggtgatg tagcctggcg caaagccgca ggccagcgtc ccggccgaga agagaacgat
    83521 gctggtgacc attgccagac gacgcccgta gcggtcaccc atagcgccga gcatcaggcc
    83581 gccgaaccag cgagagataa aggctgcaga gatcagactt gccgcctgca ccgtcgtcag
    83641 cccgaattca ccttgtactt cggtgagtac cagggcgatt aaaacgaaat caaaaccgtc
    83701 aagcagatat cccaaccagg cagcggaaaa tgcgcgccat tgtgcacggt tgagatggcg
    83761 ataccacggg atattctggg ttgtagtact cattgtgagt ctcccgcggt gggcgatgcc
    83821 cacacgcttt ggtatgaaaa ttgtagggta cagatgcgtt tatttcccct cacccggtag
    83881 gggcgagcga ggggaaacaa ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt
    83941 cagttctggc agatattttt catctaccgg tccaaacggt ttgcggcaca gcggcacaga
    84001 aacgacatcc atataatgga ggacagtttt caggccgcgg aatacgcccg ttttgatcag
    84061 taaatcaatg actttattgc attcagtttg cagtttctgc gcggtctgga tatcgccttc
    84121 tttcagcgcc ttaacgatcc cctgatagcg ccagcccatg atgttgtagg tactgccgat
    84181 accaccatca gcgcccgcca gcagaccaga ggcgaagatt tcgtcgtaac cgttatagag
    84241 cacaagatca ggatgttcac gacggatctg ctccatctga tagagatcgc cagaggtctg
    84301 tttcagcgca cctacgccag gcaatgtaac aagtgtgttg atctgatcca gggtcagttt
    84361 taccccactc agggctggaa tgttgtacac caccatcggc aaaccatccg ccgaatcaat
    84421 aattgcccga tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga acggcgtgac
    84481 ggcggagacg gcatcgaagc cataacgttt agccgatgcc gcaagttgtt ggctttcggc
    84541 ggtgctgacg caaccgacgt gggcgatgag tttaatctta cctttcgcct cttcggcgac
    84601 gatttccagt acctgttcac gctcggaaag gctttgtaca aaggcctcgc cggtcgaacc
    84661 acccacgtat aaaccgtcga tgccctgctg aatattgaac tgaaccaggc gacgcagact
    84721 cgctttatcc agtgcttgtt gttggtcaaa aggagtcagg agtgcagcca ttacgccacg
    84781 taaattcgtt gccataaata cctctgaagt gatgcttgtc tgataaacga tataccttta
    84841 tacctgttat accagatcaa ttaagcaaca ccccatacag aaagcttata atgcgatctg
    84901 cttcactaaa gtggcattat ttctttttgt tggtggtctg accgaaagcg tgccaggtag
    84961 cagagacgct gttgagatgc gattgcaacg cacgatcggc ttcgtcagga tcatgacggc
    85021 ggatcgcatc aacgatcgca atatgctgtt gataactaac gttgttatgt tcgtgcagtg
    85081 cctgatcggt aaccgttggg cgtgcggcaa taagccagtc gagcagggca acgtggatcg
    85141 ccatgaagat tgggttaccg gggatctccg ccagcacgcg gtggaaatca acgtctgaac
    85201 gaatgaatgc cgcgttgtta tccagcgact gactgttgat ttccagtgct tttgccagca
    85261 aatcgatttg ctcatcggtg gcatgttcag ccgcatagcg caccagactg gattcaaaga
    85321 acagacgtaa ttgttcgaaa tgggcaatcc caccgggatg agaaaggaaa tctttcgcca
    85381 tgccggaaag ctcaccgatg atagtgtccg cagaaggacg cgagacgcga gcgcgttcgc
    85441 cgttgtttat ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttca cgcaccgaag
    85501 gacgcccgac gttaaagaac gccatcagtt cgcgttcaga cggtaattgt tcaccttcgc
    85561 caaattcacg acggcggatc atctgttcca gctcttcttc caccatttcg gagagttttt
    85621 tacgcgccag cgggcggcta cgcaagttgc gaccaattgc aggtgaagaa tcttcggttt
    85681 gcgaatcaaa tgcgttcata aggcccattc tgtaaggtca gtgtgattaa catcatcagt
    85741 gacatcctat cacaggattg aaagtagggg aaaatggcag ggttttctct ttgtgcctca
    85801 tcattaccat aattaacgga ataattaact attgcgaaaa ttaatgtaac gcagataaaa
    85861 acatcccgtt tgaattattt ataagactat tcacgagcat tatgaatatt atgaatgtgt
    85921 tcttacaaaa taatcataag cgcatatttt ttaatgaaaa atcacctcac ctacaattaa
    85981 aaacacgaca tccgcaccat aaatagcctt gcaaaaaata taacatcgtt gttttcaatc
    86041 tgccgtttat gggattgacc gttttctttt gacacggagt tcaacaatgt tcggcataat
    86101 tatatctgtc atcgtattaa ttacgatggg ctatttgatc ctgaaaaact acaaacctca
    86161 ggtggtgctg gctgccgcag gtatcttcct gatgatgtgc ggtgtctggt tagggttcgg
    86221 tggtgtactc gatcccacca aaagcagcgg ctacttgatc gtcgatattt ataatgaaat
    86281 cctgcgcatg ctgtccaacc gcattgccgg attggggctg tcgattatgg cggtgggcgg
    86341 ttatgcccgc tacatggagc gcataggggc cagtcgcgcg atggtgagct tgttaagccg
    86401 cccgttaaaa ctcattcgct cgccgtatat tattctgtcg gcaacttacg tcatcggcca
    86461 aatcatggcg cagtttatta ccagcgcctc cggtctgggt atgttgctga tggtcacctt
    86521 atttccgacg ctggtgagtc tgggagtaag tcgtctctct gcggtggcag ttatcgcaac
    86581 cacgatgtcc attgagtggg ggattctgga aacgaactcc atttttgctg cccaggtagc
    86641 gggaatgaaa attgccacat acttcttcca ctaccagctt ccggtcgcct cttgcgtcat
    86701 tatctcggtg gcgatctccc actttttcgt gcaacgcgct tttgacaaaa aagataaaaa
    86761 tatcaatcac gaacaggcag agcaaaaagc tctcgataat gtcccgccgc tctattacgc
    86821 cattttacct gtgatgccgt taatcctgat gctcggctcg ctgttcctcg cccacgtcgg
    86881 gctgatgcag tcagaactgc atctggtggt ggtgatgtta ctgagtttga ctgtgacgat
    86941 gtttgttgag ttcttccgca agcataactt gcgcgaaaca atggacgatg tgcaggcgtt
    87001 ttttgacggc atgggtacgc agtttgccaa cgtggtaacg ctggtggtcg cgggtgaaat
    87061 atttgcgaaa ggcttaacga cgattggcac tgtcgatgcg gttatcaggg gggcggagca
    87121 ttctggtctg ggcggtattg gcgtgatgat tattatggcg ctggtcattg ccatttgtgc
    87181 cattgtgatg ggctctggca atgcgccgtt tatgtcattt gccagtctta ttccgaatat
    87241 cgcagccgga ctacatgtac cagcggttgt aatgattatg ccgatgcatt ttgccacgac
    87301 gctagcgcgc gcggtttcgc cgattactgc ggtggtggtc gttacgtcag gaattgcagg
    87361 cgtttcgcct tttgcggtgg tgaagcggac agcgatcccc atggcagtcg gtttcgtggt
    87421 gaatatgatt gccacaatca cgctatttta ttaagtcatt aaaaagacaa aacaggccgc
    87481 ctgggcctgt tttgtattac ttcacaacgc gtaatgccgg tcgaccaccg cgtggtggct
    87541 gcggaggttc atcgtcagga tgagtgtcat catcgtgatc tggcttgtcg ccatcaataa
    87601 ccgacataac ggtttcgttg tctgccgatg cctcttcatc attcatgatg ctggtatctt
    87661 catcgtaggc agcttcaggc tcaaacatcg tgcctgcgcc attttcacgg gcgtagatag
    87721 ccagcacggc agccagcggc acagaaacct gacgcggaat gccaccaaag cgcgcgttaa
    87781 agcgcacctc atcattcgcc agttccagat tgccgacagc acgcggcgca atgttgagta
    87841 cgatttgccc gtcacgcgca tattccatag gaacctgcac gccagggagc gtcacatcca
    87901 ccaccaggtg cggcgtgagc tggttatcca gcaaccactc atagaatgca cgcagcagat
    87961 agggacgacg tggtgttagc tgtgacaaat ccatacagat taactccggc ccagacgcat
    88021 ttcacgttct gcttcagtta aagaagcaag gaaagagtca cgctcaaaga cgcgggtcat
    88081 atagcctttc agctctttcg cacccgggcc gctgaactcg atgcccagtt gcggcagacg
    88141 ccacagcagc ggagcaagat agcaatcgac caggctgaac tcatcgctca ggaagtacgg
    88201 cttctgaccg aagaccggcg caatcgccag cagttcttcg cgcagttgct tacgtgcggc
    88261 atctgcttca gaagctgaac cgttgatgat ggtgttcatc agcgtgtacc agtctttttc
    88321 gatgcgatgc atgtacagac ggctttcacc gcgagctacc gggtaaacag gcatcagtgg
    88381 cggatgcggg aaacgctcat ccagatattc cataatgatg cgagattccc acagggtcag
    88441 ctcacgatcc accagggtcg gaacgctctg attcgggttg aggtcaatca gatcctgagg
    88501 cggattgtcc ttttccacgt gttcgatctc gaaacttaca cctttctcag ccagcacaat
    88561 gcggacctga tggctataga tgtcagtagg accggaaaac agcgtcatta ccgaacgttt
    88621 gttggcagcg acagccatga aaacctccag gtatagtcag aatttttact gctaccagcc
    88681 accaggtggc cagtcagaag ttgtgttacc caataaggaa cgactctctt tgttcgaaaa
    88741 tcaaacaaaa aatgagcaat acccgacatt tgggcagaaa attggatgat agtttaccag
    88801 attttgcgac cattgtggtg agtcgatgcc ggaaatgggg aaaaagagat gcgctttagt
    88861 ctgaaatagt tgacttagtc ccttattggc gatgtggttt ttgttttacc tgtctgtcag
    88921 gtggcagcaa aaagcaactt tccagttttt acgctgattc agattttagc tataaaaaaa
    88981 cccgccgaag cgggtttttt cgaaaattgt tttctgccgg agcagaagcc aattaacgtt
    89041 tggagaactg cggacgacga cgtgctttac gcagaccgac tttcttacgt tcaacctgac
    89101 gagcgtcacg agtaacgaag ccagctttac gcagttcaga acgcagggac tcgtcgtatt
    89161 ccatcagagc gcgggtgata ccgtgacgga tcgcaccagc ctgaccagag ataccaccac
    89221 ctttaacggt gatgtacagg tccagtttct caaccatgtc gaccagttcc agcggctgac
    89281 gaactaccat gcgggcagtt tcacgaccga agtactgttc cagagaacgt tggttgatta
    89341 cgattttacc gttgcccggt ttgatgaaaa cgcgagctgc ggaacttttg cggcgaccag
    89401 tgccgtagta ttgattttca gccattgcct ataatcccga ttagatgtca agaacttgcg
    89461 gttgctgtgc cgcgtggttg tgctcgttac ccgcgtaaac tttcagttta cggaacatag
    89521 cacgacccag cgggcctttt ggcaacatgc ctttaaccgc gatttcaatc acacgctcag
    89581 gacggcgagc aatcatctct tcaaaggtcg cttgtttgat accaccgatg tggccggtgt
    89641 ggtgatagta cactttgtca gtacgcttgt tgccggttac agcaactttg tcagcgttca
    89701 gaacgatgat gtaatcaccg gtatctacgt gcggagtgta ttccgctttg tgcttaccgc
    89761 gcaggcgacg agccagttca gtagccagac ggcccagagt tttaccggtc gcgtcaacaa
    89821 cataccagtc gcgttttacg gtttctggtt tagctgtaaa agttttcatt aaaagcttac
    89881 ccaataaata gttacacgtt ggtgaacacc caaacgtctt caattgttga ggttcacacg
    89941 acaaagtccg gcaaacctac cccttcgaat agcctatgcc agcacacaaa aagttttggg
    90001 aaaaaaactt tcttgtaacg tggggtcgca ggattataga gaagtcgggg tcaaagatcg
    90061 accccttttt gtgatttgtg acaggtttta acccgccaaa tgctcgcgct tcagatactc
    90121 ttcgctttgc atctcttgca gacgtgacag gcaacgctgg aactcaaact tcagccgatc
    90181 gccctgataa atttcataca gcggcacttc tgcactcacc actaatttga catggcgctc
    90241 gtaaaactca tccaccagcg caataaagcg ccgcgcttcg ctctccatca accgcgtcat
    90301 aactggtaca tcaaacaaca tgaccgtatg aaagagacgt gagagcgcaa tatagtcatg
    90361 ctgactgcgg gcgtcgacgc acagcgtagt aaaagagacc gccagcgtct ggttctcgac
    90421 gcccattgtt gctaatggcc gatggttgat ttctaacgtc ggtgaatttt ctcgtttccc
    90481 ccccgccagc gccaaccata gtttatccat ttgcgcccgg gtttcatcgt gaagtggcga
    90541 aagccacaga tgcgcctgag tgagtgtacg cagacgataa tcaacaccag cgtccacgtt
    90601 cattacatca caatgctgtt taatggcatc gattgcaggc agaaaacgcg cacgttgcag
    90661 gccatttcga taaagttcat ccggcggaat atttgacgtc gctaccaggg taataccgcg
    90721 agcgaacagg gctttcatca gaccgccaag tagcatggca tcggtaatat cagaaacaaa
    90781 aaattcgtca aaacagagca cgtcagtttc ggctttaaag cgatcggcaa taatttccag
    90841 cggatcggtc tgcccctgta aggcagttag ctcttcatgc acccgcagca taaaacggtg
    90901 aaagtgcagg cgctgtttcc gctctcccgg caggctttga tagaaaaggt ccatcagcca
    90961 ggttttcccg cgtcctacac cgccccacat atataagcca cgcactggcg tatgctttgt
    91021 gtcttcgcgt ttaccccaca gcttaccgac ccgcgccatt agcccactcg tcctgggggc
    91081 tggtggcgtg ctattgatga gttcctgata aataatttcc aggcggctga cggcctcttt
    91141 ttgaacgtcg tcgggttgat ggctgccttc attaagcgcc ttcaggtatt gcgatgttgg
    91201 ggtaacgctt tgcatgatct tattgttatt ccttgaataa tcggtgcgcc gttgttcacg
    91261 gttgacgaaa aaaaggccgt tctacactac gcgatatgca gtcgggattc cacttctgtg
    91321 gaattaacgg ttatagtggc ataatcagcc gcaggcatgg agcctgaagc caacacccta
    91381 cggaaacaaa agacaacggg agatgttcat gacctgggaa tatgcgctaa ttgggttagt
    91441 cgtcggcatc attattggtg ctgtggccat gcgttttggt aatcgtaaac tacgccagca
    91501 acaggcgttg cagtacgaac tggaaaagaa taaagctgaa ctggacgagt atcgcgaaga
    91561 gctggttagc cactttgccc gcagcgcgga attactggat accatggcgc acgactatcg
    91621 ccagctgtat cagcacatgg caaaaagctc cagcagcctg ctgccggaac tgtctgctga
    91681 agcaaacccg ttccgtaatc gtctggcaga gtctgaagcc agcaacgatc aggcaccggt
    91741 gcagatgcct cgcgactatt ctgaaggcgc atccggcctg ctgcgtactg gcgcgaagcg
    91801 cgactaattt atttttcggg cgcagccatt gcgccctcct cttctctccc tccccgacta
    91861 tcatttaatc tggtgtctca ttgttagccg tctgaaaatt caataacatc aaactgtttt
    91921 gaatctcttt tcttatcatt caggtacgag agcaggaata atgaaaaaac aaacccagct
    91981 gttgagtgca ttagcgttaa gtgtcgggtt aactctctcg gcgtcatttc aggccgtcgc
    92041 gtcgattcca ggccaggttg ccgatcaggc ccctctcccc agtctggctc caatgctgga
    92101 aaaagtgctt ccggcagtgg tgagcgtacg ggtggaagga acggccagtc agggacagaa
    92161 aatcccggaa gaattcaaaa agttttttgg tgatgattta ccggatcaac ctgcacaacc
    92221 cttcgaaggt ttaggctccg gtgtcatcat caacgccagt aaaggctatg tgctgaccaa
    92281 caaccatgtg attaatcagg cacagaaaat cagtattcag ctcaatgatg ggcgcgagtt
    92341 tgatgcaaaa ctgattggta gcgatgacca gagcgatatc gccctgttac aaattcaaaa
    92401 cccgagcaaa ttaacgcaaa tcgctattgc cgactccgat aaattgcgcg tcggtgattt
    92461 tgccgtagcg gtcggtaacc catttggcct tgggcaaacc gccacctctg gcattgtttc
    92521 cgcattaggc cgcagcgggt tgaatcttga aggtctggaa aactttatcc agacagatgc
    92581 ttccattaac cgcggtaact ccggcggtgc actattaaac cttaacggtg agttaattgg
    92641 catcaacact gcaatccttg cgcctggcgg cgggagcgtc gggattggat ttgccatccc
    92701 cagtaatatg gcgcgaacac tggcgcagca gcttatcgac tttggtgaaa tcaaacgcgg
    92761 tttgttaggc atcaaaggca ccgagatgag tgccgatatc gccaaagcct tcaaccttga
    92821 cgtgcagcgt ggcgcgtttg tcagcgaagt gttgccaggt tctggctcgg caaaagcggg
    92881 cgtcaaagcg ggcgatatta ttaccagcct caacggcaaa ccgctgaata gctttgctga
    92941 gttgcgctct cgtatcgcga ccaccgagcc gggcacgaaa gtgaagcttg gcctgctgcg
    93001 taacggcaaa ccactggaag tagaagtgac gctcgatacc agcacctctt cgtcggccag
    93061 cgctgaaatg atcacgccag cgctggaagg tgcaacgttg agcgatggtc agctaaaaga
    93121 tggcggcaaa ggtattaaaa tcgatgaagt tgtcaaagga agcccagctg ctcaggctgg
    93181 cttgcaaaaa gacgatgtga tcattggcgt caaccgcgat cgggtgaact cgattgctga
    93241 aatgcgtaaa gtgctggcgg caaaaccggc catcatcgcc ctgcaaattg tacgcggcaa
    93301 tgaaagcatc tatctgctga tgcgttaatg tcgtaaaccg ggcatcaggc ttacgtgtga
    93361 tgtccggtta actcgtggta tgctgctgcc gttccctttt ttaatgacgc ctccatcatg
    93421 tttgtgaagc tcttacgttc cgttgcgatt ggattaattg tcggcgctat tctgctggtt
    93481 gccatgcctt cgctgcgcag ccttaacccg ctttccactc cgcaatttga cagtaccgat
    93541 gagacgcctg ccagctataa tctggcggtt cgccgcgccg cgccagcggt ggttaacgtt
    93601 tacaaccgtg gtttgaacac caactctcac aaccagcttg agatccgcac cctgggatcc
    93661 ggtgtaatca tggatcaacg cggttatatc atcaccaata aacacgtcat caacgacgcc
    93721 gatcagatca tcgtcgcctt acaggatgga cgtgtatttg aagcattgct ggtgggatct
    93781 gactctctaa ccgatctggc ggtacttaaa attaatgcca ctggcggttt acctaccatt
    93841 ccaattaatg cacgtcgcgt accgcacatt ggcgacgtag tactggcgat cggtaacccg
    93901 tacaacctcg ggcagaccat tacccagggg attattagtg ccacgggtcg aatcggtctg
    93961 aacccgaccg ggcggcaaaa cttcctccaa accgatgctt ccattaacca cggtaactct
    94021 ggcggcgcgc tggtgaactc gctgggcgaa ctgatgggca ttaatacgct gtcgtttgat
    94081 aagagtaacg atggcgaaac gccggaaggt atcggctttg cgattccttt ccagttagca
    94141 accaaaatta tggataagct gatccgcgat ggtcgcgtga tccgcggcta cattggtatc
    94201 ggcggacgtg agatcgcacc actgcacgcg cagggcggtg gtatagatca actgcaaggg
    94261 atcgtggtta atgaagtgtc acctgacggc ccggcggcga atgcgggtat tcaggtcaac
    94321 gatctgatta tttcggtgga taacaaaccg gccatctctg ctctggagac gatggatcag
    94381 gtggcggaaa ttcgccctgg ttcggtgatc cctgtagtag tgatgcgtga tgataagcag
    94441 ttaacgctgc aggtcaccat tcaggaatat ccggcaacca attaagtcgt gcgctcaaaa
    94501 caaaaaaccg gagtctgtgc tccggttttt tattatccgc taatcaatta cttattaacg
    94561 aactcttcgc ccagggcgat atctttcttc agcgtatcca gcataccttc cagcgcgttc
    94621 tgttcaaatg cgctcagggt accgatagat ttacgctctt ccacgccgtt tttacccagc
    94681 agcagcggtt gagagaagaa acgggcgtac tgaccgtcgc cttcaacgta ggcacattcg
    94741 acaacgcctt gttcgccctg cagtgcacga accagagaca gaccaaaacg tgcagctgcc
    94801 tggcccatag acagggttgc agacccgcca ccggccttcg cttcaaccac ttcagtaccc
    94861 gcgttctgga tgcgtttggt cagatcagcc acttcctgct cggtaaaact aacgccagga
    94921 acctgtgaca gcagcggcag aatggtaaca ccagagtgac cgccaataac cggcacttca
    94981 acttcgcctg gctgtttgcc tttcagttcc gcaacaaagg tgttggaacg aatgatatcc
    95041 agcgtggtaa cgccgaacag tttgtttttg tcataaacac cggctttttt cagcacttca
    95101 gcagcaattg caactgtggt gttaaccggg ttagtgataa taccaatgca cgctttcggg
    95161 caggttttcg caacttgctg taccaggttt ttcacgatgc cggcgttaac gttaaacagg
    95221 tcggaacgat ccatacccgg tttacgcgct acgcctgcag agataagaac gacatctgcg
    95281 ccttccagcg ccggagtcgc atcttcacca gaaaaacctt tgattttcac agcagtaggg
    95341 atatggctca gatcgacagc cacaccggga gtcactggag cgatatcata cagagagagt
    95401 tctgaacctg aaggcagttg ggtttttaac agtagtgcaa gcgcctggcc aataccgcca
    95461 gcagcgccga ggactgcgac tttcatccta aactccttat tatattgata aactaagata
    95521 tgttgctccg ctgccgcgac cttaatccac aaaattgccg tttacaatga ccacatctca
    95581 agaatgtgta gtcacgcaag tttagcgttt atgcatttaa ttgccgtaat caggaaccta
    95641 acttacgtaa ttaacagtcg cttatgcatt agcgcaacat tccaacaggt ggtgacaata
    95701 taccctaccg ttcagccaaa acaacatcaa tttgataaca attaatttac ttttaagcag
    95761 aatttgcatg ccgtgacgca ggcatgtttc tcaataacga aatttgataa aatcccgctc
    95821 tttcataaca ttatttcagc cttcttcagg gctgactgtt tgcataaaaa ttcatctgta
    95881 tgcacaataa tgttgtatca accaccatat cgggtgactt atgcgaagct cggctaagca
    95941 agaagaacta gttaaagcat ttaaagcatt acttaaagaa gagaaattta gctcccaggg
    96001 cgaaatcgtc gccgcgttgc aggagcaagg ctttgacaat attaatcagt ctaaagtctc
    96061 gcggatgttg accaagtttg gtgctgtacg tacacgcaat gccaaaatgg aaatggttta
    96121 ctgcctgcca gctgaactgg gtgtaccaac cacctccagt ccattgaaga atctggtgct
    96181 ggatatcgac tacaacgatg cagttgtcgt gattcatacc agccctggcg cggcgcagtt
    96241 aattgctcgc ctgctggact cactgggcaa agcagaaggt attctgggca ccatcgctgg
    96301 cgatgacacc atctttacca cccctgctaa cggtttcaca gtcaaagacc tgtacgaagc
    96361 gattttagag ctgttcgacc aggagcttta atctctgccc cgtcgtttct gacggcgggg
    96421 aaaatgttgc ttatccctct caaccccctg ctttcccctg cgattaattt aacgaatagt
    96481 gcgttttact gcgacatgtc attcacacaa tgaatacata a
//
LOCUS       DBBGLQ010000006        93973 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.006, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000006 DBBGLQ010000000
VERSION     DBBGLQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 93973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.006"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            101..640
                     /gene="elfA"
                     /locus_tag="ACIYSB_000579"
     CDS             101..640
                     /gene="elfA"
                     /locus_tag="ACIYSB_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5427696.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQIDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            723..1424
                     /gene="elfD"
                     /locus_tag="ACIYSB_000580"
     CDS             723..1424
                     /gene="elfD"
                     /locus_tag="ACIYSB_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGC5427697.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKRKEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1449..4049
                     /gene="elfC"
                     /locus_tag="ACIYSB_000581"
     CDS             1449..4049
                     /gene="elfC"
                     /locus_tag="ACIYSB_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGC5427698.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNHLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGISWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQANSGIIDDDGSLYMAGLPAKGTISVRWGEAPDQICHINYELTEQQINS
                     AITRMDAICR"
     gene            4040..5109
                     /gene="elfG"
                     /locus_tag="ACIYSB_000582"
                     /pseudo
     CDS             4040..5109
                     /gene="elfG"
                     /locus_tag="ACIYSB_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5121..5663
                     /gene="ycbU"
                     /locus_tag="ACIYSB_000583"
     CDS             5121..5663
                     /gene="ycbU"
                     /locus_tag="ACIYSB_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5427699.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5671..6186
                     /gene="ycbV"
                     /locus_tag="ACIYSB_000584"
     CDS             5671..6186
                     /gene="ycbV"
                     /locus_tag="ACIYSB_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5427700.1"
                     /translation="MLKRIIWILFLLGLTWGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6152..6889
                     /gene="ycbF"
                     /locus_tag="ACIYSB_000585"
     CDS             6152..6889
                     /gene="ycbF"
                     /locus_tag="ACIYSB_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGC5427701.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            7000..8010
                     /gene="pyrD"
                     /locus_tag="ACIYSB_000586"
     CDS             7000..8010
                     /gene="pyrD"
                     /locus_tag="ACIYSB_000586"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGC5427702.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8184..8726
                     /gene="zapC"
                     /locus_tag="ACIYSB_000587"
     CDS             8184..8726
                     /gene="zapC"
                     /locus_tag="ACIYSB_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGC5427703.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8723..9832)
                     /gene="ycbX"
                     /locus_tag="ACIYSB_000588"
     CDS             complement(8723..9832)
                     /gene="ycbX"
                     /locus_tag="ACIYSB_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGC5427704.1"
                     /translation="MATLIRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10076..12184
                     /gene="rlmKL"
                     /locus_tag="ACIYSB_000589"
     CDS             10076..12184
                     /gene="rlmKL"
                     /locus_tag="ACIYSB_000589"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGC5427705.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYTNRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12196..14103
                     /gene="uup"
                     /locus_tag="ACIYSB_000590"
     CDS             12196..14103
                     /gene="uup"
                     /locus_tag="ACIYSB_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5427706.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLVDMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14233..15486
                     /gene="pqiA"
                     /locus_tag="ACIYSB_000591"
     CDS             14233..15486
                     /gene="pqiA"
                     /locus_tag="ACIYSB_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGC5427707.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15491..17131
                     /gene="pqiB"
                     /locus_tag="ACIYSB_000592"
     CDS             15491..17131
                     /gene="pqiB"
                     /locus_tag="ACIYSB_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGC5427708.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17128..17691
                     /gene="pqiC"
                     /locus_tag="ACIYSB_000593"
     CDS             17128..17691
                     /gene="pqiC"
                     /locus_tag="ACIYSB_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGC5427709.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17947..18114
                     /gene="rmf"
                     /locus_tag="ACIYSB_000594"
     CDS             17947..18114
                     /gene="rmf"
                     /locus_tag="ACIYSB_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGC5427710.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18184..18702)
                     /gene="fabA"
                     /locus_tag="ACIYSB_000595"
     CDS             complement(18184..18702)
                     /gene="fabA"
                     /locus_tag="ACIYSB_000595"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGC5427711.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18771..20531)
                     /gene="ycbZ"
                     /locus_tag="ACIYSB_000596"
     CDS             complement(18771..20531)
                     /gene="ycbZ"
                     /locus_tag="ACIYSB_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGC5427712.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20717..21169
                     /gene="matP"
                     /locus_tag="ACIYSB_000597"
     CDS             20717..21169
                     /gene="matP"
                     /locus_tag="ACIYSB_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGC5427713.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21245..22285)
                     /gene="ompA"
                     /locus_tag="ACIYSB_000598"
     CDS             complement(21245..22285)
                     /gene="ompA"
                     /locus_tag="ACIYSB_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGC5427714.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22642..23151)
                     /gene="sulA"
                     /locus_tag="ACIYSB_000599"
     CDS             complement(22642..23151)
                     /gene="sulA"
                     /locus_tag="ACIYSB_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGC5427715.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23370..23999
                     /gene="sxy"
                     /locus_tag="ACIYSB_000600"
     CDS             23370..23999
                     /gene="sxy"
                     /locus_tag="ACIYSB_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGC5427716.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTWERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23962..26124)
                     /gene="yccS"
                     /locus_tag="ACIYSB_000601"
     CDS             complement(23962..26124)
                     /gene="yccS"
                     /locus_tag="ACIYSB_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGC5427717.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDQSQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26134..26580)
                     /gene="yccF"
                     /locus_tag="ACIYSB_000602"
     CDS             complement(26134..26580)
                     /gene="yccF"
                     /locus_tag="ACIYSB_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGC5427718.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26703..28757
                     /gene="helD"
                     /locus_tag="ACIYSB_000603"
     CDS             26703..28757
                     /gene="helD"
                     /locus_tag="ACIYSB_000603"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGC5427719.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28789..29247)
                     /gene="mgsA"
                     /locus_tag="ACIYSB_000604"
     CDS             complement(28789..29247)
                     /gene="mgsA"
                     /locus_tag="ACIYSB_000604"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGC5427720.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29343..30005)
                     /gene="yccT"
                     /locus_tag="ACIYSB_000605"
     CDS             complement(29343..30005)
                     /gene="yccT"
                     /locus_tag="ACIYSB_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGC5427721.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30178..30591
                     /gene="yccU"
                     /locus_tag="ACIYSB_000606"
     CDS             30178..30591
                     /gene="yccU"
                     /locus_tag="ACIYSB_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGC5427722.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQKGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30636..30953)
                     /gene="hspQ"
                     /locus_tag="ACIYSB_000607"
     CDS             complement(30636..30953)
                     /gene="hspQ"
                     /locus_tag="ACIYSB_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGC5427723.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(31011..32200)
                     /gene="rlmI"
                     /locus_tag="ACIYSB_000608"
                     /pseudo
     CDS             complement(31011..32200)
                     /gene="rlmI"
                     /locus_tag="ACIYSB_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            32295..32573
                     /gene="yccX"
                     /locus_tag="ACIYSB_000609"
     CDS             32295..32573
                     /gene="yccX"
                     /locus_tag="ACIYSB_000609"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGC5427724.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32570..32899)
                     /gene="tusE"
                     /locus_tag="ACIYSB_000610"
     CDS             complement(32570..32899)
                     /gene="tusE"
                     /locus_tag="ACIYSB_000610"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGC5427725.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32990..33649)
                     /gene="yccA"
                     /locus_tag="ACIYSB_000611"
     CDS             complement(32990..33649)
                     /gene="yccA"
                     /locus_tag="ACIYSB_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGC5427726.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33856..33943)
                     /locus_tag="ACIYSB_000612"
     tRNA            complement(33856..33943)
                     /locus_tag="ACIYSB_000612"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33907..33909),aa:Ser,seq:tga)
     gene            34370..35488
                     /gene="hyaA"
                     /locus_tag="ACIYSB_000613"
     CDS             34370..35488
                     /gene="hyaA"
                     /locus_tag="ACIYSB_000613"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGC5427727.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            35485..37278
                     /gene="hyaB"
                     /locus_tag="ACIYSB_000614"
     CDS             35485..37278
                     /gene="hyaB"
                     /locus_tag="ACIYSB_000614"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGC5427728.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            37297..38004
                     /gene="hyaC"
                     /locus_tag="ACIYSB_000615"
     CDS             37297..38004
                     /gene="hyaC"
                     /locus_tag="ACIYSB_000615"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGC5427729.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            38001..38588
                     /gene="hyaD"
                     /locus_tag="ACIYSB_000616"
     CDS             38001..38588
                     /gene="hyaD"
                     /locus_tag="ACIYSB_000616"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGC5427730.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            38585..38983
                     /gene="hyaE"
                     /locus_tag="ACIYSB_000617"
     CDS             38585..38983
                     /gene="hyaE"
                     /locus_tag="ACIYSB_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGC5427731.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            38980..39837
                     /gene="hyaF"
                     /locus_tag="ACIYSB_000618"
     CDS             38980..39837
                     /gene="hyaF"
                     /locus_tag="ACIYSB_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGC5427732.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            39971..41515
                     /gene="appC"
                     /locus_tag="ACIYSB_000619"
     CDS             39971..41515
                     /gene="appC"
                     /locus_tag="ACIYSB_000619"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGC5427733.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRGLEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            41527..42663
                     /gene="appB"
                     /locus_tag="ACIYSB_000620"
     CDS             41527..42663
                     /gene="appB"
                     /locus_tag="ACIYSB_000620"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGC5427734.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            42676..42768
                     /gene="cbdX"
                     /locus_tag="ACIYSB_000621"
     CDS             42676..42768
                     /gene="cbdX"
                     /locus_tag="ACIYSB_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGC5427735.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            42848..44146
                     /gene="appA"
                     /locus_tag="ACIYSB_000622"
     CDS             42848..44146
                     /gene="appA"
                     /locus_tag="ACIYSB_000622"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGC5427736.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(44261..46441)
                     /gene="etk"
                     /locus_tag="ACIYSB_000623"
     CDS             complement(44261..46441)
                     /gene="etk"
                     /locus_tag="ACIYSB_000623"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGC5427737.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(46461..46907)
                     /gene="etp"
                     /locus_tag="ACIYSB_000624"
                     /gene_synonym="yccY"
     CDS             complement(46461..46907)
                     /gene="etp"
                     /locus_tag="ACIYSB_000624"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGC5427738.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(46895..48034)
                     /gene="gfcE"
                     /locus_tag="ACIYSB_000625"
     CDS             complement(46895..48034)
                     /gene="gfcE"
                     /locus_tag="ACIYSB_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGC5427739.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(48080..50176)
                     /gene="gfcD"
                     /locus_tag="ACIYSB_000626"
     CDS             complement(48080..50176)
                     /gene="gfcD"
                     /locus_tag="ACIYSB_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGC5427740.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDACWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(50176..50922)
                     /gene="gfcC"
                     /locus_tag="ACIYSB_000627"
     CDS             complement(50176..50922)
                     /gene="gfcC"
                     /locus_tag="ACIYSB_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGC5427741.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(50919..51563)
                     /gene="gfcB"
                     /locus_tag="ACIYSB_000628"
     CDS             complement(50919..51563)
                     /gene="gfcB"
                     /locus_tag="ACIYSB_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGC5427742.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(51670..51975)
                     /gene="gfcA"
                     /locus_tag="ACIYSB_000629"
     CDS             complement(51670..51975)
                     /gene="gfcA"
                     /locus_tag="ACIYSB_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HGC5427743.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(52417..52629)
                     /gene="cspH"
                     /locus_tag="ACIYSB_000630"
     CDS             complement(52417..52629)
                     /gene="cspH"
                     /locus_tag="ACIYSB_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGC5427744.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            52915..53127
                     /gene="cspG"
                     /locus_tag="ACIYSB_000631"
     CDS             52915..53127
                     /gene="cspG"
                     /locus_tag="ACIYSB_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGC5427745.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            53138..53326
                     /gene="ymcF"
                     /locus_tag="ACIYSB_000632"
     CDS             53138..53326
                     /gene="ymcF"
                     /locus_tag="ACIYSB_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGC5427746.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQICTDHAPLDFTK"
     gene            53301..53531
                     /gene="ymcE"
                     /locus_tag="ACIYSB_000633"
     CDS             53301..53531
                     /gene="ymcE"
                     /locus_tag="ACIYSB_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGC5427747.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            53521..53694
                     /gene="gnsA"
                     /locus_tag="ACIYSB_000634"
     CDS             53521..53694
                     /gene="gnsA"
                     /locus_tag="ACIYSB_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGC5427748.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(53743..54816)
                     /gene="yccM"
                     /locus_tag="ACIYSB_000635"
     CDS             complement(53743..54816)
                     /gene="yccM"
                     /locus_tag="ACIYSB_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGC5427749.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIGCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(54888..57632)
                     /gene="torS"
                     /locus_tag="ACIYSB_000636"
     CDS             complement(54888..57632)
                     /gene="torS"
                     /locus_tag="ACIYSB_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGC5427750.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            57715..58743
                     /gene="torT"
                     /locus_tag="ACIYSB_000637"
     CDS             57715..58743
                     /gene="torT"
                     /locus_tag="ACIYSB_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGC5427751.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(58716..59408)
                     /gene="torR"
                     /locus_tag="ACIYSB_000638"
     CDS             complement(58716..59408)
                     /gene="torR"
                     /locus_tag="ACIYSB_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGC5427752.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QSVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            59538..60710
                     /gene="torC"
                     /locus_tag="ACIYSB_000639"
     CDS             59538..60710
                     /gene="torC"
                     /locus_tag="ACIYSB_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGC5427753.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            60710..63256
                     /gene="torA"
                     /locus_tag="ACIYSB_000640"
     CDS             60710..63256
                     /gene="torA"
                     /locus_tag="ACIYSB_000640"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGC5427754.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGLRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            63253..63852
                     /gene="torD"
                     /locus_tag="ACIYSB_000641"
     CDS             63253..63852
                     /gene="torD"
                     /locus_tag="ACIYSB_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGC5427755.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(64004..64309)
                     /gene="cbpM"
                     /locus_tag="ACIYSB_000642"
     CDS             complement(64004..64309)
                     /gene="cbpM"
                     /locus_tag="ACIYSB_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGC5427756.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(64309..65229)
                     /gene="cbpA"
                     /locus_tag="ACIYSB_000643"
     CDS             complement(64309..65229)
                     /gene="cbpA"
                     /locus_tag="ACIYSB_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGC5427757.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            65490..66746
                     /gene="yccE"
                     /locus_tag="ACIYSB_000644"
     CDS             65490..66746
                     /gene="yccE"
                     /locus_tag="ACIYSB_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HGC5427758.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVRDIDIVRVSLPGRASESITDFKGYYWYNFMEYIENINACDDVFSEYCFD
                     DENISVQPERINTPGISDLDSDIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAIL
                     VLKQHDYSWALVSYKMQKEVYNEFIKMVDKKTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNQIQGQSSLMSWSFLFFKKQFIDSCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLMNTGNHYDVYLPP
                     ELFAQAYKLNNKEMNAQLDYLNRYAI"
     gene            67039..68280
                     /gene="agp"
                     /locus_tag="ACIYSB_000645"
     CDS             67039..68280
                     /gene="agp"
                     /locus_tag="ACIYSB_000645"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGC5427759.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(68318..68545)
                     /gene="yccJ"
                     /locus_tag="ACIYSB_000646"
     CDS             complement(68318..68545)
                     /gene="yccJ"
                     /locus_tag="ACIYSB_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGC5427760.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(68566..69162)
                     /gene="wrbA"
                     /locus_tag="ACIYSB_000647"
     CDS             complement(68566..69162)
                     /gene="wrbA"
                     /locus_tag="ACIYSB_000647"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGC5427761.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            69535..69708
                     /gene="ymdF"
                     /locus_tag="ACIYSB_000648"
     CDS             69535..69708
                     /gene="ymdF"
                     /locus_tag="ACIYSB_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGC5427762.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(69807..69905)
                     /locus_tag="ACIYSB_000649"
                     /pseudo
     CDS             complement(69807..69905)
                     /locus_tag="ACIYSB_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(69965..71293)
                     /gene="rutG"
                     /locus_tag="ACIYSB_000650"
     CDS             complement(69965..71293)
                     /gene="rutG"
                     /locus_tag="ACIYSB_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGC5427763.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLITVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(71314..71808)
                     /gene="rutF"
                     /locus_tag="ACIYSB_000651"
     CDS             complement(71314..71808)
                     /gene="rutF"
                     /locus_tag="ACIYSB_000651"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGC5427764.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(71819..72409)
                     /gene="rutE"
                     /locus_tag="ACIYSB_000652"
     CDS             complement(71819..72409)
                     /gene="rutE"
                     /locus_tag="ACIYSB_000652"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGC5427765.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVRDETLREIYALMKWGP
                     TSANCSPARIVFTRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            complement(72419..73219)
                     /gene="rutD"
                     /locus_tag="ACIYSB_000653"
     CDS             complement(72419..73219)
                     /gene="rutD"
                     /locus_tag="ACIYSB_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGC5427766.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(73227..73613)
                     /gene="rutC"
                     /locus_tag="ACIYSB_000654"
     CDS             complement(73227..73613)
                     /gene="rutC"
                     /locus_tag="ACIYSB_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HGC5427767.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(73625..74317)
                     /gene="rutB"
                     /locus_tag="ACIYSB_000655"
     CDS             complement(73625..74317)
                     /gene="rutB"
                     /locus_tag="ACIYSB_000655"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGC5427768.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPKFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFAHIA"
     gene            complement(74317..75408)
                     /gene="rutA"
                     /locus_tag="ACIYSB_000656"
     CDS             complement(74317..75408)
                     /gene="rutA"
                     /locus_tag="ACIYSB_000656"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGC5427769.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            75696..76334
                     /gene="rutR"
                     /locus_tag="ACIYSB_000657"
     CDS             75696..76334
                     /gene="rutR"
                     /locus_tag="ACIYSB_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGC5427770.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(76374..80336)
                     /gene="putA"
                     /locus_tag="ACIYSB_000658"
     CDS             complement(76374..80336)
                     /gene="putA"
                     /locus_tag="ACIYSB_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGC5427771.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEESDRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAKYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGTIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            80391..80600
                     /locus_tag="ACIYSB_000659"
     CDS             80391..80600
                     /locus_tag="ACIYSB_000659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427772.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            80759..82267
                     /gene="putP"
                     /locus_tag="ACIYSB_000660"
     CDS             80759..82267
                     /gene="putP"
                     /locus_tag="ACIYSB_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGC5427773.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNDHPALAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            82810..83640
                     /gene="efeU"
                     /locus_tag="ACIYSB_000661"
     CDS             82810..83640
                     /gene="efeU"
                     /locus_tag="ACIYSB_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGC5427774.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            83698..84825
                     /gene="efeO"
                     /locus_tag="ACIYSB_000662"
     CDS             83698..84825
                     /gene="efeO"
                     /locus_tag="ACIYSB_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGC5427775.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDDFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            84831..86102
                     /gene="efeB"
                     /locus_tag="ACIYSB_000663"
     CDS             84831..86102
                     /gene="efeB"
                     /locus_tag="ACIYSB_000663"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGC5427776.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWAIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYFGSALLRV"
     gene            86723..87511
                     /gene="phoH"
                     /locus_tag="ACIYSB_000664"
     CDS             86723..87511
                     /gene="phoH"
                     /locus_tag="ACIYSB_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGC5427777.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(87561..87974)
                     /gene="pgaD"
                     /locus_tag="ACIYSB_000665"
     CDS             complement(87561..87974)
                     /gene="pgaD"
                     /locus_tag="ACIYSB_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HGC5427778.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(87976..89301)
                     /gene="pgaC"
                     /locus_tag="ACIYSB_000666"
     CDS             complement(87976..89301)
                     /gene="pgaC"
                     /locus_tag="ACIYSB_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGC5427779.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(89294..91312)
                     /gene="pgaB"
                     /locus_tag="ACIYSB_000667"
     CDS             complement(89294..91312)
                     /gene="pgaB"
                     /locus_tag="ACIYSB_000667"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HGC5427780.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            complement(91321..93744)
                     /gene="pgaA"
                     /locus_tag="ACIYSB_000668"
     CDS             complement(91321..93744)
                     /gene="pgaA"
                     /locus_tag="ACIYSB_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HGC5427781.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDIDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGISWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            complement(93751..>93973)
                     /locus_tag="ACIYSB_000669"
     CDS             complement(93751..>93973)
                     /locus_tag="ACIYSB_000669"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427782.1"
                     /translation="WDLCRIPEDPHHWNGFSGEKRILYGHSAHHFFFSSSTIHVSLPR
                     LITDYEVQKYLSFQLPVIRYRERFWQYME"
BASE COUNT        23208 a        23283 c        24535 g        22947 t
ORIGIN      
        1 ttagtatgct tgatttaaat ggcggacaat tattgttatt attaagtcta atgtcattta
       61 aaggcattat catatttacg aagagatcag gatgataacg atgaaaaaaa gtgtattgac
      121 ggcgtttata actgtggtat gtgcaacgtc cagcgttatg gctgctgatg ataatgctat
      181 cacggatggc tcagtaacat ttaatggtaa agttattgct ccagcttgta ccctggtagc
      241 tgcgacgaaa gattccgtgg tgactttgcc agatgttagt gccacgaagt tgcaaaccaa
      301 tggtcaggtt tctggcgtgc aaattgatgt gccaattgaa ttaaaagatt gtgatactac
      361 cgtaacaaaa aatgcaacgt tcacctttaa tggcactgcg gatactactc agattacagc
      421 gtttgctaac caggcctcat ctgatgctgc tacaaacgtg gccctgcaaa tgtatatgaa
      481 tgatggtaca acggccatca cgccagacac agaaaccggg aacattttgt tgcaggatgg
      541 agatcagacg ttgactttta aagttgatta tatcgctacg gggaaagcga cttcaggtaa
      601 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      661 attaattagt acccctccag tgttctggag gggatattca tattttttaa gagtgactat
      721 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      781 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      841 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      901 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gactttatta
      961 ttacaccacc gctatttgtg ctgaatccgg caaatgagaa tctgttacgc attatgtaca
     1021 ttggagcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1081 cttcaacaac gaagcggaaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1141 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1201 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1261 tgacagacct gaaaataggt ggaaaagtag ttaaaaatca aatgatttcc ccctttgata
     1321 aataccaatt ttctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1381 atgactacgg ggcggaaacg ccgcaactca actgtaaatc gtaagccgtc ttcagttaag
     1441 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1501 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1561 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1621 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1681 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1741 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1801 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1861 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1921 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1981 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     2041 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2101 caacatggag tcgtagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2161 aacgggcggt tattcctctg aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2221 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2281 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2341 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2401 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2461 cggacggcag cgtcaatagc tacagcgtac cgttctccag tgtgccatta ctccagcgtc
     2521 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2581 aggagagcaa atttgcccag gccacgttgc agtggggcgg accgtgggga acgacctggt
     2641 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2701 tcggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctagcagatc
     2761 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccacttgg
     2821 gcactaactt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2881 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2941 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     3001 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3061 ggcatacgga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3121 tttcgctggg gatttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3181 tgtttgctct aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3241 ataccaggaa aaaaaattac gcctggatga cctctaacac cagtatcgat aacgaagggc
     3301 acactacaca aaacctgggt ttaacggaga cattactcga tgacggtaac ctgagctaca
     3361 gcgtgcaaca gggatataac agcgagggga aaacggctaa tggtagcgcc agcatggatt
     3421 acaaaggggc gtttgcggat gcccgagtgg gctacaacta cagcgataac ggcagtcaac
     3481 aacaactgaa ctacgctctt tcaggcagtt tagttgccca ttcacagggc attaccctgg
     3541 ggcaatcgct gggggaaact aacgttctga ttgcagcacc aggcgcagag aatactcgtg
     3601 tggcgaacag caccgggctg aaaactgact ggcgcggata taccgttgtt ccttatgcca
     3661 cttcttatcg ggaaaatcga atcgcacttg atgcggcgtc gttaaaacgt aacgtggatc
     3721 ttgaaaatgc agtagtcaac gtggttccca ccaaaggggc gttggttctg gcggagttca
     3781 atgcccatgc gggtgcaagg gtattaatga aaacatcaaa gcagggtata ccgctgcgtt
     3841 ttggcgcgat agcgacgctg gacggcgtac aggctaatag cggcataatt gatgatgatg
     3901 gctcgctcta tatggcgggt ttaccggcga agggaacaat aagcgtgcgc tggggcgaag
     3961 ctcccgatca aatttgtcat atcaattacg agcttaccga acaacaaatt aactctgcga
     4021 ttacgcgaat ggatgccata tgcagataat ctttggagaa aaatgcgtgt cattactacg
     4081 actatttttt gccgccgtct taatgctatg gtgcgctcaa accgctgctt atagcgggca
     4141 gtgtcatact actcagggga atccgtatat tggcgtcaat tttggcgtta aaaccctgga
     4201 ggaagaagca aatacggcag gggtagttaa agacaaattt tatcagtgga acgaatcgaa
     4261 tgattattat gtttcctgtg attgcgataa agacaatgtc agaagtggcc gatgggcatt
     4321 cgccgcggat tcaccgttag tctatttagg cgacaactgg tacaaaatta atgactatct
     4381 tgccgccaaa gttttattgc aggttaaagg cagttctcct actgcggttc ctttcgaaaa
     4441 cgtgggcaca ggggggatac ccgatggcat atttgcgacc ctggcggtca acgtttaggt
     4501 gggcaggggg caagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4561 ggctcggtcg tcattcctcc tatggcgctg gcgcgattat atgaatgcta caacataccc
     4621 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4681 atcaattcac ttggctcatg ttccgtcaat gccggagaga caattgaagt tgatttaggt
     4741 gatgtcttcg ctgccaattt ccgtgttgta gggcataaac ctcttggggc cagaacggca
     4801 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg gattagttaa tgtcaacctg
     4861 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4921 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4981 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     5041 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5101 tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt atcttgttct
     5161 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5221 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5281 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5341 atttgaatat cgatatcacc ggttgtgccg ctggtattac tggcgcggac gtccttttta
     5401 gcggcgaggc tgacaccctt gcgccgacac tgctcaaact aactgacaca ggcggaagcg
     5461 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5521 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5581 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5641 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5701 ttattgggat taacgtgggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5761 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5821 ttaggcgatg tctcgctgac acgtttttct catgggaatt atggcccgga gaaatctttc
     5881 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5941 acccccgacg gagtgcagag cgaaatgttg tccattgaaa gtggtacgga tgcagccagc
     6001 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6061 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6121 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6181 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6241 gggtgaattg caggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6301 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6361 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6421 ggccgctccg ccacttattc tcctgaagcc cggtacgaca ggcacgttgc gcttgctgag
     6481 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6541 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6601 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6661 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttatt acattaacct
     6721 gattcaggtg agtgtaaatg gtaaagcgtt aagtaatgtg ggagttgtgc cgcctaaaag
     6781 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6841 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6901 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6961 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     7021 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7081 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7141 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7201 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7261 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7321 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7381 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7441 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7501 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7561 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7621 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7681 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7741 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7801 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7861 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7921 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7981 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     8041 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8101 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8161 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8221 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8281 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8341 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8401 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8461 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8521 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8581 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8641 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8701 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8761 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     8821 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8881 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8941 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     9001 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9061 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9121 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9181 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9241 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9301 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9361 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9421 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9481 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9541 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9601 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9661 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9721 tcataaagat gcgatcgaag gccagaccac tgacatctgc cagagcatgt gtaagaccaa
     9781 tgccgcgcat cgatttaaca ggatgaataa aaagccggat taatgtcgcc acggtgcggt
     9841 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9901 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9961 atgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
    10021 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10081 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10141 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10201 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10261 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10321 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10381 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10441 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10501 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10561 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10621 gatgcgatcc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10681 gttgctgatt gaagcagcga tgctggcgac cgatcgcgca ccaggcttgc accgtgggcg
    10741 ttggggcttt agcggctggg cgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10801 agcgcaaact cgcgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10861 cagcgacgca cgggtgattc aacgtgcacg cactaacgcc cgtcttgcgg ggattggtga
    10921 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10981 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    11041 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11101 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11161 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11221 cactccagac agcaaaccgg cgatggtagc ggaagactac accaaccgcc tgcgtaagaa
    11281 cctcaaaaaa ttcgagaagt gggctcgcca ggaagggatt gaatgttacc gcctgtatga
    11341 cgccgatctg ccagaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11401 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11461 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11521 tgaacgccag aagggcaaaa accaatacca gaaactgggc gagaagggcg agtttcttga
    11581 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11641 gttcctcgat caccgcatcg cccgtcgtat gctcggtcag atgagcaaag gcaaagattt
    11701 cctcaacctg ttctcttata ccggcagcgc caccgtgcat gcgggattag gcggtgcacg
    11761 cagcaccacc accgtggata tgtcgcgtac ttatctggaa tgggcagaac gcaacctgcg
    11821 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11881 gcgtgaggca aatgaacagt tcgatctgat ctttatcgat ccgccaacct tctctaactc
    11941 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat ctggcgctga tgaaagattt
    12001 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12061 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12121 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12181 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12241 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12301 ggccgcaacg gcgcaggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12361 gatgacggtc gcattattta cgagcaagat ttgattgtag cgcgtctgca acaggatccg
    12421 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12481 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12541 aatctcaacg aactggcgaa ggttcaggaa cagctggatc accacaacct gtggcagctg
    12601 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaacgt tgcgctgtcg
    12661 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12721 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12781 gaagggtttt tgaaaacttt caacgggacg attattttca tctcccacga ccgttcgttt
    12841 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12901 gggaattacg accagtacct gctggaaaaa gaagaagccc tgcgcgtgga agaattacaa
    12961 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    13021 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcgatgcg tcgcgaacgt
    13081 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13141 ggtaaaatcg ttttcgaaat ggaagacgtt tgctaccagg ttaacggtaa gcaactggtg
    13201 aaagattttt ctgcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13261 tgcggcaaaa ccacgctgct aaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    13321 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13381 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13441 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13501 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13561 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13621 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13681 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13741 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13801 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13861 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13921 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaagtggc ggatgcttcc
    13981 ttcttcagtc agccgcatga gcagacgcaa aaagtgcttg ttgatatggc tgctgcagag
    14041 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14101 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14161 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    14221 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14281 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14341 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtcccaccgc ctatgcgttg
    14401 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14461 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14521 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14581 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14641 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc gggtgtgctg
    14701 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14761 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14821 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14881 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14941 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    15001 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15061 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15121 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15181 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15241 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15301 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15361 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15421 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15481 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15541 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15601 ttacagccat cagggaccgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15661 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15721 ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg aattccggta tggaaaaatt
    15781 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15841 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15901 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15961 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    16021 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacac aaaaacgcaa
    16081 tatcagctat caactgttca tcaatgcacc ttatgaccga ctggtgacca acaatgttcg
    16141 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcagggatgc gtgtggagat
    16201 gggctcattg acaacgctgc tgagtggcgg tgtcagcttt gatgtgccgg aaggtctgga
    16261 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16321 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16381 cggtctgcaa ccgggagctc cggtagagtt ccggggtatt cgcctgggta ccgtaagcaa
    16441 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16501 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16561 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16621 ggtcactggt gcactgtatg ttgatctcga tttctatcca aatacgcctg caataaccgg
    16681 tattcgtgaa tttaatggtt atcagattat cccgaccgtt agcggcggcc tggcgcaaat
    16741 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16801 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16861 gctggatagc atgaacaaga tcctcgctag ccagtcgatg cagcagttgc cgacggatat
    16921 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16981 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    17041 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17101 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17161 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17221 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17281 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17341 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacagttgcg caacaccctg
    17401 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17461 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17521 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17581 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtgct ggccggtgtc
    17641 tggagtcagg aagccgcttc tattgcacaa gagataaagc gtctacctta attataaaga
    17701 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtc tctaacgtgc
    17761 ggaaaaattt gttcctcttc acattttttg tacaaccgac atgcccgtgt agctcacaaa
    17821 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17881 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17941 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    18001 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18061 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18121 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18181 taatcagaag gcagacgtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18241 cagacgacca tcaaccagca cttcgccatc cgccaggccc ataatcagac gacggttaac
    18301 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18361 gaatttcact tcgccaacgc ccagcgcgcg gcctttacct tcgccgccca gccagccgag
    18421 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18481 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18541 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18601 catgttcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18661 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18721 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18781 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18841 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18901 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18961 ttgccttctt ctaccgcttt caccagttca ctgtgaagac ttaaatggcg aacgttagct
    19021 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19081 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19141 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19201 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19261 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19321 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19381 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19441 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19501 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19561 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19621 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19681 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtatcggcca ggcatccgct
    19741 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19801 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19861 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19921 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19981 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    20041 tgcgccagca gtgtacgcaa agagataatg agaataccgc catttgcctg atgcaccaga
    20101 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20161 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20221 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20281 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20341 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20401 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20461 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20521 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20581 gcactacagc acacgtagaa agcaccgaca atactcctgg catgggcgtt aaagctcaca
    20641 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20701 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20761 gaagtatctg gttaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20821 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20881 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20941 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    21001 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21061 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21121 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21181 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21241 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21301 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21361 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21421 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21481 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21541 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21601 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21661 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21721 aggaaacacc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21781 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21841 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21901 atttagtgtc tgcacgccat accatgccac ccagacgagt gtagatgtcc aggtcgtcag
    21961 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    22021 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22081 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22141 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22201 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22261 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22321 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caatattaac
    22381 tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct acaacgtagt
    22441 tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg tataaggtat
    22501 gtttaatctt ttttgtcagc gacaatttac agaagagaat cgcggaaacc gcttcagaca
    22561 agcctccgca aggaaaatta gtcacgactg aaagcattgg ctgggcgaca aaaaaagttc
    22621 caggattaat cctaaattta cttaatgata caaattagag tgaattttta gcccggaaag
    22681 ttgtctcgtg gcgtgagagg atgcgcttac cggacgcata ataaacccca tagcgttacc
    22741 ttcatttgcc gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat ctgccaacca
    22801 accgatcacc acactgtaat tgcccgtgcg taaagcgcga accattgact ccacagtgtg
    22861 gcaaggggag agctggctaa tctgcattac tttcgttaag ggtagcccag atgcctgaac
    22921 ccattcccga ctcagttttt gttgcggtgt taaccagagt tgccagcgcg attgctgacc
    22981 gagttgctgt aacaatggca acagtagaag ttgcgtcatc atgggctgat cttcgcgata
    23041 gacaacttca ctgataagcc cggctgtagt gttttccgta gagacacgcg caattttact
    23101 tgctgcggat gagaacgacg aagaacgatg tgcatagcct gaagtgtaca taatcaatcc
    23161 agcccctgtg agttactgta tggatgtaca gtacatccag tgacaacaaa gatcaaccct
    23221 attttcggaa agagcctcgc aaattttgtc gttggtgacg ggaaaacata aattaatctt
    23281 gccccttaag aataagttgc ctattttcgt agttaacgga tccgttaatg tgaatcattc
    23341 ttttatgtta tgattttaaa aggaatttta tgaaaagcct ctcctataag cggatctata
    23401 aatcacaaga atacctggca acgttgggca caattgaata ccgatcattg tttggcagtt
    23461 acagcctgac cgttgacgac acggtgtttg cgatggtttc tgatggtgag ttgtatcttc
    23521 gggcttgtga gcaaagtgca cagtactgtg taaaacatcc gcctgtctgg ctgacatata
    23581 aaaagtgtgg ccgatccgtt accctcaatt actatcgggt tgatgaaagt ctatggcgaa
    23641 atcaactgaa gctggtgcgt ctgtcgaaat attctctcga tgcagcgctg aaagagaaaa
    23701 gcacgcgcaa tacctgggaa agactgaaag atttgcccaa tatgtctttt catctggaag
    23761 cgattctcgg ggaggtgggg attaaggatg tacgggcgtt acgtatactt ggggcaaaaa
    23821 tgtgttggtt gcgactgcgg cagcaaaaca gtctggtgac agaaaagatt ctgtttatgc
    23881 ttgaaggtgc cattatcggc attcatgaag ctgcgctccc ggtggcacgc cgccaggagc
    23941 ttgcagaatg ggctgactct cttacgccga aacaggagtt tcctgcggaa cttgagtaat
    24001 ctggcgttgc agacgaccaa tctcaggcag taatgcaatc aataatccaa cttgttgtac
    24061 gaccagaggt tctttgctgt ctgcccgtgg ttcaagttgc tgcatccgct gtttcaggct
    24121 agctaatgcc tcattgacgc gttcttcatc agcaggttga tgatgtaacg cgtcatcaac
    24181 atagcaaact gcgtcatcaa gaaacgccag aatttcagga ttagttaact gctcccggtg
    24241 agcaccgagg gctgagatat agctggtaaa cgtatggtta aggcacagca accgaaacgc
    24301 ggcttcgcga atttgcgggg taacgttcgg ctcgctggac atatttgata ccaccgacgc
    24361 cagctcagca tcacggttgt gtgcatcgcg gcgggcaata cgatacgcca gacggttatc
    24421 acgcccctga tggtattgct ccagtatggc atcgagatac cgacagttgg cctctgtggc
    24481 gcgttcgagc atgcgcggca gattgcgaaa ctgccagtca ggccagatgt agctcactgc
    24541 cgcccacgca atggcacaac caatcagcgt atcgattacg cgaggtaacg ctacttcaaa
    24601 accttcaccc agtaagttaa aacacagtag caccaaaagt gtgatgaaca tcgttgcatg
    24661 agcgtattgc acgttacgga aggcaaaaaa gagcacgccg gtaataacca gcagcaccag
    24721 ctgcccttcc agtgatggca caaaccacag cacaggaatg ccaatggcga tacctaccag
    24781 cgtaccaata atccttaact tcaggcggtg gcgcgtggcg ttatagtttg gctggcagac
    24841 aaacaaactt gtcagcaaga tccaataccc gtgatgcatt ccggttatct gaatgatggc
    24901 gtagccgaag cacaacacca gcgacattct taccgcatga cggaagaggg cggattccgg
    24961 cgtgaagtga cggctaagac gcagccagat atcactcaac ccgtgcgggc tgtcatcagc
    25021 gagctcattt tcgtcattat tatggggtag tgcctgggcc tgttctgatt caattgttgc
    25081 cagttgggca tcaatggcgc gtaaattgtt cagcaaaaat cccagtgttt tgagtaaatc
    25141 ggcgggtgcg ccgttatcgc gcatccgctc cagcgcagca tcaatatgcg taaaagcgcg
    25201 ctcaaaatgc ggatcatgtt gataaggctg acgcaacaaa atacagcgtg acagttgctg
    25261 gcacgcctgg ccctgcatcg acatcagccg ctgaaaacgg aacagcacgt cgctgtggcg
    25321 aaaatgttca cgcaatgttt gatactgaat atgagaagag ctggcacgct cgtgaatatc
    25381 ctgtgcgaca aagtaataat gcagcgtgcg acgcgttccc cgttgaccac gatcgccacg
    25441 taagcgggtc agcagcgaga gtttcgtctg attcaatgtc gccatcagca gaccgttggc
    25501 gagagccaaa tcgtacagcg gtgcctggct ttgatcttca atatcaggat caaacatgcg
    25561 cgacttgagc tcaagataac gcgccagttg ttcatagcaa cgcgccaggt tgtcctgcag
    25621 cgggcggacc gggaacagca gatgaccaat aagtgtcagg acgttgtacc agacggcacc
    25681 ggccagcaga tacatcggct gctgatacca gtgctcatac agtgatgttc ccaacatagt
    25741 gtaaatggcg atcagcaatg caccgaaggc aattgttgca tagcgttgac ccagaccgcc
    25801 gagcaaaatg aagccgctgg tagagagcgt taagccaatc gcaaatagcc agggccaggg
    25861 aaacagcaat tctactgagg ccgaggcgat aaaaaagcag aacagcgtaa tgatgaggtt
    25921 acgcaaacgt cccgccagtc ggtcatcgag atcggtcagc gccgctgcca ccatccccag
    25981 cgttagcgga atcgtcagtt ttacatcacc cagccaccac ggaaacgctg tggttccaca
    26041 aagcgcaata aaaatacgcg cgtaatacag ccaggcgctg ttccaggtat agcgtttgag
    26101 caaaggactt agcataaagg ccatattagt ggattattca aaacgacgac gtgcattggc
    26161 ttcacgcgca gcttgcgctg tttctaccga taccacgcga cgaccaaccg gccatagtgc
    26221 aatagcggca attttaaagt tcgcaatgcc gacaggaatg ccaatgattg aaatacattg
    26281 tgcgatgccc gttgcaatgt gcatcaggca taaccaccag ccaaagaaaa tcagccagaa
    26341 aatattcaat accgtaccgc cagtattcag cagcacattt ttgccagccg ggttcagttc
    26401 atcgacatga atagcttcat tgccataagg caccagagac agtttagtga tctcccagca
    26461 ggatcgtgtc agcggtaagg taaaaatcag cacaatactg accagagtcg ccaacagcca
    26521 gcccagagtg gtggcaaatc cgccaagcac aaagttcaga atgttcaaaa cggtacgcat
    26581 aaaacctcgc tttactgtgg ttttcagtaa tgggcggcaa ttgtaacgtt tttttgggct
    26641 ggagcacgtt ttctctgacg gttacactga taagaaataa tttcgtgtgg atctacagag
    26701 tcatggaact gaaagcgaca acgcttggaa aacgtctggc acagcaccct tacgatcggg
    26761 cggtgatcct caatgccggg attaaagtct ccggcgatcg ccacgaatac cttattcctt
    26821 tcaatcaatt actggcgatt cactgtaagc gcggtctggt atggggcgag ctggaatttg
    26881 tactgccgga cgaaaaagtg gtgcgtctgc acggcaccga atggggcgag acgcagcgtt
    26941 tttaccatca tcttgatgct cactggcggc ggtggagtgg cgagatgagc gaaattgcgt
    27001 ctggtgtttt acgccagcaa ctggatttga ttgccacgcg cactggggaa aataaatggc
    27061 tgacgcgtga gcaaacctct ggcgtgcagc aacaaatccg ccaggctttg tcggcgttgc
    27121 cgttgccggt taaccgactg gaagaattcg ataactgccg tgaggcgtgg cgtaaatgtc
    27181 aggcctggtt gaaagatatt gaaagcgctc ggttgcagca taaccaggcg tataccgaag
    27241 ccatgcttac cgagtatgcg gattttttcc gccaggtcga gtcttcaccg ctgaatccgg
    27301 cgcaggcccg ggcagtcgtt aatggcgagc attctctgtt agtgctggca ggtgcaggaa
    27361 gcggaaaaac gtcggtgctg gtggcccgtg caggctggtt gctggcgcgt ggtgaagcgt
    27421 cccctgagca aattttattg ctggcgtttg gtcgcaaagc cgctgaagag atggacgagc
    27481 ggattcgcga acggctacat accgaagaca ttaccgcacg cacgtttcat gcgctggcgc
    27541 tgcatattat tcagcagggc agcaaaaaag ttccgatagt cagcaaactg gaaaatgata
    27601 ccgctgcccg tcatgaactc tttattgctg agtggcgcaa gcaatgcagc gaaaagaaag
    27661 cgcaggcgaa gggctggcgg caatggctga cggaagaaat gcagtggtca gtgccagaag
    27721 gtaacttctg ggatgatgaa aaattacagc gtcgccttgc ctctcgcctc gatcgctggg
    27781 taagtctgat gcggatgcac ggtggtgcac aggcagaaat gattgccagt gcacccgaag
    27841 agattcgcga tctgttcagt aaacgtatca agttgatggc cccgttatta aaagcctgga
    27901 aaggtgcgct gaaggcagaa aacgctgtcg atttttcggg ccttattcat caggcgattg
    27961 tgattctgga gaaaggtcgc tttatcagcc cgtggaagca tattctggtt gatgaatttc
    28021 aggatatctc gccgcagcgg gcagcgttgt tagcggcatt acgcaagcaa aacagtcaga
    28081 cgacgttgtt cgctgttggt gatgactggc aggcgattta ccgattcagc ggtgcgcaaa
    28141 tgtcgctcac caccgctttc catgaaaact ttggtgaagg cgaacgctgt gatttagaca
    28201 cgacttaccg ttttaacagt cgtatcggtg aggtggcaaa ccggtttatt cagcagaacc
    28261 caggccagct gaaaaagccg ctaaacagct taaccaatgg agacaaaaaa gccgtcacgt
    28321 tattggatga gagtcaactt gacgctttgc tggataagct ctctggttat gccaaaccgg
    28381 aagagcgcat tctgatcctg gcgcgttacc atcacatgag gcctgccagc ctggaaaaag
    28441 cggcaacacg ctggccgaag ttgcaaatcg actttatgac cattcatgcc agcaaagggc
    28501 aacaggcgga ttacgtcatc atcgttggct tgcaggaggg aagtgatggt tttccggctg
    28561 cggcgcggga gtcgattatg gaagaggcgc tactgccacc ggttgaggat ttcccggacg
    28621 ctgaagaacg gcggttaatg tacgtggcgc tgacccgggc acgccatcgg gtatgggcac
    28681 tgtttaacaa agagaatccc tctccctttg tggaaatact gaaaaatctg gatgtgccgg
    28741 tggcgagaaa accgtaagaa acaggtggcg tttgccacct gtgcaatatt acttcagacg
    28801 gtccgcgaga taacgctgat aatcggggat cagaatatcg accgcgtcgt tgaaatgcgg
    28861 cgactggatt atgaagtctg ccgttgccac gttggtggcg accggaatgt tccataccgt
    28921 cgccagacgc agcaaggctt tcacgtcagg atcgtgcggc acggcattta gtggatccca
    28981 gaagaaaatc aatacatcaa ttttcccttc tgagatcaat gcgccaacct gctggtcacc
    29041 ccccattggg ccactcaaca tcgcgttgac gttcatgccg gtcgcgcggg aaattaagtt
    29101 accggtagtg cctgttgcat acagtacgtg ttgttccagt aacggttgat gccgttccac
    29161 ccagctcatc agcatttgtt tgcagtgatc gtgtgccacc agcgcaatat gtttccgcgc
    29221 aggtaaagtg cgagtcgtca gttccataat gtacatccgt agttaacttt cctacagatt
    29281 actgtaagca cttatcgctg caagataaag accgaaaaag cctgcgcaca ggcacaaaaa
    29341 tctcaggaag atggttgttt ttccgcccac tgcaggaaag tatttcgcgt ttgtgggtca
    29401 gccagtttaa accaatactt cagccgttgt tctgtgagca cctgagactg cggtggaata
    29461 gcatccagct cggaaacacc cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat
    29521 tgcggtagtg aagcgcgttt cgcggatttg ttatagcgtt caacctcgac ctcataatca
    29581 atcgtttttg ccgttgaggt aatggcgaga atatccagct ttaccggaat cggcgtcgca
    29641 tcgccatcca acaattcaag gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc
    29701 tccaggcgag gcaggcgaaa attgacctgg ttgatgagct gggtattaaa actgaccacc
    29761 agcggtgggg agatatacag ccgttcttca ctattggaaa gatgaatcgt cttctcaacg
    29821 cgaaacacta actgatgcgg tccattatcc agttcaatgc tgtccgcacc gcgcagcaga
    29881 gagctggaaa ccttcttgcc gtcgagtacc agaagatcga catcggttga aagccgcagc
    29941 gtggtggcaa aaacggatac cggcaaacat aatgcaatca aggtggtcac gatgccggtt
    30001 ttcataggta gctcctgtca aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta
    30061 atcatactta ctaacatata gacatatttc ccgcttttgc tctcattcat tcgtattagc
    30121 tgcatggttg gcatgtcggc tttgtcgtac actttgttaa acagtctgga ggaaataatg
    30181 aaagaaaccg atattgcagg cattttaacg tccactcaca ccattgcgct ggtgggggca
    30241 agcgacaaac ccgatcgtcc cagctatcgc gtgatgaaat atctactcga ccaggggtat
    30301 cacgtcattc cggtttcgcc aaaagttgcc ggcaaaacgc tgctgggcca gaaggggtat
    30361 ggcacgctgg cagacgttcc ggaaaaagtc gatatggtcg atgttttccg taattcagaa
    30421 gcggcgtggg gcgtggcaca ggaagctatt gccattggcg cgaaaaccct ctggatgcaa
    30481 ctgggggtga ttaatgagca agcggcagta ctggcacggg atgccgggtt aaatgtggtg
    30541 atggatcgct gcccggctat tgagatccct cgcctgggcc tggccaaata aaaaatcccc
    30601 ggaaggcaaa aaccttccgg ggatttgttc agggattagt tacgcagacg cggggcctgg
    30661 agttgtttgc ggatggtctg cgccagctca tccatggatg gctgttcagg atgttcatcc
    30721 tgcaattcgc tacttagctg ggcttcagcc aggtaggtat gaaccggtag gccgttatcg
    30781 tcctccatca ctacgtgata ccacggagcg gcgcgaagct cgtcattcac cgccagctca
    30841 tcaggcgacg gttcagagag cgaataaacc gggtcgatat ccacgaccac tccgagataa
    30901 cctaacaggg aatggcggac ctgctggccg ataccgaatt tgctggcaat catagtcacc
    30961 tcccgggaaa tctttacctt tgatataagg gtaaaattcc acatttcaag ttacatgacg
    31021 cgacaggcaa accctttcag atatagccct tccggatagg tagcgatcac cggatgatcg
    31081 gctgcctgac ggaactgctc tataaattgt acatcacgac cggcatcaat tgcggcatcc
    31141 gcgatgattt tctgaaataa atcgctggtc atcagaccgg aacaggagaa agtcaggaga
    31201 ataccgcctt cattcagcag ctgaatcgcc agcatgttga tgtctttata accacgacac
    31261 gcgcccatca actggctttt attctcaaca aacttcggcg ggtccatcac gataacgtca
    31321 aatttttcac cgcgatcgcg ataagtacgc agcaatttaa agacatcatc acggacaaac
    31381 tcagccttgc tcagatccag tttgttcagc tcaacgttct gccgtgcaat atccagcgct
    31441 tcctgggagg tatcaacgct gacaacctgg ctgcaaccgc ccatcagtgc cgatacggcg
    31501 aaaccaccgg tataggagaa acagttcagc acacgtttat tttcaacgta gcggcgggta
    31561 gccaggcggc tatcacgctg gtccaggtag tagcccgttt tgtgtccgtg ctgaatatcc
    31621 accagcagtt tcattccgtg ttcttcaatc ggcagcaggg caggtggcaa ctcgccggtg
    31681 acggggccct gggtcagctc cattccttct tttttacgta ccgcgacgtc gctgcgatcg
    31741 taaatcgaac attccgggta cagcgtttgc agggcactaa ttaatgccgc gcgctgatat
    31801 tctgccccag cactcagcag ttgcagcacc agaaaattac cgaaacgatc gatagtaata
    31861 cccggcaggc catcagattc tccggcgatt aaacgatagc tgtcgaggcc atctttttgc
    31921 gccagccagt cacgccattt ttgtgcttgt tgcaaacggc gggaaaaaaa gcaatgtcga
    31981 tagactcaga cgggtcaaac gtccagacgc gcgcccggat ttgcgaagct ggcgaataag
    32041 cgccgcgtgc taaccatttt ccctgatgat caacaatatc gatggtttca ccgaggctgg
    32101 ctttaccttc catgcgggca acggccccgg aaaagaccca cggatggcga cgaagtaatg
    32161 atttttcgcg ccctttggct aacactaaac gtacactcat aatttacttt tctgtcgatg
    32221 caaagaaatg ggcgtcattg tccggagttt tccatggatt tgcaacgcgt ccattaagga
    32281 taaggagagc agcaatgtcg aaagtctgca taattgcctg ggtttacggg cgagttcagg
    32341 gcgtaggatt tcgctacacc acacagtacg aagcgaaaag actgggttta accgggtacg
    32401 ccaaaaatct tgacgatggc agcgtggaag tggttgcttg cggtgaagaa gggcaggtgg
    32461 aaaaattaat gcagtggtta aagagtggcg gcccgcgttc tgcgcgtgta gaacgggtgc
    32521 tcagcgagcc gcatcatccc tcgggggaat taaccgattt tcgtatccgt taaatacatt
    32581 ttaccggttt tggcaggcca gcaattttgg tggcttgctt agccggacct ttcggaaaca
    32641 gtcggtataa atagcggctg ttaccttttt cttcgccaaa tttattcgcc atcgctttta
    32701 ccagcatacg aatcgccgga gaagtattga attccagata gaaatcacgc acaaaacgca
    32761 ccacttccca gtgttccggt gacagcgaaa tcccttcgtt ctctgcaatc accacagcca
    32821 gcggctcact ccactggctg ctttctttga gatagccttc ggtatccgtt tctatctctt
    32881 taccttcgaa gatcagcata attattacta ccttaatcag actggcggca gtgtaacaaa
    32941 aaacaaagcc ccgcataaag cgaggctatg aaagtgttag cgggtgagat taatcgcggc
    33001 tagcgaagcc cagaatgctc agcaggctga cgaagatgtt gtacagcgaa acatacaggc
    33061 taaccgtggc acgaatatag ttcgtctcac cgccatgaat gatgttgctg gtttcaaaca
    33121 agatagcgcc agaggagatc agaatgaaga ccgcgctgat cgccagatgc agagcaggca
    33181 gctgcaggaa gatattcgca accataccaa tcagcaccac cacaataccc gccatcagca
    33241 taccgccgag gaacgacata tctttgcggg tggtcagcac atatgcagag cagcagaaga
    33301 acactaacgc cgttccgccc agtgccatag cgattacgtc acccattccg gcagacagat
    33361 aggtgttcag aataggtccg aggatataac ccagaaaacc ggtaaaggcg aatgcggaga
    33421 taatcccggt cggcttattc gccgttttat aggtcaggaa catcaaacca tacataccca
    33481 ccagcgtcag aatcagaccc ggagatggca gcatcagcac cgtactggca gttgcggtaa
    33541 tcgccgaaaa ggccagcgtc aggctcagca gaaaataggt attacgcagc accttatggg
    33601 tgctaagcag tgatgtacgg tcatgtgaag aactaacaat acgatccatt gagtcactct
    33661 ctatgacaga tgtaattaat taagcagcat aatgataatg cgtaagggca cccagaagtt
    33721 ttacccatct ttacgcattt gatctggaac aggtttaaca gcggattatc aggtcattaa
    33781 gcaaatataa cgccctgaga atttcgacag gcaaaagaaa aaggggttag catttagcta
    33841 accccttatc ttatttggcg gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc
    33901 cggttttcaa gaccggtgcc ttcaaccgct cggccacact tccggaatga cgcgcactat
    33961 aaacatcccg atgcggcgtg taaaccccta atttgtttgt ttgcctgaaa aacagccaaa
    34021 agtgcattga tagcgtgaaa taacagcaga ttgatcattt catcaccatg aattccttct
    34081 cttttactcg tttagcaacc ggctaaacat ccccaccgcc cggccaaaag aaaaataggt
    34141 ccatttttat cgctaaaaga taaatccaca cagtttgtat tgttttgtgc aaaagtttca
    34201 ctacgcttta ttaacaatac tttctggcga cgtgcgccag tgcagaagga tgagctttcg
    34261 ttttcagcat ctcacgtgaa gcgatggttt gccttgctac agggacgtcg cttgccgacc
    34321 ataagcgccc ggtgtcctgc cggtgtcgca aggaggagag acgtgcgata tgaataacga
    34381 ggaaacattt taccaggcca tgcggcgtca gggcgttacc cggcgcagct ttctcaaata
    34441 ttgtagtctg gctgccacgt cgctgggatt aggcgcggga atggcaccaa agattgcctg
    34501 ggcgctggag aacaaaccgc gcattccggt ggtatggatc cacggtctgg aatgcacctg
    34561 ctgtaccgaa tcttttatcc gctccgctca cccactggcg aaggacgtca tcctttccct
    34621 gatttccctc gattacgacg atactttgat ggctgccgcc ggaacccagg cggaagaagt
    34681 ctttgaagac atcatcacgc aatacaatgg caaatatatc ctcgcagtag aaggtaatcc
    34741 gccgctgggc gagcagggga tgttctgtat cagcagcggt cgaccgttta ttgagaaact
    34801 caaacgtgcc gctgccggag ccagcgcgat tatcgcctgg ggaacctgcg cgtcctgggg
    34861 ctgcgtgcag gccgcgcgac ccaatccgac gcaggcaacg cctatcgaca aagtcatcac
    34921 cgacaaaccc attatcaaag tacctggctg cccgccgatc ccggatgtga tgagcgccat
    34981 cattacttac atggtgacct ttgatcgctt gccagatgtc gacagaatgg gccgtccgct
    35041 gatgttctat ggtcagcgaa tccacgataa atgctatcgc cgcgcccact tcgacgccgg
    35101 agagttcgtc cagagttggg atgatgacgc tgcccgcaaa ggttactgcc tgtacaaaat
    35161 gggctgcaaa gggcctacca cctataacgc ctgttcctcc acacgctgga atgatggcgt
    35221 ttctttccca atccagtctg gtcacggctg cctgggctgt gcggaaaatg gtttctggga
    35281 tcgcggttcg ttctacagcc gcgtggtcga tattccgcaa atgggtactc attccaccgc
    35341 cgataccgtc ggtttaaccg cgcttggcgt ggtggcagcg gctgttggtg tgcacgcagt
    35401 cgccagcgcc gttgaccagc gcagacgtca taaccagcaa cctacagaaa ccgaacatca
    35461 gccaggcaat gaggataaac aggcatgagc actcagtacg aaactcaggg atacaccatc
    35521 aataatgccg gacgccgcct ggtggtcgac ccgattacgc gcatcgaagg ccacatgcgc
    35581 tgcgaagtga atattaacga tcagaatgtg atcaccaatg ccgtctcctg cggcaccatg
    35641 tttcgcgggc tggagatcat cctacaaggg cgcgacccgc gcgatgcgtg ggcgttcgtt
    35701 gaacgtatct gcggcgtctg tactggcgta cacgccctgg cttcggttta cgccatcgaa
    35761 gatgctatcg gtattaaagt gccggacaac gccaatatca tccgcaacat tatgctggca
    35821 acgctctggt gccacgatca tctggtgcac ttctatcagc ttgccgggat ggactggatc
    35881 gatgtgttag atgcgctgaa agccgacccg cggaaaacct ccgaactggc gcaaagtctc
    35941 tcctcttggc cgaaatcatc ccctggctat ttcttcgacg tacaaaaccg cctgaaaaaa
    36001 tttgttgaag gcgggcagtt ggggatcttc cgcaatggct actgggggca cccgcagtac
    36061 aaactgccgc cagaagctaa cctgatgggc tttgcccact atctcgaagc tctcgatttc
    36121 cagcgtgaaa ttgtcaaaat ccacgcggtc tttggcggta aaaacccgca tccaaactgg
    36181 attgtcggcg ggatgccttg cgccatcaac attgacgaaa gcggcgcggt cggggcagtc
    36241 aatatggaac gcctgaacct ggtgcagtca attatcaccc gcacggcgga cttcattaac
    36301 aacgtgatga tccccgacgc cttagccatc ggtcagttca acaaaccgtg gagcgaaatc
    36361 ggcactggtc tttctgataa atgcgttctc agctacggcg cattcccgga tattgccaac
    36421 gactttggcg agaaaagtct gctgatgcct ggcggcgcgg tgattaacgg cgacttcaac
    36481 aatgtgctgc cagtggattt ggttgatccg cagcaggtgc aggagtttgt cgaccacgcc
    36541 tggtatcgat atcccaacga tcaggtcggg cgtcatccgt tcgatggcat caccgacccg
    36601 tggtacaacc ccggcgatgt caaaggcagc gataccaaca ttcagcagct gaatgaacag
    36661 gaacgctact cgtggatcaa agcgccacgc tggcgcggta acgcgatgga agtggggccg
    36721 ctggcgcgca cgttaatcgc ttatcacaaa ggcgatgctg cgaccgttga gtcggtcgat
    36781 cgcatgatgt cggcgttgaa cctgccgctt tccggtatcc agtcaacgtt aggccgcatt
    36841 ttgtgccgcg cgcacgaagc gcagtgggcc gcaggtaagt tgcagtattt cttcgacaag
    36901 ctgatgacca acctgaaaaa cggcaatctc gccactgctt ccacggaaaa atgggaacct
    36961 gcaacctggc cgacagagtg ccgtggtgtc ggttttaccg aagcgccgcg cggggcgtta
    37021 ggccactggg ccgccattcg cgatggcaag attgatctct accagtgcgt ggtgccgacc
    37081 acctggaacg ccagcccgcg cgatcccaaa gggcagattg gcgcttatga agcggcgctg
    37141 atgaacacca aaatggcgat ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc
    37201 tttgacccgt gcctcgcctg ttcaacacac gtgctgggcg acgacggtag cgagctgatc
    37261 tccgtgcagg tgcgttaaca gcgaaggaga atcatcatgc aacagaaaag cgacaacgtt
    37321 gtcagccact atgtctttga agcgccagtg cgcatctggc actggttgac ggtgttatgc
    37381 atggcggtgt tgatggtcac cggatacttt atcggcaagc cgctaccttc cgtcagcggc
    37441 gaggcgacgt atctgttcta tatgggctac atcaggttaa ttcacttcag cgccgggatg
    37501 gtttttaccg tggttttgct gatgcggatc tactgggctt ttgttggcaa tcgatactcc
    37561 cgcgagctgt ttatcgtgcc ggtatggcgt aaaagctggt ggcagggcgt gtggtatgaa
    37621 atccgctggt atctgtttct ggcaaaacgt ccgagtgccg atataggcca taatcccatc
    37681 gcccaggcgg cgatgttcgg ctatttcctg atgtcggtct ttatgatcat cactggtttt
    37741 gcgctgtaca gcgaacacag ccagtacgct atttttgcgc cgttccgtta tgtggtggaa
    37801 tttttctact ggacgggtgg caactcaatg gacattcaca gctggcatcg gctggggatg
    37861 tggctgattg gcgcgtttgt gatcggtcat gtctacatgg cgctgcgtga agacatcatg
    37921 tccgacgaca cggtgatctc caccatggtc aacggctacc gtagccacaa atttggcaaa
    37981 ataagtaaca aggagcgttc atgagcgagc aacgcgtggt ggtcatgggg ctgggcaacc
    38041 tgctgtgggc cgatgaaggc ttcggcgtgc gggtggcgga acggctgtat gcccattacc
    38101 actggcccga gtatgtggag attgtcgatg gcggtactca gggactgaac ttgctggggt
    38161 atgtcgaaag cgccagccat ctgttgattc tcgatgccat tgactacggg ctggaacctg
    38221 gaacgctgcg aacctatgcc ggagaacgca ttccggctta tctcagcgcg aagaaaatga
    38281 gcctgcatca gaacagtttc tccgaagtgt tggcgctggc ggatatccgc ggacatctgc
    38341 cagcacatat tgccctcgtc ggtctgcaac ccgcaatgct cgacgactac ggcggtagcc
    38401 tgagcgaact ggcacgggag caactgcccg ctgcggaaca ggcggcgctg gcgcagcttg
    38461 ctgcgtgggg aattgtgccg caaccggcta atgaatcgcg ctgtctcaat tatgactgtc
    38521 tgtcgatgga aaattacgaa ggcgttcgct tgcgccagta ccggatgaca caggaggagc
    38581 agggatgagc aacgacacgc catttgatgc gttgtggcaa cgaatgctgg cgcgcggctg
    38641 gacgccagtc agtgaatccc gtcttgacga ctggcttacg caagcgccag acggcgtggt
    38701 gttattaagc agtgacccga aacgcacgcc agaggtcagc gataatccgg taatgattgg
    38761 cgaattactg cgcgagtttc ccgactatac atggcaggtg gcgattgctg accttgagca
    38821 gagcgaagcc atcggcgatc gctttggcgt ctttcgcttt cctgccactt tagtgtttac
    38881 cggcggaaac tatcgcggcg tgctgaatgg tattcacccg tgggcggaac tgataaacct
    38941 gatgcgcggg cttgtcgaac cgcagcagga gcgtgcctca tgagcgaaac ttttttccat
    39001 ctgctggggc caggaacgca accgaacgat gacagtttca gcatgaatcc actgccgatc
    39061 acctgtcagg tgaatgatga accgagtatg gcggccctgg agcaatgtgc tcacagcccg
    39121 caggtgattg cgctgttaaa cgagttacaa catcaactaa gcgaacgcca accgccgttg
    39181 ggcgaggtgc tggcagtcga tctgttaaat ctcaacgccg acgatcgtca ctttatcaat
    39241 acgcttctcg gggaagggga agtgtcagtg cgcattcagc aggctgacga cagtgaaagt
    39301 gaaatacagg aggcgatctt ctgcggatta tggcgggtgc gcagacgtcg cggcgaaaag
    39361 ttgctggagg acaaactgga ggctggctgc gcgccgctgg cgttgtggca ggcggcaacg
    39421 caaaatctct tgccgacaga ttcgctgtta ccgccgccca ttgatggcct gatgaatggc
    39481 ctaccgttgg cgcatgagtt actggcacat gtacgtaacc ccgacgcgca gccgcacagc
    39541 attaatctga cgcaattacc catcagcgag gctgatcggc tttttctctc acgtctctgt
    39601 gggccgggaa atattcagat tcgtaccatt ggctatggcg agagctatat caacgccacg
    39661 gggttacgcc atgtctggca tttacgctgt acggacacct taaaaggccc gttactggaa
    39721 agttatgaaa tctgcccaat accggaagtg gtgctggcag cgccagaaga tttggtcgac
    39781 tctgcgcagc ggcttagcga ggtatgtcag tggctggcgg aagctgcacc gacgtaaaaa
    39841 gacggtaagt atcgctttca gtcttatgaa tatcgcaatc ggcgaatacc tctggtcgta
    39901 gagtttcagg ataaagaggg agatctacca ttatcgggtt atttttctct cttcgcctac
    39961 aggagtgcgc atgtgggatg tcattgattt atcgcgctgg cagtttgctc tgaccgcgct
    40021 gtatcacttt ttatttgtac cccttaccct ggggctgatt tttttgctgg ctattatgga
    40081 aaccatttac gtggtcaccg gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa
    40141 gctcttcggt atcaattttg ctcttggcgt ggctaccggc ctgaccatgg agtttcagtt
    40201 tggtactaac tggtcattct attccaacta tgtgggcgat atttttggcg caccgctggc
    40261 gatggaagca ttaatggcct tcttcctcga atccaccttt gtcgggctgt tcttcttcgg
    40321 ctggcaacgg ctgaataaat accagcacct gctggtgacg tggctggtgg cgttcggttc
    40381 aaatctctct gcgttgtgga tattgaatgc caacggttgg atgcaatacc cgaccggtgc
    40441 gcattttgat atcgacaccc tgcgtatgga gatgaccagc ttcagcgagc tggtctttaa
    40501 tccggtcagc caggtgaaat ttgtgcacac cgtaatggcg ggctacgtga ccggggccat
    40561 gtttattatg gcgatcagcg cctggtattt actgcgcgga cgggagcgcg atgtcgcatt
    40621 acgctcgttt gccatcggtt ccgtcttcgg tactctggcg attatcggta ccctgcaact
    40681 cggagacagt tctgcgtatg aagtcgcgca agtacaaccg gtaaaactgg cggcgatgga
    40741 aggggagtgg caaacggaac ctgcacctgc accgttccat gtggttgcct ggccggaaca
    40801 ggatcaagag cgtaacgcct ttgccctcaa aattcccgcg ctgctaggga tcctcgccac
    40861 tcactcatta gataaacccg tgccgggtct gaagaatttg atggctgaaa cctacccacg
    40921 cttgcaacgc ggacgtatgg cctggctgtt aatgcaggaa atatcgcaag gcaatcgtga
    40981 gccgcatgtg ttgcaggcat tccggggact ggaaggtgac ctgggctacg gcatgttgct
    41041 ctcccgctat gcgccggata tgaatcatgt cacagccgca cagtaccagg cggcgatgcg
    41101 tggcgcgata cctcaggttg cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg
    41161 ttccctgctg ctactggtga tgctgattgc gcttgtccag acgctgcgtg gcaaaatcga
    41221 ccagcatcgc tgggtgctga aaatggcgct ctggagtttg ccgttgccgt ggattgcgat
    41281 tgaagccggg tggtttatga ccgagtttgg tcgtcagccg tgggcgatac aggacatctt
    41341 accgacatac tccgcgcact ccgctttaac cacaggacaa ctggctttct cactgatcat
    41401 gatcgtaggg ctttacaccc tgttcttaat cgccgaagtc tacctgatgc agaaatatgc
    41461 ccgtctgggg ccgagcgcga tgcagagtga acaaccgacg cagcaacagg ggtaaaggag
    41521 aaaatcatgt ttgattatga aacattgcgc ttcatctggt ggctgctgat tggcgtgatc
    41581 ctggtggtct ttatgatctc cgacggattt gacatgggga tcggctgtct gctgccgctg
    41641 gtggcgcgta atgatgatga acgccggata gtgataaaca gcgttggtgc acactgggaa
    41701 ggcaaccagg tctggttgat cctcgctggt ggggcattat ttgccgcctg gcccagagtg
    41761 tatgcagcgg cgttttccgg cttttatgtg gcgatgatcc tggtgctgtg ctcactgttc
    41821 ttccgcccgc tggcctttga ttatcgcgga aaaatcgccg atgcacgctg gcgtaaaatg
    41881 tgggacgccg gtctggtcat cggcagtctg gtgccgccgg tagtcttcgg tatcgccttc
    41941 ggcaacttgt tgctcggcgt gccgtttgcc ttcacaccgc aattacgcgt ggagtatctc
    42001 ggcagcttct ggcaactgct gacgccattc cctttattgt gcggattgct cagccttggg
    42061 atggtgattt tgcaaggtgg cgtctggtta caactgaaaa ctgttggtgt gattcatctg
    42121 cgttcacagc tggcgaccaa acgcgctgca ctgttggtga tgctgtgctt tttgctggcg
    42181 ggttactggc tgtgggtcgg tattgatggc tttgtactgc tcgcccagga tgctaacggt
    42241 ccttccaatc cgttaatgaa actggtggca gtgctacctg gtgcctggat gaataatttt
    42301 gtcgagtcgc ccgttttgtg gatcttcccg ctgctgggat tcttctgccc attgctgacg
    42361 gtgatggcga tttatcgtgg tcgcccgggt tggggatttt tgatggcatc attgatgcaa
    42421 tttggcgtga ttttcacggc aggcatcacg ctgttcccct ttgtcatgcc gtcaagcgtg
    42481 agtccgatct ccagcctgac gttgtgggac agtacttcca gtcagctgac gctgagcatt
    42541 atgttggtaa tcgtgctgat atttttgccc attgtgttgc tctacactct ctggagctac
    42601 tacaaaatgt gggggcgcat gacaacagaa actctccgcc gtaacgaaaa cgagttgtac
    42661 taaggagcag aaacaatgtg gtatttactt tggttcgtcg gcattttgtt gatgtgttcg
    42721 ctctccaccc ttgtgttggt atggctggac ccgcgtctga aaagttaacg aacgtaggcc
    42781 tgatgcggcg cattagcatc gcatcaggca atcaataatg tcagatatga aaagcggaaa
    42841 catatcgatg aaagcgatct taatcccatt tttatctctt ctgattccgt taaccccgca
    42901 atctgcattc gctcagagtg agccggagct gaagctggaa agtgtggtga ttgtcagtcg
    42961 tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg caggatgtca ccccagacgc
    43021 atggccaacc tggccggtaa aactgggttg gctgacaccg cgcggtggtg agctaatcgc
    43081 ctatctcgga cattaccaac gccagcgtct ggtagccgac ggattgctgg cgaaaaaggg
    43141 ctgcccgcag tctggtcagg tcgcgattat tgctgatgtc gacgagcgta cccgtaaaac
    43201 aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca ataaccgtac atacccaggc
    43261 agatacgtcc agtcccgatc cgttatttaa tcctctaaaa actggcgttt gccaactgga
    43321 taacgcgaac gtgactgacg cgatcctcag cagggcagga gggtcaattg ctgactttac
    43381 cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg cttaattttc cgcaatcaaa
    43441 cttgtgcctt aaacgtgaga aacaggacga aagctgttca ttaacgcagg cattaccatc
    43501 ggaactcaag gtgagcgccg acaatgtctc attaaccggt gcggtaagcc tcgcatcaat
    43561 gctgacggag atatttctcc tgcaacaagc acagggaatg ccggagccgg ggtggggaag
    43621 gatcaccgat tcacaccagt ggaacacctt gctaagtttg cataacgcgc aattttattt
    43681 gctacaacgc acgccagagg ttgcccgcag ccgcgccacc ccgttattag atttgatcaa
    43741 gacagcgttg acgccccatc caccgcaaaa acaggcgtat ggtgtgacat tacccacttc
    43801 agtgctgttt atcgccggac acgatactaa tctggcaaat ctcggcggcg cactggagct
    43861 caactggacg cttcccggtc agccggataa cacgccgcca ggtggtgaac tggtgtttga
    43921 acgctggcgt cggctaagcg ataacagcca gtggattcag gtttcgctgg tcttccagac
    43981 tttacagcag atgcgtgata aaacgccgct gtcattaaat acgccgcccg gagaggtgaa
    44041 actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc atgtgttcgt tggcaggttt
    44101 tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt ttgtaatgca taaaaaagag
    44161 cattcagtta cctgaatgct ctgaggctga tgacaaacga agaactgtct aatgcgtaga
    44221 ccggaaaagg cgttcacgcc gcatccggcc actttcagtt ttactctttc tcggagtaac
    44281 tataaccgta atagttatag ccgtaactgt aagcggtgct ggcgcgttta atcacaccat
    44341 tgaggatagc gcctttaata ttgacgcctg cctgttccag acgctgcatt gacaaactca
    44401 cctctttggc ggtgttcaag ccaaaacgcg caaccagcag gctggtgcca acagaacgcc
    44461 ccacgaccgc ggcatcactc accgccagca tcggcggcgt atcgacaatc accagatcgt
    44521 aatggtcgtt cgcccattcc agtaattgac gcatccgatc gcgcatcagc agttcagacg
    44581 ggttaggtgg cacctgaccg cgagtaatca catcaaagcc tcctttgcca aaatgctgga
    44641 tcactttgtt gagctcatct ttacctgcca gatattccga caagccatgt tcattactca
    44701 cggtaaacag gttatgcgaa taaccacggc gtaagtcggc atcaataaat aacacttttt
    44761 gatcggactg ggcgatcacc gctgccagag ttgaactgac aaacgtttta ccactgtctg
    44821 gcgtcgcacc ggtgatcatt agaatgttat tctccgtctc catcatagcg aaatgcagac
    44881 tggttcgtag cgcacgtacg gcttccacag cagaatccgc cgggttatcc accgccagga
    44941 aggggatatt tttagtacga tggcgctgct gattagaaaa taaatttttc ttacgcagac
    45001 gggtgcgttt atccagccac tcggacattg ggatagtggc ataaacgctg atgccgtgct
    45061 cttccagttg ttccggggct tctacaccac gacgcaacat cgcacgcgcc agcacggcac
    45121 ccacagaaat aaacaggcca agaataaaac caagcaccac gttcaacgct tttttcggtt
    45181 tcactggctg cggctgagtg actgccgggt cgataatccg cacgttacca atggcactgg
    45241 atttcgaaat actcaactcc tgctggcggt taagtaattg cagatatacc gcacggcccg
    45301 cttctacgtc acgacttaaa cgcaacactt cctgttgggt ggaaggcatt gccgataccc
    45361 gcttattcag gcgtttgcgt tcttgctcca gcgtctggcg tttttccagc agcgcacgat
    45421 aagttgggtg atctttctta tacagctggg agatctctgc ctcgcggaag gtcagctcat
    45481 tgagttgatt atcaacgttc acaatctgct caagaacggc tttggcttcc aggttaaggt
    45541 caaccgaatc gcgctgctgg cgataaacgt tgagtttttc ttccgcttgg tccagctcgc
    45601 tgcgcacttc aggtaactgg cgctgtaaga attcaaggct ttgtgaatcc tgcgccgcct
    45661 ggcgagcgat attctgttgc aaatagttgt tagcgatgct gttcagaata cgagtaatca
    45721 actggggatc atcaccagtc atggtaagtt ccagcatccc gctttcttta ctgcgttcgc
    45781 taacggtaaa ggtttcctgc aatgcgttaa tcgcttccag ttcggtacgc tggctcagga
    45841 caaactgtgt tcctggtttg gccttaatgt ccgcgatagt cagcgcaacg ccatcttttt
    45901 ccagacgctg gccgaccata ccattgacgg tgaactcttc accttccagt gtatagtggc
    45961 cgttttcccc aaccgtgagt gtcagttgct gatcctgacc attcagttgt ggaatatgca
    46021 tccagctgat cgccagctca cctggttttt ctttggttaa tctcgcccag ccgcgaccca
    46081 caatcggaaa atacttctgc tcaactatgt cgcgcagatt cagttcagca atggttttac
    46141 cgagaatcat gcgcgattgc agcagttgga tctccggtgc agactcgggc gatgagttag
    46201 ggatcatatc gctcaggccg ctgagaatgg cgttgccctg tttttgctca acctggacca
    46261 gagtatctgc ctgataaatt ggtgtgctta acagcgagta agcgacagcg atcagcgtga
    46321 ataacgcggt cacgctgata ataaacttac ggtgatccca taactcgccg accagacgaa
    46381 gcagatcgat ctcattttcc tgagtgctgc ctggtggcgt attcatattt ttagttgtca
    46441 tcgtatttta taaactcaaa ttaccggctg aggcgcttcg cccattcctg actggcgcgc
    46501 tccaacatac cgtagacatg ttcaaatgcg tcctgacttt tacgataggg atccgggatc
    46561 tctttctgtt ccagccattg cccaaacagc attgtcttcc cgcggacctc gggagcaatt
    46621 gcggtaacct gagcaatatg ttccgactcc attgccagaa tcagatcgta atttcgtgcc
    46681 atctcagcag tgagcttacg tccggcatgt ccttcaagag atacgccatg attagcggca
    46741 acatctgccg ctgtcgcatc tgcagggtgt tttaccagac catgaacacc tgccgatttg
    46801 acttttacgc ccggcagacg tttacgcagc aagcgctcgc caatgggcga acggcaaatg
    46861 ttgccggtac aaaccaccag gattgagtta aattttagtt gggccatgtt tgaatccacc
    46921 gtgaagtctc tgtcaggtca tgcaccccgg aaatcgtcgg taccagcaga gaaatcacac
    46981 ggttccaacg agccagcggc gcggtagtga cataaacaat gtcgtaaggt tcaagctgga
    47041 attccgttcc gagtatcatt gccgatgcgt ccttcgcatt cagctgataa atattggcga
    47101 ttttgccatt ctgcttgttt tgcgttgcac gaataacaaa aatccccgtc gcatcagcga
    47161 catcctgatt cataccctca gcgttaccca gcgcttccgc cagggtcata ccgctgcggt
    47221 ccattttcag cgtgctctgt ttacccactt cacccatcac gaagactttc agatcatcgt
    47281 tgcgcggaat aaataaaata tcgcccgggt gcagcagttt gttctggcgc aagtcgccac
    47341 gctgcatcag cgcatagagg ttcactttgg tctttacgcc attctgggtg agcaccacgt
    47401 tgcgccagtc tgcatctgcg gtcagaccgc ccgcggcgtt aatcgcatcc atgatggtca
    47461 gaggaatatt ggtaatcggc tgctgacccg atttgctgac ttcgccggtg acgtacgctt
    47521 tttgcgatcg gaacgccgcg acgctgacat cgacttgcgg gctttcaatg actgaatcga
    47581 gacgcgctgt aatctcgttg cgtacctggg taagtgtttt cccggccact ttcaggcgac
    47641 caatataggg gtagaaaatc gcaccatcgg cgttaaccca gttgccggta tcactggcgc
    47701 tacggtattg ccctgcgggg gtggttagtt ccgggtgatc ccagaccgta accatcaaca
    47761 catcaccaat cccgatacga tattcatagt ttgcgatttg ctgctccagc tccgggttag
    47821 cctgcgacat aatcggtttg gcgcgcaact gatcaatcaa tcctggcgtg acgggataga
    47881 tattgaccat tttgtccagg tcatactgat tgtcaggtag ttcaatgaca tccttgttac
    47941 tggtcgataa attctgacca ggaacaaggg tacatgctgt caaagacaga acagccaatg
    48001 tcagtaccga aaacttaaaa atgttctttt tcattggttt atatttatca gtcaatgtaa
    48061 tgttatgaat tacgtcaggt tattgtccaa ccggtacttc tcgctctgcc gtcattggat
    48121 agagctggta tttgcgtgac agcatttgtc caccatcacg cgtcaatggt gtccacgaaa
    48181 ccaccgcgcg gttgcggtta ggtccaatgg tcatcaagtc gaatggaatc gagatataaa
    48241 aacctttact aaagccgcct tcgccgtagt catctttaga tacgttactg attgccgccc
    48301 atacccctac cgccacgccg ctgtcaaagc gtttggcgac gtcgatcgtt gcgcctttat
    48361 cttttgccag atattgccca acgctaagtt tcatcagtac gccattgagc gtcggcgggt
    48421 tccagtaagc cgtcacgaaa cctgttggcg tggaataatc ggtgaaacgc atcatgttgt
    48481 cccagtcacg ttgcttcacg tagttaacgt ccacacccag cgcccagcag gcatctagcg
    48541 ggcgataaag cagctcggaa ccgacaccgg cgtacatcgt ttccagataa ccgccataca
    48601 cctggccata gaaaccattg cctaagtcag caaaatagtt cgcctgcaag ttgttgagat
    48661 aaacgtcatt gcgaacgtag tcacggatat gcgtgcgcac gcggggcagg gtagagtccg
    48721 cgggcaacag cgaagactta aacttgtcgt agttgttgta aatattggtg aaaataccgc
    48781 catcaagcag caggtggtcg gtaaaccagt agcgggcact ggacatcagc cccagctgga
    48841 acatatagaa atcttccggg ccgcccagcg actgtgaaag tgttgggttg aaagagtagc
    48901 taaagcgatc ttcacgaata cggtagcccc gaccaaaggc agaaagatct tcagcttcaa
    48961 cacgttgttg ttgcagtggc tctgattgac caggcgctgt acctgccagc tgtttgcgca
    49021 ggctggctac gtcggtttcg gtagtcacca tcgccatatg ctcgcgcttt tgcgtcacgc
    49081 taatcttctc aacgccttgc ggcaggttat tcaccagaat ccggttggca cgatcgaccg
    49141 cttcacgaga gtcacggtat ttgtattgct gaccagacat atacagtgtc ttatcgcgta
    49201 gctgaatttc tggcgcgtca aagcccgcgt tatacttcag ggcggtaagt tggtttgcta
    49261 ccgtggtgta ctgcaatcct tcagattcag gcgcaggttg atatgccggt tttggcgtat
    49321 cgcgcagggc agggcgcaga tcgttgaaat tggtccgtaa cgtgaagcca aacatcaacg
    49381 tgttaccgcg ttcataactc aggttgagat ctgcccagct ggcagcgcga taaactgcgc
    49441 cgacgttgaa atggcttgcc tgaggcagtt tgccagcgaa atcattctgg taattgttgc
    49501 catcgtattc gagtttcaga cgcaggggat tccacggcgt ttgatactca atgccgccaa
    49561 agatggaagc cgggccacga aagatatcgc taaagctgat atcgcccgca tcgtgagact
    49621 ctgcgcgatg acaatattta tcgctcaccc ggcaaaacgg gttggtaata ttgcccgcat
    49681 taccggcgta gccccatgcc atcccgaggg tgaaatcaaa tggccccgct tgcttgctgg
    49741 ccaccagata ctcaccgtca aacagacccg taccagcaat atcacgttta ccaaacgcca
    49801 cttgcggtag ccagtaacct tcttcccaca ggcgaagttt aaaatcgaat gatttgtctt
    49861 tgtatgactg atcgccgctg aaatcttccc actggctata tttgcgtgtg cgcacatccg
    49921 tataacgaat ggtgccttcc agccatggga acagcgccac ggaggtggag tagaaccggt
    49981 attgatcgtt atcccggtag ttgacgctga attcaccttc cggtgcgatg cgcgcatttg
    50041 gcatctgcaa caatcctgtg ccgccaaagt ctgattgcga cgaacccagc ggatccgggt
    50101 aggttaatac ttcagcatga caggcactgg agacggcaat ggccaggcag cttaaaagat
    50161 aagaattctt cttcatcagt caggaacacg ttgcgttaaa acagacacaa tctggtcatt
    50221 taaatcagca tatttttcgg gcagtacatg cgcagaaaag cccaaccaaa gctggctgcc
    50281 aggcggtggt tccacatgtc ttttattcca cagggcaaca ggggcgacga cagtctcacc
    50341 ctcaggcgta atcaccataa cgttattctt gtccgcgcct gcaaggcggg ggtgatcttg
    50401 cagataatcg gtaacgctac ggcctgcctg ccagggcaat tgcccagccc cagaaaccgc
    50461 acccagcaga gtaatagtca ccggacgctg tacggtatag agcgtatagt ccccgaccaa
    50521 cgggggattg ctgttctcat ccactcgtac aaaatcaggg tccagtttga ccggtaagcg
    50581 accagtgata ttgagattga gtaactgctg gcgcacggat ttaatcgttg ccgccacatc
    50641 gtcgtcagct tctgcttccc aggaagcaag ctgtgccatg acatgttggt agtctttaag
    50701 cgctttagct ttcgccgcag agtcggtcag caaggcacca ggccaccaga ggcgatcgcg
    50761 cagttgcggt tgtgtcacca actggacaac attttccacg gggccaacgg aaagtgtctg
    50821 ttgttcgcca ggcagataaa tagtcaccgt tccttgcgca aaggcatggg gtgtcattac
    50881 gtaaagtacg ctggcaatga aatacgactg taatttattc atggtgcggg tttaagaaac
    50941 gtcatttcta cgggaataac ccctgcgcct aacatttgcc gactttgacg aacctggcca
    51001 gtagccgaat cgacccagaa tgtgttttgc cactgttttt ctggccgcgt ggaagtcacc
    51061 tcttcctgcc agacattaca cagcacattg cgacccgcaa tattcagcgt ctctaatcca
    51121 gcaaaggaaa aagtagaact cagggtcgca gagcgaaaat ggttgtcttc actccagaga
    51181 atatcgcgcg tccagcggct gccatctttt attgccagcg cgttacgcag agggtcctgc
    51241 ccggaatttg tcacttccag cagattatta ttaagtttga cggtttttaa aagacgtcca
    51301 ttgtgggtaa ccagcatggc gttatcctgg gataaccatt tgctttgttc ttgttctata
    51361 tatcccagta ccacaaagat tcgttgccct tcattaaggc gtaaatacat agtggaataa
    51421 ggcaacgcct ggatctgctg atccgctacg gtgatatcct gattatcgaa aaggctggcg
    51481 cgaaatgtat cgaccatact ttgctggctg tgcgtacatc ccgcaagaaa tagtgcgaaa
    51541 atcgataaaa taagagggcg cacctttaat ccttgaaagg ttaattaaaa aaatgcaaat
    51601 ctctaaagcc cggtacgaat atcgcaaccg ggtatcattt aactttaatt tcgacgcgtt
    51661 aaagcagtat tagcgtgtag tggacgtggt ggttgaggtg ttatgagaac cgccatcgcc
    51721 actgtcattt gctgcaacaa ctgccaacgt cgccacacca acagccgttg ccacaccgac
    51781 aactgtcgcc gtgccgacag cgccagcagc cacgccacca gtggtagtag cagtcgtggt
    51841 tgcgccggta gtggtcgttg tggtgccagt agtacccgtc gttgcctcgg tcccgtttgt
    51901 tgcttcaggg gcagcaaacg cagctggcgt cgtcaacatg aaggccataa ggattgcaga
    51961 aagcttgtgt ttcataactt ttcctttatt catcgcatgg acagtacgta cgcttgagat
    52021 caggattgtc tactttgagt ataacatcct tgtgaaatct ccctttcctc ataccgcttg
    52081 cactatatac tgcgggtagg aaaggcgcgc agagggaaat ataagattgt taactatccc
    52141 cctctggaaa tacgacttgt gagcataaga taaatcttag caaaatacaa attaaagata
    52201 aggaaattgt tacgaaagct attaattatt gttggtaata tagtttcaag tggtactata
    52261 actggctgtt aaatggcatt tataactatt aggtgcagag atattcgctt aaatggatta
    52321 gtttctttgt ggaaaagtaa ctgatgttat aatgtatgat gttgactatt gcactattcc
    52381 aataaaacca gtcagcttta aacaagcagc gtcatattaa gagagataaa catttgccgc
    52441 tgttggtcct cgcaggccat ttacgcggca aaattccaca cgtaatcctg gtataagcac
    52501 ttctgcgtcg cggggagtga atgcggaaat atggacctga acttctttac gaccgtcgga
    52561 ggggataatg aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt
    52621 acgggacaaa caaattcctt actgaaaata ctgcgctgca ctatacgggg ttaataaaat
    52681 aaagccagcg atatttaaga ccgccggacg gctaaaataa aatttgctta atctcaatta
    52741 tcatgcgtta atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa
    52801 gcagttctca ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagta
    52861 gtttctgtat taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct
    52921 aataaaatga ctggtttagt aaaatggttt aacgcagata aaggttttgg ctttatcact
    52981 cctgatgatg gcagcaaaga cgttttcgtc catttcaccg ccatccagag caatgaattc
    53041 cgcacgctga acgaaaatca gaaagttgaa ttttctattg agcaggggca acgtggcccc
    53101 gcggcagcga acgttgttac gctctaaggt tgccattatt actcaacatc tccatttccg
    53161 ctgtccatgt tgtcatggtt cacagtaccg cacatcggca ttcgatgtga cggagcgaaa
    53221 ccctttgggc gctaagtgta ttttttgtaa atcgacgatg atcacctttg ataacgtcgc
    53281 gctgcaaata tgcactgacc atgcgccgct ggatttcaca aaataatatc aggctccctc
    53341 gtggagcctt ttttatatct gccttatttt tcttcaacgc tgtatgtata gtaagcgata
    53401 acctgttgat tattgaatct ttcggggaga tggcttataa catttcttac ctgaccaggg
    53461 taccgggaac caacacctta ctggcgtgtt gctgtctttt aagaccagaa gaggttaaca
    53521 gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt tatcgcgcaa
    53581 aaaatcgccg agctgaacaa gaatacaggg aaagaagtct ctgaaattcg cttcaccgca
    53641 cgagaaaaaa tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat gtgattttgt
    53701 gaacatcacc ccgtgcgagg tgatgttccg cttgttgcta atttagtgac caatcattgg
    53761 cgcttgtgga attaagcgtc ggtacaattc ctccggcacc gggctttgcc atactcccgc
    53821 atacattgcg taaccaatca ccgcgaacat aatccccaga accagtagcg tcattaacca
    53881 gccagacaac gcaaaagctt ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt
    53941 tgaggctacc ggacatgact ctacgcaagt catacagccg gtacattcca ctgttcgtac
    54001 ctgaattaat ttatcgaccg ggatccgtga tgggcaattt tttgcgcatt tgccacagcc
    54061 gatacaactt tcggcattgc gacgaatctt aaacggcgat aatagcgaaa ccacgcccat
    54121 cagcgcgcca tatgggcaaa gataacgaca ccaggcatgg cgaataaaca ggctggcaat
    54181 cagcaaaacg gtcacgctga ttaatgtcgc ggtccccata tgacgaaaga aatcgagcat
    54241 ttttacgtcc atcaccacgc tgtagggcga caacataaaa tagtgaatcg cctgagcggg
    54301 catcaataac gcgatataga gaaaaaaact caacagcaaa tacttcacgc cgcgcagagg
    54361 aatatccagc cagcggggaa ggacacattg ccgaccaaac agtttgttac cgagatcgcc
    54421 gattaattca gaaagcgtac caaccgggca taaccatgag caaaaggcct ttttgagtaa
    54481 tagactgatg acgataaaag cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa
    54541 ctgacctgtt gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc
    54601 gatacctccc ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca
    54661 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
    54721 acgccaggtc gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg
    54781 ccgccgctgc cagcgggttc tttttttctc tgccatgatt ttgccagtcc gttaagttgc
    54841 ataccaaatg ccactattct agttgttctt aactggctga tattgattca aatcgcgttc
    54901 aggtctttct tattcaacca tgcttccaga tcggcaacac tgcgtgtaat ttcttcgtgt
    54961 ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc
    55021 attcccagac tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta
    55081 tcctgactgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat
    55141 aataccagcc attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc
    55201 gatacatcca gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga
    55261 ccgagcactt cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc
    55321 tggcgcagcg tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat
    55381 tgctgtgcca gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc
    55441 agtgcggcag caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca
    55501 acagcaacaa cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc
    55561 gggttatctt caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt
    55621 tttggcacgg gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc
    55681 tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata
    55741 gtcagcccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt
    55801 tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc
    55861 tcgccatcag tgcgactacg caggataatg tacccttcgt cagtaaaacg cagggcgttg
    55921 ctcaacaggt tggttataac ctgacgaata cgtcgtggat cgcccattaa cgcgcacggc
    55981 atatcatcgg caattgccgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt
    56041 aattgcaggg tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca
    56101 ttcttgccac ctgcttcgat agcggaataa tcgagaatat cgttgaggat ggtcagcaac
    56161 gattcaccag agtcagtaat tgcccgcaaa tcatcacgct gggcgttaag tgcggggtta
    56221 tctgccagca gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc
    56281 atcgccgcca gaaacgccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt
    56341 cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc
    56401 tgttcgcggt ggcgattcag tgcatgaaca ttgctgcgaa acgcatccat cagccgcccg
    56461 atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc
    56521 agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc
    56581 acgcgccaga ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg
    56641 tattgcccgc gtgcactcgc tttttccaga tgcgccagtc cgtgctgatt acgcagctca
    56701 atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt
    56761 tgtgcgagag tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa
    56821 tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct
    56881 tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc
    56941 gttggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc
    57001 agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc
    57061 cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg
    57121 gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg
    57181 atctcatcgg cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgcagttgc
    57241 aaacgctgcc ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt
    57301 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
    57361 ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
    57421 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
    57481 atattcatgg taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc
    57541 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
    57601 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt
    57661 gagtcgcaaa gcggaatgca tctagcataa agccttatta ttgatgaggc tatcatgcgc
    57721 gtactgctat ttttacttct ttcccttttc atgttgccgg cattttcggc tgataacctg
    57781 ttgcgctggc atgatgcgca gcatttcacg gtgcaagcct ctacgccgct taaagccaaa
    57841 cgcgcatgga aactgtgcgc gctttatccc agcctgaaag attcatattg gttatcgttg
    57901 aactatggta tgcaggaggc tgctcgccgc tacggtgtgg atttaaaagt gctggaggca
    57961 ggcggctaca gccagttggc tacccagcaa gcacaaatcg accagtgtaa acagtggggc
    58021 gcagaggcca ttttgctcgg tagtagcacg acctcatttc ccgacctgca aaagcaggta
    58081 gcaagtctgc cggtgatcga actggtaaat gctattgatg ctccccaggt gaaaagccgc
    58141 gttggtgtgc cctggtttca gatgggctat caaccggggc gatatctggt gcaatgggcg
    58201 cacggtaaac cactgaatgt gctgttgatg cccggacccg ataacgccgg gggcagtaag
    58261 gagatggtcg agggttttcg cgcagccatt gccggaagcc cggtgcgtat tgttgatatt
    58321 gcgcttggtg ataacgatat tgaaatccag cgtaacctgt tgcaggagat gctggaacgc
    58381 catccagaaa tcgacgtcgt tgccggaacg gccattgcgg cagaggcggc aatgggggaa
    58441 gggcgtaacc tgaaaacgcc gcttaccgtg gtgtcgtttt atctttcaca tcaggtgtat
    58501 cgcgggctga agcggggaag agtgattatg gctgccagcg atcaaatggt ctggcagggg
    58561 gaactggcgg ttgagcaggc catcaggcaa ttacaggggc aatcggtttc tgataatgtc
    58621 agcccaccga ttttagttct gacgccgaaa aatgccgacc gtgaacatat tcgccgctcg
    58681 ctgtcaccag ggggatttcg tccggtctat ttttatcagc acacatcagc ggctaagaaa
    58741 taaccttcac catgttgcgt caccagtaaa tccgcgctga gtttatgacg taaacgacga
    58801 attaacacat cgacggtgcg caggtcaggg ttttccaccc gacgcgcaga aagcatacgt
    58861 agcagacgtt cacggctgag aatttcgccc ggattcgtca caaatgccac caacatttca
    58921 tactctgcgc gggtcagttt aatcggctcg ccatcccgct ccagcgtatg gcgcgacaca
    58981 ttcaggcaat aaccggcaaa gcgatagcag ttgtcctgag tgtgcggttg agcttgtcgc
    59041 gcgaggtcga ttcgccagag cagatttttc acccgtacta ccagttcgcg cagttccagc
    59101 ggtttggtga cgtaatcgtc tgcgcccatt tccagcccaa caatacggtc aatccgatcg
    59161 ctgcgtccgg taaccagaat aatccccacc gttgagcgtt ctcgcagggc gcgggttaac
    59221 atcaggccat tttcatcggg taagttgata tccagcagaa ttaaatctac cgactgattc
    59281 tgcataattt cccgtagccc ggcaccgctc gccgtaacgg aaacggtata cccctcctga
    59341 gtgaagtagg attgtaatcg cgcctgggta accggctcat cttcaacaat aacaatgtga
    59401 tgtggcatca gagggtttta ctcattctgt tcatatctgt tcatattctg ccgtaagccg
    59461 ttcatcctga ccagtgccgc tgttcatatt tgctcattaa gatcgcttca ctaaaccata
    59521 attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc agtgctcgtt
    59581 ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg attgtattgc
    59641 cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc cacagtatgc
    59701 aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc ggcgtgcgag
    59761 ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag cgcaaactgg
    59821 aagcgagcaa tgatatctac cagaccttta ttgctcactc cattgataca cctgaaaaat
    59881 tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa gaaaacaact
    59941 cggcaacctg ccgctcctgc cataactacg atgcgatgga tcatgcgaag cagcatcctg
    60001 aagcagcacg tcagatgaag gtggcagcga aagataatca atcctgcatc gactgtcata
    60061 aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag ttcgatgagc
    60121 tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt aagccaattt
    60181 atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg gaagtgaaag
    60241 tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg gaaagcgccg
    60301 gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc tcgattcgtg
    60361 gtgatgtgca gcagcaggta aaaacgctgg agaaaaccac cgttgccgac accaataccg
    60421 agtggagcaa gttgcaggcc actgcgtgga tgaagaaagg cgacatggtg aacgatatca
    60481 aaccgatctg ggcttatgcg gattcgttgt acaacggcac ctgtaaccag tgccacggcg
    60541 caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac ggcatgattg
    60601 gctttaccag tctcgataaa cgtgaagaac gcaccttgtt gaaatatctg caaatgaatg
    60661 cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa tgaacaataa
    60721 cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct taaccgtcgc
    60781 cgggatgctg gggccgtcat tgttaacgcc gcgacgtgcg actgcggcgc aagcggcgac
    60841 tgacgctgtc atctcgaaag agggcattct taccgggtcg cactgggggg ctatccgcgc
    60901 gacggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata aatatccgtc
    60961 gaaaatgatt gccggattgc cggatcacgt acacaacgcg gcgcgtattc gttatccgat
    61021 ggtacgcgtg gactggctgc gtaagcgcca tctcagcgat acctcccagc gcggtgataa
    61081 ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag aactggaacg
    61141 cgtgcagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc aatcgacggg
    61201 gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg gtaatagcgt
    61261 tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc gcgtagtcgg
    61321 ttcgatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga acagcaaaac
    61381 cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt ggtgcccgga
    61441 tcacgatgtt tatgaatatt acgcgcagct aaaagcgaaa gtcgccgccg gtgaaattga
    61501 ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc gcgagcatgt
    61561 gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc tggcacatac
    61621 gctgtacagt gaaaacctgt acgacaaaaa cttccttgct aactactgtg tgggttttga
    61681 gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg ccgcatgggc
    61741 tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc agatggcggc
    61801 gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg gtgaacagtg
    61861 ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc caggtggtgg
    61921 ttttggtttt ggctggcact acaacggcgc aggcacgccg gggcgtaaag gcgttattct
    61981 gagtggtttc tccggctcta cgtcgattcc gcctgttcac gacaacagtg actacaaagg
    62041 ctacagcagc actattccga ttgcccgttt tatcgatgcg atcctcgaac cggggaaagt
    62101 gatcaactgg aacggtaaat cggtaaaact gccgccgctg aaaatgtgta tttttgccgg
    62161 aactaaccca ttccatcgcc atcagcagat caaccgcatt attgaaggct tgcgcaagct
    62221 ggaaacggtt atcgccatag ataaccagtg gacctcaacc tgccgctttg ccgatatcgt
    62281 actgcctgcg accacgcagt ttgagcgtaa cgatctcgac cagtacggca atcactccaa
    62341 ccgtggcatt atcgccatga aacaggtggt gccgccgcag ttcgaggcgc gcaacgactt
    62401 cgatattttc cgcgagctgt gccgtcgctt taatcgcgaa gaagccttta ccgaagggct
    62461 ggacgaaatg ggctggctga aacgcatctg gcaggaaggt gtacagcaag gcaaaggacg
    62521 cggcgttcat ctgccagcgt ttgatgactt ctggaataac aaagagtacg tcgagtttga
    62581 ccatccgcag atgtttgttc gccaccaggc attccgcgaa gatccggatc tcgaaccgct
    62641 gggcacgccg agtggcctga ttgagatcta ctcgaaaact atcgccgata tgaactacga
    62701 cgattgtcag gggcatccga tgtggtttga gaaaatcgaa cgctcccacg gtgggcctgg
    62761 ctcgcaaaag tatccgttgc atctgcaatc tgtgcatccg gatttccgac ttcactcgca
    62821 gttatgtgag tcggaaacgc tgcgtcagca atatacggta gcgggtaaag agccagtatt
    62881 cattaacccg caggatgcca gcgcgcgcgg tattcgtaac ggtgatgtgg tacgcgtctt
    62941 taacgctcgc ggtcaggtgt tggcaggggc agtggtttct gaccgctatg cacccggcgt
    63001 ggcacgaatt cacgaagggg catggtacga tccagataaa ggcggcgagc ctggtgcgct
    63061 gtgcaaatac ggtaacccca acgtgttgac catcgacatc ggtacatcgc agctggcgca
    63121 ggcgaccagt gcgcacacta cgctggtgga aattgagaag tacaacggaa cagtggagca
    63181 ggtgacggcg tttaacggcc ccgtggagat ggtggcgcag tgcgaatatg ttcccgcgtc
    63241 gcaggtgaaa tcatgaccac gctgacagca caacagattg cctgtgttta cgcctggcta
    63301 gcgcagttgt tctcccgtga gctggacgat gaacaactga cgcaaatcgc cagtgcgcag
    63361 atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc tcactgcggc ggtgaacgag
    63421 ctggaaaacc gtattgccac gctgacagta cgtgacgatg cccgtctgga actggccgcg
    63481 gacttttgcg gcctgtttct gatgaccgac aaacaagcgg cgctgccgta tgcatcggcc
    63541 tacaaacagg acgagcaaga gattaaacgc ttgttagttg aggcagggat ggaaaccagc
    63601 ggcaatttca acgaaccggc agatcatctg gcgatctatc tcgaattgct cagccatctg
    63661 catttttcgc tgggagaggg gaccgttcct gcgcgaagaa tcgacagttt gcggcaaaaa
    63721 acactgacgg cgctgtggca atggttacca gagtttgttg cgcgttgtcg tcagtatgac
    63781 agctttggtt tttacgcggc actaagccag ttattgctgg tgttagtgga gtgcgaccac
    63841 caaaacagat aacgtcgttt gtgcgcctga aaagacgcgt ttagcgtcgc atcaggcatt
    63901 atggcgcagt tgccggatgc ggcgtgaacg tcttatccgg cccacaggaa ctgtaatctt
    63961 tgtagaccgg ttaagatgcg tcatcgcatc cggcaaacac acatcacgga tgagctacaa
    64021 accgggaaag ccgctggcgc agcaggcggt tttcctgctt caggtgcgca atatcatcca
    64081 ttaacgtcag cgccaccgcg atccccggcc agtccagagc cagttcatga cgcaggcgta
    64141 ccgcgcgttg caccacaatg gcggcatggt cgtcaaatac ccaggttgtt tcctggatct
    64201 cacgcggttc aaccaccccc aaaccgacaa tttcattcaa ctcctcttca gagatgccgg
    64261 tatgcaggca aaattcggta atagtaaaag tcaccgtaac attagccatt atgctttccc
    64321 ccaatcttta cgtggatcaa aagacgactg ggcgtctgcc agttgctgcc acagcgcggc
    64381 agtgttttca tccggtttcg gcggcatcac gattttcagt accgcataca gatcgccggt
    64441 ctgttttttg ctcaccagac ctttgccttt aacgcgcaat cgttgcccgg cctggctgcc
    64501 tggcgggata gtcagcaaaa tgctttcttt cagtgttgga acggtgactt tagcacccag
    64561 cgccgcttcc cacgggctaa ccggcaccac aatttccaga tcctggccga caatatcaaa
    64621 cagcggatgt ggcgcaatat gaatcaccag ccacaaatcg ccatttggac cgccgttttc
    64681 gcccggcgtc ccctggcctt tcagacggat gcgttgacca ttgccgacgc ccgccgggat
    64741 cttcacattc agcgttttcg gaatttcctg ttcgatcatg ccaaaggcgt tataaaccgg
    64801 caggttatag ctgatggtac gcttatgctc agtaagcgtt tcttcgagga ataccgccac
    64861 ttcgatttca atatcgtggc cgcgtgtggc ggggcgttga cggctctggc gggcatgctg
    64921 accgaaaatt gacgagaaga tatcgtcaaa atcttcggcg ttaaaactct gaccgtcgcc
    64981 atggtggaac tgacggttaa attgcggatc gttgcgatgt tgccacatct gatcatactc
    65041 agcgcgacgt tgttcatcac ttaacacttc ccaggcttca gcgacctctt tgaagcgggc
    65101 ttcggcatcc ggttctttgc tgacatcagg atggtatttg cgggcaagtc gacgataggc
    65161 ggtcttgatt gtcttgagat cgtccgtcgg tttcacgccc atgatggcgt aataatcctt
    65221 taattccata gcgttatctc gcgtaaatca acacaaattg aaggaacccc tgtaaggtaa
    65281 ctcctataag tgtagggtaa tcctcaaaat ttcatatgcc aacacagaat atgttattga
    65341 aatcatcgcg gagaggaggt cgccatcaag atgggttgct gaacatattt taaacaggtg
    65401 aaaaagggtg agcgattttt gatagttgaa ccaggcactt taagtttaac tagggcgtca
    65461 ttatttatta aattttatag acgctatata tgggtagtaa tatacatgga attagttgca
    65521 ctgcaaataa ttatttgaaa caggcctgga acgatataaa aaatgagtac gaaaaaaatc
    65581 aaacatattc aatcacgctt tttgaaaaca cactggtgtg ttttatgcgg ttatacaatg
    65641 aactcagacg taaagtaaat gaagaggata ctccatgtct ggaatgtgaa tcactagaaa
    65701 aagaatttga ggaaatgcag aatgataatg atctatcatt atttatgaga acattgcgta
    65761 ctaatgatac acaaatttat tcaggggttt caggaggtat tacatatact atacaatatg
    65821 ttcgagatat tgatattgtt agagtgtcct tgccgggcag agcttcagag tctatcacag
    65881 attttaaagg ttattattgg tataacttta tggagtatat tgaaaacatt aatgcgtgtg
    65941 atgatgtttt ttctgagtat tgttttgatg atgaaaatat aagtgtccag ccagagcgga
    66001 taaatacgcc gggaatatct gatttggatt ctgacattga tttgtctggt atatctttta
    66061 ttcagcgtga aactaaccag gcattaggat taaaatatgc tcctgtagat ggcgatggat
    66121 attgtctgtt aagagctata ctggttttaa aacaacatga ttattcatgg gcgctggtca
    66181 gttataagat gcaaaaggaa gtttacaacg aattcattaa aatggttgat aaaaaaacga
    66241 tcgaggctct tgttgatacg gcattctata atctcaggga agatgtaaag acgttatttg
    66301 gcgttgatct acaatctgac aaccaaattc aggggcagag tagtcttatg tcatggagct
    66361 ttctgttttt taaaaaacaa ttcattgata gttgcttgaa taacgaaaaa tgtatcctgc
    66421 atttacccga gtttatattt aatgataaca agaacttgct tgctttagat accgacacgt
    66481 cggataggat taaagcggtg aagaattttc ttgctgttct ttcagatagc atttgctcat
    66541 tatttattgt taatagtaat gtggcatcaa tctccttggg gaatgaatcc ttttcaacag
    66601 atgaagatct tgagtatggt tatttaatga acactggcaa tcattatgac gtttacctcc
    66661 ctcctgaact ttttgctcag gcttacaagt taaacaataa ggaaatgaat gcgcaactcg
    66721 actatttaaa tcgttatgca atttaatggc aaaggcatat gctaaaaacc attgttatta
    66781 gtctcacact tttttattgg taaatattgt ctctgtattg gtaacgccgc agatattctg
    66841 tttagccaca ggtgcaatta tcagcggcgt acgcgaggca ggggctaatc aggcatagtt
    66901 tgcgtcaaac cttgcctgtt tttgaagatg tatatagaaa aacaggcgtt caacaagcca
    66961 ttttgcgaac ctgttcccgg aaaaaagtca tatttctgtc acactcttta gtgattgata
    67021 acaaaagagg tgccaggaat gaacaaaacg ctaatcgccg cagctgtggc agggatagtt
    67081 ttactcgctt caaacgctca ggcacaaacc gtaccggaag gctatcagct acagcaagtg
    67141 ctcatgatga gccgccataa cttacgtgcg ccgctggcga acaatggcag tgtgctggag
    67201 cagtcgacgc cgaataaatg gccagaatgg gacgtccccg gtgggcaact caccaccaaa
    67261 ggtggcgtgc tcgaagtgta tatgggccat tacatgcgtg aatggctggc agagcagggg
    67321 atggtgaaat cgggggaatg cccgccgccg tacaccgttt atgcctatgc caatagtctg
    67381 caacgtaccg ttgcgaccgc acagttcttt attaccggcg cattcccggg gtgtgatatt
    67441 cctgtgcatc accaggaaaa aatgggcact atggacccaa cctttaaccc ggtgatcacc
    67501 gatgattccg ccgcattcag tgaacaggcg gtggcggcaa tggagaaaga gctcagcaaa
    67561 ctccagctta ccgacagcta ccagctactg gaaaaaatcg ttaactataa agattcccct
    67621 gcctgtaaag agaaacaaca gtgttcgctg gtggatggca aaaatacctt tagcgccaag
    67681 tatcaacaag aaccaggtgt ttccgggccg ctgaaagtcg gcaactcgct ggtagatgcg
    67741 tttactttgc aatattacga aggttttccg atggatcagg tggcctgggg agaaatcaaa
    67801 tctgaccagc agtggaaggt gttgtcgaag cttaaaaacg gctaccagga cagcctgttt
    67861 acctcaccgg aagtggcgcg caatgttgcg aaaccgctgg tcagttatat cgacaaagct
    67921 ctggtcaccg atcgcaccag cgcaccgaaa attacagtgt tggttgggca cgactccaac
    67981 attgcctctc tgttaacggc gctggatttc aaaccgtatc agttgcatga ccagaacgaa
    68041 cgcacgccga ttggcggcaa aatcgttttc cagcgttggc atgacagcaa agccaatcgc
    68101 gatttgatga aaattgaata tgtgtatcag agtgcggaac agttacgtaa tgccgatgcg
    68161 ttaaccctgc aggcacctgc gcagcgtgtg acgctggaat taagcggttg cccgatagac
    68221 gctgatggtt tctgcccgat ggataagttt gatagcgtgt tgaatgaagc ggtgaaataa
    68281 cagaaaactc ccccgcgaga agcgggggag tcgctggtta aacgttttta cgttcgatgg
    68341 tctgttcgcc ccaaaaaagc gaatctttat cggtcttagc aaaggctttg actaacactt
    68401 catcactacc ttcttcccaa atcttttccg ccattttttc gtcgtacccg gcgacttcga
    68461 aaatggcctc ggctatttcc ggcgacgtat tgcgcagaga tgcccattca ccgacgtgat
    68521 gagctttcgc ttcttgagtt ggcatgcgta tcctcctgtt gaagattagc cgttaagttt
    68581 aactgccaga cctgcgacat attccccttg ataacgagca atagacagtt cttcctggct
    68641 gggctggcgt gaaccgtcac cgcctgcgat ggtggttgcg ccgtacggcg taccgccgcg
    68701 aacctgtgaa acgtcaaata attcctgcgc tgcgtagcca atagggacaa ttaccatgcc
    68761 gtgatgcgca agggtcgtcc aggtggatgt gatggtttgt tcctgaccgc cgccagtacc
    68821 ggtggaacta aagacgctcg ccagttttcc gtatagtgcg ccggaagccc acaggccgcc
    68881 cgtctggtcg aggaaggtac gcatttgacc ggacatgttg ccaaagcggg taggtgtacc
    68941 aaaaataatg gcgtcgtaat cggccagttc ttgcggggtt gcaaccggtg cagtttgcgt
    69001 tttaccgcct gctttttcaa ataattgcgg cggcatggtt tccggtacac gcttaacgac
    69061 aacttcagcg ccatccactt tgcttgcacc ctcagcgact gcgcgtgcca tcgtttcaat
    69121 atgtccgtac atggaataat aaagcaccag aactttagcc atttctaacc actcctcgtg
    69181 ttatctctat tccgtagcga ttcgctacca cttatttaaa gataagacgt ccttttcaga
    69241 gtgcaaattt cacaaccact tatttgattt ataacaactt tcacaagcac gtaattttgt
    69301 cgcaaaatga cacattttta tctcatcgcg tttttttaat cataagagcg gcttatggat
    69361 aattattgga gatgatatct attctcgcta agaagctgtt gcaggatatt accaaacgcg
    69421 ggtctgcccg cgtcagttca ctaagcttag tcccacgtag cgaaaatatg gcagccgcca
    69481 tacgccgcgt taattctatg caatatgatg tctataccca gacggaggtc agtaatggca
    69541 aaccatcgag gcggttccgg caattttgca gaagaccgcg aaagagcatc agaagcaggt
    69601 aaaaaaggtg gacagcacag cgggggtaat ttcaagaatg acccgcagcg cgcatctgaa
    69661 gcaggtaaaa aaggtggtaa gagcagtcac ggcaaaagcg acaactagcc gggctaatca
    69721 atgacgaatg catttttgtc tgtagctcgt caaaaagcca tcaccgccgg ttacccggtg
    69781 gttgatactg atgacaaatg taagcttgcc tgatgcgcga tgcttatcag gcctaccaga
    69841 agattgcaat atattgaatt tgcactgttt tgtaggccgg ataaggcgtt tacgccgcat
    69901 ccggcatgaa caatgcgtac gttgtcaaca atctgcaccg ccggtaaccc cggcggtttt
    69961 ctgtttatgg ctcctgatga acaacttctg gcggtggaac gtcaaccaac tttctgctta
    70021 acaacgcatt gagtaaaatc gcgccaaagg ttgctgtacc aatccctccc aacgtaaaac
    70081 cgcccagcgt gagagcaaaa tcacccgcgc ccagcactaa ggttactgcg accataatca
    70141 aattaccgtt ctggcttaaa tcgacacggt tttgtaccca tatccttgcg cctgcgacgg
    70201 taatcagccc gaacacaaca attgatgcac caccaataac cgcggccgga atggtatgaa
    70261 tcagcgcacc aaatttcggt gaaaagccca acagcatggc gatgacggca gcagcaacaa
    70321 acaccagcgt cgagtagact ttggtcacgg ccatcacacc gatattttca gcataggtgg
    70381 tcacgccgct accgccgaca gagccggaaa gcatcgttgc cagaccatcg cctacgaatg
    70441 cccgccccat atacgggtcc atattgcgtc cggtcatccc ggcgactgcc ttgagatgac
    70501 ctaagttttc cgccaccaga atcaccgcca cgggcgcaat cagcatcatt gcctgaccat
    70561 taaaagcagg agtggaaaaa tgtggcagac cgaaccaggc agcatggctg acgagagtaa
    70621 aatcgacggc ttttcccagc cctaaaacgt tggtcatcac gccatacagc agacaggcga
    70681 caattaatcc tacgagaatc aataaccgct ggatcatgcc acgggtaaac accgccacca
    70741 gcccaataca cagcaccgtc attaccgcca tccagctatc aaaggccgaa gccgatacac
    70801 ttttcactgc gataggcgct aagttcaggc caatcgccat caccaccgca cccgtcacca
    70861 ccggcggcat cagtcgttca atccagcgcg taccgatttt catcaccacc aggccaatga
    70921 cggtataaac cagcccacag gcgataatcc cgcccagcgc aatgctgata ttcgggttaa
    70981 tgccctgacc gttaaagccc gtcgcggcga tcaccacgcc gacaaaagcc gcgctggagc
    71041 cgagataact ggggacgcgc ccgccggtaa taaagaaaaa cagtaacgtg ccgatccccg
    71101 acattaaaat ggaaagattg ggatccagcc ccatcagaat cggcattaac accgtcgcgc
    71161 caaacatcgc caccgcgtgt tgaacgccca ttactgccgt ctgagcaaac ggcaatcgtt
    71221 catccggcgc gaccacgccg ctctctgtag aggtcgattt taactgccag tgaggaaaac
    71281 cgaacattgc catcagctgt ctccttaagg aggttaacaa gcagggcgca tcagcgcgtg
    71341 ataactgcga tcgaaccaca ccagcccgta gggtgtggtg tgacgatgaa tcgcttcgat
    71401 ggcgcaaaac agaatgtcgt gggtgccgac gctcaccacc tggctgatac ggcagtcaaa
    71461 cgaaaccaga gcctcttcca gttgcgggca tccggtcacc cccgtctgcc agcgggcggc
    71521 ggcaaagcgg tgttccatgg gcgttttgcc gccaaaaagg tttgaaagcg gctcctgccc
    71581 ggcgctaagt gtatttacac acagcgttcg attttcattg aatgccggcc agacggacgc
    71641 cccacgattc aggcacacca gtaatgtggg cggcgtatcg gtcacactgc agacggcgct
    71701 ggcggtgaac ccggcgcgcc cggctggacc gtccgtggtg ataatattga ccgccgcgcc
    71761 catgcaggac atcgcatcgc gaaaagtttg ttgatcgaca atgttcatag tttgctcctt
    71821 acaacagccc gcaggcttct tcaaaggaca gacgtggcag gcgcgcataa agcttgctgc
    71881 tatcgccata gccgatatta atcagcagat tgctcttcag cgtgctgccc gtaaaaaagg
    71941 cgtcgtccac gtgttgacgg tcaaagcccg acatcgggcc ggtatccagt cccagcgccc
    72001 ggcaggcgac gatcagatag gccgcctgca tggaactgtt gcgaaacgct gtttcttcgg
    72061 caagttgtgg gctggaggta aaccaactgc gggcatcacc gtggggaaac agtagtggta
    72121 accgttcata aaattcactg tcccaggcga cgatagcggt gacgggcgcg gtcagggttt
    72181 tttgcagatt gccgctggaa agtgccgggc gcagacgttc ttttccttct gccgtgcggg
    72241 taaacacgat ccgtgccgga gaacagttag ctgatgtcgg cccccatttc atcagggcat
    72301 aaatctcccg taacgtctca tccctgacgg gtgtctcccg ccagccgttg tgagtgcggg
    72361 catcggtgaa cagggtgcta agcgcacctg ggctaacggc ttcgttcata gcaattcctt
    72421 acagggcggc ttcacggtga tgtaacaggc tggcaagccc gttgagtaac agagcattaa
    72481 acgtttcggg atcggtcacg ttgcaggcgt gtccgccata gggcatcacc attttctggc
    72541 tatcgggcag ggcggcatga agttcactgg aacatgctgt tggcaccagc agatcatcac
    72601 tggcgcagat gatttgcacc gggcagcgga tgcgatccgc atggtgacta aagtcagcgc
    72661 gtttgagggc gttaagtcga cgcagtaaat tatttttgcc ctgaaaatgc gccagtgcca
    72721 gcgcgtcttc tgcctccagg cgaggtgcgc gggccgccat ccagtcggcg ggatagagga
    72781 acaacggctg cgcttccacc catgcctgcg cgccgccgct atacagtaat cgttcgcgaa
    72841 cctgaaaaca gcggcgcgta tgggcgttta ttcgtagcca gccgttaacg ctgatcagca
    72901 cagttaccga cgcgggataa tccagcgcca gctgcattcc caccagcgca ccgagcgcat
    72961 ggccgaccac tgcgtaatgc tcaatccctg cggctaccag cgcctgatgc agttccgctg
    73021 ccatctgggc gatactgtaa tcttctgcca gcgtgtcggg attattgccg gtgccgcgct
    73081 ggtcgtaaca gactacctga tactcctgct ccagcaccgc cagttgcggt aaccagtaac
    73141 tgccgctacc cccaagaccc gaaatcaaca ccactacggg cgcatcagca taagggggag
    73201 gtgagagtga aagtttcatc gcggcctcac ttggcgatat gcgcaattgt ggcgatttcc
    73261 accagcgcgt caggttttac cagtccgcac tgaatgcaga atcgcgccgg tttatcaccc
    73321 ggaaaaaact cggcgtagat ttcgttaatc gcggcgtaat ttttccagtc ggtaataaag
    73381 atgctgttga aggtcacatc cgccatcgtg ccacccgccg tctcgatcac cttgcggata
    73441 gtttccagaa cgtggcgggt ttgcgccttt gggtcatcgg caaacagcac gttattatgt
    73501 tgatcaaaag ccagcgtacc ggagacatac accacgccat cagccagcgt gccgggaacg
    73561 aagggggcca gcggtgcgct gctgccagcg ggaataatta cggattttgg catcgttaaa
    73621 ctccttaagc gatatgagca aaggacgtgg gagaaagcgc gtcgcagaat gtttcgacgt
    73681 cgctgaccca gccaaaaaag gtttcgatat tgaacaacgc ggctttctgc gcaaatttcg
    73741 gccccgcctg gtgagttgcg tcttcaagca ccacgccgaa atactccaga aaaaagccgt
    73801 cgcgtagcgt cgattcgacg cagacgttgg tagcgatgcc ggtgaaaacc agatggcgta
    73861 ttccgcggct gcgcaaaatg ctgtccagcg gcgtattgaa gaaaccgctg tagcgcggct
    73921 tcggcagcac aatatcgcca ggctgcggca ccagttcatc caccagttga taatcccagg
    73981 agcctttcgc cagcaatttc ccctgcagct gcggctgctt acgcatggtt ttcagggcgt
    74041 tcgatttatg aaaattcggt gagccgggtc cgccagcctc gacatactgt tcatcccagc
    74101 cattttgaaa ccagatgatc agcatccctg ccgctcgcgc tgcggtcacg gcggtttgaa
    74161 tgttggcaat gaccgggcga gtggttgaga catcaaaccc ggcgagatct aagtagccgc
    74221 ctggcgtggc ataagcgttt tgcatatcca ccacgatcag cgcactttgc tgcggatcga
    74281 aggtaatggc ttccggtcga gcggttaagg tcgtcatcat gccacctcct gagtcagcgc
    74341 aggtagatgg gcgcggcatt gcatcagtgg ttgaatgcgc tcgccgaagg tttcgattcc
    74401 cgacagaaaa tcgtcgaagg ttaacagcac gccttcggca ccaggcacgc ttgcgacttc
    74461 atctaacatg cgcgcgacac tggcgtaaga accgactaac gtccccatat tgatgtttac
    74521 cgccgaagtg ggatcggcca tctgacgaac gttggtgtca gtaccggagc gggtatcttt
    74581 ctgactttgt tcggttagcc agcttaacgc ctcttcatcc gcgcccgctt tgtagtgttc
    74641 ccatttggcg cgagcggcat cgtcggtttc atcggcaatc accataaaca acacataaga
    74701 gccaacgtcg cgtccggttt gctctgcggc ctgtttcatc cgcgcagcgg tcggggcgaa
    74761 agccgtgggt gtatttacgc ctttgccgaa acagaagttg aaatcggcat accgggcgga
    74821 gaacgccatg ccagcgtcgc tttgcccggc gcagatcact ttcatgggga cactcggttg
    74881 cggactcacg cgacaatcat tcatggtgaa aaaatcgcct ttaaaatcgc ttttccccgt
    74941 gccccacagg tcgcgcagca cctgaacata ttcggtgaga tagtcgtaac gacgggagaa
    75001 atagtcatcg ccaggccaga tacccatctg ctcatactcg ggcttttgcc agccagtcac
    75061 gaggttgacg ccaaaacgcc cgccagagat ggagtcgatg gttgcggcca tacgggcgac
    75121 gattgccgga ggtaacgtta aggtggcagc agtggcgtaa atctgaatgc gcgaggtcac
    75181 ggccgccagc cccgccatca aggtgaacga ctcaaggttg tgatcccaga actcagtttt
    75241 gccgccaaag ccacgcagtt tgatcatcga cagggcgaaa tcgaaatggt agtgctccgc
    75301 tttttgcaca atggctttat tcagttcaaa ggtcggcatg tactgcggcg cgtgggtcga
    75361 aatgagccag ccgttgttgc caataggtac gaatacgcca attttcatca tcaacctctc
    75421 ttcgtctcgt aaagtgaaag tcagacgggg cgctgcatcc tgcatatcct tttcagccgc
    75481 gtattggctt gtttgcaaag cggatgccag tttttaaaaa gttaatgtta ttaatctgtt
    75541 aacattacgt tatctaaaat atctggtaaa aagtggacta aacggtcaaa acagttgcac
    75601 ataaaacatg catctgtgcg cgatgagagt gcagaaggtc gaggccgggc gggggttttg
    75661 ctatcctgtt gccaatctac aagaggggag agcgcatgac gcaaggcgca gtgaaaacaa
    75721 cgggtaaacg ttcgcgcgca gtaagcgcga agaaaaaagc gattcttagc gcagcactgg
    75781 acactttttc acaattcggt tttcacggca caaggctgga gcagatcgca gagttggcgg
    75841 gtgtttcaaa aaccaatctg ctgtattact ttccgtcaaa agaggcgctg tatattgccg
    75901 tgctgcggca gattctcgat atctggctgg caccgttaaa agcgtttcgt gaagatttcg
    75961 ccccgctggc ggcgatcaaa gagtacatcc gtctgaagct ggaagtctca cgcgattatc
    76021 cgcaggcttc gcgcctgttc tgtatggaga tgctggcagg cgcgccgctg ttaatggatg
    76081 aactgacggg cgatttgaag gcattaattg atgagaaatc ggcgctgatt gccggttggg
    76141 tcaaaagcgg caaactcgcg ccgattgatc cgcagcattt gatttttatg atttgggctt
    76201 ccactcaaca ttacgccgat ttcgcccctc aggtggaggc ggtgacaggc gcgacgttgc
    76261 gcgatgaggt atttttcaat caaacggttg aaaacgtgca gcggattatt attgagggga
    76321 ttcgaccacg ttaaagatgc cggaggaggt tgtaacatcc tccggctacc tgtttaacct
    76381 atagtcatta agctggcgtt accgccagcg gcagcggtat tcacactcag cgaacgctcg
    76441 atatacagcc gttccagaag gatattgctt tcgccacggg caaaaccctg caccgaaaca
    76501 attgtgccat cccgcgcggc aactgcttca cacaatgcgc gaagctgatc cgaatcaccg
    76561 tggaagatca ccgcatcaaa cggttgagcg gttatatttt ccgctttcgc cagttgaata
    76621 cgttcgctga ctgccgatgg caatgccttc actaactgac gatgcagcgc gtcatccggc
    76681 cacagtacct ggctgcccac cgccagcacg gcggcgagct gagtcagcgc atcctgctca
    76741 tcatcggcaa tacacaacac gcgctcacgc ggcagcagcg tccaggtgtt gcgttcaccc
    76801 gtcggccccg gcagcaatcg ttgtgttcct gcctgcgcca gctcgccata ttgcgtacat
    76861 aacgcctgca attctggacg atttgctgcc cattcccgca gtgcatttag cggctgagtc
    76921 aatgcggctt tcaactgcgc atcgaccgga tactttgcat cctgacgcgc gagcgtcact
    76981 gccagcgcac tttccgggcg attcgccagc agacggtaga gatagagcgg accgcctgct
    77041 ttcggcccgg taccggacaa cccttcgccg ccgaacggct gcacaccaac cactgcgccc
    77101 accatattac ggttaacata caggttacca acatgggccg agccagtgac ctgggcgatg
    77161 gtttcatcaa tgcgcgtatg gacgccaagc gtcagaccat aaccggaagc gttaatctgc
    77221 tcgatcagct ctggtagctg gttacggttg taacgcacca catgcagcac cggaccaaag
    77281 acctcttttt gcaattcggc aaagtcatcc agttcgatca gcgtcggggc gacaaaggtg
    77341 ccgctttgcc attcacgggc atcttcgctg ttttcccgca ccgcctggaa caccggacgg
    77401 cctttgctac gcatggtctg aatatggcgc tcaatattgg ctttcgcttc gctatcaatc
    77461 actggaccga tatcggtggt caggcgaccc ggattaccca tccggcattc ggccattgcg
    77521 ccgcgcagca ttttcaacgt gtggtcggca atctcatctt gcaggcacag cacgcgcagc
    77581 gccgaacaac gctgacccgc actgtcgaac gccgaggcca gtacatccac gacgacctgt
    77641 tcggtcagtg ctgaagaatc gacaatcatc gcgttcatgc cgccggtttc agcgatgagc
    77701 ggaatagggc gaccctgagc gtccaggcgg ctggcgatat tgcgctgcag taacgtagcg
    77761 acttcggttg aaccggtaaa catcaccccg cgcacgcgat catcacccgt cagttgcgcg
    77821 cccacggttt caccccgacc tggcagcaat tgcaccacgc ctggcggtac acccgcttcc
    77881 agcaaaatgg cgatcccttg cgcggcaatc agcggcgttt gttctgccgg ttttgccagc
    77941 acgctgttac ctgccgccag tgcggcggcg atctgcccgg tgaaaatagc cagcgggaag
    78001 ttccacggac tgatacacac cacaggccct aatggacggt gggtttcgtt agcgaaatca
    78061 tcccgcacct gtccggcgta gtagtggaga aaatcgaccg cttcgcgcac ttcggcaatg
    78121 gcgttactga aggtttttcc ggcctcacgc accagaatac caatcagttg ctgcatctgg
    78181 ctttccatca gcacggcagc gcggtgcaaa atcgctgcgc gttcagccgg aggcgtggca
    78241 aaccagattg gcgcgttatt aaccgcactt tccagcgcct gttctacttc acgcggcgtg
    78301 gcttcacgca catagcccac aatatctttc ggttccgcag ggttaataac gggcgacatc
    78361 tcacctgccg ctaccggttg ttccagcatt ggcaaggcct gccatttttg cagtgcacta
    78421 ttgagcaggg cagaggagag cgaggccagg cggtgttcgt tagcgagatc cagccctgcc
    78481 gagttgtcgc gcccgtgacc gtaaagatcg cgcggcaggg gaattttcgg atgcggtaat
    78541 ccagtttgcc cttcctgttg cgccagtttt tctacagcag tgaccggatc ggcgaccagt
    78601 tcatccagtg gcaaagaggt gtcggcaata cggttaacaa acgaggtgtt agcaccgttt
    78661 tccagcaggc gacgcaccag atacgccaac agcgtttcat gtgtgccaac cggagcataa
    78721 atacgacacg gacggttaag tttgccgtcg gcaactttcc cggtgacctg ctcatacagt
    78781 ggctcgccca taccatgcag gcactggaac tcgtactgac ccgggtagta gttctgcccc
    78841 gccagttgat aaatcgccgc cagcgtatgg gcgttgtgcg tcgcgaactg cgggtagatt
    78901 agattcggca ccgccagcag ctttttcgca caggcgagat aagaaacgtc ggtatacacc
    78961 ttgcgggtat aaaccggata accttcaagg ccgtccatct gcgcacgctt aatttcacta
    79021 tcccagtacg cgcctttcac caggcgaatc atcagacggc gacggctgcg ggtggcgaga
    79081 tcaatcaggt aatcgatcac caacgggcag cgtttttgat aagcctgaat aacaaaaccg
    79141 atgccgttcc agcctgccag ttccggctcg aaacagagtt tttccagcag atcgagggag
    79201 atctccaggc gatcggactc ttcggcgtca atgttgatac caatatcgta ctgacgcgcc
    79261 agcagggtga gtgatttcag acgcgggtaa agctcttcca ttacccggtc atactgggcg
    79321 cggctataac gcggatgcag cgccgacagt ttgattgaaa tgcccggccc ttcatagatg
    79381 ccacgaccgt tagacgcttt accgatggcg tgaatcgcct gctgatagga aaccatatac
    79441 gcctgtgcat ctgcggcggt cagcgcggct tcgcccagca tatcgtaaga gtaacggaaa
    79501 cctttctctt ccagcttgcg ggcattggct aacgcttccg cgatggtttc gccagtgacg
    79561 aactgctcac ccatcaggcg catcgccata tccacacctt tgcggatcag cggttcaccg
    79621 cttttaccga taatgcggtt cagcgagcgg gagaggctgg cttcgttatg ggtggaaacc
    79681 agtttgccag taaacagcag cccccaggtg gcggcattaa caaacagtga cgggctacga
    79741 ccaatgtgtg actgccagtt accgttgctg attttgtcgc gaattaacgc gtcgcgggtg
    79801 gctttgtcgg gaatacgcaa caacgcttcc gccagacaca tcagcgccac gccttcctgc
    79861 gatgacagcg aaaactcctg caataacccc tggaccatac ctgcgcgacc actggcattt
    79921 ttttgattac gcagtttatc ggccagctga tacgccagtt tgtgcgcctg ttcagcaact
    79981 ggctgcggca ggcgggcttg ttccagcagc atagaaaccg cttcggtttc cgggcggcga
    80041 taggccgcgg tgatcgcggc gcgggaaacc gactggggca atatttgctc ggcaaagtcg
    80101 aggaatggct ggtgtggttc ctctgccgga gtcggtgctt catcgctctc attggccgcg
    80161 ccagaaagca gcgcaggtag ctccggcaga gtatcgctgt tttccagttg ttcgagataa
    80221 gaaaaaatcg cctgcttaat taaccagtgt ggtgtgcgat cgatacgtgt cgcggcagac
    80281 ttaatacgct cacgcgtcgc gtcgtccagc ttaaccccca tggtggtggt tcccatgcca
    80341 ttactcctgt tgttcagaaa ggtgcaactt aacgttatcg tgaaatatcc atgatgttgc
    80401 aactttgtgc aaccatgtta aatgtgacat gcgtagcaag cttaaaaatg aatgaaatgt
    80461 taataaaaga aatcgatatg acagggatta aaaaaataac tcagactttt tctctgcggc
    80521 agttaacatt tttgaaaggt gcaaccgcaa aaaatgtgag agagtgcaac ctgatgaaaa
    80581 atagtgtcgc tgagcactaa aatttaatgt aaatggtgtg ttaaatcgat tgtgaataac
    80641 cagcgcttcc ggcaggatac ggtcgccctg gtaaaacata aactctgtta ccccgttccg
    80701 gtggcagata taacggcaag tttcgacatt gccgataata attttttgga gactttagat
    80761 ggctattagc acaccgatgt tggtgacatt ttgtgtctat atctttggca tgatattgat
    80821 tgggtttatc gcctggcgat caacgaaaaa ctttgacgac tatattctgg gcggtcgtag
    80881 tcttgggcca ttcgtgacgg cattatcggc gggtgcgtcg gatatgagcg gctggctgtt
    80941 aatggggttg ccgggcgctg tttttctttc cgggatttcc gaaagctgga tcgccattgg
    81001 cctgacatta ggcgcgtgga ttaactggaa gctggtggcc gggcggttgc gtgtgcatac
    81061 cgaatacaac aataacgcct taacactgcc ggattatttc accgggcgct ttgaagataa
    81121 aagccgcatt ttgcgcatta tctctgcgct ggttattttg ctgttcttca ccatttattg
    81181 cgcttcgggc attgtggcag gcgcgcgtct gtttgaaagt acctttggca tgagctacga
    81241 aacggctctg tgggcgggcg ctgcggcgac gatcctttac acctttattg gcggtttcct
    81301 cgcggtgagc tggactgaca ctgtacaggc cagcctgatg atttttgccc tgatcctgac
    81361 gccggttatc gtcattatca gtgtcggtgg ctttggtgac tcgctggaag tgatcaaaca
    81421 aaagagcatc gaaaacgttg atatgctcaa aggtctgaac tttgttgcca ttatctcact
    81481 gatgggttgg gggctgggtt acttcgggca gccgcacatt ctggcgcgtt ttatggcggc
    81541 ggattctcac cacagcattg tccatgcgcg tcgtattagt atgacctgga tgatcctctg
    81601 cctggcaggg gcggtggctg tcggcttctt tgggattgct tactttaacg atcatccggc
    81661 gttggctggt gcggtaaatc agaacgccga gcgtgtgttt atcgaactgg cgcaaattct
    81721 gtttaacccg tggattgccg ggattctgct gtcggcaatt ctggcggcgg taatgtcaac
    81781 cttaagttgc cagctgctgg tgtgctccag tgcgattacc gaagatttgt acaaagcgtt
    81841 tctgcgtaaa catgccagcc agaaagagct ggtgtgggta gggcgtgtga tggtgctggt
    81901 ggtggcgctg gtggcgattg cgctggcggc aaacccggaa aaccgcgtgc tgggcttagt
    81961 gagctacgcg tgggcaggct ttggcgcggc gtttggtcca gtggtgctgt tctcggtgat
    82021 gtggtcacgc atgacgcgta acggtgcgct ggcggggatg atcatcggtg cgctgacggt
    82081 tatcgtctgg aaacagttcg gctggctggg actgtacgaa attattccgg gctttatctt
    82141 cggcagtatt gggattgtag tgtttagttt gctgggtaaa gcgccgtcag cggcgatgca
    82201 aaaacgcttt gccgaggccg atgcgcacta tcattcggct ccgccgtcac ggttgcagga
    82261 aagctaaggg acttagcctg cggcggtttt gtttggcttc agcagcgggt tgcgctccct
    82321 taatgtgcct cgccatataa attgaatggt gcagggagcg cgcagggggc ggccaatcgc
    82381 cgccgccccc tgctgtcccg gccttcgggg aacgcttcag cgattttgac gccaccaaca
    82441 cccgagctgt tattatgttc cgggcaaaaa gttagatttg ataatcgcgg atggacgaaa
    82501 ttgcttgata cacccgctta tcagttttac atggaagctc tgatgcattg agtctggaca
    82561 gttttgtcgg ctggatacgg cgtttacgcg gcatccggca agaacacatg gttctttgca
    82621 aacaatccca tctttctacc ctggaataat cgtttatatc ccttggcatt acctctcttt
    82681 gtttacattc caacatcatt ttataaacat tccgcttgtg tttttctttg ccgtaatgat
    82741 aatcgctatc actgcgattt acttttcttt gcatagattg actcagaaaa acgtttaagg
    82801 gtgggtggca tgtttgttcc gtttctcatt atgttgcgcg aaggacttga agccgcgctg
    82861 attgtcagtt tgattgccag ctatcttaag cgtacccagc gaggccgatg gattggtgtg
    82921 atgtggattg gcgtgttgct tgccgctgcg ttgtgcctgg gcttgggtat cttcattaac
    82981 gaaaccaccg gcgaatttcc gcaaaaagaa caggaactgt ttgaaggtat cgtggcggtg
    83041 atcgccgtgg tgatccttac ctggatggtt ttctggatgc gcaaagtgtc gcgcaacgtc
    83101 aaagtgcaac tggaacaggc agtcgatagc gcattgcagc gtggaaatca tcatggctgg
    83161 gcgctggtga tgatggtctt ttttgccgtt gcaagggaag ggctggagtc ggtctttttc
    83221 ctgctggcgg catttcaaca agatgtcggg atctggccgc cgctgggtgc aatgctcggt
    83281 cttgctactg ccgtggtgct aggcttcctg ctctactggg gcggtattcg cctcaatctt
    83341 ggtgcatttt ttaaatggac cagcctgttt attctcttcg tcgccgcagg gctggcagct
    83401 ggtgccattc gcgcatttca tgaagccgga ttgtggaacc actttcagga aatcgccttc
    83461 gatatgagtg cggtgctctc aactcactcg ctgtttggca cgctgatgga agggattttt
    83521 ggctatcagg aagcgccgag cgtcagcgaa gtcgccgtct ggtttattta tctcatcccg
    83581 gcgctggtgg catttgctct gccaccacgc gcaggggcga cagcgtctcg ctccgcgtaa
    83641 caaatacgac gcaaactctt gcttagttac aacatacttt aaagggatag tctcgtcatg
    83701 accattaact tccgccgtaa cgcattgcag ttgagcgtgg ctgcgctgtt ttcttctgct
    83761 tttatggcta acgccgctga tgtgccgcag gtcaaagtga ccgtgacgga taagcagtgc
    83821 gaaccgatga ccattacggt taacgccggg aaaacacagt tcattattca gaaccacagc
    83881 cagaaggcgc tggagtggga gatcctcaaa ggcgtgatgg tggtggaaga gcgggaaaat
    83941 atcgcccctg gctttagcca gaaaatgacg gcgaatttac agcctggcga atacgatatg
    84001 acctgcggtc tgctgactaa cccgaaaggg aagttgatcg tcaaaggtga ggcaacggcg
    84061 gatgcggcgc aaagtgatgc gctgttaagt cttggtggtg caattactgc atataaagcg
    84121 tatgtcatgg cggaaaccac gcagctggtg accgacacca aagcctttac cgacgcgatt
    84181 aaagcaggcg atatcgaaaa agcgaaagca ctgtatgcac cgacgcgcca gcactatgag
    84241 cgtattgaac cgattgctga actgttctcc gatctggatg gcagcattga cgcccgtgaa
    84301 gatgattacg agcaaaaagc cgccgaccca aaattcactg gtttccaccg tctggaaaaa
    84361 gcattgtttg gcgacaacac caccaaaggg atggatcagt acgctgagca gctttatacc
    84421 gatgtggtcg atttgcaaaa acgcatcagt gaactggctt tcccaccttc aaaagtggtc
    84481 ggcggcgcag ccggactgat tgaggaagtg gcagccagca aaattagcgg tgaagaagat
    84541 cgctacagcc acaccgatct gtgggatttc caggctaacg ttgaaggctc gcagaaaatt
    84601 gtcgatttgc tgcgtccaca actgcaaaaa gccaacccgg aactgctggc aaaagtcgat
    84661 gccaacttta aaaaggtcga taccattctg gcgaaatacc gtactaaaga cgattttgaa
    84721 acctacgaca aattgaccga tgccgaccgg aatgcactga aaggaccgat tactgcgctg
    84781 gcggaagatc tggcgcaact tcgcggtgtg ctgggactgg attaagcgtt atgcagtata
    84841 aagatgaaaa cggcgtgaat gaaccgtcac gccgacgttt actgaaagtg ataggtgcac
    84901 tggcgctggc gggaagttgt ccggtcgctc atgcacaaaa aacgcaaagt gcgccgggta
    84961 cgctttcacc ggatgctcgc aatgagaaac agccgtttta tggtgagcat caggcaggga
    85021 tcctgacgcc acaacaggcc gcaatgatgc tggtggcgtt tgatgtgctt gccagcgata
    85081 aagccgatct tgagcggttg tttcgcttgt tgactcagcg ttttgctttt ctgactcagg
    85141 gcggagcagc accagaaacg ccaaatccgc gcctgccacc actcgattcc ggcattcttg
    85201 gcggctacat tgcgcccgat aatctcacca tcacgttatc ggtgggtcac tcattgtttg
    85261 atgagcgctt tggccttgcg ccacagatgc caaaaaagct gcagaagatg acgcgtttcc
    85321 ccaacgactc gctggatgcg gcgttatgtc atggtgatgt gttgctacag atttgcgcca
    85381 acacccagga cacggttatc catgcgctgc gcgatatcat caaacacacg ccggatttgc
    85441 tcagtgtgcg ctggaagcgg gaagggttta tttccgatca cgcggcgcgt agtaaaggca
    85501 aagagacgcc gattaatttg ctgggtttca aagacggcac tgccaatccc gatagccaga
    85561 atgataagtt gatgcaaaaa gtggtgtggg taacggcaga tcagcaggag cctgcgtggg
    85621 caatcggtgg cagctatcag gcagtacgct tgattcagtt tcgagtggaa ttttgggaca
    85681 gaacgccgct gaaagaacag cagacgattt ttggccgtga taagcaaacc ggtgcgccgc
    85741 tgggaatgca gcatgagcat gatgtgcctg attacgccag cgacccggaa gggaaggtga
    85801 tcgcgctgga cagccatatc cggctggcga atccccgcac ggcggagagt gagtccagcc
    85861 tgatgctgcg tcgtggctac agttattcac tgggcgtcac caactccggg caactggata
    85921 tggggttgct gtttgtctgc taccaacacg atctggaaaa aggcttcctg acagtacaaa
    85981 aaaggctcaa tggcgaagcg ctggaggaat acgttaaacc tatcggcggc ggttattttt
    86041 ttgcgctgcc gggggtgaag gacgcgaacg attatttcgg aagcgcgtta ttgcgggttt
    86101 aatgttttta ggcggataag gcatttgtgc gcagatgcct gatgcgacgc ttgcgcgtct
    86161 tatcatgcct acaatcagtg agggtttggt aggctggata aggcgttcac gccgcatccg
    86221 gcgatcgtgc actgatgcct gatgcaaatc ctgctgaaag cacacagctt ttttcatcac
    86281 tgtcatcact ctgtcatctt tccagtagaa actaatgtca ctgaaatggt gttttatagt
    86341 taaatataag taaatatatt gttgcaataa atgcgagatc tgttgtactt attaagtagc
    86401 agcggaagtt cccggcagtg atagtcagtc actatggaga tcgcggatgg taacgtcctg
    86461 tactggacat gttttagata atcaacgcgc caccactcgc ggagttttct cctccggtag
    86521 tcatctcgtc actttgcatt ttcaacctca tcctttcttt tcatgtgtta ccgacgccgt
    86581 aaacggtgcg cgtagccgtt tttcggcatt ttatccaaaa gcaaactatg gcttacaagg
    86641 aagccaaccc tctgatgttc gtgcgcataa tcgcgctgcc aacggcgcgt gtgatgaata
    86701 caaacaactc aaggtgctct ccatgggaag acaaaaagca gtgatcaaag ctcgtcgcga
    86761 ggcaaaacgt gtgctgagac gggattcgcg cagccataag cagcgtgaag aagaatcggt
    86821 cacctcgctt gtgcagatgg gcggcgtaga agccattggt atggcccgcg acagtcgcga
    86881 tacttcgccc atcctcgcgc gaaatgaagc gcaattgcac tatctgaagg ctattgagag
    86941 taagcagctg atattcgcca cgggcgaagc cgggtgcgga aaaacctgga tcagcgcagc
    87001 aaaagcggca gaggccctga tacataagga tgtcgacagg attatcgtca cccgtccagt
    87061 tctgcaagcc gatgaagatc ttggcttctt acctggagat atcgcagaaa agtttgctcc
    87121 ctattttcgc ccggtctacg acgtgctggt ccggcgctta ggggcttcct ttatgcagta
    87181 ctgcctgcga ccggaaatag ggaaggtgga aattgcgccg ttcgcctata tgcgtggacg
    87241 tacctttgaa aatgcagtcg tcattcttga cgaggcgcag aatgtgactg ccgcgcaaat
    87301 gaaaatgttt ttaacccgcc tcggggagaa cgtgacggtt atcgtcaacg gtgatatcac
    87361 gcaatgcgat ttgcctcgcg gcgtgtgctc cggattaagt gacgcgctgg aacgtttcga
    87421 agaagatgaa atggttggga tcgtccggtt cggtaaagag gactgcgtac gttcggcact
    87481 ttgccaacgt acgctgcatg cctacagtta agtgtgttat cggtgcagag cccgggcgaa
    87541 ccgggctttg ttttgggtgt ttatgcccgg actagcgctt tttctgaaac aaccattttt
    87601 atttgcccct ggctggtgaa gtgtacgctc atcctgtggc ttttttgtag ttgctgatag
    87661 agctcatcag gtattgctaa gctctctgca tattcttgcg gcgtatattg gtaggctgca
    87721 tgatgctgct gtttttgaaa acgcagctta ttgtacagcg cccagacaat taacacgacg
    87781 gcattcgcca ctgccagcaa aaaatagaac tgaagtcggc ttctggcctc gctttgccag
    87841 taataacccg tcagcagatc catggcgaat aagaatatga acaacgcaaa taatgtccac
    87901 aagatggttg tggcaacata atcaaccagt aaacgtactg gtgattgtcg ggtcgtaata
    87961 attaaattgt tcatattaac ctctcagaat cccgcgatcg ggacttaccc aacgggcgcg
    88021 ttgcttttta ggcatcaaca tgactcgtgt aaatgatacc aatgtcgttg ccaggctcag
    88081 catccagaaa ataaccggga accaaataat ccagaaaagc gatgaagtca gattatgctc
    88141 atagcgattc tcgatcatca ggctgacaat aaattgcagt aaacataacg tacacaataa
    88201 tattccggca gtatgtgtcg cagcgatatg tgtcaattca atatttaacg gtacaccggc
    88261 aagttggact gcgtaaataa tgaaaccgac caggcaggtg aaggcccata ttgtcgtcag
    88321 gcagtattca aaaaacagcg gccacattcg aaagttttct ttgcgccaca accttgtcat
    88381 atttttgagg aatacttctg caccgccctg agcccagcgc aggcgctgtt tccacagccc
    88441 ttttaacgtt tcaggcatta atatccagca cagtgcccgt ggctcgtaaa aaatcgtcca
    88501 ctgattcaac tgcagcttcc agctaatatc aatatcttcg gtgatcatat cgtcactcca
    88561 gtaacccact tctgccaggg cgctgcgacg aaatgcggca ataacaccgg aaacggtaaa
    88621 tacgtttcca tagatacgct gggttcgctt gatcaaacca ataattgagg aatactcgcc
    88681 aacctgaatt ttacccacca gggtagaacg tgttcgaata cgaggattac cggttacggc
    88741 acccacacgc gggttgtaca acatcggttc cacaatatat gccgccgcat cgcggtctaa
    88801 taacgcatcg ccatcaatgc acaccagata ttcacttttc gccgcggcag ctccggtttt
    88861 aagcgcaatg gctttcccct ggttttgcgc cagatgaatg acccgcaaat ggggaatttg
    88921 tgcagccatg cgatccagga tggcacgggt tttatctgtt gaaccgtcat ttacggcaat
    88981 aacttcaatg ttctcataac gctgtgctaa agcggcgtgt atggtttcct caacgttttt
    89041 ctcctcatta aaacagggaa tgataatgga gatagacgga ttatctttca actggggagc
    89101 tggtgcgttt tctccccacg gccagtggcg ttcacgatag acccagaaat agacgccgcc
    89161 aacaatccac ataatggaca taaaaaacgg ccagaagaaa acgaacctca tcatcagttc
    89221 accagagtga aagtacgcta cgcatagggg tatgcataac accagacata atataaaaaa
    89281 cgatacgatg cgattaatca tttttcggat accaggctgt tgaaaactca ggacgaataa
    89341 ggtctatttc aggttggtta tgcagaaaat tgtcgggata ataaccatag tttttcactc
    89401 cattcagttg taataggctc atccagtgag cgagttgttg cgaagaaata gcctgatgct
    89461 gaccattttt ctgccagttt tgtgcctgta attctaaaat agatttgtct ttagcctgag
    89521 ggatgttttt aatttgattg gtcaattgta ttaaccattg gtcagccgat ttttctgcga
    89581 caccttccag ataaggcata gccataatag cggtccagtc atagcttttt aggaaatcag
    89641 cataattctg tgcaaaccag gcttcacttt caggttgtat taccggaagt gcaaaaatat
    89701 ttcgtgcagt tttaatatgt ggaccgcgaa tggcttttac gcgcgcacta agttctaaag
    89761 tgaagtcagt taacgcacga cttttaaagc gggcccactg tttaaattgc tccgggtttt
    89821 gtcgaatttc gctcagactc ccgctaaagc ctgcttgctg ataagccgtg atagccggtg
    89881 cactggcatc ttcataatct gaaagcaaag catcatcgtg gaacaatatg ccatcaaaag
    89941 cagcatgtcc ggcaagatct tcatataaca tgccaacttg tgctctgact ctgtcatcga
    90001 aaggagagag acggtgatat tgttcaggat gaatttgtgc ttttttctcc cctgttggta
    90061 agtattttac tcgcgttaat gtgggatcta aatcccagct taataccggc atccacgcat
    90121 agatgtttac acctgagcgg gtacgtaatt gccaggcaac ccgactaaaa atatctgctt
    90181 tcattggtag caaacgattt ggaaaccaga cctctttgac cagcccatca ccatcgggat
    90241 cagcaaatgc ctgcaaatac acggttgata tttgcatatc tttcacccgc tgaattagca
    90301 catcaatatt gcgatccatt tgctggaggt tttcgtcata aacgtaatca agatcgatat
    90361 gcattatccg ttgtggtgat ttttcctgta cggtaataat ttgctgggca aactctttta
    90421 atgagggatt attggcgatt aatacccgcg gaatggaatc caattgcgac gcatttgcca
    90481 aacctgattc aagggtgaag aacatgtcat aaccgagttt ttttaattcc tctatcgcta
    90541 tgccattcgc ttcgccataa ggccaaacaa aaacgtgtgg atttacctca acctttgtac
    90601 gcaggtattc cgtcattttt acagcatcca gacgaattct ttcccggtat tctgctgcgg
    90661 tttcataccg tgcgtggtca gtaaaatatg cacgatttac atatacaggc aataagctgc
    90721 cggtggcatt agcctgaata ccgtagtgag aattccatgt atgagaagcg agctcaacga
    90781 gccgggaacg cgcaacttct cgcacttgtt gccacgtggc aaaatattct cgatcgacca
    90841 actcatcgcc aaattttact tgtttatccg ctggcgtatc gacccaactg ccgacggggg
    90901 cccatacagc aggccactgg aaggcctgaa gaattgggaa gacgcgggta taaaaactct
    90961 ggtagccgtc atcaaaagtc agcactacag ctttttccgg tagcggtttt cctcctcgat
    91021 gtgcttcacg aatttgagca atactgaccg gttgataacc gttctcgcgc agccaggcaa
    91081 attgttcacg cagtgctgat gtccgcactg acataaaacg ctggtcggca gcttcgtctt
    91141 caacgttatg ccatgaaatc gctacaaaac cattatgcgg ccacggttgc tcggcgagta
    91201 aagattcgcg atcctgtggc ggtataaatg atgttcttga ctggctaatg cacgcggtga
    91261 gcataattat actcaccagc atcaggagat atttatttcc attacgtaac atatttatcc
    91321 ttaaaatctg aatgtcatat cgaattcaac gtataagttg tgttctctgt caccgtcata
    91381 aggtcgtttt tcccagcgta gcgttgcgcc agcatcaatc acgtcattcc aactaatgcg
    91441 ttgcccgtag ccgagttggg tgacgacatc cgtgccataa tgtttttgcc aggaggcacc
    91501 aacacctgcg ctgaatattt gctcccagct attttcatag cttcgccata acaaatggct
    91561 tgcctcaaat gccggaacaa tatcgaacgt ttttataggg ttgtagtatg gggtatcgtg
    91621 ttctgtattt tgttcgtaat acagactggg taggaaatcg acaatcaaat atggtgaaga
    91681 ccagatgcgt tcctgaccct caagtgagac ttcatgacgc tggttactgt cggaaaaatc
    91741 agtgaaagcc caggagatac cgtacttacg ccgctcattt tgataccagc gaacataagc
    91801 ctgagcactg ttgcctgtaa caccattttt cattgcccgt aatggaacgc ggtgagagag
    91861 gcgttccagt tgcgaaccaa tacgccagtt atcattaaaa tcataccagc cagacaggcg
    91921 cgcgccgggt ttatgctcat gattgaaaac gcgttcagcg tactctgcct cgagccagat
    91981 attacgtgac cgccactcaa cacccgcaag ccagtcgcga acaatccctt ttccttcgct
    92041 aaattgtcca tcggcataac cgaatccagc aaaaccgcgc cagttatcct tcagcggtgg
    92101 tgaataaacg atggtggtta agtcgatatc atgtttacca ctatccgggc cttcggcatc
    92161 aattcctgtt gagccagcga tacgaagctc tgcaagatta tgtacatcaa ccgcacgttt
    92221 taatcgtaca acgccgggat cttgcggttc acgttcgaca acatcgtgcg ttaagacagc
    92281 tgcctgctgc cattcttgta acgttaatgc tgtccaggct tgttcaacct ccagattaat
    92341 attacgtggc tcgatcactt ctgctttttt taattcattt tctgctgcac gaggccaacc
    92401 gcgggcttgt aacacactcg cgtaatcaat gcgcagtccc tgatttcctg gtgcgttata
    92461 agcaagctct ctggctgtca tttcagcctg aggaagatca ttactatatt ttgctacggt
    92521 tgagagaaac gaatgcccct gtaaccaggt atcattcggg atgctcgtag gcgtgcccat
    92581 taaccgaagg aaaggcggcg aagtattaat ggtatgttgg gtgacagtta gcgcgcccgg
    92641 ataattttca ctctccaggt ggctgtaaaa gagatccgca agttcttcat cggataaatc
    92701 cggggcaatg gtctccttgt gataaaagag ctcggtcatt attgactgtg cttttttcgg
    92761 ctgatgatct ttgagataag ccgatgcaac ccaatattgc ccccagggcg gaataatttg
    92821 ccccgtcttt tttaatcgct gatagtgaga aataacgtct ttataacgat cgcgagttaa
    92881 taacgcgcca agatgatcaa cctgaatacg ctggtactgg gcagtgcggt ctgggttatc
    92941 gtgccacaga atttctaatg cagcgtattg ggcgagggcg cgatcggcaa tggcataacg
    93001 ttcactttca ctgcgcgtag gcataaacga cagtctgacc agttcggcat gaatatcagc
    93061 gcgaatatct ggcgttaaat tggcatcgtc aatcgcggca gcaagttgat tattacgtaa
    93121 tgcctgcacg tattctgtgg gatattgttg cgtagatgca ttttcaggta atgactctgt
    93181 catcgcccgt aattcatcct gatgacgccc cgccagttta tagatatagg cttctgcgag
    93241 taaattggct ttgtccggtg ctccagagtt aagctgctta agtttaacca gcgcagtatc
    93301 atagtgacca gcatctgcca gggttaaaat ttgtccccgt tgataatcct tattttgcgg
    93361 ctccagagag agcgcctttt gccacagtgt aagcgagttt tgccattgtt gcaggttacg
    93421 ataagcgacg gcgacagctg cataaccacg cgctggtaat tgctgatgac ggtagcggtt
    93481 gtaaacggta ataacctgtt tatcttgccc ggcccataag gcaatctgta accagtcagc
    93541 aatttggtta ttgctgagtg ctgattttag tgcaaaccat gacaaagctg gctgagtatt
    93601 acccttgcga gcttcaataa tcaatgcatc ataggcgtta ttaacagcac tcttcgccgc
    93661 gggactcgct gctaaaaatg cggcagtaag aagtttcaaa gcccatttgg ttttcgggca
    93721 cctttttctg ctacttgaat acatcctgta ttactccatg tattgccaaa atctctctct
    93781 gtatctaatt acaggtaact gaaaagaaag atatttttgc acctcataat ccgttattaa
    93841 acgcggaaga gagacgtgaa ttgttgatga tgagaagaag aaatgatgag cagagtgtcc
    93901 atataaaatc cttttctcgc ccgaaaatcc attccaatga tgaggatctt caggaatacg
    93961 gcataaatcc caa
//
LOCUS       DBBGLQ010000007        93762 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.007, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000007 DBBGLQ010000000
VERSION     DBBGLQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 93762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.007"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(11..556)
                     /gene="hemG"
                     /locus_tag="ACIYSB_000670"
     CDS             complement(11..556)
                     /gene="hemG"
                     /locus_tag="ACIYSB_000670"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGC5427783.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            complement(568..2019)
                     /gene="trkH"
                     /locus_tag="ACIYSB_000671"
     CDS             complement(568..2019)
                     /gene="trkH"
                     /locus_tag="ACIYSB_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGC5427784.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2058..2672)
                     /gene="yigZ"
                     /locus_tag="ACIYSB_000672"
     CDS             complement(2058..2672)
                     /gene="yigZ"
                     /locus_tag="ACIYSB_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGC5427785.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="ACIYSB_000673"
     CDS             complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="ACIYSB_000673"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGC5427786.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4193..6382
                     /gene="fadB"
                     /locus_tag="ACIYSB_000674"
     CDS             4193..6382
                     /gene="fadB"
                     /locus_tag="ACIYSB_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGC5427787.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6392..7555
                     /gene="fadA"
                     /locus_tag="ACIYSB_000675"
     CDS             6392..7555
                     /gene="fadA"
                     /locus_tag="ACIYSB_000675"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGC5427788.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGMVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7838..8539)
                     /gene="fre"
                     /locus_tag="ACIYSB_000676"
     CDS             complement(7838..8539)
                     /gene="fre"
                     /locus_tag="ACIYSB_000676"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGC5427789.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8585..10078)
                     /gene="ubiD"
                     /locus_tag="ACIYSB_000677"
     CDS             complement(8585..10078)
                     /gene="ubiD"
                     /locus_tag="ACIYSB_000677"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGC5427790.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10245..10733
                     /gene="rfaH"
                     /locus_tag="ACIYSB_000678"
     CDS             10245..10733
                     /gene="rfaH"
                     /locus_tag="ACIYSB_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGC5427791.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10730..11512)
                     /gene="tatD"
                     /locus_tag="ACIYSB_000679"
     CDS             complement(10730..11512)
                     /gene="tatD"
                     /locus_tag="ACIYSB_000679"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGC5427792.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVACAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11554..12330)
                     /gene="tatC"
                     /locus_tag="ACIYSB_000680"
     CDS             complement(11554..12330)
                     /gene="tatC"
                     /locus_tag="ACIYSB_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGC5427793.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12333..12848)
                     /gene="tatB"
                     /locus_tag="ACIYSB_000681"
     CDS             complement(12333..12848)
                     /gene="tatB"
                     /locus_tag="ACIYSB_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGC5427794.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12852..13121)
                     /gene="tatA"
                     /locus_tag="ACIYSB_000682"
     CDS             complement(12852..13121)
                     /gene="tatA"
                     /locus_tag="ACIYSB_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGC5427795.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13200..14840)
                     /gene="ubiB"
                     /locus_tag="ACIYSB_000683"
     CDS             complement(13200..14840)
                     /gene="ubiB"
                     /locus_tag="ACIYSB_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGC5427796.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14837..15442)
                     /gene="ubiJ"
                     /locus_tag="ACIYSB_000684"
     CDS             complement(14837..15442)
                     /gene="ubiJ"
                     /locus_tag="ACIYSB_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGC5427797.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15456..16211)
                     /gene="ubiE"
                     /locus_tag="ACIYSB_000685"
     CDS             complement(15456..16211)
                     /gene="ubiE"
                     /locus_tag="ACIYSB_000685"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGC5427798.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16306..17733)
                     /gene="rmuC"
                     /locus_tag="ACIYSB_000686"
     CDS             complement(16306..17733)
                     /gene="rmuC"
                     /locus_tag="ACIYSB_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGC5427799.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17874..18635)
                     /gene="udp"
                     /locus_tag="ACIYSB_000687"
     CDS             complement(17874..18635)
                     /gene="udp"
                     /locus_tag="ACIYSB_000687"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGC5427800.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            18897..19712
                     /gene="ysgA"
                     /locus_tag="ACIYSB_000688"
     CDS             18897..19712
                     /gene="ysgA"
                     /locus_tag="ACIYSB_000688"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGC5427801.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            complement(19752..22013)
                     /gene="metE"
                     /locus_tag="ACIYSB_000689"
     CDS             complement(19752..22013)
                     /gene="metE"
                     /locus_tag="ACIYSB_000689"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGC5427802.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            22250..23203
                     /gene="metR"
                     /locus_tag="ACIYSB_000690"
     CDS             22250..23203
                     /gene="metR"
                     /locus_tag="ACIYSB_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGC5427803.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(23091..23990)
                     /gene="bioP"
                     /locus_tag="ACIYSB_000691"
     CDS             complement(23091..23990)
                     /gene="bioP"
                     /locus_tag="ACIYSB_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGC5427804.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(24066..24866)
                     /gene="yigL"
                     /locus_tag="ACIYSB_000692"
     CDS             complement(24066..24866)
                     /gene="yigL"
                     /locus_tag="ACIYSB_000692"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGC5427805.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFQYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            complement(24874..25896)
                     /gene="pldB"
                     /locus_tag="ACIYSB_000693"
     CDS             complement(24874..25896)
                     /gene="pldB"
                     /locus_tag="ACIYSB_000693"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGC5427806.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            26007..26627
                     /gene="rhtB"
                     /locus_tag="ACIYSB_000694"
     CDS             26007..26627
                     /gene="rhtB"
                     /locus_tag="ACIYSB_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGC5427807.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(26689..27309)
                     /gene="rhtC"
                     /locus_tag="ACIYSB_000695"
     CDS             complement(26689..27309)
                     /gene="rhtC"
                     /locus_tag="ACIYSB_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGC5427808.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(27373..29202)
                     /gene="recQ"
                     /locus_tag="ACIYSB_000696"
     CDS             complement(27373..29202)
                     /gene="recQ"
                     /locus_tag="ACIYSB_000696"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGC5427809.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(29335..30204)
                     /gene="pldA"
                     /locus_tag="ACIYSB_000697"
     CDS             complement(29335..30204)
                     /gene="pldA"
                     /locus_tag="ACIYSB_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGC5427810.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            30369..30836
                     /gene="yigI"
                     /locus_tag="ACIYSB_000698"
     CDS             30369..30836
                     /gene="yigI"
                     /locus_tag="ACIYSB_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGC5427811.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            30888..31778
                     /gene="rarD"
                     /locus_tag="ACIYSB_000699"
     CDS             30888..31778
                     /gene="rarD"
                     /locus_tag="ACIYSB_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGC5427812.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            31873..32253
                     /gene="yigG"
                     /locus_tag="ACIYSB_000700"
     CDS             31873..32253
                     /gene="yigG"
                     /locus_tag="ACIYSB_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HGC5427813.1"
                     /translation="MLRIFIPTSNGKISRRRYIFSFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIVLHYLVINMNCQRLRDSGFIYIKTYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            32267..32647
                     /gene="yigF"
                     /locus_tag="ACIYSB_000701"
     CDS             32267..32647
                     /gene="yigF"
                     /locus_tag="ACIYSB_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HGC5427814.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(32690..33640)
                     /gene="corA"
                     /locus_tag="ACIYSB_000702"
     CDS             complement(32690..33640)
                     /gene="corA"
                     /locus_tag="ACIYSB_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGC5427815.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(33792..33848)
                     /gene="ysgD"
                     /locus_tag="ACIYSB_000703"
     CDS             complement(33792..33848)
                     /gene="ysgD"
                     /locus_tag="ACIYSB_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGC5427816.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            34010..34774
                     /gene="yigE"
                     /locus_tag="ACIYSB_000704"
     CDS             34010..34774
                     /gene="yigE"
                     /locus_tag="ACIYSB_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGC5427817.1"
                     /translation="MAHQLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASSKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(34921..37083)
                     /gene="uvrD"
                     /locus_tag="ACIYSB_000705"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(34921..37083)
                     /gene="uvrD"
                     /locus_tag="ACIYSB_000705"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGC5427818.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRHTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(37167..37883)
                     /gene="yigB"
                     /locus_tag="ACIYSB_000706"
     CDS             complement(37167..37883)
                     /gene="yigB"
                     /locus_tag="ACIYSB_000706"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGC5427819.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(37883..38779)
                     /gene="xerC"
                     /locus_tag="ACIYSB_000707"
     CDS             complement(37883..38779)
                     /gene="xerC"
                     /locus_tag="ACIYSB_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGC5427820.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(38776..39483)
                     /gene="yigA"
                     /locus_tag="ACIYSB_000708"
     CDS             complement(38776..39483)
                     /gene="yigA"
                     /locus_tag="ACIYSB_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HGC5427821.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(39480..40304)
                     /gene="dapF"
                     /locus_tag="ACIYSB_000709"
     CDS             complement(39480..40304)
                     /gene="dapF"
                     /locus_tag="ACIYSB_000709"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGC5427822.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(40341..40544)
                     /gene="yifL"
                     /locus_tag="ACIYSB_000710"
     CDS             complement(40341..40544)
                     /gene="yifL"
                     /locus_tag="ACIYSB_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGC5427823.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            40703..40845
                     /locus_tag="ACIYSB_000711"
                     /pseudo
     CDS             40703..40845
                     /locus_tag="ACIYSB_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            41007..41327
                     /gene="cyaY"
                     /locus_tag="ACIYSB_000712"
     CDS             41007..41327
                     /gene="cyaY"
                     /locus_tag="ACIYSB_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGC5427824.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(41367..43913)
                     /gene="cyaA"
                     /locus_tag="ACIYSB_000713"
     CDS             complement(41367..43913)
                     /gene="cyaA"
                     /locus_tag="ACIYSB_000713"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGC5427825.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLENWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDLTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            44300..45241
                     /gene="hemC"
                     /locus_tag="ACIYSB_000714"
     CDS             44300..45241
                     /gene="hemC"
                     /locus_tag="ACIYSB_000714"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGC5427826.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDSRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            45238..45978
                     /gene="hemD"
                     /locus_tag="ACIYSB_000715"
     CDS             45238..45978
                     /gene="hemD"
                     /locus_tag="ACIYSB_000715"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGC5427827.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQAREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            46000..47181
                     /gene="hemX"
                     /locus_tag="ACIYSB_000716"
     CDS             46000..47181
                     /gene="hemX"
                     /locus_tag="ACIYSB_000716"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGC5427828.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGIGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPQADTPAAAPQG
                     E"
     gene            47184..48380
                     /gene="hemY"
                     /locus_tag="ACIYSB_000717"
     CDS             47184..48380
                     /gene="hemY"
                     /locus_tag="ACIYSB_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGC5427829.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFHTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            49059..50714
                     /gene="aslA"
                     /locus_tag="ACIYSB_000718"
     CDS             49059..50714
                     /gene="aslA"
                     /locus_tag="ACIYSB_000718"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HGC5427830.1"
                     /translation="MEFSFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     DYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKTLEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(50873..51736)
                     /locus_tag="ACIYSB_000719"
                     /pseudo
     CDS             complement(50873..51736)
                     /locus_tag="ACIYSB_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
     gene            complement(51925..53502)
                     /locus_tag="ACIYSB_000720"
     CDS             complement(51925..53502)
                     /locus_tag="ACIYSB_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HGC5427831.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
     gene            53812..54372
                     /locus_tag="ACIYSB_000721"
     CDS             53812..54372
                     /locus_tag="ACIYSB_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGC5427832.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            54376..57342
                     /locus_tag="ACIYSB_000722"
     CDS             54376..57342
                     /locus_tag="ACIYSB_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HGC5427833.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRAGPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPE
                     GLDRKFYEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAI
                     NPNSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALI
                     DQTSQLLPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLT
                     KMAESSPGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQ
                     RFRAGGRGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRP
                     LIGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSR
                     RLEDGKFRPLRMPGKP"
     gene            complement(57373..57753)
                     /locus_tag="ACIYSB_000723"
                     /pseudo
     CDS             complement(57373..57753)
                     /locus_tag="ACIYSB_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            complement(57900..57976)
                     /locus_tag="ACIYSB_000724"
     tRNA            complement(57900..57976)
                     /locus_tag="ACIYSB_000724"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57940..57942),aa:Pro,seq:tgg)
     gene            complement(58019..58105)
                     /locus_tag="ACIYSB_000725"
     tRNA            complement(58019..58105)
                     /locus_tag="ACIYSB_000725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58069..58071),aa:Leu,seq:cag)
     gene            complement(58126..58201)
                     /locus_tag="ACIYSB_000726"
     tRNA            complement(58126..58201)
                     /locus_tag="ACIYSB_000726"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58166..58168),aa:His,seq:gtg)
     gene            complement(58260..58336)
                     /locus_tag="ACIYSB_000727"
     tRNA            complement(58260..58336)
                     /locus_tag="ACIYSB_000727"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58300..58302),aa:Arg,seq:ccg)
     gene            complement(58439..59824)
                     /gene="yifK"
                     /locus_tag="ACIYSB_000728"
     CDS             complement(58439..59824)
                     /gene="yifK"
                     /locus_tag="ACIYSB_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGC5427834.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(60015..60755)
                     /gene="wecG"
                     /locus_tag="ACIYSB_000729"
     CDS             complement(60015..60755)
                     /gene="wecG"
                     /locus_tag="ACIYSB_000729"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGC5427835.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIIMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(60758..62110)
                     /gene="wzyE"
                     /locus_tag="ACIYSB_000730"
     CDS             complement(60758..62110)
                     /gene="wzyE"
                     /locus_tag="ACIYSB_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGC5427836.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(62107..63186)
                     /gene="rffT"
                     /locus_tag="ACIYSB_000731"
                     /gene_synonym="wecF"
     CDS             complement(62107..63186)
                     /gene="rffT"
                     /locus_tag="ACIYSB_000731"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGC5427837.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LFFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(63183..64433)
                     /gene="wzxE"
                     /locus_tag="ACIYSB_000732"
     CDS             complement(63183..64433)
                     /gene="wzxE"
                     /locus_tag="ACIYSB_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGC5427838.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(64435..65565)
                     /gene="rffA"
                     /locus_tag="ACIYSB_000733"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(64435..65565)
                     /gene="rffA"
                     /locus_tag="ACIYSB_000733"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGC5427839.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(65570..66244)
                     /gene="rffC"
                     /locus_tag="ACIYSB_000734"
                     /gene_synonym="wecD"
     CDS             complement(65570..66244)
                     /gene="rffC"
                     /locus_tag="ACIYSB_000734"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGC5427840.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(66222..67103)
                     /gene="rfbA"
                     /locus_tag="ACIYSB_000735"
     CDS             complement(66222..67103)
                     /gene="rfbA"
                     /locus_tag="ACIYSB_000735"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGC5427841.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(67122..68189)
                     /gene="rffG"
                     /locus_tag="ACIYSB_000736"
     CDS             complement(67122..68189)
                     /gene="rffG"
                     /locus_tag="ACIYSB_000736"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGC5427842.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(68189..69451)
                     /gene="wecC"
                     /locus_tag="ACIYSB_000737"
     CDS             complement(68189..69451)
                     /gene="wecC"
                     /locus_tag="ACIYSB_000737"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGC5427843.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(69448..70578)
                     /gene="wecB"
                     /locus_tag="ACIYSB_000738"
     CDS             complement(69448..70578)
                     /gene="wecB"
                     /locus_tag="ACIYSB_000738"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGC5427844.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDKLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(70634..71680)
                     /gene="wzzE"
                     /locus_tag="ACIYSB_000739"
     CDS             complement(70634..71680)
                     /gene="wzzE"
                     /locus_tag="ACIYSB_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGC5427845.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(71692..72795)
                     /gene="wecA"
                     /locus_tag="ACIYSB_000740"
     CDS             complement(71692..72795)
                     /gene="wecA"
                     /locus_tag="ACIYSB_000740"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGC5427846.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(73035..74294)
                     /gene="rho"
                     /locus_tag="ACIYSB_000741"
     CDS             complement(73035..74294)
                     /gene="rho"
                     /locus_tag="ACIYSB_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGC5427847.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(74379..74480)
                     /gene="rhoL"
                     /locus_tag="ACIYSB_000742"
     CDS             complement(74379..74480)
                     /gene="rhoL"
                     /locus_tag="ACIYSB_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGC5427848.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(74621..74950)
                     /gene="trxA"
                     /locus_tag="ACIYSB_000743"
     CDS             complement(74621..74950)
                     /gene="trxA"
                     /locus_tag="ACIYSB_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGC5427849.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            75081..76346
                     /gene="rhlB"
                     /locus_tag="ACIYSB_000744"
     CDS             75081..76346
                     /gene="rhlB"
                     /locus_tag="ACIYSB_000744"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGC5427850.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            76482..77966
                     /gene="gppA"
                     /locus_tag="ACIYSB_000745"
     CDS             76482..77966
                     /gene="gppA"
                     /locus_tag="ACIYSB_000745"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGC5427851.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(78013..80034)
                     /gene="rep"
                     /locus_tag="ACIYSB_000746"
     CDS             complement(78013..80034)
                     /gene="rep"
                     /locus_tag="ACIYSB_000746"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGC5427852.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            80251..80699
                     /locus_tag="ACIYSB_000747"
                     /pseudo
     CDS             80251..80699
                     /locus_tag="ACIYSB_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            80898..81179
                     /gene="ppiC"
                     /locus_tag="ACIYSB_000748"
     CDS             80898..81179
                     /gene="ppiC"
                     /locus_tag="ACIYSB_000748"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGC5427853.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(81266..82741)
                     /gene="ilvC"
                     /locus_tag="ACIYSB_000749"
     CDS             complement(81266..82741)
                     /gene="ilvC"
                     /locus_tag="ACIYSB_000749"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGC5427854.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     INLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNGQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            82891..83784
                     /gene="ilvY"
                     /locus_tag="ACIYSB_000750"
     CDS             82891..83784
                     /gene="ilvY"
                     /locus_tag="ACIYSB_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGC5427855.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNHK"
     gene            complement(83836..85380)
                     /gene="ilvA"
                     /locus_tag="ACIYSB_000751"
     CDS             complement(83836..85380)
                     /gene="ilvA"
                     /locus_tag="ACIYSB_000751"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGC5427856.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTYWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(85383..87233)
                     /gene="ilvD"
                     /locus_tag="ACIYSB_000752"
     CDS             complement(85383..87233)
                     /gene="ilvD"
                     /locus_tag="ACIYSB_000752"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGC5427857.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(87298..88227)
                     /gene="ilvE"
                     /locus_tag="ACIYSB_000753"
     CDS             complement(87298..88227)
                     /gene="ilvE"
                     /locus_tag="ACIYSB_000753"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGC5427858.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(88247..88510)
                     /gene="ilvM"
                     /locus_tag="ACIYSB_000754"
     CDS             complement(88247..88510)
                     /gene="ilvM"
                     /locus_tag="ACIYSB_000754"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGC5427859.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(88507..90153)
                     /gene="ilvG"
                     /locus_tag="ACIYSB_000755"
     CDS             complement(88507..90153)
                     /gene="ilvG"
                     /locus_tag="ACIYSB_000755"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGC5427860.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIHGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(90156..90206)
                     /gene="ilvX"
                     /locus_tag="ACIYSB_000756"
     CDS             complement(90156..90206)
                     /gene="ilvX"
                     /locus_tag="ACIYSB_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGC5427861.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(90293..90391)
                     /gene="ilvL"
                     /locus_tag="ACIYSB_000757"
     CDS             complement(90293..90391)
                     /gene="ilvL"
                     /locus_tag="ACIYSB_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGC5427862.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            90744..92264
                     /gene="yifB"
                     /locus_tag="ACIYSB_000758"
     CDS             90744..92264
                     /gene="yifB"
                     /locus_tag="ACIYSB_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGC5427863.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVIGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(92289..92627)
                     /gene="maoP"
                     /locus_tag="ACIYSB_000759"
     CDS             complement(92289..92627)
                     /gene="maoP"
                     /locus_tag="ACIYSB_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGC5427864.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            92746..93585
                     /gene="hdfR"
                     /locus_tag="ACIYSB_000760"
     CDS             92746..93585
                     /gene="hdfR"
                     /locus_tag="ACIYSB_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGC5427865.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(93681..93756)
                     /locus_tag="ACIYSB_000761"
     tRNA            complement(93681..93756)
                     /locus_tag="ACIYSB_000761"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93721..93723),aa:Trp,seq:cca)
BASE COUNT        22577 a        25661 c        24024 g        21500 t
ORIGIN      
        1 ttctttatgc ttatttcagc gtcggtttgt cggttaaatg ggcgatttct cgggcgaaat
       61 tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg
      121 acatcttcat aatcagcttg atcataaaac ggtcgtacca gcgataacgt gggtaacgca
      181 gcgccccggc aatgaccgcg cagcgatcgg gacgccattg cgagttcatc agaaacttgc
      241 gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc agattcacgg
      301 agtaaaaggc gctcggcatc gaattcagcc gcgtcgcatg ttttttgaca aattcctgga
      361 acgctgaatg gtagtgacca tagcgaatag aagcaccaat gaccacacgg tcatagtttt
      421 cccactgtgg ttcttcaatg cggtgcacat tggcgacatc cgcctggatc cccagttctt
      481 tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa
      541 gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg taaagagcac
      601 cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga tccatttagc
      661 caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc ccaggttatt
      721 caatgtcgca acaaccgacg caaaggcaga aaagtcatcc acgcccgtgg cgataatcgc
      781 cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa cggcttcgag
      841 gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt tcggatgcac
      901 cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga tcaccttcag
      961 gccaccgccc gttgacccgg cgcaaccgcc gataaaggct gaacataaaa gcagtaccgg
     1021 caaaaagagc ggccagcggg caatgctgtc agttgtaaac ccggcggttg tcgccatcga
     1081 caccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga cattatgaaa
     1141 ccacagtacg agggtacaaa taaccaccag cgtaaactgt acgccgataa acatgcgaaa
     1201 ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa agtgcagacc
     1261 gtagttacag ccggagatca gcaggaagat agcaatgata gtgttaatag tcgggctatc
     1321 gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag tcgcaaagct
     1381 atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg cacaggcgac
     1441 ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc gcgggcgcat
     1501 tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc cacccacgcc
     1561 gaggataggc agtatcgcaa ccgccaacac gatgatcccc atcccgccaa accattgcag
     1621 catctggcga taaaagagga tggcgtgagg gagcgaatcc agccccacca gcgtagtggc
     1681 tcccgtagtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg
     1741 gctttccgag aagataaaag ggagcgcacc gacgctaccc agcacggtcc agaacagcac
     1801 cactatcaga aacccctcac gggatttaag ttcgcctttc tctttgcggt tcggccacca
     1861 cagcatagag ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc
     1921 atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa ataagatgac
     1981 cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt ccttagttct
     2041 gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa
     2101 tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac ccgcagcaaa
     2161 acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag caacgcttca
     2221 atgccggtta actgatgata ttcacattgc aaagtatatt cggttaatgg cgtcttgcgt
     2281 tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt cactaaccca
     2341 ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagcggtaat ttccccgacg
     2401 ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcccgccgg ctccccgtcg
     2461 tcagagaaac ccagctgttg agaatcatcc ggcgcacccg cgacccacgc cacgcaatgg
     2521 tgccgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc cgcctcaacg
     2581 ccatcggtat gcgccaacat cgttatgaag cggctctttt tgatctcttc aacgaccgtg
     2641 accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc cgggtcatgt
     2701 tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat acgaatgccg ccgaacggtt
     2761 tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca cgccacggtg
     2821 ccagtagcga ttcgatgaag tagatacccg gttcgatggt taacaccatg cccggctgga
     2881 gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgc gtaccgctat
     2941 catcctgcat aaaaccagcg acgtcatgca cctgcaggcc cagcggatgg ccgataccat
     3001 gcggcataaa cggcccggta agatcgtttt cgaccatcgc ttcttcactc atatcggtga
     3061 tgatttgatg tttacgcagc agtttggcga tgcgctgatg gaactggatg tggtaatcca
     3121 catagctgac gcctgctttc atggtggcga tcagcgccag ttgttcatca ttaacgtcct
     3181 tcaccagctg cgcgtagtcg ttgtcacttt ttgccgacca ggtacgggtc aggtcagccg
     3241 catagccgtt atattctgcc ccggcatcca gcaggaagct gcgcatctct tccggtgcct
     3301 gatggtccag tttggtgtaa tgcagtaccg cagcgtgttc gttaagcgcc acaatgttgc
     3361 tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcaatg ttgatatcga
     3421 actcgctcat gccagaacgg aacgcttctt ctgccgcgcg gtgaccgttg accgccattt
     3481 tctgcgcttc acgcatacag gccagttcgt actcagtttt gaaggagcga tagtaatgca
     3541 ggtagtcgat aacccctttc gggttgatgt tgctggcctc aatacccagt tgcagcgcac
     3601 gttccggcac cggaccgata taaccgatat tgccgcgcgc agcaggcagc aggctaccaa
     3661 tgccatcggc tttcggcagc gcgatcactt ccacatcttc agtccagaaa gaggtcggca
     3721 gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc ggcttgttca
     3781 cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac gctttgaatt
     3841 gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacattgaac agctcgccgg
     3901 agtgaataag taacgcatcc agcttgaagc gcgccagcgc atcgcgagtc cgttcttgta
     3961 aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac ccttctgttt
     4021 ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga tttctgccga
     4081 gcgtgatcag atcggcattt ctttaatctt ttgtttgcat atttttaaca caaaatacac
     4141 acttcgactc atctggtacg accagatcac cttgcggatt caggagactg acatgcttta
     4201 caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac tggtatttga
     4261 tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg gcgaggccat
     4321 cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga acaaagcagc
     4381 ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca
     4441 gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag atctgccggt
     4501 gccgaccatt gctgccgtca atggctatgc gctgggcggt ggctgcgaat gcgtgctggc
     4561 gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa ccaaactggg
     4621 catcatgcct ggctttggcg gttctgtacg tatgccacgt atgctgggcg ctgacagtgc
     4681 gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga aaatcggtct
     4741 ggtggatggc gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg ttttacgcca
     4801 ggccattaac ggcgacctcg actggaaagc aaaacgtcag ccgaagctgg aaccactaaa
     4861 actgagcaag attgaagcca ccatgagctt caccatcgct aaagggatgg tcgcacaaac
     4921 agcggggaaa cattatccgg cccccatcac cgcagtaaaa accattgaag ctgcggcccg
     4981 ttttggtcgt gaagaagcct taaacctgga aaacaaaagt tttgtcccgc tggcgcatac
     5041 caacgaagcc cgcgcactgg tcggcatttt ccttaacgat caatatgtaa aaggcaaagc
     5101 gaagaaactc accaaagacg ttgaaacccc gaaacaggcc gcggtgctgg gtgcaggcat
     5161 tatgggcggc ggcatcgctt accagtctgc gtggaaaggc gtgccggttg tcatgaaaga
     5221 tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca
     5281 gcttgagcgc ggcaagatcg atggtctgaa actggctggc gtgatctcca caatccaccc
     5341 aacgctcgac tacgccggat ttgaccgcgt ggatattgtg gtagaagcgg ttgttgaaaa
     5401 cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc aggataccgt
     5461 gctggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc tggaacgccc
     5521 ggaaaacttc tgcgggatgc acttctttaa cccggtccac cgaatgccgt tggtagaaat
     5581 tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct gggcgagcaa
     5641 gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta accgcgtgct
     5701 gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt tccgcaagat
     5761 tgacaaagtg atggaaaaac agtttggctg gccgatgggc ccggcatatc tgctggacgt
     5821 tgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct tcccgcagcg
     5881 gatgcagaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc gctttggtca
     5941 gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc cgaagaaaga
     6001 agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc gcgatttcag
     6061 cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg tgcgctgtct
     6121 ggaggaaggc attatcgcca ctccggcgga agcggatatg gcgctggtct acggcctggg
     6181 cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta gcgcaaaata
     6241 cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc cggaaggtct
     6301 gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag cccgtccggt
     6361 tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg tcgatgcaat
     6421 tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg cagaagatct
     6481 ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag cggcggccct
     6541 cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta atatcgcccg
     6601 taacgcggcg ctgctggcag aagtaccaca ctctgtcccg gcggttaccg ttaatcgctt
     6661 gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga ctggcgatgc
     6721 gcaggcatgt ctggttggcg gcgtggagca tatgggccat gtgccgatga gtcacggcgt
     6781 cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga tgggcttaac
     6841 ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg cctttgccgc
     6901 gcggtcacac gcccgcgcct gggccgccac gcagtcggcc gcatttaaaa atgaaatcat
     6961 cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg acgaagtgat
     7021 tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg atccagtaaa
     7081 cggtatggta acggcgggca catcttctgc actttccgat ggcgcagctg ccatgctggt
     7141 gatgagtgaa agccgcgccc atgaattagg tcttaagccg cgcgctcgtg tgcgttcgat
     7201 ggcggtcgtt ggttgtgacc catcgattat gggttacggc ccggttccgg cctcgaaact
     7261 ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaaa tgaacgaagc
     7321 ctttgccgcg cagatcctgc catgtattaa agatctggga ctaattgagc agattgacga
     7381 gaagatcaac ctcaacggtg gcgcgatcgc gctgggtcat ccgctgggtt gttccggtgc
     7441 gcgtatcagc accacgctgc tgaatctgat ggaacgcaaa gacgttcagt ttggtctggc
     7501 gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg tttaaccact
     7561 tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc cggcaccaat gtaggcctga
     7621 taagacgcgc cagcgtcgca tcaggcatca tgcaccaatt gccggatgcg gcgtaaacgc
     7681 cttatccggc ctacggttcg gactatttgt aggcacgata agacgcgaca gcgtcgcatc
     7741 gggcatggtt tgccagatac gtcatagatc taaatgccac tgaataacag tttagttgcc
     7801 gttcttcccg cctgtcaggg gcgggttttt ttatatctca gataaatgca aacgcatcgc
     7861 caaacaggcg atcttcccgc gcattacgct cactgcaaaa cagatcgcgg gcaattttcg
     7921 ccatctcaaa acgtccggca atatagatat catgctctgc cagcgtaccg tgatcctgca
     7981 ataccgccgt taacacggtg ccagtacgcc cacgccagcc cgcttccggt tgttcaacca
     8041 ccggcaccac ttgcagacca ggatgcttca acgaaagcgc ctcaagctcg cagagatcat
     8101 acagatgctg ctcttcacgc ccgccccagt aaatggtgat atcacggttt gggttacgcg
     8161 ccaacgctgt cagcaaaatc gagcgggcat aagagaaccc ggtgccgccc gcaatcaaaa
     8221 tcatcggacg ctcttcatca tcgcgcagcc acgcttctcc gtggggaatg tcgaccacga
     8281 tttgatgatc tttgaggatg cggtccatga ctgctttcgc gtaaaggttg atttcagaag
     8341 cgccaatatg cagctcgata aacccttttt catccggcgt cgaagccatt gagaacggac
     8401 gtttgtcgcg ctcatccatc actaccatca aatactgacc agcacgaaaa gaaaaggccg
     8461 cgtctggcac gatgcggaca cgatatacgg tatccgtgat agcttctacc gaggtcactt
     8521 tacagcttaa ggttgtcatg cgctttctct gtcggatcga taaatagggc aaaacaaacg
     8581 cgcatcaggc gcttttaccg ttgttaaaaa tagccagttc atcccagatg gcgtcaatat
     8641 gcgcgacaac atctggatct tttttgatgg gacgtcccca ttcacgctgg gtttcccccg
     8701 gccatttatt cgtggcatcc agccccattt ttgaacccag cccggagaca ggcgaggcaa
     8761 aatccagata atcaataggc gtattttcta ccagaacagt atcccgcgcc gggtccatac
     8821 gggtggtaat cgcccaaatc acatcgttcc agtcgcgtgc gttaacgtca tcatcgcaaa
     8881 cgatcacaaa tttagtgtac ataaactggc gtaagaacga ccagacgccc atcatgacgc
     8941 gcttcgcgtg tccggcgtac tgttttttga ttgtcactac cgccaggcga taagagcagc
     9001 cttccggcgg caggtaaaaa tcgacaattt ccgggaactg tttttgcaga atcggcacaa
     9061 acacttcgtt cagtgcgaca cccagcaccg cgggctcatc tggcggacgc ccggtatagg
     9121 tggaatggta aatcgcatct tcacgctggg taatatgcgt cacggtaaat accgggaaac
     9181 tatcgacttc attatagtaa ccggtgtggt cgccatacgg cccttccggc gcagtttcgc
     9241 cttgttcgat atacccttcc agcacaatct ccgcactggc gggcacttca agatcattgg
     9301 agatacactt caccacttcg gtcttggtgc cacgtagcaa tccggcaaac gcatactctg
     9361 aaagcgtatc cggaacggga gtgactgcac cgagaatcgt ggcgggatcg gcacccagcg
     9421 ccacagaaac cgggaaacgt tcgcccggat gcgccgcaca ccactcctga taatccagcg
     9481 cgccgccgcg atgcgacagc cagcgcataa tcagtttgtt tttaccaatc agctgctggc
     9541 gataaatgcc cagattctgc cgctctttat gtgggccgcg cgtcactgtc agcccccagg
     9601 taatcagcgg cgcggcatct tccggccagc aggtcataat gggaatgcga ttgagatcga
     9661 cgtcatcgcc agagacgatt ttttgttggc agggcgcacc acgcagccgc tttgtcggca
     9721 tgttcaatac ttgcttaaac tgcggcagtt tatcaaacag gtcgcggaaa ccttttggcg
     9781 gctccggctc tttcagaaac gccaataatt taccaacttc acgcagcgcc gaaacatctt
     9841 cctgccccat gcccatcgcc acgcgctttg gcgtaccgaa caggttgcac agcaccggca
     9901 ttgagtagcc tttagggttt tcgaacaaca gcgcaggccc accggcacgc aaagtgcggt
     9961 cagcaatttc agtgatttcc agatgcggat ccaccgggag cgtgatacgt tttagctcac
    10021 cctgctgttc aagcagcgtc aagaagtcgc gtaaatcgtt atatttcatg gcgtccattg
    10081 tagcctctta atctgcgccc attatacggc gttcatcttt gcgatgctgt aaatttgtta
    10141 aattagcgtg aactctgacg gtataacgca aaccggggaa tataattaac ttagcgtaaa
    10201 gcttttgcta tccttgcgcc ccgattaaac ggataagagt cattatgcaa tcctggtatt
    10261 tactgtactg caagcgcggg caacttcaac gtgcccagga acacctcgaa agacaggctg
    10321 tgaattgcct ggcaccgatg atcaccctgg aaaaaatcgt gcgtggaaaa cgtactgcag
    10381 tcagtgagcc attgttcccc aactacctgt ttgtggaatt tgacccagaa gtgattcata
    10441 ccacgactat caacgcgacc cgcggtgtca gccacttcgt gcgctttggc gcgtcgccag
    10501 cgatagtccc atcggcggtt attcatcagc tatcggtata taaaccgaaa gacattgtcg
    10561 atccggcaac cccttatccg ggtgataagg tgattattac cgaaggcgcg ttcgaaggct
    10621 ttcaggccat tttcaccgaa cccgatggtg aggctcgctc catgctattg cttaatctta
    10681 ttaataaaga gattaagcac agtgtgaaga ataccgagtt ccgcaaactc taaaacgcaa
    10741 tcccaaacag tgttttgaca ttagcatccg tggtggcagc cagccatgcg gcatcttctc
    10801 cacgccagtg cgcaatacgt tgcaaaatat ggggcagatg ggctggctcg ttgcgccggg
    10861 atgatggctt tggcgtgaga tcgcgaggga gcagatacgg cgcatcagtt tcgatcagta
    10921 atttttccgc cggaatcaac ggcaaaagtt cccgcagctc cagtccgcgt cgttcatcgc
    10981 aaacccaacc ggtaatgccg atataaattc catgcgccac gcacgcctgc atctcttcgc
    11041 gtgtgccggt aaagcaatga agaaccgcac caggcagttt atccagccac ggctccagca
    11101 atgtcataaa ccgctcgtgg gcatcgcgac agtgcataaa taccggcatg tttaaatctg
    11161 cggcaatgcg tagctgggca acaaaagcgc gttcctgctc ttccggcgtc gaaaagttgc
    11221 ggttaaagtc gagaccacat tcaccaatcg ccaccacttc tggctgcgcg gccagctcaa
    11281 taatcgcttc ttcagtcgca gcttgccact ggctgctgtc gtgaggatgt acgcccgccg
    11341 ttgaccaaca ggacgaatac tgacgcgcca gcttttgcgc ctgctggctt tcacgcaggt
    11401 tagtgccggt gatgagtagc ccattaactc ccgcgtcaaa agcgcacgct acaacatcat
    11461 cacggtcttt cgcaaattgc gaactggtca aattaacgcc gatatcaaac atcctgtact
    11521 ccatatgaca accgccctga cgggcggttg aatttattct tcagtttttt cgctttctgc
    11581 ttcagcgtcg ttttcctctt cccgatttcg ccctttacca acgtaaaagc gtgagaagaa
    11641 gacaccgatt tcaaacagac agtacatcgg gatcgccaac agcgtttgcg agaagacatc
    11701 cggcggcgtc agcaacatcc cgacaacgaa tgcaccaacc agcacatacg ggcgtttttt
    11761 gcgtaagtct tctggcgagg taatccccat ccagcacagc agcacaattg ctaccggcac
    11821 ttcaaaggag acaccaaacg ccataaacag cgccataacg aagcttaaat agctggcgat
    11881 gtcggtggat acctgcaccc cttccggcgc ggtattggca aggaagccaa atgccagcgg
    11941 aaagaccaca aagtaggcga atgccatgcc gatataaaac agcagagagc tggaaaccag
    12001 cagcggcacc accaggcgac gttcatgctt atacagcgct ggggcgataa atgcccacac
    12061 ctgatagaga atcaccggcg ctgacagaat cagcgacacc ataaaggtca gcttgatcgg
    12121 cgtaaagaac ggcgaggcca cgtcggtggc gatcatcgtt gaaccttgcg gcaactgctt
    12181 gatcaatggc gcggatacca ggtgatagat gtcattggcg aaatagacca gacacaggaa
    12241 tatcacgatc accgcgataa tgcagttcag cagacgctta cgcagctcaa tcagatgcgt
    12301 gataagcggt tgagtatctt ctacagacat gtttacggtt tatcactcga cgaaggggaa
    12361 ggtgcagcgg ttttcggttc agcgtccgca gcaggtttta ccaccggctc tggcgtggtt
    12421 tctggcttct gttccggcga actggcctgc gtttgtgcag cggcaggcgt tacgccctca
    12481 tgcgcagctt cattatcttt caccaccggg ttatggatgg tgtgcgcttc atcgctcgcc
    12541 ttttcaggat cgtttgcaac gtaggaacgc ttcatcgact ccgcggcctg gcgtagttca
    12601 tccatcgacg ctttcagttc gggcgtcagg ttagtgaggc tcgccttttc aacctttttc
    12661 agactgtcct gaaactcctg gagttttaac tcctgggtca gttcgttctg caccgttgtc
    12721 gccagtgaac gcaacgcgcg aatccagccc gctaccgttt ttaccgccac aggcagtcgt
    12781 tgcggcccca gaacgacgag gccgatgatg aacaccaata gcagttcgct aaaaccgata
    12841 tcaaacacgg attacacctg ctctttatcg tggcgcttcg cgtcttctgt tttagcctgt
    12901 tcctgattcg tatccgcctg cttatcggcg atagttttcg cagtaaaatc agcatcctga
    12961 ctggttttat cctgctttgg ttcatcatcg ctcattgctt ttttaaagcc tttgatcgac
    13021 gcaccaagat cggaaccgat ggagccgagc tttttggtgc caaaaagcag tacaacgatg
    13081 acggcaataa tcaataactg ccaaatactg ataccaccca tacatgttcc tctgtggtag
    13141 atgatgatta aacaaagccg cattatacgt tacacggccc gccttgagcg atgaaaaaat
    13201 cagcgtgttt tgcgccaacc gacaaaccag gcgatcagac cacctgccat taaccagccg
    13261 ggcatcagcc cccattcagg tcggctgacc aacaagaatg tgccacttaa tactaacgta
    13321 gcgccaattc cgagaaaata acgcgattgt ccctgacgta catgatttga ctgaagctcg
    13381 cgggcaatct tatcaacact gtgctgtaaa tacttgccct ggcgcaaact gtcgtaaacc
    13441 aattcaggca gttctggcat tttttcgacc cagaacggcg ctttttcttt aaatgctctc
    13501 accagcgcag gaataccgac ctgatcttta atccacgact ccaggaaagg cttcgccgtt
    13561 ttccataaat cgagttgcgg ataaagctgg cgtcctaccc cttcgacgta gagcagggtt
    13621 ttctggagta acaccagttg cggctgcact tccatattga agcgacgcgc cgtattaaac
    13681 agatttaaca gtacatgtcc aaacgaaatt tcggccagcg gtttctcaaa gataggttca
    13741 cagaccgtac gaatggcaaa ttcgaactct tcaacgttgg tatctggtgg cacccagcca
    13801 gaatcgacgt gtagctctgc cactttgcga tagtcgcgat taaagaaggc gataaagttt
    13861 tctgccagat agcgtttatc ttctttgttt agcgagccaa caatcccgca atcaatgccg
    13921 atatatttcg ggttttccgg gtgttcatag cttacgaaga tgttgccagg gtgcatatcg
    13981 gcatggaaaa agctgtcgcg aaagacctga gtgaagaaca cctgcacgcc gcgttccgcc
    14041 agcaatttca tgttagtgcc gtttttctcc agcgccgcaa catcagacac cggaatgccg
    14101 taaatgcgct ccatcaccat catcccttca ctacaatagt cagggtaaac ttccgggatg
    14161 tagagcatcg ggctgtcttc aaaattgcgc cgaagctgaa tggcgttggc agattcccgc
    14221 agcaaattca gttcatcaat caatgtcttt tcgtactcgc gcaccacttc ggttgggcgc
    14281 agacggcgac catccggcag caaacgcggc acccagcgag ccagacggta gataagtttc
    14341 agatccgctt taataaccgg caaaatatcc gggcggatga ctttaatcac cacctcttta
    14401 ccattcgatt tcaatcgcgc ggtatgaacc tgggcgatag aagcagaagc cagcggcttg
    14461 atttcaaaat cgtcaaacca cgcttctacc ggcaagccgc ccattgcagc ttcaatctgc
    14521 tgcttcgcca gcttgccatc aaacggagca actttgtcct gcaataacgc cagctgatcg
    14581 gcaatatgcg gtggaaaaag atcgcggcgg gttgataaca tttgcccgaa cttgatccaa
    14641 accggcccca gttcttgcag ggccagtcgt agtcgctcac ctaaaagttt gtctttatgc
    14701 cgatttggca tccagaataa tgagtatcgc catagccgta gcggcagggt gatacgcatt
    14761 ttggggatca gttcatcaag tccgtagctt aaaaaagtgc gaatgatgaa atataggcgc
    14821 cgtacttcac ctggcgtcat ttagcctcca gtttttccag ccgtttggtc agggcatcaa
    14881 cagcacgctc gacggcagcc gtctcttccg caaaccaggc cacttcaagc ggaccgggtg
    14941 ccatacgcca ctcttcagta atggcttccg ccacataacg ttgctggcgc ttaatgccgt
    15001 gatgcaggaa ctttgcgcct ccgcgcatgg ctttgctgat tccttcagcg gcgatatcac
    15061 cggtataagg ggccagcagt tccgcagggt cgaactctgc cagatctgcc agcgcaacga
    15121 agttttgcac cacctgaata tcgccctgca cttccagctc accactgcga atcagtgcgg
    15181 taagctgctg gcgatcgcga agtttcggca acacactggc gtaggcgata acggtgcagt
    15241 cagcatcgcc tgcccattcg cccagtacat caacctggcg ttcgctgaac accagaatca
    15301 atgacgtcga aaagcctttt acctccacgc gcaatacttt acccagcaga cgcgagcggg
    15361 ccgttttcag cgcgggtgag cgatacagga aggtgttgag cagactttca attcctgccg
    15421 tcactaaagg tttaaaaggc atttccggtc tcctgtcaga acttataacc acgatgcagc
    15481 gccacaaccc ctgccgtcag attgtagtag tcgacacttt cgaatccggc atcctgcatc
    15541 atggctttca gggtatcctg atcgggatgc atacggatgg attctgccag ataacggtag
    15601 ctgtcggcgt cgttcgcgac cagtgagcca atacgcggca gcacatggaa ggagtatgca
    15661 tcataggctt tgctcagcgg ctcgataatt ggcttcgaga actcaagcac cagcaggcgg
    15721 ccgccgggtt tcagcacgcg atacattgaa cgcagtgctt tatctttgtc ggtgacgtta
    15781 cgcagaccaa acgaaatggt gatgcaatca aaggtgttat ccgggaacgg cagcgcctca
    15841 gcgttcgcct gaacatactc aacgttgcca atcacaccga tattacgcag cttctcgcgg
    15901 cccattttga gcatggattc attgatatca gcaaggacca ctttgccagt ttctccgacc
    15961 aggcgggaga atttcgctgt caggtcgccg gtgccaccag ccagatccag cacggtctgc
    16021 ccacggcgta cgccgctgca atcaatcgtg aatcgcttcc acaaacgatg aataccaaat
    16081 gacatcaaat cattcatgac atcgtatttt gatgccacgg aatggaaaac gtgggcgacc
    16141 atatccgctt tttgttcctt cgcgacggtc tgaaaaccaa agtgcgtcgt ttcttgtgac
    16201 ttatccacca tctcaatgcc tgctcatcaa aaaattgttc cagaagtgta acagattggg
    16261 cgtcgatgcc ctagatttct acccggctta actactccca atgggctagc gcgactgctg
    16321 attatattca tcatcgcgtt gataagcttc atcattcggc tgctccggaa ccgaccgaag
    16381 tcgatactct tcatcctggc tcaccgcctg ttcagccaaa tccggattaa tctcgcgttt
    16441 aatttctact cctaaaccgc gaaacgcttc tgcctgcgcc agcacatttc cacgccctga
    16501 agagagtttt ttcattgcct gccgataatt atcctgcgct ttgtcgagac tttgaccaat
    16561 cgcggacatg tcatcgatga acaaacgcat cttgtcgtac agcttgctgg cacgatcggc
    16621 gatttgctgg gcgttgcggc tttgatgctc ataacgccac aggttggcga tagtgcgcag
    16681 cgccaccagc agcgtagtcg ggctaaccag catgatgttg tttttcaacg cttcggtgat
    16741 cagctccggc tggcggtcaa gcgccagtaa aaaagcgggt tcaacgggaa taaacatcag
    16801 cacgtaatcc agagttcgca gccccggcag ctgttgataa tctttgcgtc ccagcaaacg
    16861 gatatggtta cgcaccgacg cgatatgttc ctgtagcgcg ctttcgcggg tgtagtcgtc
    16921 ttcggcgtta aaatagcgtt cataggcgac cagcgtcatt ttggcgtcga tcaccacatc
    16981 ttttccctgc ggcaggcgca cgatgacatc cggctgcatc cgcgagcggg cgtcattttc
    17041 gatgctgacc tgggtttcat attcataccc ttcacgcaga ccggaagcct ccagcacccg
    17101 cgtcaatact acctcgcccc agttgccctg ggttttattg tcgcctttca gcgcgcgcgt
    17161 caggttgatc gcttcctggg ccatttgcgc gttgagttgc tggagattgc gaatttcgtg
    17221 ggtcagggta tggcgttctt gtgcttcttt accgaagctg tcctgaacct gacggcggaa
    17281 accgtccagt tgttcacgta gcggcgacaa caggctgttc agactctgac ggttttgctc
    17341 atcaacccgg cgattgctgt gctcaaaaat acggttggcg aggttttcaa actgctcact
    17401 gaggcgctgc tcgctgttaa tcatctggcg aattttatcg tcagcatgtt gctgtgcggc
    17461 ttccatccgc gtggttactt cacgcagatc ggcctccaga gaggtgttaa tactttgcag
    17521 gctgcgcact tcgttattga gtaactcgca ctctgcacgc cagtgctcgc tttgggtaat
    17581 ttgttgtttt gccgcgctta actccgcgac catctcttca cgttcagcta attgctcggc
    17641 tttttgctgc gcatgttgat aactggcaaa cagccagcca attgccacac ccaccaacgc
    17701 aataactgcg taaaccatga ttgagaaatc cacaacgcct cctgacgcaa caacttaccc
    17761 gcacaaaata gaattgtgct gtacaaacgt ccagttggaa aggcatttcc tgcgaggcgc
    17821 tacgcaaaaa tacaaaaggc cgaacgcgtc ggccttcaga caggagaaga gaattacagc
    17881 agacgacgcg ccgcttccac cacgattttc accgcatggc tttcggtttg tttcatcgtc
    17941 tcagcattcg ggatctcttg ctgggtgcgg ttaacgataa cacccgctac cataccggca
    18001 cgcaggccct gacttgcaca catggtcagc agggttgcag attccatttc atagttcatt
    18061 acgcccatcg cctgccactc ttccatagaa cctttaaagt gacgaactac gcgaccagag
    18121 taagtatcgt aacgttcctg acctgggtag aaggtatcag aagaagctgt cacgccaacg
    18181 tgagttgtcg cgccaatgga tttcgcagct tcaaccagcg cagtcgtaca ttcgaaatca
    18241 gcgacagccg ggaattccag cggtgcgaag tgcaggctcg cgccatccag acggacagac
    18301 gccgtggtaa ccaggacatc acccacatta atatgcggct gaatagcgcc cgttgtaccg
    18361 atacgcagga aggtgcgaat gcccagctgt gccagctctt caacagcaat agaggtagac
    18421 gggccgccga taccggtaga gcagacgata acaggtttac catccagctc tgcacgccag
    18481 gtagtgaatt cgcggtgaga tgccagctta accggcttat ccatcagcgc ggcgatcttt
    18541 tccacacgat ccgggtcgcc agggacgatg gcaagcgtag ccccttgtaa atcgttttta
    18601 gtgaggccga gatgaaaaac atcagacttg gacatataca actcctctgt gaatcggttt
    18661 agtcagaaga agcaaaaaga cactttaccg aagggtttaa cattttttcg tgatactcat
    18721 caccatgacg caaatgcgtt gcataaattt acccaccaaa agtgatgcaa atcacataaa
    18781 tcacccttca ttctgcagga ctgcataaaa gatgagccga ttcaggcaat gtgatttgtt
    18841 gcatgctgac tatagttaac attgcgcaac atttttataa gggtacggag aataccatgg
    18901 caacaacaca acaatctgga tttgcacctg ctgcatcgcc tctcgcttcg accatcgttc
    18961 agaccccgga cgacgcgatt gtggcgggct tcacctctat cccttcacaa ggggataaca
    19021 tgcctgctta ccatgccaga ccaaagcaaa gcgatggccc actgccagtg gtcattgtag
    19081 tgcaggaaat ttttggcgtg catgaacata tccgcgacat ttgtcgccgt ctggcgctgg
    19141 aggggtatct ggctatcgca cctgaacttt acttccgcga aggcgatccg aatgattttg
    19201 ccgatatccc cacgttgctt agcggtctgg tagcaaaagt gcctgactcg caggtgctgg
    19261 ccgatctcga tcatgtcgcc agttgggctt cccgcaacgg cggcgatgtt catcgtttaa
    19321 tgatcaccgg attctgctgg ggtggacgta tcacctggct gtatgccgcg cataatccac
    19381 agctaaaagc cgcagtggcg tggtacggca aactgacggg cgacaagtcg ctgaattcac
    19441 cgaaacaacc tgttgatatc gcaaccgatc ttaacgcgcc gattctcggc ttatatggtg
    19501 gtcaggataa cagcattccg caggagagcg ttgaaaccat gcgccaggcg ctgcgggctg
    19561 ctaatgcgaa agcagagatt atcgtgtacc cggacgccgg gcatgcattc aacgctgatt
    19621 atcgcccgag ctatcatgcc gcatctgcag aagatggctg gcagcgtatg ctggaatggt
    19681 ttaagcagta tggtgggaag aagtcgctgt aatgagaaaa gaccgggtgg tattaccacc
    19741 cggtttggat tttacccccg acgcaagttc tgcgccgcct gcaccatgtt cgccagtgcc
    19801 gcgcgggttt ctggccagcc gcgcgttttc aggccacagt ccgggttgac ccacaggcgc
    19861 tctgccggaa tgcgttttgc cgctttcttc agcaaggctt caatccattc cacgctcggt
    19921 acgtttggcg agtgaatgtc atagacgcca ggaccgattt catttggata atcaaactct
    19981 tcaaacgact ccagcaactc catgtcggaa cgcgaggttt cgatggtgat gacgtctgcg
    20041 tccagcgccg caatcgaatc catgatgtcg ttgaactcgc aataacacat gtgagtgtgg
    20101 atttgtgtgt catctttcgc cacggcggcg ttgatacgga aggcctctac gccccactgg
    20161 agatacgcat cccagtcgct acgacgcagc ggtaaacctt cgcgcagcgc cggttcgtca
    20221 atctggatga tgccaattcc agcggcttcc agatcggcca cttcatcacg cagcgccagc
    20281 gcaatctgtt tggcgatggt ttcacggctg acatcttcac gcgggaacga ccagcagagt
    20341 atggtcaccg gccccgtcag catccctttc accggtttgt cggtcagcga ttgcgcatac
    20401 ttcgcccact ccacggtaat cggtgccggg cggctaatgt caccaatgac aatcggtggc
    20461 ttcacgcagc gggaaccgta gctctgtacc caaccgtttt gcgtaaagac aaatccgtcg
    20521 aggtgctcgc caaagtattc caccatgtca ttacgctcgg cctcgccatg taccagcaca
    20581 tccagtccca aacgttcctg ctcaacaatg gcctgcttga tatgttccgc aatgcccgtg
    20641 cggtagttgt tggcgtcgag attgcccttt ttgaaatcca gacgcagggt acgaatttcc
    20701 gtggtttgcg ggaaggaacc aatcgtggtg gtcggccacg ctggcagttt aaaacgcgca
    20761 cgctgggctt cagcacgcac ttcatagaca ttcgcacgct ggctgtcctg ggcggtgatc
    20821 gccgccagac gcttttctac cgccggatta tgtacgcggg tagagtgacg acgtgcctga
    20881 atcggggcgc tccactctgc cagagctgcc gtgtcaccac tgttcagcgc atcgcgcagc
    20941 agtgccagtt catggcattt ttgtagggcg aaggcaaacc agcttttcac ttctgcatca
    21001 agacgcgttt ccacgctcag gtcgatgggg ctgtgcagca acgagcagga agatgccacc
    21061 cacaaatcac gtttgccgac aatgtcctta atttgcgcat atttctcggt aagatcggcg
    21121 cgccagacgt tacggccatt gatcagaccc gcagacagca accagtcaga aggcaggcgc
    21181 ttgtgcagtt cagcaacgtc atctttacca tgtacgaggt caacatgcag accctgaaca
    21241 ggcagcgcag taatcgtgtc gagatttggc gttacgcctt caaaataggt ggtcagcagc
    21301 agtttcacct gtccctggag cgcgtcgtaa gcgggtttgt atgcgtccag ccacgcctgt
    21361 ggtagttcca gtaccagcgc gggttcatca atctgtaccc actcgatgcc gcgtttcgcc
    21421 agttctgcca gcacttgctg ataaaccggc agaatgtcgt tcagcaggct caggcggtca
    21481 aattgttcac ctttcacttt ccccagccac agccaggtaa ccggccccag cagcacaggt
    21541 ttcaccttgt ggcccagcgc cagcgcctcg tccacttcgt ccagcagctg cgtccaggtc
    21601 agtttgaact gttggccttt aacgaactcc ggcaccatgt agtgatagtt ggtgttaaac
    21661 catttggtca tttccgctgc cgccgcaggt tcgccagtcg gcgcacgtcc acgaccaata
    21721 cggaacaggg tgtcgatatc taccgaacca tctttgttct gatgacgcgc cggaacgtta
    21781 cccagcagca gactggtggt cagtacatga tcgtaccagg caaaatcgcc caccggcagc
    21841 aggtcgatac ccgcttgctt ttgttgatcc cagtgacgag cacgcaattc acgccctacc
    21901 gccagcagtt cttcacgcgt ggagttcccc gcccaataac tttcttgcgc ttttttcagc
    21961 tcgcgacgca ggccaacgcg agggaaaccg agggtgtgat tcaatattgt cattttttct
    22021 tcctctaatt atatgtaaat cctatggatt ttgaatttag ggaaggcggc aagtttattc
    22081 atccccggga gcttactgaa gtaagtgacc ggggtggaga aacgcagcca acgcaccata
    22141 aatttaaaag acgaaggatt ttagccgtcc agatgtttac acatccataa ttggcggtta
    22201 ctgtatattc ctcaagcgca aattattcat gccgaagtga aggactttca tgatcgaagt
    22261 aaaacacctg aaaacgctac aagcgttgcg gaactgcggc tcgctcgcag ccgctgcggc
    22321 gacgttgcat cagacgcaat ccgccctgtc tcaccagttt agcgatctgg aacaacgcct
    22381 tggcttccgg ctatttgtgc gtaagagcca gccgctacgc tttacaccgc agggagaaat
    22441 cctgttgcaa ctggcaaacc aggtactgcc gcaaattagc caggccctgc aagcctgcaa
    22501 tgaaccgcag cagacgcgtc tgcgcattgc cattgagtgc catagctgta ttcagtggct
    22561 gacacccgcg ttagaaaatt tccataagaa ctggccgcag gtagagatgg attttaaatc
    22621 gggcgtgaca tttgacccgc agcccgcctt gcaacaggga gagctggatc tggtaatgac
    22681 gtccgatatt ctgccgcgca gtggcctgca ttattcgccg atgttcgact atgaagtgcg
    22741 tctggtgtta gcacctgacc atccactggc ggcgaaaacg cgaattacac cggaagatct
    22801 cgccagcgag acgctattaa tttatccggt gcagcgtagt cgactggatg tctggcggca
    22861 ttttcttcag ccggcaggcg tcagcccgtc actgaaaagc gtcgataaca ccttattgtt
    22921 gattcagatg gttgccgcgc ggatgggtat tgccgcgcta ccgcattggg tagtagagag
    22981 ttttgagcgc cagggtctgg tggtgaccaa aaccctgggc gaaggcttgt ggagccgact
    23041 gtacgccgcc gtgcgcgatg gcgagcagcg tcagccagtt acggaagcgt ttattcgctc
    23101 agcgcgcaat cacgcctgcg atcatctgcc gtttgtgaag agcgcggagc gacccactta
    23161 cgatgcaccc acagtgaggc caggatcacc agcgcgcctg taataaacgt tggccagtgc
    23221 ggctgttggt gccagatagc caggtttacc agcagccctg ccggaacgtg catattattc
    23281 ataatgccca gcgttccggc gtccacctgc gtcgcgccgt agttccacat aaagtagcca
    23341 atcccggaag ccaccacgcc aagaaacacc agaatgcccc attgcagcgt ggtttgcggc
    23401 attttctgcg catttcccag caagaaccat gcaatcactg ccaccagaaa cgcgccaaga
    23461 tagaaccacg caaaggcgtt atgctgtggc atcgggcgag tttccatcag gcgtttgtaa
    23521 cccaccatgc caatggcaaa agtgatattg gagagttgca ccagcagcaa gccagtccag
    23581 aaatggtcgg tgacctgatc atagcgaata atcccggcac caatcaccgc cagcaaggcg
    23641 ctaaaggcat agccccagcg cagacggcgc ttactcatga tgtcataaat cagcgtgatg
    23701 tagagcggcg tcagcacggt gaacagcaac agctcggaaa ccgtcaggta gagataagcg
    23761 cggaaactca gcatatacat cacgccaagc tgcatcgcgc ccaccagcat atacaggccg
    23821 accgttttca ggctattgcc acgggtacgc agaaacggca gaaaaacgag tgccgccagg
    23881 ccaacgcgca ccagcaccgc aaaatagcta tcgacgtgcc ccgcaaggta ctcgccataa
    23941 aagctaaagg agaaggccca cagaatcgtg gtgatgataa gtagcgccac aatggaaaac
    24001 tctttgatta acgggtatcc attgtagcga agtatcaggt tgacaactga ccaaataaag
    24061 aacgattacg ataaatagag tttacgcaga taatgcggca ccgcgtcgtc ggcattagta
    24121 ccaatcactt ccagctcggg atgaaggtct ttcagacgct ggtgcgcact gcccataatg
    24181 cagcctttcc ccgccatcga cagcatttcg gcgtcgttca tcccgtcacc aaacgcaata
    24241 caatccttca ggctgtagcc cagtttcttc gccaccgctt ccagcgcatg gccttttgaa
    24301 acgccgcccg ccatcacttc cagacaggtt aaggtagaga aactgacgtt gacgcgatcg
    24361 ccccaacgag cgttaatcgc ctgctccagc ggcagcagtt gttcatggga atcgcaggtg
    24421 aagaacactt tgctgacgcc ttccggctcc agtaatccag gctcatacag cgcatattgg
    24481 aacaccgctt ctttaaaaaa gcgcatctct tccgggcgat ggcgattcat aaaccattcg
    24541 tcgtcgcgat aaacgttagt aatgatgtcc ggattgtcgt tgactacgcc aaacagatcg
    24601 ctggcaatgt cgcgatccag gttatgagca aaaatcagat taccatccag atcgtgaacg
    24661 cgcgcaccat tggaggtaat catgtaagac ttaatctcca gattatcgcg aatttgcccc
    24721 acatcaacgt ggtgacgacc ggtcgcaaac acaaagttga tgccgcgcgc ggtgagcagc
    24781 ttcagagttt ctttggcgta aggggataac gtatggtcgg gagaaagtaa cgtgccatct
    24841 aaatcagacg caacaacctg gtacataaga aatttaaacc tctgtagagc ggtttccgct
    24901 gggtgagtta tgcctgttga aaaaatcaac gatggcgtgg agcgcgactg agcgcattgc
    24961 gtccttttca aaaaggatct catggtaagc acctttaatt accaacggcc gtcctccttc
    25021 gacaggatgg cccgcggcgg tgcggagttc acaaaaacgg tcatgcatgc ggttatccac
    25081 cacgcgttcc tcttcagcct gcaagagaag cgttggcgtg gcgtcatcac ccgcaccggc
    25141 taacacctgt tcgccagcca gaatactttc gcgtacccaa tggtaggtcg gcccaccgac
    25201 gcgaatcgtt gggtcatcag catagaagcg taagttacgt cgatatcgct gtctgctgtg
    25261 ggtcagtacg ttgatagcaa acggcaacgc gcgccagcgc ccggtgccta ttgcatagcc
    25321 atcacggaaa cgtggatgcg cttcggccca gttgaggatc tgccgtgcca taaatgacgg
    25381 catacgaatc acgatcccaa acattggcgc agttagcgca atggcgtcac atacacctgg
    25441 atggcgttgc agaaataatg tggagatcgc accgcccatc gaatgtgcca gtatatagcg
    25501 tttacgccac ggaccgggct gaacctcctg ctgccagaat gccgccagat catcaacata
    25561 atcattaaag cgattaacat gcccgagatg cggatcggct aacaggcgac cggaacgtcc
    25621 ctgcccgcga tggtcgatga ttaagacatc aaaccccaaa tggaacaggt cataggccag
    25681 ttccgcatat tttacgtagc tctcaatacg ccccgggcag atgactacca cccggtcatg
    25741 gtgctgtgcg cgaaaacgga caaagcgcac cggaatgtca tccacaccag taaactctgc
    25801 ttcatcacgc tgacgccaga aatcagtcag cggtcccatg gtaaaagcag caaacgcgtt
    25861 ttctcttgtt tcccagtctt tttgctgctg aaacatcggg taatctgcct cttaaaccac
    25921 gtaaaatcgt tttttttagc gtgcctgaca caacgctgcg acagtagcgt attgtggcac
    25981 aaaaatagac acaccgggag ttcatcatga ccttagaatg gtggtttgcc tacctgctga
    26041 catcgatcat tttaagcctg tcgccaggct ctggtgcaat caacactatg accacctcgc
    26101 tcaaccacgg ttatcgcggc gcggtggcgt ctattgctgg gcttcagacc ggactggcga
    26161 ttcatattgt gctggttggc gtggggttgg ggacgctatt ttcccgctca gtgattgcgt
    26221 ttgaagtgtt gaagtgggca ggcgcggctt acttgatttg gctgggaatc cagcagtggc
    26281 gcgccgctgg tgcaattgac cttaaatcgc tggcctctac tcaatcgcgt cgacatttgt
    26341 tccagcgcgc agtttttgtg aatctcacca atcccaaaag tattgtgttt ctggcggcgc
    26401 tatttccgca attcatcatg ccgcaacagc cgcaactgat gcagtatatc gtgctcggcg
    26461 tcaccactat tgtggtcgat attattgtga tgatcggtta cgccaccctt gctcaacgga
    26521 ttgctctatg gattaaagga ccaaagcaga tgaaggcgct gaataagatt ttcggctcgt
    26581 tgtttatgct ggtgggagcg ctgttagcat cggcgaggca tgcgtgaaaa ataatgtcgg
    26641 atgcggcgta aacgccttat ccgacttact ctgaagacgc gtctggcatc accgcgaaat
    26701 aatcaaatga atgccaaatc cggcaaataa cgccccggca aaaccatcaa tccacttcgc
    26761 cagacgttga taaccacggc gcatttgcgg cagggcaaac aggctggcaa cgacggtaaa
    26821 ccacgccagc gtttcgacaa tgatcagcgc aaaaatgccc cagcgcgcgg tagtgccaac
    26881 gttatcaccg acaaacaatg agaacaccga gccaaagtag ataatcgctt tcggattagc
    26941 gagattggtc agtaaacctt tcaggaaact gcgcccactt ttcgccagct cgacctgtgg
    27001 cgcaggtgca gaaaccgcct cttttttcag tgcaccacgt agcatctggt aacccatcca
    27061 gcagagatac aggccaccgc ccaccataat cagcgtatgc agccaggcca ttttttcgat
    27121 aatcaaatgc aggccaagca gcgcaatccc agcccaaacc attacgccgc aggtaatgcc
    27181 cagcacgccc atcatcgctt ctttacggga acgactgaca gcggtctgag agacaaaaaa
    27241 gaaatcggga ccggggctca taagcgccac aatgtgcacc atggcgacgg tgagaaataa
    27301 catcaacata catttgactc gcgggggaaa cgtttacgga gtcttcatac tggcactttt
    27361 ttatgctgct gactactctt cgtcatcgcc atcaacatgc gcacgaatca gcgccataaa
    27421 cggtttgcca aagcgttcca gcttgcgcat cccaacgccg ttaacgctga gcatttcgct
    27481 ggcggtgatc ggcatctgtt cagccatctc aatcaaggtt gcgtcgttaa acaccacgta
    27541 cggcgggaca ttactttcat cggctatcga tttacgcagt ttgcgtaatt tggcgaacag
    27601 tttgcgatca tagttgccgc cgaacgattt ctgcatcgct ttcggtttga gcgccacgat
    27661 acgcggcacg gcaagttgca aagaggattc gccgcgcagc accgggcgcg cggcctctgt
    27721 cagttgtagg gcagaatgct gggcaatatt ttgcgtcacc aggccgaggt gaatcagctg
    27781 gcggatcacg ctcacccaat gttcatggct tttatcacgg cccatgccat agactttcag
    27841 tttgtcatga ccatagtcgc ggatacgctg gttattagca ccacgaatca cttccaccac
    27901 ataacccatc ccaaaccgct gattcacacg accaatggtg gaaagggcaa tctgagcatc
    27961 ggttgaaccg tcgtactgtt tcggcggatc gaggcagata tcgcagttcc cgcacggctc
    28021 ctgacgccct tcgccaaaat agttcagcaa taccagacga cggcaagttt gcgcttcggc
    28081 aaacgcgccc atcgcattga gtttgtggcg ctcgatatcc tgcaactgcc cctgcggctt
    28141 ctcttccaga caacggcgca gccacgccat atcagccgga tcgtaaaaca gcatcgcttc
    28201 cgcgggcagg ccatcacgcc cggcgcgtcc ggtttcctga taataggatt cgatattgcg
    28261 cggaatatca aagtggacca cgaagcgaac gtttggttta ttgatgccca tgccgaacgc
    28321 caccgtcgcc accacaattt gcaggtcatc gcgctggaat ttttcctgca catcggcgcg
    28381 aacattattt tccagcccgg catgataggc cgccgcgcta attcccttgc tttgcaggcg
    28441 cgcagcggtg tcttctactt tcgcgcggct gttgcagtag ataatgcctg acttaccgcg
    28501 ctgttcctgc acgtagcgca tcaactgatc gagcggtttg aacttctcca tcagcatgta
    28561 gcgaatattc ggacggtcaa aactgctgat ttgaatcagc ggatcgttca gccccagcag
    28621 gcgcacgata tcctggcgcg tggtgtcgtc ggctgtggcg gtcagcgcca taaacggcag
    28681 cgtcgggaac cgctggcgca actgaccgag cgcggcatat tccgggcgga aatcgtggcc
    28741 ccattgggag atacagtgcg cttcatcaac ggctaataac accggattcc agtgcgccag
    28801 atgctcaaga aagttatcca gcatcaggcg ttccggggcg atataaagca gacgaatttg
    28861 cccggtgcgg cagcctgtca tcacttcaag ttgctgttcg cgggtttgcg tcgagttaag
    28921 gcacgccgcc gccacgccgt tggcttgcag ttgatccacc tgatctttca tcaacgaaat
    28981 cagcggtgaa acaaccacgg taaggccgtt tagcaataag gcagggattt gatagcaaag
    29041 ggattttccg ccaccagtgg gcatgacgac gaggcaatcg cggccggaaa gcacagtgtc
    29101 gataatttct tcctggccgg ggcgaaactg ttggtagcca aaggtttctt gtaaaacctg
    29161 tttagctccg gactccagat tcaacacttc cgcctgcgcc acattcaccc cgattgcctg
    29221 aaataaaaac aggcgctatt ttcagcgcct gcgcgagaaa ctgcaatgct tttagtctct
    29281 gagagggtaa tcctggatgc tggattccag atttgccaac cactcaaccg ttcatcaaaa
    29341 caaatcgttt agcataaccc ccacaccgac acgggtctgg ttgaagttat agtcgatgag
    29401 cgattcgcca tagccgctgt aaacctgagt ataaaggcgc acatgtttgg tgatcgggta
    29461 acttaagcct aactccgcgc cgccgtagcc ggtgttccag ttgtactgtc ctttcgcact
    29521 gagcaccgca tcaccgaggt gatagccgat tttaagctgg tagtaaccca tatatttggt
    29581 gatatccggg ttatcgtcag tattacccac cacataccac ggcttcactt ctaccagcca
    29641 gttaccgttt tctgccatca ggcgagtata aaggcggttc cagctgcggg aggtcgggtc
    29701 ggaacgcccg ttagagtcgt ggttataccc catctccaca tcgcgcagcg tccaacctgc
    29761 aaaacggtaa tcggtggcaa aaccgaggaa caattgcggt tcgtagttgg tttcacgaaa
    29821 cggtgaagac tcttcgctat tggacagttg ccaccaggat ttttgcgtat aagacgcacc
    29881 caacaccgag ttcgggccta aaatcccacg ccacagcgga aatgccaggc tcaactgaaa
    29941 ctttacttca tccttacgcg cattttccgc ccagtcgtaa ctggcaatcg cttctttatt
    30001 cagatcgctg gtttgggtgt aaatgaggta gttggtgtca taaggataga gcgtgaacgg
    30061 attgtcatgc tcctgcagca tattggcgat aatactgcca cgcaccgctg gcgcgtcatg
    30121 cacctctttc accgttgcct cttgtgcata tactgccata ggcaacataa acaccggcaa
    30181 caaccagccc tgcagagtcc gcatcggtcg tgttctccgt aacgaattat tgataaggtg
    30241 aatatttttc tgcgggctat tttacatact taactccata gccgttagtt atcccttcct
    30301 aaagtagaaa ccaacaacaa agaagcataa aatcaacatt tcattaacca atgaagtgta
    30361 aggatgatat gtctgccgta ctgaccgctg aacaagccct gaaattagtg ggtgagatgt
    30421 ttgtttatca catgccattt aaccgcgcat tggggatgga actggagcgt tacgaaaaag
    30481 agttcgcaca gctggccttt aaaaatcagc caatgatggt gggcaactgg gcgcaaagca
    30541 ttttgcacgg cggggtcatt gcgtcggcgc tggatgtcgc cgccggtctg gtgtgcgtgg
    30601 gaagtacctt aacccgccac gaaaccatca gtgaagatga actacgccag cggctatcgc
    30661 ggatggggac cattgatctt cgcgttgatt atctgcgccc aggcaggggc gagcgtttta
    30721 ctgctactag tagcctgttg cgtgcaggca ataaagtcgc cgtcgcccgc gttgaattac
    30781 acaatgaaga acagctttat attgccagtg ccaccgccac ctatatggta ggttgaagac
    30841 ataaaagcag gtaaactgct gttaccaatt aaaaatgagt attcccgatg gatgcaaaac
    30901 aaacgcggca gggcgtatta ctcgctcttg ccgcttattt tatttggggt atagcgccag
    30961 cgtacttcaa gttgatttac tacgtgcccg ccgatgaaat cctgacgcat cgcgtgatct
    31021 ggtcgttttt ctttatggtg gtgctgatga gcatttgccg ccagtggtcc tatttaaaaa
    31081 cgctgattca gacgccacag aaaattttta tgctggcagt ctctgccgtg ctgattggtg
    31141 gcaactggct actgtttatc tgggcggtga acaatcacca tatgctggaa gcgagccttg
    31201 gttactttat taacccgctg gtgaacattg tgctggggat gattttcctc ggcgagcgat
    31261 tccgccggat gcaatggctg gcggtgattc tggcgatatg tggcgtgtta gtccagctgt
    31321 ggacttttgg ttcgctacct attatcgcgc tgggactggc atttagtttt gccttctacg
    31381 gtctggtacg caagaagatt gccgttgaag cgcaaaccgg catgttaatc gaaaccatgt
    31441 ggctgctgcc cgtggcggca atttacctgt ttgctattgc cgacagctca accagccata
    31501 tggggcaaaa cccgatgtcg ctgaatttac tgctgatcgc cgccggtatt gtcactaccg
    31561 taccgctgtt gtgttttacc gccgctgcca cgcgcttgcg tctctcaacg ttaggctttt
    31621 tccagtacat tggcccgacg ctgatgttcc tgctggctgt gacgttttat ggtgaaaaac
    31681 cgggtgccga taagatggtg actttcgcct ttatttgggt ggcgctggca atttttgtga
    31741 tggatgcgat ttatacgcag cgtagaacgt ccaaataaaa aataaatata ttctgaaaaa
    31801 atatgccgac tcttgcatga gagtgggcat attgccatgc tgaccggaga ctcatacaag
    31861 gatgtaaaat ttatgttacg tatatttatc ccaacatcta acggtaagat ttccagacgt
    31921 cgttatatat tttcatttat tttaataaat ttcatttttg cctttcttat tatctttttt
    31981 aacgatggag aagcaggctt tcttgttata gtctctacca tcgtactcca ttatctcgtc
    32041 atcaacatga attgccagcg actccgtgat agtggtttca tctatataaa aacttatgtt
    32101 tttggtacat tagcagtata tatcatctct atcatcacaa tgatagccga agattttgcc
    32161 tgttcaggca atggttcgat gatttttttg atttgttatt tttccacttt cagtatgcta
    32221 atgcttgcac ctactgattc atcaaaacaa tagttatgga gtatttatga gtaaggaata
    32281 tatgaacgat ggttcattaa gtgaaaaatg gaaataccga tttaattttt atgatcaaca
    32341 tggttttcct ggattctggg gggcaacacc agaatataaa gcagcattta aagcattaaa
    32401 agtaagacaa cgcttaacta ttcaaatgaa tttcattgct ttcttctgtt cctggatata
    32461 tttatttgtc ctggggttat ggaaaaaagc aattatagtt ctattacttg gtattctatc
    32521 tctatttgtt ggtgcactaa ttggcgttaa cattctggga attgccgtag cagcctatgt
    32581 tgctgttaac acgaacaaat ggttctatga gaaagaggta aaagggttaa atacctggag
    32641 cctttgaata acaggtggct tagccagact aagccaccgc tctcgttttt tacaaccagt
    32701 tcttccgctt aaagtacaga tacggtgcca ggcccgcgag gatcataaag ataatcgcgc
    32761 cagggtagcc gaagctccac ttcagttctg gcataaactc aaagttcatg ccatagctgg
    32821 aagcaacgag cgtcggcggc aggaatacca cggataccac cgagaagatt ttgatgatgc
    32881 ggttctgctc gatgttgata aaacccattg ccgcctgcat caggaagttc accttctgga
    32941 acagggattc gttatgcggc agcagggatt cgatatctcg caggatttca cgcgcctgct
    33001 ccagttgccc acccggtaaa cgcgctttac gcaccaggaa gttgagcgcg cgctgggtat
    33061 ccatcagaca caggcgaact ttccagccga tatcttccag ttccgccaga gtggagagcg
    33121 cctcgtcgta ctcatcgccc tgatgccctt ccataatcac ccggctcaac tgctccaggt
    33181 cgctatagat attttcaatt tcatctgcca actgttcgat tttggtttcg aacagatcca
    33241 gcagcaactc gtaggcgtta ccgtctacca tcgactggct acgggcacgc atacgataca
    33301 gacgaaaagc gggcagttca cgctcacgca gagtaaacag acgaccatca cggatggtaa
    33361 atgccacagt ggagttaccg gcgtgatctt ccgcatcttc aaagaagaag aaggagtgaa
    33421 tatgcaggcc gtcgtcgtct tcaaagaaac gtgccgatgc ttcgatgtct tccagttccg
    33481 ggcgggttgc caggctctgg ccaagttcag attgtacgcg cagtcgctcg tcgtcgtccg
    33541 gttcgacaag atcaatccat actgcattta caaggggttg tgactcttcg acttccagcc
    33601 gggtcagtcg gttattttcc agttgaaatg cgctcagcat gaccgggact cccaatgctt
    33661 aaaatatcgg acagttcggt gggcacacag aaacaaattg ggtttcagac cattaaacag
    33721 cctgactcag cgcgacggga aaaattgagg tcgctgacaa ccgctaaggc tatcagcaaa
    33781 aagggatagc cttaggagtt gatcctggat gacaggatag tgagccagta tctactgggt
    33841 gtgtccaagg cgaatgtcct cttagagtga tcgtgcgcgc atgttacgcc agcaaaattt
    33901 tgccgtcaac acgcaacaga acaccacaga acaatagttg aacttcgcgc tttttggtac
    33961 tcgtcaaggt cgctaacagg ataaaaaaat cccacatcca ggacctataa tggcgcatca
    34021 gctactcatt ggtaaaggaa tgatcacctt gaatctcaaa cggatttttc tcgccctcac
    34081 cttacttccc ttgtttgctg ttgccgctga tgattgcgca ctctcagatc cgacgctgac
    34141 cgtacaggcg tataccgtta atcctcaaac agagcgggtg aaaatgtact ggcaaaaagc
    34201 caatggcgaa gcgtggggaa cgttacatgc tctgctggcg gatattaata gtcagggtca
    34261 ggtgcagatg gcgatgaacg gcggcatcta tgatgaaagc tatgcgccgc tcggtttgta
    34321 catcgaaaac ggtcagcaga aggtggcgtt aaatctcgct tcaggtgaag ggaatttctt
    34381 tatccgtcct ggcggcgtgt tttatgtcgc gggagataaa gtcggcatcg ttcgtctgga
    34441 tgccttcaaa accagtaaag agattcagtt tgcggtgcag tcagggccaa tgttgatgga
    34501 aaacggtgta attaatccgc gtattcatcc caacgtcgcc tcaagcaaaa ttcgtaacgg
    34561 tgttgggatt aataaacatg ggaacgccgt gtttttgttg agccagcagg caacaaattt
    34621 ttatgatttt gcctgttatg ccaaagcgaa actgaacgtt gagcagctgc tttatcttga
    34681 tggcacgatt tcacatatgt atatgaaagg cggggcaatc ccgtggcaac gctatccttt
    34741 tgtcaccatg atttccgtcg agcggaaagg ttaaaacgat agttttgtgt cagatactga
    34801 agatggcgca gatgccggat gcggcgtaaa cgccctatcc ggcctacatg acgttgcaat
    34861 ttattgaatc tgaatgattt tttaggccaa ataaggtgcg cagcaccgca tccggcaacg
    34921 ttacaccgac tccagccggg cgtatgccgc caccagccat ttaataccct ggccctgaaa
    34981 tgccacctgc aaacggctat gctcaccgct gccttccata ttgacaatgg tgccttcacc
    35041 aaacttagcg tggcgtacgc gctggccgag cttgtagccg ctgtcgttct cgaccatcgg
    35101 cgtacccatc cgctgatggc tgaccgggcg gcttaccgtg gcgcgcaggc gcacctcttc
    35161 cacacactct tccggcagct cgccgataaa gcgcgacggg cgatggtaaa cctctttacc
    35221 atacagacgg cgggtttccg cgtaggtcag cgtcagtttc tgcatcgcgc gggttacgcc
    35281 aacgtaggcc agacggcgtt cttcttccag acgcccgcct tcatccagcg acatctggct
    35341 tgggaacatg ccctcttcca taccaacgat aaacacctgc gggaactcca ggcctttcgc
    35401 cgagtgtagc gtcatcaact gcaccgcatc ctgccaggta tccgcctgcc cttcacctgc
    35461 ttccagtgcc gcatgggaga ggaacgcctg cagcggcatt aaatcttcgt cttcttcgtt
    35521 gtagctgaac tggcgcgttg ccgtcaccag ttcctctaag ttttcgatac gcgtctgacc
    35581 tttttcgccc ttctcctgct catacatggt acgcaggccg gagtctttaa ttacccggtc
    35641 agtctgtaca tgcagcggca tatcggcagt ttcctgcgct aaggcgtcga ttaattccat
    35701 aaatcgctgc aaggcgctgg cagctcgccc ggcgagggct ttttcctgca acagctcacg
    35761 acatgcctgc cagagtgtta actggcgatc gcgcgatgta tgacgtacca cgtccagcgt
    35821 ccggtcacca ataccccgcg ttggcgtatt caccacacgc tcaaaggccg cgtcgtcgtt
    35881 gcggttggca atcaggcgca gatacgagag cgcatctttg atttcctggc gttcgaagaa
    35941 gcgcatcccg ccgtaaatac ggtacggcat actggcctgc aataacgcct cttcgagcac
    36001 ccgcgactgg gcgttgctgc ggtagagaat ggcgcactcg gcaagcgctc cgccgttgtc
    36061 ctgccaggtt ttgatgcggt taaccacaaa acgcgcttca tcgagttcgt taaaagcgca
    36121 atagagggaa ataggctcac cgtccgcgcc atcggtccac agttttttac ccagacgccc
    36181 gttattgttt tcaatcaggg cgttagcggc gctcagaata ttgctggtag agcggtagtt
    36241 ttgctccaga cgaatagttt cggcaccggg gaaatcatta aggaaacgct gaatattctc
    36301 cacctgcgcc ccgcgccagc cgtagattga ctggtcgtca tcaccgacga tcatcacttt
    36361 gccggtgtcg cccgccagca ggcggatcca cgcgtactga atgttgttgg tatcctggaa
    36421 ttcgtccacc aggatattgg taaaacgttc gcggtagtgt tgcaggatat gcggcttgtt
    36481 aagccacaac tcgtgagcgc gcagcagcag ctcggcgaag tccaccaggc ccgcgcggtc
    36541 acacgcttcc tgatacgcct gatacacctt ctgccaggtc tgctccaccg gattaccgta
    36601 gctttgaata tgatgcggac gcaggccttc atctttctgg ctgttgatgt accacattgc
    36661 ctgccgcggc ggccactgct tctcgtcgag gttcatggct ttgatcagac gcttaagcag
    36721 gcgtagctgg tcttcactgt cgaggatctg gaaatcctgc ggcagattgg cgtccatatg
    36781 gtgcgcacgc agcaaacggt gcgccagccc gtggaaggtg ccgacccaca taccgccctg
    36841 gctcgtgccc atcagttgcc cgatacgatg acgcatctcc gccgccgctt tgttggtaaa
    36901 cgtcaccgcc ataatcgagt atggcgagca gttttccacg ctcatcaacc aggcgatacg
    36961 atgcaccagt acgcgcgtct taccactgcc cgcgcccgcc agcaccagaa ggttgctgcg
    37021 tggcgcggcc accgcttcgc gctgtttgtc attaaggctg tcgagcaggt aagaaacgtc
    37081 cattggcacc gccgcgtaaa aataggttgg gcggagaagc gcaacgccct ccgccaaaaa
    37141 gctgggtata tatacagatt tgctgattat atcagcgagg tcagagatgc caaccgggaa
    37201 atttccagat gcggcagtaa acggctgtcc caggtttgca tcagatcgcc attttccggt
    37261 ctgatccaac aagcctgcat tccgctgcga attgccccac ccacgtcagt ggtgagatcg
    37321 tccccaacat gtaagatctc gccgatcggc acgttgagtt tttccgcagc caaaaagtac
    37381 atatcgctga acggttttga gcgcccgtgc gggccagcgc gcagcacaaa ctcaaaataa
    37441 tcccccaaac caaacagctc cggctgggcg ttaccgttgg tgatcgccac cagcggccat
    37501 ttcttcgcca gctgttttaa ggtgtcgtga gtttgctgcg ggacgtcgat tcggctgcgc
    37561 catttggcaa agttgatcat tgctgcgtgt gcgcctgcac tggcttcttc ggcactcagc
    37621 ccggcgtcga gcatcgcttg ttcaatcgaa cgaaaacgcc agcgcgtcac gtcgtgataa
    37681 atctcgggtt ccgcttcccg taccgcctgg cgcaggcgtt gcagatcttc attctggaag
    37741 ctgcgcagcg ccggatgata attttgcaca aaggtaagcg cctctcgctc ggtgcgcaaa
    37801 atcaccggac ggttatcgta aagggtatca tccaggtcaa aggtgagcgc cgagatgcgc
    37861 cccaaaggcc ggtaaaaacg cattatttcc cccgtttggc gcgtggatgc gccgcatcgt
    37921 acaccgaggc aaggtgttga aaatcaagat gagtatagat ttgcgtggtg gagaggttgg
    37981 catgacccag cagctcctgc acaccacgaa gatcgccgct cgactccagc atatgcgtgg
    38041 cgaacgagtg acgtaattta tgcggatgaa cgtgattatt cagcccttgt tttatgcccc
    38101 attcggcaaa gcgtttctgc acattacgcg cggagatacg cttgcccagt ttcgacagaa
    38161 aaagcgcgtc gtcttcgcta ccaaacaggt cgcgcaaatc aagccagtgc tcaatccacg
    38221 ccacagcgtt gcgaccaatc ggcaggcggc gctctttgct gcctttcccc ataacccaca
    38281 cttcaccaga ctccaggtcg aggtgtttaa tatccagccc caccagctca gaaagacgca
    38341 gacccgcgcc gtacatcact tccagcattg cacggtcgcg tacagcgagg ggatcattga
    38401 tatcaatatc cagcagccga ttcatatcgt cgacgtcgat gtttttcggc agatgacgcg
    38461 gcgctttcgg tgccgaaaca cctttcgccg ggttagcttt gagttcgttc tggctgacca
    38521 gccagtcaaa aaagctacgt agcgcagaaa gccgtaacgc cagacttgct gctcccagcc
    38581 ctttacggcg actgcgtaca gcaaaattgc gcaccatcgt cacatcacat tgctgccagc
    38641 tttgcaggcc gttttcgctg gcaaaattga tgatcgcctc aagctgacgc tggtagttaa
    38701 gcagggttat cgggctaagc tggcgctcca cgctcagata acgtaggtag cgttctacat
    38761 cggtgtgtaa atcggtcata cgcgttcaat ccaacgctcc agaagctccg gcaacatcag
    38821 cgcaatttca tgaagtaact gcgttccttg cccttgttga tagtgactgg catcgcgact
    38881 ggtaaacagc acgacaccca aatcagcatc gcttcccagc atcgacatcg ccaccgatcc
    38941 caccgctttc gcttccggta gcaccaccag cagctctggt ccgttaagcg gcccaagata
    39001 gtgctgttcc tgccccaaac gctgaatacg cagcggttcg aaagactgac ggcttaatgc
    39061 cagatgagtg tggttcgacg gcgcacctaa gcgccagcga tccggaaaca ggcgcagact
    39121 cgcacctgcc aggccgagat cgcgcgccca gcggtgaaag cgcatcagca tatcgtcgag
    39181 actgctggcg gcggtgagac tgcgctgcag gtagagtagg cgataaaaca ggccttcgtt
    39241 ggcgatagcc tgttccatca acagcgccat gttctcttcc agaacatgaa tatgattacg
    39301 tgcgcgggcc atgtgccact cgaccaacga aacggtgccg cgtaccggat gcggcacacg
    39361 tatcgcttct actgcgcgcg cattacggat aaaaaactca ggatttttaa tcagataatc
    39421 gacaaccgcc cggtcatcaa gctccgtgag tgtttcctgc agttcttccc ctggttgctt
    39481 catagatgaa taaatccgtc gtagacatgt accgccgggc cagtcatata taacgggtga
    39541 cccggacctt tccaggcgat atcaagacga ccgccgggga gttccacgcg tacttcttcg
    39601 gccagcaaac cttgctgaat ccctactgca accgccgcac acgcgccgct gccgcaggcc
    39661 tgggtttctc ctgccccacg ctcataaacg cgtaaacgaa tatgctcgcg cttaaccact
    39721 tgcataaaac cgatattggc gcgctccgga aaacgctcgt ggctttccag aacaggacca
    39781 agcgtttcta ccgccgcggt atcgacatca tcgacctgaa tcacgcaatg cggatttccc
    39841 atcgacacca cgccgcataa gattgtctgc tcggcggcgc gcataatata ggtcttttcc
    39901 gctttgttag cgcgaaacgg cacggcggaa ggttcgaagt tgggttcgcc catatttacg
    39961 cggaccagat catcatcggt gacggtcaga accatccgcc cgttggcggt gctgacgcgg
    40021 atatcacgct tattggtcag tcctttcaga cgcacaaaac gggcaaagca gcgcgcaccg
    40081 ttgccgcact gcgccacttc actgccatca gcattgaaaa tgcgatagtg aaaatccagt
    40141 tcaggatcat acggcggctc aaccaccagc agttggtcaa accctacccc caggtgccga
    40201 tcagccaggc gacgaatcag ctccggtgaa aaaaagacat tctgcgttac cgcgtcgacg
    40261 accataaaat cgttgccaag gccatgcatt ttcgagaact gcatcattta ctccaatcac
    40321 gcgggtacag aaactgactt ttagtaattc acctgggatg gaccatcgcc agtggcgcga
    40381 tcgtttttat ccggcaccgt ggattgcgtt tgcgtctcta ccggtttggt cggcggcggt
    40441 gcgtttttat ctgcaggcgg gaaatagagc ggacctttca gaccgcagcc cgtcaggctg
    40501 aagagagtaa gtaatacagt gagtgcctta aacacgtttt tcattatgga ttgcctgtaa
    40561 agttcatcgc tttctgcttt ctatcatcgc aggagaagac gtaaaagcaa gcgtttagcg
    40621 actctttatg aagtctgaaa agtgaggaaa tgctgcgagt ataacgctta ctcgtgggga
    40681 tttatgtttt gctggtagtt tcgtgatcgg cacctcgcgc caggatggct ttatgcacta
    40741 tcgcaatgtg gtttgtttgt tgtctttttt agttccgtca gcgtgaggat gctcgctcgg
    40801 taaacctgat cataatgttt accagaaaca gggaaagacg tggtgtaact gcgccctaat
    40861 gaagagacaa atctttcgct acacagagac atcattattc cggtgacgga tgaatcgtgc
    40921 cggaacgcag ccgcagaggt gcaaactttg aagccctgac cccatgccgc tatactgccg
    40981 ccatcactta aaaacaggat acaacaatga acgacagtga atttcatcgc ctggctgatc
    41041 aactctggct gaccattgaa gaacgcctgg acgactggga tggcgacagc gatatcgact
    41101 gcgaaatcaa cggcggcgta ctgaccatta cctttgagaa tggcagcaaa atcattatca
    41161 accgccagga gccgctgcac caggtatggc tggcaaccaa acagggcggc taccattttg
    41221 acctgaaagg cgatgagtgg atttgtgatc gcagcggcga aaccttctgg gatttgctgg
    41281 aacaggcggc gacgcagcag gcgggtgaaa cagtcagttt ccgctaagat tgcatgccgg
    41341 ataagcctcg ctttccggca cgttcatcac gaaaaatatt gctgtaatag cggcgtatcg
    41401 tgatcctgat tggcaggcgg catgttaccg atagattttg tgcggaacgg aatcacctgt
    41461 tcacgaccat caaccttcac aatctgatag aactgcggca ggttgaagtt gatgaagctt
    41521 gagccgtagg taaaacggtc atgcgatgac gagtagaagc gactgacgtc acgtaccagc
    41581 tcctctttgc tgccttcgca gtggtgatat acctcaaccc ggttgctttc gtcgagaatg
    41641 tagatattaa agccattctc gtcttgcgtt tcttcgaaaa agaactggat gatcccttcg
    41701 ctggcaaagc cgtccaccac cgccggtaat ttgacgtgat tggtttcaac ctgcactgac
    41761 aggccgtgca gtttgttatg cgaaatcgcg ccataaaact cgatggcgtt ttccagtttc
    41821 tgtaccgata cattcaggcg ttcgaagaac aacccccagg tttgaccaga aacgcgcagc
    41881 gccttgaaac gcccggtttc ctggcgggtg ctggaaagac gcaattcaat acactcagaa
    41941 accagttgct gcacgcgagt acgaattaag ccgcgcagat gctggctata acagaagact
    42001 tccacgctat ctggcggtgc ggcgtcctga tgcattttgc cgagaatagt tttcagggct
    42061 tcgatcatcg attgctcgcc gttgaagtgc agcgtacgca cttcgttcca cgagttgcgg
    42121 tacagcaggt caacgctacc taccaggcaa ttttgattct cgccaaagct gaagacatcc
    42181 agcttacgga aatcgaaatg caccacctga ttgcggaacg ccgctgtcgg gtcatattcc
    42241 aggttaacga taatcgccag atggcggatc tcacacgggc tgtagagcgc cttcggtgtc
    42301 ggtgcaggta agcgcagcgg gaaatggtgc gacacgtcgg cgaccatctc ctgcaactta
    42361 ggcaaatcga caatgccgtt acctttaata tacaaacggg tgcgcgaggt cagcaggccg
    42421 ttaaaccatg cccacgccac cagtttattc aggtaacggt tatattccag cggctgatgg
    42481 ctgatgatcg actcaatatt tggcgcgcgg ttatacagat accaacctga acggttagcc
    42541 cggcccggcg gcacataaat aaaggtcaga ttcggttccg agagatcggg tgaaatctgc
    42601 gggtttacca gcgtcacttt acctggtaat gcttcaaacg cggcatacag cttacgcgtc
    42661 agcacgccga tatcctgcgg actggcggag acgctaaggt tattgcgacg cgcaaagcgg
    42721 atcagattac ggtagctctg catcatcgcg tcgagcaact cgttgtgcgc ctcacgcacc
    42781 tgatcaatct tccagttagc gcggttatcg agcattgcca gacgagcttc gtcccaaccc
    42841 cactcgctca ctaactggct caacactgcg cgacgccagc ctacgcaggc gcgttcacgg
    42901 ctgagctttt cgcacacttt taaatagaag cagcgacgta ctaaatccag acgggtaaga
    42961 tcttcaatcg ccgtcaggta ttcagtaaca cgctccagca tcatgcagta tggatcgaga
    43021 ccaaacgata caatctcgcc gtcgtgcaaa cgctgtttga tatctttcgc cagcagacgt
    43081 gggttcgggt attcccagga ataggcttcc agcagcagtg ttttcagtac cgctttgtat
    43141 ggggaatcga tactcttgta gagctgccaa aggctggcac caaagtactc ttcagcagaa
    43201 agcgagctta agccaccgag atccagccat tcatttggcg tcagcacgcc ctgcgcgtaa
    43261 agcgtcatca catagtcgtc gtaatgctct tcttcgtcgc acggcaccat attccacaga
    43321 atacgcttac cggcgagacg cacggcggta cgataaaatt cgtcaagcag cagtatatgc
    43381 tgggtggagc cacaatcttc gccccccagg ctgccgcttt cattatgacg gaagcggttt
    43441 tcatcaatca ggaagaagct gacttccaca cccagcgagg cggcccagtt ttccagcagg
    43501 ctacatttac gttgtagcaa ttggcgctct tcgctatcga gccaggattg atgacagacc
    43561 cagatatcca ggtcagagga acaactttgc cctacggacg aggtgctgcc catggtgtat
    43621 acaccagtaa ttggaagctc acctttcggc ggatcctgta ctgacattcc acgatacagt
    43681 tcaagctcgt tcaggtagtg gcgttgagtt tcatcaggcg tgtaaaggca aatgcctttg
    43741 ggaacgttac catcaaggta acccggcatt agcggatggt gatagtgcaa caatgtcggc
    43801 agtagactgt agacctgttg gaatgcaggc cccatagcag caagcgcgcg atccacacgc
    43861 aattgattta tggcatccag tctctgtttc agagtctcaa tatagaggta caagacgtat
    43921 cgcctgattt gctacccgtc atgactgtga ttccgccaac atcaacggta acacgcggca
    43981 ttcgggatat ttcgtatgtc aaaggtaacc gttaccactt ttcgcgcctg gtttttttag
    44041 tttcacgacg aaaaaatggt ctaaaacgtg atcaatttaa caccttgctg attgaccgta
    44101 aagaaagatg cgctacatac aagtgtagca ccgtttattc tctgtaaatt ccttattaca
    44161 acggcgtgaa acgcctgtca ggatccactg ccagacctca ttttacggtt tgcgcaggcg
    44221 tctacgtttc accacaacac tgacatcact ctggcaagga tgttaggatg gaccacggat
    44281 gataatgacg gtaacaagca tgttagacaa tgttttaaga attgccacac gccaaagccc
    44341 acttgcactc tggcaggcac actatgtcaa agacaagttg atggcgagcc atccgggcct
    44401 ggtcgttgaa ctggtaccga tggtgacgcg cggcgatgtg attcttgata cgccgctggc
    44461 gaaagtaggc ggaaaaggct tatttgtaaa agagctggaa gtcgcgctcc tcgaaaatcg
    44521 cgccgatatc gccgtacact caatgaaaga tgtgccggtt gaattcccgc aaggtctggg
    44581 actggtcact atttgtgagc gtgaagatcc tcgcgatgcc tttgtgtcca ataactatga
    44641 cagtctggat gcgttaccgg caggcagtat cgtcgggacg tccagtttac gtcgccagtg
    44701 ccaactggct gaacgccgtc cggatctgat tatccgctcc ctgcgcggca acgtcggcac
    44761 tcgcctgagc aaactggata acggcgaata cgatgccatc attcttgccg tagccggact
    44821 aaaacgttta ggtctggagt cacgtattcg cgccgcgttg ccacccgaga tttctcttcc
    44881 ggcggtagga caaggtgcgg tgggtattga atgccgcctt gatgattcac gcactcgcga
    44941 gctgcttgcc gcgctgaatc accacgaaac tgcactgcgc gttaccgcag aacgcgccat
    45001 gaatacccgt ctcgaaggcg gatgtcaggt gccaattggt agctacgccg agcttattga
    45061 tggcgaaatc tggctgcgtg cgctggtcgg cgcgccggac ggttcgcaga ttattcgcgg
    45121 tgaacgccgc ggtgcgccgc aagatgccga acaaatgggg atttcgctgg cagaagagct
    45181 actgaataac ggcgcgcgcg agatcctcgc tgaagtctat aacggagacg ccccggcatg
    45241 agtatccttg tcacccgccc gtctcccgct ggagaagagt tagtgagccg tctgcgcaca
    45301 ctggggcagg tggcctggca ttttccgctg attgagtttt ctccgggtca acaattaccg
    45361 caacttgctg atcaactggc agcgctgggg gagagcgatc tgttgtttgc cctctcgcaa
    45421 cacgcggttg cttttgccca atcacagctg catcagcaag atcgtaaatg gccccgacta
    45481 cctgattatt tcgccattgg acgcaccacc gcactggcac tacataccgt aagcggacag
    45541 aagattctct acccgcagga tcgggaaatc agcgaagtct tgctacaatt acctgaatta
    45601 caaaatattg cgggcaaacg tgcgctgata ttacgtggca atggtggtcg tgagctaatt
    45661 ggggataccc tgacggcgcg cggtgctgag gtcacttttt gtgaatgtta tcaacgatgc
    45721 gcaatccatt acgatggtgc agaagaagcg atgcgctggc aagcccgcga ggtgacgatg
    45781 gtcgttgtta ccagcggtga aatgttgcag caactctggt cgctgatccc acaatggtat
    45841 cgtgagcact ggttactaca ctgtcgacta ttggtcgtca gtgagcgttt ggcgaaactc
    45901 gcccgggaac tgggctggca agacattaag gtcgccgata acgctgacaa cgatgcgctt
    45961 ttacgggcat tacaataact ctcataacag gaagccataa tgacggaaca agaaaaaacc
    46021 tccgccgtgg ttgaagagac cagggaggcc gtggacacca cgtcacaacc tgtcgcaaca
    46081 gaaaaaaaga gtaagaacaa taccgcattg attctcagcg cggtggctat cgctattgct
    46141 ctggcggcgg gcatcggttt gtatggctgg ggtaaacaac aggccgtcaa tcagaccgcc
    46201 accagcgatg ccctggctaa ccaactgacg gcattgcaaa aagcccagga gagccaaaaa
    46261 gccgagctgg aaggcattat taagcaacaa gctgcacaac ttaagcaggc gaatcgtcag
    46321 caagaaacgc tggcaaaaca gttggatgaa gtccaacaaa aggtcgccac catttccggc
    46381 agcgatgcta aaacctggct gctggctcag gccgattttc tggtgaaact cgccggacgg
    46441 aagctgtgga gcgatcagga cgtcacgacc gctgcagcgt tgctgaaaag tgcagacgcc
    46501 agcctggcgg atatgaatga cccgagtctg attaccgttc gtcgggcaat taccgatgat
    46561 atcgccagcc tttctgcagt atcgcaggtg gattatgacg gcatcatcct taagcttaat
    46621 cagctttcaa atcaggtaga taacctgcgt ctggccgata atgacagcga tggttcgccg
    46681 atggattcag acggtgaaga gctttccagt tccatcagcg aatggcgtat caatctgcaa
    46741 aaaagctggc agaactttat ggacaacttc attacgattc gccgtcgtga tgacaccgcc
    46801 gtaccgctgt tagcgccaaa tcaggatatc tatctgcgcg aaaatattcg ctctcgcctg
    46861 ctggtcgcag cacaagctgt accgcgtcac caggaagaga cttatcgcca ggcgctggag
    46921 aacgtctcca cctgggtacg tgcttactac gatactgatg atgccaccac caaagcgttc
    46981 ctcgacgagg tggaccagtt aagccagcaa aatatctcga tggatcttcc ggaaaccctg
    47041 caaagccagg cgatgctgga aaaactgatg cagactcgcg tgcgtaacct gctggcacaa
    47101 ccggcagcgg ggacaacgga agctaaacct gcacctgcac cgcaagctga tactccggca
    47161 gccgcgccgc aaggagaata accatgctaa aagtgttatt gctctttgtg ttgctgattg
    47221 cggggatcgt ggttggcccg atgattgccg gccatcaggg ttatgtgctg atccagaccg
    47281 acaactacaa tatcgaaacc agcgtcacgg gcctggcgat catattgatt ctggcgatgg
    47341 tagtgctgtt tgccattgag tggctactgc ggcggatctt ccacactggc gcgcacaccc
    47401 gtgggtggtt tgtcggacgt aagcgtcgcc gtgcacgtaa gcagaccgaa caggcgctgc
    47461 tgaaactggc ggaaggcgat tatcagcaag ttgaaaagct gatggcgaaa aatgccgatc
    47521 acgcggaaca accggtggtg aactatctac tggctgccga agccgcgcaa caacgtggtg
    47581 atgaagcacg cgccaaccaa catctggaac gcgcagcgga gctggccggc aacgacacca
    47641 ttccggtaga aatcacccgc gtacgtctgc aactggcccg taatgaaaac catgctgcac
    47701 gccacggcgt ggataagctg ctggaagtta cgccacgcca tccggaagta ttacgtctgg
    47761 cggaacaggc gtatatccgc acaggtgcat ggagttcgct gctggatatt atcccatcaa
    47821 tggcgaaagc ccatgttggt gatgaagaac atcgtgcaat gctggaacaa caggcatgga
    47881 ttggcctgat ggatcaggcg cgtgccgata acggtagcga aggtttgcgt aactggtgga
    47941 aaaaccaaag ccggaaaacg cgtcatcagg tagcgttgca ggtggcaatg gcggaacatc
    48001 ttattgaatg tgacgatcat gatactgccc agcaaattat catcgatggc ctgaaacgcc
    48061 agtacgacga tcgcctactg ctgccgattc ctcgactgaa aacaaacaat ccggaacagc
    48121 tggaaaaagt gctgcgccag caaatcaaaa acgtcggcga tcgcccgctg ttgtggagca
    48181 cactgggcca gtcactgatg aagcacggag aatggcagga agcatcgctc gccttccgcg
    48241 cagcgctgaa acaacgtccg gacgcctacg attacgcatg gcttgccgac gcgctggaca
    48301 gactgcacaa gccggaagaa gctgcagcga tgcgtcgcga cggtttgatg ttaacgttgc
    48361 agaataaccc gccacagtag ttccttctca cccggaggca agcacctccg gggccttcct
    48421 gatacataaa aaaacgcctg ctcttattac ggagcaggcg ttaaaacagg tctgtatgac
    48481 aacaagtggg tgcttcactc aacgttgtgt ccatggtgtc tgatgaggca taagcgacat
    48541 ctgtcagtgg acgataagca ccgtaaacgg ctctgcgtca ttccggagtt tatgaggcac
    48601 taaggcgaac ataagagatg gaatgagcat ctactcgttt attatgccac agagaatcgg
    48661 gaaataacat cccttaacac ttgttatgag ataattctgt aatcctcttt gcttcctgag
    48721 taataacttc ctgagtgaat atttaacctg agcttgatcc tacacatact tattatgaat
    48781 gataaaattc attcaattaa taacacatat attaattgcc gttaaaacta aaaacagcat
    48841 caataatcaa cgcgatataa taaacctgcc ttacatatca actgcgccag aggtaggatt
    48901 gaaaacgctc tcctgatttt ccaattcatt ttctggataa ataaataatt tatttttgtc
    48961 actattattt atgtaatcat cctgtcaggg agagggatct caattatcaa tgcttaatta
    49021 cgtcatcatt ttgatgacat gaaaccggag cattttgcat ggaattttcg ttttcaccca
    49081 aacgtcttgt tgttgctgtc gccgccgctc ttcctctcat ggccagcgca gcagataccc
    49141 cgtcaactgc caccgcacgc aaaggctttg ccggatacga tcacccaaac cagtatctgg
    49201 ttaaaccggc gaccactatt gccgacaata tgatgccagt aatgcagcat ccggcgcagg
    49261 ataaagaaac ccagcagaag ctggcagaac ttgagaaaaa aaccggtaag aaaccgaatg
    49321 tggttgtttt cttgctggac gatgtgggct ggatggacgt cggttttaac ggtggcggcg
    49381 tggcggtggg taaccctaca ccagatatcg acgccgttgc cagccagggg ctgattttaa
    49441 cttcggcgta ttctcaacca agctcttccc caacccgcgc caccattctc accggacaat
    49501 actccatcca ccacggcatt ctgatgccgc caatgtacgg gcaaccgggc gggctgcaag
    49561 ggttaaccac gctgccgcag ttgctgcacg atcagggcta cgtcactcag gccatcggaa
    49621 aatggcatat gggggaaaac aaagagtcgc agccgcagaa cgttggcttt gatgatttcc
    49681 gtggctttaa ctcggtgtct gatatgtaca ccgaatggcg cgacgttcac gtcaatccgg
    49741 aagtggccct gagtccggac cgttctgaat acatcaagca attaccgttc agcaaagatg
    49801 acgttcatgc ggtgcgcggc ggcgaacaac aggccattgc cgacattacg ccgaaatata
    49861 tggaagatct ggatcaacgc tggatggact atggcgttaa gttcctcgac aagatggcga
    49921 agagcgataa accattcttc ctctactacg gcactcgtgg ctgccacttc gataactacc
    49981 caaatgcgaa atatgcgggt agctctccgg cacgcacctc gtatggcgac tgcatggtgg
    50041 agatgaacga tgtgttcgct aatctgtata aaacactgga gaaaaacggt cagcttgata
    50101 acacgctgat cgtctttacc tccgataacg gaccggaagc cgaagtaccg ccgcacggac
    50161 gcaccccgtt ccgtggtgcg aaaggttcga cctgggaagg cggcgttcgc gtaccgactt
    50221 tcgtttactg gaaagggatg atccaaccgc gtaaatctga cggtattgtc gatctggcag
    50281 atctcttccc taccgcgctg gatctggcag ggcatcctgg agcgaaagtg gcgaatttag
    50341 tgccgaaaac cacctttatc gatggtgtgg accagacatc cttcttcctg ggaacaaatg
    50401 gtcagtctaa ccgtaaggcc gagcactact tcctcaacgg taaactcgct gctgtgcgta
    50461 tggatgagtt caagtatcac gtcctgattc agcaacctta cgcttatacc cagagcggat
    50521 atcagggtgg attcaccggc acagtaatgc aaacggcggg atcgtcggtg tttaacctct
    50581 acaccgatcc gcaggaaagc gactccatcg gcgtgcgcca tattccgatg ggtgtaccgc
    50641 tacagaccga aatgcacgcg tatatggaga tcctgaaaaa atatccacca cgcgcgcaga
    50701 ttaaatctga ctaagccggc gctatcgctg agatctgcct ttgccggatg cgatgctgac
    50761 gcatcttatc cagcctacag aacgctgcaa tttattgaat ttgcacgatc atgtaggccg
    50821 gataaggcgt ttacgccgca tccggcaatc aaccgcaggc ggccgccgat ttctacttac
    50881 tcaccaccag caaatgcgca tgcataatgt cgctggccgg gcgaccgtgc gccagcaaat
    50941 cagccattgc tttaagatat ggcggtagat ggcggaaata acgctgatac ccggcacaca
    51001 aataattcag tcccggtttg ccgctggcat cgagcatgaa gcggtgtttc gggcagcctc
    51061 cccagcacgc ttttaacacg ttacaactgc gacactgcgc cggtaactgc ttaaatttat
    51121 cttcaccaaa cgcctgctgt tgcggggaat cgatcatttc tgcaattgtt tgctggtgca
    51181 tattccccag ccaatattgc ggataaacat agtgatcaca ggcgtaaacg tcgccgttgt
    51241 gctcaacaat caccgagcgc ccacaggttg gctgatgatg gcaaaccgca cccggcgcac
    51301 cgacaaaatt ggcaaacgcc cattcgatat tcatcacgaa aatcttgccg acgtcgcgtt
    51361 tgatccagtg gtcgaatatc gccaccagaa actcaccgaa ctcctcgggg cgcaccgacc
    51421 attccgttag ctcaccctga atatcaccag gcgcatgtaa cttaagtcca tcgcgggcag
    51481 ttgtttcatc agccaggcgc tcgaccaccg gaataaactg gatgaattcg actcccgcat
    51541 cgcacaaaaa atcatatacc tgcaacggtt gctgcgcgct ggtgcgatta acgcagacca
    51601 gcacgttata gtcgacatga tgtttttgca ggagcgtcag ggcacgcatc accagcttat
    51661 gcgtgggtct gccaccttta gtcacgcgat attgattgtg gatctcaggc gggccatcca
    51721 gcgataaacc aacaaggggg aaccgcagaa ttcggaaaaa atcgtacgct aagctaacgg
    51781 tgttctcgtg acagctcttt gactaggctt tctaaggcca ttctgatagc cctgacttcc
    51841 tgaaaagcca tggctaaaat ttgtgcggct aaaagggata accgatggta aagtaagtta
    51901 tccctgtcga gatactgaaa agcgttatcc tcgtttttcc caaaactgtt ttgccagttc
    51961 gctcagagcg ctagttaact gagcgacaga tttcgcactt tgcaaattat tctgcccggt
    52021 ctgtacattg gtatcagcag catcgcgtat attgataata ctgcggttta tgtcttcact
    52081 tactgctccc tgctgctcga ccgcagtcgc tatttgcgcg ttcatgtcgg taatttcgtt
    52141 aacgcgttgg ccaattccat caagagctgt agctgcttcc tctgcgtgag ctacactcgt
    52201 gtgcgcttgc cgactacttt gctccatgac tgtaacagcg gattgcgctc gctcttgtag
    52261 agcgctgatc atgctttgaa tatccgttgt cgattgctgt gtgcgagcag caagactgcg
    52321 aacctcatcg gcgacaacag caaaaccacg cccctgctca ccagcacgcg cggcctcaat
    52381 tgctgcgttg agtgccaaca aattcgtttg ctcggcgatc cctcgtataa cgtcaagaac
    52441 ttttgatatc tcgttacttt gaccttcaag ctcatgaata acctgagtgg cttgcctaat
    52501 ttcaccttca agggcagtga ttgactggct tgtgtgggct accagacgct ggccagatgc
    52561 cgtctcagtg tctgctcttc cggccgcatc tgcagcatgc tgtgcattgc tcgcaacctc
    52621 ttgaatgctt gccaccattt ggtttactgc cgttgctatt tgatctgtct ctgcctgctg
    52681 ctcaactgta agtacattgc ttgactcaat atcctttagt aggcctcggg tgtgttcgct
    52741 aagccgattt gatgcatcac ctatgcgacc tactatggcg cctgtttcag cttgcatcat
    52801 tcgtaaagca aactctattt ggccaaactc atcggtgcgc ccagtgtaga gggattgact
    52861 taatgggtta ttggaaatat tcctggctct ttcaaccagt cttccaagag gagagagaat
    52921 agccaaaaca ctaacagagc ttaagcttcc tgacattaaa gtggctaaca ataagctgct
    52981 tattgatgta tcagtaagca tgccggcagc cattgcgctt gatataatac taccccatat
    53041 gagcaagagt attttcacgg aaaagctagc agccaatttc ggcctcgcgg ccttcccgct
    53101 tctcaattga gcatataatt tttccgcagc caaaacctgc tcaggttcag gcttggtcct
    53161 tacagactgg tattcaacaa tcgaaccatt cttagctatt ggcgttacat aagcacttac
    53221 ccaatagtgg tcgccatttt tacagcgatt ttttactagc cccatccatg agcggccaga
    53281 ttttaatgta ctccacatat gctcaaatgc agcaggcggc atatctgggt gtcttacgat
    53341 gttgtgaggc tggcctaata gttcttcctc agtgaaacca ctgattttaa tgaagtcagg
    53401 attaacgtac gtgatatggc tttgagggga ggtagtcgaa agaatattgg catcttttgg
    53461 gagttctaag tttcgacccg tcactggtaa attctggcgc ataagaacct caagggttgg
    53521 ctgttttatt ttattgtttt cggcattaag cccaatttct tgagcgttac gataaagcta
    53581 gcatggaaac gataggtgca agcaagttaa gggttgcatc gcgcatgtca atctaggcta
    53641 taccctaact tgatgtcagg cagggccgcg ccgcttcgtc agaatagagt ctgctttccc
    53701 attttttgac acatgcccgc gaaggttata gatttcagcc tgacagaaat gggctttgag
    53761 gcacaacgga acagaaagtg cacttaagcc gccttcaacc aaggagacat cgtgcagggg
    53821 caccgcatcg gctacgtccg ggtcagcagc tttgaccaga acccggaacg ccagctggaa
    53881 caaacccagg tgagcaaggt gttcaccgac aaggcatcgg gcaaggacac ccagcgcccc
    53941 cagctcgaag cgctgctgag cttcgtccgc gaaggcgata cagtggtggt gcacagcatg
    54001 gatcggctgg cccgcaacct cgatgacctg cgtcgcttgg tacagaagct gactcagcgc
    54061 ggcgtgcgca tcgagttcct gaaggagggc ctggtgttca ctggcgagga ctcgccgatg
    54121 gccaacctga tgctgtcggt gatgggggcc ttcgctgagt tcgagcgcgc cctgatccgc
    54181 gagcggcagc gtgagggcat cgccttggcc aagcagcgtg gcgcgtaccg gggccgcaag
    54241 aaagccctgt ccgatgagca ggctgctacc ctgcggcagc gagcgacggc cggcgagccc
    54301 aaggcgcagc ttgcccgcga gttcaacatc agccgggaaa ccctctacca gtacctccgc
    54361 acggacgact gacacatgcc gcgtcgcttg atcctctcgg ccacggagcg ggacaccctg
    54421 cttgcgctgc cggaaagcca ggatgacctg atccgctact acaccttcaa cgactccgac
    54481 ctgtcgctga tccgccagcg acgcggcgac gccaaccgcc tcggcttcgc cgtgcagctc
    54541 tgcctgctgc gctaccccgg ttacgcgctg ggaaccgaca gcgagctgcc cgagccggtc
    54601 atcctgtggg tggcgaagca agtccaggcc gagccggcga gctgggcaaa gtacggcgag
    54661 cgcgacgtga cccgtcgcga gcatgcccag gaactgcgca cctacctgca actggccccg
    54721 ttcggcctgt ccgacttccg cgccctggtg cgcgagctaa ccgagctggc ccagcagacc
    54781 gacaaaggct tgctgctggc cggtcaggcc ctggagagcc tacggcagaa acgacgcatc
    54841 ctgccggcgc tgagcgtgat tgaccgggcc tgctcggaag ccattgcgcg agccaatcgg
    54901 cgggtctacc gcgccctggt cgaaccactc acggactcgc atcgggccaa gctggacgag
    54961 ctgttgaagc tcaaggccgg cagcagcatc acctggttga cctggctgcg ccaggcaccg
    55021 ctgaaaccca actctcggca catgctggaa cacatcgagc ggctgaagac atttcagttg
    55081 gtggacttgc ccgaaggcct gggccggcac atccaccaga accgcctgct caagctggcc
    55141 cgcgagggtg ggcagatgac gcccaaagac ctcggtaagt tcgagccgca gcgccgctac
    55201 gcgaccctgg ccgccgtggt gctggagagc accgcgaccg tgatcgatga gctggtcgat
    55261 ctgcatgacc gcatcctggt caagctgttc agcggcgcga agcacaagca tcagcagcag
    55321 ttccagaagc agggcaaggc gatcaacgac aaggtgcgcc tgtactccag gatcggccag
    55381 gcgctgctgg aagcgaagga aagcggcagc gacccctatg ccgccatcga ggcggtgatt
    55441 ccctgggacg agttcaccga gagcgtcagc gaggccgagc tgctggcccg gccggaaggc
    55501 ttcgaccacc tgcacctggt cggcgagaac ttcgccaccc tgcgccgtta cacgccggcc
    55561 ttgctggagg tgctggaact gcgcgccggg ccggccgcgc aaggcgtgct ggcagccgtg
    55621 cagaccctgc gtgagatgaa cgccgacaac ctgcgcaagg tgccggccga tgcacccacg
    55681 gccttcatca agccgcgctg gaagccgctg gtgatcaccc cggaaggcct cgaccggaaa
    55741 ttctacgaaa tctgcgccct gtccgagctg aagaacgccc tgcgctccgg cgacatctgg
    55801 gtcaagggct cgcggcagtt ccgcgacttc gacgactacc tgctgccggc cgagaagttc
    55861 gccgcactca agcgcgagca ggccctgccc ctggcgatca acccgaacag cgaccagtac
    55921 ctggaagagc gtttgcagct gctggacgag cagttggcca ccgtcacccg cctggccaag
    55981 gacaacgagc tgcccgatgc catcctcacc gagtcagggc tgaaaatcac cccgctggat
    56041 gcggcggtgc cggatcgggc gcaggcgctg atcgaccaaa ccagccagtt actgccgcgc
    56101 atcaagatca ccgaactgct gatggacgtg gacgactgga cgggcttcag ccgccacttc
    56161 acccacttga aggacggggc cgaggccaaa gacaggacgt tgctgctgtc cgcaatcctc
    56221 ggtgatgcga tcaacctcgg gctgaccaag atggccgagt cgagccccgg cctgacctac
    56281 gccaagctgt cctggctgca agcctggcac atccgcgacg aaacctattc ggcggccttg
    56341 gccgagctgg tcaaccacca gtatcgccac gcctttgccg cccactgggg cgacggcacg
    56401 acctcatcct ccgatggcca gcgcttccgc gcgggtggcc ggggcgagag caccgggcac
    56461 gtcaacccga agtacggtag cgagccggga cggctgttct atacccatat ctccgaccag
    56521 tacgcgccgt tcagcacccg cgtggtgaat gtcggcgtcc gcgattccac ctatgtgctc
    56581 gacggcctgc tgtaccacga gtccgacctg cggatcgagg agcactacac cgacacggcc
    56641 ggcttcaccg atcacgtctt tgccctgatg cacctgctag gcttccgctt cgcgccgcgc
    56701 atccgcgacc tcggcgaaac caagctgtac gtgccgcagg gcgtgcaagc ctacccgacg
    56761 ttgcgcccgc tgatcggcgg caccctgaac atcaagcacg tgcgtgccca ctgggacgac
    56821 atcctgcgcc tggccagctc gatcaagcag ggcaccgtca ccgcctcgct gatgctgcgc
    56881 aagctcggca gctacccgcg ccagaacgga ctggccgtgg ccctgcgcga gctgggccgg
    56941 atcgagcgca cgctgttcat cctggactgg ctgcaaagtg ttgaactgcg ccgccgcgtg
    57001 catgccggcc tgaacaaagg tgaggcgcgc aactcgctgg ccagggcggt gttcttcaac
    57061 cgccttgggg aaatcaggga tcggagcttc gagcagcagc gctaccgggc cagcggcctc
    57121 aacctggtga cggcggctat cgtgctgtgg aacacggtgt acctggaacg cgccacccag
    57181 gggttggtcg aggccggcaa gccggtggac ggcgagctgc tgcaattcct gtcgccgctg
    57241 ggctgggagc acatcaacct aaccggcgat tacgtctggc ggcagagccg cagactggaa
    57301 gacgggaagt ttcggccctt acggatgccc ggaaaacctt agcgtacgat tttttccgaa
    57361 ttctgcgggc tccccaacaa gaaaatgatg ctccgcgaga aacgcgcacc attcgtcatc
    57421 cagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac catatttcgc
    57481 ctgtagcgct acggcacggc ggtaaaacgc cagcccgagt agcgttggtt cgccgccctg
    57541 ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac ggacatacgc
    57601 ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg attgtttttc
    57661 gagataaaaa cagtagtcac agttcagatt acaatcggaa ccgctcggtt tcgccatcac
    57721 atgaaaagca cgcgttggaa cctgttgcag catggtcgct ccttgcctga agtcatcctt
    57781 caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc gtttattgcg
    57841 aagggagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt
    57901 ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa
    57961 gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt taaagtcgtg
    58021 gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg
    58081 cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg tggctaatgg
    58141 gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga gctatagcca
    58201 ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc gggactaaat
    58261 ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc gctctatcca
    58321 gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt cccacccctc
    58381 tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt tttgctgctt
    58441 attcctccag tttatgcgct ttcccgtggc gattaaggcc aaaaacttta taaatcgccg
    58501 tcaccgccag cataaagatg atgccaacaa acagcgacat acgcgtatct tcattaaagt
    58561 acatgccgat caaaacgcaa atcaggaatg ccattgttac gtaattggcc cacgggaaca
    58621 ggattgagcg gaacggatgg ctggcaatcg ccgctttatg tgcacgccga aaacgcagct
    58681 ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca ctgtagacgt
    58741 agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca attagcagaa
    58801 tagcaataga tactgccaca cccgcaaccg gtacgccgtg acgggaaact ttcgccattg
    58861 ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat acgtccgcaa ctgtacatgc
    58921 cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg cccgccgctg
    58981 cggtaatacc gattttggca aaagtcagta cgaacgggct gccgttgctg cctatttcat
    59041 tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac gtagaaaatc aggatccgcc
    59101 acagcacctt gcctacggca ctgcgcagcg tcacctgcgg attcttcgct tcaccggcag
    59161 taatgccaat cagctccacg ccctggtagg acgccaccac aatacacaga gcggtcagga
    59221 accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta aaaccaatcg
    59281 actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca atcatcacga
    59341 taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac aaccgcaccg
    59401 ccgccagatt cgccaacgcc accagcgcca ctgcgatcaa tgcgggtatc cactgcgcca
    59461 tctccgggaa ccagaactgg acataaacgc caatggcggt gatttcagag atccccaccg
    59521 ccatccacat aaaccagtaa gaccaggcgg tgagatagcc aaagaacggg ctcatataac
    59581 gatgcgcata aacggcgaac gaaccggtaa ccggttcgag gaacaacatt tcgcccattg
    59641 aacgcatgat gaaaaagacg aacagcccgg cgatgatata ggccaacaat acggatggcc
    59701 cggcccattt cagggtactg gcggccccca taaacaggcc gacgccaatg gtgcccccca
    59761 gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt ttgttatctg
    59821 ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc gttatgggta
    59881 catcgagtgt tgcaaatgtt ttcgtaattc aggagaaatg gcaaataaag cattaaaaat
    59941 ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc cgtcgcttta
    60001 caaagagagg aaaatcatag gttgccggtg tagtgccagc gtaaataacg cagcaaacga
    60061 agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc cagccccagc
    60121 gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta agtcccgcca
    60181 acgcccatat acagcgcatc tggatgtacc agacggcagt cgcgcatgat gatctcctgc
    60241 tttggcgatc ccatcgcaac ggtgacgatt tgcgcaccgc tggcatgaat gcgttcaaac
    60301 agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat attcacattc
    60361 cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc gcccacaaga
    60421 aataccggcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag atcggcaccg
    60481 gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac aacgctgatg
    60541 ccatccgcat atttaaattc ggcagcgtta attaactccc tgacctcggc gttatcttca
    60601 atagtcagca ttttttcagc attaatggca accagcgttc cctgcttaag ctgcccgtca
    60661 gcaaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg taagccacgc
    60721 agcgtatagg ttggtgccgt ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga
    60781 gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag tacaacagtt
    60841 ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gaccacgcgt gagacaaacg
    60901 aatccagccc ttcacgcgcc agcacgatca tattgaagat cgccccaaag cagaaactgt
    60961 gcaatatcgc agctttatag cgattaggct cgcggttgcc cagctcatac agccagtcga
    61021 accatttgat gatcagacca accacgatcg ccccgagcgg gatgaacaac gcgccgccca
    61081 tcaccaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg ttattcagca
    61141 cttcccaggt aaagtagttg gctgagttca gcaccatact cgggcgaccc ggccacagcc
    61201 aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg aagtcgatgt
    61261 tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgagaaggtg tcgcgagtga
    61321 gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag cgttttagtg
    61381 ccagccagaa catgccaaca atccccagca cgcccgccgc cgccagcatc cacaacgaaa
    61441 tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg atattggcgc
    61501 gagtgccgcc gacaatcata taagtcagca agccaaaggc gaccgtgctg acgaggaaaa
    61561 acagccacgc tttgctgtcc tggcgcagaa agtagaccac cagcatcgcc gggatgaaaa
    61621 agtaaaagaa gcgttttaac gccacgccgg agacttcact ggaaaagatc tgactgtagg
    61681 agttaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg cttaccagcg
    61741 cgatacccat caggatcacc cacgtaagat tggtctccac gcggttcatg gtaaacagcg
    61801 gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt tttgtaggtg acatagtaaa
    61861 ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa gatttctggc ggcgcgacac
    61921 caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa gccgaagaaa aaggtgagca
    61981 aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac tcaaaccagg
    62041 tcagcgtggc aataaacagc gtgcagagca gccagacaac aaacaggcca ctgaattgca
    62101 gcagactcat gcgacctccc cggcggcaat cgccaacgcc cgctgccagc cttgtagata
    62161 gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc gctgcgcttc
    62221 acgcacaata tcctcgttga gatcgtcggt agtaaacagc accggcaaat gttgttccgt
    62281 catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg cctgaatcag
    62341 taagcacagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccaa gatcgcactg
    62401 acgaagtagc gccagatagg cgtcaaattc cagtttttcg ctcagaattt gtagattttc
    62461 ttcgctgaat aactccagcc ccgcctgacg aacttcctca atgtacgctt cgttattagg
    62521 cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat gaacggcgcg
    62581 caaggcagca atatgctcat tgctgcggtc gccggagttc cccaccagaa tggtcatttt
    62641 cccttcacgt tgccgatcgt tcgccatcgt attgagcgaa gggtccatcc gcgtcgggaa
    62701 gaacagcagt tcgccccgca cctttgggtg cgttttggca aaaaagctca aatcgccgcg
    62761 ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagtgggt aaaaaagctt
    62821 atatctcaag ccactggaaa gctcatacag gtctgccccc cagatatgcc agaaaaactg
    62881 gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga actgaccgtg
    62941 gaagaaaaaa cgctgctgac ggttagcttt tgctttcgcg atgaccgctt ccgccagcga
    63001 ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta agccgtcgtc
    63061 cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcggccagcg cgtcattgaa
    63121 aaaccgcaaa acggttcggt tatggtgagg gatatccgat cccagtacgt gaatcagtac
    63181 agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa atagacgata
    63241 taagttgcca tatatgcctg cgccgcgccc agtgcaccat gcgcagggat tagccagtgg
    63301 gcaaatacca tcaataaagt gaactggctg acttccgcca gaatataaaa ccgcagtgac
    63361 gctttggcga tcaccagata accaaagaca taagcgccca cttttaacac atcacccact
    63421 aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag cagccagata
    63481 gcaaaatcac gcagcagcca gacggtaaaa ctcgccgccg ccactgccgg taagacgaat
    63541 ttcagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg cgacaacgtg
    63601 ggcagcaaat atacgctgaa cgatgccgta ataaattgca ggtaggcatc ggaaatactg
    63661 ctcacccctt gccagatccc cacctcatcc cagctatact gcgccgccag cagtttacgc
    63721 atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag cgtaaatttg
    63781 ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa cgggatgaca
    63841 ccacgtttga tcaacatgat ggcggcagga attaccacca gcgcgggaat cagcgccaga
    63901 cccagcaacg ccccttcata accgcccaaa cggtaactga cgtagtacgc gagaacgcca
    63961 atcaagctgc cgacaatcag cgataacgca ttacctgcgg catcgcgaaa gcctttcatc
    64021 agcgccagta acaggttgcc ccaggcgatc cccatttgca ccagcgccac taaacgcacc
    64081 agcccctgat agtcggtatt accaaacaat ccctggctga ttggcgcagc tgccagcaca
    64141 aaaaccagcg ccatcagcgt agagaaacca agtaccatcg ctgatgaagt gccgaccacg
    64201 cggcgcagct gttgcggatt atcatggtac tgggcaacgt atttggttac accgttaaag
    64261 atgccagccc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt tgccgccagc
    64321 ccaagccccg ccggaccaaa tgacaccgcc agcaacttac cgaccagtaa cccggcacca
    64381 atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga catatcagga
    64441 aaagtagttc aacaaagtcg caattaccgt acgctgattg acgggcgaca ggttgtagaa
    64501 cagcggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt caccgtggaa
    64561 ctcaccaaag tgttcccccg cagggcaacc gtgcagcgga atgtaatgaa acaccgccat
    64621 gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcatcaatat cccgcagttt
    64681 aatgtagaac atatgcgcgt tctgcacgca gccatcggga atcgacggca gctcgatacg
    64741 cccggctttc gccagaggcg ctaacgcatc gtagtagttt tgccacagcg ccagacgttg
    64801 ctggttgata cgatccgctg cttccagttg cgcccacagg tatgcagctt gcagatcgga
    64861 catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac cacggaagaa
    64921 ctggctgcgg ttagtgccct tttcacggat gatctcggct cgttcgatta acgctttatc
    64981 gttaatcagc gtcgcgccgc cttcaccgcc cgccgtgtag tttttggttt catggaagct
    65041 aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg acatcacgcc
    65101 ctgagcggca tcttctacca caaacaaatt atgctttttc gccaacgcca taatggtgtc
    65161 catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt tgtcggtgat
    65221 cgccgcttca atcagcgttt cgtcgatgtt catggtgtcc gggcgaacat ccacaaaaac
    65281 gatttttgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt agctcggcat
    65341 gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca gcgaagcggt
    65401 gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc actgctggca
    65461 gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg actgcatata
    65521 gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat cacctgtata
    65581 accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta agcgcggcgg
    65641 tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc gcgagcatac gcccagttta
    65701 gcgccgtttg catcagctca gcacctgcac cgcgtccagc cagcaggcca attcgcgcat
    65761 ctgtcgcatt gagttcccgt aaagagacat agccgcgaat atcgccggac gccgcacgta
    65821 aaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa
    65881 agcgactgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg
    65941 cgctggctaa ctgacgtaat gcgggaatat cggtctcttg tgccactaca gcaccgctat
    66001 cactggcatt gttcacgggt agcgccaaat caacttcacc ttctaccagg gagaatccca
    66061 gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc tgcacccgtg
    66121 accacggcgc taacgcgtct ggcgtcagga gcggtgcttc agacgtaatg cgcacgatgg
    66181 cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg gcgcggacgg
    66241 gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga actggcagca
    66301 cgcttcacac cctcgtcatc gagccagccg ttacgccagg caatctcttc caggcaggca
    66361 atcttaaagc cctggcgttt ttccaccgtc tgtacaaagg tgctggcttc aatcaggctg
    66421 tcgtgagtgc cggtatccag ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg
    66481 cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg ctccgacggc
    66541 ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc
    66601 cagtttgact tcggctgttt tggcttttct tccagcgaga tagcgcggaa attgtcgtca
    66661 aactccacca cgccaaagcg ttccgggtcc atcacctgat agccaaaaac cgtcgcccct
    66721 tcggtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgaccgaa gaagatgtta
    66781 tcgcccagca ccagacaaga aggttcacca ttaaggaagg tttcaccgat gataaaggcc
    66841 tgcgccagac cgtccgggct gggctgttcg gcatattcca gctggatacc gaactcacta
    66901 ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat gatgagaatt
    66961 tcgcggatac cggccagcat caacaccgac agcggatagt aaatcattgg cttatcgtaa
    67021 atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt gccggaaccg
    67081 cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt cagacctaaa
    67141 cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agctttcatt agccagatac
    67201 cactgaaccg ttttacgcat tccactttca aaggtttcct gcggcagcca gccaagttca
    67261 cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg gtcagcgaca
    67321 aaggtgatca ggtcacgata gtgcgccacg ccgtgcggct tgttcggagc cagttcttcc
    67381 agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg accaccaata
    67441 ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg ggcgtgatct
    67501 tccacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag cgatttaccc
    67561 gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta agggccgtag
    67621 ttattcgagc agttggtgat aagcgtcggc agaccatagg tccgcagcca ggcgcgcacc
    67681 agatggtcgc tgctggcttt tgacgcggaa taagggctgc tcggcgcata cggcgtggtt
    67741 tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcagt ggagatatga
    67801 tgaaaacgga acgctgattt tttatcttcc gtcagcgcat tccagtaagc ccgcgccgct
    67861 tcaagcaatg tataagtccc gacaatgttg gtttcaataa acgctgccgg gccgtcaata
    67921 gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg atgctcagtg
    67981 aatacgcgtg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc aaagcgctcg
    68041 ctttgcgcga ccggtgccag cgacatcagg tttccggcgt aggtcagctt atcgaccact
    68101 accaccgcgt cgctcgtttc gttgatgata taacgcacca gcgccgagcc aataaacccg
    68161 gcaccacctg ttatcagaat ttttctcatc agcgccagac tcctttggca tcgacgacat
    68221 actgctgatg gacattgtcg ccattgataa ctttgaactg actatgatcg accagcatca
    68281 ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa agcccggtca
    68341 gttttttcgg caactggtgg atgttaggct caacaaccag agtttcgccg ctatgccact
    68401 gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg caggtcatca atattcggtt
    68461 taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg gtagccgcca
    68521 ggcaatcagc caccgccgct ttcacctgat cgataaccca gaacggtttg tgatcgttca
    68581 cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg ataaaccacg
    68641 gatcaacagc aatgcagtga ccgcccacgc cagggccagg ctgaagaata ttaacgcgag
    68701 ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg gcacaaatca
    68761 gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg gtgagcttac
    68821 acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa attttgtaca
    68881 gttcgctggc gcgggccgaa caaaccggcg tcataccacc aatcacgcga tcgtttttaa
    68941 tcagctcgac cattacctgt cctggtaaca cgcgttccgg gcagtaagca atgttgacgt
    69001 ccgcctgctc gcccacctgc tgcgggaaag tgagatccgg acgcatctct gctaaccatt
    69061 ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc agcgcgcctt
    69121 ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata tctggctcat
    69181 gatcgccctt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca actggcgtcg
    69241 tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacactcgcc aaatcaggtt
    69301 cgacgatatg gatttcgcca cgattgatgg tatcaaccgc atgttggttg atatcgacac
    69361 caattacctg tttttgccgt gaggcaaacg ctgctgccgt tggcagcccg atataaccca
    69421 gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt ttaacgcttc
    69481 cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc tcatagcttg
    69541 atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct gcttatccgt
    69601 gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg tatcgcgcat
    69661 caccagcaca ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg agtcggtcaa
    69721 aatcagccag gcgtggttca tcagccagac aaacggtaaa tactcctggg gatcgatcag
    69781 aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt tcgggttgag
    69841 atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcgatgtctg ccagcgcgtg
    69901 gcagatttct tcaaagccac gaccgaaact ctcacgcctg tgaccggtca ccagaatcat
    69961 ctttttatcg gggtcgataa acgggtaatt tgccgccagt tctgaacgca gcttgtcgct
    70021 gctcatcacc tggtcacgca cccataacag tgcatcaatg actgtattac cggtaatgaa
    70081 gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt cggttggaga
    70141 gaagtgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg gccacggcga
    70201 atagagatcg cccgtgcgca gaccagcctc aacgtgacca acaggaatac gctgataaaa
    70261 cgccgccagg ctggttgcca gcgtcgtcgt cgtatcgccg tgaaccagca cgacgtctgg
    70321 tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta tctctgtcag
    70381 gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa agagtttcag
    70441 cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct caaaaaaagg
    70501 atcttttgcc aacgcatgca ccaacggcgc catcttgatg gcttccgggc gcgtaccaaa
    70561 tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct ttgcgctcac
    70621 cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc ccgatcagcc
    70681 ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg cgttttaccg
    70741 gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccagg gttggaccaa
    70801 cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag gccggaccga
    70861 cggcctgtaa attttccagt cgagcctgaa gcattggacg cccaagcagg aacatttctg
    70921 aatcaggtaa ttcctcggca ggtacatctg tcgcactgcg cgaaatatta tgctgctcag
    70981 caattttcag cgcctgctca atgctgttca tccggcggtc gtagatggct ttcgccacct
    71041 cttcctgacg cttcacctga gctttcatct ggatggtacg cgccgcccat gcgcctttca
    71101 gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac tgacgtaaca
    71161 ggttattagc gtcaggcgcg gtttcggcaa taagcttcac gctgtcattg accgcgcggg
    71221 taaagtctcc ggggataaac tggatgttgt taatcatttc atccagcaac gccgcatcgg
    71281 ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt ttgcagccag aactctctgc
    71341 gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc atgaccgatg
    71401 gttggtcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat tgctgctgcg
    71461 agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgccgtc gagctccact
    71521 cctgacgagc aaaaaacgta tacgccagcg cgattaacgc aaacgccagc cccatgccaa
    71581 taatccatag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc agttcatttt
    71641 cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca tttatttggt
    71701 taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct taataaagcg
    71761 agcaactttc caggcacgct taatgcaata tccatagagg aagaatgcta gcaaaaagag
    71821 caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc caatggaagc
    71881 gagcagtgct gcggcaaggg taatcagcac aaacgcctga cgggaagtaa acccggcacg
    71941 catgatcaaa tggtgaatat gctgacggtc aggagagaat gggctcatgc ctttacgcag
    72001 gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc acaaagcggt
    72061 aaccgggctg atgggatggg ttttgccctg ggtcgtttcg agcaggatcc agataacggt
    72121 aaaaccaatc agcgtactgc ccgcatcacc cataaagact ttgtagcggc gacccaggat
    72181 accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc accagattgc
    72241 gaggctggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg agacgcagga
    72301 caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa tggccgccca
    72361 gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc aggagccaaa
    72421 gatataaccc aggctactga gataaagctt gccgaacacc atcataacaa tgccaacagc
    72481 ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca gcgcgccaat
    72541 gaaaacaagc acaccggcac aagcgagata gagagatgca tgcggaatat agtaatcgac
    72601 aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga gaggtatcaa
    72661 tccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt ttgccacctt
    72721 acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat cagtactcac
    72781 tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt ataaccacga
    72841 agacctttat tcagaaggga aagtccgttc caaacccacc agtcctgtgc aaaaattaac
    72901 catttgatat gcaaattgca taacatgcca gcaaattcca ggaattttcc taacatcgcc
    72961 agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg tggcgttttt
    73021 ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt ggtcttggtc
    73081 attgccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg gtgaatgatt
    73141 ttgcgcagga tccacatttt ctgcagttct tcctgagtcg tgagcagctc ttctttacgg
    73201 gtaccagaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat cttacgagag
    73261 aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt agataacttc gtccatttta
    73321 gaaccggtat cgataagcgc cgtcgcgata atggtcaggc tgccgccctc ttccacgtta
    73381 cgcgccgcac caaagaagcg tttcggacga tgcagggcgt tggcatccac accaccggtc
    73441 aacactttac ctgacgccgg aacaacggtg ttgtaagcgc gcgccagacg agtgatggag
    73501 tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttggcctt ctcgatcacc
    73561 atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc aacaacttca
    73621 ccttttacca gacgctgcat ctcggttact tcttccggac gttcgtcgat cagcagaacc
    73681 atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg cagcagcatg
    73741 gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc gataggtgat
    73801 gccagatcca gtacgcgagc agttaaatct tcagtagaac cgttaccacg ttccatacgc
    73861 agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt gcgggcgttt
    73921 tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg ttcaccttct
    73981 ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa acggcggatt
    74041 tggctagggg aaacgtagat gtcatcagga ccggcgaggt aggagctgtc tgcggaacgg
    74101 aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat atcttcgcca
    74161 ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat acgagccagg
    74221 ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt attctttaat
    74281 tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga acttaatgtt
    74341 gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat gtcttttcgc
    74401 tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag tgacgagcca
    74461 gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta caagattcaa
    74521 acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc acgccgggcg
    74581 tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg ttagcgtcga
    74641 ggaactcttt caactgacct ttagacagtg cacccacttt ggttgccgcc acttcaccgt
    74701 ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca gtgccagggt
    74761 tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca gcgatttcat
    74821 ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa tcgacgagga
    74881 tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg tgaataattt
    74941 tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt tgttggtgta
    75001 acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa agcaatagta
    75061 agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag ttttccgact
    75121 tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa agggtttcat aactgtacgc
    75181 ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc gggcaggcgc
    75241 aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat cttctctctc
    75301 atcctgcgat tgccgatcgc aaggtgaatc agccgcgtgc cttaattatg gcaccgacgc
    75361 gtgaacttgc cgtgcagatc catgccgacg cagaaccgct ggcggaagct actggcctga
    75421 agctgggtct ggcttacggt ggtgatggct acgacaaaca gctgaaagtg ctggaaagcg
    75481 gcgttgacat tctgattggc accacggggc gtttaattga ctacgccaag cagaaccaca
    75541 ttaacctcgg tgccattcag gtggtggtac tggacgaagc cgatcgcatg tacgatctgg
    75601 gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac cagcgcctca
    75661 acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc gagcagatga
    75721 acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt attaaagaag
    75781 agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg atcgaagaag
    75841 agtggccaga cagagcgatt attttcgcca acaccaaaca ccgttgtgaa gagatctggg
    75901 gccacctggc agcagatggt catcgtgtcg gtttattgac aggcgatgtc gcgcagaaaa
    75961 aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg gttgccaccg
    76021 acgttgccgc gcgtggtttg catattccgg cagtgacgca cgtctttaac tacgatttac
    76081 ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca ggcgcaagcg
    76141 gtcactctat cagcctggcg tgtgaagagt atgcattgaa tttgcctgct attgagacct
    76201 atattggtca ctcaattccg gtaagcaaat acaatccgga cgcattgatg accgatctgc
    76261 caaaaccgct gcgcctcacg cgcccgcgca caggcaatgg tccgcgtcgt actggcgctc
    76321 cgcgtaatcg tcgtcgttca ggttaaataa aaaatgtcat actcaaaatc attcaaactg
    76381 tatcaagatg gcaagagaat gaatcccgat gagcttactg tagtaagtga ttcaggtgaa
    76441 agaatgcagc caacacagag acagattgaa ggatgaagag tatgggttcc acctcgtcgc
    76501 tgtatgcagc cattgatctc ggttcgaata gttttcatat gctggttgtg cgcgaggtgg
    76561 ctggaagcat ccagacgctg acgcgaataa aacgcaaagt gcgtctggct gctggcctga
    76621 atagcgaaaa tgccctctcc aatgaagcaa tggagcgcgg ttggcaatgt ttgcgcctgt
    76681 ttgctgaacg tctgcaagat attcctccct cgcaaattcg cgttgtcgct acggcgacgt
    76741 tacgccttgc cgtcaatgcg ggtgatttta ttgccaaagc gcaggaaatt ctcggttgtc
    76801 cggtacaggt gatcagcggt gaagaggaag cacgtctgat ttatcagggc gttgctcaca
    76861 ccactggtgg tgccgatcag cgcctggtgg tggatatagg cggagccagt actgaactgg
    76921 taaccggcac gggtgcacaa accacctcgt tgttcagcct gtcgatgggc tgcgtcacct
    76981 ggctggaacg ctattttgcc gatcgtaatc tggggcagga aaattttgat gctgcagaaa
    77041 aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt acggtatcac ggctggaaag
    77101 tgtgcgttgg tgcttccggc accgtgcagg cgttacagga aatcatgatg gcacagggga
    77161 tggatgaacg cattaccctg gaaaagttgc agcaattgaa acagcgagcc attcattgcg
    77221 gtcggctgga ggaactggag attgacgggc tgacgctgga acgtgcgtta gtgttcccga
    77281 gtggtctggc gatcctgatc gccattttta ccgaactgaa tattcagtgt atgaccctgg
    77341 cgggcggtgc gctgcgtgaa ggcctggtct acggcatgtt gcatctggcc gtcgagcagg
    77401 atattcgcag ccgtacgctg cgtaatattc aacgccgctt tatgatcgat attgatcagg
    77461 cacagcgcgt agccaaagtt gcggctaact tcttcgatca ggtggaaaac gaatggcatc
    77521 ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca gcttcatgaa atcggcctga
    77581 gcgttgactt caaacaagcg ccgcaacacg ctgcttatct ggtgcgtaat ctggatcttc
    77641 ccggttttac ccccgcacag aaaaaattgc tggcgacgct actgctcaac cagactaatc
    77701 cggtcgatct ctcatcgctg catcagcaaa atgccgtacc gccgcgcgtc gcagaacaac
    77761 tctgccgttt actacgcctg gccatcattt ttgccagccg tcgccgtgac gatctcgtgc
    77821 cagagatgac attacaggct aaccatgaac tgttgacctt gacgcttccg caaggttggc
    77881 taacccaaca tccgctgggt aaagagatta ttgctcagga aagccagtgg cagagctatg
    77941 tccactggcc gctggaagtg cattaatgag taagtgccgg atgcgatgct gacgcatctt
    78001 ttccggcctt gattatttcc ctcgttttgc cgccatcatc gctttcagat tcgccagatg
    78061 gctttgccct ttctgcatcc gttcttcggc gctgaccact ttgcgctcct gttcccaaat
    78121 cagatcatcc tgcggcagct ccagcaaaaa gcggctcggc tccgggcgca ccagttcgcc
    78181 gtactgacgg cgttctttac acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc
    78241 gacataggcc agccgccgct cctcatcgat attatcttca tcgatgctgc tctggtgcgg
    78301 caaaaaccct tcttccatac cgaccatgta gacataagga aactccagcc ctttcgacgc
    78361 gtggagagtc atcagttgca cctgatccag ctcttcttca ctctcaccac gctccatcat
    78421 gtcgcgcaaa gtaaagcgcg tcaccacctg ggtgagcgtc atcggctcat ccagttcact
    78481 gccttccagc atctccgtca tccagctaaa cagttggttg acgttcttca tgcgcatttc
    78541 ggcggctttc gggctgggcg atgtttcgta cagccaggat tcataatcca tgccatggat
    78601 cagatcacgc accgcggcaa tcggctcccg ctccgccaga cgctggattt ctgccaacca
    78661 gtgagtgaag cgggtcaatg cttcataacc acgtccgcta agcgtctgac tcaggcccat
    78721 atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc gcccactcac ccagcttttt
    78781 cagcgtagcc gggccaatct ctcgcttcgg cgtgttaacg atacgcagaa atgcgctgtc
    78841 atcgtccggg ttagtcagca cgcgcagata agccagcaag tccttgattt caggacgaga
    78901 gaaaaacgac gtaccaccag atattttgta cgggatgcgg ttttgcatca ggaatttttc
    78961 aaacacccgc gactgatggt taccgcgata aagaatggcg taatctttgt actgcgtttt
    79021 attgacgaag tgatgggcga tcagctcgcc agtaacgcgc tcagcctcat gttcttcgtt
    79081 attcgcgctt aatactttta gctccgcgcc ataacccagt tcggagaaca gacgcttttc
    79141 aaagacgtgc gggttattgg cgatcaggat gttcgccgct ttcagaatac gcccggaaga
    79201 gcgatagttc tgctcaagct taatcacctt cagcgccgga aaatcctgac tcagcagcac
    79261 caggttttgc ggacgtgcac cgcgccagga gtagatcgac tggtcatcgt cacccaccac
    79321 ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagc tcatactggc tggtgttggt
    79381 gtcctgatac tcatccacca gcagatagcg aattttgttc tgccagcgct cgcggacttc
    79441 ttcattgcgt tgcagcagca acgtcggcaa taaaatcaga tcatcgaagt cgagaacgtt
    79501 acaggctttc aggtgtgcat catacagccc ataacaatgg gcaaaaatac ggtcccgctc
    79561 gccaatcgca cttgctgccg cctgggacgg tgttttgaga tcattcttcc agttagagat
    79621 ggtcgaaatc agttgttgca ggagaacttt gtcatcttca atcagcccct cggtcaactc
    79681 tttaagcaaa gcaagctgat cggtatcgtc aaacaacgag aagttcgctt tcatcccaag
    79741 cgccgcatac tcgcgtttga tgatatccag ccccaacgta tggaaagtgg agatcatcag
    79801 cccacgcgcc tctttgcgcc ccagcgtctg ccctacacgc tctttcatct cgcgcgctgc
    79861 tttattagta aaggtcaccg ccgcaatgtg ccgcgcctga taaccgcaac cgcggatcag
    79921 atgggcgatt ttattggtga taacacgagt tttaccggaa cccgcgcccg ccagcaccag
    79981 gcaggggccg gtaacgaatt cgacagcttg ttgttggccg gggtttagac gcataggtgt
    80041 attgctcaat cttcgaacgg ggggaggatt gtagcagaat cgcatttgaa tgtgaccgcg
    80101 tgtaagccgc taaccggttg gctgcggatg tttaaatcag agagagaaga ccgaaatcgg
    80161 agttcatcgt gaatccatta gacttagaaa atatcgggtc gtaacgttgt gttattactt
    80221 tttcagagtt tactaaggag aggtttactc gtggcgatta atttcagccc taaggttggt
    80281 gaaatactgg aatgcaactt tggtaactat cctgttagcc aaaatggacc gttcagcacg
    80341 acgtattacg atggccgcat tcctcctgaa atgataaaaa acaggttagt tgtcgtgctc
    80401 aacggaaaaa ttaatggcaa cgcatgcatc gtcgttccat tatcaaccac tcgcgaccag
    80461 acaagttaaa acgtggaatg catgttgaaa tagccagtaa tgttatcaat gacctgcaat
    80521 tctttgatca gcagattcgt tgggccaaag ccgacctggt acaacaagtc agtcgaaaca
    80581 gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca catgagttgg
    80641 ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc ataaattgac
    80701 aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa gttttctccc
    80761 cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta ccagccactc
    80821 cctgactgac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc tgacaattat
    80881 tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt gtaaaagaag
    80941 agaaactggc tctggatctt ctcgagcaga ttaagaacgg ggccgatttc ggcaagctgg
    81001 cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt gaattccgcc
    81061 agggtcagat ggttccggcg ttcgataaag tggttttctc ttgtccggta ctggagccga
    81121 ccggcccgct gcacacccag ttcggatatc acatcattaa ggtgctgtac cgcaactaat
    81181 agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg tgggagaggg
    81241 tcggggtgag ggcatcagcg cgcacttaac ccgcaacagc aatacgtttc atatctgtca
    81301 tatagccgcg cagtttctta cctacctgct caatcgcatg gctgcgaatc gcttcgttca
    81361 catcacgcag ttgcccgtta tctaccgcgc cttccggaat agctttaccc aggtcgcccg
    81421 gttgcagctc tgccataaac ggtttcagca acggcacaca agcgtaagag aacagatagt
    81481 taccgtactc agcggtatca gagataacca cgttcatttc gtacagacgc ttacgggcga
    81541 tggtgttggc aatcagcggc agctcgtgca gtgattcata atatgcagac tcttcaatga
    81601 tgccggaatc gaccatggtt tcgaacgcca gttcaacgcc cgctttcacc atcgcaatca
    81661 tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac tgcggcgcgg
    81721 tttcaaacgc ggttttgccg gtctcttcac gccaggtcag cagtttctta tcatcgttgg
    81781 cccagtccgc catcataccg gaagagaatt cgccggagat gatgtcgtcc atatgtttct
    81841 ggaacagggg tgccatgatc tctttcagct gttcagaaag cgcataagca cgcagtttcg
    81901 ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgtttcagt gcttcggtga
    81961 tggtttccca accgaactga atcagttttt ctgcgtatgc tggatcggta ccttcttcca
    82021 ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg atggtttgct
    82081 cgcccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcaca cccgcacggt
    82141 gaccaccggt tgcagccgcc caggctttgg caatcgccat gccttcgcct ttcggatcgt
    82201 tttccgggtg aacggcaatc agcgtcggta cgccgaaccc acgtttgtac tcttcacgca
    82261 cttcggtgcc tgggcatttc ggcgcaacca tcactacggt gatatcttta cggatctgct
    82321 cgcccacttc gacgatgttg aaaccgtgcg agtagcccag cgccgcgccg tctttcatca
    82381 gtggctgtac ggtgcgcact acatcagagt gctgcttgtc cggcgtcagg ttaatcacca
    82441 gatccgcctg tgggatcagt tcttcgtaag tacccacttt aaaaccattt tcggtcgctt
    82501 tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag gagatatcga
    82561 gaccagaatc acgcatgttc aggccctggt tcagaccctg tgcgccacag ccgacgatga
    82621 ctactttttt accctgaagg tagctcgcgc catcggcgaa ttcatcgcgg cccataaagc
    82681 gacatttgcc cagctgtgcc agctgctggc gcagattcag tgtattgaag tagttagcca
    82741 tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg agattgaatt
    82801 cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca cattgcaatt
    82861 tttgcaacgt caacatcgag ggctgtccct gtggatttac gcgatctgaa aaccttcctg
    82921 catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt tagcccatcc
    82981 acgctctcac ggcagattca gcgcctggaa gaagatctcg gtcagccgct gtttgtgcgc
    83041 gataaccgca cggtgacgct gactgaagcg ggcgaagagc tgcgcgtttt cgcccagcaa
    83101 acgctgttgc agtatcagca gttgcgccac accatcgatc agcaagggcc gtcgctctct
    83161 ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc gccgattctg
    83221 gatcgcttcc gcgcggaaca cccgtcggtg gagattaaac ttactactgg tgatgcggca
    83281 gatgcgatgg aaaaggtggt cactggtgaa gcggatctgg cgattgcggg taaaccggaa
    83341 accttgcccg gcgcagtggc gttttcgatg ctggagaatc tggcagtagt gctgattgcc
    83401 cccgcgctgc cctgcccggt gcgtaatcag gtgtcggtag agaagccgga ctggtcaacg
    83461 gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact gtggtttcga
    83521 cgcaataaaa tcagtaaccc gatgatttac gccacggttg gcgggcatga agcgatggta
    83581 tcgatggtgg cactcggctg tggcgtggca ttgttgccgg aagtggtgct ggaaaacagc
    83641 cccgaaccgg tgcgtaaccg cgtgatgatt ttagagcgca gcgatgagaa aacgccgttt
    83701 gagctgggcg tgtgtgcgca gaaaaagcgg ctacatgagc cgctaattga ggcattctgg
    83761 aagattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa gcgaagcgct
    83821 atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    83881 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    83941 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    84001 aatgttccag tacgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    84061 gaattcgaag ctgtagaggc gttcctgcaa cggatgcgat ggacgtccgc cgaccatata
    84121 gcgcacgtgt agcttcgcca tttcgtcgtc ggagagatca accacgctgt agccgccgtc
    84181 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacaccgac
    84241 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgaacgccc
    84301 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    84361 caacgcttca cgctgttcgc ccagttcgca gcgttctgag acgtagcgca ggccgtggaa
    84421 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgcagggc
    84481 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcgcgcac
    84541 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    84601 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgctacgcc
    84661 ttcagcaaat agccctacgc gcggcagatc aaccggatga cccgcatcca gcgctgcttt
    84721 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    84781 gatcagcacc gccacgccag cagccagacc gccgccgccg actggcacaa atacgcggtc
    84841 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    84901 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    84961 ggctttcgct tcatcaaagt tcgcgccgtg gagcagcact tcgccgccga agccgcgcac
    85021 cgcgtcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    85081 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    85141 gtgcgctttc tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    85201 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    85261 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    85321 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    85381 tattaacccc ccagtttcga tttatcgcgc accgcgcctt tgtcggcgct ggttgccagg
    85441 ctggcataag cacgcagggc aaaggagacc tgacgttcac gatttttcgg cgtccaggct
    85501 ttgtcacctc gagcgtcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    85561 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc gatcaggcca
    85621 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgcca
    85681 ccagagaaac gaccgtcggt gatcagcgca caggctttgc cgagacccat tgatttcagg
    85741 aagctggttg ggtagagcat ttcctgcatc cccggaccgc ctttcgggcc ttcatagcga
    85801 attactacca catctccggc gacaacttta ccgccgagaa tcgcttctac cgcatcgtcc
    85861 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcctgcc
    85921 gttttcacga tgcagccgtt ttccgcaaag ttaccgtaga gcaccgccag gccgccgtct
    85981 ttgctgtagg cgtgttccag cgagcggata cagccattgg cgcgatcgtc gtccagcgta
    86041 tcccaacggc aatcttgcga gaatgcctgt gtggtacgaa tgcctgcagg acctgcgcgg
    86101 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagcgtttgc
    86161 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    86221 tcgccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttctgg
    86281 gtgctcggcg caactttaca cagctgtgga accttgcggg aaagcttatc gatatcactc
    86341 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac ggtgttagtc
    86401 gatccaccca tcgcgatatc cagcgtcatg gcgttttcaa acgccgcctt actggcgata
    86461 ttacgcggca gtgcactttc gtcgttttgc tcgtaataac gtttggtcaa ttcaacaatg
    86521 cgtttaccag cattaaggaa cagctgctta cggtcggcgt gggttgccag cagcgagccg
    86581 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    86641 atcccggagc aggaaccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    86701 tcagatactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag cttgatgatc
    86761 tgatcggaaa gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    86821 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    86881 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    86941 tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    87001 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    87061 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    87121 gcgataatcg gcttaccgaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    87181 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    87241 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    87301 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    87361 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa cctgaatacc
    87421 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaca cttcatccgc
    87481 caggtacagg gattcgcgcg acagcacctg ctcacgtact tcaattccca gctctttcgc
    87541 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    87601 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    87661 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    87721 cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt ttggtgctgc
    87781 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    87841 cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg gcgggtttac
    87901 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    87961 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg aaaccgggaa
    88021 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    88081 cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc catagtgcag
    88141 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    88201 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    88261 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    88321 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    88381 tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca cgatgacgca
    88441 ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca ttgacctgat
    88501 gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg gcaccagcgg
    88561 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    88621 catggtgtcg agtgccgctt caacctggtc tttccgggtg atgtgttggc catggatgcc
    88681 gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt cgctgtatcg
    88741 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    88801 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    88861 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    88921 aaccgccgcc ggtaaaccaa aacccatggt acctaaaccg ctggaggtga tgaaattttc
    88981 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    89041 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gcgcgtagat
    89101 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    89161 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    89221 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    89281 gataacactg gcgtgtggcg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    89341 tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggtgcc
    89401 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    89461 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    89521 ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    89581 ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    89641 ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    89701 accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    89761 cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    89821 ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga tggcaacaac
    89881 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    89941 agacgtggcg atacatacgc cagttttgcc ggtagcacga gcataaccga tagccgccat
    90001 tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat acaatgcatc
    90061 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    90121 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    90181 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    90241 aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct
    90301 ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg ctaatcacga
    90361 ccaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct tgttcgaagg
    90421 aatgcctaaa gagttaccat aggttttgta aatagtacaa gatatttttt ggccgatttt
    90481 tatgatgcgt ttaataattt caatataaat tgttgttttt taaaaataaa atagtgagtg
    90541 aaaaaaattc aatttcagta tgaatgttgt tcccttcctc gtagttcatc ctttgtccgc
    90601 tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg cacaaataaa
    90661 caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat cttatgcaca
    90721 taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc gcagccctgg
    90781 gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta cccggcttaa
    90841 cgatggtggg cttaccagaa acaacggtaa aagaagctcg cgatcgcgtg cgcagcgcca
    90901 ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctg gctccagctg
    90961 atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg ctggcggcct
    91021 cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa ctggcgctta
    91081 caggcgctct gcgtggcgtt cccggcgcaa tctccagtgc aactgaagct attaagtcgg
    91141 gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt aacggtgaag
    91201 gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt aagcacgctc
    91261 tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat ctcagtgatg
    91321 ttatcggtca ggaacaagga aagcgaggac tggaaattac cgctgctggc gggcacaacc
    91381 ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt attaatggcc
    91441 tgttgccaga tttaagcaat gaagaggcac tggagagtgc tgcgatatta agtctggtaa
    91501 atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct catcacagtg
    91561 catcgttaac tgcgatggta ggcggtggcg caattccagg gcccggtgaa atttcgctgg
    91621 cgcataacgg cgtgcttttt cttgatgagc tacctgaatt tgaacggcgt acactggatg
    91681 ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacacga gcaaaaataa
    91741 cctatccagc ccgtttccag cttgttgcgg cgatgaatcc cagccctacc ggacattatc
    91801 agggaaacca taaccgctgc acgccagaac agacattacg ttatctcaac cggctctcgg
    91861 ggccctttct cgaccgcttc gatctctcac tggagatccc attaccaccc cccggcattt
    91921 tgagtaaaac ggtagtgccg ggagaaagca gcgccaccgt taaacaacgc gtaatggccg
    91981 ccagagagcg ccaatttaag cggcagaata aactgaatgc ctggctggat agtccggaaa
    92041 tacgccaatt ctgcaaactt gagagcgaag atgcgatgtg gttggaagga acactgatcc
    92101 atctggggtt atcgattcgt gcctggcagc ggttattgaa agttgcacga accattgctg
    92161 atattgatca gtctgacatt atcacacgtc agcatttgca ggaggcagtt agctatcgag
    92221 cgattgaccg tttgctcatc catctgcaga aactactgac ataaaaaaag ggcatttcgc
    92281 cctttttatt aatcgtcaga atcggtgtag tcttcagcac cttcaacctg cggtttaccg
    92341 ccggaaaggg tgtgaaaacg ttttggacgc ttaatacgcg tcatatactt ggaccacacg
    92401 cgttctgctt ctgtcactgg ctcacgttcg ccacggcata ctgctacgaa gagtttctct
    92461 tcctcggtaa ccggctcgcg tttgccaaga tccaactcat tgaaggcata accatgacgc
    92521 tcaagcagtt gtgcctcttt gatggtgaaa tcaccatgac gagagaatcc acgtggataa
    92581 tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct gcacgctcct
    92641 aattctttga ccgagctagt tatggcgcgg agtattagtt acgcttgaca gagtgtaaaa
    92701 caaaacattt aaatcataac gacaaataat tttgcggaga gcactgtgga tacggaattg
    92761 ttaaaaactt tcctggaagt tagccgaacg cgtcactttg gtcgagcggc tgaatcgctc
    92821 tatctgaccc agtcagcagt gagctttcga atcagacaac tggaaaatca actgggtgtg
    92881 aaccttttca cccgccacag aaacaatatc cgtttaaccg ctgccggtga aaaactactg
    92941 ccttatgcag aaacgctcat gagcacctgg caggccgccc gtaaggaggt ggcgcatacc
    93001 tcacgacata acgagttttc tatcggtgcc agcgcctcgt tgtgggaatg tatgcttaat
    93061 cagtggctgg gacgcttgta tcaaaatcag gatgcccata caggcttaca gttcgaagcg
    93121 cgaattgccc aacggcagtc tctggtaaaa cagctgcatg aacgccagct tgatcttctt
    93181 attaccactg aagcgcccaa aatggacgaa tttagtagtc agttgctggg atatttcact
    93241 ttagcgcttt ataccagtgc cccttcaaaa ctaaagggag atcttaatta tctgcgactt
    93301 gagtgggggc cagattttca acagcatgag gcaggtttga tcggtgctga cgaagtgccc
    93361 attctgacaa ccagttctgc tgaactggca cagcaacaga ttgcgatgct taatggttgc
    93421 acctggctac ccgtcagctg ggcgcgtaaa aaaggcggcc tgcataccgt tgtcgatagc
    93481 acaacacttt cacggccgct ttatgccata tggctgcaaa atagcgataa aaatgcgttg
    93541 attcgcgatc ttttgaaaat taacgtgctg gatgaagtgt attaatatga atggctggca
    93601 aggatgccgg tagaaggatt tacttcggag agggttattt cagataaaaa aaatccttag
    93661 ctttcgctaa ggatgatttc tggcaggggc ggagagactc gaactcccaa cacccggttt
    93721 tggagaccgg tgctctacca attgaactac gcccctaatt ag
//
LOCUS       DBBGLQ010000008        89337 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.008, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000008 DBBGLQ010000000
VERSION     DBBGLQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.008"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            324..1301
                     /gene="glaH"
                     /locus_tag="ACIYSB_000762"
     CDS             324..1301
                     /gene="glaH"
                     /locus_tag="ACIYSB_000762"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGC5427866.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLTPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVAKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDNYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            1321..2589
                     /gene="lhgO"
                     /locus_tag="ACIYSB_000763"
     CDS             1321..2589
                     /gene="lhgO"
                     /locus_tag="ACIYSB_000763"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGC5427867.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYRDVTAAMAKIFQS
                     RGGEIIYNAEVSGLNEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2612..4060
                     /gene="gabD"
                     /locus_tag="ACIYSB_000764"
     CDS             2612..4060
                     /gene="gabD"
                     /locus_tag="ACIYSB_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGC5427868.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEAIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDNGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4074..5354
                     /gene="gabT"
                     /locus_tag="ACIYSB_000765"
     CDS             4074..5354
                     /gene="gabT"
                     /locus_tag="ACIYSB_000765"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGC5427869.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAVVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5592..6992
                     /gene="gabP"
                     /locus_tag="ACIYSB_000766"
     CDS             5592..6992
                     /gene="gabP"
                     /locus_tag="ACIYSB_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGC5427870.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7013..7675
                     /gene="csiR"
                     /locus_tag="ACIYSB_000767"
     CDS             7013..7675
                     /gene="csiR"
                     /locus_tag="ACIYSB_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGC5427871.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7676..8125)
                     /gene="kbp"
                     /locus_tag="ACIYSB_000768"
     CDS             complement(7676..8125)
                     /gene="kbp"
                     /locus_tag="ACIYSB_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGC5427872.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8209..8367)
                     /gene="yqaE"
                     /locus_tag="ACIYSB_000769"
     CDS             complement(8209..8367)
                     /gene="yqaE"
                     /locus_tag="ACIYSB_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGC5427873.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8550..8849
                     /gene="ygaV"
                     /locus_tag="ACIYSB_000770"
     CDS             8550..8849
                     /gene="ygaV"
                     /locus_tag="ACIYSB_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGC5427874.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8859..9383
                     /gene="ygaP"
                     /locus_tag="ACIYSB_000771"
     CDS             8859..9383
                     /gene="ygaP"
                     /locus_tag="ACIYSB_000771"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGC5427875.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9430..9834)
                     /gene="stpA"
                     /locus_tag="ACIYSB_000772"
     CDS             complement(9430..9834)
                     /gene="stpA"
                     /locus_tag="ACIYSB_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGC5427876.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10502..10951
                     /gene="alaE"
                     /locus_tag="ACIYSB_000773"
     CDS             10502..10951
                     /gene="alaE"
                     /locus_tag="ACIYSB_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGC5427877.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(10988..11332)
                     /gene="ygaC"
                     /locus_tag="ACIYSB_000774"
     CDS             complement(10988..11332)
                     /gene="ygaC"
                     /locus_tag="ACIYSB_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGC5427878.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            11484..11813
                     /gene="ygaM"
                     /locus_tag="ACIYSB_000775"
     CDS             11484..11813
                     /gene="ygaM"
                     /locus_tag="ACIYSB_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGC5427879.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            12061..12306
                     /gene="nrdH"
                     /locus_tag="ACIYSB_000776"
     CDS             12061..12306
                     /gene="nrdH"
                     /locus_tag="ACIYSB_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGC5427880.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            12303..12713
                     /gene="nrdI"
                     /locus_tag="ACIYSB_000777"
     CDS             12303..12713
                     /gene="nrdI"
                     /locus_tag="ACIYSB_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGC5427881.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            12686..14830
                     /gene="nrdE"
                     /locus_tag="ACIYSB_000778"
     CDS             12686..14830
                     /gene="nrdE"
                     /locus_tag="ACIYSB_000778"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGC5427882.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYTRTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            14840..15799
                     /gene="nrdF"
                     /locus_tag="ACIYSB_000779"
     CDS             14840..15799
                     /gene="nrdF"
                     /locus_tag="ACIYSB_000779"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGC5427883.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            16153..17355
                     /gene="proV"
                     /locus_tag="ACIYSB_000780"
     CDS             16153..17355
                     /gene="proV"
                     /locus_tag="ACIYSB_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGC5427884.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            17348..18412
                     /gene="proW"
                     /locus_tag="ACIYSB_000781"
     CDS             17348..18412
                     /gene="proW"
                     /locus_tag="ACIYSB_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGC5427885.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            18470..19462
                     /gene="proX"
                     /locus_tag="ACIYSB_000782"
     CDS             18470..19462
                     /gene="proX"
                     /locus_tag="ACIYSB_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGC5427886.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            19654..20838
                     /locus_tag="ACIYSB_000783"
     CDS             19654..20838
                     /locus_tag="ACIYSB_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5427887.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            20962..21699
                     /gene="ygaZ"
                     /locus_tag="ACIYSB_000784"
     CDS             20962..21699
                     /gene="ygaZ"
                     /locus_tag="ACIYSB_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGC5427888.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWIAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            21689..22024
                     /gene="ygaH"
                     /locus_tag="ACIYSB_000785"
     CDS             21689..22024
                     /gene="ygaH"
                     /locus_tag="ACIYSB_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGC5427889.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            22115..22645
                     /gene="emrR"
                     /locus_tag="ACIYSB_000786"
     CDS             22115..22645
                     /gene="emrR"
                     /locus_tag="ACIYSB_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGC5427890.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            22772..23944
                     /gene="emrA"
                     /locus_tag="ACIYSB_000787"
     CDS             22772..23944
                     /gene="emrA"
                     /locus_tag="ACIYSB_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGC5427891.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     ISPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            23961..25499
                     /gene="emrB"
                     /locus_tag="ACIYSB_000788"
     CDS             23961..25499
                     /gene="emrB"
                     /locus_tag="ACIYSB_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGC5427892.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(25563..26078)
                     /gene="luxS"
                     /locus_tag="ACIYSB_000789"
     CDS             complement(25563..26078)
                     /gene="luxS"
                     /locus_tag="ACIYSB_000789"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGC5427893.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(26228..27784)
                     /gene="gshA"
                     /locus_tag="ACIYSB_000790"
     CDS             complement(26228..27784)
                     /gene="gshA"
                     /locus_tag="ACIYSB_000790"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGC5427894.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(27857..28285)
                     /gene="yqaA"
                     /locus_tag="ACIYSB_000791"
     CDS             complement(27857..28285)
                     /gene="yqaA"
                     /locus_tag="ACIYSB_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGC5427895.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(28282..28848)
                     /gene="yqaB"
                     /locus_tag="ACIYSB_000792"
     CDS             complement(28282..28848)
                     /gene="yqaB"
                     /locus_tag="ACIYSB_000792"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGC5427896.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(29129..29205)
                     /locus_tag="ACIYSB_000793"
     tRNA            complement(29129..29205)
                     /locus_tag="ACIYSB_000793"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29169..29171),aa:Arg,seq:acg)
     gene            complement(29404..29480)
                     /locus_tag="ACIYSB_000794"
     tRNA            complement(29404..29480)
                     /locus_tag="ACIYSB_000794"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29444..29446),aa:Arg,seq:acg)
     gene            complement(29543..29619)
                     /locus_tag="ACIYSB_000795"
     tRNA            complement(29543..29619)
                     /locus_tag="ACIYSB_000795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29583..29585),aa:Arg,seq:acg)
     gene            complement(29818..29894)
                     /locus_tag="ACIYSB_000796"
     tRNA            complement(29818..29894)
                     /locus_tag="ACIYSB_000796"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29858..29860),aa:Arg,seq:acg)
     gene            complement(29898..29990)
                     /locus_tag="ACIYSB_000797"
     tRNA            complement(29898..29990)
                     /locus_tag="ACIYSB_000797"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29954..29956),aa:Ser,seq:gct)
     gene            complement(30306..30491)
                     /gene="csrA"
                     /locus_tag="ACIYSB_000798"
     CDS             complement(30306..30491)
                     /gene="csrA"
                     /locus_tag="ACIYSB_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGC5427897.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(30726..33356)
                     /gene="alaS"
                     /locus_tag="ACIYSB_000799"
     CDS             complement(30726..33356)
                     /gene="alaS"
                     /locus_tag="ACIYSB_000799"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGC5427898.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNTQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(33484..33984)
                     /gene="recX"
                     /locus_tag="ACIYSB_000800"
     CDS             complement(33484..33984)
                     /gene="recX"
                     /locus_tag="ACIYSB_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGC5427899.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQEI
                     WRNFAD"
     gene            complement(34053..35114)
                     /gene="recA"
                     /locus_tag="ACIYSB_000801"
     CDS             complement(34053..35114)
                     /gene="recA"
                     /locus_tag="ACIYSB_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGC5427900.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(35194..35691)
                     /gene="pncC"
                     /locus_tag="ACIYSB_000802"
     CDS             complement(35194..35691)
                     /gene="pncC"
                     /locus_tag="ACIYSB_000802"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGC5427901.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(35836..36921)
                     /gene="mltB"
                     /locus_tag="ACIYSB_000803"
     CDS             complement(35836..36921)
                     /gene="mltB"
                     /locus_tag="ACIYSB_000803"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGC5427902.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37177..37740
                     /gene="srlA"
                     /locus_tag="ACIYSB_000804"
                     /gene_synonym="gutA"
     CDS             37177..37740
                     /gene="srlA"
                     /locus_tag="ACIYSB_000804"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGC5427903.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            37737..38696
                     /gene="srlE"
                     /locus_tag="ACIYSB_000805"
                     /gene_synonym="gutE"
     CDS             37737..38696
                     /gene="srlE"
                     /locus_tag="ACIYSB_000805"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGC5427904.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSTVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            38707..39078
                     /gene="srlB"
                     /locus_tag="ACIYSB_000806"
                     /gene_synonym="gutB"
     CDS             38707..39078
                     /gene="srlB"
                     /locus_tag="ACIYSB_000806"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGC5427905.1"
                     /translation="MTVIYQTTITRIGASAIDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            39082..39861
                     /gene="srlD"
                     /locus_tag="ACIYSB_000807"
     CDS             39082..39861
                     /gene="srlD"
                     /locus_tag="ACIYSB_000807"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGC5427906.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            39966..40325
                     /gene="gutM"
                     /locus_tag="ACIYSB_000808"
     CDS             39966..40325
                     /gene="gutM"
                     /locus_tag="ACIYSB_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGC5427907.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            40392..41165
                     /gene="srlR"
                     /locus_tag="ACIYSB_000809"
                     /gene_synonym="gutR"
     CDS             40392..41165
                     /gene="srlR"
                     /locus_tag="ACIYSB_000809"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGC5427908.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            41158..42123
                     /gene="gutQ"
                     /locus_tag="ACIYSB_000810"
     CDS             41158..42123
                     /gene="gutQ"
                     /locus_tag="ACIYSB_000810"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGC5427909.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(42120..43634)
                     /gene="norR"
                     /locus_tag="ACIYSB_000811"
     CDS             complement(42120..43634)
                     /gene="norR"
                     /locus_tag="ACIYSB_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGC5427910.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRCLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            43821..45260
                     /gene="norV"
                     /locus_tag="ACIYSB_000812"
     CDS             43821..45260
                     /gene="norV"
                     /locus_tag="ACIYSB_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGC5427911.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCRWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            45257..46390
                     /gene="norW"
                     /locus_tag="ACIYSB_000813"
     CDS             45257..46390
                     /gene="norW"
                     /locus_tag="ACIYSB_000813"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGC5427912.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(46518..48770)
                     /gene="hypF"
                     /locus_tag="ACIYSB_000814"
     CDS             complement(46518..48770)
                     /gene="hypF"
                     /locus_tag="ACIYSB_000814"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGC5427913.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLYQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(48923..49450)
                     /gene="hydN"
                     /locus_tag="ACIYSB_000815"
     CDS             complement(48923..49450)
                     /gene="hydN"
                     /locus_tag="ACIYSB_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGC5427914.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(49599..50609)
                     /gene="ascG"
                     /locus_tag="ACIYSB_000816"
     CDS             complement(49599..50609)
                     /gene="ascG"
                     /locus_tag="ACIYSB_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGC5427915.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAP
                     SR"
     gene            50869..52326
                     /gene="ascF"
                     /locus_tag="ACIYSB_000817"
     CDS             50869..52326
                     /gene="ascF"
                     /locus_tag="ACIYSB_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGC5427916.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKTPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKYQSVQPTVAKEVSLN"
     gene            52335..53759
                     /gene="ascB"
                     /locus_tag="ACIYSB_000818"
     CDS             52335..53759
                     /gene="ascB"
                     /locus_tag="ACIYSB_000818"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGC5427917.1"
                     /translation="MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(53918..54388)
                     /gene="hycI"
                     /locus_tag="ACIYSB_000819"
     CDS             complement(53918..54388)
                     /gene="hycI"
                     /locus_tag="ACIYSB_000819"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGC5427918.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(54381..54791)
                     /gene="hycH"
                     /locus_tag="ACIYSB_000820"
     CDS             complement(54381..54791)
                     /gene="hycH"
                     /locus_tag="ACIYSB_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGC5427919.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(54788..55555)
                     /gene="hycG"
                     /locus_tag="ACIYSB_000821"
     CDS             complement(54788..55555)
                     /gene="hycG"
                     /locus_tag="ACIYSB_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGC5427920.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(55555..56097)
                     /gene="hycF"
                     /locus_tag="ACIYSB_000822"
     CDS             complement(55555..56097)
                     /gene="hycF"
                     /locus_tag="ACIYSB_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGC5427921.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGHCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(56107..57816)
                     /gene="hycE"
                     /locus_tag="ACIYSB_000823"
     CDS             complement(56107..57816)
                     /gene="hycE"
                     /locus_tag="ACIYSB_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGC5427922.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(57834..58757)
                     /gene="hycD"
                     /locus_tag="ACIYSB_000824"
     CDS             complement(57834..58757)
                     /gene="hycD"
                     /locus_tag="ACIYSB_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGC5427923.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(58760..60586)
                     /gene="hycC"
                     /locus_tag="ACIYSB_000825"
     CDS             complement(58760..60586)
                     /gene="hycC"
                     /locus_tag="ACIYSB_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGC5427924.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALVEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(60583..61194)
                     /gene="hycB"
                     /locus_tag="ACIYSB_000826"
     CDS             complement(60583..61194)
                     /gene="hycB"
                     /locus_tag="ACIYSB_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGC5427925.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(61319..61780)
                     /gene="hycA"
                     /locus_tag="ACIYSB_000827"
     CDS             complement(61319..61780)
                     /gene="hycA"
                     /locus_tag="ACIYSB_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGC5427926.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            61992..62342
                     /gene="hypA"
                     /locus_tag="ACIYSB_000828"
     CDS             61992..62342
                     /gene="hypA"
                     /locus_tag="ACIYSB_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGC5427927.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            62346..63218
                     /gene="hypB"
                     /locus_tag="ACIYSB_000829"
     CDS             62346..63218
                     /gene="hypB"
                     /locus_tag="ACIYSB_000829"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGC5427928.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            63209..63481
                     /gene="hypC"
                     /locus_tag="ACIYSB_000830"
     CDS             63209..63481
                     /gene="hypC"
                     /locus_tag="ACIYSB_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGC5427929.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            63481..64602
                     /gene="hypD"
                     /locus_tag="ACIYSB_000831"
     CDS             63481..64602
                     /gene="hypD"
                     /locus_tag="ACIYSB_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGC5427930.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVQQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            64599..65609
                     /gene="hypE"
                     /locus_tag="ACIYSB_000832"
     CDS             64599..65609
                     /gene="hypE"
                     /locus_tag="ACIYSB_000832"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGC5427931.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            65683..67761
                     /gene="flhA"
                     /locus_tag="ACIYSB_000833"
     CDS             65683..67761
                     /gene="flhA"
                     /locus_tag="ACIYSB_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGC5427932.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLDAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVALEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(67798..68151)
                     /gene="ygbA"
                     /locus_tag="ACIYSB_000834"
     CDS             complement(67798..68151)
                     /gene="ygbA"
                     /locus_tag="ACIYSB_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGC5427933.1"
                     /translation="MSGKRISREKLTIKKMIDLYQTKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPHE"
     gene            complement(68228..68362)
                     /locus_tag="ACIYSB_000835"
     CDS             complement(68228..68362)
                     /locus_tag="ACIYSB_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427934.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            68438..70999
                     /gene="mutS"
                     /locus_tag="ACIYSB_000836"
     CDS             68438..70999
                     /gene="mutS"
                     /locus_tag="ACIYSB_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGC5427935.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            71105..71761
                     /gene="pphB"
                     /locus_tag="ACIYSB_000837"
     CDS             71105..71761
                     /gene="pphB"
                     /locus_tag="ACIYSB_000837"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGC5427936.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPEID
                     LLISVGDNIDRGPESLDVLRLLNQPWFTSVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYAIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPNSGRLSFYKIK
                     "
     gene            complement(71812..72579)
                     /gene="ygbI"
                     /locus_tag="ACIYSB_000838"
     CDS             complement(71812..72579)
                     /gene="ygbI"
                     /locus_tag="ACIYSB_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HGC5427937.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALVKQDLSLLVAKNE"
     gene            72775..73683
                     /gene="ltnD"
                     /locus_tag="ACIYSB_000839"
     CDS             72775..73683
                     /gene="ltnD"
                     /locus_tag="ACIYSB_000839"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HGC5427938.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLLGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTIMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            73680..74942
                     /gene="otnK"
                     /locus_tag="ACIYSB_000840"
     CDS             73680..74942
                     /gene="otnK"
                     /locus_tag="ACIYSB_000840"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HGC5427939.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQARKAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDDAFFSRAQREFLS"
     gene            74939..75577
                     /gene="ygbL"
                     /locus_tag="ACIYSB_000841"
     CDS             74939..75577
                     /gene="ygbL"
                     /locus_tag="ACIYSB_000841"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGC5427940.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            75582..76358
                     /gene="ygbM"
                     /locus_tag="ACIYSB_000842"
     CDS             75582..76358
                     /gene="ygbM"
                     /locus_tag="ACIYSB_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGC5427941.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            76447..77811
                     /gene="ygbN"
                     /locus_tag="ACIYSB_000843"
     CDS             76447..77811
                     /gene="ygbN"
                     /locus_tag="ACIYSB_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HGC5427942.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(77905..78897)
                     /gene="rpoS"
                     /locus_tag="ACIYSB_000844"
     CDS             complement(77905..78897)
                     /gene="rpoS"
                     /locus_tag="ACIYSB_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGC5427943.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(78960..80054)
                     /gene="nlpD"
                     /locus_tag="ACIYSB_000845"
     CDS             complement(78960..80054)
                     /gene="nlpD"
                     /locus_tag="ACIYSB_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGC5427944.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(80239..80865)
                     /gene="pcm"
                     /locus_tag="ACIYSB_000846"
     CDS             complement(80239..80865)
                     /gene="pcm"
                     /locus_tag="ACIYSB_000846"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGC5427945.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(80859..81620)
                     /gene="surE"
                     /locus_tag="ACIYSB_000847"
     CDS             complement(80859..81620)
                     /gene="surE"
                     /locus_tag="ACIYSB_000847"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGC5427946.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(81601..82650)
                     /gene="truD"
                     /locus_tag="ACIYSB_000848"
     CDS             complement(81601..82650)
                     /gene="truD"
                     /locus_tag="ACIYSB_000848"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGC5427947.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(82647..83126)
                     /gene="ispF"
                     /locus_tag="ACIYSB_000849"
     CDS             complement(82647..83126)
                     /gene="ispF"
                     /locus_tag="ACIYSB_000849"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGC5427948.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(83126..83836)
                     /gene="ispD"
                     /locus_tag="ACIYSB_000850"
     CDS             complement(83126..83836)
                     /gene="ispD"
                     /locus_tag="ACIYSB_000850"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGC5427949.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(83855..84166)
                     /gene="ftsB"
                     /locus_tag="ACIYSB_000851"
     CDS             complement(83855..84166)
                     /gene="ftsB"
                     /locus_tag="ACIYSB_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGC5427950.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(84360..84683)
                     /gene="ygbE"
                     /locus_tag="ACIYSB_000852"
     CDS             complement(84360..84683)
                     /gene="ygbE"
                     /locus_tag="ACIYSB_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGC5427951.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(84733..85338)
                     /gene="cysC"
                     /locus_tag="ACIYSB_000853"
     CDS             complement(84733..85338)
                     /gene="cysC"
                     /locus_tag="ACIYSB_000853"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGC5427952.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRYFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(85338..86765)
                     /gene="cysN"
                     /locus_tag="ACIYSB_000854"
     CDS             complement(85338..86765)
                     /gene="cysN"
                     /locus_tag="ACIYSB_000854"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGC5427953.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDTRKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEKTFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMAWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(86767..87675)
                     /gene="cysD"
                     /locus_tag="ACIYSB_000855"
     CDS             complement(86767..87675)
                     /gene="cysD"
                     /locus_tag="ACIYSB_000855"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGC5427954.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            87927..88964
                     /gene="iap"
                     /locus_tag="ACIYSB_000856"
     CDS             87927..88964
                     /gene="iap"
                     /locus_tag="ACIYSB_000856"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGC5427955.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   89047..89319
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=89108..89136
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT        21008 a        23951 c        23196 g        21182 t
ORIGIN      
        1 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgcagtg
       61 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
      121 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
      181 tactaatttg ttgcttttga tcacaataag aaaacaatat gtcgcttttg tgcgcatttt
      241 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
      301 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
      361 attcaggcca ggactatagc ggattcaccc tcaccccgtc ggcgcaatcc ccgcgtctgc
      421 tggaactcac cttcaccgaa cagacgacca aacagtttct tgagcaggtt gccgagtggc
      481 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtagccaaa attcttgacg
      541 atctatgtgc gaaccagctg caaccattgc tgttgaagac gttgctaaac cgcgctgaag
      601 gtgcgttgtt gatcaatgcg gtgggtgtcg atgatgtgaa gcaagcggat gagatggtga
      661 agctggcgac ggcggtggcg catctgattg gccgttccaa tttcgatgcg atgagcggtc
      721 agtattacgc gcgttttgta gtgaaaaatg tcgataactc agacagctat ctgcgccagc
      781 cgcaccgcgt gatggagcta cacaacgacg gcacgtatgt cgaagagata accgattacg
      841 tgctgatgat gaaaatcgac gagcaaaaca tgcagggcgg aaattcgctg ctgctgcatc
      901 tcgatgactg ggaacatctc gacaactatt tccgccaccc gctggcgcgt cgcccgatgc
      961 gctttgccgc gccgccgagt aaaaacgtca gcaaagatgt ttttcatccg gtgttcgacg
     1021 tcgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccgaaagact
     1081 tcgaagaagg cgtgtggttg agcgagcttt ccgacgccat tgaaaccagc aaaggcattc
     1141 tttctgtgcc cgttcccgtt ggcaaattcc tgttgattaa caacctgttc tggctgcacg
     1201 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgc cagcgtggct
     1261 atttcgctta cgcctctaac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     1321 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     1381 attgatgtct atccggacgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     1441 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     1501 ctgaaggcgc agttttgcct ggcgggaaac cgcgccacca aagccttttg cgatcaaaac
     1561 ggcattcgct acgacaactg cggcaagatg ctggttgcca cgtccgatct cgaaatggaa
     1621 cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga atggcttaac
     1681 gccgacgaac tgcgcgagcg cgaaccgaat atcaccgggc tcggcggcat ttttgtgccg
     1741 tccagcggca ttgtcagcta ccgcgacgtg acggcggcga tggcaaaaat cttccagtcc
     1801 agaggcggtg aaattatcta taacgccgaa gtcagcgggc ttaatgagca taaaaacggc
     1861 gtggtgatac gtacccgtca gggcggcgaa tatgaagcat caacgctgat tagctgttcc
     1921 gggctgatgg ctgaccggct ggtgaaaatg ctcggactcg aaccgggctt tatcatctgc
     1981 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     2041 atttacccca ttcccgaccc ggcaatgccg tttttgggcg ttcatctcac ccgcatgatc
     2101 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     2161 aagcgcgact tctcatttag cgacacgctg gagattttgg gctcgtcggg gattcgccgg
     2221 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     2281 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     2341 tggcccgccg gtgtgcgggc gcaggcggta tcgccggacg gcaagctgat tgacgatttt
     2401 ctgtttgtca ccaccccgcg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     2461 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     2521 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     2581 aatcaataac ctttgaaaac aggatgtagc gatgaaactt aacgacagta acttattccg
     2641 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag ccatcgacgt
     2701 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cggatgaaac
     2761 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgcgcgc tcaccgccaa
     2821 agaacgcgcc accattctgc gcaactggtt caatttgatg atggagcatc aggacgattt
     2881 agcgcgcctg atgaccctcg aacagggtaa accactggcc gaagcgaaag gcgaaatcag
     2941 ctacgccgcc tcctttattg agtggtttgc cgaagaaggc aaacgcattt atggcgacac
     3001 cattcctggt catcaggccg ataaacgcct gattgttatc aagcagccga ttggcgtcac
     3061 cgcggctatc acgccgtgga acttcccggc ggcgatgatt acccgcaaag ccggtccggc
     3121 gctggcagca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     3181 ggcgctggcg gagctggcga tccgcgcggg cgttccggct ggggtattta acgtggtcac
     3241 cggttcggcg ggcgcggtcg gtaacgaact gaccagtaac ccgctggtgc gcaaactgtc
     3301 gtttaccggt tcgaccgaaa ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     3361 gaaagtgtcg ctggagctgg gcggtaacgc gccgtttatc gtctttgacg atgccgacct
     3421 cgacaaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     3481 ctgcgccaac cgcctgtatg tgcaggacgg cgtgtatgac cgttttgccg aaaaattgca
     3541 gcaggcagtg agcaaactgc acatcggcga cgggctggat aacggcgtca ccatcgggcc
     3601 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     3661 aggcgcgcgc gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc
     3721 gaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     3781 cctcgccccg ctgttccgct ttaaagatga agctgatgtg attgcgcaag ccaatgacac
     3841 cgagtttggc cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtggg
     3901 cgaagcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     3961 cccgttcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     4021 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaatgaaca
     4081 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     4141 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     4201 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     4261 tggccgtggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     4321 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     4381 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     4441 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgcgtatcac gggcgcacgc
     4501 attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     4561 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     4621 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     4681 tcgtgattga gccggttcag ggcgaaggcg gtttctacgc ctcgtcgcca gcctttatgc
     4741 agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     4801 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtt gcgccggatc
     4861 ttaccacctt tgcgaaatcg atcgcgggcg gcttcccgct ggcgggcgtc accgggcgcg
     4921 cggaagtaat ggatgccgtc gctccaggcg gtctgggcgg cacctatgcg ggtaacccga
     4981 ttgcctgcgt ggctgcgctg gaagtgttga aggtgtttga gcaggaaaat ctgctgcaaa
     5041 aagccaacga tctggggcag aagttgaaag acggattgct ggcgatagcc gaaaaacacc
     5101 cggagatcgg cgacgtacgc gggctggggg cgatgatcgc cattgagctg tttgaagacg
     5161 gcgatcacaa caagccggac gccaaactca ccgccgagat cgtggctcgc gcccgcgata
     5221 aaggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccgc
     5281 tcaccattga agacgctcag atccgtcagg gtctggagat catcagccag tgttttgatg
     5341 aggcgaagca gtagcgccgc tcctatgccg gaggcgacgc tgcgcgtctt gtccggccta
     5401 cggggatcca ggtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga
     5461 agaaatctat gttggccggg taaggcggag ccgctctccg gcaaaaagaa tcaataacaa
     5521 ttatacgcgt gacccggcgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     5581 gaggattcag gatggggcaa tcatcgcaac cacatgagtt aggcggcggg ctgaagtcac
     5641 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     5701 gcgtcgccat cgccgaagcg ggcccggcgg tattactggc ctatctgttc gccgggctac
     5761 tggtggttat gattatgcgg atgttggcgg aaatggcggt tgccacgccc gataccggtt
     5821 cgttttccac ctatgccgat aaagccattg gtcgctgggc gggctatacc atcggctggt
     5881 tgtactggtg gttttgggta ctggttatcc cgctggaagc caacatcgcc gccatgatcc
     5941 tgcactcatg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     6001 taactggcag taacttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     6061 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     6121 tttacccgta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     6181 ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     6241 cagaaattgt caccattgcc gccgcggaat ccgacacgcc ggaaaaacat attgtccgcg
     6301 ccaccaactc ggttatctgg cgtatttcta tcttctattt atgttctatt tttgtcgtcg
     6361 tggcattaat tccgtggaat atgcccggac taaaagccgt cggttcttat cgctcggtac
     6421 tggaattgct caatattccc catgcgaaat taatcatgga ctgcgtgata ttactttccg
     6481 taaccagttg cctgaactcg gcgctgtata ccgcgtcaag gatgctctac tccttaagtc
     6541 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg
     6601 cggtgttact ctccaccggc gcggcattct taacggtggt ggtgaactat tacgcgcctg
     6661 cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag
     6721 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     6781 gcttgcgaat gtggctttat ccgtggctca cctggctagt catcggcttt attacctttg
     6841 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
     6901 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
     6961 ggcaaaaaac acccgttcat aatacgcgct gatcatgatc aggagtcaca ccatgaccat
     7021 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
     7081 ggatgaaaaa ttacgaatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt
     7141 acgggaagct ctttcgcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
     7201 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
     7261 tatggaagcg atgttagtga gtctggcgat tgcccgcggt ggcgatgagt gggaggcaga
     7321 cgttctcgca aaagcgcatc tgctgagtaa gcttgaggcc tgtgacgcca gcgagaaaat
     7381 gcttgatgag tgggatctgc gtcatcaggc gtttcatacg gcaattgtgg cgggctgtgg
     7441 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
     7501 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
     7561 ccagaccctg actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg
     7621 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
     7681 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
     7741 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
     7801 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
     7861 ttttcacctg atcatcgaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
     7921 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
     7981 gaatattcac tttatcggca tccggtatac cggttttgtt cagatgctcc tgcaccttct
     8041 tcgcctgatc gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat
     8101 ctttcacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
     8161 tgtagcagag gaacgcggcg cgttaacgcc gcggggatga gcacaggttt aatcgcgggt
     8221 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca acagaatatt
     8281 aataatgaac gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat
     8341 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
     8401 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta
     8461 cgcttttact ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt
     8521 tacttaattt agaaaatact taaataaata tgactgaact cgcgcaatta caggccagtg
     8581 ccgaacaggc agcggcctta ttgaaagcaa tgagccaccc taaacgattg ctgattctgt
     8641 gcatgcttag cggttccccc ggcaccagcg cgggagagct gacgcgcatt accggactga
     8701 gtgcctctgc gacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc
     8761 aacgggatgc ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg
     8821 ccaccctgaa aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc
     8881 cgcatgatgc acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgatgctg
     8941 atgaatatct tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac
     9001 agtcaggtct tccggctaaa ttacgtcacg agcaaattat attccactgc caggcaggta
     9061 aacgcaccag taataatgcc gataaattag cagcgattgc cgcccccgca gaaatctttt
     9121 tactcgaaga tgggattgat ggctggaaaa aagcaggatt gccagtagcg gtaaataaat
     9181 ctcaaccctt gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg
     9241 gcgttgtact gggttatacc gtaaatagcg gtttcttctt attaagtggc tttgttggtg
     9301 ccgggttact gtttgcagga atcagcggtt tttgtggaat ggcaaggttg ttagataaga
     9361 tgccgtggaa ccaacgagct tgagaagcga cgccggacgc gccctagcag cgacatccgg
     9421 cctcagtaat tagatcagga aatcgtcgag agatttacct tctgccagcg cctgagcaat
     9481 tggcttcggt gtacggccct gaccggtcca ggttttagtt tcaccgttaa catcggtgaa
     9541 tttatatttc gccggacgcg gctggcgttt tttaccagcg cgtggtgcag cagcagagct
     9601 attacccaat aactcttccg ggttaattcc gtcagctttc atcagctcca gccaggtgct
     9661 aattttttcc tggcgctctg ccagttcacg ctgctgctgt tcttcttctt cacgtctttc
     9721 tttagtgaca accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg
     9781 agccatcgca cggagggtgc gaatgttatt taaactttgt aacattacgg acataataaa
     9841 gaaaaccttt taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta
     9901 ttatatttcc acaaaattta atctatttac agtcctgcgc gagattattt cagctgttat
     9961 tttctgaaac tctcagaata atttcaagat tatatattag ccattttgat gagtaattta
    10021 gattattggt gaacagagcg gcgctctctg ctcgctaatt ccgcaagcct gacaaaatca
    10081 aagtgaataa aaatgcaata taacagaata aaagaaaaca tccatcactg gtgctttctg
    10141 ctgttaatgt acattgtctg gcataaaaaa tgctgaaaat gacccagcta ctacatacac
    10201 cgaattctct acgcttatag cgttacctca cccccaaact ttcattagtg atattaaagg
    10261 taaggccata tctaattgat ttaattaaaa ataaatcata tcggcatgta aaaatccaca
    10321 atgtacaaaa aacgagccgt tacggaatat tttatctaca aaaactgact aaataaaaat
    10381 ttttcactaa ttgattagtc atagccagcg atatacgcta tgcgaaaatg cagatggcaa
    10441 tgagatccac tgctttcatc tccattaaca tcccattacg cttttattaa ggagcattag
    10501 catgttctca ccgcagtcac gcttgcgtca tgcagttgca gatacgttcg cgatggttgt
    10561 ttactgttct gtcgtgaaca tgtgtattga agttttcctc tccggaatga gcttcgaaca
    10621 gtctttttat tccagattgg tagcgattcc ggtgaacatc ttaattgcat ggccatacgg
    10681 tatgtaccgt gatctgttta tgcgcgcggc acgcaaagtt agcccgtcgg gctggataaa
    10741 aaatctggcg gatatcctgg cttatgtgac gttccagtca ccggtgtatg tggcgatctt
    10801 gttagtggtg ggcgcagact ggcatcagat tatggcggcg gtcagttcaa acatcgttgt
    10861 ttcgatgttg atgggggcgg tttatggcta cttcctcgat tattgccgcc gactgtttaa
    10921 agtcagccgt taccagcagg taaaagcctg actcttcttt tcgcgactgg catcgccagt
    10981 cgcgtcttta actggcttcg ccaaataaac cattcaaata acgttcaagc gcaacacgcg
    11041 aactaaagcc atgcgggata ccgtaatgct cgtgtcgctc gcctgctaaa tagagcggaa
    11101 attgctggta atgatcgcaa gttttaatat cggaagcggg ttcagccagt gtcgaactgc
    11161 gttcttttaa tgttggatga attaccagag cggtacgtcc cattcgcgct tcgcgattaa
    11221 catagacata attttcccca cggcggtaac catacgcttt ctgggttacc acatcgacag
    11281 taaaaccgac tttttcaagt acgcgcgcca cctcgtctgg tcgtaaatac atatttgatc
    11341 ctcgttatta tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
    11401 aagtccataa agccgcgatg aaccttagtt aagaaaatta tggtctacac tgaaaattac
    11461 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
    11521 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagcctcgaa tctgtattga
    11581 agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc acgcagtaaa gctcaggcat
    11641 tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg
    11701 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag
    11761 cagctgcggt aggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcaaaa
    11821 atgataataa atacgcgtct ttgaccccga agcctgtctt cggggtttct ttttgcctgg
    11881 tgaatcacaa aaatccccct accccgtcac gctcatatcc agggtaattt cgaccactat
    11941 ttgctatata ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
    12001 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacgga aatacgaatc
    12061 atgcgcatta ctatttacac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
    12121 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcggca
    12181 gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc
    12241 tggtctggtt tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt
    12301 gcatgagcca gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac
    12361 gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
    12421 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
    12481 gacaggtaat tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg
    12541 cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
    12601 aatgcggcgt gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa
    12661 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagga
    12721 aacgatggat taccacgcgc tgaatgcgat gcttaacctc tacgatagcg caggtcgcat
    12781 tcagttcgat aaagaccgcc aggccgttga cgcctttatt gcgacgcatg tgcgtccgaa
    12841 cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta
    12901 tgatgaaagc gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc
    12961 acacaccagc ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta
    13021 tacgttgaag acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtaacgat
    13081 ggtggcgctg acgctggcac aaggcgatga gacgctggcg ttgcaactga ccgatgaaat
    13141 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
    13201 cggcgaactg gtttcctgtt ttttgctgcg tattgaagac aatatggagt cgattggtcg
    13261 ggcggtaaat tccgcactgc agctgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
    13321 gaatctgcga gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat
    13381 tccggtgatg aaaatgctgg aagacgcatt ttcctatgcc aaccaactcg gcgctcgtca
    13441 gggggctggt gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgacac
    13501 gaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgcttg gcgtggtgat
    13561 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcctta
    13621 tgacgtagag cgagtttatg gcaagccgtt tgccgatgtg gccatcagcc aacactatga
    13681 cgaactggtt gccgatgaac gcattcgcaa aaaatacctc aacgcccgtg atttcttcca
    13741 gcgactggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacggt
    13801 aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt aatctctgct cagaaatttt
    13861 gcaggttaac agcgcctcag agtatgacga gaatctcgac tatacccgca caggccatga
    13921 tatttcctgc aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgattttgc
    13981 ccgcacggta gagactgccg tgcgcggttt aacggcagta tcagatatga gtcatatccg
    14041 cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat
    14101 gaatttacac ggctatctgg cgcgagaagg catcgcttat ggttcgccgg aagcactgga
    14161 tttcaccaat ctctatttct atgccatcac ctggcatgca ctgcgtacct cgatgttgct
    14221 ggcacgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
    14281 atattttagc caatatctgc aagggaactg gcagccgaaa acggcgaaag ttggcgaact
    14341 gtttacccgt agcggtatta cgttacctac ccgtgagatg tgggcgcagc tgcgcgacga
    14401 cgtgatgcgc tacggcatat acaaccagaa tcttcaggcg gtgccgccaa ccggttctat
    14461 ctcttatatc aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
    14521 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgagaatct
    14581 ggcgctgtat caggacgctt acgaaattgg cgcagaaaag atcatcgaca cctacgcgga
    14641 agcgactcgc catgtcgatc aggggctgtc gctgacgctt tttttccccg ataccgccac
    14701 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aagggtatca aaacgctcta
    14761 ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
    14821 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
    14881 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
    14941 aaaggtgccg ctgtcgaacg atattcctgc ctggcagaca ttaactgtcg tagaacaaca
    15001 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
    15061 cgcgccttct ctgatgcccg atgcactcac gcctcatgaa gaagcggtat tatcgaatat
    15121 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctatgcca
    15181 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
    15241 agctcagatt attcagcaac attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag
    15301 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
    15361 ccgcggaaag ctgaccaata ccgcggacct gatccgtctg attatccgcg atgaagcagt
    15421 ccacggttac tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca
    15481 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagtt
    15541 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
    15601 ctgttacaac gccaataagg ctttgatgaa tctgggctac gaaccgttat ttcccgcaga
    15661 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
    15721 cgatttcttt tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaaga
    15781 tgaagactgg aatttctgag ggtgttattt tcaaaaatat cactacccgc agcagggaaa
    15841 taattcccgc caaatagctt tttatcacgc aaataatttg tggtgatcta cactgatact
    15901 ctgttgcatt attcgcctga aaccacaata ttcaggcgtt ttttcgctat ctttgacaaa
    15961 aaatatcaac tttctcgatt tgctctcagc ccttatatca cgggaaattc cggcgatttg
    16021 ctcgcatcaa tattcatgcc acatttgcca tcaggggttg cctcagattc tcagtatgtt
    16081 agggtagaaa aaagtgacta tttccattgg gtaatatatc gacatagaca aataaaggaa
    16141 tctttctatt gcatggcaat taaattagaa attaaaaatc tttataaaat atttggcgag
    16201 catccacagc gagcgttcaa atatatcgaa caaggacttt caaaagaaca aattctggaa
    16261 aaaactgggc tatcgcttgg cgtaaaagac gccagtctgg ccattgaaga aggcgagata
    16321 tttgtcatca tgggattatc cggctcgggt aaatccacaa tggtacgcct tctcaatcgc
    16381 ctgattgaac ccacccgcgg gcaagtgctg attgatggtg tggatattgc caaaatatcc
    16441 gacgccgaac tccgtgaggt gcgcagaaaa aagattgcga tggtcttcca gtcctttgcc
    16501 ttaatgccgc atatgaccgt gctggacaat actgcgttcg gtatggaatt ggccggaatt
    16561 aatgccgaag aacgccggga aaaagccctt gatgcactgc gtcaggtcgg gctggaaaat
    16621 tatgcccaca gctacccgga tgaactctct ggcgggatgc gtcaacgtgt gggattagcc
    16681 cgcgcgttag cgattaatcc ggatatatta ttaatggacg aagccttctc ggcgctcgat
    16741 ccattaattc gcaccgagat gcaggatgag ctggtaaaat tacaggcgaa acatcagcgc
    16801 accattgtct ttatttccca cgatcttgat gaagccatgc gtattggcga ccgaattgcc
    16861 attatgcaaa atggtgaagt ggtacaggtc ggcacaccgg atgaaattct caataatccg
    16921 gcgaatgatt atgtccgtac cttcttccgt ggcgttgata ttagtcaggt attcagtgcg
    16981 aaagatattg cccgccggac accgaatggc ttaattcgta aaacccctgg cttcggccca
    17041 cgttcggcac tgaaattatt gcaggatgaa gatcgcgaat atggctacgt tatcgaacgc
    17101 ggtaataagt ttgtcggcgc agtctccatc gattcgctta aaaccgcgtt aacgcagcag
    17161 caaggtcttg atgcggcgct gattgatgcg ccgttagcag tcgatgcaca aacgcctctt
    17221 agcgagttgc tctctcatgt cggacaggca ccctgtgcgg tgcccgtggt cgacgaggac
    17281 caacagtatg tcggcatcat ttcgaaagga atgctgctgc gcgctttaga tcgtgagggg
    17341 gtaaataatg gctgatcaaa ataatccgtg ggataccacg ccagcggcgg acagtgccgc
    17401 gcaatccgca gacgcctggg gtacaccgac gactgcaccg actgacggcg gtggtgctga
    17461 ctggctgacc agtacgcctg cgccaaacgt cgagcatttt aatattctcg atccgttcca
    17521 taaaacgctg atcccgctcg acagttgggt cactgaaggg atcgactggg tcgttaccca
    17581 tttccgtccc gtcttccagg gcgtgcgcgt tccggttgat tatatcctca acggtttcca
    17641 gcaattgctg ctgggtatgc ccgcaccggt ggcgattatc gttttcgctc tcatcgcctg
    17701 gcagatttcc ggggtcggaa tgggtgtggc gacgctggtt tcgctgattg ccatcggcgc
    17761 aatcggtgcc tggtcgcagg caatggtgac tctggcgctg gtgttaaccg ccctgctgtt
    17821 ctgtatcgtc atcggtttgc cgttggggat atggctggcg agaagtccgc gagcggcgaa
    17881 aattattcgt ccactgcttg atgccatgca gaccacgcca gcgtttgttt atctggtgcc
    17941 aatcgtcatg ctatttggta tcggtaacgt gccgggcgtg gtggtgacga tcatctttgc
    18001 tctgccgccg attatccgtc tgaccattct ggggattaac caggttccgg cggatctgat
    18061 tgaagcctcg cgctcattcg gtgccagccc gcgccagatg ctgttcaaag ttcagttacc
    18121 gctggcgatg ccgaccatta tggcaggcgt taaccagacg ctgatgctgg ccctttctat
    18181 ggtggtcatc gcctcgatga ttgccgtcgg cgggttgggt cagatggtac ttcgcggtat
    18241 cggtcgtctg gatatggggc ttgccaccgt tggcggcgtc gggattgtga tcctcgccat
    18301 tatcctcgat cgtctgacgc aggccgttgg gcgcgactca cgcagtcgcg gcaaccgtcg
    18361 ctggtacacc actggccctg ttggtctgct gacccgccca ttcattaagt aactctgcac
    18421 ttgcccggtg acgccgggca ttatcaccct gccaaaaaaa ggaataacaa tgcgacatag
    18481 cgtacttttt gcgacagcgt ttgccacgct tatctctaca caaacttttg ctgccgatct
    18541 gccgggcaaa ggcattactg ttaatccagt tcagagcacc atcactgaag aaaccttcca
    18601 gacgctgctg gtcagtcgtg cgctggagaa attaggttat accgtcaaca aacccagcga
    18661 agtagattac aacgttggct acacctcgct tgcttccggc gatgcaacct tcaccgccgt
    18721 gaactggacg ccactgcatg acaacatgta cgaagctgcc ggtggcgata agaaatttta
    18781 tcgtgaaggg gtatttgtta acggcgcggc acagggttac ctgatcgata agaaaaccgc
    18841 cgaccagtac aaaatcacca acatcgcaca actgaaagat ccgaagatcg ccaaactgtt
    18901 cgataccaac ggcgacggaa aagcggattt aaccggttgt aaccctggct ggggctgcga
    18961 aggtgcgatc aaccaccagc ttgccgcgta tgaactgacc aacaccgtga cgcataatca
    19021 ggggaactac gcagcgatga tggccgacac catcagtcgc tacaaagagg gcaaaccggt
    19081 gttttattac acctggacgc cgtactgggt gagtaacgaa ctgaagccgg gcaaagatgt
    19141 cgtctggttg caggtgccgt tctccgcact gccgggcgat aaaaacgccg ataccaaact
    19201 gccgaatggt gcgaattatg gcttcccggt cagcaccatg catatcgttg ccaacaaagc
    19261 ctgggccgag aaaaacccgg cagcagcgaa actgtttgcc attatgcagt tgccagtggc
    19321 agatattaac gcccagaacg ccattatgca tgacggcaaa gcctcagaag gcgatattca
    19381 gggacacgtt gatggttgga tcaaagccca ccagcagcag ttcgatggct gggtgaatga
    19441 ggcgctggca gcgcagaagt aatttttatt cgggcggata aggcgttttt gccgcatccg
    19501 ccgttctgtg cacaatgcct gatgcgacgc tggcgcgtct tatcatgcct acaaaccaca
    19561 tcgcacattt caacaatcta tctttcatcc catattcatc aacatccgct attattgatt
    19621 tccagcttaa tcatcacctg atgaacaaaa ataatgacta aacctaatca tgagcttagc
    19681 ccggcgctga tcgtgctgat gtctatcgcc accggtctgg cggtagccag taactattac
    19741 gcccagccat tgctcgacac catcgcgcgt aacttttccc tttccgccag ttcggcaggc
    19801 tttattgtta ccgccgcgca gttgggctat gccgcaggtc tactgtttct tgttcccctc
    19861 ggtgatatgt ttgaacgccg ccgcctgatt gtctcgatga ccttactggc ggcatgcggc
    19921 atgttgatta ccgccagcag tcagtcgctg gcgatgatga tcctcggtac ggcattaacc
    19981 ggtttattct cagtcgtggc acaaattctg gttccgctgg cagcgacgct ggcttcaccg
    20041 gataaacgcg gcaaagtggt tggcactatt atgagcggtc tgctgttggg gatcttgctg
    20101 gcacggacag ttgccggatt gctggcgaat ctcggcggct ggcgcaccgt cttttgggtt
    20161 gcttcggtgt taatggcact gatggcgctg gcattatggc gtggtctgcc acaaatgaaa
    20221 tcagaaaccc acctcaacta cccacagttg ttgggttccg ttttcagtat gtttatcagc
    20281 gataaaattc tgcgtacccg cgcgttgctg ggctgcctga cctttgccaa tttcagcatt
    20341 ctctggacct caatggcctt tttgcttgcc gctccacctt ttaactacag cgatggtgtc
    20401 attggtctgt ttggacttgc gggagctgcc ggagcgttgg gcgctcgtcc ggcgggcggt
    20461 tttgccgata agggcaaatc gcaccacacc acaactttcg gtctgctgct gctattactt
    20521 tcatggctgg cgatctggtt tggtcacact tccgtactgg cgttgattat cggaatcctg
    20581 gtgctggatc tcaccgtgca gggcgtgcat atcactaacc agacggtaat ttatcggata
    20641 catcctgatg cgcgcaatcg cctgaccgca ggttacatga ctagctactt tattggcggt
    20701 gccgccggtt cgctaatttc agcctcagcc tggcaacatg gcggttgggc tggcgtttgt
    20761 ctggctggcg cgacgattgc cctggttaac ttactggtct ggtggcgagg ttttcatcgt
    20821 caggaagccg caaattaagc aatcagcaac cgttttcgtg gctttacact tataagggtg
    20881 ttaagaagcc catcagtctg ataaggttaa gatattcatt cagtctattt ataatattaa
    20941 caatcgttaa gcgtacactc tatggaaagc cctactccac agcctgctcc tggttcggcg
    21001 accttcatgg aaggatgcaa agacagttta ccgattgtta ttagttatat tccggtggcc
    21061 tttgcgttcg gtctgaatgc gacccgtctg ggattctctc ctctcgaaag cgtttttttc
    21121 tcctgcatca tttatgcagg cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg
    21181 agtagtttgt ggattgctgc actgaccgtc atggcaatgg atgttcgcca tgtgttgtat
    21241 ggcccgtcac tgcgtagccg tattattcag cgtctgcaaa aatcgaaaac cgccctgtgg
    21301 gcgtttggcc tgacggatga ggtttttgcc gccgcaaccg caaaactggt acgcaataat
    21361 cgccgctgga gcgagaactg gatgatcggc attgccttca gttcatggtc atcgtgggta
    21421 tttggtacgg taataggggc attctccggc agcggcttgc tgcaaggtta tcccgccgtt
    21481 gaagctgcat taggttttat gcttccggca ctctttatga gtttcctgct cgcctctttc
    21541 cagcgcaaac aatctctttg cgttaccgca gcgttagttg gtgcccttgc aggcgtaacg
    21601 ctattttcta ttcccgtcgc cattctggca ggcattgtct gtggctgcct cactgcgtta
    21661 atccaggcat tctggcaagg agcgcccgat gagctatgag gttctgctgc ttgggttact
    21721 agttggcgtg gcgaattatt gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc
    21781 ccgcccaacc aaacgtggcg cggtaggtat tttgctcgac accattggca tcgcctcgat
    21841 atgcgctctg ctggttgtct ctaccgcacc agaagtgatg cacgatacac gccgtttcgt
    21901 gcccacgctg gtcggcttcg cggtactggg tgccagtttc tataaaacac gcagcattat
    21961 catcccaaca ctgcttagtg cgctggccta tgggctcgcc tggaaagtga tggcgattat
    22021 ataacccaca agaatcattt ttctaaaaca atacatttac tttatttgtc actgtcgtta
    22081 ctatatcggc tgaaattaat gaggtcatac ccaaatggat agttcgttta cgcccattga
    22141 acaaatgcta aaatttcgcg ccagccgcca cgaagatttt ccttatcagg agatccttct
    22201 gactcgtctt tgcatgcaca tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa
    22261 ggctcagggg attaacgaga cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa
    22321 ccacagtatt cagccttctg aattaagttg tgctcttgga tcatcccgta ccaacgcgac
    22381 gcgtattgcc gatgaactgg aaaaacgcgg ttggatcgaa cgtcgtgaaa gcgataacga
    22441 tcgccgctgc ctgcatctgc aattaacgga aaaaggtcac gagtttttgc gcgaggtttt
    22501 accaccgcag cataactgcc tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga
    22561 tcagctcgag caaatcaccc gcaaattgct ctcccgtctc gaccagatgg aacaagacgg
    22621 tgtggttctc gaagcgatga gctaacgcgt catctcgctc aaaaatccag atttataaaa
    22681 gaaaaaatga ctggccagca tcgcaacatg ctggcctttt tggcaagcag gtcggctcag
    22741 ccgatgagtt aagaagatcg tggagaacaa tatgagcgca aatgcggaga ctcaaacccc
    22801 gcagcaaccg gtaaagaaga gcggcaaacg taagcgtctg ctcctccttc tcaccttgct
    22861 ctttataatt attgccgtag cgatagggat ttattggttt ttggtactgc gtcacttcga
    22921 agaaaccgat gacgcatacg tggcagggaa tcaaattcaa attatgtctc aggtgtctgg
    22981 cagcgtgacg aaagtctggg ccgataacac cgattttgta aaagaaggcg acgtgctggt
    23041 cactctcgac ccgacagatg ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc
    23101 cagcgttcgc caaacccacc agctgatgat taacagcaag cagttgcagg cgaatattga
    23161 ggtgcagaaa atcgccctcg cgaaagcaca aagcgactac aatcgccgtg tgccgctggg
    23221 caatgccaac ctgattggtc gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc
    23281 ccaggcgcaa ctggacgtcg cgattcaaca atacaatgcc aatcaggcga tgattctggg
    23341 gactaaactg gaagatcagc cagccgtgca acaggctgcc accgaagtac gtaacgcctg
    23401 gctggcgctg gagcgtactc gtattatcag tccgatgacc ggttatgtct cccgccgcgc
    23461 ggtacagcct ggggcgcaaa ttagcccaac gacgccgctg atggcggtcg ttccagccac
    23521 caatatgtgg gtggatgcca actttaaaga gacgcagatt gccaatatgc gtatcggtca
    23581 gccggtcact atcaccacgg atatttacgg cgatgatgtg aaatacaccg gtaaagtggt
    23641 tggtctggat atgggcacag gtagcgcgtt ctcactgctt ccagcgcaaa atgcgaccgg
    23701 taactggatc aaagtcgttc agcgtctgcc tgtgcgtatc gaactggacc agaaacagct
    23761 ggagcaatat ccgctgcgta tcggtttgtc cacgctggtg agcgtcaata ccactaaccg
    23821 tgacggtcag gtactggcaa ataaagtacg ttccactccg gtagcggtaa gcaccgcgcg
    23881 tgaaatcagc ctggcacctg tcaataaact gatcgacgat atcgtaaaag ctaacgctgg
    23941 ctaatccaga ggtgcgtgtg atgcaacagc aaaaaccgct ggaaggcgcg caactggtca
    24001 ttatgacgat tgcgctgtca ctggcgacat tcatgcaggt gctggactcc accattgcta
    24061 acgtggcgat ccccactatc gccgggaatc tgggctcatc gctcagccag ggaacgtggg
    24121 taatcacttc tttcggggtg gcgaatgcca tctcgatccc gcttaccggc tggctggcaa
    24181 agcgcgtcgg ggaagtgaaa ctgttccttt ggtccaccat cgcctttgct attgcgtcgt
    24241 gggcgtgtgg tgtctccagc agcctgaata tgctgatctt cttccgcgtg attcagggga
    24301 ttgtcgccgg gccgttgatc ccgctttcgc aaagtctatt gctgaataac tacccgccag
    24361 ccaaacgctc gatcgcgctg gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg
    24421 gcccgatcct cggcggttat atcagcgata attaccactg gggctggata ttcttcatca
    24481 acgtgccgat tggcgtggcg gtggtgttga tgacactgca aactctgcgc ggacgtgaaa
    24541 cccgcaccga acggcggcgg attgatgccg tggggctggc actgctggtt attggtatcg
    24601 gcagcctgca gattatgctc gaccgcggta aagagctgga ctggttttca tcacaggaaa
    24661 ttatcatcct taccgtggtg gcggtggtgg ctatctgctt cctgattgtc tgggagctga
    24721 ccgacgataa cccgatagtc gatctgtcgt tgtttaagtc gcgcaacttc accatcggct
    24781 gcttgtgtat cagcctcgcg tatatgctct acttcggcgc tattgttctg ctgccgcagt
    24841 tgttgcagga ggtctacggt tacacggcga cctgggcagg tttggcctct gcgccggtag
    24901 ggattattcc ggtgatcctg tcgccgatta tcggccgctt cgcgcataaa ctggatatgc
    24961 ggcggctggt aaccttcagc tttattatgt atgccgtctg cttctactgg cgtgcctata
    25021 cctttgaacc aggtatggat tttggcgcgt cggcctggcc gcagtttatc caggggtttg
    25081 cggtggcctg cttctttatg ccgctgacca ccattacgct gtctggtttg ccaccggaac
    25141 gactggcggc ggcatcgagc ctctctaact ttacgcgaac gctggcgggg tctatcggca
    25201 cgtcgataac cacgaccatg tggaccaacc gcgagtcgat gcaccatgcg cagttgactg
    25261 agtcggtaaa cccgttcaac ccgaatgccc aggcgatgta cagtcaactg gaagggcttg
    25321 ggatgacgca acagcaggca tcaggctgga ttgcccagca gatcaccaat caggggctga
    25381 ttatttccgc caatgagatc ttctggatgt cagccgggat attcctcgtc ctgctggggc
    25441 tggtgtggtt tgctaaaccg ccatttggcg caggtggcgg cggaggcggt gcgcactaag
    25501 tacaactaag ccagttcatt tgaactggct tttttcaatt aattgtgaag atagtttact
    25561 gactagatgt gcagttcctg caacttctct ttcggcagtg ccagttcttc gttgctgttg
    25621 atgcgtacgt cacgttccag aatgctacgc gcaatatcct gcgcttcctg caacgagtgc
    25681 atctggtaag tgccacactg gtagacgttc agttccggga tctgattctg atcctgcact
    25741 ttcagcacgt cttccattgc cgctttccag gcatcagcaa cacgctgctc atctggcgta
    25801 ccaatcagac tcatataaaa accggtgcgg cagcccattg gcgagatatc gataatctct
    25861 acaccattac cgttaagatg gttacgcata aaaccagcaa acaggtgctc cagggtatgg
    25921 atccctcttt ctggcatcac ttctttgttc ggcacgcaga agcgcagatc gaacacggtg
    25981 attgcgtcgc catgcggggt gttcattgtt ttcgccaccc gaactgcagg cgcttccatc
    26041 cgggtatgat cgactgtgaa gctatctaac aacggcattt agccacctcc ggtaattttt
    26101 ttaaaaattt tctgaactct ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt
    26161 tgttatcatc atccctgaat tcagagatga aattttggcc actcacgagt ggcctttttc
    26221 ttttctgtca ggcgtgtttt tccagccaca ccgcaaacgg ttcggtatca gcggcttcca
    26281 tttcctgctg acggcgttca gacgcctcgc gctcggctac aaaatcctct tcgcgcagaa
    26341 tttccagcgg ctcttcacgc agcagattac ggtaggcttc tgcaaatgct ttgcctgttc
    26401 cgccaatacc agtatcaatc atagacctta agatacgggc agagaaagtc agatcgggat
    26461 tatcgaagca ggcaaccagt tcatcacaca ctttctgata cgcttcgccg ccgttaatac
    26521 tatccagcgt ttgcgcgacg cgtttcagat cgcggaacag atctttaccc acctgcggta
    26581 acgggaactg tgcggtttcg cagccgatac ccagcgtcag acccggtttg cgaccttcga
    26641 ggatcacccg gttccagtta acgcgtgtac aggcaagttc gctactgctc atttccggtg
    26701 catcagccag cgcacaccag accataaaca ggtcgaggaa tcgcacctgc tgttcatcta
    26761 caccaatcgg cgagaacggg ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc
    26821 cacgtaacag cgcatcagaa ggcgactcgc cgctgcgggt aacgcgtttt ggacgaatcg
    26881 gcgcgtacag ttcgttttca atctgcaaca cgttgctgtt gatttgcagc ctcttaccgt
    26941 ctttctcaat accaatcttc gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc
    27001 ccgctacgta ctcgtaaaga tcgttgaagg taataccaag attgctttgc gatttattgg
    27061 tatagccgag atcgctcaaa cgaagagagg tcgcatacgg caggtaatac ataccgcact
    27121 cggttttctc aaacggcagc gacgttggtt ttccttgcag gaaagaagaa caaatcgccg
    27181 gagatgcacc aaacagataa ggaatgaccc aaccgaaacg atagtaattg cggataacgc
    27241 ggaaatagcc cgcagaaatt ttctctttgg catcagcgcc cgagatatca ccgcacttcg
    27301 cttgccagaa tgccattggc aaagagaaat tgtagtgcac gccggaaatg gtttgcatca
    27361 gcgcgccgta gcgatttttc agcccttcac gatacagcgt tttaaagcgt ccggtgttag
    27421 aagtgccgta ctgtgccagt tcgatgtcct gaccttctgc gatgtagcat ggcatactta
    27481 acggccacat ccgctcatcg cccatattgc gcgccgtata acgatgcaga tcgcgcataa
    27541 aggtcagcat atgttcaata tcaccatcca ctggtgtaat gaattccagc aatgcttccg
    27601 caaaatcggt agtaatccat ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg
    27661 ttgttgccag tgtgccatca gcattaacac gcaaagtttc gcgctccagc ccacgctgta
    27721 tcccctttaa cgcctgagga tgtttttcca gccaggccag cgcctgtgat acgtccggga
    27781 tcaaattgac ctcccgcctg tcaaaatcgt tttaattagc ataactgtaa tggtgaccat
    27841 atgtgcaggc ctacaattag tgccaccaca tcatgccctg aacggtcgct gctgcaactg
    27901 caacatagcg taacgcttta ccaaggcaca aaaaaaagat taccggtccc cacgagatgc
    27961 gcatccatcc cgctaacaga cacagtaaat cgccaaccac gggcatccag cttaataata
    28021 gcgtgactgc accatagcgt ttcagccagc cggtagcttt ctcttgccag cgcgatgttt
    28081 tacgcaatgg aaagaaacgc ccaaggataa cgttagttaa ccctccaagg ctattaccca
    28141 ttgttgctgt taaaactaaa acccagggat gactgatccc ggaaagcaac attgccacca
    28201 gcacgacttc ggagttgccg ggtaatagtg tagcgctgag aaaactactg gcaaacaacg
    28261 agaaaagcga taacgcttca ctcacagcaa gcgaacatcc acggcgtcca tgcctgctgc
    28321 acgggccgcc tgaataccga aatcggcatc ttcaaagacc acacactgcg tcggttgcac
    28381 gcccatacgc tgcgcgcaca acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg
    28441 atcggcagcg acgacggcgt caaaataatg gcgtaatccc aggtgcgcca gcaatgcctc
    28501 agcgatggcg ctttcactcc ccgttcctac agccattggg cgacgaccat gccaactttt
    28561 caccacatca acaagaggaa gcggttcgac gctatccagc agcatacttc ttactgcttc
    28621 tgttttttca cgcgctaacg catgcgggtc gagatcggcc tgattcagct caataattgc
    28681 ctgagcaata cgccaggtgg gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg
    28741 aagaccgtag tgccctaata cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag
    28801 gattgtgcca tccatatcaa aaattaaacc tgcataacgc tcgtacatgg tcttctcgca
    28861 agtcgaaaaa tatgacgcta ctttagcgta attgcttgat tttgtcgctg atggcgaagg
    28921 agaagaaaat tcaggaaatg gataaaggag ttatttagaa atgagatatt tttgaaggaa
    28981 attttttgga gaagatggtg catccgggag gatgactcac tgacgttcgc ccttcgggcc
    29041 gttgctggcg caacgttatc cttcctggtg cctgcggtta ataccgctat ctcttcgata
    29101 ccttcattgc tgaaggttac ggagaatatg gtgcatccgg gaggattcga acctccgacc
    29161 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaaacttat
    29221 tctactgctg atattgatac cgctactaaa gccatatcaa ttaagagatg gtgcatccgg
    29281 gaggattact cgccttgcgg ctcgcccttc gggccgttgc taaagcaacg ttatcatccc
    29341 tagtgcctgc ggttaattcc gctacctctt cgataccttc attgctgaag atttcggaga
    29401 atatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta
    29461 tccagctgag ctacggatgc atcgggaaat ttattttact gctgatattg ataccgctac
    29521 taaagccata tcaagtaaga gatggtgcat ccgggaggat tcgaacctcc gaccgctcgg
    29581 ttcgtagccg agtactctat ccagctgagc tacggatgca tcgggaaact tactttactg
    29641 cagatttttt gataccgcta ctaaagccgt atcaagtaag agatggtgca tccgggagga
    29701 ttactcgcct tgcggctcgc ccttcgggcc gttgctaaag caacgttatc ctccctggtg
    29761 cctgcggtta ttaccgccac ctcttccata ccttcatcgc tgaaagtacg tagaatatgg
    29821 tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc
    29881 tgagctacgg atgcaaatgg cggtgaggcg gggattcgaa ccccggatgc agcttttgac
    29941 cgcatactcc cttagcaggg gagcgccttc agcctctcgg ccacctcacc acacgcctct
    30001 tacgagtgct tcgaagaact tgtttatcgc tcatcgtcgc tgcgtggcgc acatattact
    30061 ttctgagact tataagtcaa acaatttttc ctacactttt atcgtttgca cacttcacgt
    30121 tcaattagtc tgtaaaaacg tcaaaaagag tgttttatca acagaagaat ggaggtctga
    30181 cagatagtag taatgcaaaa aaatggagac ttaagttgaa tgaacgggag taaagcgaaa
    30241 agactataga gtgaaggaga aattttgagg gtgcgtctca ccgataaaga tgagacgcgg
    30301 aaagattagt aactggactg ctgggatttt tcagcctgga tacgctggta gatctcttca
    30361 cggtgaacag aaacttcctt cggggcattt acgccaatac gtacctggtt gccctttacc
    30421 cctaaaactg tcacggtgac ctcatcccca atcatgaggg tctcaccaac tcgacgagtc
    30481 agaatcagca ttctttgctc cttgaaagat taaaagagtc gggtctctct gtatcccggc
    30541 attatccatc atataacgcc aaaaagtaag caatgacaaa cacattacat ctaagcagtc
    30601 atggcattac attctgttaa acctaagttt agccgatata cacaacttca acctgacttt
    30661 atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag tccacggcat tgcctgacgc
    30721 ttatattatt gcaatttcgc gctgacccag cctttcacac tggctaacgc tgcaggtaag
    30781 gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac gtccaccacc cttgccgccc
    30841 acctgctgag cgaccatacc aatcagttcc cctgctttca cacgatctgt gacgtcctta
    30901 gatacgcctg caatcagaga aaccttacct tcgactaccg ttgccagcac gataattgtc
    30961 gaccccagct gattttttaa atcgtcaacc atggtacgca acattttcgg ctcaacaccg
    31021 ctaagctcgc taaccaacag cttaacacca ttaacatcaa ttgccttact ggaaagattt
    31081 gcgctctcct gtgcggcagc ttgttcttta agctgttgta actctttttc cagctgacgc
    31141 gtacgttcca gtactgagcg cactttatca gccagattat tgctatcgcc tttcagcaga
    31201 tgcgcgactt cgcttaagcg atcgctgtct gcatgaacgg tggcgatagc accttctccg
    31261 gttaccgctt cgatacgacg aacgcctgca gcagtacccg attcagagat gatgcggaac
    31321 agaccaatat caccagtgcg gctggcgtga gtaccgccac acaactcggt agagaaatcg
    31381 cccatactca gcacgcgtac gcgctcatca tacttctcgc cgaacagcgc catcgcacct
    31441 ttcgctttcg ccgcttcgag atccatgatg ttggtttcga tcggcaaatt gcgacgaatc
    31501 tgtgtgttca ccaggtcttc gaccgcacga atctcttctg gtttcatcgc ttcgttgtgt
    31561 gagaagtcga agcgcagcac cttgtcgtta accagtgaac ctttctgcga tacatgagta
    31621 cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg cggagtgatt cagacgaata
    31681 cgggcgcgac gagcctcatc aacatcagcc tgcaccgcgt cgcccacttt cagagaaccc
    31741 gcagcaagtt taccgatgtg accaatcgcc tggccgtatt tctgcgtatc ttccaccgca
    31801 aaggagaagt tagcgccttt cagttcgcct ttatcgccaa cctgaccgcc ggattccgca
    31861 tagaatggcg tttgatccag cacgaccaca gcttcctggc ctgcattgat ggcatcaacc
    31921 gctttaccat caacaaacag cgcagtcact ttgccgttca gttccagatg gtcatagcct
    31981 ttaaattcag atgcactgtc aacacggatc attgcgttgt aatcggcacc aaagccgctg
    32041 gcttcgcgcg cgcgacgacg ctgctcttcc attgcagctt caaaaccagc ttcgtcaact
    32101 ttgatgttgc gctcacgaca aacatcagcc gtcaggtcaa ccgggaagcc ataggtgtcg
    32161 tacagacgga aagcagtttc accatccagc gtatcaccag aaagttttgc cagctcttca
    32221 tccagcaacg ccagaccgcg ctccagagta cgagcaaact gctcttcttc agtcttcagc
    32281 acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac ccgcagagcc cataacgtcg
    32341 atcagcggac caaccagttt gtagaagaag gtttctttcg cgccgagcat attaccgtga
    32401 cgcactgcgc gacgaatgat acgacgcagt acataaccac ggttttcatt ggacggcatt
    32461 acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt cagcgattac gcgcagcgat
    32521 ttattgctca gatcggttgc gccagtgact ttcgctaccg cctggatcag cgtgcggaac
    32581 aggtcgatgt catagttaga gttaacgtgt tgcagcaccg cagcaatacg ctccagaccc
    32641 ataccggtat ctacagacgg cttcggcagc ggttccatcg tgccatcggc ctggcggttg
    32701 aactgcatga agacgatgtt ccagatctca atgtagcggt cgccgtcttc ttccgggctt
    32761 cccggagggc ccccccaaat gtggtcgccg tgatcgtaga agatttcggt gcacgggccg
    32821 cacggaccag tgtcacccat ctgccagaag ttgtcagatg cgtatggcgc acctttgtta
    32881 tcgccgatgc gaataatacg ttcgcgcggg atccctactt ctttttccca gatttcgtag
    32941 gcttcgtcgt cgctttcata gacggtaacc cacagacgct ctttcggcag ggcaaaccat
    33001 ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat cgtgtttgaa atagtcgccg
    33061 aagctgaagt tgcccagcat ttcgaagaag gtatggtgac gcgcggtgta accgacgttt
    33121 tccaggtcgt tgtgtttacc acccgcacgc acgcagcgtt gggaagtggt agcgcgggaa
    33181 taattacgct tgtcgagccc aaggaacaca tccttgaact ggttcatccc ggcgttggta
    33241 aacaacaaag ttgggtcgtt atggggtacc agggagctgc tggcaactac ctgatgtccc
    33301 ttactatgga aaaagtcgag aaacgcctga cggatctcag cggtgctctt gctcataatt
    33361 atcctgaaat caagctaacg aaatatcgcc accagctcca gcgtgtctta accgccgggc
    33421 tggtaactga aaagtgggaa taagataagt tttcttgact gggaagtaaa ataccgtatg
    33481 cgttcagtcg gcaaaatttc gccaaatctc ctggatatct tccatcagat agccacgata
    33541 gagcagaaaa cgctggatct taactttttc tgaaaagaca gttggcaaag gttcgccata
    33601 ttttcgcgtc gcctgatcgc gcgccagtgc gcaccagtcg atgtcacatt cacgcatcgc
    33661 tttttctgtc gcttcgcggg aaataccttt ctgattcagt tcctggcgaa tacgcgcagg
    33721 tccataacct ttgcggctac ggctggcgat aaagcgcgca acaaatcggc tgtcatcgag
    33781 atagccatgt tcatggcacc aggcaataac gcgctcgtaa tcttctgccg tagcatcaat
    33841 ctcttctggg ccatttttgc ccataatcgg tgccgcgagt ttacgtcgca gttcttgctc
    33901 actgtgatcg cgcaccgcca gaatgcgtac cgcacgatcc aacaggcgag catatgccgg
    33961 gcgacgggat gttgattctg tcatggcata tccttacaac ttaaaaaagc aaaagggccg
    34021 cagatgcgac ccttgtgtat caaacaagac gattaaaaat cttcgttagt ttctgctacg
    34081 ccttcgctat catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca
    34141 cgtactttct tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagtcgca
    34201 ttcgctttac cctgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg
    34261 atcagcttct cttttacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg
    34321 tagaggatct ggaattcagc ctgtttaaac ggcgcagcga ttttgttctt caccactttc
    34381 acgcgggttt cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg
    34441 tcgagacgaa cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta
    34501 ccgaacatca caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac
    34561 tgcttcaggt tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc
    34621 atgtgagagt cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag
    34681 tcaacgacga taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc
    34741 tgctcgccgg tgtccggctg ggagcacagc aggttgtcga tatcgacgcc cagtttacgt
    34801 gcgtagattg ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc
    34861 tgcgctgcgg cgatcacctg cagcgtcagc gtggttttac cggaagattc cggtccgtag
    34921 atttcgacga tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc
    34981 gaaccggtag agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag
    35041 cctttaccaa attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt
    35101 tcgtcgatag ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct
    35161 gttgaagcaa ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca
    35221 caaggtctgc aatgcatacg cagtagcctg acgacgcacc gcatcacggt cgccgctgaa
    35281 gcattcccgc cgggtaatgc cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc
    35341 gacaggcttc tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcata
    35401 atcagcacga gccgctttca gtgcgcctat cgccatttcc accacgacgg gttcactcac
    35461 cgcgccatgc tgcgccagcg tctcttcgcg tacgccgatc atctgcgctt tggcttcgtt
    35521 actgtaggtg acaaatccgc gttcaaacca ggcggagcta ccggcaatat cggtaatcac
    35581 tttcgctacc caaccaccgg tacaagactc ggcagttgtt acggttgcgc cacgggcttt
    35641 cagcgcctgc ccaacctgtt cacttaactg catcagttca ctgtcagtca tcacaacctc
    35701 tgtcagtcaa aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg
    35761 ttgcaatttt acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc
    35821 ccctcttgta aatagctact gtactcgcgc cagcgccacg gcttgtccta actgccagac
    35881 cgccattgcg taatgggtgc tgtggttgta acgggtgatg gtgtagaagt tcggcagacc
    35941 gtaccagtac tggtagccgg tgccaacatc cagacgcagc aggctggctt gttgatggtt
    36001 gcccagcggc tgctgtggcg ttaaacctgc ggcggcaagc tgcgaaatgc tgtacttagt
    36061 tttgaagcca tttggcaagc ctggagcctg accgtttgcc attaccgcga cctgatcgcc
    36121 tttcacccag ccgtgcgctt tgaaatagtt cgccacgcta ccgatcgcat caaccggatc
    36181 ccacaggttg atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg
    36241 cataaactgt ccgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc
    36301 ctgctcgtcg cgcgccatca acaggaaggt ttccagctcg ccagagaaat actccgcgcg
    36361 gcgtgggtag ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac
    36421 gcgcccccag cgggtttcaa cgccgataat cccgacgata atttccggcg gtactccata
    36481 cacctgccac gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg
    36541 cacgttgtcc ggcgtaataa attttttgcg ataacggagc catgcgccgt tcggacctga
    36601 tggcggtttc accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag
    36661 acgcttcgcc tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac
    36721 cattttgtcg atgaactgct gggcattcgg gttattagcg aaatcgccgc ccatctgcat
    36781 cacattgtgc tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtatcagt
    36841 ctcagtaggt tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt
    36901 tacataacga cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg
    36961 gtaaagcatc acgccccgca caaggaagcg gtagtcactg cccgatacgg actttacata
    37021 actcaactca ttcccctcgc tatcctttta ttcaaacttt caaattaaaa tatttatctt
    37081 tcattttgcg atcaaaataa cacttttaaa tctttcaatc tgattagatt aggttgccgt
    37141 ttggtaataa aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt
    37201 gcagagtggt ttatcgggct gttccaaaag ggcggagagg tgtttaccgg gatggtgacc
    37261 ggcattcttc cgctgttgat tagcctgctg gttatcatga acgcactgat taattttatc
    37321 ggtcagcatc gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac
    37381 ctactgttac cgtgcattgg cacgtttgtc ttttgcaatc cgatgaccct aagcctgggt
    37441 cgctttatgc cggaaaagta caaacccagc tactacgcgg cggcctctta tagctgccac
    37501 tcaatgaatg gcctcttccc ccatatcaac cctggcgaac tgtttgttta tcttggcatt
    37561 gccagcggtc tgacaacgct gaacctgcca cttggcccac tggcggtgag ttatctgctg
    37621 gttggtctgg tcaccaattt cttccgcggc tgggtgaccg atctgaccac cgccattttt
    37681 gagaaaaaga tgggcattca acttgaacaa aaagttcacc tggcaggagc aacatcatga
    37741 cgcatattcg gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctgaaagcca
    37801 cgccgggaaa aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac
    37861 tggcacagct tactggctgg caggctattg acggatttaa agaaggtgaa cccgcggagg
    37921 cggaaattgg tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atctatccga
    37981 aacgacgtat tcccaccatt aatatccact cgacgggcaa gtccggtccg ctggcgcagt
    38041 acattgtgga agatatttat gtctctggcg taaaagaaga aaacatcact gtagtaggtg
    38101 atgcgacacc acaaccctct tccgtgggcc gtgactatga caccagtaag aaaatcaccg
    38161 aacaaagcga tggtttactg gcgaaggtgg gaatgggcat ggggtccacc gttgcggtgc
    38221 tgtttcaatc tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg
    38281 cattcgtctc ggcgctcatt ggcatcatta tggcttctgg ccttggtgac tggattgccc
    38341 acggtcttgc tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgtt
    38401 ccttcccact gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg
    38461 tattgattgg cgtgcagatt ggtctcggca atattccgcc gcatctggct ttaccggcac
    38521 tgtttgccat caacgcgcag gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg
    38581 aagcccgtca ggacacggtt cgcgtcggtg tcccttctgt actggtgagc cgctttttaa
    38641 ccggcgcgcc gactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc
    38701 tgaaacatga ccgttattta tcagaccacc atcacccgta tcggcgcgag tgccattgac
    38761 gccctcagtg accagatgct catcaccttt cgtgaaggcg cgcctgcgga cctcgaagag
    38821 tattgcttca ttcattgcca cggcgagttg aaaggtgcac tccatcccgg tttgcaattt
    38881 tcactcgggc agcatcgcta tccggtgacc gctgttggca gcgtggcgga agacaacctt
    38941 cgcgaactgg gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga atttccgggc
    39001 actgtccatg tggcaggccc tgtccccgac gatatcgcgc cgggatcggt tttgaagttt
    39061 gaatctgtta aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc
    39121 ttaggcgcgt tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat
    39181 attcagagcg acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaaagt
    39241 atggcgtacg gttttggtgc tgacgccact agcgagcaaa gcgttctggc gctctctcgt
    39301 ggggtagatg aaatctttgg tcgcgtggat ttgctggtct acagcgccgg aatagcaaaa
    39361 gcagccttta tcagcgactt ccagctcggc gattttgacc gttcgctaca ggtgaatctg
    39421 gtgggttatt tcctgtgtgc gcgtgaattt tcgcgtttga tgatccgcga cgggattcag
    39481 gggcgcatta ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc
    39541 tacagcgcag cgaaatttgg tggcgtcggg ctgactcaat cactggcgct ggatctggcg
    39601 gagtacggca ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc gccgatgttc
    39661 cagtcactgt tgccacaata cgcgaccaag ctgggtatca aaccggatca agtcgagcag
    39721 tattacatcg acaaagtacc gctcaaacgc ggctgcgatt atcaagatgt gctgaatatg
    39781 ctgctgttct acgccagtcc taaggcgtcg tactgcaccg gacagtcgat caatgtcacc
    39841 ggcggtcagg tgatgttctg atcaacagcg gagatccatt aaggatctcc gtgagactat
    39901 agaatgcctg atgcgctacg ctcatcaggc atacaggact tccgccacta cattaaggaa
    39961 aagttatggt atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct
    40021 taggcggctg gcaaatttct cgttttaacc gtgccttcga cacactatgc cagcaagggc
    40081 gggttggcgt gggccgttcc agcgggcgct ttaaaccgcg ggtcgtggtc gccatcgcgc
    40141 tggacgatca gcagcgcatc gtcgacacct tgtttatgaa aggactgacc gtcttcgccc
    40201 gaccgcaaaa aattcccgca attaccggta tgcatgcggg tgatttacag cccgatgtga
    40261 tctttcccca tgatccacta tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg
    40321 gataatttcg ttgtgaatgt tacttgcttg cgaagttatc attttgaaac ctaaatcagg
    40381 taatcacgcc catgaaacct cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc
    40441 agggtaaatg ctcggttgaa gaattggcgc aatactttga caccacaggc acaaccattc
    40501 gcaaagatct ggtcattctg gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg
    40561 tgttgaataa agaggaatcc gatccgccta tcgatcataa aacactcatc aacacccaca
    40621 agaaagagct gattgcagaa gctgccgtta gttttatcca tgatggcgat tcgatcattc
    40681 ttgatgctgg cagtaccgtt ttgcagatgg ttcccctgct ctcgcgcttt aataacatca
    40741 cggtgatgac caacagcctg catatcgtca atgcgctatc cgaactggat aacgaacaaa
    40801 ctatcctgat gccaggcgga acgtttcgca aaaaatcggc ctcatttcac gggcaactgg
    40861 cagagaatgc cttcgagcat ttcaccttcg ataaattgtt tatgggcacc gacggcatcg
    40921 atctcaatgc gggcgtaacc acctttaacg aggtttatac cgtcagtaag gcaatgtgca
    40981 atgccgcgcg cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt aaaagcccca
    41041 acgtagtttg cagtcttgaa agcgtcgata agctgattac cgacgcaggt atcgatccgg
    41101 cgtttcgtca ggcgctggaa gagaaaggga tcgatgtgat cataaccgga gagagcaatg
    41161 agtgaagcac tactgaacgc gggacgtcag acgttaatgc tggagttgca ggaagcaagc
    41221 cgtttaccgg aacgtctggg cgatgatttt gttcgcgccg ccaatatcat cctgcactgt
    41281 gaaggcaaag tggtggtttc gggaattggc aaatcgggcc acattggtaa gaaaatcgcc
    41341 gcaacgcttg ccagtaccgg cactccggct ttttttgtcc atccggcaga agcgctgcac
    41401 ggcgatctgg ggatgatcga aagccgcgat gtgatgctgt ttatctctta ctccggtggc
    41461 gcgaaggaac tggatctgat tattccgcgt ctggaagata aatctatcgc gctgctggcg
    41521 atgaccggca aaccgacgtc accgctgggc ctggcggcaa aagcggtgct ggatatctcc
    41581 gtagaacgcg aagcctgccc gatgcacctt gcgccgacct ccagcaccgt caataccctg
    41641 atgatgggtg acgcgctggc gatggcggtc atgcaggcgc gcggatttaa tgaagaagat
    41701 tttgcccgct cccacccagc cggggcactg ggcgctcgct tgctgaataa agtgcatcat
    41761 ctgatgcgcc gtgacgatgc catcccacag gtggcgttaa ccgccagcgt gatggatgcg
    41821 atgctggaac tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag
    41881 gtacaaggcg tctttaccga cggcgattta cgtcgctggc tggttggcgg cggcgcactc
    41941 accacgccag tcaatgaagc gatgacggtc ggcggcacca cgttgcaatc gcaaagtcgc
    42001 gccatcgacg ccaaagagat cctgatgaag cgcaaaatca ctgccgcacc ggtggtggat
    42061 gaaaacggca aactcaccgg cgcaataaac ctgcaggatt tctatcaggc cgggattatt
    42121 taatccttca atcccagacg tttcgccagc cgatgcaggt tggcgacgtc ggtttccagc
    42181 atccgcgcgc aggcagccca gttgtgatga ttttgtgcca gtgcctgacg aatagtttca
    42241 cgctggaacg cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac cgccgccact
    42301 tctggcgtcg gcaacgtcac ctcaggaaaa gcaaaatgtt gcgcctcaag aatcacttca
    42361 tcgccgctgc gggtggctct cgccagaact accgcccgat gaatagcatg ttccagttcg
    42421 cgcacgtttc ccggaaaact gtagtgttgc agtaaatttc gcgctccggc acttaatacc
    42481 acgcgggaga gcccctgccg caaacgacac tgctcgcaga aataccccgc cagcagaatg
    42541 acatcatcgc cccgctcacg cagcggcggc accgaaagtg gaaacacgct caggcgatga
    42601 aacaaatcgg cgcggaatcg ccctgccagc acctcttcgc gtaaatcgcg gttagtcgcc
    42661 gccagcacgc gcacatcgac ccgcaaacaa cggtcatcgc caacgcgctg aatatcgcca
    42721 tactgcaaca ccctcagcag cttggcctgc aatgccaacg acaactcgcc gatctcatcc
    42781 agaaacagcg tgccgttatc cgccatttca aacttcccgc tgcgattact gatagcgcca
    42841 gtaaacgctc ctttcacatg cccgaacaac tcactttccg ccacactttc cggcagtgca
    42901 gcacagttga gatagaccag cggattcacc gcccgtggcg aggcttcatg aatcgctttc
    42961 gccaccagct ccttaccggt tccagtctca ccgctgatca ggacgttgag atcggacgcc
    43021 gccacaatct caatctcttt tttcagttgc gtcatgccag gggacaagcc aatcatctgc
    43081 gtctgtttca ccgcttcaaa cggcgtggca tcgcctggca gcatattctg gctttccagt
    43141 tgttcaatca gcaacgcatt gcttaacgct cccgccgcca gcgcagcaat cagccgtagc
    43201 tcttcgtcgc tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt cagtgcgccg
    43261 atcaggtttt gcccggcaaa caatggcaga ccaacgcagg cgtgaacctt cagactctcc
    43321 tgcccaggaa tcaaaccgtc atagggatcg ggcaattcgc tgtctgcggg aaagcgcacc
    43381 acatccccgg cgcgggcaat cgcttccagc cgtggatgcc cttccagcgc aaagcgtcta
    43441 ccgagtacat cctttgccag accgtcgatg gcaagcggaa taaactgccg cgaatcgtaa
    43501 cgtagcaacg cagacgcatc gcactccagc acctgacgta gcgtggtgat caggcgctga
    43561 aaacgatcct ggtgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca
    43621 acggaaaaac tcatctttgc ctcactgtca atttgactat agatattgtc atatcgacca
    43681 tttgattgat agtcattttg actactcatt aatgggcata attttattta tagagtaaaa
    43741 acaatcagat aaaaaactgg cacgcaatct gcaattagca agacatcttt ttagaacacg
    43801 ctgaataaat tgaggttgct atgtctattg tggtgaaaaa taacattcat tgggttggtc
    43861 aacgtgactg ggaagtgcgt gattttcacg gcacggaata taaaacgctg cgcggcagca
    43921 gctacaatag ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata
    43981 aattcagccg cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg gatatcgatt
    44041 acatcgtgat taaccatgca gaagaggacc acgctggggc gctgaccgaa ctgatggcac
    44101 aaattcccga tacgccgatc tactgtactg ccaacgctat cgactcgata aatggtcatc
    44161 accatcatcc ggagtggaat tttaatgtgg tgaaaactgg cgacacgctg gatatcggca
    44221 acggcaaaca gctcattttt gtcgaaacac caatgctgca ctggccggac agcatgatga
    44281 cttacctgac aggcgacgcg gtgctgttca gtaacgatgc tttcggtcag cactactgtg
    44341 acgagcatct gttcaacgat gaagtggatc agacggagct tttcgagcag tgccagcgtt
    44401 actacgccaa tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt accgagatcc
    44461 tgggctttaa cttaccagtc gatatgatag cgacctccca cggcgtggtc tggcgcgata
    44521 acccgacgca aattgtcgag ctgtacctga aatgggcggc tgattatcag gaagacagaa
    44581 tcaccatttt ctacgacacc atgtcgaata acacccgcat gatggctgac gctatcgccc
    44641 aggggattgc ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg
    44701 ataaaaacga aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga
    44761 cgatgaataa cgtgatgatg ccgaaaatcg ccgggctggt ggaggagatg actggtttac
    44821 gcttccgtaa caaacgcgcc agtgctttcg gctctcacgg ctggagcggc ggtgcggtgg
    44881 atcgtctttc cacgcgcctg caggatgcgg gtttcgaaat gtcgctcagc ctgaaagcca
    44941 aatggcgacc agaccaggac gctctggagt tatgccgtga acacggtcgc gaaatcgccc
    45001 gtcagtgggc gctcgcgccg ctgccgcaga gcacggtgaa tacggtagtt aaagaagaaa
    45061 cctctgccgc cacgacggct gacctcggcc cacggatgca gtgcagcgtc tgccggtgga
    45121 tttacgatcc ggcaaaaggc gagccaatgc aggacgttgc accaggaacg ccgtggagtg
    45181 aagtcccgga taacttcctg tgcccggaat gctccctcgg caaagacgtc tttgacgaac
    45241 tggcatcgga ggcaaaatga gcaacggcat tgtgatcatt ggttcgggct tcgccgcccg
    45301 acaactggtg aaaaatattc gcaaacagga cgccactatt ccattaacct tgattgccgc
    45361 cgacagcata gatgagtaca acaaacctga cctcagccat gttatcagtc aggggcaacg
    45421 tgccgacgac cttacccgcc agacggcggg tgaatttgcc gagcagttta atctgcgcct
    45481 gtttccgcac acctgggtga cggatatcga tgccgaagcc catgtggtga aaagccagaa
    45541 taatcagtgg caatacgaca agttagtgct ggcaaccggt gccagcgcct ttgtcccgcc
    45601 tgtgcctggg cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga
    45661 aacgcaactg cgggatgccc gacgcgtgtt gattgttggc ggtggtttga ttggtagcga
    45721 actggcgatg gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag
    45781 tattctggcg tcgttaatgc caccggaagt aagcagccgc ttgcagcatc ggttgacgga
    45841 gatgggtgtt catctgctgt tgaaatctca gttgcagggg ctggaaaaaa cggattctgg
    45901 cattctggca acgctggacc gccagcgtag catcgaagtg gatgcggtaa ttgccgccac
    45961 cggactgcgc ccggaaaccg ccctggcacg acgcgccggg ctgacgatta atcgcggcgt
    46021 ttgcgtcgat agttatctgc aaaccagtaa taccgatatt tacgcgctgg gcgattgcgc
    46081 ggaaattaac ggtcaggtat tgccgttcct ccagccgatt caacttagcg cgatggtgct
    46141 ggcaaaaaat cttctcggca ataacacgcc gctgaaactc ccggcgatgc tggtgaaaat
    46201 caaaacgccg gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg
    46261 gcaaattaat accgaacgcc agggaatggt ggcgcgcggc gttgacgatg ctgaccagct
    46321 tcgcgccttt gtggtcagtg aggatcggat gaaagaggca tttggattgt tgaaaacatt
    46381 gccgatgtag gtgggctact gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc
    46441 gacctacggg gacgcatgtg taggccggat aaggcgttta cgccgcatcc ggcaatggtg
    46501 tccaaatgca acacgtttta tccgttctgg acttcacccg ctaaccaacg cgccgcagca
    46561 ataaccccct gccccagaga caaaccgcca tcacccgccg gtaaactctg tggaaagagc
    46621 aatgtgaaat cagcgagata atgcgccaga cgtgcacgca gcaaacggtt atgaataacc
    46681 ccgccgctaa ataccagcgt agtgatacca cgcatcgtgg cctgctcacg catcaacgcg
    46741 gcaaaaccct gcgccagcgc atcatgaaac gcccacgcgc gttgattaac cggtgcctgc
    46801 cagttcagcc actgctgcca gaaagtggcg agatccagtt gattgtccac ccgcggcatt
    46861 gtcaccggat gcgtcactcc gtggcatgag gctgcgagcg cctccagagc acaagccgct
    46921 tcaccttcat aacttaacgt ggctggcgca cagcccagtg ccgccgccac tgcatcgaaa
    46981 aaacgcccac acgatgacgc cagcggcgcg ttaattccac gctcaatggc ccgcgccagc
    47041 acgctccagt tttgctgttg cacacttgct gtttcagagt aattctgcca ctccggcaca
    47101 aagcgcaggc actgcgccag caggtttcgc cacggctgct tcgctgccaa atcgccaccc
    47161 ggaagcgcca ctgcaggcaa gccgcccagg tgctcacatt cgcgatagtt cacccgcagg
    47221 cactcgccgc cccacaaagc gccgttctcc cccataccga taccgtcgag cgtcaaagca
    47281 atgacatcac cgccatccag cggccactga tgctctgcca gacacgccgc tgcatgggca
    47341 tgatgatgca gtaccgtttg cgtcggcaga ttcatttcac gcgcccactg gctggagaca
    47401 tagcccggat gcgcgtcatg cacaacgtat tgcggggtaa aatcgtagat gttttgcatc
    47461 aggcgtaacg cttcgcgcca ctgcatctgg atgccatcgt cacttaaatc gcccagatgc
    47521 tgactcaaca ccgcttgttc accgcgcacc aggcagaagg tatttttcag atccgcgccg
    47581 agacacagca caggcggaac atttttaaag cccggaggca aagccagcgc atccggcaca
    47641 tacccccgcg aacggcgcag catttcgccg ctttcgcgca ccaccgaatc atccatccgc
    47701 tgcacgatgt cgcggttatg tatcaagaat ccgtcggcaa tgccctgcaa atccgccagc
    47761 gcctgttcgt tgctgatagc tggtggttta ccgctcaggt tgccggaggt catcaccagc
    47821 gggcattgca gttcctgtaa cagcaaatgc tggagcgggt tcgcaggcaa cattaccccg
    47881 acttcgttaa ggtcaggggc gatatcatca caaagctcag gaacgtattt tttatccacc
    47941 agcacaatcg gcgcggcggg cgtggtaagc aactggcgcg cagcgtctgg taaaccgtca
    48001 gccactggca acatgaccgc cagcggtttc gccgggcgat gtttgcgcgc ccgaagtgtc
    48061 gccaccgcgt tactgttacg tgcatcgcag gcaagatgaa atccgccaat ccctttgatg
    48121 gcgacaattt tgcccatttt taactgtgcg atagctgcct gtaatgccgc ctcttgttcc
    48181 gcatgttcac catgacttac ccattcaaga tgcgggccac actccgggca ggccaccggc
    48241 tgggcgtgga agcgacgatc gagcgggtca cggtactctt tgtcacaggc cggacatagc
    48301 ggaaacgccg ccatcacggt aaacgggcgg tcgtaaggca tggcgcgaat aatggtgaaa
    48361 cgcgggccgc agtgggtaca gttgataaac ggataacgat aacgccgttc gcctggggta
    48421 ttcatttcgg caaggcaagc agggcaagta gcggcatcgg gaacaatttg cgtattcatg
    48481 gtgccgcctg tgctctggcg tatagtgaac tcggtgggca gttgtgacca gataaacggc
    48541 tcacgctcga cgctatcaat acgcgccagc ggcgggcagt gctgatacaa ttgaacaaga
    48601 aacgtttccg ggtcttcccg cagccggact tctacgccat cgccgtcatt acagacatcg
    48661 ccgtgaagat ttaattgctg tgccagctgc cagacaaacg gacgaaaacc gacgccctgc
    48721 actttgccac gaatacgcag ttggacaccg caagatgtgt tttttgccat tgagttattc
    48781 ccgccatcat gaattgcgta acccgccctg ccggacacga cagcgtcgca tccggcagtc
    48841 acaggtcggc gataccgccc gctccgtatt ctacgaatat ttccgggaat tcctttgatg
    48901 ccagaacagt tctgtaagat ttttagaaca tcagcgccgt acggcggcgt ttttctgcgc
    48961 tcagttgttc aagtttatta cgatcgacac aaatcagcgc atgagtcggg caagccgcca
    49021 tacacgccgg gccgtcttca cgatggttgc acaggtcgca tttattggct tcggctttgt
    49081 cagcccgtac attcagaccc gcgccgctgt tgcggatcac cggacgtacc accacttcca
    49141 tcgcaccata cgggcaagcc acaacgcagg ttttgcaacc aatgcaacgt tcctgcatca
    49201 catgaacaaa ccctttatca cggctgatag caccattcgg gcagacgtta gcgcacggtg
    49261 catcttcaca ctgacggcaa actgtcgccg tggaaatgtt cacaccttta atgacatgga
    49321 tacgcggtaa aaaagtttcc ggggtcagcg atgcacagtc ctgattttcc tgatgagaaa
    49381 ccacgcacgc tacttcacag gtacggcaac caatacattt actcgcgtca gcaatgatga
    49441 aacggttcat caaattctcc agcaatgaca gttaatgcgc cgatacattc acaaatcatg
    49501 ccagttttta atttactgtt atttaaggaa attaatttct gtaatgcagg aaaaacgatg
    49561 tcatcgacac tagtgacgat gacatgtgat gacaatgttt atcgcgaagg agcaatgagt
    49621 gagtcgcggc ggatcagttt tccgctgaag gttttcggcg gtgagaaatc cccgccatcg
    49681 agcataaaaa tcagccgtcc aataatttcc tgaatcatct cagtcaccgg aatttttacg
    49741 ctggagagcg ccggaacggt gtagggggca atagcgatat catcgaatcc gataactgac
    49801 acctgctctg gcaccgctac gccgcgctcg tgtaacgctt tcatcgcacc tatcgccata
    49861 tcgtcgttac tggcaactaa cgcgctaaat ttagccccac gttcgagcaa catttctacc
    49921 ccttcggccc cgctggcagg cgtccattta ccgttagcga taagtttttc attgagcgca
    49981 ataccatgct gcgccagcgc gtctttatac ccggcaagac gttcaatgct ggtgggggaa
    50041 tccatcgagc cggtaaggaa agcaatctcc tgatgcccgg cgtttatcaa ctctgccacg
    50101 gcgttaaaac tggtctgttt atgatcgcac cagacgctat ggctgctgtt tttgcgcagg
    50161 cggcgattaa gcaccattat cggctgactg tgcgcgtcaa tgatgtcatc gatctcatcc
    50221 acgcttaaaa aacgcgggta aatcatgatc gcgtcgcagc gcagatccag cagatactga
    50281 atcgcctggc gctcttcttc tgcgctgtgt ttaccatctg ccaatagcaa ctgccgccct
    50341 ttctcttccg ccattcgcgc ggcatgaaag agtaattcac taaaataaat gccgtggtaa
    50401 agcgtgttgg tcactaccag ccccagcgtc tgagtactct tcgccgacag attgcgcgcc
    50461 agcaagtttg gacggtaacc gctctcttct accgcctgaa acacgcgatc tttagtctcc
    50521 tggctgacgt agccattacc tgaaagcacg cgggaaacgg tcgcttttga aaccccggcg
    50581 cgcttcgcca cttccagcat cgtcgtcatc attttcatcc ctttacacgc aatcaacgca
    50641 gtgtactgca ccgtttgccg attgtccttg cacaatcggc gggaaaaata ttcaggtgac
    50701 cggtttcaca aatataaaaa atgaacaatt cactctcttg cttatttagt gacaactatt
    50761 catgattttg tgaaaccggt ttcttaattc cgtttcagca tcggcatttt tccgtcacgt
    50821 cgactgataa caactacatc taccctactg ataacaggat aaaatccgat ggccaaaaat
    50881 tatgcggcgc tggcacgctc ggtgatagcg gcactgggcg gcgttgataa catctcggcg
    50941 gtcacgcact gtatgacgcg gttgcgcttt gttatcaaag atgatgcact tatcgacagc
    51001 ccgacgttaa aaaccatccc cggcgtgctc ggcgtggtac gtagtgacaa ccagtgtcag
    51061 gtgattatcg gcaataccgt ttcacaagcc tttcaggaag tcgtcagcct gctgccggga
    51121 gatatgcagc ccgcacagcc cgtgggtaaa cccaaactca cgctacgtcg cattggtgcg
    51181 gggatcctcg atgcgctgat cggcaccatg tcaccgctga tcccggcgat tatcggcgga
    51241 tcgatggtca aactgctggc aatgatcctc gagatgagcg gcgtgctgac aaaaggatcg
    51301 ccgaccttaa ccattctgaa tgtgattggt gacggtgctt tcttcttcct gccgctgatg
    51361 gtcgcggcat ctgccgccat caaatttaaa accaatatgt cgctggcgat tgccatcgcg
    51421 ggtgtgctgg tacatccgag ttttattgaa ctgatggcga aagcggccca gggtgaacat
    51481 gtcgaatttg ccctgattcc ggtcaccgcg gtgaaataca cctacacggt gatcccggcg
    51541 ctggtgatga cctggtgcct gtcatatatc gaacgctggg tggacagcat tacaccggcg
    51601 gtgaccaaaa acttcctcaa accaatgctg attgtgttga ttgccgcacc gctggcaatc
    51661 ctgctgattg gcccgattgg tatctggatc ggtagcgcca tttcggcgct ggtttacacc
    51721 attcatggtt atctgggctg gctttcagtc gccattatgg gcgcgctgtg gcctctgctg
    51781 gtaatgaccg ggatgcaccg cgtctttacg ccaaccatca ttcagaccat tgccgaaacc
    51841 ggcaaagaag ggatggtcat gccgtcagag atcggcgcta acctgtcgct gggcggttca
    51901 tcactggcag tggcgtggaa aacgaaaacc ccggaactgc gtcagacggc gctggctgcg
    51961 gcggcatcag ccattatggc ggggatttcc gaaccggcgt tatatggcgt ggcgatccgc
    52021 ctgaaacgtc cgcttatcgc cagtcttatc agcggtttta tttgcggcgc ggttgccggt
    52081 atggcggggc ttgccagcca ctcaatggca gcgccggggc tatttaccag cgtgcagttc
    52141 ttcgatccgg cgaatccaat gagcatcgtc tgggtgttcg cggtcatggc gctggcggtg
    52201 gtgctgtcgt ttatcctcac actgttgctc ggctttgagg atattcctgt tgaggaagcg
    52261 gctgcccagg cgcgaaagta tcagagcgta caaccgaccg tcgccaaaga agtaagtctt
    52321 aattgaggat gaaaatgtca gtatttccag aaagtttttt atggggcggc gcgcttgccg
    52381 ccaaccagtc tgaaggtgcg ttccgtgaag gtgacaaagg tctgaccact gtcgatatga
    52441 tcccacacgg cgagcatcga atggcggtga aactggggct ggaaaaacgt tttcagttgc
    52501 gagatgacga gttttatccc agccatgagg cgacggattt ttatcatcgt tataaagaag
    52561 atatcgccct gatggcagag atgggattca aggttttccg tacctcaatt gcctggagcc
    52621 gtctctttcc gcagggcgat gaaatcacgc ccaatcagca gggcattgct ttttatcgtt
    52681 ctgtctttga agagtgtaaa aagtacggta tcgaaccgct ggtcacgttg tgccacttcg
    52741 atgtgccgat gcatctggtc accgaatatg gctcctggcg taaccgcaag ctggtggagt
    52801 ttttcagccg ctacgccaga acctgctttg aagcatttga tggtctggtg aaatactggc
    52861 taaccttcaa tgaaatcaac attatgttgc atagcccgtt ctccggcgcg ggtctggtgt
    52921 ttgaagaagg tgaaaatcag gatcaggtga aatatcaggc cgcgcatcac cagctggttg
    52981 ccagtgcgct agccaccaaa atcgcccatg aggttaaccc gcaaaatcag gtgggctgta
    53041 tgctggcggg cggtaacttc tacccttaca gttgcaagcc ggaagatgtc tgggcggcgc
    53101 tggagaaaga ccgggaaaac ctgtttttta tcgatgtgca ggcgcggggc acgtatccgg
    53161 cttactctgc ccgcgtattc cgcgaaaaag gggtaaccat caacaaagca ccgggcgatg
    53221 atgaaatcct gaaaaacacc gtcgattttg tctctttcag ctattacgcc tcgcgctgcg
    53281 cctcggcgga gatgaacgcc aacaacagca gtgcggcgaa cgtggtgaaa tcgctgcgta
    53341 atccgtatct acaggtgagc gactggggct ggggaattga tccactcggt ctgcgtatca
    53401 ccatgaatat gatgtacgat cgttatcaga agccgctgtt tctggtggaa aacggcctgg
    53461 gcgcaaaaga tgaatttgct gccaatggcg agattaacga cgactatcgc atcagctact
    53521 tacgcgaaca tatccgcgca atgggcgaag cgattgcaga cggcattccg ctgatgggct
    53581 acaccacatg gggctgtatt gatttagttt ccgcctctac gggtgaaatg agcaaacgct
    53641 acggctttgt ctttgttgac cgtgacgacg caggcaacgg tacgctgacg cgcacgcgta
    53701 agaaatcatt ctggtggtat aaaaaagtga ttgccagtaa tggggaagat ttagagtagg
    53761 taacagtgcc ggatgcggcg tgaacgcctt atccggccta catgtcgcac gaaccatgaa
    53821 ggcccgataa gcgcagcgca tcgggcaatt tagcatttgg catcagagct ggttcacccc
    53881 atcaagaaca tccctgtcct gattccttaa tgaaaaacta ctcttcttcc accgctaact
    53941 gcgcgaagcc gccatttcct tcccagcctt ccagtcgttg ataaacggtt tctaccgcat
    54001 ctttaatcgg ctgggtcatc gggtagtaaa agccgacgat atccggctga atgccgagga
    54061 aaatcacttc gccaatatct tctttcaact ggtcgataag gtaattcaac ggcatgttat
    54121 gggtagtcat cataaacatc tcggcgatat catccgggtc gatgatgcgg atctcgccgg
    54181 ggtttagccc catatccgtg gcgtcgacaa tcagcagtcg tgtcgggcgc agttcacgga
    54241 tagcgacgat gtcgttttcc ggtgcgctac cgccgtcaat caccacccag ttacctttcg
    54301 gcgcggcggc gcacttttcc gccagcagcg gacctgcgcc atcatcgccc atcatgctat
    54361 tgccaacaca gagtaaaacg tcagtcacgc agtctcctca ccatcaaata gatggcgttt
    54421 tcctgatgaa tatcatgcag catacttaac agcattgtac tccattcctg ctgctgcgaa
    54481 gtttgcgctg cccgggcttt atcgaatgcg ttggccagca tcggcacatg gttaatgtcg
    54541 atgacgatct caccatattt cggcacgcct tccattttgc ggcgggcttc actgcctgcc
    54601 tccagagtgg caatccatgc cagatattca tcccacgggc aggtgagcgc cgcttccagg
    54661 caatcgataa ccccaaggtg gtgaccaatc gccaggctgt aatagaccac ctgctgcgcc
    54721 tcggcgggcg tggcatcgtt ctcatcaata aatttacggc tcagttgact gaacaccacc
    54781 ttttcactca tcggatacgc gcctcttcaa caacatgatt cagatggctg acaatctcgt
    54841 tcagacgcgg atcgttttcc gcttccagcc agcgtgcaac ctgttcttcg ccctgcccta
    54901 actgtgtaag gtaatcatcg gcaatctgac gaccgtaacg ataacccgcc aggcgacgtg
    54961 cttcgcgatc cactttcacg cgcagcggct gcaccatatc accatgcagg atctccgccg
    55021 gttgttcatc cagttcaccc ggcccacggg cgtgaatttt ctgctccagc aggccgagcg
    55081 ccattgcaaa gccgtacagc gtggcggcag gcgttggcgg gcagccaggg atataaacat
    55141 ccacagggac aattttatcc gtaccgcccc acacgcagta gagatcgtgg aagatcccgc
    55201 cactgttacc gcaggcaccg taggagatac aaattttcgg gtccggcgcg gactgccacg
    55261 cacgcagcgc aggggatcgc attgcacggg tgaccgcgcc ggtaaacagt aaaatatccg
    55321 catgacgcgg tgaaggaacg actttaatgc cgaagcgttc tgcatcaaac agcggcgaaa
    55381 gcgtggcgaa aatttcgatt tcgcaaccgt tgcagccgcc gcagtccacg cggtaaacat
    55441 aggcagaacg tttgattttt ttcagtaacg acgccttcat gctggcgatg gattcatcca
    55501 ccgtcatggg gaccggaatg ccgttggcgt cacgggggcc taataaattg ctcatcagat
    55561 ggcctctttc atatggcgag tcagttcaat acggtcggac ggcaccaggc atttctggcg
    55621 cttacattcc gggcaagtct caaagctttc gcggtggttt tccgcgcggc tgtcgccgtt
    55681 gtgcttaagc agcgcaatgg cgtagtcgat ctctttctgg acggcgaaag gacgattgca
    55741 gacgcggcag ttgcacagcg cgaagcggga ctgttgcagg aagtcttctt tcttccacac
    55801 cgccagttcg tactcttgcg acagtttgat cgccgccgtc gggcagactt cttcgcagcg
    55861 tccacagaag atgcagtgcc caagattaaa ctcccaggca agctctcctg tggcgaggtc
    55921 agtttcaacc gttaaggcgt ttgacgggca ggcattgacg caggccgcgc agccgatgca
    55981 ctgctgcggg ttctgctctg gcttaccacg gaagttttta tcaaccgcaa tcggctccag
    56041 cggataagac gaggtcgccg tgccggtttt gatgactttt ttgataaagg taaacatggc
    56101 gattccttat ttcagcggcg agtttttacg ctcaatgctg taacgctcga gttctttgta
    56161 cggcaccact ttgctcttct tcttacgcac atcgaccacg gtcatgcggt cggtacagga
    56221 gtagcaaggg tcgaggctac cgataatcag cggcgcatcg gaaacggtgt tgccgcgcag
    56281 catgtagcgc agggtcggcc agttcgcgta ggtcgcggca cggcagcgcc agcggtacag
    56341 cttctggttg tcgccggtca tgctccagtg gatatcatcg ccgcgcggcg cttcggcaaa
    56401 gcccagcgca aagcggtgcg gaatgtaggt aaagccttcc accatcagtg ggccacccgg
    56461 caggttatcc agaccgtagt cgatcatgtt cagcgcggta tagacttcgt tgatacgcac
    56521 tttcagacgg gaaataacgt cgcagccctg ctcgctgtgg acttccattg gcagcaggcc
    56581 atagccgaca aacgggtgat cggcgcgggt atcacgggcg tgaccgctgg cacggaccat
    56641 cgggccgacg ttactgaagt cgcgagcgat ttccgggtcc agacgaccaa tgccgacagt
    56701 gcgctgttcc atgttcggag tgctcagcag cacatccacc agctcctgca cttcacgacg
    56761 catctgttgt gccagctggc gggtctggat catgtcgtct ttcagcagat cgcgacgaat
    56821 cccgccgatc aagttcaggc cgtaggtttt acgcgcaccg gtaaggatct ctgccatttt
    56881 catggaggtt tcacgcacgc ggaagaactg cataaagccg gagtcgaagc cggtaaagtg
    56941 acaggccagg ccaaggttga gcagatgcga gtgcaagcgt tctacctcca gcagaatggc
    57001 gcggatcatc tgcgcacgtt ctggcacctg aatacccatc gcgttttcca ccgacgtggt
    57061 gtaggcggtg ctgtgggcaa agccgcagat cccgcacaca cggtcagaga ggaaggtcac
    57121 ttcgttataa cccatacggg tttccgccag tttttccatg ccgcgatgga cgtagaacag
    57181 acggtagtcg gcgtcgataa tgttttcgcc atcgacgaac agacggaagt ggcccggttc
    57241 atcagaagtg acgtgcagcg gaccaatcgg cacgacgttg tttttcttgt cgcccagttc
    57301 gttgatgaac tcgtaggttt cagcatcggt ggtcggtgcc ggacgctgac gataatccat
    57361 gctgtcttta cgcagcggat aaagttcatc cggccagtca tccggcagca ccagacgacg
    57421 ttcatccggc agaccaaccg gaatcaaacc gtacatatcg cgcacttcac gctcgcccca
    57481 caccgccgcc ggaacgcgcg gcgtcacgga cggatattcc ggtttgttgg cgtcaacttc
    57541 gacgcgaacc gtaatccaac acttagtgcc cttctccatc gacagcacgt agtaaacggc
    57601 gtaatgacca ttcagtttgc gttcgtcgtt accaaacagc accgacagcc agccaccctg
    57661 tttgtagtaa agaaactcca ccacttccgg caggtagttc acctttacgg tgacagtcag
    57721 ctgatctttg gtctgccagg cgtggtccag cacgacgccc ggaaatgcct cattcagcgc
    57781 ggcgagataa tgttgaccta atttttcttc agacatgctc aaactctctt taatcacgcc
    57841 gccagcaagg agacgaacgc taaaaatgca aagccaaacc cagcccaggt aatgcgcgga
    57901 gtaatatcaa gacgcagacg ggccatgctg ttttcgaaca gcgcgataac caggacgccg
    57961 accaccagtt ttacgatggc aatcaccagc gccagcagca gtccaccggc ggtgaaggtt
    58021 tccatttgtc cccacggaat aaacaccccg acgaacatct gcaacaccac cagctgtttc
    58081 aggctgatac cccatttcat gacgccaaag ccgctgccgc tgtattcaga gagcgggcct
    58141 tcctgcagct cctgctcggc ttccgccagg tcgaacggca gtttgcccat ttcgataaag
    58201 gtggcgaacg cacaggcaca aagcgccagt accagcggga tgctctggct cagcggccag
    58261 tgataaacgg tgtcggtgat gttgctgatg ttggtggaac cggcaacctg tgcggcaacc
    58321 cacagaccaa gcagcagcat cggttcgacc agcacgccaa gcatcgcttc acggctcgcg
    58381 ccgatagcgg taaacgggct accggtatcc agaccagaaa tggcaaagaa gaaacgcgcg
    58441 atggcaaaga gatacagtaa ggtgatcaaa tcacccagtt gcggcagcgg agaaccgacg
    58501 gtcaccaccg gcagcgcagt agcgatagtc agcatgacgc ccaccatcac atacggcgtc
    58561 aggcggaaca cccagccgga ggcatccggg ccgacgctct gacgccccag cagtttgata
    58621 atgtcgcgat actcctgcaa cacgcccggc ccgcgacggt tatgcaagcg ggcgcgcgcc
    58681 acgcgggtta taccggagag cagcggcgca acggcaaata acaccagcgc ctgaattaac
    58741 ggatataaaa cactcattct caggctcctc gtgaaacaat aatcaccacc agtaccgcca
    58801 gttcaaccag cgccatccgg cggaacagca acgcactccc ctcgcactgc cagcccggca
    58861 ccagagacac cggattcagc catttgcgta gtttcagcac cggcgcaaac gcctgtttca
    58921 ccggcatggc aaaaccgtga gcggtaatca ccattgattt ttcgtggtcg taaccgcaca
    58981 cccaggccgc accgcgggaa cgcgatggca aacgatcgcc tttgcaaatc gccataatga
    59041 tgaatggcag cagcgggcag gcaatcagca gcaacgtgat catcggttga gaaacggtgg
    59101 tgttagcagg ctccagcggc agaggtacag cagcagagag catcggcagt agccacggcg
    59161 cagcaacacc gccaattacg cagcaaatcg ccagtgccac tacgcttacg ctcatcagga
    59221 gcggcgcaca ggtggcgttt tcggcttctt tggtgcgcgg cgcgccgagg aacgtgacgc
    59281 catagacttt cgccatacac atcaccgcca gcgcaccggt aattgccagc cccacagcga
    59341 gcagcggccc cagcagacgg gcaacaaacg cgccactatt gctcagtttg aaaaatgatt
    59401 gatagataac ccattccccg gcaaaaccat tcagcggcgg cagcgcagcc attgccatca
    59461 gcccgactaa catggcgatg gagataaccg gcattttctt gccaatacca ccgagttttt
    59521 cgatatcgcg atgaccggta cggaaccaga cgctccccgc cccgaggaac agtacgcttt
    59581 tgaacaggct atggttaagc agatggtaca gaccaccgac caggccaaga gcaatcagcg
    59641 ccggttgttc gagcgcgata cccgttacgc cagcgcccag ccccagcagg atgatgccga
    59701 tattttccag ggtgtggtaa gccagcaggc gctggatgtt gtgctccacc agcgcataca
    59761 gaccaccgac aaacgcggtg atcatgccga gcaccagcag cgcgatcccc caccacagcg
    59821 gtgcattacc gcccagcagt gacagggtta aaatgcccag caggccaatt ttcatgacta
    59881 ccgtagaaaa caacgcggca gctggcgcag aggcgttcgc atgtgcctgc ggcacccagc
    59941 cgtgcagcgg aataatcccg gccagcaggc caaagccaat cactccgagc agccagatat
    60001 cggaaccgag cggcagctgt tgcatacgca tatccagcag gcgcagatcc agcgtgccgt
    60061 aacgctgcca cagcagccag caagcaatcg ccagcagcag agtgccaaga cgccccagcg
    60121 caaaccacag tttgccctct ttgctgttgc tggtgaggaa caccgcgcac agggccatga
    60181 tttcggccat taccacgaac atgccgaggt tgctggcaat gacggcgcag acggcggcag
    60241 ccatcaacat attgatctgc aagccgttgc acttcacctg cgcgtggcga tgccagtcaa
    60301 tgttgtagag gctgacaaac agaccgcaca gaccgagcgt aatcagccaa atcgcgttaa
    60361 gcggagagat ttgcacatcg tagcttacca gcgacagcgc accgctcacg ccaaccgcgc
    60421 cagtcagtac agtgaagccc gcggctgccg tatacagact accaaccgcg ccgccaattc
    60481 cagctatcca gccacttaac gctttttgaa aagaaaagag aaatgccaga acagcggcgg
    60541 cgacaaacca cgccacgccg ctattgatca gggaaattgc gctcatttag cctctccact
    60601 ttgagcctgc tgaaacaagg tgagatcgcc aaagtccgtg ttaaaggtca gctcacgctt
    60661 acgtttgctg acgcgggcga tatcggtgtt atccaccaga tgcagggctt tagtcgggca
    60721 catccgcacg caggccggac cttgttcatc aaagctacaa aggtcacatt tgacggcgat
    60781 cgcgcgaata cctggcaccc agtcaagcaa tgtgctgaca cgcgccggag caggcggtgc
    60841 cggtggcgct ttcggggtat tggcgtttgc cggaatatcc agcggacggc tgccggaaaa
    60901 ttcaattgcg ccaaacgggc aggcgatgcc gcacagcttg cagcttacgc acaggctttc
    60961 attcaactgc acggccccat cgacgcgggt gatggcgtta accgggcaga ccaccgcgca
    61021 gggtgcatct tcacagtggt gacagagctg cggcgcagat tctttttcat tcagcatcac
    61081 tctcaggcgc ggcattgatt gcaggccgtg ctggcgatgc gtctctgaac aggcggcctc
    61141 acaagtgtgg cagccgatac agagcgtgga gtcagcaatt acaaaacgat tcaccaggca
    61201 ttcctcaggt gattgtcatt tttgacgaaa acatgccgat gaaatgtcat tttcgacact
    61261 catcgacacg cccatcccca aacaggcgta acgcctgcaa aacgggcaaa gcctcagctc
    61321 atgctgccgg gctttgtccc tttaccagtt ggcttaaatt caccggcaca ttgttttcaa
    61381 tagcggtgta taagctgtcg taaacgccag cgattttttc gagatagtgt tccagaacct
    61441 gttcgcgatc gcggatgttg accttgccat caatcatgcc gtcaatgctc agttgcgcct
    61501 gcgcaatccg ctgtttgtct tcgccatctt ttgtttcgac gtaatactcg atggtgtggc
    61561 tgttaaagcc atccgccagg gtgacgtaaa tgcgaaaatg ttcaaaaagg acgaaacaga
    61621 gttctttgtc cggcgcgcag ctcatggcgt gatgcaggcg cgctttcgat aacgtgatcc
    61681 cctgaaccag cgaattgcag tagatgtgcc actggtcctg taggcgacga tgccgctgtg
    61741 cgatgtaatc ggctttctcg cttatttccc aaatagtcat tgtcaggtta cccgtttaac
    61801 agagatgcca gctttaagca ttttttgtgc caacttttaa tttattgtta ttaaagagat
    61861 ttttaagcta aagatgaatt tcgtcgccgt gtcgacgtgt catttcgaca tcatcgacat
    61921 tattcaccgc agggataatc aacactggca caattattgc ttgtagctgg caatagttaa
    61981 tgggaggcga tatgcacgaa ataaccctct gccaacgggc actggaattg atcgaacagc
    62041 aggccgcaaa acacggcgca aaacgcgtaa ctggggtctg gctcaaaatt ggcgcatttt
    62101 cttgtgtcga aaccagctct cttgcctttt gttttgatct ggtttgccgc ggcagcgtgg
    62161 cggaaggttg taaactgcac ctcgaagaac aagaggccga atgctggtgt gaaacatgcc
    62221 aacagtatgt gacgctactg acccagcgcg tccgccgctg tccacagtgt catggtgaca
    62281 tgctgcagat tgtggcagac gacggtttac agattcggcg gatagaaata gaccaggagt
    62341 gagcgatgtg tacaacatgc ggttgcggtg aaggcaacct gtatatcgag ggtgatgaac
    62401 ataaccctca ttccgcgttt cgtagcgcgc catttgcccc ggcggcacgc ccgaagatga
    62461 aaatcaccgg cattaaagcg cctgaattta cccccagcca gactgaagaa ggcgacctgc
    62521 attacggtca tggcgaagcg ggcactcacg caccgggcat gagccagcgt cggatgctgg
    62581 aagtcgaaat tgacgtgctg gacaaaaata accgtctggc tgaacgcaac cgcgcgcgct
    62641 ttgctgcccg caagcaactg gtgctcaacc tggtttccag ccctggttcc ggtaaaacca
    62701 ccctgctgac ggaaacccta atgcgcctga aagacagcgt tccgtgcgca gttattgaag
    62761 gcgaccagca aaccgtgaac gatgccgcac gcattcgcgc taccggcaca ccagcgattc
    62821 aggtgaacac cggtaaaggc tgccatcttg acgcacagat gattgccgac gccgcaccgc
    62881 gtctgccact ggacgataac ggtattctgt ttatcgaaaa cgttggcaac ctcgtatgcc
    62941 cggccagctt cgatctcggt gaaaaacaca aagtggcggt gctttccgtt accgaaggtg
    63001 aagacaaacc gctgaaatat ccgcatatgt ttgccgccgc ctcgctgatg ctgctcaaca
    63061 aagttgacct gttgccgtat ctcaactttg acgttgaaaa gtgcatcgcc tgcgcccgcg
    63121 aagtcaatcc agaaattgaa atcatcctta tttccgccac cagcggcgaa gggatggacc
    63181 agtggctgaa ctggctggag acacagcgat gtgcataggc gttcccggcc agatccgcac
    63241 cattgacggc aaccaggcga aagtcgacgt ctgcggcatt cagcgcgatg tcgatttaac
    63301 gttagtcggc agctgcgatg aaaacggtca gccgcgcgtg ggccagtggg tactggtaca
    63361 cgttggcttt gccatgagcg taattaatga agccgaagca cgcgacactc tcgacgcctt
    63421 acaaaacatg tttgacgttg agccggatgt cggcgcgctg ttgtatggcg aggaaaaata
    63481 atgcgttttg ttgatgaata tcgcgcgccg gaacaggtga tgcagttaat tgagcatctg
    63541 cgcgaacgtg cttcacatct ctcttacacc gccgaacgcc ctctgcggat tatggaagtg
    63601 tgtggcggtc atacccacgc tatctttaaa ttcggcctcg accagttact gccggaaaac
    63661 gttgagttta tccacggtcc ggggtgcccg gtgtgcgtac tgccgatggg tagaatcgac
    63721 acctgcgtgg agattgccag ccatccggaa gtcatcttct gtacctttgg cgacgccatg
    63781 cgcgtgccgg ggaaacaggg atcgctgttg caggcaaaag cacgcggtgc cgatgtgcgc
    63841 atcgtttact cgccgatgga tgcgttgaaa ctggcgcagg agaatccaac ccgcaaagtg
    63901 gtgttcttcg gcttaggttt tgaaaccact atgccgacca ccgctatcac tctgcaacag
    63961 gcgaaagcgc gtgatgtgca gaatttttac ttcttctgcc agcacattac gcttatcccg
    64021 acgttgcgca gtttgctgga acagccggat aacggtatcg atgcgttcct cgcgccgggt
    64081 cacgtcagta tggttatcgg caccgacgcc tataatttta tcgccagcga ttttcatcgt
    64141 ccgctggtgg ttgctggatt cgaacccctt gatctactac aaggcgtggt catgctggtg
    64201 cagcagaaaa tagcggccca cagcaaggta gagaatcagt atcgtcgagt ggtaccggat
    64261 gccggtaacc tgctggcgca acaggcgatt gccgatgtgt tctgtgtcaa cggcgacagc
    64321 gaatggcgcg gcttaggcgt gattgaatct tctggcgtgc acctgacgcc ggattatcaa
    64381 cgattcgatg ccgaagcaca tttccgcccg gcaccgcagc aggtctgcga tgacccgcgc
    64441 gcgcgttgtg gtgaggtatt aacgggcaaa tgtaagccgc atcaatgccc gctgtttggt
    64501 aacacctgta atcctcaaac cgcgtttggt gcgctgatgg tttcctccga aggagcgtgc
    64561 gccgcgtggt atcagtatcg tcagcaggag agtgaagcgt gaataatatc caactcgccc
    64621 acggtagcgg cggccaggcg atgcagcaat taatcaacag cctgtttatg gaagcctttg
    64681 ccaacccgtg gctggcagag caggaagatc aggcacgtct tgatctggcg cagctggtag
    64741 cggaaggcga ccgtctggcg ttctccaccg acagttacgt tattgacccg ctgttcttcc
    64801 ctggcggtaa tatcggcaag ctggcgattt gcggcacagc caatgacgtt gcggtcagtg
    64861 gcgctattcc gcgctatctc tcctgtggct ttatcctcga agaaggattg ccgatggaga
    64921 cactgaaagc cgtagtgacc agcatggcag aaaccgcccg cgcggcaggc attgccatcg
    64981 ttactggcga tactaaagtg gtgcagcgcg gcgcggtaga taaactgttt atcaacaccg
    65041 ctggcatggg cgcaattccg gcgaatattc actggggcgc acagacgcta accgcaggcg
    65101 atgtattgct ggtgagcggt acactcggcg accacggggc gactatcctt aacctgcgtg
    65161 agcagctggg gctggatggc gaactggtca gcgactgcgc ggtgctgacg ccgcttattc
    65221 agacgctgcg tgacattccc ggcgtgaaag cgctgcgtga tgccacccgt ggtggtgtaa
    65281 acgcggtggt tcatgagttc gcggcagcct gcggttgtgg tattgaactt tcagaagcgg
    65341 cactgcctgt taaacctgcc gtgcgtggcg tttgcgaatt gctgggactg gacgccctga
    65401 actttgccaa cgaaggcaaa ctagtaatag ctgttgaacg caacgcggca gagcaagtgc
    65461 tggcagcgtt acattcccat ccactgggga aagacgcggc gctgattggt gaagtggtgg
    65521 aacgtaaagg tgttcgtctt gccggtctgt atggcgtgaa acgaaccctc gatttaccac
    65581 acgccgaacc gcttccgcgt atatgctaat aaaattctaa atctcctata gttagtcaat
    65641 gaccttttgc accgctttgc ggtgctttcc tggaagaaca aaatgtcata tacaccgatg
    65701 agtgatctcg gacaacaagg gttgttcgac atcactcgga cactattgca gcagcccgat
    65761 ctggcctcgc tgtgtgaggc tctttcgcaa ctggtaaagc gttctgcgct cgccgacaac
    65821 gcggctattg tgttgtggca agcgcagact caacgtgcgt cttattacgc gtcgcgtgaa
    65881 aaagacaccc ccattaaata tgaagacgaa actgttctgg cacacggtcc ggtacgcagc
    65941 attttgtcgc gccctgatac gctgcattgc agttacgaag aattttgtga aacctggccg
    66001 cagctggacg caggtgggct atacccaaaa tttggtcact attgcctgat gccactggcg
    66061 gcggaagggc atatttttgg tggctgtgaa tttattcgtt atgacgatcg cccctggagc
    66121 gaaaaagagt tcaatcgtct gcaaacattt acgcagatcg tttctgtcgt caccgaacaa
    66181 atccagagcc gcgtcgttaa caatgtcgac tatgagttgt tatgccggga acgcgataac
    66241 ttccgcatcc tggtcgccat caccaacgcg gtgctttccc gcctggatat ggacgaactg
    66301 gtcagcgaag tcgccaaaga aatccattac tatttcgaca ttgacgatat cagtatcgtc
    66361 ttacgcagcc accgtaaaaa caaactcaac atctactcca ctcactatct tgataaacag
    66421 catcccgccc acgaacagag cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc
    66481 aaaagtaaag agatgctgct gatcaatctc cacgagcggg acgatttagc cccctatgaa
    66541 cgcatgttgt tcgacacctg gggcaaccag attcaaacct tgtgcctgtt accgctgatg
    66601 tctggcgaca ccatgctggg cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc
    66661 actaccaatc tgaatttact gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac
    66721 gccctcgcct atcaggaaat ccatcgtctg aaagaacggc tggttgatga aaaccttgcc
    66781 ctgaccgagc agctcaacaa tgttgatagt gaatttggcg agattattgg ccgcagcgaa
    66841 gccatgtaca gcgtgcttaa acaagttgaa atggtggcgc aaagtgacag taccgtgctg
    66901 atcctcggtg aaactggcac gggtaaagag ctgattgccc gtgcgatcca taatctcagt
    66961 gggcgtaata atcgccgcat ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg
    67021 gaaagcgatc tgtttggtca tgagcgtggg gcttttaccg gtgccagcgc ccagcgtatc
    67081 ggtcgttttg aactggcgga taaaagctcc ctgttcctcg acgaagtggg cgatatgcca
    67141 ctggagttac agccgaagtt gctgcgtgta ttgcaggaac aggagtttga acgtctcggc
    67201 agcaacaaaa tcattcagac ggacgtgcgt ctaatcgccg cgactaaccg cgatctgaaa
    67261 aaaatggtcg ccgaccgtga gttccgtagc gatctctatt accgcctgaa cgtattcccg
    67321 attcacctgc cgccactacg cgagcgtccg gaagatattc cgctgctggc gaaagccttt
    67381 accttcaaaa ttgcccgtcg tctggggcgc aatatcgaca gcattcctgc cgagacgctg
    67441 cgcaccttga gcaacatgga gtggccgggt aacgtacgcg aactggaaaa cgtcattgag
    67501 cgcgcggtat tgctaacacg cggtaacgtg ctgcagctgt cattgccaga tattgtttta
    67561 ccggaacctg aaacgccgcc tgccgcaacg gttgtcgccc tggagggcga agatgaatat
    67621 cagttgattg tgcgcgtgct gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct
    67681 gcgcaacgtc tggggctgaa acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt
    67741 gataaatcgg cattgattta actgcaaatt gccggacaga tctgcctgtc cggcatacta
    67801 ttcatgaggt tttttcggac gatatttttc cggcagttct ggcaccggac gcttgtcatc
    67861 gatgagatga cgcacggtta agatcggatg acgccacagc attctcggcc cggcccaacg
    67921 cataatctgt ttcatctctt cacgctttgc aggctggtaa cagtgcaccg gacactgctt
    67981 acaggctggt ttctcttcgc cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa
    68041 caacgcctcg taatgctccg gctccgctga cgcctgcggg catttcgttt gataaagatc
    68101 gatcattttt ttaatcgtca gtttttcacg agagatacgc ttgccggaca tgctgcctcc
    68161 acctcattaa gatgcattta tattacatct taatcttaaa gggcactatg actccaaaga
    68221 agaagggtta gccaaccgat acaattttgc gtacttgctt cataagcatc acgcaaaagc
    68281 tgcaaaacag catctttccc ggaaccagca tcaagaactc gccgttcgct tcttcccctg
    68341 aaatgattaa ctccggtatc atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc
    68401 acaccccatt taatatcagg gaaccggaca taaccccatg agtgcaatag aaaatttcga
    68461 cgcccatacg cccatgatgc agcagtatct caggctgaaa gcccagcatc ccgagatcct
    68521 gctgttttac cggatgggtg atttttatga actgttttat gacgacgcaa aacgcgcgtc
    68581 gcaactgctg gatatttcac tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat
    68641 ggcggggatt ccctaccatg cggtggaaaa ctatctcgcc aaactggtga atcagggaga
    68701 gtccgttgcc atctgcgaac aaattggcga tccggcgacc agcaaaggtc cggttgagcg
    68761 caaagttgtg cgtatcgtta cgccaggcac catcagcgat gaagccctgt tgcaggagcg
    68821 tcaggacaac ctgctggcgg ctatctggca ggacagcaaa ggtttcggct acgcgacgct
    68881 ggatatcagt tccgggcgtt ttcgcctgag cgaaccggct gaccgcgaaa cgatggcggc
    68941 agaactgcaa cgcactaatc ctgcggaact gctgtatgca gaagattttg ctgaaatgtc
    69001 gttaattgaa ggccgtcgcg gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac
    69061 cgcgcgccag cagttgaatc tgcaatttgg gacccgcgat ctggtcggtt ttggcgtcga
    69121 gaacgcgccg cgcggacttt gtgctgccgg ttgtctgttg cagtatgcga aagataccca
    69181 acgtacgact ctgccgcata ttcgttccat caccatggaa cgtgagcagg acagcatcat
    69241 tatggatgcc gcgacgcgtc gtaatctgga aatcacccag aacctggcgg gtggtgcgga
    69301 aaatacgctg gcttctgtgc tcgactgcac cgtcacgccg atgggcagcc gtatgctgaa
    69361 acgctggctg catatgccag tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat
    69421 tggcgcattg caggatttca ccgccgggct acagccggta ctgcgtcagg tcggcgacct
    69481 ggaacgtatt ctggcacgtc tggctttacg aactgctcgc ccacgcgatc tggcccgtat
    69541 gcgccacgct ttccagcaac tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc
    69601 accggtacag gcgctacgtg agaagatggg cgagtttgcc gagctgcgcg atctgctgga
    69661 gcgagcaatc atcgacacac cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg
    69721 ctataacgaa gagctggatg agtggcgcgc gctggctgac ggcgcgaccg attatctgga
    69781 gcgtctggaa gtccgcgagc gtgaacgtac cggcctggac acgctgaaag ttggctttaa
    69841 tgcggtgcac ggctactaca ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa
    69901 ctacatgcgt cgccagacgc tgaaaaacgc cgagcgctac atcattccag agctaaaaga
    69961 gtacgaagat aaagttctca cctcaaaagg caaagcactg gcactggaaa aacagcttta
    70021 tgaagagctg ttcgacctgc tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc
    70081 gctggcggaa ctcgacgtgc tggttaacct ggcggaacgg gcctataccc tgaactacac
    70141 ctgcccgacc ttcattgata aaccgggcat tcgcattacc gaaggtcgcc atccggtagt
    70201 tgaacaagta ctgaatgagc catttatcgc caacccgctg aatctgtcgc cgcagcgccg
    70261 catgttgatc atcaccggtc cgaacatggg cggtaaaagt acctatatgc gccagaccgc
    70321 actgattgcg ctgatggcct acatcggcag ctatgtaccg gcacaaaaag tcgagattgg
    70381 acctatcgat cgcatcttta cccgcgtagg cgcggcagat gacctggcgt ccgggcgctc
    70441 aacctttatg gtggagatga ctgaaaccgc caatatttta cataacgcca ccgaatacag
    70501 tctggtgtta atggatgaga tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc
    70561 gtgggcgtgc gcggaaaatc tggcgaataa gattaaggca ttgacgttat ttgctaccca
    70621 ctatttcgag ctgacccagt taccggagaa aatggaaggc gtcgctaacg tgcatctcga
    70681 tgcactggag cacggcgaca ccattgcctt tatgcacagc gtgcaggatg gcgcggcgag
    70741 caaaagctac ggcctggcgg ttgcagctct ggcaggcgtg ccaaaagagg ttattaagcg
    70801 cgcacggcaa aagctgcgtg agctggaaag catttcgccg aacgccgccg ctacgcaagt
    70861 ggatggtacg caaatgtctt tgctgtcagt accagaagaa acttcgcctg cggtcgaagc
    70921 tctggaaaat cttgatccgg attcactcac cccgcgtcag gcgctggagt ggatttatcg
    70981 cttgaagagc ctggtgtaat aacaattccc gatagtcttt tgctatcggg aatattaacg
    71041 acaactgacg aataaaataa aaacaccctg tataatagga aagcttattt tacagggtaa
    71101 aaccatgcca tctacacgct atcaaaaaat caatgcccat cactatcgcc atatatgggt
    71161 cgttggtgat attcatggtg aatatcagtt attacaatcc cgcttacatc aactctcttt
    71221 tttccccgaa atcgacttac ttatttctgt cggcgataat attgatcgtg gaccggagag
    71281 tcttgacgtc ctgcgcctgc taaaccaacc ctggtttacg tcggttaaag gcaaccacga
    71341 agcgatggcg cttgaggcat tcgaaactgg cgatggcaat atgtggcttg ccagcggtgg
    71401 tgactggttt ttcgatttaa atgattcaga gcaacaagag gcaatagatc tgttgctgaa
    71461 attccatcac cttccacata ttattgaaat cactaacgac aacataaaat atgccatcgc
    71521 acatgcagat tatccgggga gtgaatatct ctttggtaaa gaaatagcgg agagcgaatt
    71581 actctggcct gttgatcgtg tgcagaaatc gcttaatggc gagttacaac aaataaacgg
    71641 cgctgattat tttatatttg gacatatgat gtttgataac attcagacgt tcgctaacca
    71701 gatttatatt gataccggat cgccgaacag cgggcggctg tcattttata aaataaagta
    71761 gtctcatgct tcttctgtga agcatgagta acccggtgtt attgcaggcc attattcatt
    71821 tttcgctacc agcaaagaga gatcctgctt caccagcgcg cgactggcac tctccggcaa
    71881 accgtcgtct gtaataatct gatcaaactc gcttaatggt aacgccagcc atgtcgccac
    71941 ctgaccatat ttcgtcgcat cacagaccaa aactcgctgg cggctggcac tggcaatcgc
    72001 ccgtttcacc gtgactttat cttccgctgg cgtagaaatc ccccgcacac tccatgacga
    72061 tgcagaaata aaagcctgat caatcatcag gctgcgcagc atggtcgcag cggcttcccc
    72121 gacacaggaa cggttttccc gacacactgc accgccagtg tgaataattg tgcaattact
    72181 gttgtcgagc aagtagtccg caataacgaa atcgtttgtg accacagtca gtgactccat
    72241 gtgaatcaga tgctgtgcta tcgctaacgt ggtcgttccc gcatccagat agatacaact
    72301 tcccggctga acaagacttg ccgccagctt gccaatagcc gctttttgcg tcattgccag
    72361 cgcagttttt acctgatgag aaggttcatg cgccacgcgt cccggagact ggacgcctcc
    72421 ggacaccagc acaacggctc cctgctgctc cagtttttgt aaatcccgac gaatggtcat
    72481 atgtgacaca ttcattctgt ccgttagttc agcaatactg acaatgcctt tttcagctac
    72541 catctcaagg atgatttggc gacgctctac gggtatcaac ttttgctcct tcctttgtcc
    72601 tgctgacatt ctacgctatt tgcctgcgaa acgtgcgcgg cgcaactaac gcttagttca
    72661 cataaaataa cacacaatgt taatttatgt gaatcagatc accatatcgt tatcttccag
    72721 cgcttatatt cacaatatca aacaaaatat cacttaaatt aacaaggaga gcagatgaaa
    72781 acgggatctg agtttcatgt cggtatcgtt ggcttagggt caatgggaat gggagcagca
    72841 ctgtcatgtg tccgcgcagg tctttctacc tggggcgcag acctgaacag caatgcctgc
    72901 gctacgttga aagaggcagg tgcttgcggg gtttctgata acgccgcgac gtttgccgaa
    72961 aaactggacg cactgctggt gctggtggtc aatgcggccc aggttaaaca ggtgctgttg
    73021 ggtgaaacag gcgttgcaca acatctgaaa cccggtacgg cagtaatggt ttcttccact
    73081 atcgctagtg ctgatgcgca agaaattgct accgctctgg ctggattcga tctggaaatg
    73141 ctggatgcgc cagtttctgg tggtgcagta aaagccgcta acggtgaaat gactatcatg
    73201 gcctccggta gcgatattgc ctttgaacga ctggcacccg tgctggaagc cgttgccgga
    73261 aaagtttatc gcataggtgc agaaccggga ctaggttcga ccgtaaaaat tattcaccag
    73321 ttgttagcgg gcgtacatat tgctgccgga gccgaagcga tggcacttgc agcccgtgcg
    73381 gggatcccgc tggatgtgat gtatgacgtc gtgaccaatg ccgccggaaa ttcctggatg
    73441 ttcgaaaacc ggatgcgtca tgtggtggat ggcgattaca ccccgcattc agccgtcgat
    73501 atttttgtta aggatcttgg tctggttgcc gatacagcca aagccctgca cttcccgctg
    73561 ccattggcct caacagcatt gaatatgttc accagcgcca gtaacgcggg ttacgggaaa
    73621 gaagacgata gcgcagttat caagattttc tctggcatca ctctaccggg agcgaaatca
    73681 tgatcaagat tggcgttatc gccgatgatt ttaccggcgc gacggatatc gccagttttc
    73741 tggtggaaaa cggtctacca acggtacaaa ttaacggtgt tccaacaggt aaaatgccgg
    73801 aagcaatcga cgcactggtg atcagcctga aaacgcgctc ctgtccagtg gttgaagcca
    73861 cacagcaatc gctggcggct ctgagctggt tgcaacagca aggttgcaaa cagatctatt
    73921 tcaaatactg ctctactttc gacagtacgg cgaaaggtaa tattggcccg gttaccgatg
    73981 ccttaatgga tgctctcgac acgccgttta cggtcttctc tccggccctg ccggtcaacg
    74041 gacgtacggt ttatcagggg tatttgttcg taatgaatca actgctggcc gaatccggga
    74101 tgcgccatca cccggtaaat cccatgaccg acagctatct tccccgtctg gttgaagcgc
    74161 aatccacagg gcgctgcggc gtcgtttcgg cacatgtttt cgaacaaggt gtggatgccg
    74221 ttcgtcaaga gctggctcgc ttacagcaag agggctaccg ctacgcggtg cttgatgcgc
    74281 tgaccgaaca ccatctggaa attcagggag aagccttgcg cgatgcccca ctggtaacgg
    74341 gcggttctgg tctggcgatt ggcctggccc ggcagtgggc gcaagaaaac ggtaaccagg
    74401 ctcgcaaagc agggcgtccg ctcgctgggc gcggcgtagt gctctccggt tcatgctctc
    74461 aaatgaccaa ccgccaggta gcacattacc gtcaaattgc accagcccgt gaagttgatg
    74521 tggcacgctg cctctcaatt gaaactctgg ccgcttatgc acacgaactg gcagagtggg
    74581 ttctgggcca ggaaagtgta cttgctccac tggtttttgc caccgccagc actgacgcat
    74641 tggcagcaat tcaacagcaa tacggtgcac aaaaagccag tcaggcagta gaaacactgt
    74701 tttctcaact agcggcgcgg ttagcagcgg aaggcgtgac acgctttatt gtcgcaggcg
    74761 gtgagacctc cggcgtagtc acacagagcc tgggaataaa agggtttcat attggcccaa
    74821 ccatttcccc cggcgtgccg tgggtaaacg cactggataa gcctgtctca ctcgccctta
    74881 aatctggcaa cttcggtgat gacgcctttt tttcacgagc ccaaagagag tttttatcat
    74941 gagcgatttc gcaaaagtag agcagtcttt gcgagaggag atgacgcgga ttgccagttc
    75001 attctttcag cgcggctatg caaccggttc ggctggcaat ctgtcgctgc ttttacctga
    75061 cgggaattta ctggcgacac cgacaggttc atgcctgggc aatctcgatc cgcagcggct
    75121 ttccaaagtc gccgcggatg gcgaatggtt aagtggtgac aaaccctcga aagaggtgct
    75181 ctttcatctg gcgctgtatc gcaacaatcc gcgttgtaaa gcggtggtgc atttgcacag
    75241 cacatggtcg acggcgcttt cctgcctgca agggctggac agcagcaacg ttattcgtcc
    75301 gttcacacca tacgtggtga tgcggatggg aaatgtcccg ctggtgcctt attaccgacc
    75361 gggcgataaa cgcatcgcac aggatctggc ggaactggca gcagacaatc aggctttttt
    75421 actggcaaat catggcccag tggtttgcgg tgaaagcctg caagaagccg ccaacaatat
    75481 ggaagagctg gaggaaacgg caaagctgat ttttattctc ggtgaccgcc cgatccgtta
    75541 tctgaccgca ggtgaaattg cggaattaag gagttaatgc aatgcctcgt tttgcagcta
    75601 atttatccat gatgttcacc gaagtgcctt ttattgaacg cttcgccgca gcgcgaaaag
    75661 ccgggttcga tgctgtggaa tttctgtttc cctatgacta ctccaccctg caaatccaaa
    75721 agcaactgga gcaaaaccat ctgacactgg cgctgttcaa taccgctccc ggagatatta
    75781 atgcggggga atggggatta tccgcccttc ccggacgtga gcacgaagca cacgcagaca
    75841 tcgacctggc actggaatat gcattggcgc tcaattgtga acaagtccat gtgatggcag
    75901 gcgtcgtgcc cgctggcgaa gatgctgaac ggtaccgggc agtatttatc gataatctcc
    75961 gctacgctgc cgaccgcttt gcgccacacg gcaagcgaat attagttgaa gcactcagcc
    76021 ccggcgttaa gcctcattat ctcttctcca gccagtatca ggcactggct attgttgaag
    76081 aagttgcgcg agataacgtg tttattcaac tagatacttt tcatgcgcaa aaagtagatg
    76141 gcaacctgac gcatttaatc cgcgactacg ccgggaaata tgcgcacgta caaattgccg
    76201 gactacctga tcgacatgaa ccggacgatg gagaaatcaa ctatccgtgg ctgttccgcc
    76261 tgttcgatga ggtgggatat cagggctgga tcggttgtga atataaacct cgtggcctca
    76321 ccgaagaagg gcttggctgg tttgacgcct ggcgctaatt cgttaaatcc ctaattacaa
    76381 cgtacccata catcccccca tctacggatg gggtaggtta actataactt tcagacaggg
    76441 tttcccatgt ccacaattac attgttatgc attgcgttaa ctggcgtaat catgctgttg
    76501 ctgctggtca tcaaggcaaa ggtacaacca ttcgttgctc tgctcctcgt cagcctgtta
    76561 gtcgcacttg cggcgggtat accggcgggc gaagtgggta aagtgatgat cgccgggatg
    76621 ggcggtgtgc ttggttccgt cactattatt attggtctgg gcgctatgct ggggcgtatg
    76681 atcgaacact ctggtggtgc agagtcactg gctaattatt tcagtcgcaa gttaggtgac
    76741 aaacgaacta tcgctgcgct gactctggca gcgttcttcc tcggtattcc cgtcttcttt
    76801 gatgtcggct ttattattct tgcgccaatc atttacggtt ttgccaaggt tgccaaaata
    76861 tcgccactca aatttggcct gcctgtcgct gggatcatgc tcactgttca cgtggcggta
    76921 ccgccgcatc caggccctgt cgccgcagcg gggttactcc acgcagacat cggctggcta
    76981 accatcatcg gtattgcgat ttctattccc gtaggggttg ttggctactt tgcagcgaaa
    77041 ataatcaata agcgccaata tgcgatgtca gtagaagtac tggaacagat gcaactggct
    77101 ccggccagtg aggaaggcgc gacaaaatta agcgataaaa taaatccacc gggcgtcgcg
    77161 ctggtcacct cgctaattgt tattcctatc gcgattatca tggcgggtac ggtttccgca
    77221 acactgatgc cgccttcgca tcccctgctt ggtacgctac agctgatcgg ctcaccaatg
    77281 gtagctctaa tgattgcgct ggtgctggca ttctggttat tggctttacg tcgcggctgg
    77341 agcttacaac acaccagcga cattatgggc tcagcgcttc ctactgcggc ggtagtgatt
    77401 ttggttactg gtgctggagg ggtatttggc aaagtgctgg tggaatcggg cgtcggcaaa
    77461 gcccttgcca acatgctaca aatgattgac ctgcctctgt taccagccgc atttattatt
    77521 tcactggcgc tgcgtgcatc gcaggggtca gcaaccgtag caatactgac aaccggcggg
    77581 ttactctcag aagcggtgat gggattgaat ccgattcagt gcgtattggt gacgctggca
    77641 gcctgctttg gtgggcttgg tgcgtcacat attaatgact cagggttctg gattgtgacc
    77701 aaatatctgg ggttatcggt agcagacggt ctgaaaacct ggacagtgtt aacgaccatt
    77761 ctcggtttta ccggattctt aattacctgg tgcgtatggg cggtaatttg accttaataa
    77821 aaaggtccga tgggcatcgg accttttatt gtgcacagaa aaggccagcc tcgcttgaga
    77881 ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat tcagcccctg
    77941 cgtttgcagg atttcacgca aacggcgcag gccttcaacc tgaatctggc gaacacgttc
    78001 acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt accccagcaa
    78061 accgaatcga cgtgccagca cttcacgctg tttggcgttc agctcgaaca gccatttgac
    78121 gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct ctttttcatc
    78181 ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt ctaccgaggt
    78241 aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat ccagttgctc
    78301 tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac gtgcggttcg
    78361 caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt
    78421 cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa
    78481 accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaggttgc cctcttcgat
    78541 aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta ccaccagacg
    78601 caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg cgcgacgcgc
    78661 aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac caaggtaaag
    78721 ctgagtcgcg tccaacacac gctgtgtggc tccctgcgat aacagttcct cttcggccaa
    78781 atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct caactccgtt
    78841 ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct gactcataag
    78901 gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcctggt tccgccgatt
    78961 tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa
    79021 tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg ccccgccttc
    79081 acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact caggtaatca
    79141 tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc agcataaaca
    79201 acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat atcaatcccc
    79261 ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt cggccagcgc
    79321 caggtggaga taggcgtact ggttgatgta ctgctgacag ttggctcggt tgtgcttgct
    79381 gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagctg gcttgttgtt cggcaacatt
    79441 ttgttagcac tctgttcacc cgaagattca gaatacgtaa ttgtcggttg cgacgcaaca
    79501 gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc gtcggcctgg
    79561 gtaatggcat ttccgccagt gattggcgta ccggaagcat tacccacctg caaggtctga
    79621 ccaacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg
    79681 ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt ataggtactg
    79741 ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat gcgtccgttt
    79801 tccatctgta ccggctgctg agctactggc tgcactggct ggatttgcgg ttgttgagta
    79861 gcctgaattt gtggctgctg taccggctga atttgcggtt gctgcgctgt agacgtcgtc
    79921 cccattttcg gcggcggcgt aatcaacata ccagaattag tatttgcagg cgcattgcca
    79981 ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc cagccatagc
    80041 gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc cgcgctcatt
    80101 tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa ggcaccctac
    80161 tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg caaaataacc aggaaaaatc
    80221 caggtatttc ctcacgtttt aagccagctc acccttcact aaagggacaa agcgcacggc
    80281 ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca aatactggtg
    80341 ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca ttagcgcagt
    80401 tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcac gtgcctgcca
    80461 accttgccat ccatcgccat gacgggttga aacattatgt aaatcaagat ttttcaggcg
    80521 gcgacgtgcc tgccactgca agcctttaat ccgttcaacc gagcaaacat gctggacaag
    80581 atgcgccagg attgccgttt gatatcccga accggtgcca atttccagca cccgcgactg
    80641 cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tatggctgcg aaattgtctg
    80701 cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg cttcatcaac
    80761 gaatttttca cgcggcacgg cggcaagtgc attcagcacc tgctcatcct gaataccttg
    80821 cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt gccaactccc
    80881 acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa atccacatgc
    80941 agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc cggaccggca
    81001 tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg atcttgctgc
    81061 gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat acctttgatt
    81121 tgatccaagg gtaaatccgg aacgttaata ttaagaatac gcccggtgcg cagcggctct
    81181 ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta atgtttatgc
    81241 ccgtcaagcg agacggcaag cgccggaaaa cctaaatgac ggccttccat cgcggcggct
    81301 accgtaccgg aataaataac atcatccccc agattcggcc cggcgttaat tccggacaca
    81361 acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacgca atcggtcggg
    81421 gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag ggaggattcc
    81481 agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac ctgaacgtca
    81541 gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac cccgtcatca
    81601 ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga caacgctggt
    81661 tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat cccaccaatt
    81721 ccagcttaat tgttgcggat acagcagcat cgctctgcgc gcggcttcaa ctttttcgcg
    81781 caccagtaaa gcttgtaatt cagtttctgc ggcgacagct gcttgttcga atgccagcgc
    81841 ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca tcagctcttt
    81901 atcgttaacg cgacgctgta attccgccag ttcttcggtg gttgcgacaa accagctacc
    81961 acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg cttttttgag
    82021 gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc aaaaactccg
    82081 tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgctgcgccc cctgcaagtt
    82141 gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt ttacgcaaat
    82201 atcgttcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa ccagggtaaa
    82261 ggcgtttcct ttcagcgccc ctaaacgcag cttgcgcttg tgccgcgcat actccagcac
    82321 ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcccg gcacgcgagc
    82381 gcataaccac tgttccgtaa cagcatgttt gtctttttgc ccagcgaagc tgacttcacg
    82441 ggcatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat tgcagccgtt
    82501 tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc ccaaatcttc
    82561 caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg gtttaccgtg
    82621 gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag cgccaccgct
    82681 tcacaggcaa tcccttcccc acgtccggta aatcccagtt tttccgtagt agtggctttc
    82741 acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg catttgtgga
    82801 atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt gccaagggta
    82861 taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct atcggcacct
    82921 ttaaatgccg gatcggtatc cgggaacagc ttgccgatat cccccagcgc cgccgcgcca
    82981 agcaatgcat cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc cagcaatcct
    83041 ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc aaaggcatgt
    83101 acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt cgggtgaggt
    83161 aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc gcacggcctt
    83221 cgaccaactg aggatggaat ccgcaatatt ccagcgccga ggcttcgtcg gtaatagtcg
    83281 cgccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg aaaaattgcg
    83341 gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca tttttgcccg
    83401 gttcggcacg tttcatagta tcgcgcactg gtgcggcgag gatccccccc gtgcggctgg
    83461 tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg caaacaagga cgagcggcgt
    83521 catgcaccaa tacccactgc gcgtcgccag cggctttcag acctgccagc acggaatcgg
    83581 cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga ggaagttgtg
    83641 caaaacggct atcgccagga cttatggcaa tgacgactcg tttcacccgg ggatgcgcca
    83701 gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga tattgcttag
    83761 gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg caaacatcca
    83821 aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc cccgcagact
    83881 gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc ctggtcatgc
    83941 tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga tcgtcaattt
    84001 cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc tgtgccgcca
    84061 catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac agcgaatact
    84121 gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg ccccctgaaa
    84181 aacggcatca tcatcccatg catccgaaga cgactctaca tcctctgttg gggataccgc
    84241 gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg gcgtgcagaa
    84301 cataatctca ttattagtta cggtttgaat tatgaacaga ggagacggga aagtacaaat
    84361 tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac cagggtgaaa
    84421 acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc caccactacc
    84481 gcgacgggca tcagcgcaag aaagaaaggc caggtgtaga taaagaagaa cagcgtgtta
    84541 gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa accgaccacg
    84601 gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa ggctgtcgtt atttgttagt
    84661 gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg ggcatggaaa
    84721 cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta ataattgctg
    84781 taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt caggcgcttc
    84841 gtaaacggaa tctattcccg taaagtagcg cagttcaccg gcacgcgctt tcttatataa
    84901 gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca cttcgataaa
    84961 gcgcccttct cctacgcgtt cacgaaccat ctggcgttcg gcgcggtgtg gcgagataaa
    85021 tgcggtcagc accaccagtc cggcttcaac catcaaattc gccacttcac cgacgcgacg
    85081 gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc cgtggcgaac
    85141 attatcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct cctccagcgc
    85201 cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca ctacaccacg
    85261 atgaccgtgg tgtagctcgc gttgttgcac agtgaccgga tggctatgcc agacgacgtt
    85321 ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca gtgtggaaag
    85381 tggcggcgaa ccagggcatt caattccagt tcgaatgcac taaattcaga tggcgcagca
    85441 gttgcctggc taactggctc atgcaccata ccggcaccta cggtcacatt gctcaggcga
    85501 tcgataaaaa tcagcccacc ggtaaccggg ttttgctgat aacgatctaa caccagtggc
    85561 tcgtcaaaag tgagatccac caggccgatg ccgttcagcg gcaggttttc aacttcgcgt
    85621 tgggtaaggt tattaatatc aacctgataa cgaatgccat caacacgagc acgcgtcttc
    85681 ttaccggcaa ttttgatgtc ataactctgg cccggggaaa gcggctgttc cgccatccat
    85741 accacatcca ccgacgcgct ctgcacagct ggtaacgctt cgtctgccgc cagcagcaga
    85801 tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc tccggcaaag
    85861 gcttcttcgc gatcgccatc aaaagtcacg atccgggcga cgtttgattc cacaccagag
    85921 ggcagcactt tgactcgttg cccgacttct acgcgaccgg atgccagcgt tccggcgtaa
    85981 ccacgaaaat cgagattcgg gcgattaacg tactgcaccg ggaagcgcat tggctgagca
    86041 tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgttgg accgctgtac
    86101 cacgccatac tttcactttg cgaagccacg ttgtcgcctt ccagtgcaga gagcggcaca
    86161 aagcggatat ccagattacc cggcagctgc ccggcaaagg tcagataatc ttcacgaata
    86221 cgggtgaacg tcttttcact gtaatccacc agatccattt tgttgatcgc cacgaccaga
    86281 tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac gggtttgatc gagcacgcct
    86341 ttacgggtat cgatcagtaa gatcgccagt tcacatgtcg atgcgccagt cgccatattg
    86401 cgggtgtact gctcgtgccc tggggtgtcg gcgataataa atttacgctt ctcggtagag
    86461 aaatagcggt aagccacgtc aatggtgatg ccctgttcgc gctcagcttg caggccgtcc
    86521 accagcagtg ccagatccag cttttcgccc tgggtgccgt gacgtttact gtcgttatgc
    86581 agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcaggcg accaatcagg
    86641 gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct tttatgttgt
    86701 tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc aagtgcggtg
    86761 ttcatcttaa aaatacccct gacgtttttt cagctccata gaccccgcct ggtcgcggtc
    86821 aatcacgcgg ccctgacgtt cactggtggt agaaaccagc atctcttcga tgatctccgg
    86881 tagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca gcgtacggaa
    86941 acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt tgtcatcaat
    87001 catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat acagcggcac
    87061 aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc agttagagag
    87121 cgggaagacg cggatgcttt cgcctttgtt aatttgcccg ttgtagttgt gccacagctc
    87181 tgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt agattcgctc
    87241 tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg aaggcggcat caaaaccgta
    87301 tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt tcgcgctgcc
    87361 gtgcacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca ccagcagctc
    87421 gcagccgtag gctttcgccg tacgatcgcg gaactcatac atctcgcgga atttccagcc
    87481 ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct tgcgcgccag
    87541 atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat ttgagaattc
    87601 tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca ggtgagtaag
    87661 tcgtatttga tccataaccg ttcctttgca ataccgctat tttcttgcca tcagatgttt
    87721 cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt agttccttaa
    87781 cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg atttcgggcg
    87841 tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg cggtaaaaaa
    87901 atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt accgctgccc
    87961 tagcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa cctggcgatt
    88021 ttgctaatac ccaggcacga catattgcta ctttctttcc gggacgcatg accggaactc
    88081 cagcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg ggttatcgca
    88141 gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat cgcaagaact
    88201 ggcataacgt gacaggaagt acggtgattg ccgctcatga aggcaaagcg ccgcagcaga
    88261 tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac gccgatgcca
    88321 atctcggcgg gctgacgtta caaggaatgg atgataacgc cgcaggttta ggtgtcatgc
    88381 tggaattggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga tttgtggcga
    88441 ccagcggcga agaggaaggg aaattaggcg ctgagaattt actcaagcgg atgagtgaca
    88501 ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt ggcgataaat
    88561 tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg cgcgacaggg
    88621 cgctggcaat tgcgcgcagt cacggaatag ccgcaacgac caatccgggt ttgaataaaa
    88681 attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa gcgggcattg
    88741 ctgtactttc ggtggaagcg actaactgga atcttgggaa taaggatggt tatcagcaac
    88801 gcgcaaaaac agccgcattc cctgcgggaa atagctggca tgacgtaaga ctggataatc
    88861 aacagcatat tgataaggca cttcctggaa gaatagaacg tcgctgccgt gacgttatgc
    88921 ggataatgct accgttggtg aaggagctgg cgaaggcgtc ttgatgggtt tgaaaatggg
    88981 agctgggagt tctaccgcag gggcggggga actccaagtg atatccatca tcgcatccag
    89041 tgcgcccggt ttatccccgc tgacgcgggg aacacacatg aatgtcggtt cagaccgtgt
    89101 ttttacccgg tttatccccg ctggcgcggg gaacacagtg ctggaccatt tcaaattcac
    89161 aatagattcg gtttatcccc gctggcgcgg ggaacacgga atgatatttc aataaataat
    89221 tataacaatc ggtttatccc cgctggcgcg gggaacacag ccgggcgaaa ttttgattca
    89281 tcgtgatgac cggtttatcc ccgctggcgc ggggaacacc ctgaacggtg gggaaaa
//
LOCUS       DBBGLQ010000009        84716 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.009, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000009 DBBGLQ010000000
VERSION     DBBGLQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.009"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            148..591
                     /gene="cmtB"
                     /locus_tag="ACIYSB_000857"
     CDS             148..591
                     /gene="cmtB"
                     /locus_tag="ACIYSB_000857"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGC5427956.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     EIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            619..2007
                     /gene="cmtA"
                     /locus_tag="ACIYSB_000858"
     CDS             619..2007
                     /gene="cmtA"
                     /locus_tag="ACIYSB_000858"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGC5427957.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            2022..3299
                     /gene="yggP"
                     /locus_tag="ACIYSB_000859"
     CDS             2022..3299
                     /gene="yggP"
                     /locus_tag="ACIYSB_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGC5427958.1"
                     /translation="MKTKVAAIYGKRDVRLRVFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3296..4261
                     /gene="glpX"
                     /locus_tag="ACIYSB_000860"
     CDS             3296..4261
                     /gene="glpX"
                     /locus_tag="ACIYSB_000860"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGC5427959.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4283..4792
                     /gene="fumE"
                     /locus_tag="ACIYSB_000861"
     CDS             4283..4792
                     /gene="fumE"
                     /locus_tag="ACIYSB_000861"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGC5427960.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHTILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFSVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4789..5502
                     /gene="yggC"
                     /locus_tag="ACIYSB_000862"
     CDS             4789..5502
                     /gene="yggC"
                     /locus_tag="ACIYSB_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGC5427961.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLTENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WLELASFCDFSIFIHAPAQILRERLISRKIAGGLTRQVAEAFYARTDGPNVERVLMNS
                     RQANLIVEMTEEGRYHFTS"
     gene            5787..6806
                     /gene="epd"
                     /locus_tag="ACIYSB_000863"
     CDS             5787..6806
                     /gene="epd"
                     /locus_tag="ACIYSB_000863"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGC5427962.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6856..8019
                     /gene="pgk"
                     /locus_tag="ACIYSB_000864"
     CDS             6856..8019
                     /gene="pgk"
                     /locus_tag="ACIYSB_000864"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGC5427963.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8234..9313
                     /gene="fbaA"
                     /locus_tag="ACIYSB_000865"
     CDS             8234..9313
                     /gene="fbaA"
                     /locus_tag="ACIYSB_000865"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGC5427964.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(9377..9475)
                     /locus_tag="ACIYSB_000866"
                     /pseudo
     CDS             complement(9377..9475)
                     /locus_tag="ACIYSB_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9671..10531
                     /gene="mscS"
                     /locus_tag="ACIYSB_000867"
     CDS             9671..10531
                     /gene="mscS"
                     /locus_tag="ACIYSB_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGC5427965.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            10670..11305
                     /gene="argO"
                     /locus_tag="ACIYSB_000868"
     CDS             10670..11305
                     /gene="argO"
                     /locus_tag="ACIYSB_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGC5427966.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VMKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLALLAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11398..12138
                     /gene="yggE"
                     /locus_tag="ACIYSB_000869"
     CDS             11398..12138
                     /gene="yggE"
                     /locus_tag="ACIYSB_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGC5427967.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12305..13201
                     /gene="ygfI"
                     /locus_tag="ACIYSB_000870"
     CDS             12305..13201
                     /gene="ygfI"
                     /locus_tag="ACIYSB_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5427968.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(13198..14676)
                     /gene="scpC"
                     /locus_tag="ACIYSB_000871"
     CDS             complement(13198..14676)
                     /gene="scpC"
                     /locus_tag="ACIYSB_000871"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGC5427969.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     VIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(14700..15485)
                     /gene="scpB"
                     /locus_tag="ACIYSB_000872"
     CDS             complement(14700..15485)
                     /gene="scpB"
                     /locus_tag="ACIYSB_000872"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGC5427970.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15496..16491)
                     /gene="meaB"
                     /locus_tag="ACIYSB_000873"
     CDS             complement(15496..16491)
                     /gene="meaB"
                     /locus_tag="ACIYSB_000873"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGC5427971.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(16484..18628)
                     /gene="scpA"
                     /locus_tag="ACIYSB_000874"
     CDS             complement(16484..18628)
                     /gene="scpA"
                     /locus_tag="ACIYSB_000874"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGC5427972.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVHQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(18832..19725)
                     /gene="argP"
                     /locus_tag="ACIYSB_000875"
     CDS             complement(18832..19725)
                     /gene="argP"
                     /locus_tag="ACIYSB_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGC5427973.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            19867..20097
                     /locus_tag="ACIYSB_000876"
     CDS             19867..20097
                     /locus_tag="ACIYSB_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGC5427974.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            20153..20812
                     /gene="rpiA"
                     /locus_tag="ACIYSB_000877"
     CDS             20153..20812
                     /gene="rpiA"
                     /locus_tag="ACIYSB_000877"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGC5427975.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21068..22300
                     /gene="serA"
                     /locus_tag="ACIYSB_000878"
     CDS             21068..22300
                     /gene="serA"
                     /locus_tag="ACIYSB_000878"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGC5427976.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22528..22587
                     /gene="ibsC"
                     /locus_tag="ACIYSB_000879"
     CDS             22528..22587
                     /gene="ibsC"
                     /locus_tag="ACIYSB_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGC5427977.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(22688..23290)
                     /gene="fau"
                     /locus_tag="ACIYSB_000880"
     CDS             complement(22688..23290)
                     /gene="fau"
                     /locus_tag="ACIYSB_000880"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGC5427978.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(23311..23494)
                     /gene="ssrS"
                     /locus_tag="ACIYSB_000881"
     ncRNA           complement(23311..23494)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACIYSB_000881"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23536..23865)
                     /gene="zapA"
                     /locus_tag="ACIYSB_000882"
     CDS             complement(23536..23865)
                     /gene="zapA"
                     /locus_tag="ACIYSB_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGC5427979.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24033..24611
                     /gene="ygfB"
                     /locus_tag="ACIYSB_000883"
     CDS             24033..24611
                     /gene="ygfB"
                     /locus_tag="ACIYSB_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGC5427980.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24637..25962
                     /gene="pepP"
                     /locus_tag="ACIYSB_000884"
     CDS             24637..25962
                     /gene="pepP"
                     /locus_tag="ACIYSB_000884"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGC5427981.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADVIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMVAARKQ"
     gene            25959..27137
                     /gene="ubiH"
                     /locus_tag="ACIYSB_000885"
                     /gene_synonym="visB"
     CDS             25959..27137
                     /gene="ubiH"
                     /locus_tag="ACIYSB_000885"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGC5427982.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFIPARDVLAQRTLGWVAR
                     "
     gene            27160..28362
                     /gene="ubiI"
                     /locus_tag="ACIYSB_000886"
                     /gene_synonym="visC"
     CDS             27160..28362
                     /gene="ubiI"
                     /locus_tag="ACIYSB_000886"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGC5427983.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVQEPLAANA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAHQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFASHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(28436..28519)
                     /locus_tag="ACIYSB_000887"
     CDS             complement(28436..28519)
                     /locus_tag="ACIYSB_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5427984.1"
                     /translation="MSKNTKSKNNGIRKYNAKTEVKLVYFK"
     gene            28810..29904
                     /gene="gcvT"
                     /locus_tag="ACIYSB_000888"
     CDS             28810..29904
                     /gene="gcvT"
                     /locus_tag="ACIYSB_000888"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGC5427985.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            29928..30317
                     /gene="gcvH"
                     /locus_tag="ACIYSB_000889"
     CDS             29928..30317
                     /gene="gcvH"
                     /locus_tag="ACIYSB_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGC5427986.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            30436..33309
                     /gene="gcvP"
                     /locus_tag="ACIYSB_000890"
     CDS             30436..33309
                     /gene="gcvP"
                     /locus_tag="ACIYSB_000890"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGC5427987.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            33576..34319
                     /gene="ygfF"
                     /locus_tag="ACIYSB_000891"
     CDS             33576..34319
                     /gene="ygfF"
                     /locus_tag="ACIYSB_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGC5427988.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(34376..35809)
                     /gene="bglA"
                     /locus_tag="ACIYSB_000892"
     CDS             complement(34376..35809)
                     /gene="bglA"
                     /locus_tag="ACIYSB_000892"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGC5427989.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            35854..36165
                     /gene="yqfB"
                     /locus_tag="ACIYSB_000893"
     CDS             35854..36165
                     /gene="yqfB"
                     /locus_tag="ACIYSB_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGC5427990.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            36329..36988
                     /gene="yqfA"
                     /locus_tag="ACIYSB_000894"
     CDS             36329..36988
                     /gene="yqfA"
                     /locus_tag="ACIYSB_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGC5427991.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(37184..38164)
                     /gene="ygfZ"
                     /locus_tag="ACIYSB_000895"
     CDS             complement(37184..38164)
                     /gene="ygfZ"
                     /locus_tag="ACIYSB_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGC5427992.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            38407..38673
                     /gene="sdhE"
                     /locus_tag="ACIYSB_000896"
     CDS             38407..38673
                     /gene="sdhE"
                     /locus_tag="ACIYSB_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGC5427993.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            38654..39061
                     /gene="ygfX"
                     /locus_tag="ACIYSB_000897"
     CDS             38654..39061
                     /gene="ygfX"
                     /locus_tag="ACIYSB_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGC5427994.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(39101..39622)
                     /gene="fldB"
                     /locus_tag="ACIYSB_000898"
     CDS             complement(39101..39622)
                     /gene="fldB"
                     /locus_tag="ACIYSB_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGC5427995.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            39734..40630
                     /gene="xerD"
                     /locus_tag="ACIYSB_000899"
     CDS             39734..40630
                     /gene="xerD"
                     /locus_tag="ACIYSB_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGC5427996.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            40655..41365
                     /gene="dsbC"
                     /locus_tag="ACIYSB_000900"
     CDS             40655..41365
                     /gene="dsbC"
                     /locus_tag="ACIYSB_000900"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGC5427997.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQQ
                     PKEMKEFLDEHQKMTSGK"
     gene            41371..43104
                     /gene="recJ"
                     /locus_tag="ACIYSB_000901"
     CDS             41371..43104
                     /gene="recJ"
                     /locus_tag="ACIYSB_000901"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGC5427998.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFKLFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            43195..44293
                     /gene="prfB"
                     /locus_tag="ACIYSB_000902"
     CDS             join(43195..43269,43271..44293)
                     /gene="prfB"
                     /locus_tag="ACIYSB_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGC5427999.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            44303..45820
                     /gene="lysS"
                     /locus_tag="ACIYSB_000903"
     CDS             44303..45820
                     /gene="lysS"
                     /locus_tag="ACIYSB_000903"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGC5428000.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTKLRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(45864..46412)
                     /gene="idi"
                     /locus_tag="ACIYSB_000904"
     CDS             complement(45864..46412)
                     /gene="idi"
                     /locus_tag="ACIYSB_000904"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGC5428001.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            46467..46538
                     /locus_tag="ACIYSB_000905"
     CDS             46467..46538
                     /locus_tag="ACIYSB_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428002.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(46535..46660)
                     /gene="yqfG"
                     /locus_tag="ACIYSB_000906"
     CDS             complement(46535..46660)
                     /gene="yqfG"
                     /locus_tag="ACIYSB_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGC5428003.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(46662..48110)
                     /gene="uacT"
                     /locus_tag="ACIYSB_000907"
     CDS             complement(46662..48110)
                     /gene="uacT"
                     /locus_tag="ACIYSB_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGC5428004.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            48546..50465
                     /gene="ygfT"
                     /locus_tag="ACIYSB_000908"
     CDS             48546..50465
                     /gene="ygfT"
                     /locus_tag="ACIYSB_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGC5428005.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            50465..50953
                     /gene="ygfS"
                     /locus_tag="ACIYSB_000909"
     CDS             50465..50953
                     /gene="ygfS"
                     /locus_tag="ACIYSB_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGC5428006.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(50989..52356)
                     /gene="ghxQ"
                     /locus_tag="ACIYSB_000910"
                     /gene_synonym="ygfQ"
     CDS             complement(50989..52356)
                     /gene="ghxQ"
                     /locus_tag="ACIYSB_000910"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGC5428007.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(52392..53708)
                     /gene="guaD"
                     /locus_tag="ACIYSB_000911"
     CDS             complement(52392..53708)
                     /gene="guaD"
                     /locus_tag="ACIYSB_000911"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGC5428008.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTIDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(53726..55126)
                     /gene="xanQ"
                     /locus_tag="ACIYSB_000912"
     CDS             complement(53726..55126)
                     /gene="xanQ"
                     /locus_tag="ACIYSB_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGC5428009.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(55291..58161)
                     /gene="xdhD"
                     /locus_tag="ACIYSB_000913"
     CDS             complement(55291..58161)
                     /gene="xdhD"
                     /locus_tag="ACIYSB_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGC5428010.1"
                     /translation="MIINFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(58158..58937)
                     /gene="ygfM"
                     /locus_tag="ACIYSB_000914"
     CDS             complement(58158..58937)
                     /gene="ygfM"
                     /locus_tag="ACIYSB_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGC5428011.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(58988..60316)
                     /gene="ssnA"
                     /locus_tag="ACIYSB_000915"
     CDS             complement(58988..60316)
                     /gene="ssnA"
                     /locus_tag="ACIYSB_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGC5428012.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(60319..63417)
                     /gene="ygfK"
                     /locus_tag="ACIYSB_000916"
     CDS             complement(60319..63417)
                     /gene="ygfK"
                     /locus_tag="ACIYSB_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGC5428013.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTIEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLLVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(63739..64317)
                     /gene="mocA"
                     /locus_tag="ACIYSB_000917"
     CDS             complement(63739..64317)
                     /gene="mocA"
                     /locus_tag="ACIYSB_000917"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGC5428014.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            64420..65190
                     /gene="yqeC"
                     /locus_tag="ACIYSB_000918"
     CDS             64420..65190
                     /gene="yqeC"
                     /locus_tag="ACIYSB_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGC5428015.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            65238..66863
                     /gene="yqeB"
                     /locus_tag="ACIYSB_000919"
     CDS             65238..66863
                     /gene="yqeB"
                     /locus_tag="ACIYSB_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGC5428016.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(67084..68016)
                     /gene="arcC"
                     /locus_tag="ACIYSB_000920"
     CDS             complement(67084..68016)
                     /gene="arcC"
                     /locus_tag="ACIYSB_000920"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGC5428017.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(68064..69449)
                     /gene="hyuA"
                     /locus_tag="ACIYSB_000921"
     CDS             complement(68064..69449)
                     /gene="hyuA"
                     /locus_tag="ACIYSB_000921"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGC5428018.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(69502..70713)
                     /gene="ygeY"
                     /locus_tag="ACIYSB_000922"
     CDS             complement(69502..70713)
                     /gene="ygeY"
                     /locus_tag="ACIYSB_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGC5428019.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(70771..71967)
                     /gene="dpaL"
                     /locus_tag="ACIYSB_000923"
     CDS             complement(70771..71967)
                     /gene="dpaL"
                     /locus_tag="ACIYSB_000923"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGC5428020.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPGKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(72025..73212)
                     /gene="ygeW"
                     /locus_tag="ACIYSB_000924"
     CDS             complement(72025..73212)
                     /gene="ygeW"
                     /locus_tag="ACIYSB_000924"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGC5428021.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            73691..75469
                     /gene="ygeV"
                     /locus_tag="ACIYSB_000925"
     CDS             73691..75469
                     /gene="ygeV"
                     /locus_tag="ACIYSB_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGC5428022.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFNQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(75509..75988)
                     /gene="xdhC"
                     /locus_tag="ACIYSB_000926"
     CDS             complement(75509..75988)
                     /gene="xdhC"
                     /locus_tag="ACIYSB_000926"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGC5428023.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(75985..76863)
                     /gene="xdhB"
                     /locus_tag="ACIYSB_000927"
     CDS             complement(75985..76863)
                     /gene="xdhB"
                     /locus_tag="ACIYSB_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGC5428024.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(76874..79171)
                     /gene="xdhA"
                     /locus_tag="ACIYSB_000928"
     CDS             complement(76874..79171)
                     /gene="xdhA"
                     /locus_tag="ACIYSB_000928"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGC5428025.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVAHDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            79586..80341
                     /gene="actS"
                     /locus_tag="ACIYSB_000929"
     CDS             79586..80341
                     /gene="actS"
                     /locus_tag="ACIYSB_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGC5428026.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSITRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            80420..80493
                     /locus_tag="ACIYSB_000930"
     tRNA            80420..80493
                     /locus_tag="ACIYSB_000930"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80452..80454,aa:Gly,seq:ccc)
     gene            80649..81443
                     /gene="ygeQ"
                     /locus_tag="ACIYSB_000931"
     CDS             80649..81443
                     /gene="ygeQ"
                     /locus_tag="ACIYSB_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HGC5428027.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGIPVQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAINTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDS"
     gene            81510..82259
                     /locus_tag="ACIYSB_000932"
     CDS             81510..82259
                     /locus_tag="ACIYSB_000932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428028.1"
                     /translation="MSNLLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYER
                     ITLQLDLGGESPGYLEKDKHYREADAALLNVIYPTNLSKINTRRKEQVLKIVKKLAGP
                     YGIKRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIV
                     YKESRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEA
                     PSPIIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            82272..82391
                     /locus_tag="ACIYSB_000933"
                     /pseudo
     CDS             82272..82391
                     /locus_tag="ACIYSB_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            82351..83338
                     /locus_tag="ACIYSB_000934"
                     /pseudo
     CDS             82351..83338
                     /locus_tag="ACIYSB_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            83328..83993
                     /locus_tag="ACIYSB_000935"
     CDS             83328..83993
                     /locus_tag="ACIYSB_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGC5428029.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVNFFEKIQKVNSSEDNEEIFDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            84003..84263
                     /locus_tag="ACIYSB_000936"
     CDS             84003..84263
                     /locus_tag="ACIYSB_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGC5428030.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            84265..>84716
                     /gene="sctT"
                     /locus_tag="ACIYSB_000937"
     CDS             84265..>84716
                     /gene="sctT"
                     /locus_tag="ACIYSB_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HGC5428031.1"
                     /translation="MGEAILYQLHSLLAATALCFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETL"
BASE COUNT        20853 a        20662 c        22178 g        21023 t
ORIGIN      
        1 tatatctata actcaatgtt atataagaaa ttaataattc actgttttca aaacaccggt
       61 ttccctgctc aattgctttc attaaaccgc acgagtattt tatttttacc gccggacggt
      121 accctaaaaa taaaataagg ataatttatg cggcttagtg attattttcc agaatcatca
      181 atctcagtta tacattcagc aaaagattgg caggaagcta tcgatttctc gatggtatca
      241 ttgctggata aaaactatat cagcgagaat tacattcagg aaattaaaga ttccaccatt
      301 aacaatggcc cttattatat tctcgcacca ggcgtggcaa tgcctcatgc gcgaccggaa
      361 tgtggggcgc ttaaaaccgg gatgtctttg acattacttg aacaaggtgt ttattttccg
      421 gggaatgacg aaccaattaa attactcatc ggactctctg ctgccgatgc cgattcgcac
      481 attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag aagaaatact cgaacaactc
      541 ttaacagcat catcagaaaa acaattagcg gacattatca gccgcggata attactctct
      601 cctctacagg ataatattat ggaaaacaag tctgctcgtg caaaggtcca ggcttttggg
      661 ggctttttga ctgcaatggt catccccaat attggtgctt ttattgcctg gggttttatt
      721 actgcgttat ttattcccac cggttggctg cctaacgaac atttcgccaa aattgtcggc
      781 ccgatgatta cctatttatt gcccgtgatg attggttcta caggtggtca tctggtcggc
      841 ggtaaacgcg gggcggtcat gggcggaata ggtactattg gtgtgatcgt tggcgcagag
      901 atcccgatgt tccttggctc aatgatcatg gggccgctcg gtgggttggt cataaaatat
      961 gtcgataagg cactggaaaa acgcatacct gccggttttg agatggttat caataacttc
     1021 tcattaggta tcgcggggat gctcctttgt ctgctgggtt ttgaagttat cggcccggcg
     1081 gtgttaattg ccaatacttt cgtcaaagag tgtattgagg cgctggtaca tgcgggttat
     1141 ctgccattgt tgtcagtcat caatgaaccg gcgaaagtgc ttttcctcaa taatgcgatc
     1201 gatcagggcg tctattatcc gctgggaatg caacaggctt cggttaacgg taaatccatc
     1261 ttctttatgg tggcctctaa cccaggtccg ggcctggggc tgctgctggc gtttaccttg
     1321 tttggtaaag ggatgagtaa acgttctgcg cccggggcga tgattattca cttcctcggt
     1381 gggatccacg aactgtattt cccgtatgtg ctgatgaagc cgctgaccat tattgccatg
     1441 attgcgggcg gtatgtctgg cacctggatg tttaacttac tggacggtgg tctggtggct
     1501 ggcccaagtc cggggtctat ctttgcttac ctggcactga cgccgaaagg ctcgttcctg
     1561 gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct ttgctattac ttcgctgata
     1621 ctgaagatgg aaaaaacggt ggaaacggag agcgaagatg agtttgctca gtcagccaat
     1681 gcggttaagg cgatgaaaca agagggtgca ttctcgttaa gcagggttaa gcgtatcgcc
     1741 tttgtttgcg atgcggggat gggctccagt gcgatgggcg cgaccacctt ccgtaaacgc
     1801 ctggaaaaag cggggctggc aattgaagta aaacattacg ccatagaaaa cgtgcctgcg
     1861 gatgcggata tcgtcgttac tcatgccagt ctggaagggc gcgtgaaacg tgtgacggat
     1921 aaaccactga tattgattaa taactatatt ggcgatccaa aactcgacac tttatttaat
     1981 caattaaccg ccgaacataa acactgatta cagaggtaaa aatgaaaacc aaagttgctg
     2041 ctatttatgg caagcgggat gtccgtctgc gcgtatttga actgccagaa attaccgata
     2101 atgaattact ggtgagtgta atttctgaca gcgtctgttt atcgacctgg aaagcggcgt
     2161 tactcggtag tgaacataaa cgcgtacccg acgatttaga aaatcatccg gtcattaccg
     2221 ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa tctcactggc aaatataaaa
     2281 aaggccagcg ttttgtattg caaccggcga tggggttacc aagcggatat tcagcgggct
     2341 acagctacga atattttggc ggcaatgcca cttatatgat tattcccgaa atcgccatta
     2401 atttgggctg cgtattaccg tatcacggct cttattttgc tgcggcgtcg ctggcagagc
     2461 ctatgtgctg cattattggt gcttatcatg ccaattatca caccacgcaa tatgtttatg
     2521 agcatcgcat gggcgtcaaa cctggcggca atattgcact gctggcgtgt gcaggtccga
     2581 tgggcattgg cgctatcgat tacgccatta acggcggcat acaaccgtcg cgggtggtgg
     2641 tggtcgatat cgacgacaaa cgtctggcgc aggtacagaa gctgctgccg gtggaactag
     2701 cggccagtaa aggcattgag ctggtgtatg tgaataccaa agggatgagc gatcctgtcc
     2761 agatgctgcg ggcgctgaca ggagatgccg ggttcgatga catttttgtt tatgcggcgg
     2821 tgcctgctgt cgttgagatg gctgatgaat tactggcgga agatggctgt ctgaacttct
     2881 ttgccgggcc gacggataaa aacttcaaag tgccgtttaa tttctacaac gtccattaca
     2941 acagcacgca cgtcgtcggt acatctggcg gttcaacgga cgacatgaaa gaggcgattg
     3001 cccttagcgc cactgggcag ttacagccgt cgtttatggt gacccatatc ggtggcctgg
     3061 atgcggtgcc agaaaccgtg ctcaatctgc cggatatccc tggcggtaaa aaactcattt
     3121 ataacggcgt gaccatgccg ctcactgcca ttgccgattt tgccgaaaaa ggcaaaaccg
     3181 atccgctgtt taaagagttg gcgcggctgg ttgaggaaac gcacggcatc tggaatgaac
     3241 aggccgagaa atatctgctg gcacaatttg gcgttgatat cggggaggcc gcgcaatgat
     3301 gtccctggcg tggccattat ttcgcgttac ggaacaggca gcgttggctg cctggccgca
     3361 aaccggatgt ggcgacaaaa ataaaattga tggcctggcg gtcaccgcga tgcgccaggc
     3421 attaaacgac gtcgcttttc gtggacgagt ggttatcggc gaaggagaaa ttgaccatgc
     3481 gccgatgctg tggattggcg aagaggtggg caaaggcgat gggccagaag tcgatatcgc
     3541 ggttgatccc attgaaggca cgcggatggt ggcgatgggg cagagcaatg cgctggcggt
     3601 catggctttc gcccctcgag atagcctgtt acatgcgccc gatatgtata tgaaaaagct
     3661 ggttgttaat cggctggcgg cgggcgcgat tgatctgtct ttgcctctga cggacaacct
     3721 gcgcaacgtg gcgaaagcgt taggcaaacc gctggataag ctgcgcatgg ttacgctgga
     3781 taaaccgcgc ctgagtgccg cgattgagga agcaacacag ctgggcgtga aggtttttgc
     3841 cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc tggcaggata atccttacga
     3901 tgtgatgtac accatcggcg gtgcgccaga aggcgtgatt tctgcctgtg ccgttaaagc
     3961 attaggcggg gatatgcagg ccgagctgat tgacttttgc caggctaaag gggattacac
     4021 ggaaaatcgg cagattgcag agcaggagcg caagcgttgc aaggcaatgg gcgttgacgt
     4081 taaccgtgtg tactcgctcg atgaactggt gagggggaac gatatcctct ttagcgctac
     4141 gggcgtgacg ggcggtgagc tggtgaacgg tatccaacag acggcgaatg gggtgcggac
     4201 gcagacatta ctgatcggcg gcgcggacca aacgtgtaat ataatagact ccctgcattg
     4261 atggtgatct atcggaagac caatggcgac gctgacagaa gatgatgtgc ttgagcaact
     4321 ggatgcacag gacaatttat tttcatttat gaaaactgcg cataccattt tgctccaggg
     4381 gatacgccag tttctgccgt cgctgtttgt cgataacgat gaagagatcg tggaatatgc
     4441 agtgaagccg ttactcgccc aaagcggccc gcttgacgat attgatgttg cactgcgttt
     4501 gatttatgcg ttggggaaaa tggataaatg gttgtacgcc gatatcacgc atttttccca
     4561 gttctggcat tacctgaacg aacaggatga aacgcccgga tttgccgatg acatgacctg
     4621 ggattttatc agcaacgtca atagcataac ccgcaatgcg atgctctatg acgcgttaaa
     4681 agcgatgaag ttcgctgatt tctccgtctg gtcagaggcg cgttttagcg gaatggtcaa
     4741 aacggcgctg acgctggcag taacgaccac cttaaaggaa ttaacgccgt gaaaattgaa
     4801 ttaacggtga atgggctgaa gatacaggca cagtaccaga acgaagagat tgaaaatgta
     4861 cataaaccgt tgctgcacat gttggcggcc ttacagacgg taaatccgca gcggcgtacg
     4921 gtggtttttc tttgcgctcc gccggggacg ggtaaatcaa ccctaaccac cttctgggaa
     4981 tatctcgcgc aacaagaccc ggaactgccc gctattcaga cgctcccgat ggatggtttt
     5041 caccattaca atagctggct ggatgcgcat caattgcgcc ccttcaaagg cgcaccagag
     5101 acatttgacg ttgcgaaact gacggaaaat ctgcgccagg tcgtggaagg ggattgtacg
     5161 tggccgcagt acgatcgaca aaagcatgat cctgttgaag atgcgttgca cgttaccgca
     5221 ccactcgtca tcgtcgaagg aaactggttg ttactggatg atgaaaagtg gctggagttg
     5281 gcatctttct gcgatttttc tatttttatt catgcacctg ctcaaattct gcgggagcgc
     5341 ttgattagtc gtaaaattgc gggaggatta acgcggcaag tagccgaagc attttatgcc
     5401 cgcaccgatg gccccaatgt cgagcgtgtg ctgatgaata gccgtcaggc caatctgata
     5461 gtagagatga ctgaagaagg ccggtatcac ttcacaagct gacaatttat tccagttatt
     5521 gtagggttat tattacgcca gcctggaata aagcttccca caaaagggga taaaagtgtg
     5581 atgtgagtca gataaatgtc ttcttcggct ggacaaacat tccttttatt ccacgtttcg
     5641 cttatcctag ctgaagcgtt tcagtcgatt aaatgttcga caattaacca atcagtcgca
     5701 gtttgcgaca ggtaaggttt ccccggacga tttgctggat tactctgtct ggcaaatttg
     5761 ctcagggaaa accttgcagg agatctatga ccgtacgcgt agcgataaat ggcttcggtc
     5821 gcatcgggcg taatgtggtt cgtgctttgt atgaatccgg acgccgggcg gaaattaccg
     5881 tggtggcaat caacgaactg gcggatgctg cgggcatggc gcatttgttg aaatatgaca
     5941 ccagccatgg ccgttttgca tgggaagtac gacaggaacg cgatcaactt tttgttggtg
     6001 atgacgccat ccgcgtattg catgaacgtt cactgcaatc gctcccctgg cgtgaacttg
     6061 gcgttgatgt agtcctcgac tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc
     6121 atattgccgc cggggccaaa aaagtgctct tttcacatcc tggcagtaac gatctcgacg
     6181 cgaccgttgt ttacggcgtc aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta
     6241 acgcttcctg taccacgaat tgcataattc ccgtcatcaa attgttagat gatgcgtacg
     6301 gtattgagtc cggcactgtg accacaattc actccgccat gcacgatcaa caggttattg
     6361 atgcatacca tcctgacctg cgtcgcaccc gggcagccag ccagtcgatc attccggtcg
     6421 atactaaact ggccgccggt atcacacgat tttttccgca atttaacgat cgctttgaag
     6481 cgattgcggt acgtgtgcca accataaatg tgacggcaat cgatttaagc gtgacggtga
     6541 agaaacctgt aaaagccaat gaagtcaacc tgttgctgca aaaagcagca caaggtgcat
     6601 ttcatggtat agttgactat acggaattgc cgttggtctc tgtagatttt aaccacgatc
     6661 cgcacagtgc cattgtcgat ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa
     6721 cgttggtctg gtgcgataac gaatggggct ttgctaaccg aatgctcgac acgacgttag
     6781 ctatggctac tgttgctttc aggtaagacg caagcagcgt ctgcaaaact tttagaatca
     6841 acgagaggat tcaccatgtc tgtaattaag atgaccgatc tggatcttgc tgggaaacgt
     6901 gtatttatcc gtgcggatct gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg
     6961 cgtatccgtg cttctctgcc gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg
     7021 gtaacttccc acctgggtcg tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg
     7081 ccggttgtta actacctgaa agacaaactg tctaacccgg ttcgtctggt taaagattac
     7141 ctcgacggcg ttgacgttgc tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac
     7201 aaaggcgaga agaaagacga cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg
     7261 ttcgtaatgg acgcattcgg tactgctcac cgcgcgcagg cttctactca cggtatcggt
     7321 aaattcgctg acgttgcgtg cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt
     7381 aaagcactga aagaacctgc tcgcccgatg gtggctatcg ttggtggttc taaagtatct
     7441 accaaactga ccgttctgga ctccctgtct aaaatcgctg accagctgat tgttggtggt
     7501 ggtatcgcta acacctttat cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa
     7561 gctgacctgg ttgacgaagc taaacgtctg ctgaccacct gcaacatccc ggttccgtct
     7621 gatgttcgcg tagcaaccga gttctctgaa actgcaccgg ctaccctgaa atctgttaac
     7681 gatgtgaaag ctgacgagca gatcctggat atcggtgatg cttccgctca ggaactggct
     7741 gaaatcctga agaatgcgaa aaccattctg tggaacggtc cggttggcgt gttcgaattc
     7801 ccgaacttcc gcaaaggtac tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc
     7861 tccatcgctg gcggcggcga cactctggca gcaatcgacc tgttcggcat tgctgacaaa
     7921 atctcctaca tctccactgg cggcggcgca ttcctcgaat tcgtggaagg taaagtactg
     7981 cctgcagtag cgatgctcga agagcgcgct aagaagtaaa aaatcacagg gcagggaaac
     8041 ctgcccttgt ttcagcgcgc ttttagagca cgcaattttt catactctaa ataattcgag
     8101 ttgcaggaag gcgacaagag agtgaatccc caggagctta cataagtaag tgactggggt
     8161 gagcgaacgc agacgcagca catgcaactt gaagtatgac gagtataagg cccgacgata
     8221 caggacaaga gacatgtcta agatttttga tttcgtaaaa cctggcgtaa tcactggtga
     8281 tgacgtacag aaagttttcc aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa
     8341 ctgcgtcggt actgactcca tcaacgccgt actggaaacc gctgctaaag ttaaagcgcc
     8401 ggttatcgtt cagttctcca acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc
     8461 tgacgttccg cagggtgctg ctatcctggg cgcgatctct ggtgcgcatc acgttcacca
     8521 gatggctgaa cattatggtg ttccggttat cctgcacact gaccactgcg cgaagaaact
     8581 gctgccgtgg atcgacggtc tgttggacgc gggtgaaaaa cacttcgcag ctaccggtaa
     8641 gccgctgttc tcttctcaca tgatcgacct gtctgaagaa tctctgcaag agaacatcga
     8701 aatctgctct aaatacctgg agcgcatgtc caaaatcggc atgactctgg aaatcgaact
     8761 gggttgcacc ggtggtgaag aagacggcgt ggacaacagc cacatggacg cttctgcact
     8821 gtacacccag ccggaagacg ttgattacgc atacaccgaa ctgagcaaaa tcagcccgcg
     8881 tttcaccatc gcagcgtcct tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt
     8941 tctgactccg accatcctgc gtgattctca ggaatatgtt tccaagaaac acaacctgcc
     9001 gcacaacagc ctgaacttcg tattccacgg tggttccggt tctactgctc aggaaatcaa
     9061 agactccgta agctacggcg tagtaaaaat gaacatcgat accgataccc aatgggcaac
     9121 ctgggaaggc gttctgaact actacaaagc gaacgaagct tatctgcagg gtcagctggg
     9181 taacccgaaa ggcgaagatc agccgaacaa gaaatactac gatccgcgcg tatggctgcg
     9241 tgccggtcag acttcgatga tcgctcgtct ggagaaagca ttccaggaac tgaacgcgat
     9301 cgacgttctg taagatattc ctttctgctt atctcaaggc ccgctctgcg ggtctttttt
     9361 tcgccaaaag caaaattgcc tgatgcgcta cgcttatcag gcctacacgt atcctgcaat
     9421 atattgaatt tgcaggtttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgac
     9481 aaagcgcaca ttgatatctg tctgagcccc gctctgcggg gattttttcg ccaaaagcaa
     9541 ggtgattcag atgagaaatc tgtgatctat ttggcaaaat tatgctttat tgtttaccct
     9601 tgtcagactg cccgtcataa ggcggcggag tgtatttctc cattttgagt cagttgaaaa
     9661 ggaatattga atggaagatt tgaatgttgt cgatagcata aacggcgcgg gaagctggct
     9721 ggtagctaac caggcgctgc tgctaagtta tgcagtaaac atcgtggcgg cactcgcgat
     9781 catcatcgtt ggtttgatta tcgcgcggat gatttccaac gcggtgaatc gcctgatgat
     9841 ctcccgtaaa atcgatgcca ctgttgctga ttttctttct gcattagtcc gttacggtat
     9901 tatcgccttt acgctaatcg ctgcactggg acgcgtgggt gtacaaaccg cgtcagtcat
     9961 tgctgtactc ggtgccgcag gcttagctgt tggtctggct ttgcaggggt cactgtctaa
    10021 cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc cgtgccggag aatatgttga
    10081 cctgggcggc gtagccggta ctgtgctgag tgtgcagatt ttctccacca ccatgcgtac
    10141 tgcagacggt aaaattatcg ttattccgaa cggtaaaatt attgccggaa atattattaa
    10201 cttctcccgc gagccagttc gccgtaacga atttattatt ggcgtggcgt atgattccga
    10261 tatcgatcag gttaagcaga tcctgaccaa tattatccag tctgaagatc gcattttgaa
    10321 agatcgcgaa atgactgtgc gcctgaacga acttggtgca tcgtcgatta atttcgtggt
    10381 ccgcgtctgg agcaacagcg gcgatctgca aaacgtgtac tgggatgtgc tggagcgtat
    10441 taaacgtgaa tttgatgccg ccggtatcag cttcccgtac ccgcaaatgg atgtgaactt
    10501 taagcgggtg aaagaagaca aagctgcgta atcaacgctg aggccagata atactctctg
    10561 gcctctctct tattagtttt tctgattgcc aattaatatt atcaatttcc gctaataaca
    10621 atcccgcgat atagtctctg catcagatac ttaattcgga atatccaacg tgttttctta
    10681 ttactttcaa ggtcttgcac ttggggcggc tatgatccta ccgctcggtc cacaaaatgc
    10741 ttttgtgatg aatcagggca tacgtcgtca gtaccacatt atgattgcct tactttgtgc
    10801 tatcagcgat ttggtcctga tttgcgccgg gatttttggt ggcagcgcgt tattgatgca
    10861 gtcgccgtgg ttgctggcgc tggtcacctg gggcggcgta gccttcttgc tgtggtatgg
    10921 ttttggcgct tttaaaacag caatgagcag taatattgag ttagccagcg ccgaagtcat
    10981 gaagcaaggc agatggaaaa ttatcgccac catgttggca gtgacctggc tgaatccgca
    11041 tgtttacctg gatacttttg ttgtactggg cagccttggc gggcaacttg atgtggaacc
    11101 aaaacgctgg tttgcactcg ggacaattag cgcctctttc ctgtggttct ttggtctggc
    11161 tcttctcgca gcctggctgg caccgcgtct gcgcacggca aaagcacagc gcattatcaa
    11221 tctggttgtg ggatgtgtta tgtggtttat tgccttgcag ctggcgagag acggtattgc
    11281 tcatgcacaa gccttgttca gttaggcgct atctgatgga aaaataaaac agaggcgcta
    11341 agcttgcctc cagaggtcct gaattttggg caagtcaatt aacacggaga gactaacgtg
    11401 aagttcaaag ttatcgccct ggcggcatta atgggtatta gcgggatggc agcgcaggct
    11461 aacgaattgc cggatggacc gcatattgtc acctccggta cggcaagcgt ggatgcggtg
    11521 ccagacattg ccactcttgc gattgaagtt aacgtggccg cgaaggatgc cgctactgcc
    11581 aagaaacagg cagatgagcg cgtcgcacaa tacatttcct tccttgaact caatcagatc
    11641 gcgaaaaaag atatcagctc agcgaactta cgcacccagc cagattatga ttatcaggat
    11701 ggtaaaagta tccttaaagg ctaccgcgct gtgagaacgg tggaagtcac gctccgtcag
    11761 ttagacaaac tgaattcctt gctggatggc gcgctgaagg cgggtcttaa cgaaattcgt
    11821 tctgtgtcgc tgggcgtggc gcagccggat gcctataaag acaaagcgcg taaggcagcg
    11881 attgataacg cgattcatca ggcgcaggaa ctggcgaacg gctttcatcg taaactgggg
    11941 ccggtatata gcgtgcgcta ccatgtttcc aactatcagc ccagcccaat ggtgcggatg
    12001 atgaaagccg atgccgcgcc ggtgtccgcc caggaaactt acgagcaggc cgctattcag
    12061 tttgatgatc aggtcgatgt ggtcttccag ttagaacctg tggatcaaca acccgctaaa
    12121 acacctgcag cacaataata tttgtagccc gacgtattcg tcgggctaat gcctttaccc
    12181 gatattgcct cctttttctg taattttctg taaaaccatg acgtaatcac ctatctttgc
    12241 tcgcggtttc atcaaggata ggctgactga aacggttaag cggacaagga tgcgggggag
    12301 ggggatggac atttttattt cgaaaaagat gcgcaatttc attttattag cgcaaaccaa
    12361 taatattgcc cgggcggcag aaaaaatcca tatgacggct tcgccatttg gtaaaagcat
    12421 tgccgcgctg gaagaacaaa ttggctatac gctatttacc cgcaaagata ataatatcag
    12481 cctcaataaa gccggacagg aactgtatca aaaactgttc ccggtttatc aacggctttc
    12541 tgcgattgat aatgaaatcc ataactcagg gcgtcgttca cgagaaattg tgattggcat
    12601 agataatact tatccgacaa ttattttcga tcagttgatt agtctcggag ataagtatga
    12661 aggcgtgacc gcccagccag ttgagttcag cgaaaatggc gttatcgata atctttttga
    12721 ccgccagtta gattttatta tctccccaca gcatgtatct gcacgagttc aggaactgga
    12781 aaacctgact atcagtgaac taccaccgct gcgattaggc tttctcgttt ctcgccgtta
    12841 tgaagagcga caagagcaag agctgcttca ggagctgccc tggctacaaa tgcgctttca
    12901 gaaccgggcc aacttcgaag caatgattga tgcgaatatg cgaccttgtg ggatcaatcc
    12961 aaccattatt tatcgtccct acagttttat ggccaaaatc agcgccgtgg agcgcggaca
    13021 ttttctgacg gtgatcccgc attttgcctg gcgactggtg aatccggcaa cgctgaaata
    13081 ttttgatgca ccgcacaggc cgatgtatat gcaggaatat ctttattcaa tcagaaatca
    13141 tcgttatacc gccacgatgc ttcagcatat tgctgaagat cgtgacggga cgagtcatta
    13201 acccagcatc gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc
    13261 gtggtgaata tgtccgccag gcgcattttc cagatagcga tgcagataat ctcgatacat
    13321 aggatgtgca caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag
    13381 atcggcgatc ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt gatcaacatg
    13441 gctgcacatt ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gggccataaa
    13501 gatcgacaga tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc
    13561 gaccccggct acgtgtgttg agttggcatg cccgtaaata tcaaactcca ggccgacgtt
    13621 cagagcgatg acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg
    13681 caacacaatg cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc
    13741 cgagattgtc aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac
    13801 caccgattcc tgtagcactt ccgaatacat cataaacgga ggaattaccg ggttttcccc
    13861 cagacgcgcc attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc
    13921 cggcggaata cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat
    13981 ctgctggcac atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc
    14041 cacgacggcg acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat
    14101 tgcatggaag atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatccgc
    14161 cagttctgca acgcgcggat cgtgatagtg attgagttca atgatcactt tcttcgcccg
    14221 cagcagccag gtcggcgcat taccgatccc gctggttaac cagactcgac catccggtgc
    14281 cagtgccgat gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat
    14341 ttgcgccact tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt
    14401 acgtaaaccg gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag
    14461 tacatcgtca gcggcggcgc tgattgacgc acccgtcaga aggcgaattt gatacggctt
    14521 tttggcctca tgctgttcgt tagctctgcg ggcaatcgcg gtgggtaggg ctttcggcga
    14581 acccgccggg gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc
    14641 ttcattggcg gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat
    14701 taatgaccaa cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa
    14761 tcttcgctgt catacaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc
    14821 atggtgtgtg cttcgcccag tacacgcagc tcttctttga taacggcaat ggctaacggc
    14881 gctttctcag agatgtggtg cgccatttgt aaggtgaaat cttccagttc ttccacttcc
    14941 acaacatggt tgaggatgcc gacagccagc gcgcgctggg cggtgattgg cgaagcggta
    15001 aaaatcagct ctttgacaat gtggaagccc gcgtcgcggg tcaggttgtg aatgccgacc
    15061 aggttatacg ggacgccgag gtttacaggc gtcattgaga aggttgaggt actggcggcg
    15121 atgatcagat cggaactcat gatcatttca aatgcgccac cccaaacact accttccacc
    15181 atcgaaatga tcggtttcgg gaatttttgg atcatgcggg tgatttgacg caatggatca
    15241 tcataggaga gcggatcgcg accgccagac ggcagttcgt gaatatcgtg acctgcggag
    15301 aagactttgg atccactcgg tgcgcgcaaa atgatacagc gaatttccgg ccggttgaga
    15361 tcgcttaacg cctgcataag atcatcaata aagactttac ttaaggcatt aagttttcgg
    15421 ccatagttaa actcaatgac cgccactttg ttgatagtga caacgttaac atactgataa
    15481 gacataaaaa ttcctttaat caaaatattg cgtctggata aattcactga gctgccgcag
    15541 gccggtgcgc ggtgagagcg tattgttttt gaccgctaaa agcgtctggc ggtaatagcg
    15601 atcgaaatct tcattcgcga acaggtgatt cagtacttct tcttcggtct gcttacgcag
    15661 ccattccacc gattgttgtt gccgcacttg ttgtaaacga ccactggcag ttagcgcggt
    15721 tttgaagtcg atgatggcgt gccagatctc atcgattcca cgtttttcca gtgcgctaca
    15781 agtcagaacc cgtggctgcc attcgtcgta tttacgtcgc agaatatgca gggcactctc
    15841 gtacatatgc cgggcaatgg cgacattggt atggttatcg ccatcgtctt tgttgataac
    15901 gatcagatca gccacttcca tcagcccttt tttaatgccc tgcagatcat cgccgccacc
    15961 ggcaatttgc aacgagataa aacagtccac catgcgggcg acttctgttt ccgactgccc
    16021 gacgccaacc gtttcgacaa tcactacgtc ataacccgct gcttcgcata acagcattaa
    16081 ttcccgcgct cgctgactgg caccgcccag atgaccggag gatggtaccg ggcgaataaa
    16141 cgccgcttcg gcacgcgcca ggtcattcat gcgggtttta tccccgagaa tgctaccgcc
    16201 agtgaccggg ctgctgggat cgaccgcaat aaccgcgacc tttaatccct ctcgaatcaa
    16261 caacatgcca aaggcctcaa gaaaggtact tttccccgcg ccgggggtgc cggtaacgcc
    16321 cagtcgcagg gtgttaccgc agtacggcat aatggcatca agcagctgcg tacttagtgc
    16381 ctgatgacgc gggtgacggc tttccaccag cgtcatggcc tgggcgagtg tggcacgctc
    16441 accctgacgt aagcggcgaa tactttctgc cagcgtggct tcattaatca tgatgctggc
    16501 ttatcagatt cagtacgtcg cgcacactgt cgagcatagg tgtacctgga ccataaatcg
    16561 ccgccacgcc gcgctcttgc aggaaggcgt aatcctgcgg cggaatgacg ccacccgcga
    16621 ccacgcagat atcttcgcgt ccccattttt tcagcgcttc gaccagttcc gggatcagcg
    16681 ttttatgacc ggcagccagt gaggatgcgc ccactacgtg aacgtcgttt tctacggcca
    16741 ggcgggcgat ctcttcaggt gtagagaaca tcgggcttaa atctacgtcg aaaccgagat
    16801 cggaataggc gctggcgatc actttcgcgc cgcgatcgtg tccatcctgg cccatcttag
    16861 cgatcagaat gcgcgggcga cgaccattgt cggcaaggaa ctgctccgtt tgcgcaacaa
    16921 tggcatcgaa ctcggaggcc gatttctcag actgatgata gctttgcgca atcacgccgg
    16981 taacacactg gcttggcacc agataacggt cgaaagcgac ttccagcgca tcggaaattt
    17041 cacccagggt ggcgcgaacg cgagcggcat taacagcggc agccagcagg ttttcgttat
    17101 gctgtgcggc gtgagtcagg gcgttcaacg cggcggttac ggcggcatca tcacgggtgg
    17161 cgcgaatgcg ttccagcgaa gcaatttgct cgttacgcac catcacgttg tcgatctcaa
    17221 gtacatcggt ttcgtcttcg tgatccagtt tgtacttgtt gacaccaacg atgacacgct
    17281 tgccctggtc gatcagcgac tgttcgcgcg ctgaggcctc ttcgatcatt cgttttggca
    17341 gacctgcttc gatcgctttc gccatgccac cggcttcgtc gatctgttgg ataatagctc
    17401 tggcttgttt gacgatttga tcggtcagcg actcaatgta ataggatccg gccagtggat
    17461 cgacggtgcg gcagagttct gattcttcct ggatgatgat ctgggtgttg cgggcaatgc
    17521 gtgctgagaa atcggtaggc aaaccaagcg cttcgtcaaa ggcgttggta tgcagtgact
    17581 gagtaccgcc cagcgtcgca gccagcgctt caatggtggt gcggataacg ttgttatacg
    17641 gatcctgttc agtcaggctc cagcctgagg tctggcagtg ggtacgcagc gccagtgatt
    17701 tcgggtcctg tgcgccaaat ccactgaccg cttcgctcca taaataacgt gccgcacgca
    17761 acatggcgac gttcataaac agatccatgc cgatgccgaa gaagaacgac aggcgaggag
    17821 cgaagtcatc aattttcagt ccggcagaga ttgctgcttt gatgtactca atcccatcag
    17881 cgagcgtaaa tgctacctgc tgcacgcagt tggcacccgc ttcacccatg tggtaaccgc
    17941 tgatactgat ggtattaaat cgcggcatgt tgccggaaca ccaggcgatg atgtcggcga
    18001 taatgcgcat tgacggtttt ggtgggtaaa tataggtgtt gcggcagagg tactctttga
    18061 gaatatcgtt ttgaatggtg ccggtcagtt tatcaggtgt aacaccttgc tcttctgcgg
    18121 cgacgatata aaacgccagt actggtagca ctgcgccatt catggtcatc gaaaccgaca
    18181 ttttatccag cgggatctgg tcgaacagga ctttcatatc ttccacggtg tcgatagcga
    18241 cgcccgcttt gccgacgtcg cccgccacgc gcgggttatc ggagtcgtag ccacggtggg
    18301 tggcaaggtc aaacgcaacg gaaagacctt tttgcccggc ggccaggtta cggcgataaa
    18361 aagcgttgga ctcttttgct gttgaaaaac cagcatactg acggatggtc cacggttggg
    18421 cggtatacat agtggcacgc gggccacgaa cgtagggcgg caaaccagga agggtacctg
    18481 tcacctccag attatcgaga tcggcttcgg tatacagcgg cttgatggcg atcccttccg
    18541 cggtttgatg aaccagcgag tcgacagttt tctcccgacg gctcaattcc ttgttggcaa
    18601 gctgttgcca ctcctgcacg ttagacatta tttgctccgt aaaacgcaat caaaatgagg
    18661 gtatttgcgc ctacggtgag ctttttacgt gggtttgtcg ccagcaaaaa aaatgcattc
    18721 agacgtcgtt ttttgccgcc tgagatttgt ctattttgtg atgtgattca aggaatacaa
    18781 aaagaagtga ggacaacgcc tgatatgtgc tatcaggcat tatttgatgg attaatcctg
    18841 acgaaggact ttgtgaccat aatcgagtaa cgcatcagtg actttacgca tcatgcggct
    18901 ttcaggagca aagcggtgcc agtagagcat ccgtcgttga aatagcccag gcgttaagtc
    18961 aatcagttca ccgctggcca gctctttctc gatttgcagg tgcgggatca tacagcaggt
    19021 ggtgccctgg cgagcaagtt gtacgaacgc ttctgaagaa ttaacgatat ggcagggtac
    19081 gctgcctgga ggcagatcga agttttgctg caaaaaggcc tggtgcatat cgtcaagatg
    19141 gtcaaacgcg accactggcg ctttcagtaa tgccgaacgc gttacgccgt tagggaaata
    19201 tttttcggca aagggttttg agctgacgaa cagatagtcg agcgcaccaa gtttatcgac
    19261 aagacaactc ggcagcgcct gatgttgaat actcaccgcg ccgaccactt cgccgcggcg
    19321 cagacgttcc tgagtgcggg tttcatcttc tacctgcaag ttgaggcgga taggcgaatc
    19381 agccaacaca ggagccagtg caggaagcaa ccacgtcgcc agactgtcgg cgttgaccgc
    19441 cagtgaaagc agcagcggag tcgaaccggt ttgttcatcg cccagccact cttcttccag
    19501 caactccacc tggcgcagca gtgccagcag tttttgccct tgttccgtcg ggcgcggcgg
    19561 tacggtacgc accaacagcg gctgcccgaa catattttcc agttgcttaa tgcgctgtga
    19621 gacggctgat tgtgtaatgc acagcttttg tgccgcgcgc tcaaatcctc gttcacgtat
    19681 caccgcatcc agtgcctgta atgttctgta gtccgggcgt ttcattgctg ttgtctgctc
    19741 ctggtaaatg ctttttcctg cactatgaca caattttgtg tcaggttgca tataccgaag
    19801 ccgtcggcgg gtaacatttg tatccgtggt taaaagcgca gcttaaaaga tcagggattt
    19861 gcgctaatgc atttcgctca gcgagttcgc gccctggttg tgttgaatgg cgtggcgtta
    19921 ttgcctcaat ttgcctgtaa acaggggctt gcgaacggtg aactggtgcg cctgtttgca
    19981 ccgtggagcg gcatacccag accgttgtat gctttatttg cggggcgaaa ggggatgcct
    20041 gccattgcgc gatattttat ggatgagtta accacgcggc ttgccaacgg ggtctgaatc
    20101 gctttttttg tatataatgc gtgtgaaatt tcataccaca ggcgaaacga tcatgacgca
    20161 ggatgaattg aaaaaagcag taggatgggc ggcacttcag tatgttcagc ccggcaccat
    20221 tgttggtgta ggtacaggtt ccaccgccgc acactttatt gacgcgctcg gtacaatgaa
    20281 aggccagatt gaaggggccg tttccagttc agatgcttcc actgaaaaac tgaaaagcct
    20341 cggcattcac gtttttgatc tcaacgaagt cgacagcctt ggcatctacg ttgatggcgc
    20401 agatgaaatc aacggccaca tgcaaatgat caaaggcggc ggcgcggcgc tgacccgtga
    20461 aaaaatcatt gcttcggttg cagaaaaatt tatctgtatt gcagacgctt ccaagcaggt
    20521 tgatattctg ggtaaattcc cgctgccagt agaagttatc ccgatggcac gtagtgcagt
    20581 ggcgcgtcag ctggtgaaac tgggcggtcg tccggaatac cgtcagggcg tggtgaccga
    20641 taatggcaac gtgatcctcg acgtccacgg catggaaatc cttgacccga tagcgatgga
    20701 aaacgccata aatgcgattc ctggcgtggt gactgttggc ttgtttgcta accgtggcgc
    20761 ggacgttgcg ctgattggca cacctgacgg tgtcaaaacc attgtgaaat gatctgacgg
    20821 gggaacctcc cccgttaaaa aaattctctt cattaaattt ggtgacatgt gtcacgcttt
    20881 taccaggcaa ttgtcgattg ctctaaataa atcctctaaa ccagcatatt catccaagaa
    20941 ttacctttgc gtgatatttc ctcaacatcg cgacgcaaac gttcatattg ccgcaatatt
    21001 attttttgat atgttgaaag gcggatgcaa atccgcacac aacatttcaa aagacaggat
    21061 tgggtaaatg gcaaaggtat cgctggagaa agacaagatt aagtttctgc tggtagaagg
    21121 cgtgcaccaa aaggcgctgg aaagccttcg tgcagctggt tacaccaaca tcgaatttca
    21181 caaaggcgcg ctggatgatg aacaattaaa agaatccatc cgcgatgccc acttcatcgg
    21241 cctgcgatcc cgtacccatc tgactgaaga cgtgatcaac gccgcagaaa aactggtcgc
    21301 tattggctgt ttctgtatcg gaacaaacca ggttgatctg gatgcggcgg caaagcgcgg
    21361 gatcccggta tttaacgcac cgttctcaaa tacgcgctct gttgcggagc tggtgattgg
    21421 cgaactgctg ctgctattgc gcggcgtgcc ggaagccaat gctaaagcgc accgtggcgt
    21481 gtggaacaaa ctggcggcgg gttcttttga agcgcgcggc aaaaagctgg gtatcatcgg
    21541 ctacggtcat attggtacgc aattgggcat tctggctgaa tcgctgggaa tgtatgttta
    21601 cttttatgat attgaaaata aactgccgct gggcaacgcc actcaggtac agcatctttc
    21661 tgacctgctg aatatgagcg atgtggtgag tctgcatgta ccagagaatc cgtccaccaa
    21721 aaatatgatg ggcgcgaaag aaatttcact aatgaagccc ggctcgctgc tgattaatgc
    21781 ttcgcgcggt actgtggtgg atattccggc gctgtgtgat gcgctggcga gcaaacatct
    21841 ggcgggggcg gcaatcgacg tattcccgac ggaaccggcg accaatagcg atccatttac
    21901 ctctccgctg tgtgaattcg acaacgtcct tctgacgcca cacattggcg gttcgactca
    21961 ggaagcgcag gagaatatcg gcctggaagt tgcgggtaaa ttgatcaagt attctgacaa
    22021 tggctcaacg ctctctgcgg tgaacttccc ggaagtctcg ctgccactgc acggtgggcg
    22081 tcgtctgatg cacatccacg aaaaccgtcc gggcgtgcta actgcgctga acaaaatctt
    22141 cgccgagcag ggcgtcaaca tcgccgcgca atatctgcaa acttccgccc agatgggtta
    22201 tgtggttatt gatattgaag ccgacgaaga cgttgccgaa aaagcgctgc aggcaatgaa
    22261 agctattccg ggtaccattc gcgcccgtct gctgtactaa ttccccttct ctgaaaatca
    22321 acgggcaggt cactgacttg cccgtttttt tatcccttct ccacaccgtt gccgcgttgc
    22381 gcattctact ttgcgagtct gctccagaaa ataaatcaaa gtcagatcaa tgcgttgtgt
    22441 ttatttaatg gggatttacg atggcagggc agcatgggct gtaacggtaa agccctcacc
    22501 gaagcgaggg cttgaaggag aagggttatg atgcgacttg tcatcatact gattgtactg
    22561 ttactcataa gtttcagcgc ttattaacag tcagtctcag gggaggagca atcctccctt
    22621 acccttactc actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcgggtgt
    22681 atcgccctta ccactcccag actttcgacg gtgtaaccac cgcaggaaga gggatatccc
    22741 actcttcaac ggggagtttt tccaccaact gacaatcatg cgcataaccc accggttgcg
    22801 ttttatagtg ctgccagttt tgtaaggtcc gatcataaaa accaccgccc attcccaggc
    22861 gctgaccgta ctcatcaaag gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg
    22921 gtagcacgtc acgcacatcc aattttggct catggatctt caacctgttc atcaccagtt
    22981 cgctttgcgg atggtaattc aggaacagca aattaccggc actaaaggga tgcaaaactg
    23041 gaagatatac gcgcttaccg gcgcgccaga gttgttctat gagtggctgg gtgtcgagtt
    23101 cgccatcaaa agagaggaat acagcgaccg tatgtgccat caccaccggg ggataagtca
    23161 tcatccgggt agcggcttgt tgacccattt cctgctgttg ttccggcgtt aacgcacgac
    23221 gacgttgccg aatcattttg cggatttctt gtcgggataa tgtcagtggg agttctggta
    23281 gttgcgtcat ggctggtgcc agataagaag ggaatctccg agatgccgcc gcaggctgta
    23341 acccttgaac ccttggttca aggtgaatgt gtcgtcgcag ttttaaggct tctcggacgg
    23401 accgagcatg ctcaccaacc gcggagcgcc acattcttgt ggtatgaaat atcggctcag
    23461 gggactggcc cgcttgcgaa catctcagag aaattttgtc ttcacggtta ctctaccaca
    23521 gtaaaccgaa aagtgtcatt caaagttttg gttagttttt tcggtgatgc gaccttgttc
    23581 aagtaacgct tgttctatgg tctgctgcag catccgaata cgctgttcca tacttgccgc
    23641 gtagtcacga gtctttgctt tttcttgcgc taactcatag ctgatattca atgcggcaat
    23701 gaagaccaac tgttcagtat ttgtgactct agtgcgttct ttcagatctt gcaaccgttg
    23761 gttcagatcg tccgctgcct gattcaacgc atccctttgg tcaggcgggc agttcacacg
    23821 cagtgaacgg ccaaaaattt ggatatcgac gggttgtgca gacatgccac cttcctgctg
    23881 attgactgcg ctgccttcgt ctttagaccc atggtctgcg aaggggcgac actatagcta
    23941 ccctgatgag aagagacaag cccttttctg gtccaccagg gcccaaagtg gtagcatatc
    24001 atgaatattc ctccctttga cgacgaatgc ttatgtctat acagaacgaa atgcctggtt
    24061 acaacgaaat gaaccagtat ctgaaccaac aagggacggg tctgacccca gctgagatgc
    24121 atggtttaat cagcgggatg atatgtggcg gtaacgatga cagctcatgg ctaccgctac
    24181 ttcacgacct gacgaacgaa ggcatggctt tcggtcatga gctggcacag gcactgcgta
    24241 aaatgcactc tgccaccagc gatgccctgc aggatgacgg cttccttttt cagctttatc
    24301 tgcctgatgg cgatgatgtc agcgttttcg atcgggctga tgcattggca ggttgggtca
    24361 atcacttcct gcttggtctt ggcgttacgc aaccgaagct ggataaagtg accggcgaaa
    24421 ccggtgaagc tatcgacgat ctgcgtaaca ttgcgcaact gggttacgac gaagacgaag
    24481 atcaggaaga gcttgaaatg tcgcttgaag agatcatcga atacgttcgt gttgccgcgc
    24541 tgttatgcca cgacaccttt actcatccgc aaccgaccgc gccagaagta caaaaaccga
    24601 ctctacacta aaaacaaaaa acgtaaggag agtgttatga gtgagatatc ccggcaagag
    24661 tttcagcgtc gccgtcaggc cctggtggag caaatgcaac ccggcagcgc cgcgctgatt
    24721 tttgctgcac cagaagtaac acgtagcgcc gacagcgaat acccctatcg tcagaacagt
    24781 gacttctggt acttcaccgg ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc
    24841 gatgacactc ataaccacag cgttctgttt aaccgcgttc gcgacctgac ggcggagatc
    24901 tggtttggcc gtcgcttagg ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca
    24961 ctggcattca gcgaaatcaa tcagcaactt tatcaactac ttaacggcct ggatgtggtt
    25021 taccatgccc agggcgaata tgcatatgct gatgtaatcg tgaacagtgc gctggaaaaa
    25081 ctgcgtaaag gttcgcggca aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct
    25141 gttgttcatg aaatgcgcct gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg
    25201 ggagaaatca ccgccatggc acatacacgg gcgatggaaa aatgccgtcc gggaatgttc
    25261 gagtaccatc tggaaggcga aattcaccac gaatttaacc gccacggtgc gcgctatccg
    25321 tcctataaca ccattgtcgg cagcggtgaa aacggctgca ttctgcacta caccgaaaac
    25381 gagtgtgaaa tgcgcgacgg cgacctggtg ttgattgacg cgggttgtga atacaaaggt
    25441 tacgctggcg atattacccg caccttcccg gtcaacggca aattcaccca ggcccagcgt
    25501 gaaatctacg acattgtgct ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga
    25561 acttccattc tggaagtcac tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa
    25621 ctcggcatcc tgaaaggtga tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc
    25681 tttatgcatg gccttagcca ctggttagga ctggatgtcc atgacgtggg tgtttatggt
    25741 caggatcgct cgcgcattct ggaaccgggc atggtactga ccgtagagcc tgggctgtat
    25801 attgcgccgg atgcagaagt gccagaacaa tatcgcggta tcggcattcg tattgaagac
    25861 gacattgtga ttaccgaaac cggtaacgaa aacctcaccg ccagcgtggt gaaaaagccg
    25921 gaagaaatcg aagcgttgat ggttgctgcg agaaagcaat gagcgtaatc atcgttggtg
    25981 gcggcatggc gggcgcgacg ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc
    26041 cggtacattt gattgaagcg actgcgccag agtcacatgc tcatccgggc tttgatggac
    26101 gagcgatagc gctggcggcg ggtacctgtc agcaactggc gcgcatcggc gtctggcaat
    26161 ctctggcgga ttgcgcaact gccatcacca ccgtgcatgt cagcgatcgt ggtcacgctg
    26221 gatttgtcac cctcgccgca gaagattacc aactggcggc gctgggacag gttgtcgaat
    26281 tgcacaatgt cgggcaacgg ctgtttgcat tgctgcgtaa agcacctggc gtaacgctgc
    26341 attgccctga tcgcgtggct aacgttgccc gtactcagag tcacgttgaa gtgacgctgg
    26401 agagtggcga gacgctgacg ggccgcgtgc tggtagcagc tgatggcacc cattcagcgt
    26461 tagccaccgc gtgcggcgtt gactggcagc aggagcctta cgaacaactg gccgtgattg
    26521 ccaacgttgc tacttccgtt gcgcatgaag ggcgcgcttt tgaacgcttt acgcaacatg
    26581 gcccgctggc gatgttgccg atgtctgacg gacgctgttc gctggtctgg tgtcatccac
    26641 tggaacggcg cgaagaggtg ttgtcgtgga gtgacgagaa gttttgccgt gaactccagt
    26701 cggcctttgg ctggcgactt gggaaaatta cccacgctgg taaacgcagt gcttatccgc
    26761 tggcgttaac ccacgccgcc agatctatta cccatcgtac cgtgctggtg ggcaatgcgg
    26821 cgcaaactct gcacccgatt gccgggcaag ggtttaacct cggtatgcga gatgtgatga
    26881 gtcttgcgga aaccctgact caggcgcagg agcgcggaga agacatgggg gattacggcg
    26941 tattgtgccg ttatcagcag cgtcgacaga gcgatcgcga agcaaccatt ggcgtcacgg
    27001 acagccttgt acatcttttt gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg
    27061 ggctgatgac gatggaatta ttcatcccgg cacgcgatgt gctggcgcag cgcaccctcg
    27121 gttgggtggc gcgttgaagg cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc
    27181 attgttggcg gcggcatggt ggggctggcg gttgcctgtg gcttacaggg gagcggctta
    27241 cgcgttgccg tactggagca gcgcgtacag gaacctctgg cggcgaatgc accaccacaa
    27301 ctgcgcgttt cggctatcaa tgccgccagc gaaaaattac tcacccgtct tggcgtctgg
    27361 caggacattc tctctcgtag ggccagctgt tatcacggta tggaagtgtg ggacaaagac
    27421 agctttggtc acatttcgtt tgacgatcaa agcatgggct atagccatct tgggcatatc
    27481 gttgaaaatt cagtgattca ctacgcgctg tggaacaaag cgcatcagtc gtcagatatc
    27541 actctgttag cccccgcaga attacagcag gtcgcctggg gagaaaatga aaccttcctg
    27601 acgctgaaag atggcagcat gttaacggcg cgtctggtga ttggcgcgga cggcgctaat
    27661 tcctggttgc gcaacaaagc cgatattccg ctgacttttt gggattatca gcatcacgcg
    27721 ctggtagcga ccattcgcac ggaagaaccg catgatgcgg tggcgcggca ggttttccat
    27781 ggcgaaggca ttctggcctt tttaccgctt agcgatccgc atctttgctc gattgtctgg
    27841 tcactgtcgc cagaggaagc gcagcggatg cagcaggcaa gtgaagacga atttaatcgc
    27901 gcgttaaata tcgcttttga taatcgcctg ggcttatgca aggttgagag cgcgcgtcag
    27961 gtgttcccac tgacggggcg ttatgcgcgc cagtttgcct cgcaccgtct ggcgctggtg
    28021 ggcgacgccg cacataccat tcacccgctg gcggggcagg gggtaaatct cggctttatg
    28081 gatgctgcag agctgattgc cgaactgaaa cggttgcatc gtcaggggaa agacatcggg
    28141 cagtacattt atctgcgtcg ctatgagcgt agccgcaagc acagtgcggc gttgatgctg
    28201 gctggtatgc agggattccg cgatctgttt tccggtacca atccggcgaa aaaactgctg
    28261 cgtgatattg gtttgaaact ggccgacacg cttcctggcg ttaagccgca acttatccgc
    28321 caggcaatgg gattaaacga tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt
    28381 ttatttgata cccatcacac tttcatctcc cggttttttc gccgggagat tttcctcatt
    28441 tgaaataaac taatttcacc tccgttttcg cattatattt tctaatgcca ttattttttg
    28501 atttagtgtt ttttgacatt tttttagctc ttaatattgt cttattcaaa ttgactttct
    28561 catcacatca tctttgtata gaaactggtg tattttttgg ttttttattc tgtcgcgatt
    28621 tttgcatttt ttaaccataa gctaatgtga tgatcaattt taccttatgg ttaacagtct
    28681 gtttcggtgg taagttcagg caaaagagaa cgattgcgtt ggggaccggg agtggctccg
    28741 atgctgggtt tcgtggtgat aatttcacca tgaaaaagtt gtcagccccg cttattcaat
    28801 gaggacaaga tggcacaaca gactcctttg tacgaacaac acacgctttg cggcgctcgc
    28861 atggtggatt tccacggctg gatgatgccg ctgcattacg gttcgcaaat cgacgaacat
    28921 catgcggtac gtaccgatgc cggaatgttt gatgtgtcac atatgaccat cgtcgatctt
    28981 cgcggcagcc gcacccggga gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc
    29041 accaaaagcg gcaaagccct ttactcgggg atgttgaatg cctctggcgg tgtgatagat
    29101 gacctcatcg tctactactt tactgaagat ttcttccgcc tcgttgttaa ctccgccacc
    29161 cgcgaaaaag acctctcctg gattacccaa cacgctgaac ctttcggcat cgaaattacc
    29221 gttcgtgatg acctttccat gattgccgtg caagggccga atgcgcaggc aaaagctgcc
    29281 acactgttta atgacgccca gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg
    29341 caggcgggcg atctgtttat tgccaccact ggttataccg gtgaagcggg ctatgaaatt
    29401 gcgctgccca atgaaaaagc ggccgatttc tggcgtgcgc tggtggaagc gggtgttaag
    29461 ccatgtggct tgggcgcgcg tgacacgctg cgtctggaag cgggcatgaa tctttatggt
    29521 caggagatgg acgaaaccat ctctccttta gccgccaaca tgggctggac catcgcctgg
    29581 gaaccggcag atcgtgactt tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt
    29641 acagaaaaac tggttggtct ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg
    29701 gtacgcttta ccgatgcgca gggcaaccag catgaaggca ttatcaccag cggtactttc
    29761 tccccgacgc tgggttacag cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg
    29821 gcgattgtgc aaattcgcaa ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg
    29881 cgtaacggca aagccgtcgc gtgatttact tttttggaga ttgattgatg agcaacgtac
    29941 cagcagaact gaaatacagc aaagaacacg aatggctgcg taaagaagcc gacggcactt
    30001 acaccgttgg tattaccgaa catgctcagg agctgttagg cgatatggtg tttgttgacc
    30061 tgccggaagt gggcgcaacg gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa
    30121 aagcggcgtc agacatttat gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac
    30181 tgagcgattc cccggaactg gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa
    30241 tcaaagccag cgatgaaagc gaactggaat cactgctgga tgcgaccgca tacgaagcat
    30301 tgttagaaga cgagtaacgg ctttattcct cttctgcggg agaggatcag ggtgaggaaa
    30361 atttatgcct caccctcact ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt
    30421 caggaaccat cgctcatgac acagacgtta agccagcttg aaaacagcgg cgcttttatt
    30481 gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca
    30541 caatcgttaa acgcgctgac cggccagatt gtgccgaaag atattcaact tgcgacacca
    30601 ccgcaggttg gcgcaccggc gaccgaatac gccgcactgg cagaactcaa ggctattgcc
    30661 agtcgcaata aacgcttcac gtcttacatc ggcatgggtt acaccgccgt gcagctaccg
    30721 ccggttatcc tgcgtaacat gctggaaaat ccgggctggt ataccgcgta cactccgtat
    30781 caacctgaag tctcccaggg ccgccttgaa gcactgctca acttccagca ggtaacgctg
    30841 gatttgactg gactggatat ggcctctgct tctcttctgg acgaggccac cgctgccgcc
    30901 gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg
    30961 gcttccgatg tgcatccgca aacgctggat gtggtccgta ctcgtgccga aacctttggt
    31021 tttgaagtga ttgtcgatga cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg
    31081 ctgttacagc aggtaggcac taccggtgaa attcacgact acactgcgct tattagcgaa
    31141 ctgaaatcac gcaaaattgt ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta
    31201 actgcgccgg gtaaacaggg cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg
    31261 ccgatgggct acggtggccc acacgcggca ttctttgcgg cgaaagatga atacaaacgc
    31321 tcaatgccgg gccgtattat cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc
    31381 atggcgatgc agactcgcga gcaacatatc cgccgtgaga aagcgaactc caacatttgt
    31441 acttcccagg tactgctggc aaacatcgcc agcctgtatg ccgtttatca cggcccggtt
    31501 ggcctgaaac gtatcgctaa ccgcattcac cgtctgaccg atatcctggc ggcgggcctg
    31561 caacaaaaag gtctgaaact gcgccatgcg cactatttcg acaccttgtg tgtggaagtg
    31621 gccgacaaag cgggcgtact gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat
    31681 attctgaacg cggttgggat cacccttgat gaaacaacca cgcgtgaaaa cgtaatgcag
    31741 cttttcaacg tgctgctggg cgataaccac ggcctggaca tcgacacgct ggacaaagac
    31801 gtggctcacg acagccgctc tatccagcct gcgatgctgc gcgacgacga aatcctcacc
    31861 catccggtgt ttaatcgcta ccacagcgaa accgaaatga tgcgctatat gcactcgctg
    31921 gagcgtaaag atctggcgct gaatcaggcg atgatcccgc tgggttcctg caccatgaaa
    31981 ctgaacgccg ccgccgagat gatcccaatc acctggccgg aatttgccga actgcacccg
    32041 ttctgcccgc cggagcaggc cgaaggttat cagcagatga ttgcgcagct ggctgactgg
    32101 ctggtgaaac tgaccggtta cgacgccgtt tgtatgcagc cgaactctgg cgcacagggc
    32161 gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa gccgcaacga agggcatcgc
    32221 gatatctgcc tgatcccggc ttctgcgcac ggaactaacc ccgcttctgc acatatggca
    32281 ggaatgcagg tggtggttgt ggcgtgtgat aaaaacggca acatcgatct gactgatctg
    32341 cgcgcgaaag cggaacaggc gggcgataac ctctcctgta tcatggtgac ttatccttct
    32401 acccacggcg tgtatgaaga aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc
    32461 ggtcaggttt accttgatgg cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc
    32521 tttattggtg cggacgtttc acaccttaac ctacataaaa ctttctgcat tccgcacggc
    32581 ggtggtggtc cgggtatggg accgatcggc gtgaaagcgc atttggcacc gtttgtaccg
    32641 ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc agggcgcggt ttctgcggca
    32701 ccgttcggta gcgcctctat cctgccaatc agctggatgt acatccgcat gatgggcgca
    32761 gaagggctga aaaaagcaag ccaggtggca atcctcaacg ccaactatat tgccagccgc
    32821 ctgcaggatg ccttcccggt gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt
    32881 attctcgata ttcgcccgct gaaagaagaa accggcatca gcgagctgga tattgccaag
    32941 cgcctgatcg actacggttt ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg
    33001 atggttgaac cgactgaatc tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg
    33061 ctggctatcc gcgcagaaat tgaccaggtg aaagccggtg tctggccgct ggaagataac
    33121 ccgctggtga acgcgccgca cattcagagc gaactggtcg ccgagtgggc gcatccgtac
    33181 agccgtgaag ttgcggtatt cccggcaggt gtggcagaca aatactggcc gacagtgaaa
    33241 cgtctggatg atgtttacgg cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa
    33301 taccagtaat tcactgattc gactattttc taaaggcgct tcggcgcctt tttagtcaga
    33361 tgacaaagta caaaagtgct cagacagtcc cctcgcccct ttggggagag ggttagggtg
    33421 aggggaacag gccggcactg gcgcgaatat ttaccctcac cccggccctc tccctgaaag
    33481 ggcgaggggg aaaagcgtgc caacattgaa gattgagcca gtttgttagc aatctcaaag
    33541 atacgtcaac gaattaattt ttctcggaaa aacaaatggc tatagcactt gtgactggtg
    33601 gcagtcgcgg catcgggcgg gcaactgcat tactgttggc gcaagaaggg tatacggtgg
    33661 cggttaatta tcagcaaaac ctccacgcgg cgcaggaagt gatgaactta ataacgcaag
    33721 ccggtggcaa agcattcgtg ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga
    33781 tgtttacagc aatcgatcag cacgatgaac cgctagcagc gctggtcaat aatgccggga
    33841 tcttgtttac ccagtgcacc gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt
    33901 ccaccaacgt gacgggatat tttctctgct gccgcgaggc ggtaaaacgc atggcgctta
    33961 aaaatggtgg cagtggcggc gctatcgtca atgtctcttc ggtggcctca cggttgggtt
    34021 cgccagggga atatgttgat tacgcggcat cgaaaggggc gattgatacg ttaaccaccg
    34081 gactatcgct ggaagtcgcc gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta
    34141 tttataccga aatgcacgcc agcggcggcg agcctggacg cgtcgatcgc gttaagtcga
    34201 acatccccat gcagcgtggt ggacaggcag aagaggtcgc gcaggccatt gtctggctac
    34261 taagtgataa agcctcttac gtcacgggaa gttttatcga tttggcgggc gggaaataaa
    34321 acagggaagt tgtctgaccg gatgcaacaa gtattgcatc cggtacttca tcgacttaaa
    34381 gcttctcgcc gttgctggca atcacctctt tgtaccagtt aaagctcttc ttacgtgaac
    34441 gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg tagcgtttgc
    34501 tgtactgccc ggtggtgaac gacacgcagt cgatgcagcc ccacggtgtg tagcccatca
    34561 gatccacgcc atcgtaagtc accgcttttt tcatctcttc gatatgggcg cgcaggtagt
    34621 caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa gcgccaaaac
    34681 cgttttcgac aataaacagc ggcctctgat aacgctcata cagttcgcaa agtgcatagc
    34741 gcagacctac tggatcaatc tgccagcccc agtcagatgc tttaacatac gggtttggta
    34801 cgctgccttc aaaaccagag atcgcatcgc cggtgccgcc ttcggccttc actgcattgg
    34861 tcatgtaata gctgaaacca agataatcgc aggtgccttc acgcagcaca tccagatcgc
    34921 cgtcttccat tttgatgtta aatccgcgac gctcccactc gttcaacaca taggacgggt
    34981 aatagccgcg tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga
    35041 acatcacatc gtccgggtta caggagtaag gatagagcgg caccatcgcc agcatacagc
    35101 cgactttcat ctccgggtta atgcgacgcg cagctttcac cgccagggcg ctggcgacaa
    35161 actggtgatg cagcacctga tacatcgtct cttccgggtt ttcatgctcg gtatacacca
    35221 cgccggagca gcagtaaccg aacagcggtg cacgccagtt acgctggttg ttaatttcgt
    35281 tgaaggtcat ccagtatttg actttgtgct tatagcgttc aaataccact tccgcgaaac
    35341 gtacaaagaa atcaaccact ttacggttgg tccagctacc gtattgctgc accagatgca
    35401 gcggcatttc aaagtgggag agggtgatca ccggttcgat gttgtatttc agcagttcat
    35461 cgaacatatc atcgtagaac ttcagccctt cttcgtttgg ctgagcttca tcgccttttg
    35521 gaaaaatgcg cgtccaggca atggatgtac gaaaacattt gaagcccatt tcggcaaata
    35581 gcttgatgtc ttccttatag tgaccataaa aatcaacggc ttcatggttt ggatagtatt
    35641 ttcctggcaa gacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca ccggtcagaa
    35701 cgtcacaaat gctcggccct tttccgcctt tgttccagcc gccttcgacc tgatgagcgg
    35761 caactgcgcc gccccataag aaatctttcg gtaaggtgag ttttttcact atcataggct
    35821 ccagatgagt caattcttac aggaaatagt accatgcagc caaacgacat cacttttttt
    35881 caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg cgacgagtct
    35941 gaatcgcact tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga tgacggttat
    36001 ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacgct gactgaaaaa
    36061 catgcagagc aggaaaatat gaccctgact gaactgaaaa aagtcattgc tgacatctat
    36121 cccggtcaga cacaatttta tgtgattgaa tttaaatgtc tttaaattga actcacgcct
    36181 gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc aaatcagaat
    36241 ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag aggtatacat
    36301 gtgcgtgtac gtttctccgg agtaagttat ggttcagaag cccctcatta agcagggata
    36361 ttcactggca gaggaaatag ccaacagcgt cagtcacggc attgggttgg tgtttggtat
    36421 cgttgggctg gtgttgctac tggttcaggc ggtggatctt aatgccagcg ccacagcgat
    36481 aaccagctac agcctctatg gcggcagtat gatcctgctg ttcctcgctt cgacgctcta
    36541 tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc attgcgctat
    36601 ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg attctccgtt
    36661 agcgcgcggg ttgatgattg ttatctggag cctggcattg ctgggtattc tgtttaaact
    36721 gaccatcgcg caccgattca aaattttatc tctggtgacc tatctggcga tgggctggct
    36781 gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg ttaccttact
    36841 ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca aacgcattcc
    36901 atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc actttctggc
    36961 gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg
    37021 ccagcgtcgc atcaggcatc ggagcacaac tgccggattc ggcgtgaacg ccttatccga
    37081 tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga taagacgcgc
    37141 cagcgtcgca tcaggcgata aatgcctgat gcgataaaga gatttactct tcgagcgaat
    37201 acggcagcgg ctcgatatgc aatgtattcg catcgtcgcg tacgcggaag atgctatccg
    37261 gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc agttttaccg
    37321 cagccagtac cgtaccggta cgacgccagt tctcgcccat tttcagctct aagtcttcac
    37381 cagcttccgg cagtcggctg gcgctacctg ccagcaacca gagcgcacgt ttattggcac
    37441 cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc ttaaagctga
    37501 taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat
    37561 caatcaccgg gaaacccgct tcaatgttta atgccagcca ctgttggcta ttgttcagtt
    37621 ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg gttacgatca
    37681 ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct ttgactacct
    37741 gtttttcttt cgaaggcagt tcgctaaaga gatttgccag cgcggcgcgc gcctgaaaac
    37801 cggcaacacc aagcagcaca cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg
    37861 catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca atccatgcaa
    37921 agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg gcgtcgcaat
    37981 gggcggcgag caggtgctga tcttctgcca tctggctgac atctgctgtc acctgaccct
    38041 gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca
    38101 tcagcgtcag cggtaaacgg gcagaagccg taggctgacg gggaggaaaa ggtgtaaaag
    38161 ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa aagagctatg
    38221 aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac ttccaaaaaa
    38281 atgaagccat tgaattgttg tgaattaatt tcgcgatcgc gatggtgttt actgatattc
    38341 ccgtaaaaga aggcgaaaaa cacgttacaa tagccgggta ctggattttc gtgagacagg
    38401 aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg tggtatgcgc
    38461 gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt aagcgatgac
    38521 gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt taactggctg
    38581 atgaatcacg gtaaaccggc cgatgcagaa ctggaaatga tggtccgact catccagaca
    38641 cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg
    38701 gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccact
    38761 cagttacacc ccgttatgga tggtgttact ttcgctggtg gtgtttgatt gcgttcgcag
    38821 ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg ggcgtttgcg
    38881 ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat
    38941 gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag ccgacagcat
    39001 ggacgaagct gaatggcggg atttacggcg gattttgttg caacaagaga cgcaaagata
    39061 aagatgacgg caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca
    39121 tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg tcatactggt
    39181 tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc
    39241 tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct ttggtcgaga
    39301 gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc
    39361 caagcccata cagcgcaaca attttacctt caaggttcag gtcgtcgagc tgatcccaga
    39421 cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca
    39481 catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt
    39541 ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac
    39601 cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgcggct
    39661 agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc
    39721 ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg atgctctgtg
    39781 gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat
    39841 ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg acgatttgca
    39901 ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag cgcgtttgct
    39961 gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc
    40021 cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc
    40081 gcaggtcgaa cgtttattac aggcaccatt aatcgatcag ccactggagc tacgcgataa
    40141 agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg tcggactgac
    40201 aatgagtgat atcagcctgc gtcagggcgt ggtacgggtc attggtaaag gcaacaaaga
    40261 gcgtctggtg ccgttaggtg aagaggcggt ttactggctg gaaacctatc tggaacatgg
    40321 gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca
    40381 gcagatgacg cgacagacct tctggcaccg tattaaacat tatgctgtgc tggcgggtat
    40441 cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc atttattaaa
    40501 tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc tctccaccac
    40561 gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc
    40621 gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta tgttgtttac
    40681 tttgttagcg gcgttttcag gctttgctca ggctgatgac gcggcaattc aacaaacgtt
    40741 agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cctgtagctg gcatgaagac
    40801 agttctgact aacagcggcg tgttgtacat caccgatgat ggtaaacata tcattcaggg
    40861 gccaatgtat gacgttagtg gcacggctcc ggtcaatgtc accaataaga tgctgttaaa
    40921 gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg aaaaacacgt
    40981 catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg agcaaatggc
    41041 agactacaac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga
    41101 cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt
    41161 tgatgatgtg atggcaggta aaagcgtcgc accagccagt tgcgacgtgg atattgccga
    41221 ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg tgctgagcaa
    41281 tggcacactt gttccgggtt accagcagcc gaaagagatg aaagaattcc tcgacgaaca
    41341 ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact tcgtcgccgt
    41401 gaagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg ccgtttatac
    41461 gccagccggg gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg tatgctgccc
    41521 tggcagcaac tgagcggcgt cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa
    41581 ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gcgcgaccag cacggctcta
    41641 agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt accaaaccgt
    41701 ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg
    41761 cagttaattg tcacggtgga taacggtatt tcctcccatg cgggggttga gcacgctcgc
    41821 tcgttgggca tcccggttat tgttaccgat caccatttgc caggcgacac attacccgca
    41881 gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa atcactggca
    41941 ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg cgatcagggc
    42001 tggtttgatg agcgtaacat cgcaattcct aacctggcag aactgctgga tctggtcgcg
    42061 ctggggacag tggcggacgt cgtgccgctg gacgctaata atcgcattct gacctggcag
    42121 gggatgagtc gcatccgagc cggaaagtgc cgtccgggga ttaaagcgct gcttgaagtg
    42181 gcaaaccgtg atgcacaaaa actcgccgcc agcgatttag gttttgcgct ggggccacgt
    42241 ctcaatgctg ccggacgact ggacgatatg tccgtcggtg tggcgctgtt gttgtgcgac
    42301 aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa
    42361 gagatcgaac aaggaatgca aattgaagcc ctgaccctgt gcgagaaact ggagcgcagc
    42421 cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca gggcgttgtc
    42481 ggtattctgg cttcgcgcat caaagagcgt tttcaccgtc cggttatcgc gtttgcgcca
    42541 gcaggtgacg gtacgctgaa aggttccggt cgctccattc aggggctgca tatgcgtgat
    42601 gcgctggagc gattagacac actctaccct ggcatgatgc tgaagtttgg cggtcatgcg
    42661 atggcggcgg gtttgtcgct ggaagaggat aaattcaaac tctttcaaca acggtttggc
    42721 gaactggtta ctgagtggct ggacccttcg ctattgcaag gcgaagtggt atcagacggt
    42781 ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg
    42841 tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct gcaacagcgg
    42901 ctggtgggcg aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg
    42961 gatggtattg cttttaatgt cgataccgcc ctctggccgg ataacggcgt gcgcgaagtg
    43021 caactggctt ataagctcga tatcaacgag tttcgcggca accgcagcct gcaaattatc
    43081 atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg tggattgggt
    43141 acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca gaccatgttt
    43201 gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga cgttcttagg
    43261 gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga
    43321 acagccggat gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc
    43381 cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag atgtttctgg
    43441 tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag ccgttgctga
    43501 actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt tctctggcga
    43561 atatgacagc gccgactgct acctcgatat tcaggcgggg tctggcggta cggaagcaca
    43621 ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa
    43681 aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg tgacgatcaa
    43741 aatctccggc gattacgctt acggctggct gcgtacagaa accggcgttc accgcctggt
    43801 gcgtaaaagc ccgtttgact ccggcggtcg tcgccacacg tcgttcagct ccgcgtttgt
    43861 ttatccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatc tgcgcattga
    43921 cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg
    43981 tattacccac atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa
    44041 gaacaaagat caggccatga agcagatgaa agcgaagctt tatgaactgg agatgcagaa
    44101 gaaaaatgcc gagaaacagg cgatggaaga taacaaatcc gacatcggct ggggcagcca
    44161 gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgcaccg gggtagaaac
    44221 ccgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag caagtttgaa
    44281 agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg acgcggtagt
    44341 cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc gcgagcaggg
    44401 gattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc acgcagaatt
    44461 cgacggcaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg ttgctggccg
    44521 catgatgacc cgtcgtatta tgggtaaagc gtctttcgtt accctgcagg acgttggcgg
    44581 tcgcattcag ctgtacgttg cccgtgacga tctcccggaa ggcgtttata acgagcagtt
    44641 caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aagctgttca aaaccaaaac
    44701 cggcgaactg tctatccact gcaccaagtt gcgtctgctg accaaagcac tgcgtccgct
    44761 gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc gttatctcga
    44821 tctcatctcc aacgatgaat cccgcaacac ctttaaagtg cgctcgcaga tcctctctgg
    44881 tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga tgatgcaggt
    44941 gatccctggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc tggatctcga
    45001 catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggttg gtggcttcga
    45061 gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac gtcataaccc
    45121 agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc tgatcgagct
    45181 gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg aagtgaccta
    45241 cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat
    45301 caagaaatat cgcccggaaa ccgacatggc ggatctggac aacttcgact ctgcgaaagc
    45361 aattgctgaa tctatcggca tccacgttga gaagagctgg ggtctgggcc gtatcgttac
    45421 cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca ttactgaata
    45481 tccggcagaa gtttctccgc tggcgcgtcg taacgacgtt aacccggaaa tcacagaccg
    45541 ctttgagttc ttcattggtg gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc
    45601 ggaagatcag gcgcaacgct tcctggatca ggttgccgcg aaagacgcag gtgacgacga
    45661 agcgatgttc tacgatgaag attacgtcac cgcactggaa catggcttac cgccgacagc
    45721 aggtctggga attggtatcg accgtatggt aatgctgttc accaacagcc ataccatccg
    45781 cgacgttatt ctgttcccgg cgatgcgtcc ggtaaaataa gcattacgtt atgctcacaa
    45841 ccccggcaaa tgtcggggtt tttttattta agctgggtaa atgcagataa tcgttttctg
    45901 gcttcgcgat ttgtcgcctg catcaccatc cacggactga acgcccacgg cgtggcatca
    45961 ataccgtgta atacatctgc taaatcacac cattgataat ccatcacttc atcatcattg
    46021 atctgtaacg cactagtggt gcgtgcggca aataccggac acacttcatt ttccacaatg
    46081 ccactcggat cggtggcgcg gtagcgaaag tcaggataga tagattcagg aggcgtaatt
    46141 tccacgccaa gctcataacg gcaacggcgg atcactgcgt cttcgttgct ttctcccagt
    46201 tgtgggtgcc cacaaaccga gttagtccac acgccaggcc atgctttttt gctcagtgcg
    46261 cggcgggtaa ctaataattg tcctttggca ttaaacagcc aactggagaa cgcgagatgt
    46321 aagcgggtgt ctgccgtgtg tgcggcatac ttttccagcg tacctgtggg aactccctgt
    46381 gcattcaata aaatgacgtg ttccgtttgc ataatttctc acatgtaatt ctgatcactc
    46441 atcattatag ataattgaag agtgaaatga ttaaccaagt gagcgtttat cgacaaccgc
    46501 ccgttttgag cggatgccga caggtaaaaa ccatttaaaa aagtagcatc atatatttga
    46561 agcgactttc aatggccatt gcaacacagt cagaaatgac aggaatagag agagtaaaaa
    46621 gcaacaacag actgaatata gcgcgcatta aaaaattcat attattctcc atgctcattt
    46681 ttcttcagct tgcgcatcag aagccacata cgtactgtcc tgacttttaa atctttatca
    46741 gagacggatt ccttcaccag gtcagcatga tgctgatagc cattaaagaa gacgttcagc
    46801 acaacggcgc taagggttgc gagcataatg ccgctatgta gcagcggttg cagtacggcc
    46861 ggtaacttag aaaagaaatc gtgagagagc gtcggagtca tgccaacgcc gagactgatc
    46921 gccacaatat agaggttgta acggttggtg gtgtagttac agcgcgacag aattcgaatc
    46981 cctgtcgcca gtaccatgcc gaacatcact agaccagcgc cgcccagcac aaattgcgga
    47041 atggaggcta ccagcaccgc catttttggc accatgccaa acaggattaa tataattccc
    47101 gaagaaatac acacccagcg gctatgaacg cgcgtcacgc taaccaggcc aacgttttga
    47161 gaaaatgacg tgtgggggaa gctgttaaac gtgccgccta tcattgtccc tacgccatcg
    47221 acacgcagcc cgcgaataat atcgtgcgaa gagagtttac gaccgactat ttcacccagt
    47281 gccaggaaca tccccatcga ctcgataaac acgatgatta acacggcagt catggtcaga
    47341 atggaaacgg gatcgaaaat cggcatacca aacgacatcg gcgtaacaat cgcaaaccat
    47401 gaagcatcat gtagcccgga taaattgact tcattcatca tccacgaaag taaaaagcca
    47461 aatacaatcc ccagtaatac ggcgacgttg gacataaatc ctttcgcata gcgagtaatg
    47521 agcaagataa aaattaagac ggcaaaggag atacctaaat aaacgggatt accatattgc
    47581 ggattccctt tacctccggc ggcccagtca atacccacct gaatgatgct aagcccgata
    47641 gaagtaataa ccacaccggt aaccagtggc gggaataaag gcatcaagcg accgataagt
    47701 ggcgctaata atgtggtgat aaaacccgcg gcgatagtgg caccaaatat ccccagcagg
    47761 ccgatatccg ggttcatccc aatggctatc attggtgtta cagcagcaaa ggtcaccgac
    47821 ataatcaccg gcaggcggat ccccataaag cggccgatac cgatacattg caataatgtg
    47881 acgatcccgc agcaaaagag atccgagcta atgagcatcg caatagcttc ttttgagagg
    47941 cccagtcggt caccaatcat aagaggaaca gcgactgcac ctgcgtacat gacaaggacg
    48001 tgttgcagac cgagtatgat cagctttcct ggtgataata tgcggtcaac ctcatcagtt
    48061 gggcggtctt gcccagaaga tgagggaagt tgggaatcta tggcgctcat cagtgtttct
    48121 cctttcgccc gccagagtaa aaagcaaccg tttacgttaa tgaaactgtc taaacacatg
    48181 ctcgtggagt gccagttttt gcgaggaagg ggagttagag acaggaattg cggaggcaat
    48241 cacataatcg tggttaaacc acgaaaatga gaagagaacc tgacaatgga attatctgta
    48301 attttctcat attgatattt cattaatcat tctggcatgt cgcactctcg catttaatcg
    48361 tttttatctg gatagcgctc ttttgatcgg caatgttgaa ataatgaaag cggcatagat
    48421 tgaggggcaa taatttgaac tcagtcgcaa aattataatt aaaacggtca tgctacctaa
    48481 tatatgcccg tttattgcat cgcgaaattt gagaagacat aactcattaa atgaaaggga
    48541 tgcaaatgaa taagtttatt gctgctgaag ctgcggaatg tataggctgt catgcttgtg
    48601 aaattgcctg tgcggtggca cacaatcaag aaaactggcc gctgagtcac agtgactttc
    48661 gaccgcgtat ccacgttgta gggaaaggcc aggctgcgaa tccggtggcc tgccatcact
    48721 gcaacaatgc cccttgcgtt acggcttgtc cggttaatgc tctgactttc cagtccgata
    48781 gcgtacaact ggacgagcaa aaatgtattg gttgtaaaag atgcgcaatc gcttgcccct
    48841 ttggcgtcgt tgagatggtc gatacgattg cgcagaaatg cgacctttgt aaccagcgca
    48901 gttccggcac gcaagcctgt attgaagttt gcccaacaca ggcgttacga ctgatggacg
    48961 ataaagggtt gcagcagata aaggtggccc gccagcgcaa aacggcagca ggaaaagcgt
    49021 catcagacgc tcagccatct cgcagtgcag cgttgctccc cgttaactcg cgtaaaggcg
    49081 cagataaaat ttcagcgagt gaacggaaaa cccactttgg cgaaatatat tgcgggctgg
    49141 atccacaaca agcgacttat gagagtgacc gctgtgttta ttgtgccgaa aaagctaact
    49201 gcaactggca ttgcccgctg cataacgcta ttccggatta catccgtctg gtacaggaag
    49261 gaaagattat tgaagcggca gaactttgcc accagaccag ttccttacca gaaatttgcg
    49321 gcagggtatg tccacaggac cgtctttgtg aaggcgcatg tactttgaaa gatcactcgg
    49381 gcgcagtctc tatcggtaat ctggaacgct acatcaccga taccgcgctg gcgatgggct
    49441 ggcgtcccga tgtcagcaaa gttgttcccc gtagcgaaaa agtggcggtg attggcgctg
    49501 ggcctgcagg gttagggtgt gctgatattc tggcgcgcgc aggagttcag gtcgatgtct
    49561 ttgatcgcca tccagaaatt ggcggtatgc tgacttttgg cattcctcct ttcaaactcg
    49621 ataaaacggt attaagccag cggcgagaga tattcaccgc aatgggaatc gactttcatc
    49681 ttaactgtga aattggccgc gatattacct ttagcgattt aacttctgaa tatgatgcag
    49741 ttttcatcgg cgtggggact tacgggatga tgcgagcaga tctgccgcat gaagatgcgc
    49801 ccggtgtcat tcaggctcta ccgttcctga ctgcccatac ccgccagctc atgggattgc
    49861 cggagtctga agagtatccg ctgacggacg tggaaggtaa gcgagtcgtg gtattgggcg
    49921 gtggcgatac gacaatggat tgtttgcgga cttccatccg cctcaatgcc gccagcgtga
    49981 cctgcgcgta tcgtcgtgat gaagtcagta tgccgggctc gcgcaaagag gtggttaatg
    50041 cgcgcgagga aggtgtcgag tttcagttca atgttcaacc gcaatatatc gcttgtgacg
    50101 aagatgggcg tttaactgcg gtgggcctga ttcgtaccgc catgggtgag ccggggccgg
    50161 atggtcgccg tcgtcctcgt ccggtagcgg gttcagagtt tgaattgccc gccgatgttc
    50221 tgattatggc ctttggtttc caggcgcatg ccatgccgtg gttgcagggc agcggaatta
    50281 aactcgataa atggggcctg attcaaaccg gtgacgtcgg gtatttacct acccagacgc
    50341 atctgaaaaa agtctttgct ggtggtgatg cagttcatgg cgcggatctg gttgtcactg
    50401 caatggccgc aggaaggcag gcggcgcgcg atatgttaac tctgtttgat acgaaggcat
    50461 cgtgatgaaa tcgttaatta tcgttaatcc ggctgactgt attggctgcc gtacttgtga
    50521 agtagcctgt gtggtcgctc acccttcaga acaggagttg aatgccgatg tctttttacc
    50581 ccggctgaag gtacagcgac tggacagcat cagtgcgccg gtgatgtgcc atcagtgtga
    50641 aaacgcccct tgtgttggcg cttgccccgt gggggcgctg acgatgggcg agcaggtggt
    50701 gcagaccaat tctgcccgtt gtattggctg tcagagttgc gtcagtgcgt gcccgtttgg
    50761 aatgatcacc attcagtcgt tgccggggga tacccgacaa caaattgtga aatgcgatct
    50821 ctgtgaacag cgggaagagg ggccagcctg cgttgaatct tgcccaacac aggcgctgca
    50881 gttgcttact gaaagagaac tcaggcgagt ccgccagcag cgtattgttg ccagcggtga
    50941 gaatcctctc tgaaagaaat ctccgcgctg aaaggcgcgg agaatctatt agattgccca
    51001 accacccgcg taaaatgcga ccagtgcggc agtaatgatc accgtaccaa tattcagctt
    51061 ttgccattcg cgtgcaaaga cgcggcctac gaccagtgtc acaaagccca gcataatacc
    51121 ggtaacgata ttacaagtca gaacgatgaa cacggcacac accaggccag ccatagcgtc
    51181 aataaaatca ttgaaatcca gcttcgagac gttacttaac atcagcaaac ctacgtacat
    51241 cagagcgggt gcagtggcgt aaccagggat caaaaatgac agcggtgata aaaacagaat
    51301 taacaggaat aacgccccca ctacggttgc ggttaaacct gttttacccc cggcggcggt
    51361 tcctgccgct gattcgatat aaaccgctgc gggcgctgcg cccaccaggc cggagaatat
    51421 tgaacttact gagtcactgg tcagggcttt gccgccgttg atgatctggt tgtctttatc
    51481 caacaaattc gcctgaccgg cgacggcacg gatggtgcca gtagcgtcga acactgcggt
    51541 catcaccaat gccagtacac tcggaagtac agttggctgg agtgcaccca taatatcgag
    51601 gctgaaaatc agagacttac catcttcgcc agtcaggctt ggcatcgcca ccagaccgtg
    51661 gtatttcacc gctgggtcaa agattaagcc gatgatcgaa attgcaataa tcaccaacaa
    51721 gatcccgccg ggtacgcgac acttctccag gccgaagatg accgccagcc ccagcaagct
    51781 catcatcacc gggaaggagg taaacgcacc gagcgccact ggcaagcctt caatcgggtt
    51841 tttgataacc atacccacac cgttagcagc aatcagcagc agaaacagcc cgataccgat
    51901 acctgtaccg tgagcgatac ccatcggcaa attacgtaag atccaggtac gcacaccggt
    51961 tacggaaatg gcggtgaaga tgacgcccat cagaaatacc gcgcccagtg cgacaggaac
    52021 gctaatttgt tgcccgagta ccagactgaa tgcggtaaac gccgtcaagg aaatcgcgca
    52081 accaatcgcc attggcaaat tagcccataa tcccatcagc aacgagccga agcccgcgac
    52141 cagacaggtg gcaacaaaca cagctgcggg aggaaaacct gctttgccca gcattcccgg
    52201 aacgacgata acggaataaa ccatggccag aaaggtcgtt aagccagcca gtacttcctg
    52261 acgaacggta ctgccacgag cggtaatttt aaaataatta tccagcgcgc cctgtggctt
    52321 tggtgcgtcc ggtgtttgta ggatgtctcc agacataatg atgtcctcac gaggatgtta
    52381 aagttttatt attagttgcg ttcgtacacc agacgaccat caacgtaggt gcggtagatc
    52441 gaacggtcat cgcccaacgt catcatcacg aacaatttgt cgactaaaga aacagagttg
    52501 tcatagcgca gctgctgtag cggagtggcg gtgggttcca tcaccacgaa atcagcctct
    52561 ttgccaggta aaaagttgcc aatcaaatcg tcaaggccca gagatttcgc tccgccgagc
    52621 gtggccaggt aaaacgcttc atatgccgag aggcgatagc cttgtaattg caatactttg
    52681 taggcttcgt tcagcgtttg cagcatgttg aaagtggttc cggcaccgat atccgttccc
    52741 atgcccactt taactttctt ctgccatgct tttttcaagt tgaataagcc gctgccgagg
    52801 taaaggttgg aggtcggaca gaaagcaatg ctggatttgg tttcgctgag acgatcccac
    52861 tctttttctt cgagatggac gcagtgagca aagacacagt ttttaccggt caggccgtac
    52921 tgatggtaaa catccagata accatcatgg tcaggataaa gcgatttcac ccaggcaatt
    52981 tcatctttgt tttcacagag atgggtatgt acccacgtat ccggatattc ttctttcagg
    53041 cgttgcgcca tcgccatctg ttcaggagat gaggtcgggg cgaagcgtgg cgtaatcgca
    53101 tatagcagac gaccattttt gtgccagcgt tcgatcagtt ctttgctttg gtgatagctg
    53161 ctttcggcag tgtcgagcag ataatccggt gcgttgcggt ccatcatcac cttaccggca
    53221 atcatacgca tattgatatg actggcggct tcaaacagcg catcaacaga ttgcggatga
    53281 acagtgccaa acaccagcgc cgtggtggtt ccgttacgta aaagctgctt gatgaagaac
    53341 gccgacattt cgcgggcgta ctctaaatcc tcataacgac gttcagtagg gaaggtgtgt
    53401 ttattcaacc actccagcaa ttgctcacca taggccccca ccatttcact ttgcggataa
    53461 tggatatgtg tatcgacaaa gcccggtact atcagtttgc cgcgatagtc gcgcacgcga
    53521 atggtgtcag gaatttgatg ctttccgttt tcccattcgc caaaccattc cacttttccc
    53581 tgtttaatga gtaataaacc atcctcaata aaccgcagcg cagaggcaat ctcttccggg
    53641 ttatcgatcg tacgggtgac atcaataaaa ctgcctcgta ccgcttttaa cgtgtgttct
    53701 cctgacatca ttgactcctt tactgttaat ccatctcttc cgctgaggta ataccaggca
    53761 gaacgttttc ctgtcggtag ccaccaggga ggataatatt taataaaatc gcagttaacc
    53821 cgccagcaca aatagggttt tcaactaata cataaataga ggctggcaat attttaaaaa
    53881 tttcgggatc gtaggagacg ccaagcccta aacctaaaga agtggcgaca ataagtgttt
    53941 cacggcgctt taaaccgttg gtgatgatga tgcgaatccc tgcgatggca atcatggaaa
    54001 acatcaacgt cattgcgcct cccagaactg ccgagggaat ggtcgtgaag aagccgccaa
    54061 tcatcggaaa taagccgagg ataaccagca ttaccgcgat ggttcgcccg acataacgtg
    54121 aagcgacgcc agtcatctga ataaccccat tattttgcgc aaacgtggtt aatggtaatg
    54181 aaccgacagc ggaggcgata acagaaacca gaccatctgc cagcacgccg cctttcagcc
    54241 gggactgata ctcttccccc tgaatggggc ggcgggaaac cattgccgtg gcggtgatat
    54301 cgccgactgc ttccagcacg ctaagcagat aaatcgtgcc aaccaccagg aactgatgga
    54361 agctaaaact aaagccgtat ttgaacggat gcgggatggt gattaacggc aaattgcgca
    54421 tactgctgaa atccaccatg cccaggcata acgatgcgat atagccgaca catagcccaa
    54481 tggcgatccc tcccatgcgt agcaacggac tgcgacagca gttaaagccg atcaccacaa
    54541 ttaaaaccaa taaaccaacg ccgagatgtt cgtaattgcc gaacgtaccg ctgcttttgg
    54601 ctgcaaatcc tccaccaaaa tcgataatgc cgactttaat caggcttaag ccgatcatca
    54661 gtaccacaat accgctgacg gtaggcgtaa taacccgacg taaatagggc aagataaatg
    54721 aagatccgac aaccagaaat gcgccaacga aggagacgcc gagaagcgac gacatgatta
    54781 actcttcgtg aaaaccgtcg cttttcatgc tgctgcccag cgcaatcatg accgtaacaa
    54841 atgaaaaatt gactgactga attgagagta ggccagaacc gacgatgccg tagcggttta
    54901 cttgtaacca ggtaccaata ccagaggcga tcatcgccat agaaacaaga taggcagttg
    54961 tttcagcgga aagctgtaag gccgcaccca cgattaacgc gggggttacc atcggaacga
    55021 aaattgccaa cagatgggta atggcaccaa ctagagcctg atgaaaggga gggcgatcct
    55081 ccagttcaaa tataaggtca gaacctgcat ggtttatatc agacatccct tcactccttt
    55141 taaagtaaat ttgatcatct gaattagtaa aataaattcc cacgaatttg gggcgcagga
    55201 cgccttgaga acggcttatc tcattttcgc agtcactatt tagtcatcgt tcaaacagag
    55261 aaacaggccg ccgaagcggc catttattat ttatattttt tccagcgcag tgagtatttt
    55321 ctccggtgtg aaatgccatt cgcgtaacca gatgccgcat gcatcgtgaa ttgcggtagc
    55381 aatcgccgga gctgcgccat ttacaccgat ttccgagatc gatttcgccc cgaacgggcc
    55441 gactttatcg tcgctcggca ccagcacagc gcggaaatca cgcggaatgt caccaatttt
    55501 cggtgcgccg taactgcgta aatcacgcgt taacgggtga ccttcggcgt cataaatgat
    55561 ctcttcgctc atactgtggc cgatagcacg cagggtggca ccgtagattt gtcccaacgc
    55621 taactctgga ttgaccggtg taccgcagtc cagcaaggcg tagaatttat ccaggcggat
    55681 ttcacccgta cgcgtgttga cggcaacttc agcgaagttt gcgccatacg ggaaggcgaa
    55741 atcaggcgtg atataactgc cagttcccac cagtgaacca aagccggtgc cggtttcgcc
    55801 tttatgggca atatccccga aactaacttc gcctttcttg ccgcgcacga cgcccggcgt
    55861 tgccagttga acatctgcca ctggctcacc caacatttgc gcgccgtgga acagaatttt
    55921 ctcccgtaga ttttccgctg ccaaacgcgc cgcgttaccc gagaagcaag taccggacga
    55981 ggcatatgcg cctttatcaa acaacgcatg atcggtatca ccggagataa catgcacgtc
    56041 ctgcggtggg cagtgcagca cttctgctgc cagtttcgtc actacagtat ccagaccagt
    56101 accaatatcc gcaccgccag aatgaacgat aaaggtgccg tccgattcca gtttgatcat
    56161 gcagttagcc tgatcgatat ccgggatacc cgatttctgc atgataatgg cgacgccgcg
    56221 accgatgtgc cagtcaccat tttgcggttt cggtgacgac cattggatca tctcgcgacc
    56281 ctgacgcagg atctcttcca gtgcacagct ggcggcggaa ggaacggagg tcggcgcttt
    56341 accttcaccg attgcaccga gaattttcag ctcttgccct tcgtgtaccc ggttacgttc
    56401 gataatttcc agttggtcga tctgtaactg ttcagccagt tccgctaatg ccatggtgat
    56461 agcgaagtta cctttcggtg cgccataacc ctgataagca ccatttgggc aaatgttgct
    56521 gtagtaggtg gtgacctgga aatcgacgtt atcgcacgga tataacggca gcgacagcgc
    56581 cggtccgtta cacggtacgg tgagtgagtg gttgccgtaa gggccagtgt tggcgcggaa
    56641 atccatcttc actgccgtca ggcgaccatc ttttttcgct cccagtttga cggtgacttt
    56701 cgcgacgtga cgagaggtgt tagcaataaa ctcttcttca cgggtgtagc ggaacagtac
    56761 cggacgcccg gtcacgcagg ttgcccaggc gcacacttct tccagcagga tgtcctgttt
    56821 ggaaccaaaa ccgccgccaa ctcgctcttt aatgacatga actttatgct gtttcatgcc
    56881 cacgaggcgc gcgacctggc ggcgtaagtg ccatggtacc tgggtggagg cgtggataac
    56941 cagacgatcg ccgtccatac gagtaaagca gatatgtgtt tcagtcgggc actgctgcgc
    57001 ttgcgttgag ttataggttc gctcaatgat cacatcggca tcggcaaagc ctttgtccat
    57061 atcgccaata tgaccatgaa tactggcggc gatatttttg cgagggcgag aaccgatcgg
    57121 gaagttgatg atcatatgct cgccgcgctg ggctgcatgg ctgttatcgt cttccagagt
    57181 atctggcgca ccagcaacat acaccaccgg ttcatcgtgc acgacaggcg catcttccgc
    57241 cattgcttcg tcgatcgaca ttaccggctt aagcacttca tattcaacgt cgatgagctt
    57301 cagtgcttcg agcgcaattt cttcactttc tgcgacgacc gcagcaacac gatcgccgac
    57361 gtgacgcatt ttcttgccga acatacggcg gtcaagcggt gacggttccg gtgcgctctg
    57421 accacccggg gtatagtaga tatccgggca attcaggtga gtaataacgt gaacgacgcc
    57481 cggtaaggct tcagctttgc tgacatccag atgagtaatc agtgcgtgag cgtgtgggct
    57541 acgtaacatt ttaatgacgc aggcgtcagc cgttacgcgg tcttcaacat agcagggttt
    57601 cgcctgcacc attttcgcgg catcagtttt aggataatgc ttaccaatga cttctaggtc
    57661 gtcacggaaa gtcggagcga tatcaatggt ggcctgcgga ttatttttac gtgcaaccgc
    57721 cagttcaatg acctgatagt attgctgcca gccagcatcg cggctgaaca aaccagaaag
    57781 cgcgtcgtcg atctcttcgc gggtaggtgc ggcgatgcga tcgagaagat cggtgataat
    57841 cagagctgca gctggatcgt tataaccaga ctgcaccacg ccaacatcaa ccattgcctg
    57901 ttgaaccaga cttaactcgt tccatttgcc cagagattct gcggtacgaa tatctgcctt
    57961 ctctaactgt gcggcaataa gcaaggacgc gttaacgata ttaccgttaa agattattgc
    58021 gtcagaaccg gcaaacccga aaccatcatc actgttgcgt acagagtgca ttcccatgtt
    58081 aaacaacagc ttttgcacgt tttcgcctgg attaacggtt agctcctgag gcgcgccatt
    58141 taaagtaaag ttgatgatca tacggcttcc tccccagcct gttggcagtc ggcatacagg
    58201 tcggctacca gaactcccgt gatatagcgt ttataggcca cgctgccccg caaatcttcc
    58261 tgcgggaaaa tggcgttggc gacagcctgt tcaagtgcat tgccttccag attttgtttt
    58321 tcgacatcat gcagacgcag tgctttactg gcgacgccat ccagcgcaat tcgcataccg
    58381 tcgtggtctg tcattgcaac ggctgccgtc acgacggtta aacctgcctg agaacggcta
    58441 attttgcggg tcgcacaggt gcgatacgga tctttaatga taatttcggt tagcaggcga
    58501 tcgcatgggc aggccaggta gtcctcgatt gacagcgttt cgccgttgcc aaaaaccagt
    58561 tcagcatcca gtgccaacag gacgggaagc agcaccgact cttcctggcg ggcggcgatt
    58621 tcaccaccaa tggtcgactg attacgaaca tggcgtgagt aaacaaaacc gagggcttca
    58681 cgcagcgctg caggaataaa tcgcgcatca cgcagtggct gcaagcgaga cattgcgcca
    58741 atccgcagtg caccgttatc ccagtcaacc cagtccagtt ccagatcctg taaggaaatg
    58801 gcaatctttt tatcggtacg ggttggtgta gcattgagtt tgctgccccc ggcgaaccag
    58861 acggcttcat cctggtagcg gcgcttcagt tccagcgcct gttcgacaga gtcgggcctg
    58921 aaaaattgtt caatcatctt ctttccttat ttaaccctct tctctgcggg aagagggcat
    58981 ctgtcattta tgccagcgca tccatccgac gccacatact ggcagcggct tttctggctt
    59041 gtgcataaat ggaatcgcaa tcgaagttaa actgacggtc ttcatagacc atcacaccat
    59101 tgaccatcac gctgtgaacg ctgcctgagc ccataccgaa agcgatatgc ccggcgatat
    59161 tgtctgccag cagcggcgtc ggcgagttgt aatcgcaaat ggttaaatca gctttgtaac
    59221 cggcttccag aagcccaaat ttcgcgccaa agttgcggct catcagttcg ttaccgttag
    59281 tcagggcttt ggcaaaactg tcaggccaca gcggaccacc cgcatcgcga tgtttaaaga
    59341 aggcaaattt catctcttca aacatgtccg aaccaatgcc gtccgttccc aacgccagat
    59401 tgcggatgtc gctaaggtga tggttgtagc cgacatggtt gttcatgttt gaacgggcgt
    59461 tatgcaccag gaacgcatcg cgctgattga gtagggtgat gtcatctttc gacaagtaca
    59521 gcccatgagc gaccagcgtt ttgctgtcga tgagatcgaa ttgcgccagt cgtgccagca
    59581 ggtctttgcc gtaccagtgg tgactgtagg aaacgtcgta aaggtcttcc gcagcgtgaa
    59641 tatgcaaacc acggcctgtg gctttcacgg cttcacgcag catctccaga ccggcatccg
    59701 gcacggtaaa cggcgcgtga gcaccgatat gtgcttccac cagatacggc tcgcttgtcg
    59761 ctttcttcgc ctcatcaatc aaacgggcga aacggatgtt ttcttctaca ccttcctgca
    59821 actctttgat gccgttgtta cggtcagtag tttcaaaaca ggtcatcgcg cgcaggccaa
    59881 cttttaaaaa tgcgtcgcgc aatgtggaga gcgacccgcc gatatacgcc ggagaggcat
    59941 ggtgatcgat aaccgatgta catccgctct taatcgcttc cagggaacaa atcagtccgc
    60001 tgtaatagag cgactcttca tcaagggcgc gatcgagccg ccaccagaga tttttcagcg
    60061 ttgagatgaa atccgggcaa ggggcgatgt ttgccataat tccgcgggaa agccccgagt
    60121 aaaaatggtt gtgcgagcag acaatccctg gcatcacaat ccggccatgc atctctttga
    60181 agctggcgtc ggggtagcgt tgcgtcaggg catcgccgat agcgacaatc acatcgtttt
    60241 cgatggcgat atcaacgcct tcctgcactt ttgccgggtg tagctgtacc gcagtgacat
    60301 tcttcagaat caacatgatt acacctccac gcggcccagc agataatgat gatgctggtg
    60361 gacatggctg atgatgcggc acatatcgtt cagctccggt ggtacgttgt taaactgacc
    60421 tttgctgtcg atgtttaaca cccagctttg gttattcaga cgtactcgta cccggcaatc
    60481 ttccacaagg aagcctgggt tgctgctgtt atcaaagtct tgcgccaggc tgaagacggt
    60541 gattttgtct ttgtacggtt taccgttcca cggacagaac tgagcgcagt tgccgcattc
    60601 gttacagtaa gcgtcgaggt gcagcgtctg gaaacggttc tggaagcctg ggaccgcaat
    60661 ggagacgttg gcgcggttcg ggcagacatc cacacacttg ctgcaaacgt agttacattc
    60721 gaggcagcga gcggcttcct gggcgacaaa cgcgtcacga tcgtcactgt tcaccagcgt
    60781 gatagagata tcgcctttac gttgatagat ttccgctgga ttgacgttgt tccagtattt
    60841 atcgttctgg tgggaacgga tattttcccg actaaggatg gcatcggtcg cccgacgcgc
    60901 ggttccgaca gcagcgacaa tggaggatgg tccgcgctgt acgtcgccga tcataaagac
    60961 gtcagtcaga cgagtttcgc cattatggtc gacgtctggc cagccgtttt tgtccagcgg
    61021 cacgcccatc gcattcaggg cttcagtatc ctgctgttca ccaatggcgg tgatcaggct
    61081 gtctacaagc agtgttactg tttcattggt ttcaaccgga cgacgacgac ctttctcatc
    61141 cggttcgcca agcgacataa cgcgcaaggt taaggtgcca tcagcatcga aacgttccgg
    61201 attattcagg aaacggaact ctacgccgtc gtgcaacgct tcttcatact cttcgcgcca
    61261 tgcgggcatc tcttgtagtg aacgacggta aacgatcgtt gctttttcta cgcctggaac
    61321 gcgtaacgcc gcacgagcgc agtccattgc ggtgttaccc gccccgacaa cgaccacatg
    61381 tttgcccagc ttgagcgctg tacccttgtt gtattcacgc aggaagggga gtgatttcca
    61441 gacattttgg ttgtcgcccg ccagtttcac accgctattt ttatcagtgc cggtggcaat
    61501 cagaacatag tggaagccct gattttttaa ctgctcaatg gttaaatcgg gtgagcagcc
    61561 atactcaaat ttcacgccgt gagcggcaac aaaatcgata tcgtgctgaa ttaactctgc
    61621 aggaatacgg aactgaggaa tgatattttt caccacgccg cccgcattgg cttcgcgttc
    61681 aaacagcgta accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg
    61741 acctgcacca atcacggcaa ccggatggcg tgaaccagaa ccggctggtt tgtgccagcg
    61801 ttgcttatat tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag
    61861 cgcactgtcg taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc
    61921 ggtaatggcg ggcagagcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg
    61981 ttcgccaagc agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca
    62041 gggggcaacg tagcagtcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg
    62101 cttccagtgt ttctgggtgt attccatggt taacgcatct gctgccagtc tgttcagtcg
    62161 ttcgacgtca acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc
    62221 acttaagcgc agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat
    62281 acctgtatca aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg
    62341 cagtttgccg tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag
    62401 cgcacggcct gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc
    62461 gagagtgtta gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtgccatcag
    62521 gcgttccagc atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt
    62581 taagcctatg taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag
    62641 taaggtcggg ttaagtttca caaaggtgtt gagccctttt tcttccagca tgtagcggca
    62701 aatggcttcg atttcatgcg gaggacagcc gtgcatggtg gagagggtga ctccatgcac
    62761 catactggtg gggatgcgag cgggtaaggc ttgcaagctt tcgcgttttt cctgcaatcc
    62821 gtggcgagct aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc
    62881 gaatttcgga tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg
    62941 cttaatacct tcgaggttgt aaccgacgct catattaaag atgaacgatt taccggaatc
    63001 agaaggctgg aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc
    63061 ccaggcttta agcagggtaa attcggtaga ccattcggtg ttaaagcact cgtcttcggc
    63121 atcgatacag ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat
    63181 gaagcgtccg ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc
    63241 agcggcaggg ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt
    63301 tacaggtgag taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc
    63361 atcaaatatg cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac
    63421 cctctcctta gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta
    63481 gggggataga gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg
    63541 tgaggcggct cgaaacttaa gggctttttg tgaatgaact cacaggtgct aaatgagaat
    63601 taaaatcact cctgaaaatt attattgcca gcccatttat caatttttct cttttctcaa
    63661 aatcagtgat tccttatcat tatgataatg agtgccggaa ggattaaata tttgaacgca
    63721 atcgtttaac tacgttttct aaatttcagt atacctttcc ttcgctgtaa taaagtcgtc
    63781 cggagtatca atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc
    63841 cataaggagt gcctgacgca tattggtgac attgggtcgt tggattgcct gcatcaggca
    63901 tggttttgac actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc
    63961 atcatttcgt aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc
    64021 gtgggtgaga aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag
    64081 taaaccctgc gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg
    64141 ttcgtgcagt tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa
    64201 cgcatttttg atacttgtat caagaattgt tccctgttcc cagggtaaca tcattttcca
    64261 ttgccccatt cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt
    64321 ggttccatta tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta
    64381 agttggggat aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca
    64441 tcggcattag tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt
    64501 gctgggggga aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg
    64561 cgtgtgttaa tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt
    64621 ttctgtcgtg atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt
    64681 tttcacagct ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat
    64741 gcactggtgc aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga
    64801 atgccgttaa aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg
    64861 attgctgtga tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc
    64921 tggtcgcagt ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat
    64981 ctcgttgcgc tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg
    65041 cgagtctggt tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc
    65101 cttcaaccgc tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat
    65161 cctgcaatcg cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga
    65221 tatccattga ggttggcatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt
    65281 gtccgtttgc tatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac
    65341 aaatgttagt gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc
    65401 ggaaggtgat tgaagagtcg cttcaggcat tgcaggaacg taagccgcga ttattccatg
    65461 gacgtatggc tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag
    65521 tatttatcag cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca
    65581 accgggcgat agcccagagt gcggcgctat taggatttga tattgccgtt gctgatattt
    65641 atcgcgaaag cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt
    65701 cgtttggtgc ggcagtggaa gcactggata ttcgccctga taattttgtc ctgattgcca
    65761 cgaataatca ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg
    65821 ggttgctggc aagtcgtcgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg
    65881 tggctgaaga acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa
    65941 cgccgcagga gatcgccatc agcgtgctgg cagaaatatt acaggtgaaa aataacgcgc
    66001 cgggtgggct gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg
    66061 gtgcgggaga tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga
    66121 tcatgctgga agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg
    66181 tgttcgatgg cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg
    66241 aagcgatgaa acttaccgaa cgcggattca ttcctgtgat ggtagatccc gcctgttcat
    66301 tgcttgatga actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg
    66361 gaacgcgggc agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgctggga
    66421 aggattgtca tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact
    66481 ctggttgtgc gcaggagaat accggtgttc ctggcaatat tatggggcat accacccgac
    66541 gggtgatccg tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg
    66601 tgaaagaggg cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg
    66661 ggatggtgcg tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg
    66721 atatcgatcc tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga
    66781 ttggcggcgg cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa
    66841 aagaagtgct ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctga
    66901 gactgatgac aaacgccaaa ttgcctgatg cgctacgctt atcaggccta caagtgcatt
    66961 gcaatctatt gaatttgcga tgttttgtag gacggataag gcgctcgcgc cgcatccggc
    67021 atcaacaaag cgcacttact tgacaatctt tggccggatg gtattaccca cccggccttt
    67081 aaattactga caaatcaccg ttcctgtttt gccttcaatc ccttctttcg ctttgctcaa
    67141 tacggtaatc agtgcttcac gacctgcgcg agagcgagca aacgaggcgg cagcttccac
    67201 tttaggcagc atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact
    67261 taatgacagg cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc
    67321 taccgcagtc agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc
    67381 ccagtcttta tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg
    67441 gataccacca ccgccaacgg tgatcaccac ctgacctgca tctaccagag ctttaaccgt
    67501 ttctttttca atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc
    67561 agcatcttct ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa
    67621 gaacgaaccg atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac
    67681 ctgagtaacc agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa
    67741 agcgttttgt agatcgtaac caatataacc ctggcttaat gcaacacaga cagacatcgg
    67801 cagcattggc gagtgcgctt cagtttttgc ggcagcttca aaggcctggt taatcatgcc
    67861 cacctgcggc ccgttaccat gagtgacgat aacttcatgt ccctgggcaa ttaaatcaac
    67921 aattgcctga gaagtaattt ttaccgcttt catttgcccg gccaggtcat cgcccagcgc
    67981 attcccgccc agggcgagaa caattttctt actcattttt cctcactcat cattcttgat
    68041 tgtgtataga tccctggcag tggttagagc acgggaggga caaacggttt gcgtcgcagg
    68101 aaacgacctc gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg
    68161 gataaggttc tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg
    68221 ttgtcgtgga gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg
    68281 tcgccgtcgg aaccgggcgc taataatcct ttttgcggcc acaggccaaa caacctggcg
    68341 ggcattgcgc tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca
    68401 ctggaaaaca gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg
    68461 aaatcgcctt tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggttgcc
    68521 accacgtcaa tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta
    68581 cgcagcggtg ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc
    68641 aacaggagat attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga
    68701 cgcagataat ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg
    68761 gcaatttgtg ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga
    68821 ctcaaggcat gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg
    68881 gcgatagccg catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg
    68941 cgtaatgcct gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta
    69001 aaactgctca gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg
    69061 tgctggatca caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga
    69121 taaacctcca gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata
    69181 atggttgttg taccgccaca cgcagctgcg cgggtaccgg taaaaaaatc atcacaactg
    69241 cgcgcgatgc cgacatcaat attgaaatgc gtatggacat ccacgccgcc agggaaaacg
    69301 taacagccag tggcatcaat ttcttcatac gggagctgcg gcgaaatatt gttgcccaac
    69361 tggcgaacaa tcccgctttc aatcagcaaa tcctgtttgg cttgtccatc tgcgttaacg
    69421 acagtgccgt ttttgatcaa tacgcgcata gcaaactcca aaaaccctcc ggcgaaccgg
    69481 aggggaataa gtgaagagta attactctgt tgccagccag cttaacggga ttgcagcgta
    69541 catcgcggca caggtcacca ggtgagattt ccaggttttt tcgttaggtg catgcgcttc
    69601 aggttcttta cccgggccaa agccgatcac cggaatgccg tgacggccca tgatagatac
    69661 gccgttagtt gagaaggtcc acttatcaac aaccggcgct ttgccaaaca gcccttcgta
    69721 ggcattcacc agtgctttaa cggtgaagtg atcttcttcc actttccagg tcgggaagta
    69781 gcattcggtt gggtaaacca ggccagtcca ggacggacgg tcgtagttgt acatagaaac
    69841 aaccgcgtta gctttctgta ctgcaggcag ggcgcggatt tcgtccagcg cgccttccca
    69901 ggtttcgccc caggtcagac gacggtcgat tgaaactgcg cagctgtctg ctacagcgca
    69961 acggcttggg gatgtgaaga agatttcaga aacggtgagg gtgcctttgc cgaggaattc
    70021 gtcataaccc agacgttggg agagttcttg taattcgcca agaatcggac ccattttgaa
    70081 aatggcgtta tcaccgcgtt ctggtgcaga accgtggcag ctaacaccct gaacatcaat
    70141 acgaatttcc atacgaccgc gctgaccacg gtatacctgg cagtcggttg gttcggtact
    70201 gaccacaaat tccgggcgaa tgccggattg ttcaataatg tactgccagc acagaccgtc
    70261 gcagtcttct tcctgcaccg taccggtaac cagcagggta tattcatctt ccagaccgag
    70321 gtctttaatg attttaccgg cataaaccat agaggccatg ccaccttcct ggtcggaagt
    70381 accgcgacca ccaatcagtt catcagtttc catgccttcg tacggatcga agtcccagtt
    70441 tttgatgttg ccaatgccga cggtatcgat atgagcgtcc attgccacca gacgcgggcc
    70501 gtggccgata taaccgagaa cgttgcccat cgggtcgatt tcaactttat cgaagccgac
    70561 tttttccatc tcttctttaa tacgatgtac tacgcgtttc tcgtcgcagc tttcactggg
    70621 aatagcaacc atgtcgcgca ggaagcgagt catatccgcc tggtaatctt ttgctttttc
    70681 aagaatcagt ttgaatggaa tattcttagc cattattttt ctccagattt tttactcccc
    70741 gttttgcagg gagcagttcc aatagggtga ttaaggtgct acagcgtgtt tgccttccca
    70801 gacaacttcg cggtagtgct tcacgtcggt gtcgccttca gtgctgataa ccagcaccac
    70861 ggcatctttg ttcagcgcca gtttttccat caggctttga cgttgcgggt gataatgaac
    70921 cgctgcgaga acgcccaaac cgacagcgcc ggattcaccg gagatgatgc gcgggtcgtt
    70981 gccgtacgga ttacccagca cgcgcatacc taatgcggca acgctgtcct ggcaggagat
    71041 gaattgggtg gcgcagttac gtaggatttc ccagcccagc gggttaggtt cgccacaggc
    71101 caggcctgcc atgatggtgg ccatatcacc gccaacgttg acgatgtcgc ctttgacgcc
    71161 ggagcgataa atacagtcag ctttgccagg ttcaacaata atgctgtgca gattttgcgg
    71221 gctatagacg tcgaccagat aacccagcac accaccggcc attgctccga caccggcttg
    71281 cagcagaacg tgcgtcgggg ttacgcccat ttcacgcatt tgctcgacgg cttcatctgc
    71341 cagggttgcg tagccttgca tgatccaggt tgggattttg gtgtaacctt cccatgccgt
    71401 gtcctgtacc acttcccagc cgtgctgctg cgcgtgttgc atggtcaggc gaacggtatc
    71461 gtcatagttc atatccgtga cgatgcactc ggcaccgagg ttcagaatgg cgtcaacgcg
    71521 ttcctgagca gaacctttcg gcatgtaaat caccgcattc tgtccgagtt gctgtgctgc
    71581 ccacgccaca ccgcgcccgt ggttgccgtc ggtggtggtc gcgaaagtca ttttttcgcc
    71641 gatggcattt ttcaggtgct caaatgacag cgtttcgata tcaagatgat atttttcaca
    71701 caataattga gcgatggcgt acgcaccgcc aagcattttg aaggcgttca gaccgaatcg
    71761 ttttgattcg tctttgacga gaattttctt cacaccaaaa aggtttgcga gatcgtccag
    71821 cgcacaaagc ggtgttgggc gataaccagc tattttctgg tggaactggc gcgccagttt
    71881 ggcctggctt tgcgaaaaga gcggtgatgt ttcgccgttg aaaaatttgt tatcggcgat
    71941 atcaatcttc aatgagaaaa cggacatatc ctatcctttt ggcctctgga aatagagggc
    72001 cgaccagctg atcggcccga ggggttattt cacgcgttct tgcgcttctt tcagcagttg
    72061 ctcgagcagt gcacctggtt tggcgtattt acgggacagg atcatcgcgg cgatgatata
    72121 aggcttccag ctggcttctt tgtaggtagc gatacggtat ttttcgaata cgccttcagt
    72181 cacttcacct tctttacagg atacgccgct gatatcagct ggcaggcagt gcatgtacag
    72241 ggcttcgcca tcacgggtca gttccatcat ctcttcagta caatgccagt ctttgtgttg
    72301 cgcgttctgt gccagacact gtttttccag tgcttttaag ccttcgtgat cgttcgcacg
    72361 cagcaattca gtacgctctt ccatcacttt gtaaggtgcc catgacttcg gataaacgat
    72421 gtctgcgtct ttgaaggctt cttccatgct ggtgacctga cggaagctac caccggaggc
    72481 tttagcattg tttttcgcca cttcaaccac atccgggatc aggtcgtagc cttccggatg
    72541 ggccagggtg acatccatac cgaagcgagt catcagaccg atgatgcctt gtggtacaga
    72601 gagcggtttg ccatagcttg gagagtaggc ccaggtcatg gcgatttttt tacctttcag
    72661 gttttccagt gaaccaaagt gttcacgtaa ccacgcgagg tcagccattg actgagtcgg
    72721 gtggtcaata tcgcattgca ggttcactaa agccggacgc tgtggcagta caccctgctt
    72781 gtaaccgtca tcaagtgcag cgccaacttc acgcatatat gcgttgcctg cgcccagata
    72841 catatcgtcg cgaataccaa tagcgtcggc gcagaaggag atcatattgg cggtttcacg
    72901 cacggtttcg ccgtgagcga tttgtgattt gccttcgtcg agatcttgtt gtgcaaggcc
    72961 gagcaggtta agcgcggaag cataagagaa gcgggtacgg gtggagttgt cgcggaatac
    73021 ggaaatacct aatccactat taaagacttt ggttgagatg ttttcagcac gcagtgcttt
    73081 taatgctgcg gcaacgtcca gtacttgttt cagttcatct ggcgtctgtt cccacgttaa
    73141 caaaaaatct ttctcgtgaa ggtgagaggt cagcgaattg atatccttaa tcagctcatt
    73201 aacagttttc atcataaagt cccggtagtg gaatgacagg caaagtggtt tatttcactg
    73261 tgacttgcca aagtgattgg tgttttcaca gctatacctg ggactaacaa taagtgtgcc
    73321 agaattatcc gcgcggctgc aaaagactaa tttgacggat aatgtgtggg gttgatcaca
    73381 aattgatgcc agcttctggt tgtttgtaaa atacgaaaac gtttggcttt ttttatcgcg
    73441 ttatttattt gctgctaagt atcagaaata agataaaggg gcaggctatc tctcttcagt
    73501 ttgataaact caccgccgta attctcatat tgatatagaa atgtgatatc tcttccataa
    73561 tttcattaga aaatgataaa aatcttatgt ttttatcaaa gccaatttcg cctgagttaa
    73621 tatcatcatg atctgtgaac catcaacgtc ttcaatgatc gacttttaag taagtctgga
    73681 aggtaaagct atggagcttg ctactacgca gtcagtattg atgcaaattc aaccgacaat
    73741 tcagcgtttt gccagaatgc ttgccagcgt tttgcagctt gaggttgaga tcgttgatga
    73801 aaacttgtgt cgcgttgccg gaacgggcgc gtatgggaag tttcttggtc gccagttgag
    73861 cggcaactca cgcctgctcc gccacgtcct ggaaacgaaa actgaaaaag ttgtgacaca
    73921 gtctcgcttc gatccccttt gcgaaggttg cgatagtaaa gaaaattgcc gcgaaaaagc
    73981 atttctgggt acgcctgtca ttttacagga tcgttgtgtt ggggtgataa gtttgattgc
    74041 cgttacccac gagcaacaag agcatatcag tgataattta cgcgaatttt ctgattatgt
    74101 tcgccatata tccaccattt ttgtttcgaa acttctggag gatcaggggc caggagataa
    74161 catcagtaaa atatttgcga ccatgatcga taatatggat cagggcgtat tagttgtaga
    74221 tgatgaaaat cgggttcagt ttgttaatca gactgcctta aaaacacttg gtgttgtgca
    74281 aaataatatt attgggaaac ctatccgttt cagaccatta acatttgaga gtaattttac
    74341 ccatggacat atgcagcata ttgtttcatg ggacgataaa agtgaattaa tcattggtca
    74401 attgcataac attcagggcc gacaattatt tttaatggcg tttaaccaat cgcataccag
    74461 tttttctgta gcaaatgcac ctgatgaacc gcatattgaa caattggttg gcgagtgccg
    74521 tgttatgcgg caattaaaac gactcattag ccgtattgca cccagcccat ccagcgttat
    74581 ggtggttggt gaaagcggca cgggtaaaga agtcgtcgcc cgtgcaatcc ataagttgag
    74641 cggaagacgg aataaaccct ttattgctat caactgtgcc gcgattccgg agcagcttct
    74701 ggagagcgaa ctgttcggtt atgttaaagg tgcatttact ggcgcttctg ccaacggtaa
    74761 aacagggttg attcaggcgg cgaatacggg cacgctgttt ctcgatgaaa taggtgatat
    74821 gccattaatg ttgcaggcta aattactgcg cgctattgag gcacgtgaaa ttctgccgat
    74881 tggtgccagt agcccaatac aagtcgacat tcgcatcatt tctgcaacta atcagaattt
    74941 ggcccagttc attgccgaag gtaaattccg cgaagatctc ttctaccgac ttaatgttat
    75001 cccgataact ctgccaccgc tgcgtgaacg tcaggaagat attgaactat tggtgcatta
    75061 ctttttacat ctgcataccc gtcgtctggg atcggtttat cctggcattg ctcccgatgt
    75121 cgtcgaaata ttgcgtaagc atcgttggcc cggaaacctg cgcgagttaa gcaatttgat
    75181 ggaatatctg gttaacgtgg ttccttcagg tgaagttatc gacagcacgc tattgccgcc
    75241 aaatctgctg aataatggca caacggagca aagtgatgta acagaggtca gtgaggcgca
    75301 cctgtcactc gatgatgcgg gcggcacggc gctggaggag atggaaaagc aaatgatccg
    75361 cgaggcgctt tcacgtcata acagcaagaa gcaagttgct gatgaactgg gcatcggcat
    75421 tgctacgctc tatcgcaaga ttaagaaata tgagttgtta aacacataac ctcagtgaat
    75481 ccggcctgaa ttcaggccgg atatcctttt acttcgtttt ctcgcaatcc agaactgtat
    75541 ttacaatcat ctgatacccc gtgcagcgac aaagatttcc cgccagtccg cgacgaattt
    75601 ccgtaatggt taatggcttc tcgcgtggtt tcgccagcat tgccgtggta gccataatca
    75661 ggccaggcgt acaaaacccg cactgcactg cgccggattt cgcataagcc tgctgaacat
    75721 gagaaagttt tccgcctttc gcttcacctt ccagcgtgcg gatctctttt ccttcagccc
    75781 aggcggcaag gtataagcaa ctgtctattg ctgtgccgtc gaccaacacc gtacaggcac
    75841 cacattcacc cacgcagcac ccttgtttga cacttagcag tccttgttcg cggagtaatt
    75901 ccgagagcgg cgtgcctggt gcggcgtgaa gctgaaaagg catcccgtta atggtgcatt
    75961 cgatggtaat tgtttcgctg tgattcattg caattttccc cccgccgcgg cgacggcttc
    76021 gctaatcact tttttggtca tcgtctggat gagatgcaga cgaaactctt tactggcccg
    76081 ccatgaagaa cgcggggcga catcttgcag gacagattcg ctgatagctt ccagcgtttg
    76141 caggtttaat ggcgcatttt gtgcagtctg ttcggcatgt tggcagcgaa tcggcgttgg
    76201 cgcggcaaca ccaaatgcca ggcgtaattc gctgaaattg ccgttatcca gtcggcaatg
    76261 tgcggcgcag ccaatcgttg aaatatccat tgcgtcgcgc atggcatatt taaaatgcgc
    76321 gctgcccgcg tgttctttcg gctgtggcgg aaaatgaaag gcgacgagga tttcgtcatg
    76381 ctcaagagac actttgcccg gcccggtgtg aaagccatta atcgggacga aacgaacacc
    76441 gcgcggggag tggatctcca gtttcgcgtc ataaattagc gttggcgtgg cagaatctgc
    76501 gctggtggca ccgttgcaaa tatttccacc gtaggtagcg acgttacgga tctgcggtcc
    76561 agcaatggac gtggccgcag cacataacgc cgggagatga cgttgagtta taggatcttc
    76621 tattagctgg gtaaatgtcg ttgcagagcc gatacgtagc gagccatctt ccgccagcgt
    76681 aattccccgc agctccgcca gattatgaat atcaacaata tggcgataac ggtcattgtg
    76741 atggtggagc tgaatcagta cgtcagtgcc accggcgagc agtttggcct gcgggttgtc
    76801 agccagcagg ttgatggcat cggcaagggt tgctgcgcga tggtaagaag caaaatcaaa
    76861 catgatgtta tcctcaaatc aatcctgcca gatggaactc ttcatataac cgttttggcg
    76921 tcagcggcag tgtattgatt gcaacaccgg tagccatctt caccgcgtta cgaatagcag
    76981 cggcaacagg aattattggt ggctcaccca gtgacttatg tccgtatgcg gattgcggct
    77041 cattgatttc gacgaacgcg ctttccagtt gtggcagatc cggcatggtc ggcattttgt
    77101 aatccagcag attggggtta cggaccacgc cgcttttagc atcgatgatc atctcttcaa
    77161 atagcgccca gccaatgccc attcccattc cgccgtgtac ctgaccttct gccagcagtg
    77221 gattaagaat atgccctgaa tcatgaacgt tgaggatgcg gttgatggtg actttgcaca
    77281 gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa cgccggtggg ttagtggtgg
    77341 ttttgatgga gctttcagca gagagctgcc cgccgcgttc agggtggtag aaagcgtcca
    77401 tcgccaaatc ttttaacgac attaacggct cttccggtcg ttcaaccagc acgatatggc
    77461 cttttatcag ggtcagattc atcgctgact gatgtagcat gactgcggcg tgagcgatga
    77521 ttttctcttt taataatagt gccgcactgc gcagcgcagg cgcggcaaca tagctctggc
    77581 gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt atcttgagtt gaaataacgc
    77641 gaacgtcgct gaccggaacc cccacggttt ctgccaccat ttgcgagaag acggtgtcgg
    77701 caccctgacc gatttccgtc gcgccgcttt gcacgttgat ggttccatcc tgattcatca
    77761 gaaggcgcgc gcctgctatt tctacgccga caggccaggt gttagaggtg tagctaaaac
    77821 aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt ctggcattct gcacggcgtt
    77881 tttcccattc aaagattttc cggccttttt caagacactc cggcaaccct gcgctgtaaa
    77941 tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc ggcgttgcgt aaacgaattt
    78001 caacaggatc aatacctaac gctgtcgcgg cgtcatcaag catagactca acggcaaata
    78061 cgacttgtgg cgcgccataa ccacgcatcg caccagccga ggggaggttg gtatagcagg
    78121 tctttgaact gtaagcgtag gcacaacgag gataaaggta agcgacttta ttcccccccg
    78181 cagaagcgat ggagtgcccg tgagatgcat aagcgccggt gttagacaga acatccagac
    78241 tataaccttt caatgttccg tcgcggttca cgcccatttg cccgtcaatg gtaaaagcgt
    78301 ggcgggtacg ggttgcgagg aaacactctt cacggctaag ggaaactttc accggaatgc
    78361 cgccaagctt gctggtcagg aatgccgcca ttggctcttc cagtacatcc tgtttattac
    78421 caaaaccgcc accgacaaat ggtttgatga ctcgtacgca tgaccaggga atatccagcg
    78481 cctgaccaac cacgcggcga acaatgtgcg ggatctgggt gctggaaacg atggtaattc
    78541 gcgagtcatc ctccatccac gccagcgatg ttacgctttc catgtgacaa tgttgaataa
    78601 cgggggtctg atagtgcccc tgtacctggt agtcggcggc atcgattgtt tgttggacat
    78661 tacccgtcga catcgtgctt tgtttcagta aattgccacc gttatggatt ggtgcagcgt
    78721 cttctgccag cgccgcttct ggcgtggtga taacgggtaa ttcttgccac tcaatgctga
    78781 ccaattgcgc cgctttttct gccgtgagtt catcgtgggc cacgacgatg gcaacggcgt
    78841 caccatgatg acgaacatgg cgagttagca gtgcgcgatc ggcggtatcg cgcttgtttt
    78901 cgtcaagtgt ccaggcatgc ccagctgtag cgaatggaat atcaggcaca tcttcccagg
    78961 taaaaatcgc cagtacgccc ggcaaacttc tggcttgttc atcattaata cttacggcat
    79021 aaccatgtgc gatagggcta cgtacatatt tcgcataaca catgcccgcc ataacataat
    79081 cgtcagtata tcgtgcccgc ccggtgacct tagcaatggc atcgacgcgc atgcatgatt
    79141 cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc ctcgatttga acatttggtt
    79201 cttatggcaa agatttagtc taagcaggca agatttacgc cagaaacgac gataagcgtt
    79261 aggggaggta ggaagtgtga tgacgttaaa aaaacacaga tatgatacga aacaggactc
    79321 tgaatagggg cagtgatacg tatcaatatg agtggcgata aaatcaatgt gataaattgg
    79381 gattattacg catcaggatg ggaaataggg taaaaagtca ggaagttcga tacctctaca
    79441 gcgaatattt tgctgatgat acttcccttt gccaaatgaa attaacctct ttctctatta
    79501 ccacgttttt ccagaagcaa gagattgggt atcaagaggc tggctgctat gataaggcgt
    79561 cttgtttttt aagcgaggaa agattttgag tgcgggacgc ctgaataaaa aatctctggg
    79621 tatcgtgatg ttgttatcgg ttggactgct tttggcgggc tgttcgggta gcaaatcatc
    79681 cgatacagga acgtattccg gctccgttta caccgtgaaa cggggggata cgctatatcg
    79741 tatttcgcgc accacgggaa ccagcgtaaa agaactggcg cgactgaacg gcatttcccc
    79801 cccttacacc attgaagttg gtcagaaact aaaactgggt ggggcgaaaa gtagcagtat
    79861 tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca tcggttacac cgtcatcagc
    79921 ggtaccgaaa tcatcctggc cgccagtagg gcaacgttgt tggttatggc caacgacagg
    79981 gaaagttatc atgccgtatt cgacagcaga tggcggcaat aaagggattg atatctcagc
    80041 tccacggggt acacctattt acgccgcggg tgcaggaaag gtggtgtatg tgggcaacca
    80101 gctgcgtggc tacggtaatc tcatcatgat taaacacagt gaagattaca ttacggctta
    80161 cgcccataat gacacgatgc tggtaaataa tgggcaaagc gtgaaggctg ggcaaaaaat
    80221 cgccactatg gggagcacgg atgcggcatc tgttcgcctg catttccaga ttcgttaccg
    80281 tgcaacggca attgatccgc tacgttactt gccgcctcag ggcagcaagc caaaatgctg
    80341 atggcgaatt aatcagcagt cagcagcgtg gcacttgcta aggagagcgt aaggtttata
    80401 atgccttacg catctcgaag cgggcgtagt tcaatggtag aacgagagct tcccaagctc
    80461 tatacgaggg ttcgattccc ttcgcccgct ccaatttatc ttcgccgtaa tacttcccca
    80521 ttttgttgta aataaaaagc gtttacgcaa tgcaatcctt ttgtaacaac ccaggtaaaa
    80581 caccttattt tcaagttgtt aaataagtgg ttatctacgc ttttaacaaa cgattgatgg
    80641 aatattttat gaaagggaaa tctgcattaa ctcttctgct cgctggtata ttttcgtgcg
    80701 gcacatgcca ggcgacaggg gcagaagtga cgagtgaatc tgtatttaac attcttaatt
    80761 ccacgggggc agcaacagat aaaagttatt tatcgttaaa tccagataag tatcctaatt
    80821 atcgtcttct gattcactca gccaaattac aaaatgaaat aaagagccac tataccaaag
    80881 acgaaattca aggattgcta acattaacag aaaacacacg aaagttaacg ttgacggaga
    80941 aaccctgggg aacgtttatt cttgcttcta ctttcgaaga tgacaaaaca gcggcagaaa
    81001 cccactatga tgctgtctgg ttgagggata gtttatgggg ctatatggca ttagtatcag
    81061 atcagggaaa tagtgtagcc gcaaaaaagg ttctgctcac cctgtgggat tatatgtcta
    81121 cgcccgacca gattaagcgg atgcaggacg tcattagcaa cccaaaacga ctggatggaa
    81181 ttcccgtgca aatgaatgct gtgcatatcc gatttgacag caactccccc gtgatggcag
    81241 atgtgcagga agaaggcaag ccgcagctgt ggaaccataa acaaaatgat gctttaggtc
    81301 tttaccttga tctccttata caagcaatca acactggtac tattaacgct gaagattggc
    81361 aaaaaggtga ccgattaaag tcggttgcgc tgctgattgc ttatctggat aaagccaatt
    81421 tctatgttat ggaagattcc tgagcatggg aagaagatgc ccgattaaac acatcttccg
    81481 ttgcactggt gacctctgga ctggaacgtt tgtcaaacct actctcgaag aaagactctg
    81541 tttttgtctc tgatttacta cgcgaagcta aagtcaatga actggatgaa actctttcaa
    81601 caacgaggtt gaaccacctc atagataaag gttatgaaag aataacgtta cagcttgatt
    81661 tagggggaga atccccaggt tatctggaaa aagataaaca ttacagagaa gccgatgcgg
    81721 ctttacttaa tgttatttat cctacaaatc tttcaaagat aaacaccaga agaaaagagc
    81781 aagttctaaa aatagttaaa aaactagcag gtccgtatgg tataaaaaga tacgagaagg
    81841 ataattatca gtcggcaaat ttttggttca atgatattaa aaccgatacc gatcaaaatt
    81901 cgcatgcaaa aagagaaaag agcttcattc catcaacaga agcagaatgg ttttttgact
    81961 catggtatgc aaaatcagca gcaattgttt ataaagaatc aagaaaagaa gagtatttga
    82021 atgactcagt acaatttatg aatagatcat tggcccaaat aacaggcgag aatatgattg
    82081 gtgcaaatgg caggagcgta ccagaaatgg cacttcccga aagctataac tatatccaca
    82141 agtcgggaac tttgcatgaa gcgccaagcc ctatcattcc gctaaattgg tcgaaagctt
    82201 caatgactct catgttgaaa gagatgtcca accttatcaa tgatgaggga attaaatagt
    82261 tttcataatg agttacagca caacgttggg tattgactga gcaagatgaa cgacaaggac
    82321 aacgacatca gccttatgag gagcaagaaa atgaaagcaa attcgaaaac gatcagaagg
    82381 atgaaagtta atgttcggtc tgaggaaagt aaatcgaaac actcatacgt ttgaaaccac
    82441 ttttcaaaat tggaaagaaa atggcgaaga tgttgcatta ttaatgcccg aatttagtgc
    82501 taaatggcta ccgatagcag aagagagtgg cagctggtct ggatgggtat tgcttggaga
    82561 aatatttccc ttaatttcat ctgagctcgc tgggatggct ttaatgccag aaacggaaag
    82621 gttaataggg gaatgcctca gtttatccag ttccccgtta aatctaaagc atcctgaact
    82681 aaaatataat cgtttgcgcg taggtaaagt atttgatgga gtattgagcc ctgctcaacc
    82741 actaataaga atatggacag gggaattgaa tctttggtta gataaagtca cagtctgcca
    82801 atacggaaac gctccagcgt tagacaaaaa atcgttatat tgctccattc attttgtaat
    82861 tggatttagt aaaacatgtt acagaagtct tgttgatatt gaagttggtg atgttttatt
    82921 aatctcaaat aatttggctt atgcggttat ttataataca aaaatttgtg atttaattta
    82981 tccagaggag ttaaaaatgg ctgatcattt tgagtacgag gaagattttg aaacagatga
    83041 ttttgatatc aaaaaaaacg agagtgagat ttatgatgaa aatgacgatc agatgattaa
    83101 tagttttgaa gacttgcctg taaaaattga gtttgttctt ggtaagaaaa taatgaatct
    83161 atatgaaata gacgaactct gtgcaaaaag aataatatct ctgctttctg aatctgagaa
    83221 aaatatagaa atacgtgtaa atggcgcgct aactggctat ggcgaacttg ttgaagtgga
    83281 tgataaattg ggcgtagaga ttcattcttg gttatcaggg cataataatg tcaaatagca
    83341 tttcgctgat agctatacta tcattattta cactattgcc gtttataata gcatccggga
    83401 catattttat taaattctcg attgtttttg ttattgtccg aaacgcgctg gggcttcagc
    83461 aagtgccatc aaatatgaca cttaatgggg ttgctttgct attgtcaatg tttgtaatga
    83521 tgccggttgg gaaggaaata tattataaca gtcagaatga gaatctttca ttcaataatg
    83581 tagcgtcggt tgttaatttt gttgagacag gcatgagtgg ttataaatca tatttgataa
    83641 aatattccga accggagttg gttaactttt ttgaaaagat tcagaaggtg aatagctctg
    83701 aagacaatga agagatattc gatgatgata atatttcaat tttctcgtta ctcccggcat
    83761 atgcgttgag tgagataaaa agtgcattta ttataggttt ttatatctat ttaccctttg
    83821 ttgtagtgga tctggttata tccagcgttc tgttaacgtt aggtatgatg atgatgagtc
    83881 cggtaaccat atctacaccg ataaaattaa ttttgtttgt cgcgatggac ggttggacaa
    83941 tgttatcaaa aggactcatt ttacaatatt ttgacctatc cattaatccc tgaggaaaaa
    84001 atatggacga tattgttttt gcagggaata gggcgctgta tctgatttta gttatgtctg
    84061 caggacctat cgcagttgcg acatttgttg gtttattagt agggcttttt caaacggtaa
    84121 cacagttgca agaacagacg ttgccatttg gcgtgaaatt attatgcgta agtatttgct
    84181 tttttttaat gagcggatgg tatggcgaaa aattatattc ttttggtatt gaaatgctga
    84241 atttagcatt cgcgagagga tagcatgggt gaggcgatac tctatcagtt gcatagtctg
    84301 ctggctgcta cagcgctctg tttttgccga ttggcaccca ctttctattt gttaccattt
    84361 ttcgccagtg ggaatatacc aacagtagtc cgtcatccaa taataatagt ggtatcatgt
    84421 gcattagttc agcattacca ctatgaatta ttgaacttaa atgaaatcga tattgctttg
    84481 ttcgctgcca gagaaattat catagggctg tttattgcat gcttattagc atccccgttt
    84541 tggattttcc ttgctatagg tagctttatc gataaccaac gcggtgcaac attaagtagt
    84601 actttggatc cggctacagg tgtcgatacc tcggaattag caagattatt taaccttttc
    84661 tctgctgcgg tttatcttac taaaggtgga atgaacttta ttctggaaac tctttg
//
LOCUS       DBBGLQ010000010        82675 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.010, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000010 DBBGLQ010000000
VERSION     DBBGLQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.010"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            36..203
                     /gene="yhfL"
                     /locus_tag="ACIYSB_000938"
     CDS             36..203
                     /gene="yhfL"
                     /locus_tag="ACIYSB_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGC5428032.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            498..1835
                     /gene="frlA"
                     /locus_tag="ACIYSB_000939"
     CDS             498..1835
                     /gene="frlA"
                     /locus_tag="ACIYSB_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGC5428033.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1856..2878
                     /gene="frlB"
                     /locus_tag="ACIYSB_000940"
     CDS             1856..2878
                     /gene="frlB"
                     /locus_tag="ACIYSB_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGC5428034.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2928..3758
                     /gene="frlC"
                     /locus_tag="ACIYSB_000941"
     CDS             2928..3758
                     /gene="frlC"
                     /locus_tag="ACIYSB_000941"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGC5428035.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3755..4540
                     /gene="frlD"
                     /locus_tag="ACIYSB_000942"
     CDS             3755..4540
                     /gene="frlD"
                     /locus_tag="ACIYSB_000942"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HGC5428036.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAIAQGTACAAKTIQYHGAW"
     gene            4640..5371
                     /gene="frlR"
                     /locus_tag="ACIYSB_000943"
                     /gene_synonym="yhfR"
     CDS             4640..5371
                     /gene="frlR"
                     /locus_tag="ACIYSB_000943"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGC5428037.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5523..6608)
                     /gene="yhfS"
                     /locus_tag="ACIYSB_000944"
     CDS             complement(5523..6608)
                     /gene="yhfS"
                     /locus_tag="ACIYSB_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGC5428038.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6620..7924)
                     /gene="yhfT"
                     /locus_tag="ACIYSB_000945"
     CDS             complement(6620..7924)
                     /gene="yhfT"
                     /locus_tag="ACIYSB_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HGC5428039.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7936..8289)
                     /gene="yhfU"
                     /locus_tag="ACIYSB_000946"
     CDS             complement(7936..8289)
                     /gene="yhfU"
                     /locus_tag="ACIYSB_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HGC5428040.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8300..9178)
                     /gene="php"
                     /locus_tag="ACIYSB_000947"
     CDS             complement(8300..9178)
                     /gene="php"
                     /locus_tag="ACIYSB_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HGC5428041.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9175..10401)
                     /gene="yhfW"
                     /locus_tag="ACIYSB_000948"
     CDS             complement(9175..10401)
                     /gene="yhfW"
                     /locus_tag="ACIYSB_000948"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGC5428042.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10401..11564)
                     /gene="yhfX"
                     /locus_tag="ACIYSB_000949"
     CDS             complement(10401..11564)
                     /gene="yhfX"
                     /locus_tag="ACIYSB_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HGC5428043.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFALKVLPEIVAEIQNLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(11648..12009)
                     /gene="yhfY"
                     /locus_tag="ACIYSB_000950"
                     /pseudo
     CDS             complement(11648..12009)
                     /gene="yhfY"
                     /locus_tag="ACIYSB_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(12026..12931)
                     /gene="yhfZ"
                     /locus_tag="ACIYSB_000951"
     CDS             complement(12026..12931)
                     /gene="yhfZ"
                     /locus_tag="ACIYSB_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HGC5428044.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELANECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13221..14225)
                     /gene="trpS"
                     /locus_tag="ACIYSB_000952"
     CDS             complement(13221..14225)
                     /gene="trpS"
                     /locus_tag="ACIYSB_000952"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGC5428045.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14218..14976)
                     /gene="gph"
                     /locus_tag="ACIYSB_000953"
     CDS             complement(14218..14976)
                     /gene="gph"
                     /locus_tag="ACIYSB_000953"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGC5428046.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14969..15646)
                     /gene="rpe"
                     /locus_tag="ACIYSB_000954"
     CDS             complement(14969..15646)
                     /gene="rpe"
                     /locus_tag="ACIYSB_000954"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGC5428047.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15664..16500)
                     /gene="dam"
                     /locus_tag="ACIYSB_000955"
     CDS             complement(15664..16500)
                     /gene="dam"
                     /locus_tag="ACIYSB_000955"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGC5428048.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16607..17893)
                     /gene="damX"
                     /locus_tag="ACIYSB_000956"
     CDS             complement(16607..17893)
                     /gene="damX"
                     /locus_tag="ACIYSB_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGC5428049.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGAKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17985..19073)
                     /gene="aroB"
                     /locus_tag="ACIYSB_000957"
     CDS             complement(17985..19073)
                     /gene="aroB"
                     /locus_tag="ACIYSB_000957"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGC5428050.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19130..19651)
                     /gene="aroK"
                     /locus_tag="ACIYSB_000958"
     CDS             complement(19130..19651)
                     /gene="aroK"
                     /locus_tag="ACIYSB_000958"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGC5428051.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(20052..21290)
                     /gene="hofQ"
                     /locus_tag="ACIYSB_000959"
     CDS             complement(20052..21290)
                     /gene="hofQ"
                     /locus_tag="ACIYSB_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGC5428052.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21202..21606)
                     /gene="hofP"
                     /locus_tag="ACIYSB_000960"
     CDS             complement(21202..21606)
                     /gene="hofP"
                     /locus_tag="ACIYSB_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGC5428053.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21596..22036)
                     /gene="hofO"
                     /locus_tag="ACIYSB_000961"
     CDS             complement(21596..22036)
                     /gene="hofO"
                     /locus_tag="ACIYSB_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGC5428054.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(22020..22559)
                     /gene="hofN"
                     /locus_tag="ACIYSB_000962"
     CDS             complement(22020..22559)
                     /gene="hofN"
                     /locus_tag="ACIYSB_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGC5428055.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22559..23338)
                     /gene="hofM"
                     /locus_tag="ACIYSB_000963"
     CDS             complement(22559..23338)
                     /gene="hofM"
                     /locus_tag="ACIYSB_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGC5428056.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23458..26010
                     /gene="mrcA"
                     /locus_tag="ACIYSB_000964"
     CDS             23458..26010
                     /gene="mrcA"
                     /locus_tag="ACIYSB_000964"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGC5428057.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDASAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26176..26736)
                     /gene="nudE"
                     /locus_tag="ACIYSB_000965"
     CDS             complement(26176..26736)
                     /gene="nudE"
                     /locus_tag="ACIYSB_000965"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGC5428058.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGKGRV"
     gene            27056..29191
                     /gene="igaA"
                     /locus_tag="ACIYSB_000966"
     CDS             27056..29191
                     /gene="igaA"
                     /locus_tag="ACIYSB_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGC5428059.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     SYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29256..29924
                     /gene="yrfG"
                     /locus_tag="ACIYSB_000967"
     CDS             29256..29924
                     /gene="yrfG"
                     /locus_tag="ACIYSB_000967"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGC5428060.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29935..30336
                     /gene="hslR"
                     /locus_tag="ACIYSB_000968"
     CDS             29935..30336
                     /gene="hslR"
                     /locus_tag="ACIYSB_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGC5428061.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30361..31239
                     /gene="hslO"
                     /locus_tag="ACIYSB_000969"
     CDS             30361..31239
                     /gene="hslO"
                     /locus_tag="ACIYSB_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGC5428062.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31302..33026)
                     /gene="yhgE"
                     /locus_tag="ACIYSB_000970"
     CDS             complement(31302..33026)
                     /gene="yhgE"
                     /locus_tag="ACIYSB_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGC5428063.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQVSEKV
                     LADNVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLNVNLNE"
     gene            33405..35027
                     /gene="pckA"
                     /locus_tag="ACIYSB_000971"
     CDS             33405..35027
                     /gene="pckA"
                     /locus_tag="ACIYSB_000971"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGC5428064.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35103..36455)
                     /gene="envZ"
                     /locus_tag="ACIYSB_000972"
     CDS             complement(35103..36455)
                     /gene="envZ"
                     /locus_tag="ACIYSB_000972"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGC5428065.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36452..37171)
                     /gene="ompR"
                     /locus_tag="ACIYSB_000973"
     CDS             complement(36452..37171)
                     /gene="ompR"
                     /locus_tag="ACIYSB_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGC5428066.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37399..37875
                     /gene="greB"
                     /locus_tag="ACIYSB_000974"
     CDS             37399..37875
                     /gene="greB"
                     /locus_tag="ACIYSB_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGC5428067.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37972..40293
                     /gene="yhgF"
                     /locus_tag="ACIYSB_000975"
     CDS             37972..40293
                     /gene="yhgF"
                     /locus_tag="ACIYSB_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGC5428068.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRSAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40750..40977
                     /gene="feoA"
                     /locus_tag="ACIYSB_000976"
     CDS             40750..40977
                     /gene="feoA"
                     /locus_tag="ACIYSB_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGC5428069.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40994..43315
                     /gene="feoB"
                     /locus_tag="ACIYSB_000977"
     CDS             40994..43315
                     /gene="feoB"
                     /locus_tag="ACIYSB_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGC5428070.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43315..43551
                     /gene="feoC"
                     /locus_tag="ACIYSB_000978"
     CDS             43315..43551
                     /gene="feoC"
                     /locus_tag="ACIYSB_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGC5428071.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43754..44632
                     /gene="rpnA"
                     /locus_tag="ACIYSB_000979"
     CDS             43754..44632
                     /gene="rpnA"
                     /locus_tag="ACIYSB_000979"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGC5428072.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(44661..45431)
                     /gene="bioH"
                     /locus_tag="ACIYSB_000980"
     CDS             complement(44661..45431)
                     /gene="bioH"
                     /locus_tag="ACIYSB_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGC5428073.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCHLLVALKQRV"
     gene            45469..46152
                     /gene="gntX"
                     /locus_tag="ACIYSB_000981"
     CDS             45469..46152
                     /gene="gntX"
                     /locus_tag="ACIYSB_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGC5428074.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIVSVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAAAV
                     QVWCLCRTL"
     gene            46211..46786
                     /gene="nfuA"
                     /locus_tag="ACIYSB_000982"
     CDS             46211..46786
                     /gene="nfuA"
                     /locus_tag="ACIYSB_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGC5428075.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47146..48462
                     /gene="gntT"
                     /locus_tag="ACIYSB_000983"
     CDS             47146..48462
                     /gene="gntT"
                     /locus_tag="ACIYSB_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGC5428076.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48573..50657)
                     /gene="malQ"
                     /locus_tag="ACIYSB_000984"
     CDS             complement(48573..50657)
                     /gene="malQ"
                     /locus_tag="ACIYSB_000984"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGC5428077.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50667..53060)
                     /gene="malP"
                     /locus_tag="ACIYSB_000985"
     CDS             complement(50667..53060)
                     /gene="malP"
                     /locus_tag="ACIYSB_000985"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGC5428078.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSVAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53672..56377
                     /gene="malT"
                     /locus_tag="ACIYSB_000986"
     CDS             53672..56377
                     /gene="malT"
                     /locus_tag="ACIYSB_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGC5428079.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQDAVQHAQQLLKMMGYGV"
     gene            complement(56420..57436)
                     /gene="rtcA"
                     /locus_tag="ACIYSB_000987"
     CDS             complement(56420..57436)
                     /gene="rtcA"
                     /locus_tag="ACIYSB_000987"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGC5428080.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     TAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTR
                     VSIE"
     gene            complement(57440..58666)
                     /gene="rtcB"
                     /locus_tag="ACIYSB_000988"
     CDS             complement(57440..58666)
                     /gene="rtcB"
                     /locus_tag="ACIYSB_000988"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGC5428081.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEISMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            58855..60453
                     /gene="rtcR"
                     /locus_tag="ACIYSB_000989"
     CDS             58855..60453
                     /gene="rtcR"
                     /locus_tag="ACIYSB_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGC5428082.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(60435..61193)
                     /locus_tag="ACIYSB_000990"
     CDS             complement(60435..61193)
                     /locus_tag="ACIYSB_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGC5428083.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(61210..62040)
                     /gene="glpG"
                     /locus_tag="ACIYSB_000991"
     CDS             complement(61210..62040)
                     /gene="glpG"
                     /locus_tag="ACIYSB_000991"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGC5428084.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(62085..62411)
                     /gene="glpE"
                     /locus_tag="ACIYSB_000992"
     CDS             complement(62085..62411)
                     /gene="glpE"
                     /locus_tag="ACIYSB_000992"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGC5428085.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            62601..64106
                     /gene="glpD"
                     /locus_tag="ACIYSB_000993"
     CDS             62601..64106
                     /gene="glpD"
                     /locus_tag="ACIYSB_000993"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGC5428086.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS"
     gene            complement(64375..64596)
                     /gene="yzgL"
                     /locus_tag="ACIYSB_000994"
                     /pseudo
     CDS             complement(64375..64596)
                     /gene="yzgL"
                     /locus_tag="ACIYSB_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YzgL"
     gene            complement(64722..67169)
                     /gene="glgP"
                     /locus_tag="ACIYSB_000995"
     CDS             complement(64722..67169)
                     /gene="glgP"
                     /locus_tag="ACIYSB_000995"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGC5428087.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(67188..68621)
                     /gene="glgA"
                     /locus_tag="ACIYSB_000996"
     CDS             complement(67188..68621)
                     /gene="glgA"
                     /locus_tag="ACIYSB_000996"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGC5428088.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(68621..69916)
                     /gene="glgC"
                     /locus_tag="ACIYSB_000997"
     CDS             complement(68621..69916)
                     /gene="glgC"
                     /locus_tag="ACIYSB_000997"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGC5428089.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(69934..71907)
                     /gene="glgX"
                     /locus_tag="ACIYSB_000998"
     CDS             complement(69934..71907)
                     /gene="glgX"
                     /locus_tag="ACIYSB_000998"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGC5428090.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(71904..74090)
                     /gene="glgB"
                     /locus_tag="ACIYSB_000999"
     CDS             complement(71904..74090)
                     /gene="glgB"
                     /locus_tag="ACIYSB_000999"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGC5428091.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(74363..75466)
                     /gene="asd"
                     /locus_tag="ACIYSB_001000"
     CDS             complement(74363..75466)
                     /gene="asd"
                     /locus_tag="ACIYSB_001000"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGC5428092.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            75659..76252
                     /gene="yhgN"
                     /locus_tag="ACIYSB_001001"
     CDS             75659..76252
                     /gene="yhgN"
                     /locus_tag="ACIYSB_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGC5428093.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(76309..77649)
                     /gene="gntU"
                     /locus_tag="ACIYSB_001002"
     CDS             complement(76309..77649)
                     /gene="gntU"
                     /locus_tag="ACIYSB_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGC5428094.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(77653..78180)
                     /gene="gntK"
                     /locus_tag="ACIYSB_001003"
     CDS             complement(77653..78180)
                     /gene="gntK"
                     /locus_tag="ACIYSB_001003"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGC5428095.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(78319..79314)
                     /gene="gntR"
                     /locus_tag="ACIYSB_001004"
     CDS             complement(78319..79314)
                     /gene="gntR"
                     /locus_tag="ACIYSB_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGC5428096.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(79538..80233)
                     /gene="yhhW"
                     /locus_tag="ACIYSB_001005"
     CDS             complement(79538..80233)
                     /gene="yhhW"
                     /locus_tag="ACIYSB_001005"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGC5428097.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(80356..81393)
                     /gene="yhhX"
                     /locus_tag="ACIYSB_001006"
     CDS             complement(80356..81393)
                     /gene="yhhX"
                     /locus_tag="ACIYSB_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGC5428098.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            81726..82214
                     /gene="yhhY"
                     /locus_tag="ACIYSB_001007"
     CDS             81726..82214
                     /gene="yhhY"
                     /locus_tag="ACIYSB_001007"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGC5428099.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            82451..>82675
                     /gene="tssD"
                     /locus_tag="ACIYSB_001008"
     CDS             82451..>82675
                     /gene="tssD"
                     /locus_tag="ACIYSB_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HGC5428100.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFIN"
BASE COUNT        19804 a        22610 c        20890 g        19371 t
ORIGIN      
        1 aatattaatt aatatataat acatagggaa tgtaaatgaa caaatttatt aaagttgcac
       61 tggtaggtgc agtactggct acgttaactg catgtactgg tcatattgaa aaccgtgata
      121 agaactgctc ttacgactac ctgctgcacc cggcaatttc tatttctaaa atcattggcg
      181 gttgcggtcc tactgcacag taagtctctg acagaaatcg gctaacaccg gtttaattgc
      241 ccgacgccct ctggattttc cgagggcgta tttgtttcta tgtccttctt gcccccgccc
      301 gtaaaaatat tttcacttta aattcaattt gataactaca tccattagcc acagcgcgat
      361 cctgtgcgaa attttgtgat cttcctccac attacataac atcatatgtt gttatattca
      421 tcatgcattg tcatgttacc ttttaaatga ctgcaaactc tcccctacaa cgtattggac
      481 aagaaaaagg tatcgctatg ggaagccagg aactccaacg caagctcgga ttttgggccg
      541 ttcttgcaat cgccgtcggg acaaccgtcg gctccggtat ttttgtatct gtgggtgaag
      601 tggcaaaagc agcgggcacg ccgtggctta cggtgctcgc gtttgtcatt ggcgggttaa
      661 ttgtgatccc gcaaatgtgc gtctatgcgg aactatccac cgcttatccg gaaaatggcg
      721 cagattatgt ttatctgaaa aatgccggaa gccgaccgct ggctttcctc tccggctggg
      781 ccagcttctg ggccaacgat gcgccgtcat tgtcgattat ggcgctggcg attgtcagca
      841 atcttggctt tttaacgcct atcgatccgt tgctcggtaa atttatcgcc gccggattaa
      901 ttatcgcctt tatgttgcta cacctgcgct ccgttgaagg cggcgcagcg tttcagacgc
      961 taattaccat cgccaaaatt atcccgttca ctatcgtcat tggccttggg atcttctggt
     1021 ttaaagcgga gaattttgcc gcccctacca ccactgcgat tggcgcaacg ggcagcttta
     1081 tggcgctgct ggcggggatc tctgccacca gttggtcgta taccggcatg gcctctatct
     1141 gttatatgac cggcgaaatt aaaaaccccg gaaaaaccat gccacgagcg ctgattggtt
     1201 cctgtctgct ggttctggtg ctctacaccc tgctggcgct ggtgatttct ggcctgatgc
     1261 ccttcgacaa actcgccaat tctgaaacgc cgatttccga cgccctgacc tggatccccg
     1321 cactcggcag caccgctggg atctttgttg ccatcacggc gatgatcgtc attcttggtt
     1381 cgctttccag ctgcgtgatg taccagccgc ggctggaata cgcgatggcg aaagacaacc
     1441 tgttctttaa atgcttcggc catgtgcatc cgaaatacaa cacgccggat gtctccatca
     1501 tcctgcaagg ggcgctgggg atcttcttca tcttcgtttc cgatctcacc agcctgctgg
     1561 gttatttcac cctggtgatg tgtttcaaaa ataccctcac cttcggctcc atcatctggt
     1621 gtcgtaaacg cgacgattac aaaccgctgt ggcgtactcc ggctttcggg ctgatgacca
     1681 ccctcgccat tgcgtcaagc ctcattctgg tcgcctcaac ctttgtctgg gcaccgattc
     1741 ccggccttat ctgcgccgtc atcgttattg ctactggtct gcctgcttac gccttctggg
     1801 cgaagcgtag ccgccagctc aacgctttgt cgtaatgtta tctggagaaa ataaaatgtt
     1861 ggatattgat aaaagcaccg tggactttct ggtcaccgaa aatatggttc aggaagtgga
     1921 aaaagttctc agccatgacg ttccgctggt gcacgccatc gtggaagaga tggtgaagcg
     1981 cgacattgat cgtatttatt tcgttgcctg cggatcgcca ctcaacgcgg cgcaaacggc
     2041 gaaacatctg gcggatcgct tttccgatct tcaggtctac gccatttccg gctgggagtt
     2101 ctgcgataac accccgtatc gcctcgacga tcgttgcgca gtaattggcg tttctgacta
     2161 cggtaaaacc gaagaggtaa tcaaagcgct ggagctgggc cgggcctgcg gcgcactcac
     2221 tgcggcgttc accaaacgcg cggatagccc gattacctcg gcggcggaat ttagcattga
     2281 ttatcaggcc gactgtatct gggaaattca cctgctgctc tgctacagcg tggtgctgga
     2341 gatgatcacc cgcctcgcgc cgaacgcgga aatcggcaag atcaaaaacg atctcaagca
     2401 gttgccgaat gcgctcggtc atctggtacg cacctgggaa gaaaaaggcc gccagcttgg
     2461 tgaactggcc agccagtggc cgatgattta taccgttgct gcgggtccgc tgcgtccgct
     2521 gggttacaaa gaaggcattg taacgctgat ggaatttacc tggacgcacg gctgcgtgat
     2581 tgagagcgga gagttccgcc atggcccgct ggagattgtc gaaccgggcg ttccgttcct
     2641 gttcctgctc ggcaatgatg aaagtcgcca caccaccgaa cgcgccatta actttgttaa
     2701 acagcgtact gacaacgtga tcgtcatcga ttacgccgaa atttcgcaag ggctgcaccc
     2761 gtggctggca ccgttcctga tgttcgtgcc aatggagtgg ctctgctact acctgtctat
     2821 ttacaaagat cacaacccgg atgaacgccg ctattacggt ggtctggtgg aatattaatc
     2881 cctctcccgg cccggcaacg ggccggattt acgcaaggag ttacccgatg aaaacaggta
     2941 tgtttacctg cggccaccag cggctgccta ttgaacacgc atttcgtgat gcaagcgagc
     3001 tgggttacga cggcatcgaa atttggggcg gtcgcccgca cgcgttcgcg ccggacttaa
     3061 aagcgggcgg catcaaacaa atcaaggcgc tggcgcagac gtatcagatg ccgattatcg
     3121 gctatacgcc agaaaccaac ggctatccgt ataacatgat gctgggcgat gaacatatgc
     3181 gtcgcgaaag cctcgacatg atcaagctgg cgatggatat ggcaaaagag atgaacgcgg
     3241 gttatacgct gatttccgcg gcccacgcgg gctatctcac gccacctaat gttatctggg
     3301 gacggctggc agagaaccta agcgaactgt gtgagtacgc ggaaaacatc ggcatggatc
     3361 taatcctcga accgttaacg ccgtatgaat cgaacgtcgt gtgtaatgct aatgatgtgc
     3421 ttcatgcgct ggcgctggtg ccttcgccgc gcctgttcag catggtcgac atttgcgcgc
     3481 cgtatgtcca ggcggaaccg gtgatgagtt atttcgacaa actgggcgat aaattacgtc
     3541 atctgcatat tgtcgacagc gacggggcca gcgacacgca ttacattcct ggagaaggca
     3601 aaatgccgct gcgggaactg atgcgcgata ttattgagcg gggctatgag ggttactgta
     3661 cggtggagct ggtgacgatg tatatgaacg agcccagact ctatgcccgc caggcgctgg
     3721 aacgctttcg cgcgctgctg ccggaggatg agagatgaaa accctggcga caatcggcga
     3781 taactgcgtc gatatctacc cgcaactgaa taaagcgttt tctggcggta atgcggtcaa
     3841 tgtggcggtg tactgcactc gctacggcat acagccggga tgcattacct gggtgggtga
     3901 cgatgactac ggcacaaagc tgaagcagga tctcgcccgc atgggcgtcg atatcagcca
     3961 tgtccatacg aaacacggcg ttaccgcaca aactcaggtg gaactgcacg acaatgatcg
     4021 cgtttttggc gactacaccg aaggcgtgat ggccgacttt gccctgagtg aagaggatta
     4081 cgcctggctg gcgcagtatg acattgtgca cgcggcaatc tggggacatg cggaagacgc
     4141 attcccacag ctgcacgctg cgggcaaact taccgctttc gacttctccg acaagtggga
     4201 cagcccgctc tggcagacac tggtgccgca tctcgatttt gcctttgcct ccgcaccgca
     4261 agaagacgaa acgctgcgtc tgaagatgaa agcgattgtt gcccgtggcg caggaacagt
     4321 gattgtcacg ctgggtgaaa acggcagcat tgcctgggat ggcgcgcagt tctggcgtca
     4381 ggctcctgaa ccggtgacgg ttatcgacac catgggtgcc ggagattcgt tcattgccgg
     4441 attcctttgc ggctggtctg cggggatgac attaccgcag gcgatagcgc agggaacggc
     4501 gtgcgcggcg aaaaccattc agtaccacgg tgcctggtag gtataacgtt ggcgtgagca
     4561 tcttcacgcc aacgtgctgt tacttgccgg aaaacgaccc tataatccga gtaattcatt
     4621 ctttatttca gggtcgatta tgtcagctac ggaccgctac tctcatcaac tcctctacgc
     4681 taccgtccgc cagcgactgc tggatgatat cgcgcagggg gtttaccagg ccgggcaaca
     4741 gatccctacc gaaaacgagc tttgtacaca atataacgtc agccgcatta ccattcgcaa
     4801 agccatcagc gacttagtgg cagacggcgt actgatccgc tggcagggaa aaggcacctt
     4861 tgtacaaagc cagaaagttg aaaacgccct gcttactgtc agtggtttta ccgattttgg
     4921 cgtctcacaa ggcaaggcga cgaaagagaa agtgatcgaa caggaacggg tcagcgccgc
     4981 gccgttttgc gaaaagctga acatccccgg aaacagcgaa gtgttccatc tctgccgggt
     5041 gatgtatctc gataaagagc cgctgtttat tgatagttca tggatcccgc tgtcgcgtta
     5101 tcctgacttt gatgagattt acgtcgaagg aagctccacc tatcagttat ttcaggagcg
     5161 ttttgacacg cgagtggtca gcgacaaaaa gaccatcgat atctttgccg ccacccgccc
     5221 gcaggcaaaa tggctgaaat gcgaactggg cgaaccgttg tttcgcatca gcaaaatcgc
     5281 ctttgatcag aatgacaaac cggtgcacgt ctccgaactc ttctgccgcg ccaatcgcat
     5341 caccttaact attgataata aaagacatta accgtaggcc ggataagatg cgccagcatc
     5401 gcatccggcg atgctggcgc gttgaatttt acatcccgta cgttcccctc accctaaccc
     5461 tctccccaaa ggggcgaggg gaccgttcag tacggaattt agcttgacgg tgctatccag
     5521 cattaaatac tggcaatact ctcacgcaaa atccgcagca ccgtctcttc accgctgcga
     5581 ttcgggttaa tgcgaatcgc acaatgttct gattgtggat tcgcctggcg aaacgttccg
     5641 gaaaggcgat aaaagagcgg cgggatttca tatttcgact ctgcacccac cgggtaaggc
     5701 aaggcaccgc gcttttgcgc ctcttccagc actctggcgg caatcggctg atgaaactcg
     5761 acaatcaaca ccttcgactg cgcattagca atcaccgcgc ttttcacttc cggcaccgca
     5821 ccaccgttaa gcaaagccag caaccgttca gatacccctg cctgcaccgc gtgcatcact
     5881 ggcgcaaaca ccagaccacg caatacttcc agcgcctgtg cgccctggat ctggctaccg
     5941 ccggagtaaa gcgtggcgcg aatacggttg ataacatcag catcgccgac cactgcacca
     6001 acaccctctg gcccaaatag cttgaagcag gaaaatgtcg agacattcgc gccgcattca
     6061 cagccgattc gagccacctt catcaccgca tagttgtcat cggttaacgc tggaacacct
     6121 gccgcgcgca acgttgccag cacatctgcc agcacgtagc tgtcctgcgg ctgctggcgc
     6181 gtatgctgca ccagcgccgc atccggttgt tgctcgtcga cgacctgctt cagtgccgac
     6241 aggtcattga aatcaacagt aataagcgtc agccccatct gctcaataat aacccgtgtc
     6301 gtcgggtaaa caggcgcgtc atgcaccaga agacgctgcc ccggtttgag caaagccgcc
     6361 agcccggcgc gaatcgcgcc agtccccgcg ccctgcacca gcgccgcagc ctgtgcgtga
     6421 aatgcatcag ccagcacctg ttccacacgc tgggtaacgc gcggttgatt cagtcctggc
     6481 gttaagccta aatcaccgcc agtaagaaac tcgctgccgg ggaaatggcg acaaatgcta
     6541 tccaccagcg caaactgctt ttgctgcgcc tcaataatcg tcaggctttg cagaggaaac
     6601 gtcttcatag gtgccttcct taagccggaa cgaacaggcc aagccagtaa agaacattca
     6661 gcagaatacc ggtgatcatt actgccacga ccggtgccgc cattttctgt accggacggc
     6721 ccagggattc gttgaggaag taaatggcaa ccgcgataga gaatccggta taacccgcca
     6781 tcttaattgc tgcgaaaatc gaaccgacca gcagcgccac ttccatcagc atattcatgg
     6841 cgttacggat gttatccgac gcattacgca ccgacgggta gcgtcccagc catttgccga
     6901 tcgaacgaag cagcaagact tccgccgaaa taaccactgc gcctaatacc gctgcaacca
     6961 tcggattcgg cgagagatag cccaccgcat aaacaaaggt aaagcccgca actgcataca
     7021 cacccgttgc taacgcggtg gtggcaatca acggcacaaa ccccagtccg cgcataaatt
     7081 ctgccagagc cgcctgatta atcagcgttt gcgattgttc cggcgttacg cctgcggaat
     7141 atgctttctc cagtgtgaag atcgacactt cactgccagc aaaaatcttc atgctggcaa
     7201 cggcggcaat caatgctccc acgatggcga tatagggtaa gtttttgata atccgtgacg
     7261 tgcgttcttc aaacaccgaa agcccgctgg catcaatgtc attttcatca cgatgacgca
     7321 ggtcgtgagt tatcgcgatc cccagcagca tcaccatgcc aataaagatt tcgatggatt
     7381 cagggttaag atgtgggaaa tagcgcacga ctaccacacg ggtcatcagt accaccacgg
     7441 cggcgatcag actttgcttc cagccaaact ggtagaaaat cgccaccagc gggaacagtg
     7501 caaaagctga aaccaccggc gagcttaatt cccccaggct acctaataca tccaccggca
     7561 gcgcggtcag cagctggttt actggcaaca ggcaagtaag gatcaacacg ccccagatag
     7621 cgccaagacc aaacgccatc aggctgttta tcgccaggac gccgaggata tcggtaggaa
     7681 gaaagagtaa ccatgcgttg agcagcccgg ttttcagggt aaaagagatc cccaccgagg
     7741 ccacaaaacc aatgctcaga ccaaaagcga tactccccgc ctcgcgacgg ttcatatagc
     7801 cttcaatcag ttgcggcagg atcgggcgga tgccgtcatg aaaaacagcc gccgagcgat
     7861 gcgccagaag cgatgtcata cccgtcaggc acgccaccac gataatctga atatacagat
     7921 ccatagtcgt gccttttatt tcagatggtt aatcagcatc ggaatcgcgt gttcgacgtg
     7981 ctcaacggaa aggccaaacg ccacttttcc ttcagcgatc attttggcga tatgctcgtc
     8041 tttcgcttta atgcctggtt tggcaatggt gcaacttttg ttatagccta tcaccgcgat
     8101 ggcaatcgac aatgccgcgc ccgcaccggt attacacgcg ccgatgtaat aatccagttg
     8161 cccggatttc accttcattg ccgcttccat gtcgttgtga atgaaaactt caaaacagcc
     8221 aggagccgtc gcttcaatag tttttttaat ctgctcacgc tgtaagcctg caacgccaat
     8281 ctttttcatg agtctgtcct tattggaaaa attgagaggg attttcacgt aacatcacat
     8341 ccacatcggc ctgactgaat cctgactggc gcaattgcgg aataaaggtg gttagtaaat
     8401 agtcatagcc ataaccaccg ttggctttta aatgggagcg gcgcgtaata tccatcgaca
     8461 gcatgacgcg gttcagcaac ccacggtcgc gtagcgcatg aagcatcgca atacgctttt
     8521 cgtccgggta gtaactgttc ttgccgatgg tgtcgaactg cacgtacgcg ccgagatcga
     8581 tcatcttcaa aatgttgtcg aggttgtctt tcagatcgca gtgaccaacg gtgacgcgcg
     8641 aaagatcaac cccgtgggct tgtagcaacg ccagttgctc cagccccatc gtgctgaacg
     8701 acgtatgcgt ggagatcggg cgtccggtct ggttatgcgc cagcgcagcg gcaataaata
     8761 ccttctcttc cagcggcgta atctttcctt cgctggtgcc gatctccgcg atgatcccgg
     8821 ctttcagctc cgtgccatcg ataccctgtt caatttcatc gaccatctcc tgcgccagtt
     8881 cctgcacgct gcgggtcgcc acatgttccg ggaaaaacgc gtcctggtaa taaccggtac
     8941 aggccaccac gttgatcccc gtctcgcgca ttacatcaag cataaattgc gcattgcgcc
     9001 ccatgtaacg gttggtcatc tcaatcacat tacgcacgcc ccgggtcatc aggtcgttca
     9061 tctcctggca aatgaacgca tactgatcaa ggcggcagtc cacgttgttt ttaaagccgg
     9121 agagatcaat atgcagatgc tcatgggcga gggtgtaacc cgtcggatca aaactcatag
     9181 ggtgtctcct gcaaaccgga gggaggaaag aaaagagcga ccattttgtg gcggtggcgc
     9241 gcggaaaaat tcacacacgg tagcccccac atcagaaagc gtggtgcgca caccgagctg
     9301 cgtagcgatc atcccttgct gataaaccag cactggcacc acttcgcggg tatggtggct
     9361 gtgaccaatg gtcggatcgt tgccgtgatc cgccatcacg accaggcaat catctggctg
     9421 catcgcctca acaagccggg caaggttacg gtcaacgacc tgcaaacgtt cggcataacg
     9481 tgcgacgtct tctgcatgac cagcgaggtc ggtttcctga atgttggtgc aaataaacgc
     9541 cgtcggatgg gtgttaaatt cgttgagggt gatatccata atccgctggc tatccaccag
     9601 attttgccag ctcacgccat aaggattgtt gacgatatct gccaccttac ccaccagcac
     9661 ggttggcacg cctgcttcat acagtttttg tggcacctgc actttttcat cgacgccata
     9721 gcccatatgc acgacctgga aaccgttgtc ataagcgcca gaacgcggcg cattgatacc
     9781 aataaagcgc ccttctttgc tttctgcggc atcgagaatg cgttgactgt cggttaacag
     9841 gccaccaaat gtaatgaccc gaccgacctg tacctgctca cgcacgatac gaccaatttt
     9901 gattgcgtcg tcaaaagaga tcacagagag attggcggta atgttataga cctggcctaa
     9961 atccgcctcg agattatcgc caatcgcaac cgcctgattg acccacagaa attgcagatc
    10021 atcgccacgg cgctccacct gccagccagc ggaaactaat gcctgctcaa cacggtcaat
    10081 cacatcgcga aaaggcatcc gcagcggcgg taacgggcgc gtgcctaaaa tttcctgatg
    10141 ccccataaag gtatcgccac cttcatgttg cagctctgcc acgccccagg ttgcggaatc
    10201 tgacggctgc atatcgcctg gcgcataacc caatgcgttg attagcccca gcttctccag
    10261 cgttggtagc tgcaaatgcg gcaactggct caggatgtga ccacatgtat tcgctcccgc
    10321 atcttgcgga cgcaccagcg tgacatcttt cattgcccct acgccaaagc tatcaatcac
    10381 taacaccaca aatcgcgcca ttacgccccc agagagtttc caagactgtc ataacgaccg
    10441 acgatttccg gttcgccacg atgaataccg gacaccagca ccacatcgct acgggtgaca
    10501 aaaatctgcg tgcgaaaaca gagcaccact gcactgctta ccggaaactc gcctgccagc
    10561 ggcagggtgt agtcgatact gctgtcatcc acagttttga gattggtttc agtaatcttt
    10621 tgattttctg gcgtaaacac caacgcatgt tgcgcatgac cacgacgata gtaaccgccg
    10681 ccgtagcagt agctgtcgcc acggaaatga tgggagattt cacttaacca gagcatcgcg
    10741 atacgttcag gctgatcgcc ctgctggttt gccggaatag tgcccgtcag cgcatgaccg
    10801 ggttcggcat gagtcacacc gtattgcgcc agtaatggca gcgaagtgca gctggtcgct
    10861 gaaggcgcgt tcagttgctc aagtgcaata ccagatttcg ccagttgatc ccgtgcctgt
    10921 atcagcgtgt gaagattcgg tgtcggcaaa acttttccga cagcctcatc ccaaagcagg
    10981 caagggaaat gggtaagtcc ggctaaatgc agccctggca gattctggat ttcggcgaca
    11041 atctccggca acaccttgag ggcaaaaccg ctctcctggc ccggataaag aaaatcatcg
    11101 tcgctataaa ctttaagcag cacagactgg attcgcccgg ccttcaccgc cgccgcagaa
    11161 acttcccgcg ctttgtcgag agtaaacacg gtgatgacgt cggtgccctg ttcaacggcg
    11221 tcagcaacct gatgacaagg gatttgtacc agatgcccct gatgcgccac aggcaaacca
    11281 gcgcggcgca tgactcgcgc ctctttgtaa tccaccgcca caatgccgct gtagcctaat
    11341 gccagcaatt tttccgccag ccacggatta cgaccaaact gcttggtcat cagatacagt
    11401 tcaatgccgt aaagccgcgc cgtctcaatc agccgcttac cgttttccag tatctgatcc
    11461 acgtcgatca cccagctgtc cggggcgatc ttgccctgct gccacaggct tagtgcggcg
    11521 gaaatcagcg ccgggttctg gcgtttcaat gcttctacaa acatcttgtt gcctcgtttc
    11581 tgttccttga attaaatatt catttttttg aatatttagg ttgtgcgacg tggatttgtc
    11641 gggagcttca aacctcttcg ttagcagcca tccataatcc ataaaggttg gccagcaaat
    11701 agccttcttc acacgggtta acttccagtg cgaattcctt caacaacacc tgatgcaatt
    11761 gcacaacggc ttgccagtgg ctggattgcg ccagttctgc cagcaactcg ttatccagcg
    11821 gctctatttc ttcaccacgg cgactgcgca tcagtgcact cgcatatgtg tcatcgccat
    11881 cgttccttgc tcactgcgca ccggcagatg gcactctgtt tccagtacgt tgacgacctg
    11941 catcatgcct ttgcagatgt ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct
    12001 ggtttccata tcagatgcct ttcgtttaat aactcggttc ctgttcccca ctcactaccc
    12061 gttgttgatg ggcgagcagg gcgtgtttat ctacaacggc acgcagcagt tgttgcattg
    12121 ggtaatcatc gactcgcgtc aggaccacgg cttcggtggc ctgtaaaaat cgcggatcgt
    12181 ctgtgagcgg cgtcgcctct aatcccagca tggtcagttc gttttccgcc accacgttcc
    12241 agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa actctcgtga taagagagat
    12301 cgactcgttc cacatcacta ccgccaaaaa aaacatcggt catgattttc tgatccgccg
    12361 aacggttatc cagccccacg cgcttcacgt ttgcggactc gcctttacgg caaatcaact
    12421 ggtgctcgcc aacgtaggtg tgcggcccca actccagcgc gaggcataag cctttttgcg
    12481 tgagataact ttccgccgct agtcgcgaaa ccaccgccat gtcatacacg ccattaagca
    12541 gacactccac gcgaatatcc gcgccacgca tgtgcgcata gtaaaaagga atgccatcaa
    12601 actgggcttt caatccgctc gccaggcctt cgtacaaacg ggtatagggc aagggcattg
    12661 cacataccac gttgttgata tccacatgag tcagcaatgc tttgttatcc atctcgacca
    12721 gataactgcc attgcgccca cggcgttcaa tccgtatcgc tccgcttgat tccagcgttt
    12781 tcaacgcggc ctgcgtcagg ccaacggatg aacggcattc atttgccagc tcatctatgg
    12841 ttttcaatcg attaccgcac ttttcaccca acaaataacg ggccagcgtc gtgatgacga
    12901 cgccttcttt tttgataaac gttcgacgca taataaattt tcagtaaatt gaatatttat
    12961 atcttcagga atttgaagat aaggcgcaac agcgagatgt ggaaacggcg aggcacttca
    13021 cattttttcg gattattgca aatgaggata aaaaaaccgg gtttccccgg tttcagagtg
    13081 atgataaaag caaaattgcc tgatgcgcta cgcttatcag gcctacattt ccttgcaata
    13141 tgtgcattac tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaagcgc
    13201 aatttgccag caatagtgaa ttacggcttc gccacaaaac caatcgcttc gtacaccgct
    13261 tttagcgtac gggaagcgtg cgcgctggct ttttccgcgc catctttcat cacctgttgc
    13321 aggaaggctt catcgttgcg gaaacggtga tagcgttcct gcaattcagt cagcataccg
    13381 gaaacggcat cagccacttc acctttcaga tgaccataca tcttgccttc gaactgtttt
    13441 tccagttctg ggatgctctg gcccgttacc gctgaaagga tatccaacag gttggaaacg
    13501 cccgctttgt tctgcacatc gtagcgaact accggcggct cgtcggagtc agtgaccgca
    13561 cgtttgattt tcttcactac cgatttcgga tcttccagca ggccgataac gttattgcga
    13621 ttatcgtcag acttggacat cttcttggtc ggctccagca gcgacattac gcgcgcgcca
    13681 gatttcggaa taaacggctc cggcacctta aagatctcgc catacagcgc gttgaaacgc
    13741 tgggcaatat cgcggctcag ttcgaggtgc tgtttctggt cttcacccac cggtaccaga
    13801 ttagtttgat acagcaggat gtccgctgcc atcagcaccg gatagtcaaa cagaccagcg
    13861 ttgatgttct cggcataacg cgcagattta tctttaaact gcgtcatgcg actcagttcg
    13921 ccgaagtagg tatagcagtt cagtgcccag cctaactgtg catgttccgg cacgtgggac
    13981 tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag ccagatacaa ggccagcgta
    14041 tccagcgtcg ctttacgcag cttctgtgca tcctggcgca cggtgatcgc gtgttggtca
    14101 acgatacagt aaatgcaatg gtagtcatcc tgcatgttta cccactgacg cagcgcaccc
    14161 atgtagttac caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac gatgggctta
    14221 gtcatttttc gattcctgat tttcgctatg cggaagccct aatgcgggca gaaggtcatt
    14281 tatagactga taaattacat caggctggct gagatcgata gcctcgccgt agttatatcc
    14341 gtaggttaag ccaactgatg ggcaacctgc cgcttttgcc gcctgaatat cattgcgtga
    14401 gtcgccgaca aacagcatct gttgtggggc aattcccatc cgctcagcca ccagtaacag
    14461 cgggtccgga tgcggttttt tgttttgcac atcatcacca ccaatcacca cgctgaagta
    14521 tttggcgata tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac
    14581 caggcctagc ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcaa cgtgcgggaa
    14641 caaaaacgtc ccctcttcgg caacctcgcc atagtagcga tcgaacagtt tacgcagaat
    14701 acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc ggtttaccca ttgttttacg
    14761 ctgagtcgca cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa catctgcgcc
    14821 gttaccaatc caggtaataa cgcgttcttc acctgcgacg ggcaactcca gcgcatacag
    14881 cgccatatct accgcagcag caagaccagg agcactgtcg accagcgtac catcaagatc
    14941 aaaagcgacg ccgcgaatat cttcaaactt attcatgact tacctttgcc agttcactgc
    15001 gcatttcatc aatgactttt ttgtagtctg gctggtcgaa gattgccgaa ccggcgacga
    15061 acatatccgc gcccgccgca gcgatttcgc caatgttgtt caccttcacg ccaccgtcca
    15121 cttctagtcg aatgtcaaag ccagactcgt cgatacggcg acgtacttcg cgcagtttat
    15181 ccagtgtttg aggaatgaaa gactgaccgc cgaaaccagg gttgacggac atcagcagga
    15241 tcacatccag cttatccatc acgtaatcca gatagctcag aggtgtcgcc gggttaaata
    15301 ccagacccgc tttacagcca ttttctttaa tcagttgcag cgtgcggtca acatgctcgg
    15361 aggcttctgg atgaaaggta atgatgctgg caccagcggc agcgaaatca ggcacaatgc
    15421 gatcgacggg tttcaccatc aggtgtacgt cgataggggc ggtaatgcca tagttacgca
    15481 aggatttcag caccattggc ccaatcgtca gattgggaac atagtggtta tccatgacgt
    15541 caaaatgcac gacatcagcg ccagctgcca gggcttttgc ggtatcttca cccaggcggg
    15601 caaaatcagc cgacagaatt gagggggcaa tcaaatactg tttcatccgc ttctccttga
    15661 gaattatttt ttcgcgggtg aaacgactcc tggtttgtac aaagccagca gttcgtccac
    15721 ctttttacgt gtgccgccgt tgctgcttat actgcgtcga actttgacga catgcaattt
    15781 tgcgcgctga taccactcac gcgttaacat cgtatcgtga ttggagatca gcactggaat
    15841 atggcgctca accagacctt cggcgatctc cgccagatgc gcttgttgtt caagcgtaaa
    15901 actgtttgtg tgatacgccg taaagttggc ggtcgcagac agcggtgcat aaggcggatc
    15961 gcaatagacg acggatgcat catctgcgcg cgccatgcta tcggcgtaag actcacaata
    16021 gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg ggaaataggg
    16081 ttttttgtag cggccgaacg gcacgttaaa ctcaccgcgc agattgtaac gacacaggcc
    16141 gttgtaaccg tagcggttca aatataaaaa cagtaccgcc cgacggaacg gatcctggct
    16201 tttgttgaac tcttcgcgga actgatagta aacctcggcg caatttgttt cgggaacaaa
    16261 cagctcgcgt gcggcctgta cgtactcatc agtacgcatc ttcacaatgt tatagagact
    16321 gatcaggtcg ctattgatat cggcaaggat ataacgagaa aagtcggtgt tgagaaacac
    16381 cgacccggca cctacaaaag gctcaaccag acattcgccc ttgggcaaat gccgtttaat
    16441 atcatcaagc aggggatact tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat
    16501 gctgactaac taattacacc ttctccggct gtggagaaag ctccgacagc atccagcgct
    16561 tcaggcaact cccgcaacga taaaccattg cgggagataa ccttgattac ttcagatcgg
    16621 cctgtacctg acgcagcggt ttcgcccacg ggtttttggc ctggacatct gctggcaatg
    16681 tagataccgc ttttttcgcc tcttctttcg aagcgtacac gccagaaacc aggacatacc
    16741 acggctgacc attacgcgtc gtttcataga caacgtagtt tttcagattc tctttcttcg
    16801 cccaaccgtt caggttgtcg tagttagagg aactgctcag ctgcagagtg taatggctgg
    16861 acggtgccga tttcaacgaa ccaacattac ctgcggtctt cgccccagca gcgggtgttg
    16921 ccgtggtttg cgccggggat gccgtctgta ctggagccgt agtcgccgtc tcttttggtg
    16981 ctggcgtaga agtcgcagcc ggtgccttcg tgctcgccac aggggcagct ggttcagtac
    17041 gcttcggcgt ctgtgctacc ggcttcggct ccgttttcac ggttgcttgc ggttttttcg
    17101 gttcaatcac cgcctgctga cgagctgggc gcgtagtgga cggacgttca gcggtttgcg
    17161 ttttcgccgt gtcacgcgat gcattgccat tgcgaacagg cgcaaccgtt gcgggttcag
    17221 tcggcaacgt ggaattgacc gccacattgt tcagctgttg ctgattttgt ggctgggtca
    17281 gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg accatccgtt gccaccgggg
    17341 tttgcccttg agtcggcgta gaagagatcg gtggcagaga aacatcctgc tgagtatttt
    17401 ccgcagacgt ggttcccggc gctggctgca cgccattcgc ctgatcggtc gcattgccag
    17461 caagatcaat actcttctcg ccagacgcgg tttgatcgct ggaagtggtc gagggggctt
    17521 ttagcgcaga accgataccg atgatcaaca gcagtagaac cagaatgccg acgcccatca
    17581 tcatatactg acgagaagcg ggtttactgg ctgctttttt gcgcttacgc ggacgacgct
    17641 ctacgcgctc ttcatccacg gtttcatctt cggattcgtc aatttcttct tcgatttccg
    17701 gttcctcatt gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt
    17761 caatatcatc aaaattgatc tgcggttcgc cacgttcagt acgctcagaa cgttcagaag
    17821 attgacgaga acgaccagta cgacgatcgc tgggatcggg tttcagctcg tcttctggtt
    17881 tgaattcatc catttaacac cccactaaaa ggttaatgct taccacgttg caattaacct
    17941 gaagctaata gaccgcttga taagcggcct gacctttctt gttgttacgc tgattgacaa
    18001 tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc actcttacca
    18061 attgccaacg gaagaattaa gcgcatctct cccgcaagga ctttcttgtc acgcagcata
    18121 tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa cccagcccgc
    18181 ttgagcaggg ttataatacg ctgcgtttcg gcagaactaa actgcccgag acgttccgac
    18241 gtccgcgccg ccatcaccat acccgcagcg accgcttcac catgtaacca attgccatac
    18301 cccatttcag cttcaatggc atgaccaaag gtgtgtccca gattcagtaa agcacgtaac
    18361 ccggtttcgc gctcgtcggc ggcgacaact tctgccttca gttcacaaca acggcgaata
    18421 cagtacgcca ttgccggacc gtccagacgc aacaacgcat ccagattctc ttccagccag
    18481 ttaaaaaacg caccgtcaag aataatgccg tatttgatga cttctgccag ccccgacgct
    18541 aactcacgcg ggggaagcgt tttcagacag tcgagatcca ccaccactga agcaggttgg
    18601 tagaacgcgc caatcatgtt tttaccgagg ggatggttga ccgcagtttt gccgccaacg
    18661 gaggaatcga cctgcgacag taacgtcgtc gggacttgaa tgaaacggac accgcgctga
    18721 taactcgccg ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag cgccaccagc
    18781 gtagtatcgc gaccatgcgg tttttgtaac aacgccgtaa agacggtatc gagtacagcc
    18841 aggcttttat actgctcgcc gtcagggagg ataacgctat cgacgttaac acccgcctgt
    18901 tcaagtacgc cgcggacctt atcgagatac agaggagcca gggtttcgtt ggtgaccaac
    18961 atgacctgct cgcccgattt cagcggtaag aatgaagctg gttcattaaa caaaccagat
    19021 gcgatggtaa ttgggtaact acgttccccg agagtaacga caatcctctc cataacgcga
    19081 catccacctt aattactgta cccgcagacg agtgtatata aagccagaat tagttgcttt
    19141 ccagcatgtg aataatctgg tttgcaacca ctttagcgct ttgatcatca gtacgaatgg
    19201 tcacgtcggc aatctcttca tacagcggat tgcgttcatt ggccaacgct tccagaactt
    19261 cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa
    19321 gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa agacggttac
    19381 gcgtttcacg ggatttcaca gagccgccgc cagtagccag cacaataccc tgtttctcgg
    19441 tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga
    19501 aaacccagcc cacatcagct ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt
    19561 ccatattgag ttgttgagct aactggcgcc caatagtgct ttttccggca cccataggcc
    19621 caaccagaaa gatattgcgt ttctctgcca ttttttcggt actactaaga ctattcgtta
    19681 atgataaacc cgcttcgctc agagagcgcc gcaggacatg aactgaaacc tcataagata
    19741 ttgcgagagt cagactgaaa attatctcaa tactccagcg ggtttggcaa ctgaataaat
    19801 cacccatccc tctgcatatc tggtcgctgc aagcgcgctg ccttgctacc accgctctgg
    19861 cgataaatca ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa
    19921 atcatcatca tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt actccttgta
    19981 tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc agcgtaaaaa cattaccgct
    20041 tttacggctg tttactcact ggaaaccagt cgtggcgtga taaacaccac taactcgcgt
    20101 cgttcatctt cttttccgtc atgacgaaat aattgcccga accagggaat gtcgccaagc
    20161 aacggtacgc tatcctgacc cgatttattt ttacgggtaa aaatgccgcc cagcgccaac
    20221 gtttctccgc ttttgacctc gacctgcgtt tcgatctcct gcttatcaat cgccagcact
    20281 tcgccatcgg cctgctgtag cacctgcccc ggaacgttct ggctgatgtg taatttcagc
    20341 cggatgcgac ctttttgtaa caccgtgggc gtgacctcca tccccaggac ggcctcttta
    20401 aattccaccg acgtcgcgcc actttccccg ctggaaacct gatatggaat ttcgctcccc
    20461 tgtttaatgc tggcaggctg aagatgtgag gccagcagac gcggactggc gataatatcc
    20521 agctgctgtt tttgttcgag cgcggaaagc tcaagatcca gcaagcgtcc gttgatgcgc
    20581 ccaatgttaa aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct accaagcgtg
    20641 gtgacttgcc caacgccacc agcgtgttgc gcatcggcca gcgtccattt cacgcctaac
    20701 tcacgcaaac ttttttcatt aatggtgaca atatgcgccg acagctcaac ctgcccgacc
    20761 ggcagatcca tttgcgctac ccactgttca agcgcgctta acgccgtttt gttatctcgt
    20821 agcaaaaggc gattggtgcg tttatcgacg gtcatactcc ctttggcact cagtagcttc
    20881 tcccccgctt tcgccagttc tcccgcgtcg gcgtattgca gggttatact gcgattttcc
    20941 agcggcagat ttgcctgcgc ccgcgcctgc tccgcctcct ggcgggcgat attgttattc
    21001 tgccaggcaa tggaatgcac tgagagaatg ttgccttcct gccgcgttat cagtccggcg
    21061 cttttcacta cagtttgtag tgcctgcttc cagggaacat ctgttagatg taacgacacc
    21121 gtaccgctga cgtctggcga cacgaccagg ttcaacttct cctgttcagc cagcgcctgc
    21181 aacacctgag ctaccggaac gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc
    21241 tgtacgccgg gtatcagcat caacagtagt gcggctatcc attgcttcat ttgtatctcc
    21301 ttgccgttgc cacaaccatt gtggcggttc gcagtttgtc ccggtaccca gcgttagtac
    21361 gtctggcgtc agctgtaaaa ttgtccagcc gttttccagc acatcgtttt gctgcacccg
    21421 tcgccatttc ttttgcccgt cttttattac accgatgatg cgctcgcctc gccctaccat
    21481 cccctgatag cgccactggc taagttcgct aatccggcat agatcttccg gcggtttaaa
    21541 agggtcacgc ataccggtta aaaggcacaa tgcaatacct gccaacaacc agcgtttaac
    21601 cctcatgcgg cgtctccagt tgtagcgtga acaaaagatc atcaccttcc acgcttaacg
    21661 aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt
    21721 cccacagcgt tttcaacgcc aactcgcctc cctgcgcgga tggatgccag gaaaccagtt
    21781 gcgcgccgga taactgaaaa tccagtggcg aaaagggcag cgttttttcc tcgctgaagg
    21841 gagtggtgtc taccaggcga tacagtgcgg cccactgctg gtgatgactt gcccgtagtc
    21901 gaattaatgc gtcgcgctct tcatggtgtg tcgatgacag aaaaatgagc gtaactaaca
    21961 tcagcaacca gaatgcccag caaagctggc ggaggcgggg tgatgtggcg aaccaccagt
    22021 caaagaacat gttcatcgct aaccttcctt gttaactgat actcaaattg ccagcgtccc
    22081 tgcgcatcct gctgcgtggc tccccgctga ttgagatgaa aagatgcatc ctggcggagt
    22141 gacgtttcta gtgcgtttaa cgcggtaatg cttgttgtca gccccttgat ctccagcgtt
    22201 ccctgctgcc agcttatcgt tgtcagccag gcgtgctcgg gtaaaagcgc cgccagtgct
    22261 tccagcgcag attgccagtc gcgggtaaat tgtcgctggc gctgccgttg cgaacgctgt
    22321 tcgcgtaact gctgttgctc cagcaaacgt ggcttcgtta tctgtaagct gcgggcgagt
    22381 tgttgttccg cctgaagcaa aactgcgtct atgcgcgctt cggcgctgcc tgtcagacgc
    22441 agtattagcg ttatcccgac ggccagcagc agaggcgcaa cgaacatcag caaccagaaa
    22501 cgcagaaaag cggtccggcg ttgctgtcgc cagggcaaaa aattaattgg cgggttcatc
    22561 agtacgcctt cccaagcgcc agccccagcg cgatggcaaa gtctccacct ggcggcggta
    22621 gcggcggctg acgaacagaa acggcctccc agggatcaaa tccgccttcg ccacatatcg
    22681 cgacgctttc gggatcaacg gataacgctg ccgccagctc cttcgcgcta gtcatcccca
    22741 ccgccagttt gcgcccccag ctatagcgcg tcgcccacag ccactgttcg ttatcacgcc
    22801 aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa tcgctgtaat gcactggcat
    22861 ccggggtgat cgcgctcaca tgaacacgca acctttctgc cagagtaagc agcgttgcca
    22921 gctctttgct ttgcgcggca gtcacgttat aggcgggact gagtgagtct tcgctgtaat
    22981 cgaagcgcag ggagtccgga tccatatcca gctcgcgggc catcgtccct gacagccagg
    23041 ccgtttgctc ccgctcacca agggacatcg acgggcgcgg aaatgaccgc tgtaatgtgc
    23101 gactggcggg aaacgccaac ataatgtgat gacgctgcgg cagttcgcga ctccacggta
    23161 acaacgtttt agccagctgc tgcgcatcaa caatccgccc atctttgata atgtcgttct
    23221 ccagcggcaa ccgccaccag cgttgcaaaa agcattcttt tgcgccccgc acgatcgcaa
    23281 ccgctaccgc ttcttgctgt tgtaaatgca aaccaatttg ccagatctta aatgccattg
    23341 tgatgatctc cttatcaccc gtcactctga cgggtatatc aatgcgtctg gcttgccttt
    23401 atactaccgc gcgtttgttt ataaactgcc caaatgaaac taaatgggaa atttccagtg
    23461 aagttcgtaa agtatttttt gatccttgca gtctgttgca ttctgctggg agcaggctcg
    23521 atttatggcc tataccgcta catcgagcca caactgccgg atgtggcgac attaaaagat
    23581 gttcgcctgc aaattccgat gcagatttac agcgccgatg gcgagctgat tgctcaatac
    23641 ggtgagaaac gtcgtattcc ggttacgttg gatcaaatcc caccggagat ggtgaaagcc
    23701 tttatcgcga cagaagacag ccgcttctac gagcatcacg gcgttgaccc ggtggggatc
    23761 ttccgtgcag caagcgtggc gctgttctcc ggtcacgcgt cacaaggggc aagtaccatt
    23821 acccagcagc tggcgagaaa cttcttcctc agtccagaac gcacgctgat gcgtaagatt
    23881 aaggaagtct tcctcgcgat tcgcattgaa cagctgctga cgaaagacga gatcctcgag
    23941 ctttatctga acaagattta ccttggttac cgcgcctatg gtgtcggtgc tgcggcacaa
    24001 gtctatttcg gaaaaacggt cgaccaactg acgctgaacg aaatggcggt gatagccggg
    24061 ctgccgaaag cgccttccac cttcaacccg ctctactcga tggatcgtgc cgtcgcgcgg
    24121 cgtaacgtcg tgctgtcgcg gatgctggat gaagggtata tcacccaaca acagttcgat
    24181 cagacacgca ctgaggcgat taacgctaac tatcacgcgc cggagattgc tttctctgcg
    24241 ccgtacctga gcgaaatggt gcgccaggag atgtataacc gttatggcga aagtgcctat
    24301 gaagacggtt atcgcattta caccaccatc acccgcaaag tgcagcaggc cgcgcagcag
    24361 gcggtacgta ataacgtgct ggactacgac atgcgccacg gctatcgcgg cccggcaaat
    24421 gtgctgtgga aagtgggcga gtcggcgtgg gataacaaca agattaccga tacgctgaag
    24481 gcgctgccaa cctatggtcc gctgctgcct gccgcagtca ccagcgccaa tcctcagcaa
    24541 gcgacggcga tgctggcgga cgggtcgacc gtcgcattga gtatggaagg cgttcgctgg
    24601 gcgcgtcctt accgttcgga tactcagcaa ggaccgacgc cgcgtaaagt gaccgatgtt
    24661 ctgcaaacgg gtcagcaaat ctgggttcgt caggttggcg atgcatggtg gctggcacaa
    24721 gtgccggaag tgaactcggc gctggtgtcg atcaatccgc aaaacggtgc cgttatggcg
    24781 ctggtcggtg gctttgattt caatcagagc aagtttaacc gcgccaccca ggcactgcgt
    24841 caggtgggtt ccaacatcaa accgttcctc tacaccgcgg cgatggataa aggtctgacg
    24901 ctggcaagta tgttgaacga tgtgccaatt tctcgctggg atgcaagtgc cggttctgac
    24961 tggcagccga agaactcacc accgcagtat gctggtccaa ttcgcttacg tcaggggctg
    25021 ggtcagtcga aaaacgtggt gatggtacgc gcaatgcggg cgatgggcgt cgactacgct
    25081 gcagaatatc tgcaacgctt cggcttcccg gcacaaaaca ttgtccacac cgaatcgctg
    25141 gcgctgggtt cagcgtcctt caccccaatg caggtggcgc gcggctacgc ggtcatggcg
    25201 aacggcggct tcctggtgga cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc
    25261 gtgattttcg aagcgaaacc gaaagtagcc tgcccggaat gcgatattcc ggtgatttac
    25321 ggtgatacgc agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt cgctatctcc
    25381 cgcgagcagc agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta
    25441 gtggcgaaga ctggcgcgca ggagtacgca ccgcacgtca tcaacactcc gctggcattc
    25501 ctgattaaga gtgctttgaa caccaatatc tttggtgagc caggctggca gggtactggc
    25561 tggcgtgcag gtcgtgattt gcagcgtcgc gatatcggcg ggaaaaccgg gaccactaac
    25621 agttcgaaag atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt
    25681 ggctttgatg atcaccgtcg taatctcggt catacaacgg cttccggagc gattaaagat
    25741 cagatctcag gttacgaagg cggtgccaag agtgcccagc ctgcatggga cgcttatatg
    25801 aaagccgttc ttgaaggtgt gccggagcag ccgctgacgc cgccaccggg tattgtgacg
    25861 gtgaatatcg atcgcagcac cgggcagtta gctaatggtg gcaacagccg cgaagagtat
    25921 ttcatcgaag gtacgcagcc gacacaacag gcagtgcacg aggtgggaac gaccattatc
    25981 gataatggcg aggcacagga attgttctga ttaaaaaggc gcttcggcgc cttttcagtt
    26041 tgctgacaaa gtgcacttgt ttatgccgga tacggcgtga acgcgttatc cggctaacaa
    26101 aatcgtgaaa actcaataaa ttgcagaaac cccataagcc tgataaacat tgtgcatcag
    26161 gcaaacttca cgcatttaca ctcgcccctt ccctttcaac cattcgcgca cgaggaacag
    26221 cgcactgaca ttgcgcgctt cattgaagtc agggtcttcc agcaaatcca tcatatgcgc
    26281 cagcggccag cgcacctgtg gtagcggctc tggctcatcg ccttccagtg attccgggta
    26341 gagatcttgc gctaccacga tattcatttt gctggaaaag taagacggtg ccatgctgag
    26401 cttcttcaaa aaagtcagat cgttcgctcc aaatccaacc tcttctttta gctcgcggtt
    26461 agcggcttcg tagacgcttt cacccggatc aattaatcct ttcgaaaaac ctaattcgta
    26521 ggattcagtt cccactgcgt attcgcggat caggatcagg tgatcgtcca caatcggcac
    26581 aatcatcact gcttcccggt tggttggacg cattcgttca taaacacgcc gcacgccatt
    26641 gctgaactcc agatccacgc tctcgacggt aaacagtcgg gaacgggcta cagtttcaac
    26701 attcagaatg gtgggttttt gtaatgattt gctcatcgtg ggatctatgc tgtgaaatca
    26761 gcggttattg tgcgatatcg gacacgcttt cggcaatgtg aattgcatgt tatttacatt
    26821 tatgtaactt aataaataat cgtcctcaaa tcaaattaaa agtcaatagg ttgaaataac
    26881 tccaggaatt tgctgatatt ccgccttcag agggtttgct atgatcagcg gttactgtga
    26941 tgtgcttaat gatgctcaag ttaaactcca cgcttgccga tagccaaccg cagaatcatg
    27001 tattgtgtcc ggtgcgactg accacgcctg acagactaag taagatgggg aaagcatgag
    27061 caccattgtg atttttttag ctgctttgct ggcctgctca ctacttgcgg gatggctgat
    27121 aaaagtgcga tccagacggc gtcagctgcc ctggaccaac gccttcgcgg atgcgcaaac
    27181 gcgtaaactc acacctgaag aacgtagcgc cgttgaaaat tatcttgaga gcctgacgca
    27241 ggtattacag gtgcctggcc caacgggagc cagcgcggca ccgatctctc tggcgctgaa
    27301 tgccgaaagc aacaacgtca tgatgctgac acacgctatc acgcgttacg gcatctctac
    27361 cgacgatccg aataaatggc gttactacct cgattcggta gaagtccacc tgcccccttt
    27421 ctgggaacag tacatcaacg atgagaatac cgttgaactg attcataccg attcgctgcc
    27481 gctggttatt tcactcaacg gtcatacgct gcaggagtac atgcaggaaa ctcgcagcta
    27541 tgccttgcaa cctgttccgt caacgcaggc gtcgattcgc ggggaagaga gtgagcaaat
    27601 cgagctactc aatattcgca aagaaacgca tgaagaatat gcgctgagtc gtccgcgcgg
    27661 gctgcgtgaa gcgttgctga tcgtcgcctc cttcctgatg ttctttttct gcctgattac
    27721 cccggatgta tttgttccgt ggctggcagg cggcgcgtta ctgctgctgg gcgcaggtct
    27781 gtgggggcta ttcgcgcccc cggcaaaatc ctccctgcgg gaaattcatt gtctgcgcgg
    27841 tacaccccgt cgttggggat tgtttggcga aaacgatcag gaacagatca acaatatttc
    27901 actcggtatt atcgacctgg tctatcccgc acactggcag ccatacattg ctcaggatct
    27961 cggtcaacaa accgatatcg atatctatct cgaccgccat gtagtgcgtc agggacgata
    28021 tctttcgctg catgatgaag taaaaaactt tccgttacag cactggctgc gcagtacgat
    28081 tatcgctgcg ggctcgctgc tggtgctgtt tatgctgtta ttctggatcc cgctggatat
    28141 gccgctgaaa ttcactctct catggatgaa aggcgcgcag accattgaag ccaccagcgt
    28201 aaaacaactg gctgacgctg gcgtgcgggt aggcgataca ttgcgtatta gcggtacggg
    28261 aatgtgtaat attcgaactt ccggaacctg gagcgcgaaa accaattcac cttttttacc
    28321 gtttgactgc tcgcagatca tctggaacga cgcccgctca ttgccattac cagaatctga
    28381 actggtcaac aaagcgacgg cattgactga agcagttaat cgccagctgc accctaaacc
    28441 ggaagatgaa tctcgcgtca gtgcctcatt acgttcagca attcaaaaat ccggcatggt
    28501 attgcttgat gattttggcg acattgtact gaagacagcg gatttatgtt ctgccaaaga
    28561 tgactgtgtg cgactgaaaa atgcgctggt caatctcggc aacagtaaag actgggacgc
    28621 gctggtaaaa cgcgccaacg ccgggaagct cgatggcgtg aatgtgttat tacgcccggt
    28681 gagtgcggaa tcgctggata acctggtggc aacctccacc gcgccgttta tcacgcatga
    28741 aacggcgcga gcggcacaat cactaaacag tccggccccc ggcggattcc tgattgtcag
    28801 cgacgaaggc agcgattttg ttgatcagcc ctggccttcg gcatcacttt acgactaccc
    28861 gccgcaagaa cagtggaacg ctttccagaa actggcacaa atgctgatgc atacgccgtt
    28921 taacgccgag ggtatcgtca caaaaatctt cactgacgcc aatggtacgc agcatattgg
    28981 ccttcatccg atcccggatc gttccggcct gtggcgctat ctcagcacca cattgctgct
    29041 actgacgatg ctgggtagcg ccatttacaa tggcgtacag gcctggcgtc gttaccagcg
    29101 tcatcgcact cgcatgatgg agattcaggc ctattatgaa agctgcctga acccgcaact
    29161 gatcacccct tcagaaagcc ttatcgaata acacgtttgc gcggcaggtt atgctaccct
    29221 gtcgcgcaaa ttgcttcact ctggagattt ccctcatgca tatcaacatt gcctggcagg
    29281 acgtagatac cgttctgctg gatatggacg gcacgttgct cgacctcgcc ttcgataact
    29341 atttctggca aaagctggtg cctgaaacat ggggcgcgaa aaacggggtt acgccacagg
    29401 aagcgatgga atatatgcgc cagcaatatc acgacgtaca gcatacgcta aactggtact
    29461 gtcttgatta ctggagtgag caactgggtc tggatatctg tgcgatgacc accgagatgg
    29521 gaccgcgtgc cgtactgcgt gaagatacca ttccgtttct tgaggcactg aaagccagcg
    29581 gtaagcagcg aattttgctc accaatgcgc atccgcacaa cctggcggta aaacttgagc
    29641 ataccggtct ggacgcacac cttgatttat tactttccac ccacacattt ggttatccga
    29701 aagaggatca gcggttatgg catgcggtgg ccgaagctac gggtctgaaa gctgaaagaa
    29761 cgctgtttat tgatgacagc gaagcgattc tcgatgctgc cgcgcaattt ggtattcgtt
    29821 actgcctcgg cgtgactaat cctgattccg ggattgccga gaaacagtat caacgccatc
    29881 cgtcactgaa tgactaccgc cgcctgatcc cctcgctaat gtgaaggaga cgccatgaaa
    29941 gagaaacctg ctgttgaggt tcgactggat aaatggctat gggctgcccg tttttataaa
    30001 acccgcgcgc tggcccgtga aatgattgaa ggcggtaagg tgcattacaa cgggcagcgc
    30061 agcaagccga gcaaaatcgt cgagctgaat gccacgctca ctctgcgcca gggaaatgac
    30121 gaacgcacgg tgattgtaaa ggcgattact gaacagcgtc gccccgccag cgaggcagcc
    30181 ttgctgtatg aagagactgc ggaaagtgta gagaaacgcg aaaaaatggc gctggcacgt
    30241 aaacttaatg ccttaaccat gccgcacccg gaccgacgcc cagacaaaaa agagcgccgc
    30301 gacctgttac gatttaaaca cggcgacagt gaataactgt cacctgcaag agagatgatt
    30361 atgccgcaac atgaccaatt acatcgctat ctgtttgaaa actttgccgt gcgcggcgaa
    30421 ctggtaaccg tttcggaaac cctgcaacag atccttgaga accacgatta tccgcagccc
    30481 gttaaaaacg tgctggcaga actgctggtt gcgaccagcc tgttaaccgc tacgctgaag
    30541 tttgatggtg atatcaccgt acagctgcag ggcgacggtc cgatgaatct ggcggttatt
    30601 aacggtaaca ataaccagca gatgcgcggt gtggcgcgcg tgcagggcga aattccagaa
    30661 aatgccgacc tgaaaacgct ggtcggcaat ggttacgtgg tgatcaccat taccccgagc
    30721 gaaggcgaac gctatcaggg cgtagttggt ctggaaggtg ataccctggc ggcctgcctg
    30781 gaagattact ttatgcgttc tgaacagctg ccgacgcgcc tgtttattcg caccggcgac
    30841 gtagacggca aaccggctgc aggcggtatg ttgttgcagg taatgcctgc gcaaaatgcc
    30901 cagcaggacg actttgacca cctggcgacg ctaaccgaaa ccatcaaaac cgaagaactg
    30961 ctgaccttac cggcaaacga agtgttgtgg cgtttgtatc acgaagaaga ggtgacggtt
    31021 tacgatccgc aggatgtgga gttcaaatgc acctgctcgc gtgaacgttg cgccgatgcg
    31081 ctgaaaacgc tgcctgatga agaagttgat agcatcctgg cggaagatgg cgaaattgac
    31141 atgcattgtg attactgcgg taaccactat ctgttcaatg cgatggatat tgctgaaatc
    31201 cgcaacaacg cgtctccggc agatccgcaa gttcattaat gagtatgtcc ggcagagagg
    31261 gctctgccgg attttacgac taatacacct gatgcgcttt gtcactcatt cagattcaca
    31321 ttcagccttt caccatccac tacaagatcg cgccacgctt taggcttcgt ccccagtttt
    31381 ccatccttcg cagctgtgag taatttttct ttcgttattt cgttcggaag taaactcata
    31441 tcaagcgcgt cccattcttt tctgtctgag tcaaagccat agacaatgac tctgtcatca
    31501 ttaaaagcaa acaggatccg ctccgcctga ccatcactat tcagatcttg ctcgaccagg
    31561 acgcaggcgt ctttttcaat acaggtcatc acgttatagc gatcctggat taaggccgac
    31621 cagaatgtcg catcaggttt aacagaacca ggggcaatta acacattatc ggctaatact
    31681 ttttccgata cctgttgctg aagatgttgc tctccatcta acgccatcag cagatcccgc
    31741 gcgcgtttcg ggtctttcat aaacccggca tcgcttttca gcgactcaag cgcagcacgt
    31801 ccatagcgac cgctctgctc gagcatgtag atagttacct ggtctgacgt gtttttgccg
    31861 ctctgataac gcgccatatg gctgttcacg ctaatgcgca tactgtccag caccggcgaa
    31921 ttaagaagca ccagtatcac caacaccaat aacgaaaccg caaggttcac tttgccctga
    31981 agaacaacgg gattttgccc tttacgccag acgatgctga caaaataccc cagcgaccac
    32041 accagtaaca ccagcaccgc cagcacgccc tgcaagcggt cgacagtcca gccgtactga
    32101 gcgacccgca gccataacgc ccaggcggcg acgaacacat acagcggagc aaccagcaaa
    32161 gcggttttaa tcaggcaacg caacggccct gtccagggaa gtgacgcttt ttgcggatcg
    32221 cggacaatag ccattaagat caattgcaaa aaggccagcg tcaacagcaa cccggcggcg
    32281 gagatgtggc gagaaatcgc gctcaggccc gtaaacggca gggtgatgat aaacatcagg
    32341 gttagcaatg atactaacgg cagcaacccc gtggcgatta acgtgaacaa cttttgaata
    32401 gagtcgatta aacgtgactg tgttcgcgcg aggatcaccg ccagcgcggt aaccagacct
    32461 aacgtgagat aaataaacca gtcggttgca aaaaagagcg tattgaaaaa cgtgatgcca
    32521 acaagtttaa acaactcact ccacagtaaa agcaccagcc acgtcaagcc attggcgaga
    32581 aaaatcacca gtaatataag tacgttatgc catactgact ggtaaaaata gcgatagcgg
    32641 ctactgtcat tgcggatgcg aaggctttgt tgtatccacg gtagcaacaa cattgccatc
    32701 aacagcagat agcaaccaaa atcccaaagc gccttttcag ctctccaggg gttcatgcca
    32761 tcagtttgcc acttcagcca gccgctcatc cccagcgtgg caataaacac tagcgccagc
    32821 catccccaaa ggcgcttctg tttaaaagag atcacggaga agagtaaaac ggatgaaaac
    32881 gcgacggtcg caggcacgcc ataaacaatc caactgtgat tttttccgga aagccaggcg
    32941 atcagcaggt agcaaaccag accctgaagt aatccggtag caatcatccc ccagcgtgtt
    33001 gcgggtgaaa gttcaacgtt atccatgttg gttatccaga atcaaaaggt gggttaatta
    33061 tcgcatccgg gcagtagtat tttgcttttt tcagaaaata atcaaaaaaa gttagcgtgg
    33121 tgaatcgata ctttaccggt tgaatttgca tcaatttcat tcaggaatgc gattccactc
    33181 acaatattcc cgccatataa accaagattt aaccttttga gaacattttc cacacctaaa
    33241 atgctatttc tgcgataata gcaaccgttt cgtgacagga atcacggagt tttttgtcaa
    33301 atatgaattt ctccagatac gtaaatctat gagccttgtc gcggttaaca cccccaaaaa
    33361 gactttacta ttcaggcaat acatattggc taaggagcag tgaaatgcgc gttaacaatg
    33421 gtttgacccc gcaagaactc gaggcttatg gtatcagtga cgtacatgat atcgtttaca
    33481 acccaagcta cgacctgctg tatcaggaag agctcgatcc gagcctgaca ggttatgagc
    33541 gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac cgggatcttc accggtcgtt
    33601 caccaaaaga taagtatatc gtccgtgacg ataccactcg cgatactttc tggtgggcag
    33661 acaaaggcaa aggtaagaac gacaacaaac ctctctctcc ggaaacctgg cagcatctga
    33721 aaggcctggt gaccaggcag ctttccggca aacgtctgtt cgttgtcgac gctttctgtg
    33781 gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac cgaagtggcc tggcaggcgc
    33841 attttgtcaa aaacatgttt attcgcccga gcgatgaaga actggcaggt ttcaaaccag
    33901 actttatcgt tatgaacggc gcgaagtgca ctaacccgca gtggaaagaa cagggtctca
    33961 actccgaaaa cttcgtggcg tttaacctga ccgagcgcat gcagctgatt ggcggcacct
    34021 ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat gaactacctg ctgccgctga
    34081 aaggtatcgc ttctatgcac tgctccgcca acgttggtga gaaaggcgat gttgcggtgt
    34141 tcttcggcct ttccggcacc ggtaaaacca ccctttccac cgacccgaaa cgtcgcctga
    34201 ttggcgatga cgaacacggc tgggacgatg acggcgtgtt taacttcgaa ggcggctgct
    34261 acgcaaaaac tatcaagctg tcgaaagaag cggaacctga aatctacaac gctatccgtc
    34321 gtgatgcgtt gctggaaaac gtcaccgtgc gtgaagatgg cactatcgac tttgatgatg
    34381 gttcaaaaac cgagaacacc cgcgtttctt atccgatcta tcacatcgat aacattgtta
    34441 agccggtttc caaagcgggc cacgcgacta aggttatctt cctgactgct gatgctttcg
    34501 gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac ccagtatcac ttcctctctg
    34561 gcttcaccgc caaactggcc ggtactgagc gtggcatcac cgaaccgacg ccaaccttct
    34621 ccgcttgctt cggcgcggca ttcctgtcgc tgcacccgac tcagtacgca gaagtgctgg
    34681 tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt taacactggc tggaacggca
    34741 ctggcaaacg tatctcgatt aaagataccc gcgccattat cgacgccatc ctcaacggtt
    34801 cgctggataa tgcagaaacc ttcactctgc cgatgtttaa cctggcgatc ccaaccgaac
    34861 tgccgggcgt agacacgaag attctcgatc cgcgtaacac ctacgcttct ccggaacagt
    34921 ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga caacttcgat aaatacaccg
    34981 acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa actgtaatga tttgaagctg
    35041 gagaatatct atccagtatc ttatagaaag caaaacggga ggcaccttcg cctcccgttt
    35101 atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg gcactggcag ccaggcgcga
    35161 atggaaagcc cgccccgctc gctggtgcca agctccagca tcccgttatg gttatccacg
    35221 atacgctgca caattgccag ccctaatccc gtgccgctaa tggtgcgcgc actgtcgccg
    35281 cggacaaacg gctggaacag gtgcttacgt tgttccggcg caattcccgg accgtcatct
    35341 tccacctgga accaggcgcg attcggctcc gttccgctgc tgactttgat ccagccattg
    35401 ccataacggg cggcgttgac caccatattc gccaccgcgc gtttgatcga cagcgggtgc
    35461 attttcactt caatgctgcc ggggtaaagc gcggtttcaa tttcccgctc atagccactt
    35521 tcggcagcaa tcacctcacc gagtactgca ttaagatccg ccatttccat cggcatctcc
    35581 tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg cgttgcactc ttcgatatct
    35641 ttattgatcg attctgccag atagccatcc tgctcgctca tcatctcagt cgccaggcga
    35701 atacgcgtca gcggcgtgcg caagtcgtga cttacccccg ccatcagcag cgtgcggtca
    35761 tccgccagtt gcttaacacc agccgccata tggttaaagg cacgggtaac ggaacgcacc
    35821 tccgaagcgc catactcacg cagcggcggc ggaataatcc ctttaccaac ctgcaaggct
    35881 gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa taaacagcca cgccccgcct
    35941 atcgccaata gcataatcgc cagcgtatag cggaacagcg gagagaaatc gccctgatga
    36001 atttcggtca gcggcacgcg tacccagata ttgggcgaca gccaggtttt cagccagacg
    36061 acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg ggccgcccag ttgctgcgcc
    36121 atctgatggc ttaagaattc atagtgttgc gcccaacgca gacctgcctc ttcggcagcc
    36181 tcgttggagt agagagagat ccccagctca cggtagatct cccgacggaa agcgggaggc
    36241 acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg tcatcaacat acgcacttcg
    36301 tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt cagcaccacc
    36361 agataagtcg tcaccaggct ggcgaacagc aaggtgacga tgagcaataa cgtacgggca
    36421 aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt agagccgtcc ggtacaaaga
    36481 cgtagcccag accccagacg gtctgaatgt aacgcggatg cgctggatct tcttccacca
    36541 tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc cattgcggaa tattcacgac
    36601 cacgggcaag gttcatcagc ttatcgcggg agagcggctc acgcggatgg ctgaccagtg
    36661 ccttcagtac cgcaaactca ccgctggtga gcggcatcgg ctcgtcttcg cggaacattt
    36721 cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat taccgcctct tcctgtgacg
    36781 gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc acggatacgg gccagcagtt
    36841 cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc aatctccagg cctacgatac
    36901 ggtccacttc ttcccctttc gccgtcacca taatgatcgg catcgggttg ctctgactac
    36961 gaagacgtcg gcaaatcgac aagccatctt caccaggtaa cattaaatcc agtaccataa
    37021 gatggaaaga ttcacgagtc agcaggcgat ccatctgttc tgcattagcg acgcttcgaa
    37081 cctggaagcc ttgttcggtg agataacgtt ccagcagcgc acgcaggcgc atgtcgtcat
    37141 cgaccaccag aatcttgtag ttctcttgca ttgtttgtac tcccaaaggt tcgcaacaat
    37201 ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc agctaaatct ggtatgaatt
    37261 tcagcctaaa ttgttacaaa gcatattaaa cagcagctta agtatacaat ttattcggcg
    37321 aaacattatt gattctgttg atatgatcac gttataccca atgtgcgcat tatcaaacag
    37381 acaaagggaa tcaacgagat gaaaacgccc ctggttaccc gggaagggta tgaaaaactc
    37441 aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg aggtcacaaa aaaggtgacc
    37501 tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact atcagtataa taaaaagcgt
    37561 ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat gcctggaaaa tctcaaaatc
    37621 gtcgattact cccctcagca ggaaggcaaa gtcttttttg gcgcgtgggt ggagattgaa
    37681 aacgacgatg gcgtgactca ccgtttccgt attgtcggct acgatgaaat ttttggccgt
    37741 aaagattaca tctctatcga ttccccgatg gcccgcgcat tgctgaaaaa agaagtcggc
    37801 gatctggcgg tggtgaatac ccctgccggg gaagcgagct ggtatgttaa tgctatcgag
    37861 tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat ggctggcatt tttgccagcc
    37921 agtccgtata actatcccct gatttttgat ccgaaaagat gaactcaaac catgatgaat
    37981 gattcgttct gccgcattat tgcgggtgaa attcaggcgc gcccggaaca ggttgacgct
    38041 gccgttcgcc tgcttgacga agggaatacc gtgccgttta tcgcacgtta tcgtaaggaa
    38101 atcaccggcg gtctggatga cacgcagctg cgtaatctgg aaacgcgtct gagctatctg
    38161 cgcgagctgg aagagagacg tcaggcgatc ctcaagtcca tttccgagca aggcaaactc
    38221 accgatgatc tggcgaaggc catcaacgcc accctaagca aaaccgaact cgaagacctc
    38281 tacctgccct acaaacctaa acgccgcacc cgcgggcaaa tcgccattga agcagggctt
    38341 gagccgttgg ctgacctgct gtggagcgat ccgtcacaca cgccagaagt cgccgctgca
    38401 caatatgttg atgccgataa aggcgtggca gataccaaag ccgcgctgga tggcgcgcgc
    38461 tatatcctga tggaacggtt tgccgaagat gccgcgctgc tggcgaaagt gcgtgattat
    38521 ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg gtaaagaaga ggaaggggcg
    38581 aaattccgcg actatttcga tcatcacgaa ccgttgtcca cggtgccttc tcaccgcgcg
    38641 ctggcgatgt tccgtgggcg taacgaaggc gtacttcagc tttcgctgaa tgccgatcca
    38701 cagttcgatg agccgcccaa agagagctat tgcgagcaaa tcatcatgga tcaccttggc
    38761 ctgcgcctga acaatgcccc ggcggatagc tggcgcaaag gcgtggtgag ctggacgtgg
    38821 cgcatcaagg tgctgatgca tctggaaacc gaactgatgg gcaccgtgcg cgaacgtgcg
    38881 gaagatgaag caatcaacgt ctttgcccgt aacctgcacg atctgctgat ggcggcccct
    38941 gccggactgc gtgcaacgat gggcctcgat ccgggtctgc gtactggggt aaaagtggcg
    39001 gtggtcgatg ccactggcaa actggtggcg accgacacca tttacccgca caccggacag
    39061 gccgcaaaag cagcgatgac cgttgctgcg ctgtgtgaaa aacataacgt tgaactggta
    39121 gcgatcggta acggtacagc ttcccgcgaa actgagcgtt tctatctcga tgtgcagaag
    39181 cagttcccga aagtgaccgc gcagaaagta atcgtcagcg aagcaggcgc gtcggtttac
    39241 tcggcttccg agctggctgc acaggagttc ccggatctcg acgtttcgct gcgtggcgcg
    39301 gtgtctatcg cccgccgttt gcaggatccg ctggcggagc tggtgaaaat cgatccgaaa
    39361 tctatcggcg taggtcagta tcagcatgac gtcagccaga cgcaactggc ccgcaaactg
    39421 gacgcagtag tagaagactg cgtaaacgcc gttggcgtcg atctcaacac tgcttctgtt
    39481 ccgctgttaa cccgcgtggc gggcctgacg cgcatgatgg cgcaaaacat cgttgcctgg
    39541 cgcgatgaga acggtcagtt ccagaaccgt cagcaactgt taaaagtgag ccgtctgggg
    39601 ccgaaagcct tcgagcagtg cgcgggcttc ttgcgcatta accacggtga taacccgctg
    39661 gacgcgtcta ccgttcaccc ggaagcctat ccggtggtgg aacgcattct ggcagcaaca
    39721 cagcaggcac tgaaagatct gatgggtaac agcagcgaac tgcgtaacct gaaagcgtct
    39781 gactttactg atgagaaatt cggtgtgccg acggtaaccg acatcatcaa agagctggaa
    39841 aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc agtttgccga tggcgtcgag
    39901 acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg cagtgaccaa cgtcaccaac
    39961 tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc tggttcacat ctcttcattg
    40021 tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag cgggcgacat tgtgaaggtg
    40081 aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc tgactatgcg cctggatgag
    40141 cagcctggcg aaaccaacgc tcgtcgcggc ggcggtaatg aacgcccgca aaacaaccgc
    40201 tcggcagcca aaccacgcgg tcgtgaagcg cagcctgccg gtaatagcgc gatgatggat
    40261 gcgctggcgg cggcaatggg caaaaaacgt taaacgcccg tactggccta cggttcgaat
    40321 ttgcacgaaa tcgtagggca gataaggcgt tcacgccgca tctggcaacg aacgccttgc
    40381 ctgacataaa agtgccggag aatatctccg gcatttttat tccacagcca aactcataat
    40441 atattccggc aatatttatc atttcattaa caactgaaac cttaattaaa cattagccag
    40501 tccgggtaat tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa
    40561 cattgagtca gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga
    40621 aaccattctc attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat
    40681 ctggtctcat gtcgctgtca aacgccccat gaggtagtta tccagttaat gagaaacaag
    40741 taggcaccta tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa
    40801 atcagcccgg catatcgcca aaaactgctt tctcttggca tgttacctgg ctcctctttt
    40861 aatgtggtgc gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc
    40921 ctggtattac gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac
    40981 ggtgataaca acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa
    41041 gacaacgtta tttaaccagc tcactggctc acgtcagcgt gtaggtaact gggctggcgt
    41101 taccgtcgaa cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga
    41161 cctgcccggc acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat
    41221 cgcctgtcac tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc
    41281 taaccttgag cgtaacctgt acctgacgct acaactgctg gaactcggca ttccctgcat
    41341 tgtggcactg aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc
    41401 tctgtcggcg cgtctgggct gtccggtgat cccgctggtt tcaacccgtg gtcgcggtat
    41461 tgaagcgctc aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca
    41521 ttacgcacag ccgctgctca acgaagcaga ttcactggca aaagtgatgc cttccgacat
    41581 cccgctgaaa caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg
    41641 cgcctacgcc ggtgaagcgt cgcagcatct ggatgccgcc ctcgcccgtc tgcgtaatga
    41701 gatggacgat ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg
    41761 tgatgtggta agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa
    41821 aatcgtgctc aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt
    41881 cctgctggct atcaacatcg gcggggcgtt acagccgctg tttgacgtcg gctccgtggc
    41941 gctatttgtg catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac
    42001 tatcttcctc gcccagggcc tgggtggcgg cattaacacc gtgctgccac tggtgccgca
    42061 gattggcatg atgtacctgt tcctctcctt ccttgaggac tccgggtata tggcgcgtgc
    42121 ggcgtttgtg atggaccgtc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc
    42181 gctgatcgtc ggtttcggtt gtaacgtacc gtcggtaatg ggtgcacgta cgcttgatgc
    42241 accgcgtgaa cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct
    42301 ggctatcttc gcagtattcg cggctgcctt cttcgggcag aacggtgcgc tggcggtctt
    42361 ctcgctgtat atgctgggta ttgtgatggc ggtgctgact ggcctgatgc tcaagtacac
    42421 catcatgcgc ggtgaagcga cgccgtttgt catggagttg ccggtctatc atgtaccaca
    42481 cgttaaaagc ctgattatcc agacctggca gcgtctgaaa ggcttcgttc tgcgtgctgg
    42541 taaagtgatc atcatcgtca gcattttcct gagcgctttc aacagcttct cgctgagcgg
    42601 gaaaatcgtc gataacatca acgactcggc gctggcgtcc gtcagccggg tgatcacccc
    42661 ggtcttcaag ccaattggcg tgcatgaaga taactggcag gcaacggttg gcctgtttac
    42721 aggtgccatg gcgaaagaag tggtagtggg tacgctcaac accctctaca ccgcagaaaa
    42781 tattcaggac gaagagttca atccggcaga atttaacctc ggtgaagagc tgttcagtgc
    42841 gatagatgaa acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc
    42901 cattgaagcc agcaaaggcg acggcgaaat gggtaccggg gcgatgggcg tgatggatca
    42961 gaaattcggt agcgcagcag cagcttacag ctacctgatt ttcgtcctgc tgtatgtacc
    43021 atgtatctcg gtgatggggg ctatcgcccg tgaatcaagc cgtggctgga tgggcttctc
    43081 catcctgtgg gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag
    43141 ctacagtcag catccaactt acagcctggt gtgcattctg gcggttatcc tgtttaacat
    43201 cgtggttatc ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac
    43261 ccgcaagtcg gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct
    43321 tcacttattc aggtgcgcga tttgctggcg ttacggggcc gtatggaagc ggcccagata
    43381 agccagacat tgaacactcc acagccaatg attaacgcca tgctgcaaca actggaaagt
    43441 atgggcaaag ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa
    43501 agctgcccgg aaggaaaagc ctgtctgcgc gagtggtggg cgctgcgtta accttactcc
    43561 atcgcctgtt ttggaaagcc cggtatgcgt ctgcatccgg gcttttttgc gtgcggcttt
    43621 ccataaaaat gcaactcttg cagcacggcg taagttcctt tgaaagcatc tcgcagggat
    43681 gaaaactcgc taatacacag gtgtggagtg gcgcgtagag tcgcggcatt caaacaacag
    43741 gtgaaggaac gccatgagca aaaagcagag ttccacccca cacgatgcgc tgttcaaact
    43801 ctttttacgc caaccggaca cggctcgtga ttttcttgcg tttcatttac cggcacccat
    43861 tcacgcgctt tgtgatatga aaaccctcaa gctggagtcg agcagcttta ttgatgacga
    43921 tctgcgtgaa agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata
    43981 catctattgt ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat
    44041 gcgttacgct attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat
    44101 ggtggtgcca ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg
    44161 gctggattgt ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct
    44221 gattgatgtc accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct
    44281 ggagttaatt caaaaacata ttcgtcaacg cgacctgatg gggctggtag agcaaatggc
    44341 ctgcttatta agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat
    44401 actgcaaact ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc
    44461 accgaaacac aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca
    44521 atccaaagcc ctgcatatag ccaaaataat gcttgaatcc ggagttcctc ttgcagacat
    44581 catgcgcttt accgggctgt cagaagaaga gttggctgcg gcgagtcagt aaagttctgt
    44641 ctcgccattt caaaagccac ctacaccctc tgcttcaacg ccaccagcag gtgacaaaac
    44701 tcggccggat gcgaaataaa tggcgcatgg gccgctttgg cgaagatata tgattcgctg
    44761 tgaggccaaa gtttatccag catcggcacc actttgcgcg gcaccagacc gtcgagatag
    44821 ccatacaatc gcaaaaacgg catggacacg ttttgcagcg gctgacggag atcgaccgtt
    44881 ttcaggattt ccagcccgcc attaagcacg tcaacctccg gcatcggtaa cgccagaacg
    44941 gttttcttca acgcccgcgc atcctggcgc gccgtttcag tccccatggt ttgtaacgcc
    45001 aggaaccgct ccactgtacg ctgaaaatca tcactgagtt gctgctgaaa tcccgccagc
    45061 acgtccggtt ttatccccgg ccactcgtca cgagcactaa aacaaggtga cgacgccacg
    45121 gtgaccagcg cctgaacacg ctcgggatgg gttaacgcaa tctggcttgc caccagcccg
    45181 cccagactcc agcctaacca aatggcttta tcaggtgcct gttgcagcac ggcttcggcc
    45241 atatcagcaa gtgacagcgc accaaatccc cggctacgcc cgaagccggg caggtcaaca
    45301 aggtgcagcg taaaatgcga gctaagttcc tcgtcaatgc aacgccacac ttcggcattc
    45361 agtccccatc cgtgcagcag cacaagatga acattcccct gacctttggt ctgccaccag
    45421 atgttattca tccgctattg ttctcttttg acttacaagg atgaacatat gctaacagta
    45481 ccgggattat gctggctatg ccgaatgcca ctggcgttag gtcattgggg gatttgttcg
    45541 gtctgttcac gcgccacccg cacagataaa acgttatgcc cacaatgtgg attacccgcc
    45601 acacactccc atcttccctg cggtcgctgc ctgcaaaaac cgccgccctg gcaaagactg
    45661 gtcacggttg ccgactatgc gccgccgtta agtccgctta tccaccagct taaattttcc
    45721 cggcgcagtg aaatcgccag cgccctgtca cgtctgttac tattggaagt cttacacgct
    45781 cgtcgcacca ccgggttgca attgccggat cgcatcgtca gcgttccgtt atggcagcgg
    45841 cgtcactggc gtcggggatt taatcagagc gatttgctgt gtcagccgtt atcacgctgg
    45901 ttgcactgcc aatgggatag cgaagccgtc acacgtacac gggccactgc gacccagcat
    45961 tttctcagtg cccggctgcg caagcgcaac ctgaaaaatg cctttcgtct tgaattgccc
    46021 gtgcaaggtc gccatatggt gattgtggat gatgtcgtta ccaccggaag taccgtcgca
    46081 gagattgcgc agttgctttt acgcaatggt gcggcggctg tccaggtctg gtgcctttgt
    46141 cgaaccttgt agagcctcga tgatgggcgt attataacca actaaaatag tcaactatta
    46201 ggccattact atgatccgta tttccgatgc tgcacaagcg cactttgcca aactgctggc
    46261 aaatcaggaa gaagggacac aaatccgcgt atttgtgatt aaccctggca cgcctaacgc
    46321 tgaatgtggc gtttcttatt gtccgccgga cgctgtggaa gccaccgaca cagccctgaa
    46381 atttgacctg ctgaccgcgt atgttgatga gttaagcgca ccatacctgg aagatgcaga
    46441 gatcgatttt gttaccgacc agttgggttc ccagttaacg ctgaaagccc cgaacgccaa
    46501 aatgcgtaaa gtggcagacg atgcaccgct gatggagcgc gtggagtata tgctgcagtc
    46561 gcagatcaac ccacagcttg ctggtcacgg tggtcgcgtt tcgctgatgg aaatcaccga
    46621 agacggttac gccattctgc aatttggcgg cggctgtaac ggttgttcca tggtcgatgt
    46681 gacgctgaaa gaagggatcg agaagcagct gctgaacgaa ttcccggagc tgaaaggtgt
    46741 gcgcgatctc accgaacacc agcgcggcga acactcctac tactaagtta tcccctcttt
    46801 tgtagattgc ccgatgcgac gctaaagcgt catttcgggc ctacgttgaa catgtgccag
    46861 acgttggcgc agcattgctt tgtcaaatcc tcggttcgtt tccccgcgat aatatgacca
    46921 acctctcata atttaaattt accccgctct ggtgattctc aaacgccaga tgttacccgt
    46981 atcattcaca tgggtaccaa acatactcct gacatctgac tacaataatt agttttagtg
    47041 ggtatcagtc gtggtgccgc aatatctctg ttcccgattg ggataattag agtttgtcgt
    47101 cagaaaattg acgttaccca taacaaatga aaggccaggt aaatcatgcc attagtcatt
    47161 gttgctatcg gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc
    47221 atcgctctcg tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc gctggataaa
    47281 gttattggct ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc cctgatcatg
    47341 ggttttggcg caatgctggg caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc
    47401 accacgctga ttgccaaatt tggtaaaaaa cacatccagt gggcggtggt actgaccggt
    47461 tttaccgttg gttttgccct gttctatgaa gtgggctttg tgctgatgct gccgctggtg
    47521 tttaccatcg cggcttctgc gaatattcca ctgctgtatg ttggtgtacc aatggcggct
    47581 gcactgtctg tgacccacgg cttcctgcca ccgcatccgg gtccgactgc gattgccacc
    47641 attttcaatg ccgatatggg taaaaccctg ctgtacggta ctattctggc aatcccgacc
    47701 gtgattctcg ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa gccaattccg
    47761 gaaggtctct acagcgcgaa aaccttcagc gaagaagaga tgccgagctt tggcgtcagc
    47821 gtctggacct ctctggtgcc ggtagtactg atggcgatgc gtgcgattgc cgaaatgatc
    47881 ctgccgaaag gtcacgcttt cctgccggta gcggagttcc tcggtgaccc ggtaatggca
    47941 acgctgattg ccgtgctgat tgcgatgttc acctttggtc taaaccgtgg tcgttcaatg
    48001 gatcagatta acgacacgct ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc
    48061 atcggtggtg gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga caaatacatt
    48121 gcttccatga tgcacgaaac caacatttct ccgctgctga tggcctggtc gattgctgcc
    48181 gtactgcgta tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg
    48241 gcaccgctga ttgcaacgac gggtgttagc ccggagctga tggttattgc ggttggttcc
    48301 ggtagtgtga ttttctctca tgtgaacgat ccgggcttct ggctgttcaa agagtacttt
    48361 aacctgacta tcggcgagac catcaaatcc tggtcgatgc tggaaaccat tatctcggtg
    48421 tgcggtctgg taggctgtct gctgctgaat atggtgattt gataacactg actgccggat
    48481 gcggcgtgac cgccttatcc ggcctacgat tcgggatgaa ttagtaggcc ggataagacg
    48541 cgtcaagcat cgcatccggc atcaaccgca ctctacttct tcttcgctgc agctctgcgc
    48601 cgtctgtcca aatccttcag caacttgttc acgccatcat cggcaaacat cgactcaagc
    48661 gttgcggaaa gcttgcgtcg ccagttttta tactggtaac tggtgccagg aatattcacc
    48721 ggttcggcca tatccagcca gtcttccggc tgtagtccta acagagcact gttactgtcg
    48781 gcaatgtagc gctgcaaacc acggttcagc gtcggcgtca tcgacatcaa cgatgcctta
    48841 tgcccggcac gtttcggcag acaaccatat ttatgcagtg catccagcag cccttgcttc
    48901 gccagttcgc gatcctgata cagaccgcgc agtaccactt catccggata cagccccagg
    48961 gttttgccca gcgttagatc cccgctctcc cagtaaccgc gcagcgttgg caggtcatgt
    49021 gtcgccgcaa ccgccatcga ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg
    49081 tggtcgtttt cgaaatagag cactttgtaa gagtacacac cgctgctgcg cagcttaccg
    49141 acaatctcta ccggtacggt accgagatct tcaccaatca ccatacagcg atgacgttta
    49201 ctttcgagtg ccagaatcga gagcagatca tccaccggat agtgaacata cgcgccctga
    49261 tctgccgtct cgccatacgg tatccaccac aaacgcagca tcgacatcac atggtcaatt
    49321 cgtaatgcgc cgcagttttg catattggca cgcaacagct cgataaacgg ttcataggca
    49381 cgcgcggtga tgatatgcgg gtccattggc ggtaatcccc agttctgccc caacgggccg
    49441 aggatatccg gcggcgcgcc aaccgatgct ttcaggcaat atagttcacg gtcacaccag
    49501 gtttccgccc caccttccgc tacgccaacc gccagatcac gatacaagcc aatcggcatt
    49561 tcatagccct ggcttatctc ccagcaggcg gcaaactggc tgtaagccag ccactgcaac
    49621 cagagataaa aatcgacgtc atcacgatgt tcttcgcaga actgacgcac ttctggtgaa
    49681 tccacgttct gatacatctc tggccatgca ggccagcccc agcgcatttc gtcctctttc
    49741 acttgctggg catgtagcgc atcaaaggct gcctgccaga acaggctgtc gccctgctct
    49801 gcaacaaact ggcgaaacgc ggccatctgc tcatcatcac gttgcgcgaa acctttccac
    49861 gccattcgta atgctgtcat ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca
    49921 tcgcgcgcct gttgcagcgt ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct
    49981 tcgctaagat ggaaatcttc aacggcgtta acgtcgatat aaatcacatt cagccaacgg
    50041 cgagaagacg ggctgtatgg gctggcgctc tccggatttg ccggatagag cgcatgaatc
    50101 gggttcaggc caatgaacga cccgccacgt tttgccacat ccaccagcat cgctttgaga
    50161 tcgccaaaat ccccaatacc ccagtttttt tccgatcgca gcgtataaag ctgaacgcag
    50221 gcaccccaca gcttttgttt attcagcaac gcctgcggtt cgtaacagcg tttcggggcg
    50281 acaatcaccc ggcaatgcgc gcgctggtcg tcctgggtga gtgtcagcgt gtgataacct
    50341 tccggcagct tcgtcggtag attgaacgct ttgccccccg ttacatggcc tttgtactgc
    50401 gttccttctt cggtggtcag cagccagcta tattcgccgc tgccctccac caccatcggc
    50461 atttttttgc cgctggtata aaccatgaca ttcgggactg gcgttaccgc cactttcgtg
    50521 gcggtacgtt gatgcatcgc gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc
    50581 ggtttaccgt gggcattgat gtaattgggg ctaatccccg ccgccagcgc ggcattatcc
    50641 agacgtttgc tttccatcga gcttccttag cgttttgcct gccagatacg agcctgataa
    50701 tcgcgaatag agcgatccga gctaaacata ccgcagcggg cggtattgag gatcgccgcg
    50761 cgagtccagg cctcctggtc gcggtacagc acatccacct gcttttgtgc ctctacatag
    50821 gctgcgaaat ccgccatcac cagatacgga tcgccgccct gtttgccgat actgtgcagc
    50881 atctggtcga aggcatgctt atcgccgtcg ctgtatttac cgctttccag ctctttcaat
    50941 actgcgtcca gcaccttatc tttcttccgc catttcaccg ggtcgtagcc tttggccaga
    51001 attgccttca cttgttccac ggtatgacca aaaataaaga tattttcttc accgactttc
    51061 tcggcgattt caacgttcgc cccatccagc gtaccgacag taagcgcacc attgagcgcc
    51121 agtttcatat tgccggtacc ggaagcttct ttacctgcag tcgaaatttg ttcggagata
    51181 tccgccgccg ggatcagttt ttccgccgcc gaaacgcaat aatccggcag gaacaccacc
    51241 ttcaacttat cgccaaccag cggatcgttg ttgatcacgt cagccacttt gttgatcgca
    51301 aagataatat tcttcgccag gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg
    51361 cgcggtacgc gatcagcctg cgggttttca cgaatttctt tgtacaacgc cagaatatgc
    51421 agcagattca ggtgctggcg tttgtactca tgcaaacgtt tgatctgaat atcgaaaatc
    51481 gcctgtggat tgatctcaat accggtacga actttcacaa actccgccag acggacttta
    51541 ttcgcctgct tgatctcgcg atattgctga cggaatttcg catcatcagc gaatttttcc
    51601 agattgatca gctgatcgag atcgttagcc cactcttttt gcagtgattt atccaacaga
    51661 gccgccagtg ccgggttgca ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg
    51721 ttatggaatt tgttcggcca tagctggtga tattccggga acagatcttt caccaccaga
    51781 tccgagtgca gcgccgcaac accgttcacc gcgaaaccgc caaccacaca caggttcgcc
    51841 atatgcactt gtttgtcgtg caccaccgcc agtttggccc acactttttc atcgcccggc
    51901 caggttttct ctaccagcgt tttaaagcga gtattaattt cgttaataat ctgcatgtgg
    51961 cgcggcagta agcctttcac cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg
    52021 gtatggttgg tgtaagcgaa agttttgctg gtaatggccc aggcgtcatc ccagctcatc
    52081 tggtgctcat cgatcagcac gcgcagcagt tctggaatcg cgatagttgg gtgggtatcg
    52141 ttcagctgaa taacttcgta atccgccagt tcgtgcagtt tacgccccgc cagatgatgg
    52201 cgacgcaaaa tatccgctac cgaacaggca cactggaagt attgctgcat caggcgcagc
    52261 tttttaccgg cagtatggtt gtcgtttgga tagagaactt tggtcagttt ttccgcattg
    52321 atgccctgct gttcggcacg caagaaatca ccgtcgttaa atttagtcag atcaaacgga
    52381 tgcgcgtgcg tcgcctgcca cagacgcagc ggctgcgcca cgccattacg atagccgaca
    52441 acggggagat cccacgcttg accggtaatg gtaaactccg gctcccagcg tccgtctttc
    52501 gtcactttac cgccaatccc tacctgcaca tccagtgctt cgttgtggcg gaaccacggg
    52561 tagttactgc gatgccagtc atccggcgct tcaacctgtt tgccatcgac aaaagactgg
    52621 cggaacaaac catattgata gttcagaccg taacccgtcg cagactgacc gacagttgcc
    52681 attgagtcga ggaagcacgc cgccagacgt cccagaccac cgttacccag cgccgggtcg
    52741 atctcttctt ccagcaggtc cgtcagattg atgtcataag ccttcaacga atcctgtaca
    52801 tcctgatacc agccgagatt caacaggttg ttgcccgtca ggcgaccaat caaaaactcc
    52861 attgagatgt agttaacatg tcgctgattc gccaccggct tggcgaatgg ctgagcacgc
    52921 agcatttcgg ccagtgcttc actcactgct agccaccact ggcgaggagt catttcagcc
    52981 acagaattta agccataacg ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg
    53041 ttaaaaatag gttgtgacat aggagttcca cttttcttag attttcaaca caacgttatc
    53101 gctagtttgc caggctcgat gttgaccttc ctcatcctgc gggggattag gcagggagga
    53161 gttgcgggga tgagcaagga aatgtgatct caaccactta aagctagtgc aaaccacagg
    53221 attagcatca aatcaatgca atacagcgca gaaaatctgt atctaagtgc aaaaaatggc
    53281 cgttgcgtat tttcaaaaag cggaaggtaa ctctataaat taagtaaagg agtgaaacag
    53341 tttcataagt aaaatatcca gtgtgctcca tctcattctt aatagattta ttaagatcat
    53401 ctttttagat ggcactttca tcaggaatga agaagaaacc cttgcttaaa tgaatctgat
    53461 gaacataagg gaaaccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata
    53521 aagatttgga attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca
    53581 ttagaaaggt ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat
    53641 ttcctcattt tccacagtga agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc
    53701 ggttcgactc gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa
    53761 caacttccgg ctggcgctga tcacgagtcc tgcgggctac ggaaagacca ccctcatttc
    53821 ccagtgggcg gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag gtgataacca
    53881 gcaagagcgt ttcgccagct atctcattgc cgccgtgcag caggcaacca acggtcactg
    53941 tgcgatatgt gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc
    54001 ccagcttttc attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta
    54061 tcatctgatc actaatccag tgatccacga gtcaatgcgc ttctttattc gccatcaacc
    54121 agaaaatctc accctggtgg tgttgtcacg caaccttccg caactgggca ttgccaatct
    54181 gcgtgttcgt gatcaactgc tggaaattgg cagtcagcaa ctggcattta cccatcagga
    54241 agcgaagcag ttttttgatt gccgtctgtc atcgccgatt gaagccgcag aaagcagtcg
    54301 gatttgcgat gacgtttccg gttgggcgac ggcactacag ctaatcgccc tctccgcccg
    54361 gcagaatacc cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca
    54421 tctttcggat tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt
    54481 tctgttgaaa agcgccattt tgcgctcaat gaacgatgcc ctcatcaccc gtgtgaccgg
    54541 cgaagaaaac gggcaaatgc gcctcgaaga gattgagcgt caggggctgt ttttacagcg
    54601 gatggatgat accggcgagt ggttctgcta tcacccgctg tttggtaact tcctgcgcca
    54661 gcgctgccag tgggaactgg cggcggagct gccggaaatc caccgtgccg ccgcagaaag
    54721 ctggatggcc cagggatttc ccagcgaagc gattcatcat gcgctggcgg caggcgatgc
    54781 gctgatgctg cgcgatattc tgcttaatca cgcctggagt ctgttcaacc atagcgaact
    54841 gtcgctgctg gaagagtcgc ttaaggccct gccgtgggac agcttgctgg aaaatccgca
    54901 gttggtgtta ttgcaggcgt ggctgatgca aagccaacat cgctacggcg aagttaacac
    54961 cctgctagcc cgtgctgaac atgaaatcaa ggacatcaga gaagacacca tgcacgcaga
    55021 atttaacgct ctgcgcgccc aggtggcgat taacgatggt aatccggatg aagcggaacg
    55081 gctggcaaaa ctggcactgg aagagctgcc gccgggctgg ttctatagcc gcattgtggc
    55141 aacctcggtg ctgggtgaag tgctgcactg caaaggcgaa ttgacccgct cactggcgct
    55201 aatgcagcaa accgaacaga tggcacgcca gcacgatgtc tggcactacg ctttgtggag
    55261 tttaatccag caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac
    55321 gcaggaaaaa gcattccagc tgatcaacga gcagcatctg gaacagctgc caatgcatga
    55381 gtttctggtg cgcattcgtg cgcagctgtt atgggcctgg gcgcggctgg atgaagccga
    55441 agcgtcggcg cgtagcggga ttgaagtctt gtcgtcttat cagccacagc aacagcttca
    55501 gtgcctggca atgttgattc aatgctcgct ggcccgtggt gatttagata acgcccgtag
    55561 ccagctgaac cgtctggaaa acctgctggg gaatggcaaa tatcacagcg actggatctc
    55621 taacgccaac aaagtccggg tgatttactg gcaaatgacc ggcgataaag ccgccgctgc
    55681 caactggttg cgtcatacgg ctaaaccaga gtttgcgaac aaccacttcc tgcaaggtca
    55741 atggcgcaac attgcccgtg cacaaatctt gctgggcgag tttgaaccgg cagaaattgt
    55801 tctcgaagaa ctcaatgaaa atgcccggag tctgcggttg atgagcgatc tcaaccgtaa
    55861 cctgttgctg cttaatcaac tgtactggca ggccggacgt aaaagtgacg cccagcgcgt
    55921 gttgctggac gcattaaaac tggcgaatcg caccggattt atcagccatt ttgtcatcga
    55981 aggcgaagcg atggcgcaac aactgcgtca gctgattcag cttaatacgc tgccggaact
    56041 ggaacagcat cgcgcgcagc gtattctgcg agaaatcaat caacatcatc ggcataaatt
    56101 cgcccatttc gatgagaatt tcgttgaacg tctgctaaat catcctgaag tacctgaact
    56161 gatccgcacc agcccgctga cgcaacgtga atggcaggta ctggggctga tctactctgg
    56221 ttacagcaat gagcaaattg ccggagaact ggaagtcgcg gcaaccacca tcaaaacgca
    56281 tatccgcaat ctgtatcaga aactcggcgt ggcccatcgc caggatgcgg tacaacacgc
    56341 ccagcaattg ctgaagatga tggggtacgg cgtgtaagtt tagccggata acgcgccaga
    56401 tccggcttac atctctgcat cattcaatgc tcacccgcgt tacgccatct gtttctatca
    56461 aactaaaccg caccggcaag aaacgctcca ccaccgcgat attggtcagc agatggcatg
    56521 agggatgggc gaccgtaaat tcccccgcgc ccgccagcgc catcggtagc accagttggt
    56581 cagcaagata ttcccccacc gccgccgtgc ttgccaggta gcgtttcacc tctttcacca
    56641 actgtgccgc aaccacctcg gcactgacgc gcttttcacc gacgacaaaa aagcgttcgg
    56701 tgatattttc actttcgact tcaagcgaga cggtattacc cggcccctgg tcgcgcggca
    56761 ggttatgaat attctgttca tgcagggaaa aactccccgc cagtgtagcg atttcacgct
    56821 cagcaacatg gcgcggcaca ccagctaata gaacttctcc acgcatctgc acaatgttcc
    56881 cgcgctcgcc aagttgcaag gtgttaaacg atgccacggg cgagacttcc gttgccacca
    56941 caccgcctcc ggcagggtaa aaaccgtggc gtaacagcgt ggtttgctga tgaattccta
    57001 ttttcgccag cagcggctcc agcacccggc ggataaaatc cgcaggcggc gccgacgggt
    57061 tatcggtgcc gccgctcact tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca
    57121 gcaccgtttg cagcaccagc gtacaacttc cggcgctacc gatagcaaag cggtaatcgc
    57181 cgccgcgcac ggtgccgggc cggaagagca gacgctgcga ccccagctcc gcgccttcca
    57241 cggttgctcc gcaaatttcc gtcgccgcct ttaccgcggt cagatgctgg cgcaacagcc
    57301 ccggtttcgc ccgcccggca cgaatgctgg tgatggtaaa tggctggccg gttatcatcg
    57361 acaggctcag cgccgagcgc aggatctgcc cgccaccttc gccctgtgcg ccatccagcg
    57421 caatcatcct tttcatcatt tatcctttta cgcacaccac ctgacgcagg gtatagataa
    57481 cttccaccag atcgctttgt gccgccatca ccgcatcaat atctttatac gccatcgaga
    57541 tttcgtcgat cacttcggca tctttacggc attccacatg cgcggtggca cgaatttgat
    57601 cttccacgct gaacagtttt ttcgctttag ttcggctcat tacccgcccg gcaccgtggc
    57661 tgcacgaaca gaacgactct tcatttccca gcccacggac gataaagctt tttgctccca
    57721 tcgaaccggg aataattcca tattgaccag cacgcgcaga caccgcgcct ttacgcgtca
    57781 cgtagatctc ttcaccaaag tgctgttctt tttgcacata gttgtggtga cagttgatct
    57841 cttccatcgc cagcgtttgt ggctgtctga ccgttttctg cgtaatgctc tgcaatgccg
    57901 ttaccacgtt ttccatcatc gcatcgcggt taaggctggc aaaaagctgc gcccaggcca
    57961 cggctttcag gtaatcatca aagtattccg taccttccat aaagtacgcc agatcacgcg
    58021 acggcaacgt ctcaagcgtt tcctgcatct ctttttgtgc cagatcgata aagtaagtcc
    58081 cgatggcgtt accaattccg cgtgaaccgg agtgcagcat aatccacacc tggtccgact
    58141 catcaaggca gatttcaata aagtggttac cggttcccag cgttcccagg tgtttatagt
    58201 tattggtatt caggaaacgg ggatattttt gcgttaacca ctgataaccg gcttcaagct
    58261 cagcccattt agcatcgacg ttaacaggtg gattttccca ggcaccttta tcacgtttac
    58321 aacggccagt ggtacgcccg tgcggcacgg ccgtttcaat cgcctgacgc agctctgcca
    58381 ggttttcagg caggtcttcc gccgttaacg cggtacgcag cgcgttcatt ccacagccaa
    58441 tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg ctaccaatgg
    58501 tggaaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa ataaacggca
    58561 tcttcgccgt attaataagt tgctgacgcg catcggcctc taccggcacg cctttggtcc
    58621 acatttttac cggggcattt tcagtggtca gtaattcgta attcattttg tttttctctt
    58681 ttcgttgttt gctgtcctga taattgcaac cgtcgtgcca gaaaattaaa taagcagctt
    58741 aattttttaa ttcattgttt taaaaaagat tatatcttta cgtccgtaac cggagatttc
    58801 ccgcaaagcc aatttaccga taatgaaata tcgtctttta taaggatatc taagatgcgt
    58861 aaaacagtgg cttttggctt tgtcggtacc gtactggatt atgccgggcg cggcagtcag
    58921 cgctggtcaa aatggcgtcc gacactctgt ttatgccagc aagaatcgtt ggtcatcgat
    58981 cgactggaat tgttgcacga cgcccgctcg cgctcgctat ttgaaacgct taaacgcgat
    59041 atcgccagcg tttcgccaga aacagaagtg gtgagcgttg agattgaact gcataacccg
    59101 tgggatttcg aagaggtcta cgcctgcctg catgatttcg cccgtggtta cgagtttcag
    59161 ccagaaaaag aagactattt aattcacatc accaccggta cccacgtcgc gcagatttgc
    59221 tggtttctgc tggcagaagc gcgttacctg cccgcccggc tgatacaatc ttcacctccg
    59281 cgcaaaaaag aacagccgcg cggcccaggt gaagtaacga ttatcgatct cgatttaagc
    59341 cgttataacg ccatcgccag ccgctttgcc gaggaacgcc agcaaacgct tgattttctt
    59401 aagtccggca ttgccacgcg taacccccac ttcaaccgca tgattgagca gatcgaaaaa
    59461 gtggcgatca aatcccgcgc gccgattctg cttaacggtc caaccggcgc gggcaagtca
    59521 tttctggcgc gacgcatctt cgagttaaaa caggcgcggc atcagtttag cggcgcgttt
    59581 gtggaagtga actgcgccac cctgcgcggc gataccgcca tgtcgacgct gtttggtcat
    59641 gtaaaaggcg cgtttaccgg ggcgcgggaa tctcgtgaag gtttattacg cagcgccaac
    59701 ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacgaaca ggcaatgctg
    59761 ctgaaagcca ttgaagagaa aaccttttac ccgtttggca gcgatcgcca ggtgagcagt
    59821 gattttcagc ttatcgccgg aacggtgcgc gatttgcgcc agctggttgc cgaaggcaaa
    59881 tttcgcgaag atctgtacgc gcggatcaat ctctggacct tcaccctgcc gggtctacgc
    59941 cagcgccagg aagatattga accgaacctg gattatgaag tggagcgcca cgcctcactc
    60001 actggcgaca gcgtgcgttt taacaccgaa gcgcggcgcg cctggttggc ttttgcgacc
    60061 tctccccagg caacatggcg cggtaacttt cgcgaacttt ctgccagcgt cacgcggatg
    60121 gccacctttg ccactagcgg acgcatcact ctggacgtgg ttgaagatga gataaaccgt
    60181 ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct gggcgcagag
    60241 gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgttatcgc tatctgccgc
    60301 caggcaaagt cgctttccgc agccggacgc cagctttttg acgtttcgcg ccagggcaaa
    60361 gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg tctgacgtgg
    60421 gaagccgtgc aggatcagca cagctccagt tgaatatggt ggtccgtcag cacctgcatc
    60481 acgcttactg gcggcggggc gtcggtgtag acggcatcta ccatgctgat gctgcccata
    60541 ttgaccatcg cgttacggcc aaatttcgag tgatcgacaa ccagcataac gtggcgcgag
    60601 ttctcaataa tggcgcgttt ggtgcgaact tcgtggtaat cgaactccag cagcgagccg
    60661 tcgctatcga tgccgcttat ccccagaatg ccgaaatcaa ggcggaactg ggagataaaa
    60721 tcgagcgtcg cttcgccaat gatcccgcca tcgcggctgc gtaattcgcc accggcgaga
    60781 atgatgcgaa aatcttcttt taccatcaac gtgttagcaa cgttgagatt gttggtgaca
    60841 atgcgcaaat tgctgtgatt gagcagtgcg tgcgctaccg cttccggcgt ggtgccgata
    60901 tcgataaaca gcgtcgagcc attggggatt tgctccgcca ctttgcgggc gatgcgctct
    60961 ttttcttcgg tctgggtggc cttgcgatcg tgccacggcg tgttaaccga actggaaggc
    61021 agcgccgcac cgccatgatg gcgcaggatc aggttttgct ccgccagctc attgaggtcg
    61081 cggcgaatag tctgcgggct gacggagaaa tgctctacca gctcttcggt actgacataa
    61141 ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt catttataaa
    61201 tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca tcgctaaacc
    61261 cacggctaac ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca aatcaaacca
    61321 tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt aaatgccact
    61381 ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca ccacgccaga
    61441 aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta acagggcgct
    61501 gataagcgta atgacaatta gcttaccgct accgaggcgt ttttccaccg caccgccgag
    61561 ataccaccac cagagcaggt taaagaggat atgcatcagc gagaagtgca ttaacgcgtg
    61621 ggtgaagtaa cgccagaact caaatttcag tgttggatcg aatggccagg ccagccataa
    61681 catcacttcc tgatcgccga gaatttgcat ggcaataaac accaccacgc aggcgatcat
    61741 catcacccag gttaccggac ctgcgcgttc acgcaaggcg gcaaagaaag gataacggcg
    61801 ataatgcagg ccactgccgg tatggcctgc ctgccagctc gccgccagat aacgcggatc
    61861 tgccgggttt tcgagaaaac gcgccagctc cgcccgtacg cgctcggcct gggactcatc
    61921 cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac cctgcgtcgc
    61981 catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa tcatcaacat
    62041 cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc gccgtacgcc
    62101 acctctgcgg gaaactgacg ttgccaggct tcaaagccgc cgtcaatgct atagaccaca
    62161 tcgtagccct gttgcagcag atactgcgcc gcgcctttgc tgctattgcc gtgataacac
    62221 atcaccatca ccggagtgtc aaagtcgtta tcacgcataa aagcgcccag cgtgtcgttg
    62281 gttaaatgga aagcctgcac cgcatgtccc attgcgaaac tctgtggatc gcgaatatcg
    62341 accagcaccg cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt aatacattcg
    62401 aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc gcacagttta
    62461 cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat gttttttcaa
    62521 tgttacctaa agcgcgattc tttgctaata tgttcgataa cgaacattta tgagctttaa
    62581 cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggg ggcggcatca
    62641 atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg atgctggagg
    62701 cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac ggtggcctgc
    62761 gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa cgtgaagtgc
    62821 tgctgaaaat ggccccgcat atcgccttcc cgatgcgttt tcgcctgcca catcgtccgc
    62881 atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat ctgggtaaac
    62941 gcaccagctt gccgggatca actggtttgc gttttggcgc aaattcagtg ttaaaaccgg
    63001 aaattaagcg cggattcgaa tattctgact gttgggtaga cgacgcccgt ctggtactcg
    63061 ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcggact cgcgccacct
    63121 ctgctcgccg cgaaaacggc ctgtggattg tggaagcgga agatatcgat accggcaaaa
    63181 aatatagctg gcaagcgcgc ggcttggtta acgccaccgg cccgtgggtg aaacagttct
    63241 tcgacgacgg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa ggcagccata
    63301 ttgtggtgcc gcgcgtgcat acccagaagc aagcctacat tctgcaaaac gaagataaac
    63361 gtattgtgtt cgtgatcccg tggatggacg agttttccat catcggcact accgatgtcg
    63421 agtacaaagg cgatccgaaa gcggtgaaga ttgaagagag tgaaatcaat tacctgctga
    63481 atgtgtataa cacgcacttt aaaaagcagt taagccgtga cgatatcgtc tggacctact
    63541 ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaggctatt acccgtgatt
    63601 acacccttga tattcatgat gaaaatggca aagcaccgct gctgtcggta ttcggcggta
    63661 agctgaccac ctaccgaaaa ctggcggaac atgcgctgga aaaactaacg ccgtattatc
    63721 agggtattgg cccggcatgg acgaaagaga gtgtgctacc gggtggcgcc attgaaggcg
    63781 accgcgacga ttatgccgct cgcctgcgcc gccgctatcc gttcctgact gaatcgctgg
    63841 cgcgtcatta cgctcgcact tacggcagca acagcgagct gctgctcggc aatgcgggaa
    63901 cggtaagcga tctcggggaa gatttcggtc atgagttcta cgaagcggag ctgaaatacc
    63961 tggtggatca cgaatgggtc cgccgcgccg acgacgccct gtggcgtcgc acaaaacaag
    64021 gcatgtggct aaatgcggat caacaatctc gtgtgagtca gtggctggtg gagtatacgc
    64081 agcagaggtt atcgctggcg tcgtaaatta acgtaaggtg atcaggtcag atttcaatct
    64141 ggcctgagac tgatgacaaa cacaaaactg cctgatgcgc tacgcttatc aggcctacgt
    64201 ggtttatgca atatattgaa tttgcatggt cttgtaggcc agataagacg ttcacgtcgc
    64261 atccggcatg aacaaagcgc actttgtcaa aaatctaacc tactttttaa ttcagggaat
    64321 taccgcaaag cccacgccat catacccaac gtaacaaaac tcaggcacgt tccccactcg
    64381 ccccgagaaa aatagcatta atgcgcccag cgccagcata aaaattttga gcagaaggta
    64441 aattgttggc gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag
    64501 catggcaata accaccgcca aaaattgcgc cagtatgggg atgccgaaaa aagtcattac
    64561 cagcgaggtc aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg
    64621 aaattatact ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa
    64681 aaaaacggga ccctttggcc ccgttctatt tattggtgaa cttacaatct caccggatcg
    64741 atatgccaga tatgatcggc gtactctttg atagtacggt cagaagagaa gtagcccata
    64801 ttggcaatgt tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcatcg
    64861 actttatcct gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg
    64921 aagttgatca gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca
    64981 ccgctgccga tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc
    65041 ggtttgtagc cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag
    65101 atattgtcag caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata
    65161 gtcagcgcac cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc
    65221 agcgaaatct gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg
    65281 tagttcggga tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact
    65341 ttcgctacgt cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc
    65401 ttaccgccaa aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg
    65461 ttatagcggg taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt
    65521 ttgatttgta catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata
    65581 tactctgcca gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt
    65641 gggaaatcac agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg
    65701 cccaggtgtt cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc
    65761 ggcgtcacac cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca
    65821 aacaacgatt gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc
    65881 acaacaaccg ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac
    65941 gcccgtccca gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag
    66001 tcgttgattt caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc
    66061 ggccaggttt ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa
    66121 cacacctcaa acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc
    66181 tcaggaatcg acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg
    66241 ttatcgtagg ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa
    66301 accaggaagt attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga
    66361 tacagtacgc gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca
    66421 ccctggttga atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc
    66481 gtgttggtcg cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc
    66541 tcttcggttt caatccagcg cgttttttta ccttcctgct gaatgcgacc gccaaaacgg
    66601 actttatagc gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag
    66661 tctggcgact ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag
    66721 cggatgccgt aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc
    66781 gccagacgtc ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct
    66841 tcgagattta accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac
    66901 aacatggcgt tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga
    66961 cgagtttctt gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg
    67021 cgcacagcaa ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt
    67081 ccaatcgtaa acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg
    67141 ggcgatgaat atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga
    67201 tagtaaagct cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc
    67261 tgacgttgca caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgtcgaata
    67321 gcccgtaaca gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct
    67381 gcaaggttct caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact
    67441 aacggcagcg taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg
    67501 ctgggcacca gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag
    67561 ccaatctgaa cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc
    67621 agcaccggat cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagacccggt
    67681 aaggcttcca gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa
    67741 agcggcactt tatcgtcaac cttaagcccc attgcgattt gtaactggcg cttattttcc
    67801 gctttatctt ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga
    67861 ctccagattt tctcgtccac gccgttcagt acgccggaaa gacgcccttc acggtgacgc
    67921 tgttgcaaca gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag
    67981 gttggactga ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt
    68041 tgtccgttga attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc
    68101 atgtgatgtg cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc
    68161 gccggacgcc cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca
    68221 tgcaccacat caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac
    68281 cccagcagcg caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac
    68341 gggcttcccg gacgatcata gagatgcggc gcgtcaatca ggtaaatgcc aaccccgttg
    68401 taatgaccga acaacagcgt gatatgtccg gcgaaggtat cacgacggga tactacctgc
    68461 gcatcggtca cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg
    68521 ccgtctgcga tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta
    68581 agcagcggga acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc
    68641 ctaacttccg tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac
    68701 gacgtgcatc ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata acacaagcac
    68761 gatcgatgac gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata
    68821 cggcggaatc aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca
    68881 ccaccaccga accggagatc acacaaccgc cggaaaccag tgagttaagg gtcatcccgt
    68941 ggctaccgga gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa
    69001 ttggccaatt gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgaggttcg
    69061 ctttccagta agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg
    69121 attgtacgca agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg
    69181 gaatcaaatc tttgccaaag tcgtggctgg agttctcatc gcgatcgtct tcttccagca
    69241 gttcatacag atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg
    69301 gatcgttcgg cattgacggc gggttagcag gtttttcaac gaattcgata attttatcgt
    69361 tctcatcaac cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag
    69421 caacggtgca acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct
    69481 tgtagatatg gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt
    69541 cgaggttttg ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct
    69601 gctgtgctgg cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc
    69661 gctgaatgtg ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga
    69721 tcccggagtt gatgcagtta gacagcgcaa agtcgataat gcggaactta ccgccgaagt
    69781 gtacggccgg ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg
    69841 ccagtatcag ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct
    69901 tctctaaact aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg
    69961 tgcaggtccc tgccagacag ccgtaatcac tgggttatcc tctccagcga atgggggaat
    70021 ggcgtgccac tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc
    70081 gatcaaaaag cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc
    70141 cgtgcttaaa ggttgagcat atcgatttag ccaacggaca ttgccgtcgc cttcttccca
    70201 ccagcgattc tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa
    70261 tgcggttaaa ccactgcttg cctgcgacca gtccaaccag gttaattggt tatcctgaca
    70321 gtaggcattg ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg
    70381 cgtaccctgg gagagcaaca acgttgttaa cagggcgtga atgctgtcgc gccgccgttc
    70441 aaccaggtca agagaaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt
    70501 ggtcccgtcg cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca
    70561 gtcgcgaagc gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg
    70621 accattacgt ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg
    70681 caaatcataa tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc
    70741 aaacagcggc gggaaatttc ccacctgata accaccagga gcgatatccc acggttcagc
    70801 aattaacttc acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc
    70861 ctgacggaac tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac
    70921 gtggcaggtt tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg
    70981 actcaaattg agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat
    71041 ccaataatag ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag
    71101 ttccgcacta tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc
    71161 tttgattgca tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg
    71221 cagcgcaaac atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag
    71281 acgtggttca ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg
    71341 tttcaaatag ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac
    71401 cgggatctcc gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct
    71461 gccccacggc gtgcgcggcg gggcatcatc ttcccagtca tagtgatcaa ccaccactac
    71521 gcatttcggc gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag
    71581 cagcgggtta tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt
    71641 cgccgggtta aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata
    71701 acgcaaaccc gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgtgccctgg
    71761 caagtcatag cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc
    71821 atgagcggag aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc
    71881 gggtttgcca atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc
    71941 ggtggtagcg tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt
    72001 accgtgccgc cattgcctgc attactgccg tgatagtgca tggaatcggt attgaggatt
    72061 tcacgccatt tgcccggctg gtttatgccg aagcgataat catgacgcgg taccggcgta
    72121 aagttactgg caacgatgat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc
    72181 gagcgttctt tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca
    72241 tgcattgctt tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg
    72301 tggtgccagt tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac
    72361 tcgcggccct gggcaaattc gttacccatg aacagtagtt tcttgcccgg gaatgcccac
    72421 atccagccat agtaggcgcg caggttcgcg aatttctgcc atgcgtcgcc cggcatgcgg
    72481 tcgagaatcg attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt
    72541 tcagtgtagt tgtagagaat cccgaaggtc agtttatcgt gatgatactg acgataaacc
    72601 gggtcgagct tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag
    72661 aagcccagac cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca
    72721 gccattgtca ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat
    72781 tcaatcgctt caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta
    72841 cggctgtagt cgcgataaat cattgacgcc accgcatcga cgcgcagcgc atcaatacca
    72901 aaacgttcaa tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca
    72961 tagttgtaga tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca
    73021 tacaagttcg tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc
    73081 acccagtcga gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag
    73141 tcgtcgcgag taccaaaacg gcgggttggc gcatacaggc cggttggctg ataaccccaa
    73201 ctgccatcga agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat
    73261 ttagcataag gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg
    73321 gtgtggcgac gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga
    73381 ttcgcttttt tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac
    73441 gcggtttccg ggcgcatttg cgcttcaaag gcataagggt cggacttcag acgcaagttg
    73501 ccattggcat caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac
    73561 agttcccaga tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag
    73621 ttgaattgcc caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta
    73681 cctgtgacgc catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga
    73741 gtaccttcag ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga
    73801 tcatcaatca ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc
    73861 ttacgtcgcg gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg
    73921 agtttgcgcc cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca
    73981 cggacttcca gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa
    74041 tggcctgcaa ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc
    74101 ctgttttatt gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt
    74161 gacctgaacg gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc
    74221 tgtgaatact catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg
    74281 gtaaatcatg aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg
    74341 ccccagattt aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg
    74401 cggcccccca cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat
    74461 tcagcttacg caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta
    74521 gctcacgcat agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag
    74581 ccagcagttc ttccacggtc ggaatagaca catctttttt caatttaata gtgaatgcct
    74641 ggctgtggca gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag
    74701 atgtgttgag gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt
    74761 tatcgagctg tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat
    74821 ccaccggcag ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag
    74881 cagaggacgg ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta
    74941 ataactcacg catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca
    75001 cccaatcaac aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg
    75061 tacagttacc gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct
    75121 gattgacggg gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt
    75181 caatccagta accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat
    75241 cgccgccctg acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat
    75301 cctgaagtgt gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag
    75361 aaaagaagac agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa
    75421 cggagccgac cataccgcgc cagccgataa aaccaacatt tttcataagc gtttttttcc
    75481 tgcaaagatg tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca
    75541 aaaagcagcc aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga
    75601 cttatcctgc tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat
    75661 gaatgaaatc atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct
    75721 acctattttc atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt
    75781 gcgagagttg cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat
    75841 tctggcattt cttagcctac gagcagaaac cgtctccatt tctggcggca tcattctgtt
    75901 tctgatcgcc attaaaatga ttttccccag cgcttcagga aatagcagcg ggcttccggc
    75961 aggtgaagag ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct
    76021 cgccacgctg atgttgttgt ctcatcagta cccgaatcag atggggcatc tggtgattgc
    76081 tctgctgctg gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg
    76141 tctgctgggc gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat
    76201 gatggcaacc cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga
    76261 gtttttcgaa aagaacccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag
    76321 ctggaacgca atcatcccaa cgatggcacc gacagtgccg aggatggttt ccatcatggt
    76381 ccaggttttc agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc
    76441 ggcgtcgtta acgtggctga caacaatcga accaccagcg atacaaatcg acagcgccgc
    76501 catttgcgca ccggagtagt tcagttgttc aataaccggc atcaccagtc ctaccgccgt
    76561 taaacaggct acggtggcag aaccctgaat gatgcgcact gcagctgcca gcacgaagca
    76621 ggtgatagca atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc
    76681 agagtcaacc agcacctgtt tgaacacgcc gcccgcacca atcaccagca gaatgatccc
    76741 cgccggttgc agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg
    76801 cattgccaga ccgtaaatcg ccaccagaca agcaaccaga atcgcggtaa acggatgacc
    76861 gataaactca aaccattcgt aagcagttga tccttccggc acaaaacgcg cggcaatggt
    76921 tttcagccct accagcacca gcggcagcag gatcagcgac aggctgaatc cgaaagatgg
    76981 cattttgcct tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta
    77041 acggctgatg aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca
    77101 caggccaatc aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcatcgg
    77161 cgctggtcca ggcaccagaa atgccgccgc tgccgccacg cctgcaaata atgggattac
    77221 cagcttcacc aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag
    77281 aactatcgcc acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc
    77341 ataatgcgcg cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc
    77401 gccggtttca tgtaagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa
    77461 gccgagggtg cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc
    77521 agaaaaaagg ccagccccca tggacaccac cattaaagcc aggaaagcgt gcatacgcgc
    77581 cttcatgact aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa
    77641 tgtagtcact acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc
    77701 cagcggttga tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag
    77761 cgtttcaaac tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag
    77821 gcggctttca atcacatcaa aatcgccttt caaatagatg aaagagagat tcggattacc
    77881 ttcacgcagc aagtcgcgat agtgtttttt caatgcagaa cagacgatca gcgacacttt
    77941 attagtgcgc tgcatagcaa acgcggcgtc gttcagcgcc tgcaaccacg gtttgcgatc
    78001 gtcgtcattc agtggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa
    78061 atcgccatca agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt
    78121 gccgctgccc gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa
    78181 agtggtgccc ccacaataca agaattaaaa atgttacggg taactgttat cggtaacatt
    78241 gtccagccgg acaatatcag aagcaatatc catccatgcc ttaagtgtat aagtgtgagc
    78301 tacttcaaat ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa
    78361 cattttcggt gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact
    78421 gcccatccgc tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat
    78481 gtcatgaccg tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg
    78541 acattcaaac gccgcgccga ccgccaggtc atcattcgta cagaacacgc catccagctg
    78601 cggatattcc cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg
    78661 ctcaaccatc acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt
    78721 ctgtttgatg atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc
    78781 gcgagcaata atggcagtgg tcatctggcg tgctgcttca aagttatcaa aaccgacggc
    78841 gatatcaagg catggcgact tgctgtccat cagttccacc acgggaatac ccgccacttc
    78901 aatcatcttt aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt
    78961 ccaggagagc atggattcga ggcgttcttg ctccatttcc ggtttataac cgtagtgcgc
    79021 cagcatggtc tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc
    79081 gaaaacctgg ttggtgagag aaggtaacag gacgccaatc gcccggctgg tggcgttaga
    79141 gaggatatcg ggcgcacgat tgggaatata gcccagttca tcaagagccg cggcaatctt
    79201 gccgcgtaga gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt
    79261 ggtcacgcct acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct
    79321 gaaggtacaa aagagataga ttctcttagt ttaacacgga cacatcagaa ccttcccggg
    79381 gaaaacagtg cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc
    79441 taacgacgcg gcaaaacccc ggaagcgtac atcagcacgc gaccggagcg agcgaggaag
    79501 cctgcaaaaa ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca
    79561 gtaacacttc gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca
    79621 gaccatcgct ggtcgaggct ttcacgccgt taatggtgac attgcctttc accacctgga
    79681 tccagacgcg gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc
    79741 agcggtacag ttccatatcc tgatgcactt tcaatgaacc atctcgcgca tccggcgaga
    79801 gcaccagctg tttgccctgt acggcatcga agcgacgctg ttcataacgc ggcgtaatac
    79861 cgttttcttc aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt
    79921 tgtactctga gtgacgaata cccgtaccag cactcataat ctggaactca cccgccggaa
    79981 cctgctcttt attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca
    80041 aaatttccat atctttatgc gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt
    80101 cgttaatcac gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcaaaag
    80161 agaaagtatg ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc
    80221 gtaagtagat catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga
    80281 tctgcctggg atgatagagg gtgaaaattg acccctctgt tcaaaaaaat tgaactattc
    80341 gaggggccat caaacttact tagcgagagt tactgtggag ggagaggctt gctcaaatcc
    80401 gcgttcaagg atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc
    80461 gtgggtgatg gtttgataca acgcatcata aacgcgcccg taatcgccca tctccggctt
    80521 catctcttct ctgaccgtca cgccctcgtc attgacatac tccagcacac cgaccgaatc
    80581 atccgctgcg aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg
    80641 gtcgataccg tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc
    80701 gattttcacc agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc
    80761 ttcaaaggtg tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg
    80821 atccgggcga ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccataaaa
    80881 cgcgccatcc tgcggcagcc caggtttggt ttctgccacc gggcggtaat agtcaaaatg
    80941 gctttccact tcaacaatct ctcccaactt gccactttca atcgcttttt tcgctgtcag
    81001 gaagcaggag tcaaagcgac gattctgata cggcgtgacg gtcagccctt tgcttttcgc
    81061 caacgcaaac agctctttcg cctgcgcaag tgtcggagtg aacggttttt cgaccagcac
    81121 atttttcccg gcttccagcg cgcgtttcgc gtactcgaaa tggctgtccg cgtgggtgca
    81181 gacaacaacc agcttaacat cgggatcgtt tagtacttcg tcgagatcgc tggtgaaatg
    81241 gatatgggaa taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc
    81301 gacatgccag ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt
    81361 gccgaagcca ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt
    81421 tacaccttag cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac
    81481 tcccgtggat aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt
    81541 aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga
    81601 tcgcgagaca ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg
    81661 catttgactc gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca
    81721 aaggaatgag tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc
    81781 agattcacgc ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc
    81841 atatgtggca ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg
    81901 atggagacgt cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg
    81961 ttgccgattt tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc
    82021 tgatgcgaga gatgattgaa atgtgcgaca actggttgcg ggtagatcgc attgaactaa
    82081 ccgtgtttgt cgataacgcc ccggcaatta aggtctataa aaaatacggc tttgaaattg
    82141 aagggactgg taagaagtac gcattgcgta atggtgaata tgtcgatgca tattatatgg
    82201 cgcgggtgaa gtaagatagt gccctttttc tgagatggaa aaagggtgtc attcaaaatc
    82261 gacatacctt cctttaaggt atttatttgc ccaatacata tattgagatt taactcataa
    82321 ttacttccta aagtgtaata ttttattttt taatatatac gcctacaatt tcctggagta
    82381 aataaataac aattaacaag cataatattg ccattgataa aatagcatgc cataaaagga
    82441 cttttcaggg atgagtaata ttgtttacct gacagtaacg ggagaacaac aaggaagcat
    82501 ctccgcaggt tgtgggactt ctgagtctac aggtaatcgt tggcagagcg ggcatgagga
    82561 tgaaatattt acattctcac tcttaaataa tattaataat acggggcttg gttcacagtt
    82621 ccatggtata acattttgta aattaattga taaaagcact ccattattta ttaat
//
LOCUS       DBBGLQ010000011        82635 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.011, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000011 DBBGLQ010000000
VERSION     DBBGLQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.011"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(392..1285)
                     /gene="galF"
                     /locus_tag="ACIYSB_001009"
     CDS             complement(392..1285)
                     /gene="galF"
                     /locus_tag="ACIYSB_001009"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGC5428101.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELGRTQPGAWGRIQLTDAIAELAKKQSVDATLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(1517..2512)
                     /locus_tag="ACIYSB_001010"
     CDS             complement(1517..2512)
                     /locus_tag="ACIYSB_001010"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HGC5428102.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(2670..4064)
                     /gene="wcaM"
                     /locus_tag="ACIYSB_001011"
     CDS             complement(2670..4064)
                     /gene="wcaM"
                     /locus_tag="ACIYSB_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGC5428103.1"
                     /translation="MQFKTLSRRTFLTASSALAFLHTPFARALSARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEDGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKTAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(4075..5295)
                     /gene="wcaL"
                     /locus_tag="ACIYSB_001012"
     CDS             complement(4075..5295)
                     /gene="wcaL"
                     /locus_tag="ACIYSB_001012"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGC5428104.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5292..6572)
                     /gene="wcaK"
                     /locus_tag="ACIYSB_001013"
     CDS             complement(5292..6572)
                     /gene="wcaK"
                     /locus_tag="ACIYSB_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGC5428105.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(6643..8121)
                     /gene="wzxC"
                     /locus_tag="ACIYSB_001014"
     CDS             complement(6643..8121)
                     /gene="wzxC"
                     /locus_tag="ACIYSB_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGC5428106.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVVVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(8123..9517)
                     /gene="wcaJ"
                     /locus_tag="ACIYSB_001015"
     CDS             complement(8123..9517)
                     /gene="wcaJ"
                     /locus_tag="ACIYSB_001015"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGC5428107.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(9653..11023)
                     /gene="cpsG"
                     /locus_tag="ACIYSB_001016"
                     /gene_synonym="manB"
     CDS             complement(9653..11023)
                     /gene="cpsG"
                     /locus_tag="ACIYSB_001016"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGC5428108.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVHDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(11216..12652)
                     /gene="cpsB"
                     /locus_tag="ACIYSB_001017"
     CDS             complement(11216..12652)
                     /gene="cpsB"
                     /locus_tag="ACIYSB_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGC5428109.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(12655..13878)
                     /gene="wcaI"
                     /locus_tag="ACIYSB_001018"
     CDS             complement(12655..13878)
                     /gene="wcaI"
                     /locus_tag="ACIYSB_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGC5428110.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQAFLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(13875..14354)
                     /gene="gmm"
                     /locus_tag="ACIYSB_001019"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(13875..14354)
                     /gene="gmm"
                     /locus_tag="ACIYSB_001019"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGC5428111.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(14357..15322)
                     /gene="fcl"
                     /locus_tag="ACIYSB_001020"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(14357..15322)
                     /gene="fcl"
                     /locus_tag="ACIYSB_001020"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGC5428112.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(15325..16446)
                     /gene="gmd"
                     /locus_tag="ACIYSB_001021"
     CDS             complement(15325..16446)
                     /gene="gmd"
                     /locus_tag="ACIYSB_001021"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGC5428113.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(16473..17021)
                     /gene="wcaF"
                     /locus_tag="ACIYSB_001022"
     CDS             complement(16473..17021)
                     /gene="wcaF"
                     /locus_tag="ACIYSB_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGC5428114.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(17037..17783)
                     /gene="wcaE"
                     /locus_tag="ACIYSB_001023"
     CDS             complement(17037..17783)
                     /gene="wcaE"
                     /locus_tag="ACIYSB_001023"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGC5428115.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(17794..19011)
                     /gene="wcaD"
                     /locus_tag="ACIYSB_001024"
     CDS             complement(17794..19011)
                     /gene="wcaD"
                     /locus_tag="ACIYSB_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGC5428116.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFQVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(18986..20203)
                     /gene="wcaC"
                     /locus_tag="ACIYSB_001025"
     CDS             complement(18986..20203)
                     /gene="wcaC"
                     /locus_tag="ACIYSB_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGC5428117.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(20200..20688)
                     /gene="wcaB"
                     /locus_tag="ACIYSB_001026"
     CDS             complement(20200..20688)
                     /gene="wcaB"
                     /locus_tag="ACIYSB_001026"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGC5428118.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(20691..21530)
                     /gene="wcaA"
                     /locus_tag="ACIYSB_001027"
     CDS             complement(20691..21530)
                     /gene="wcaA"
                     /locus_tag="ACIYSB_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGC5428119.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVSALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKDCLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(21623..23785)
                     /gene="wzc"
                     /locus_tag="ACIYSB_001028"
     CDS             complement(21623..23785)
                     /gene="wzc"
                     /locus_tag="ACIYSB_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGC5428120.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(23788..24231)
                     /gene="wzb"
                     /locus_tag="ACIYSB_001029"
     CDS             complement(23788..24231)
                     /gene="wzb"
                     /locus_tag="ACIYSB_001029"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGC5428121.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKS
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(24237..25376)
                     /gene="wza"
                     /locus_tag="ACIYSB_001030"
     CDS             complement(24237..25376)
                     /gene="wza"
                     /locus_tag="ACIYSB_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGC5428122.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            25685..25834
                     /locus_tag="ACIYSB_001031"
     CDS             25685..25834
                     /locus_tag="ACIYSB_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428123.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            26035..27618
                     /gene="yegH"
                     /locus_tag="ACIYSB_001032"
     CDS             26035..27618
                     /gene="yegH"
                     /locus_tag="ACIYSB_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGC5428124.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRG
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(27892..29745)
                     /gene="asmA"
                     /locus_tag="ACIYSB_001033"
     CDS             complement(27892..29745)
                     /gene="asmA"
                     /locus_tag="ACIYSB_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGC5428125.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLGGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(29767..30348)
                     /gene="dcd"
                     /locus_tag="ACIYSB_001034"
     CDS             complement(29767..30348)
                     /gene="dcd"
                     /locus_tag="ACIYSB_001034"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGC5428126.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(30440..31081)
                     /gene="udk"
                     /locus_tag="ACIYSB_001035"
     CDS             complement(30440..31081)
                     /gene="udk"
                     /locus_tag="ACIYSB_001035"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGC5428127.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            31399..34716
                     /gene="dgcE"
                     /locus_tag="ACIYSB_001036"
     CDS             31399..34716
                     /gene="dgcE"
                     /locus_tag="ACIYSB_001036"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGC5428128.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLAVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGLSIALPLSVAGLSSATLVNELLEQLESSPL
                     PPRLLHLIIPAEAIFDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(34825..35673)
                     /gene="alkA"
                     /locus_tag="ACIYSB_001037"
     CDS             complement(34825..35673)
                     /gene="alkA"
                     /locus_tag="ACIYSB_001037"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGC5428129.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGMVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANVALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            35807..37159
                     /gene="yegD"
                     /locus_tag="ACIYSB_001038"
     CDS             35807..37159
                     /gene="yegD"
                     /locus_tag="ACIYSB_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGC5428130.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLTRILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(37172..39112)
                     /gene="yegI"
                     /locus_tag="ACIYSB_001039"
     CDS             complement(37172..39112)
                     /gene="yegI"
                     /locus_tag="ACIYSB_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGC5428131.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMSKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPAVQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAESRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(39109..39870)
                     /gene="pphC"
                     /locus_tag="ACIYSB_001040"
     CDS             complement(39109..39870)
                     /gene="pphC"
                     /locus_tag="ACIYSB_001040"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGC5428132.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGRGLELPLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(39867..40526)
                     /gene="yegL"
                     /locus_tag="ACIYSB_001041"
     CDS             complement(39867..40526)
                     /gene="yegL"
                     /locus_tag="ACIYSB_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGC5428133.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPQALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(40747..40806)
                     /gene="ibsA"
                     /locus_tag="ACIYSB_001042"
     CDS             complement(40747..40806)
                     /gene="ibsA"
                     /locus_tag="ACIYSB_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGC5428134.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(41078..41134)
                     /locus_tag="ACIYSB_001043"
     CDS             complement(41078..41134)
                     /locus_tag="ACIYSB_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGC5428135.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(41407..41463)
                     /locus_tag="ACIYSB_001044"
     CDS             complement(41407..41463)
                     /locus_tag="ACIYSB_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGC5428136.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            41742..42989
                     /gene="mdtA"
                     /locus_tag="ACIYSB_001045"
     CDS             41742..42989
                     /gene="mdtA"
                     /locus_tag="ACIYSB_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGC5428137.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKSGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQSAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            42989..46111
                     /gene="mdtB"
                     /locus_tag="ACIYSB_001046"
     CDS             42989..46111
                     /gene="mdtB"
                     /locus_tag="ACIYSB_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGC5428138.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYLQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            46112..49189
                     /gene="mdtC"
                     /locus_tag="ACIYSB_001047"
     CDS             46112..49189
                     /gene="mdtC"
                     /locus_tag="ACIYSB_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGC5428139.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            49190..50605
                     /gene="mdtD"
                     /locus_tag="ACIYSB_001048"
     CDS             49190..50605
                     /gene="mdtD"
                     /locus_tag="ACIYSB_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5428140.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            50602..52005
                     /gene="baeS"
                     /locus_tag="ACIYSB_001049"
     CDS             50602..52005
                     /gene="baeS"
                     /locus_tag="ACIYSB_001049"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGC5428141.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPSHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            52002..52724
                     /gene="baeR"
                     /locus_tag="ACIYSB_001050"
     CDS             52002..52724
                     /gene="baeR"
                     /locus_tag="ACIYSB_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGC5428142.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            52915..53247
                     /gene="yegP"
                     /locus_tag="ACIYSB_001051"
     CDS             52915..53247
                     /gene="yegP"
                     /locus_tag="ACIYSB_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGC5428143.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            53394..54755
                     /gene="yegQ"
                     /locus_tag="ACIYSB_001052"
     CDS             53394..54755
                     /gene="yegQ"
                     /locus_tag="ACIYSB_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGC5428144.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQSPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(55085..55402)
                     /gene="yegR"
                     /locus_tag="ACIYSB_001053"
     CDS             complement(55085..55402)
                     /gene="yegR"
                     /locus_tag="ACIYSB_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGC5428145.1"
                     /translation="MNKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            55816..56715
                     /gene="yegS"
                     /locus_tag="ACIYSB_001054"
     CDS             55816..56715
                     /gene="yegS"
                     /locus_tag="ACIYSB_001054"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGC5428146.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     SVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHDITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(56789..57442)
                     /locus_tag="ACIYSB_001055"
     CDS             complement(56789..57442)
                     /locus_tag="ACIYSB_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HGC5428147.1"
                     /translation="MFNCKAVIFDMDGVIIDSEGLWREAQKGALARWGACVSDEECIR
                     LTKGKRLDEIAQTWCEYCHLHIEPALLESEIRRLITTLIAGTGEAMPGVQDVLFFLRR
                     SGYQLALATSSSYQVIDAVLTKLNIQHYFSVICSADDEKHGKPHPAVYLSALRELQLS
                     AADCLVIEDSVSGFQAAQAAGINTIVVSEDAQQTCFNAASARYLSMNELLAALAVAA"
     gene            complement(57487..58764)
                     /locus_tag="ACIYSB_001056"
     CDS             complement(57487..58764)
                     /locus_tag="ACIYSB_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbtT/DalT/CsbX family MFS transporter"
                     /protein_id="HGC5428148.1"
                     /translation="MSLTNKQWLGLPLNLVWGYIAIAIFMTGDGFELAFLSHYIKALG
                     FTPAEASFAFTLYGLAAALSAWVSGVVAEIITPQKTMFIGFVLWCVFHVLFLVFGLGQ
                     ANYGLILLFYGIRGLAYPLFLYAFIVAIVHNVRSDSTSAALGWFWAVYSIGIGVFGSY
                     IPSFTIPLIGEMGTLWLALAFCGVGGAVALISMRKVETPRHMQNLTTKEKFSELGRAV
                     TLLYTNRNILLSSIVRIINTLSLFGFAVIMPMMFVDELGFTTSEWLQVWAVFFFTTIF
                     SNVFWGVLAEKMGWMKVVRWFGCVGMALSSLAFYYMPQHFGHNFAMALIPAVALGIFV
                     AAFVPMAAVFPALEPKHKGAAISVYNLSAGLSNFLAPAIAVVLLPYFSTIGVVIAYTT
                     LYVIAFIICPFIRVEQPGFSNQQVCEGKTATIS"
     gene            complement(58833..60296)
                     /gene="xylB"
                     /locus_tag="ACIYSB_001057"
     CDS             complement(58833..60296)
                     /gene="xylB"
                     /locus_tag="ACIYSB_001057"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGC5428149.1"
                     /translation="MYLGIDLGTSEVKALVIDENNEIVATHSAPLTIQRPHPHWSEQS
                     PQAWWEATEYLMSTLREKCGHHWQAIKAIGLSGQMHGAVLLDEKGEAIRPAILWNDTR
                     SAQECAELEEIAPELHQVAGNLAMPGFTAPKLLWVRRHEPQHFARISTVLLPKDYLRF
                     KMTGQKISDMSDSAGTLWLDVEKRDWSDSLLEKCGLTRANMPELVEGCDVSATLSPEV
                     ASRWGLTSSVVVAGGGGDNAVSAIGVGAVSPGDAFISLGTSGVLFVVTDAYRPAPQSA
                     VHAFCHVLPNLWHQMSVMLSAASCLQWFCRLVGVTETELLEEIAQLSDADKASAPMFL
                     PYLSGERTPHNDPHARGLFWALTHSSQRALMGYAVLEGVSFGIADGLRVLQESGTAIE
                     QCSLVGGGARSPFWAQLLADILDMPVVTHKGGETGGALGAARLACLAAGKPLAAVCEK
                     PEIFKTWRSDPERHNALMQRYQQFTALYHNDLNYRNQ"
     gene            complement(60310..61677)
                     /gene="dalD"
                     /locus_tag="ACIYSB_001058"
     CDS             complement(60310..61677)
                     /gene="dalD"
                     /locus_tag="ACIYSB_001058"
                     /EC_number="1.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047813 - D-arabinitol 4-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0051161 - arabitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinitol 4-dehydrogenase"
                     /protein_id="HGC5428150.1"
                     /translation="MNEQFTWLHIGLGSFHRAHQAWYLHRLQVMGDKRWSIAAGNIRN
                     DAEHVVQALSAQKGRYVLETVSPEGVSEYEEITSIQKLIPWQADLQPLIAEGADPKTK
                     VIAFTVTEGGYYLNTSHKLEVNNPDLAADLKGGCKTIYGVITRILEARMANNAGPLTL
                     LNCDNVRHNGERFHDGLVEFLQLTGKQDVIDWLSTNTTCPNTMVDRITPRPAAELPAR
                     IKAQTGIADKAPVMGETFIQWVVEDNFRDVRPALEKVGVELVASVIPYEEAKIRILNS
                     SHSCIAWAGTLIGQKYIHESTMTDFIYQIADRYVTEDVIPCLGDNGIDLPTYRDVVLK
                     RFTNPHIQDTNQRVAADGFSKIPAMIAPTLRECYQRGVRPNATAMLPALFYVFMEQWH
                     HGKLPYEYQDGILDAPAVHAMFQSADPVAVYASDKALFGDLTEREDFAALLREKIADV
                     YALIN"
     gene            61890..62831
                     /locus_tag="ACIYSB_001059"
     CDS             61890..62831
                     /locus_tag="ACIYSB_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGC5428151.1"
                     /translation="MNKEDDIRLDQKVRAAWMYYIAGLNQSEIASQLGTSRPVVQRLI
                     AAAKDEGIVSIGLHHPVANCLDYAQLLKEKYQLTDCNIMPVYSIESTLDSVTFGCYQL
                     MARYLQGDKPTIVGIGSGLTLKKTIKRIDFDSQNTRCVALISAMNEHGQCNYYDDVPL
                     LLARKIQGKYYQWPAPRYAQSREEYQMWSTNRLFRNVSTVARDSDVIFVGIGALGRQS
                     PIFKDGFIDEQQLEELTARGGVGEILGRFIDAEGNVVESQINQMITSHDIRESNCPRI
                     AAACGEDKRPAILAALKGGWINGLVTDEHTARWLLTR"
     gene            complement(62834..63865)
                     /locus_tag="ACIYSB_001060"
     CDS             complement(62834..63865)
                     /locus_tag="ACIYSB_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGC5428152.1"
                     /translation="MTRVEKTKKTTIYDLAELTGVSASAVSAILNGNWKKRRISAKLA
                     EKVTRIAEEQGYAINRQASLLRSKKSNVIGMIVPKYDNRYFGSIAEKFEEMARARGLL
                     PIITCTRRQPELEIEAARAMLSWQVDWVIATGATQPDKIAQMCQQAGVPTLNLDLPGT
                     LAPSIISDNYMGAKLLTERILANSQKRYGKLAPLTFVGGRISDNNTAERLCGFVDAHQ
                     ALGITVPDEHKLVTGYSSARVEGLLKEVFQSSAVSLHGLFVNSTISLEGVVRWLAQTG
                     QTGANQPPMGCFDWDPFVSLLGHDIDMVRQDVPAMLEKVFEIVDSGDTTLRRIEIPPQ
                     LIPSLNVVS"
     gene            64027..64776
                     /locus_tag="ACIYSB_001061"
     CDS             64027..64776
                     /locus_tag="ACIYSB_001061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGC5428153.1"
                     /translation="MNHSVPSMNTPLNGKVAAITGAASGIGLQCAKTLLDAGAKVVLI
                     DREGDKLHKIVAELGENAYALQLDLFNNQQVDNMLADIIELAGGLDIFHANAGAYIGG
                     PVAEGDPDVWDRVLNLNINAAFRCVRAVLPHMIAQRSGDIIFTSSIAGVVPVIWEPIY
                     TASKFAVQAFVHTTRRQVSQYGVRVGAVLPGPVVTALLDDWPKAKMEEALANGSLMQP
                     IEVAESVLFMVTRSKNVTVRDLVILPGSVDL"
     gene            64787..66391
                     /locus_tag="ACIYSB_001062"
     CDS             64787..66391
                     /locus_tag="ACIYSB_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family pentulose kinase"
                     /protein_id="HGC5428154.1"
                     /translation="MTITKTVIGVDVGSGSVRAGIFDLNGSLLSHATEKITTTRRSGS
                     RVEQSSQEIWQAVCSCIRNALTLADVCAQSVAGIGFDATCSLVVLDKNGDPLPVSPEG
                     DAKQNIIVWMDHRATEQAERINATHHPVLNYVGGKISPEMETPKILWLKENMPEIYER
                     AGQFFDLADFLTWRATGDLARSVCTVTCKWTWLAHENRWDPDYFRTIGLAELADEDFI
                     RIGHHIVSPGTPCGNGLTAQAAAEMGLLPGTPVAVGLIDAHAGGIGTVGVEGGALNNL
                     AYVFGTSSCTMASTTSPSFVPGVWGPYYSAMVPGLWLVEGGQSAAGAAIDQLLDFHPA
                     VEEAREMAQRVNQPLPVWLADRILEKTAQPSDAVALAKGLHVVPEFLGNRAPFADPHA
                     RAVICGLGMERDLDNLLALYIAGLCGIGYGLRQILDAQTAQGVVSKNIVISGGAGQHP
                     LVRQILADTCGIPVITTQCCEPVLLGSAILGAVAGNIAPSVGEAMQQFTHVDKYYYPQ
                     ERYQSLHHRRYEAYKQLQHTAKLLRD"
     gene            66493..67767
                     /locus_tag="ACIYSB_001063"
     CDS             66493..67767
                     /locus_tag="ACIYSB_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbtT/DalT/CsbX family MFS transporter"
                     /protein_id="HGC5428155.1"
                     /translation="MSRNNKQWLGLPLHLIWGYIAIAVFMTGDGFELAFLSHYIKSLG
                     FTPAEASFAFTLYGLAAALSAWVSGVVAEIITPQKTMLIGFVLWCVFHVLFLVFGLGQ
                     ANYGLILLFYGIRGLAYPLFLYSFIVVIIHNVRSENSSSALGWYWAVYSVGIGVAGSY
                     IPSFTIPIMGEMGTLWLALAFCFAGGVIAMISLRHVKTPGHMHNLTPREKFAELSRAV
                     TLLYTNRNIFLSSIVRIINTLSLFGFAVIMPMMFVDELGFTTSEWLQVWAAFFFTTIF
                     SNIFWGIVAEKMGWMRVIRWFGCLGMAASSLAFYYMPQYFGHNYWMAMIPAIALGTFV
                     AAFVPMAAVFPALEPKHKGAAISVYNLSAGMSNFLAPAIAVVLLPWFSTIGVVIAYTA
                     LYLLAFVLCAFIRVEQPGFSSAPVTEKALNIS"
     gene            complement(67824..68876)
                     /gene="fbaB"
                     /locus_tag="ACIYSB_001064"
     CDS             complement(67824..68876)
                     /gene="fbaB"
                     /locus_tag="ACIYSB_001064"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGC5428156.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDNKVTIA"
     gene            69133..70410
                     /gene="yegT"
                     /locus_tag="ACIYSB_001065"
     CDS             69133..70410
                     /gene="yegT"
                     /locus_tag="ACIYSB_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGC5428157.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            70407..71411
                     /gene="yegU"
                     /locus_tag="ACIYSB_001066"
     CDS             70407..71411
                     /gene="yegU"
                     /locus_tag="ACIYSB_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGC5428158.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPIAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAIAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            71408..72373
                     /gene="yegV"
                     /locus_tag="ACIYSB_001067"
     CDS             71408..72373
                     /gene="yegV"
                     /locus_tag="ACIYSB_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGC5428159.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKSAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(72347..73093)
                     /gene="yegW"
                     /locus_tag="ACIYSB_001068"
     CDS             complement(72347..73093)
                     /gene="yegW"
                     /locus_tag="ACIYSB_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGC5428160.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(73145..73963)
                     /gene="yegX"
                     /locus_tag="ACIYSB_001069"
     CDS             complement(73145..73963)
                     /gene="yegX"
                     /locus_tag="ACIYSB_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGC5428161.1"
                     /translation="MQLRITSRKKFTALLCVLGLISIIAIYPRQTVNFFYSTAVQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGMLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(74028..74828)
                     /gene="thiD"
                     /locus_tag="ACIYSB_001070"
     CDS             complement(74028..74828)
                     /gene="thiD"
                     /locus_tag="ACIYSB_001070"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGC5428162.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARNEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(74825..75613)
                     /gene="thiM"
                     /locus_tag="ACIYSB_001071"
     CDS             complement(74825..75613)
                     /gene="thiM"
                     /locus_tag="ACIYSB_001071"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGC5428163.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(75648..75744)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(75836..76108)
                     /gene="rcnR"
                     /locus_tag="ACIYSB_001072"
     CDS             complement(75836..76108)
                     /gene="rcnR"
                     /locus_tag="ACIYSB_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGC5428164.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            76229..77053
                     /gene="rcnA"
                     /locus_tag="ACIYSB_001073"
     CDS             76229..77053
                     /gene="rcnA"
                     /locus_tag="ACIYSB_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGC5428165.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            77272..77610
                     /gene="rcnB"
                     /locus_tag="ACIYSB_001074"
     CDS             77272..77610
                     /gene="rcnB"
                     /locus_tag="ACIYSB_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGC5428166.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(77692..78726)
                     /gene="yehA"
                     /locus_tag="ACIYSB_001075"
     CDS             complement(77692..78726)
                     /gene="yehA"
                     /locus_tag="ACIYSB_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HGC5428167.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLEGIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(78742..81222)
                     /gene="yehB"
                     /locus_tag="ACIYSB_001076"
     CDS             complement(78742..81222)
                     /gene="yehB"
                     /locus_tag="ACIYSB_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGC5428168.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSEFVQVGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(81238..81912)
                     /gene="yehC"
                     /locus_tag="ACIYSB_001077"
     CDS             complement(81238..81912)
                     /gene="yehC"
                     /locus_tag="ACIYSB_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGC5428169.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLDSQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(81992..82534)
                     /gene="yehD"
                     /locus_tag="ACIYSB_001078"
     CDS             complement(81992..82534)
                     /gene="yehD"
                     /locus_tag="ACIYSB_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5428170.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTSVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT        19637 a        21729 c        20654 g        20615 t
ORIGIN      
        1 agtaagaatt catttttctg tgttcaatat gaacgactct aactttttta acattcatta
       61 aacgtaaacg aacgactcat tacacacagt tgcgattaat tattcgaaga atacaacgct
      121 accgcccctg gctttaaagc taccagcacg ctacgcactt ccaacgaatt gtatatactc
      181 ctattcctct aaaattatcc ctagattctc ttactgatta gctattgtta acttttagaa
      241 caatcatttc cgcgaaacct tgtgatcaga atccttttat taatcgttgt tatactgcat
      301 atccatataa aaatacggca cgaatattta cactaaatga gctcctccgc cgccataatt
      361 gccttatcag ccactacact aggtcatatc tttattcgct taacagcttc tcaatacctt
      421 tgcggaactt cgcaccttct ttcaggttgc gtaatccata cttcacaaat gcctgcatat
      481 aacccatttt tttaccgcag tcgtagctgt ccccagtcat cagcgttgca tcaacggact
      541 gttttttcgc cagttcagca atggcatcag tcagctgaat acgcccccat gcaccaggct
      601 gagtgcgtcc aagttccggc caaatatcgg cagaaagcac atagcgacca acggccatga
      661 tgtctgagtc cagcgtctgc ggctgatcag gtttttcgat aaattcaaca atgcggctga
      721 ctttgccttc acgatccagc ggctctttgg tctggatgac ggagtattca gagaggtcac
      781 ccggcatacg ttttgccagc acctggctgc gtcccgtttc gttgaagcgc gcaatcatgg
      841 ctgcaaggtt gtagcgcagc gggtcggcgc tggcgtcgtc gatcacaacg tctggcagca
      901 ccacgacaaa tgggttgtca ccaatggcgg gtcgtgcaca taaaatggag tggcctaaac
      961 ctaaaggttc gccctgacgc acgttcataa tggtcacgcc aggcggacag atagactgca
     1021 cttccgccaa cagttgacgc ttcacgcgct gttcaaggag agactctaat tcataagagg
     1081 tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt caccaggagg atttctttga
     1141 tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg acgatcggta
     1201 gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccaaga cccgctaccg
     1261 gtataactgc ttttaaattc gtcattattt tcctacctct aagtggctga tagtgcgtaa
     1321 attattgtca taggttagcc aaacggtatg gctatatacc aagcataact ttgattaaac
     1381 cttacgataa cactacacac catcagcatc tgggttactc ggattactcg gaaatccaca
     1441 tactgataat ttaatcagta cctctttccg aataatcgta gtccaacctg gtcctttttt
     1501 ctctgactcg tctgcattac tcagaaacaa acgttatgtc gtcttttttg gcatggacga
     1561 attcatactg cagagttcga tcaagacctt gcgacagcgt atacggtgcc acaaaacctg
     1621 aagaatgcac tttcgttgcg tcaaactgtg ttgttgcgca gaattttttc acgcgcacag
     1681 agctgacagc gtattttttg cccgtaattt tgctcaggat atcaaagcaa tatccaccca
     1741 gcattcctag tgggtaaggc aagtgcatag aagggatctt tttgttcagg ctttgttcaa
     1801 cttcagcaac caactggttc atgttcaggt ctggcttatc aacgtagtta taaacctcat
     1861 aacctgcggc aacattcttc agtttgtact tgataaactc aacaatgttt ccaacataag
     1921 ccatggactt atagttagtc cctgcgccca ccatcataaa cttgccgcca gcgatctgtt
     1981 tcagcaagtt atagacgtta ccgcggttgc gttcaccgaa gataacggta ggacggatga
     2041 tagttaatga acgttctgtt ggtgctttgt tataccattc acgcagcact tcctccgcct
     2101 gccacttgct tttgccgtag tggttgaaag ggtcgtgtgg atggttttcg tcagggttgt
     2161 gtttgttcaa accataaaca gcaacggaac tggtaaagat gatattttta acgccatttt
     2221 tttccatggc cgccagcaca ttgcgggtac cctgaacgtt gacatcataa tagagagaag
     2281 tagggctgac gtcatcgcgg tgttccgctg ccagtagtac aacagtgtca aaaccggcta
     2341 acgcctggtc gagtgcctgt tgatcacgaa catcaccaat ctgtgtgatt tctggataaa
     2401 agtggctctg ctgtttgtcc aggttcttga tattaaagtc agcaattgcc gtttcaagta
     2461 gtcgggttcc tacgaatccg gaagctccta tgagcaaaac gttatcgttc ataaatcact
     2521 ttagtctggt tgttacgtaa gaaacacaag ataaagatga gtaccttccc tgagtagtca
     2581 atgctgccca gccccagctt taacagttag tgtgaggatt ataatctttt agaacattat
     2641 atccagtaag tttatgaatg gtcgcaaatc tactctctcc gttccggcaa tctaaagtta
     2701 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgtcct
     2761 ttctcattca ccgcatgaac atttgcaaga gacagcagtg tttcttcttt cgccataaag
     2821 acgcctcgaa cgtctttgcg catgtcgaag ttcataatca atgcgggtcc aacagaggat
     2881 tcctgcatca ctttgatatt acgcagaaaa agatgttgcg gtttgttgtg taactccagc
     2941 gacgcacgct tcatctcaat gttagttagt gccacaaagg agacggcatt cccggcagag
     3001 atttggaggc cgcgcaattt ataagcaaga tgggtgttat ccagttgaat attattcact
     3061 cggaaatttt gcggaatcga gagatatttg cctttaatta ccccatagcc gattaacatc
     3121 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
     3181 actgtggcgt tatcaatgcc tgctgtctta ctgaaatccg gcgtgatatt gcgggctttg
     3241 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
     3301 cccgtgatat ttgccacgac aaagtttttc actgcctggt cttccgggta gttattatca
     3361 taagtactcc ccgcaaggcc tatgccaatg ccccagttga ttttgccatt agtacagttg
     3421 atgcgctcga tgacatggtc agagatcaaa atatcactgt cgttaattgc cacgttccat
     3481 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg tgatgttggc accgataatc
     3541 tggttatgaa atccctggcg taagatggcg taattagcgt ggctaacggt caggttatcg
     3601 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccga tataaatctg cgttaccggg
     3661 ccaaagccgc tcatcgccag ccctttgatg gtgcaatcag aaccacgcac atccagggtg
     3721 atgttatgca tactgccgcc atcctcccct gtcacctggc tgccgtcctg taagacaaat
     3781 cttcctctgc cgttgccgcg caggcttcca aggatgtgta acgttttacc tgcaggaatg
     3841 aagatgccgg tgttgatatt gtcgcaaacc aatccggcgg gcacgacgac cgtttgccct
     3901 tcgctgaagg cttgtttaaa tgaggcgatc cagtcgtgag ggttgtagtc gttaatgttt
     3961 acgctttgtc gggcggatag tgcgcgagcg aaaggggtat ggaggaaggc aagcgcggag
     4021 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatt gcatacgttc tcctctataa
     4081 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
     4141 tttttcgcga gcacgtttga cgacaggagc cagttcgtcg gtgtccagtt ggctaaacgc
     4201 cgccagtcgt tgcgccagtg cgcgagcatc gttctcaggc accagccagc cggatttatc
     4261 ggcctcgacc agttccggta ttccgctatg cagagtagaa accaccggaa tgccgaccgc
     4321 catcgcctcc atcagcgcca ccggaatgcc ttccatatcg ccatccgcac ctgtaaccga
     4381 tggcaacagg aagacatccg cgtcgtcgag catcgctttc acttcatggc tcggtttaaa
     4441 gcccggcatc tccaccacat cttccaattg atattgttcg atgagcgtgc gcaggcgtcg
     4501 ttcccacggg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
     4561 ccggcaggct tcgatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacggaaat
     4621 gatttccagc ggcgttgcgg gtgctttcac cggacgcggg ctaaaacgtg tcatgtccac
     4681 gcccatgcgc gatacggcaa ttttttccct cgggcagccc attttttgca gccttccggc
     4741 ccacagattg cttattggta acatcaggtc gccacggcga aacagttgtt gatattcggg
     4801 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
     4861 tttgccgcga atgacaccca gttcgcggag ttttgctgcg gttacccccg cagggccaaa
     4921 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tggccgcaaa tggcagacaa
     4981 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcctgcc aggtattttt
     5041 acgatgaatg ccgcgcaagg tctgactggc tcggtggcgc agcttcgcca ctttgcccgt
     5101 aggttcgtcc tgtaaccagc gggttctggc ggcaaggttg tatttcgtcc atgccgcgtg
     5161 ggtgttctgg gtgtcgcctt tttgcagcgc gacaatctcc acctcaaatc ccatatcaat
     5221 aaacgcggta atttgattga ggacaaaggt ttccgacgac agcggaaatt tcagtaaaaa
     5281 gaagccgacc ttcatttcac ctccccgatg cgttcaagca cagattgcac catctgcatt
     5341 cccgtctgac gttcgcgact aacggcttcg ttaagtcgtg tgttcagcgc cggaagctgg
     5401 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcta ataaatgacg gatatcaatt
     5461 gccatctccg gtagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
     5521 gcaatcgccg gagtggcaaa gttcattgag ataatggcgg agtgcagacg cgtaccaacg
     5581 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
     5641 acgtggtaac gggcaggatc gctgatgtgc tggcgcaaat tgagcgccac catgcggtcg
     5701 tctttattat agctgtcgat gcccgtacag gtggagagcg caatcacctg atacccttca
     5761 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
     5821 agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccaccgtttt ctgttgtgcg
     5881 gcaacgtcca gccagtgttg aacggcatag ctggcggtga aatcttcggt gtggtgatcg
     5941 accagccacg cggtatcgac gccatgttca acttttgcgg tggtgatatt gctgcgtttc
     6001 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcat cgcagtgacc aaaaacgtaa
     6061 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
     6121 agcggctttt tcgccataaa tgtgcaaagt gcgtgttcaa actgcggcac gccatagaga
     6181 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgaaag caggcgcacg
     6241 aagtcagtga atccctgggc gatggcgata ttacgcagct tgccagtgtc ggtgacgcgt
     6301 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaacgcg cccgacaacg
     6361 cccgccgcgc tgttgtgttg tttcatttgc aggaacagcg gatcgcccat caccgggcgg
     6421 ttgagcagcc aagaagaact gaccggatag cggctcatca cgtccacttc ggcatgtgga
     6481 ttgaggatgt tgatggcatc aagtaagccg cgcaggatgg cgctgtcgcc acgattgccg
     6541 caagtgtggt tgcccagaat cagtaatttc attttttcct cataaatttg atgccgggtg
     6601 aggttagttg ttgttttcgt tgccggaacc gaaaacagcc attcaccccg cccgtaaaag
     6661 cattttcatt ttttcgctgc gacaaaactg acgcttcact tccaccacca gcggatggcg
     6721 cgacagcaca atcatcacca caaacgccag cacgcctgca gctatttgca ccgccagcag
     6781 catccccagc gccagttgcc ctttcagcac aatgcccagt gcataactga ccaccagcgt
     6841 cggcagcgag agataaaacg gcagccataa actcaggatg tactggcgat aactggaacc
     6901 aagaaccggt ttaatcatca cgaagtaact gagaatggtg ttgataatct gcaccagcag
     6961 gaaaccgagc gtgacgccga ttgcgcccgc catctgccca ccaataacaa tcgccggaat
     7021 aaacagaaat gttttgaata cgttgaattt aaagctgata tcgacccgcg ctttcgccat
     7081 cagtagcgaa ccaatcgggt tacccactga gcgcagtagc cccaccacac acagcaattg
     7141 cagcaccgga ataatgctgt tccacttttc gccaaatacc agcggtacaa agttattcga
     7201 caccaccatc agccccagca gcgccggaaa gttgataatc cccaccaccg acagcagctt
     7261 gtagaagtta atgcgcaact tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa
     7321 cacgcgggtg atgatcgggt tcagcttcat cggcggtacg acggccacgt tgtacgccag
     7381 gttgtatccc cctgccacgc ccgcgccgag aatacgcgcc agcaccagcg ttgaaaggtt
     7441 ggtgttaaga tagttgatga tgctgtccgc cgtcagccac gccccaaaac gtaagttcgg
     7501 tgccaccgac gccagcgaga aatgcagacc ggggcgataa attttgcgac caaagtagcc
     7561 aaacagcagc gttcttaccg cactattgac cagataaccg aggatcgcgg tcatcgccag
     7621 cggccagaaa tgggcgctaa ccaccgtaaa agtgaagccc gccagtaccg cgctggtttc
     7681 gatcatgccg attttgttga actccagctc cttctgcatc aacgcgcgga actgttgccc
     7741 gtgggggatt accacaaacg ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt
     7801 attcagcacg tcgccgatga gatcactcaa caaaaacacc accacgcaca ccacgatccc
     7861 cagcccgacg ttcagccagt acaacgtggt gagttcaagg tgactgattt cttttcgctg
     7921 aataatcgag ttagcgatac cgaagtcaga aagcgtatct gccagcgcga taatcaccag
     7981 cgacacggta agcaggccga actggtggtt gtcgataatc cgcgccagca cggtcatctg
     8041 caccagcccg aggccgatga tgatcaccgt ggcaatcgcc gaccacttcg cgccgctgat
     8101 ggttttttca cgtaagctca tatcaatatg ccgctttgtt aacgaagcct ttgaataccg
     8161 tcaggaaaac gattttgata tcgaaccaga cgctccattc gcggatgtac tcaaggtcga
     8221 actcgacgcg tttttccatt ttctccagcg tgtcggtttc gccgcgccag ccattaatct
     8281 gcgcccagcc ggtaatgccc ggtttcactt tgtggcgcag catgtagcct tcaatgagct
     8341 ggcgatactg ttcgttatgc gctaccgcgt gcggacgcgg accgacaatc gacatcccgc
     8401 cggtcagcac attgataaac tgcggcagtt catccagcga ggtgcggcgc agaaagttcc
     8461 ccactttagt gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca
     8521 tcactttcat ggaacggaac ttccacactt tgatcggctt gccatccatg ccgtagcgag
     8581 tctggcggaa aataaccggc cctggtgaac tgagtttcac cgccagcgca atacagcaca
     8641 gcaccgggga gatcagcagc aagatcagcg tcgccagcac aatgtcttcc gcacgtttta
     8701 gcaggcggtt aaccccggaa agcggcgtgt catacagcgg caccaccgga acgccgttca
     8761 tctcttcgag acgtgaatgg agaatattga aggtaaagac atcggggatc agcagcaccg
     8821 aacaggtggt gtccgccagt tgatggacca gttttttcac tcgcgcgccg tcgcacattt
     8881 gcatcgcgat atagacgtta tgaatcttac ccgctttagc atcctcgacc agctgttgca
     8941 gattgcccgc ccagtcgtta gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca
     9001 cttcaaaccc taaccacggc tgattacgga agctctccat cagcatttgc ccggcggcta
     9061 aatcccccgc caccgcgacc atgcgcttgt tatagccatg attacgcagc cagcccgccc
     9121 caatgcgaat acatgaacgg caaaccacca gtccgatact ggtcagccca taccacgcca
     9181 gccagatttt cagttgcgtg tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa
     9241 tcacgcttaa ggtccagttt tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc
     9301 gccatgagcg ataaaaatcg gtgatgccgc ccagcatctg gaacaccacc agcgtaatca
     9361 acgccaccaa caggtgcatg tagaggaatg acagtccgct cacttcgcaa accagccata
     9421 gtccggcaaa catgatggtg atatctgaaa agcgttgcac catagagatt aacgatgcat
     9481 tggttttcgc tcgctcgcgc ttttttagat ttgtcatcgt tgttcctgtt ctattgtcgc
     9541 ctcgcccctc accccggccc tctccccaaa ggggcgaggg ggagaagaaa gttccgtgct
     9601 cacttctctc cgcaggcagt agagcgtcag ggtgaggggg aaagtaatga cattactcgt
     9661 tcagcaacgt cagcagagtt cgcgttcgcg cttccatcag cggtacatca ccgcgcgatt
     9721 ccacattcaa ccgcaccacc ggttcggtgt tggaggagcg caggttaaag cgccagtcgg
     9781 caaaggtcat gctgatgcca tcggtgcgat ccaccgccag cgcctcgcgg ctaaaatgct
     9841 gttcgacgcg gttaatcgcc tcaacgggtt gcgccagttt gctgttgatc tcaccgcttg
     9901 ccgggaatgc cgccatccgg tcgtgcacca gttcgcccag cgtttttcct ttcaggcaca
     9961 ccagttcggc taccagcagc cacgggatca tgccgctgtc gcagtaagcg aaatcacgga
    10021 agtaatggtg agcgctcatt tcgccaccgt agatggcgtc ttccttgcgc atccgttctt
    10081 taataaaagc gtgtccggtt ttcgacatta ccggggtgcc gcctgcggca gtcaccacat
    10141 caatagtgtt ccaggagaga cgcggatcat ggatgatctt cgcgccggga tttttttcga
    10201 ggaacgcttc tgccagcagg ccgacaatgt agtagccctc gataaactgc cctttttcgt
    10261 caaacaggaa gcagcggtca aaatcgccat caaaggcaat gcccatatcc gcgccgtgtt
    10321 tgatgaccgc attgcgggtg tcgtcgcgac attccggcag cagcgggtta ggaataccgt
    10381 tggggaaatt gccgtccggt gtgttgtgta ctttgattaa ttccacgggc gcgccgaggg
    10441 ctttaaagcg ggcttcaatg gcgtccacca ccggacccgc tgcgccattc ccggagttaa
    10501 tcaccagctt gagcggcgtg aggtttttga cattgatata accgaacagg tgatcaacgt
    10561 aagcgtcacg caggttgatt tgctgatagc ggccgcgttt ggtttcatcg acgggaggaa
    10621 agtcgttggc ttcagccaga cgctggacgt cgcgcagtcc ggtatcaccg ctgatcgggc
    10681 gagccccttc gcgcaccagt ttcatgccgt tataatccat cgggttatgg ctggcggtaa
    10741 cttcaatgcc gccatccacg ccgagatgga acgtggcgaa atagatctct tctgtgccgg
    10801 acatgccaat atccagcaca tcgacgcccg catcctgtaa acctctcgcc agcgccagtt
    10861 ttaaggtttc gctggtgagg cggacatcac cgcctaacac aatggttttc ggtttgagaa
    10921 attcgccata ggcgcgacca atgcgccagg cgatatcttc attcagttct tcgcctagtt
    10981 ttccgcgaat atcataggct ttaaagcagg ttaatttttt catatcgtta ccctttttca
    11041 ggcaatagtt ggccctgacc gaagcggcca attttatttt tgtcgttatt tacaccgcgg
    11101 tttcgcattc attgcctgat gcgacgctgc cgcgtcttat caggactaca aacccgaacc
    11161 gtaggtcgga taaggcgttc acgccgcatc cgacaaacag ctcctgatgc gacgtttaca
    11221 cccgtccgta gcgatccgcg aagcgcacca catcatcctc ttcgagataa gagccggagc
    11281 gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcagtgc gtcgccccca
    11341 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccgtca atggtgactt
    11401 ttgccgttcc cgcgaccacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    11461 agccttcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    11521 agtcatattt gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccgt
    11581 cggctttgat ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca
    11641 gcaccgcatc tttggtctgc accaccacca gatctttcac cccgacggtg gttacgaggc
    11701 cagattcggc gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccatggcaaa
    11761 cgttgccctc ggcggtgtgg gcactgatct cccataaaga ggaccaggaa ccgacatcgc
    11821 tccagcccgc atccatcggc accacaacgg catctgccgt gcgttccatg accgcgtaat
    11881 ctaccgactc ttccggacag gcgagaaacg cctcttcatc cacacgaata aaatcgagat
    11941 ccggatcgac ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatactttt
    12001 tcagttcttc gagataacgt ccggcacgga acaggaacat accgctgttc cagtaatatt
    12061 cgccacttgc cacataggcc tgcgcggttt ccagattcgg tttttcgaca aactgcgcca
    12121 cttcaaaggc caccgtatcc tgctcacccg ccgacacttc accgcgacga atatagccat
    12181 atccggtttc aggtagatcc ggcacaatgc cgaaggtcac cagcttaccc gcttcggcat
    12241 acggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg
    12301 ccgccagcac cagcattaat gggtcgttct ccgggctatg acgttttgcc gccagcgccg
    12361 ccagcgcaat ggccggtgca gtgttacgcc ctgccggttc gagaataatg ttctcggtga
    12421 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    12481 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    12541 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    12601 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    12661 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagcgtgc gttcggcata
    12721 ttcacgtgcc accgtgttgt gtttgggcag caggaacgcc tgacgaatcc ctgccaccag
    12781 cgcctcgacc gattccggtt ccacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    12841 ccccagttcg gtgtgggctt ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt
    12901 ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca
    12961 atcccccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggaa aaaattgcat
    13021 gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc
    13081 gacaatggca aaaataagcg gttcatcgcg caggcgatcg gcagcttcaa taacgttttc
    13141 cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag
    13201 gccaagctgg ttacgaaggg catcaacatc ggcgtctgcg acatgctgaa aacgggcaat
    13261 ttctgaccag ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatagcttt
    13321 attcatcatc gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc
    13381 gaacgccgtt gccagctgtg ccactttgcc gcctttacct tttccggcaa gccccagccc
    13441 cagcatggcg tccacttcgt aatcctgaat atggagcacc gtgcgcgcac cagacagttt
    13501 cgccagcagg cgcattcctg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc
    13561 cggcttccag cgacgttgcg ccatcagtgg aaagaaactg ctgacggcaa aactgccgag
    13621 atgcaacagg cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac
    13681 cgtggcggcc ccctcttccc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca
    13741 ctgcgggtag taaggcggtg cggtaataac ccgcacctca tgaccttgtg ctgccagcca
    13801 ttccaccatt tctccggtgt atttgccgat gccggttaac tccggcgagt agttaatgcc
    13861 gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg
    13921 cggctgttag cgtgaacatt gtcactggcg agcagtgcgt ccggcgtcag ccagcggtaa
    13981 tcgtcatgct gctcatccgg cagtaacagt tcttcttccg ctactctgaa gcgaaaaccg
    14041 agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc
    14101 cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc
    14161 agccgctcaa atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac
    14221 cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcgcc gcgactgttc
    14281 tcgacaataa agtcgagaga gacaagcggt gtggagcgca ctaccgtggc aaagtcttcc
    14341 tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag
    14401 tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg
    14461 tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa actacccgac
    14521 ctttgtaacc caccactttg gcgatggttt gcgccagctc acggatagtg cagtcaacgc
    14581 ccgtgccgac gttaatgtgc gacagcatcg gctgggtgtt ctcctgccag acttcgtgcg
    14641 ccagctccat cacatgaatg ctcgccgctg ccatatcatc aacgtgtaga aattcgcgca
    14701 tcggcgtacc gctgccccac accaccacgt ccggcgcatt ctgtgccgtc gcctcatgga
    14761 agcgacgcag caatgctggg atcacatgcg aattactcgg gtggaagttg tcgtgtggcc
    14821 cgtacaggtt ggttggcatg actgagcggt aatcccgtcc gtactggcgg ttgtatgatt
    14881 cacacagttt gatcccggcg attttggcaa tggcataagg ctcgttggtc ggctccagcg
    14941 tgccctgcaa caactcgctt tctgccatcg gctgttttgc cagtttcggg tagatgcagg
    15001 acgatccgag aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    15061 tctcaatcat catgttctgg tagatgaaat ccgccggata cgtgttgttg gcaacaatgc
    15121 cgcccacttt cgccgccgcc agatagacct ggtcaatacg ttcgctggca aagaaatcat
    15181 gcaccgcgcg gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat
    15241 caccgcgctg ttcgagctgc cgcctgatgg cagaaccgac catcccgcga tgaccagcga
    15301 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    15361 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    15421 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    15481 gccgagcagt gtttcaactt cagccggacg gaagtaacgc gggtcaacgg cgataatcac
    15541 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    15601 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgccgccg ccatttccac
    15661 gaactgacgc acggagtact gaacgccggt agcaataacg aaatcttccg gttgttcctg
    15721 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    15781 atccatattg ccgagataca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    15841 ggtgattttg cgggtaacga aagtctcgcc gcggcgcggg gattcatggt tgaagagaat
    15901 accgttacag gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    15961 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtctctt tctgcggaat
    16021 ttcctgcacc agaccataca gttcagaggt ggaagcctga taaaaacggg ttttcttttc
    16081 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    16141 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac
    16201 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    16261 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    16321 ctcggtgttg aatgacgatg cacgacgctt aataccatgc acctcgtaac ctttttccag
    16381 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt
    16441 tgacatgtct tattcctctg tattttttga atttattcag tttcaacgcg ttcgcgtatc
    16501 accactgcgg gattcccccg gcaaaccaca tttgccggaa gcgatttaaa aacactgctt
    16561 cgcgccccca cgacggtgcc gtcgccgatt gtgacgcctg gggcaacaaa gacatcggtt
    16621 gccagccagc atttctcgcc aatcacaata ggcgtggcgt taatggtgaa atgttgactt
    16681 gcatggtcgt ggctaccggt gcataaataa cttttttgcg atatcaccga atgtgcgcca
    16741 atggttattt caccgagggt atataaattg acgtcatcgc cgacccacgc gtaatcacct
    16801 aaggttaatt tccacggata ggtaattttt actgacggcc gaataactac gttttttcct
    16861 atttttgcgc cgaataaacg taataaaaat gcccgccagc gatacaatac ttgtggcgac
    16921 caggcaaata atgttgcctg tactgcccac cacaattgca ctttaatagc gttgccaccc
    16981 cggaaccctt tcggcaccga gaatccactt aaatcctgca tcgttttccc ttatattcag
    17041 cttttgttat ataaggcttt cgtctttgag gtagtacgtt ggcgtaaatg ccaggataat
    17101 tctgcccaga agccaggcac atgtaatatc tgccgttgga cttttttggc gtcagcacac
    17161 aattccatat tattggtggt tgatacccca cccatagaaa attcagacac caggccattg
    17221 agttttttaa atgcataacc tgctttatac attttagctg ccagcgcgta atcagaagaa
    17281 actttatatt ccaggtcata acgccatttt ttcaagccgg atacagggaa aaatatcgcc
    17341 tgatgactgg cgggcaggct gtgataaata taccagcccg gtttggcgct acgtttaatt
    17401 ttatgcccgt cgccaaaatc cagcagcgca tcgccggtga tcatcatgtt atctttttgc
    17461 atttttaact tacggacaaa atttgcggca tcctgatgaa aaatatcgcc cgaattgaga
    17521 aacaacgcga acttgccttg tgccatcgta atgcctttgt tcatggcgtc gtagataccg
    17581 ttatctggct cgctgacaaa gcgtaggtta tagataccat tgagattttc cagatactca
    17641 cgggtgccgt cattggaacc gccatcgaca acaatccatt cgaagctgat atcttccgcc
    17701 tgcgccagat gcgccagcga ggcatgtgtt ttgactatcc cttcgaggtt acgaaacgcg
    17761 acagtgatta tgctaagcaa catgttctta ttcttacctc gtaatattta acgcttttcg
    17821 caaaataaac ggacagacga ttaaaaatgc atattccggg ctaaatatcg aaccggtaaa
    17881 aaacagcgac accggagtaa aaagatataa ttgcacgcga aaattgcggt tatcaccaaa
    17941 agcgttgatc atcattttta tcactttccc catgtaccac agcgataaaa acactgcgaa
    18001 ccaggaaaaa taaataatca gcagatacaa accattgtct atggtttttc cgacatccgc
    18061 accgttaaat attccgaatg atgcgacgta ttcataaagt gagccaaatc tgactacacc
    18121 gtcaatatgg gtcaaagaat aaccgaccat caccagcgga ccgacaatac gataatacga
    18181 tgacgatcct tccgtaccta aatcgcccag acgggtggaa atataaggaa acgcgattac
    18241 cacaccaacc tggaatacag ccagagaaat caatgccaac ggtaactttt tcttaatcgc
    18301 ctctttattc agatattgaa atgcccactc cagcaaataa aacaggataa aggtcataac
    18361 ccctgaaaac gatccggata atattatccc tgcgagaatc atagcatcgg ttttaggcgt
    18421 tttgatacca aactgtttga tgctgagcca aattgagatt aatgccagag cgaaaaatgc
    18481 cggttcgaaa taaagtgctg tcgtgcgctt gccgccgaat ttaatgaaat tcagtacata
    18541 gctgttgctg taaatcagat atttcgaaat cgactccata atactgctgc caccggtgag
    18601 gataatttgc gccatctcca ccgccgccag cgccaccacc agcccgacca ccagataaaa
    18661 gaaacgtaat atcttgcgat ggttgtgtgg cgaaatcgtt ttaaagcgaa tactccacac
    18721 catgccaata atgatcacaa tatagacaaa cagcatggtt gaagtgacgt atttactggc
    18781 atccaacgac tgaccaaaca gatagttgaa cgccgtgagt cccgcaccaa tccctaaggc
    18841 aatcatcaat tttttaacgc tgatgcgttc taaaaacagc agtaacaaga caggtaaaaa
    18901 agtgacgata gtgatgggga aactttcacc aagctgggcg attttgacgt taaccagcag
    18961 gtagatcagc ggcagcagca ggtagctaca gattctgata gaagttgaca tactcctcca
    19021 gcatctgttg tccactgtag gcggcgcggc tgcgttggct gaactcagcc agcgtggtac
    19081 caaatatcgc ctgcgcgatt tccggtttgc ttaactgcac cagttgcagc acctcttctt
    19141 cactgacggt tttaccaccg gatttttgca gcacttcccg cgccgcatcg ctatgggtgg
    19201 cgatcaccgg cacgccaatc gatagcgcct cacacaaaat cagcgggtag ttatcgacgc
    19261 gagaactgaa caccagcgca tccatttgat tgagcgcgct catcaacttg cgcttgtcag
    19321 tttcaaagcc gtgattaacc acgttgccag cggtgaacgg cgagaactta ccaaaggtat
    19381 gcagttcaat tttgtcgccc agcgccatca tctcacgcac cagttgctgg ttagtcttgc
    19441 cgtcgtaacg caggtcatgc gccaccaccg cgattttcgg cttgccctgg gtttcgcgca
    19501 ccggaggcag atccgccaga atcgcttcgg ttgccatatc aataccatta ttgataatcc
    19561 ggcaacgccc tggaccgtac aggctattga aagcgtcagc cacatgctgg ctgggggaaa
    19621 taaactgaca gcccagcgcc agcatctcac ggaataactg gcgtttgccc gccaccagct
    19681 ggtgcgcgcg atcaatcttc accggcggat agttatttaa ggtcgggcat ttctggcagc
    19741 ccgttttcca gccttcacaa ccgtcggtaa aggcgcagcg tccggtaacg ctccagtggt
    19801 cgtgcagcgt ccagaccaga gtgacgtccg gtttatggtt tttcaccttt tcgcaaaagc
    19861 gcaccacgct cttaagattt agccagtagc tgtgcagcac atgaaaatgc aggaccaccg
    19921 gacccggtgt acgagtaatg gtgcgatata actcattgaa attgccaaac agatcgcgat
    19981 taaacagacg aaacaggaca atattcgcca ttgcagtcat ccgcggcgta tgtttgatga
    20041 cctgcggata gttttgatgg ctgacgctct ctttgccgcc tttgccgtaa ccgtagacaa
    20101 aatgtgacgc tagcccctgt tgcagcgcac gctggtggag atctaacgcc acacctgctg
    20161 ccccgccttc cgccagtcgc acattaaatt gcaaaatatt catttaatta ccttcactcg
    20221 cgctttttct cccaccacca gcgcgttgtc cgggacagag tcgagcacca cgctgcccgc
    20281 gcccacggtg acgttgttac caagcgtgat atcaccaaga ataataacgt tggcaccgag
    20341 ttcgacgccg ttgccaatat gtggacatgc catgttatcg gcaccacgat tgccgatagt
    20401 gacgccgtgg cgaatggtga aatcatcccc cgccaccacg tttttattga tcactacggc
    20461 gtaaccgtga tggatagtaa agcggcggcc aatggtcgcg gcagcctgga tttcataacc
    20521 gaaaaagcat tcggtgataa tgcgatacag caccagcaga ggggccgccc aaatattgtt
    20581 gaggacgttc tttttgcgcc acaccgaaca aaaatgagcg acgcgatacg ctagaaccat
    20641 gcagcacgga cgtaaactcc agctgttggc gcgcagatct tccagcatct ttagcgcccc
    20701 cgaataccat cagccagacg tttgccgtta cgcaccgaca gcagcgtcag caaagtgcgc
    20761 caggtcatac gtttattgcg gatctggtag agggtaaaca gctgatattt tttactggca
    20821 cggtcaaatt tgtctttgtg cttgcggtaa aagtggaagt atccggagaa ttttttcggt
    20881 gacgaggtga tctgcatttc accgtgattg atatgcagga tctgcgtcgc ctcttccact
    20941 ttccacggtt cgccatactc caccaccatc cgcaggaaga tgtcgtaatc ctgtgccgct
    21001 tttagttcgg tatcgaacaa acaatctttg aaacgccatg cccaggtaaa gacctgattg
    21061 ccaatgatat tgcgtttgta gaacaggcgg cgtgaatacg gcgatttggg atacagcggc
    21121 aagcttgccg gttgggaata gacttcgccc tggcaaacgt agtcgttagc gtacaaaaag
    21181 gcgtgtgtga ccagttgctg tttatgggcg aggaagacgc tcagacggtt gggcgtccat
    21241 tcatcatcgt catcgatccc ggtaatgtat tccccttgcg ccagcataat cgcctggtta
    21301 cgtaccgcgc acgccccgct gttaatgtcg ttgtgaatgt aggtgatacg cggatcgttg
    21361 agggcgctga cgtactgttg cagttgttcc caggaggtgg agcaatcatc cacgatgatc
    21421 atctcccagt tactgtagtc ctggcgcagg accgatttta tcgcccgaat cgccagttgt
    21481 tggcggttcc aggtcggcat atagattgag atcagcggat tgtctttcat aggttgttct
    21541 cccggcttat gagctggttt gcttgccgga tgcggcgtga acgccttatc cggcctacgg
    21601 ggcggtgcga atgcaggcgt cgttatttcg catccgactt atattcgtat tcgtaatagc
    21661 cataatcctg atacgcgctg gcgcggcgga agatggagtt cagaatcacc cctttcaccg
    21721 gaataccgtt ttgctcaaag cggctcagac tggtttccac ttctttcaat gtgttgaccg
    21781 cataacgcgc caccattaat gtggttccga catgacgacc aacaattgcc gcatcggtca
    21841 ctgccagaat cggcggcgta tcaatcaaca ccaggtcata gtttttactc gcccagttca
    21901 ccagttcggc aaagcgttcg ctcatcaaca gttcagaagg atttggcggc acctgaccgc
    21961 gcgggatcag gtcaaatttg gcaatagagg tcggtttagc tgcggtagta atatcgccct
    22021 gaccaatcag aatttccgac aggccattga cgttattggt gcccaacagc tcgtgggtgt
    22081 agcctttgcg catatcgcag tcgatcaaca acacgcgttt attggtctgg ctgatcaccg
    22141 ccgccaggtt ggcgcagaca aaggttttac cgattgacgg gctaaccccg gtcatcatca
    22201 acacattgtt ctgcgcctgc atcatcgcga agtgcagact ggtacgcaga ctgcggatgg
    22261 cttcaatcgc cagatcggtt ggattcccca ccgccagtag ctggctctgt ttatagcgtt
    22321 taatcccttt gatggttttg acgctatcgc gcgctttctg ccattccgac aacgggatgc
    22381 tggcatagac gctgataccg tgttcttcca gcacctgcgg gctttcgatg ccgcgattaa
    22441 acaacgagcg cagcagcaca cccacgatag agagcatcag gccaagaata atcgccccaa
    22501 ggataatcag ccctttcttc ggttttagca caccaggctg agtgattgcc gggtcaacaa
    22561 tgcgcacatc gccgacggtg ctggcttcgg tgattttcag ctcctgctct ttattcagca
    22621 gttgcatata gacctgctga ccagactcga catcgcgggt cagacggaca atctcctgct
    22681 gggttttcgg catcgccgtt acgcgaccgt taagtttggc tttttcgtct tccagcgcct
    22741 gacgtttctc cagcagcgtg cggtacgccg ggtgaacttt ggtgtacagc ttggagattt
    22801 ccgcctcttt aaaggtcagt tcgttcaact gggcgtcgat gttcaccatc gaatcgagca
    22861 ccgctttcgc ttccagcggc agatcaacag aatctttatc ctgacggaag gcattcagtt
    22921 tgttttcggc aacatccaag cggctacgta cttccggtaa ctgttgcgcg aggaaggcga
    22981 ggcttttcga cgcttccgcc gatttgcgct caatattttg ttcctgatag ttacgggcga
    23041 tgctgttaag aatgtcgcgg atctgttcac gatcttcacc ggtataagtc aggctcaaaa
    23101 cgccagcgtc tttgccgttc tccgttaccg tcaggctgtt ttgcagttgg ttgatcatcc
    23161 ccagcgtgga gtatttggtg acggtaaact cactgcccgg gctggcgtga atggcttcaa
    23221 ccatcagcgt gacgccttct tttttcagca tctggcccgc ttgcccccgg gcgctaaagc
    23281 cgccgtcgct gctcagggtg tagtttttgt tgtccagcac attaagcgta aacacctgat
    23341 ccgccatctc tttcgggcgg ttaaaggtgg tcactttcac cgtctcgttc tgacgtccca
    23401 tcaaacgatc ccagcccgca ccgaaaatcg ggaacgtgtt tttgctcact gcaatatcga
    23461 ggtcgagatc gtccaccgtt ttaccaagca ccagacgcga acgaatcaac tggatctcgg
    23521 cgtccgatgc aggcggtttg ttggctaatg ccgagccaat gtcctgcact aacgaattgc
    23581 cgctgttttg ttcgatttgt accagtgcgt cggcactata aatcggcgtg gcgaagaaag
    23641 tgtaaaccac agcacagagg gcaaatacag cggtgatgcc aatcacccac cagcgcgctt
    23701 caatgacggt gccgaccagg cgaccaatat cgatttcatc actgcccgtt accggagcgg
    23761 catgttgttt tactttttct gtcattctta tacctgctct gcgttcaatg cctgcgccca
    23821 ctggcgggca gaccgttcaa gtaatgtgta caccgctgca aacgtttccc ggcttttgcg
    23881 atacggatcg gggatttcac attcgttatc ccagtgacca aacagcatca ctttgccgcg
    23941 catctcaggt gccatctcgc ataagcgttc gatatggcgc ttttccatgg tcaaaatcag
    24001 gtcatagttg cgacacaggc ggcggctgat ttgccgtgca cagtgacctt ccagagacag
    24061 ttgatgttct gcggcgacgc tgatagcggt aggatcagcg ctcttaccga ccagcgcgcc
    24121 gagtccagcg gactccactt tcagctccgg gtgataacgt tgcagtaagc gttccgccgt
    24181 cggggaacgg caaatattgc cgacacagac aactaagatg ttgttaaaca tgacgattac
    24241 cagttatgaa tgtcgctggc tgtatccgtc atgtaacgga caccgctaat agttggcagc
    24301 aactgattga tcagacggtt ccagcgggaa accggggcgg tggtgacata caccacgtca
    24361 taaggttgca ggcggaattc tgtcgccatc accagcgacg tcgcatcgga catatccagc
    24421 tggtagatat tggcaatctt gccgttacgc ccgccctcgc ctttcagcgg acgaatgaca
    24481 aagatgccgc tggcgttgga ggtggtcatg tcgatacctt cggcattgcc cagggcttca
    24541 gtcagggtca tgccgctaaa gtccattttc agggtgctct gtttcttcac ttcacccatc
    24601 acaaacactt tcagatcatc attacgcggc acgtagagaa tatcgccggg gtaaagcagt
    24661 cggttctgat tgaggtcgcc gttttgcatc agcgcctgca aagaaatgcg ttcttcacga
    24721 ccattgtgtg ttagcaccac gttgcgccag tcagcggtgt cggtcaggcc acctgcggcg
    24781 ttgatggcgt cgagaatggt cagtggtacg ttggtgatcg cctgttgacc ggatttattc
    24841 acctgacctg agatataggc cttttgcgag cggaaggcgg cgatattaac atccacctgc
    24901 gggtcagcga tgtacgtcgc taagcgcccg gtaatatcac tgcggatttc agcgagcgtt
    24961 ttcccgacta cgtggacctt gccgatatac gggtaaaaca tagtgccgtc aggctgtacc
    25021 cagttgccgg tgtcgctgga gctgcggtac tgaccggctg gcgtggtgag ttccgggtga
    25081 tcccagacgg tgacattcag aacgtcgccc ggcccgacgc gatactggta attcgcgatc
    25141 tcactttcca gcgtcatatt ggggcgcgct acattcgggc gtgggcgtaa ttggtcaatc
    25201 aggcgcgggg tcagcggata aacattcacc attttgtcga gatcgaaatc agcgtcctgc
    25261 tgtttgatga cgtctttgcc catcgtcgac atattgctgc ccggaagtac tgtgcaaccg
    25321 cttatcaagg ttactgacac caataatggc atcaatttca ttttggattt catcattgtt
    25381 tatttatcac tttggcagag taattatcct gtgcactatt aatagcaatg tcgccatgca
    25441 catttacctt gcagttaatt gaatacaaat ttaactggca tcagtcctaa aaaaattgat
    25501 ttcatccgca ggctattgac agaataattc agactggtct ttcaggcatc cagacacgct
    25561 accgcccctg gctttttagc taccaataca ctgatttagc ttaatttttc aaaccctctc
    25621 tgcatacaat cgttgatgag aaagggttat tgcggaaatt aacttccgaa tataaggtga
    25681 cattatggta attgaatatt ggctctccaa taatgcagga ggaagtgtta cagctaacgg
    25741 aatagcaggc aaggtaacaa ttcggcaatt ggctattttt aagaattata ttaagtgtca
    25801 ttcaatatgg tttttaggag tttctttagg ttgacaatat ttaatattgc gcctccacat
    25861 gcgatatttc ttaaataatg ttttattatt accactttta attcagggat aatgtgaggt
    25921 tattaccctc aaataatatg agataaatag tgctgcaaca ttgcattttt gccccgattt
    25981 atccatgatc gaattgtgac atttgtcata caacgaatag gttttgtact tactatggaa
    26041 tggattgccg atccgtctat ctgggccggg ttaatcacgc tgattgtgat cgaactggtc
    26101 ctcggcattg ataacctggt ctttattgcc atcctcgccg aaaaactacc gccgaagcag
    26161 cgcggccgcg cacgggttac cgggctgctg ctggcgatgt taatgcgcct gttactgctg
    26221 gcgtcaatct cctggctggt caccctgacg caaccgctgt tcagcttccg ctcgtttacc
    26281 tttagcgccc gcgacttaat catgctgttt ggtggtttct tcctgttgtt caaagccacg
    26341 atggagctga acgaacggct ggaagggaaa gacagcaata atcccacaca acgcaaaggt
    26401 gcgaagttct ggggggtagt gacacaaatt gtggtactgg acgccatctt ctcactcgac
    26461 tcagtgatta ccgccgtcgg gatggtcgac catttactgg tcatgatggc cgccgtggtt
    26521 atcgctatca gcctgatgtt gatggccagc aagccgttaa cgcaatttgt taacagtcac
    26581 ccgaccatcg ttattctctg cttaagcttc ctgttgatga ttggctttag cctggtggca
    26641 gaaggtttcg gcttcgtcat tccgaaaggc tacctgtacg ctgccattgg tttctcggtg
    26701 atgatcgagg cgctcaatca gttggctatc tttaaccggc gacgttttct ttccgctaac
    26761 cagacgctac gccagcggac taccgaagcg gtaatgcgcc tgcttagcgg gcaaaaagaa
    26821 gatgcggaac tggacgccga aaccgcgtcc atgctggtgg atcatggtaa ccagcagatc
    26881 tttaatccgc aggaacggcg gatgattgag cgggtactta atcttaacca gcgtactgtc
    26941 agcagcatta tgacgtcgcg ccacgatatt gagcatatcg atctcaacgc gccggaagac
    27001 gagatccgcc agttgctgga gcgaaatcag catacgcggc tggtggttac cgacggtgat
    27061 gacgcagaag atttgctcgg tgttgttcac gttatcgacc tgctacaaca gtcactgcgt
    27121 ggcgaaccgc tcaacctgcg ggtgttaatt cgccagccgc tggtgttccc ggaaaccttg
    27181 ccgttgttac ctgccctgga gcagttccgt aatgcccgca cgcactttgc ttttgtggtg
    27241 gatgagtttg gctcggtgga agggattgtg acattaagtg acgtcactga aaccattgcc
    27301 ggtaacttac cgaacgaagt ggaagagatc gacgcccgcc atgatattca gaagaatgcg
    27361 gacggttcct ggacggcgaa tggtcatatg ccgctggaag atctggtgca atatgtgccg
    27421 ctgccgctgg atgaaaaacg tgaatatcac accattgccg ggctgttgat ggaatattta
    27481 cagcgtattc caaaacctgg cgaagaagtt caggtggggg attatttgct taaaacgttg
    27541 caggtggaaa gccatcgcgt gcagaaggtg cagattattc cgctgcataa ggatggcgag
    27601 ttggagtacg aggtgtgatg gtatcggtca ttttacccga tagccgaaag gaataagatg
    27661 agggaaccgc gccagcggcg atttaccgcc gggagcccgg gttgccaggg tggtggcgat
    27721 tgagccaccc tggcacgttc accggcttga gcgttccata gtagtgagga acatcaagtg
    27781 aacggaacag ccttcgatct tacatattca gaatataatg gtgccgccgc gaacgcctga
    27841 tgcgacgctt tacgcgtctt atcaggccta caggacaggt cacctggtaa cctacatctt
    27901 ctccagcaac ttcttcacat ctttgccatt gcgggaatct ttattccgct cggcccagtc
    27961 attcaggcga cgtttcgctt cgtcctgtag atgtttgcgc agtaactgat ccacttgcag
    28021 actgtaattg agttgctgcc agttgccata aacccgcagc ggtactggcg tttctttgag
    28081 gaaatcaatc agtttgcttt ccccgttcca gccacccacg acccgaatat caaactgggt
    28141 gtcgcaggtt tgatctgcca gattcaacat gccttcccct gtcagcgcca gcacaggcga
    28201 ttgaccttgc atgtcgttta acgtcacgac gccatccttc aacgtcaaat cggtggtaaa
    28261 gcggtcaaga cgcgttacgt tatcgaagtt ttcagcggcc ttcacatcac caccattacg
    28321 ttctaccgct tgctgaatca tctgctggaa gttcatccct tccatgcgcg tgtcggtcat
    28381 ttcgacatgt gcctgtcctt gccagttgtg gcggaatgcg tcggcatcta tgtcagcacc
    28441 ggagaaatca ccagccagtg acatttttcc ggtcaacgaa atcggatagt taaacgcctt
    28501 cagaatggta ccaatctcaa cgttttccag ccgtggctgg aagtttatcc gcggatttat
    28561 tgatgtcgcg tccagcgtgc ccggcagtga aacctgtcca ccgttaagtt tgccctgcag
    28621 ttgagtaatt tccagcaaac ccgacttgtt ggtcatttgc gtggcaacat ctgtaaaatt
    28681 cattccgcgc cagcgcacgt tactggcctg caacaaaata tcagccgtaa agccttgcag
    28741 tccctgatag gccggttcat caatacgcga agaaatgacc gggcgcggca acgttgattg
    28801 gctctgcccc tgctgcgcgg caccgttttc accattcgcg gtttcattaa gcgggatgag
    28861 gttgtcaaga ttcagttgcg ggaattgcag tctcagctgc cattccggtt tctctgtcag
    28921 cgtgacctgt gcttgcccgc tcagcgtact gtcattggcg gtcaagctga tttggttaaa
    28981 tgaaaggcgt ttatgtgact cctgccactg ggcctggaaa ctcccctgcc cctgaatacc
    29041 ttgttttggt aaatcggcac cctgcaactg ccagttaatt tgttcaatag ccgccgttaa
    29101 atcatgcgga taatcagaag catctaccgt accgttaagg gatattgtga gatcgcgctg
    29161 atcgcgatta acccgcccgg agaactcaaa tgagccacga tgttggggat cttgttccat
    29221 ttgcaggcgg atattgcgga ttgtcacctg ctcgtcatct tcatgctgga acaccagcac
    29281 gctgtccgcc accttaagac tggatatatc aaacgaccat ccgcgatcgt ctgacagatc
    29341 cggcaaggta ttgtcgcgcg gtgcaaccgg agcgtcttca ctgcgcaccg cttccgtctg
    29401 cggcgtcagt tggatcactg ccccttttag catcacctgc ttaacgctca gttgatgact
    29461 cagtagtggt aaaagcgcca cgtccagacg catgttgtcg gcgcgaacca gtggctggct
    29521 tgcgccctgg gcggtgagag acattcgccc ggagaggata ctaagctgcg gccagacgtg
    29581 ccaacgcagt gggccgccga gctgcaattg ataaccgcta cgtgcagcaa cttgcttgac
    29641 catatagtcg cggaaatcat tcggattcac cagcaacact aacgcagata acccggccac
    29701 cagcacgacc aggagtatca tcagcgtcgt cagaaatcgt ctcatggtat cctcaatggg
    29761 cctgaattag tctttatcga ttcggctggc taccgcgccc tgctggttgc gatatttcgc
    29821 atcttcacgg cggttgtaag gtcgcgccgc cgggccggaa agcggctcaa agctcagcgc
    29881 accaattaac atgcccggac gcagcgccag cggcagctta ccggagttgt agaactccag
    29941 cacaatgcaa ccagaccagc ccggatcgat acggtgcgcg gtgacgtgca ccatcaaccc
    30001 cagacgcgcc aaagaggaac gcccgtccag ccagcccacc agatcggctg gcagcgtcac
    30061 cgactccagc gtcaccgcca gcgccagctc tcctgggtga agatagaacg cctcgccctc
    30121 gtcgagaacg atctcatcgc tcatcacgcg gtcaagcgcg gcgctcactt catctttggg
    30181 accgctcaga tcgataaacg ctgccgtgtg accacggaag gtacgaaatt tattgcccag
    30241 gcgtacatcc accgtcgcgc cgttaatacg ctccactggc ggacgtgggt tgatcgacaa
    30301 acggccttca tcaagccagg cttcaatatc tcggtcacac agacgcatgg cattttctcc
    30361 tttcgcgcat cactccctta acgccaatta cgtcaagggc ataccaggtt atcactgaac
    30421 ggtacacaat ttatcaagct tattcaaaga actgacttat tttcgctttc aatatatcga
    30481 tcgcgatgcg gtttttcccg ccgcgcggca cgataatgtc cgcatattgt ttagaaggct
    30541 caatgaattg caggaacatc gggcgcacgg ttttttgata ttgcgccatc acggaatcca
    30601 ttgaacgccc acgctcgtta acgtcacgct tgatgcggcg catcaggcag atatccagcg
    30661 gggtatcaac gaaaatggag aagttaagtt cgtcacgcaa acgcgcatcc gtcagcaaca
    30721 aaatgccttc gagaatgatg accttcttcg gctcaaccgt caccgtttct ttcatacgcg
    30781 tatgttcaac atagctgtaa accggcaggt caattgccga gccgcgtttc aacgcttgta
    30841 aatgctcaag caacaggctg tgatccatcg cgctgggatg gtcgtagttg gtcttaacgc
    30901 gttcttccat cgacagatgg ctttgatctt tgtaatagca gtcttcggga attacgccga
    30961 tgtgttcatc accgacttgc tcacgtaatt cacgataaag ggtactggca ataagactct
    31021 tgccggaagc cgatgcgcca gcgataccga taatgacgca ctgatgagac tgatcagtca
    31081 tatatttagc gacctgatta acctggatgt taggaagggg gcgacgaagc gccaaacgcg
    31141 gcaattatag ggatttcatc cgcctgatac cagtcgaata gcgctgccgc gcgctcagag
    31201 ttaattgttg acgaagaatt cccggtggca aattacgttg atcagtttta tacaaggtaa
    31261 aaaatgttat acgcagttgc gcaaattatc cgcctttacg tcactttatg agcaattcgc
    31321 atataaaatg taaaactttt gtactagcat aaacacagaa acgaatactg gcgacctggt
    31381 cttgcggata aagcggtaat gagcaaacaa tcacagcatg tattaattgc cctgccccac
    31441 ccgctgcttc acctggtcag tttaggttta gtctcgttta tctttaccct tttctcgctt
    31501 gagctttcgc agtttggcac ccaactcgcc ccactgtggt tcccgacgtc catcatgatg
    31561 gtggcgtttt atcgccatgc cgggcgcatg tggccgggaa ttgcgctgag ctgctcgctg
    31621 ggaaatatcg ccgcatccat cctgcttttt tccaccagct cgctgaacat gacctggacg
    31681 accatcaata ttgttgaagc cgtggtcggg gcagtgctgc tacgtaaatt gctgccgtgg
    31741 tataacccct tgcaaaatct ggctgactgg ctgcgtctgg cattcggcag cgccattgtt
    31801 ccgcctctgc tagggggtgt tctggttgtc ctgctgacgc ccggagacga tcctctcagg
    31861 gcatttttga tatgggtact gtcagaatcc atcggcgctc tggcactggt gccgctggga
    31921 ttgttattta agccacacta tctgctgcgc catcgcaacc cacggttact ttttgagtca
    31981 ctgctcacgt tagccatcac gctgacgtta agctggcttt ctatgttgta cctgccgtgg
    32041 ccttttactt tcattattgt gctgttgatg tggagcgccg tgcgcctgcc acgaatggaa
    32101 gcctttttga tcttccttac cacggtgatg atggtgtcgc tgatgatggc cgcggatccc
    32161 tccctgcttg ctacgccgcg tacatacctg atgagccata tgccgtggct accgtttttg
    32221 ctgatcctgc tgcccgccaa catcatgacg atggtgatgt atgcctttcg tgcggaacgc
    32281 aaacacattt ccgaaagcga aacccgtttt cggaacgcca tggaatattc cgctatcggt
    32341 atggcgttag tgggcaccga gggacaatgg ctgcaaacca acaaagcgct ctgccagttt
    32401 ctcgggtaca gtcaggaaga gctgcgcgga ctcacctttc agcaactgac ctggccggag
    32461 gatctcaata aagatctcca acaggttgaa aagctgataa gcggtgaaat aaacacctat
    32521 tcaatggaaa aacgctacta caaccgcaat ggcgatgttg tctgggcgtt gcttgccgtc
    32581 tcactggtgc gccacacgga tggcacgccg ctctatttta tcgctcagat tgaagacatt
    32641 aacgagctaa aacgcaccga acaggtaaat cagcaactga tggagcgcat cacgctggct
    32701 aacgaagcgg gcgggattgg catctgggag tgggagctga agccgaatat ttttagctgg
    32761 gataagcgga tgttcgagct gtatgaaatt cctccgcata tcaaaccgaa ctggcaggtg
    32821 tggtacgagt gcgtgctgcc ggaagatcgc cagcatgccg aaaaagtgat tcgtgattcg
    32881 ttgcaatcac gctcgccctt taaactggaa tttcgcattg ccgtgaaaga tggcatccgc
    32941 catatccgcg ccctcgccaa ccgggtactg aataaagaag gcgaagtcga acgcctgctc
    33001 ggcattaata tggatatgac tgaggttaaa cagcttaacg aggcattgtt tcaggaaaaa
    33061 gagcgcctgc acattacgct ggactccatc ggcgaagccg tggtctgtat tgatatggcg
    33121 atgaaaatta cctttatgaa tccagtggcg gagaagatga gcggctggac gcaggaagaa
    33181 gcgttaggtg ttccgctcct ggcggtgttg catattactt ttggcgacaa cggaccatta
    33241 atggagaaca tttacagtgc cgacacctca cgttccgcga tcgaacaaga tgtggtgttg
    33301 cactgtcgga gcggcggcag ttacgacgtg cattacagta ttacgccgtt aagtactctg
    33361 gacggcagca atattggttc ggttctggtg attcaggacg ttaccgaatc acgcaaaatg
    33421 ctgcgccaac ttagctacag cgcctcccat gatgcactga cgcatctcgc caatcgcgcc
    33481 agttttgaga agcaactgcg tatcctgctg caaacggtaa acagtacaca tcagcgacat
    33541 gcactggtgt ttatcgatct tgatcgcttt aaagcggtga atgacagcgc cgggcatgcg
    33601 gcgggcgacg ctttgctgcg cgaactggcg tcgttgatgc tgagtatgct gcgctccagt
    33661 gacgtgctgg cgcggctcgg cggtgatgaa tttggtctgc tgctgccaga ctgcaatgtt
    33721 gaaagcgctc gttttatcgc tacacgtatt atcagtgctg tgaatgacta tcactttata
    33781 tgggaaggcc gggtacatcg ggtaggtgcc agtgccggga ttaccttgat cgatgacaac
    33841 aatcatcagg cggcagaagt gatgtcgcag gctgatatcg cctgttatgc ctcaaaaaat
    33901 ggtggccggg gccgggtgac ggtttacgaa ccgcagcaag ctgccgcaca tagcgaacgg
    33961 gcggcgatgt cgcttgatga acagtggcgg atgattaaag agaatcagtt gatgatgctc
    34021 gcccacggtg tcgcttcgcc acggatcccg gaagcgcgta atttgtggct gatttcactt
    34081 aagctctgga gttgcgaagg agagattatt gatgaacaaa catttcgtcg tagcttcagc
    34141 gatccggcgc ttagccatgc tcttgaccgc cgggtattcc acgaattttt ccagcaggcc
    34201 gcaaaagcgg ttgccagtaa aggtttaagc atcgccctcc ccctttccgt tgccggtttg
    34261 agtagcgcca cgctggtgaa tgaactgctt gagcagttgg aaagtagccc actcccccca
    34321 cgcttattac atctgattat tccggcagaa gcgattttcg atcatgcaga aagcgtgcaa
    34381 aaactgcggc tggcgggatg tcggatagtg ctcagccagg tgggccgcga tctgcaaatt
    34441 ttcaactcgc tgaaagcgaa tatggcagat tacctgctac ttgatggtga gttatgcgcc
    34501 aacgtgcagg gtaatttgat ggatgagatg ctgattacga ttattcaggg gcacgctcag
    34561 cgactcggga tgaaaaccat cgccgggcca gtcgttttac ccttagtgat ggatacgctt
    34621 tctggcatcg gcgtcgatct gatttatggt gatgtgattg ccgatgccca accgctggat
    34681 ttgctggtga atagcagtta tttcgcgatt aactgaggcg tgcttcccca tcgcctgatg
    34741 cgacgctaac gcgtcttatc atgcctacaa atcgctcatt ccccaggccg gataaggcgc
    34801 tcgcaccgca tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca
    34861 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
    34921 cgccggtgtc attcccggaa atcgctgttt aatcagataa tcatccggca ggaaaacatc
    34981 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa
    35041 agtttgcagc gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc
    35101 ctccagcgcc acatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa
    35161 cgcttttaat gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata
    35221 ctccggaaaa tcatccagcc gttcgccata aagctgtgcc actctggcgg tcaattttgc
    35281 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acgccctgct caaaagcatc
    35341 aacactgccg ggtaaacgca atccgggccg cgccgcgcct aacttgccca acgcaccgtt
    35401 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactcagc
    35461 ggcaacgggt tctaaatctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
    35521 aatagcagtc accatgccgc gatattcgcc caccgccaga ctacgggcat aataatcgtc
    35581 agcgaccgtt tccacaccac tcactgcacg ggcggcgaga aatcccaaca tccacgacca
    35641 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag
    35701 cataaacgtt attcagacgc tacgctttgc tttcatattc cggttgtcgc gacggcaaca
    35761 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat tatttcgtgt ttattggttt
    35821 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcacttgct
    35881 aaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaagc
    35941 ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    36001 actgttgcgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    36061 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    36121 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    36181 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    36241 gccagaagcg attactcagg cggtgatcgg tcgccctatc aacttccagg ggcttggcgg
    36301 cgatgaagca aacacccagg cgcaggggat tctggaacgc gcggcgaagc gtgccgggtt
    36361 taaggatgtg gtattccagt acgagccggt agcggcaggg ctggattacg aagcgacttt
    36421 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    36481 gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt gaagccagcc tgctgggtca
    36541 cagtggttgc cgtattggcg gtaacgatct ggatatcgcg ctggcgttta aaaacctgat
    36601 gccgttactg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga ttctgccgtg
    36661 gtggaatgcg gttgccatca acgacgttcc tgcgcagagt gatttctaca gtagtgccaa
    36721 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    36781 acagaaagtc tggcgtcagc gtttaagtta tcgcctggtg cgtagcgcag aagagagcaa
    36841 aattgcgctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcgatgaact
    36901 ggctacgctg attagccagc aagggctgga aagcgccctc agccagccac tgacgcggat
    36961 tctggaacag gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct
    37021 gaccggcgga agcgcccgtt ctccgctgat taaaaaagcg ctggcagaac agttgccggg
    37081 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    37141 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    37201 gagccagttt ttctgccgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    37261 catgaaggcg gaatcgctgc aactctgtcg cgccgacagt caacgccgat tccagccgat
    37321 gtcgtttggc ggccattttc gccattaccg cttgtctttc tgcaggcgtt accgcttcgt
    37381 tcggcctgaa cacaaaacgg cgttcacagc tcgctttcca gtcgatgacc gcctgggtca
    37441 gatgatcacc aaacccttta acttgcttaa ccccacgacg ggtaacatcc gcggctgttt
    37501 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcag
    37561 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcgtgaa
    37621 gtgctgccag agcacgtttt tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    37681 cgagcatcgt ccgtttggcg ataaaacctt ccagcccgcc caactgttgg atctggctga
    37741 ctaaatggtc atagtccatt ttcgcgcgat taaatgcctc acggcgttgc tggatttctg
    37801 ctttataagc tttgcttgtc agactgccaa taatccagat agccgccagc acaggaacca
    37861 aaataatata acgcgattct gcctgaaggc cgcaaagcaa cagcgataac gctgacagtg
    37921 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    37981 tcggttggaa atgatcgggt aatggcaatt gcactgctgg cggtgctact gacgccatca
    38041 ccatcgccca gactttcgcc agcacaaaat tcccgccggt ggtaatgacc tcttcgccga
    38101 gatcaataaa atagataacg ccttgattat ccagcgtaca ccacgggcag tcagtcaaat
    38161 gagcgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagagt
    38221 ccagtgccgc tacccacgct tttgccgtcg gacgcgcggt tgccacgcca ctttccgtga
    38281 atgcctgctg aaacatggct tcaacatcgc ccggtaacat cgacagcgga atcgatcgtg
    38341 gcggcggttt taaaccacgt cgctggttgt ctgacgcgta ggcataacgg aaatgggcaa
    38401 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    38461 gcccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattcgcgg
    38521 tacgttcaaa accgacaaat gatggcaagg tttgtagctc tggcggcgta aaatgcgaca
    38581 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcactatcga
    38641 tcaacaccac tttgctgtcg cgacctacca taaagctgtt ctggtttacg tcccccacga
    38701 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    38761 gaaaatccca cgcacaatga gggtaactct ggcgacgatg tgccgggcta tagatcatat
    38821 gaatcggttc tttaccagaa acttttgaca tcataaaacc gataactttt ccgcctcgcc
    38881 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caactgcgcg tcagctgtcg
    38941 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    39001 cgacgctatc ggcaaactcc tcgatatcat aaaccgcacc ttcaccgcct ttgcccagtt
    39061 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    39121 cagcgccagt gccaacgttt tatcatcatc ggtacgctcg tttaccgccg ggctcgacaa
    39181 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg ccgccagtcc
    39241 attgaaaaac ggggtaaaga aaggcacatg cggagaatta tccagcatat ttaacgccag
    39301 tcgctgaata ccatccgtaa atgctgcaac tttatgcgca cgctcagtgc tggtgaaagt
    39361 ttccagtctg gaaacagcat cttcatcgct aatgaaatgc gtcatattgg catattcacc
    39421 caccatcggc gtgagcggca gttccagacc acgaccaaaa tccaccacta caccgccgtc
    39481 gccaatttgc ataatcaagg tgccgttagc cgatgaaatc aatcccagaa acgtgcaggc
    39541 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatggt
    39601 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    39661 caccataaac gccatcgctt cattgacggc gagcttcgcg ccttctcctc cctgcgaaac
    39721 gctacctgcg ccatcagcaa ggaacatcac cagtaatggc tgctggtcat ttagccaggc
    39781 aacctgcatc tggcaggcat cctgacaagg taaatcagcg ctaatgtgcg acgttccaac
    39841 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    39901 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    39961 aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc
    40021 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    40081 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggtgc accatcggtg
    40141 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cacgatactc acgtttccgc
    40201 tcctctacca tatccagggc tttggtaatg gcggcaccca ttggtgtatc gccctgggca
    40261 aacaggatgg gcgggaaaaa attagcggcg ctggtaaagg gttgttccac atgcaccggg
    40321 ccgaacgtca caataccaag ttccactctt tttaaggcct gcgggtcggc aagcagctca
    40381 tcgcgaaagg taaccaatcc ggcgttaagt tcattaatgg gtctaccgtt catcgaacca
    40441 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    40501 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta ttgaggtccg
    40561 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    40621 cggcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    40681 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    40741 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    40801 catcataacc ctttccttat ttaaggcccc ttcctcggga ggggctttcc cgtttcagcg
    40861 tcccgctgaa atcgtcggct tacctccttt cgccctgaat gcagtctatc gctaacgcgt
    40921 gaacaaaata gttccctctg tgattattgc gtaacgactc gcaaagaata tttgctggca
    40981 ggatcgcaga ctgcaaagcc tgcagattga caatcttatc gtgaaggcat actttcagga
    41041 gtgagggtag agcggggttt cccccgccct ggtagtctta gtaagccggg aagcttatga
    41101 ctaagagcac cacgatgatg atccacttca tcatgaccct ttccttattt aaggcccctt
    41161 cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg
    41221 ccctgaattc agtctatcgc taacgcgtga acaaaatagt ttcctctgtg attattgcgt
    41281 aacatctcgc aaagaatatt tgctggcagg atcgcagact gcaaagcctg cggattgaca
    41341 ttctgaacgt gaaggcatac tttcatggag tgagggtaga gcggggtttc ccccgccctg
    41401 gtagtcttag tatgctgggt agctaatcac taagagcacc acgatgatga gtatcttcat
    41461 catgaccctt tccttattta tggccccttc ctcgggaggg gctttcccgt ttcagcgtcc
    41521 cgctgaaatc gtcggcttac ctcctttcgc catgcaagca gtctatcgct aacgcgtaga
    41581 taaaatagtt tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg ccattcagac
    41641 gattcccgat agtgtttcat aattcctcca tttttctccc ttattggctg gctacactag
    41701 tatcattccg cgaaacgttt caggaagaga aactcttaac gatgaaaggc agttataaat
    41761 cccgttgggt aatcgtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc
    41821 aaggccgcaa tgactcccag agtgcagccc caggggcgac gaaacaagcg cagcaatcgc
    41881 cagcgggtgg tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg
    41941 ccgtagaaca ggcagttccg cgttacctca ccgggcttgg cactattacc gctgctaata
    42001 ctgttacggt gcgtagccgc gtggacggtc aactgatggc gttacatttc caggaaggcc
    42061 agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat
    42121 tagcacaagc ccagggccaa ctggcaaaag ataaagccac gcttgctaac gcccgccgtg
    42181 atctggcgcg ttatcagcaa ctggcaaaaa ccaatctcgt atcccgtcag gagctggatg
    42241 cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg
    42301 ccagcgcgca gctgcaactc gactggagcc gtattaccgc accagtcgat ggtcgcgttg
    42361 gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc gggatcgtgg
    42421 tgatcaccca gacgcatcct atcgatttgc tctttaccct gccggaaagc gatatcgcta
    42481 ccgtcgtgca ggcgcagaaa tccggaaaac cgctggtggt agaagcctgg gatcgcacca
    42541 actcgaagaa attaagtgaa ggcacgctgt taagcctcga taaccaaatc gatgccacaa
    42601 ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg tttcccaatc
    42661 agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaag cgccgtagtg atcccaacag
    42721 ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagc gaaaacaagg
    42781 tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg atccgcgcag
    42841 gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc gaaggggcga
    42901 aagtggaagt ggtggaagcc cagagcgcca ccactccgga agagaaagcc accagccgcg
    42961 aatacgcgaa aaaaggagct cgctcctgat gcaggtgtta cccccgagca gcacaggcgg
    43021 cccgtcgcgc ctgtttatta tgcgtcctgt cgccaccacg ctgctgatgg tggcgatctt
    43081 actcgccggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg aagtggacta
    43141 tccgaccatt caggtggtca cgctctaccc aggtgcgagc ccggatgtca tgacctctgc
    43201 cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc
    43261 gcaaagttcc ggcggtgcgt cagttatcac tttacagttc cagctaacat taccgctcga
    43321 tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt tgccgagcga
    43381 tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct
    43441 cgccgtcacc tcaacagcca tgccgatgac gcaagtggaa gatatggtgg aaacccgcgt
    43501 cgcgcagaaa atctcgcaga tttccggtgt cggcctggtg acgctttccg gcggccagcg
    43561 tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc tgaccagcga
    43621 aaccgtgcga accgccatta ccggcgctaa cgttaactcg gcaaaaggta gcctcgacgg
    43681 cccttcccgt gcggtcacgc tttccgctaa cgaccagatg caatccgccg aagagtatct
    43741 ccagctaatc atcgcctacc agaacggcgc gccgattcgt ctgggcgatg tcgcaactgt
    43801 agagcaaggt gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac aggccattgt
    43861 gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca gcatccggca
    43921 gatgctgcca cagctcaccg agagtttgcc gaaatcggtg aaggtgacgg tactttctga
    43981 ccgtaccacc aatatccgcg catctgtcga tgatactcag tttgaattga tgatggctat
    44041 cgcgctggta gtcatgatta tctacctgtt tttgcgcaat attccggcga ccatcattcc
    44101 cggtgttgcc gtgccgctgt cgttaatcgg cacttttgcg gttatggtgt ttctcgattt
    44161 ttcaatcaat aacctgacgc tgatggcgtt aactatcgcc accgggttcg tggtcgatga
    44221 cgccatcgtg gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa aaccgttggc
    44281 ggcggcgctc aaaggcgcag gtgaaatcgg ctttaccatt atctcgctga ccttctcgct
    44341 gattgcggtg ttgatcccac tgctgtttat gggcgatatc gtcgggcgac tgttccgcga
    44401 atttgctatt accctggcgg tagcgatttt aatctcagcc gtggtgtcgc taaccctgac
    44461 accgatgatg tgcgcacgga tgctcagcca ggagtcgttg cgtaaacaga accgcttctc
    44521 ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg gactggcgaa
    44581 agtgctgaat catccgtggc tgaccttaag cgtggcgctc agcacgctgc tgcttagcgt
    44641 tctattgtgg gtgttcattc cgaaaggttt cttcccggta caggataacg gcattattca
    44701 gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc ggcaacgcca
    44761 ggtcgcggac gtgattttgc aggatccggc agtgcaaagt ctgacctcat ttgttggcgt
    44821 tgatggcact aacccatcgc tgaacagtgc gcgtttgcag atcaacctca aaccgctgga
    44881 tgaacgtgat gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg tggataaagt
    44941 gccgggcgtc gatctcttcc tgcaaccaac gcaggacctg accatcgata ctcaggtcag
    45001 ccgcacccag taccagttta ccttgcaggc cacgtcactg gatgcgctca gtacctgggt
    45061 gcctcagttg atggaaaaac tccagcagct gccacagctt tctgatgtct ccagcgactg
    45121 gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg
    45181 gatcagcatg gcggacgtcg ataacgccct gtacaacgca tttggtcagc ggctgatttc
    45241 cactatttat actcaggcta accagtatcg cgtggtgctg gagcacaaca ccgaaaatac
    45301 cccagggctt gcggcgctgg atactattcg cctgaccagc agcgacggtg gcgtggttcc
    45361 gctaagttcg attgccaaaa ttgagcagcg ttttgcaccg ctctccatca accatctgga
    45421 tcagttcccg gtaacgacca tctcctttaa cgtgccagat aactattcgc tgggcgatgc
    45481 ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca
    45541 gttccagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt
    45601 cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc
    45661 gatcaccatt ctctcgacgc tacccacggc aggtgttggc gccctgctgg cgttaatgat
    45721 tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga tcggtatcgt
    45781 gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg agcaaggcat
    45841 gtcgccgcgc gatgcaatct accaggcttg tctgttacgt tttcgtccga tcctgatgac
    45901 cactctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga
    45961 actgcgtcgt ccgttaggta tcggcatggt cggcggtctg attgtcagcc aggtgctgac
    46021 gctgtttacc acgccggtga tttatttgct gttcgaccgc ctggcattgt ggaccaaaag
    46081 ccgctttgcc cgtcatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg
    46141 cccggtggcg acgattttac tgtcggtcgc cattaccctg tgcggcatac tgggtttccg
    46201 tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc
    46261 gctgcccggt gcgtcgccgg agacaatggc gtcttccgtt gccacgccgc tggagcgctc
    46321 acttgggcgc attgccgggg tcagtgaaat gacctccagc agttcgctcg gcagcacgcg
    46381 tattattttg cagtttgatt ttgaccggga tatcaacggt gcggcgcgtg atgtgcaggc
    46441 ggcgatcaac gccgcacaaa gtctgctgcc cagtgggatg cccagccgcc cgacttatcg
    46501 caaagcgaac ccgtcggatg cgccaattat gatcctgacg ctaacctccg atacttattc
    46561 gcagggtgaa ttgtacgatt tcgcctcgac gcagctggct ccgacgattt cgcaaatcga
    46621 cggcgtcggt gatgtcgatg tcggcggcag ctcgctgccc gccgtgcgcg tcgggctgaa
    46681 cccgcaggcg ctgtttaatc agggcgtgtc gctggacgac gtacgcactg ccatcagcaa
    46741 tgccaacgtg cgtaaaccgc agggggcgct ggaagatggc actcaccgct ggcagatcca
    46801 gaccaacgat gaactgaaaa ccgccgctga atatcagccg ctgattattc actacaacaa
    46861 cggcggcgcg gttcgtctgg gcgatgtggc gacggtgacc gactcggtgc aggatgtgcg
    46921 caacgccggg atgaccaacg ccaaaccggc tattttactg atgatccgca aactgccgga
    46981 agccaatatt atccagacgg tagacagcat ccgggcaaaa ttaccagagt tgcaggagac
    47041 cattccggcg gcgattgatc tgcaaattgc tcaggatcgc tcccccacca ttcgcgcctc
    47101 gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtgtt
    47161 tttattcctg cgctcgggtc gcgccactat tattcccgcc gttgcggtgc cggtttcgct
    47221 gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataacc tgtcgttaat
    47281 ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat
    47341 tgcccgtcat ctggaagcgg ggatgaaacc actacaggcc gcgctacaag gtactcgcga
    47401 agtcggcttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc tgccgctgct
    47461 gttgatgggc ggattgccgg gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc
    47521 cattggtatt tcgttgctgg tttctctgac gctaacgccg atgatgtgtg gctggatgct
    47581 gaaagccagc aagccgcgcg agcaaaagcg actgcgtggt tttggtcgca tgttggtagc
    47641 cctgcaacaa ggctatggca agtcgctgaa atgggtgctc aatcataccc gtctggtggg
    47701 cgtggtgctg cttggcacca ttgcgctgaa tatctggcta tatatctcga tcccgaaaac
    47761 cttcttcccg gagcaggaca ctggcgtgtt gatgggcggg atacaggcag atcagagtat
    47821 ttcgtttcag gcaatgcgcg gcaaattgca ggatttcatg aaaattatcc gtgacgatcc
    47881 ggcagtggat aatgtcaccg gctttaccgg cggttcgcgg gtgaacagcg ggatgatgtt
    47941 tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct
    48001 gcgggtgaag ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat
    48061 tcgcgttggc ggacgtcagt cgaacgccag ctaccagtac acgttgttat ccgacgacct
    48121 ggcggcactg cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact
    48181 ggcggacgtg aactccgatc agcaggacaa cggcgcagag atgaatctgg tttacgatcg
    48241 cgacaccatg gcacggctgg gaatcgacgt acaggccgcc aacagcctgt taaataacgc
    48301 cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat
    48361 ggaagtggat ccgcgctata cccaggacat cagcgcgctg gaaaaaatgt tcgtaatcaa
    48421 taacgaagga aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaatgcccc
    48481 actatcggtg aatcatcagg gattatcggc agcctcgacc atctcgttca acctgccgac
    48541 cggaaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt
    48601 gccttcgacg gtgcgcggca gttttgccgg cacggcgcag gtgttccagg aaacgatgaa
    48661 ctcacaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct
    48721 ttacgagagt tacgtacatc cgctgacgat tctctccacc ctgccctcgg cgggcgttgg
    48781 agcgctgctg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc tgatagggat
    48841 catgctatta atcggcattg tgaagaaaaa cgccattatg atggtcgatt ttgcgcttga
    48901 agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct gtctgctgcg
    48961 ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggcgcgctgc cgctggtgtt
    49021 gtcgggcggt gacggctcgg agctgcggca acccttgggg atcaccattg tcggcggtct
    49081 ggtgatgagt cagctcctga cgctgtatac cacgccggtg gtatatctct ttttcgaccg
    49141 tctgcggctg cgtttttcgc gtaaacctaa acaaacggta accgagtaaa tgacagatct
    49201 tcccgacagc acccgctggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct
    49261 ggacaccacc atcgttaaca ccgcccttcc ctcaatggcg caaagcctgg gggaaagtcc
    49321 gttgcatatg cacatggtta ttgtctctta tgtgctgacc gtggcggtga tgctgcctgc
    49381 cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ctatcgtgct
    49441 gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc
    49501 acgcgcgtta cagggcgttg gcggtgcgat gatggtgccg gtcggaaggc ttacggtgat
    49561 gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt tacccggtca
    49621 ggtcggtccg ctgctcggtc cggcgcttgg cggcctgctg gtggagtacg cgtcgtggca
    49681 ctggatcttt ttgatcaaca ttccggtggg aattattggt gcgatcgcca cattgatgtt
    49741 aatgccgaac tataccatgc agacgcggcg ctttgatctc tccggctttc tgttgctggc
    49801 ggttggcatg gcggtattaa ctctggcgct ggacggcagt aaaggtacag gtttatcgcc
    49861 gctggcgatt gctggcctgg tcgcagttgg cgtggtggca ctggtgcttt atctgctgca
    49921 cgccagaaat aacaaccgcg ctcttttcag tctgaaactg ttccgcactc gtaccttttc
    49981 gctgggcctg gcggggagct ttgccggacg tatcggtagc ggcatgttgc cctttatgac
    50041 gccggttttc ctgcaaattg gccttggctt ctcaccgttt catgccggac tgatgatgat
    50101 cccgatggtg cttggcagca tgggaatgaa gcgaattgtg gtacaggtgg tgaatcgctt
    50161 tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctggtca ccctgttgtt
    50221 tatgactacc gctctgttgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg
    50281 gatggtcaac tcgacccgtt tctcctccat gaacaccctg acgctgaaag atctcccgga
    50341 caatctggcg agcagcggca acagtctgct gtcgatgatt atgcaattgt cgatgagtat
    50401 cggcgtcacc atcgccgggc tgttgctggg actttttggt tcgcagcatg tcagcgtcga
    50461 cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt ttatcatcgc
    50521 ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc
    50581 gcggcgaaaa aggagcgcgc aatgaagttc tggcgacccg gtattaccgg caaactgttt
    50641 ctggcgattt tcgccacctg cattgtcttg ctgatcagta tgcactgggc ggtgcgtatc
    50701 agttttgagc gtggctttat tgattacatc aagcatggta atgaacagcg attacaactg
    50761 ttaagtgatg cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac
    50821 aatgatcgtt ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa
    50881 cccggcccgg gtatgccatc acacggctgg cgtactcagt tctgggtggt tgatcaaaac
    50941 aacaaggtgc tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt
    51001 ctggtcaacg gtgcggaagt gggcgcggtg attgcctccc ccgttgagcg gctgacccgc
    51061 aatactgata tcaatttcga taaacaacag cggcaaacca gctggctgat tgtcgccctg
    51121 gcaacgttac tcgcggcgct cgccaccttt ctgctggcgc gtggtctttt ggccccggtc
    51181 aaacggctgg tggacggcac gcacaaactg gcggcgggcg atttcacaac ccgcgtgacg
    51241 cccaccagtg aagatgaact gggcaaactg gcgcaagact tcaaccagct cgccagcact
    51301 ctagagaaaa accagcaaat gcggcgcgat tttatggccg atatttctca cgaactgcgt
    51361 acgccattag cggtgctgcg cggtgaactg gaagccattc aggatggcgt gcgcaaattc
    51421 acgccggaga cggtggcgtc tttacaggcg gaggtcggta cactgaccaa actggttgac
    51481 gatctccatc agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta
    51541 gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt cgccagccgt
    51601 ggcctgaaac tgcaattttc cctgccagac agtattaccg tatttggcga tcgcgaccgt
    51661 ttaatgcagt tattcaataa cttacttgaa aacagcctgc gctacactga cagcggcggc
    51721 agcctgaaaa tctctgccga gcaacacgac aaaacggtgc gcctgacctt tgccgacagt
    51781 gcgccaggtg tcagtgacga tcagctacaa aaattgtttg aacgttttta tcgcaccgaa
    51841 ggttcccgca accgtgccag cggcggttcc gggctggggc tggcgatttg cctgaacatt
    51901 gttgaagcac ataatggccg cattattgct gcccattcgc cttttggcgg ggtaagcatt
    51961 acagtagagt taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg
    52021 acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca
    52081 ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac
    52141 tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt agatctgatg ctccctggca
    52201 ccgatggcct gacgctgtgc cgggaaattc gtcgtttttc tgacattccg atcgtgatgg
    52261 tgacggcaaa aatcgaagag atcgatcgcc tgctggggct ggagattggc gcagatgatt
    52321 atatctgtaa gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc
    52381 gttgcaaacc gcagcgcgag ttgcagcaac aggatgctga aagcccgttg attatcgacg
    52441 aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacgcct gcggaatttc
    52501 gtctgctgaa aacgctctct cacgaaccag gaaaagtgtt ctcccgcgag caattgctca
    52561 atcatcttta tgacgactac cgcgtagtaa ccgaccgcac catcgacagc cacattaaaa
    52621 acctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttatg
    52681 gcgtcggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacattatt
    52741 ttgttagccg gagatggcgt tcagaccaga tccggcaaca ttatcccacg catggtcagc
    52801 aaactgacat tatctctccg ccctggcagg catcagaaaa tcgctcatac tttaatcggt
    52861 aaacagcacc tttagatgct gttttcgata cacaatttca atcaaggagt cattatggct
    52921 ggttggtttg aactcagtaa gagcagtgat aatcagttcc ggtttgtgct aaaagcgggc
    52981 aatggtgaga ctatcctcac cagcgagctt tatacctcaa aagcctctgc ggaaaagggc
    53041 atcgcgtcgg tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa aacggcaagt
    53101 aacggcaaat tctatttcaa tctgaaagcc gctaatcatc aaattatcgg ctccagccag
    53161 atgtacgcca ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca
    53221 agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt tggcgtccgg
    53281 cgctttacca gatcccccca cagcgctaca atgcccgccc ttaaagtggg ggcactcccc
    53341 taaccgcttc atcaggtgaa gcggatctga cctgtcatca gaacgagaga attatgttta
    53401 aaccggaact cctttccccg gcgggaacgc tgaaaaatat gcgttacgct ttcgcttatg
    53461 gcgcagatgc tgtttatgcg ggccagccgc gttactccct gcgtgtgcgc aacaacgaat
    53521 tcaaccacga aaatcttcag ctcggcatca atgaagccca cgcgctgggg aaaaagtttt
    53581 atgtcgtggt caacattgca ccgcacaacg ccaagctgaa aacctttatc cgtgacctga
    53641 aaccggtggt ggaaatgggg ccggatgcgc tgattatgtc cgatccaggg ctgattatgc
    53701 tggtgcgtga gcacttccct gaaatgccga tccacctctc ggtgcaggct aacgccgtaa
    53761 actgggcgac ggtgaaattc tggcagcaaa tgggcctgac ccgcgtgatc ctctctcgcg
    53821 agctgtcgct ggaagagatt gaagagatcc gcaatcaggt gccggatatg gagatcgaga
    53881 tcttcgttca cggcgcactg tgcatggcct actccggtcg ctgcctgctc tctggctata
    53941 tcaacaagcg cgatccgaac cagggtacct gcaccaacgc ctgccgctgg gagtacaacg
    54001 tccaggaagg gaaagaagat gacgttggca acatcgtaca caagtacgag ccgattccgg
    54061 tgcaaaatgt tgagccgacg ctgggtatcg gcgcgccaac cgacaaagtg tttatgatcg
    54121 aagaagccca gagtccgggc gagtatatga ccgcgtttga agatgagcac ggcacttaca
    54181 tcatgaactc gaaagatctg cgcgccatcg cccatgtaga acgcctgacc aaaatgggcg
    54241 tgcattcgct gaaaatcgaa ggccgtacta aatctttcta ctattgcgca cgcaccgcac
    54301 aggtttaccg taaagctatc gatgacgccg ctgcgggcaa accgttcgat accagcctgc
    54361 tggaaactct ggaaggtctg gcgcatcgtg gctataccga aggtttcctg cgtcgtcata
    54421 ctcacgacga ttatcagaac tacgaatacg gttattcagt ttctgaccgc cagcagtttg
    54481 ttggtgagtt taccggtgag cgcaaggggg acctcgcggc ggtagcggtg aaaaataaat
    54541 tctccgttgg cgacagcctt gagctgatga cgccgcaagg caacattaac tttacccttg
    54601 agcacatgga aaacgccaaa ggtgaagcga tgccggtcgc accaggcgat ggttatactg
    54661 tgtggctccc ggtcccgcag gatcttgagc tcaattacgc tctgctgatg cgtaatttct
    54721 ccggggaaac cacgcgtaac ccacacggta agtgattaat ttcgattatt tttcccggat
    54781 ggaaaattct tagaaaccga tcacatacag ctgcatttat taaggttatc atccgtttcg
    54841 ctgaaaaaca taacccataa aatgctagct gtaccaggaa ccacctcctt agcctgtgta
    54901 atctccctta cacgggctta ttttttatgc ataagcccta tccctggtca ccgtcttcca
    54961 ttgaccacat cgatagaatc tcccttcata gcacgatgcc tttcacgtaa cggcatcgtg
    55021 ctcgcacagg ttccggctaa gcacaaccag aacgcacata tttgacgctt accaaaaaat
    55081 attctcactc tccacatttg aatgtcagac gagcgacacc atgtaatcct acaccttctg
    55141 tcttcagctc aactatttgc atttttttgc cctgagtaac acagaaatgg gctgcatcat
    55201 tttttactat attttctgca ccagatattc tacccctggc taaagaagct tcggcttcgg
    55261 tgtagtattg gttatcgagt ttacgctgaa tattactttt atatgcaagg ccaaatttac
    55321 cgatacttgt ctcatcatta tgcacagcac aaccagacat aagaaaaaca ctaattaatg
    55381 atatagcagc tattttattc acctcacctt cccccattaa ataccaacga cactctctag
    55441 tgtttaagta taataatggc cgatcattat aattgaatag gattataata aatgttttgt
    55501 acaacatttc ctacataagt aggaattaca gactcagagg cccttccggg taaccctatg
    55561 ggagctttaa tatatttgga atagtatatt gaagatgcca ctgtttagtt gaatattaga
    55621 tatttgctct tttttcaaat ttaccggtgg ctaccgttaa cattcactgc ccccattgca
    55681 agctcctggt ggtaacaact gaattaccca tgcttgaact gactttttat cctccgactt
    55741 tcatcctgtt ctgactccac cttttgtttt ctgctctaca ctatctacag accaatcata
    55801 aaggcacata cgatcatggc agaatttccc gccagcttac tgattcttaa tggcaaaagt
    55861 actgacaatc tacccttgcg cgaagcaatt atgctgttgc gtgaggaagg aatgacgatc
    55921 catgtgcggg tcacctggga gaaaggtgat gccgcacgat ttgtagagga ggcccggaag
    55981 ttgggcgtcg caacggtgat tgctggaggt ggcgatggca ccattaatga agtttctacg
    56041 gcgttgattc agtgtgaggg ggatgacata cccgcgctgg gaattttacc attaggaacc
    56101 gccaatgatt ttgccaccag cgtagggatt cctgaggcac tggataaggc gctgaaactg
    56161 gcaattgccg gtgacgccat tgcgatagat atggcgcagg tcaacaaaca aacctgtttt
    56221 attaatatgg cgacaggcgg atttgggacg cgtattacca cagaaacgcc ggaaaaatta
    56281 aaagccgcgc tgggtagcgt ctcttacatc attcatggct taatgcgtat ggatactctg
    56341 caaccggacc gttgtgaaat ccgcggtgaa aactttcact ggcaaggtga cgccctggtc
    56401 attggtattg gtaacgggcg tcaggccggt ggcggtcagc aattgtgtcc gaacgcgtta
    56461 attaacgatg gcttgctgca actgcgcatt tttaccggcg atgaaatact tccggctctc
    56521 gtatcaacct taaaatctga cgaagataac ccgaatatta tcgaaggcgc ttcgtcgtgg
    56581 tttgatattc aggcaccaca cgacatcacc tttaatcttg atggcgaacc gttgagtggg
    56641 caaaattttc atattgaaat acttccggca gcgttgcgtt gccgtctgcc accagattgc
    56701 ccgcttttac gttaaataaa agcaagcgca gaagcaggat tagcagaccg gcatcatccc
    56761 tgggatcttc tgcgctcacc cttttttgtt acgcagctac ggctaacgct gccagtaatt
    56821 cattcatcga taaatatctt gcactcgccg cattaaagca ggtttgttgg gcatcttctg
    56881 ataccacaat ggtgtttatt cctgccgcct gtgctgcctg aaaaccactc acgctgtcct
    56941 ctataaccaa acaatctgca gcagacagtt gcaattctcg cagtgcagaa agataaactg
    57001 ccggatgggg tttcccatgc ttttcgtcat cagcactaca aatcaccgaa aaataatgct
    57061 gaatattgag ttttgtcagt acagcatcga tcacctggta agatgaagag gttgccagcg
    57121 ccagttgata gccactccgg cgcaaaaaaa ataacacgtc ctgaacgcct ggcatcgctt
    57181 cgcccgtccc ggcaatcaac gtagtgatca ggcggcgaat ctcactttcc aacagtgcgg
    57241 gttcaatgtg taaatgacaa tattcacacc aggtttgggc aatctcatcc agccttttac
    57301 ctttagtcag tcttatacac tcttcgtcac tgacacatgc gccccacctt gccagtgctc
    57361 ccttttgtgc ctcacgccat aacccttccg aatcaatgat gacgccgtcc atatcaaaaa
    57421 tgactgcttt acaattaaac attgttcctc gcaataaagc ctggcaccac accaggcttg
    57481 tcgtcatcag gaaatggtgg cggtcttccc ttcacacact tgctgatttg agaatcccgg
    57541 ttgttcgacg cgaataaacg ggcaaataat aaacgctatc acatacaggg tggtgtaagc
    57601 gataaccaca ccaatagtac tgaaataagg cagaagtacc acggcgattg ccggagcaag
    57661 gaagttggac aacccggctg aaaggttata aaccgaaata gctgctcctt tatgtttcgg
    57721 ttccagtgcc gggaaaacgg ctgccatcgg tacgaatgca gcaacaaaga tccccagtgc
    57781 caccgcaggt atcagcgcca tagcaaagtt atgaccgaaa tgttgcggca tgtagtagaa
    57841 cgccaggctt gaaagcgcca ttcccacgca accaaaccag cgaacaactt tcatccagcc
    57901 cattttctct gccagcacgc cccagaatac gttagagaag atggtggtaa agaagaagac
    57961 tgcccagacc tgcaaccact cagaggtggt aaagcccagc tcatcaacaa acatcatcgg
    58021 cataattacg gcgaatccga ataaagataa ggtgttgata atgcgaacaa tactggaaag
    58081 caaaatattg cggttggtat aaagcaaagt caccgcacgg cccagttcgc taaacttctc
    58141 ttttgtggtt aggttttgca tatggcgtgg tgtttctact ttgcgcatag aaattaacgc
    58201 aacagcgcca ccaaccccac agaaggccag cgccagccat aatgttccca tttcgccaat
    58261 aagcggaatg gtaaaactgg ggatgtagct gccgaatacg ccgataccaa tagagtaaac
    58321 tgcccagaac cagcccagag cagcactggt gctgtcactg cgtacgttat ggacaatcgc
    58381 cacaataaag gcgtagagga acagcggata ggctaggcca cgaataccat aaaacagcag
    58441 gattaaccca tagttcgcct gccccagacc aaagaccaga aacagcacgt gaaatacgca
    58501 ccacagcaca aagccgataa acatggtttt ttgtggcgta ataatttcgg caactacgcc
    58561 ggaaacccag gcggaaagcg ctgccgccag gccatagagc gtaaaggcaa aggaggcttc
    58621 tgcaggcgtg aaacccagtg ctttaatgta atgcgacagg aatgccagtt caaaaccgtc
    58681 tccggtcata aagatggcaa tggcaatata tccccacacc agatttaagg gtaatcccag
    58741 ccattgttta tttgttaacg acatagtgac ctcgtttgta ggatacaaaa cgccgctccg
    58801 gtgcaaacac cggagggcat ttgtgacaga tgttattgat tgcgatagtt caggtcgttg
    58861 tgatacagcg cagtaaactg ctgatatcgt tgcatcagcg cattatgacg ttcaggatcg
    58921 ctgcgccagg ttttgaagat ttcaggtttt tcacacactg ccgccagtgg ttttcccgca
    58981 gcaagacagg ccagacgcgc ggctcccagc gccccccccg tttcaccgcc tttatgcgtc
    59041 accactggca tatcgagaat atcggccagt aactgcgccc agaatggact tctcgccccc
    59101 ccacctacca gcgagcattg ctcaattgcc gtaccgcttt cttgcaggac tcgcagccca
    59161 tcggcaatac caaagctgac gccttccagc actgcatatc ccattagcgc acgctgactg
    59221 gagtgtgtca gcgcccagaa caatccccgg gcatgaggat cgttatgtgg cgtacgttca
    59281 ccagaaagat acgggaggaa catcggtgca ctggctttgt ctgcatcaga aagttgggca
    59341 atctcttcca gtaactctgt ttccgttacc cccaccagac ggcagaacca ttgcagacaa
    59401 ctggcggcac tgagcattac actcatctgg tgccataaat tcggtagtac gtgacagaaa
    59461 gcatggactg ctgattgcgg cgcaggacga taggcatctg tcactacaaa caacaccccg
    59521 gaagttccca aagaaataaa cgcatctccg ggagaaacgg cccccacacc aattgcgctc
    59581 acggcgttat cgccaccgcc accagcaact acaaccgaag atgtcagccc ccaacggctg
    59641 gcaacttcag gggaaagagt ggcagaaacg tcacaacctt ccaccagttc cggcatattc
    59701 gcccgtgtca ggccacattt ttccagcaac gaatctgacc agtcgcgttt ttccacgtcg
    59761 agccacaacg tgccagcaga atccgacata tcggaaatct tttggccggt cattttgaaa
    59821 cgcaaatagt ctttcggcag taaaacggtg gaaatgcgcg caaagtgctg cggctcatgg
    59881 cgccgtaccc atagcaattt gggggcggta aatccaggca tcgccaggtt tcccgctacc
    59941 tgatgcaact ctggtgcgat ctcttccagc tccgcacact cctgcgcact gcgggtgtcg
    60001 ttccataaaa tcgccggacg aatcgcctct cctttttcat ccaacaggac ggcaccatgc
    60061 atttgaccag aaaggccaat ggctttgatc gcctgccagt ggtgaccgca tttttcccgc
    60121 agggtcgaca ttaaatattc cgtcgcttcc caccaggctt gcggcgattg ttctgaccag
    60181 tgtggatggg gccgctgaat ggtcagcggc gcactatggg tggcaacaat ttcattgttt
    60241 tcatcaatga ccagcgcctt aacttccgat gtgcccagat cgatacccag atacataacc
    60301 gcctcctggt tagttaatta acgcgtagac gtcagcgatt ttttcgcgca acaacgcggc
    60361 aaaatcttca cgttcggtta aatcgccaaa cagcgcttta tcactggcat aaacagcgac
    60421 gggatcggca gactggaaca ttgcatggac agctggtgca tcaaggatgc catcctgata
    60481 ttcatagggc agtttgccgt gatgccattg ctccatgaat acgtaaaaca gtgcaggtaa
    60541 catggcggtg gcattcgggc gaacgcctcg ctggtagcac tctcgcagtg tgggggcaat
    60601 catcgccgga attttcgaga aaccatccgc agcgacgcgt tggttggtgt cctgaatatg
    60661 tggattggta aaacgcttga gtacaacatc acggtaggtt ggcaaatcga taccgttatc
    60721 gcccaagcaa ggaatgacat cttccgtcac gtagcggtcg gcaatctgat agataaaatc
    60781 ggtcattgtg ctttcgtgga tatatttttg accgattaac gtacctgccc aggcgatgca
    60841 actgtgtgaa gagttaagaa tgcgaatctt cgcctcttca taggggatta ccgacgccac
    60901 cagttcgaca ccgaccttct ccagtgccgg acggacatca cggaaattat cttccacgac
    60961 ccactggata aaggtttcgc ccattaccgg cgctttatcg gcaatacccg tttgagcctt
    61021 gatccgtgcc ggaagttctg ctgccggacg aggcgtaatg cggtcaacca tggtattcgg
    61081 gcaagtggta tttgtactca gccagtcgat gacatcctgt ttgccagtta gctggagaaa
    61141 ctcaaccagg ccatcatgga aacgttcacc attatggcgc acgttatcgc aattcagcag
    61201 ggttagtggt ccggcgttat ttgccatacg tgcttcgagg atacgggtaa taacaccgta
    61261 aattgttttg catccccctt taagatctgc cgctaaatca ggattgttaa cttccagttt
    61321 gtgactggta ttcaggtagt acccgccttc ggtgacggtg aaagcaatca cttttgtctt
    61381 cggatctgcc ccttcagcaa tcagcggttg taaatctgcc tgccacggta tcaacttctg
    61441 aattgaggtg atctcttcat attcgcttac cccttccggg ctgacggttt ccagcacata
    61501 gcgacctttc tgtgcactga gtgcctgtac gacatgttca gcatcattac gaatattgcc
    61561 cgcagcaatg ctccagcgtt tatcgcccat cacctgcaaa cggtgtagat accacgcctg
    61621 atgtgcgcga tgaaaagaac ctaacccgat gtgcagccat gtaaattgtt cgttcattgt
    61681 ttcgttctcc attgacgtta tggctgaact gtaatcgaac atttgagtta attaagagcg
    61741 ttagatcaca aaagaacaat tgaccaaaaa aattacaaat gaccaaatca aagcaataaa
    61801 cttgcctcgc ggtaaagcta tggccaaaat gacgctaatc agttgcggga ttttcacgat
    61861 tccgcacgcc acttttgagg tagggaaacg tgaacaaaga agatgacatc aggctcgatc
    61921 aaaaagtacg ggcggcatgg atgtactaca ttgccggact taatcagagt gaaatcgcaa
    61981 gtcagttagg tacttcacgg ccagtggttc aacgcctgat agcagcagcg aaagacgaag
    62041 ggattgtgtc gattggattg catcatccgg ttgccaactg tctcgattat gcccaactgt
    62101 taaaggaaaa gtaccaatta accgactgca atattatgcc ggtttacagt attgaaagta
    62161 cgctcgacag cgtgacattt ggctgttatc aactgatggc gcgttactta cagggtgaca
    62221 aaccgaccat cgttggtatt ggttctggct taacgttgaa gaaaaccatt aaacgtatcg
    62281 attttgatag tcagaatacc cgctgtgtgg cgctgattag cgccatgaat gaacacgggc
    62341 aatgtaacta ttacgacgac gtaccgctgc ttttggcgag aaaaattcag ggaaaatatt
    62401 accagtggcc agctccacgc tatgctcagt cccgcgaaga gtaccaaatg tggtcaacca
    62461 atcgattgtt ccgcaacgtc tctaccgtag cgcgtgactc tgatgttatt ttcgtgggta
    62521 ttggtgcttt aggtcgtcaa agccctattt tcaaagatgg ctttattgat gagcagcaac
    62581 tggaagagtt aacagcacgc ggaggcgttg gtgaaatcct cggacgcttt attgatgctg
    62641 aaggcaatgt tgtggaaagc cagatcaacc agatgatcac cagccatgat atccgcgaaa
    62701 gcaactgccc acgtatcgca gctgcctgtg gtgaggataa acgtccggcg atcctcgcag
    62761 cattaaaagg tgggtggatc aacggtctgg tcaccgatga acacaccgca cgctggttgc
    62821 tgacacgtta atctcagctg acaacattca ggctcggaat gagttgtggc ggtatctcaa
    62881 tgcgacgcag ggtggtatcg ccagaatcca caatttcaaa cactttttcg agcatggcgg
    62941 gtacatcctg acgaaccata tcgatatcgt gcccgagcag agaaacaaaa ggatcccagt
    63001 caaaacaccc cattggcggt tgattggctc ccgtctgccc ggtttgcgcc agccagcgaa
    63061 ccaccccttc aagagagatt gtcgagttga cgaataaccc gtgaagcgat actgcagagc
    63121 tttggaagac ctctttcagt aatccctcta cgcgagcaga agagtagccg gtaaccagtt
    63181 tatgttcatc cggtacggtt atccccaaag cctgatgggc atcgacaaaa ccacataaac
    63241 gttcagcagt attgttatcg cttatacgtc caccgacaaa agtcaaaggt gccagcttgc
    63301 catagcgttt ctggctgttg gctaaaattc tttccgtcaa taatttcgcc cccatatagt
    63361 tatcagaaat gatcgacggc gccagtgttc ccggcagatc cagattcagt gtcggtacgc
    63421 ccgcttgctg gcacatttgg gctattttat ccggttgagt cgctccggta gcaatgaccc
    63481 aatccacctg ccaggagagc atggcgcgag cagcctcaat ttccagttct ggctgtcgcc
    63541 gcgtacaggt gatgattggc agtaatcctc tggcacgcgc catctcttca aatttttcgg
    63601 caatcgatcc aaagtatcgg ttgtcatatt tcggcacaat catgccaata acgttggatt
    63661 ttttactgcg cagtaaactt gcctgccgat taatggcata tccctgctct tccgcaatac
    63721 gggtgacctt ctcagccagc tttgcgctga tgcgtcgctt tttccagttg ccattcaaaa
    63781 tagcgcttac cgcactggca gaaacgccgg ttaattctgc caggtcgtaa atggtcgttt
    63841 tcttcgtttt ctcaacgcgt gtcacaaatt tgcctcttca ttttgaatgt ctcttgctga
    63901 gaatttaagc aagtgctaga tctgcttcat cgattgagca gtttgcttca cacggcagct
    63961 aaattcccgt tcagtgcgtg caagcaaaca gagactataa attcgccctg gtaaaaggat
    64021 tatatgatga atcactctgt gccctctatg aatactcccc ttaatggcaa agttgcagct
    64081 atcactggcg ctgcgtcagg tattggcctg caatgtgcaa aaacgctgct cgatgcagga
    64141 gcaaaggtag tattgattga ccgggaaggc gacaaactgc acaagattgt cgctgagtta
    64201 ggcgaaaacg cgtacgcgct gcaactcgat ctcttcaata atcagcaagt cgataacatg
    64261 ctggcggaca ttatcgaact ggcgggtggg ctggatattt ttcatgccaa tgcaggcgct
    64321 tatattggcg gcccagtggc tgaaggtgat ccagatgtct gggatcgtgt gttaaatctg
    64381 aatataaatg cggcgtttcg ctgtgtccgt gcagtcctgc cgcatatgat tgcgcagagg
    64441 tcgggcgata taatttttac cagttccatc gcgggcgtcg ttccggttat ctgggaaccg
    64501 atctacaccg cgtccaaatt tgccgttcag gcattcgtac acactacccg ccgccaggtt
    64561 tctcaatatg gcgtgcgtgt gggtgcggtg ctgccaggac cagtagtcac tgccctgctt
    64621 gatgactggc caaaagccaa aatggaagaa gccctggcaa atggtagcct gatgcaaccg
    64681 attgaagtgg cggaatcagt attgtttatg gtgacccgct cgaaaaatgt caccgtgcga
    64741 gatttagtga tcctgcctgg cagtgtcgat ctgtaagggc gcaatcatga caataaccaa
    64801 aaccgttatt ggtgttgatg tgggatcagg cagtgtccgc gccgggattt ttgatctcaa
    64861 cggatctctg ctatcccatg ccacagaaaa aatcacgact acgcggcgca gcggaagccg
    64921 cgtggaacag tccagccagg agatctggca ggcggtctgt tcatgtattc gtaatgcgct
    64981 cactctggca gacgtttgtg cacaaagtgt ggcaggcatc ggttttgatg ccacctgttc
    65041 tctggtggta ctggataaaa acggtgatcc attgcctgtc agcccggaag gagatgcaaa
    65101 gcaaaatatc attgtgtgga tggatcaccg cgccaccgaa caagcagagc gaatcaatgc
    65161 cactcaccat ccggtgctga actacgtcgg tggtaaaatt tcgcctgaaa tggaaacacc
    65221 gaaaattctc tggctgaaag aaaatatgcc agagatctac gaacgtgccg gacaattttt
    65281 cgatctggcc gattttctga cctggcgggc taccggtgat ttagcgcgtt cagtatgcac
    65341 tgttacctgt aaatggacgt ggctggcaca tgaaaatcgc tgggatccag attatttccg
    65401 caccattggc cttgcagagt tagcggatga agattttatt cgcattggtc atcatattgt
    65461 ttctcccgga acaccttgtg gaaatggttt aacagcacaa gccgcggcag agatgggatt
    65521 actccccggc acacctgtcg ctgtagggtt aattgatgct cacgctggtg gcatcggtac
    65581 ggttggcgta gaaggtggag cgctgaacaa tctcgcgtat gttttcggca cttcttcatg
    65641 caccatggca tctaccactt ctccctcgtt tgtaccgggt gtctgggggc cgtattacag
    65701 tgcgatggtt ccaggtctgt ggttagttga aggcgggcaa agtgctgcgg gagcagctat
    65761 tgaccagcta cttgatttcc atccggctgt tgaagaagct cgcgaaatgg cacaacgtgt
    65821 gaatcagccc ctccccgtct ggcttgctga tcgaatcctc gaaaaaacgg cgcaaccatc
    65881 agatgctgtc gccctggcga aagggctaca cgtggtgccg gaatttctcg gaaatcgcgc
    65941 ccccttcgca gatcctcatg ccagagcggt aatttgtggc ctgggtatgg agcgagatct
    66001 ggataattta ctcgccttgt atatcgctgg attatgcgga attggttatg gtctgcgcca
    66061 aattctcgac gctcaaacag cgcagggagt agtgagtaaa aatatcgtta ttagcggcgg
    66121 tgccgggcag catccactgg tacgacaaat tctggcagat acctgcggta ttccggtcat
    66181 taccacgcaa tgctgcgaac cggttttatt aggctcggcc attcttggtg ctgtcgccgg
    66241 aaatattgca ccttctgttg gcgaagcgat gcaacaattc acccatgtgg ataaatatta
    66301 ttatccgcaa gaacgctatc aatctcttca tcatcgtcga tatgaggcct ataagcagtt
    66361 gcagcatact gcaaaattac tcagagacta attaaccagc cgcctgacgc tgttttcagg
    66421 caatcactaa taacgactca ctccggtaat atcccggagt gcattcatct gcaccctaaa
    66481 aacgaggttt atatgtccag aaataataaa cagtggttgg gtttgccact gcatctgata
    66541 tggggatata tcgccatcgc agtatttatg actggtgatg gtttcgaact cgcattctta
    66601 tcgcactata ttaaatcgtt aggcttcaca cctgcggaag cctcttttgc ctttacgctc
    66661 tacggcctgg cggctgccct ttccgcctgg gtttctgggg tagtagcgga aatcatcacg
    66721 ccgcaaaaaa ccatgctgat tggttttgtc ctatggtgcg tattccatgt tctgtttctg
    66781 gtctttggat taggacaggc aaactatggg ttaatcctgc tgttttacgg gattcgtggt
    66841 ttagcgtatc cgctatttct ctactcattt atcgtcgtta ttattcataa cgtgcgtagc
    66901 gaaaattcca gttctgctct gggttggtac tgggcggtat attcagtagg gatcggtgtt
    66961 gctggcagtt atattcccag ttttacgata ccgattatgg gtgaaatggg aaccttatgg
    67021 ctggcactgg cgttctgctt tgctggcggt gtcattgcca tgatctcctt gcgtcatgtt
    67081 aaaacgcctg gacatatgca taatttaact ccccgtgaga agtttgcaga attaagtcgg
    67141 gcagtaactt tactttatac caaccgcaat atttttctct ccagtattgt gcgcattata
    67201 aataccttat cgttattcgg ttttgcggtc attatgccaa tgatgtttgt ggatgaactg
    67261 ggattcacca cctctgaatg gttgcaggtc tgggcggcat ttttctttac cactattttc
    67321 tctaatattt tttgggggat tgtggcagaa aaaatgggct ggatgcgtgt tattcgctgg
    67381 tttggttgcc tcgggatggc agcatcaagt ttagcgtttt actacatgcc gcaatacttt
    67441 ggtcacaact actggatggc aatgattccg gcgattgctc tgggaacttt tgttgctgca
    67501 tttgtgccga tggccgctgt cttcccggca ctggaaccaa aacacaaagg tgctgcaatc
    67561 tcggtttaca acctctctgc gggtatgtct aacttcctgg ctccggcaat tgccgtggtg
    67621 ttattaccgt ggtttagcac tatcggtgtg gtcattgcct atacagcatt gtatctattg
    67681 gcctttgtcc tttgcgcatt cattcgcgtt gagcagccag gattcagttc tgcgccagtg
    67741 actgagaagg cattgaatat ctcctgaaaa acgaaacgca tcaggcactc atcctctccc
    67801 tcatgggaga ggatgatttc acatcaggca atagtgactt tgttatcgag ataaacgtcc
    67861 tgcacggcgt taatcagttt cacgccgtca gccatcgatt tcttaaatgc tttccgcccc
    67921 agaatcagcc ccattccgcc tgcgcgtttg ttgataaccg cagtacgcac tgcatcgctg
    67981 aggtcagttt caccgcccgc agcaccgccg gagtttatca acccagcccg acccatatag
    68041 cagttagcta actgataacg caccagatca atcgggtttt cgctggtcaa tttgctgtaa
    68101 acacgatcgt cggtgtaacc gtaattaatt gctttatagc cgccgttatt ttccgccatt
    68161 ttttgtttga cgatatctgc accgatggtt gccgccagat ggtttgcctg accggtcagg
    68221 tcggcggaaa catggtaatc aacgccatct ttcttaaagg cggagttacg caaataggcc
    68281 cacagcactg tcaccatacc cagctcgtgc gcacgttcaa aagccgcaga aatttcttca
    68341 atctggcgac gtgactcttc cgagccaaaa tagatagtcg caccaaccgc aaccgcgccc
    68401 atgttgaacg cctgctccac gctggcatac agcgtttgat cgtaggtatt cgggtaactt
    68461 agcgtctcgt tgtgattaag tttgacgagg aatggaatgc gatgcgcata acgccgcgat
    68521 accgacgcca gcacgccgta agttgacgcc acacagttac agcccgcttc gatcgccagt
    68581 tcaacaatgt ttttcgggtc aaagtagagc gggttagcag caaatgaagc tccggcagag
    68641 tgctcaacgc cctggtcaac cggcagaata gaaagatatc ctgtgccagc cagacgcccg
    68701 gtgttgtaca acgtctgcat attacgtaac accgctggcg ggcgattatt gtcaatcatt
    68761 acgcggtcta cgtagtcatg tccggggaga taaagctggt cagaaggaat tgtcatacaa
    68821 cggtgctgta aaaggttgtc ggcgtctttg ccaagcaact gcgcaatatc tgtcattact
    68881 atgctcccgt aaattccgat tggatatcgg ctatggattg tcctggcccg ccttttgcgg
    68941 gcaatcataa tcctggtcgt tacgggcgag tttttccatc ttttggatgt tttttcagcg
    69001 ttttctgttg gctcgattca tcagaaaaaa tgttagcgcg gtcaagtttt caccgcaaag
    69061 gtatttaaaa ggtattatta agtggtattg tcatcgcgta ccttacatta cctgtcatga
    69121 aggaattaaa agatgaaaac aacagcaaag ctgtcgttca tgatgtttgt tgaatggttt
    69181 atctggggcg cgtggtttgt gccattgtgg ttgtggttaa gtaaaagcgg ttttagtgcc
    69241 ggagaaattg gctggtcgta tgcctgtacc gccattgcgg cgatcctgtc gccaattctg
    69301 gttggctcca tcactgaccg ctttttctcg gcgcaaaaag tgctggcggt attgatgttc
    69361 gcaggcgcgc tgctgatgta tttcgctgcg caacagacca cttttgccgg gttctttccg
    69421 ttactgctgg cttactctct aacctatatg ccgaccattg cgctgactaa cagcatcgct
    69481 tttgccaacg tgccggatgt ggagcgtgat ttcccgcgca ttcgtgtgat gggcactatc
    69541 ggctggattg cctccggtct ggcatgtggt ttcttgccgc aaatactggg gtatgccgat
    69601 atctcaccga ctaacatccc gctgctgatt accgccggaa gttctgctct gctcggtgtg
    69661 tttgcgtttt tcctgcccga cacgccacca aaaagcaccg gcaaaatgga tattaaagtc
    69721 atgctcggcc tggatgcgct gatcctgctg cgcgataaaa acttcctcgt ctttttcttc
    69781 tgttcattcc tgtttgcgat gccactggcg ttctattaca tctttgccaa cggttatctg
    69841 accgaagtcg gcatgaaaaa tgccaccggc tggatgacgc tcggccagtt ctctgaaata
    69901 ttctttatgc tggcattacc gtttttcact aaacgctttg gtatcaaaaa ggtattattg
    69961 cttggtctgg tcaccgctgc gatccgctat ggcttcttta tttacggtag tgcggatgaa
    70021 tatttcacct acgcgttact gttcctcggt attttgcttc acggcgtaag ttacgatttt
    70081 tactacgtta ccgcttacat ctatgtcgat aaaaaagccc ccgtgcatat gcgtaccgcc
    70141 gcgcaggggc tgatcacgct ctgctgccag ggcttcggca gtttgctcgg ctatcgtctt
    70201 ggcggtgtga tgatggaaaa gatgttcgct tatcaggaac cggtaaacgg actgactttc
    70261 aactggtccg ggatgtggac ttttggcgcg gtgatgattg ccattatcgc cgtgctgttc
    70321 atgatttttt tccgcgaatc cgacaacgaa attacggcta tcaaggtcga tgatcgcgat
    70381 attgcgttga cacaagggga agttaaatga aaacagaacg tattctcggt gctctttatg
    70441 ggcaggcgtt aggggatgcg atggggatgc cctccgagct ttggccacgc agccgcgtta
    70501 aagcacactt tggctggatt gaccgttttc ttcctggacc aaaggagaat aacgcggcct
    70561 gttattttaa ccgcgccgaa ttcaccgacg atacctcgat ggcgctgtgt ctggcggatg
    70621 cgttactgga acgtgaaggc aagatcgatc cgaatctgat tgggcgtaat attctcgact
    70681 gggcgctgcg tttcgacgcc tttaacaaaa acgtacttgg tccgacctcg aagattgcgc
    70741 ttaacgccat tcgcgacggt aaacccattg ctgaactgga aaacaacggc gtgaccaacg
    70801 gcgcagcgat gcgcgtctcg ccattaggtt gtttgcttcc ggcgcacgat gttgattcct
    70861 ttattgatga tgtggcgctg gcgtccagcc cgacccataa atccgatctg gcgattgcag
    70921 gcgcggtagt catcgcatgg gcgatttctc gtgccattga cggagaaagc tggtcagcga
    70981 ttgtagattc actgccttca attgcgcgac atgcacagca aaaacgcatc actaccttca
    71041 gcgcctcact ggcagcacgt ctggagattg cgctgaaaat tgtgcgcaat gccgacggca
    71101 ccgaatccgc cagcgaacag ctttaccagg tcgttggcgc aggtaccagc actattgagt
    71161 ccgttccgtg cgccattgcg ctggttgaac tggcacaaac cgacccgaat cgctgcgccg
    71221 tcctgtgcgc taaccttggc ggcgacacag acaccatcgg cgctatggcg acggcgattt
    71281 gcggcgcgtt gcatggcgtt aacgctatcg atcctgcatt aaaggcggaa ctggatgcgg
    71341 taaatcagct tgatttcaac cgctatgcca cagcgctggc gaaatatcgt caacaacggg
    71401 aggcggtatg agcggcgctc gattacacac gctgctgcct gaattaacct cgcgtcagcc
    71461 ggtgatggtc gtcggcgcgg cggtcattga tgtgatcgcc gacgcttatg ccctcccctg
    71521 gcgtgggtgc gatatcgaac tgaagcagca gagcgttaac gttggcggct gcgcactgaa
    71581 tattgccgtg gcgttaaagc gcctcggcat cgaagcgggt aatgccttgc cgctcggtca
    71641 gggcgtgtgg gcggagatta ttcgcaaccg gatggcgaaa gaggggttaa tcagcctgat
    71701 cgataacgcc gaaggcgata acggctggtg tctggcgctg gttgagccgg atggcgaacg
    71761 cacttttatg tcattcagcg gtgtggaaaa tcagtggaat cgccagtggc tggcgcgatt
    71821 aaccgttgcg cctggcagcc tgctctattt ttccggttat caactggcct cgccctgcgg
    71881 cgaattgtta gtggaatggc tggaaaagct gcaagacgtg acgccgttta tcgattttgg
    71941 cccacgtatc ggcgatatcc cggacgcatt actggcgcgg atcatggcct gtcgaccttt
    72001 agtatcactc aatcgtcaag aggctgagat tgccgccgaa cgttttgctt tatccgcaga
    72061 gataacaaca cttggcaagc aatggcagga gaaatctgcc gcgccgttga tcgttcgtct
    72121 cgataaagag ggtgcatggt atttcagcaa cgacgcttct ggctgcattc cggcatttcc
    72181 aacgcaagtt gtagacacca ttggggcggg cgacagtcat gcaggtggcg tacttgccgg
    72241 gctggcctct ggtctgccac tggcggatgc cgtattactg ggcaatgcag tggcgtcgtg
    72301 ggttgtcggg catcggggcg gtgattgtgc gccaacgcgc gaggaactac tcctcgcaca
    72361 caaaaacgta tagatcgctg cgacagtggc taatgctgta ctcaataggc cgttgctgtt
    72421 ggtcaagcgc cacttgcttg atcaccagca ccggtatttt gctatctaac tgaatatgtg
    72481 actgaaactc ggcatccggc atccgggcgc taacgcggga acgtgtacgt tgtgggtaaa
    72541 tgtgctggct gcggaaatag tcataaagtg aaatcccgat ggcatcaaca tcatgaatta
    72601 aatgcgccgg aacccacgat tcctcaatcg ataccgcttc ttcatcgaca tagcgaatac
    72661 gcttaagcaa aaagacatca cttcctgctt cgaccgccag ctgctgcgcg acttcttcgg
    72721 gacatttcac gacacgcttg ttaacccata gcgtatcggg ctttttcccg cgcaatacca
    72781 cctgctgaga aaaacctcgc gcttctttca acgagtattc gaagatgttg ttgatctgcg
    72841 tgccgtaacc gcgcgaacgc gtcaccacac cctcttcttc cagcgcctgc atcgccttgc
    72901 gcaccgtaat gcgcgaaacg ccggttaact gacttaaatc acgctcaccg ggcaaaatat
    72961 tgccatgctc cagcaccccg ctgcgcacgg cattttttac cgtttcggca aacttaatat
    73021 aaagcggcgt gttatccgca gctaaaatac gctcgttcag ttgggcaatc agctgggtat
    73081 gcgcttgttc catttgtctt tttcctgcca gggagtctgg tgggagtata tcactgcttc
    73141 ttatttaagg cgtttcttta atcccatcaa cgaatccctg caactcctcc accgtgccat
    73201 taaacacatt aaaatccacc ggaccattga tgccatctac ctgtccacgg tcggaatgct
    73261 gccagaagcg ccaggccatt ccgtcattgt ccggacgacg ttgataatag tgagccaccc
    73321 accacggata ttcattaaaa tagcccgcca gattcgtgtg ataaaaaacg gctcctgagt
    73381 aaataatcgg cttttttccc gtgcgttttt ctaccatttt tagccactga cttacccgct
    73441 tgcgtaattc ttttgccgat aattttcccc gttcttctac gtccagcacg gcagggaaat
    73501 cgccttgtga gaaatccacc gtttgcagaa ataatcgcgc ctgaactgaa gcggataccg
    73561 acggggagaa ataatgatac gccccacgca gcatgccatt ttcgcggctt agttgccagt
    73621 tacgcgaaaa ataggggtcc accagctttt cgccttccgt cgccttaata aaagcaaact
    73681 gtaagcggat accgttgtcg cgcatttttg ccacccgctg ccagtcgatc cgctcctgcc
    73741 agcgcgaaac atctattccg tgaatggtat aactggcagg aatgcgaatt gcgaaagatt
    73801 taaccgggcg ataaccgtag aagtggatgt agtctttaat ctgaactgct gtcgaataga
    73861 aaaaattcac cgtctgacgc ggataaattg caatgatgga aattagccca agaacgcata
    73921 ataaagcggt gaattttttt cgactggtga ttctcagctg cattggtttc atccctgaat
    73981 gtcagtgcca aagactgaca ataaccaaag cagctatagt acggtgctca ccaccaggcg
    74041 tggaagtggt gaaccggacc aataccgtga ccaacttcca gcgtgtcggc ctgggctaac
    74101 gccgatgaaa gccagctttt tgcctcctgt acggtgtcag cccagtttgt atggcgcggg
    74161 cgtagtgcag ccaacgccgc agagagtgta caaccagtgc cgtgggtgtt tttggtcata
    74221 atgcgcggtg cggtaaaccg ttgttcaccc tcgcgagtaa acagccagtc cgggctttgc
    74281 tcatcatcca gatgaccacc tttcattagc actgcgccac agcccatcgc caacagcgat
    74341 cgcccttgtt ccagcatttc ctgttcgttg cgcgcgtgtg gcgcgtcgag caaggcggca
    74401 gcttcgggca agtttggcgt tattaatgaa acctgtggca ataatcgact gcgcagcgta
    74461 gcaaccgccg aaggtgaaag cagcggatcg ccgctttttg ccagcataac agtgtcgagt
    74521 accacgtttt ggatctgata acgttgcaac cgttctgcca ccgcttcaac aatatcggtt
    74581 tccgccaaca taccgatttt agtggtatcg attcgcacat cgctgaacac cgaatcgagc
    74641 tgggcggcga caaaatcagg ctcaatgcga tacaccgact gtacgccacg ggtattttgc
    74701 gccaccagtg cagtaataac tgagcaacca taagcgccaa gtgccgagaa ggttttaaga
    74761 tcggcctgaa tccccgcacc accactcgga tcagtaccgg caatcgtcag agcgttaatt
    74821 cgtttcatgc ctgcacctcc tgcgtcaatt gccagagcgc atcaaggaaa tgtggaacaa
    74881 aactgcctgg cccctcgctt ctggcgacag cgcgttctcc ggcttgtttc atccagtgac
    74941 aggcagatgc gacattttcc agcatatcgc ctggtaaggc acaacaggca gcgacaaccg
    75001 ccgataatgc acagccagtt cctaccactt tggtcattaa cggatcgcca ccgtgaatac
    75061 caatgatacg atgtccatcg gtaacataat ccacctcgcc agtgaccacg acgattgcgc
    75121 cagtttcccg tgccagtgtt tgtgcagcgg gtatcgcgtt agctgcggcg tcagtggtat
    75181 ccactccccg tccgccatta gcaatgccag ctaatgccat gatttccgaa gcattaccac
    75241 gtatcgctgc cggtttaaaa gataaaagtt catgacaaaa atggcggcga taatcgagcg
    75301 cacccaccgc tactggatca agcgtccagg gtgtttgaga gctttttgct tgctcaacgg
    75361 cagcacgcat cgcctgtgcg cgtggctgcg tcagtgtgcc aacgttaatc aacaaggcac
    75421 tggcgatagc cgcaaactga ctggcctctt cggtttcgat aaccatcgct ggcgatgcac
    75481 cgagcgccag caaggtattg gcggtaaagg tttgcaccac atcattggtc atgcagtgca
    75541 caagagggga atgttggtga aaaaggtgta acgcgtgcgc agattgcgct gaacccagca
    75601 ggtcgacttg catagtttgc tcctgccata acgtgaagaa gcaatgacct ggtggtccgt
    75661 gacttcccta cgctggcatt atccagatca ggtgatacgg gtatttctca gccttcacgc
    75721 agaagggcac cccgagtcgt ttggttgcga tgataaatcg cagaggagga tggtaatgtc
    75781 cagcgcacgc gttgtaaacg aggaaaaacg gcagttttac aatcgcgtta ataaattatt
    75841 tgatatatga atccagcacc ttcagaacga catccagatc ttcttcacgt tttagctcat
    75901 ccccctggtg aacgatgtgt tccgtcagat gacctttaat cacttcccgc atcagaccgt
    75961 ttaccgcgcc acggatagca gcaatctgtt gtaaaactgc agcgcattcg tgcggctcgt
    76021 cgagcatttt cttgagcgcc acgacctggc cctgaatctt actggcacgc gctttcagtt
    76081 tctgtttatc acggattgta tgagacatgg caacacctgg ttaacaagaa tatgaaaaat
    76141 catagcacta ttaatctact ggggggtagt atcaggtact gggggggagt agaatcagat
    76201 tgccgaatta atactaagaa ttattatcat gacagaattt acaactcttc ttcagcaagg
    76261 aaacgcctgg ttcttcatcc ccagcgccat cttacttggt gcgcttcatg gcctggaacc
    76321 agggcactca aaaacgatga tggcggcgtt tatcatcgcc atcaaaggca ccattaaaca
    76381 ggcggtgatg ctcggactgg cagcaaccat ttcgcatacc gcggtggtct ggttgattgc
    76441 ctttggcggg atggtgatca gtaagcgttt tactgctcaa tcagcagaac cgtggctcca
    76501 gctgatttcc gcagtgatca ttattagcac cgcgttctgg atgttctggc gtacctggcg
    76561 cggcgaacgc aactggctgg agaatatgca cgggcatgat tatgagcatc atcatcacga
    76621 tcacgaagat caccacgacc atggacatca tcaccatcac gaacatggcg agtatcagga
    76681 tgcccatgca cgagcccatg ccaatgatat taaacgacgc tttgatggta gagaggtcac
    76741 caactggcaa attttgttat ttggcttaac aggtggtctt atcccctgtc cggcagcaat
    76801 taccgtgctg ttgatttgca ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt
    76861 cagtttcagc attggcctgg cgttaacgct ggtcaccgta ggcgttggcg cagcaatcag
    76921 cgttcagcag gtcgcaaaac gctggagcgg atttaacact ctcgctaaac gcgcccccta
    76981 tttttccagt ctgttgattg gcttagttgg tgtgtatatg ggcgtacatg gcttcatggg
    77041 cataatgcga taactcaatc ctttaggctt gcatgttatc ttcagcccag gatgattctt
    77101 aaatcagcta tttccgctga ctctctgctc gctaaggatg cttttagggc atcctttcat
    77161 ttacactttt tacgaaatca tgggatcact aacaaaatat cgcttgtcag ttatattgta
    77221 tggcaggaaa gatatgcgac tgatattaca gatccccaaa gtggagagtt tatgaccatt
    77281 aaaaataaga tgttgctggg tgtgcttttg ctggttacca gtgccgcctg ggccgcacca
    77341 gccaccgcgg gttcgaccaa tacctcggga atttctaagt atgagttaag tagtttcatt
    77401 gctgacttta agcatttcaa accaggtgat accgtcccag agatgtaccg caccgatgag
    77461 tacaacatta agcagtggca gttacgtaac ctgcccgcgc ctgatgccgg gacgcactgg
    77521 acctatatgg gtggcgcgta cgtgttgatc agcgacaccg acggtaaaat cattaaagcc
    77581 tacgacggtg agatttttta tcatcgctaa aaaaatcccc ctcatcatga gggggaaatg
    77641 cagacacctt gttatttttt attattagcc acttgctcgt cttgcttgtt attagtcgta
    77701 tttcacgttg attaatgcgg ttgcctccag tgcgccagat ttaactttgt ttgtatcgta
    77761 gacgtagtaa ctggctgtta tcggaattga aaccgttgta tctgtaagtt ttgactttgc
    77821 cgcaatttct tttaaatttg tgtatccttt gctgttatca gtgatattta aaagtgtgac
    77881 tggcgaacca ttactttcaa gaataaatcc tacgccatta tcttccgcac ctttcagtat
    77941 tgtgttgctg ttggttaaca agtcagaact tgataaatac acctgcattt tccgtgatgt
    78001 ttgattttgt tgacctaata aatttttaca acgcagaata atatcccctt ttttactatt
    78061 tattgttgct ttgttacctg tggctggtaa ttgtgaaacg ggaataccct ctaaagtaat
    78121 taataaatcg tccggtttgc aggttgtggt tatcgggttg tatgacaagg tcagattaaa
    78181 ggaatagaca cctgcatttt gtgccaatgc atttctatag ctatttacac atgcatccca
    78241 tgacttgcca cctaataaac acagtagaaa ttgtactgcg gcactcccgc gtgtcgatga
    78301 tgaagcactg gataatgtcg caacaccatt aatcatcact gaaccgccgc cccccgcaga
    78361 aacattcact tttgcattac tggccgcagg tgtcaccgtt accgaataac cattggtgaa
    78421 actggtctga ttatttgaca acgtgatgtt atttttattg gtccattcaa aatgtaaatt
    78481 taatttaatg gtatcgttat aaagtccaac aataatatcc gacgggctca taggggtgat
    78541 agtatttgta ctcacgcatg acgaatcatt aaaattatgc tcatatgtcg ccatttgtgt
    78601 ttgagtaagt gcaaaatccc cctgaacaac caagttgtgc tgaacagata actcactata
    78661 acatgccgca tagctcacag gcataaccat catcattaat ataaatagca ttatgcgaat
    78721 ttccatagaa ccacctgact attactggca aatataattt ctactttcat caatctcttt
    78781 accgaaggtg attgtgcatg aaagtccttg ttgcttatca attgctacat taaccgatgg
    78841 cggtacttca ttggtgcgaa taaataactg gcttccctga ccgacaacgc caatattatg
    78901 accatggata tcattgactt cataaccaaa cattaatggt tgcccatccg ctcttaacgc
    78961 ttttataaac cagggcttgc gctgatcggt atcaaaatta accagtacaa ccgcgccgcg
    79021 ataaggggcg gcaattttcc ggttgccacg taattctgct tcgctatcgc tttgcgacac
    79081 atccagcatc aggtgatttt cccgataagg tgtcattccg tcgtatacca ccactccatt
    79141 acggtttgtt gtgcgatatt tttgcccatt gacataagca tctttaattc ctggggcatt
    79201 catcacagca aacgtttcgg aaagacggtt cgccagatta acgccccccg accaggcgac
    79261 aatgccccca gaaacactgg ctccagcctg tcgataagta ctcgactgac tataactgcc
    79321 attcactgtc gcaaccggcg cgttccaggt caaattcgcc ccagctgtcg tttcatttcc
    79381 ctgatgctga taactcaggt tgaccccata gttaaactga tcccggcttc caacggttcc
    79441 tgataatccc gtattatttg aggcaaaccc ctgatcatca aacgtcgttg agttagacat
    79501 atatatttgc cgacgaggcg tcgtaacgtc atcaccccaa tcaaagggaa tcgatataaa
    79561 aatattaaat cgtttctctt catgatgatt ctcgtcataa gcctggcttg ccgcgagggt
    79621 atagcttatc cgccgccagt tattggaata actcaactga taatccttac tgctgccgct
    79681 acgtccccag taatctcgcc ataacgtact taaggacaca gaaccccagc cttctggcaa
    79741 tgactggctc atattggcag aaaagctatt tttacggcca aaatcgttct ggtaataatc
    79801 ggcaatgtca tagacatcgt tttcatcacg gcgataatta tctttattgt ttgcccaaac
    79861 gtgatcatta aatgtccggt aatcacgcga tgaataacgc caggccgcaa gaccaaaacg
    79921 cgtcgacgtt tggctcacaa atttgttgta ggcaatttga taactttgcc cgtcaaacac
    79981 atcgccgttg tcttgtttac tatgcgactt agtggcatcg acggaaatgg caccaatgcg
    80041 tgtattccag ccagtcccca aagtaaacgc gtaataatta ttcgcgacca tcgagccgcc
    80101 atacagcgtc aataaattat taaaaccata ctgataaccc acctggacaa attcactttg
    80161 tttgctcgcc ccttcaatat ggctacgacc cgccgcaaaa tcatatttcg acacgccggg
    80221 ttgcagcata tttggcaccg ctgcataagg caccagatag gtggttaccg aaccgtccgc
    80281 ctctttcacg ctgacatcaa gatctgctcc accaccggcc aactgcaaat ctgtaatcgc
    80341 gaacgggcca ggaggaacct ctttctgata aacgacaaaa ccattctgtt caatagttac
    80401 cagcgcgtta ctctgagcaa tcccctgcac ccgtggcgta aaattttgtt tcgagttagg
    80461 caacatctgc atatcacgaa acaaccgcac acctctgaag cgaacagaat caaaaatatc
    80521 gcttgatgtg tacatatcac ccacgcgaag cgtgccgaga agttgggcaa atccacgttc
    80581 cagatacagg gtattgcttt tccacacccc tggattgtta tttgttttac tgaaactggc
    80641 atcagaatgc agttgccacc ccagtaaatt taacccgctg ttaaaacgta catatgtact
    80701 cttgttatta cccgacgctt tatagtcgct gtaatactga ctcagataat aagaggtata
    80761 aaacgcatta ataccccgct cccagttttc cggtggaaca tagccacttt ccagttcttc
    80821 cacccaggcc tgcgggacac tgaaatcgag acgaaaaacc ccgatatccc aggtatagct
    80881 cccaccctga acaagttgct caaatgttaa acattgctta ccgctggcga agttatcgct
    80941 attaatgcct aaccgcttga taacttctct tgataaacat gtttcttgcg ggttgtcttt
    81001 aacaataatc tcatatttcc cgcgccattg cttattgaca taaatatcga tgtcatactg
    81061 ccccggtaag ggttgattat catcaagacg aatatttgca acctgctggt ctttcattcc
    81121 acctatcata aaatgggtat caaaggtttc ttcagctgca taagcttcaa tgccgagcaa
    81181 taacgctacg attgctgatg caagtggggt cattctcaac ataggcagct cctgcaatta
    81241 aattttgtca ctaatatagt tgccatggtc atcgataatg gtcagatgcc agttatttgc
    81301 attattactt ttgacattaa cactctgact gtctaagggg gcaatcataa tagtttcata
    81361 attgactttg acattattag ctttgacatc cgaaatcgtc acccaattag ctgagtcatt
    81421 ttttatcacc aaaccattgc cactgcgatt taccagcaat tttttaaatg tcgctttatt
    81481 taccggagca atacccgctg gccggtaaaa caacttaatt ctgttttgca tcgcaaactt
    81541 cagtgcattc ttaccttctt gctctggact atttggtgga atatccagaa cattcagata
    81601 aaaaatgctt tctttattag tgggcagttt attcggcata attttgattt ttacttgctg
    81661 cccggaattt gcccctattt ttgccactgg tggcgttaac atgaaaggaa cctgaatttt
    81721 ttctggtggt aatgacgtat cgccatcatc aatccacgcc tgcagcagcg aagaacggtt
    81781 tccctggttc atcaactgca ccatcacttc tttattttct gccgggtaaa taatgcgcgt
    81841 cccgtagata acaattccgg catgtgcagg aaggaccata gaaaaaatga gtaaagataa
    81901 taatcctttc attttaatgc ctcttaaatt aaaaggccgc catgggatag cggccatgtt
    81961 tattacctgg agaaacttac aattttattt attaatcctg cgccacacgc ataaccagag
    82021 aagataacac atcacctgat tgcactgagt tatctgtcag tttcaggtat tttgctttaa
    82081 acggaatagc atattgacct tccgctgttt tataactctc aatgttaaaa ggtgcagaaa
    82141 catccaggac atcgctgtta tcacctaaat agagatagta acccacggta tcgttgctac
    82201 tggctatcgc cttatcgtta ccggttattt tatcgttggt aatcaacgag agttttacac
    82261 cagcaacttc tttcgggcag ctaattttta acatgctgct ggttacaccg actgcatcat
    82321 cataggtaga atatttagct ttaccttcaa agacactggt tggtacagtc ggcatattaa
    82381 tacttactga atcaccaccc gcttcgaatt tacacggaga ttctgcaata ttccccttaa
    82441 tagttaatgt tccggtatca acgtctgcag caaatactcc agcgctcata aaaaaagaag
    82501 aaaagacagc ggcagcaata attgaacgtt tcataatatg ttccttaata aaatttattt
    82561 ttggttgtta agagtctgta tctcttggaa acaaatttct cacatcaaaa aagttgccgc
    82621 aatgtaaaca cattg
//
LOCUS       DBBGLQ010000012        81697 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.012, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000012 DBBGLQ010000000
VERSION     DBBGLQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.012"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            166..675
                     /gene="eco"
                     /locus_tag="ACIYSB_001079"
     CDS             166..675
                     /gene="eco"
                     /locus_tag="ACIYSB_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGC5428171.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(1051..2697)
                     /gene="mqo"
                     /locus_tag="ACIYSB_001080"
     CDS             complement(1051..2697)
                     /gene="mqo"
                     /locus_tag="ACIYSB_001080"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGC5428172.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLNYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(2915..4558)
                     /gene="yojI"
                     /locus_tag="ACIYSB_001081"
     CDS             complement(2915..4558)
                     /gene="yojI"
                     /locus_tag="ACIYSB_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGC5428173.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(4634..5284)
                     /gene="alkB"
                     /locus_tag="ACIYSB_001082"
     CDS             complement(4634..5284)
                     /gene="alkB"
                     /locus_tag="ACIYSB_001082"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGC5428174.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTIDCRYNLTFRQAGKKE"
     gene            complement(5284..6348)
                     /gene="ada"
                     /locus_tag="ACIYSB_001083"
     CDS             complement(5284..6348)
                     /gene="ada"
                     /locus_tag="ACIYSB_001083"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGC5428175.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(6422..7477)
                     /gene="apbE"
                     /locus_tag="ACIYSB_001084"
     CDS             complement(6422..7477)
                     /gene="apbE"
                     /locus_tag="ACIYSB_001084"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGC5428176.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(7589..8692)
                     /gene="ompC"
                     /locus_tag="ACIYSB_001085"
     CDS             complement(7589..8692)
                     /gene="ompC"
                     /locus_tag="ACIYSB_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGC5428177.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGNPSGEGFTSGVTNNGRDALRQNGDGVGGSITYDYEGFGIGGAI
                     SSSKRTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANK
                     AQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            9431..12103
                     /gene="rcsD"
                     /locus_tag="ACIYSB_001086"
     CDS             9431..12103
                     /gene="rcsD"
                     /locus_tag="ACIYSB_001086"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGC5428178.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDNWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTENVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMIFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12120..12770
                     /gene="rcsB"
                     /locus_tag="ACIYSB_001087"
     CDS             12120..12770
                     /gene="rcsB"
                     /locus_tag="ACIYSB_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGC5428179.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(12970..15819)
                     /gene="rcsC"
                     /locus_tag="ACIYSB_001088"
     CDS             complement(12970..15819)
                     /gene="rcsC"
                     /locus_tag="ACIYSB_001088"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGC5428180.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTLYAERVRKSRDS"
     gene            15986..17812
                     /gene="atoS"
                     /locus_tag="ACIYSB_001089"
     CDS             15986..17812
                     /gene="atoS"
                     /locus_tag="ACIYSB_001089"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HGC5428181.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            17809..19194
                     /gene="atoC"
                     /locus_tag="ACIYSB_001090"
     CDS             17809..19194
                     /gene="atoC"
                     /locus_tag="ACIYSB_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HGC5428182.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            19390..20052
                     /gene="atoD"
                     /locus_tag="ACIYSB_001091"
     CDS             19390..20052
                     /gene="atoD"
                     /locus_tag="ACIYSB_001091"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HGC5428183.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            20052..20702
                     /gene="atoA"
                     /locus_tag="ACIYSB_001092"
     CDS             20052..20702
                     /gene="atoA"
                     /locus_tag="ACIYSB_001092"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HGC5428184.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            20699..22021
                     /gene="atoE"
                     /locus_tag="ACIYSB_001093"
     CDS             20699..22021
                     /gene="atoE"
                     /locus_tag="ACIYSB_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HGC5428185.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            22052..23236
                     /gene="atoB"
                     /locus_tag="ACIYSB_001094"
     CDS             22052..23236
                     /gene="atoB"
                     /locus_tag="ACIYSB_001094"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HGC5428186.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SQHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKNLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(23310..24086)
                     /gene="yfaP"
                     /locus_tag="ACIYSB_001095"
     CDS             complement(23310..24086)
                     /gene="yfaP"
                     /locus_tag="ACIYSB_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGC5428187.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(24091..25740)
                     /gene="yfaQ"
                     /locus_tag="ACIYSB_001096"
     CDS             complement(24091..25740)
                     /gene="yfaQ"
                     /locus_tag="ACIYSB_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HGC5428188.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVNPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQAETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(25741..30255)
                     /locus_tag="ACIYSB_001097"
     CDS             complement(25741..30255)
                     /locus_tag="ACIYSB_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGC5428189.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     KQFRYPLWQAKPFEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     IGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALAKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(30279..30897)
                     /gene="yfaT"
                     /locus_tag="ACIYSB_001098"
                     /pseudo
     CDS             complement(30279..30897)
                     /gene="yfaT"
                     /locus_tag="ACIYSB_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
     gene            complement(30894..32582)
                     /gene="yfaA"
                     /locus_tag="ACIYSB_001099"
     CDS             complement(30894..32582)
                     /gene="yfaA"
                     /locus_tag="ACIYSB_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGC5428190.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAITGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARWLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGIAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(32731..35358)
                     /gene="gyrA"
                     /locus_tag="ACIYSB_001100"
     CDS             complement(32731..35358)
                     /gene="gyrA"
                     /locus_tag="ACIYSB_001100"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGC5428191.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            35505..36227
                     /gene="ubiG"
                     /locus_tag="ACIYSB_001101"
     CDS             35505..36227
                     /gene="ubiG"
                     /locus_tag="ACIYSB_001101"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGC5428192.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(36355..40107)
                     /gene="yfaL"
                     /locus_tag="ACIYSB_001102"
     CDS             complement(36355..40107)
                     /gene="yfaL"
                     /locus_tag="ACIYSB_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGC5428193.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQ
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNATVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYA
                     WFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(40532..40675)
                     /gene="ypaB"
                     /locus_tag="ACIYSB_001103"
     CDS             complement(40532..40675)
                     /gene="ypaB"
                     /locus_tag="ACIYSB_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGC5428194.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            40803..43088
                     /gene="nrdA"
                     /locus_tag="ACIYSB_001104"
     CDS             40803..43088
                     /gene="nrdA"
                     /locus_tag="ACIYSB_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGC5428195.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            43322..44452
                     /gene="nrdB"
                     /locus_tag="ACIYSB_001105"
     CDS             43322..44452
                     /gene="nrdB"
                     /locus_tag="ACIYSB_001105"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGC5428196.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            44452..44706
                     /gene="yfaE"
                     /locus_tag="ACIYSB_001106"
     CDS             44452..44706
                     /gene="yfaE"
                     /locus_tag="ACIYSB_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGC5428197.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(44760..45410)
                     /gene="inaA"
                     /locus_tag="ACIYSB_001107"
     CDS             complement(44760..45410)
                     /gene="inaA"
                     /locus_tag="ACIYSB_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGC5428198.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(45490..45594)
                     /locus_tag="ACIYSB_001108"
                     /pseudo
     CDS             complement(45490..45594)
                     /locus_tag="ACIYSB_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            45589..45831
                     /locus_tag="ACIYSB_001109"
                     /pseudo
     CDS             45589..45831
                     /locus_tag="ACIYSB_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(45873..46949)
                     /gene="glpQ"
                     /locus_tag="ACIYSB_001110"
     CDS             complement(45873..46949)
                     /gene="glpQ"
                     /locus_tag="ACIYSB_001110"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGC5428199.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDNLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(46954..48312)
                     /gene="glpT"
                     /locus_tag="ACIYSB_001111"
     CDS             complement(46954..48312)
                     /gene="glpT"
                     /locus_tag="ACIYSB_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGC5428200.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQERN
                     GG"
     gene            48585..50213
                     /gene="glpA"
                     /locus_tag="ACIYSB_001112"
     CDS             48585..50213
                     /gene="glpA"
                     /locus_tag="ACIYSB_001112"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGC5428201.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            50203..51462
                     /gene="glpB"
                     /locus_tag="ACIYSB_001113"
     CDS             50203..51462
                     /gene="glpB"
                     /locus_tag="ACIYSB_001113"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGC5428202.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            51459..52649
                     /gene="glpC"
                     /locus_tag="ACIYSB_001114"
     CDS             51459..52649
                     /gene="glpC"
                     /locus_tag="ACIYSB_001114"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGC5428203.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            52842..53741
                     /gene="rpnE"
                     /locus_tag="ACIYSB_001115"
     CDS             52842..53741
                     /gene="rpnE"
                     /locus_tag="ACIYSB_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGC5428204.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLATTQLTDADIPNCH"
     gene            53742..53939
                     /locus_tag="ACIYSB_001116"
                     /pseudo
     CDS             53742..53939
                     /locus_tag="ACIYSB_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(53980..54783)
                     /gene="yfaU"
                     /locus_tag="ACIYSB_001117"
     CDS             complement(53980..54783)
                     /gene="yfaU"
                     /locus_tag="ACIYSB_001117"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HGC5428205.1"
                     /translation="MNALLSNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     EQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(54801..56089)
                     /gene="yfaV"
                     /locus_tag="ACIYSB_001118"
                     /pseudo
     CDS             complement(54801..56089)
                     /gene="yfaV"
                     /locus_tag="ACIYSB_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(56146..57351)
                     /gene="rhmD"
                     /locus_tag="ACIYSB_001119"
     CDS             complement(56146..57351)
                     /gene="rhmD"
                     /locus_tag="ACIYSB_001119"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HGC5428206.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLSATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYESYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(57366..58148)
                     /gene="yfaX"
                     /locus_tag="ACIYSB_001120"
     CDS             complement(57366..58148)
                     /gene="yfaX"
                     /locus_tag="ACIYSB_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HGC5428207.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(58368..59570)
                     /gene="yfaY"
                     /locus_tag="ACIYSB_001121"
     CDS             complement(58368..59570)
                     /gene="yfaY"
                     /locus_tag="ACIYSB_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGC5428208.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(59670..60212)
                     /gene="yfaZ"
                     /locus_tag="ACIYSB_001122"
     CDS             complement(59670..60212)
                     /gene="yfaZ"
                     /locus_tag="ACIYSB_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family protein"
                     /protein_id="HGC5428209.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTETT
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            60491..60916
                     /gene="nudI"
                     /locus_tag="ACIYSB_001123"
     CDS             60491..60916
                     /gene="nudI"
                     /locus_tag="ACIYSB_001123"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGC5428210.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWAISGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(60955..61557)
                     /gene="ais"
                     /locus_tag="ACIYSB_001124"
                     /pseudo
     CDS             complement(60955..61557)
                     /gene="ais"
                     /locus_tag="ACIYSB_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
     gene            61865..63004
                     /gene="arnB"
                     /locus_tag="ACIYSB_001125"
     CDS             61865..63004
                     /gene="arnB"
                     /locus_tag="ACIYSB_001125"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGC5428211.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALKIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADHVITALQQLAGQ"
     gene            63008..63976
                     /gene="arnC"
                     /locus_tag="ACIYSB_001126"
     CDS             63008..63976
                     /gene="arnC"
                     /locus_tag="ACIYSB_001126"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGC5428212.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            63976..65958
                     /gene="arnA"
                     /locus_tag="ACIYSB_001127"
     CDS             63976..65958
                     /gene="arnA"
                     /locus_tag="ACIYSB_001127"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGC5428213.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPDVIFSFYYRHLIYDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            65955..66845
                     /gene="arnD"
                     /locus_tag="ACIYSB_001128"
     CDS             65955..66845
                     /gene="arnD"
                     /locus_tag="ACIYSB_001128"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGC5428214.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            66845..68497
                     /gene="arnT"
                     /locus_tag="ACIYSB_001129"
     CDS             66845..68497
                     /gene="arnT"
                     /locus_tag="ACIYSB_001129"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGC5428215.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            68494..68829
                     /gene="arnE"
                     /locus_tag="ACIYSB_001130"
     CDS             68494..68829
                     /gene="arnE"
                     /locus_tag="ACIYSB_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGC5428216.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            68829..69215
                     /gene="arnF"
                     /locus_tag="ACIYSB_001131"
     CDS             68829..69215
                     /gene="arnF"
                     /locus_tag="ACIYSB_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGC5428217.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(69209..69475)
                     /gene="pmrD"
                     /locus_tag="ACIYSB_001132"
     CDS             complement(69209..69475)
                     /gene="pmrD"
                     /locus_tag="ACIYSB_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGC5428218.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(69585..70940)
                     /gene="menE"
                     /locus_tag="ACIYSB_001133"
     CDS             complement(69585..70940)
                     /gene="menE"
                     /locus_tag="ACIYSB_001133"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGC5428219.1"
                     /translation="MIFSDWPWRHWRQVRGETIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(70937..71899)
                     /gene="menC"
                     /locus_tag="ACIYSB_001134"
     CDS             complement(70937..71899)
                     /gene="menC"
                     /locus_tag="ACIYSB_001134"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGC5428220.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(71899..72756)
                     /gene="menB"
                     /locus_tag="ACIYSB_001135"
     CDS             complement(71899..72756)
                     /gene="menB"
                     /locus_tag="ACIYSB_001135"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGC5428221.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(72771..73529)
                     /gene="menH"
                     /locus_tag="ACIYSB_001136"
     CDS             complement(72771..73529)
                     /gene="menH"
                     /locus_tag="ACIYSB_001136"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGC5428222.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWVQRFLTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(73526..75196)
                     /gene="menD"
                     /locus_tag="ACIYSB_001137"
     CDS             complement(73526..75196)
                     /gene="menD"
                     /locus_tag="ACIYSB_001137"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGC5428223.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(75285..76580)
                     /gene="menF"
                     /locus_tag="ACIYSB_001138"
     CDS             complement(75285..76580)
                     /gene="menF"
                     /locus_tag="ACIYSB_001138"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGC5428224.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(76659..76964)
                     /gene="elaB"
                     /locus_tag="ACIYSB_001139"
     CDS             complement(76659..76964)
                     /gene="elaB"
                     /locus_tag="ACIYSB_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGC5428225.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(77019..77480)
                     /gene="elaA"
                     /locus_tag="ACIYSB_001140"
     CDS             complement(77019..77480)
                     /gene="elaA"
                     /locus_tag="ACIYSB_001140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGC5428226.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            77545..78462
                     /gene="rbn"
                     /locus_tag="ACIYSB_001141"
     CDS             77545..78462
                     /gene="rbn"
                     /locus_tag="ACIYSB_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGC5428227.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            78650..79857
                     /gene="elaD"
                     /locus_tag="ACIYSB_001142"
                     /pseudo
     CDS             78650..79857
                     /gene="elaD"
                     /locus_tag="ACIYSB_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
     gene            complement(79928..81655)
                     /gene="yfbK"
                     /locus_tag="ACIYSB_001143"
     CDS             complement(79928..81655)
                     /gene="yfbK"
                     /locus_tag="ACIYSB_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HGC5428228.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPQPENKESQQQQPSTPTEQQVLAA
                     QQAAIKEAEQSAAAAKALAQQEVQQYSDKQALQGRLQEAPTFARAAKAKATHIANPGT
                     ARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYF
                     PSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLV
                     FLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAE
                     INAAIDSLDAEGSTNGGAGLELAYQQATKGFIKGGINRILLATDGDFNVGIDDPKSIE
                     SMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQ
                     MLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRVEHFNNDNVDAGDIGAGKHITLLF
                     ELTLNGQKASIDKLRYAPDNKLAKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTIN
                     APSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIEL
                     ADGVTDISQ"
BASE COUNT        19985 a        21836 c        20035 g        19841 t
ORIGIN      
        1 aattgattca ccagagatat ttctgctggt ttgctctctc attagaattt aacactaaaa
       61 gagcaggtaa aattgtctga atgttcttta agttattcat aaagcaaatt aataaatctg
      121 atgaatatgt taaccttcag cgacatcatc ggtgaaaacc tataaatgaa gaaggaaagc
      181 aaaaaaatga agaccattct acctgcagta ttgtttgccg ctttcgctac cacttccgcc
      241 tgggcggcag aaagcgtcca gccactggaa aaaatcgcgc cttatccaca agctgaaaaa
      301 gggatgaagc gtcaggtgat tcagttaacc ccgcaagaag atgaatctac cctgaaagta
      361 gaactgttaa tcggtcagac gctggaagtc gattgcaatt tgcatcgtct cggcgggaag
      421 ctggaaaaca aaacgctgga aggctggggc tatgattatt atgtctttga taaagtcagt
      481 tccccggttt caacgatgat ggcctgcccg gatggcaaga aagagaagaa atttgtcacc
      541 gcgtatctgg gcgatgctgg aatgctgcgt tacaacagca agctgccgat cgtggtgtat
      601 acgccagaca atgtagatgt gaagtaccgc gtctggaagg cggaagagaa aattgacaac
      661 gcggtagttc gctaaactgc cgtgaagtgc ggcaccccgt aggtcagaca aggcggtcac
      721 gccgcatccg acatccaacg cccgagccgg ttgcctgatg cgacgctggc gcgtcttatc
      781 aggcctacac cgctgtgaag tgcggcaccc cgtaggtcgg ataaggcggt tacgccgcat
      841 ccgacatcta acgcccaagc cggttgcctg atgcgacgct ggcgcgtctt atcaggccta
      901 caccgctgtg aagtgctcca ccccgtaggt tggataaggc ggttacgccg catccaacat
      961 ctaacgcccg agccggttgc ctgatgcgac gctggcgcgt cttatcagca tacgccacat
     1021 ccggcatacc atgccggatg tggcgcatca ttacaacgca atatccgcca cttctttttg
     1081 aacgggttgc ggtttcaact gcggtttcgg cgtactatct gctgcctgcg gcttgtcgta
     1141 gttcaacccc agcacttcgc tggtgtactg caactcgcgt tctgttgccg ctacatcacc
     1201 gttcagcttg cgtccataag acggaacgat cgctttcaac gtagcctgcc attgcgggct
     1261 ggaaacacga tcgccaaata ctttttccag cagattcaac ataatcggcg cggcggttga
     1321 cgcccctggc gatgccccca ggagcgcggc aatggttcct tgctggtcac tgacgacttc
     1381 agtacccaga cgcagtacgc cacctttctc ggcatcacgc ttgataatct gcacgcgctg
     1441 ccccgcttgc cacaaacgcc agtcctcttt tttcgcctgc ggatagtact ctttcaacgc
     1501 ttcaaaacga tcctcttcac tcaacatcac ctgactcacc agatatttca ccagatcgaa
     1561 attatccagc ccgacgtgca tcatcggcat cacgttagag gtggtggtgg aactcattag
     1621 atcccacaat gaaccgtttt tgaggaattt ggttgagaag gtggcaaatg gcccaaacag
     1681 cactacgcgt ttaccgtcca gaacgcgggt atcgatatgc ggaaccgaca tcggtggtgc
     1741 gccaacggat gctttaccgt aaaccttcgc cagatggtga ttaaccacgt ccgggttttc
     1801 cgaaacaagg aactgtccgc ccaccgggaa accggcgtag tctttcgctt ccggaatccc
     1861 cgattcctgt aacagcttca gcgccgcacc gcccgcgccg ataaagacaa atttggcacg
     1921 aatgttctgt gcagtgccat ttttcagatc ggcaacggta acggtccagg tgttatcgtc
     1981 attacgcttt agggcgcgga cttcgctgct gagttgcagc gagaagttag atttcttctg
     2041 caaggaagca attaactggc gggtgatctc gccgtagttc acatcggtac caatttccgt
     2101 acgcgtggct gccactttct gttgcggatc gcgcccttcc atcactaacg gtgcccactc
     2161 tttgatctgc gcgtgatctt cagagtaacg cataccgcga aacagcgagc tttgttgcaa
     2221 cgcggcgtaa cgggcgcgca ggaaattgac gttatcctcg ccccagacaa agctcatatg
     2281 cggaacggta ttgataaatg aacgcggagt acgcagcacg ccgcgctcaa cctggtgcgc
     2341 ccagaactgg cgggaaatct gaaatgcttc gttaatggcg actgcttttt caatactgat
     2401 gctgccatcg gcgttttgcg gggtgtagtt cagttccatc agtgcagaat gcccggttcc
     2461 ggcgttattc cagccgttcg aactctcctg cgcgacaccc tccaggcgct ccaccatggt
     2521 catcgaccat tcaggctcca gctcgcgtaa ataggtcccc aacgtggcgc tcataatgcc
     2581 gccgccaatc aacagtacat cagtttcctg ctcctcggac gctttcgctt ttgccgccat
     2641 cgaaacggca ttaagcccca cggccatcga gaagagcatg gcagtcactt ttttcatctt
     2701 gttaatgcct tacttttagt cgctttattg caggtgagat ttgcgcggca tcaacggtaa
     2761 cacttaagta acaacattta aataatgttt ataaattata ttctaatttt agaaacatta
     2821 taatttcgtt gattaattat agggttatta gaaatgaagg gatttttagc atattgacct
     2881 gacggcagca gtccgccagg tcagcgtcgt aatattatgc cgtccgggca acggcatcac
     2941 gcgaagcggc atcgcgctct tcgcccgtca gctcgctaag ttgcccattg cgcatttcca
     3001 gcaggcggtc ggcgtggata aagtaatgat catcatgact gatagcgaaa atagttttac
     3061 ccatctcctg catcagcggc agcaacacct gataaaactc acgacggaag tgtggatcct
     3121 gatccgccgc ccattcatcc agcaggataa tatcgcgttc ttctgccagc gccagcaaca
     3181 gcgccacgcg ttttttctgc ccttttgata acttcaggtt aacaatacgc ccgttgctta
     3241 actcaagctt atgagccatt ttcagctgcg ccagccactt ctcaaccagt tgcgggttag
     3301 cgggtttacc ctccggcccc agcagttgat caaacagcca gacatcggta aacactgccg
     3361 aaaacagttt gcgataatct tccggttgtt cgccgctgac aggtttgcca tccagcaaga
     3421 tttcgccgct ttgtggctga tacaagcccg tcaacaacat cgccagcgtc gattttccgc
     3481 taccgttgcc gccaatcaga aacagcagct cgccacgttt gatggtgaga ttaatcggac
     3541 caacggaaaa cgcgttatcc tgataagcaa acgtcacgtt acgcagctcc agcgtttgcc
     3601 agttgggaaa cgcctgcggg cgcggaaact ctgctttgaa aggcgcgagc gcgaatttgt
     3661 tcagcttgtt aaacgccacc tgcgccgtca gcagcgtcgg caatgcgcca accgccgaaa
     3721 gcagcggcgt acgcaggaat aaaagcgtca acgaataggt cgcggcaacg ttggtatcag
     3781 cccaaccgag gctgttcgcc atccagaaca ccaggccgat tgcgcccagc atcatgatgt
     3841 ttgaccagtt cacggcacta agatggaagg tgtctgcgcg aataatatgg tggcgatact
     3901 cttgcgcatc aggaatgtag aggttgttaa acacatactc ggcgcgttcc cggttcagag
     3961 tcagctcttt gcgcccttca agtacagttt gaaaatccgt gtacagcttg tcttcggttt
     4021 cacgcagggt cgccatatgt ttgtacaccc gcgccaccag cacaaaaccg ccccagatgg
     4081 tgatcgccat ccagatagcc gttaccagca acattttgcc cgacagcatc cacagatacg
     4141 ccgctgaacc gatagtgaga atgatcccct gcaccagttc cggcagacgc acaaaagcaa
     4201 tggtgatatt gcgcacatcg ctggttaacc ccgccagcaa cgaggcgcta ccgagttgtt
     4261 caatgcgctc gacgtgagta tccagaatcc gcttgataaa ttcactacgc agtcggtaaa
     4321 cgaagtgatg ccccaaagtg gtgagcgcca gttgcgatcc gagagtgact gccatcaaca
     4381 gcagcaataa tcccagaaac tccggcaaca ccagcagact ggtatccgcc gtttcgataa
     4441 ggcgctgatt gataaaagca attaagccaa tgcctaatgc cgcactggcg aggcttagcg
     4501 ccatcacact gataaatggc cagcgatact gccgccagac aagtacaaga agttccatgc
     4561 agaaaacccg gacaatgaat tacagcccgc agtttaaaca tcttcgcgcg cacagcaata
     4621 ataattctta tttttattct tttttacctg cctgacggaa tgtcaggttg tagcggcagt
     4681 cgatggtgag tggatgaaac cccgctttca acggttgaat accgtgataa aacagccgcg
     4741 attcaccgcc ccataccacc acatcgccat gttccaacaa caaacgtttg agcggatcat
     4801 ttcgtttcag gccgccaaat tgaaaaatcg cgggtaagcc cagagaaaca gaaacaattg
     4861 gcgcgcgcag atccggttcg tctttatcct gatgcagcga cagtttcgcg ccaggagcgt
     4921 agcggttgat aagacaagca tctggctgga aatctggata gcccgccgcc gtagccgcac
     4981 gttgacataa attatgaaaa ctctgtggca tggcgggcca cggtttattt gtttgcggat
     5041 caatgggcga atagagataa ccttgccgat gggtcgtcca gcccagatgc ccacagttgg
     5101 tcatcgccac cgacatggta tatcccccgg gggtgaccat ctggcgaaac ggcgactggc
     5161 tggcaacgtc attaatatcg cggatcagtt gctccgcagc gttaaaagca aaacgccgta
     5221 aaattaccgc accagccgcc agtggctctt gccacggttc agcatcggca aacagatcca
     5281 acattacctc tcctcatttt cagcttcgcg gcgcagcagt tgcgctttac gcgacacgcc
     5341 ccagcggtaa ccggaaagtg tgccatcacc acggaccacc cgatgacagg gtattatgat
     5401 agccagcttg ttggcggcac aggcgctggc aacggcccgt accgctttcg gtttgccgat
     5461 ggcgttagcc agttgctgat aactgacggt ttcaccgcaa ggtatcgtgc gcagtgcctg
     5521 ccagacttgt tgctgaaaag cagtgccgcg aatgtccagc ggtaacgtca gcggcgtatc
     5581 gcgttgattg aggctggcga tcacttcacg cacatgttgc tgaaacatca gatcggcagg
     5641 cgcgttgtcg gcagcgggaa acatctgctg caactcgctg attagtgtcg cgtcatcatc
     5701 gcccagcaat atcgcgcaaa tcccccgctc gctttctgcc accaggcaac gacccagctc
     5761 acaatcagcc agcgcgtaac gcaccgccag attttcgcca ccgtgacgga attgtttagc
     5821 cgtcatgccc agcgtttcgt cagctttgcg atagtaactg ctgctgtcgg ggaatccggc
     5881 gttaagaata gacgtcgtca cgctctcccc tttcgccagc gattcgcgca aacggcgagc
     5941 gcgccaggcc tgttgccagg ctttaggcgt cattccggta gtcgctttaa acaaccgatg
     6001 tagatgaaat ggactcatcg ccacctggtc ggctaaggct tccagcgtta caggcgtttc
     6061 ctgttccagc agtcgacacg cgtgggtgat tttatccaac cgatgttgct gggcattggc
     6121 tttttctggc tgacaacgtt tgcaggggcg aaagccagcg gcgagtgcct cgctggcatt
     6181 tgcgtagaag gagacgtttt cccgcaaagc atgtctggcg cggcaagacg gacggcaaaa
     6241 gatgcctgtg gtacgcacgg cgaaaacgaa ttcgccgtcg gcattcgggt cgcgggctaa
     6301 gacagattgc cagcgttgat cgtcagttaa gcatgtggct tttttcataa tcagctccct
     6361 ggttaaggat agcctttagg ctgcccggtc accatcacgc aaaaaccaac aatcttgcgc
     6421 tttaattttt ttcgctgaca aggaagcttt taaactgtgg tgacatccag gttttaaagc
     6481 tatcgccttc tttggtgatc atataaaccg ccagcccttc ccggcgaaca acctctttag
     6541 ctttctccgg cccgagtacc atcaagccag tatcccaggc atcggcttcc agcgccgtcg
     6601 gagcaatcac cgtcacggat accagattgt gttcgatggg acgcccggtt tgcggatcga
     6661 taacatggga aagacgtttg ccgtccagtt cgtaatagtt acggtagctg ccagaggtgc
     6721 taatcccatg accgttgata tccaccacag cctgaaccgc gttttcttta tcggttggtt
     6781 tttgaatcgc tacccgccac ggcaggcctt caccgttcat accacggctg ttcagcgcgc
     6841 cgcccaccga caccagatag cgggaaatcc cttcctgctc catcaagcgt gccaaatgat
     6901 cggccgcata gccttcgccg acggtggaga gatcgacata taaatccggc aggtcttttt
     6961 gcagatattg ctgatgcgac tgattgatga ccgtcaggtg ctgtaagccg gttttggctt
     7021 tcatcgcatc gatctgttcc tggctcggaa tttgaaccgg ctgttgttcc gggccaaagc
     7081 cccacagatt caccagcggc ccgacggtta tatccatcgc gccatcggtc ttcgcgccaa
     7141 tgcgcagcga ggtggtgacg atatcggcca tcgcttcact taccggccac ggcgataaac
     7201 tttgcgagtc gttaaagcgc atcagcgcgg aatctttttt ataggtcgaa agcagctgat
     7261 cgtcggcgtc cagttgggtc tgaatctttt ctttaagttc ggcgctgcgt ttggcgtcaa
     7321 tgcccgggat gctggcacgc cagaaggtac ccatagtttt gccttcaaga acggtaactt
     7381 cagtagcgtg ggttttggcg ggttgtggtt tttgatcgca accaacaaag aagagcgcgg
     7441 cagccagcag tgccacgcgg gtaaagctta tttccatacg tgattatcct catgcgaacg
     7501 gtcgcaagag tacaccaaaa aactgagttt gtacgctgaa aacaatgaaa aaagggcccg
     7561 caggcccttt gttcgatatc aatcgagatt agaactggta aaccagaccc agagctacga
     7621 tgttatcagt gttgatgcca gcgtcacgag tgaactggtt gtcgtccagc aggttgattt
     7681 tgtagtcaac gtaggtggac atgtttttgt tgaagtagta ggtagcacca acatcaacat
     7741 atttcaggat atcttcgtcg tcgtagccac gacccaggtt tttaccttta gactgcaggt
     7801 aagccaggga cggacgcaga ccgaagtcga actggtactg agcaacagct tcgaagttct
     7861 gtgctttgtt cgcccaaccc agggaaccta cgcgagttgc gttgtaggtc tgggtgtact
     7921 gagcagccag gtagatgttg ttagcgtcgt atttcagacc accagtgtag gtttcagcac
     7981 ggtcgccgtt accgatgtaa gcagcggtgt tctgagcatc agtacgtttg gagctggaga
     8041 tcgcaccacc gataccgaaa ccttcgtaat cataagtgat agaaccgccg acgccgtcgc
     8101 cgttttgacg cagtgcgtca cgaccgttgt tagttacgcc actagtaaag ccttcaccag
     8161 atgggttgcc gtttttaccc tggtactgaa cagcaaagtt caggccgtca accagaccga
     8221 agaagtcagt gttacggtag gtcgcgaagc cgttaccacg ctgctgcatg aagttgtcag
     8281 aaccgtaggt gtcaccaccg aattctggca gtacgtcggt ccaggaagtt acgtcataaa
     8341 caacgccgta gttacgaccg tagtcgaaag aacccacatc ctggaatttc agacctgcga
     8401 atgccacacg ggtccaggag ttgttttcgt tttcagcgct gttgccctgg atctgatatt
     8461 cccactggcc gtaaccggtc agctggtcag taacctgagt ttcacctttg aagccaagac
     8521 gcatgtaggt ctggtcgcca tctacatctt tgttgtcaga gaaatagtgc aggccgtcta
     8581 ctttaccgta cagatctaat ttgttgccgt ctttgttgta aacttcagca gcgtttgctg
     8641 cgcctgctac cagcagagct gggaccagga gggacagtac tttaactttc atgttattaa
     8701 ccctctgtta tatgccttta tttgcttttt tatgccactg catactgatt aaccctcatt
     8761 aatcagtcgg caagtccatt ctccccaaaa atgcagaata atccaacacg aatatgatac
     8821 taaaactttt aagatgtttc atttatcgct atagatgttt caaaatgtaa atgcaaggga
     8881 actttttaag attattgcgg aatggcgaaa taagcaccta acatcaagca ataataattc
     8941 aaggttaaaa tcaataactt attcttaagt atttgacagc actgaatgtc aaaacaaaac
     9001 cttcactcgc aactagaata actcccgcta tcatcattaa ctttatttat taccgtcatt
     9061 catttctgaa tgtctgttta cccctatttc aaccggatgc ctcgcattcg gtttttttta
     9121 cccttcttta cacacttttc attattctgt gctaccacag aaaaactata acgcttgtta
     9181 actatttcac aaataattaa catccgcata atttccagca atctttgttt atttgcaatt
     9241 atttttgttg ggctttttgt aggttatttg tacagcaaaa tggcgcttgt acatctattt
     9301 cccccaatgc aggatgataa atatcacggg agaatagaga atcatcaatc aggtaagagt
     9361 ctggaatttt acactgtacc ctttatactg ccctatcact tcgcgaagtt ttaacaggtc
     9421 ataaacacga atgcgtcaga aagagacaac ggccacgacc cgcttttcac tcctaccggg
     9481 gagcattacc cgcttctttt tactgttgat cattgtgtta ctggtgacga tgggtgtaat
     9541 ggtacaaagc gccgttaacg cctggctgaa agataaaagt taccagattg tcgacattac
     9601 ccatgctatc caaaagcgcg tcgataactg gcgttacgtg acctggcaga tctacgacaa
     9661 cattgccgcg acgacctccc cctcctccgg cgaaggttta caagagacgc gcctgaaaca
     9721 ggatgtctac tatctggaga aaccacgccg caaaacggaa gcgttaatct ttggctctca
     9781 cgacaactca acgcttgaga tgactcagcg gatgtccact tatctggata cattgtgggg
     9841 cgcagaaaat gtaccgtggt cgatgtatta cctgaatggt caggataaca gtctggtgct
     9901 gatctcaacc ctacccctta aagatctcac ctccggattt aaagaatcga ccgtcagcga
     9961 cattgttgat tcacgtcgtg cagagatgtt gcaacaggcc aacgccctcg atgaacgcga
    10021 aagcttttct aacatgcgcc gcctggcctg gcagaacggt cattacttta cattgcgtac
    10081 tacctttaac cagccaggac atctggcaac ggtcgtggct tttgatctgc cgattaatga
    10141 tttgatccca ccgggtatgc cgctggacag tttccgcctt gagccagacg cgacggcaac
    10201 gggaaacaat gataatgaga aagaagggac ggatagcgtc agtatccact ttaacagtac
    10261 gaagattgaa atctcctcgg cactcaactc taccgatatg cgcctggtct ggcaggttcc
    10321 ttatggcacc ttattgctgg atacgttgca aaacattctg ctgccactgc tgctgaacat
    10381 cggtttgctg gcgctggcgt tatttggcta taccacattc cgccatttct ccagccgcag
    10441 tacagaaaac gtccccagca cggcggtcaa taacgaattg cgcattttac gggcaatcaa
    10501 tgaagagata gtctcactgc tgccgctcgg cctgctggtt cacgatcagg aatcgaaccg
    10561 cactgtcata agtaacaaaa ttgccgatca tttgctgccg catttgaatc tgcaaaacat
    10621 caccaccatg gcggaacagc atcaggggat tattcaggcg acgatcaata acgagctgta
    10681 tgagatccgc atgttccgca gccaggtcgc gccgcgcaca caaattttca ttattcgcga
    10741 tcaggatcgc gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc tgtatgagaa
    10801 aaaccagcag gggcggatga tctttatgaa aaacattggc gatgcgctga aagaacccgc
    10861 acagtccctg gcggagagcg cggctaaact caacgccccg gaaagcaaac aactggcgaa
    10921 tcaggcagat gtgctggtgc ggctggttga tgaaatacag ttagcgaaca tgcttgcgga
    10981 tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg atgaagttgt
    11041 gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta acaatcatct
    11101 gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt tgctgctact
    11161 gatgcaatat gccgtgacct caacgcaatt gggaaaaatc acccttgagg ttgatcagga
    11221 tgagtcctcc gaagaccgcc tgacgttccg cattctggac acgggagaag gcgtaagtat
    11281 tcatgaaatg gataatttgc acttcccgtt tatcaaccag acccaaaacg atcgctatgg
    11341 caaggcggac ccgctggcat tctggctgag cgatcaactg gcacgtaaac tgggcggtca
    11401 tttaaacatc aaaacgcggg atgggcttgg tacacgctac tctgtgcata tcaaaatgct
    11461 cgcagctgac ccggaagttg aagaggaaga agagcgttta ctggatgatg tctgcgtaat
    11521 ggtggatgtt acttcggcag aaattcggaa tattgtcact cgccagttag aaaattgggg
    11581 tgcaacctgt atcacacccg atgaaagatt aattagtcaa gattatgata tctttttaac
    11641 ggataatccg tctaatctta ctgcctctgg cttgctttta agcgatgatg agtctggcgt
    11701 acgggaaatt gggcctggtc aattgtgcgt caacttcaat atgagcaacg ctatgcagga
    11761 agcggtctta caattaattg aagtgcaact ggcgcaggaa gaggtgacag aatcgcctct
    11821 gggcggagat gaaaatgcgc aactccatgc cagcggctat tatgcgctct ttgtagacac
    11881 agtaccggat gatgttaaga ggctgtatac tgaagcagca accagtgact ttgctgcgtt
    11941 agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg tacccggcaa
    12001 gcagttatgt gaaacgctgg aacatctgat tcgtgagaag gatgttccag gaatagaaaa
    12061 atacatcagc gacattgaca gttatgtcaa gagcttgctg tagcaaggta gcctattaca
    12121 tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc ggtattcgca
    12181 aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac tctacagcac
    12241 tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc tccatgcctg
    12301 gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat ttcccaagcc
    12361 tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg gtattggatc
    12421 tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg aaagctctcg
    12481 ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg ttggaaaaaa
    12541 tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa gttctgcgcc
    12601 tgtttgcgga aggcttcctg gtgaccgaga tcgctaaaaa gctgaaccgc agtattaaaa
    12661 ccatcagtag ccagaagaaa tctgcgatga tgaagctggg tgtcgagaac gatatcgccc
    12721 tgctgaatta tctctcttcc gtgaccttaa gtccggcaga taaagactaa tcacctgtag
    12781 gccagataag acgcgttagt gtcttatctg gcatttgcac cgattgccgg atgcggcgta
    12841 aacgccttat ccggcctacg attcccatta tttcaacaaa ttacattaaa gtaggccaga
    12901 taagacgcgt cagcgtcgca tctggcattt gcactgaatg ccggatgcgg cgtaaacgcc
    12961 ttatccgtcc tacgaatccc gcgatttcct gaccctctcg gcatataacg tcagcgtctg
    13021 ttttatcaca tccagcgtta ccggcttcga caggcagctg tccataccgg actccagaca
    13081 ccgctgcttc tcttcagcca acgcattagc agttactccg attaccggca acgtcagtcc
    13141 caactgacga atgcgttgcg tcaagcggta accatccata tttggcatgt tgacgtcgct
    13201 aagcacgata tcaatatgat tcttgctaag tacattaagc gcatcgacgc catcattcgc
    13261 ggttttacat tgatagccca acgatcccaa ctgatctgcc agcaaacgcc ggttaatcgg
    13321 atgatcatcc acgaccagaa tcatcatatc gtcattatcg ctgaccgctt tgtccgttga
    13381 cggcagagcg ttagcaggat cgtcgctctc catctcgatc aaataaatac gcgccaacaa
    13441 tgccggtagc tcatgcggag cagccacact gtgtacccac tcccctggcg ctttctccag
    13501 cggaatacca atatggcgac gacagaaggt cactaccgct ctgccctgcc attttttact
    13561 cactacctcg tcagtgatca acacatcttc gggagtcggt tcctgccctt cgtatgttgt
    13621 aacgacgatg ccgctgcgct gcaaactggt ttccaggaac tgacagagcg acgcattgcg
    13681 gaccgccagc cagcagcgtt taccactcaa cccttccacg ccttttttct gcgggtactg
    13741 agcgccgtac aacggaatac gcacggtaaa ctggctgccc attcccggtt ctgaatctac
    13801 cgagatatcg ccgtccatca tgctgatcag tttttcacaa atcgccagac ccagaccggt
    13861 cccctggaaa ttacgctgta cgcccgttcc gacctggaag aagggatcaa acaagcgcac
    13921 cacttctttc gccggtatcc ccacgccggt atcgcgaaca cggatagaga gataatcgcc
    13981 atccgcgcga acatgcaaaa ctatacagcc ggtatcggtg aattttatgg cgttactcaa
    14041 caggttggag atgacctgct gtaaacgcat cgggtcgcca tttaaggcca ctggcacatc
    14101 cggttcaata aagcagtaca agcctaactg cttgcgtacc accagcggta aatagttggc
    14161 ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc ggttcgatct tcaactgttc
    14221 cgattcaatc ttcgagaaat cgagaatatc gctgataatt ttcaacaaca ggctggaaga
    14281 gttgttcatt gccgtcacca gccgatcgac gcctttcggt aactctttgg tttgcaacag
    14341 atccaggtta ccgataatgc catacagcgg cgttcgcagc tcatgactga cggtggcaag
    14401 gaacatcgat tttgactggc tcgcctgttc cgctgcttgt gccatctcct gcaacgactc
    14461 ttccatcttc acgcgcgaag aaacatccac cagcacacaa atggccacgt tttcattacg
    14521 atagcgcgaa tggacgaagc tgatttgcag attggtattg ttgctggtca ggacatcaac
    14581 aaaattgacc tgctgcccac agataatttg cgtcagtcgt tggcggtcct catgcgtaag
    14641 catattgaga taggtatgcg ccagttcgtt acttaaaata ttgacgccat cagcggtacg
    14701 caaaatgcag atacccactg gcgcggaggc gacaatcttg cgattgaact gctcatgttc
    14761 ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt acatccgtgc
    14821 gagagtaaac aatgcagctc cggcaagcac attcagcaaa attgcgttaa ggatcaacat
    14881 gcgaatgcgt tccagcacct tatcaaccgg caccgaatac acgatgctta gcgatgaggg
    14941 tggcagattt ttcttcagca ccagctcccg gaacccttcc gtatagccaa accaggagcg
    15001 ttcctgcatc cagcgaggat cgcccttaat tttactttct ggtccggtaa gcgaaatcag
    15061 ggtatgacca ttttcatcaa gaatggtaac ccccatcggc aacgtacccg gtaagaaaaa
    15121 gttctccatc cggatggtct gctcgacacc caaaagcgcc tgcaaccggt tcgccagata
    15181 aactggcgtc aacgcgtaaa aatacccgac gccagggcgc ggaccttcgc tgatccagta
    15241 gaggttactg ccgctatcat cttgtggtgc atttcgatat ttattgatgc gttcatgcaa
    15301 agctttcaac gcggtatcgc gttccactgg catatcacgc agaccgaaat tggccatgca
    15361 gaggttatcg ctgccgatta aaaatacccg gtttagatcg taagccgcag aaaaattatc
    15421 gcgccagtag cgcataaacc acgccaatga ctccagagaa cctcgccagg tgttactcat
    15481 tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg cctgcgtttc
    15541 tcgtccacgc ggggaaagca caccgttttc tgccgataag cgattttcgg cgatgtactt
    15601 cagctctttc atcacatcag aggtgcgttg aataaagcgc tgagcctgat cggaactcag
    15661 attaaattcc tgacgaattt ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac
    15721 ggatgaaaaa gcaatcaaca gccagagcac taacgccaat gctctgaaca tgtagcgcga
    15781 ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa ggggcgaagc tccgcctcag
    15841 gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat gttctctctt
    15901 ctctggaata tgatacaccg ccgagaaatc atcaccttaa cctctgataa tcgtcatata
    15961 ccggacaaga ctagtggatt tcagcatgca ttatatgaag tggatttatc cacgccgctt
    16021 acgcaatcaa atgatcctga tggcaatcct gatggtcatt gtcccaacgc ttactattgg
    16081 ttatatcgta gaaacggaag gacgttcagc agtcttatct gaaaaagaga aaaaactttc
    16141 tgccgtggtc aacctgctta atcaggcact aggcgatcgc tatgatctct acatcgactt
    16201 accacgtgag gagcgtatcc gcgcattaaa tgcagaactt gcccccatta ccgaaaatat
    16261 cactcacgcc ttccctggca tcggtgctgg ttattacaac aaaatgctgg atgcgataat
    16321 cacctacgcg ccttcagcgc tatatcagaa taatgtcggc gttaccattg ccgcagatca
    16381 ccctggtcgc gaagtgatgc gtacaaatac ccctttagtt tattcaggca ggcaggtgcg
    16441 cggcgatatt ttgaattcaa tgctccccat tgagcgtaat ggtgaaatcc tcggctatat
    16501 ctgggccaat gaattaaccg aagatattcg ccgccaggcc tggaaaatgg atgtgaggat
    16561 tatcattgtg ctcaccgctg gtttgctgat aagcctgctg ttgattgtcc ttttctcccg
    16621 tcgcctgagc gccaatattg atatcatcac cgatggcctc tcgactctgg cacaaaatat
    16681 tcccactcga ttaccacaat tgcccggtga aatggggcaa atcagtcaga gtgttaataa
    16741 cctcgcccag gcactgcgtg aaacgcggac acttaacgat ctgattattg aaaacgctgc
    16801 cgatggcgtc attgccattg accgccaggg tgatgtaacc accatgaacc cagcagcaga
    16861 agttatcact ggctatcaac gccatgaact ggtagggcag ccttactcca tgttgttcga
    16921 caatactcag ttctacagtc cagtactgga tacgctggaa catggcaccg aacatgtggc
    16981 gctggagatc agttttccag gtcgtgaccg caccattgaa ctcagtgtca ctaccagtcg
    17041 tattcataac acgcacggtg aaatgatagg tgctttggtg attttctctg atttaactgc
    17101 ccgcaaagaa acccagcgcc gcatggcgca agcagaacgc ctcgccacac tgggtgagct
    17161 gatggctggc gtcgcgcatg aagtacgtaa tccgttaacg gctattcgtg gttatgtaca
    17221 gatcttgcgc caacaaacca gtgacccaat acatcaggaa tatctgtccg tagtactcaa
    17281 agaaatcgat tcaattaaca aagttattca gcaattgctc gaattttcac gtccacgcca
    17341 cagtcaatgg caacaagtca gcctcaatgc attggttgaa gaaactctgg tactggtaca
    17401 aaccgccggc gtacaagcgc gggtcgactt cataagcgaa ctggataatg aattaagccc
    17461 gattaacgcc gatcgtgaac tgctcaaaca ggtactactg aatatcctga tcaatgccgt
    17521 ccaggctatc agcgcacgag ggaaaattcg cattcaaacc tggcaataca gcgactcaca
    17581 acaggccatt tcgatagagg acaacggctg tggcattgat ctctcgctgc aaaaaaagat
    17641 cttcgatccc tttttcacca ccaaagcctc aggaaccggg cttggtctgg cgttaagtca
    17701 acgcatcatt aatgcccatc agggtgatat tcgcgtcgcc agtttgccgg gctacggcgc
    17761 aaccttcacg cttattttac cgatcaaccc gcagggaaat cagactgtat gactgctatt
    17821 aatcgcatcc ttattgtgga tgatgaagat aatgttcgcc gtatgctgag caccgctttt
    17881 gcactacaag gattcgaaac acattgtgcg aacaacggac gcacagcatt acacctgttt
    17941 gccgatattc accctgatgt ggtgttgatg gatatccgca tgccagagat ggacggcatc
    18001 aaggcactaa aggagatgcg cagccatgag acccggacac ccgttattct gatgacggcc
    18061 tatgcggaag tggaaaccgc cgtcgaagcg ctacgctgcg gagccttcga ctatgttatt
    18121 aaaccgtttg atctcgatga gttgaattta atcgttcagc gcgctttaca actccagtca
    18181 atgaaaaaag agatccgtca tctgcaccag gcactgagca ccagctggca atgggggcac
    18241 attctcacca acagcccggc gatgatggac atctgcaaag acaccgccaa aattgccctt
    18301 tctcaggcca gcgtcttgat tagcggtgaa agcggcaccg ggaaagagtt gattgccaga
    18361 gcgattcact acaattcgcg gcgggcaaag gggccgttca ttaaagtcaa ctgcgcggcg
    18421 ctgccggaat cgttgctcga aagtgaactg tttggtcatg aaaaaggtgc atttactggt
    18481 gcacaaacct tgcgtcaggg attatttgaa cgagccaacg aaggtactct gctcctcgac
    18541 gaaattggcg aaatgccgct ggtactacaa gccaaattac tacgcattct acaggaacgg
    18601 gaatttgaac ggattggcgg ccatcagacc ataaaagttg atatccgcat cattgctgcc
    18661 accaaccgcg acttgcaggc aatggtaaaa gaaggcacct tccgtgaaga tctcttttat
    18721 cgccttaacg ttattcattt aatactgccg cctctgcgcg atcgccggga agatatttcc
    18781 ctgttagcta atcacttttt gcaaaaattc agtagtgaga atcagcgcga tattatcgac
    18841 atcgatccga tggcaatgtc actgcttacc gcctggtcat ggccgggaaa tattcgagag
    18901 ctttccaacg ttattgaacg cgccgtcgtg atgaattcag gcccgatcat tttttctgag
    18961 gatcttccgc cacagattcg tcagccagtc tgtaatgctg gcgaggtaaa aacagcccct
    19021 gtcggtgagc gtaatttaaa agaggaaatt aaacgcgtcg aaaaacgcat cattatggaa
    19081 gtgctggaac aacaagaagg aaaccgaacc cgcactgctt taatgctggg catcagtcgc
    19141 cgtgcattga tgtataaact ccaggaatac ggtatcgatc cggcggatgt ataacaccaa
    19201 aacttgctat gcagaaattt gcacagtgcg caattttctg catagccgct cattctcctt
    19261 ataaatcccc atccaattta tcccttcata ttcaattagt taaataacta aatccaataa
    19321 tctcattctg gcactcccct tgctattgcc tgactgtacc cacaacggtg tatgcaagag
    19381 ggataaaaaa tgaaaacaaa attgatgaca ttacaagacg ccaccggctt ctttcgtgac
    19441 ggcatgacca tcatggtggg cggatttatg gggattggca ctccatcccg cctggttgaa
    19501 gcattactgg aatctggtgt tcgcgacctg acattgatag ccaatgatac cgcgtttgtt
    19561 gataccggca tcggtccgct catcgtcaat ggtcgagtcc gcaaagtgat tgcttcacat
    19621 atcggcacca acccggaaac aggtcggcgc atgatatctg gtgagatgga cgtcgttctg
    19681 gtgccgcaag gtacgctaat cgagcaaatt cgctgtggtg gagctggact tggtggtttt
    19741 ctcaccccaa cgggtgtcgg caccgtcgta gaggaaggca aacagacact gacactcgac
    19801 ggtaaaacct ggctgctcga acgcccactg cgcgccgacc tggcgctaat tcgcgctcat
    19861 cgttgcgaca cacttggcaa cctgacctat caacttagcg cccgcaactt taaccccctg
    19921 atagcccttg cggctgatat cacgctggta gagccagatg aactggtcga aaccggcgag
    19981 ctgcaacctg accatattgt cacccctggt gccgttatcg accacatcat cgtttcacag
    20041 gagagcaaat aatggatgcg aaacaacgta ttgcgcgccg tgtggcgcaa gagcttcgtg
    20101 atggtgacat cgttaactta gggatcggtt tacccacaat ggtcgccaat tatttaccgg
    20161 agggtattca tatcactctg caatcggaaa acggcttcct cggtttaggc ccggtcacga
    20221 cagcgcatcc agatctggtg aacgctggcg ggcaaccgtg cggtgtttta cccggtgcag
    20281 ccatgtttga tagcgccatg tcatttgcgc taatccgtgg cggtcatatt gatgcctgcg
    20341 tgctcggcgg tttgcaagta gacgaagaag caaacctcgc gaactgggta gtgcctggga
    20401 aaatggtgcc cggtatgggt ggcgcgatgg atctggtgac cgggtcgcgc aaagtgatca
    20461 tcgccatgga acattgcgcc aaagatggtt cagcaaaaat tttgcgccgc tgcaccatgc
    20521 cactcactgc gcaacatgcg gtgcatatgc tggttactga actggctgtc tttcgtttta
    20581 ttgacggcaa aatgtggctc accgaaattg ccgacgggtg tgatttagcc accgtgcgtg
    20641 ccaaaacaga agctcggttt gaagtcgccg ccgatctgaa tacgcaacgg ggtgatttat
    20701 gattggtcgc atatcgcgtt ttatgacgcg ttttgtcagc cggtggcttc ccgatccact
    20761 gatctttgcc atgttgctga cattgctaac attcgtgatc gcgctttggt taacaccaca
    20821 aacgccgatc agcatggtga aaatgtgggg tgacggtttc tggaacttgc tggcgtttgg
    20881 tatgcagatg gcgcttatca tcgttaccgg tcatgccctt gccagctctg ctccggtgaa
    20941 aagtttgctg cgtactgccg cctccgccgc aaagacgccc gtacagggcg tcatgctggt
    21001 cactttcttc ggttcagtcg cttgtgtcat caactgggga tttggtttgg ttgtcggcgc
    21061 aatgtttgcc cgtgaagtcg cccggcgagt ccccggttct gattatccgt tgctcattgc
    21121 ctgcgcctac attggttttc tcacctgggg tggcggcttc tctggatcaa tgcctctgtt
    21181 ggctgcaaca ccgggcaacc cggttgagca tatcgccggg ctgatcccgg tgggcgatac
    21241 tctgttcagt ggttttaaca ttttcatcac tgtggcgttg attgtggtga tgccatttat
    21301 cacccgcatg atgatgccaa aaccgtctga cgtggtgagt atcgatccaa aactactcat
    21361 ggaagaggct gattttcaaa agcagctacc gaaagatgcc ccaccatccg agcgactgga
    21421 agaaagccgc attctgacgt tgatcatcgg cgcactcggt atcgcttacc ttgcgatgta
    21481 cttcagcgaa catggcttca acatcaccat caataccgtc aacctgatgt ttatgattgc
    21541 gggtctgctg ctacataaaa cgccaatggc ttatatgcgt gctatcagcg cggcagcacg
    21601 cagtactgcc ggtattctgg tgcaattccc cttctacgct gggatccaac tgatgatgga
    21661 gcattccggt ctgggcggac tcattaccga attcttcatc aatgttgcga acaaagacac
    21721 cttcccggta atgacctttt ttagttctgc actgattaac ttcgccgttc cgtctggcgg
    21781 cggtcactgg gttattcagg gacctttcgt gatacccgca gcccaggcgc tgggcgctga
    21841 tctcggtaaa tcggtaatgg cgatcgccta cggcgagcaa tggatgaaca tggcacaacc
    21901 attctgggcg ctgccagcac tggcaatcgc cggactcggt gtccgcgaca tcatgggcta
    21961 ctgcatcact gccctgctct tctccggtgt cattttcgtc attggtttaa cgctgttctg
    22021 acggcacccc tacaaacaga aggaatataa aatgaaaaat tgtgtcatcg tcagtgcggt
    22081 acgtactgct atcggtagtt ttaacggttc actcgcttcc accagcgcca tcgacctggg
    22141 ggcgacagta attaaagccg ccattgaacg tgcaaaaatc gattcacaac acgttgatga
    22201 agtgattatg ggtaacgtgt tacaagccgg gctggggcaa aatccggcgc gtcaggcact
    22261 gttaaaaagc gggctggcag aaacggtgtg cggattcacg gtcaataaag tatgtggttc
    22321 gggtcttaaa agtgtggcgc ttgccgccca ggccattcag gcaggtcagg cgcagagcat
    22381 tgtggcgggg ggtatggaaa atatgagttt agccccctac ttactcgatg caaaagcacg
    22441 ctctggttat cgtcttggag acggacaggt ttatgacgta atcctgcgcg atggcctgat
    22501 gtgcgccacc catggttatc atatggggat taccgccgaa aacgtggcta aagagtacgg
    22561 aattacccgt gaaatgcagg atgaactggc gctacattca cagcgtaaag cggcagccgc
    22621 aattgagtcc ggtgctttta cagccgaaat cgtcccggta aatgttgtca ctcgaaagaa
    22681 aaccttcgtc ttcagtcaag acgaattccc gaaagcgaat tcaacggctg aagcgttagg
    22741 tgcattgcgc ccggccttcg ataaagcagg aacagtcacc gctgggaacg cgtctggtat
    22801 taacgacggt gctgccgctc tggtgattat ggaagaatct gcggcgctgg cagcaggcct
    22861 tacccccctg gctcgcatta aaagttatgc cagcggtggc gtgccccccg cattgatggg
    22921 tatggggcca gtacctgcca cgcaaaaagc gttacaactg gcggggctgc aactggcgga
    22981 tattgatctc attgaggcta atgaagcatt tgctgcacag ttccttgccg ttgggaaaaa
    23041 cctgggcttt gattctgaga aagtgaatgt caacggcggg gccatcgcgc tcgggcatcc
    23101 tatcggtgcc agtggtgctc gtattctggt cacactatta catgccatgc aggcacgcga
    23161 taaaacgctg gggctggcaa cactgtgcat tggcggcggt cagggaattg cgatggtgat
    23221 tgaacggttg aattaatcaa taaaaacacc cgatagcgaa agttatcggg tgttttcttg
    23281 aacatcgacg gcgaaggtaa ccccattaat caccagtcaa aacttttcac cagcgtcagc
    23341 tcgccagcat tacgcatcgg tacaataaat gtttcctgtt tctcattgac cgatccttca
    23401 tcggtgatca gcgtcagttg ggcggtggtt aattccgttt cgctgcgccc accatagtag
    23461 ttgatataca cctgatagcg cccgtgaatt ggcgcgggca tggcgaaaat ctcgggtccg
    23521 taccccgtcg tgacatccat atccagtgca ccactgtttt tcagcacggt gttaccgtac
    23581 caggcgtgtt cgccatcggg cgtaacaacg tgaaggtcga ggtcggtatt gtccgtatcc
    23641 cacgagagaa ccagccgtaa acgtgcacga atcgttcctg tacccggcgt tgagtaaaac
    23701 tgcatttttt gtcggctttg cccatccggg ctgatgacct gcacgctgtt gctgccttca
    23761 gtgaaaatat aagggcgtgc aaatgaacca tcggattcaa ttctctgcgg catactggca
    23821 ccgttgacca ccagccggcc ctgctgaact ttacccgccg cagcataatt ctttattttg
    23881 ccgcggatct gcgctgagat attttgatca tctgccatgt tgacggacga cgccgggtaa
    23941 ttgatcgatt gtgaaaaact ggcatcttcg ccctctgccg gatgccagcc tgagagcggt
    24001 gcatcaatct cgacttcctg cacaccttca ctgtgagcga caggcgaaag cgccaccagt
    24061 aacaacggaa gaaaaatctt tcgcatagca ttagtccatt aataattgtc gggtcagcgt
    24121 ttcgatatag ttctcatcaa gcccggtggg ataaccatca aaggccaggt gcagatactc
    24181 atgggttaaa tccagccgat cctgaagcgt aaagaagtta cgaataaaca gccgtttctg
    24241 ctgccgatcg gtatagggga aacctgagac taaacggcaa accgcaaaca cgtctggttc
    24301 gttgtacccc gtctcagctt gtaatatacg ccgccactgc ggcatttttt tcgccagcca
    24361 ggctttcgct ttgggtaata actggcaggt tgagcgtggc gctccccagc ggctaagact
    24421 gttgtcggga taagcaaacg cgagtatctg atcgtaacgc tctccctgcc ccgcctgggc
    24481 cgtggcttgc cgccaggaaa gtgtcccttc ggtagcgcga ctgccatgat aatgaaccgg
    24541 atcgcctgca tagatgagat cctgcgtcca ggcggtcatc gttcgcgccc ccgtggtcgc
    24601 tggcgaagca gaaacacgct gcgtggcgct gctgtcgggg atggtcaggc aatcgccttc
    24661 ccgattggcg ttttgctgta aaaaagtgcg aatcgccacg gtcatggctt tcgctgcctc
    24721 agggggcgta cttttcgcct cgcgatccag aactctggca acgtactctt cacgatcgag
    24781 atgcgactgc aatttcagtt tgcccttttc gctcagtaag gtcgtctcac cgtggctgac
    24841 aaacgtaatg tggttaccat tagtaaacgt cacacgatag cgcccattaa gtacgccggg
    24901 atttactgcc gtcgtactct tttccgctgt gattttcttc agcggatagc gggcaaacag
    24961 ctccacctct acacattgcc cactggcaac ctgcgtcggc actggcaaaa cccgattaag
    25021 caccgtcgca taacgggtta gtaccgtttt gctggtgcca ctgccagtaa cccacagcgg
    25081 tgtaccatcg gttaaccagc cagcaaaacc accctgacgg atttcctgtt tatcatcggc
    25141 aaaccagctc caggttttca cccgtacccg actaccgagc atggatgcga caccgatttt
    25201 cgcctcgtcc agcaccacat ccagtaagac ttcctgagct ttgttttgcg cgggtaacgt
    25261 cgctaatgaa tcaagcagcg atttaaccgt taccgaggtt tctggcttca gcgtagtcag
    25321 agacgctaac cacgcaggtg cctgacgctg ttgccagtat tgcccccaca catctgcgcc
    25381 aatatgcaac cgctgcggcg caaaatagag cccacaggaa cgtaccagcg cggtatcgcg
    25441 cgttatgctc tccccagcct gacaacaata aacctcttcc ggggagtttc cgcgacactg
    25501 gtagggctgc tccggttgat gggtatcctc cagccaggcg taaacataca gtttccacaa
    25561 actccctaac ggcgtggtga gcgagtcagg taatgctgag actttggtca cgcctgatga
    25621 cgacagctga tacagttgat cgtgctgcgc accgcgcagc accagttgta acggcgtctc
    25681 ctccgcaagt gttggcagag taaccaacgc caatagccag acgattctgc gccagttcac
    25741 ttatttcacc tgcatcccgg tccattcact ccctgccgca acactttgct gcgcaggtgc
    25801 ataggaacgc acataacgag caggaggcag aacgaattgc cctttttgcg agaagcgcag
    25861 caaatggcgg aaagtgaccg ttcccgtcag ttctttcacc ggcaccatat aggccagttc
    25921 gcccatttca tttcgcgctt tttccagcaa ttgcccctgc tgtttcgcgg cgttgggttt
    25981 attgaccgaa atgccccatg ttgtgcgctc aacgtcggct cccggtggca gcggtacttc
    26041 cacctgaccg tagcgcagaa ctgcatcctg ctcgctggta agcgtgattt catcgagata
    26101 cagcgcatcg ctgtcaatct cattgctggt caccggttgc agaataaagc tcatctcttc
    26161 ttcaccaggg ataagccgat acaactggcg ttcaacggtc accggaatgt tactttgttg
    26221 agccattttt gccggctcac gccagcggac ctgcacattt tgcggcgata attcgtcacc
    26281 aaaagagaga atgtccggca cgccctgacc aacccaacgc cagtcttcgc cccctccagt
    26341 taacttatgt ttagcccatg cgcccgcagg cgcaggcaaa acaactggag gcattgtcgc
    26401 catatatttc gccagccagt tcatggccag cgcgcgctca atagtggatt gctcagcggt
    26461 caaaccactt aaaatagcgg ccgtctgggt agcgtcgcca ccgctattaa gcagcacaac
    26521 cgtgtgcgcc agcggctgat tgctgtttgc cgctttatcc cacgcatatt gctgaacctg
    26581 tggcatcacc gtggacttca actgcgcttt tttcagcaac gtcatggtta agacgtttgc
    26641 caccgcatct gccagtggag actccggtgt atcgaggatc aggctatcat tgatatcgcg
    26701 ggtgtcttcc tcagagaaat cttcagtttt agttccgcgc cgggcgatag cttcatccaa
    26761 ccctttcaac aacgttttgc acggcaaatt catctcctgc gcccatgcca gcaccagcgc
    26821 ccgatgcaat aacggcatgt tatctgcctg ctccgcgtag ctgtcgagca tatgctgcca
    26881 gtattccggt tgttgcgtta cgccgatcgc ctggctggcc tgccagtcgg cgtaccatgc
    26941 ccatgccgta aggaaggcgt caccattgcc atcttcaccc caccaggtaa agcgcgctcc
    27001 gggccccgcc agttgcatca gccgcagacg gttatcctga atcatctgac gaatgtcgtt
    27061 agcggcggca ctttgatgat cggcaagcga acgccaggcg agactgagcg ggatcagacg
    27121 gctaccggtg ttgattacgc caccccacgg ttcatcgact aacgcatcaa gattgttgcg
    27181 gaaaatctcc tgcggcgttt cactactttg tagccggata ttgctcgcct gctcgggcaa
    27241 catcagcgcg ttatcgccac caccgagcat gacatttttc tgctgttcaa cgggccagct
    27301 gttatccaca aaagacagtt ttgtgctaat gctgtcctgc acttgcccat tttgttgcag
    27361 ttctgcactt aacaagccag attgctgaat attctgcgtc agcgaaatat aattcgcccc
    27421 tttgtgcagc gtcagcgtct ggcgcatctc agcgcctgca aatttagtca ccagcgccac
    27481 cggttcgtta tcctgctgac tgaagataaa cagtcctgcc gccggtttgt cgcccacgcg
    27541 atacaccgtt ggcatactcc acttcatgta gagatttttt tccgaacgca gataagcacg
    27601 cccctgcccg accagcccgt cgccgttcat cccacgcgcg gtgatacgcc agcgggttaa
    27661 cgaatcaggc atcaggaacg tgaaatacgc tttgccttgt ttatcggttg tgagtgacgg
    27721 catccatgcc gcggtatcca cctcttcacg ccgtggacgt tcaagcattt ttactcgccg
    27781 ctcgctgcgg ttagttgcgc caggcgcaac cggctcgctg gagagcgcct ggtcgtagct
    27841 gataaacgac agactggagc tggtacgcac attgttacgc cccagcggat agaaaaattt
    27901 gccgatattc ggcgcgattt ctggttgcag cgcgtagatc atttcatcga ccacgcctac
    27961 cgttagctgc gcagaaacag gtttaccttt cagcgacgag gttaattcga cattgaccag
    28021 ttcaccaggc tggtaatggg ttttgtccgt tttcacccgg atatccagct ggggaacggc
    28081 aactttgatc ccggcgttct gaaaactgta ctgaccgtta cgggtataca gcaccgaaaa
    28141 agtgatgtta ggcgcaaagg aattgctcac tggaacccgg gcttcatact gggtatcgtt
    28201 taaacgttgt agcgttagcc agtttgccgg atgcgaaagc agcgactgct gttccacgcg
    28261 atcgcgttcc agcgtcaata atgcttcatc aattggctcc ggaaaggtaa tcagcatctt
    28321 cgcggtttcg cctggctggt acagcgtttt atccgccacg atatctaccg taccagtatg
    28381 cgccgtgctg cccttaccgc tgacggcatg acttaaccca gcgagaatta agccgtcttt
    28441 atcgcgtaat gtcagattgt agttgccagg tttagcgaaa ttgacggtaa aggatttgcc
    28501 gcctgacggt agctctccgc tatggctcgt gcggtcttcg agacgcaacc attcatacgt
    28561 aacaggaacc tgttttgaag attccagcgc ggcataacgg aacacaaccg actcgccgct
    28621 attactgtat tgtgcggcag tacttaatga gtaatgcgcc agaccgcgtt caatgaggat
    28681 ctctttggtg gtggtgacgc gatacgccgc gccgtcactg gcggagacgg ttaacaaata
    28741 gcggctcggt ttatcggcgg cggggagatt taacgccaca tgaccgctgg cgtcggacac
    28801 cgtttcgctg ccttccagcg acacggggaa acgtccggca taacgcaaat cgttaccgac
    28861 cattgataat tgctgagcgc gcaaacttaa ctgcacgcgg gcatttttta ccggctcgcc
    28921 atccgggtag agcagttgca gtttgccgct gaccgcttcg ccagttttga actctttttt
    28981 ggcgagagct aaaccaatct cgaaatgtgg cttgatgtag tttgccacgc gaaaactgct
    29041 gctatagacc tgattgcggt aagcaagacg taactcataa cctccggcta cggcattttc
    29101 tggcaggcgg aaacttccct gcccgccatt gcgcgcatcc agcgtgacat tgacggtttg
    29161 caacagactg ccgttggcgt ccagcaccga aagcttcgcc ggggcgctga cgatggggga
    29221 tgaatgcaac ggatcgtgga actcgcggcc gatcacttta acatcgacac gatcgcctgc
    29281 gcgatatagc gggcgatcgg taaaaatata caagcgggtg ttgtagattt cgctttcgta
    29341 gaagaagttc tcggagacaa aaacgccgcc ttcagcatcc ttacccagaa tgtatgaacg
    29401 ttctggcgat atatgttgta actgcaaggt accgctgtca tcggtcacac cgcgggtcat
    29461 cacgccaaga ccgtcagtcc acaagatctc tgagccgggc ttcgcttcac cctgtttttt
    29521 acccgcggtc cacaccagaa gctctttgcc tgacactttg ctaagcgcca cggtatcgga
    29581 aacaaacacc accgtcgtcg cccgataccc accaaccatc gcctcgacga ggtacagtcc
    29641 cggctcttgt tggccgagag gaatataaat gttacccggc tgcggcgaga tgaaattgct
    29701 ggatgcgcct tccagtttta ccccttgctg cggctcgaac ggtttagcct gccatagtgg
    29761 ataacggaac tgtttcacca ggggatattt tttcagcggg gaaaactggt tgttctgtac
    29821 ataacgggaa ggtttaataa tggcattgcc gagctgtaat tcgggtaacg cctgagtcac
    29881 attctgccgt gactgagaag agaaagtacg ctgcatcacg cggcgagatt tgccgtacca
    29941 gttatcccac agccaggtta gcgtattgtt cagcccgtcg cccagatatt gcggttgcac
    30001 cacaatgcga tgcaggtttt tctgctggcg caaaaatgcc atcgggtcag gaatacgata
    30061 caggcgaacg tccacgccgc cgtactcttc catctgatag cgccgataat cacgccccgg
    30121 cgcttccagt cgcactttcg cctcttcact gctgctaaaa ctgctgtcag caagcaaaaa
    30181 gaatgttccc ccggcaggcg gcgcatagtt gctggaagga agcgaatcat cagcattagc
    30241 aagccctgtt cccacaagag acagggacaa gcacgctgct atcgcgccag aaaatttaaa
    30301 cgataaatgc caatgaaatt gggattggaa tcgttgggta tccatcgggt gtccttccat
    30361 gtcataagtt gttgcagact gactgcgcgc attccgttgt cagttttcgt ggcgcttccg
    30421 gtgtggtaga tgacgtaacg ccccatccag accattaagt gctgggcatc gccctgatcg
    30481 aaaaaaatca tatcgccagg cagcgcctgg tttatgtcct ggccaataaa ctggctgttg
    30541 tactgaatca aattaatcgc ggtcacatag gggccggttt tcccgttccc ctgattccag
    30601 ttttgcgcca gttgacgctg ttcaggtgtt agcgtcatct ctggcggcaa atactggcta
    30661 gataaaccgt tactttttag ccatttactg tcgtgaactt tcagcgtctc gttcgccgca
    30721 aatcgcacca ggcccgcaca atcctgctga taccagcgtg gacttggccc ctggcggagc
    30781 tgttcttgtg caatgcgtac aaaccaggcg cgaaacagcc cggattgttc gacattcagc
    30841 atttcgctat gggcaacaac acagccagca aatcagcgcc agcagcccgt gcctcatagc
    30901 ggctgccagg ttatgggtag ccactgccag gcggcaccgg gttccatctg ggccagcttc
    30961 atgacataac gcggttgttg agataaagcg tccagcttcg gcattaataa agtttgtgcg
    31021 gcgttataaa aaaccggttc gagattcttc ggcagactgg tcaacgtttc gttacgcagc
    31081 agtttcgcta ttccttgtgg attgatatag agcggaacga tgccatcagt gggtattaca
    31141 tccaccattg ccgggcgggt tttattcagt gtttgcagcg cgttattaac cagcgtgtca
    31201 tcgagggaga aaagcagcgt tttgttttgc atcgccagcg acacacggaa aaaataatcc
    31261 gacattaatt gatcgggttg cgccgcctgc gctttcggat actggccgta tcgggaactc
    31321 acttcgcgac gccagatttg cgcttcgccc tgctgagtct ggcttaccgg caaaacacct
    31381 tctggcgctt tgctttcgtg cgcaccaata ttttgcgtaa acagtttccc tggcaattgc
    31441 gcctgttcgg cagtgccatc aaactgaccg acaaacagcg gggtttgcaa ttttgagtct
    31501 tcataccagc acagccccgc ggcaccgtct aacgccccat tcagcttgtc gttttcctgg
    31561 ctgatgtgcg aaagcatctc ttcggcaata ccgtgtgaat acggcaccgc cacacagaag
    31621 ctggctccgg caggcatact gttccatacc ggcgtaaaat cgaaactggc atctacgctg
    31681 gcggattcat catttaacgc cacaaaactg tgccagccgt cgttacccat ttcgaagcgt
    31741 acgccagcaa aagaaggcat taaccgctgg tagccaaacc ccagccacct ggcgctgact
    31801 acgatgcgct ggcgtactgg cgttttttcg gcagtacgct cttccaggcc aaagcttgct
    31861 tgccagcgtt ttttgccgct caacaaatca cctgtgatcg ccgtggcttc ggtatcctgc
    31921 tgatcatctt taaacaacat atccgtgctg gaaaacacca gcatcttgtc ctgataagtc
    31981 gcgaacatca gggcgttatt gccgttatag cgcaactgat aaacaggaac agtttcacta
    32041 tttatcttga tgctactgat ttccgttttg cttaactggc tgtcgctggt agcggcaaac
    32101 aacaatggct ccagcagttt gcttaaacca ctgcgctgga tcagcaccat ataatgtgaa
    32161 agatggcctt gtttatcgtg ccacagcgct gcctgcgcgg gctgatctaa gagtgacgaa
    32221 aagagcttat ctttcagcgt gagatcgtgt tcatagacaa tacgacgaat gctgccttca
    32281 atgcccagac gatcggcatg attctgataa tagaaaacga aatcttcgct cagaacatcg
    32341 tggagaaacg gaatggtgag gagatctttg ggaagctggc tcagagagtc gctgtcgaga
    32401 aagaggtccg gctcattgag atcgatttgc agattgttgt gcaccaccag cggcgacaac
    32461 gttttttctg gcccactgcc agcatattgc aacgcccaga cgccagcgga aagcagtgct
    32521 attgcgccaa aacctacaag accatagaac cgccagcctt tcgccttttt ttcaccactc
    32581 attgccacat tccttgtgta tagccagcca ttttttacgg gcacagccaa actttaccgt
    32641 gccctaatac gacaaaagcc cagactttgc agcctggact tttcaattca aacaagggag
    32701 atagctccct tttggcatga agaagtaaaa ttattcttct tctggctcgt cgtcaacgtc
    32761 cacttccgga gcgatttcat cgtccccttc cgcggcactg ccgtcgatgg tatccagatc
    32821 ttcctcgtca accggttcag caacacgttg cagacccact acgttttcat cttccgcagt
    32881 acggatgagg atcacgccct gggtgttacg gcccacgatg ctgatttccg aaacgcgagt
    32941 acgtaccagc gtaccggcat cggtgatcat catgatctgg tcgcagtcat ctacctgtac
    33001 cgcgccaaca actaaaccgt tacgttcggt aaccttgatg gagataaccc ctttcgtcgc
    33061 acgcgacttg gttgggtatt ccgccactgc ggtacgttta ccgtaaccgt tttgcgttgc
    33121 ggtgaggatt gcgccatcgc cacgaggcac gatcagagag acgactttat cgccttcacc
    33181 taagcgaata ccgcgaacac cggtggtgtt gcagcccatc gcacggacag aagactcttt
    33241 aaagcgcacc actttacctt cagcggagaa cagcattact tcgtcttcgc cgctggtcag
    33301 gtcaacgccg atcagctcat cgccgtcaac cagtttgatc gccactttac cggcggtacg
    33361 cagacggttg aactcggtga ggacagtttt cttcacggta ccgttagcgg tcgccatgaa
    33421 gactttcacg ccttcttcaa actcggtcac tggcaggatc gcagtgatac gttcgtcctg
    33481 ctccagcggc agcaggttga cgatcggacg accgcgcgcg ccacgagtgg cttccggcaa
    33541 ctgataaact ttcatcgaat agacgcgacc acggctggag aagcacagaa tatggtcgtg
    33601 agtgttcgcc accagcagtc ggtcgataaa gtcttcttct ttaatacgtg cggcagattt
    33661 acctttcccg ccacgacgct gcgcttcgta ttcagaaagc ggctgatact taacgtagcc
    33721 ctggtgagag agcgtcacga ccacatcttc ctgggtgatc agatcttcca ggttgatgtc
    33781 tgcgctgttg gcggtgattt cagtacgacg tttgtcaccg aactgttcac gaaccagctc
    33841 cagctcttca cggatcactt ccatcagacg atcggcgcta ccaagaatac gcaacagttc
    33901 cgcgatctga tccagcagct ctttgtattc gtcgagcagt ttttcgtgct caagaccggt
    33961 cagtttctgc aaacgcagat ccagaatcgc ctgagcttgc tgttcggtca ggtagtacag
    34021 accatcacgc acgccgaact ctggctccag ccattccgga cgcgcagcat cgtcgccagc
    34081 acgttcgagc atcgcggcaa cgttgcccag ctgccacgga ttagcaacca gcgcagtttt
    34141 cgcttctgca ggcgtcggcg catgacggat cagttcgatg atcgggtcga tgttcgccag
    34201 cgccacggct aatgcttcaa ggatatgagc acgatcgcga gctttacgca gttcgaaaat
    34261 agtacgacgg gtcaccactt cacggcggtg acgaacaaac gccgcgatga tgtctttcag
    34321 gttcatgatc ttcggctgac catggtgcaa tgccaccatg ttgataccga aagaaacctg
    34381 caactgggtc tgggagtaga ggttgttgag cacaacttca ccgaccgcat cgcgtttcac
    34441 ttcaatcacg atgcgcatac cgtctttgtc agactcgtca cgcagcgcgc tgatgccttc
    34501 cacgcgtttt tcttttacca gttccgcaat cttctcgatc aggcgcgctt tgtttacctg
    34561 atacggaatt tcgtggacga taatggtttc acgaccggtt ttggcgtcaa cttccacttc
    34621 tgcgcgagcg cggatataca ccttgccgcg accggtacgg taagcttctt caataccgcg
    34681 acgaccgtta atgattgccg ccgtcgggaa gtccggcccc gggatgtgtt ccatcagccc
    34741 ttcaatgctg atgtcttcat catcaatata cgccagacaa ccgttgatga cttccgtcag
    34801 gttgtgcggc gggatgttgg ttgccatacc tacggcgata ccggaagaac cgttcaccag
    34861 caggttagga attttggttg gcatgacgtc cggaattttt tccgtgccgt catagttatc
    34921 aacgaaatcg accgtctctt tttcgagatc ggccatcagt tcatgggcaa ttttcgccag
    34981 acggatttcc gtataacgca ttgccgccgc agagtcgccg tcgatagaac cgaagttacc
    35041 ctgaccgtct accagcatat aacgcagcga gaatggctgc gccatgcgga cgatcgtgtc
    35101 atagaccgcc gagtcaccat ggggatggta tttaccgatt acgtcaccaa cgacacgggc
    35161 agatttttta taggctttgt tccagtcatt gcctagtacg ttcatggcgt aaagtacgcg
    35221 acggtgtacc ggcttcaggc catctcggac atctggcagc gcacggccaa caatgaccga
    35281 catcgcataa tccagatagg agctcttcag ctcttcctca atgttgaccg gtgtaatttc
    35341 tctcgcaagg tcgctcatct aaccgctatc cctctactgt atcccggatt caaaggtcgc
    35401 aaattataac acagccgcgc agtttgaggt aaacctatac gctttattca catccaatgc
    35461 ctgatatact cgtttgtctt gccaattacg gagtagaagt gccaatgaat gccgaaaaat
    35521 cgccggtaaa ccataacgta gaccacgaag agatcgctaa atttgaagcc gtcgcctccc
    35581 gctggtggga tctggaaggt gagttcaaac cgctgcaccg cattaacccg ctgcgtctgg
    35641 gctatattgc cgagcgtgct ggcggtttat ttggcaaaaa ggtgctcgat gtcggttgtg
    35701 gcggcggcat tctggccgag agtatggcgc gcgaaggcgc gacggtgacc ggtctggata
    35761 tgggctttga gccattgcag gtggcaaaac tgcacgcact ggaaagcggc attcaggtgg
    35821 attacgtgca ggaaaccgtg gaagagcacg cggcaaaaca tgccgggcag tatgatgtgg
    35881 tgacctgcat ggagatgctg gagcacgtcc ccgatccgca gtcagtggtc agagcctgtg
    35941 cgcaactggt gaaaccaggc ggcgatgtct ttttctcgac acttaaccgc aacggcaagt
    36001 catggctgat ggcggtggtt ggtgcggaat atattttgcg catggtgccc aaaggcacgc
    36061 atgatgtgaa gaagtttatt aaaccggcag aattgctggg ctgggtggat cagaccagtt
    36121 taaaagagcg gcatatcact gggctgcatt acaacccgat cactaatact tttaaactcg
    36181 gccccggcgt ggatgtgaac tatatgctgc acacgcagaa taagtgaggt tgatgtttgg
    36241 ccgcgccaat gcctgatgcg acgcttgccg cgtcttatca ggcctacaaa tgctccccgt
    36301 aggccggata aggcgtttac gccgcatccg gcaaccgtgc cgactagaca gtgattacca
    36361 tttcaccgtc atcgacaaaa accctgccgt ctgggcaaaa tcatcactcc ctttctgcca
    36421 cgccacgctg ccgcgcaggg acactcgctg actgatattg cccgtgactc ccacttttat
    36481 ttcaccccgt tgcttcaccg catcgtcact gatagtgctg ccatcctctt caatttccgt
    36541 cgaatgggga tcgtgataat aattcagatc taatgttggt atgacatgaa cagcggtacg
    36601 ccattcgctg tgtaaaccca gccgcgtctg aatatcatca ccctgcgatt gtgacacgcg
    36661 cgcacggtta gcggcggtaa aatcatcctg ctgcacgccc tgataaatca cctgcgcctg
    36721 cggttcaatc accacaccac gccccggtaa ccactgatac cccgcctcca gcgaggcgat
    36781 aatccccgac gagtggtaat gatctgtgcc atcttcttgt tcggaaacat cattgctaaa
    36841 ccacgcgtat tgcagccagc tatccagcca ggccccttgc ttctgattac cgtgctgaaa
    36901 ccagcttgat gtcagcccaa cggcataacc gtggttctgg ttatcggcgc gagttccggt
    36961 catattcgag cggctgtcgc cctggttatc gctgtagcca ccaacaatcc caagcatcca
    37021 ctcgccatcc gtgccccagc gcccgctaaa cagatcgccg ctaagttgca ccgtagaagt
    37081 gtcttcatgt tgagccagtt gccccgctgc tgtgtaatga taatctccgc cgataacacg
    37141 taaattcagc gtctgaccat cgccacctgc gtgatcgcgt cgctccatca taaacgcctg
    37201 atttgccgcc cgcaggttat tgagataacc gccaactttg gcattcaaca ccggctggta
    37261 agcaggcgta ggttccgggt cgggtgttgg atcaggatcc ggcgtgggat caggatcggg
    37321 agtcgggtct ggatcaggtg gcgatggcgg cgttacttct tgcgatcgca gataccagtc
    37381 gttgttatct tccaccagcg tgtagtcata cgctcccata ttgacgtagc cgctgcctgc
    37441 cagactgaac tgcgcattgt tttgaaactg cgtgggatca gctgcgaaat caaccacttt
    37501 aatgcctgtc gatgtcggct caccaatccc tgtaatggag ttaaccacca cagttgcgtt
    37561 gccagcagta ttaccgttca tcaccaattg atcgcttacc gagtcatcgc cgtttaattc
    37621 gctatcgagc agtagcgtac cgccgccggt ataatcaccg tttaccgtca gcgtatcgcc
    37681 agcgacgcca ttttgcaggc taaccgcacc ggacgtattg gtcagattgc cattaaccgt
    37741 cacgttggaa agataagtca ggctgtcagt ttcaaggttg gtattaagct cacctgtcaa
    37801 cgtcacacca tcggtggcat tcaccagcgt cccatcgccg ttaaatagtg aggcattaag
    37861 ggcgacggaa gtatcctgcc cggtcaggcg caaaaccgta ccatcgctga tgtcgatagt
    37921 gccgctggaa atagcatcaa ttgactgaat atcctgatcc gcgccagtaa cgaacgttgc
    37981 cccatcacca acccacagtg acgaagcata aggaaggata tcagcaacat cacctttcag
    38041 ggtgccttct tcgacacgta ccgccgactg cgttgtaccg ctggcggtca gctccagtgt
    38101 accagccccc gttttagtca atgtgctacc ctcatcctga tgctgcccgc tgctgtcagc
    38161 catcagtgcg ccccactgcg tgtctacccc cgcatcaact gccacttcac cgtcggcgcg
    38221 catttcaata tcacgtccgt ggcctgccgc atctgaacgc gtgtcaacgt cggaggtaat
    38281 ctccatcacg ctttcttgtt gcttatcggt aaagataacc tggcggttat agtgggtatc
    38341 tccaagctgc gagttgtcgc tcaccatcag cgtaccggag gcgatttgtg ttgtgcccaa
    38401 ataactatta ttgtttgcca gacttacctg cccgtcgctc gcatcgacca ccacgtagac
    38461 gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca cctgcaagat ctaccacatc
    38521 ctgactgccg ataaccgttc cgctgctgcc actgacttca aggccatcgt tgtatgatgt
    38581 gccgctctgc caggtggaga aatcagagag atcgagcaca ccgccgttta acacgatcga
    38641 ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg ccttccagcg aaagcaccgc
    38701 accatcatca acgactatat cgccggttag cgccatcgac tgcgcccctg ccagcacgta
    38761 gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg atgatgccag caaaactgcc
    38821 ctgattaaca gtaacgttgc caccagcatc gatatttaaa gtgccattct gaaagcccgt
    38881 caatgcgtgc acaaaagttt gttgggtcga gccaacgttt agctcagcct gattctgata
    38941 ctgatcaata ctccctatcg tcagaccgta gcagtcttgc ggatcgtcct ggcaatgcgt
    39001 atcgccgaca ttcatcaggg agttgctgcg gcccagggta acttcaccgt tttcaatctg
    39061 catctcacca gtaaagtcat tgttatctgc attaagtacc agatcgccgg aacctgtttt
    39121 ggtgattaac ccggtaccag caatagagtc aacagctccg tcattctctg tattgccaat
    39181 aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca cttaagccga gatacataaa
    39241 gccacccgcc gcagaggaag gaccatctcc atagcctgct gcgctattgt tctcatcgac
    39301 taacacgcct ccgttctggc tgtagctgtc atcaacagaa atatcaataa gatagggagc
    39361 cgtcacgcta ttggtatata tcgccccgcc ataaccttcg gcagtgttat ttgtaaaggc
    39421 agtgttattt actatcgtat aacctgaagg atgcttgctg tcgctattat tatcggtaac
    39481 atcgattgcc ccgccatcgc catcactgta acttgttgat gtatatgcct ggttgttatc
    39541 aaaaataaca tcacttaaat aaacgtcatt attaatggta taaattgcgc cacctttgcc
    39601 atcattagcg atgttattgc gaaacatggc gttagtgaca cgtaaatcga cggcaccagt
    39661 atcgttagta ccagaagaat agattgcgcc accatagccg cctgcgacgt taccggaaaa
    39721 aataacatca gtaagattca gcgttgagtt ttctttagca aatatggccc cgccgttatt
    39781 atattctcct gtaacggtgt tattggcaaa cagagtcatt ccagtttcat tttctggtaa
    39841 tagtgaaaat tccgctcctt gttgcaaaaa tacggcccca ccgctggcgt tattggtcat
    39901 atccgaaaaa accagccatt gcccatcagc gatactccag tcctgcgtaa tgcctgaaag
    39961 gctttgcctg ctggcctgac aactcgcttt gacatcatat ccctggcatg aatcagtgac
    40021 cgccgcgaca ccgttagcag agaaaagaga tgcaatcatt gacgggagta aagataaata
    40081 ctccttgcgt agaaagataa tccgcatatt aatctaacca tcattttcta taagacggcg
    40141 tatttaatcg cattatacga tatggaatat ctttcttttg taaattgttc aacccggtgc
    40201 aatggctatt ttgttattat ttgatgaata atatcagtgc gtcataattc aagttaataa
    40261 ccttcaggga tatcagttat atttaaacta aattaaagtc atgaataatt ttcttataat
    40321 ataaggtaaa ttaacaaaat ggcttagcat ttaacaataa ccgaatagaa aacaaccatt
    40381 tcgccatcaa caatctctta cattcgctta tatattgacc acaactgata catcagatta
    40441 tgtgatgact cgtgcttaga tcaatttttg caatcattag caaaaagatt aataaaccat
    40501 ctatatcaat ttatctaacc tattatgccg ttcaagaaat cgccgaacag ttatttttaa
    40561 caaatttttc tcttcccatt gactttcccg gacaccttgt ctgacctaag gtgcgcgaaa
    40621 gccacttttt ccttcctgag ttatccacaa agttatgcac ttgcaagagg gtcattttca
    40681 cactatcttg cagtgaatcc caaacatacc ccctatatat agtgttctaa gcagcttccc
    40741 gtactacagg tagtctgcat gaaactattg cggaaagaat tccaaaaaca ggtacgacat
    40801 acatgaatca gaatctgctg gtgacaaagc gcgacggtag cacagagcgc atcaatctcg
    40861 acaaaatcca tcgcgttctg gattgggcgg cagaaggact gcataacgtt tcgatttccc
    40921 aggtcgagct gcgctcccac attcagtttt atgacggtat caagacctct gacatccacg
    40981 aaaccattat caaggctgcc gcagacctga tctcccgtga tgcgccggat tatcagtatc
    41041 tcgccgcgcg cctggcgatc ttccacctgc gtaaaaaagc ctacggccag tttgagccgc
    41101 ctgcgctgta cgaccacgtg gtgaaaatgg tcgagatggg caaatacgat aatcatctgc
    41161 tggaagacta cacggaagaa gagttcaagc agatggacac ctttatcgat cacgaccgtg
    41221 atatgacctt ctcttatgct gccgttaagc agctggaagg caaatatctg gtacagaacc
    41281 gcgtgaccgg cgaaatctat gagagcgccc agttccttta tattctagtt gccgcgtgct
    41341 tgttctcgaa ctacccgcgt gaaacgcgcc tgcaatatgt gaagcgtttt tacgacgcgg
    41401 tttccacatt taaaatttcg ctgccgacgc caatcatgtc cggcgtgcgt accccgactc
    41461 gtcagttcag ctcctgcgta ctgatcgagt gcggtgacag cctggattcc atcaacgcca
    41521 cctccagcgc gattgttaaa tacgtttccc agcgtgccgg gatcggcatc aacgccgggc
    41581 gtattcgtgc gctgggtagc ccgattcgcg gtggtgaagc gttccatacc ggctgcattc
    41641 cgttctacaa acatttccag acagcggtga aatcctgctc tcagggcggt gtgcgcggcg
    41701 gtgcggcaac gctgttctac ccgatgtggc atctggaagt ggaaagcctg ctggtgttga
    41761 aaaacaaccg tggtgtggaa ggcaaccgcg tgcgtcatat ggactacggg gtacaaatca
    41821 acaaactgat gtatacccgt ctgctgaaag gtgaagatat caccctgttc agcccgtccg
    41881 acgtaccggg gctgtacgac gcgttcttcg ccgatcagga agagtttgaa cgtctgtata
    41941 ccaaatatga gaaagacgac agcatccgca agcagcgtgt gaaagccgtt gagctgttct
    42001 cgctgatgat gcaggaacgt gcgtctaccg gtcgtatcta tattcagaac gttgaccact
    42061 gcaataccca tagcccgttt gatccggcca tcgcgccagt gcgtcagtct aacctgtgcc
    42121 tggagatagc cctgccgacc aaaccgctga acgacgtcaa cgacgagaac ggtgaaatcg
    42181 cgctgtgtac gctgtctgct ttcaacctgg gcgcaattaa taacctggat gaactggaag
    42241 agctggcaat tctggcggtt cgtgcacttg acgcgctgct ggattatcag gattacccga
    42301 tcccggccgc caaacgtgga gcgatgggtc gtcgtacgct gggtattggt gtgatcaact
    42361 tcgcttacta cctggcgaag cacggtaaac gctactccga cggcagcgcc aacaacctga
    42421 cgcataaaac cttcgaagcc attcagtatt acctgctgaa agcctctaat gagctggcga
    42481 aagagcaagg cgcgtgcccg tggtttaacg aaaccactta cgcgaaaggg atcctgccga
    42541 tcgataccta taagaaagat ctggatacca tcgctaatga gccgctgcat tacgactggg
    42601 aagctctgcg tgagtcaatc aaaacgcacg gtctgcgtaa ctccacgctt tctgctctga
    42661 tgccgtccga gacttcttcg cagatctcta acgccactaa cggtattgaa ccgccgcgcg
    42721 gttacgtcag catcaaagcg tcgaaagacg gtattttgcg ccaggtggtg ccggactacg
    42781 agcacctgca cgacgcctat gagctgctgt gggaaatgcc gggtaacgat ggttatctgc
    42841 aactggtggg tatcatgcag aaatttatcg atcagtcgat ctctgccaac accaactacg
    42901 atccgtcacg cttcccgtca ggaaaagtgc cgatgcagca gttgctgaaa gacctgctca
    42961 ccgcctacaa attcggggtc aaaacactgt attatcagaa cacccgtgac ggcgctgaag
    43021 acgcacaaga cgatctggtg ccgtcaatcc aggacgatgg ctgcgaaagc ggcgcatgta
    43081 agatctgata ttgagatgcc ggatgcggcg taaacgcctt atccggccta cggctcggtt
    43141 tgtaggcctg ataagacgcg ccagcgtcgc atcaggctcc gggtgccgga tgcagcgtga
    43201 acgccttatc cggcctacgg ctcggatttg taggcctgat aagacgcgcc agcgtcgcat
    43261 caggcacagg atgcggcgta aaatgcctta tccggcatta aactcccaac aggacacact
    43321 catggcatat accacctttt cacagacgaa aaatgatcag ctcaaagaac cgatgttctt
    43381 tggtcagccg gtcaacgtgg ctcgctacga tcagcaaaaa tatgacatct tcgaaaagct
    43441 gatcgaaaag cagctctctt tcttctggcg tccggaagaa gttgacgtct cccgcgaccg
    43501 tatagattac caggcgctgc cggagcacga aaaacacatc tttatcagca acctgaaata
    43561 tcagacgctg ctggattcca ttcagggtcg tagcccgaac gtggcgctat tgccgcttat
    43621 ttctattccg gaactggaaa cctgggtcga aacctgggcg ttctcagaaa cgattcattc
    43681 ccgttcctat actcatatca ttcgtaatat cgttaacgat ccgtctgttg tgtttgacga
    43741 tatcgtcacc aacgagcaga tccagaaacg tgcggaaggg atctccagct attacgatga
    43801 gctgatcgaa atgaccagct actggcatct gctgggcgaa ggtacccaca ccgttaacgg
    43861 taaaactgtg accgttagcc tgcgcgagct gaagaaaaaa ctgtatctct gcctgatgag
    43921 cgttaacgcg ctggaagcga ttcgtttcta cgtcagcttt gcttgttcct tcgcatttgc
    43981 agaacgcgaa ttgatggaag gcaacgccaa aattattcgc ctgattgccc gcgacgaagc
    44041 cctgcacctg accggcaccc agcatatgct gaatctgctg cgcagcggcg cggacgatcc
    44101 tgagatggcg gaaattgccg aagagtgtaa gcaggagtgc tatgacctgt ttgttcaggc
    44161 agctcaacag gagaaagact gggcggatta tctgttccgc gacggttcga tgattggtct
    44221 gaataaagac attctctgcc agtacgttga atacatcacc aatatccgta tgcaggcagt
    44281 cggtttggat ctgccgttcc agacgcgctc caacccgatc ccgtggatca acacttggct
    44341 ggtgtctgat aacgtgcagg ttgctccgca ggaagtggaa gtcagttctt atctggtcgg
    44401 gcagattgac tcggaagtgg acaccgacga tttgagtaac ttccagctct gatggcccgc
    44461 gttaccctgc gcatcactgg cacacaactg ctgtgccagg atgaacaccc ttcccttctg
    44521 gcggcgctgg aatcccacaa tgtggcggtt gagtaccagt gtcgcgaagg ttactgcggc
    44581 tcctgtcgca cacgcctggt tgcaggtcaa gttgactgga ttgccgaacc gttagccttt
    44641 attcagccgg gggaaatttt gccctgttgt tgccgggcaa aaggcgatat tgaaatcgag
    44701 atgtgaattg gttgtagtgc cagatacaac gcttatgcgt cttatctggc ctacaacgat
    44761 tacatggcgt agtaatacgc tttcacctgc tcccagtccg ctttggggat tggctccaga
    44821 tatttttcca gctggcggaa gtcatgatta atcgctttat cgcgacgcaa gcggcgacga
    44881 cttttctcca ggtcaagaaa accagcttct gcattacctt ctgttttcac atagatatgg
    44941 cgaacataac aacagccatg ctgacgatta atgctatgca ttttcttaaa cgccagcgcc
    45001 actgctttca acatggcttg ccgtacttcg tcagaataag gcgatactgc atgctgggca
    45061 taccagtcag caatgctgat gaaccccgcc atatcttcag tcaccagcaa cgctcgccat
    45121 tcaccctcaa ttttcaccgc ttcaccaaaa acgatcttcg gcacgatgac accggcccgt
    45181 tccagttctt taattaccgc aacctcacgg acaatcgttg gtcggccgaa cggataacgt
    45241 acggaatgaa acagatgatg cgtcatgcgc tttacataca gctttttgcc gttgcgctcg
    45301 acgcattgca ccccgctcat accattacgg cgatagttag gctcttcaac ccagtcgccc
    45361 tctgttgccc accagtgatt aaattcgtcg tactttgctg aaactgccat accctatcgc
    45421 ctgtcatttt tattaacgac aatgactata ggtggttacc tgaggaaaat cttaatgaaa
    45481 cgtgtcgtat taatgaattt cttttgccac tactagcttg acaccgcttt tacccttcat
    45541 ttgcagcgca tagattccac cagccactac cactaatagt gcgcccagca gataaaatgt
    45601 gaatttaatc aggcggatac agcgatgaat tttattcgac agggattagg catcgcctta
    45661 caaccagagt taacgctgaa aagcattgca ggtgaattgt gttccgttcc tctcgaacca
    45721 actttctatc gacagatttc gttgctggct aaagaaaagc cggtagaagg cagtccactg
    45781 tttttactac aaatgtgcat ggaacaatta gtggcgattg gaaaaatttg atatgagaca
    45841 ggatggcgca tgaacgccat cctgcagtaa cattactctt tattaagaaa ttttactgcc
    45901 ttatcaggga aatcagtaaa cagcccattt acacccgctt tgttatacag agcatcatat
    45961 aactgattca catcaggagt gtattcaggc agtttatctg accgcacggt ataaggatgc
    46021 actaccagtt tattctgctg agcatcttgc accatgccag tgagtttgat attacccggc
    46081 tgcgatgtct cctcaatcaa catatggtaa tccggaccaa taccatctgc atattccgcc
    46141 acctgtttca tggcacccgg cttaaacatc cagtcgtagt tgtaattaac ccagcttcca
    46201 tccggctgtt tctgctgcgt ttcattccag tcggtatagg caatcagctg taccagattg
    46261 agctccatgc ccattttggg ttccagctca ttcttaatac gcttcagctc atcagcatca
    46321 aaacattgca aataaacttt atcgtcttta ccggtgtaac catatttctt cagcacttcc
    46381 agcgtttttg ccgcaatatc cttcccttcc tgatgatgga accacggcgc tttgatttct
    46441 ggataaatac cgatattttt cccggtagag tgatttaacc cctgaacaaa ttcaatctct
    46501 tcttcaaagg tgtgcacccg gaagtcggac ttacccattg ggaaacgccc cggataagtc
    46561 tgcacttttt taccgttttc aatatcgaaa ccttcggtaa atttcaacga cttaatttca
    46621 tccagcgtga aatctatcgc gtagtaacga ccgtctttgc gcgcccgatc cgggaaacga
    46681 tcggcaacat cagtaacacg atcgaggtaa tggtcatgca gaacaaccag attgtcgtct
    46741 ttggtcatca ccaaatcctg ttccagataa tccgctccct gcgcatacgc catcgctttt
    46801 gctggcagcg tatgctccgg caaatatcca ctggcaccgc gatgggcgat gactattttt
    46861 tcgttgctgt ccgccgccat tgcactgctt cccatgacta tagtgctcat catgatcgcc
    46921 atgctaaggt ttttcagcgt caatttcatg ccattagcct ccgttgcgtt cttgcagtaa
    46981 ttgttcatgg cgacgttttt cgccaatcat cacaacaatc aacaagataa ccgccagaat
    47041 gctgccgcca atcattacca taaagccgcc atcccagccg aagaagtcca cggtgtagcc
    47101 aacaatcgcg ctcgccgcca ccgaaccgcc caggtaacca aacagcccgg taaagcccgc
    47161 tgccgtacct gccgcttttt tcggtgccag ttccagcgca tgcagaccga tcagcatcac
    47221 aggaccgtag atcaggaagc cgataacaat catacaaatc atatcgacgg ttgggttacc
    47281 tgccgggttc atccagtaaa cgatagtcgc gatggtcacc agtgtcataa agaaaacgcc
    47341 ggttgcccca cggttgccac ggaagacttt atccgacatc cagccgcaca gcagagtgcc
    47401 cggaatacct gcatattcat aaaggaagta ggcccaggag gatttatcta gcgcgaaatg
    47461 cttaacctct ttcagataag tcggtgacca gtcgaggatg ccgtaacgca gcagataaac
    47521 gaacacgttg gcgatggcga tataccacag cagtttgttc ggcagtacgt actgcatgaa
    47581 gatttgcttc gccgtcagct cctgttccgc tttttcgtta tagtcgtccg gataatcatt
    47641 tttgtactct tcgatcggcg gcaagccaca ggattgcggg gtatcgcgca tcatcgcaaa
    47701 ggcgaataat gccaccagaa tggcgcagaa agcaggcata tagagcgccg catgccagtc
    47761 attgaaccag gccatcccca gcaggaacag cagcggcgga ataccaccac cgacgttgtg
    47821 cgcacagttc cacactgaca caatgccgcc acgttctttc tgcgaccacc agtgcaccat
    47881 agtacgacca cacggcggcc accccatccc ctggaaccaa ccgcagagga acaacagtac
    47941 aaacatcacc gcaatgctcg acgtcgccca tggcacaaag cccataaaca acatcactgc
    48001 cgccgccaga atcaaacctg cgggcaggaa aacgcgcgga ttcgagcgat ccgataccga
    48061 acccatgatg aatttcgaaa atccataagc aatcgagatc cccgaaaggg caaaacctaa
    48121 atcaccgcgt gagaatccct gctcaaccag ataaggcata gcaagcgcaa agttcttacg
    48181 aaccaaatag taagccgcat agccaaagaa tatccccagg aaaatttgcc agcgcaatcg
    48241 acgataagtc ggatcgatct ccgcggcagg taagcgcgct ttgtgtggcg ctggtttaaa
    48301 aatactcaac attgatagcc tccgtggccc gtggtcttat ttatgattaa cagcctgatt
    48361 cagtgagaga acctgccgtt tcttgagttg ccgcgatgtt aagaaaacat tcataaatta
    48421 aatgtgaatt gccgcacaca ttattaaata agatttacaa aatgttcaaa atgacgcatg
    48481 aaatcacgtt tcactttcga attatgagcg aatatgcgcg aaatcaaaca attcatgttt
    48541 ttactatggc taaatggtaa aaaacgaact tcagagggat aacaatgaaa actcgcgact
    48601 cgcaatcaag tgacgtgatt atcattggcg gcggcgcaac gggagccggg attgcccgcg
    48661 actgtgccct gcgcgggctg cgcgtgattt tggttgagcg ccacgacatc gcaaccggtg
    48721 ccaccgggcg taaccacggc ctgctgcaca gcggtgcgcg ctatgcggta accgatgcgg
    48781 aatcggcccg cgaatgcatt agtgaaaacc agatcctgaa acgcattgca cgtcactgcg
    48841 ttgaaccaac caacggcctg tttatcaccc tgccggaaga tgacctctcc ttccaggcca
    48901 cttttattcg cgcctgcgaa gaagcaggga tcagcgcaga agctatagac ccgcagcaag
    48961 cgcgcattat cgaacctgcc gttaacccgg cactgattgg cgcggtgaaa gttccggatg
    49021 gcaccgttga tccatttcgt ctgaccgcag caaacatgct ggatgccaaa gaacacggtg
    49081 ccgttatcct taccgctcat gaagtcacgg ggctgattcg tgaaggcgcg acggtgtgcg
    49141 gtgttcgtgt acgtaaccat ctcaccggcg aaactcaggc ccttcatgca cctgtcgtgg
    49201 ttaatgccgc tgggatctgg gggcaacaca ttgccgaata tgccgatctg cgcattcgca
    49261 tgttcccggc gaaaggatcg ctgctgatca tggatcaccg cattaaccag catgtgatca
    49321 accgctgccg taaaccttcc gacgccgata ttctggtgcc tggcgatacc atttcgctga
    49381 ttggtaccac ctctttacgt attgattaca acgagattga cgataatcga gtgacggcag
    49441 aagaggttga tattctgctg cgtgaagggg aaaaactggc ccccgtgatg gcgaaaacgc
    49501 gcattttgcg ggcctattct ggcgtgcgcc cgctggttgc cagcgatgac gacccgagcg
    49561 gacgtaacgt cagccgtggc atcgtgctgc tcgaccatgc tgaacgcgat ggtctggacg
    49621 gatttatcac catcaccggt ggcaaactga tgacctatcg gctgatggct gaatgggcta
    49681 ccgacgcggt atgccgcaaa ctgggcaaca cgcgcccctg tacgactgcc gatctggcac
    49741 tgcctggttc acaagaaccc gctgaagtta ccttgcgtaa agtcatctcc ctgcctgccc
    49801 cgctgcgcgg ttctgcggtt tatcgtcatg gcgatcgcac gcctgcctgg ctgagcgaag
    49861 gccgtctgca ccgtagcctg gtatgtgagt gcgaagcggt aactgcgggt gaagtgcagt
    49921 acgcggtaga aaatttaaac gttaatagcc tgctggattt acgccgtcgt acccgtgtgg
    49981 ggatgggcac ctgccagggc gaactctgcg cctgccgcgc tgccggactg ctgcaacgtt
    50041 ttaacgtcac gacgtccgcg caatctatcg agcaactttc caccttcctt aacgaacgct
    50101 ggaaaggcgt gcaacccatc gcctggggag atgcactgcg cgaaagcgaa tttacccgct
    50161 gggtttatca gggattgtgt ggtctggaga aggagcagaa agatgcgctt tgatactgtc
    50221 attatgggcg gcggcctcgc cggattactc tgtggcctgc aactgcaaaa acacggcctg
    50281 cgctgtgcca ttgtcactcg tggtcaaagc gcactgcatt tctcatccgg atcgctggat
    50341 ttgctgagcc atctgccaga tggtcaaccg gtgacagaca ttcacagtgg actggaatct
    50401 ttgcgtcagc aggcaccagc ccatccttac tcccttctcg agccacaacg cgtgctcgat
    50461 ctcgcttgcc aggcgcaggc attaatcgct gaaagcggtg cgcaattgca gggcagcgta
    50521 gaacttgctc accagcgggt tacgccgctc ggcactctgc gctctacctg gctaagttcg
    50581 ccagaagtcc ccgtctggcc gctgcccgcg aagaaaatat gtgtagtggg aattagcggc
    50641 ctgatggatt ttcaggcgca ccttgcggca gcttcgttgc gtgaactcgg ccttgccgtt
    50701 gaaaccgcag aaatagagct gccggaactg gatgtgctgc gcaataacgc caccgaattt
    50761 cgcgcggtga atatcgcccg tttccttgat aatgaagaaa actggccgct gttacttgat
    50821 gcgcttattc ctgtcgccaa tacctgcgaa atgatcctga tgcccgcctg cttcggtctg
    50881 gccgatgaca aactgtggcg ttggttgaat gaaaaactac cttgttcact gatgcttttg
    50941 ccaacgctgc cgccttccgt gctgggcatt cgtctgcaaa accagttaca gcgccagttt
    51001 gtgcgccagg gtggcgtgtg gatgccgggc gatgaagtga aaaaagtgac ctgtaaaaat
    51061 ggcgtagtga acgaaatctg gacccgcaat cacgccgata ttccgctacg tccacgtttc
    51121 gcggttctcg ccagcggcag tttctttagt ggcggactgg tagcggaacg taacggcatt
    51181 cgagagccga ttctcggcct tgatgtgcta caaaccgcca cgcggggtga atggtataag
    51241 ggagattttt ttgcgccgca accgtggcag cagttcggtg taaccactga tgagacgcta
    51301 cgcccgtcac aggcagggca aaccattgaa aacctgtttg ccatcggttc ggtgctgggc
    51361 ggatttgatc ccatcgccca gggatgcggc ggcggtgttt gtgccgtcag tgctttacat
    51421 gccgctcaac agattgccca acgcgcagga ggccaacaat gaatgacacc agcttcgaaa
    51481 actgcattaa gtgcaccgtc tgcaccaccg cctgcccggt gagccgggtg aatcccggtt
    51541 atccagggcc aaaacaagcc gggccggatg gcgagcgtct gcgtttgaaa gatggcgcac
    51601 tgtatgacga ggcgctgaaa tattgcatca actgcaaacg ttgtgaagtc gcctgcccgt
    51661 ccgatgtgaa gattggcgat attatccagc gcgcgcgggc gaaatatgac accacgcgcc
    51721 cgtcgctgcg taattttgtg ttgagtcata ccgacctgat gggtagcgtt tccacgccgt
    51781 tcgcaccaat cgtcaacacc gctacctcgc tgaaaccggt gcggcagctg cttgatgcgg
    51841 cgttaaaaat cgatcatcgc cgcacgctac cgaaatactc cttcggcacg ttccgtcgct
    51901 ggtatcgcag cgtggcggct cagcaagcac aatataaaga ccaggtcgct ttctttcacg
    51961 gctgcttcgt taactacaac catccgcagt taggtaaaga tttaattaaa gtgctcaacg
    52021 caatgggtac cggtgtacaa ctgctcagca aagaaaaatg ctgcggcgta ccgctaatcg
    52081 ccaacggctt taccgataaa gcacgcaaac aggcaattac gaatgtagag tcgatccgcg
    52141 aagctgtggg agtaaaaggc attccggtga ttgccacctc ctcaacctgt acatttgccc
    52201 tgcgcgacga atacccggaa gtgctgaatg tcgacaacaa aggcttgcgc gatcatatcg
    52261 aactggcaac ccgctggctg tggcgcaagc tggacgaagg caaaacgtta ccgctgaaac
    52321 cgctgccgct gaaagtggtt tatcacactc cgtgccatat ggaaaaaatg ggctggacgc
    52381 tctacaccct ggagctgttg cgtaacatcc cggggcttga gttaacggtg ctggattccc
    52441 agtgctgcgg tattgcgggt acttacggtt tcaaaaaaga gaactacccc acctcacaag
    52501 ccatcggcgc accactgttc cgccagatag aagaaagcgg cgcagatctg gtggtcaccg
    52561 actgcgaaac ctgtaaatgg cagattgaga tgtccacaag tcttcgctgc gaacatccga
    52621 ttacgctact ggcccaggcg ctggcttaaa ctcctttctg atgcccggta agcatgtggt
    52681 taccgggcat ttttgcgtac acgattccgt gcccaatgta tgcgttgcaa cgcagtgaaa
    52741 attcctctga aaacgtctcg caaaggctga aactggcaga tgtcaaaggc ctgggataac
    52801 cgtaatgtcg cgtcatcata aatatcaggt gacggacaac catgaccgaa tcaacaacct
    52861 cctccccgca tgatgcggta tttaaaacct ttatgttcac acccgaaacc gcacgggatt
    52921 ttctcgaaat acatttacca gaaccactgc gcaagctttg caacctgcaa accttacgcc
    52981 tggaacccac tagttttatt gaaaaaagtt tacgcgctta ctactcggat gttttgtggt
    53041 ccgtggaaac cagcgacggt gacggctata tctactgcgt gattgaacat caaagctctg
    53101 cagaaaagaa tatggctttt cggctaatgc gctatgccac tgccgccatg cagcgtcacc
    53161 tggacaaagg ctatgacaga gtcccgctgg tggtgccgtt gctgttttat catggcgaaa
    53221 cctcgcctta cccgtactca ctcaactggc tggatgagtt tgacgatccg caacttgccc
    53281 ggcagttgta caccgaagct tttccgttgg tggatatcac catcgtacct gacgatgaga
    53341 tcatgcaaca tcggcgtata gctctgctgg aactgattca aaagcatatt cgcgaccgcg
    53401 atttaatcgg catggtcgac aggatcacca cgcttttggt tagaggcttc actaatgaca
    53461 gccagctaca aacactgttt aattatctgc tgcaatgcgg cgatacctcc cgtttcaccc
    53521 gttttattga ggagattgcc gaacgttcac cactacaaaa ggagagatta atgactattg
    53581 ctgaacggct acggcaggaa gggcatcaaa ttggctggca ggaaggtatg catgaacaag
    53641 ccattaaaat tgctttgcgc atgctggagc agggctttga acgtgagatt gtgctggcga
    53701 caacccaact cactgatgct gatattccga actgtcatta aaaggagaga ttaatgacta
    53761 ttgctgaacg gctgcggcag gaaggacatc aaattggctg gcaggaaggt aaattagaag
    53821 gtttgcatga acaagccatt aaaattgctt tgcgcatgct ggaacagggc tttgatcgtg
    53881 accaggtgct cgcggccacc cagctaagcg aagccgatct ggcagcgaat aaccactaat
    53941 taacacaggc ccacagccga tccccatggg cctttgatat caataactac cttttatgcg
    54001 tggcccattt ttgcctgatt taaacatcgc cagtcgttga tccagggcat cgctgtagag
    54061 catcgtgtca acgccaacag cgacaaagtt cgctccccac gccaggcatt gctgcgccat
    54121 atcaggagcc acagccagaa aaccagccgc tttacccgca gcacggatcc gccgaatact
    54181 ggtttcaata attcgctgca cttccgggtg cccggcgtta tccgggtagc ccaacgacgc
    54241 agaaagatcc gcaggtccaa taaacacgcc atcaatccct tcgacgtcga ggatttcgtc
    54301 caggttatcc agtgccgttt tactttccac ctgcaccaac agacaaagcg aatcgttaac
    54361 ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc cgtgccacac tggccccgac
    54421 accacgctca ccgtagggag gatagcgcgt ggcagacacc acctgacgtg cctgttcggc
    54481 agtatcgacc atcgggatca gtagagtttg cgcgccaata tccaggactt gtttaatcag
    54541 cggtttactg ccttccaccg gacggatcac gggttggctg gcatagggcg ctaccgcctg
    54601 tagctgatga taaagatcct gaatggtgtt tggcgcgtgc tccccgtcaa tcagcaacca
    54661 gtcataacca gaagtggcgg caatttctgc catataggca gtcgttgagc ttaaccacag
    54721 accaatttgc acttcgccct tgcgtaaacg ttctttaaag ggattgctta ataatgcgtt
    54781 catcgtgttc cttaacctat ttaatgatgt gccacgtcgg tctgcgcaac agtgcgattt
    54841 acacgcagag tgaaaataat cagcgaaccg atgaccgcca ccgctgccag cgtcagtaat
    54901 cccgccgcat cgctggcaaa taacgtttct gctttcacgc gcaggatcgg ggcaataaac
    54961 ccgcccactg caccaaacag gtttacaaaa ccaattcccg cagccagcgc cgtaccggaa
    55021 agcagttgtg tcggcatcgt ccagaacact ggctgcacgg caataaaccc gatagctgca
    55081 acacacagcg ctacgatcgc cattactgga gaaagcagcc cggacagacc aataccaatg
    55141 cccgccgcca gtaatgtcag cgctgcgaca ttacgccgtt cgccggtttt atcggaatag
    55201 cgcggaataa gccaggtccc aaacaaggcc gcaacccacg gaatggcggt gaccaccgac
    55261 gctgtaaagc ccacttttgt tcccagcaat gccgcaacct gggtcggcag gaagaaaatt
    55321 aatccgtaaa ccgccacctg aatggtcagg tagataatcg ctagttgcca gactcggcca
    55381 ttacgcagcg catcgctcag ccgagaagtc actttctgtt gttcttcact tgccagttga
    55441 ttgataagca acgtttttct tgtttactca gaaaacgtgc ctgctccggt gtgtcatcaa
    55501 gccaaaagaa tgtgaatacc ccagcaccga ctgccaacaa tccttcaatc acaaacatcc
    55561 agaaccagcc gggatgcccc ataaatccat gcatctccaa cagcgcgccg gaaagcggtg
    55621 atcccagtgt taacgccagc ggtgcgccca tatagaacag ccccataatg ctggcgcgat
    55681 tacgctgcgg aaaccattgc gaggtgagat aaatcatacc agggaaaaat ccggcctccg
    55741 cagcaccaag cagagtgcga actatcagaa atttcgcttc agtatcggcc catgccatgg
    55801 ctgccgaaag aaatccccac agcagtgttg tcgtaccaat ccaggttctg gcccccagtt
    55861 tgcgcatcaa aagattcgcc ggaacaccca gaaacgcata taccacaaag aaaatgcctg
    55921 ctcccagcgc ataagcttca ttactcaacc cggtatcaat ctggtaggtc tgtttggcaa
    55981 aaccgatatt cgaacggtcg agaaacgcca gcacatacag cgccaacata aacggaatta
    56041 aacgcacacg gtttttcttc accacggcgt caagcaaagc ggtgctcata aaaagctcct
    56101 tagaatatgt gggcgttacc gttgtcacag caacgcccgg agtgattagt ggctgtaggg
    56161 gcgtttcaaa ttgcagtcac gattgagttc gacgccaaaa ccgggtttat caagcactga
    56221 tttatgaata cgaccattca ccggaaccgg ctcattgagc agaatcgggt caaactgcgg
    56281 acgcatcgtt gaacaatccg ggctggtcat caggaattcg ctgaatggcg tattggtgaa
    56341 ggtgatcacc gcatggtgag agtaaacaga cgaaccgtgc ggcaccacca gttgccccct
    56401 ggatttggcg attgcggcaa tttccaccag cgtggttaaa ccaccgcacc agccaacatc
    56461 cggctgcata atgtcgatac cggtttctga aagcgtacga aaagattgca gtgtgccgtg
    56521 gtgctcaccg ctggtgacca tcattccgac tggcgcgttg cgtttcagtt cgcgataact
    56581 ttcatactgc tgtggcggca ggcactcttc gatccatttc aggttatagg gcgcgcaagc
    56641 gtgggccagt ttggtcgcat agttcacgtc ctgactcatc cagcagtcga gcattaacca
    56701 gaaatcctca ccgcattttt cacgcatatc cgcgaccata gcggcatctt tgcggatccc
    56761 cgcatcgcca tcatgtggcc cccagtgcgt cggcattttg ccaccgataa agcccatctc
    56821 ttttgccaga tccggacgcg cacctgtggc gtagaactga atctcatcac gaacagcgcc
    56881 gcctaaaagt ttataaaccg gaagcccgac cactttgccg aacagatccc acagagccag
    56941 atcgacacaa gaaatcgtat tcatcaccag gccaccagag ccggagtaat acagggtggc
    57001 actgagcatt tgatcgtgga tcagtttgat atcactgaca catttaccct caatgaaacg
    57061 gttaagatgt ttttcgacaa taaaacagcc catttcaccg gctgtcgaaa cggcgaatcc
    57121 ggtctggccg ttttctgctt cgacttcaac aaccaacgtg ccaagaacgt taatgccaaa
    57181 tgactggcgt gactgctcgt aatcgcggta tttactcatc ggggtggcaa tatgatcgtc
    57241 tatccagtga ttcgccccct ggtcgtgata atcaccgccg ccagcgcctt tttctgctgt
    57301 cgcaccgcca gtaaaccagg cgcgaacctg tttaattttt ggtagggtca tgatgttctc
    57361 cattgttatg aggcttgtaa gtcaaaggga cttttccatc ccaacagacg tgaaatatcc
    57421 ctggcgcagg caatggcctt gcccgccaga taatcacggt attcttcatt gatttgtaag
    57481 cgggtaccga ccaccgagat cgcagcggta agctcgttat tggcgttaaa caccggcgca
    57541 gcgacacaac ggacatcggc gtaatcttcg ccgttgtcat agctccagcc ctgacggcga
    57601 atacgcgcca gttcttcgtg aagttgctgt ggatgagtaa tcgttgtggg tgtcgcctgc
    57661 tcccagacca gcccttcgat aatactttgc tgtaccgctg caggttgcca ggcaagcagg
    57721 catttaccta ttccggagcg ataaagcgaa aggctttttc cttcatggga acgcacgctg
    57781 atagtggctg atgactccac tttcaaaatg taataagcgc tgccgttatc aataatcccc
    57841 aggtgacata acagcccggt ggtatccatc agttgcgtca aacgcggtcg agccagttcc
    57901 cggagatcca tcttgcttaa cgcatgaccg gaaagctcca ccagcctggt ccacaaacaa
    57961 aaattttcct ggtgatcaag gctaagaaaa cgctgacgcc tgagttcatt aagcagcaaa
    58021 taggccgtgc ttttagggat tcccaatgtg tcaatgatcg tcgcagcact acaggggcca
    58081 atgcgggcaa tcaaattgag aatatcaata gcccgtgtca gggcgggaac tttgcttgat
    58141 tccaacatac tggactccag tcttagatac tggaatcagt ctcgttgctc agggggagta
    58201 aaattgtgaa gcgcatcaaa tcccgctcct cgtccagcag actggagaaa ttacgcacaa
    58261 atttgtgaat cagcgcacgg attttttctt attgagtaca acaggttgga caaattttgc
    58321 cggaggggga atgtgccggt gcagtaaacc ggcacagagt ggcggaatca gacagataag
    58381 gtcatggact caacaacctc aatccagcca tgctcactgg cgatatcctg gccgtttaac
    58441 caacggcgca gcatattcag tgccatcatt gcgcacactt cctgacggat agccaggctg
    58501 tagcgcgtag tgctgaaacg cacacgcaga gcgaaagtgc cgtctggcgt ggctagcgca
    58561 aagttgagat gctcgttctc gaaacccgaa acagccagtg ccagcccggc aaaatggttg
    58621 gcccgccgtt ctgtaatcca gtgcgcagtt tgcgccaggg tttcctcctg tgaaggaacc
    58681 acttcacacg ccagcaatgg agcacctgcg cgagaaagtt gcaaagccaa taaaccaccg
    58741 gtgaattgct cgctcaacgt caggctgaac tggcggtttt gcaattcgcg actgatctgc
    58801 gcgggcagtc cttcagtgcc ttcgaaaatc acgctctgtc cggcaacacg tttaacatcc
    58861 agccacagtt tttccatcgc ctgttgctcg cttgccggtc cggtgagttt cagttcgatg
    58921 ataggcattg aggagcgata gcccattgtt acgcccggcg gcagttgtag agtgtccagg
    58981 ctttgtgcca gatcgctttc cgaacgacca aaagtagtca aacgcagaca aaccggcggc
    59041 tgcggtaaag aaaaacgctc gcgcaggcgc ggcaggattt cgtgctcgac catcacctta
    59101 aattctgacg gtacgccggg agtaaagaac atcaggcaac gattaagctg cacggcaaaa
    59161 ccacaggcgg tgcctaccgg gttattgata aattcagcac tggcaggcag ctccgcttgt
    59221 ttacggttgc tcggtgccat tactcgtcca cgttcgtgga aatagcgttc catctctttg
    59281 agccaggctt catgcagcac caggccttca ccttttgctg tcgcagcggc gagtgcgctt
    59341 aaatcatcgc tggtcggtcc cagcccgccg ttaacgatca gcacatcggc gtgctgacta
    59401 cgttcgcgaa gaatggtgac taagtcatca aggttatccc ccaccgtatt gcggcgagat
    59461 aatggcaacc cctgatgaaa gaaaaaatcg gccagccagg cagcgttagt gtcaacgatt
    59521 tgcccgtgta acacttcatc cccggtggat aacatttcca cttttaacat ggttatctcc
    59581 tgcttttgtt atcgaatcac tataacgcaa agggagataa ggcgtagtaa gaaactgaat
    59641 ggcgcgacag aatgccgcgc cggaagggat cagaaactgg cgttaacccc aacatacggg
    59701 ccgtcagcca cggcgttgtc gcggttaccg tctttacccg acagattcag gtagcgataa
    59761 cccgcctcaa tactgactgg acgcataatg gtgtaacgcg cgccagcatt cgcttcttca
    59821 taactttgaa taccgctgga gagcgaatcc ggagagtagt aatactcgcc aaacaaacgg
    59881 aagctgttgc caattttcca ctgcaaacca cctcctaccg ctgcggcata accttcatcg
    59941 ccgtaattcg ggttggtgta aacgcctttt ccgccaacgg tcgccattaa cggcccgaga
    60001 ggcagattca accccagccc cacgccagcg acatcaccgt cgtcgtcgtt atgtgtccag
    60061 ttaccgctta acgccaggcc cgtcgtttca gtcccaaaac cgacaccaat attggtgtat
    60121 tctttacccg cctgaccgct gatgctcatt gcattgaccg atgcagaaac caacagcatt
    60181 ccggccagac ctgctagcgc aatttttttc attatcccct ttcccacaag caattaatca
    60241 aaattcccaa agctgcggga ttgtactgcc tggcacagag gattcaatga gcgaaaaaaa
    60301 ggatctagtc acttattgta actaaatact tcttaaataa ttaaatgccg aaaaaataaa
    60361 cacactttat cgatacacac aaaaataata ataagtacag ttatcatcgc aaaagagatt
    60421 actgtcactt tcacatattt gtgagttacc gtattctcat ggtttctttt tatttataag
    60481 gaatatccct gtgcgacaac ggactattgt atgccctttg attcaaaatg atggtgctta
    60541 tttgctgtgt aaaatggccg acgatcgcgg cgttttcccc ggtcaatggg cgatttcggg
    60601 tggcggcgtg gagcctggcg aacgaattga agaggcacta cgccgcgaaa ttcgcgaaga
    60661 actgggagaa cagctgcttt tgacagaaat cacgccgtgg accttcagcg atgatattcg
    60721 caccaagacg tatgcagatg gtcgcaagga agagatttat atgatttacc tgatttttga
    60781 ctgcgtttct gccaaccgag aagtgaaaat aaacgaagag tttcaggact acgcgtgggt
    60841 aaaacctgaa gatctggtgc attatgattt gaatgtcgcc acccgaaaaa cgttacgttt
    60901 gaaaggtctt ctgtaacaat gatgacagcc attactggct gtcagattga aaatttagtg
    60961 gttaacgaat tccccatcca gataaacttt gcctttttcc acatgcatga ctaaaccatc
    61021 cagataatca ggtttaaatg tcgcgtcacg cttatcttta gcaatatatg tcaggcaatg
    61081 attatgggtg aaaataacga tatttttatc aggcgctttg ctttgtaagt ccttaattgc
    61141 actataaatc tcattacctc actgaagaag tcgtttatct accgtcaatt ttttacccgc
    61201 tgaaaaccag gtagccgact gaatggtccg gacggtatta ctggaataaa gatcgaaatc
    61261 agggatatca gcactaaaag cgttgcccag ttcacgggca tcctgggtac ctttaaccgt
    61321 aatacctgtt ttatctgaca agcattgatt ggttgaacgg tcgcaacgtt cagcatgacg
    61381 aaacaaaacg acaaccgggt gctgctgtgc cagtctggcc agtgttttat tgtcgatacg
    61441 tggcaaacca ttgctactcc aggcggcatg agtacccagt ccggcaattg cagcgagcgc
    61501 cagtaaaatg atgatatatt tttttgactt caacgaagag cggcaaaaag ctaacacgta
    61561 aactccacct atagacaagc gcaaccagac aattaccgtg aaattgagct acatttctgg
    61621 cgataattcg cagttggtgt aatattaaaa atcctacgat gtcggcaaaa tgcctcaaaa
    61681 ttttgccaaa tgcaaagcct aaataagaaa aaatataaaa atttcaatat ttacgtctaa
    61741 tattagtttc ttaaggttaa gttaatattc tatccttaaa atttcgatcc aaatggcaaa
    61801 atatacacaa cactctttat agcaaatata agtggacagg tattcaatgg cggaaggaaa
    61861 agcaatgtca gaatttttgc ctttttcgcg accagcaatg ggcgtggagg aactcgctgc
    61921 agttaaagag gttctcgaat ccggttggat cacaaccggt ccgaagaatc aggcgcttga
    61981 gcaagctttt tgccagttga cgggaaatca gcatgccatc gcggtcagtt cagccaccgc
    62041 cggaatgcat atcacgctaa tggcgttgaa aattggcaag ggcgatgaag tgattacgcc
    62101 ttccctgacc tgggtttcaa ccctcaatat gatttccttg ttgggtgcaa cgccggtaat
    62161 ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa gctatcgagt cagccattac
    62221 gccacgcact aaagccatca ttccggtgca ttatgccggt gcgccagcag atattgacgc
    62281 cattcgcgcc attggcgaac gttacggcat cgcagttatc gaagatgctg cccatgccgt
    62341 cggtacgtat tacaaagggc gacatattgg cgcaaaaggt accgctattt tttcatttca
    62401 tgccattaaa aatattacct gtgctgaagg tggcctgatt gtaactgata atgaaaacct
    62461 tgcccgccag ctacggatgc tgaaatttca cggtctgggt gtcgatgcct atgacagaca
    62521 aacctggggc cgtgcaccgc aggctgaagt cttaacaccg ggctataagt acaatctgac
    62581 cgatattaac gccgcgattg ccctgacaca gttagtcaaa ttagagcacc tcaacacccg
    62641 tcggcgcgaa attgcccagc aatatcagca agcactggca gctctcccct ttcagccatt
    62701 aagccttccc gcctggccgc acgttcacgc ctggcatctg tttattattc gtgtcgatga
    62761 acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaaa gaggcattgg
    62821 taccgggtta catttccgcg ccgctcacac acaaaaatat tatcgcgagc gttttcccac
    62881 gctgtcgtta ccgaataccg aatggaatag cgaacgcatc tgttctttgc cgctgttccc
    62941 ggatatgact accgccgatg ccgaccatgt catcacagcc cttcagcaac tcgcaggaca
    63001 ataagccatg tttgaaatcc accctgttaa gaaagtctcg gtggttattc ccgtttataa
    63061 cgagcaggaa agcttaccgg aattaatcag gcgcaccacc acagcctgtg aatcgttggg
    63121 gaaagagtat gagatcctgc tgattgatga cggcagtagc gataattccg cgcatatgct
    63181 ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct attttgctta accgcaatta
    63241 cgggcaacat tcagcgatta tggcgggatt cagtcacgtt actggcgact taattattac
    63301 ccttgatgcc gatctccaga atccgccaga agaaatcccc cgcctggtgg caaaagccga
    63361 tgaaggttac gacgtggtag ggactgtacg ccagaaccgc caggacagct ggtttcgtaa
    63421 aaccgcttcg aagatgatta accggcttat tcagcgcacc actggcaaag cgatgggtga
    63481 ttacggttgt atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt tgcactgcca
    63541 tgaacgcagc acctttatcc cgattctggc gaatatcttc gcccgccgtg ccattgaaat
    63601 tccagtacat catgccgagc gtgagtttgg tgaatccaaa tacagtttta tgcgcctgat
    63661 taatttgatg tacgacctgg tgacctgcct taccacaacg ccgctacgta tgctgagtct
    63721 gctcggcagc attattgcga ttggaggttt tagcattgcg gtgctgctgg tgattttacg
    63781 cctgaccttc ggaccacaat gggcggcaga aggcgtcttt atgctatttg ccgtgctgtt
    63841 tacttttatt ggcgctcagt ttatcggcat gggattactc ggtgaatata tcggcaggat
    63901 ctacaccgat gtccgcgccc gcccccgcta ttttgttcag caagttatcc gtccatccag
    63961 caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc acgatatggg atgcctcggt
    64021 attgaagccc tgctggctgc cggttacgaa attagcgcca tttttaccca tactgataat
    64081 cccggtgaaa aagcctttta tggttcggtg gctcgtctgg cggcggaaag aggcattccg
    64141 gtttatgcgc cggataacgt taatcatccg ctgtgggtgg aacgcattgc ccaactgtcg
    64201 ccagatgtga ttttctcttt ttattatcgc catcttattt acgacgaaat tttgcagctc
    64261 gctcccgcag gtgcatttaa tctgcatggt tcgctgttac caaaatatcg tggtcgcgcg
    64321 ccgctgaact gggtgctggt caacggtgaa acggaaactg gcgttacatt gcaccgaatg
    64381 gtgaaacgtg ccgatgccgg ggccattgtg gcgcaactgc gcattgccat tgcgccagac
    64441 gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct actggaacag
    64501 acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga aaacgaagcc
    64561 acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa accggcatcc
    64621 gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt
    64681 ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag
    64741 ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg cgcgctggaa
    64801 atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt agcgcagacg
    64861 ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc ccgacgccgt
    64921 acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac agaacgcctg
    64981 ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc gataagccgt
    65041 tttctgaatc atccgcattt tcactttgtt gaaggcgata tcagtattca ttccgaatgg
    65101 attgagtatc atgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat agccacgccg
    65161 attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga gaatctgcgc
    65221 attatccgct actgcgtgaa gtaccgtaag cgaatcatct tcccgtcaac ttcagaagtt
    65281 tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat cgtcggcccg
    65341 gtgaataaac cacgctggat ttattcggta tcaaaacaat tacttgatcg ggtgatctgg
    65401 gcctatggcg aaaaagaggg tttacagttc accctcttcc gcccgtttaa ctggatggga
    65461 ccacgactgg ataaccttaa tgcagcgcga attggcagct cccgcgctat tacgcaactc
    65521 attctcaatc tggtagaagg ttcaccgatt aagctgattg atggcggaaa acaaaaacgc
    65581 tgctttactg atattcgcga tggtatcgag gcgttatacc gcattatcga aaatgcggga
    65641 aatcgctgcg acggtgaaat tatcaacatt ggcaatcctg agaacgaagc gagcattgag
    65701 gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca tcatttccca
    65761 ccgtttgcgg gctttcgcgt tgtcgaaagt agcagctact acggcaaagg atatcaggac
    65821 gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg ggagccgaaa
    65881 attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac cgttgatctt
    65941 acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatacc tttcgtggca
    66001 cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt caggccagca
    66061 tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg gtgaagccac
    66121 agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg gatattttac
    66181 tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat atcattcgtg
    66241 aagcggctaa acatcacgaa gtcggcctgc acgcctggga tcaccatgcc tggcaagccc
    66301 gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt cttcgcactc
    66361 tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg cgtgccgacc
    66421 agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg
    66481 ccatgccgtt ccgtccattg ctcgaatcag gaaaccctgg cactgcgcaa attccggtga
    66541 ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat tttaacggtt
    66601 ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt catgcagaag
    66661 ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca gctcaggaag
    66721 gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc ggacaagttg
    66781 ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc
    66841 gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg tttattgcct gctattacct
    66901 gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg cggaaatcag
    66961 tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc tacgttattt
    67021 cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat ttggcgcgaa
    67081 taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg cgctggtgac
    67141 ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca cagtaattta
    67201 tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc cgtttattgc
    67261 attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac agacgtggaa
    67321 aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg tgatgaccaa
    67381 aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag cgacgcaaaa
    67441 acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg tactgacggt
    67501 tctcccttgg ggactggcga tagcgcagcg ggagcctaac ttctggcact attttttctg
    67561 ggttgagcat attcaacgct ttgcactgga tgatgcccaa catagagctc cgttctggta
    67621 ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc ccggtgcact
    67681 gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga gctggacgat
    67741 aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg cccacctata ttctttcctg
    67801 ctttgcatct ctggcaatgc tgatggcgca ttacgctttg ctggcagcaa aaaataatcc
    67861 tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg gcattattgc
    67921 cacatttgtg gtctccccgt ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag
    67981 ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg gctggtacac
    68041 cttaacaaac gtcgaaaaga cctggccttt tgccgcgctt tgcccgctgg ggctggcgtt
    68101 gctggtagga ttttcaattc ctgacagagt tatggaagga aaacatccgc aattttttgt
    68161 cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata gcgtcggtgt
    68221 tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc gccagacagg
    68281 tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca gcggtgatga
    68341 gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attactctcg tgctttcggt
    68401 tgaccgcgat gaagatatca acagtctcgc gattccgccc gcagatgcca tcgatcgtca
    68461 ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt agtctttgcc
    68521 agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt tgtggcgata
    68581 aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt
    68641 gccatggtgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc ttacccgatg
    68701 ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca cgaaccggta
    68761 tcgccgcgtc actggtgtgg ggtggcgttc attattggcg gcattgtgat cctcgggagt
    68821 acggtgtaat gggcctgatg tggggattat tcagcgtcat tattgcctca gttgcgcaat
    68881 taagcctggg ttttgcggcg agtcatctgc cgccgatgac gcacttatgg gattttatcg
    68941 cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg ctgggttatc
    69001 tgctttccgt tttttgttgg tataaaacgc ttcataaact tgcgctaagc aaagcctatg
    69061 ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt cccggctggg
    69121 aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc gggttgatgc
    69181 tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccact ttacactgcc
    69241 gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta tgcagctttt
    69301 cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc actttcacgg
    69361 cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg cagagcataa
    69421 gtacatgtct gttatcttgt ttattgcagc acgatttttt gaccagccat tccattgcat
    69481 tatcctgttt gctaagagtt ttcacatcaa tttacagatt aacagataaa ctctttatca
    69541 acggcaatgg aatggcagaa atgagaagtg aaacggaggg ttaattattg ctgacgttgc
    69601 acccactctt ttagcgcctg acgtgaaatt ttaataccgc cgtttttcag ctccggcggc
    69661 agagttagcc agcgcaccgg ttgttgaaaa cgggccagct tatctttcac ccattcacta
    69721 agatcaacgc tctcgtggtc atactccatc accgccaccg gtcgatgacc aaactccttg
    69781 tcggcaacgg ggacgataaa cacctgcaaa accgcaggat gtgcagcaat tacgcgctcg
    69841 acttcttccg gctgaatacc ctctccgcca ctgaagaata gattgtctaa acgtccgaca
    69901 atggtcagct tgccattatg catctcaccg cgatcgcgcg tagcgtacca gccttcgtca
    69961 ttaaccagtg aaaccagttg cccgttacgc cagtaacctt ctgccatact ggcagcccgc
    70021 agccacactt cattattaac gattttcact tcccgacccg gcagcggcga accaacgtct
    70081 gccaggccgt cggcttcttt cgcacacacc gtggaggcaa actcggtcag accatagccg
    70141 caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg gatagccgcg
    70201 ccgccaagta acaccgcttt cagggaaacg gaactacggt taaccagcaa acgccagagt
    70261 tgtgttggca ccagtgaagc gtgagtacag cctgccagca tttgctccaa tggctgttta
    70321 tcacgtaccg tcatccgcgc accagcgtat aaccagcgcc acataattcc ctgaccggag
    70381 acgtgaaaca gcggtaaaga gagcaaccaa tcatcgtgat cgccaaacgg aatcagcgat
    70441 aacacacctt gcgcactggc aagatgggcc tgataagtat ggacagcggc tttcggcaaa
    70501 ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg tcggctgcca cgtagcggca
    70561 tgtgcgcctt caaccagctg aatgtgcagc gacgttaatg ccggaaacgt gttttcccca
    70621 tccggcacca gagcaaattg cagcgtcaga ttgggcagca attcttcaag caacggttgc
    70681 ggcagctgag ggttcacggg caacacccgc gccccgcatt gcagtaacgc cagccaggcg
    70741 agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc cgctgccctc aaccaccccc
    70801 tgcaccgcaa atccggaggc taattcatcg acgcgagcac aaagctcgcg ccagttgagt
    70861 tgctcgtcat taagacgtaa ggcgatggtt tctccccgca cttgccgcca gtgacgccac
    70921 ggccagtcag agaagatcat aacaaccgct ccagtgcatc aacttccacg acaggcagcg
    70981 tgctacccgg ccagcgacgt acctgctgcg cctgcatcag atccagcgtg tccagccctg
    71041 gaatggtgtc cggcgttaac caggcggcaa tccgcgccag ttgcgttaag cctaagctcg
    71101 attcaatgga agaactgatc accgccgtca gccccagcgc gtgcgccgcc tgtacctgct
    71161 cgcgtacttt ttccagactg cccgtgagcg tgggtttgat aactaccgcg cgcacgccct
    71221 cttcagccac aaaggcaaaa tccggctcgc gcaggctttc atcccaggca atggcaatgc
    71281 cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg
    71341 cgatgcggtc gcgataatcc gggttaacgt atttggcaaa ctgctgacct ttcagcggtg
    71401 tccaggcgcg atttgcgtca agacgcaaat gcagatccgg aattgcctcc agcaacagat
    71461 tcaccaccat gccgtcgcgc accgcttcgt acaatcccac tttgaccttc gccactttct
    71521 cgcctggcat atctgcaagt ttgaggatca gatcgtccgg atcgccatta cacagcggtg
    71581 ccgcacggta gttggctgct tgcggcaacg tatctgtcag ttctgccaat gcacagctta
    71641 cgccaaaggc cacggaaggc atctgcggta gctcgcaatc gcctgccagc cagttattta
    71701 cccaggcaag cagcacactt tgcgcctctt cccaggtttc ctgactgaag cccggcagtg
    71761 gggagatctc cccccaccct tcgcgctcgc cttcacgcag gcaaacatac agcccgtcgc
    71821 gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc catggggatc tgccagcggt
    71881 atacctgcgc gctacgcatt acggattccg tttgaatttg ctgaagtcag gctgacgttt
    71941 ctggttgaag gcgttgcgac cttcctgacc ttcttccgtc atgtagaaca gcatggtggc
    72001 gttgcccgcc agctcctgca gccccgcctg cccgtcacag tcggcgttca gtgcagcttt
    72061 caggcagcgc agcgccatcg ggctgttttg cagcatttcg cggcaccaac ggacggtttc
    72121 tttttccaga tccgccagcg gtaccacggt gttcacaagg cccatatcca gcgcctgttt
    72181 tgcgtcgtac tgacggcaca ggaaccagat ttcacgcgct tttttctgcc cgacgatgcg
    72241 agccatgtag gaagcgcccc agccgccgtc gaaggaaccg actttcgggc cagtctgacc
    72301 gaagatggca ttatctgccg cgatagtcag gtcgcacatc atgtgcagaa cgtgaccgcc
    72361 gccgatggag tagccagcca ccatcgcgac aaccggtttc ggacaggtac ggatctgacg
    72421 ctggaagtcc agcacattca ggtgatgtac gccggaatca tctttatagc cgccgtaatc
    72481 accacgcact ttctggtcac caccggagca gaacgcttta tcgcctgcac cagtcagaat
    72541 gatcacgccg atgttgtcgt cataacgcgc atctgccagc gcctggatca tctcttttac
    72601 cgtcagagga cggaaggcat tgcgcacctg cggacgatta atggtgattt ttgcgatacc
    72661 gtcggtggat ttttcataac gaatgtcctc gaaaccttcg gagcagtcgt gccattcaac
    72721 cggtgcgtaa agcattgctt catcaggata aatcatattg tgtcctttag tcagaaacgc
    72781 aagatctgcg ccagacttgc gattacgcca gcgggatttt cccgatgcgc gttatgtccg
    72841 gcgcgaggaa tgacatggca gtcggcagcc agttccgccg ccagggcgcg gaatttgctg
    72901 tcacgttcac cacataaata ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa
    72961 tcaggctgga cggcgagaga agtcgcctcc agcatggcgg caagcgttgc gccattattg
    73021 ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat tgagtgaggc aaaaacaggc
    73081 tgttgatacc agtcggcaaa taccgccgtt aacggttctg tgagaaaacg ctgcacccat
    73141 tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat tttgcagccc cggatgcccg
    73201 ccttcgacaa taaccccaca aagccccgcc agcccctggc aagccgccat catcgccacc
    73261 cgtccaccaa gcgagtaccc caccagccag aagtcaagga tgttgtaact aaccaaggtt
    73321 ttacgcagta agtcggtgac atcatcaaat ccatcgacgc taatcgccgc cgaaccaccg
    73381 tgacctggga gatcaacata caaccgtgag tagtcggcaa acgcctcgcc cacttcttgc
    73441 cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa acctggtttt
    73501 ccgtgttttg cctgcgcgtg caggatcata aatggcttac ctgcgccaga agttgctgga
    73561 gcgtttgcgc accatcggtg tcgttaacca ccatttcaat caccgtggtg gttggcgtgc
    73621 gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt ttgcggacga tgatatttca
    73681 gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg cggcatcaga tagaaacgct
    73741 cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg cccgccgttg ttgttcacca
    73801 caattaatac cagcggcgca gaaacctgac gcaataacgc cagcgcgttg agatcgtaaa
    73861 gtgcggagag atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc tgaacgccgg
    73921 cggcggtcga aagcagcccg tcgataccgc tggccccacg gttgctgtac accgggtaac
    73981 ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac caagctgtta ccaacaaaca
    74041 attgcccctg ttcaggcaga tagtcgcaga tgcgatgcgc cagttgcgct tcgccaaacg
    74101 catcacggcg ggcaataacc gcctgcattg cctgttccgc caggcgcggg atttcaacgc
    74161 accagggctg gcgtttttct gccggatgca gctccagcca gtcggcaata ttggcaatta
    74221 agcgacgtcc gcgatggtgt gccggatcaa gtcgcccttc aatgtcatca acaatccagt
    74281 actcttctgg ttcacagctt gcctgccatt gcaggagccg tttccccgtc aggctgcttc
    74341 ccagttgcac cacaatttgc gcctgctgca gctcgctggt cgctttggca ttgcctaacc
    74401 aaagatcggc acacggcagc ggctgcccgg tttgtgacag cacatcgcca atcagcggcc
    74461 agccaagagt ttgcgcccac agggcaactt ttttgccctc ttccgcactc atgcgcccgg
    74521 caaccaccac gccgcgcttt tgtcgccaga agaaccagtc gcgctgtttt tcactttcca
    74581 gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca ccagtcaccc agacgctgtt
    74641 gccagctaag cccggtatcg tccatttcgc catacagcgg ttcagcaaac gggcagttga
    74701 tatggactcc ccccgcatga agcgtaccga gagcgtggtc gatggtagaa accagccaac
    74761 gtgcggggat atcctgggtc gggcgcggca atgaaatact gtgcgtgggg tgagaggcga
    74821 acattcccgg ctggcgaatt gcctgattcg cgccgcagtc aattagctcc ggcgggcgat
    74881 cggcggttaa gagaatcagt ttttctccgg ttaacccggc ttcaatcagt gccggataga
    74941 gatttgccac cgccgtgccg gaggtcacaa tcaccgccac cggctgcttg ctgactttcg
    75001 ccagccccag cgccagatgc cccaacccac gctcatcgaa atgggtgtgg tgaatgaatg
    75061 cggaattctc cgccgccgct aacgttaacg gtgtagaacg cgagcctggg gcgatacaga
    75121 tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac cgccgcccag cgtcggttaa
    75181 atgcgcttac tgacatgagt ttgtccggta tcaatattgc ggctaagtat aaggagctac
    75241 aaaaatagat tattgatatg aatcggtaat gatgcgactc attactattc catttgtaat
    75301 aaagtacgca gccctgccgc tttgttgtcg atttcctgcc actcttgctc ggggtcggaa
    75361 ccacggacaa tgcccgcgcc agcatataat cgcacgacat tgccgctaat ttttgctgag
    75421 cgcagggaaa cgcagaattc gctttgttgt aatgagagat agcccgcaga accggcgtac
    75481 cattctcggg tgaacggttc gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag
    75541 ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc cgctttgttg
    75601 agtgaagtcc agatacagcg gcgaagatgc tgcactttac gcagacgcag tacctgcggc
    75661 ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt gacagatatc ttccaccacc
    75721 agcatgttct cgcgctggtt tttatcatcc gccatcagcc actctcctaa ctgctgcgcc
    75781 tgcttatcat caggattatt tgctactgtt cccgctagcg cttcagtacg cagcgctttg
    75841 tcacgccgcc gccataaccg ttccggtgaa gagccaagaa aagcattttc gccatcaaag
    75901 gccatgtaaa aatggtagca attcagattc agtcgacgac tggcagccat catcgccgcc
    75961 gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag cgagcaccac tttgtcgagc
    76021 tcaccttcgg cgatggtttt cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc
    76081 ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag gcaagggctt gatactcacc
    76141 agtgtggcga taaattcttt tgcctgaatc gcatcgtgct gaagggagct ttcgctgaat
    76201 agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc attccaggcg gggtaaaagt
    76261 aaattgccct gcgacgggtc aaaagcattc agcccccaaa tgcgtaagtc ggcgtgttcc
    76321 gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt aatcgcgccc
    76381 aggacgacag cttcttcatc accattacgt tgttgccagt agaattgcgg gtatgtttgc
    76441 tgactggcca gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg aatatcgata
    76501 acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag attttccagc
    76561 gccgtagtaa gtgattgcac gcggacctct ccctgttaaa agcctcacat tatacggggt
    76621 actacaaaaa aaatgcagta ccccggtgta gggaggtttt aacggcgtgc cagcaacagt
    76681 cctagtacca gcccaacggc cgcgcccaca ccaattcctt gccagggttt ttcgtggacg
    76741 tagtcatcag cacgataaac tgcctgcttc gcccgatagt aataactgtc tgaagcctgg
    76801 ctaacccgtt ttttcacatc atccagcgct ttttctgcac gcgctttcag ctcaacatat
    76861 ttctgatcgg cgggatcgcc agaggagcgg agcacctctt ccagtgtttc actaagcagc
    76921 gtcaggtcgt catcgatacg tgtatcacca aactgattag acattctcgt tctccatttg
    76981 cgtaaaacct gtcactaact atagacaagg gttgttgatt acgcctgaat tacctcgcgt
    77041 gccatcccaa tgtgtgggat accatcttct tcatagacct ccgtcacagg gataaaaccg
    77101 aaactctggt agaagttttg cagatgcgcc tgcgccccga ggtaaacagg cttatcaggc
    77161 cagtgatgcg tacatgtttc gagtgtttta ctcatcagct gctgccccac tttttcgcca
    77221 cgcaacgctt cactgacaat cacccgacct ataacgaccg gctcaagatc atcatcactt
    77281 ttcagaatcc tcgcatacgc caccagttca tcatttttcc aaccgagaat atggcgatta
    77341 tcccccgtca aatcatcacc atcgatatcc tgataagggc aattctgctc gacaacaaac
    77401 accgcgcaac gcagttgtaa taaggcataa agctgggaaa ccgataattc agagtgatgc
    77461 agatcttgcc attcaatcat aatgctctcc ttttagatgt atgccttata ctagccggaa
    77521 tattttttga aacgtgatga actcatggaa ttaatttttt taggtacttc agccggtgtg
    77581 ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc tgcaacatcc gacccagagc
    77641 ggactttggt tgtttgactg cggtgaaggc acccagcatc agctactgca taccgccttt
    77701 aaccctggaa aactggacaa gatttttatc agtcaccttc atggcgatca tctttttggt
    77761 ttacccggct tgctgtgcag tcgttctatg tcaggcatta tccaaccctt aacgatttat
    77821 ggtccgcaag gtatccgtga atttgtggaa accgcgctgc ggattagcgg ctcatggacc
    77881 gattatccgc tggaaattgt cgaaattggc gctggcgaaa ttctcgatga tggcctgcgc
    77941 aaagtaaccg cttatccgct ggaacaccca ctggaatgtt atggctatcg tattgaagaa
    78001 catgataaac cgggtgcatt aaatgcccag gcattaaaag ctgctggcgt gccgcctggc
    78061 ccactgtttc aggaattaaa agcgggcaaa acaatcacgc tggaagatgg aaggcagatt
    78121 aacggcgcag attacttagc tgctccagtg ccaggtaaag cgctcgctat tttcggcgat
    78181 accggcccct gcgatgccgc acttgacctg gctaaaggtg tcgatgtcat ggtgcacgaa
    78241 gcgacgctgg atataaccat ggaagccaaa gccaatagtc gcggccatag ctctacacgc
    78301 caggctgcga cactagcccg tgaggctgga gtcggcaagc taatcattac ccacgtcagc
    78361 tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg aatgcaggtc aattttcccg
    78421 gcgactgaac tggcgaatga tttcaccgtg tttaacgttt aactctgctt attattaaaa
    78481 acagggcgaa acttgccctg ttatcgcaac ccgcgctaca tatatccacc cacacaggca
    78541 tattgagcga tttttgatat ttattcatct ccttacgcac tatattcaga gcaataagtt
    78601 gcctcttttc atcttgattc agcacaacct ttgcaactaa aaggagccca tgatggttac
    78661 agttgtcagc aattattgtc aattatctca aacgcaactc agtcagacat ttgcagaaaa
    78721 atttactgta accgaggaat tactgcagtc tttaaaaaaa acagcgttat ccggagatga
    78781 agaaagcatt gagttactgc ataatattgc gttaggttat gataaatttg ggaaagaagc
    78841 tgaagatatt ctttaccata ttgttagaac cccaacaaat gagaccctat cgattatccg
    78901 acttatcaaa aatgcttgaa attatataat ctggcacata tcgcaaccaa ctcccccctc
    78961 aaatcacatg attcagatga tctcctgttc aaaaaactat tctccccttc gaaattaatg
    79021 acaattatcg gtgatgaaat tcctcttata tctgaaaaac agtcgctttc aaaggtgctt
    79081 ttaaatgatg agaataatga actgagtgat ggtacaaact tctgggataa aaatcgtcaa
    79141 ttaaccacag atgaaatagc ttgctatctt cagaagatcg ccgctaatgc aaaaaatact
    79201 caagtcaatt atcctactgg tctctacgtc ccctactcca ccagaactca cctggaagac
    79261 gctctcaatg aaaatattaa gagcgatcca tcatggccga atgaagtcca gttattcccc
    79321 ataaatactg gcggacactg gatattagtt tcgctacaga aaatagtaaa taaaaaaaat
    79381 aataaactac aaataaaatg cgtcatattc aactcattgc gtgcactagg ctatgataaa
    79441 gaaaattcac ttaagcgtgt cattaatagt tttaattctg aactcatggg agaaatgtcg
    79501 aataataata taaaagttca tttaaatgaa ccagagataa tatttttaca tgccgatctt
    79561 cagcaatact taagccaaag ttgcggtgca tttgtgtgca tggcagccca ggaagtgatt
    79621 gaacaaaggg aaagcaattc tgacagcgcc ccctatacgt tattaaaaaa ctatgctgac
    79681 agatttaaaa aatattcagc agaagagcag tacgaaattg attttcaaca tcgactggca
    79741 aacagaaatt gttatttaga taaatatggc gatgcaaata tcaatcatta ttatagaaac
    79801 ttagaaataa aacactcaca acccaaaaat agagcatccg gcaaaagagt gagttaacat
    79861 cattgttaat taattcgacc agtcagcaaa tatgctgact ggtcgaacat agtttgctaa
    79921 acagtcatca ctgactgatg tcagtcacac catccgccag ttcaatcagg cgaataaatt
    79981 ccgccctgta accctgtgga tcttcccctt ttgcctgctg agcccactgt ttgatctgct
    80041 gccaggaggt attgttcagg tattcagaac cgcgtaactt ttgcccatat gcagctactg
    80101 ctgcgcgaaa acgcatatct tcagagggcg cgtttattgt tggccccagc gggaattcaa
    80161 ctaactgact ttcttttccc tgcgggtatt tccagcgaat ttttaaccag gccagttctt
    80221 tcgttttgtc cgatttcgct aatttgttat ccggggcata gcgtaactta tcaattgatg
    80281 ctttttgccc gttcagcgtt aattcgaata acaacgttat atgtttgcct gcgcctatat
    80341 cccctgcatc aacgttgtcg ttattaaaat gttccacccg aagttggcgc ttttcataac
    80401 caatctgacg gtattccgtt acccacgcgg ggttaaactc aatttgcgct ttgacatctt
    80461 ttgctacggt aatcaacatc tgccgcattt cactattcaa tactttctgc gcttcagaga
    80521 gggtatcaat gtagctgtag ttgccgttac caacatcggc aattcgcacc atcattgcct
    80581 cgttgtaatt gctattcccc acgccaaacg tcgacagagt aacaccagac tcccgctgtt
    80641 ttttgaccat tgattcaatc gattttggat cgtcaatgcc aacgttaaag tcaccgtcag
    80701 tggctaataa aatgcgattg atgccgccct taataaaccc cttcgtcgcc tgctgataag
    80761 ccagttccag cccggcaccg ccattggtac tgccttcggc atccagggaa tcaattgcgg
    80821 cattaatttc cgccttatga ctcccggaga tagaaggcaa tgcaatacgg gagtcgccag
    80881 cgtaggtcac gatggcaatg ttatcctgct cacgaagttc tttgaccaat aatttcaacg
    80941 aagactggat aagtggcaaa cgttcatcag aaatcattga accagaagtg tcgataagaa
    81001 agaccagatt agaagctggt aactcttcac ttttgcgatc tttcgccagg atatcaactt
    81061 tcagcaatgt tcgctgttca ttccatggtg caggtgccaa ttcgtagcgc atagcgaaag
    81121 gtattggctt agaggccgga atagattgtt tgtctttgat atcccaatca gacgggaaat
    81181 aattgactat ctcctccacc cgcacagcgt ctggcggagg taacagccct tgattgagga
    81241 aacgccttac attcgcataa ctgccagtgt caacgtcaag actaaacgtc gccaacggat
    81301 tttgcgctac ctgcttaacc ggattatcat cgaactgctg gtagcgagcg gttcctggat
    81361 ttgcgatatg tgtagctttt gctttagccg ctcttgcaaa tgttggcgct tcctgcaatc
    81421 gcccctgtaa agcctgtttg tctgaatatt gttgcacttc ttgctgggcc aaggctttcg
    81481 cggcggcggc gctttgctca gcctctttta ttgcagcttg ctgcgcggca agcacttgct
    81541 gctctgtggg agtactgggt tgttgttgct gactttcctt attctcaggt tgcggcccac
    81601 atcctgacaa aatcaaacta ctcataagca acatgattat atttttattt cgcatataaa
    81661 atccttttaa ataaactcag gcaccagaca taaaata
//
LOCUS       DBBGLQ010000013        80600 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.013, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000013 DBBGLQ010000000
VERSION     DBBGLQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.013"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..1600)
                     /locus_tag="ACIYSB_001144"
     CDS             complement(<1..1600)
                     /locus_tag="ACIYSB_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HGC5428229.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLVTAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSSEGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNQGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGK"
     gene            complement(2131..3723)
                     /gene="lsrK"
                     /locus_tag="ACIYSB_001145"
     CDS             complement(2131..3723)
                     /gene="lsrK"
                     /locus_tag="ACIYSB_001145"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGC5428230.1"
                     /translation="MARLFTLSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(3802..4755)
                     /gene="lsrR"
                     /locus_tag="ACIYSB_001146"
     CDS             complement(3802..4755)
                     /gene="lsrR"
                     /locus_tag="ACIYSB_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGC5428231.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTNIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            5004..6539
                     /gene="lsrA"
                     /locus_tag="ACIYSB_001147"
     CDS             5004..6539
                     /gene="lsrA"
                     /locus_tag="ACIYSB_001147"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGC5428232.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIEGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTERDINVETIMRVAFGDSQRQE
                     ASC"
     gene            6533..7561
                     /gene="lsrC"
                     /locus_tag="ACIYSB_001148"
     CDS             6533..7561
                     /gene="lsrC"
                     /locus_tag="ACIYSB_001148"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGC5428233.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            7561..8553
                     /gene="lsrD"
                     /locus_tag="ACIYSB_001149"
     CDS             7561..8553
                     /gene="lsrD"
                     /locus_tag="ACIYSB_001149"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGC5428234.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            8565..9587
                     /gene="lsrB"
                     /locus_tag="ACIYSB_001150"
     CDS             8565..9587
                     /gene="lsrB"
                     /locus_tag="ACIYSB_001150"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGC5428235.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKELGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIKGVGQVEVSPNSVQGYDYEADDNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            9614..10489
                     /gene="lsrF"
                     /locus_tag="ACIYSB_001151"
     CDS             9614..10489
                     /gene="lsrF"
                     /locus_tag="ACIYSB_001151"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGC5428236.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNR
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            10513..10803
                     /gene="lsrG"
                     /locus_tag="ACIYSB_001152"
     CDS             10513..10803
                     /gene="lsrG"
                     /locus_tag="ACIYSB_001152"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGC5428237.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            10860..11618
                     /gene="tam"
                     /locus_tag="ACIYSB_001153"
     CDS             10860..11618
                     /gene="tam"
                     /locus_tag="ACIYSB_001153"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGC5428238.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(11622..12536)
                     /gene="yneE"
                     /locus_tag="ACIYSB_001154"
     CDS             complement(11622..12536)
                     /gene="yneE"
                     /locus_tag="ACIYSB_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGC5428239.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(12743..14194)
                     /gene="uxaB"
                     /locus_tag="ACIYSB_001155"
     CDS             complement(12743..14194)
                     /gene="uxaB"
                     /locus_tag="ACIYSB_001155"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGC5428240.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(14421..15407)
                     /gene="dgcF"
                     /locus_tag="ACIYSB_001156"
                     /pseudo
     CDS             complement(14421..15407)
                     /gene="dgcF"
                     /locus_tag="ACIYSB_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(15480..15839)
                     /gene="yneG"
                     /locus_tag="ACIYSB_001157"
     CDS             complement(15480..15839)
                     /gene="yneG"
                     /locus_tag="ACIYSB_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGC5428241.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(15839..16765)
                     /gene="glsB"
                     /locus_tag="ACIYSB_001158"
     CDS             complement(15839..16765)
                     /gene="glsB"
                     /locus_tag="ACIYSB_001158"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGC5428242.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(16829..18217)
                     /gene="sad"
                     /locus_tag="ACIYSB_001159"
     CDS             complement(16829..18217)
                     /gene="sad"
                     /locus_tag="ACIYSB_001159"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGC5428243.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            18318..19203
                     /gene="yneJ"
                     /locus_tag="ACIYSB_001160"
                     /pseudo
     CDS             18318..19203
                     /gene="yneJ"
                     /locus_tag="ACIYSB_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            19281..20396
                     /gene="yneK"
                     /locus_tag="ACIYSB_001161"
     CDS             19281..20396
                     /gene="yneK"
                     /locus_tag="ACIYSB_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGC5428244.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPARNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLPSDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQE
                     SPHFQNLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            20546..21736
                     /gene="ydeA"
                     /locus_tag="ACIYSB_001162"
     CDS             20546..21736
                     /gene="ydeA"
                     /locus_tag="ACIYSB_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGC5428245.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(21761..22426)
                     /gene="marC"
                     /locus_tag="ACIYSB_001163"
     CDS             complement(21761..22426)
                     /gene="marC"
                     /locus_tag="ACIYSB_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGC5428246.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            22638..23072
                     /gene="marR"
                     /locus_tag="ACIYSB_001164"
     CDS             22638..23072
                     /gene="marR"
                     /locus_tag="ACIYSB_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGC5428247.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     GGAAICEQCHQLVGQDLHQELTKNLTADEVATLEYLLKKVLP"
     gene            23092..23475
                     /gene="marA"
                     /locus_tag="ACIYSB_001165"
     CDS             23092..23475
                     /gene="marA"
                     /locus_tag="ACIYSB_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGC5428248.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            23507..23725
                     /gene="marB"
                     /locus_tag="ACIYSB_001166"
     CDS             23507..23725
                     /gene="marB"
                     /locus_tag="ACIYSB_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGC5428249.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSSANVVVVPPSQEH
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(23756..24655)
                     /gene="eamA"
                     /locus_tag="ACIYSB_001167"
     CDS             complement(23756..24655)
                     /gene="eamA"
                     /locus_tag="ACIYSB_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGC5428250.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIM
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            24850..26037
                     /gene="ydeE"
                     /locus_tag="ACIYSB_001168"
     CDS             24850..26037
                     /gene="ydeE"
                     /locus_tag="ACIYSB_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGC5428251.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPEMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            26164..26259
                     /gene="mgtS"
                     /locus_tag="ACIYSB_001169"
     CDS             26164..26259
                     /gene="mgtS"
                     /locus_tag="ACIYSB_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGC5428252.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            26259..26363
                     /gene="mgtT"
                     /locus_tag="ACIYSB_001170"
     CDS             26259..26363
                     /gene="mgtT"
                     /locus_tag="ACIYSB_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGC5428253.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(26478..27368)
                     /gene="dgcZ"
                     /locus_tag="ACIYSB_001171"
     CDS             complement(26478..27368)
                     /gene="dgcZ"
                     /locus_tag="ACIYSB_001171"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGC5428254.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(27623..28015)
                     /gene="ydeI"
                     /locus_tag="ACIYSB_001172"
     CDS             complement(27623..28015)
                     /gene="ydeI"
                     /locus_tag="ACIYSB_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGC5428255.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            28291..28809
                     /gene="ydeJ"
                     /locus_tag="ACIYSB_001173"
     CDS             28291..28809
                     /gene="ydeJ"
                     /locus_tag="ACIYSB_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGC5428256.1"
                     /translation="MNINRDKIVQLVDTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(28853..30898)
                     /gene="dcp"
                     /locus_tag="ACIYSB_001174"
     CDS             complement(28853..30898)
                     /gene="dcp"
                     /locus_tag="ACIYSB_001174"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGC5428257.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQMPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNKTHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPKIMPMLQHRGLNI"
     gene            31035..31781
                     /gene="ydfG"
                     /locus_tag="ACIYSB_001175"
     CDS             31035..31781
                     /gene="ydfG"
                     /locus_tag="ACIYSB_001175"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGC5428258.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            31870..32556
                     /gene="rspR"
                     /locus_tag="ACIYSB_001176"
                     /gene_synonym="ydfH"
     CDS             31870..32556
                     /gene="rspR"
                     /locus_tag="ACIYSB_001176"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGC5428259.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            32733..32936
                     /gene="ydfZ"
                     /locus_tag="ACIYSB_001177"
     CDS             32733..32936
                     /gene="ydfZ"
                     /locus_tag="ACIYSB_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGC5428260.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(32971..34431)
                     /gene="ydfI"
                     /locus_tag="ACIYSB_001178"
     CDS             complement(32971..34431)
                     /gene="ydfI"
                     /locus_tag="ACIYSB_001178"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGC5428261.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADVWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTARVTETYLSLLAHGAKATVAKYSVK"
     gene            complement(34520..35887)
                     /gene="ydfJ"
                     /locus_tag="ACIYSB_001179"
     CDS             complement(34520..35887)
                     /gene="ydfJ"
                     /locus_tag="ACIYSB_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HGC5428262.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            complement(35945..36964)
                     /gene="rspB"
                     /locus_tag="ACIYSB_001180"
     CDS             complement(35945..36964)
                     /gene="rspB"
                     /locus_tag="ACIYSB_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HGC5428263.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQIPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPIVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(36976..38190)
                     /gene="rspA"
                     /locus_tag="ACIYSB_001181"
     CDS             complement(36976..38190)
                     /gene="rspA"
                     /locus_tag="ACIYSB_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HGC5428264.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(38396..38722)
                     /gene="ynfA"
                     /locus_tag="ACIYSB_001182"
     CDS             complement(38396..38722)
                     /gene="ynfA"
                     /locus_tag="ACIYSB_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGC5428265.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            38857..39198
                     /gene="ynfB"
                     /locus_tag="ACIYSB_001183"
     CDS             38857..39198
                     /gene="ynfB"
                     /locus_tag="ACIYSB_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGC5428266.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            39233..39793
                     /gene="speG"
                     /locus_tag="ACIYSB_001184"
     CDS             39233..39793
                     /gene="speG"
                     /locus_tag="ACIYSB_001184"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGC5428267.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(39796..40506)
                     /gene="ynfC"
                     /locus_tag="ACIYSB_001185"
     CDS             complement(39796..40506)
                     /gene="ynfC"
                     /locus_tag="ACIYSB_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGC5428268.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            40614..40919
                     /gene="ynfD"
                     /locus_tag="ACIYSB_001186"
     CDS             40614..40919
                     /gene="ynfD"
                     /locus_tag="ACIYSB_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGC5428269.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSSQDGAPAEPQ"
     gene            41118..43544
                     /gene="ynfE"
                     /locus_tag="ACIYSB_001187"
     CDS             41118..43544
                     /gene="ynfE"
                     /locus_tag="ACIYSB_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HGC5428270.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRNAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAQTRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPEDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            43605..46028
                     /gene="ynfF"
                     /locus_tag="ACIYSB_001188"
     CDS             43605..46028
                     /gene="ynfF"
                     /locus_tag="ACIYSB_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGC5428271.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            46039..46656
                     /gene="ynfG"
                     /locus_tag="ACIYSB_001189"
     CDS             46039..46656
                     /gene="ynfG"
                     /locus_tag="ACIYSB_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGC5428272.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            46658..47512
                     /gene="ynfH"
                     /locus_tag="ACIYSB_001190"
     CDS             46658..47512
                     /gene="ynfH"
                     /locus_tag="ACIYSB_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGC5428273.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGVGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLFSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            47555..48169
                     /gene="dmsD"
                     /locus_tag="ACIYSB_001191"
     CDS             47555..48169
                     /gene="dmsD"
                     /locus_tag="ACIYSB_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGC5428274.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            48328..49620
                     /gene="clcB"
                     /locus_tag="ACIYSB_001192"
     CDS             48328..49620
                     /gene="clcB"
                     /locus_tag="ACIYSB_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGC5428275.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(49573..50268)
                     /gene="bioD"
                     /locus_tag="ACIYSB_001193"
     CDS             complement(49573..50268)
                     /gene="bioD"
                     /locus_tag="ACIYSB_001193"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGC5428276.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(50393..51613)
                     /gene="mlc"
                     /locus_tag="ACIYSB_001194"
                     /gene_synonym="dgsA"
     CDS             complement(50393..51613)
                     /gene="mlc"
                     /locus_tag="ACIYSB_001194"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGC5428277.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(51748..52641)
                     /gene="ynfL"
                     /locus_tag="ACIYSB_001195"
     CDS             complement(51748..52641)
                     /gene="ynfL"
                     /locus_tag="ACIYSB_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5428278.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPESLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            52748..54001
                     /gene="ynfM"
                     /locus_tag="ACIYSB_001196"
     CDS             52748..54001
                     /gene="ynfM"
                     /locus_tag="ACIYSB_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5428279.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(54050..54169)
                     /locus_tag="ACIYSB_001197"
     CDS             complement(54050..54169)
                     /locus_tag="ACIYSB_001197"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGC5428280.1"
                     /translation="MTHSKMHPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            54425..54733
                     /gene="asr"
                     /locus_tag="ACIYSB_001198"
     CDS             54425..54733
                     /gene="asr"
                     /locus_tag="ACIYSB_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGC5428281.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETTTTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            54826..54909
                     /locus_tag="ACIYSB_001199"
     CDS             54826..54909
                     /locus_tag="ACIYSB_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428282.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            55009..55830
                     /gene="ydgD"
                     /locus_tag="ACIYSB_001200"
     CDS             55009..55830
                     /gene="ydgD"
                     /locus_tag="ACIYSB_001200"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGC5428283.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVARSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(55869..56198)
                     /gene="mdtI"
                     /locus_tag="ACIYSB_001201"
     CDS             complement(55869..56198)
                     /gene="mdtI"
                     /locus_tag="ACIYSB_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGC5428284.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(56185..56550)
                     /gene="mdtJ"
                     /locus_tag="ACIYSB_001202"
     CDS             complement(56185..56550)
                     /gene="mdtJ"
                     /locus_tag="ACIYSB_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGC5428285.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            56962..57996
                     /gene="tqsA"
                     /locus_tag="ACIYSB_001203"
     CDS             56962..57996
                     /gene="tqsA"
                     /locus_tag="ACIYSB_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGC5428286.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(58021..59409)
                     /gene="pntB"
                     /locus_tag="ACIYSB_001204"
     CDS             complement(58021..59409)
                     /gene="pntB"
                     /locus_tag="ACIYSB_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGC5428287.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(59420..60952)
                     /gene="pntA"
                     /locus_tag="ACIYSB_001205"
     CDS             complement(59420..60952)
                     /gene="pntA"
                     /locus_tag="ACIYSB_001205"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGC5428288.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            61476..62420
                     /gene="ydgH"
                     /locus_tag="ACIYSB_001206"
     CDS             61476..62420
                     /gene="ydgH"
                     /locus_tag="ACIYSB_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGC5428289.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            62606..63988
                     /gene="ydgI"
                     /locus_tag="ACIYSB_001207"
     CDS             62606..63988
                     /gene="ydgI"
                     /locus_tag="ACIYSB_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGC5428290.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYARDGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     TGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            64025..64747
                     /gene="folM"
                     /locus_tag="ACIYSB_001208"
     CDS             64025..64747
                     /gene="folM"
                     /locus_tag="ACIYSB_001208"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGC5428291.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(64744..65079)
                     /gene="ydgC"
                     /locus_tag="ACIYSB_001209"
     CDS             complement(64744..65079)
                     /gene="ydgC"
                     /locus_tag="ACIYSB_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGC5428292.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            65208..65927
                     /gene="rstA"
                     /locus_tag="ACIYSB_001210"
     CDS             65208..65927
                     /gene="rstA"
                     /locus_tag="ACIYSB_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGC5428293.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            65931..67232
                     /gene="rstB"
                     /locus_tag="ACIYSB_001211"
     CDS             65931..67232
                     /gene="rstB"
                     /locus_tag="ACIYSB_001211"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGC5428294.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEDSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            67308..68237
                     /gene="tus"
                     /locus_tag="ACIYSB_001212"
     CDS             67308..68237
                     /gene="tus"
                     /locus_tag="ACIYSB_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGC5428295.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAIFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(68234..69637)
                     /gene="fumC"
                     /locus_tag="ACIYSB_001213"
     CDS             complement(68234..69637)
                     /gene="fumC"
                     /locus_tag="ACIYSB_001213"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGC5428296.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69780..71426)
                     /gene="fumB"
                     /locus_tag="ACIYSB_001214"
     CDS             complement(69780..71426)
                     /gene="fumB"
                     /locus_tag="ACIYSB_001214"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGC5428297.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            71625..72800
                     /gene="manA"
                     /locus_tag="ACIYSB_001215"
     CDS             71625..72800
                     /gene="manA"
                     /locus_tag="ACIYSB_001215"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGC5428298.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            72901..74409
                     /gene="ydgA"
                     /locus_tag="ACIYSB_001216"
     CDS             72901..74409
                     /gene="ydgA"
                     /locus_tag="ACIYSB_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGC5428299.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(74635..75900)
                     /gene="uidC"
                     /locus_tag="ACIYSB_001217"
     CDS             complement(74635..75900)
                     /gene="uidC"
                     /locus_tag="ACIYSB_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HGC5428300.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(75939..77312)
                     /gene="uidB"
                     /locus_tag="ACIYSB_001218"
     CDS             complement(75939..77312)
                     /gene="uidB"
                     /locus_tag="ACIYSB_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGC5428301.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLN
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(77309..79120)
                     /gene="uidA"
                     /locus_tag="ACIYSB_001219"
     CDS             complement(77309..79120)
                     /gene="uidA"
                     /locus_tag="ACIYSB_001219"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HGC5428302.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(79511..80101)
                     /gene="uidR"
                     /locus_tag="ACIYSB_001220"
     CDS             complement(79511..80101)
                     /gene="uidR"
                     /locus_tag="ACIYSB_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGC5428303.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(80322..>80600)
                     /locus_tag="ACIYSB_001221"
     CDS             complement(80322..>80600)
                     /locus_tag="ACIYSB_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGC5428304.1"
                     /translation="AAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQ
                     KMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT        20424 a        20617 c        19257 g        20302 t
ORIGIN      
        1 ccttaccgga ggcatttcct ccgataaatc catagcccgt tgaattaagg gtggcaccat
       61 tactgacatt tacgataccg ctaccaaaat taccgacatt gatacccaca gtatcgaacg
      121 atgatgtcgc atccatcagg ttcagtgtgc cgataccgct tgctttatcg cccaaaaaag
      181 taatattttg cgatacgaac ttaccgccat cgctaatatt gaggtttccg acgcccgcaa
      241 caccaatggt ggtatagaca ttcttattga cctcccacat tccccccgtg gtgatgctga
      301 cattcccgac gccggtttct ccataaccga gtgaacttcc tccgttgctg acaacaagcc
      361 cctgattact gatattcatt tcgccgtggc cgtcataacc aagattagtt ccgagattag
      421 tgataacgga gttcttatcc ttaacagtaa tgttgcctgt gcctgtttct ttcatccccg
      481 cagtgataat tcccgaatct actttgcctt cactcgagac attaagttca ccgtcgccag
      541 catcaccgat atagatatag cggaaagctt cgcccgttcc gaggaaattc caatgccctt
      601 tatcggtcac gttgacgaca ccgtgggaac cgtcctgaac gcccaccaat gaatattctt
      661 tgttattaat cagtccatta ttggaaatat tgactgtgcc attaccgaaa tatccattat
      721 agagtctgcg taccgtgatg acagagtctt gatcctgcac attcagggtt ccgataccgg
      781 tggcattgcc accgataatc gtattttcag ccgtaaccaa ccccccctcg cgaatagtcg
      841 cctccccagt tccttgatta ccaatttgaa attcaattga ggaatcatta ttttttatta
      901 gccactcgcc acccttttca acgacaacct ggccattact gcccgcctga tagcctaaaa
      961 tggcgacgat tgaactcgtg acgtaaccct tatccgtaat atttaatgaa cctgtgccat
     1021 agctacctat ttcgaataat tcggtcgtca gaacagagtc ctctccctca acattgaccg
     1081 tcccgacgcc tcctgtcgaa gaacctaatc ttaaatagcc tccatcgacg tgacccttct
     1141 gtttaatatt cagcgtcccc gttccggatt gacccacatt taaaggcctt gcattatttc
     1201 cgctatcata caatcgccag gtgccaccca aaacattaac ggtgccctct gagtcctcat
     1261 tagcgccaat gacgctggta gtaatagtgg ttaaggaggc attaccgcca gccaaaatat
     1321 taagcactcc agtgccatac cagccaacca ggtaggcatc ataagcaaca tcagtatcaa
     1381 tattggtaat ctgatcatta tcaacttcca gacttgcacc agacgctgaa ccgcttagtg
     1441 ccagcaaaac acccagcgtc aatctactga atttcgttgt cagtccagag gatttacgca
     1501 aattaaccgt cgatgtttta cctgccctgc gagttaattc cgagcaggcc tgaaatacct
     1561 gtagagtgca attccatatc acgcgataga ttctattcat ataaacgctc catatacaaa
     1621 caatacagcc acgaaagaga tgagtcgtca gacattaaaa atgaaactta ttaaattgtc
     1681 agaggtctgt attgagtgtt agttggaggt gggaaggtgt caatttggct aatttgctaa
     1741 tgctttcgaa ttgatagatt aaaaattgca tagttcttat tttatttaaa tatgaactat
     1801 gagttattgt tgcttaataa gaaaactgtt tctttcaata ggaaaattat catcatcata
     1861 tttaacaaag aatagcacta attgctaaaa atcgaagttt attaaacccc ttttgcatcc
     1921 attagtgtct ttaggaatat tcgctataaa ataagggttg tgcttaatgc tttaagaaaa
     1981 atagcaattt tcccttgaat atagtactgg tgatggaacg atgaatctgc aagctggctt
     2041 ttaacaagcc agctctaaaa gaagggaaat aagaataact atactcaaaa actaacagcc
     2101 acggtcatca tgatgtggct gtcaatgaaa ctataaccca ggcgctttcc ataacgacgt
     2161 cgtcagtcca tgatcaacca gccccagctg atcctgataa actgcctgcc acttatcgcg
     2221 tgaatcctga taaagttcat gcttttccgg gtctggtgtg tgcgtccgtt cccagcgaac
     2281 caggcgttct ccggtttctg ccattgatga aaaaattccg gcaccgacgc cagctgcaat
     2341 ggcacatcct aatgcagtgg cttctttgac caccggaata ttgacgggta atcccgagac
     2401 atcagcgaga atttgactcc ataatttccc ttttgaacct ccgcctgcaa agactaacga
     2461 tgaaggatga atattcgaga aatcagcaat ttgctgcaag ttacacgctg atacaatcgc
     2521 cgcattttct tccagcgcac ggaacaatgt cgctttgtta catttatccg ggtcaatgga
     2581 caagttaata aaggaaggcg cagcgtgata ccaggtttta aagcgcattc tgtcggagaa
     2641 gatcggcatt acgccccacg acccaggcgg cacccgactg gccatctctt ccagcagcgt
     2701 ataggtgtcg atgcctaaac gttcggcaat cagtttttct tcggcacaga aagcatcgcg
     2761 gaaccagcgc atggtgagtc cggtaaaaaa gcttatagat tcagcttgta ccatgccagg
     2821 aataacatga ggattaacgc gcacgttcat ttctgggtct gtcaccggcg cggctaaatt
     2881 tacaacttgc tgccagaatg tgccgccaag aaccgcggtt tgtgccggac gcacaacgcc
     2941 taacccaagg caaccaagct gcacgtcgcc tcctccaacg accaccggag tgcccgcctt
     3001 cagaccgcag agttccgccg cttgtgaact taccacgccc agcaatgtgc cggtttcttt
     3061 gacaggagaa agaatatcgg cacgtaggcc agccatatcc agcaatgcag gtttccagtc
     3121 acgggtggtt agatcaagaa gtcccgtggt gccagcgtta gagggatcca ccgccagttc
     3181 gccgctgagc atataggcca gccagtcgct gatcatggtg atggttgatg cctgacggta
     3241 aatatcggaa cgatggtgcg ccagccaaag taatctgggg atggcactta aagccagtgt
     3301 ttgtccggtc gcgcgataaa cttcgttttc aaaggtattg ttgtgcagtt ctttaagttc
     3361 gctaacttcg cgtgccgctc tggcatccac attggcgcag gcccagatcg gggctccttc
     3421 attattatat aaaacaatgc cttcacgcat cgaacatgcc gaaacggcag cgatatactc
     3481 cggggctatg ccggcgttgt gcagcgcctg gcgcatacac tcacacgcca gttgccagtt
     3541 tttgttgaga tcaaattcca tagaaccagg aacgtccggt actgccagat gccgccactc
     3601 cgcctgtccc actgctattt gattgccttc caggtcgaat atcacagccc gaatacttcc
     3661 ggtgcctgca tccagcgcca tcaggtagta ctttgattct gaaagggtaa agagtcgagc
     3721 cattagatta tcctcggctt atgcgttctg tctgttcctc tatacgttct ccatcattcc
     3781 cggtaataag gtcatgcaaa tttaactacg taaaatcgcc gctgctgtgt cctgatcggt
     3841 aaccagtgcg ttgatataac cgcctttcat tgcagcggca attgcttcgg ctttattttc
     3901 tccccctgcc acgccaaccc ggacgggtat ggtcttcagc gcgcttaaag gtaagccaat
     3961 cagttcgtta tgtattttga tattcgtgac aacgtcacct tttgcatcaa aaaagtagcc
     4021 taaaatgtcg ccaaccgccc cttttcggcc aatcattaac tgttcgccct ggctgatata
     4081 accggagcga atgattgtcg catcgtcctg ttgactcaca gcaccaatgc cgacaatcgc
     4141 cacatccgct gcttgcgcgg ctaacagaac atctttgacg caattttcat tttttagcgt
     4201 acgggcaatg tcagcggagg atgcccgcaa cggagccgga ataatattca cactgcacgc
     4261 cgcgttaagc tgcccgattc ccgtcatata agaaccgacg ccaccggaga gcgtgaccag
     4321 gcgaatttgc tgtgacgaaa taaaaccact taagcgttgc agcgtattca tggttgcctc
     4381 gccaaaacca atcgccagca tctgttgtgg ttgaagtaaa ctcatcaaca tatgcgccgc
     4441 gcctatcccc agtcgcccac cgacatcagc atccgcaagc ccagggatca cccggacatg
     4501 ttgcagcgaa aactgacgac gtaattgagt ttcatattcc agacagcctt caaagcgaga
     4561 attaatctgt acgcgaataa tgccggactg atgccctttc tccagcaatc gcgacacttt
     4621 caaacgtgtc aggccgagac gatcgctgat ctcgctctgg gtcagcccgt cgtgatagta
     4681 aaaccacgcg atccgcgcga cctgttcttc ttcacacatt ccctgttctg aaattgccga
     4741 atcgttgatt gtcataattc attcttcact ttgaacatat ttaaatcttt aatgcaattg
     4801 ttcagttctt gctcatttat atctgtgatg gcaaccacag tttgactcta cgagcatgaa
     4861 caaacgcaac cgtgaaaatc aaaatagcat aaattgtgat ctattcgtcg gaaatatgtg
     4921 caatgtccac ctaaggttat gaacaaatta aaagcagaaa tacatttgtt caaaactcac
     4981 ctgcaaaact gaacggggga aatatgcaaa cgagtgatac ccgcgcgtta ccgctacttt
     5041 gcgcccgctc ggtttataaa cagtattcag gggtcaatgt cctgaaaggc atcgatttta
     5101 cgttgcatca gggggaggtc cacgccctgc tcggcggcaa tggtgccggt aaatcgacgt
     5161 taatgaagat tattgccggt attacccctg ctgatagcgg tacgctggag attgagggca
     5221 acaactacgt cagattaacg ccagttcatg ctcatcagct gggtatttat ctcgttcccc
     5281 aggaaccgct gcttttccca agcctgtcga taaaagaaaa catcctgttt gggctggcaa
     5341 aaaaacagct ctccatgcag aaaatgaaga acttgctggc ggcgctgggc tgccagtttg
     5401 atctgcatag tctggcagga tcgctggatg tcgccgatcg ccaaatggtg gaaatcctcc
     5461 gcgggctgat gcgcgactcg cggattctga tcctcgatga acctaccgcc tcgcttaccc
     5521 ctgcggaaac cgaacgcttg tttagtcgct tgcaagagct gcttgctact ggcgtgggta
     5581 ttgtttttat ctcgcataag ctgccggaaa ttcgccagat tgccgatcga attagcgtga
     5641 tgcgcgacgg aaccatcgcc ttaagcggca aaaccagcga actgtctacc gacgacatta
     5701 ttcaggccat caccccagcg gtacgggaaa aatcgctctc tgccagccaa aaattatggc
     5761 tggagttacc tggtaaccgc ccacaacatg ccgccggaac gccggtgctg acactggaaa
     5821 atctgaccgg cgaaggtttc aggaatgtca gcctgacgct caatgccgga gaaattctgg
     5881 gcctggctgg gctggtgggg gccggacgca cagaactggc cgagacgctc tatggtctgc
     5941 gtactttgcg tggcggacgc attatgctga atggtaaaga gatcaataaa ttatccactg
     6001 gagaacgttt actgcgcggt ctggtttatc tgccggaaga tcgccagtca tccggactga
     6061 atctcgatgc ttcgctggcc tggaacgtct gcgcccttac tcataacctt cgtggattct
     6121 gggcgaaaac cgcgaaagat aatgccaccc tggaacgtta tcgtcgggcg ctgaatatta
     6181 aattcaacca accggaacaa gctgcacgga cattatccgg tggcaaccag caaaaaatcc
     6241 tcattgccaa atgcctggaa gcttcgccgc aagtattgat tgtcgatgag ccgacgcgcg
     6301 gcgtggatgt ctcggcccgt aatgatatct accagctgtt gcgcagcatc gccgcacaaa
     6361 atgtggctgt gctgcttatc tcctccgacc tggaagagat cgaactgatg gcagatcgtg
     6421 tgtatgtgat gcatcagggc gaaattaccc actctgcact gaccgagcgc gatattaatg
     6481 tcgagactat tatgcgcgtt gccttcggcg atagtcagcg tcaggaggcg tcatgctgaa
     6541 gtttattcag aacaaccgtg aaatcacggc actgctggcg gtggtgctgc tgtttgtatt
     6601 acccggtttt ctcgaccgcc agtatttaag tgtgcaaacg ctgaccatgg tttatagcag
     6661 cgcgcaaatc ctgatcctgc tggcaatggg cgcgacgctg gtaatgctta cgcgcaatat
     6721 tgatgtttca gtgggttcga ttaccggaat gtgcgcggtg ctgttgggga tgttactgaa
     6781 cgcaggatat tcactacctg ttgcttgtgt cgcgacttta ctgcttggtt tgctcgcggg
     6841 atttttcaac ggtgtcctgg tcgcgtggct aaagatccct gccattgttg ccacccttgg
     6901 cacgttaggg ttgtacagag gcatcatgtt gctgtggact ggcggcaaat ggattgaagg
     6961 gttacccgcc gaactgaaac agctctccgc cccgctgctg cttggcgttt cagcaattgg
     7021 ttggttgacg ataattctgg tggcatttat ggcctggctg ctggcaaaga cggcgtttgg
     7081 acgcagtttt tatgccacgg gcgataattt acagggcgct cgtcaactgg gcgttcgtac
     7141 tgaagccatt cgcattgtgg cattttcgtt gaacggctgc atggcggcac tggcgggaat
     7201 tgtgtttgct tcgcagattg gttttatccc caaccagacc ggtaccgggc tggagatgaa
     7261 agcaattgca gcctgcgtgc tgggcggcat tagtttgctc ggtggttccg gtgcgatcat
     7321 tggtgcggta ctcggcgcat ggttcctgac gcagatcgat agcgtactgg tgctgttgcg
     7381 cattccggca tggtggaatg attttatcgc gggtctggtt ctgctggcgg tgctggtgtt
     7441 tgatggacgc ctgcgttgtg cgctggaacg taatctacgg cggcaaaaat atgcccgctt
     7501 tatgacgcca ccgccatccg ttaaacccgc ttcgtcaggt aaaaaacggg aggccgcata
     7561 atgcgtattc gctacggttg ggaactggct cttgccgcac tgctcgttat tgagattgtc
     7621 gcatttggtg caattaaccc gcgaatgtta gatctcaata tgttgctgtt cagcaccagt
     7681 gactttatct gcattggcat tgtcgcccta ccgctaacga tggtgattgt cagtggcggg
     7741 atcgatattt cgtttggttc gaccatcggc ctctgcgcca ttgcattggg cgtactgttt
     7801 caaagtggtg tgccgatgcc gctggcgata ctcctgacct tactgctcgg cgcattgtgc
     7861 gggctgatca acgccggatt aattatctat accaaagtta acccgctggt gattacgctt
     7921 ggcacgctgt atctgtttgc cggaagcgct ctgctgcttt ccggtatggc cggagcgacg
     7981 gggtacgaag gtattggtgg attcccgatg gcgtttacag atttcgctaa cctggatgtg
     8041 ctgggactcc ccgttccgct gattatcttc ctgatatgtc tcctcgtttt ctggctctgg
     8101 ctgcataaaa cccatgccgg acgtaatgtg tttttgattg ggcaaagccc gcgcgtggcg
     8161 ctttatagcg cgattccagt taaccgtacc ttatgtgcgc tctatgccat gacggggctg
     8221 gcgtctgcgg tcgccgctgt gctgctggta tcgtattttg gttcagcacg ttccgatctc
     8281 ggtgcgtcgt ttctgatgcc cgccatcacc gccgtggtgc ttggcggggc caatatttat
     8341 ggtggttccg gttccattat cggcaccgcc attgcggttt tattagtggg atatttgcaa
     8401 caaggtttgc aaatggcagg agtgccaaat caggtgtcca gcgccctttc cggtgcgcta
     8461 cttatcgtcg ttgtcgtagg tcgttccgtt agcctgcatc gccagcaaat taaagagtgg
     8521 ctggcgcgtc gggccaataa cccattgcca taaaggatat cttcatgaca cttcatcgct
     8581 ttaagaaaat cgccttactt agcgctcttg gcattgccgc aatctctatg aatgtgcagg
     8641 ccgcagagcg tattgcattt attcccaaac tggttggcgt gggatttttt accagcggtg
     8701 gcaacggcgc acaacaagcg ggtaaagagc tgggcgttga tgtgacctac gacgggccga
     8761 cagaacccag tgtttctggt caggtacagt tgattaataa cttcgtcaat caaggttata
     8821 acgccattat cgtttctgcg gtttcgcctg atggcttgtg tccggcactg aaacgcgcca
     8881 tgcaacgtgg tgtgagagtg ctgacctggg actctgatac taaaccggag tgccgctctt
     8941 actacattaa tcagggaacg cccgcccagt taggaggtat gttggtggat atggcggcgc
     9001 gtcaggtgaa taaagacaaa gccaaagtcg cgtttttcta ctcaagcccc accgttacgg
     9061 accaaaacca gtgggtgaaa gaagcgaaag cgaaaatcgc caaagagcat ccaggctggg
     9121 aaattgtcac tacgcagttt ggctataacg atgccactaa atcgttacaa accgcagaag
     9181 gaatattaaa agcgtatagc gatctcgacg ccattatcgc ccccgatgcc aacgccctgc
     9241 ccgctgccgc acaagccgca gaaaacttga aaaatgacaa agtagcgatt gtcggattca
     9301 gtacgccaaa tgtgatgcgc ccgtatgtag agcgcggcac ggtgaaagaa cttggcctgt
     9361 gggatgtggt tcagcaaggc aaaatttcag tgtatgtcgc ggatgcatta ttgaaaaaag
     9421 gatcaatgaa aacgggcgac aagctggata tcaagggcgt aggtcaggtt gaagtctcgc
     9481 caaacagcgt tcagggctat gactacgaag cggatgataa tggcatcgta ctgttaccgg
     9541 agcgcgtgat attcaacaaa gagaatatcg gcaaatacga tttctgatgt gcattactta
     9601 accggagtaa gttatggcag atttagacga tattaaagat ggtaaagatt ttcgtaccga
     9661 tcaaccgcaa aaaaatatcc cttttaccct gaaaggttgc ggtgcgctgg attggggaat
     9721 gcagtcacgc ttatcgcgga tatttaatcc gaaaacgggt aaaaccgtga tgctggcttt
     9781 tgaccatggt tattttcagg gaccgactac cggacttgaa cgcattgata taaatatcgc
     9841 cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc gcagcgtagt
     9901 tccccctgcg accaataggc cggtggtact gcgggcgtca ggtgcgaact ctattctggc
     9961 ggaattaagt aatgaagccg tggcgttatc gatggatgac gccgtgcgcc tgaacagttg
    10021 cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga tcaaaaatat
    10081 tattcagctg gttgatgccg gaatgaaagt gggaatgccg accatggccg tgactggcgt
    10141 gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa tcgccgctga
    10201 aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac ggattgttgc
    10261 cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc gcgaggcgct
    10321 ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg ggcgtaatat
    10381 tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg ttcaccataa
    10441 cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac tgcggatcta
    10501 aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga caaggttgac
    10561 gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga aggcaatttg
    10621 cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatatcta cgaagcctat
    10681 aaagatgaag acgcagtggc gttccataaa accacgcccc actacaaaac ctgtgtcgcg
    10741 aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg tttgatgccg
    10801 tgaggcgaat ttatcaattt tatctacaat tggggtaacg cgctgacggg agtaaaaaaa
    10861 tgtctgactg gaacccctct ttatatctac acttttccgc tgaacgatcg cgtccggcgg
    10921 tggagctgct tgccagagtg ccgctggaaa atgtcgaata tgttgccgat cttggctgtg
    10981 gcccaggtaa cagcaccgcc cttctacaac aacgttggcc tgcggccagg ataacaggca
    11041 tcgactcgtc tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg
    11101 tggaagcaga catccgcaac tggcaaccgg tacaggcact cgatctgatt tttgctaatg
    11161 cctcactgca atggctgccc gaccactacg aattgtttcc tcatctggtt tctttactta
    11221 atccgcaggg cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc
    11281 tcatgcgcga agttgcctgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg
    11341 gcgttcatgc ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa
    11401 ccacctacta tcaccagatg ccgtcacacc aggcgattat cgattgggtg actgccactg
    11461 gattacgtcc gtggttacag gatctgaccg agagcgaaca gcagcttttt cttaagcgct
    11521 accatcagat gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat
    11581 ttccgcgtct gtttattgtt gcccggcgta tggagtaaat atcacgtcag ctggtaatga
    11641 cgatcgggaa gaatttttgc tggaatttcg gcttcatcgt tcatctgtaa caggtcaatt
    11701 tcaatagcgt tgcagatggc atccagtggt aaatcattgt tttcagtacc gaacggatct
    11761 tccagttctt ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata
    11821 aaaggcgtca tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga
    11881 taaacggtac gatgcaaaat cagggtgtag gcaaagggaa ttggcgtata ggcaatgcgc
    11941 tcgcatcctg ccaggaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg
    12001 atatctgaaa gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag
    12061 atacggttag ctggagagtt cgaagccagt acacgctgaa gatcttcagt cttgagataa
    12121 tgagccagca cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaaa
    12181 gcgatttgca gccgggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc
    12241 tcgcgcagta acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg
    12301 taacgggcgt acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga
    12361 atgctgaacg gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaaa
    12421 ataacagcga tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact
    12481 gagccgtgcc agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt
    12541 atcttcaggc gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc
    12601 tacttacaaa ttacctacaa gcttacaaca ttagcaggcg ctgcatgtgg cacttgttaa
    12661 tgataaaggc atataggatg ttgtaactaa tatggtgaaa taagaaaccc ggtcgaaacc
    12721 gggttcagaa gtaacggtgt tattagcaca acggacgtac agcttcgcgc atcccttttt
    12781 cgagaatcgc atccaggtca ttagcaacct gctcgaccag accaggcact tgcgtcaggt
    12841 cctgctccca gtggtctttt tctgccaaaa caatcgctac cagttcctgg gtgccgataa
    12901 cacggtcacg atgctggctc cagagttgct gataacgttc cagccagtgt gcatcgtcct
    12961 gtaccggata tgtttcgccg ttacgctcac cacgatagaa cgcaatcaat gccgctaatg
    13021 cgaaagtaag gcgcgccgga agtgtgccgt ttgccttctg ccctgccagc agctgtggca
    13081 ggatgcgggt gcggaacttg gtcataccgt tgagcgcgat agacagcagc tgatgcttaa
    13141 tgtacgggtt acggaaacgc ccggtgactg cactggcgaa agattccagt tcatcacgag
    13201 gcaaatccag taccggaata atttcttcgt agatagcttt ttcaacgaat gcgcaaattt
    13261 cagcatcgtt catcgcttca cctacggtat ccagccctgc ctggaacgcc accggcacca
    13321 gcgcggtgtg cgcaccgttg aggattgcca ctttgcgttc tttgtacggt ttaatatcgt
    13381 caacaatcag cacgttgagc ggatatttgt ccagacgcag ttcagtcgct aaggatttcg
    13441 gtccctgaat cacaaacagg taaaagtgtt cagcggtgtc gagaaaacca tcgtgataac
    13501 ccagttcttc ttccagttta gccacttcat cgcgcggata accggtaacg atacggtcca
    13561 ccagcgtaga acagaagctg ttagcctgat ccagccattg aataaatgct tctggcagtg
    13621 cccactcttg cgcatagcgc agcaccagtt cacgcaacgc gtcgccattg tagtcaatca
    13681 actcacacgg aatgatgatc caacctttat ccagcgcacc gttgaaatgg ctaaagcgtt
    13741 cgaacagcag acgggtcagt tttgccggat agcttaccgc tggcgcgtca tcgaatttat
    13801 cgcccgcatg gtagctgata cccgcttcgg tggtgttaga gaaaacaaag cgcatttccg
    13861 ggttgtgcgc cagtttcagg aattcatcgt attcactgta gacgctgatt tcacgattaa
    13921 ccgagcgaat cagacgcgcg tcgctgaccg cttccccctt ctcattcagg ccacggataa
    13981 tggtggtgta cagaccatcc tgcgtgctca gtgacggcgg gaatgaagtt tcaatcggac
    14041 gaacaacgac cacgccagaa ttcagatcgg tgtgctcatt caggagatcg atttgccagt
    14101 caacaaaggc gcgcaggaag ttaccttcac caaactgaat gatacgttct ggatactgtg
    14161 caccgggaaa atcgcgacga tttagtgttt tcacaatggg ttcccttctg attagtcata
    14221 caacctgttt gaattggtac gacaggttag caaactttaa tacgccgaac ccctgttttg
    14281 atcaactcct gatgattaat gagcagtttt atgagaaaag tgtggcgcgg atcatggttt
    14341 aatcgaggaa aaacgccttt tcctggatca taaagtggta gaacacattg cattcaaatc
    14401 gcgcgtaatg aataaagatg tcagacaact tcctcaccgt aacgcatagt gctggtacgg
    14461 ttgcgcccat ctttcttcga gcgatacaga cacgtatcgg cttcgaccat caatttatta
    14521 aagtcatcgg tcagcgttag gtacgatgcg cgaccactac cgacgccaat acttaccgtg
    14581 agataaagcg ttttttgttg ccaggtgaat ggttgcagtt caacgccttt acggattttt
    14641 tccgccatta gcagaccatc gacaggattc accgacggca ctgcaacagc aaattcttcg
    14701 ccgcccattc gcgccaccag ccccttatca ccgacaatct tctgaatatg ccgggcaaac
    14761 acgcttaaca ctttatcgcc acattcatgt ccatagttat cgttaatgct tttgaaatag
    14821 tcgatatcaa gcagcatgac agtcagatgt tgtgtctgtt tcagcgatgg acttttcagc
    14881 gcctcataaa gaccggaccg tgagtaaacc tgagtcagaa aatcaaagtc ggctcgcagc
    14941 gcaacttgct tcattagcga attgatcgct gccacgctaa aagaaaccat aattgggcat
    15001 atcgccatcg tggcaatacc gagacgtgcg gagaacattt gcggaatgga gaacggcgaa
    15061 ccgaccgaga tatcaatcac cgaatttgcc accagtacga tttccaccgc accggtgaca
    15121 aaggtcaaca gacatgttac ctgcggcgta tagcgcactg cacaccagat taatgcaggc
    15181 agcgggaacg ccagactccc cgctccgcca atgactaccg aggcaataac cgacacaata
    15241 agcgcgatgg cgggcatcat ctgttctgct ttaaagcgcg gcagcactcc aggaatagcc
    15301 aacgtcagca tacaaggcac gatcaacacg cccgttgaga attgctcact gaaccagtcc
    15361 gcaagcaaag gccagaaatc cagactatca atactgaccg aaccaagata gcatgttcgc
    15421 tgacagcggc ggcaaataaa ggtaccctgg gaatgctatt ttgtcgtttt ccgcaattct
    15481 catggctgat tcatctgtac aaccagccag tgatagatta ccgccacgat ataacgttgt
    15541 tgttcttcgc ttaacgaaac accctgcgga atgttatgcc acttagccag acaaccgcga
    15601 cagcaggtcg cggtggcgtg ctgagcgata aacaccggat gcccgcgcat gggggtttgc
    15661 ttaccgtcat tagccggtaa tgccggagca agacgtttag cgacaaaatc cgccgcatgt
    15721 tgttcgatga ctggcgcgcc tttctccagg caatactgac gctctttcat gcccagacga
    15781 aagcgagagc gaaattttga acgggataaa cgcgcgaaga gtggatcgag agactgcatt
    15841 aataaaccga acgccctaac tgtttcgtca attgttcaag gacggcaata cccgcaagcg
    15901 agttacctgc atcatccagt tccggactcc agacagcgat ggccatttca tgcggaacaa
    15961 tcgccacaat accgccacca acgccagatt tcgccggtag ccccacccgc caggcaaact
    16021 cccccgcgtt ctggtacata ccactggtcg ccatcagcgc gttaatttgc cgcgcctgca
    16081 ttggcgtcac cactggttca tcaatatgaa tagctttccc ctgattagcc agaaagacaa
    16141 acgtccgggc cagctctaca cagctcattt tcagagcgca gtaatgaaag tagttttgca
    16201 gaacggttgt cacgtcatga tggaaattgc caaacgactt catcagccag gcgatagccg
    16261 catttcgcgc ggaatgttca aattcggaac gcgctaccac cgtatcgtag gaaatatcag
    16321 acacaccgct taagccgcgc acgacttcca gcatacgttg ccgtggtgcg cttaatcgcc
    16381 cttgcaacat atcgcagacc accagcgcac cggcattaat gaacggatta cgcggtatac
    16441 cctgctccat ttccagttgc actaaggaat tgaacggtga tccagacgga tctttgccga
    16501 cgcgttgcca gatttcctct tcggagtaat gacgcatggc gacaacgaga ctcagcactt
    16561 tggaaataga ctgaatggaa aaacgttctt gcgcgtctcc ggcctgaaaa agctgtccgt
    16621 caacggtaca gatagcaatc cccaatcggg aaccgtctac tgtagccagc gccggaatat
    16681 aatccgcgac tttaccctga ccaatgagcg gccgcacttg ccgcaagatg ttttctaaaa
    16741 ttgcattatc catggcgact gccactttct actcctggac cgcaggtctg aaaagacctg
    16801 cgagtatatc agagctgaat atgtcgcgtc agatccggtc tttccacacc gtctggatat
    16861 tacagaattc gtgtaagccg aaatgggaaa gctcacgacc aaagccactc tttttcacgc
    16921 caccaaaggc cactcgcgcg tcgctggcac aataaccatt gataaacacc ccaccgcatt
    16981 ccagacgtgc cgccatctgt ctggcctgtg tttcgtcagt ggtaaaaatg gtcgctgaaa
    17041 ggccgaactc actatcatta gccagttcca gtgcatgttc tgcatctttc gcaatggtga
    17101 ttgccgcaac ggggccaaac atttcttccc gaaacgcggt catttctggg gtaacattcg
    17161 ccagaaccgt tggcggatag tagttacctg ccccagccat cttttccccg cccagtaaca
    17221 aacgcgcacc ctgcgccagg gttttctcca cctgatgatg cagctcatca cgtaaatcaa
    17281 aacgagccat tggtccgaga gcgttctctt cgtcacgggg atcgcccatt ttcaaggctg
    17341 ccgcagctgc cacaaaacgt tcggtaaatg ccgaagcaat tccctcttcg ataataaagc
    17401 gttttgccgc tgcacatacc tgtccggtat tctgataacg tccggctacc gccgctttca
    17461 ccgccagttc cagatcggca tcgttaagca caataaacgg atccgaaccg cccagttcca
    17521 gtacgcattt tttcagtgcc gctccagcct gtgcgccaat agccgctccc gcacgaacac
    17581 ttccggtcac cgtgacagca gcaatgcgcg agtctttaat catctgactg acaccgtcgt
    17641 tgtcggcatt cagccagcca tatacgcctt gtgggatacc cgcatcttta aacacctggg
    17701 caatgagctg tgcacagccc atcacattcg gcgcatgttt aagtaagtag ccgttacctg
    17761 caagaatgat gggaacagcg ccacgcatca cctgccataa cggaaaattc cacggcataa
    17821 tcgccagaat cgtccccaac ggtcgatact caataaccgc ctgctgattt tccaccagcg
    17881 taggttccgc cttcagcatt gccggaccat gttctgcata ccagtcacac aaattcgccg
    17941 atttcgccac ttcagcgcgc gcctggttga ttggtttgcc catttcgcgg gtgatcattt
    18001 gcgccatttc ttcgctacga gcgcgcagag ccttaccgat atcacgcagt ttttcagcac
    18061 gataatctat atttgtctcg cgccagtcgc gaaagcctgc tgccgccagc tgaagtgcgt
    18121 tttcgatatc gtcagcgcca gcccacggca gcacagaaag ttgttcaccc gtggcaggat
    18181 ttatcgaaat tgcatgagtt gccggagtaa tggtcatcgg ggtatctcct ttatgagtca
    18241 tggtatgaag atacgcagat ttactcttgc tttaaaatga ataatattaa gccacttatt
    18301 cacgaatcga gaatgctatg gatctgaccc aactggagat gttcaacgcg gttgccgaag
    18361 ctggcagcat aacccaggct gcagcaaaag tgcatcgcgt gccgtcgaat ctcactaccc
    18421 gtctgcgcca gctggaaaca gaactggggg ttgatctgtt tattcgcgag aatcagcgtt
    18481 tacgtctctc tcctgccggg cataactttt tacgctatag ccaacaaatt ctcgcgttag
    18541 tggatgaagc gcggagcgtt gtcgctggcg atgagccgca aggtttattt tctcttggtt
    18601 cgctggaaag caccgctgca gtgcgcattc cagccacgct ggcggaattc aaccgtcgtt
    18661 atcccaaaat tcagttttca ctttccaccg gcccttccgg cactatgctg gatggtgtac
    18721 tggagggaaa actgaatgcg gcgtttattg atggacccat taaccatact gccatcgacg
    18781 ggataccggt ataccgcgag gaactgatga tcgtcacgcc acaaggatat gcgccagtaa
    18841 cccgtgccag tcaggttaat ggcagtaaca tttatgcctt ccgcgccaat tgttcgtatc
    18901 gtcgccactt cgagagctgg tttcatgctg acggtgccgc tccgggaact atccatgaga
    18961 tggagtctta tcacggaatg ttggcctgtg tgatcgcagg agcaggcatt gcgcttattc
    19021 cgcgctctat gctggaaagt atgccggggc atcaccaggt tgaagcgtgg ccgttagctg
    19081 agcaatggcg ttggttaaca acctggctgg tctggcgtcg tggtgcgaaa acacgtccgc
    19141 tcgaggcatt tattcaactg actgctggat gtgcctgact cggcaagaca gggatatcaa
    19201 tgagctattt ttgatagttc ttgcggttaa tatgctctat atagtgatgt tccgatgact
    19261 tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac atatctcttc
    19321 ctgatgattt tcccgtacgt aatattgcac cgcaggtaaa agaagtttta aaggatttta
    19381 ttgatgcact tagtacaata atatgtaatg aagagtggcg cacgtcttta aacatcaact
    19441 cagccacaaa aaagatattt aataaccttg acaacttatc atatattcag aggacttctt
    19501 ttcggggtaa cgacacgcta tacaatgaaa aggttcagtt taaactcact tatcccgcca
    19561 ggaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta agccctattt
    19621 atttagataa ttttcgccat gatggagaaa ttaatatttt ttgcgctccc aacccaaagc
    19681 ctgtcactat ggggcgcgta tttcagaccg gcgtcgagcg tgtactcttt ctgtttctga
    19741 atgattttat tgaacaattt ccaatgatca accctggtgt tcccatcaaa agagcgcata
    19801 caccacatat tgaacccctg ccttcggatc accataccgc tgcagattac ttacgccagt
    19861 ttgatttgct tgtcctgaat tttatctctc gcggtaattt tgtcatactc ccccgattat
    19921 ggaataactc tgaggttcac agatggttcg tcaataaaga tcctaacttg atcaccgcca
    19981 ttctcgacat aacggacagc gaattaaaag aggatttgtt gcaaagcctg atggattcat
    20041 taggttctaa caaacatgta ctacccgaag tctgcatctg ctttttatcc cttttagcag
    20101 aacaagaatc tcctcatttt cagaacttgt ttttattttt cgccaatatg ttattgcact
    20161 atcaccaatt tatgaatccc aatgaaagtg atttgaatga cgtgttgatg ccagcatcat
    20221 taagtgatga taaaattatc aaacatatgg cgcgcaggac cctcaaactg tttgtaaaaa
    20281 atgaaacacc gccaaaagtt actcacgaag acctggtgaa aaacaggcct cgctcccctg
    20341 tcagaccacc tatacccgca accgccaaaa cgccagacct ccctgaacgt cattaaaccg
    20401 tgatgttacc gactctctga cgcgtgaaag aatcagcgtc agagaaacgg aaaacgcgat
    20461 ccagatcaca aatgcattgt attcacatca ttaaccgttt taagatcatt tcatcacttt
    20521 ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca aagtggcgtg
    20581 gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg aatttgtccc
    20641 tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc aggtcggcat
    20701 catgttgacc atttacgcat gggtagtagc gctaatgtca ttgcctttta tgttaatgac
    20761 cagtcaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta ttgccagcca
    20821 cgtactgtcg tttttgtcgt ggagctttac cgttctggtg atcagtcgca ttggtgtggc
    20881 ttttgcacat gcgattttct ggtcgattac ggcgtctctg gcgatccgta tggctccggc
    20941 cgggaagcga gcacaggcat tgagtttaat tgccaccggt acagcactgg cgatggtctt
    21001 aggtttacct ctcgggcgca ttgtgggcca gtatttcggt tggcgaatga ccttcttcgc
    21061 gattggtatt ggggcgctta tcaccctttt gtgcctgatt aagttacttc ccttactgcc
    21121 cagtgagcat tccggttcac tgaaaagcct cccgctattg ttccgccgcc cggcattgat
    21181 gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat acagctatat
    21241 cgagcctttt gtacaaaaca ttgcgggatt cagcgccaac tttgccacgg cattactgtt
    21301 attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg gtaatcagta
    21361 tgcgtctgcg ttggtgagta cggcgattgc gctgttgctg gtgtgcctgg cattgctgtt
    21421 acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg ggatcgcgat
    21481 gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag atgctaccga
    21541 cgtcgcgatg gcgctattct ccggcatatt taatattgga atcggggcgg gtgcgttggt
    21601 aggtaatcag gtgagtttgc actggtcaat gtcgatgatt ggttatgtgg gcgcggtgcc
    21661 tgcttttgcc gcgttaattt ggtcaatcat tatatttcgc cgctggccag tgacactcga
    21721 agaacagacg caatagttga aaggcccatt cgggcctttt ttaatggtac gttttaatga
    21781 tttccaggat gccgttaata ataaactgca cacccataca taccagcagg aatcccatca
    21841 gacgggagat cgcttcaatg ccacccttgc ccaccagccg cataattgcg ccggagctgc
    21901 gtaggcttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc gcaaccatca
    21961 gtacccaatc agcgaaggtt gaactctgac gcactgtgga cgccgagcta ataatcatcg
    22021 ctatggttcc cggaccggca gtacttggca ttgccagcgg cacaaaggca atattggcac
    22081 tgggttcatc ttccagctct tccgacttgc ttttcgcctc cggtgaatca atcgctttct
    22141 gttgcggaaa gagcatccga aaaccgataa acgcgacgat taagccgcct gcaattcgca
    22201 gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccggcgtaa tacgccacca
    22261 tcatgatggc aaatacgtac accgaggcca tcaacgactg acgattacgt tcggcactgt
    22321 tcatgttgcc tgccaggcca agaaataacg cgacagttgt taatgggtta gctaacggca
    22381 gcaacaccac cagccccagg ccaattgctt taaacaaatc taacattggt ggttgttatc
    22441 ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caaggataaa gtgtcactct
    22501 ttagctagcc ttgcatcgca ttgaacaaaa cttgaaccga tttagcaaaa cgtggcatcg
    22561 gtcaattcat tcatttgact tatacttgcc tgggcaatat tatcccctgc aactaattac
    22621 ttgccagggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt attccattgg
    22681 gtcgcttaat ccatatggtt aatcagaaga aagatcgcct gcttaacgag tatctgtctc
    22741 cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc gcggcgtgta
    22801 ttactccggt tgaactgaaa aaggtattgt cggtcgacct gggagcactg acccgtatgc
    22861 tggatcgcct ggtctgtaaa ggctgggtgg aaaggttgcc gaacccgaat gacaagcgcg
    22921 gcgtactggt aaaacttacc accggcggcg cggcaatatg tgaacaatgc catcaattag
    22981 ttggccagga cctgcaccaa gaattaacaa aaaacctgac ggcggacgaa gtggcaacac
    23041 ttgagtattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac gatgtccaga
    23101 cgcaatactg acgctattac cattcatagc attttggact ggatcgagga caacctggaa
    23161 tcgccactgt cactggagaa agtgtcagag cgttcgggtt actccaaatg gcacctgcaa
    23221 cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag ccgtaagatg
    23281 acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct ggcagaacga
    23341 tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt tgatgttccg
    23401 ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca tccattaaat
    23461 cattacaaca gctagttgaa aacgtgacaa cgtcactgag gcaatcatga aaccactttc
    23521 atccgcaata gcagctgcgc ttattctctt ttccgcgcag ggcgttgcgg aacaaaccac
    23581 gcagccagtt gttacttctt ctgccaatgt cgtggttgtt cccccatcgc aggaacaccc
    23641 accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc tcggcgtgcc
    23701 ctattataat caacacgcta tgtagtttgt tctggccccg acatctcggg gcttattaac
    23761 ttcccacctt taccgcttta cgccaccgca agccaaatac attgatatac agcccggtca
    23821 taatgagcac cgcacctaaa aattgcagac ccgttaagcg ttcatccaac aatagtgccg
    23881 cacttgccag tcctactacg ggcaccagta acgataacgg tgcaacccgc caggtttcat
    23941 agcgtcccag taacgtcccc cagatcccat aaccaacaat tgtcgccaca aacgccagat
    24001 acatcagaga caagatggtg gtcatatcga tagtaaccag actgtgaatc atggttgcgg
    24061 aaccatcgag aatcagcgag gcaacaaaga agggaatgat tgggattaaa gcgctccaga
    24121 ttaccagcga catcaccgcc ggacgcgttg agtgcgacat gatcttttta ttgaagatgt
    24181 tgccacacgc ccaactaaat gctgccgcca gggtcaacat aaagccgagc atcgccacat
    24241 gctgaccgtt cagactatct tcgattaaca ccagtacgcc aaaaatcgct aaggcgatcc
    24301 ccgccaattg tttgccatgc agtcgctccc cgaaagtaaa cgcaccaagc atgatagtaa
    24361 aaaacgcctg tgcctgtaac accagcgaag ccagtccagc aggcataccg aagttaatgg
    24421 cacaaaaaag aaaagcaaac tgcgcaaaac tgatggttaa tccatacccc agcagcaaat
    24481 tcagtggtac tttcggtcgt gcgacaaaaa agatagccgg aaaagcgacc agcataaagc
    24541 gcaaaccggc cagcatcagc ggtggcatgt tatgaagccc cactttgatg accacaaaat
    24601 ttagccccca tacgaccact accagtagcg ccaacacccc atcttttcgc gacattctac
    24661 cgcctctgaa tttcatcttt tgtaagcaat caacttagct gaatttactt ttctttaaca
    24721 gttgattcgt tagtcgccgg ttacgacggc attaatgcgc aaataagtcg ctatacttcg
    24781 gatttttgcc atgctatttc tttacatctc taaaacaaaa cataacgaaa cgcactgccg
    24841 gacagacaaa tgaacttatc cctacgacgc tctaccagcg cccttcttgc ctcgtcgttg
    24901 ttattaacca tcggacgcgg cgctacgctg ccatttatga ccatttactt gagtcgccag
    24961 tacagcctga gtgtcgatct aatcggttat gcgatgacaa ttgcgctcac tattggcgtc
    25021 gtttttagcc tcggttttgg tatcctggcg gataagttcg acaagaaacg ctatatgtta
    25081 ctggcaatta ccgccttcgc cagcggtttt attgccattc ctttagtgaa taacgtgacg
    25141 ctggttgtgc tcttttttgc cctcattaac tgcgcctatt ctgtttttgc taccgtgctg
    25201 aaagcctggt ttgccgacaa tctttcgtcc accagcaaaa cgaaaatctt ctcaatcaac
    25261 tacaccatgc taaacattgg ctggaccatc ggtccgccgc tcggcacgct gttggtgatg
    25321 cagagcatca atctgccctt ctggctggca gctatctgtt ccgcgtttcc catgcttttc
    25381 attcaaattt gggtaaagcg cagcgagaaa atcatcgcca cggaaacagg cagtgtctgg
    25441 tcgccgaaag ttttattaca agataaagca ctgttgtggt ttacctgctc tggttttctg
    25501 gcttcttttg taagcggcgc atttgcttca tgcatttcac aatatgtgat ggtgattgct
    25561 gatggggatt ttgccgaaaa ggtggtcgcg gttgttcttc cggtgaatgc tgccatggtg
    25621 gttacgttgc aatattccgt gggccgccga cttaacccgg ctaacatccg cgcgctgatg
    25681 acagcaggca ccctctgttt cgtcatcggt ctggtcggtt ttattttttc cggcaacagc
    25741 ctgctattgt ggggtatgtc agctgcggta tttactgtcg gtgaaatcat ttatgcgccg
    25801 ggcgagtata tgttgattga ccatattgcg ccgccagaaa tgaaagccag ctatttttcc
    25861 gcccagtctt taggctggct tggtgccgcg attaacccat tagtgagtgg cgtagtgcta
    25921 accagcctgc cgccttcctc gctgtttgtc atcttagcgt tggtgatcat tgctgcgtgg
    25981 gtgctgatgt taaaagggat tcgagcaaga ccgtgggggc agcccgcgct ttgttgattt
    26041 aagtcgaaca caataaagat ttaattcagc cttcgtttag gttacctctg ctaatatctt
    26101 tctcattgag atgaaaatta aggtaagcga ggaaacacac cacaccataa acggaggcaa
    26161 ataatgctgg gtaatatgaa tgtttttatg gccgtactgg gaataatttt attttctggt
    26221 tttctggccg cgtatttcag ccacaaatgg gatgactaat gaacggagat aatccctcac
    26281 ctaaccggcc ccttgttaca gttgtgtaca aggggcctga tttttatgac ggcgaaaaaa
    26341 aaccgccagt aaaccggcgg tgaatgcttg catggataga tttgtgtttt gcttttacgc
    26401 taacaggcat tttcctgcac tgataacgaa tcgttgacac agtagcatca gttttctcaa
    26461 tgaatgttaa acggagctta aactcggtta atcacatttt gttcgtcaat aaacatgcag
    26521 cgatttcttc cggtttgctt accctcatac attgcccggt ccgctcttcc aatgaccaca
    26581 tccagaggct cttcaggaaa tgcgcgactc acacctgctg tcacggtaat gttgatatgc
    26641 ccttcagaat gtgtgatggc atggttatcg actaactggc aaattctgac acctgcacga
    26701 catgcttctt catcattagc cgctttgaca ataatgataa attcttcgcc cccgtagcga
    26761 taaaccgttt cgtaatcacg cgtccaactg gctaagtaag ttgccagggt gcgtaatact
    26821 acatcgccga ttaaatgccc gtaggtatca ttaaccaatt taaatcggtc aatatccaac
    26881 aacattaaat aaagattcag aggctcagcg ttgcgtaact gatgatcaaa ggattcatca
    26941 agaacccgac gacccggcaa tcccgtcaaa acatccatat tgctacggat cgtcagcaaa
    27001 taaattttgt aatcggttaa tgccgcagta aaagaaagca acccctcctg aaaggcgtcg
    27061 aaatgcgcgt cctgccagtg attttcaaca atagccagca ttaattcccg accacagtta
    27121 tgcatatgtt gatgggcaga atccattagc cgaacgtaag gtaattcatc gttatcgagt
    27181 ggccccagat gatcaatcca ccgaccaaac tggcacagtc cataagaatg gttatccgtt
    27241 atttctggct tactggcatc tctcgcgacc acgctgtgaa acatactcac cagccactgg
    27301 tagtgggcat cgatagcctt attgagattt aacaagatgg catcaatttc cgttgtcttc
    27361 ttgatcattg ccactccttt ttcacagttc cttgtgcgcg ctattctaac gagagaaaag
    27421 caaaattacg tcaatatttt catagaaatc cgaagttatg agtcatctct gagataacat
    27481 tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa ttctgtaaat tacctctgca
    27541 ttatcgtaaa taaaaggatg acaaatagca taacccaata ccctaatggc ccagtagttc
    27601 aggccatcag gctaatttat ttttatttct gcaaatgagt gacccgaacg acggccggcg
    27661 cgcttttctt atccagactg ccactaatgt tgatcatctg gtccggctga acttctcgtc
    27721 catcaaagac ggccgcagga ataacgacat taatttcacc gctcttatcg cgaaaaacgt
    27781 aacggtcctc tcctttgtga gaaatcaaat taccgcgtag tgaaaccgaa gcgccatcgt
    27841 gcatggtttt tgcgaaatca acggtcattt tttttgcatc atcggttccg cgatagccat
    27901 cttctattgc atgaggcggc ggtggcgctg catcctgttt taaaccgccc tggtcatctg
    27961 ccaacgcata aggcatgaca agaaaacttg ctaatacaat ggcctgaaat ttcatactaa
    28021 ctccttaatt gcgtttggtt tgacttatta agtctggttg ctatttttat aattgccaaa
    28081 taagaatatt gccaattgtt ataaggcatt taaaatcagc caactagctg tcaaatatac
    28141 agagaattta actcactaaa gttaagaaga ttgaaaagtc ttaaacatat tttcagaata
    28201 atcggattta tatgtttgaa aattattata ttggacgagc atacagaaaa agcaaatcac
    28261 ctttacatat aaaagcgtgg acaaaaaaca gtgaacatta atagagataa aattgtacaa
    28321 cttgtagata ccgatactat tgaaaacctg acatccgcgt tgagtcaaag acttatcgcg
    28381 gatcaattac gcttaactac cgccgaatca tgcaccggcg gtaagttggc tagcgccctg
    28441 tgtgcagctg aagatacacc caaattttac ggtgcaggct ttgttacttt caccgatcag
    28501 gcaaagatga aaatcctcag cgtaagccag caatctcttg aacgatattc tgcggtgagt
    28561 gagaaagtgg cagcagaaat ggcaaccggt gccatagagc gtgcggatgc tgatgtcagt
    28621 attgccatta ccggctacgg cggaccggag ggcggtgaag atggtacgcc agcgggtacc
    28681 gtctggtttg cgtggcatat taaaggccag aactacactg cggttatgca ttttgctggc
    28741 gactgcgaaa cggtattagc tttagcggtg aggtttgccc tcgcccagct gctgcaatta
    28801 ctgctataac caggctggcc tggcgatatc tcaggccagc cattggtggt gtttatatgt
    28861 tcaagccacg atgttgcagc atcggcataa tcttaggtgc cttaccgcgc cattgtcgat
    28921 acaggcgttc cagatcttcg ctgttacctc tggaaaggat cgcctcgcga aaacgcagcc
    28981 cattttcacg cgttaatccg ccctgctcaa caaaccactg ataaccatca tcggccaaca
    29041 tttgcgtcca cagataagcg taataacctg cagcatatcc gccaccaaaa atatgggcga
    29101 aataactgct gcgatagcgt ggcggtatag caggaagatc catattttcc gccaccagcg
    29161 cccgcaattc aaaatcatcg acatcctgca ttgcttcgtt ttcttccagg caatgccagc
    29221 gcatatcgag aagtgcggcg ctaagcagtt cgctcatctc ataccctttg ttgaacaggc
    29281 tggcattacg cattttctgt tgcagttcgt caggcattgc tgccccgctc tgataatgcc
    29341 gggcgtagcg agcgaatacc tgcggatgcg ttgcccagtg ttcgttgatt tgcgacggaa
    29401 attcgacaaa atcacgcggc gtgttggtgc cggaaagcgt ggcataacgc tggcgggcaa
    29461 aaaggccgtg cagcgtatga ccaaattcat ggaataaggt tatgacatca tcccagagta
    29521 acaacgcagg ctcaccggca gcgggtttct gataattgca gacgttataa attaccggat
    29581 gtgttttatt aagcgttgat tgctcaacaa aattgcccat ccatgcaccg ccgctttttg
    29641 aatcacgggc gaagaaatca ccgtaaaata acgccagccc cacgccatta tgatcaaaaa
    29701 tttcccacac acgaacgtca ggatggtaga caggaatatc aaaacgttcg acaaacttaa
    29761 taccgaagag ctgattcgcg gtccagaata caccttcatt taacaccgtg tttaattcaa
    29821 aatatggctt gagctgcgcc tcatcaagat caaatttctc ccgccgtacc tgttccgcat
    29881 aaaatgccca gtcccacggc tgcgcgctaa acccgccctg ctgcttatcg ataaccgcct
    29941 gtatggaggc taattcatcg ctcgcacgtt gacgcgccgc tggaacaatt tcccgcataa
    30001 agttaagtgc tgcttcaggt gtttttgcca tctgatcggc gattttccat gcggcataat
    30061 gaggaaaacc aagtagtgtt gcctgttgtg cacggatctc caccagacgt tgaatgatag
    30121 cgcgggtatc attggcatca tttttttccg ctcgcgtcca gcccgcaata aacagttttt
    30181 cacgcgtcgc acgatcgcgc atttcggcaa gcgccggttg ttgggtggta ttcagcagcg
    30241 gaatcagcca tttgttatcc agacctttct cgcgagccgc ctctgccgcc agcgcaatct
    30301 cttgctcact cattcctgcc agctgcgcga tatcgttcac aaccagaccg ccggatttat
    30361 ttgctgccag taatcgctgg ttaaactggc tggtcagggt cgcagcttct gtattcagta
    30421 cttttaattt tgctttatca gcttgcgcaa gtttggctcc ggcaaggaca aaacgttgat
    30481 gaatcacctc caccaggcgg atggattcac tatcaagccc cagggattca cggcgctgcc
    30541 agacagcatc tacccgcgcg aataattcac cgttcagata gatatcatta gccagttccg
    30601 ccagttcagc ggaaaactgc tcgtcaagac gctgtaattc atcattggta tgcgccgcag
    30661 tcatcgcaaa aaagacgctg gtaacgcggg taagtaattc tccgctttgt tccagtgcca
    30721 gaatagtatt gttgaaatca ggcatttgcg ggttaagcgc gatggcagca atttctgccc
    30781 gcttttgctg cattccctca tcgaatgccg ggcgatagtg atgattggca atttgatcaa
    30841 aatggggagc cagatacggc agtgtgcttt gcacaaggaa aggattcatt gttgtcattt
    30901 tcttctcctg aacgcgaggt gttccatagc gtaggcttac tgataacgga gtgcaatctt
    30961 gcaatccagt attacccgct cttaagcatc ccgtgctatg ttattgacac acaaaagcgt
    31021 tgaggaacag tgagatgatc gttttagtaa ctggagcaac ggcaggtttt ggtgaatgca
    31081 ttactcgtcg ttttattcaa caagggcata aagttatcgc cactggccgt cgccaggaac
    31141 ggttgcagga gttaaaagac gaactgggag ataatctgta tatcgcccaa ctggacgttc
    31201 gcaaccgcgc cgctattgaa gagatgctgg catcgcttcc tgccgagtgg tgcaatattg
    31261 atatcctggt aaataatgcc ggcctggcgt tgggcatgga gcctgcgcat aaagccagcg
    31321 ttgaagactg ggaaacgatg attgatacca acaacaaagg cctggtatat atgacgcgcg
    31381 ccgtcttacc gggtatggtt gaacgtaatc atggtcatat tattaacatt ggctcaacgg
    31441 caggtagctg gccgtatgcc ggtggtaacg tttacggtgc gacgaaagcg tttgttcgtc
    31501 agtttagcct gaatctgcgt acggatctgc atggtacggc ggtgcgcgtc accgacatcg
    31561 aaccgggtct ggtgggtggt accgagtttt ccaatgtccg ctttaaaggc gatgacggta
    31621 aagcagaaaa aacctatcaa aataccgttg cattgacgcc agaagatgtc agcgaagccg
    31681 tctggtgggt gtcaacgctg cctgctcacg tcaatatcaa taccctggaa atgatgccgg
    31741 ttacccaaag ctatgccgga ctgaatgtcc accgtcagta atttttatac ccggcgtaac
    31801 tgccgggtta ttgcttgtca caaaaaagtg gtagactcat gcagttaact cactcacaag
    31861 caagaacgaa tgaccgtcga aacgcaactt aatcccacac agcctgtcaa tcagcagatt
    31921 tatcgtattc ttcgtcgcga cattgtccat tgcctgattg ctccaggcac accgttgtcg
    31981 gaaaaagaag tttctgttcg tttcaatgtg tcacgccagc cggttcgtga agcctttatt
    32041 aaactggcgg aaaacggcct gattcaaatt cgtccgcaac gtggcagcta cgtcaacaaa
    32101 atttccatgg cccaggtgcg caacggcagt tttatccgtc aggccattga gtgcgcggtg
    32161 gcgcgtcggg cggcgagcat gattaccgaa agccagtgct atcaactgga acaaaatctt
    32221 caccagcaac gcattgccat tgagcgcaag caactggatg atttttttga acttgatgac
    32281 aacttccatc aactcctgac gcagattgcc gactgtcaac tggcgtggga taccattgag
    32341 aacctgaaag cgaccgttga tcgcgtgcgc tatatgagtt tcgaccacgt ttctccacca
    32401 gaaatgctgt tacgccagca tcttgatatt ttctctgccc tgcaaaaacg tgatggcgat
    32461 gcggtagaac gtgcaatgac gcaacatttg caggaaatca gcgaatccgt gcgccagatc
    32521 cgccaggaaa acagcgactg gtttagcgaa gagtaattca tttcctctca tcccatccgg
    32581 ggtgagagtc ttttcccccg ccttatggct catgcatgca tcaaaaaaga tgtgagcttg
    32641 atcaaaaaca aaaaatattt cactcgacag gagtatttat attgcgcccg ttacgtgggc
    32701 ttcgactgta aatcagaaag gagaaaacac ctatgacgac ctacgatcgt aaccgtaacg
    32761 caatcaccac tggcagccgt gttatggtta gcggcaccgg tcacactggc aagatcctgt
    32821 cgattgatac tgaaggtctg accgctgagc aaatccgccg cggaaaaacc gtagttgttg
    32881 aaggttgtga agagaaactg gcaccactgg acctgattcg tctcggcatg aactaagcgt
    32941 gtgaatgccg ccgatggcgg cattgctttt ttacttcacg gaatattttg ccacggtcgc
    33001 tttcgcgcca tgcgctaata aagacaagta cgtttccgtc actcttgcag taaacaaact
    33061 attgtctggc aaatcatcac caaagatcgc cttaatcgcc agcaatgact ggacgcgcgc
    33121 tttcccttcg gcactacttt gtacagcctt ctgaataaca ggtaacagtg ggtcactgat
    33181 ttctatcgga tttccctgtt catcaacacc accgacataa cgcatccaac ccgcgacgcc
    33241 cagcgccagc agatcgaact tgctgtcatg cgccagatgc cagcgaacag aatccaacat
    33301 ccgctgtggc aatttctggc taccatccat cgcaatctgc caggttcgat gacgtaacgc
    33361 cgggttgcta tagcgtgcaa ttaatcggtt agcgtaatct tgcaaatcaa cgccctgcac
    33421 tttcaacgtc ggcgcttgtt cctgcaacat caagccatac gccgcataac gataatgttc
    33481 atcttccata cagtcattaa tgtgctgata tcctgcaaga taccccagat acgccaggaa
    33541 tgaatgactg ccgttgagca tgcgcaactt catctcttca taaggcagca catcgctaac
    33601 cagttcggct cccgcttttt cccattccgg acgtccggca acaaagttat cttctattac
    33661 ccactggcgg aaaggttcac aggcaacgcc cgcaggatcg cgcacaccgg taagttgttc
    33721 gattttcgcc agcgtatcct ctgtcactgc gggcacaata cggtccacca ttgttgatgg
    33781 gaaagtcacg ttatcttcga tccattgtgc cagttttaca tcaacggctt gtgcgtagga
    33841 agtgacaacg tcacgcataa catgaccgtt ttctggcatg ttgtcacatg acatgacggt
    33901 aaatgcggga agtcctgccg ctttacggcg agccagcgcc tcaacaatca cccctgttgc
    33961 tgttttcggc tggtggggat tttgcacgtc ggcagctacc atcgggtgat caagcattaa
    34021 ctgtccggtc gccggagagt ggaaataccc tttttcggtg attgtcagag agacaatcgc
    34081 gatttgcggt tcacacatcg ctgccaacac ggtttctaag ccatctatct gtacgtgcaa
    34141 ggctttttta acgacgccaa cgacgcgagc cgtccacaca tcggccgaca tttccgcaac
    34201 ggtataaaga ttatcttgct gttgtaaatc ggcaatttgc tgttcgccgc cgattaagtt
    34261 gacctcataa tatccccagt cactgaaatg ttccgtagca agaatatcgg catacacacc
    34321 ctgatgcgca cggtgaaatg caccaaagcc taaatgaaca attcttggag ccaggttatt
    34381 acgatcataa acagggagtg tcgcttttgc tgataacaaa ttatttccca taacaattcc
    34441 ttaaatataa atatggcaag ctatatgttt tgttatatga ataaaaatcc cctctccggt
    34501 aagagaaggg attaagggtt tacagacttc tggaaggttg cgcagctctt acaacacgcg
    34561 gttgatcttc cgcagcgtct tccagcgcac ttaaatcacg gtctttcacc tctggcattt
    34621 tcagcgcaga gattaaacca atcactgaat atgccatgat cataatggcg atcggatacc
    34681 aggattccgt catggtgcag aaaatacccg ccaggatagg accaaaaccg gaagcgataa
    34741 gaccaccaat ttctttagaa atagccatcc gggtaaagcg gtttttacag ccgaacattt
    34801 ctgccatggt aatgttttcc agagcaaata atcccagcac cgcacagtta tgaatcacaa
    34861 tcagtgcaac cataatggtg ctcggggcat agcttttatc tacaatgata gaaagcattg
    34921 gccatgccag cacaatcgcg gaggtattca taataatata cgggatccgg cgaccaattt
    34981 tatcggataa ccaaccaagg aacggaatgg tcataaagcc gagaatcgaa ctgatcatca
    35041 atgcatctgt tggaattgct ttgttaaaca ataacgtctg cactaaatag cctgcaagga
    35101 aagtctgaat taacccggag ttacccgcct gaccaaaacg cagccctgtt gccagccaga
    35161 aggatttgct ctggaacatg ctaccagcag gtgcaggttt tgctgtcggt tggttactgt
    35221 cgttaacctt ctcaaagacc gggctttctt tcagattcat acgcaaccag atagcaaaga
    35281 ccatcacgac aacgctcgcc aggaacggta tacgccatcc ccacgccagc agttcctctt
    35341 tactgagaat gaagaacata aaggcccaga ttgccgttgc gctcaaggtt ccgcagttag
    35401 ttcccatagc cacaaatgag gagataattc cgcgcttacc ttttggcgca tattccgcca
    35461 gcatcgtacc ggcaccggaa atttccgcac ctgcacccaa cccctgaata atacgcaacg
    35521 tcaccagcaa gatgggtgca aaaacaccaa tctgtgcata ggtcggtaac acaccaatta
    35581 aggtggtaca gatccccatc atggtgatgg taataaagag cactttttta cgccctattc
    35641 tgtcgcccat tttgccgaaa ataaatgctc cgacaatacg cgccacataa cctgcaccgt
    35701 aggttcccat tgccagaatt aacgccattg ccgttgatga ttcaggaaaa aatatttcat
    35761 gaaacactaa cgctgcgccg agcgaatata actggaaatc cataaattca agcgcagtgc
    35821 ccagccatcc cgatactgct gctttcacca aatccttagt gcttctttcg tgtttttcta
    35881 ttgtcataat ggttatctct aaaaaagagg taagatgcgt actacttact cgccgttatt
    35941 ggtattattc agaaaaagtg agtaagactt tgcagcaatg tttttgatcc tgttcaaata
    36001 aactaatggc atcagcaaca tgctggaaat caaacgtatg ggtaattaat ttttctggtt
    36061 taattaaccc tttacttaac cagtcgataa cgatcgggaa tttatttgca tttaagcgtg
    36121 aagagaaaat agagagttct tttccggtaa ttccttgctg aatcacttca gacggttcac
    36181 tggagaaccc catcaataca atacgtgccg ctggagaagc cagcgttacg gcctctttca
    36241 ggatagaagg atgacaagcc gcatcgataa ttaatgtcgg cttgatgcct ttttcagtga
    36301 aaatctcgcc aagcggtatc tggctgttat taatcgccca gtcagccccg ctctctttcg
    36361 ctttttccag tcgttcatca atgcgatcgg caacaatcac atttttaacg ttatagacgc
    36421 cttttaatac ctgaacgatc gtcaggccga ttggaccggc accataaacc agaacggtat
    36481 cattttcagt cggttgacca tgtccggtta cgttagccgc aatggtaaaa ggttcgatca
    36541 ttaccgcata ttgatcggcc actgcttcag gaattttcca cgcatttttt gccggaacca
    36601 cggcatattc actgaaacca ccgtcagcgt gcacacctaa tacagccagt gtcgtacaaa
    36661 cgttcggttt acctatagag cacggatagc aatgcccaca gctgaccacc ggatcgacag
    36721 caacacgttc accgactctg gcgctttcca cgccttcacc cactgcatca atgacgccaa
    36781 agaattcatg accaatgacg cgcggatatt tcgcaaaagg attatgccca cgataaatat
    36841 ggctatctga accacaaatt ccggcaagtt tcacttttac tcgtacttca cccgctgacg
    36901 gggtgggtat ttcacgttcg acaatcgcca gttgattcgg tttttcaatt aatatgcttt
    36961 tcattatctt actccttacc agttccacag cgtgccatct tccagacgtg cgactggtag
    37021 ataagcaggt tcatagggat atttcgccgc cagcttttca tcgaattcga taccaagacc
    37081 cggtttgtct cccggatgca tatagccgtt atcgaaagtc cagttgtgcg ggaagacttc
    37141 gagcatttgt tcggaataac ccatgtattc ctggacaccg aaattgggga cccacagatc
    37201 aaagtgcagc gccgcagcca tgcagactgg tgacaaatcg gaaggaccgt gtgagccagt
    37261 acgtacctga tacagcgaag caaaatcggc aatccggcgc ataccggtaa ttccgcctgc
    37321 atgggtcagc gtggtgcgga tataatcgat gagttgctct tcaatcagtt gtttgcagtc
    37381 ccagatgctg ttgaagactt cacccactgc gatgggtgtg acggtatgtt ggcgaatgag
    37441 acggaagcat tcctggtttt ccgcaggcgt cgggtcttcc atccagaaca tgcgataatc
    37501 ttcaatgctt ttaccaaagc gcgccgcttc aataggcgtt aagcgatggt gcatgtcatg
    37561 cagcaaatgt tcattaaaac caaacttgtt acgtaccgcg tcaaacaatt tcggcatgaa
    37621 atcgaggtat ttctccgtcg accacagctg ctcttccggc cactgtcctt tggttgcggg
    37681 ttcataagcc agacctttac ctttcgacat gccgtaggtg gttttcatac cagggattcc
    37741 gcactgcacg cggatggctt tgaatccaag ctcttgatga cgggcataat catccagagc
    37801 ttcatcaata ctgtgaccgg tggtatggca ataaaccatc accccttcac gagacgcgcc
    37861 gccgagtaac tggtaaagcg gcatgttggc agctttggct ttaatatccc acagcgccat
    37921 atcaaccgct gaaatggccg acatcgtaac cggaccgcga cgccagtaag cacctttata
    37981 gaaaaactgc cagatatctt cgatacggtg cgcatcgcga ccaataagct gcggacaaag
    38041 gtgatcctgc aaataagagg ccacggaaag ctcacgtcca ttgagggtgg catccccaag
    38101 gcccgtaata ccgtcctcag tggtgatttt taatgtgacg aaattacgcc ccggacaggt
    38161 aacaaaaact tcagccttta cgatcttcat gttcgattcc ttgcatcgct tgtcgtgatg
    38221 catgaaatct acgcaactga gctactacca tacaagtata aagatcgaaa aaagccggag
    38281 tgatcacaaa aaaaggcgta tatttgcgct gtgaatggtt gacaaaagat gaaatagaat
    38341 accttttgtc agctgacact tcctcttatc ttattgataa aatggattta tgttcctacg
    38401 tgcgccccca gcccgcaaca atgatcaaca tgccgcaaag cgcaatcaac gcacccgtcc
    38461 agtcataaag agtcagtttc acgccatcca caacgcgcag ccacatcaac gccgtgcaga
    38521 cataaacgcc accataagcc gcgtaaacac gcccactcgc cgctggatgc aacgttaaca
    38581 accagacaaa cagcgccagt gaaatccccg ccggaagcaa cagccagata ctggcgtttc
    38641 gttttaacca caaccagggc agaaagcatc caataatttc acacagcgca gtagcaaaaa
    38701 atagtaacgt tgttttaatc atctttgtct cttattgaca tcatgtatag ttatagggcg
    38761 acataatatc atcaatataa acaccctcct ggtacgtttt gcgtccgcag tggatggtgt
    38821 agaatcacct ttaatcattc atacagggaa tgaattatga aaatcactct cagcaaacga
    38881 atcggcctgc tcgctattct gctgccttgc gcactggcat tgagcacaac tgttcatgcc
    38941 gaaactaaca aactggtgat tgagtctggc gacagtgcac aaagccgcca gcacgccgct
    39001 atggaaaaag agcaatggaa tgacacgcgc aatctgcgcc agaaagtgaa taaacgcact
    39061 gaaaaagagt gggataaagc cgacgccgct tttgataacc gcgataaatg tgagcaaagc
    39121 gccaacatca atgcctactg ggagcccaat actttgcgct gcctggaccg tcgaactggc
    39181 cgcgttatta ccccctaacc tgttattgat ttaaggaatg taaggacacg ttatgccaag
    39241 cgcccacagt gttaagctac gcccgctgga gcgtgaagat ttacgctatg tacatcaact
    39301 cgacaataac gccagtgtga tgcgttactg gtttgaggaa ccctacgaag cctttgttga
    39361 actctctgat ctgtatgata agcatattca cgatcagagc gaacggcgct ttgtggtgga
    39421 atgtgacggc gaaaaagccg gtctggtgga gctggtggaa attaaccatg ttcatcgccg
    39481 cgcagaattt cagataatta tctccccgga gtatcagggg aaaggtctgg caacccgtgc
    39541 cgccaaatta gcaatggact atggctttac cgttctcaat ctctataagc tgtatctgat
    39601 cgttgataaa gagaatgaaa aagcgattca catttaccgc aagcttggct tttcggttga
    39661 aggtgaattg atgcacgagt tctttattaa tggtcaatat cgtaatgcca ttcgcatgtg
    39721 tatattccag catcagtatc tggcagagca caaaacaccg ggtcagactc tcctgaagcc
    39781 gaccgcacaa tagcattaat aataatcgat cgtatttttg atggtgtaaa cccgttcgac
    39841 ggcgggtttt actccttcat caacaatgat tagctgacag tccaccggat tagcgtgact
    39901 gtcatattca cagctctgtt ttacattacc aacccgttga ttattcagta aagtaaccgc
    39961 tgtgtaatct aattttttga tcggatccgt tgatggcgtg gcgctgacag ataatgtttt
    40021 gtcgttactt ttcgtggttt tacccagcgg ataaccctga tcatcatagc gatattccat
    40081 ctgcatttgt ttgctgctgg ctttaatcac gaagccatta tcatcggttt cccaactcac
    40141 cccggcagaa ggtaattctg ctagctggca ttttccctgt aaacgtactc tcttctccag
    40201 cgtctcggca tcacggtaat aattggcgtc cagtaccaga gcgaccacgg tattattttc
    40261 cagatccagt aattcgagtg aatcaaaaca gccttcttcc gacaaagtcc cagaaacacg
    40321 tttcgtcact tcaccttgct catccattaa tgtctgagtg aaatctttta ccggaccacg
    40381 cagcggatca aaatcgaatt cattagagaa actggccatc tcaggggtaa atgaaagtgt
    40441 tacctctgtg cggtcacatc ctgtgaggaa tatcgcgagt aagcatggta gtaatttgta
    40501 tttcacaaca gtcaccagag agtagagatg attctcaatc atagtagcaa atacagtact
    40561 ttacacgtta aatgctatgc ttaaagaagt tatcttcgcg taaggagctt atgatgaaac
    40621 tctcaacctg ctgtgccgcg cttctgctcg ccctcgcctc acccgcggta ctcgccgcgc
    40681 cgggatcctg tgagcgcata cagagcgata tatcacagcg cattatcaat aatggcgtac
    40741 ccgaaagcag cttcacgtta agtattgtac ccaatgacca ggttgatcag cctgattccc
    40801 aggtcgtcgg ccattgcgct aatgatacgc ataaaattct ctatacccgc accaccagcg
    40861 gtaacgtctc tgctcccgcg cagtctagcc aggatggcgc gcctgccgaa ccgcagtaac
    40921 acattatcga ctgaacgccg gatatgacaa atccggcgat ttgaacatac aacataatcc
    40981 caccttatta ctcataccct tctattgata tggattaata attcttaacc caaaatgggt
    41041 agactccctc tattgttagc gcgctaaata ttcaatatat aaacttttat ataacgataa
    41101 agaacaggga gtgagttatg tccaaaaatg aacgaatggt gggcatcagc cgcagaacac
    41161 tcgttaaatc taccgcgata ggttctctgg cgctggctgc aggcggtttt tctttgccgt
    41221 ttaccctgcg caatgcagca gcagcggtac aacaggcccg cgaaaaagtg gtctggggtg
    41281 cctgttccgt caactgtggt agccgctgtg cacttcgtct acatgttaaa gataatgaag
    41341 tgacctgggt ggaaactgac aataccggca gcgatgagta cggcaaccat caggtacgcg
    41401 cctgtttgcg cggtcgctcc atccgccggc gtattaatca tcccgatcgc ttgaattacc
    41461 caatgaaacg cgtgggcaaa cgcggcgaag gcaaattcga acggattagc tgggatgaag
    41521 ccctggatac tatcgccagt agcctgaaga aaaccgtcga acaatatggc aatgaggctg
    41581 tatatattca gtactcttcg gggatcgttg gcggcaatat gacccgctct tcgccatcag
    41641 cctcggcggt caaacgcctg atgaactgct acggcggttc actcaaccag tatggctcct
    41701 acagcactgc gcaaatttcc tgtgccatgc cctacaccta cggcagtaat gatggcaaca
    41761 gcaccacgga tattgaaaac agcaagctgg tcgtgatgtt tggtaacaac ccggcacaaa
    41821 cccgcatgag cggtggtggc atcacttatc ttcttgaaaa agcgcgcgag aaatcgaacg
    41881 ccaaaatgat tgttatcgat ccgcgatata ccgatacggc tgccggtcgt gaagacgaat
    41941 ggctccctat tcgcccgggc accgatgccg cgctggttgc gggtattgcc tgggtattga
    42001 ttaacgaaaa tctcgttgat caaccttttc tcgataaata ctgcgtcggc tatgacgaaa
    42061 aaaccttacc cgcagatgca cccaaaaatg gtcactataa agcctatatt cttggtgaag
    42121 gtgacgataa aacagcgaaa acgccgcagt gggcttcgca aattaccggt atcccggagg
    42181 accgtatcat caaactggcg cgtgaaattg gcacagcaaa acccgcttat atctgccagg
    42241 gctgggggcc acaacgccag gcaaacggcg aactgactgc acgcgctatt gctatgttac
    42301 ctattttgac gggcaatgtc ggcatcagcg gcggaaatag tggcgcgcgt gaatcgacct
    42361 ataccattac catagaacgc ctgccggtgc tggataatcc ggtcaaaacg tcaatctcct
    42421 gcttcagctg gacagatgct atcgatcatg gtccgcaaat gacggcaatc cgcgacggcg
    42481 tccgcggcaa agataaactg gatgtgccca ttaagttcat ctggaactac gcgggaaata
    42541 ccctcgttaa tcagcattct gacatcaaca aaacgcatga aattctgcag gacgaatcga
    42601 aatgcgaaat gattgtggtc atcgaaaact ttatgacctc atcggcaaaa tatgccgaca
    42661 ttctgctgcc agacctgatg accgttgagc aggaagatat tattcctaac gactacgccg
    42721 gtaacatggg atatctcatt ttcctccagc ctgtcaccag cgaaaaattc gaacgcaaac
    42781 cgatttactg gatcctgagt gaagtcgcga aacgtcttgg acctgacgtc tatcaaaagt
    42841 tcacagaagg tcgcacgcag gaacaatggt tacaacatct gtacgccaaa atgcttgcca
    42901 aagatccggc gttaccgtct tacgacgaac tgaaaaaaat gggtatctat aagcgtaaag
    42961 atcccaatgg ccattttgtc gcctacaaag catttcgtga cgaccccgag gcaaatccac
    43021 ttaaaacgcc ttccggtaag attgaaattt attccagcag gctggcggaa attgcccgta
    43081 cctgggaact ggaaaaagat gaagtgataa gcccattgcc agtttatgcc tcaacctttg
    43141 aaggctggaa ctcccctgaa cgtagaacct tcccactgca actgtttggt ttccattaca
    43201 aatcccgtac tcactcgacc tacggcaata ttgatctcct gaaggctgcc tgccgtcagg
    43261 aggtgtggat caaccctata gatgcgcaga aacgtgggat tgccaacggc gatatggtgc
    43321 gggtgtttaa ccatcgtggc gaagttcggc taccagccaa agtaacacca cgtattctcc
    43381 ctggagttag cgctatgggc cagggagcct ggcacgaggc caatatgtct ggcgataaaa
    43441 tcgaccatgg cggctgtgtg aatacgctaa ccactctgcg cccttcacca ctggcgaagg
    43501 gaaacccgca gcacactaat ctggtcgaga tcgaaaaaat ataacccacg acaaccataa
    43561 attctggcat gacattttgt ttgaaaagca ataagtgagt aatgatgaaa atccatacca
    43621 cagaggcgct catgaaggct gaaatcagcc gtagaagtct gatgaaaacc tccgcacttg
    43681 gcagtcttgc gctggcaagc agtgctttca ctctgccatt ttcccaaatg gtccgggcgg
    43741 cagaggctcc ggtagaagag aaagcggtct ggagttcctg caccgttaac tgcgggagcc
    43801 gctgtctgtt acgtttgcat gtgaaagatg acaccgtgta ctgggtggag tctgatacga
    43861 caggtgacga cgtctacggt aatcatcagg ttcgagcgtg tttacgcggg cgctctatcc
    43921 gccgacggat gaatcatcct gataggttga aatatcccat gaagcgcgtc ggcaagcgcg
    43981 gtgaaggtaa atttgaacgg ataagttggg acgaagccct ggataccatc agtgataatc
    44041 ttcggcggat cctgaaagat tacggcaatg aggctgtaca tgtcctgtac ggaacaggcg
    44101 tagatggcgg aaacatcacc aactcaaacg tcccgtaccg tctgatgaac tcttgcggtg
    44161 gttttctcag tcgctatggc agctacagta ccgcacagat cagtgccgca atgagttata
    44221 tgttcggtgc caatgatggc aacagcccgg atgatatcgc caatacgaaa ctggtcgtta
    44281 tgttcggaaa taacccggca gaaacgcgga tgagcggcgg tggtgtcact tactacgtcg
    44341 agcaagcccg cgaacgttca aacgcacgca tgatcgtcat cgatccacgt tataacgaca
    44401 ctgctgccgg gcgtgaagat gaatggctgc ccattcgccc tggcaccgat ggcgcactgg
    44461 cctgtgcgat tgcctgggta ctgattactg aaaacatggt cgatcagcca tttctcgaca
    44521 aatattgtgt tggttacgat gaaaaaacgc tgcccgccaa cgcaccacgt aacgcgcatt
    44581 ataaagccta tattctgggc gaagggcctg acggcatagc taaaacgccg gaatgggcag
    44641 caaaaatcac cagcatcccg gcagaaaaaa ttatccagtt ggcacgagag atcggttcag
    44701 caaaacctgc ttatatttgt cagggttggg ggccacaacg acattccaac ggcgagcaaa
    44761 catcccgcgc tattgccatg ctttccgttc tcaccggcaa cgtcggcata aacggcggca
    44821 actcaggcgt acgcgaaggt agctgggatc tgggggtaga atggttcccg atgctcgaga
    44881 atcctgttaa aacgcagatt tccgtcttta catggacaga tgccatcgac catggtacgg
    44941 aaatgaccgc gacccgtgat ggtgttcgtg gaaaagaaaa actggatgtc cccatcaagt
    45001 ttttatggtg ctacgccagt aacacattga tcaatcaaca tggcgacatc aatcacaccc
    45061 atgaggtgct tcaggatgac agcaagtgcg agatgattgt tggcattgac cacttcatga
    45121 cggcctcggc taagtattgc gatatcctgt tgcccgacct gatgccaaca gagcaagaag
    45181 accttatctc tcatgaatct gcagggaata tgggctatgt gatcctcgcc caacccgcaa
    45241 cctcagcaaa atttgaacgc aaacccatct actggatgct gagtgaagtc gccaaacgct
    45301 taggaccaga cgtttatcaa acctttactg aaggtcgcag tcagcatgaa tggatcaaat
    45361 atctccatgc gaaaacgaag gaacgtaacc ctgagatgcc cgactacgag gagatgaaaa
    45421 cgaccgggat ctttaagaaa aaatgcccgg aagaacacta cgtcgctttc cgcgcattcc
    45481 gtgaagatcc acaggcaaac ccgttgaaaa caccttcggg gaaaatcgaa atttattctg
    45541 aacgactggc gaagattgca gatacctggg aattgaaaaa agatgaaatt attcatcccc
    45601 ttcctgcgta taccccaggt tttgatggct gggacgatcc cctgcggaaa acctatccac
    45661 tgcagttaac gggcttccat tacaaagcgc gtacccactc cagctacggc aatattgatg
    45721 tgttacagca ggcctgccca caagaggtgt ggatcaaccc cattgatgct caggcacgcg
    45781 gtatccgtca tggcgatacc gtgcgggtat ttaacaataa tggagaaatg ctgattgccg
    45841 caaaagtgac tccgcgtatt ctgcctggcg ttaccgccat cgggcaaggt gcgtggctta
    45901 aagcggatat gtttggtgac cgggtcgatc acggcggcag tatcaatatt ctgacctctc
    45961 accgcccttc accgctggca aagggaaacc cgtcgcacag caatcttgtc cagatcgaaa
    46021 aggtttaagg agtagcccat gaccacacaa tatggatttt ttatcgattc cagccgctgt
    46081 actggctgta aaacctgcga actggcgtgc aaagatttta aagatcttgg cccggaagtc
    46141 agtttccgcc gtatttatga atacgctggc ggcgactggc aggaagataa tggcgtctgg
    46201 caccagaacg tgttcgctta ttatctctcc atctcctgta accattgcga tgaccccgca
    46261 tgtacaaaag tctgccccag cggcgcaatg cataagcgtg aagatggctt tgtggtggtt
    46321 gatgaagatg tttgtatcgg ctgccgctac tgccacatgg cttgtccgta cggcgcgcca
    46381 cagtacaatg ctgaaaaagg gcacatgacg aagtgcgatg gttgttattc gcgcgtcgcc
    46441 gaggggaaac aacccatatg tgtcgaatcc tgcccgctgc gtgcacttga gttcggtcca
    46501 attgaagagc tgcgccagaa gcacggcact cttgctgccg tcgcgccact gccgcgcgcg
    46561 catttcacaa aacccaatat cgttatcaaa cctaacgcca acagccgccc gaccggcgat
    46621 accactggtt atctggctaa tccggaggaa gtgtaaaatg ggaaatggat ggcatgagtg
    46681 gccactggtt atctttaccg tattgggtca atgcgtagta ggcgcgctga ttgttagcgg
    46741 aattggctgg ttcgccgcca aaaatgacgc agaccgtcag cgtattgtcc gtggcatgtt
    46801 tttcctctgg ctgttgatgg gcgtcgggtt tattgcctct gtcatgcacc tgggatcgcc
    46861 actgcgggcg tttaactcgc tgaatcgaat cggagcttct ggtctgagta acgagattgc
    46921 tgccggctcc atattttttg ccgttggcgg attatggtgg ctggtggcag tcatcggtaa
    46981 aatgccgcaa gcgttgggga aactctggtt acttttcagc atggcgctcg gcgtcatttt
    47041 cgtctggatg atgacctgcg tgtatcaaat cgacaccgtg ccaacctggc ataacggtta
    47101 cactacgctg gctttcttcc tgacggtatt gttgagcggt ccaatacttg ctgccgcaat
    47161 tctgcgggca gctcgcgtta catttaatac cacaccattt gccatcatta gcgttctggc
    47221 gctgatcgct tgtgccgggg tgattgtgct gcaaggtttg tctctggcgt ctattcactc
    47281 atccgtgcaa caagccagcg cactggtacc agattacgcc tccttacagg tctggcgtgt
    47341 ggtattgtta tgcgcaggtc ttggatgctg gctttgcccg ctcattcgtc gccgtgaacc
    47401 tcacgttgcc ggacttattc tggggctaat cctcatactt ggcggtgaga tgatcgggcg
    47461 tgtgctcttt tatggcctgc acatgacagt aggaatggct attgcaggtt aacaaattgg
    47521 tgcggggcaa cccgcacatt tcaggatgtt aggaatgacc catttttcac agcaagataa
    47581 tttttctgtc gcggcgcgcg tgttgggcgc gctgttttat tacgctccag agagtgcgga
    47641 agccgcacct ctggttgcgg tactcaccag tgacggctgg gaaactcagt ggcctctacc
    47701 agaagcgtca ttagcgccgc tggtgacggc ttttcagaca cagtgtgaag agactcacgc
    47761 ccaggcctgg cagcgtttgt ttgtcggccc gtgggcactg ccgtctccgc cgtgggggtc
    47821 ggtctggctg gatcgcgaat ctgtgctgtt tggcgattca acattggcac ttcgtcagtg
    47881 gatgcgcgag aaaggcattc agtttgaaat gaagcaaaac gaaccggaag atcattttgg
    47941 ttcactgctg ttgatggctg cgtggttggc ggagaatggt cgccagacag aatgcgaaga
    48001 attgctggca tggcaccttt ttccgtggtc aacacgtttt cttgatgttt ttatcgaaaa
    48061 agcagaacac cctttctacc gtgcactggg tgaactggct cgcttaacgc tggcgcaatg
    48121 gcaatcacaa ctgttaattc ctgtcgcggt taaaccgctg tttcgatagg atcaccgtaa
    48181 tattgccgga tggtgacgat tacgcgcaat ccggcaataa taggttacag tgtcacgttt
    48241 ttttatctct taaagcacgc actgcttttg cggctggcct cttttgccgc aaaatagtcg
    48301 cccgtgtttc attgcccatt tctgctcatg catcatctac acatctatcc ggatctgcgc
    48361 actatgttcc gccgtctgct tatcgcaaca gtcgtcggta ttctcgcggc ctttgccgtt
    48421 gccgggtttc gtcatgcgat gctgctactg gagtggttgt tcctcaataa tgactccggc
    48481 agtctggtca atgcagcgac aaacctttcc ccctggcgac ggttgctaac tccggcgctc
    48541 ggcggactgg cggcgggttt gttgctgatg ggctggcaga aatttaccca acaacgccct
    48601 catgcgccga ccgattatat ggaagcgttg caaaccgatg gacagttcga ttacgcagca
    48661 agcctggtta aatcgcttgc ctctctgctg gtagtaacca gcggcagtgc aattggtcgc
    48721 gaaggtgcga tgattctttt agctgccctt gccgcctcct gttttgccca acgttttacg
    48781 ccacgccagg agtggaaatt atggatcgcc tgtggggccg cggcgggaat ggctgcggcc
    48841 tatcgtgccc cgcttgctgg cagtttattt atagccgaag tgctgtttgg cactatgatg
    48901 ttggcctctc tcggcccggt gattatttcc gccgtcgtgg cattgctggt tagcaatctg
    48961 attaatcata gcgacgcgtt actctacaac gtacaactct cagtgacggt tcaggctcgt
    49021 gactatgcgc tgattatcag tacaggtgtg ctggcaggtc tgtgcggacc actgttgtta
    49081 acgttaatga acgcctgtca tcgtggattt gtgagtctca aacttgcgcc gccctggcaa
    49141 ctggcactag gcgggttgat cgtgggtctg ctttccctgt tcacacctgc agtgtggggc
    49201 aacggctata gcaccgtaca atccttttta accgccccac cactgttaat gatcattgcc
    49261 gggatcttcc tttgtaaact gtgtgccgtg ctggcgagta gcggttccgg cgcacccggt
    49321 ggggtcttta caccgacgct atttatcggt cttgccattg gcatgttgta tggtcgtagc
    49381 ctgggattat ggttccctga tggcgaagaa attacacttt tactcggatt gaccgggatg
    49441 gcgacactgt tggcggcgac cacgcacgcg ccgattatgt cgacgttgat gatatgtgaa
    49501 atgaccgggg agtatcagct actccccggt ttattgattg cctgcgtaat tgcgtcggta
    49561 atttcgcgga cgttacaccg tgactctatc taccgccagc acactgcgca gcatagctaa
    49621 gcggatgtat tgccccagtt cacgctgttc ggcgcgcggc agataaggca gttcaccaat
    49681 gagcggtgcc ggaagttttt tacccagcac atcaatgatt tccgcataat gcgccagtcc
    49741 tgggttgatt cggttagcca cccagccaat gagcggcagc ccgtcgttgg cgatcgcctg
    49801 agctgttagc agtgcatggt taatgcaacc ttcctgaata ccgacaacca tcaacaccgg
    49861 cagttgttcc tgcactaccc attcagagag tggacgcaaa tcattcatca gactgcgcca
    49921 gccgccagtc ccttctacca cgacatgatc gactttttcg gtcaggtttg ccaggccgtt
    49981 tgaaatgagg gtgtaattga ttgggcaact gtgcgccacg ctactttctt cttcgcttaa
    50041 cgcgatagga ttaactgctt cataaggcag ttcgatggtt gaaacactct gcaacaccag
    50101 ggcatcttta ttacgcagcc cttcgggtgt ctctttgctc cccttcgcta cgggtttata
    50161 tcccgcaacc gtttttccct gggaggctaa cgcttgtagc aatgcgcggg aaaccaccgt
    50221 tttccctaca gaagtgtctg taccggtaat aaagaaacgc ttcagcatca ctaactccac
    50281 cgttatgctt cacaaatata aaccaggaaa ataattaacc ttgaaagtct aagttatgct
    50341 ttcctggccc aaattgagat agcgcaaatt ttggtagaac agttaaaaaa tgttaaccct
    50401 gcaacagacg aatcaacaaa gaaccgttat acatcgcgtc ttttaccagt gcagcgcctg
    50461 ccatcgtgcc ctggttagaa aactgagtac tctcaacgct gatgtgctga ctatacgcag
    50521 gaagggcctg ctgacggatg ctgtctgaga tgaccgggaa gaggatatct gccgctttac
    50581 ttaacggtga gccaatcagt attttttgtg ggttaaataa attcaccatg atggcaagaa
    50641 tgcgcccgac atgcgcgccc accccggtaa tgatgtcttt tgccagtaga tcgccgcgca
    50701 atgccgcctg acacaatgag tccacggtta acggttgtcc atgtaacatc gagctcatgg
    50761 attgattaag acgcagctgt gccagctcaa gaatactgtc cacgctggcg atggtttcga
    50821 ggcagccgtg attcccgcaa taacagcgtt tcccatacgg gtcgacctgt gtgtggccta
    50881 tttccacgag actactgctg cctgcgtgta gcagatgacc atcggtaatg acgcccgccc
    50941 ccacgttgtg atcgataacc acctgaatca catcgcgcgc cccgcgtgag gcaccaaaca
    51001 aggcctctgc catcgtccat gcgctgatat catgctgaat ataaaccgga acgccggtat
    51061 gctgctccag cgcctcgccg agcggcatct cttttacatc ctcgtagaac ggcatgcgat
    51121 gtacaatacc attttccgta tcaataattc ccggcaaggt tatggcaatc gaagttagac
    51181 gctcaagttt tttctggtgg cggataaaaa actgatcgat atgggaaata atacgatcca
    51241 gcaatggcaa gtcatctttt aacgccagtt cctgcgactc ttccaccacc agtttgctgc
    51301 tcagatcgcg cagagcaagg aaaatctccc cgcgactaat gcgcagagaa agatagtgcc
    51361 aggcttcagt ttcaaccacc agccccaccg ccggacggcc acggttcccc gcttctttga
    51421 tttccagctc ttgcaccagg tgtgcttcga gcatctcacg gacaatttta gtgatactgg
    51481 caggagccag ttgcgccaga cgggaaagat cgatacgcga gactggacca agctgatcaa
    51541 tcaggcgata aaccgcgccc gcgttggtct gctttatttg atcaatgtgc ccaggctggt
    51601 tttcagcaac caccgcatac tccctatatt ttcgcgctcc gaaataatct gtaggctatg
    51661 gtgaagcact tcaatacgtg tcgtcaaatt tttacttagg catgtgatta acagcacatt
    51721 tttcgggctt tttcgctgaa atttccctca cctgagagca ttcagcagat gaatacgaaa
    51781 gttacgcgca gccggacttt gttcatgatg tttcggccag accaaccaca tttcagaaac
    51841 cgcatcctct tcagcaatcg gcacccagcg catttcgttg agctgaacac gtttaaatga
    51901 cgcaggcaaa attgaaacac ccagaccggc ggaaaccaga ccgatgatgg tcattgcctc
    51961 gcccacctcc tgagtgatga cgggcgtcaa atggtaacgt cgcatcagcc cgagaatatc
    52021 gtcatacagc cctgtcccga cgtgcggatc aaaaaagaca aagggttctt tcgccagttc
    52081 agccagcgtt acattcgggt tatttgccag gggatgatcg tgcgggatca tcgccataag
    52141 cggttcatgg acgatgactg cgtgttcaag cgactccggt aacgctgtgt tacgcagcaa
    52201 tcccatatcc agcgttcctt caatgagcgg agcgatttgc tcgcgagtgt tcatttcgcg
    52261 ggtttgtaaa tggacatcag gataatcacg gcgaaacagc gataacgtat cggacaccgc
    52321 ccgaataaaa ggagccgacg aagtaaaacc aatgcgcaac tcccccgctt caccctgatg
    52381 cagcctttca gcgcgagcgg cagcgtcatc caccatagac aggatttgcc gactatctgc
    52441 aagaaactgt tttcctgctg ccgtcagcaa tacactgcga ttggttcgtg ccagcagtcg
    52501 ggcaccaatt tgttgctcca gcgcctgaat ctgctgactt agcggcggtt gcgaaatatt
    52561 caggcgggca gcggcgcgcc cgaaatgcag ctcttccgca acagcaacaa agtaacgcag
    52621 atgacgaagt tcaatattca tatttaaaac atcttatttg agattattaa tatattagac
    52681 agaacaattc gattttccta ccctatgtat aagcctgatc tacaggcata tttagcaagg
    52741 atttcaagtg agccgtacta caactgttga tggcgctccg gcaagcgaca ctgacaagca
    52801 aagcatttct cagccaaatc aatttattaa acgcggtacg ccgcaattta tgcgcgtcac
    52861 cctggcgctg ttctctgccg gactggcaac atttgcactt ctctattgtg tgcagcctat
    52921 ccttccggtg ctttcgcagg agtttggctt aacccccgcg aacagtagta tttcactgtc
    52981 catttccacg gcgatgttgg ctattggttt gctgtttact ggcccgctat ccgatgccat
    53041 tggtcgcaaa ccagtgatgg tcacggcgct actgttggcc tccatttgta cgttactttc
    53101 gacaatgatg accagctggc acggcatttt gattatgcgc gccttgattg ggctttcgtt
    53161 aagtggcgtg gcagctgttg gcatgactta tcttagcgag gaaatccatc ccagtttcgt
    53221 ggccttttca atggggttgt atatcagcgg caactcaatt ggcggcatga gcggacgctt
    53281 aattagcggt gtcttcacgg actttttcaa ctggcgaatt gctctggcgg caatcggttg
    53341 tttcgcgctg gcctcggcgt tgatgttctg gaaaatcctc cctgaatcac gccattttcg
    53401 cccgacttcg ctgcgcccta agacgttgtt tatcaacttt cgtctgcact ggcgtgaccg
    53461 gggattaccg ttattgttcg cagaaggctt tttgctgatg gggtcgttcg tcacgctgtt
    53521 taattacatc ggctatcggt tgatgctctc cccctggcat gtcagtcagg ccgtggttgg
    53581 cttattatcg ctggcttatt tgaccggtac atggagctca cccaaagccg gaaccatgac
    53641 cacccgctat gggcgtggtc cagtgatgtt gttttcgacg ggggttatgc tgtttggttt
    53701 actgatgacc ttattcagct cgctgtggct gatctttgcc ggaatgttac tcttctcagc
    53761 aggattcttc gcagcccact cagtagccag cagctggatc ggcccccgcg caaaacgcgc
    53821 taaaggccag gcctcctcgc tgtatctgtt cagttactat ctggggtcga gtattgccgg
    53881 gacgctgggt ggtgttttct ggcataacta tggctggaac ggcgtcggcg catttattgc
    53941 tctgatgctg gtcattgctc tgctggtcgg gacgcgtttg catcgtcgtc tgcacgcctg
    54001 aaaaataagt ccggactgcg gtaaataccc gtccggactt attgccagct caaaccaacg
    54061 ttaatagcca tcctaaaata gacgaagcgc cagccaattc ccgcagcgcg tctagcgtca
    54121 tcaggattat aagtacccaa ataaacggat gcattttgct gtgtgtcatt tattactgat
    54181 gcgcagttat tctactgctt tgtaagtagt aaaatagtta acccgatcaa gactactatt
    54241 attggtagct aaatttccct taagtcacaa tacgttatta tcaacgctgt aatttattca
    54301 gcgtttgtac atatcgttac acgctgaaac caaccactca cggaagtctg ccattcccag
    54361 ggatatagtt atttcaacgg ccccgcagtg gggttaaatg aaaaaacaaa ttgagggtat
    54421 gacaatgaaa aaagtattag ctctggttgt tgccgctgct atgggtctgt cttctgccgc
    54481 ctttgctgca gagactacga ccacacctgc tccgactgcg acgaccacca aagcagcgcc
    54541 ggcgaaaact acacatcata aaaaacagca taaagcagca cctgcccaga aagcgcaggc
    54601 ggctaaaaag catcataaaa atacgaaagc tgaacagaaa gcccctgaac aaaaagcgca
    54661 ggcagcgaag aaacacgcca agaaacacag ccatcagcaa ccggcaaaac ctgctgcaca
    54721 acccgcagcg taagttttca acagtaatgc tggcgcgccc cctcgcgcct gaaaattacg
    54781 gtgctaagcg ggtaacgttt agcaccgcct ttagcggagg gtagtatggt gggccgttat
    54841 cgctttgagt tcattctgat catccttatt ttatgcgcac tgattgccgc ccgtttttat
    54901 ctttcctgat tgtagttatc tgattttact cccactttca tcccgtcccg tctatagtat
    54961 ttatgagggt ttgcttttaa taatcataat tacccaccag agtgtgatat gcgtacaacc
    55021 attgctgtag tgttgggtgc aattagtttg acgtctgctt ttgtgtttgc agataaacca
    55081 gacgttgcca gatcggcaaa cgatgaggtc agcaccctgt tttttggtca tgatgatcgt
    55141 gtgccagtga atgacacgac ccaatcaccg tgggatgcgg ttgggcaact ggaaacggcc
    55201 agcggcaatt tatgtacggc gacgctgatt gcacccaatc tggcattaac ggcaggacac
    55261 tgtttattga cacctccaaa gggtaaagcc gataaagcag tggcgctgcg ttttgtgtca
    55321 aataaaggtc tttggcgcta tgagatccac gacatagaag gccgcgttga tccgacactg
    55381 ggaaagcggt taaaagcaga tggggatggt tggattgtac ctcccgcagc cgcgccgtgg
    55441 gacttcggat tgattgtgct acgtaatccc ccttctggca ttacgccgtt gccgttattt
    55501 gagggagata aagccgcgct taccgccgca ttaaaagcgg caggtcgtaa agtgactcag
    55561 gcaggctacc ctgaagatca tctcgatacg ttgtacagtc atcaaaactg tgaagtgact
    55621 ggctgggcgc aaacgtcggt gatgtcacat cagtgcgata ccttgccggg tgacagcggt
    55681 tcgcctctga tgttgcatac cgatgacggc tggcaattaa ttggggtgca aagttcggct
    55741 cctgccgcga aagatcgctg gcgcgccgat aaccgggcca tttctgttac cggttttcgc
    55801 gacaagctgg atcaactgtc gcaaaaataa tgttcaaacg ctgcccgaca gcgcgggcag
    55861 cgtcttcatc aggcaagttt caccatgatc attccagcca acagcaagac caggccaatc
    55921 cagcctttac gatttaaccg ttgaccaaac aagatccaac ctgcggctaa cgtggcggca
    55981 ataccaaacc cgccccacaa tgcataagcg acagacaagt cgatcccttt aacggcttga
    56041 gaaagcgcac taaaggcagc cagcaccgcc gccagggaga gcaagccaaa tattttgcga
    56101 cgaaagccgt cagaaaattt caaaaagacg ttagcaacga tttccagcac gattgccaat
    56161 gccagccagg cggcgtgaac ccattcaaac tgcgccatgg ttcacctcca gttcaggttt
    56221 acgcgcttta cgggtacctg attttatcaa cacaatcccg gcgaccaggg tggttaaccc
    56281 ggcaattttc atcagcgata aactttcgtc gaataacaaa acgctaaaca aggtaataaa
    56341 taaaataccg ataccttccc acagcgcata agctacgcct aaggcgattt ttttaacggc
    56401 gaaagagaga aatatatacg acagagaaat catcaccagc attaaaataa agccgccatt
    56461 tccctcactg acgctcgccc atttcattga cagcgtaccg gtaatttctg tagcaatagc
    56521 cagacctaat aaaatccaat aaatatacat tgtccttctc ctgcaagaga attattttaa
    56581 ttttcgctta attcagcgaa accaaagtaa agtggtcaag ccccgcgcat agcgcaagct
    56641 ttggcagaag aaaggactaa agcgcgttgc gccagtgctg ctcacctacg agcaagatag
    56701 tggatgaggt acgaagatgg gtaaatgtag aaaataacgt cctgaacaaa ttgtccataa
    56761 tattacaatt atccgcagtg ttgcttctcg tcatcgcgga tgataattgt cctcggtagt
    56821 tgaacacgcc tgatttgtat catagcttaa gaattaactc aaaatatttt cacttcttta
    56881 cctgagcggt ttgattttcg ttatgatgac ggagcgaaaa agacattatt attagcaaag
    56941 gaagaaaaaa cggggacaag catggcaaag ccgatcatca cgctcaatgg cctaaaaatc
    57001 gtcattatgt tgggaatgct ggtcattatt ctctgcggta tccgttttgc cgccgagatc
    57061 atcgtgccgt ttattctcgc attatttatt gctgttattc ttaacccgct ggtgcaacac
    57121 atggtccgct ggcgtgtgcc gcgtgtactg gcggtgtcga ttttgatgac catcatcgtg
    57181 atggcgatgg tgttgctatt agcttatctg ggttccgcgc tcaacgagtt gacgcggacg
    57241 ttaccgcaat atcgcaactc tattatgacg ccgctgcaag cgcttgaacc gttgttgcaa
    57301 cgcgtaggga ttgacgtctc agttgaccag ctggcgcatt atattgatcc gaacgcggcg
    57361 atgacgttgc tcaccaactt attgacgcag ttatctaatg ccatgtcatc aatattttta
    57421 ttgctgctga cggtgctgtt tatgctgctc gaagtgccac aattgcccgg aaaatttcag
    57481 caaatgatgg cgcgtccggt tgaagggatg gcggcgattc aacgtgcgat tgacagtgtt
    57541 tctcattatc tggtgctgaa aacagccatc agcatcatca ccggcctggt cgcctgggcg
    57601 atgctcgccg cactcgatgt tcgcttcgct tttgtctggg gattgctggc ctttgcgctt
    57661 aattacatcc cgaatattgg ttcagtcctc gcggcaatcc cccctatcgc tcaggtactg
    57721 gtgtttaatg gcttctacga agcgttgctg gtgctggcgg gatatctgct gattaatctg
    57781 gtcttcggca atattctgga gccgcgtatc atggggcgtg ggctggggct ttccacattg
    57841 gtggtatttt tgtcgttgat tttttgggga tggttgttag gaccggtggg tatgctgctt
    57901 tccgtgccgt tgacaattat tgtcaaaatt gcgcttgaac aaacagcggg aggtcaaagc
    57961 atcgccgttc tgttaagcga tctcaataaa gagtgacggc ctcagcagag gccgtcaggg
    58021 ttacagagct ttcaggattg catccacgct ggctttggcg tcaccaaaca gcatgtgggt
    58081 gttttccttg aagaacagcg ggttttgcac accagcatag ccagtgttca tcgaacgttt
    58141 aaagacaatc acgttctgcg ctttccacac ttccagcaca ggcataccag caatcggact
    58201 cttcggatca tcctgcgccg ccgggttaac cgtatcgtta gcaccaatca ccagtacggt
    58261 atcggtatca gcaaagtcat cattgatctc gtccatttcc agcacgatgt catacggtac
    58321 ttttgcttca gccagcaata cgttcatatg tccaggcaaa cgccccgcga ccgggtggat
    58381 accgaaacgc acattaatac cacgagcgcg caatttctca gtaatttcag cgacaggata
    58441 ttgcgcctgc gcgactgcca tgccgtaccc cggagtaatg atcactgaat gggagttttt
    58501 cagcagttcc gctgtctctt ctgcggtgat ttcgcggtgc tcacccactt cctgatcatc
    58561 gccagtagaa gagccgtcgg tgccgaaacc acccgcaata acgctgataa aggaacggtt
    58621 catcgcctta cacataatgt aagaaaggat agcccccgaa gaaccgacca gcgcaccggt
    58681 cacaatcagc aggtcgttgc tgagcataaa gcccgcagcc gcagccgccc agccggagta
    58741 cgagttcagc atcgacacca ccactggcat atctgcacca ccgatggagg cgactaaatg
    58801 ccagccgaat accagcgcaa ttgcggtcat tatcagcaat gccagcactt gcaggccgac
    58861 gctgtccgtg cgaacaaata caatcagcag caggaaggaa acgaccagag ccgccaggtt
    58921 cattttgtga cggtttggca gcatcaatgg tttagacgaa atcttgccac acagtttgcc
    58981 gaacgccacc accgaacccg tgaacgttac cgccccgatg aagataccga ggaacacttc
    59041 cgtcaggtga atattgacca gaatcggtgc cattcccgcg tcatgatgca gatagctgtt
    59101 aaagccaacc agcactgccg ccagacccac gaagctatgc aggatcgcca ccagttctgg
    59161 catttcggtc atttcaactt tcttcgccag acggatacca attgccccac caatgaccat
    59221 cgccagcaag atccagccaa cattacccgt atccggtcca aaaatggttg cgattaacgc
    59281 aatcgccatc ccggcgatac cgaagttgtt accctggcga gacgtttcat gtttcgaaag
    59341 accggccaga ctgaagataa acaggatcgc ggcaacaatg tatgcagctg taactaatcc
    59401 tccagacata tgttacccct taatttttgc ggaacatttt cagcatgcgc tgagtcacgg
    59461 tgaagccacc gaaaatatta atgctggcta taagcaccgc gataaaacta aggaagctaa
    59521 cccagccgcc ctggccaatc tgcaacagtg ctccgacaac aataatccct gaaatcgcgt
    59581 tggtgaccga catcaacggt gtatgcagcg cgtgcgatac attccacacc acgtaataac
    59641 cgacaacgca ggccagcgcg aaaacggtga agtgcccaag gaattctttc ggcgcaacgc
    59701 ttgccatcca gccaaaaaga atgattgcca gcgccatcaa cgcgtattta cgccacggtg
    59761 agcaggtaca tttttcctca gttttcactt ccggtgccgc tttttgtgcc gcctgcggct
    59821 gagctgatac ctgaatcggc ggtgccggcc aggtaatttc gcccgcacgg atcacggtca
    59881 cgccgcgaat caccacatca tcaaaatcaa cagtgatatt gccgtctttc tctttgcaca
    59941 acagtttcag cagattaacg aggtttgtgc cgtaaagctg tgaggattgc gtcggcagac
    60001 ggcccggaag atcggtataa ccaatcactt tgacaccatt ttccgtagtg aagatttcac
    60061 ccggcacggt gtattcacag ttgccgccgt tttgggctgc caggtcgaca atcacactgc
    60121 ccgccttcat ggagtcaacc atttcacggg taattagctt cggcgctggt ttgcctggaa
    60181 taagcgcggt ggtgacaatg atatcgacct cttttgcctg ggcggcaaag agttccattt
    60241 ccgctttgat gaacgcgtcc gacatcactt tggcatagcc atcgccgctg ccagcttcct
    60301 ctttaaaatc cagctcgagg aattccgcgc ccatactttg aacttgttct ttcacttccg
    60361 ggcgggtgtc gaatgcacgc acaatcgcgc cgagactgtt tgctgcgcca atggcggcca
    60421 gacctgcaac acccgcacca atcaccatca cttttgccgg tggcactttc ccggccgcag
    60481 taatttgccc ggtaaagaag cgcccaaatt catgtgccgc ttcaacaatg gcgcgataac
    60541 cggcgatgtt cgccatcgag cttagtgcgt ccagcgattg tgcgcgtgag atacgcggca
    60601 cagagtccat cgccatcacg gtcacgttac gttccgcaag tttttgcatt aattccggat
    60661 tctgcgcagg ccagataaaa ctcaccagcg ttgtcccagg attcagtaac gcaatttcat
    60721 catctaacgg cgcattgacc ttcagaatga tctctgactg ccagacgcta ttcccttcta
    60781 caatttcagc gcccgcttgc acaaacgctt tatcgtcaaa acttgccagt tgacccgcgc
    60841 cgctctctac cgcgacggta aaacccagtt tcagcagctg ttccactgtt tttggcgttg
    60901 ctgcaacacg ggtttcattg gttaaccgtt ctcttggtat gccaattcgc atgatattcc
    60961 cttccatcgg ttttattgat gatggtttgc ctgtgtcagg agccacacaa gctgctcatg
    61021 tacgagctaa atgttactcc gttaaaataa attagtaaca aacgccctat aacgtactga
    61081 aaattatgcc tgtgatctag cgccaaaaat cagtatttcg gcgtgaactc gcaaaatatt
    61141 aacgattcag ccgtgatagt gggataaaca ccttagaacg ccggataaag actgataatt
    61201 gtcttcgacg gtcgggtaaa acgagacatc gccccggcac gaatcactac ttaacattaa
    61261 attaacttat acaattcagt tgcttcagta gtaatgatgc tgatacggct gttttttaag
    61321 catagacggt catttgagca ggattaaaat tggcttaagg aatgtgatat gaaaaatgac
    61381 gcagacagtt acaccgttta aatgcaataa tcagccacgt ttctcgttaa taacaatacc
    61441 agtacctggt ttgcgcaagg cgaaggatta tttttatgaa gcttaagaac accctcctgg
    61501 cgtcggcact gctttctgct acggcattct ccgttaacgc agcaacagaa ctgacaccgg
    61561 agcaagcggc agcggttaaa ccttttgacc gtgtagtggt taccggtcgt tttaatgcta
    61621 ttggcgaagc ggtgaaagcc gtttctcgtc gcgcagataa agaaggtgcc gcctcttttt
    61681 atgttgtcga cacttctgat tttggtaaca gcggtaactg gcgtgtggtc gctgacctct
    61741 ataaagccga tgctgaaaaa gcagaagaaa caagtaatcg cgtaattaac ggtgttgtcg
    61801 aactgccgaa agatcaggct gttctgattg aaccgtttga cacggtcacc gtccagggct
    61861 tctatcgtag ccagccagaa gtcaatgatg ccatcaccaa agcggcaaaa gcgaaaggtg
    61921 cctactcttt ctacatcgtt cgtcaaatcg atgccaacca gggcggcaac cagcgtatta
    61981 ctgcattcat ctataaaaaa gatgctaaga aacgtatcgt ccagagcccg gatgtgatcc
    62041 cggcagattc cgaagcagga cgtgcagctc tggctgccgg tggcgaagcc gcgaagaaag
    62101 ttgagatccc gggtgttgcg actaccgcat caccaagttc tgaagtcggt cgcttctttg
    62161 aaacccagtc atcaaaaggc gggcgttaca ccgtcacgct cccggatggc actaaagtcg
    62221 aagaactgaa caaagcgacc gcagcgatga tggtcccgtt cgacagcatc aaattctctg
    62281 gcaactacgg caacatgacc gaagtctcct atcaggttgc gaaacgtgcc gcgaagaaag
    62341 gtgctaagta ttaccacatc acccgccagt ggcaggaacg tggtaataac ctgaccgtca
    62401 gcgcagatct gtataaataa ctattcagtt actcctataa ggcggcttga tgccgccttt
    62461 cgcattttta gtcacaaatt tccccgcgac cattgcatgc agacacatct cttcgtaaaa
    62521 tcccgcgctt cagtggcaaa ctccattttt atgcgttttg ccctaatatt tattcttctt
    62581 atcacgtttt aatcactgga tatcgatgga aaagaaactg ggactgagcg cactcaccgc
    62641 gctggtatta agctcaatgc tgggcgcggg tgttttcagt ctgccgcaaa atatggcggc
    62701 agttgccagc ccggcagcac tgctcatcgg ctggggtatt actggcgctg gcattttatt
    62761 gctggccttt gccatgctga tcctcacgcg cattcgtccc gaacttgacg gcggtatctt
    62821 tacctatgcc cgcgacgggt ttggcgagct aatcggcttt tgttccgcat ggggatactg
    62881 gctgtgcgca gtcatcgcca acgtctccta tctggtgatc gttttttccg cgttaagctt
    62941 ttttacggac acgccggaat tgcgcctgtt tggcgatggt aatacctggc aatcgatagt
    63001 cggtgcatcg gctttattgt ggattgttca ctttttgatc ctgcgcgggg tgcaaaccgc
    63061 tgccagcatt aacctggtgg cgacactggc aaaattgttg ccgctgggtc tgtttgttgt
    63121 gctggcgatg atgatgttca aactggatac cttcaagctc gacttcaccg gacttgcact
    63181 tggcgtaccc gtttgggaac aggtgaaaaa caccatgctg atcaccctgt gggttttcat
    63241 tggtgtggaa ggtgccgtgg ttgtttctgc gcgtgcgcgt aataaacgtg atgttggcaa
    63301 agcgacactg ctggcggttc tctccgctct gggcgtttac ttgctggtaa cgctgctttc
    63361 gctgggtgtg gtggcacgtc ctgaactggc tgaaattcgt aacccgtcaa tggccggtct
    63421 gatggtggaa atgacgggac catggggcga aatcatcatc gctgccggtt tgatcgtttc
    63481 cgtttgcggc gcgtacttaa gctggaccat catggcagca gaagtaccgt tcctggccgc
    63541 cactcataaa gcattcccgc gcatttttgc gcgccagaac gcacaagctg cgccatccgc
    63601 ctcactgtgg ttgaccaaca tctgcgtgca aatttgtctg gtactcatct ggctgaccgg
    63661 ttccgattac aacacgttgc tgaccatcgc ctcagaaatg attctggtgc cctatttcct
    63721 ggtcggcgcg ttcttgctga aaatcgcaac ccgtccactg cataaagcgg taggtgtcgg
    63781 tgcctgcatt tatggcttat ggttattgta cgcctctggt ccaatgcact tgctgttatc
    63841 cgttgttctg tatgcaccgg ggctgctggt tttcctgtac gcgcgcaaaa cgcataccca
    63901 tgataatgta ctgaaccgtc aggaaatggt gctgattggc atgctgttaa ttgcctcagt
    63961 tccggcgacc tggatgctgg tgggataacg ttcatcccat cgttggatag caaaggagat
    64021 aacgatgggt aaaacccagc ccttgccaat attaattact ggcggaggtc gtcgcatcgg
    64081 cctcgccctc gcatggcatt tcattaatca aaagcaaccg gtgattgtca gctatcggac
    64141 acactatcca gccattgatg gactgattaa tgcaggtgcg cagtgtattc aggctgattt
    64201 ttcgaccaac gacggtgtga tggcgtttgc cgatgaagta ctaaaaagca cccatggtct
    64261 gcgtgctatt ttgcataacg ccagtgcgtg gatggcggaa aaaccgggtg cgccactggc
    64321 cgacgtactg gcttgcatga tgcagatcca cgttaatacc ccatacctgc tcaaccatgc
    64381 gctggaaaga ttactgcgtg ggcacggaca cgccgccagc gatatcattc actttaccga
    64441 ttatgtggtg gagcgcggta gcgacaaaca tattgcgtat gctgcaagca aagcggcact
    64501 ggataatatg acccgctcgt ttgcccgcaa gctggcaccg gaagtgaaag tgaattctat
    64561 tgcgccatcg ctgatcctgt ttaatgaaca tgatgatgcc gaatatcgac aacaggcgct
    64621 gaataaatca ctgatgaaaa ccgcgcctgg cgagaaagaa gtgatcgacc tggtcgatta
    64681 cttacttacc agttgctttg tcaccggacg cagtttccca cttgatggcg gtcgtcatct
    64741 gcgttaatgc agctttatcc agcatataat caacacccat gcgcttatcc cccaacacgc
    64801 aacagaacca acaaaagcgg cgggcaagcg catcattccg gtgaaatacc acagcgacac
    64861 caggtaaaca aaatagggaa taatcgacca catactaaaa atgatggttg cgcgtaaggc
    64921 ttcaatgccg cgttcgctgg caacaatata atgcgcgata agcgcaaagg tcgggaaaag
    64981 tggaatcagc ccggcgatat aataattttt cgtttttgct aaaacaccaa tcaacagcac
    65041 taccagcgca ccgagcgcgg ctttgattac cagccccatc tttttacctt aacacttcca
    65101 taacaagtca tcagtagaat acctgatgaa aacttgttta gaaacgattg atagtaagta
    65161 aaaacagcgc ggtgtattgt gacgttttta tatctaccgt gaatgttatg aacactatcg
    65221 tatttgtgga agatgatgcg gaagtcggtt cactgattgc cgcgtacctg gcaaaacatg
    65281 atatgcaggt taccgtagag ccgcgcggcg accaggccga agaaaccatt ttgcgagaaa
    65341 atccggattt ggtgttactc gacatcatgc taccaggcaa ggacggcatg accatttgtc
    65401 gtgatttacg cgcaaagtgg tctggaccga ttgttcttct aacctctctc gatagcgata
    65461 tgaaccacat cctggcactg gaaatgggtg cctgcgacta tattctcaaa acgacgcccc
    65521 ctgctgtttt gctagcgcgt ttacgtttgc atttgcgtca gaatgagcaa gccacactga
    65581 ccaaaggtct tcaggaaacg tctctgactc cctacaaagc cctgcatttc ggcacgttga
    65641 ccatcgatcc catcaaccgc gtagtcaccc tggctaacac tgaaatctcg ctctcgacag
    65701 ctgatttcga attattgtgg gaattagcta cccatgccgg gcaaatcatg gaccgcgatg
    65761 cattgctgaa aaatttacgc ggcgtcagtt atgacggact ggatcgtagc gtggacgtgg
    65821 ctatttcgcg gttaagaaaa aaactgctcg ataacgccgc agaaccttat cgcattaaaa
    65881 ctgtgcgtaa caaaggctat ctttttgcgc ctcatgcatg ggaataagcg atgaaaaaac
    65941 tgtttatcca gttttacctg ttattgtttg tctgcttcct tgtgatgtct ctgctggttg
    66001 ggctggtgta caaatttacc gccgaacgcg cgggcaaaca gtcgctggat gatttgatga
    66061 acagttcgct gtatctgatg cgcagcgaat tgcgtgagat ccccccacac gactggggta
    66121 aaactctgaa agagatggat ttaaatctct ctttcgatct gcgtgtcgag ccactgagta
    66181 aataccatct tgatgatatt tccatgcacc gactgcgtgg cggcgaaatt gtcgccctgg
    66241 acgatcagta cacgtttttg cagcgtatcc cgcgcagcca ctacgtgctg gcagttggtc
    66301 ctgttcctta tctttattat ctccatcaga tgcgattgct ggatatcgcc ctgatcgctt
    66361 ttattgctat ttccctcgcc tttccggtgt ttatctggat gcgtccgcac tggcaggata
    66421 tgttaaaact ggaagcagcg gcgcaacgat ttggcgatgg gcatctcaat gaacgtatcc
    66481 actttgatga ggattcgagc tttgaacgac ttggcgtcgc atttaaccag atggcggaca
    66541 atatcaacgc cttaattgcc agcaaaaaac agcttattga cggtatcgct cacgaactgc
    66601 gaacaccgtt agtgcgcctg cgttatcgac tggagatgag cgataacctg agcgccgccg
    66661 aatcccaggc gttgaatcgt gatatcagtc aacttgaagc tttaattgaa gagctgctga
    66721 cttatgcccg actcgatcgc ccacaaaacg agcttcatct tagcgaacca gacctgccgt
    66781 tgtggctgtc aacgcatctg gcagatattc aggcagtaac gcccgataaa acggtacgga
    66841 taaaaacgct cgtgcaaggc cattatgcgg cgttggatat gcgcttaatg gagcgcgtgc
    66901 tggataattt gctcaataac gccctgcggt actgccattc aacggttgaa accagcctgc
    66961 tactgtcggg gaatagagcg acattaattg ttgaggatga tggcccaggg attgccccag
    67021 aaaaccgcga acatatcttt gaaccttttg ttcgcctcga tcccagccgg gatcgctcaa
    67081 ccggcggctg cgggctgggg ctggcaattg tccactctat agcactggca atgggcggta
    67141 cggttaattg tgacaccagc gaactgggtg gtgcccgctt ctcgtttagc tggccgttat
    67201 ggcataacat cccgcaattt acctctgcct gacactacgc gcacgatggt caagtcacca
    67261 cgactgtgct ataaaataag tatgttgtaa ctaaagtggt taatattatg gcgcgttacg
    67321 atctcgtaga ccgactcaac actacctttc gccagatgga acaagagctg gctatatttg
    67381 ccgctcatct tgagcaacac aagctattgg ttgcccgcgt gttctctttg ccggaggtaa
    67441 aaaaagagga tgagcataat ccgcttaatc gtattgaggt aaaacaacat ctcggcaacg
    67501 acgcgcagtc gctggcgttg cgtcatttcc gccatttatt tattcaacaa cagtccgaaa
    67561 atcgcagcag caaggccgct gtccgtctgc ctggcgtgtt gtgttaccag gtcgataacc
    67621 tttcgcaagc agcgttggtc agtcatattc agcacatcaa taaactcaag accacgttcg
    67681 agcatatcgt cacggttgaa tcagaactcc ccaccgcggc acgttttgaa tgggtgcatc
    67741 gtcatttgcc ggggctgatc acccttaatg cttaccgcac gctcaccgtt ctgcacgacc
    67801 ccgccacttt acgctttggt tgggctaata aacatatcat taagaattta catcgtgatg
    67861 aagtcctggc acagctggaa aaaagcctga aatcaccacg cagtgtcgca ccgtggacgc
    67921 gcgaggagtg gcaaagaaaa ctggagcgag agtatcagga tatcgctgcc ctgccacaga
    67981 acgcgaagtt aaaaatcaaa cgtccggtga aggtgcagcc gattgcccgc gtctggtaca
    68041 aaggagatca aaaacaagtc caacacgcct gccctacacc actgattgca ctgattaatc
    68101 gggataatgg cgcgggcgtg ccggacgttg gtgagttgtt aaattacgat gccgacaatg
    68161 tgcagcaccg ttataaacct caggcgcagc cgcttcgttt gatcattcca cggctgcacc
    68221 tgtatgttgc agattaacgc ccggctttca tactgccgac catctgttct ggccgtaccc
    68281 agctgtcaaa ctcggcttcg ctaagatacc ccagcgcaag ggccgcagct tttaaggtca
    68341 gcccttcttt atgcgctttt ttggcgatct cggcggcttt gtcataacca atgtgggtgt
    68401 taagcgcagt caccagcatc agcgattcat tgagtaattg attgattcgc tcacgattcg
    68461 gttcaatacc cactgcgcag tgtttgttaa aactttccat gccatctgcc agcaagcgca
    68521 ccgattgcag gaaattgtgg atcaccattg gacggaagac gttcagttca aagttaccgg
    68581 aagcgccccc catgttgatc gccacgtcgt tccccatcac ctgacagcag agcatggtta
    68641 atgcctcaca ctgtgttggg ttcactttcc ccggcatgat tgagctgccc ggctcatttt
    68701 ccgggattga gatttcacca attccgcagc gcgggccaga ggccagccag cggacatcat
    68761 tggcgatttt catcagtgac gcagccaacc ctttcaacgc gccgtgcgcc tgaaccaggg
    68821 catcacaggt cgccagcgct tcaaatttgt tcggcgcggt aacaaacggt gcacaggtaa
    68881 tgactgccag ttcatctgct acgcgacgcg catactccgg atgggtattt agtccagtac
    68941 ccaccgctgt accgccaaga gccagttccg ctacgtgagg caggctgtat tcgatatgtt
    69001 tgagattatg ctcgagcatc gctacccagc cggaaatctc ctgccccagc gttaacggcg
    69061 tggcatcctg caagtgagta cgaccaattt tgacgatatc ggcaaaagca cgggatttct
    69121 cattcagtgt ctgtgtcagg gttttaagct gaggaatgag ttgcttgcgc agcgccagca
    69181 gcgccgcaac gtgcatcgcc gtcggaaaga catcgttgga actttggctt ttgttcacgt
    69241 cgtcgttagg gtgaacttta cgttccatcc cgcgcacacc gccgagtaat tcactggccc
    69301 ggttagccag cacttcgttc atgttcatgt tactttgcgt gccggagccg gtctgccaga
    69361 tagccagcgg gaattcgtcg tcatgctgtc ctgccagtac ttcatccgcc gcctgacgaa
    69421 tggcgctcgc tttctcttca gacaacaagc ctaaatcttc attaactttt gccgctgcac
    69481 gcttggttag cgccagcgca tgaatcagtg aggtgggcat tttctccgtc gaaatgcgga
    69541 aatgctccag cgagcgttga gtttgtgcgc cccacagctt atctgccggg acatcaatcg
    69601 cccccatcga atctttttcg ctgcgtactg tattcatgac ctgctcctca cctgattaat
    69661 tttttctttc tgttttgctt tcgttaagca acttttagct cacttattat ttaccatttg
    69721 ataacaaatg tttggtcttt cgtgccatgt aaaaaaaccg ccccgaaggg cggctctgtt
    69781 tatttcacac agcgggtgca ttgtgtgagt tgtatctgct ggaagaagtc atttccttta
    69841 tcatccacaa ggataaacgc cgggaaatct tccacttcaa ttttccagat ggcttccatt
    69901 cccagttccg gatattcaac acattccagg ctcttaatac ttccctgcgc caatacagcg
    69961 gccggaccac cgatactgcc aaggtagaag ccgccgtgtt ttttacaggc atccgtcacc
    70021 tgctggctgc ggttgccttt cgccagcatg atcatacttc cgccctgcgc ttgcagttga
    70081 tcgacataag aatccatccg tccggcggtc gttgggccaa gagaaccgga ggcataacct
    70141 tccggcgttt tggccggacc cgcgtagtaa atcggatgat ctttgatgta ctgcggcagc
    70201 ccttcaccgt tatccatccg ctctttcagt ttggcgtgag caatatcacg accgacgata
    70261 atcgtgccgt taagcgataa gcgtgtagaa acgggatact gcgacaactg tgcgaggatc
    70321 tctttcatcg gacggttaag gtcaacgcgc accgcttcgc cttctcccgc tttgcgcagc
    70381 tcttccggga tatatttgcc tggattatgt tccagttttt cgatccagat cccctgacgg
    70441 ttgatcttcg ctttgatatt acggtcagca gagcaggaga cgcccatacc gaccgggcag
    70501 gatgcgccgt gacgtggcag gcgaatcacg cggatgtcgt gagcgaagta tttaccacca
    70561 aactgcgcac ccagaccaag attttgcgct tcgatcagca attctttttc cagttccaca
    70621 tcgcggaacg cctgaccgtg ctcattccct tccgttggca gttcatcata gtatttcgcg
    70681 gaagccagtt tcaccgtttt aaggttcgtt tctgcagaag ttccaccaat aacgaacgca
    70741 atatgatacg gaggacaggc cgccgtaccc agcgtgcgca tcttctcaac caggtaattt
    70801 ttcagtttcc ccggcgtcag taacgctttg gtttcctgat agagatacgt cttgtttgcc
    70861 gaaccaccac ctttggcgat acagaggaat ttgtattcgt cgccatcaac ggcataaaga
    70921 tcgatctgcg ctggcagatt ggtgccggta ttcacttctt tatacatatc cagcggcgcg
    70981 ttttgcgagt agcgcagatt atcttcgata taagtgttat agacaccgcg cgccagcgcc
    71041 gcttcatcac caccaccggt ccatacacgc tgcccttttt taccaacaat aatcgcggtg
    71101 ccggtatcct gacaggttgg cagaacgcct ttcgccgcga tgtcggagtt acgcaggaat
    71161 tgcagcgcca catatttatc attttcgctg gcctccgggt cacgcagaat gtcggccacc
    71221 tgttgttggt gcgccggacg cagcatgaac gacgcatcat gaaacgcctg gcgcgccaac
    71281 agagttaacg cttcgggtgc gactttcaaa atctcctgcc cttcaaattc agatacgcta
    71341 acgtgttcgc tggttagcag gtaatactca gtatcatctt ttttgagtgg aaaaggagcc
    71401 tgataatgaa agggtttgtt tgacattgtt ctctcactta ctgcctggtt tggttatgct
    71461 ctgggcgggt gttccgttgc cctgttaaaa gcgagtaaca atatcctaca cactttttta
    71521 acaaaaactg agactagtac gactttttgc ggctccaggt tacttcccgt aggattcttg
    71581 ctttaatagt gggattaatt tccacattaa aacagggatt gatcatgcaa aaactcatta
    71641 actcagtgca aaactatgcc tggggcagca aaacggcgtt gactgaactt tatggtatgg
    71701 aaaatccgtc cagccagccg atggccgagc tgtggatggg cgcacatccg aaaagcagtt
    71761 cacgagtgca gaatgccgcc ggagatatcg tttcactgcg tgatgtgatt gagagtgata
    71821 aatcgactct gctcggagag gccgttgcca aacgctttgg cgaactgcct ttcctgttca
    71881 aagtattatg cgcagcacag ccactctcca ttcaggttca tccaaacaaa cacaattctg
    71941 aaatcggttt tgccaaagaa aatgccgcag gtatcccgat ggatgccgcc gagcgtaact
    72001 ataaagatcc taaccacaag ccggagctgg tttttgcgct gacgcctttc cttgcgatga
    72061 acgcgtttcg tgaattttcc gagattgtct ccctactcca gccggtcgca ggtgcacatc
    72121 cggcgattgc tcacttttta caacagcctg atgccgaacg tttaagcgaa ctgttcgcca
    72181 gcctgttgaa tatgcagggt gaagaaaaat cccgcgcgct ggcgatttta aaatcggccc
    72241 tcgatagcca gcagggtgaa ccgtggcaaa cgattcgttt aatttctgaa ttttacccgg
    72301 aagacagcgg tctgttctcc ccgctattgc tgaatgtggt gaaattgaac cctggcgaag
    72361 cgatgttcct gttcgctgaa acaccgcacg cttacctgca aggcgtggcg ctggaagtga
    72421 tggcaaactc cgataacgtg ctgcgtgcgg gtctgacgcc taaatacatt gatattccgg
    72481 aactggttgc caatgtgaaa ttcgaagcca aaccggctaa ccagttgttg acccagccgg
    72541 tgaaacaagg tgcagaactg gacttcccga ttccagtgga tgattttgcc ttctcgctgc
    72601 atgaccttag tgataaagaa accaccatta gccagcagag tgccgccatt ttgttctgcg
    72661 tcgaaggcga tgcaacgttg tggaaaggtt ctcagcagtt acagcttaaa ccgggtgaat
    72721 cagcgtttat tgccgccaac gaatcaccgg tgactgtcaa aggccacggc cgtttagcgc
    72781 gtgtttacaa caagctgtaa gagcttactg aaaaaattaa catctcttgc taagcttatt
    72841 aaaggcttat aacaccttca ggcggccagt ccgcctgatt tcattttatg gataatcatt
    72901 atgaataaat cgctggtagc ggtaggcgtc attgttgcgc taggcgtagt ctggacaggc
    72961 ggcgcatggt atacaggcaa gaagattgaa acccatctcg aagacatggt cgcgcaggcg
    73021 aacgcgcaac tcaaactgac agctcctgaa tccaacctgg aagtgagtta tcaaaactat
    73081 catcgcggcg tattcagcag ccagttgcaa ctgttggtga aacccattgc cgggaaagaa
    73141 aatccgtgga ttaaaagcgg tcagagcgtc atcttcaacg aatcggttga tcatggtccc
    73201 ttcccgcttg cccagcttaa aaaactgaac ctgatcccgt cgatggcatc aattcaaacc
    73261 acgctggtta ataacgaagt aagcaaacca ctgtttgata tggcaaaagg tgaaacgcct
    73321 tttgagatta actcgcgcat tggttacagc ggtgattcca gttccgatat ttcgctcaag
    73381 ccactgaatt acgagcaaaa ggatgaaaaa gtcgccttta gcggcggcga gttccagtta
    73441 aatgctgaca gagacggcaa agccatctcc ctttccgggg aggcgcaaag tggtcggata
    73501 gacgcagtta acgaatacaa ccagaaagtg cagttgacct ttaataatct gaaaaccgac
    73561 ggttccagca cgctggcaag ttttggtgag cgtgtaggaa atcaaaaact gtcactggaa
    73621 aaaatgacca tttcagtgga aggcaaagaa ctggcactgc tggaaggcat ggagatcagc
    73681 ggtaaatcgg atctggtcaa tgacggtaaa acgatcaata gccaactgga ttactcgcta
    73741 aacagcctga aggtacagaa tcaggatctg ggcagcggca agctgacttt aaaagtcggc
    73801 cagattgatg gtgaagcctg gcatcagttt agccagcaat ataacgcgca aactcaggcg
    73861 ctgctggcac agccagaaat tgccaacaac cccgaacttt atcaggagaa agtgacggaa
    73921 gccttcttta gcgccctgcc gctgatgctg aaaggcgatc cggtgattac tatcgcgccg
    73981 ctaagctgga aaaacagtca gggtgaaagt gccctgaatc tgtcgctgtt cctgaaagat
    74041 ccggcaacga ctaaagaagc gccgcaaacg ctggcgcagg aagtagatcg ttcggttaaa
    74101 tctctggatg cgaaactgac cattccggtg gatatggcaa ctgagtttat gactcaggta
    74161 gcgaagctgg aaggttatca ggaagatcaa gcgaaaaaac tggcgaaaca gcaagttgaa
    74221 ggtgcatcag caatggggca gatgttccgt ctgaccacct tgcaggacaa taccatcacc
    74281 accagcctgc aatatgctaa cggtcagata acgttaaatg ggcagaaaat gtcgttagaa
    74341 gattttgttg gcatgtttgc gatgccagct cttaacgttc cggctgtacc ggcaattccg
    74401 cagcagtaat tcacggccaa aacgccggag aattttcact ccggcgtttt catctgaaaa
    74461 caaaccagag attgcctgat gcgctacgct tatcaggcct accttttcct gcaacacttt
    74521 gaatttatga gtttttgtag gctggataag gcgtttacgc cgcatccggc ataaaaaacg
    74581 cgcactttgt caacaatctg aaacgccgga gatttttctc tccggcgtta ttttttactt
    74641 cagcataaag tcatactgaa tactcatttt cacatcccag taattcaggc gatcgcccag
    74701 cgtactgcca tcgcgctttt cccgcccttt atcggcagca agaaagaaat tcatcccttg
    74761 taacggccct tcgctgaccc gatagttcat accacctaag atacccgtgc cttcaatccg
    74821 cacatctcgt ttactggatt catagccgcg agtcacaatc ggtgcaaatg tcagcgtaaa
    74881 ttgcggcgtt aaacgatagt tgacgcccaa ttgccaggac tgcattcctt cacggttacg
    74941 atcaagactt aatgaaaagg gatagccaat gtcggtatcc accacggcac cattcattgc
    75001 atggcgcaaa taaccaccac tgccaaatac acttccccac ggcgcattcc aggtcaattg
    75061 tccactaacc aacgccgttt cattgggctg gctggtatta cgacttaaac cttcgagctg
    75121 cgcgtaaaag ccgagcaact cccctttcaa cgtataattc gggtcgagcg gtatgctttt
    75181 ctcgagaata ctttgtatct gattggaaac atctttctgt accgatgccc ccagagaata
    75241 gcgactggta tcatcatgcc aactggcggc taaaaaagaa cgtgcgcgat gtttatcgta
    75301 ttttcccgtc tggctgttat acaccccttc atcacgaata ccggttgttg aatcagtcca
    75361 taaaaatact cgtgaacgcc acattcccca cagatcaata tttggtgtta aagcccaata
    75421 acctaatgct ccttcagaaa ctgtcggtaa tgtacgttgc gaactgccag ccattaacgg
    75481 aatacgataa ggtaagctac cataactata atccgtcccg aagcgaccta atttaagcag
    75541 gctattttca ctgggtttta tttttactgc ccccagggca aagccaaaac ttttatcacc
    75601 atcaagatac caccgggtac tcatatcagc acgagcacct aatttataaa cataatacgc
    75661 cccgccttca acgccaataa tattggaata ataaccacta ttgaaatcga gcaatcctcc
    75721 ctgtacccat gcgtaaatat ctcttcccgc gccagcactg ggcttatccg ccctgcgcaa
    75781 ttcgtttttc agtctgatgc gtagtcccgc ttcatcgtca gccagttgcg ccgcataagc
    75841 agaggtaagg ccagaggcag ccgtcaggca aataacggcc atggccacta tttttctcat
    75901 agttgcacct ttgatgttct gattattttt attgaatatt aattagtgat atcgctgatt
    75961 aattgctgct gcactttttt acgattatca atttcaacca cgatttcttt gaatttttta
    76021 tccgtgagcg gataaaacca gataataacg aatgccagta gcataaatcc gcaaggtact
    76081 aaggcaattg atgtgcggat gcccataata acttccggcg tttgcacctg attggcgata
    76141 tatccgctta accccaaaat aaaggcagga attgaacctc cgattgcctg accgcattta
    76201 cgggtaaatg agaatagtga ataggtgagc ccttcaattc gcacgccggt caggtattca
    76261 ccgtattcta cggtatcagc ttccagcgcc cacatcacgg tcatggtaac gccctgacca
    76321 attgaagcga tggccaacgc aaccaacgcc accggcagtg accagacgga aacccagaag
    76381 aacagcagat aaccgcaggt tcccagcaaa gcgccaatca ggaaggtatt ctttttaccg
    76441 atcctcgcga ccatccccgg caccagcggt gccgatgcca cagtaccaac caggttttgc
    76501 accagtacca gcacagtgaa cagcccggta tcatttaaca catagcgcac gtagaacaac
    76561 gacgaggcgc tgaccgcaaa ggtcgaaatc agcacacaca gcgcaccgat gcacaacata
    76621 aacagcgggc gattccgttt cagggtttgc agactgatat tcaatgacgg ctgcgcaacg
    76681 atacgtacca cattctcacg cgtcgatttg aagcagatga agtaaagcac cattccggca
    76741 atcgccagca caattgtcca gaaatggtat accgacacca tctcttccgg gctggagttc
    76801 ttaatgctcg gtcctatcag aaatgccagg cagacaaagg tcaatgaagc ggcaatccca
    76861 cgagccgcgc ccagacgggc gcgggattgt ggttgttggg tcatcgcggt agcaagtgaa
    76921 ccataaggaa tattcaccag gctgtagcaa agcccgaggc ccatgtaggt caaatatgca
    76981 tacaccactt tgctaccatg gctccagtcg gtcggcaccc agaataccag cacgctgaag
    77041 atcattaacg gcgcagtacc gaagagtaaa aacgggcgga attttcccca gcgggtattc
    77101 acactgtcca ccactcgtcc ggcaaagacg tcggcgaagg catcgaatac ccgcaccagt
    77161 aacagcatgg tgcccgccgc agcggcaccg acgccagcga cgtcggtgta gtaactcaac
    77221 aggaagagcg cccccattgc gaaggcgaag ttattggcga cgtcaccgag gctgtagccg
    77281 acgatggtgc gccaggagag ttgttgattc attgtttgcc tccctgctgc ggtttttcac
    77341 cgaagttcat gccagtccag cgtttttgca gcagaaaagc cgccgacttc ggtttgcggt
    77401 cgcgagtgaa gatccctttc ttgttaccgc caacgcgcaa tatgccttgc gaggtcgcaa
    77461 aatcggcgaa attccatacc tgttcaccga cgacggcgct gacgcgatca aagacgcggt
    77521 gatacatatc cagccatgca cactgatact cttcactcca catgtcggtg tacattgagt
    77581 gcagcccggc taacgtatcc acgccgtatt cggtgatgat aatcggctga tgcagtttct
    77641 cctgccaggc cagaagttct ttttccagta ccttctctgc cgtttccaaa tcgccgcttt
    77701 ggacatacca tccgtaataa cggttcaggc acagcacatc aaagagatcg ctgatggtat
    77761 cggtgtgagc gtcgcagaac attacattga cgcaggtgat cggacgcgtc gggtcgagtt
    77821 tacgcgttgc ttccgccagt ggcgcgaaat attcccgtgc accttgcgga cgggtatccg
    77881 gttcgttggc aatactccac atcaccacgc ttgggtggtt tttgtcacgc gctatcagct
    77941 ctttaatcgc ctgtaagtgc gcttgctgag tttccccgtt gactgcctct tcgctgtaca
    78001 gttctttcgg cttgttgccc gcttcgaaac caatgcctaa agagaggtta aagccgacag
    78061 cagcagtttc atcaatcacc acgatgccat gttcatctgc ccagtcgagc atctcttcag
    78121 cgtaagggta atgcgaggta cggtaggagt tggccccaat ccagtccatt aatgcgtggt
    78181 cgtgcaccat cagcacgtta tcgaatcctt tgccacgcaa gtccgcatct tcatgacgac
    78241 caaagccagt aaagtagaac ggtttgtggt taatcaggaa ctgttcgccc ttcactgcca
    78301 ctgaccggat gccgacgcga agcgggtaga tatcacactc tgtctggctt ttggctgtga
    78361 cgcacagttc atagagataa ccttcacccg gttgccagag gtgcggattc accacttgca
    78421 aagtcccgct agtgccttgt ccagttgcaa ccacctgttg atccgcatca cgcagttcaa
    78481 cgctgacatc accattggcc accacctgcc agtcaacaga cgcgtggtta cagtcttgcg
    78541 cgacatgcgt caccacggtg atatcgtcca cccaggtgtt cggcgtggtg tagagcatta
    78601 cgctgcgatg gatcccggca tagttaaaga aatcatggaa gtaagactgc tttttcttgc
    78661 cgttttcgtc ggtaatcacc attcccggcg ggatagtctg ccagttcagt tcgttgttca
    78721 cacaaacggt gatacgtaca cttttcccgg caataacata cggcgtgaca tcggcttcaa
    78781 atggcgtata gccgccctga tgctccatca cttcctgatt attgacccac actttgccgt
    78841 aatgagtgac cgcatcgaaa cgcagcacga tacgctggcc tgcccaacct ttcggtataa
    78901 agacttcgcg ctgataccag acgttgcccg cataattacg aatatctgca tcggcgaact
    78961 gatcgttaaa actgcctggc acagcaattg cccggctttc ttgtaacgcg ctttcccacc
    79021 aacgctgatc aattccacag ttttcgcgat ccagactgaa tgcccacagg ccgtcgagtt
    79081 ttttgatttc acgggttggg gtttctacag gacgtaacat aagggactcc tcattaagat
    79141 aataatactg gtcaaccttt aatctgatta gttaaccaat tctgtgttgc acacagttat
    79201 ctgtgagaaa cccatggaag agaaatggaa ataaaaagaa ctgacaccgc gatcaaaaaa
    79261 ccaaaaactg caatacccac agcgtgatat agatcgcatt aatctttaaa acagcgttga
    79321 cagaagccat aaaataagca accgaacgtt cgttcgagcg tattgggaac gataaactct
    79381 catgacatat ggctacagtg aatattttgg ggggaattct ccgaagccgg gaaggataat
    79441 ttttgcacag agaaattacg atatgaaacg cgggcacagt tatcaccagc ccgccgaaaa
    79501 agagagagaa tcaggatgcg gttaagatac cgccaatcat cgcgcgtaat ccctgagcaa
    79561 acgacgcttc acgcgccagg tcttccgctt cgatatcggc cagtacaccg taggtcagat
    79621 ccagcagtaa acgtgaagtc attgccgtgt tgatgtctgg gcttatctcg cctttttgct
    79681 gggcatcacg catccgctgg gcaacaaatt ccgtgatcgt catatgctta tttttaagca
    79741 tggcggcgac ctgtgggtta cgcatccctt ccgccataat ttcaaccacc agcgcccgtt
    79801 gtccaaaggc ttcatgggtg agagagacaa tggactcgac catatagtca acgaaatgaa
    79861 tcccttcaat cggttcccgg aaacgggcca gcgccctctc ctggtcctgt aagataatcg
    79921 cctgaatcaa ggcttctttg gagatgaaat ggtgatagag cgtcccggga ctaatggcgc
    79981 aagatttaca gatggctttc atcgaggcac tgtgaaatcc attttctgaa aaaatctctc
    80041 tggcagcatt gaggatccgg gtccgtgtcg gttgtgcttc agtctgcatg ttatccatca
    80101 ttaaaccatt atagttagcg tgaataacta gtttaccgcg ttgtcaggtg agcgcctatc
    80161 attgctgttg aatagtgcga aggcacactc tattcgtata cttttcactc tgaaattggt
    80221 taaccaatag ccgtgatttg gctgccagtt atttagcaga gacgttgatg agtttatccc
    80281 ggtaaacaac acgcacggtt gtttaccggt ccgttagtgt attaattgag ctcctgtacc
    80341 ccaccaccgg agacggtgag aatttgtccg cttacccagc tcgcagcagg cgagcaaagg
    80401 aacagcgctg cgttagcaat atcttgcggt tggcccagac gtctgatcgg cgtgtgctgt
    80461 aacatttttt gttcaatttc tggtgtaata acggatttca gggcatcggt taatattgcc
    80521 cccggcgcaa tgccatttac ccgaatattt ttttcaccca ggtcaaacgc catatttctg
    80581 accagatgac tggccgcagc
//
LOCUS       DBBGLQ010000014        78201 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.014, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000014 DBBGLQ010000000
VERSION     DBBGLQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.014"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..334
                     /locus_tag="ACIYSB_001222"
     CDS             <1..334
                     /locus_tag="ACIYSB_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HGC5428305.1"
                     /translation="HRPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKA
                     GNNGPWVTHWEEMAKKTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEY
                     SVIDNSEE"
     gene            391..585
                     /gene="ralR"
                     /locus_tag="ACIYSB_001223"
     CDS             391..585
                     /gene="ralR"
                     /locus_tag="ACIYSB_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HGC5428306.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSKK
                     EALERWNKRTTGNNNGGVHV"
     gene            578..766
                     /locus_tag="ACIYSB_001224"
     CDS             578..766
                     /locus_tag="ACIYSB_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HGC5428307.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            866..1081
                     /gene="xisR"
                     /locus_tag="ACIYSB_001225"
     CDS             866..1081
                     /gene="xisR"
                     /locus_tag="ACIYSB_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HGC5428308.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            1083..2318
                     /gene="intR"
                     /locus_tag="ACIYSB_001226"
     CDS             1083..2318
                     /gene="intR"
                     /locus_tag="ACIYSB_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5428309.1"
                     /translation="MSKLPTGVEIRGRNIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISYIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRMCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            2370..3305
                     /gene="ttcA"
                     /locus_tag="ACIYSB_001227"
     CDS             2370..3305
                     /gene="ttcA"
                     /locus_tag="ACIYSB_001227"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGC5428310.1"
                     /translation="MKENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3434..4807)
                     /gene="dbpA"
                     /locus_tag="ACIYSB_001228"
     CDS             complement(3434..4807)
                     /gene="dbpA"
                     /locus_tag="ACIYSB_001228"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGC5428311.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4837..5010)
                     /gene="ynaL"
                     /locus_tag="ACIYSB_001229"
     CDS             complement(4837..5010)
                     /gene="ynaL"
                     /locus_tag="ACIYSB_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGC5428312.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(5285..6268)
                     /gene="zntB"
                     /locus_tag="ACIYSB_001230"
     CDS             complement(5285..6268)
                     /gene="zntB"
                     /locus_tag="ACIYSB_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGC5428313.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(6282..6329)
                     /gene="ydaN"
                     /locus_tag="ACIYSB_001231"
     CDS             complement(6282..6329)
                     /gene="ydaN"
                     /locus_tag="ACIYSB_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGC5428314.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6523..7755
                     /gene="dgcM"
                     /locus_tag="ACIYSB_001232"
     CDS             6523..7755
                     /gene="dgcM"
                     /locus_tag="ACIYSB_001232"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGC5428315.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7776..8339)
                     /gene="smrA"
                     /locus_tag="ACIYSB_001233"
     CDS             complement(7776..8339)
                     /gene="smrA"
                     /locus_tag="ACIYSB_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGC5428316.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8669..9577)
                     /gene="abgR"
                     /locus_tag="ACIYSB_001234"
     CDS             complement(8669..9577)
                     /gene="abgR"
                     /locus_tag="ACIYSB_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5428317.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELVFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9753..11063
                     /gene="abgA"
                     /locus_tag="ACIYSB_001235"
     CDS             9753..11063
                     /gene="abgA"
                     /locus_tag="ACIYSB_001235"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGC5428318.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTSLNFPWTRGI"
     gene            11063..12508
                     /gene="abgB"
                     /locus_tag="ACIYSB_001236"
     CDS             11063..12508
                     /gene="abgB"
                     /locus_tag="ACIYSB_001236"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGC5428319.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGITPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQSFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECLQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12545..14071
                     /gene="abgT"
                     /locus_tag="ACIYSB_001237"
     CDS             12545..14071
                     /gene="abgT"
                     /locus_tag="ACIYSB_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGC5428320.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            14082..14597
                     /gene="ogt"
                     /locus_tag="ACIYSB_001238"
     CDS             14082..14597
                     /gene="ogt"
                     /locus_tag="ACIYSB_001238"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGC5428321.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLNI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14792..15544
                     /gene="fnr"
                     /locus_tag="ACIYSB_001239"
     CDS             14792..15544
                     /gene="fnr"
                     /locus_tag="ACIYSB_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGC5428322.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15696..16646
                     /gene="uspE"
                     /locus_tag="ACIYSB_001240"
     CDS             15696..16646
                     /gene="uspE"
                     /locus_tag="ACIYSB_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGC5428323.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16696..16953)
                     /gene="ynaJ"
                     /locus_tag="ACIYSB_001241"
     CDS             complement(16696..16953)
                     /gene="ynaJ"
                     /locus_tag="ACIYSB_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGC5428324.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            17197..18228
                     /gene="ynaI"
                     /locus_tag="ACIYSB_001242"
     CDS             17197..18228
                     /gene="ynaI"
                     /locus_tag="ACIYSB_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGC5428325.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(18279..19892)
                     /gene="mppA"
                     /locus_tag="ACIYSB_001243"
     CDS             complement(18279..19892)
                     /gene="mppA"
                     /locus_tag="ACIYSB_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGC5428326.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(20229..21128)
                     /gene="pgrR"
                     /locus_tag="ACIYSB_001244"
     CDS             complement(20229..21128)
                     /gene="pgrR"
                     /locus_tag="ACIYSB_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGC5428327.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            21266..22186
                     /gene="ycjY"
                     /locus_tag="ACIYSB_001245"
     CDS             21266..22186
                     /gene="ycjY"
                     /locus_tag="ACIYSB_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGC5428328.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            22257..22394
                     /locus_tag="ACIYSB_001246"
                     /pseudo
     CDS             22257..22394
                     /locus_tag="ACIYSB_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            22521..23249
                     /gene="mpaA"
                     /locus_tag="ACIYSB_001247"
     CDS             22521..23249
                     /gene="mpaA"
                     /locus_tag="ACIYSB_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGC5428329.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCANPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(23224..24189)
                     /gene="ycjG"
                     /locus_tag="ACIYSB_001248"
     CDS             complement(23224..24189)
                     /gene="ycjG"
                     /locus_tag="ACIYSB_001248"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGC5428330.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLASRR
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            24308..24814
                     /gene="tpx"
                     /locus_tag="ACIYSB_001249"
     CDS             24308..24814
                     /gene="tpx"
                     /locus_tag="ACIYSB_001249"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGC5428331.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(24858..26399)
                     /gene="tyrR"
                     /locus_tag="ACIYSB_001250"
     CDS             complement(24858..26399)
                     /gene="tyrR"
                     /locus_tag="ACIYSB_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGC5428332.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(26547..27608)
                     /gene="ycjF"
                     /locus_tag="ACIYSB_001251"
     CDS             complement(26547..27608)
                     /gene="ycjF"
                     /locus_tag="ACIYSB_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGC5428333.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(27605..29002)
                     /gene="ycjX"
                     /locus_tag="ACIYSB_001252"
     CDS             complement(27605..29002)
                     /gene="ycjX"
                     /locus_tag="ACIYSB_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGC5428334.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMSEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            29158..30156
                     /gene="ycjW"
                     /locus_tag="ACIYSB_001253"
     CDS             29158..30156
                     /gene="ycjW"
                     /locus_tag="ACIYSB_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGC5428335.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALKAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(30267..31172)
                     /gene="ompG"
                     /locus_tag="ACIYSB_001254"
     CDS             complement(30267..31172)
                     /gene="ompG"
                     /locus_tag="ACIYSB_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGC5428336.1"
                     /translation="MKKLLPCTALVICAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(31217..32299)
                     /locus_tag="ACIYSB_001255"
     CDS             complement(31217..32299)
                     /locus_tag="ACIYSB_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5428337.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVSGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(32313..32972)
                     /gene="pgmB"
                     /locus_tag="ACIYSB_001256"
     CDS             complement(32313..32972)
                     /gene="pgmB"
                     /locus_tag="ACIYSB_001256"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGC5428338.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(32969..35236)
                     /gene="ycjT"
                     /locus_tag="ACIYSB_001257"
     CDS             complement(32969..35236)
                     /gene="ycjT"
                     /locus_tag="ACIYSB_001257"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGC5428339.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGSGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGSYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETMTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAYTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLITVAEESVFCLGDF
                     ILPFNGTATKHQEDE"
     gene            complement(35233..36288)
                     /gene="ycjS"
                     /locus_tag="ACIYSB_001258"
     CDS             complement(35233..36288)
                     /gene="ycjS"
                     /locus_tag="ACIYSB_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGC5428340.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPGQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(36298..37086)
                     /gene="ycjR"
                     /locus_tag="ACIYSB_001259"
     CDS             complement(36298..37086)
                     /gene="ycjR"
                     /locus_tag="ACIYSB_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGC5428341.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(37105..38157)
                     /gene="ycjQ"
                     /locus_tag="ACIYSB_001260"
     CDS             complement(37105..38157)
                     /gene="ycjQ"
                     /locus_tag="ACIYSB_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGC5428342.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(38188..39030)
                     /gene="ycjP"
                     /locus_tag="ACIYSB_001261"
     CDS             complement(38188..39030)
                     /gene="ycjP"
                     /locus_tag="ACIYSB_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGC5428343.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTAYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(39017..39898)
                     /gene="ycjO"
                     /locus_tag="ACIYSB_001262"
     CDS             complement(39017..39898)
                     /gene="ycjO"
                     /locus_tag="ACIYSB_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGC5428344.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(39919..41211)
                     /gene="ycjN"
                     /locus_tag="ACIYSB_001263"
     CDS             complement(39919..41211)
                     /gene="ycjN"
                     /locus_tag="ACIYSB_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGC5428345.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQASQKKLDELIEQH"
     gene            complement(41225..42904)
                     /gene="ycjM"
                     /locus_tag="ACIYSB_001264"
     CDS             complement(41225..42904)
                     /gene="ycjM"
                     /locus_tag="ACIYSB_001264"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HGC5428346.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(43117..43431)
                     /gene="pspE"
                     /locus_tag="ACIYSB_001265"
     CDS             complement(43117..43431)
                     /gene="pspE"
                     /locus_tag="ACIYSB_001265"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGC5428347.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(43506..43727)
                     /gene="pspD"
                     /locus_tag="ACIYSB_001266"
     CDS             complement(43506..43727)
                     /gene="pspD"
                     /locus_tag="ACIYSB_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGC5428348.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(43736..44095)
                     /gene="pspC"
                     /locus_tag="ACIYSB_001267"
     CDS             complement(43736..44095)
                     /gene="pspC"
                     /locus_tag="ACIYSB_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGC5428349.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(44095..44319)
                     /gene="pspB"
                     /locus_tag="ACIYSB_001268"
     CDS             complement(44095..44319)
                     /gene="pspB"
                     /locus_tag="ACIYSB_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGC5428350.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(44373..45041)
                     /gene="pspA"
                     /locus_tag="ACIYSB_001269"
     CDS             complement(44373..45041)
                     /gene="pspA"
                     /locus_tag="ACIYSB_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGC5428351.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            45208..46185
                     /gene="pspF"
                     /locus_tag="ACIYSB_001270"
     CDS             45208..46185
                     /gene="pspF"
                     /locus_tag="ACIYSB_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGC5428352.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(46305..47570)
                     /gene="puuE"
                     /locus_tag="ACIYSB_001271"
     CDS             complement(46305..47570)
                     /gene="puuE"
                     /locus_tag="ACIYSB_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGC5428353.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(47608..48888)
                     /gene="puuB"
                     /locus_tag="ACIYSB_001272"
     CDS             complement(47608..48888)
                     /gene="puuB"
                     /locus_tag="ACIYSB_001272"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGC5428354.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(48890..50377)
                     /gene="puuC"
                     /locus_tag="ACIYSB_001273"
     CDS             complement(48890..50377)
                     /gene="puuC"
                     /locus_tag="ACIYSB_001273"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGC5428355.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(50652..51209)
                     /gene="puuR"
                     /locus_tag="ACIYSB_001274"
     CDS             complement(50652..51209)
                     /gene="puuR"
                     /locus_tag="ACIYSB_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGC5428356.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(51236..52000)
                     /gene="puuD"
                     /locus_tag="ACIYSB_001275"
     CDS             complement(51236..52000)
                     /gene="puuD"
                     /locus_tag="ACIYSB_001275"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGC5428357.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            52212..53630
                     /gene="puuA"
                     /locus_tag="ACIYSB_001276"
     CDS             52212..53630
                     /gene="puuA"
                     /locus_tag="ACIYSB_001276"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGC5428358.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            53779..53943
                     /locus_tag="ACIYSB_001277"
     CDS             53779..53943
                     /locus_tag="ACIYSB_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428359.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            53933..55318
                     /gene="puuP"
                     /locus_tag="ACIYSB_001278"
     CDS             53933..55318
                     /gene="puuP"
                     /locus_tag="ACIYSB_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGC5428360.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            55452..55697
                     /gene="ymjA"
                     /locus_tag="ACIYSB_001279"
     CDS             55452..55697
                     /gene="ymjA"
                     /locus_tag="ACIYSB_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HGC5428361.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            56010..57653
                     /gene="sapA"
                     /locus_tag="ACIYSB_001280"
     CDS             56010..57653
                     /gene="sapA"
                     /locus_tag="ACIYSB_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGC5428362.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            57650..58615
                     /gene="sapB"
                     /locus_tag="ACIYSB_001281"
     CDS             57650..58615
                     /gene="sapB"
                     /locus_tag="ACIYSB_001281"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGC5428363.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            58602..59492
                     /gene="sapC"
                     /locus_tag="ACIYSB_001282"
     CDS             58602..59492
                     /gene="sapC"
                     /locus_tag="ACIYSB_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGC5428364.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            59492..60484
                     /gene="sapD"
                     /locus_tag="ACIYSB_001283"
     CDS             59492..60484
                     /gene="sapD"
                     /locus_tag="ACIYSB_001283"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGC5428365.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            60486..61292
                     /gene="sapF"
                     /locus_tag="ACIYSB_001284"
     CDS             60486..61292
                     /gene="sapF"
                     /locus_tag="ACIYSB_001284"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGC5428366.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            61360..61713
                     /gene="ycjD"
                     /locus_tag="ACIYSB_001285"
     CDS             61360..61713
                     /gene="ycjD"
                     /locus_tag="ACIYSB_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HGC5428367.1"
                     /translation="MIDKIKSNARDLRRNLTLQERKLWRYLRSRRFSDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            62081..62869
                     /gene="fabI"
                     /locus_tag="ACIYSB_001286"
     CDS             62081..62869
                     /gene="fabI"
                     /locus_tag="ACIYSB_001286"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGC5428368.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            63013..64140
                     /gene="yciW"
                     /locus_tag="ACIYSB_001287"
     CDS             63013..64140
                     /gene="yciW"
                     /locus_tag="ACIYSB_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGC5428369.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVDLFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIISQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLEKLLNSTRGNTQPDNQLAALLCARIN
                     GSPACFATCMDSSNEYKKISTLMRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            64208..66142
                     /gene="rnb"
                     /locus_tag="ACIYSB_001288"
     CDS             64208..66142
                     /gene="rnb"
                     /locus_tag="ACIYSB_001288"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGC5428370.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(66220..66324)
                     /locus_tag="ACIYSB_001289"
     CDS             complement(66220..66324)
                     /locus_tag="ACIYSB_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428371.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            66378..68363
                     /gene="pdeR"
                     /locus_tag="ACIYSB_001290"
     CDS             66378..68363
                     /gene="pdeR"
                     /locus_tag="ACIYSB_001290"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGC5428372.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(68345..68473)
                     /gene="ymiD"
                     /locus_tag="ACIYSB_001291"
     CDS             complement(68345..68473)
                     /gene="ymiD"
                     /locus_tag="ACIYSB_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGC5428373.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            68511..68684
                     /gene="yciZ"
                     /locus_tag="ACIYSB_001292"
     CDS             68511..68684
                     /gene="yciZ"
                     /locus_tag="ACIYSB_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGC5428374.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            68774..69523
                     /gene="yciT"
                     /locus_tag="ACIYSB_001293"
     CDS             68774..69523
                     /gene="yciT"
                     /locus_tag="ACIYSB_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGC5428375.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            69792..70010
                     /gene="osmB"
                     /locus_tag="ACIYSB_001294"
     CDS             69792..70010
                     /gene="osmB"
                     /locus_tag="ACIYSB_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGC5428376.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(70136..70462)
                     /gene="yciH"
                     /locus_tag="ACIYSB_001295"
     CDS             complement(70136..70462)
                     /gene="yciH"
                     /locus_tag="ACIYSB_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGC5428377.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(70462..71199)
                     /gene="pyrF"
                     /locus_tag="ACIYSB_001296"
     CDS             complement(70462..71199)
                     /gene="pyrF"
                     /locus_tag="ACIYSB_001296"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGC5428378.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(71393..72562)
                     /gene="lapB"
                     /locus_tag="ACIYSB_001297"
     CDS             complement(71393..72562)
                     /gene="lapB"
                     /locus_tag="ACIYSB_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGC5428379.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(72569..72877)
                     /gene="lapA"
                     /locus_tag="ACIYSB_001298"
     CDS             complement(72569..72877)
                     /gene="lapA"
                     /locus_tag="ACIYSB_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGC5428380.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(73026..73790)
                     /gene="pgpB"
                     /locus_tag="ACIYSB_001299"
     CDS             complement(73026..73790)
                     /gene="pgpB"
                     /locus_tag="ACIYSB_001299"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGC5428381.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            73960..74550
                     /gene="ribA"
                     /locus_tag="ACIYSB_001300"
     CDS             73960..74550
                     /gene="ribA"
                     /locus_tag="ACIYSB_001300"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGC5428382.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(74614..77289)
                     /gene="acnA"
                     /locus_tag="ACIYSB_001301"
     CDS             complement(74614..77289)
                     /gene="acnA"
                     /locus_tag="ACIYSB_001301"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGC5428383.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKGIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(77453..77548)
                     /gene="ymiC"
                     /locus_tag="ACIYSB_001302"
     CDS             complement(77453..77548)
                     /gene="ymiC"
                     /locus_tag="ACIYSB_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGC5428384.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(77662..77829)
                     /gene="yciX"
                     /locus_tag="ACIYSB_001303"
     CDS             complement(77662..77829)
                     /gene="yciX"
                     /locus_tag="ACIYSB_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGC5428385.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(77832..77972)
                     /gene="ymiA"
                     /locus_tag="ACIYSB_001304"
     CDS             complement(77832..77972)
                     /gene="ymiA"
                     /locus_tag="ACIYSB_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGC5428386.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        19236 a        20288 c        19248 g        19429 t
ORIGIN      
        1 acaccgcccg ggagaaaacg aagatgcccc agttacccac gtctatgctg tcgcaagact
       61 gaaagacgga ggtactcagt ttgaagttat gacgcgcaaa cagattgagc tggtgcgcag
      121 ccaaagtaaa gctggtaata acgggccgtg ggtaactcac tgggaagaaa tggcaaagaa
      181 aacggctatt cgtcgcctgt tcaaatacct gcccgtatca attgagatcc agcgcgcggt
      241 atcaatggat gaaaaggaac cactgacaat cgatccggca gattcctctg tattaaccgg
      301 ggaatacagt gtaatcgata attcagagga ataattcagc taggcggtgt aatgcaccgc
      361 caacgtgaaa tattttttat gagaacaatt atgagatatg acgatgttaa accatgtccg
      421 ttttgtggtt gtccatcagt aacggtgaaa gccatttcag gatattaccg cgcgaagtgt
      481 aacggatgcg aatcccgaac cggctatggt ggaagtaaaa aagaagcact cgaaagatgg
      541 aataaacgaa ccactggaaa taataatgga ggtgttcatg tataaaatta ccgccactat
      601 tgaaaaggaa ggtggcactc ctactaactg gacaagatat tcaaaatcta aactaacgaa
      661 atcagaatgc gaaaaaatgc tctcaggtaa aaaagaagca ggcgtttcca gagagcagaa
      721 agtaaaactg ataaatttta attgcgagaa acttcagtcc tcgtgaattg cattgtattc
      781 aaattaaaac ttcatagctg attattaata atcaacatcg ggcgtcaatt tcagtctaac
      841 attggcgcct gccagaggtg atgcgatggc acaagtaatc tttaatgaag agtggatggt
      901 tgaatacggc ctgatgcttc gcactggtct gggggccaga caaattgaag cataccgcca
      961 gaactgttgg gtggagggct tccacttcaa acgagtatct cctttaggta agccagacag
     1021 caaacgaggg attatctggt acaactatcc aaagataaat cagtttatca aagactcatg
     1081 atatgtctaa attaccaaca ggtgtcgaga ttagaggtag aaacattcgc atctggttca
     1141 tgtttcgagg aaaacgatgt cgggaaacat taaaaggctg ggagattaca aacagtaata
     1201 ttaaaaaggc cggaaattta agagcgctta tagttcatga aataaactcc ggtgaatttg
     1261 agtatttaag acgttttccc cagtccagca ctggggcaaa aatggtgaca acgagagtca
     1321 taaaaacatt cggggagctt tgtgatatct ggacaaaaat taaagagaca gagttaacaa
     1381 caaacacaat gaagaaaacg aaatcacaat taaaaacact cagaataata atttgtgaaa
     1441 gtaccccgat atcatatatt cgttatagcg atatcttaaa ctaccggaat gaactgctgc
     1501 atggagaaac gctttacctg gataatccaa gatccaacaa aaaaggaaga accgtgcgca
     1561 cagttgataa ctatatcgcc ctgctctgtt cgctgttacg ttttgcgtat cagtcgggat
     1621 ttatatcaac caaaccattt gaaggagtaa aaaaattaca gcgaaacaga ataaagcctg
     1681 acccgttatc taaaacagaa ttcaatgcat taatggaaag tgaaaaagga cagagccaaa
     1741 acttgtggaa atttgccgtt tactccgggc ttcgtcacgg ggaactggca gctctggcgt
     1801 gggaggatgt ggatttcgag aagggaattg tgaatgtcag aagaaacctg acgatacttg
     1861 atatgttcgg tcccccaaaa acaaatgccg ggatccggac ggtaacatta ctacagccag
     1921 ctcttgaagc actgaaggag caatacaaac tgaccgggca tcatcgcaaa agcgaaatca
     1981 ctttttatca tcgggagtac ggcagaaccg aaaaacaaaa actgcatttt gttttcatgc
     2041 ccaggatgtg taacggaaaa cagaaacctt attactcggt aagcagtttg ggtgcgagat
     2101 ggaatgcagc agtaaaacgt gctggtattc gccgccgtaa tccgtaccat acgcggcata
     2161 cttttgcctg ctggctgttg acggcaggag cgaacccggc gtttatagcc agccagatgg
     2221 ggcatgaaac tgcgcagatg gtgtatgaaa tttacggtat gtggattgat gacatgaacg
     2281 acgaacaggt agccatgttg aatgcgcggt tatcgtagtt gcaaagtttg cccccaattt
     2341 gccccattta gtaccagaga actgaaataa tgaaagaaaa tcaacaaatt acaaagaaag
     2401 aacaatacaa cctgaacaaa ttacaaaaac gtctgcgtcg taacgtgggc gaagccattg
     2461 ctgacttcaa tatgattgaa gaaggcgatc gcatcatggt ttgcctctcc gggggtaaag
     2521 acagctatac catgctggag attctgcgca atttgcagca aagcgcgcca atcaattttt
     2581 cgctggtagc tgttaacctc gatcaaaagc aaccgggctt cccggaacac gttctgcccg
     2641 agtatcttga aaagctgggc gttgagtaca agattgtcga agagaacact tacggtatcg
     2701 tgaaagagaa gattccagag ggcaaaacca cttgctcact gtgttctcgc cttcgtcgcg
     2761 gtatccttta tcgtaccgca acggaactgg gggcgacgaa gatcgcgttg ggccaccatc
     2821 gtgacgatat cctgcaaacg ttgttcttaa atatgttcta cggcggcaag atgaaaggta
     2881 tgcctccgaa actgatgagc gatgatggca aacatatcgt tattcgtccg ctggcctact
     2941 gccgcgagaa agatattcag cgatttgccg atgcaaaagc gttcccgatt attccgtgca
     3001 acctgtgcgg ttcacagcct aacctgcaac gtcaggtgat tgctgacatg ttgcgtgact
     3061 gggataaacg ttatccaggg cgtatcgaga cgatgttcag cgcgatgcag aatgtggtgc
     3121 cgtcgcatct gtgcgatacc aacctgttcg atttcaaagg cattacccac ggttctgaag
     3181 tggttaacgg gggtgatctg gcgtttgatc gcgaagagat cccactacaa ccggcgggct
     3241 ggcagccaga agaagatgaa aatcagttgg atgagttacg gctgaatgtg gttgaagtga
     3301 aataaccagg atcgcgcccg atgcgcaagc gtatcgggct actcttatgg aggccggata
     3361 agacgcggcc agcgtcgcat ccggcaatcc cgaataagat gtttactctt gcacccggca
     3421 attcaacatt tcattatttt aataaccgca cccggcacgt ttttccttta atcttcccgc
     3481 cctgtaactg tttccatgct ttatgagcaa cagcctgacg gaccgcgaca tagacatgcg
     3541 ccggatgcac ggcgattttg ccaatatctg cgccatcaag cccgatatct cctgtcagtg
     3601 cacctaaaac atcacccggg cgcattttgg cttttttccc gccatcgata cacaacgttg
     3661 ccatttctgc ttccagcggc acaatggaac tattagctgg cggcgtttgc cagttaagtt
     3721 ttatctgcaa catgtcagaa atgatattgg cccgctgtgc ttcttccgga gcacagaaac
     3781 tgatcgccag accgctattt cctgcacgag ctgtacgacc gatgcgatgt acatgaactt
     3841 cagggtccca cgccagctca aagttcacca ccagctcaag cgatttaata tccagaccgc
     3901 gcgcagcaac atcagtcgca accagtacac gggcgctacc gttagcaaaa cgtaccaggg
     3961 tctgatcgcg atcgcgttgc tccagatcgc cgtgtaacga caatgcactt tgccctactt
     4021 cattcagcgc gtcgcagaca gcctggcaat cttttttggt attgcaaaac accacacaag
     4081 aggatggctg atgcaagctt aataaccgtt gcaacagagg aattttgcct ttactggatg
     4141 tctcataaaa ttgttgttca atgggtggca aagcatctgt tgagtcaatt tcaatcgcca
     4201 aaggatcgcg ttgcactcgt ccgctgattg cggcgatggc ttccggccag gttgccgaaa
     4261 acagaagcgt ctgtcgagat gcaggcgcaa aacggatgac atcatcaatg gcatcgctaa
     4321 atcccatatc cagcatgcgg tcggcctcat ccatcaccaa agtattcagc gcatccagtg
     4381 ataccgtgcc tttttgcaag tgatccagca aacgacccgg cgttgccacg ataatatgcg
     4441 gcgcatgttg caacgaatca cgctgcatac cgaacggttg accaccgcac aacgtcaaaa
     4501 ttttggtatt tggcagaaaa cgcgccagcc gacgcaattc accagccacc tgatccgcca
     4561 gttcacgcgt aggacacagc actaaagcct gggtctgaaa tagcgacgca tcaatttgct
     4621 gtaacaagcc gaggccaaaa gccgccgttt tgccgctgcc ggttttcgcc tgcacgcgaa
     4681 catcttttcc ggcaaggatc gccggaagcg cggcggcctg caccggcgtc atggttaaat
     4741 aacccaactc attaaggttc gtgagttggg cgggaggcaa aacattcagg gtagaaaaag
     4801 cggtcacaat ctattctcgt ggtcatcgac gcaaagttag caggcgcgta tcctcgcaga
     4861 tctacgctca cgatgcgaca atttaatcgg ttcttcatcg ggtggtgggt caggcatggg
     4921 ttgcgggcga gggatcggat cgggcactgg aacaggatcg ccaggaatcg gttcagggac
     4981 aggaatttgc aaataaataa gtgtcgtcat atttccctcc ggtcattggg tggactctta
     5041 aagggtagac gctgataaat aacaggcaaa aaaaagccga ctcatcaaag tcggcgtcgt
     5101 acgaatcaat tgtgctatgc agtaattcaa aaaaggaagt aagacaatat ggagcgcaac
     5161 gcccatcgct tgacgttgca ttcacctgca agagagatat tgccctgaat gggtagagag
     5221 tttattgact tcgctcaaac tttgcggcgt ttttgtatac agacagccgg aaaaattgct
     5281 tttgttacaa ccatttacta cgatgcaacc ataaagcaac accaccaata agaacaacta
     5341 acagaataca aaaaattgaa aatccgaatt gccacccgcc gccagggatc ccaccaaggt
     5401 tgacgccaaa taaccctgtc agaaaggtac tgggtaaaaa gaccattgcc atcaacgaca
     5461 ttgtataggt acgacgagct aaattttcct gcatcacctg agcgatttca tccgccatca
     5521 cgccagtccg tgctatacag gcgtcgattt cgtcaaggcc gcgcccaagg cgatcggcaa
     5581 tatcctgcat ccgacggcgt tggtcatcgc tcatccacgg caaacgttca ctggcaagac
     5641 gagcataaac atcacgttgc ggtgccatat agcgacgcat cacgattaat tgtttgcgca
     5701 gcagagccag gaatccacgc ggtggaattt gctgatcaag gagattatct tcaaggtcga
     5761 taattttatc gtgcagctgc tcgataaatt cgctggaatg atcggtcaac gcatcgcaca
     5821 catccaccag ccaccctccg caatcggtcg gacccgtgcc ctcttccaga tcgctcacca
     5881 catcgtccag cgccagcact ttgcgttgtc gggtcgaaac aattaaccgc ccgtccatat
     5941 atacacgcat ggcgaccagt tgatcggggc gttcatcggt gctgccgttt atacagcgca
     6001 atgtaatcag cgtgccttca ccgagacggc tgactcgggg ccgtgtgctc tcgcccgcca
     6061 gcgcatcacg tacgttattg ggaagcagcg gtgttgtcgc cagccattgg gcgctatcat
     6121 ggtgtacata attaaggtgg agccagcagg gatgcgcttc atcaatcaca tctgtatttt
     6181 ccagcggttt aacgccgcct ctaccatcca gcatccaggc aaatactgca tccgggacat
     6241 taacgtccga tcccttaatc gcttccacag tgcctccatc atcaacgcat tattttgtag
     6301 tctagccttc tggccctgtt acgcaacatc tcatcacccc attaccctga aatgattaat
     6361 aaaattctgt ctaaattgaa tacaaaaagc aaaatgcttt tccgtataca aaccgtgtga
     6421 agtgttaaat agcgtctatc attatcagaa ttatctgatc atatgacgtg gcttttttgc
     6481 gatcggatag caacaaaaat tgataaaaat aacgggatct caatgattac gcacaacttc
     6541 aataccctgg acttactcac cagtcctgtc tggatcgttt cgccctttga ggaacagtta
     6601 atttatgcca atagcgcggc gcgactgttg atgcaagacc tcacgtttag tcagctacga
     6661 accggtccct attccgtctc ctcacaaaaa gaactgccga aatacctctc cgatctgcaa
     6721 aaccaacacg atattatcga aatcctcact gttcagcgta aagaagagga aacagcatta
     6781 agctgtcggc ttgttttgcg agagctgaca gaaacagaac cggtgattat tttcgaaggt
     6841 atcgaagcgc cggcaacgct gggtttaaaa gccagtcgct cggcaaatta tcagcgcaaa
     6901 aaacaaggtt tttatgcgcg cttttttctg actaactctg caccaatgtt gttgattgac
     6961 ccctcacgag atggccaaat cgtcgatgct aacctcgccg cgctcaattt ctatggttat
     7021 aaccatgaaa cgatgtgcca gaaacatacc tgggaaataa atatgctcgg gcgtcgcgtc
     7081 atgcctatca tgcatgaaat ttcgcattta cccggcggtc ataaaccttt gaattttgtt
     7141 cataaactgg cggatggttc gactcgtcat gtgcagacct atgccgggcc gattgaaatt
     7201 tatggcgaca agctcatgtt atgtattgtg catgatatta ctgagcaaaa acggctggag
     7261 gagcagttgg aacatgctgc ccaccatgac gcgatgaccg gattactgaa tcggcgacag
     7321 ttttatcaca ttacggaacc aggccaaatg cagcacctcg ccattgccca ggattacagc
     7381 ttgttgctca tcgacaccga tcgttttaaa cacattaacg atctctatgg gcattctaaa
     7441 ggtgatgagg tgttatgcgc cctcgcccgc accctcgaaa gttgcgctcg caaaggcgat
     7501 ttggtgtttc gttggggagg cgaagagttt gtcttattgc taccaagaac cccactggat
     7561 accgcgcttt cgctggctga aactatccgc gtaagcgtgg caaaagtgag tatttcgggc
     7621 ttaccacgct ttaccgtcag cattggtgtg gcgcatcacg aaggaaatga aagcatcgat
     7681 gaactgttta aacgcgtaga tgatgctttg tatcgggcga aaaatgatgg acgcaaccgc
     7741 gtgctggcgg cataagccgc ggatgcgtct cgagatcaac gactgcgctt agcgtggcgc
     7801 tcccagtttt cttgcttcgc ctgcgccgtt ttacgtagtg cgacgtaaca cgccccgctg
     7861 ccgccatgat gcggtagcgc ggtgcaatat gcctgaacat catcaaattc ggtcagccag
     7921 cgcgccacat agctgcggac aatattggca tgcgatttat cttcccgccc tttaccatga
     7981 ataatcagca cattacgcaa accatccgcc agggcttgtt gaatgaaact gaacaccatt
     8041 ttgcggcact cttccaccgg ctggcgcaaa agattcaggc tcgcctgttg cggatattta
     8101 ccactgcgca gcttatccag caccccatgt tgcaaccctt cccgccgaaa ctccagcggc
     8161 tgacttagtg ggatgatgtc gagaaatccg gtggtgagga aattatcaag ttgcagcgtg
     8221 tcgatacgct gcggcgcacg ttggttacgc gttggatgcc agtggacatc ggtagcacgt
     8281 ttcagcggct ggacatcttc catggcgtca agaaacagcg atttgtcgtc aaggttcatg
     8341 ttacatcctc ccgcaattaa gagcgcgata tgataaccag accgggtcgg tccaacaacg
     8401 tattacccaa aattccagta ataagttcca aatattgccg atattttaag caaaatgctt
     8461 atgcatgatt attcattcac gatattaata atgtaactta tattttcgtg aaatctgtca
     8521 ctgaagaaaa ttggcaacta aaggttaaaa ccgttataac acagtcaccg gcgcagagga
     8581 gacaatgccg gacttaagac gcggatgcac tgctgtgtgt actatagagt ctggcggatg
     8641 tcgacagact ctattttttt atgcagtttt aactttgcag atagccgcat tctcgccgaa
     8701 attgcgtgat taatgacgcg gtcagtggtg tctggcgact atcacgccgc tgaatcaaat
     8761 aataggccgc tttcggtaaa atttcgctaa ccggcaacat caccagcccc tgtccgtgca
     8821 agggatcgca gcccatttct tcaggcagta tgctgagaaa atcgcttttt gccaccagac
     8881 tgatacaggc tgagaacgtc tcgcagacta caccgacctg tggcgtttgc gcctgatcgt
     8941 caagcaattc actcaactgt ttgtagtagc tgccgtgtgg cgtcggcatt gtccagctgt
     9001 aatccagtaa ctgtttgatc gaacgggcac caatggcggg gtgtcccggg cggcaaaaga
     9061 tcgcgaattg cttttccaat aatttctcaa aagtaaattc gtggtcgtac ggtccctgat
     9121 aataggtatt gatggtgaaa accagttctc cctgacgcaa ttcattaatc atcgacacca
     9181 gttgcccttc cataatgcgt acttttacct gcggatgctg ctgatgaaaa cgagatatga
     9241 cagctggcat cagactgcgg gaaatactgg cccccatgcc gatattaatc tgccctgcca
     9301 gttgcccttg tcgttggcga atatcctctt gggctgcgcg gagctcttca agaataaggc
     9361 tggcgtgctg ataaaaactt tcaccggcat cagttaacgt cacgccttta ctacggcgaa
     9421 aaaagagttg cgccgctaac ccttcttcca gctcctgaat agatttactc agtgccggtt
     9481 gcgacatatt caacattcgg ctcgctccgc gaatgctgcc ctgacgagcc acttcaacaa
     9541 aagcccgaat ttgatgaatt tttacctgaa aagccatgac gccaccgata accgttattt
     9601 atcagaccaa agaaactggc atctacttta atgcagatga ttgtgtcagg gtaatttatg
     9661 aacggttaaa actgtgaaaa atcagttagt gataagtaaa aactatcgct acgtgaaccg
     9721 ggtcacactt tttactgatg acgggaaagg ttatggagtc tttgaatcaa tttgttaatt
     9781 cacttgcccc aaaattatcg cactggcgac gtgattttca tcactatgca gagtctggct
     9841 gggtggaatt ccgcactgcc acccttgttg cggaagaatt gcagcagctc ggctattcac
     9901 tggcactggg ccgcgaagta gttaatgaaa gtagccggat gggactacct gatgaactca
     9961 ctctacaacg cgaattcgag cgcgctcgtc aacagggtgc gctagcacaa tggattgcgg
    10021 cttttgaagg tggtttcact ggtattgtcg ccaccctgga taccggtcgc cccggtccag
    10081 ttatggcttt ccgtgtcgat atggacgcgc tggatctcag tgaagagcag gatgtcagcc
    10141 atcgccccta ccgcgacggt tttgcgtcat gtaacgccgg aatgatgcat gcctgtggtc
    10201 atgatgggca taccgccatt gggcttgggc tggcgcatac ccttaaacag ttcgagtccg
    10261 gactacatgg cgtcatcaaa ctgatttttc agcctgcaga ggaaggtacg cgtggcgcgc
    10321 gggcgatggt cgatgcaggt gtcgtagatg atgttgatta ttttactgcc gtgcacattg
    10381 gcactggcgt acctgcgggc accgtggtgt gcggcagtga taattttatg gcaaccacca
    10441 aatttgacgc gcacttcacc gggaccgccg ctcacgcagg cgcaaaacca gaagacggtc
    10501 acaatgcctt gttggcggca gcacaagcaa ctcttgcact gcatgcaatc gccccgcaca
    10561 gcgaaggagc ttccagagta aacgtgggcg ttatgcaggc aggaagcggc cgtaacgttg
    10621 ttcctgcctc ggcgttgctg aaagtggaaa cacgcggggc cagcgacgtc attaatcaat
    10681 atgtttttga ccgtgcacaa caagcgattc agggcgcagc aaccatgtat ggtgtcggcg
    10741 ttgaaactcg tctgatgggt gcagctaccg ccagttctcc ttcgccgcaa tgggtcgcat
    10801 ggttgcaaag tcaggcggct caggtcgcgg gggtcaatca ggccattgaa cgtgttgaag
    10861 cgcctgcggg ttccgaagat gccacattaa tgatggcccg cgtgcagcaa catcaagggc
    10921 aagcctccta catggtgttt ggcacacaac tggcggcagg tcaccacaac gaaaaattcg
    10981 attttgacga gcaggttctc gctattgccg tcgaaacgct ggcgcgcacc tcgctcaatt
    11041 ttccctggac gcgaggtatc tgatgcagga aatctatcgt tttatcgacg atgcgattga
    11101 agccgatcgc caacgttata ccgatattgc cgatcaaatc tgggatcatc cagaaacacg
    11161 ttttgaagag ttctggtcag cggagcatct ggcttcggcg ctggaatctg caggcttcac
    11221 cgttacccgc aacgtaggca atatcccaaa tgcctttatt gcttcgtttg gtcaaggcaa
    11281 accggttatc gccctgctgg gagaatatga cgccctggca ggtttaagtc agcaagctgg
    11341 ttgcgcgcaa cctacatccg cgacgcccgg tgaaaatggt cacggttgcg gacacaattt
    11401 gctgggaacc gccgcctttg ccgctgcaat agccgtcaag aaatggctgg aacaatatgg
    11461 gcaaggcggc acagtacgct tttatggttg tcctggcgaa gaaggcggct cgggtaaaac
    11521 gttcatggtc cgcgaggggg tatttgatga tgtggatgcg gcactcacct ggcacccgga
    11581 agcctttgcc ggtatgttca atacccgcac gctggcaaac attcaggcat catggcgctt
    11641 taaagggatc gcagcacatg ccgcgaattc ccctcatttg ggacgcagcg cccttgatgc
    11701 cgtaacgttg atgaccactg gcaccaactt cctcaacgaa catattattg aaaaagcgcg
    11761 cgtacactat gccatcacag atagcggcgg gatcacgccc aacgtggtcc aggcgcaggc
    11821 agaagtactt tatcttatcc gcgcccccga aatgaccgac gtgcagcata tttatgatcg
    11881 ggtcgccaaa atcgccgaag gtgcggcatt gatgaccgaa accacggttg aatgccgctt
    11941 cgacaaagcc tgttccagtt atctcccgaa tcgcacctta gaaaatgcca tgtaccaggc
    12001 cctatcccat tttggtaccc cagaatggaa ctccgaagaa ctggcttttg cgaaacaaat
    12061 tcaggctacg ctcaccccca acgatcggca aaacagtctg aataatatcg ccgcaaccgg
    12121 tggcgaaaac ggcaaggttt ttgcactacg tcatcgtgaa acggtactgg cgaatgaagt
    12181 cgctccatat gccgccaccg ataacgtgct tgcggcatcg actgatgtcg gcgacgtcag
    12241 ttggaaactg cctgttgccc agagtttcag cccctgtttt gccgtcggta caccgctaca
    12301 tacgtggcaa ctggttagcc aggggcgaac atctattgct cataaaggaa tgctgctggc
    12361 ggcgaaaact atggcagcaa ccacagtcaa tctcttcatt gattcagggc tattgcaaga
    12421 atgcctacaa gagcatcagc aagtaacgga cacgcaaccg tatcactgcc ctatcccgaa
    12481 aaacgtgaca ccgtcacctt taaaataaca acaacaacgt aaacacaaca accgaggaat
    12541 gcccatgagt atgtcatcca taccgtcgtc ctcccaatcc gggaagctct atggctgggt
    12601 cgaaagaatt ggtaacaagg ttccccatcc ttttttgctc tttatctatt tgattatcgt
    12661 actcatggtg acgacggcaa ttttgtcggc ctttggcgtc agtgcgaaaa acccgaccga
    12721 tggtacgccg gtggtggtga aaaacctgct cagtgtggaa ggattacact ggtttttacc
    12781 caatgtgatt aaaaacttta gcggttttgc tccacttggt gcgatcctgg cgctggtttt
    12841 aggtgccggt ctggcggagc gcgtcggctt actgccagcg ctaatggtta aaatggcatc
    12901 gcatgttaat gcccgctacg ccagttatat ggtgctgttt attgcttttt tcagccacat
    12961 ttcttccgat gcggcgttag tgatcatgcc accgatgggt gcgctgattt ttctggcggt
    13021 gggcaggcat ccagttgcag gtttactggc cgccattgca ggcgtaggtt gcggcttcac
    13081 ggctaattta ctgattgtca caaccgacgt gttgctgtcg gggatcagca cggaagcggc
    13141 ggctgcgttc aatccgcaaa tgcacgtcag tgtaattgat aactggtatt ttatggccag
    13201 ctccgtagtc gtactgacga ttgttggcgg cctgataacc gacaaaatca tcgaaccacg
    13261 gttaggtcaa tggcagggaa acagcgatga gaaactgcag acattgaccg aaagtcagcg
    13321 ttttggttta cgcatagcag gtgtcgtatc gctacttttt attgctgcga tcgcgctgat
    13381 ggtgatcccg gaaaacggga tattgcgcga tccgattaat cacaccgtga tgccatcacc
    13441 ctttattaaa ggtatcgtgc cactgatcat tctttttttc tttgtggttt cgctggctta
    13501 tggcatcgct acccgcacaa ttcgacgtca ggcggattta ccgcatttaa tgattgaacc
    13561 gatgaaagag atggcgggat ttatcgtgat ggtttttccc ctcgcccaat ttgtcgccat
    13621 gtttaactgg agcaacatgg ggaaattcat cgccgtgggg ctgaccgata tactggaaag
    13681 ttcagggctt agcggcatcc cggcgtttgt cggtctggcg ttgctttcct ctttcttatg
    13741 catgtttatt gccagcggtt ccgcaatctg gtcgattctg gcccccattt tcgtaccaat
    13801 gtttatgcta cttggctttc acccggcatt tgcgcaaatc ctctttcgta ttgccgactc
    13861 atccgtattg cctttagcgc cggtatctcc ttttgttcca ctgtttcttg gattcctgca
    13921 acgctacaaa ccagacgcga aactgggtac ttactattcg ttagtcttgc cctatccgct
    13981 tatctttttg gtggtatggc tgctgatgtt gctggcgtgg tatcttgttg gtctgccgat
    14041 aggtccgggg atttacccac gtttgtctta agagagaacg gatgctgaga ttacttgaag
    14101 aaaaaattgc cacgccactg ggtccactgt gggtgatttg cgatgagcaa tttcgcctgc
    14161 gggcggttga atgggaagag tacagcgaac gcatggtgca gctgctgaac atccattatc
    14221 gcaaagaagg ctatgagcgc atttctgcca ccaacccagg cggtttaagc gacaagcttc
    14281 gtgattattt tgccggtaat cttagcatta ttgatacgct tcccactgcc acagggggga
    14341 cgccatttca gcgcgaagtc tggaaaacac tacgcactat cccctgcggg caggtaatgc
    14401 attacggcca actggctgaa caattgggcc gccctggcgc ggcgcgtgcc gttggtgcgg
    14461 caaacggatc gaatcccatc agcattgtag taccttgcca tcgggttatt ggccgaaacg
    14521 gcaccatgac cggatatgca ggcggagttc agcgaaaaga gtggttattg cgccacgaag
    14581 gttatctttt gctgtaaaca ttaaacaatt tgtgccagct tgttcacact tttatgtaaa
    14641 gttaccctta acaacttaag gtttttcaaa tagatagaca tatatttaca tctaatatcg
    14701 gaattctctg ctgttaaggt ttgcttagac ttacttgctc cctaaaaaga tgttaaaatt
    14761 gacaaatatc aattacggct tgagcagacc tatgatcccg gaaaagcgaa ttatacggcg
    14821 cattcagtct ggcggttgtg ctatccattg ccaggattgc agcatcagcc agctttgcat
    14881 cccgttcaca ctcaacgaac atgagcttga tcagcttgat aatatcattg agcggaagaa
    14941 gcctattcag aaaggccaga cgctgtttaa ggctggtgat gaacttaaat cgctttatgc
    15001 catccgctcc ggtacgatta aaagttatac catcactgag caaggcgacg agcaaatcac
    15061 tggttttcat ttagcaggtg acctggtggg atttgatgct atcggcagcg gccatcaccc
    15121 gagcttcgct caggcgctgg aaacctcgat ggtatgtgaa atcccgttcg aaacgctgga
    15181 cgatttgtcc ggtaaaatgc cgaatctgcg tcagcagatg atgcgtctga tgagcggtga
    15241 aatcaaaggc gatcaggaca tgatcctgct gttgtcgaag aaaaatgccg aggaacgtct
    15301 ggctgcattc atctacaacc tgtcccgtcg ttttgcccaa cgcggcttct cccctcgtga
    15361 attccgcctg acgatgactc gtggcgatat cggtaactat ctgggcctga cggtagaaac
    15421 catcagccgt ctgctgggtc gcttccagaa aagcggtatg ctggcagtca aaggtaaata
    15481 catcaccatc gaaaataacg atgcgctggc ccagcttgct ggtcatacgc gtaacgttgc
    15541 ctgatttttc cgcataactc actatccttc tgtcatatca ttaaattttt ctgatttatt
    15601 gatctggcag aaggttcatc actgtttcat tcaccagata tgggttaatc ttttaattac
    15661 aaactgcgtt gacagttgtt gtaaggagac cctgtatggc tatgtatcag aacatgctcg
    15721 ttgttatcga tcctaaccag gacgaccaac cagcattgcg gcgagctgtt tatttacatc
    15781 aacggattgg tggcaaaatt aaagcctttt tgccgatcta tgacttctca tacgaaatga
    15841 ccaccctgct ctccccggac gaacgtaccg ctatgcgtca gggcgtcatc agccagcgta
    15901 cagcctggat ccacgagcag gcaaaatatt atctcaatgc tggcgttccc attgaaatta
    15961 aagtggtctg gcataaccgt cctttcgaag ccatcattca ggaagtgatc agcggcggac
    16021 acgatttggt gctaaaaatg gcgcaccaac atgaccgtct ggaagcggtg atttttacgc
    16081 caacggactg gcatctgtta cgcaaatgcc caagcccggt gtggatggtg aaagaccagc
    16141 cgtggccgga aggaggcaag gcgctggtgg cggtgaatct cgccagtgaa gagccgtatc
    16201 ataatgcgct caatgaaaaa ctggtcaaag agacgatcga actggcagaa caagtcaacc
    16261 ataccgaagt tcatctggtt ggcgcttatc cggtaacgcc aattaatatc gcgattgaac
    16321 tgccggaatt tgacccgagc gtttataacg atgccattcg tgggcaacat ttgctggcaa
    16381 tgaaagctct gcggcagaaa ttcggcatta atgaaaacat gacgcacgta gaaaaaggtc
    16441 tgcctgaaga ggtgattcct gatttggcgg agcatttaca ggcgggaatt gtggttctgg
    16501 gcacggtcgg acgcaccggt atttcagcag cattcctcgg caacacggcg gaacaggtga
    16561 ttgatcatct tcgctgcgac ctgctggtca ttaaacctga ccagtatcag acacccgttg
    16621 aactggatga cgaagaagac gattaacgac tcttcaataa ctgacaaggc ccgagttatc
    16681 gggcctattt tttacttact gctcttcgcc gccaaggaaa taaattccca acgggattgc
    16741 cagcaacaca gtaaagacca ggctataaac aaaaatcatc gatgactgaa tcacatacat
    16801 tgatgtcgtc cactcagaca gcggaatatt gtactgttct ataacgccgc cgatggtcgc
    16861 gcgagtcaca accgacgccg caataatgaa aaccgccaac aaaccaaaga gaaatttctt
    16921 ccctttcgct gacttcagtt tcgccataat catgatgtaa tccttgtgca gacaaatttc
    16981 ttgttaccta aacgtaacaa taccatgaca tttcaccgtt gtctgctctg aaaacctcac
    17041 ctcttagctg gatttacaat caacgcattg attagttagc taatgttgct acttccttcc
    17101 ctgactattg atgatctgaa tgaattgtcg cggtaaaata gccccgccag gggtttccgg
    17161 catgacgaca attatctgga ggagcctgat aaaacaatga tcgctgaact gtttacaaat
    17221 aatgcgctta atctggtcat tattttcggt agctgcgcag cattgattct gatgagcttt
    17281 tggtttcgcc gtggaaatcg taaaagaaaa ggatttttat tccatgcggt gcaattttta
    17341 atctacacca taattatcag tgctgttggt agcattatta attatgttat agaaaactat
    17401 aaactcaaat ttatcacccc aggcgttatc gattttatct gtacgtccct gattgcggtt
    17461 attctgacga ttaagctatt tctgctgatt aatcagtttg aaaaacagca gattaaaaaa
    17521 ggtcgcgata tcaccagtgc gcggattatg tcgcgtatca tcaaaatcac cattattgtg
    17581 gtgcttgttc tgctttatgg cgaacatttc ggcatgagcc tttctggctt gctgaccttt
    17641 ggtggtattg gtggtctggc tgtcggtatg gccggtaaag atattctgag taacttcttt
    17701 tccgggatta tgctctattt cgaccgtcct ttcagtattg gcgactggat ccgttcaccg
    17761 gacagaaata tcgaaggtac agtagcggaa attggctggc gaattaccaa aattacgacc
    17821 tttgataatc gtccattgta cgtaccgaac tcgctgtttt cgtcgatcag cgtagaaaac
    17881 ccaggacgaa tgaccaaccg ccgcattacc acgaccattg gtttacgtta tgaggatgcg
    17941 gcaaaagtgg gcgttattgt cgaagctgta cgtgagatgc tgaaaaatca cccggccatc
    18001 gaccagcgac aaaccttact ggtttatttc aaccagtttg ctgactcttc attgaatatt
    18061 atggtttatt gctttaccaa aaccacggta tgggctgaat ggcttgctgc acagcaagac
    18121 gtttatttga agattatcga tattgtacag tcacacggcg cggactttgc cttccccagc
    18181 cagacgctgt atatggataa cattacaccg ccggaacagg gtcgctaaat cagacatcac
    18241 tggcgttatg cacaagggta acgccagttc ccatcacatc aatgcttcac aatatacata
    18301 gtccgactgt acgccacatc ttcaggatta ttaatgggat aacctttcag ccacggcttg
    18361 attaatcgtc cattggtata ttgataaatt ggtgcaatcg gtgcttgctc catgaggatt
    18421 ttttctgccg cgttgtaatc ggcattacgc gctttaacgg tattttccgt cgatgcctgg
    18481 gccagaactt tgtcatatgc cggattgtta aagcgtgaaa tatttcctga atgcgttgac
    18541 gttaataatg tcaggaaagt ggagggttca ttataatccc ccacccacga ggcgcggata
    18601 acatcaaaat tgccggtgtt acggctatcg atataggttt tccattcctg attttgcaat
    18661 ttaacatcta cgccaaggtt ctttttccac atcgatgcta cagcaatcgc aattttttga
    18721 tggttttctg aagtgttata caaaagcgtc agcttcagcg gtttttgcgg accataacca
    18781 gctgcgctca acaaagtttt tgcctgcgca ttcagttctt cctgactcat ttgttcaaac
    18841 ggcgaaggtt ccggcgtaaa tcccgcggta acatctggtg taaaatgcca cgctggcttt
    18901 tcgcccgtcc ctaatacttt ttcggtcatc aggcggcgat ctatcgtcat acttaatgcc
    18961 agacgaacgc gctgatctgc cgtcggccct ttttgcgtgt taaacgcata ataataggtc
    19021 ccgagctgcg gcggcgtata aacctgcccc ggaatatcct tcaacagctt ctgatacata
    19081 tttttcggga aggattcggt gatatcaata tcccccgcga ggtaacgctt agtggctgcg
    19141 gattcctgat taattggcag gaaggtcact ttttgcagta ccgttttggc gttatcccaa
    19201 taatgggtat tcggtaccac gaccagtttt tcattgacta cgcgctcttt aagaacataa
    19261 gcgccattgc cgatcagatt tccgggtttc gtccactctt taccgctttc tacgttggct
    19321 ttttgcaccg ggaagaaggc aaagttagcg gttaaattca caaaccacgg caacggttta
    19381 tcaagctgaa ttttcaaagt atgggcatca actgcggtga cgccaagctg gtcaggcgta
    19441 gctttaccat caataatcgc ctgtgcgttg ttgattcccg ccagcgcggc aaaccatgca
    19501 aatggcgaca atgtttttgg gtccaccaga cgttgccagc tgtagacaaa atcttgcgcc
    19561 gttaccggtg tgccatccgc ccattttgcg ttatcgcgca gggtaaaagt ccagatacgg
    19621 ttgtcattac ttttccactg agtcgcaacg ccggggacaa tctccccttt ttcgttctga
    19681 ttcaccagac cttcaaacag atcgcgaatg acctgaatct ctggcaggcc cacggcttta
    19741 gcgggatcca gcgacgcagg ctcatcttta atgtggcgca ccagctcctg cttctctgcc
    19801 agtactgtgc cgctcggaac ttctgcagca tacgaaagag aaatgctgct gaccaacagc
    19861 gcacaacacg tgactgaaac agagtgcttc ataagtttcc ctctaaccga cattaaaatt
    19921 gatgcgtaat tatttgtttc gccttaaaga aatgcaaaca acttccatca aaaaagtgac
    19981 aaaggaagca tcaaatgttt taagtatcaa tatatcaaat cagatttagt gtaaataata
    20041 gtctccaaaa tgcggtaaag cacatagaac tccgaacact acgttcgggc ttcgcacgtt
    20101 caaacccgtt ttcggtatca taatcagcat ctgtcgccat tgtacgaaga ataaaaaatg
    20161 accaaaacgt tgaaaggaac tgagaatacg ttaagagaag aattaaatta atgtatcagc
    20221 aagtagtgct aaacagcacc tttatacttc agcctatcta taaataatga gaatgccgag
    20281 ccagcatgtc gacggtgtgg atagtacagg tgataaccgg gtaaatctgg tgtgaattta
    20341 tccaaaacac gtatcagttt tttttcttta atagcccgct caacctgatc ataaggtaga
    20401 tacgccaatc catgcccatc aattgcagca tcaatgatca ggtctatcgt attcagtaaa
    20461 agctgacctt ccatgcgaac acgaacttca cgtccaccgc gtattaatct ccagcgattt
    20521 gctgtacccg atgtgggaag atacaaatta attgcctgat gatctattaa ttgtgacact
    20581 gacgttggaa cacttcggcg agaaaaataa tccggtgagc caacaatagc cattggtata
    20641 tctggcccga ttcgaatggc gatcatatct ttatccatct ccccacccag acggacgcct
    20701 gcatcaaaac gttcagaaac gacatcggtc aaaccataat caatggtgag ctgaatatca
    20761 atttcaggat gcgatttcag gaatgtgcgc attgctggta acaatatcgt ttttgctgca
    20821 tgttctacag tagtaatacg tattgtcccg gatgggcggt tctgcagatc gctcagggat
    20881 gccagggctg aatctatgtc atgcaacata gggccaagaa cagacaaaag atgctcgccc
    20941 gcttcagtgg gtacaacact gcgcgtggtt cgggtcagaa gccgcaatcc caatcgttct
    21001 tctatacggc gcactatctg gcttaaagct gactgcgcca tgctcaggcg ggctgctgct
    21061 cgagtgaagc tacgctcctc tgcaacgacg acaaacgcca tcagatcagc gatctcttca
    21121 cgcttcattc agtatctcca tacaatctat taatcattta aatgataggt ctaatcatga
    21181 ttttgggtct aatcaactta atgatcaata tctatagtga tatcaccaac cgttaaaacg
    21241 aaccaacaac tccatgagga acggaatgat gaataataaa gtcagcttca ctaacagcaa
    21301 taatccaacc atctctttgt ctgcagtgat ctattttcct ccaaaatttg atgaaacccg
    21361 tcaatatccg gcaattgtgg tatctcaccc aggtgggggc gttaaagaac aaacggccgg
    21421 gacctatgcc gaaaaactgg cggaaaaggg atttgtcact attgcttatg acgcatctta
    21481 tcagggtgaa agtggcggcg agccgcgtca gttagaaaac ccatacattc gtactgaaga
    21541 cgtcagtgcc gtgattgatt acctgacaac gctttcttat gtcgacaata cccgaattgg
    21601 tgcgatggga atttgtgccg gtgcaggata tacagccaat gctgcaattc aggatcgccg
    21661 catcaaggcc atcggtaccg ttagtgcagt caatattggt tcgatgttcc gtaatggttg
    21721 ggaaaacaat gtgaaatcta ttgatgccct gccatacgtt gaagcgggtt caaacgccag
    21781 aaccagcgat attagcagcg gagaatacgc cattatgcca ctggcaccaa tgaaggagtc
    21841 cgatgcgccg aacgaagaac tgcgtcaggc ctgggagtat taccacaccc ctcgcgcgca
    21901 gtatccaaca gcaccgggtt acgctacttt gcgcagcctt aaccagatta ttacctatga
    21961 tgcttaccat atggcggaag tgtacctgac tcagccgacg cagattgtgg cgggcagcca
    22021 ggcagggagc aaatggatga gtgacgacct gtacgatcga gcatcaagcc aggataaacg
    22081 ctatcacatt gttgaaggcg caaaccatat ggatctttat gacggcaaag cctatgtggc
    22141 tgaagcaatt tcagtattag ctccgttctt tgaggaaact ctgtaatgaa acatattcaa
    22201 atccgcaata gtgatatggc ctggcatatt gctgccaata atctgggata attttcatga
    22261 aaaaggtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
    22321 cagataagca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
    22381 cctatccaac taataaaatg caaacaactt ccggcaaaaa agtgatacaa gatcgatgac
    22441 atagcgttga agaaaaggat ttgataatcc gcaaaatttg cacaacactg cctgtaatac
    22501 ttcctgaacc agagacgatc atgaccgtaa cccgcccacg cgccgaacgc ggcgcatttc
    22561 cgcccggaac agaacattac ggacgttcat tattgggcgc gccgttaatc tggtttcctg
    22621 cccctgcagc cagccgcgaa agtggtttga ttctggctgg cactcacggt gatgaaaact
    22681 cttcggtcgt cacgctctcc tgtgctctgc ggacgttgac accttctttg cgccgccatc
    22741 atgtggtgct gtgtgcgaat cctgacggct gccagctggg gttacgggcc aatgctaatg
    22801 gtgtggattt aaacagaaac tttccggcgg cgaactggaa ggaaggtgaa acggtttatc
    22861 gctggaacag cgccgctgaa gaacgtgatg tcgtattact gacaggtgac aaacctggct
    22921 cagaacctga aacccaggcg ctgtgccagc ttatacaccg cattcaacct gcctgggtgg
    22981 tctcattcca cgatccactg gcctgtattg aagatcccag acacagcgaa ttaggtgaat
    23041 ggctggccca ggcgtttgaa ttgcctctgg taaccagcgt cggttatgaa acgcctggct
    23101 ctttcggcag ttggtgtgca gatctcaact tgcattgcat caccgcagag tttccgccca
    23161 tctcctccga cgaagccagc gaaaaatatc tctttgcgat ggcaaacttg ctgcgctggc
    23221 atcctaaaga tgcaattcgc ccgtcgtgaa ctgaagcgcc ggttccacat ctaccgccag
    23281 ccaggtcggt ccgtcaagat cggcgaaact gacctgcggc accagcggta aagcggcgct
    23341 aatggcacga gaggtacaca acatgcagcc cagcatcaga ctgaaacctt gtgcacgcgc
    23401 ttcagtcgcc agcgccagcg cttccgtcag acccccggtt ttatcgagct taatattaac
    23461 catctcatag cgccctttca gcgccttcaa attgctacga gtatgacaac tttcatcagc
    23521 acaaatcggc aacggatgaa taaaattctc cagcgccgca tcgtcctgcg caggaagcgg
    23581 ttgttcaagc atcgcaacgc ctaaatccgc caatagctgg caacgcgccg ccaacccttc
    23641 tgcacgccag gattcatttg catcaacgat cagcgtcgca tcgggcacag ctgtgcgaat
    23701 tgccaccatc cgctcactga taagatggtt atccagcttc actttcagta atttcgcgcc
    23761 tgcctgccag agtgttgatg cactattggc catctgatca ggcgtaccga tgacaaccgt
    23821 ctgtgcagtg ataactgtct cgggaagcgt tatgccgatc aaatcagcca gcgattgctg
    23881 ctgtcttcgc gaggccagat cccacaatgc acaatccagc gcattacgtg ctgcgccggc
    23941 agggagaatt ttttgcaact cctcccgtgt cagccctttc tctagttgcg gcacgacgct
    24001 cataatttgc gccattaccg aggcatcact ttccccataa cgcggatacg gcgtgcattc
    24061 gccggtgcct ttaataccct cttcttccag ttcaaccacc accacgcgcg cttcactgcg
    24121 acttccccgg gcaatcacaa acggggtatg taagggccag gcttcctcga ataccttaac
    24181 ggttctcatc tttactcctt gttgtcgcgg tggtctgcgg attaagcgca aatagcgttt
    24241 gctgtgttat tgacagttag cataaactag gtgtgacgtt aactatatgt aaacaggaaa
    24301 gataatcatg tcacaaaccg ttcatttcca gggcaacccg gttacagtcg ccaattccat
    24361 cccgcaggcg ggtagcaaag cgcagacttt tactctcgtg gcaaaagatc tgtctgacgt
    24421 caccctcggt cagtttgcgg gtaaacgcaa agtgctgaac attttcccga gtattgatac
    24481 cggtgtttgc gccgcatcag tacgtaagtt taaccaactg gcaaccgaga tcgacaacac
    24541 cgttgtgctg tgtatctctg ccgatctgcc gttcgcccag tctcgtttct gcggcgcaga
    24601 aggtctgaac aacgttatca ccctctccac tttccgtaac gctgaatttc tgcaagccta
    24661 cggtgtggca attgctgatg gcccactgaa aggtctggca gcgcgtgccg ttgtggttat
    24721 tgacgaaaat gacaatgtga ttttcagcca gctggtggat gaaatcacca ccgagccgga
    24781 ttacgaagca gctctggctg tactgaaagc ataatttaat atgcctgatg gtgttgcacc
    24841 atcaggcata ttcgcgctta ctcttcgttc ttcttctgac tcagaccata ttcccgcaac
    24901 ttattggcaa tcgcggtatg tgaaacgccg agacgttttg ccagtttgcg cgtgctggga
    24961 taattgcgat aaagctgggt taataccgaa cgttcaaaac ggctggtgat ttcgtccagc
    25021 gaaccttcca tcgcatcttc gcccacggct accgttgcgg cgtcataatc cggcaacaaa
    25081 atatcctgtg gacgcagctc ataaccgtcc agttgtgtca gtgcgcgata gatagcgttc
    25141 tttaactgcc gcacatttcc cggccacgca taacgcgtaa gtacagtatt caggtcagcg
    25201 gccagtttcg gacgcggcac gccctgctcg tcggcaaagc gggcgacgaa cagctcagtt
    25261 aacggcatga tgtcctgcgg acagtcacgt agcggcggca gattgagcgt caacacgttc
    25321 agacgataat agagatcttc acggaacatg cctttttgca ccagttcgac cagattcttc
    25381 tgcgtagcgc aaatcacccg cacatcgaca tgcacctcat ggtcttcgcc aacccgacgg
    25441 aaagtgccat cattaaggaa acgcagtaat ttcgcctgca tccgtggtga catttcccct
    25501 atttcatcca acagcaccga accaccgttc gcctgctcaa agaatccttt cttcccttcc
    25561 ggagcatgac caaacagttc actctcgacc gcatcttccg gtatagacgc acagttcagc
    25621 gccaggtaag gtttgcccgc tctggggctt gcctgatggc aggcgtaggc aaagagatct
    25681 ttacctgtac ctgtgtcacc cgtaatcagc agcggcgcgc ttagcatcgc cagtttctgc
    25741 gcctgttcga caacatgctt cattttcggg ctgacggcga caatttgact gaaggcgctg
    25801 acgtcctggg cggcgacatt ttgcaactgg cggcccatac gaatcgttga tcgcaacatc
    25861 accaccgcac cggtcaggac gtgttgatca ttttcatcct gaagataaac aggcgtaatc
    25921 tccatcagga aattctgccc attaataacg acatgctcgt tatgcgaatc ttgcggttcg
    25981 ctttccagcc aacgtaaaaa attaaagccg ttaatcaatt gtgcggcggt atggttgcgc
    26041 aggcgatcca atttttgccc aaaaagctga cagctcgccg ggttcgccat atccactttg
    26101 cttttcatat cgacagagag cacaggttca ggcaacgcct ccagtaacgc gctcaacgcc
    26161 agatgctcac gttcggaagg catccacggg acagtacgca catcggtaac acccgcaata
    26221 cggcgtattt cggccatcag actgctgaaa ctctcaaact ccagttcagc aaaattgagg
    26281 tagattcgcc caatgggatc aatctcaata ccgcgtaaat caatgcctct tagcacgagt
    26341 agatcgagta attcgcgggt cagaccgagt cggtcttcac aaaagacttc cagacgcatg
    26401 ggaaccttca cctgaaaaaa gaacaattaa tatgatgata tgacactatt tggatagcag
    26461 gaaggtttct gtcaacaatc attgacacaa agacggaaaa acgggcactt cggcgtaaag
    26521 atatccggtc agcacccgat attgcattat ttttcgctgg gcgttttgcc tttttgcagc
    26581 gtttctttca cctgaccgat aagctgacga cggaaatccc cgaggcgagg tttgtcatcg
    26641 tcaatccacg gcagcgggcg gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga
    26701 agtcctgcac caatcccctg agctgcgcgg gtagacaaac gagcagcgag atcttgcgac
    26761 atccagtcca tccccacttc gcgcaccagt tcgctggctc cggcaaaagc gatattcagc
    26821 aataccagct taaacagacg caaacggctg taatacccca gttcaatgcc atacagcgtg
    26881 gcgatgcgat taattaaacg cagattgcgc caggcgataa acgccatatc gaccaacgcc
    26941 agcgggctga ccgcaatcat caacgttgat tccgccgccg aacggctgat ttcgcgccgc
    27001 gcctgggcat ctaaaactgg ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg
    27061 ttttgcgttt catggattga ggcataccag cgttgcagcg ccggatgcga ctgatcaata
    27121 cccgcctgct gcgccagttt ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc
    27181 aataaatcac gcgcttcgtc gcgttcatgg gcgcgctgtc gcaagcgcca taagcgccgc
    27241 cactctgtta ccacagaacc tacgccagcg ccgataatca atgccccagc ggcacatcca
    27301 cccagcgcca cccagtcctg ggtttgccag gcattcattg tccactgtac accctgcccg
    27361 acaacgcttg cgccaaacag agccagcccg cccatcacca ttttgcgcca caggctgcgt
    27421 ttcggacgta acgctgcgtc cattaccgct tcgacttgcc cctcttcttc ctgcgcttcg
    27481 tcgagcgtgg ccggggcaaa attttgcgcc tgattttcgt caaaggtctg ctgcgccctg
    27541 aatttaggat tctgatcgac ctccagagga ccgtcgaaat caatacgtgg ttttaacggt
    27601 tcggtcatcg caatttatct cctattaaaa attccagcgc agcatcaaga cgaatatgcg
    27661 gtagtggttt gtcgacatcc atcacctgcg gacgaaatgc ctcaaactgg aagccttgct
    27721 tatcccagaa cgcctgacca ggcaaacgtg cgggaacttc gccaggataa acagtgagcg
    27781 gtgcgccatc gctaagtcga ttaccacgca gcgccgggat tttctcaccg ttaacatcaa
    27841 taatgccgct ggtggtcgcc tgaactgacg ccagccccag gcagtccatg ctgatccctt
    27901 cgaacgccgc attttgccag gcatcctgaa tcagttgttg cagtaatgaa accatattag
    27961 cgtgctgatc gatggtcaca tggtccgctt tagtggcagc aaacaatagc ttatcgataa
    28021 ccggcgaaaa caaacgccgg aacagggtac gctgcccgta gtgaaaactt tgcatcagct
    28081 gcgtcagtgc cagacgcata tcattaaatg cctgtggccc actgttgaga ggttgcaggc
    28141 aatccaccag cacaatctgg cggtcaaagc gcagaaaatg attcttatag aaccccttca
    28201 ccaccttctc gcagtaataa ttaaagcgct cgcgcagcat tccggcattg gtatgcttat
    28261 cggcctgcgc cagtttggac tcgccccagg tatcgacatc cggccacggg aagaattgca
    28321 gcgcgggcgc acctgccata tctcccggca agacaaagcg cccaggctga ataaagtgca
    28381 gcccctgctg tttacagtgg tggagataat cggtccacgc ggcggcaatg tccgccagcc
    28441 ggttttcgtc ggcaggcgct agcgggtcca gcccttcgct catcattcgc catttcgccg
    28501 accattctcc gcgctgacca ttgagtaagc ccgtcatctg gcgcgaccag cttaaatagt
    28561 cctgcgccag catcggcagg tcgagcaacc attcgccggg ataatccaca atttccagat
    28621 acagcgtgga ggtatcctta aagtggcgta gcagcgaatc attcgattta aaacgtagcg
    28681 ccaggcggat ttcactgacg ccgcgcgttg gcgttggcca ggcgggtgga tcgccatata
    28741 actgcgccag cccttcgtca taggtgaagc gcggaatgcc aaagtcacgc tggggaatgc
    28801 gtttcacgcc cagcaggcgc tcttcacgca ccgcacttaa cagcggcaaa cgtgctccgg
    28861 cgtgaatatt aagcaactga ttaaccatcg cggtgataaa cgctgttttg ccgctgcggc
    28921 taagtccggt tacagcgagg cgcagatgtc tgtcgacacc ccgattcacc agcgcattga
    28981 gttcattttt aagtcgcttc attgctgtcc tgtgctgctc tgaagaccag aaaaaaaatc
    29041 attcgtaagg atatgaatgt gggggccagg ccattgatta tcaactgccc gtgttgtcat
    29101 cgaccggcga actccataag atgtgcagca ttcacgttcg gacggagttc ctgacctatg
    29161 tcccctacta tttatgatat tgccagggtt gcaggcgtat caaaatccac cgtatcacgc
    29221 gtgctgaata agcaaactaa tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt
    29281 gaagaattac agtatcagcc aaacaagctg gcccgcgcgc tgacctcttc gggttttgat
    29341 gccattatgg tgatttctac ccgttcgacc aaaactacgg cgggtaatcc gtttttctct
    29401 gaagttttgc atgccatcac cgccaaagct gaagaagaag gtttcgacgt gatattacaa
    29461 acgtcgcaca atccggcaga agatctgcaa aaatgcgaaa gcaaaattaa gcagaaaatg
    29521 attaaaggca ttattatgct cagttcgcca gcggatgagt cattttttgc ccaactcgat
    29581 aaatatgata ttccggtggt ggtgattggc aaagttgaag gtcaatatgc ccatgtttat
    29641 tctgtcgata ccgataattt tggcgacagc attgcgttga ccgatgcatt aattgaaagt
    29701 gggcatcaaa atattgcctg cctgcatgct ccgcttgatg tccatgtttc ggttgatcgc
    29761 gttaatggtt ataagcagag cctggctgcg cataatattg cagtgcgtga tgaatggatt
    29821 gttgatggcg gttataccca tgaaactgca ttaaaagcag cacggcaatt attaagccag
    29881 tcaccgttgc ctgaggcggt gtttgccact gacagcctga aattaatgag catttatcgt
    29941 gcggcagcag agaaaaatat tgctattccg cagcagttag cggtggtggg ttatagcaat
    30001 gaaacgctgt catttatttt aacgcctgca ccgggcggca tcgatgttcc gacgcaggag
    30061 ttaggtcaac aaagctgcga gttattattc cgcttaattt ctggaaaacc gtcaccacag
    30121 aatattaccg ttgccacgca tatgacgctg aaataaatac cagctacctt gcctgaatgc
    30181 cggatgcgat gctagcgcat cttatccggc ctacatttat agatcggata aagtactcgc
    30241 gccgcatccg gcaatttagc ccattatcag aacgagtaat ttacgccgac acctgcataa
    30301 tggaatttat cactgtcgcc ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa
    30361 acggaaagtc cgttctggaa atcataaccg taaaataaac ctacacggtt aaaatcatgg
    30421 ccttcacgtt caatatcatc ctgccagtcc cagttactcc agcgatccag cccaatgcgc
    30481 gtatacggcg tcaccgagtg gttgccgagc gtcagcggca aataggcgcg aatttcttgc
    30541 gtggaaaact caccgttatt gcggctgtcg tccatattga agccgcgctc gagataatag
    30601 ttcactcgca aggcaaccgt ttcgttgaag gtatattgca gacctgtttc cgtttcgaca
    30661 cgggtatcag cgtaaccggt agtgttcaga tcgttggcaa atttatacat cgacaaccaa
    30721 ccgttgaaac gtaaatcgtc agtcagtttc acatcccagt ctggcgcgat tttccagcgc
    30781 tgcatattcg ccgtgtcttt acccggttca tcaacgtagt gataaccata attacggaaa
    30841 ccgccggtca ggccgaaact gaaatcatcg ttttcgagga actgataatg cacctccagc
    30901 tccgggcgat caaaccacgt tccacgttta cccgcgctat aatctaccgg cccttcctga
    30961 taataggcca gagcaattct ccacggcccg ttggcggcat taaaatagac tgaaggctcc
    31021 gccagcccat ccatatcttc gccataaccc tcgacgtttt ctatttcgta catcgcgccg
    31081 atattaaagt gccagtcgtt cctttcctcg gcctgtgcgc aggccattcc cgcacatatc
    31141 accagtgcgg tacagggtaa taactttttc attattgttc cttgtattat cctgggggaa
    31201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    31261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    31321 cacggaccac taactcgtgc cccccaaccg tggtgtagag cataaattcc gcaccggtta
    31381 attctgccac gctaattttg gcggaaatgt tattttcctc ttgcgcgtcc ggatgaatat
    31441 cttccggtcg tattcccatc acgatgggct tatgcaaact ttcctgtgtt ttcagaaccg
    31501 ctaatttctc ttcgggaatg gttaatttaa gcgtttccgt aacgaattta tcgccatcga
    31561 tcgtgccgcg aataaaattc atcgctggtg atccaataaa tccggaaaca aacatattcg
    31621 cgggttggtt ataaacggtt ttcggcgcac ctacttgctg aacaatcccg tctttcataa
    31681 tcacaatccg cgtcgccatg gtcatcgctt cggtctgatc gtgggtcacg tagatcatgg
    31741 tggtgttcag tttctgatgc agcttgctga tctctgcgcg catttgcacg cgcagcttgg
    31801 catcaaggtt agagagcggt tcatccatta aaaacacgcc cgcttcgcgt acaatcgccc
    31861 gcccaagcgc cactcgctga cgttgcccgc cggaaagcgc ccccggctta cgtttcaggt
    31921 actcacgcag gccgagaatt tgcgccgccc agttcacccg ctcatcaatc acctctttgg
    31981 cgattttttg catcttcaga ccaaacgcca tgttgtcgta aaccgtcata tgcggataca
    32041 acgcgtagtt ctggaacacc atcgctatat tgcgtgcttt ggctggaacg tcattcattc
    32101 gtttgccgtc gatcaacaga tcgccgccgc tgatctcctc aagcccggca atcatgcgca
    32161 gggtggtcga cttaccgcag cccgacgggc cgacaaacac gatgaactct ttatcggcaa
    32221 tttccaggtt gaagtccttc accacatgca cctggttatc gtagattttt tgaatatgtt
    32281 gtaacgaaag ctgagccatg ttgattcctt tgctatacgt tttgccagaa ggccgataac
    32341 cgcggccagg tgagtgattc cgttgaaggc aacagtaatt gcgccccggt taagcccgcg
    32401 ccgatcccca ccgagcgcat accgctggcg ttaatggcgt caatgcccgc ctgcgcatct
    32461 tcaatgccga tacatgcctg cggcggcacg cccagccctg cacaggcggc gagaaagatt
    32521 tccgggtccg gtttcgagtt tttaagttgg gaagcatccg cgcagaaggt gaaaaactcg
    32581 cgcagctcca gcgccgctaa aatcgtcggc gcattcaggg agacagaagc cagcccaacc
    32641 gagatctgct gtgcacggag atctgccagc aaagagcgaa tgccgggtag aacagcgttg
    32701 accgtcaact cgcgtagtga gtggacatag agcagatttt tgcgatacgc cagttgcgcc
    32761 ctctcctgcg agttaaagtc gccctctttg cccccgtgtt gcagaatgcg ccgcagagac
    32821 tcatcgcggc tgatcccttt tagggattcg ttaaactgcg catcaatgct gatgccaatt
    32881 tcagcggcaa tctgctgcca cgcctggaaa tgcagatgcg cggtatcggt gattacacca
    32941 tccagatcga aaattacccc ttgcagtttc attcatcctc ctgatgtttg gtagcggtcc
    33001 cattgaaggg caaaataaaa tcacccaaac agaaaacaga ttcttcagcc acggttataa
    33061 gctgaccgtt caaacgcagt gaaacgggcg cagaagttcg aatcgcaata cgctgcgcgt
    33121 caagagtgac ctgtaattcg cagccctgcc agaacagagg gaaagacaac tgttgccact
    33181 gctcaggtaa cgccggattg agatgcaatt caccgtcacg cacgctcacc ccggcaaaac
    33241 cctgaatcgc ccccagccag atagcgccag ttgcggcagc atggatacca tcatcacaac
    33301 tatgcggatc agcaccaaga tcgatttcag tcccctcgcg ccagaactga taactttggg
    33361 tcagcaggcc acagcgtgcg gcaacaatgc cgtggattgc tttacttaat gacgagtcgt
    33421 gaatagtgcg cggttcataa aattgcagat tggcaagaca cgatgccgct gagaactgct
    33481 ccggcagcat gtaattgagc atcaccacat cagcttgttt gaggatctgc atctcgttca
    33541 cttctgcgcg tgaataatcc agcagtatgg tttgcttccc cgccgccgct ttgtatttcg
    33601 ccagattaat cgccggctta gccataaacg aatcatcctg cggcaaaacg ccgtcgggct
    33661 gaatttctgg catccatagc tctttgagga acatttcggc gcgatggata aacgcatcgt
    33721 cgctacagcc gaactggcgg gcaatattca gcgcctgttg aacgttgtag cgggccatat
    33781 agctggtgta tgcattatta ttgacatgtt cggtatattc gtctggccca ataacatcat
    33841 gaatttccag acgatcgtta actctcaccg cgcggctaat ccagaacttt gccgtctcca
    33901 gaagtagcgc catgccttca tgcgcaatga aactttcatc ccccgtggtc tgccagtatt
    33961 gaataaccgc ccaggcgata tcggccacca gatgatgttc cgcctgcgcc gaggccactt
    34021 tttgccgcag cccggtgcga atgttaatgg cggcaaattc cggcgtctct tcttcgccgc
    34081 tgcgcgcgct ttcccacgga aatagcgcgc cctgccagcc gttgcgtcgc gctttctcct
    34141 gcgcgcctgg caagttgtgc cagcgataac gcagtaaact tcgggcaacc gtcggatcgc
    34201 taaacagatg aaacggtaac aaaaatactt ctgtatccca gaaaacgtgg cctttgtagc
    34261 cttcgccggt taagcctttt gccgcaatgc tgctgcgctc gtcgtgggca ggcgtcatga
    34321 tgcgcagatg ataaagcgca taatctaacg cttgctgatc gtgcgcttcg ccgccattta
    34381 ccgtgatacg acgtttctgc caccattgac gccagttttc tgttgatgct gcaagaagtt
    34441 ggtcgtaact ctgctgcgcg cacatttcaa gctggcgaag cgacgcgctg ccccactcgt
    34501 ctaaagcagc ttgcctgtca tcccgccagt cgatccacac cagtttttgc aacgtcattg
    34561 tctcgcctgc atgaagctgc gcactggtat gttgcagtaa acggcgctct ttggcggtat
    34621 agcattgctg cacatcaccg ctcaccttac agcaacagct gatggccaca tcactgcgtc
    34681 catcctgggt ggtgtagctc ccctgcatca gatgctgacc aaacacccgc acctgggttt
    34741 cgtcgagatg ttggcgaccg tgattggttt gcgtggcgtc aatgcctgtt gaaatcagca
    34801 ctgacgcgtc ggcgtccagt ggcgtaatag tgatttccag cgcaatgagc ggcagttggt
    34861 ctgccgaaac aaagcgacgg ctggcgatgg tgtaacctga gccgttgctg gtacgccaga
    34921 caacattgcg gcgtaattcg ccactggcaa agtcaagctc acgctgccag gcttcgtggg
    34981 ataacgagaa aacctcacca tttatggcaa tctccatccc cacgacatca ggcaggttca
    35041 ccagttcgtt gatttcacct tttcccgccc gatgatacag ccccgccaga tacatccctc
    35101 gcgtctggcg cgtgtaatct tcttcatggc tggcgcgaag cccaagatag ccgttcccct
    35161 gcgccatcag cgatgcatac ttgttcaggg tatgctggct gaaatggggt tctgataacg
    35221 ttactggcct ggtcataatt ccacacgcgt ccctgtttct gcggattgat acaacgccgc
    35281 caccagttgc tggatgatgt acccctgctc ggcgtcggct atcatcacgg gcttgccctg
    35341 tacgtgattg ataaaggctt ccatgctgcg caaatggcgg ttgtcgtctg ccatttcccg
    35401 ttgcatcagc gtcattaatt caccgttgtt atcggtgtag atatgtgctg gaaacagcgt
    35461 cgcaccagct ttatcaccac aaaagctgac gttcataatc gactgttcgc ggatgttgag
    35521 tgcaaatgac gtttccagcc acagaatgcc gccgttatga aattcaatgg tgccaaacag
    35581 cgaatcttcg acgctgtaag ttgccggatc ccactcacca aattgaccac agctcttttg
    35641 cgtgccgatc ttttgaaagc tatgcgcatt cacgcttttc accgccggaa agcccagcac
    35701 atacatcgca gcatccagca tatgaatgcc gatgtcgatc agcgggccac caccctgcag
    35761 ttctttattg gtaaagacac cccagccggg aacgccgcag cgacgcaggg cgcgggcggt
    35821 ggtaacgtaa atctctccca aaacgccgtt ggtcacctgt tcacgcagct gttgcgtatc
    35881 gagcgcaaaa cgatggtgaa agtcgtaggc cagcaccttg ccctgtttgc gcgcggtatc
    35941 gcacatttcc cgcgcctgac ctggcgtcat ggcgggcggt ttttcgcaca tcacatggca
    36001 gcccgcttca agcgccatca gggtatgttc gtaatgaaaa cgattaggtg agcagacgct
    36061 aaccacgtca ggtttcaccg ccagcagcat ggcctgcgga tcgtcccaca cggatgcatt
    36121 cccgtatttt tctgccagcg cctgcgcctg ggaaaggcgg ctgtcacaga cagccaccag
    36181 ttccagatcg ttgcgggtgc agtagtacga agcatggacc ttatccgcaa cctggcctgc
    36241 gcctattatc gcgacccgca gcggagagct tgtcattgca cttttcacaa agacctctta
    36301 gcaggtacgc aaccaggcca acgaatcacg gtacgcctgg gcaggatctt ctgcccggat
    36361 acgcccttca tacaccacat aaccctgata gttatcggcg cgcagctgtt caaacagcgc
    36421 gtggaaatcg agggtgccgc tgcccggctg gtaacgatgg ttatcggcaa tatgcacatg
    36481 accgagcaag tcacggttgt cgtgcagcgc ctgcgccagg ttatcttctt cgatgttcat
    36541 gtgatagaaa tccccgataa tctgcacatg tttaagattg ttttcgacga tgtaacgacg
    36601 agcatcggcg agggtgttga tcatatgatc ctgatagcgg tttaacggtt cgagatacac
    36661 cacggttccg gtacgcgcgg cgacctgctc cagtacgcgc agggaatcac tcaccatttt
    36721 gcggtcgccg tccagactac gcggtgaagt catcggcggt aagcggaagg taaacatgcc
    36781 ccacgctgcc ggaacgacga tacctttacc gcccacttct gccagcgctt cgagaatgcg
    36841 ttcgatctgc tttaagccat taagacgacg ctcttcgata aagtcaccaa tccagccgtc
    36901 atagccaccg caggcggtgg tcaccggtaa accggtttct ttgatcgccg ctttgacttc
    36961 ttcgatgttg ttgaccagca gtttgccgtc aatctcaaaa ccatcgaagc ccatctcttt
    37021 gatataacga aatttctcca gaatgttttc cggaaagaac gcctgattct gtgtgccgat
    37081 tttcattttt ttcattcctt tgaattaaaa cgtaacgccc attttgatgc tctgttccgg
    37141 atgctggtcg acatactgca tatagctttc cgggctattg gcaaaggtca ccaccgggtc
    37201 gattaaatct tcgcaattga gataaccgtt catcagcagt tcccaacagg tttcttcaat
    37261 acgcttgcgg ctccagcgcg gataatccgg gttcggttcg ctgcacgcgc gagagaagac
    37321 aattttggcg ttattgaaat gcgcttcgcg tccgaggtta aaaccttcgg caaacggctt
    37381 ggcaaacgcg acataggaga tggtgccgcc ataagccaga ccgcggagcg ccgattgcag
    37441 cgcgtcggcg tagccgctgg tttcgataat cacatcggca ccctgcttgc cggtcagcgt
    37501 tttgatctct ttacctacat cagtgccgat ggggttaagg cagaaatccg cgccgtggcg
    37561 acgggcaata tcacagcgat gggcgatagg atcgacgcca atcaccacag aagcgccagc
    37621 gcgtttagcc agttggatgg caatttgacc gattgcgcca agccctacca ccaccacaaa
    37681 atcccctacg cgcacgttgg catcgcgcac gccgctcatg gcaaactgcg ccgggtcgta
    37741 gcagacggcg tttttccagg agctgccttg cggcattttg cgcagcttgt agttattcac
    37801 tgcgttaatg atgaccgtct cggagagcgg gccgtagccg catacgctgt cgcccaccgc
    37861 gtagtcggta acgtcgctgc cgcactcgat aatgtcgcca accaccatgt tgccaagctg
    37921 gaatttgcca aactcaatgc cgcgcggcgc atctgccgga cgcggagtga acatctgcca
    37981 ttcgccgtta aagtcttcat caataaacgg gctggcggcg cggaagtcga ccacttccgt
    38041 tccgtgtttc ggtgcgccga aacgcacgcg gatcttcact tcattagcta aaatcgcccg
    38101 atcttcatac tcaaccagcg cagcaacacg cggtgctgtg gctactaact ttttcattta
    38161 aaactccttg taaagaaact ggccgcttta gcccttcacg ccaccggcgg tcaaaccact
    38221 cttaataaaa cgttccgaaa gcgcatacat aatcaccacc ggcaatgcgg tcaccagtga
    38281 ggccgccatc atccgtcccc agatgtaatc tggcgtactg aacagcgcgt tcaggcccac
    38341 cggtaaggtg aaattgctgg cgctggagag gaaaatcgac gcaaacagat agtcgttcca
    38401 cgccaccata aagcagtaga cgaacacgga tatcagcccg gacatcgcca acggcacggt
    38461 gatgcggaag ataatttgca gacggttgag gccatccatc atcgccgctt cttcgatctc
    38521 atcggggatg gtgtcgaagt agcttttcag cataaacacg gcggtgggca gagtttgcgt
    38581 caccatggtg atgatcagcg ccatctcagt gtcgtaaatg ccaagcgcgg tgatgatttt
    38641 gaaaagcggc accaccagca aaatgccaga gaacatatac gccgtgtaaa agctggcgtt
    38701 gatggtcatc cgacctttaa agcgcaggcg ggaaagcgcg taagccccaa gaatgccgag
    38761 gaataccgcc accacggatg aaaccacaga caccaccaga ctgttacgga agtagtcgac
    38821 aaacgggaaa atcatcgggt taaaaatgtc gacgtaatgc tccagcgtcc actgctgcgg
    38881 cagcagcgta ggatgcagtg agatcgcctc tttcgcgccc ttgaacgagg tcatcagcat
    38941 cacaaaaaat ggaaacagcg tgatgatgag aaacagcgcc agcccgcagt aaaaaccgat
    39001 gcgactgagt gtgcgtttat ttgttgccat tgaggttcac ccgtttcctg gtcagcagaa
    39061 tgacagcgaa aataatgatg aagagcacca ccgagatcgc cgccgctttt cctaaatcat
    39121 taaaggcaaa ggcggttttg tagagatata caccgagaat atcgaccttg gtcgtcagca
    39181 aataaacatc cgcgaacatg tagaacatcc agatggtgcg cagtgtcacc accgtcgcca
    39241 ggaccggcat aattgcgggc agcgtgacga tacgaaaccg ttgccaggca ttagcgccat
    39301 ccatctctgc cgcttcgtac agcgatttgt cgatggtctg caaaatcgcc agaaacgaga
    39361 taaaggcata cgggaagtag cgccagatgg cgaacagcac caccagcaca aagctactgc
    39421 ccggattatc gaaccacagc ggtgcctgct catagagatg cagaagatcg acgccgaggt
    39481 agttaacaat gccgtagccg ttgttgaaca tgtatttcca ggcgaacacc agcgaaatgg
    39541 acggcgttac gtaggagagg atcaccagcg aacgcgcggt tttgcgcagg cggaactcgc
    39601 ggttgaaaaa catcgccacc gccagcccga gcacggtgct gcccgccacc accagcgcgg
    39661 tataccagac cgtcatccac agcgaatgcc agaagccggg atcggagagg atacgcacat
    39721 agttgctcac cccaacaaac gttgactcga tgttgggatt gagcggcaga cgtaaaaaac
    39781 tgatttcgat attcgacacc atcggccacg ccaccagacc gcccagcagt aataagctgg
    39841 gcgcgagaag cagcagcgca aagggcatat cggaacgacc tgaaaaaagc ctgttcattg
    39901 cggattcctg tcctgggttt agtgctgttc gatcagttca tccagttttt tctggctcgc
    39961 ttgcagcgta gtagagagat cggctttacc cacggtgacg ttatgcacca ttgaactcac
    40021 cacgccagaa cccgtcacat cacccatgcg ggtaaagttt ttatccccta ctgcgccaaa
    40081 aacctgaata tttggcagtt caccgattag ctgattcggt agttcaccca gcgccttaat
    40141 aacgtcgttg tctttccagg tggcggtagt caccaccgct ttattcaccg gcagcgcagc
    40201 acctggcgac atcatcaccc agtcggcaat gttgtctgcc tgctccataa aggtgacaaa
    40261 tttttctgct gcttccgtct cttcggtctt ttgcccggcg gtaatggtca gcgaggtcaa
    40321 catgccgtag accgcagagt ttttctcggt tggcaccacg aaaccgacgt ttttcgggtc
    40381 gccttcttta atcacagccg gaaggatata ggtggagtaa atcgccatcg gcgcggtgcc
    40441 gttcataaag gcgtctttca cttccatgat gtcgttagaa cccggcatag tgttggcagc
    40501 aaggtcgcgg taataggtca gtgcctgcat catctctggt gtatcaaggg tgattttgcc
    40561 ttcggcgtta aagacgttag cctggttgga taacgcaaac tgggagaagg attgttccgt
    40621 caacacgctt tctgctgtag gcagcgcaat gccgtatttt ttattcgccg ggtcattcag
    40681 tttctgtgca acgtccagca gctgttgcca gtttttcggc tcctcaagcc ccgcttttgc
    40741 cagcacatct ttgcgatacc agataccgcc aatccaggcg ctgacaggaa caccggtcca
    40801 tgcgctacca tcttcggtac gcacaatacg cagtacgcca tcgtaaaacg cgccttcacc
    40861 aacgttgctg atgactgtgg caaccgcttt gcgatcgata agctgctctt tgtccatcac
    40921 tttggcgtag tcatggctgg tttcgatcac ttccggcagc gaaccgctac gtgaaagagt
    40981 aatgacttta gtgttatagg catcttcttc cacgggcact tgcttaacgg tgatgccagg
    41041 gttttctttt tcaaaacggg caatcaattt actgataacg gcctggcgct cctgctccac
    41101 cgaagaatgc ataaattcga tggatacatt cgttttattt tcttctttac agcctgaaat
    41161 cagggcgcat gaaaccagtg ctgataacag cacgattttt gatttaatca tgagatgttc
    41221 ctttttattt aatccacata acctgccagg ggcgcagcgt tatttcatta cccaatatat
    41281 caacttcact aatcagatcc cgaccatgca gattagtaat atttacatgc tgaatatttt
    41341 tactgacatt aaacaatcca gtcagacaat taccatcagc gttacttctt tgaatacgca
    41401 ttacggatga attaatcgta tcaatggtaa aattattatc cggatgaaac tcgttatggc
    41461 tgcgacgaag tgtaattaaa cgcgacaact catgatatac cgcatgcctt aatgtagctt
    41521 catcgttcag ttctcgggtt atctctttac tgtgatattt tttacggtta atcgcacggt
    41581 tatatccgag tttttcgaca cctgcgtaat cattacgcga gccaagaata ctttgaatat
    41641 atatcgctgg cacaccgggg aaacttaaca aaatcgcatg ggcaaggata aacctggcgc
    41701 aacgttcttc atcgctactc tcacggcggc ttaacgcatc catataggtc acatttattt
    41761 catacggact gcgtgtaccg tcgggattat ttttccagtt tactaatgca ccttcctgct
    41821 gtaacgcctc gaccagctct aatatttcgc tttcaggcaa caagccccgt agcgggttta
    41881 gcccgatgcc atcgtgagag gcgaggaagt taaaccaggt ggtgttgctg gaaggtagtg
    41941 tcaggttttg cgcccacgca caaagcgcct caacgttctg tttttgcacc gcatgcagca
    42001 ccagtggcgg cagcgagaac tggtacacca tatgtgcttc gtcatcgcct gcgccaaagt
    42061 aagcaatgtt gtctttatgc ggaacattgg tctcggtaat gatcactgta cctggcgcaa
    42121 cgttatcaat aatcgaccgt aacagtttga taatcagatg tgttttttcc agatggatgc
    42181 agcttgttcc cggctctttc cacataaagc caacggcatc caggcggaca tactcagcac
    42241 ctttcgcaag gtaacagagt aaaacatcca ccatcgccag caacacttca gggctacggt
    42301 aattcaggtc aatttgatcg tcactaaagg tggtccataa atggcgcgtt gaatgatcgc
    42361 gcatctggaa tggcgttaat aacggtaacg cacgcgggcg agtgacggcg ctgagatcgg
    42421 tttgcgggtc aacggcaata aaaaaatctt caaaacctgg atgctgttgt aaatagtttt
    42481 taaaccattc actttttgcc gacatatggt tgcagacaaa atcaaacatt aaatgactgc
    42541 attcaccgag ttgctgaata tcctgccact cccccgcttc actggcgacc tgatgataat
    42601 caattaccga aaagccatca tcagatgacc atggataaaa cggcaacaaa tgaacatgtg
    42661 aaaaaatgct ttgcagccat tgatggtaaa actgattaaa tgtgggtaat ggttttaaat
    42721 cattgctgtg aaattgatcg gcataggtaa ttaacacgac atcactttca tcccagtgtt
    42781 ttttacgtcg ctgtgtaatt aatcgtttcg cactctcaag acgcgttaca agtttctgta
    42841 aatgagttgc ggtaaatgtt ccaccgtaga tttcgtccag gtaatccgta attttctgtt
    42901 tcatgttgcc tttccgtggt agcggtccca tgaaacgaga gactactccg cagaaaataa
    42961 actgtcaatt gagcacaagg gttttagtca cgcaggaaat cattttttac cagggaaaaa
    43021 gcgtatttgt gagctgacgc aaataatcta tggggatgta aataaggtgt gacagaaaaa
    43081 aaaacggcgc ataagcgccg ctcatggtga attcttttaa cctttgacct tcggcattgc
    43141 gatgtctttc aggccaccgg cgttctcaac gtgggtatat cccatctcgc taaggatctc
    43201 ttttgcttgc cctgactggc gtccggcatt gcaatacact ttcacggtgt cgtttttatc
    43261 cggaacggcg gtggcaatgc gctctttcac ttccttcagg ggaatattga tggccccctg
    43321 aacgtgctct tgctgatact gctctggaac acgaacatcg atccagtgtt cagcggcgaa
    43381 aacgggcagt gaaaacacca gtgccagagc aagtaagcct tttttaaaca tgtaagcgtc
    43441 cttctatgga tgaaaattgc catccattat acgcctgaga tttaacaatt tttgcgaaac
    43501 tcccctcacc ttttataacg ctgtgccagt ttattagcag cccgacttaa cagcggttcc
    43561 agcgccacag ccagcaacat tttcagcggt cggcgagcaa ctgattttat cgcccagccc
    43621 gccacacccg ccgggccata gcgcagtgcg gtaagaagta ccagcttgcc tgctaatttg
    43681 aaaccaggct ttaccttttg cccggcctgt tgccagcgag tattcataac tttcctcaca
    43741 gttgacggaa acggctacgt aacgtgaaag tatcggaagt gacataacgt tccatctcgc
    43801 gtaaacgcgt ttcacttgcc gccagttcac ggtcgacttc atccagcaat tcgctgctgg
    43861 aaggtagctg ctcaccaaag gccatgttgt ccggcattgg atcaagcgca aatgacaaaa
    43921 tgatgtaagc aaccagggta aacagcgcca gaccgaagaa aatcgacagc accaccagga
    43981 tacgcaccag ttttaccggt acatcaaaat agttggcaat cccggcgcag acgccgcgga
    44041 ccatgccctg ctgtggaata cgccataatt ttttattgag attaatgccc gccattagcg
    44101 atccctccag ttcggatgtt ctgcatcaag aatagattcc agcgcctgaa tacgttcgcg
    44161 catccgtttt gcttcatcag ccagttgcgc taatcgctgc tgctcacttt gcgacaattc
    44221 actgcgacca gaacgattgc tgtaatgcag ccataaccag atcggtaaaa caaacagcac
    44281 aaaaatggtt aacggaatag ccagaaatag cgcgctcata agtactcctt agacgatgag
    44341 cggcggcgcg ttcggacgcc gcctggatgt tattattgat tgtcttgctt cattttggct
    44401 tttaattgtg ccagttgttc gctgattgca tcatcggctt tcagttcggc aaactgatcg
    44461 tccagcgatt tttgtttacc gaagctgtgg ctttctgctt ccgcttccat ctggtcaata
    44521 cgacgttcga aagattcgaa acgagccatt gcttcatcca gtttgccact gtccagctga
    44581 cgacgcacat cgcgcgacga gtttgccgcc tgatgacgta acatcaatgc ctgctggcga
    44641 gcgcgtgttt cgctcaattt gttttccagc tcgccaatct ctttcttcat gcgtgccagc
    44701 gtatcgtcca ccagcgtcac ttcatgttcc agggacttaa tcagatcggt cagtttctgt
    44761 ttttcaatta acgctgcacg tgccagatcc tctctctctt tcagcagcgc cagttcggct
    44821 ttttcctgcc attcaacctc acgcgccgac gcttgttcaa tacggcgagt cagctgtttc
    44881 ttttctgcca acgcgcgcgc cgaagtagaa cgtacttcaa ccagtgtatc ttccatctcc
    44941 tggatcatca gacgaaccag tttctgtgga tcttccgctt tctctaacag agcgttgatg
    45001 ttggcattca cgatgtcggc aaagcgagaa aaaataccca taatgttgtc ctcttgattt
    45061 ctgcgttcag gattgtcctg ctgaactgtt aatacaattt gcgtgccaat tttttatctt
    45121 tttgatttat aaagatctga ttgaagaatc atcagcaaca tgccaggatg aattagcgaa
    45181 ttacactaac aagtggcgaa tttcatcatg gcagaataca aagataattt acttggtgag
    45241 gcgaacagct ttctcgaagt gctggaacag gtttcgcatc tcgcaccgct ggacaaaccg
    45301 gtgctcatca tcggcgaacg cggcaccggt aaagagctga ttgccagccg cctgcattat
    45361 ctctcctccc gttggcaagg gccgtttatt tcccttaact gcgcggcgtt aaatgaaaat
    45421 ctgctggatt ccgaactgtt tggtcacgaa gcgggggcgt ttaccggtgc gcaaaaacgt
    45481 catccaggga gatttgaacg tgccgacggc ggtacgctat ttcttgatga actcgctacg
    45541 gcaccgatga tggtgcagga gaaattattg cgcgtgattg agtacggtga actggagcgc
    45601 gttggcggca gccagccatt gcaggtgaat gtgcggttgg tatgcgcgac gaatgccgat
    45661 ctcccggcga tggtcaatga aggcactttt cgcgctgacc tgctcgaccg actggctttt
    45721 gatgttgtac aactgccacc actgcgcgag cgcgaaagcg acataatgtt gatggcagaa
    45781 cactttgcca tccagatgtg tcgggaaatc aagctgcctc tgttcccggg gtttacggag
    45841 cgcgccagag aaacattgct gaattatcgt tggccgggaa atattcgtga attgaaaaac
    45901 gtggtggaac gttcagtgta tcgccacggc accagcgatt atccgcttga tgacatcatt
    45961 attgatccct ttaaacggcg tccgcctgaa gacgctatcg ccgtttcaga aaccacctcg
    46021 cttccaacac tgccgctgga tttacgtgag tttcagatgc agcaggaaaa agagttgctg
    46081 caactcagtt tgcaacaagg gaaatataac cagaagcgcg cggctgaatt actggggtta
    46141 acttatcatc agttccgcgc gttgttgaaa aagcaccaga tttagcgaag ttgagcaata
    46201 caacttgccg gatgcggcgt gaatgcttta ttcggcatac ataatcaata cgtcgtttgc
    46261 aggcctgata agcgcagcgc atcagacatt attgcgttgg gctattaatc gctcagcgca
    46321 tcctgcaaaa ttttcattgc cgcatcgaat tgcgcatccg ggatggtcag cggatacagg
    46381 aagcgaatca cgttgccgta tgcgccacag gtcagcagga gcagcccctg cgccagcgcg
    46441 cgttgctgga ttttctgtgc aatcgccgct gacggctcgc ccgtttgcgg atcgttaaac
    46501 tctgccgcaa tcatagaccc cagaccgcgt accgccgcga tggccggaac gctttctttg
    46561 gcatcaatca gcgtgtttgt cagacgctgt cccagttgat tcgcgcgttc gcagagtgat
    46621 tctttgtcga taatgttgag caccgcgtgc gcggcagcca ccgccagcgg gttaccggcg
    46681 taggtgccgc caagcccgcc cggcgcgggt gcgtccataa tattcgcgtt accgaccacg
    46741 cccgaaagcg gcatcccgcc cgcgaggctt ttcgccatcg tcattaaatc cggcttatcg
    46801 gcgtaatgat ccatggcaaa cagcttaccg gtacgcgcaa agccgctttg cacttcatca
    46861 gcaatcatca caataccgtg ctcgtcgcac aggcggcgaa tagcggcaac cagctctttt
    46921 ggcgcaacgt tgaaaccgcc ctcgccctgc accggttcga aaataatcgc cgccacctgc
    46981 ttcgcttcga tgtctgattt aaacaagcgt tcgatggcgt cgagggagtc ctgtgttgaa
    47041 atgccgtgta aatctgacgg ataaggtacg tgatacaccg aaccagggaa cgggccgaag
    47101 ccgattttgt acggcgcaac ttttccggtc agcgccatgg tcatatacgt acgaccgtga
    47161 aagccgccgc taaacgcaat cacgccaggg cgtccggtat gggcgcgagc aattttcacc
    47221 gcgttttcca ccgcttccgc accggtggtg aagaacgcgg ttttggcctg cccgctcacc
    47281 ggggcaaggg cgttgatttt ctccgccagg gtgacgtagc tttcatacgg cacaatctga
    47341 tacgcggtgt gggtaaactg ttgcagttgc tgctccaccg ccgcgaccag atcagggtgg
    47401 cgatgtccgg tattcagcac cgcaatgcct gcggcgaaat cgatgtactc gttgccctca
    47461 acatccttca gcgtggcgtt ttcagccgac tgggcgaaga agttacacat cacgccaacc
    47521 ccgcgcggag tggcagaaag acgacgctga tggaattcat tgttgctcat gtttcacacc
    47581 ttttgactct tcacggttaa tcggaaatta aacgcccaga cgatcgcgca ggctgtaata
    47641 cgccgcgccc atcgcggtaa acggcacacg cagcgtgcgc ccgccgggga acgggtaatg
    47701 cggcagattg gcgaaggcat cgaaacgttc ggcgtcgccg cgcagcagtt cggcaatcaa
    47761 acgtccggct agatgagtac aggtcacgcc gtggccgctg tagccctgca tgtaatagat
    47821 gttggtatcg aggcgaccaa actgcggcat acgcgacagg gtcagcagga agttgcccgt
    47881 ccagcggtaa tcaattttca cgcccttcag ctgcgggaag gttttcagca gtttcggcac
    47941 cacaaggcgc tcaacgtcat ccgggtcgcg cgcgccgtag accacgccgc cgccgtacag
    48001 caggcggttg tcggcggtaa gacggtagta atcaagcaga tagttacagt cttccacaca
    48061 gtagtttttc gggatcagcg aacgggctaa atcttccgac agccgttcgg tggtgatcac
    48121 ctgcgtgccg cacggcatgc tgcgtttcgc cagttccggc tctactttat cgcccagata
    48181 cgcattcccg gcgacaatca catacttcgc cgtcacctga cctttggcag ttcgcacaac
    48241 ggctggcgtg gtgtgctgga tctgcgtcac ggcagaaagt tcatacacgc gcccgccgtt
    48301 gaggcggatg gcgtccgctt cgccaatggc aaggtttagc ggatgaatat gcccaccgct
    48361 gtgatccagc agcgcaccgg tatagcgatc gctggctact tcacggcgaa tggcgttggc
    48421 gtccagcaat tccagctgtt tattgccgta gcgttcccag ttctctttct gctcttcaag
    48481 tgtggcgagc tgtttatcat tcatcgccac aaacaggccg ccggggcggt agtcgcagtc
    48541 aatctgataa cgtttgatac gttcgcggat gatctcacca ccttcgaaca tcatgctgcc
    48601 gagcatacgg gcggtgtcca tgccgtagct tttttcgatc acgtcgatgt cgcggctgta
    48661 ggagttcaca agctgtccgc cattgcgccc gcttgcgcca aagccgatgc gtgaggcttc
    48721 gagaaccact acgtcaaagc ccgcttccgc cagatgcagc gcggaggaga gcccggtata
    48781 gccgccgcca accacgcaaa cgtcgcaggt gattgactca ttcagcgtgt cgaatggtgc
    48841 atatttattc gcactggcgg cgtagtaact gctggtatgt tcggtcattt caggcctcca
    48901 ggcttatcca gatggttttc agttcagtga atttttcaag ggcatgcagg gatttgtcgc
    48961 gaccgttgcc gctctgctta tagccgccaa acggcacggt catatcgccg tcgttgtagt
    49021 tattgacgaa gacggaaccg gctttcaggc gtcggctcat gcggtgcgcg cgggagaggt
    49081 cgcgcgtcca taccgccgcg ccaaggccgt actggctgtc gttggcaagc tgtagcgcct
    49141 gttcttctga tgtgaaacgc gtgaccacca gcaccggacc gaaaatctct tcgcgactta
    49201 aggacgcatt cgggtccaca tccacaaaga tggtcgggcc gatggcggca gccagcccgg
    49261 cgttacggcc atccaacaac agttgccctt tgctttcgcc ttcccgaata aagctatgga
    49321 ccgagtcggc gtgggcgcag tcgattaagg tgcccatggt ggttgcggga tcaagtggat
    49381 ggcccggctg ccagttttgc gcctgctgtt ttaacagggc taagaattca tcggcgatgc
    49441 tctcttccag caacaggcgc gttccggcga tgcacacctg tccctggttg tagaaaatgc
    49501 ctgctgcggt ggcgcttgcc gcctgttgca aatccgggca gtcagcgaaa acgatgttgg
    49561 cgcttttgcc gcccgcttcc agccagacgc gtttcatgtt gctgtcgccc gcatctttca
    49621 gcagctgttt cccggtacgg gttgaaccgg taaaggcaat ggcgtcgata tcgttatgac
    49681 gcgacagcgc ctgcccggct tcatgaccaa aacccgtcac cacgttcaac acaccatccg
    49741 gcaagcctgc ttctttcgcc agccccgcga gacgaatcgc actgagcggt gatttttcag
    49801 acggttttag aatcacgctg tttcccgccg ccagcgccgg gccgagtttc cagcaagtca
    49861 gcaacagcgg gaagttccac ggcacgatgg cggcaatcac gccgaccggt tcacgcacga
    49921 tcatcgccag ctcatggcta ctggtggtcg ccacttcgcc atacactttg tcgatcgctt
    49981 cggcgtacca gcgaatggcg cgcgccgcgc cgggaatatc atcacgcaga ctgtgacgaa
    50041 tcggtttgcc ggtgtcgaga gtttccagca gtgccagctc ttcggcgtgg gcttccatta
    50101 aatcggcgag tttattcagt accgctttac gtttagccgg agaagagagt gaccagtcgc
    50161 cgcgttcaaa tacgccgcgt gctgcgctca tcgcacggtc gatatcgacg ctcttgccgc
    50221 gggcaatttt cgccagcggt gcctgggtga ccggatcaac ggtttcaaag gtttcatttt
    50281 ccgccgcagc agtatattca ccgttaataa ataagcggtt ttcaatggcg agacttaacg
    50341 ctttatcctg ccagtaagcc agatgatgaa aattcattat gactcctgtt tcacgtctat
    50401 cagatatatg cagtttgtga acgtcagggc gcagagatcc ccgggccgta tgcccgttta
    50461 tttctggcga cattctttat atacacttca tccttcaagc cgcctctgcc ttggctgcgt
    50521 tcgctccccc gaatcactta cctgagtaag ctcatcggga ttcgctcact tgccgccttg
    50581 atgcaacttg aatgattttg tgtatagatt gccgggcgca ttgcgcttac ccggcttaca
    50641 gaacaaaaag attaaaacgt ggtgggcgta tgggcgctga taattcggca aatacctgcc
    50701 gacgtattac tgaaactgtg cgggatgccg gtattaatgg cataactttg ccccgcgacg
    50761 aggtggtaat cctgaccatt aatcgtcaga acaatttcac cttccagtac agtgcctatt
    50821 tcctcaccct gatgcttaat tctttcccca gtggttgtgc ccggctggta cgtttcaaag
    50881 atcatcgcca gcgtgcgatt cgggttaccg ttatgaacca gcttcattga cacaccctga
    50941 ctacccatct caattaagtc gtcctgatta atgacgacct gcggctcatc aggtttttcc
    51001 ggctcggaaa agaattccga gagtgacaga ccatacacct tcagcagctt ttgcagcgta
    51061 ctgatggcag ggctgacttt atcttgttct atcgtactga tagcactgtg agtcagcccg
    51121 gagagttcgg cggcacgacg ttgtgaaagc ccctgctgct ggcggatttc cgacaagcgt
    51181 tttcctggcg ccagtccctc atcactcata tttgcatttc cttaaactgt agtggtcaga
    51241 gtcgctgttt ttcagcgata tggtgctgac aagcggtgat aaagccctcg aacaatatac
    51301 gcgaaagcgc gtactcgcta ctgttccatt ccgggtgcca ctgtacgccc agcgcaaaag
    51361 gatgattgat gacgctaacc gcctcaacta agccatccgg cgagcgagct tcaacacgca
    51421 accgtgggct aacgaccttc gccccttgtc catgtagaga gtttacccaa aagttgctac
    51481 attcaggtaa caacgcagac agtaatcccc cctcttcaac ctgaacttca tgcgacggtg
    51541 catattgctg ttccaccggc agttcgggat cttcccgatg ttccagcaat tcaggctgtt
    51601 cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac cagttcttgt aaaccccggc
    51661 agatggcgaa aatggggatg cgcctttcga gtgcggcatt aattatcgcc atgctcagaa
    51721 gatcacgccc gggatcggcg tcaggctcat cgccgttttc accatatagg tgcggctgca
    51781 cattgctggg actaccagga agataaatgc catcgagttt cggcaaaagt tgttcaagta
    51841 atgacggttc cgccagcgca tgtggtagcg caataggcaa gccgcctgca tggatgatgg
    51901 cattcaggta cttttcttgc agagtctggg tcgcatgacc cttaagcctg ttcctgcaca
    51961 ttacgacacc gataaccgga ttgttcatta tattttccat gctcaatctc acaaagtgga
    52021 ctaaattatc gccattactg cgctttaaat gacctttatg ttcaatattt tttcaatcta
    52081 gcagtgggtt ttcatttttg caaactaaat ttaacatttg acaaacattt agtttgcata
    52141 cagattcgaa tggtggtcat tatattttac gctttgataa cgagcggaaa acaaaccaaa
    52201 ggcgaagaat catggaaacc aatatcgttg aagtagagaa ctttgttcag cagtcagaag
    52261 agaggcgggg tagcgccttt acgcaggaag tgaaacgcta cctggagcgc tacccgaata
    52321 cgcaatatgt tgatgtttta cttaccgatt taaatggttg cttccgtgga aagcgtattc
    52381 ctgtttcaag cctgaagaag ctcgagaaag ggtgttattt cccggcctcg gtatttgcaa
    52441 tggatattct gggcaacgta gtagaagaag caggtctggg tcaggaaatg ggcgagccgg
    52501 atcgtacctg tgttcctgtt ctcggttcct taactccttc tgccgccgat ccagagttta
    52561 tcggtcagat gctcctgacc atggtcgatg aagatggcgc tccctttgac gttgagccgc
    52621 ggaacgttct taaccgcctg tggcagcagc tgcgccagcg cggattgttc ccggtcgtag
    52681 cggtagagct ggagttctat ttactggatc gccagcgcga cgctgaaggg tatctgcaac
    52741 cgccctgcgc gcctggcacc gatgaccgca atacacaaag ccaggtttac tccgttgata
    52801 acctcaacca cttcgccgac gtgctcaatg atattgatga actggcgcag ttacagctga
    52861 ttccggcaga tggcgcggtc gctgaggcct cgccgggtca gtttgaaatc aacctttacc
    52921 atactgataa cgtgctggaa gcctgcgatg atgcgctggc actaaaacgc ctggtgcgtc
    52981 tgatggcaga aaagcataag atgcacgcca cttttatggc gaagccgtat gaagagcacg
    53041 cgggcagcgg aatgcatatc catatcagta tgcaaaataa tcgtggcgag aacgtgcttt
    53101 ctgacgcgga aggcgaagat tcgccgctgc tgaaaaagat gctcgccggg atgattgacc
    53161 tgatgccgtc gtcgatggcg ttgctggcac caaacgtgaa ctcgtatcgc cgcttccagc
    53221 cgggaatgta tgtgccgacg caggcgtcgt ggggccataa caaccgcacc gtcgccctgc
    53281 gtattccgtg cggcgaccgt cataatcacc gcgtggaata tcgcgtggcg ggtgccgatg
    53341 ccaacccata tctggtgatg gcagcgattt ttgccggtat tttgcatggc cttgataacg
    53401 agctgccgtt gcaggaagaa gtcgaaggca acgggctgga acaggaaggc ttaccctttc
    53461 cgattcgcca gagcgatgcc ctgggtgagt ttatcgagaa tgatcacctg cgccgctatt
    53521 taggcgaacg cttctgccat gtctatcacg cctgtaaaaa tgatgagctg ttgcagtttg
    53581 agcgcctcat cacagaaaca gaaattgagt ggatgttgaa aaacgcgtaa taccccttgc
    53641 ggggttgttt catcggcctg agcggagcca ggcctgacat ttgctggtaa tccagacatt
    53701 tcccggatgt cgcgttatgg agcgcgggcg gcaacgggct tccgttaacg attaagactg
    53761 tgacgaggaa atgagatgat gccgatgatt aaatccccgc atggcgaggg aggctgcgta
    53821 tgcgcccctc ctgctactga ctggactccg ccccctttgc ttcccttgct aaacaggttt
    53881 gatttcaggt cgacacgacc gcaaacctta ttacgcaggg gaggcagcaa ttatggctat
    53941 taattcacca ctgaatattg ctgcgcaacc cggcaaaacc cgtctgcgaa aatcactgaa
    54001 attgtggcag gtggtgatga tgggtctggc ctatctcacg ccgatgaccg tatttgatac
    54061 ttttggtatt gtctccggca ttagcgacgg tcacgttccg gcgtcctatt tgctggcgct
    54121 ggcgggtgtg ctgtttaccg ctatcagcta cggcaaactg gttcgccagt ttccggaggc
    54181 cggttcggcc tatacctacg cgcaaaagtc gattaacccg cacgtcggat ttatggtcgg
    54241 ctggtcatcg ctgctggatt atctcttttt gccgatgatt aacgtcttgt tggcgaaaat
    54301 ctatctctcc gccctcttcc cggaagtgcc gccgtgggtg tgggtggtaa ccttcgtcgc
    54361 cattttaacc gccgcgaatc tgaagagcgt caacctggtc gctaacttca ataccctgtt
    54421 tgtactggtg caaatctcca tcatggtggt gtttatcttc ctggtagttc agggactgca
    54481 taaaggagaa ggcgttggca ccgtctggtc acttcagccg tttatcagcg agaacgcgca
    54541 cctgatcccg attattaccg gggcgacgat tgtctgtttc tccttcctcg gtttcgatgc
    54601 ggtgaccacg ctttcggaag agacgccgga cgccgcacgc gtgatcccga aagccatctt
    54661 cctgacggcg gtctacggtg gcgttatctt tatcgcggcg tcgttcttta tgcagctgtt
    54721 ctttcccgat atcagccgct ttaaagatcc ggatgccgca ctgcctgaaa ttgcgctgta
    54781 cgtcggcggc aagctcttcc agtcgatttt cctctgcacc acgtttgtga acacgttagc
    54841 ctctggcctg gcttcacatg ccagcgtgtc gcgtctgctg tatgtgatgg ggcgtgacaa
    54901 tgtgtttccg gagcgcgtgt ttggctatgt gcacccaaaa tggcggactc cggcactcaa
    54961 cgtcattatg gtcgggattg tcgcgttgtc ggcgctgttc ttcgatttag ttaccgccac
    55021 agcgttaatt aacttcggtg ctctggtggc gtttaccttc gtgaatctgt cggtgtttaa
    55081 tcatttctgg cggcgtaaag gaatgaataa aagctggaag gatcacttcc actatttgct
    55141 gatgccgctg gttggcgcgc tgacggtggg tgtgctgtgg gttaacctcg agtcaacgtc
    55201 actgacactc ggtctggtat gggcttcgtt aggcggcgca tatttatggt atctgatccg
    55261 ccgctatcgc aaagtgccgt tgtatgatgg tgacagaacg ccggtgagtg aaacgtaata
    55321 gtcggatgcg cggcgcatta tcctgcctga gggtaacgcg ccgcgccatt aactataaaa
    55381 agatgtatga cccggatcac gagtgctatc atccgccctt atgcttagcg cattaacccc
    55441 aggggcaaat aatgaaccac gatatcccgc taaaatattt tgatattgcc gatgagtacg
    55501 cgaccgaatg cgcagagcct gtcgcagagg ctgaacgcac accgctggcc cactacttcc
    55561 agttactgct cacccgtttg atgaacaatg aagagatcag cgaagaagcc caacatgaaa
    55621 tggctgctga agcggggatt aatcccgtac gcattgatga gatcgcagag tttttgaatc
    55681 aatggggtaa tgagtaatga tttaattgca gacgatcggt cagtatcgtt gtccggataa
    55741 agcgttcacg ccgcatctgg ctaacaatga cacgcactgc agcgtgcttt aatgggtttc
    55801 tcacgatcgt accgctctgc tcctcgctaa gttgttctgt gagttatagt tatagagaaa
    55861 tcacgggtac gaatgttgaa cagatggcga cgtcacctgt tcaacgacca accacacggc
    55921 cctcattacg acccctgaac acaaacggta atggcaaatg tcattaaata ttagtaagat
    55981 agcctcttta cataaaatcc cttaatatta tgcgccaggt attatcgtct cttttggtga
    56041 ttgctggact tgtgagtggt caggcaatcg ccgcgcctga atctcccccg catgctgata
    56101 tccgcgacag cggttttgtc tattgcgtca gcgggcaagt caacaccttt aacccatcca
    56161 aagcgagcag tgggttaatt gtcgataccc ttgccgccca gttttatgat cgactgctgg
    56221 atgtcgatcc ctatacctat cgcctgatgc cggaacttgc cgaaagctgg gaagtactcg
    56281 acaacggcgc gacctatcgc ttccacctgc gtcgcgatgt tccgtttcaa aaaaccgact
    56341 ggtttactcc cactcgtaaa atgaatgccg acgatgtggt gtttaccttc cagcgaattt
    56401 ttgaccgcaa caacccgtgg cataacgtca acggcagcaa cttcccctac ttcgacagcc
    56461 tgcaatttgc cgataacgtg aaaagcgtcc gcaaactgga taatcatacc gttgagttcc
    56521 gactcgccca gccggatgct tcttttttgt ggcacctcgc aacccattat gcttcggtca
    56581 tgtcggcaga atatgcccgg aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc
    56641 aaccggtcgg caccggtccg tatcagttgt cggaataccg cgccgggcaa tttattcgcc
    56701 tacaacgtca tgatgacttc tggcgcggta aaccgttaat gccgcaggta gtggtggatt
    56761 taggctccgg cggcaccgga cgtctgtcga aactcctgac cggggaatgc gacgttctgg
    56821 cctggcctgc tgccagccag ctatccattt tgcgtgacga cccgcgcttg cgtttaacgc
    56881 tgcgtcctgg gatgaacgtc gcctatctgg catttaacac cgccaaaccg ccgctaaata
    56941 atcccgctgt ccgccatgcg ctggcactgg cgattaataa ccagcgcctg atgcaatcca
    57001 tctattatgg tacggctgaa acggcggcct ctattttacc gcgcgcctcg tgggcctatg
    57061 acaacgaggc taaaattact gaatacaatc cggcgaaatc gcgcgaacag ttgaagtcgt
    57121 tggggctgga aaatttaacg ctgaaactgt gggtgcccac acgttcgcag gcgtggaacc
    57181 ccagtccact gaaaactgcc gaactgattc aggcggatat ggcgcaggtt ggcgtaaaag
    57241 tggtgattgt gccggtagaa ggtcgctttc aggaggcgcg gttgatggat atgagccatg
    57301 atctgacgtt atccggttgg gcgacggaca gtaacgaccc ggacagtttc ttccgtccgt
    57361 tactgagctg cgcggcaatt cattcgcaga ccaacctcgc ccactggtgc gatccgaaat
    57421 tcgacagcgt attgcgtaag gcgctctctt cgcagcagct ggcggcgcgt attgaagcct
    57481 atgacgaagc gcagagtatt ctggcgcagg aattgcccat tttgccgctg gcgtcgtcat
    57541 tgcgtttgca ggcctatcgg tacgatatca aaggtctggt acttagcccg tttggtaacg
    57601 cctcctttgc tggggtgtat cgcgagaaac aggatgaggt gaaaaaacca tgattatctt
    57661 caccttacgc cgcattttgt tattgattgt caccctgttc ctgctgacct tcgttggctt
    57721 cagcttgagc tatttcaccc ctcatgcgcc gttacaaggc gcgtcactgt ggaatgcctg
    57781 ggtgttctgg tttaacggcc tgatccactg ggattttggt gtttccagca ttaacggtca
    57841 accgattgcc gaacagttaa aagaggtctt cccggcgacg atggaactgt gcatcctcgc
    57901 cttcggtttt gcgctgattg ttggcatccc cgtggggatg attgccggga ttacgcgcca
    57961 taagtggcag gataacttga tcaacgccat cgccttactg ggcttttcaa tcccggtgtt
    58021 ctggctggcg cttctgttga cgctgttttg ttcactcacg ctaggttggt tgcccgtttc
    58081 agggcgtttc gatctgctct acgaagtgaa accgattacc ggttttgcgt tgattgatgc
    58141 ctggctttcg gactcaccat ggcgggatga aatgatcatg agcgcaatcc gccatatgat
    58201 attgcccgtg atcaccctgt cggttgcgcc aacaactgaa gtgatccgcc tgatgcgtat
    58261 cagcactatc gaagtgtatg accagaacta tgtaaaagcg gcggcgaccc gcggtttgtc
    58321 gcgctttact attttgcgtc gccacgttct gcataacgcg ttaccgccgg ttattcctcg
    58381 cctgggctta cagttttcca ccatgctgac gctggcgatg atcaccgaaa tggtttttag
    58441 ctggccgggt ttagggcgct ggttaattaa cgccatccgc cagcaggatt acgcagccat
    58501 ttccgccgga gtgatggtgt gtggctcact ggtcattatt gttaacgtga tttctgatat
    58561 tttgggtgcc atggctaacc ctctgaaaca taaggaatgg tatgccttac gatagcgtat
    58621 acagcgaaaa gcgcccgccg ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg
    58681 cctctgcgat ggtcggcctg tacggctgcg cggggctggc tgtactgtgt atttttggcg
    58741 gctggtttgc gccttacggc atcgaccagc aatttctcgg ttatcaatta ttgccgccgt
    58801 catggtcgcg ctatggcgaa gtttctttct tcctggggac tgacgacctc ggacgcgatg
    58861 ttttaagccg tttactgagc ggagctgcgc caaccgtggg tggcgcattt gtggtgacgc
    58921 ttgccgcgac gatctgtggc ctggtactgg gcaccttcgc cggggcgaca cacggcctcc
    58981 gctcagcggt gcttaaccat attctggata ccttactggc gatcccttcg ctgctgttgg
    59041 caattatcgt tgtagcgttt gccggaccga gtttgtccca cgctatgttt gctgtctggc
    59101 tggcattgct gccgcgtatg gtacgttcga tttacagcat ggtgcatgac gaactggaaa
    59161 aagagtacgt tatcgccgcc cgtctggatg gcgcatcaac gctgaatatt ctctggtttg
    59221 ctgtgatgcc aaacatcacc gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg
    59281 caattctcga tatcgccgcg ctgggctttc tcgatctcgg cgcacaactc ccctcgcctg
    59341 aatggggagc gatgctcggt gatgcgctgg aactgattta tgtcgccccg tggactgtca
    59401 tgctgccagg tgcggcaatt atgattagcg tgttgttggt taacctcctt ggcgatggcg
    59461 ttcgtcgtgc gattattgcg ggggtggaat aatgccatta ctcgatattc gtaacctgac
    59521 cattgaattt aaaaccggtg atgagtgggt taaagccgtc gaccgggtaa gtatgacctt
    59581 aaccgaaggt gaaatccgcg gtcttgttgg tgaatccggt tccggcaaga gtttgattgc
    59641 gaaagcgatt tgtggggtga ataaagataa ctggcgtgtt actgctgacc gtatgcgttt
    59701 tgatgatatc gatttgctgc gtctctccgc acgcgaacgg cgcaaactgg ttggccataa
    59761 cgtgtcgatg attttccagg aaccgcagtc gtgtcttgac ccttcagaac gtgtgggccg
    59821 ccagttgatg caaaacatcc cagcctggac ctataaaggc cgttggtggc agcgttttgg
    59881 ctggcgcaaa cgccgtgcca ttgaactgct gcaccgcgtg gggattaaag atcacaaaga
    59941 tgcgatgcgc agttttccct atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc
    60001 cattgcactg gcgaatcaac cgcgtctgct gattgctgac gaaccgacca actcaatgga
    60061 gccaacaacc caggcgcaaa tctttcgcct gctgacgcgt ctcaaccaaa acagcaatac
    60121 cactattttg cttatcagcc atgacttaca aatgcttagc caatgggcgg ataaaattaa
    60181 cgtgctttac tgcggtcaaa cagtggaaac cgcgccgagt aaggagttgg tgacgatgcc
    60241 acatcatcct tatacccagg cgctgatccg cgcgatacca gacttcggca gcgcgatgcc
    60301 gcataaaagt cgcctcaata cgctgcccgg cgctatccca ctgctggaac agttaccgat
    60361 tggttgtcgt ctggggccac gttgcccgta tgcacaacga gaatgtattg tgacgccacg
    60421 tttgacgggg gcgaaaaatc atctctatgc ctgtcatttc ccgctgaaca tggagaaaga
    60481 gtgagatgat cgaaacgctg cttgaagtgc gtaatttaag taaaacgttc cgctaccgga
    60541 ccggctggtt tcgtcgtcag accgtagaag cggtaaaacc cttgagcttt acgctacgtg
    60601 aaggccagac actggcgatt attggcgaga atggttcggg taaatccacg ctggcaaaaa
    60661 tgctggcggg aatgatagag cctaccagcg gcgagttatt gattgacgat catccactgc
    60721 attttggcga ttattccttc cgcagtcagc gtattcgcat gatttttcag gatccttcga
    60781 cctcgttgaa tccccgtcaa cgtatctcgc aaattctgga ttttccactg cgcctgaaca
    60841 ccgacctgga accggaacag cgtcgcaaac agattattga aacgatgcgt atggttgggc
    60901 tgctaccgga tcacgtcagt tactatccgc atatgttggc acccggacaa aaacagcgtc
    60961 tgggtctggc gcgcgcgctg atattgcgcc caaaagtcat tattgccgat gaagccctcg
    61021 cttcactgga tatgtcgatg cgttcgcagt tgattaatct gatgctggaa ttacaggaaa
    61081 aacagggcat ttcgtatatt tatgttaccc agcatatcgg aatgatgaag cacattagcg
    61141 accaggtgct ggtgatgcat cagggcgagg ttgtcgagcg aggcagcacc gcggatgtgc
    61201 tggcatcgcc gctgcatgaa ctcaccaaac ggctgattgc cggtcatttt ggcgaggcat
    61261 tgacggcgga tgcgtggcgt aaagatcgct aattttctga atcctccttc cagattattc
    61321 acctcattct gacaaaagat taaatcttgt cagaatgagg tgatagataa aattaaatca
    61381 aatgcgcgtg atttaagacg caatctcact ttgcaggaac gaaagctctg gcgatatctt
    61441 cgcagccgac gttttagtga tttcaaattt cgccgtcaac atccagtggg gagctacatt
    61501 ctcgattttg cttgctgctc ggcgcgtgta gtcgttgagc tggatggtgg gcagcatgat
    61561 ttagcagttg cctatgattc caggcgcact agctggcttg agtcgcaggg ctggaccgtg
    61621 ctgcgtttct ggaataacga gattgattgt aatgaggaga cggtgctgga gaatattctg
    61681 caggaactga accgccggtc accctctccc tgaaagagcg agggggcaga ccgagccgaa
    61741 tagctgttgt ggtgaaaaca tggagacggt gctggagaat attcggcaag gtctgaaccg
    61801 tcccagccat cgccatgaaa gggttagggg ctgtatgagc ctgtttgttg ctggggtaac
    61861 aatatttgca caatacggtc ccctcgcccc tctggggaga ggattagggt gaggggaaaa
    61921 gcgccccccc tgccgcagcc tgctccggtc ggacctggca actatagcta ctcacagcca
    61981 ggttgattat aataaccgtt tatctgttcg tactgtttac taaaacgacg aatcgcctga
    62041 ttttcaggca caacaagcat caacaataag gattaaagct atgggttttc tttccggtaa
    62101 gcgcattctg gtaaccggtg ttgccagcaa actatccatc gcctacggta tcgctcaggc
    62161 gatgcaccgc gaaggagctg aactggcatt cacctaccag aacgacaaac tgaaaggccg
    62221 cgtagaagaa tttgccgctc aattgggttc tgacatcgtt ctgcagtgcg atgttgcaga
    62281 agatgccagc atcgacacca tgttcgctga actggggaaa gtttggccga aatttgacgg
    62341 tttcgtacac tctattggtt ttgcacctgg cgatcagctg gatggtgact atgttaacgc
    62401 cgttacccgt gaaggcttca aaattgccca cgacatcagc tcctacagct tcgttgcaat
    62461 ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc ctgctgaccc tttcctacct
    62521 tggcgctgag cgcgctatcc cgaactacaa cgttatgggt ctggcaaaag cgtctctgga
    62581 agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa ggtgtgcgtg ttaacgccat
    62641 ctctgctggt ccgatccgta ctctggcggc ctccggtatc aaagacttcc gcaaaatgct
    62701 ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt actattgaag atgtgggtaa
    62761 ctctgcggca ttcctgtgct ccgatctctc tgccggtatc tccggtgaag tggtccacgt
    62821 tgacggcggt ttcagcattg ctgcaatgaa cgaactcgaa ctgaaataat cgttctgttg
    62881 gtaaagatgg gcggcgttct gccgcccgtt atctctgtta tacctttctg atatttgtta
    62941 tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt cctttacgac
    63001 caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta tcatgaaacg
    63061 caatccagta ctacggagta tgacgttctg cctctggtcc cggaagccgc aaaggtcagc
    63121 gatccctttc tactcgacgt gatccttgaa aaagaaacgc tggccccctt cctttcatgg
    63181 ctggaccctg cgcgtgttct tgcagtggat ttgttccctg accagcttac cgtgacccgt
    63241 tcacagacct tcaccgctta tgaacgcttg tcgacggccc tgacggttgc tcaggtttgc
    63301 ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc cgggcctgat
    63361 tccaccaggg aaagtaatca tcggttggca caaatcacgc aatatgcccg ccaactggct
    63421 agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt cggtcttact
    63481 gcctgggact gtgtgatcat tagccaaatc attggtttta ttggctttca ggcgcggaca
    63541 attgcgacat ttcaggctta tctcgggcat ccggtacgct ggttacccgg gctggagata
    63601 caaaactacg ccgacgcgtc actgtttgct gatgaatcat tacgctggcg aagcagctat
    63661 gaggtggaaa aactacctga agagcacaca aaaagttcaa ctgcagaact ttgccaactg
    63721 gccgaaatac tctctctcca ccctatttca ctttcccttc tcgaaaagtt gttaaacagc
    63781 acacggggca atacacagcc ggataatcag cttgcggcgt tgttatgcgc gcgtataaat
    63841 ggcagtcctg cttgttttgc cacctgtatg gattcatcaa atgaatataa aaaaatcagc
    63901 acccttatgc gcaagggcga aaatgaaatt aaccaatggg ctgaccgtca ttctgttgag
    63961 cgcgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt tagcgccgcc
    64021 cagttcagcc ctttactcga acacgaaaaa tcatcaacgc agattattaa tctgctggta
    64081 tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga gacatattaa
    64141 ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc caccaaaata
    64201 gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac tgcattccca
    64261 gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct tcctggaagt
    64321 cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca tgcatggcga
    64381 ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc cagaagaact
    64441 ggttgaaccg ttcctgactc gtttcgtggg taaggttcag ggcaaaaatg accgtctggc
    64501 catcgttcct gatcatccac tcttaaaaga cgccattcct tgccgcgcag cccgtggcct
    64561 gaaccacgag tttaaagaag gcgactgggc ggttgccgaa atgcgccgtc atccgctgaa
    64621 aggcgatcgt tctttctatg cagaactgac acaatacatc acttttggtg acgatcactt
    64681 tgtaccgtgg tgggttaccc ttgcacgcca taatctggaa aaagaagcac cagacggcgt
    64741 cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgaccgcgc tggattttgt
    64801 caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcta aggcgttgcc
    64861 ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga ttgctgaagg
    64921 cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc ctggcttcaa
    64981 catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg ccaatgaagt
    65041 ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcaccattg aagataatat
    65101 cgaattcttt gccgccacca tcgaatccaa agcaaagctg gtgtatgacc aggtttctga
    65161 ctggctggaa aataccggtg actggcagcc tgaaagtgaa gcaattgccg aacaagtccg
    65221 tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg cactggtgtt
    65281 taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc tggatatcgt
    65341 cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg ccgctaacat
    65401 ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg tgcatatggg
    65461 ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg gtctgcatgt
    65521 cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg aactggacgc
    65581 gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg ctgaaattag
    65641 cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct ggacttcgcc
    65701 gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta tcaaaggcga
    65761 aactgcgacg cgcccacagg atgaaatcac tgtccaaatg gccgagcgtc gccgtctcaa
    65821 ccggatggca gaacgtgatg ttggtgactg gttatacgca cgcttcctga aagacaaagc
    65881 cgggaccgac acccgtttcg cagcggaaat tgtcgatatc agccgtggcg gcatgcgtgt
    65941 tcgtttggtt gataacggcg ctatcgcctt tattccggca cctttcttac acgctgtgcg
    66001 cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa attaaaggtg aaacggttta
    66061 caaagtgact gacgttattg acgtcactat tgccgaagtc cgcatggaaa cccgcagcat
    66121 tattgcgcgc ccggtcgcgt aatctccttt cacggcccat tcctcatgga tgggccgttt
    66181 atttccccgc tctaccttcg ttatatccag gcagattttt taactgactt tcgtttgaaa
    66241 actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaaacaa tgcgtttagc
    66301 cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac tccgctgtta
    66361 accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac aactttctcg
    66421 gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgttttgcgc ttttctacca
    66481 ccgaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct gctcgcatca
    66541 gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat gaaagcgatc
    66601 tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc aacgcgtctg
    66661 catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc tttgctgagc
    66721 aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg aatatccaac
    66781 gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc attgggcaaa
    66841 gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc aataaccgtg
    66901 tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca tgtaaaggcc
    66961 agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa aacgagattt
    67021 ttttaatctg ttccggcacc gacattaccg aagagcgccg cgctcaggag cgactgcgta
    67081 ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg caggatttaa
    67141 tcgatcacgc tattaatcat gcagataaca ataaagttgg ggttgtgtat cttgatttgg
    67201 ataatttcaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag ttattacgcg
    67261 acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg cgtccaggtg
    67321 gggatgagtt tctggtactg gcatccaaca cctcacaaag cgcgctggaa gcaatggcat
    67381 cacgaatttt gacccgctta cggctcccct ttcgcattgg tttaattgaa gtttatacca
    67441 gctgttcagt aggtattgca ctctctcccg aacatggttc agacagcacg gctattattc
    67501 gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa ttttgcgttt
    67561 ttaccccaga aatgaatcaa cgggtatttg aatatctctg gctggatacc aacttgcgta
    67621 aagcactgga aaacgatcag ttggttattc actatcaacc gaaaatcacc tggcgtggcg
    67681 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    67741 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    67801 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    67861 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    67921 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    67981 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    68041 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    68101 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    68161 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    68221 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    68281 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    68341 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    68401 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    68461 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    68521 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    68581 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    68641 ctgaatctac gccagacatc ccgaaggcac cgtgggagat ttaacaatcg tgccttcagg
    68701 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt attaagtatt ataacgttat
    68761 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    68821 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    68881 ggatctcaac acactcgaaa aactgagtta cctccgccgt gcacatggct ttgctgtttc
    68941 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    69001 cgccgagttt gccgcgtcgc tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    69061 cagcaatgcc ctactcgccc ggactctggg cgagcagaag aaaaatgtca ctatcatcac
    69121 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtta ttttgctcgg
    69181 tggcgtgtat cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    69241 acaggtgcat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    69301 taccggtcgc gatatgatgc gtaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    69361 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    69421 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    69481 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    69541 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    69601 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    69661 gtcattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    69721 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    69781 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    69841 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    69901 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    69961 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    70021 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    70081 ataatacgca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    70141 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    70201 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    70261 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgacaccg gtaatcaggc
    70321 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccaca ccgtcgcctt
    70381 tagggcgtac aggggccgct ttgggctcat caatacgtcc ggtttccgtt gagtagacca
    70441 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    70501 cgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    70561 agccgacaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    70621 cggacgaatg cccggcgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    70681 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgccgccag
    70741 acgttctgca taatctgcag gtgacagtgt catgccaaga tcgaccaggt cgctggcttc
    70801 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    70861 tgcctcacgc gctgcggtca tcatacgcgc cccaccagag gcatgaacat tcaccatcca
    70921 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    70981 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    71041 aaacaatgta aacatctctt tgccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    71101 gacaaaggcc agcgcgtcat cacgattatg ataatcaagg gcaacaacca caggagaatt
    71161 cgtaacagcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    71221 ggcaccggaa gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtgg aaataaaaaa
    71281 ttaacagttg cgatttctcc cctggcaggc tttttctgcg ccgtcgttgc cggagcacaa
    71341 tggaacatta ataattagta tgttgtaact aaagtcgggt tttttttaaa aattacaggc
    71401 catcaagacc gcgaatcggt ttaatggttg accaggcccg acaagacgga caatgccagt
    71461 agagggtgta tgcggtaaaa ccacatttct ggcaacgata acgaggctta ctacgtacct
    71521 tctcgccaac catgtcacgc agcaccatca ggctctcttt ggcacgccct tcttccgctt
    71581 catttaagtg gtaatccatt aacttatgga acacacgcat ggtcggatga cgctgaagct
    71641 ggcgcgtaat atagacctgt gcggcctcac taccgtcgcg cgcttcgatg atatcagcaa
    71701 gcatcaattc agcatcggca ccggtgttct cttccaccgc acgctgcagg aattctgccc
    71761 attcggcagt tttacccaac tgctgatagc aggtttgcaa catttccagc gtttcgctga
    71821 ccagttctct gtcctgggat atcacgcgtt gcagactttc gacggctttg gcgtattctc
    71881 ctttcgccat aaacacgcgt cccatcatta tggatacgcg ggcgctgttt ttatctgccg
    71941 ccgccccttt ttttagcaag gtcatggcac gatcgagatc gtcgctggcc atatgctgca
    72001 gggctaactc acagtagaaa tgggcaattt cgacgcgctg tttatcttta cccagcttca
    72061 ccaggcgttc ggcaacatca attgctttct gccactcact ggtagcctgg tagatttgta
    72121 gcaactgttg cagcgcgcca atgcggaagt cagtttcatc ggtcagctga ttgaacatgt
    72181 cttccgcgcg gtcatataac ccggcggcca tgtaatcacg ccccagttgt tgaatcgcca
    72241 acagacgctg ttcataggtc agcgaggcgc tttccattag ggtctgatgg atgcgaatag
    72301 cgcgatcaac ttcgccacgc gaacggaaca ggtttccgag cgtaaggtgg gcttcaacgg
    72361 tgcctgtatc ctctttaagc atatcgagaa acaggtctac cgctttatcc tgttgattac
    72421 taagcaggaa gttaaccccc gctacgtaat cacgcgacaa gcggttggct tcatcttgct
    72481 tgttttgttg cgcacttctg cggcccatat accagccata ggcagcggct acaggcaaaa
    72541 gcagaaacaa caactccagc atagaaagtt attccttcgc cgctgacgag tgcggcacta
    72601 cagccacgtc agtcgcgggt gaaagctggt tttccagtcg ctttatttta cgttcagcgc
    72661 gcgccaggga aacacgaact cgcagccaga acaggccaca aatcaaccaa ccgatagcaa
    72721 accccgcagc aaacaatacc gccagcaatg tggaaatacg gtactcccct tgcgctaaca
    72781 gataattaaa cgtcacctgt tgatcgttct gcgcacccaa cgtgaccgaa atcacgaaga
    72841 tcgctaacac cagtaagaaa atgagtaaat atttcacatt acttcccgtt atgtggttag
    72901 agcgaataaa ttatggtcaa cttaccgcaa tagcccttta aagtaccatt ttcgcgccgt
    72961 ctgcgaaatg gcatccacac gcattgttga tgatttaagg gctaaattcg gaaaatcaac
    73021 cagcgttaac tttcttgttc tcgttgcgct atttcgcgat tttcttccgc aggtggtgtt
    73081 aatggcccac aaattcgttg cgcaagccac gttgccaccg ccaccagcgc ccacgaaatc
    73141 aacgtagcta ctaccagatc gcgtggccaa tgcatcccga gcagcaggcg gcttcccatg
    73201 actcccgttg cccagaccag caagatagca atggttaacg ttcgccgacg cggccacagc
    73261 aaaccaacgg ccagcagtgc ccaactggca gcaaacatcg tgtgaccgga aggaaaggca
    73321 aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg ggatattttt ctcttcagcc
    73381 aactgttctt tcactagatt tccgcgttct gctcgcttta aagtgtagaa ctcatcaacc
    73441 ggaatatgat gtgttttttc cagccagata acaaaaggtc gtggttcctg gactttgtct
    73501 ttgatccagg atttaacgcc ttgtcccaca aggattgcgg ccgccagaat ggcaaataat
    73561 acaaaggcag ccttaatgcg aaaacgcaga caccagagaa accagccgaa taaaatcaaa
    73621 tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa cccaaaaagc cgcttttagt
    73681 agccaacttt gttctccagg ttgccaacgc cagccagaaa tccatacggc tactggcatg
    73741 acaagcaata gtgcagctcc cactgcggta cgtctggcaa tcgaacgcat ggcctctcct
    73801 ttttgataag tcccacaatc ataactgaaa acgccagttc caggaaaaat tgacagattt
    73861 gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga accccttctc gttatggcaa
    73921 aataagccaa tacagaacca gcattatctg gagaatttca tgcagcttaa acgtgtggca
    73981 gaagccaaac tgccaacccc atggggcgat ttcctgatgg tgggatttga agaactggca
    74041 accggacacg atcatgtcgc gctagtctat ggcgatattt ccgggcatac cccggtactt
    74101 gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt tcagcttgcg ctgcgattgt
    74161 ggcttccagc tcgaagcggc attgacgcaa attgccgagg aaggccgtgg tattttgctg
    74221 tatcaccgtc aggaaggtcg taacattggt ctgctgaata aaatccgcgc ttacgcactg
    74281 caggatcaag gttacgatac cgtagaggct aaccaccagt taggcttcgc cgctgatgag
    74341 cgcgacttca ctctttgcgc tgatatgttc aaactccttg gcgtcaatga agtccgcttg
    74401 ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag cagggattaa tattgttgaa
    74461 cgcgtaccat tgattgtagg tcgtaacccc aataacgaac attatctcga taccaaagcc
    74521 gagaaaatgg gccatttgct gaacaaataa ccctcttgca ttgtgtaatt catttgcttg
    74581 ccggaagcaa aataaccggc aagcaaatag ttgttacttc aacatattac gaatgacata
    74641 atgcaaaatg ccgtcgttct ggtagtaggt caactccgtc gcggtgtcga tacgacaacg
    74701 gcagggtacg acttcctggc taccatccgc gcgcgtaagc gtcaccggaa ccgtcgcgcc
    74761 gggttgtagg ttttgcagat cgccaatatc aatcttctct tccccggtta gccctaacgt
    74821 tttacgcgtt acgccttgcg gaaattccag cggcaggatg cccatgccaa ttaaattcga
    74881 acggtgaatt cgttcaaacg attcggcaat caccacacga ataccaagca gacgcggacc
    74941 ttttgccgcc cagtcacgac tggagcctga tccatactct ttcccggcaa tcaccgccag
    75001 cggcgtttgc tcctgcttat agcgcatcgc agcatcataa atagagacta cgtcgctgtc
    75061 aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc atttcattac ggatgcgaat
    75121 attggcgaag gtgccacgca tcatcacttc atggttacca cgccgcgaac cgtaggagtt
    75181 aaagtctttt cgctcaacac cccgaccttg tagatatcga cccgctgggc tgtcgggctt
    75241 aatactgccc gccggagaga tatggtcagt ggtgactgaa tcccccagca ttgcgaggat
    75301 ccgcgcaccg tgaatatctt ccactggtgc tggtgttgcc tgcatttcat caaagaaagg
    75361 cgataagcga atataggttg agtcctcctg ccaaccgtag gtatcggatc gtgtgacgtt
    75421 aattcccttc cactctgctg tgccttcaaa aacttctgcg tactctttgc ggaacatttc
    75481 tgtggagact tgttctaccg cacgggcaat ttcttgtgcc gatggccaga tatctttcag
    75541 ataaaccgga tcgcctttgc gatcatggcc gataggctca gaagccaggt tgatattcat
    75601 atttcccgcc agcgcatagg caaccaccag cggcggcgag gccagccagt tagttttaac
    75661 cagcggatgg atacggcctt caaagttacg gttgccggac agcaccgcac cgacggttaa
    75721 atcgcctttt ttgattgccg tttcgatagg atcgggcagc ggcccagagt taccaataca
    75781 ggtggtacaa ccgtatccca caaggttaaa ccccagttcg tcgagatacg gtgtcagttt
    75841 cgcttttgcc agataatcag aaacgacttt cgaacccggt gccagcgacg ctttgaccca
    75901 tggttgccgc ttgaggccca gagttacggc ttttttcgcc agcaagcctg cggccatcag
    75961 cacacttggg ttagaggtgt tggtgcacga ggttatcgca gcaatgacca cagcgccatc
    76021 aggtaactga tactgatgtc cgttcataac ataatcgacc ggctggcgat ctttatgcgt
    76081 ggcattcact tccagttcgt tactggcggc aaatgctttt ggtacatcgg gcagtgcaac
    76141 gcgatcctgt gggcgtttag gccctgccag gctcgcttca acgtcattca tatccagttc
    76201 taacgtactg gtaaaaattg gttcatcgcc cgggttacgc cacatgccct gcgctttggc
    76261 atatttttcg accaactcga cctgatcttc gctgcgcccg cttaaacgca tgtaatcgag
    76321 ggttacagca tcgattggga agaagccaca ggtggcacca tattctggcg acatattggc
    76381 aatggtggcg cgatccgcca acggtagtga atccagacca tcaccataaa attcgacgaa
    76441 tttccccacc acgccatgtt tgcgcagcat ttgggtaaca gtgagaacca ggtctgtggc
    76501 ggtaatacct tcacgtaatt ttccggtaag tttgaagccc actacatccg ggataagcat
    76561 ggaaaccggc tggcctaaca ttgcggcttc tgcttcgatc ccaccaacgc cccaccccag
    76621 cacgccaagg ccgttgatca tggtggtgtg cgagtcagta ccaacgagtg tatccggata
    76681 agcaatccat tcaccgtcct gcaattcact ccacactgct ttgccgagat attcgaggtt
    76741 aacctgatgg caaatgcctg tgcctggcgg cacgacgcta aaccgactga acgcttgctt
    76801 tccccatttc aggaacacat aacgttcgtg gttgcgctcc atttccaggc gtacgttttc
    76861 ttcaaatgcc tcatcatcac caaaacgatc gacggtcacc gagtggtcaa tgaccaggtc
    76921 gaccggtgag agcgggttaa cctttgcagt atcgccgccg aggcgtttaa ccgcttcgcg
    76981 cattgccgcc agatcaacaa cggcaggtac gccggtaaag tcctgcatca gcacccttgc
    77041 cgggcggtag gcaatttcac ggtcagcatg ggcatttttc agccatcctg ccagcgcgtg
    77101 gatatcctct tcggtaaccg agttaccatc ctgccagcgc agcaggtttt cgagcaaaac
    77161 tttgagtgac ttgggtagac gggtgatatc gcccagtgat ttagcagcaa gcggcaggct
    77221 gtagtagtgg taagttttat ctttggcctg caacgtgtcc ttactggctt ctcgtagggt
    77281 tgacgacata gctcctcctt aatgacaggg ttgcgaagcc ctgattctct tcagggtgaa
    77341 tattaaagat aacacaaaca tcgcgtaacg atttgataac aacccaaatt gataaaagag
    77401 gagaaacctt gtggcaaagc aaatgacaac cccgccgcag cggggtcaag gatcagtgag
    77461 taatgatcca gaggagttcg gcccagaaga gcatcgacag agaaaacgcg cccatccagg
    77521 accaatattt catatttgtg ttgatcatag gctacaccct taacggttat tattttgtgt
    77581 tgagataact caagctgtta ttgctgcggt gtattaaaag ccctttaatt cattaaaaat
    77641 gggtaataag aatgatgata attattgttt cggctgatca tcttcagcaa acagttctat
    77701 aaaggcttgc tgctgcgatg ttagttgcca gtcgtctgga ataacgacgg aacattcccg
    77761 tttcgtttct gtttcattgt cactatgctg gcataatggt gacgactcca gttgctcctg
    77821 accgaccata gttatcccca cactttccaa atcagcagtg cgacaaccac ccagaaaagt
    77881 gcagaaccaa gaaaaaccgc cagccaggct ttacgtttta attcaggatc gcggcggggt
    77941 tcctgatttc cagaaggcat tgctaacctc atataatcgc catcacttat cagcaagacg
    78001 ttgaacgatt ggtgtaatta atcatacgat gagacaaatc ctaaagaaac tttagtcaaa
    78061 aatccagcga atttacgaat cgtctccagt gaataattca atcttttgac ctgaaataga
    78121 tatttgaaaa ctggagtttg cgaggcgggt aattagacac tactttacag gtaatttacc
    78181 ctataaatat gagggataaa a
//
LOCUS       DBBGLQ010000015        76349 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.015, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000015 DBBGLQ010000000
VERSION     DBBGLQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.015"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            5..877
                     /gene="ygcG"
                     /locus_tag="ACIYSB_001305"
     CDS             5..877
                     /gene="ygcG"
                     /locus_tag="ACIYSB_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGC5428387.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(937..2235)
                     /gene="eno"
                     /locus_tag="ACIYSB_001306"
     CDS             complement(937..2235)
                     /gene="eno"
                     /locus_tag="ACIYSB_001306"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGC5428388.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2323..3960)
                     /gene="pyrG"
                     /locus_tag="ACIYSB_001307"
     CDS             complement(2323..3960)
                     /gene="pyrG"
                     /locus_tag="ACIYSB_001307"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGC5428389.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4188..4979)
                     /gene="mazG"
                     /locus_tag="ACIYSB_001308"
     CDS             complement(4188..4979)
                     /gene="mazG"
                     /locus_tag="ACIYSB_001308"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGC5428390.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVGQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5050..5385)
                     /gene="mazF"
                     /locus_tag="ACIYSB_001309"
     CDS             complement(5050..5385)
                     /gene="mazF"
                     /locus_tag="ACIYSB_001309"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGC5428391.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5385..5633)
                     /gene="mazE"
                     /locus_tag="ACIYSB_001310"
     CDS             complement(5385..5633)
                     /gene="mazE"
                     /locus_tag="ACIYSB_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGC5428392.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5711..7945)
                     /gene="relA"
                     /locus_tag="ACIYSB_001311"
     CDS             complement(5711..7945)
                     /gene="relA"
                     /locus_tag="ACIYSB_001311"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGC5428393.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7993..9294)
                     /gene="rlmD"
                     /locus_tag="ACIYSB_001312"
     CDS             complement(7993..9294)
                     /gene="rlmD"
                     /locus_tag="ACIYSB_001312"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGC5428394.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9351..12107
                     /gene="barA"
                     /locus_tag="ACIYSB_001313"
     CDS             9351..12107
                     /gene="barA"
                     /locus_tag="ACIYSB_001313"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGC5428395.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12339..13679)
                     /gene="gudD"
                     /locus_tag="ACIYSB_001314"
     CDS             complement(12339..13679)
                     /gene="gudD"
                     /locus_tag="ACIYSB_001314"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGC5428396.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13700..15039)
                     /gene="gudX"
                     /locus_tag="ACIYSB_001315"
                     /pseudo
     CDS             complement(13700..15039)
                     /gene="gudX"
                     /locus_tag="ACIYSB_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(15041..16393)
                     /gene="gudP"
                     /locus_tag="ACIYSB_001316"
     CDS             complement(15041..16393)
                     /gene="gudP"
                     /locus_tag="ACIYSB_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGC5428397.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNKKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16828..17277)
                     /gene="yqcA"
                     /locus_tag="ACIYSB_001317"
     CDS             complement(16828..17277)
                     /gene="yqcA"
                     /locus_tag="ACIYSB_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGC5428398.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17295..18077)
                     /gene="truC"
                     /locus_tag="ACIYSB_001318"
     CDS             complement(17295..18077)
                     /gene="truC"
                     /locus_tag="ACIYSB_001318"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGC5428399.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18077..18406)
                     /gene="yqcC"
                     /locus_tag="ACIYSB_001319"
     CDS             complement(18077..18406)
                     /gene="yqcC"
                     /locus_tag="ACIYSB_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGC5428400.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19028..19573)
                     /gene="syd"
                     /locus_tag="ACIYSB_001320"
     CDS             complement(19028..19573)
                     /gene="syd"
                     /locus_tag="ACIYSB_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGC5428401.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19641..20489
                     /gene="queF"
                     /locus_tag="ACIYSB_001321"
     CDS             19641..20489
                     /gene="queF"
                     /locus_tag="ACIYSB_001321"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGC5428402.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSLQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20601..21965
                     /gene="ppnN"
                     /locus_tag="ACIYSB_001322"
     CDS             20601..21965
                     /gene="ppnN"
                     /locus_tag="ACIYSB_001322"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGC5428403.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22522..23811
                     /gene="sdaC"
                     /locus_tag="ACIYSB_001323"
     CDS             22522..23811
                     /gene="sdaC"
                     /locus_tag="ACIYSB_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGC5428404.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23869..25236
                     /gene="sdaB"
                     /locus_tag="ACIYSB_001324"
     CDS             23869..25236
                     /gene="sdaB"
                     /locus_tag="ACIYSB_001324"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGC5428405.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPSFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25348..26103
                     /gene="xni"
                     /locus_tag="ACIYSB_001325"
     CDS             25348..26103
                     /gene="xni"
                     /locus_tag="ACIYSB_001325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGC5428406.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26158..27306)
                     /gene="fucO"
                     /locus_tag="ACIYSB_001326"
     CDS             complement(26158..27306)
                     /gene="fucO"
                     /locus_tag="ACIYSB_001326"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGC5428407.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27334..27981)
                     /gene="fucA"
                     /locus_tag="ACIYSB_001327"
     CDS             complement(27334..27981)
                     /gene="fucA"
                     /locus_tag="ACIYSB_001327"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGC5428408.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            28528..29844
                     /gene="fucP"
                     /locus_tag="ACIYSB_001328"
     CDS             28528..29844
                     /gene="fucP"
                     /locus_tag="ACIYSB_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGC5428409.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            29877..31652
                     /gene="fucI"
                     /locus_tag="ACIYSB_001329"
     CDS             29877..31652
                     /gene="fucI"
                     /locus_tag="ACIYSB_001329"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGC5428410.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            31761..33179
                     /gene="fucK"
                     /locus_tag="ACIYSB_001330"
     CDS             31761..33179
                     /gene="fucK"
                     /locus_tag="ACIYSB_001330"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGC5428411.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            33181..33603
                     /gene="fucU"
                     /locus_tag="ACIYSB_001331"
     CDS             33181..33603
                     /gene="fucU"
                     /locus_tag="ACIYSB_001331"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGC5428412.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            33661..34392
                     /gene="fucR"
                     /locus_tag="ACIYSB_001332"
     CDS             33661..34392
                     /gene="fucR"
                     /locus_tag="ACIYSB_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGC5428413.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(34436..35536)
                     /gene="rlmM"
                     /locus_tag="ACIYSB_001333"
     CDS             complement(34436..35536)
                     /gene="rlmM"
                     /locus_tag="ACIYSB_001333"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGC5428414.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(35529..35924)
                     /gene="ygdD"
                     /locus_tag="ACIYSB_001334"
     CDS             complement(35529..35924)
                     /gene="ygdD"
                     /locus_tag="ACIYSB_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGC5428415.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35943..36860)
                     /gene="gcvA"
                     /locus_tag="ACIYSB_001335"
     CDS             complement(35943..36860)
                     /gene="gcvA"
                     /locus_tag="ACIYSB_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGC5428416.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(37211..37438)
                     /gene="ygdI"
                     /locus_tag="ACIYSB_001336"
     CDS             complement(37211..37438)
                     /gene="ygdI"
                     /locus_tag="ACIYSB_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGC5428417.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            37630..38835
                     /gene="csdA"
                     /locus_tag="ACIYSB_001337"
     CDS             37630..38835
                     /gene="csdA"
                     /locus_tag="ACIYSB_001337"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGC5428418.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            38835..39278
                     /gene="csdE"
                     /locus_tag="ACIYSB_001338"
     CDS             38835..39278
                     /gene="csdE"
                     /locus_tag="ACIYSB_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGC5428419.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(39329..40135)
                     /gene="tcdA"
                     /locus_tag="ACIYSB_001339"
     CDS             complement(39329..40135)
                     /gene="tcdA"
                     /locus_tag="ACIYSB_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGC5428420.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(40374..41471)
                     /gene="mltA"
                     /locus_tag="ACIYSB_001340"
     CDS             complement(40374..41471)
                     /gene="mltA"
                     /locus_tag="ACIYSB_001340"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGC5428421.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            41680..41756
                     /locus_tag="ACIYSB_001341"
     tRNA            41680..41756
                     /locus_tag="ACIYSB_001341"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41714..41716,aa:Met,seq:cat)
     gene            complement(41830..43083)
                     /gene="amiC"
                     /locus_tag="ACIYSB_001342"
     CDS             complement(41830..43083)
                     /gene="amiC"
                     /locus_tag="ACIYSB_001342"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGC5428422.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            43315..44646
                     /gene="argA"
                     /locus_tag="ACIYSB_001343"
     CDS             43315..44646
                     /gene="argA"
                     /locus_tag="ACIYSB_001343"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGC5428423.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(44708..46534)
                     /gene="recD"
                     /locus_tag="ACIYSB_001344"
     CDS             complement(44708..46534)
                     /gene="recD"
                     /locus_tag="ACIYSB_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGC5428424.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(46534..50076)
                     /gene="recB"
                     /locus_tag="ACIYSB_001345"
     CDS             complement(46534..50076)
                     /gene="recB"
                     /locus_tag="ACIYSB_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGC5428425.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYEHHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(50069..52957)
                     /gene="ptrA"
                     /locus_tag="ACIYSB_001346"
     CDS             complement(50069..52957)
                     /gene="ptrA"
                     /locus_tag="ACIYSB_001346"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGC5428426.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKIAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(53133..56501)
                     /gene="recC"
                     /locus_tag="ACIYSB_001347"
     CDS             complement(53133..56501)
                     /gene="recC"
                     /locus_tag="ACIYSB_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGC5428427.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAYLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(56514..56837)
                     /gene="ppdC"
                     /locus_tag="ACIYSB_001348"
     CDS             complement(56514..56837)
                     /gene="ppdC"
                     /locus_tag="ACIYSB_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGC5428428.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56822..57229)
                     /gene="ygdB"
                     /locus_tag="ACIYSB_001349"
     CDS             complement(56822..57229)
                     /gene="ygdB"
                     /locus_tag="ACIYSB_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGC5428429.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(57226..57789)
                     /gene="ppdB"
                     /locus_tag="ACIYSB_001350"
     CDS             complement(57226..57789)
                     /gene="ppdB"
                     /locus_tag="ACIYSB_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGC5428430.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(57780..58250)
                     /gene="ppdA"
                     /locus_tag="ACIYSB_001351"
     CDS             complement(57780..58250)
                     /gene="ppdA"
                     /locus_tag="ACIYSB_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGC5428431.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(58434..59228)
                     /gene="thyA"
                     /locus_tag="ACIYSB_001352"
     CDS             complement(58434..59228)
                     /gene="thyA"
                     /locus_tag="ACIYSB_001352"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGC5428432.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(59235..60110)
                     /gene="lgt"
                     /locus_tag="ACIYSB_001353"
     CDS             complement(59235..60110)
                     /gene="lgt"
                     /locus_tag="ACIYSB_001353"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGC5428433.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(60261..62507)
                     /gene="ptsP"
                     /locus_tag="ACIYSB_001354"
     CDS             complement(60261..62507)
                     /gene="ptsP"
                     /locus_tag="ACIYSB_001354"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGC5428434.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARAKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(62520..63050)
                     /gene="rppH"
                     /locus_tag="ACIYSB_001355"
     CDS             complement(62520..63050)
                     /gene="rppH"
                     /locus_tag="ACIYSB_001355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGC5428435.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(63403..63549)
                     /gene="ygdT"
                     /locus_tag="ACIYSB_001356"
     CDS             complement(63403..63549)
                     /gene="ygdT"
                     /locus_tag="ACIYSB_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGC5428436.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            63735..64424
                     /gene="mutH"
                     /locus_tag="ACIYSB_001357"
     CDS             63735..64424
                     /gene="mutH"
                     /locus_tag="ACIYSB_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGC5428437.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALVGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            64493..65206
                     /gene="ygdQ"
                     /locus_tag="ACIYSB_001358"
     CDS             64493..65206
                     /gene="ygdQ"
                     /locus_tag="ACIYSB_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGC5428438.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            65344..65562
                     /gene="ygdR"
                     /locus_tag="ACIYSB_001359"
     CDS             65344..65562
                     /gene="ygdR"
                     /locus_tag="ACIYSB_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGC5428439.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            65670..66710
                     /gene="tas"
                     /locus_tag="ACIYSB_001360"
     CDS             65670..66710
                     /gene="tas"
                     /locus_tag="ACIYSB_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGC5428440.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYLRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(66742..67935)
                     /gene="lplT"
                     /locus_tag="ACIYSB_001361"
     CDS             complement(66742..67935)
                     /gene="lplT"
                     /locus_tag="ACIYSB_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGC5428441.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(67928..70087)
                     /gene="aas"
                     /locus_tag="ACIYSB_001362"
     CDS             complement(67928..70087)
                     /gene="aas"
                     /locus_tag="ACIYSB_001362"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGC5428442.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            70672..71703
                     /gene="galR"
                     /locus_tag="ACIYSB_001363"
     CDS             70672..71703
                     /gene="galR"
                     /locus_tag="ACIYSB_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGC5428443.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(71710..72972)
                     /gene="lysA"
                     /locus_tag="ACIYSB_001364"
     CDS             complement(71710..72972)
                     /gene="lysA"
                     /locus_tag="ACIYSB_001364"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGC5428444.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            73094..74029
                     /gene="lysR"
                     /locus_tag="ACIYSB_001365"
     CDS             73094..74029
                     /gene="lysR"
                     /locus_tag="ACIYSB_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGC5428445.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(74016..74708)
                     /gene="ygeA"
                     /locus_tag="ACIYSB_001366"
     CDS             complement(74016..74708)
                     /gene="ygeA"
                     /locus_tag="ACIYSB_001366"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGC5428446.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(74837..76255)
                     /gene="araE"
                     /locus_tag="ACIYSB_001367"
     CDS             complement(74837..76255)
                     /gene="araE"
                     /locus_tag="ACIYSB_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGC5428447.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        18661 a        20579 c        18665 g        18444 t
ORIGIN      
        1 tataatgcgc tatttcatac taatgttcac tttcgtatgt tcctttgttg cagcccaacc
       61 aactattgtc ccacaattac agcaacaagt tacggatctt acgagtagct taaactcaca
      121 agaaaagaaa gaactgacgc acaagttaga atctattttc aataataccc aagtgcaaat
      181 tgccgtatta attgttccca caaccaaaga cgagacaatt gaacaatatg ctacaagagt
      241 ttttgacaat tggcgtttag gagatgccaa acgtaatgat gggatactga tcgttgttgc
      301 ctggtcggat cgcactgtcc gcatccaggt aggttatggg ctggaagaaa aggtaaccga
      361 tgctctggct ggagatatca tccgtagcaa catgataccc gcatttaaac aacaaaaatt
      421 agctaaggga ttagagctag ctataaacgc tttgaataac caactcactt cacaacacca
      481 atatccgact aatccttcag aaagtgaatc agcgtcttcc agtgatcatt attactttgc
      541 tattttttgg gtatttgcag tgatgttctt ccctttctgg ttttttcatc aaggcagtaa
      601 tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca atctatcttt tagatttatt
      661 cctgttctcc gataaaattt tttccattgc tgtattttcc ttctttttta ctttcaccat
      721 atttatggtc tttacctgtt tatgtgtact tcagaaaaga gcatctggta gaagttatca
      781 ttcagacaac agcggttccg ctggaggctc agatagcggc ggatttagcg gcgggggcgg
      841 ttcttctgga ggtggcggcg catccggccg ctggtaactc aaccataaaa aatgccagcc
      901 cggaggctgg catttttaaa tcagataaag tcagtcttat gcctggcctt tgatctcttt
      961 acgaccgttg tacggtgctt tttcgcccag agcttcttcg atacgaatca gctggttgta
     1021 tttagcaaca cggtcagaac ggctcataga accagttttg atctggcctg cagcagtacc
     1081 aacagccagg tcagcgatgg tagcgtcttc agtttcgcca gaacggtgag agataactgc
     1141 agtgtagcca gcatctttcg ccatcttgat tgcagccaga gtttcggtca gagaaccgat
     1201 ctggttgaat ttgatcagga tggagttagc gatacctttt tcgatacctt ctttcaggat
     1261 cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc ccagaacttt
     1321 ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga tagaaacgat
     1381 cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg tgaacgcttt
     1441 gttgccttcg ccagccagaa cgtatttacc atctttgtag aattcagaag ctgcgcagtc
     1501 catcgccaaa gtgatgtctt tgcccagttc ataaccagca gctttaacag cttcagcgat
     1561 aacagccaga gcttcagcgt tggaacccag gttcggcgca tagccacctt cgtcaccaac
     1621 agcagtgttc atgcctttcg ctttcagaac ttttgccagg tgatggaaaa cttcagaacc
     1681 catgcggatg gcttctttca cagttttcgc gccaaccggc tgaatcatga attcctggat
     1741 atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg gaaccggcat
     1801 agagtatttg cccggagtac cgttcagttc agcgatgtgc tcgtacagcg gcataccttt
     1861 agcagctgca gcagctttgg cgttagccag agatacagcc aggattgcgt tcgcgccgaa
     1921 tttggatttg ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc cagcctgatc
     1981 tttagcatct ttgccaatca gcgcctgagc gatcgggccg tttaccgcag caacagcttt
     2041 ggttacgcct ttacccagga aacgggattt gtcgccatcg cgcagttcca gagcttcacg
     2101 ggaaccagta gaagcacctg acggagcagc tgccataccg acgaaaccac cctccagatg
     2161 tacttcggct tcaacagtcg ggttaccacg ggagtcgatg atttcacgac cgatgatttt
     2221 tacgattttg gacattaggt tttcctcaag tcactagtta aactgaaact ccagacaaac
     2281 aacgcgtacc cagggtacgc gttgccgctc taactttttt acttacttcg cctgacgttt
     2341 ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat cacgtggagt
     2401 agaagtaaac tccggatgga actggcaagc cacgaaccac gggtgattcg gaacttcgat
     2461 gatctcgacc aactgatcat ccccggaacg gcccgcaacg cgcagacctg catcttcaat
     2521 ctgtttcaac agcatgttgt tgacttcgta acggtgacga tgacgctcaa caattgtcgg
     2581 cgcattgtac agctggcgaa ccaggctatc gtcaaccaac tggcactgct gtgcgccgag
     2641 acgcatggta ccgccgagat cgctcttctc gctacgaact tcaacgttgc cgttttcatc
     2701 gcgccactcg gtaatcagcg ccacaaccgg gtacttacag tctggcacaa attccgtaga
     2761 gttggcgttc tccatgttgg caacatggcg agcgtaatca attaacgcca cctgcatacc
     2821 caggcaaatg cccagataag gaatattgtt ctcacgcgca aaacgcgcgg tcgtaatcat
     2881 gccttctacg ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca gacctttaag
     2941 gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt tgacgctgac
     3001 acgattcttc agcccaccgt gtttcagtgc ttcgatcact gatttataag catccggcag
     3061 ttcaatgtac ttgccgacca taccgatggt gacttcactt accgggttcg cttcttcgaa
     3121 gataacctgt tcccattcgg acagattcgc ttccgggcag tttaagctga atcgtttaca
     3181 aatataatcg tccagcccct gagatttcaa caggcccgga attttataga tggaatcgac
     3241 gtctttcaga gaaataaccg ctttttccgg aacattacag aacaatgcaa tcttcgcacg
     3301 ttcgttcgcc ggaacagcgc gatctgaacg acaaatcagg atgtcaggct ggataccgat
     3361 ggagagcagc tcttttacag agtgctgagt cggtttggtt ttgacttcac cagacgctgc
     3421 catgtacggc accagcgtca ggtgcataaa cagagtgtgc tcacggccaa tttcaacagc
     3481 catctggcga atcgcttcga ggaacggcaa ggattcgata tcacctactg taccgccgat
     3541 ttctaccagt actacgtcat gaccttcgcc accttccagc acgcgctctt tgattgcgtt
     3601 agtgatgtgc ggaataacct gcacggttgc gccgaggtag tcaccgcggc gttctttacg
     3661 cagaacgtca gagtagatac gacccgtggt gaagttgttg cggcggctca ttttggtacg
     3721 aatgaaacgc tcgtagtgcc ccaggtccag gtcggtttca gcgccgtctt cagtaacgaa
     3781 cacttccccg tgttggattg ggctcatagt acctggatcg acgttgatgt acggatccag
     3841 tttcatgatg gtcacattga ggccacgggc ttcaagaatg gctgcgaggg aggctgcggc
     3901 aatgccttta cccagagagg atacgacccc gccggtcaca aaaatatagt tcgttgtcat
     3961 gctgaacctg agaagttagg ttgaaaagac gatggaataa ccaagacggg aaagcagtat
     4021 acccgaacat gacctgtgcc acaaactttc attatccctc ctcttcgcca gcgcactatt
     4081 gaaatcagga gtgagaaaat agcccctttg gggtaaatgt ttttgacgca aatcaagcgc
     4141 ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt aattccctta gagatcaatt
     4201 tcctgccgtt ttacctgttg ccagacttct tccattgttt cgaggtcaac acctgtcatt
     4261 tccagtccac gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc gaatttttcg
     4321 ttcgcttttt gcaatgcgat ttctgctttc gtccctaaat ggcgagccag attaaccgtg
     4381 gcaaacagca ggtcccccat ttcctcctcc agtttagcct ggccgacaac agcctgccgc
     4441 gcttcgtaca tcacctcgtc gatctcttcg tagactttat cgactaccgg accaagcgtc
     4501 gtccaatcga agccaacgtt ggcgcaacgt ttctggattt tttgcgcacg cattaaagcc
     4561 ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc gcgctcttcg
     4621 gttttgattt gctcccaacg ggcaagcact tcactactgt tttcggcaga actatcagca
     4681 aaaacatgcg gatggcgacg ctctaattta tcgctaatag cagcgcaaat atcattaaag
     4741 tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca ccacctggaa tagcagatcg
     4801 cccagttcac cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag cacttcgtag
     4861 gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc ccacgggcag
     4921 ccgttttccg gatcgcgcag gcgctgcata atagtgagca aacggtcgat ttgattcatt
     4981 gaattgtcct gaaaattgcg ggtctgtcag gtggaaacct gtgaccagaa tagaagtgag
     5041 ttagtaacac tacccaatca gtacgttaat tttggcttta atgagttgta attcctctgg
     5101 ggcaactgtt cctttcttcg ttgctcctct tgcccgccag gcgatacttt ttacctgatc
     5161 agctaacgct acgccatcac gttcctgacc ggataaaaca acttcgaacg gatatccttt
     5221 tgattgcgtt gtacaaggaa cacacagaca catacctgtt ttgttgttgt acatgaaagg
     5281 actcaggaca acagctggac gatgtccagc ttgctcgcta ccttttgtcg ggtcaaaatc
     5341 aacccaaatc agatcgccca tatcgggtac gtatcggctt accattacca gacttcctta
     5401 tctttcggct ctccccagtc gatattctcg tggaggtttt ccggcgtgat gtcgttgacc
     5461 agttcagcaa gcgtaaatac gggctcttta cgcactggct caataattaa tttgccatcc
     5521 accaggtcaa tcttcacttc atcatcaata ttcagattga gcgcctgcat taacgtagcc
     5581 gggatccgca ccgccggtga atttccccaa cgctttacgc tactgtggat cataaccctt
     5641 tcctcaaacc gctatcatat gtagatacag tatatatcaa tctacattgt agatacgagc
     5701 aaatttcggc ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5761 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5821 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5881 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagct accacgcgga
     5941 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     6001 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     6061 gcccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     6121 tgtggtgcat caggttgcca acaccttcga ctaccacgcg accgttatct ttactgcggt
     6181 tttgcggcgt gtagcttttt tgctgaagtt gcttcagcgc ggcggcgtcc tgctcttcgg
     6241 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     6301 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     6361 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     6421 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     6481 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     6541 gctgtttctg ggtgataatt tcaatctggt cgcccatctg cagctggtag gtgaacggca
     6601 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     6661 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6721 agacgtacac ccggtcgtca aagacctgac tacgtacttc gtcgagcatt tcgccggaat
     6781 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6841 ccgaacgtgc gccgccagca gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6901 actctgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6961 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     7021 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcatagc
     7081 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcaaagg
     7141 cgaggttctt tttctgcatt ttacgccaga tgctgtagat gtgtttcgga cgaccataca
     7201 cttccgcttt aacgccttca gctttcatct cagcgcgcag atgaccaacg aactcttcga
     7261 tgtagtgttc gcggtcgaga cgccgttcat gcagcagttt ggcaattcgt ttgtattcgg
     7321 ttggatggag gtaacggaag cagtaatctt ccagttccca tttcagttgt ccgattccga
     7381 gacggttagc cagcggtgcg tagatgttgg tacactcttt tgccgccagt acacgttcat
     7441 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     7501 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     7561 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     7621 cgtgaataag gttaacgacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     7681 cgttggcatc cgccagaggg aaaagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7741 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7801 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7861 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7921 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7981 ggcaaatcgc aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     8041 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     8101 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     8161 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     8221 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     8281 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     8341 cggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     8401 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     8461 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     8521 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     8581 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     8641 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     8701 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8761 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8821 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8881 taagtagttc agacttaaac gcgcgcgacg gcgatagccc cagggaacat cggcgatcac
     8941 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     9001 atccacgctg gcgtgttgtt gctgacagcc accgcatacg ccaaaatgag gacagcgtgg
     9061 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     9121 tttatcttca gtaacagtaa cttccgcgtt ttcctgcggc aataatccgg ggataaatag
     9181 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     9241 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     9301 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     9361 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     9421 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     9481 ccggtgccag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     9541 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     9601 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     9661 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9721 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9781 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgctggagc
     9841 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc agcgtgatga
     9901 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9961 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
    10021 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
    10081 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
    10141 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttagat ctggcgaaaa
    10201 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
    10261 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
    10321 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
    10381 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
    10441 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
    10501 acgataaagg gttagaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
    10561 gcgacccgct gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
    10621 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
    10681 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10741 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10801 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10861 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10921 ttattatcga agggccatcc acccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10981 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    11041 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    11101 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    11161 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac
    11221 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctaca cgcctgttgc
    11281 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    11341 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    11401 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcgg
    11461 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    11521 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcaa caaacgccgg
    11581 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    11641 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    11701 acaagcctgg cagcggtatt tcctctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11761 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11821 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11881 ttgaggaaca gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11941 atggcagttg cggctatagc ggtgtgccac gtatgaagaa tctctgccaa ctgatcgaac
    12001 aacagctacg tagcggtact aaagaagaag atttggaacc ggagctgctg gaactgttgg
    12061 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaatgg atgtcttgtc
    12121 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    12181 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    12241 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    12301 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    12361 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    12421 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    12481 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    12541 ttggtcaggc gctgattgcc ttcctgccaa atccagtgcg tatcaatagc agtaatttta
    12601 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgtta
    12661 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12721 tgcattgtcc agaaatgcgg atccgccagc gggatatcaa cggattgcag ggagagcgta
    12781 tggcccattt gccgccagtc ggtggcgatc atattggttg cagtcggtag acctgtcgcg
    12841 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12901 tctgcataag ccagcgaacc tttcaggtat ttaccgattt taatcgcttc gttcagcgac
    12961 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    13021 atagactcgg cctcttcttc cccggccagt acaccgccct tcagtttgaa atcgttgaag
    13081 ccatattttt catatgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    13141 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagcggc
    13201 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    13261 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    13321 attgccgctt ctatcccggt aactacatga atagtggtac gtaggtcaaa tgtctgcaaa
    13381 ccgcgcccac cagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    13441 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    13501 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccag aattatcttt gataatcaca
    13561 atattacgcg taaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    13621 cccgccaccg ggataacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    13681 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13741 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcca ggcagacgtt
    13801 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13861 catcaggaac ggcaattttt ccgtttttaa tctccagcgg attttgggtc aggcgacaat
    13921 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13981 ccacatgggt aaacatcgcc agagagatat cgaaatggtt attagaatgg cagccccagg
    14041 tcagccccca gtcgtcgcaa agctgcgcca cacggactgc accggaaagc gtccagaagt
    14101 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    14161 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aattccgcca
    14221 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    14281 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    14341 caacggtaat ccgcgcatcc gggaagcgtt tcttcaatgc acgaacagtg tcgatttctt
    14401 gctcgccagg taacacgccg cccttaagtt tgaaatcttt aaagccgtag cgatcctgtg
    14461 aggcttccgc cagacgcaca acggcttcgc tgttcattgc tttctgatgc cgcaactgat
    14521 accactcatg gttgcccggc gtattttcca cataaggaag atcggttttg gtccgatcac
    14581 cgatataaaa cagataaccg aggacggtaa tagcctcgcg ttgcttgcct ggccctaaca
    14641 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaagcg gcttccagcg
    14701 ccgccacggc gttaacgcgt aattcaaagg tccaggcacc tttaccgaag gtatcaaaat
    14761 cggcggcctg attaccttta tgcacctgct gaccacttta ttcagtcgcg caacttcctg
    14821 gcccagaacc atcggaatag catcgacaag cgtctgataa atcacatctc cgcccggcgc
    14881 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14941 tgcgttatgt gccccaccaa tattaagcaa catgctgtca tgcccggcca ccggaatgac
    15001 tttcatatca gtaataacag gactggattg tgtcgccatc ttattgcccc gcaacaggtt
    15061 tcaactcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    15121 catgaacacc aacataaatc agcgccccat taaacgagcc agtcgtgcca acgatataac
    15181 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    15241 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    15301 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    15361 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    15421 caatgttcag cgatcccgtg cggcgcatca gccagtcgga aataatccca cccagcacac
    15481 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    15541 tcccgcgtgc ctgcaccaga taaaccggga accaggtaat aaagaagtaa gtcagggcgt
    15601 tgatacagta ctgaccgata taaacgccga tcatcatccg tgaccctagc agctgtttga
    15661 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    15721 tcagcgcacc acccgcggcg atgtactcca gctctttctt atttaccccc ggatgttgat
    15781 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    15841 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15901 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15961 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    16021 cgaccaggaa gcgcagcgta aacagggcaa cgataatgcc gaatccacta aagatatcga
    16081 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    16141 ttgaaccaaa acggtccagc aaccagccac cagggatctg cccgataaca taagcccatg
    16201 agaaagcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    16261 ccgaaccggc gatagagagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    16321 acatcaccac tatccagtaa cgagcatttg tgcgtttttc cacactgctc gcagcctgac
    16381 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    16441 gacatcgcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    16501 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    16561 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    16621 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    16681 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    16741 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    16801 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    16861 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16921 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16981 attcacataa ctactgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    17041 cagactatct ttgattccct gaaagagtgg cacaatgcta tcaggaaggt cgccctgccc
    17101 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    17161 aggatcttca aataccgttg ctttgtggcc ctgcgcggtc agaatcgctt ccgcttcttc
    17221 ggccactaac agtgaattcc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    17281 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    17341 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    17401 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    17461 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    17521 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    17581 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    17641 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    17701 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17761 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17821 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17881 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17941 tgctggccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    18001 cagctgcggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    18061 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccagct
    18121 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    18181 caacggcaaa agcgcccggt aacggctgtt tgttatctaa caaatcgtgc attcgcggga
    18241 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    18301 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    18361 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    18421 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagcgc
    18481 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    18541 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    18601 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    18661 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18721 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18781 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18841 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18901 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18961 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    19021 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    19081 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    19141 agatgacctc taactcttct tccagcgtcg cgataaatag tgtgggcggc agttttagcc
    19201 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    19261 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    19321 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    19381 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    19441 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    19501 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    19561 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    19621 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    19681 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19741 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19801 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19861 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19921 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19981 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    20041 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    20101 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    20161 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    20221 atcaaccaga ttggggttcg ctccaaattc agtatcgtgg acgccaaatt gacagagaaa
    20281 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    20341 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    20401 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    20461 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    20521 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    20581 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    20641 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    20701 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20761 tgctgtctcg ttttgaaaat ttcgatatta acgtcttgcg ccgtgaacgc ggcgtaaagc
    20821 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20881 aggccaactt gttcgcggtc ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20941 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    21001 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    21061 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc ggaaaccagc
    21121 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    21181 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    21241 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    21301 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggtatcatta
    21361 tcttccctgg cggtgtgggt acggcagaag agttgctcta tttgctggga attttaatga
    21421 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    21481 actacttccg cgtactggac gagtttgtcg tgcatacgct gggtgaaaac gcgcgccgcc
    21541 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    21601 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    21661 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21721 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21781 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21841 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21901 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21961 cgtaatctcc gctcccccgg tgacgtttgt tgccgggttt tctcgttttt ggtcacttac
    22021 tcatcaactc atttcatttg ttatatgaat gtttcttacc accctcacgc gacaaatatc
    22081 atcacagtta atatgtcata caatttatgt tgcaacgcaa acgtttccct attttcataa
    22141 accgtatttt atcgctatga aaaagaaatt atcgccatga ttaactaaaa gtattgattt
    22201 tttcagttca acctacatat attgcgcgcc ccggaagaag tcagatgtcg tttaatgggc
    22261 aaatattgcc cttaaattct cttttacttt tgatttacag agtaaagcgt tgggataatc
    22321 tatcttccaa gtagattatt gtatttgaga tcaagatcac tgatagatac ataacttgtg
    22381 tgtatctttc cgccctcaaa ttattacggc ggtaaatgat taagccatcg ccgatagaca
    22441 gatttcattt ttacggtcag gcaccttccc gggctgaact ggctaaaagc tgaattattt
    22501 gcattcctcc aggagaaata gatggaaacg actcaaacca gcacgattgc gtcgaaagac
    22561 tctcgtagtg cctggcgcaa gacagacacc atgtggatgc tgggccttta cggcacggca
    22621 atcggcgcgg gcgtgctgtt cctgccaatc aacgccggtg ttggcggtat gatcccgctg
    22681 atcatcatgg ctatccttgc gttcccgatg acgttttttg ctcaccgcgg cctgactcgc
    22741 ttcgtactgt ctggtaaaaa cccgggcgaa gacatcaccg aggttgtaga agaacacttt
    22801 ggtattggcg caggtaaact gattaccctg ctctacttct tcgctatcta cccgatcctg
    22861 ctggtttata gcgtggcaat caccaatacc gttgaaagct tcatgtctca ccagctgggt
    22921 atgacgccac cgccgcgtgc gattctgtcg ctgatcctga tcgtgggtat gatgaccatc
    22981 gttcgcttcg gtgagcagat gatcgttaaa gcgatgagta ttctggtatt cccgtttgtt
    23041 ggcgtactga tgctgctggc tctgtacctg atcccgcagt ggaacggcgc tgcactggaa
    23101 acgctgtctc tggacactgc atctgcaacc ggaaacggtc tgtggatgac cctgtggctg
    23161 gcaattccgg taatggtgtt ctcgttcaac cactctccga tcatctcttc tttcgccgtt
    23221 gcgaagcgtg aagagtacgg cgatatggca gaacagaaat gctcgaagat cctggcattc
    23281 gcacacatca tgatggtgct gaccgtaatg ttcttcgtct tcagctgtgt actgagcctg
    23341 actccggcag acctggctgc ggctaaagag cagaacatct cgattctgtc ttacctggct
    23401 aaccacttta acgcaccggt tatcgcgtgg atggctccga ttatcgcgat tatcgctatc
    23461 accaaatcct tcctcggtca ctacctgggc gcacgtgaag gcttcaacgg tatggtgatt
    23521 aaatctctgc gtggtaaagg taagtctatc gaaatcaaca agctgaaccg tatcactgcg
    23581 ctgttcatgc tggtaacgac ctggattgtt gccaccctga acccgagcat cctgggtatg
    23641 attgaaaccc tgggcggtcc aatcatcgcg atgatcctgt tcctgatgcc gatgtacgca
    23701 attcagaaag taccggcaat gcgtaagtac agcggtcaca tcagcaacgt attcgttgtc
    23761 gtgatgggtc tgattgcaat ctccgcaatc ttctactctc tgttcagcta agtcctttcg
    23821 cgccgctttc gggcggcgct tcctccgttt taacgcgatg tatttcctat gattagcgta
    23881 ttcgatattt tcaaaatcgg cattggccct tccagttctc ataccgttgg accaatgaaa
    23941 gcgggtaaac aatttaccga cgatctgatt gcccgtaacc tgcttaaaga cgtgacccgc
    24001 gtggtggttg acgtgtacgg ctcgctctct ctgaccggta aaggccacca cactgatatc
    24061 gccattatta tgggcctggc gggtaacctg ccggataccg tggatatcga ttccatcccc
    24121 agttttattc aggatgtgaa tactcatggt cgcctgatgc tggcaaacgg tcagcatgaa
    24181 gtggagttcc cggttgatca gtgcatgaac ttccacgccg acaacctttc tctgcatgaa
    24241 aacggtatgc gcattaccgc gctggcgggc gataaagtcg tttacagcca gacttactac
    24301 tctattggcg gtggctttat cgttgatgaa gagcattttg gccagcagga tagcgcaccg
    24361 gttgaagttc cttatccgta cagttcagca gccgatctgc aaaaacattg tcaggaaacc
    24421 gggctgtcac tctctggcct gatgatgaaa aacgagctgg cgctgcacag caaagaagag
    24481 ctggaacagc acctggcgaa cgtctgggaa gtcatgcgcg gcggtattga gcgcggtatt
    24541 tccaccgaag gcgtgttgcc tggcaaactg cgcgttccac gccgtgctgc ggcactacgc
    24601 cggatgctgg tcagccagga taaaaccacc actgacccga tggcggttgt tgactggatc
    24661 aacatgtttg cactggcagt gaacgaagag aacgctgctg gcggtcgcgt ggtgactgcg
    24721 ccgactaacg gtgcgtgcgg gattatcccg gcagttctgg cgtactacga caagtttatc
    24781 cgcgaagtga acgctaactc actggctcgt tacctgctgg tagccagcgc cattggttct
    24841 ctttataaga tgaacgcgtc gatttctggt gctgaagtgg gttgccaggg tgaagttggc
    24901 gtggcgtgct caatggcggc ggctggtctg gcagaactat taggcgcaag cccggcgcag
    24961 gtgtgcatcg cggcggaaat cgccatggag cacaacctcg gtctgacgtg tgacccggtc
    25021 gccggacagg tacaggtgcc atgcatcgag cgtaacgcca ttgcggcagt aaaagcggtg
    25081 aacgccgcac gtatggcgct gcgccgtacc agcgagccgc gcgtctgcct cgataaagtt
    25141 atcgaaacca tgtacgaaac aggtaaagat atgaacgcca agtaccgcga aacctctcgc
    25201 ggcggcctgg caatgaagat cgttgcctgc gattaatcgc tctccagagg cctcgttttg
    25261 cgaggcctct tcccgatttc tcatccagcc gtagcctgtt ccggcatcga atgttaccct
    25321 tatcgcctga tctttaaggg ggttatcgtg gctgttcatt tgcttattgt cgatgcactg
    25381 aatcttattc gtcgcattca tgccgttcag gggtcgccct gtgtcgaaac ctgccagcat
    25441 gcgctcgatc agctcattat gcacagccag ccaacccacg cggtcgccgt ttttgatgat
    25501 gaaaaccgca gtagcggctg gcgtcatcag cgtttaccag attacaaagc gggtcgaccg
    25561 ccaatgccgg aagagttgca cgacgagatg cctgcattac gcgccgcctt tgagcaacgc
    25621 ggcgtcccgt gctggtcaac cagcggcaac gaagccgatg acttagccgc cacgctggcg
    25681 gtcaaagtga cacaggccgg gcatcaggca acgattgttt cgacagataa aggctactgt
    25741 cagttacttt caccgacatt acgtattcgt gattacttcc agaaacgttg gctggatgcg
    25801 ccatttatcg ataaagaatt tggcgttcaa ccgcagcagt tgcccgatta ctggggactt
    25861 gcggggatca gcagttcaaa ggtaccgggt gttgcgggaa tcggaccaaa aagcgccacg
    25921 cagctgctgg tcgagtttca gagtctggaa gggatatatg agaatctgga tgcggttgcc
    25981 gaaaagtggc gcaaaaaatt agaaacccat aaagagatgg cgtttctgtg ccgcgatatt
    26041 gcccgcttac aaaccgattt gcatatcgac ggcaatttac agcaattgcg gttggtacgg
    26101 taacggcgag ccggatacgc cgcaaacgtc gtatccggca ttatcacatc agcgcattta
    26161 ccaggcggta tggtaaagct ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc
    26221 ggtacaaaca tcatccagtg ccgcctgcgc cagtgccgga atgtcttcct tgcgtacacc
    26281 aacatcacgc aaatgtggcg gaataccgac atcacggttg agagcaaaca ccgcttcaac
    26341 agcggcatta cgcgcctctt ccaggctcat accttccact ttcacgccca taacgcgcgc
    26401 gatatcgcgg tacttctcac cggtaaagtc agcgttataa cgcatgacat gcggtaacag
    26461 gatggcgttc gcaacaccgt gtggagtgtt ataaaacgcg cccagtggat gcgccatacc
    26521 atgcaccaac cctaacccaa cattcgagaa gcccataccc gcaacatact gcccgagcgc
    26581 catttcttct ccggcatcct tatcaccagc aaccgatcct cgcagcgccc cagcaatgat
    26641 ttcaatcgct ttaatgtgca gtgcatcggt tagcgcccac gcgccacggg taatataccc
    26701 ctcaatagca tgagtgagcg catcgacacc cgtcgcagct ttcagcgctg gaggcatacc
    26761 atccatcatg tcagcgtcaa taaacgccac ctgcgggata tcatgcggat caacgcaaac
    26821 aaacttgcgc cgtttctctt cgtcagtgat cacgtagtta atggtcactt ctgccgcagt
    26881 acctgctgtg gtaggaattg ccagaatcgg tacactgggt ttattggtcg gggaaagccc
    26941 ttccaggcta cgcacatcgg caaactccgg gttgttgctg ataatgccaa tcgctttaca
    27001 agtatcctgt ggagaaccac caccaatagc gatcaggtaa tccgcgccgc tattctggaa
    27061 tacaccgagc ccttctttga cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat
    27121 cgcccatgcc agccctgcag catccatctt atcggtcact ttcgccacca cgccgcattg
    27181 caccagcgtt ttatcggtga cgatcagcgc cttctgataa ccacggcgtt tcacctcatc
    27241 ggttaaagcc ccaacagcac cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt
    27301 agccatcatc cttctccttg ttgctttacg aaattactct tcaattcgta acccataggt
    27361 tttgaatttc tccagcacta cggcaatctc ttcatcgctc agcactggca ccgggtccgt
    27421 aatcgccagg gtcgtcaggt aaagttgcgc cagcacttca acttcatgcg ccagccataa
    27481 cgctttttcc agattcacct cacaagcgat aagcccatga tgttgtaaca aagttgcctt
    27541 acgatttttg agagccagcg caacatgttc agaaagttcg cgtgttccaa aggtcgcata
    27601 aggcgcgcaa ggaatagaat taccgccagc cgccgcaatc atgtagtgaa tagcggggat
    27661 cgatcggtta agaatggaaa ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt
    27721 ggcatccggt ctgctttgat aggctgccat atggaaacgc cattcgcttg aggggagctt
    27781 tccttcctca tgtttaccgt tgccatcaat aaagacaata tgcgactccg tcagtttttc
    27841 atatggaatg cctgtaggcg taatcagcat cccatcctga taacgtacac tgacgttccc
    27901 cgctgtcccc tggttcagtc ccaggcgggt catttccagg caagtgtcaa taatctgacg
    27961 agcaagttta tttcgttcca ttagctacct ctctctgatt caaaacaggg caataatgtt
    28021 gttcctttca cactattgaa ttagccgttt aattacccac catcttcttc ctgattaaca
    28081 agaaagaaat tcacaagctt atattttgtg acctggttca actaatcaca gtaaataact
    28141 gcaagttctc tttttataac cccattaaaa atgaccgctc ttaaaaatat ttatcaaaac
    28201 ggtcattttt ctattcctcc aagcccggaa tgaccgtttt cggcacaaac aattaatacg
    28261 gtcatctgat ttgtgttttt tatgatttat tttctgaaac gggcatgaaa tttcgattat
    28321 taaagtgatg gtagtcacat aaagtcacct tctagctaat aagtgtgacc gccgtcatat
    28381 tacagagcgt tttttatttg aaaatgaatc catgagttca tttcagacag gcaaatattc
    28441 actgatatga agcccgaact cgctggtttt gcacttttga aaacataacc gattacgtgc
    28501 ttaagcttct gaacctaaga ggatgctatg ggaaacacat caatacaaac gcagagttac
    28561 cgtgcggtag ataaagatgc agggcaaagc agaagttaca ttattccatt cgcgctgctg
    28621 tgctcactgt tttttctttg ggcggtagcc aataacctta acgacatttt attacctcaa
    28681 ttccagcagg cttttacgct gacaaatttc caggctggcc tgatccaatc ggccttttac
    28741 tttggttatt tcattatccc aatccctgct gggatattga tgaaaaaact cagttataaa
    28801 gcagggatta ttaccgggtt atttttatat gccttgggtg ctgcattatt ctggcccgcc
    28861 gcagaaataa tgaactacac cttattttta gttggcctat ttattattgc agccggatta
    28921 ggttgtctgg aaactgccgc aaaccctttt gttacggtat tagggccgga aagtagtggt
    28981 cacttccgct taaatcttgc gcaaacattt aactcgtttg gcgcaattat cgcggttgtc
    29041 tttgggcaaa gtcttatttt gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa
    29101 atgtctccag agcaattgag tgcgtataaa cacagcctgg tattatcggt acagacacct
    29161 tatatgatca tcgtggctat cgtgttactg gtcgccctgc tgatcatgct gacgaaattc
    29221 ccggcattgc agagtgataa tcacagtgac gccaaacaag gatcgttctc cgcatcgctt
    29281 tctcgcctgg cgcgtattcg ccactggcgc tgggcggtat tagcgcaatt ctgctatgtc
    29341 ggcgcacaaa cggcctgctg gagctatttg attcgctacg ctgtagaaga aattccaggt
    29401 atgactgcag gctttgccgc taactattta accggaacca tggtgtgctt ctttattggt
    29461 cgtttcaccg gtacctggct catcagtcgc ttcgcaccac acaaagtcct ggccgcctac
    29521 gcattaatcg ctatggcact gtgcctgatc tcagccttcg ctggcggtca tgtgggctta
    29581 atagccctga ctttatgcag cgcctttatg tcgattcagt acccaacaat cttctcgctg
    29641 ggcattaaga atctcggcca ggacaccaaa tatggttcgt ccttcatcgt tatgaccatt
    29701 attggcggcg gtattgtcac tccggtcatg ggttttgtca gtgacgcggc gggcaacatc
    29761 cccactgctg aactgatccc cgcactctgc ttcgcggtca tctttatctt tgcccgtttc
    29821 cgttctcaaa cggcaactaa ctgaacatat tttccgaata aagtgaggaa tctgtaatga
    29881 aaaaaatcag cttaccgaaa attggtatcc gcccggttat tgacggtcgt cgcatgggtg
    29941 ttcgtgagtc gcttgaagaa caaacaatga atatggcgaa agctacggcc gcactgctga
    30001 ccgagaaact gcgccatgcc tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta
    30061 tcgcgggtat ggctgaagcc gctgcttgcg aagaaaaatt cagcagtcag aatgtaggcc
    30121 tcaccattac ggtaacgcct tgctggtgct atggcagtga aaccatcgac atggatccaa
    30181 cccgcccgaa ggccatttgg ggctttaacg gcactgaacg ccccggcgct gtttacctgg
    30241 cagcggctct ggcagctcac agccagaaag gcatcccagc attctccatt tacggtcatg
    30301 acgttcagga tgccgatgac acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct
    30361 ttgcccgcgc cggtttggcc gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg
    30421 gcgtttcgat gggtatcgcc ggttccattg ttgatcacaa cttctttgaa tcctggctgg
    30481 gaatgaaagt ccaggcggtg gatatgaccg aactgcgtcg ccgtatcgat cagaagattt
    30541 acgacgaagc cgaattggaa atggcactgg cctgggctga taaaaacttc cgctatggcg
    30601 aagatgaaaa taacaaacag tatcaacgta atgccgagca aagccgcgca gttctgcgcg
    30661 aaagtttact gatggcgatg tgtatccgcg acatgatgca aggcaacagc aaactggccg
    30721 atattggtcg cgtggaagaa tcacttggct acaacgccat cgctgcgggc ttccaggggc
    30781 aacgtcactg gaccgatcaa tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt
    30841 catttgactg gaatggcgtg cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta
    30901 acggcgtggc aatgctaatg ggtcaccagc tcaccggcac cgctcaggta tttgccgatg
    30961 tgcgtaccta ctggtcacca gaagcaattg agcgtgtaac ggggcataaa ctggatggac
    31021 tggcagaaca cggcatcatc catttgatca actccggttc tgctgcgctg gacggttcct
    31081 gtaaacaacg cgacagcgaa ggtaacccga cgatgaagcc acactgggaa atctctcagc
    31141 aagaggctga cgcttgcctc gccgctaccg aatggtgccc ggcgatccac gaatacttcc
    31201 gtggcggcgg ttactcttcc cgcttcctta ccgaaggcgg cgtcccgttc accatgactc
    31261 gtgtcaacat catcaaaggc ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg
    31321 aattgccgaa ggatgtgcat gacatcctca acaaacgcac caactcaacc tggccaacca
    31381 cctggtttgc accgcgcctc accggtaaag ggccgtttac ggatgtgtac tcggtaatgg
    31441 cgaactgggg cgctaaccat ggggttctga ccatcggcca cgttggcgca gactttatca
    31501 ctctcgcctc catgctgcgt atcccggtat gtatgcacaa cgttgaagag accaaagtgt
    31561 atcgtccttc tgcctgggct gcgcacggca tggatattga aggccaggat taccgcgctt
    31621 gccagaacta cggtccgttg tacaagcgtt aatacattac tccctgatgt gatgcccggt
    31681 cgctccggct accgggcctg aacaagcaag agtggttagc cggataagca atgttatccg
    31741 gctatattgc aggagcgatt atgaaacaag aagttatcct ggtactcgac tgtggcgcga
    31801 ccaatgtcag ggccatcgcg gttaatcggc agggcaaaat tgttgcccgc gcctcaacgc
    31861 ctaatgccag cgatatcgcg atggaaaaca acacctggca ccagtggtct ttagacgcca
    31921 ttttgcaacg ctttgctgat tgctgtcggc aaatcaatag tgaactgact gaatgccaca
    31981 tccgcggtat cgccgtcacc acctttggtg tggatggcgc tctggtagat aagcaaggca
    32041 atctgctcta tccgattatt agctggaaat gtccgcgaac agcagcggtt atggacaata
    32101 ttgaacggtt aatctccgca cagcggttgc aggctatttc tggcgtcgga gcctttagtt
    32161 tcaatacgtt atataagttg gtgtggttga aagaaaatca tccacaactg ctggaacgcg
    32221 cgcacgcctg gctctttatt tcgtcgctga ttaaccaccg tttaaccggc gaattcacta
    32281 ctgatatcac gatggccgga accagccaga tgctggatat ccagcaacgc gatttcagtc
    32341 cgcaaatttt acaagccacc ggtattccac gccgactctt ccctcgtctg gtggaagcgg
    32401 gtgaacagat tggtacgcta cagaacagcg ccgcagcaat gctcggctta cccgttggca
    32461 taccggtgat ttccgcaggt cacgataccc agttcgccct ttttggcgct ggtgctgaac
    32521 aaaatgaacc cgtgctctct tccggtacat gggaaatttt aatggttcgc agcgcccagg
    32581 ttgatacttc gctgttaagt cagtacgccg gttccacctg cgaactggat agccaggcag
    32641 ggttgtataa cccaggtatg caatggctgg catccggcgt gctggaatgg gtgagaaaac
    32701 tgttctggac ggctgaaaca ccctggcaaa tgttgattga agaagctcgt ctgatcgcgc
    32761 ctggcgcgga tggcgtaaaa atgcagtgtg atttattgtc gtgtcagaac gctggctggc
    32821 aaggagtgac gcttaatacc acgcgggggc atttctatcg cgcggcgctg gaagggttaa
    32881 ctgcgcaatt acagcgcaat ctacagatgc tggaaaaaat cgggcacttt aaggcctctg
    32941 aattattgtt agtcggtgga ggaagtcgca acacattgtg gaatcagatt aaagccaata
    33001 tgcttgatat tccggtaaaa gttctcgacg acgccgaaac gaccgtcgca ggagctgcgc
    33061 tgttcggttg gtatggcgta ggggaattta acagcccgga agaagcccgc gcacagattc
    33121 attatcagta ccgttatttc tacccgcaaa ctgaacctga atttatagag gaagtgtgaa
    33181 atgctgaaaa caatttcgcc gttaatttct cccgaactat tgaaagtgct ggcagagatg
    33241 ggacatggag atgaaattat tttttccgat gctcactttc ccgcccattc gatgggaccg
    33301 caggtgatcc gcgctgatgg cctgttggtg agcgacttgc tccaggcgat tatcccgtta
    33361 tttgaactgg acagttatgc accgccgctg gtgatgatgg cggcggtaga aggtgacact
    33421 ctcgatcctg aagtagaacg acgttaccgt aatgcgcttt cactacaagc cccgtgtcct
    33481 gacatcatcc gcatcaatcg ttttgcgttt tatgaacggg cgcaaaaagc ctttgcgatc
    33541 gttatcacag gcgaacgagc gaagtacggg aatattcttt taaaaaaagg ggtaacaccg
    33601 taatctcata ccggtacgcc cgcatgacgc gggcggttat cgaatgatgg ggtgaaaaat
    33661 atgaaagcgg cacgccagca agcgatagtc gacctgctgc tgaaccatac cagcctgacc
    33721 acggaagctc tctctgaaca gctaaaggtc agtaaagaaa ccattcgtcg cgatctcaat
    33781 gaattacaga cgcagggtaa aattctgcgc aatcatggac gcgctaaata tatccaccgt
    33841 caaaatcaag acagtggcga tccctttcac atcaggctga aaagccatta tgcgcataaa
    33901 gcagatatcg cgcgcgaggc gctcgcgtgg attgaagaag ggatggtgat agccttagac
    33961 gccagttcaa cttgctggta tctggcacgc cagttgcctg acatcaacat tcaggtcttc
    34021 accaatagcc atccgatttg ccatgaactc ggtaaacgcg aacgcattca actgatcagt
    34081 tccggcggca cacttgagcg caaatatggc tgttacgtca atccctcgct gatttcccaa
    34141 cttaaatcgc tggaaatcga tctgtttatt ttttcttgtg aagggatcga tagcagcggc
    34201 gcactgtggg actccaatgc gatcaacgct gattacaaat cgatgctatt aaaacgtgcc
    34261 gcgcaatcgt tgttattgat tgataaaagt aaatttaatc gttcagggga agcccgcatc
    34321 gggcatctgg atgaggtaac gcacattatt tctgatgagc gccaggttgc aacttctttg
    34381 gtaacagcct gacggaaagg gtagcaggcc ggagacgacg ccccggcctt gcctgttatc
    34441 gctcgtcgcg acgaccaccc accgcagccc agatgcggcg gacgtgcacc gtcacttctt
    34501 cgcgatcgtg atacaactgc cgcgcctgaa tctgagcatt tatgccatgt tcatcaagct
    34561 gtgcctgaat atacgccaga ttgtgtgaca cttcttcgta gcgttttttc atcggcagtt
    34621 tgaggttgaa aatggtttca cggcaccagc cattaaccag ccactgcgcc atcaatgccg
    34681 caactttcgc cggtttttca accatatcgc ataccatcca ggagatattg ctgcgcgtcg
    34741 gacggaattt gaaaccgtct tcccgcagcc acgtcacctg tccggtatcc atcagacttt
    34801 gcgccatcgg gccgttgtcg acggaataaa cccacatgtt gcgcttcacc agttggtagg
    34861 tccagccgcc agggcaagcg cctaaatcca ccgcccacat cccgttcgcc aggcgttcat
    34921 cccactcatc cgcaggaata aacacatgaa atgcctcttc cagtttgagc gtggaacgac
    34981 tcggcgcatc tgccggaaat ttcaggcgcg gaatgcccat atagaacggc gaattattgt
    35041 tgctgtatga gtaaccggta tagcagcagc ctggtgcaat aaagaataca tgcacaaccg
    35101 gacgcttcgg cgtttcatag ttcgccagca ccccggcatc gcgcaaggca gcgcgtagcg
    35161 gaacagtaaa tttacggcag aatttcagta actctttgct ttcgttggta tcggcaactt
    35221 caacacgcag ttcaccgccc ttctctacta cgccctgtaa catgccgaca atgggggtaa
    35281 tacgatcttc tggcggcaaa tgctgcagga gttcccccac cacaaaccac tggcgggcaa
    35341 aaattaatga actgaacggc agctcacgga ttaacttatc gccatcatca ggttgataac
    35401 attcataaat gacataaccc gcattctctt tcacgcgggc aaaaccgaaa atttcccgct
    35461 ggccggcttt atcggtaatt tctgcggcgc actctttttc aaagcccgga cggcacagca
    35521 atacaacctt attcatgact tacgcccttg cgctttaaac ggatagcacc aactaacatt
    35581 aacgcccagc ccgcgaggaa gctcacgccg ccaaccggag tgacaaacgc ccacaaacgc
    35641 agatgggaca gcgccaggca ataaaggctg ccgctgaaca acaccgtgcc taacgcgagg
    35701 aaaacgctac tccagtaaaa ccagatactg atgcgacgct gcattgccac cgccagacct
    35761 aagatcgcca gcgtatgaaa cgcctggtat tcgaggccgg tctggatcca gcccatctca
    35821 acggccccca tggttttact taacacatgc gcgccaaaag cgcccagagc cacaaaaatg
    35881 aagccgctaa tggcggcgaa aatcagcata aaacggctgg tcatggtcgt accctacgta
    35941 aattattgtt cataacgaaa gcggaatttt tcttgttcag cagcggcttt cgccaggatc
    36001 cattggcgaa aggcggctat tttacccagt tctgcctgac tgtcatgaca aaccagataa
    36061 aaagcatttt tactgaccag aacatcatta aacgggcaaa caagacgtcc ggcctcgatt
    36121 tcagattgcg ccatcacgtt atttgccagc gccactccct gcccgtggat agccgcttgc
    36181 agcaccatgg cgctatggct aaaaattggc ccttgctgaa cgttgatatg atttaacccc
    36241 aactgtcggg tatatgtctg ccagtcacgg cgcgaagcat catgtaataa cgtatgttta
    36301 gccagatctt ccggtgtctt caagggtttt tcgccagtca gcagtagcgg cgaacacacc
    36361 ggcaataaat attcggcgta cagtttttcc acccgtagcc ccggccagtt gccccgacca
    36421 taaaatatcg ccacatcaac atcatccgcc agcttatctt cctgacgatc aaccgcctgg
    36481 attcgaacgt caattcccgg ataagctgaa ttaaagctgg aaagtcgcgg aaccaaccaa
    36541 tgaatggcga aactggggag taaactgacc gtcaacgccc ccttggcgct acgggcctgg
    36601 agtttacgcg tcgcttcggt taattgcgaa aatatctctt tgatatcgag gaaatagctt
    36661 tgcccttcct cggtcagcag gagtgaacga ttacggcggc ggaacagttt tagccccaaa
    36721 aaatcctcaa gagacttgat ttgatgactt actgcggctt gggtcacaaa aagctcttct
    36781 gctgcgcgag tgaaacttaa atggcgtgct gcggcatcaa aaactcgtaa ggcatttagc
    36841 ggtggtaatc gtttagacat ggctattaaa ctttgatgtt aaatgaattt aacaattaga
    36901 tcacactatg taacctatta gtttttttaa tctgagccat tataaattgt ccgttgagct
    36961 tctaccagca aatacctata gtggcggcac ttcctgagcc ggaacgaaaa gttttatcgg
    37021 aatgcgtgtt ctggtgaact tttggcttac ggttgtgatg ttgtgttgtt gtgtttgcaa
    37081 ttggtctgcg attcagacca tggtagcaaa gctacctttt ttcacttcct gtacatttac
    37141 cctgtctgtc catagtgatt aatgtagcac cgcctaattg cggtgctttt ttttaccttg
    37201 cgatcgcgaa ttactgatcc agttcgacca tctctttcac gtcagtacgg ttgatctgct
    37261 gtttgttgcc attagcgtct ttatacgaaa tcataccggt atcgttatca gtctgtggtt
    37321 tgccgtcaga gacgatggta cgtccgtcat tggtgtgcat cacatagttc gaaccggaac
    37381 aggcgctcag ggcaaaagtc agcatacagg cagaaataat tgcggcagtc tttttcatca
    37441 tcttctcctt aaagcgatta ataaatctta atagtattga ttaacaggct aaaattaacg
    37501 cctaacacta ttcagcatat gttacttacg cggcttcgcc aggatatcca gataattctg
    37561 atggttagca ctctccttgt atcaaagtga attttgcgtc acgatcggtg catcaagccg
    37621 aggagtacca tgaacgtttt taatcccgcg cagtttcgcg cccagtttcc cgcactacag
    37681 gatgcgggcg tctatctcga cagcgccgcg accgcgctta aacctgaagc cgtggttgaa
    37741 gccacccaac agttttacag tctgagcgcc ggaaacgtcc atcgcagcca gtttgccgaa
    37801 gcccaacgcc tgaccgcgcg ttatgaagct gcacgagaga aagtggcgca attactgaat
    37861 gcaccggatg ataaaactat cgtctggacg cgcggcacca ctgaatccat caacatggtg
    37921 gcacaatgct atgcgcgtcc gcgtctgcaa ccgggcgatg agattattgt cagcgtggca
    37981 gaacaccacg ccaacctcgt cccctggctg atggtcgccc aacaaactgg agccaaagtg
    38041 gtgaaattgc cgcttaatgc gcagcgactg ccggatgtcg atttgttgcc agaactgatt
    38101 actcctcgta gtcggattct ggcgttgggt cagatgtcga acgttactgg cggttgcccg
    38161 gatctggcgc gagcgattac ctttgctcat tcagccggga tggtggtgat ggttgatggt
    38221 gctcaggggg cagtgcattt ccccgcggat gttcagcaac tggatattga tttctatgct
    38281 ttttcaggtc acaaactgta tggcccgaca ggtatcggcg tgctgtatgg taaatcagaa
    38341 ctgctggagg cgatgtcgcc ctggctgggc ggcggcaaaa tggttcacga agtgagtttt
    38401 gacggcttca cgactcaatc tgcgccgtgg aaactggaag ctggaacgcc aaatgtcgct
    38461 ggtgtcatag gattaagcgc ggcgctggaa tggctggcag attacgatat caaccaggcc
    38521 gaaagctgga gccgtagctt agcaacgctg gcggaagatg cgctggcgaa acgtcccggc
    38581 tttcgttcat tccgctgcca ggattccagc ctgctggcct ttgattttgc tggcgttcat
    38641 catagcgata tggtgacgct gctggcggag tacggtattg ccctgcgggc cgggcagcat
    38701 tgcgctcagc cgctactggc agaattaggc gtaaccggca cactgcgcgc ctcttttgcg
    38761 ccatataata caaagagtga tgtggatgcg ctggtgaatg ccgttgaccg cgcgctggaa
    38821 ttattggtgg attaatgaca aacccgcaat tcgccggaca tccgttcggc acaaccgtaa
    38881 ccgcagaaac gttacgcaat accttcgcac cgttgacgca atgggaagat aaatatcgcc
    38941 agttgatcat gctggggaaa cagcttccgg cattgccaga cgagttaaaa gcgcaggcta
    39001 aagagattgc cggatgcgaa aaccgcgtct ggctgggata tacagtggct gaaaacggca
    39061 aaatgcattt ctttggcgac agcgaagggc gcattgtgcg cggcctgctg gcggtgttgt
    39121 tgactgccgt tgaggggaaa accgccgccg agttgcaggc acagtcacca ctggcattgt
    39181 ttgatgagct gggattacgt gcgcagctta gcgcctcacg cagccagggg ttaaatgcgt
    39241 taagcgaggc gattatcgct gcgacgaagc aggtttaagc acagaaccgg gtcggataag
    39301 acgttcgcgt ctcatccgac ctgattgttt aaccctgacg cgccgctttc gccatcatct
    39361 tcttcagcgc atgagaaacc gcaacaaacc caaaggtggc ggtcaccatc gttgccgcgc
    39421 caaatccaga tgcacaatcc atccgcttcg gcccttctgc cgtggctttc atcgcacaca
    39481 ccgtaccgtc tgactgcggg tacaccagcg cttcagtaga aaacacgcaa tccacgccga
    39541 gcttaccttt actgtttttc actacgccaa aatcgctttt caggcgctcg cgcaacttcg
    39601 ccgccagcgg gtcctgaatc gttttcgcca gatcggtaac ctgaatctgc gtcggatcaa
    39661 tctgcccacc cgcgccaccg gttgtgacca gtgggatttt attgcgccga caataagcaa
    39721 tcagcgccgc tttgggccgt acactatcaa tggcatcaat cacgtacgaa taacccacgc
    39781 tcatatactg cgctacgtta tccggcgtca cgaaatcatc caccaccgtt acacggcact
    39841 ccgggttaat ctggcgaata cgctccgcca taacttccgc ttttgccagc ccaacgttat
    39901 cgcgcagggc atgaatttgc cgattggtat tggtgacgca cacatcatcc atatcgataa
    39961 gcgtgattgc gccaatcccc gtgcgcgcca gcgcttccgc cgcccaggaa ccgacaccac
    40021 cgataccaac cacacaaata tgcgcgtcag caaacagttg caacgctttt tcaccataca
    40081 gacgcgctgt gccaccaaaa cgctgacgcc atgcatcact aattaccaca gacatagcac
    40141 ctcagaatta aaaagggcga ggttatccct caccctaaac gcataaatgc ctgatgcgct
    40201 acgcttatca ggcctacata actcctgcaa tattttggat ttgcatgatt tgtaggccgg
    40261 ataaggcgtt cacgccgcat ccggcatgaa caacgcgcac ttagtcaata atcaggatgt
    40321 gaggttaccc ctcaccctgt catatccgta aaaacggcat acagaatatc acatcagccg
    40381 ctaaagacgt tacctgcgcc cggggcggtt ttcagcaccc agacacgtcc atagtggttg
    40441 taccaacctg cgcggtgtcc ggcttccggc ccgatccctt gatagatatc gaagtgttgg
    40501 cctttgattg caccaccgac atccagcgcc accatcagac gcagttcgta ctgaccatta
    40561 aatttgccgt tattatccag caacggcact tctgccagca aggtagtacc tggcggaata
    40621 atggaacgat cagaggcaac tgacgcgcga ccaaccagcg gcaccgcact tgcccctttc
    40681 accggagcaa aagattgcgg tttaaagaag acgaaagacg ggttctgttc cagcagctcg
    40741 cgaacctcgg cttcactgtg tgtttcgccc cagtgacgaa tcgcctgcat cgacatatct
    40801 tcttttttca cttcgccacg gtcgatcagc accttaccaa tgctgcgata ggcatgaccg
    40861 tttttccctg catagctgaa aaagttaagc ggactgccat caccaaagtc gatataccca
    40921 ctaccctgca catccataat gaagttatcc atcagggagt tactgtaagc gagaatatat
    40981 ttatcactca atgcccccgc gtagatctcc gcacgagacg gcagacgacc acgttttggc
    41041 ggcatacggt aaataggata ctggaactcg ccctggcggg tatggcgcgc ctgaattacc
    41101 ggcgtgtaat aaccggtaaa ctgcacgtta ccatagttgt cggcaccttc catctgccag
    41161 gcatcaatgc cgaactggcg catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc
    41221 accgcgttat aaacattact ctggttgcca tacagacgcg gtgacgaatt acggatatgg
    41281 ttaatttgct cggcaaaatc accggcgtta atcggcgcgc caacggcatc tggctggttc
    41341 accagagaga aaggctgggt aaatttcccg tctttatatt gctgtccgcg atcggttggt
    41401 ttggaagagc aggcggcaag cattgccaca accgtgccca taagaaggta ctttacccaa
    41461 cgtcctttca ttgttctctt cttcaggtta aaaaataagg cgcaacgaag ataacaaacc
    41521 gccgcgttta atgaaatgag cagcctctcc ctgacgcaaa ttttgcacaa aaaataggct
    41581 ttagtgattt gtttttgttc aaaatcatgc caaatccgtg atcggggtaa aaaaaaggtt
    41641 gcatgaaaac gcgagcggag tatagtgcgc atccacggac gcggggtgga gcagcctggt
    41701 agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat ccggcccccg caaccaatta
    41761 ttgaacaccc taacgggtgt ttttttgttt ctggtctccc ataaaaaagc gccattcagc
    41821 gcctttttat catccccttc tcgccagcgt cgccccatcg gcaaaatacg ctttaatccc
    41881 cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc gttttcagtt tacgctcttc
    41941 ctcaacgtta ctgataaacg ccgtttcgac cagaatggag ggaatatctg gtgcctttag
    42001 tacggcaaac ccggcctgtt caacttgatt tttatgcagc ttgttgattt taccgagctt
    42061 attcagtacc gctttaccaa acttcaggct gtcggcaatg gtcagcgact gtaccatatc
    42121 gaacatggtg tggtcgacat agcggtcacc gcttttgctc acgccaccaa tcaagtccga
    42181 ggcgttctgg gtttgtgcca gatattttgc cgcagtactg gttgcacctt tggttgagag
    42241 cgcaaacaca gaggaaccgc tcggctgacg actggtaaag gcgtcggcat ggatagagac
    42301 aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc acttgcaatg gaatgaagat
    42361 gtcttcattg cgcgtcatgt acaccttcat attgccctct ttctcgatca gagagcgcaa
    42421 acggcgagct atttgcaata ccacatcttt ttcgcgcgtt ttgtatttcc ccaccgcacc
    42481 ggagtcttcg ccaccgtggc cagggtcaag cataatgaca atcggacgat cgcgccctgc
    42541 tttacccggt tgtggaccac tttgcgctgg cggcacctgc ttttcgaggt cgcctttgtt
    42601 gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc tgtgcattgg ccggatagag
    42661 gtccatcacc agacgctctt taaacccggc gaccggcgca agggcaaaca gctgcggttt
    42721 tacgttttgc tttaattcaa aaaccatccg tacggtttgc gggtcaaatt gcccgacgcg
    42781 cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc gccatcccct tgagtaccga
    42841 gttcaggttt acatcttcga tatccaccac cacgcgttca gggttactca acgcgaactg
    42901 cttatatttc agctgacgat ttgattccac cgtcacgcgg gtgtagctgg acgcaggcca
    42961 gacgcgcacc gccacgacct ggctgaccgc agccagactg acctgactta cgctcaatag
    43021 ccacatggca cccgcgcctt gcagtaaacg acgacggctg attgcagtgt tggatcctga
    43081 catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca agtaaacgac taatttgacc
    43141 ggaaacttta gcgaaagacg cataatctgt catctaataa acggtaaaca ttctttttat
    43201 attcacggca ttactgataa aaaagtcgct ctcgcataaa atttacactt gcaccctgcg
    43261 aaaaaacaga ataaaaatac actaatttcg aataatcatg caaagaggtg tgccgtggta
    43321 aaggaacgta aaaccgagtt ggtcgaggga ttccgccatt cggttcccta tatcaatacc
    43381 caccggggaa aaacgtttgt catcatgctc ggcggtgaag ccattgagca tgagaatttc
    43441 tccagtatcg ttaatgatat cgggttgttg cacagcctcg gcatccgtct ggtggtggtc
    43501 tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc atcaccacga accgctgtat
    43561 cacaagaata tacgtgtgac cgacgccaaa acactggaac tggtgaagca ggctgcggga
    43621 acattgcaac tggatattac tgctcgcctg tcgatgagtc tcaataacac gccgctgcag
    43681 ggcgcgcata tcaacgtcgt cagtggcaat tttattattg cccagccgct gggcgtcgat
    43741 gacggcgtgg attactgcca tagcgggcgt atccggcgga ttgatgaaga cgcgatccat
    43801 cgtcaactgg acagcggtgc aatagtgcta atggggccgg tcgctgtttc agtcactggc
    43861 gagagcttta acctgacctc ggaagagatt gccactcaac tggccatcaa actgaaagct
    43921 gaaaagatga ttggtttttg ctcttcccag ggcgtcacta atgacgacgg tgatattgtc
    43981 tccgaacttt tccctaacga agcgcaagcg cgggtagaag cccaggaaga gaaaggcgat
    44041 tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga aagcctgccg cagcggcgtg
    44101 cgtcgctgtc atttaatcag ttatcaggaa gatggcgcgc tgttgcaaga gttgttctca
    44161 cgcgacggta tcggtacgca gattgtgatg gaaagcgccg agcagattcg tcgcgcaaca
    44221 atcaacgata ttggcggtat tctggagttg attcgcccac tggagcagca aggtattctg
    44281 gtacgccgtt ctcgcgagca gctggagatg gaaatcgaca aattcaccat tattcagcgc
    44341 gataacacga ctattgcctg cgccgcgctc tatccgttcc cggaagagaa gattggggaa
    44401 atggcctgtg tggcagttca cccggattac cgcagttcat caaggggtga agttctgctg
    44461 gaacgcattg ccgctcaggc gaagcagagc ggcttaagca aattgtttgt gctgaccacg
    44521 cgcagtattc actggttcca ggaacgtgga tttaccccag tggatattga tttactgccc
    44581 gagagcaaaa agcagttgta caactaccag cgtaaatcca aagtgttgat ggcggattta
    44641 gggtaagcga tgaaaatcgt cggatgcgac atgcgtaaca ctcgtacgtc gcatccggca
    44701 attacgttta ttcccgtgaa ctaaacaacg ccgccagacc actgcgccgc tcagtacgag
    44761 tggcgattgc cgcacttaat atgcgctcat cggcatacag cgacagacgg cgacgcgcgc
    44821 gggtcaccgc ggtataaacc agctctcgcg ttactaccgg cgtgcgttgg ctcggcaaaa
    44881 tcaacgccgc atggtcgaac tccgatccct gcgatttatg taccgtcatc gcccacgtag
    44941 tttcgtgctc tggcaagcga ctcggttgca cagacttaat attgccgtcc ggcatcgcaa
    45001 accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc gatatcgcca ttaaacaacc
    45061 caagcgcgct gtcattacgg gcaatcatca ccggtcgacc ttcgtaccaa cgagagtgcg
    45121 gatgacgatg aattttgcgc ttctgttgca taaactgctc aattcgctca ttcagtccag
    45181 ccacgccaaa cggcccttcc cgcagggcgc acaaaagctg gtactcattg aacgcctgaa
    45241 tgattaaatc cggctcggca cgcgcttgca gcagatccag ataacgtccg taacccgcaa
    45301 gagcttcctc aagcatcgca atataatctt cgccgctctg taaaagccgt ttttcgatat
    45361 cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt atcaccacgg ttgatcgccg
    45421 cagctaactg accaatgcca gaatcgctgc cgaaacgata gcttttttgc agcaggcaga
    45481 gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc cggaacgtga gtccccgtca
    45541 ggcggcttag ctgcctggca cgctcggcgg taaagcccgc gttggcataa gcgcagatat
    45601 cgcccagcac agccccagcc tcaaccgagg ccagttgatc acgatcgccg agaaagatca
    45661 ctcgcgcatg atcgggcaag gcgtcgatca gtctcgacat cataggcaga tcgatcattg
    45721 acgcttcatc taccaccagc acatcaagat gcagcgggtt accggcatga tgacgtaaac
    45781 gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt gctggcatct tccggaatgc
    45841 gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc cttgccgaga gattcggtta
    45901 agcgcgcggc agctttaccc gttggtgcag ccagacggat acggcagcgt tcgccgtcgg
    45961 ccatttgaat taacgctgcc agcaacttcg ctacggtggt cgttttaccg gtgccagggc
    46021 cgccggaaat caccgagatc cgccgcgtca gcgccactgc cgccgcaact ttttgccagt
    46081 taatttcatc gcttactgga aaaagtttgt ccagggtttg cgccagtaga gcttcatcaa
    46141 cctcaatggc atgattcact tcgttgaaaa agcgtgccac tgtgcgctcg ttacaccaca
    46201 tgcgattcaa ataaagacga tcgccacaga ggatcatcgg cgtgggttca tctcccctgc
    46261 tgaccgcttg agaagccagc aagcattctt cccaattttg tagctcaccg atttcactga
    46321 cacaggtcgc caacagcgga tgcgacgcct cgttattttc cagtcgtgaa agcggcaaac
    46381 aaacgtgtcc ctctccggca tcatgactta acagtgccgc cgcgagggtg acggcaggat
    46441 gttcatctcc cgccacggtc agggcaaatt gcacatccag cgggcgtagc tgtttgtgct
    46501 ccacagcttc cagtaattgc ttttgcaatt tcattacgcc tcctccaggg tcataccggc
    46561 aaacatctca tccatcaggg caatcaaccc ggcgttgggt cgggttgtgt aaatcccctg
    46621 ttgcggatgt tctttatcaa cgccacgcag gaacagataa ataacgccgc caaagtggtg
    46681 ctcatagtcg taatcagcaa tgcgatggcg cagataacga tgcagcgcca gggtataaag
    46741 ctgatattgc agatcatagc ggtgtgcctg cattgccgct gccatagcct gttgggtgta
    46801 agccgaactg tcttcaccca accagttgga tttatagtcg agcaggtaat aacgcccttc
    46861 gtggcggaac accaggtcga taaagccttt taacatgcca cgtacctgca tgaactccag
    46921 cggcgggcag cctgcggata gcgggtcaaa ctggcggatt aacgtatcaa gctgactggc
    46981 gataagcggt tcactaatcg gcagataaaa ctccatctcc acctgtttat tgcgggcgga
    47041 aagttgactc aggcttacgc cggtttcatt gagaggtgcc tggaggacag ccgtgatcca
    47101 ctcggtcaat accggttccc actgcgattc aaagccgccg agttccagtt tttcccgcac
    47161 ccagttcggg tcaaccggct gggtaaaatc caggtcttca aacaaactgt gcaagaacgt
    47221 ccccggtgac gcaccgcgcg gaaactgatg tggtgttaac gtcggttctt caacgacgct
    47281 ggcaacgcct gcagcatcga catccagccg aggcatcaaa tcctgggcga taccgtgacc
    47341 acgctgttgc aaaccagagt agctggtgac gcgccagtta tcgccgggca atcgttgtaa
    47401 cgtcttcgca ttcagctctg ctgtagaaac atcattaacc tgccagggtt ggttatcacc
    47461 agtttgtgcc gtttgccagg caatatcatc atcgcataac gcttcaatac aggtgcgaag
    47521 ccctgccgca tcttgcggtt cccctttttg cagcaaacgc ccgagcgcac tttggtggac
    47581 gtcggtgtca ccttttttat cgccacgacg gcgcaccagc ggtgcaacgc cgagactgca
    47641 atgccaaacc gaacgtgtca gcgccacgta aagcaaacgc agatcttccg ccagacgttc
    47701 ggcctccgcg aggtcgacgc tttctggcgc agcattaaga tccagaactg cctcaaacga
    47761 gtggcgatcg tgataaaacg cctgctcctg gacgcggaaa ttggtgataa acggcagcca
    47821 gaccaatgga tattccagcc ctttcgattt gtggatcgtg acaatctgca ccagatgttt
    47881 atcactttcg agacgcattt gttggctgga ggcattactg tctggctcga ggatatgttg
    47941 cgataaccag cgtaccagcg catgttcact ttccagctgc gttccggctt cttgtagcag
    48001 ttcgctgata tgcaagatat cggtaagacg ccgctcaccg cctgccgttg ccagcaagtt
    48061 ttcagcaatg ttacgcgccg acatcagcgc ccgcagcatc ggcataacgc cacgtttgcg
    48121 ccagatttgc cgataaccat cgaactcttc gactaccaca tcccacgcat gttcgtcatt
    48181 gttcagcgtt tcgatatcca gcgcgttcag ccccatcatt gacgttgcca gcgcactacg
    48241 cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac aaccaaagca tttcctgcgc
    48301 ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa acggaaggga tttccagcaa
    48361 cgttaaggca tcgcgcacct gggcggcctc ctggcggctg cgcaccagca cactgatgtc
    48421 cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc aacgcttcgc cccgctgtcc
    48481 ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc gccatggtac tttgataatc
    48541 gccaacgccg cagctttcgc cttccatcag ccacattttc atcgcaggct gtgtttcacc
    48601 tttaaataca aaacgtaacg cctgattttt cccggctgat ttcactggaa taaacggtat
    48661 ttcgcgaaac atgaacgcgt catcagtctg gctgaaaagc ttattcacgc tgttcaccat
    48721 tcctggtgcg gaacgccagt tggtgtctaa agtgtagtgg gcgtgaactt cgctacgcgc
    48781 cttcatataa gtgaagatat ccgcaccccg gaatgcatat atggcctgct tcgggtcgcc
    48841 aattagcaac aatgcggttt ccggctgatg gtgccagata cggcgaaaaa ttcggtactg
    48901 ctgggggtcg gtatcctgaa attcatcgat cattgccacc gggaatcgcg tacggatcgc
    48961 cgctgccaac acctcaccgc tttcgctacg cagcgcggaa tcgagccgac ttaacatgtc
    49021 atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct actgtttcgc ggatctcagc
    49081 caatgcgcgg gtgatcacca gatcgcggat cgacaatggt tctgcaagca gttgatcgat
    49141 cgcctcaaac agtggatgtc gcggggtttc ccccccggcc ttcgtgcgat cttctaagaa
    49201 acgctgggag aatttttcca gcgactccgg caactgataa ctgtttgtct cttcttctgc
    49261 ccaggcgctg atcttgtcga tccatttagc ctgattgcta cggttaaact tgcgtcgatc
    49321 aataccagaa gattcgatca gcgcatccag ttcacccact gcgtcgcgcc actgctgttt
    49381 taccgtatca atacgcgcca caatttgcgc gtgacgggaa gccagcgttt catcatcggg
    49441 cggcggtgct ttgataaccg gcgcttcgcc ttgcagataa cgattaatat cgcgcagcaa
    49501 cgcctgcggc cctttccagg tttcaaagac gacctgggct atttcacgcg gcagcgggta
    49561 gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt agcagagact catcttcaat
    49621 cagctgctgc tcaaacagca tgccggattc aaaggcattc aggttgagca tgcgctggca
    49681 aaagccgtga atagtaaaga ctgccgcttc atccatctgc cgttcggcta acaacaacca
    49741 ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg cgttcgtaca gtggattgtc
    49801 ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata ttgctacgga tacgaccgcg
    49861 caattctgcc gtggcagcct cggtaaaggt gaccaccagc agttcttcaa cggtcagcgg
    49921 gcggggaaag gcggcggaac cgcctagtcc aagtaacagg cgcaaataga gcgccgcaat
    49981 cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg cgctcaccct gtaagggcaa
    50041 gcgcaaagga tctagtgtct cggcgacatc actcattctt ttcactcatc aggggcattg
    50101 tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc ttcagggtgt acatattcgg
    50161 ctttcccgtt ctggctgccg gaaatctgcg acagaatagc catgccttgc ggctcgacca
    50221 ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag cagttttatc tgggccacga
    50281 ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa atctttactt aacttcgatg
    50341 cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt aattaccgcc tgctggattt
    50401 gcgcaaactc atctggcttc atcgctcgca attttgcctc tgcggttggg aaaaacgcct
    50461 tgtaacgctc ccacaagaat gaaggctgtt tatcattgct ttgcaaaagg aagcccatgc
    50521 cccactgacg ccccacgctc attggaaacg caaacacggc atagcccaat tgttcttcgg
    50581 tacgcaactg attgtagaac cacggctgta cgatctgccc caacagagag ctataggctg
    50641 agctggtgta ttcatcgtag ccagtcggta caaataccgc tgccagtgcg gagtcggtgc
    50701 tgttaccggc tttttcaaag atgacggatt gttttttatc gaccactaca tctttgtttc
    50761 gacaccactc tgaaccatca gcgcccaact gtttttgcac atcgcgtgcc agcgttgttg
    50821 cctgggcctc ggtcatgttg ccgataacca taaactctgg tcgagcccct gattttaagg
    50881 cgtcgcgata ggccagcacc tctttcaacg taatggaggg caaaatttta cgccgttcat
    50941 ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg cataatcgcc tgctcaaacg
    51001 ctttaccctt ttctgcggaa tccatcatct ggttatacca ggacttcgcc tgctcaagct
    51061 gatcttccgt agcggtatag ctaaagtacc cctcgagcaa tgcctggaac agctgcggca
    51121 gacgctgggt gtaaccatta gcattaacca taaggccgtt gttagcgttg gtggaaaaac
    51181 ttatgccacc aaccgacgcc tggttgctta actgatcaag cgccagccct gcgagataat
    51241 cattgagcgc aaacatcacc tgattgcggg cgctgtccat ggctttcgga ttacgcaaaa
    51301 tcaggctgac atcagctttg ggctcgctgg caaaataacg gcttggcgca tacaccacgc
    51361 gcagattcga ctcatcaaca atcagctctg gatggtcgta tttcttctct gacttaatca
    51421 gcgagaaatc atcaggaata taagggttaa gctctggcaa agagagcgca atgtcggcgg
    51481 cttttttctg ccagtcggcg aaagtttgtg cgctgatttt atcgacctga tacggcgcat
    51541 cgacaaagta agccgttttg ttgtgcggct ctttcgggct gatataccag atacgcgcat
    51601 tctgcggcgt catcatcgcc agacgttcct ttactgcttt agcatcgtac cgatcggcaa
    51661 tattgactgc atccagcgta tgctcaacag gaacgcgaat catggtatct gccagccatt
    51721 cgacgtaatc catatcacgg gtgatcgacg gataacggaa gtcgatatcc agcacattcg
    51781 ccagttcatc gaagtattgt ttatcaatgc ctttttcacg taacagattg agatagctaa
    51841 aaattgccgc cacaacctga tcgcgattag ccaggccttt atcggttaaa gacgcagaga
    51901 tcgctaatac gccgctgttg ccgttgacga taggatcgga gttggcgcta atgccctcaa
    51961 ctaatccctg cttttgcagc cagtcagaaa gtgtacctgg gctgcgattg ccaatcagat
    52021 aggtaatcaa ttcatcggtt ttactacgga acttcgctga gttgttatcg atgcgaaact
    52081 caacgcgcaa cactttacgc ggcagcgcag ggacgtaatg aatgataatg cccttttgcg
    52141 cgtcggtgac taccggcacg gtgatttccg gttttttgct ctctttgttc ggcacgcgac
    52201 caaaggtgtc cgccgcaatt tttgccaact ccggcagcgg tttattactg taaataaccg
    52261 ccttcatcaa attggcggaa tagtacttct cgtggaaatc tttcagcgcc tgctgcaccg
    52321 gattaccagg tttgtcgctt aaagtttcga ggttaccacc agaaaacttt gaaccggggt
    52381 gtgccgggtt aatggtttct gcgctgacct gtgccatgcg catcccgtca cgcgtacgcg
    52441 ccatggttaa ttcagcgttc accgcattac gctcacgttc ggcatatttc ttgtcgagca
    52501 aaggttcagc aatagcatcg gccaggcggt ctaccgcacc aggcaaggcg tcgttctcaa
    52561 cttccagata gaaagccgtg cgatacggcg cagtgctggc attgtgacta ccgccgtgca
    52621 ttttgagata ttcggccaga ctgtcagcct gcgggtactt tttcgacccc atcagactca
    52681 tatgttcaag gtaatgtgcc agcccctggt acgcctcggg atcttccagc gacccaacgg
    52741 gcaccaccag cgccgagagc gatttaactg cctgcggatc agaaaccagc aagaccacca
    52801 taccgttatc cagacgtata gcctgatact ggcggttatc tttatcactt ttacggatgg
    52861 tttcctgaat cggctgccat cccgtttctg cctgacttaa gggtgcccaa agggcaacta
    52921 acaacaataa tgctttgaac caggtgctgc ggggcattca cggacctcat aatcaactta
    52981 attttctgtc cagattcaac acgttaacgc aaatcatctg ccagaattta atcttgtgct
    53041 gcacgagtca gcctatgttt atataaccat cagtccgtga ctggtgcgca tcataaagta
    53101 agcggataga ttgcgcaatt tttatacagc actcatgact gattaaagcg aaacagcggt
    53161 aacaggaaac gttgcgactg ttcaacgatg gcctccattg tctctggtgt taattgccgc
    53221 cagagccttt gataccagat atcatcacct tcgccacgca ccatcatgtt gccttcgtaa
    53281 gcctgaagga atttcgtacg ggctttttgc aacgtggaat cgtcatccag catggcatcg
    53341 ttttgcgcgt cataacaggt ttttagccac gcgccgccac tttcaggtaa caccagcaat
    53401 ggcgcggaca ttccttcacg atacccctca atcagttgtg agaggtaatg caaagcctgt
    53461 tcggctgcaa gcggcggaaa acgccactcg ccgtctttgc gtagaaaaag gcgactttca
    53521 ccattaccac cgctggcaca gtagacaagg tgttccagcc aaagttgcat tccctgggcc
    53581 acacttaata aagagggacg ccagcgcaac aggccatccg gctgcacctg cggcaaccag
    53641 ccagttatct gcacaccgtt gcaggcgaga tcaatttcca tactctgccc cggctggcga
    53701 caggcaatga ctctgtcggc aagctgctgc atctcctggc actgtgtttc ccagaaaatt
    53761 tcaccaaaag cgccatacgg taaatcccct gccgctcgga agcggcggaa caagcgttcg
    53821 gcatcatcct gctcaaccag tgcattcaat aactgctgat tgatttgata acggctaagt
    53881 ccttccagaa taaatggctc ggtgtcgggg atttcgctgt cttcagtacg gaagttcacc
    53941 tgcaaacgca tctggaaaaa tgcccgcacc ggatgtgccc agaatcgttg tagcgtttcc
    54001 agcggcacgg tttccggtaa ggtaaacggc agcggctgaa caaattcaga atgtgcttta
    54061 ccagcctggc tggccgcagg tagccattca cgagcatagc tttgtcgttc gcctggctgg
    54121 tagttttgtg gatcaaacgg catccgggta tggaggcaag taagatacgc ttttaccctt
    54181 gcctcgcttt catcacagtt gagcgcttca tcgcccggta gataatgact ttgcccgatg
    54241 tagtcgatca gttcctgcac cagtaccgac gggaaacgct cactgttatc ctgaatggaa
    54301 cgaccgatat agctgatata gagtttttgc tgcgcggaaa ttaacgcttc caggaacaga
    54361 tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg gtttctggct catcaggtca
    54421 aagcccaatg gcgcaagctg acgtggataa acgccgtcgt tcattcccag caggcaaacc
    54481 actttgaacg gaattgaacg cattggcatc agagtacaaa tgttaaccgg tccggcgaga
    54541 aaacgctggc tgatacgttc ttgatccaga cgctgtgcca gttcatcacg caatagtgac
    54601 agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg cgataatcgc ctgccattgt
    54661 tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg gcaggaagaa ggcgttgagc
    54721 atatcgcgac aaaccggcaa ccactcttcc agcggacgct cctgtgccag cccgcgacgc
    54781 cagatgttta gctgcattag cagtgaagcc agatgcccca ccagttctgc aattaagccg
    54841 ctcgattcat cataaggtag aaccgattgc cactcgccct gcgcgctctc catcgcgtag
    54901 cccaacaaca tacgcgtcag gccaaatcgc caggtgtgtt gtccggtggc ggggagttcc
    54961 agctcgcgaa cgttgtcgtc atctatgccc caacgaatgc cggattcgtt gacccactgg
    55021 cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg ccgccagcac cggcacatcc
    55081 agcaacgcca gcacatcctc tgacacaaaa cgactgtcag gcagtgataa caggctgata
    55141 aacgcttcca gtaccggatg tgactgccgc gcacgacggt cggaaatggc gtaaggtagg
    55201 taacgatccg caggtgcgct accaaacaca gcctgaataa acggactgta gctgtcgata
    55261 tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg ggtcttcctc cagcatcgcc
    55321 agcaggcgat cgtgtaaaac ttcaacttca cgctgcgggc tatggcaaac gtggaaggtg
    55381 atactgctat ccagtggatc aagcgggcgt ttgttatcgc tacgggaaaa ctcttcgatg
    55441 ttcacaccag caacggcgcg gttttccagt tccagaatgt cagactgaat gttatgcagc
    55501 aggttatctg gcgtgacatc gacaaaggcg tccagctcct ggctgctctc caggtcagaa
    55561 aggagataaa tgtagtcgcg cccaagctta ccccatgaag ccagcagcgg gttgccgaca
    55621 tcctgttcac catcgctgtt aaagagctgc ccggcatttt cgctatcacg aaatagcggc
    55681 aattcgcgat cttcaaaact gtgtcgacgc tgacgggtca gcagtttcgc cagataagca
    55741 ggatctttaa tatcgcccca gtaataacgg caagggttgg taaacaggag atggatttca
    55801 atatgtttac ccagcgcctg tagcgcctgg agataaacag gcggtaacgc ggaaataccg
    55861 catataaaga cgcgcgaagg taaccccggc gggcaggtcg tcgcggactc cagcgtttcg
    55921 ataaagcgct gatagagatt ggcgcggtgc cagcgcggtt gcccgagttg atgggtatat
    55981 tccaccagcg ccttccacaa cggggcttgc caggcctgtg cttctcccag cccttcaacc
    56041 aaatgtcctg tttcccactg tgccagccag tccggacgat agaccagata ctggtcaaac
    56101 aggtccgccg cttttgagga aagctggaac agttttcgct tgtcgctatc gtcagtcaga
    56161 taatgccgca acagggtaaa gtcttcgcgc tccagcaatt gcggcagcag agtcatcagt
    56221 ttccagctca tgctctgttt gttaaaggcg ctctctttgg ggatttccgg taacacccgg
    56281 acgaacatat cccagataaa gctcgctggc agcggaaaat caatgtttgc cgcaatacca
    56341 aacttttgcg acagggtcat ttgcagccac tgtgccatac cggtactttg caccagaatc
    56401 atctctggtt cgaaaggatc gtccagccgt tcgcgttcga caataaactc catcaacgct
    56461 tccagcacgt ccagacgatt ggaatggtag acccttaaca tagcggctcc tgactactga
    56521 cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc ccccgggtga aactagcgta
    56581 acgctgatgc tgacacatcc cgcctgcgat gtctgcattc ggttgacctg ccagttggca
    56641 ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccagccat gccgccagag ctgttggtac
    56701 tggtttctgc tggcaagact gttcattaat gttcgctgga aacccgataa cgcagtgaca
    56761 atcatcacca tcaacaccat cgccaacatt acctccggca ggctaaagcc ttgttgattc
    56821 ttcagggaag ctgacataac gccctctctt tcagcggaca aaaatcgctc cagccgcgtg
    56881 gcgaaaaaac aatgtttcca tcaatgactt cgcctgttcg ccacaacgaa acgccttcat
    56941 aaccggcaat caataaggct tcattatctg ccagtaaacg caaacaaacc tgggcatcgg
    57001 tttcagcgta ctgcgagcac tgaactgctg gctgcgtctg ccaggagtgc atttttcccc
    57061 acgccagcgc cgactgaacg atggcctggc ggcgcaatga ctgactttcc atgctcacgc
    57121 gagaggcaaa actgcgatcc tgttggctca ttccttgtaa tagcaaacta cccaaaacca
    57181 gcagcatcag gaccagagcc agtgacgaaa cacccttttc gcggttcaca ggttgaatcc
    57241 tgtcacgcta tagctggcat tcaccacggt ttgcggttca gacttactgg cagcacgcat
    57301 attaaccgtc aacaccggcg agaagccgct gacatcctga cgtacgacct gaaaagtgtc
    57361 gataatgatg gcatccggat tagtgacttt atcccagccc ttaccttcac aggatgtcgc
    57421 accgcgtagc gtttccagca catgctcctt cagacgaaat ccaatctggt cggactcttt
    57481 taccggttcg cgatcccaga taccgttact gttcgcatcc cactgcacaa tgacacagtc
    57541 accctgtccg acaatttcca gcccttcgcc ggtacagatg ccatgacaat aacccgccct
    57601 ctggagatgc ttcgcgacgg taaatacccg cagccagatt tcatcttcca gcgccagctt
    57661 acgggtgctc gttaaacttt cacgctgtaa cgcaggcaga aagcgtgccg cccccagcaa
    57721 caatacgcta ctgatcgcca tagcaatcaa cacttccagc agagaaaaac cttgctcttt
    57781 tacaggcatc cttctgtttc tccttgctga caaagccgga gtcttcccca cggcgaaacc
    57841 accagccacc actcgcccgt tgagtttttg aagcgaatat gcccggccca tgcggtattg
    57901 cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca tttcgacttc gggccagcgt
    57961 ggcacaaaga ccaatggtga actgccatga caggtattgg ccccagcagc ggaactcaca
    58021 aggcaccata acgtcccctc cctgataacg ctgatactgt ggtcgcggtt atgccagttg
    58081 gcatcttcac gtaaatagag caaatagtcc cgcgcctggc tggcggtttg ccatagccgt
    58141 tgcgactgct gccagtattg ccagccatag agtccacttg cgcttagcat gaccaaaatc
    58201 agcatcgcga ccagcgtttc aatcagcgta taaccacgtt gtgttttcat gccggcagta
    58261 tggagcgagg agaaaaaaag acgagggcca gtttctattt cttcggcgca tcttccggac
    58321 tatttacgcc gttgcaggac gttgcaaaat ttcgggaagg cgtctcgaag aatttaacgg
    58381 agggtaaaaa aaccgacgca cactggcgtc ggctctggca ggatgtttcg taattagata
    58441 gccaccggcg ctttaatgcc cggatgcgga tcgtagcctt caatctcaaa gtcttcgaaa
    58501 cggtagtcga agatggattc gggtttacgt ttgataatca acttcggcag cggacgcggt
    58561 tcgcggctta attgcagatg agtttgatcc atatggttgc tgtacagatg cgtgtcgcca
    58621 ccggtccaga caaaatcacc cacttccaga tcgcactgct gcgccatcat atgcaccaat
    58681 aacgcgtagc tggcaatgtt gaacggcagg ccgaggaaga cgtcacagga gcgctgataa
    58741 agctggcaag agagtttgcc gtctgccaca tagaactgga agaatgcatg gcacggtgcc
    58801 agcgccattt tatccagttc gcctacgttc cacgctgaaa caataatgcg gcgcgaatcc
    58861 gggtcgtttt tcagctggtt cagtaccgta gtgatctggt caatatgacg accatctggc
    58921 gttggccagg cgcgccactg tttaccatac actggcccga ggtcgccgtt ttcatcggcc
    58981 cattcgtccc agatggtgac attgttttcg tgtagataag caatgttagt gtcgccctgc
    59041 agaaaccaca gcagttcatg gatgatggaa cgcaggtggc aacgtttagt tgtcaccagc
    59101 gggaatccat cttgcaggtt aaaacgcatc tgatgaccaa aaatggaaag cgttccggtt
    59161 ccggtacggt cgtttttctg tgtgccttcg tcgagcactt tttgcatcag ttctaaatac
    59221 tgtttcatgg ttcctcagga aacgtgttgc tgtgggctgc gacgatatgc ccagaccatc
    59281 atgatcacac ccgcgacaat catcgggatg gaaagaattt gccccatgct gatgtactgc
    59341 acccaggcac cggtaaactg cgcgtcgggc tggcggaaaa actcaacaat gatgcgaaac
    59401 gcgccgtaac caatcaggaa caaacctgag acagctccca ttgggcgtgg tttacgaata
    59461 tacaggttga ggataataaa cagcaccaca ccttccagca gcagctcgta aagctgtgat
    59521 gggtggcgcg gcagcacacc gtaagtgtcg aaaatggatt gccactgcgg gttggtttgc
    59581 agcagcaaaa tatcttctgt acgggagcca gggaacagca tggcaaacgg gaagttcggg
    59641 tcaacgcggc cccacaattc accgttaata aagttgccca gacgcccggc accaagacca
    59701 aacggaatga gtggtgcgat aaaatcagag acctggaaga aggaacgttt agtacggcgg
    59761 gcgaagataa tcatcaccac gataacgcca atcaggccgc cgtggaaaga catgccgccg
    59821 tcccagacac ggaacagata cagcggatcg gccataaact gcgggaaatt gtagaacaga
    59881 acataaccaa tacgtccccc gaggaagacg ccgaggaagc ccgcatagag taagttttca
    59941 acttcatttt tggtccagcc gctgcccgga cgattcgccc gtcgtgttgc cagccacatt
    60001 gcaaaaatga aacccaccag atacatcagg ccgtaccagt gaagcgccac gggtcctatt
    60061 gagaaaatga ccggatcaaa ctccggaaaa tgcagatagc tactggtcat ctgtcaccac
    60121 aagttcttgt tatttcgctg aaagagaaca gcgattgaaa tgcgcgccgc aggtttcagg
    60181 cgctccaaag gtgcgaataa tagcacaagg ggacctagct ggttgccgga taccgttaaa
    60241 agatatgtat atgatccgcg ctataaccct ccgcgaatca gcccgcccat gccgcgacgc
    60301 tccataaagg ctgcaacctg atggcgaact tcggtcgcca gttgcgcttc cagactacgc
    60361 tgcgcaagat tttctgcttc ggcataatca atgcgccgca gcaggtattt tgcccgcgct
    60421 acagaacgtc cgttcataga cagatggcga tacccaagcc caatgaggat tgccacgcac
    60481 atgggatcgc ccgccatttc accgcacaaa cggagatcga ttccatgtat ttccgcttcc
    60541 cgggcgatca tcgccagagc tcgtaacatt gcaggatgaa gactgtcata aatgttcgcc
    60601 acccgggtat tgttgcgatc aacggccaga atgtattgag tcagatcgtt ggtgccaaca
    60661 gagatgaaat cgacccgctt tgccagatgc ggcagcataa ataccattga cggcacttcc
    60721 agcatgatgc cgatacgtgg tttgggaatt tcgtaaccga tcatctcctc gacttcacgt
    60781 ccggcacgtt caatcaggcg gcgtgcttca tcaacttcat cgaggcttgt gaccatcggc
    60841 aacagaatat tcaggttgcc cgtagcggca ttagcacgca gcatcgcccg cacctggatc
    60901 aagaagatct ccggctgatc gagcgtaatg cgaatcccac gccaacccag gcatggattc
    60961 tcttcgctga tcggcatgta aggcagctgc ttatctgctc cgacatccag cgtacgcaag
    61021 gtgacgggtt tatcattgaa catttgcagc atcccctgat actgcgccac ctgttcttct
    61081 tccgacggga aaccactttg cagcatgaat gggatttcag tgcgataaag accgatgcca
    61141 tcaatacggc tgcccagttt ttcttcatgt tccgggctta aaccagcatt gagcatgact
    61201 tttatacgct caccgctttt taactgcgcg ggtaaattga cgtcatcttc cgccagacgg
    61261 ctaagctcaa tctcttcact aattagccgc tgatattctt gcagcagtac cggctccgga
    61321 tcgaccagca attcaccgcg atagccatca acgatcagcg tcctacgatg cagcaccgaa
    61381 ggctgaatat ccgcgcccat cacggtaggg atccccagcg cacgtaccat gatcgcagca
    61441 tgggagttgg ctgcgccatc tcgcacgaca acaccgacta agcgatcctg gggcagctca
    61501 gcaagcgttg tcgctgacag ttcatctgcc accagaatga aacgttccgg ccaggcgttc
    61561 ggcccttgat tagcgtcatc aagatgaaac agcaatcgct gacccagcgc acgtaaatcg
    61621 ccagcccgct ctttgagata gttatcactt agcgcggcaa actgttcggc aaatttttca
    61681 atgaccgttt ttaccgccca ctctgccacc gagcctttat caacctcggc aaacaattcg
    61741 cgacgcagcc gggtgtccga aagcaggtgc gagtaaagat cgaaaatagc cgccgtttct
    61801 ttttgtgcac cggcggcaaa gcgtttgctg tagcggcgaa actcgtttgc cgcttcttcc
    61861 agcgccccgg tcagtcgttc gcgttccaga gccggatcca gcgttgatgc ctgatacacc
    61921 tgttccatta aaggtaacgt ggcatcctgc cagccttcgg caatcgccac accaggtgct
    61981 gccggtaatg cgcggattcg cgtctggcga tattgcccaa acaaggcagt caactgcgac
    62041 tgagaaagaa tagctgccat ctgggtggca agcgtcacca ggaaggattc ttcactttcg
    62101 tcatactggc gcaactctcg ttgctgtacc accagtacac caagcaactg gcgacgttga
    62161 ataattggta cgcctaaaaa cgcgcggaaa cgttcttctt ttacggaggg gatgtatttg
    62221 aagctggggt gcttttgcgc atctgcaagg tttatcggtt ccgccagcct gccaaccagg
    62281 ccgacgatcc cttcatcaaa cgcgagcgtt acagtgcgac cgcgtggttt tttcagcccc
    62341 cgggtcgcca tcaggtagta acaacgtcga tcatgatcgg ccaggtagac cgaacagacc
    62401 tcggtatcca tcgcaagaca gatgtcggta accagaatat ttaacgcctc attcaggcgt
    62461 ggtgcgctgg ctaccttttc gactatttcg cgcaggcgag tgagcataat tggcgtgact
    62521 taacctcttt tacgtcgata agcagatgcg ttttgtggtt ttggcgtatt ttcctgcagt
    62581 gacatcacca cactcgcgaa ctctttcatt accctacggt agacatcacg tttaaatgac
    62641 accacctgtc tgaccggata ccagtaactt acccatcgcc agccgtcaaa ctctggtgta
    62701 ctgctggttt gcatattgat ttctgcatcg ccgctcacca gctgcaagag aaaccatttt
    62761 tgtttttggc cgatacaaac cggcttcgtg tcccaacgca ccaaacgttt cggtaatttg
    62821 tagcgcaacc agttacgcgt tgaagcaagg attcgaacgt ctttgcggct taatcctact
    62881 tcttcaaaca attcacggta catcgcctgc tctgcggatt ctccggggtt gattccgcct
    62941 tgcggaaatt gccaggagtg ctgaccaaat cgccgggccc acattacctg cccctggcga
    63001 ttacaaatca cgatacctac gtttgggcgg tagccatcgt catcaatcac cggactacct
    63061 caaaataaag ctttatatac gaatgattgt ttcatactcc aggaagacgg taaaccactc
    63121 tctgcagggc attacacact aataacaatt gaataactca cagttatgtg cagagttata
    63181 aacagaggaa ggggtggata gccgttttta ttcacctttt ctgtggatag agttgtgaag
    63241 aactacggaa ttactacggg aaaacccgga gcattctgaa taagcccgat atagacattt
    63301 taaaatattc attatcagtc aatgcattac atgtaatttt agtaatgaaa aagagtaatt
    63361 cgtgacccag gtcacacctc tcatttacgg gttggcgaaa gatcaaccaa tgccgtattt
    63421 atccacagaa tgtgccacta agttaagcac tgaaccacta aaaactggag tttcgtcgca
    63481 cgtcaaggct gtaaatggaa acagtagtgg aggtttttca cagttatccc agctttctgt
    63541 ggataacatg gtgtaagatc ctgtttattt tcagtgacca gatttggaaa acccgttgca
    63601 gtgttgcgca actcgattac cggcaaccta aaaagcaata taaatcagag aattgaacaa
    63661 cgcatgtgga aaaagttaca ctgcgaatat tcggcacata attgctgttt gttttttaat
    63721 caaggtatca tgacatgtcc caacctcgcc cactgctctc tcctcccgaa actgaagaac
    63781 agttgttagc gcaagcacag caactttctg gttatacatt gggagaactg gcggcacttg
    63841 tcgggctggt tacgccagag aatttaaaac gcgataaagg ctggattggc gtgttactgg
    63901 agatctggct aggtgccagc gcagggagta aacctgagca agattttgct gctctgggcg
    63961 tggaacttaa aactatccct gtggatagtc ttggtcgtcc gctggaaaca acattcgttt
    64021 gtgttgcccc gttaacgggc aatagcgggg tgacctggga aaccagccac gtgcgccaca
    64081 aactcaaacg cgtactgtgg ataccggttg aaggcgagcg cagcatcccg ctggcgcagc
    64141 gtcgcgttgg atcaccgttg ctgtggagcc cgaatgaaga ggaagaccgg cagctacgcg
    64201 aagactggga agaattaatg gatatgattg ttctcggtca ggttgagcgg attaccgctc
    64261 gtcacgggga gtatttacag atacgaccga aagcagcgaa tgcgaaagcg cttaccgaag
    64321 ccattggtgc ccggggcgaa cggattctga cgctgccacg cggcttttat ttgaagaaga
    64381 atttcaccag tgcactactg gcccgtcatt ttctgatcca gtagccatcg ctttgacctg
    64441 ccgctttccg ggcatataat taccgcttca tttttttggc agggcttttt agatgttatt
    64501 tgcatggata accgatccta acgcctggct tgcgctcggt acgctgacgc tgctggagat
    64561 cgttcttggg atcgacaata ttattttcct ttctctggtg gtggcaaagc ttcccacagc
    64621 acaacgtgct catgcgcgcc gtctggggtt ggcgggagcc atggttatgc gtctggcgct
    64681 gctggcatca atcgcctggg ttacgcgcct gacaaatccg ctttttacaa tattcagtca
    64741 ggaaatttct gcccgtgatt tgattctgct tctgggtggc ttgttcctta tctggaaagc
    64801 cagcaaggaa atccacgaat ccattgaagg tgaagaagaa gggctgaaaa cacgcgtttc
    64861 atcattcctc ggcgctatcg tgcagattat gctgctggat attatcttta gcctcgactc
    64921 ggtgattacg gctgtgggtc tgtcagatca cctgtttatt atgatggcag ccgtggtgat
    64981 tgccgtaggc gtgatgatgt tcgccgcgcg ctcgattggt gattttgtcg aacgccatcc
    65041 ttcggtaaaa atgctggcgc tctctttcct gattctggtg ggctttaccc tgattctgga
    65101 aagtttcgac atccacgtac cgaaaggtta catctacttc gcgatgttct tctctattgc
    65161 ggttgaaagc ctcaacttga ttcgcaacaa aaagaatccg ctctgatact ccgtacgctc
    65221 tcctgcggga gggcgtactc actcctttta ccctcacagt ttaagatttt cctgctttca
    65281 agatatataa cgtcggttta taaacagact attatcatag gtaggccaaa tgaggataac
    65341 cggatgaaaa aatgggcagt aataatttct gcagtcggac tggcgtttgc tgtttccggg
    65401 tgttccagtg attacgtcat ggcgaccaaa gatggccgta tgattttgac cgatggaaaa
    65461 cctgaaattg atgatgatac cgggctggtg agttatcacg atcagcaagg taacgcgatg
    65521 caaattaacc gtgatgatgt ttcgcaaatt attgaacgtt aacaaataag gtcagcatcc
    65581 ggctggcctt aagatttttc tctttccctt ttcccttccc tctgccattt ttatattcct
    65641 tatgtcgtga ttataaaaag gaaacggcta tgcaatatca ccgtataccc cacagttcgc
    65701 tggaagtcag cacgctgggg cttggcacga tgacgtttgg tgaacagaac agcgaagccg
    65761 acgcccacgc acaactcgac tatgccgtcg ctcagggcat taaccttatc gacgttgccg
    65821 aaatgtaccc agtacctccg cgccccgaaa cgcaagggtt aaccgaaacc tacgtcggca
    65881 actggctggc gaaacatggc agccgcgaaa agttaattat cgcctccaaa gtgagcggac
    65941 cgtcgcgcaa taatgacaag ggcatccgcc cggatcaggc gctggatcgg aagaatatcc
    66001 gcgaagcgct gcatgacagc ctcaagcgcc tacagactga ttacctcgat ctttatcagg
    66061 tgcactggcc gcagcgcccg accaactgct tcggcaaact cggttatagc tggacggatt
    66121 ctgcgcctgc ggtttcgctg ctggatacgc tggacgcact ggcagagtac ctacgcgcgg
    66181 gaaaaattcg ttatatcggc gtgtcgaacg aaactgcatt tggcgtaatg cgctacctgc
    66241 atctggcgga caaacacgat ctgccgcgta ttgtcaccat tcagaaccct tacagtctgt
    66301 taaaccgcag ttttgaagta ggtctggcag aagtcagcca gtatgaaggg gtcgaactgc
    66361 tggcctattc gtgcctgggt ttcggcacgc tgaccgggaa atatctcaat ggtgcaaaac
    66421 ccgctggcgc acgtaatacg ctctttagtc ggttcacccg ctatagcggt gagcaaacgc
    66481 aaaaagccgt cgcggcgtat gttgatatcg ccagacgtca tggcctggac cctgctcaga
    66541 tggcgctcgc gtttgtacgc cgtcaaccgt ttgttgccag cactctgctg ggcgcaacca
    66601 cgatggatca gctgaaaact aacatcgaaa gtttgcatct ggagttaagc gaagacgtat
    66661 tagctgaaat tgaagcggtg catcaggttt atacttatcc ggcaccataa gattaacgcc
    66721 ggttaaaacc ggcgttaaat attaatgacg gcgctgccag atccacagcg ccgttattgc
    66781 cagcgcaaac agcgcaccga agccaatgcc aatgggcacg accgggatgc ctatcattac
    66841 cgccagcgag taaatgccca gcatcaacaa catggcgctg ttttcgccaa ggttttgtac
    66901 tgcaatcgca ttccccgccc cgacgctttt tttaccccgc tcctgtagca acgcattgag
    66961 cggaacgaca aaaaagcccc ccatcacgcc aatcagcatc aacaaggcat aggctggcag
    67021 cagctcgtgt tgcagggaaa aaatcagtac caccacgcca atcaaaatcc cggctggcat
    67081 acagcgtgac acggtttcca gcgtaactaa cttcgctgcc gcacctgcgc caaccacgat
    67141 accaatcgct accatcgcgt tgagataggt gggcgtagcg ttatcggtaa tgcccagcgc
    67201 caccggtacc cacagcacca acaggaaacg cagcgtgaca cccgctcccc agaataaact
    67261 ggtgcccacc agcgaaaaac gcgtttcacc attgcgccat agcgaggtgc aggcattcag
    67321 gaaactgcgg gtcatgttga tgagattcca ggactgcccc ggacgcgccg ccgccagttt
    67381 gggaatgtag atattggcaa cgaccgcacc accgtaggcc agtgcgcatg cggccagggc
    67441 gacgaggaca tgccagtcag ccagcacacc accggctacg gaaccgagca aaatcgccgc
    67501 tatggtagaa gcttccatta aaccgttagc tttcactaac ttactacccg tggttaattc
    67561 gccgagaata ccgtatttcg ccggtgaata ggctgcagca ccaacaccca ccagcgtata
    67621 gccgagaaac ggattgatac caaagcagat actggctgcg cccagcagct tcaggccgtt
    67681 ggcaaacatc atcacccggc ctttggcgaa gctatccgcc acctgcccga caaacggcgc
    67741 aaaaagaatg taagcaccta caaacaccat ttgcaggatg ggctggctcc actccggata
    67801 gaactgcgct ttcagtaacg ccagagtggc aaacagtagg gcattatcgc caaacgcaga
    67861 gagaaactgc gccacgataa ccgctttcat ccccttcgac cacaacgaag tgttagtgtg
    67921 cactgactca ctcatcgtgt tgttccgctt cgtctaccca gcttttcaac gtgacaaagt
    67981 caggtttgcc gctgccaagt aatggcatct gtttcagata gcgaatatcg cgcggtacag
    68041 caagctccgg cacgccgtgc tcgcgggcat actgttgcaa cttatcgcgc gtcagttcgt
    68101 tatctgtggt gaaaagcacc agtgcctcgc ctttgctggc atcgctctta atcgcagtgg
    68161 catggacttt atctggcgaa acaccaagtg ccagttgttc caccatttcc agcgacacca
    68221 tttcgcctgc aattttggca aagcgttttg cgcggccctg aatctgcaca aagccctgct
    68281 cgtcaaaacg cacaatatcg ccagtgtcat accagccgcg ctccatttcg ccgcgaacat
    68341 tctcggcggt gggcacttcc agtacacctg gcttctccac ccgcagatag ccgttcatta
    68401 tgttcggccc tttcagttgc aggcgtccgc cctcttcgat accagggacc gacaacaggc
    68461 gcgcatccat tcctggtaga atacgcccta ccgtaccggg tttcgccgcc atcggtacgt
    68521 tgatagaaac gacaggcgcg cattcggtca cgccgtagcc ttcaaggatg cgcaggccaa
    68581 atttatcctg ccaaagctgt ttggtacttt cttgtaattt ttctgcgcct gccaccacat
    68641 agcgtagacg atagaagtca tacgggttgg cgaaacgcgc gtagtgaccg aggaaagtcg
    68701 aggtgccgaa caacacggtg caactgcggt catacaccaa ctccggcaca atgcggtaat
    68761 gcagcgggct tggataaagg aacacttctg cacctgtaag cagtggcgta aacaggccta
    68821 ccgtcagccc aaaggagtga aacagcggta acgccgacat aaagcgatcg ttggtggtga
    68881 agtcggcaat cgttttaatc tgctcgacat tcgccagaat gcttttatgg ctatggacga
    68941 cgcctttcgg atggccttca gaaccggagg taaaaaggat cagcgcctct tcttccggct
    69001 gctgtttaac ctgtgccaga cgcggcatca gcaaatgagc gaagatccat actttgtcgg
    69061 cagtggtgac atctgctttt aaatcttcca gatagaccca gcgcacctga gtaagttgct
    69121 ccggcagatg ccagagtttg cctttatcga gaaactggcg ggaagtgaag atggttttga
    69181 tttcagccgc cgtaatagca ctggtcagcc cttttacccc ggcagtgtag ttcatcattg
    69241 cgggcatgcg gcgacgggcg atggccccaa aaatcactgc cgcactgatg cctgcattgg
    69301 gcagcattaa gccgatgcgt tcgccttcaa cactgtattt ttcaaggatg cgtccaacaa
    69361 acagcgtttt cgtaagcaat ttgcgatagg agtctggggt aaagttgacg tcttcgacac
    69421 atttcttccc ggctccgaag cggtacattg cactcagtaa agattcgtac agcgtttcac
    69481 gcgggcgcac cgccattcgc gcttccatca ttatttgatg cagcatttcg ccagcgattt
    69541 tgcgacggtc acgggcacgc ggcgcatccg gcatcgccac ctgcgttggt ggcaaaatat
    69601 gcagagtaat ttgcgggaac aagcgacgtt taaccagacc tttcaggcgg ctgaagtgcg
    69661 taagttccgc cccttcaata cgcacaggaa taaccgttgc accagacttc gccgcgacaa
    69721 aacccgcgcc atcgtagatt ttcatcagcg agcctgtcgt ggtgatgcgt ccttcaggga
    69781 aaatcaccac tggtcggccc tgttccacca gacgtaccag atgtttaata gccataggtt
    69841 gcgtcgggtc gagaggaaca aagtcgataa atgatttcag ccaacgcata taccactgtt
    69901 ggcttattga ggtgtaaacg gcaaacactg gacgcacagg taaaaacagt ccaagcaaaa
    69961 tgccatcaat aaaagagacg tgattaggcg taattagaac gcgctcgccc ttcagtgcct
    70021 gggtgtcacc cgtaacgcga acgcgataca aaacacggca caaatttcga aaaaagctaa
    70081 aaagcattcc aactcccttt gctctgattc agtaaaagcg aatggaggga gattacacga
    70141 gataaagaac gcgagcgaca gtaaattagg tgcgaaaaaa aacctgcgca tccgcgcagg
    70201 ttggtgcaag agacagggta cgaagagcgt accgaataat ctcaccaatc aatacctctg
    70261 ggatcttgat tgtggtctgc acgacgactc ttcgccagcg agaaaacgca aaggaatgaa
    70321 gggaaatgca acgaggtgtg taaattgtcg gttactgtta cagattgatg accggcaaaa
    70381 aaaacctgcg catctgcgca ggctggtgta attcatgtgc tcaacccgaa gttgacttca
    70441 cctatcaata cctctgggat caccacttta gcaacctgaa gccaaacgcc accagcggtc
    70501 aatcgcaaca gcgtttcgca aagtgtaacc aaaggtttgc aatctctttt tttgtcttac
    70561 ttatttgcgt ttggctcaca ttcccacgat gaaaacacgc caccccttga accaacgggc
    70621 gttttccgta acactgaaag aatgtaagcg tttacccact aaggtatttt catggcgacc
    70681 ataaaggatg tagcccgact ggcaggcgtt tcagtcgcca ccgtttcccg cgtcattaat
    70741 aattcaccca aagccagcga agcttcccgg ctggctgtgc atagtgcaat ggagtctctt
    70801 agctatcacc cgaacgccaa cgcccgtgcg ctggcgcagc agaccactga aacggtcggt
    70861 ctggtcgttg gtgatgtttc cgatccgttt ttcggtgcaa tggtgaaagc ggtcgaacag
    70921 gtggcttatc acaccggtaa ttttttattg attggcaacg gttaccacaa cgaacaaaaa
    70981 gagcgtcagg ccattgagca actgatccgc catcgctgtg ctgcgttggt cgtccatgcc
    71041 aaaatgatcc cggatgctga tttagcctca ttaatgaaac aaatgcccgg tatggtgctg
    71101 atcaaccgta tcctgcctgg ctttgaaaac cgttgtattg ctctggacga tcgttacggt
    71161 gcctggctgg caacgcgtca tttaattcag caaggtcata cccgcattgg ttatctgtgc
    71221 tctaaccact ctatttctga cgccgaagat cgtctgcaag ggtattacga tgcccttgct
    71281 gaaagtggta ttgcggccaa tgaccggctg gtgacatttg gcgaaccaga cgaaagcggc
    71341 ggcgaacagg caatgaccga gcttttggga cgaggaagaa atttcactgc ggtagcctgt
    71401 tataacgatt caatggcggc gggtgcgatg ggcgttctca atgataatgg tattgatgta
    71461 ccgggtgaga tttcgttaat tggctttgat gatgtgctgg tgtcacgcta tgtgcgtccg
    71521 cgcctgacca ccgtgcgtta cccaatcgtg acgatggcga cccaggctgc cgaactggct
    71581 ttggcgctgg cggataatcg ccctctcccg gaaatcacta atgtctttag tccgacgctg
    71641 gtacgtcgtc attcagtgtc aactccgtcg ctggaggcaa gtcatcatgc aaccagcgac
    71701 taaccgcagt taaagcaatt ccagcgccag taattcttcg atggtctggc gacggcgaat
    71761 caaccgcgcc tgaccattat caaacagaac ttctggtaac agcggacggc tattgtagtt
    71821 ggatgacatt gatgcgccat atgcccctgt atcatgcagt accagataat cacctgcctt
    71881 cacttccggc aaggcgcggg tttcaacatt tcccccttcc tgctgggtaa agacatcgcc
    71941 cgattcacat aacggtccgg cgacgacggt ttccaccgtt ggcgcgtgtt ccagagaacg
    72001 accatcagct gccagggcac tgatatggtg gtaactaccg tacattgccg ggcgcatcag
    72061 atcgttgaac ccggcatcaa ccagcacaaa gtggcggctc cccatttgtt tgacgctccg
    72121 cacctgagta attaatacgc cagactgcgc taccaggaag cgacccggtt caatttccag
    72181 tttcacaggg tggcccaaat ggcgggcgat ttgctcacgc gcggcattcc acagaccata
    72241 ataatgttcg gtatcaaccg cctcttcacc ctgttgataa ggaacagaaa gcccaccgcc
    72301 cgcagaaata gcctgtaaat cctgaccgaa ttcgatgacc tgacgcacca tagcaccaca
    72361 cacctgttcc agatgggcat aatcaacgcc agaaccaatg tgcatgtgaa tgccgaccag
    72421 ctgcagatga tgacgttgta tcacgtccag tgcggcgggc agatcggtgt accagatacc
    72481 gtgcttgctg ttttcgccac cggtattggt tttttggcta tgtccgtgac caaaccccgg
    72541 attaacgcgc agccataccc gatgccctgg cgaaacctgg cccagttggt cgagcatatc
    72601 aacagaaccc gcattcaccg gaatttgcaa ttcactgacg cgttcaagcg tcgcctgatc
    72661 gataacatct gccgtaaaaa caatatcatc ggggtgcgtt tgcggattgt aacccgccgc
    72721 caacgcacgc tctatttcgc ctaacgagac ggaatccact ttcacgccct gctcacgcat
    72781 taagcgcaaa atatgaatat tggaacaggc tttctgtgca aagcgcacca catcaaactg
    72841 tttcagcgct gcaatctgcc gacgaataat ttgcgcatcg tagacccaca ccgggcagcc
    72901 aaattcagcg ggcaaacgca gcagattttc ggcggtgaga tcggtatcgg tgctgaacag
    72961 tgaatgtggc ataacaaact ccagataagt gcttttttat gattacgcca catcataaaa
    73021 agaataaaaa atatcgattt atgtcgagtc tatgcaaaaa tgatatggat taccggattg
    73081 cgagagagcg ctaatggccg ccgttaactt acgtcatatt gaaatttttc atgcggtaat
    73141 gaccgccgga agcctgactg aggcggcaca cctgctacac acctcacagc caaccgtcag
    73201 ccgcgaactt gcgcgctttg agaaggtgat cgggctgaaa ttgtttgagc gcgtacgtgg
    73261 gcgattacat cctaccgtgc aaggactgcg tctgtttgaa gaagtgcaac gatcctggta
    73321 cggactggat cgcattgtca gcgccgcaga aagtctgcgc gagtttcgcc agggagaact
    73381 gtctattgcc tgcctgccgg tcttttcgca atctttttta ccgcagctcc tgcaaccctt
    73441 tctggcacgt tatcccgatg tcagcttaaa tatcgtgccc caggaatcac cgctacttga
    73501 agagtggctc tcggcccagc gtcatgattt aggactcact gaaacgctcc atacgcctgc
    73561 gggaacagaa cgtaccgaat tactctcttt agatgaagtg tgtgtgttac ctccgggtca
    73621 tccgctggcg gtaaaaaagg tattaacgcc ggatgatttt cagggtgaga actacatcag
    73681 cctttcccgt actgacagct atcgccagtt gctggatcag ctatttactg aacatcaggt
    73741 taaacgacgc atgatcgtag aaacccacag cgccgcgtca gtctgcgcaa tggtacgggc
    73801 gggggtaggt gtttcggtgg ttaacccgct caccgcacta gattatgcgg caagcggttt
    73861 agtggtgcgg cggttcagta ttgcggttcc gttcaccgtc agcctgatcc gccccctgca
    73921 ccgcccgtca tcagcgctgg ttcaggcgtt tagtgggcat ttacaagcgg ggttaccgaa
    73981 actggtcact tctcttgacg ctattttgtc gtcagctacg acagcataaa agcgacagca
    74041 tcctcggcat ggatcgccgc ggtatcaaac acaggcaaaa cactgcgctc ttctggcacc
    74101 agtaaaccaa tttctgtgca gccaaaaatg acgccctgtg cgccctgttc tgcaaggcga
    74161 gcaatcactt gcgcataata agcgcgtgac gcttcggtaa attgccccag acacagttct
    74221 tcaaaaataa tctgattaat tttcgcccgt tcatccgctt caggaataag acaattgatg
    74281 gaaaattgtt ccgtcagccg cccgcgataa aaatcctgtt ccatggtgta acgcgtaccc
    74341 agcagcgcca cacgagtcat tccggccccg gtaattgcac gtccggtggc atccgcaatg
    74401 tgtaagaaag gcagagtgca acgtgactca atggcatccg ccactttatg catcgtattg
    74461 gtacataaca caataccttc tgcgcccgcc cgctgtaagc caagcgccgc ctcagccaga
    74521 atgtccccgg ttttatccca ttccccgcga cgctggcact cttctatttc atgaaaatcg
    74581 acgctatgta gcagcacttg cgcagagtga agcccaccaa gccgctgttt aatgccttca
    74641 tttatcaaac gatagtaagg aatggtggat tcccagctca ttcctcccag caaaccaatt
    74701 gttttcattc ctctctcctg atgtgtgtta ccgcaacaag gctgaataag gaacgagaca
    74761 aacgcctcaa cggccaagtg cccaatctct attaacgaaa aaagggccgg atgtacagca
    74821 catccggccc gtgaaatcag acgccgatat ttctcaactt ctcgcctgcc atcagtttgc
    74881 gttcgatatg ttccagcgtg acatttttgg tttccggaat gagccagaaa gtaatgccca
    74941 caaacgcaat gttcagcgca gtgtagagcc agaacgtacc ggcagcgcca atgctatcaa
    75001 gcagtgtcag gaaggtcgcg ccgataatca tattcgacac ccagttcgtg gtggtcgaac
    75061 aggtaatacc gaaatcgcgg catttcagcg gctgaatttc agagcacagg atccacacca
    75121 ctggcgcggc gctcatcgca taaccggcaa tacacatcat cgtcatgcca acagagagcc
    75181 aggacaagcc actggaagcc gtaccgttat caaactgcat caggcaatag cccagcacca
    75241 gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc cggtttacgc cctgctttat
    75301 ctaccgtaaa caccgcaata aaggtggcga acataaaggt cagccctacg accagagtcg
    75361 caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc ggcgcgtagt
    75421 acatgatgat gttcataccg gtaaactgct gcatcgcctg caacaacata ccgagaaaca
    75481 cagcacgacg gacgttacgg ttgatcttaa acagtgccca accgccctgt tttaacttca
    75541 ggctttcacg aatttcgttg agttcttctc gcgctttttc cgacgtatcg cgcagcatac
    75601 gcaatacttc ttccgcctca atatgacgcc ccttttccgc cagccagcgc gggctatttg
    75661 gcaggaagac taccagaata atcagcagaa ctgctggtaa agcaagaacc cccaacattg
    75721 cgcgccagtt accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga
    75781 gtgtgaccat caactggtac atactgatca tcttaccgcg aacgttttca cttgccattt
    75841 cagaaagata cagaggagcg gtgtaagacg cgatcccgac agcaatgccc agcaccacac
    75901 gagcggcgat taacatctct acgctggtcg caaaagcgga ccctatagaa ccgagtacaa
    75961 acaggatggc ccccgccatc aggctgtatt tacgccccag gcggaacgac agccaaccat
    76021 taaacagcgc accaattgct gcaccgagca tcatgctact aaccacccat tcctgcaaac
    76081 gactggtcag cacaaagtga tcggtaatga acggcaacgc tccggcgatt acgccgatat
    76141 caagaccaaa taacaatcct gcgaccgcag cagctaccga aacaaacata ttcatacgcc
    76201 gcgtatcccg caaagaacgt ggcgttaaag cagattccgt attgatagta accatttttt
    76261 cctgccagca gagagtaaga catagtgaaa aaatacgtga acaactcacg caggtgtcag
    76321 gtcggaaaca gcataaatat ggattaaat
//
LOCUS       DBBGLQ010000016        75635 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.016, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000016 DBBGLQ010000000
VERSION     DBBGLQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.016"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            162..584
                     /gene="yfbO"
                     /locus_tag="ACIYSB_001368"
     CDS             162..584
                     /gene="yfbO"
                     /locus_tag="ACIYSB_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfbO"
                     /protein_id="HGC5428448.1"
                     /translation="MTPLERITQLVNINGDVNNPDTPRPLLSLEDFFIDNNIHGSICC
                     NVIPEQSPQAIYHHFLKIRERNNVSDVLVEITMFDDPDWPFSESILVITTASPEEVQS
                     WFVEEIAPDECWEGWSEDTEHGWVEVPVGMHPVTCWWD"
     gene            643..1491
                     /gene="yfbP"
                     /locus_tag="ACIYSB_001369"
     CDS             643..1491
                     /gene="yfbP"
                     /locus_tag="ACIYSB_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein YfbP"
                     /protein_id="HGC5428449.1"
                     /translation="MKLIPRSSDISPGIDGICPGPFPPNGFTVLTDAAYGNGDCFGLY
                     WPIGQEHKLPIVCETYHDEWRIVPAFSSIKKFEEWLEVNDDDPHENGISIEDQDFAAN
                     LFRVARKCLSTGRLDDALPLLQRATEQLPEVSEYWLALAIQYRRCKKTEAAAQAALNA
                     YLGNWAFGVPDNKVIHLLSQAADVPNFQDDPVIQCIKEQGLDLSFGGTKENNNYPLMQ
                     MCVDTYFAQRKPLQALTLLHNYAWIMSSETTAFQERYDFNIDEWRAKFRQLCLEYFGD
                     SRTQFT"
     gene            complement(1575..3032)
                     /gene="nuoN"
                     /locus_tag="ACIYSB_001370"
     CDS             complement(1575..3032)
                     /gene="nuoN"
                     /locus_tag="ACIYSB_001370"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGC5428450.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3039..4568)
                     /gene="nuoM"
                     /locus_tag="ACIYSB_001371"
     CDS             complement(3039..4568)
                     /gene="nuoM"
                     /locus_tag="ACIYSB_001371"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGC5428451.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4732..6573)
                     /gene="nuoL"
                     /locus_tag="ACIYSB_001372"
     CDS             complement(4732..6573)
                     /gene="nuoL"
                     /locus_tag="ACIYSB_001372"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGC5428452.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQTYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6570..6872)
                     /gene="nuoK"
                     /locus_tag="ACIYSB_001373"
     CDS             complement(6570..6872)
                     /gene="nuoK"
                     /locus_tag="ACIYSB_001373"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGC5428453.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6869..7423)
                     /gene="nuoJ"
                     /locus_tag="ACIYSB_001374"
     CDS             complement(6869..7423)
                     /gene="nuoJ"
                     /locus_tag="ACIYSB_001374"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGC5428454.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7435..7977)
                     /gene="nuoI"
                     /locus_tag="ACIYSB_001375"
     CDS             complement(7435..7977)
                     /gene="nuoI"
                     /locus_tag="ACIYSB_001375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGC5428455.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7992..8969)
                     /gene="nuoH"
                     /locus_tag="ACIYSB_001376"
     CDS             complement(7992..8969)
                     /gene="nuoH"
                     /locus_tag="ACIYSB_001376"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGC5428456.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8966..11692)
                     /gene="nuoG"
                     /locus_tag="ACIYSB_001377"
     CDS             complement(8966..11692)
                     /gene="nuoG"
                     /locus_tag="ACIYSB_001377"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGC5428457.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASAIRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11745..13082)
                     /gene="nuoF"
                     /locus_tag="ACIYSB_001378"
     CDS             complement(11745..13082)
                     /gene="nuoF"
                     /locus_tag="ACIYSB_001378"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGC5428458.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13079..13579)
                     /gene="nuoE"
                     /locus_tag="ACIYSB_001379"
     CDS             complement(13079..13579)
                     /gene="nuoE"
                     /locus_tag="ACIYSB_001379"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGC5428459.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13582..15384)
                     /gene="nuoC"
                     /locus_tag="ACIYSB_001380"
     CDS             complement(13582..15384)
                     /gene="nuoC"
                     /locus_tag="ACIYSB_001380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGC5428460.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRVRTPSF
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15478..16140)
                     /gene="nuoB"
                     /locus_tag="ACIYSB_001381"
     CDS             complement(15478..16140)
                     /gene="nuoB"
                     /locus_tag="ACIYSB_001381"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGC5428461.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16156..16599)
                     /gene="nuoA"
                     /locus_tag="ACIYSB_001382"
     CDS             complement(16156..16599)
                     /gene="nuoA"
                     /locus_tag="ACIYSB_001382"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGC5428462.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17230..18168)
                     /gene="lrhA"
                     /locus_tag="ACIYSB_001383"
     CDS             complement(17230..18168)
                     /gene="lrhA"
                     /locus_tag="ACIYSB_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGC5428463.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYILQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19088..20305
                     /gene="alaA"
                     /locus_tag="ACIYSB_001384"
     CDS             19088..20305
                     /gene="alaA"
                     /locus_tag="ACIYSB_001384"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGC5428464.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20389..20988
                     /gene="yfbR"
                     /locus_tag="ACIYSB_001385"
     CDS             20389..20988
                     /gene="yfbR"
                     /locus_tag="ACIYSB_001385"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGC5428465.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEIFVPSFHLSLDEISQDSPL"
     gene            complement(21047..22879)
                     /gene="yfbS"
                     /locus_tag="ACIYSB_001386"
     CDS             complement(21047..22879)
                     /gene="yfbS"
                     /locus_tag="ACIYSB_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGC5428466.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22966..23616)
                     /gene="hxpA"
                     /locus_tag="ACIYSB_001387"
     CDS             complement(22966..23616)
                     /gene="hxpA"
                     /locus_tag="ACIYSB_001387"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGC5428467.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23627..24121)
                     /gene="yfbU"
                     /locus_tag="ACIYSB_001388"
     CDS             complement(23627..24121)
                     /gene="yfbU"
                     /locus_tag="ACIYSB_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGC5428468.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24204..24659)
                     /gene="yfbV"
                     /locus_tag="ACIYSB_001389"
     CDS             complement(24204..24659)
                     /gene="yfbV"
                     /locus_tag="ACIYSB_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGC5428469.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24997..26199
                     /gene="ackA"
                     /locus_tag="ACIYSB_001390"
     CDS             24997..26199
                     /gene="ackA"
                     /locus_tag="ACIYSB_001390"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGC5428470.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26274..28418
                     /gene="pta"
                     /locus_tag="ACIYSB_001391"
     CDS             26274..28418
                     /gene="pta"
                     /locus_tag="ACIYSB_001391"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGC5428471.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28608..30128
                     /gene="yfcC"
                     /locus_tag="ACIYSB_001392"
     CDS             28608..30128
                     /gene="yfcC"
                     /locus_tag="ACIYSB_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGC5428472.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30161..30703)
                     /gene="yfcD"
                     /locus_tag="ACIYSB_001393"
     CDS             complement(30161..30703)
                     /gene="yfcD"
                     /locus_tag="ACIYSB_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGC5428473.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30761..31312)
                     /gene="yfcE"
                     /locus_tag="ACIYSB_001394"
     CDS             complement(30761..31312)
                     /gene="yfcE"
                     /locus_tag="ACIYSB_001394"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGC5428474.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31368..32012)
                     /gene="yfcF"
                     /locus_tag="ACIYSB_001395"
     CDS             complement(31368..32012)
                     /gene="yfcF"
                     /locus_tag="ACIYSB_001395"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGC5428475.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32148..32795
                     /gene="yfcG"
                     /locus_tag="ACIYSB_001396"
     CDS             32148..32795
                     /gene="yfcG"
                     /locus_tag="ACIYSB_001396"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGC5428476.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32852..33214
                     /gene="folX"
                     /locus_tag="ACIYSB_001397"
     CDS             32852..33214
                     /gene="folX"
                     /locus_tag="ACIYSB_001397"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGC5428477.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33235..34128
                     /gene="yfcH"
                     /locus_tag="ACIYSB_001398"
     CDS             33235..34128
                     /gene="yfcH"
                     /locus_tag="ACIYSB_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGC5428478.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34176..35066)
                     /gene="rpnB"
                     /locus_tag="ACIYSB_001399"
     CDS             complement(34176..35066)
                     /gene="rpnB"
                     /locus_tag="ACIYSB_001399"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGC5428479.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVLPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35263..36036)
                     /gene="hisP"
                     /locus_tag="ACIYSB_001400"
     CDS             complement(35263..36036)
                     /gene="hisP"
                     /locus_tag="ACIYSB_001400"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGC5428480.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQRFLKGSLK"
     gene            complement(36044..36760)
                     /gene="hisM"
                     /locus_tag="ACIYSB_001401"
     CDS             complement(36044..36760)
                     /gene="hisM"
                     /locus_tag="ACIYSB_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5428481.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVSHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36757..37443)
                     /gene="hisQ"
                     /locus_tag="ACIYSB_001402"
     CDS             complement(36757..37443)
                     /gene="hisQ"
                     /locus_tag="ACIYSB_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGC5428482.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPSMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37533..38315)
                     /gene="hisJ"
                     /locus_tag="ACIYSB_001403"
     CDS             complement(37533..38315)
                     /gene="hisJ"
                     /locus_tag="ACIYSB_001403"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGC5428483.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38536..39318)
                     /gene="argT"
                     /locus_tag="ACIYSB_001404"
     CDS             complement(38536..39318)
                     /gene="argT"
                     /locus_tag="ACIYSB_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGC5428484.1"
                     /translation="MKKSILALSLLVGLSTAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39584..40153)
                     /gene="ubiX"
                     /locus_tag="ACIYSB_001405"
     CDS             complement(39584..40153)
                     /gene="ubiX"
                     /locus_tag="ACIYSB_001405"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGC5428485.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40248..41765)
                     /gene="purF"
                     /locus_tag="ACIYSB_001406"
     CDS             complement(40248..41765)
                     /gene="purF"
                     /locus_tag="ACIYSB_001406"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGC5428486.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41802..42290)
                     /gene="cvpA"
                     /locus_tag="ACIYSB_001407"
     CDS             complement(41802..42290)
                     /gene="cvpA"
                     /locus_tag="ACIYSB_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGC5428487.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42549..43211)
                     /gene="dedD"
                     /locus_tag="ACIYSB_001408"
     CDS             complement(42549..43211)
                     /gene="dedD"
                     /locus_tag="ACIYSB_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGC5428488.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43201..44469)
                     /gene="folC"
                     /locus_tag="ACIYSB_001409"
     CDS             complement(43201..44469)
                     /gene="folC"
                     /locus_tag="ACIYSB_001409"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGC5428489.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSLVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44539..45453)
                     /gene="accD"
                     /locus_tag="ACIYSB_001410"
     CDS             complement(44539..45453)
                     /gene="accD"
                     /locus_tag="ACIYSB_001410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGC5428490.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45609..46268)
                     /gene="dedA"
                     /locus_tag="ACIYSB_001411"
     CDS             complement(45609..46268)
                     /gene="dedA"
                     /locus_tag="ACIYSB_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGC5428491.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPLLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46351..47163)
                     /gene="truA"
                     /locus_tag="ACIYSB_001412"
     CDS             complement(46351..47163)
                     /gene="truA"
                     /locus_tag="ACIYSB_001412"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGC5428492.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTALRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47163..48176)
                     /gene="usg"
                     /locus_tag="ACIYSB_001413"
     CDS             complement(47163..48176)
                     /gene="usg"
                     /locus_tag="ACIYSB_001413"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGC5428493.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48242..49378)
                     /gene="pdxB"
                     /locus_tag="ACIYSB_001414"
     CDS             complement(48242..49378)
                     /gene="pdxB"
                     /locus_tag="ACIYSB_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGC5428494.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLYDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49477..50472
                     /gene="flk"
                     /locus_tag="ACIYSB_001415"
     CDS             49477..50472
                     /gene="flk"
                     /locus_tag="ACIYSB_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGC5428495.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGTGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50469..51647)
                     /gene="yfcJ"
                     /locus_tag="ACIYSB_001416"
     CDS             complement(50469..51647)
                     /gene="yfcJ"
                     /locus_tag="ACIYSB_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5428496.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTVLSFRRG
                     "
     gene            complement(51912..53132)
                     /gene="fabB"
                     /locus_tag="ACIYSB_001417"
     CDS             complement(51912..53132)
                     /gene="fabB"
                     /locus_tag="ACIYSB_001417"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGC5428497.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53291..55297
                     /gene="mnmC"
                     /locus_tag="ACIYSB_001418"
     CDS             53291..55297
                     /gene="mnmC"
                     /locus_tag="ACIYSB_001418"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGC5428498.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55418..55696)
                     /gene="yfcL"
                     /locus_tag="ACIYSB_001419"
     CDS             complement(55418..55696)
                     /gene="yfcL"
                     /locus_tag="ACIYSB_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGC5428499.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55730..56278)
                     /gene="epmC"
                     /locus_tag="ACIYSB_001420"
     CDS             complement(55730..56278)
                     /gene="epmC"
                     /locus_tag="ACIYSB_001420"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGC5428500.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56278..57087)
                     /gene="yfcA"
                     /locus_tag="ACIYSB_001421"
     CDS             complement(56278..57087)
                     /gene="yfcA"
                     /locus_tag="ACIYSB_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGC5428501.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57087..57911)
                     /gene="mepA"
                     /locus_tag="ACIYSB_001422"
     CDS             complement(57087..57911)
                     /gene="mepA"
                     /locus_tag="ACIYSB_001422"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGC5428502.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57915..59000)
                     /gene="aroC"
                     /locus_tag="ACIYSB_001423"
     CDS             complement(57915..59000)
                     /gene="aroC"
                     /locus_tag="ACIYSB_001423"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGC5428503.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLDIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59035..59967)
                     /gene="prmB"
                     /locus_tag="ACIYSB_001424"
     CDS             complement(59035..59967)
                     /gene="prmB"
                     /locus_tag="ACIYSB_001424"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGC5428504.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60133..60684
                     /gene="smrB"
                     /locus_tag="ACIYSB_001425"
     CDS             60133..60684
                     /gene="smrB"
                     /locus_tag="ACIYSB_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGC5428505.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60756..61613)
                     /locus_tag="ACIYSB_001426"
     CDS             complement(60756..61613)
                     /locus_tag="ACIYSB_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGC5428506.1"
                     /translation="MKILRYVFYFLLLICLSTGVQAAKKIATLYIPAGSMGVLTGFQF
                     NLSILTPEGAQYGVYQSRSVAILNVPLVSWTGQGTAPELHLEDARTILPTSRCPGLEE
                     YDARTPQTTWACVEMKVTVFYDGDMHGCPWIVSSYVKSEDATEAIYTGNNVPYIGPTK
                     VSSDCSAIPLDPYDVSWNENTVVHNKIVRMQSTGAVIEQTLPTYLMEGGKLCDGSKYD
                     ERGAYCRFVAQQMTFSTSGCDDAKVTVTPEPQPITSRQLHDMKLRVDTTAQQPIDATC
                     RFTYILNMY"
     gene            complement(61615..62154)
                     /locus_tag="ACIYSB_001427"
     CDS             complement(61615..62154)
                     /locus_tag="ACIYSB_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5428507.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLIVAGQSVHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGEFTASLTATFEYI"
     gene            complement(62151..62639)
                     /locus_tag="ACIYSB_001428"
     CDS             complement(62151..62639)
                     /locus_tag="ACIYSB_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5428508.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQEVDYELSCESDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTLSAYATLRVD
                     YQ"
     gene            complement(62636..63145)
                     /locus_tag="ACIYSB_001429"
     CDS             complement(62636..63145)
                     /locus_tag="ACIYSB_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5428509.1"
                     /translation="MSHKFLAGAGLLVLAGWGVSGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVEFGNVVINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFSVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(63161..63913)
                     /locus_tag="ACIYSB_001430"
     CDS             complement(63161..63913)
                     /locus_tag="ACIYSB_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGC5428510.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNNCQVDEEQSRKG"
     gene            complement(63933..66578)
                     /gene="yfcU"
                     /locus_tag="ACIYSB_001431"
     CDS             complement(63933..66578)
                     /gene="yfcU"
                     /locus_tag="ACIYSB_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HGC5428511.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDVSKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDGKCLKPGQLEGVEIKADLSQSALVISLPQAYLEYTWPDWD
                     PPSRWDDGISGIIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFGGDDTQKKWEWSRYYAWRALPSLKAKLALGEDYLNSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGGFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNMSVSLTGY
                     RYEYDNRADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNIG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTTHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVTSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(66660..67223)
                     /gene="yfcV"
                     /locus_tag="ACIYSB_001432"
     CDS             complement(66660..67223)
                     /gene="yfcV"
                     /locus_tag="ACIYSB_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5428512.1"
                     /translation="MSKFVKTAIAAAMVMGAFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKNFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTATNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(67904..68389)
                     /gene="sixA"
                     /locus_tag="ACIYSB_001433"
     CDS             complement(67904..68389)
                     /gene="sixA"
                     /locus_tag="ACIYSB_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGC5428513.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68592..70736)
                     /gene="fadJ"
                     /locus_tag="ACIYSB_001434"
     CDS             complement(68592..70736)
                     /gene="fadJ"
                     /locus_tag="ACIYSB_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGC5428514.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     QVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTQGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRISAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(70736..72046)
                     /gene="fadI"
                     /locus_tag="ACIYSB_001435"
     CDS             complement(70736..72046)
                     /gene="fadI"
                     /locus_tag="ACIYSB_001435"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGC5428515.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72227..72511)
                     /gene="yfcZ"
                     /locus_tag="ACIYSB_001436"
     CDS             complement(72227..72511)
                     /gene="yfcZ"
                     /locus_tag="ACIYSB_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGC5428516.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            72883..74223
                     /gene="fadL"
                     /locus_tag="ACIYSB_001437"
     CDS             72883..74223
                     /gene="fadL"
                     /locus_tag="ACIYSB_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGC5428517.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            74589..>75635
                     /gene="yfdF"
                     /locus_tag="ACIYSB_001438"
     CDS             74589..>75635
                     /gene="yfdF"
                     /locus_tag="ACIYSB_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HGC5428518.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEVNGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIDSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNDVFKRYGRA
                     IRYDFQVDDAKYKCDHLKEIVSTLVGNKINVGHSQKIYKHFKDLEGKIEERLQNRQAE
                     YQNEINQPSAPGVNF"
BASE COUNT        18828 a        20379 c        18808 g        17620 t
ORIGIN      
        1 ataacgaccc ggcctgactg aatatctcac gttttcctgc ttaacattca tgttatgatg
       61 ctcttgaaac taataatacg tatggaattg tcactattta tctgctaatg aattcaaaca
      121 atgtcattcg tttgatgaat atattatttc agggaataat tatgacacct ttagaacgca
      181 ttacccaact tgttaatatc aatggtgatg ttaataatcc tgatactcct cgtccattgt
      241 tatctcttga ggattttttt attgacaata atatccatgg ttcaatatgt tgcaatgtga
      301 ttccagaaca atctccgcaa gctatctatc accattttct gaaaatacgt gagcgtaaca
      361 atgttagtga tgttttggta gaaattacga tgttcgatga cccagactgg ccctttagtg
      421 aaagtatttt agttatcacc actgcttcac cagaagaagt tcagagctgg tttgtagaag
      481 aaattgcccc tgatgagtgc tgggaaggat ggtcagaaga tacggagcat ggatgggttg
      541 aagtccctgt gggtatgcat ccggttactt gttggtggga ttaaaacatt tatataaaca
      601 gataagcgga tgtttacata aatttttatg tgaaggaaca tgatgaaact tattcctcgc
      661 agtagtgata tttcacctgg cattgatgga atttgcccgg ggccttttcc tccgaacgga
      721 tttaccgtac ttacagatgc agcatatggt aacggagact gtttcggttt gtactggcct
      781 attggtcagg agcataaact acctatagtg tgcgaaacgt accatgacga atggcgaatc
      841 gtacctgcct tttccagcat taagaagttc gaagaatggc tggaagtgaa tgatgatgat
      901 ccccatgaaa atggcatcag catcgaagat caagactttg cagcaaatct cttccgtgtt
      961 gctcggaaat gtctttcaac gggaaggctt gatgacgcac tgccactgtt acagcgagca
     1021 acagaacaat tgcctgaagt cagcgaatat tggttagcac tggcaatcca gtatcgccga
     1081 tgtaaaaaaa cagaagctgc agcacaggct gcgcttaatg cttatcttgg caattgggca
     1141 tttggtgttc ctgacaataa agtcattcat ttgctgtcac aggccgcaga tgtcccgaat
     1201 ttccaggatg atcctgtgat tcagtgcatt aaagaacaag gattagatct tagctttgga
     1261 ggaacaaagg aaaataataa ttaccccttg atgcagatgt gtgttgatac ttattttgcg
     1321 cagagaaaac cgcttcaggc attaaccctg ttacataatt atgcctggat aatgtccagt
     1381 gaaacgactg catttcagga acgctatgat tttaatatcg atgaatggcg ggcaaaattc
     1441 aggcagttat gtttagagta ttttggcgat agtcgtacac agttcactta acgctctgcg
     1501 tctatctact atcccttctt tttcagaaaa accgccgagc atgtcggcgg ctttctgact
     1561 tacaaagtaa cagattacat cagcggcatt gccaaacgca caatgctaat cagcggttgt
     1621 ggccatacac ccagcaccag taccaacagt gcagagatca gcacaacgat accgcccgcg
     1681 ctgtactgcc agtttgatgg tgcatcgcga cccggttgtt ccggggcgtg aagatacagg
     1741 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1801 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     1861 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     1921 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     1981 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2041 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgct
     2101 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatattggtc
     2161 tggctcagcg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagcacc
     2221 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2281 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2341 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2401 atcatcaggc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccaaggttt
     2461 ttacccaacg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccaaac
     2521 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagtgaa
     2581 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcgata
     2641 ccgaggaaca gagacgccag atggttggca ttcgccagca ggatcccgcc cagcgcggca
     2701 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2761 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     2821 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     2881 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     2941 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3001 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3061 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3121 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3181 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3241 gcgcgatgta acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3301 acggtaatca ccggtacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3361 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3421 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3481 taaagctgac cacaaagaat aaacagaccc gccgccgaca aaccgtgcgc aatcatctgg
     3541 attaccgcgc cctggtaggc caactggctg ccggtgtaga tagcaatcag cacgaagccc
     3601 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgggc gaaggccatc
     3661 cacgcaccgt agaagatgcc gataacaccc agccacatag caattggcgc gaactctgcc
     3721 gacgcgttcg ggaacagcgg cagggagaaa cgcagcaaac cgtaagcggc agttttcagc
     3781 aagatccccg cgaggtcaac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     3841 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatt
     3901 aacaggtatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     3961 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcaat caacatcacc
     4021 aggccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4081 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4141 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4201 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4261 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagcccgtca
     4321 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4381 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4441 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4501 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4561 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4621 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcaccg cgaaggcggc
     4681 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4741 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4801 cagatagccg ttctcactta acagcagacc tttacctgca aagcgggaaa ggacagccgg
     4861 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     4921 cttgacgaac actttgtcat acagccagtc aaatccccag gcgttgtacc accaggtgcc
     4981 cagcagacgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt tacccagcca
     5041 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5101 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5161 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5221 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5281 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5341 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5401 cagcggtagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5461 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5521 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgggtcatca agtggaaaat
     5581 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5641 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5701 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5761 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atggttgcgg cgtggatcag
     5821 cgcggagaca ggcgtcgggc ccgccatcgc gtcggcaagc catgtctgca acggcaactg
     5881 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     5941 gccgtcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca gggtgcccag
     6001 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaacacg tcacccacac gggtcacgac
     6061 gaacgctttc attgccgctg cgccattctt cggatcggtg taatagaacc cgatcagcag
     6121 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tgtcggcaag
     6181 caccagaacc accatgctgg cgatgaacag gttggtgtaa gcgaagaagc gagagtagcc
     6241 ctcttcaccg cgcatatacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6301 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6361 gcctaccgac atccacgtcc acagcggctg gctgtatgtc tgctcgccgt tagcgaagaa
     6421 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccta cgccgacgat
     6481 cgccgagacg ttttcagacc agcgcccacg ggagaatgcc agcaggacga agccaatcaa
     6541 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6601 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6661 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg gccccagtag
     6721 cttccggcga ccacgaaggc cagcgcggag gcgttaatca tgatttccag accaatcaac
     6781 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     6841 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     6901 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     6961 ccacaaccag acctgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7021 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7081 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccggac
     7141 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7201 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7261 ccagcgcacc ggcgaagtaa gcgcccagtg agaagaacac cccggaaatc gccagcagcg
     7321 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7381 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7441 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7501 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7561 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7621 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7681 gcagcgtgag aagttgatgc ggaaaaattc cgggtaccag cgaccgtctt tggtttctgc
     7741 tttttgcagc gagatacagc cgaccgggca ggctaccgcg cagaggttac aggctacgca
     7801 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     7861 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     7921 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     7981 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8041 agcgtcagcg gcaggcagat tttccagccg aaggacatta cctggtcata acgcggacgc
     8101 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8161 cagatgaatg gcggtaacaa cgggccttgc caaccaccga agaacagcgt caccatcaat
     8221 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8281 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8341 tgacggtgac ataccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8401 ataacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8461 tgcgccacca cgcccatcaa ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8521 gaagcacgca tcgcacccag caacgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8581 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8641 gcaaccaccc aacccggact gactggcaca atcgcaaagg ccagcagcag cgaggtaaag
     8701 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8761 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     8821 ttaggtccgt aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     8881 gccccgcagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     8941 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9001 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9061 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9121 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9181 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9241 ggtggtaata cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9301 tggtgaagta atccagacca ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9361 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9421 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcagt cggctggttg ttaccttcca
     9481 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9541 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9601 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc tgccagttcc gggattttcg
     9661 ccacaacagc gtcaatcaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaggg
     9721 tgctgtgcag cgagtgtaac cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9781 aagcaggatc gtaaacctgg aagaaacgtt gggcgcggcc ttcgttgttg atcaccgtac
     9841 cgtcgctttc agcaaagctg gcagcagaaa gtaccagatg ggcgttttcc ataatcgctg
     9901 tgcgttgatg atcaaccacc atcaccagcg gtgctttagc cagcgcagca ttcacgcgga
     9961 tagcagaagc gtgacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10021 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10081 tgttgacgga acgggcaatc atggtgatac cgacgtcagc gccgcgacct ttcagggctt
    10141 tcgcgacgtt agccgccgcc tgaatcacct ctaagctacc ggcgttcgtc ccggagataa
    10201 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ctttgcagct
    10261 caggttcgat accgtcaacc gctggtgcag agttatccag cgcatgggcg atggcaaaac
    10321 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10381 gggtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10441 ttgccgcaat ctgccagtca gccactttct gtgctgccgc catttcgcgc gctttacctt
    10501 tcacagcctg acgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10561 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10621 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10681 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10741 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10801 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    10861 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    10921 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    10981 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11041 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11101 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11161 tgtcgtgcgc accgtaaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11221 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11281 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacag
    11341 tcatatcctg aagatggcag ttaccgccct cttcacatac cggacagtcg tgcgggtggt
    11401 tggtcatcaa ccactcgacc acgctttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11461 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11521 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgaca cttcccagcg
    11581 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11641 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11701 ttccagtttt ctcagtcaga gactgagcgt taatcgaaat tcggttacca gcgctctttc
    11761 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    11821 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    11881 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    11941 atatcgcccg gctgaccttc accacgctcc agcgcacgca gaattttcac gctccacggc
    12001 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12061 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12121 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12181 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12241 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12301 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12361 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12421 gtttcgacgt tgttgacaca ggtcggttta ccccatgcgc cggaggttgc cgggaagggt
    12481 ggcttcgagc gtgggttagc acgacgtcct tccagggagt tgattaacgc tgtttcttcc
    12541 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaaatcgaa acctgttccc
    12601 ataatgtttt tgccaagcag acccgcttcg gtggcttcgg caatggcacg gcgcagatta
    12661 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12721 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12781 tctttatagg tgcccggctc catttcatcg gcattacaca gcaggtaacg gatgttcatg
    12841 gattcgtctt tcggcatcag gctccatttc aggccagtcg agaagcccgc gccgccgcgc
    12901 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    12961 gccttacgcg cgccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13021 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13081 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13141 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13201 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13261 cctgaatacc ctgataaccg ttgatatgac agaccacgct gtcacaataa cggatcacat
    13321 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13381 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13441 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13501 gcatctcgtg ctcgatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13561 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13621 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13681 atgcgcaaag ctcggggtac gaacacgggt gcggtaactc atggtgctgc cgtcgctggt
    13741 caggtagtaa ctgttgatcc ctttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13801 catcaccgga ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    13861 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    13921 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttaag
    13981 cattacgcgg gtgtagcagt cagaaacgcc accacccacc gggatttcaa agtcgaagtt
    14041 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14101 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14161 ggaacgacct ttcagaatgg tgttttgcag cgccgctttc tcgtaagacg ccagacgttt
    14221 cggcatccag tcgaggaact cacgcagcag gcgatcccag ccgcgcggca ggtcgtgcgc
    14281 tacgccgcca atacggaacc acgccgggtg catacggaaa ccagtgattg cttccaccag
    14341 atcgtaaatt ttctgacgat cggtaaaggc gaagaacact ggcgtcattg cgccgacgtc
    14401 ctgaataaag gtcgagatat acagcaggtg actgttgatg cggaacagtt cggagagcat
    14461 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14521 cacgtaaggc atttcgttaa cgcagccgcc gaggtattcg atacggtcag tatacggaat
    14581 gtagctgtgc caggactggc gttcgcccat tttctccgca ccacggtggt ggtaaccgat
    14641 gtctggtacg cagtcgacaa tctcttcgcc atcgagttgc aaaacgatac ggaaagcccc
    14701 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt tttcggtgcc
    14761 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    14821 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccggataat ctttacgcag
    14881 cgggtgacct ttccaggttt gcggcatcat gatgcgtcgc aggttcgggt gaccgtcgaa
    14941 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15001 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15061 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15121 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15181 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15241 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15301 ttcgccaatc accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15361 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15421 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15481 aatctcgtca ggtgtacgca ggttagttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15541 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15601 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15661 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15721 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15781 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    15841 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    15901 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    15961 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16021 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16081 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16141 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16201 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16261 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16321 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16381 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16441 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16501 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16561 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16621 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16681 aaaaacctta caatcactgt agaaattctt ttatacagct aattgatgtg gttttttact
    16741 cctttctata accttttgtc aactttaaca aaagtttctt cacattagtt tacataatat
    16801 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    16861 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    16921 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    16981 gtcattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17041 gtgatttttt tcaggcgatt atttgtgcgt tcgggacgtg aatctctggt ggttgaaaaa
    17101 tgaacagttt tgtacgttct gcactatgcg atgaaggatt tttactaaaa aaaagccgct
    17161 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17221 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17281 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17341 cagctcatta ttactggacg gatcatagca cagcaaatat tcggtatcag gtagcggcgg
    17401 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17461 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17521 agccagtcgc catggaatat ctgctttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17581 gctaggatca tccagcaata caagagggat cggctcgcct ttttgcaata tgtattccgc
    17641 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17701 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17761 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    17821 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaata cgccctgaag
    17881 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    17941 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18001 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18061 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18121 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18181 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18241 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18301 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18361 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18421 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18481 gcgaacacgt accaacagca atggtgaggc ggcatcaaga gcagggatcc gtcatttatc
    18541 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18601 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18661 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18721 ttcacacctt gcccccattg cgacacctcg gaaatcatca cgcagttaaa ctctccgtaa
    18781 tgaggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    18841 ttatcttaca gataaaaaaa ccagacttac aattaagaat cagaacaatt cactatataa
    18901 cattgcatgt aaagcatata cacctcatta ttttgtcatt attaagttat taacagcaca
    18961 atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg
    19021 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
    19081 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg
    19141 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
    19201 gaacatcggc aacccagccc cgttcggttt tgacgcgcca gatgaaatcc tcgttgacgt
    19261 gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaaggtcttt actccgcgcg
    19321 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
    19381 ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
    19441 cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg cggtttcgct
    19501 ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
    19561 cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg atcgttatta tcaacccaaa
    19621 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg
    19681 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgacgc
    19741 tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct ttaacggact
    19801 gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
    19861 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
    19921 taacgttcct gcgcaacacg ccattcagac cgcgctaggt ggttatcaga gcatcagtga
    19981 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa
    20041 cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat
    20101 cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg gtgctggatt tcctgttgca
    20161 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
    20221 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
    20281 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagc
    20341 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
    20401 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct catgcgcaac
    20461 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
    20521 gcagctatca aaaatcgaaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
    20581 gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac
    20641 ttcaattcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcaaaaactg
    20701 gtcgatatgg ttccggaaga gctgcgggat atctttgcgc cgttaattga cgagcatgca
    20761 tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa
    20821 tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac gcgactggaa
    20881 gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaaat atttgttccc
    20941 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
    21001 tgcgtcgcat caggcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
    21061 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
    21121 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagtgtgtta acaggtgaag
    21181 aaaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgcg aatggataag
    21241 gcgacacccc catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
    21301 cggtattaga gataaatagc ccaataaccg ccgacaagac aaacaaacac cccagcatca
    21361 tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt caccgccagc gcgacacctc
    21421 ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata atgctcggcc
    21481 agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc
    21541 aggcgatgat agcggcaaca ggattaggaa tttcatctgt cagcattaac gccaccatta
    21601 gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggtgatgct tcactcacct
    21661 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
    21721 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
    21781 cttccagcgc cacgccattg cgctttagcc ccaccacatt cagtccgtag cgggtacgaa
    21841 aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg
    21901 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
    21961 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
    22021 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
    22081 gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat
    22141 ccagccgttg accaatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
    22201 tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg
    22261 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
    22321 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
    22381 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
    22441 taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgacgtt tgcatacgca
    22501 tggcaacgct taacaccacg ggaataaaga tagcgacaac gccggttgaa ctcataaacg
    22561 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctattgcccg
    22621 caactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc
    22681 caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg gaaaatactt
    22741 ctgggactgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
    22801 ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc
    22861 aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact tgattctgcc
    22921 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
    22981 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
    23041 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatgac aacccgccgc
    23101 caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct gcggcgcaag
    23161 ccccagcagc tgcgcgccta acagatacgc atcaggttct ggttttccgc gcttcactcg
    23221 ctcagcggtt acaaacacct ctggtgcggg aagcccagct attttatggc gcgctcgcgc
    23281 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgact
    23341 gagtaaggcg attgcccccg gaagcgcggt aataccttcg gtttccgtgg cctcgatgtg
    23401 ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg
    23461 cagagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg gcgctaaccc
    23521 atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt
    23581 tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcattgataa
    23641 tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa
    23701 gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccgtga gttccggcgt
    23761 caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg aggtaacgtg
    23821 cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt
    23881 cctgcaaatt agaccaggaa acatgcaacg catgatacat ctccataatg tcgatgatag
    23941 tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc atctgtaatc
    24001 cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga
    24061 gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg gtcatttcca
    24121 ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt atgccttctt
    24181 ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc cagttgtttg
    24241 aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc aacgggtgcc
    24301 aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt aaggattgca
    24361 gggggtaatg gcgtgacaga acgcttgccc agccaccaca atccctgcat gggtaaactt
    24421 aaggcgaaca gggcagtggc aactgccggc ccaagctgac cgcccagggc aatctgccag
    24481 cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt catcttgata
    24541 acgcgatttt cgacaaagac cggggcaagg cgtttttcca gcggccacgt ctttgagtaa
    24601 tgctgtcccc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg cgttgacatg
    24661 cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca actcagcggg
    24721 acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta gcagcctgaa
    24781 ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc cattggctga
    24841 aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa aaaatttgca
    24901 gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat ggctccctga
    24961 cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt agtactggtt
    25021 ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa tggtgaagag
    25081 tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa atggaaaatg
    25141 gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga agcgctcaac
    25201 tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc
    25261 ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat cgatgagtct
    25321 gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg
    25381 atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa cgttgctgta
    25441 tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc cctgccttac
    25501 aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag ccacttctat
    25561 gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa catcatcacc
    25621 tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg cgttgacacc
    25681 tctatgggcc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg tgatatcgat
    25741 ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa
    25801 ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga ctgccgctat
    25861 gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt ttactgccac
    25921 cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct ggacgctgtt
    25981 gtattcactg gtggtatcgg tgaaaatgcc gcaatggttc gtgaactgtc tctgggcaaa
    26041 ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg tttcggcaaa
    26101 tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac caacgaagaa
    26161 ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc cagctcagct
    26221 ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa accgtgtccc
    26281 gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc agccttggcg
    26341 tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc
    26401 cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg aactcttcca
    26461 ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt tccagcaatc
    26521 agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc aaagacgctg
    26581 aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc cagtctctga
    26641 actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg
    26701 acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc ggcggtgcca
    26761 aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt gatgaacagg
    26821 gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct aaagtaaaca
    26881 atgttgatcc ggcgaagctg caagaatcca gcccgctgcc ggttctcggc gctgtgccgt
    26941 ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg aatgcgacca
    27001 tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca
    27061 gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact tccgcagacc
    27121 gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc
    27181 tgctgctgac tggcggttac gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt
    27241 tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc tctctgagcc
    27301 tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag aaagttcagg
    27361 aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc acttctgagc
    27421 gcagccgtcg tctgtctccg cctgcgttcc gttatcagct gactgaactt gcgcgcaaag
    27481 cgggcaaacg tatcgtactg ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta
    27541 tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca gagatcaacc
    27601 gtgttgcagc gtctcagggt gtagaactgg gtgcagggat tgaaatcgtt gatccagaag
    27661 tggttcgcga aagctatgtt ggtcgtctgg tcgaactgcg taagaacaaa ggcatgaccg
    27721 aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg atgctggaac
    27781 aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac accatccgtc
    27841 cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc gtgttcttca
    27901 tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg gatccgaccg
    27961 ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg
    28021 aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt agcgacgtag
    28081 aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg
    28141 gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact
    28201 ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc ggtaacacca
    28261 cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg ctgcagggta
    28321 tgcgcaagcc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc gtctacacca
    28381 tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat ccgcagcttt
    28441 gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt ctcttaattt
    28501 gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt gcgccatccc
    28561 actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg tccgcaatca
    28621 ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt atcttttttg
    28681 ttgctatttt aaccagcctt gccacctggg tagttccggt ggggatgttt gacagtcagg
    28741 aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat
    28801 ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga
    28861 cgggcgatga acgcccgggc ctgatgaact tcccgtttga aggattaacc tcaggatcga
    28921 aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc gcgtttggca
    28981 ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc catacccgcg
    29041 ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat
    29101 ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta atggtccggc
    29161 tgggctatga cagtattacc accgtcctgg tgacctatat tgccacgcaa atcggttttg
    29221 ccagttcgtg gatgaacccg ttttgtgtgg tcgttgctca ggggattgcc ggcgttccgg
    29281 tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg attggcctga
    29341 tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg
    29401 agtccgaccg cttctttcgt gaaaagcagg ctgatgttga acaacgtccg tttacctttg
    29461 gtgactggct ggtattgatt gtcctgaccg ccgtaatggt ctgggtgatt tggggcgtga
    29521 tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg ggtctggtga
    29581 ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc atggcttcat
    29641 cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt ttcgccaaag
    29701 ggattttgct gctggtcggt aatggtgaag cgggtgatgc cagcgtgtta aataccatcc
    29761 tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc tggtttatgt
    29821 tgctcttcca ggcggtattt aatttcttcg tgacgtccgg ttctggtcag gcggcgttaa
    29881 ccatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc
    29941 tggcgttcca gtttggtgat ggcttcagtc acatcattta cccaacctca gcgtcgttaa
    30001 tggcaacgct cggtgtgtgc agggtggact tccgtaactg gctgaaggtg ggcgcgaccc
    30061 tacttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag ttgatgggct
    30121 accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc gtttcagtct
    30181 ctacggcttc atttttggca ttgcgcttca tccacaacgc tagcgctttc agcgagtctg
    30241 gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa acttcactga
    30301 cttcatcttc ctgtagtgcg aagggaccgt gagagacgca gctgaacaat gcgccccaga
    30361 cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg acaccggcaa
    30421 tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca tcggcctgga
    30481 ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac
    30541 gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca cgatgacgca
    30601 gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc tcttcattga
    30661 caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg aaaccttctt
    30721 tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta atcgcgacct
    30781 gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta tccagcatgc
    30841 cataactcgc cggattaccg cctttcggaa tactcaccga gccggggttg aagtggaaaa
    30901 tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc acatcgttct
    30961 ggtttaaagc aggcagattt tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt
    31021 gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc agcatttgat
    31081 ccacttcgct gtcgcagttg ccgcgcacag cgataacctt atgtgccact tcattaagcc
    31141 gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg ccgtgattca
    31201 acacgtcgcc aaggatcacc agccactggg caccgctttg ggcaaacaac tccagaacac
    31261 gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact
    31321 cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc
    31381 ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa tgttgcataa
    31441 tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc
    31501 agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag tactaacaaa
    31561 tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct
    31621 tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg
    31681 ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa
    31741 atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat
    31801 tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct
    31861 tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac
    31921 aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca
    31981 tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc
    32041 tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc
    32101 gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct
    32161 atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagctgg
    32221 attatcgctt gattaaggta gacctgggga aaggcggtca gtttcgtccg gaatttttgc
    32281 gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg
    32341 aaccgctaag cctctttgag tctggtgcca ttttgttgta tctggctgag aaaacaggac
    32401 tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg
    32461 taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa
    32521 ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactga
    32581 acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg
    32641 cctgctggcc gtgggttaat gcctggactc gccagcgaat tgacctagca atgtatccgg
    32701 cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa
    32761 aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt
    32821 tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat
    32881 aaagaacctt cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca
    32941 ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga
    33001 tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa
    33061 taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca
    33121 tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga
    33181 ttcggtatcc atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac
    33241 atagtgatca ccggagggac aggattaatt ggtcgccatt tgattccgcg tttgctggag
    33301 ctgggccatc aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc
    33361 cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg
    33421 gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacga gcaaaaagag
    33481 cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgccagc
    33541 gacacgccac cgtcggtact catttccggc tcggcaacgg gctattatgg tgacttaggt
    33601 gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttacccataa actctgcgct
    33661 cgctgggaag aaattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcgt
    33721 accggtgtag tgctggcacc ggatggcggt attctcggta aaatgctgcc gccgtttcgt
    33781 cttggcctgg gcgggccgat tggctctggt cggcagtatc tggcctggat tcatattgat
    33841 gatatggtca acggtattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg
    33901 gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat
    33961 cgcccggcca ttttgcgcgt ccccgcgacc gccattcgac tgttaatggg cgaatcttca
    34021 gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg
    34081 tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag
    34141 caaacatccg ccagttaact cccggtgtta caggattagt ggctttgcgc gataagatcg
    34201 tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc
    34261 tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat
    34321 ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttccgc tatctcacca
    34381 ataaatgcac gaaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt
    34441 agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc
    34501 aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt
    34561 tgcataatct cgtcatccgg caccacggta atatccacca acggaaaagc cgatgaatat
    34621 attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa
    34681 ggacttctgc aaccatgata aaacagcatc gggagcacca atggaagctc tttatagccc
    34741 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
    34801 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
    34861 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
    34921 ggttccagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
    34981 tcaataaaat cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
    35041 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
    35101 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
    35161 cagaagtatt tttagcggac tgtaactttt acatttcagc ggaagctgac tcgcaaattc
    35221 cccatgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
    35281 aaggaaccgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
    35341 ttcttctatt ttcccctgat ggaggaaaat gacatgagta gaaacatggc gagcaaagcc
    35401 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
    35461 caacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
    35521 ttccggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
    35581 atgcaccgga tatttcccct gcgcacgttc gtctatcccg acttttgcca gatacttcac
    35641 cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
    35701 gttttccagc accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
    35761 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
    35821 caccagattg atcgtctggc cattgaccac gatcgaccct tcactcggtt tttcgaggaa
    35881 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
    35941 atctccggca ttcgcttgca gtgatacccc tttcagcact tcatgttcgc cgtagcgttt
    36001 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
    36061 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
    36121 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
    36181 cgttaatatc gcgggctatt ttcagcagat ccggcaccgt ggcagtaaat gccaacgcag
    36241 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
    36301 aaggcaaaat aatgcagcga tacattttaa aagtcgagaa gccataggct ctggcggctt
    36361 caatttcccc atgcgaaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
    36421 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
    36481 ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact
    36541 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
    36601 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
    36661 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
    36721 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
    36781 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacagtgg tgaaaaccag
    36841 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
    36901 ttgggtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
    36961 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg acggaaacat
    37021 gatccgccga aacacttgcc cacgagtaaa accgaacgcc gtcgccgcct ctatatgtcc
    37081 tttcggcact gccataaaag caccacggaa cgtttcggta aaataagcac cgtagataaa
    37141 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
    37201 ctccgtcacc gtgtttagcg caatctgcaa accgtagaaa atcagcagca tcaacactaa
    37261 gtccggcacg ccacgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
    37321 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
    37381 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa
    37441 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
    37501 gaagggagcg ggctttcttt caccgatggc gattagccac cataaacatc aaaatcgaag
    37561 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
    37621 agtgcttcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
    37681 agtttttcat ctttaacaga cgggccaccg aatttgtaat ctttaccgac aggttgtttg
    37741 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
    37801 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
    37861 caatgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
    37921 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccaa acgagaatcg
    37981 gcagcgtaca gtttgtcggt gaaggctatt tcttgctgac gtttttccgt aatggaaagc
    38041 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcgga
    38101 ttttcgacaa acgtacattg cgtattgatg cgtttgcata attcctttgc cagatcgata
    38161 tcgaagccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
    38221 gtaccgatgc ggatgttttg cggaatcgca gcaaacgccg cagttgcgct ggagaaggcc
    38281 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
    38341 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
    38401 aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta tgcatttcaa
    38461 tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag
    38521 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
    38581 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
    38641 agcatcatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
    38701 tgatgagcca gcaaaggcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
    38761 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
    38821 atcctggttg gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta
    38881 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
    38941 tggctgaatc ggtgaacctt tggccgcaat caaacgagaa tctgcggcgt acagcttgtc
    39001 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc
    39061 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca
    39121 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
    39181 acctttagca tctttcgatg agaacggtgc gtaggtggta tcggttccga tacgtaccgt
    39241 ctccggtagc gccgcatagc tggaagccgc tgtggagaga ccgactaaca aagacagagc
    39301 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
    39361 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
    39421 cattcaacgt taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca
    39481 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
    39541 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
    39601 gggcaaagag atcttcagga agggttatcg caaactggtc aagaacacga ttaaccgtct
    39661 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa
    39721 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
    39781 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
    39841 tcagtaagcc atcagtatag ctatggacaa tgccggaaag ggttttgatt gaacagggta
    39901 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggca atatcgcgcg
    39961 catcgtgcgt gacatcggct aatgcctgca cttcgcgcag agaaaaatcc gtttcgaggg
    40021 ataaggtctg gcgcgctgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
    40081 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
    40141 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc aggatttcgg
    40201 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
    40261 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
    40321 aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg
    40381 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
    40441 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
    40501 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
    40561 aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
    40621 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
    40681 tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc
    40741 agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
    40801 cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttctggg
    40861 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt
    40921 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
    40981 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
    41041 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
    41101 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
    41161 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
    41221 aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag
    41281 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
    41341 ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
    41401 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
    41461 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
    41521 ctggagctgc cagccgtggg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
    41581 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
    41641 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
    41701 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
    41761 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa
    41821 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc
    41881 agctgtgatt tgctccagtc ttcgcttttc gacaccccgg taaaggagtc gagaaagaag
    41941 agaatggcag caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
    42001 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
    42061 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg
    42121 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
    42181 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc
    42241 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
    42301 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
    42361 gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc
    42421 gactttttat gcctttgcgg catcgggcaa tgcgtgtcgg atgcggcgta aacgccttat
    42481 ccgacctacg gttctacccc tgcgtaggcc tgataagacg cgccagcgtc gcatcaggca
    42541 agaccgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
    42601 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
    42661 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    42721 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
    42781 taggctttac ccgtcggtgc agctttttct tccacgaccg gcttcggttc tggcttcggc
    42841 gcaggtggtg cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg
    42901 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    42961 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
    43021 ggtaacgcct gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc
    43081 accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    43141 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    43201 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    43261 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
    43321 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    43381 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    43441 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    43501 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    43561 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    43621 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
    43681 cgccagcgct gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
    43741 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
    43801 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
    43861 cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct cgcgaccaat
    43921 actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
    43981 gacatcggcg tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
    44041 caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    44101 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    44161 cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg
    44221 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    44281 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac
    44341 gccaagacgc gccgcgacca ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    44401 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    44461 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    44521 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
    44581 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
    44641 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
    44701 caggaattca ctgcgctgga atccaggcgg cagtttttcg cgaacggtct gttcgataac
    44761 acgcggaccg gcaaagccga ttaacgcttt cggttcagcg atgttgagat cgcccagcat
    44821 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    44881 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    44941 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    45001 ttccagcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    45061 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    45121 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    45181 atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc tacccagctc
    45241 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    45301 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    45361 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    45421 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    45481 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    45541 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    45601 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg
    45661 cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc
    45721 atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg
    45781 ccgatcacgt tataagcggc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    45841 ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    45901 ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga
    45961 ttactgaaca acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca
    46021 atcgcggcga tcagcatcag caccaccatc atatggacgt tgagatcgtt ggtttccagt
    46081 gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaataacgg cgttaccacc
    46141 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc
    46201 gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    46261 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    46321 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    46381 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    46441 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    46501 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    46561 atgtacaaaa gcattcgctt taatatctac caccacataa ggaccgtgac gcgtgacatt
    46621 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    46681 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    46741 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    46801 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    46861 tttaacccaa cgcacagcga tgtcaccagg taaattcgca tttacgccca aggtccacgc
    46921 cgcgtctttg cgcagcgcgg ttgtttcgaa atgcacaacc tgcccggtac cgtgtacccc
    46981 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    47041 cttttccagc ttctcctgca cactgcggac ttcgttctgc cgttgccagc cgtaatactt
    47101 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcggttgtt gctggtcgga
    47161 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    47221 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    47281 ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcacctac ctgagttggg
    47341 aattcgttct cttcagagag cacaatatct tcgccttgaa caaacgcatc acgcgcttct
    47401 tctgctgcca gtggacgcag agcttcaaag ttgaccatct gggcatgacc gtagaatacc
    47461 ggtgcctgga cgacgctagc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    47521 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcag taacggcagc
    47581 atgttgaacg ccagctgacg cccgaagaaa tcttcttcgt caatcggaat gccgttgagc
    47641 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    47701 atcaggctgg taacgctgat acgtgataaa ccgccctgat cgattaacgg tttcagtgcc
    47761 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt ccggtaatct
    47821 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    47881 aatccactgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    47941 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    48001 gcatcctgca cggtgattgt cttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    48061 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    48121 gcttcgccca cagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    48181 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    48241 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    48301 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    48361 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    48421 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc gggccatgca
    48481 gcgtaatgcg accaaactct ggcgcaggca gtaatgtatc cagcgcaacg tgctgttcat
    48541 gcccaataaa cttgctataa gcttcaaaca cttgcgtagt accgcgtgct ttaccttcca
    48601 gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tttcagcagc tcgacgttga
    48661 gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct tcattcaggc
    48721 aggtcagcaa cgcagtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    48781 cgggcttcag gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat
    48841 ctttaaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg
    48901 agcggaaatc accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa
    48961 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    49021 ccaccgtacg gtcgtacagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga
    49081 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    49141 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtg ccaacaaatt
    49201 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    49261 catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg gtcacctcac
    49321 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    49381 ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag
    49441 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    49501 cctcctgggc aaccaccagg tcagggagat aatctgccgt ctggcacggg caatcagcct
    49561 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    49621 caacagcaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    49681 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    49741 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    49801 ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    49861 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    49921 atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    49981 cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa
    50041 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    50101 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    50161 acgccgacgc tggaatcact acagatgacg ctaaaacaac ctttagatgc cagtgaactg
    50221 gcggcgttat cggcatatat ccagcaaaaa tatggtcttt ctgcgcaatc atcgctttct
    50281 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    50341 ccgcgtgtta tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    50401 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    50461 ctggttcgtt aaccccgacg aaatgacagt accgtgacaa taatacccag caccgcagag
    50521 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    50581 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    50641 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    50701 catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    50761 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    50821 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    50881 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    50941 ccgataacag caaaaccaac gccttgtagt gccagaccta accctggttt ccagataagc
    51001 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    51061 ccgttacagg cccacgccag tacgggtaat accattgtgg tgatcgccag tgcggcaaaa
    51121 ccgtaatggc tatgaatcaa caggccaagc ggagcaccaa cagcgagggc accgtaaatc
    51181 gccattccgt tccatgacat cactttgcca gagtgttttg gccctacgat gcctagtccc
    51241 caggtcagag cgcctgtcag taactggctt tcaccaaagc caagaatcaa acgcccgacg
    51301 accaacagag caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    51361 ccgccagcca gaccacaagc taacattccc tgaagcgccg aacgttttgc accatattga
    51421 tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg
    51481 gcaatgccga ccatggtatt gccatagccc agttcatgat gaacaaacag cgggataacc
    51541 ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc
    51601 gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    51661 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg
    51721 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    51781 tggaactggc ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    51841 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tctactccga
    51901 cctactgcga attaatcttt cagcttgcgc attaccagcg tggcgttggt gccgccgaag
    51961 ccgaagctgt tagacataac ggtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc
    52021 agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat aaagccgtgt
    52081 tccagcatca gcagagagta gatagcttcc tgtacgccag cagcgcccag agagtgaccg
    52141 gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc acggatagct
    52201 gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gggagttcag gtaatcgatt
    52261 ggggtatcaa cgccatgcat cgccatcttc atgcagcgta ctgcgccttc gccagacgga
    52321 gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc atagatgtga
    52381 gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc gccgccagcg
    52441 ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg ggtgtcgttg
    52501 tatttagtag acagcgcacc cattgcgtcg aattcgcaag ccatttccca gcacagctct
    52561 tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta
    52621 ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac gccatgaatt
    52681 ttaaacgggg tggcgaggca ggcagaaacg ccggatgcca tcgctttggt gaccacatac
    52741 gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa
    52801 cgcggggagc cgccgccgga acctgcaatc aggccaacgc gcgggttatt ctggtaagct
    52861 tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc ataaatggat
    52921 gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc cagttttacg
    52981 ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa agtgatccct
    53041 gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat gctggaaaca
    53101 atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc gcacatagag
    53161 taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat cagccattta
    53221 atagagaaat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc ctgtaagacg
    53281 agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta atgctgaggg
    53341 tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg ggctggaaga
    53401 gacgcgttat gtttttctgg gaggcaacca attagaggta cgctttcctg agcatccaca
    53461 tcctctgttt gtggtagcag agagcggctt cggcaccgga ttaaacttcc tgacgctatg
    53521 gcaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac gcttacattt
    53581 cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc atcaacactg
    53641 gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc ccttgcccgg
    53701 ttgccatcgt ttattgctcg atgaaggccg cgtgacgctg gatttatggt ttggcgatat
    53761 taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg cctggtttct
    53821 ggacggcttt gcgccagcga aaaacccgga tatgtggacg caaaatctgt ttaacgccat
    53881 ggcaaggttg gcgcgtccgg gcggcacgct ggcgacattt acgtctgccg gttttgtccg
    53941 ccgcggtttg caggacgccg gattcacgat gcaaaaacgt aagggctttg ggcgcaaacg
    54001 ggaaatgctt tgcggggtga tggaacagac attaccgctc ccctgctccg cgccgtggtt
    54061 taaccgcacg ggcagcagca aacgggaagc ggcgattatc ggcggtggta ttgccagcgc
    54121 gttgttgtcg ctggcgctat tacggcgcgg ctggcaggta acgctttatt gcgcggatga
    54181 ggcccccgca ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt tattaagcaa
    54241 acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc gtcggtttta
    54301 cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt taggctggga
    54361 tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg cagaactggc
    54421 tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa attgcagcgg
    54481 cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta atgtgctgga
    54541 actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga atttatcccg
    54601 taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac acagcgtagt
    54661 ggtactggcg aacgggcatc aaatcagccg attcagccaa acgtcgactc tcccggtgta
    54721 ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaattggcag agctgaagca
    54781 ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac atcattgtat
    54841 tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg atcagcagca
    54901 gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag aggttgatgt
    54961 cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc tgccaatggt
    55021 aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg cggagcagaa
    55081 agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg ctttaggttc
    55141 tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga tgagcgacga
    55201 accgattccg atggatgcca gtacgctggc agcgttaaac ccgaatcggt tatgggtgcg
    55261 gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag tgtttgccgg
    55321 atgcggcgtg aacgccttat ccgacctacg tgtgacgtgt aggcctgata agacgcgata
    55381 agcgtcgcat caggcatggt gctccagacg ccgcaactta ctgctgtgac gcctgctgaa
    55441 acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat tcaccggcac
    55501 caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg gagtggtcat
    55561 caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac ccactggcaa
    55621 acaactcatc atcactggca tggtcaacca taccgtcgat taatgccaga atgcgtgatt
    55681 caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcaccggctt cagttgagcg
    55741 cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa ttttgtagtg
    55801 ctttaatgaa gcgtgccgga cgctcgggaa tgccatttgt cagataatcc atcacctgcg
    55861 catgtacccg ccgttgaaac accacacgat ccggttcgaa gtcgccttcc agattgtcgc
    55921 aactgacgtt aaacggatat cccgccgcca cacagaacaa ccagtcgagt gcctgcggct
    55981 tcacttcaac atcttcaaac tggctttgcg tttgggcatc acgcccatcc gggcaatacc
    56041 agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag tgcgaaatct
    56101 catgaattgc gctggcataa aagccgtggg caaagacaat tcggttatac ggtacttccg
    56161 catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta ttaaattcat
    56221 cggcaaagca gctattaaaa atttcaatca actgctcata gtggtgtgta ctgttcatta
    56281 gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta atttggcact
    56341 catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt tgctcaacac
    56401 cagccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa agcccgtcgc
    56461 ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg acgttgcgtt
    56521 gagtaatttg gcgtgagccg tggctttggc gaggttaaat ccgcacagcg taacgaaggc
    56581 cagcgcgtaa aacgacccgg cagccgggcc aaagaatcca tcataaaaac cgacgcagcc
    56641 accggctatt aacgcgaagg gtaaaccgta catccggcgc tggcggtctt cttcacctag
    56701 ctttggcatc agcaagaaat agaggccgat acaaattacc agaatgggca aaatctgccg
    56761 taagacatca gcctgaacgt attgcaccag cagtgcgccg ctcattgagc cgacaaaggt
    56821 catggcgata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa taaagtagat
    56881 agtagcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg cattagcggg
    56941 agacatcccc gctgccatca atgccggaat ggtgagtaac ccaccgccac cggcaatcga
    57001 gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca gcggggaaac
    57061 cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag cgcctggcag
    57121 gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg ttttggaggt
    57181 tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa aggttgatct
    57241 tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc gcgatgctgg
    57301 aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag gcaaagttgt
    57361 tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc ggcgagtttg
    57421 atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc gtcgcgggaa
    57481 actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt tttcggcagt
    57541 tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc gttgaaacgc
    57601 ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag attgctcacc
    57661 tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata gcgacgctga
    57721 tcggtacgca tgacctgata atgttcggac tgtatcggca gcgtgtcagc gccgacaata
    57781 cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg ggttattttt
    57841 tgccacggcg tcgctgccag gctggcgcta ctggcaagca gagccagcag cgcaatcgcg
    57901 gttttattca ttttttacca gcgtggaata tcagtcttca catcggcatt ttgcgcccgt
    57961 tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttctgcgat cggcactgcg
    58021 cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc ttcgccaaag
    58081 cggttaatgg tacgacccgg cacggtaatg ctggaggtcg gtttcagcgc catatgggca
    58141 atgatttgct gcccgctgct gataccgccg agaatgccgc ccgcatggtt gctctggaaa
    58201 ccgtctttgg tgatttcatc gcggttctgg ctgccgcgca gcgccaccac gtcaaagccg
    58261 tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc gatgtcagca
    58321 tccaggcggt caaagaccgg ctcgccaagt ccggcaggaa cgccactggc aacaacggtg
    58381 actttagcgc cgatggagtc gccctctttt ttcagcgcac gcatcaactc gtctaacgcg
    58441 tcgattttgt cggggtccgg gcaaaaaaac ggattttgct cgacctgcga ccagtctttg
    58501 atatccagcg gaatgtcgcc catctgggtc aggcagccac ggatttcaat accaaatttc
    58561 tcggcgagat attttttggc aatagctcct gccgccacgc gcatggcggt ttcgcgggcg
    58621 gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta ggtgtaatcg
    58681 gcatggcctg gacggaaaac gtccttaatc gcactgtaat cctgagagcg ctggtcagtg
    58741 ttttcgatca acaagccaat gctggtgccg gtagtaacgc cttcaaaaac accggagaga
    58801 attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc agggcgacga
    58861 cggtcgaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg aacaccatcg
    58921 acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg aaagagttgt
    58981 ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt ttacttaatc
    59041 tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttggtga gcataaacac
    59101 accatcgccg ccgttatcaa actccagcca ggtgaacgga acatccggat attgttccat
    59161 aagatgtacc atgctgttgc cgacttcaca aatcaacacg ccatcatcag caaggtaatc
    59221 tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcagtgccag atgccaggcc
    59281 cagttccggc tcgtggcggt attcgtttgg caggtcggac atatcttccg catcgacata
    59341 cggcgggtta gtgacaatca ggtcgtactg cactttcggc aagtcgcgga acagatcgga
    59401 acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt cagcaaccgc
    59461 cagcgcgtct ggagagatat ccaccgcgtc gacttctgca tccgggaagg cataagcaca
    59521 ggcaatggcg atgcagccgc taccagtaca catatctaaa atatgctgcg gttgcttgct
    59581 gataagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg gcaccagcac
    59641 gcgttcatcg acgtaaaatt catggccgca gaaccacgct ttgttggtca ggtaagccac
    59701 cggaatgcgt tcattgacgc ggcggatcac gcgttcaaca atacggtgtt tttcgctgga
    59761 ggtcagacgc gcggtgcgca tatcttccgg aatatccagc ggcaggtaga gcgaaggcaa
    59821 caccagctgt acggcttcat cccacgggtt atcggtaccg tgaccgtacc agatatttgc
    59881 cgcgctgaag cggctcaccg accagcgcaa catgtcctga atggtttgca gctcatttac
    59941 tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc aataatttcg
    60001 gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg agtgaggaaa
    60061 aatacgttta aaacgatcga ttgcgctacg agtcgggtaa actgtaggaa aattagaaat
    60121 agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc tctgtttcgc
    60181 cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg accgcagcgt
    60241 aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga tgccagccat
    60301 tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa atatgttcgc
    60361 ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc accggaattg
    60421 tttttggatt tacacggtct gacgcagctg caagctaagc aggaactggg cgcgttgatt
    60481 gccgcctgcc gccgtgaaca tgtgttttgc gcctgcgtga tgcatgggca cgggaagcat
    60541 attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat ggcctttcat
    60601 caggcaccaa aagagtatgg tggtgatgcg gcgttgctgg tattgattga agtggaagag
    60661 tggctgccgc cggagttacc ctgagaagtt tagcgcgttg cgccatgtac ggggaatggc
    60721 agggtcaaat gaccctgcct tgtgaaaaag acgcactagt acatattcag tatgtaagtg
    60781 aaccggcagg tggcatctat tggctgttgc gccgttgtgt ccacgcgtag cttcatatca
    60841 tgcagttgcc tggacgtgat cggttgcggc tctggcgtca ctgtcacttt ggcatcgtca
    60901 cagcccgaag tggagaaggt catctgctgt gcgacaaaac ggcagtaagc accacgctcg
    60961 tcatatttac taccatcaca cagttttcca ccttccatca gataagtggg cagcgtttgt
    61021 tcaatcacag cacctgttga ttgcatcctg actattttgt tatgcacgac cgtattttca
    61081 ttccaggaga catcataagg atctaaaggt attgcgctac agtcagaact gacttttgtc
    61141 gggccaatat agggaacatt attacctgta tatattgcct ccgttgcgtc ctcactcttt
    61201 acatacgagg aaactatcca cggacaacca tgcatatcac cgtcatagaa aactgtgact
    61261 ttcatttcca cgcaggccca ggtcgtttgt ggggtgcgag catcatattc ttcaaggcca
    61321 gggcaacgtg aagttggtaa tatggttctt gcgtcttcta aatgcagttc cggagcagta
    61381 ccttgaccag tccatgatac tagtggcaca ttcaaaatcg caactgacct ggattgatat
    61441 accccatatt gcgcgccttc tggtgtgagg atagaaagat taaactgaaa acctgtgagc
    61501 actcccattg atcccgcagg aatgtataac gtcgcgatct ttttcgcggc ctgcactcct
    61561 gtgctgaggc aaatgagcag caaaaagtaa aagacataac gtagaatttt cattttaaat
    61621 atactcgaag gtagcggtca ggctggcagt gaattcaccg atcgtcacat cgcgaccgct
    61681 tttcgcctgg atccaggccc ggaaattaat atcattgttg ccgttggtaa gcgcaaaagt
    61741 cgccccggta gtgttgttaa tatccacccg cttgccgtcg agcgtttcca tccctatccc
    61801 cacgccctgc gcggtggagg tgtcatcgac tgccagaaaa cccggctgac cgctgtcttc
    61861 ggtgccgctc agcgtgactt tgacgttgta gcccgctggc cctttgcagt ctttcagctt
    61921 aaacacaacc ggcagatgca cagactgtcc ggcgactatc aaatcccggt tgctgacggt
    61981 gggaaaatgg atttctgcca gattggcacc atccaccacc agcgtgcagg atttactcag
    62041 caggttgccg taaaaatgca ggttgttatc gactgcccgt gcccccgtgc tggcggtcag
    62101 cagcaggcct gctaacagcg cataaccggg tgaataaatg tgtgctgttt tcattggtaa
    62161 tccacccgta aggttgcgta agcagaaagg gtcgtgtccg tcagcaccgc atcagccgct
    62221 ttgaccggta ccgcctccag ctttggcttc tggctaaaat cggtggcgtt aatggaaagc
    62281 ggctcgttga gcttaaaacg ctgaccgtca tgctgaagct caattccgaa gcccggtaca
    62341 tccgtctcaa tagcagagtc gttgtaggag gtggcagtcc ccgtccatga gagtgtcata
    62401 ttccactcgg gatcgcggac atcagactca cagctcagtt cgtagtccac ttcctgacga
    62461 ccataattgc cgtcaatgct gtcgataatg agatcgcgaa attgcacttc gatggtttgc
    62521 ccgccgctga tgctacaggc gggcggcgac atcagcgtac cgtggaatgt gatgtcatct
    62581 tccgccgcca gcacagcccc gcaaccagcg cacagtaaaa ggagcaggaa ttttttcatt
    62641 ggtaatccac caccatggtc atcgtggcgt taaactccga cggcgtcagt tgcaccccgc
    62701 tctgttttac cggcaccgct ttcagctgag gctgtgtgtt gacattaaaa ggcgtccagt
    62761 tattttcacc aactgaaaac agggagttgt cggcggcgtt ttctatccgc acccccaggc
    62821 cggcgatatc cgtgctgaga accgtttctc cgttgatcac ggcggtattg cctttgagct
    62881 gcatccgtaa gtcatcgctg actttattac cgcagttgag ggtgtatttc gcatcctgtt
    62941 ggtaattggt accatcgatc ctgttgatca ccacattgcc aaattccacc gcgcttccct
    63001 tcacggagca cggcggcggg ccatcgacca ggattcgcag cgtcagatcc acctgtttga
    63061 tatcgctgcc agcaacggct aaaccgctga caccccatcc ggccagtacc agtagccccg
    63121 cgcccgctaa aaacttgtga ctcataagtc cctcccggca tcagcctttg cggctctgct
    63181 cttcatccac ctggcagttg ttgccggtgc atttgaagta cagcggcatc cgcgcgccgt
    63241 agtcgttgat gtaggtcagc accggcacgc tgtccatctt cgcattcagc ggtgcggagg
    63301 ttttcggctc catcatcagc ggtgtgaatc ccgccggcgg gttgccgcct tttttagcgc
    63361 tggcgttgct gatgatcacg tagtacggcg tcgggttatt gacggtgaag tcctggccgt
    63421 tacggatgag cgtcacctta tgctgccacg gacttttcat gctggtgttc tccagcgcct
    63481 tcggtcgcca gaacagtttg atacgcgtct gcagcgcaat ctgcagtgtg ttcggtttat
    63541 ttgatttcgg cggaatttcg cgcacgttga aatagaacac gctctcgcgg tcggccggca
    63601 gtttgttgat atccggcagc ccctgcacct tcacctggcc gttcatcatc gcatcgatgc
    63661 gctgcactgg cggaagcacc gcaagcggcg agctgatttt gttgcccttt gcatcctcta
    63721 tccagctctg cgccagataa ggcagtttgg gatcgttgtt gcgcagcgtg acgctgatgg
    63781 acttgtcgct ttcgttaaac accagccgtg tcctgtcggg cgtcaccgaa gcgtggctgg
    63841 agaaactgac cgctgtcgcc agcagcgcca gtgccgggac aacgattttt gcagtgttag
    63901 tcagattaaa catggatcat tccttaatca cgttactggt ttactgaaat tgaagtcggg
    63961 ggttccgttg gcgtcacccg ctgtgtctgt tcctgaatcg ccggtttgac cgccggcgaa
    64021 gtgacaggtg ctacattgcc tttatgctgg catggcagta acaagccgtt aaacagatcg
    64081 gcaggcagcg ggtccggcag gttgatatcg caatgcgcaa caccactcca gaacacactc
    64141 atgtgttcgc caggtttcac cccagccaga taaacattgc cgtcatcatc gacaaggccc
    64201 actgtctgct cgttatcatt ttttacttct gcgccaaacg gaggatggct gccgtcttgt
    64261 aaacgcagca ccgccatcgc tttttgacca ctgatgacgg caaatttgcg gtagccaatc
    64321 gccccttcag ttagcgtggc ttgtaccacc gactgggttg cttcggcgtt ttccggcagt
    64381 ttgttgaggt cgatatacgc ctgattgcgg taataattgt taacgtcaga aacgacggct
    64441 ttaccaaaca tattggtata aacagcagcc ccgttacctt ccaccggaac atcggcaacg
    64501 ccatcggcat caatcagcag gcgtgtcccg cccatattct gggtacggtg aagtgcgccg
    64561 ccgtgggtag taagcgttgc gccgccctgt aacgacaagc ccgcagaggt gtattgccct
    64621 tcatggtagt tcgcactgag gtcaacctgc gccagcgaac catcatggct gtagtagcca
    64681 tcaacgctgg tgtgtttgtc actggtgccg atgttcaact gatagtgagt cgcgtcatcg
    64741 acacggctaa aataaccgac ctggctgctg tccgtcccac tgccatagtt gccgttatag
    64801 ctaacggtac tgttgtcgcc ccacggcata ctgagcgaaa tgtacatgcc cttatccgcc
    64861 cggttgtcat actcgtagcg gtagccagtc agggaaacgc tcatattacg aatgctgccc
    64921 atattgaaat agtgggagag catgatgttg tagtttgtct gctcctcacg atcccagtag
    64981 gtatggcggg tatagttgag ataaaccgaa acacccgcat cgcggaagtt ctggttataa
    65041 gtggcggtgt acatctcttt gtcgttgccc gtacggacca tttcgctgtc actggcatcc
    65101 agatactcgc tcatggtcat aaagttctct tccgagaagc gatatccagc gaaagtgacg
    65161 cggctgttga gctggtcaaa gtctttggaa taactcacac ggaaggagtt accgtccagc
    65221 gaacctttgc cataagcggt gtctttatcc agtttggtat gcgaatgagt aacatcaaac
    65281 gcgaccgcgc cgaaggtaga caaatcgcga ccgacgccca gcgccgcaga ctgatagttt
    65341 tcatcaccca gcgcaccgcc atataacgac cagccgttag cgatgcccca ggaggcttca
    65401 gcaccagaga aaaatccccc ctcgacatgg tggccccact cttgcggacg tcccatcatg
    65461 atcttatagc gaacctggcc tgggcgagtg aggtatggca tcgaggcggt gctgatgtca
    65521 tattcctgca cctggccgtt ctgttcttca atgcgaatat gcaacgtacc ggagacggaa
    65581 tcaccaagat cctgaatacg aaacggtcca gccggaacct gcgtttcgta aatcacacgc
    65641 cccatctggc tgacggtcac ttttgctgtg gtgtgtgcca cgccggaaat gtctggcgcg
    65701 tagccgcgca gattgggagg caacatttga tcgtcagtgc tgacactgcc accaacataa
    65761 ttaaaaccgt cgaaaatatc ggaattgagg taatcttcgc ccagcgccag tttggctttt
    65821 aatgacggta acgcccgcca ggcataatag cgactccact cccatttttt ttgtgtgtca
    65881 tcgccgccaa attcatcgtc atcatcatta ctgcgcgtat gttgatagtt ggtctgccag
    65941 tcagcgcgca tacgccatgg ccccaggtta accccgaccg tcccgttgcc gctgatctcg
    66001 ttactgtcat cgccgccatt ttcttcgtgt cgtgtttgcg cagtgatgct gtagtccgca
    66061 atgatcccgg agatgccatc atcccaacga gaaggtggat cccagtcggg ccaggtatat
    66121 tcgaggtaag cctgcggtag tgaaatgact aatgcggact ggcttaaatc agccttaatt
    66181 tccacgcctt ccagttgacc gggcttcagg catttaccat cgtggctcca ttgcagattt
    66241 ttcgccacgt cttcttttaa accaaactgc gccaccagtt ccggtgtcag acaagcatag
    66301 cttttgctaa cgtcatcttc accagcatac cagtaaatat cgtactcttc cgccaatggc
    66361 tgtttattta gttgaacctg taaattgtat tttccgggct caacataccc ctggctggaa
    66421 aaacgcttca gatcaatttt tgtgtcgcct tttaattcca gaaaacggga atcaaactga
    66481 atgtcgtctt cagcccagac actgctataa ctccctgaca ttgccagcgc aatgcaccac
    66541 ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac acgtttttat ccgtttttat
    66601 tcttgttcgg tcaaaatcca tttaaatacc ccatagcagt gtcgctatgg gggtatgcct
    66661 tacaggtaag taatctggaa ggtggtattt gcttcgaaat tacccagatc tggcgcatca
    66721 gctgcgccga ccagccaggc tttaaagttc agagtctgct tcgctttgcc tttattagtg
    66781 gcagtatctt tcacgatttt ctggtcaata cccatgccgc ttttgtaaga ggtacccaga
    66841 gagtcaccga tcgccaggct aacattgtta aatgccgcac cggtatcggt attgaaaatg
    66901 gtgtaataat tgccgctatt tgcagaagaa acggtaccag taaaggtagt ggtcattgtt
    66961 tcctgagtat caaatacgca atcttgcagg cgaatctgga aatttttcgg cgtggtggtg
    67021 ccgttatttt tcagtttttc ggcaccgata tcacccatat caacttccag tttgtgatcg
    67081 tccggaacga tagaacacac tgagtcttca atggtgccgt aaaaacgtgc agtaccgttg
    67141 ttacccgcag cgattgttgc cgtagaggtg aacgcgccca tcaccattgc cgcagcaata
    67201 gctgttttaa caaacttact cattatttgt ccttaaacta taaataatta aaatataaac
    67261 attgcaatac attgattcag tcaatagcca atgtttatac ccagccattg aatacggcat
    67321 gcgatccaaa aaaacaggtc gtaaaaaatg tattcgttgg tgtggggtcg aatagtgatg
    67381 ttttcactcc cctgattcaa tttaatttta ctaatgagta aatgtagatt taattaatat
    67441 attgataggg gaaagattat cttaaggatc ttctaaaaaa acacgaaata tatatttaga
    67501 aaaaacagga agcatatcaa caatgaatgc caatagctta aaaaacaaac aatgaacata
    67561 taatgcgatt ggcattaacc cgcttttgtc gatataatat ataaacacta tagccagaat
    67621 ttaatcaagt ttcaattacg tcctgcctga ttattcaact ctattttctc cattatctgt
    67681 attaataaat gataatgagt gtcaaacaga cattattcat ttcaacatgt aactttgggc
    67741 aaaatcaaga tacgcgcaga agaatacttt ccgaatgaaa gttaaaggtg ttttatttat
    67801 agatggggag agtcttcgat agtagtgagg ttgacagata tccgctgcct gatgagcaat
    67861 tatgcccaat gaatctacct cattgggcat aatttggaac tcatcagata gcttttgcca
    67921 tcttcagatt acacgggctc atctgccagt tgaatgtgcc gtttccgctt tcatcaagcg
    67981 ttacgctggc aatggccgag gtggtaaaca taggcggcgt ttcgcccgga cataactcgg
    68041 caaccagata cccgactaac ggtaagtgag agatcaccag taccgaagca acaccttcat
    68101 tggtcaacgc ctgcaaatag gcactgacca gaccaacatc gccgcagggc gttaactccg
    68161 gcagaacttc tgcactggaa ggcaggttca gacaatcccc tacttcttcc agtgtttgct
    68221 cggctcgcag gaacgggctc accagaacgc gttcgatttc cactttttga cctttcagcc
    68281 agttcgccat caggcgagat tcgtcacaac cattagtggt cagaggacga acggaatcac
    68341 tggcggcatc gagggctgcg tcgccgtgac gcatgataaa aacttgcata ttgcaccgct
    68401 tttgttaacc agattcgccc gttttctttc atctgaaacc agagaaagaa aacggtggcc
    68461 ggcattgtgc cttatccatt caccgaatga aacgctgttt tttacctcaa tggcgtaagt
    68521 atagtcaata tgcgtttaca ttttgaccaa cactccgcca ttcagcgcgg attcatatag
    68581 ctttgacctt cttattgcag gtcagttgca gttgttttcc aaaaactttc cccacgcgcg
    68641 cccatctcga ccaaacgctc gcaaggggta aaacgggaac catactgcgt ggcaagtcgt
    68701 tgcattattg caaccacttc gcccgcgccg agagaatcga tatagcggaa cggtccaccg
    68761 agaaatggcg gaaaaccaat gccaaatacc gcgccaatat ccccgtcacg cacgctacgg
    68821 ataacctgct catcaacaca acgtactgct tcattcagca tcaacatcac acaccgttca
    68881 gcaacctgcg gtgcggagat tcgcccctgc ccttgtgtgc caatcagcgg gtaaatggcg
    68941 ggatcgacct gttttttgct tttacgccct ttctgaccat aaagatagaa accccggcca
    69001 ttttttctgc ctttgcgatc gtcgttcaaa attgaagaaa caacatttgc aggcgcgcta
    69061 aaacgttctc cataagcggc ttccagtaca ggaataattt tagtcccggt gtcgattcct
    69121 acctcatcca aaagttggat tgggcctacc ggaaaaccaa atttcactag cgcggcatca
    69181 atgtgctcta cccgttcacc ttgggtcaac atgcggatag cttcattaat gtaaggcgct
    69241 aagatgcgat tgacgtaaaa accggctttg tcacgcacga caattggcgt tttaccctgt
    69301 tttttcgcca gttttactgt ggtagcgatg gtttgcgccg atgtccccgc atgaggaata
    69361 atctccacca gcggcatttt ttccaccgga ctgaagaaat gcaggccgat aacttgctca
    69421 ggtcgcgtgg cgtgagcggc gatatcacca atcggtaaag atgacgtatt cgaagcaaag
    69481 atggtatgag cggcgcaatt ttgctcaact tccgccacca tctgttgttt caattcgaga
    69541 ttttcaaaca ccgcttcaat aatcagatcg cgatgggcaa agccgcgata gtccgtcgtt
    69601 ccggagatta atgccagctg tttgtcacgt tcgctggctt tgagatgacg acggcgaact
    69661 ttgccctcca gctgatccca actgtacttc agcgcatgat ttatgccctg cgggttgata
    69721 tctttaattc tgaccggaat ccccgcttta caagcagtga cataagcaat accgccgccc
    69781 atcaagccac cacctaaaat ccccacgctg tttaatggcg caggcggcgc atcactgccg
    69841 ggatctttct tcacgtccgt actggcaaaa aagatactac gcagcgcctg cgattgtggc
    69901 gtcatcgcca gttcgccaaa cgcccgagct tcggcgtcat aaccgctgct ggtgccctgc
    69961 gctaatcccg tttcaacaac ctccaggatg cgttctgtcg ccggataatt gccttgagtt
    70021 ttgtgttctg ttttcttgcc gaccattttg aacagcagcg cacgacctaa cggccccgcc
    70081 agaatacgct cgcgtacagg tagagggcgg gaagatgggc gctccttctt tgccagctca
    70141 acagcggctt ccagcagaat ggagtgcgga acaacgtcat ccaccagccc cagctttaat
    70201 gcctgtttcg cccgaagttg ttttccggtg aggatcatct ctaatgctgt gctgacgcct
    70261 atcagacgcg gtaaacgctg ggtgccgcct gaaccgggta acaatccaag ttgtacttca
    70321 ggcaaaccga gcaccgtttt aggatcgtca gtacaaacgc gaccgtggca cgccagcgcc
    70381 aactccagcc caccacccag gcaagcgcca tgaatagccg cgataacctg aatgggcaaa
    70441 gcatgaatct ccgccatcaa ctgttggccc tgccgcgcca gagcttccgc ttcttgcgcc
    70501 gttttgcagt tgccgatcat gttgatgtct gcgccagcaa tgaagttgtc cggtttagcg
    70561 gagacaaaca ccacgcctcg caactctttg ttttcacgga gttgcttaat aatggcgcgc
    70621 acctgcgagg caaactccgc cttcagggta ttcattttct cacccggtac gtcgatggtg
    70681 ataacggcaa tgttgtccag acgaacatta agggtaaacg ctgatgtcat ttccattatt
    70741 ccgcctccag aaccattgcc gcgccaagcc caccggcagc acaggcggta actaaaccaa
    70801 atccaccgcc gcgacggcga agttcatgca atgtctgggt aatcatccgc gcgccggtcg
    70861 ccgcgaaggg atgcccgtaa gcaatcgaac cgccaagcac gttaaatttg ctatcgtcca
    70921 cttcgccagt ggcatgtgca cgccccagtg cttcacgagc aaaacgttca ctacccagca
    70981 actgaatatt cgccagcgtc tgagctgcaa aggcttcgtg catatcgatc aatgtcagat
    71041 cgctcatcgt caaaccggca cgctccagcg ccagcggtgt tgaccaggct ggaccgagca
    71101 acatgtcctg ccagacatca atcgcagtaa atgcgtagct gcgcagatac cccagcggca
    71161 ccagccctaa ttctttcgcc cgggattcag tcatcaggat caccgctgcc gcgccatcgg
    71221 tcagcggcgt actgtttgcc gccgttaccg ttccgtgttt gcgatcaaac gccgggcgca
    71281 gctttgcgta atcggcaagc gaggaattac cgcgaatatt gttgtcttcg acaagcggtt
    71341 gtttataagg agggataaag gcagtcatca cctcttcttt gagttttccg tctgaccatg
    71401 cctgagcggc acgctgatgc gaacggtgcg ctaatgcatc ttgctgttct cgggtgatgc
    71461 cgtaggtttt cgccatttgc tctgcggtgt cgcccatccg caagccggta gaatattctg
    71521 ctaccgcagg tggtacgggc attaagtcgc gcaaacgcag gcgagagaag agtttcagtc
    71581 gctggctcat ggtacgagct ttgttgacat caaccagcac gcgcgccagt tttttactga
    71641 cgccaattgg caataccgaa gaggaatctg ccccaccggc aatccccgct cgaatagttc
    71701 ccgccatcag gctttctgcg acgtttgcaa ctgcctggaa actggtagcg caagcgcggc
    71761 tgacgctgta agcatcggta tgtacattca ttcccgtacc gagaacaatt tcacgcgcaa
    71821 tgttgggggc ttcaggcatt tgtacgacct gaccaaagac cagttgttca atcacttcgg
    71881 cggggatctc gctgcgtgcc agcagttcgc ctaccaccat cttccctaaa tcaaccgcgg
    71941 gaatgccatg aaaagccgtc gcctgacggg caaaaggcgt acgtaaaccg ctaacaatgg
    72001 cgatacgatc gccctggcgg gtaaccagcg gtaaaacctg acccataaca ctcccctgta
    72061 aaaaaataaa taaagtggtc tgacctgatc atagtcttaa ccattttttt acatttagcc
    72121 aagtggagaa aagggaaagt gggagctatg acacagagag aaaagaagaa gagaaaagaa
    72181 aacgcccctg ccagcaactg acaggggcgt acgtacccga gaggaattaa cgcagaccca
    72241 gctggaaaat cagcatttca gcttcgcagg caaaagtgaa atcgatatcc aggcgcacac
    72301 cgtcagactc ttcagtgaaa gtcggggtga ttttgcaagg ttcagattcc acgctacggg
    72361 ctttttcagt cagcgccgcc agcgtttgct ctgcttctgc gcggtttgca aacacgcggc
    72421 tgtaagacgc ggtgcagtcg gagttgtcca taatggtgcc aacatccata cagcagcaaa
    72481 ccggggtttc atcagcacta catttactca tcgttgattt cctctgtatg tgcacccaag
    72541 gtgccagata aacgttgtgg atattttacg cttccggaaa gtgctgctcc agttgttaat
    72601 tctgcaaaat cggataagtg accgaaatca cacttaaaaa tgatctaaaa caaaattcac
    72661 ccgaatccat gagtgcgcca cctccaaatt ttgccagctg gatcgcgttt cttagatcat
    72721 atttgaaaaa agatagaaac atacttgcaa cattccagct ggtccgacct atactctcgc
    72781 cactggtctg atttctaaga tgtacctcag accctacact tcgcgctcct gttacagcac
    72841 gtaacatagt ttgtataaaa ataaatcatt gaggttatgg tcatgagcca gaaaaccctg
    72901 tttacaaagt ctgctctcgc agtcgcagtg gcacttatct ccacccaggc ctggtcggca
    72961 ggctttcagt taaacgaatt ttcttcctct ggcctgggcc gggcttattc aggggaaggc
    73021 gcaattgccg atgatgcagg taacgtcagc cgtaaccccg cattgattac tatgtttgac
    73081 cgcccgacat tttctgcggg tgcggtttat attgacccgg atgtaaatat cagcggaacg
    73141 tctccatctg gtcgtagcct gaaagccgat aacatcgcgc ctacggcatg ggttccgaac
    73201 atgcactttg ttgcaccgat taacgaccaa tttggttggg gcgcttctat tacctctaac
    73261 tatggtctgg ctacagagtt taacgatact tatgcaggcg gctctgtcgg gggtacaacc
    73321 gaccttgaaa ccatgaacct gaacttaagc ggtgcgtatc gcttaaataa tgcatggagc
    73381 tttggtcttg gtttcaacgc cgtctacgct cgcgcgaaaa ttgaacgttt cgcaggcgat
    73441 ctggggcagt tggttgctgg ccaaattatg caatctcctg ctggccaaac tcagcaaggg
    73501 caagcattgg cagctaccgc caacggtatt gacagtaata ccaaaatcgc tcatctgaac
    73561 ggtaaccagt ggggctttgg ctggaacgcc ggaatcctgt atgaactgga taaaaataac
    73621 cgctatgcac tgacctaccg ttctgaagtg aaaattgact tcaaaggtaa ctacagcagc
    73681 gatcttaatc gtgcgtttaa taactacggt ttgccaattc ctaccgcgac aggtggcgca
    73741 acgcaatcgg gttatctgac gctgaacctg cctgaaatgt gggaagtgtc aggttataac
    73801 cgtgttgatc cacagtgggc gattcactat agcctggctt acaccagctg gagtcagttc
    73861 cagcagctga aagcgacctc aaccagtggc gacacgctgt tccagaaaca tgaaggcttt
    73921 aaagatgctt accgcatcgc gttgggtacc acttattact acgatgataa ctggaccttc
    73981 cgtaccggta tcgcctttga tgacagccca gttcctgcac agaatcgttc tatctccatt
    74041 ccggaccagg accgtttctg gctgagtgca ggtacgactt acgcatttaa taaagatgct
    74101 tcagtcgacg ttggtgtttc ttatatgcac ggtcagagcg tgaaaattaa cgaaggccca
    74161 taccagttcg agtctgaagg taaagcctgg ctgttcggta ctaactttaa ctacgcgttc
    74221 tgataacgcg ttcgcctgga taaagtcacc tgcatagcag gtgactttaa ctcccccact
    74281 tcaccgaaag tagtgtcctc attgcttacc tcctgagttt tgcaaacagc ctgttggcag
    74341 cttgcccttt caaatcaata agcggtatcc acagacaaac ctgaagggaa aggcatattt
    74401 tcaggcgttc tgctcgtcct tctcaaagag tttacttttc tgcatttcca ggatactccc
    74461 cccccctggc tattgtgcgc tcatacactc aaattaaaga taggttctaa ataaatgagc
    74521 gttttttgat agtctatttc attaggtaat atatatttgt aacaaatcaa tcaaaatgga
    74581 ataaaatcat gctaccatct atttcaatca acaataccag cgcagcttac ccagaatcca
    74641 tcaatgaaaa taacaatgat gaagttaatg gattagtaca agagttcaaa aaccttttta
    74701 atggtaagga aggaataagc acctgtatta aacatctact tgagcttata aaaaacgcca
    74761 tacgagtaaa cgacgatcct tatagattta atattaataa ttcctcagtt acttatattg
    74821 atattgactc caatgataca gaccatatta ctattggtat cgacaaccaa gaaccaatag
    74881 aattacctgc gaactataaa gacaaagaac tcgtccgtac tatcattaat gacaacatag
    74941 ttgagaagac tcatgatatc aataacaagg aaatgatctt cagcgcatta aaagaaatat
    75001 atgatggaga tcctggtttt atcttcgata agatatcaca caaactcaga catacggtaa
    75061 cggaatttga tgagagcggg aaaagcgaac caacggactt atttacctgg tacggtaaag
    75121 ataaaaaagg cgactctctc gctattgtaa ttaaaaataa aaacggaaat gattacttat
    75181 ctctcggtta ctacgatcag gacgactacc acattcaaag aggaattcgt attaatggtg
    75241 atagtctcac ccaatattgt agtgaaaacg ccaggagtgc ttcagcgtgg tttgaaagca
    75301 gtaaagctat catggcagaa tcatttgcaa ctggttccga tcatcaggtt gtaaacgagc
    75361 tcaacgggga aagactgaga gaaccaaacg acgtttttaa acgttatggt cgagcaataa
    75421 gatatgattt tcaagtggac gatgcaaaat ataaatgcga ccatctaaaa gaaatagttt
    75481 ctactttagt cggtaacaaa attaacgttg gccattctca aaaaatatat aagcatttta
    75541 aggatctcga aggtaaaatt gaagaaaggc ttcaaaatcg ccaggctgaa tatcaaaatg
    75601 aaattaatca accatctgcg ccaggtgtta atttt
//
LOCUS       DBBGLQ010000017        73227 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.017, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000017 DBBGLQ010000000
VERSION     DBBGLQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.017"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..1347)
                     /locus_tag="ACIYSB_001439"
     CDS             complement(<1..1347)
                     /locus_tag="ACIYSB_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428519.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWCVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSNRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSTITTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKVLACYQREANKEVALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINGLKTLHDTKIDSGTEGNDEINNIKINLSNILI
                     DSLDDAKINLEKVIDSMLETFFKLPYINDVKILEWCFNQSMQYSDDTTKIKHARSVID
                     HIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRLILR
                     LFDDVKLVFDKVPLSILVNDIFLKDFFMKQPDFAKWYFYQLLKSYEGEQMYLNELGY"
     gene            complement(1470..1922)
                     /gene="tabA"
                     /locus_tag="ACIYSB_001440"
     CDS             complement(1470..1922)
                     /gene="tabA"
                     /locus_tag="ACIYSB_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGC5428520.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTPETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLRGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(2067..2660)
                     /gene="bdcR"
                     /locus_tag="ACIYSB_001441"
     CDS             complement(2067..2660)
                     /gene="bdcR"
                     /locus_tag="ACIYSB_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGC5428521.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            2731..3444
                     /gene="bdcA"
                     /locus_tag="ACIYSB_001442"
     CDS             2731..3444
                     /gene="bdcA"
                     /locus_tag="ACIYSB_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGC5428522.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3575..3970
                     /gene="yjgH"
                     /locus_tag="ACIYSB_001443"
     CDS             3575..3970
                     /gene="yjgH"
                     /locus_tag="ACIYSB_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGC5428523.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            4251..4385
                     /gene="pyrL"
                     /locus_tag="ACIYSB_001444"
     CDS             4251..4385
                     /gene="pyrL"
                     /locus_tag="ACIYSB_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGC5428524.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4389..5324
                     /gene="pyrB"
                     /locus_tag="ACIYSB_001445"
     CDS             4389..5324
                     /gene="pyrB"
                     /locus_tag="ACIYSB_001445"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGC5428525.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRACDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5337..5798
                     /gene="pyrI"
                     /locus_tag="ACIYSB_001446"
     CDS             5337..5798
                     /gene="pyrI"
                     /locus_tag="ACIYSB_001446"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGC5428526.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5871..6257
                     /gene="ridA"
                     /locus_tag="ACIYSB_001447"
     CDS             5871..6257
                     /gene="ridA"
                     /locus_tag="ACIYSB_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGC5428527.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6463..9159)
                     /gene="mgtA"
                     /locus_tag="ACIYSB_001448"
     CDS             complement(6463..9159)
                     /gene="mgtA"
                     /locus_tag="ACIYSB_001448"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGC5428528.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGDISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(9300..9353)
                     /gene="mgtL"
                     /locus_tag="ACIYSB_001449"
     CDS             complement(9300..9353)
                     /gene="mgtL"
                     /locus_tag="ACIYSB_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGC5428529.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9535..10485
                     /gene="treR"
                     /locus_tag="ACIYSB_001450"
                     /pseudo
     CDS             9535..10485
                     /gene="treR"
                     /locus_tag="ACIYSB_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            10604..12025
                     /gene="treB"
                     /locus_tag="ACIYSB_001451"
     CDS             10604..12025
                     /gene="treB"
                     /locus_tag="ACIYSB_001451"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGC5428530.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            12075..13730
                     /gene="treC"
                     /locus_tag="ACIYSB_001452"
     CDS             12075..13730
                     /gene="treC"
                     /locus_tag="ACIYSB_001452"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGC5428531.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     MGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALAGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            14124..16262
                     /gene="nrdD"
                     /locus_tag="ACIYSB_001453"
     CDS             14124..16262
                     /gene="nrdD"
                     /locus_tag="ACIYSB_001453"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGC5428532.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16420..16884
                     /gene="nrdG"
                     /locus_tag="ACIYSB_001454"
     CDS             16420..16884
                     /gene="nrdG"
                     /locus_tag="ACIYSB_001454"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGC5428533.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPDKDIW
                     VWTGYKLDDLNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16929..17315)
                     /gene="cybC"
                     /locus_tag="ACIYSB_001455"
     CDS             complement(16929..17315)
                     /gene="cybC"
                     /locus_tag="ACIYSB_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGC5428534.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17498..18850)
                     /gene="pmbA"
                     /locus_tag="ACIYSB_001456"
     CDS             complement(17498..18850)
                     /gene="pmbA"
                     /locus_tag="ACIYSB_001456"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGC5428535.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLATMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18945..19496
                     /gene="yjgA"
                     /locus_tag="ACIYSB_001457"
     CDS             18945..19496
                     /gene="yjgA"
                     /locus_tag="ACIYSB_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGC5428536.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19652..21025)
                     /gene="mpl"
                     /locus_tag="ACIYSB_001458"
     CDS             complement(19652..21025)
                     /gene="mpl"
                     /locus_tag="ACIYSB_001458"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGC5428537.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21201..22199
                     /gene="fbp"
                     /locus_tag="ACIYSB_001459"
     CDS             21201..22199
                     /gene="fbp"
                     /locus_tag="ACIYSB_001459"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGC5428538.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGLFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22232..23227)
                     /gene="yjfF"
                     /locus_tag="ACIYSB_001460"
     CDS             complement(22232..23227)
                     /gene="yjfF"
                     /locus_tag="ACIYSB_001460"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGC5428539.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23214..24236)
                     /gene="ytfT"
                     /locus_tag="ACIYSB_001461"
     CDS             complement(23214..24236)
                     /gene="ytfT"
                     /locus_tag="ACIYSB_001461"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGC5428540.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(24250..25752)
                     /gene="ytfR"
                     /locus_tag="ACIYSB_001462"
     CDS             complement(24250..25752)
                     /gene="ytfR"
                     /locus_tag="ACIYSB_001462"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HGC5428541.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25892..26848)
                     /gene="ytfQ"
                     /locus_tag="ACIYSB_001463"
     CDS             complement(25892..26848)
                     /gene="ytfQ"
                     /locus_tag="ACIYSB_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGC5428542.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27158..27688
                     /gene="ppa"
                     /locus_tag="ACIYSB_001464"
     CDS             27158..27688
                     /gene="ppa"
                     /locus_tag="ACIYSB_001464"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGC5428543.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(28068..28409)
                     /gene="ytfP"
                     /locus_tag="ACIYSB_001465"
     CDS             complement(28068..28409)
                     /gene="ytfP"
                     /locus_tag="ACIYSB_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGC5428544.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28412..32191)
                     /gene="tamB"
                     /locus_tag="ACIYSB_001466"
     CDS             complement(28412..32191)
                     /gene="tamB"
                     /locus_tag="ACIYSB_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGC5428545.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDVGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVIQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32188..33921)
                     /gene="tamA"
                     /locus_tag="ACIYSB_001467"
     CDS             complement(32188..33921)
                     /gene="tamA"
                     /locus_tag="ACIYSB_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGC5428546.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34127..34765
                     /gene="msrA"
                     /locus_tag="ACIYSB_001468"
     CDS             34127..34765
                     /gene="msrA"
                     /locus_tag="ACIYSB_001468"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGC5428547.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35088..36431
                     /gene="paeA"
                     /locus_tag="ACIYSB_001469"
     CDS             35088..36431
                     /gene="paeA"
                     /locus_tag="ACIYSB_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGC5428548.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36493..36699)
                     /gene="ytfK"
                     /locus_tag="ACIYSB_001470"
     CDS             complement(36493..36699)
                     /gene="ytfK"
                     /locus_tag="ACIYSB_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGC5428549.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            37024..37581
                     /gene="ytfJ"
                     /locus_tag="ACIYSB_001471"
     CDS             37024..37581
                     /gene="ytfJ"
                     /locus_tag="ACIYSB_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGC5428550.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(37571..38311)
                     /gene="cysQ"
                     /locus_tag="ACIYSB_001472"
     CDS             complement(37571..38311)
                     /gene="cysQ"
                     /locus_tag="ACIYSB_001472"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGC5428551.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNIMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            38501..40444
                     /gene="cpdB"
                     /locus_tag="ACIYSB_001473"
     CDS             38501..40444
                     /gene="cpdB"
                     /locus_tag="ACIYSB_001473"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGC5428552.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYISAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNNSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPDNTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMVNANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(40567..40947)
                     /gene="ytfH"
                     /locus_tag="ACIYSB_001474"
     CDS             complement(40567..40947)
                     /gene="ytfH"
                     /locus_tag="ACIYSB_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGC5428553.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            41036..41896
                     /gene="qorB"
                     /locus_tag="ACIYSB_001475"
     CDS             41036..41896
                     /gene="qorB"
                     /locus_tag="ACIYSB_001475"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGC5428554.1"
                     /translation="MIAITGATGQLGHYVIKSLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSETDFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNK"
     gene            42005..42970
                     /gene="ytfF"
                     /locus_tag="ACIYSB_001476"
     CDS             42005..42970
                     /gene="ytfF"
                     /locus_tag="ACIYSB_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGC5428555.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            43078..43740
                     /gene="ytfE"
                     /locus_tag="ACIYSB_001477"
     CDS             43078..43740
                     /gene="ytfE"
                     /locus_tag="ACIYSB_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGC5428556.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43785..45197)
                     /gene="cycA"
                     /locus_tag="ACIYSB_001478"
                     /gene_synonym="dagA"
     CDS             complement(43785..45197)
                     /gene="cycA"
                     /locus_tag="ACIYSB_001478"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGC5428557.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFVHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLITPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(45506..46126)
                     /gene="fklB"
                     /locus_tag="ACIYSB_001479"
     CDS             complement(45506..46126)
                     /gene="fklB"
                     /locus_tag="ACIYSB_001479"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGC5428558.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLQGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            46345..46983
                     /gene="ytfB"
                     /locus_tag="ACIYSB_001480"
     CDS             46345..46983
                     /gene="ytfB"
                     /locus_tag="ACIYSB_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGC5428559.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46967..47614)
                     /locus_tag="ACIYSB_001481"
     CDS             complement(46967..47614)
                     /locus_tag="ACIYSB_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGC5428560.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            47827..48618
                     /locus_tag="ACIYSB_001482"
     CDS             47827..48618
                     /locus_tag="ACIYSB_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HGC5428561.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNLWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            48628..49470
                     /locus_tag="ACIYSB_001483"
     CDS             48628..49470
                     /locus_tag="ACIYSB_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGC5428562.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            49480..50256
                     /locus_tag="ACIYSB_001484"
     CDS             49480..50256
                     /locus_tag="ACIYSB_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HGC5428563.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            50266..51807
                     /locus_tag="ACIYSB_001485"
     CDS             50266..51807
                     /locus_tag="ACIYSB_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGC5428564.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDELVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDITPDGLHIAQSGQIPKLVTKVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMKDA"
     gene            51804..53885
                     /locus_tag="ACIYSB_001486"
     CDS             51804..53885
                     /locus_tag="ACIYSB_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HGC5428565.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELSEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            53906..54382
                     /locus_tag="ACIYSB_001487"
     CDS             53906..54382
                     /locus_tag="ACIYSB_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGC5428566.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGSGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            54425..55726
                     /locus_tag="ACIYSB_001488"
     CDS             54425..55726
                     /locus_tag="ACIYSB_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGC5428567.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            55779..56750
                     /locus_tag="ACIYSB_001489"
     CDS             55779..56750
                     /locus_tag="ACIYSB_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HGC5428568.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSQENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(56862..57311)
                     /gene="rplI"
                     /locus_tag="ACIYSB_001490"
     CDS             complement(56862..57311)
                     /gene="rplI"
                     /locus_tag="ACIYSB_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGC5428569.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(57353..57580)
                     /gene="rpsR"
                     /locus_tag="ACIYSB_001491"
     CDS             complement(57353..57580)
                     /gene="rpsR"
                     /locus_tag="ACIYSB_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGC5428570.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(57585..57899)
                     /gene="priB"
                     /locus_tag="ACIYSB_001492"
     CDS             complement(57585..57899)
                     /gene="priB"
                     /locus_tag="ACIYSB_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGC5428571.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(57906..58301)
                     /gene="rpsF"
                     /locus_tag="ACIYSB_001493"
     CDS             complement(57906..58301)
                     /gene="rpsF"
                     /locus_tag="ACIYSB_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGC5428572.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDADAGDSEE"
     gene            58629..58904
                     /gene="yjfY"
                     /locus_tag="ACIYSB_001494"
     CDS             58629..58904
                     /gene="yjfY"
                     /locus_tag="ACIYSB_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGC5428573.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(59033..59719)
                     /gene="ulaF"
                     /locus_tag="ACIYSB_001495"
     CDS             complement(59033..59719)
                     /gene="ulaF"
                     /locus_tag="ACIYSB_001495"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGC5428574.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(59719..60573)
                     /gene="ulaE"
                     /locus_tag="ACIYSB_001496"
     CDS             complement(59719..60573)
                     /gene="ulaE"
                     /locus_tag="ACIYSB_001496"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGC5428575.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(60583..61233)
                     /gene="ulaD"
                     /locus_tag="ACIYSB_001497"
     CDS             complement(60583..61233)
                     /gene="ulaD"
                     /locus_tag="ACIYSB_001497"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGC5428576.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(61247..61711)
                     /gene="ulaC"
                     /locus_tag="ACIYSB_001498"
     CDS             complement(61247..61711)
                     /gene="ulaC"
                     /locus_tag="ACIYSB_001498"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGC5428577.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(61721..62026)
                     /gene="ulaB"
                     /locus_tag="ACIYSB_001499"
     CDS             complement(61721..62026)
                     /gene="ulaB"
                     /locus_tag="ACIYSB_001499"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGC5428578.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(62042..63439)
                     /gene="ulaA"
                     /locus_tag="ACIYSB_001500"
     CDS             complement(62042..63439)
                     /gene="ulaA"
                     /locus_tag="ACIYSB_001500"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGC5428579.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            63794..64858
                     /gene="ulaG"
                     /locus_tag="ACIYSB_001501"
     CDS             63794..64858
                     /gene="ulaG"
                     /locus_tag="ACIYSB_001501"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGC5428580.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            64966..65721
                     /gene="ulaR"
                     /locus_tag="ACIYSB_001502"
     CDS             64966..65721
                     /gene="ulaR"
                     /locus_tag="ACIYSB_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGC5428581.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(65718..66467)
                     /gene="yjfP"
                     /locus_tag="ACIYSB_001503"
     CDS             complement(65718..66467)
                     /gene="yjfP"
                     /locus_tag="ACIYSB_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGC5428582.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            66649..66978
                     /gene="bsmA"
                     /locus_tag="ACIYSB_001504"
     CDS             66649..66978
                     /gene="bsmA"
                     /locus_tag="ACIYSB_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGC5428583.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            67127..67402
                     /gene="yjfN"
                     /locus_tag="ACIYSB_001505"
     CDS             67127..67402
                     /gene="yjfN"
                     /locus_tag="ACIYSB_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGC5428584.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(67519..69144)
                     /gene="aidB"
                     /locus_tag="ACIYSB_001506"
     CDS             complement(67519..69144)
                     /gene="aidB"
                     /locus_tag="ACIYSB_001506"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGC5428585.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(69228..70391)
                     /gene="yjfC"
                     /locus_tag="ACIYSB_001507"
     CDS             complement(69228..70391)
                     /gene="yjfC"
                     /locus_tag="ACIYSB_001507"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGC5428586.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPLYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(70394..71032)
                     /gene="yjfM"
                     /locus_tag="ACIYSB_001508"
     CDS             complement(70394..71032)
                     /gene="yjfM"
                     /locus_tag="ACIYSB_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGC5428587.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRSTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(71042..71440)
                     /gene="yjfL"
                     /locus_tag="ACIYSB_001509"
     CDS             complement(71042..71440)
                     /gene="yjfL"
                     /locus_tag="ACIYSB_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGC5428588.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(71458..72117)
                     /gene="yjfK"
                     /locus_tag="ACIYSB_001510"
     CDS             complement(71458..72117)
                     /gene="yjfK"
                     /locus_tag="ACIYSB_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGC5428589.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(72168..72866)
                     /gene="yjfJ"
                     /locus_tag="ACIYSB_001511"
     CDS             complement(72168..72866)
                     /gene="yjfJ"
                     /locus_tag="ACIYSB_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGC5428590.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVCMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(72885..>73227)
                     /locus_tag="ACIYSB_001512"
     CDS             complement(72885..>73227)
                     /locus_tag="ACIYSB_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGC5428591.1"
                     /translation="DVTNSENALIIKMNDYGDLQINILFTSRQMIIETFICPVSSISN
                     PDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNAL
                     DLAEITEEYSH"
BASE COUNT        18125 a        19221 c        18136 g        17745 t
ORIGIN      
        1 atatcctaac tcattaagat acatctgctc gccctcataa ctcttaagta attgatagaa
       61 gtaccactta gcaaaatcag gttgcttcat gaaaaaatcc ttcaaaaaaa tatcattcac
      121 taaaatgctg agaggcactt tatcaaaaac caatttaaca tcatcaaaaa gccttagtat
      181 taagcgcgat ttttcttgat tacttaaatt aaaatcattg acatatacaa ccaacttctc
      241 ccaaatcaat tcctgtaact cagggctgtt tttatagggt tctttattga ggttgaaaaa
      301 taatgtctca gctattttag actgatcgca gttaaaatca atatgatcga tcacggagcg
      361 ggcatgcttt atctttgtag tatcatcaga atattgcata ctttgattaa aacaccactc
      421 cagaattttt acatcattaa tatatggcaa tttaaaaaaa gtctccaaca tcgaatcgat
      481 gaccttttcg agattaattt ttgcatcatc taaggaatca ataagaatat tagacaggtt
      541 aattttgatg ttattaattt catcattacc ttctgtacca ctatcaattt tggtatcatg
      601 taacgttttc agaccattta tttcttcaag tataccacct tcgcctagta agtatggaat
      661 cgtgtcatca catacatgag gcattaatag cagatgttct ttctttattt tttctaatgt
      721 actggctaat ggcttttcta ataactcgtt ataatcaatt gcgtccttcc agttcaactg
      781 atgattattt aatagaagcg ccacttcctt gttagcttca cgttgataac acgccagaac
      841 cttgttagta atcaggtgta atgaactctc atcttttctt tcaaaggact ttccttcata
      901 acgattagtc gaaccatcag gttgttgaaa tataatagcc gtatgtcctt gacgagtccc
      961 catagtagtg atagtggaaa gtgtaatttc tatttccacc ttagataaat ccgctcgctc
     1021 cccttcagga ttattcataa caaacttcat tatctcactg ccatcattat ttttgatata
     1081 agatgaggac tcaatatgag ccatcatatt tcttcccgct tcgttggagc ggttgctaaa
     1141 atccacataa cccagggtaa atatttttac caaaaactct aaaaatgtct taggatcgag
     1201 gatatttctt gtattatcct gcccctcggc attacgagca aaattattca ttccaacaca
     1261 ccaggtattt aaaacttcac ttttttgttt aaaattgtcg gctaatgtaa tgtgttgaga
     1321 ataattcaaa tctgatattt tgctcatgac ggactccttg agttaataag aacgtctaag
     1381 cattacaaat tttactcaca aaaaaatatc aaaaaacgct cttttcacaa actcgccgta
     1441 aacctggtgt tatttacggc gaaaagtcat cacgccatca gcatcttcac tactgctttg
     1501 cgaacctggg ctggtgcgcc cactgcgcac agcggtttat gcacttcccc cggataaaac
     1561 acaacaaaat caccttcatt taggataact gttttctcat caacgccttc cggcaaaaat
     1621 gcgatgtctt tatcagctaa ccagtcggta tccggcgtgc ctgcaggttg cgtgctgaag
     1681 gtcatgcctt cctgacctct taacacaatc tgaatgtcga gatagcgggc atggtactcc
     1741 gcacggcgag cttcgtacgg ctcggtcata tcttccgaga taagataaaa cagacgattg
     1801 ccttcgatat cgtgcttacc ctttggcgtt tctggcgtaa cgtgcgcttt gatatgctca
     1861 atcgcctggc gtaactcctg gggtagccac ggctgaagat tatgaatatt tccaatgatc
     1921 atcttttcca ctctttaatt aaaacaatgt ttcatttcta tcgttatacg aaaagatgat
     1981 caattccgct agccttaacg cgtgaattat tgtcttatca caacttatcg gcaggcgcgt
     2041 tcccgccttt cgggtcaagg ccagcatcac tccttgagaa tagtttttat cgcttccccc
     2101 gccagtgcag ctgttgcaca gagttgttcg atggagtgcc cctcccgtgc cttcgcagaa
     2161 agccctgaca tcacggtact cataaaatca gtcacacatt gtgcgctttg tggatgccgc
     2221 ctggcgatat agtcataaat ggtcgtttct gcggcgtgat aatattgaac ggcaatatca
     2281 cgcgcttgtg gatcatgact atgaatacct tcaagaacca tacagccagc gcagccgccg
     2341 ttttggctat atctgcgcgc cgcttctttt aatacctcaa ccaggcactc gcctaccgga
     2401 cgatcatcac gaagaatatc ggcaagcgga atagcttccg taccgacata ttcattgagt
     2461 acacggctaa ataacccaac tttactgcca aaagctgcgt agaggctcgg ggggttaata
     2521 ccaagataat cagtaacttc agcaacactg acagcatcga aacctttttg gtgaaaaagc
     2581 acttttgccg cagagactgc ctgctcagga gcgaaacgtc tgggacgacc tggaacgcga
     2641 gattgttttt tagtgaccat tacaaaactt gttgacagaa agttaaaaca gttttgtaat
     2701 gcatgttaca taataaatca aggagtcctt atgggcgctt ttacaggtaa gacagttctc
     2761 atcctcggtg gcagtcgtgg aatcggtgcc gctatcgtac gtcgtttcgt cactgatggg
     2821 gccaatgtac gattcaccta tgcggggtcg aaagatgccg ctgaacatct ggcacaagag
     2881 actggagcga cagcagtatt tacagatagt gctgacagag acgctgtcat tgatgtcgtt
     2941 cgtaagagcg gcgcattgga tatcctggtg gtaaatgcag gtattggcgt ctttggcgat
     3001 gccctggaat taaatgccga cgatattgat cgccttttca aaatcaatat tcatgctcct
     3061 tatcatgcct ctgttgaagc cgcccggcag atgcccgaag gcgggcgcat cttaatcatc
     3121 ggctccgtga atggcgatcg tatgcctgtt gcaggcatgg ctgcttatgc cgccagcaaa
     3181 tctgccctgc aaggcatggc gcgcgggctg gcccgtgatt ttggaccgcg tgggatcacc
     3241 attaacgtcg tccagccagg gccaattgat accgacgcta atcccgccaa cgggccaatg
     3301 cgcgatatgt tgcatagttt gatggctatc aaaagacatg ggcaaccgga agaggtcgct
     3361 ggtatggtcg catggttagc agggccagaa gccagttttg ttaccggcgc gatgcatacc
     3421 attgatggcg cgtttggcgc ataaccgact acgctcaatt aagcccagcc attatccatg
     3481 atgtctgggt tttgtttact cacgtcgtcc gctaaaagcg gctcccggta aagagaaatc
     3541 ttctgttcat gtcgtaaatc ttgaggataa ccaaatggta gaaagaaccg ctgttttccc
     3601 tgctggccga cattcactgt atgctgagca tcgttattct gcggctattc gttccggcga
     3661 tttgctgttt gtttccgggc aagtaggaag tcgagaggac ggaacaccag aacccgattt
     3721 tcagcaacaa gtcagactgg catttgataa tttgcatgcg accctggctg ctgcgggatg
     3781 cacttttgac gatatcattg atgttacgag cttccatacc gatccagaaa accaatttga
     3841 agacatcatg acggtgaaaa atgaaatatt tagcgcccca ccttatccaa actggacggc
     3901 ggtgggtgtt acatggctgg caggctttga ttttgaaatt aaagtgatag cgcgcatccc
     3961 tgagcagtaa gcaagagtgt tagccgttcg ctttcacact ccgccctata agtcggatga
     4021 atggaataaa atgcatatct gattgcgtga aagtgaaaaa ggaaaaagca gggaatgtct
     4081 gcaattattg ataccgaagg acagttcccc tgcagaatca catcaaataa aaatgcatat
     4141 accttgactt ttaattcaaa taaaccgttt gcgctgacaa aatattgcat caaatgcttg
     4201 cgccgcttct gacgatgagt ataatgccgg acaatttgcc gggaggatgt atggttcagt
     4261 gtgttcgaca ttttgtctta ccgcgtctga aaaaagacgc tggcctgccg tttttcttcc
     4321 cgttgatcac ccattcccag cccctcaatc gaggggcttt tttttgccca ggcgtcagga
     4381 gataaaagat ggctaatccg ctatatcaga aacatatcat ttccataaac gaccttagtc
     4441 gcgatgacct taatctggtg ctggcgacag cggcgaaact gaaagcaaac ccgcaaccag
     4501 agctgttgaa gcacaaagtc attgccagct gtttcttcga agcctctacc cgtacccgcc
     4561 tctctttcga aacttccatg caccgcctgg gtgccagcgt ggtgggcttc tccgacagcg
     4621 ccaatacatc actgggtaaa aagggcgaaa cgctggccga taccatttcg gttatcagca
     4681 cttacgttga tgcgatagtg atgcgtcatc cgcaggaagg tgcggcgcgc ctggccaccg
     4741 aattttctgg caatgtaccg gtactgaatg ccggtgatgg ctccaaccaa catccgacgc
     4801 aaaccttgct ggacttattc accattcagg aaacccaggg gcgtctggac aatctccacg
     4861 tcgcaatggt tggtgacctg aaatatggcc gcaccgttca ctccctgact caggcgttag
     4921 cgaagttcga cggcaaccgt ttttacttca tcgcgccgga cgcgctggca atgccgcaat
     4981 acatccttga tatgctcgat gaaaaaggga tcgcctggag tctgcacagc tctattgaag
     5041 aagtgatggc ggaagtagac atcctgtaca tgacccgcgt gcaaaaagag cgtctggacc
     5101 cgtccgagta cgccaacgtg aaagcgcagt ttgttcttcg cgcctgcgat ctccacaacg
     5161 ccaaagccaa tatgaaagtg ctgcatccgc tgccgcgtgt tgatgagatt gcgacggatg
     5221 ttgataaaac gccacacgcc tggtacttcc agcaggcagg caacgggatt ttcgctcgcc
     5281 aggcgttact ggcactggtt ctgaatcgcg atctggtact gtaaggggaa atagagatga
     5341 cacacgataa taaattgcag gttgaagcta ttaaacgcgg cacggtaatt gaccatatcc
     5401 ccgcccagat cggttttaag ctgttgagtc tgttcaagct gaccgaaacg gatcagcgca
     5461 tcaccattgg tctgaacctg ccttctggcg agatgggccg caaagatctg atcaaaatcg
     5521 aaaatacctt tttgagtgaa gatcaggtag atcaactggc attgtatgcg ccgcaagcca
     5581 cggttaaccg tatcgacaac tatgaagtgg tgggtaaatc gcgccccagt ctgccggagc
     5641 gcatcgacaa tgtgctggtc tgcccgaaca gcaactgtat cagccatgcc gaaccggttt
     5701 cttccagctt tgccgtgcga aaacgcgcca atgacatcgc gctcaaatgc aaatactgtg
     5761 aaaaagagtt ttcccataat gtggtgctgg ccaattaatt gcggttggta ataaaagtct
     5821 ggctccctat aatgagccag actttttacc gctgtaataa aggagaaatc atgagcaaaa
     5881 ctatcgcgac ggaaaatgca ccggcagcta tcggtcctta cgtacagggc gttgatctgg
     5941 gcaatatgat catcacctcc ggccagatcc cggtaaatcc gaaaacgggc gaagtaccgg
     6001 cagacgtcgc tgcacaggca cgtcagtcgc tggataacgt aaaagcgatc gtcgaagccg
     6061 caggcctgaa agtgggcgac atcgttaaaa ccaccgtgtt tgtgaaagat ctgaacgact
     6121 tcgcaaccgt taatgctacc tacgaagcct tcttcaccga acacaatgcc accttcccgg
     6181 cacgttcttg cgttgaagtt gcccgtctgc cgaaagacgt aaaaattgag atcgaagcga
     6241 tcgctgttcg tcgctaatct tgatggaaat ccgggctatc atgcccggat taaatctgat
     6301 gacaaacgca aaattgcctg atgcgctacg cttatcaggc ctacgtgatt cctgcaattg
     6361 attgaatttg taggccggat aaggcattta cgccgcatcc ggcatgaaca acgctcactt
     6421 tgtctgcaat ctgaatcggg gctatcgtgc ccagtttatt ctttactgcc agccgtaacg
     6481 acggctatag aaccctttca ccaactgggt taacgtcata taccctgcca gaatcgcaac
     6541 cagccacggg aaatagctta acggcagcgc ctgcagctgc agataactgg ccagcggtga
     6601 aaacggcaat gcgatcccga caatcatcac gatcacggtc atgatcatta acggccacga
     6661 tgcacagctc tgaataaacg gcacacggcg ggtacggatc atatgcacaa tcagcgtttg
     6721 cgacagtaag cccaccacaa accatcccga ctggaacagc gtttgcgttt ccggcgtgtt
     6781 ggcatggaat acccaccaca tcaggcaaaa cgtcagaata tcgaagatcg agctgatcgg
     6841 tccgaagaag atcataaagc gccccagatc cgccggattc caacgctgcg gcttttgaat
     6901 ttgctcgtcg tcgacgttat caaacgggat cgccacctgt gacacatcgt acagcaggtt
     6961 ctgaataagc aagtgtaacg gcaacatcgg caggaagggc aagaaagcac tcgccaccag
     7021 cacgctgaac acattaccga agttagagct cgccgtcatt ttgatgtatt tcagcatgtt
     7081 ggcgaaagtg cgacgtccct caataacccc ctcttccagc accatcaggc ttttttccag
     7141 caggatgata tcagccgctt cacgggcgat atctaccgcg ccgtccacag aaatgccgat
     7201 atccgcagcg cgtaaggcgg gcgcatcatt aataccatcg cccataaagc caaccacatg
     7261 cccttcgcgc ttcagtaagg tcacaatgcg ttctttatgc atcggcgtca gacgagcaaa
     7321 cagcgtggtg cgctgtgcga gattagccag ttcgtcgtca gatagcgttt caatatcact
     7381 accaatgacc acctctcccg cgtccagccc cacttcatgg cacactttcg ctgcaactaa
     7441 ctcactgtcg ccagtaagga tttttacggt aattccactc gcttttaatg ccttcagtgc
     7501 cggagcagtt gtctcttttg gcggatcaag gaaagcaata tatccttcga ggatcaggtc
     7561 ggattcatcc gcccgctggt aatctccttc acgcgctggc aggtatttcg tcgccaccgc
     7621 aaccacgcgc agcccctgac gattcagcgt atcagtaacc cgcttaatct tacgcagcat
     7681 gatgtcatcg agcggcacaa tctcgccatt gtgacgcacc tgcgaacaca cattgaggat
     7741 ttcctgcaat gcacctttgc aaaccagctg atggtgttcg gtattttctg ccactaccac
     7801 cgacatccga cgacgctcga aatcaaacgg aatctcatca attttttgcc aacggctggc
     7861 cagcgagcgc gctgactctt catccgtacc ttcgagcacc gctgtatcga gcaggttttt
     7921 aagtccggtc tgataatgac tgttcaacca cgcgctatgc agcacgcgtt cgctggtttt
     7981 accggagata tcggtatgat tctccagcac aattttgtcc tgtgtcaggg tgccggtttt
     8041 atcagtgcac agaatatcca ttgcgccaaa gttctgaata gcatccagat gtttgacgat
     8101 cactttctgt ttcgacagct ttactgcgcc gcgcgccagc gtcgaggtaa caatcatcgg
     8161 caacatttcc ggcgttaggc ctaccgcgac cgaaagcgca aacagcgccg cttcccacca
     8221 gtcgcctttg gtgtagccat tgattaacag caccaccggt gccatcacca gcataaagcg
     8281 aatcagcagc atactgacgc ggctgatccc ttgctgaaag gcattcggct cgctttcctg
     8341 ctcactaaca cgccccgcca gttgaccaaa ccaggtattg gcacctgtag caatcaccat
     8401 tgcttgtgcc gtgccgctca ccacggtggt gcccataaaa cacagggtgt cgcactccag
     8461 cggattgctg tgctccggct ggcgagtggt tgcggctttt tctacgggca gagactcacc
     8521 ggttaacgac gcctgagcga cgaacagatc ccgcgcctgc aagatacgta aatctgccgg
     8581 gatcatatct cccgccgcca gttttataat atcgccgggc accagctggt cgatcgggat
     8641 ctccagccag ccattttcgc ctttgtcgtt aattacgcgc agcaccgtcg cagtattgct
     8701 gaccatcgct ttcagggcat ctgccgcttt agtggaacgt gcttcctgaa taaagttcag
     8761 caacgtagaa atggcgacca ttagcgcgat aacgcccgcg gcaaataaat cttccgtggc
     8821 gtaagaaata tcgccgagaa tggtgagtaa aatattaaag gggttgcgat agcagaccca
     8881 taaatgtacc caccacggcg acggttgttg tgcgggtaat ttattttcac catgttgttc
     8941 gcgggcagat tccacttccg cctgatttaa cccttccgga tgggtgtcga acgttttcca
     9001 caattcttct tcgggcatca ccgccatttt caggcaatgc gcacttaagg acggcgggac
     9061 caccgtattc actgtctgct gcgcgccagg caatggatca cgatgaacca gacgggaagg
     9121 taaatggcga atgagccggg taaaaatttc tttaaacata aggagtccct ccgcactgtc
     9181 tgaaacagta cagaaatgtc ttcaggcgtg gcgaagcctt acctgaaggt aataaagctg
     9241 agcagggacg ttaaaacgtc acggccttac gcatccggta acgccgtgaa aagacgggct
     9301 taccgaaaaa gttttaatct ccgtcgaggg agaggcgtgg gatcaggttc catataacct
     9361 ccggtaagtg aaaaaagcgc cgcaaataat aaggagaatc caataatatt cgtagcattt
     9421 atggcgatat aatacctgct ggcaactaaa ccaacgataa accagacttt acccattgct
     9481 gaatgcacgg gtaaagttag cctcaaataa ttaaacaaca cgttacggga caacaggatg
     9541 caaaatcggc tgaccatcaa agacatcgcg cgcttaagcg gcgtggggaa atctacggtt
     9601 tcccgggtgc tgaataacga aagcggcgtg agccagcgca cgcgcgagcg cgtggaagca
     9661 gtgatgaatc agcatggatt ttccccttcc cgctctgcgc gcgctatgcg tgggcaaagc
     9721 gataaagtgg tcgccatcat tgttacccgc ctggattcgt tgtcagaaaa tctcgccgtt
     9781 caaaccatgc tgccagcgtt ctatgaacaa ggttacgacc caatcatgat ggaaagtcag
     9841 ttttccccgc aattagttgc cgaacatttg gggatcctaa aacggcgtaa tatcgacggc
     9901 gtagtgctgt tcggttttac cggcataaca gaagaaatgt tagcccactg gcagtcatcg
     9961 ctggttctgc tggcgcgtga cgcaaaaggc tttgcttcgg tctgttatga cgacgaaggg
    10021 gcaatcaaga tcctgatgca acggctctat gaccaggggc atcgtaatat cagttatctc
    10081 ggcgtgccgc acagtgacgt gacgaccggt aagcgacgtc acgaagccta cctggcgttc
    10141 tgcaaagcgc ataaactgca tcccgttgcc gctctgccag ggcttgctat gaagcaaggc
    10201 tatgagaacg tagcaaaagt gattacgcct gaaactaccg ccttactgtg cgcaaccgac
    10261 acgctggcac ttggcgcaag taaatacctg caagagcaac gcatcgacac cttgcaactg
    10321 gcgagcgtcg gtaatacgcc gttaatgaaa ttccttcatc cggagatcgt caccgtagat
    10381 cctggttacg ccgaagctgg acgccaggcg gcctgccaat tgatcgcaca ggtaaccggg
    10441 cgcagcgaac cgcaacaaat catcatcccc gccaccctgt cctgatcgtt tcctgaacga
    10501 taaattgtga tcttcgctgc gtttcgggaa cgttcccgtt tttaaatttt tccgcgcaat
    10561 atattctgca gccaaccaaa aatgtcatct gccatggggc tttatgatga gcaaaataaa
    10621 ccaaacggat atcgatcggt tgattgaact ggtcggcggg cgcggcaata ttgcgacggt
    10681 gagccactgt attactcgcc tgcgctttgt cctcaaccaa ccggccaatg ccagaccgaa
    10741 agaaattgag caactcccca tggtgaaagg ctgtttcacc aatgccgggc aatttcaggt
    10801 ggtgattggc accaatgtgg gtgattacta tcaagcactg attgcgtcaa ccggacaggc
    10861 gcaggttgat aaagagcagg taaaaaaagc cgcccggcag aatatgaaat ggcatgagca
    10921 gttgatctct catttcgcgg agatcttctt cccgttgctg cccgcgttga ttagcggcgg
    10981 tttgatcctc ggttttcgca atgtgatcgg cgatttgccc atgagcaacg gtcaaacgct
    11041 ggcgcaaatg tacccttccc tgcaaacgat ctacgatttt ctgtggttga tcggtgaagc
    11101 gatcttcttc tacctgccgg tcgggatttg ctggtcagcg gtgaaaaaaa tgggcggcac
    11161 gccgatcctt ggtatcgtac ttggcgtaac gctggtttct ccacagctga tgaacgctta
    11221 tctgctcggg cagcagctgc cggaagtgtg ggactttggc atgttcagca tcgccaaagt
    11281 aggctatcag gcgcaggtga tcccggcact gttagccggg ctggcgctgg gcgttattga
    11341 aactcgcctt aaacgcatcg tgccggatta cctctatctg gtggtggtgc ccgtctgttc
    11401 gctgatcctc gcggtgttcc tcgcccatgc gctgattggt ccgtttggac gcatgattgg
    11461 cgatggcgtt gcctttgcgg tacgtcacct gatgaccggc agctttgcac ctattggcgc
    11521 agcattgttt ggcttcctgt acgccccgct ggtgatcacc ggtgtacacc agaccacgct
    11581 tgctattgat ttgcagatga ttcaaagcat gggtggtacg ccagtgtggc cgctgattgc
    11641 gctgtcgaat atcgctcagg gctccgccgt gataggcatt atcatttcca gccgcaagca
    11701 caatgaacgc gagatctccg tgcctgccgc tatctccgcc tggcttgggg tcactgagcc
    11761 tgcaatgtac ggcatcaacc tgaaatatcg cttcccgatg ctgtgcgcga tgattggttc
    11821 tggtctggca ggattgctat gcggcctgaa cggcgttatg gcgaatggca tcggcgtagg
    11881 cggcctgccg ggaattctct cgattcaacc gagctactgg caggtgtttg cgctggcaat
    11941 ggctatcgcc atcatcatcc cgattgtact cacctcgttt atctatcagc ggaaataccg
    12001 cctgggcacg ctggacattg tttaattttc ttcggggcgc aattgcgccc cctcgcattc
    12061 gcaggaataa cgtaatgact aatcttcccc actggtggca aaacggcgtt atctaccaga
    12121 tttatccaaa gagttttcag gacaccacgg gtagcggtac cggcgattta cgtggcgtta
    12181 tccaacgcct ggactatctg cataaaatgg gcgttgatgc catctggcta accccctttt
    12241 atgtctctcc ccaggtcgat aacggttacg acgtagcgaa ctatacggcg attgatccca
    12301 cctacggcac gctggacgat tttgacgaac tggtgacgca ggcaaaatcg cgcgggattc
    12361 gtatcattct cgatatggtg tttaaccata cctctaccca acatgcctgg tttcgcgagg
    12421 cgctgaacaa agaaagccct taccgccagt tttatatctg gcgcgatggc gaaccagaaa
    12481 cgccaccgaa taactggcgt tcaaaatttg gcggtagtgc gtggcgctgg catgcggaaa
    12541 gcgaacagta ctatttgcat ctctttgcac cagaacaggc ggatctcaac tgggagaatc
    12601 cagcggtacg cgcagagctg aaaaaagtct gtgaattctg ggccgatcgt ggggtcgatg
    12661 ggttgcgcct ggatgtggtg aatctgatct ccaaagaccc gcgtttccct gaagacctgg
    12721 atggcgacgg gcgtcgcttc tacaccgacg ggccacgagc acacgagttt ttgcacgaga
    12781 tgaaccgcga tgtgtttacg ccacgcgggt taatgaccgt aggtgaaatg tcctccacca
    12841 gccttgagca ttgccagcga tacgcggcac tggcaggcag tgaattatcg atgaccttta
    12901 attttcatca cctgaaggtc gattatcccg gtggtgaaaa atggacgctg gctaaacctg
    12961 actttgtggc gttgaaaaca ttgttccgcc actggcaaca aggaatgcac aacgtagcat
    13021 ggaatgcctt gttctggtgt aaccacgatc agccgcgcat tgtttctcgc tttggtgatg
    13081 aaggtgaata ccgcgtgcct gcggcaaaaa tgctagcgat ggtgctgcat ggcatgcagg
    13141 gaacgccgta tatctaccag ggcgaagaga ttggcatgac caacccgcat ttcacgcgca
    13201 ttactgacta tcgcgacgtg gagagcctca atatgtttgc cgagctgcgc aacgatgggc
    13261 gtgatgccga cgagttattg gcaatcctcg ccagtaaatc ccgtgacaac agccgcacgc
    13321 ccatgcaatg gagcaacggc gataatgccg ggtttacggc tggcgaaccg tggattggcc
    13381 tgggcgataa ctatcaacaa atcaacgtag aagccgcgct ggccgatgat tcctcggtgt
    13441 tttacaccta ccaaaagtta atcgcactgc gtaagcagga agccatcctg acatggggca
    13501 attaccagga tctgctgcca aacagccctg tattgtggtg ctatcgccgt gaatggaagg
    13561 ggcaaacctt gctggtcatt gccaacctta gccgtgagat ccaaccctgg cagccagggc
    13621 aaatgcgcgg caactggcag cttgtgatgc ataactacga agaagcctca ccacaaccct
    13681 gtgccatgaa tttacggcct tttgaggctg tctggtggtt acagaagtaa atcttcccta
    13741 agccccggta atgccggtca ttccggggtt ttgtctgtca gcatgttgtt tttgttgatt
    13801 tttcaaccag caaattcatt aaaaaattta cataacgctg tagcgcccgt catccgtacg
    13861 ctctgctttt tactttgagc tacatcaaaa aaagttcaaa catccttgat gcaaagcact
    13921 atatatagac tttaaaatgc gtcccaaccc aatatgttgt attaatcgac tataattgct
    13981 actacagctc cccacgaaaa aggtgcggcg ttgtggataa gcggatggcg attgcggaaa
    14041 gcaccggaaa acgaaacgaa aaaaccggaa aacgcctttc ccaatttctg tggataacct
    14101 gttcttaaaa atatggagcg atcatgacac cgcatgtgat gaaacgagac ggctgcaaag
    14161 tgccgtttaa atcagagcgc atcaaagaag cgattctgcg tgcagctaaa gcagcggaag
    14221 tcgatgatgc cgattattgc gccactgttg ccgcggttgt cagcgagcag atgcagggcc
    14281 gcaaccaggt ggatatcaat gagatccaga ccgcagttga aaaccagctg atgtcgggtc
    14341 catacaaaca actggctcgt gcttacatcg agtaccgtca cgatcgcgac attgaacgtg
    14401 aaaaacgcgg tcgcctgaac caggagatcc gtggtctggt cgagcagacc aacgcctcgt
    14461 tactcaacga aaacgccaac aaagacagca aggtgattcc aacccagcgc gacctgctgg
    14521 ccgggatcgt ggctaaacac tatgcacgtc agcacctgct gccgcgtgac gtggtgcagg
    14581 cacatgagcg tggcgatatt cactatcacg atctcgatta ctcaccgttt ttcccgatgt
    14641 tcaactgcat gttgatcgac ctgaaaggca tgctgaccca gggctttaaa atggggaacg
    14701 ccgagattga accgccgaag tcgatctcaa cggcaaccgc ggtaactgcg cagattattg
    14761 ctcaggttgc cagccatatt tatggcggca ccaccattaa ccgtatcgat gaagtgctgg
    14821 caccgtttgt caccgccagc tacaacaaac atcgcaagac cgcagaagag tggagcatcc
    14881 cggacgcaga aggctacgct aactctcgta ccattaaaga gtgctacgat gccttccagt
    14941 cgctggagta cgaagtaaac accctgcata ccgccaacgg tcaaacgccg tttgtaactt
    15001 ttggttttgg tctgggcacc agctgggaat cgcgcctgat tcaggaatct atcctgcgta
    15061 accgtatcgc aggtctcggt aaaaaccgta aaactgcggt gttcccgaaa ctggtgtttg
    15121 cgattcgcga tggtctgaac cataaaaaag gcgatccgaa ctacgacatc aaacagctgg
    15181 cgctggagtg cgcaagcaag cgcatgtatc cggatatcct gaactacgat caggtagtga
    15241 aagtcaccgg ttcgtttaaa accccgatgg gctgccgcag cttcctcggc gtgtgggaaa
    15301 atgaaaacgg cgagcagatc cacgatggtc gtaacaacct cggcgtgatc agcctgaacc
    15361 tgccgcgtat tgctctggaa gcaaaaggcg atgaagccac cttctggaag ctgctggatg
    15421 aacgtctggt gctggcacgt aaggcgctga tgacccgtat cgctcgtctc gaaggcgtga
    15481 aagcgcgcgt ggccccgatc ctctatatgg aaggtgcttg tggcgtgcgt ctcaatgctg
    15541 acgatgatgt ttctgaaatc ttcaaaaacg gtcgtgcgtc tatttcgctg ggttacatcg
    15601 gcatccacga aaccattaac gcgctgttcg gcggcgagca tgtttacgac aacgagcagc
    15661 ttcgcgcgaa aggtatcgcc attgttgaac gtctgcgtca ggcagtggat cagtggaaag
    15721 aagaaacggg ttatggtttc agtctctaca gcacgccgag tgaaaacctg tgcgaccgct
    15781 tctgccgtct cgatactgct gagtttggcg tggtgccggg cgtgaccgat aaaggttact
    15841 acaccaacag tttccacctc gatgtggaga agaaggtgaa cccgtacgac aagatcgact
    15901 ttgaagcgcc ttacccgccg ctggcgaacg gtggtttcat ttgctacggc gagtatccaa
    15961 acattcagca caacctgaag gcgctggaag atgtctggga ttacagctat cagcatgtac
    16021 cgtattacgg caccaataca ccgattgatg agtgctacga gtgtggcttt accggtgagt
    16081 tcgagtgcac cagcaaaggc ttcacttgcc cgaaatgtgg taaccatgac gcctcccgtg
    16141 tgtcggtaac tcgccgcgtg tgcggatatt taggtagccc ggatgcacgt ccatttaacg
    16201 ccggtaagca ggaagaagtt aagcgccgcg ttaaacattt gggaaatggg cagataggtt
    16261 aatcttcact ctacgttaag caaatgccgg atgcggcgta aacgccttat ccggcctaca
    16321 tttggcaact gctccaggcc tgataagacg cgacagcgtc gcatcaggca ttgattaccg
    16381 gatgcagcct acacgcggac tgataagcca ggcgaaataa tgaattatca tcagtactat
    16441 cctgtcgaca tcgtcaacgg ccccggcact cgctgcaccc tgtttgtctc cgggtgtgtt
    16501 catgaatgcc ccggttgcta taacaaaagc acatggcggg taaattctgg tcagccattt
    16561 accaaagcaa tggaagacca gatcattaac gatctgaatg acactcgtat caaacgccag
    16621 gggatttccc tctccggcgg cgatccgctg catccgcaaa acgtgccgga tattctgaaa
    16681 ctggtaaaac gcatccgcgc cgagtgtccg gataaagaca tctgggtgtg gacaggctat
    16741 aaactcgacg atctcaacgc tgcgcaaatg caggttgttg atttgattaa cgtactggtc
    16801 gacggcaaat ttgtgcagga tttaaaagac ccttccctga tctggcgcgg cagcagcaat
    16861 caggtggtgc atcatttgcg atgataagtt ccctgttacc tgagtgcagg caacagggaa
    16921 atgaggaatt aacgatactt ctggtgatag gcgttgcggg tcgttttcag ttgctctgcg
    16981 gcagcctgcg cttcttttac tttaccttca tttgccagct tcagcgcgtc gtcaatctga
    17041 ccaaccagaa tatcgaaacc gtgacggaag tctttcattt ccgggctgtc cggtgattta
    17101 tcttcgagct tcggcggcgt tgctttttgc gcatccagcg ctgcggcgcg catcttcgtt
    17161 aacgcgtctt tgacttgcgc cgcgttatcg gctttttcga ccacttttaa agtgtcgttg
    17221 agggtttcca tattgtcttc aagatcagcg gcaaacgacg ccgaactgaa taccaacgag
    17281 gagactgcaa gaatagctaa caggctttta cgcattgctc acttcctttt ttatttacac
    17341 ttcatccttc acactgcctc atcgacggct acgctcattc gcccgggtca cgtactgcga
    17401 gtacgctccc cggccctcat tcactttccg tctcgatgca gtgcgaatga ttttgtgtaa
    17461 ttttttagtt tatagcgcgg caggtcgcgc cagtttttta ctgcccggcg attttcatct
    17521 ccggcaacag cacagaacca cactgtatat tactgcgtgt ttcaatatcg ttaccgacgg
    17581 tgacaatatt gcgccacata tcttttaaat tacctgcgat ggtgatttcg ctaaccggat
    17641 actgaatttc gccattctct acccagaagc ccgctgcacc acgggaataa tcaccggtaa
    17701 tggcactcac gccctggccc atcagctccg tcaccaccag cccggtaccc atctctttga
    17761 gcatttgttc gaagcttagc ccttgtccgg caatccgcca gttgtgaata ccgcccgcat
    17821 gtccggtgct tttcagcccc agtttccgcg ccgagtagct ggtcagcagc cactgagtca
    17881 ggatgccatc tttaataata tcgcgacgct cggtgcgcac accttcgctg tcaaatggcg
    17941 tcgatgccag ccctttcagc agatgcggat gctcttcaat ggtcagccag tccggcagaa
    18001 tttgtttacc cagcgaatcc agcaggaagg tggatttacg gtaaaccgat ccacccgcta
    18061 tcgcccccac cagatggcca aaaagcccgg ttgccacttc attggcaaaa atgaccggcg
    18121 ctttcatggt ggcgagttta cgcggtgaca ggcgcgataa agtacggcga gcacagtcgg
    18181 ccccaaccca ctctggcgtt tgcagatcgc tcatcgcacg accaatggtg taggcgtaat
    18241 cacgctccat atcgccattt tcttcggcaa ttacacagct ggagagcgaa tgacgcgttg
    18301 agcagtaacc ctgcaacatg ccgtggctgt tgccaaaaac tttgacaccg tagtggctgt
    18361 taaagctgcc accttcggta ttggtgatgc gtttgtccgc ctgcaatgcc gcctgttctg
    18421 cgcgggccgc cagttcaatg gcttcatccg gggaaacttc cgcagggtgg aacaagtcga
    18481 gatctggtgc gtcaaaggcc agcagctctt tgtctgccac gccggcacag ggatctggcg
    18541 aggtataacg ggcaatatcc agcgccgcct gtacagtacg ggcaatggcc tgcgggctta
    18601 aatcggtgga tgatgcgcta cctttgcggt tctggtgata aacagtgatc cccagcgcgc
    18661 catcgctatt gaattcaaca ttctccactt caccataacg cgtgcttacg ctaatgccgg
    18721 tggtcttgct gacggcaact tccgcaccgt ccgatttgcc tgaggccaac tccagcgcag
    18781 tggaaactgc ttcttccaga atcttgcgct gcgcttcaac ttgagagatt actttcattg
    18841 caagtgccat aatgtagaga gtttctctga agtctaacag agaaccgttt ttcagtgcgc
    18901 atcttaactg gtaacattag cctctttttt ttaaggagcc tgagatgact aagcagcccg
    18961 aagactggct cgacgacgtt cccggtgatg acatcgaaga cgaagacgat gaaattatct
    19021 gggtcagtaa aagtgaaatt aaacgtgatg ccgaggagct aaaacgcctt ggcgcggaaa
    19081 ttgttgatct ggggaaaaac gcgctggata agatcccgtt agatgcggat ctgcgcgcgg
    19141 ctattgagct cgcccagcgt attaagatgg aaggtcgccg ccgccagttg cagctcatcg
    19201 gtaagatgct gcgccagcgc gacgtagagc ctattcgtca ggcgctggat aagctgaaaa
    19261 accgtcacaa ccagcaggtg gtgctgttcc ataaactgga aaacctgcgc gatcgtctga
    19321 tcgatcaagg tgatgacgcc atcgctgagg tattaaatct gtggcccgat gccgatcgtc
    19381 agcaactgcg tactctgatc cgcaacgcga agaaagagaa agaagggaat aagccgccga
    19441 aatccgcacg ccagattttc cagtatctgc gcgaactggc ggagaatgaa gggtaatttt
    19501 cgttgttgat gtgagtatgg tttgccggat gcggtgcacc tgtaggcctg ataagacgca
    19561 tcaagcgtcg catcaggcac tatgctcaaa tgccggatgc ggcgtgaacg ccttatccgg
    19621 cctacgcaca ctatatcagg ctaaggccga attactgcgc ggcttccgcc ttcttcgcca
    19681 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    19741 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    19801 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    19861 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    19921 tccccatttt catggtattc gagcgcggtt ccagcacggc aataatgcgc gccgtaccgc
    19981 caactttgcc acgcagcgcc gcaagcgttg ccagaatcgc cgttgggtga tgggcaaaat
    20041 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcgttaa
    20101 taaacgaacc cagcgcgtta gcggcatctg ccggcgctac accaacatgg cgagccgccg
    20161 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    20221 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    20281 cctgccagtg cccctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    20341 tctgtttcag gttgatgtcg ttttctggcc agataatacg gccctgcccc ggaacgatac
    20401 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    20461 cgaactcaag gttgttgagg atcagcgtac gtgggcagta atggacaaat ttagagcgtt
    20521 tgtcgaagaa ggcgcagtca tactcatccg cttcgataac aaagaagtcg ctttcgccca
    20581 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    20641 cgcactgttc cagaatccag gttgccattc ccgcggtggt ggttttgcca tgtgtaccgg
    20701 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    20761 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    20821 tgccaataat caccagatcc ggctgcggat cgagctggct ggcatcgtaa ccctgaatca
    20881 gttcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    20941 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aacgtgccac
    21001 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    21061 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    21121 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    21181 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    21241 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    21301 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    21361 ctgagaacgt gcagggcgag gttcagcaga aactcgactt gttcgctaat gaaaaactga
    21421 aagccgcact gaaagcacgc gatatcgttg cgggcattgc ctctgaagaa gaagatgaga
    21481 ttgtcgtctt tgaaggctgt gaacacgcaa aatacgtggt gctgatggac ccactggatg
    21541 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    21601 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    21661 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    21721 gtgttcacgc ctttacttac gatccttcgc tgggcctttt ctgtctgtgc caggaacgga
    21781 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    21841 cgaacggggt gaagaagtac attaaattct gccaggaaga agataagtcc accaaccgcc
    21901 cttatacctc acgttatatc ggttcactgg tcgcggattt ccaccgtaac ctgctgaaag
    21961 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgctgt
    22021 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    22081 aagagcgtat tctggatatc atcccggaaa ctctgcacca gcgccgttcg ttctttgtcg
    22141 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    22201 ctcctgcgcc tctccacacg gagaggcgtt tttatcgctg cgcaatgttg actcttgtca
    22261 caggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    22321 taaataacaa aataccgatg gcgattttcg tccaccatga gctcagcgtg ccatcaaagt
    22381 tgatataagt ctgtattagc ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    22441 caacgccacc gctcagaagc gtaccgccaa ttaccactga ggcgatagca tccagttcca
    22501 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    22561 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    22621 ccatcaggtt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    22681 tacgatgcgc gaggaaaata ccgataacca ccaccgccaa catcagcagt cccatcgcac
    22741 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    22801 ggtttatcgg aatcgactct tccgaaacga gatagctgac accgcgcaaa aagaacatcc
    22861 ccgccagggt aatgataaat gccgggatct tcagggcgtc gatcaaaagc cccataaatg
    22921 cgccgaaggc acagcccatc accagcacta gcggaaacgc cagtagcggc gagaggccga
    22981 aatcgccaat cacttttgcc agaaacacgc cagtaaaggc gatcaccgaa ccgacggaga
    23041 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    23101 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccggga aactgggtca
    23161 ggcagtaaag ataacccaac acaaagacgc cgatggtgat catcagtggc aaattacgtt
    23221 ttatcacggc tacgtactcc tttaatcaga ctgataaagc gttgcgactg gacaatcagc
    23281 acgcaaagaa ccaccaccgc ttttacaacc tggttcatct ctggcggaaa gcccgaaagc
    23341 aaaattccgg tgttcatccc ctgaataatc agcgccccca ccaccgaaag cagtaggtta
    23401 aaacgtccgc ccatcagcga tccgccgcca ataaccaccg cgagaatggc gtccagctcc
    23461 agccataacc cggcgttatt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    23521 gcaatcgccg cacacagccc gctcaacacg taggtaagca tgacgatgat ccgcgtgttt
    23581 accccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    23641 gccgttttgc gggtcaacag ccagaacagg ataagcgtca gcaccgcgat aatgaccggc
    23701 gttggcagga acaacagcga tccactgcca aaccatgaga gatccggcga gttaaacgtg
    23761 acgatctgtc cggaggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    23821 gtggcaacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgcc
    23881 aggatgccag tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    23941 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacggagag atcaatccca
    24001 cctgttgcga tcaccagcgt cattccgata gccaacagcg caacgggggc cgcacggtta
    24061 agaatgtcta tggggctacc gaataaacgc ccatcctgga gcaccacctg ccagaaatgc
    24121 ggggccacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcattccc
    24181 gttggccagc gaaagcgcct tttcggcggc gtagtgtccg ggagagattg aggcatcaca
    24241 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct ctgccagcgg
    24301 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    24361 ttcttccagt tcggaggaga tcaccagcag cgccagacca tcggcgcata gcgtttcaat
    24421 caggcggatg atctcggcgt gggcaccaac atcaatgccg cgagttggct catcaaggat
    24481 cagaaattgc ggtcgggtca gtagccaacg tgaaagcaac actttttgct gattgccgcc
    24541 agagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    24601 gcgttcggca atctcttgct gttctttgcg ggaaatggga cgtagccagc cgcgctgggc
    24661 ctggagagcg aggatgatat tttcccgcac cgaggcggca gcgatgatgc catcggtttt
    24721 cctgtcttcc gggcagaagc caatgcccag taccgaagcc tgatgtggcg atcgcaggtt
    24781 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    24841 ttcggcggtt tcggtacgtc ctgatcccag caatccagcc agaccgacga tctcgccggg
    24901 gcgtacttcg agatcaaacg gtgcgatcgt tccttttttg ccgtaatttt tgaacgcggc
    24961 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcgcgtggg tatccagctc
    25021 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcacgttt cccgacagcc
    25081 tacgaaactg ccgttgcgta agacggtgat ccgatcgctg acctgataga cctgatcgag
    25141 aaagtgagtg acaaaaatca ggctgacgcc gcgatcgcgc aactgacgca tcaggtcaaa
    25201 cagtaactcc acttcctggg tgtcgagact ggcggtgggt tcatcgagga tcagcacttt
    25261 ggcagagaga tcgatagccc ggcaaatagc gacgatttgc tgcatcgcga ctgaaaagcg
    25321 gttgagcggt tcgcgcacgt cgagggagaa accgtaagat gccatcagtt cggtggcgcg
    25381 cttttccatc tctttgcggc gtagaaggcc gaagcgtttg ggttcgcggc ctataaatag
    25441 attatcagcg accgacatat tgggtagcag gtttacttcc tgatagacgg tgccgatacc
    25501 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    25561 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcctgcccc
    25621 gttttcaccg agcagcgcca tgatttcgcc gcggcgcagg ctgaaatcaa cgttgtctaa
    25681 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    25741 gttagtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    25801 gtaagcgtcg catcaggctt cagcgcacca ccgccggatg cggcgcgagc gccttatccg
    25861 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    25921 actcttcttt tgcggtatca ggcagataaa gggtggattt ggtcagcgtc agcttttcag
    25981 gcatggtgcc gtcttttttg tatttctcca gcgcgtcgaa ggcgggacct gccatattcg
    26041 gcgtcagttc aacactggcg ttcgcttcgc catcaatcat cgctttgtag atatccggta
    26101 cgccgtcgat agaacctgtc agaatatctt tgcctggttt caggcccgct tctttaattg
    26161 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    26221 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    26281 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    26341 ctttcttacg gtcaatggcg acgctggccc caacagtgcc ctgcagctcc accacgttgc
    26401 atggtttgcc attcacttct tttaccagcc agtcaccaat caacttgcct tcgagaatgt
    26461 tgtcggcagt gacggtggtc atatagagag atttgtcttt cacatcaata gaacgatcga
    26521 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caaccagtcg
    26581 caaccaccgg agcgataaag atcgcatcca ccccttgcgc gacgaaggaa cgtaccgctt
    26641 taatctggtt ttcctgcttt tgctgaccat cggcaatttt cagcgtaatt ccgcgttttt
    26701 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccagattcc gatccgacct
    26761 gcgaaaatcc tacggttaat ggagcggcca acgccataga cgacatggct gccgagactg
    26821 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    26881 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    26941 acacttcaaa gaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    27001 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaattggcga
    27061 aaaaggaaaa tgaagacgaa aacaagcgaa gacattcggc gcgagttggc tataatactc
    27121 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    27181 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    27241 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    27301 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    27361 cccggttgac gtactggtcc cgactccgta cccgctgcag ccgggttctg tgatccgttg
    27421 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactgattgc
    27481 tgttccgcac acaaagctga gcaaagaata cgatcacatt aaagacgtta acgatctgcc
    27541 agagctgctg aaagcgcaga tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    27601 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    27661 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    27721 cttcggttag taagggtttt tttatgcccg cgataaataa actctctatt ccaccatcat
    27781 tattctcagc ggttgcaagg cttgaacggt aagaacaagc aaacccgacc accattttgc
    27841 tgttcatagc cacttgctgg aagttagccg acctcactca tactcaccgt tcaaagcgtg
    27901 cccccgtttt acccctgcac atttgtgaac gccagcagag ttttttatgc gattgcagat
    27961 atggaaagcg gctcggagag aaaagcaaaa gatggaaaag ttacaaaaaa aaatcgcgag
    28021 atacatcgca taaaacaacg ccacccgaag gtggcgtgct tttattatta cttatccctg
    28081 tctaaccagt cgccgctttc aattagcttt aatccatcga cgggtcgttg ataaacgtac
    28141 atccatgcac tcccgtacgg cgtctgaatc aactggcgcg cgtattcacc gcccctggtg
    28201 cgcaaggcat caagttcggc cagcgtggcg ttgtcaatac gataaacttc accgtgtact
    28261 gttccgttcc ccggaactgc gcctggatag tggcccaggc tatacaactg gtagttatcg
    28321 atactgaaat cgcccagtaa ctgggcattg gtcatccagt gactgttgcc ttgtttgtgg
    28381 cgtaaactgc cgtagacaaa tattcgcatt gctaaaactc gaactgatag agcaaatcca
    28441 gtgcctggtc tacaccagac acggcttcca gatatagctt aggcatcagg cgataacgta
    28501 acgtgagtgt tgctatagag tcaaatatac ccacgccgta tttcacttgc agacctggca
    28561 atacatagcc gctgaccact acctgggagg agtcgcctac tccctgggtg tcgagcgcta
    28621 aattgcttac gccaaacgtc tcgccgattt tacccacaat ctggccactt tgcgcaaccc
    28681 ccagaccaat cagcatcgag gtcattgccg cactgtcgct ctgatcgctc tccagccctt
    28741 gtccacgtag caaataagac aatgcagctt gttgcgacat cgccgggtca gagaagatct
    28801 ccgctttcgg ttcgtccgcc agaccagtga cgcgaactcc ggcgattacg tcgtcttctg
    28861 tagcatccgg gttacgaata gcttcaatat taagatacgg ttgatccggc ggaccagaga
    28921 acagtaactc acctttacgc acaatcagat cctgaccata ggcatggaag cgcccttcag
    28981 ggatgttgat ctgcccgttc aggcccagcc cttgtttgtc ctgaatgacg ttaagatcgc
    29041 ccgtcagccg cgctttcagg ccaaaggcgt caatgcgcac attgttgcca acgtggacaa
    29101 tcaggttact gttaatcgga atcgacgccg ttttcggctc ttccggttgc aggttatcgt
    29161 taagcatcac cacatcgctg gagacgccta ctgcgctttc cggcagatcg tgcaccacga
    29221 tgcgcgccca cgggacatcc acgcgaccat cgagggtaaa caggtttggt gtagcctcga
    29281 agacaacatc tggcgataca tccattcgta ccatcggcgg cacggtgatc cgcactttac
    29341 tgcctttcgc cgttacccgc gcccgccagt tttcaatttg gctccagtcg gcatcaccgt
    29401 tcaggtagat ttcaccctgc tgggtccgta ctgtaccggc aagcgtcgag cgcataccat
    29461 taaagttgac cgcaagctgg ctcggttgca tatcaaacgg cataaagttg ccgtcgatat
    29521 ccacacccgt aacctgaagc tgaccaaaca actgcgggct ttgcacatca ccacccagac
    29581 gcaagttggc actcaccatc cccgctgctt tttccccacg ggtaaagatg gggtttatca
    29641 tcgcaaggtt gaagttacgg atattgacgt tgccaccaag attacggcgg ccttgcggat
    29701 cggtcacctg cacctgtcca tcaaactggc cgttattggt caggcggatg gtccagccca
    29761 attcggcacg gttgttacgc aattccgccg tcagattcag tgtctgaaac gccacgggca
    29821 gcgccgcatc gttgacggtt tgcgttacct gcacgttacg ccccgaaagg gtgatactgc
    29881 cctgcggcag cccctctttg gtggtgtccc aggcaacgtc cgctttaccc gtgaagatac
    29941 cgctggcctg agtggtttct ggcataaatg gtttcagcat ggccaggtcg aagcggttga
    30001 gattcaccac cgcacgccct tcggcacccg catcgatagt ttgcggcacg cacagttccg
    30061 cattcgggtt aagccagcag tgtggcccga tgctgatttt ttgctcctta ttgcggtaat
    30121 ccagcgcaat atcgcgggtc agcgaccacg ggccgaccgg cgtctggaag cgggtattgc
    30181 taagagttcc cttccagcgt tcttctttgc gatcaaaact tcctgccaga ttaagctgcc
    30241 cggagacagg ctcgccctga atccgcaact gtaactcgtg ctgtttttcg ctgcctttgg
    30301 cattcagggt gacgaggttg atatttacat ccggctgcga aatttgctca acgcgtacgt
    30361 cgagtttccc ggcgatctga tcggtggatt taatgtcgcc ttccacgcga acctgcgcca
    30421 cggaaagttc ctgccagcgc aggccgcgcg cggtgatatc tgccagtagt tgtggcgctt
    30481 ccaccgtgcc gcgtactttc accagccctt tcgctgtacc gccaagcccc ggcagcgcgt
    30541 tatccagccc cggcgcgttg atggtggcat caagattgag atcttttacc cccagctcgc
    30601 ctttcacttc ggcactgttt ggccccagtt ccagatgaag ccctgggatc atccactgca
    30661 tataactgtt gcctttcagc gtgccgtcaa cgttcacttt gttctgtttg acgttaccgg
    30721 tcagcttcag ttctggcacc tccatctgcc aggtgccgcc gtacaggcta ccgcgggttt
    30781 taatcaagcc attgagtttc gacggccact ctgggatctc tttggcggtg ttaatgccgt
    30841 taagcgttag ctcaccgcgc caactaatgg cctgctgcca gtcgagcaac gccttgagtt
    30901 cagttttccc ttccagcgcc gcgacggtga gtttgtcgag attgacctgc tgttcattac
    30961 ctttggcatc aagggtaatg gtggctggcg ggatctccag tcccttcact gccgtacgca
    31021 tagagagcgt gtaatcggtc attttgccgg taagtttcag tttcagatca tccgcctgat
    31081 actgcttctc accagtgaac ggccagtaaa tctgtttgct gttcacttcc acgttgagcg
    31141 gcaatccggc ttccgccagt cgcgtctggg cgcgtaaatc catatccacc ggaccggaaa
    31201 gattaacgcc aatctccagc tgttcgcgca gcgcgccgcc cactttcagc ttcacctttt
    31261 cacctttcaa cggctccacg ttcagtgtac tattgagagt gatgtccacc ggccagttgt
    31321 ctgacagctg cgccgtgccg ctggcgttga cgatcccttg actggaatcg atatccaggg
    31381 cgtccagttt agtattgccg tcaatgctgc tcactttcag cagcatggtg cgcacggtga
    31441 tgtccgtgtc gcccgtcacg cgtaactgct cgcctttaaa ctcttcaatg ttcaggttaa
    31501 gcggcaaatg cacgtcggtc atttccggca ataccgggcg agaaaagaga tctttcagcg
    31561 tttcgccgag cggcttttcc tccggctgcg gattttcaat tttcggttcg accacttctt
    31621 cctgcgccac ttccgccact ttcggcagag caatcagcag gcctttcagc gacgtcggtt
    31681 tcagggtcag ggttttctcc tgccagttca ggccggaggt gaagtccatc accgataccg
    31741 tggtgtcatc aatcttgatg ttgacgttgt ccagtgccac ccgtgttagg gtgatgggat
    31801 acggcgtgga gagatccagc ggaccgctat cttcctcttc ttcaacctgt tcagaagggg
    31861 gcattttttt gctgtcgata ttgacctgga tgtctttcag cgccagatca ttaatacaaa
    31921 cactgctgtt ccacaggcac tcaagcccga ccgccagatg cagattgcct gcttttaccg
    31981 ccacgcctgg ctgctcataa cgaacgtcag acaaggtgag atcgcgccag ccgccggtga
    32041 ccttgccaac atccagtcct ggcacccagc gatccgccgc tttaaatacc agatgcaggc
    32101 cgctggtggt gcccaccaga aacgccaccg atcccagcaa cagtaagata acgataacca
    32161 cgccgaggct gatttttttc cataaactca taattctggc cccagaccga tgtaaaactg
    32221 taacccgtgt tcgtctttat ccgcgaccgg tacggcaaaa tcgagtttga ttggcccgac
    32281 cggcgattcc cagcgtacgc cgaccccggt accggtttta aagtcgctgc ggcgaatatc
    32341 gcttaccgct tcgccactat cgacaaacac cgcgccccac cattttccgg tcacgttgta
    32401 ctggtactcc agcgatccgg ttatcaactt cgaggcccct ttcaggtcac cgttggcgta
    32461 tttcggagcg atagatttgt atttgtagcc acgaatactg cggtcgcccc cggcgaaaaa
    32521 acgcagatcc ggcggtactt tgtcgaaatc accggtttca atccagccca gcgtgccgcg
    32581 tgtcacaaaa cgatggcgat cgtacagtgt gcggatccag acgttttgtg cctggaaaac
    32641 ggagaaatcg acatctgagc cccaggcagt gttggagtag tcgatagagt agcgttgcga
    32701 gtcgccccag gttggcatca ggccaccacg agaacgcgtg cggctaatca tcaccccagg
    32761 ataaaacagc atcgtggtgt tggtaatttc accctgggta aagtggtcga gactccagcg
    32821 caggttaatg gcacgctgcc agccgctgga gagatcccag tagcgagaag ccaccagcgt
    32881 agtggagtca gattcggtat cgttcaggtc agtgcgctta aaaccgccct gcaccaaata
    32941 atattgttcc agtggattct tcagcagcgg cattttatag ctgaagtcga ggatctgttc
    33001 cggcgcggaa atactggtac tggtggtcag actgtgaccg tatgagttca tccacggttt
    33061 tttccacgtc gctttcacgc gcggtcccac gtccgtagag taaccgaccc cggtttcgat
    33121 ggtgttttct gttcgcggcg aaaccacgcc cgtcaatggt aatactttcg tttcgcgcgc
    33181 tttatcaaat tgtggagcca ccaccaccga gttaaaccag ccggtagcag aaagtcggcg
    33241 gttcagttcc gccagatctt tcgattcgta ctcatcgccc tctttaaacg gcaccagatt
    33301 ttgcaggtat tcatcgcgga tttgtgatcc ttcaaaggtc acatgcccaa agcggtaacg
    33361 ttcgccactg ttataatcaa tatcccagaa ggctttatgc aggccgagcg caatgcccag
    33421 ctgcgctttg gtaaattcgc tatcgaaata acctttacgc aacgcaatgc tggttaagga
    33481 ctttttgaaa ttttcataat cgccctggtt cagcaccgtg ccaatagccg ggcgagtatc
    33541 gagcaatttc aaatagtctt tatcggtccg cgcgccgccg cgcaatacca catcggtgcc
    33601 gccaattaac accggcacgc ctggcgtgac tttggcgatc aatacctgcc gccctttctt
    33661 tggcggtgga cggagatcaa attcaatggt cggctggtaa taacccagcg ctttcagacc
    33721 ttcgcggatg gcatcatcga cgcgtgcgcg aaagcgacgg tctggcgtca cttcatcact
    33781 ttcaatcgta gaaagctgcg cacgaacgtt cttttccagc tgtcccgata acccctcgac
    33841 ctgtagacgg acgttcgcgg cgacggcaga tccgcttaag cagagtaagc ttacacagca
    33901 taactgtcgg atatagcgca cattttctcc tgaatatcct tttttcctgc ccctggaacg
    33961 ccgttaaacg gcttaacaaa aatccagtaa tatggattaa aaagcagact aaaccccaaa
    34021 tatttcttat gttttacttt agacctattc acggtggtta ttgtgtgcaa atacgcctct
    34081 tgttacaacc ttaaccccaa tgaccgattt tcgggagagc gacaccatga gtttatttga
    34141 taaaaagcat ctggtttccc ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt
    34201 agccacgctg catgcggtca acggtcactc aatgaccaat gtacctgacg gaatggagat
    34261 tgccattttt gcgatgggtt gtttctgggg tgtggagcgt ctgttctggc agttacccgg
    34321 cgtttacagc accgccgcag gctataccgg aggctatacg ccaaatccga cttatcggga
    34381 agtgtgctcc ggtgatacgg gtcatgccga agcggtacgc attgtttacg atccttccgt
    34441 catcagctat gagcagttgc tacaggtatt ttgggagaat cacgatcccg cccagggaat
    34501 gcgtcagggc aatgaccacg gcacgcagta tcgttcagcg atttatccgc tgaccccaga
    34561 acaggatgcc gcagctcgcg ccagtctgga acgttttcag gcggcgatgc ttgccgccga
    34621 tgatgatcgt cacatcacca cggaaatcgc taacgccaca ccgttttatt atgccgaaga
    34681 tgaccaccag caatatctgc ataaaaaccc gtatggttac tgtggaattg gcggaattgg
    34741 cgtctgtctg ccaccggaag catagcgtta cgggtacaaa tgtagattgt tgataaagtg
    34801 cgctttattt atgccagatg cggcgtaaac gccttaccag gcctacaaaa tcgtccaaat
    34861 tcaatatatt gcaaggactg cgtaggcctg attggcatag cgcatcaggc agtattgcat
    34921 ttatcagcag tctgaatttt aaccctctgg cgactttaca gtaccttacg ctatactagc
    34981 cactgaaaat gccggttcac tttcttcgaa tcggctttca atgtgtattt cacacaaatt
    35041 aatcaacttc cccttccgag gatctggcct gaaaggtcgg ataagatatg ttaaacagta
    35101 ttttagtcat actctgcttg atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcac
    35161 ttgccgcctc acgcaaaatc aaacttaaac tgctggctga tgaaggcaat ataaatgccc
    35221 aacgcgttct gaatatgcag gaaaatcccg gcatgttctt taccgtggtc caaatcggtc
    35281 tgaacgcagt ggcgattctc ggcggtatcg tcggtgatgc ggcattttct ccagcttttc
    35341 acagcctgtt ctcccgctat atgtcggcag agctctctga gcaactgagc tttattctct
    35401 ctttctcgtt agtgactggc atgtttatcc tgtttgcgga tttaaccccg aaacgcatcg
    35461 gtatgattgc gccagaagct gtcgctttgc gtatcatcaa cccgatgcgc ttctgcctgt
    35521 acgtttgcac cccgctggtg tggttcttca acggactggc gaacataatc ttccgtattt
    35581 tcaaactgcc aatggtacgt aaagatgaca tcacttctga tgacatctac gcggtagtgg
    35641 aagccggtgc gctggcgggc gtgttacgta aacaggaaca cgagctgatt gaaaacgtct
    35701 ttgagctgga atcccgtacc gttccgtcgt caatgacacc gcgtgaaaac gtgatttggt
    35761 ttgatctcca cgaagatgag caaagcctga agaataaggt ggcggaacat ccgcactcta
    35821 agttcctcgt ctgtaatgaa gatattgacc acatcatcgg ctatgtcgat tctaaagacc
    35881 tgctgaaccg cgtgctggct aaccaaagcc tggcactgaa cagcggcgta caaattcgca
    35941 acacgctgat tgtgccggat acgttaaccc tttcagaggc gttggaaagt tttaaaaccg
    36001 caggtgaaga cttcgcggtg atcatgaacg agtacgcgct ggtggtgggg atcatcaccc
    36061 tcaatgacgt gatgaccacg ctgatgggcg atctagtcgg tcaggggctg gaagagcaga
    36121 ttgtcgcccg tgatgagaac tcatggctga ttgacggcgg caccccaatt gacgacgtca
    36181 tgcgcgtgct ggatattgac gagttcccgc agtcgggcaa ctacgaaacc atcggcggct
    36241 ttatgatgtt tatgctgcgt aagatcccga aacgcaccga ttcggtgaaa ttcgccggct
    36301 acaaatttga agtggtggat atcgataact accgcatcga ccagctgctg gtgacccgga
    36361 tcgacagcaa ggccaccgcc ctttcgccaa aactgcctga cgctaaagat aaagaagaaa
    36421 gcgtcgcgta acccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    36481 atagcacttt ggttaagcca tctctgtttg cagacgcata acctgacggt taacttcgga
    36541 catcactgac aaatgcagct tatccttcac ttttgggata agaatcttac ccttatcaaa
    36601 ttcaaaagcg ccaacgtcct tgatgtataa ccgtccacgg aacaggatct ttacgtactt
    36661 cgccacctga agtgggttgt accgttggaa aattttcatt gttatctcct gctgagtatt
    36721 acgcctgtgc ggtgccacaa tcggcccaac tattatgagg cgcaaatttt aatgcttagt
    36781 gactatagac tatccgggca atgtttccac cgcgtataac tttttttacc taaaggttac
    36841 aattattcag aattatcttt ttaccgaagc gcggtcttca gtataagcat tcatttttca
    36901 tatgatttgt gcgcttgacc gcaaactggc atcacacttg cgggaaattc gataaatagc
    36961 acatatgatt aaaactcaga cccaagtggt cggatcacct gcatatcata agaaggaaac
    37021 accatgaccc tacgcaagat tctggcactc acctgcctgc tgttgccgat gatggcttcc
    37081 gcacatcagt tcgaaaccgg tcagcgagtg ccgccgattg gcattaccga tcgaggcgag
    37141 ttggtgcttg ataaagatca gtttagctac aaaacctgga acagcgcaca gttagtggga
    37201 aaagtgcgag tactgcaaca tattgctggt cgcacctctg caaaagagaa aaacgcgacg
    37261 ctgattgaag cgattaaatc agcgaagtta ccgcacgatc gctaccagac caccaccatt
    37321 gttaacaccg acgacgcgat tccgggttca gggatgtttg tgcgcagcag tctggagagt
    37381 aataaaaagc tttatccctg gtcgcagttt attgtcgata gcaatggcgt cgcgcgcggt
    37441 gcctggcagc tggatgaaga aagttccgct gtagtggtgc tggataagga tggtcgcgtg
    37501 caatgggcca aagacggggc gctcacacag gaagaggtgc agcaagtgat ggacctgctg
    37561 cataaattaa ttaataaata gacactctga atcccggatt caggaacgac tcgcgcggag
    37621 tgtaatccag cggtttaccc tgccagtcat gaacgtgcgc tccggcagct gcagccacag
    37681 catgaccagc ggcggtatcc caaatattcg ttggtccgaa gcgcgggtac agctgcgcct
    37741 gcccttccgc caccaggcag aatttcagcg aagagccgat ggacgtggtc tgatgttcgc
    37801 caagctgttg cagatactct ttcagctccg catctgcatg ggaacggctg ataaccacca
    37861 gcggcgggcg cgcatcgcgg acctgaatct gcttgcgcac accgcactct tctttccagg
    37921 ccttgccttc tgccgcgctg tacattatgt tcattaccgg cgcatacacc acacctaaaa
    37981 tcggtttgcc atggtcaatg agcgcaatgt taacggtgaa ttcgccatta cgtttaataa
    38041 actctttagt accatccagc gggtctacca gccagtaacg ctgccagtgc tgacggactt
    38101 cccaaccggg aggatcttct tcagaaagga ccggaatatc cggtgtcagc gtacgtaaac
    38161 cgtccatgat aacggtgtga gcggcaatat ccgctgccgt taccggagaa ttgtccgctt
    38221 tgctgacgac gtccatcggt ttcgtcccgt cgtagacctg cataatggca tcgcctgcat
    38281 tccgtgcaag ctggcatact tgatctaaca tttctccacc tcgtctctgt gagcggtgtt
    38341 aacttattgt tttacttata ccctatcgtt aatgaatgcg ccaactgtga tagtgtcatc
    38401 attttcaaag cgtaaaattg tggcattctt cactgttcta taagtaaggc gtttattctc
    38461 ccttttcttt cgtattcccg atgataaaag gatgtccctg atgattaagt ttagcgcaac
    38521 gctcctggcc acgctgattg ccgccagtgt gaatgcagcg acggtcgatc tacgtatcat
    38581 ggaaaccact gatctgcata gcaacatgat ggatttcgat tattacaaag acaccgccac
    38641 ggaaaaattc ggactggtac gtacggcaag cctgattaac gatgcccgca atgaagtgaa
    38701 aaacagcgta ctggtcgata acggcgattt gattcagggg agtccgctgg ccgattacat
    38761 atcggcgaaa ggattaaaag caggtgatgt tcatccggtt tataaggcgc tgaatacgct
    38821 ggattatacg gtcggtacac tcggcaatca tgaatttaac tacggtctgg attacctgaa
    38881 aaatgcgttg gcgggagcga aattccctta tgtaaatgcc aacgtcattg acgccagaac
    38941 caaacagcca atgtttacac cgtatttaat taaagatacc gaagtggtcg ataaagacgg
    39001 aaaaaaacag acgctgaaga ttggctatat tggcgtcgtg ccgccgcaaa tcatgggctg
    39061 ggataaagct aatttatccg gaaaagtgac ggtgaatgat attaccgaaa ccgtgcgcaa
    39121 atacgtgcct gaaatgcgcg agaaaggtgc cgatgtcgtt gtcgttctgg cgcattccgg
    39181 gctatctgcc gatccgtata aagtgatggc ggaaaactca gtttattacc tcagtgaaat
    39241 tcctggcgtt aacgccatta tgtttggcca tgctcacgcc gttttcccag gtaaagattt
    39301 tgctgatatc gaaggggctg atatcgcgaa aggcacgctg aatggtgttc cggcggtaat
    39361 gccgggcatg tggggcgatc atcttggggt ggtcgactta caactcagta ataacagcgg
    39421 taaatggcag gtgacgcagg cgaaagcgga agcacggccg atttacgaca tcgccaataa
    39481 aaaatccctc gcggcggaag acagcaagct ggtagaaaca ctcaaagccg atcacgatgc
    39541 cacacgccag ttcgtcagca agccaatcgg taaatccgcc gacaatatgt atagctatct
    39601 ggcgctggtg caggacgatc cgaccgtgca ggtggtgaac aacgcgcaaa aagcgtatgt
    39661 cgagcattac attcagggcg atccggatct ggcaaaactg ccggtgcttt cagctgctgc
    39721 accgtttaaa gttggtggtc gcaaaaatga tccggcaagc tatgtggagg tggaaaaagg
    39781 ccagttgacc ttccgtaatg ccgccgatct ttatctctac cccaatacgc tgattgtggt
    39841 gaaagccagc ggtaaagagg tgaaagagtg gctggagtgc tccgccggac agtttaacca
    39901 gattgatcct gacaacacga aaccacagtc actcatcaac tgggatggtt tccgcaccta
    39961 taactttgat gtgattgatg gtgtgaatta tcagattgat gttacccagc ctgcccgtta
    40021 tgacggcgag tgccagatgg ttaatgccaa tgcggaaagg attaagaacc tgacctttaa
    40081 tggcaagccg attgatccga acgccatgtt cctggttgcc accaataact atcgcgctta
    40141 cggcggcaaa tttgccggta cgggcgacag ccatatcgct tttgcttcac cggatgagaa
    40201 ccgctcggtg ctggcagcgt ggattgctga tgagtcgaaa cgtgcggggg aaattcaccc
    40261 ggcggcagat aacaactggc gtttagcacc gatagctggc gataagaaac tggatatccg
    40321 tttcgaaacc tctccatcag ataaagccgc ggcgtttatt aaagagaaag ggcaatatcc
    40381 gatgaataaa gtcgcgaccg atgatatcgg gtttgcgatt tatcaggtgg atttgagtaa
    40441 gtaatacact tctttttcgg cctataaatc atcaaccgca tccggcactt ttgtcggatg
    40501 cgatgctggc gcatcttatc cgccctacaa gtcatgaacc gtaggccgga taagcgcagc
    40561 cggcagttat gccgcacgtt catcccgcac cgccaacacc tcgggcaaat tcaactcaat
    40621 ccagtccgcc agtgcagcaa ccttttcgct cacctgctcg cccagcggcg tgaggctata
    40681 ttccacatgc ggcggcacca ccggatacgc gatacggtta agaaaaccat cctgttctaa
    40741 cgcctgtaac gactgcgcaa gcatcttttc actcacccca ccgattttgc gccgcaggtc
    40801 gctaaagcga tgagtacctt cgcgtagcgc caccagaatc aacacccccc aacggctggt
    40861 gacgtgtttc aacacctcgc gcgacgggca ctgttccgca aagagattac cctctttcag
    40921 ttgttgtgac aggctaacct gactcatttc atacttacct ttttgtacgt acttactaaa
    40981 agtaagttta ggtgttagcg tatttaaaca caagacaaaa cgatggagac tccccatgat
    41041 cgctattact ggtgccactg gccaacttgg tcactatgtt attaaatcct tgatgaaaac
    41101 ggttcctgcc agccaaatag tggctatcgt tcgtaatccg gcaaaagccc aggcactgac
    41161 agcacaaggc attaccgtgc gtcaggctga ctacggcgat gaagccgcac tgacatctgc
    41221 acttcaggga gtggaaaaac tactgctgat ctcttccagc gaagtgggtc aacgtgcccc
    41281 gcagcatcgt aatgttatta atgccgcaaa gacggctggc gtgaaattta tcgcttatac
    41341 cagtctgctt cacgcggata aatctccgct cggcctcgcc gatgagcaca tcgagacgga
    41401 gaaaatgttg gctgattctg gcatcgttta caccctgctg cgcaacggct ggtacaccga
    41461 aaactatctc gccagcgccc cggcagcact ggagcacggc gtatttatcg gtgccgcggg
    41521 cgatggcaaa attgcctcgg caacgcgggc agattatgcg gcagctgcgg cacgcgtgat
    41581 tagcgaagcc ggtcacgaag gcaaggttta cgagctggcg ggcgatagtg cctggacgtt
    41641 gacacagtta gcggcagagc tgaccaaaca gagcggcaaa caggttacct atcaaaatct
    41701 gagcgaaacc gatttcgctg cggcgctgaa aagcgttggc ctgcctgacg gactggcgga
    41761 tatgctggcg gattctgacg ttggcgcatc gaaaggcggt ctgtttgatg acagcaaaac
    41821 gctgagcaaa ttgattggcc gcccaacgac aacgttagcc gaaagcgtaa gccatctttt
    41881 taatgttaat aaatagttaa ttaaagtggc atcctcccgc atcctctctg ataatgacgg
    41941 gatgccggga gcaatcatgt ctgcttcctg aacgtttctt ctgacagacc aatggatgcc
    42001 agtaatgatt agcggcgtgc tgtacgccct gttagcaggg ttgatgtggg ggcttatttt
    42061 tgtcgggccg ttgatcgtgc cggaataccc ggcgatgttg cagtcgatgg ggcgttatct
    42121 ggcgttaggg ttaattgcgc tacccattgc ctggctggga cgcgtgcgtc tgcgtcagtt
    42181 ggcgcgtcgc gactggctta ccgccttgat gctcactatg atgggcaacc tcatctatta
    42241 cttctgcctt gccagtgcca ttcaacgtac tggcgcgcct gtttctacga tgattattgg
    42301 caccctgccg gtggtcattc ctgtctttgc caatctgctt tatagccagc gcgacggcaa
    42361 actcgcgtgg ggaaaactcg ccccggcact gatttgtatt ggcatcggcc tggcgtgtgt
    42421 gaatattgct gagttaaacc acggactccc cgattttgac tgggcacgtt atacctctgg
    42481 catcgtgcta gcgttagttt ccgtggtctg ctgggcatgg tatgccctgc gcaacgcccg
    42541 ctggctgcgg gaaaatcccg acaaacatcc gatgatgtgg gcgacggcgc aggcgctggt
    42601 cacgctgccg gtttctctca tcggctatct cgtcgcctgt tactggctga atatacaaac
    42661 gccggacttc tccttacctt ttggcccccg tccgctggtg tttattagtc tgatggttgc
    42721 gatagccgtg ctttgctcat gggttggcgc actctgctgg aacgtcgcca gccagcgatt
    42781 accgacagtg attctcgggc cgctgatcgt tttcgaaaca ctggcaggtt tgctgtacac
    42841 ctttttgata cgccagcaaa tgccgccgct gatgacgctg agcggtatcg cgctgttagt
    42901 ggttggcgtg gtcattgcag tcagagcaaa accggaaaag cctttaactg aatctgtctc
    42961 agaaagttga catactggca gtgagttaaa taagcctctg ctacgtaagg gttatagcat
    43021 ttgccttaaa gatgcattta aaatacatct tatcttatta agaatgaggt atcagctatg
    43081 gcttatcgcg accaaccttt aggtgaactg gcgctctcta tccctcgcgc ttcggctctg
    43141 tttcgtaaat atgatatgga ttactgctgc ggcggtaagc aaacgctggc gcgtgcggcg
    43201 gcacgtaaag aactggatgt tgaggtcatt gaagctgagc tggcaaagct tgctgaacaa
    43261 ccgattgaga aagactggcg tagcgccccg ctggcagaaa tcatcgacca tatcatcgtg
    43321 cgctaccacg atcgtcaccg cgagcaactg ccggagctga tcctgcaagc gactaaagtc
    43381 gagcgcgttc acgccgacaa accgagcgtg ccaaaagggc tgacaaaata cctgaccatg
    43441 ctgcatgaag agctttccag ccacatgatg aaagaagagc agatcctctt cccgatgatc
    43501 aaacaaggca tgggcagcca ggcaatgggg ccaatcagcg taatggaaag cgagcacgat
    43561 gaagcgggcg aactgctgga agtgatcaaa cacactacca ataacgtcac accgccgcca
    43621 gaagcctgca ccacctggaa agcgatgtat aacggcatta atgaactgat tgatgacctg
    43681 atggatcaca tcagtctgga aaacaatgta ctgttcccac gcgcgctggc gggtgagtaa
    43741 tctaaaagct ggatggcatt gcgccatcca gcatgataat gcggttattt ccgcagttca
    43801 gcagcccgct tcttaccaat aaacaaccag cccagcccca gtgcgataaa ccacagcggg
    43861 gtgatcaaca gcgcctggcg agtgtcatct tccagtgtca gcaacacgac cacgaacaca
    43921 aagaacgcca tacataccca gcacatcagc ttgccgagcg gcatcttgta gatcgacttc
    43981 tcatgcagat gaggacgctg tttgcggtaa accaggtacg agcaaaggat aatcgtccag
    44041 acgaacataa acagaatcgc ggaaacggtt gtaatcatcg tgaacgcgcc aatcacgcta
    44101 ggattcacat acagcatcac cacgccaccg agcaggcaga tgcaggagaa ggtcagccct
    44161 ttcgctggta ctgcgcgctt agaaagttta gcgaacgctt tcggtgccac accttcctgc
    44221 gccagaccaa acagcatacg gctggtagag aagacgccgc tgttagcgga agacgccgca
    44281 gaggtcagca ccacaaagtt gatcacgctt gcggcagcag gcagccctac caacacgaac
    44341 agttcaacaa acgggctttt ctccgggact accgaactcc acggcgtcac ggacataatc
    44401 acaatcagcg cgaagacgta gaacataatg atacggatcg gaatggagtt aatcgcgcgt
    44461 ggcagtgatt tctccggatc tttggtttcc gcagctgttg tacctaccag ctcaatcccc
    44521 acgaaagcga aaaccgctat ctggaatccg gcaaagaagc cacttaaacc tttcgggaac
    44581 cagccgccgt cattccacaa atgcacgaat gacgcttcca caccggtcgg tgactgaaag
    44641 tgcatcgcca ccatgaccag gccgacgaca atcagcgaca cgatggcgac gattttgatc
    44701 atcgcaaacc agaactccat ctcaccgaac attttcacgg tggcaaggtt gagcgtcaac
    44761 agcagcacta tcaccgccag cgaggcgacc cagtcggaga gatcggggaa ccagaactga
    44821 gcataagcag tgatcgccac cacgtctgcc ataccggtta caacccagca gaaccagtaa
    44881 gtccagccgg tgaaatatcc tgcccacggc ccgagtaaat cggaagcgaa gtcactaaaa
    44941 gatttgtatt ccagattcga aagcagcaat tcccccattg cccgcatcac gaaaaagagc
    45001 ataaaaccga tgatcatata aacgaaaatg atcgacggcc cggcaaggct aatcgttttg
    45061 ccagacccca taaacaaccc cgtaccaatg gcaccgccaa tggcaataag ctgaatatgt
    45121 cggtttgtga gattgcgccg tagcgactgt tcagccggag cctgatcatc ggcaacgact
    45181 tttacctgat ctaccatgtt tttttcttcc tgtacctgtc tgtgttgttc aggctctacg
    45241 gcctttagtc agtctatgat acgacgaatc tgtatcggga ctcatcgata ttaggtaaca
    45301 atacgcggga tgaatactaa gatttagatc taatgttaat tttatgttta aagtgagtgt
    45361 catatcacgg tgataacgcg aaacagctca caaaaataca cgcttacggg gtctttgcaa
    45421 cacaaaatag cgccgtttgg caaactgaag ggtttattgc tgaatgcctg ctcccctctg
    45481 gttcgagggg aacagaatgc gctgcttaga ggatttccag cagttcgact tcaaacacca
    45541 gggtgctgaa cggagggatg gatgcgcctg cgccccgctc gccatatgcc agttcctgcg
    45601 ggatagtcag ttcccattta gaacctaccg gcatcagagt cagtgcttca atccagccag
    45661 ggatcacgcc attaaccggg aattcagcgg gttcaccacg agcaacggag ctgtcaaaca
    45721 cggtgccgtc gatcagttta ccggtgtaat gaacacgaac gcggtcagta cgtgccggaa
    45781 ttgcgccttc accctggttg atcacgcgga attgcaggcc agattcggtg ctgttcacac
    45841 cttctttttt ggcattttct tcaagatatt tcacaccttc ggcagccatc gcctggaaac
    45901 gctgacgacg aacggcatcg gcgcgttcgt ggatttcacg cagcgcgcga tgcaccacat
    45961 caaccggaac agccggatgt ttgccttcca gcgcatctgc aataccagcc accagcgcct
    46021 ccggcagcag cccttgcagg ccagattcac tcagttgttg gcctacctgt aaaccaatgc
    46081 cgtagcttgc ttgcgcttcg atggtgtcaa aagttggggt ggtcatcgta tttcctttct
    46141 tagggtataa aagtagcggg cagcatatca gccacacttc tttgggtaaa actttgtcgc
    46201 aggaatgatg acaaatcccg gggaaaaaga aacaatagca atatcccgta tgaatataat
    46261 aaaggcgggt ttaccacgga catcatagcg ttagcttatt cataggctat gattgaggaa
    46321 caagacgcgg agcaggagga aaccatgccc gggcgctttg aactaaaacc aaccctggag
    46381 aaagtctggc acgcgccgga caattttcgc ttcatggacc cgctgccgcc gatgcatcgt
    46441 cgggggatca ttattgccgc cattatattg gtggtcggat ttctgcttcc atctgatgat
    46501 acgcccaacg cgccggtggt gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa
    46561 ccgccgacag aagaacagct gcgggcgcaa ttagtcacac cacaaaatga tcctgatcag
    46621 gtggctccgg tcgctccaga acctatacag gaaggccagc cagaagaaca gccgcaaact
    46681 acccaaacac aaccattcca gccagatagc ggaattgata atcaatggcg ctcttaccgt
    46741 gtcgaacccg gtaaaacgat ggcgcagctg ttccgcgacc acggactgcc cgctaccgat
    46801 gtctatgcaa tggcgcaggt agaaggcgcg ggtaagccgc tgagtaattt gcaaaacggg
    46861 caaatggtga aaatccgcca gaacgccagc ggcgtggtga cgggcttaac cattgatacg
    46921 gggaataatc aacaggtcct gttcacccgc cagccggatg gcagttttat tcgtgcgcgg
    46981 tagatgaagg gtcaaccaaa agccagctaa ggattgtctc tttgatgacc ttacgtctgg
    47041 cttccagtgc ctggggggaa accatactga tatgataaac caggccaagc gtatgctggt
    47101 tgatcaaata atatccgcca agcgcagcaa tattaatatt cacctgcaat gggtcgatat
    47161 ctggcttaaa aatattgtgt ttcttccctt catccaataa ggattccatt aactgcaagt
    47221 gcgcatggtt aatttctaag agacgctgtg attttgcata atgcacacct ttactttgat
    47281 tttcactatg cacaattttt aaaaaccagg gattagcaat ataataatcc catgtaaaat
    47341 caataatttt ctcaatggct ttttgtggcg gaagatctga gaaatcaagg gccttttctt
    47401 tttgtcgaat atcgttccag gtatattcca gtacttcaat aaataaattt tctttattac
    47461 caaagtgatg ataaaccgtc tgtttactac accccgcagc cttaacaata ttatcgaccc
    47521 tggccccttc ataaccatat tcggcaaatt cattaaccgc gcataacagc agcttttcct
    47581 tcaggctctg tacgttgctg gtatcaagat acataacgct tccccacgac ctcttcaaga
    47641 taactatctt aacccgatta attagaagct ccagtaaaaa ggataaaaac ttgtactttc
    47701 atcaagttat caaatgtcct gtgatcacta tcgcatatca accaaccaga tggttgatct
    47761 ttcttgcggc acgtcgcaag ctaattgtca cttagaaaac aatattcaaa aaaagagagc
    47821 tattgtatga aatccactcg tcctgttgcc gtgattaccg gagccgctcg tggtattgga
    47881 aagggctgtg cgctggagct tgctcgtggc ggatttaacc tgctgattaa cgatcttccc
    47941 gatgccgaca gcgtagaaaa actgcatatc acacaacaag aatgtatagc tgaaggcgtg
    48001 gaagtgattt gcttccctgc cgatgtcggc gatctctcct tgcatgaaga gatgctcgac
    48061 gcggcgcaaa acctgtgggg acgtctggac tgcctgctca acaacgcagg catttcagtg
    48121 aaaaaacgcg gtgacctcct cgacctcgaa ccagacagct tcgatcaaaa cattgccatt
    48181 aacacccgcg cgccgttctt ccttgctcag gcattcagta agcgtttact ggcacagccg
    48241 aaaccggaag cggaactgcc gcaccgctcg attattttcg tcagttccat caacgccatc
    48301 atgctggcga tgaatcgcgg cgaatacacc attgccaaaa ccgccgtttc tgccgctgct
    48361 cgtctatttg cagctcgcct ttgtaatgaa caaattggcg tttatgaagt gcgcccaggt
    48421 ctcatcaaaa ctgatatgac tattccggca acggcttatt acgacgaact gattgccaaa
    48481 ggtctggtgc cgtggggacg ttggggttat ccggctgata ttgcttccac tgtccgcgcg
    48541 atggcggaag gtaaactgat ttacacctgt ggtcaggcgg tcgccatcga cggcggctta
    48601 agtatgccgc gcttctgagg ctgaatcatg agagatctgt tacaacgccc ggatttattc
    48661 agtatcaata ccgcaacact gggctataaa acgccgctac ccgccattat tgatgcctgc
    48721 gccgcgcgtg gtattggcgc gattgctccc tggcgcaggg agttgcaagg tgaagatctc
    48781 cagcaaatcg cccaccagct tgccgccagt aacatgagcg tttccggttt gtgccgtagc
    48841 acttactaca ccgcgccgac actggcggaa cgcaaactgg cgattgatga taaccgccgg
    48901 gcgctggacg acgcagcggt gcttaacgcc gcctgttata tgcaggtggt tggcggtctg
    48961 ccaacgggca ctaaagatct atatgaagcg cgagaacaag tgaaacaagg gattcgccag
    49021 ttgctgccac attcgaaaga tgtcggcgtg ccaattgcgc tggagccact gcacccgatg
    49081 acagctgccg atcgctcttg cctgtgcacg ttacgccagg cgctggactg gtgtgatgaa
    49141 ctcgatcccg atggtgagtt tggcctcggc gtggcggtgg atgtttatca cgtctggtgg
    49201 gatccggatc tcgccagtca gatcctgcgt gccggaaaac gcatcctcgc gtttcatgtt
    49261 tccgactggt tagtgccgac cactgatctg gtcaatgacc gtggtatgcc gggagatggc
    49321 gtcatcaata ttccgtcaat tcgtcggctg gttgaaaacg ccggatttaa tggcgctatt
    49381 gaactggaaa tattctcgcc gtactggtgg caaaaagata ttaacagcac gctggatatt
    49441 agcgtcgatc gcatcgcgca ttattgttaa ggaatgatca tgagtgacca acccgttctg
    49501 ttcagcagag cggcggcttc ctgccgcctg acattgaacc gtgaagataa atgtcatgct
    49561 attaacgaag agatgatcga atctctcgac cattatttaa atgaaattga aaacgacacc
    49621 acattacgat tagtcgaatt aacggcaacc ggcgataaat tcttttgtgc gggcggcgat
    49681 attaaatcgt ggtcggccta ttcgccatta gatatgggca gaaaatggat taaacgcggt
    49741 aatgacgttt ttaatcgcct gcgaaactta ccccaattaa ccgttgcgaa tctcaacggt
    49801 cataccatcg gcggtggtat tgaactggcc ttgtgctgcg atattcgtat tgcccgcccc
    49861 ggcgcgaaat tctctaaccc ggaagtgatg ctcggcatgg tgcctggctg gatgggcatt
    49921 gaacgtgtgc ttaatcaggt tggtccggtg gtcggtcgcc agatgctgat gctgggtaaa
    49981 cgcctgacgg cgcaggaagc ccaggcggcg aatttaattg atgaagtggt agaaaaagaa
    50041 caggtggaaa gctggatggc gaaccagtta gcgcagctgg aaaaatgcgg cccggtggca
    50101 ctggcgcata ttaagcaact gattctggca ctggaaaata aacatgccga ttacccgcac
    50161 cagctactgg ccggattaat gtctgccact caggattgcc agcaggcgac gcgcgccttt
    50221 gccgaaaaaa gtagcgtgag cttccataat caataaggaa attgtatgcg taagataaca
    50281 accgccgagg cactggcggc gcaaattcag gacggtgcga ctattgctat tagcggtaac
    50341 ggcggcggta tggtggaagc cgaccatatt ctggctgcta ttgaagcgcg tttcctgcaa
    50401 accggacacc ctcgcgacct gacgctaatc cactcgctgg gtattggcga tcgcgactgc
    50461 aaaggcacta atcgttttgc gcacgcagag atgctcaaac gcattattgc cgggcacttt
    50521 acctggtcgc ccaaaatgca ggcgctggta aaaaataaca cgattgaagc ctactgcttt
    50581 cctggtggcg tgattcaggc actgctacgg gagatcggcg ccggacgtcc ggggcttttt
    50641 acccacgttg ggctgggatc gtttgttgat ccacgcaatg gcggcggtaa gtcgaatgaa
    50701 tgcactaccg acgagctggt agaactgatt gaaatcgacg gtgaaaccaa acttcgttat
    50761 cgccctttca aagtggatta cgcgattttg cgtggcactt atgccgatcc tcgaggcaac
    50821 gtcagccttg aagaagaagc gattgatatg gatagctatt ccatggcgct ggcagcacac
    50881 aacagcggcg gcaaagtgtt cgtacaggta cgcgatgtgc tggaagctgg cgcgattgaa
    50941 ccacgccggg tcaaattacc gggcattctg gttgatggca tcgttgaaca ccgcgaacaa
    51001 ccacaaacct atcttggcgg ttacgacctg accatcagcg gccaacatcg ccgtctgagt
    51061 tctaacgacg ctattgaact ggttagtcat ccggtacgtc gcctgattgc ccgtcgggca
    51121 gcacgcgaac tggtagctgg cgcttcaacc aactttggct ttggtattcc cggcggcatt
    51181 ccaggagtag cgctgcgcga aggcgttcct taccaaagtt tatggctgag cgtcgagcaa
    51241 ggcgtacata acggcatgat gctggatgat gcgtttttcg gctgcgcccg caacgccgat
    51301 gccattattc catcactcga tcaatttgag ttctacagtg gcggagggat cgatatcacc
    51361 ttcctcggca tgggagagat ggatcagtac ggtaacgtca acgtctccca tctcaatggc
    51421 aatctgattg gccccggcgg atttctcgaa attgcgcaaa atgcccgtaa agtggtgttc
    51481 tgcggcacgt tcgacgctaa aggcagcaag atcgatataa caccagatgg cttgcatatc
    51541 gcccagtcag gtcaaatccc taaactggtt accaaggtgg aaaaaatcac ttttagcgcc
    51601 gcctacgcac agcaaagtgg tcaggaagtg ttgtatatca ctgaacgcgc agtattccag
    51661 ttaacggcag aaggcgttga attaattgaa atcgcgccgg gtgtggagat tgagcgcgac
    51721 attctgccgt atatggcttt ccgtccaatt atcaagcacc cacgcctgat ggaaagtagc
    51781 ctgtttacgc cgatgaagga tgcatgagtc agcttgatga caccatcctt gatgcgctga
    51841 cgcacgttac tttcccaaag ggttttgcac aggcagagcc cgcatgggtt gtcacggtag
    51901 acggtgttga ttacccactc tggcaaacag atgccctggt agtcggcagt ggtgcagccg
    51961 ggctgcgtgc agctgtggaa cttaaacgcc gccagcaaaa tgtgctgatc gccaccgccg
    52021 ggttatatat ggggacgtcg gcatgttctg gttccgataa acagacactg tttaccgccg
    52081 ctaccgcggg caacggcgac aacttcacca aactggcaga ggcactggcg agcggtgggg
    52141 cgatggatca cgacaccgct tatgtcgaag cggtaggttc tctgcacact cttggcgggt
    52201 tgcaatatct tggtctggaa ttatcggaag atcgctatgg cgcgattctt cgttatcaaa
    52261 ccgaccatga cgaagccggg cgtgcaacct cgtgtgggcc gcgtacctca aggttaatgg
    52321 tgaaagtgct gttggaagaa gtacagcgcc tcgccattcc agtgttgacc agtgcaacag
    52381 tgattaaact gctgcatcag cgtgacgaaa acggcgaaga ccgtgttgcg ggggcaatcc
    52441 tcgcgaccgg tcatcgcgcc cataaccctt gggggctggc aattgtgact gcgcccaatg
    52501 tggtactggc aacaggaggg cctggcgagc tttatcgcga cagtgtgtac ccacacaaat
    52561 gttttggctc gctggggctg gcgctggagg aaggcctgac gctaaccaat ctgaccgaaa
    52621 gccagtttgg tataggcacg ccgcgtagca cgtttccgtg gaatttatcc ggcacctatg
    52681 tacaggtgat cccgtatatc tattccgtgg atgctgaggg taacgagtat aacttcctcg
    52741 cggattacta tcgcaccacc caggagctgg cttcaaacat tttccgtaaa ggctatcagt
    52801 ggccgttcca cgccactcgg gtgatggatt ttggctccag cttgttagat atggcagtag
    52861 cgcaagagca gcaatcaggg cgtcaggtat ttatggattt caatcgcaat cctgaagcgg
    52921 tgccgggtga cctgccgttc tcattagatc gactggacga cgacgttcgc gcgtatctgg
    52981 aaaataacga cgctctggca ccatcgccca tcgaacggct gcaacgaatg aatccgctgt
    53041 ctatctcgct gtataagatg cacggttacg atctcaccac acagcctttg cagtttgcca
    53101 tgaataatca gcatatgaat ggcggcattg aagtggatat ctggggacaa acctccctgc
    53161 ccggctgttt cgccgtgggg gaagttgctg gcacacacgg cgtcactcgc cctggtggtg
    53221 cagcattgaa tgccgggcag gtttttgctg ttcgtctggc acgttttatt ggttgcacgc
    53281 aaaagcggaa tattgatgga gatatagcac agctggtagc tcctacactg gcttctataa
    53341 gagagatcat tactcaggcg cacgataacg ggaccgggat gccgttgtcg gttgtgagag
    53401 aaaaaattca ggcacgaatg tctgaccatg cgggatttat ttgccatgcc gataaagtcc
    53461 gacgcgccac tcgcgatgcc ctgctattga gcgaatttgt ccaacggcat ggattggcta
    53521 tcaaacatgt gggtgaagtt gccgagctgt ttatgtggcg gcatatggca ctgacttctg
    53581 ccgccgtatt aacgcaatta acgcattata ttgacgcagg tggtggtagt cggggagcac
    53641 gtattattct cgacagggat ggaaattcta ttccgcaaac acgaaatggt ttttgtgatg
    53701 cctggcgctt tcgttcagag cgtactgagg ataaaaaaga taaactactt attcattatt
    53761 gcaacggtat ctttcacgtc agagaaacac ctgttcgtga gtttcctatt atccgaggta
    53821 tttggtttga aaaaaactgg cctggttttc ttaatgggac aatttaccag ccacaggatg
    53881 aatgatattg caaaggttaa tcactatgcg aaatataatt agatctatta atgctctgct
    53941 tgcagcattg aacattacca ttttggcgat catcgtggca tgtgtgacgt ggcaggtggc
    54001 tgcacgattt atatttacta gtcccagtat ttttacagat gaactcagcc gtttgctctt
    54061 tatttgtctt ggactttttg gtggggcata caccgcaggg caaaaccggc atctggcgat
    54121 tgacctgcta cctatgatgc tgaaaggaaa agcccgacgt catttatttt tgtgtattca
    54181 gattatcgtt attatcttcg caaccatcat tatggtatat ggtagtgggc tattaacgat
    54241 ggatacattc gattcaggtc agacctcgcc cgctttaggc tggcaaatgg gatatatcta
    54301 tatgagcatc ccgattagtg gtgtacttat tattatatac accatagata tggttttaac
    54361 cgaacttaaa caaccactct aaagataaca aacaacaacc aaccatagta ggaatggaga
    54421 aattatggat tactggttac cgattattgt gttatttggt gccttctttt ttatgttggc
    54481 attaggtgta cctattgttt atgccattgg tttatcgaca ctggcatcaa tttccacaca
    54541 actcgatttc aactcagcat tgagtgtggt ttcccaaaag ttagcctccg ggttagatag
    54601 ttttacgttg ttggcaatcc cattttttat cttgtctggc aacattatga accatggagg
    54661 aatagcgcga cgattaatca actttgcccg tattctcggc ggtcgtctgc caggctcact
    54721 tgcccactgt aatattctcg ccaatatgct gttcggggca atttctggtt cagccgtggc
    54781 ttcggcggct gcgatgggcg gcgtcatgca tccgcaacag gttaaagagg gttacgaccc
    54841 ggcattcagt actgcggtta acgttgcctc tgccccgacg ggtttgctta ttccaccaag
    54901 taatacgtta atcgtctatt cactggtcag tggtggtact tctattgccg cactgttcct
    54961 ggcgggttat gttccgggaa ttttgctggg tctggcactg atggtaatag ccggtattat
    55021 cgcggtacgt cgcggttatc caaagccaga acgcccgact ttacgccagg ctggcgttgc
    55081 gatatggatg gctatcccca gtattttcct gattatttta attatggggg gtgtattaag
    55141 tggtattttt accccgacag aggcctccgc aattgcagta atttataccc ttttccttgc
    55201 actggttctt tatcgtgaaa tctcagttaa agatttacct aaaatatttc tggaatccgt
    55261 tattaccact gcaattgtct tgttattaat tggttcttcg atgggtatgt catgggcaat
    55321 gtcaaacgct gacgttccct tcctgattct cgatttgctt aacactattt ctgacaaccc
    55381 aataataatc ctgcttatta tcaatattat cctgttgatt atcggcactt ttatggatat
    55441 gacaccggcg gtactgattt ttacgccgat atttttaccg gttgtcaccg aactgggcat
    55501 ggacccgatt cactttggta ttgtcatggt actgaacatg tgtatcggta tttgtactcc
    55561 gcctgtaggc agtgtgttat ttgtcggttg tagtgtatct aaattaccga ttaataaaat
    55621 tatcaaacct atgctgccat tctatgccgt gatggtattg gtgctggcaa tggtgaccta
    55681 tattccgcaa atcagtatgg cattgccaag agcactgggc tactgatgcg cattcactgg
    55741 cggcacctgc cgccatattt attagaaaag gaacatctat gaaaataaaa gtatccgctg
    55801 gcattatcgg tgctgttctt atgttatccg caagccagtc ctgggcagtg acattaaaac
    55861 tgagtcataa tcaggataag tctcatcctg ttcataaagc gatggagttc tttgcgaaaa
    55921 agagcaaaga gtactctaac ggtgatatta ctattcgtat ttatccaaat ggaacattgg
    55981 gtactcaacg agaaacaatg gagctgattc gttctggcgc tattcccctg gtaaaaacca
    56041 atgcggcaga aatggaagca tttgaaaatt cctataaatt atttagcctg ccttatttgt
    56101 tccgcgatcg tgatcattat tatcaggtca tgcagggcga tatcgggaga aaaatcctcg
    56161 actcaacgaa aagcaaaggt tatttcgggc tgacttttta tgatggaggc gcccgcagtt
    56221 tctatggcaa taaaccagta ctgaaaccag acgatctgaa aggcatgaaa gtccgtgtcc
    56281 agccaagccc tggcgcagtt gaaatgatca aagtcatggg cggtaacccg acgccactgg
    56341 attacggcga gttgtataca gccttacagc agggtgtggt cgatatggca gaaaacagcg
    56401 tgatggcgct gactaccatg cgtcacggtg aagtggcaaa atccttcagc cttgacgaac
    56461 acactatggt tcccgatgtg gttctgatga gcaatgctgc gtttgataaa cttagccagg
    56521 aaaatcaggc agttatatta aaagcagcta aagaatcaat gagctacatg aaagacttgt
    56581 ggagcgagga agagaaacaa gaatttgcaa aactggataa aatgggcgtg aaagtctacc
    56641 aggtagataa agctccgttt atcgagaaag tacagccaat gtacgcaaac ttcgctaagg
    56701 acaacccagc ccttgcccca atgctggctg atattcaggc agctaagtaa ttatcttcgt
    56761 agggctgctt attgtggccc ttatcgatat ctgcaagtgc tcgctacaaa agcaaaacgc
    56821 cgaccaatgg tcggcgtttt tacgtctcgt tgaataacga attattcagc tactacgttt
    56881 acgatcactt tcgcgaatac ttcgctgtga acctggaagc tcacttcgtg ttcgccagtg
    56941 gtacgcagaa cgccgttcgg cagacgaact tcgctcttag ccacttcaac gccagctgca
    57001 gttacagcgt cagcgatgtc gcgagtaccg atggaaccga acagtttacc ttcgtcgcca
    57061 gctttagacg cgatggtaac agtttccagt gcattgattt tctcagcgcg agcattagca
    57121 gctgccagaa cttcagccag tttagcttcc agttcagcgc gacgtgcttc gaagaattca
    57181 atgtttttct tggtagctgg aacagcttta ccctgcggta ccaggaagtt acgagcatag
    57241 cccgctttaa cgtttacctg atcacccagg ctacccaggt ttgctacttt atcaagcaga
    57301 ataacttgca ttaccttatc ctctcaaagt cgtattaatg gaccgtgacc gattactgat
    57361 ggcgatcagt gtacggcagc agggacaggt agcgagcgcg tttgatagcg cgagccagct
    57421 gacgctggta ttttgcacgg gtaccggtga tacggcttgg gacaatctta ccgctttcgg
    57481 tgatgtagtt tttcagcgta gcgatatctt tatagtcgat ctcttgaacg ccttccgcgg
    57541 tgaaacggca gaacttgcga cgacggaaat aacgtgccat atggctagtc tccagaatct
    57601 atcaattcaa tctgctcggc atgcaaaacc attttgctca gtccgttctt tgccttgtgg
    57661 catgaaatga acccctgaac ggttatgcga ctgccgaccg ttatactgtg agtaatggcc
    57721 tggttttcgt gtccgctaac aataacgggc atttgacacc atgcctgccg gtgaaaaccg
    57781 gcttcctcct gcacagaacg atgctcaagc acgaactggc agtgaggaat tcctgatgga
    57841 ctgacctttc gaaggggagt cctgcacacg gtgccggaca acaccagacg gttggtcatc
    57901 agaaattact cttcagaatc cccagcatct gcatcatctg cggtttcgtt tgcgaaatca
    57961 tcgcgacgct cgcggcgctc gtctttcgct ttaaccatcg gagatgcttc ggtaacagcg
    58021 tgcttggtac gcataaccat gctgcgaata acggcatcgt tgaagcggaa ggtagtttcc
    58081 agctcatcga tcacttcctg cggagcttca acgttcatca gaacgtagtg tgctttgtgc
    58141 agtttgttga tcgggtaagc cagctgacgg cggccccagt cttccagacg gtggatcttg
    58201 ccttctgcac cagtgatggc agcagtgtag cgctcgatca tgcccggaac ctgttcgctc
    58261 tggtcaggat ggaccataaa aacgatttcg taatgacgca tcgaattgct ccttacggat
    58321 tattcagcct cctgtctggg tcagccgaat cccggggagg caaggaacgt gttaaaggtc
    58381 ggctgaaaaa tgacgcgtta tagtactgac ataccttgct aaactcaagg gcagagcaga
    58441 aataaatctc ccctttcgca aataaccact aagccgctga tttgaagttc atcacacttc
    58501 atcatgctgt ttttttgaac aactgcatca gaaatggtcg ccatcaactt tttcatccgt
    58561 gattaaaatt aaatcagacc atcaccacat gaggagagca cacaaaaaca gccgtcgagg
    58621 aggtatctat gttcagccgt gttttagccc ttctggctgt gcttttgcta agtgcaaata
    58681 catgggcagc cattgaaatt aataaccacc aggccagaaa tatggacgat gtgcaaagct
    58741 taggcgtgat ttatatcaat cataatttcg ccactgaaag cgaagcgcgt caggcattaa
    58801 atgaagagac agatgcgcag ggcgcaacgt actaccacgt gattctgatg cgggaaccgg
    58861 ggagtaacgg caatatgcac gccagcgcgg atatttatcg ctagcaccag gtataaccaa
    58921 agaaacattg ccatagtttg ctttgccccc ttctcagggg gctttttttg aaaaacggct
    58981 gcggaattag accagttatc tcccgaggaa ggaaatttcc gcagcgcgtg ttctacttct
    59041 gcccgtaata agcgttagga ccgtgtttac gcatgaagtg tttattcatc aggaagctgt
    59101 cgatgtgatt gagttgtgga ttaatactgc gcgcaatcca cgccattttc gccacttctt
    59161 ccatcactac cgcgttatgc accgcgtcgt gggcatcttt cccccaggcg aacggtccgt
    59221 gctgatacac cacaattccc ggcgtatgca gcggctcggc gttgcccagc gtttcgataa
    59281 tcactttgcc agtgttcagt tcgtactcac cctgcacctc ttcttcgctt aatccgcgcg
    59341 tgcacgggat atcaccaaag aagtagtcag cgtgcgtggt gcctaacgcc gggatcgcca
    59401 gccccgcctg cgcccatgcg gtggcatgag tggagtgggt atggacaatg ccgccaagcg
    59461 acgggtaacg acggtagagt tcgagatgcg tcgcggtgtc ggaagatggg cgatactccc
    59521 cttccaccac cttgccgctc atatcaacca ccaccatatc ggccgctttc atggtttcgt
    59581 aggcaacgcc gctgggcttg atcaccacca gcccgcgttc gcggtcgata gcgctgacgt
    59641 tgccccaggt aaaggtcacc agcccgtagc gcggcagctc catgttggct tcaaatacct
    59701 gctgttttag cttttgcatt atgccgcctc caccatgccc gctttcgcca tgcgcgcttt
    59761 cacccaatca cgcgctttcg ccacttctgc cgccgggtct tccgccgttt cgctccacat
    59821 ctcaatcagg tacggcccgc aatagccact ctgtttgagc gtttcgaaac aacgttcgaa
    59881 atccaccacg ccttcgccaa acggcacgtt tttgaagacg cccggtttgg tgtctttcac
    59941 atgtaccgcg acgatatgcc cgattccggc ctgcaactcc atctgcacgt cgttgtccca
    60001 cgccgagagg ttgccgatat ccgggtagag ctggaaccac gggttgttga gatagtgcgc
    60061 gtagcccagc gccttgctga tggagttcat caatgggtaa tccatgatct ccatcgccag
    60121 cgtcacctgc gcgcggcttg ccatctcaac gctctctttc aggccgtcac ggaaacgacg
    60181 acgcgtttcg ttattggctt cctgatagta aacgtcatag cccgccagtt ggatcacgcg
    60241 aataccgaca tcctgggcga actggatagc tttacgcata atctccagcc cctgcgcccg
    60301 caccgcgtca tcttcactgc ccagcgggaa acgacgatga gcagaaaggc acatggacgg
    60361 cacgcgcacg ccggtttcaa caatcgcgtt caccagcgcc agacgctgct cgcggctcca
    60421 gtcgaggcgc gacaggcgct cgtccgtttc atctaccgac atctcgacaa aatcgaagcc
    60481 caacgttttt gccagttgca ggcgttccag ccagcactcc ccggcgggga gcgctttttc
    60541 atagatgcca agcgggattt gtttggacaa catatccgct ccttagcccc acagttcggc
    60601 gatggaacgt ttgaactgac gtgcagcttc aaccggagaa gcggcatcac ggatgctgcg
    60661 accagcgata aacacatgaa tcggaatacc tttgaacagc ggcagatctt ccagcgccag
    60721 gccaccggtg acggtgactt tgaagcccat atcggaaaga cgtttgatcg cggtaatgtc
    60781 cgcttcgccc cacgctacgc ctgcggcctg cgcgtcacgg ctgcggtgat aaaccacctg
    60841 ctgaatgcct gcgtcacgcc actgctgcgc ctgttcccag gtccagtagc cggtcagttc
    60901 gatctgcacg tcgccgttaa actcttttgc tacgtccagc gcgcctttgg cggtgttgat
    60961 atccgcacag caaattaccg tcacccagtc agcgttggct tcgaagcaca tacgagagag
    61021 gattttgccc gcatcggcaa ttttggcgtc tgccagtacg attttgtgcg ggtaaagcgc
    61081 tttcagatcg cgaaccgcac gcacgccttc gcccacgcac agaatggtac ccacttcgat
    61141 aatgtcgact tcttcggcaa tcaggcgagt ggtttcgtag gcgctatcca tagtctggtt
    61201 gtccagcgcg acttgtaaca tcggtaatga catcttcaat tccttcttaa gctgccgcgt
    61261 tggtgcggtc aatgagatcc agtacttcct gctcggtacg gcaggcacgt aaacggtcga
    61321 aattttcttc atcttcaaac aggttgacga tctgcatgat gcccacttcc tggtgcgtgt
    61381 tggcatcgac cgccgccatg gtgatgagga tatccaccgg gtcgttatct tcgtggttga
    61441 actccagagg ctttttcagc gtcaccagcg caaaaccggt tttcttaacg ccctcttccg
    61501 ggcgaccgtg cggcattgcc aggcctggtg cgagcacaaa gtacggaccg tgctgttcaa
    61561 ccgcatccag aatcgcctgg tagtaacgcg gctcgacaac atccgccgcc accagcaggt
    61621 caacgccgat ttttactgcg tcctgccatg tctcagcttc agcctgcagg cggatggatt
    61681 tattctccgc cagcgaatca cgtaatttca tggcgcgtcc ttacttcaca tcctgcggga
    61741 aatgctcttt gatcacttcc agcagtttcg ggccaaagtc ggcaggagag agcatgttgc
    61801 gcacaccaac cacgtatttg ttgccggtca cggtgatttc gcccgcaatg tgcgtagaag
    61861 cgatgatgat atccgcgccg ctcaactcgc ttttgtattc gccaaccgcg cagctgttta
    61921 ccgtgtggtc aatgtttgat tgggttaaaa actggtccac tttcatcttc atgatcatgg
    61981 aactgccttg tccgttgcca cacacagcca gaatacgtac ggtcataatc aaaactcctt
    62041 attaagcaga ctgttctgcc agttgttttt ctgcatcttc ttctgcgcgc agtgcgcggc
    62101 cagcgaagaa catataagcc agtgcaatta caatgatgac ggccataaag gcgataccga
    62161 tggagaagaa gccctgcatc atcggcggtg ccagaatcga ccagtccgcc atacccatcc
    62221 aggcactcat accggtaagt ttcaccgccc agacgcaacc aaagatttcg atcatcccca
    62281 tcaccagaca aatcttcagc gccgcacgcc agccgccgaa gtggttagcg aacacgccga
    62341 tggtggcgtt ggagaagaac atcgggataa agccaggaat aatcaggatc gaggaaccgc
    62401 aggcgaccag gatgccaacc gcaatcagct gaccgatagt gccccacata aagccccaga
    62461 ccacggcgtt cggcgcgaag ctataaatag ccgcacagtc aatcgccaga accgcacctg
    62521 ggatcaggcg ctgggaaatg ccgttaaatg cttcagagag ttccgccaca aacatgcgca
    62581 cgccctgagt gatgatgaag atcgccaccg caaaggagaa accagtttgc aggatgtaca
    62641 ccgtccagtt cactttgcct gccatcgcct gcacggtgtc gataccgaag gagagcagaa
    62701 tggcaccaaa gaagatggtc atgacaatcg ccgtggagac gatgttgtcg tggaaaatat
    62761 tcagccagcc tggcagtttg aggtcttcaa cgctttcctc ttttttgccg aggaacggcg
    62821 caactttata ggcaatccat gatgcaaact gctgctggtg accgatggag aaaccgcagc
    62881 cgtccgtcac ttcctgagtc ggcttgtaca tcatgttgga ggtaatgccc cagtagagcg
    62941 aaaccagaat cgccgtgcag ataatggttg tccacatgga gtagccgaaa ataaacaacg
    63001 taacggcaat cagccccgcc tgctggaaca tgatatgacc agtcagcatg atggtacgaa
    63061 taccggtaat gcgacgcagc agcacgtaac agatgttcag cgccagcgct aacaacaccg
    63121 cgtaacctac ccagctatag gcatcgccca tgcggtcgat ggttgccatc atcgacgcgt
    63181 aagtatcgga aattgcgccg ttaatgccgt agacttcgga cattttcgcc accaccggtt
    63241 tgaaggtgct ggtgaggatg ccggaccctg cctgcaacaa catgaaacca attatagttt
    63301 taatcgtgcc tttaataata acgctgacac ttttgcgcag taggatgtag cccagacagg
    63361 tcacaatacc cagcaacaac ggggcattgg tcatgacctg gttaaaaaac acggtaaaga
    63421 tgttgtagag gatctccata acgatctcct gaagaagagg taaccgggta ttccgcacac
    63481 ccggaatgtt gtgcactcac tctaattttc aaaagtaatc acaacaagat tatttatgat
    63541 taaatgtgac gcgacccgaa aataattcca cacggataga gtgggattca cataacgaaa
    63601 caattattta tttaaattca tttagttaat ttaactacac attccattaa atctttccac
    63661 actcaaaatc aacaacaact tccctggcaa aaagtcaaaa aatggtcatt gccttcctga
    63721 aaaaatagtg tcaggattaa tcaaaaccaa tcacatattg attcgaattg aactatcatg
    63781 aaggtaaatg gcgatgagta aagtgaaaag tatcacccgt gaatcctgga tcctgagcac
    63841 tttcccggag tggggtagct ggttgaatga agaaattgaa caagaacagg tcgctcctgg
    63901 cacatttgcg atgtggtggc ttggctgcac cgggatctgg ttaaaatcgg aaggtggcac
    63961 caacgtttgc gttgatttct ggtgcggcac tggcaaacaa agtcacggta acccgttaat
    64021 gaaacagggt caccaaatgc agcgcatggc tggcgtgaaa aaactgcagc caaacctgcg
    64081 caccaccccg tttgttcttg atccgtttgc gattcgccag gtcgacgcgg tactggcgac
    64141 tcacgatcac aacgatcata tcgacgtcaa cgtcgctgct gctgtgatgc agaactgtgc
    64201 tgatgacgta ccgttcattg gaccgaaaac ctgtgtggat ttgtggattg gctggggcgt
    64261 accgaaagag cgttgcatcg tggtcaaacc gggcgatgtt gtaaaagtga aagacattga
    64321 aattcatgcg cttgacgctt tcgaccgtac tgcactgatc accctgcctg ccgatcaaaa
    64381 agcggctggc gtactgccgg acggcatgga cgatcgcgcg gtgaactatc tgttcaaaac
    64441 gcctggcggc tccctgtatc acagcggcga ctcccactac tctaactatt atgcgaagca
    64501 cggtaacgaa catcagatcg acgtggcttt aggttcatac ggcgaaaacc cgcgcggtat
    64561 caccgacaaa atgaccagcg ccgatatgct gcgtatgggt gaagccctga atgcgaaagt
    64621 ggttatcccg ttccaccacg atatctggtc aaacttccag gccgatccgc aagagatccg
    64681 cgtgctgtgg gagatgaaaa aagatcgcct gaagtatggc ttcaagccgt ttatctggca
    64741 ggttggcggc aaatttacct ggccgttgga taaagacaac ttcgagtacc actatccgcg
    64801 tggtttcgat gattgcttca ctattgaacc ggatctgccg ttcaagtcat tcctgtaatc
    64861 tgtcgtttat accggatagc gtggagtagt tcgcgctatc cggcgtagta tttcctggct
    64921 atttcaaata tcatctaaaa aaatcaaatt ttatcggaat agctcatgac tgaagcacaa
    64981 agacatcaaa tcctcctgga aatgctcgca caattgggct ttgtgaccgt tgagaaagtc
    65041 gttgagcgtc tgggaatttc gcctgccact gcgcgacgcg atatcaataa acttgacgaa
    65101 agcggcaaac tgaaaaaagt gcgcaatggc gcagaagcta ttacccaaca gcgcccgcgc
    65161 tggacgccga tgaatctgca tcaggcgcag aatcacgatg aaaaagtacg tatcgctaaa
    65221 gcggcctcgc agctggttaa tccgggcgaa agcgtagtca tcaactgcgg ctccaccgcg
    65281 tttctgcttg ggcgggaaat gtgtggcaag ccagtgcaaa tcatcactaa ttatctaccg
    65341 ctggcaaatt acctgatcga tcaagaacat gacagcgtga tcattatggg cggacagtac
    65401 aacaaaagtc agtccatcac tttaagcccg cagggcagcg aaaacagtct ctatgccggg
    65461 cactggatgt ttaccagcgg caaaggactg acagcagaag ggttgtataa aaccgatatg
    65521 ctgacggcaa tggcagagca gaagatgctg agcgtggtag ggaaactggt ggtactggtt
    65581 gatagcagta agattggcga acgcgcggga atgcttttta gccgtgccga tcaaattgat
    65641 atgctgatca ccggcaaaaa tgctaacccg gaaatcctgc aacaactgga agcgcaaggg
    65701 gtcagcattc tgcgtgttta aagatgctgg cggaaaaatg tcactgcagc atctaacgcc
    65761 tcaggcgtaa tgcggtgacg cacgcctggc tgccatgaac aggttagcag tttatcccgt
    65821 cccgtctcgc ttaaggcctg ctgcaaacgt agtgattcat cggcaggcac aacgtcatcg
    65881 tcgaggccat gccacagcag tagaggtctg tcaccaagtt gttccaggtg gtttgtcgct
    65941 tcccactctg ccagcggcgc gacaatgtta ttgaattcat tctgttgtgc tgccgtttca
    66001 ggtatcagcg gtggaaacag tgaacgggcg agcgatgtaa aatagcccga tcccatcatg
    66061 ctggcggtac atctcaccgt ggggtggcga gcggtaatcc ccagtgccgt catcgcgccc
    66121 atcgacgcac caccgactgc cagacggtca tcaagcagcc acttttcttc ggctattgcc
    66181 gcacgtaaag tagtgaattc ctgcatactt tgtagcaaga tttgccagaa ttgatttaac
    66241 cgccgcgctg cgtcgccact aaaacgacta ccgtgatcgg gcgcatccgg catgatcacc
    66301 cgcaaaccag cctgcgccag cgcaacggca aaatagctat acaccagact ggacgaagta
    66361 aagccgtgat aaaaaatcgc gcacggtaac ggggtatctt tttgtccgac aggataagca
    66421 tgaagaacgg gaatatctgc cagctcgcgt gattctattt caatcatctg tctctccttt
    66481 ttgtctggta tgacatgaag aaatccttag cacaagtttc tgctggcggt attagttcat
    66541 aatgttgaga tgtgggttac gctttcgtca cgttttcatt cgaaaatcgc gtaccaggta
    66601 acaattcggg aacattcccc caaagttaaa ttaataaggc actacactat ggttagcagg
    66661 aaacgtaata gcgttattta ccggtttgcc agtttattat tggtgttgat gttaagtgcc
    66721 tgtagcgcac tgcaaggtac gccacagcca gcaccgccag taacggacca tccgcaagag
    66781 attcgccgcg accagacgca agggttacag cgaataggta gcgtaagcac gatggttcgg
    66841 ggttctccgg atgacgcatt agcagaaatc aaagcgaaag ctgtcgctgc aaaagctgat
    66901 tattacgttg tcgtcatggt ggacgaaacc atcgtgacag gacagtggta ttcacaagcc
    66961 attttgtacc gtaaataaat cagacaactt acatagtcag ctttacattg ctttgcgtcc
    67021 ctatgcgttt acctgtgcac aataaattac cggctggaag cctgggcgtt tattaccgcg
    67081 atggaatgcc ctgcaacatg tggggaaacg aaaaatggag ctgacgatga aacaattact
    67141 tgcctcaccc tcgctgcaat tagtgactta tcctgcgagc gccacggcac agtctgcaga
    67201 attcgccagt gctgactgcg tgacgggcct gaatgaaata ggtcagatct cagttagcaa
    67261 tatttcaggg gatccgcagg acgtggaacg tatcgtggcg ttaaaggccg atgaacaagg
    67321 tgcgtcatgg taccgcatta ttaccatgta cgaagaccag cagcctgata actggcgcgt
    67381 acaggcgata ctgtatgcat gagtgtcatc tacagcaaat gaacaatgcc ggatgcggcg
    67441 cgagcgcctt gtccggccta caattttcac atatttcatt tagttaatcg aaaccagcgt
    67501 cgcatcagtc gtatacgctt acacacacac tccccccgtc gcccgcagca ataaatcatt
    67561 ctggatctgc tctgacaacc gtacgccgcc gcgcgtatct aacatcacct gacaccacgc
    67621 ctgcgccatt ggcggagaag catatttcaa catttgcgca ccgcagccca gcaggaatag
    67681 ctgatgagta atctctcgcc ccagttcttc agctggctta cgcagctgct gctgtaaacg
    67741 acgaaccgcg cgatcaaaat agcgatcctg ccctttcact tccacaaatg cttccgacaa
    67801 taagtcgtat acgcccgctt gcttattgag aacgcgcaac acatccaggc acataatatt
    67861 gccggaacct tcccaaatac tgtttaccgg catctcccgg taaagccgcg gcagctcgct
    67921 ctcctcgcaa taaccaatgc cgcccagcac ctccatcgct tcggccacaa acggcatacc
    67981 tcttttgcag atcacaaatt ttgccgcagg cgtaaataaa cgcgcccaca gggcttcttt
    68041 ggcatcggca cgccggtccc acgctcgcgc aagacgaaac agcaacgccg tttgcccttc
    68101 aagctgaagt gccatgcgac ttaagacatg acgcataagg ggctgttgga tcaatggatt
    68161 accaaaaaca tggcgttgat gtgcatgata aatcgccagc gaaaatgcac ggcgcatcat
    68221 ggcatggcta cccagggcgc aatcaaaacg cgtcatccca cccattttca ggatcagacg
    68281 aattccttcc ccttcctgcc ccaacaacca accaatggca tcctgaaact ccacttcgca
    68341 actggcgtta gagcgattac ccagcttatc tttcagccgc tcgaggcgaa tcgcgttgcg
    68401 ttgcccgtca ggcaaaaagc gcggcacaaa aaagcaggac agaccacccg cggtctgcgc
    68461 cagcaccaga tgcgcatcgc tttgcggaac cgagaaaaac catttatgcc ccaccagccg
    68521 ataagagcca tcttccagac gctctgcacg ggtggtgttg ctcataacat cggaaccgcc
    68581 ctgcttttcc gtcattccca tgccaatcaa caaaccgcgt ttttgcccac ctggcaataa
    68641 gtgagaatcg tagcgatcgc tcaatagcgg cgtggtccag tcctgaaacg gcgcgggtaa
    68701 catctgtaac aacaatggcg tggcggcaaa ggtcatggtt atcggacata acgaccctgc
    68761 ctcaacctgc gcatgtaaca taaaacgcgc cgcgcgcgcc acaaatgcgc cggagcgagc
    68821 gtcttcttcc caggcaagat tgtgcacccg attggtacat agcgcctgca tcagcaggtg
    68881 ccaggcgggg tgaaaacgca catcgtccag acggcgtcct tgcgcatcgt agcgcaataa
    68941 ttcaggcgga ttcacattcg ccagccgccc cagttcaagg gattcagccg ttcctaactg
    69001 ctgaccaata ctggctagaa aatcgctatc ccagccagca ccttcacgcg ttaccgcttc
    69061 gcagagcgcg ccatcagaca ggtataagtt gctgttattt aatggtatag gttgattaaa
    69121 aacggtgtga gtttgccagt gcactgtgtc tccctccatc aatggcagtc accattagta
    69181 tggtcactgc catggattca tgacagaaag agattgctaa aacattctta tccagcaatg
    69241 taatgcggaa cgaaacgtga ggtgtctttg gtgatcagtg tgttatcttc acggatcccc
    69301 attccgcacg cttcatcatc gacaatccag ctaccgatga gtgtgtagct atcgccaaac
    69361 cgcggcagag gttgaaacgc ctggtagatc atcggttcat cggcgtaatc accatcagcg
    69421 tggtcaacga cgttattctg gccgtcgaaa atggtgacat ttccgccttc gcgcgagtag
    69481 agcggtttac gcacatagct ttcgccagcg gcaatctgcg atttttcacc ctcaaaccag
    69541 gatgcaagaa ggttaggatg accagggaag aagcgccaaa gcagcggcat tagcccttta
    69601 ttactcaaga tacttttcca taacggctcc acccattgct cgcgacgctt gcgcagcagc
    69661 ggaccgttat cgtcacgcat catccactcc agcggataca gcttaaatgc acgctggatg
    69721 acattatcat caagatcggt cagtacgccg ccgacgccca aaccgagatc ttcaatgtag
    69781 ataaaccgcg actcctgccc tgcctgctgg gcgcagtcct gcaagtacag cacggtactc
    69841 ctgtcttcat cggtgtcctg acagcagcaa aaataaaacg gttcccgact gtaaagctcg
    69901 ctaaagcgtg aaatcaggcg ttcctgaata gcattgtact gatcggcatc acgcggaata
    69961 atgccgctgc gccgggcatc ctccagccac agccactgga aataagccga ctcgtacaat
    70021 gaagttggcg tatcggcgtt gtactccagc agcttcaccg gcgcattgcc acaccaggca
    70081 aaatccatcc gtccatacag cgaaggatcg cgggcgcgcc agctttcagc gatcacatcc
    70141 cagtacaacg gcgggatcgc cagttgcgtc aggatctctt catcttttac cgcgcgatcc
    70201 accacctcaa ggcacatctg atgcagttcc gcagtcggtt tttcgatctg ctcttcaatc
    70261 tggcgcagag taaaacggta agcccgactc tcatcccaat agatttcatt gtcgatgata
    70321 tgaaagtcga aaccgttgtc agcggcgatc tggtccagat cccgtcgcac aggaacgttg
    70381 tgtctcagca tgattagcct ccccaatgcc cacgggcgct ggaagaacga ccatagccgc
    70441 cgcgagaaac ggtagacgct tttttggtgg taaagccgcc cgaagagtaa gactcttttt
    70501 tgccagaacc ggagcgccaa gagtaatcac cagaagtgct gcgccagact gggcgcgaag
    70561 caaaagagga accgccgctg ttataaacaa acggctcatc gcgatcttta cgaataaccc
    70621 ggcttaacaa aaatccagaa acgaccggga tccaactctg ttcaacattg tcgtagtagc
    70681 aattttcgta cttagactga cagttctgct gagtcatatt cttcggaaca tcggcataaa
    70741 atgccgtttt ggcgttgttc cagccacgcg cgcaaatgtc ggcattatta ccgtcatcaa
    70801 tacaatcctg cactgtcgcg taaaacgttc cgtcgccgtc gttatcgaca tcgctactat
    70861 cgctacaacc tttcaatacg aaaaaagcgg caccgcccat tagcgccaac gttaagtatt
    70921 tttgtgcgta gcgattgcga cgcggttcaa aaggattatt cggtctacca atgcgactaa
    70981 tcgctccgtg accaatttta ctgttatttc ttgatttgcg ttttctggcc atgatcgtcc
    71041 attaccatgt catacaagct gcgttaaaaa taccgccagc cagcgcggcg gttcccataa
    71101 acattcctgc tgcggtattg tgattaataa ttttttcgct taatgcgggc atataaagtc
    71161 tgacaccagc aaaaacgagt aactgaatca ccagtgcaat cccgccccag gcgaaatagt
    71221 ctggaatact caccgcattg attgccgcac tggataaggg gataacgtaa cccaacaatg
    71281 taccgctgaa cgccagtgac gccgcggtat tattgttttt gattaactgc cattcgttgt
    71341 gcggtgtaat tttagaatag ataaacagaa aaataatcac catagccacg ccaataaaaa
    71401 aataggcgct aaaggcaaga agtgaatcca gtatatgcat tgtaaacgtc cttgtaatta
    71461 accaataata tgaagtgatg tcagcgggat atcgacaccc aacgcgcgcg aaaataacca
    71521 ctctggctcg ccgagatcgt taaaagattc ctcaccattt agcagcaaat attcgtaagt
    71581 atcttcggtt acttgccgtt ggtagcccat ggtaaaatta tggacctccc atttggcatg
    71641 gttttgattt tctacttttt ccagcatgta gacgggttcg atatttcccg gttcttcgct
    71701 gttaaaaaat cgctgccagc gtttttcctg ccagtttaag gtcattgctc ccatcgcttt
    71761 ggcgttgatc gcctcgcgcc agtgactctc tttactgata ccgtagctct cttcatagac
    71821 aaagagcttg atatcatcaa tgtcatcaat atcttcgccg ccggtggtat tgatttgcag
    71881 aaactcatcg cctgaagtgt agtagcggaa aatctgacta ccgccgccca gatcgatgtg
    71941 gctgacggcg gctaccgtaa attcctcacc cggcagcgca atcagcaatt catcttccag
    72001 caaacgaaac gctaacgtgt cgagcgtaaa gccactattg agatgaagtc ccagcggacc
    72061 acgagcgata gctggcttat tatccttgcc aaacagacgc tggaaaaaac cagacatatt
    72121 ttatccttaa aatagttgcc agccttttag cgaggctggc aaaaaaatta ctcgccctgt
    72181 tggcgttgca gtcttgctaa tacatcctgg gcgctacttt tattgctacc gccaattccg
    72241 gcttccgcca gcttttcgtc aaggtcgcga ccgtctgcga ctttctccaa ctgtgcggca
    72301 gcatccaggc gagcctgacg ttcggcctga cgcgtttgca ggcgttttaa ggattctgcc
    72361 gctgtcgaaa cgctggaaga tgcgccaacg gtagaggttg ttacagcctg ttgtgcacgc
    72421 tgcatggctt cagtggcttt aacgacttcc atttgctgct caaactgagc gatacgctgc
    72481 gctgtcgctg taactgcttt ttccacgcca tcacgggaga cttccagatt cgacaacact
    72541 tgctcttcag cggtaatgag attctcaagg cgcgcgattt cttcagcaac ttcgttaatc
    72601 aacgacggat taacgttctt gcttaacgct tccagcgcac gggcttccag actggctttg
    72661 cgctcacgta aatctttcag cttatcgtga cttaatttca cccgcgccag cagatcaacg
    72721 cgagattttc cggctttatc gagttcagct ttagcgtcac gaatatgctg ttccagcatg
    72781 cacacgccct gggtttcttc aatggattct tccgcctggg agataaacga tttccccagc
    72841 gtaaataaac tttttaaaat tcccataaat acccgtcctt gtaattagtg tgaatattct
    72901 tctgtaattt cagccagatc caatgcgtta tctaccagcg aggttatctc aagcaggata
    72961 tcttcaagag aagatttaag cgataacgcc ccgaaaacaa tgtaatactc ttcctgttgt
    73021 acgctggaga tccctaccga tgacaacggc atcatcttct gatttcttaa taagaaggta
    73081 ttaaattcat ctggattgct gatactactc accggacaaa taaaggtttc gataatcatt
    73141 tggcgggaag taaaaagaat attgatttgc aaatcgccat aatcattcat tttaataatt
    73201 aatgcgttct cgctatttgt tacatca
//
LOCUS       DBBGLQ010000018        72958 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.018, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000018 DBBGLQ010000000
VERSION     DBBGLQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.018"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            218..802
                     /gene="yadN"
                     /locus_tag="ACIYSB_001513"
     CDS             218..802
                     /gene="yadN"
                     /locus_tag="ACIYSB_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5428592.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            900..1640
                     /gene="yadV"
                     /locus_tag="ACIYSB_001514"
     CDS             900..1640
                     /gene="yadV"
                     /locus_tag="ACIYSB_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGC5428593.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVNG
                     LNGKANSAKVHFYAINDFGGAIEGNARL"
     gene            1675..4272
                     /gene="htrE"
                     /locus_tag="ACIYSB_001515"
     CDS             1675..4272
                     /gene="htrE"
                     /locus_tag="ACIYSB_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HGC5428594.1"
                     /translation="MTIEYTKNYHHLTRIATFCALLYCNTAFSAELVEYDHTFLMGQN
                     ASNIDLSRYSEGNPAIPGVYDVSVYVNDQPIINQSITFVAIEGKKNAQACITLKNLLQ
                     FHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMKN
                     YQNYVDPSLWENGINAAMLSYNLNGYHSETPGRKNESIYAAFNGGMNLGAWRLRASGN
                     YNWMTDSGSNYDFKNRYVQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRML
                     PPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSELIVTI
                     EESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNNY
                     LTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWNK
                     LFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVTV
                     SINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSTSWGSYSVSAQRSWNE
                     DGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQISSDFKGNNQLNVSSSGYSDNAR
                     VSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSISANSDNSRQVSLSTDGGFVL
                     HSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIA
                     LDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIY
                     DEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEQSKPVSCLAHYQQSPEAEKIAQSII
                     LNGIRCQIQ"
     gene            4289..4858
                     /gene="yadM"
                     /locus_tag="ACIYSB_001516"
     CDS             4289..4858
                     /gene="yadM"
                     /locus_tag="ACIYSB_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5428595.1"
                     /translation="MIKTTPHKIVILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4870..5475
                     /gene="yadL"
                     /locus_tag="ACIYSB_001517"
     CDS             4870..5475
                     /gene="yadL"
                     /locus_tag="ACIYSB_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HGC5428596.1"
                     /translation="MMTFKNLRYGLSSSVVLAASLFSVLSYAATDSIGLTVITTVEMG
                     TCTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKR
                     ATCEGTANDGAGFANGSTAADKASAVAVEVWSTVTPATGSATQFSCVTPASQEVTIST
                     AANAVVYYPMSARLVVEKNKTVNNVTAGKFSAPATFTVTYN"
     gene            5502..6098
                     /gene="yadK"
                     /locus_tag="ACIYSB_001518"
     CDS             5502..6098
                     /gene="yadK"
                     /locus_tag="ACIYSB_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGC5428597.1"
                     /translation="MHPTQRKLMKRIILFLSLLFCIACPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSGNVTPESYQPGGKEFTITVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEISSGASNVGVVIFSTQDSANTFNVLNASGGSRSVYPVMSD
                     DMNGSSWKFSTRMQKIDPALSVTSGQLMSHVLVDIYYE"
     gene            6136..7386
                     /gene="yadC"
                     /locus_tag="ACIYSB_001519"
     CDS             6136..7386
                     /gene="yadC"
                     /locus_tag="ACIYSB_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial tip-adhesin YadC"
                     /protein_id="HGC5428598.1"
                     /translation="MGVKMKTIFRYILFLALYSCCNTVSAYTSFIVGNNAGVDNYRGP
                     STAAQMTFNYTSTASNLVFYKPTQLGPTGVKMYWSYLDTGTGGGILYCNTSGRANPGP
                     ITIENAMVYSGKDYGGHKLFNTSVPGLYYTMLISRVWSAYDTITDIQSPGIYIGDPSN
                     QEFFFSVTDSDLQTKGCNKADDYDKFWAIGGIVHNITVEFYTDTNFDPTLNQQVQLSS
                     SSNYLYSFKAYSPGTKVVDHSNHIYVNFTLNNVKLTLPTCFTSILTGPSVNGSTVRMG
                     EYSSGTIKNGASPVPFDISLQNCIHVRNIETKLVTGKVGTQNTQLLGNTLTGSTAAKG
                     VGVLIEGLATSKNPLMTLKPNDTNSVYIDYETEDDTSDGVYPNQGNGTSQPLHFQATL
                     KQDGNIAIEPGEFKATSTFQVTYP"
     gene            7500..8294
                     /gene="panB"
                     /locus_tag="ACIYSB_001520"
     CDS             7500..8294
                     /gene="panB"
                     /locus_tag="ACIYSB_001520"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGC5428599.1"
                     /translation="MKPTTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8306..9157
                     /gene="panC"
                     /locus_tag="ACIYSB_001521"
     CDS             8306..9157
                     /gene="panC"
                     /locus_tag="ACIYSB_001521"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGC5428600.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9231..10133)
                     /gene="rpnC"
                     /locus_tag="ACIYSB_001522"
     CDS             complement(9231..10133)
                     /gene="rpnC"
                     /locus_tag="ACIYSB_001522"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGC5428601.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEVSQEFAQRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10407..10787
                     /gene="panD"
                     /locus_tag="ACIYSB_001523"
     CDS             10407..10787
                     /gene="panD"
                     /locus_tag="ACIYSB_001523"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGC5428602.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10791..12020)
                     /gene="yadE"
                     /locus_tag="ACIYSB_001524"
     CDS             complement(10791..12020)
                     /gene="yadE"
                     /locus_tag="ACIYSB_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGC5428603.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLSVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDNAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12084..12524)
                     /gene="yadI"
                     /locus_tag="ACIYSB_001525"
     CDS             complement(12084..12524)
                     /gene="yadI"
                     /locus_tag="ACIYSB_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGC5428604.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12629..13399)
                     /gene="yadH"
                     /locus_tag="ACIYSB_001526"
     CDS             complement(12629..13399)
                     /gene="yadH"
                     /locus_tag="ACIYSB_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5428605.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13396..14322)
                     /gene="yadG"
                     /locus_tag="ACIYSB_001527"
     CDS             complement(13396..14322)
                     /gene="yadG"
                     /locus_tag="ACIYSB_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5428606.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14431..15093
                     /gene="can"
                     /locus_tag="ACIYSB_001528"
     CDS             14431..15093
                     /gene="can"
                     /locus_tag="ACIYSB_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGC5428607.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15134..15670)
                     /gene="hpt"
                     /locus_tag="ACIYSB_001529"
     CDS             complement(15134..15670)
                     /gene="hpt"
                     /locus_tag="ACIYSB_001529"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGC5428608.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15876..18266
                     /gene="gcd"
                     /locus_tag="ACIYSB_001530"
     CDS             15876..18266
                     /gene="gcd"
                     /locus_tag="ACIYSB_001530"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGC5428609.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            18305..18403
                     /locus_tag="ACIYSB_001531"
                     /pseudo
     CDS             18305..18403
                     /locus_tag="ACIYSB_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18468..20018)
                     /gene="cueO"
                     /locus_tag="ACIYSB_001532"
     CDS             complement(18468..20018)
                     /gene="cueO"
                     /locus_tag="ACIYSB_001532"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGC5428610.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20184..20531
                     /gene="yacC"
                     /locus_tag="ACIYSB_001533"
     CDS             20184..20531
                     /gene="yacC"
                     /locus_tag="ACIYSB_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGC5428611.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20637..21503
                     /gene="speE"
                     /locus_tag="ACIYSB_001534"
     CDS             20637..21503
                     /gene="speE"
                     /locus_tag="ACIYSB_001534"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGC5428612.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21519..22313
                     /gene="speD"
                     /locus_tag="ACIYSB_001535"
     CDS             21519..22313
                     /gene="speD"
                     /locus_tag="ACIYSB_001535"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGC5428613.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22351..22713)
                     /gene="yacL"
                     /locus_tag="ACIYSB_001536"
     CDS             complement(22351..22713)
                     /gene="yacL"
                     /locus_tag="ACIYSB_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGC5428614.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22889..25486)
                     /gene="acnB"
                     /locus_tag="ACIYSB_001537"
     CDS             complement(22889..25486)
                     /gene="acnB"
                     /locus_tag="ACIYSB_001537"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGC5428615.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNVREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25841..27694
                     /gene="yacH"
                     /locus_tag="ACIYSB_001538"
     CDS             25841..27694
                     /gene="yacH"
                     /locus_tag="ACIYSB_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGC5428616.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTFPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDNDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAANQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFHDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPKIQQRREAARERIQSASPEQRQ
                     AVREKMQTNPQNQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVCERLSERGARRLER"
     gene            complement(27765..29189)
                     /gene="lpdA"
                     /locus_tag="ACIYSB_001539"
     CDS             complement(27765..29189)
                     /gene="lpdA"
                     /locus_tag="ACIYSB_001539"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGC5428617.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29514..31406)
                     /gene="aceF"
                     /locus_tag="ACIYSB_001540"
     CDS             complement(29514..31406)
                     /gene="aceF"
                     /locus_tag="ACIYSB_001540"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGC5428618.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31421..34084)
                     /gene="aceE"
                     /locus_tag="ACIYSB_001541"
     CDS             complement(31421..34084)
                     /gene="aceE"
                     /locus_tag="ACIYSB_001541"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGC5428619.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34245..35009)
                     /gene="pdhR"
                     /locus_tag="ACIYSB_001542"
     CDS             complement(34245..35009)
                     /gene="pdhR"
                     /locus_tag="ACIYSB_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGC5428620.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35550..36923
                     /gene="aroP"
                     /locus_tag="ACIYSB_001543"
     CDS             35550..36923
                     /gene="aroP"
                     /locus_tag="ACIYSB_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGC5428621.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36966..37820)
                     /gene="ampE"
                     /locus_tag="ACIYSB_001544"
     CDS             complement(36966..37820)
                     /gene="ampE"
                     /locus_tag="ACIYSB_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGC5428622.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37817..38368)
                     /gene="ampD"
                     /locus_tag="ACIYSB_001545"
     CDS             complement(37817..38368)
                     /gene="ampD"
                     /locus_tag="ACIYSB_001545"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGC5428623.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38456..39349
                     /gene="nadC"
                     /locus_tag="ACIYSB_001546"
     CDS             38456..39349
                     /gene="nadC"
                     /locus_tag="ACIYSB_001546"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGC5428624.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39552..39992
                     /gene="ppdD"
                     /locus_tag="ACIYSB_001547"
     CDS             39552..39992
                     /gene="ppdD"
                     /locus_tag="ACIYSB_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGC5428625.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            40002..41387
                     /gene="gspE"
                     /locus_tag="ACIYSB_001548"
     CDS             40002..41387
                     /gene="gspE"
                     /locus_tag="ACIYSB_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGC5428626.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41377..42579
                     /gene="hofC"
                     /locus_tag="ACIYSB_001549"
     CDS             41377..42579
                     /gene="hofC"
                     /locus_tag="ACIYSB_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGC5428627.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRENTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42614..43657)
                     /gene="guaC"
                     /locus_tag="ACIYSB_001550"
     CDS             complement(42614..43657)
                     /gene="guaC"
                     /locus_tag="ACIYSB_001550"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGC5428628.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43813..43857)
                     /locus_tag="ACIYSB_001551"
     CDS             complement(43813..43857)
                     /locus_tag="ACIYSB_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428629.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43882..44502
                     /gene="coaE"
                     /locus_tag="ACIYSB_001552"
     CDS             43882..44502
                     /gene="coaE"
                     /locus_tag="ACIYSB_001552"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGC5428630.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44502..45245
                     /gene="zapD"
                     /locus_tag="ACIYSB_001553"
     CDS             44502..45245
                     /gene="zapD"
                     /locus_tag="ACIYSB_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGC5428631.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            45255..45452
                     /gene="yacG"
                     /locus_tag="ACIYSB_001554"
     CDS             45255..45452
                     /gene="yacG"
                     /locus_tag="ACIYSB_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGC5428632.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(45668..46057)
                     /gene="mutT"
                     /locus_tag="ACIYSB_001555"
     CDS             complement(45668..46057)
                     /gene="mutT"
                     /locus_tag="ACIYSB_001555"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGC5428633.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(46117..48822)
                     /gene="secA"
                     /locus_tag="ACIYSB_001556"
     CDS             complement(46117..48822)
                     /gene="secA"
                     /locus_tag="ACIYSB_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGC5428634.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48884..49396)
                     /gene="secM"
                     /locus_tag="ACIYSB_001557"
     CDS             complement(48884..49396)
                     /gene="secM"
                     /locus_tag="ACIYSB_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGC5428635.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(49627..50544)
                     /gene="lpxC"
                     /locus_tag="ACIYSB_001558"
     CDS             complement(49627..50544)
                     /gene="lpxC"
                     /locus_tag="ACIYSB_001558"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGC5428636.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(50645..51796)
                     /gene="ftsZ"
                     /locus_tag="ACIYSB_001559"
     CDS             complement(50645..51796)
                     /gene="ftsZ"
                     /locus_tag="ACIYSB_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGC5428637.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(51857..53119)
                     /gene="ftsA"
                     /locus_tag="ACIYSB_001560"
     CDS             complement(51857..53119)
                     /gene="ftsA"
                     /locus_tag="ACIYSB_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGC5428638.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(53116..53946)
                     /gene="ftsQ"
                     /locus_tag="ACIYSB_001561"
     CDS             complement(53116..53946)
                     /gene="ftsQ"
                     /locus_tag="ACIYSB_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGC5428639.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(53948..54868)
                     /gene="ddlB"
                     /locus_tag="ACIYSB_001562"
     CDS             complement(53948..54868)
                     /gene="ddlB"
                     /locus_tag="ACIYSB_001562"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGC5428640.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(54861..56336)
                     /gene="murC"
                     /locus_tag="ACIYSB_001563"
     CDS             complement(54861..56336)
                     /gene="murC"
                     /locus_tag="ACIYSB_001563"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGC5428641.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(56390..57457)
                     /gene="murG"
                     /locus_tag="ACIYSB_001564"
     CDS             complement(56390..57457)
                     /gene="murG"
                     /locus_tag="ACIYSB_001564"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGC5428642.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRVARA"
     gene            complement(57454..58698)
                     /gene="ftsW"
                     /locus_tag="ACIYSB_001565"
     CDS             complement(57454..58698)
                     /gene="ftsW"
                     /locus_tag="ACIYSB_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGC5428643.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(58698..60014)
                     /gene="murD"
                     /locus_tag="ACIYSB_001566"
     CDS             complement(58698..60014)
                     /gene="murD"
                     /locus_tag="ACIYSB_001566"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGC5428644.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(60017..61099)
                     /gene="mraY"
                     /locus_tag="ACIYSB_001567"
     CDS             complement(60017..61099)
                     /gene="mraY"
                     /locus_tag="ACIYSB_001567"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGC5428645.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(61093..62451)
                     /gene="murF"
                     /locus_tag="ACIYSB_001568"
     CDS             complement(61093..62451)
                     /gene="murF"
                     /locus_tag="ACIYSB_001568"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGC5428646.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVSGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKLLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(62448..63935)
                     /gene="murE"
                     /locus_tag="ACIYSB_001569"
     CDS             complement(62448..63935)
                     /gene="murE"
                     /locus_tag="ACIYSB_001569"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGC5428647.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(63922..65688)
                     /gene="ftsI"
                     /locus_tag="ACIYSB_001570"
     CDS             complement(63922..65688)
                     /gene="ftsI"
                     /locus_tag="ACIYSB_001570"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGC5428648.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(65704..66069)
                     /gene="ftsL"
                     /locus_tag="ACIYSB_001571"
     CDS             complement(65704..66069)
                     /gene="ftsL"
                     /locus_tag="ACIYSB_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGC5428649.1"
                     /translation="MISRVTDALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(66066..67007)
                     /gene="rsmH"
                     /locus_tag="ACIYSB_001572"
     CDS             complement(66066..67007)
                     /gene="rsmH"
                     /locus_tag="ACIYSB_001572"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGC5428650.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(67009..67467)
                     /gene="mraZ"
                     /locus_tag="ACIYSB_001573"
     CDS             complement(67009..67467)
                     /gene="mraZ"
                     /locus_tag="ACIYSB_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGC5428651.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIYHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(68069..69073)
                     /gene="cra"
                     /locus_tag="ACIYSB_001574"
     CDS             complement(68069..69073)
                     /gene="cra"
                     /locus_tag="ACIYSB_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGC5428652.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(69155..69241)
                     /locus_tag="ACIYSB_001575"
     CDS             complement(69155..69241)
                     /locus_tag="ACIYSB_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGC5428653.1"
                     /translation="MRNLQPNMSRWAFFAKSVGAWNKSSCRS"
     gene            complement(69253..69744)
                     /gene="ilvN"
                     /locus_tag="ACIYSB_001576"
     CDS             complement(69253..69744)
                     /gene="ilvN"
                     /locus_tag="ACIYSB_001576"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGC5428654.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSGKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(69747..71471)
                     /gene="ilvI"
                     /locus_tag="ACIYSB_001577"
     CDS             complement(69747..71471)
                     /gene="ilvI"
                     /locus_tag="ACIYSB_001577"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGC5428655.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            71590..71634
                     /locus_tag="ACIYSB_001578"
     CDS             71590..71634
                     /locus_tag="ACIYSB_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428656.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(71789..72733)
                     /gene="leuO"
                     /locus_tag="ACIYSB_001579"
     CDS             complement(71789..72733)
                     /gene="leuO"
                     /locus_tag="ACIYSB_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGC5428657.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        17992 a        19647 c        17632 g        17687 t
ORIGIN      
        1 tgtgaactta tttttactta attttgaatt aagtaaattt aatttgttca aaagcttcta
       61 aagtgcacaa taggcgacct aatgaacaat ggttgtaaat ttgggtaaca aatatcattg
      121 ctcattaata tctaacattt gtttctccta tttgtgtcat gaatagtcat tcttcaaaca
      181 tgtagtctga ctaatggaat tttattaaag gatttttatg tctaaaaaat taggttttgc
      241 cctgtctggt cttatgctgg cgatggttgc aggtactgct tctgctgata tggacggcgg
      301 tcagttaaat atcagtggtc tggttgttga taacacctgt gaaacccgcg ttgacggcgg
      361 taacaaggat ggtctgatcc tgctgcaaac cgcaaccgtt ggtgaaatcg atgctggcgt
      421 actgaatgac accgttggcg ctaaagctaa acctttcagc atcaccgttg actgcagcaa
      481 agcaaatccg aatccaggta gcacggctaa aatgaccttc ggttctgttt tctttggtaa
      541 cagcaaaggg actttgaaca acgacatgtc tattaacaac ccatctgacg gcgttaatat
      601 cgctctacac aatattgatg gttccactat caaacaggtt caaattaaca accctggcga
      661 tgtgtatact aaagccctgg atgcaacgac aaaatctgct gtttatgatt ttaaagcgtc
      721 ttacgttcgt gccgtcgcag accaaacagc aactgctggt tatgtaaaaa ctaacactgc
      781 atacaccatt acttatcagt aagtattcac cagttaaatt gattgaatga atatacaggg
      841 aataataatt tctattttat attattccct gttttaatta actctatcag ggatggttta
      901 tgttttttaa caccaaacat acaacagctt tatgcttcgt aacctgtatg gcttttagtt
      961 catcgtctat tgcggacatt gtcatttcgg gtactcgcgt aatatataaa agcgatcaaa
     1021 aaagtgtcaa cgtacgtctg gaaaataaag ggaataaccc gttgcttgtc cagagttggt
     1081 tagatactgg cgatgacaac gctgagcctg gcagtattac agtccctttt actgctacgc
     1141 cgccagtatc gcgtattgat gccaaacgtg ggcaaacaat caaattaatg tacacagcca
     1201 gcacctcact gcctaaagac agagagagcg tgttctggtt taacgtactg gaagttccac
     1261 caaaaccaga tgcagaaaag gtcgcgaatc aaagcctgct gcaactggca tttcgcacac
     1321 gtataaaact tttctatcgc ccggatggat tgaagggaaa tccctctgaa gccccgttag
     1381 ccctgaagtg gttctggtca ggttcagaag gtaaggcgtc attacgagtg accaatccaa
     1441 ccccttacta cgtctctttt agcagtggtg atttagaagc tagcggtaaa cgctatccga
     1501 ttgatgtgaa aatgattgca ccatttagtg atgaggtcat gaaagtcaat ggccttaatg
     1561 gcaaagcgaa ttctgcaaaa gtgcattttt acgccattaa tgactttggt ggcgcaattg
     1621 aaggtaatgc caggctgtaa tcaggcaagg atataattcc gcaggaagca tagcgtgact
     1681 atagaatata ctaaaaatta tcatcatctg acccgtatcg ccacgttttg cgcgctgctg
     1741 tattgcaata ctgctttcag tgctgaactc gttgaatatg accatacctt cctgatgggg
     1801 cagaatgcat ctaatattga tctcagccgg tacagtgaag gtaaccccgc tataccgggt
     1861 gtttacgacg tcagtgttta tgtaaacgac caaccaatca ttaaccaaag tattacattt
     1921 gtcgcaattg aaggaaaaaa gaacgcccag gcttgtatca cattaaagaa tttattgcag
     1981 tttcatatta attctcccga tataaataac gaaaaagccg ttctgcttgc cagggatgaa
     2041 acgctcggca attgcctcaa tttgacggaa attatccctc aggcttctgt tcgttatgac
     2101 gttaacgatc aacgtctgga tatagacgtt cctcaagcct gggtaatgaa aaattaccaa
     2161 aactatgttg atccatcgtt atgggaaaac ggcattaatg cggccatgtt gtcatacaac
     2221 ctcaacggat atcatagtga aacccctggt cgaaaaaatg aaagcattta tgctgcattt
     2281 aacggtggga tgaatttagg tgcatggcga ctgcgtgcct cgggcaacta caactggatg
     2341 accgattctg gcagtaatta tgattttaag aatcggtatg ttcagcgtga tatcgcctcg
     2401 ctgcgttctc aactcattct tggtgagtct tatacgacgg gcgaaacctt tgattccgtc
     2461 agtatccgag gcattcgttt atacagtgac agccgcatgt tgcctccgac tttagccagc
     2521 tttgcgccta tcattcatgg cgttgccaat accaacgcca aagtcactat tacgcaaggt
     2581 ggctataaga tttatgaaac gacggtgccg ccaggcgctt tcgtcattga tgatctgagt
     2641 ccgtcagggt acggcagcga gcttattgtt accatcgaag aatccgatgg ctcaaagcgg
     2701 acattctcgc aacctttctc atccgttgtt caaatgttac gccctggcgt tggacgttgg
     2761 gatattagcg gcggtcaggt cttaaaagat gatattcagg atgagcctaa tttatttcaa
     2821 gcaagctact actacggcct gaataactat ctgacgggtt ataccggtat tcagataacc
     2881 gataataact ataccgctgg tttgttaggt cttggtctga atacttcagt tggtgcattt
     2941 tctttcgatg tgactcattc caatgttcgt atcccggatg ataaaacata ccaggggcaa
     3001 agttatcgtg tttcctggaa caagttattc gaagaaacaa gtacttcact gaatatcgcg
     3061 gcctatcgct attcgacaca gaattacctt ggtcttaatg atgcactaac tctaattgat
     3121 gaagtgaaac atcccgaaca agatcttgaa ccgaaatcca tgcgtaatta ctcacgcatg
     3181 aaaaatcagg ttacggtcag tattaaccaa ccgttgaaat ttgagaaaaa agattacggt
     3241 tcattttatc tttccggaag ttggtccgat tactgggctt ccggacaaaa tcgtagcaat
     3301 tactctattg gctacagtaa cagtacatcc tggggcagct acagtgtcag tgcccagcgt
     3361 tcatggaatg aagacggcga cactgacgat agcgtttatc ttagtttcac cattccaatt
     3421 gaaaaattac ttggcactga acaacgtact tcaggtttcc agagtattga tactcaaata
     3481 agcagtgact ttaagggtaa taaccaactc aacgttagca gcagtggcta tagcgataac
     3541 gctcgcgtca gttatagcgt gaatactggc tatacgatga ataaagccag caaagatttg
     3601 agttatgttg ggggttatgc cagctatgag tcaccatggg gaacgctggc aggttcaatt
     3661 tctgcaaata gcgataacag ccgtcaagtt tctctcagca ccgacggtgg ttttgtattg
     3721 catagcggtg gactgacttt cagtaatgat agttttagcg actccgatac actggcggta
     3781 gttcaggctc caggtgctca aggagcgcga ataaattatg gcaacagtac tatcgatcga
     3841 tggggttatg gtgtcaccag cgctctttct ccttatcatg aaaaccgtat cgcgctggat
     3901 atcaacgatc ttgagaacga tgttgaatta aaaagtacca gtgcagtagc tgtaccgcgt
     3961 cagggttcag tcgtctttgc tgattttgaa accgtgcaag ggcaatcagc cattatgaac
     4021 atcacacgaa gtgatggtaa aaatattcca tttgctgcag atatttatga tgagcaaggc
     4081 aatgtcattg gtaatgttgg acagggtgga caagcatttg ttcgtggtat tgagcagcag
     4141 ggaaatatca gcattaaatg gctcgaacaa agtaaacccg taagttgtct tgcgcattat
     4201 caacaaagcc cagaagcaga aaaaatagca caatctatta ttctgaatgg aattcggtgt
     4261 cagattcagt aactacaagg gacgtcaaat gataaaaaca acgccacata aaatagtgat
     4321 actgatggga atattattat caccctcagt atttgcaacg gatattaatg tagagtttac
     4381 agccactgtc aaagcgacaa cctgtaacat cacacttact ggtaataacg tcacgaatga
     4441 tggcaataat aactacacat tgagaatccc taagatgggt ctggataaga tcgcgaataa
     4501 aacgacagaa tctcaggctg attttaaact ggttgccagt gggtgcagca gtggcatcag
     4561 ttggattgat accactctga ccggaaatgc atcatcaagc tcacctaagc ttattatacc
     4621 gcagtctggt gattcatctt cgacgacaag taatatcggt atgggtttca aaaaacggac
     4681 tactgatgat gccactttcc ttaaacctaa cagtgcggaa aagatacgct ggagcacaga
     4741 cgagatgcag cccgataagg gtcttgaaat gaccgttgcg ctacgtgaaa cagatgcagg
     4801 gcaaggcgta ccggggaatt ttcgtgcact ggcgacgttt aatttcatct atcaataaga
     4861 aggataatta tgatgacatt taaaaattta cgttatggat tatccagcag tgtcgttttg
     4921 gctgcctcat tgttcagcgt actctcttat gcggcaacag acagtattgg actgaccgtt
     4981 attactactg tagaaatggg tacttgtacc gctacattag taaatgactc tgatcaggac
     5041 atttctgttg ttgattttgg tgatgtatat atttctgaaa tcaatgccaa gaccaaagta
     5101 aaaacattca aactcaaatt caaagactgt gcgggtatcc ccaataaaaa agcgcaaata
     5161 aaattaacca agcgagccac atgcgaggga actgctaatg acggtgcggg gtttgcaaat
     5221 ggttccacag ccgcagataa agcaagtgct gtcgccgttg aagtctggag cactgtaact
     5281 ccggcaacag ggagtgcaac acaatttagc tgtgtaacac cagcatcaca agaggtaaca
     5341 atctccactg cagccaatgc ggtcgtttat tatccgatga gtgcacgcct ggtcgtggaa
     5401 aaaaataaaa ccgtaaacaa tgtcactgcg ggtaagtttt ctgcaccagc cacatttaca
     5461 gtaacctata actaacagac ttacagattc aggaactata tatgcatccc actcaacgta
     5521 agctgatgaa gagaataatt ctgtttctgt cattactgtt ttgcatcgcc tgtccagcca
     5581 ttgctggaca ggatattgac cttgttgcca atgtaaaaaa cagcacctgc aaaagcggaa
     5641 tcagtaacca gggtaatatt gatcttggcg tcgttggggt gggatatttt tcaggtaatg
     5701 ttactcctga aagttatcaa ccaggtggaa aagagttcac tatcactgta tccgactgtg
     5761 cattacaggg aactggcgat gtgctaaatc agttacatat tgattttaga gcccttagcg
     5821 gtgtcatggc tgctggctct aggcaaatat ttgctaatga aatttcgtca ggagcaagta
     5881 atgtaggagt agttatattt tctactcagg attcggcgaa tacattcaat gttcttaatg
     5941 cttcaggcgg atctcgttcc gtttatccag taatgtcgga tgacatgaat ggttcatcct
     6001 ggaaatttag cacccgaatg caaaaaatcg atcctgcatt gagtgttaca tctggtcaac
     6061 ttatgagcca tgtgttagtg gatatttact acgaataaca aacaaaaaca gcgcaaacca
     6121 taacaataaa gaaacattgg agtaaaaatg aagactatct tcaggtacat tcttttttta
     6181 gcactgtatt cttgttgtaa tacagtcagt gcatatacaa gttttattgt gggaaataat
     6241 gcaggagttg ataactatcg aggcccctcc actgccgcac agatgacctt taattacaca
     6301 tcaacagcaa gcaacttggt tttttataaa cccacgcagc tcggcccgac tggggtaaaa
     6361 atgtactggt catacctgga tacaggtacc ggtggtggta ttctttactg caatacatct
     6421 ggcagagcga atcctggtcc aataactatt gaaaatgcca tggtctattc aggtaaagat
     6481 tatggcggac ataaactatt taatacatct gttcctggtc tgtattacac catgttaata
     6541 tcaagggtct ggtctgcata cgatacaata actgacattc aatcgccagg aatctatatc
     6601 ggagatcctt ccaaccaaga atttttcttt tccgtcacag acagcgatct acaaactaag
     6661 ggttgcaaca aagcagacga ctacgataag ttttgggcta ttggtggtat agtacacaac
     6721 ataactgttg aattttatac agatactaat ttcgatccta cacttaatca gcaagtccag
     6781 ttatccagtt catcaaatta cctttattcg tttaaggcct acagtcctgg tacaaaagtt
     6841 gtagatcaca gcaaccacat ttatgtcaat ttcacactga ataatgtcaa attaacctta
     6901 ccaacatgtt ttacctccat acttaccggg ccatcagtca atggttcaac ggttagaatg
     6961 ggagaatata gctctgggac aattaaaaat ggcgcttcac ctgttccctt tgatatctcg
     7021 cttcagaatt gtattcatgt gcgtaatatt gaaacaaaac tcgtcactgg aaaagtaggt
     7081 actcaaaaca cacaactgct tggtaatacg cttactggaa gcactgccgc caaaggcgtt
     7141 ggcgtactca ttgaaggttt agcaaccagt aaaaatccgc taatgacatt aaaacctaat
     7201 gatacgaatt ctgtttatat agattatgaa accgaagatg acacctccga tggggtttac
     7261 cctaatcaag gtaacggcac atcacagcct cttcatttcc aggcaacatt aaagcaagac
     7321 gggaatattg ctatcgaacc cggcgaattt aaagccacca gtactttcca ggtaacctat
     7381 ccctgataac gtagcagcac tgtcaccttc tggcagtgct gctacggata aatcattgcc
     7441 cccaccaatg tgactgataa aatgccagac agcacaacat caatttatca ggatacgtta
     7501 tgaaaccgac caccatctcc ttactgcaga agtacaaaca ggaaaaaaaa cgtttcgcga
     7561 ccatcaccgc ttatgactat agcttcgcca aactctttgc tgatgaaggg cttaacgtca
     7621 tgctggtggg cgattcgctg ggcatgacgg ttcaggggca cgactccacc ctgccagtta
     7681 ccgttgccga tatcgcctac cacactgccg ccgtacgtcg cggcgcacca aactgcctgc
     7741 tgctggctga cctgccgttt atggcgtatg ccacgccgga acaagccttc gaaaacgccg
     7801 caacggttat gcgtgccggt gctaacatgg tcaaaattga aggcggtgag tggctggtag
     7861 aaaccgtaca aatgctgacc gaacgtgccg ttcctgtatg tggtcactta ggtttaacac
     7921 cacagtcagt gaatattttc ggtggctaca aagttcaggg gcgcggcgat gaagcgggcg
     7981 atcaactgct cagcgatgca ttagccttag aagctgctgg ggcacagctg ctggtgctgg
     8041 aatgcgtgcc ggttgaactg gcaaaacgta ttaccgaagc actggcgatc ccggttattg
     8101 gcattggcgc aggcaacgtc actgacgggc agatcctcgt gatgcacgac gcctttggta
     8161 ttaccggcgg tcacattcct aaattcgcta aaaatttcct cgccgaaacg ggcgacatcc
     8221 gcgcggctgt gcggcagtat atggctgaag tggagtccgg cgtttatccg ggcgaagaac
     8281 acagtttcca ttaaggagtc acgttgtgtt aattatcgaa accctgccgc tgctgcgtca
     8341 gcaaattcgc cgcctgcgta tggaaggcaa gcgcgtggcg ctggtgccta ccatgggtaa
     8401 cctgcacgat ggccatatga agctggtcga cgaagccaaa gcccgcgccg atgtggtcgt
     8461 cgtcagtatt ttcgttaacc cgatgcagtt cgaccgcccg gaagatctgg ctcgttatcc
     8521 acggaccttg caggaggact gcgagaagct aaacaaacgt aaagtggatt tagttttcgc
     8581 cccttcggta aaagagatct acccgaacgg tactgaaacc cacacttacg ttgacgttcc
     8641 tggcctttcg accatgctgg aaggtgccag ccgtccggga cattttcgcg gcgtttcgac
     8701 tattgtcagc aagctgttca acctggtcca gccggacatc gcctgcttcg gtgaaaaaga
     8761 ttttcagcaa ctggcgctga tccgcaaaat ggttgccgat atgggcttcg atattgagat
     8821 tgtcggtgtg ccaattatgc gcgccaaaga cggtctggcg ctaagttccc gtaacggtta
     8881 tctgacggcg gaacaacgca aaattgcgcc tggtctgtac aaagttttaa gttcgattgc
     8941 tgacaaattg caggctgggg aacgggatct cgatgaaatt attaccattg cggggcaaga
     9001 actgaatgaa aaaggcttcc gcgccgatga tattcagatt cgcgatgccg acacattgct
     9061 ggaagtttct gaaaccagca aacgggcagt aattctggta gccgcctggc ttggcgatgc
     9121 tcgcctgatc gacaacaaaa tggtcgagct ggcgtaatac ttaactggcg ctacggctga
     9181 tggcgccagt tattaattta ccccacgtca tctgtcataa cacaggtaac ttaaataagg
     9241 ttaattacct tatcaatctc agcaagaggt aaatttgcca tctctgcaac gtcttcccga
     9301 gacattcctt tactcagaag acgctgggcg aattcctgac ttacttcctg tcttccctgc
     9361 tgaatcccct tctcaatccc tttctcttca aaccactgcg ccagcgtcat catagactcc
     9421 cctcccgttt ccctgtctct caacacaccg taaaacaaat ccgcttgttc agtatgaccg
     9481 cgttgcagca tatagttttg catggcaact aactgacttc cgctagtgta cccttcgtcg
     9541 atcagcgtga ccagttgctc aagcaataac attaagtcgc gctggcgaat atgtttttgc
     9601 agtagttcga gaatcgcaat ccgccgatgt tgcatgattt cgtcatccgg tgtgatggtg
     9661 atatccacca gcgggaaagg actgttatag acgcgtcgcg ccagctccgg cgagtaaaac
     9721 atatcaaacc agcacattga tagcggataa ggtgtggcct cgccctgata aaacagtatc
     9781 ggcaccacca gcggcagctt atcgtggtca gcctccagat gccggtgcat ggcggctata
     9841 gaataacgca tcatgcgaaa ggccattttc ttatccggct tgctttggtg ttcaatcaca
     9901 acatgcagat aaccgggatt gccctgcatt tgcacggaat agagcacgtc cgtgctgtgt
     9961 cctttcaggc tctcttcaat gaaactcccc gactctaaat gaagcgtgtt gaggtcacaa
    10021 agttcgcgta attccactgg caaatgtatc tccagaaagt cgcgagccgt ctccgcatgc
    10081 attaaaaatt gtttaaatac cgcgtcatgc ggtgtggtac tcggtgcatc catcttccct
    10141 gtcttccgtg tggctttgcg tgaaggcagt atccagtcaa ggcgcgctat cgtcagccac
    10201 ctctttattg tttaccgagc agcgttcaga gaaatatcaa ttcgttacag gcgatacatg
    10261 gcacgcttcg gcgcggtacg caaactgctg acgcaaatgc cgtcagatga gtagacacta
    10321 aacaaaaatc gggcaatact gcgtgagaat ttcctcaaag caggcatcgc ctgcttcgtt
    10381 aacgacaggg tagaaaggta gaagttatga ttcgcacgat gctgcagggc aaactccacc
    10441 gcgtgaaagt gactcatgcg gacctgcact atgaaggttc ttgcgccatt gaccaggatt
    10501 ttcttgacgc agccggtatt ctcgaaaacg aagccattga tatctggaat gtcaccaacg
    10561 gcaagcgttt ctccacttat gccatcgcgg cagaacgcgg ttcgagaatt atttctgtta
    10621 acggtgcggc ggcccactgc gccagtgtcg gcgatattgt catcatcgcc agcttcgtta
    10681 ccatgccaga tgaagaagct cgcacctggc gacccaacgt cgcctatttt gaaggcgaca
    10741 atgaaatgaa acgtaccgcg aaagcgattc cggtacaggt tgcttgattg ttatccctgc
    10801 ggctggttac tcaccagccg cgacatcgtc tccagcgaat ccgttcttaa gatataaagt
    10861 cgttttagta acaacggatt atcccccggt tttactttgc ctttcatggt tgtcaccgcc
    10921 aggtgaaatc cggcatcgtt tgctgccttc acggcgttgt cattaaatcc gccaaacgga
    10981 tacgaaagat accagacatg cggattaaat tgcgccagag cgcggcgtga acgtgcaaaa
    11041 tcaaacagaa tattgtgctc actacggctc agtaatatgg gtcggcgata accatctacc
    11101 cgatgcaaaa aatgggtatg tgactggaaa tcaaatacat cgcgaatttc gttaagctca
    11161 gaaacgctca taaattgcag cgattttggg ttccacttct gcgggtgacg tttgatgcgt
    11221 gaggtaacaa taaacgccgt cgccttcatg ccatattgtt tcaacacagg atacgcatag
    11281 cggctcaccg acttgaggcc atcatcaaag gtaatcacca ccgctcgcgc agggagattg
    11341 atcttattct tcacgtagcc ttccagctgc accatgctca gtgtcgcgta tcccctgtca
    11401 cgcagccagg ccatctggtt attgaaagcg cgtaccgatg tggtcgtcga agtatggcga
    11461 aaacgggtgt tttcttcgtc gcgcagaata tggtgatagg ttagcaccga caggccatta
    11521 tcgggttggg catccagtgc gctgatatag gccagtcgat cgccaatacg gatctgatac
    11581 caggtttgat ttaacctgtc tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc
    11641 agtaccccaa atggcgcact tcccgcactc ggcgcgttat agaccggcgt atctttccag
    11701 gtaactaagt tctgattact cagcggcttg ttgaggtcgc ccaaaccgtc ttcaactttt
    11761 tgtcgcccct gaaccggctc gagatgacct ttatcgataa aacctttgcc aaagccaaaa
    11821 ttaaatgcgt aataacttgc ggcagtgggc tccacggcaa tgatttgtcc ggcccgaata
    11881 ttccccacgg tcaccatctt gtcaccaatt tgcgcccaga ccgcagcatt ttcgatggtt
    11941 tgcatataac gggcaggtaa cgcggcactg acacttgcgg taaacagcat cagcaggaga
    12001 ataacagctt gtttgtacat gaaaaaataa ccgagggtga aacaacgctt ttattgtagc
    12061 aaaagcgcca tcagaatcgc gggttaatac tcatacaaat tatgtttgag gacgaaaggc
    12121 ggattttttt gttgttggtg ccaaagacta ctcacctccg gcgcacccag ttcgacaata
    12181 cgctcgcgaa actctgaact ggtcatggtt tcacggcaag ccatcatctg ttcaattaac
    12241 ggtaacgtga caccagaaat cacttcgcaa cgggagtgtt tgtggcttaa taatgaagcc
    12301 acgcgatacg gtggcgctcc ggctatatcg gttaagaaga tgacaccctc accagagtcc
    12361 gcttcatgca gagcatcgca catcatgcgg ctgagcatat tagagcttaa tccgcgccag
    12421 aaattcacgg cccggcactg aagaagtgcc ccatgttttt tctccagcgc atccagtatc
    12481 tcttgcgccc ggtcatcgtg acaggtaatt acccaaccta acatcgcctt cctccttagc
    12541 cgaaaatcag tctaccctga ccattgttga taaagggtga tagccgtcaa aactcctctc
    12601 ccccaaatcc agaggagagg aaatagcctt agctacgcaa accacgtcca cgttggatca
    12661 gcgaccaaca gatcaaataa aacgccacaa taaagaccac cagtacgcca aaggtagtga
    12721 ccagcggaac atcattgata ccgaggaagc cgtagcggaa accactgatc atataaacga
    12781 ttgggttcag gtgcgacagc ccttgccaga acggcggcaa caaagtcagt gagtaaaaga
    12841 ccccgcccaa atacgtgagt ggcgttaaca caaaggttgg caccaggctg atgtcatcga
    12901 acgttttggc aaacacaccg ttcagcaaac ccgcaaggga gaacaacacc gccgtgagca
    12961 ccagcgttaa ggcaacgaat acccacgaat gcacctgaaa tggcacaaaa aacagtgaaa
    13021 ttgccgtcac cagaatgcca acaaacagac cacgcgccac gccaccgccg acatatccgg
    13081 caataatgac gtgagtcgga accggcgcta ccagcagctc ttcaatatta cgctggaact
    13141 tggcaccaaa aaatgatgac gcaacgttgg cgtaggcatt ggtgatcacc gacatcatga
    13201 tcagccccgg tacgatgaac tgcatatagc tgaagccatg catatcgcca atacgcgaac
    13261 caatcaggtt accgaagata ataaagtaaa gggtcatggt gatgactggc ggcaccagcg
    13321 tctgcaccca gatacgcata aagcgatgga tctctttcgc ccagatgctt tttagcgcca
    13381 cccagtaaag atgcatcatg cgcgatctcc ttgtttttca ttaaccagtg aaacaaacag
    13441 ctcttccaga cggttagctt tgttacgcat acttaatacc tgaatgccct gctcacttaa
    13501 ctgcgtaaat acgctgttga tcccctgctc acgcagcact tcaacttcca gcgtcgcggt
    13561 atcgaccagt cgatactgat agccatcgag cttcggtaac gggcttttcg gtgcgagatc
    13621 gagaataaag gtttccgatt tcagcttcgc cagcagcgcc ttcatcgagg tattttccac
    13681 cagctcaccg tgttgaataa tgccgatatt gcggcacagc atttctgctt cttccaggta
    13741 gtgtgtggtg agaatgatgg tggtgccttt gtcgtttaaa tccttcaaaa agccccacat
    13801 tgagcggcga agttcaatat ccacgcctgc ggtcggttcg tcgagaatca gtagtttagg
    13861 ttcatgcatt aacgcacggg caatcattaa acggcgcttc atcccgccag ataacatacg
    13921 cgcacgttcg ttgcgttttc cccatagatc gagttgttta agatactttt cgctgcggat
    13981 gtacgcttct ttgcgctcca cgccgtagta ccctgcctga ttcaccacaa tttgctgcac
    14041 ggtttcaaac gggttgaagt taaattcctg cggcaccagt cccaactgac gtttagcgtt
    14101 cacgacatcc ttctcgagat cgtaaccaaa tacgctgacc cgcccggagg ttttatttac
    14161 cagagagctg ataataccga tagtggtcga tttcccggcc ccgttcggcc cgagaagcgc
    14221 ataaaaatca cccgcttcga cctgcaaatc tatcccacga agcgcctgaa cgccgcctgg
    14281 ataggttttt ttaagctgtt gaagttccag tgcaatggtc ataaattttt acttacctta
    14341 cgttcttaca ctttatctgt ggtttaaatc gtcccggagt tgccctatat tagccaaacg
    14401 taattatttg gttacaggtc gttaacctcc atgaaagaca tagatacact catcagcaac
    14461 aatgcactat ggtcaaaaat gctggtggaa gaggatcccg ggttttttga gaaactggca
    14521 caagcgcaaa aaccgcgctt tctatggatt ggatgttccg acagtcgcgt tcctgcagaa
    14581 cgtttaaccg gtcttgagcc gggcgaactc tttgttcacc gtaatgttgc taacctggtc
    14641 attcacactg acctgaactg cctttccgtg gttcagtatg cagtggatgt actcgaagtt
    14701 gaacacatta ttatctgtgg ccactacggt tgcggcggcg tacaagccgc agttgaaaac
    14761 ccggaactgg ggcttatcaa caactggctg ctgcatatcc gcgatatctg gttcaaacat
    14821 agctcattgc tcggcgaaat gccgcaagag cgccgtctgg ataccttgtg tgaactgaac
    14881 gtcatggaac aggtgtataa cctgggccac tccaccatta tgcaatcagc gtggaaacgc
    14941 gggcagaaag ttaccattca cggctgggcc tacggcattc acgacggctt gctgcgtgat
    15001 ctggatgtta ccgccaccaa ccgcgaaacc cttgagcaac gttaccgtca cgggatttcc
    15061 aacctcaagc tgaaacacgc caaccacaaa taaaaatgcc atgccggatg caacacatcc
    15121 ggcaacttca cacttactcg tccagcagaa tcactttgcc gatatacggc agatgacggt
    15181 aacgctgtgc gtaatcaatg ccgtaaccca ccacaaactc atccgggatc gagaaaccga
    15241 taaattctac cgggacgttc acttcacgac gggacggttt atccagcagc gtacaaatcg
    15301 ccagcgactt cggttcgcgc aggcttaaga tctcacgcac tttcgacagt gtattccccg
    15361 agtcgatgat atcttcaaca atcagcacgt ccttgccacg gatatcttca tccagatctt
    15421 tgaggatttt cacatcacgg gtggtggaca tgccgctacc gtagctggag gcggtcataa
    15481 agtcgacttc atgagatacc tgaacttcac ggcacaggtc cgccataaac ataaatgagc
    15541 cacgcagcag acccaccagc accatatcgc tgccgctgtc tttgtaacgc tcagtaatct
    15601 gacgacccag ttcggcgata cgcgctttaa tctccgcttc ggggatcatt acttctacag
    15661 tatgtttcat atctctaacc atatgattta aaaacaaatc acttcgcacg ggcaggtttt
    15721 ggcaccgcaa caaagcgcaa gacacgcagt ataccagtaa aactattcat tcgcggaatc
    15781 tgttaataaa gctcgaattg tgatgacgat cacacatgtt aaaacctata attaattgct
    15841 actaaaatat taatgaattg aaatggtgtc tctttatggc aattaacaat acaggctcgc
    15901 gacgattact cgtcacgcta acagcccttt ttgcagcgct ttgcgggctg tatctactca
    15961 ttggcggagg ctggctggtc gcgattggcg gctcctggta ctaccctatc gctggccttg
    16021 tgatgctcgg cgtcgcctgg atgctgtggc gcagtaaacg cgccgcgctt tggctatacg
    16081 cagccctgct gctcggcacc atgatttggg gcgtctggga agttggtttc gacttctggg
    16141 cgctgactcc gcgcagcgac attctggtct tcttcggcat ctggctgatc ctgccgtttg
    16201 tctggcgtcg cctggtcatt cctgccagcg gcgcagttgc cgcactggtg gtcgcactgc
    16261 tgattagcgg tggtatcctg acctgggccg gatttaacga tccgcaggag atcaacggca
    16321 ccttaagcgc cgatgccaca cctgctgaag ctatctcccc cgtagccgat caggactggc
    16381 ctgcctatgg tcgtaatcag gaaggtcaac gcttttcgcc gctgaaacaa attaacgccg
    16441 ataacgtcca taatctgaaa gaagcctggg tgttccgtac tggcgatgtg aagcagccga
    16501 acgatccggg tgaaatcacc aatgaagtga cgccgattaa agtgggcgac accctttacc
    16561 tgtgtaccgc tcaccagcgc ctgtttgcgc ttgatgccgc cagcggcaaa gagaaatggc
    16621 attacgatcc tgagctgaaa accaacgagt ctttccagca cgtaacctgc cgtggtgtct
    16681 cttatcatga agccaaagca gaaaccgctt cgccggaagt gatggcggat tgcccgcgtc
    16741 gtatcattct tccggtcaat gatggtcgac tgattgcgat taacgctgaa aacggcaaac
    16801 tgtgcgaaac cttcgccaat aaaggcgtgc tcaatctgca aagcaatatg ccagacacca
    16861 aaccgggtct gtatgaaccg acttcgccac cgattatcac cgataaaacc atcgtgatgg
    16921 ccggttcagt taccgataac ttctcaaccc gcgaaacgtc tggcgtgatc cgtggttttg
    16981 atgtcaacac cggggagctg ctgtgggctt ttgatcccgg cgcgaaagat ccgaacgcaa
    17041 tcccgtctga cgaacacacc tttaccttta actcgccaaa ctcctgggca ccagcggcct
    17101 atgacgcgaa gctggatctg gtctatctgc cgatgggcgt gaccacgccg gatatctggg
    17161 gcggtaaccg cacaccggaa caggaacgtt atgccagctc gattctggcg ctgaatgcca
    17221 ctaccgggaa actggcgtgg agctaccaga ccgttcacca cgacctgtgg gacatggatc
    17281 ttccggcaca gccgacgctg gcggacatca ccgttaatgg tcagaaagtg ccagttattt
    17341 acgctccggc gaaaaccggc aacatttttg tgctcgatcg tcgtaatggc gaactggtgg
    17401 ttccggcacc ggaaaaaccg gttccccaag gtgcagcgaa aggcgattac gtaaccccaa
    17461 ctcaaccgtt ttctgaactg agcttccgtc cgacgaaaga tttgagcggt gcggatatgt
    17521 ggggagccac catgtttgac caactggtgt gccgcgtgat gttccaccag atgcgctatg
    17581 aaggcatttt caccccgcca tctgaacagg gtacgctggt cttcccgggt aacctgggga
    17641 tgttcgaatg gggcgggatt tccgttgatc caaatcgtga agtggcgatt gccaacccaa
    17701 tggcactgcc gtttgtttcg aaactgatcc cgcgtggtcc tggcaacccg atggagcagc
    17761 cgaaagatgc caaaggcacg ggtacggaat ccggcattca gccacagtac ggtgtaccgt
    17821 atggtgtcac gctcaacccg ttcctctcac catttggtct gccatgtaaa cagccagcat
    17881 ggggttatat ctcggcgctg gatctgaaaa ctaatgaagt ggtgtggaag aaacgtattg
    17941 gtacgccgca ggacagtatg ccgttcccga tgccggttcc ggtgccgttc aatatgggta
    18001 tgccgatgct gggcgggcca atctccacgg cgggtaacgt gctgtttatc gccgctacgg
    18061 cagataacta cctgcgcgct tacaacatga gcaacggtga aaaactgtgg cagggtcgtt
    18121 taccagcggg tggtcaggct acgccaatga cctatgaagt gaatggtaag cagtatgtgg
    18181 tgatctccgc aggcggtcac ggttcatttg gtacgaagat gggcgactat attgtggctt
    18241 atgcgctgcc ggatgatgtg aagtaagact tgctcagatt gctgacaacg tgcgcgttgt
    18301 tcatgccgga tgcggcgtga acgccttatc cagcctacaa aattgtgcaa aatcaatgga
    18361 ttgcacagct agcgtaggcc tgataagcgt agcgcatcag gcagatttgc gtttgtcatc
    18421 agtttaatgc ccggagagat ccgggcatat ttccgaatac ggtcttttta taccgtaaac
    18481 cctaacatca tccccgtatc ttcatgctcc agcagatggc agtgcgccat ataagcatgt
    18541 tctttcggtg catcgtgatt aaacttcacc agcacttcgc tgacattacc ttctacctta
    18601 acggtatctt tccagcccgc gcgatgagcc gctggcggtt tgccattttc tgacaagata
    18661 cggaactgcg tgccgtggat atggaacgga tgcagcatca tgtcgcccac gccagagata
    18721 acccaacgtt cgtattgccc tttcgccgcc gcaaacatcg gcttgttcat atcaaacgcc
    18781 tgaccgttga ttttgttggc atggtggaaa tcgaacttcc cgccgtggtt catatgattc
    18841 atattgccgt gccccatatg gcccatcatc tggctgtgat ccatcccggc catcgcctga
    18901 tcgccatatt tctccattag catctgcatc cccatcatat cgagcatcgg gtccatagag
    18961 agttgcagct tgcgtaccgt cagcccttcc agcgaaggta acgcaggcag gctacttaat
    19021 gtgtctggca aagcaccgga ggcactaata gcaatcggct gaatccgcat taccggatga
    19081 ggcttatcaa acggcgcaat cgccatcccc atctggctga ccggcagcgt caccaggtca
    19141 aagggtttgt tatcgttaac ctccaccagc acttcaaaac gctcgcccat cagcaccggc
    19201 agttcgctca ccttcactgg ttcaggtagc agaccaccgt cgctggcaat cacatacagc
    19261 gggcgattgt cgctggtggc gaaattgagc gaacgggcat tacagccatt gagcaaacgc
    19321 aggcgcagcc aaccacgcgg ggcagcgtgt tgcgggtaga ttgcaccgtt ggtcagcaac
    19381 gtatcgccaa accagcccac ggcggcggtc atcacatcca gttgataatc aatctgcccg
    19441 tcggcgctaa atttcttatc ctgaacgatc accggaacat catcgatacc ccactgtttt
    19501 ggcagcatta atttcaggat ctcgtcatct tcaatcacca ccagcccagc cagccccatc
    19561 gccacctgtc gcccggtttt gccgtgctga tgcggatgga accagcaggt agcggcaggt
    19621 tgatcaacgt tcaacgtcac cgagcgcttg ccacctggcg gaataattcc ctgcgggccg
    19681 ccgtcgactt cacccggtac ttccagcccg tgccagtgca acgttgtctc ttccgtcagt
    19741 tggttgtaga tatcaaccgt taccgctttg ccgcgctgta atttcaccgc cggccccagc
    19801 agattgccgt tatagcccca ggtagttgca gttttcccgc caaaggtgga ctggcctgcg
    19861 ccaatagtta actgaatgcg attacgggca tcggtcgtga gcaaatcagg gatcggtaac
    19921 gttgggcgtt ctgccgcaaa tactgcgcgg ctccacagcg gcaaagccga agccacaccc
    19981 agcgcgacgg aatattttaa gaaatcacga cgttgcatag ttatttcctt attcttaagc
    20041 aggcgaaaca aaatcaaacg ttgagcatag tccttcccct tacgggaagg tcaagccgca
    20101 gacacaattt aatcgacaat aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct
    20161 tctttaagcc cctggtagaa gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc
    20221 tgatggccgt ctgcgcaaac agttacgcgc tcagcgagtc tgaagccgaa gatatggccg
    20281 atttaacggc agtttttgtc tttctgaaga acgattgtgg ttaccagaac ttacctaacg
    20341 ggcaaattcg tcgcgcactg gtctttttcg ctcagcaaaa ccagtgggac ctcagtaatt
    20401 acgacacctt cgacatgaaa gccctcggtg aagacagcta ccgcgatctc agcggcattg
    20461 gcattcccgt cgctaaaaaa tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct
    20521 acgtcaaata atccctgata cctttttgta gaaatattga ccgtgcatcc gcggtcaatg
    20581 ttagctatta tgttgcgccc tttttttacg ggtgttaaca aaggaggtat caacccatgg
    20641 ccgaaaaaaa acagtggcat gaaacgctac acgaccagtt tgggcagtac tttgcggtag
    20701 ataacgttct gtatcatgaa aagaccgatc accaggatct gatcattttt gagaacgctg
    20761 catttggtcg cgtaatggcg ctggatggcg tagtacaaac caccgagcgc gacgagttta
    20821 tctatcatga gatgatgacc catgttccgc tactggccca tggtcacgcg aaacatgtgc
    20881 tgattatcgg cggcggcgac ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg
    20941 agtcaatcac gatggtggaa atcgatgcgg gtgtcgtatc gttctgccgt cagtatctac
    21001 ccaaccataa cgccggtagc tacgacgatc cgcgctttaa gctggtgatc gacgatggcg
    21061 tcaatttcgt taatcaaacc agccagacct ttgatgtcat tatctccgac tgcaccgatc
    21121 ctatcggtcc cggcgaaagc cttttcactt cggcatttta tgaaggctgc aaacgttgcc
    21181 tgaatcctgg cggtatcttc gtcgcacaaa acggcgtctg ctttttacag caggaagaag
    21241 ccatcgacag ccatcgcaaa ctcagccatt acttcagcga cgttggcttt tatcaggcgg
    21301 cgatcccgac ctattacggc ggtatcatga cttttgcatg ggcgacagat aacgacgcct
    21361 tacgccatct ctcaaccgaa attattcagg cgcgttttct cgcctctggc ctgaaatgcc
    21421 gttattacaa tccggcaatc catacggcag cttttgcctt acctcagtat ctgcaagacg
    21481 cactggcttc acagccgtcc taaggagaag ataagaaatt gaaaaaactg aaactgcatg
    21541 gctttaataa tctgaccaaa agtctgagtt tttgtattta cgatatctgc tacgccaaaa
    21601 ctgccgaaga gcgcgacggt tatattgctt atatcgatga actctataat gccaaccgtc
    21661 tgaccgaaat cctgtcagaa acctgttcca ttatcggggc taatattctt aacatcgccc
    21721 gccaggatta cgaaccacag ggtgccagcg tcactattct ggtgagtgaa gaaccggttg
    21781 acccgaaact catcgacaaa acagaacacc ccggcccact gccagaaacg gtcgttgccc
    21841 atcttgataa aagtcatatt tgcgtacata cctacccgga aagtcatcct gaaggcggtt
    21901 tatgtacctt ccgcgccgat attgaagtct ctacctgcgg cgtgatttct ccgctgaagg
    21961 cgctgaatta cctgatccac cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc
    22021 gcggttttac ccgcgacatt aacggtatga agcactttat cgaccatgag attaattcga
    22081 ttcagaactt tatgtctgac gatatgaagg cgctgtatga catggtggat gtgaacgtct
    22141 atcaggaaaa tatcttccat accaagatgt tgcttaaaga gttcgacctt aagcactaca
    22201 tgttccacac caaaccggaa gacttaaccg acagcgagcg ccaggaaatt accgctgcgc
    22261 tgtggaaaga aatgcgcgag atttattacg ggcgcaatat gccagctgtt taacggctct
    22321 ggcggagctc ccaggctccg ccagatttat ttacttctgc tgcacgaaat tgcggtaagc
    22381 cgccacgacc tgcagaaaat cctcaacgcc gcatagcgac aggctttctt cgtcgtagta
    22441 gttcatcccc tcttccattt catcgccagc gaattccagt tgattggcgc gaaccatcac
    22501 ctcttcaccg tccatccaca gggtgtattc atgccctgcc cgttgccagg accgttcgct
    22561 acctttcagt gcgtgcgctg cttgttccac ttcatcaagc aaggccaggt tttctttcac
    22621 ctcttcatta aaccagtgcc cgaccacttc atgccccatg gacatacgca cctttaccac
    22681 tccggtaata tcgcgcagaa attcgtaatc catagtgtgt tcctctgctc ccggcaatgc
    22741 accgaaatcg cattgcgcca ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg
    22801 aacggcgatg gtttagaaat aaaaaaatgc ccgggaatct cattcaccgg gcattgtgtc
    22861 gtttatgcgc agcgcgtgcg ctgacttttt aaaccgcagt ctggaaaatc accccatcgg
    22921 ctttctcggt gtactgagaa agctggttga agttcagata acggtaagta tcaacggctg
    22981 ttttatctac ctgcgccacg taggtctggt actcttccgg cgtcggcagt ttgccaatca
    23041 gcgccgcaac agccgccagt tccgcagaag ccaggaagac attcgcgcca gtacccagac
    23101 ggttcgggaa gttacgggta gaggtggaaa ccaccgttgc accgtccgcc acacgcgcct
    23161 ggttacccat acacagggaa cagccaggga tctcgatacg cgcaccactc ttaccgaaga
    23221 cgctgtagta gccttcttcg gtcaactgtg cggcgtccat acgggttggc ggtgccaccc
    23281 acaggcgggt cggcaactga cctttatgcg catccagcag tttacccgca gcacggaagt
    23341 gaccgatgtt ggtcatgcag gaaccgataa acacttcgtc gatcttctca ccctgtaccg
    23401 cagacagcgg acgcgcgtca tccgggtcgt tcggagcaca caggattggc tctttaatat
    23461 ccgccagatc gatgtcgatc actgccgcgt attccgcatc tgcatcggct tccagcagct
    23521 caggattcgc cagccatttt tccatgccct gaatacgacg ttccagggta cgacgatcgc
    23581 cgtaaccttc cgcgatcatc cacttcagca ggacgatgtt agagttcagg tattcgatga
    23641 tcggttcttt gttcagcttg atggtacaac cagcggcaga acgctcggcg gacgcatcgg
    23701 ttagctcaaa ggcctgctca actttcagat ccggcagacc ttcaatttcc aggatgcggc
    23761 cagagaagat gtttttcttg cctttcttct caacggtcag cagaccttgt ttgatcgcat
    23821 acagcggaat agcgtgtacc agatcgcgca gggtgatgcc cggctgcatt ttgcctttga
    23881 agcgcaccag aacggattcc ggcatatcaa gcggcattac gccagttgcg gcagcaaacg
    23941 ccaccagacc agaacccgcc gggaaagaga taccgatcgg gaaacgggta tgggagtcac
    24001 caccggtacc gacggtatcc ggcagcagca tacggttcag ccaggagtga atgacgccgt
    24061 cacccggacg cagcgacaca ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg
    24121 tgttcacgtc aactggcttc ggatacgccg cggtgtggca gaaagactgc atcaccaggt
    24181 cagccgagaa gcccaggcac gccaggtctt tcagttcatc acgggtcatc gggccggtgg
    24241 tgtcctggga acctacagaa gtcattttcg gttcacagta cgcgcccgga cgaatgcctt
    24301 tcacgccaca ggcacggcct accatttttt gcgccagcga gaagccgcga tcgctctcag
    24361 cgacatcttt cgcctgacgg aacacatcac tgtgcggcag accaagtgct tcacgcgctt
    24421 tggtggtcag gccacgcccg ataatcagcg gaatacggcc accagcacgc acttcatcaa
    24481 tcagcacgtc ggttttcagt tcgaaggtcg ccagcagttc gccggtttcg tggttacgca
    24541 cttcaccttt gtacgggtaa acgtcaatca cgtcgcccat gttcaggtta gagacgtcga
    24601 cttcgattgg cagtgcaccc gcgtcttcca tcgtgttaaa gaagatgggt gcaattttac
    24661 cgccgaggca caaaccaccg ccgcgtttgt tcggcacatg tggaatatca tcgcccataa
    24721 accacagaac ggagttagtg gcggatttac gcgaagaacc cgtacccaca acgtcaccga
    24781 cgtacgccag cgggaaacct ttctgttgca gagcttcgat ttgcttgatc ggaccaacaa
    24841 caccaggctg gtctggctca ataccttcac ggacgttttt cagcatcgcc agcgcgtgca
    24901 gtgggatatc cgggcgtgac cacgcatccg gtgccggaga aaggtcatcg gtgttagttt
    24961 cgccagtgac tttgaagaca gtaacggtca gtttttcagc cagcgccggg cgattcagga
    25021 accattcggc atccgcccag gactgcataa cctgcttcgc atattcgttg cctgctttcg
    25081 ctttctcttc tacgtcatag aagttatcga acatcagcag cgtgtgagaa agtgctttgg
    25141 cagcaatagg tgccagtttg gcatcatcca gcgcgtcgat cagcggatga atgttgtaac
    25201 caccctgcat ggtgcccagc agttcgatgg ctttttccgg agtcagcaga ggggatttgg
    25261 cttcgccttt cgcgatagca gccaggaagc ctgctttgac ataggcggct tcatcgacgc
    25321 ctgggggaac acggttggtt aacagatcta acaggaattc ttcttcgccc gcgggcgggt
    25381 ttttcagcag ctctacaagt gcggccattt ggtttgcatc caggggtttg ggcgcaatcc
    25441 cctcagcggc acgctcagct acgtgcttac ggtattcttc tagcacgacg gttctcctcg
    25501 ctctcattgt catagtgcgg caggcgattc tcttcacgct cctgtgagac agcagtttgt
    25561 agggtaaatg cccagtaccg caacgggcag aatagcagga ttttgacgag gtgttaatct
    25621 gtttacaaaa aagcaacata aaaaagtggc tgaatcgtta aggatggtct agagattgtt
    25681 tccctccgta aaaacttcgt taattcgcat ggtgataatc acctttctca acgaatccca
    25741 agattagtca aaatttaaac taccgcctct ttatactcgg attcacagca cctgcgggtg
    25801 gcagttcgcc gaccattgcg atttccttga gatccgaatt atgaaaatga ctttgccgtt
    25861 taaaccccat gtgctggcgc taatttgcag tgccgggctt tgtgccgcct ctaccgggct
    25921 atatataaaa agccgcacag tggaagcgcc tgtggaaccg caatcgacac aactggctgt
    25981 gtctgacgct gccgcggtta cgtttcctgc aacggtttcc gcacctcccg taacacccgc
    26041 cgtcgtcaaa tccgcattca gcactgcaca aatagatcaa tgggtcgcgc ccgtcgcgct
    26101 gtatcccgac gccctacttt cgcaggtgct gatggcatca acctatccga caaacgttgc
    26161 tcaagcagtg caatggtcgc acgataatcc acttaaacaa ggcgatgctg ctattcaggc
    26221 ggtatctgac cagccgtggg acgccagcgt taaatcactg gtggcctttc cacaattgat
    26281 ggcattgatg ggcgaaaacc cgcaatgggt gcaaaacctg ggcgatgctt ttctggccca
    26341 gccgcaggac gtgatggact cggtacagcg attgcggcaa ctggcgcaac aaaccggctc
    26401 gctgaagtca tcaaccgaac agaaagttat taccacaacg aagaaagctg taccggtaaa
    26461 acagacagtc acggcacccg tcataccatc caatacagtt ttaactgcca accccgtcat
    26521 tacagagccg gcaacaaccg tcatttccat tgagcccgcc aatcctgatg tggtctatat
    26581 tcccaactac aacccaaccg tggtttacgg gaactgggcc aatactgcgt atccgccggt
    26641 ttatctgcca ccaccagccg gagaaccgtt tgttgacagc tttgtacgcg gattcggcta
    26701 tagcatgggc gttgctacca cgtacgcact attcagcagc atcgactggg acgacgacga
    26761 tcatgaccat catcatcatg acaatgatga ttatcatcac cacgatggcg gtcatcgtga
    26821 cggtaatggc tggcaacaca acggcgacaa catcaatatc gacgtcaaca atttcaaccg
    26881 tatcaccggt gagcatctta ctgataagaa tatggcatgg cggcacaatc caaactaccg
    26941 taatggtgtg ccctatcatg atcaggatat ggcaaagcgg tttcatcaaa ccgatgtcaa
    27001 cggcggaatg agcgccacgc aattacctgc cccaacgcgc gacagccagc gtcaggcggc
    27061 agcaaatcag tttcagcaac gaacacacgc cgcaccagtc attacacgag atacccaacg
    27121 tcaggcagcg gcacagcggt ttaatgaagc tgaacactat gggagctatg acgacttcca
    27181 cgacttcagc cgtcgccaac cactgaccca gcaacaaaag gacgccgctc gtcagcgtta
    27241 tcagtccgcc tcgcctgagc agcgccaggc agttcgcgag agaatgcaga ctaacccgaa
    27301 gatccagcag cgaagagagg cagcgcgtga gcgcattcag tccgcctcgc ctgagcagcg
    27361 ccaggcggtt cgcgagaaaa tgcagactaa cccgcagaac cagcagcgaa gagacgcagc
    27421 gcgtgagcgt attcagtccg catcaccaga gcagcgccag gtgtttaagg aaaaagtaca
    27481 gcagcgccca ctgaatcaac agcaacgtga taacgcccgc cagcgtgttc aatcggcatc
    27541 acctgaacaa cgtcaggttt ttcgggagaa agttcaggag agccgcccac aacgtctaaa
    27601 cgacagtaac catactgtca ggctgaataa cgagcaacgg tcagcagtat gcgaacgtct
    27661 ctctgaacgt ggagcaagaa gactggaaag gtaaattgca gacgtaaaaa aagcggcgtg
    27721 gttaaccgct tttttaattg ccggatgttc cggcaaacga aaaattactt cttcttcgct
    27781 ttcgggttcg gcaggtcggt aatgctacct tcgaacactt ctgccgccag gcccacagac
    27841 tcgtgcagag tcgggtgcgc gtggatggtc agtgcgatgt cttcagcatc acaacccatt
    27901 tcgattgcca ggccgatttc acccagcagc tcgccgccgt tagtaccgac aatcgcacca
    27961 ccgatcacac ggtgagattc tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg
    28021 gaagcgatag cacgaccaga agcagcccac gggaaggtgg cggtttcata gctgatgcct
    28081 ttctctttcg cttctttctc agtcagaccc acccatgcaa cttctggttc ggtataggcg
    28141 atggacggga taactttcgg atcgaagtag tgtttcttac cggcgataac ttcagcggca
    28201 acgtgacctt cgtgaacacc tttgtgtgcc agcatcggtt gaccgacgat atcgccgata
    28261 gcaaagatgt gcggtacgtt ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg
    28321 tcaacttcca cgcctgcttt gcctgcgtcg aggtttttac cgttcggcac acgaccaatc
    28381 gctaccagca cggcgtcgta acgctgcggt tcagcgggtg cttttttgcc ttccatcgtc
    28441 acataaatgc cgtcttcttt cgcttcaacg gcggtaactt tggtttccag catcaggttg
    28501 aatttcttgc tgatacgctt ggtgaagact ttaacgatgt ctttgtcagc tgccgggata
    28561 acctggtcga acatttcaac cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc
    28621 atttccagac cgatgatacc gccacccatt accagcaggc gttctggtac ttctttcagt
    28681 tccagcgcgt cagtggagtc ccagatacgc ggatcttcat gcggaataaa cggcagttgg
    28741 atcgggcgag aacccgctgc aatgatcgcg ttgtcgaagt tgatcacggt tttgccgttc
    28801 tcaccttcaa cttccagggt gttagccccg gtgaatttac ccagaccgtt gaccactttg
    28861 actttgcggc ctttcgccat accagccaga ccaccggtca gctgattgat cactttctct
    28921 ttccaggtac gaatcttgtc gatatcggtt ttcggttcgc cgaagacgat accgtgttca
    28981 gccagcgctt tggcttcttc gataactttt gctacgtgca gcagtgcttt agaagggata
    29041 cagccgacgt tcaggcaaac accgccaagg gtgttgtaac gttctacgat tacggtttcc
    29101 agacctaaat cagcgcaacg gaaggcagcg gagtaacctg cggggcctgc cccaagtacc
    29161 acgacctgag ttttgatttc agtactcatc atgacctcta tatatttatc tccggcggtc
    29221 atacccgtcg tctttcaggc cgcagcggcg ttagcttcac gccctcaccc cagtcactta
    29281 cttatgtaag ctcctgggga ttctggcgct tgctaccttg ctgcacccag aaatccatag
    29341 ggtatgtgaa gtaataccct aaccaccacc gggtcgttct atccgtcggt attttacaaa
    29401 attgttaaca atttttaaac aacaaacggc aaccgatttg tctattcgct aataacctca
    29461 atacattcat gagattacca gaaaaaagcc ggccgttggg ccggctcttt tacttacatc
    29521 accagacggc gaatgtcaga cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca
    29581 gcaccgtcga tcacgcggtg gtcgaaggag agagaaatcg gcagcatcag acgcggcacg
    29641 aactctttac cattccacac cggctccatc gcggacttgg aaacgccgag gatagccact
    29701 tccggcgcgt tcacaatcgg cgcgaagtgg gtagtaccca ggccgccgat gctggagatg
    29761 gtgaagcaac cgccctgcat ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa
    29821 atagtcatca gctcgcgaga cagctcgatg atgcctttct tgttgacgtc tttgaatacc
    29881 ggaacaacca gaccgttcgg ggtatccacc gccacaccga tgttgatgta tttcttcagg
    29941 gtcagacgct gaccgtcttc cgacagcgaa ctattgaagc gaggcatctg ctcaagagct
    30001 gcagcaacgg ctttcatgat gaagacaacc ggggtgatct tcacatccag cttacgtttc
    30061 gccgcttctt cgttctgctg tttacggaac gcttccaact cggtgatatc ggttttgtcg
    30121 aagtgagtaa catgcgggat cattacccag ttacggctca ggttcgcacc agagattttc
    30181 tggatgcggc ccagttccac ttcttcgatt tcaccaaact tgctgaagtc caccttcggc
    30241 cacggcagca tgccagggat accaccgcca gtcgctgccg gagctgcttc tgcacgtttg
    30301 atagcttctt tcacgtaagc ctgaacgtct tcgcgcagga tacgaccttt acggccagtg
    30361 cccttcactt tcgcaaggtt aacaccaaac tcgcgtgcca gacggcggat cagcggagtc
    30421 gcgtgaacat aagcgtcgtt ttcagcaaat tcagatttgc cttccgcttt cgcagctggt
    30481 gctgctgccg gggcttcagc ttttgctgcc ggtgccggcg ctgccgcttc ctgtttcgca
    30541 ggagctgccg caggcgctgc gccttcaact tcgaagatca taatcagcga gccagttttc
    30601 actttatcgc caacgttgac tttcagttcc ttcacgacgc ctgcaaacgg cgccggaact
    30661 tccatagaag ctttgtcgcc ttctacggtg atcagtgact gttcagcggc aactttgtcg
    30721 cccactttca ccatcacttc agtcacttca acttcgtcac cgccgatatc cggaacgtta
    30781 acttctttca cgccagccgc tggtgcaggg gccgctgccg gagctgcttc ctgtttagcg
    30841 gccggagctg ccgcgcctgc ttcacccgcg acttcgaaga ccataatcag cgagccggta
    30901 gacactttgt cacccacgtt cactttgatc tctttcacgg tgccagcaaa cggagccgga
    30961 acttccatag aagccttgtc gccttctacg gtgatcagcg actgttcagc ttcaacttta
    31021 tcgccaactt tcaccaggat ttcggtcact tcaacttcgt cgctgccgat atccggaacg
    31081 ttaacgtctt ttgccgccgc agccgctggt gctgctgccg gagctgcttc tttcttctct
    31141 tctgcctgag caggtgcagc gtctgctgca ccgtcggcgg aatcgaaaat cataatcagt
    31201 gcgccggtct gggttttatc gccaacagag actttgatct ctttaacgat acccgcctgc
    31261 ggagacggaa cttccataga ggctttgtcg ccttctacgg tgatcagcga ctgttcggct
    31321 tcaactttgt cgcccacttt gaccaggatc tcggtgattt caacttcatc agccccgatg
    31381 tccggtactt tgatttcgat agccattatt cttttacctc ttacgccaga cgcgggttaa
    31441 ctttatctgc atcgatgttg aatttggcga ttgcgtcagc aaccactttc ttatcgattt
    31501 cgccacgttt agccagttcg cccagcgccg caaccacgac ataagaagca tcaacttcga
    31561 agtggtgacg caggttctca cggctgtcgg aacgaccgaa gccatcagta cccagtacgc
    31621 ggtagtcgtc agccggtacg taagtacgga cctgctcagc gaacagtttc atatagtcgg
    31681 tagatgccac tgccggagcg tcgttcatca cctgagcgat atacggaacg cgcggagttt
    31741 ccagcgggtg cagcatgttc cagcgttcac aatcctgacc atcacgcgcc agctcggtga
    31801 aggaggtcac gctataaacg tcagaaccta cgccgtaatc tttcgccagg atctcagctg
    31861 cttcacggac gtgacgcagg atagaaccgg agccgagcag ctgaacttta cctttgctac
    31921 cttcaatagt ttcgagtttg tagatacctt tacggatacc ttcctcagca ccttccggca
    31981 ttgccggcat gtggtagttt tcgttcagcg tagtgatgta gtagtaaacg ttctcttgtt
    32041 tttcaccgta catacgctcc agaccgtcat gcatgatgac agcaacttcg taagcgtaag
    32101 ccgggtcgta agagatacag ttcgggatag tcagcgactg aatgtggctg tgaccatctt
    32161 cgtgctgcag accttcgccg ttcagggtgg tacgaccgga agtaccgccg atcaggaagc
    32221 cacgcgcttg ctggtcgcca gccgcccagc acagatcgcc aatacgctgg aagccgaaca
    32281 tcgagtaata gatgtagaac gggatcatcg gcagattgtt ggtgctgtaa gaggtcgccg
    32341 ctgccagcca ggaacaacct gcgcccagct cgttgatccc ttcctgcaga atctgacctt
    32401 tctcgtcttc tttatagtaa gcaacctgct cgcggtcctg cggggtgtac tgctgaccgt
    32461 tcgggctgta aataccaatc tgacggaaca gaccttccat accgaaagta cgcgcttcgt
    32521 cggcgatgat cggtaccaga cgatctttga tcgacttgtt cttcagcatc acgttcagag
    32581 cacgaacgaa agcgatagtg gtagagatct ctttgctctg ctcttccaac agcgcgccga
    32641 agtcttgcag gctcggcagc tcaagcttct cggtgaagtt cggctgacgg cttggcagat
    32701 aaccgtgcag tttctgacgc tgagcgtgca gataggtatg ctcttcagaa ccttccggga
    32761 aggtgatgta cggcagtttt tcgatatctg catcagacac cggcacattg aaacggtcgc
    32821 ggatatgacg cacaccgtcc atgttcattt tcttaacctg gtgcgcgatg tttttacctt
    32881 cagccgcgtc gcccatgccg taacctttaa tggtatgagc aaggattact gtcgctttgc
    32941 ctttggtttc ctgcgctttc ttgaatgcag cgtagatttt cttcggatcg tgaccaccac
    33001 ggttcagtgc ccagatctgc tcgtcagtcc agtctgcaac cagtgctgcg gtttcaggat
    33061 atttaccgaa gaagtgttca cgaacgtacg caccatcttt cgatttgaag gtctggtagt
    33121 cgccgtcaac ggtttcgttc atcagctgga tcagtttacc gctggtatcc ttacgcagca
    33181 gttcatccca acggctaccc cacatcactt tgatcacgtt ccagccagca ccttcgaaga
    33241 tgccttccag ttcgttgatg atcttgccgt taccggtgac cgggccgtca agacgctgca
    33301 ggttacagtt gataacgaag accaggttat ccagtttttc acgggtagcg atggtgatcg
    33361 cacctttgga ttccggttcg tccatttcac cgtcaccgag gaacgcgtaa acggtttgtt
    33421 tagaggtatc tttcaggcca cggtgttcca gatatttcag gaatttagcc tggtaaatag
    33481 caccaatcgg acccagaccc atagatacgg tcgggaactg ccagaattcc ggcatcagtt
    33541 tcgggtgcgg ataggaagag aggccattgc cgtgaacttc ctgacggaag ttatccagct
    33601 gctcctgagt cagacgacct tccaggaaag cacgagcgta cacgcccggg gagatgtggc
    33661 cctggaagta aaccaggtcg ccgccatcct gctcgttgcg tgcacggaag aagtggttaa
    33721 agcacacatc ataaatggtt gcggaagact ggaaggacgc catatggccg cccagttcga
    33781 ggtctttttt cgacgcacgc agcaccgtca tgatggcgtt ccagcggata gctgaacgaa
    33841 tacggcgttc cagttccaga ttacccggat actccggttg ttcttcaacg gggatggtgt
    33901 tgatgtagtt gctgatacct gtgcctgcgg ctacgtttac accgcctttg cgggcttcag
    33961 caagcagttg gtcgatcaga tactgagcac gctcaacacc ttcttcacgg atgaccgatt
    34021 cgatcgcctg gagccagtcg cgagtttcga tcggatccac gtcatttggg aaacgttctg
    34081 acatgggtta ttccttatct atctaataac gttgagtttt ctggaacctg tcccattgaa
    34141 ctctcgccgg aaagctcaat aagacaggtt ctacgtttag ttgccgcgct tttatatgcg
    34201 cttgatttac aacatcttct ggataatttt taccagaaaa atcactaatt ctttcgttgc
    34261 tccagacgac gcagagaacg ctcacggcgg ctctcttcac gacttctgtc gagcaaaatt
    34321 tcttcgataa aggccagatg gcgatgcgat gcttcgcgcg cttcttccgg cttaccggcc
    34381 ataatcgctt caaatatgcg ggtgcggtga ctactcacca gcggcagcat ctcgcgacgc
    34441 gaatagagca attcgaagtt ctggcggaca ttctgggcca acatcggctc catacacctt
    34501 agcagatgaa gcagaaccac attgtgggcc gcttcggtga cggcaatctg atactggagt
    34561 acggcgtttg attccgcgtc cagatcgcca gactgctgcg ccagctctat ggcgtggtgg
    34621 agttcacgga tgcgttcctt gtcttcatcg gtactacgca gcgcggcgta ataagcggcg
    34681 ataccttcca gggcgtgtcg tgtttcgagc aagtcatact gtgactcagg atggtcggag
    34741 agcagctcca ccagcggatc gctgaagctt tgccataggc tgctctggac aaaagtgccg
    34801 ccaccctggc gacgaagcaa caagcccttc gcttcgagac gttgaatcgc ctcacgcaag
    34861 gagggacggg agacgtcaaa ctgttttgcc agttcgcgtt ccggtgggag tttttcgccc
    34921 gggcggagag tgccttcgag gatcaaaaac tccagttgct gctcaatcac atcggagagt
    34981 tttggttggc ggattttgct gtaggccatg agttcctgtc ttaagccact tgccgaagtc
    35041 aattggtctt accaatttca tgtctgtgac gctaaagtaa caaagtattc accttatgtc
    35101 catacaggtt ttgattgaaa tcatgaaact gtgcacattt taacaacttg acatatataa
    35161 cgtttcaaag ttgtaactat gcacaaatgt agactttacg taggaaagga gttttttaac
    35221 gattattaaa ctataaaaat ccgaattgaa ccgattcact taccaatttt gtgattttta
    35281 attcaattaa aacgaattta aattcattct acatattgag aggggttgag gctgagcttt
    35341 acaaacggtt tctttttaag caactcatct tcaaccatgc ataaagcggg tgcattcgct
    35401 gccgcatacc attattcttg atctgacgga agtctttttg taacaattca aacttctttg
    35461 atgtaaacaa attaatacaa caaacggaat tgcaaactta cacacgcatc actgcgtaga
    35521 tcaaaaaaac aaccaccgca cgaggtttca tgatggaagg tcaacagcac ggcgagcagc
    35581 taaagcgcgg ccttaaaaac cgccatattc agcttatcgc gctgggtggc gcgataggga
    35641 ccgggttatt cctgggtagc gcctccgtaa tacagtccgc agggccaggg attatcctgg
    35701 gttacgccat tgctggtttt atcgcctttc tgatcatgcg tcagctgggt gaaatggtgg
    35761 tcgaagaacc tgtcgcaggc tcctttagcc actttgctta taaatactgg ggcagttttg
    35821 ccggtttcgc ctctggctgg aactactggg tactgtacgt tttagttgcc atggctgagc
    35881 tgactgccgt gggtaaatac attcagttct ggtatccgga aatccccacc tgggtttctg
    35941 ccgccgtatt ctttgtggtg attaacgcca tcaacctgac caacgttaaa gtgtttggcg
    36001 agatggagtt ctggtttgcc attatcaaag ttatcgcggt ggtagcgatg atcatcttcg
    36061 gcggctggct gctattcagt ggcaacggcg gcccgcaggc gaccgttagc aacctgtggg
    36121 atcagggtgg tttcctgccg cacggcttca ccgggctggt gatgatgatg gcgattatca
    36181 tgttctcgtt cggtggtctg gaactggtgg ggatcaccgc agcagaagct gataacccgg
    36241 agcaaagtat accgaaagca actaaccagg ttatctaccg catcctgatt ttctatattg
    36301 gttcgttagc cgttctgctc tcactgatgc cgtggacccg cgttaccgcc gataccagtc
    36361 cgtttgtgct gatcttccac gagttaggcg atacctttgt ggcgaatgcg ctgaacatcg
    36421 tggtactgac tgcggcgctc tccgtgtaca acagctgcgt atattgcaac agccgtatgc
    36481 tgtttggtct ggcacaacag ggtaatgcgc caaaagcgct ggcgtctgtc gataaacgtg
    36541 gtgtaccagt aaataccatt ctggtgtctg cactggtaac ggcgttgtgc gtactgatta
    36601 actaccttgc cccagagtcc gctttcggac tgttaatggc gctggtggta tctgcactgg
    36661 taatcaactg ggcgatgatt agcctggcgc atatgaaatt ccgtcgcgcc aagcaggaac
    36721 aaggcgtggt aactcgcttc cctgctctgc tttatccgct gggtaactgg atctgcctgc
    36781 tgtttatggc ggcggtactg gtgattatgc tgatgacccc aggaatggcg atttcggtat
    36841 acctgatccc ggtatggctg atcgtgttag gtatcggcta tctgtttaaa gagaaaaccg
    36901 ccaaagccgt aaaagcgcat taatctctct acgccctcac ccgtacaggg tgagggcaat
    36961 aatctttaca ccaacgcccc gtaaatcgtc agtagtgcaa tcaccaccac gaccacgaac
    37021 gaggttttct tcgccattga aaccgctgcc ttcggcgtct ccaccttatc gacatgcggt
    37081 tcacgcgcca gagagaactg cgccagacgc gttaacacct gatactgcga agtatggaaa
    37141 tcacccagcg aagcaaacca ggccggtaac gctttctcac catgaccgat caaggcatat
    37201 accacacccg caagacgaac cggcacccaa tccagtacat gaagcacggc atcaatgccg
    37261 gactgtaaac gatgatgcgg cgtctgatat cgcgccagcc agtattgcca tgcacgcaaa
    37321 aacgcatacc ccatcagcgt aacgggtccc caggttcccc ccacaatcag ccagaacagc
    37381 ggtgcaagat aaaaacgaaa gttaatccac agcaatgcat tttgcagctc acgcaaatac
    37441 tcacgttcgt cgcagcctgc cgggacgccg tgaatcatgg tgagttcgcc agccatcgtg
    37501 gcacgggcat ggctatcatt acgtgaagca gctgtcagat aagcatgata atgaagacga
    37561 actttacctg cgccaataca cagcaaacca atcagcagcc acaccagtag cgtgggaacg
    37621 ttgaacaata ctccctgcaa tgcgcgtaac agtaaaaaag tcacgcccat cgcaataatg
    37681 gtcatgccta acgtgcgccc gagagaaaaa tgtttcaccc gccgaaagaa cgcttcaaga
    37741 cgatgatcaa gctgccagtg ctcgcccaac ttaaacaggc gctcgaaaat taacaccagt
    37801 aaggttgtaa atagcgtcat gttgtctcct tgctgaccag cacacgaaac cgtgcccaat
    37861 caaatgcagg accgggatcg gttttccgat ccggcgcaat atcacaatgg cccgtcatgt
    37921 ttttagcgat atccggatag caatcaatca gtgcccgcgt aaccgccgca agctgttgat
    37981 actgcgcatc ggtatacgcc agcgtatcgg tgccttcaag ctcaatccca atagaaaaat
    38041 cattgcagcg ttcgcgcccc tgatactgag agactcccgc atgccatgca cgtttatcga
    38101 aaggaacata ctggactatt tcaccatcac ggcgaatcaa acagtgagcg gagacgcgca
    38161 aatgggcgat ctcagcaaag aaaggatgtg cctgcggatc aatagttcca gtgaataatg
    38221 cgtcgatcca cggaccgcca aactcgcctg gcggcaggct aatattgtgc accaccagca
    38281 gggtgggtgt ttcgtcatcc gggcggcaat cgtaatgtgg tgagggaacg cggcgcgcgc
    38341 caaccagcca cccctgttct aacaacatgc aggatctcct tatatgtggt gctaataccc
    38401 ggttcagagt agcatgtttc taccttatga ttcgttagct atctggagtt ttaacatgcc
    38461 gcctcgccgc tataaccctg acacccgacg tgacgagctg ctggaacgca ttaatctcga
    38521 tatccccggc gcggtggccc aggcgctgcg ggaagattta ggcggaacag tcgatgccaa
    38581 caatgatatt acggcaaaac ttttaccgga aaattctcgc tctcatgcca cggtgatcac
    38641 ccgcgagaat ggcgtctttt gcggcaaacg ctgggttgaa gaggtgttta ttcaactggc
    38701 aggcgacgat gtcaccataa tctggcatgt ggatgacggc gatgtcatca atgccaatca
    38761 atccttgttc gaacttgaag gcccatcccg cgtgctgtta acgggcgaac gcactgcgct
    38821 taattttgtg caaacccttt caggagttgc cagtaaggta cgccactatg tcgaattgct
    38881 ggaaggcacc aacacgcagt tgttggatac gcgcaaaacc ttacccggcc tgcgttcagc
    38941 tctgaaatac gcggtacttt gcggcggcgg agcgaatcac cgtctggggc tttctgatgc
    39001 cttcctgatc aaagaaaacc atattattgc ctccggctca gtgcgccagg cggtcgaaaa
    39061 agcgtcctgg ctgcacccgg atgcgccagt agaagtcgaa gtagagaatc tggaagaact
    39121 tgatgaagcc ctgaaagcag gagccgatat catcatgctg gataacttcg aaacagaaca
    39181 gatgcgcgaa gccgtcaaac gcaccaacgg caaggcgcta ctggaagtgt ctggcaacgt
    39241 cactgacaaa acactgcgtg aatttgccga aacgggcgtg gactttatct ccgtcggtgc
    39301 gctaactaaa cacgtacaag cactcgacct ttcaatgcgt tttcgctaat agttgacaga
    39361 tttatccgct ccatcgcggg cggataatct ccccttcccc aactttcttc gtaacgcctc
    39421 gcaaatttct tgtctttcgc tgcaacgaaa taaaaaacct cggaatctgc tttcccgttt
    39481 cgttttttgc ccctcgcctg tgctgatggc gtttgccaaa gtagcgccaa ccaaatcaag
    39541 gagcgaaaca gatggacaag caacgcggtt ttacacttat cgaactgatg gtggttattg
    39601 gcatcattgc cattttaagc gccattggta ttcccgctta tcaaaactac ctgcgcaaag
    39661 ccgcactcac cgacatgcta caaacctttg tgccttaccg taccgccgta gagttgtgcg
    39721 cgctggaaca tggtggatta gatacctgcg acggtggcag caatggcatt ccctcgccta
    39781 ccaccacccg ctatgtttca gccatgagtg tggcaaaggg cgtggtgtcg ctgaccgggc
    39841 aagaaagtct caatgggcta agcgtcgtca tgacaccggg ttgggataac gcaaacggcg
    39901 tcaccggctg gacgcgcaac tgcaatattc aaagtgacag cgcattgcag caagcctgcg
    39961 aagatgtctt ccgctttgat gacgccaact aaggagcggc aatgaatatt ccacagctca
    40021 ctgccctgtg tctgcgttat catggagtct tgctggatgc cagcgaagag gtggttcatg
    40081 ttgcggtagt cgatgcacct tcgcatgagc tactggacgc attgcatttc gctaccacca
    40141 aacgtattga gatcacctgc tggacgcgcc aacaaatgga aggtcacgcc agtcgcacac
    40201 aacagacatt gcccgtagct gttcaggaga agcatcagcc caaagcagag ttgctaactc
    40261 gaacgttaca atctgcgctg gaacaacgcg cgtctgatat tcatatcgaa ccagcggaca
    40321 atgcctaccg catccgcttg cgtatcgacg gcgtattgca tcctttaccg gatgtttcac
    40381 cggatgccgg agtcgcatta accgccagat taaaagtgct gggaaacctg gatattgcgg
    40441 aacatcgcct gccgcaggac gggcaattca ctgtcgaact ggcaggaaac gccgtctcat
    40501 ttcgtattgc gaccttacca tgtcggggtg gtgaaaaggt ggtattaagg ttgttacagc
    40561 aggtgggtca ggcactggat gtcaacacgc ttggaatgca gccgttacaa ctggcggact
    40621 ttgctcatgc cttgcaacaa ccacagggac tggtgctggt aactggccct accggcagcg
    40681 gcaaaacggt cacgctttat agtgccctgc aaaagctgaa taccgctgac attaatattt
    40741 gtagcgtcga agatccagtt gagatcccca tagccggact aaaccagacg caaatccatc
    40801 cgcgtgccgg actcaccttt cagggcgttt tgcgtgcgtt attgcgccag gatcctgacg
    40861 tcatcatgat cggagagatc cgcgatggcg aaacagcaga gatcgctatt aaagcggcgc
    40921 aaactggtca cctggtgttg tctaccctac acactaattc cacctgcgaa acgctggtac
    40981 gtttacagca aatgggggtc gcccgctgga tgctatcatc ggcgcttacg ctggtaatag
    41041 cccagcgtct ggtacgcaaa ctttgcccac attgtcgccg gcagcaaggg gagcccatcc
    41101 acattccaga caatgtatgg ccatcgccgc tgccccactg gcaggcaccc ggttgtgtac
    41161 attgctacca cggtttttat ggtcgtacgg ccttatttga agttctgccc ataacgccgg
    41221 tcattcgtca gcttatttcc gctaataccg acgttgaatc gctggaaacg cacgcacgac
    41281 aggcgggtat gcgtacgctt tttgaaaacg gctgcctggc cgtagagcaa ggcttaacca
    41341 cctttgaaga gttaatccgc gtactgggga tgccgcatgg cgagtaagca actctggcgc
    41401 tggcatggca taaccggcga cggcaatgcg caagatggga tgctatgggc agagagccgt
    41461 actttactgc ttatggcact acagcaacag atggttaccc cactaagcct gaagcgaatc
    41521 gccatcaatt ctgcgcagtg gcgaggagat aaaagcgcgg aagtcattca tcaactggcg
    41581 acgctactca aagcagggtt aacgctttct gaagggctgg ctctgctggc ggaacagcat
    41641 cccagtaagc aatggcaagc gttgctgcaa tcgctggcgc acgatctcga acagggcatt
    41701 gctttttcca atgccttatt accctggtca gaggtatttc cgccgctcta tcaggcgatg
    41761 atccgcacgg gtgaactgac cggtaagctg gatgaatgct gctttgaact ggcgcgtcag
    41821 caaaaagccc agcgtcagtt gaccgacaaa gtgaaatcag cgttacgtta tcccatcatc
    41881 attttagcga tggcaatcat ggtggttgtg gcaatgctgc attttgttct gccggagttt
    41941 gccgctatct ataagacctt caacacccca ctaccggcac taacgcaggg gatcatgacg
    42001 ctggcagact ttagtggcga atggagctgg ctgctggtgt tgttcggctt tctgctggcg
    42061 atagccaata agttgctgat gcgccgaccg acctggctta tagtgcggca gaaattgctg
    42121 ttacgcatcc cgattatggg ttcactgatg cggggacaaa aactcacgca gatctttacg
    42181 attctggcgc tgacacaaag tgcaggcatt acttttttac agggcgtaga gagcgtcaga
    42241 gaaacaatgc gctgcccgta ctgggtgcaa cttctgacac aaatccagca cgatatcagt
    42301 aacggtcaac ccatctggct ggcgctaaaa aataccggtg agtttagccc gctctgtttg
    42361 caattagtga gaacaggaga ggcatccggc tctctggatc tcatgttaga caacctcgcc
    42421 catcatcatc gggaaaacac aatggcgctg gcggataacc tcgcagcctt actggaaccg
    42481 gcgttgctga tcataacggg aggaattatt ggtacgctgg tggtggcaat gtatctgcca
    42541 attttccatt taggcgatgc gatgagtggg atgggataac gctggcgtgt tgctccacgc
    42601 cagcgttggg agattacagg ttgttgaaga tgcggttttc ttgttcctgc acacgaataa
    42661 acgtggtgcg cttggtcagc tctttcaggc gtgaagcccc aacgtatgta caagctgaac
    42721 gcaggccgcc caaaatatct cgcgcggtat tttcaaccgg gcctcgcagc ggcagcttaa
    42781 cggttttacc ttctgctgcg cgatattccg caacgccgcc aacgtgacgt ttcatcgcag
    42841 actcggagct catgccgtag aacagcataa atttctcgcc gttctcctca acgatgcgac
    42901 cgccgctctc ttcgtggccc gccagcatgc cgccaagcat gacgaaatcg gcaccgccgc
    42961 caaaggcttt cgccacatcg cccggcgtgg tgcagccacc atcgctgacg atcattccgc
    43021 ccagaccgtg cgcagcatcg gcacattcga ttaccgcaga aagttgcgga taaccgacgc
    43081 ctgttttgac gcgagttgta caaacagaac ctgggccaat gccaacttta acgatatcgg
    43141 cacctgagag gataagctcc tcacacattt caccagtcac tacgttacca gcacaaatgg
    43201 ttttggtcgg ccacgcttca cgcgctttcg caacgaactg cacgaagtgt tcggaataac
    43261 cattcgccac gtcaatacaa acgaagttta atgccgggtt caggtcgaga atctgtttag
    43321 ttttttcgaa atccgcatca gacgtaccgg tagaaaccat cacatgtttc agcacatcag
    43381 cggaagaatt gttgataaac gcttgccact cttcgacaga atagtgttta tgcacagcag
    43441 tcaaaatatc aaaagaagcc agcgcagagg ccatagaaaa tgtgcctacg gtgtccatat
    43501 ttgcggcgat aatcggcacg ccggaccagc tctgacctga atgtttgaag gtgaattgac
    43561 gttccagttc aacatcggaa cggcttttaa gagtggagcg tttagggcgg atgagaacgt
    43621 ctttaaaacc taacttcaga tcttcttcaa tacgcatgtg cggattcctg gggttaatgg
    43681 ctataagggt aagagcgcaa ctccagtgac gctatcatac gcactaatca atgcggcgca
    43741 agactgcgaa attgtctttt tttacgctac aatcccataa atttacctgg taaatagtga
    43801 gcgaacgttt catcaatttc gctttcgtgc actcattttt aacgttttga taataatgat
    43861 gaattcccgg gaatataaat tatgaggtat atagttgcct taacgggagg cattggcagt
    43921 ggcaagagta ccgttgccaa tgcgtttgct gatctcggaa ttaacgtcat tgatgccgat
    43981 attattgcgc gtcaggtggt tgaaccaggt gcacctgcgc tacatgccat tgctgatcac
    44041 tttggcgcta acatgattgc tgctgatgga acattgcagc gccgggcctt gcgcgagcgg
    44101 atcttcgcca acccggaaga gaaaaactgg cttaacgccc tgctgcatcc gctgattcag
    44161 caagagacgc aacaccagat ccagcaagct acttccccct atgtactgtg ggttgtgcca
    44221 ttgctggtag aaaactcact gtataaaaaa gcgaatcgag tgcttgtggt ggatgtcagc
    44281 ccagaaacgc aacttaagcg caccatgcag cgcgatgatg taactcgcga gcatgtcgaa
    44341 caaatccttg ctgctcaggc aacgcgcgaa gcccgccttg ccgtggcaga tgacgtcatt
    44401 gataataacg gcgcaccgga tgctatcgca tcggatgttg cccgcctgca cgcacactat
    44461 ttgcagcttg cgtcgcagtt tgtctcacag gaaaaaccgt aatgcagacc caggtccttt
    44521 ttgaacatcc actaaatgaa aaaatgcgta catggctgcg cattgagttt ttgattcagc
    44581 aactcaccgt taatttaccc atcgttgacc acgctggcgc gctgcatttc ttccgtaatg
    44641 tcagtgaatt actggatgtt ttcgagcgcg gcgaagtccg cactgagctg ttgaaagaac
    44701 ttgaccggca gcaacgtaaa ctccagacct ggattggcgt gcctggcgtg gaccagagcc
    44761 gtattgaagc attaattcag cagttaaaag cggcggggag cgtattaatt tccgcgccgc
    44821 gtatcgggca atttctgcgt gaagatcgtt tgattgctct ggtgcgtcag cgactgagca
    44881 tcccaggcgg ctgttgcagc tttgatttac ctacattgca catttggctg catctacccc
    44941 aggcgcagcg cgacagccag gtagaaacct ggattgccag cctgaacccg ctcacccagg
    45001 cacttaccat ggtgctggat ttaattcgcc agtcggcccc cttccgtaaa caaaccagcc
    45061 tgaatggttt ttatcaggat aacggtggcg atgccgactt gctgcgcctg aatctgtcgc
    45121 tcgattcaca gctttatccg caaatttccg gacataagag ccgttttgcc attcgtttta
    45181 tgccgctgga cactgaaaac ggacaggtac cggaacgtct ggatttcgaa ctggcctgtt
    45241 gctaaggagt aaaaatgtca gaaactatta cggtgaattg cccaacctgc gggaaaacgg
    45301 tggtgtgggg tgaaatcagc ccgtttcggc cattttgctc caaacgttgt cagctgatcg
    45361 acctcggaga atgggctgct gaagaaaaac gaatccccag cagtggcgat ctttccgaaa
    45421 gcgatgactg gagcgaagaa ccaaagcagt gacatttgtc tgatgccgca cgtaggcctg
    45481 ataagacgcg gacagcgtcg catcaggcat cttgtgcaaa tgtcggatgc ggcgtgaacg
    45541 ccttatccga cctacaaaaa caaatttatc aatgagttag aaatcccttt aggcctgata
    45601 agacgcgcca gcgtcgcatc aggcatcgtg tgcgaatgtc ggatgcggcg aaaacgcctt
    45661 atctgaccta cagacgttta agcttcgcaa ttaccggttc attggctggc ggaaaatcat
    45721 cggcattaag accgaccagc gacatccact caccgggttg cccttcttta ccccacggct
    45781 ccccttccca gcgttcgacc agccaaaacc acagtgttat atgcctgtcc gggaattcat
    45841 attccagttt ttcaaatagc gaaaaatgtt ggggggtaat cccgacttct tcctgaagtt
    45901 cacgcaccac cgcctgttcc ggcgtttcac ccatttcaat tttaccgccg ggaaactcca
    45961 gtttattcgc catgtgcgca tctgctgcgc gacgcgttat aaagatttca ttgttctcgt
    46021 tgcgaataat acctaccgca atttgcagct ttttcattgt cttaaaccta taaaaaaggc
    46081 gcagaatcct gcgcctttta cttcaacagt tagcttttat tgcaggcggc catggcactg
    46141 cttgtatttt ttaccagaac cgcacgggca aggatcgtta cgtcctactt tgcgctctcc
    46201 ggtttgcgcc gccagtgcag ctgcggctgc agagtcgtca tcctgatggc taagctgctg
    46261 catttgcgct aaacgctcgg cttccatacg acgctgttgt tccagctcct caacctcttc
    46321 aggcatacgt acctgaactt tgctcagcgt actgataact tcatatttca acgactccag
    46381 catcgctgca aacatggaga acgattcacg tttgtattcc tgcttcggat ctttctgtgc
    46441 gtagccacgc aggtggatac cctgacgcag atagtccatc gctgccaggt gctctttcca
    46501 cagggagtca agcgtttgca gcatgacgcc tttctcgaag tgacgcatca tctcagcacc
    46561 aaccacttct tctttacgct gatacacttc gatggactgc gccagaatgc gctcacgcag
    46621 cgtctcttca tgcagttctg gttctttatc cagccactcg gcaattggca aatcgaggtc
    46681 gaaatcgttc ttcagacgtt cctgcagccc cggaatatcc cacatttctt ccagcgactg
    46741 tggtggaatg taggcatcaa tggtcgcttt gaacacatct tcacgaatgc tgttaatggt
    46801 ttcgctcaca tcgctgacat ccaacagttc gttacgctgg gagtaaatgg cgcgacgctg
    46861 atcgttagcc acgtcatcat attccagcag ttgcttacga atgtcgaagt tacggctttc
    46921 aactttacgc tgggcgttgg caatcgcttt agtcacccac gggtgttcaa tggcttcgcc
    46981 tggcttcata cccagtttac gcatcatgcc ggatactcgg tcggaagcaa aaatacgcat
    47041 cagcgcatct tccatcgaca ggtagaaacg ggaagaacca gcatccccct gacgaccaga
    47101 acgaccgcgc aactggttat cgatacgacg ggattcgtga cgctcggtac cgatgatatg
    47161 caggccacct gcttccagta ccgcatcgtg acgtacctgc cagtcggctt taattttttc
    47221 aatttgctct gcggtcggat tttccagcgc ggcaacttct gcctgccagc taccaccgag
    47281 cacaatatct gtaccacgac ccgccatatt ggtcgcgata gtcaccgcag ccggataacc
    47341 tgcctgagca acaatcgccg cttcgttggc gtggaatttg gcgttcagga cgttgtgctt
    47401 aataccggct ttggtcagtt cgtttgacac cagctccgat ttttcgatgg agatagtacc
    47461 caccagcacc ggctggcctt tcgcagtacg ttctttgata tcttcaatga tcgcctgaat
    47521 tttttccgct tcagtcatgt agaccaggtc cggcagatct ttacgaatca ttggacggtt
    47581 ggtcggaaca acgacggtat ccagcttgta gattgagcta aattcgaaag cttcggtatc
    47641 agcagtaccg gtcatccccg ccagtttttc atacagacgg aagtagttct ggaaggtgat
    47701 cgaagccagc gtttggtttt cgttctggat ctgcacacct tctttcgctt ccacagcctg
    47761 gtgcagacca tcggaccagc gacggccctg catggtacga ccggtgtgtt cgtcaacgat
    47821 gataacttca ccatctttaa cgatgtagtc gacgtcacgg gtaaacagcg catgagcgcg
    47881 cagcgccgcc gttacgtggt gcatcagcat gatgttggcc ggagagtaca gagactcccc
    47941 ttcatccatg atgccctctt tcaccagcag ttcttcaatc agcaccagac cacgttcggt
    48001 caggttcacc tggcgagatt tttcgtccac cgagaagtgg ccttcgccct ggaaggtttc
    48061 ggagtcttct ttttcctgac ggatcaggtg cggaataatt ttattcacgc gtttatacat
    48121 ttccgagctg tcttctgccg ggccggaaat gatcagcggt gtacgcgctt catcgatcag
    48181 gatggagtcc acttcgtcca ccagcgcata gtgcagttta cgctgtacac gttcttcagg
    48241 gctgaacgcc atgttgtcgc gcaggtagtc aaagccgtat tcgttgttcg taccgtaagt
    48301 gatgtcagct gcgtaagctt cgcgctttgc cggtgctggc atgcccggca ggttgatacc
    48361 gacagtcagg ccaaggaatt caaacagcgg acggttgttt tcggcgtcac gttgcgccag
    48421 gtagtcgttg acggtaacta cgtgcacgcc tttaccggtt agtgcgttca ggtaagcagg
    48481 cagcgttgcg gtcagggttt ttccttcacc ggtacgcatt tcggcgatgc agcgttcgtt
    48541 aagaaccata ccgccgagta actgaacgtc gaagtgacgc ataccaaaga cgcgcttact
    48601 tgcctcacgt accacggcga aagcttccgg gatcagattt tccagcactt cgcctttttc
    48661 cagacgtgca cgaaactctg cggttttccc tttcagttct tcgtcggaga gtttttccat
    48721 ctccggttcc atggcattga tgatgttgac cactttgcgc atccggcgca gggtgcgatc
    48781 gttacgacta ccgaaaactt tagttaacaa tttgattagc ataataaaat ctcaaacgcc
    48841 ccgcgttgcg gagttaataa aatgaagtaa aggtttattg ttgttaggtg aggcgttgag
    48901 ggccagcacg gatgccttgc gcctggctta tccagacggg cgtgctgaat tttgcttgtg
    48961 gggtaaaatg cgcataatca atgcgataac ccttttcaga cggcgtgcct tcctgggtca
    49021 gcagcgcgct gagcgtatcc agtaatgcaa gatgttgcgc ctgaagaggc aaagattctt
    49081 cagcaacggg cagtgtttgc ggtgccattg cgaaagaaag atgacggatt accgtgcgaa
    49141 tggcatgttg atgccagtaa tcaacggaat agttcgaatt cgggcggcgt gtgttcgctt
    49201 ccagcaaggc caattgacca aagttaactt tggctgaagg ctcgtggttg cgggttgtcg
    49261 cttttgcggg cgcgtttggt tcggcggcgt tgctgagcgc aggcaaacct aaactcgccg
    49321 caaccatccc taataagaga tgcggccaga agtagcgttt accaaactgt cgccagcgcg
    49381 tcagtattcc actcacgtta ttgccatccc gatcatattc aaacgtattt agcgcacaaa
    49441 tcttatcatt aaagcctgca gtccacagca cgaatgaaga taagttgtgc ggaaaagtgc
    49501 ttaagaaaac agcgttcgca ccgtttttta tagctcttat aaaattcaaa aaaaaacgac
    49561 tggttcacct ggccggagag agtgccagat ttgccagtcg aattttatac gacagtataa
    49621 atgtcgttat gccagtacag ctgaaggcgc tttgaaggcc aacggcagtt ctgcgtcgtc
    49681 ctggaaggtc acatattccc aggcttcctg tttcgccagg acagcctgca gcagtttgtt
    49741 attcagtgca tgaccggatt tataagcggt aaatgcacca ataatattgt gaccacacat
    49801 gaacaagtca ccgatcgcat cgagcatttt gtgacgcaca aattcgtctt caaaacgcag
    49861 gccgtcttcg ttcagtacgc gataatcgtc aacaacgatg gcacaatcga agctgccgcc
    49921 caggcacaaa ccacgggact gcagatattc gatatcacgc atgaaaccga acgtacgcgc
    49981 acggctgatc tggcgcataa acgcatcagc ggagaagttc atcgcatagc gctggttgct
    50041 ggaatcaata gccggatggt taaaatcgat ggtgaaatcc agcgaaaaac cattgtacgg
    50101 cttaaattca gcccacttat cgccatcttc gacacgaaca gtctctttga tgcgaacaaa
    50161 ttttttggcg cagttcaact cgtcgatacc ggcgtcaagc agcaggtata caaacggagc
    50221 ggcgctgccg tccatgatcg ggatttccgg cgcgttaact tcgataacaa tgttatcgat
    50281 gcccaagccc gcgagagcag cattgaggtg ctctacggtt gaaatccgta catcatgctc
    50341 gttgaccaga cacgtacaga gcatggtatc acgcacagat ttggcatcgg ccgggaaatc
    50401 taccggtgga ttcaagtcgg tgcgacgata gatgaccccg gtgttggccg gcgcagggcg
    50461 taacgtcagg gtgactttct tgccggtatg taaaccgaca cccgtcgcct gaacgatacg
    50521 tttaagtgtc ctttgtttga tcatcgtatt atctcgccaa attacctatc caaccgaagt
    50581 gtactataca ttcggcgggc cagtttagca caaagagcct cgaaacccaa attccagtca
    50641 attcttaatc agcttgctta cgcaggaatg ctgggatatc cagataatcc ggctctttcg
    50701 cagtttgcgg cgcattgtca ttcacgactt tagcaaccgg cttctgctcc tgggtcagcg
    50761 gagccatccc atgctgctgg tagcgatcca tcactggctg ctgaacctgc ttattggtca
    50821 ccagagtgat ttcaggacgt ttgtccatgc cgatacctgt cgcaacaacg gttacgcgca
    50881 gctcgtcatt catatccggg tcaagagaag taccgataac cacagtcgcg ttgtcggaag
    50941 caaatgcacg gatggtgtta cctaccgttt cgaactcatc cagacgcagg tcgaagcccg
    51001 ccgtgatgtt aaccagcacg ccgcgcgcgc cagacaggtc gatatcttcc agcagcggag
    51061 aagagatagc catttcagca gcttcttccg cacggtcttc accgctcgcc acgccagaac
    51121 ccatcattgc gtagcccatc tcagacatta cggtgcgtac gtctgcaaag tccacgttca
    51181 tcaaacccgg acgagtaatc agttcagcga taccttgcac agcgcctttc agtacatcgt
    51241 tcgctgcgcc aaacgcatcc agcagggaga taccgcggcc cagaactttc agcagtttgt
    51301 cgttcgggat agtgatcaga gagtccacat gcttggacag ttcagtgatc ccctgctccg
    51361 cgaatgccat acgcttcttg ccttcaaagt tgaaaggctt agtgacgaca gcaacggtca
    51421 ggatacccaa atcttttgcc acttcagcga cgactggtgc tgcacctgta ccggtaccac
    51481 cacccatacc cgcagcaata aagaccatgt ctgcaccttc cagcgccgca cgcaatgcat
    51541 cgcgatcctc atcagccgca ttgcggccaa cttctggatt agcgccagcg cccagtcctt
    51601 tggtgatacc gctaccgatt tgaatcgtct gtccaaccgc tgttttacgc agcgcttgtg
    51661 catcggtatt taccgcgaag aattcaacac cttcaatgcg ctcgcgcacc atgtgttcaa
    51721 cagcattacc gccgccgccg ccgacgccga tgactttaat caccgcgtca ttggtaagtt
    51781 ccattggttc aaacatagtt tctctccgat ttgtgcctgt cgcctgaggc cgtaatcatc
    51841 gtcggcctca taaaaattaa aactcttttc gcagccaact attgagtcgc ttgatccacg
    51901 agccaactga tgctgtaaca cgtttttcta cttcagcttc accgttaaga tgtgactctt
    51961 tcccatagtg aagcaatccc accgccgtcg aataatacgg ctcctgagca taatccgtta
    52021 aaccggtaat gttcagcggc gcgccgatac gcacttgcgt atgaaacacg cgctgagcac
    52081 aggctgcaag accttcgatc tgcgctgcgc caccggttaa tacaatgcct gccgccaggt
    52141 gatgtttaac cccttgttgg cgaagctttt cctgcaactg caatatctct tcgttgacca
    52201 ggttgagcag ctcggtatag cgcggctcga tcacctctgc cagtgtctga cgttgcagac
    52261 tccgtggcgg acgaccacct acgctcggca cttccacgct ctcatctttt ccaacgatgg
    52321 aacccagcgc acaaccgtgg cgaactttaa tcgcttcggc gtcgcttggc ggcgtgccaa
    52381 aggcgtaagc gatatcactg gtcacgacat tgccagcata aggaattacc ttagtgtggc
    52441 gcaatgcccc accggtataa acggcgatat ccattgtacc accaccgata tcgacgacgc
    52501 agacacccag ttcacgttca tcttccgtca ataccgaata acttgatgcc agtccggcaa
    52561 atatcagttg gtcaactttc agcccacaac gttcaaccgc tttgacgatg tttttcgcca
    52621 tatcgttgtg acatgtgatc aggtgcactt ttgcctgcat ccgcacgccc gaaagtccta
    52681 ccggattctt gatcccttcc tgatagtcaa tcgcatactc ttgcgggatc acatgcagca
    52741 cacgatgctc atcgcgcaca cgcaccgatt tcgcggtatg gacgacgttt tccacatctt
    52801 cttgcgtcac ttcttcttca gaaataggca ccataccaat ttcattctgg cagctgatgt
    52861 gcttaccaga aagcgccaga tataccgaag agatctgaca atctgccatc aattctgcct
    52921 ggtcaatggc gcgttgtacg cacttgacca cggattcgag gtcgttcacc ccgcctttat
    52981 ccataccacg cgacgggcag ctgcccacgc caatgatatt gaccataccg tcgggcagaa
    53041 cttcccctac taaagcggca accttcgcgg taccaatctc cagtcctact accagttttc
    53101 tgtccgtcgc cttgatcatt gttgttctgc ctgtgcctga ttttgttgct gagtagattc
    53161 ctctggcggc aagggcgccc agcctactgc cgctccagag tcataacgca aatcaacgta
    53221 gctaatccgt ttgccatcgg tttgcgcctg ctgctgtaaa accggataaa gttctacaaa
    53281 gcgagccaaa cgtttcatcg tatcgccccg gccaagattg agcttaatat cgttattcag
    53341 cgtcaactgc caggaacgcc gcgcggtcat cgccgcttcc ttcagagtaa atctgtcctt
    53401 tgccagcatc tgccccattt cgcgatagcc ctgcaacact tcattggcgc tgccttccgg
    53461 accatacagc attggaagca cctgcttgct ggtgcgttct ggcggcacgc tgaaggtatt
    53521 tccttccgcg tctaccatat gttgatcatt ccaccgcgca atcggcacat attcaaccag
    53581 atgaatcttc aattcatcag gccactgctt tctgacgctc acctgcttaa tccacggcag
    53641 gcgttgttct atttgcgtct ggatgatgtt gacatcctgg gtcataaagg tacccggctc
    53701 acccaatgcc aggatcgact gccggatatc gtcattacgt gtgtaatggc gttcaccggt
    53761 caacaccagc tttgagagcg gcaggcgttg cgcatcttcc atccagccca acacgaccca
    53821 gccgctcacc aacactgtcg ttaaaacggt cagcaggaaa aggatccccg ccagacgcgt
    53881 tccattattg cggcgagaag aaacctcttc ttcgctgttt cgcgtgttca gagcagcctg
    53941 cgacatatta gtccgccagt tccagaattc gtactaccaa ctgcgagaag ctcatacctg
    54001 cctgacgtgc cgccatcggc accaggctgt ggctggtcat acccggtgag gtattggctt
    54061 ccagcagata aaactgtcca tcgctgtcca gcataacgtc aatacgtccc catcctttgc
    54121 aacctaacgt cgtccatgct ttcagcacta atgcctgcaa attggcctct tgtgacgctt
    54181 ccagacctgc ggggcagaaa tactgtgtct catcagagag atacttcgcc tcataatcat
    54241 agaaggttcc ggacggttga atacgtattg acggtaaaat ttcttcaccg agtatcgcaa
    54301 ccgtgaactc cggcccactt agccattttt caatcaatac ttcttcatcg tgctgaaatg
    54361 ccaatcttaa tgcatcttgt agagcatttt ctgctactac ttttgacatt cccacactgg
    54421 aaccttcgcg gctcggctta acgataaccg gcaaacccag agcagaaatt tctgctaact
    54481 gcttatcgct caggcctttt tcaaactctg cgcgggttaa cgctacccac ggcgcgaccg
    54541 gtaaaccggc accttgccat agaagtttgc tgcgtagttt atccattgaa agcgcagatg
    54601 ccatcactcc gcttccggta taaggcaagc ccatcagctc gagcatcccc tgcagcgtac
    54661 catcttcacc gccgcgaccg tgtagcgcga taaacacttt ctgaaagccc atcgacttca
    54721 gttgcgtcac gtcgacttct ttcgggtcga caggatacgc gtcaataccg ccttcacgca
    54781 gtccggctaa caccgctgcg ccagaattca gagaaacttc ccgctcagcg gaggtcccac
    54841 ccaacaggac cgcgatttta tcagtcatgt tgttcttcct ccggagtttg cggcttcagt
    54901 ttgatttcag ctaaagaacg ggcaattttt ccaatattac cagccccctg aacgagaatc
    54961 aggtcgttac cggttaatac cggtgccagc atctcggcta cccgcgccgg atccggcacc
    55021 agaatgggat caattttccc acgtccacga attgtgcgac acagcgaacg gctgtccgct
    55081 cccggaattg gcgcttcgcc agccggatac acttccagca tcaacagggt atcaacctgc
    55141 gtcagcacat tggcgaaatc atcatacagg tcgcgcgtac gggtaaaacg gtgcggctga
    55201 aacagcatta ccaggttttt atccggccag cctgcgcgcg ccgctttaat ggtggcgtcc
    55261 acttccgtcg ggtggtggcc gtagtcatcg accagcattg ccgtaccgct tttaccattc
    55321 actggctcca gcgggaattc accgaggaaa tcaaaacggc gaccagtccc ctggaagctt
    55381 tcaagcgccc gcaaaatagc ctcgtcgtca atgccctctt ccgtagcaac cgcaaccgca
    55441 gctgcggcgt tcagcgcgtt atgacgacct ggcgcattca gggtgacgcg catcggctct
    55501 ttgtcctggc gcagcagcgt aaagtgcccc tgcgggccaa tctgctgata atcttctaca
    55561 cgcacgtcgg catcttcgct gaagccgtaa gtcgtggtct gacgccccac tcgcggtaac
    55621 aattcgcgga tcaccggatc atcaacacac atcaccgcac gaccgtaaaa cggcaggttg
    55681 tgcagaaaat taataaaagt ctgttttaaa ttctcaaagt cgccctggta ggtatccatg
    55741 tggtcggctt cgatattggt gacaatcgcc accatcggtt gcagatgcag gaacgatgca
    55801 tcactctcat ctgcttcggc aatcaggtac cgaccatgcc ccaaacgcgc atgaaccccc
    55861 gccgctttta ccagcccgcc gttaacgaag gttgggtcga gccccgcttc tgcgtagatg
    55921 ctggaaacca tcgcggtggt tgtcgttttg ccgtgcgttc cggcaatggc gatgccatga
    55981 cgaaaacgca ttaactcagc cagcatttcg gcacgacgga tcaccggaat acgcgcttca
    56041 tgagcggcga caatttccgg gttatcggca gaaatcgcgc tggaaacaac gaccacgctg
    56101 gcatcacgta cgttttccgg gcgatggttg aaataaatcg tcgcacccag attcattaac
    56161 tgctgcgtga ccggatttgg cgctaaatcg gaaccactga tctgataacc ttcattggcc
    56221 agaacttcgg caataccgcc cataccggca ccaccaatgc cgacaaaatg tatgtgccga
    56281 acgcgacgca tttcgggcac gatggaacgc agttttgcca attgttgtgt attcattctt
    56341 tacgccatta acttcttaaa ttcatacgat gcaaaaggca tcgctacaat tacgcccggg
    56401 caacccggct cacttcattt gccactcgct cggtggcatc cggaatggat gcagcgcggg
    56461 cgcgttctgc catggttaat aaggtttctc gcgaccaccc ggccagggtg ttggcgacag
    56521 catccacgct aagctgtggc tgctcgataa ttttggctgc gcccgctttt tccagcggta
    56581 gcgcattcca gtattgctgg cggtctttat gttgaaacgg cacaaacaac gccggtagtc
    56641 ctgccgcggc gatttcactc accgttaacg caccggagcg gcaaacgacg acatccgccc
    56701 acgcatacgc cgccgccata tcatcaataa attccgtcac tttatgctgc ggttgccccg
    56761 cttcggcata cgcctgttca acggattgtt gcgaaccttt gccgctctga tgccagatag
    56821 tgactgaatc acccagtttc gcagcaacct gcggcattgt ctggttaaga atgcgtgcgc
    56881 cctgagaacc acccactacc agcacacgaa ccggaccttc acgtccagcc aaacgttgct
    56941 gcggcaacgg cagcgccaac acatcggtac gcaccgggtt acccactact tccgcattag
    57001 ggaaagcacc tggaaacgcc tgcatcactt tggtggcaat cttcgccagc catttattgg
    57061 ttaagcccgc aataccgttt tgttcatgaa gtacaaccgg aatgcctaac gaccacgcgg
    57121 ccagaccacc tggacctgac acgtagcctc ccataccgag caccacgtca ggtttgtacg
    57181 ctttcataat cgcccgcgcc tgacgccagg cgttgaagat acgcagcggg gcagctatca
    57241 gtgcttttat accttttcca cgcagaccag agatacgaat gaaatcaatt tcgatgccat
    57301 gttttggcac taagtccgct tccatacggt cggcagtccc cagccagcga acttgccaac
    57361 cctgagccat tagatggtgc gcaaccgcca gtcccgggaa tacatgtcca ccggttccgc
    57421 ctgccatcac cattaatcgc tttccttgac cactcatcgt gaacctcgta caaacgcctg
    57481 cgctttctcc agacgcgttt cataatcaat acgcaacagc atcatgatgg ctgtcgacat
    57541 aatcagtaag ctcgaaccac cgtaactgat cagcggcaat gtcagacctt tggtcggtaa
    57601 catccccgcc gccgcgccta cgttaaccag cgcctggaag ctaaaccaga tgccaataga
    57661 acaggcgaga aaaccggaaa aacggtggtc aatttctaat gctttacggc caatcgacat
    57721 cgcgcgaaaa gcgacgaaga ataccattaa aagtgccagc accacaccga cataccccag
    57781 ttcttcgccg ataatggcga aaataaagtc agtgtgcgct tccggcagat actccagttt
    57841 ttgtaccgag ttacctaaac cttgccccca aagttcgccg cgaccaaacg ccatcagcga
    57901 ttgcgttaac tgatagccgc tgccaaaggg atcttcccac gggttccaga atgcggtaac
    57961 acggcggata cggtacggtt cggcgagtat cagcaacaca accgctgaaa tgcccatacc
    58021 gataatggca atgaactgcc acaatttcgc tcccgccagg aacaacatcg ccagcgtagt
    58081 cacaaacaac accaccaccg taccaaggtc tggctgtgcc agcagtaaca ctgccaacac
    58141 cagaatcacg cccatcggtt tcaggaagcc gcgcaggtta ttacgtactt cgtcgccttt
    58201 acgcaccaga tagttggcga tatagcaaaa cagcgacagt tttgtcagct ccgcaggctg
    58261 gatacgcagc aaaccgagat cgatccaacg cgatgcccct ttaaccgagc tacccactac
    58321 caggacgatc atcagcagga tgatagatcc gagcagcatc gtggcactgt agcgttgcca
    58381 gaactccatc ggcagacgca gcgtaatgat cgccagaata aacgccaaaa tcagatagac
    58441 accatcacgc ttcgcgaaga agaacggatc gttggttaag cgttgcccta tgggcattga
    58501 cgccgaggtc accatgataa agccaatcgc cgcgaggccg aaggtcagcc acagtaaggt
    58561 gcgatcgtac atgatcaggc tgtcggtatc tttttcccgc gagcccatca cccagccctt
    58621 tagcgccgtg gagatccaga ccaggatact gaatcctggc aggcgcggca ttttcaggcg
    58681 agggagagat aaacgcatca acctaactcc ttcgccagac gggcaaactc attgcctcgt
    58741 tgttcaaagt tcttgaactg atcaaggctg gcacaggctg gggagagcag aaccatatcg
    58801 cccggctgaa cacgcggagc cagcaagcgc atcgcctgtt ccatagtttc ggtttgttct
    58861 gccacttccg ggcgtagcgc cgccagctgc gcgccgtcac gaccgaaaca atacagacgt
    58921 acgttatcgc cattcaggta acgcgccagt gggctaaagt ccgccgattt accatcgcca
    58981 cccagcaaca aatgcagtgt gccgtctacg tgcaggccat tcagcgccgc ttccgtactg
    59041 ccgacgttgg tcgctttcga atcgttaatc caacgtacgc cgttatgctc cagcacaact
    59101 tcaaagcgat gcggcagacc agtgaatgtg gttaacgctt tcaggctgct ggcacgcggt
    59161 aaccctgcag catctgccag cgccagcgcc gccagcgcat tggtgtagtt atgctgcccg
    59221 gaaagtttca tctctttcac attcagcact ttctcgcctt taacccgcag ccaggtttcg
    59281 ccctgctgat gattcaggtg atagtcaccc atgttgacgc caaagctgac gcagcgttca
    59341 tccgcaccgc gaatcggcat tgttaaggca tcatcagcat taaccacgca aactttcgcg
    59401 ttttcgtaaa tgcgcagttt tgctgcacga tactgttgta aaccaaacgg atagcgatcc
    59461 atatgatctt cagtcacgtt cagaatggtc gctgctaccg cctgtaagct ggaggtggtt
    59521 tccagctgga agctcgacag ttccagcacg tacagttcac actcatcatc cagtagcatc
    59581 aacgcaggca ggccaatatt gccacccaca ccaacgttaa cccccgccgc tttcgccatt
    59641 tcacccacta gcgtggtgac cgtgcttttg ccgttagaac cggtaatcgc cacaatcggt
    59701 gcttgtgctt cgcgacagaa cagctcgata tcgccaacga tttcgattcc ggcatcagcg
    59761 gcagcgctta aggatggatg cgccagtgca ataccgggac tggcgacaat cagatctgcc
    59821 gccatcagcc attcatcatt cagactgccc gtgtggcgtt ctacggcttc gggtaattta
    59881 tccaggccag gcggtgtcat acgcgtatcc ataacgcgcg gcgtcacacc gcgagcgagg
    59941 aaaaagtcca cgcaggaaag cccggtgagg cccaggccga taatgacgac atttttaccc
    60001 tgataatcag ccatgattaa cgtaccttca gcgttgccag accaatcaga accagcatca
    60061 gcgaaataat ccagaaacgc acaatgacgc gcggttccgg ccagcctttc agttcatagt
    60121 ggtgatgaat cggtgccatg cggaaaatac gttgtccgcg cagtttaaag gagccgacct
    60181 gcaggatgac agaaagcgtt tctaccacga acacgccccc cataatcacc agcaggaatt
    60241 cctgacgtag cagtacggcg ataatgccta acgcaccacc taacgccagc gaacctacat
    60301 cgcccataaa gacctgcgcc ggataggtgt taaaccacag gaagcccagt cctgccccga
    60361 ctatcgcggt acagacaata accagttccc cggcgtgtcg cagatacggt atatgcaagt
    60421 agctggcaaa gttcatattg ccggtcgccc acgccaccag cgcaaaacca ccggcgacaa
    60481 atacggtcgg cataattgcc aggccgtcga gaccatcggt caggtttacc gcgttgccag
    60541 tacccacaat gacgaagtaa gccagcagaa tgtagaacag ccccagctgc ggcatcacat
    60601 ctttaaagaa tgggaccacc agctgcgttg cgggcgtgtc tttgccggca aggtacaggg
    60661 cgaaggcgac acccagcgca atgaccgaca tccagaaata cttccaacga gcgatcaacc
    60721 ctttggtgtc tttacgcacc actttgcgat aatcatcaac aaagccaata acaccgtaac
    60781 ctaccagcac caccaacacg caccagacgt acggattgga cgggtaagcc cacagcagta
    60841 cggagatcac aatcgccgtc aggatcataa tcccgcccat ggtcggcgta ccgcgcttgc
    60901 tgaagtgtga ttcaggaccg tcgttacgca ccacctgacc aaaggaaagt ttttgcaaat
    60961 gagcaatcat acgcgggccc atccacaatg agatgaacag cgcggtcagc aggctgacga
    61021 tggcgcgaaa cgtcagatag gaaaagacgt taaagccgga ataatatttg accaaatgtt
    61081 cggccagcca aactaacatg tcccattctc ctgtaaagcg cgtactacct cttccatggc
    61141 ggcactacgt gaacccttaa ctaaaatcgt aattacctgt tgctcagcaa tcagtaattt
    61201 aagacgcgta attaacgcag ttttatcagc aaaatgttcg ccaacgccgc tggcggtgct
    61261 gatagcatgg ctttgtttac ccacgcttaa cacgcggtca ataccagcag cttttgccgc
    61321 ctcgcccacc tgtacatggc aggcttcgct ttcagcgccc agttccgcca tatcgcccac
    61381 caccagcacg cggtagcccg gcatttcagc cagtacctgg actgctgcag tcattgaacc
    61441 gacattggcg ttgtaggagt cgtcgagcag caactggttt tctgccagtt ggatggggaa
    61501 cagacggcct gaaacagctt tcagatttgc cagccccgct ttgatagcat caagcgttgc
    61561 gcccacggac atggagagcg cagcggctgc cagcgcattc gcaatattgt gacgccccgg
    61621 caacggcagc agaacatcga cgctaccggt tggggtttgt agggtaaatt ccgtaccgtg
    61681 cgaggtcaca tggatattgg tggcggtgaa atcgctgttg gcggcattgg gtgagaaacg
    61741 ccacactttg cgtgagccaa ttacgctctg ccagttcagc cagtcgttgt tgtcggcgtt
    61801 cataatggcg ataccgtttt ccggcaggcc gctaaagatt tcacctttcg ctttcgcgac
    61861 acccgcaagc gagccaaaac cttccagatg cgccgctgcc aggttgttga ccagcgcagc
    61921 ttccgggcga gtcagactca cagtccaggc tatttcgccc tgatggttcg cgccaagttc
    61981 aataactgcg taatcgtatt ccggcgttaa gcgcaacagc gtcatcggta caccgatgtc
    62041 gttgttgaga ttgcctgccg tataaagcgt gttgccgcac tggcttaaaa tcgccgccgt
    62101 catctcttta acggaggttt tgccggagga ccccgtcaga gcaaccacgc gcgccggaac
    62161 ttgctggcga acccatgcag ccagttcacc aaacgccaga cgcgtatcct tgacgattaa
    62221 ctgcggcagg tcgatgtcca gcggacggct aaccagtagt gcgcctgcgc cgccagcttt
    62281 cgcctggtcg gcaaaatcgt gggcatcaaa acgttcgcct ttcagggcaa caaacaggca
    62341 gcccggcgtc agttttcggg tatcagtggt tacagcatca agggtgatat ctgcaccttg
    62401 cagttcaccg ttgagaatgt cggtaagttg gctaagggtt acgctaatca tgcaatcacc
    62461 cccagcagac gcgccaccgt gacgcgatcg gagtagtcca gacgctgatt gccaacaatc
    62521 tggtaatctt catggccttt gcccgcgacc agtaccacat cattctcttt agcctgcata
    62581 acggcgcaag tcaccgcttc agcacggcct tccatcactt tggcatgtcc ggcatctaac
    62641 attcccgcca gaatatcgtt gatgatggca cgcggttctt cggtacgcgg gttatcgtcc
    62701 gtcaccaccg ccacgtcagc aaactcttcg gcaattgcgc ccatcagtgg acgcttacct
    62761 ttatcgcgat cgccaccaca gccaaagaca caccacagct tgcccgcaca gtgcagacgc
    62821 gccgcctgta aggctttttc cagtgcatcc ggcgtatgcg cgtaatccac caccaccgtc
    62881 ggtttgcctg gcgcagtgaa cacttccata cgtccgcaaa ccggttgcag acgcgcggcg
    62941 gttttcagca gatcagccag tggatagccg agtgccaaca gtgtcgccag cgcgagcagc
    63001 aggttgctga cgttaaaagc gcccatcaga tggctttcaa tttcgccatc gccccaactt
    63061 gagctaaagc gaatcgtcgc accgctgtcg tgatagttca cttcggtcgc tttcaaccag
    63121 cgtccgtgac agttcggatt aatatgatct tccattgata ccgcaaccgc gtccggcagt
    63181 tttgccagcc agcggcggcc cacttcatcg tcggcgttaa taatcgcctg accgcaatga
    63241 tgctcagaat aaagcagcca tttcgcggct tcgtagtgtt ccatatcacc atgataatca
    63301 aggtgatcgc ggcttaagtt ggtaaagacc gacgccgcaa atttcaatgc cgccacacgg
    63361 tgctgtacca gcccgtggga ggaaacttcc attgcgcaaa acgtcgcgcc ctgatccacc
    63421 agccccgcca gctcatgctg aacatcgact gccgaaccgg ttgtattttc tgtcgggatc
    63481 actttcccca gcaggccgtt accaacggtg cccattaccg cgctggtttc gccaagcagt
    63541 tggctccact gcgccaacag ctgggtagtc gtggttttgc cgttggtgcc cgttacgccc
    63601 acgagacgta aattgtcaga gggttcatgg taaaagcggc ccgccagtgc agataaacgc
    63661 tcgttgagct ggctgagata gatgaccggt acgccgtgca tttcacggat ttcaccatcg
    63721 gtcgcctcat ctttcgcctc tgcaataatg gcagccacac cttgcgctat cgcctgcggg
    63781 atatatcgac gcccgtccgc ctgatgacct actacagcta caaagagatc gcccgccgca
    63841 gccacacggc tgtcgagtgt catctctcgc agtgctcgcg aaggtgcgtc tggcacccac
    63901 ggagcaagaa ggtcgcgcaa attacgatct gccacctgtc ccctcgcctt gattaatcac
    63961 aaattcattt ttatcgcccg ttgtcagcgc atccggctcg atgttcatgg tacgcaatac
    64021 gccgcccatg atggcaccaa agaccggcgc ggaaacggcg ccgccgtagt atttacccgc
    64081 ctgcggatcg ttgataacaa caaccagcgc gaagcgcggc tgactcgcag gcgcaacgcc
    64141 tgcggtataa gcaatatatt tattgatgta gcgaccgtcc ggcccgacct ttttcgcggt
    64201 accggtttta atggcgatac gatagccttt aatcgccgcc ttcacgccgc cgccgcctgg
    64261 tagcgccacg ctttccatca tatgcaccac agtgcggaca atggattccg ggaagacacg
    64321 ttcaccggga accggggggt caactttggt aatcgacagt gggcgataaa tgccgtagct
    64381 gccgatagtt gcgtagactc gcgctaactg taatggtgtt accattagcc cgtagccgaa
    64441 agagaaggtg gccctctcta tgtcagacca ccgttgtttt tgaggatata agccactgcg
    64501 ttctccgacc aaccccaaat tggtcgcttt tcccagtcca aaacgtgagt aagtatctac
    64561 taacgctgag gacggcatcg ctaacgccag cttggaaaca ccgacgttac tcgacttctg
    64621 taataccccg gtcagggtta attcgctgta gcgtgccacg tctttgattt cgtggccgtt
    64681 aattcgataa ggaatggtat tgagtaccga gttttcccgc accacgccac gttgcaacgc
    64741 ggtcattacc accatcggtt taaccgttga gcccggttca aacacgtcgg tgatggtacg
    64801 gttacgcatc gcctctttcg gcgtgccgct cagattgtta gggttgtatg acgggctgtt
    64861 agccatcgcc agcacttcac cggtgttgac atccaccagc acggcgctac cagattcagc
    64921 cttgttaaag gccaccgcgt tgttcagttc gcgataaacc agcgcctgca ggcgttcatc
    64981 aatactcagc gccaggttgt gcgctgcctg gctgtcagta gaagaaatat cttcaattac
    65041 gcgaccatag cggtctttac gcacaatgcg ctcacccggc tgcccggtaa gccatttatc
    65101 gaaactcttc tcaacgccct caatcccttg actatcgacg ttagtaaagc cgatgaggtg
    65161 agcagtcact tcgccggacg gatagtaacg gcgagactct tcacgcagat gaatccccgg
    65221 cagtttcagt tttttgatgt agtccgccat gtcagggttc acctgacgcg ccagataaat
    65281 aaagcgccct ttcgggttgg cgttaatgcg ggctgaaagc tgatccagcg gaatattgag
    65341 cgcgttagcc agcgccttcc agcggtcacc gacgctgata ccgccagcgt catgcacttc
    65401 tttcgggtca gcccaaatcg cttttaccgg cacgctcacc gctaacgggc gaccagaacg
    65461 gtcagtaatc atgccgcggg aggtggaaac ttgctgaacg cgaagagaac gcatgtcgcc
    65521 ctctttcacc agcatatccg gggagataac ttgtaaccac gctacgcgtc cgagcagaaa
    65581 agccagcgcc aggagaatac agccgcataa caacgcaaaa cgccaactga taaagttggc
    65641 atgttcttcc tgacgttttg gtttctgcgt tttcgccgct gctttcatgc gtcgcgttta
    65701 tccttatttt tgcactacga tattttcttg tgacggatca acatgctgca tttgcagctt
    65761 ttccgtggcg atcctttcca cccggctatg gtcgccgagc gcattctctt caaggatcag
    65821 gttgcgccat tcaatgtcta aagcatctcg ctccagcacc agttgttcgc gctgagcggt
    65881 cagtaaacgg gtatggtgcg ccgtggttac cacagtcacc gccgtcaaaa taatgcaaat
    65941 gaacaggcag agtggcagct tcccaaatcg caaaagatcg tcaccgataa caccaggcaa
    66001 tgcatggcgc tcgtggcttc ccatcgatcc tttaactttg cttagagcgt ctgtcactct
    66061 gctgatcatg cattcgtcct ctctgcaata cgcagaactg aactacgggc acgagggttc
    66121 tcagccacct cttcttcgcc cggcattaac ttgcctagtg ctcgcagctg acggccaccc
    66181 agttttttga gctgctcttc agtcatcggt aaccctgccg gaacttgcgg accgcggctg
    66241 ttttcacgca taaaacgttt cacaatacgg tcttccagcg agtggaagct gatgatcgaa
    66301 agccgcccac ccggggccag cacgttgagc gagcttttta gcgcctgctc tatctcctcc
    66361 agttcactgt ttacccaaat gcgcaccgcc tggaaggtac gggtcgcggg atgtttaaac
    66421 ttatctttca ccggcgttgc agcagccacg acttccgcca gttctttggt gcgggtcatc
    66481 ggctgttcgc ggttacgctc gacaatggcg cgggcaatgc gtttggcaaa acgctcttca
    66541 ccataggttt tcaataccca ggcgatatcg gcttcttctg cggtttgtag ccattcagcg
    66601 gctgactgcc cacgggttgg gtccatacgc atgtccagcg gaccatcgcg cataaaggaa
    66661 aagccacgtt cagcatcatc aagttgcggt gaagagacgc caagatcgag gagaatgccg
    66721 tcgatcttgc cgataagatc gcgctcggca acgtattcgc ccagcgcgga gaaaggtccg
    66781 tggatgatgg agaagcgcgg atcatcaata gtcttcgcaa cggcgatagc ctgcgggtcg
    66841 cgatcgatcg ccagcaaacg cccctcttcg ccaagctgcg agaggatcag acgtgagtga
    66901 ccaccgcgac caaaagtccc atcaatgtag atgccatcag gacggatatt gaggccatta
    66961 acggcttcat ccagcagcac cgtagtatgt ttatagtttt ccatcatttt atagagacaa
    67021 gtcctgcagt cgctccgata agtctccggt agccaactgc tctgcgtcga tatcttcctt
    67081 gacctgttga tgccaggttg tttcatccca cagctcaaac ttgttgaact gtccaaccag
    67141 catcacttct tttgtcagcc cggcatgttg ccgcagtact ggcgcgatta acaatcgacc
    67201 tgcgccatcc atctgacatt cgctggcatg acctaacagt aggcgctgca cacggcgctc
    67261 aaccgggttc atgctcgaca gacgcgataa tttttgctcg ataatttccc attcaggcag
    67321 ggggtaaagc agcaggcacg ggtgataaat gtcaatggtg caaaccattt gaccggcagc
    67381 gttctcaagc agctgttccc gataacgggt aggcactgat aagcgccctt tgctgtcgag
    67441 attgactaac gttgctcccc ggaacatgcc agcctcaccc cttattccca ctttgcccca
    67501 caatttccca ctgaaaggag tttacggagc tgaggaaaag cttgtcaagc cagtcacaag
    67561 gcataccgag gtaaaaaacc cgatgtttac ggctaatatc agcgttgttt attttacgtc
    67621 caacgtgcga agcaaattaa cgttatgaat agctgtaaga aaaaatccct catcgtcatc
    67681 accgttttaa aatgactcac tattttccac agcaaatata aagtgtcagt ttgcgacgcg
    67741 agcggcattt taggacatat cttccccggt taacagtcct tgttacgtct gtgtggcgct
    67801 tgatgccagc gggtttgcgc aaaagcaaag aaaagtgttt gttaaatcgg caattcatcg
    67861 ccgcatgtaa caaaataatg caaaaacgcg ctgtcgatat cacttaatta ttgtgaattt
    67921 cagctggtga gttaattaac gggcgggttt aaaagcattt atcaataata gtcaggaata
    67981 gtcttattta ctttaagcat attgatgtcc agtcccgtac tctacgcgcc agagggaaat
    68041 tcacctggcg cgtatttttg ttcgcggctt agctacggct gagcacgccg cggcgataga
    68101 gattacgttt aatgcgcgtt aaaccaggtt ttggcttacg cggttcgtcc aggcttgcca
    68161 ggacaatctc cagcacacgc tctgcgacat cgcggtgacg ttgagccact gccagcaccg
    68221 gacactgtaa gaagtcgagc agttcgttat cgccaaaggt ggcaattgcc aggtcagaag
    68281 gcagtttgcc gtcgcgacgc agcgtgacat ccatcactcc ttgcaacaac gcaaacgacg
    68341 ttgtgaacag cgcctgcggc atcggatgcg tttccagcca tttttcgaat aactgggcag
    68401 ccgcctcccg ctcatagctg ttggcataca ggaaatgcac ttcgcgcgga tcatctttcc
    68461 aggcagtacg gaaaccttgt tcacgcagga agctgacaga aagctccggt agcgcaccaa
    68521 gataaagcac cgtctcggcg ggaaacttac gtaactcttc cgccagcatt tcggcatcat
    68581 cctgatcggc accaaccacg ctggtgaagt gttcacgatc gagggcgcgg tccagcgcga
    68641 caatcgggaa cgggtcgtta gcccagcgtt gataaaaagg atgctcagga ggcaacgacg
    68701 tcgaaacaat aatggcatca acctgacgct gtaaaaggtg ctcaatgcac cgcatttcgt
    68761 tgtctggctg atcttctgag caggcaatca gcagttgata accccgttgc cgcgcctggc
    68821 gttcaagata gttagcgatg cgggtatagc tggtgttctc cagatcgggg atcacaagac
    68881 caatagaacg tgtgcgtcca gcacgaagcc cagctgccac ggcgttcggg tggtaattgt
    68941 gctcacgcac cacagccatg actttttcaa cggttttgtc gctcacacgg tattgcttcg
    69001 ctttgccgtt aataacatag cttgcagtgg tccgcgacac tcccgccagc cgagcgattt
    69061 catccagttt cacaattgcc ccttgcgtaa aatgtaaaaa ccatagccat tgtactggta
    69121 tgggttaaat tcattaacat ctaagcgcag aaaattaact gcggcaactg cttttattcc
    69181 aggctcccac tgatttcgca aaaaaagccc aacgtgacat gttgggctgt aaattgcgca
    69241 ttgagatcat tctcaacgca ttattttatc gccgcgcgaa agtccgacca caccagagcg
    69301 agcaacctcc acaattttcg ccacatcgcg aatcgatgct aaaaatgcat caagcttacc
    69361 gctggtgcct gctaattgaa cggtataaag cgagggtgtg acatcgataa tttgcccacg
    69421 gaatatttcc gtattacgtt tcacttcgtc acgcccgtaa ccgctggcct gaattttcac
    69481 caacatgatt tcccgctcaa catgcgcgcc ctgccccaac tcactcacgc gcaagacatc
    69541 gaccagtttg tgtaattgct tttcgatctg ctcaagtact ttttcatcgc ccacggtctg
    69601 gatggtcata cgcgataatg tcggatcgtc ggttggcgca acggtcaggc tttcaatgtt
    69661 gtagccacgc tgggaaaaaa ggccaatcac gcgggataac gcgcctgatt cattttcgag
    69721 taagactgat aatatccggc gcataatcag gttctctccg ttttgcttaa ccacatttca
    69781 tccattccgc ccccgcgaat ctgcatcggg tagacgtgct cgctgccatc gacggtaaca
    69841 tcaacaaaca ccaggcgatt attgcgcacc tgttccagcg cctcgctaag tttgctttcc
    69901 agctcatgcg gatgagaaat ctggatcccg acatgcccat aggcttccgc cagacggacg
    69961 aaatcgggta gcgattgcat ataagattgt gaatgacggc cggaatagat catgtcctgc
    70021 cactgcttca ccatccccag atagcggtta ttgagattca ccaccagtac gggcaactcg
    70081 tattgcaacg cggtagacag ttcctggatg ttcatctgaa tactgccgtc gccagtgacg
    70141 caaaccacgg tttcttctgg caacgccatt ttgacgccca gtgccgcagg taaaccaaaa
    70201 cccatcgtgc cgaggccacc ggaattgatc cagcgacgcg gtttgtcgaa tggataataa
    70261 agtgcagcaa acatctggtg ctgcccgaca tcggacgtca cgtaagcgtc tcccttcgtc
    70321 aaccgccaaa gagtctcgat caccgcctgc ggtttaatct tttcactgtg agtgtcatat
    70381 ttcaggcact gacgagcgcg ccactgttca atttgctgcc accagtcgcg gatctcatcc
    70441 agtggttgat gggcggattc ttgcgacaag agttcaagca tttgttcgag gacctggcga
    70501 gcatccccca caatcgggat atccgcagtc acggttttag aaatggaagt aggatcaata
    70561 tcgatatgca gaacagtggc atttgggcag tactttgcca gattgttcgt cgttcggtca
    70621 tcaaatcgta ccccgacggc gaaaatcaca tccgcgttat gcatcgtcat attggcttcg
    70681 taggtaccgt gcattcccag catgcccagt gcctgacgat gcgttgccgg aaacgccccc
    70741 agccccatca atgagcaaac aacgggcaga ttcaacgcct ccaccgtttc tttcaactgc
    70801 tgatggcagc ccgccgtgat tgccccaccg cctacgtaga caaccggttt ttttgccgct
    70861 accagcgttt gcagagcacg cttaatttgc cctttatgtc cggtagtagt gggattgtaa
    70921 gaacgcatac tgaccgactc cggccagaca tagggtaatt tgttcgccgg attaagaata
    70981 tctttcggta aatcaacgac tactggtcct gggcgaccac ttgccgccag ccagaaagcc
    71041 tttttcagca cctgcggaat gtcttccgtt tgcttaacca gaaaactgtg tttaaccacc
    71101 ggtcgcgaaa tccccaccat gtcgcactcc tgaaaggcat cgtaacctat caacgaggtc
    71161 gctacctgcc cggaaaggac aactaatgga atggaatcca tataagcggt ggcgatgcca
    71221 gtaatcgcat tggtcgcccc tggacccgac gttaccagca cgacgccgac ttccccggtc
    71281 gcgcgcgcca ggccatcggc catatgcacc gccgcctgct catgacgaac taatacatga
    71341 tcaataccac ccacggtatg caatgcatca taaatatcaa ggactgcgcc tccgggataa
    71401 ccgaatactt gtttaacgcc ctgatcgata agcgatcgga cgaccatctc ggctccagac
    71461 aacatctcca tggcctgcct cactgtttga cggaaaaaat gtgtaaaaca cattaaccgt
    71521 ccgatcttgc ttaagcaatt ggcagccaga gacataaaca cggggtaata agaataaaca
    71581 ggaggaaagg tgaaaagaaa cgataaaatc ctccattaca gaggattaaa ttgataataa
    71641 atcggctgaa tcccacaact tacagcaatt tctctatttt tttaattaat cctaaaatag
    71701 agagcccaca cattcagaat aaaattctac attcaataaa aaatgcagaa taaaccagac
    71761 attcatgtct gacctattct gcaatcagtt agcgtttgca aattgagact aattgctctt
    71821 ccatccactg atggccttta tcgcgcccgg cagcttcatg ccaggagaga taacaggttc
    71881 tgctgttttg ttttaacggc agcggtaata cctgtaattc taaggattca gcgaactctt
    71941 cagccagcca acgcggcgca atagcgacca aatgcgtttg cgacaccacg ctaagtacgc
    72001 tcatcattgc catgccctga tacgcgatac tggcttgctt atctaccgtg tcataccaag
    72061 gttgactaaa tgacgcgaaa cgatcgagcg aaaccgccgc atgttgttcg ttataaacat
    72121 catgtttcag taacgggccc ttaattgttg gatgattttt gctggctacc agcaccattt
    72181 catctttaaa taatggtacg ctggtaaatt caggacgatg gaagtcttca taactaatca
    72241 caaactccgt ttcctgataa cgcagctgat gttcagtgtt ctgatttaat gaagacttga
    72301 acataacatg tatatttggc gcaatctgct caatgtgatt ataaatctgc gaggtcagaa
    72361 tgctgtctaa cgggctgcaa acacaaagat gaaatacacg ttcactgctc gcgggttcaa
    72421 aacctgaacc aggcaattca ttttgtacta gttgcaatgc ctgacgaact gaaccaaaaa
    72481 gttgaaatgc gcgagcagtc ggttgaatac cacggccata acgaacaaaa agctcgtcat
    72541 taaacatcac cttcaggcgt gcaacagcgt tactgaccgc aggttgcgac attcccagaa
    72601 catgagcggc acgagtaatg ttttgctcct gcatcacggc atcgaaaacg gttaataagt
    72661 tgagatcgac catgcgtagc tgtggtttgc ttaactccgc cgtctctgga tgatctgttt
    72721 gtacctctgg catacttaac tccactgtca cacttaactc cctttccctt attggaatgc
    72781 agagaaataa tcctgaagaa tatgatttat catgcatata aaataagaaa aagcaaaatg
    72841 agtaaaattc ggaaaacata aagatacggg ttctgataaa acagaatcca ttgccgtgga
    72901 atgagtcatt taccacagca taataatcca taatgttttt gttgcgaaaa caatctaa
//
LOCUS       DBBGLQ010000019        67228 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.019, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000019 DBBGLQ010000000
VERSION     DBBGLQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.019"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            36..1013
                     /gene="ybeQ"
                     /locus_tag="ACIYSB_001580"
     CDS             36..1013
                     /gene="ybeQ"
                     /locus_tag="ACIYSB_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HGC5428658.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYRHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1083..1565)
                     /gene="ybeL"
                     /locus_tag="ACIYSB_001581"
     CDS             complement(1083..1565)
                     /gene="ybeL"
                     /locus_tag="ACIYSB_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGC5428659.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1800..4382
                     /gene="leuS"
                     /locus_tag="ACIYSB_001582"
     CDS             1800..4382
                     /gene="leuS"
                     /locus_tag="ACIYSB_001582"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGC5428660.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTENQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4397..4978
                     /gene="lptE"
                     /locus_tag="ACIYSB_001583"
     CDS             4397..4978
                     /gene="lptE"
                     /locus_tag="ACIYSB_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGC5428661.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4978..6009
                     /gene="holA"
                     /locus_tag="ACIYSB_001584"
     CDS             4978..6009
                     /gene="holA"
                     /locus_tag="ACIYSB_001584"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGC5428662.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6011..6652
                     /gene="nadD"
                     /locus_tag="ACIYSB_001585"
     CDS             6011..6652
                     /gene="nadD"
                     /locus_tag="ACIYSB_001585"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGC5428663.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWQEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6676..7287
                     /gene="cobC"
                     /locus_tag="ACIYSB_001586"
     CDS             6676..7287
                     /gene="cobC"
                     /locus_tag="ACIYSB_001586"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGC5428664.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7547..7864
                     /gene="rsfS"
                     /locus_tag="ACIYSB_001587"
     CDS             7547..7864
                     /gene="rsfS"
                     /locus_tag="ACIYSB_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGC5428665.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7868..8335
                     /gene="rlmH"
                     /locus_tag="ACIYSB_001588"
     CDS             7868..8335
                     /gene="rlmH"
                     /locus_tag="ACIYSB_001588"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGC5428666.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8366..10267
                     /gene="mrdA"
                     /locus_tag="ACIYSB_001589"
     CDS             8366..10267
                     /gene="mrdA"
                     /locus_tag="ACIYSB_001589"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGC5428667.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10270..11382
                     /gene="mrdB"
                     /locus_tag="ACIYSB_001590"
                     /gene_synonym="rodA"
     CDS             10270..11382
                     /gene="mrdB"
                     /locus_tag="ACIYSB_001590"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGC5428668.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11393..12481
                     /gene="rlpA"
                     /locus_tag="ACIYSB_001591"
     CDS             11393..12481
                     /gene="rlpA"
                     /locus_tag="ACIYSB_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGC5428669.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAVSQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12620..13831
                     /gene="dacA"
                     /locus_tag="ACIYSB_001592"
     CDS             12620..13831
                     /gene="dacA"
                     /locus_tag="ACIYSB_001592"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGC5428670.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13941..14204
                     /gene="ybeD"
                     /locus_tag="ACIYSB_001593"
     CDS             13941..14204
                     /gene="ybeD"
                     /locus_tag="ACIYSB_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGC5428671.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14305..14946
                     /gene="lipB"
                     /locus_tag="ACIYSB_001594"
     CDS             14305..14946
                     /gene="lipB"
                     /locus_tag="ACIYSB_001594"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGC5428672.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15205..16158
                     /gene="ybeF"
                     /locus_tag="ACIYSB_001595"
     CDS             15205..16158
                     /gene="ybeF"
                     /locus_tag="ACIYSB_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGC5428673.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16367..17332
                     /gene="lipA"
                     /locus_tag="ACIYSB_001596"
     CDS             16367..17332
                     /gene="lipA"
                     /locus_tag="ACIYSB_001596"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGC5428674.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17433..17636)
                     /gene="tatE"
                     /locus_tag="ACIYSB_001597"
     CDS             complement(17433..17636)
                     /gene="tatE"
                     /locus_tag="ACIYSB_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGC5428675.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17765..18552)
                     /locus_tag="ACIYSB_001598"
                     /pseudo
     CDS             complement(17765..18552)
                     /locus_tag="ACIYSB_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
     gene            18645..19028
                     /gene="crcB"
                     /locus_tag="ACIYSB_001599"
     CDS             18645..19028
                     /gene="crcB"
                     /locus_tag="ACIYSB_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGC5428676.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGIGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19082..19291)
                     /gene="cspE"
                     /locus_tag="ACIYSB_001600"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19082..19291)
                     /gene="cspE"
                     /locus_tag="ACIYSB_001600"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGC5428677.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19466..20026)
                     /gene="pagP"
                     /locus_tag="ACIYSB_001601"
     CDS             complement(19466..20026)
                     /gene="pagP"
                     /locus_tag="ACIYSB_001601"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGC5428678.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            20615..22000
                     /gene="dcuC"
                     /locus_tag="ACIYSB_001602"
     CDS             20615..22000
                     /gene="dcuC"
                     /locus_tag="ACIYSB_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGC5428679.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(22041..22721)
                     /gene="dpiA"
                     /locus_tag="ACIYSB_001603"
                     /gene_synonym="citB"
     CDS             complement(22041..22721)
                     /gene="dpiA"
                     /locus_tag="ACIYSB_001603"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGC5428680.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(22690..24348)
                     /gene="dpiB"
                     /locus_tag="ACIYSB_001604"
     CDS             complement(22690..24348)
                     /gene="dpiB"
                     /locus_tag="ACIYSB_001604"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGC5428681.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIVEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            24727..25785
                     /gene="citC"
                     /locus_tag="ACIYSB_001605"
     CDS             24727..25785
                     /gene="citC"
                     /locus_tag="ACIYSB_001605"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGC5428682.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            25800..26096
                     /gene="citD"
                     /locus_tag="ACIYSB_001606"
     CDS             25800..26096
                     /gene="citD"
                     /locus_tag="ACIYSB_001606"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGC5428683.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            26093..27001
                     /gene="citE"
                     /locus_tag="ACIYSB_001607"
     CDS             26093..27001
                     /gene="citE"
                     /locus_tag="ACIYSB_001607"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGC5428684.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDAPVIDRARLVLSRAELSGIREE"
     gene            27012..28544
                     /gene="citF"
                     /locus_tag="ACIYSB_001608"
     CDS             27012..28544
                     /gene="citF"
                     /locus_tag="ACIYSB_001608"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGC5428685.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            28548..29099
                     /gene="citX"
                     /locus_tag="ACIYSB_001609"
     CDS             28548..29099
                     /gene="citX"
                     /locus_tag="ACIYSB_001609"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGC5428686.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            29074..29952
                     /gene="citG"
                     /locus_tag="ACIYSB_001610"
     CDS             29074..29952
                     /gene="citG"
                     /locus_tag="ACIYSB_001610"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGC5428687.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGAYSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            30003..31466
                     /gene="citT"
                     /locus_tag="ACIYSB_001611"
     CDS             30003..31466
                     /gene="citT"
                     /locus_tag="ACIYSB_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGC5428688.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            31580..32386
                     /gene="rna"
                     /locus_tag="ACIYSB_001612"
     CDS             31580..32386
                     /gene="rna"
                     /locus_tag="ACIYSB_001612"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGC5428689.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            32616..33026
                     /gene="rnk"
                     /locus_tag="ACIYSB_001613"
     CDS             32616..33026
                     /gene="rnk"
                     /locus_tag="ACIYSB_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGC5428690.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            33164..33253
                     /gene="yldA"
                     /locus_tag="ACIYSB_001614"
     CDS             33164..33253
                     /gene="yldA"
                     /locus_tag="ACIYSB_001614"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGC5428691.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(33257..34495)
                     /gene="ybdR"
                     /locus_tag="ACIYSB_001615"
     CDS             complement(33257..34495)
                     /gene="ybdR"
                     /locus_tag="ACIYSB_001615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGC5428692.1"
                     /translation="MKALTYHGPHHVQVENVPDPGVEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLHFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLSFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            34716..35144
                     /gene="uspG"
                     /locus_tag="ACIYSB_001616"
     CDS             34716..35144
                     /gene="uspG"
                     /locus_tag="ACIYSB_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGC5428693.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAQERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(35265..36830)
                     /gene="ahpF"
                     /locus_tag="ACIYSB_001617"
     CDS             complement(35265..36830)
                     /gene="ahpF"
                     /locus_tag="ACIYSB_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGC5428694.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(37075..37638)
                     /gene="ahpC"
                     /locus_tag="ACIYSB_001618"
     CDS             complement(37075..37638)
                     /gene="ahpC"
                     /locus_tag="ACIYSB_001618"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGC5428695.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            38010..38756
                     /gene="dsbG"
                     /locus_tag="ACIYSB_001619"
     CDS             38010..38756
                     /gene="dsbG"
                     /locus_tag="ACIYSB_001619"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGC5428696.1"
                     /translation="MLKKILLLALLPAIVFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            38965..39867
                     /gene="ybdO"
                     /locus_tag="ACIYSB_001620"
     CDS             38965..39867
                     /gene="ybdO"
                     /locus_tag="ACIYSB_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5428697.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            40014..41234
                     /gene="ybdN"
                     /locus_tag="ACIYSB_001621"
                     /pseudo
     CDS             40014..41234
                     /gene="ybdN"
                     /locus_tag="ACIYSB_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415135.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            41207..41836
                     /gene="ybdM"
                     /locus_tag="ACIYSB_001622"
     CDS             41207..41836
                     /gene="ybdM"
                     /locus_tag="ACIYSB_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGC5428698.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(41837..42997)
                     /gene="ybdL"
                     /locus_tag="ACIYSB_001623"
     CDS             complement(41837..42997)
                     /gene="ybdL"
                     /locus_tag="ACIYSB_001623"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGC5428699.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAHHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            43106..44194
                     /gene="hcxA"
                     /locus_tag="ACIYSB_001624"
     CDS             43106..44194
                     /gene="hcxA"
                     /locus_tag="ACIYSB_001624"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGC5428700.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFKKVESFKA"
     gene            complement(44204..44401)
                     /gene="ybdD"
                     /locus_tag="ACIYSB_001625"
     CDS             complement(44204..44401)
                     /gene="ybdD"
                     /locus_tag="ACIYSB_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGC5428701.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(44584..46689)
                     /gene="cstA"
                     /locus_tag="ACIYSB_001626"
     CDS             complement(44584..46689)
                     /gene="cstA"
                     /locus_tag="ACIYSB_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGC5428702.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(46870..47283)
                     /gene="entH"
                     /locus_tag="ACIYSB_001627"
     CDS             complement(46870..47283)
                     /gene="entH"
                     /locus_tag="ACIYSB_001627"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGC5428703.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDGVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(47286..48032)
                     /gene="entA"
                     /locus_tag="ACIYSB_001628"
     CDS             complement(47286..48032)
                     /gene="entA"
                     /locus_tag="ACIYSB_001628"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGC5428704.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(48032..48889)
                     /gene="entB"
                     /locus_tag="ACIYSB_001629"
     CDS             complement(48032..48889)
                     /gene="entB"
                     /locus_tag="ACIYSB_001629"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGC5428705.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(48903..50513)
                     /gene="entE"
                     /locus_tag="ACIYSB_001630"
     CDS             complement(48903..50513)
                     /gene="entE"
                     /locus_tag="ACIYSB_001630"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGC5428706.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALIEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(50523..51698)
                     /gene="entC"
                     /locus_tag="ACIYSB_001631"
     CDS             complement(50523..51698)
                     /gene="entC"
                     /locus_tag="ACIYSB_001631"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGC5428707.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            52073..53029
                     /gene="fepB"
                     /locus_tag="ACIYSB_001632"
     CDS             52073..53029
                     /gene="fepB"
                     /locus_tag="ACIYSB_001632"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGC5428708.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(53033..54283)
                     /gene="entS"
                     /locus_tag="ACIYSB_001633"
     CDS             complement(53033..54283)
                     /gene="entS"
                     /locus_tag="ACIYSB_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGC5428709.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRHFRQTPPQVTASDS"
     gene            54394..55398
                     /gene="fepD"
                     /locus_tag="ACIYSB_001634"
     CDS             54394..55398
                     /gene="fepD"
                     /locus_tag="ACIYSB_001634"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGC5428710.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAIVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            55395..56387
                     /gene="fepG"
                     /locus_tag="ACIYSB_001635"
     CDS             55395..56387
                     /gene="fepG"
                     /locus_tag="ACIYSB_001635"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGC5428711.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            56384..57199
                     /gene="fepC"
                     /locus_tag="ACIYSB_001636"
     CDS             56384..57199
                     /gene="fepC"
                     /locus_tag="ACIYSB_001636"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGC5428712.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(57196..58329)
                     /gene="wzz(fepE)"
                     /locus_tag="ACIYSB_001637"
     CDS             complement(57196..58329)
                     /gene="wzz(fepE)"
                     /locus_tag="ACIYSB_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGC5428713.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKSFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(58545..62426)
                     /gene="entF"
                     /locus_tag="ACIYSB_001638"
     CDS             complement(58545..62426)
                     /gene="entF"
                     /locus_tag="ACIYSB_001638"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGC5428714.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQLNATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGTFEKIGPIIRATLNR"
     gene            complement(62423..62641)
                     /gene="ybdZ"
                     /locus_tag="ACIYSB_001639"
     CDS             complement(62423..62641)
                     /gene="ybdZ"
                     /locus_tag="ACIYSB_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HGC5428715.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCVLPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            complement(62644..63846)
                     /gene="fes"
                     /locus_tag="ACIYSB_001640"
     CDS             complement(62644..63846)
                     /gene="fes"
                     /locus_tag="ACIYSB_001640"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGC5428716.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            64089..66329
                     /gene="fepA"
                     /locus_tag="ACIYSB_001641"
     CDS             64089..66329
                     /gene="fepA"
                     /locus_tag="ACIYSB_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGC5428717.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSTTDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            66504..67124
                     /gene="entD"
                     /locus_tag="ACIYSB_001642"
     CDS             66504..67124
                     /gene="entD"
                     /locus_tag="ACIYSB_001642"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGC5428718.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
BASE COUNT        16401 a        17820 c        16977 g        16030 t
ORIGIN      
        1 taatatgttt tatctgcaac cataaggaaa tgataatgat tttcacgtca agttgctgcg
       61 ataatttatc aatagatgag attatcgaac gtgctgaaaa aggcgattgc gaggctcaat
      121 atattgttgg gttttattat aatcgcgata gcgcaattga ttctccggac gacgaaaaag
      181 ccttttactg gctgaagctg gccgctgagc aaggtcattg tgaagcacag tattccttag
      241 ggcaaaagta taccgaggat aaaagccgtc ataaagataa tgagcaagcc atcttctggc
      301 tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa cgcccttggc tggacactgg
      361 atcgtggaga agcccccaat tataaagaag cggttgtctg gtatcagata gccgcggaga
      421 gcggaatgtc ttatgcgcaa aataatcttg ggtggatgta cagaaatggc aacggagtcg
      481 caaaagacta tgcgctggca tttttctggt acaaacaagc tgcattacaa ggccatagtg
      541 acgcgcaaaa caatctggcc gatctttatg aagacggaaa aggcgttgct caaaacaaga
      601 cactcgccgc attctggtat ttgaaaagcg cacagcaggg taatcggcac gcccagtttc
      661 aaattgcatg ggattataac gctggcgaag gggtggacca ggactataag caagcgatgt
      721 actggtatct gaaggctgcc gctcagggga gcgtcggcgc ttacgtcaac atcggttata
      781 tgtatagaca cggacaaggc gttgagaaag attatcaggc tgcctttgaa tggtttacga
      841 aagccgctga atgcaatgac gccactgcct ggtataacct ggccattatg tatcactacg
      901 gagaaggaag acctgtcgat ctccgacagg ctctcgacct gtatcgtaaa gttcagtcat
      961 ccggaaccag agatgtcagt caggaaattc gtgagactga agatttactg tagagataaa
     1021 aacaagggcc ggggaatttc attcggccca tattctcttt attgctgtgc gaaatcagac
     1081 tattacggct caaacgggcg tctctggaac tggtcatgac cacatttcgg gcatagcgtc
     1141 agcacttccg gtgtgtagat cgggagatgg aagtgacatt tctcgcagac cagatttccc
     1201 agcccgacca cttctccgct gtgataaacc ccatgatgat tgaggtcctg gaaaacttcg
     1261 cgccattcaa gctgcgtttt atcggtgatg tctgccagct cctgccacaa gctttcttta
     1321 atcacccgca taaagacgct gtcagattct tctttcaggc tctcttcata gctcatggcg
     1381 aactcttcca ggtcacgtct gacagctcgc gtcagctcat cgacctcggt tcgcgttaac
     1441 tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca gtgcgtcgat atcacgttcg
     1501 ccattgcgca ggcgttcgct cagtgacgca accagttcac ggtaatattg agcaaccttg
     1561 ttcatcgttt catctcccgg gtcgttaact gactataata atagacgttc tttgttcctt
     1621 gctttgctaa tacgcccaca aactctgtaa attaatgtat gaataatccg ggcatcattt
     1681 caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt tgggctatgc tatgcggatc
     1741 tgaaaaacca cctcaacgct acatttgtag ccgtattgaa aacaggacca ctggctgcca
     1801 tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat tgggatgaga
     1861 agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc ctgtctatgc
     1921 ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc atcggtgacg
     1981 tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct gcagccgatc ggctgggacg
     2041 cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg gcaccgtgga
     2101 cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat gctgggcttt ggttatgact
     2161 ggagccgcga gctggcaacc tgtacgccgg aatactaccg ttgggaacag aaattcttca
     2221 ccgagctgta taaaaaaggc ctggtatata agaagacttc tgcggtcaac tggtgcccga
     2281 acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata
     2341 ccaaagttga acgtaaagag atcccgcagt ggtttatcaa aatcactgct tacgctgacg
     2401 agctgctcaa cgatctggat aaactggatc actggccaga caccgttaaa accatgcagc
     2461 gtaactggat cggtcgttcc gaaggcgtgg agatcacctt caacgttaac gactatgaca
     2521 acacgctgac cgtttacact acccgcccgg acacctttat gggttgtacc tacctggcgg
     2581 tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa ctggcggcct
     2641 ttattgacga atgccgtaac accaaagttg ccgaagctga aatggcgacg atggagaaaa
     2701 aaggcgtcga tactggcttt aaagcggttc acccattaac gggcgaagaa attcccgttt
     2761 gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agttatggcg gtaccggggc
     2821 acgaccagcg cgactacgag tttgcctcta aatacggcct gaacatcaaa ccggttatcc
     2881 tggcagctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa aaaggcgtgc
     2941 tgttcaactc tggcgagttc aacggtcttg accatgaagc ggccttcaac gccatcgccg
     3001 ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg cgcgactggg
     3061 gtgtttcccg tcagcgttac tggggcgcgc cgattccgat ggtgacgctg gaagacggta
     3121 ccgtaatgcc gaccccggac gaccagctgc cggtgatcct gccggaagat gtggtaatgg
     3181 acggcattac cagcccgatt aaagcagatc cggagtgggc gaaaactacc gttaacggta
     3241 tgccagcact gcgtgaaacc gacactttcg acacctttat ggagtcctcc tggtactatg
     3301 cgcgctacac ttgcccgcag tacaaagaag gtatgctgga ttccgaagcg gctaactact
     3361 ggctgccggt ggatatctac attggtggta ttgaacacgc cattatgcac ctgctctact
     3421 tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac gaaccagcga
     3481 aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt ggcgaaaacg
     3541 gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag aaaggccgta
     3601 tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg agcaaaatgt
     3661 ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac ggcgcggaca
     3721 ccgttcgtct gtttatgatg tttgcttctc cggctgatat gactctcgaa tggcaggaat
     3781 ccggtgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtt tacgagcaca
     3841 cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct gactgaaaat cagaaagcgc
     3901 tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga tgatatcggc cgtcgtcaga
     3961 ccttcaacac cgcaattgcg gcgattatgg agctgatgaa caaactggcg aaagcaccaa
     4021 ccgatggcga gcaggatcgc gctctgatgc aggaagcact gctggccgtt gtccgtatgc
     4081 ttaacccgtt caccccgcac atctgcttca cgctgtggca ggaactgaaa ggcgaaggcg
     4141 atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc gatggtggaa gactccacgc
     4201 tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat caccgttccg gtggacgcaa
     4261 cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa tatcttgatg
     4321 gcgttactgt acgtaaagtg atttacgtac caggtaaact cctcaatctg gtcgttggct
     4381 aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg cggtgttaat
     4441 caccgccggg tgtggctggc atctgcgtga taccacgcag gttccttcca ctatgaaggt
     4501 catgatcctg gactcaggcg atccgaacgg gccattaagc cgtgcggtgc gtaaccagtt
     4561 acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg ttccatcctt
     4621 gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg gtattccgta acggtcaaac
     4681 agcagagtat cagatgatca tgacggttaa tgcgaccgtg ttgatccccg gccgtgatat
     4741 ctacccgatt agcgccaaag tcttccgttc gttcttcgat aacccgcaaa tggcgttagc
     4801 gaaagataac gaacaagaca tgatcgtaaa agagatgtac gaccgtgctg ccgaacagct
     4861 gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag aacagacgtc
     4921 gaccacaacg gatactccgg caacgcctgc acgcgtctcc accacgctgg gtaactgatg
     4981 attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg cgcggcgtat
     5041 cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctgt tcgtcaggta
     5101 gctgcggcac aaggattcga agaacaccac actttttcca ttgatcccaa cactgactgg
     5161 aatgcgatct tttcgttatg ccaggctatg agtctgtttg ccagtcgaca aacgctattg
     5221 ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg agcaacttct cacactcacc
     5281 ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag caaagcgcaa
     5341 gaaaatgccg cctggtttac tgcgcttgcg aatcgcagcg tgcaggtgac ctgtcagaca
     5401 ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg caaaacagct caacttagaa
     5461 ctggatgacg cggcaaatca ggtgctctgc tactgttatg aaggtaacct gctggcgctg
     5521 gctcaggcac tggagcgttt atcgctgctc tggccagacg gcaaattgac attaccgcgc
     5581 gttgaacagg cggtgaatga tgccgcgcat ttcacccctt ttcattgggt tgatgctttg
     5641 ttgatgggaa aaagtaagcg cgcattgcat attcttcagc aactgcgtct ggaaggcagc
     5701 gaaccggtta ttttgttgcg cacattacaa cgtgaactgt tgttactggt taacctgaaa
     5761 cgccagtctg cccatacgcc actgcgtgcg ttgtttgata agcatcgggt atggcagaac
     5821 cgccggggca tgatgggcga ggcgttaaat cgcttaagtc agacgcagtt acgtcaggcc
     5881 gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacggtca gtcagtgtgg
     5941 gcagagctgg aagggttatc tcttctgttg tgccataaac ccctggcgga cgtatttatc
     6001 gacggttgat atgaaatctt tacaggctct gtttggcggc acctttgatc cggtgcacta
     6061 tggtcatcta aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc gggtcacaat
     6121 catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg tgcagcgtaa
     6181 acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg aacgcgagct
     6241 aaagcgcaat gccccctctt acactgcgca aacactgaaa gagtggcggc aggaacaagg
     6301 accggacgtg ccgctggcgt ttattattgg tcaggattca ctgctgacct ttccgacctg
     6361 gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc gtccaggtta
     6421 cccacttgaa atggcgcaac cgcaatacca gcaatggcag gaagatcatt tgacacataa
     6481 cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa cgccgtggtt
     6541 taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcat gtgaggattt
     6601 attgccggaa ccggtactga cttacattaa ccaacaaggc ttgtatcgct gatacctgtc
     6661 gttacggagg gcatgatgcg actgtggtta attcgtcatg gtgaaacgca agcgaatatc
     6721 gatggtcttt acagcggtca tgcgcccacc cccctgaccg cgcgcggtat tgagcaagcg
     6781 caaaatctgc atacgctgct acatggtgtt tcctttgatc tggttttatg cagtgaactg
     6841 gaacgggcac agcataccgc gcgactggtt ctcagtgacc gccagctccc cgtgcaaatc
     6901 atacctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca tcgcgacctc
     6961 atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca tgcaatcccc
     7021 acgaacggtg aaggatttca ggcattttcg caacgtgtgg aacgctttat cgcaaggctt
     7081 agtgaatttc agcactatca gaatatttta gtcgtcagcc atcagggtgt actgagtctg
     7141 ttaatcgccc gtttaattgg catgcctgcc gaggctatgt ggcattttcg cgttgaccag
     7201 gggtgttgga gcgccattga tatcaaccaa aaattcgcga cgctacgcgt cctcaatagc
     7261 cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa gccgcaacga
     7321 ccattgacag acccgggcag gctgatattc tccgcagcca gactttttcc gccagacacg
     7381 actttgtaga aattgtttta caaaaatggc gatgctatct gcggcgcggg gtgggatgat
     7441 agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct ggttcaggta
     7501 tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc agggtaaagc
     7561 actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca tcatcgcctt
     7621 agacgttcag ggcaaatcca gcatcaccga ctgcatgatc atctgtacgg gtacgtccag
     7681 ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag cgggcctgtt
     7741 accgctcggc gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt tgggcgatgt
     7801 gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa aactctggag
     7861 ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact gggtacaaac
     7921 cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc tgattgaaat
     7981 tccggccgga aaacgcggca agaatgcgga catcaagcgc atactcgaca aagagggtga
     8041 gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc caggcaagcc
     8101 ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aagctggatg gtcgcgacgt
     8161 cagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg cggctgagca
     8221 gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg gttcgcgtgc tggtcgcaga
     8281 gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg agtgataagg
     8341 gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg actatacggc
     8401 agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt tgctgctgac
     8461 cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg actaccagac
     8521 ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg gcattatcta
     8581 cgatcgtaac ggtatccctc tggccctcaa ccgcactatc taccagatag aaatgatgcc
     8641 ggagaaagtc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg tagatctgac
     8701 cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt tcacctctat
     8761 tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca atcagtaccg
     8821 ttttccgggt gtcgaagtta aaggctataa acgtcgttac tatccttacg gttcggcgtt
     8881 gacccacgtc atcggctatg tgtcgaaaat caacgataaa gacgtcgaac gcctgaataa
     8941 tgacggcaaa ctggccaact atgcggcaac gcatgatatc ggtaagctgg gcattgagcg
     9001 ttactatgaa gatgtgctgc acggtcagac cggttatgaa gaggttgaag ttaacaaccg
     9061 tgggcgtgtt attcgccagt taaaagaagt accaccgcaa gccggacacg atatttacct
     9121 gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta gccgcgcagc
     9181 tgtggtagtc accgatccgc gtacaggtgg ggtgctggcg ctggtttcca cgcctagtta
     9241 tgacccaaac ttgtttgttg acggtatctc cagcaaagat tattccgcct tgttgaacga
     9301 tccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg cgtctacagt
     9361 taaaccctat gtggcggttt cggcattgag cgccggggtg atcacgcgca atacgacgct
     9421 gtttgaccca ggctggtggc aactgccagg ttcggaaaaa cgttatcgtg actggaaaaa
     9481 atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg ataccttctt
     9541 ctatcaggtg gcctacgata tggggatcga tcgcctctcc gaatggatgg gtaaattcgg
     9601 ttatggtcat tacaccggta tcgacctggc ggaagaacgt tccggcaaca tgcctacccg
     9661 cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca ttccggttgg
     9721 tatcggtcag ggttactgga cagcgacccc aatccagatg agtaaggcac tgatgatcct
     9781 gattaatgac ggtatcgtga aggttcctca tttgctgatg agcaccgccg aagacggcaa
     9841 acaggtgcca tgggtacagc cgcatgaacc gcccgtcggc gatattcatt ccggttactg
     9901 ggagctggcg aaagacggta tgtacggtgt tgctaaccgc cctaacggta cggcgcataa
     9961 atactttgct agcgcaccgt acaaaattgc ggcgaaatcc ggtaccgctc aggtcttcgg
    10021 tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac gtgaccacaa
    10081 actgatgacc gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca tgattctgga
    10141 gaacggtggt gcgggtccgg cggttggtac actgatgcgc cagatcctcg accacattat
    10201 gctgggtgat aacaacaccg atctgcctgc ggaaaatcca gcggttgccg cagcggagga
    10261 ccattaatca tgacggataa tccgaataaa aaaacattct gggataaagt ccatctcgat
    10321 cccacaatgc tgctgatctt actggcattg ctggtttaca gcgccctggt tatctggagc
    10381 gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc gatgggtctg
    10441 gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg ggccccctat
    10501 ctctatatca tctgtattat tttgctggtg gcggtagatg ctttcggtgc catctctaaa
    10561 ggtgctcaac gctggctgga cctcggtatt gttcgttttc agccgtcgga aattgccaaa
    10621 atagccgtac cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc gccatcgttg
    10681 aagaacactg gcatcgcgct ggtgctgata tttatgccca cgctgctggt ggctgcacag
    10741 cctgacctgg gaacatcaat cctcgttgcg ctttccggtc tgtttgtact gttcctctct
    10801 ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tagcggcgtt cattccgatt
    10861 ctgtggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct cctggacccg
    10921 gaatcagacc cactcggcgc gggctatcac attattcagt ctaaaattgc tattggctcc
    10981 ggcggattac gcggcaaagg ctggctgcac ggcactcagt cacagcttga atttctcccc
    11041 gaacgccata ctgactttat cttcgcggta ctggcggaag agctgggatt agtgggcatt
    11101 ctgattctgc tcgctctcta cattctgctg atcatgcgcg ggctgtggat agccgccaga
    11161 gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa tgctgatatt attcgtttat
    11221 gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt tccgctccca
    11281 ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg gattgtaatg
    11341 tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg caatgcgtaa
    11401 gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa gcgatgatgg
    11461 tcagcaacag acggtaagtg taccgcagcc tgcggtatgt aacggcccta tagttgaaat
    11521 tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg attaccagcg
    11581 cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttagccagg cgggactggc
    11641 ggcaatctat gatgccgaac ccggcagtaa cctgacggcc tctggcgaag cattcgatcc
    11701 gacacagctg acggcagccc atccaacgct tccgatcccc agctacgcca gaatcactaa
    11761 cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggccctt acggcaacga
    11821 ccgcgttatt tcactttctc gcgcggcagc tgaccgtctt aacacgtcaa acaacaccaa
    11881 agttcgtatc gatccgatta ttgttgccca ggatggttcg ctttctggtc ctggtatggc
    11941 ttgtaccaca gtcgccaaac agacttacgc cctgcctgca cctcccgatt taagcggtgg
    12001 cgcgggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca gtaattcgac
    12061 gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt agcggtttcc tcggcgcacc
    12121 aacgacctta gcgcctggtg tactggaagg cagcgaaccg acgcctgcgc cacagcccgt
    12181 tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca atggtgacac cgcaagccgt
    12241 ctcgcaaagc gccagcggca actttatggt gcaagtcggg gccgtaagcg atcaggctcg
    12301 tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc gcgtaactca
    12361 aaatggcgcg gtctggcgga tccagcttgg cccattcgcc agcaaagccg aagccagtac
    12421 cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacta ccgcgcagta
    12481 gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa gtcacgcgga
    12541 aagtcagatg cctgccggta gtgcatttgc tatagtaggg cactttttta attccatcac
    12601 ggatgtcgta gttcagacca tgaataccat tttttccgct cgtatcatga agcgcctggc
    12661 gctcaccacg gctctttgca cagcctttat ctctgctgca catgccgatg acctgaatat
    12721 caaaactatg atcccgggtg taccgcagat cgatgcggag tcctacatcc tgattgacta
    12781 taactccggc aaagtgctcg ccgaacagaa cgcagatgtc cgccgcgatc ctgccagcct
    12841 gaccaaaatg atgaccagtt acgttatcgg ccaggcaatg aaagccggta aatttaaaga
    12901 aactgattta gtcactatcg gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg
    12961 ttcttcgctg atgttcctca aaccgggcat gcaggttccg gtttctcagc tgatccgcgg
    13021 tattaacctg caatcgggta acgatgcttg tgtcgccatg gccgattttg ccgctggtag
    13081 ccaggacgct tttgttggct tgatgaacag ctacgttaac gcactgggcc tgaaaaatac
    13141 ccacttccag acggtacatg gtctggatgc tgatggtcag tacagctccg cgcgagatat
    13201 ggcgctgatc ggccaggcat tgatccgtga cgtaccgaat gaatactcga tctataaaga
    13261 aaaagaattt acgtttaacg gtattcgcca gctgaaccgt aacggcctgt tatgggataa
    13321 cagcctgaat gtcgacggca tcaaaaccgg acacactgac aaagcaggtt acaaccttgt
    13381 tgcttctgcg actgaaggcc agatgcgctt gatttctgcg gtaatgggcg gacgtacttt
    13441 taaaggccgt gaagccgaaa gtaaaaaact gctaacctgg ggcttccgtt tctttgaaac
    13501 cgttaaccca ctgaaagtag gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc
    13561 tgatcgcgct tcgttagggg ttgataaaga cgtgtacctg accattccgc gtggtcgcat
    13621 gaaagatctg aaagccagct atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa
    13681 gaatcaggtc gtcggaacta tcaacttcca gcttgatggc aaaacgatcg agcaacgccc
    13741 gctggttgtg ttgcaagaaa tcccggaagg taacttcttc ggcaaaatca ttgattacat
    13801 taaattaatg ttccatcact ggtttggtta aaaattaaac acttgaaagt gtaatttccg
    13861 tccccatata ctaagcatca gtaaaaaact cccgccttct ggcgggagtt gctatttaat
    13921 tacgttacgc cggagctgac atgaaaacca aacttaacga actgcttgaa ttccctactc
    13981 cttttactta caaagttatg gggcaggcgt tacctgagct ggttgatcag gtggttgaag
    14041 tggtacagcg ccatgcgcca ggtgactaca ccccaacggt aaaaccaagc agcaaaggca
    14101 actaccactc ggtatctatc actatcaacg ccactcatat cgagcaggtt gaaacactgt
    14161 atgaagaact gggcaaaatc gatattgtcc gcatggttct gtaactcgct tctccgttac
    14221 ccggctcctg gtcgggtaac tcccctctcc tcgctgtgat atacttttcc cccactttta
    14281 ctcattctcc acggagatgc cgttttgtat caggataaaa ttcttgtccg ccagctcggt
    14341 cttcagcctt acgagccaat ctcccaggct atgcatgaat tcaccgatac ccgcgatgat
    14401 agtacccttg atgaaatctg gctggtcgag cactatccgg tattcaccca aggtcaggca
    14461 ggaaaagcgg agcacatttt aatgccgggt gatattccgg tgatccagag cgatcgcggt
    14521 gggcaggtga cttatcacgg gccggggcaa caggtgatgt atgtgttgct taacctgaaa
    14581 cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg agcaaacagt ggtgaatacc
    14641 ctggctgaac tgggtataga agcgcatcct cgggctgacg cgccaggtgt ctatgttggg
    14701 gaaaagaaaa tttgctcact gggtttacgt attcgacgcg gttgttcatt ccacggtctg
    14761 gcattaaacg tcaatatgga tctttcacca tttttacgta ttaatccttg tgggtatgcc
    14821 ggaatggaaa tggctaaaat atcacaatgg aaacccgaag cgacgactaa taatattgct
    14881 ccacgtttac tggaaaatat tttagcgcta ctaaacaatc cggacttcga atatattacc
    14941 gcttaattcc atacatcaat ggcccaattt acactgggtc attactcata atatctgcta
    15001 atgctttaca tctccttcgc gtttgtatat tttctttatc acttgcagaa atgcggtgcc
    15061 acgccttgct ccgtaactca ccccattgat taacctattg aaatagttga ttatggtgag
    15121 caaaagcgtt ccgttccaat tagacataag attattgtaa ttttttaaat attatttttc
    15181 catgaataaa attggagtaa gtgcgtggat agtaataatc aaattgaacc ctgtttaagc
    15241 agaaagtcgt cagaaggtaa accacaaata tttacaacct tacgaaatat tgatcttaac
    15301 cttctgacta tttttgaagc tgtatatgta cataaaggga tcgttaatgc agcgaaagtg
    15361 cttaatctga ccccctcggc aatcagtcag tctattcaga aactgcgcgt tatattccct
    15421 gacccattgt ttattcgcaa aggccagggt gtcactccta ccgcatttgc gatgcatcta
    15481 catgagtata tcagtcaggg ccttgagtcc attcttggcg cgctggatat cgaaggaagc
    15541 tatgataagc aacgaacgat aactattgcg accactccct cggtcggagc actggtcctt
    15601 cctgtcatct atcgggcgat taaaactcac tatccgcagc ttttactgcg caacccgccc
    15661 atcagcgacg cggaaaacca actcagtcag tttcaaaccg atctcatcat cgataacatg
    15721 ttttgcacca atcgtacggt gcaacatcat gttctgttca ccgacaatat ggtgttaatt
    15781 tgccgcgagg gaaatccact actctcttta gaagatgaca gagagactat cgacaacgct
    15841 gcgcatgtac ttctgttacc ggaagagcaa aatttcagcg gtctgcggca gagagttcaa
    15901 gaaatgtttc cggaccggca aatcaatttc accagctaca acattttgac aatcgctgca
    15961 ctggttgcca acagtgacat gttagcgatt attcccagcc gtttttataa cctgtttagc
    16021 cgctgctggc cgctggaaaa actgcctttt ccgtccttaa atgaggagca aatagatttc
    16081 tctatccact acaacaaatt cagcctgcgt gatcccatcc tgcacggagt aatagatgtt
    16141 atccgtaatg cattttgaaa aagtattgtt acagccagat cacagcgcag aacgataaaa
    16201 tggcacaatc caacaacaat tttacatttt cgcgaccgct ttggctgctt tcaggtccgt
    16261 ttcaatgata tactgccagt cgttaattca aaaatagttg ataattacaa caatctattg
    16321 aattgaaacg ctttccttcg taattcgcaa ctggaacacg cacgctatga gtaaacccat
    16381 tgtgatggaa cgcggtgtta aataccgcga tgccgataag atggccctta tcccggttaa
    16441 aaacgtggca acagagcgcg aagccctgct gcgcaagccg gaatggatga aaatcaagct
    16501 tccagcggac tctacacgta tccagggcat caaagccgca atgcgcaaaa atggcctgca
    16561 ttctgtctgc gaggaagcct cctgccctaa cctggcggaa tgcttcaacc acggcacagc
    16621 aacgtttatg atcctcggcg ctatttgtac ccgccgttgt ccgttctgtg atgttgccca
    16681 cggtcgcccg gtagctcctg atgccaatga accagtgaaa ctggcgcaga ccattgccga
    16741 tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt gatgacctgc gcgatggcgg
    16801 tgcccagcac tttgcggatt gcattactgc cattcgggaa aaaagcccgc aaatcaaaat
    16861 tgaaactctg gtgccggatt tccgcggtcg tatggatcgt gctctggata ttctgactgc
    16921 aacgccacca gatgtgttca accataacct ggaaaacgta ccgcgtattt accgtcaggt
    16981 acggcctggt gcagattaca actggtcgct gaagctgctg gaacgcttta aagaagcgca
    17041 tccggaaatc ccgaccaagt ctggtctgat ggtgggactg ggtgaaacca atgaagaaat
    17101 tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg atgttaacgc tggggcaata
    17161 tttgcagcca agccgccatc acctgccggt tcaacgttac gttagcccgg atgagttcga
    17221 cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat gctgcatgcg gtccgtttgt
    17281 ccgctcttct taccacgccg atttgcaggc gaaagggatg gaagttaagt aatcctgtaa
    17341 taattactta catctgtctg ataaaaaacc cgctttttga agcgggtttt ttgtatcgaa
    17401 caagatattg agggagcgtc ctgctcgcca cgtcactctt tatgagagag cttttcagcc
    17461 tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt catcattcat cgccttcttg
    17521 aaccctttaa tggccgctcc aaggtctccg cccagcgtac gtaacttctt agtcccaaac
    17581 agcagaacga ccagcgccgc aactaccagc agtttggtaa tactaatctc acccatagat
    17641 accttcttga catataaccg gcaacagaac gcgccttaaa aaccagagaa tattaacggt
    17701 cattcgacgc gcgcacacaa tagcaatctg taacaaggtg aatcaagcgc cgagtaaaaa
    17761 aacatcataa taattgcggc ggcgcaaagc gacggttgtt taagacgggc agttgcgcgc
    17821 gcacctgacg cacacgttcg ggcgtcactt ccgccataat gagtgcaggc atttctgacg
    17881 ctgccgcaat ggtgacgcca aagggatcta taatccggct ttgaccgata tttttgttcc
    17941 cgcactcccc cgccgccacc atataacagg tggtatccag cgcacgagcg gcaagcaacg
    18001 ttgaccagtg atgctctttg agcggcccgc gaacccaggc ggcaggaagt accaggattt
    18061 cagctccctg taatgcctgt gccagcgcca gctctggaaa gcgtaagtca taacaggtca
    18121 tcagaccgac cttcatccct tccacctcca gtaacggagc gatttcatta ccagcatcaa
    18181 cacggcgtga ttcctgaatg gcaaatgcat catagagatg cagtttggca taacgggcga
    18241 cgatgtttcc tgcctgaagt gccaccagca tattccatgc gcgccccggc gttgaaggaa
    18301 catgaatcgt cagaattgtc gtcatcatgt tacgtttact ttctcgccgt aaagtccgag
    18361 gaattcgcct tccagcagct gtgctgattt aaccgataga tctgcatcat gatcatcgcg
    18421 cgccagcaat gcttccggca ggacaaacag cgatacgtcg ttttccgccg cctgcgccat
    18481 caacgaggca caaatctcag cgttcttttc ccacacagat gtaacagcaa actgtcctgc
    18541 ggcaaccagc attgccacct cctgtcagag tcgtaaaatt cgccaacgac gattattcgg
    18601 cgttacactt atcactcata caaatcaaat agcaggattt tgcagtgtta caacttcttt
    18661 tagcagtttt tattggcggt ggtacgggaa gcgtggcgag atggctgtta agtatgcgat
    18721 ttaacccact gcatcaggcg attccgttgg ggacgctgac agcaaacctg attggggcat
    18781 tcatcatagg aataggattc gcatggttca gcaggatgac gaacattgat ccagtgtgga
    18841 aagtattaat caccaccgga ttttgtggcg gtctaacaac cttctcaaca ttttcggcag
    18901 aagtggtgtt tttgttacaa gagggccgct ttggctgggc attactgaac gttttcgtca
    18961 accttctggg gtcttttgcc atgaccgcac tggcattctg gctgttttcg gcctcaaccg
    19021 cacactaaag accaaaaaaa acccgctgat taagcgggtt ttgaattctt gctgacgtat
    19081 cttacagagc gattacgttt gcagcagaag ggcctttggc accgttagtg atttcgaact
    19141 ctacgcgctg accttcagca agagttttaa aaccattagt ctggattgca gagaagtgta
    19201 cgaacacgtc tttgctgccg tcttccggag taatgaaacc gaatcctttg gactcattaa
    19261 accacttaac gttaccttta atcttagaca tcaaaattac ctttacatga aaaatagaca
    19321 caaatgctgt gtcggttacc agtacaccaa ttgtggtacg ctttgtccag tgtaacttta
    19381 ctaaaaagtg ataaaagtcg cgtttttttt gatgttactt attttagcta ttgattttaa
    19441 agaagttact aaaacttcat ttgtctcaaa actgaaagcg catccaggca aagtacacat
    19501 tgccattgtt gtaggtaccc ggaatgtagg tcatctgaaa agtcactggg ccataaccca
    19561 cggaggccaa tggcagtaga accgggagag ggatgtaatt ccagttatcg cgtgccgtta
    19621 cgccagcggt gaatcccaga cctaaatgaa aattttcatc cgccagcggt cgccaggtac
    19681 tttcccatcc gtatccggca atcggttccc atttgttcca cgagtcctta aatgccatgg
    19741 catacaggcc atgccagttt cctttttcat cccaacgcga caggccaaaa ccgccacccc
    19801 acggtcgctc gttatagcga tcggtttttt ctttgtcgta agcgaaacgt gcatgccagg
    19861 tgatggcagg aatatataaa tcataatgtt caggctgttg ccaggtttgt gcaatatttt
    19921 ctctaaacgt tgtcatccac tcatctgcgt tagcaaaaac tttaccaacg ctgattaact
    19981 gaataaaaac aaaggaaaag atagcgacat atttactcac gttcatttgt gaccataaaa
    20041 catttatcaa aaatctacta ctagcatagc aaagctacaa ttaacataac cttaatagac
    20101 ccaacataaa gaataatctg aatagctgct ctatttattt ccgcttgttc aagttaaata
    20161 tttccgcgca tcgattaaag atgatgcgtc gttttctgcg atgggaatag tcaaaaaaga
    20221 aaaaccaagt cttttttgat gacaaaatgc aatcaaggaa aaattatttt attttttaac
    20281 gagatacaac aatcatctta acgaagtata taatatatat cgtcaaccta tgctcatatt
    20341 agattatttt ttgagcgcac agccgcagct aacaccattg caattaacaa atttgcatca
    20401 atccaccatc aatttgcaca cattattatg tgataattgc caaccgctaa atatgcgttt
    20461 tgttatctat gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta
    20521 atcagcattc atcgccaatt tattgggcat attttttcct taagctttag gaatttttta
    20581 ttatttactt tggggcctgg agacaggaaa aattatgctg acattcattg agctccttat
    20641 tggggttgtg gttattgtgg gtgtagctcg ctacatcatt aaagggtatt ccgccactgg
    20701 tgtgttattt gtcggtggcc tgttattgct gattatcagt gccattatgg ggcacaaagt
    20761 gttaccgtcc agccaggctt caacaggcta cagcgccacg gatatcgttg aatacgttaa
    20821 aatattacta atgagccgcg gcggcgacct cggcatgatg attatgatgc tgtgtggatt
    20881 tgccgcttac atgacccata tcggcgcgaa tgatatggtg gtcaagctgg cgtcaaaacc
    20941 attgcagtat attaactccc cttacctgct gatgattgcc gcctattttg tcgcctgtct
    21001 gatgtctctg gccgtctctt ccgcaaccgg tctgggtgtt ttgctgatgg caaccctatt
    21061 tccggtgatg gtaaacgttg gtatcagtcg tggcgcagct gctgccattt gtgcctcccc
    21121 ggcggcgatt attctcgcac cgacttcagg ggatgtggtg ctggcggcgc aagcttccga
    21181 aatgtcgctg attgacttcg ccttcaaaac gacgctgcct atctcaattg ctgcaattat
    21241 cggcatggcg atcgcccact tcttctggca acgttatctg gataaaaaag agcacatctc
    21301 tcatgaaatg ttagatgtca gtgaaatcac caccactgct cctgcgtttt atgccatttt
    21361 gccgttcacg ccgatcatcg gtgtactgat ttttgacggt aaatggggtc cgcaattaca
    21421 catcatcact attctggtga tttgtatgct gattgcctcc attctggagt tcctccgcag
    21481 ctttaatacc cagaaagttt tctctggtct ggaagtggct tatcgcggga tggcagatgc
    21541 gtttgctaac gtggtgatgc tgctggttgc cgctggggta ttcgctcagg ggcttagcac
    21601 catcggcttt attcaaagtc tgatttctat cgctacctcg tttggttcgg cgagtatcat
    21661 cctgatgctg gtattggtga ttctgacaat gctggcggca gtcacgaccg gttcaggcaa
    21721 tgcgccgttt tatgcgtttg ttgagatgat cccgaaactg gcgcactctt ccggcattaa
    21781 cccggcgtat ttgactatcc cgatgctgca ggcgtcaaac cttggccgta ccctttcgcc
    21841 cgtttctggc gtagtcgttg cggttgccgg gatggcgaag atctcgccgt ttgaagtcgt
    21901 aaaacgcacc tcggtaccgg tgcttgttgg tttggtgatt gttatcgttg ctacagagct
    21961 gatggtgcca ggaacggcag cagcggtcac aggcaagtaa atagtaatgc cggaggcaag
    22021 tttcctccgg ctttatcatg tcacccactg tggtatatgc gttgtggtct gccaactttg
    22081 ccgtgaacaa tttcagcaat aatcagatgg cggctggcgc aatattcaag ataacgcctg
    22141 gcagtggtgc ggctgatggt cagtgcctgc gccaccgttt ctgccgtatg ttgcacacca
    22201 ggctctttaa acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc ggtcggtagc
    22261 tcgtccttag gttcaccgcg agcataagca ttaaacatct catcaatttg cttctggctg
    22321 gcgctatcaa tactttccag catatgttta cgctggcgga aacgggttag cgtttgcccc
    22381 agccgttcat aggcaatggg cttaatgaga taatcaaata caccacaacg tacggcttca
    22441 gacaccgttt ccatatcgct ggctgcagtg gtaaacacca cgtcgccggg ataatgcgcc
    22501 tgcaccagtt catgcagtaa attaatccct ctaccgtcag gaagatagtt atcgagcaag
    22561 attagccccg gcttaaaacg ctcgatcatc attcgggcct gcgccagatt tcccgccagt
    22621 aatatctgac tgaatccggg aatgtgacga atatattccg catgcatctc tgccagcggc
    22681 gtttcgtcct caacgatcaa tagggttaat ggagctgtca ttaggtttca ctttcggaat
    22741 atagattgaa aataaggtac cgcagggatc attatcttcg agagtgataa caccaccgca
    22801 gcgcgttacg tagctggcaa tcaagtacaa cccaatgcca tgttcaccgg gctcgtcagc
    22861 acgcgtactg accccctgct caaatatttt gtctcgtaga gactctggaa cgccgcagcc
    22921 ctgatcggcg acttcaatca ccacatcatc gccttcatcg ctgaggaata attcaacgat
    22981 cttgtttcct tcatcgctac gcaggctggc ttcgaaggcg ttatcaagta aattgcccac
    23041 aatggctgca aactcggtgc tatccagtcc tggcggcagt tgcgaaagct ggctaccggg
    23101 gacaatgatc atttttagcc ccagttcccg ggcgcgctgc actttaccaa aaagcagccc
    23161 cgccacctgg cgatcggcaa acgcctcgcg caggctgtca ataagctgtt gctgggcctg
    23221 agactccccc tgcaccatcg ccagcacgcg atcatactct ttcatctgca acagaccatt
    23281 gagcgtcgac atccaattga gatgctcgtg tcgcaatgta cgaaggctct caacgtattg
    23341 ttttatttgc gtcagttgcg cattgagggt ggaaatttcg tctttactac gaaagctgat
    23401 aatggccccc agcaaatcat cacctgaacg aatagcttcc cggttggcaa taacgctcag
    23461 accgttaaag ttcgccacca catcctgacg tttttcatca atctgttcgg taaagaaatc
    23521 ggcgggcctg accacttcaa caatgggttt acccaaccat tgccgtccgg gggaactcag
    23581 ccccagcatc tttcttgcgt tacgattgat ggcggtaatg taaccatgcg gatccaccgc
    23641 aatcagccct tcataaaccg aactaaacag cgcctcttgc tgacggacca cgcgtgcgat
    23701 ttgctttggc tccatgccca tcatctgccg acggatatgc gcggccagga accacgacag
    23761 caacatcaga atccctaaca gcacgacaaa cacacctgcc atcggtaata aaaactcagc
    23821 ccgccagcta tcgattttac tcaccaggta gccaatcgac accacgccga tgacttttcc
    23881 atcgtcatca aagattggcg ttttggcgcg catcgccatc cccattgacc ctttcccggt
    23941 aatgaagtag ctctcccctt tctccagcgc gccctgtttg gtgaactgca taggataacc
    24001 aattttctcc ggattaggat ggtaaaggcg gatcgagtgc cggtccccaa tcaccacata
    24061 atcaaaatcg gtatctcttt gtaatttgtt agcgatggtc gccagccgtt tgtagtcacg
    24121 cgttttcacc gcagagatga cactgtcatt ggaggcaata attttcgcct gattcattgc
    24181 catgtcgcgt acatgaagcg ttaaatagtc ctcaaaactg gccgtaaaat attgcgccag
    24241 ggctgcaatg acaaatattg agaacaccag aatcagcaaa aagatacgca gcggaaatgc
    24301 cagtctttgg aaaaatgcaa actgtttatt ctcgttaagc tgcaacattg ttttccttgc
    24361 gggttgtccc gagcctcttt gacggatgta attaaaatgt tgaatcaaaa tacatagata
    24421 ttaattatga tcgtggcaaa ttttattcat taaatcaatt aaatcactta aacctgcgag
    24481 tcatttcgta attacattaa ttaaaaaact taaaatcatt aaataaataa aaaaccacta
    24541 actctatgtg aaataaatca aaatttcacg ccgaaatact ccttaggatg tatagcgaaa
    24601 ggagaaaaag atatacctcg atcaccccct ttctcccaag tgaaaataaa aggttatcag
    24661 tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt caggatacta
    24721 tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg
    24781 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac agtcgaagta
    24841 tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt
    24901 attaaatgcg ttgctatcag tgaatccgtc cgcggtgaag gactggcgct gacattagcc
    24961 actgaattga taaacctcgc ctatgagcgg cacagcacgc atctgtttat ttataccaaa
    25021 accgaatacg aggcgctgtt ccgccagtgc ggtttttcca cgctgaccag cgtacccggc
    25081 gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac gctatgccga atcgctgaaa
    25141 aaatttcgtc atccagggaa caagattggc tgcattgtga tgaacgccaa tccctttacg
    25201 aatggtcacc gttatctgat tcaacaggct gcggcacagt gcgactggtt gcatctgttt
    25261 ttagtcaaag aagattcttc acgcttcccc tatgaagacc ggctggattt ggtgttaaaa
    25321 ggcaccgccg atattccacg cctgactgtg catcgtggct ccgaatacat catctcccgc
    25381 gctacgttcc cttgctactt cattaaagaa cagagcgtca ttaaccattg ttacaccgaa
    25441 attgatctga agattttccg tcagtacctc gctcccgcgc tgggtgtaac tcaccgcttt
    25501 gtcggtactg aacccttttg tcgcgttacc gcccagtaca accaggatat gcgctactgg
    25561 ctggaaacgc cgactatctc cgcaccgccc atcgaactgg ttgaaattga gcggctgcgt
    25621 taccaggaga tgccgatatc cgcttcccgg gtacgtcaac tgctggcgaa aaacgatctc
    25681 acggctatcg cgccgctggt ccctgcagtc acgctgcatt atttgcagaa cctgcttgag
    25741 cactcccgcc aggacgcggc agctcgtcaa aagacccccg catgagaaac aggtgaaaaa
    25801 tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc tggggatgtg atgatacgca
    25861 tcgccccact cgatacgcag gatatcgacc tgcaaatcaa tagcagcgtt gagaaacagt
    25921 ttggcgatgc aattcgcacc accattctgg acgttctcgc ccgctacaac gtgcgcggcg
    25981 tacagctgaa tgtcgatgac aaaggcgcac tggactgcat tttacgtgca cgactggaag
    26041 ccctgctggc acgcgccagc ggtatcccgg ctctgccatg ggaggattgc caatgatttc
    26101 cgcttcgctg caacaacgta aaactcgcac ccgccgcagc atgttgtttg tgcctggtgc
    26161 caatgccgcg atggtcagca actccttcat ctacccggct gatgccctga tgtttgacct
    26221 cgaagactcc gtagcattgc gtgaaaaaga caccgcccgc cgcatggttt accacgcgct
    26281 gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt gtcaacgcgc tggattccga
    26341 atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc
    26401 gaaaaccgat accgctcagg atgttctgga tattgaaaaa gagatcctgc gtatcgaaaa
    26461 agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg gcgattgaat ctccgctggg
    26521 gattacccgc gcagtggaaa tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg
    26581 tgcagaagac tatgtgcgca acctgcgtac agaacgctcc ccggaaggaa ctgaactgct
    26641 gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac
    26701 cgtctattcc gacgctaaca acgaagccgg atttctgcaa gaagccgccc acatcaaaca
    26761 gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag attgatctgc tgcacaacct
    26821 ctacgcaccg acccagaaag aagtggatca cgcccgccgc gtcgtagaag ccgctgaagc
    26881 cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc aagatggtgg acgctccggt
    26941 tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt tccggcatcc gcgaagaata
    27001 aggcaatcaa aatgacgcag aaaattgaac aatctcaacg acaagaacgg gtagcggcct
    27061 ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa ctcgccaaag caaacctacc
    27121 aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct
    27181 ctggtttaca ggacggcatg acggtttcct tccatcacgc tttccgtggc ggtgacctga
    27241 ccgtcaatat ggtgatggac gtcatcgcga agatgggctt taaaaacctg accctggcgt
    27301 ccagctccct gagtgattgc catgcgccgc tggtagaaca cattcgccag ggcgtggtta
    27361 cccgcattta tacctccggc ctgcgtggtc cactggcgga agagatctcc cgtggtctgc
    27421 tggcagaacc ggtgcagatc cactctcacg gcggtcgtgt gcatctggta cagagcggcg
    27481 aactgaatat cgacgtggct ttcctcggcg tcccgtcctg tgatgaattc ggtaatgcca
    27541 acggctacac cggtaaagcc tgctgcggct ccctcggcta tgcaatggtt gatgccgaca
    27601 acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc ttatccgcat aatccggcaa
    27661 gcattgagca agatcaggtt gatttgatcg tcaaagttga ccgcgttggc gatgctgcaa
    27721 aaatcggcgc tggcgcgacc cgtatgacca ctaacccgcg cgaactgctt attgcccgta
    27781 gcgctgcgga tgtgattgtc aactctggct acttcaaaga aggtttctcc atgcaaaccg
    27841 gcaccggcgg cgcatcgctg gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg
    27901 atattcgcgc cgacttcgcc cttggcggta ttaccgcgac gatggttgac ctgcacgaaa
    27961 aaggtctgat ccgcaaactg ctggatgtgc agagctttga cagccatgct gcgcaatcgc
    28021 tggcccgtaa ccccaatcac atcgaaatca gcgccaacca gtacgctaac tggggttcga
    28081 aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag cgcgctggaa attgacaccc
    28141 agttcaacgt taacgtgctg accggctctg acggcgtact gcgtggtgct tccggtggtc
    28201 actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt cgcgccgctg gtacgcggtc
    28261 gtattccgac tctggtggat aacgtactga cctgcatcac cccaggctcc agtgtcgata
    28321 ttctggtcac agaccacggt atcgcagtta acccggcacg tccggaactg gcagaacgtc
    28381 tgcaggaagc gggcattaaa gtggtttcca ttgagtggct gcgcgaacgt gcgcgtctgc
    28441 tgaccggtga accacagccg attgaattca cagaccgcgt cgttgccgtt gtgcgttacc
    28501 gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga ataagccatg cacctgcttc
    28561 ctgaactcgc cagccaccat gcggtatcaa ttcccgagct gctcgtcagc cgggatgaaa
    28621 ggcaagcacg gcaacacgtc tggctcaagc gccatcctgt tccactggtc tcctttaccg
    28681 tggttgcgcc tgggccgatt aaagacagcg aggtcacacg ccgaattttt aatcatggcg
    28741 tgacagcctt gcgtgcctta gccgcaaaac agggctggca aattcaggag caggctgcac
    28801 tggtttccgc cagcgggccg gagggcatgt tgagcattgc cgccccggct cgcgacctca
    28861 agctcgccac cattgagctt gaacatagtc atcctctcgg gcggttatgg gatatcgatg
    28921 tcctgacgcc cgaaggcgaa attctctccc gccgcgacta ttcactgccg cctcgccgct
    28981 gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg aaaaacccat caactgaccg
    29041 atttactcaa ccgcatggag gcactgctga acgatgtcga tgcctgcaac gtcaactaaa
    29101 accacaaagc ttgcgacgtc attaatcgat gagtacgccc tgctgggctg gcgcgccatg
    29161 ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg
    29221 cacaaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca gggctggcta
    29281 ccccgtttca ttgaatttgg tgcctatagt gcggaaatgg caccagaagc ggtactccac
    29341 ggattacgcc caattggtat ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta
    29401 aacacgcata aaggcagcat tttttcttta gggctgctat gtgcggcaat tggccgtttg
    29461 cttcaactca accaaccggt aacgccaaca accgtttgtt ctacggcggc aagtttctgc
    29521 cgtggcctga ccgatcgcga actgcgtacc aataattcac aactgacggc aggtcaacgg
    29581 ttgtaccaac agcttggcct taccggcgca cgcggtgaag ccgaagcggg ttatccactg
    29641 gtgatcaatc acgccttgcc gcattacctc actctgctgg atcaggggtt agatcctgaa
    29701 ctggcattgc tcgatacctt gctcctactg atggcgatca acggcgatac caacgttgca
    29761 tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa
    29821 aaagggggca ttcgaacccc cgccgatctc gattatctcc ggcagttcga cagggagtgt
    29881 atcgaacgaa atctcagtcc aggcggcagt gctgacctac tgatccttac ctggttttta
    29941 gcacagattt aattatttaa gcacttgata aatttggaaa tattaatttt cggagaaccc
    30001 gtatgtcttt agcaaaagat aatatatgga aactattggc cccactggtg gtgatgggtg
    30061 tcatgtttct tatccctgtc cccgacggta tgccgccgca ggcatggcat tacttcgctg
    30121 tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat tccggcaaca gcgatcagtt
    30181 ttattgcggt tactatttgc gttattggca gtaattacct gctctttgat gccaaagaat
    30241 tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggcctg gctggttttt
    30301 ccagcaccac ggtatggctg gtatttggcg catttatttt tgcattaggg tatgaagttt
    30361 ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt catgggcaaa cgcacgctga
    30421 cgttgggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca ccttccaaca
    30481 ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg ttgtttaaat
    30541 cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca
    30601 ttagtaccag tctgagttcg tccatgtttg tcaccggtgc ggcaccaaac gtgctgggtc
    30661 tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg ttcctctgct
    30721 tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca
    30781 aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa ctgaaaacca
    30841 tgggtgcgct gacacgcaga gagtggacac tgattggcct tgtattgctc agcttaggtt
    30901 tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt tggtctgctg gcagtttcgc
    30961 taatgctggc cctgcacgtt gtgccttgga aagacattac ccgctataac agcgcatgga
    31021 acacactggt caacctggca actctggttg tgatggctaa cggcctgact cgttctggtt
    31081 ttattgactg gttcgccggt accatgagta cgcacctgga aggattctca ccaaacgcaa
    31141 cggtgattgt actggttctg gtgttctact ttgcacacta cctgtttgcc agcctgtctg
    31201 cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaaggtatt ccgggcgtac
    31261 caatggaaca actgtgtatc ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc
    31321 cgtatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt
    31381 actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgttgctg ttggttggct
    31441 ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc
    31501 cgccaggtaa gcccacctgg cgggcttttt tatgatttaa tagatagtat tcagtgtatg
    31561 tatgagttcc acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc
    31621 tctgcttccc ttctcttctg ccaacgcctt agcgttgcag gcaaaacagt atggcgattt
    31681 tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg
    31741 aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt
    31801 tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga
    31861 tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc
    31921 gcgcgccagc cgaatgtgtt catcgccgga aaccggatta tcactggaaa cggccgctaa
    31981 actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc
    32041 caaacacggt gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct
    32101 gaatcaagaa attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac
    32161 agtgagccgc cgtgactttg acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa
    32221 agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat
    32281 caaagctgac gccatcaacg ctccgctttc tgcaaactca ttcttgccac aacctcaccc
    32341 aggtaactgt ggcaaaacct ttgtgattga taaagcgggt tattaatcat ctgatttaca
    32401 aaacacgcta attcgtcttc cataaaccaa ggcgattaag atttatacat aatgcgctgc
    32461 attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc
    32521 atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg tatgtttatc
    32581 tgcgacactc gcagcaccga cgacatggag taaaaatgtc cagaccaact atcatcatta
    32641 acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat gctggtttgc
    32701 caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg ccagaagaga
    32761 tgccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt agcgatggcg
    32821 aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat actcagcttt
    32881 ccgttatggc tccggtaggt gccgcactac tggggctgcg cgttggcgat tccattcact
    32941 gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa taccagccag
    33001 aagctgctgg cgactacctg ctttaatccc atctgcacaa gcgataactc gctttgccca
    33061 gaggatgcac tcgcatcctc tttacgcaaa atcagcaatt tttcctggtt ctcactgttc
    33121 acttttgctg caaaattatt ccgtttcgtt tgtgggaata accatggcag aagcatttta
    33181 catcctgata ggatttctga ttatggcggc gattatcgtc atggctgtcc tttacctcga
    33241 aaaccactcc tgacgatcat attgttcccc ccggcatcgc attcaccaga cctgaaaccg
    33301 ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag aatcacctta cggcactcct
    33361 cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc ttcaaacggc atatagtggg
    33421 taacaatttc ttctggtttc agtaatcctt tctcaattaa cggtaataat tctcccagcc
    33481 atgcgtgaac gtgggtctgt cccattttaa acgacaaccc tttatcaaag gcgtcgccaa
    33541 acaggaaacc gtgaataaat ccagcgtaga cgcccggtac gctaacaatg ccgccacgcc
    33601 tgaccgccgc aatacactga cgcaacgctt taccgctgct gccctccagt ttcaggttag
    33661 tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc gacggcgtct attactgcat
    33721 ccacgccccg gtgacctgcc gtttgttcaa taattgactg tgccggatcg ctgtcttcat
    33781 caaaattaat cgggatcgcg ccgtagcggt cggcggcgaa atgcaagcgg tagggatgat
    33841 gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc acaggcgatt gtcaacaatc
    33901 ccacaggacc agcaccatag actgcaacgc ttgaaccttg ttggatctgc gcattttttg
    33961 ctgcctgcca tgccgttggc agaatatcag aaaggaaaag cgctttatca tctgaaagca
    34021 aaggcggtac tttaaacggc cccacattcc ctttagggac gcggacatat tccgcctgcc
    34081 caccaggaac gccgccatac aggtgactat aaccaaacaa tgccgctgga gctggtatct
    34141 gttttttatt gagcgcagcg cctttacccg cattggtatt ttcgcaggcg gcatattgtt
    34201 gcaatcgaca gaaaaaacag tcgccacaag caatgacgaa cggaattacc actcggtcgc
    34261 ctttttgcaa attttttacg tcctttccgg tttcaactac ttcccccata aattcatgac
    34321 caaaaatatc gccatgttta acctgaggta ttttgcctcg ataaagatgg aggtcagagc
    34381 cacagatcgc cgttgccgta atacgcagaa taatatcatc tgcctgttca acgcccggat
    34441 cgggaacatt ttctacctga acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac
    34501 ctccagagac agttcaaaaa agagtcagtc tttaagggta gctatcacag cgggaaaacg
    34561 ggcggtacca caggttttgg gagaattcct ggtgttcagt gagaatatat tcatcacaga
    34621 aacagttcgt aaggaaaaag aaccagacaa atatcaggga taaatattat aatcatggcg
    34681 aatgatttgt tcatgattaa cagggagaaa gggttatgta taagacaatc attatgccag
    34741 ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc
    34801 aggatgacgg agttattcat ctacttcacg tactacccgg gtcagccagc ctgagcctgc
    34861 accgttttgc cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa gcacaagaac
    34921 gtctgcaaac gatggtcagc cacttcacca tcgatccttc ccgcattaaa caacatgtcc
    34981 gttttggtag cgtgcgggat gaagtcaatg agttggcaga agaactgggg gctgatgttg
    35041 tagttattgg ttctcgcaac ccatcgattt cgacccatct gttaggttct aacgcctcga
    35101 gcgtgatccg ccacgccaat ctgccggtgc tggttgtgcg ttaattgcca gaataagtat
    35161 cccgccctgc ccggagccat cccggcgggg ctttttaatg ccataaaaaa gccgccaggt
    35221 ttgaccccgg cggctaagca attgcaggtg aatcttactt cttcttatgc agttttggtg
    35281 cgaatcaggt agtcaaaagc actcagagag gctttggcac cttcgccagt ggcgatgatg
    35341 atctgcttgt acggaaccgt cgtacagtca cccgctgcga acacgccttt cacgttggtt
    35401 tcgcatttcg catcaatgat aatctcgccc atgcggttac gttcgactgc gccttcgagc
    35461 cagttggtgt tcggcagcag accaatctgg acgaaaatac cggccagttc gatgttgtga
    35521 atatcgccgc tgacacgatc tcgatattcc agaccaacga ctttgctgcc gtcgcctttc
    35581 acttccgtgg tttgcgcatt cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc
    35641 tgcagaacct ggtcggcttt catttctggc gcaaattcca gcagcgttac gtgctcaacg
    35701 atacccgcca ggtcaattgc cgcttccacg ccggagttac cgccgccgat aaccgctacg
    35761 cgtttacctt taaacagcgg gccgtcgcag tgcgggcagt aggtcacgcc tttggtgcga
    35821 tactgatctt cgcccggaac gttcatgttg cgccattttg caccggtcgc cacgataatg
    35881 ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct gatgcagacc accttcaact
    35941 gctgctggga tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc gtattcatca
    36001 acgtgaactt tcagtgcgcc tgccagcttc tgcccttcag tcttcggtac agaaatgtag
    36061 ttttcgatat caacggtatc gaggatctga ccaccaaaac gttcgcccat cagaccggta
    36121 cggatgcctt tacgtgcgga gtaaattgct gccgctgcac ccgccgggcc ggaaccgacg
    36181 attaatacgt cataagcatc acgcttgttc agctcttctg ccgcacgttt ttccgcgcca
    36241 gtatcaattt tggcaacgat ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg
    36301 tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg
    36361 ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcagta cgctcatcag gttcagcgcc
    36421 tgcaccacgt ccgggcagtt gtggcaagag agcgagtaat aggtttcgaa ttcaaaatca
    36481 ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt ctttcgacgg atgaccaccg
    36541 gtccacagca acgccagtac cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa
    36601 cgtggcccct ggttggaacc tgggttggtg atcaggaaag acggcttacg caccggcaag
    36661 ctgttatctt ctttaaaggt gactttgtct gacagttctg cgatttcagc caacagttcc
    36721 ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc
    36781 aatttctcaa ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat gtttatctcc
    36841 tgggctttaa acatcatcat gcaagcggcc tctgcggcta cctggatgca gcttgcatca
    36901 tggtgcgggg aagaaaatcg ggtgccgcag cacccgaaga attaggtgaa tttccgaagt
    36961 atttcgcgtc agagcaaggc ggctagcctg tgaatccccg ggagcttaca caagtaagtg
    37021 accggggtga gcgggcgacg ccaacgccgc tatggcgtga aagacgaagg aaatttagat
    37081 tttaccaacc aggtccagag acggagccag agttgcttca ccttctttcc atttagccgg
    37141 gcaaacttca cctgggtgag aagctacgta ctgtgctgct ttgattttac gcagcaggtc
    37201 agacgcgtca cggccaatgc cttcagcggt aacttcgatt gcctggatga taccctgcgg
    37261 gtcaacaacg aaggtcgcac ggtcagccag accttcatct tcacgcatgt tgtcgaagtt
    37321 acgggtcagg gcgccagtcg ggtcgccgat catcgcatat ttgattttag cgatggtttc
    37381 agagctgctg tgccatgctt tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc
    37441 tacgcccagt ttctgcagtt cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca
    37501 tacgaaagta aagtcagccg ggtagaagaa gaagacgctc cagcggcctt cggtatcttt
    37561 ttcggtgatt tcgatgaatt cgccgttttt gaatgcctgg tttttaaaag gtttaatttt
    37621 ggtgttaatc aaggacatct atacttcctc cgtgttttcg atgagatgta aggtaaccaa
    37681 tttttgccga ttcggctaat gcgtttccat tatcaaatcg ataagtttta ccttacaacc
    37741 ttcgtaagac aactttgtga actttgcacc gccagcggcg ataaaaagta aaatggcccc
    37801 ctttccaggt gaccgtgtaa tctgaactac cctttggtat cttcagtgcc tgcctgcagg
    37861 ctgaggtgtt gcccttacaa atgcaacaac gacatggatt acaacaccct cataaacaaa
    37921 gggcaatcac ctgatctaag ctcttaccta tgacagtgat aggttatgcc ttttactcga
    37981 cttttgcact gactgaaaag gacaaattaa tgttaaaaaa gatactttta ctggctctgc
    38041 ttcctgcaat cgtcttcgca gaggaacttc ctgctccagt aaaagcgatt gaaaaacagg
    38101 gcattacaat catcaaaaca ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt
    38161 atcaggatat gggcgtcacc atctacctga ctccagatgg taagcacgct atctctggtt
    38221 acatgtacaa cgagaaaggt gaaaacctga gtaacacact tatcgaaaaa gaaatttacg
    38281 caccagccgg acgcgaaatg tggcaacgga tggaacaatc ccactggctc ctcgacggta
    38341 aaaaagatgc gccggtcatt gtctacgtct tcgccgatcc gttctgccca tattgtaaac
    38401 agttctggca gcaggcgcgc ccgtgggtag attctggcaa agtgcaatta agaacattgt
    38461 tggttggggt tatcaagcca gaaagcccgg cgacagcagc ggcaattctt gcctccaaag
    38521 atcccgcaaa aacctggcaa caatatgaag cctctggtgg caagcttaag ctaaacgtgc
    38581 ctgcaaacgt aagtacagag caaatgaaag tgttaagtga caatgagaaa ctgatggacg
    38641 atctgggggc aaatgtcacg ccggctatct attacatgag taaggaaaat acgctacaac
    38701 aggccgtggg gttgcccgat cagaaaacgc ttaatatcat tatggggaat aaataaaagg
    38761 taattcatgg atggttgaac tatatccctg cttttcaacc atcctaaaaa agcatgaatt
    38821 gctcgctctg ccctaagtgg ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa
    38881 aaaacataat caataaaaat atatacgcaa aaaacattga ttaagtgaat atatcatgga
    38941 agaaaaatat aaccggagta gtgtatggcc aatctctacg acttgaaaaa gttcgactta
    39001 aatcttcttg tcatatttga gtgtatttac cagcatttaa gcattagcaa agctgctgaa
    39061 tcactatata tcaccccatc agccgtgagc cagtcgttac aacgcttacg tgcgcaattt
    39121 aatgaccctt tattcattcg ctcaggaaaa ggtatcgcgc cgacgacaac agggttaaat
    39181 ctgcatcacc atcttgagaa aaatctcaga ggccttgaac aaacaattaa tatagtcaat
    39241 aagtccgaac taaagaaaaa ctttattatc tacggccctc agctcatttc ttgctctaat
    39301 aatagtatgc tgatccgctg cctacggcaa gattcctctg tcgagattga gtgccatgat
    39361 atcctcatgt cagcagagaa tgctgaagaa ctgttggttc accgcaaagc cgatctggtg
    39421 atcacccaaa tgccagtgat cagccgttcc gtcatttgca tgccattgca tacgattcgc
    39481 aatactctaa tttgtagtaa taggcatcca cgaattactg acaatagcac ttatgaacaa
    39541 attatggcag aagagttcac ccaattaata tctaaatcag caggggttga tgatattcaa
    39601 atggaaatag acgagagatt tatgaatcgc aaaatctcat ttcgtggttc ctctttgctg
    39661 acaatcatta atagcattgc agttacagat ttactgggaa tagtccccta tgaactttat
    39721 aattcctacc gtgactttct taacttaaaa gagataaaac tagaacatcc gttaccttca
    39781 ataaagcttt acatttccta taataaatct tctttaaata atttggtgtt ctcccgattt
    39841 attgaccgtc taaatgagag tttttgatat ataactacag acagctacta taactttcat
    39901 ctatttattc acagcgcgaa tgaaaaataa aacactctgc tttacagtgt ttactttata
    39961 attaacagac ttatcttact gatcaccctg taacgttcag agagcgtctt cagatgtcta
    40021 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
    40081 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaagac tctggtttaa
    40141 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
    40201 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
    40261 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
    40321 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtacgtcaac
    40381 ctccccaaga tgcgataacc gaccctgact ttttctcctt ttaccagcca ggcatgacct
    40441 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gcggcgatga
    40501 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
    40561 aacaacgttt tgccgacgat aaaccctgga ctacggccgc accaggcggt catagctggt
    40621 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
    40681 aaagtctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
    40741 tgcgaatttg cgaacctttt ggcccggagc aacgacaagg attatgactc tatcacgtta
    40801 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
    40861 tttacgccgg acatgacaat catttctatg ggcatcggaa aatcctcaag ccagaacatt
    40921 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
    40981 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
    41041 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
    41101 aagttttact caataacgat tactggtgtc gggcattatc atttagcccc acaaaatcga
    41161 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
    41221 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
    41281 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
    41341 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
    41401 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
    41461 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
    41521 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
    41581 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
    41641 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
    41701 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
    41761 gcgcctgaag caaattaacg gcctgcaaga gttgtttgcc gaccgtcaat attcccgcgc
    41821 ctggacggtt aaatagctaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
    41881 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
    41941 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
    42001 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
    42061 tctccagccg gctttcattt aaagcattca ccagaatatc gcgcttctgg cgataaaagt
    42121 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
    42181 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
    42241 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
    42301 aagaaaccgc cactgcccgc tcacgcagct gcggatgcgc cagcacactg gcatggccct
    42361 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
    42421 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggggttat
    42481 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
    42541 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
    42601 cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
    42661 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
    42721 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
    42781 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgtgca acgtggtgcg
    42841 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
    42901 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
    42961 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
    43021 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
    43081 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
    43141 ggctaactac ttttcacatc caggaagttt caatcacctg cacgattttt tcactgatga
    43201 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
    43261 acttccgcca gcgtttggac tgccaggggc aaagcatatt ttgtttcgcg gtcattgcag
    43321 cgaaagcgat gtacaacaac tggcggctga gtccggtgac gaccgcagcg tggtgattgg
    43381 cgtcggtggc ggtgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
    43441 gtttgttgcc gttccgacga tcgccgccac ctgcgccgcc tggacaccgc tctccgtctg
    43501 gtataatgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
    43561 gctggtggaa ccggagatta tcctcaatgc accgcaacaa tatctgctgg cggggatcgg
    43621 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
    43681 gctaaccgtg cgactgggga tcaataatgc gcaagccatt cgcgacgtct tgttaaacag
    43741 tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt
    43801 ggtggatgct attattgctg gtggtgggat ggttggtggt ctgggcgatc gttttacgcg
    43861 tgtggcggca gctcatgccg tgcataacgg tctgaccgtg ctgccgcaaa ccgagaagtt
    43921 tctccacggc accaaagtcg cctacggaat tctggtgcaa agcgccttgc tgggtcagga
    43981 tgatgtgctg gcgcaattaa ctggagcgta tcagcgtttt catctgccga ctacactggc
    44041 ggagctggaa gtggatatca ataatcaggc ggagatcgac aaagtgattg cccacaccct
    44101 gcgtccggtg gagtccattc attacctgcc agtcacgctg acaccagata cgttgcgtgc
    44161 agcgttcaaa aaagtggaat cgtttaaagc ctgacgtaca aatttagcag cagcgcgcgc
    44221 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg
    44281 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
    44341 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
    44401 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc
    44461 cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccaccgg ggagagggtt
    44521 agggtgagag ggaaccctgg gcactaagcc aacattcgcc aacatccccc cctcactctg
    44581 actttagtgt gcgccttttg cctgcgccac gatctcctcg acattttccg gcattggttc
    44641 atacggcgtt tctttcgccg ttggcttcgg atctttcaat gccgcaagtg ccgtcttaat
    44701 cgagaacagt gccagaacca ccacgaccac cataaagaag atggttaacc cggcatccag
    44761 acggttgttg aacaccagtt gcgccagctg tgactcagta tactgcgacg gaatattgcc
    44821 gctgtcgatc attgcctgga acttattagc aatggccagg aagccgactt tcgcatccgg
    44881 gctaaacgct ttctgccagc ctgcggtcag ggtacaaatc agcagccagg ccgttggtac
    44941 cagcgccacc caggcgtaac gttgacgttt catcttgaac aacaccacgg cacagagcat
    45001 cagcgccatc cctgccagca tctggttggc aataccaaac agcggccaca gagtgttaat
    45061 gccgcccagc ggatcgacca cgccctgatg gaggaagtag ccccacgcca gtacgcacag
    45121 cgctgttgcc agcaggttag caggcagtga atcggtccgc ttcaggccag gagacaccac
    45181 gcccagcaga tcctgcaaca taaagcgcgc agcacgcgta cctgcatcca ccgccgtcag
    45241 aataaacagt gcttcaaaca aaatggcgaa gtgataccag aacgccacat ccatcatgcc
    45301 gcccagcgcg ccgtgcagaa tgtaggccat ccccaccgcc agcgtcggcg caccgcccgc
    45361 acgggaaatg atcgactgtt cacccacttc gctggcaatc tggtttagcg tatctggtgt
    45421 aatgctaaag ccccagctac tcaccacctg cgcggcagaa gcgaccacat ccgccgtccc
    45481 tgccggagcc agcaccgcca tcgggctgtt catggcaaaa tacacgcccg gatcgatgat
    45541 acaggcggaa accagcgcca taatcgccac gaaggattcc attaacatcc caccgtagcc
    45601 gataaagcac gcctgccctt cgttcgccag catcttcggc gtggtcccag aagagatcag
    45661 cgcatggaag ccagacaccg cgccacaggc gatggtgata aacaggaacg ggaacaggtt
    45721 accggtccat accgggccag tgccatcgac aaatttggtc agcgcaggca tggtcagcgt
    45781 cgggcgcata atcaaaatgc ctaccgccag accgacgata gtcccgattt tcaggaaggt
    45841 agagaggtag tcacgcgggg ccagcagtaa ccacaccggc agcaccgccg ccacaaaacc
    45901 gtaacccacc agcatccagg tcaactgcac gccggtaaag tcaaagtacg gtgcccaggt
    45961 cggactttct gccacccagc cgccagagat aatggcgaaa atcaggaata ccagaccgat
    46021 gaccgacact tcgccaatac gccccggacg cagatagcgc aggtagatcc ccataaacag
    46081 cgccagcgga atggtgaacg caacggtata tgttccccac gggctatgag tcagggcttt
    46141 caccacgatc atcgccagca ctgcaaggat aatgaccatg atcataaagc aggccaccag
    46201 cgcaatcacc ccggcggttg gccccatctc ttctttgacc agctcaccca gcgagcgacc
    46261 gtcacggcgc gtagaaacaa acagcaccat gaaatcctgc accgcaccgg cgagaaccac
    46321 cccagcgagc agccagatca tccccggcag gtagcccatt tgcgccgcca gtaccggccc
    46381 caccagcgga cctgctccgg caatggccgc aaaatggtga ccgaacagca ctttcttgtc
    46441 cgtcggcaca tagtccagcc cgtcgttatg gcgcaccgct ggcgtcatac gcgtcgggtc
    46501 aaccgccagc acatttttgg cgatatacag cccataaaaa cggtaagcga tcagatagat
    46561 acagaccgac gccaccacaa tccacagcgc gttgatctgt tccccacgat ttaaagcaat
    46621 gtatcccaga gcaaatgctc ccattacaga gagcactgtc cagacgaggt atttccctga
    46681 tttgttcata gttgttatcc gtgtgcgtgt ccatagagat gttacatttt gtttctataa
    46741 catctttgta caatttaaca acacagcaac catgtaaatc ggagttgaaa ccaatattta
    46801 acttaacaat gttaactcga tcactccgtt aaccgagttt tcctgcaatc tcatctaatt
    46861 ctgtcgggtt catcccaaaa ctgccgtacc cagccgacaa gtgcagcaac gccgcccctg
    46921 ttcatcgaaa acgacgattt cccagctctg attttgccga ccaagatgca gcggctggca
    46981 gacgccgcgt acctttccct cagacaccgg gcgatggtgt gttgcattaa gttctgtgcc
    47041 taccacacac tgtccgtcgc gggtcatcat aaatccggcc atcgatccca gcgtttccgc
    47101 cagcgccgcc gacgcgccgc catgtagtaa accgaacggc tgatgagtac gggtatcaac
    47161 cggcatttcg gcttccagca caccatcgcc cagacgggta tacacaattc ccagatgcgc
    47221 caccattgtg ttatcgctgg tggcgttcag ttcgtcgagc gttaaatggc gtttccagat
    47281 catgcttatg cccccagcgt tgagccgcca tcgaccacaa tatcctgtag ggtaatatgg
    47341 ctggcgaggt cagaggcgag gaacaaaatc gtgttggcga tctcttgtgg acgggcgatt
    47401 ttccccagcg gaatgccgag tttaaactgc tcgccaaagc cgcgaatacg ctgttcttcg
    47461 gcgtcatcgc tcacccacag cgtgcgttgc atatcggtgt cggtggagcc aggcgaaacc
    47521 acattacagc gcacgccgct acccgccagt tccagcccga cgctcaacgc caggcttttc
    47581 agcgccgctt tcgatgcgcc ataagcactc atgccaatac gcggcgtgtg cgcggcgtcg
    47641 gacgccacag tgacaatcgc cccgccccgc tgacggcgaa actggttcat ggtttgctgg
    47701 aacaggttaa acgcaccgcc gacgttaacc gcaaaagtct gctgccagtc ctctttactg
    47761 agctgatcgg tcgcgcccat gcgtaaaatt cccgccgcat tgaccagcgc gtccagtcgc
    47821 tccgtttcag ctaacagtcg ctgacacact tgcgcgacct gcgcagcgtc ggcaacatcc
    47881 atcacttcgg tcgcaaaggg atattgctcc tgagtgaacg cttgatcaaa acctgtaact
    47941 ttcgctcccg cctcaacaaa cgccagcgcc gtggcgtagc cgataccttt acctgcgccg
    48001 gttacccaga catttttacc gctgaaatcc attatttcac ctcgcgggag agtagcttcc
    48061 accaggcgtc gatggtcggg tttttcgcca gcatgacaaa gtcgatatca ccatgcactt
    48121 tgcgccagcg cgccgccagc gccatcatgc gcaccgaatc cagaccgtag tcgatcaggt
    48181 tgtcgtcatc gaacggttca tcggactcgt ccagcaacgg caggatcacc tcacgcagcg
    48241 ccgctttgct ggcggggata ggtgctggca gtaattcttc agtcatcacc acccggccag
    48301 aacgtccggc cacatatttc agcgacatca aatgctcgtc acggctgaaa tcggccagcg
    48361 catccgccac cataaacggt ttaatatcgc gcataaatgc gtcggttgcg gtggtcatac
    48421 agccaatgtg ggcatatacc ccggtaataa tcagctggtt acgtccactc tctttcagca
    48481 tttgctccag cggagaacga tgaaacgcgc tgtagcgcca cttcaccagc accgtgtcgt
    48541 cggcatctgg cgtcaggcga tccaccacct tttgctgttc cggcgagcgg gtcaggcccg
    48601 gcccccacat atcattcaac agcgcccgat cttcatcgct ctgctctttc ggctgggcgg
    48661 tgtaataaac cgggatattg tgctgtttgc agtagtcgcg cagcgcagca atattcgcga
    48721 tcacctgctc catcatcggg cagttctcgc cccagaagct gacaaaatag tcctgcatat
    48781 catggattaa caacgcggca cgttgcggtt caaaggccca gtcaacttta ttctgcggaa
    48841 tatcgtgaga ctccggcagt gcgtaagcct gtaattttgg aatagccatc gtgttctctc
    48901 cttcaggctg atgcgcgtga cgccagccac tgacgtaatt gttttttatc gactttcccg
    48961 accgccgtca gcggaagtga atccacacac tccacgcgat ccggtaattt aaattcggca
    49021 ataccctgtt cacgcaggaa acgacgcacc tgcaccgcgc gcagcggctc ttttaccacc
    49081 agataagcgc agcttttttc gcccatcagc tcatcttcca tgctcaccag tgcggcgtag
    49141 atcaccgccg ggtggcgcag cagcaggttt tcgatctctt cggcagcgat cttctcgccg
    49201 ccacggttaa tctgatcttt ctcgcgcccc tgcacggtga tgtaaccctc tggatcaata
    49261 gagatcagat cgccggaaca gtaaaaaccg ttggcatcaa aggcgctggc attgtgctgt
    49321 ggacttttgt aatagccgcg gaaggtgtac ggcccgcgcg tcatcaggcg tccgacttcc
    49381 ccttgcggca gtggatttcc ttcggcatcg gcaacccata cttcgtcatc cggacacatt
    49441 gggtaaccct gggtatggat aattttctcc gcgctatcat caagtcgggt gtagttcacc
    49501 agcccttccg ccatgccaaa cacctgctgc aactgacagc caatctcagc gggaatacgc
    49561 gccgcaaggg tggcagaaag acgtgcgccg ccgacctgta acagtttcag cgaggcaagc
    49621 tgcgcccggc tttcgccttc gatcagcgcc tgcaaccaca ggctgactgc gggtggcacc
    49681 agcgcggtaa cgttaacctg atgtttttca atcaatggga aacagagcgt ggcgctggga
    49741 tcggccgcca gaacaaccgt tcctccggca agaaagacgc ccagcgatcc tggcgaactc
    49801 atggcgtagt tatgagccgc cgggatcgcg cacaggtagc gtgtctgttg tgtgaactga
    49861 caaatctcga cgctacgacg cacgctgtag tagtagtcgt tatgagtgcg cgggatcagt
    49921 ttcggtgtgc cggtggtgcc accggaaagc tggaaatagg ccacttcatc agcaggtgat
    49981 ggcgtggcag taaaatcctc agccggatgg ttaatcgcat cctgcaagtt atgctcaccg
    50041 ctgtcgttga gcagttgcac cacgcgaatg gaggaatgtt ctgtgacgaa agtattgagg
    50101 aaatcatccc cgctaaacag cgcatgttgg cgatcggcaa tcagcaatgc gggttcaatc
    50161 tggctggcat aggcgttcag ttcactacgc tgatggctga acaacgccag caccggcgca
    50221 acgcccagtt tcagcagcgc gaaaaaggta atatacaatt cagcgacgtt acccagttgt
    50281 accagcgcgg tttcaccagg tttaatgccc tgacggcgta aactacacgc gaggttatcc
    50341 gccgcctgat tcagctcccg ataactcaac tgtcgctcgc cgtcgataac cgcgatgctg
    50401 tcactcgcag catgtcgcgt cagaatgtcg gtcagcggca aatcctgcca gtagcctttt
    50461 tcccgatagc gacgggcaaa ctcttccggc cagcgggtga atggaatgct catcctcgct
    50521 ccttaatgca atccaaaaac gttcaacatg gtagaaagtt tgacgcctgt ttcgcgccac
    50581 tcacccaacg gtgacgacgc aggcacaatc cccgctccgg caaacagacg cacctgattt
    50641 tcccgcagct tcgcgcagcg gatggtcacc acccattcgc cgttaccttc gctgtcacac
    50701 caacccacaa tgccgccaaa cagttcgcgg tcgaacggtt ccagttcagc aataacctgg
    50761 gtcgcggcct gatgcgggaa accgctcagc gcgggggtcg gatgcagcag acaggccaga
    50821 gtcagtgcgt tttcttgcga attcgcttta ccttcaaagg gagttgcgag atgccacagc
    50881 gtcggcgtgg tgatcagctg tggagaagaa ggaacgtgta actcactact gcgttcgcgc
    50941 agtacctctt tcatcgcctg agtcaccagt tcatgttcat ggcgatcttt ttctgacgcc
    51001 agcagacgat tacctgcttc gcgatcgagc acttcatccg gctgacgacg cgcggaaccg
    51061 gctaacggaa tggagctaaa acgctcgccg tctttacgta gcagcagttc cgggctggcc
    51121 cccagcagga cgccaccatc agccagcgga acatggaagt tgtaactaac cgggttttgc
    51181 gcaatcaacc gttccagcaa tacgccacta tcaatggcgg cgtcagtggt gatatcaatc
    51241 aaccgtgaca acaccacttt gtcgacctgc ggcgtggcgg taagtgcggc ggcgcgggca
    51301 accatctgtt caaacgtggt ttgctccgga attgcctggc gttccaccac attcagcgac
    51361 tggctgcggg tgaaacggcg tgcggaagct tgtttttcct gacgggagaa cgactgccag
    51421 gattcaggaa tatacagcga cgaaggctga cgtggatcga agggaatcgc cccgaccatc
    51481 accggatttt tgatgccctg cgctttggca tcggcaaaca gcgcggcgag tttttgctgg
    51541 aagggactgt cgggcgaatc cccgttcaca gccggttcat cgaagcgggc gaaacatcct
    51601 gacgtcgtaa aactgcggta cggcgacata aagaaaaagc gattgggcgc aagtgttgcc
    51661 atggtctgct gtacttcctc agccagtgac gtatccatat catcctccac aaaatgataa
    51721 aggctttaat aatgattatc atttatattt tcggtcgcta acctaaaagc aaacgcgcac
    51781 tatgtcaact cttgaggtaa cgcaatctgc aactcggggt tgcatctggt tgtggttaca
    51841 atgaaaatga gaagcattat tgatggattc gcataagcgc aatgtgatgt cctgcgccgt
    51901 tctgccccct ctcccttcca gggtgagggc tggggtgagg gttaatgttc gcaccagtgc
    51961 tggctgttcc cctcacccta accctctccc caaaggggcg aggggacgga ttgtgcgctt
    52021 tgtcgaattt gtcattacgc ccttaacctt attaataaca ggaagctgat ttgtgagact
    52081 cgccccgctc taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgc
    52141 agttcaggcc gctgactggc cgcgtcagat tactgacagc cgtggcacac atacactgga
    52201 aagccagccg cagcgtattg tttccaccag cgtcaccctg accggctcac tgctggcgat
    52261 tgatgctccg gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cggatgacca
    52321 gggcttttta cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat
    52381 cggcgaaccg agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc
    52441 aaccggcggg gattcggcgc tggcactgta tgatcagctt tccaccatcg ccccgacatt
    52501 aatcatcaat tacgacgaca aaagctggca gtcgctgtta acgcaacttg gcgaaattac
    52561 cgggcatgag aaacaagcgg cagagcggat tgcgcagttt gataagcaac tggcggcggc
    52621 gaaagagcaa atcaaattac cgccgcagcc ggtcactgcc attgtctata ctgccgctgc
    52681 acacagtgcc aatctctgga cgccagaatc agcacaaggg cagatgctgg aacaactcgg
    52741 ctttacgctg gcgaagttac ccgcagggtt aaatgccagc caaagtcagg gtaaacgcca
    52801 tgacatcatt cagcttggtg gggaaaatct ggctgcaggg ttaaatggcg agtcactatt
    52861 cctgttcgcc ggtgatcaga aagatgccga tgctatttat gctaatccgc tgctcgcgca
    52921 cctgcctgca gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgtctgga
    52981 ttactacagc gccatgcaag tgctggatag gcttaaggcg ctgttttaag cattaactgt
    53041 cggacgctgt cacctgcggc ggcgtctggc gaaaatgtcg caactccacc agcaccagca
    53101 ataacaacac gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg
    53161 tcatcatcgc gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct
    53221 gcgccgtcca caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca
    53281 ttgtgtattg cagcaacgag ctgaccgcac tcaaccagcc gaacagcgcc agacaaacca
    53341 cgcctaaaat ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg
    53401 tggagagcag catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg
    53461 caccaatagc cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca
    53521 tctgccagtt gtcagccagc gccggataca gtacccgcac cgcgctcgcc atcgtcaata
    53581 aaccacccag cagcgcaatc ccgcccacca gcgggctggc aagcagaaaa cgaaatcctg
    53641 ccagtaatga tttcaacgga tgctcacgcg gctgcggtgg cggtggcaac gccggaaggc
    53701 ttaacaacgg tagcaaggta ataaacgtgc ccgccgccgc cagcccgtag ttccaggcta
    53761 cgccaccggt cgccagcaat aaaccgccaa tcatgggcga aatcaccgac cccagacgca
    53821 cggtcaacat ggtgatcgcc ccggcctgca ttaagttttc acgccctacc agtgctggtg
    53881 tcgccgccaa tagcgccgta acgccaagcg atgcgaaaaa accatcccat aaaccaagta
    53941 aatagattgc cagcaatgac ggctccggca gcagtgcatt aaggcacagt ccaatgaagc
    54001 caatgccaca ggtgccgcgc gccagcaaaa tcactttttt gcgctcatag cgatccgcca
    54061 gcacaccgcc gaccatcagg ccaacaaaca tcgcgccgcc ggtcagcgtc accgaaagcc
    54121 ccacctgcca ggtggaatgt gtcatcatct ggatctgcac cggcaccgcg acgccgagca
    54181 aacccagaga cacaattgag atgaaacgag cgaggaatac tgcgcgaaac gccgggtgcg
    54241 ttttcaacag gctgaggtta agcagccagg attgtttatt cattacaatg ccttgccatc
    54301 aaatatttta aatacctatt cttaggctgc acatgctaac atatccaaat aagatcgata
    54361 acgataatta atttcattat catggaagtt cgtatgtctg gttctgttgc cgtgacacgc
    54421 gccattgccg tgcccggatt gctgttatta ctgattatcg cgacggcatt aagcctgctc
    54481 attggggcaa aatcactccc cgcttccgta gtgctggagg ccttctccgg cacctgccag
    54541 agcgccgact gcaccatcgt gctcgacgcg cggctgccgc gtacccttgc cggtttactg
    54601 gcaggcggcg cgcttggcct tgccggggcg ttaatgcaaa ccctcacccg aaacccactt
    54661 gccgaccccg gcttgcttgg cgtgaacgcc ggagccagct ttgccattgt gctgggtgcg
    54721 gcgctgtttg gttactcttc cgcgcaggaa caactggcga tggccttcgc cggggcgctg
    54781 gtggcctcat tgattgttgc ctttaccggc agtcagggcg gcgggcagtt aagtccggtg
    54841 cgtttaaccc tggcgggcgt ggcgctggcg gcggtgctgg aaggactgac cagcggcatc
    54901 gccctgctta atcctgacgt ctacgatcag ttgcgtttct ggcaagccgg ttcgctggat
    54961 attcgcaatc tacatacctt aaaagtggtg ctgatcccgg tgctgatcgc cggagcaact
    55021 gcgctattac tgagtcgcgc gctgaacagt ttgagcctcg gcagcgacac cgcgacggcg
    55081 ctgggcagtc gcgtggcgcg cacacagttg attggtctgc tggcgattac cgtgctttgt
    55141 ggtagtgcga cggcaatagt tggcccgatt gcctttattg gcctgatgat gccgcatatg
    55201 gcgcgttggc tggtgggtgc cgatcatcgc tggtcgctgc ccgtcacgct acttgctacc
    55261 cctgccctgc tgctgtttgc cgatatcatc gggcgggtga ttgtgcccgg cgaactgcgc
    55321 gtttctgtgg tcagtgcgtt tattggtgca ccggtgctga tcttcctcgt gcgacgtaaa
    55381 acgcgaggtg gtgcatgatt tacgtctctc gccgattact catcacctgt ttgctgctgg
    55441 tttccgcctg tgtggttgca ggtatctggg gattacgcag cggtgccgtc acgctggaaa
    55501 cctcgcaggt attcgccgcg ctgatgggcg atgcgccgcg cagtatgacg atggtggtca
    55561 ccgaatggcg tttaccacgc gtgctgatgg cgctgttgat tggcgcagca ctgggcgtca
    55621 gtggcgcgat ttttcagtcg ctgatgcgta acccgctcgg cagccctgac gtaatgggct
    55681 ttaacaccgg ggcgtggagc ggcgtgctgg tggcgatggt gctgtttggt caggacctga
    55741 cggctatcgc gctgtcagca atggtgggcg gcattgtcac ttcgctgctg gtctggctgc
    55801 tcgcctggcg caacggcatc gacacctttc ggttgattat tatcggtatc ggcgttcgcg
    55861 ccatgctggt ggcctttaat acctggctgt tgctgaaagc gtctttagaa acggcgctaa
    55921 cagcaggttt gtggaatgcc ggatcgctca acggcctgac gtgggcaaaa acctcgcctt
    55981 ccgcacccat cattatattg atgctcattg ccgccgcctt actggtacgc cggatgcgct
    56041 tgctggaaat gggcgatgat accgcgtgtg cgctgggcgt cagcgtcgaa cgttcgcgtc
    56101 tgttaatgat gctggttgca gtggtgctta ccgctgcggc aacagcgctt gccgggccga
    56161 tttcctttat tgctttagtc gcaccgcaca ttgcccgacg cattagcggc accgctcgct
    56221 gggggctaac ccaggcggcg ctatgcgggg cgctgttact gctggcggcc gatctctgcg
    56281 cccaacaact gtttatgccg tatcaacttc cggttggcgt cgttaccgtc agcctcggcg
    56341 gtatttacct tatcgtcttg ttaattcagg agtctcgcaa aaaatgaccg aatcagtagc
    56401 ccgtttgcgc ggcgaacagt taaccctggg atatggcaaa tataccgttg cggaaaatct
    56461 gactgtagaa atacctgatg gtcacttcac ggcaattatc gggccaaatg gctgcggtaa
    56521 atccacgtta ctgcgtacct taagccgcct gatgacgcct gctcatgggc atgtctggct
    56581 ggatggcgag cacattcaac attacgccag taaagaggtt gcacgccgga ttggtctgtt
    56641 ggcgcaaaat gctaccacgc cgggcgatat caccgtgcag gagctggtgg cgcgtggacg
    56701 ttatccgcat caaccgctgt ttacccgctg gcgcaaagag gatgaagaag cggtaacgaa
    56761 agcaatgcag gccacgggaa taactcatct ggcagatcaa agcgtggata ccctttctgg
    56821 cggacaacgc cagcgagcgt ggatcgcgat ggtgctggcc caggaaacgg caattatgct
    56881 gctcgacgaa cccacgacct ggctggatat cagtcatcag attgatttgc tggagttgtt
    56941 aagcgaactg aaccgcgaaa aaggctatac cctggcggcg gtgctgcacg atcttaatca
    57001 ggcctgtcgt tacgccagcc atttgattgc attgcgggaa gggaaaattg ttgctcaggg
    57061 agcgccgaag gagattgtca ctgctgaact gattgagcgc atttatggtc tgcgctgcat
    57121 gatcattgac gatccagttg ccggaacgcc gcttgtggtg ccgctcggac gaacggcacc
    57181 ttcaaccgca aatagttaaa ctaagtggtc tgccatcatg gcatcctgtt ttctggatgc
    57241 catcgcatag cgcaacagca cgccaccaca agccaccatc ccgccgatca acgcggaaag
    57301 gatcacaata atcgccttac ccggaccgtc ttttttcact ggcaatgacg ggcttaactg
    57361 atatttaaac ggcgtaaaat tcacatcgtt gacatgtgct tttgttaatt gctcgacaag
    57421 atactgccga ttacgtaatt caccgttcag ttccgcaacg tcagtgaccg ctttttctat
    57481 ttccagtttg cgttcaatac cgtctgcacc gagagaaata gaaaaatcgg ggtcatcttt
    57541 aacggcctga ccattactgt aaacgggctt tttaattcct gccgcgttgg caatgtcgag
    57601 tgaataattg aggcgctgaa tgtttgcatc aagttgattt ttcgttttaa tgcgatcctg
    57661 agccagtttt tctttttcaa actgggtttt gatctccagt ttattacgga cgttttctag
    57721 cgactctttc accaccaacg tagagatata atcgatatac ccgctcaaaa cggtctgcgc
    57781 ctcttcactg gttggcgcgg taaaacttag cgtccaggag gtatacagtg acggttcatc
    57841 ttttttctta ctggcattgt catcaaccgc tttcattttt tcgctcaatg cgacaattgc
    57901 gcgatgcaaa tccagttcgt cgattttcgc ctcttttaat tggtccatca cataaggtga
    57961 tgaacgcagg tactcttcca gcaagctaac cgactgaaac ttcttgataa acaggttaaa
    58021 tgcttctgta cgatcaattt tgatatccag atccagcaca cgaagcttag tgaatgattt
    58081 ctccaactct tgccactgaa caggttctgg aggcgtgaca accgccgcgc tggtccattt
    58141 ttgcggcagg atgaaagaga tcagcaagcc tgcgcaggca aacgcaaaaa cgaccgccat
    58201 gaccgttttt ttggcccgcc ataaaactga gattagatta agtaaatcaa tttcattatt
    58261 actgggcgac gccagaggat aatcgggaaa atgagcgtca cttccctgtt taatattcag
    58321 tgatgacatg gggtataacc tgaaaatgag tccaataggg ggaaatctcc acggcgctca
    58381 ctttaccagt tggtgataat tatccgaagc tgaagtttgt aaattccttc cactgaatgc
    58441 aacattcact ctatttaaga gaaatattca tcaaacctat aaatcaaaca aataaaaaac
    58501 ccataattat tttctgtaat tatgggttta taaataatat taatttacct gtttagcgtt
    58561 gcgcgaataa tcggcccaat tttttcaaac gtccctggag agataatatc cacatgcgca
    58621 caatcctgac gatagatatc cagctccgct atccacggcg accaggcgcg ttcgggactc
    58681 ataccttcct gaagtgtgcg ttcagcaaca aacagcgtcg ctttaccgtc aaacggtacg
    58741 ctatgagcag tcgtcagcag gcgcacagca tcagcgtagt tgccttcaat ggtggtaaac
    58801 aactccgttg aagtacttcc ctgctgtgct gccaggaagg cctcgcgttc gcggttaatc
    58861 tccgccagca cttccgggtc cagaccatta gcttcttttt cctgccagtt ttgcgtttct
    58921 ggcggccagg tatccagcaa gccaagaaat gccacctgtt cgccacgggc acgcagtcgc
    58981 gccgcaatac cctgcgccag cgtaccgcca agggaatacc ccagcaggta ataagggcca
    59041 tgcggttgtt gttcaagtaa cgttgccaga tgcgcttcgc agacttcatc caggtttgcc
    59101 gccgtctgca tggggccatt ggggcgcggt gactgaatgc cgataatcga ccattgtgga
    59161 tcgagataac gcgagagcac gctgaactgc caggcaaaac cggacgcagg atggaaacaa
    59221 aacagcgtcg ggccattacc ttcacgcaac ggcagaatgg tttcgaatcc catacgccgg
    59281 gtgctgtctt cttcagcatc aataatcgtt gccagtttgg cgacagttga cgcgaccatc
    59341 acttgccccg gcgtcacctg gcgggcaacc tgccgactta actgcgctgc cagtttcatt
    59401 gccagtagcg aatgaccgcc aagcgcgaag aaatcagcat cggcatcctg cacgtcacac
    59461 cccagcaacg acgagaatgc cgcggcgata atcgtttcac tgcccgcttt cggcgcacgc
    59521 cctggcgctt gtgccttcag ttcaggcaac ggtaaggctt tgcgatccag cttgccgttg
    59581 gcgctaagtg gtaactgtgg aagttgcagc agaaccaccg gtaccatatg tggtggcaat
    59641 gtttcacgaa gctgcgcctg caatgcgctg gtatccaacg gcaggcccga ttgcgacacc
    59701 agatagccca ccaattgacg cgcatcacca ccggtggcag ccgcctggtt aatcacacag
    59761 gcgtgggtaa cggcttgttc gacatccggc agcgcctgca tcacgcgatc gatttcgccc
    59821 agttcgatac gctgcccgcg aatttttagc tgatcatcac tgcgcccgag gtactccacc
    59881 gcgccgttat ccagccagcg ggcaacgtct ccggtacggt acatccgttc acctggggca
    59941 aaaggatcgg caataaagcg gctggcggtc agatcggggc gtccgagata gccctgcgcc
    60001 agttgaatgc cagtgagata gagatcaccc gccacacccg gcggcaccgg atgcatcatc
    60061 gcatcaagaa tacgcagacc cgtattccat accggataac caatcggcac actgctgccg
    60121 cgcacctgtg ccagttcctc gccaaaagcc ggataccagc tgacatctac cgccgcttcc
    60181 gtcgggccat atagattatg caacggcgcg ccagttaact gttgccattc gcggcataaa
    60241 tcagccggta aggcctcacc actacagaaa acctgtttca acgtcgcgca actctggcga
    60301 gcggtttgcg gcgtcagcga ggcaacaaat gccgccagca tcgacggcac aaagtgcgtg
    60361 gtcgttacgc catattcggc aaagaattgc tgcatagcga gcgggtcgcg gtgcgcttcc
    60421 ggttcagcca tcaccagttt tgcccctgcg ataaacggcc agaaaaactc ccacaccgag
    60481 acatcaaaac tgcacggcgt tttttgggca acgacatctt cgcctgtaag cggataatga
    60541 ttttgcatcc aaagcaggcg gttgacgata gccgtctgcc cgaccattac ccctttcggc
    60601 ctgccggtgg agccagaggt aaagatgata taagccgtgt gatgcggttg tgaaagttgc
    60661 agcggcgcac tgccctgcgg tgtaagcggg gcgttatagc aaaggcttgt taaattggga
    60721 acatcgctaa agcgcggcag ttgatcgtcg gtggtaatta acagcgacgg acgcgcatct
    60781 tccagcatca ttttcaggcg atcgtccgga tagccggtat ccagcggtag ccaggccgct
    60841 ccagcttcaa ctatcgcatg gagtgccagg gtcaaaaaga ccgagcgcgg tagtgccacc
    60901 gccacgctgt cccctggttt aacgccgcgc tcacgcagca gattcgccag cgccaccacc
    60961 tgctcgcgca tttcccgata gctgaacagg taacgcgcat ctgccagcgc cggagcatcc
    61021 ggtgtttttg ccgcttgttc tgccaccagc gcgctaagcg tggtttctgg aatctcaacc
    61081 tgagtggcgt tgagctgcgc cagctgcgca tactcacctg gcagcataat atcgacatcg
    61141 ccgcacaaca gcgccggatc cgcggcgaac tgggcaatca gcattttcag gcgttcagca
    61201 tgctggatta acgttggctc atcgtaacgc tgtttattgg cgaggatctc aatactcaaa
    61261 tcaccgtgta catccgggaa cagggccagt tcaaggtcat taaccggacc ggttgccagg
    61321 gtatgggttt gcgcctgaac atcaggaata tccagttggt aatcaaatac cttgatattg
    61381 agtaccggac caaacagcgg ttcatcacct gccgctcgcc cgctgtcacg gacaatttgt
    61441 tcggcatcgt aacgttgatg acgacgcatt tttttcagtt gtgctgccag tcgggttgcc
    61501 agttccggca gcgtttcttg cgccgcaatg tgaataccca acggcaaaac gttgagcacg
    61561 ggtccggtag ccgtcagcgc cgccgagccc agtcgacgca taaagataaa tccggcggcg
    61621 tagtccatac gattgcacaa tcgccccagc cacaaggctg ccagcgcaag ggctaaatcg
    61681 gtacgctgca cacctgaaag ttgcgtagcc agctggcgga attccccgtc ggtaaattcc
    61741 agtttcaggc gcagaatatc tgccgaggcg ctgcgccccg gtaaaggtgc cggagaaagt
    61801 gacgcgggcg gcggcagttg acgacgctgt tctgcccaga atgccgcatc acgctgccag
    61861 gcttcgcttt cgcggtattg ctggtactct tccactacat cagcgaaagg cgtaaatggc
    61921 gaagcaggcg ttggttcgcc acgcagccat gtgcagtaaa tattggcgat ctggcgggta
    61981 atggccggga aactaaagcc atcgaccagc aaatggtgat aacgctgata ccagtaccag
    62041 cggttatccg ccacctgtat cagctgatga aagaccagtg gtttaccgct atcgacgcgc
    62101 agatcttgtt gcaaatccgc ctgcattaat gcctgcgcag taccgtgcgg atcaatgttg
    62161 gttcgtaggt cgataatttc tggcagttcg aacgtcagcg catcatcgac ccactgccag
    62221 acttcgccgt tatcttccgt aaaacgcatc cgcagcgtat ctgcttgcgc tagtccggca
    62281 accaccgcgc gggccagtaa tggcgaatca acctctccgg ttaactcaac gtaatgcgcc
    62341 acgctccagg cggagggtaa ttctgacagt ttttctgcca tccagatgcc gggctgtgcg
    62401 gcgaccaaag gtaaatgctg gctcattgtg cctcctgcaa ctgggtaaaa ttcgtcggtg
    62461 tcagagtacg ccagtgggct tccagccact gctggcagga cgcctgtgac tgcggctgac
    62521 acacaatgtc ccagcctgcc ggtaagacgc attgttgcgg ccacagactg aattgcccct
    62581 gcgcattgcg caatatgtaa aacgctccct gcggatcatc gaagggatta ctgaatgcca
    62641 tattcaactc ctgtcatgga aaagtggttg ccagaggtcg attagcccct gcatcaagcc
    62701 accgcgccaa caaagcgcat catgtccgcc gtcaacctga cgccagaaaa tggattcttt
    62761 tatggggtgt aattgcgcat acagcgcctg attggcccgc atgatcatcg gctcgcgaat
    62821 acccgcttcc agcacaatgc gcagaccttc ggcgctaact tcaccagctt ttagcttttc
    62881 aagtaacacg ccctcttgct gcccgccccg atgcggccac cagtacgatc ctgactggct
    62941 taatacacag ccaaagcgtt caggccagtg cagtccggca tacagcgcgg aaagcccacc
    63001 aaaactctgc ccggcaacca cggtgcgatc ggcacgatcg ctaaaagggg caatagcttt
    63061 caccaggggt aataactctt gctgtactgc gagccagaaa tccgcattac acggcagttc
    63121 gtgggcgcgg tgcgtggtgt cgatagcgtc gatcaacaca tacacggcgg gaggaagttg
    63181 ctgacgatgg gtcagcgaag tcagcactgg ccagacgggc atactttgcg cccaaaattc
    63241 gccatcgagc aaaactgcca gcgggcgttc ttctgctgtt gcatcgccgg tggtaaaaat
    63301 ccatacacgc cgtgactttt tcaaccgttc acttttccag ataatttctt tggcaggtat
    63361 ttctggcgct tgcggacaat cccatcccgg ttgcagaggc gcttgcggca tttcgagtgc
    63421 agaaacagcg tgccctcgcc cgcctttcca gctttgtagg ttcagcggat cggctatcgc
    63481 ctggggtaat agttttcgcc agccttcgcg caattcgagg cgatcggggg atggtacaga
    63541 aaaaatgtca tcgcgttcgg tgggaataaa gcagtagctg ccgcgccagt tggcattgag
    63601 ttgtgtcgtc cactgccaga cgttagtgcc tgcaattcgc tgcatcgact ggggctggct
    63661 gttctgatgg tgatcggtca caccagtgat gtagacccat acgcgcttta tcgtcgagta
    63721 ttcttcagaa ccttggggat cacgccacca gaaagtgacc tcaaacattt cgtcattcag
    63781 acgctgccat tccgggccat gtttcgactg ccaccagctc tcacttccta cttttaacgc
    63841 cgtcacacca taaccccatg tttactgtgc aatttttcat tgattgcaga aatatattga
    63901 taatattatt gataactatt tgcatttgca atagcgtaat ggcgcgccgt gggaagcgcg
    63961 gacattaatt aaccaactgc actgcgtgtc tttcaggatc aaaggttttc gcggtagcgg
    64021 gatgcgtcgt gttgatgacg accatgcccg acagttgcaa ttcgtggcaa aaatgcagga
    64081 ataaaacaat gaacaagaag attcattccc tggccttgtt ggtcaatctg gggatttatg
    64141 gggtagcgca ggcacaagag ccgaccgata ctcctgtttc acatgacgat actattgtcg
    64201 ttaccgccgc cgagcagaac ttacaggcgc ctggcgtttc gaccatcacc gcagatgaaa
    64261 tccgcaaaaa cccggttgcc cgcgatgtgt cggagatcat ccgtaccatg ccaggcgtta
    64321 acctgaccgg taactccacc agtggtcagc gtgggaataa ccgacagatt gatattcgcg
    64381 gtatgggtcc ggaaaacacg ctgattttga ttgacggcaa gccggtaagc agccgtaact
    64441 cggtgcgtca gggctggcgt ggcgagcgcg atacccgtgg tgatacttcc tgggtgccac
    64501 ctgaaatgat tgaacgtatt gaagttctgc gtggtccggc agctgcgcgt tatggcaacg
    64561 gcgcggcggg cggcgtggtt aacatcatta ccaaaaaagg cagcggcgag tggcacggct
    64621 cctgggacgc atatttcaat gcgccagaac ataaagagga aggtgccacc aaacgcacta
    64681 actttagcct gaccggtccg ctgggcgacg aattcagctt ccgtttgtat ggcaacctcg
    64741 acaaaaccca ggctgacgcg tgggatatca accagggcca tcagtccgcg cgtgccggaa
    64801 cgtatgccac gacgttacca gccgggcgcg aaggggtaat caacaaagat attaatggcg
    64861 tggtgcgctg ggatttcgcg ccattgcaat cgctggaact ggaagcaggt tacagccgcc
    64921 agggtaacct gtatgcgggc gacacccaga ataccaactc cgattcctat acccgctcga
    64981 aatatggcga tgaaaccaac cgtctgtatc gccagaacta cgcgctgacc tggaacggtg
    65041 gctgggataa cggcgtgacc accagcaact gggtgcagta cgaacacacc cgtaactcgc
    65101 gtattccgga aggtctggcg ggcggtaccg aagggaaatt taacgaaaaa gcgacacagg
    65161 atttcgtcga tatcgatctt gatgacgtga tgctgcacag cgaagttaac ctgccgattg
    65221 atttcctcgt taaccagacg ctgacgctgg gtacggagtg gaatcagcaa cggatgaagg
    65281 acttaagttc caacacccag gcactgaccg gaacgaatac cggtggcgct attgatggcg
    65341 tgagtaccac cgaccgtagc ccgtattcaa aagcagaaat tttctcgctg tttgccgaaa
    65401 acaacatgga gctgactgac agcaccatcg taacgccggg gctgcgtttc gatcatcaca
    65461 gtattgtcgg caataactgg agcccggcgc tgaacatatc gcaaggttta ggcgatgact
    65521 tcacgctgaa aatgggcatc gcccgtgctt ataaagcgcc gagcctgtac cagactaacc
    65581 cgaactacat tctctacagt aaaggtcagg gttgctatgc cagcgcgggc ggctgctatc
    65641 tgcaaggtaa cgatgacctg aaagcagaaa ccagcatcaa caaagagatt ggtctggagt
    65701 tcaaacgcga cgggtggctg gcgggcgtca cctggttccg taacgattat cgcaataaga
    65761 ttgaagcggg ctatgtggct gtagggcaaa acgcagtcgg caccgatctc tatcagtggg
    65821 ataacgtgcc gaaagcggtg gttgaaggtc tggaaggatc gttaaacgta ccggttagcg
    65881 aaacggtgat gtggaccaat aacatcactt atatgctgaa gagtgaaaac aaaaccacgg
    65941 gcgaccgttt gtcgatcatc ccggagtata cgttgaactc aacgctgagc tggcaggcac
    66001 gggaagattt gtcgatgcaa acgaccttca cctggtacgg caagcagcag ccgaagaagt
    66061 acaactataa aggtcagcca gcggttggac cggaaaccaa agaaattagt ccttacagca
    66121 ttgttggcct gagcgcgacc tgggatgtga cgaagaatgt cagtctgacc ggcggcgtgg
    66181 acaatctgtt cgacaaacgt ttgtggcgtg cgggtaatgc ccagaccacg ggcgatttgg
    66241 caggggccaa ctatatcgcc ggtgccgggg cgtataccta taacgagccg ggacgtacgt
    66301 ggtatatgag cgtaaacacc cacttctgat gctaacgtca gattgttgac aaagtgcgcg
    66361 tcgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaaatct tgccaattca
    66421 atatattgca ggaccatgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgt
    66481 catcagtctc gaatatggtc gatatgaaaa ctacgcatac ctccctcccc tttgccggac
    66541 atacgctgca ttttgttgag ttcgatccgg cgaatttttg tgagcaggat ttactctggc
    66601 tgccgcacta cgcacaactg caacacgctg gacgtaaacg taaaacagag catttagccg
    66661 gacggatcgc tgctgtttat gctttgcggg aatatggcta taaatgtgtg cccgcaatcg
    66721 gcgagctacg ccaacctgtc tggcctgcgg aggtatacgg cagtattagc cactgtggga
    66781 ctacggcatt agccgtggta tctcgtcaac cgattggcat tgatatagaa gaaatttttt
    66841 ctgtacaaac cgcaagagaa ttgacagaca acattattac accagcggaa cacgagcgac
    66901 tcgcagactg cggtttagcc ttttctctgg cgctgacact ggcattttcc gccaaagaga
    66961 gcgcatttaa ggcaagtgag atccaaactg atgcaggttt tctggactat cagataatta
    67021 gctggaataa acagcaggtc atcattcatc gtgagaatga gatgtttgct gtgcactggc
    67081 agataaaaga aaagatagtc ataacgctgt gccaacacga ttaattgaca acatctggta
    67141 cgattcgccc gcagccatca ctgaccacgg gcgaaagtgt aaagcaggtg ccttaccatc
    67201 ctgacctgac aaccggatat gcggggat
//
LOCUS       DBBGLQ010000020        66595 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.020, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000020 DBBGLQ010000000
VERSION     DBBGLQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.020"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            256..1299
                     /gene="nadA"
                     /locus_tag="ACIYSB_001643"
     CDS             256..1299
                     /gene="nadA"
                     /locus_tag="ACIYSB_001643"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGC5428719.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1337..2056
                     /gene="pnuC"
                     /locus_tag="ACIYSB_001644"
     CDS             1337..2056
                     /gene="pnuC"
                     /locus_tag="ACIYSB_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGC5428720.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2053..2994)
                     /gene="zitB"
                     /locus_tag="ACIYSB_001645"
     CDS             complement(2053..2994)
                     /gene="zitB"
                     /locus_tag="ACIYSB_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGC5428721.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3108..3488)
                     /gene="ybgS"
                     /locus_tag="ACIYSB_001646"
     CDS             complement(3108..3488)
                     /gene="ybgS"
                     /locus_tag="ACIYSB_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGC5428722.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3804..4856
                     /gene="aroG"
                     /locus_tag="ACIYSB_001647"
     CDS             3804..4856
                     /gene="aroG"
                     /locus_tag="ACIYSB_001647"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGC5428723.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(5014..5766)
                     /gene="gpmA"
                     /locus_tag="ACIYSB_001648"
     CDS             complement(5014..5766)
                     /gene="gpmA"
                     /locus_tag="ACIYSB_001648"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGC5428724.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5968..7008)
                     /gene="galM"
                     /locus_tag="ACIYSB_001649"
     CDS             complement(5968..7008)
                     /gene="galM"
                     /locus_tag="ACIYSB_001649"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGC5428725.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(7002..8150)
                     /gene="galK"
                     /locus_tag="ACIYSB_001650"
     CDS             complement(7002..8150)
                     /gene="galK"
                     /locus_tag="ACIYSB_001650"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGC5428726.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8154..9200)
                     /gene="galT"
                     /locus_tag="ACIYSB_001651"
     CDS             complement(8154..9200)
                     /gene="galT"
                     /locus_tag="ACIYSB_001651"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGC5428727.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9210..10226)
                     /gene="galE"
                     /locus_tag="ACIYSB_001652"
     CDS             complement(9210..10226)
                     /gene="galE"
                     /locus_tag="ACIYSB_001652"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGC5428728.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10487..11959)
                     /gene="modF"
                     /locus_tag="ACIYSB_001653"
     CDS             complement(10487..11959)
                     /gene="modF"
                     /locus_tag="ACIYSB_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGC5428729.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(12027..12815)
                     /gene="modE"
                     /locus_tag="ACIYSB_001654"
     CDS             complement(12027..12815)
                     /gene="modE"
                     /locus_tag="ACIYSB_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGC5428730.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12944..13093
                     /gene="acrZ"
                     /locus_tag="ACIYSB_001655"
     CDS             12944..13093
                     /gene="acrZ"
                     /locus_tag="ACIYSB_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGC5428731.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13260..14033
                     /gene="modA"
                     /locus_tag="ACIYSB_001656"
     CDS             13260..14033
                     /gene="modA"
                     /locus_tag="ACIYSB_001656"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGC5428732.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQFKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASVQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTIK"
     gene            14033..14722
                     /gene="modB"
                     /locus_tag="ACIYSB_001657"
     CDS             14033..14722
                     /gene="modB"
                     /locus_tag="ACIYSB_001657"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGC5428733.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14725..15783
                     /gene="modC"
                     /locus_tag="ACIYSB_001658"
     CDS             14725..15783
                     /gene="modC"
                     /locus_tag="ACIYSB_001658"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGC5428734.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15784..16602)
                     /gene="ybhA"
                     /locus_tag="ACIYSB_001659"
     CDS             complement(15784..16602)
                     /gene="ybhA"
                     /locus_tag="ACIYSB_001659"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGC5428735.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVIKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16757..17752
                     /gene="pgl"
                     /locus_tag="ACIYSB_001660"
     CDS             16757..17752
                     /gene="pgl"
                     /locus_tag="ACIYSB_001660"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGC5428736.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17793..18746)
                     /gene="ybhD"
                     /locus_tag="ACIYSB_001661"
     CDS             complement(17793..18746)
                     /gene="ybhD"
                     /locus_tag="ACIYSB_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5428737.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            18930..19982
                     /gene="ybhH"
                     /locus_tag="ACIYSB_001662"
     CDS             18930..19982
                     /gene="ybhH"
                     /locus_tag="ACIYSB_001662"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGC5428738.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20058..21491
                     /gene="ybhI"
                     /locus_tag="ACIYSB_001663"
     CDS             20058..21491
                     /gene="ybhI"
                     /locus_tag="ACIYSB_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGC5428739.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            21674..23935
                     /gene="ybhJ"
                     /locus_tag="ACIYSB_001664"
     CDS             21674..23935
                     /gene="ybhJ"
                     /locus_tag="ACIYSB_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGC5428740.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVSRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(24169..25452)
                     /gene="ybhC"
                     /locus_tag="ACIYSB_001665"
     CDS             complement(24169..25452)
                     /gene="ybhC"
                     /locus_tag="ACIYSB_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGC5428741.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(25604..26080)
                     /gene="ybhB"
                     /locus_tag="ACIYSB_001666"
     CDS             complement(25604..26080)
                     /gene="ybhB"
                     /locus_tag="ACIYSB_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGC5428742.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(26139..27428)
                     /gene="bioA"
                     /locus_tag="ACIYSB_001667"
     CDS             complement(26139..27428)
                     /gene="bioA"
                     /locus_tag="ACIYSB_001667"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGC5428743.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTAQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            27515..28555
                     /gene="bioB"
                     /locus_tag="ACIYSB_001668"
     CDS             27515..28555
                     /gene="bioB"
                     /locus_tag="ACIYSB_001668"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGC5428744.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            28552..29706
                     /gene="bioF"
                     /locus_tag="ACIYSB_001669"
     CDS             28552..29706
                     /gene="bioF"
                     /locus_tag="ACIYSB_001669"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGC5428745.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            29693..30448
                     /gene="bioC"
                     /locus_tag="ACIYSB_001670"
     CDS             29693..30448
                     /gene="bioC"
                     /locus_tag="ACIYSB_001670"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGC5428746.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            30441..31118
                     /gene="bioD"
                     /locus_tag="ACIYSB_001671"
     CDS             30441..31118
                     /gene="bioD"
                     /locus_tag="ACIYSB_001671"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGC5428747.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            31697..33718
                     /gene="uvrB"
                     /locus_tag="ACIYSB_001672"
     CDS             31697..33718
                     /gene="uvrB"
                     /locus_tag="ACIYSB_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGC5428748.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(33910..34818)
                     /gene="yvcK"
                     /locus_tag="ACIYSB_001673"
     CDS             complement(33910..34818)
                     /gene="yvcK"
                     /locus_tag="ACIYSB_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGC5428749.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      35083..35227
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            35215..36204
                     /gene="moaA"
                     /locus_tag="ACIYSB_001674"
     CDS             35215..36204
                     /gene="moaA"
                     /locus_tag="ACIYSB_001674"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGC5428750.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVASWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            36226..36738
                     /gene="moaB"
                     /locus_tag="ACIYSB_001675"
     CDS             36226..36738
                     /gene="moaB"
                     /locus_tag="ACIYSB_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGC5428751.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            36741..37226
                     /gene="moaC"
                     /locus_tag="ACIYSB_001676"
     CDS             36741..37226
                     /gene="moaC"
                     /locus_tag="ACIYSB_001676"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGC5428752.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            37219..37464
                     /gene="moaD"
                     /locus_tag="ACIYSB_001677"
     CDS             37219..37464
                     /gene="moaD"
                     /locus_tag="ACIYSB_001677"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGC5428753.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDSWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            37466..37918
                     /gene="moaE"
                     /locus_tag="ACIYSB_001678"
     CDS             37466..37918
                     /gene="moaE"
                     /locus_tag="ACIYSB_001678"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGC5428754.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVNALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            38055..38759
                     /gene="ybhL"
                     /locus_tag="ACIYSB_001679"
     CDS             38055..38759
                     /gene="ybhL"
                     /locus_tag="ACIYSB_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGC5428755.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            38964..39677
                     /gene="ybhM"
                     /locus_tag="ACIYSB_001680"
     CDS             38964..39677
                     /gene="ybhM"
                     /locus_tag="ACIYSB_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGC5428756.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(39713..40669)
                     /gene="ybhN"
                     /locus_tag="ACIYSB_001681"
     CDS             complement(39713..40669)
                     /gene="ybhN"
                     /locus_tag="ACIYSB_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGC5428757.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(40669..41910)
                     /gene="clsB"
                     /locus_tag="ACIYSB_001682"
     CDS             complement(40669..41910)
                     /gene="clsB"
                     /locus_tag="ACIYSB_001682"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGC5428758.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(41907..42668)
                     /gene="ybhP"
                     /locus_tag="ACIYSB_001683"
     CDS             complement(41907..42668)
                     /gene="ybhP"
                     /locus_tag="ACIYSB_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGC5428759.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            42801..43211
                     /gene="ybhQ"
                     /locus_tag="ACIYSB_001684"
     CDS             42801..43211
                     /gene="ybhQ"
                     /locus_tag="ACIYSB_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGC5428760.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(43173..44279)
                     /gene="ybhR"
                     /locus_tag="ACIYSB_001685"
     CDS             complement(43173..44279)
                     /gene="ybhR"
                     /locus_tag="ACIYSB_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5428761.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(44290..45423)
                     /gene="ybhS"
                     /locus_tag="ACIYSB_001686"
     CDS             complement(44290..45423)
                     /gene="ybhS"
                     /locus_tag="ACIYSB_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5428762.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(45416..47152)
                     /gene="ybhF"
                     /locus_tag="ACIYSB_001687"
     CDS             complement(45416..47152)
                     /gene="ybhF"
                     /locus_tag="ACIYSB_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGC5428763.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(47145..48140)
                     /gene="hlyD"
                     /locus_tag="ACIYSB_001688"
     CDS             complement(47145..48140)
                     /gene="hlyD"
                     /locus_tag="ACIYSB_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGC5428764.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(48143..48814)
                     /gene="cecR"
                     /locus_tag="ACIYSB_001689"
     CDS             complement(48143..48814)
                     /gene="cecR"
                     /locus_tag="ACIYSB_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGC5428765.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            49043..50407
                     /gene="rhlE"
                     /locus_tag="ACIYSB_001690"
     CDS             49043..50407
                     /gene="rhlE"
                     /locus_tag="ACIYSB_001690"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGC5428766.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(50639..51121)
                     /gene="ybiA"
                     /locus_tag="ACIYSB_001691"
     CDS             complement(50639..51121)
                     /gene="ybiA"
                     /locus_tag="ACIYSB_001691"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HGC5428767.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGHGKGKNRLGYLLMELREQLAIEK
                     "
     gene            51241..53391
                     /gene="dinG"
                     /locus_tag="ACIYSB_001692"
     CDS             51241..53391
                     /gene="dinG"
                     /locus_tag="ACIYSB_001692"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGC5428768.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            53419..54381
                     /gene="ybiB"
                     /locus_tag="ACIYSB_001693"
     CDS             53419..54381
                     /gene="ybiB"
                     /locus_tag="ACIYSB_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGC5428769.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            54522..55607
                     /gene="hcxB"
                     /locus_tag="ACIYSB_001694"
     CDS             54522..55607
                     /gene="hcxB"
                     /locus_tag="ACIYSB_001694"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGC5428770.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            55674..55733
                     /gene="ybiE"
                     /locus_tag="ACIYSB_001695"
     CDS             55674..55733
                     /gene="ybiE"
                     /locus_tag="ACIYSB_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGC5428771.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(55836..56096)
                     /gene="ybiJ"
                     /locus_tag="ACIYSB_001696"
     CDS             complement(55836..56096)
                     /gene="ybiJ"
                     /locus_tag="ACIYSB_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGC5428772.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(56361..56627)
                     /gene="ybiI"
                     /locus_tag="ACIYSB_001697"
     CDS             complement(56361..56627)
                     /gene="ybiI"
                     /locus_tag="ACIYSB_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGC5428773.1"
                     /translation="MASSWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(56701..57378)
                     /gene="ybiX"
                     /locus_tag="ACIYSB_001698"
     CDS             complement(56701..57378)
                     /gene="ybiX"
                     /locus_tag="ACIYSB_001698"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGC5428774.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(57420..59702)
                     /gene="fiu"
                     /locus_tag="ACIYSB_001699"
     CDS             complement(57420..59702)
                     /gene="fiu"
                     /locus_tag="ACIYSB_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGC5428775.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(59967..60227)
                     /gene="mcbA"
                     /locus_tag="ACIYSB_001700"
     CDS             complement(59967..60227)
                     /gene="mcbA"
                     /locus_tag="ACIYSB_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGC5428776.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEYKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            60503..61429
                     /gene="rlmF"
                     /locus_tag="ACIYSB_001701"
     CDS             60503..61429
                     /gene="rlmF"
                     /locus_tag="ACIYSB_001701"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGC5428777.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(61426..63651)
                     /gene="ybiO"
                     /locus_tag="ACIYSB_001702"
     CDS             complement(61426..63651)
                     /gene="ybiO"
                     /locus_tag="ACIYSB_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGC5428778.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(63912..64634)
                     /gene="glnQ"
                     /locus_tag="ACIYSB_001703"
     CDS             complement(63912..64634)
                     /gene="glnQ"
                     /locus_tag="ACIYSB_001703"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGC5428779.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(64631..65290)
                     /gene="glnP"
                     /locus_tag="ACIYSB_001704"
     CDS             complement(64631..65290)
                     /gene="glnP"
                     /locus_tag="ACIYSB_001704"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGC5428780.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(65598..66089)
                     /locus_tag="ACIYSB_001705"
     CDS             complement(65598..66089)
                     /locus_tag="ACIYSB_001705"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGC5428781.1"
                     /translation="MEEDNFPEISEANILSTAEQLHRNRYEMAERGVINLFRGLSRTH
                     NSRSPCKFGSKIILNNLVKWDWWGFGLNHGPQAGQLADLERILYQFSGKPIPDYRDNI
                     IIRLDEHIRSVQGKACYEDEMFSIRYFRKGSAHITFKHPELVEKLNAVLAKHYSVKFH
                     SSV"
     gene            complement(66102..66446)
                     /locus_tag="ACIYSB_001706"
     CDS             complement(66102..66446)
                     /locus_tag="ACIYSB_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGC5428782.1"
                     /translation="MSDITISRPEALNGHTDLICSTDIRHVVTVRNEALQQTERLIRQ
                     LAEISAMTESIGGKTVRDWAVKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDVQTRD"
BASE COUNT        15819 a        16979 c        17728 g        16069 t
ORIGIN      
        1 gcgctttttt gctattcagg catcctcaat ttcactttgt aaacctgatg acatcgtcag
       61 agcttactgt gcaagcaact ctatgtcggt ggaattaggc gtaaaatgac gcatcctgca
      121 cattaggcgt aattcgagtg acttttcccc accattcgac tatcttgttt agcatataaa
      181 acaaattaca ccgataacag cgaatattac gctaatgtcg gttttaacgt taagcctgta
      241 aaacgagatg gtaagatgag cgtaatgttt gatccagaca cggcgattta tcctttcccc
      301 ccgaagccga cgccgttaag cattgatgaa aaagcgtatt accgcgagaa gataaaacgt
      361 ctgctaaaag aacgtaatgc ggtgatggtt gcccactact ataccgatcc cgaaattcaa
      421 caactggcag aagaaaccgg tggctgtatt tctgattctc tggaaatggc gcgcttcggt
      481 gcaaagcatc ccgcttctac tttgttagtc gctggggtga gatttatggg agaaaccgcc
      541 aaaattctca gtccggaaaa aacaattctg atgccgacac ttcaggctga atgttcactg
      601 gatctcggct gccctgttga agaatttaac gcattttgcg atgcccatcc cgatcgtact
      661 gtcgtcgtct acgccaacac ttctgctgcg gtaaaagcgc gcgcagattg ggtggtaact
      721 tcaagcattg ccgtcgaact tattgatcat cttgatagtt tgggtgaaaa aatcatctgg
      781 gcacccgaca aacatctggg gcgttacgtg caaaaacaga cgggtggaga cattctatgc
      841 tggcagggtg cctgtattgt gcatgatgaa tttaagactc aggcgttaac ccgcttgcaa
      901 gaagaatacc cggatgctgc catactggtg catccagaat caccacaagc tattgtcgat
      961 atggcggatg cggtcggttc caccagtcaa ctgatcgctg ctgcgaaaac attgccacat
     1021 cagaggctta ttgtggcaac cgatcggggt attttctaca aaatgcagca ggcggtgcca
     1081 gataaagagt tactggaagc accaaccgca ggtgagggtg caacctgccg cagctgcgcg
     1141 cattgtccgt ggatggccat gaatggcctt caggccatcg cagaggcatt agaacaggaa
     1201 ggaagcaatc acgaggttca tgttgatgaa aggctgcgag agagggcgct ggtgccgctc
     1261 aatcgtatgc tggattttgc ggctacacta cgtggataac gaataataag gcgtaacgtt
     1321 acgctttggg ggaaagatgg atttttttag tgtgcagaat atcctggtac atataccaat
     1381 aggggcaggc ggttatgatc tctcatggat cgaagcggta ggcacgatcg ccgggttgct
     1441 gtgtattggc cttgccagtc tggagaagat cagcaactac ttctttggcc tgatcaacgt
     1501 caccttgttt ggcattattt tctttcagat tcagctgtat gccagcctgc tattacaggt
     1561 gtttttcttt gccgcgaata tttacggttg gtatgcgtgg tcgcgacaaa ccagtcagaa
     1621 cgaggcggag ttgaaaattc gctggttgcc attgccgaag gcactcagct ggttggcggt
     1681 ttgcgttgtt tcgattggtc tgatgacggt atttatcaat ccggtgtttg catttttgac
     1741 ccgcgtggca gtcatgatca tgcaagcatt aggattacag gttgtgatgc ctgaactgca
     1801 accggacgct ttcccgttct gggattcatg catgatggtg ttatctatcg tggcaatgat
     1861 tctgatgacg cgtaagtatg tggaaaactg gctgttgtgg gtgattatta acgtgattag
     1921 cgtcgttatt tttgcacttc agggcgttta cgccatgtct ctggagtaca tcatcctgac
     1981 ctttattgcg ctcaacggca gccggatgtg gatcaacagc gcacgtgaaa gaggctcacg
     2041 cgcgctgtcc cattaatggt gatgatgtga atggccagac accccttcat tcaggtggca
     2101 gtctggccca tgacaaggtt gatattccat ctgaatggtg gcgtgctcaa tctgatagtg
     2161 atccatcagg tagtgttgga tctgatccaa caaggcatcg tgatcgtgtg gcgggatcac
     2221 ctgcacatgc aacgtcatca ccggcttctc gcctaccatc catacatgta catggtgcac
     2281 attgcggact tccgggattt cccggcacat acgacgcttc agttcagcga tatccagcga
     2341 taccggtgca ccttcaagta attcattcac gctatctttc aacagtcgcc acgcgctacg
     2401 cagaaccaga agcgacacca gtatcgagag aatggggtca gcaggtgtcc agccagtcca
     2461 gataataatc aacgcggcaa taatagcccc aaccgaaccc agcaggtccc cgagcacatg
     2521 cagtgccgct gctcgtacgt tgaggttttt ctcttcgctg ccgtgatgaa gtaaccagaa
     2581 agaaagtata tttgccagca acccagccac ggcaattgcc atcatcatgc cgccctcgac
     2641 cggacgcggc gtgcggaagc gttctatcgc ctcccagaca atcaaaatgg taattaccac
     2701 gagggctatg gcattcacaa acgccgcgag cgtggtcagt cttagccaac cgaaagtgtg
     2761 gcgaatggtg ggaggacgac gtgaaaattg cacggcgagt agggcgaaaa gcagggcggc
     2821 agtatcggtc aacatatgac ccgcatcggc cagcaatgcc agagaaccag aaagaaaacc
     2881 accaacgact tctaccagca taaacccggc agtcacgccg aaagcataca acaagcgacg
     2941 agcattatta tcttctggca ggtgtgagga cgtgtgtgag tgtgagtgcg ccatgacgag
     3001 tcatccctta attattctca cttaacttta tgacatcata ctgcttttag aagtgaaaaa
     3061 ttaaaaggga gagactccgc tctcccatta ttggctattt tgcagggtta ctgcgtggta
     3121 ccgtcggttt tggtatcgac atcattattg atgccatcac cggtttgtac ctttttattg
     3181 atatccggac agcgaccatc tttgcacatg gtgttcttgt gctcttcatc tttggtcatt
     3241 ccgtcattgt tcattgaaga accatccgaa tgcagcattg tgccgccaga accggtattt
     3301 accccgttat tgtcgacgtt atttggcgcg acattttcac gggcgtcagg ggctacctgg
     3361 cccgcatcag ctgcggcgtt tgcctggccg ttattagttt gcgctccgct atcggcagcc
     3421 agtgcggcac cgctggcaag gcttagagtg gcagtcagaa ataatgtggc cagttttgtc
     3481 attttcatag gatgctcctg ttatggtcgt tatgtcggat aacctcttcc aacagtgcat
     3541 ttgcaggtga atataaggca ttggtttaag atttcagcca ggttatgaaa cgcagcagag
     3601 aatcttgaaa taattaacaa acaaaggagt tacagttaga aattgtagga gagatctcgt
     3661 ttttcgcgac aatctggcgt ttttcttgct aattccagga ttaatccgtt catagtgtaa
     3721 aaccccgttt acacattctg acggaagata tagattggaa gtattgcatt cactaagata
     3781 agtatggcaa cactggaaca gacatgaatt atcagaacga cgatttacgc atcaaagaaa
     3841 tcaaagagtt acttcctcct gtcgcattgc tggaaaaatt ccccgctact gaaaatgccg
     3901 cgaatacggt tgcccatgcc cgaaaagcga tccataagat cctgaaaggt aatgatgatc
     3961 gcctgttggt tgtgattggc ccatgctcaa ttcatgatcc tgtcgcggca aaagagtatg
     4021 ccactcgctt gctggcgctg cgtgaagagc tgaaagatga gctggaaatc gtaatgcgcg
     4081 tctattttga aaagccgcgt accacggtgg gctggaaagg gctgattaac gatccgcata
     4141 tggataatag cttccagatc aacgacggtc tgcgtatagc ccgtaaattg ctgcttgata
     4201 ttaacgacag cggtctgcca gcggcaggtg agtttctcga tatgatcacc ccacaatatc
     4261 tcgctgacct gatgagctgg ggcgcaattg gcgcacgtac caccgaatcg caggtgcacc
     4321 gcgaactggc atcagggctt tcttgtccgg tcggcttcaa aaatggcacc gacggtacga
     4381 ttaaagtggc tatcgatgcc attaatgccg ccggtgcgcc gcactgcttc ctgtccgtaa
     4441 cgaaatgggg gcattcggcg attgtgaata ccagcggtaa cggcgattgc catatcattc
     4501 tgcgcggcgg taaagagcct aactacagcg cgaagcacgt tgctgaagtg aaagaagggc
     4561 tgaacaaagc aggcctgcca gcacaggtga tgatcgattt cagccatgct aactcgtcca
     4621 aacaattcaa aaagcagatg gatgtttgtg ctgacgtttg ccagcagatt gccggtggcg
     4681 aaaaggccat tattggcgtg atggtggaaa gccatctggt ggaaggcaat cagagcctcg
     4741 agagcgggga gccgctggcc tacggtaaga gcatcaccga tgcctgcatc ggctgggaag
     4801 ataccgatgc tctgttacgt caactggcga atgcagtaaa agcgcgtcgc gggtaaggtt
     4861 taattgtcgg atgcgccgtc agagtggcgt atccgatgaa tcaccacagg cctgataagt
     4921 cgcgcagcgt cgcatcaggc aatgtgctcc attgttagca acaaaaaagc cgactcactt
     4981 gcagtcggct ttctcatttt aaacgaatga cgtttacttc gctttaccct ggtttgcaac
     5041 cgccgctgct ttcgctgcga tctcgtcagc attacccaga taatagcgtt tcagcggttt
     5101 gaaattctcg tcgaactcat acaccagcgg cacgccagtc gggatattaa gctcaagaat
     5161 ctcttcttcg ctcatgttat caagatattt caccagcgca cgtaaagagt taccgtgtgc
     5221 agcgatgatc acgcgctcac cgctcttcat acgcggcaga atagtttcat tccagtaagg
     5281 gatcacgcgg tcaatggtca gcgccaggct ttccgtcagc ggcagttctt tctcgctcag
     5341 tttcgcgtaa cgcggatcgt gacccggata acgctcatca tctttagtca gttccggcgg
     5401 agtcactgca aaaccacgac gccactgttt cacctgctcg tcgccatact tttcagcagt
     5461 ttccgctttg ttcagaccct gcaacgcacc gtagtgacgt tcgttcagtt tccaggattt
     5521 ctcaacgggc agccatgcct gatccagttc gtccagcaca ttccacaggg tatggatagc
     5581 gcgtttcagc acagaagtgt aagcaaagtc aaagctgtaa ccttcctctt tcagcagctt
     5641 acctgctgct tttgcttcgc ttacgccttt ctcagacaga tccacgtcgt accaaccggt
     5701 gaaacggttt tctttgttcc actgactttc gccatgacga accagaacca gcttagttac
     5761 agccatatac ttactcctca aatcatcttt taatgataat aattctcatt atattgccgc
     5821 gacgaagcaa cagcaatgct tacgcataac catagcgaaa atagtggcgc agtgtaaggt
     5881 tgttgtgaat attgagttgc aaatatgtcg gtgtttgctg gtgatttgaa caatatgaga
     5941 taaagccctc atgacgaggg cgtaacatta ctcagcaata aactgatatt ccgtcaggct
     6001 ggaatactct tcgccaggac gcaggaagca gtccggttgc ggccattcag ggtggttcgg
     6061 gctgtccggt agaaactcgc tttccagagc cagcccttgc cagtcggcgt aaggttcggt
     6121 tccccgcgac ggtgtgccgc cgaggaagtt gccggagtag aattgcagag ccggagcggt
     6181 ggtgtagacc ttcagctgca atttttcatc tgctgaccag acatgcgccg ccactttctt
     6241 gccatcgcct ttggcctgta acaagaatgc gtgatcgtaa cctttcactt tgcgctgatc
     6301 gtcgtcggca agaaactcac tggcgatgat tttggcgctg cggaaatcaa aagacgttcc
     6361 ggcgacagat ttcaggccgt cgtgcggaat gccgccttca tcaaccggca gatattcgtc
     6421 cgccagaatc tgcaacttgt gattgcgcac gtcagactgc tcgccgtcaa gattgaaata
     6481 gacgtgatta gtcatattca ccgggcaagg tttatcaact gtggcgcgat aagtaatgga
     6541 gatacggtta tcgtcggtca gacgatattg caccgtcgcg ccgagattac ccgggaagcc
     6601 ctgatcacca tcatctgaac tcagggcaaa cagcacctga cgatcgttct ggttcacaat
     6661 ctgccagcga cgtttgtcga acccttccgg cccgccgtgc agctggttaa cgccctgact
     6721 tggcgaaagc gtcacggttt caccgtcaaa ggtataacgg ctattggcga tacggttggc
     6781 ataacgacca atagaggccc ccagaaacgc ggcctgatcc tgatagcatt ccgggctggc
     6841 acagccgagc agcgcctcgc ggacgctgcc atcggaaagc ggaatacggg cggaaagtaa
     6901 agtcgcaccc cagtccatca gcgtgactac catccctgcg ttgttacgca aagttaacag
     6961 tcggtacggc tgaccatcgg gtgccagtgc gggagtttcg ttcagcactg tcctgctcct
     7021 tgtgatggtt tacaaacgta aaaagtctct ttaatacctg tttttgcttc atattgttca
     7081 gcgacagctt gctgtacggc aggcaccagc tcttccggga tcagcgcgac gatacagccg
     7141 ccaaatccgc cgccggtcat gcgtacgcca cctttgtcgc caatcacagc tttgacgatt
     7201 tctaccagag tgtcaatttg cggcacggtg atttcgaaat catcgcgcat agaggcatga
     7261 gactccgcca tcaactcgcc catacgtttc aggtcgcctt gctccagcgc gctggcagct
     7321 tcaacggtgc gggcgttttc agtcagtata tgacgcacgc gttttgccac gatcgggtcc
     7381 agttcatgcg caacagcgtt gaactcttca atggtgacat cacgcagggc tggctgctgg
     7441 aagaaacgcg caccggtttc gcactgttca cgacgggtgt tgtattcgct gccaaccagg
     7501 gtacgtttga agttactgtt gatgatgacg acagccacac ctttgggcat ggaaactgct
     7561 ttggtcccca gtgagcggca atcgatcagc aaggcatgat ctttcttgcc gagcgcggaa
     7621 attagctgat ccatgatccc gcagttacag cctacaaact ggttttctgc ttcctgaccg
     7681 ttaagcgcga tttgtgcgcc gtccagcggc agatgataaa gctgctgcaa tacggttccg
     7741 accgcgactt ccagtgaagc ggaagaactt aacccggcac cctgcggcac attgccgctg
     7801 atcaccatgt ccacgccgcc gaagctgttg ttacgcagtt gcagatgttt caccacgcca
     7861 cgaacgtagt tagcccattg atagttttca tgtgcgacaa tgggcgcatc gagggaaaac
     7921 tcgtcgagct gattttcata atcggctgcc atcacgcgaa ctttacggtc atcgcgtggt
     7981 gcacaactga tcacggtttg ataatcaatc gcgcagggca gaacgaaacc gtcgttgtag
     8041 tcggtgtgtt caccaatcaa attcacgcgg ccaggcgcct gaatggtgtg agtggcaggg
     8101 tagccaaatg cgttggcaaa cagagattgt gttttttctt tcagactcat ttcttacact
     8161 ccggattcgc gaaaatggat atcgctgact gcgcgcaaac gctctgctgc ctgttctgcg
     8221 gtcaggtctc gctgggtctc tgccagcatt tcataaccaa ccataaattt acgtacggtg
     8281 gcggagcgca gcagaggcgg ataaaagtgc gcgtgcagct gccagtgttg attctcttcg
     8341 ccattaaatg gcgcgccgtg ccagcccata gagtagggga aggagcactg gaagaggttg
     8401 tcataacgac tggtcagctt tttcaacgcc agcgccagat cgctgcgctg ggcgtcggtc
     8461 aaatcggtga tccgtaaaac gtgggctttg ggcagcagta gcgtttcgaa cggccaggca
     8521 gcccagtaag gcacgacggc taaccagtgt tcggtttcga caacggtacg gctaccgtct
     8581 gctagctcgc gctgaacata atccaccagc attggtgatt tctgttcggc aaaatattct
     8641 ttttgcaggc ggtcttcgcg ctcagcttcg ttaggcagga agctatttgc ccaaatctga
     8701 ccgtgcggat gcgggttaga gcagcccatc gccgcgcctt tgttttcaaa aacctgcacc
     8761 catgggtacg ttttccccag ttctgcggtt tgctcctgcc aggttttgac gatttccgtc
     8821 aatgctgcaa cgctgagctc tggcagcgtt ttactgtgat ccggtgaaaa gcagatcacc
     8881 cggctggtgc cgcgcgcgct ctggcaacgc atcagcggat cgtgactttc tggcgcatct
     8941 ggcgtgtcag acatcaaagc cgcaaagtca ttagtgaaaa cgtaagtccc ggtgtaatcg
     9001 gggtttttat cgcctgtcac ccgcacatta cctgcgcaga ggaagcaatc tggatcgtgc
     9061 gcaggtaaca cctgtttggc tggcgtttcc tgcgccccct gccaggggcg cttagcgcgg
     9121 tgcggtgaaa ccagaatcca ttgcccggtg agcgggttgt agcggcgatg tggatgatca
     9181 acgggattaa attgcgtcat ggtcgttcct taatcgggat atccctgtgg atggcgtgac
     9241 tgccagtgcc aggtgtcctg cgccatttca tcgagtgtgc gcgttacgcg ccagttcagt
     9301 tcacggtcgg ctttgctggc gtccgcccag taggccggaa ggtcgccctc gcgacgcggt
     9361 gcaaaatgat aattaaccgg tttgccgcag gctttgctga aggcattaac cacgtccagc
     9421 acgctgttgc ctacgccagc gccgaggttg tagatgtgta cgcctggctt gttcgccagt
     9481 ttttccatcg ccacgacgtg accgtccgcc agatccatta cgtggatgta atcgcgtacg
     9541 ccagtaccat cttcggtcgg ataatcgtta ccaaaaatcg ccagcgagtc gcgacggcct
     9601 acagcaacct gggcgatgta tggcatcagg ttattcggaa tgccttgcgg atcttcgccc
     9661 atatcgcccg acggatgcgc gccaaccggg ttgaagtagc gcagcagggc aatgctccag
     9721 tccggctggg ctttttgcag atcggtgagg atctgttcca ccatcagctt gcttttgccg
     9781 taagggcttt gcggtgtgcc ggtcgggaag ctttcaacgt atggaatttt gggctgatcg
     9841 ccataaacgg tggcggagga gctaaaaata aagtttttga cgttagcggc gcgcatggcg
     9901 ctaatcaggc gcagagtgcc gttgacattg ttgtcgtaat attccagcgg tttttgtacc
     9961 gattcgccca cggctttcag cccggcgaag tggatcacgg tgtcgatagc gtgatcgtgc
    10021 aggatctcgg tcatcaacgc ttcgttacga atatcgcctt caacaaacgt tggatgtttg
    10081 ccgcctaaac gctcgataac aggcagtacg ctgcgcttac tgttacagag gttatcaaga
    10141 atgatgacat catgaccgtt ttgcagtaat tgcacacagg tatgacttcc aatgtaaccg
    10201 ctaccaccgg taaccagaac tctcataatt cgctccatta ggcttatggt atgaaataac
    10261 catagcataa caaagatgcg aaaagtgtga catggaataa attagtggaa tcgtttacac
    10321 aagaatttag ccgtttttta tgcgcgatta agtgattata aaacagaggg tttatgaatg
    10381 attgcgcttt ttatctgaaa aaagacgcgg tttcatgcct gcatgcgtcg aaccgttggc
    10441 cggagagggc gctaaggccg cctccggcaa ggtcagcact accgactcaa tatatttttg
    10501 tcagcacata gcgatagagt ccaccgtccg gcacgaactc aagacgatgg gtaatacagg
    10561 caggcgcatc ttcagcgtgg tgcgaaacaa acaacaattg cgtttcacct tcgctaatca
    10621 gcacatcaac aaaacggcgg ataagctggc gattcagcgg atcaagcccc tgtagtggtt
    10681 catcgagaat aagcaacgtc ggatgtttca ccagtgcgcg gacaatcagc gccagacgct
    10741 gctgtcccca ggaaagacta tggaacggag cgtcagccgt gcgtttatca atgccgagaa
    10801 tatccagcca ctgctgcacc agtttttgct ggcgatccga aacggcctga taaatgccaa
    10861 tcgaatcaaa atagccagaa agaatcacat tacgcacggt agtgctgacc cggtaatcca
    10921 gatgcaaact actgctgacg taaccgatat gctttttgat atcccagatg gtttcgccgc
    10981 tgccgcgacg tcgtccgaaa agcgtcaaat cgttgctgta accttgcgga tgatcgccag
    11041 taaccaggct taataacgtc gattttcctg caccatttgg cccgacaatt tgccagtgtt
    11101 cgcctggatt cacctgccag ctaaggttat taagaatggg gcgatcgtta taagaaacca
    11161 cgccattgtt cagcacaatg cgcggttcgt tggcgggtaa ggcgtgacgt gctgaaggtt
    11221 catccggctc cggcagttgc acaccttcaa gctgttcact atgcgccagt tgcgcgacga
    11281 gtgcttgttg gagcagttcc tctttagcgc cagtttccgc taacgtgcaa tccgccagca
    11341 cgccagcaaa ctggacaaac tccgggatct catcgaagcg attgagcacc agtaccagag
    11401 taataccgga ctgatgtaac gaggcgagtc gctcagccag ctgctgacgt gaggcaacat
    11461 ccaggccatc gaacggctca tcaagaatca acaagtcagg ctccgacatc agcgcctgac
    11521 acagcagggt ttttcgcgtc tcgccagtgg aaaggtattt aaagcgtcgg tcgaggaggg
    11581 cggtaatacc gaactgctgc gccagttgca tgcaacgcgg tgcatccttt acttcatcct
    11641 gaatgatctc agccgtagtg cgtccggtgt catcttcgcc agggccgagc atatcggtgt
    11701 tattccgctg ccattcgtcg ctgacgagtt tttgcaattg ctcgaaggag agacgagtga
    11761 tgtgggaaaa ctggctttgc cgttcacctt tcaaaagcgg aagttccccc gccagcgcgc
    11821 gggccagggc cgatttcccg cttccattcg aaccgacaaa cgcccaacta tcacccgcgt
    11881 ttaacgttag ctgaggcaat tgcagcgttt ttgtgtcgct aagacgaaac gtgccttgca
    11941 aaatttgcaa cgatgacatt ttatatccca ctttgtgcag cgattactga cagggatacg
    12001 tgtttcataa caaattgtca acacgcttag cacagcgtgg cgataatcac gctgtcggca
    12061 ttaaagtagg ccgtgacatt ctgtccttgc tgaagagaag tcgcttcatt taccggcact
    12121 gtggcgcaca gtgtttgccc gtcgggtagc gccattaata cttcgcactg ctctgcgccg
    12181 cgctcaatat gactaataat acccggtaat tggttgtcag cgttttgcgc gaccgcctcg
    12241 tcctgagtaa tacctaccca cggcgctttt agcaatatca acacttcttt gccttcatcc
    12301 agccccagac gcgcgccgct ttgtgcggta attgcgactt tcaggcgtgt ttttccgtca
    12361 gccagtaaga catcaacatg ctgttgaacg tcatcatgat cgcgggcggt gatggtaccg
    12421 aaccactggt tacgggcgct ggtttgcagt gaaaaacgtg agatcgcggc cagcaggctg
    12481 ttcagcggca gggcgtcatc gtcacttaac acatcaaagg ctttttgctg gatttgcgcc
    12541 agtaagtcat agagctgaat cagtcgctga ccatagcggg tcagtactgc gccgccgcca
    12601 cctttaccgc ctgttgcgcg ctcgaccaga atatgctcac ttaactgatt catctcgtta
    12661 atggcatccc aggcgctttt atagctaata ccggcatctt tcgctccctg gctaatggaa
    12721 ccggaaagcg caatgtgttt tagtagcgaa atgcggcgcg ggtcggcgaa taatttttgt
    12781 tggagcttaa gggtgagaag gatttcggcc tgcataacaa tgtcctggca aaagtcttat
    12841 tgtgacggaa aacgaacgcc acgcaaagct gaccgcacaa aaggggagtg cttttctgtg
    12901 cttagcggtt agaatagtct catgactata tctggagttg accatgttag agttattaaa
    12961 aagtctggta ttcgccgtaa tcatggtacc tgtcgtgatg gccatcatcc tgggtctgat
    13021 ttacggtctt ggtgaagtat tcaacatctt ttctggtgtt ggtaaaaaag accagcccgg
    13081 acaaaatcat tgattccctg aatgcccgct tagtcgggca ttttcttttt ctcaacttcc
    13141 tgcttttcct gccgatattt tttcttatct acctcacaaa ggttagcaat aactgctggg
    13201 aaaattccga gttagtcgtt atattgtcga ctacataacg ttacattaag gggttaccaa
    13261 tggctcgtaa atggttgaac ttgtttgccg gggcggcact ctctttcgct gttgctggca
    13321 atgcactggc agatgaaggg aaaatcacgg tgttcgccgc cgcatcactg actaacgcaa
    13381 tgcaggacat tgctacgcag tttaaaaaag agaaaggcgt ggatgtggtt tcttctttcg
    13441 cttcgtcatc tactctcgcc cgtcagattg aagcgggtgc gcctgcggat ctgtttattt
    13501 ctgccgatca gaaatggatg gattatgcgg ttgataaaaa agcgatcgat acagctacgc
    13561 gtcagacact gctcggcaat agcctggtcg ttgtagcacc gaaagccagc gtgcagaaag
    13621 atttcaccat cgacagcaaa accaactgga cttcactgct gaatggcggt cgcctggcgg
    13681 ttggcgatcc ggaacatgtt cccgctggca tttatgcaaa agaagcactg caaaaactgg
    13741 gcgcatggga tacgctctct ccgaaactgg ccccagcgga agatgttcgt ggggcgctgg
    13801 cgctggtcga acgtaacgaa gcgcctctgg gcattgtcta cggttctgac gcagttgcca
    13861 gcaaaggggt aaaagtggtt gccaccttcc cggaagattc acataaaaaa gtggaatatc
    13921 cggttgctgt tgtggaaggg cataacaatg cgacagtgaa agcattttat gattatctga
    13981 agggaccgca ggcagcggaa atctttaaac gttacggatt tacaatcaag taatgatact
    14041 gaccgatcca gaatggcagg cagttttatt aagcctgaaa gtttcttccc tggctgtgct
    14101 gtttagcctg ccgtttggga tcttttttgc ctggttactg gtgcgttgca cgtttccggg
    14161 caaagctctg ctcgacagcg tactgcatct accgctggtg ttaccgcccg tggtcgtcgg
    14221 ttacttatta ttagtttcga tgggacggcg cggatttatc ggtgaacgtc tgtatgactg
    14281 gtttggtatt accttcgcct ttagctggcg cggcgcggtt ctcgctgccg ccgtcatgtc
    14341 gtttccgctg atggtgcggg caattcgtct ggcgctggaa ggggttgatg tcaaactgga
    14401 acaggccgca agaacactgg gggccgggcg ctggcgcgtt ttctttacta tcacgttacc
    14461 gctgacctta ccgggaatta ttgttggtac ggtactggct tttgctcgtt ctctcggtga
    14521 gtttggtgca accatcacct ttgtgtcgaa cattcctggt gaaacgcgaa ccattccttc
    14581 tgccatgtat accctgatcc agacccccgg cggggaaagt ggagcggcga gactgtgcat
    14641 tatttctatt gcgctggcga tgatctccct gttgatttca gaatggctgg ccagaatcag
    14701 ccgtgaacgg gcggggcgct aatcatgctg gaactgaatt tttcccagac gttgggcaac
    14761 cattgcctga ctattaatga aacgctgccc gccaatggca tcactgctat ctttggcgtc
    14821 tccggtgccg gaaaaacttc gctgattaac gccatcagtg gactgacgcg cccgcaaaaa
    14881 gggcggattg tcctcaatgg gcgggtacta aatgatgccg aaaaaggtat ctgcctgacg
    14941 ccggaaaagc gtcgcgttgg ctatgttttt caggatgcgc ggctgttccc gcattacaaa
    15001 gtgcgtggca atctgcgcta cggcatgtcg aaaagtatgg tcgatcagtt cgataagctg
    15061 gtggcgcttt taggcattga accgttgctt gaccgtttac caggcagcct gtccggaggc
    15121 gaaaaacagc gcgtggcgat tggtcgggct ttgctgacag caccggaatt gctgttgctg
    15181 gatgaaccgc tggcgtcact ggatattccg cgtaaacgcg aactgttgcc ttatctgcaa
    15241 cggctgacac gggaaatcaa cattccgatg ttgtatgtca gccattcgct ggatgagatc
    15301 ctccatctgg cagacagagt gatggtactg gaaaacggtc aggtgaaagc ctttggcgcg
    15361 ctggaggaag tgtggggcag tagcgtgatg aatccgtggc tgccgaaaga gcaacaaagt
    15421 agcattctga aagtgacggt gcttgagcat catccgcatt acgcgatgac cgcgctggcg
    15481 ctgggcgatc agcatttgtg ggtcaataag ctggacgaac cgttgcaagc tgcgctacgt
    15541 atccgcattc aggcttccga tgtttctctg gttttacaac cgccgcagca aaccagcatt
    15601 cgtaacgtat tgcgggcaaa agttgttaat agttatgacg acaacggcca ggtggaagtg
    15661 gaactggaag tcggcggtaa aacgctgtgg gcgcgtatca gcccgtgggc cagggatgaa
    15721 ctggcgatca aacctggcct gtggctgtac gcgcaaatta aaagtgtgtc gataaccgcc
    15781 tgattaaatc aggtggctat aaatgaactg ggcaatgctg tcggtggtgt tatcaccaat
    15841 cacaatgttg gcgcgcgctt ttaccgcgtc atcggcgttg cccatcgcca cgcctgtacc
    15901 agcggcttcc agcatactga tatcattaaa gttatcgccg aatgccacga cgttttccat
    15961 cgaccaacct tgcgcctcaa cccatttcgt caaacgttta cctttgctgt tgccgccgcg
    16021 tgcaatatca acctgatcgt gccaggacca ttcacactcc agtcccagtt catgttcgac
    16081 atgcttacca aaatgctgca attgcggcag gtcatcgtgc gtcagggcga acttccatac
    16141 ggcgttaact tgttgcgccg tttcagccag agaagcgact tgtgtgaaag tcggacgctg
    16201 ttccggcggc agggtttgcg cccagttaga tgtgcgaatg acatgcccgg tcgggtgctc
    16261 atagaccatt gcatcatcga catacatcag accgtgaatg tggtgttcat tcagcatctc
    16321 aatgagttgc agggctttaa taacgggcat tgggtccgct tccagcacgg tttttgcatg
    16381 ataatcatac aaataggtgc cattacagca aatagcaggt gtatccagcg ccagcgcctg
    16441 ataaaaagga tgaatagcga cgtgatggcg acctgtgacg atgattaatt gatagcctgc
    16501 ttcgcgagcg cgggccaggg cttctatcga tgaaggaagc agggtctttt tcggggtcaa
    16561 taaggtgccg tctaagtcga gagcaatcac gcgtgtggtc atggcgtatt ccagattaag
    16621 gttaagaatt ttcgtctgcg cgaatggtac accgatacca ctatcggaca aaattctgcg
    16681 ttttaattca gcattcaccg ccaaaagcga ctaattttag ctgttacagt cagttgctaa
    16741 atgcaaagga gcattcatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat
    16801 tcacgtctgg aatctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc
    16861 ggggcaggtg cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg
    16921 ccctgagttt cgcgtcctgg cgtatcgtat cgccccggac gatggcgcac tgacctttgc
    16981 cgcagagtct gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt
    17041 tgtctttgta ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct
    17101 gccagtgggc gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc
    17161 accggacaac cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac
    17221 ggtcagcgat gatggtcatc tcgtggcgca ggaccctgcg gaagtgacca ccgttgaagg
    17281 ggccggcccg cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga
    17341 gttaaacagc tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt
    17401 ccagacgctg gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca
    17461 tatcaccccg gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt
    17521 tttcagcgtt tcggaagatg gcagcgtgtt gagtaaagaa ggcttccagc caacggaaac
    17581 ccagccacgc ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa
    17641 atctcaccac atctcggtat acgaaattgt tggcgagcag gggctactgc atgaaaaagg
    17701 ccgctatgcg gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat
    17761 ttacccggcg cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcgtgtctc
    17821 tggcgttcga cgatattggt ccacaaattg tctttatcgt cagtccataa attaatcagc
    17881 aaggcaaaaa agcgttctgc tgctggagaa agtacggcat ctttacggcg aattattccc
    17941 aatgttcgac gtataacggg ttccaccaat gggataccaa taatcgaaga atagggcgca
    18001 tgaggcattg ccaggccagg aagcgccgaa atccccagtc ccgcctccac cagtcctaat
    18061 gacgtcgaaa gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc
    18121 tgttgttcta tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc
    18181 actaactcct gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggcc
    18241 agcacgaacg gctcgttgac cagtggggta aaatcaatgg atgaatttgt gacgttgttc
    18301 atgtttatgc caaagtctga ttcgttacac agcaccgatt ccatgcaatt attcgtgcct
    18361 tgttccagaa tccgcacttt aatattggga tacagctcat taaatttacc gattgccagc
    18421 ggtaaaaaat aaaatactgc ggtcggaata cacgctaacg tcaccatacc acgatgataa
    18481 gcattcatat cacgaatatt aaaaagcgtt tcatcgaatt tctttattaa ttcccgcgcc
    18541 tccgggagca acctttttcc tgcttttgtt aacgtaacct tgcgagttgt acgctcaaat
    18601 agttgaatat gtaaatcttc ctccatcttt tttattcttc gcgttaatgc aggttgcgta
    18661 atattgagta atttagcggc attgttaaac gaactggact ctgccagtat gacaaatgcc
    18721 ttcatacttg ataattcatg cttcattatt actccggaaa atggaagcga cgattttggg
    18781 tggctggccg ttaaaaattt taactgcatt tagccaactt aaattaatga aaaaatgtta
    18841 ttaatcgttg agctaaagtc attagagatg ctttgccctt aatgtaacca tatcgcaata
    18901 agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag
    18961 gtggaacctc gaggggcgcg ttcctgttag cggaacattt acccgaagat caaacgcagc
    19021 gcgataaaat attgatggca attatgggtt ccggtaacga tctggaaatt gacggtattg
    19081 gcggcggtaa tccactgacc agtaaagtcg ccattattag ccgttccagc gatccgcgtg
    19141 ctgatgtcga ttatctgttt gcgcaggtaa tcgttcatga gcaacgtgtc gataccacgc
    19201 ctaactgcgg caatatgctg tctggtgttg gggcattcgc cattgaaaat ggtttgattg
    19261 cagcgacttc gccagttact cgcgtacgta tccgcaacgt caatacgggt acgttcatcg
    19321 aagctgatgt gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc agaattgacg
    19381 gcgtaccggg tactgccgca ccggttgcgc tcactttcct gaatgccgct ggaaccaaaa
    19441 ccggaaaagt tttcccgact gataatcaga ttgattattt tgacgatgtc ccggtgacct
    19501 gtatcgatat ggcgatgcca gtcgtcatta ttccggctga atatctggga aaaacaggtt
    19561 atgaattacc ggcggaactg gatgccgaca aagcattatt agcccgcatt gaatctatcc
    19621 gtctacaagc gggtaaagca atgggcttag gtgatgtcag taatatggtt atccctaaac
    19681 ctgtgcttat ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat tttatgccgc
    19741 attcttgcca tcgcgcgctg gcgataaccg gtgctattgc tatttccagt agttgtgcat
    19801 tggaaggcac cgtcacccga caaatcgtcc cttctgtagg atacggcaat atcaatattg
    19861 aacaccccag tggtgcgctc gacgttcatt taagtaatga aggtcaggat gccacgacgt
    19921 tacgcgcatc tgttattcgg acgaccagaa aaatattttc cggtgaagtt tatcttccct
    19981 gaaaaaattc gttgtcagga taaggacaat caataaagga cttctgtatg agtcatacag
    20041 aaagaacagg attttaaatg aataagaaat cgttatggaa gctaattctg atattagcga
    20101 tcccatgtat tattggtttt atgccagctc cggcaggatt aagcgaactg gcgtgggtgc
    20161 tttttggtat ttacctggcg gccattgtgg ggctggttat caagcctttc ccggaacctg
    20221 tcgtactgtt aattgccgtt gctgcctcaa tggtggtggt cggtaactta tccgacggtg
    20281 cgtttaaaac caccgccgta ttaagcggtt actcttcagg taccacctgg ctggtgttct
    20341 cggcgtttac cttaagcgcc gcatttgtga ccaccggttt aggtaaacgt attgcctatc
    20401 tgctgattgg taaaatcggt aacaccacgc tgggtctggg ttacgttacg gtattcctcg
    20461 atctggtact ggctccggca acaccgtcta acaccgcgcg tgcgggcggc attgtgttac
    20521 cgatcatcaa cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt ccgcgtcgtg
    20581 tcggacatta cctgatgatg tccatttaca tggtcaccaa aaccaccagc tatatgttct
    20641 ttaccgcaat ggcggggaac attctggcgc tgaaaatgat caacgacatt ctgcacctgc
    20701 aaattagctg gggtggatgg gcgctggcag ccggattgcc gggcatcatt atgctgctgg
    20761 tcaccccgct ggtgatttac accatgtatc caccagaaat taagaaggtg gataacaaaa
    20821 ccatcgctaa agcgggcctt gccgaactag gaccgatgaa aatccgcgaa aaaatgctgc
    20881 tcggtgtctt tgtgctggcg ctgctgggct ggattttcag taagtctctg ggggttgatg
    20941 aatccaccgt ggcaatcgtt gttatggcaa ccatgctgct gctgggtatc gttacctggg
    21001 aagacgtggt taaaaataaa ggcggctgga ataccttaat ctggtacggc ggtattatcg
    21061 gcttaagctc cttattatcg aaagttaaat tcttcgaatg gttagctgaa gtctttaaaa
    21121 ataacctggc atttgatggt cacggtaacg ttgctttctt cgttattatt ttcctcagca
    21181 ttatcgtgcg ttatttcttc gcttccggta gtgcctatat cgttgctatg ttaccggtat
    21241 ttgccatgct ggcgaacgtc tccggcgcac cgttaatgtt aaccgcgctg gcactgttgt
    21301 tctccaactc ctatggcggc atggttactc actatggcgg cgcggcaggt ccggtcatct
    21361 ttggcgtggg ttataacgat attaaatcct ggtggttggt cggtgcggta ctgacgatat
    21421 taaccttcct ggtgcatatc accctcggcg tgtggtggtg gaatatgctg atcggctgga
    21481 acatgctgta aatattctcg tcatacttca agttgcatgt gctgcgtctg cgttcgctca
    21541 ccccagtcac ttacttatgt aagctcctgg ggattcactc gcttgtcgcc ttcctgcaac
    21601 tcgaattatt tagagtatat ccatttatta tctttctgcg cacttcacgg tgcgcagata
    21661 tctggagcat ttgatgatca agttatctga aaaaggcgtg tttctcgcca gtaataacga
    21721 aataattgcc gaagaacatt tcaccggcga aattaaaaaa gaagaagcca aaaaaggcac
    21781 tattgcctgg tctattctct cttcgcataa tacgtccgga aatatggata aacttaaaat
    21841 taagtttgat tcattagcct ctcacgatat tacctttgtt ggtattgtac agaccgctaa
    21901 agcgtccggt atggaacgtt tcccgctgcc gtatgtgctg accaactgcc ataactcact
    21961 ctgcgccgtc ggcggcacta ttaacggtga tgaccatgtt tttggtttat cggcggccca
    22021 gcgttatggc ggtatttttg tgcctccgca tattgcggtc atccatcaat atatgcgtga
    22081 gatgatggca ggcggcggca aaatgatcct cgggtcagac agccacaccc gttacggtgc
    22141 attagggaca atggcagtcg gtgagggcgg cggtgagttg gtaaaacagc tgcttaatga
    22201 cacctgggat atcgactatc cgggcgtggt tgcggtgcat ctgaccggaa aacctgcgcc
    22261 gtatgtgggg ccacaggatg tggcgctggc tatcattggc gcggtgttca aaaacggtta
    22321 cgtcaaaaac aaagtcatgg agttcgttgg accgggcgtt agcgcgctct ctaccgattt
    22381 ccgtaacagc gttgacgtga tgaccactga aacgacctgt ttaagttctg tctggcaaac
    22441 cgatgaagaa gtccataact ggctggcgct gcacggtcgc ggccaggatt actgccagct
    22501 taaccctcaa ccgatggcgt actacgatgg ctgcatcagc gttgatttaa gcgccatcaa
    22561 accaatgatt gcgctgccgt tccacccgag caacgtgtat gaaatcgaca cactgaacca
    22621 gaacctgacc gacattctgc gtgagattga aattgagtcc gaacgcgtgg cgcacggtaa
    22681 agccaaactc tcgctgctgg ataaagtgga aaatggtcgc ctgaaagtgc agcaggggat
    22741 tatcgcgggc tgttctggcg gtaactacga aaacgtcatc gcggcggcga atgcactgcg
    22801 cggtcaatcc tgtggcaatg acaccttctc gctggcagtt tacccgtcat cacagccggt
    22861 gtttatggat ctcgccaaaa aaggtgtggt agcagatttg attggcgcag gcgcaatcat
    22921 cagaaccgcg ttctgcggcc catgctttgg cgcgggcgat acgccaatca acaacggttt
    22981 gagtattcgc cacaccacgc gtaacttccc gaaccgcgaa ggctctaagc cagctaatgg
    23041 gcagatgtca gcggtggcgt tgatggacgc tcgttctatc gctgcgactg cggcaaacgg
    23101 tggctattta acctctgcca gcgaacttga ttgctgggac aacgtgccgg agtacgcctt
    23161 cgatgtaacg ccgtataaaa accgtgttta tcagggcttt gtgaaagggg caactcagca
    23221 accgctgatt tacgggccga acattaaaga ctggccggaa ttgggtgcgc tgactgacaa
    23281 tatcgtcctg aaagtgtgct cgaagatcct cgacgaagtg accaccaccg acgaactgat
    23341 tccttccggt gaaacctctt cttatcgttc aaatccgatt ggtctggcgg agtttaccct
    23401 gtctcgccgc gatcccggtt atgttagcag aagtaaagcg actgctgagc tggaaaatca
    23461 gcgtctggcg gggaatgtca gcgagctgac agaggtgttt gcgcgcatta agcagattgc
    23521 tggtcaggag catattgatc cgctgcaaac tgaaattggc agcatggtct atgcggtgaa
    23581 accaggcgat ggttctgcgc gtgaacaggc ggcgagctgc cagcgtgtga ttggcggtct
    23641 ggcgaatatt gccgaggagt acgcgactaa acgctatcgt tctaacgtca tcaactgggg
    23701 gatgttaccg ctgcaaatgg cggaagtacc aacctttgaa gtgggggatt acatttacat
    23761 ccctggcatt aaagcggcgc tggataatcc gggtacgacg tttaaaggtt atgtgatcca
    23821 tgaagatgcg ccggtaacgg aaattacgct ctatatggaa agtctgactg ctgaagagcg
    23881 cgagattatc aaggcgggta gtttgattaa cttcaataaa aaccgtcaga tgtaaaaagc
    23941 gccatgtgaa tgtaggtcgc attcggcact tattgtcgga tgcgatgctt gcgcatctta
    24001 tccgacctac gaatcgcatc gaatctgtag gccagataag gcattttcgc agcatccggc
    24061 acttattgtc ggatgcgatg cttgcgcatc ttatccgacc tacaaatcgc atcgaaccgt
    24121 aggccggata aggcgtttac gccgcatccg gcaaatagtt aattgctctt acttcttcgc
    24181 ctctgcaacc actttactac ccacgccgcg gttattgtat tcccacatgc ggttgtagtt
    24241 agtgtcattc agattgcgct gtatttcgtc gttatcatct acgctgccgg tattacccgc
    24301 aaacggacga ttagagatca ccgcatcggc ccacggttta gccgtgttaa aaccttcgtt
    24361 gatggcgcta tcacggatca ccacctgacc gttggtattg gcatcaacat ccagcgagcg
    24421 gcccagttgc gccacaccat caccgaaagc attgaaacgg ctgtttacgg cgaggaaacc
    24481 gtagtaaatg ttggacagcg tagccggtgc aaacacatac gcttcttgct gagtacgtga
    24541 gttcaccacg cggaattcgg tgttatcgaa caccactgcg ccgcgaccag aaacgatatc
    24601 cacatcccct tcaatgtagc tgttggtcac cagcgtacgc ggctgacgat tcgtttccag
    24661 acggttctgc acaccgctgt tggtgacaaa gaaggtgttc tgacgaccga gaatgttaac
    24721 gttgttaatc tgtacctggt caccatcagt acgcagtgcc accgccggat ggttacctgc
    24781 atctacgcta tcgcccagcg tgttttcgat ggtcagattt tgcagttgca ggccattgtt
    24841 ttgtgaccag aagaccgcag agcagagaac accgatactg tcgctgcgtt tgctctggca
    24901 gctatcgtac atataccacg ctggtttacc tggcatatat ttgccgcgcg ggttgacgtc
    24961 gtgacgccag tcggcagggc tcatgccacc atcaagggaa agcccaatct tcacatcaat
    25021 cggtttttca cctgtaccgt acagagtaat tccacccgga gcggcaggga catataccgt
    25081 tccctgatac tcaccaggca tcacggcaat atactggcgc ttgttggtac gcttgataat
    25141 tgccgcatct accgccgcct gaatcgtggt atgcgttaca ccttgagtgc ccgccgggcc
    25201 gacaacaaag tcaggttgcg caggcagggt aatcggggaa ggattccacg ctgcagcacc
    25261 tggtgtcagg gatgcaaaat agtgttgagc atcgaaattc tgcgcttctt ttgccgacag
    25321 aatcgggcga gaagaggtac caggcgcggt ttgatcagaa ggacgttgat cgggcggggt
    25381 tgagctacag gcggtcagcg tcacgccaaa agccaatgcc agcgccagac gggaaactga
    25441 aaatgtgttc acaggttgct ccgggctatg aaatagaaaa atgaatccgt tgaagcctgc
    25501 ttttttatac taacttgagc gaaacgggaa ggtaaaaaga caaaaagttg tttttaatac
    25561 ctttaagtga taccagatgg cattgcgcca tctggcagag tgattaacta aacatcgcag
    25621 taatcgaggc gcttgccaga gagtggaaat gaacgttaaa cccgaccatc gcgccgctgg
    25681 caccttcatc gacatcaata cgttctatat ccagcgcgtg aacggtaaaa atgtagcgat
    25741 gagtttcgcc tttcggcggt gctgcgccat cgtacccggt tttaccaaag tcggtacgcg
    25801 tctgcaaaac gccgtctggc attgctacca gaccagagcc aaacccttgc ggtaatacgc
    25861 gggtatcagc gggtaagtta acaactaccc agtgccacca gccggagccg gttggcgcat
    25921 ccgggtcgta gcaggtgaca acaaaacttt tcgttcccgc aggaacatca tcccacgcca
    25981 gatgcggtga aatattatcg ccatcgtaac ccatgccgtt aaagacatga cgatgcggca
    26041 atttatcgcc atcgcgcaga tcgttactga tgagtttcat gaaccctcct ttcttgtttg
    26101 cagaaagtgt agccagaaac cctcacgcgg acttctcgtt attggcaaaa aaatgtttca
    26161 tcctgtaccg cgcggttaac cgctgcggtc agacgctgca actgttgcgg gagaataata
    26221 tagggcggca tcaggtaaat cagtttgcca aaaggccgga tccagacacc ctgttcgaca
    26281 aagaattttt gcagcgccgc catattcacc ggatgagtgg tttcgaccac gccaatggcc
    26341 cccagtacgc gcacatcggc aaccatttcg gcatcacggg cgggggcaag ttgctcgcgc
    26401 agctgtactt caatatccgc cacctgttgc tgccagtcgc cagattcgag aatcgccagg
    26461 ctggcgtttg ctgccgcgca ggccagcgga ttgcccataa aagttggccc atgcataaag
    26521 caaccggctt caccgttact gatggtttct gcaacctcgc gcgtggtgag tgtggcggaa
    26581 agggtcattg tgccgccggt taaggcttta ccgaggcaca aaatgtccgg cgcgatttct
    26641 gcatgttcac aggcaaacag tttcccggta cgaccaaatc cagtggcgat ctcgtcggca
    26701 atcagcaaga taccttcgcg atcgcatatt ttgcggattc gttttaacca ttccggatgg
    26761 tacatgcgca tcccgcctgc gccctggaca atcggctcaa tgatcaccgc cgcgatttca
    26821 tgacgatgcg ccgccatcag gcgggcaaag cccaccatat cgcgctcatc ccattcgcca
    26881 tccatgcggc tttgcggggc gggagcaaac aggttttctg gcaggtagcc tttccacaga
    26941 ctgtgcattg agttatccgg atcgcacacc gacatcgcgc caaaggtatc gccatgataa
    27001 ccattgcgga aggtcagaaa acgctggcgc gcttcgcctt tggcttgcca gtactgcaac
    27061 gccattttca tcgccacttc caccgctacg gaaccggagt ccgcgagaaa aacgcactcc
    27121 agcggttgcg ccgtcatcgc caccagtttg cggcacagct caatggctgg cgcatgggtg
    27181 ataccgccaa acatcacatg cgacatggca tcaatttgcg acttcatcgc cgcattaagc
    27241 tgcgggtgat tgtagccgtg gatcgccgcc caccaggacg acataccgtc aaccaggcgt
    27301 ctgccgtcag acaaaatcag ctcgcaacct tcggcgctca ccaccggata aaccggcaga
    27361 ggggaggtca tggatgtgta tgggtgccag atatggcgtt ggtcaaaggc aagatcgtcc
    27421 gttgtcataa tcgacttgta aaccaaattg aaaagattta ggtttacaag tctacaccga
    27481 attaacaaca aaaaacacgt tttggagaag ccccatggct caccgcccac gctggacatt
    27541 gtcgcaagtc acagaattat ttgaaaaacc gttgctggat ctgctgtttg aagcgcagca
    27601 ggtgcatcgc cagcatttcg atcctcgtca ggtgcaggtc agcacgttgc tgtcgattaa
    27661 gaccggagct tgtccggaag attgcaaata ctgcccgcaa agctcgcgct acaaaaccgg
    27721 gctggaagcc gagcggttga tggaagttga acaggtgctg gagtcggcgc gcaaagcgaa
    27781 agcggcagga tcgacgcgct tctgtatggg cgcggcgtgg aagaatcccc acgaacgcga
    27841 tatgccgtac ctggaacaaa tggtgcaggg ggtaaaagcg atggggctgg aggcgtgtat
    27901 gacgctgggc acgttgagtg aatctcaggc gcagcgcctc gcgaacgccg ggctggatta
    27961 ctacaaccac aacctggaca cctcgccgga gttttacggc aatatcatca ccacacgcac
    28021 ttatcaggaa cgcctcgata cgctggaaaa agtgcgcgat gccgggatca aagtctgttc
    28081 tggcggcatt gtgggcttag gcgaaacggt aaaagatcgc gccggattat tgctgcaact
    28141 ggcaaacctg ccgacgccgc cggaaagcgt gccaatcaac atgctggtga aggtgaaagg
    28201 cacgccgctt gccgataacg atgatgtcga tgcctttgat tttattcgca ccattgcggt
    28261 cgcgcggatc atgatgccaa cctcttacgt gcgcctttct gccggacgcg agcagatgaa
    28321 cgaacagact caggcgatgt gctttatggc aggcgcaaac tcgattttct acggttgcaa
    28381 actgctgacc acgccgaatc cggaagaaga taaagacctg caactgttcc gcaaactggg
    28441 gctaaatccg cagcaaactg ccgtgctggc aggggataac gaacaacagc aacgtcttga
    28501 acaggcgctg atgaccccgg acaccgacga atattacaac gcggcagcat tatgagctgg
    28561 caggagaaaa tcaacgcggc gctcgatgcg cggcgtgctg ccgatgccct gcgtcgccgt
    28621 tatccggtgg cgcaaggagc cggacgctgg ctggtggcgg atgatcgcca gtatctgaac
    28681 ttttccagta acgattattt aggtttaagc catcatccgc aaattatccg tgcctggcag
    28741 cagggggcgg agcaatttgg catcggtagc ggcggctccg gtcacgtcag cggttatagc
    28801 gtggtgcatc aggcactgga agaagagctg gccgagtggc ttggctattc gcgggcactg
    28861 ctgtttatct ctggtttcgc cgctaatcag gcagttattg ccgcgatgat ggcgaaagag
    28921 gaccgtattg ctgccgaccg gcttagccat gcctcattgc tggaagctgc cagtttaagc
    28981 ccgtcgcagc ttcgccgttt tgctcataac gatgtcactc atttggcgcg attgcttgct
    29041 tccccctgtc cggggcagca aatggtggtg acagaaggcg tgttcagcat ggacggcgat
    29101 agtgcgccac tggcggaaat ccagcaggta acgcaacagc acaatggctg gttgatggtc
    29161 gatgatgccc acggcacggg cgttatcggg gagcaggggc gcggcagctg ctggctgcaa
    29221 aaggtaaaac cagaattgct ggtagtgact tttggcaaag gatttggcgt cagcggggca
    29281 gcggtgcttt gctccagtac ggtggcggat tatctgctgc aattcgcccg ccaccttatc
    29341 tacagcacca gtatgccgcc cgctcaggcg caggcattac gtgcgtcgct ggcggtcatt
    29401 cgcagtgatg agggtgatgc acggcgcgaa aaactggcgg cactcattac gcgttttcgt
    29461 gccggagtac aggatttgcc gtttacgctt gctgattcat gcagcgccat ccagccattg
    29521 attgtcggtg ataacagccg tgcgttacaa ctggcagaaa aactgcgcca gcaaggctgc
    29581 tgggtcacgg cgattcgccc gccaaccgta cccgctggta ctgcgcgact gcgcttaacg
    29641 ctaaccgctg cgcatgaaat gcaggatatc gaccgtctgc tggaggtgct gcatggcaac
    29701 ggttaataaa caagccattg cagcggcatt tggtcgggca gccgcacact atgagcaaca
    29761 tgcagatcta cagcgccaga gtgctgacgc cttactggca atgcttccac agcgtaaata
    29821 cacccacgta ctggacgcgg gttgtggacc tggctggatg agccgccact ggcgggaacg
    29881 tcacgcgcag gtgacggcct tagatctctc gccgccaatg cttgttcagg cacgccagaa
    29941 ggatgccgca gaccattatc tggcgggaga tatcgaatcc ctgccgttag cgactgcgac
    30001 gttcgatctt gcatggagca atctcgcagt gcagtggtgc ggtaatttat ccacggcact
    30061 ccgcgagctg tatcgggtgg tgcgccccaa aggcgtggtc gcgtttacca cgctggtgca
    30121 gggatcgtta cccgaactgc atcaggcgtg gcaggcggtg gacgagcgtc cgcatgctaa
    30181 tcgcttttta ccgccagatg aaatcgaaca gtcgctgaac ggcgtgcatt atcaacatca
    30241 tattcagccc atcacgctgt ggtttgatga tgcgctcagt gccatgcgtt cgctgaaagg
    30301 catcggtgcc acgcatcttc atgaagggcg cgacccgcga atattaacgc gttcgcagtt
    30361 gcagcgattg caactggcct ggccgcaaca gcaggggcga tatcctctga cgtatcatct
    30421 ttttttggga gtgattgctc gtgagtaaac gttattttgt caccggaacg gataccgaag
    30481 tggggaaaac tgtcgccagt tgtgcacttt tacaagccgc aaaggcagca ggctaccgga
    30541 cggcaggtta taaaccggtc gcctctggca gcgaaaagac cccggaaggt ttacgcaata
    30601 gcgacgcgct ggcgttacag cgcaacagca gcctgcagct ggattacgca acagtaaatc
    30661 cttacacctt cgcagaaccc acttcgccgc acatcatcag cgcgcaagag ggcagaccga
    30721 tagaatcatt ggtaatgagc gccggattac gcgcgcttga acaacaggct gactgggtgt
    30781 tagtggaagg tgctggcggc tggtttacgc cgctttctga cactttcact tttgcagatt
    30841 gggtaacaca ggaacaactg ccggtgatac tggtagttgg tgtgaaactc ggctgtatta
    30901 atcacgcgat gttgactgca caggtaatac aacacgccgg actgactctg gcgggttggg
    30961 tggcgaacga tgttacgcct ccgggaaaac gtcacgctga atatatgacc acgctcaccc
    31021 gcatgattcc cgcgccgctg ctgggagaga tcccctggct tgcagaaaat ccagaaaatg
    31081 cggcaaccgg aaagtacata aaccttgcct tgttgtagcc attctgtatt tggttaaatt
    31141 gcgagcgaga tcgcgtcttc gattgactgc aatttaacca attaaattct aaaataatca
    31201 cgaaaaaaat tttacttccg cctcatgcgg cgaatgtggg aattgcccag gcggcggggg
    31261 ataggggctg gagacagtta tccactattc ctgtggataa ccatgtgtat tagagttaga
    31321 aaacacgagg caagcgagag aatacgcggc ttgcacgcga attggcgtta aagacggctc
    31381 aaagaaatat cttttatttt ttaactggtt agataaatgc aatggcagtc actgaacagg
    31441 catctcttgc cataaaactg tcatcactca tcttgacaaa tgttaaaaaa gccgttgctt
    31501 tggggataac ccggtaaggc cggagtttta tctcgccaca gagtaaattt tgctcatgat
    31561 tgacagcgga gtttacgctg tatcagaaat attatggtga tgaactgttt ttttatccag
    31621 tataatttgt tggcataatt aagtacgacg agtaaaatta catacctgcc cgcccaactc
    31681 cttcaggtag cgactcatga gtaaaccgtt caaactgaat tccgctttta aaccttctgg
    31741 cgatcagcca gaggcgattc gacgtctcga agaggggctg gaagatggcc tggcgcacca
    31801 gacgttactt ggcgtgactg gctcagggaa aaccttcacc attgccaatg tcattgctga
    31861 ccttcagcgc ccaaccatgg tacttgcgcc caacaaaacg ctggcggccc agctgtatgg
    31921 cgaaatgaaa gagttcttcc cggaaaacgc ggtggaatat ttcgtttcct actacgacta
    31981 ctatcagccg gaagcctatg taccgagttc cgacactttc attgagaaag atgcctcggt
    32041 taacgaacat attgagcaga tgcgtttgtc cgccaccaaa gcgatgctgg agcggcgtga
    32101 tgtggttgtg gtggcgtctg tttccgcgat ttatggtctg ggcgatcctg atttatatct
    32161 caagatgatg ctccatctca cggtcggtat gattatcgat cagcgcgcga ttctgcgccg
    32221 actggcggag ctgcaatacg ctcgtaatga tcaagcattc cagcgtggta ctttccgcgt
    32281 tcgtggcgag gtgatagata tcttcccggc agaatcggat gacattgcac ttcgcgtgga
    32341 actgtttgac gaggaagtgg aacgattgtc gttatttgac ccgctgaccg ggcagattgt
    32401 ttccactatt ccacgtttta ccatctaccc gaaaacgcac tacgtcacac cgcgcgagcg
    32461 catcgtacag gcgatggagg agatcaaaga agagctggcc gccagacgca aagtgctgtt
    32521 ggaaaacaac aaactgctgg aagagcagcg gctgacccag cgtacccagt ttgatctgga
    32581 gatgatgaac gagctgggct actgttcggg gattgaaaac tactcgcgct tcctctccgg
    32641 tcgtggaccg ggtgagccac cgccgacgct gtttgattac ctgcctgccg atgggctgct
    32701 ggtcgtcgat gaatctcacg tcaccattcc acaaattggc ggcatgtatc gcggtgaccg
    32761 ggcgcgtaaa gagacactgg tggagtacgg cttccgcctg ccatcagcgc tggataaccg
    32821 tccgcttaag tttgaagagt tcgaagcatt agcgccgcaa accatctatg tttcggcgac
    32881 gccgggtaat tacgagctgg aaaaatccgg cggcgatgtg gtggatcagg tggtgcgtcc
    32941 aaccggattg cttgacccga ttatcgaagt gcggccggtg gcgacacagg ttgatgatct
    33001 tctttcggag attcgtcagc gagcggcaat taacgaacgc gtactggtca ccacactgac
    33061 caagcggatg gcggaagatc ttaccgaata tctcgaagaa catggcgagc gcgtgcgtta
    33121 tcttcactca gatatcgaca ccgtcgaacg tatggagatt atccgcgact tgcgtctggg
    33181 tgagttcgac gtgctggtag ggatcaactt actgcgcgaa ggtctggata tgccggaagt
    33241 gtcgctggtg gcgatcctcg acgctgacaa agaaggcttc ctgcgttccg aacgttcgtt
    33301 gatccagacc attggtcgtg cggcacgtaa cgttaacggt aaagcgattc tctacggcga
    33361 taagatcacc ccatcaatgg cgaaagcgat tggcgaaacc gaacgtcgcc gtgagaaaca
    33421 gcagaagtac aacgaggaac acggaattac gccgcaaggc ttgaacaaga aagtggtcga
    33481 tatcctggcg ctggggcaga acattgccaa aaccaaagcg aagggcagag gaaaatcgcg
    33541 cccgattgtt gagccggata atgtgccgat ggatatgtcg cctaaagcgt tgcagcagaa
    33601 aatccatgag ctggaagggt tgatgatgca acacgcgcag aatctggagt tcgaagaagc
    33661 ggcgcaaatt cgtgaccagt tgcatcagct gcgtgagctg tttatcgcgg catcgtaaca
    33721 ggatagcgaa gaagactgat gacaaacgga aaacagcctg atgcgctacg cttatcaggc
    33781 ctacattttc tccgcaatat attgaatttg cgcggtttgt aggccggata aggcgttcac
    33841 gccgcatccg gcataaacaa cgcgcacttt gtcttcaata ggaagccgga attttccttc
    33901 ggatttccgt taacctaaag cctgtaacgc cttttccagc gcgttatgta acaactggcg
    33961 gtcatgacga tacggaatat cgctggcctc cagtacctcc tggatcacaa tccgctcttt
    34021 caccgccgag acatccactt ttggcccgac gatgaccgca tcaatgacct ttttaccaac
    34081 atactgctcc ataattgcca gcttgctttc cagcttcaaa ttagccgcag gtaaactcaa
    34141 ctcacgcccc agattgccga tataaaccat cggcgctggc gtgcggcgta atgcctgggc
    34201 gatttccttc agcagcagaa ttggcatcag gctggtataa aaactgccag gcccaataat
    34261 gatgagatcc gcttcattga tagcgtgaac cgcctcacgc gttgcgggta cattaggcgt
    34321 taacaataac tcttgaatcg gcgtagttaa ctggtcgata ttgacctcgc cgtaaacttc
    34381 atgcccctga tcgtcaatcg ccatcagatc aacaggatgc tctgacattg gaatcaaatg
    34441 cgtatccact ttcagcagat tacgaattaa attgatggct tccagaggcc gcacgctaag
    34501 gtgatccagc gcctttaaca tcaagtttcc gagattatga ccggaaagtt cgccattgcc
    34561 accaaaacgg tattcaaaca tcgcggaggc gacgctcggt tccgttatca gctggttgag
    34621 gcagttgcgc atatcgcccc aggcaatgcc gccttctgaa cggcgaatac gccccgtcga
    34681 gccaccatta tcggtggtgg tgacgatacc cgttaaacga gaacccaaag acgaaagtga
    34741 tgagagaacg cgtcccagtc catgccctcc gccgagagca acgacacgat caagatcagc
    34801 cagcgtacga ttgcgcatat agtttcctgg agtcagatta tccgcgctac agtagcgcaa
    34861 attgcgggga aacagcaatt aacctgccaa tattgaggat acaaactatt ttcttctacc
    34921 tctaaaggac gatgcacgct atgcctccct gatgatgtat atcaaagtaa aaccgccatt
    34981 ttcccttatt ctgtagcgaa atagcacgat catgacgcta tatacatgat tacatagcga
    35041 aagtgtggat ggtaaaaatc tcatttacac gctagtatcg gcataaccac taaacactct
    35101 agcctctgca cctgggtcaa ctgatacggt gctttggccg tgacaatgct cgtaaagatt
    35161 gccaccaggg cgaaggaaga aatgacttcg cctcccgtat ctggaaaggt gtacatggct
    35221 tcacaactga ctgatgcatt tgcgcgtaag ttttactact tgcgcctgtc gattaccgat
    35281 gtgtgtaact ttcgttgcac ctactgcctg ccggatggct acaaaccgag cggcgtcacc
    35341 aataaaggct ttcttaccgt cgatgaaatt cgccgggtta cgcgcgcctt cgccagtctg
    35401 ggcaccgaaa aagtgcgcct gacaggagga gagccgtctt tacgccgcga ctttaccgat
    35461 atcatcgccg ctgtgcggga aaacgacgct atccgccaga ttgcggtcac aaccaatggt
    35521 taccgtctgg aacgcgatgt ggcgagctgg cgcgatgcgg gacttactgg cattaacgtc
    35581 agtgtcgaca gtctggacgc ccgccagttt cacgctatta ccgggcagga taaattcaac
    35641 caggtcatgg cagggattga tgctgcattt gaggccggtt ttgagaaggt caaagtcaat
    35701 accgtgctga tgcgtgatgt taatcatcac cagctcgaca cctttctgaa ctggatccag
    35761 catcgcccta tccagctgcg tttcatcgaa ctgatggaaa cgggcgaggg cagcgagctc
    35821 ttccgtaagc atcacatctc tggtcaggtt ctgcgtgacg agctactgcg tcgcggctgg
    35881 atccaccaat tacgtcaacg cagcgacggt cccgcgcaag tcttttgcca tccagattac
    35941 gccggagaga ttggccttat catgccgtat gaaaaagact tctgcgccac ttgcaaccgc
    36001 ctgcgcgttt cctccattgg taaactccat ctctgcctgt ttggtgaagg cggcgttaac
    36061 ctgcgcgatc tgctggaaga cgatacccag caacaggcgc tggaagcgcg tatttcagcg
    36121 gcgctgcggg agaagaaaca gacccatttc ctgcatcaaa acaacaccgg tattacgcaa
    36181 aacttatcgt acattggcgg ctaaaacgtc aaaaggagag atcagatgag tcaggtaagc
    36241 actgaattta tcccgacccg tattgctatt cttacggttt ctaatcgtcg cggtgaagaa
    36301 gacgatacct ccggtcacta tctgcgcgat tcggcgcaag aagcgggcca tcacgttgtc
    36361 gataaagcca ttgtgaaaga aaaccgctac gctattcgcg ctcaggtatc tgcgtggatc
    36421 gccagcgacg atgtacaagt ggtattgatt acgggtggta ctggcctgac ggaaggtgat
    36481 caggctcccg aagcattgct gccgttgttc gaccgtgaag ttgaaggttt tggtgaagtg
    36541 ttccggatgt tgtcgtttga agagattggc acttccacgt tgcaatctcg tgcggtagcg
    36601 ggcgtcgcca acaaaacgct gattttcgcc atgccgggtt cgaccaaagc gtgccgtacc
    36661 gcatgggaaa atatcatcgc gccgcagctg gatgcccgta cgcgtccgtg taatttccat
    36721 ccacatttga agaaataagt atgtcgcaac tgacccatat caacgccgct ggcgaagcgc
    36781 acatggtgga tgtctccgcc aaagcggaaa ccgtgcgtga agcgcgggcg gaagcctttg
    36841 tcaccatgcg cagcgagacg ctggcgatga ttattgatgg tcgccaccac aaaggcgacg
    36901 tatttgccac tgcgcgtatt gccggtattc aggcggcaaa acgcacctgg gatctgatcc
    36961 cgctctgtca tccgctgatg ctcagcaaag ttgaagtcaa tttacaggcc gagccggagc
    37021 acaatcgggt gcgtatagaa accttatgcc gcctgaccgg gaaaaccggt gtcgaaatgg
    37081 aagcattaac cgcggcctcc gtggcggcgc tgaccattta tgacatgtgc aaagcggtgc
    37141 aaaaagatat ggtgattggt ccggtacgtt tgctggcgaa gagcggcggc aagtcgggtg
    37201 actttaaggt ggaagcggat gattaaagtt ctttttttcg cccaggtgcg cgagttggtg
    37261 ggaacagatg caaccgaagt ggctgcggat ttcccaactg ttgaagcgtt acgccagcac
    37321 atggctgcgc agagcgatag ctgggcgctg gcgctggaag atggcaaatt actggctgcc
    37381 gtcaaccaga cgctggtgag ttttgaccat ccgctgactg acggcgacga agtagctttc
    37441 ttcccgccgg taaccggagg ttaagatggc agaaaccaaa attgttgttg gtccgcagcc
    37501 gttcagcgta ggagaagagt acccgtggct ggcggagcgt gacgaagacg gtgcggtagt
    37561 cacctttact ggtaaggtgc gcaaccataa cctgggcgac agcgtcaacg cattaaccct
    37621 cgaacactat ccggggatga ctgaaaaagc actggcagaa attgttgatg aagcgcgtaa
    37681 ccgctggccg ctggggcgcg tcactgtgat tcaccgcatc ggggaattat ggccgggcga
    37741 tgaaatcgtt tttgtcggtg tcaccagtgc gcatcgcagc agtgcgtttg aagccgggca
    37801 gtttattatg gattatctca aaacccgcgc accgttctgg aagcgcgaag ccacgccgga
    37861 aggcgaccgc tgggttgaag ctcgggagag cgatcagcag gcggcaaaac gctggtagtt
    37921 ttttgttagc cggataaggc accgctgcgt ccggcaattt caccgctcag tacatacgtt
    37981 tacgctgcgt cgatgcacag cctcatcatt ttgcagtatc cttaagatat tccttatatc
    38041 ttcaggagat cgtcatggac agattcccac gttctgattc aatcgtacaa ccccgggctg
    38101 gcttgcaaac ttatatggct caagtctatg gctggatgac cgttggcttg ttgctgaccg
    38161 catttgttgc ctggtatgcg gctaattccg cggccgtgat ggagctgttg ttcactaacc
    38221 gtgtcttttt aatcggtctg atcatcgcgc aattagcatt ggttattgtg ttatcagcga
    38281 tgattcaaaa gctgagcgca ggtgtaacga cgatgctctt tatgctttat tcggcgctga
    38341 cgggtcttac gctttccagt atattcattg tctataccgc tgcttctatc gccagtactt
    38401 tcgtcgttac tgccgggatg ttcggcgcaa tgagcctgta cggttacacc acgaagcgcg
    38461 atttaagtgg ctttggcaat atgctgttta tggcgttaat cggcattgtg ctggcatcgc
    38521 tggtcaactt ctggttgaaa agcgaagcat tgatgtgggc agttacctac atcggcgtga
    38581 ttgtctttgt cggattgacg gcgtatgaca cgcagaaact gaaaaatatg ggtgagcaga
    38641 ttgatacccg cgacacgtcg aacctgcgca aatattccat tcttggcgcg ttaaccttgt
    38701 atctggactt catcaacctg ttcctgatgt tgttgcggat cttcggcaac cgccgttaat
    38761 tctttttcac catcactatc ccggccatgt gtcgggattt ttttcttact aaaaacgaca
    38821 cttaacaagt accaggtata aaatatgcct tatggacgag cggaagcgtt ttctgtatcc
    38881 ataaatgcaa aagtattttg tatgcgtggt tttaaattta ttgatggtga atattaatat
    38941 tttttctaag gataactaaa agaatggaat catactcgca aaacagcaat aaattagatt
    39001 ttcagcacga ggccaggata ttaaacggta tatggctcat taccgcttta ggtttggtgg
    39061 caaccgcagg actagcctgg ggagctaagt atctcgaaat tacggcaacc aaatatgatt
    39121 caccaccaat gtatgtcgcc atagggttat tattactttg tatgtatggc ttaagtaagg
    39181 atatcaacaa gataaatgcc gccatcgcgg gcgtaatata tctgttttta ctctctttgg
    39241 tggcgattgt cgttgcaagt ttagttcctg tatatgccat tatcatcgtg ttcagcactg
    39301 cgggcgcgat gtttttaatc agtatgctgg ccggtttatt atttaatgtt gatcctggtt
    39361 ctcaccgttt tatcattatg atgacgttga cagggttggc cctggtaatc atcgtgaatg
    39421 cggcattaat gagtgaacgg cccatttggg taataagttg cttaatgatt gtgttatggt
    39481 caggcattat ctcgcatgga cgaaataagc tccttgaatt ggcggggaaa tgccatagtg
    39541 aagagttgtg gagtccggtt cgttgcgctt ttacaggtgc attaacactc tattactatt
    39601 ttatcggctt ctttgggata cttgccgcga tagctataac gcttgtctgg caaaggcata
    39661 cgcgtttttt tcattagccg taaatgcaca tactaaagaa cttaactaaa cttcacatcg
    39721 ccgcttcatt tttcgcccgt agcttcttcg cctggctttc cagcaacagg taacagatca
    39781 gcgccagcag cagtgggata aagtaataca gcacacggta agcgagtagg gcggcgataa
    39841 ttgtaccttt ggaggtatgc tccccagcca gtagtgcgat aaacaccgct tccagcacac
    39901 cgatccccgc cggaatatgc acgatgacgc cagcaatact actaaccagt aacacgccca
    39961 gtacaaagaa atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca
    40021 tccagttaac gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca
    40081 gcttttgccc tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa
    40141 ccgcgataat catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc
    40201 agtgatccgg caactccacc acgcctgcgg taaagataat ccctgccagt aaaatgtagc
    40261 ccagccagtt ggtggtaata ctgagcgaga aaatccgcgt aatagtgcta ccgggcaacc
    40321 ccagacgaga gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca
    40381 ggttgaaggc gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac
    40441 cgcagtaaaa gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca
    40501 gcccgaccgc actaagcagc gcaacgcgat tgtagtcgcg gatgaccttc cagacctctt
    40561 cccagtccac ttttttggcg tagaccacca gcaacacaat caccgcgata aaaaacagcc
    40621 aggtgaggat cttctttgct aagcgccagc gcgggtgtga tttactcatc agggttttac
    40681 ccccgtgttt tcagtttcta cccgatcctg cgtttccatt gtcggttgtg cgggcggatc
    40741 aacctgcgcc agacgtggcg tgtgtgccgg aagccagcca accagcgccg ggaagtggcg
    40801 taaaaagtgg aacgccagta cgcttttggt caggttccac caggtgcgtt tgggcagcat
    40861 ggtttcatcc acctgctgac aatctgcggc aataatgccg ttcagattat cgcgcagcgt
    40921 ctggttaaaa tgacgatcgt ggatgatgac atttgcttcg agattcagtg acaaactgag
    40981 cggatcgaga ttactggacc ctactgtcgc ccagtgatcg tccatcaatg ccactttgcc
    41041 gtggagcggg cggcggcggt attcaaaaac ctgaacgccg cctttaacca gatagttata
    41101 cagcaagcgc gcaccgactc tgacaatcgg catatccggt tcgccctgaa tgatcagttt
    41161 gatccgcacc ccgcgccgtg ccgctttacg caaggcgtgt aaaaatcgat agccggggaa
    41221 gaagtaggcg ttggcgataa tcacttcccg ccgcgcctga gtgagcattt tcaaataatg
    41281 gcgttcaata tcatcgcgat gttcttcgtt atcgcgccag accagcaata cctgcgcttc
    41341 tcccggctgg cggttctctt ccgctttgtg atgacgtcgc caccagcgtc gtgccgcgct
    41401 ctgtccaggc aggttttcca gctcaaactg gagaatatct tcgacaatcg gcccttcaag
    41461 gcgtaccgcg taatcctgtt tagcctctgg accgtagctg gacatatgct cggcggagta
    41521 attcagcccg ccaataaagg ctatacgcgc gtcgatcacc acaattttgc gatgcatccg
    41581 gcgaaacaca ttggtgcgca taccaaaaag gcgagggcgg ggatcgtagt agcggaacac
    41641 tacgccagct gccgtcagtt cattgacaaa ctcatcgctg agatccggcg aaccgtagcc
    41701 atccagcaag acttccgctt taaccccgcg ttgcgctgct gccagtagtg ccgcatgcag
    41761 ttgtttgccg acgtcatcct caaaccagat aaacgtttca agaatgatgc gttcttgtgc
    41821 ctcgccaatc gccttaaaca ccgcgggata atattgctcg ccgttttcca gcaactggat
    41881 cttattgcct tcgcgccagc tacatttcat aaatgaatct ccgcacttaa aggggcatga
    41941 tcagaaaggt gtcgccatgt ccgcagcggc aacgcggttg gcgcgctggc gctggcattt
    42001 ttgacgtaga tcctgtccag tcgtagtaga ggaaattgca ccggaaacgt gcgcgccggg
    42061 cgtccgtggg cgcgggtaaa aatctcatcc agtccggcct gcacttttaa cggatgatta
    42121 gctttttgcc gccagtcatt gaaatcaccc gccaccaata ccggttcgcc gtccggtagc
    42181 tcattcaccc attcggcgag catcgcaagc tgcgcctgac ggtgcgcctc acgcaggccc
    42241 agatgtacgc acatcacatg aatcgctttt ccggtcatcg gcggcacaat gcggcagtag
    42301 agcacgccgc gcttttccgc accatcgacc gaaacatcgc gattctcata atgttcaatg
    42361 ggataacgcg acagtacggc gttgccgtga tgcccttccg ggtatacggc attgcgaccg
    42421 taggcaaaat cgctccacat agtgtcggcg agaaactcgt agtgcgaggt atcgggccag
    42481 ttttccacat gcagcggatg aacttcgtgc gcgcccatca cttcctgcag gcaaacaata
    42541 tcggcgctga cggtacgcac ggcgtcgcga agttccggca aaatgaagcg tcggttaaac
    42601 gcggtaaagc ctttgtgaat attgatggtg agcaccttga acgaaaattg ttgtgtttga
    42661 tcgggcataa ttttcctgtc tttgcctctt atctcattga aatagtgtag tcggcgtcac
    42721 aaaaaggtgc ggtcttacgg aattttccgt aaagttcggt actctgagta agtagagata
    42781 aattcttcag gagagaagcc atgaagtggc aacaacgtgt tcgtgtcgca acgggtctaa
    42841 gttgctggca gattatgttg catttactgg tagtggcgct gctggtggtg ggctggatga
    42901 gtaagactct ggttcacgtc ggcgtgggat tatgcgcact gtattgtgtc acggtagtga
    42961 tgatgctggt gtttcagcgc caccccgagc aacgctggcg tgaggtggca gacgtgctgg
    43021 aagagctgac cacgacctgg tattttggcg cagcgctgat tgtgctgtgg ctgttgtccc
    43081 gcgttctgga aaacaacttt ttgctggcaa ttgcagggct ggcaatcctt gccggcccgg
    43141 cggtagtgtc tttgctggcg aaagataaga agttacatca ccttacgtct aaacatcgcg
    43201 tacgccgctg accctgtcgt ggccgttatc accagtagcg gccacaaact attccacaca
    43261 atatccagac tcgcatcctt caaataaatc tgcttggtaa tgtccgtaaa gtggcgaata
    43321 gggttaatcc acgtcaggtt ttgcagccat accggcatgt tttccaccgg agaaacgtaa
    43381 ccggaaagga gaatggcggg catcataaag acaaacacgc cgataaacgc ctgctgttgt
    43441 gttgaacaga gtgatgaaat caacagaccg aatcccacca gcgataaacc ataaatcacc
    43501 atcgtaaagt agaacagcgc cagcgatccg gcgaagggga tttgatacgc ccagatacca
    43561 atcgccagca caatggtggc ctggaaggtg gcgacaatta acgccggtac ggctttgccg
    43621 atgaagatct gccaggtggt gagcggcgaa accagtagct gatcgagcgt accttgttca
    43681 cgttcgcggg cgacggaaag tgaagtgacg atcattacgc cgatagtggt gatcatggcg
    43741 atcagtgacg gcaccacaaa ccatttgtag tcgagattcg ggttatacca gttgcgtacc
    43801 accagctcgc tgttgttagg tttcggtttt ccttccagca gctcctgctg ataatttttg
    43861 acgatctgtt gcaggtagtt ggcggcaatt tgcgcactgt tggagttacg cccgtcgagg
    43921 atcaactgca aaggcgcggt ctggaaggta tccagtttgc gcgagaagtc agccgggaaa
    43981 cgcaccagta gtaacgcctt ttgtgtgtcg atggttgggc ggatctcctg tgggcttttc
    44041 agcagcagca catgagtaaa ggcgctggcg cgggcaaaac gttgggtcag ctccaccgaa
    44101 tgctcgccgt tatcttcatc gtagatggcg atggtggcgt tagtcacttc cagcgtggcg
    44161 gcgaacggga acaggatcac ctgaattagc acgggtaaaa tcagaatcgc gcgggtttgc
    44221 ggttcgcgca gcaacgactg caactctttg cggattaacg tccataagcg atgaaacatg
    44281 ctcttctccc taatccagcc gacgtttggt tttcagccac gtcaggccga taaacatcac
    44341 cgccgaagcg atcaaaaaca gcacgtttac caccagcacc actggaatat tcccggcgag
    44401 gaacaggctt tgcagggtgc tgacgaaata acgagcggga ataatgtacg tcaccgcgcg
    44461 gatcaccgcg ggcatactgt cgatctgaaa aataaagccg gaaagcataa tcgacggcag
    44521 aaaagcggcg tttagggcga cctgagcggc attgaactgg ttgcgggtaa tcgtggaaat
    44581 cagcagcccc atccccaggg tactgagtaa aaacaggctg gagataaaaa acagaatcag
    44641 cagcgacccg cgatacggca cgccgagaat aaacactgac accagcatac acagcaacat
    44701 cgccagcatc ccgagaaagt aataagggat cagcttacac agcagcagtt ccgtgcgggt
    44761 aatctccgta gagagcagag cctccatggt gccgcgttcc cattctcgcg ccaccaccag
    44821 cgaggtgaga atcgcgccga tgaccgtcat gataatggtc accgcaccgg ggataatgaa
    44881 gtgctggcta atcgccgccg ggttaaacca gtagcgggtt tgtacatcaa taagcggttc
    44941 aaaagtctgc ccgttgtcct ccgctcgctg catttgccag atctgccaga tcccttcgac
    45001 atacccctgt acaaagttag cggtattcgg ctcactgccg tcggtgatca cctgaatcgg
    45061 tgcggtggcg ttggcgcgct ccatctgttc cgcaaaatcc accggaataa ccaccagacc
    45121 gcgaattttc cccgcctgca ttttggcgat cagttcctga cggttatcgc tgatggtggc
    45181 gtcgatgtag ggcgaaccgg tcatggtgtg ggtgaaatcc agcgccgctt cgctacgctg
    45241 ttccagtaaa atcccgaccc gcagcttgct ggagtcgagg ttaatgccgt aaccaaaaat
    45301 aaacagcagt agcagcggga tcactaccgc aatcagccag ctactcggat cgcgaacgat
    45361 ctgccgcgtc tctttaacgc acagcgcccg tacgcgacgc caggacagga tcgggttact
    45421 cattgctatg ctccttatcc cagtcgtgga tcaactgaat aaaggcttgc tccatggtgg
    45481 gatcgggttg ctcatcgtta gccgactgtg ctttcaaatc gtccggcgtg ccgctggcga
    45541 ttaatttccc gcggtacacc aggccgatgc ggtcgcaata ttccgcttca tccataaagt
    45601 gggtggtgac catcaccgtg acgcctttct ctaccatgct gttgatgtgc agccaaaatt
    45661 cacggcgggt gagggggtca acgccggaag tcggttcgtc gagaaacaga atgtccggtt
    45721 catgcatcag cgaacaggcc agcgccagcc gctgtttaaa acctaatggc agttcatcgg
    45781 tggcgtggga ggcgatactt ttcaggccga acgcctcgct catgcgggag attttttcgt
    45841 tctgcgcccg accgcgtaag ccatacacac cagagaaaaa gcgtaaattc tgttcgaccg
    45901 tcaggttacc gtagagcgaa aatttttgcg ccatatagcc gagatgctgg cgcgctttac
    45961 cggaactctc tttcagatcc atccccagca ccagcgcctg gccggaagtc ggcaccagca
    46021 aaccgcacat catcttaaag gtggtcgatt tacccgcgcc gtttggcccc agcaaaccaa
    46081 aaatctcccc acgtttaacg gcaaagttga cgtgatcggt ggcggcaaaa tccccaaatt
    46141 tcttggtcag ttctttcgct tcgatcaccg tctcgccggg tgtgccttct accgtatgta
    46201 atattgcgcc cagcggcgat tccgaggttc cggcaccgcc cagcaaatca ataaacgcat
    46261 cttcaaaacg cggcgtagtt tcgttgatgt tgatttccgg catcccgtcg gcatggcgaa
    46321 tatcgtctgg tgtggcctct tttttgagga tcagacgtac cgatttcccc tgaatcatgc
    46381 cgtcgctgac ctgcggcagt ttcaaggcgc gttgcaacag tttgcggttg ccctcgtgtg
    46441 gactggtcat cagaaagctg cgtccggcca tggtttgtgt cagggctttt ggttctccct
    46501 gatacagcaa ctcgccttcg ttcatcagta acacgtcacg gcactgctcg gcttcgtcga
    46561 gatacgaggt actccagagg attaacatcc cttcgcccgc cagctcatgc accatctgcc
    46621 acagttcgcg ccgtgagata gggtcaacgc cgacgccggg ttcatcgagc agcaacactt
    46681 tcggttcgcc caccagggta caggccaggc cgagtttttg tttcatccca ccggagagct
    46741 tgcccgccag gcgtccggta aacggcccaa gagacgtaaa ctccagcagg cgagcaaaag
    46801 tttgcttacg tgcctcgccg gtgacgctgc gcaaatccgc gtacagattg aggttctcca
    46861 tcaccgtgag atcttcatac agaccaaatt tctgcggcat ataaccgagc acggcgtgca
    46921 gcgcgccgtc gtttttgatc ggatcaaagc caatcaccgt ggcactgccg ctgtcgggtt
    46981 tcagtaatcc cgccaacatc cgcatcagcg tggttttacc tgcaccgtcc ggccccacca
    47041 accccgtcac ataaccggcg tgaatggtac aatcgagcgg cgcgacggcg ggcttgtcca
    47101 tgcccggaaa gcgtttttcc aggccgttca gcgtgataac ggcatcattc atgtcctgcc
    47161 tcgtcaccga attgtaccgt cactggcatt ccctggcgta acgcatcatc ggcgtcggtc
    47221 accacaatac gcaggcgata gacgaggtcg gtacgcagat ccggcgtttc gacggttttc
    47281 ggggtaaatt cagcagtcgg cgaaacgaaa ccaatctgcc cgtgatacgg cttgtccggg
    47341 cgaccatcgg tataaagcag cactttgcgc cccggctggg cctggtcaag attacgttca
    47401 tcaacataag cgcgcaccca caccggacgc gttagtgaaa cggtaaacac cgtgccacct
    47461 tcattgagga ccgtgcctgg ctccaccgcg cgcgttaaca gcgtgccatc agacggggct
    47521 atcaacgttg agtcctgtaa attcaactcc gcctgcgcca gttgcgcctg cgcctgttcg
    47581 aggctggctt tcgcctgagc gatgtcctgt tcacggttac cggaacggta ctgacgcaat
    47641 ttatcctgtg ctgatttcag cgttgcctgc gcctggtcgc gcgaggagcg ggcattttcc
    47701 aggtcatttg ccgaaatagt gcggcttttc cacaaccctt gctggcggtt atagaagttc
    47761 tgcgcatagt cataggcggc ttgcgcctgt ttcaccgctg cggcggcctg agcgatttct
    47821 tcatcgcgat acccggcaag catcaggtca tactgcgcct gtgccaccga aacacccgct
    47881 ttcgcctgca tcagggcaat ctcatacggc ttgtgatcca gttcgcccag cacctggccc
    47941 gctttgatag catcaccttc gtccaccgcc agcgattcaa cgcgcccccc aacacggaaa
    48001 ctaagattta ccgtacgaat atccacgttg ccatacagcg tcaggccgtt atcctggcgg
    48061 ctttgatacc accagtagcc tccggcaacc acggcggcca gtaccactac cgccagtccg
    48121 atcacgacag gttttttcat cactacaaac tcctttgcga taatccttgc aaaatcaggt
    48181 cgatatgaca ggtcaccgtc tggttgatca gttcggtctt ttcttcatcg aacgcggtcc
    48241 agccggtacg taacagaatt gtttctttgc caagacggaa cgccagaatc tcaccaatca
    48301 acgcatgggt atgaaggatc atgcgggtgt cattggcgtc gcagccggtc caggcggcaa
    48361 tcaggcgtgt caggtggctg tgtagcggac taatcacctg ttcgtgcacc aggtggtagg
    48421 ctgccgtggg agagagctgc tcacgggaga taaacttgct gaggttgacg gtgtcatcct
    48481 gggtgagcag cttaatcatg ttcctgcagg cgcgaaggat cagttcacgg atggcagccc
    48541 gatcaggctg tggttgcgcg aacaagcgtt cggcttcctc ggcatgcgga cggaactgct
    48601 cgccaataaa atcggcaatc cactgggcgc aggcgaggta caaatcttct ttcgaaccga
    48661 agtagtaggt gatggcagcg atattctgcc cggcctgggc ggctatctcg cgagtggtgg
    48721 cgttcattcc atattcacca aactgcgcca gtgcggcagc aatcagctgt tttttcgcct
    48781 gttcaccctt gattgtcatg gcaggattat tcatcgcaca gtccattctt aatcaaatga
    48841 ttgattaaga ttatgactcc ataggggagt tgtccagtat ggctaagaat tttagcaacg
    48901 ccagtcacag ggataattta tgcgctgcgt cacaaaaact gctacactcc gctccctcat
    48961 gacattgtgg tttttgtcat tttccttttc agtatctccc tgaaaactac accggtaacg
    49021 gtcggggcgg ttcggagtag ttatgtcttt cgattctttg ggtttaagcc ctgatatcct
    49081 gcgcgccgtt gccgagcagg gttaccgtga acccacccct attcagcagc aggcgatccc
    49141 tgcggtgctg gaaggccgcg acctgatggc tagcgcccag accggcaccg gcaaaacagc
    49201 gggctttacg ctgccgctgt tgcaacacct gatcactcgc cagccgcacg ccaaagggcg
    49261 tcgtccggta cgtgcgctca ttcttacccc gacccgtgaa ctggcggcgc agattggcga
    49321 aaacgtccgt gattacagca aatacctgaa cattcgttcg ctggtggtgt ttggtggtgt
    49381 gagtattaac ccgcagatga tgaaactgcg tggcggcgtt gatgtgctgg tggcaacccc
    49441 gggacgtttg ctggacctgg aacatcagaa tgcagtgaag ctggatcagg ttgaaatcct
    49501 cgtcctcgat gaagctgacc gcatgctcga catgggcttt atccacgata tccgtcgcgt
    49561 gttaacaaaa ctacctgcga agcgccagaa cctgttattc tccgcgacct tctctgacga
    49621 tattaaagcc ctggcggaaa aactgttgca caacccgctg gaaatcgaag tggcacgccg
    49681 caataccgcg tctgatcagg tgacccagca cgttcacttt gtcgataaga aacgcaaacg
    49741 cgaattgctg tcgcacatga ttgggaaagg gaactggcag caggtgctgg tgtttacccg
    49801 taccaaacac ggcgctaacc atctggctga acagctcaat aaagatggca tccgtagtgc
    49861 ggcgatccac ggcaataaat cgcaaggtgc gcgtactcgt gcgctggctg attttaaatc
    49921 gggcgatatt cgtgtactgg tggcaactga catcgctgcg cgcggcctgg atattgaaga
    49981 gctgccgcac gtcgtcaact atgaactgcc aaacgtacct gaagattatg tccaccgtat
    50041 cgggcgtacc ggtcgtgcgg ctgctaccgg tgaagcgttg tcgctggtgt gtgttgatga
    50101 acacaaactg ctgcgtgata tcgaaaaact gctgaaaaaa gagatcccgc gcattgcgat
    50161 tccgggctat gagccggacc cgtcaatcaa agccgaaccg atccagaacg gtcgccagca
    50221 acgtggcggc ggcggtcgtg ggcaaggtgg tggtcgcggt caacagcaac cacgccgtgg
    50281 ggaaggtggc gcaaaatctg caagcgcgaa acctgcagaa aaaccgtctc gccgcctcgg
    50341 cgatgccaaa ccggcaggcg aacaacaacg tcgccgccgt ccgcgtaaac ctgccgctgc
    50401 gcagtaatct tttatgccgg gctatgcccg gcatcaggct gatgaacaaa cgcaaaactg
    50461 cctgatgcgc tacgcttatc aggcctacgt gaactctgca atatattgaa tttgcatgct
    50521 tttgtaggcc ggataaggcg tttacgccgc atccggcatt tcacaacaag cacttgtcag
    50581 caatttgaga acacgggaaa aaatttatct gttttaccgc ccataagcca ccgagacgtt
    50641 acttctctat agccaattgt tcgcgcaact ccattaaaag gtagcccagt cgattcttgc
    50701 ccttaccatg accaccgtct ccccagtaag catcgttttc cgtatgctca accagttttg
    50761 cgggcgcggt tgccagcaag agcgcacgca gttctgcatg ctgttcgaat ttagcgcgaa
    50821 gtgcttttcg catcacctgt tctttgaccg actcccagtt tttacgcaga ggcttagaac
    50881 gatcgcgccc catgcgtgcg gcgaccatag gggaagaaac ccgacggatc tcttcgcggt
    50941 atttttcatc aaggaatttt tgtgcctgaa aatagtgttc tgaggtgggc caggttttcc
    51001 cgtcaacctt gatgggccag gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc
    51061 tgtagaaatt tatgatggtg tcttgcatga cgtgttggat cctttgtgct cgaacgggca
    51121 ttaaaccgca ttatgttggt ggttattgcg agccgctttc cagaaacaga aaaaccatta
    51181 cccctgaaaa ccgaaaaatg ccacaatatt ggctgtttat acagtatttc aggttttctc
    51241 atggcattaa ccgccgcgct taaagcgcaa attgccgcct ggtataaggc gcttcaggaa
    51301 cagatccccg actttattcc ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa
    51361 acgctggccg gagaagaagg gcgacatctg gcgattgaag cccccaccgg cgttgggaaa
    51421 acgctctctt atttgattcc cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg
    51481 gtgagtaccg ccaacgtggc attgcaggat cagatttaca gcaaagattt accgctgctg
    51541 aaaaagatca ttcccgatct taaattcact gccgcttttg ggcgtgggcg ctacgtttgt
    51601 ccgcgtaatc tgacggcgct cgccagtact gaacccacgc aacaggatct gctggcgttt
    51661 cttgacgacg aactgacgcc gaacaatcag gaagagcaaa aacgttgtgc gaagctgaag
    51721 ggcgatctcg atacttataa atgggatggt ctgcgcgatc atactgatat cgctatagat
    51781 gacgatctct ggcgtcgttt aagtaccgac aaagccagct gcctcaaccg caattgttac
    51841 tactatcgtg aatgcccgtt ttttgtcgct cgtcgggaga ttcaggaagc ggaagtggtg
    51901 gtggcaaacc atgcgctggt gatggcggcg atggaaagcg aagcggtatt gcctgacccg
    51961 aaaaatttac tgctggtgct ggacgaaggc catcacctgc cggatgtggc gcgggatgcg
    52021 cttgagatga gcgccgaaat caccgcgccg tggtatcggc tacagctgga cttgttcacg
    52081 aaactggtcg ctacctgcat ggagcagttt cgcccgaaga ccatcccacc gctggcgatc
    52141 cctgaacgtt tgaatgcgca ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac
    52201 attctcaatc tctacatgcc tgccgggcag gaggcagagc accgttttgc gatgggcgaa
    52261 ctgccagatg aagtgctgga gatctgccag cggctggcaa aactcaccga gatgctgcgt
    52321 ggcctggcgg agttatttct taacgattta agtgagaaaa ccggcagcca tgacattgta
    52381 cgtctgcatc ggttgatttt gcagatgaac cgcgcgttgg ggatgttcga ggcgcaaagc
    52441 aaactctggc ggctggcttc gctggcgcaa tcttccggtg caccggtgac caaatgggcg
    52501 acgcgggaag agcgcgaagg gcagctacac ctctggtttc actgcgtggg aatacgtgtc
    52561 agcgatcagc tggaaaggct gctgtggcgc agtattccgc acattattgt cacctccgca
    52621 accttgcgtt cgctgaacag tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa
    52681 gcgggcgacc gttttgtggc gctggattcc ccctttaacc actgcgaaca gggcaaaatt
    52741 gttattcccc ggatgcgcgt tgagccttcc atcgacaacg aagagcagca tattgccgaa
    52801 atggcggcct ttttccgtaa gcaggtggag agcaaaaaac atctcggtat gttggtactg
    52861 tttgccagcg gacgggcgat gcagcgcttt ctcgactatg tgacggattt acgtctgatg
    52921 ttgctggttc agggcgatca gccgcgttac cgtttagttg aactgcaccg caaacgcgtc
    52981 gccaacggtg agcgcagcgt gctggtgggc ttacagtcat ttgccgaagg gcttgatttg
    53041 aaaggtgatc tgctcagcca ggtgcatatc cacaaaatcg cttttccgcc catcgacagc
    53101 ccggtggtga tcaccgaagg ggaatggctg aaaagcctca accgctatcc gtttgaggtg
    53161 caaagcctgc cgagcgcctc gtttaacctg attcagcagg ttgggcgact gattcgaagc
    53221 cacggttgct ggggcgaagt ggttatctac gacaaacgct tgctgaccaa aaattatggc
    53281 aagcgactac tggatgcatt accggtattt ccgatagagc aaccggaagt ccctgaaggt
    53341 atagttaaaa agaaagaaaa aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag
    53401 tcaggtaagg agtcgtaaat ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa
    53461 aaccacgcgc gcgatttaga ccgggatact gcgcgcggtc tgtatgctca tatgctcaat
    53521 ggtgaagtcc ctgaccttga gttgggcggc gtattgattg cgctgcgtat caaaggagaa
    53581 ggggaagcag agatgctcgg cttttacgaa gccatgcaaa atcacaccat caagctgacg
    53641 ccgccagcag gcaagccgat gccgattgtc atccccagtt acaacggcgc gcgtaaacag
    53701 gccaacctga cgccgttgtt ggcgattctc ctgcacaaac tcggttttcc tgtggtggtt
    53761 cacggggtta gcgaagatcc aacccgcgtg ctgactgaaa ccatttttga attgatgggt
    53821 attacgccaa cgttgcacgg cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg
    53881 tttatgccag tcggcgcgtt ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg
    53941 atgggcgtgc gtaacagtgc acataccctg gcgaaactgg cgacgccatt tgccgaaggt
    54001 gaggcgctgc gtctttccag cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc
    54061 tttagcgata tcggcgggcg ggcgctacta atgcatggta ctgaaggtga agtgtatgct
    54121 aatccgcagc gctgcccgca gatcaatctc attgaccgtg aagggatgcg ggtgctgtat
    54181 gaaaaacagg acactgctgg tagcgagtta ctgccacaag caaaagatcc ggaaaccacg
    54241 gcgcagtgga ttgagcgttg ccttgctggc agcgaaccga ttcccgaatc gctgaaaatc
    54301 cagatggctt gctgcctggt ggctacgggt gaagcggcaa ctatcagcga cggcctggcg
    54361 cgcgttaatc aggcatttta attcttttct cagccggatg agccatgtca tccggctttt
    54421 accccaccgc aatatgaaat tcctgcatct ttattgacct tcccacgccc ggcgtgcagc
    54481 ataaaaatac aacaaacaca taacataaac aggagttaac catggaaagt ggtcatcgct
    54541 ttgatgctca gacgctgcac agttttattc aggctgtatt tcgtcagatg ggtagcgagg
    54601 aacaagaagc gaaattagtt gccgatcatt taatcgcggc aaacctggca gggcatgatt
    54661 cacatggtat tggcatgatc ccaagctatg tacgctcctg gagtcagggg cacctgcaaa
    54721 ttaaccatca tgccaaaacc gttaaagagg cgggggcggc ggtcacgctc gatggcgatc
    54781 gcgcatttgg tcaggtcgcg gcacatgaag cgatggcgct ggggattgag aaagcgcatc
    54841 agcacggtat tgccgccgtg gcgctacata actcgcatca tatcggccgt atcggttact
    54901 gggcggagca gtgtgcagcg gcggggtttg tctctatcca ctttgttagc gtggtcggta
    54961 ttccaatggt cgcgccgttc cacggtcgcg acagccgctt tggcaccaat ccgttctgtg
    55021 tggttttccc tcgtaaagat aatttcccgc tgttgcttga ttacgccacc agcgccattg
    55081 catttggcaa aacccgcgtc gcctggcata aaggcgtccc cgtgccgcca ggttgcctga
    55141 ttgacgttaa cggcgtgccg acgaccaatc cggcggtaat gcaggagtcg ccgttgggtt
    55201 cgctgttgac ctttgccgaa cataaaggct acgcccttgc agcgatgtgt gaaattcttg
    55261 gcggggcgct ttccggcggt aaaacgacgc atcaggaaac gttacaaacc agtcccgatg
    55321 ccattcttaa ctgcatgacc actatcatca tcaacccgga actcttcggc gcgccggatt
    55381 gtaacgcgca gaccgaagcc tttgccgagt gggtgaaagc ctcgccgcat gatgatgata
    55441 agccgatttt gctaccgggc gagtgggaag tgaacacgcg tcgcgaacgg cagaagcagg
    55501 ggattccact ggatgcggga agctggcagg ccatttgtga tgcagcgcgg cagattggta
    55561 tgccggaaga gacgttgcag gctttctgtc agcagttagc cagctaaaaa aaagcccgtc
    55621 cagtggcgga cgggcaaaca agggtaacat aggatcaatg agggttagag catatgcgtc
    55681 tgtcggcaaa cagacaggga aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc
    55741 aacggttcgg attttgtagg cctgataaga cgcggtagcg tcgcatcagg catctgttgc
    55801 cggatgcggc gtaaacgcct tatccgtcct acgaattact tataaattac cgcagtaccg
    55861 cttaatttat tgttgttggt ggcggaagtg atgctgtatc cactggcacc ggctgccgcg
    55921 gctttctcag ccagtttcgc ttccagggca tcgagggtgg atgcgccatc ggcagaaacc
    55981 acgccgattt tattcatgtt ctgtgcctgg gatgccgtta ccggttccgc cgcgaacacg
    56041 ccaaatgaca gagttgaaag agccatagca gcaacaacag tattgatagt tttcataatt
    56101 aatctctcgc aggtgatttt tgtataagtg acgttgtttc gtcgatgtga tgagtatcac
    56161 gtttttttac gagagataaa atcgaagaga attgaccgcc ttgttcaaat aaattgattg
    56221 atatctaacc tactgataaa ccatttattt gttgtactta tgaacttgtt ttataccgcg
    56281 tggcagtcac agcagcgtta aaggcacgga atgacacgca aaatacaaat tatactcgct
    56341 atgcatctcg ccagttgtag ttaacgtaac tggctgtctt tcgaacctct gcgattatat
    56401 cctgtataag ctggtttttg taaatctttc tcctgctgac aatgaataca taagcgcacg
    56461 ccaggaatgg cttcccgacg ggcctgcggg atgggggcac cgcactcttc acattcatcc
    56521 aggctttcgc cgcgcggaat ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg
    56581 ttgatctgtt cgttgacggc gtcatcgtta gcccaactgg atgccatatc gacctcccca
    56641 tatcaatact tgtacagtta agtgtagcta atccagggac gaactcgggc agttcaagca
    56701 tcagatctcc gaccattccc gcagcagatt atgataaaga ttaagcagcg acaggatctc
    56761 ttcactttcg ccgtagcggc ttttcagcga ctgaatattg ttgtccagtt caaacagcat
    56821 ggcgcgcttt ttatcatcgc ggatcatcga ctggatccac ataaatgatg ccactcgtac
    56881 gccgcgggtt acgggtgtca cgcaatgcag gctgctggag ggatacaaca cgagatcgcc
    56941 tgccgggagt tttacccgat gttgtccgaa ggtgtcatta acgaccagtt cgccgccgtc
    57001 gtagctttgt ggatcgctta aaaacagcgt ggcagaaagg tcagtacgca tccagccgtt
    57061 ttgtggatgg ctgcgtactg cgccatccac atggaaacca taggtttcat tgttctgata
    57121 gcgattaaac agcggcgtgg aaagggtacg cggcaaggcc gcggcaaaga ataaagcatg
    57181 ttggttaacc gcgttcagca cctcattttg cagggcggcg tataacgtgc tgcgggtgtc
    57241 gacctgttga ttgttcttaa cttgcgcacc ttgtgcgccg gtggtgacgc gtccatccac
    57301 ccattcggct tgttccagtt gttcgcgaaa acgagcgacg tcctgtggcg ataacacgcc
    57361 gggaatgtgg tacatcatac aatttctcca aaaagtgggg cctgcgcccc acatctgaat
    57421 cagaaatgca tattggctgt gagcaagaag gttcttggct cgcccgggtg ataacggtag
    57481 ccgctcttgt tgattgaggc gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg
    57541 aagtcgagat tgcgattaac tcgatacccc agtttggcat cggcgaccca gtaaccttcg
    57601 gtaaacgctg gcgttcccac cgcgccgtct gaacctttat gcatactgcc gatatagcgt
    57661 gcgcccgcgc caacagagat atcgtcggtt gcctgatatt ggctccataa ggtgaaggcg
    57721 tgctccgggg tatacggcag cgatgaggaa ccatcctggg caacatcttt gccgtttttg
    57781 atggttgctt tttgctgggt atagccgcca atcacctgcc acgcgggagt gatattcccg
    57841 gccacggata tctcatagcc ttcgacgcgt ttcttaccgt attgcgagta agttccgtca
    57901 tcattttgct caacttcatt ttcgatatca gtgcggaaca gcgcggcggt gagcaacaga
    57961 cgtttatcca gaacctgcca tttggtgcca atctcgctgg tgttggcttt ttgcggttta
    58021 aaatcggtgc ggttggcact gttaccgctg ccagactgcg caagggcgaa gttgttgccg
    58081 cccggaggct gctgggaaac ggcatagtta atatagacat tgccgttttc cgtcaggtga
    58141 tacagcgccc cggctttcca gttcatcaga ttgcccgact tggcggtgtc gacggtggtg
    58201 accggagaac cttttgccac accagttggg caggtgatgg caccgcgtcc gctgccgccg
    58261 caggcggtgg cactgtcata ttcagtatga taattatcca gacggatccc gccgttcagc
    58321 tcaaaatcac gggtgatttg cagcgtatca aaggcgtaaa ttgcgaaggt atccgtctga
    58381 ccattggcgt ttgcgccgtt gcgcgtcagg ccgccgggat gaatgctgct gtcaggatga
    58441 taaatattta ccgcgggtaa cgtcaccgga ttaacgccgt agttagtctg cgtttcacgg
    58501 gtaaattcca cgccggtact gacatcatga ccgatagaac cggtatagaa cgtcgaggtc
    58561 aggttggtct ggttggtgag aattttatta ctcacatctt tggtattcgc cgtgcgtgac
    58621 caggtccagc tattcacatc gctggtgggc tgagtaatat tcgacgcccc gcccataatc
    58681 gccgtcatca ggtaatcctg ctttacgcgc gaccaacggg tagtattgcg aatggtggtg
    58741 ttatcgttga tgtcgtgctc aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa
    58801 tcggaatccg tgccgtaaaa gttatgagta tcaacttttc cggaatgatt cagggccgcc
    58861 gttcccgcag atggggcaga atagcccggc aaaccgatgg tcggaatgcc gccgtctggc
    58921 gtgttgtgct gggtgacatg cagataatta agatacaaac gattcgctgt accaaggcca
    58981 aaagcgacag aaggggcgac gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg
    59041 tgcgtttttt cgcccattac attcaggcgc accgcggtgg tatcaccaat gacctgattg
    59101 acgtccagcg tgccgcggcg gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg
    59161 gaatcattgc gcggctgctt gctgatcata ttgatcgagc ctgtcggtgc gctgcgcccg
    59221 tagtcggtgc cggacggccc tttaatcact tcgacctgct cggtattgaa ggtgtcgcgc
    59281 gagacgctgc cgatatcgcg aatgccatca atataaatac tgttagaggt atcggcacca
    59341 cgcatataaa tggcgtcgcc agtggtggag ttaccgttct cacccgcaaa aaacgcaccc
    59401 acgccgggga cgtttttgag cgcgtcggta aggttggttg cgccctgatc tttaatcact
    59461 tgttcagaaa ttaccgtcat cgtgcgggta gtatccgcta ccggacgcga gaatttcgga
    59521 tcggcagatt gttgtggcgc ataaagcgaa ggcgttgatg cttcgacaac cagcgtgtca
    59581 tccgcgttag tttgcccttc ggcggcgagt gcctgagcca caggcgtgat gccaatacaa
    59641 agaccggcaa agaacgtgag cgaatgaaat tgtctggcag ggaaattgcg attgttttcc
    59701 attttgcagg tgactttttc ttatatgttg aaattttcac tgccacgaga aagcctctct
    59761 gcggatgcga tgccactgtt aatcccgtga tagtgctcgt gaaagtgata ataattttga
    59821 taagcattat cacttggagc gattttctat cacccattgc gcgaaaaaga aatacattta
    59881 tttacattga gttcatattt gatgtttttt gattacaaaa cagacgaaaa ctgcatccag
    59941 aggcgaatgc agttacgttg gtggggttat ttgtagatgg ttgcagtacc gaacatctgg
    60001 tcatttccgc ctgcggaatt aatgacataa cctttcgcgc cttgttcgcg cgctttttct
    60061 gccagtttat actccagatc gcttaggttt gatgcgccag ttgccgaaac ggtaccggcg
    60121 ggccgtagct gaccgctgtc caggttactc atcggttgcg cggcataagc cgtgagagag
    60181 attccgctca ggacggtggc aatcagtagt gtgaggcact ttttcataat gacatcctca
    60241 taaatacaac gggtagtgcc tgataagtgt aggtcgcctg cgcgatgcaa atgacgcaaa
    60301 aaattgacag agatgtgctt attttttgaa cgatatattt ttacaaaata agacaaatca
    60361 atgacatgca taatgctgat ttttatgatg aattgccata aaccatagcc atgttaaggt
    60421 tttggggaat gataaacgca gccagatatt taccctgtcg cgttatgcgg gtatcttacg
    60481 ccgctgctta aaggagaatg ctatgtccgc ccagaaaccg gggttgcatc cgcgcaaccg
    60541 tcatcacagc cgctacgatc tcgccacgct ttgtcaggtc aatcctgaac tcaggcaatt
    60601 cctcacgctt acacccgccg gggagcaaag cgtagacttt gccaatccgc tggcggtgaa
    60661 ggcgctcaat aaggcgttgc tggcccattt ttacgccgta gcgaactggg atatccccga
    60721 cggttttctc tgcccaccag taccgggccg ggcggattat attcatcacc ttgccgattt
    60781 actggcagaa gcaagcggaa caattccagc gaatgccagc attctggata tcggcgttgg
    60841 tgcgaactgt atttatccgc tgattggcgt acatgaatat ggctggcgtt ttaccggtag
    60901 tgaaaccagc agccaggcgt taagcagtgc gcaggcgatt atcagttcta atccggggct
    60961 taaccgcgcc attcgtctgc gtcggcaaaa agagagtggg gcgattttta acggcatcat
    61021 ccataaaaac gagcaatacg acgcgacctt gtgtaacccg ccattccacg attccgccgc
    61081 tgcggcacgg gcaggtagtg agcgtaaacg ccgtaacctg gggctgaaca aagacgatgc
    61141 actgaacttt ggcggccagc aacaggagtt gtggtgtgaa ggcggtgaag tcacctttat
    61201 caaaaagatg attgaagaga gcaaaggctt cgcgaagcag gtgatgtggt ttacatcact
    61261 ggtatctcgt ggtgaaaact taccgccgtt gtatcgtgcc ctgacggacg tgggcgcggt
    61321 gaaggtggtt aaaaaagaga tggcccaggg gcaaaagcag agtcgcttta ttgcctggac
    61381 ctttatgaac gacgagcagc gccgccgttt tgtcaatcgc cagcgttaaa gcgttggttc
    61441 ccccggcggt aacggttcag ccggggtcgc gcccggagca ggcagcacct gataagtctg
    61501 cactggcgcg cgaacgcccg ccaggtcgaa atgttttttc acctggctgt cgagggcaaa
    61561 gcgtaccgtc cactgtttga gtggcagcgt ggtgaacgaa acacgcagtg taaacgcggt
    61621 attgcttaag ccgacaatcc cggcaaaatt cggttcacca ataatcagcc cgcgaatttc
    61681 ttcgttttcc attaattccg ctaccgcatc tttcagtgcc tgattggctt tatcagcatc
    61741 ttcatggcga tcaacatcat aatttgccac caccgaacca atgccgcgga caaagttagc
    61801 aaaggtggtt atcgaagacc acggaatgat gtgatacgcc ccggtatcct gtcgtacgcc
    61861 cacggagcga atcgacatcc gttccactgt gccggtcaac ggcccgatag tcaccaaatc
    61921 tccagtgttc atgccgtttt caaactgaat aaataccccg gtgataatat ctttcaccag
    61981 cgtttgcgaa ccaaacgaga tagccagacc taatgccccg gcacctgcca gcaatggcgc
    62041 gatattgacg ccgatttccg acaacacaat catgatggtg atggtactga taatcaccgc
    62101 cagcgcgtta cgaaacagcg taagcagggt acgcgtgcgg gcgctgggta gcgggcggcc
    62161 atgaatatcc gaagccagcc ggttttcgat caaactggcg agcaccgtcc agccaaccgc
    62221 cgagaagaaa agaatgagtg cgatacggat caggatatct acggttttct gccccgcgcc
    62281 gttttgcagc cagttccaga aatcgaacaa tccccatgcg ctcaacagca acattaccgc
    62341 cacgcagact gtcagaatac gcgccgtttt cagcgccgcc gacagccagc cattcaaccg
    62401 tttttgcagc tccggatagt tacgctgagt atgtggcgag agagtgatgg ttttcgccag
    62461 ccagcgggaa aacataccgg aaacaaacgc tgcgatacca ataatcgcca ggctgcgcac
    62521 cgttgcaccc atcataaatt tcaggctgtt gcccggatcg aacaacgaaa agaaaaacag
    62581 cacgataaaa taggcgcttg ccagccagtg ccacaccagc gcaaaggcgc ggataaacag
    62641 gctgaaaaag gccagcgaat gctccgcgaa gttgagcaaa tgctgggtaa tctctttttt
    62701 attacgaaag atcaggtaca acgcccagac ggtcatgcac agcataatga tgacgttcgc
    62761 cagcgcacct atctgtacat tcacctgatt agagataatc ggcacggcca caatcaggcc
    62821 ataacctatc aaactgctta accagctcag gcgacgactc cagtaacggg cgctctcgtc
    62881 ctgaatcgtg aacgggcgca gctccgccac gtttgggcaa aaaatcaggc gtagtacggc
    62941 tttgaaaaat tcaatgaggg caaaggcgtt gagaaacaaa ctttgttgga aagcgatggt
    63001 gcgactgcct gcattcaggt tgtcgcttaa tacctggccg acaaataatg tcagggccag
    63061 taacagcagg tcgataataa acgccccgat aatcatcgcc ggaagctgca accagttgct
    63121 gcgctcacga tttttttgcc gcgcccactg acccattttg cgatacagcg gcagtgcgca
    63181 caggcgaatc agccagtaaa aaccaaacac taataccgct aacattgaaa aatgggtcag
    63241 cgcattgctg aaggtttgtg gattaaacgg cttatgcggg gagccggtga tattgcgata
    63301 aagttgcccg aagcgggcgg aaagggcttc accataatgg cggctgactt cggtgacctt
    63361 ttgcagcacg gtttgctctt cgaccagcgt cggcggcact atttttggta ccggttcagc
    63421 agggggcgta gcggcaacgg tgcgcaactg gtcgatcaac tctttacgcg aggtgtcatt
    63481 atccagcaca tccgccagtg cgccataggc cgcttttttt tgttcgatat ccggttccgg
    63541 ggccggttca gtcgttgagt ccgttgtcgt tgtggttgta acgccgggta tggataccgc
    63601 gtgggcaggt gcgcccagca ggcagaagag gatgaacagg atccaccgca tgactcctcc
    63661 agtgagaaaa tagagcaaaa agataagtat agatgctgga ggaggggcga ttttaaatga
    63721 gaggaatctg gtgtgcctcc ctttcgggtg aaagggagga agggatatta agcaggttgc
    63781 tgacaacgtg cgggcttttt tgccggatgc ggcgtgaacg ccttatccgt cctacaagtt
    63841 catgcaattt taataagttg ccagatatcc tgtaggcctg ataagcgtag cgcatcaggc
    63901 aatgtgtctt attaagagac gtgctgcaaa aattcctgca agcgctggct cggcgggttc
    63961 ttgatcaaca cctgcggatt gccatcttcc gcaatccggc ctttgtcgat aaagatcagc
    64021 cgcgaagcta ctttctcggc aaaaccgatt tcgtgggtca cgatcaccat cgtcatccct
    64081 tcttcagcca gatcctgcat aaccttcagc acttcatggc gcagttccgg gtcaagagcg
    64141 gaagtcggtt catcaaacag catcattttc ggcttcaccg ccagcgcgcg ggcaatcgcc
    64201 acacgctgct gttgaccacc agaaagttcg gaagggtagt gatgtgcacg ttctgccaga
    64261 ccgactttcg ccagcagctc acgtgccagt ttttccgcct cttctttgtt cgcgccacgc
    64321 acgcgtagcg ggccaaacat gacgttttcc agcgctgtca gatgcgggaa gaggtaaaac
    64381 tgctggaaca ccatacctgc ttcctggcga atcaggcgct cgtcaacttt cggatcgtta
    64441 accttcaggc catcgacaat cagatcgccg gaggtgattt cttccagttt gttgatgcag
    64501 cgcagcaggg tcgatttacc ggaaccggac ggcccgataa tcaccacgac ttcgccctgg
    64561 gcaatgttca aatcgatatt gtgcagcacc tgggttgggc caaagtgctt ggagacgttt
    64621 ttaaattcaa tcacaggatt ttcatccttc tttccagacg acgcagaata aagctcagca
    64681 ccagggtaat aatcagatag aacaccgcca cggcgctcca gatctcaagg gcgcggaagt
    64741 taccggcaat aatttcttgc ccctgacggg tcagttccgc cacgccgatc acaataaaca
    64801 gcgaggtgtc tttaatgctg atgatccact ggttacccag cggcggcagc atacgacgca
    64861 gtgccagcgg taaaatgacg tagcgaatgg tttcccaacg tgaaagaccg agcgccagtc
    64921 ctgcttcacg aaaacctttg tggatagaca gcaccgcacc acgcgtgatt tccgcaatat
    64981 acgcgccgga gttgatcatg atggtgacca ccgccgcagt aaatgggtcg atgcgtaagt
    65041 cgttaaacgc catcggcagg gcgaaataaa taaacatcac ctggacgacg ataggtgtgc
    65101 cgcggatcac ttcaataaag accagcgcga cgtggttggc tatccaacct ccgaaggtgc
    65161 gtgcaaaacc tgccagcaat ccgattacca gaccgcctgc cagaccgagg accgaaatcc
    65221 acagggtcat tttggcacct tcaatcagaa gcggaatggc aggccagatg gcactccagt
    65281 caaactgcat atgttgttcc tgttaccgtg gtgtaaataa tcaaattcag ggtaattaat
    65341 ggccccggag cgggtttgcg cccctcaccc taatcctctc cccatagggg aatggtgaaa
    65401 ttttttcagt ttattgattt aattgaacta tatacgtttt tcttgaaaat atagccccca
    65461 atgtagcccc catcgtattg ttgttgtaca tggaggtaga ttgaactcag ttgaggcatt
    65521 atctgtaatg actcagcgaa tgcagtatac ctgtcggcct tgttcagggc gagacacaat
    65581 acccatgatg tgtaagttta tacagaagaa tggaacttta ccgaataatg tttcgccaga
    65641 acagcattca gcttctccac cagttccggg tgtttaaacg tgatatgcgc ggagcccttc
    65701 ctgaagtatc tgatgctgaa catctcatcc tcatagcacg ctttaccctg aacggaccgg
    65761 atgtgctcat ccagacgaat gataatattg tccctgtaat cgggtatggg tttgccgctg
    65821 aactgataca gtatcctttc caggtcggca agctgcccgg cctgcggacc atggttcaga
    65881 ccaaatcccc accagtccca tttcaccaga ttgttgagga taatcttact gccgaattta
    65941 cagggtgacc tgctgttgtg cgtccggctc agtcccctga agaggttgat gacgccgcgt
    66001 tcagccattt catacctgtt acggtgcagt tgttcagccg tgctcaggat attcgcttcg
    66061 ctgatttccg ggaaattatc ctcctccagt gaacggtacc attaatcacg cgtctgtaca
    66121 tccatcagac tcagcatccc ggagcgttgc atcaggtcac gccagatttc gcggtcaagg
    66181 ttacgggtga tggctttcat tgcggtttcc ggtttttcca tcagccagca gccacagcga
    66241 aaatcctgtt tcacggccca gtcccggaca gttttaccgc caatactttc tgtcattgct
    66301 gagatttctg ccagttgccg gataagcctt tcggtttgtt gcagtgcttc attccgtacc
    66361 gtcacaacgt gccggatatc tgtggagcag ataaggtccg tatgtccgtt aagcgcttcc
    66421 ggacgggaga tggtgatgtc tgacataact gtttcccttt tttctgataa atgaaaacgc
    66481 cagtcgtatt cggactggcg tcaggttaat gaatcgtggt gagtgagact caccggttct
    66541 ggtgcagaag ctgctgcagc tggcgcaggg tgcggatatc tgctctggcc tgttc
//
LOCUS       DBBGLQ010000021        66539 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.021, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000021 DBBGLQ010000000
VERSION     DBBGLQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.021"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(52..531)
                     /gene="folK"
                     /locus_tag="ACIYSB_001707"
     CDS             complement(52..531)
                     /gene="folK"
                     /locus_tag="ACIYSB_001707"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGC5428783.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGEMLRQILHTRAFDKLNKW"
     gene            complement(528..1925)
                     /gene="pcnB"
                     /locus_tag="ACIYSB_001708"
     CDS             complement(528..1925)
                     /gene="pcnB"
                     /locus_tag="ACIYSB_001708"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGC5428784.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1985..2881)
                     /gene="gluQRS"
                     /locus_tag="ACIYSB_001709"
     CDS             complement(1985..2881)
                     /gene="gluQRS"
                     /locus_tag="ACIYSB_001709"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGC5428785.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2948..3403)
                     /gene="dksA"
                     /locus_tag="ACIYSB_001710"
     CDS             complement(2948..3403)
                     /gene="dksA"
                     /locus_tag="ACIYSB_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGC5428786.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3581..4285)
                     /gene="sfsA"
                     /locus_tag="ACIYSB_001711"
     CDS             complement(3581..4285)
                     /gene="sfsA"
                     /locus_tag="ACIYSB_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGC5428787.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4300..4830)
                     /gene="thpR"
                     /locus_tag="ACIYSB_001712"
     CDS             complement(4300..4830)
                     /gene="thpR"
                     /locus_tag="ACIYSB_001712"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGC5428788.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4904..7333
                     /gene="hrpB"
                     /locus_tag="ACIYSB_001713"
     CDS             4904..7333
                     /gene="hrpB"
                     /locus_tag="ACIYSB_001713"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGC5428789.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIVPLLLQGSASPDARILLALLVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7529..10063
                     /gene="mrcB"
                     /locus_tag="ACIYSB_001714"
     CDS             7529..10063
                     /gene="mrcB"
                     /locus_tag="ACIYSB_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGC5428790.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRILPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10283..12526
                     /gene="fhuA"
                     /locus_tag="ACIYSB_001715"
     CDS             10283..12526
                     /gene="fhuA"
                     /locus_tag="ACIYSB_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGC5428791.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFSVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYYWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12577..13374
                     /gene="fhuC"
                     /locus_tag="ACIYSB_001716"
     CDS             12577..13374
                     /gene="fhuC"
                     /locus_tag="ACIYSB_001716"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGC5428792.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13374..14264
                     /gene="fhuD"
                     /locus_tag="ACIYSB_001717"
     CDS             13374..14264
                     /gene="fhuD"
                     /locus_tag="ACIYSB_001717"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGC5428793.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14261..16243
                     /gene="fhuB"
                     /locus_tag="ACIYSB_001718"
     CDS             14261..16243
                     /gene="fhuB"
                     /locus_tag="ACIYSB_001718"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGC5428794.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16401..17681)
                     /gene="hemL"
                     /locus_tag="ACIYSB_001719"
     CDS             complement(16401..17681)
                     /gene="hemL"
                     /locus_tag="ACIYSB_001719"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGC5428795.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17847..17894
                     /gene="yadX"
                     /locus_tag="ACIYSB_001720"
     CDS             17847..17894
                     /gene="yadX"
                     /locus_tag="ACIYSB_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGC5428796.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17906..19327
                     /gene="clcA"
                     /locus_tag="ACIYSB_001721"
     CDS             17906..19327
                     /gene="clcA"
                     /locus_tag="ACIYSB_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGC5428797.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19351..19416
                     /gene="yadW"
                     /locus_tag="ACIYSB_001722"
     CDS             19351..19416
                     /gene="yadW"
                     /locus_tag="ACIYSB_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGC5428798.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19409..19753
                     /gene="erpA"
                     /locus_tag="ACIYSB_001723"
     CDS             19409..19753
                     /gene="erpA"
                     /locus_tag="ACIYSB_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGC5428799.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19800..20423)
                     /gene="yadS"
                     /locus_tag="ACIYSB_001724"
     CDS             complement(19800..20423)
                     /gene="yadS"
                     /locus_tag="ACIYSB_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGC5428800.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20461..21261)
                     /gene="btuF"
                     /locus_tag="ACIYSB_001725"
     CDS             complement(20461..21261)
                     /gene="btuF"
                     /locus_tag="ACIYSB_001725"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGC5428801.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21254..21952)
                     /gene="mtnN"
                     /locus_tag="ACIYSB_001726"
     CDS             complement(21254..21952)
                     /gene="mtnN"
                     /locus_tag="ACIYSB_001726"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGC5428802.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22036..23553
                     /gene="dgt"
                     /locus_tag="ACIYSB_001727"
     CDS             22036..23553
                     /gene="dgt"
                     /locus_tag="ACIYSB_001727"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGC5428803.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23683..25107
                     /gene="degP"
                     /locus_tag="ACIYSB_001728"
     CDS             23683..25107
                     /gene="degP"
                     /locus_tag="ACIYSB_001728"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGC5428804.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25262..26419
                     /gene="cdaR"
                     /locus_tag="ACIYSB_001729"
     CDS             25262..26419
                     /gene="cdaR"
                     /locus_tag="ACIYSB_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGC5428805.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKTMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26508..26894)
                     /gene="yaeH"
                     /locus_tag="ACIYSB_001730"
     CDS             complement(26508..26894)
                     /gene="yaeH"
                     /locus_tag="ACIYSB_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGC5428806.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27056..27868)
                     /gene="yaeI"
                     /locus_tag="ACIYSB_001731"
     CDS             complement(27056..27868)
                     /gene="yaeI"
                     /locus_tag="ACIYSB_001731"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HGC5428807.1"
                     /translation="MISRRRFLQATAATIATSSGFGYMHYCEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMSLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27922..28746)
                     /gene="dapD"
                     /locus_tag="ACIYSB_001732"
     CDS             complement(27922..28746)
                     /gene="dapD"
                     /locus_tag="ACIYSB_001732"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGC5428808.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28777..31449)
                     /gene="glnD"
                     /locus_tag="ACIYSB_001733"
     CDS             complement(28777..31449)
                     /gene="glnD"
                     /locus_tag="ACIYSB_001733"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGC5428809.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDVDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVHNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGNPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31511..32305)
                     /gene="map"
                     /locus_tag="ACIYSB_001734"
     CDS             complement(31511..32305)
                     /gene="map"
                     /locus_tag="ACIYSB_001734"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGC5428810.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32673..33398
                     /gene="rpsB"
                     /locus_tag="ACIYSB_001735"
     CDS             32673..33398
                     /gene="rpsB"
                     /locus_tag="ACIYSB_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGC5428811.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33656..34507
                     /gene="tsf"
                     /locus_tag="ACIYSB_001736"
     CDS             33656..34507
                     /gene="tsf"
                     /locus_tag="ACIYSB_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGC5428812.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34654..35379
                     /gene="pyrH"
                     /locus_tag="ACIYSB_001737"
     CDS             34654..35379
                     /gene="pyrH"
                     /locus_tag="ACIYSB_001737"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGC5428813.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35671..36228
                     /gene="frr"
                     /locus_tag="ACIYSB_001738"
     CDS             35671..36228
                     /gene="frr"
                     /locus_tag="ACIYSB_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGC5428814.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36320..37516
                     /gene="ispC"
                     /locus_tag="ACIYSB_001739"
     CDS             36320..37516
                     /gene="ispC"
                     /locus_tag="ACIYSB_001739"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGC5428815.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37705..38463
                     /gene="ispU"
                     /locus_tag="ACIYSB_001740"
                     /gene_synonym="uppS"
     CDS             37705..38463
                     /gene="ispU"
                     /locus_tag="ACIYSB_001740"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGC5428816.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38476..39333
                     /gene="cdsA"
                     /locus_tag="ACIYSB_001741"
     CDS             38476..39333
                     /gene="cdsA"
                     /locus_tag="ACIYSB_001741"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGC5428817.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39345..40697
                     /gene="rseP"
                     /locus_tag="ACIYSB_001742"
     CDS             39345..40697
                     /gene="rseP"
                     /locus_tag="ACIYSB_001742"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGC5428818.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40727..43159
                     /gene="bamA"
                     /locus_tag="ACIYSB_001743"
     CDS             40727..43159
                     /gene="bamA"
                     /locus_tag="ACIYSB_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGC5428819.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPVISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43281..43766
                     /gene="skp"
                     /locus_tag="ACIYSB_001744"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43281..43766
                     /gene="skp"
                     /locus_tag="ACIYSB_001744"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGC5428820.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43770..44795
                     /gene="lpxD"
                     /locus_tag="ACIYSB_001745"
     CDS             43770..44795
                     /gene="lpxD"
                     /locus_tag="ACIYSB_001745"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGC5428821.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44900..45355
                     /gene="fabZ"
                     /locus_tag="ACIYSB_001746"
     CDS             44900..45355
                     /gene="fabZ"
                     /locus_tag="ACIYSB_001746"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGC5428822.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45359..46147
                     /gene="lpxA"
                     /locus_tag="ACIYSB_001747"
     CDS             45359..46147
                     /gene="lpxA"
                     /locus_tag="ACIYSB_001747"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGC5428823.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            46147..47295
                     /gene="lpxB"
                     /locus_tag="ACIYSB_001748"
     CDS             46147..47295
                     /gene="lpxB"
                     /locus_tag="ACIYSB_001748"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGC5428824.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47292..47888
                     /gene="rnhB"
                     /locus_tag="ACIYSB_001749"
     CDS             47292..47888
                     /gene="rnhB"
                     /locus_tag="ACIYSB_001749"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGC5428825.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47925..51407
                     /gene="dnaE"
                     /locus_tag="ACIYSB_001750"
     CDS             47925..51407
                     /gene="dnaE"
                     /locus_tag="ACIYSB_001750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGC5428826.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51420..52379
                     /gene="accA"
                     /locus_tag="ACIYSB_001751"
     CDS             51420..52379
                     /gene="accA"
                     /locus_tag="ACIYSB_001751"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGC5428827.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52478..54619
                     /gene="ldcC"
                     /locus_tag="ACIYSB_001752"
     CDS             52478..54619
                     /gene="ldcC"
                     /locus_tag="ACIYSB_001752"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGC5428828.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54676..55065
                     /gene="yaeR"
                     /locus_tag="ACIYSB_001753"
     CDS             54676..55065
                     /gene="yaeR"
                     /locus_tag="ACIYSB_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGC5428829.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCET
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            55130..56428
                     /gene="tilS"
                     /locus_tag="ACIYSB_001754"
     CDS             55130..56428
                     /gene="tilS"
                     /locus_tag="ACIYSB_001754"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGC5428830.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GLGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56477..56737)
                     /gene="rof"
                     /locus_tag="ACIYSB_001755"
     CDS             complement(56477..56737)
                     /gene="rof"
                     /locus_tag="ACIYSB_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGC5428831.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56724..56924)
                     /gene="yaeP"
                     /locus_tag="ACIYSB_001756"
     CDS             complement(56724..56924)
                     /gene="yaeP"
                     /locus_tag="ACIYSB_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGC5428832.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            57090..57635
                     /gene="yaeQ"
                     /locus_tag="ACIYSB_001757"
     CDS             57090..57635
                     /gene="yaeQ"
                     /locus_tag="ACIYSB_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGC5428833.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57632..58054
                     /gene="arfB"
                     /locus_tag="ACIYSB_001758"
     CDS             57632..58054
                     /gene="arfB"
                     /locus_tag="ACIYSB_001758"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGC5428834.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAMIKELTTEK
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            58068..58778
                     /gene="nlpE"
                     /locus_tag="ACIYSB_001759"
                     /gene_synonym="cutF"
     CDS             58068..58778
                     /gene="nlpE"
                     /locus_tag="ACIYSB_001759"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGC5428835.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58978..59802)
                     /gene="yaeF"
                     /locus_tag="ACIYSB_001760"
     CDS             complement(58978..59802)
                     /gene="yaeF"
                     /locus_tag="ACIYSB_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HGC5428836.1"
                     /translation="MDKPKAYCRLFLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(59856..61574)
                     /gene="proS"
                     /locus_tag="ACIYSB_001761"
     CDS             complement(59856..61574)
                     /gene="proS"
                     /locus_tag="ACIYSB_001761"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGC5428837.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61686..62393)
                     /gene="tsaA"
                     /locus_tag="ACIYSB_001762"
     CDS             complement(61686..62393)
                     /gene="tsaA"
                     /locus_tag="ACIYSB_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGC5428838.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62390..62794)
                     /gene="rcsF"
                     /locus_tag="ACIYSB_001763"
     CDS             complement(62390..62794)
                     /gene="rcsF"
                     /locus_tag="ACIYSB_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGC5428839.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62912..63727)
                     /gene="metQ"
                     /locus_tag="ACIYSB_001764"
     CDS             complement(62912..63727)
                     /gene="metQ"
                     /locus_tag="ACIYSB_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGC5428840.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63767..64420)
                     /gene="metI"
                     /locus_tag="ACIYSB_001765"
     CDS             complement(63767..64420)
                     /gene="metI"
                     /locus_tag="ACIYSB_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGC5428841.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64413..65444)
                     /gene="metN"
                     /locus_tag="ACIYSB_001766"
     CDS             complement(64413..65444)
                     /gene="metN"
                     /locus_tag="ACIYSB_001766"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGC5428842.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEVKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65632..66207
                     /gene="gmhB"
                     /locus_tag="ACIYSB_001767"
     CDS             65632..66207
                     /gene="gmhB"
                     /locus_tag="ACIYSB_001767"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGC5428843.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15702 a        16604 c        18324 g        15909 t
ORIGIN      
        1 agagatgaga tatgattttc agaaaatatt tatattcgca atataaataa attaccattt
       61 gtttaatttg tcaaatgctc ttgtatgtaa gatttgacgc aacatctccc catcaggaaa
      121 caccaactcc ggcgcgattt caaacagcgg ccacagcata aatccacgat tcttcatatc
      181 gtagtgcgga acggtcaggc gttcagtatt tatcacttca ttaccaaaca gcatgatgtc
      241 gagatccagc gtgcgtggtc cccagcgttc agctttgcgg acgcgacctt gctgcaattc
      301 aatacgctgt gtgtgattga gtagctcttc aggtgcaaga gaggtttcca gcgccacggc
      361 tgcgtttaag taatcgggtt gatcttgcgg ccccagcggt ggggtgcggt aaaacgaaga
      421 aacggtaaga atgtggcttt cagggatatc gcctaatgct ttcagggcag cattgacctg
      481 ctccagcgga gaggccagat tgctgcctat ggcaatatac gccactgtca tgcggtaccc
      541 tcacgacgtg gtgcgcgttt gcgtggacga cgagtacgac gacgcggtga cggttcttca
      601 tccagctcgt tgagcatccc tttttggtct ggtggcgcgg aaacctggaa ctcaccccac
      661 catttcacca gacgctgcag ttcagcgtta cgctcaactt cagctcgcaa ggccaacagg
      721 tcataagccg cacggaactt aggatgctcc agcagtttcc atgcgcgttt accctgacga
      781 cgggacatac gcaactgcaa ctgccagata tcgcgggtta atgtcgtcag acgtttcggg
      841 attgccagtg aacggcaggc ttcgtccagc acgtcgttca tcgccagcgc gaaagcgtcg
      901 tgataggtca ggccgctttc ctgggcgatc ttctgtgccg tctccagcag tgggtaccag
      961 aacatggcgg caaacaggaa cgccgggttc acgcgcatat cgttatggat acgcgtatcg
     1021 gtattcttca gcacctgttc aatgatccgc tccatcgggc tgtcgccatt ttccgtgaag
     1081 tagcgggtaa tggtcgggaa cagcggctgg aacagatgat attcacacaa cagcttatag
     1141 gtttcgtaac cgtagcccgc ttgtagcagt ttaagcgatt cttcaaacaa gcgtgccggt
     1201 gggatatcgt tcagcagggt agcgaggcga gggatcggtt ctgcggtttc cgggctgatg
     1261 cgcataccca atttggcggc aaaacgtacc gcgcgcagca tacgtaccgg atcttcacgg
     1321 tagcgcgttt ccgggttacc aatcagacgg ataacgccgt ccttcagatc cttcatgccg
     1381 ccaacgtaat cacggacggt aaaatccgct acgctgtaat acaggctgtt gatagtgaaa
     1441 tcgcggcgct gggcgtcttc ttcgatggag ccgaaaatgt tgtcgcgcag caacatgccg
     1501 ttttgcccgc gttgggaggt cgtgcggtcg ctgacgttac cttcgtggtg tccacggaag
     1561 gtcgcaactt cgataatctc cgggccaaac attacatgag ccagacggaa acggcgaccc
     1621 accaggcggc agttacggaa cagtttgcgc acctgctcag gcgtggcgtt agtggttacg
     1681 tcaaaatctt tcggcttttt gccaagtaac aggtcgcgca cgccgccgcc aaccagccag
     1741 gcttcgtatc ccgctttatt gagcctgtac attaccttca gggcattttc actgatatct
     1801 ttgcgggaaa tagcatgctg ctcacgcggg atcaccgtca cctgtggacg ggcgactgcc
     1861 tgttcagcct cgctttcctc gcggcttagc accttgcggc aaaaattagc gactcgggta
     1921 aaaatagtac acctcggtag tgtcaaacat cattcaggac aaaaaaatag cggctaatca
     1981 tagctcagca tgacgcattt gagaatgttg aatttacaat tgccgactcg ggcacggcgg
     2041 taagccgcca gtttttgacg gctgactgaa ggatttgctc gacgctgaaa tcctgccagt
     2101 gtgcttctgc ctgctgcccc agaaattgaa gtgccgcgat tagtaccggg cgtggatcgc
     2161 ctttcggcaa cgcaggcgca tgattctgct tggaaagttt agcgccttgt ggattaagcg
     2221 ccagcggcag atgaatgtaa tctggcactt tccagccaaa aagctggtac agcgagattt
     2281 gccttactgt tggttcaatc agatcagccc cacgcactat ttctgtaacg ccctggaaat
     2341 gatcatcaac cacaacagcc aggttgtagg cgaacaaccc atcacggcga tgaatgataa
     2401 aatcttcccg tgccagtttt tcgtcggcgt gaataatgcc gcgcagctgg tcagtaaatt
     2461 gcgtgaccgg atgctgctgg cggatacgca ctgcggcgtt gtctggtcca tgatgcaaca
     2521 cccggcaatg accgtcgtaa ataccgccaa tgctttgaat acgcgcacgc gtacaggtgc
     2581 agtaataact tagtccttgt tcatgtaacc aggcgagtgc ttcacgatag gcgtcgtgac
     2641 gttgcgattg ccagagaaca tcgccgtccc agtgcagacc gtaatgttcc agctggcgca
     2701 ggatagtttc tgcggcaccg ggaacttcac gaggcgggtc gatatcttct atgcgtacca
     2761 gccagcgacc ttgccgggcg cgagcctgca aatagctgcc gagcgcggcg atcagagagc
     2821 caaaatgaag ctcgccggaa ggagaggggg cgaagcggcc aatatactgt gtgtctgtca
     2881 tctctttgaa caaaaaataa ggcgggagca tttcccgcct gtggtaaacg tgatggaacg
     2941 gctgtaatta gccagccatc tgtttttcgc gaatttcagc cagcgttttg cagtcgatgc
     3001 acagatcggc tgtcgggcgc gcttccagac ggcgaatacc aatttcaaca ccgcaggatt
     3061 cgcagtagcc gaaatcttcg tcttccactt ttttcagcgt cttctcgatc tttttgatca
     3121 gcttacgctc gcgatcgcgg ttacgcagtt cgaggctgaa ctcttcttcc tgggctgcac
     3181 ggtctaccgg gtccgggaag ttggctgctt catcctgcat atgtgtaacg gtgcgatcga
     3241 cttcatccct gagttgatta cgccatgctt ccagaatacg acggaagtgc gccagctggg
     3301 cttcattcat atactcttcg cccggcttct cctgatatgg ttccacccca gcgatggcga
     3361 gaatactcag ggacgatgtt ttacggtttt gcccttcttg catgttgctt ctccttaaca
     3421 cgcactatcg atccccatgt tcgggggaaa aatgaggccg ctataaatag cagatgcttt
     3481 tccggatagc aattatctaa acgtaacact tgacaactgt gtgaggaaaa gcgtatttgc
     3541 gcacgcgacc agaatgtaaa ttaaccagtt acttacttta ctacaatgta accggcagtg
     3601 attttttaag agccatgcct tcagcagaaa tttccgcttt gtaagccaga atttctaccc
     3661 ccctctgttg agcttctgac aatagttgcg cgtatttctc atcgatgtgg cgcgcgggtg
     3721 aaaaccgtgt aatggctgaa tgcagcacgg cgaaaaagat aaccgcacgc tggccttcag
     3781 ccgctacgct catcaactcc cgaaggtgtt tctgacctcg ttcagtgacc gcatcgggaa
     3841 aatatccctg ttcgttctcc gctaacgtaa ccgatttcac ttcaatatag cagtctggac
     3901 gcgaatccgc ctgcaacata aagtcaatac ggctgcgttc tgcgccgtat tttacttcgc
     3961 ttttcagcga gctatagcct gacagctctg aaattgattc attaaggata gcctctttcg
     4021 tcaacctgtt agcccaaagc gtgttgacgc aaataaatgc gccgctctgg ctttgagtta
     4081 attcccaggt gtgtgggtat ttccgtttgg tgttgtctga agtcgaatac cagacggtat
     4141 cgccaggcgt tgcacaaccg gtcatcgcac ccgtattcgg gcagtgtagc gttaattcgc
     4201 gaccatcggg tgtgatcaca tcggctaaaa aacgtttgta acgctgaatt agcgtcgcgc
     4261 gctgtagagg gggagaaaat tccattgcga caatccttgt tattgcgtta gcgcccagcg
     4321 ttttagcggc gtgtagcgtg tgcgtccacg ggcaaacgag gaggcgtaaa gggtgaactc
     4381 cgtcaccgca tacgaccagt taaaacctgg cggcgggatt gtcaccgcct cgctggcgtc
     4441 gcgcaataag gtaatatgtg gatgaaacgg acgattgctt tgaaaacaac cgctgcgggc
     4501 agcctgtgaa cggagcatat tcgccagctg gattaagccg cgtggcggct gacgcatccc
     4561 taaccacacc acacgcgaac gcagccattg tccggcgtca tcaagcgtga gtgtgaaacc
     4621 aggttgacga atccgtccgg ctaaaagaga aagcgccttc tctttctcgg cgctcacttc
     4681 gcctaaaaat gccagagtca gatgcaaatt atcggcggcg accggacgtc ccgcctcagg
     4741 tgggaagtgt gtggcgcgcc aatggataat ctgttcgcgg atttctgcag gtaagtcgat
     4801 agcaaagaac agacgttgcg gttcagacat gtgaggtact cggttatgaa ttaccgcgat
     4861 gctacaatgt ggcgcgaaga atgttaaccc tctggagcgt tttgtgtcgt cgttgcccgt
     4921 tgctgccgtc ttacctgaat tacttaccgc tctcgattgt gcgccgcagg tattattaag
     4981 tgcgccgacc ggggccggga aatcaacctg gctgccgctg caactgctgg cgcatcccgg
     5041 cattaacggg aaaattatcc tgctggagcc gcgtcgtctg gcggcgcgta acgtcgcgca
     5101 acggctggcg gagctgctta acgaaaagcc aggcgatacc gttggctacc ggatgcgtgc
     5161 gcaaaactgc gtcgggccga atacccgcct ggaagtggtt accgaaggcg tgctgacgcg
     5221 catgatccag cgtgacccgg aactgagcgg tgttggactg gtgatccttg atgaatttca
     5281 tgagcgcagc ttgcaggcgg atttggcgtt ggcgctgtta ctcgatgtgc aacaaggtct
     5341 gcgtgatgac cttaaactgc tgattatgtc ggctaccctg gacaacgacc gcttgcagca
     5401 aatgctgcca gaagcgcctg tcgtcatctc agaagggcgc tcgtttccgg ttgaacgccg
     5461 ttatttaccg ctgcccgcgc atcagcgttt tgacgatgcc gttgcggtag ccaccgctga
     5521 aatgctgcgt caggaaagcg gatcattact gttattttta cctggcgtcg gagaaattca
     5581 gcgtgtgcag gaacaactgg cttcgcgcat cggcagtgat gtattgctct gcccgctgta
     5641 tggcgcgttg tcgctgaacg atcagcgaaa agcgatcctc ccggcaccgc aagggatgcg
     5701 caaagtggtg ctggcgacca atattgctga aaccagttta accattgaag gtattcgtct
     5761 ggtggtggat tgtgcccagg agcgtgtggc gcgttttgat ccgcgcacgg ggcttacgcg
     5821 actgattact caacgcgtta gccaggcatc catgacgcag cgtgccgggc gcgccgggcg
     5881 tctggagccg ggtatcagcc tgcatttaat cgccaaagaa caagcagaac gcgccgcggc
     5941 gcaaagtgaa ccggagatct tacaaagcga tctttccggt ttgctgatgg aattactgca
     6001 atggggatgc agcgatccgg cgcagatgag ctggctggat caaccgccag tagtgaatct
     6061 actggccgcg aaacgtctgt tacaaatgct gggggcactg gagggtgaac ggcttagtgc
     6121 gcaagggcaa aaaatggcag cgctgggtaa cgatccgcgt ttagcggcaa tgctggttag
     6181 cgcgaagaac gacgacgaag ctgctaccgc ggcaaaaatt gccgccattc tcgaagagcc
     6241 gccacggatg ggcaatagtg acctgggcgt ggcgttttcg cgcaatcaac cagcctggca
     6301 gcaacgtagt cagcaactgt taaaacgctt aaacgtacgt ggcggtgagg cagacagttc
     6361 gcttatcgcg ccgctacttg ccggggcgtt tgccgatcgc attgctcgtc gccgtgggca
     6421 agatggacgc tatcaactgg caaacggcat gggagcgatg ctcgatgcca acgacgcgct
     6481 aagccgccac gaatggttga tcgtaccgtt attattgcag ggcagcgcct cgccggatgc
     6541 gcggatttta ctggcgctgc tggtcgatat tgatgagtta gtacaacgct gcccgcagct
     6601 ggtacagcag tctgacactg tggagtggga tgacgcgcaa ggtacgctga aagcctggcg
     6661 tcggctacaa atcggtcagt tgacggtgaa agtgcagccg ctggcgaaac cgtcagaaga
     6721 cgagttgcat caggcgatgc ttaatggcat ccgtgataaa ggtttaagcg tgctcaactg
     6781 gacggcggaa gcggaacagc tacgcttgcg tttgttatgc gccgcaaagt ggttgccgga
     6841 atatgactgg ccagcggttg atgatgaaag tttattggca gcgctggaaa cgtggctgct
     6901 gccacatatg actggcgtac attcactacg cggcctgaaa tcactcgaca tttatcaggc
     6961 actacgcgga ttacttgatt ggggaatgca gcaacgtctg gatagtgaat tgcctgcgca
     7021 ttacactgtg ccgacgggaa gccggatcgc cattcgttat catgaagata acccgcccgc
     7081 gctggcggtg agaatgcaag agatgtttgg cgaggccacc aatccgacga tcgcccaggg
     7141 gcgcgtgccg ctggtgctgg agttgctttc acctgcccaa aggccattac aaatcacacg
     7201 agatttgagc gccttctgga aaggagcgta ccgtgaggtg caaaaagaga tgaaagggcg
     7261 ttatcccaaa catgtctggc cggacgaccc ggcaaatact gcaccgacgc gacggacgaa
     7321 aaagtattcg taaggttggt tttctccctc tccctgtggg agagggccgg ggtgagggca
     7381 tcagcgcgca cgttcaccct caccctaacc ctctccctca agggagaggg gaccgatcga
     7441 gcacaaattt tgagagatat cttcttctgt cttgtaacag aagaacagaa aatcgggctt
     7501 ttgcgcctga atattgcgga gaaaaagcat ggccgggaat gaccgcgagc caattggacg
     7561 caaagggaaa ccgacgcgtc cggtcaaaca aaaggtaagc cgtcgtcgtt acgaagatga
     7621 cgatgattac gacgattatg atgactatga ggatgaagaa ccgatgccgc gcaaaggtaa
     7681 gggcaaaggc aaagggcgta agcctcgtgg caaacgcggc tggctatggc tactgctaaa
     7741 actggctatc gtttttgccg tgctgatcgc catttacggc gtttatctcg atcaaaaaat
     7801 tcgtagccgt attgatggca aggtctggca actgcctgcg gcagtttatg gccgaatggt
     7861 caatcttgag ccagacatga ccatcagcaa gaacgagatg gtgaagctgc tggaggcgac
     7921 ccagtatcgt caggtgtcga aaatgacccg tcctggcgaa tttaccgtgc aggccaacag
     7981 cattgagatg attcgccgtc cgtttgattt cccggacagt aaagaaggac aggtgcgcgc
     8041 gcgtctgacc tttgatggcg atcatctggc gacgatcgtc aatatggaga acaaccgtca
     8101 gttcggtttc ttccgtcttg atccgcgtct gatcaccatg atctcttcgc caaacggtga
     8161 gcagcgtctg tttgtgccgc gcagtggttt cccggatttg ctggtggata ctttgctggc
     8221 gacagaagac cgtcattttt acgagcatga tggaatcagt ctctactcaa tcggacgtgc
     8281 ggtgctggca aacctgaccg ccggacgcac ggtacagggt gcgagtacgc tgacgcaaca
     8341 gctggtgaaa aacctgttcc tctccagcga gcgttcttac tggcgtaaag cgaacgaagc
     8401 ttacatggcg ctgatcatgg acgcgcgtta cagcaaagac cgtattcttg agctgtatat
     8461 gaacgaggtg tatctcggtc agagcggcga caacgaaatc cgcggcttcc cgctggcaag
     8521 cttgtattac tttggtcgcc cggtagaaga gctaagcctc gaccagcagg cgctgttagt
     8581 cggtatggtg aaaggggcgt ccatctacaa cccgtggcgt aacccaaaac tggcgctgga
     8641 gcgacgtaat ctggtgctgc gtctgctgca acagcaacag attattgatc aagaactcta
     8701 tgacatgttg agtgcccgtc cgctgggggt tcagccgcgc ggtggggtga tctctcctca
     8761 gccagccttt atgcaactgg tgcgtcagga gctgcaggca aaactgggcg ataaggtaaa
     8821 agatctctcc ggcgtgaaga tcttcactac ctttgactcg gtggcccagg acgcggcaga
     8881 aaaagccgcc gtggaaggca ttccggcact gaagaaacag cgtaagttga gcgatcttga
     8941 aactgcgatt gtggtcgtcg accgctttag tggtgaagtt cgtgcgatgg tcggaggttc
     9001 tgagccgcag tttgcgggct acaaccgtgc gatgcaggcg cgtcgttcga ttggttccct
     9061 tgcaaaacca gcgacttatc tgacggcctt aagccagccg aaaatctatc gtctgaatac
     9121 gtggattgcg gatgcgccaa ttgcgctgcg tcagccgaat ggccaggtct ggtcaccgca
     9181 gaatgatgac cgtcgttata gcgaaagcgg cagagtgatg ctggtggatg cgttgacccg
     9241 ttcgatgaac gtgccgacgg taaatctggg gatggcgctg gggctgcctg cggttacgga
     9301 gacctggatt aaactgggcg taccgaaaga tcagttgcat ccggttccgg caatgctgct
     9361 gggggcgttg aacttaacgc caatcgaagt ggcgcaggca ttccagacca tcgccagcgg
     9421 tggtaaccgt gcaccgcttt ctgcgctgcg ttcggtaatc gcggaagatg gcaaagtgct
     9481 gtatcagagc ttcccgcagg cggaacgcgc tgttccggcg caggcggcgt atctgacact
     9541 atggaccatg cagcaggtgg tacaacgcgg tacgggtcgt cagcttgggg cgaaataccc
     9601 gaacctgcat ctggcaggga aaacagggac taccaacaat aacgtagata cctggtttgc
     9661 gggcattgac ggcagcacgg tgaccatcac ctgggtcggc cgtgataaca accagccgac
     9721 caaactgtat ggtgccagcg gggcaatgtc gatttatcag cgttatctgg ctaaccagac
     9781 gccaacgccg ctgaatcttg ttccgccaga agatattgca gatatgggcg tggactacga
     9841 cggcaacttt gtttgcagcg gtggcatgcg tatcttgccg gtctggacca gcgatccgca
     9901 atcgctgtgc cagcagagcg agatgcagca gcagccgtca ggcaatccgt ttgatcagtc
     9961 ttctcagccg cagcaacagc cgcaacagca acctgctcag caagagcaga aagacagcga
    10021 cggtgtagcc ggttggatca aggatatgtt tggtagtaat taacatctaa gcgtgaaata
    10081 ccggatggcg agttgccatc cggtaaaata acatcccatc taagatatta accctttctt
    10141 ttcatctggt tgtttattaa cccttcagga acgctcagat tgcgtaccgc ttgcgaaccc
    10201 gccagcgttt cgaatattat cttatcttta taataatcat tctcgtttac gttatcattc
    10261 actttacatc agagatatac caatggcgcg ttccaaaact gctcagccaa aacactcact
    10321 gcgtaaaatc gcagttgtag tagccacagc ggttagcggc atgtctgttt atgcacaggc
    10381 agcggttgaa ccgaaagaag acactatcac cgttaccgct gcacctgcgc cgcaagaaag
    10441 cgcatggggg cctgctgcaa ctattgcggc gcgacagtct gctaccggca ctaaaaccga
    10501 tacgccgatt caaaaagtgc cacagtctat ttctgttgtg accgccgaag agatggcgct
    10561 gcatcagccg aagtcggtaa aagaagcgct tagctacacg ccgggtgtct ctgttggtac
    10621 gcgtggcgca tccaacacct atgaccacct gatcattcgc ggctttgcgg cagaaggcca
    10681 aagccagaat aactatctga atggcctgaa gttgcagggc aacttctata acgatgcggt
    10741 cattgacccg tatatgctgg aacgcgctga aattatgcgt ggcccggttt ccgtgcttta
    10801 cggtaaaagc agtcctggcg gcctgttgaa tatggtcagc aagcgtccga ccaccgaacc
    10861 gctgaaagaa gttcagttta aagccggtac tgacagcctg ttccagactg gttttgactt
    10921 tagcgattcg ttggatgatg acggtgttta ctcttatcgc ctgaccggtc ttgcgcgttc
    10981 tgccaatgcc cagcagaaag ggtcagaaga gcagcgttat gctattgcac cggcgttcac
    11041 ctggcgtccg gatgataaaa ccaattttac cttcctttct tacttccaga acgagccgga
    11101 aaccggttat tacggctggt tgccgaaaga gggaaccgtt gagccgctgc cgaacggtaa
    11161 gcgtctgccg acagacttta atgaaggggc gaagaacaac acctattctc gtaatgagaa
    11221 gatggtcggc tacagcttcg atcacgaatt taacgacacc ttttctgtgc gtcagaacct
    11281 gcgctttgct gaaaacaaaa cctcgcaaaa cagcgtttat ggttacggcg tctgctccga
    11341 tccggcgaat gcttacagca aacagtgtgc ggcattagcg ccagcggata aaggccatta
    11401 tctggcacgt aaatacgtcg ttgatgatga gaagctgcaa aacttctccg ttgataccca
    11461 gttgcagagc aagtttgcca ctggcgatat cgaccacacc ctgctgaccg gtgtcgactt
    11521 tatgcgtatg cgtaatgaca tcaacgcctg gtttggttac gacgactctg tgccactgct
    11581 caatctgtac aatccggtga ataccgattt cgacttcaat gccaaagatc cggcaaactc
    11641 cggcccttac cgcattctga ataaacagaa acaaacgggc gtttatgttc aggatcaggc
    11701 gcagtgggat aaagtgctgg tcaccctagg cggtcgttat tactgggcag atcaagaatc
    11761 tcttaaccgc gttgccggga cgaccgataa acgtgatgac aaacagttta cctggcgtgg
    11821 tggtgttaac tacctgtttg ataatggtgt aacaccttac ttcagctata gcgaatcgtt
    11881 tgaaccttct tcgcaagttg ggaaggatgg taatattttc gcaccgtcta aaggtaagca
    11941 gtatgaagtc ggcgtgaaat atgtaccgga agatcgtccg attgtagtta ctggtgccgt
    12001 gtataatctc actaaaacca acaacctgat ggcggaccct gagggttcct tcttctcggt
    12061 tgaaggtggc gagatccgcg cacgtggcgt agaaatcgaa gcgaaagcgg cgctgtcggc
    12121 gagtgttaac gtagtcggtt cttatactta caccgatgcg gaatacacca ccgatactac
    12181 ctataaaggc aatacgcctg cacaggtgcc aaaacacatg gcttcgttgt gggctgacta
    12241 caccttcttt gacggtccgc tttcaggtct gacgctgggc accggtggtc gttatactgg
    12301 ctccagttat ggtgatccgg ctaactcctt taaagtggga agttatacgg tcgtggatgc
    12361 gttagtacgt tatgatctgg cgcgagtcgg catggctggc tccaacgtgg cgctgcatgt
    12421 taacaacctg ttcgatcgtg aatacgtcgc cagctgcttt aacacttatg gctgcttctg
    12481 gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc tcttttgggg
    12541 cacggatttc cgtgcccatt tcacaagttg gctgttatgc aggaatacac gaatcattcc
    12601 gataccactt ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac gcttttgcat
    12661 ccgctgtcgt taacctttcc tgccgggaaa gtgaccggtc tgattggtca caacggttct
    12721 ggtaaatcca ctctgctcaa aatgcttggc cgtcatcagc cgccgtcgga aggggagatt
    12781 cttcttgatg cccaaccgct ggaaagctgg agcagcaaag cgtttgcccg caaagtggct
    12841 tatttgccgc agcagcttcc tccggcagaa gggatgaccg tgcgtgaact ggtggcgatt
    12901 ggtcgttacc cgtggcatgg cgcgctgggg cgctttgggg cggcagatcg cgaaaaagtc
    12961 gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc atcggctggt cgatagtctc
    13021 tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga tagccgttgt
    13081 ctgttgctcg acgaaccgac ctcggcgctg gatatcgccc accaggttga tgtgctgtcg
    13141 ctggtgcacc gtttaagtca ggagcgtggc ctgacggtca ttgccgtgtt gcacgatatc
    13201 aatatggcgg cacgctactg tgattatctg gtcgccctgc gcggcggtga aatgattgct
    13261 cagggaacgc ctgcggaaat tatgcgcggc gaaaccctcg aaatgattta tggcatcccg
    13321 atgggtattt tgccgcatcc ggcgggtgct gcacctgtga gttttgttta ttgatgagcg
    13381 gcttacctct tatttcgcgc cgtcgactgt taacggcgat ggcgctttct ccgttgttat
    13441 ggcagatgaa taccgcccac gcggcggcta ttgatcccaa tcgtattgtg gcgctggagt
    13501 ggttgccggt ggaattactg ctggcgctcg gcatcgtgcc ttacggcgtg gcggatacca
    13561 tcaactatcg cctgtgggtc agcgaaccac cattgccgga ctcagtgatc gacgtcggtt
    13621 tgcgcacaga acctaacctt gaactgctga ccgaaatgaa accatcgttt atggtctggt
    13681 cggcaggata tggcccttca ccagaaatgc tggctcgtat tgcgccgggt cgcggattta
    13741 acttcagtga cggcaaacag ccgttggcga tggcgcgtaa atcgctgacg gaaatggcag
    13801 atttacttaa cctgcaaagc gcagcggaaa cgcatttagc gcaatatgaa gactttatcc
    13861 gcagcatgaa accccgcttt gtgaagcgtg gtgcgcgtcc gttattgctg acgacgctta
    13921 tcgatccgcg ccatatgctg gtcttcggtc caaacagctt gttccaggaa attcttgatg
    13981 agtacggcat cccaaatgcc tggcaagggg aaaccaactt ctggggcagt accgccgtca
    14041 gtatcgatcg tctggcggcg tataaagacg ttgatgtgct ctgttttgat cacgacaaca
    14101 gcaaagacat ggatgcgcta atggcaacgc cgctgtggca ggccatgccg tttgtccgcg
    14161 ccggacgctt tcagcgcgta cctgcagtct ggttttatgg tgcgacgctc tcggcaatgc
    14221 actttgtgcg cgttctggat aacgccatcg gaggtaaagc gtgagtaaac gaattgcgct
    14281 tttcccggcg ttattgctgg cgctgttagt gattgtcgct acggcgctca cctggatgaa
    14341 cttctcgcag gcgctgccgc gtagccagtg ggcgcaggct gcctggtcgc cggatattga
    14401 cgtcatcgag cagatgattt ttcactacag cttgttgccg cgtctggcga tttcgctgct
    14461 ggtgggcgcg ggtctggggc tggtgggcgt gctgtttcag caagtgctgc gtaacccgct
    14521 ggcggagccg acgacgcttg gcgttgctac aggcgcgcaa ctggggatta ccgtcactac
    14581 gctctgggcg atccctggtg cgatggcgag ccagtttgct gcgctggcag gggcttgtgt
    14641 tgttggctta attgtctttg gcgtcgcgtg ggggaaacgg ctgtcgccgg taacgctgat
    14701 tctcgcgggg ttggtagtga gcctttattg cggcgcaatc aatcagttac tggttatctt
    14761 ccatcatgac caactgcaaa gcatgtttct gtggagcact ggaacgctga cgcaaaccga
    14821 ctggggcggc gttgagcgtt tatggccgca gctgctgggc ggtgtgatgc tgacgttgct
    14881 gctacttcgt ccgttaaccc tgatggggct tgatgatggc gtggcgcgca atctcgggct
    14941 ggccttgtcg cttgcgcgtc tggcggcgct gtcgctggcg attgtcatca gtgcgctgct
    15001 ggtgaacgct gtggggatta tcggctttat cgggttgttc gcgccgctgc tggcaaaaat
    15061 gctgggggcg cggcgtctgc tgccacgact gatgctggcg tcgttgattg gtgcgctgat
    15121 cctctggctt tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg aagtgtccac
    15181 cggttcggtc actgcgttga tcggtgcgcc gctgctactg tggctgttgc cgcgtttacg
    15241 cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac gccaacatgt
    15301 gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg tggcgctgtc
    15361 gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg aggatttaat
    15421 gccctggcgc tggccgcgaa ttatggcggc gctgtttgcg ggcgtcatgc tggcggtggc
    15481 gggctgtatt attcagcgac tgaccggaaa cccgatggca agcccggaag tgctggggat
    15541 tagctccggc gcggcgtttg gcgtggtgtt gatgctgttt ctggtgccgg gtaatgcctt
    15601 tggctggctg ttacctgcag gcagtctcgg cgcggcggtg acgctgttga tcattatgat
    15661 cgccgccggc cgcggtggat tttccccaca ccgtatgtta ctggcgggga tggcgttaag
    15721 caccgcgttc accatgcttt tgatgatgtt gcaggcaagt ggtgacccgc gaatggcgca
    15781 agtgctgacc tggatttccg gttcgaccta caacgcgacc gatgcgcagg tctggcgcac
    15841 cggaattgtg atggtgattt tgctggcgat taccccgctg tgccgccgct ggctgaccat
    15901 tttaccgctg ggtggtgata ccgcccgagc cgtaggaatg gcgctgacgc cgacgcgaat
    15961 tgcgctgctg ctgttagcgg cttgcctgac ggcgaccgcg acgatgacta ttggaccgtt
    16021 gagttttgtt ggtttaatgg caccgcatat tgcgcggatg atgggctttc gacggacgat
    16081 gccacacatc gtaatttcgg cgctggtggg tggtttactg ctggtgttcg ctgactggtg
    16141 tgggcggatg gtgctgtttc cattccagat cccggcgggg ctgctgtcaa cctttatcgg
    16201 cgcgccatat tttatctatt tgttgagaaa gcagagccgt taatttttcc atataacggc
    16261 cctgtacgcc tggacggata aagcgtttac gcgacaacgg atgcccgatg cgacgctggc
    16321 gcgtcttatc gggcctacaa acggccccga atcgtaggtc ggataaggcg ttcacgccgc
    16381 atccgacaaa ccatgctgga tcacaacttc gcaaacaccc gacgtgcagc atcgatggtg
    16441 ttattgatat cttccatgct gtgcgccacg gacataaagc ccgcttcaaa cgctgacggt
    16501 gccaggtaaa caccttcgtc cagcatcata tggaagaaac gcttaaagcg ttccacgtca
    16561 caggccatca catcctgata gcacgtcacg gactcggcgt cggtaaagaa aataccgaac
    16621 atgccgccaa cgtggttaac gaccagcgga attccggctt cttctgccgc ttccagcaga
    16681 ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt gaacgcccgg ctgcgcgact
    16741 tcattcagac aggcgaaacc cgctgccatc gcaatcgggt taccggaaag cgtacccgcc
    16801 tgatagaccg gacccgtcgg ggccagcgca tccattacat cacgacgacc accgaatgcg
    16861 cctaccggca ttccaccgcc gatgattttg ccgaggcagg ttaaatctgg cactacgccg
    16921 taataatcct gtgcgccagc tagcgctacg cggaaaccgg tcatcacttc atcgatgatc
    16981 agcaacgcgc caaattcgtc gcacagcgcg cgcagacctg gcaggaactc tggcagcggc
    17041 ggaacacagt tcatattgcc tgccaccggc tcgacgataa tacaggcaat ctcttgcggg
    17101 tattgctcaa atgcggcgcg tacagaagcc agatcattat aagtacaggt taaggtatgt
    17161 ttggcgaaat ctgccggaac gcccggcgag tttggctggc ctaacgtgag tgcgccagaa
    17221 ccggctttca ccagcaggca gtcagcgtga ccatggtaac acccttcaaa tttaataatt
    17281 ttgtcgcgac cggtaaaacc acgggccagg cggatggcgc tcatggtcgc ttcagtgccg
    17341 gagttcacca tgcgcaccat atccatggtc gggaccagtt cggtcaccag ttgcgccatt
    17401 ttcacttcca tttcggttgg tgcaccaaag cttaaaccac gctcggcggc ttcaatcacg
    17461 gcattgcgga ttgccggatg gttatggccc agcaccatcg gcccccagga accgacataa
    17521 tcgatatagg ctttgccatc aacatcgtac agataagcgc cgtccgcttt ttcgataaac
    17581 agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg agttcacacc gccagggatc
    17641 agctcgcgcg ctgcgctgta aagattttca gacttactca tggagggttc ctgattcgta
    17701 gaaaaagtga atggctgcta ttctatgtta ttcataacaa gttaaatact cgtcaaacat
    17761 caggctgctt gtactggtgc aatcctgatt tcttagagta taaaagtttt gtgcatttga
    17821 aacattacgc tttgcaaagg attttcatgg aacgtgcgag taaaatgccg tcatcttatt
    17881 tgtatgacca ataagtgatc attggatgaa aactgatact ccctctttag aaacaccgca
    17941 ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt cttgagcgcg ataaaacccc
    18001 gttagccatt ttgtttatgg cggcagtcgt cggcacgctt gttgggctgg cagcggttgc
    18061 ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg tacatactgc
    18121 tgataattat ccgcttctgt taaccgtcgc ttttctctgt tcggcggtgc tggcgatgtt
    18181 tggctacttt ttggtgcgca aatacgcgcc ggaagcaggt ggttcgggga tcccggaaat
    18241 tgaaggggcg ctggaagatc aacgtcccgt tcgctggtgg cgtgtattgc cggtgaagtt
    18301 ctttggcggg ctggggacac tcggcggagg catggtgttg gggcgcgaag ggccaaccgt
    18361 gcagatcggc ggtaacattg gccgtatggt gcttgatatt ttccgcctga aaggtgacga
    18421 agctcgccat acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg cctttaacgc
    18481 gccgctggcg ggtattttgt ttattatcga agagatgcgt ccgcagtttc gctatacgtt
    18541 aatttcgatt aaagcggtat ttattggtgt cattatgtcg accattatgt accggatttt
    18601 taatcatgaa gttgcgttga ttgacgtcgg taaactttct gatgcgccgc ttaatacgct
    18661 gtggctttat ctgatcctcg gtattatttt tggcattttc ggccctattt ttaataaatg
    18721 ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta ccaaatgggt
    18781 gctaatgggc ggtgcgattg gcggtctgtg tggattgctg gggtttgtgg caccagcaac
    18841 gtcgggcggc ggttttaacc tgattcctat cgctaccgcg gggaatttca gcatgggaat
    18901 gctggtgttt atcttcgtcg cgcgggtcat taccacctta ctctgcttct cttccggcgc
    18961 gccgggcggt atttttgccc cgatgctggc gctgggtact gtgctgggaa ccgctttcgg
    19021 aatggttgcc gttgagctgt ttccgcaata tcaccttgag gcggggacgt ttgctattgc
    19081 cggaatgggg gcattactgg cggcatctat tcgcgcgccg ttaacgggga tcattctggt
    19141 tctggagatg accgataact accagctcat tttgccaatg attattaccg gtcttggcgc
    19201 aacactatta gcgcaattta ccggcgggaa accgctatac tcggcgattc ttgcgcgcac
    19261 gctggcaaaa caggaagctg agcaactggc gcgaagcaag gccgcatcag ccagcgagaa
    19321 tacttgaacg aaataccagg gtattagata atggcgatta ttattgggtt agaatttgcc
    19381 caattgccga tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc cgctggagtt
    19441 taccgacgca gcagccaaca aagttaaaag cctgatcgct gacgaagata acccgaatct
    19501 gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg gtttcacctt
    19561 tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg gcctggtggt
    19621 tgatccgatg agcctgcaat atctggtcgg cggttccgtt gattataccg aaggtctgga
    19681 aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt gcggttcttc
    19741 ctttagtatc taatctgttg ttcatgtgcc ggataaacta tccggcacat cttctctctt
    19801 caacgcccat tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg aatagccgcc
    19861 agccgaatca atagcgtcac gaccatgccc atcatactgg ctgtttccag tggtacggaa
    19921 aatgtgtaat aagccgtagc gtggacaata ccgccgataa tacaggcagt tgcgtagatt
    19981 tctgtacgta aaatcatggg gatttcgcgg gccagaacat cacgaatgat cccgccgcca
    20041 acgccagtaa tgacgcccat acaaaccgcg attaacggac cggcttccgc attaaaggct
    20101 ttattcacgc caatgccgac aaacaccgcc agaccaacgg cgtccagcac cggcaacatc
    20161 cattttggta agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct ggtgaccatt
    20221 gcaacgacca gatcggtggg atctttcacc caaaataccg ggccgtgatc cagcgccatg
    20281 tcgcgaattg tcccgccgcc tactgcggtt accacgccca gtaccagaac accaaaaggg
    20341 tccatacgca attttccggc taacaaaacg ccggagatgg caaataccgc tgtgccgact
    20401 atatccagcc aatagacgag cattgttcaa tccccactga gcacctgaaa aggtcaggcg
    20461 ctaatctacc tgtgaaagcg cattacagag ctgttgtgca gcgaggataa tacgtgggct
    20521 tgcacgttca aaccagtcac tcgtgagagg aataacggga attttgagct gttcacccca
    20581 gtattgtttg attttaggaa tttggtccgg tccgcctgta atgacaatcg cctgtggcga
    20641 gcgtgctaac acctgttcgc ggctaacttg cggccaggga acccggctgt ctttaaagat
    20701 gttttctccg ccacaaactt cgagtacctg gttctgaatc gactcttttc cactggtaaa
    20761 tggcggatta atgccgaatt gcagaaaaac acgtttttta ggtttatcag catattgcgc
    20821 tttcaattgc gcgtactgat ccagcaggga ttgcgcggct tgttcggcct tgtctggttg
    20881 cggactccag ggggccagtt gacgtaacgc attggcaatt tgttcaatgc ttgtcgcatc
    20941 gacccacatc acttttattc ccagcgaagc cagctggtca acctgccgct cggcattacc
    21001 tccacgccag gcaatcacca gatcgggttt cagcgcgaca atgcgttcca gattcatccc
    21061 ctgccaggtg gaaacctgct caatcttttg cgcttgtgga ggatagtcgg aatagctgct
    21121 gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg gagaaagcgt
    21181 gatgacgcgc ggcgcggcgt tgagccacag tggcgcaaga aaagacaggg cgaccagcgc
    21241 cctgaacagt gacttagcca tgtgcaagtt tctgcaccag tgactcaacc atcaggctgg
    21301 actgtttagc ggcaacagcc aggaactcat cgaagctaag atgagactgt tgatcggcca
    21361 cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag acatgggcga
    21421 ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa gttgtggcgg attttcgcca
    21481 gaccaacaga accgttgatg aaagcgtcgc cgctaacaat caggccacgt acagcgttaa
    21541 gattcagttc ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca gctttaaagc
    21601 ctgccggaca gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca tccgcgtcgt
    21661 gataacgtgc ttcgtccgag acaacgatat cgcccacttt caacgttggt gccaggccac
    21721 cggcagaacc ggtgttaata atcacatctg gcttgcagtg ttccaacagc aaagtggcac
    21781 ccagcgccgc agcgacttta ccgatgcccg atttcagaag cgcaacctcg gttccattca
    21841 gttggccggt atagatttcg caaccgccga gactgatagt ttgacggttt tcgattttgt
    21901 cacgcagcag cgtaacttct tcttccattg caccaatgat gccgattttc atagatttac
    21961 tcgcgataag cccgatttga aggcatagtt taccatgcgc ttacggggaa gcgtatttct
    22021 cacgcgggag aggacatggc acagattgat ttccgaaaaa aaataaactg gcatcgtcgt
    22081 taccgttcac cgcagggcgt taaaaccgaa catgagatcc tgcggatctt cgagagcgat
    22141 cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac ccaggttttt
    22201 ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga agtccagcag
    22261 gtggggcgct acatcgccaa agaaatttta agccgtctga aagagcttaa attactggaa
    22321 gcatacggcc tggatgaact gaccggtccc tttgaaagca ttgttgagat gtcatgcctg
    22381 atgcacgata tcggcaatcc gccgtttggt cattttggcg aagcggcgat aaatgactgg
    22441 tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc ctctgactga cgatcgctgc
    22501 agcgtggcgg cactacgttt acgggacggg gaagaaccgc ttaacgagct gcggcgcaag
    22561 attcgtcagg acttatgtca ttttgagggg aatgcacaag gcattcgcct ggtgcataca
    22621 ttgatgcgga tgaatctcac ctgggcacag gttggcggta ttttaaaata tacccgtccg
    22681 gcgtggtggc gtggcgaaac gcctgagaca catcactatt taatgaaaaa gccgggttat
    22741 tatctttctg aagaagccta tattgcccgg ttgcgtaaag aacttaattt ggcgctttac
    22801 agtcgttttc cattaacgtg gattatggaa gctgccgacg acatctccta ttgtgtggca
    22861 gaccttgaag atgcggtaga gaaaagaata tttaccgttg agcagcttta tcatcatttg
    22921 cacgaagcgt ggggccagca tgagaaaggt tcgctctttt cgctggtggt tgaaaatgcc
    22981 tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg aagatcagtt ttttatgtat
    23041 ttacgggtaa acaccctaaa taaactggta ccctacgcgg cacaacgatt tattgataat
    23101 ctgcctgcga ttttcgccgg aacgtttaat catgcattat tggaagatgc cagcgaatgc
    23161 agcgatcttc ttaagctata taaaaatgtc gctgtaaaac atgtgtttag ccatccagat
    23221 gtcgagcagc ttgaattgca gggctatcgg gtcattagcg gattattaga gatttatcgt
    23281 cctttattaa gcctgtcgtt atcagacttt actgaactgg tagaaaaaga acgggtgaaa
    23341 cgtttcccta ttgaatcgcg cttattccac aaactctcga cgcgccatcg gctggcctat
    23401 gtcgaggctg tcagtaaatt accgtcagat tctcctgagt ttccgctatg ggaatattat
    23461 taccgttgcc gcctgctgca ggattatatc agcggtatga ccgacctcta tgcgtgggat
    23521 gaataccgac gtctgatggc cgtagaacaa taaccaggct tttgtaaaga cgaacaataa
    23581 atttttacct tttgcagaaa ctttagttcg gaacttcagg ctataaaacg aatctgaaga
    23641 acacagcaat tttgcgttat ctgttaatcg agactgaaat acatgaaaaa aaccacatta
    23701 gcactgagtg cactggctct gagtttaggt ttggcgttat ctccgctctc tgcaacggcg
    23761 gctgagactt cttcagcaac gacagcccag cagatgccaa gccttgcacc gatgctcgaa
    23821 aaggtgatgc cttcagtggt cagcattaac gtagaaggta gcacaaccgt taatacgccg
    23881 cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt ctccgttctg ccaggaaggt
    23941 tctccgttcc agagctctcc gttctgccag ggtggccagg gcggtaatgg tggcggccag
    24001 caacagaaat tcatggcgct gggttccggc gtcatcattg atgccgataa aggctatgtc
    24061 gtcaccaaca accacgttgt tgataacgcg acggtcatta aagttcaact gagcgatggc
    24121 cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct ctgatatcgc gctgatccaa
    24181 atccagaacc cgaaaaacct gaccgcaatt aagatggcgg attctgatgc actgcgcgtg
    24241 ggtgattaca ccgtagcgat tggtaacccg tttggtctgg gcgagacggt aacttccggg
    24301 attgtctctg cgctggggcg tagcggcctg aatgccgaaa actacgaaaa cttcatccag
    24361 accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc tggttaacct gaacggcgaa
    24421 ctgatcggta tcaacaccgc gatcctcgca ccggacggcg gcaacatcgg tatcggtttt
    24481 gctatcccga gtaacatggt gaaaaacctg acctcgcaga tggtggaata cggccaggtg
    24541 aaacgcggtg agctgggtat tatggggact gagctgaact ccgaactggc gaaagcgatg
    24601 aaagttgacg cccagcgcgg tgctttcgta agccaggttc tgcctaattc ctccgctgca
    24661 aaagcgggca ttaaagcggg tgatgtgatc acctcactga acggtaagcc gatcagcagc
    24721 tttgccgcac tgcgtgctca ggtgggtact atgccggtag gcagcaaact gaccctgggc
    24781 ttactgcgcg acggtaagca ggttaacgtg aacctggaac tgcagcagag cagccagaat
    24841 caggttgatt ccagctccat cttcaacggc attgaaggcg ctgagatgag caacaaaggc
    24901 aaagatcagg gcgtggtagt gaacaacgtg aaaacgggca ctccggctgc gcagatcggc
    24961 ctgaagaaag gtgatgtgat tattggcgcg aaccagcagg cagtgaaaaa catcgctgaa
    25021 ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac tcaacattca gcgcggcgac
    25081 agcaccatct acctgttaat gcagtaatct ccctcaaccc cttcctgaaa acgggaaggg
    25141 gttctcctta caatctgtga acttcaccac aactccatac atcttcatca tcctttaggc
    25201 atttgcacaa tgccgtacgt tacgtacttc cttatgctaa gccgtgcata acggaggact
    25261 tatggctggc tggcatcttg ataccaaaat ggcgcaggat atcgtggcac gtaccatgcg
    25321 catcatcgat accaatatca acgtaatgga tgcccgtggg cgaattatcg gcagcggcga
    25381 tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg gtactttcac agggacgagt
    25441 cgtcgatatc gatgacgcgg tagcacgtca tctgcacggt gtgcggcagg ggattaatct
    25501 accgttacgg ctggaaggtg aaattgtcgg cgtaattggc ctgacaggtg aaccagagaa
    25561 tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa atgatgctgg aacagtcgcg
    25621 gttgatgcac ttgttggcgc aggatagccg tttgcgggaa gaactggtga tgaacctgat
    25681 tcaggcagag gagaatactc ccgcacttac tgaatgggcg caacggctgg ggatcgatct
    25741 caatcaaccg cgagtggtgg ctattgttga ggtcgacagc ggtcagcttg gcgtggacag
    25801 cgcaatggcg gagttacaac aactgcaaaa cgcgctgact acgcccgagc gtaataatct
    25861 ggtggcgatt gtctcgctaa ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg
    25921 gcgctgggat gcagaagatc atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga
    25981 gtacggccag ctgcgttttc gcgtttcact gggcaactat tttaccggtc ctggcagtat
    26041 tgcccgatcc tatcgtacgg cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga
    26101 aagtcgctgc tatttttatc aggatctgat gttacctgtg ttactcgaca gtttgcgtgg
    26161 cgactggcag gccaacgaac tggcgcgacc gctggcgcgg ctgaaaacga tggacaataa
    26221 cggcttgctg cgacgaacgc tggcggcgtg gtttcgccac aatgtgcaac cgctggcaac
    26281 gtcaaaggcg ttgtttattc atcgtaatac cctggagtat cggcttaatc gtatatcgga
    26341 actgaccggg cttgatttgg gcaattttga tgacaggttg ctgctgtatg tggcgttaca
    26401 actggatgaa gagcggtagg ttatgcgtta aggttgaggc ggcgctggct cattcccctc
    26461 agcccgaagg gtagagggga atgcccgaat taaccgtcag ttcgggatta tttacgcgtt
    26521 agtttttcca gatcggcttc aatctcgctg atcttattgg ttacgactga ctccaggtga
    26581 cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc tgcggtcacg ctggcagatt
    26641 tgatcaagct catcaatgat ataccgtaga ttcgggctga tttcctggac ttctttataa
    26701 ccctgaccca caccatcagc gacgaccgtt ttacgctgac gcggatattt aaacttaacg
    26761 ctcttggcga aaaactcgcc tttgtctttc tggaaataga ttttcagaat atcgttgttg
    26821 gcttcctgcc ggaggctgta acgatcaatt tcttcaggat tggtaatacc cagacttttc
    26881 agattgtcgt acatagcgtt acctcaaaat gagtcagtaa attgtgctta ttttagcatt
    26941 tggcctggcc cgcccggctc gactgtgatc gcagaaagcc tttttcttcc agacttctat
    27001 catggcgtaa aaatcaaaaa attacctgct ttattctggt gataaaattc acgatctaca
    27061 ccagttccag catcgttact tccgggcggc agttcagacg caaaccatac aaactgccca
    27121 cgccacgggt tgtgtagatg tgtctttcgc caaaggcatt taatccggcg acgtaacgtt
    27181 tatcttcgac aggggcaaaa ggttcaccaa ccaacggtac gcgcagttgc ccgccgtgag
    27241 tatggccgca cagcatcaga tcccagggtt catcacgcat gacttctttg ctgtcgggat
    27301 tatgcgccag caccagtctt ggcagattgg cttcgctggc gggaggcggt ttgcattgtc
    27361 cggcccataa gtcaccagtg cctaccagtt cgaattgcct gttcggcgtg gcgatcaccg
    27421 tagcctggtt aaacaacacc gtgatgcccg ctgatttcaa cgtctcgcca attaagtgat
    27481 ttttttctgt accaacaggg cgatcgtggt tgccgaagca ggcaaacgtc ggcgcacatt
    27541 cggcaagggg ggagagtacg tcactaaacg ccgaaaaatt cagcgacata tcaaataata
    27601 cgtaatcgcc gcccagtaat atcaaatcgg gcttttgttc tatgccaaga gcaatcgcgt
    27661 cagaaatcag gcttaaagga acaaaacgag agtaatggag atcggccaga aaaagaattt
    27721 tgaatggtgc tgcgttgtct ttaaaaaagg cgaggcggtg acggattaat tcaaaccagc
    27781 ctggctcaca gtaatgcata taaccgaaac ctgagctcgt ggctatcgtc gcggcagtag
    27841 cctgcaaaaa tcggcggcgt gaaatcatcg ctcatccctg caaaaaaatc gggcagcgtc
    27901 gtgctgcccg tgtgcatact tttagtcgat ggtacgcagc agttcgttaa tgccgacttt
    27961 gccgcgagtt ttcgcgtcaa ctttcttaac gataaccgca cagtagaggc tgtatttgcc
    28021 atcttttgac ggcagattac ctgaaacaac cacagacccc gccggaacgc gaccgtagtg
    28081 gatttcgccg gtttcacggt cgtaaatacg ggtgctctga ccaatgtata cgcccatgga
    28141 aatgacggaa ccttcttcga caatcacccc ttcaaccact tcagagcgcg cgccgatgaa
    28201 gcaattatct tcaatgatgg ttgggttagc ctgcagcggt tccagcacgc cgccgatgcc
    28261 cacgccaccg gaaaggtgga cgtttttacc aatctgcgca caagaaccga cggtcgccca
    28321 ggtatcaacc atggtgcctt catcaacata tgcgccgatg ttgacgtaag acggcatcag
    28381 cacggtgtta cgggcaataa acgcaccctg acgtaccgcc gctggtggca caacgcggaa
    28441 gccttctttc tggaaacgtg cttcgtcgta gtcggcgaat ttcatcggca ctttgtcgaa
    28501 gtagcggctt tctgcccctt cgatcacctg attatcatta atacggaaag agagcagcac
    28561 cgcttttttc aaccactgat gcgtcaccca ctgaccgtca attttttccg ctacacgcag
    28621 tgcgccggaa tccagcaggg cgatcacctg atttaccgct tcgcgggtaa cggtgtctgc
    28681 attggctggc gtgatctcgg cacggcgttc aaaagcggtt tcaataatgt tctgtaactg
    28741 ctgcattgtt aaactctttt catatcagta aacacatcac cctttatcgt ttggattgag
    28801 ggcctctgtc aaccgctgat gcacttcctg ctgcaactcg ttattaagcg cacgccggtc
    28861 agcggtggca attatgaata aatcttctac tcgctcgcca atggttgtaa ttcgggcacc
    28921 atgaagcgaa attcccagat cggcaaaaat tttcccgact cgcgccagca gtccaggttg
    28981 gtcgagggcg atcagttcga ggaacgattt gcggtcggta tgggtcggca aaaacgttac
    29041 ttcggtttca acagtaaaat ggcgtaattt ggcgggttgg cgacggggct gcggtggctg
    29101 ccagctactt tgcgtcagta cttgctccag accaaaccga ataacctcat gacgatctgc
    29161 ggacagcggg ttgccatcgg gttccagcac gataaaggta tccatcgcca taccgtcgcg
    29221 agtggtgaaa atttgtgcgt cgtgaacact taaattgcgg cggtctaatt cggcacagac
    29281 ggcggcaaac agataagggc ggtccgggct ccagataaaa atctcggtgc ctccacgcgt
    29341 agcctgcggg ctaagcaata ccagcggttt gcttaaatca tgctgtaata aatggcgggc
    29401 atgccaggcc agttgatttg ggctatggcg gacaaaatag ttagcacgac agcgtgacca
    29461 aatttggtgc agcgcctctt cgtcgatgtt atccatgcgc agtagtgcca gtgcctggag
    29521 ttggtgatgg cgaacccgtt cgcgcatatc cggcgtgttt tgcattccgc gtcgtagctg
    29581 cttttcggtg gcaaagtaga gctcacgcaa cagactttgc ttccagctat tccacagcgt
    29641 ttcgttggtg gcgcaaatgt cagccacagt caggcatacc agatagcgca gacgattttc
    29701 cgtttgcact tcttcggcaa actgcttgat gacttccggg tcctgaatat cgcggcgttg
    29761 ggcggtcacc gacatcaaca ggtgctggcg aaccagccag gcgaccagct gtgtttcgcg
    29821 tgagttcagc ccgtggagtt cggcaaaatg cactacatcc tgagcaccga gaatggagtg
    29881 gtcgccgccg cgtcctttgg cgatatcgtg aaacagcgcg gcgatgaaaa tcagctcagt
    29941 tgacggcagg cgcggccaga cgtccacaca caacggatgg cgctggcgcg tttcttcact
    30001 ggcaaaactc tccagtttca gcatcacgcg gatagtatgt tcatccaccg tgtaggcgtg
    30061 gaacagatca aactgcatct gcccgacgat atgcgaccat tgcggcatat acgcgccgag
    30121 cacgctatgg cgatgcattg gcaatagccc gcgccgcacc gctccggggt gacgcagaat
    30181 gctcaaaaac agttttcgtg cttccggaat attacacagc ggttgttgca gatggcgacg
    30241 ggcatggcgt aactggcgca gcgtggtgga gtaaatgccg gtgatcgcac tgttgtgcac
    30301 catggtgtag aacatacgca agatggcttc cggctggcgc ataaatagtg tttcatcacg
    30361 caggtcgatt agcgtaccgc gtagctgaaa ctcatcgtcg attggacgtg gtttttcgtc
    30421 ggcgggaagg gcgaggatgg cttcatcgaa cagttgcagc agcatctggt tgagttcact
    30481 gacgcggcgt gtaacgcgga agtaatcctt catcatccgc tcgaccggtt cgttaccttc
    30541 accactgtaa ttcagacgct gggcgacgct aagctggcga tcgaataaca ggcgattatc
    30601 gtaacggctg acgaccagat gcagggcaaa gcgaatacgc cacaatatat gcagacattc
    30661 gtttaattcc gcccgctccg ctgaggttaa gaagccaaac ccgaccattt catccagcga
    30721 tgttgcgcca aaatgacggc gggccaccca ttgcagagtg tggatatcgc gcaagccgcc
    30781 agggctgctt ttgatgtctg gttcaaggtt gtagctggtg ccatggtaac gctgatggcg
    30841 ctggttctgt tcttcaactt tcgccgcgta gaacttgtcg gaaggccaga atccttcgct
    30901 gaaaatatgt ttttgcagtt cgaggaacag cgcaacatcg ccaattaata agcgggattc
    30961 gattaaattg gtggcgacgg ttaaatccga taacccttcc agcatgcact cttcaagcgt
    31021 gcgcacgcta tgaccgactt ccagctttac atcccagagc agcgttaaca gctcgcccac
    31081 tttttgcgcc tgatcgtccg ggagcttttt acggcttaaa atcagtaaat cgacgtctga
    31141 aagtggatgc agctcgccac gaccgtagcc accgacggcg accaatgcca ggtcggcaat
    31201 ctggctgaat cccgcttcaa tccataatcg ttgcaggagc tggtcgataa actcggtgcg
    31261 cgcctcaatc aactgttctg cagagatccc attgtcaaag gcatcaccca gccaacgctg
    31321 gaaagtatcg atatgggctt ttatcccacc gacggttaat tcatcacggg gccagacgca
    31381 tggattttgc ggttgaccgg gcagggtggg gagagcggtg tttgcgtact gttctggaag
    31441 ggtattcatt gtgcgccacc cataagatta aattatcgca ttaaaaaagc cggcattcgc
    31501 cggcttcatc ttattcgtcg tgcgagatta tcgccgggat ggtgtcatcc ttgcgtagcg
    31561 tcagaatttc gcagccgtta tcagtcacca caatagtatg ctcatattgt gcagacaagc
    31621 tgcgatcttt ggtttttacc gtccagccat ctttcatggt gcggatctct tttttacccg
    31681 cgttgaccat tggctcgatg gtgaacgtca tcccaggttt cagtacgacg ttggtttcac
    31741 gggagtcata gtgcagcacc tgcggttctt catggaagcc gcgaccaata ccgtgtccgc
    31801 aatattcacg aacgacggag aagccttctg cttcgacaaa tttctgaatc gccgcaccga
    31861 tttcgcgcag attaatgcct ggttttacca tgcgtagcgc caggtacagg ctttcttgcg
    31921 tgatgcggca cagacgttcg cccatgatgg tcggcttacc gacgataaac attttcgagg
    31981 tatcgccgtg gaaaccatct ttgattacgg tgacatcaat gttaacgata tcgccatctt
    32041 tcagcagctt agcatcgtcc gggataccgt ggcacaccac ttcattaata gagatgcaaa
    32101 cggatttcgg atagccgtga tagccgaggc aggcagaaac cgcgtgttgt tcattaacaa
    32161 tgtaatcatt acagatgcga tccagctcgc cggtgctgac gcccggttta acatacggtt
    32221 cgatcatctc cagcacttcg gcagccagtc ggccagcgac gcgcattttt tcgatatctt
    32281 ctggggtctt gattgagata gccattaatt ctgtccatca gcgtcggtga taccgacaat
    32341 atatatgtaa gtgccgtcaa tggtatcaca cccgggcaaa ttgagaatca ttctgaattt
    32401 cgccaaacgt gccactgaag gttttctata atagaaaatt cgacgtctga tgctgtacac
    32461 agcgccaaca attattggtg tccacgacgt atttgtggta taaagcgcgc cggacttccg
    32521 atccatttcg tatacacaga ctggacggaa gcgacaatct cactttgtgt aacaacacac
    32581 acgtatcggc acatattccg gggtgccctt tggggtcggt aatatgggat acgtggaggc
    32641 ataaccccaa cttttatata gaggttttaa tcatggcaac tgtttccatg cgcgacatgc
    32701 tcaaggctgg tgttcacttc ggtcaccaga cccgttactg gaacccgaaa atgaagccgt
    32761 tcatcttcgg tgcgcgtaac aaagttcaca tcatcaacct tgagaaaact gtaccgatgt
    32821 tcaacgaagc tctggctgaa ctgaacaaga ttgcttctcg caaaggtaaa atccttttcg
    32881 ttggtactaa acgcgctgca agcgaagcgg tgaaagacgc tgctctgagc tgcgaccagt
    32941 tcttcgtgaa ccatcgctgg ctgggcggta tgctgactaa ctggaaaacc gttcgtcagt
    33001 ccatcaaacg tctgaaagac ctggaaactc agtctcagga cggtactttc gacaagctga
    33061 ccaagaaaga agcgctgatg cgcactcgtg agctggagaa actggaaaac agcctgggcg
    33121 gtatcaaaga catgggcggt ctgccggacg ctctgtttgt aatcgatgct gaccacgaac
    33181 acattgctat caaagaagca aacaacctgg gtattccggt atttgctatc gttgatacca
    33241 actctgatcc ggacggtgtt gacttcgtta tcccgggtaa cgacgacgca atccgtgctg
    33301 tgaccctgta cctgggcgct gttgctgcaa ccgtacgtga aggccgttct caggatctgg
    33361 cttcccaggc ggaagaaagc ttcgtagaag ctgagtaata aggcttgata actcccccaa
    33421 aatagttcga gttgcagaaa ggcggcaagc tcgagaattc ccgggagctt acatcagtaa
    33481 gtgaccggga tgagcgagcg aagataacgc atctgcggcg cgaaatatga agggggagag
    33541 cccttataga ccaggtagta cacgtttggt tagggggcct gcatatggcc ccctttttca
    33601 cttttatatc tgtgcggttt aatgccgggc agatcacatc tccgaggatt ttagaatggc
    33661 tgaaattacc gcatccctgg taaaagagct gcgtgagcgt actggcgcag gcatgatgga
    33721 ttgcaaaaaa gcactgactg aagctaacgg cgacatcgag ctggcaatcg aaaacatgcg
    33781 taagtccggt gctattaaag cagcgaaaaa agcaggcaac gttgctgctg acggcgtgat
    33841 caaaaccaaa atcgacggca actacggcat cattctggaa gttaactgcc agactgactt
    33901 cgttgcaaaa gacgctggtt tccaggcgtt cgcagacaaa gttctggacg cagctgttgc
    33961 tggcaaaatc actgacgttg aagttctgaa agcacagttc gaagaagaac gtgttgcgct
    34021 ggtagcgaaa attggtgaaa acatcaacat tcgccgcgtt gctgcgctgg aaggcgacgt
    34081 tctgggttct tatcagcacg gtgcgcgtat cggcgttctg gttgctgcta aaggcgctga
    34141 cgaagagctg gttaaacaca tcgctatgca cgttgctgca agcaagccag aattcatcaa
    34201 accggaagac gtatccgctg aagtggtaga aaaagaatac caggtacagc tggatatcgc
    34261 gatgcagtct ggtaagccga aagaaatcgc agagaaaatg gttgaaggcc gcatgaagaa
    34321 attcaccggc gaagtttctc tgaccggtca gccgttcgtt atggaaccaa gcaaaactgt
    34381 tggtcagctg ctgaaagagc ataacgctga agtgactggc ttcatccgct tcgaagtggg
    34441 tgaaggcatc gagaaagttg agactgactt tgcagcagaa gttgctgcga tgtccaagca
    34501 gtcttaatta tcaaaaagga gccgcctgag ggcggcttct ttttgtgccc atcttgtaaa
    34561 ttcagctaac ccttgtgggg ctgcgctgaa aagcgacgta caatgtcgct agtattaatt
    34621 catttcaatc gttgacagtc tcaggaaaga aacatggcta ccaatgcaaa acccgtctat
    34681 aaacgcattc tgcttaagtt gagtggcgaa gctctgcagg gcactgaagg cttcggtatt
    34741 gatgcaagca tactggatcg tatggctcag gaaatcaaag aactggttga actgggtatt
    34801 caggttggtg tggtgattgg tgggggtaac ctgttccgtg gcgctggtct ggcgaaagcg
    34861 ggtatgaacc gcgttgtggg cgaccacatg gggatgctgg cgaccgtaat gaacggcctg
    34921 gcaatgcgtg atgcactgca ccgcgcctat gtgaacgctc gtctgatgtc cgctattcca
    34981 ttgaatggcg tgtgcgacag ctacagctgg gcagaagcta tcagcctgtt gcgcaacaac
    35041 cgtgtggtga tcctctccgc cggtacaggt aacccgttct ttaccaccga ctcagcagct
    35101 tgcctgcgtg gtatcgaaat tgaagccgat gtggtgctga aagcaaccaa agttgacggc
    35161 gtgtttaccg ctgatccggc gaaagatcca accgcaacca tgtacgagca actgacttac
    35221 agcgaagtgc tggaaaaaga gctgaaagtc atggacctgg cggccttcac gctggctcgt
    35281 gaccataaat taccgattcg tgttttcaat atgaacaaac cgggtgcgct gcgccgtgtg
    35341 gtaatgggtg aaaaagaagg gactttaatc acggaataat tcccgtgatg gataaataag
    35401 ggtaagattc cgcgtaagta tcgcgggggc gtaagtctgg ttataaggcg ttattgttgc
    35461 aggcagtttg gtcacggcca gcgcgcagca accggagcgt acaaaagtac gtgaggatgg
    35521 cgagcactgc ccggggccaa aatggcaaat aaaatagcct aataatccag acgattaccc
    35581 gtaatatgtt taatcagggc tatacttagc acacttccac tgtgtgtgac tgtctggtct
    35641 gactgagaca agttttcaag gattcgtaac gtgattagcg atatcagaaa agatgctgaa
    35701 gtacgcatgg acaaatgcgt agaagcgttc aaaacccaaa tcagcaaaat acgcacgggt
    35761 cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat attacggcac gccgacgccg
    35821 ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta cactgaaaat caacgtgttt
    35881 gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg cgtccgatct tggcctgaac
    35941 ccgaactctg cgggtagcga catccgtgtt ccgctgccgc cgctgacgga agaacgtcgt
    36001 aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag cgcgtgttgc agtacgtaac
    36061 gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga aagataaaga gatcagcgaa
    36121 gacgacgatc gccgttctca ggacgatgta cagaaactga ctgatgctgc aatcaagaaa
    36181 attgaagcgg cgctggcaga caaagaagca gaactgatgc agttctgatt tcttgaacga
    36241 caaaaacgcc gctcagtaga tccttgcgga tcggctggcg gcgttttgct ttttattctg
    36301 tctcaactct ggatgtttca tgaagcaact caccattctg ggctcgaccg gctcgattgg
    36361 ttgcagcacg ctggacgtgg tgcgccataa tcccgaacac ttccgcgtag ttgcgctggt
    36421 ggcaggcaaa aatgtcactc gcatggtaga acagtgcctg gaattctctc cccgctatgc
    36481 cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg atgctacagc aacagggtag
    36541 ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat atggcagcgc ttgaggatgt
    36601 tgatcaggtg atggcagcca ttgttggcgc tgctgggctg ttacctacgc ttgctgcgat
    36661 ccgcgcgggt aaaaccattt tgctggccaa taaagaatca ctggttacct gcggacgtct
    36721 gtttatggac gccgtaaagc agagcaaagc gcaattgtta ccggtcgata gcgaacataa
    36781 cgccattttt cagagtttac cgcaacctat ccagcataat ctgggatacg ctgaccttga
    36841 gcaaaatggc gtggtgtcca ttttacttac cgggtctggt ggccctttcc gtgagacgcc
    36901 attgcgcgat ttggcaacaa tgacgccgga tcaagcctgc cgtcatccga actggtcgat
    36961 ggggcgtaaa atttctgtcg attcggctac catgatgaac aaaggtctgg aatacattga
    37021 agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa gtgctgattc acccgcagtc
    37081 agtgattcac tcaatggtgc gctatcagga cggcagtgtt ctggcgcagc tgggggaacc
    37141 ggatatgcgt acgccaattg cccacaccat ggcatggccg aatcgcgtga actctggcgt
    37201 gaagccgctc gatttttgca aactaagtgc gttgacattt gccgcaccgg attatgatcg
    37261 ttatccatgc ctgaaactgg cgatggaggc gttcgaacaa ggccaggcag cgacgacagc
    37321 attgaatgcc gcaaacgaaa tcaccgttgc tgcttttctt gcgcaacaaa tccgctttac
    37381 ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg gatatgcgcg aaccacaatg
    37441 tgtggacgat gtgttatctg ttgatgcgaa cgcgcgtgaa gtcgccagaa aagaggtgat
    37501 gcgtctcgca agctgaggat aatccggcta cagagagtcg cgctatttgt tagcgtaggg
    37561 cttcagtgat atagtctgcg ccatctgatc gtaagtagtt ggctttataa ggtcaggtat
    37621 gccgtggttt tacacggctt ttttttgtat aggcttcagt attcctgagt accgtaaacc
    37681 ctgtcaggga ataaaaaacg cgtgatgttg tctgctactc aaccacttag cgaaaaattg
    37741 ccagcgcatg gctgccgtca tgttgcgatc attatggacg gcaatggtcg ctgggcaaaa
    37801 aagcaaggga agattcgtgc ctttgggcat aaagccgggg caaaatccgt ccgccgggct
    37861 gtctcttttg cggccaacaa cggtattgag gcgttaacgc tgtatgcctt tagtagtgaa
    37921 aactggaacc gaccagcgca ggaagtcagt gcgttaatgg aactgtttgt gtgggcgctc
    37981 gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc tgcgtattat tggcgatacc
    38041 agtcgcttta actcgcgttt gcaagaacgt attcgtaaat ctgaagcgct aacagccggg
    38101 aataccggtc tgacgctgaa tattgcggcg aactacggtg gacgttggga tatagtccag
    38161 ggagtcaggc aactggctga aaaggtgcag caaggaaacc tgcaaccaga tcagatagat
    38221 gaagagatgc taaaccagca tgtctgtatg catgaactgg cccctgtaga tttagtaatt
    38281 aggactgggg gggagcatcg cattagtaac tttttgcttt ggcaaattgc ctatgccgaa
    38341 ctttacttta cagatgttct ctggcccgat ttcgatgaac aagactttga aggggcgtta
    38401 aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg agcccggtga tgaaacagcc
    38461 tgatgggggt cgcttttgct gaagtatcgc ctgatatctg cttttgtgtt aatacccgtc
    38521 gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg ccattgtaac gctggtggtc
    38581 tgcatgctgg cagcgtggga atggggacag cttagcggtt ttaccactcg ttcgcagcga
    38641 gtatggttgg cggtgttatg cgggttattg ttggcgctga tgctttttct gttgccggaa
    38701 tatcaccgaa atattcatca accgctggtt gaaatctcac tttgggcttc gctgggttgg
    38761 tggattgtcg cgctattgct ggtgctgttt tacccaggtt ccgcagcaat ctggcgtaac
    38821 tctaaaacat tgcgccttat ttttggcgtg ctaaccattg ttcccttctt ctggggcatg
    38881 ctggcgttac gggcctggca ctatgacgag aatcattaca gtggcgcaat atggctgctc
    38941 tatgtcatga tcctggtatg gggcgctgac tccggcgcat atatgtttgg caaattgttt
    39001 ggtaaacata agctggcacc gaaggtttct ccgggtaaaa cctggcaagg ctttatcggt
    39061 ggactcgcta ctgcagcggt aatctcatgg ggttatggca tgtgggcgaa tctcgacgtc
    39121 gctcccgtca ccttactcat ttgctctatt gtcgcagcgt tagcctcagt gctcggcgat
    39181 ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg acagcggtca tttaattcca
    39241 ggacacggtg gtattttaga tcgtattgat agcctgacgg ctgcggtacc ggtctttgct
    39301 tgcttgttgt tactggtatt caggacgctt taacggaagg taatatgctg agttttctct
    39361 gggatttggc ttcgttcatc gttgcactgg gtgtacttat caccgtgcat gaatttggtc
    39421 atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg tttctcaata gggtttggta
    39481 aggcgctctg gcggcgaact gataagctcg gcaccgaata tgttatcgcc ctgatcccgt
    39541 tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc ggtcgttccg gaactccgcc
    39601 accatgcctt caataataaa tctgtcggcc aacgagcggc gattattgcc gcaggtccgg
    39661 ttgcaaactt catttttgct atctttgcct actggctggt ttttattatt ggtgtgcctg
    39721 gcgtacgtcc ggtggttggc gaaatagcag ccaattcgat agctgcggaa gcacaaattg
    39781 caccaggtac ggaactaaaa gccgtagatg gtatcgaaac gcctgattgg gatgccgtgc
    39841 gtttgcagtt ggtcgataaa attggcgatg aaagcaccac cattacagta gcgccatttg
    39901 gcagcgacca acggcgggat gtaaagctcg atttacgtca ctgggcgttt gagcctgata
    39961 aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg gccgcaaatt gaacctgtac
    40021 tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg tttgcaagca ggcgacagga
    40081 tcgttaaagt cgatggtcag cccttaacgc agtgggtgac ctttgtgatg cttgtccggg
    40141 ataacccggg taaatcctta gcgttagaaa tcgaaaggca ggggagtccc ttgtctttga
    40201 cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat tggttttgtc ggtattgagc
    40261 cgaaagtcat tcctttgcca gatgagtata aagttgtacg ccagtatggg ccgttcaacg
    40321 ccatcgtcga agccacggac aaaacgtggc agctgatgaa gctgacggtc agtatgctgg
    40381 gaaaattgat caccggtgat gtgaaactga acaacctcag tgggccgatc tctatcgcca
    40441 agggggctgg gatgacagcg gaactcgggg ttgtttatta cctgccgttt cttgcgctta
    40501 ttagcgtgaa cttagggata attaacctgt ttccgttgcc cgtacttgac ggggggcatc
    40561 tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt atccgagcgg gttcaagact
    40621 tttgttatcg cattggctcg attctgctgg tgctgttaat ggggcttgca cttttcaatg
    40681 atttctctcg gttatgagag ttagttagga agaacgcata ataacgatgg cgatgaaaaa
    40741 gttgctcata gcgtcgctgc tgtttagcag cgccaccgta tacggtgctg aagggttcgt
    40801 agtgaaagat attcatttcg aaggccttca gcgtgtcgcc gttggtgcgg ccctcctcag
    40861 tatgccggtg cgcacaggcg acacggttaa tgatgaagat atcagtaata ccattcgcgc
    40921 tctgtttgct accggcaact ttgaggatgt tcgcgtcctt cgtgatggtg atacccttct
    40981 ggttcaggta aaagaacgtc cgaccattgc cagcattact ttctccggta acaaatcggt
    41041 gaaagatgac atgctgaagc aaaacctcga ggcttctggt gtgcgtgtgg gcgaatccct
    41101 cgatcgcacc accattgccg atatcgagaa aggtctggaa gacttctact acagcgtcgg
    41161 taaatatagc gccagcgtaa aagctgtcgt gaccccgctg ccgcgcaacc gtgttgacct
    41221 aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag caaattaaca ttgttggtaa
    41281 ccatgctttc accaccgacg aactgatctc tcatttccaa ctgcgtgacg aagtgccgtg
    41341 gtggaacgtg gtaggcgatc gtaaatacca gaaacagaaa ctggcgggcg accttgaaac
    41401 cctgcgcagc tactatctgg atcgcggtta tgcccgtttc aacatcgact ctacccaggt
    41461 cagtctgacg ccagataaaa aaggtattta cgtcacggtg aacatcaccg aaggcgatca
    41521 gtacaagctt tctggcgttg aagtgagcgg caaccttgcc gggcactccg ctgaaattga
    41581 gcagctgact aagatcgagc cgggtgagct gtataacggc accaaagtga ccaagatgga
    41641 agatgacatc aaaaagcttc tcggtcgcta tggttatgcc tatccgcgcg tacagtcgat
    41701 gcccgaaatt aacgatgccg acaaaaccgt taaattacgt gtgaacgttg atgcgggtaa
    41761 ccgtttctac gtgcgtaaga tccgttttga aggtaacgat acctcgaaag atgccgtcct
    41821 gcgtcgcgaa atgcgtcaga tggaaggtgc atggctgggg agcgatctgg tcgatcaggg
    41881 taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc gataccgata cccaacgtgt
    41941 tccgggtagc ccggaccagg ttgatgtcgt ctacaaggta aaagagcgca acaccggtag
    42001 cttcaacttt ggtattggtt acggtactga aagtggcgtg agcttccagg ctggtgtgca
    42061 gcaggataac tggttaggta caggttatgc tgttggtatc aacgggacca aaaacgatta
    42121 ccagacctat gctgaactgt cggtaaccaa cccgtacttc accgtagatg gcgtaagcct
    42181 cggtggtcgt ctcttctata atgacttcca ggcagatgac gccgacctgt ccgactatac
    42241 caacaagagt tatggtacag acgtgacgtt gggcttcccg attaacgaat ataactcgct
    42301 gcgtgcaggt ctgggttatg tacataactc cctgtccaac atgcagcctc aggttgcgat
    42361 gtggcgttat ctgtactcta tgggtgaaca tccgagcacc tctgatcagg ataacagctt
    42421 caaaacggac gacttcacgt tcaactatgg ttggacctat aacaagcttg accgtggtta
    42481 cttcccgaca gatggttcac gtgtcaacct gaccggtaaa gtgaccattc ctggatcgga
    42541 taacgaatac tacaaagtga cgttagacac ggcgacttat gtgccgatcg atgacgatca
    42601 caaatgggtt gttctggggc gtacccgctg gggttatggt gatggtttag gcggcaaaga
    42661 gatgccgttc tacgagaact tctatgccgg tggttccagc accgtgcgtg gcttccagtc
    42721 caataccatt ggtccgaaag cagtttactt cccgcatcag gccagtaatt atgatccgga
    42781 ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac ctgtgtaaat cggatgatgc
    42841 tgtaggcggt aacgccatgg cggttgccag cctcgagttc atcaccccga cgccggttat
    42901 tagcgataag tatgctaact cggttcgtac ttccttcttc tgggatatgg gtaccgtttg
    42961 ggatacaaac tgggattcca gccaatattc tggatatccg gactatagtg atccaagcaa
    43021 tatccgtatg tctgcgggta tcgcattaca atggatgtcc ccattggggc cgttggtgtt
    43081 ctcctacgcc cagccgttca aaaagtacga tggagacaag gcagaacagt tccagtttaa
    43141 catcggtaaa acctggtaag tgttctccac aaaggaatgt agtggtagtg tagcgatgac
    43201 tttaggcgat caatataaga tcgccgggcc acgcaaagaa ctgcaccctc cggtgcaaat
    43261 gggatggtaa ggagtttatt gtgaaaaagt ggttattagc tgcaggtctc ggtttagcac
    43321 tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt caacatgggc agcctgttcc
    43381 agcaggtagc gcagaaaacc ggtgtttcta acacgctgga aaatgagttc aaaggccgtg
    43441 ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa aatgaaaaag ctgcagtcca
    43501 tgaaagcggg cagcgatcgc actaagctgg aaaaagacgt gatggctcag cgccagactt
    43561 ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg tcgttccaac gaagaacgcg
    43621 gcaaactggt tactcgtatc cagactgctg tgaaatccgt tgccaacagc caggatatcg
    43681 atctggttgt tgatgcaaac gccgttgctt acaacagcag cgatgtaaaa gacatcactg
    43741 ccgacgtact gaaacaggtt aaataagtaa tgccttcaat tcgactggct gatttagcgc
    43801 agcagttgga tgcagaacta cacggtgatg gcgatatcgt catcaccggc gttgcgtcca
    43861 tgcaatctgc acaaacaggt cacattacgt tcatggttaa cccaaaatac cgtgagcatt
    43921 taggcttgtg ccaggcgtcc gcggttgtca tgacccagga cgatcttcct ttcgcgaaaa
    43981 gtgccgcact ggtagtgaag aatccctacc tgacttacgc gcgcatggcg caaattttag
    44041 ataccacgcc gcagcccgcg cagaacattg cacccagtgc ggtgatcgac gcgacggcga
    44101 agctgggtaa caacgtatcg attggcgcta acgcggtgat tgagtccggc gttgaactgg
    44161 gcgataacgt gattatcggt gccggttgct tcgtaggtaa aaacagcaaa atcggtgcag
    44221 gttcgcgtct ctgggcgaac gtaaccattt accatgagat ccagatcggt cagaattgcc
    44281 tgatccagtc cggaacagtg gtaggcgcag acggctttgg ttatgccaac gatcgtggta
    44341 actgggtgaa gatcccacag attggtcgcg taattattgg cgatcgcgtg gagatcggtg
    44401 cctgcacaac catcgatcgc ggcgcgctgg atgacactat tattggcaat ggcgtgatca
    44461 ttgataacca gtgccagatt gcacataacg tcgtgattgg cgacaatacg gcggttgccg
    44521 gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta ctgcatgatc ggcggagcca
    44581 gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac ggttacgggc atgggtatgg
    44641 tgatgcgtcc catcactgaa ccaggcgtct attcctcagg cattccgctg caacccaaca
    44701 aggtctggcg caaaaccgct gcactggtga tgaacattga tgacatgagc aagcgtctga
    44761 aatcgcttga gcgcaaggtt aatcaacaag actaacgttc catcttttgt tcgccaaact
    44821 ttacggcctg tctcattctt acgattgcgg caggccgtgt tattattgtc gtttcttata
    44881 ttttgacagg aagagtatct tgactactaa cactcatact ctgcagattg aagagatttt
    44941 agaacttctg ccgcaccgtt tcccgttctt actggtggat cgcgtgctgg attttgaaga
    45001 aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat gagccattct tccagggcca
    45061 tttccctgga aaaccgattt tcccgggtgt gctgattctg gaagcaatgg cacaggcaac
    45121 aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg ggtgagctgt actacttcgc
    45181 tggtattgac gaagcgcgct tcaagcgccc ggtcgtgcct ggcgatcaaa tgatcatgga
    45241 agtcactttc gaaaaaacgc gccgcggcct gacccgtttt aaaggggttg ctctggtcga
    45301 tggtaaagta gtttgcgaag caacgatgat gtgtgctcgt agccgggagg cctgatacgt
    45361 gattgataaa tccgcctttg tgcatccaac cgccattgtg gaagagggcg cgtcaattgg
    45421 cgcgaacgca cacattggtc ctttttgtat cgttggaccc catgtcgaaa ttggtgaggg
    45481 taccgtactg aaatctcacg ttgtcgtgaa tggtcatact aaaattggcc gcgataatga
    45541 gatttatcag ttcgcctcca tcggcgaagt taaccaggat ctgaaatatg ctggcgaacc
    45601 gacccgtgtg gaaatcggcg atcgtaaccg cattcgcgaa agcgtcacca ttcatcgtgg
    45661 cacagtccag ggcggtggat tgacgaaggt gggcagcgac aacttactga tgatcaacgc
    45721 gcacattgcg cacgattgta cggtaggtaa ccgctgtatt ctcgccaaca acgcaacgct
    45781 ggcgggtcac gtatcggttg acgacttcgc gatcatcggc ggcatgaccg cagtccatca
    45841 gttctgcatc attggtgcgc acgtgatggt tggcggctgc tccggtgtgg cgcaggacgt
    45901 ccctccttat gtcattgcgc agggtaacca cgcaacgccg ttcggtgtca atatcgaagg
    45961 gctgaagcgc cgcggattca gccgtgaggc gattaccgct atccgcaatg cgtataagct
    46021 gatttatcgt agcggtaaaa cgctcgatga agtgaaaccg gaaattgctg aactggcgga
    46081 aacatatccg gaagtgaaag cctttaccga tttctttgca cgctcaacgc gcggtctgat
    46141 tcgttaatga ctgaacagcg tccattaacg attgccctgg tcgccggaga aacctccggc
    46201 gatatcctgg gggccggttt aatccgcgct ctgaaagaac atgtgcccaa cgcccgcttt
    46261 gttggtgttg ccgggccacg aatgcaggct gaaggctgcg aagcctggta cgaaatggaa
    46321 gaactggcgg tgatgggcat tgttgaagtg ctcggtcgtc tgcgtcgctt actgcatatt
    46381 cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag atgtttttgt tggtattgat
    46441 gcgcctgact tcaatattac tcttgaaggt aacctcaaaa agcagggtat caaaaccatt
    46501 cattacgtca gtccgtcagt ctgggcgtgg cgacagaaac gtgttttcaa aataggcaga
    46561 gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag cgttttatga caaatacaac
    46621 gtaccgtgcc gctttatcgg tcataccatg gctgatgcca tgccattaga tccagataaa
    46681 aatgccgccc gtgatgtgct ggggatccct cacgatgccc actgcctggc gttgctaccg
    46741 gggagccgtg gtgcagaagt tgaaatgctt agtgccgatt tcctgaaaac ggcccagctt
    46801 ttgcgccaga catatccgga tctcgaaatc gtggtgccac tggtgaatgc caaacgccgc
    46861 gagcagtttg aacgcatcaa agctgaagtc gcgccagacc tttcagttca tttgctggat
    46921 gggatgggcc gtgaggcgat ggtcgccagc gatgcggcgc tactggcgtc gggtacggca
    46981 gccctggagt gtatgctggc gaaatgcccg atggtggtgg gatatcgcat gaagcctttt
    47041 accttctggt tggcgaagcg gctggtgaaa actgattatg tctcgctgcc aaatctgctg
    47101 gcgggcagag agttagtcaa agaattattg caggaagagt gtgagccgca aaaactggct
    47161 gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc acgcgatgca cgataccttc
    47221 cgtgaactgc atcagcagat ccgctgcaat gccgatgagc aggcggcaca agccgttctg
    47281 gagttagcac aatgatcgaa tttgtttatc cgcacacgca gctggttgcg ggtgtggatg
    47341 aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc tgcggtgatc cttgacccgg
    47401 cgcgcccgat tgccgggctg aatgattcca aaaagctgag cgaaaaacgc cgtctggcgc
    47461 tctatgaaga gatcaaagag aaagcgttga gctggagtct gggccgcgcg gaaccccacg
    47521 aaatcgacga gctgaacatt cttcatgcga ccatgctggc gatgcagcgt gccgtcgctg
    47581 ggctgcatat tgcgccggaa tatgtgttga ttgatggtaa ccgctgcccg aaattaccga
    47641 tgcctgcgat ggctgtggtg aaaggcgata gccgcgtacc ggaaatcagt gccgcgtcta
    47701 tcctggcgaa agtgacgcgt gacgccgaaa tggcggcgct ggatattgtt ttcccgcaat
    47761 atggttttgc ccaacacaaa gggtacccaa ccgcttttca tctggaaaaa ctggctgaac
    47821 acggcgcgac cgaacaccat cggcgcagct ttgggcctgt caaacgcgca ctgggacttg
    47881 cgtcctgatt cttgtgtcga gattaagtaa accggaatct gaagatgtct gaaccacgtt
    47941 tcgtacacct gcgggtgcac agcgactact cgatgatcga tggcctggcc aaaaccgcac
    48001 cgttggtaaa aaaggcggcg gcgttgggta tgccagcact ggcgatcacc gatttcacca
    48061 acctttgtgg tctggtgaag ttctacggag cgggacatgg cgcagggatt aagcctatcg
    48121 tcggggcaga ttttaacgtc cagtgcgacc tgctgggtga tgagttaacc cacctgacgg
    48181 tactggcggc gaacaatacc ggctatcaga atctgacgtt gctgatctca aaagcgtatc
    48241 agcgcgggta cggtgccgcc gggccgatca tcgatcgcga ctggcttatc gaattaaacg
    48301 aagggttgat ccttctttcc ggcggacgca tgggcgacgt cggacgcagt cttttgcgtg
    48361 gtaacagcgc gctggtagat gagtgtgtcg cgttttatga agaacacttc ccggatcgct
    48421 attttctcga gctgatccgc accggcaggc cggatgaaga aagctatctg cacgcggcgg
    48481 tggaactggc ggaagcgcgc ggtttgcccg tcgtggcgac caacgacgtg cgctttatcg
    48541 acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat ccacgacggc tttaccctcg
    48601 acgatcctaa acgcccgcgt aactattcgc cgcagcaata tatgcgtagc gaagaggaga
    48661 tgtgtgagct gtttgccgac atccccgaag cccttgccaa caccgttgag atcgccaaac
    48721 gctgtaacgt aaccgtgcgt cttggtgaat acttcctgcc gcagttcccg accggggaca
    48781 tgagcaccga agattatctg gtcaagcgtg caaaagaggg cctggaagag cgtctggcct
    48841 ttttattccc tgatgaggaa gaacgtctta agcgccgccc ggaatatgac gaacgtctgg
    48901 agactgaact tcaggttatc aaccagatgg gcttcccggg ctacttcctc atcgttatgg
    48961 aatttatcca gtggtcgaaa gataacggcg taccggtagg gccaggccgt ggctccggtg
    49021 cgggttcact ggtggcctac gcgctgaaaa tcaccgacct cgatccgctg gaatttgacc
    49081 tgctgttcga acgtttcctt aacccggaac gtgtctccat gcctgacttc gacgttgact
    49141 tctgtatgga gaaacgcgat caggttatcg agcacgtagc ggacatgtac ggtcgtgatg
    49201 cggtatcgca gatcatcacc ttcggtacaa tggcggcgaa agcggtgatc cgcgacgtag
    49261 gccgcgtgct ggggcatccg tacggctttg tcgatcgtat ctcgaaactg atcccgcccg
    49321 atccggggat gacgctggcg aaagcgtttg aagccgagcc gcagctgccg gaaatctacg
    49381 aagcggatga agaagttaag gcgctgatcg acatggcgcg caaactggaa ggggtcaccc
    49441 gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc gaccaaaatt accgattttg
    49501 cgccgcttta ctgcgatgaa gagggcaaac atccggtcac ccagtttgat aaaagcgacg
    49561 ttgaatacgc cggactggtg aagttcgact tccttggttt gcgtacgctc accatcatca
    49621 actgggcgct ggagatgatc aacaagcggc gggcgaagaa tggcgagccg ccgctggata
    49681 tcgctgcgat cccgctggat gataagaaaa gcttcgacat gctgcaacgc tcggaaacca
    49741 cggcggtatt ccagcttgaa tcgcgcggca tgaaggacct gatcaagcgt ctacaacctg
    49801 actgcttcga agatatgatc gccctagtgg cactgttccg ccccggtccg ttgcaatcag
    49861 ggatggtgga taactttatc gaccgtaaac atggtcgtga agagatctcc tatccggacg
    49921 tacagtggca gcatgaaagc ctgaaaccgg tactggagcc aacctacggc attatcctgt
    49981 atcaggaaca ggtcatgcag attgcgcagg tgctttctgg ttataccctc ggtggcgcgg
    50041 atatgctgcg tcgtgcgatg ggtaagaaaa agccggaaga gatggctaag caacgttctg
    50101 tatttgctga aggtgcagaa aagaacggaa tcaacgctga actggcgatg aaaatcttcg
    50161 acctggtgga gaaattcgct ggttacggat ttaacaaatc gcactctgcg gcctatgctt
    50221 tggtgtcata tcaaacgtta tggctgaaag cgcactatcc tgcggagttt atggcggcgg
    50281 taatgaccgc cgatatggac aacaccgaga aggtggtggg tctggtggat gagtgctggc
    50341 ggatggggct gaaaatcctg ccaccagata taaactccgg tctttaccat ttccacgtca
    50401 acgacgacgg cgaaatcgtg tatggtattg gcgcgatcaa aggggtcggt gaaggtccga
    50461 ttgaggccat catcgaagcc cgtaataaag gcggctactt ccgcgaactg tttgatctct
    50521 gcgcccgtac cgacaccaaa aagttgaacc gtcgcgtgct ggaaaaactg atcatgtccg
    50581 gggcgtttga ccgtcttggg ccacatcgcg cagcgctgat gaactcgctg ggcgatgcgt
    50641 taaaagcggc agatcaacac gcgaaagcgg aagctatcgg tcaggccgat atgttcggcg
    50701 tgctggccga agagccggaa caaattgaac aatcctacgc cagctgccaa ccgtggccgg
    50761 agcaggtggt attagatggg gaacgtgaaa cgttaggcct gtacctgacc ggacacccta
    50821 tcaaccagta tttaaaagag attgagcgtt atgtcggagg cgtaaggctg aaagacatgc
    50881 acccgacaga acgtggtaaa gtcatcacgg ctgcggggct cgttgttgcc gcgcgggtta
    50941 tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct ggatgaccgt tccgggcggc
    51001 tggaagtgat gttgtttact gacgccctgg ataaatacca gcaattgctg gaaaaagacc
    51061 gcatacttat cgtcagcgga caggtcagct ttgatgactt cagcggtggg cttaaaatga
    51121 ccgctcgcga agtgatggat attgacgaag cccgggaaaa atatgctcgc gggcttgcta
    51181 tctcgctgac ggacaggcaa attgatgacc agcttttaaa ccgactccgt cagtctctgg
    51241 aaccccaccg ctctgggaca attccagtac atctctacta tcagagggcg gatgcacgcg
    51301 cgcggttgcg ttttggcgcg acgtggcgtg tctctccgag cgatcgttta ttaaacgatc
    51361 tccgtggcct cattggttcg gagcaggtgg aactggagtt tgactaatac aggaatacta
    51421 tgagtctgaa tttccttgat tttgaacagc cgattgcaga gctggaagcg aaaatcgatt
    51481 ctctgactgc ggttagccgt caggatgaga aactggatat taacatcgat gaagaagtgc
    51541 atcgtctgcg tgaaaaaagc gtagaactga cacgtaaaat cttcgccgat ctcggtgcat
    51601 ggcagattgc gcaactggca cgccatccac agcgtcctta taccctggat tacgttcgcc
    51661 tggcatttga tgaatttgac gaactggctg gcgaccgcgc gtatgcagac gataaagcta
    51721 tcgtcggtgg tatcgcccgt ctcgatggtc gtccggtgat gatcattggt catcaaaaag
    51781 gtcgtgaaac caaagaaaaa attcgccgta actttggtat gccagcgcca gaaggttacc
    51841 gcaaagcact gcgtctgatg caaatggctg aacgctttaa gatgcctatc atcaccttta
    51901 tcgacacccc gggggcttat cctggcgtgg gcgcagaaga gcgtggtcag tctgaagcca
    51961 ttgcacgcaa cctgcgtgaa atgtctcgcc tcggcgtacc ggtagtttgt acggttatcg
    52021 gtgaaggtgg ttctggcggt gcgctggcga ttggcgtggg cgataaagtg aatatgctgc
    52081 aatacagcac ctattccgtt atctcgccgg aaggttgtgc gtccattctg tggaagagcg
    52141 ccgacaaagc gccgctggcg gctgaagcga tgggtatcat tgctccgcgt ctgaaagaac
    52201 tgaaactgat cgactccatc atcccggaac cactgggtgg tgctcaccgt aacccggaag
    52261 cgatggcggc atcgttgaaa gcgcaactgc tggcggatct ggccgatctc gacgtgttaa
    52321 gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat gagctacggt tacgcgtaat
    52381 tcgcaaaagt tctgaaaaag ggtcacttcg gtggcccttt tttatcgcca cggtttgagc
    52441 aggctatgat taaggaagga ttttccagga ggaacacatg aacatcattg ccattatggg
    52501 accgcatggc gtcttttata aagatgagcc catcaaagaa ctggagtcgg cgctggtggc
    52561 gcaaggcttt cagattatct ggccacaaaa cagcgttgat ttgctgaaat ttatcgaaca
    52621 taaccctcga atttgcggcg tgatttttga ctgggatgag tacagtctcg atttatgtag
    52681 cgatatcaat cagcttaatg aatatctccc gctttatgcc ttcatcaaca cccactcgac
    52741 gatggatgtc agcgtgcagg atatgcggat ggcgctctgg ttttttgaat atgcgctggg
    52801 gcaggcggaa gatatcgcca ttcgtatgcg tcagtacacc gacgaatatc ttgataacat
    52861 tacaccgccg ttcacgaaag ccttgtttac ctacgtcaaa gagcggaagt acaccttttg
    52921 tacgccgggg catatgggcg gcaccgcata tcaaaaaagc ccggttggct gtctgtttta
    52981 tgattttttc ggcgggaata ctcttaaggc tgatgtctct atttcggtca ccgagcttgg
    53041 ttcgttgctc gaccacaccg ggccacacct ggaagcggaa gagtacatcg cgcggacttt
    53101 tggcgcggaa cagagttata tcgttaccaa cggaacatcg acgtcgaaca aaattgtggg
    53161 tatgtacgcc gcgccatccg gcagtacgct gttgatcgac cgcaattgtc ataaatcgct
    53221 ggcgcatctg ttgatgatga acgatgtagt gccagtctgg ctgaaaccga cgcgtaatgc
    53281 gttggggatt cttggtggga tcccgcgccg tgaatttact cgcgacagca tcgaagagaa
    53341 agtcgctgct accacgcaag cacaatggcc ggttcatgcg gtgatcacca actccaccta
    53401 tgatggcttg ctctacaaca ccgactggat caaacagacg ctggatgtcc cgtcgattca
    53461 cttcgattct gcctgggtgc cgtacaccca ttttcatccg atctaccagg gtaaaagtgg
    53521 tatgagcggc gagcgtgttg cgggaaaagt gatcttcgaa acgcaatcga cccacaaaat
    53581 gctggcggcg ttatcgcagg cttcgctgat ccacattaaa ggcgagtatg acgaagaggc
    53641 ctttaacgaa gcctttatga tgcataccac cacctcgccc agttatccca ttgttgcttc
    53701 ggttgagacg gcggcggcga tgctgcgtgg taatccgggc aaacggctga ttaaccgttc
    53761 agtagaacga gctctgcatt ttcgcaaaga ggtccagcgg ctgcgggaag agtctgacgg
    53821 ttggtttttc gatatctggc aaccgccgca ggtggatgaa gccgaatgct ggcccgttgc
    53881 gcctggcgaa cagtggcacg gctttaacga tgcggatgcc gatcatatgt ttctcgatcc
    53941 ggttaaagtc actattttga caccggggat ggacgagcag ggcaatatga gcgaggaggg
    54001 gatcccggcg gcgctggtag caaaattcct cgacgaacgt gggatcgtag tagagaaaac
    54061 cggcccttat aacctgctgt ttctctttag tattggcatc gataaaacca aagcaatggg
    54121 attattgcgt gggttgacgg aattcaaacg ctcttacgat ctcaacctgc ggatcaaaaa
    54181 tatgctaccc gatctctatg cagaagatcc cgatttctac cgcaatatgc gtattcagga
    54241 tctggcacaa gggatccata agctgattcg taaacacgat cttcccggtt tgatgttgcg
    54301 ggcattcgat actttgccgg agatgatcat gacgccacat caggcatggc aacgacaaat
    54361 taaaggcgaa gtagaaacca ttgcgctgga acaactggtc ggtagagtat cggcaaatat
    54421 gatcctgcct tatccaccgg gcgtaccgct gttgatgcct ggagaaatgc tgaccaaaga
    54481 gagccgcaca gtactcgatt ttctactgat gctttgttcc gtcgggcaac attaccccgg
    54541 ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac ggcgtttacc gcgtacgagt
    54601 cctaaaaatg gcgggataac ttgccagagc ggcttccggg cgagtaacgt gctgttaaca
    54661 aataaaggag acgttatgct gggtttaaaa caggttcacc atattgcgat tattgcgacg
    54721 gattatgcgg tgagcaaagc tttctactgc gatattcttg gtttcacgct gcaaagcgaa
    54781 gtctatcgcg aagcgcgcga ctcatggaaa ggggatttgg cgcttaatgg gcaatatgtg
    54841 attgagcttt tctcatttcc gttcccgccg gaacgaccca gccgaccgga agcttgcggt
    54901 ctgcgtcatc tggcttttag cgttgatgac atcgatgcgg cagtggcgca ccttgaaagc
    54961 cataacgtga agtgtgaaac catccgtgtc gatccataca cgcaaaaacg cttcaccttc
    55021 tttaacgatc cggacgggct gccgttggaa ctgtatgagc agtaaggctt gtcatcgccg
    55081 catttgcccg gtaacgtgcc gggcattgct actgtaaaat cgcaccatca tgacactcac
    55141 gctcaataga caacttctca cctcacgcca gattctggtg gcctttagcg gcgggcttga
    55201 ctccaccgtt ctgctgcatc agttggtgca gtggcggacg gaaaatccgg gtgtcgctct
    55261 gcgcgctatt catgtgcatc acggtttaag tgccaatgcc gatgcctggg ttacgcattg
    55321 cgaaaacgtc tgccaacagt ggcaggtgcc gctggtggtc gaacgcgtac aacttgcgca
    55381 agaaggactg ggcattgagg cccaggcgcg gcaggcacgt tatcaggcat ttgcccgcac
    55441 cttgttgccc ggtgaagtgc tggtcaccgc gcaacatctc gacgatcaat gtgaaacctt
    55501 tctgctggcg ctaaaacgcg gcagtggccc tgccgggctt tcggctatgg cggaagtctc
    55561 ggagtttgcc ggaacgcggc ttattcgccc gttgctcgcc cgcacgcggg gggaactggt
    55621 gcagtgggcg cgtcagtatg atttacgctg gattgaagac gaaagtaatc aggacgactc
    55681 atacgatcgt aactttctgc gcctgcgcgt agtgccgtta ttgcagcagc gttggccgca
    55741 ttttgccgaa gcaacggccc gcagcgccgc actttgtgct gaacaagaga gcctgctgga
    55801 tgaactgctg gcagatgatt tagcacactg tcaatcgccg caggggacgc tgcagattgt
    55861 gccaatgctg gcgatgagtg atgcccgccg cgcggcgatt atccgccgct ggctggcagg
    55921 gcagaatgca ccgatgcctt cccgcgacgc gttggtgagg atctggcagg aagtggcgct
    55981 ggcgcgggaa gatgcctcac cctgtttacg tttgggcgcg tttgaaatcc gacgctatca
    56041 gtcgcaactg tggtggatta aatccgtcac cgggcaaagc gaaaacattg tgccgtggca
    56101 gacgtggctt caaccgctgg aattaccggc ggggctggga agtgtacagc ttaatgcggg
    56161 aggcgatatt cgccctccgc gtgcagacga agcggtcagc gtgcgtttca aagcgccagg
    56221 attgctgcat attgtcgggc gtaacggcgg acgtaagcta aagaaaatct ggcaagagct
    56281 gggcgtgccg ccgtggctac gtgacaccac gccactgctg ttttatggcg aaacgctgat
    56341 tgcggcggca ggggtatttg tgacgcaaga aggtgtggct gaaggtgaga atggcgtaag
    56401 ttttgtctgg cagaaaacgc ttagttaagt gaaagccgga taagacgcat caaacgtcgc
    56461 atccggcgaa agtcaatcag gattcgctta ccaccaccgt accgatttcc gggtggctaa
    56521 agctggtaat tttatccaga cgcagctcgc gggtttcgcc agcggcctcg acgaccaggt
    56581 actccacatt tttgcgggag actaaatcac tggcttttgc ctgcaatttt tcgccatctt
    56641 tcagctcaag tgtcagcatt aaatgatgct ggcaggcgag ctcaagatta tcgtaatcat
    56701 cacaattgat tggttgatac gtatcattca ttgacataat cgctcaccag taagtttgcc
    56761 gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg cgtcgtcggc agccatttca
    56821 ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt atcctaagtc tccgctggcg
    56881 atttccgcgt accgcttgcg tattaactca caatattttt ccacatgccc tcctgtcagc
    56941 actctgactt aaccgtggat gcaagtctaa gcctacgaag ataaactctg tttcgcaagg
    57001 tgactatacc acactcattt ctgcaatatc agcgccgcaa ctgcacgtat tccgttacaa
    57061 tggcctcctg attcgaaagg agttttctta tggcgcttaa agcgacaatt tataaagcga
    57121 cggttaatgt ggccgatctc gaccgcaacc agtttctcga tgcctctctg acgctggcgc
    57181 gccatccttc agaaacccag gagcgtatga tgctgcgctt gctggcgtgg ctgaaatatg
    57241 ctgatgaacg tctgcaattt acccgtggtt tgtgtgccga tgatgagccg gaagcgtggc
    57301 tgcgtaacga tcacctgggc attgatttgt ggattgagct ggggctgccg gatgagcggc
    57361 ggattaagaa agcctgcacc caggccgcag aagtggcgct gtttacctat aatagtcggg
    57421 cggcgcaaat ctggtggcag caaaatcaga gcaaatgtgt gcagtttgcc aatctttccg
    57481 tctggtatct ggacgatgaa caactggcga aagtaagcgc ctttgccgat cgtaccatga
    57541 cgctgcaggc aacgattcag gatggcgtga tctggttatc ggatgataag aataatctgg
    57601 aagtgaactt aaccgcctgg caacaacctt catgattgtg atttcccgac atgttgctat
    57661 tcccgatggt gagcttgaga tcaccgccat tcgtgcgcag ggcgcgggcg ggcagcatgt
    57721 taataagacc tcaacggcta ttcatctgcg ttttgacatt cgggcgtcca gcctgccaga
    57781 gtattacaaa gagcgtctgc tcgccgccag ccatcatttg atcagcagtg atggcgtgat
    57841 tgtcattaag gcacaggaat accgcagtca ggaactgaac cgcgaagcag ctctggcccg
    57901 gctggtggct atgattaaag aattaacaac agaaaaaaaa gcccgacgac ccacgcggcc
    57961 cacccgtgca tcgaaagagc gcaggctggc atcgaaagca caaaaatcaa gcgtgaaggc
    58021 gatgcgcggc aaagtgcgca gcggtcggga ataaaaagaa ggaatggatg gtgaaaaaag
    58081 cgatagtgac agcgatggct gtaatcagcc tctttactct gatgggatgt aataatcggg
    58141 ccgaagtcga tacgctttct ccggcgcagg ctgccgaact gaaaccgatg ccgcaaagtt
    58201 ggcgcggcgt gctgccgtgt gccgattgcg aaggaatcga aacctctctg ttcctcgaaa
    58261 aagacggaac atgggtgatg aatgagcgtt atctcggtgc tcgtgaagaa ccttcctcct
    58321 tcgcttccta cggtacatgg gcgcgaaccg ctgacaagct ggtattaacc gacagcaaag
    58381 gtgaaaagtc atattatcgg gcgaaaggcg atgcgctgga gatgctcgat cgtgaaggca
    58441 atccgattga atcgcagttc aactatacgc tggaagcggc acaatccagt ttacctatga
    58501 cgccgatgac cctgcggggc atgtattttt atatggctga tgcggcgacc ttcactgatt
    58561 gcgcgaccgg aaaacgtttc atggtagcga ataacgcaga gctggagcgt agctacctgg
    58621 ctgcgcgcgg tcacagtgaa aaaccggtgt tactgtcagt agaaggtcac tttacgcttg
    58681 agggtaatcc ggataccggt gcgccgacta aagtattggc acccgatacg gcagggaaat
    58741 tttaccccaa ccaggattgc agtagtttgg ggcagtaacc cgtcttgaga cagaaacaaa
    58801 cgcaaaacag gccagaagga tatatttcaa cattttgaat ttgcacgttt tttgtaggcc
    58861 ggataaggcg tttacgccgc atccggcaat ggtgctcaac gcctgatgcg acgctggcgc
    58921 gtcttatcat gcctacaacc cccctcatac cttaaggcgg ataaggcatt tactttatca
    58981 ccgggtcaaa ccaacaaagc gacccgcctt gatataaatc ccaggcttca ggtgcccaac
    59041 atactgtaac tgcgtttctg gcttaaacgc agagacatca ccaatacgca tatgcatcag
    59101 atcggcaggg ctaatccagc ccgattgcgc cagtgtaagt ggatgcccgg ctttggcaaa
    59161 ggcatccgtg acaaattccg aacaaaacca cgactttttg tctccttcgc ccacactgct
    59221 taactgcgct ttcgccaggc cgctgacgca ctgttggcga aaatcctcgg agaacggatt
    59281 cagtgagcac atctggcgag tcaccataaa gggaataaat tcgacaatgc cgcgatagtt
    59341 ataaccgcta tctttgattt tattggcaaa cgcggtgata tctgtggctt gttgcggggt
    59401 aagatccggg actcgtaaga cgaaaagctt atcactatgc ttcatcgctt ttttaaggga
    59461 aacgatctgg acgccagcgc ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc
    59521 aacgtgactc acagaggaag tgctgaagac gcggattcca aatgaggtta ccccaaggct
    59581 tgaggagaac agcaaatcgc cgggtttgag atcaggtgcc gttatttctt ttattgattg
    59641 ttcggtgaaa gagctttgat gctggaattt gacagcccat gtttttgcct cggcatctac
    59701 tgctgtggct gatgggtctg gctggctgat atcaacggta caagccgaaa gtaaaagaaa
    59761 gctggggaga aacaggcggc agtacgcctt tggtttatcc attttataca atccatgtaa
    59821 aaaaagggcc ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga
    59881 tattcgacga tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat
    59941 tcgatatcgt cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc
    60001 agttccatat cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg
    60061 ataccttgtg cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat
    60121 ttgtgcatgt tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg
    60181 atgcctcgtt cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata
    60241 ccgtagcaac ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc
    60301 agtgcttcgg agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg
    60361 atcagcagcg taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct
    60421 gcaacttccg gggtagcgac atcgcgatcc cagttgatgc cgaagtagtg tttaccatcg
    60481 atgttagcac cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga
    60541 atcggcatgt ttaccggacc cagtgaaccc ggaccggctt taaccacggc acgaatttct
    60601 tcttcggtcg cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg
    60661 ttcagctcgt gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc
    60721 gctttaacca gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt
    60781 tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg
    60841 cgcggttctt tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg
    60901 tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg
    60961 ctgccgccga tagaaccggt gtcggcttgt acggcgcgga aatccagccc catgcggctg
    61021 aagattttgc tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa
    61081 gtatggaaag agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc
    61141 ggacgcactt cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg
    61201 taagagctaa gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt
    61261 acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa
    61321 cgaccactct cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgatcgca
    61381 ccggcgttgt tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg
    61441 gtcggcagcc aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc
    61501 agctgatggc tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat
    61561 tggctagtac gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc
    61621 tgggacaaaa aaaagtgatt tagtttacca gtgcaaaaga aatgtcaaaa gagaagggcg
    61681 tgaatttaac gcggttccag cgcaaagact tcaaaacctg cgtcggtgac gcgccagcga
    61741 acgttaaaat catgtagcca gacggcatag gttttgcccg tttcctcacc tttacgatag
    61801 gccgggcgcg ggtcctgcgc cagtacttcg cggataaaca gcgttaactg cggataacgc
    61861 ttctccagcg tcaaaagctg cttttcgacc tctgcggtaa aactcaccgc catctctgca
    61921 gctggcgcac tttgcgcata gctggcactg gcatcgggaa gcgattcggc aaaggggaga
    61981 tacggtttga tatccactac cggcgtacca tcgaccagat ccagactgcc gagcttcaga
    62041 atcacgctgt ctttatggca aacaacctct ttcagctcta ccagcgacat gccaattggg
    62101 ttagggcgga aagtagagcg tgttgcgaaa acccccattc tggcgttacc gccgaggcgc
    62161 ggtggacgca cagtcggacg ccagccgcct tccatcgttt gatgaaagac gaaaaggatc
    62221 cataaatggc tgaacgcttc caggccgcga acggcgtcgg cctggttgta gggagcaatg
    62281 agatgcagtt cgccgttggc gctttttacc agacctggct ggcgcggaac ggcgaacttt
    62341 tctttatagg gcgagcgaat aacgcctatt tgctcgaact ggaaactgct catttcgccg
    62401 taatgttaag cgcagaaccg atacatacag cctgacgata gcagcctggc gtaccgctgg
    62461 tgacttcgca gctatgcagt aatacagcat tggctttcat tttagaggcg ttgatttgca
    62521 tccgcttacg tgcggttgga atgctcggcg gagagtcctg attagaggcc tggcaagagt
    62581 cgccactgac ttcaccgaga tcgcggaacg gtttgccgac taattcttct gcattggtat
    62641 aaattcggac cggcgtggcg cgcggcgctt tcggttttgc aggctccgct ttcggctggg
    62701 gtgcagtgct ttgaacgggt tcgacagggg atctgcttaa catggaacag ccgcttagca
    62761 tgagtgctac taaacagatc ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa
    62821 cgtcaatatt gaatcaggag cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat
    62881 gatattacaa attgtggaaa cagcctaaaa attaccagcc tttaacagct ccgccgttaa
    62941 acactttgtt tgctgcttcg taaacttcgt cagactgata agcctggacg aatttcttca
    63001 cgttctcggc gtctttgtta tcttcacgcg tcacgatcag gtttacgtac ggggactctt
    63061 tatcttcaac aaagataccg tctttcgccg gagtcaggcc aatctggctg gcataggtgg
    63121 tattgataac tgccagagcg atttgcgcgt cgtccagaga acgcggcagt tgcggtgctt
    63181 ccagttcaac aattttcaga tttttggggt tctcaacaac atcaagaacg gtcggcagca
    63241 ggccaacgcc atctttcagt ttgatcaagc ccactttttg cagcagcagc agtgaacgac
    63301 caaggttagt tgggtcgttt ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg
    63361 atttgatttt cttggagtaa ccagcaatcg gataaacaaa agtgttgcct actgcgacca
    63421 gtttgtagcc acgatctttc agttgctgat caaggtacgg tttatgctgg aaggcgttgg
    63481 cgtcgatatc gcctttgctc aatgcttcgt ttggcagaac atagtcgttg aaggttacca
    63541 gctcaacgtc caggccatat ttgtctttcg caactttctg cgcgacttct gcaacctgct
    63601 gttcggcacc aacaatcacg ccgactttaa tgtggtttgg atctttttca tcctgaccgc
    63661 agcctaccag tgccagtgat ccgatcaggg ctcccactgc cgcaaaggtt ttgaatttga
    63721 acgccatacc ttattccttc ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga
    63781 cagcccggac gatgcggtcg cctgcgaact gaattaaata aaccagaatg accagcaata
    63841 ccagtaccgt attcatcacc gtcgcgttat agccgatgta gccatactga tagccaatct
    63901 gacctaaacc accggcaccg actgcaccac ccatcgcgga ataaccgacc agggtaatca
    63961 gggtgatagt tgccgcattc accagacccg gcagcgcttc cggtaacagc accttacgga
    64021 cgatctgcat cggcgtggca cccattgcgc gggaagcttc aattaacccg gttgggatct
    64081 ccagcagagc gttctcgacc atacgggcaa taaacggtgc tgcaccaacg gttaacggaa
    64141 caatcgctgc ctgcaaacca atcgatgtac cgacaataac gcgggtaaac ggaatcatcc
    64201 atacaagcaa gataatgaac gggatggaac ggaaaatgtt cacaatcgca gaaacggtac
    64261 gatacagctt cgcgttagca ataatttgcc ccggacgcgt gacataaagc agaacgccaa
    64321 ccggcagacc aatcacaaag ccaaaaaaac cggatacgaa ggtcattgcc agcgtttccc
    64381 atacgccacg aaccagcagc cacatcatcg gctcagacat aacccagtac ctctactttt
    64441 acatggtgtt cctgcagcca ggcaatggcg gcttgcgtat cttgttgtgt gccgtgcatt
    64501 tcagtcagca tgatgccgaa cttaacgcca ccggcgtaat ccatctgcgc gctaataatg
    64561 ttgttgttga cgttgaaacg acgcgcggtt tcagaaagca gtggggcatc gaccgattga
    64621 ccggtaaact ccagacgcag catcggcacg cagtcagtaa atggctccgc ttgcagacgt
    64681 tcctggtaat cttccgggat atccagatgc agggtcgact gaataaactt ctgcgccagc
    64741 ggcgttttcg gatgcgagaa cacttcactt accgtgtcct gctcgatcag ttctccattg
    64801 ctgatgaccg ccacgcaatc acaaatgcgc ttcacaacgt ccatttcgtg ggtgatcaac
    64861 agaatcgtca accccagacg gcggttgatg tctttcagca gttcgagaat agaacgtgtc
    64921 gttgccgggt ccagcgcgct ggtggcttca tcacacagca atactttggg attgctggct
    64981 aacgcacggg caattgccac acgttgtttc tgcccaccgg aaagattcga cgggtagcta
    65041 tcatgcttat cgccaagacc aaccaatgac agcaattccg tcacgcgacg tttgacctcg
    65101 tctttcggtg tgttgtccag ctccagcggc agagccacgt tgccaaaaac agtacgcgaa
    65161 gagagcaggt taaaatgctg gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg
    65221 gattctgaca gcgtggtcag ttcctggcca tcgaccagca cgctaccctc ggttgggcgc
    65281 tccagcaggt ttacacaacg tataagcgta ctcttacccg cgcctgaggc accgataacg
    65341 ccataaattt gtccagctgg cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg
    65401 gtgccctggt ggaacacttt ggtgatattc gaaagtttta tcattgatta tttattatcg
    65461 tcattaagtt agtcgtggca tctcgaatgc ctgaaacggg caacgccgtc aatgaaatgg
    65521 atgttaaggc atccagacgt ctaaatcaat caggtttatg cgaagagcac tttcttgcag
    65581 gtcgaaacat gcgatactag cgtcacatgc cttattaagg agctataaaa ggtggcgaag
    65641 agcgtacccg caatttttct tgaccgtgat ggcaccatta atgtcgatca cggctatgtc
    65701 catgagatcg acaactttga atttatcgac ggtgttattg acgccatgcg cgagctaaaa
    65761 aaaatgggct ttgcgctggt ggtagtaacc aaccagtctg gcattgctcg cggtaaattt
    65821 accgaagcac agtttgaaac gctgaccgag tggatggact ggtcgctggc ggaccgagat
    65881 gtcgatctgg atggtatcta ttattgcccg catcatccgc agggtagtgt tgaagagttt
    65941 cgccaggtct gcgattgccg caaaccacat ccggggatgc ttttgtcagc acgcgattat
    66001 ttgcatattg atatggccgc ttcttatatg gtgggcgata aattagaaga tatgcaggca
    66061 gcggttgcgg cgaacgtggg aacaaaagtg ctggtgcgta cgggtaaacc tattacgcct
    66121 gaagcagaaa acgcggcgga ttgggtgtta aatagcctgg cagacctgcc gcaagcgata
    66181 aaaaagcagc aaaaaccggc acaatgatta aaagatgagc ggttgaaata aaaatgcatt
    66241 tttccgcttg tcttcctgag ccgactccct ataatgcgcc tccatcgaca cggcggatgt
    66301 gaatcacttc acacaaacag ccggttcggt tgaagagaaa aatcctgaaa ttcagggttg
    66361 actctgaaag aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagccgcgtc
    66421 gcaactgctc tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct
    66481 taacgtcgca agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattac
//
LOCUS       DBBGLQ010000022        66017 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.022, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000022 DBBGLQ010000000
VERSION     DBBGLQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.022"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            296..2947
                     /gene="ppc"
                     /locus_tag="ACIYSB_001768"
     CDS             296..2947
                     /gene="ppc"
                     /locus_tag="ACIYSB_001768"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGC5428844.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            3129..4862
                     /gene="eptC"
                     /locus_tag="ACIYSB_001769"
     CDS             3129..4862
                     /gene="eptC"
                     /locus_tag="ACIYSB_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGC5428845.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            5077..5928
                     /gene="yijO"
                     /locus_tag="ACIYSB_001770"
     CDS             5077..5928
                     /gene="yijO"
                     /locus_tag="ACIYSB_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGC5428846.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(5915..6256)
                     /gene="frwD"
                     /locus_tag="ACIYSB_001771"
     CDS             complement(5915..6256)
                     /gene="frwD"
                     /locus_tag="ACIYSB_001771"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGC5428847.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(6258..7136)
                     /gene="pflC"
                     /locus_tag="ACIYSB_001772"
     CDS             complement(6258..7136)
                     /gene="pflC"
                     /locus_tag="ACIYSB_001772"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGC5428848.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGLQVTVGG"
     gene            complement(7102..9399)
                     /gene="pflD"
                     /locus_tag="ACIYSB_001773"
     CDS             complement(7102..9399)
                     /gene="pflD"
                     /locus_tag="ACIYSB_001773"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGC5428849.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRFGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(9450..9770)
                     /gene="frwB"
                     /locus_tag="ACIYSB_001774"
     CDS             complement(9450..9770)
                     /gene="frwB"
                     /locus_tag="ACIYSB_001774"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGC5428850.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(9785..10864)
                     /gene="frwC"
                     /locus_tag="ACIYSB_001775"
     CDS             complement(9785..10864)
                     /gene="frwC"
                     /locus_tag="ACIYSB_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGC5428851.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            11173..13674
                     /gene="ptsP"
                     /locus_tag="ACIYSB_001776"
     CDS             11173..13674
                     /gene="ptsP"
                     /locus_tag="ACIYSB_001776"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGC5428852.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQQQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13686..14348
                     /gene="fsa"
                     /locus_tag="ACIYSB_001777"
     CDS             13686..14348
                     /gene="fsa"
                     /locus_tag="ACIYSB_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGC5428853.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            14359..15462
                     /gene="gldA"
                     /locus_tag="ACIYSB_001778"
     CDS             14359..15462
                     /gene="gldA"
                     /locus_tag="ACIYSB_001778"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGC5428854.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            15737..16354
                     /gene="yijF"
                     /locus_tag="ACIYSB_001779"
     CDS             15737..16354
                     /gene="yijF"
                     /locus_tag="ACIYSB_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGC5428855.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFNWRMVGHYRYFVK"
     gene            complement(16381..17286)
                     /gene="yijE"
                     /locus_tag="ACIYSB_001780"
     CDS             complement(16381..17286)
                     /gene="yijE"
                     /locus_tag="ACIYSB_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGC5428856.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(17379..19559)
                     /gene="katG"
                     /locus_tag="ACIYSB_001781"
     CDS             complement(17379..19559)
                     /gene="katG"
                     /locus_tag="ACIYSB_001781"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGC5428857.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKFTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(19888..20778)
                     /gene="metF"
                     /locus_tag="ACIYSB_001782"
     CDS             complement(19888..20778)
                     /gene="metF"
                     /locus_tag="ACIYSB_001782"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGC5428858.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            20854..20988
                     /locus_tag="ACIYSB_001783"
     CDS             20854..20988
                     /locus_tag="ACIYSB_001783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5428859.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(21127..23559)
                     /gene="metL"
                     /locus_tag="ACIYSB_001784"
     CDS             complement(21127..23559)
                     /gene="metL"
                     /locus_tag="ACIYSB_001784"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGC5428860.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(23562..24722)
                     /gene="metB"
                     /locus_tag="ACIYSB_001785"
     CDS             complement(23562..24722)
                     /gene="metB"
                     /locus_tag="ACIYSB_001785"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGC5428861.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            24999..25316
                     /gene="metJ"
                     /locus_tag="ACIYSB_001786"
     CDS             24999..25316
                     /gene="metJ"
                     /locus_tag="ACIYSB_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGC5428862.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            25500..26108
                     /gene="yiiX"
                     /locus_tag="ACIYSB_001787"
     CDS             25500..26108
                     /gene="yiiX"
                     /locus_tag="ACIYSB_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGC5428863.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     TDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(26169..26381)
                     /gene="rpmE"
                     /locus_tag="ACIYSB_001788"
     CDS             complement(26169..26381)
                     /gene="rpmE"
                     /locus_tag="ACIYSB_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGC5428864.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            26584..28782
                     /gene="priA"
                     /locus_tag="ACIYSB_001789"
     CDS             26584..28782
                     /gene="priA"
                     /locus_tag="ACIYSB_001789"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGC5428865.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQHHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            28938..29963
                     /gene="cytR"
                     /locus_tag="ACIYSB_001790"
     CDS             28938..29963
                     /gene="cytR"
                     /locus_tag="ACIYSB_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGC5428866.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            30055..31014
                     /gene="ftsN"
                     /locus_tag="ACIYSB_001791"
     CDS             30055..31014
                     /gene="ftsN"
                     /locus_tag="ACIYSB_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGC5428867.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASSQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            31107..31637
                     /gene="hslV"
                     /locus_tag="ACIYSB_001792"
     CDS             31107..31637
                     /gene="hslV"
                     /locus_tag="ACIYSB_001792"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGC5428868.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            31647..32978
                     /gene="hslU"
                     /locus_tag="ACIYSB_001793"
     CDS             31647..32978
                     /gene="hslU"
                     /locus_tag="ACIYSB_001793"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGC5428869.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            33045..33971
                     /gene="menA"
                     /locus_tag="ACIYSB_001794"
     CDS             33045..33971
                     /gene="menA"
                     /locus_tag="ACIYSB_001794"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGC5428870.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            34064..34549
                     /gene="rraA"
                     /locus_tag="ACIYSB_001795"
     CDS             34064..34549
                     /gene="rraA"
                     /locus_tag="ACIYSB_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGC5428871.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(34634..34879)
                     /gene="zapB"
                     /locus_tag="ACIYSB_001796"
     CDS             complement(34634..34879)
                     /gene="zapB"
                     /locus_tag="ACIYSB_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGC5428872.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            35304..36149
                     /gene="glpF"
                     /locus_tag="ACIYSB_001797"
     CDS             35304..36149
                     /gene="glpF"
                     /locus_tag="ACIYSB_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGC5428873.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            36172..37680
                     /gene="glpK"
                     /locus_tag="ACIYSB_001798"
     CDS             36172..37680
                     /gene="glpK"
                     /locus_tag="ACIYSB_001798"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGC5428874.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            37815..38825
                     /gene="glpX"
                     /locus_tag="ACIYSB_001799"
     CDS             37815..38825
                     /gene="glpX"
                     /locus_tag="ACIYSB_001799"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGC5428875.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            38922..39668
                     /gene="fpr"
                     /locus_tag="ACIYSB_001800"
     CDS             38922..39668
                     /gene="fpr"
                     /locus_tag="ACIYSB_001800"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGC5428876.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPHCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESTIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(39673..40101)
                     /gene="uspD"
                     /locus_tag="ACIYSB_001801"
     CDS             complement(39673..40101)
                     /gene="uspD"
                     /locus_tag="ACIYSB_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGC5428877.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(40128..40427)
                     /gene="yiiS"
                     /locus_tag="ACIYSB_001802"
     CDS             complement(40128..40427)
                     /gene="yiiS"
                     /locus_tag="ACIYSB_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGC5428878.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAATSSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(40639..41079)
                     /gene="yiiR"
                     /locus_tag="ACIYSB_001803"
     CDS             complement(40639..41079)
                     /gene="yiiR"
                     /locus_tag="ACIYSB_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGC5428879.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            41180..41779
                     /gene="yiiQ"
                     /locus_tag="ACIYSB_001804"
     CDS             41180..41779
                     /gene="yiiQ"
                     /locus_tag="ACIYSB_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGC5428880.1"
                     /translation="MKPGCTLFFLLCSALTVTTAAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            41887..42654
                     /gene="tpiA"
                     /locus_tag="ACIYSB_001805"
     CDS             41887..42654
                     /gene="tpiA"
                     /locus_tag="ACIYSB_001805"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGC5428881.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(42709..43464)
                     /gene="cdh"
                     /locus_tag="ACIYSB_001806"
     CDS             complement(42709..43464)
                     /gene="cdh"
                     /locus_tag="ACIYSB_001806"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGC5428882.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVRKQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGRYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(43571..44560)
                     /gene="sbp"
                     /locus_tag="ACIYSB_001807"
     CDS             complement(43571..44560)
                     /gene="sbp"
                     /locus_tag="ACIYSB_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGC5428883.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(44880..45842)
                     /gene="pfkA"
                     /locus_tag="ACIYSB_001808"
     CDS             complement(44880..45842)
                     /gene="pfkA"
                     /locus_tag="ACIYSB_001808"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGC5428884.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(46023..46925)
                     /gene="fieF"
                     /locus_tag="ACIYSB_001809"
     CDS             complement(46023..46925)
                     /gene="fieF"
                     /locus_tag="ACIYSB_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGC5428885.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(47074..47574)
                     /gene="cpxP"
                     /locus_tag="ACIYSB_001810"
     CDS             complement(47074..47574)
                     /gene="cpxP"
                     /locus_tag="ACIYSB_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGC5428886.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            47724..48422
                     /gene="cpxR"
                     /locus_tag="ACIYSB_001811"
     CDS             47724..48422
                     /gene="cpxR"
                     /locus_tag="ACIYSB_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGC5428887.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            48419..49792
                     /gene="cpxA"
                     /locus_tag="ACIYSB_001812"
     CDS             48419..49792
                     /gene="cpxA"
                     /locus_tag="ACIYSB_001812"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGC5428888.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(49898..50572)
                     /gene="yiiM"
                     /locus_tag="ACIYSB_001813"
     CDS             complement(49898..50572)
                     /gene="yiiM"
                     /locus_tag="ACIYSB_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGC5428889.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(50721..51704)
                     /gene="kdgT"
                     /locus_tag="ACIYSB_001814"
     CDS             complement(50721..51704)
                     /gene="kdgT"
                     /locus_tag="ACIYSB_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGC5428890.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(51964..52584)
                     /gene="sodA"
                     /locus_tag="ACIYSB_001815"
     CDS             complement(51964..52584)
                     /gene="sodA"
                     /locus_tag="ACIYSB_001815"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGC5428891.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIMGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            52869..53903
                     /gene="rhaT"
                     /locus_tag="ACIYSB_001816"
     CDS             52869..53903
                     /gene="rhaT"
                     /locus_tag="ACIYSB_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HGC5428892.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKSLIIHNVLLSTLGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(53900..54748)
                     /gene="rhaR"
                     /locus_tag="ACIYSB_001817"
     CDS             complement(53900..54748)
                     /gene="rhaR"
                     /locus_tag="ACIYSB_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGC5428893.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(54822..55658)
                     /gene="rhaS"
                     /locus_tag="ACIYSB_001818"
     CDS             complement(54822..55658)
                     /gene="rhaS"
                     /locus_tag="ACIYSB_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGC5428894.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            55946..57415
                     /gene="rhaB"
                     /locus_tag="ACIYSB_001819"
     CDS             55946..57415
                     /gene="rhaB"
                     /locus_tag="ACIYSB_001819"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGC5428895.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQEQQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            57412..58671
                     /gene="rhaA"
                     /locus_tag="ACIYSB_001820"
     CDS             57412..58671
                     /gene="rhaA"
                     /locus_tag="ACIYSB_001820"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGC5428896.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSRRG"
     gene            58754..59578
                     /gene="rhaD"
                     /locus_tag="ACIYSB_001821"
     CDS             58754..59578
                     /gene="rhaD"
                     /locus_tag="ACIYSB_001821"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGC5428897.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            59588..59902
                     /gene="rhaM"
                     /locus_tag="ACIYSB_001822"
     CDS             59588..59902
                     /gene="rhaM"
                     /locus_tag="ACIYSB_001822"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGC5428898.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            60203..60649
                     /gene="frvA"
                     /locus_tag="ACIYSB_001823"
     CDS             60203..60649
                     /gene="frvA"
                     /locus_tag="ACIYSB_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGC5428899.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            60660..62111
                     /gene="frvB"
                     /locus_tag="ACIYSB_001824"
     CDS             60660..62111
                     /gene="frvB"
                     /locus_tag="ACIYSB_001824"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGC5428900.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            62101..63171
                     /gene="frvX"
                     /locus_tag="ACIYSB_001825"
     CDS             62101..63171
                     /gene="frvX"
                     /locus_tag="ACIYSB_001825"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGC5428901.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYEALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            63171..64919
                     /gene="frvR"
                     /locus_tag="ACIYSB_001826"
     CDS             63171..64919
                     /gene="frvR"
                     /locus_tag="ACIYSB_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGC5428902.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQVISADNVQGLLQRVPGIASLNIIDAQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(64969..>66017)
                     /gene="yiiG"
                     /locus_tag="ACIYSB_001827"
     CDS             complement(64969..>66017)
                     /gene="yiiG"
                     /locus_tag="ACIYSB_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGC5428903.1"
                     /translation="NLLSSAIIVAIMSLGLTGCDDKKAETETLPPANSQPAAPAPEAK
                     PTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKD
                     FKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALG
                     NTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLA
                     ELKNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEA
                     DKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRAL
                     REYNEMVDDYNSLR"
BASE COUNT        16025 a        17612 c        16984 g        15396 t
ORIGIN      
        1 gccgcaataa tgtcggatgc gatacttgcg catcttatcc gacctacacc tttggtgtta
       61 cttggggcga ttttttaaca tttccataag ttacgcttat ttaaagcgtc gtgaatttaa
      121 tgacgtaaat tcctgctatt tattcgtttg ctgaagcgat ttcgcagcat ttgacgtcac
      181 cgcttttacg tggctttata aaagacgacg aaaagcaaag cccgagcata ttcgcgccaa
      241 tgcgacgtga aggatacagg gctatcaaac gataagatgg ggtgtctggg gtaatatgaa
      301 cgaacaatat tccgcattgc gtagtaatgt cagtatgctc ggcaaagtgc tgggagaaac
      361 catcaaggat gcgttgggag aacacattct tgaacgcgta gaaactatcc gtaagttgtc
      421 gaaatcttca cgcgctggca atgatgctaa ccgccaggag ttgctcacca ccttacaaaa
      481 tttgtcgaac gacgagctgc tgcccgttgc gcgtgcgttt agtcagttcc tgaacctggc
      541 caacaccgcc gagcaatacc acagcatttc gccgaaaggc gaagctgcca gcaacccgga
      601 agtgatcgcc cgcaccctgc gtaaactgaa aaaccagccg gaactgagcg aagacaccat
      661 caaaaaagca gtggaatcgc tgtcgctgga actggtcctc acggctcacc caaccgaaat
      721 tacccgtcgt acactgatcc acaaaatggt ggaagtgaac gcctgtttaa aacagctcga
      781 taacaaagat atcgctgact acgaacacaa ccagctgatg cgtcgcctgc gccagttgat
      841 cgcccagtca tggcataccg atgaaatccg taagctgcgt ccaagcccgg tagatgaagc
      901 caaatggggc tttgccgtag tggaaaacag cctgtggcaa ggcgtaccaa attacctgcg
      961 cgaactgaac gaacaactgg aagagaacct cggctacaaa ctgcccgtcg aatttgttcc
     1021 ggtccgtttt acttcgtgga tgggcggcga ccgcgacggc aacccgaacg tcactgccga
     1081 tatcacccgc cacgtcctgc tactcagccg ctggaaagcc accgatttgt tcctgaaaga
     1141 tattcaggtg ctggtttctg aactgtcgat ggttgaagcg acccctgaac tgctggcgct
     1201 ggttggcgaa gaaggtgccg cagaaccgta tcgctatctg atgaaaaacc tgcgttctcg
     1261 cctgatggcg acacaggcat ggctggaagc gcgcctgaaa ggcgaagaac tgccaaaacc
     1321 agaaggcctg ctgacacaaa acgaagaact gtgggaaccg ctctacgctt gctaccagtc
     1381 acttcaggcg tgtggcatgg gtattatcgc caacggcgat ctgctcgaca ccctgcgccg
     1441 cgtgaaatgt ttcggcgtac cgctggtccg tattgatatc cgtcaggaga gcacgcgtca
     1501 taccgaagcg ctgggcgagc tgacccgcta cctcggtatc ggcgactacg aaagctggtc
     1561 agaggccgac aaacaggcgt tcctgatccg cgaactgaac tccaaacgtc cgcttctgcc
     1621 gcgcaactgg caaccaagcg ccgaaacgcg cgaagtgctc gatacctgcc aggtgattgc
     1681 cgaagcaccg caaggctcca ttgccgccta cgtgatctcg atggcgaaaa cgccgtccga
     1741 cgtactggct gtccacctgc tgctgaaaga agcgggtatc gggtttgcga tgccggttgc
     1801 tccgctgttt gaaaccctcg atgatctgaa caacgccaac gatgtcatga cccagctgct
     1861 caatattgac tggtatcgtg gcctgattca gggcaaacag atggtgatga ttggctattc
     1921 cgactcagca aaagatgcgg gagtgatggc agcttcctgg gcgcaatatc aggcacagga
     1981 tgcattaatc aaaacctgcg aaaaagcggg tattgagctg acgttgttcc acggtcgcgg
     2041 cggttccatt ggtcgcggcg gcgcacctgc tcatgcggcg ctgctgtcac aaccgccagg
     2101 aagcctgaaa ggcggcctgc gcgtaaccga acagggcgag atgatccgct ttaaatatgg
     2161 tctgccagaa atcaccgtca gcagcctgtc gctttatacc ggggcgattc tggaagccaa
     2221 cctgctgcca ccgccggagc cgaaagagag ctggcgtcgc attatggatg aactgtcagt
     2281 catctcctgc gatgtctacc gcggctacgt acgtgaaaac aaagattttg tgccttactt
     2341 ccgctccgct acgccggaac aagaactggg caaactgccg ttgggttcac gtccggcgaa
     2401 acgtcgccca accggcggcg tcgagtcact acgcgccatt ccgtggatct tcgcctggac
     2461 gcaaaaccgt ctgatgctcc ccgcctggct gggtgcaggt acggcgctgc aaaaagtggt
     2521 cgaagacggc aaacagagcg agctggaggc tatgtgccgc gattggccat tcttctcgac
     2581 gcgtctcggc atgctggaga tggtcttcgc caaagcagac ctgtggctgg cggaatacta
     2641 tgaccaacgc ctggtagaca aagcactgtg gccgttaggt aaagagttac gcaacctgca
     2701 agaagaagac atcaaagtgg tgctggcgat tgccaacgat tcccatctga tggccgatct
     2761 gccgtggatt gcagagtcta ttcagctacg gaatatttac accgacccgc tgaacgtatt
     2821 gcaggccgag ttgctgcacc gctcccgcca ggcagaaaaa gaaggccagg aaccggatcc
     2881 tcgcgtcgaa caagcgttaa tggtcactat tgccgggatt gcggcaggta tgcgtaatac
     2941 cggctaatct tcctcttctg caaaccctcg tgcttttgcg cgagggtttt ctgaaatact
     3001 tctgttctaa caccctcgtt ttcaatatat ttctgtctgc attttattca aattctgaat
     3061 ataccttcag atatccttaa ggaattgtcg ttacattcgg cgatattttt tcaagacagg
     3121 ttcttactat gcattccaca gaagtccagg ctaaacctct ttttagctgg aaagccctgg
     3181 gttgggcact gctctacttt tggtttttct ctactctgct acaggccatt atttacatca
     3241 gtggttatag tggcactaac ggcattcgcg actcgctgtt attcagttcg ctgtggttga
     3301 tcccggtatt cctctttccg aagcggatta aaattattgc cgcagtaatc ggcgtggtgc
     3361 tatgggcggc ctctctggcg gcgctgtgct actacgtcat ctacggtcag gagttctcgc
     3421 agagcgttct gtttgtgatg ttcgaaacca acaccaacga agccagcgag tatttaagcc
     3481 agtatttcag cctgaaaatt gtgcttatcg cgctggccta tacggcggtg gcagttctgc
     3541 tgtggacacg cctgcgcccg gtctatattc caaagccgtg gcgttatgtt gtctcttttg
     3601 ccctgcttta tggcttgatt ctgcatccga tcgccatgaa tacgtttatc aaaaacaagc
     3661 cgtttgagaa aacgttggat aacctggcct cgcgtatgga gcctgccgca ccgtggcaat
     3721 tcctgaccgg ctattatcag tatcgtcagc aactaaactc gctaacaaag ttactgaatg
     3781 aaaataatgc cttgccgcca ctggctaatt tcaaagatga atcgggtaac gaaccgcgca
     3841 ctttagtgct ggtgattggc gagtcgaccc agcgcggacg catgagtctg tacggttatc
     3901 cgcgtgaaac cacgccggag ctggatgcgc tgcataaaac cgatccgaat ctgaccgtgt
     3961 ttaataacgt agttacgtct cgtccgtaca ccattgaaat cctgcaacag gcgctgacct
     4021 ttgccaatga aaagaacccg gatctgtatc tgacgcagcc gtcgctgatg aacatgatga
     4081 aacaggcggg ttataaaacc ttctggatca ccaaccagca gacgatgacc gcccgcaata
     4141 ccatgctgac ggtattttcg cgccagaccg acaagcagta ctacatgaac cagcaacgta
     4201 cgcagagtgc gcgtgaatac gacaccaacg tgctgaagcc gttccaggaa gtgctgaatg
     4261 accctgcgcc gaagaaactg atcattgttc atctgctggg tacgcatatc aaatacaaat
     4321 accgctaccc ggaaaatcag ggcaagtttg atggcaatac cgatcatgtt ccgccgggat
     4381 taaacgcgga agagctggag tcatataacg attatgacaa cgctaacctg tataacgatc
     4441 atgtggttgc cagcctgatt aaagacttta aagcagcaaa cccgaacggt ttcctggttt
     4501 atttctctga ccacggtgaa gaggtttacg acacgccgcc gcacaaaact caggggcgta
     4561 atgaggacaa cccgacgcgt catatgtaca ccattccgtt cctgctgtgg acgtcagaaa
     4621 aatggcaagc gactcatccc cgtgatttct cgcaggatgt tgatcgtaaa tacagcctgg
     4681 cggaactgat ccacacctgg tcagatttgg cgggcttatc ttacgacggt tacgatccaa
     4741 cccgttcagt ggtgaatccg cagttcaaag aaactacccg ctggattggt aacccgtata
     4801 agaaaaacgc actgatcgat tacgacacac tgccctatgg cgatcaggtg ggtaatcagt
     4861 aattatcagc caggcgctat ttgccggatg cgacgcttga cgcgtcttat ccggcctaca
     4921 gatcgcggcg gtaggctgga taaggcgttt acgccacatc cggcaataca actgccgcaa
     4981 tgtttacatc gaactcccct caggccacac ttttcaggtg gcctttttta ataatgccag
     5041 ttactgtttt cccgtctaca tacctaaggg aataacatgt atcacgacgt cagctatctg
     5101 ctctcccgcc tgatcaacgg cccgctgtcc ctgcgccaga tttactttgc cagcagtaac
     5161 ggcccggtgc ccgatcttgc gtatcaggtc gattttccac ggctggaaat tgtgctggaa
     5221 ggtgagtttg ttgataccgg cgctggagca acgttagttc ccggcgatgt gctgtacgtt
     5281 cctgctggtg gctggaattt tccacaatgg caagcccccg ctactacctt tagcgtgctg
     5341 tttggcaaac agcaactcgg cttcagcgtc gtgcaatggg atggcaaaca atatcaaaat
     5401 ctggcgaagc aacacgtcgc ccgacgcggc ccacgcatag gttcttttct gctacaaacg
     5461 ctcaatgaaa tgcaaatgca gccgcaggag cagcaaacgg caaggcttat cgtcgccagc
     5521 ctgcttagcc actgccgcga tttgcttggc agccaaatcc agaccgcctc acgcagccag
     5581 gcactatttg aagctattcg cgattatatc gacgaacgct atgcctccgc gcttacccgc
     5641 gaatctgttg cacaggcgtt ttatatttcg ccaaattacc tctcgcacct gtttcaaaaa
     5701 acgggggcca ttggttttaa cgaatacctg aatcacacgc gactggagca cgctaagacg
     5761 ttattgaaag gctatgacct gaaggtaaaa gaagtggcgc acgcctgcgg ctttgtcgac
     5821 agcaactact tctgccgtct gtttcgtaaa aacaccgaac gctcgccgtc ggaatatcgc
     5881 cggcagtacc acagccaact gacagaaaaa ccgactactc cagaataaga tgggtttgct
     5941 gcggcgcaga aagcactttg cgcaccgcgc tcattacccg ctgcggctca cgcaggaatg
     6001 cgtagatgct gcattgcaca tagcggcaat gttcaaatcg ctcggcaccg gcaagctcga
     6061 tatccgtaat caacagagca acatccgccc ggcggatatc ctcatccgct aaacgattct
     6121 ccgttcccag cgcgccctga gtttcaatgc tgactcccca cttctctaac aggcacaact
     6181 tttccagccg ttccgccgcc atataagtat gcgccacgcc gcttacgcag gcggttactg
     6241 ccaccaggta tgccatttta acctcccacg gtaacctgaa gtccggcccg ttctgccatt
     6301 tcgcgcatcg ttgccacatc agctgacgac ggcgcaggca cctctttcat cgaccatgtt
     6361 ttccccagca ggcggtattt cggttcgccg tactgatgaa acggtaacag atggatctgc
     6421 ctgatattca gcgggatcag tacatccagc gcctgctgca tattctcccg gctgagcgtg
     6481 aaaccaggga tcagcggtaa acgcgggatc acgttgacgc cctcactcac cagcaaacgc
     6541 agattctcca gcacgcgtgg caggttcatc ttcaccacat cccgcgcctg agtcgcgtcc
     6601 ataattttta aatcgaacaa cacttcatcg cacaatttcg ccagcggtaa tagcttgctg
     6661 gctggtgcgt ctccggcagt ttcaatggcg catgacacac cccacagccg cagtcgctgt
     6721 aaaaaacggg tagcaaactc cgcctgcatt aacacttcgc cgccagaaag cgtgacgccg
     6781 ccgccggacg tgcgaaagaa aatgtcatct ttcatcactt cccgttccag agcgtcaagg
     6841 ctgatatcgc gaccaatccg ttcaaacgcc ccggaggggc attcatccgc atcacgcaaa
     6901 catttcgcac agtgcagaca tttcgcctct ctgcgtaccg tctggatttt gccggagatc
     6961 gactccggat tagcacacca cgggcaaaga tgcggacagc ctttaaaaaa gaccaccgta
     7021 cgaatgccct cgccgtcatt cagtgaataa cgctgaatgt tgaaaatgcg cgccacatca
     7081 tcgcggcgcg tttccacaac gttacagctg atgcgctgtc cggcggatga tgtcatcctg
     7141 gatctccttc gacagttcga caaagaaggc gctgtatccg gcaacgcgca ccaccagccc
     7201 ggcataatct tgtgggcgct gttgcgcttc ccgcaacgtg tcggcgttca ccacgttaaa
     7261 ctgaatatgt tgtaacttaa gctgggtaaa cgcccgtaag aagtcggcca gtttgcgtaa
     7321 tcctgcttca ccttccaggg tcgccggagt gaatttcacg ttcagcaacg taccgttaga
     7381 cagcagcgtg ttatcgagct tactgactga cttcagtacc gctgttggcc cttgtgcgtc
     7441 ctggcccagc atgggtgaca agccgccgtc tgccagctgt tctccggcaa aacgaccgtc
     7501 tggcgtcgcg ccaaccaccg atcccaacgg gacgtgagca gaaacggtat acgatcccgg
     7561 cgtgaagtag ccgccgcgcg ggttctggta tttttccact tctttgcagt agtggcgcaa
     7621 cagttcggcg ctgatgttat ccacctcgtc gatatcgtta ccgtatttct cgaagcggtt
     7681 aattaagcga gcgcggactt tttcgccttc tggcgttgcg aagttggctt ttaataccga
     7741 cagcaattcg tcaaaactta aacgctgttg ctcaaaaacc atccctttca acgcatggag
     7801 agaatcgctc aggttggcga taccgatccc ctgtacgccg gagaagttat aacgcgcgcc
     7861 gccatcggta atatcgcggc ctttttccag acaatcgctg ataaacgatg agagcagcgg
     7921 tacaggtgcc cagtcgcgat ggccgatatc acaaatattg ctgccctcaa ccatcagggt
     7981 gatgtagtgg ctgatcttgg cgcggatctg ctccagtaaa ccttcataag tcagcgcagc
     8041 attgccttca ttttcatgca ggcagatttc catcactttc agcagattaa acatcgcgat
     8101 gtcatgcaag ccgtaggttc tgccgggaat agataattcc acacagccca ctacggaata
     8161 gtcgcgcgca tcttccagcg aaacgccacg gttgaggaac gctggcacca ccacttcatc
     8221 gttaaagatt tgcggaatac cggtgccgaa gcgaatggtt tcggcggttt tcatcaggaa
     8281 cggcgtgtcg atcaaggcgt tagtgcgcac gccgaggttc ggttgcggta attgcacgct
     8341 ttgataggcg tcaaggcaaa ggaacgaaag cacgttcacc gcgctacgtc cgttctcggt
     8401 taacccgccg agcagtgcgg tatagccggt cgggaaaccg gcgaaataac gcgcgctact
     8461 ggtggagcgc aacagcacga tgtcgttgca tttcacccat aaagattcga gcagttcttt
     8521 caggaacgcc gcatcttcgc cctgggttaa tgatgtctga tagaacggca acatatactg
     8581 gtcgaagcgc cccaacgata gcgaactggc gttggattcg tattgcagaa tgatgttcat
     8641 gtaccagaat aactggcacg cctgccagaa cgtctgcggc ttatgttgcg cgttatggcg
     8701 ggagatctct gcaatagtca gcagctcttc gcgacgctgg gcatctgtgc agtttgccgc
     8761 catcgtttcc gccagttcgg cgtaacgcaa aatgtgtttc tgcgaggctt ccagcagtaa
     8821 caacgcggcc tgataaaagt gattctccgg ctgttgctga caatgttgct gcatctgtgc
     8881 caccagctca cccagcccgt gattcaacag gcgtgggtaa tcaataataa tgtgcccctg
     8941 gcctttatcc gtctggttga tgctgaaaat ctgcgtgttg gtcgcggctt ttacttcatc
     9001 tgtcatctgc ccgttgatga aatctttcat cgaacgtttt tcccagtacg ggaacaactc
     9061 ttcacggtag atacgtttat cttcttcgct gatagcaaag cggtcctgcg gacgcgtcgg
     9121 gaattgatcc agctctttca gcagccagta agggtccatt tccggcgaca taatcccggc
     9181 gcgcggtttt acggtgcggt taccggcaat cagttcttca tcacgaatcg aaatttcaac
     9241 atgttcaagg atatacgctg ttgctttcgc ccggcgcaat atcaccggtt cgccttcggt
     9301 ttgccgatgg ctggcggtat aaagcagcgc ccgctccagc gagatttcac gggtattggc
     9361 aaacagtgca gttttgaggc gagagatacg attcgtcatg aggaactcct gtgggaaagc
     9421 gaacccgccc ttgcgagcgg gagaagaact taagcggttt gcgccaggtg agcttcaatt
     9481 ttgctcataa tggcgtcagc acgttttact gcatcgctga tgttgacgcg cacgatggtc
     9541 ttgccagcaa aacgctcttc aaacttgatg ccgatatctt tggtcagaat caccatatca
     9601 gcgctcgcca catcttccgc agtcagttca ttttccagac caattgaccc ctgggtttct
     9661 actttcactt cccagccttt cgctttcgcg gcactttcca gcgcctctgc tgccatatag
     9721 gtatgggcaa cacctgaagg acatgcggtt actgcaataa ttttcgtcat ggctcgttcc
     9781 tcaattaatt aatttcaaaa tccaaatcca ggtcgtcttc tttttcatca gtcgaagacc
     9841 catttttacg cgccagactt ttcagcacgt tcacacaaac agccgtcacc actgctccga
     9901 cagccactgc tgcgatataa cccagcttgc cttcaaccac cggcagcaca atcagaccac
     9961 cccagcctgc atagcactgc gcaccgacca gcgccgcagt tactgcacca caaactgaac
    10021 cgaccatgat ggaaggaatg acacgcagcg gatcggcagc ggcgaaagga atcgcccctt
    10081 cagtaacgcc aacacagccc atcaccagtg ccgctttgcc ggtttcgcgc tcttctgcgg
    10141 agaaattttt acgaccaatc agcgtcgcca gccccatccc cagcggcggg atacaaatac
    10201 ccacggcagc gatggccacc acggtataaa caccctgagc aacgcaaatc agcatgaagg
    10261 cataggccac tttgttaacc ggaccgccca tatcgaacgc cagcatcaga cccatgatca
    10321 ccgccagcat aacaatgctg ccctgctgca tcccctgaag ccactgagtc aggctgttgg
    10381 tcaacgcccc tacaggctcg cccaagcccc acatcatgat gcctgcggta atcagtgtgc
    10441 cgacgatagg aataatgaag ataggcatca ccgagcgcag aactttatgc accggaattt
    10501 tcttcaggta atgcaccacg atgccgccga taatcccggc gatcagtgca ccaaagaagc
    10561 ccgcaccaaa gctgttacca acccaggcac cgatagcgca cggagccagc gcagaacgct
    10621 ctgcaatgga ataaccgatg tacgctgcga ggaaaggcac catcagcgtc aagcccgcaa
    10681 cgccgatatc aaacagtttt ttcagatttg gatcggctac ggcatccggc actgcgcctt
    10741 tgccatacaa catgacggaa accgccagca aaataccgcc cgataccacg aagggaatca
    10801 tgtgtgaaac gcccgtcatt aaatgctgac gggtattttt caggatctgc accaactcat
    10861 tcataagctg ctcccgggct tgcgaaagcc catgtccata tttttgatgg aggaaacaat
    10921 aggcaatcgc aggcggagca aacagtggat aaaaaggcca tttactggac ttttgtaata
    10981 tcagtacaaa aatgcgatcc gcctcataac ttgcgataaa ccggaagcat gccgcaaaac
    11041 agcgtcttgt gagacgcgtc gcaaattgac ggttacatta catttgtcca gcttttggca
    11101 gatttgtcac atggcaggcc gttgccgtgc gctcttatcc tgtaaacaga aaaccgtacc
    11161 gggagagagg caatggccct gattgtggaa tttatttgtg agctacctaa cggcgtacat
    11221 gcgcgtccgg caagccacgt tgaaacgctg tgtaatactt tttcatcaca aattgagtgg
    11281 cataacctgc gcactgaccg caagggcaac gccaaaagcg cccttgcgct gattggcacc
    11341 gatacgctgg cgggcgataa ctgccagtta ctgatttccg gggccgacga acaggaagcg
    11401 caccagcgtt taagccaatg gctgcgcgat gaattccccc actgcgatgc gccgctggcg
    11461 gaagttaaat ctgacgaact ggaaccactg ccggtttcac tgaccaatct gaatccgcaa
    11521 attatccgcg cccgcaccgt gtgcagcggt agtgcaggcg gcattctgac gccgatctct
    11581 tctttagatc tcaatgcgct gggtaatctt cccgcagcca aaggcgttga cgccgagcaa
    11641 tccgcactgg aaaacggcct gacgctggta ctgaaaaaca ttgagtttcg tctgctggat
    11701 agcgacggtg ctaccagcgc gattctggaa gctcaccgat ccctggctgg cgatacttcc
    11761 ctgcgcgaac atttactggc aggcgtcagc gccggattaa gctgcgccga agcaattgtt
    11821 gccagcgcga atcacttttg cgaagagttt tcccgttcca gcagcagcta cctgcaagaa
    11881 cgtgccctgg acgtacgcga cgtctgcttc cagttactcc agcaaatcta cggtgagcaa
    11941 cgcttcccgg caccgggcaa actgacgcag cccgccattt gtatggctga tgaactgacc
    12001 cccagccagt tcctcgaact ggataaaaat cacctcaaag gattgttgct caaaagcggc
    12061 ggcaccacct cacatacggt gatccttgcc cgttcgttca acattccaac gctggttggt
    12121 gtggatattg atgcccttac tccgtggcag caacaaacga tttatatcga cggcaacgcc
    12181 ggggcgattg tggttgagcc aggggaagcc gtagctcgtt attatcagca agaagcccgc
    12241 gtacaggacg ccctgcgtga gcaacagcgt gtctggctga cccaacaagc ccgtaccgct
    12301 gacggtatcc gcattgaaat tgccgctaac atcgctcact ccgtggaagc gcaggccgca
    12361 ttcggcaatg gtgcggaagg cgttggtttg ttccgcactg aaatgctcta tatggatcgc
    12421 accagcgcac cgggcgaaag cgagttgtac aacatttttt gtcaggcgct ggaatccgcc
    12481 aacggacgca gcattattgt gcgcactatg gacattggcg gcgacaaacc cgttgattat
    12541 ctgaacattc ccgcagaggc aaacccgttc ctcggttatc gcgccgtgcg tatttatgaa
    12601 gagtacgcgt cgttgtttac cacgcagcta cggtcgatcc tccgcgcctc cgctcacggc
    12661 agcctgaaaa tcatgatccc gatgatctcc tcaatggaag agatcttatg ggtgaaagaa
    12721 aaactggcgg aagccaaaca gcaactacgt aacgaacaca ttccgtttga tgagaaaatc
    12781 cagctcggca tcatgctgga agtgccgtcg gtgatgttca tcatcgatca atgctgcgaa
    12841 gagattgatt tctttagtat tggtagtaat gacctgacgc agtatctgct ggcggtggat
    12901 cgcgataacg ctaaggttac tcgtcactac aacagcctga atccggcatt cttgcgggcg
    12961 ctcgattacg ccgtgcaagc ggtgcatcgc cagggcaaat ggattggtct gtgcggtgag
    13021 ctgggagcga aaggttccgt gctgccgttg ctggtcggct tagggctgga tgaactcagc
    13081 atgagcgcac catcaattcc ggcggcgaaa gctcggatgg cgcaacttga tagccgtgag
    13141 tgccgcaagt tgctcaacca ggcaatggcc tgccgtactt cgctggaagt ggaacacctg
    13201 ctggcgcaat tccgcatgac ccaacaagac gcaccgctgg tcaccgccga gtgcatcaca
    13261 ctggaaagcg actggcgcag caaagaagaa gtgctcaaag gcatgaccga taacctgctg
    13321 ctggcgggcc gctgccgcta tccgcgtaaa ctggaagccg acttgtgggc gcgcgaggcc
    13381 gttttctcta ccggtctggg ctttagtttt gccattccac acagcaaatc agaacacatt
    13441 gagcaatcca ccatcagcgt ggcgcgtctg caagcgccgg tgcgctgggg cgatgatgaa
    13501 gcgcaattca tcattatgtt aaccctgaac aaacacgctg cgggcgatca gcatatgcgc
    13561 attttctcgc gcctcgctcg ccgcatcatg cacgaagaat tccgtaacgc gctggttaac
    13621 gccgcctctg ccgacgctat cgccagcctg ctgcaacatg aactggaact gtaaaaggaa
    13681 acatcatgga actgtatctg gacaccgcta acgtcgcaga agtcgaacgt ctggcacgca
    13741 tattccccat tgccggggtg acaactaacc cgagcattat cgctgccagc aaggagtcca
    13801 tatgggaagt gctgccgcgt ctgcaaaaag cgattggtga tgagggcatt ctgtttgctc
    13861 agaccatgag ccgcgacgcg caggggatgg tggaagaagc gaagcgcctg cgcgacgcta
    13921 ttccgggtat tgtggtgaaa atcccggtga cttccgaagg tctggcagca attaaaatac
    13981 tgaaaaaaga gggtattact acacttggca ctgctgtata tagcgccgca caagggttat
    14041 tagccgcact ggcaggggca aaatacgttg ctccgtatgt taaccgcgta gatgcccagg
    14101 gcggagacgg cattcgtacg gttcaggagc tgcaaacgct gttagaaatg cacgcgccag
    14161 aaagcatggt gctggcagcc agctttaaaa cgccgcgtca ggcgctggac tgtttactgg
    14221 caggatgtga atccatcacc ctgcccttag atgtagcgca acaaatgctc aacacccctg
    14281 cggtagagtc agctatagag aagttcgaac acgactggaa tgccgcattt ggcactactc
    14341 atctctaaag gagcaattat ggaccgcatt attcaatcac cgggtaaata catccagggc
    14401 gctgatgtga ttaatcgtct gggcgaatac ctgaagccgc tggcagaacg ctggttagtg
    14461 gtgggtgaca aatttgtttt aggttttgct caatccactg tcgagaaaag ctttaaagat
    14521 gctggactgg tagtagaaat tgcgccgttt ggcggtgaat gttcgcaaaa tgagatcgac
    14581 cgtctgcgtg gcatcgcgga gactgcgcag tgtggcgcaa ttctcggtat cggtggcgga
    14641 aaaaccctcg atactgccaa agcactggca catttcatgg gtgttccggt agcgatcgca
    14701 ccgactatcg cctctaccga tgcaccgtgc agcgcattgt ctgttatcta caccgatgag
    14761 ggtgagtttg accgctatct gctgttgcca aataacccga atatggtcat tgtcgacacc
    14821 aaaatcgtcg ctggcgcacc tgcacgtctg ttagcggcgg gtatcggcga tgcgctggca
    14881 acctggtttg aagcgcgagc ctgctctcgt agcggcgcga ccaccatggc gggcggcaag
    14941 tgcacccagg ctgcgctggc actggctgaa ctgtgctaca acaccctgct ggaagaaggc
    15001 gaaaaagcga tgcttgctgc cgaacagcat gtagtgactc cggcgctgga gcgcgtgatt
    15061 gaagcgaaca cctatttgag cggtgttggt tttgaaagtg gtggtctggc tgcggcgcac
    15121 gcagtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    15181 gtggcattcg gtacgctgac gcagctggtt ctggaaaatg cgccggtgga ggaaatcgaa
    15241 accgtagctg cccttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    15301 aaagaagatg tcccggcgaa aatgcgaatt gtggcagaag cggcatgtgc agaaggtgaa
    15361 accattcaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc tctgctggta
    15421 gccgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    15481 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    15541 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    15601 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggaatttt ttatccgctt
    15661 ctggacattt tctctacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    15721 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    15781 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc aggccaatac
    15841 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    15901 ggcgtatgtg cagcttactt atcctggcgg tgatgttccg caagaacgcg gtgtatgttc
    15961 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    16021 agatatggcg aagaattttg ctgagtaccc gcaaaaatgg aagttaaagc gcccggacag
    16081 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc atgataaaac
    16141 acgccccacc agcaagaacc ccagtgacta tcaagcgggc gatattgtct cctggcgact
    16201 ggataacggg ctggcacata ttggggtggt ttcagatggc ttcgcccgcg acggtacgcc
    16261 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttaactggcg
    16321 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    16381 tcagatcctt tttacactga cggcttcttt tttcttacgg ctcaccagcg ccagtgccag
    16441 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcgccga gcagccacca
    16501 ggaaaagagt acgccgcaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    16561 caagtttttc aatacaaaca accataagct ccacgccagt gccgtcgcca gaatcgcact
    16621 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    16681 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgacaataa
    16741 atccacgcgc ggatgacggg catacagacg tttagcaaca atcgcgctcg ccccccaact
    16801 gacgccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    16861 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    16921 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    16981 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    17041 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggtgtcg ggcgcattcc
    17101 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    17161 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    17221 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    17281 agacatgaga gatccagtgt gtagttttcg ttcgcccact atacgctggt aaagaacgtt
    17341 cagcgactgc cagcaagcag ccgctgaacg gggtcagatt acagcaggtc gaaacggtcg
    17401 aggttcatca ctttcaccca tgccgccacg aagtctttaa caaacttctc gtgggcatcg
    17461 ctactggcgt aaacttccgc caccgcacgc aggacggagt tagaaccaaa caccagatcc
    17521 gcacggctgg ccgtaaattt cacttcgccg gtttcacggt cacggccttc gaacagctct
    17581 ttcgattcgt cggtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    17641 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    17701 cccagtacac gcatgccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    17761 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    17821 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    17881 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    17941 gctttctcaa caccaaccac accagccagc actatgatat ccgccagcga ggctttacca
    18001 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    18061 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    18121 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    18181 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    18241 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    18301 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    18361 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    18421 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc aaacggatcc
    18481 gggataattt ccggtgcgtc taccgcttcg aactggattg cgccagccgg gctgcgggtc
    18541 tgtacccact catacttgaa caggttctcg aagaaatagt tgctccactg ggtcggcgtc
    18601 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    18661 gtgctcgccc aacctaaacc ttgttcttca atcggtgcag cttctggatc aggacctaca
    18721 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    18781 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    18841 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag acccatctcg
    18901 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    18961 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    19021 ccgaaggtac ggaagccgga gttttctagc gccacgttac ccgcgaggat aaacaggtcg
    19081 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    19141 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    19201 gcgccaccgc gtccatcgat tgaacggtaa gtccccgcgc cgtgccaggc catacgaata
    19261 aacagaccgg cgtaactgcc ccagtcggct ggccaccacg gttgagattc tgtcaacagg
    19321 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    19381 aagtcctcac ccagtgggtt agaacgatta gaatgttggt ttaacaggtc aacacgaagt
    19441 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cactctggtc gtgaccgccc
    19501 tgatgaaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    19561 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    19621 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    19681 atcaaattga tcttaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    19741 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgataacct
    19801 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    19861 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaaccccca gcgtatggca
    19921 aatcgcgtaa ctcatttcag cacggttaag cgtatagaag tggaaatctt tcactccttc
    19981 acggcttaaa atcttcacca tatccatggc aatattcgcg ccaaccagtt tgcgggtttc
    20041 ggcatcatca tccagaccgt cgaacatttg cgccatccac gccggaatac gcacgttggt
    20101 catatcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaattc ccggaataat
    20161 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    20221 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    20281 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggagat
    20341 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    20401 accacttccc ggcggcagat cgccacgcag cgccacgata tgacgaatac cgttattcca
    20461 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    20521 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    20581 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    20641 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    20701 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    20761 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    20821 cacatctatc cgtttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    20881 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    20941 aagaaaaaga aaatccggta tgcaaagcaa accggactgc aaatataaac taaccatgta
    21001 cttattgctt cttgccggat tcgtcgtaaa cgcttaatcc ggcctaatcc tatgataatt
    21061 caatatattt cattagaaac cataaacccg aaaacatgag taccgggcat tattaaattt
    21121 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    21181 gtcgcgccca gcgccaggtc cgcggatcac cagagggtta tcgcgatacc agcggctttc
    21241 gatggcaaag acgttatcgc acggcagcag tgatgccaac ggatgatctt cacgcaccgc
    21301 ttccacgcct acacgcgctt taccgttggc atcgaaacgc gccacgtagc gcagcaccag
    21361 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttcagtt catcgccatt
    21421 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    21481 tacctgatcc ggttcgatgt tgtaacctgc ttcacgcgcc agaatcacca gcttgcgcat
    21541 cacgtctttg ccagagagat cgtcacgcgg gtcaggttcg gttaagccct gctgccacgc
    21601 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    21661 cgtgccggag aagatcccgc tgatcgacaa aatagtatcg ccgctgtcga tcagatcgcg
    21721 cacggtgtgg ttgatcggca agcccgcacc gacggtggca ttgtacagcc agtgacgccc
    21781 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgctgg ctcccgccag
    21841 tttgttggcg ctgataacgt ggaaaccgtg gctggcgaaa tcaagatact gatcagcaag
    21901 ctgctggctg gcggtaacgt ccagcaccac taaatcatca tacggatggg cgcgcatcca
    21961 caggaacaac gactcttcat cctgctcaac cgcttcatcg ttgaagaagg ctaacgcgcg
    22021 gctggcgtcc agcccgtcat agctcaacag gctgcggcgg ctgtccacca cacctgccag
    22081 cacaaactca aagccggtac gtgccgaaag cgtgctctgc tcacgggcga acagttccag
    22141 ccaacgggaa ccgatattgc ccttaccgaa caataccagg ccgatgcgtt tttctgcgcg
    22201 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    22261 caccaggctg atgccgtcat cggactgcca ggtaaattcg accggctggc ctttcagttg
    22321 ctgccagaag cggtggcaat gcagcgggtt acgggtgacg cctgcaccga ccatcgccac
    22381 cagcgccagc ccctgacgca ggcgcagttc gccaggtaat cccgcttcgt cgaggatttt
    22441 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc agcaactggc gatcgttatg
    22501 tacgccaacc gccagcgggc gtacctgcgc gcgtttcagg atttggtcga tctctttatg
    22561 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    22621 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    22681 ttgatccggc gtgtagctac agcgcagttg caggtcgatt tcgctgccag aaaccggctg
    22741 taaagtacgg gcgtgaagaa cgggagccgc caggcgcgcc agttcgctgg cctcatccag
    22801 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    22861 ggcgacgtcg ctccagatgg ttacgcgaga aacacccgcc agcgcaccga tttgtgtcgc
    22921 ggaatagtcg gaaccgttac gccccagcag caccgtttca ccggcgttgt tgcggctgat
    22981 aaatccggtc accaccagac gtttgcccgg atgttgcacc agcagctgtt gcagcaacgg
    23041 gtaagaaagc ccttcatcaa cctgcggttg tgcggcgcgt tcagcgcgta aaaactcgcg
    23101 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcagacgtgc
    23161 cgaccatact tccccgtggc ccaccacttc cgcatacact gcgtcgttaa taccgctgtc
    23221 gagcagcgcc gccaggcgct caaggtcgct gacaaaagcg ctaatgaggc tatcggcttc
    23281 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    23341 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    23401 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagtact ccgccataat
    23461 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    23521 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    23581 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    23641 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    23701 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    23761 aacaacgaca gcccgcccag gaaacgacgc agcgtctgct catcgccatc cagttcaaaa
    23821 ctcaacattg cgccaaagcc tttttgctgg cgcgcggcaa tttcatgccc ctgattttcc
    23881 ggcaacgacg ggtgatacag ttttttcacc aacggctggg tttgcaggta tttcacaatc
    23941 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccacgtagc
    24001 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggccagt
    24061 tcagtgacaa cgtccgggtc tttagcaatc accacgccgg ccactacgtc tgagtgaccg
    24121 ttcagatatt tcgtgcatga atgcaacacc agatcggcac ctaatgccag cggattttgt
    24181 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    24241 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    24301 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    24361 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    24421 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    24481 tgaatcgcgg acatgccggt attagtaagt actgcaccag caccaccttc cagttctgcc
    24541 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    24601 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatcggtgg gacaacgcaa
    24661 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    24721 atgtgatgaa gttccctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    24781 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    24841 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    24901 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacatca actgtgtggt
    24961 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    25021 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    25081 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    25141 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    25201 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    25261 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagca
    25321 aaaaagagcg gcgcggagtg gaatcgcctg atgcgctacg cttatcaggc ctacgtcata
    25381 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    25441 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    25501 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    25561 cacaaaccgg cgacatcatc tttcagatct ctcgctcatc gcaaagtaaa gcgatccaac
    25621 tggcgaccca taccgattat agccacaccg gtatgctggt gatacgcaac aaaaagccct
    25681 acgtttttga agcagtcggc ccggtgaaat acaccccgct caagcagtgg atcgcccatg
    25741 gtgaaaaggg caaatacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    25801 aaaaactggc gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    25861 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    25921 gaatgcgcgt gggcgagcag cagaagttga aagagttcga cctgagcagc ccacaagttc
    25981 aggcaaagct caaagaacgt tacggcaaga atattccgct tgaggaaacg gtggtctcgc
    26041 cacaagccgt tttcgatgcg ccacaactca ccacggtcgc caaagaatgg ccgctgtttt
    26101 cgtggtaata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    26161 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    26221 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    26281 aggttcaggt catgaccaac ggtggagcgg attttcatta cgttaccgca agagcagcta
    26341 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    26401 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    26461 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    26521 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    26581 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    26641 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    26701 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcaatgag
    26761 ctaaaagcgg tagtcgaagt gctggatagt gagccggtgt ttactcactc cgtctggcga
    26821 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    26881 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    26941 gccactgaac aaggccaggc ggtggatctg aacagcctga aacgctcccc caagcaacaa
    27001 caggcgctgg cggcgttacg gcaaggcaaa atctggcgcg accaggtcgc cacgctcgaa
    27061 tttaatgatg ccgcgttgca ggcgctacgc aaaaaaggtc tgtgtgattt agcaagtgaa
    27121 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    27181 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    27241 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag cgtactggaa
    27301 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    27361 caaactatcg cccgttttcg tgaacgtttt aatgcccccg tggaagttct gcattccggc
    27421 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggtga ggcggcgatt
    27481 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    27541 gatgaagagc acgacagctc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    27601 ctggcggtgt atcgtgcgca cagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    27661 gcgctggaaa cgttatgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacccgt
    27721 cgggcaggga atgcgcgtcc ggcaattcaa catgtgctgg atttaaaagg tcagaaggtg
    27781 caggcaggtc tggctccggc gttaatcact cgtatgcgcc agcatttaca ggctgataac
    27841 caggtcattc tctttcttaa ccgccgtggc tttgcgcctg cactgctgtg ccacgactgt
    27901 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcagcac
    27961 catctgcgct gccaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    28021 ggttccacgc acctggtccc cgtggggctg ggcaccgaac agcttgaaca gacgctcgcg
    28081 ccgttgttcc ccggcgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    28141 gcgctggaac agcaactggc agaagtacat cgcggcggcg cgcggatttt gattggtaca
    28201 caaatgctgg cgaaaggtca ccatttcccg gatgtgacgc tggttgcatt actggacgtg
    28261 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    28321 caggtcgccg gtcgtgccgg gcgtgcgggt aaacagggcg aagtggtgct gcaaacgcac
    28381 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc ctttgccgaa
    28441 caggcgctgg ctgagcggcg aatgatgcag ctaccgccgt ggaccagcca tgtgattgtg
    28501 cgtgcggaag atcataacaa tcagcacgcg ccattgttcc tgcaacaact gcgtaatctg
    28561 atcctctcca gcccactggc agacgagaaa ctgtgggttc tcggtccggt tccggctctg
    28621 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    28681 cgcttgcaac acatcattaa cggtacgctg gcgctcatca atacaatacc ggattcccgt
    28741 aaggtgaaat gggtgctgga tgttgatccg attgagggtt aaaccgctca cgatgcgagg
    28801 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    28861 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    28921 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    28981 ttgccctcaa ggcaaaagtc tctacagcga ccgtctcccg agcattaatg aatcccgata
    29041 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    29101 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    29161 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    29221 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    29281 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gttgctgggt tcaaggctgc
    29341 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    29401 ttgcaccgga gctggagctg cctacagttc atatcgacaa tctgaccgcc gcatttgatg
    29461 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    29521 agatgccgct gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggca
    29581 ttatggttga tccgcaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    29641 cgatgcagca gctgcttgat cttccacaac cgcctactgc tgtcttctgc catagcgatg
    29701 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    29761 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    29821 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    29881 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    29941 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    30001 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    30061 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    30121 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    30181 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    30241 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    30301 tggcgctaca ttaaagagct ggaaagtcgc cagccgggag tgcgtgcgcc cacagaacct
    30361 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    30421 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaagt tccgtggaat
    30481 gaacagacgc cggaacagcg tcagcaaacg ctacagcgcc aacgtcaggc gcagcaatta
    30541 gcggaacagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    30601 acgcgtacgt cgcaagccgc accggtacag gcgcaaccgc gccagtctaa accggcttct
    30661 tctcagcagc cgtaccagga tctgctgcaa actcctgcgc acacgactgc gcagtcgaag
    30721 ccgcagcaag ctgcgccagt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    30781 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    30841 acggtacgtg ctcagctggc gttcgaaggc tttgactcga aaatcaccac caacaatggc
    30901 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    30961 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    31021 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    31081 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    31141 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    31201 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    31261 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    31321 tggtcaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    31381 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    31441 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    31501 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    31561 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    31621 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    31681 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    31741 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    31801 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    31861 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa attcaccgaa gtgggctacg
    31921 tcggtaagga agtagattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    31981 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    32041 tcgacgtgct gatcccacct gctaaaaaca actggggaca gaccgaacag cagcaggaac
    32101 cgtccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    32161 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    32221 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    32281 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    32341 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    32401 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    32461 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    32521 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    32581 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg tctgccaatc cgcgttgaac
    32641 tgcaggcgct gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    32701 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    32761 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    32821 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    32881 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    32941 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    33001 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    33061 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    33121 ctgcaattat cgtcgggaca gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    33181 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    33241 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    33301 aaaaaggggt cattacccag caagagatga aacgggcgct cattattacc gtcgtgctca
    33361 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    33421 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    33481 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    33541 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    33601 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    33661 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    33721 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    33781 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    33841 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    33901 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    33961 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    34021 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    34081 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    34141 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    34201 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    34261 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    34321 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    34381 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    34441 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    34501 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    34561 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgga
    34621 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    34681 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    34741 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    34801 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    34861 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaatcctc
    34921 gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta
    34981 tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt
    35041 catccttctc ctggtgacat aatccacatc aatcgaaaat gttaataaat ttgttgcgcg
    35101 aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaatggcgc gataacgctc
    35161 attttatgac gaggcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga
    35221 taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa
    35281 cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg
    35341 ctgaattcct cggtaccggg ttgttgattt tcttcggtgt gggttgcgtt gcagcactaa
    35401 aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg
    35461 caatggccat ctacctgacc gcaggggttt ccggcgcgca tcttaatccc gctgttacca
    35521 ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac
    35581 aagttgccgg cgctttctgt gctgcggctt tagtttacgg gctttactac aatttatttt
    35641 tcgacttcga gcagactcat cacattgttc gcggcagcgt tgaaagtgtt gatctggctg
    35701 gcactttctc tacttaccct aatcctcata tcaattttgt gcaggctttc gcagttgaga
    35761 tggtgattac cgctattctg atggggctga tcctggcgtt aacggacgat ggcaacggtg
    35821 taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat
    35881 ctatgggccc attgacaggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct
    35941 ttgcctggct ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact
    36001 tcctggtgcc gcttttcggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca
    36061 aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag gaaaccacaa
    36121 ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa
    36181 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    36241 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    36301 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    36361 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    36421 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    36481 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    36541 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    36601 tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga attgctgttt
    36661 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    36721 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    36781 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    36841 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    36901 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    36961 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    37021 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    37081 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga aatgaagttg
    37141 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    37201 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    37261 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    37321 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    37381 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    37441 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    37501 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    37561 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    37621 tacgcaggct ggaaaaaagc ggttaaacgc gcgatggcgt gggaagaaca cgacgaataa
    37681 tgtaaatgcc gaatgaagcg tttatgccgc atccggtagt cccgaaacgt gcgggggcaa
    37741 ccccgcacac atcaataatc cctcccttcc cctgtgctac acttcgcgcc attccttact
    37801 gcttagagtt tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc accgaatcag
    37861 cggcgctggc tggctacaaa tggttaggac gcggcgataa aaacaccgcg gacggcgcgg
    37921 cggtaaacgc catgcgtatt atgctcaacc aggtcaacat tgacggcacc atcgtcattg
    37981 gtgaaggtga aatcgacgaa gcaccgatgc tctacattgg tgaaaaagtc ggtactggtc
    38041 gcggcgacgc ggtagatatt gctgttgatc cgattgaagg cacgcgcatg acggcgatgg
    38101 gccaggctaa cgcgctggcg gtgctggcag taggcgataa aggctgcttc ctcaatgcgc
    38161 cggatatgta tatggagaag ctgattgtcg ggccgggagc caaaggcacc attgatctga
    38221 acctgccgct ggcggataac ctgcgcaatg tagcggcggc gctcggcaaa ccgttgagcg
    38281 aactgacggt aacgattctg gctaaaccac gccacgatgc cgttatcgct gaaatgcagc
    38341 aactcggcgt acgcgtattt gctattccgg acggcgacgt tgcggcctca attctcacct
    38401 gtatgccaga cagcgaagtt gacgtgctgt acggtattgg tggcgcgccg gaaggcgtag
    38461 tttctgcggc ggtgatccgc gcattagatg gcgacatgaa cggtcgtctg ctggcgcgtc
    38521 atgacgtcaa aggcgacaac gaagagaatc gtcgcattgg cgagcaggag ctggcacgct
    38581 gcaaagcgat gggcatcgaa gccggtaaag tattgcgcct gggcgatatg gcgcgcagcg
    38641 ataacgtcat cttctctgcc accggtatta ccaaaggcga tctgctggaa ggcattagcc
    38701 gcaaaggcaa tatcgcgact accgaaacgc tgctgatccg cggcaagtca cgcaccattc
    38761 gccgcattca gtccatccac tatctggatc gcaaagaccc ggaaatgcag gtgcacatcc
    38821 tctgattgat ttgatcgatt gagccttcca gtccttcggg actggaattt ttttgttcgg
    38881 agaacgaaga taaggcaagt caatcaaaac aggagaaaaa catggctgat tgggtaacag
    38941 gcaaagtcac taaagtgcag aactggaccg acgccctgtt tagtctcacc gttcacgccc
    39001 ccgtgcttcc gtttaccgcc gggcaattta ccaagcttgg ccttgaaatc gacggcgaac
    39061 gcgtccagcg cgcctactcc tatgtaaact cgcccgataa tcccgatctg gagttttacc
    39121 tggtcaccgt ccccgatggc aaattaagcc cacgactggc ggcactgaaa ccaggcgatg
    39181 aagtgcaggt ggttagcgaa gcggcaggat tctttgtgct cgatgaagtg ccgcactgcg
    39241 aaacgctatg gatgctggca accggtacag cgattggccc ttatttatcg attctgcaac
    39301 taggtaaaga tttagatcgc ttcaaaaatc tggtcctggt gcacgccgca cgttatgccg
    39361 ccgacttaag ctatttgcca ctgatgcagg aactggaaaa acgctacgaa ggaaaactgc
    39421 gcattcagac ggtggtcagt cgggaaacgg cagcggggtc gctcaccgga cggataccgg
    39481 cattaattga aagtggggaa ctggaaagca cgattggcct gccgatgaat aaagaaacca
    39541 gccatgtgat gctgtgcggc aatccacaga tggtgcgcga tacacaacag ttgctgaaag
    39601 agacccggca gatgacgaaa catttacgtc gccgaccggg ccatatgaca gcggagcatt
    39661 actggtaagc ggttacttat cgataaacgg cacgatgagc aaatccgcac tcatcttatt
    39721 gatcatcccg cgatatgccg gcatcaaacg gttgataaat gagtgatgat gaccacagac
    39781 aaggaggtcg cactgctctt tttgcataat ttccagcagt gtttccggca tttctccgcg
    39841 ttcaatacgc agttttgtct tcggccattg aatatttttc gtcagtttat acagcttgtt
    39901 atccgactta ttcttcaaca attgaagaat atcttctgtt gcagggaagt agatacccgg
    39961 atacaactcg cttaagccat catcaatatg aattaacgtc aggtgagcgt cattatgtct
    40021 ggcgagctcc agggctttat tcaccagtaa ggcatcttct tcattcccgg aaattgccac
    40081 gccaatgtgt ttataagcca tgtttaactc cttctaaagc aactccatca agctaacgaa
    40141 cgcagtgata actcaaaaat aatcatctcg gcctggcatg agaaagtgaa ggccgcatca
    40201 agctcaactt gcccctccag atccttaatc ttataggcca cgctggcccc ttcatctgca
    40261 gaggatgtag cggcggcaag ctctttcagt ttggtcataa aagactctgc ttcttcgcgg
    40321 gtggcaaaaa agcgcgaaaa cttactggta cagttgtcgt tatcaatcac cgtaccgata
    40381 tcaatcgcac atcctttagt actgcattta tctacgacat ctttcatagg gcacctctgt
    40441 atattcgcct ctctgtcgat tacgacttaa gaataatgcg ccgttaaggg aagaacaaac
    40501 aagtaatgat cttatggatt accatttttt ttgatattgc ttaaggtgaa gagttcagaa
    40561 ggattttccg ggtattgtca tttgcatcgt ggaaataaca atcccggaaa aaaatagcag
    40621 taagagatat attaaaggtt aattacttga tttattgtcg gctttatact tcacatcctg
    40681 agtatcttta ccatatttat tttcgccttg tgtgccaaca aacgcaccaa gatcgataag
    40741 catcatcacc agaatcaacg tcgggacaaa acgccccacc gcccattgcc agacacccgg
    40801 taaaatcgcc cagttacccg ccagcagcat ccacgccaca atcatcagaa atgcccatgc
    40861 gccggaacgc ccgcgatcat gcaagcgctt aacagttact gccgctgttg gccagagcaa
    40921 gcacacaagg caaaacgccg cggtctgaat atcgagtaga ttcttacccg ccagtgagaa
    40981 aagcaccagc atgcctgcga accacaggcc tatccaaatc cagaaatcac ggcgtccaat
    41041 acgcccttta aatgagaata accattgctg tatggtcatg taagttcctt gatggttgtc
    41101 ttttccagga ttctaccctt ttgacaaggg cgacagatat cgttttaatc ggagccagtc
    41161 ataacaaaag gtactgtcaa tgaagccagg gtgtacgctg ttttttctct tatgttctgc
    41221 attaaccgtt acaacagcgg cgcatgcaca aacaccagat acggcaacga ccgcgcctta
    41281 tctgctggct ggagccccta ctttcgatct ctccatcagc cagtttcgag aagactttaa
    41341 cagccagaat cccagcctgc cactgaacga atttcgtgcc atcgacagca gtcccgacaa
    41401 agccaatctc actcgtgctg caagtaaaat taatgagaac ttgtatgctt ctacagcgct
    41461 ggagcgcggt accttaaaaa tcaaaagcat tcaaatgacc tggctaccca tccaggggcc
    41521 agagcaaaaa gccgcgaaag cgaaagctca ggaatacatg gcagcggtga tccgcacact
    41581 caccccatta atgaccaaaa cacaaagcca gaaaaaactg cagtcgctac taacggcggg
    41641 gaaaaacaaa cgttattaca ccgagacaga aggtgcactg cgttatgttg tcgcggacaa
    41701 cggcgaaaag gggctgacct tcgctgttga accgattaag ctggcgctat ctgaatcgct
    41761 tgaaggtttg aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct ctatactgtt
    41821 tcacagacct gctgccctgc ggggcggcca tcttccttta ttcgcttata agcgtggaga
    41881 attaaaatgc gacatccttt agtgatgggt aactggaaac tgaacggcag ccgccacatg
    41941 gttcacgagc tggtttctaa cctgcgtaaa gagctggcag gtgttgctgg ctgtgcggtt
    42001 gcaatcgcac caccggaaat gtatatcgat atggcgaagc gcgaagctga aggcagccac
    42061 atcatgctgg gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac cggtgaaacc
    42121 tctgctgcta tgctgaaaga catcggcgca cagtacatca tcatcggtca ctctgaacgt
    42181 cgtacttacc acaaagaatc tgacgaactg atcgcgaaaa aattcgcggt gctgaaagag
    42241 cagggcctga ctccggttct gtgcatcggt gaaaccgaag ctgaaaatga agcgggcaaa
    42301 actgaagaag tttgcgcacg tcagatcgac gcggtactga aaactcaggg tgctgcggca
    42361 ttcgaaggtg cggttatcgc ttacgaacct gtatgggcaa tcggtactgg caaatctgca
    42421 actccggctc aggcacaggc tgttcacaaa ttcatccgtg accacatcgc taaagttgac
    42481 gctaacatcg ctgaacaagt gatcattcag tacggcggct ctgtaaacgc gtctaacgct
    42541 gcagaactgt ttgctcagcc ggatatcgac ggcgcgctgg ttggtggtgc ttctctgaaa
    42601 gctgacgcct tcgcagtaat cgttaaagct gcagaagcgg ctaaacaggc ttaagtctga
    42661 caggtgccgg atttcatatc cggcacttac tttccttaac tcttcgcttt aacgcaaaat
    42721 ctcacactga tgatcctgaa tttcctcggc tgaagcacgg ttaagcgtca gtagatttcg
    42781 ttgtgtcgcc agcaatacaa atgagttatc actctgccgt accatcgcca gcccgtagcg
    42841 tcccatatgt tcccgcgcct caggtacttc ttctgccagc atcataaatg ggctgcgttg
    42901 taccagttcg ctttccgtta cccgacgcgc caggtattca tgcccgcgca aaccacctgg
    42961 cagtggcaac cagcggctgc tgatgttcgc cagattgtta tccagctgtt tgcgcacatc
    43021 aggacgaata caagagatat gaatatgaaa atggttttgc gtacgcccgg tgcgggagtt
    43081 gatcgccaaa gaaaccgcgc gatcgggaac cggctggccg tattttttgc tcataaaatc
    43141 acgcgcctgc caagccaacc aaaagaagtt cggcgttgaa ggatcggtca acaaaggact
    43201 ttcagtaccg ttaatacgat acgttggcat caacagatat tgcagtgggc cattaagatc
    43261 ttttaaaacc acgtatccgg cattgggttt gacttccgca catggcgaag gattttgatt
    43321 ttgctgctga ttgggcaaac attcctcaag gacaatctta cgtaatgtat ccgactcttc
    43381 accggttaat ttccagtaac caataccggc agccacaacg gcgataacta tcatcaccaa
    43441 aaaaagaaga cccgcttttt tcatcttttt ttccctgtac ctcaaagaga tgcaagggta
    43501 acgcaaaatc gtgacaaata aaaaacccgg tcgcgagaaa ccaccgggtt tgataattat
    43561 cctggggaaa tcagcgtttg ctgatctgat cgaacgtacc gccgttagca aaatgctctt
    43621 tttgcgcttt cgtccagccg ccgaactctt catcaatggt gaataacttc agctttggaa
    43681 acgcattttc gtactttttc gccacctcag cgtcgcgcgg acggtagtag tttttcgcgg
    43741 caatttcctg accttctggc gagtagagat atttcaggta ggcttccgcc acctctttag
    43801 tacctttttt ctcgaccact ttatcgacca ccgacacggt tggctctgcg aggatagact
    43861 cactcggcgt gacgatttcg aatttatctt tccccagttc attcgctgcc agcagagctt
    43921 cgttttccca ggcaatcagt acatcgccaa ttccgcgctc gacaaaagtg ttagtggagc
    43981 cacgcgcgcc agaatccaga acttcgacgt ttttatacag tgcccgaaca aaatcctgtg
    44041 cttttgcctg atcgttgttg ttgtgatgca gcgcgtagcc ccaggctgcc aggtagttcc
    44101 agcgcgcgcc accagagctt ttcggattag gcgtgatcac cgaaacaccc ggtttaatca
    44161 gatcgttcca gtcatggatc tgcttcggat ttcccttacg taccaggaaa acaatggtgg
    44221 aagtgtacgg tgcggagtta tccggcagac gtttgatcca ctctttatca atccgcccgc
    44281 gttccgcaat tgcgtccacg tcataggcca gagccagcgt gacaacatca gcttcaatac
    44341 cgttgattac cgacgtcgct tgtttacctg agccaccgtg tgactgacga atcaccacgt
    44401 tatcaccagt ttgctgtttc cagtgggcgc tgaatgcctt gttgtactgt tcgtacaatt
    44461 cgcgcgttgg atcatatgaa acgttaagaa gctgaatatc ctttgccata acgctggttg
    44521 ccgccagcaa aaatgttaac cctacgcccc acttgttcat cgcccgactc tcttatgttg
    44581 tgttgtgatg agcaaagcgt gccagaaggt taaccaaaca ttaaagaata aaaaaagatt
    44641 ggctataact tgcgggcata tgttgaggga ttaaaaaggc ggaagaaatc cgcctcatat
    44701 tgctgacaaa gtgcgctttg tccatgccgg atgcggcgcg aacgccttat ccggcctaca
    44761 aaagtttgca aattcaataa attgcagaat tcatgtaggc ctgataagcg aagcgcatca
    44821 ggcatttttg cttctgtcat cggtttcagg gtaaaggaat ctgccttttt ccgaaatcat
    44881 taatacagtt ttttcgcgca gtccagccag tcacctttga acggacgctt catgttttcg
    44941 atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac acaacgaccg
    45001 ccgtaacctg ccagcagcag atcgatagcg taagcgccca tacgggaagc cagaatacgg
    45061 tcgtaaggca ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca
    45121 cgaccggttt ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta
    45181 atcgccacga tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg
    45241 tcttcacggc tgaattcaac ttccggaacc acaacgaatt cacagccacc ggcaatggcc
    45301 gcagccaacg tcagatctcc acaataacgg cccatcactt ccaccacgga aatacgctgg
    45361 tgagaagaag aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg
    45421 aagaaaccga tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcagaccg
    45481 atgcacggga agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg
    45541 ataaccacca gcgcgtcgat accacgtttt ttcaggtttt cgatagccac ggcgcggatg
    45601 ttctcgtcgc ggaattccgg gaaacgcgca gaaccgagga acgtaccgcc acggttgatc
    45661 atgtcagaca cgctgtaacg gtctagctgt accatacggt cttcatacag acccagatag
    45721 ccgtcataaa tacccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga
    45781 attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat tttcttaatc
    45841 atgactacct ctgaactttg gaatgcaaaa tgaaatctgt tgccggaagt cttcttgcac
    45901 atcgaagtga tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc
    45961 aggtcaggcc aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact
    46021 gattatgaaa gcatagaccg tttaccctcc ctgggtacga cggaacaggg gtcctgatgg
    46081 ataattacat ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc
    46141 atatgtgcct gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg
    46201 gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc
    46261 acgatatcaa taatttcttg ccgttcctca tcaggcaatg cgcgatccag taatgactgt
    46321 accgcctcat atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca
    46381 aacagagcat cggcgcgatg ccagccgtac caggacaacc ccagcgccag cagaattgcg
    46441 ccgttcatca taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc
    46501 gtccgccgca ccacccaacg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca
    46561 attgtcacga taaccccgac gcctggatct gtcatcggtg ttggagatat cagatgttga
    46621 atacccgtca aaaacaggaa tagtgccgaa ccggagataa acatactttg cgccagcgcc
    46681 gcgagggact cagctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa
    46741 tatcgcacca ccaataaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc
    46801 gcggcgagaa tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc
    46861 agcgaagcca tcgccgtcgc agcaatcgcc gcccgactga ccagccgtcc ataagattga
    46921 ttcataaata ctcccgctat caactgacgc tagtataacg gaagcaaatc atctgcaatg
    46981 cattaagcag caggcaaatt gaggataaaa aaaaccccca cagcatgtgg gggaagacag
    47041 ggatggtgtc tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact
    47101 caatagcttc aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact gctccattcg
    47161 ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc gttaacaggc gatacatttg
    47221 gttgcggact tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc
    47281 ctgtgcgcgc acagcgtttt catcaaaatt ttctgcggtg acaaggcgat gcattgtctc
    47341 cagttcgcta acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat
    47401 ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg
    47461 ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct
    47521 taatgaactg actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg
    47581 ctcccaaaat ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca
    47641 tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt
    47701 tctgcctcgg aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc
    47761 tgacttccct attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg
    47821 atggggaaca ggcgcttgat cttctggacg acagcattga tttacttttg cttgacgtaa
    47881 tgatgccgaa gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc
    47941 ctgtcattat gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg
    48001 gcgcagatga ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg
    48061 cgatcctgcg ccgttcgcac tggagcgagc aacagcaaaa caacgacaac ggttcaccga
    48121 cactggaagt tgatgcctta gtgctgaatc caggccgtca ggaagccagc ttcgacgggc
    48181 aaacgctgga gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg
    48241 gtcaggtggt ttcccgtgaa catttaagcc aggaagtgtt gggcaaacgc ctgacgcctt
    48301 tcgaccgcgc tattgatatg cacatttcca acctgcgtcg taaactgccg gatcgtaaag
    48361 atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta tctgatggtt tctgcttcat
    48421 gataggcagc ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt
    48481 gatgttggtt ttgatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag
    48541 cgaacagcgt caggggctga tgattgagca gcatgtcgaa gcggagctgg cgaacgatcc
    48601 gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc
    48661 aggacagcgt ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga
    48721 aatgcagatc attcgtaact ttattggtca ggccgataac gccgatcatc cgcagaagaa
    48781 aaagtatggc cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg aagataatta
    48841 ccaactttat ctgattcgtc cggccagcag ttctcaatcc gatttcatta acttactgtt
    48901 tgaccgcccg ctattactgc tgattgtcac catgttggtc agtacgccgc tgctgttgtg
    48961 gttggcctgg agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc
    49021 ccagggaaac ttacgccagc acccggaact ggaagcgggg ccacaggaat tccttgccgc
    49081 aggtgccagt tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcagcg
    49141 tctgctttct gatatctctc acgagctgcg caccccgctg acgcgtctgc aactgggtac
    49201 ggcgttactg cgccgtcgta gcggtgaaag caaggaactg gagcgtattg aaaccgaagc
    49261 gcaacgtctg gacagcatga tcaacgatct gttggtgatg tcacgtaatc agcaaaaaaa
    49321 cgcgctggtt agcgaaacca tcaaagccaa ccagttgtgg agtgaagtgc tggataacgc
    49381 ggcgttcgaa gccgagcaaa tgggcaagtc gttgacagtt aacttcccgc ctgggccgtg
    49441 gccgctgtac ggcaatccga acgccctgga aagtgcgctg gaaaacattg ttcgtaatgc
    49501 tctgcgttat tcccatacga agattgaagt gggctttgcg gtagataaag acggtatcac
    49561 cattacggtg gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac agattttccg
    49621 tccgttctat cgtaccgatg aagcacgcga tcgtgaatct ggcggtacag gtttggggct
    49681 ggcgattgtt gaaaccgcca ttcagcagca tcgtggctgg gtgaaggcag aagacagccc
    49741 gctgggcggt ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaacttcgca
    49801 tttgcaggca ggataaggcg tttacgccgc atccggcatt tgagcaggat gcctgatgcg
    49861 acgctgatag cgtcttatca ggcctacact ccgggggtta ttttccccac aaccggcggg
    49921 aaaaatcttc gatcttgccg ctcagtcggc gcttttgcat cgtccgcgtc cagcttttcg
    49981 ataacccggc agcggagagt aaacggtgat attgatcgtc atcaaacggc atatgccatg
    50041 cgatggcggc agcctcctgc acggtcacat cgctgacacg ggaaaccaac tccagcggcg
    50101 cgtccgcaga cactttccct ggcgctatca cgctatacaa ccaccccacc ttacctgtgt
    50161 tttgcatcaa ctgcgctata tcgctgatat caaaatggta attgagttta tagcaaggcg
    50221 agcgcggctg gctgacctga attaatgcct ctccccagcg gaaaatatcg cccatataaa
    50281 cattactttc cgtcaggccg tcggttgaga ggttttcacc aaacgcaggc gcaacaaaca
    50341 actccgcctg ttccggaaat tcccgcgccc agtagagata atgctcacga ggataatgac
    50401 acagcgctct gtctggcccg ccgtgaacct ttttctccgc ctgctcgtca ccttccagcc
    50461 ccagctccgt cagcatcaac tcaccatcaa cctggatttt agcaattgcg ctgggtttgc
    50521 cttcgggata agcctgaatc ttgcctgtgt atacatcaac cggatatcgc atctgttccc
    50581 tctccacgag aaatttcccc attagaccat atttatttta tgtcaggcgt gctgttttcg
    50641 cggtaagtat ccaggcagga actcaatagc aaaccggacg aacgttagtt catccggctg
    50701 tggggattaa ggaagcaggt ttatttcacc gtacctaaaa tttcgatttt cgctgctctg
    50761 gctttgactt tacgtgacca gatagaggta agaattggca ccagaatcga agtcacaatg
    50821 accgccgtcg ctaccagcga agttgctgcc ggagccatcg gtttaaacgc aggcaccatt
    50881 tctgcaatca gcacaggtgt cgctaccgcg gcccctgcgg aactggaagc ggcaattccg
    50941 gcagtgccat cgccaccgcc aatcaattta tcggcgataa tcaacggaat accggtcacg
    51001 ataattactg ccacacccaa caggatcccc agcaaaccag tctgggcaat cacagtcaaa
    51061 tcaatggtat tgcccagcgc gaaggcaaag aatggaatca gcgtttgcac cgctttgctg
    51121 aaaaattctc gcaattcagg gtcaaggttc cccagggcaa agcccaccag gaacggtaat
    51181 actgcgccga cgaaaacatg cggttcaaac gaggcaatcc cggcagtgcc cagaataatc
    51241 atcgtcatga gcggcccgga ctccaacgac atcaacacaa atgccccagc ttcttctttt
    51301 gtgccgtact gctgcatgat ggaagcgtaa agtccgccat tggtcatatc catcgccgcc
    51361 accagcgcca gcgttgaaag tccggcaaag aatccaactt caacaccatg ttccggaata
    51421 atacgcgagg caattgccgc aaccacccac gcgacggcaa ttttagttac caccagcgta
    51481 ccggatttac gcagtaccgt tcccgtcgcg cttaatttta ttgacgcccc catgcaaaaa
    51541 aaccacaccg ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa tgatccaaaa
    51601 tatttccccg cccccggcga gaaggtgtgg cacagtgcgc caaggaatag cgggacgagc
    51661 atcatccccc ccgggatttt ctcaatcgag cgttttatct gcatttccat cacctgtcgg
    51721 catattttta agggctgatg cgtaattcct caatggagca tcagtgttga tggaaacgaa
    51781 gatagacatt catttaatca ataaaagtga tcaaaaccac attaattgaa acgctgtttt
    51841 atttttatcg gatcattgtt tttattttga aattgatcac aaaaaaacca ccgccgttgg
    51901 cgatggttca ttttttaagc tgatatgcgg cctatacgcc tcattgcagc aggcggcaaa
    51961 tgattatttt ttcgccgcaa aacgtgccgc tgcttcgtcc cagttcacca cgttccagaa
    52021 ctctttaatg tagtccggac ggcggttctg gaatttcagg tagtaagcat gttcccacac
    52081 atccaggccc ataatcggga agccggaagc gccagaaata gcttcaccca tcagcggaga
    52141 atcctggtta gcagtagaaa ccaccgccag tttatcgcct ttcagcacca gccatgccca
    52201 gccggaacca aagcgggaag ctgccgcttt ttcaaattct gctttgaagt tatcaacgga
    52261 gccgaagtca cgttcgatag ccgctttcag gtcaccctgc agggtggtgc cttttttcag
    52321 acctttccag aacaggctgt ggttagcgtg accgccagcg ttgttgcgca gtacggtttt
    52381 cttgtctgct ggcagctggt ccagtttggt gatcagctct tcaaccggca ggttggcaaa
    52441 ttctggcagg ctttccagcg ccgcgttggc gttgtttacg taggtctgat ggtgtttggt
    52501 gtggtggatt tccatggtct gcttatcgaa gtgcggttcc agggcatcgt aagcatacgg
    52561 cagggatggc agggtatagc tcatattcat ctccagtatt gtcgggcggc cgattgttaa
    52621 tgccgcgtaa gcagttggtt cattatagtt aattaaatga tattgaaaat gattatcaat
    52681 gccgtacttt tcgtaagggt atggttttgc aggaaaatgc ccgagatgtg aagcaaatca
    52741 cccacttaat gccgtgattg ccagtaaatc gacaacggcg gcaacaggcg aaaggttaat
    52801 cgacagcacg atttttacac tcatctcgtc ggagatgtga cgcgacgaaa aatgatgagg
    52861 ataagaagat gagtaacgcg attacgatgg ggatattttg gcatttgatc ggcgcggcca
    52921 gtgcagcctg tttttacgct ccgttcaaaa aagtaaaaaa atggtcatgg gaaaccatgt
    52981 ggtcagtcgg tgggattgtt tcgtggatta ttctgccgtg ggccatcagc gccctgttac
    53041 taccgaattt ctgggcgtat tacagctcgt ttagtctctc tacgctactg cctgtttttc
    53101 tgttcggcgc tatgtggggg atcggtaata tcaactacgg cctgaccatg cgttatctcg
    53161 gcatgtcgat gggaattggc atcgccattg gcattacgtt gattgtcggt acgctgatga
    53221 cgccaattat caacggcaat ttcgatgtgt tgattagcac cgaaggcgga cgcatgacgt
    53281 tgctcggcgt tctggtggcg ctgattggcg tagggattgt aactcgcgcc gggcagttga
    53341 aagagcgcaa gatgggcatt aaagccgaag agttcaatct gaaaaaaggg ctggtgctgg
    53401 cggtgatgtg cggcattttc tctgccggga tgtcctttgc gatgaacgcc gcaaaaccga
    53461 tgcatgaagc cgctgccgca cttggcgtcg atccactgta tgtcgctctg ccaagctatg
    53521 ttgtcatcat gggcggcggc gcgatcatta acctcggttt ctgttttatt cgtctggcaa
    53581 aagtgaagga tttgtcgcta aaagccgact tctcgctggc aaaatcgctg atcattcaca
    53641 atgtgttact ctcgacactg ggcgggttga tgtggtatct gcaattcttt ttctatgcct
    53701 ggggccacgc ccgcattccg gcgcagtatg actacatcag ttggatgctg catatgagtt
    53761 tctatgtatt gtgcggcggt atcgtcgggc tggtgctgaa agagtggaac aatgcaggac
    53821 gccgtccggt aacggtgttg agcctcggtt gtgtggtgat tattgtcgcc gctaacatcg
    53881 tcggcatcgg catggcgaat taatctttct gcgaattgag atgacgccac tggctgggcg
    53941 tcatcccggt ttcccgggta aacaccaccg aaaaatagtt actatcttca aagccacatt
    54001 cggtcgaaat atcactgatt aacaggcggc tatgctggag aagatattgc gcatgacaca
    54061 ctctgacctg tcgcagatat tgattgatgg tcattccagt ctgctggcga aattgctgac
    54121 gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt atccagcgca aagggacttt
    54181 tcaggctagc cgccagccgg gtaatcagct tatccagcaa cgtttcgctg gatgttggcg
    54241 gcaacgaatc actggtgtaa cgatggcgat tcagcaacat caccaactgc ccgaacagca
    54301 actcagccat ttcgttagca aacggcacat gctgactact ttcatgctca agctgaccga
    54361 taacctgccg cgcctgcgcc atccccatgc tacctaagcg ccagtgtggt tgccctgcgc
    54421 tggcgttaaa tcccggaatc gccccctgcc agtcaagatt cagcttcaga cgctccgggc
    54481 aataaataat attctgcaaa accagatcgt taacggaagc gtaggagtgt ttatcgtcag
    54541 catgaatgta aaagagatcg ccacgggtaa tgcgataagg gcgatcgttg agtacatgca
    54601 ggccattacc gcgccagaca atcaccagct cacaaaaatc atgtgtatgt tcagcaaaga
    54661 catcttgcgg ataacggtca gccacagcga ctgcctgctg gtcgctggca aaaaaatcat
    54721 ctttgagaag ttttaactga tgcgccaccg tggctacctc ggccagagaa cgaagttgat
    54781 tattcgcaat atggcgtaca aatacgttga gaagattcgc gttattgcag aaagccatcc
    54841 cgtccctggc gaatatcacg cggtgaccag ttaaactctc ggcgaaaaag cgtcgaaaag
    54901 tggttactgt cgctgaatcc acagcgatag gcgatgtcag taacgctggc ctcgctgtgg
    54961 cgtagcagat gtcgggcttt catcagtcgc aggcggttca ggtatcgctg aggcgtcagt
    55021 cccgtttgct gcttaagctg ccgatgtagc gtacgcagtg aaagagaaaa ttgatccgcc
    55081 acggcatccc aattcacctc atcggcaaaa tggtcctcca gccaggccag aagcaagttg
    55141 agacgtgatg cgctgttttc caggttctcc tgcaaactgc ttttacgcag caagagcagt
    55201 aattgcataa acaagatctc gcgactggcg gtcgagggta aatcattttc cccttcctgc
    55261 tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata cgctgtggtt aacgcgccag
    55321 tgagacggat actgcccatc cagctcttgt ggcagcaact gattcagccc ggcgagaaac
    55381 tgaaatcgat ccggcgagcg atacagcaca ttggtcagac acagattatc ggtatgttca
    55441 tacagatgcc gatcatgatc gcgtacgaaa cagaccgtgc caccggtgat ggtatagggc
    55501 tgcccattaa acacatgaat acccgtgcca tgttcgacaa tcacaatttc atgaaaatca
    55561 tgatgatgtt caggaaaatc cgcctgcggg agccggggtt ctatcgccac ggacgcgtta
    55621 ccagacggaa aaaaatccac actatgtaat acggtcatac tggcctcctg atgtcgtcaa
    55681 cacggcgaaa tagtaatcac gaggtcaggt tcttacctta aattttcgac ggaaaaccac
    55741 gtaaaaaacg tcgatttttc aagatacagc gtgaattttc aggaaatgcg gtgagcatca
    55801 catcaccaca attcagcaaa ttgtgaacat catcacgttc atctttccct ggttgccaat
    55861 ggcccatttt cctgtcagta acgagaaggt cgcgaattca ggcgcttttt agactggtcg
    55921 taatgaaatt cagcaggatc acattatgac ctttcgcaat tgtgtcgccg tcgatctcgg
    55981 cgcatccagt gggcgcgtga tgctggcgcg ttacgagcgt gaatgccgca gcctgacgct
    56041 gcgcgaaatc catcgtttta acaatgggct gcatagtcag aacggctatg tcacctggga
    56101 tgtggatagc cttgaaagtg ccattcgcct tggattaaac aaggtgtgcg aggaagggat
    56161 tcgtatcgat agcattggga ttgatacctg gggcgtggac tttgtgctgc tcgaccaaca
    56221 gggtcagcgt gtgggcctgc ccgttgctta tcgcgatagc cgcaccaatg gcctaatggc
    56281 gcaggcacaa caacaactcg gcaaacgcga tatttatcaa cgtagcggca tccagtttct
    56341 gcccttcaat acgctttatc agttgcgtgc gctgacggag caacaacctg aacttattcc
    56401 acacattgct cacgctctgc tgatgccgga ttacttcagt tatcgcctga ccggcaagat
    56461 gaactgggaa tataccaacg ccacgaccac gcaactggtc aatatcaata gcgacgactg
    56521 ggacgagtcg ctactggcgt ggagcggggc caacaaagcc tggtttggtc gcccgacgca
    56581 tccgggtaat gtcataggtc actggatttg cccgcagggt aatgagattc cagtggtcgc
    56641 cgttgccagc catgataccg ccagcgcggt tatcgcctcg ccgttaaacg gctcacgtgc
    56701 tgcttatctc tcttctggca cctggtcatt gatgggcttc gaaagccaga cgccatttac
    56761 caatgacacg gcactggcag ccaacatcac caatgaaggc ggggcggaag gtcgctatcg
    56821 ggtgctgaaa aatattatgg gcttatggct gcttcagcga gtgcttcagg agcagcaaat
    56881 caacgatctt ccggcgctta tctccgcgac acaggcactt ccggcttgcc gcttcattat
    56941 caatcccaat gacgatcgct ttattaatcc tgagacgatg tgcagcgaaa ttcaggctgc
    57001 gtgtcgggaa acggcgcaac cgatcccgga aagtgatgct gaactggcgc gctgcatttt
    57061 cgacagtctg gcgctgctgt atgccgatgt gttgcatgag ctggcgcagc tgcgcggtga
    57121 agatttctcg caactgcata ttgtcggcgg aggctgccag aacacgctgc tcaaccagct
    57181 atgcgccgat gcctgcggta ttcgggtgat cgccgggcct gttgaagcct cgacgctcgg
    57241 caatatcggc atccagttaa tgacgctgga tgaactcaac aatgtggatg atttccgtca
    57301 ggtcgtcagc accaccgcga atctgaccac ctttacccct aatcctgaca gtgaaattgc
    57361 ccactatgtg gcgcagattc actctacacg acagacaaag gagctttgcg catgaccact
    57421 caactggaac aggcctggga gctagcgaaa cagcgtttcg cggcggtggg gattgatgtc
    57481 gaggaggcgc tgcgccaact tgatcgttta cccgtttcaa tgcactgctg gcagggcgat
    57541 gatgtttccg gttttgaaaa cccggaaggt tcgctgaccg gggggattca ggccacaggc
    57601 aattatccgg gcaaagcgcg taatgccagt gagctacgtg ccgatctgga acaggctatg
    57661 cggctgattc cggggccgaa acggcttaat ttacatgcca tctatctgga atcagatacg
    57721 ccagtctcgc gcgaccagat caaaccagag cacttcaaaa actgggttga atgggcgaaa
    57781 gccaatcagc tcggtctgga ttttaacccc tcctgctttt cgcatccgct aagcgccgat
    57841 ggctttacgc tttcccatgc cgacgacagc attcgccagt tctggattga tcactgcaaa
    57901 gccagccgtc gcgtttcggc ctattttggc gagcaactcg gcacaccatc ggtgatgaac
    57961 atctggatcc cggatggtat gaaagatatc accgttgacc gtctcgcccc gcgtcagcgt
    58021 ctgctggcag cactggatga ggtgatcagc gagaagctaa accctgcgca ccatatcgac
    58081 gccgttgaga gcaaattgtt tggcattggc gcagagagct acacggttgg ctccaatgag
    58141 ttttacatgg ggtatgccac cagccgccag actgcgctgt gcctggacgc cgggcacttc
    58201 cacccgactg aagtgatttc cgacaagatt tccgccgcca tgctgtatgt gccgcagttg
    58261 ctgctgcacg tcagccgtcc ggttcgctgg gacagcgatc acgtagtgct gctggatgat
    58321 gaaacccagg caattgccag tgagattgtg cgtcacgatc tgtttgaccg ggtgcatatc
    58381 ggccttgact tcttcgatgc ctctatcaac cgcattgccg cgtgggtcat tggtacacgc
    58441 aatatgaaaa aagccctgct gcgtgcgttg ctggaaccta ccgctgagct gcgcaagctg
    58501 gaagcggcgg gcgattacac tgcgcgtctg gcactgctgg aagagcagaa atcgttgccg
    58561 tggcaggcgg tctgggaaat gtattgccaa cgtcacgata cgccagcagg tagcgaatgg
    58621 ctggagagcg tgcgggctta tgagaaagaa attttgagtc gccgcgggta aacactgccg
    58681 gatgcggcgc gagcgcctta tccggcctac gggtcgtgca tccgacaaca ccgaatttac
    58741 aggaacacag aacatgcaaa acattactca gtcctggttt gtccagggaa tgatcaaagc
    58801 caccaccgac gcctggctga aaggctggga tgagcgcaac ggcggcaacc tgacgctacg
    58861 cctggatgac gccgatatcg caccatatca cgacaatttc caccaacaac cgcgctatat
    58921 cccgctcagc cagcccatgc ctttactggc aaatacaccg tttattgtca ccggctcggg
    58981 caaattcttc cgtaacgtcc agcttgatcc tgcggctaac ttaggcatcg taaaagtcga
    59041 cagcgacggc gcgggctacc acattctttg ggggttaacc aacgaagccg tccccacttc
    59101 cgaacttccg gctcacttcc tttcccactg cgagcgcatt aaagccacca acggcaaaga
    59161 tcgggtgatc atgcactgcc acgccaccaa cctgatcgcc ctcacctatg tacttgaaaa
    59221 cgacaccgcg gtcttcactc gccaactgtg ggaaggcagc accgagtgtc tggtggtatt
    59281 cccggatggc gttggcattt tgccgtggat ggtgcccggc acggacgaaa tcggccaggc
    59341 gaccgcacaa gagatgcaaa aacattcgct ggtgttgtgg cccttccacg gcgtcttcgg
    59401 cagcggaccg acgctggatg aaaccttcgg tttaatcgac accgcagaaa aatcagcaca
    59461 agtattagtg aaggtttatt cgatgggcgg catgaaacag accatcagcc gtgaagagtt
    59521 gatagcgctc ggcaagcgtt tcggcgttac gccactcgcc agtgcgctgg cgctgtaagg
    59581 agcaaacatg atccgcaaag cctttgtcat gcaggtaaac cccgacgccc acgaagagta
    59641 tcagcgtcgg cataatccca tctggccaga actggaagca gtgctgaaat ctcacggtgc
    59701 gcataactac gccatctatc tcgacaaagc gcgtaatctg ctgtttgcca tggtagagat
    59761 tgaatctgaa gaacgctgga atgcggttgc cagcactgat gtttgccaac gttggtggaa
    59821 atatatgacc gatgttatgc ccgctaaccc ggataacagc ccggtgagta gcgagctgca
    59881 agaagtgttt tacctgccgt aattgcccaa tgtgccagag atgcctccgg cacaatattt
    59941 taaatttcga tttgaaaaat taaacatatt acatctcccg gtaagttata tttccctgat
    60001 acattgtgag taaatcacaa aaataatgaa taacccatta atgattcatg tggtttattt
    60061 aaataaccat tatgtgcatt actccgcaaa tctgaccttt cactctgtct caaattgtca
    60121 ttgtttaccg acatatcgcc tctcatcact taatcgctcc accattacaa ttgattaaaa
    60181 tatattttat ggagtccgat taatggcagc tcttactgca agctgtattg acctgaatat
    60241 tcagggcaat ggcgcttatt ccgttctgaa gcagttggcg acaatagcgt tacaaaacgg
    60301 ttttatcacc gactcacacc agttcctgca aaccctgctg ctgcgcgaaa aaatgcactc
    60361 tactggattt ggttccggtg tcgccgtgcc gcacggtaaa agcgcctgcg ttaaacaacc
    60421 gttcgtatta ttcgcccgca aagcgcaggc tattgactgg aaagccagcg atggcgaaga
    60481 cgtcaattgc tggatctgcc tcggcgtgcc gcaaagcggc gaagaggatc aggtcaaaat
    60541 catcggcaca ctgtgtcgca aaattattca caaggaattt attcatcaac tgcaacaggg
    60601 cgataccgac caggtgcttg ccttgttaaa tcaaaccctc agctcataag gaagtggcga
    60661 tggagtcatc cttacgtatt gtcgcgatca ccaactgccc cgccgggatc gctcacacct
    60721 acatggtggc ggaagccctg gaacagaaag cccgttctct cggtcatacc ataaaagtgg
    60781 aaactcaagg gtccagtggc gttgaaaacc gcttatccag cgaagagatt gccgctgccg
    60841 attacgtcat tctcgctacc gggcgtggcc tgagcggtga tgatcgcgcg cggtttgccg
    60901 ggaagaaagt ttatgagatt gccatctccc aggcgttgaa aaatatcgac cagattttca
    60961 gcgaattacc gacaaactcg cagctttttg ccgcagatag cggcgtgaag cttggcaaac
    61021 aggaagtgca aagcggtagc gtgatgagcc acctgatggc gggcgtctct gcggcgctgc
    61081 cgtttgtcat cggcggtggt atcctggtgg cgcttgccaa catgctggtg cagttcggtt
    61141 tgccgtatac cgatatgtcg aaaggcgcgc cgtcgtttac ctgggtagtt gaatccatcg
    61201 gttaccttgg cttcaccttt atgatcccca tcatgggcgc ttatattgcc tcgtcgattg
    61261 ccgataaacc ggctttcgct ccggcgtttc tggtttgcta tctggcgaac gacaaagcgc
    61321 tgctcggcac ccagtcgggt gcgggattcc tcggcgcggt ggtgctgggg ctggcgattg
    61381 gctatttcgt cttctggttc cgcaaagtgc gccttggcaa agcattacaa ccgctgctcg
    61441 gttcaatgct gatcccgttc gttactctgc tggttttcgg cgtgctcact tactacgtta
    61501 tcgggccggt gatgtccgac ctcatgggcg ggctgctcca cttcctgaat accattcctc
    61561 catcaatgaa gtttgcggca gcgtttctgg tcggtgcgat gctggcgttc gatatgggcg
    61621 gccccatcaa taaaaccgcc tggttcttct gcttctcact gctggaaaaa cacatttatg
    61681 actggtacgc catcgtcggc gttgtcgcgc tgatgccgcc cgtcgcggcg ggtcttgcga
    61741 cgttcattgc gcccaaactg tttacccgac aggaaaaaga ggctgccagt agtgccattg
    61801 tggtcggtgc cacagtcgca accgaaccgg ctattcctta cgctctggca gctccgttgc
    61861 cgatgattac cgccaatacg ctggcgggcg gcattaccgg cgtgctggtg atcgctttcg
    61921 gaatcaaacg tctggcacct ggtttgggta tctttgaccc gctgatcggc ctgatgtcgc
    61981 cggtgggttc gttttatctg gtgctggcga ttggcctcgc gctgaacatt tcattcatta
    62041 tcgtcctgaa aggattgtgg ttacgccgca aagcgaaagc cgcgcaacag gagcttgtcc
    62101 atgaacattg agttactgca acagttgtgc gaagccagcg ccgtcagcgg cgatgaacag
    62161 gaagttcgcg acattctgat aaacacgctg gaaccttgcg tgaatgaaat caccttcgat
    62221 ggtctgggca gctttgttgc ccgtaagggg aataaaggtc caaaagttgc cgttgtcgga
    62281 catatggatg aagtcggctt tatggtcacc cacatcgacg agagcggttt tctgcgtttt
    62341 accaccattg gcggctggtg gaatcagtcg atgctcaacc accgggtaac catacgcaca
    62401 cacaagggag tgaaaatccc tggtgtgatt ggttccgtcg cgcctcatgc gttaacggaa
    62461 aagcaaaagc aacaaccgct gtcatttgat gagatgttca ttgatattgg cgcgaacagt
    62521 cgcgaagagg tggaaaagcg cggcgtggaa attggtaatt ttattagccc ggaagccaat
    62581 tttgcctgct ggggcgaaga taaagtggtc ggcaaggcgt tggataaccg catcggctgc
    62641 gcaatgatgg ctgaactatt gcagacggtg aataatcccg aaattacgct gtatggcgtt
    62701 ggcagtgtgg aagaagaagt tgggctacgc ggggcgcaaa cctcggcgga acacattaaa
    62761 ccggacgtcg tgatcgtgtt ggataccgcc gtagcgggcg atgttccggg cattgataac
    62821 attaaatacc cgctgaaact gggccagggg ccggggctga tgctgtttga caagcgctac
    62881 ttccccaacc agaaactggt agcagcgtta aaaagctgtg ccgcacataa cgatttaccg
    62941 ctgcaatttt ccaccatgaa aaccggtgcg acggatggcg ggcgctacaa cgtgatgggc
    63001 ggcgggcgtc cggttgtcgc gctgtgtctg ccaactcgtt atctgcacgc caacagcggt
    63061 atgatttcaa aagccgatta cgaagctctg ctcacgctga tacggggttt tctgacgacc
    63121 ttaactgcgg agaaagtcaa cgcgtttagc cagttccgtc aggtggatta atgctaaacg
    63181 aacgccagtt aaagattgtc gatctgctgg agcaacagcc gcgcacgcct ggcgagctgg
    63241 cgcaacagac tggcgtttca ggcaggacca tcctgcgtga tattgactat ctcaacttca
    63301 cccttaacgg caaagcccgc attttcgcca gtggcagtgc gggctatcag ctggaaatct
    63361 tcgagcgccg cagctttttt cagttgctgc aaaagcacga taacgacgat cggctgctgg
    63421 cgctgttatt actgaatact ttcactcccc gtgcgcaact cgcctcggcg cttaatttgc
    63481 cagaaacgtg ggtagcagag cgtctgcccc ggttaaaaca gcgttatgaa cgcacttgtt
    63541 gcctggccag ccgccctggt ttgggccatt tcattgatga gacagaagag aaacgcgtta
    63601 tcttgctggc gaacttgctg cgcaaagatc cgtttttaat tccgctggcg ggcataacac
    63661 gagacaacct tcagcattta tccacggcct gcgacaacca acaccgctgg ccgctcatgc
    63721 agggtgatta tctctccagc ctgattctgg cgatttacgc cctgcgtaat caactgaccg
    63781 atgagtggcc gcaatatccc ggtgacgaga taaaacaaat cgttgaacat agcggtctgt
    63841 ttcttggtga taacgctgta agaaccctga cgggtttgat agagaaacag catcagcaag
    63901 cgcaggtaat ttcagccgat aatgtgcagg ggttgctgca aagggtgccg ggcatcgcgt
    63961 cattgaatat tattgatgcg cagctggttg agaatattac cgggcattta ttacgttgcc
    64021 ttgccgcacc agtgtggatt gctgagcacc gccagagcag catgaataac ctgaaagccg
    64081 cctggcctgc ggcctttgat atgagtctgc actttattac gctactgcgt gaacagctcg
    64141 atattcccct tttcgacagc gatctgatcg gtttgtattt tgcctgtgcg ctggagcggc
    64201 atcaaaacga acgccagccg atcattttgc tctcggacca gaacgcgatt gccactatta
    64261 atcagctcgc cattgagcgc gatgttttaa attgtcgggt aattattgcc cgtagcttaa
    64321 gcgaacttgt tgccattcgc gaagagattg agccgttatt gatcattaac aacagccatt
    64381 atttactgga tgacgcggta aataattaca tcaccgtaaa aaatatcatt acggctgccg
    64441 gtatcgaaca aataaaacat tttctggcga cggcatttat tcgccaacag ccggagcgtt
    64501 ttttctctgc ccccggaagt tttcattatt cgaatgtacg cggtgaaagc tggcaacata
    64561 ttacccggca aatttgtgcg caattagtcg cacaacacca tattaccgcc gatgaagcac
    64621 aacgcatcat cgcccgcgaa ggcgaaggtg aaaacctgat tgttaatcgc ctcgccatcc
    64681 cacattgctg gagcgaacag gagcgacgtt ttcgtggatt ttttattacc ctcgcccaac
    64741 cagttgaggt gaataacgaa gtcattaacc atgtcttgat cgcctgcgcc gccgccgatg
    64801 cgcgtcatga gctaaaaata tttagctatc tggcaagcat attgtgtcag catccggcgg
    64861 agattattgc cgggttaaca ggatatgagg catttatgga gttacttcac aaggggtgaa
    64921 ctgtgtcgga tgcgccgcat ccgacactgc atttatcatc aaaaacgttt aacgcaggct
    64981 attatagtca tcaaccattt cgttgtactc gcgtaatgct ctcgggaaag agtcttcgac
    65041 catccagctt gagtttgcgt cctgaagttg ctctttatcc cagtcagagt acgggacttt
    65101 atctctgatg cggcgaacgt attttttagc ctcgagttga tactggcctg ccgaattaat
    65161 aaacgagaaa ttcatgccgc ctttatccgc ttctttggcc tgcgccacta gcgtttccag
    65221 ttcagacact ttcttcattg ccgcttctgc atcaaactta tcttgcgata tcaggttatt
    65281 aatttgtttc gctgaaatca tgactgccag agagtagtag tggaatgttt tcccttctct
    65341 ttcttcaata tttttcagtt ccgcaagctg acgcttatca ttaatttcct gaatcgccgc
    65401 atgataagat tcggctacag gttcaaaggc ttccagattt tttaagaatg tctggtgcag
    65461 cgttttccct ttcgcaaacg catcgtcttt ataattttcc tgggtgtaat atttatccat
    65521 ttcgttaatg gtattaccca acgccacggc agcatcgata taactcaccg ccacgccatc
    65581 aattggttgc agcgcaggcg ttaacgccac agcacttttt acgccttttt cacactcagc
    65641 gaggttggat tcactaatgc cgtagatgcc ataaacagta cgctcttcac cggtaggccc
    65701 ctgtttaaaa tctttcagcc agtcagcata acgcgccaga ctgcgctgta ccgggatctg
    65761 taacttgttg tagcacttga tatagacatc cattttttcg tcgaaaaccg cttgttcatc
    65821 gaccaccggt tgcgcaggtg tttcaggttt agcttctgct tttgcaacgg gagcttcagt
    65881 aggtttcgct tcaggagctg gtgcggcagg ttgactattg gcaggcggga gcgtttctgt
    65941 ttcggctttt ttatcatcac aacccgtcag accgagggac atgatggcga ctataatagc
    66001 ggaagataat aaattcc
//
LOCUS       DBBGLQ010000023        65060 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.023, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000023 DBBGLQ010000000
VERSION     DBBGLQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.023"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..443
                     /gene="yhaJ"
                     /locus_tag="ACIYSB_001828"
     CDS             <1..443
                     /gene="yhaJ"
                     /locus_tag="ACIYSB_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGC5428904.1"
                     /translation="DMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVK
                     YRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIA
                     EGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK"
     gene            complement(494..850)
                     /gene="yhaI"
                     /locus_tag="ACIYSB_001829"
     CDS             complement(494..850)
                     /gene="yhaI"
                     /locus_tag="ACIYSB_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGC5428905.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(1092..1457)
                     /gene="yhaH"
                     /locus_tag="ACIYSB_001830"
     CDS             complement(1092..1457)
                     /gene="yhaH"
                     /locus_tag="ACIYSB_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGC5428906.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(1751..2737)
                     /gene="yqjG"
                     /locus_tag="ACIYSB_001831"
     CDS             complement(1751..2737)
                     /gene="yqjG"
                     /locus_tag="ACIYSB_001831"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGC5428907.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQNEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQEAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(2807..3199)
                     /gene="yqjF"
                     /locus_tag="ACIYSB_001832"
     CDS             complement(2807..3199)
                     /gene="yqjF"
                     /locus_tag="ACIYSB_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGC5428908.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(3385..3684)
                     /gene="yqjK"
                     /locus_tag="ACIYSB_001833"
     CDS             complement(3385..3684)
                     /gene="yqjK"
                     /locus_tag="ACIYSB_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGC5428909.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLETTGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(3674..4078)
                     /gene="yqjE"
                     /locus_tag="ACIYSB_001834"
     CDS             complement(3674..4078)
                     /gene="yqjE"
                     /locus_tag="ACIYSB_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGC5428910.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEDSREQ"
     gene            complement(4081..4386)
                     /gene="yqjD"
                     /locus_tag="ACIYSB_001835"
     CDS             complement(4081..4386)
                     /gene="yqjD"
                     /locus_tag="ACIYSB_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGC5428911.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(4424..4792)
                     /gene="yqjC"
                     /locus_tag="ACIYSB_001836"
     CDS             complement(4424..4792)
                     /gene="yqjC"
                     /locus_tag="ACIYSB_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGC5428912.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(4939..5322)
                     /gene="mzrA"
                     /locus_tag="ACIYSB_001837"
     CDS             complement(4939..5322)
                     /gene="mzrA"
                     /locus_tag="ACIYSB_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGC5428913.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDKTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(5326..5988)
                     /gene="yqjA"
                     /locus_tag="ACIYSB_001838"
     CDS             complement(5326..5988)
                     /gene="yqjA"
                     /locus_tag="ACIYSB_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGC5428914.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(6010..6093)
                     /gene="yqiM"
                     /locus_tag="ACIYSB_001839"
     CDS             complement(6010..6093)
                     /gene="yqiM"
                     /locus_tag="ACIYSB_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGC5428915.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(6333..7109)
                     /gene="exuR"
                     /locus_tag="ACIYSB_001840"
     CDS             complement(6333..7109)
                     /gene="exuR"
                     /locus_tag="ACIYSB_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGC5428916.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(7239..8537)
                     /gene="exuT"
                     /locus_tag="ACIYSB_001841"
     CDS             complement(7239..8537)
                     /gene="exuT"
                     /locus_tag="ACIYSB_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGC5428917.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            9020..10432
                     /gene="uxaC"
                     /locus_tag="ACIYSB_001842"
     CDS             9020..10432
                     /gene="uxaC"
                     /locus_tag="ACIYSB_001842"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGC5428918.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            10447..11934
                     /gene="uxaA"
                     /locus_tag="ACIYSB_001843"
     CDS             10447..11934
                     /gene="uxaA"
                     /locus_tag="ACIYSB_001843"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGC5428919.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGVHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            12017..12568
                     /gene="ygjV"
                     /locus_tag="ACIYSB_001844"
     CDS             12017..12568
                     /gene="ygjV"
                     /locus_tag="ACIYSB_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGC5428920.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(12573..13817)
                     /gene="sstT"
                     /locus_tag="ACIYSB_001845"
     CDS             complement(12573..13817)
                     /gene="sstT"
                     /locus_tag="ACIYSB_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGC5428921.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(14216..15181)
                     /gene="alx"
                     /locus_tag="ACIYSB_001846"
     CDS             complement(14216..15181)
                     /gene="alx"
                     /locus_tag="ACIYSB_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGC5428922.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(15464..16450)
                     /gene="ygjR"
                     /locus_tag="ACIYSB_001847"
     CDS             complement(15464..16450)
                     /gene="ygjR"
                     /locus_tag="ACIYSB_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGC5428923.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(16529..17212)
                     /gene="ygjQ"
                     /locus_tag="ACIYSB_001848"
     CDS             complement(16529..17212)
                     /gene="ygjQ"
                     /locus_tag="ACIYSB_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGC5428924.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALITKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHVFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(17289..17792)
                     /gene="ygjP"
                     /locus_tag="ACIYSB_001849"
     CDS             complement(17289..17792)
                     /gene="ygjP"
                     /locus_tag="ACIYSB_001849"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGC5428925.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17877..19013
                     /gene="rlmG"
                     /locus_tag="ACIYSB_001850"
     CDS             17877..19013
                     /gene="rlmG"
                     /locus_tag="ACIYSB_001850"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGC5428926.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPQLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            19297..19611
                     /gene="higB"
                     /locus_tag="ACIYSB_001851"
     CDS             19297..19611
                     /gene="higB"
                     /locus_tag="ACIYSB_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HGC5428927.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            19608..20024
                     /gene="higA"
                     /locus_tag="ACIYSB_001852"
     CDS             19608..20024
                     /gene="higA"
                     /locus_tag="ACIYSB_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGC5428928.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(20069..22087)
                     /gene="fadH"
                     /locus_tag="ACIYSB_001853"
     CDS             complement(20069..22087)
                     /gene="fadH"
                     /locus_tag="ACIYSB_001853"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGC5428929.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(22513..24864)
                     /gene="ygjK"
                     /locus_tag="ACIYSB_001854"
     CDS             complement(22513..24864)
                     /gene="ygjK"
                     /locus_tag="ACIYSB_001854"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGC5428930.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(24881..25951)
                     /gene="ygjJ"
                     /locus_tag="ACIYSB_001855"
     CDS             complement(24881..25951)
                     /gene="ygjJ"
                     /locus_tag="ACIYSB_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGC5428931.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(26085..27517)
                     /gene="ygjI"
                     /locus_tag="ACIYSB_001856"
                     /pseudo
     CDS             complement(26085..27517)
                     /gene="ygjI"
                     /locus_tag="ACIYSB_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(27580..28029)
                     /gene="ebgC"
                     /locus_tag="ACIYSB_001857"
     CDS             complement(27580..28029)
                     /gene="ebgC"
                     /locus_tag="ACIYSB_001857"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGC5428932.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(28026..31118)
                     /gene="ebgA"
                     /locus_tag="ACIYSB_001858"
     CDS             complement(28026..31118)
                     /gene="ebgA"
                     /locus_tag="ACIYSB_001858"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGC5428933.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPIVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(31302..32285)
                     /gene="ebgR"
                     /locus_tag="ACIYSB_001859"
     CDS             complement(31302..32285)
                     /gene="ebgR"
                     /locus_tag="ACIYSB_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGC5428934.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            32504..32836
                     /gene="ygjH"
                     /locus_tag="ACIYSB_001860"
     CDS             32504..32836
                     /gene="ygjH"
                     /locus_tag="ACIYSB_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGC5428935.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGQKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(32878..34257)
                     /gene="ygjG"
                     /locus_tag="ACIYSB_001861"
     CDS             complement(32878..34257)
                     /gene="ygjG"
                     /locus_tag="ACIYSB_001861"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGC5428936.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            34675..36195
                     /gene="aer"
                     /locus_tag="ACIYSB_001862"
     CDS             34675..36195
                     /gene="aer"
                     /locus_tag="ACIYSB_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGC5428937.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVAHQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAESVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(36349..36972)
                     /gene="nfeR"
                     /locus_tag="ACIYSB_001863"
                     /gene_synonym="yqjI"
     CDS             complement(36349..36972)
                     /gene="nfeR"
                     /locus_tag="ACIYSB_001863"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGC5428938.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            37260..38024
                     /gene="nfeF"
                     /locus_tag="ACIYSB_001864"
                     /gene_synonym="yqjH"
     CDS             37260..38024
                     /gene="nfeF"
                     /locus_tag="ACIYSB_001864"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGC5428939.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(38078..38153)
                     /locus_tag="ACIYSB_001865"
     tRNA            complement(38078..38153)
                     /locus_tag="ACIYSB_001865"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38118..38120),aa:Ile,seq:cat)
     gene            38278..38784
                     /gene="mug"
                     /locus_tag="ACIYSB_001866"
     CDS             38278..38784
                     /gene="mug"
                     /locus_tag="ACIYSB_001866"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGC5428940.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(38863..40704)
                     /gene="rpoD"
                     /locus_tag="ACIYSB_001867"
     CDS             complement(38863..40704)
                     /gene="rpoD"
                     /locus_tag="ACIYSB_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGC5428941.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(40899..42644)
                     /gene="dnaG"
                     /locus_tag="ACIYSB_001868"
     CDS             complement(40899..42644)
                     /gene="dnaG"
                     /locus_tag="ACIYSB_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGC5428942.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(42755..42970)
                     /gene="rpsU"
                     /locus_tag="ACIYSB_001869"
     CDS             complement(42755..42970)
                     /gene="rpsU"
                     /locus_tag="ACIYSB_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGC5428943.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            43208..44221
                     /gene="tsaD"
                     /locus_tag="ACIYSB_001870"
     CDS             43208..44221
                     /gene="tsaD"
                     /locus_tag="ACIYSB_001870"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGC5428944.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(44264..45727)
                     /gene="ttdT"
                     /locus_tag="ACIYSB_001871"
     CDS             complement(44264..45727)
                     /gene="ttdT"
                     /locus_tag="ACIYSB_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGC5428945.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSSDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(45775..46380)
                     /gene="ttdB"
                     /locus_tag="ACIYSB_001872"
     CDS             complement(45775..46380)
                     /gene="ttdB"
                     /locus_tag="ACIYSB_001872"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGC5428946.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(46377..47288)
                     /gene="ttdA"
                     /locus_tag="ACIYSB_001873"
     CDS             complement(46377..47288)
                     /gene="ttdA"
                     /locus_tag="ACIYSB_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGC5428947.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            47495..48427
                     /gene="ttdR"
                     /locus_tag="ACIYSB_001874"
                     /gene_synonym="dan"
     CDS             47495..48427
                     /gene="ttdR"
                     /locus_tag="ACIYSB_001874"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGC5428948.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(48440..49057)
                     /gene="plsY"
                     /locus_tag="ACIYSB_001875"
     CDS             complement(48440..49057)
                     /gene="plsY"
                     /locus_tag="ACIYSB_001875"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGC5428949.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            49162..49530
                     /gene="folB"
                     /locus_tag="ACIYSB_001876"
     CDS             49162..49530
                     /gene="folB"
                     /locus_tag="ACIYSB_001876"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGC5428950.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            49620..50441
                     /gene="bacA"
                     /locus_tag="ACIYSB_001877"
     CDS             49620..50441
                     /gene="bacA"
                     /locus_tag="ACIYSB_001877"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGC5428951.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(50622..51860)
                     /gene="cca"
                     /locus_tag="ACIYSB_001878"
     CDS             complement(50622..51860)
                     /gene="cca"
                     /locus_tag="ACIYSB_001878"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGC5428952.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(51924..52544)
                     /gene="ygiM"
                     /locus_tag="ACIYSB_001879"
     CDS             complement(51924..52544)
                     /gene="ygiM"
                     /locus_tag="ACIYSB_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGC5428953.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            52786..54087
                     /gene="ygiF"
                     /locus_tag="ACIYSB_001880"
     CDS             52786..54087
                     /gene="ygiF"
                     /locus_tag="ACIYSB_001880"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGC5428954.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            54110..56950
                     /gene="glnE"
                     /locus_tag="ACIYSB_001881"
     CDS             54110..56950
                     /gene="glnE"
                     /locus_tag="ACIYSB_001881"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGC5428955.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALCNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            56998..58431
                     /gene="hldE"
                     /locus_tag="ACIYSB_001882"
     CDS             56998..58431
                     /gene="hldE"
                     /locus_tag="ACIYSB_001882"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGC5428956.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(58551..58610)
                     /gene="ibsE"
                     /locus_tag="ACIYSB_001883"
     CDS             complement(58551..58610)
                     /gene="ibsE"
                     /locus_tag="ACIYSB_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGC5428957.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(58926..58985)
                     /locus_tag="ACIYSB_001884"
     CDS             complement(58926..58985)
                     /locus_tag="ACIYSB_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGC5428958.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(59226..60887)
                     /gene="yqiK"
                     /locus_tag="ACIYSB_001885"
     CDS             complement(59226..60887)
                     /gene="yqiK"
                     /locus_tag="ACIYSB_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGC5428959.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEVVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVEEKAE"
     gene            complement(60914..61543)
                     /gene="yqiJ"
                     /locus_tag="ACIYSB_001886"
     CDS             complement(60914..61543)
                     /gene="yqiJ"
                     /locus_tag="ACIYSB_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGC5428960.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            61812..62012
                     /gene="glgS"
                     /locus_tag="ACIYSB_001887"
     CDS             61812..62012
                     /gene="glgS"
                     /locus_tag="ACIYSB_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGC5428961.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(62055..63119)
                     /gene="yqiI"
                     /locus_tag="ACIYSB_001888"
     CDS             complement(62055..63119)
                     /gene="yqiI"
                     /locus_tag="ACIYSB_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5428962.1"
                     /translation="MRYLLIVITFFMGFSSLPAWAMDCYAEHEGGNTVVIGYVPRISI
                     PSDGKKGDKIWQSSEYFMNVFCNNALPGPSPGEEYPSAWANIMMLLASGQDFYNQNSY
                     TFGVTYNGVDYDSTSPLPIAAPVCIDIKGAGTFGNGYKKPAVCSGGPEPQLSVTFPVR
                     VQLYIKLAKNANKVNKKLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVDLEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDIGNGLLLKIFHNDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(63121..63869)
                     /gene="yqiH"
                     /locus_tag="ACIYSB_001889"
                     /pseudo
     CDS             complement(63121..63869)
                     /gene="yqiH"
                     /locus_tag="ACIYSB_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(63885..>65060)
                     /locus_tag="ACIYSB_001890"
     CDS             complement(63885..>65060)
                     /locus_tag="ACIYSB_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGC5428963.1"
                     /translation="YRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNY
                     QNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVST
                     SLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNEISHTASWYDSSDRNNS
                     WSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATR
                     HGAAFHDYSGSADSRFMIDADGTEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDT
                     RNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIIGVIRLADGSHPPLGISVKDET
                     SHKELGLVADGGFVYLNGIQDDNKLALRWGDKSCFIQPPNSSNLTTGTAILPCISQN"
BASE COUNT        15691 a        16521 c        17067 g        15781 t
ORIGIN      
        1 cggatatgca ttttcgttcc tcgtcggaga tcaactcgcg caagctctat acgttaatga
       61 acgtctacgt tgccgcgcct gatcacccga ttcatcagga gccggaaccg ttatctgaag
      121 tgacgcgtgt gaaatatcgt ggaattgcgg tggcggatac cgctcgtgag cgcccggtgt
      181 tgaccgtaca gctgctggac aaacagccgc gcttaacggt gagcacgatt gaagataaac
      241 gtcaggcatt actggcgggg cttggcgtgg cgacgatgcc gtatcctatg gtcgaaaaag
      301 atattgcgga agggcggttg cgtgtcgtca gcccggaatc gaccagcgag atcgatatta
      361 ttatggcctg gcgtcgtgac agtatggggg aagcgaaatc ctggtgtctg cgggaaattc
      421 ccaaactttt taacggaaaa taatatttat tattgttaat aaaaaggggc gaaatatcgc
      481 cccaattcta agattaattt gaaccaaact tcggatcgtt tccgtaacga ttgctgccag
      541 aagtaccttc tgtgcagaaa aaaaccagaa gcacgagcca gccaataaac ggaacaaaaa
      601 agagcaaggc ccaggcacct gaacggtcgg tatcgtgtaa gcgacggata gcaagcgcaa
      661 ggacgggaag gaaggttgcc agcaaataaa gcatagacag atatggaagc tccagaccta
      721 aaatcaattg aatgacattg ataatagcgc cgacgatggc gttaatcaga gtaaacatcc
      781 agtactcttt acgacgtgcg cgaccagaga aaccaacata attttttaat acggacaaat
      841 accactgcat aaaaacatcc ctttaattaa aagtgacgaa agtctattcc gggtattgtg
      901 taaatgctat tcgtatcttt ctttttcgga tgttataaag gttttgatat ttaagataaa
      961 tacatggtta ttttttatag gtaatttata taacgacatt gaaatatttt aaataaacat
     1021 tagtaaatta ttcattcatc aacataatga taaaagccgg atgagtcaga catccggcga
     1081 gcagatttgt tttaaggttc cagttttgga tctggtccaa agcggttttc gcctggcgta
     1141 cccgcctggc agttaaagac gatgataata agccagccga taaacgggat taagaatagc
     1201 agtgcccacc acgccgagcg gtcggtgtcg tgcaggcggc ggaactgaac cgcccaccac
     1261 ggtaaaaaca ccagaatacc gtagattgtc gtcaggatgc cttcgccacc agcgcgttgc
     1321 cagcctaaca ttttatccag cagccccagc acgaacgtaa agatgatgtt gaccagaata
     1381 aacatccagt actctttgcg ccgcgcacga ccccggaaac cgacataatt ttttagtact
     1441 ttcagatacc agtccatttt tgcctcaatt ttgctcaatc acttgttttt aaagcagcaa
     1501 attaagaata gacgtgaatg aaatggcagt aaatattcgt tcaaggaatt aataaagtgc
     1561 cgtgaaggcg cgctaagact gatgacaagc gcaaaatcgc cagatgcgct acgcttatct
     1621 ggtctgcgcg ggttatgcaa tctattgaat gtacaatgtt ttgtatgtca gataaggcgt
     1681 tcatgccaca tccgacatga acaacatgca cttagctaat tatcagaggc acctcgtaag
     1741 gtgccttttt ttaaccgaag cgaacatctc gtccatgcgg ttcatcgaga tcctgccacg
     1801 gaccaattga aataatcccc gtagggttga tggtcttatg gctgcggaag taatgattac
     1861 ggatatgatc gaaattgact gtttcggcga tccccggcat ctggtagata tcgcgcagaa
     1921 aaccgtacag attcaggtaa tcgctgatgc ggtgcttatc acacttgaag tgggtcacat
     1981 acaccggatc aaaacgcacc agcgtagtcc acaggcgaat atcggcttcg gttagctggt
     2041 tgccggtcag gtaacgatgc tggcctaaaa tctgctccag tcgtgccagc gattcaaaca
     2101 ctttcgccac cgcctcgtcg taagcttcct ggctggtggc aaaaccagct ttatacacgc
     2161 cgttgttaac ggtgtcataa atccagccgt taagttcgtc aatttttgtt tgcagggctg
     2221 gagggtagta atcacccgct ttcgcgccca gcgcatcaaa cgcggtattg aacatgcgga
     2281 tgatttctgc tgattcgttg ctgacgatgg tgtggttctt tttgtcccac agcacgggaa
     2341 cagtaactcg tccgctgtag tgtggatcgg cgtggagata aagctgatac agaaattcat
     2401 tttgatagag cgtgtctccg gttgctcccg gaaaactgtc atcaaaggtc caaccgtttt
     2461 ccagcatcag cgggttcact acggaaacgg aaataaacgg ttccagtcct ttgagtttgc
     2521 gcatgatcag cgtgcggtgc gcccacgggc aggcgagtga aacatagaga tgataacgat
     2581 ctttctctgc gataaaaccg cctgtgccag tggggccagg agcgccatcg gcagtgagcc
     2641 agttacgaaa tgcggaagct gaacgttgaa atttaccgcc ggtagatttg gtatcgtacc
     2701 aggtgtcatg ccagacgccg tcaatcagtt gacccatttt tctctcctcc aatagcaaaa
     2761 acgaggagct tcctcctcgt tttttatcgt taagtatagc gcgtgcttac cactttttat
     2821 tcagcaggcg gtcgatgcta tacgcgcccg gaccggtaat tgccagcagc aggaatccgc
     2881 cagaaattgt caggtttttc atgaacatca gcgagttgac gccttcagca aagttgctgt
     2941 gaaataaaaa tgccgtcagc agcgtaaagc ccgcagtaaa cagggctgtg gtgcgagtca
     3001 ggaaaccgaa caggattgcc agaccaccac caaactcaag cagaatcacc agcggcagca
     3061 taaaacccgg gacgcccatt gcttccatat attgttgggt acccgcgtag ccagtaattt
     3121 ttccccagcc agcggtaata aacagaatcg gcattaaaat gcgcgctacc agtacaccaa
     3181 catcttctaa ttttttcatc atactctcca ctgcgccact caggctgctg attgtttttt
     3241 tgtgcaatgg cgcggtatta gcgtcgttgc tgtcgatgga gagaatcata aacgtggtga
     3301 atgatgattg ttagcaagga aaactgtcaa aaatcttcaa aaaatttgag ggataaggcc
     3361 ggaatggctc cggccagagg gaagttaacc gcgaagctgt tgctgcttga gggtcgtttt
     3421 aaccagacgc caggcgctcc atacgccaaa accgcgtctg gcccagcgga ccagcatatt
     3481 aggatggcga atcgtccaga tcgccatcac gctactgcca accagcgccc aggagcgcag
     3541 acttagcagc atattccagc gacgatcgta agcgcctgtt gtctccagcc attcacgacg
     3601 actggcggaa agatccagcc gttgttgctg gatctggcta agcagttgcg ccttacgtcg
     3661 ttcacgttcg actttactgc tcacgggaat cctcctcgag cagctgccga tcgtttgcta
     3721 actcatggcg tgtatggcgc agcaacgtag acttacgcga tttacgtagc gtccagatac
     3781 cgccaatcag tgccagtagc agcaacacca cggtggtggc aatcatcgca ttcaggcgat
     3841 attgcgggtc aaccgcccaa ataattagca ccatcaggct cataagacca aatgcagcga
     3901 aaagcatcgt caggcccagc atcagtaaaa gttgaaagag attcgctttt tcctcttcca
     3961 gctccaccac cgccagccgc agacgtgtct ctaccatttc aaccatgata gaaacaattc
     4021 gctgcccgat gcccagaacg cttttaccgg gcccttgtgc gtgatgagtg tccgccataa
     4081 ttaacgacgc gacagcagaa cgccgagcac tacaccgatt gcagcgccaa tgcccacgcc
     4141 cgtccacgga ttttcgcgca catactcatc ggcacgcgcc gccgcgacac gggtttgttt
     4201 ggcaatggca tcaccggttt cacccaggcg ataacggctc tgtttcagtg cctgctccgc
     4261 tttgctacga atcttactca actcttcttt cgacttctcg ccagatgagc taagcacctc
     4321 ttccagcgta tcggaaaggg atttcaactc agcacgcaga tgttccgtag tgtgttcttt
     4381 cgacatagtt ttctccaggt gagtaagtag tgactattgt gagttagtag tcgcgcgctt
     4441 ccagcttttt cagctcttcc tgcgcttctg ccagtttccg ttcgcgtttg gcaatcttat
     4501 cggcatcgcc tttttgcttc gcctcggcta aatcttgctg gcgttccgcc acctcatctt
     4561 tctgctttgc gattttcttc tgatgatcgg cacgcagctg gctatctgaa cagttggccc
     4621 ggacttcact cagggcttta ttcagaccgt caatacgatt ctggttttgg tgtttttcgg
     4681 catagctgat ctccttaagg atattttgct ccttttcctg acacagggta gtggcataac
     4741 taccggcact aagagcaaag agagaaacag ctaaagcgat gcggtatttc attcttgaac
     4801 cttccattgt gattcggacc tattacaaag gaacgattgt ccagacaacg aagcggtagt
     4861 gttaaccacc gcctcattaa tcatagacag ttacggtgaa atcaccaaag tgagtgatat
     4921 gtttcggata acaggaagtt atccgaagcg atgagagtta tcccgtaacc gggtcagcca
     4981 ctgcatagcc tgactgttat tgtcctgctg cgcaatgatg tagccatggg gcaatgtttt
     5041 gtcgaggacc gcttttgcgg cggcgctctg gtcgctggag tcaaatgtaa ttaacagagt
     5101 gtcgtttttg ggggtgatac ttttgaaagg aatgccatga gcgtcaaggt gatgccagat
     5161 tgaaaaaccg tctggcatcg ttgtgccttg atgaacggca cgaatcgcca gcgtagactc
     5221 ttgctggcga accgcggacc aggccagcaa cagcgtgccg accagaagta aaacagcgcc
     5281 ggaccaggct agctggcgaa gcgacatgcg aggtatttgc atcccttacc cccgatttcc
     5341 atatttcttt ttccataaca cgaccagaga acctgccagg ccaaacacca gcagcaccac
     5401 cgggagcagc atcaggcatg acatcagctg gtcctcgtac tttaaaaata ccggcgtttt
     5461 gccgagcatg taacccagag ttgtcaggat caatacccac agcagaccgc tcatccagtt
     5521 gaaaaactga aagcgcgcgt tattcagccc tgataacccg gcaatcgtcg gcagcagtgt
     5581 tctgacaaac gcaataaagc gaccaattaa cagcgccgat aaaccgtgtt tatgaaaaag
     5641 atggtgtgcg cgttgatgat aatgcgcggg taaatgagat agccagtttt gtacggtgcg
     5701 ggtattgccc agccatcgcc cctgaatata gctgacccag cagccgaggc tggcggcaac
     5761 ggtcagcagc agaatcgttt gcggatagcc catcgcgcct ttcgcaatca acacgccgac
     5821 caataccagt aaactgtcgc ccggtaaaaa ggccgccgga agcaagccgt tttcaaggaa
     5881 caaaattaca aacaagacaa aatacaacat gccaatcatc gatggattgg ccagggtttc
     5941 aaaatcctgc gcccacaggg cttgcagcaa ttgggtcaaa agttccattc attattcctg
     6001 gtgaatcggt taacgccacg ctggttttaa aagatgcact accgcaatgt tattgctgtt
     6061 ttggtatgtt ctgcgggttt gatacatcaa cacagcctga actattccct gttaaatcgt
     6121 gagataagaa agcattcaaa aacataaatt caaattgaat ctaattgtaa caaaggtcaa
     6181 cgttgttcgt tatcaaaatt acatgtccgt ggagtctgat tttgctgaat gcaggggagg
     6241 gtggcgagag gatttacaca ggctgacact ttatactttt cgcttaccga cgagacaaac
     6301 aggcgcattg cagcgcctgt cggacaggaa tttcatttac tgccgctggt ggctgtatcg
     6361 agatgcacca ccgggttttc ggcgaataga tagcggtcgg cattgaactc gaagtcgtcg
     6421 ctggtttcgt taaataacat gatcttggtg ttttccagat gctgccacat tgccagctta
     6481 gcagcatgag gatctttgcg aatcagcgcc ttgaggattt gatcgtggtc atcacaccag
     6541 ttatcgacgg tacgggaatc aatgtgttcg tgcagttttt tccagtacgg gttatgacta
     6601 cgctgggtcc acattttttc cacgatagcc gccagggcgg agttctgcgt cgccagagcg
     6661 acctgaatgt ggaactgcaa atcccactcg gaatcacgga agcattgttc gccgcgcgcc
     6721 tgttcctgaa tggccatcag tttcatgatg tcctgtttcg ttacctgagt tgccgcgaat
     6781 tcggcaatat tactttcgat gagctggcga gcctgaagca actcaaacgg accgtaattg
     6841 gcgaattcca tattattgtc agccgcctgc tgatggcgcg gctggttgga aaccacatga
     6901 atacctgaac ctttacgcac ttcgacatag ccttcaactt ccagcatgat gatggcttca
     6961 cgaactaccg tacggctgac gttcttttca tcggcaataa agcgttctgc aggcagttta
     7021 tcacccacca gatagacgcc ctgttcgatg cgctctttca ggtcagcggc aagttgttga
     7081 tacaaacggc gtggttcagt gatttccata tgcgctccag gcatgatacg gcagactata
     7141 tttgttatac cacttttgcg agtttagctc tactcgcagt gatgaaaaag ccgcctgaga
     7201 aggcggcttt gaagggaaac taaaacttac acgaaacgtt aatgttgcgg tgcgggatcg
     7261 ttatgtgttt cctgcgccac ctcaatcgcc ggtttgttct gcaacacggt ccagataacc
     7321 agcgcaccta acaggtcgaa cactgccaga actgcgaaca gcgggctgaa gccgatggtg
     7381 tcagccagtg caccgacaac cagcgcaaac agcgtacttg ccagccatgc ggacatcccg
     7441 gttaaaccgt ttgccgttgc cacttcgtta cgaccaaaca catcggaaga gagcgtaatc
     7501 agcgcgccag acagtgcctg gtgggcaaaa ccaccgatac acagcagcat aattgcgaca
     7561 tacgggttgg tgaacaggcc gatcataccc gggccaatca tcagcactgc acccagcgtt
     7621 acgaccatct tacgggaaac gatcaggttc acaccaaacc aacgctggaa cagcggcggc
     7681 aggtaaccac cgaggataca accgaggtca gcaaacagca tcggcatcca ggcgaacatc
     7741 gcgatctctt tcaggttaaa gccgtaaact ttaaacatga acagcgggat ccacgcgtta
     7801 aaagtacccc aggccggttc tgccaggaaa cgcggcagcg cgatacccca gaactgacgg
     7861 ttacgcagga tctgaccaac ggacattttc ttcgccgtgc tcacctggtg ctgggcttcc
     7921 tgaccattaa taatatagtc gcgttcttca tcggtcagat gcttctggtc gcgcggatgt
     7981 ttatagaaaa tcagccatgc catcgcccag ataaagctca atgcaccgga gatgataaat
     8041 gccatctgcc agctgtgcat tacgattgcc cataccacca gcggcggcgc aatcatcgca
     8101 ccaatcgaag aacctacgtt aaagtagcct actgcgatgg aacgctcttt cgccgggaac
     8161 cattcggagc tggctttcag acccgccggg atcatcgctg cttccgcggc accgaccgca
     8221 ccacgagcaa cagccaggcc accccagcta cctgccagcg cggttgcacc acagaacacg
     8281 gcccacagca cagcaaacat tgcataaccg attttcgtac ccagcacatc cagtacataa
     8341 cctgctaccg gttgcatgac cgtataagca gcagaatagg ctgcgatgat ataggaatac
     8401 tgttgggtgg agatgtttaa ctcttccatc agagttggcg cagctgccgc cacagtgtta
     8461 cgcgtcaggt aaccaagcac ggtgccgagc gtcaccagtg cgatcatata ccaacgtaac
     8521 cctttaattt tacgcatgta aaacctcatc gttttgttat atccgcagga agcgcggcca
     8581 tcatcccgcc agaggctgaa gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac
     8641 gccccgaacg ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat
     8701 tcatctaaca aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc
     8761 agaaagataa cttgtcatac aactttaaaa ggtgagagcc atcacaaatg tgggaatatt
     8821 tgtagggaca ttacctgacg acagcaaggc cagtactggc gcggcctgca gcgagattta
     8881 ccactttgag agtaattttt ttaactacgt ttattgatct aactcacgaa aatatcttcg
     8941 gactctggaa attggtgtga taactttgtc agcatcgcac cataagcaag ctagctcact
     9001 cgttgagagg aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga
     9061 atttgcccgc cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg
     9121 ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg
     9181 gctgaaaggc gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ccgagcgtct
     9241 gtgtaccggt gatgcgtctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca
     9301 cactatcggc aacccgttat accactggac gcacctcgaa ctgcgccgtc cgtttggtat
     9361 cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact
     9421 gctggcgcag gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt
     9481 cggcaccacc gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg
     9541 ctctttcacc atcaaagtgc tgccgagctg gcgtccggac aaagccttca acatcgaaca
     9601 ggcgaccttt aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg
     9661 ctttgctgac ctgcaaactg ccctgaccaa acgtctggat cacttcgccg ctcacggctg
     9721 taaagtgtct gaccacgcgc tggatgtggt gatgtttgct gaagcgaacg aagcggaact
     9781 ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg agcgagcacg aagtggcgca
     9841 gttcaaaact gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca
     9901 gcagtaccac attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc
     9961 ggatgtaggc tttgactcca tcaacgaccg tccgatggcg gaagagctgt ctaagttgct
    10021 gagcaagcag aatgaagaaa acctgctgcc gaaaaccatt ctgtactgcc tgaacccgcg
    10081 cgataacgaa gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa
    10141 aatgcagttc ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat
    10201 gacccaactg gcgcagctcg gtctgctgag ccgctttgtc ggtatgctga ctgacagccg
    10261 tagcttcctg tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg
    10321 tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa
    10381 aaatatttgc tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg
    10441 gttgatatgc aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat
    10501 ttggctgaag gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt
    10561 gctcgtggac ataaatttgc gttaacggat atcgcaaaag gggccaatgt catcaaatat
    10621 ggcctgccga ttggttatgc attggcggat attgcggcag gtgttcacgt tcacgcccac
    10681 aatacgcgca cgaatctgag cgatctggat cagtatcgct atcaacctga ttttcaggat
    10741 ctgcctgcgc aagcggcaga tcgtgaagtg cagatctatc gtcgcgctaa cggcgatgtc
    10801 ggggtgcgta atgagctgtg gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg
    10861 cagatccaga accgttttct gaaagagacc aacaacgccg aaggtactga cggcgtgttc
    10921 ctcttcagcc acacctacgg ctgctcacag ctgggcgacg atcacattaa tacccgcacc
    10981 atgctgcaaa acatggtgcg ccacccgaac gcaggcgcag tgctggtgat tggtctgggc
    11041 tgtgaaaaca accaggttgc cgcattccgt gaaacgctgg gcgatatcga tcctgaacgc
    11101 gttcatttca tgatctgcca acagcaggat gatgagatcg aagccggaat cgagcatttg
    11161 catcagctgt ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg
    11221 aagtttggtc tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg
    11281 ctggggcgtt tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg
    11341 ccggagatgt ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt
    11401 gaaaaactgg tcaccatggt caacgacttc aaacagtact ttattgccca tgatcagccg
    11461 atctatgaaa acccatcgcc ggggaacaaa gcgggcggta tcaccacgct ggaagacaaa
    11521 tcacttggct gtacccagaa agcgggttcc agcgtcgtgg ttgacgtgct gcgttacggc
    11581 gagcgtctga aaacgccagg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg
    11641 accagcgccc tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgtggcacg
    11701 ccgtatggtg gatttgtgcc gacggtgaaa atcgccacca acagtgaact ggcggcgaag
    11761 aaaaaacact ggatcgactt tgacgcgggt cagttgatcc acggtaaagc gatgccgcag
    11821 ttgctggaag aatttatcga caccatcgtt gagtttgcca acggtaagca aacctgtaac
    11881 gagcgtaacg acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga
    11941 aatgagttgt taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac
    12001 ctgctaagga tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt
    12061 tctgattggt atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc
    12121 ggtctatagc gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc
    12181 cagtgccatc cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt
    12241 aatggcgatt tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt
    12301 cgaactattg ccggttatcg gcacgattgt cagtacctgg gcgctattcc gctgtaaagg
    12361 gctgaccatg cgctgcgtaa tgtggttttc aacgtgttgc tgggtgattc acaacttctg
    12421 ggcggggtcg ataggcggca cgatgattga gggtagtttt ctgcttatga atggcctgaa
    12481 tatcattcgt ttctggcgga tgcagaaaag gggaattgat ccgtttaaag ttgagaaaac
    12541 cccttccgcc gtagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga
    12601 cgatcgtctt ctgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc
    12661 gcggtttcgc aagagtcctg caatacgccg atgataaagc cgacggcaac cacctgcatg
    12721 gcgatatcgt tcgaaatacc gaacatatta caggccagtg ggatcagcag cagagacccc
    12781 cccgccacgc cggatgcgcc acaggcacac agagaagcca ccacgctcaa cagcagcgcc
    12841 gtgggcagat cgaccggaat acccagcgta ttaaccgcag ccagcgtcaa cacggtaata
    12901 gtgattgctg cgcccgccat attgatggtg gctcccagcg gaatagaaac ggaataggta
    12961 tcgcgatcca gattcagctt ttcacacagc gccatattca ccggaatgtt cgctgcagag
    13021 ctgcgggtga agaaggcata cacaccgctt tcgcgcaggc acagcagcac cagcgggaac
    13081 gggttacgac gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac
    13141 atacagccaa ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg
    13201 gttgccaggg tagaagaaac cagcccaaaa ataccaatcg gtgcgaagcg aatgaccagt
    13261 ttcaccataa aggtaacggc attcgacata tcgttaacca ggtttttggt ggtctcgtta
    13321 ccgtgacgca gtgcgaagcc gaggccgatc gcccacacca gaatcccgat gtagttacct
    13381 ttcagcagcg cgtcgatggg gttggaaacc atgctcatta ccagcccgcg catcacttcg
    13441 acaatgcctg acggcggcga aatatcaccc gcgctactgg ataaatgcag ggtagaaggg
    13501 aaggcaaagc tgaagactac tgcggccaga gcagcagaga aggtgcccag tagatagagg
    13561 aacaaaatag ggcggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatc
    13621 accagcatca acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct
    13681 aacagaccaa cagcttccgc cgcgggtttt gagatccatg ccagaagaat ccccagaaca
    13741 aggccgacca ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt
    13801 gaacgttgcg tagtcatttt tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt
    13861 ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt
    13921 acagcttcgg gaagcgaaaa atgctggatt ttaaagagag gtcatatttt gaaacttaat
    13981 ggttacattt atttgacata aaaataaagc caacgtgcgg cttccagact gatgacaaca
    14041 tcaaaaaagg actcggtcag tcccctcgcc ccgtttgggg agagggttag ggtgagggga
    14101 acaggccagc atcggtgcga aaaattaccc tcaccccgac cctctccctg gaagggagag
    14161 ggagaaaacg gtgctcatgt taatgacagg gtacacggct taggcagatt aaaaattatc
    14221 caccccgctg cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca
    14281 gaatgccaaa caccacgccc agcgagacgg cgattggaat atggtagaag tcgacaatca
    14341 gcatcttgat accgataaac accagaatca ccgccaggcc atatttgagc atcgagaaac
    14401 gctctgctac gcccgccagc aggaaataca tcgcacgcag gccgaggatc gcaaacaggt
    14461 ttgaggtcag cacaatgaac gggtcagtgg tcacggcgaa gatagccgga atgctatcca
    14521 cggcgaaaat cacgtcgctc aattccacca gaatcagtac cagcatcagc ggtgtggcgt
    14581 acagcaatcc attcttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca
    14641 aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg
    14701 cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt
    14761 cgaactgcga aattagccag ctgccagtga agatcatgat ggtacgcaga acaatcgccc
    14821 cgagcacgcc atacaccagc acgcggcgtt gtaatgccgc cggaacagag aaatagctga
    14881 acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc agataacctg
    14941 tgagaaaggc cagtgcctgt ggatcggcaa cggcgcgacc ttcggtttgc accagatacc
    15001 accagaaagc ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag
    15061 cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca
    15121 gcataatggc gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca
    15181 tagaagttcc ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt
    15241 atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg
    15301 tattcctttc gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg
    15361 accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca ttcagccagt
    15421 caatggtctg ttacatacct tttacactgt tttactttgg caattatagt tttacgctat
    15481 ctgccggaaa aatcacccca gtctggcggc ggatctcggt cagcagtttg gctgtgatgc
    15541 gactgaccgc cagccctgga tgatccacca gatgctcatc caccagggta gcgaacagct
    15601 ctgcttcgta gagcatggta ttaatatgct gcggctgggt gagatcctgc atctggctgc
    15661 cacgcggcac aaagcacact ttctggcatt cagacagttt ttcaatcacc agcgatcccg
    15721 cttcgccctg aatctcgctt gccaggacag aatcactgac ctttgagtgc tgcaaggtga
    15781 cgctgaaatc accgtaatcc atcaccacca cgccgtgggc atctacacca cttgccagta
    15841 aactggcggt tgcctgcacg cttttcggct caccaaataa cgctaccgcc gacgccaggc
    15901 agtaaaagcc gatatccata attgaaccgt tagagaatgc cggattaaag gtgttgggat
    15961 tctcaccatc caggtaacgt tgataccgcg aggaatactg gcaatagttg aaaaagactt
    16021 tacgcaattt gccgactttc ggcagcgcct ggcgcagcaa atgaaagttc ggcaggcagg
    16081 cggttttaaa tgcctcaaac agcaccacct gattttcccg cgcacaggca atggcggcat
    16141 ccacttccgc cagattcgac gccagtggtt tctcgcaaat cacatgaatt ttatggctaa
    16201 ggaaaagttg tgtctgggaa aagtgcaggg cattcgggct ggcaatatac accgcgtcaa
    16261 tggcatcgct ttccgccatc gcttccagcg aggtaaacag atgctcgaca gaaaaatcat
    16321 tggcgaagtg ctgagcctgc tcaaggctgc gggaatatac ggcggttaac ttgtatttac
    16381 cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga
    16441 aacgtatcat aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt
    16501 tttcatcttc gacgtgcgca gatacgcatt attcttttgc cggacagcca tgctcactaa
    16561 acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg attttggcgt
    16621 caatcaccgc gctgacacga gcgagtttct cccgcagttg agtataaaaa acgtgcttca
    16681 tattgaggtc tggcgcgtta aacccgatag cgtcgatgcc gtactgtttt gccagccaga
    16741 tggcgcgttg gttatgaaat tcttgcgaaa taatggtgat atggttttca ccaaacacct
    16801 ttttggcacg caccaccgaa tcaagcgttg agaatccggc atagtcgcag aagatcactt
    16861 tcgccggtac gcctttagtg atcaaagcct gctgcatacc tgatgcttca tcgtaatttt
    16921 tacgtccgtt atcgccactt accagtagcc atttcacttt tccggcgtgg tacaacgctg
    16981 ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctcgccccc agcaataaac
    17041 ccacgttacg cgccggaact gcgttgacat cgctccaggt cagctgcttg ctggcgttga
    17101 ccataacgcg atcggcaata aaaatggtcg ctcctgctat aagcaacaat aaacagccaa
    17161 tcttaagagt ccggcgagaa aaacaaatac ggagaagaag gcggggctgc atggtcgtta
    17221 tccttaacga aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga
    17281 catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg
    17341 aactcaagct ggtggtactg cggctccata tggcagcaca attgatagaa cgctttgtta
    17401 tgctctttct ccttcaggtg cgccaactcg tgcaccacga tcatgcgcaa aaacggttcc
    17461 ggcgcgttgc gaaacacggt agcgacgcgg atctccgcct ttgctttcag cttgccgccc
    17521 tgcacacgcg aaacagcggt atgtaacccg agcgcgtttt tcagcacatg gatcttattg
    17581 tcatacatca ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga
    17641 gtatattgcc acagcgcttt atcggtggcg tagtcgtgtg ttcccggata acgttttgcc
    17701 agcacatcgc ccagacgctg ttcgttaatc agcgttcgca cctgggaaag tagctgctcg
    17761 ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt
    17821 atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga
    17881 gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg
    17941 ttaacccact acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca
    18001 cagaaatccg cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agttgtgcgc
    18061 tggcggaaca taagccatac agcattggcg actcatacat cagcgaactg gcgacgcgcg
    18121 agaatttacg cctcaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg
    18181 actacccgca acagccgggg gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg
    18241 aacaacaact gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg
    18301 ccaaggcccg tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccaa
    18361 ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa ttgcactttc aatgaaccgc
    18421 agctggccga tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc
    18481 acaaccatgc gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc
    18541 aacatctgcc agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta
    18601 ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc
    18661 cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc
    18721 gctgcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg
    18781 tgctctgcaa cccgccgttt caccaacaac atgcgctgac cgataacgtc gcctgggaga
    18841 tgttccacca cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc
    18901 gtcacctgga ttacttccat aaactgaaga agattttcgg caactgcacc actattgcga
    18961 cgaataataa atttgtggta ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg
    19021 agttacctgc cggatgcggc gtaaacgcct tatccggcct acgttcagca cgttactcca
    19081 ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgcccagttc tggaatcgcg
    19141 cttccataaa acaaaaaatc cctgtcacca tcgtctacat tctcttgttt agcgtttttc
    19201 tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctggc aacggatgtt
    19261 atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca
    19321 ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctggggaac
    19381 acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg
    19441 gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt
    19501 gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat
    19561 aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg
    19621 ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc accttttctg gcgggtattc
    19681 agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg ctcaacgatc
    19741 ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc
    19801 ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc
    19861 gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat
    19921 ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat
    19981 tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg
    20041 cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt gttccctggg
    20101 caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt aaatgcaccg
    20161 ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga
    20221 tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt
    20281 catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcaggg
    20341 tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcctggtttg ctggctttgc
    20401 gctggagcat cacaatctgc cgtgggctac gggggatctg cattccctgc gggcttaagc
    20461 caccagcctg ttgtaggcta ctgtcgatcc cccattcatt acagaacccg gcgatattct
    20521 ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac
    20581 aaccgatgat ggcaactttg ttgccaaccg gcgctttgtc gcgcagtaca tcgagataac
    20641 tcaatacctt cggatgatcg atcccgtcga tgggcggagt gcgcggcacg atcccactgg
    20701 cgaggatcgt ttcatcgaaa gcctgtaact gatccgccgt cacggtgtga ttgagtttta
    20761 gcgtcacgcc cgtcacttcg atcatccggc ggtaatagcg cagcgtttcg taaaactcct
    20821 ctttgccggg gatctgtttg gcgatattaa actgcccgcc aatctcgcta tgagcgtcaa
    20881 acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc
    20941 ccgcaccgac caccgccaga ttttttttct gcacggcggg aaggattggc attttggttt
    21001 cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag atttgatcga
    21061 gacaggcctg attgcagcca atacaagtgt tgatctcatc ggctcgtccc gattgcgctt
    21121 ttgacagcag ctccgcatca gcaagaaacg gtcgcgccat cgataccata tcggcatcgc
    21181 cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg gttaccagcg
    21241 gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg
    21301 gcgtggcaat ggtcggaata cgtgcttcat gccagccaat gccggtgttg ataatggtcg
    21361 cgcccgccgc ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc ccgccgtctt
    21421 cgaccaggtc gagcatcgac agtcggtaga taataatgaa gtcgttgccg acgcgttcgc
    21481 gcaccgcacg cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact
    21541 ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcggaac
    21601 ccatcacctc tacaccgtcg tatcctgcct cccgcgccag ttgcgcgcag cgggcgaaat
    21661 tgtcgatcag ttgcaggatc tcttcatggc ttaactcatg gggcacgaaa cggttgatgg
    21721 gggcctgcaa tgcggacggg gcgaccagat gcggttggta gctgtagcgc ccggtatgca
    21781 aaatttgcag ggctattttg ccgccttcct gatgtaccgc ttcggtaatg gtgcgatggt
    21841 gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat
    21901 ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa
    21961 acgctgccag ccgctcggca ccgtccgggt attcctccag cccggtgtgc attgagccca
    22021 tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt
    22081 agctcataaa attgtccagt atgtaaaata attgttatgt ggtcggatga gttctaattt
    22141 agccgtcgcc ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga
    22201 atacgcccgg tgataacgcc ggatgcgacg cttgtcgcgt tttatccagg ttacggaatt
    22261 gcacatgtag ggcggataag gcgtccacgc cgcatccgcc agtggcgcgg tgcagatgcc
    22321 ggatgcgacg cttgtcgcgt cttatccgac ctacgggaac gcacatgtag ggcggataag
    22381 gcgttcacgc cgcatccgcc agtggcgcgg tgcagttgcc ggatgcaacg cttgccgcgt
    22441 tttatccggc caggaggatt gcacatgtag ggcggataag gcgtttacgc cgcatccgcc
    22501 agcagttgca aattattgct tacggaaaaa atcgttatac aacatataca aatgcgcggc
    22561 actccaggag aaatttggtg cgccttgctg tgcgcctgtc agcgggttgt aattttcctg
    22621 aattggtcca tcggcggtta accctttggc gtgccggaag aacgtatccg ccagcttcag
    22681 ggcatcatca cgataaccgt aacgctccat ccctttcaga ccaaaccaga actgatccac
    22741 ccatacgcgc ccacgccagt agatatcagc gccaaaagcc ggattggtta acgccgccgt
    22801 tcccagcggg acaaaggtgt tgaactcttt aggatcgagc atcaccttca ccaccgcgtc
    22861 ggcattggcc tgcgttgccg caccgttaaa cagcggcgac cagccttccg gccctttacc
    22921 gcgctcaaca atcggtttgc ccgcgcagcc gttcgccagt ggtttatctt caatacgtac
    22981 gtcatagtag aactgggtag tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg
    23041 ctgtgccaac tggcgatagc gtttggcctc ttctggctta ccgaggatgg ttgccatctc
    23101 cgccagataa tggttatcgc tgtacatata gctggcctga tccaccgact cctgcaatag
    23161 cgagtagccc agcaacgttc cgtcctgact gcggttttcg gcgaatttca ccgtccagtc
    23221 gctacgtttg ccgccattag cgacatattt atccaactgt tctttgtcga taaacccaaa
    23281 gacggcggca tcatcacgcc ccgattccca cgatgcagca acctgtgccg gaatttccag
    23341 actgtcgtac tggcctttct ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc
    23401 ttctttatcg ccttttttca ccgtaaacag catctcgccg ctctcagtgt tgtgggcttt
    23461 gtcgcgggtc gcgccatatt ccggcacgcc gttgccgttg tgatcgcggt tacgtaacca
    23521 ccagtcgtga taggccacca gtttcgggta catctctgcc acccaggttt tatcttgggt
    23581 gacgttatac acttccatca ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt
    23641 ccagttgccg ccatcgccac cacgctcggg gctaaggttc cacgctatca ggtcggggac
    23701 aaagcccaca tcctgcggac gcacgctatc gccaggctgg atctgccagg agaagaccgc
    23761 gcggatattc tctttggcga tgtccggatt gaaatgcgcc atcgcaaacg cctgcttcca
    23821 ggtatcccac ggccaggtct gattgccgga gaaccagcgc ccggtcaccg acggtgtaac
    23881 ggtgttaaat ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc
    23941 tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga ttggttaacc ctttcttcag
    24001 gtattcttcc cagcgttgct gcgaggcggt gagataaaac gccggacgcg ccagaatatc
    24061 gcggatctgc atttgctctt tgctaacttc ctgagcggtc agcagatggg aataggtggt
    24121 gtagagcgtg gtcgaaccgt tgatatgcgc cttactggta aagcgattac cgttgatttc
    24181 agtctgcacc ggcagggatt tatgcacctg atattccgat tcgccggagg tcagcagatc
    24241 ccaggtggcg cgcactttgc caaaggtgac tttcaggcca tcacgggtgg cgctgatttt
    24301 gcgctgatag tcagggtatt cgccagcaat ggttttatcg gaaagcggtt tcccttcttt
    24361 cgcttccagt ttttccagca gttcgccgtc ccacaccaga tccagcggtt tattgctggt
    24421 gattttggtt tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca tttcgacttg
    24481 cacatctttt gctgtcagtt tttgcaccag cgcaccggga atactgtatg cctccagcgt
    24541 aaagtcgact ttcttgccgt cctgccagac ggtcaggcgg tcgaaattgc tggccataaa
    24601 gttgatgtac tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg tgttagggcc
    24661 gtctggcaac agatgaccat gccaggctcc gagatcgaaa aacggattaa aacgctggtg
    24721 atcgtcgtaa tcgtaatcct tcatgtactg cggcgcgcca gtacggttaa tcacgttttt
    24781 ataattgtcg gcgttagcgg catgggcgga aaaacttatc agcagagcgc aggttactgg
    24841 cgttaaaata gtttttattt tcatgaaatt acagtcctta ttaccagtag aaatattgca
    24901 tcaggaagat ttggtcgctg ggatcgtgag aggcattttt catgtagaag cggctatcaa
    24961 aagccgtggc gaaacgtccg ccgccgtatt cgtaccagat accaaactgc atgaaagagg
    25021 tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg gtggagagat
    25081 aaacgccctt taacgcttca tacatggcgc tcaccatcat cccactttca cttcccggta
    25141 cgtccagccc gtcggcgcgt ccggtatggt gccaggcgcg agcggcaaag ttaggagtta
    25201 gcaggcccgt gagatagagc tgtcctgtgc cgtcggtgat ttccagaccg ttcatccagg
    25261 tgatgccttc ggcaaggtca tactggatgt aaccgttcat cattgccgga taggtgtatt
    25321 tgtcgtcata gcgatcgtat ttgccaaaat gtagccaggc tttgctttca tcatgatgtc
    25381 cggcaggagt cgcggtgacg ctataacgaa agttgccgct taagttctgt accttcagcg
    25441 cagtcataat gtcgcgggta ttgggaatga cgtagcccag atcgggggtg aaatcgcccc
    25501 accattgcag gtcatccagc gaggaatcct gacgcagact gagcatgatt tcggtgccat
    25561 cattggtacg ataaccgcca tagaaacggt taattccgcc ctcgaaacca ccccagctat
    25621 ggtctggcac ccaggcattg ccgtcgtggt cggcctgcac ggtccagcct tcgccgtaaa
    25681 tcaggccgaa ccaggagccg tatttcatct ccaggccacc ttcaatatag gtaccgtcgc
    25741 tgtatttatg tttattctca ccctctaaat ccatatatcc accaacccct aattcgccgt
    25801 aaaaacgaaa tgaaggagaa gatgtcgagg taattgcggg tgtttttgtg tcgtcatgtt
    25861 ctgccggacg tgcttcttct cctgccgcag aaaaggcgta gcaaaacggc agagcaagta
    25921 atgctctgca tggtgcagtg attaatttca ttgtgttaat ccatgagcta aagaaaaagt
    25981 acagcctttt atcgttgtaa ttataaaaat ataaaggcag aaactatttt ccctctcctc
    26041 atccaggaag agggaaaatg cctgctcatt ttttattatt ctgcttaatc aacattgctg
    26101 gctggcgtcg cttcaatatg gcgctcttca gccgttaatc cctttatata tttactgtat
    26161 ttccaccacg caaagccaag gaagataaca ataccgccga cgttataaaa aatgatggtc
    26221 agaatattcg cgccagtcgg gaatgtcgaa gcgacaaacc ctacggcaaa tatcgcaatc
    26281 agcattgaaa caacgataat accggtgcgg cgggagccca tacggaaatc gcgtggcagg
    26341 tgatctaatt tggcgcgtaa attcaggtaa gccagcatga tgaataacgg cggaagcatg
    26401 gacgctgcgg cggtcatatt aataatagta ttcatcagat cctgcacggt attggaaccg
    26461 agcatcggga taatcatcag cgggatgacg atcaggaact ggatccacgc tgcgcgcgcc
    26521 ggaacgccgt tttcgttcag ttcgacggtt ttcttaccaa agatgccttc cgggatttcg
    26581 gagaagaaaa ttttcaccgg cgttgcggtc cacatcagca gggaaccgaa catcgcggta
    26641 aaggacacca gaccgacaaa gcgattcatc aacgcttccg gtagaccaaa atacgccgcc
    26701 atgccgtgga atacctgcac cgatccgccg gtaaattcaa ctctttgctg ctgacgaaga
    26761 cgttaatcag caccgaggag acggaataca gtacgccgat aaaaatcccg gcgaggatga
    26821 tcactttaac gaacgatttc gaaccgcctt tgacgtcgtt aacgtacaca gcgacggact
    26881 ccgcgccacc tgcggccata aagatccagg tggtaacgcc gaggaacgcc cagttgaagt
    26941 tcgggatcat cgcgtcaacg gtgatggcgt cagcaggctg tacgccgcca accagcgccg
    27001 tacctgccag taaaatgtag gagagcgtta acagcagcat cagcgttgaa gtgacggagg
    27061 taattggccc caacattttc gccccgttgg tggaaaccca ggtggagaag gcgaacagca
    27121 ccatactgat aatggtggtg gcaaccggcg tcataatata ttcgtatccg aggaaggcat
    27181 acgaagcata agcaataacg cgcggcaaca gtgaggtgaa aaagaacagg tttacgaacc
    27241 agtaggtata ggcagtaata aatgcccaac gtccgcccag cgaacttttt acccacgcgt
    27301 agacaccggc ttctgagttt ttatttaacg aaacaaattc tgcgatgatc agacagaagg
    27361 gaataaaata aaaaatcgtc gcgaggaaaa acatcggtgc cgaggccagt ccaagctcaa
    27421 tattattatt gataacgttg ttaaagctgt aaacggcggc aaaagtcagc gagagcaagc
    27481 cgaatttgcc gattgtatta cgtttggtat cagacataac acatctccga aaatgagtag
    27541 ggtagcggtg gcggcaaact ccttttgccg ccgtagttgt tatttgttat ggaaataacc
    27601 atcttcgatg gtgactttga gaaccacttt tttgaccgcg ttattgcaga taaaccgata
    27661 cgcttcatgg atatcgcaaa taacgatttg cccttcgtgg acctcaacgg tttctccgca
    27721 gccttttaaa tattcacggt cagtttcatc acgataatat tccactacct gtaatgtctc
    27781 tttcggcgca tattcaattt tttgctgccc ttgcaggtag taatgcactt caaaatagcg
    27841 tcgatgcccg gtaaatagcg catcggtcgc ggagtctgtc tccacgcggt aagtcaatga
    27901 gtcaccgatg gagtgggcga cgccaggctg aatgttgtcg atattttcaa tcgcttcaac
    27961 gcagcgttgc cacttcttgc cagaggcgta aatctggcgg aactgttcta agttatcgat
    28021 gatcctcatt gcttattctc gctgtgcaaa ttcgtggaaa agaaccctgc gccgaactca
    28081 tacgacgcca ggctttgcgc ggtagcttct ccgccagaaa ccggcagcaa cgtaaagccg
    28141 tagctgaagt cacggaacca gacgcgccag gagtccagca cctcgctgcc ccaggagttg
    28201 gagccgaggc caagcagctg gtgatcgagg ttcagggtga tgtcatcact gcgctgcagc
    28261 tcgttacagt gctgggcagc gtggatgttt tcctgggtat agtgccaggc gctgaagtta
    28321 attgggcgct gcggaaccac cagcagaccg ttaccgtggc ggttagtcag tgccgtccag
    28381 cggacatgct gacggttacc gttgttctgc gggaagggat agttctcgaa catggcatcg
    28441 acggtgctgc gccagatatc gatgatgtta gcctgctggc tgtcggcgta gttttcgccc
    28501 ggtccacgac cgtaatacgc cacctgatcg tattcgccgt taattcccat ggtgaaaccg
    28561 atgcacggaa tgatgtgcgg atagtcgccg taacgctcgc cggaaagcgc cacgttaacc
    28621 tggccatcgg cagcgatgcg ccagatgtag gtgcagcgca tcccgaagtc aaacaccggc
    28681 ggggcaataa ctgtgcggct gatgatcagc acttcaccat cgctctgttc tacggcaaag
    28741 tcgcgcagat gttcctgcat gatctgcaaa tgattcggtt gccacagccc ttcgtactcc
    28801 tgcttgtggt tgtcgatcat cggcttgaag aagttgatct ttggctcgcg agtcagcagc
    28861 gattcgccat tcacctgcca ggatgtcggt ttgccactca tttttgagaa ggtgatcgcg
    28921 aagttgtagc cgcgaacggt gcagctcaaa cgatcgtctt ccagcgtcag cggacgcgca
    28981 ttatttggtg cgaaaggcac tggctgcgcg gtgttttcct tcagcgggaa ctgataagtg
    29041 gcgatagggt gtccggcttc gctgtagcgg gtgcgggaat ctttggtcac cgtaatgttg
    29101 aggaacgctt cgcgggcgtc cagctgcggc agcgtgatct gcaagggggc ttcgctgttc
    29161 ggcgcaacgt cgcgcagttt aatctgctgc gtcgcgagcg tttcaccttc ggcgcgcacc
    29221 tctgcgtgca gggtgtagtc atcaagcgtg gtaaaccaca gtttattttc gactttcaac
    29281 tcgccgcgag tcagatcccg cgcgtggatt tttaccggcg cgataacctg tttgtactct
    29341 ttcaggcccg gtcccggcgt ctgatcggaa tagatcaaac catcaagaca gaagttatag
    29401 ttgttgggat agtcgccgta gtcgccgccg aatttatacc agacattgcc gtggtcgtcc
    29461 tgtgcctgga tcccgtggtc gcaccactcc cagacataat gaccctgaat gcaatcgtgc
    29521 ttatagaaga cgttctggta ctccgtcagc ccgcccggtc cgttccccat cgcatgagca
    29581 tattcacaga tgatgcgcgg cttcggatgc gggtattcac caaactcatt catcagcggc
    29641 acgcgggtgt acatggtgga aataatatcg accacttcag catcgcgatc ttcttcgtaa
    29701 tgcaccagtc gcgtgtcatc cagcgctttc gccgcatggt acatcgcgcg gatgttacag
    29761 ccatagccgg attcattgcc cagcgaccag atgatgatcg acggatggtt tttctgcgcg
    29821 tggatatggc gaacaatgcg ctcgacgtag actttttccc actgcggatc gtcggtaata
    29881 cggctaatat cgccgacatt agcaaagccg tgcgattcga cgtcggtttc cgccatcaca
    29941 aacaggccgt agatatcaca cagttcgtaa aaacgcggat cgttcgggta gtgagcggta
    30001 cgcacggagt tgatattgtg ctgcttcatc aactggagat ctttctcgac gcgatccatt
    30061 ccaacggcgc ggcctttgcg atgatcgttg tcgtgacggt tgacgccgtg cagcatcaca
    30121 taacggttat tgatccagaa cagaccgtcg cgcactttga tatcacggaa gccaacgcgt
    30181 tgtggcacca cttccagaac gttgccgttg gcgtctttca gcgtcatgac cagatggtaa
    30241 agataagggg attctgctga ccattgctgc ggctgttcga cagtaaaagc aaagctggcg
    30301 ctggtcagtt tttcaattgc caaatgatca atggcgctgc tgtgcaccac gcgttcgcca
    30361 tcaaacaggg tatattccag cgtcgtgacg ataggggagg cggcgagatt ttccagcacc
    30421 acttcgcagg aaagcgtggc atcgcaatag gcttcgtcaa agtcggtacg cacagtgaaa
    30481 tcgttaatat gcgttaggtg ttttccgacc agataaacat cgcggaagat ccccgctgac
    30541 caccacatat cctggtcttc cacgtaggta gagtccgccc actgcatcac gcgcacacac
    30601 aacaggttgt cgccggtttt aaccatcgcg ctgatgtcaa actctgcggt caggcgactg
    30661 cccttgctga aacccacata ctgaccgtta acatagactt caaaataggt ttcgacgccg
    30721 tcaaatttaa tcagcgtctg tttaccctgc cagccgtcgc tgagggtgaa aatacgttga
    30781 taggcaccgg ttgggttatc gctggggaca aacggcacat cgatggggaa cggaaaacct
    30841 tcgtcggtat attgcagttt gccgtgacct tccatttgcc acatggcggg gacggtaata
    30901 tgcccccagt cagccattaa ctcagaggtg aaggcttctg gtacttgcag cggatggtca
    30961 aaaaagtgga aattccactg accgcttaag ggcagaaaca ggctgctggt ttcgcgggca
    31021 aaggtacgcg cttgcgcaac agaatcatat gaaaaaaagt acgcacgcgg cgcaagtcgg
    31081 ttttcgtggg tgagctgaat gttttcccag cgattcatag ggcctcccgg tagagagaaa
    31141 tcggaaccat tcatggtgat ggtagtttgt gtaattgctg gggagtttag taaaattttt
    31201 actaaaaagt agcaggtaaa tgcatttact ttaactggat cacgaaaata aacggcagca
    31261 ctgtccggca aaaacgattg ccggacagtg tgtaagggga tttaacgggt cgtgccgcgc
    31321 agttttaatt tgctgggaac gaagactaac agcggcagcg cgcgaccatc gcgggctttt
    31381 tcatacacca ggttaacgcc ctgacttccc atcatctcgg aatggatgcg cacggtggag
    31441 agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa cgctgataag cgaaatatcc
    31501 tgtgggatgt tcaggcctcg ttcatgaatt gcccgcagta cgccgatagc aatggaatcg
    31561 gaagcaacaa acagtgcctt cggatagtct tcccgcgcca gcatttgttt tgccagttca
    31621 taacccgacg aactggaaaa accgccgcgc cagatatcct cttcgcgtac cacttgtttc
    31681 agtcggccat attccgcaaa ggcgacctca cgaatatccg ccttgccagg ctcatcttca
    31741 ccgccaataa aaccaatacg attaacgccc tggttgatat agaagtcgat gatttcttta
    31801 ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc tgccgggttc gtgaaagtcg
    31861 ataaaacaga tattgtcggt caacgcgctg gcagcggcgc gcagggcggg cgtgggtttg
    31921 ccgacaatta aaataccggt gacgttttta atgtctggta agccgctgtg ttcataacag
    31981 ttggtgagct cgatgcccag cttttcgcac tgggtttcaa tgccgtggcg gatcgccaga
    32041 tagtaaggat cgttgatctc cagctcctgc tggtagctgt agatagccag aatatggtgt
    32101 tggttgactg cacctgtctg gagtttacgg gcactactgg tcttgtactc cagcttttcg
    32161 gcgatctcga gaatgcgatg tttcgtctct tctttcacat tcaatgtcgg atcgtcattt
    32221 aagaccctgg atactgtcgc cagggatacg ccagcttcga ttgcgatgtc ttttagtgtt
    32281 gccatgttta ccttttttac tttccctgat gtgcgccccc attgtagagg aagtgcttat
    32341 cgggtcacct gttttcagta aataaagcgc gtttctttga cgctgtaacg gtaacacagc
    32401 ggctcacgaa tgtgatcacg cctgcattct ccatttttag cctacaggat tgagaaaaac
    32461 ttttgctata gtttagtaaa attttaccct aagggaggaa gcaatggaaa ccgtggctta
    32521 cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt gtggaagtga aacgccatga
    32581 aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg caaaaaacgc tgcaaaccgt
    32641 gaccagcctg gtgccgtact acagcgaaga agagctgatg gggaaaacgg tggtggtctt
    32701 atgcaatctg caaaaagcga aaatgcgtgg tgaaacgtcg gaatgcatgc tgttgtgtgc
    32761 ggaaaccgat gacggcagtg aaagcgtgct gttaacgccg gagcggatga tgcctgcggg
    32821 cgtacgcgtc gtataaaaag atcggatggc gacgtcgtat cgccatccga tttgatatta
    32881 cgcttcttcg acacttactc gcatggccgc cagcgcctta cgcgccgctt tgatcaccag
    32941 ttcacactgt tcaatggtca gtgtcagtgg cggttcaatg cggatcgttt tggcgttatt
    33001 gagcgttccg gccaccagta cgcgctggcg gaacatctcg ctggcaaagt tatagccgat
    33061 ttcgttatca acaaactcaa tcgccatcaa catcccttta ccacgcgctt cctgtaccag
    33121 atcgggatat tcccgcgcca gttgacggaa accgtccagc aacatatcgc ctttttgctc
    33181 agcctgagcc ggtaagttct gctccagcaa cacattgatg gtcgccagcg ccgccgcaca
    33241 ggccagcggg ttgccgccaa aggtggtggt atgcaggaat gggttgtcga acagaactga
    33301 aaacacctct tcagtggcga tggtcgcgcc aatcggcatc acgccgccgc cgagcgcttt
    33361 ggcaaggcag aggatatccg gctgtacgtt ctcatgctcg caggcgaaca tcttgcccgt
    33421 gcgccccatg cccgtttgta cttcatcgag gatcatcagt gcgccgaact catcgcatag
    33481 cttacgtacg gcggtgagat agcccggcgg cggcagaatt acgccacctt caccctgaat
    33541 cggttcgagg atcaccgcag ccacatcatc accggttttt ttgcactcgt taagagccgt
    33601 gcgcatggct tcgatattgc caaacggcac atgacggaag cccggcagta acggcataaa
    33661 cggtttgcgg aaggtcgatt tcgccgtggc cgacagcgcg ccaagtgatt taccgtggaa
    33721 cgcgccgctg gtggcaataa aagtaaactt gccgcgcggt gactggtaag ctttcgccag
    33781 cttcagcgct gcttcaacgg actcggtgcc gctattacag aagaagctgt atttcagttt
    33841 accgggcgtt agcgcagcaa gggttttcgc caacatcgcc cgtaacggat cgagcagctc
    33901 ctggctgtgc agcggttgtt tcgcaagttg attctgtacg gcggaaacca caactggatt
    33961 acggtgcccc acgttgaaaa ttccaaaacc tcccaggcag tcgataaact cctggccctg
    34021 ggtgtcgaca agcgtattta aacttcccgc ttgccactct acggctccgt aatccccgcc
    34081 ggcggtaaca gatttgcgat actctaaaaa ccccggattg acatgctctt tgaagtattc
    34141 aatcacctct cggttaagtg ctttcatctc ctcatgatcc agcgttcgct tctcaatgag
    34201 attcagggcg tgggcgctgc acgctaaagc cgatgcgctc gaaggtaacc tgttcaaaat
    34261 atgctccgga ggctcgcgta tcacatgata ccgatttaag tattgcaggg attgcgccac
    34321 tccggctgcg atcgcgaaaa tcgggataaa cacaaattcg gttatcattt gtgcaatatt
    34381 taatgctatc tgttaacatt tgttgttatt ttgcacggtt tttgtcgggt atggatttga
    34441 tgattgctgc gcaaatgcac gttttcaggg caataattgc ctgatttttg ggcaagataa
    34501 ttaacgtttt cgtatagtaa cagagtgaag gtattttaaa tcaaggaatt agatttgtgt
    34561 ttaaatcgca aattgcgatc taaatcaaat taatcggtta aagataaccg cagcggggcc
    34621 gacataaact ctgacaagaa gttaacaacc atataacctg cacaggacgc gaacatgtct
    34681 tctcatccgt atgtcaccca gcaaaatacc ccgctggcgg acgataccac tctgatgtcc
    34741 actaccgatc tgcaaagcta tatcactcat gctaatgaca cttttgtgca ggtgagcggc
    34801 tataccttgc aagagttaca agggcagccg cacaacatgg tgcgtcaccc ggatatgcca
    34861 aaagcggcgt ttgcggatat gtggttcacc ctgaaaaaag gggagccctg gagcggcatc
    34921 gtgaaaaatc gccgcaaaaa tggtgaccat tattgggtgc gggccaatgc ggtaccgatg
    34981 gtgcgcgagg gaaaaatcag tggctatatg tcgattcgta cccgggcgac ggatgaagag
    35041 atcgcggcgg tggagccgct gtacaaagcg ttgaacgccg gacgtaccag taagcgtatt
    35101 cataaaggcc tggtggtgcg taaaggctgg ctgggtaaac tgccttcatt accgcttcgc
    35161 tggcgggcgc gtggagtgat gaccctgatg tttatcttgc tggcggccat gctttggttt
    35221 gttgctgccc cggtggtgac gtatatcctc tgtgcgttag tggtattgtt ggcaagcgcc
    35281 tgttttgaat ggcagattgt gcgcccgata gaaaatgttg cccatcaggc actgaaggtg
    35341 gcgaccggag aacgtaatag tgttgagcat ctgaatcgca gcgatgagct ggggctgaca
    35401 ttacgtgcgg tagggcaact tggcctgatg tgccgttggc taattaacga tgtctcaagc
    35461 caggtgtcca gtgtcagaaa tggcagtgag acgctggcga aaggcaccga tgaactgaac
    35521 gaacataccc agcagacagt tgataacgtt cagcaaacgg tggcgaccat gaaccaaatg
    35581 gcggagtcgg tgaaacagaa ctctgccacg gcgtcggctg ccgataaact gtcaatcact
    35641 gccagtaatg cggcagtgca gggtggggag gcgatgacca cggtgatcaa gacaatggac
    35701 gatatcgccg acagtaccca gcgcattggc accattactt cgctgattaa cgatattgcg
    35761 tttcagacca atattctggc cctgaatgcg gcggtggaag cggcgcgtgc cggcgaacag
    35821 ggcaaaggtt ttgcagtggt ggcaggggaa gtgcgtcatt tagccagccg cagcgctaat
    35881 gctgccaacg atattcgcaa gctgattgat gccagtgctg ataaggtgca atccggttcg
    35941 cagcaggtac acgccgccgg acggacgatg gaagatattg tggcacaggt gaaaaacgtc
    36001 acccagttga tcgcccagat tagccattca acgctggaac aggccgatgg gctttccagc
    36061 ctgacccgtg cagtggatga gcttaacctg atcacccaga aaaatgccga gctggtggaa
    36121 gagagtgcgc aggtgtcggc gatggtgaaa caccgcgcca gccgactgga agacgcggtg
    36181 acggtactgc attaatcggt atgccggatc cggcggttgg agcacaatgc ctgatgcgat
    36241 gctggcgcat cttatcaggc ctgcggggtg tgtagcgggt gtaggccgga taaggcgcgt
    36301 caagcgtcgc atccggcaat tgcaccgcgc cactggcgga tgcggcgatt aatccagttg
    36361 cgtaatatca aaagcggcgc ggtcgatcac cgcaatgatc ttttttattt gtgcatcact
    36421 gatatcgctc tggttgacgc gtaaatccag caccgcttta aaattatcca gcgcccgctt
    36481 catttgcggg ttctggcgca gcgccgcgcc aacgcaacgc gctttgatgc gttcttcaat
    36541 catctccacc tgttcgcggt tttcttccag ccactgcgcg ccttgttcgg tcagcgcaat
    36601 ctgcttctta cctccttcct cttcgcggat ggtaatcagc gactgctcct gcagaaaatc
    36661 cagcgttggg tagatgacgc ccgggcttgg ggtgtaattc ccctgggtta gattctcaat
    36721 cgctttaatc aattcgtaac cgtggctgtc atcgcgcgag agaatatcca gaatcaccag
    36781 acgtaattca ccgtgaccaa agaaacgttg ccgacgaccg ccgccgcgac cgtggcgacc
    36841 accgcagcat tgaccgtgtt catgctggtg accgtgtccg cagtgctcgt gttctgactt
    36901 ctcacctttg cagcagccct catggcgtgg ctggccttca tgtttacaac acccttcgtg
    36961 atgatggctc atatcagcct ccttttgctt aattagatat atctaattta tatctgatat
    37021 gagcgttctt gcaagtgtaa aaatgatttt taaacgtgga ctcatctcac acgtaggccg
    37081 gataaggcgt tcacgccgca tccggcgttg agtaatgatg cctgagaaga ggcggttcgt
    37141 cttatcagac tatagacata tcagttctac aaatcgcttg catttatcat gattaacaat
    37201 cattatcatt tgcgagtttt atttagatat atctgattac catcacgaag gcgataacaa
    37261 tgaataacac cccccgctac ccgcagcgcg ttcgcaatga tctgcgcttc cgtgaactga
    37321 ctgtgttacg cgttgagcgc atcagcgccg gttttcagcg cattgtcctc ggcggcgagg
    37381 cgctggacgg ttttacatcg cgtggctttg acgatcacag caaactcttc tttcctcaac
    37441 ctgacgctca ctttgtgccg ccaacggtaa cggaagaggg catcgtctgg ccggaaggac
    37501 cacgcccacc gtcgcgtgac tatacgccgc tgtatgacga actacgccat gaactggcga
    37561 ttgatttctt tattcacgac ggtggggtcg ccagcggctg ggcgatgcag gcgcaaccgg
    37621 gcgataaact tacggtggca ggtccgcgcg gttcgctggt ggtgccggaa gattacgcgt
    37681 atcagctgta tgtctgcgat gaatccggaa tgcccgcatt gcgccgccgc ctggaaacgt
    37741 tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt tagcgtgcgg gataacgcct
    37801 gtcaggatta tctcgcgcac cttgatggtt ttaatatcga atggctggca catgatgagc
    37861 aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc cgatgattac ttcatctgga
    37921 taaccggcga aggtaaagtc gttaagaatt taagccgccg ctttgaagcg gaacagtatg
    37981 acccacagcg ggtacgtgca gcggcttact ggcacgcaaa gtaactgata tataaaaaga
    38041 aaaaggctga cgatttctcg tcagcctttg ctatatctgg tggcccctgc tggacttgaa
    38101 ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctaaggg gccatggtag
    38161 cggattataa agtaactccg tgtcgcaatc cagccattac cgcacgcctg ctgtttttat
    38221 aaacaatgta ttttcaatcc gttatacttt tcctggtgat gttagaaagg agtaaatatg
    38281 gttgaggata ttttggctcc agggttacgg gtcgtgtttt gcggtatcaa ccctgggctt
    38341 tcatccgccg ggactggttt tccctttgct catccggcaa atcgcttctg gaaggtgata
    38401 tatcaggccg ggtttaccga ccgtcagttg aagccgcagg aggcacagca tctgctggat
    38461 tatcgttgtg gcgtcaccaa actggtagac cgtccaacgg tgcaagccaa tgaagtttca
    38521 aagcaggagc tacacgcagg cgggcgtaag ctgattgaaa aaattgaaga ttatcagccg
    38581 caggcgttgg cgattctggg caaacaagca tatgaacaag gattcagcca gcgcggtgca
    38641 cagtggggga aacaaacgct caccattggt tcgacgcaga tttgggtgct gccaaatccc
    38701 agcggtttaa gtcgcgtttc actggagaaa ctggttgaag cgtatcgcga gctggaccag
    38761 gcgctggtag tgcgtgggcg ataaaaaacg ccaccgaaaa ggtggcgttt gtgcagaggg
    38821 cctagtgccg ggtgcggcgt aacgcctgat ccggcctacc gattaatcgt ccaggaagct
    38881 acgcagcact tcagaacggc tcgggtgacg cagtttgcgc agcgccttcg cttcgatctg
    38941 acggatacgt tcgcgggtaa cgtcgaactg tttacccact tcttccagcg tgtggtcggt
    39001 gttcatatcg ataccgaaac gcatacgcag aacttttgct tcacgcgcgg tcaggccagc
    39061 cagcacgtcg tgcgttgccg cacgcaggct ttcggtggtc gcagaatcca gcggcagctc
    39121 gagggtggta tcctcgatga aatcccccag atgcgaatct tcatcatcac cgatcggcgt
    39181 ttccatggag attggctctt tggcgatctt cagcactttg cggatcttgt cttccggcat
    39241 cagcatacgt tcagccagtt cttccggcgt cggttcacgg cccatctctt gcagcatctg
    39301 gcgagaaata cggttgagct tgttgatggt ctcaatcata tgcaccggaa tacggatggt
    39361 gcgcgcctga tccgcgatag agcgggtgat cgcctgacgg atccaccagg ttgcgtaggt
    39421 ggagaacttg taaccacggc ggtattcgaa tttatcaacc gctttcatca gaccgatgtt
    39481 gccttcctga atcaggtcaa ggaactgcaa gccacggttg gtgtatttct tagcgataga
    39541 aataaccaga cgtaagttcg cttcaaccat ctctttcttc gcacggcggg ctttcgcttc
    39601 accgatggac atacgacggt tgatatcttt aacctgctcg atggtcaggc cggtttcttc
    39661 ttcaatctgc tgcagttttt gcagggcgcg atgcacttct tcagagacat cgtgcagttt
    39721 ttccgaccac ggcttgttca tcgcaattgc cgcgttgaac caggtatcgc tggtttcgtt
    39781 gccggtaaac agggtaatga agtttttctt cggcattttg cactgctcaa cgcagagctt
    39841 catgatcaga cgttcttgcg tacgaacgcg gtccatcatg acgcgcatgc tgttgaccag
    39901 gtagtcaaac tgcttcggca ccaggcggaa ctgtttgaat acttcagaca gtttcaggat
    39961 ctcttcctga gcggtagcgt gactgcgacc tttcgctttg atggtgtcac gcgttacaac
    40021 gtactgagcg cgtagttccg caaatttttc gcgagccagt tccgggtcga tgctgttgtc
    40081 atcatcggcg ctgtcgtcat cgccatcttc ttcgtcttca tcttcgtcat cgtccagatc
    40141 ttcctgggaa agctcagaac cgacgtgagt ggcggtaggt gccagatctt cttctgcgtt
    40201 cgggtcaaca aagccggtga tcagatcgga cagacgcgct tcttctgctt caacacgatc
    40261 gtactgttcc agcagatagg tgatcgcttc cggatattca gcaacggagc attgaacctg
    40321 gttgatcccg tcttcaatac gcttagcgat gtcaatttcg ccttcgcggg tcaacagttc
    40381 aacggtgccc atttcacgca tgtacatgcg taccgggtca gtcgtgcgcc cgatttcaga
    40441 ttccacgctg gaaagcacct gcgcggcggc ttcggcagca tcttcgtccg cggtgttttc
    40501 agccagcatc agatcatcgg catccggtgc ttcttccatc acctgaatgc ccatgtcgtt
    40561 gatcatttgg atgatgtctt cgatctgatc tgaatcgacg atatcttccg gcagatggtc
    40621 attgacctcg gcataggtca gatagccttg ctccttacca cgggtgacaa gaagtttcag
    40681 ctgtgactgc gggttttgct ccataagacg gtatccacac ttatttcatg aggttggtgt
    40741 tgggcgattg acccgatgtc cttcagccgt taacgctgaa atccaacggt tgtttaccgc
    40801 caacgataat tacgagggcg tacttatata tttgccgctg cctctcagtg cggctgtcgg
    40861 gggcttcccg atcgctcttc ggcacttaag ccgttaaatc actttttcgc cagctcctgg
    40921 tttaatgtcc agagctccag gcgttcttcg ttgcttaaac catgcgtgcg ctcacgagcg
    40981 attaactctt cctggcgcag ttcaagcagc gaatcaaaca tatggttgag tgagtcggtg
    41041 aaggtttgct cagcaatatt cttatctgct atatcgtccc acatcgacag tttttcaagg
    41101 gtggcagcat tatttgtacc acgatagtgc tctaaaagtt gcccggtggt cagacctggc
    41161 tgggagagac aagtgttgac cagttctctg aataagccaa gtccagggag cttattttca
    41221 tccagattct caagcggcgg gaccaacgtc gctaattctg gattttgcac cagcaaccct
    41281 ataagtatac gcatggtcgt gcgttttagc tgcggaacag ggcgagaaac gccgctctct
    41341 gccgcttttg gcattaatcg ttcaagctgg ctgtcatcaa gtatgcctaa tttgttgcct
    41401 aattcctgac gaagatatat tcgcagcgtt tcgcccggca cttgcgatat caatggtagt
    41461 gccagcgtac tcaaacgtgc gcgcccgtca ggggtactca gatcaacttg cggcatcaga
    41521 ctgttaaaca gaaatgcgga gagtggcatc gcctgctcca tccgcgcttc aaacgcttct
    41581 ttaccttctt ttcgtactag cgtgtcaggg tcttcgccat caggcaaaaa cataaagcgt
    41641 agctgacggc cgtctgtcat gtaaggcagc gccgtttcca gcgctcgcca ggcggcatcg
    41701 cggcctgcac ggtcgccgtc ataacagcaa atgacattgt tggtcgcgcg gaacaacagt
    41761 tgtatgtgat cggcggtggt tgacgtacct aacgacgcaa cggcgtaatt aatgccgtat
    41821 tgcgccagcg ccaccacgtc catatagcct tcgaccacaa gcagacgatt gggttcagcg
    41881 ttatcctgct gcgcttcata aagaccgtaa agctggcggc ctttatggaa aatgtctgtt
    41941 tccggcgagt tcaggtattt gggggtatcg ttgcccagca cgcgcccgcc aaaaccaatc
    42001 acccgaccgc gtttatcgcg aatggggaac atcacccgct cgcggaaacg atcgtaactg
    42061 cgtccctgat cgttagtgac caacatcccc gcatcaatca atgactggcg attttctgga
    42121 ttgccgccaa accgcttcag gacgttgtcc cagccggggg gcgcaaaacc aatcgcaaag
    42181 cgagcgataa cctcgtggct taatccgcgt ttttccagat actggcgcgc agacgtggca
    42241 acaggttgtt gtaaagattg ttggtaaaac gtattcagac cgtccatcaa ctgataaagc
    42301 gtttgcctct gatggcgctc gatctggctg gggccgctgc ctgcttcaaa tggcacttca
    42361 agattgtgca ttgctgccag ctcttcgacc gtttcgacga actcgagctt gtcgtagttc
    42421 atcaggaagt cgatcgcgtt gccgtgcgcg ccacatccaa agcagtggta aaactgtttc
    42481 tcaccgttaa cggtgaagga cggggttttc tcgttgtgga atggacaaca cgcgtggaaa
    42541 ttcttgccct gctttttcag cttcacacgg gcatcgatca gatcgacgat gtcagtgcgt
    42601 gccagcagat cattaatgaa tacgcgtggg attcgtccag ccataggccc cgatttttag
    42661 caattcataa acgaaaataa gccgcgcatt cctttcggaa gcacggcctt acaactacaa
    42721 ctcggtctga tcggagagca acgctctcgg ggaattagta cagacgagtg cggcgtgcgt
    42781 tttcgcgagc cagtttcttc gcgtgacgtt tcactgcaga agctttagcg cgcttacgtt
    42841 cggtagtcgg tttttcatag aactcacgac gacgaacttc cgccagaaca cctgcttttt
    42901 cgcaggaacg cttgaagcga cgcagagcta cgtcgaacgg ctcgttttca cgtactttaa
    42961 ttaccggcat gtgcctctca cctttgatta attcggtttg ccgctggcat caacgccagc
    43021 ttatttcaaa atggtgcgga attttactgc aattgctgct gctttgtaaa gcaccgcggc
    43081 cttttttgaa agggactttt ataagggtga ggagtataca cgaggctttc tccaggggcg
    43141 aacaaagttt tacatcaacc cgcattggtc ctacactgcg cggtaataaa gcgaggtaaa
    43201 acaagtcatg cgtgtactgg gtattgaaac ttcctgcgat gaaaccggca tcgccattta
    43261 cgacgatgaa aaaggtttgt tagccaacca attgtatagt caggtgaaat tgcacgctga
    43321 ctacggcggc gtcgtgcctg aactggcctc ccgcgatcat gtgcgtaaaa ccgtaccgtt
    43381 gatccaggcg gcgctaaagg agtctggttt aacggcaaaa gacattgatg ctgtggccta
    43441 taccgcaggc cctggattag tcggcgcgct actggttggc gcgaccgtgg ggcgttctct
    43501 ggcgtttgcc tgggacgttc cggcgatccc tgtacaccat atggaagggc atctgttagc
    43561 gccgatgctg gaagataacc cgccggaatt tccgtttgtt gcgctgcttg tttccggcgg
    43621 tcatacgcag ttaatcagcg tgactggcat tggtcagtac gagctgctcg gcgagtctat
    43681 cgatgatgcc gccggggaag cgtttgataa aaccgcgaag ctgctggggc tggattatcc
    43741 tggcgggccg ttactgtcga aaatggcggc tcagggtact gccgggcgct ttgtcttccc
    43801 gcgtccgatg accgaccgtc cggggctgga tttcagcttc tccggcctga aaaccttcgc
    43861 ggcaaatacc attcgtgaca acggcaccga cgaccagacg cgtgctgata tcgcccgcgc
    43921 ctttgaagat gcggtggtcg atacgctgat gattaagtgc aagcgggcgc tggatcagac
    43981 gggctttaag cgactggtca tggcgggcgg cgtgagtgct aaccgtacgt tacgggcgaa
    44041 gctggctgaa atgatgaaaa aacgccgcgg cgaagtgttc tacgcgcgtc cggaattttg
    44101 tactgataac ggcgcgatga tcgcctatgc cggaatggtg cggtttaaag caggcgcgac
    44161 ggcggatctc ggcgttagcg tgcgtccgcg ctggccgctg gcggagttac cggctgcgta
    44221 aaactattgg gtgccggaga gcaatttccg gcaccgtcct cacttaaagc aacaccacgg
    44281 gcatccacag taagccggta atcaccagca atacgaggaa tatcagccca aaaatcgccc
    44341 ccagtcgcca gtaatccgcc gttggcagat aaccactgcc gtagtaaatc ggactggggc
    44401 cggtggcgta tggtgtaaga atgctcccca gaccaattgc cgcaccaacc atcaggcaga
    44461 acaccggcag cgggatttcc ggcatcgcca gcgcagcggc aatcatcatc ggtgcgagag
    44521 cggaggtata cgccgtggcg ctggcgaaaa agtagcgcag tagataaaac accacaatca
    44581 acgccaccat caccatcgtt ggcgaataac cgcttaagct gcctgctaac agtttgccaa
    44641 accagctaat aaaaccggtg ttgttgagtc cggtggcgag ggtgataagc gaggccagcc
    44701 agaagaaaac gttccacgcc gctttattac tgacaatgtc gtcccaactg ataatgcgca
    44761 gaagcagcat cagtgccacc acgctgtaac cgaccatcgc ggcatcgata taatcaccgc
    44821 cgaaaatcca cagcaccagc gcgcctacca tcagccccag catccgtttt tcacgcgaac
    44881 agagcgggcc cattgcctgc agttccgtct ctgcccagcg cggcacctga tcgctagact
    44941 tcagtaccgg cgggtacagc acgtaagcca gccagggaac cagcagaacc agtaaaatgc
    45001 tgagcggcaa catcccgagg aaccagtcgc cccaactcag cgtggcgtga gatgcgcttt
    45061 tcatcagtcc aattaacagc aagtttggtg ccatcgccgt caagaaaatg gcgctggtca
    45121 cgcagtcggc aacaatcccc atccacatga tgtacgagcc aatgctgcgc gaactgctgt
    45181 cgtttggttg tgattgatag agcggtggca ggttacggat gatgggatag ataatccccg
    45241 caccacgcgc cgagttggac ggtgttacag gtgcgaggat cagctcggag aacatcaccg
    45301 catagccgag aaacagcgtg cgatgtccca tctttttcac cagaatcagc gcaatgcggc
    45361 gtccaagccc ggttttttca tagcctgtgc caaacataaa ggcggcgaaa atcagccaga
    45421 taaccgaatt agaaaaaccg gaaacggccc acgagaggga ttttgcagtg aatttaaagc
    45481 ctggctgagc gagctgctcc gggctgaaca gcagccaggg agagagtatg gcgatgatgg
    45541 atatacccac catcgccacc acggcacccg gcacgggttc gaggatcagt ccaacgatca
    45601 cgccagtaaa aacggcaaag tagagccagg tatggttctc cagccctgcg ggaaccggaa
    45661 gtagagcaat aatggcgatg accgccagcg gcgcaagata tcgccaccat tcagtggaag
    45721 gtttcatcgt gttatgcctg cgaaaaagga gaggggtgag cctctccggg agggttattt
    45781 gatgtaatgg acgtgctcgc agatctcttc cacgatggga tcgcggcgtt cggcgaacag
    45841 ctttttgttt tcggctatca ggttgttgcc gtgggtatca atagagacaa tcagcgggcc
    45901 gaactctttg acccggcaga cccacagtga ctccggcatt ccgagctctg tccagtgcac
    45961 ttcttcaatc tcttccacct gggttgccgc cagcaccgcg cagcctgccg ggaaaatcac
    46021 atgtagcgcc ttgaatttct ggcagccttc ttcggtcagc ggccccatac cacctttgcc
    46081 aaccaccagt ttcacgccgg tctgctcaat aaattcacgt tcaaaacttt ccatacgcat
    46141 actggttgtc gggccgacgg agaccatctc ccatttgtcg ccgtttttgc gcacgatggg
    46201 gccagcgtgg aaaatcgctt tgccgttgag atcgtaaggg attggacgtt tcagttcgat
    46261 caaacggcgg tgacaaacgt cgcggcaggt caccagcgta ccggtcaggt agatcacatc
    46321 gccgacgcga atatcttgca gatcttcagc tttgatcggg gttgtcagga tctttttcat
    46381 aacgcgctcc gggtgtgaga cagattttca aaggtgagat cggcatgaac cagcagcgtg
    46441 ccgcgacgat gcgcccagca gccggtagag acagcaacgc cgatggttga cggatggcgg
    46501 gcggcagatt cgatatgtac gcccatcact gaactgttgc cggtcagccc ttgtggacca
    46561 atccccagac ggttgagtcc ttcttccagg cgtagctcca gttctgccgc ttttggattg
    46621 ggatggcggc tgccaatcgg gcgcaaaatg gctttacgcg agagtacggc ggcggtttcc
    46681 accgaggtgg cgatgcccac gcccaccagt accggtggac aggcgtttac ggcgagggtg
    46741 gagatatttt cgaagacgaa tttcaccacg ccttcgtagc cttctgacgg cattaacact
    46801 ttcgagcggc caggtagcgt gcagccgccg cctgccatgt aaacttcgat ttccgcatcg
    46861 tcattgtcgg ggatgatgtc ccaggtgacc cacggtacgc cgctaccggt atttttgccg
    46921 gtgtttactt cgtcaaaaat ttctaccgca ttgtgacgta gtggcgcttt gacggttgcc
    46981 tcttccacgg cttgtttgag tatgctttgc agctcgccaa gcagtgggaa gcgggaaccg
    47041 actttaacga agaacataat ctccccggtg tcctgacagg caggacgatt caggtcaatc
    47101 gctttttgca tgttgtcgaa catcgtatgg tagataattt tccccatcga cgacgtttcg
    47161 gcatccttta gctgttttag tttatccacc acgtcatcag gcattcgggt agaaatcatg
    47221 gcggtaaagt tagcgacaat ctctgtcaac ttattcactg cctgttgctt attactttcg
    47281 ctcatcatgg caactccagt ttttgtattt gtctggaaaa attcaccgcc acaaactacc
    47341 actaaaaatg tggttttata ttttttgcca ccgtgaatat gtaattaaca gttcgtgaat
    47401 caacgcgaaa ataagaatga atattgcgcc agagcaggga atggcaatgt tttgcgaaat
    47461 acactgcgct tatcacaact gcggataatt aacgatgctg aatagctggc ctttagccaa
    47521 agacttgcag gtactggtag aaatcgttca cagcgggagt tttagcgccg cagcggcgac
    47581 gcttgggcag acgcctgcat ttgtcaccaa gcggatccaa atccttgaaa atacgctggc
    47641 aacaacgctg ctgaaccgct ccgctcgcgg cgtggcgctg actgaaagtg gccaacgttg
    47701 ttacgaacat gcgttggaaa tcctcaccca atatcagcga ctggtcgatg acgtcacgca
    47761 aatcaaaacg cgcccggagg ggatgattcg tattggttgc agttttggtt ttgggcgcag
    47821 ccatattgcg ccagctatta ccgaactgat gcgcaattat cctgagttac aggtgcattt
    47881 tgaattgttc gatcggcaaa ttgatttagt gcaggataat attgatctgg atattcgtat
    47941 taatgacgaa attcctgatt attatattgc gcatcttttg acaaaaaata aaagaatatt
    48001 atgcgcagca cctgagtatc tgcaaaaata tccgcagcca caatccctac aggaattaag
    48061 tcgtcatgac tgtctggtga ccaaagagcg cgatatgacc catggaatat gggagttggg
    48121 gaacggtcag gagaaaaaat cggtgaaggt aagcgggcat ctttcctcca atagcggcga
    48181 gattgtgctg caatgggcgc tggagggcaa agggattatg ctgcgttccg agtgggatgt
    48241 gttgccgttt ctggagagtg gcaaactggt gcaagtattg ccagagtatg cacaaagcgc
    48301 caatatctgg gctgtttacc gggagccgct ctatcgcagc atgaaattac gtgtctgcgt
    48361 ggaatttctg gcggcatggt gccagcaacg gctgggcaag cccgatgaag gctatcaggt
    48421 catgtagatc caccagaaat cactcgggat ccttttcgcg ctttcttttg aatttcgtcc
    48481 agatttttgt ctcctgacga cgccacagac gttggatgtt gtcatgatga cgcagcagga
    48541 tcaggcaaga gagcatcgaa accgggaagg tgaattgtgg cttaaaccac cagacataaa
    48601 acggagcaat cagtgcactg acaatcgctc ccagcgacga gtatccgctc aatagcacgg
    48661 tcagtaacca ggttcccgcc attactccgg tgagatccca gccaatgggt gcgatggcac
    48721 caaaagcggt agcaacgcct tttcctcctt taaatccgaa gaaaacgggc cagatgtgtc
    48781 caagacaggc ggcgatggca attaagccta gccagaaggg gctgacacct aattcatacg
    48841 cgccccagac gggcaacatt cctttcagaa cgtcgaaaat cagtactgct acggctgctc
    48901 ccttgccacc gatacgtaac acattggttg cgcctggatt gccggagccg ctggttcgcg
    48961 gatcgggcag cccacacaag cggcaaacca gaatggcact ggaaatggag ccgcagaggt
    49021 acgcgatgag gatcattcca ggcgcgattg cactcataag ctgttccgtt ttgaaaattc
    49081 gtgttcaacg atgaatctgt ggataatacg cacatttcgc cggaagtggt atccggttag
    49141 ccaaaaagca ggcaggacgt gatggatatt gtatttatag agcaactttc ggtaatcacc
    49201 actattggtg tttacgactg ggaacagacc atcgaacaga agttagtgtt cgatatcgaa
    49261 atggcgtggg ataaccgtaa agcggcgaaa agtgatgatg tggcggattg cctcagttac
    49321 gctgacattg cagaaacggt ggtcagccac gtcgaggggg cgcgttttgc gctggtggaa
    49381 cgcgtggctg aagaggtggc ggagctgctg ttagcacgct tcaactcgcc gtgggtgcgt
    49441 atcaaactca gcaagccagg cgcagtggcg cgggcggcga atgttggcgt aatcattgag
    49501 cgtggcaata atctgaaaga aaataattaa ttttacagct gttaaaccaa acggttataa
    49561 cctggtcata cgcagtagtt cggacaagcg gtacatttta ataatttagg ggtttattga
    49621 tgagcgatat gcactcgctg ctgatagcgg caatattggg tgtggtcgaa ggattgacag
    49681 aatttctgcc ggtatccagc acgggccata tgattattgt cggtcacttg ttggggtttg
    49741 agggcgacac ggcgaaaacc tttgaagttg tgatccagtt aggatcaatt ctggcggtag
    49801 tagtgatgtt ctggcggcgt ctgtttggcc tgattggcat ccactttggc cgcccgttgc
    49861 agcacgaagg tgaaagcaaa ggtcgtttaa cgctgatcca cattttgctg gggatgattc
    49921 cggcggtggt attggggctg ttgttccacg acacgattaa gtcattgttt aacccgataa
    49981 atgtgatgta tgcgctggtc gttggcggtt tgttgctgat tgccgccgaa tgcctgaagc
    50041 cgaaagagcc gcgtgcgccg ggtcttgatg atatgaccta tcgtcaggca tttatgattg
    50101 gctgtttcca gtgtctggcg ctgtggccgg gtttctcccg ttccggggcg accatttcag
    50161 gtgggatgct gatgggggtg agccgttacg ctgcttccga gttttcgttc ctgctggcgg
    50221 tgccgatgat gatgggcgca acggcgctcg atctctacaa aagctggggc ttcctgacaa
    50281 gcggcgatat cccgatgttt gccgttgggt ttatcaccgc ttttgtggtg gcgctgatag
    50341 cgattaaaac cttcctgcaa ttgattaagc gcatttcgtt tatcccgttc gccatttatc
    50401 gctttattgt ggcggctgcg gtgtatgtcg tgttctttta attgaggaat agtgtcggat
    50461 gcttggcgtt gtcaacgatt gcgtaggccg aataaggcgt tcacgccgca tccggcaatc
    50521 aacgcctgat gcgacgcttg cgcgtcttat caggcctaca actgtcgttg acatgtagcc
    50581 ggataaggcg tttacgccgc atccggcaaa atgcccaata ctcattcagg ctttgggcaa
    50641 cgttgttcct tccagctggc taccgccgca atccgccgtc gggtcagctc ctcgcgaatc
    50701 tccacacctt taaatcccgc ttcaacgacg gcttttgtcg gcactgactg cgccacttcc
    50761 caggcttcgc gcaaccagcg gccttgcggg tagtccgctg attcaaaacc ggttctgccg
    50821 cgcacgtcag cctcgctggt cagcgccagt tgctcgacac gctgcggttt acgccaggca
    50881 tcgatggaat caaataattt gacgatggtt tttgggttca gcattgggaa ggtgtggatg
    50941 agatcgtgaa actcagccac cagtctggct aaatcgcgaa tttcatttgg cacgcgcaga
    51001 cgctggcata gttgttccac taacttaaca cccgccgggc catgaccatg atgacgcggc
    51061 cagagttctg gcggcgtcag ccctttaccg agatcgtggc ataaagttgc gaaacggaca
    51121 tcgacctgcg gactcagcat cgccgccatt gagagcgtca ttaaggtatg aatacccgta
    51181 tcgatttccg gatgccactt ggcaggggcc ggaacgccaa acagtgcgtc aatttccggg
    51241 aataaaacgc gcagtgcgcc gcaatcgcgc agtacctgga agaacacctg tggattgcgg
    51301 gtggtgaggg cgctttccgt ctctttccat acccgttcag gcgtcaggtg ttccagttca
    51361 cccgcatggg tcatctcgcg catcaacgcc agagtttcat cggcaatacg aaaaccgagg
    51421 tgggcataac gcgcagcaaa acgcgccacg cgcaatacgc gtaacggatc ttcgccaaaa
    51481 gcgggggaaa catggcgcaa cagacgattt tgcagatcgc ccagaccgtt gtacgggtcg
    51541 ataatctcgc cgttatcgtc ctgggccagc gcattaatgg tcagatcgcg acgcttaaga
    51601 tcatcttcca gcgtgacatc cggtgcggca tagcaagtaa aaccggtgta accggaaccg
    51661 gatttccgtt cggtacgtgc cagcgcatac tcttcatgcg tttgcggatg cagaaacaca
    51721 ggaaaatcgc ggcctacctg ctggtagccc gcgtcgagca tctcctgtgg cgtactgccg
    51781 accaccaccc aatctctgtc tttgaccggt agccctaaca atgcatcccg aacagcacca
    51841 ccgaccagat aaatcttcac gccatcatcc cttcgtcaaa tacacaattc ctaataataa
    51901 gacagtgcaa cgaagaaggc gatttagttc atccagcgat ctttgcgttt gcggcttggg
    51961 atcaggtgcg gcagtaccag accgagcagc aagccaagcc ccagcacgcc accgccatac
    52021 ataaaccatt gcatgatgat ggtgcgctgt ttgtcatcca gctgtacgct ggcggcatcg
    52081 acctttttct gcgcgacaat cagctcgttt ttcagtttct gattttcttc ttttaacccg
    52141 ttgatcacgc tgtcgctctg cgccactttt tgctgcattt ctgcggtgcg ctgattccag
    52201 gtgttatcga tattggtgag tttatcggtc agggttttga cctgattttc cagatctggc
    52261 acacgggagc gcaggcttgg ctcagtgcta agttgtttca acgggatcca ggcggtacgg
    52321 ccagagctgt ctttcacctg ggcataattg gtgttggcgt cagtttgtaa taaggtcact
    52381 tcctcgccgg cgttaaccgt gcccacgagg cgataatgat ctcccggacc gctgcggacc
    52441 caggtattca gttcgtcgga aacatagcgc gtttcttcag cgtgtgagac ggcagtcgcg
    52501 ctaagtgcga gtaaagttaa tccgatcagg cgtaattttg gcatcaggct gtcgttattg
    52561 tcatggaaag tggaacgata gtagtggcat cagtgtcgct acgcaaagca ttcgacatca
    52621 atcggaatcc tctgtgtcag gtcttgccac tgcttcaaac ttttacgtcc gtcaaattgc
    52681 ccgttgcatg gcaatttggc gcaaaatact atctactgac aaaaaagatc gcattaagtt
    52741 cgccgttcat cgtcacttat gtgacataac cgataagtaa aggccatggc tcaggaaatc
    52801 gaattaaagt ttattgttaa tcacagtgcc gttgaggcgt tgcgtgacca tctcaatacg
    52861 ctgggcggcg agcaccatga ccccgtgcag ttgctgaata tttactacga aacgccggat
    52921 aactggctgc gtgggcacga tatgggctta cgtattcgtg gcgaaaacgg tcgctatgag
    52981 atgaccatga aagttgcagg aagagtgaca ggcggcttac atcagcgccc ggaatataac
    53041 gtggcgttga gcgaaccgac gctcgacctg gcgcagttac cgacggaagt ctggccgaac
    53101 ggcgaattgc ccgccgatct cgcctcccgc gtgcagccgc tgttcagcac cgatttttat
    53161 cgcgaaaaat ggctggtggc ggtcgatggt agccaaattg aaatcgccct cgaccagggg
    53221 gaagtgaaag cgggtgaatt tgctgaacct atctgtgagc tggaactgga actgcttagc
    53281 ggcgacacgc gcgcggtgct gaaactggcg aaccaactgg tatcgcaaac cggattacgc
    53341 cagggcagcc tgagcaaagc ggcgcgtggc tatcatctgg cgcagggcaa tccggcgcgt
    53401 gaaatcaaac cgaccaccat tttgcatgtt gcggcaaaag ccgatgtgga acaggggctg
    53461 gaagcggcgc tcgagctggc gttagcgcaa tggcagtatc atgaagaact gtgggtacgc
    53521 ggcaacgatg cggcgaaaga acaggtgctg gcagccatta gcctggtccg tcatacgctg
    53581 atgctgttcg gtggtattgt gccgcgtaaa gcgagcactc acttacgtga tctgctgact
    53641 caatgcgagg cgaccattgc ttctgcggtg tctgccgtga cggcggtcta ctctaccgaa
    53701 acggcaatgg cgaagctggc gttgaccgaa tggttggtaa gcaaagcatg gcagccattt
    53761 ttagatgcca aagcgcaggg caaaatcagc gactccttca aacgctttgc cgatatccat
    53821 ctttcccgcc atgccgctga actgaaaagc gttttctgcc agccgttagg cgatcgctac
    53881 cgtgaccagt tgccacgcct gacgcgtgat attgactcaa tactgttgct ggcgggttac
    53941 tatgatcctg tcgtcgcgca agcctggctg gagaactggc aggggctgca tcacgctatt
    54001 gcgaccgggc aacgcatcga aattgaacat ttccgtaatg aggcaaacaa tcaggaaccg
    54061 ttctggttgc acagcggaaa acgttaatca ggcaaggata aaatcgctta tgaagccgct
    54121 ctcttcaccg ttacagcagt actggcagac cgttgttgag cggctgccag agcctttagc
    54181 cgaggaatca cttagcgcac aggcgaagtc agtacttact tttagtgatt ttgtgcagga
    54241 cagcgtgatt gcgcatccag agtggctgac ggaactggaa agccaaccgc cgcaggccga
    54301 cgaatggcag cattacgcgg catggttgca ggaggcgctc tgtaatgtga gtgacgaagc
    54361 cgggttaatg cgcgagctgc ggctattccg gcggcgcatt atggtgcgca tcgcctgggc
    54421 gcaaacgctg gcactggtta ctgaagagag catattgcag cagctcagct atctggcgga
    54481 gacgctgatt gttgcggcgc gtgactggct gtatgacgcc tgctgccgcg agtggggaac
    54541 gccgtgcaat gcgcagggcg aagcgcaacc gctgctgatt ttaggcatgg gtaagctggg
    54601 cggtggggag ctgaatttct cctctgatat cgatctgatt tttgcctggc cggaacatgg
    54661 ttgtacgcag ggtggacgcc gggaactgga taacgcgcag ttttttaccc gcatggggca
    54721 gcggctgatt aaagtgctgg atcaaccaac gcaggatggc ttcgtctatc gcgtggatat
    54781 gcggctgcgt ccgtttggcg aaagtggccc gctggtgctg agctttgccg cgttggaaga
    54841 ttattaccag gagcaggggc gcgactggga gcgttacgcg atggtcaagg cgcggattat
    54901 gggcgatagc gaaggcgtct atgctaacga gttgcgtgcg atgctgcgcc cgtttgtttt
    54961 ccgtcgttac atcgatttca gcgtgattca gtcgctgcgc aacatgaaag ggatgattgc
    55021 ccgtgaagtg cgtcgacgtg gtttgaccga caatatcaaa ctcggcgcag gcggcattcg
    55081 cgaaattgaa tttatcgttc aggtgttcca gctcattcgc ggcggacgcg aaccgtcgct
    55141 gcaatcgcgc tctttactgc caacgctcag cgccattgcc gagctgcatc tgctttctga
    55201 aaacgatgct gaacaattgc gagtggcgta tctgttcctg cggcgtctgg aaaacctgct
    55261 gcaaagcatt aacgacgaac aaacccagac gcttccttct gatgagctta atcgtgcgcg
    55321 gctggcgtgg gcgatggact ttgctgactg gccgcaactg accggggcgc tgaccgcaca
    55381 tatgaccaat gtgcgccggg tgtttaatga attgattggc gacgatgaaa gtgaaactca
    55441 ggaagagtcg ctgtcggaac agtggcgtga gctgtggcag gatgcgttgc aggaagatga
    55501 cactacgcca gtgctggcgc atcttagcga ggatgatcgc aaacaggtgc taacgctgat
    55561 tgccgatttc cgcaaagagc tggataagcg caccatcggg ccgcgaggac gtcaggtgct
    55621 cgaccatctg atgccgcatc tgctaagtga tgtctgtgcg cgtgaagacg ctgccgttac
    55681 gctgtcgcgc attaccgcct tgctggtggg gattgttacc cgcaccacct atttagaatt
    55741 gctcagtgaa ttccccgcgg cgcttaaaca tttgatttct ctgtgtgccg cgtcgccgat
    55801 gattgccagc cagctggcgc gttatccatt attgctggat gaattgctcg atccaaacac
    55861 cctttaccag ccgacggcga ccgatgccta ccgcgatgag ttgcgccagt atttgctgcg
    55921 cgtgccggaa gatgacgaag agcaacagct tgaggcgctg cgtcagttca aacaggcgca
    55981 gctgttacgc atcgccgcag cggatatcgc cggtacgcta ccggtgatga aagtgagcga
    56041 tcacttaacc tggctggcgg aagccatgat agatgccgtc gttcagcagg cgtgggttca
    56101 aatggttgcc cgctacggta agccgaatca cctgaacgaa cgcgaagggc gtggttttgc
    56161 ggtggtcggc tacggcaagc tgggcggctg ggagttaggc tacagttccg atcttgacct
    56221 tatcttcctc catgattgcc caatggatgc gatgactgac ggtgagcggg aaatcgacgg
    56281 gcggcagttt tatctgcgtc tggcgcaacg cattatgcat ctgttcagta cgcgtacctc
    56341 ttccggcatt ttgtatgaag tggatgctcg actgcgtccg tccggggcgg cgggaatgct
    56401 ggtgacatcc gcagaagcat ttgccgatta tcagaaaaac gaggcctgga cgtgggaaca
    56461 tcaggcgctg gtgcgtgcgc gtgtagtgta cggcgatccg cagctcaccg cgcactttga
    56521 cgcagtgcgt cgcgagatta tgacgctgcc gcgtgaaggt aaaactctgc aaacggaagt
    56581 gcgggaaatg cgcgagaaaa tgcgcgctca tctcggcaat aaacatcgcg atcgctttga
    56641 tatcaaagct gatgaagggg gaattaccga tatcgaattt attacccaat atctggtgtt
    56701 gcgctacgct catgaaaaac cgaagttaac gcgctggtca gacaacgtgc gtattctgga
    56761 actactggcg caaaacgaca ttatggaaga gcaggaagcg atggcgctga cccgtgctta
    56821 cactacgctt cgcgatgaac ttcatcatct ggcattacag gaattgccgg gccatgtgtc
    56881 ggaggattgc ttcaccgcag agcgtgaact ggtgcgggca agctggcaga agtggctggt
    56941 ggaagaatga agtatggtat tatcgcgcgc aaattttgaa tctctcagga gacaggaatg
    57001 aaagtaacgc tgccagagtt tgaacgtgca ggagtgatgg tggttggtga tgtgatgctg
    57061 gatcgttact ggtacggccc caccagtcgt atctcgccgg aagcgccggt gcccgtggtt
    57121 aaagtgaata ccatcgaaga acgtccgggc ggcgcggcta acgtggcgat gaatatcgct
    57181 tctctcggtg ctaatgcacg cctggtcggg ttgacgggca ttgacgatgc agcgcgcgcg
    57241 ctgagtaaat ctctggccga cgtcaacgtc aaatgcgact tcgtttctgt accgacgcat
    57301 ccgaccatta ccaaattacg ggtactttcc cgcaaccaac agctgatccg tctggatttt
    57361 gaagaaggtt tcgaaggtgt tgatccgcag ccgctgcacg agcggattaa tcaggcgctg
    57421 agttcgattg gcgcgctggt gctttctgac tacgccaaag gtgcgctggc aagcgtacag
    57481 cagatgatcc aactggcgcg taaagcgggt gttccggtgc tgattgatcc aaaaggtacc
    57541 gattttgagc gctaccgcgg cgctacgctg ttaacgccga atctctcgga atttgaagct
    57601 gttgtcggta aatgtaagac cgaagaagag attgttgagc gcggcatgaa actgattgcc
    57661 gattacgaac tctcggctct gttagtgacc cgttccgaac agggtatgtc gctgctgcaa
    57721 ccgggtaaag cgccgctgca tatgccaacc caagcgcagg aagtgtatga cgttaccggt
    57781 gcgggcgaca cggtgattgg cgtcctggcg gcaacgctgg cagcgggtaa ttcgctggaa
    57841 gaagcctgct tctttgccaa tgcggcggct ggcgtggtgg tcggcaaact gggaacctcc
    57901 acggtttcgc cgatcgagct ggaaaatgct gtacgtggac gtgcagatac aggctttggc
    57961 gtgatgaccg aagaggaact gaagctggcc gtagcggcag cgcgtaaacg tggtgaaaaa
    58021 gtggtgatga ccaacggtgt ctttgacatc ctgcacgccg ggcacgtctc ttatctggca
    58081 aatgcccgca agctgggtga ccgcttgatt gttgccgtca acagcgatgc ctccaccaaa
    58141 cggctgaaag gggattcccg cccggtaaac ccactcgaac agcgtatgat tgtgctgggc
    58201 gcactggaag cggtcgactg ggtagtgtcg tttgaagagg acacgccgca gcgcttgatc
    58261 gccgggatct tgccagatct gctggtgaaa ggcggcgact ataaaccaga agagattgcc
    58321 gggagtaaag aagtctgggc caacggtggc gaagtgttgg tgctcaactt tgaagacggt
    58381 tgctcgacga ccaacatcat caagaagatc caacaggata aaaaaggcta accggaaagc
    58441 ggttcacaga tctcgcttcg tacatggcag gcctattgac gctgttgttt tttcaaccta
    58501 gagtaaagga acaagggtaa gggaggattt ctcccccctc tgatgagttg ttagtaagtc
    58561 gggaaactta acagtaacaa cacaaccagt atgatgacga gcttcatcat aaccctttcc
    58621 ttatacaagg ccccttcttc gggaggggct ttcccgtttc agccccttgc tgccccccga
    58681 ttgttgatga gcgccggacc ccgcgcaatg ctttcccaga ggcaattttg ccttccccga
    58741 gcggtcacgc aaaacgctgc aacgtcctga gcatttgcac cttttagcgg aacgcagata
    58801 taaaaaatcc ccggcttttt gggccaggga tatgtgttaa tgaaaccttc ttgacgctga
    58861 ttttttttca acctaaagta aaggaacaag ggtgagggag gatttctccc ccctctgatt
    58921 ggctgttaat aagctgcgaa acttacgagt aacaacacaa tcagtatgat gacgagcttc
    58981 atcataaccc tttccttctg taaggccccc ttcttcggga ggggctttcc cgtttcagcc
    59041 ccctgctgac tccccgattg ttgatgagcg ccagacccca cgcaatgctt tctctgatgc
    59101 agttttgcct tccccgagcc gtcacgcaaa atgctgcaac aacctgatca tttgcacttt
    59161 ttagcggaac gcagatataa aaaatccccg gctttttggg ccagggatta ttcctggaag
    59221 agggtttact ctgctttttc ttcgacggga gttgttgagg ttaagggtga agtcaatgcc
    59281 gccagtgagc cgcctgaaac gccaatttca ttgagcaagg agtcaatcag cggtgcctgt
    59341 gtgcggtaag agagagcggc tgataatgct tgttccgcca ggtttccgcc ccccacatta
    59401 cccgtgtttg catcacccgc agcgccgcca cgattcagac catcgacctg aataatcttg
    59461 ataccgtcga ttgacttcat cggctcaacg gatttctcta ttaccgcagg cagcgcctgc
    59521 aacaaggcca gtttgaattt aaggctggtt tgttcatcag aaagtacgtt gatagcatcg
    59581 ttcagcgcac gttgtgcttc tgcttccgcc agaccctttt tacgtgtagc ttcggctaac
    59641 tcaacgatag ccgccgcctg catctctgcg gcttcttttt ctgctttcgc ccgcacggtc
    59701 agttcaaccg ctttggtttc tgcatcctgc gcggcagcga ttagggcaac ttgtttagca
    59761 cgatcggctt cggcagtctg gcgagtggtt tctacgtttt gttgggcgct taccacttct
    59821 gcaagtgcga gattagcacg cgcttctgcc tgggactgtt gttccgattt ggcggcaata
    59881 gcgatcgatt tcgtctggtt agcgatttcg gtgacctgct gctgttcgat ctctttaatg
    59941 cgaacttcac gttcagcttc aacctttctt gagcggacgg cctgttcgcg gtcgatttct
    60001 gtttcctgaa tctgtcgttc agccagaatt cgtgtctgct ctgcttcacg acgacgttca
    60061 gcttcaaaag ccgcaatacg tgcattctgt tcggcagtac gggttttaac ctgctgctcc
    60121 tgctcaagcg tcataaacgc ttcttgctgt tcaatctcca gcttgcgcga tagcgcatcg
    60181 cgattttttt cacgcaccgc aacttctaca tcctgttcaa cttcgttacg ttcgcggcga
    60241 cggcgctctg tttcctgagt cagtttggtt aaaccttcgg cgtcaaaggc attgttcgga
    60301 ttgaaatgtt ctttcgaggt ctggttaaag ttggtaagtg aaacgctctc cagttccaga
    60361 ccgtttttcg acaggtcttc tgccactgta ttttgcaccc cctgcacaaa gttctcgcgg
    60421 gtatcctgta actcatgcat ggtcatttgc gcagctgttg cacggagggc atcgacaaat
    60481 ttatcttcaa ccaacatacg taagtcttca ggcgacaggg tgcgttgccc cagcgtctgg
    60541 gcagcggtgg caatcccttc tactgaaggt tttacccgca caaagaaagc gactactaca
    60601 tcgacgcgca tacgatcttt cgtaatcagg ctatcaatgg ttgagcggct gacttccagc
    60661 ttcagagtat tcatattgat ggggattatt tcatgaaaga tcggcatcac gattgcgcca
    60721 ccgctcatta ccactttttg cccacctaaa ccagtacgaa caaaagcttg ctctgccgaa
    60781 gcgcgacgat agagcctggc gaaaataatt ccaataataa acagaatgca tacggcaata
    60841 attgcggtaa acatccagga gggcacagaa ttaacaatat catccattat taaatttcct
    60901 tttgattaat aaattacaat atttggggcc agggattatt ttccgccaaa tatcgcgttg
    60961 cagaaagtcg gcaaataatt aacactttgt ctcctttagt gaaaattttt ccttcttcag
    61021 gctccagtag taaataatgt atttggccaa attgatcggt gagttttcct tcgcagggat
    61081 tacctgaagt ggcttgatga ccggtaatta acgccatgct accaatatat tcttcttctg
    61141 taatagctga actatggtcg cgtggaatcc agggcgcaac tattttaccg gtataatgca
    61201 ccgcaataat cgtaaacagt aaactgacag gaaccacgaa caggttcgac agtggtgatt
    61261 gccagaccat gatgcaggca tgctgcaaca ggatgccaat aagaccaaaa aaaccagcca
    61321 gtaagcagag aacgacgaga gctggcaatc ttccaatatt gagataatga agtgcctggc
    61381 taatatgacc tgtggttata gaatcataat ggtcaagatg tgcatcaaga gcaccggaga
    61441 gcatgtgacc gcagataagc gcaaaaattt ctaacaggcc gatcaacagt acaaaggaaa
    61501 tagcgaaaag ataaggggtg ttatagtcgg cgaataaaat catatatcac tccaatgtcc
    61561 atttagtgac gcaatattta ctttgtaata gtttccttgc ttacatttat ctgtgttaat
    61621 aataacacag gttagagaag ttctggaaga gaaagtcttt taatatggtt ttttattttt
    61681 cactaaaaag tgtgatcggg gacaatatat ttacgcacgt tatgtttaaa ggcactacac
    61741 tgattgggga atactgaaat cagaaaaagc atatggcctt aaaaggtatg cgataaccgg
    61801 aggatgtgct tatggatcat agtcttaatt ctttaaataa tttcgatttc ctggcgcgta
    61861 gttttgccag aatgcacgca gaaggtcgcc cggtcgatat tctggccgtt actggtaaca
    61921 tggatgaaga acatagaacc tggttttgcg cacgttatgc ctggtattgt caacagatga
    61981 tgcaggcaag agagctggag ttagagcact gatataacgg gcctgatggc ccgttttagt
    62041 gtttgtatta gatactagtt ataggtgatt ttcaggatta cgtctttact aaacggtccc
    62101 tcaacaaccg tttctgccgg gttcgcactc agttctgccc tgaatggtgc ggtgaccgaa
    62161 ggcgcactag acgatgaaaa agaagcaaat tgtgagaagc ggttaaattc cgttgctgtt
    62221 ccatcgttat gaaatatctt caatagcaga ccattaccta tatccagatg gctataatca
    62281 ctcaaaatat ccgtagtgaa aaaactccct aaaatattaa actgctcagt acaggccgca
    62341 cccggatctt tggtcatgac tacgctaaac gtctcactca cgctggtatt tttaattgtt
    62401 cgggaattta ttttaccaaa gtcgacaacg ccatcagctg gttccagatc aacattaaca
    62461 aagcagtcaa ggacatgaat gttatttaat ccgcgaatcc tgaaggtcag atttttatct
    62521 acttctatag cacctgctcc gctcatacct ttaaattcaa gtgctatata ttcgtcaggt
    62581 aatacaagtt ttttatttac tttattggca tttttagcta gcttaatata tagctgcact
    62641 cgtactggaa aagttactga taattgcggt tcaggaccgc cactacagac tgcgggcttt
    62701 ttgtagccat tcccgaaggt tccggctcct tttatatcaa tacatacagg tgctgcaata
    62761 ggtagtggag atgtagaatc ataatcaacc ccattataag ttacaccgaa agtataagag
    62821 ttttgattat aaaagtcttg acctgatgct aacaacatca ttatatttgc ccatgcagat
    62881 gggtattctt ctcctggaga tgggcctggt agtgcattat tacagaaaac attcataaaa
    62941 tattcactgc tttgccagat tttatcacct tttttaccat cgctggggat agagattctt
    63001 ggtacgtaac caatgacaac ggtatttcca ccttcatgtt cagcatagca gtccattgcc
    63061 catgcaggta atgaactaaa acccataaaa aaagtaataa caattaacaa gtagcgcata
    63121 ttattgatct cggttggcta cagaaatatt acaaatgtta tttactacag ggcagtttat
    63181 ttcataaaaa cttaatccac caaagtcatt gatataaccc acctgaaaag ttgacggaac
    63241 atttttaaac tgcgtatttg catagctaaa aggggttacc attaagcttt gcgtttgtgg
    63301 taacatcgtt accccatttg ttccgatata acccagcgta acgtgataag gcatcttatt
    63361 gttaaagcgg atcgaaccgt catttctacg aatgatttca actttttcgt aagtcattac
    63421 cccatcgtct tctaattcaa tggcttttgg ccgccagaat agtttcaatg cgtgctgcat
    63481 agtgacttgc atcacatttt tttatctgtt ttgggcggta tttctcgcac gttaaaatag
    63541 aagagcgttt ctctgtcttt aggaagttga gcaatttttt cctgagttat cattcttatt
    63601 tgaatttgct cactgggatt taaacgcacc attggaggaa cagtgacaat aaagtcacgc
    63661 gatttattgc ctttcgtatc ttctatccat gtttgggcca gatagggcag gtttttactt
    63721 tgattagtaa gttttaatgc ttcgccttta tctgactcat tcattatgat acgggtacga
    63781 tccgggatga tggcacccca ggcaatgctg ctcatacagg agagtaatac tcctgctgca
    63841 atgatattct ttgtatttaa gtagcgcatt agtttcatcc tgaattaatt ttggctaata
    63901 cacggtaaaa tagccgttcc ggtggttaag ttgctgctat tgggtggttg aataaaacaa
    63961 gatttgtcac cccagcgtaa agcaagtttg ttatcatcct gaatgccgtt gaggtataca
    64021 aagccgccat cagcaaccag tcctaattct ttgtggctgg tttcatcttt tacgctaatc
    64081 cccagtggtg gatgactacc atctgccagg cgaataaccc ctatgatttg gtagcccttg
    64141 cgggtatcaa gtttggcgta gccaatagca ccctcggtta aggtggtggt gataaccgag
    64201 ttttcgatat ccacattttc tggcagattt cgggtatcaa cacttaatga tgttgttatg
    64261 taactgctga ctgatggaat aactccgatg ccataccgat tagttaccgc gcgtttattg
    64321 ttcaacggaa tatcttcagt gccgtctgcg tcgatcataa aacgcgagtc agcactaccg
    64381 ctatagtcgt ggaaagcggc accgtggcga gtcgcagtga atgaaccatt ccaactggca
    64441 ttcagagaat aataactgtc tcgctgtgat gtaccggaga ggtagagtcg accgttctcg
    64501 gtattatgct gataactggc gcgtagtgac gctttcatat ctttgaattc atcattgtcg
    64561 cctgaagcag aaacgctcca ggaattattt cgatcggaag agtcatacca cgaagcagta
    64621 tgcgaaatct cattatcctg atttcgttgc ataccatagc tcaatgttct actagttccc
    64681 cagggaatcg aaatatttag gtacagttga tcctggttgt cttcttccca gtttattcgg
    64741 ctaaatgtta gagaagtgga gacgtttttt aatggcccga tatcaaaatc gcggctaagt
    64801 gataatgaat aattcacatt actgctggcg tcccagtaag tattacgagc caaacttacg
    64861 tatgtattta accttaatga ttcaagatac tggttaaacg tgacaatata actctctttt
    64921 tcattctgat aattcgtgta atggttagtg tcattgatat attcattcat tgtcacaaag
    64981 tttttatcag agaaacggta accagcgaaa gtgagctggc taccggtaga ttcaaaacgt
    65041 tttgaatagt tagcgcggta
//
LOCUS       DBBGLQ010000024        63995 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.024, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000024 DBBGLQ010000000
VERSION     DBBGLQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.024"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            71..1735
                     /gene="tsr"
                     /locus_tag="ACIYSB_001891"
     CDS             71..1735
                     /gene="tsr"
                     /locus_tag="ACIYSB_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGC5428964.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMESASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKNVTPATPRKMAVADSGENWETF"
     gene            complement(1784..3145)
                     /gene="lgoT"
                     /locus_tag="ACIYSB_001892"
     CDS             complement(1784..3145)
                     /gene="lgoT"
                     /locus_tag="ACIYSB_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5428965.1"
                     /translation="MEKENIILDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(3360..4274)
                     /gene="lgoR"
                     /locus_tag="ACIYSB_001893"
     CDS             complement(3360..4274)
                     /gene="lgoR"
                     /locus_tag="ACIYSB_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGC5428966.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            4413..5435
                     /gene="lgoD"
                     /locus_tag="ACIYSB_001894"
     CDS             4413..5435
                     /gene="lgoD"
                     /locus_tag="ACIYSB_001894"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGC5428967.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIANLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            complement(5573..7864)
                     /gene="opgB"
                     /locus_tag="ACIYSB_001895"
     CDS             complement(5573..7864)
                     /gene="opgB"
                     /locus_tag="ACIYSB_001895"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGC5428968.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAQELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(8118..8612)
                     /gene="yjjA"
                     /locus_tag="ACIYSB_001896"
     CDS             complement(8118..8612)
                     /gene="yjjA"
                     /locus_tag="ACIYSB_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGC5428969.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            complement(8661..9398)
                     /gene="dnaC"
                     /locus_tag="ACIYSB_001897"
     CDS             complement(8661..9398)
                     /gene="dnaC"
                     /locus_tag="ACIYSB_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGC5428970.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            complement(9401..9940)
                     /gene="dnaT"
                     /locus_tag="ACIYSB_001898"
     CDS             complement(9401..9940)
                     /gene="dnaT"
                     /locus_tag="ACIYSB_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGC5428971.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            complement(10047..10520)
                     /gene="yjjB"
                     /locus_tag="ACIYSB_001899"
     CDS             complement(10047..10520)
                     /gene="yjjB"
                     /locus_tag="ACIYSB_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGC5428972.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            complement(10511..11281)
                     /gene="yjjP"
                     /locus_tag="ACIYSB_001900"
     CDS             complement(10511..11281)
                     /gene="yjjP"
                     /locus_tag="ACIYSB_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGC5428973.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNQDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTVAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            11900..12625
                     /gene="yjjQ"
                     /locus_tag="ACIYSB_001901"
     CDS             11900..12625
                     /gene="yjjQ"
                     /locus_tag="ACIYSB_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5428974.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            12583..13260
                     /gene="bglJ"
                     /locus_tag="ACIYSB_001902"
     CDS             12583..13260
                     /gene="bglJ"
                     /locus_tag="ACIYSB_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGC5428975.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSYLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(13298..14086)
                     /gene="fhuF"
                     /locus_tag="ACIYSB_001903"
     CDS             complement(13298..14086)
                     /gene="fhuF"
                     /locus_tag="ACIYSB_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGC5428976.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAIRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSTNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            14227..14463
                     /gene="yjjZ"
                     /locus_tag="ACIYSB_001904"
     CDS             14227..14463
                     /gene="yjjZ"
                     /locus_tag="ACIYSB_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGC5428977.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            complement(14502..14588)
                     /locus_tag="ACIYSB_001905"
     tRNA            complement(14502..14588)
                     /locus_tag="ACIYSB_001905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14552..14554),aa:Leu,seq:cag)
     gene            complement(14623..14709)
                     /locus_tag="ACIYSB_001906"
     tRNA            complement(14623..14709)
                     /locus_tag="ACIYSB_001906"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14673..14675),aa:Leu,seq:cag)
     gene            complement(14738..14824)
                     /locus_tag="ACIYSB_001907"
     tRNA            complement(14738..14824)
                     /locus_tag="ACIYSB_001907"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14788..14790),aa:Leu,seq:cag)
     gene            complement(15024..16055)
                     /gene="rsmC"
                     /locus_tag="ACIYSB_001908"
     CDS             complement(15024..16055)
                     /gene="rsmC"
                     /locus_tag="ACIYSB_001908"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGC5428978.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPEFDADKFWG
                     EYNVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            16158..16571
                     /gene="holD"
                     /locus_tag="ACIYSB_001909"
     CDS             16158..16571
                     /gene="holD"
                     /locus_tag="ACIYSB_001909"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGC5428979.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKISMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            16540..16986
                     /gene="rimI"
                     /locus_tag="ACIYSB_001910"
     CDS             16540..16986
                     /gene="rimI"
                     /locus_tag="ACIYSB_001910"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGC5428980.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            17001..17678
                     /gene="yjjG"
                     /locus_tag="ACIYSB_001911"
     CDS             17001..17678
                     /gene="yjjG"
                     /locus_tag="ACIYSB_001911"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGC5428981.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            17769..19358
                     /gene="prfC"
                     /locus_tag="ACIYSB_001912"
     CDS             17769..19358
                     /gene="prfC"
                     /locus_tag="ACIYSB_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGC5428982.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            19751..20356
                     /gene="osmY"
                     /locus_tag="ACIYSB_001913"
     CDS             19751..20356
                     /gene="osmY"
                     /locus_tag="ACIYSB_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGC5428983.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            20483..20644
                     /gene="ytjA"
                     /locus_tag="ACIYSB_001914"
     CDS             20483..20644
                     /gene="ytjA"
                     /locus_tag="ACIYSB_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGC5428984.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            20766..21839
                     /gene="yjjU"
                     /locus_tag="ACIYSB_001915"
     CDS             20766..21839
                     /gene="yjjU"
                     /locus_tag="ACIYSB_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGC5428985.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPASVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            21836..22615
                     /gene="yjjV"
                     /locus_tag="ACIYSB_001916"
     CDS             21836..22615
                     /gene="yjjV"
                     /locus_tag="ACIYSB_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGC5428986.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAEQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYGALGLHPGMLEKHSDASLEQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRLEPADEIAEVLLNNTYTLFNVL"
     gene            complement(22732..23595)
                     /gene="yjjW"
                     /locus_tag="ACIYSB_001917"
     CDS             complement(22732..23595)
                     /gene="yjjW"
                     /locus_tag="ACIYSB_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGC5428987.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIIDGKVAWSAAVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLNETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATQEDIEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(23567..25117)
                     /gene="yjjI"
                     /locus_tag="ACIYSB_001918"
     CDS             complement(23567..25117)
                     /gene="yjjI"
                     /locus_tag="ACIYSB_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HGC5428988.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPAL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLEDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEATANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            25375..26154
                     /gene="deoC"
                     /locus_tag="ACIYSB_001919"
     CDS             25375..26154
                     /gene="deoC"
                     /locus_tag="ACIYSB_001919"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGC5428989.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            26232..27554
                     /gene="deoA"
                     /locus_tag="ACIYSB_001920"
     CDS             26232..27554
                     /gene="deoA"
                     /locus_tag="ACIYSB_001920"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGC5428990.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            27606..28829
                     /gene="deoB"
                     /locus_tag="ACIYSB_001921"
     CDS             27606..28829
                     /gene="deoB"
                     /locus_tag="ACIYSB_001921"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGC5428991.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            28886..29605
                     /gene="deoD"
                     /locus_tag="ACIYSB_001922"
     CDS             28886..29605
                     /gene="deoD"
                     /locus_tag="ACIYSB_001922"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGC5428992.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKVLGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29766..30029)
                     /locus_tag="ACIYSB_001923"
     CDS             complement(29766..30029)
                     /locus_tag="ACIYSB_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5428993.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(30061..31077)
                     /gene="lplA"
                     /locus_tag="ACIYSB_001924"
     CDS             complement(30061..31077)
                     /gene="lplA"
                     /locus_tag="ACIYSB_001924"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGC5428994.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFTETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            complement(31105..31749)
                     /gene="ytjB"
                     /locus_tag="ACIYSB_001925"
     CDS             complement(31105..31749)
                     /gene="ytjB"
                     /locus_tag="ACIYSB_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HGC5428995.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LKELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            31855..32823
                     /gene="serB"
                     /locus_tag="ACIYSB_001926"
     CDS             31855..32823
                     /gene="serB"
                     /locus_tag="ACIYSB_001926"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGC5428996.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRSPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            32872..34254
                     /gene="radA"
                     /locus_tag="ACIYSB_001927"
     CDS             32872..34254
                     /gene="radA"
                     /locus_tag="ACIYSB_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGC5428997.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCNACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            34275..35507
                     /gene="nadR"
                     /locus_tag="ACIYSB_001928"
     CDS             34275..35507
                     /gene="nadR"
                     /locus_tag="ACIYSB_001928"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGC5428998.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            35541..35708
                     /gene="nadS"
                     /locus_tag="ACIYSB_001929"
     CDS             35541..35708
                     /gene="nadS"
                     /locus_tag="ACIYSB_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGC5428999.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(35815..37482)
                     /gene="ettA"
                     /locus_tag="ACIYSB_001930"
     CDS             complement(35815..37482)
                     /gene="ettA"
                     /locus_tag="ACIYSB_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGC5429000.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGSITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            37693..39630
                     /gene="sltY"
                     /locus_tag="ACIYSB_001931"
     CDS             37693..39630
                     /gene="sltY"
                     /locus_tag="ACIYSB_001931"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGC5429001.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            39720..40046
                     /gene="trpR"
                     /locus_tag="ACIYSB_001932"
     CDS             39720..40046
                     /gene="trpR"
                     /locus_tag="ACIYSB_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGC5429002.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(40193..40705)
                     /gene="yjjX"
                     /locus_tag="ACIYSB_001933"
     CDS             complement(40193..40705)
                     /gene="yjjX"
                     /locus_tag="ACIYSB_001933"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGC5429003.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     AQSPFHNAVY"
     gene            40757..41404
                     /gene="gpmB"
                     /locus_tag="ACIYSB_001934"
     CDS             40757..41404
                     /gene="gpmB"
                     /locus_tag="ACIYSB_001934"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGC5429004.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(41401..42270)
                     /gene="robA"
                     /locus_tag="ACIYSB_001935"
     CDS             complement(41401..42270)
                     /gene="robA"
                     /locus_tag="ACIYSB_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGC5429005.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            42481..42954
                     /gene="creA"
                     /locus_tag="ACIYSB_001936"
     CDS             42481..42954
                     /gene="creA"
                     /locus_tag="ACIYSB_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGC5429006.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            42967..43656
                     /gene="creB"
                     /locus_tag="ACIYSB_001937"
     CDS             42967..43656
                     /gene="creB"
                     /locus_tag="ACIYSB_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGC5429007.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     KQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLALTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            43656..45080
                     /gene="creC"
                     /locus_tag="ACIYSB_001938"
     CDS             43656..45080
                     /gene="creC"
                     /locus_tag="ACIYSB_001938"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGC5429008.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKKITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            45138..46490
                     /gene="creD"
                     /locus_tag="ACIYSB_001939"
     CDS             45138..46490
                     /gene="creD"
                     /locus_tag="ACIYSB_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGC5429009.1"
                     /translation="MLKSPLFWKMTSLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(46550..47266)
                     /gene="arcA"
                     /locus_tag="ACIYSB_001940"
     CDS             complement(46550..47266)
                     /gene="arcA"
                     /locus_tag="ACIYSB_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGC5429010.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            47362..47502
                     /gene="yjjY"
                     /locus_tag="ACIYSB_001941"
     CDS             47362..47502
                     /gene="yjjY"
                     /locus_tag="ACIYSB_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGC5429011.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            47902..48588
                     /gene="yjtD"
                     /locus_tag="ACIYSB_001942"
     CDS             47902..48588
                     /gene="yjtD"
                     /locus_tag="ACIYSB_001942"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGC5429012.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSDLRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDATADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            48802..48867
                     /gene="thrL"
                     /locus_tag="ACIYSB_001943"
     CDS             48802..48867
                     /gene="thrL"
                     /locus_tag="ACIYSB_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGC5429013.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     misc_feature    48803..48922
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            48949..51411
                     /gene="thrA"
                     /locus_tag="ACIYSB_001944"
     CDS             48949..51411
                     /gene="thrA"
                     /locus_tag="ACIYSB_001944"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGC5429014.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            51413..52345
                     /gene="thrB"
                     /locus_tag="ACIYSB_001945"
     CDS             51413..52345
                     /gene="thrB"
                     /locus_tag="ACIYSB_001945"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGC5429015.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPETA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            52346..53632
                     /gene="thrC"
                     /locus_tag="ACIYSB_001946"
     CDS             52346..53632
                     /gene="thrC"
                     /locus_tag="ACIYSB_001946"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGC5429016.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQETRNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            53846..54142
                     /gene="yaaX"
                     /locus_tag="ACIYSB_001947"
     CDS             53846..54142
                     /gene="yaaX"
                     /locus_tag="ACIYSB_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGC5429017.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHLHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(54295..55071)
                     /gene="yaaA"
                     /locus_tag="ACIYSB_001948"
     CDS             complement(54295..55071)
                     /gene="yaaA"
                     /locus_tag="ACIYSB_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGC5429018.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(55141..56571)
                     /gene="yaaJ"
                     /locus_tag="ACIYSB_001949"
     CDS             complement(55141..56571)
                     /gene="yaaJ"
                     /locus_tag="ACIYSB_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGC5429019.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFTLLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            56850..57803
                     /gene="tal"
                     /locus_tag="ACIYSB_001950"
     CDS             56850..57803
                     /gene="tal"
                     /locus_tag="ACIYSB_001950"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGC5429020.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            57918..58505
                     /gene="mog"
                     /locus_tag="ACIYSB_001951"
     CDS             57918..58505
                     /gene="mog"
                     /locus_tag="ACIYSB_001951"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGC5429021.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(58540..59106)
                     /gene="satP"
                     /locus_tag="ACIYSB_001952"
     CDS             complement(58540..59106)
                     /gene="satP"
                     /locus_tag="ACIYSB_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGC5429022.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(59255..59968)
                     /gene="msyB"
                     /locus_tag="ACIYSB_001953"
     CDS             complement(59255..59968)
                     /gene="msyB"
                     /locus_tag="ACIYSB_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGC5429023.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(59994..60398)
                     /gene="yaaI"
                     /locus_tag="ACIYSB_001954"
     CDS             complement(59994..60398)
                     /gene="yaaI"
                     /locus_tag="ACIYSB_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGC5429024.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGAIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            60775..62691
                     /gene="dnaK"
                     /locus_tag="ACIYSB_001955"
     CDS             60775..62691
                     /gene="dnaK"
                     /locus_tag="ACIYSB_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGC5429025.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            62780..63910
                     /gene="dnaJ"
                     /locus_tag="ACIYSB_001956"
     CDS             62780..63910
                     /gene="dnaJ"
                     /locus_tag="ACIYSB_001956"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGC5429026.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     ERQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT        15658 a        15922 c        17197 g        15218 t
ORIGIN      
        1 cgcgggatat tcataaagtt tttgctttcc aggccgaaaa tcttgcatcg gtccacagga
       61 aagagaaacc atgttaaaac gcatcaaaat tgtgaccagc ttactgctgg ttttggccgt
      121 ttttggcctt ttacaactga catcaggcgg tctgttcttt aatgccttaa agaatgacaa
      181 agaaaatttc actgttttac aaaccattcg ccagcagcaa tccacgctga atggcagctg
      241 ggtcgcgttg ttgcagacgc gtaacaccct caaccgcgcg ggtatccgct acatgatgga
      301 tcagaataat attggtagcg gttcaaccgt tgctgagctg atggagagtg ccagtatttc
      361 gctgaaacag gcggaaaaaa actgggcgga ttacgaagcg ttgccgcgtg acccgcgtca
      421 gagcaccgcc gcagcggcag agatcaaacg taattacgat atttatcaca atgcgctggc
      481 ggagctgatc caactgttag gtgcaggcaa aatcaacgag ttctttgatc agccgaccca
      541 gggatatcag gacggtttcg agaagcagta tgtggcttac atggagcaaa acgatcggct
      601 ctatgatatc gccgtcagcg ataataatgc ctcctacagc caggcgatgt ggattctggt
      661 gggcgtgatg atcgtcgtac tggcggtcat cttcgccgtc tggttcggta ttaaagcctc
      721 gctggtagcg ccaatgaatc gcctgattga cagcattcgt catattgcag gcggcgatct
      781 ggtgaaaccg attgaggtgg atggctctaa tgagatgggg caactggcag agagtttgcg
      841 ccatatgcag ggagagctga tgcgtaccgt cggtgatgtg cgcaacgggg ccaatgccat
      901 ttatagcggt gccagcgaaa tcgccaccgg caataacgat ctctcttcgc gcaccgagca
      961 acaggccgct tcgctggaag agacggcagc cagcatggag caactgaccg caacggtgaa
     1021 acagaacgcc gaaaatgcgc gccaggccag ccacctggcg ttaagtgctt ctgaaacggc
     1081 gcaacgcggc ggcaaagtgg tagataacgt ggtgcagact atgcgcgata tctccaccag
     1141 ttcgcagaaa atcgccgata ttatcagcgt aattgacggc attgccttcc agaccaatat
     1201 tctggctttg aacgcggcgg ttgaagcagc gcgcgcgggt gagcaagggc gcggttttgc
     1261 ggtggtcgcg ggtgaagtgc gtaatctggc tcagcgcagc gctcaggcgg ctcgtgaaat
     1321 taaaagcctg attgaagact cggtggggaa agtagatgtt ggctctacgc tggtcgaaag
     1381 cgcaggggaa acaatggcgg agattgtcag tgccgtgacc cgcgtgacgg acattatggg
     1441 cgaaattgct tctgcttctg atgagcagag ccgtggtatc gatcaggttg gcttagcggt
     1501 tgctgagatg gaccgggtaa ctcaacagaa cgctgcgctg gtggaagaat ctgccgctgc
     1561 cgccgccgcg ctggaagagc aggccagtcg cctgaccgaa gctgtggcag tgttccggat
     1621 tcagcaacag cagcaacagc agcgtgaaac atcggctgtg gtaaaaaacg tgacgccagc
     1681 tacgccgcgt aaaatggcag tggcagatag cggggagaac tgggaaacgt tttaatcgcc
     1741 ataaaaatgc ccgataagca aaatgttatc gggcataggg agtttaatct ttacgcgggt
     1801 cgttgatcgg ctgacgaacc aggaagatgt acgccagtgc tcccgctgcg gtgacgcaac
     1861 cgcagatgat tagtgccaga cggaatgagt gggtggtatc aacaataaaa ccagtaatga
     1921 tcggcgcaaa agaggcgcag atgaagctgg caaagttctg gatactgccc accgacgccg
     1981 tcatgcgaga agcaactgcg acgtggatca agccccagca ggatgttccg gcaaagtgaa
     2041 tacagaacag tgccatgcca atcagcagaa ccgccgtcat ggatgttgtc gcttgcggta
     2101 cgaccagcgt aaaggcggca gaacagaaca tcccggcaat aatgcagatc ttacggcttt
     2161 taatcggagc cattccccct ttgaccagcc agtcagtaac gtaaccgttg accagcatcc
     2221 cggcagcccc aaacaggaaa gggatagccg ccatcaaccc tgtgcttttt aaatccaggt
     2281 tataggctgt ttgcaggtaa ccaggaagcc aggccagata cagccacgca gtgtagttga
     2341 tgccactgaa tccgagcatc attccccaca ttgtgcggtt acggaacagg ctgcgccatt
     2401 cggcaaaact gagcggatct cggcgggcat tgacgctacc tgcattgaga taagcttgtt
     2461 caacggcagt cagttctacg tgctcgcggt tgcgatagag catataccag ccaatggcga
     2521 gaaaaatccc cagcacacca atggtaataa acatcccacg ccagcccatc accagcatca
     2581 tcgccgccag aatcggtggg cttacggcaa cgccaatggt agaagctgcg ttgaagaagc
     2641 ccatcgggcg tccgcgctct ttgatgttga accagtcgtt aatgactttt acgccacatg
     2701 ggttcatcgg cgcttcacca atccccatac cgatacgcac caacacgaac tgcgtaaagc
     2761 tgtgcaccat gccagacatg gcctggaaca gtgaccagaa gaacatcccc agtcccagca
     2821 tcaggcgtgg gcctttacga tccaatagtg ggccgcaagg aagttgcgca atcccgtaag
     2881 cgagtgaaaa cacggagagc aaagcgccga tttcggtggc acttaatccc aattcttcac
     2941 gaatcgttaa atttgctacc gacagcgaac tgcggtcgag ataattgatt accgccgcaa
     3001 aaaataataa caacatggcg gtggtttgaa tgcgtttaat tcgggtactg cgttgaacta
     3061 atccatctgg tggcacggga atatctgccg acgaagatgg agtaaatgaa gaacgcggat
     3121 cgaggatgat attttctttt tccacgccgg actcctgtat ttataatttt aattgtttac
     3181 gtaattgtgc attacgcaag gttgacatta acggcaggtt aaagttttag gactttacta
     3241 ccgctcacaa aaacattttt agtcgactga ttgtgagcat aggtaagcgg gaatttcccg
     3301 gtatttatct tttgataaaa ccgtgcaccc gctaatagtt tgctgttttt aatccgtact
     3361 taatgagcat aacgcgtgtt ctcattaatg gatcgaatca ttgattgttt agctgaattc
     3421 aaatggttac ggagcgcaag ggtggcatca aggtcactgc ggcaaatcag agcgctgaga
     3481 atggtcatat gctcatcgac ggcaatgata ttgcgctgtt taagatcgct ttcgtcccat
     3541 tgatagtgaa aatgaaagat gactgagatg atctcaagag attgatcaaa aaagatattg
     3601 tcggcagctg aaagcagtaa ggagtgaaaa tctctgtcaa gttgcgagaa catgcgaaag
     3661 ctattgccga tgttgtcacg cagtagccga tgacgttcga gcatggtttt cgcctgcagc
     3721 cagcggggat catgatcagg caggttgaga aagtgctgaa gtgaatgtgt ttcgagcatt
     3781 tcgcgtagtt caaacagctg ctcggcgtag gattgatcga attgcttcat gctccactgg
     3841 ccgcgctttt cgctttgaat aagattataa cggccgaatt ttaaaagata ttctcttact
     3901 acaaccgggc tgacacccgc ggcccgagcc agttgcagtt cagaaaaagt ttccccaggg
     3961 cgtaactggc gctggtttat catcgtgaaa aaagcctgct caaacacttt gttttgttca
     4021 ctcattgaag cggtagtaca ggcgaaaccg tcatcatggt caggtttacg cgcaatcaca
     4081 tagtcgtttc caacctgcgt taagacgccg cattcgcgta agtggctgag aatatgacgc
     4141 actgtagtgc ggctgatgtt gtacatttcc gccagcgcac tttgcgatgg aagtggggaa
     4201 gggatatgac cccgcgccat atcatcaatg acctggttaa tcacattgtg gcgtaaattt
     4261 tgtgaacgac tcatattttc ctccttgtga gtccattaaa acccgattta aaacattttt
     4321 atgcgtgtgt tttcacaaaa cagattttcc gttccgctgc catttttcat tttcattgat
     4381 atctaaggca acaaaaaatg gagagccgaa tcatgtctac gatgaatgtt ttaatttgcc
     4441 agcagccgaa agaattagtc tggaaacaac gcgagatacc tattccgggt gacaatgaag
     4501 cattaataaa aattaagtct gtcgggattt gcggtaccga tattcatgcc tggggtggaa
     4561 atcaaccatt ttttagttat ccacgggttt taggccatga aatatgtggg gagattgttg
     4621 ggctgggtaa aaatattgca aatcttaaaa atggacagca agtagcggtg atcccttatg
     4681 ttgcctgtca gcaatgcccg gcgtgtaaaa gcgggcgtac caattgctgt gaaaaaattt
     4741 cagtcattgg cgtgcatcag gatggcggtt ttagtgagta tttgagcgtg ccggtggcga
     4801 acattttgcc cgcagatggt attgacccgc aggcggcagc attgattgaa cctttcgcta
     4861 ttagcgctca tgcggtacgt cgcgcagcca ttgctcccgg cgagcaggtg ctggtggtcg
     4921 gggcggggcc aatcggtctg ggcgcggcgg caatcgctaa agccgatggc gcacaggtgg
     4981 tggtggcgga taccagtccg gcgcgccgtg aacatgtggc aacgcgtctg gagttgcccg
     5041 tgctggaccc atcagccgaa gattttgacg cgcagctgcg ggcgcagttt ggtggttctc
     5101 tggcgcagaa agtgatcgac gcgacaggta atcaacatgc gatgaataac accgtgaatc
     5161 tgattcgtca cggcggcacg gtggtatttg tcggtctgtt taaaggtgag ttgcagttct
     5221 ccgatccaga attccataaa aaagaaacga cgatgatggg tagccgcaac gccacgccgg
     5281 aagatttcgc taaagtcggt cgactgatgg cggaagggaa aatcactgcc gacatgatgt
     5341 taacccatcg ctacccgttc gccacgctgg cagaaaccta cgagcgtgat gtgattaaca
     5401 atcgtgagtt gattaagggt gtaattactt tctgataagg cgagattatt aaagttgcca
     5461 tgcagcgtcc ggggaagtgt tgggcgctgt tttttttgtt tcttaatagt gtgttgtaag
     5521 ccggagaagg cgtgtaggtc gcaccttatg cgacctacac atcagtcagg aattatcctt
     5581 cacgttctac cactttaatt tctaccatgc cgatcccgag cttacgcggc gagtgtccga
     5641 ggatattccc ttcgttggtt gagacaggtt ccggcggcac aattaccagc gtgtcggcat
     5701 cggttgggtt atcgaaatgc agcgtggtgg tggtcacttc attgcccagc acaagagttt
     5761 gttcttcatt gcctacgcgt accggaatag gacggctggc gttattgccg tatgctttgg
     5821 cggtaatcac caggtcaaat ttcttcggca gcggatgctt gtactcgatt tttacttcgt
     5881 cgcccagttg cgcgttggac cagcggcccc acgactccgg acgggaaatc ccgctaaact
     5941 gtttcacctc ttctggcgca ccggcgacgt taaagatgaa actgtcggct ttgtagcgaa
     6001 tatcgttatc aacaatcttc agcgtatcga cattgccttt gtaacgcgcc atgtcgatga
     6061 ccgtatcttt aaatgccgtt ttgcccttcc acattgtttt atcaacatgc tggacaattt
     6121 gctgaccgcc aagctgccct tgcgagacac accagtcggt ggagagtgcc agttcctgag
     6181 cccaaagttg tgccatcttg tagcaacggt caacccagac gaaattgtcg cgtggagcga
     6241 aatcggccag ctggaaacgc agcggtgctg agtattcgct ttccggcagc ggttccacgc
     6301 gtttgtctga aacccgcaac agcagcggca aacggaaatg gctacccgag aaggcaatca
     6361 tgtttttctg ctggtcgatg gtgaactctt tcatctcttt agggaatttc cacaggcgga
     6421 tgatatccgg cttccacgcc aatgttttct ctttgatatt gaggaagatt tctgacattg
     6481 actgcccgga taaactgcta cgaccaagtc cgagatagtt atcgccaccg agaatgtcca
     6541 gcaccgtcgc gccgttatcc atcgtgttac gcttcaccgc cagcgtctct tgctgcggct
     6601 tgtcgccacg gatgacaaaa aacaggttat tgcgatcctg tttattaagg tatttccacg
     6661 ccgtgttgtt catcgctaaa tggtcagaag agacgacgat gacggtatct ttaaaccacg
     6721 gtgacgcttt gattttgttg ataaacgtcg cgatattctc ctgactgcaa cttaccgcgc
     6781 tgaatgactg attaggctta ccgtcaaaat catatttttt gcggttacag gtgcgcgaga
     6841 taaaaccatc cgggtgatgg gtatcgaccg tcagggtaaa cagtgagaat cgctgaccag
     6901 aacgggaaag ctcttcaaac tttttccacg cttcatcgag aacggtatcg tcgtagaatc
     6961 cccagtcgtt gcgatagtgc gggtcggcca ccacgctttt cagctcttct gagccgtata
     7021 agtgatcgaa gccgtgcgat ttcaggaaca catctttacc tgcaaaacgc agatttgcgc
     7081 cctgcacgaa atagttctga taacccgagt ttttcaggat atctcccaga cagatgttct
     7141 gcgggaagaa gctggagaca gaggcggagg cgttgccttc aaagggggca aacagcggta
     7201 tgccgcactg agaagccacc atgcccgcaa tcgtgtaatc cgttcctggc agctgctgcg
     7261 tgtggctgaa atccaggcct tcatttttca acgcgcccag ttcaggcgtg agatccggga
     7321 aagcctcgtt atcaaaatag gtccgctcga gactttcgcc gtagatataa accaggttga
     7381 gtttcgggtc agggatagtt ttcgacggct ctttataata agccgcaaag tccgggtcgc
     7441 cgtcgcgtga ctgggatttc actagttccg ttatctgacg aaatgccggg ctggcatcca
     7501 ctgaacccag cgccagtaag agtgccagca ggctgtaacc aaaatggtgc ggatgatggc
     7561 gacgacggcg caggatccag cccagcgcac cgaacaccgc cgtcagcccc agcacaatgc
     7621 cgataccagg caggatgtat ttactgacac cagcaccggt caggctgttg gttaaggtat
     7681 agagaaccgc gtcgttaata ccatcgccag taaaatagtc gctggcaaac agggtgatat
     7741 ttaaaacgac aaatagcccc agtaccgtta atgtggctgc aaaccaccag gtgttacgtc
     7801 ccgctttcca tgcgtaaatc agcacagagg cgagaaaaag ggcgaaagag agtagttctg
     7861 acaacgggcg atcctcacta aaccgggccg tttagccaac gtttaagctt tttgagaagc
     7921 acaatgtaat ggcgtcgcca tattgctgca attctagtgt caaaaaaatg cgatgttgtt
     7981 aggaattagt ttataaacag acttttttga ttacggtcgc ttcccgtgta acagggggaa
     8041 aaccgtccag gagcagggaa aagggcagga gagagccgcg tcagccgggt aacagctaaa
     8101 acgcggcgtt tcgcggttca ggaaatgaag ttcagcccct gctttaacac cagatcgcag
     8161 gctttggttt tcaccttttc tgccagcgga gtcgttccga tattatccag attgagttgc
     8221 tgaccatctt tagttttcag caaaccctga atgccatcca gataattggt gtcttctttt
     8281 tgctcttcac tgttcaggcc cagtttttcc agtacctgat tcttgatgtt ttcggcatcg
     8341 gttaccgaag ccagcttttg cttcgcgcag tattgcagaa tgcctgcggc attgttcatg
     8401 ttatctgcgc ttaaggcctg gtttccgctg ctaagcaagt tagttaatga cgcgagcgac
     8461 caaccgcctt cctgtgtcgt gctgttttgg ttgccaagtt cgctggcggc gctggagagc
     8521 gcatctttcc aggacgcagc atgcaccccg gtggaaatta atgcgctggc ggcaatggca
     8581 cagcacagaa gatgtttaac agttttcatc atcattactc aaggtggaat tgtgtcgcag
     8641 tataccgcca gaaacgggga ttaatactct ttacccgtta cccggctacg gtagctatcc
     8701 cagttgaaga tcacccacaa actgttaccc aggcgcatac ggtccatcac gcgttcgccc
     8761 agcaacttgg tcatctcttc catattactg ttggtcagca tcccggttgg gcgtttggaa
     8821 gaagagcggc gatcgacgat ctggttgatg atcacttttt cgtatttcga ttcggtttgc
     8881 acgccgatct catcgatcac cagcagatca acattgctca gatcgttaag cagttgttct
     8941 tcgctggtac cgctattcct gaaggtatct ttcatcgccg acataatatc ggccacggtg
     9001 ataatcaata cggatttacc gcgtagcagc agctcgttgc agattgccgc cgccagatgg
     9061 tttttgccgg ttcctggctt accagaaaag atgaagctgg cgatgttgcc gtcaaactct
     9121 tcgacatact ggcgcgcttt gcttaacgca ttcatctgcc cttcacactc aacgcgatag
     9181 ttctcaaagg agcagttctg atgcagtgga cgaataccgg agcggttaaa ggtgcgctgc
     9241 attttcatcg cccgattttc acgttcgaga gcggcggagc ggatcgcccc ttgttctttc
     9301 tgccaggcca gtagttcttc acccgttttg aaagcaggtt tgatatgggc aggcatcatt
     9361 ttttgcaggc gttgcatcag gtcgccaacg tttttcatcg ttaccctctg aatcctggtg
     9421 gaatttggct gtcaggttca ctgaccgtat tcacatctcg tttcggcagt ccgccgttgc
     9481 tggcacgacc gatttgcagg ctgcgcgcca gtttttgttg ccactgcaca tggtgaaaga
     9541 ctttaccttc cgcctgccag taggcaatga atgaggccag ttcttcggtg gtcaccggct
     9601 ctcttagcgc cacgccccat agcgccgcca ggcggataaa gtcggcatcg ggttgccagt
     9661 ccggatacat ggcgaatttc cccatgggta cagctacggg agcagcttgc ggttcctgat
     9721 agagttgatc gtccagagcg acatcgcttc ccggacgcgc cagcttttct tccagcgcca
     9781 gcagttcagc caggcgtgca ggcgtgacgg cataaaacgc cggggcattg ttagcaaata
     9841 cggcaaccac accgccttca gcttttgcca gaacggtttg gtgatcgtgt accagggcgt
     9901 caataccaac gacgtccggg gtcaaaactc tggaagacat actgtttctc aaatatggaa
     9961 cgatgaacga gaagggagat aacttttata gtaacacacg gaggagagga ggctgtctct
    10021 catccgtgtg cttaagcgag gcaattttat acgcgagggc gcttgcggta caaccataat
    10081 ccaggaatgg aaagaccgat ggataacgca ccaacaattg atgaagctgt aagaaagttg
    10141 gttaacaggg taatcatcaa cggttcgctg taacctagct ggctgatttt taccgccgaa
    10201 atcattgcgg tataagccga tatgccaggg aacataggaa taacagccgc cacggtaaac
    10261 actttcggat gcgccagata ccagcgcgac cattgaatac caatggtgcc gaccagcata
    10321 gaagccataa aagttgacca ctcaatattc agcccgctgg tcattaagat cattcgggaa
    10381 ccatgaccta tcgagccaag cagcgcacac cagcgtaacg cccgcacggg aacgttgaac
    10441 accatcgcaa agccgaccgc aggaatggcg gcgaggatca tatcctgcgc caacgctaac
    10501 agaaattcga tcacacccat ccgcgtagcc cccaaatcgt cagtgccatc accacgccga
    10561 cgcaggtagc cagtgtcagc agactggcga tcgcccagcg tgccagtccg gtattgatgt
    10621 ggcctttaaa catatcggcg acggcattaa tcaacggaaa acccggcacc agcagcagaa
    10681 cgctggcggc catcgcgatg gtgggtgtat tgctgaaagt gggaagttgc aaaagcaaac
    10741 cggaaatggt ggtggcggcg aaagcggtga gacaaaagtt gatctgtgga tgaagatgac
    10801 gttgtgccag cagctggcgg atatacatcg cgaccgtact ggcaaagaag gtgatgacgg
    10861 caccatccca gccaccttta ttgagtttac agaaacaggc gcaagaaagg ccgaccatta
    10921 aggctaccag ccatctcggg taacgtaatg gctgaatttg gctaaatcgt ttctctacac
    10981 ctttgtaatc cagcagatga tgctccgcca gaatcacaat gtgctggact tcagtcacca
    11041 catgcatatt aatgccgcga tcttgatttt ttcgtgtcga tgtcaggcat tgcccatctt
    11101 taatagttgt cagcactatg gcgttcgaag agatagaact ttcgacgctg tccattccca
    11161 gcgcccgacc cagtcgtgag gaaagctcat caaccaacgc gctttccgca ccatgttgca
    11221 aaagaaataa tccacactgg atacaaagcc gtgttacggc tcgctgttgc tcagtttgca
    11281 taactcgccc tttgtcatag acgtgctgcg tattgttcag ataatgagaa tccatttgta
    11341 tcatgaagca cggttgtttt gcggtaggtt aagatcaaaa ttgcgctgat taatcctggt
    11401 tatcgggaga agctcggtga taaaaagcga attatctctc gtcgcaaata aattatcaca
    11461 tgaatattat cactataatg aatttattgt ttagccttta cgaatcagga taaaagataa
    11521 ctgggcgcgt tttttaattt ttacaagtta aaactatcag attttattga ataaatattg
    11581 attgtgtgat tttgcattct actggaaggg atatcagtta attataaatt aaatgaatga
    11641 gaattctgtt tttcattaag tatttctaac aacttaatgt agatatttta aatgtctcca
    11701 ggctatttca ctaggttatc atttgatgct aaccttctga cacatgcagt ggagttgttg
    11761 tgcagcagga gtatgctgat atgaaagtga atgctaagga taatttattc gcttaatcta
    11821 ttaatttgct ggaaacattt aaggaatgaa tatcctgaat aaaccatcat aatttttaga
    11881 cagtggatgt ggaggaaata tgttgccagg atgctgcaaa aatggaattg ttatcagtaa
    11941 aatacctgtt atgcaagcag ggttaaaaga ggtcatgagg actcacttcc ctgaatatga
    12001 aataatatcc agcgcctctg cggaggacct taccttatta caattacgtc gttccggatt
    12061 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
    12121 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gatcctggta
    12181 ttcccaggca gtagaattgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
    12241 cattgagaat ctggtcaaga ccgtacgttc cggtaatacg cacgcagagc gtatcagcgc
    12301 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
    12361 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
    12421 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
    12481 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
    12541 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
    12601 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
    12661 ttattcagaa agtttgcggg taacccttat aagctccata cctataccag tcaggagtca
    12721 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
    12781 agaagtgagc gcagagaggg attatcttac ctgactgaac tggcgattaa gtttccgcgt
    12841 acccggcgtt tagttattgc ggatgatgat attgaagctc ggctgattgg ttcattgtcg
    12901 ccatcaccgt tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
    12961 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
    13021 attaatcaaa gccgaacgct aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
    13081 ggctactcaa tgacacaaat tgccgagcag cttaaacgca atatcaaaac gatccgtgca
    13141 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
    13201 gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
    13261 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatcgccac
    13321 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
    13381 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc attttctcaa
    13441 aaaagagggc atggcgcagc gattcaaccg tcgcctcgcc aagcagctgt ttcatctcag
    13501 tcagatacca gttgatcaaa taaccggtat tgctccagat aagtttgccg ttgatctctc
    13561 ccgttgcttc taatgcctgc actaccggaa ccagcgcctg actgattaac gtttccattc
    13621 gctgctgcgg cgaatgtagt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
    13681 cggcgcgtcc ggtttcgtga aactcaacgt ggaaatgttc cggcgacaca tctaatgctt
    13741 tttcctgcgt cagcagcgcc aacattaatg gtggcaccat caggccgata taccattgcg
    13801 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
    13861 gatcggaata gaccgccagt agagaactta gcgcattcgt tgatgaccat tgcgccagcg
    13921 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctcgagcaaa tgttcacgat
    13981 gttcggcgat cgtcgcccgt atggcttgtg caagcccagc atcctgcggc tggagatgcg
    14041 ttcgccagat aacatcttca tagagcggtg cggaacgata ggccataatc gggatagtaa
    14101 tctaaatgat aatgattgct aatcctagcg ataggtttac ccgatagcaa gggatttctc
    14161 tggcttgcaa atgataaaaa ttatcatatg atattagtta tcattatcaa tgaaagagat
    14221 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
    14281 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
    14341 ggattaatat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cgttttgctg
    14401 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
    14461 tgaaaaatca gggaagaaac aagaaaggaa gtaaagataa ttggtgcgag gggggggact
    14521 tgaaccccca cgtccgtaag gacactaaca cctgaagcta gcgcgtctac caattccgcc
    14581 accttcgcac agtcatctta ctttttttga tatcgcctcg tttggtgcga ggggggggac
    14641 ttgaaccccc acgtccgtaa ggacactaac acctgaagct agcgcgtcta ccaattccgc
    14701 caccttcgcc cagtgcgagc aatatcaacg tggtttttgg tgcgaggggg gggacttgaa
    14761 cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat tccgccacct
    14821 tcgcatacca tcaattctta aaaagaattg ctaccacgga ggcgcattct agtggttttc
    14881 agcttttcgt caatagttaa ttatcgccgg aggtgtaatt gctggaaaaa tgtccatcag
    14941 cggattgtgg ataattcgtt tgtcggtcag agaaggcgtg tacgccgcat ccgacacggt
    15001 atgcggcgac agatatcaga tacttaacct ttcttcgcct ggcgggtcat aatggcgcga
    15061 tacaccttga agcgcccggt ttgcgcgatc acttcgtgga agccaaatgt ctcatccagc
    15121 acgtccggat aaggcaggaa ggcgttcgct acaattcgca gctcgccgcc gctattaaga
    15181 tgacgcaccg cgccgcgaat cagcgtttgc gccgcatcga ggctggtttg catcccatcg
    15241 tggaacggcg ggttggagat gatcatatca aaacgacctt tcacctcgga aaagacgtta
    15301 ctggcaaaga cttcaccttc aacaccgttg gccgcaagtg ttgcgcggct ggcttctacc
    15361 gctggcgcag agacatcgca cagagtgaga cgaattttcg gcgaatggcg cgcaaaggca
    15421 acagaaagca cgcccgcgcc acagccgaca tccagcactt tacctttcgt gtgcggagtt
    15481 aacgtcgaga gcagtaactg gctaccgaca tccagaccgt cgcggctaaa cacgcccggc
    15541 agcgttttga ccgtcaggcc atccacgtta tattcgcccc agaatttatc cgcatcaaat
    15601 tccggctgtt tttccagacg accaaaatag aggccgcagc gacgggcgct gtcgacttta
    15661 ttcaacggcg cataatctgc cagcatctgt tcggcgctgc gcacgccgct gcggttctcg
    15721 ccaacgacaa aaatatccgt gcccaccggc agcagagaaa gtaaattcat caactggaac
    15781 tgggcttccg gtttattctt cggccagtag tagatcagcg tatcgcaatc tgcgacgtca
    15841 tctgccgttg cgaccagact aaaacgggcg ttatccccca tctggcggct taacacctgc
    15901 caatggtgga attgctgggt atgagcacgg ctggccgcgg tatctaaacg cgcgggcagg
    15961 tcatcctgta agtctccggc aaacagaata cggctttgtt cgaaatcatc actgtgacgc
    16021 agcaagactt cacttgccgg ggtaaatgca gacatggaat gctcctcaat tgatactggc
    16081 ggcgattata gccatatgtt ggcgcggtat cgacgaattt gctatatttg cgcccctgac
    16141 aacaggagcg attcgctatg acatcccgac gagactggca gttacagcaa ctgggcatta
    16201 cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga gattgccatt gcgatcccgg
    16261 cacacgtccg tctggtgatg gtggcaaacg atcttcccgc cctgactgat cctctggtga
    16321 gcgatgttct gcgcgcatta accgtcagcc ccgatcaagt gctgcaactg acgccagaaa
    16381 aaatctcgat gctgccacaa ggcagtcgct gcaacagctg gcggttgggt actgacgaac
    16441 cgctatcact ggaaggcgct caggtggcat caccggcgct caccgaatta cgggcaaacc
    16501 caacggcacg cgccgcgtta tggcaacaaa tttgcacata tgaacacgat ttcttccctc
    16561 gaaacgactg atttaccagc ggcttaccac attgaacaac gcgcccacgc ctttccgtgg
    16621 agtgagaaaa cctttgccag caaccagggc gagcgttatc tcaactttca gttaacgcaa
    16681 aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc tggatgaagc tacattgttc
    16741 aatattgcgg tcgatcctga ctatcagcgt cagggattgg gaagggcgct gctggaacat
    16801 ctgatcgacg aactggaaaa acgcggcgtg gcgacgctat ggctggaagt ccgtgcctca
    16861 aacgctgccg ccattgcact gtacgaaagt ttaggcttta acgaggcgac gattcgccgc
    16921 aattactacc ccaccacgga cggtcgcgaa gacgccatca tcatggcgtt gccaatcagt
    16981 atgtaagaca aggtggaata atgaagtggg actggatttt ctttgatgcc gatgaaacgc
    17041 tgtttacctt tgactcattc accggcctgc agcggatgtt tcttgattac agcgtcacct
    17101 ttaccgctga agattttcag gactatcagg ccgttaacaa gccactgtgg gtggattatc
    17161 aaaacggcgc gatcacttca ttacagcttc agcacgggcg gtttgagagc tgggccgaac
    17221 ggctgaacgt cgagccgggt aaactcaacg aagcctttat taatgcgatg gcggaaatct
    17281 gtacgccgct gccgggcgcg gtttctctgc ttaacgccat tcgtggcaac gctaaaattg
    17341 gcatcatcac caacggcttt agcgccttgc agcaagtgcg tctggaacgc acgggcctgc
    17401 gtgattattt cgatttgctg gtgatttccg aagaagttgg cgttgccaaa ccgaataaga
    17461 aaattttcga ttatgcgctg gaacaggcgg gcaatcctga ccgttcacgc gtgctgatgg
    17521 ttggcgacac tgccgagtcc gatattctcg gtggcatcaa cgccgggctt gcgacctgct
    17581 ggctgaatgc gcacaatcgc gagcaaccag aaggcatcgc gcctacctgg accgtttctt
    17641 cgttgcacga actggagcag ctcctgtgta aacactgatt gcctcccccc cgttgatggg
    17701 taaaatagcc gcaatttttc gttttcaaca agcgcggcgc gatgccgctt actcaagaag
    17761 aaagaattat gacgttgtct ccttatttgc aagaggtggc gaagcgccgc acttttgcca
    17821 ttatttctca cccagacgcc ggtaagacta ccatcaccga gaaggtgctg ctgttcggac
    17881 aggccattca gaccgccggt acagtaaaag gccgtggttc caaccagcac gctaagtcgg
    17941 actggatgga gatggaaaag cagcgtggga tctccattac tacgtctgtg atgcagtttc
    18001 cgtatcacga ttgcctggtt aacctgctcg acaccccggg gcacgaagac ttctcggaag
    18061 atacctatcg taccctgaca gcggtggact gctgcctgat ggttatcgac gccgcaaaag
    18121 gtgttgaaga tcgtacccgc aagctgatgg aagttacccg tctgcgcgat acgccgatcc
    18181 tcacctttat gaacaaactt gaccgtgata tccgcgaccc gatggaactg ctcgatgaag
    18241 ttgagaacga gctgaaaatc ggctgcgcac cgatcacctg gccgattggc tgcggcaagc
    18301 tgtttaaagg cgtttaccac ctttataaag atgaaaccta tctttatcag agcggtaaag
    18361 gccacaccat tcaggaagtc cgtattgtta aagggctgaa taacccggat ctcgacgctg
    18421 cggttggtga agatctggcg cagcagctgc gtgatgaact ggagctggtg aaaggcgcgt
    18481 ctaacgagtt cgacaaagaa ttgttccttg cgggcgaaat cactccggta ttctttggta
    18541 ctgcgctggg taacttcggc gtcgatcata tgctggatgg tctggtggag tgggcacctg
    18601 cgccgatgcc gcgtcagact gatacccgta ccgtagaagc gagcgaagac aaatttaccg
    18661 gcttcgtatt taaaattcag gccaacatgg acccgaaaca ccgcgaccgc gtggcgttta
    18721 tgcgcgtggt atccggtaaa tatgaaaaag gcatgaaact gcgccaggtg cgcaccgcga
    18781 aagatgtggt gatctccgac gcgctgacct ttatggcggg cgaccgttcg cacgttgaag
    18841 aagcgtatcc gggagatatc ctcggcctgc acaaccacgg caccattcag atcggcgaca
    18901 cctttaccca gggtgagatg atgaagttca ccggtattcc gaacttcgca ccagaactgt
    18961 tccgtcgtat ccgcctgaaa gatccactga agcaaaaaca gctgctcaaa gggctggtac
    19021 agctttccga agagggcgca gtgcaggtgt tccgtccgat ctccaacaac gacctgatcg
    19081 ttggtgcagt tggtgtgctg cagtttgatg tggtggtagc gcgcctgaag agcgaataca
    19141 acgttgaagc agtgtatgag tcagtcaacg ttgccactgc acgctgggta gaatgtgcgg
    19201 acgcgaagaa attcgaagag ttcaagcgta agaacgaaag ccaactggcg cttgatggcg
    19261 gcgataacct cgcttacatc gctaccagca tggtcaacct gcgtctggcg caggaacgtt
    19321 atccggacgt tcagttccac cagacccgcg agcattaatt cttgcctcca gggcgcggta
    19381 gccgctgcgc cctgtcaatt tcccttcctt attagccgct tacggaatgt tcttaaaaca
    19441 ttcacttttg cttatgtttt cgctgatatc ccgagcggtt tcaaaattgt gatctatatt
    19501 taacaaagta atgacatttc tgacggcgtt aaataccgtt caatgcgtag atatcagtat
    19561 ctaaagccgt cgattgtcat tctaccgata ttaataactg attcagaggc tgtaatggtc
    19621 gttattcatc acccatcgct tttgtgatgg cgaccattga cttctgtaga gggtgaagtc
    19681 tctccctatt cagcaatgca acctcgtgtt gccaggctca aattacgagc aaacatacag
    19741 gaataaatcg atgactatga caagactgaa gatttcgaaa actctgctgg ctgtaatgtt
    19801 gacctctgcc gtcgcgaccg gctctgccta cgcggaaaac aacgcgcaga ctaccaatga
    19861 aagcgcaggg caaaaagtcg atagctctat gaataaagtc ggtaatttca tggatgacag
    19921 cgccatcacc gcgaaagtga aggcggcgct ggtggatcat gacaacatta agagcaccga
    19981 tatctctgtc aaaaccgatc aaaaagtcgt gaccctgagc ggtttcgttg aaagccaggc
    20041 tcaggccgaa gaggcagtga aagtggcgaa aggcgttgaa ggggtgacct ctgtcagcga
    20101 caaactgcac gttcgcgacg ctaaagaagg ctcggtgaag ggctacgcgg gtgacaccgc
    20161 caccaccagt gaaatcaaag ccaaactgct ggcggacgat atcgtccctt cccgtcatgt
    20221 gaaagttgaa accaccgacg gcgtggttca gctctccggt accgtcgatt cccaggcaca
    20281 gagcgaccgt gctgaaagta tcgccaaagc ggtagatggt gtgaaaagcg ttaaaaatga
    20341 tctgaaaact aagtaattcg tcgtaattcg tcctcccgaa ctttgtcggg aggcgcgatg
    20401 tgcaccacac taaaaatatc gcattgtgtg attttcacgc tcatatcaag cggtgacatt
    20461 aactatggta aaggagacgc ttatgtttcg ttggggcatc atatttctgg ttatcgcgtt
    20521 aatcgccgcc gcacttgggt ttggtggtct ggccggtacc gctgcaggcg cagctaaaat
    20581 tgtctttgtc gtcgggatta ttctgttcct ggtgagtttg ttcatgggcc gaaaacgacc
    20641 ctagatttca gaccattaag aaaattttat ccaaagccag tccagcggac tggcttttgc
    20701 ggttttagcg aataataaat tgcgttactt tgtcattctt tcacaacaaa aacaggaagg
    20761 cagaggtggg gcagcgaata cctgtaacgc ttggtaatat tgcgccgttg tcgctaaggc
    20821 cgttccagcc tggacggatt gctctggtgt gcgaaggcgg cggacagcgt ggaattttca
    20881 cggctggcgt gctggatgag tttatgcgtg cgcagtttaa tcctttcgat ctttatctcg
    20941 gcacatctgc cggggcgcag aacctctcgg cgtatatctg caatcagccc ggttacgcgc
    21001 gtaaagtcat catgcgctat accacaaaac gcgaattttt cgatccattg cgctttgtcc
    21061 gtggaggaaa tcttatcgat ctcgactggc tggtggaggc caccgcaagc cagatgccgt
    21121 tgcaaatgga caccgccgcg cggttgtttg acagcggcaa atcgttttat atgtgcgcct
    21181 gtcgtcagga tgactacgcg ccgaattact ttttaccaac caaacaaaac tggctggatg
    21241 tgattcgcgc ctccagtgcg atacctggct tttatcgtag cggagtgtcg ctggaaggca
    21301 ttaactacct ggatggcggg atcagtgatg cgattccggt taaagaggcg gcaaggcagg
    21361 gcgctaaaac gttggtcgtc atccgcaccg tgccgtcgca aatgtactac acgccgcagt
    21421 ggttcaaacg catggaacgc tggctgggtg acagtagcct gcagccgctg gtcaatctgg
    21481 tgcagcatca tgaaaccagc tatcgtgaca ttcagcaatt tattgagaaa ccaccgggca
    21541 aactgcggat attcgaaatt tatccgccga agccattgca tagtatcgcg cttggcagtc
    21601 ggattccggc gctgcgtgaa gactataaac ttgggcgttt atgcggtcgt tattttctgg
    21661 caacggtcgg taagttattg actgaaaaag cgccgcttac ccgccatctg gtgccggtgg
    21721 tgacgccgga gtcgatcgtt attccgcctg cgtcagtcgc caacgatacg ctggttgccg
    21781 aagtgatcga cgctccgcag gcgaacgacc cgacatttaa caatgaggat ctggcttgat
    21841 ttgccgtttt atcgacaccc actgccattt tgatttcccg ccgtttagtg gtgatgaaga
    21901 ggccagcctg caacgcgcgg aacaagcggg cgtaggcaag atcattgttc cggcgacaga
    21961 ggcggaaaat tttgcccgcg tgcaggcgtt agcggaaaaa tatcaaccgc tgtatggcgc
    22021 gttagggttg catccgggga tgttagaaaa acatagcgat gcatcgctgg agcagttaca
    22081 gcaggcgctg gaaaggcatc cggcgaaggt ggtggcggtg ggggagatcg gcctggatct
    22141 ctttggcgac gatccgcaat ttgagaggca gcagtggtta ctcgacgaac aactgaaact
    22201 ggcgaaacgc tacgatctgc cggtgatcct gcattcacgg cgcacgcacg acaaactggc
    22261 gatgcatctt aaacgccacg atttgccgcg tactggcgtg gttcacggtt tttccggcag
    22321 cctgcaacag gctgaacggt ttgtacagct gggctacaaa attggcgtag gcggcactat
    22381 cacctatcca cgcgccagta aaacccgcga tgtcatcgca aaattaccgc tggcatcgtt
    22441 gttgctggaa accgacgcgc cggatatgcc gctcaacggt tttcaggggc agcctaaccg
    22501 cccggaacag gccgcgcggg tgtttgctgt gctttgtgag ttacgcttgg aacccgcaga
    22561 tgaaattgcc gaagtgttgc ttaataacac gtatacgttg tttaacgtgc tgtaggccgg
    22621 ataaggcgtt cacgccgcat ccggcagttg gcgcacaatg cctgatgcga cgcttgacgc
    22681 gtcttatcat gcctacaagt ctgtgccgaa ctgtaggcca catccggcat gtcacaaata
    22741 cagcgccgga aatatcaacc ggctcacccc gcgcaccttt aacgcatcag ccaacggctc
    22801 aatgtcttcc tgcgtggcgc tcggccagct ttgcgcctcg ccatacacgc cgtgggcatg
    22861 aaacgcgttc aggcgtaccg gaacatcgcc aagtcccttg attaacgccg ccagttcttc
    22921 gatgtgttgc aaataatcca cctggccagg gattaccagc aaacgcagtt ccgccagctt
    22981 accgcgctct gccagcaaac agatgctgcg cttgatctgc tgattatcgc gtccggttaa
    23041 ttgctgatga cattcgcttc cccacgcttt gagatcgagc attgcgccgt cacacaccgg
    23101 gagcaatttt tcccagccgg tttcgttcaa catgccgtta ctgtccacga ggcaggtgag
    23161 atggcacagt tgcggatcgt ttttgatggc agtaaacagc gccaccacaa acggcaactg
    23221 ggtcgtggct tcgccgccgc ttaccgttat cccttcaata aacagcaccg ctttgcggac
    23281 atggttaagc acctcgtcca cgctcatgga ttgcgccatt ggcgtggcat gttgcgggca
    23341 catcttcagg caggtatcac attgctcgca aacagccgcg ctccacgcca ctttgccgtc
    23401 gataatctgc aacgcctgat gcggacactg cggcacgcac tctccgcaat cattacagcg
    23461 ccccatcgtc cacggattgt gacagttttt gcagcgcaga ttgcagccct gcaaaaacag
    23521 agccagacga ctgcctggcc cgtcaacgca ggagaagggg ataatcttac tgactaaagc
    23581 gcatctgctg ttcatggctt atcacgcgcg gctgacgttc cagaatacga gtgttgcgtg
    23641 cggcttcttc tcccagccag gtagtgttgg tgcgtgaacc ttcggcgcga tatttttcta
    23701 aatccgacaa acgcaccata taaccggtaa cgcgaaccag atcgttaccg ctgacattgg
    23761 cggtaaattc acgcattccg gctttaaagg caccgaggca aagctgtacc agcgcctgcg
    23821 gattacgttt gatggtttcg tcgagcgtca gaatgtcgct gatgccggaa taataataag
    23881 catgatgcgg cgcgacagtt tgcagatggg taatcggatc tggctcatcg ccatatggta
    23941 aacgcgcgcc cggcgtggtg ccgatatcgg aactgatccc cgactgtgcg tgtaacatgg
    24001 cgcgtttttg ccagccatat ttcacagggg tattggcgac aaactccgcc agttgcgcgc
    24061 tgatgcgata acccacttca tttgcggtgg cttctttacc gtaacgcgcg gcaattcctt
    24121 ctttttcaca cagcaagtta accgcttccg ccagcccata catgccaaac attggcacaa
    24181 aacgttcagg gttaatcagc ccttctttca ccaggaagct attctcaaag aagtgtgatt
    24241 gttgatagag gaattcacac cgcgcatcga tgatggcgat ctgctgttgg cagtagtgcg
    24301 gtagagtgcg cgtaaagaag tcctccagcg attcactgcg ctcggcaatg gctttcaggt
    24361 ttaggcgtac cagcgtgctg ccaccacccg ccagcggcag tgagttgtaa cagctcacaa
    24421 tcccgtagcc cccttttgtg aaaattttat catgcaccgg accgttggcg atgtgcggtt
    24481 tgctacattc acagatgttc ttcgccactt ccagcagcag gtcatcaggg gtgatttcag
    24541 gatcgtagat aaaggtcagg ttcggtgaaa cctgcttcaa ctctgcgtct gcacgtaaga
    24601 ttgcgcgggt aatgggcgaa tcagacgggc cgatattggc gtgcataaag gcatctggca
    24661 gggttctgtc gaggtaacgc cagaaacgtt ttattcgaat atcgatctcg tcttgtgtta
    24721 gaattctaac atacggttgc aacaacgcat ccagttgccc caggtagacc ggcatcgatg
    24781 tgaccgacgg tacgtggtgg taaagaatgg tcagcagaga gagtgcgtca tcaagatctt
    24841 tcgcgccttc cagctccagc cattcggaac cgttcgccag aaaacgggcg taatcgggta
    24901 agacatagcg cggtttgtac ggcgcatgac cttcaaacat atcgcagatt acaccctcat
    24961 ccagcgcgcg gcgggcttcg gcaggcaacg cggggtaagg cagattgttt tctgcttcca
    25021 gtgccagaaa atggcgcttc tgctccgggc taagtaccgg gctggtgaca atttgctggc
    25081 aacgttgttg cagtgcattt tcatgagaag tgggcatctt cttttccttt tatgccgaag
    25141 gtgatgcgcc attgtaagaa gtttcgtgat gttcactttg atcctgatgc gtttgccacc
    25201 actgacgcat tcatttgaaa gtgaattatt tgaaccaaat cgcattacag tgatgcaaac
    25261 ttgtaagtag atttccttaa ttgtgatgta catcgaagtg tgttgcggag tagatgttag
    25321 aatactaaca aactcgcaag gtgaatttta ttggcgacaa gccaggagaa tgaaatgact
    25381 gatctgaaag caagcagcct gcgtgcactg aaattgatgg acctgaccac cctgaatgac
    25441 gacgacaccg atgagaaagt gatcgccctg tgtcatcagg ccaaaactcc ggtcggcaat
    25501 accgccgcta tctgtatcta tcctcgcttt atcccgattg ctcgcaaaac tctgaaagag
    25561 cagggcaccc cggaaatccg tattgctacg gtaaccaact tcccacacgg taacgacgac
    25621 atcgaaatcg cgctggcaga aacccgtgcg gcaatcgcct acggtgccga tgaagttgac
    25681 gtggtgttcc cgtaccgcgc gctgatggcg ggtaacgagc aggttggttt tgacctggtg
    25741 aaagcctgta aagaggcttg cgctgcggcg aacgtactgc tgaaagtgat tattgaaacc
    25801 ggcgaactga aagacgaagc gctgatccgt aaagcgtctg aaatctccat caaagcgggt
    25861 gctgacttca tcaaaacctc taccggtaaa gtggctgtga atgcgacgcc ggaaagcgcg
    25921 cgcatcatga tggaagtgat ccgcgatatg ggcgtagaaa aaaccgttgg tttcaaaccg
    25981 gcgggcggcg tgcgtactgc ggaagatgcg cagaaatatc tcgccattgc agatgaactg
    26041 ttcggtgctg actgggctga tgcgcgtcac taccgctttg gtgcttccag cttgctggca
    26101 agcctgctga aagctctggg ccacggcgac ggtaagagcg ccagcagcta ctaaggcttg
    26161 atttgccgga tgatgcagcg atgctcatcc ggttccccga ttacctcgtc tcttttccac
    26221 gggaggttac cttgtttctc gcacaagaaa ttattcgtaa aaaacgtgat ggtcatgcgc
    26281 tgagtgatga agaaattcgt ttctttatca acggcattcg cgacaacact atctccgaag
    26341 ggcagattgc cgccctcgcg atgaccattt tcttccacga tatgacaatg cctgagcgtg
    26401 tctcgctgac catggcgatg cgagattcag gaaccgttct cgactggaaa agcctgcatc
    26461 tgaatggccc gattgttgat aaacactcga ccggcggtgt cggcgatgtg acttcgctga
    26521 tgttggggcc gatggtcgct gcctgtggcg gctatattcc gatgatctcc ggtcgcggcc
    26581 tcggtcatac tggcggtacg ctcgacaaac tggaatccat ccctggcttc gacattttcc
    26641 cggatgacaa ccgtttccgc gaaattatta aagacgtcgg cgtggcgatt atcggtcaga
    26701 ccagctcact ggctccggcg gataaacgtt tctacgcgac ccgtgatatt accgcaaccg
    26761 tggactccat cccgctgatc actgcctcga tcctggcgaa gaaactggcg gaaggtctgg
    26821 atgcgctggt gatggacgtg aaagtgggta gcggcgcgtt tatgccgacc tacgaacttt
    26881 ctgaagccct tgccgaagcg attgttggcg tagctaacgg cgctggcgtg cgtaccaccg
    26941 cgctgctcac cgatatgaat caggtactgg cctccagtgc aggtaacgcg gttgaagttc
    27001 gtgaagcggt gcagttcctg acgggtgagt atcgtaaccc gcgtctgttt gatgtcacaa
    27061 tggcgctgtg cgtagagatg cttatctccg gcaaactggc gaaagatgac gccgaagcgc
    27121 gcgcgaaatt gcaggcggtg ctggacaacg gtaaagcggc agaagtcttt ggtcgtatgg
    27181 tagcggcaca aaaaggcccg actgacttcg ttgagaacta cgcgaagtat ctaccgacag
    27241 cgatgctgac gaaagcagtc tatgctgata ccgaaggttt tgtcagtgaa atggataccc
    27301 gtgcgctggg gatggcagtg gttgcaatgg gcggcggacg ccgtcaggca tctgacacca
    27361 tcgattacag cgtcggcttt actgatatgg cgcgtctggg cgaccaggta gacggtcagc
    27421 gtccgctggc agttatccac gcgaaagacg aaaacagctg gcaggaagcg gcgaaagcgg
    27481 tgaaagcggc aattaaactt gccgataaag caccggaaag cacaccaact gtctatcgcc
    27541 gtatcagcga ataacggtat actgatctga tcatttaaat ttgaagcact gagtacggag
    27601 aacatatgaa acgtgcattt attatggtgc tggactcatt cggcatcggc gctacagaag
    27661 atgcagaacg ctttggtgac gtcggggctg acaccctggg tcatatcgca gaagcttgtg
    27721 ccaaaggcga agctgataac ggtcgtaaag gcccgctcaa tctgccaaat ctgacccgtc
    27781 tggggctggc gaaagcacac gaaggttcta ccggtttcat tccggcggga atggacggca
    27841 acgcggaagt tatcggcgcg tacgcatggg cgcacgaaat gtcatccggt aaagataccc
    27901 cgtctggtca ctgggaaatc gccggtgtcc cggttctgtt tgagtgggga tacttctccg
    27961 atcacgaaaa cagcttcccg caagagctac tggataaact ggtcgaacgc gctaatctgc
    28021 cgggttacct cggtaactgc cactcttccg gtacggtcat tctggatcag ctgggcgaag
    28081 agcacatgaa aaccggcaag ccgattttct atacctccgc tgactccgtg ttccagattg
    28141 cctgccatga agaaactttc ggtctggata aactctacga actgtgcgaa atcgcccgtg
    28201 aagagctgac caacggcggc tacaacatcg gtcgtgttat cgctcgtccg tttatcggtg
    28261 acaaagccgg taacttccag cgtaccggta accgtcacga cctggctgtt gagccgccag
    28321 caccgaccgt gctgcagaaa ctggttgatg aaaaacacgg ccaggtggtt tctgtcggta
    28381 aaattgcaga catctacgcc aactgcggta tcaccaaaaa agtgaaagcg actggcctgg
    28441 acgcgctgtt tgacgccacc atcaaagaga tgaaagaagc gggtgataac accatcgtct
    28501 tcaccaactt cgttgacttc gactcttcct ggggccaccg tcgcgacgtc gccggttatg
    28561 cggcgggtct ggaactgttc gatcgccgtc tgccggagct gatgtcttta ctgcgcgatg
    28621 acgacatcct gatcctcact gctgaccacg gttgtgatcc gacctggacc ggtactgacc
    28681 acacgcgtga acacattccg gtactggttt acggcccgaa agtaaaaccg ggttcattgg
    28741 gtcaccgtga aaccttcgcg gatatcggcc agactctggc aaaatatttt ggtacttctg
    28801 atatggaata tggcaaagcc atgttctgat ggatttgggc ggagcgttga ctccgccttt
    28861 gttatgtcac aaaaaggata aaacaatggc taccccacac attaatgcag aaatgggcga
    28921 tttcgctgac gtagttttga tgccaggcga cccgctgcgt gcgaagtata ttgctgaaac
    28981 tttccttgaa gatgcccgtg aagtgaacaa cgttcgcggt atgctgggct tcaccggtac
    29041 ttacaaaggc cgcaaaattt ccgtaatggg tcacggtatg ggtatcccgt cctgctccat
    29101 ctacaccaaa gaactgatca ccgatttcgg cgtgaagaaa attatccgcg taggctcctg
    29161 tggcgctgtt ctgccacacg taaaactgcg cgacgtcgtt atcggtatgg gtgcctgcac
    29221 cgattccaaa gttaaccgta tccgttttaa agaccatgac tttgccgcta tcgctgactt
    29281 tgacatggtg cgtaacgcgg tagacgcggc taaagtactg ggcgttgatg ctcgcgttgg
    29341 taacctgttc tccgctgacc tgttctactc tccggacggc gaaatgttcg acgtgatgga
    29401 aaaatacggc atcctcggcg tggaaatgga agcagctggt atctacggcg tcgctgcaga
    29461 atttggcgcg aaagccctga ccatctgcac cgtgtctgac cacatccgca ctcacgagca
    29521 gaccactgcc gctgagcgtc agaccacctt caacgacatg atcaaaatcg cactggaatc
    29581 cgttctgttg ggcgataaag agtaattgtg tttcgctgaa aggcgattgc cttgtgaagc
    29641 cggagcggga gactgctccg gctttttagc atctattcat caatagcaac tgatttatag
    29701 atttactatt atttttcata tggatagaaa ataaaatcca ttcaagtatc tattcacgaa
    29761 tctattcatt tatgttcagc cgtcatccag gaagggcata tcagcaactc tggtggacaa
    29821 ccataaattc ctgccatttt gcttttgatg tttgaactaa gccgttccgt cttttcgatc
    29881 tgttttagag aattaatgga gatacctaac attttactta cttcagcaag tgatagttta
    29941 tgcctgatac gcacggcggt tagccaggat ataccctggc tgaacattaa attgataatc
    30001 tcccgttcct ggttttccag atcgatcata tttccttccc tggatattta aatcagtgag
    30061 ttaccttacc gcccccgcta tccacgtcga caactcccgt agctcttttt cctgttccgg
    30121 gaagtcaacc aacagcgctt cgcactcctg ttgcagcata tccgcgcggt acaggcagcc
    30181 ttgcagtcgc ccggcgagag cttccagagg tgcggggttg aggctgtcgg taaacacctg
    30241 ggcgcgggtg atatggcctt tttcaacgtc gaaatgcagt tccacgccgc cccaggtaaa
    30301 gcgttcatcc agcagatgcg agaatgccgg agcctgaccg aagttccatt cccagctact
    30361 ctggcgggca aaggtttcgg tgaagtttgg caagtctggc gttttgtccg gggagatgat
    30421 ttccgcttcc acgcgctcgc cataatgggc gaaaaaggcc tcggttatgg cctcgcaaac
    30481 ctgctcatgg gtgatccccg gcagcaattc ggtcagattg gtcacgcggg aacgtaccga
    30541 cgtaatgcct ttcgccgcca gtttcttttt atccgggttg agatagtttg ccagacggct
    30601 aaggtcagca ttgagtagca aggtgccatg atggaagccg cgatctttag tttcgcgata
    30661 ggctgagcct gagactttgc ggtcgccttc ggcggttttc accaccagat cgttacggcc
    30721 ggacgcttcg gcgctgacgc cgagcgcgtt cagagcattg agcacaatcg acgtggagat
    30781 agttttatcg tactccggct tgccagccat aaaggtaaag caggtattac cgagatcgtg
    30841 gaacaccgcg ccgccaccgc tgctgcgccg cgccaggcgg acgttatctt cttccatccg
    30901 ccgggtatta cactctttcc acgggttctg tgcgcgacca atcaccacgg tgtcggcatt
    30961 gcgccagaga aacagaacgc gctgcgtggc gggcatctgg cgaaaaatac actcttccac
    31021 cgccaggtta aaccacgggt cgtaagagtc agagatgagc aggcgtaatg tggacataac
    31081 gatttccttt cttgtagtaa tgggtcactc ttttttctcg ctttcttcct cttccggcac
    31141 cggtttgctg gcggttaaca ggaagggcga ttgctgccag cgggtgcgtt taccctgtag
    31201 cagcgtgcgg gtcagcacta cgccgattgc cagtgagagc agcaacatca ggcgtaaaat
    31261 gttagtggtg ttatccacct gttgggcttc ggtggcgagc gtatgggtgt cgagcgtcag
    31321 gcgcagatag ccgagcggtc cgtttttacc cgcaattggc tcgacaatct gctggttaaa
    31381 atagccgccc gcttttttac cgtcgagcgc caaccggtcg cgcacttcga cgctttcgcc
    31441 agaacgggcg ataagatcgc cttgttcgtc atacacgccc gcgtcgagga tgcggctttc
    31501 atccgttaac tgatcaagga tcgcctgaat acgtttttca tccggtgagt cggtacgcat
    31561 cagcggtgca acattcagcg ttacctgacg cgccagggtg cgggccagtt ctttcagctg
    31621 tggattacgc tgtcgctggt ggttttgact aaaccatgac gccccctgca tcagcgccac
    31681 taacaaggcg agacagaaca ggacaatcac tgcccgatgc agccggaatt tcagttttgt
    31741 gcgagccatc ttccaccctt tgaaaatttg agacttaatg ttgccagaag caatggatac
    31801 aaggtagcct catgcgttat tttccctgct tcgaacgatt ttacaggagc cttaatgcct
    31861 aacattacct ggtgcgacct gcctgaagat gtctctttat ggccgggtct gcctctttca
    31921 ttaagtggtg atgaagtgat gccactggat taccacgcag gtcgtagcgg ctggctgctg
    31981 tatggtcgtg ggctggataa acaacgtctg acccaatacc agagcaaact gggcgcggct
    32041 atggtgattg ttgccgcctg gtgcgtggaa gattatcagg tgattcgtct ggcaggttca
    32101 ctcaccgcgc gggctacgcg cctggcccac gaagcgcagc tggatgtcgc gccgttgggg
    32161 aaaatcccgc acctgcgctc tccgggtttg ctggtgatgg acatggattc caccgccatc
    32221 cagattgaat gtattgatga aattgccaaa ctggccggaa cgggcgagat ggtggcggag
    32281 gtaaccgaac gggcgatgcg cggcgaactc gattttaccg ccagcctgcg cagccgcgtg
    32341 gcgacgctga aaggcgctga cgccaatatt ctgcaacagg tgcgtgaaaa tctgccgctg
    32401 atgccaggct taacgcaact ggtactcaag ctggaaacgc tgggctggaa agtggcgatt
    32461 gcctccggtg gctttacttt ctttgctgaa tacctgcgcg acaagctgcg cctgacagcc
    32521 gtggtagcca atgaactgga gatcatggac ggtaaattta ccggcaatgt gatcggcgac
    32581 atcgtagacg cgcagtacaa agcgaaaact ctgactcgcc tcgcgcagga gtatgaaatc
    32641 ccgctggcgc agaccgtggc gattggcgat ggagccaatg acctgccgat gatcaaagcg
    32701 gcagggctgg ggattgccta ccatgccaag ccaaaagtga atgaaaaaac ggaagtcacc
    32761 atccgtcacg ctgacctgat gggggtattc tgcatcctct ctggcagcct gaatcagaag
    32821 taattgcctg cccgtcatcc tgcgggcggc acagcattaa cgaggtacac cgtggcaaaa
    32881 gctccaaaac gcgcctttgt ttgtaatgaa tgcggggccg attatccgcg ctggcagggg
    32941 cagtgcaacg cctgccatgc ctggaacacc atcaccgagg tgcgtcttgc tgcgtcgcca
    33001 acggtggcgc gtaacgagcg tctcagtggc tatgccggta gcgccggggt ggcaaaagtc
    33061 cagaaactct ccgatatcag ccttgaggag ttgccgcgtt tttcaaccgg atttaaagag
    33121 ttcgatcgcg tattaggcgg tggcgtagtg ccaggaagcg ccattctgat tggcggtaac
    33181 ccaggcgcgg ggaaatccac gctgctactg caaacgctgt gcaaactggc gcagcagatg
    33241 aaaacgctat atgtcactgg cgaagagtcg ctgcaacagg tggcaatgcg cgctcatcgc
    33301 cttggcctgc cgactgacaa tctcaatatg ttgtcggaaa ccagcatcga gcagatctgc
    33361 ctgattgccg aagaagagca accgaagctg atggtaattg actccattca ggtgatgcat
    33421 atggcggatg tacagtcatc gcctggcagc gtggcgcagg tgcgtgaaac ggcggcttat
    33481 ctgacgcgct tcgccaaaac gcgcggtgtg gcgattgtca tggttgggca cgtaaccaaa
    33541 gatggctcgc tggctggccc taaagtgctg gaacactgta tcgactgttc ggtgcttctg
    33601 gatggtgatg ccgactcccg ttttcgcacc ttgcgcagcc ataaaaaccg cttcggcgcg
    33661 gtgaatgagc tgggcgtctt cgcgatgacc gaacaggggc tgcgtgaagt cagcaaccct
    33721 tcggcaattt tcttaagtcg cggagatgaa gtgacctccg gcagctccgt gatggtggtg
    33781 tgggaaggaa cgcgtccgtt gctggtggag attcaggcgc tggtcgatca ctcgatgatg
    33841 gcgaacccac gccgcgtggc agtggggctg gaacaaaacc gtctggcaat cctgctggct
    33901 gtgttgcacc gtcacggtgg tctgcaaatg gccgatcagg atgtgtttgt gaacgtggtc
    33961 ggcggcgtga aggtaaccga aaccagtgcc gacttagcgt tactgctggc gatggtttcc
    34021 agcctgcgcg acagaccgct gccgcaggat ctggtggtgt ttggtgaagt cgggctggca
    34081 ggggagatcc gcccggtgcc cagcggtcag gaacgaatct ctgaagcggc gaaacacggt
    34141 tttcgccggg cgattgttcc ggcggctaac gtgccgaaaa aagcgccgga agggatgcag
    34201 atttttggcg ttaaaaaact ctccgacgcg cttagcgtgt tcgacgactt ataatgagat
    34261 atacggaggg agatatgtcg tcatttgatt acctgaaaac tgccatcaag caacagggct
    34321 gcacgctaca gcaggtagct gatgccagcg gtatgaccaa agggtattta agccagttac
    34381 tgaatgccaa aatcaaaagc cccagcgcgc aaaagctgga ggcgttgcac cgttttttgg
    34441 ggcttgagtt tccccggcag aagaaaacga tcggtgtcgt attcggtaag ttctacccac
    34501 tgcataccgg acatatctac cttatccagc gcgcctgtag ccaggttgac gagctgcata
    34561 tcattatggg ttttgacgat acccgtgacc gcgcgttgtt cgaagacagt gccatgtcgc
    34621 agcagccgac cgtgccggat cgtctgcgtt ggttattgca aacttttaaa tatcagaaaa
    34681 atattcgcat tcatgctttc aacgaagagg gcatggagcc gtatccgcac ggctgggatg
    34741 tgtggagcaa cggcatcaaa aagtttatgg ctgaaaaagg gatccagccg gatctgatct
    34801 acacctcgga agaagccgat gcgccacagt atatggaaca tctggggatc gagacggtgc
    34861 tggtcgatcc gaaacgtacc tttatgagta tcagcggtgc gcagatccgc gaaaacccgt
    34921 tccgctactg ggaatatatt cctaccgaag tgaagccgtt ttttgtgcgt accgtggcga
    34981 tccttggcgg cgagtcgagc ggtaaatcca ccctggtaaa caaacttgcc aatatcttca
    35041 acaccaccag tgcgtgggaa tatggccgcg attatgtctt ttcacacctc ggcggtgatg
    35101 agatcgcatt gcagtattct gactacgata aaatcgcgct gggccacgct caatacattg
    35161 attttgcggt gaaatatgcc aataaagtgg catttatcga taccgatttt gtcaccactc
    35221 aggcgttctg caaaaagtac gaagggcggg aacatccgtt cgtgcaggcg ctgattgatg
    35281 aataccgttt cgatctggtg atcctgctgg agaacaacac gccgtgggtg gcggatggtt
    35341 tacgcagcct cggcagttcg gtggatcgca aagagttcca gaacttgctg gtggagatgc
    35401 tcgaagagaa caatatcgaa tttgtgcggg ttgaagagga agattacgac agtcgtttcc
    35461 tgcgctgcgt ggaactggtg cgggagatga tgggggagca gagataaccg tgatgaaact
    35521 gctcaaaggc gaggtataaa atgagttttt ttgatgagtt gaaaacctct ctggaagagg
    35581 ctgtcgagat taaacaaggt ttgaaaaaac ctgcacgggt gacccgccac gaaattgagg
    35641 atgctaaggc tgttgtagac cggaaacggt gttcacgccg catccggcat tcggtgctca
    35701 atgcctgatg cgacgcttac cgcgtcttat caggcctaca gttttacaac gtattgaaat
    35761 tgctggtttt gtaggccgga taaggcgttc gcgccgcatc cggcatttta cgcattactt
    35821 cgcaatacgc ttgtacttga tacgcttcgg ctccagcgcg tctgcgccca gcgtgcgttt
    35881 cttgtactct tcgtactcgg taaagttacc ttcgaagaac tcaactttac cttcatcctg
    35941 gtaatccaga atgtgcgtgg cgatacggtc gaggaaccaa cggtcgtgcg agataaccat
    36001 cgcacagccc gggaactcca gcagggcgtt ttccagcgcg cgcagggttt cgatatccag
    36061 gtcgttggtt ggttcgtcga gcagcagcat gttgccgcca acctgcagca gcttcgccag
    36121 atgcagacga ccgcgctcac caccggagag ttcaccaacg cgtttaccct gatcaacccc
    36181 tttaaagtta aagcggccaa cgtaggcgcg gcttggcatc tcggtgttgc cgatcttcat
    36241 gatatccagc ccgccggaaa cttcttccca aacggttttg ctgttatcca ttgagtcacg
    36301 gaactgatca accgacgcca gtttcaccgt ttcacccaaa gtgatgctgc cgctgtccgg
    36361 ctgttcctga ccagagatca tacggaacag ggtcgattta cccgcaccgt tcggaccgat
    36421 gatcccgacg atcgctcctt tcgggatcga gaagctcagg tcatcaatca gcagacgatc
    36481 gccataggat ttacgcaggt tgctgacttc cagcacttta tcgcccagac gcggtccagg
    36541 tggaataaac agttcgttgg tttcgttacg tttctgatat tcggtgctgt tcagttcttc
    36601 aaagcgcgcc agacgtgctt tacctttcga ctgacggcct ttagtacctt gacgtaccca
    36661 ttccagctct ttctcaatcg acttacgacg cgccgcttct tgtgaagctt cctgcgccag
    36721 gcgctgatct ttctgctcca gccaggagga gtagttacct tcccacggaa taccttcacc
    36781 gcggtcaagt tcgaggatcc agcctgcaac gttatcgagg aagtaacggt cgtgggtaat
    36841 cgccacaacg gtgccttcga agtcgtgcag gaagcgttcc agccaggcca cggattcggc
    36901 atccaggtgg ttggtcggtt cgtcgagcag cagcatgtct ggtttttcca gcagcaggcg
    36961 gcacaacgct acgcgacgac gttcaccacc ggagaggtta gcgattttcg cgtcccagtc
    37021 cggcagacgt agcgcatccg ccgcacgctc cagctgtacg ttcagattat gaccgtcgtg
    37081 agcctgaatg atctcttcca gacggccttg ttcagcggcc agcttgtcaa aatcggcatc
    37141 cggatcggcg tacagcgcat acacttcatc caggcgtttc agggcgttaa ccacttctga
    37201 aaccgcttct tcaatggact cacgcacggt gtgttccggg ttcagctgcg gttcctgcgg
    37261 cagataacca atcttgatgt ctggctgcgg acgcgcttca ccttcgatgt ctttatcaat
    37321 gcccgccata atgcgcagca gggtggactt acccgcgcca ttcagaccca ggacaccaat
    37381 ttttgcccca gggaagaaac tcagagagat gtttttcaaa atatgacgtt tcggcggaac
    37441 aactttgccg acacgatgca tggtataaac gaattgagcc acgttggact tcgcctctat
    37501 gtttatcgtg ataatgagtt ttcaaaggcg aagtgtagcc tttttccccg cctaatccca
    37561 gccggtcgat caccctcgca gtaaaagtaa aaaagtgtcc gtaacgtggc gtaaacggca
    37621 atgactggtt agcataaatc tattacgcgg catgacgctg cattgatgta tttacactta
    37681 gaggatgcgc ttgtggaaaa agccaaacaa gttacctggc ggctgttggc tgccggtgtc
    37741 tgtctgctga cggtcagcag cgtggcgcga gccgactcac tggatgagca gcgtagtcgt
    37801 tacgcgcaaa tcaagcaggc ctgggataat cgacaaatgg atgtggtcga acaaatgatg
    37861 cctggactga aggattatcc gctttatccc tacctggaat accgccagat caccgatgat
    37921 ctgatgaatc aaccggcggt gacggtcact aactttgttc gcgctaaccc cacgcttcct
    37981 cccgctcgca cgctgcaatc tcgtttcgtc aatgaactgg cgcggcgtga agactggcgt
    38041 ggcttgttag cctttagccc ggaaaagccc ggaactaccg aagcgcaatg taattactac
    38101 tatgcgaaat ggaacaccgg gcagagtgaa gaagcctggc aaggggcgaa agagctgtgg
    38161 ctaaccggca agagccagcc taacgcctgt gacaagttat ttagcgtctg gcgtgcgtca
    38221 ggtaaacaag atccgctggc gtatttagag cgtatccgtc tggcgatgaa agcgggtaac
    38281 acaggcctgg taacagtgct ggcagggcag atgcctgccg attaccagac tatcgcctcg
    38341 gcaatcattt cactggcgaa caaccctaat acggtactga ccttcgcgcg tacaactggc
    38401 gcgaccgatt ttacccgtca aatggcggcg gtggcgtttg ccagtgtggc gcggcaggat
    38461 gctgagaatg cacggctgat gatcccatcg cttgcccagg cgcagcagct taatgaagat
    38521 cagattcagg agctgcgcga tatcgtcgcc tggcgtttga tgggcaacga tgtcaccgac
    38581 gagcaggcga aatggcgcga tgacgccatt atgcgctcgc aatctacttc gcttattgaa
    38641 cgccgtgtac gaatggcgct tggcaccggc gatcgtcgcg gcctgaatac ctggctggcg
    38701 cgtctgccta tggaagcgaa agagaaagat gaatggcgtt actggcaggc ggatttattg
    38761 ctggaacgcg gacgtgaagc tgaagcaaaa gagattttgc atcaactcat gcaacagcgt
    38821 ggtttctacc cgatggttgc agcacaacgc atcggcgaag agtatgagct gaagattgat
    38881 aaagctccgc agaatgttga cagcgccctg actcaggggc cggagatggc gcgcgtgcgc
    38941 gagttgatgt actggaatct cgataatacc gcgcgtagcg agtgggccaa tctggtgaag
    39001 agcaagtcaa aaacagagca ggctcaactg gcgcggtatg ctttcaacaa ccaatggtgg
    39061 gatcttagcg ttcaggcaac gatcgccggg aagctgtggg atcatctgga agagcgattc
    39121 ccgctggctt acaacgatct tttcaaacgc tacaccagcg gtaaggagat cccgcaaagc
    39181 tatgcgatgg cgattgctcg tcaggagagc gcctggaatc cgaaagtgaa atcaccggta
    39241 ggggccagcg gcttgatgca gattatgcct ggtacagcga cccatacggt gaagatgttc
    39301 tctattcccg gttatagcag tcctgggcaa ttgctggatc cggaaacgaa tatcaacatt
    39361 ggcaccagtt acctgcaata tgtttatcag cagtttggca ataatcgtat tttctcctca
    39421 gcagcttata acgccggacc agggcgggtg cgaacctggc ttggcaacag cgccgggcgt
    39481 atcgacgcag tggcatttgt cgagagtatt ccattctccg agacgcgcgg ttatgtgaag
    39541 aacgtgctgg cttatgacgc ttactaccgc tatttcatgg gggataaacc gacgttgatg
    39601 agcgccacgg aatggggacg tcgttactga tccgcacgtt tatgatatgc tatcgtactc
    39661 tttagcgagt acaaccgggg gaggcatttt gcttcccccg ctaacaatgg cgacatatta
    39721 tggcccaaca atcaccctat tcagcagcga tggcagaaca gcgtcaccag gagtggttac
    39781 gttttgtcga cctgcttaag aatgcctacc aaaacgatct ccatttaccg ttgttaaacc
    39841 tgatgctgac gccagatgag cgcgaagcgt tggggactcg cgtgcgtatt gtcgaagagc
    39901 tgttgcgcgg cgaaatgagc cagcgtgagt taaaaaatga actcggcgca ggcatcgcga
    39961 cgattacgcg tggatctaac agcctgaaag ccgcgcccgt cgagctgcgc cagtggctgg
    40021 aagaggtgtt gctgaaaagc gattgatttt gtaggcctga taagacgtgg cgcatcaggc
    40081 atcgtgcacc gaatgccgga tgcggcgtga acgccttatc cgtcctacaa atacccgtaa
    40141 tttcaatatg tttggtaggc atgataagac gcggcagcgt cgcatcaggc gcttaataca
    40201 cggcattatg aaacggactc tgcgccagga tcaccgcctg gtgatagacg ctggcgcgag
    40261 tgagtttccc ggcggtaaac acgccgatcg ccccttcctt acgaccaatc tcatcaatac
    40321 cggtataacg cgacatcacg ggaccaagcg cctcaccttc acgcactttt tccagaatca
    40381 ccgcaggcaa cggcaaagta gccgaacgcg cctcgccgcg ctggctggcg ttttcaatca
    40441 ccacccaact gaaagtgctg tcaccatcga tgccagcttc aatcgccacc caaaaatcag
    40501 cctctggaag taaacggcgg gcattggcta cccgatttcg tgcgccagcg cgcgtttcct
    40561 cactgccaaa gggctgttcc ggtacaccgc tctcgacggc aacggatgca atatggcagg
    40621 atccttcgcc gaagatctcg tgaaatgcct gcagaatggc ctgaatttta gcgggattgg
    40681 tggtcgcaca gacaacttgg tgcataatca gcattactca gaaaattaac gttacagcag
    40741 tatacggaaa aaaagcatgt tacaggtata cctagtccgc cacggtgaaa cgcagtggaa
    40801 cgccgagcga cgtattcagg gccagtctga cagcccgctg accgccaaag gtgagcaaca
    40861 ggcgatgcag gtggcaaccc gtgccaaaga gcttggcatt acgcatatca tcagtagcga
    40921 tttaggacgc acccggcgta cggcggaaat catcgcccag gcctgcggct gtgacatcat
    40981 ctttgattct cgcctgcgtg aattaaacat gggtgtgctg gaaaaaagac atatcgattc
    41041 tctgaccgaa gaagaagaga actggcgtcg gcagctggtc aatggcaccg ttgacgggcg
    41101 tattcctgaa ggcgagtcaa tgcaggagct cagcgatcgc gtcaatgcgg cactggaatc
    41161 ttgccgggac ttaccgcagg gaagccgacc gctgctggtg agtcatggta ttgcactggg
    41221 atgcctggtg agtacgattc tcggattacc agcatgggca gagcgccgct tacgtctgcg
    41281 taactgttct atttcgcgcg tggattatca ggaaagcctg tggctggcgt ctggctgggt
    41341 cgttgaaacc gcaggggaca tctcgcatct ggacgcccct gcattagatg agctgcagcg
    41401 ttaacgacgg atcggaatca gcagttcaca gcgtagatta attgggcgat ctcccgcttt
    41461 ggcatcttct gccgggtagt atcgctcaat atcctgacct ttacggcgcg tcaggttgag
    41521 cattggcatg cacgttccgt ataccgtcag gataaactcc tgcacgccgg ttcccagacc
    41581 ttcataggta aacatcacat attcgccgcc ctgcagcatc accggatgcc ccgtcagtac
    41641 atagccatct gcctgatcct gggctaacgc ggtggtatag aatacctctt gctcgtcgtc
    41701 tttatcctga ctcggacgcg tttcattcag gccgtagagc accggcggaa tggtcggcgc
    41761 gttgccgaga aaatcgtgcc agaactgata acgcatttca tggcggaaat cagagatttg
    41821 ctccagcgaa caggagtagc tctgggtaac accaatcagc ggcgtatctt ccagggtgac
    41881 aaatttgtgc tctggcatag tgaattcacc caggcgtagc ggcgggcgaa taccaaaggc
    41941 gctccattca ggagaacggc ggtaaagtgc aggagtctgg gcaaactgct tcttgaatgc
    42001 gcgggtaaat gtctgttgag agtcgaagcg gtattgcagc gcgatgtcca gaatcggacg
    42061 cgcagtcagg cgtagtgcga ccgccgattt cgacaaacga cgagcacgaa tatacgcgcc
    42121 aatagcatgg ccagtgacat ctttaaacat tctctgtaag tgccacttgg aataacctgc
    42181 tttcgccgct acattgtcga gcgacagggg ctgatccaga tgaccttcca gccagattaa
    42241 aaggtcgcga ataatgccgg cctgatccat aaaatatcct catcctttca acaacgagca
    42301 cctgacatca ggtaattgga taatagcatt ttttgctgtt ttagcattca gtgttttttt
    42361 cttagtagag tatgttttag ggcttgacgg aaataaaagt attgagattt tgttcttaat
    42421 caatatgtta tttaccgtga cgaactaatt gctcgtgtaa tagataaaaa tggtaacaat
    42481 atgaaataca agcatttgat cctgtcttta agcctgataa tgctggggcc attggctcat
    42541 gcagaagaga ttggttcggt cgacaccgta tttaaaatga tcggcccgga tcacaaaatt
    42601 gttgtggaag cctttgatga tcccgatgtg aaaaatgtca cctgttatgt gagccgggcg
    42661 aaaaccggtg gtattaaagg gggattgggt ctggcggaag atacctccga tgcggccatt
    42721 tcttgtcagc aagtcgggcc gattgaactg tcggatcgta ttaaaaacgg caaagctcag
    42781 ggcgaggtag tattcaaaaa acgcacgtcc ctagtcttta agtcgttaca ggtcgtgcgc
    42841 ttttatgatg ccaaacgcaa cgcgctcgct tatctggctt actccgacaa agttgtagaa
    42901 ggttcgccga aaaacgcgat tagcgcggtt cctgtcatgc cgtggcggca ataacagagg
    42961 cgatttatgc aacgggaaac ggtctggtta gtggaagatg agcaagggat agccgacacg
    43021 ctggtctaca tgttgcagca ggaaggtttt gccgtcgagg tctttgagcg aggcttgccg
    43081 gtgctggata aagctcgcaa gcaggtaccc gacgtcatga ttctcgatgt tggtctgccg
    43141 gatattagcg gctttgaatt gtgccgccag ttactggcgc tccatccggc gttacctgta
    43201 ctgttcctga cggcccgaag tgaagaggtc gatcgcctgc ttgggctgga aattggtgct
    43261 gacgactacg tggctaaacc gttttcaccc cgcgaagtgt gcgccagggt gcgcacctta
    43321 ctgcgtcggg tgaagaagtt ctcgacgccg tctcccgtca tccgtattgg acattttgaa
    43381 ttgaatgaac ccgcggcgca gatcagctgg tttgacacgc cattagcgct gactcggtat
    43441 gagtttttat tgttgaagac gttactcaag tcaccgggcc gcgtctggtc ccgccagcaa
    43501 ctgatggata gcgtatggga agatgcgcag gacacctacg atcgcaccgt cgatacccac
    43561 attaaaacgc tgcgtgccaa gctgcgcgcc atcaaccccg atctttcacc gattaatact
    43621 catcgcggca tgggatatag cctgaggggc ctgtaatgcg tatcggcatg cggttgttgc
    43681 tgggctattt tttactggtg gcggtggcag cctggttcgt actggccatt tttgtcaaag
    43741 aagttaaacc gggcgtgcga agagcaacgg aggggacgtt gatcgacacc gcaacgttgc
    43801 tggcggagct ggcgcgtccc gatttgctct ctggggaccc aacgcatggg caactggcgc
    43861 aggcgtttaa tcagctacaa catcgcccgt ttcgcgccaa tatcggtggc attaacaaag
    43921 tgcgcaatga atatcatgtc tatatgaccg atgcgcaggg caaagtattg ttcgattcgg
    43981 caaataaagc cgttggacag gattattcgc gctggaatga cgtctggcta acgttgcgtg
    44041 gtcagtatgg tgcgcgcagc acgttgcaaa atcctgccga tcccgaaagt tctgtgatgt
    44101 atgttgccgc accgattatg gacggctcgc ggcttattgg cgttttgagc gtaggcaaac
    44161 cgaacgcggc gatggctccg gtcattaagc gtagcgagcg gcgaatttta tgggccagcg
    44221 ccattttgtt ggggattgca ctggtgattg gcgcaggcat ggtttggtgg atcaaccgct
    44281 ctattgcccg gctcactcgc tatgctgatt ccgtcactga caataagccc gttcctctcc
    44341 ccgatctcgg tagtagcgag ttgcgtaaac tcgcgcaggc gctggaaagt atgcgcgtga
    44401 agctggaagg gaaaaactat attgagcagt atgtttacgc attaactcat gagctaaaaa
    44461 gcccactggc ggcgattcgt ggagcggcgg aaattttacg cgaaggtccg ccgccggaag
    44521 tggtggctcg ttttactgac aacattctga cgcaaaatgc gcgtatgcag gcattggtag
    44581 aaacgttact acgccaggca agactggaga atcgtcagga agtcgttctg actgctgttg
    44641 atgtggcggc attattccgc cgcgtcagcg aagcgcgcac cgtgcagttg gcagaaaaaa
    44701 aaatcactct gcatgttacg cccaccgagg ttaacgttgc tgctgaaccg gcgttactgg
    44761 agcaggcgct gggaaattta ctggataacg ccatcgattt tacccctgag agcggttgca
    44821 tcacgctaag cgccgaagtg gatcaggaac acgtcacgct taaggtgctg gataccggta
    44881 gtggtattcc tgactacgcg ctttcacgta tttttgaacg cttttactct ttgcctcgtg
    44941 caaatgggca aaaaagcagc ggtctggggt tggcgttcgt cagtgaggtc gcccgtttgt
    45001 ttaacggcga agtcacgctg cgcaacgtgc aggaaggtgg cgtgctggcc tcgcttcgac
    45061 ttcaccgtca cttcacatag cttcaaattc ttcccacata gtcttcgtat cctgctgcca
    45121 ttgcaaagga gaagactatg ttgaaatccc ccctgttctg gaaaatgact agcctgtttg
    45181 gtgcagtatt gctgttgttg attccgataa tgctgattcg gcaggtgatt gtcgaacgtg
    45241 ctgattaccg tagcgatgtg gaagatgcga ttcgccaaag taccagcggg ccgcaaaaac
    45301 tcgttgggcc gctcatcgct attcctgtga ccgagcttta tacggtgcag gaagaggata
    45361 aaaccgtgga gcggaaacga agttttatcc atttttggtt acctgagtca ttgatggttg
    45421 atggcaatca gaacgtggaa gaacgcaaga tagggattta taccggtcag gtctggcaca
    45481 gtgatttaac gttaaaagcc gatttcgatg tttcgcgtct tagcgaactc aacgcgccaa
    45541 atatcacctt aggcaagcca tttattgtga ttagcgtcgg ggatgcgcgt ggtattggtg
    45601 tggtgaaagc gcctgaagtt aacggaacgg cgctgaccat tgaacccggc accgggttag
    45661 agcaaggcgg gcagggcgtg catatccctt tacctgaagg ggactggcgg aagcagaacc
    45721 tgaagctgaa tatggccctg aatttaagcg gtaccggcga tctttctgtg gtgcctggcg
    45781 ggcgtaatag cgaaatgacc ttaaccagca actggccgca tcccagtttt ttaggtgatt
    45841 ttctaccagc caaacgggaa gttagcgagt caggttttca ggcgcactgg caaagcagct
    45901 ggtttgctaa taatctcggt gagcgttttg cttcaggcaa tgataccggc tgggaaaact
    45961 tcccggcgtt tagcgtcgca gtaacgacgc cagccgatca ataccaatta actgaccggg
    46021 cgactaagta cgccattctg ctgattgcac tgacttttat ggcgttcttt gtttttgaaa
    46081 cgctcaccgc gcaacgttta cacccaatgc aatatttgct ggtggggctt tcattggtga
    46141 tgttttattt gctcttgctg gcgctttctg aacataccgg ttttaccgtg gcatggataa
    46201 tcgccagtct gattggggcg ataatgaacg gtatttattt gcaagcggta ttgaaaggtt
    46261 ggtgcaacag catgttgttt accctcgcgc tgttgttgct ggatggtgtg atgtggggac
    46321 tgctcaactc tgccgatagc gcgctgttgt tgggaaccag tgtgctggtg gtggcgctgg
    46381 ccggcatgat gtttgtgacc cgtaatatcg actggtatgc gttttcactg ccgaaaatga
    46441 aagccagtaa agaagttaca acggacgatg agttacgtat ctggaaataa ggttgaaaaa
    46501 taaaaacggc gctaaaaagc gccgtttttt ttgacggtgg taaagccgat taatcttcca
    46561 gatcaccgca gaagcgataa ccttcaccgt gaatggtggc gatgatttcc ggcgtatccg
    46621 gcgtagattc gaaatgttta cgaatacggc ggatcgtcac gtctacagta cggtcgtgcg
    46681 gtttcagctc acggccggtc attttcttca gcagttcagc acgggactga attttgcctg
    46741 ggttttcaca gaagtgaagc atggcgcgga actcgctgcg cggcagcttg tactgctcgc
    46801 catcagggcc gatcaacgaa cggctgttga tgtccagttc ccaaccattg aacttgtagc
    46861 tttcaacgct acgacgttct tcgctgacag tacccagatt catggtacgg gacagtaggt
    46921 tgcgtgcacg aatcgtcagt tcacgcgggt tgaacggttt ggtgatgtag tcatctgcac
    46981 cgatttcgag gccgagaatt ttatcgactt cgttgtcacg gccagtcagg aacatcaacg
    47041 caacattcgc ctgctcgcgc agttcacgcg ctaacagaag accgttctta cccggcagat
    47101 tgatatccat gatcaccagg ttgatgtcat attcagagag gatctgatgc atttccgcgc
    47161 catctgtcgc ttcgaaaaca tcatagcctt ccgcttcgaa aatacttttc aacgtgttgc
    47221 gtgttaccaa ctcgtcttca acgataagaa tgtgcggggt ctgcatgttt gctacctaaa
    47281 ttgccaacta aatcgaaaca ggaagtacaa aagtccctga cctgcctgat gcatgctgca
    47341 aattaacatg atcggcgtaa catgactaaa gtacgtaatt gcgttcttga tgcactttcc
    47401 atcaacgtca acaacatcat tagcttggtc gtgggtactt tccctcagga cccgacagtg
    47461 tcaaaaacgg ctgtcatcct aaccatttta acagcaacat aacaggctaa gaggggccgg
    47521 acacccaata aaactacgct tcgttgacat atatcaagtt caattgtagc acgttaacag
    47581 tttgatgaaa tcatcgtatc taaatgctag ctttcgtcac attattttaa taatccaact
    47641 agttgcatca tacaactaat aaacgtggtg aatccaattg tcgagattta ttttttataa
    47701 aattatccta agtaaacaga aggatatgta gcatttttta acaactcaac cgttagtaca
    47761 gtcaggaaat agtttagcct tttttaagct aagtaaaggg ctttttctgc gacttacgtt
    47821 aagaatttgt aaattcgcac cgcgtaataa gttgacagtg atcacccggt tcgcggttat
    47881 ttgatcaaga agagtggcaa tatgcgtata acgattattc tggtcgcacc cgccagagca
    47941 gaaaatattg gggcagcggc gcgggcaatg aaaacgatgg ggtttagcga tctgcggatt
    48001 gtcgatagtc aggcacacct ggagccagcc acccgctggg tcgcacatgg atctggtgat
    48061 attattgata atattaaagt tttcccgaca ttggctgaat cgttacacga tgtcgatttc
    48121 actgtcgcca ccactgcgcg cagtcgggcg aaatatcatt actacgccac gccagttgaa
    48181 ctggtgccgc tgttagagga aaaatcttca tggatgagcc atgccgcgct ggtgtttggt
    48241 cgcgaagatt ccgggttgac taacgaagag ttagcgttgg ctgacgttct tactggtgtg
    48301 ccgatggtgg cggattatcc ttcgctcaat ctggggcagg cggtgatggt ctattgctat
    48361 caattagcaa cattaataca acaaccggcg aaaagtgatg caacggcaga ccaacatcaa
    48421 ctgcaagctt tacgcgaacg agccatgaca ttgctgacga ctctggcagt ggcagatgac
    48481 ataaaactgg tcgactggtt acaacaacgc ctggggcttt tagagcaacg agacacggca
    48541 atgttgcacc gtttgctgca tgatattgaa aaaaatatca ccaaataaaa aacgccttag
    48601 taagtatttt tcagcttttc attctgactg caacgggcaa tatgtctctg tgtggattaa
    48661 aaaaagagtg tctgatagca gcttctgaac tggttacctg ccgtgagtaa attaaaattt
    48721 tattgactta ggtcactaaa tactttaacc aatataggca tagcgcacag acagataaaa
    48781 attacagagt acacaacatc catgaaacgc attagcacca ccattaccac caccatcacc
    48841 attaccacag gtaacggtgc gggctgacgc gtacaggaaa cacagaaaaa agcccgcacc
    48901 tgacagtgcg ggcttttttt ttcgaccaaa ggtaacgagg taacaaccat gcgagtgttg
    48961 aagttcggcg gtacatcagt ggcaaatgca gaacgttttc tgcgtgttgc cgatattctg
    49021 gaaagcaatg ccaggcaggg gcaggtggcc accgtcctct ctgcccccgc caaaatcacc
    49081 aaccacctgg tggcgatgat tgaaaaaacc attagcggcc aggatgcttt acccaatatc
    49141 agcgatgccg aacgtatttt tgccgaactt ttgacgggac tcgccgccgc ccagccgggg
    49201 ttcccgctgg cgcaattgaa aactttcgtc gatcaggaat ttgcccaaat aaaacatgtc
    49261 ctgcatggca ttagtttgtt ggggcagtgc ccggatagca tcaacgctgc gctgatttgc
    49321 cgtggcgaga aaatgtcgat cgccattatg gccggcgtat tagaagcgcg cggtcacaac
    49381 gttactgtta tcgatccggt cgaaaaactg ctggcagtgg ggcattacct cgaatctacc
    49441 gtcgatattg ctgagtccac ccgccgtatt gcggcaagcc gcattccggc tgatcacatg
    49501 gtgctgatgg caggtttcac cgccggtaat gaaaaaggcg aactggtggt gcttggacgc
    49561 aacggttccg actactctgc tgcggtgctg gctgcctgtt tacgcgccga ttgttgcgag
    49621 atttggacgg acgttgacgg ggtctatacc tgcgacccgc gtcaggtgcc cgatgcgagg
    49681 ttgttgaagt cgatgtccta ccaggaagcg atggagcttt cctacttcgg cgctaaagtt
    49741 cttcaccccc gcaccattac ccccatcgcc cagttccaga tcccttgcct gattaaaaat
    49801 accggaaatc ctcaagcacc aggtacgctc attggtgcca gccgtgatga agacgaatta
    49861 ccggtcaagg gcatttccaa tctgaataac atggcaatgt tcagcgtttc tggtccgggg
    49921 atgaaaggga tggtcggcat ggcggcgcgc gtctttgcag cgatgtcacg cgcccgtatt
    49981 tccgtggtgc tgattacgca atcatcttcc gaatacagca tcagtttctg cgttccacaa
    50041 agcgactgtg tgcgagctga acgggcaatg caggaagagt tctacctgga actgaaagaa
    50101 ggcttactgg agccgctggc agtgacggaa cggctggcca ttatctcggt ggtaggtgat
    50161 ggtatgcgca ccttgcgtgg gatctcggcg aaattctttg ccgcactggc ccgcgccaat
    50221 atcaacattg tcgccattgc tcagggatct tctgaacgct caatctctgt cgtggtaaat
    50281 aacgatgatg cgaccactgg cgtgcgcgtt actcatcaga tgctgttcaa taccgatcag
    50341 gttatcgaag tgtttgtgat tggcgtcggt ggcgttggcg gtgcgctgct ggagcaactg
    50401 aagcgtcagc aaagctggct gaagaataaa catatcgact tacgtgtctg cggtgttgcc
    50461 aactcgaagg ctctgctcac caatgtacat ggccttaatc tggaaaactg gcaggaagaa
    50521 ctggcgcaag ccaaagagcc gtttaatctc gggcgcttaa ttcgcctcgt gaaagaatat
    50581 catctgctga acccggtcat tgttgactgc acttccagcc aggcagtggc ggatcaatat
    50641 gccgacttcc tgcgcgaagg tttccacgtt gtcacgccga acaaaaaggc caacacctcg
    50701 tcgatggatt actaccatca gttgcgttat gcggcggaaa aatcgcggcg taaattcctc
    50761 tatgacacca acgttggggc tggattaccg gttattgaga acctgcaaaa tctgctcaat
    50821 gcaggtgatg aattgatgaa gttctccggc attctttctg gttcgctttc ttatatcttc
    50881 ggcaagttag acgaaggcat gagtttctcc gaggcgacca cgctggcgcg ggaaatgggt
    50941 tataccgaac cggacccgcg agatgatctt tctggtatgg atgtggcgcg taaactattg
    51001 attctcgctc gtgaaacggg acgtgaactg gagctggcgg atattgaaat tgaacctgtg
    51061 ctgcccgcag agtttaacgc cgagggtgat gttgccgctt ttatggcgaa tctgtcacaa
    51121 ctcgacgatc tctttgccgc gcgcgtggcg aaggcccgtg atgaaggaaa agttttgcgc
    51181 tatgttggca atattgatga agatggcgtc tgccgcgtga agattgccga agtggatggt
    51241 aatgatccgc tgttcaaagt gaaaaatggc gaaaacgccc tggccttcta tagccactat
    51301 tatcagccgc tgccgttggt actgcgcgga tatggtgcgg gcaatgacgt tacagctgcc
    51361 ggtgtctttg ctgatctgct acgtaccctc tcatggaagt taggagtctg acatggttaa
    51421 agtttatgcc ccggcttcca gtgccaatat gagcgtcggg tttgatgtgc tcggggcggc
    51481 ggtgacacct gttgatggtg cattgctcgg agatgtagtc acggttgagg cggcagagac
    51541 attcagtctc aacaacctcg gacgctttgc cgataagctg ccgtcagaac cacgggaaaa
    51601 tatcgtttat cagtgctggg agcgtttttg ccaggaactg ggtaagcaaa ttccagtggc
    51661 gatgaccctg gaaaagaata tgccgatcgg ttcgggctta ggctccagtg cctgttcggt
    51721 ggtcgcggcg ctgatggcga tgaatgaaca ctgcggcaag ccgcttaatg acactcgttt
    51781 gctggctttg atgggcgagc tggaaggccg tatctccggc agcattcatt acgacaacgt
    51841 ggcaccgtgt tttctcggtg gtatgcagtt gatgatcgaa gaaaacgaca tcatcagcca
    51901 gcaagtgcca gggtttgatg agtggctgtg ggtgctggcg tatccgggga ttaaagtctc
    51961 gacggcagaa gccagggcta ttttaccggc gcagtatcgc cgccaggatt gcattgcgca
    52021 cgggcgacat ctggcaggct tcattcacgc ctgctattcc cgtcagcctg agcttgccgc
    52081 gaagctgatg aaagatgtta tcgctgaacc ctaccgtgaa cggttactgc caggcttccg
    52141 gcaggcgcgg caggcggtcg cggaaatcgg cgcggtagcg agcggtatct ccggctccgg
    52201 cccgaccttg ttcgctctgt gtgacaagcc ggaaaccgcc cagcgcgttg ccgactggtt
    52261 gggtaagaac tacctgcaaa atcaggaagg ttttgttcat atttgccggc tggatacggc
    52321 gggcgcacga gtactggaaa actaaatgaa actctacaat ctgaaagatc acaacgagca
    52381 ggtcagcttt gcgcaagccg taacccaggg gttgggcaaa aatcaggggc tgttttttcc
    52441 gcacgacctg ccggaattca gcctgactga aattgatgag atgctgaagc tggattttgt
    52501 cacccgcagt gcgaagatcc tctcggcgtt tattggtgat gaaatcccac aggaaatcct
    52561 ggaagagcgc gtgcgcgcgg cgtttgcctt cccggctccg gtcgccaatg ttgaaagcga
    52621 tgtcggttgt ctggaattgt tccacgggcc aacgctggca tttaaagatt tcggcggtcg
    52681 ctttatggca caaatgctga cccatattgc gggtgataag ccagtgacca ttctgaccgc
    52741 gacctccggt gataccggag cggcagtggc tcatgctttc tacggtttac cgaatgtgaa
    52801 agtggttatc ctctatccac gaggcaaaat cagtccactg caagaaaaac tgttctgtac
    52861 attgggcggc aatatcgaaa ctgttgccat cgacggcgat ttcgatgcct gtcaggcgct
    52921 ggtgaagcag gcgtttgatg atgaagaact gaaagtggcg ctagggttaa actcggctaa
    52981 ctcgattaac atcagccgtt tgctggcgca gatttgctac tactttgaag ctgttgcgca
    53041 gctgccgcag gagacgcgca accagctggt tgtctcggtg ccaagcggaa acttcggcga
    53101 tttgacggcg ggtctgctgg cgaagtcact cggtctgccg gtgaaacgtt ttattgctgc
    53161 gaccaacgtg aacgataccg tgccacgttt cctgcacgac ggtcagtggt cacccaaagc
    53221 gactcaggcg acgttatcca acgcgatgga cgtgagtcag ccgaacaact ggccgcgtgt
    53281 ggaagagttg ttccgccgca aaatctggca actgaaagag ctgggttatg cagccgtgga
    53341 tgatgaaacc acgcaacaga caatgcgtga gttaaaagaa ctgggctaca cttcggagcc
    53401 gcacgctgcc gtagcttatc gtgcgctgcg tgatcagttg aatccaggcg aatatggctt
    53461 gttcctcggc accgcgcatc cggcgaaatt taaagagagc gtggaagcga ttctcggtga
    53521 aacgttggat ctgccaaaag agctggcaga acgtgctgat ttacccttgc tttcacataa
    53581 tctgcccgcc gattttgctg cgttgcgtaa attgatgatg aatcatcagt aaaatctatt
    53641 cattatctca atcaggccgg gtttgctttt atgcagcccg gcttttttat gaagaaatta
    53701 tggagaaaaa tgacagggaa aaaggagaaa ttctcaataa atgcggtaac ttagagatta
    53761 ggattgcgga gaataacaac cgccgttctc atcgagtaat ctccggatat cgacccataa
    53821 cgggcaatga taaaaggagt aacctgtgaa aaagatgcaa tctatcgtac tcgcactttc
    53881 cctggttctg gtcgctccca tggcagcaca ggctgcggaa attacgttag tcccgtcagt
    53941 aaaattacag ataggcgatc gtgataatcg tggctattac tgggatggag gtcactggcg
    54001 cgaccacggc tggtggaaac aacattatga atggcgaggc aatcgctggc acctacacgg
    54061 accgccgcca ccgccgcgcc accataagaa agctcctcat gatcatcacg gcggtcatgg
    54121 tccaggcaaa catcaccgct aaatgacaaa tgccgggtaa caatccggca ttcagcgcct
    54181 gatgcgacgc tggcgcgtct tatcaggcct acgttaattc tgcaatatat tgaatctgca
    54241 tgcttttgta ggcaggataa ggcgttcacg ccgcatccgg cattgactgc aaacttaacg
    54301 ctgctcgtag cgtttaaaca ccagttcgcc attgctggag gaatcttcat caaagaagta
    54361 accttcgcta ttaaaaccag tcagttgctc tggtttggtc agccgatttt caataatgaa
    54421 acgactcatc agaccgcgtg ctttcttagc gtagaagctg atgatcttaa atttgccgtt
    54481 cttctcatcg aggaacaccg gcttgataat ctcggcattc aatttcttcg gcttcaccga
    54541 tttaaaatac tcatctgacg ccagattaat caccacatta tcgccttgtg ctgcgagcgc
    54601 ctcgttcagc ttgttggtga tgatatctcc ccagaattga tacagatctt tccctcgggc
    54661 attctcaaga cggatcccca tttccagacg ataaggctgc attaaatcga gcgggcggag
    54721 tacgccatac aagccggaaa gcattcgcaa atgctgttgg gcaaaatcga aatcgtcttc
    54781 gctgaaggtt tcggcctgca agccggtgta gacatcacct ttaaacgcca gaatcgcctg
    54841 gcgggcattc gccggcgtga aatctggctg ccagtcatga aagcgagcgg cgttgatacc
    54901 cgccagtttg tcgctgatgc gcatcagcgt gctaatctgc ggaggcgtca gtttccgcgc
    54961 ctcatggatc aactgctggg aattgtctaa cagctccggc agcgtatagc gcgtggtggt
    55021 caacgggctt tggtaatcaa gcgttttcgc aggtgaaata agaatcagca tatccagtcc
    55081 ttgcaggaaa tttatgccga ctttagcaaa aaatgagaat gagttgatcg atagttgtga
    55141 ttactcctgc gaaacatcat cccacgcgtc cggagaaagc tggcgaccga tatccggata
    55201 acgcaatgga tcaaacaccg ggcgcacgcc gagtttacgc tggcgtagat aatcactggc
    55261 aatggtatga accacaggcg agagcagtaa aatggcggtc aaattggtaa tagccatgca
    55321 ggccattatg atatctgcca gttgccacat cagcggaagg cttagcaagg tgccgccgat
    55381 gaccgttgcg aaggtgcaga tccgcaaaca ccagatcgct ttagggttgt tcaggcgtaa
    55441 aaagaagaga ttgttttcgg cataaatgta gttggcaacg atggagctga aggcaaacag
    55501 aataaccaca agggtaacaa actcagcacc ccaggaaccc attagcaccc gcatcgcctt
    55561 ctggataagc tgaatacctt ccagcggcat gtaggttgtg ccgttacccg ccagtaatat
    55621 cagcatggcg cttgccgtac agatgaccag ggtgtcgata aaaatgccaa tcatctggac
    55681 aatcccttgc gctgccggat gcggaggcca ggacgccgct gccgctgccg cgtttggcgt
    55741 cgaacccatt cccgcctcat tggaaaacat actgcgctga aaaccgttag taatcgcctg
    55801 gcttaaggta tatcccgccg cgccgcctgc cgcttcctgc cagccaaaag cactctcaaa
    55861 aatagaccaa atgacgtggg gaagttgccc gatattcatt acgcaaatta ccaggctggt
    55921 cagtacccag attatcgcca tcaacgggac aaacccctgc atgagccggg cgacgccatg
    55981 aagaccgcga gtgattgcca gcagagtaaa gacagcgaga ataatgcctg tcaccagcgg
    56041 gggaaaatca aaagaaaaac tcagggcgcg ggcaacggcg ttcgcttgaa ctccgctgaa
    56101 aattatgcca taggcgatga gcaaaaagac ggcgaacaga acgcccatcc agcgcatccc
    56161 cagcccgcgc gccatatacc atgccggtcc gccacgaaac tgcccattga cgtcacgttc
    56221 tttataaagt tgtgccagag aacattcggc aaacgaggtc gccatgccga taaacgcggc
    56281 aacccacatc caaaagacgg ctccaggtcc accggcggta atagccagcg caacgccggc
    56341 caggttgccg ctacccacgc gcgccgcaag actggtacac aatgactgaa atgaggttaa
    56401 accgcctggc tgtggatgaa tgctattttt aagacttttg ccaaactggc ggatgtagcg
    56461 aaactgcaca aatccggtgc gaaaagtgaa ccaacaacct gcgccgaaga gcaggtaaat
    56521 cattaccgat ccccaaagga cgctgttaat gaaggagaaa aaatctggca tgcatatccc
    56581 tcttattgcc ggtcgcgatg actttcctgt gtaaacgtta ccaattgttt aagaagtata
    56641 tacgctacga ggtacttgat aacttctgcg tagcatacat gaggttttgt ataaaaatgg
    56701 cgggcgatat caacgcagtg tcagaaatcc gaaacagtct cgcctggcga taaccgtctt
    56761 gtcggcggtt gcgctgacgt tgcgtcgtga tatcatcagg gcagaccggt tacatccccc
    56821 taacaagctg tttaaagaga aatactatca tgacggacaa attgacctcc cttcgtcagt
    56881 acaccaccgt agtggccgac actggggaca tcgcggcaat gaagctgtat caaccgcagg
    56941 atgccacaac caacccttct ctcattctta acgcagcgca gattccggaa taccgtaagt
    57001 tgattgatga tgctgtcgcc tgggcgaaac agcagagcaa cgatcgcgcg cagcagatcg
    57061 tggacgcgac cgacaaactg gcagtaaata ttggtctgga aatcctgaaa ctggttccgg
    57121 gccgtatctc aactgaagtt gatgcgcgtc tttcctatga caccgaagcg tcaattgcga
    57181 aagcaaaacg cctgatcaaa ctctacaacg atgctggtat tagcaacgat cgtattctga
    57241 tcaaactggc ttctacctgg cagggtatcc gtgctgcaga acagctggaa aaagaaggca
    57301 tcaactgtaa cctgaccctg ctgttctcct tcgctcaggc tcgtgcttgt gcggaagcgg
    57361 gcgtgttcct gatctcgccg tttgttggcc gtattcttga ctggtacaaa gcgaataccg
    57421 ataagaaaga gtacgctccg gcagaagatc cgggcgtggt ttctgtatct gaaatctacc
    57481 agtactacaa agagcacggt tatgaaaccg tggttatggg cgcaagcttc cgtaacatcg
    57541 gcgaaattct ggaactggca ggctgcgacc gtctgaccat cgcaccggca ctgctgaaag
    57601 agctggcgga gagcgaaggg gctatcgaac gtaaactgtc ttacaccggc gaagtgaaag
    57661 cgcgtccggc gcgtatcact gagtccgagt tcctgtggca gcacaaccag gatccaatgg
    57721 cagtagataa actggcggaa ggtatccgta agtttgctat tgaccaggaa aaactggaaa
    57781 aaatgatcgg cgatctgctg taatcattct tagcgtgacc gggaagtcgg tcacgctacc
    57841 tcttctgaag cctgtctgtc actcccttcg cagtgtatca ttctgtttaa cgagactgtt
    57901 taaacggaaa aatcttgatg aatactttac gtattggctt agtttccatc tctgatcgcg
    57961 catccagcgg cgtttatcag gataaaggca tccctgcgct ggaagaatgg ctgacatcgg
    58021 cgctaaccac gccgtttgaa ctggaaaccc gcttaatccc cgatgagcag gcgatcatcg
    58081 agcaaacgtt gtgtgagctg gtggatgaaa tgagttgcca tctggtgctc accacgggcg
    58141 gaactggccc ggcgcgtcgt gacgtaacgc ccgatgcgac gctggcagta gcggaccgcg
    58201 agatgcctgg ctttggtgaa cagatgcgcc agatcagcct gcattttgta ccaactgcga
    58261 tcctttcgcg tcaggtgggc gtgattcgca aacaggcgct gatccttaac ttacccggtc
    58321 agccgaagtc tattaaagag acgctggaag gtgtgaagga cgctgagggt aacgttgtgg
    58381 tacacggtat ttttgccagc gtaccgtact gcattcagtt gctggaaggg ccatacgttg
    58441 aaacggcacc ggaagtggtt gcagcattca gaccgaagag tgcaagacgc gacgttagcg
    58501 aataaaaaaa tccccccgag cggggggatc tcaaaacaat tagtgggatt caccaatcgg
    58561 cagaacggtg cgaccaaact gctcgttcag tacttcaccc atcgccagat agattgcgct
    58621 ggcaccgcag atcagcccaa tccagccggc aaagtggatg attgcggcgt taccggcaat
    58681 gttaccgatc gccagcaggg caaacagcac ggtcaggcta aagaaaacga attgcagaac
    58741 gcgtgcgcct ttcagcgtgc cgaagaacat aaacagcgta aatacgcccc acagacccag
    58801 gtagacacca aggaactgtg catttggcgc atcggtcaga cccagtttcg gcatcagcag
    58861 aatcgcaacc agcgtcagcc agaaagaacc gtaagaggtg aatgcggtta aaccgaaagt
    58921 gttgcctttt ttgtactcca gcagaccagc aaaaatttgc gcgatgccgc cgtagaaaat
    58981 gcccatggca agaataatac cgtccagagc gaaataaccc acgttgtgca ggttaagcag
    59041 aatggtggtc atgccgaagc ccatcaggcc cagcggtgcc ggattagcca acttagtgtt
    59101 gcccataatt cctcaaaaat catcatcgaa tgaatggtga aataatttcc ctgaataact
    59161 gtagtgtttt cagggcgcgg cataataatc agccagtggg gcagtgtcta cgatcttttg
    59221 aggggaaaat gaaaattttc cccggtttcc ggtatcagac ctgagtggcg ctaaccatcc
    59281 ggcgcaggca ggcgatttgc agtacggctg gaatcgtcac gcgataggcg ctgccgctga
    59341 ccgctttaac cccatttagt gccgcaccta cagggcctcc cagccccgcg ccgcgcagca
    59401 aaccatgccc aagtacgctc attgctgcgt gggtgcgtaa aatgcgggtc agttggctgg
    59461 aaagcaaatg cgacacacct tttgccaata atttgtcttt catcagcagc ggcagcagct
    59521 cttccagctc attcaccctg gcatcgaccg cgtgcagaaa ctcctgctta tgttcctcgt
    59581 ccattttctt ccaggtatta cgcagaaatt gttccagtaa ctgttgctca atttcaaacg
    59641 tagacatctc tttgtcggct ttcagcttca atcgctttga aacatcgagc aaaatggccc
    59701 gatacaattt accgtgtccg cgcagtttgt tggcgatact atcgccacca aaatgctgta
    59761 attctccggc aatcagctgc cagttgcggc gatgttgctc gggatgccct tccatcgatt
    59821 taaacagttc gttgcgcatc agtacgctgg agaggcgagt tttgcctttt tcattatggg
    59881 tgagcaatcg ggcgaaattt gccaactgtt cctcactaca atgctgaaga aaatccagat
    59941 ctgaatcatt caggtaatta acattcattt tttgtggctt ctatattctg gcgttagtcg
    60001 tcgccgataa ttttcagcgt ggccatatcc gatgagttca ccgtatgacc cgaaaaggtg
    60061 atttttgaga cgcagcgttt attgtcgtta tcgctgttaa tgttgatcca gtcagtggtt
    60121 tgcccttctt ttatttctga aggaatattc aggctctgac tggcgctacg ggcggctttg
    60181 aaataaaccg atgcaccgct taactgtaaa tcgccatggt cggcagagag ttgtatgcgt
    60241 ttcacaatgc gacaaacagg aagtttcagc gccagatcgt tggtttcgtt acgcggcatt
    60301 gcaatggcgc cgaggagttt atggtcgttt gcctgcgccg tgcagcacag catcaggcta
    60361 atcgccaggc tggcggaaat cgtaaaaacg gatttcataa ggattctctt agtgggaaga
    60421 ggtaggggga tgaataccca ctagtttact gctgataaag agaagattca ggcacgtaat
    60481 cttttctttt tattacaatt ttttgatgaa tgccttggct gcgattcatt ctttatatga
    60541 ataaaattgc tgtcaatttt acgtcttgtc ctgccatatc gcgaaatttc tgcgcaaaag
    60601 cacaaaaaat ttttgcatct cccccttgat gacgtggttt acgaccccat ttagtagtca
    60661 accgcagtga gtgagtctgc aaaaaaatga aattgggcag ttgaaaccag acgtttcgcc
    60721 cctattacag actcacaacc acatgatgac cgaatatata gtggagacgt ttagatgggt
    60781 aaaataattg gtatcgacct gggtactacc aactcttgtg tagcgattat ggatggcacc
    60841 actcctcgcg tgctggagaa cgccgaaggc gatcgcacca cgccttctat cattgcctat
    60901 acccaggatg gtgaaactct agttggtcag ccggctaaac gtcaggcagt gacgaacccg
    60961 caaaacactc tgtttgcgat taaacgcctg attggtcgcc gcttccagga cgaagaagta
    61021 cagcgtgatg tttccatcat gccgttcaaa attattgctg ctgataacgg cgacgcatgg
    61081 gtcgaagtta aaggccagaa aatggcaccg ccgcagattt ctgctgaagt gctgaaaaaa
    61141 atgaagaaaa ccgctgaaga ttacctgggt gaaccggtaa ctgaagctgt tatcaccgta
    61201 ccggcatact ttaacgatgc tcagcgtcag gcaaccaaag acgcaggccg tatcgctggt
    61261 ctggaagtaa aacgtatcat caacgaaccg accgcagctg cgctggctta cggtctggac
    61321 aaaggcactg gcaaccgtac tatcgcggtt tatgacctgg gtggtggtac tttcgatatt
    61381 tctattatcg aaatcgacga agttgacggc gaaaaaacct tcgaagttct ggcaaccaac
    61441 ggtgataccc acctgggggg tgaagacttc gacagccgtc tgatcaacta tctggttgaa
    61501 gaattcaaga aagatcaggg cattgacctg cgcaacgatc cgctggcaat gcagcgcctg
    61561 aaagaagcgg cagaaaaagc gaaaatcgaa ctgtcttccg ctcagcagac cgacgttaac
    61621 ctgccataca tcactgcaga cgcgaccggt ccgaaacaca tgaacatcaa agtgactcgt
    61681 gcgaaactgg aaagcctggt tgaagatctg gtaaaccgtt ccattgagcc gctgaaagtt
    61741 gcactgcagg acgctggcct gtccgtatct gatatcgacg acgttatcct cgttggtggt
    61801 cagactcgta tgccaatggt tcagaagaaa gttgctgagt tctttggtaa agagccgcgt
    61861 aaagacgtta acccggacga agctgtagca atcggtgctg ctgttcaggg tggtgttctg
    61921 actggtgacg taaaagacgt actgctgctg gacgttaccc cgctgtctct gggtatcgaa
    61981 accatgggcg gtgtgatgac gacgctgatc gcgaaaaaca ccactatccc gaccaagcac
    62041 agccaggtgt tctctaccgc tgaagacaac cagtctgcgg taaccatcca tgtgctgcag
    62101 ggtgaacgta aacgtgcggc tgataacaaa tctctgggtc agttcaacct agatggtatc
    62161 aacccggcac cgcgcggcat gccgcagatc gaagttacct tcgatatcga tgctgacggt
    62221 atcctgcacg tttccgcgaa agataaaaac agcggtaaag agcagaagat caccatcaag
    62281 gcttcttctg gtctgaacga agatgaaatc cagaaaatgg tacgcgacgc agaagctaac
    62341 gccgaagctg accgtaagtt tgaagagctg gtacagactc gcaaccaggg cgaccatctg
    62401 ctgcacagca cccgtaagca ggttgaagaa gcaggcgaca aactgccggc tgacgacaaa
    62461 actgctatcg agtctgcgct gactgcactg gaaactgctc tgaaaggtga agacaaagcc
    62521 gctatcgaag cgaaaatgca ggaactggca caggtttccc agaaactgat ggaaatcgcc
    62581 cagcagcaac atgcccagca gcagactgcc ggtgctgatg cttctgcaaa caacgcgaaa
    62641 gatgacgatg ttgtcgacgc tgaatttgaa gaagtcaaag acaaaaaata atcgccctat
    62701 aaacgggtaa ttatactgac acgggcgaag gggaatttcc tctccgcccg tgcattcatc
    62761 taggggcaat ttaaaaaaga tggctaagca agattattac gagattttag gcgtttccaa
    62821 aacagcggaa gagcgtgaaa tcagaaaggc ctacaaacgc ctggccatga aataccaccc
    62881 ggaccgtaac cagggtgaca aagaggccga ggcgaaattt aaagagatca aggaagctta
    62941 tgaagttctg accgactcgc aaaaacgtgc ggcatacgat cagtatggtc atgctgcgtt
    63001 tgagcaaggt ggcatgggcg gcggcggttt tggcggcggc gcagacttca gcgatatttt
    63061 tggtgacgtt ttcggcgata tttttggcgg cggacgtggt cgtcaacgtg cggcgcgcgg
    63121 tgctgattta cgctataaca tggagctcac cctcgaagaa gctgtacgtg gcgtgaccaa
    63181 agagatccgc attccgactc tggaagagtg tgacgtttgc cacggtagcg gtgcaaaacc
    63241 aggtacacag ccgcagactt gtccgacctg tcatggttct ggtcaggtgc agatgcgcca
    63301 gggattcttc gctgtacagc agacctgtcc acactgtcag ggccgcggta cgctgatcaa
    63361 agatccgtgc aacaaatgtc atggtcatgg tcgtgttgag cgcagcaaaa cgctgtccgt
    63421 taaaatcccg gcaggggtgg acactggaga ccgcatccgt cttgcgggcg aaggtgaagc
    63481 gggcgagcat ggcgcaccgg caggcgatct gtacgttcag gttcaggtta aacagcaccc
    63541 gattttcgag cgtgaaggca acaacctgta ttgcgaagtc ccgatcaact tcgctatggc
    63601 ggcgctgggt ggcgaaatcg aagtaccgac ccttgatggt cgcgtcaaac tgaaagtgcc
    63661 tggcgaaacc cagaccggta agctattccg tatgcgcggt aaaggcgtca agtctgtccg
    63721 cggtggcgca cagggtgatt tgctgtgccg cgttgtcgtc gaaacaccgg taggcctgaa
    63781 cgaaaggcag aaacagctgc tgcaagagct gcaagaaagc ttcggtggcc caaccggcga
    63841 gcacaacagc ccgcgctcaa agagcttctt tgatggtgtg aagaagtttt ttgacgacct
    63901 gacccgctaa cctccccaaa agcctgcccg tgggcaggcc tgggtaaaaa tagggtgcgt
    63961 tgaagatatg cgagcacctg taaagtggcg gggat
//
LOCUS       DBBGLQ010000025        63519 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.025, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000025 DBBGLQ010000000
VERSION     DBBGLQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.025"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            379..465
                     /gene="leuL"
                     /locus_tag="ACIYSB_001957"
     CDS             379..465
                     /gene="leuL"
                     /locus_tag="ACIYSB_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGC5429027.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            558..2129
                     /gene="leuA"
                     /locus_tag="ACIYSB_001958"
     CDS             558..2129
                     /gene="leuA"
                     /locus_tag="ACIYSB_001958"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGC5429028.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITEYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2129..3220
                     /gene="leuB"
                     /locus_tag="ACIYSB_001959"
     CDS             2129..3220
                     /gene="leuB"
                     /locus_tag="ACIYSB_001959"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGC5429029.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3223..4623
                     /gene="leuC"
                     /locus_tag="ACIYSB_001960"
     CDS             3223..4623
                     /gene="leuC"
                     /locus_tag="ACIYSB_001960"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGC5429030.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4634..5239
                     /gene="leuD"
                     /locus_tag="ACIYSB_001961"
     CDS             4634..5239
                     /gene="leuD"
                     /locus_tag="ACIYSB_001961"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGC5429031.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5288..6466)
                     /gene="setA"
                     /locus_tag="ACIYSB_001962"
     CDS             complement(5288..6466)
                     /gene="setA"
                     /locus_tag="ACIYSB_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HGC5429032.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFNSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6568..6699)
                     /gene="sgrT"
                     /locus_tag="ACIYSB_001963"
     CDS             complement(6568..6699)
                     /gene="sgrT"
                     /locus_tag="ACIYSB_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGC5429033.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6788..8443
                     /gene="sgrR"
                     /locus_tag="ACIYSB_001964"
     CDS             6788..8443
                     /gene="sgrR"
                     /locus_tag="ACIYSB_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGC5429034.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQNTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLKIKEIDYDQWHTGEIES
                     DIWLNSANFTLPLDFSVFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLLHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8477..8576
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8607..9590
                     /gene="thiB"
                     /locus_tag="ACIYSB_001965"
     CDS             8607..9590
                     /gene="thiB"
                     /locus_tag="ACIYSB_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGC5429035.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9566..11176
                     /gene="thiP"
                     /locus_tag="ACIYSB_001966"
     CDS             9566..11176
                     /gene="thiP"
                     /locus_tag="ACIYSB_001966"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGC5429036.1"
                     /translation="MATRRQPLIPGWLIPGVSATTLVVAVALAAFLALWWNAPQDDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVIPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLATLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRSWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQVLEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11160..11858
                     /gene="thiQ"
                     /locus_tag="ACIYSB_001967"
     CDS             11160..11858
                     /gene="thiQ"
                     /locus_tag="ACIYSB_001967"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGC5429037.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTMPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQGKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(11972..12736)
                     /gene="yabI"
                     /locus_tag="ACIYSB_001968"
     CDS             complement(11972..12736)
                     /gene="yabI"
                     /locus_tag="ACIYSB_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGC5429038.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIIGCLMGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12822..13700)
                     /gene="araC"
                     /locus_tag="ACIYSB_001969"
     CDS             complement(12822..13700)
                     /gene="araC"
                     /locus_tag="ACIYSB_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGC5429039.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQARLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14039..15739
                     /gene="araB"
                     /locus_tag="ACIYSB_001970"
     CDS             14039..15739
                     /gene="araB"
                     /locus_tag="ACIYSB_001970"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGC5429040.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKTQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15750..17252
                     /gene="araA"
                     /locus_tag="ACIYSB_001971"
     CDS             15750..17252
                     /gene="araA"
                     /locus_tag="ACIYSB_001971"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGC5429041.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGKKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17537..18232
                     /gene="araD"
                     /locus_tag="ACIYSB_001972"
     CDS             17537..18232
                     /gene="araD"
                     /locus_tag="ACIYSB_001972"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGC5429042.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18307..20658
                     /gene="polB"
                     /locus_tag="ACIYSB_001973"
     CDS             18307..20658
                     /gene="polB"
                     /locus_tag="ACIYSB_001973"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGC5429043.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20823..23729
                     /gene="rapA"
                     /locus_tag="ACIYSB_001974"
     CDS             20823..23729
                     /gene="rapA"
                     /locus_tag="ACIYSB_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGC5429044.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23741..24400
                     /gene="rluA"
                     /locus_tag="ACIYSB_001975"
     CDS             23741..24400
                     /gene="rluA"
                     /locus_tag="ACIYSB_001975"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGC5429045.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24613..24791
                     /locus_tag="ACIYSB_001976"
                     /pseudo
     CDS             24613..24791
                     /locus_tag="ACIYSB_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(24808..24966)
                     /gene="yabQ"
                     /locus_tag="ACIYSB_001977"
     CDS             complement(24808..24966)
                     /gene="yabQ"
                     /locus_tag="ACIYSB_001977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YabQ"
                     /protein_id="HGC5429046.1"
                     /translation="MNGATSLYDEVIIINKIPPKKIDTKGVATEEVATKKVLLNKLLT
                     TQLLNEPE"
     gene            complement(24963..25614)
                     /gene="yabP"
                     /locus_tag="ACIYSB_001978"
                     /pseudo
     CDS             complement(24963..25614)
                     /gene="yabP"
                     /locus_tag="ACIYSB_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YabP"
     gene            complement(25909..26724)
                     /gene="djlA"
                     /locus_tag="ACIYSB_001979"
     CDS             complement(25909..26724)
                     /gene="djlA"
                     /locus_tag="ACIYSB_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGC5429047.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26979..29333
                     /gene="lptD"
                     /locus_tag="ACIYSB_001980"
     CDS             26979..29333
                     /gene="lptD"
                     /locus_tag="ACIYSB_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGC5429048.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDARIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            29386..30672
                     /gene="surA"
                     /locus_tag="ACIYSB_001981"
     CDS             29386..30672
                     /gene="surA"
                     /locus_tag="ACIYSB_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGC5429049.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30672..31661
                     /gene="pdxA"
                     /locus_tag="ACIYSB_001982"
     CDS             30672..31661
                     /gene="pdxA"
                     /locus_tag="ACIYSB_001982"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGC5429050.1"
                     /translation="MVKTQRVVITPGEPAGSGPDLVVQLAQREWPVELVVCADATLLT
                     NRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRAPVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            31658..32479
                     /gene="rsmA"
                     /locus_tag="ACIYSB_001983"
     CDS             31658..32479
                     /gene="rsmA"
                     /locus_tag="ACIYSB_001983"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGC5429051.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            32482..32859
                     /gene="apaG"
                     /locus_tag="ACIYSB_001984"
     CDS             32482..32859
                     /gene="apaG"
                     /locus_tag="ACIYSB_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGC5429052.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32866..33708
                     /gene="apaH"
                     /locus_tag="ACIYSB_001985"
     CDS             32866..33708
                     /gene="apaH"
                     /locus_tag="ACIYSB_001985"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGC5429053.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(33786..34265)
                     /gene="folA"
                     /locus_tag="ACIYSB_001986"
     CDS             complement(33786..34265)
                     /gene="folA"
                     /locus_tag="ACIYSB_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGC5429054.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVLGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34457..36319)
                     /gene="kefC"
                     /locus_tag="ACIYSB_001987"
     CDS             complement(34457..36319)
                     /gene="kefC"
                     /locus_tag="ACIYSB_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGC5429055.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     VSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36312..36842)
                     /gene="kefF"
                     /locus_tag="ACIYSB_001988"
     CDS             complement(36312..36842)
                     /gene="kefF"
                     /locus_tag="ACIYSB_001988"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGC5429056.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRPLEWQEAHHG"
     gene            complement(36950..38281)
                     /gene="yaaU"
                     /locus_tag="ACIYSB_001989"
     CDS             complement(36950..38281)
                     /gene="yaaU"
                     /locus_tag="ACIYSB_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5429057.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38339..38626)
                     /gene="fixX"
                     /locus_tag="ACIYSB_001990"
     CDS             complement(38339..38626)
                     /gene="fixX"
                     /locus_tag="ACIYSB_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGC5429058.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38623..39909)
                     /gene="fixC"
                     /locus_tag="ACIYSB_001991"
     CDS             complement(38623..39909)
                     /gene="fixC"
                     /locus_tag="ACIYSB_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGC5429059.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39960..40901)
                     /gene="fixB"
                     /locus_tag="ACIYSB_001992"
     CDS             complement(39960..40901)
                     /gene="fixB"
                     /locus_tag="ACIYSB_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGC5429060.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40916..41686)
                     /gene="fixA"
                     /locus_tag="ACIYSB_001993"
     CDS             complement(40916..41686)
                     /gene="fixA"
                     /locus_tag="ACIYSB_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGC5429061.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            42155..43669
                     /gene="caiT"
                     /locus_tag="ACIYSB_001994"
     CDS             42155..43669
                     /gene="caiT"
                     /locus_tag="ACIYSB_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGC5429062.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43700..44842
                     /gene="caiA"
                     /locus_tag="ACIYSB_001995"
     CDS             43700..44842
                     /gene="caiA"
                     /locus_tag="ACIYSB_001995"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGC5429063.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44971..46188
                     /gene="caiB"
                     /locus_tag="ACIYSB_001996"
     CDS             44971..46188
                     /gene="caiB"
                     /locus_tag="ACIYSB_001996"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGC5429064.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLATHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46262..47815
                     /gene="caiC"
                     /locus_tag="ACIYSB_001997"
     CDS             46262..47815
                     /gene="caiC"
                     /locus_tag="ACIYSB_001997"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGC5429065.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLCEESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDQQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRVGFCYEAEIRDDHNRPLPAGEIGEICIKGIPGKTIFKEYFLNPQATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIAAHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47924..48709
                     /gene="caiD"
                     /locus_tag="ACIYSB_001998"
     CDS             47924..48709
                     /gene="caiD"
                     /locus_tag="ACIYSB_001998"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGC5429066.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48715..49305
                     /gene="caiE"
                     /locus_tag="ACIYSB_001999"
     CDS             48715..49305
                     /gene="caiE"
                     /locus_tag="ACIYSB_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGC5429067.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCLIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRNVSDDELHWKRLNTKEYQ
                     DLVGRCHVSLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49391..49786)
                     /gene="caiF"
                     /locus_tag="ACIYSB_002000"
     CDS             complement(49391..49786)
                     /gene="caiF"
                     /locus_tag="ACIYSB_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGC5429068.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(50048..53269)
                     /gene="carB"
                     /locus_tag="ACIYSB_002001"
     CDS             complement(50048..53269)
                     /gene="carB"
                     /locus_tag="ACIYSB_002001"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGC5429069.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGADRIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53287..54435)
                     /gene="carA"
                     /locus_tag="ACIYSB_002002"
     CDS             complement(53287..54435)
                     /gene="carA"
                     /locus_tag="ACIYSB_002002"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGC5429070.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(54891..55712)
                     /gene="dapB"
                     /locus_tag="ACIYSB_002003"
     CDS             complement(54891..55712)
                     /gene="dapB"
                     /locus_tag="ACIYSB_002003"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGC5429071.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(55879..56793)
                     /gene="rihC"
                     /locus_tag="ACIYSB_002004"
     CDS             complement(55879..56793)
                     /gene="rihC"
                     /locus_tag="ACIYSB_002004"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGC5429072.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIL
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(56859..57809)
                     /gene="ispH"
                     /locus_tag="ACIYSB_002005"
                     /gene_synonym="lytB"
     CDS             complement(56859..57809)
                     /gene="ispH"
                     /locus_tag="ACIYSB_002005"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGC5429073.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(57811..58260)
                     /gene="fkpB"
                     /locus_tag="ACIYSB_002006"
     CDS             complement(57811..58260)
                     /gene="fkpB"
                     /locus_tag="ACIYSB_002006"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGC5429074.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(58385..58879)
                     /gene="lspA"
                     /locus_tag="ACIYSB_002007"
     CDS             complement(58385..58879)
                     /gene="lspA"
                     /locus_tag="ACIYSB_002007"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGC5429075.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(58879..61695)
                     /gene="ileS"
                     /locus_tag="ACIYSB_002008"
     CDS             complement(58879..61695)
                     /gene="ileS"
                     /locus_tag="ACIYSB_002008"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGC5429076.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(61738..62679)
                     /gene="ribF"
                     /locus_tag="ACIYSB_002009"
     CDS             complement(61738..62679)
                     /gene="ribF"
                     /locus_tag="ACIYSB_002009"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGC5429077.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(62687..62905)
                     /gene="yaaY"
                     /locus_tag="ACIYSB_002010"
     CDS             complement(62687..62905)
                     /gene="yaaY"
                     /locus_tag="ACIYSB_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGC5429078.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            63008..63271
                     /gene="rpsT"
                     /locus_tag="ACIYSB_002011"
     CDS             63008..63271
                     /gene="rpsT"
                     /locus_tag="ACIYSB_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGC5429079.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        15256 a        17297 c        16457 g        14509 t
ORIGIN      
        1 aataattaat ttatatattt aatgcattaa ttcttaacat taattgatca ataatattca
       61 ccaaatcaat atcaaaaaaa atcgcaaaac atataattca atacaaatca tcaggatagg
      121 ttttgcaacg cgtgcatttt gtcccctttt tcctcgttga ttagatgcaa aaatttatgc
      181 tgaaatatgt caaccgatga aaagcgtcgg tagttaagca gaaattaata tcgcttactt
      241 taaccaccgc agcacaatta gctaatttta cggatgcaga actcacgctg gcgggacgtt
      301 tttattgcgt cagggttgac atccgttttt gtatccagta actctaaaag catatcgcat
      361 tcatctggag ctgatttaat gactcacatc gttcgcttta tcggtctact actactaaac
      421 gcatcttctt tgcgcggtag acgagtgagc ggcatccagc attaagccag cacgcagtca
      481 aacaaaaaac ccgcgccatt gcgcgggttt ttttatgccc gaagcgaggc gctctaaaag
      541 agacaaggac ccaaaccatg agccagcaag tcattatttt cgataccaca ttgcgcgacg
      601 gtgaacaggc gttacaggca agcttgagtg tgaaagaaaa actgcaaatt gcgctggccc
      661 ttgagcgtat gggtgttgac gtgatggaag tcggtttccc cgtctcttcg ccgggcgatt
      721 ttgaatcggt gcaaaccatc gcccgccagg ttaaaaacag ccgcgtatgt gcgttagctc
      781 gctgcgtgga aaaagatatc gacgtggcgg ccgaatccct gaaagtcgcc gaagccttcc
      841 gtattcatac ctttattgcc acttcgccaa tgcacatcgc caccaagctg cgcagcacgc
      901 tggacgaggt gatcgaacgc gctatctata tggtgaaacg cgcccgtaat tacaccgatg
      961 atgttgaatt ttcttgcgaa gatgccgggc gtacacccat tgccgatctg gcgcgagtgg
     1021 tcgaagcggc gattaatgcc ggtgccacta ccatcaacat tccggacacc gtgggctaca
     1081 ccatgccgtt tgagttcgcc ggaatcatca gcggcctgta tgaacgcgtg cctaacatcg
     1141 acaaagccat tatctccgta catacccacg acgatttggg cctggcggtc ggaaactcac
     1201 tggcggcggt acatgccggt gcacgccagg tggaaggcgc aatgaacggg atcggcgagc
     1261 gtgccggaaa ctgttccctg gaagaagtca tcatggcgat caaagttcgt aaggatattc
     1321 tcaacgtcca caccgccatt aatcaccagg agatatggcg caccagccag ttagttagcc
     1381 agatttgtaa tatgccgatc ccggcaaaca aagccattgt tggcagcggc gcattcgcac
     1441 actcctccgg tatacaccag gatggcgtgc tgaaaaaccg cgaaaactac gaaatcatga
     1501 caccagaatc tattggtctg aaccaaatcc agctgaatct gacctctcgt tcggggcgtg
     1561 cggcggtgaa acatcgcatg gatgagatgg ggtataaaga aagtgaatat aatttagaca
     1621 atttgtacga tgctttcctg aagctggcgg acaaaaaagg tcaggtgttt gattacgatc
     1681 tggaggcgct ggccttcatc ggtaagcagc aagaagagcc ggagcatttc cgtctggatt
     1741 acttcagcgt gcagtctggc tctaacgata tcgccaccgc cgccgtcaaa ctggcctgtg
     1801 gcgaagaagt caaagcagaa gccgccaacg gtaacggtcc ggtcgatgcc gtctatcagg
     1861 caattaaccg catcactgaa tataacgtcg aactggtgaa atacagcctg accgccaaag
     1921 gccacggtaa agatgcgctg ggtcaggtgg atatcgtcgc taactacaac ggtcgccgct
     1981 tccacggcgt cggcctggct accgatattg tcgagtcatc tgccaaagcc atggtgcacg
     2041 ttctgaacaa tatctggcgt gccgcagaag tcgaaaaaga gttgcaacgc aaagctcaac
     2101 acaacgaaaa caacaaggaa accgtgtgat gtcgaagaat taccatattg ccgtattgcc
     2161 gggggacggt attggtccgg aagtgatgac ccaggcgctg aaagtgctgg atgccgtgcg
     2221 caaccgcttt gcgatgcgca tcaccaccag ccattacgat gtaggcggcg cagccattga
     2281 taaccacggg caaccactgc cgcctgcgac ggttgaaggt tgtgagcaag ccgatgccgt
     2341 gctgtttggc tcggtaggcg gcccgaagtg ggaacattta ccaccagacc agcaaccaga
     2401 acgcggcgcg ctgctgcctc tgcgtaagca cttcaaatta ttcagcaacc tgcgcccggc
     2461 aaaactgtat caggggctgg aagcattctg tccgctgcgt gcagacattg ccgcaaacgg
     2521 cttcgacatc ctgtgtgtgc gcgaactgac cggcggcatc tatttcggtc agccaaaagg
     2581 ccgcgaaggt agcggacaat atgaaaaagc ctttgatacc gaggtgtatc accgttttga
     2641 gatcgaacgt atcgcccgca tcgcgtttga atctgctcgc aagcgtcgcc acaaagtgac
     2701 gtcgatcgat aaagccaacg tgctgcaatc ctctatttta tggcgggaga tcgttaacga
     2761 gatcgccacg gaatacccgg atgtcgaact ggcgcatatg tacatcgaca acgccaccat
     2821 gcagctgatt aaagatccat cacagtttga cgttctgctg tgctccaacc tgtttggcga
     2881 cattctgtct gacgagtgcg caatgatcac tggctcgatg gggatgttgc cttccgccag
     2941 cctgaacgag caaggttttg gactgtatga accggcgggc ggctcggcac cagatatcgc
     3001 aggcaaaaac atcgccaacc cgattgcaca aatcctttcg ctggcactgc tgctgcgtta
     3061 cagcctggat gccgatgatg cggcttgcgc cattgaacgc gccattaacc gcgcattaga
     3121 agaaggcatt cgcaccgggg atttagcccg tggcgctgcc gccgttagta ccgatgaaat
     3181 gggcgatatc attgcccgct atgtagcaga aggggtgtaa tcatggctaa gacgttatac
     3241 gaaaaattgt tcgacgctca cgttgtgtac gaagccgaaa acgaaacccc actgttatat
     3301 atcgaccgcc acctggtgca tgaagtgacc tcaccgcagg cgttcgatgg tctgcgcgcc
     3361 cacggtcgcc cggtacgtca gccgggcaaa accttcgcta ccatggatca caacgtctct
     3421 acccagacca aagacattaa tgcctgcggt gaaatggcgc gtatccagat gcaggaactg
     3481 atcaaaaact gcaaagaatt tggcgtcgaa ctgtatgacc tgaatcaccc gtatcagggg
     3541 atcgtccacg taatggggcc ggaacagggc gtcaccttgc cggggatgac cattgtctgc
     3601 ggcgactcgc ataccgccac ccacggcgcg tttggcgcac tggcctttgg tatcggcact
     3661 tccgaagttg aacacgtact ggcaacgcaa accctgaaac agggccgcgc aaaaaccatg
     3721 aaaattgaag tccagggcaa agccgcgccg ggcattaccg caaaagatat cgtgctggca
     3781 attatcggta aaaccggtag cgcaggcggc accgggcatg tggtggagtt ttgcggcgaa
     3841 gcaatccgtg atttaagcat ggaaggtcgt atgaccctgt gcaatatggc aatcgaaatg
     3901 ggcgcaaaag ccggtctggt tgcaccggac gaaaccacct ttaactatgt caaaggccgt
     3961 ctgcatgcgc cgaaaggcaa agatttcgac gacgccgttg cctactggaa aaccctgcaa
     4021 accgacgaag gcgcaacttt cgataccgtt gtcactctgc aagcagaaga aatttcaccg
     4081 caggtcacct ggggcaccaa tcccggccag gtgatttccg tgaacgacaa tattcccgat
     4141 ccggcttcgt ttgccgatcc ggttgaacgc gcgtcggcag aaaaagcgct ggcctatatg
     4201 gggctgaaac cgggtattcc gctgaccgaa gtggctatcg acaaagtgtt tatcggttcc
     4261 tgtaccaact cgcgcattga agatttacgc gcggcagcgg agatcgccaa agggcgaaaa
     4321 gtcgcgccag gcgtgcaggc actggtggtt cccggctctg gcccggtaaa agcccaggcg
     4381 gaagcggaag gtctggataa aatctttatt gaagccggtt ttgaatggcg cttgcctggc
     4441 tgctcaatgt gtctggcgat gaacaacgac cgtctgaatc cgggcgaacg ttgtgcctcc
     4501 accagcaacc gtaactttga aggccgccag gggcgcggcg ggcgcacgca tctggtcagc
     4561 ccggcaatgg ctgccgctgc tgctgtgacc ggacatttcg ccgacattcg caacattaaa
     4621 taaggagcac accatggcag agaaatttat caaacacaca ggcctggtgg ttccgctgga
     4681 tgccgccaat gtcgataccg atgcaatcat cccgaaacag tttttgcaga aagtgacccg
     4741 tacgggtttt ggcgcgcatc tgtttaacga ctggcgtttt ctggatgaaa aaggccaaca
     4801 gccaaacccg gacttcgtgc tgaacttccc gcagtatcag ggcgcttcca ttttgctggc
     4861 acgagaaaac ttcggctgtg gctcttcgcg tgagcacgcg ccctgggcat tgaccgacta
     4921 cggttttaaa gtggtgattg cgccgagttt tgctgacatc ttctacggca atagctttaa
     4981 caaccagctg ctgccggtga aattaagcga tgcagaagtg gacgaactgt ttgcgctggt
     5041 gaaagctaat ccggggatcc atttcgacgt ggatctggaa gcgcaagagg tgaaagcggg
     5101 agagaaaacc tatcgcttta ccatcgatgc cttccgccgc cactgcatga tgaacggtct
     5161 ggacagtatt gggcttacct tgcagcacga cgacgccatt gccgcttatg aagcaaaaca
     5221 acctgcgttt atgaattaat ccccttgccc ggtcaaatga ccgggctttc cgctatcgtc
     5281 cacgtcatca aacgtcttta acctttgcgg ttaaaaataa tgcgacaaca gaaataaccg
     5341 caattaccca gtagacagca aagtgccccc aactttgtgc aattgctccc tgaataacgc
     5401 cagccagaat taccccggta gaaatactgt tagtaaataa ggtggtagct gcccccgctc
     5461 ttccaggcat taaatcctga aaccatagca tcccaatacc cgcaacaatg ccgataaata
     5521 cagcgttaaa aagttgcagc gtcatcaacg ccatacggct attaaagaaa atcaatccgg
     5581 tgtaaaacag tactcctgcc gccactgcta tgaccatcat tcgccgctta ccataacgtt
     5641 tgacatagta gccagccaga atcattgctg gtatttccag tccagctgcc gtccccatca
     5701 ggaaacccgc cagtttgtct ggcaatccta actcgctact gatccacaac ggcatatcaa
     5761 taatgtacat ggtgttgcag gtccacatta acgtcgaggc gacaaataac atccgtacgt
     5821 tactatcctg ccagccacct tgcattgata aagcattttc cgacggcagt tctacccgcg
     5881 ccacagacgg aagcataaat gcaatcaata ccagactgag tgtgaatatc ccggcggcaa
     5941 tcgaaaacat caccgtaaag ccgtaattca acgccagcat aaaggccaac ggtggaccga
     6001 taacccatgc cagagaaagc tgcgcacgca tcaccgagct aaacatcacc acttctcgcg
     6061 ccgagttatc cgcatattcc cgcgccagag caaataactg tggcattgcc gtattggcca
     6121 gagatgccag aagcacacca caggtgataa gcgtcagata atgacgatta aatgcaaaca
     6181 atagcgcatt gccgatagcc atcaaacagc aaaatataat cagttttcgc cgatcgccct
     6241 gactgtcaga acgttttgcc aaccagaggc ttacgccgat cccagcaata gcattcaccg
     6301 tataaaagag gccgatccag aaaggttgcg cgccaacctc acgactcaga aataagctca
     6361 atgtaggagc ctgtagcgcc ccggccaccc ccatcataaa agcgaccagc ataaatgccg
     6421 cgtaaacacc gttcatacgg cgagccatcg tcattatcca gatcatacgt tcccttttta
     6481 gcgcggcgag aataaaaaaa accagcaggt ataatctgct ggcgggtgat tttacaccaa
     6541 tactcagtca cacatgatgc aggcaagtca actttcagaa ttgcggtcat cccactgcat
     6601 cagtccttca agcatggtta atcgttgtgg gacgcttaac caacgcaacc agcacaactt
     6661 cgctgtcgcg gtaaaatagt gctgataaaa ctgacgcatg gggcaccccc ttgcttcatc
     6721 gttaggaatt attgacttaa tatagggaaa ataaaattgc tgtcttttgc acaggagttc
     6781 cccttttatg ccatctgctc gtctgcaaca acagttcatc cgcctgtggc aatgctgcga
     6841 gggtaaatcg cagaacacaa cgctcaacga actggcagcg ttattgagct gctcgcgtcg
     6901 tcatatgcgc accctgctca acaccatgca ggatcgcggc tggctgacgt gggaagcgga
     6961 agtcgggcgc ggtaaacgct cgcgtctgac attcctctat accgggctgg cgcttcagca
     7021 acagcgggcg gaagacctgc tggagcagga tcgtatcgat caactggtgc agttagttgg
     7081 cgacaaagcg actgtgcggc aaatgctggt ttctcatctg ggccgcagct tccgccaggg
     7141 gcggcacatc ctgcgcgtgc tctactatcg tccgttgcgt aatctgctac ctggcagcgc
     7201 attgcgccgt tccgaaaccc atatcgcccg gcaaatcttc agttcgctaa cgcgcataaa
     7261 tgaggaaaat ggggaactgg aagcagacat cgcccaccac tggcagcaaa tttcaccgct
     7321 tcactggcgt ttctttttgc gtccaggagt ccattttcac catggtcgtg aactggaaat
     7381 ggacgatgtg atcgcctctt taaaacgaat caatacgctg ccgctctatt cgcatattgc
     7441 tgacattgtg tcgccgacgc cctggacgct ggatatccat ctcacgcaac cggaccgctg
     7501 gttaccgtta ctgctggggc aagttccggc gatgatcctg ccgcgcgaat gggaaaccct
     7561 cagtaacttt gccagccatc ccatcggcac cggtccgtat gcggtgattc gcaacagcac
     7621 caatcaactg aaaattcagg cattcgatga cttcttcggt taccgggcat taatcgacga
     7681 agttaacgtc tgggttctgc cggaaattgc cgacgagcca gccggagggc tgatgctaaa
     7741 aggtccacag ggcgaggaaa aagagattga aagccgcctg gaggaaggtt gctactattt
     7801 actgttcgac agccgcaccc atcgcggggc gaatcagcaa gtcagggact gggtaagcta
     7861 tgtgctttct ccaactaatc tggtctattt cgctgaggaa cagtaccagc aactgtggtt
     7921 cccggcttat ggactgctcc cccgttggca ccatgcccgc accataaaga gcgaaaaacc
     7981 ggctggcctg gaaagcctca ccctaacctt ttatcaggat cacagtgagc atcgggtgat
     8041 tgccgggatc atgcagcaga ttctggcaag tcaccaggtc acgctgaaaa tcaaagagat
     8101 cgactacgat cagtggcata caggagagat cgaaagtgat atctggctaa acagcgccaa
     8161 ctttaccctg ccgctggact tctctgtttt cgcacattta tgcgaagtgc cactgctaca
     8221 acattgcatt cccattgact ggcaagccga cgctgctcgc tggcgcaatg gcgagatgaa
     8281 tctggcgaac tggtgccagc aactggtcgc cagcaaagcg atggtgccat tattgcacca
     8341 ctggctgatc attcaggggc aacgcagtat gcgcggcctg cgcatgaata ccctcggctg
     8401 gttcgatttt aaatcagcgt ggtttgcgcc accggatcca tgattgctgg ttgataacaa
     8461 aatcactaca ctaacgccgt tctcaacggg gtgccacgcg tacgcgtgcg ctgagaaaat
     8521 acccgtcgaa cctgatccgg ataacgccgg cgaagggatt tgaggctcct tctcaagtcc
     8581 tttgccactc ttttttgagg tgcaaagtgt taaaaaaatg tctgcccctg ctgttgctgt
     8641 gcacagcgcc cgttttcgct aaacccgttc tgactgttta tacctacgat tccttcgccg
     8701 ccgactgggg gcctggtccg gtggttaaaa aagcctttga agccgactgt aattgcgaac
     8761 tgaaactggt ggcgctggaa gatggcgttt cgcttctcaa ccgtctacgg atggaaggca
     8821 aaaacagtaa agccgatgtg gtgctggggc tggataacaa cctgttagac gccgccagta
     8881 aaaccggact gtttgccaaa agcggtgtgg cagcggatgc cgttaacgtt cccggcggct
     8941 ggaataatga cactttcgta ccgtttgatt atggctactt cgccttcgtt tatgacaaga
     9001 acaaactgaa aaacccgcca caaagcctga aagaactggt tgagagcgat caaaactggc
     9061 gggtgattta tcaggatccg cgcaccagta caccggggct gggtctgttg ctatggatgc
     9121 aaaaagtcta tggcgatgac gccccacaag cctggcagaa actggcgaag aaaacggtca
     9181 cggtcaccaa aggctggagc gaagcctacg gcctgttttt aaaaggtgaa agcgatctgg
     9241 tactgagtta caccacctct ccggcttatc acattctcga agagaagaaa gataactacg
     9301 ccgccgcgaa cttcagcgaa ggtcactatc tgcaagtgga agtcgccgcc cgcaccgctg
     9361 ccagcaagca gccggagctg gcgcaaaaat tcctccagtt tatggtttct ccggctttcc
     9421 agaatgcgat cccaaccggc aactggatgt atccggtggc aaacgtcacg ctgcctgccg
     9481 gttttgaaaa attgaccaaa cccgcaacca cgttggagtt cacgccagcc gaagtggcgg
     9541 cacaacgtca ggcatggatt agcgaatggc aacgcgccgt cagccgttaa ttcccggctg
     9601 gttaattcca ggtgtaagcg ccaccacgct ggtggtagcg gttgcgctgg cggcgtttct
     9661 cgccctgtgg tggaacgcgc cgcaggatga ctgggtggca gtctggcagg acagctatct
     9721 gtggcatgtg gtgcgcttct ccttctggca ggcgtttctc tcggcactgc tctctgtcat
     9781 acccgcgata ttcctcgccc gcgcgctcta tcgcaggcgc tttccgggtc ggctggcgct
     9841 gttgcgtctg tgtgcaatga ccttgatcct cccggtgttg gtcgctgttt tcggcattct
     9901 tagcgtctat ggtcgccagg gctggctggc aacactctgc caatcgctcg gtctggagtg
     9961 gaccttttcg ccctacggcc tgcaaggtat tttgctggcc catgtgtttt ttaatctgcc
    10021 gatggcgagc cgcttattac tccaggcact ggaaaacatc cccggcgaac agcgtcaact
    10081 tgccgcccag cttgggatgc gtagctggca ttttttccgc ttcgtcgaat ggccgtggtt
    10141 acggcgacaa atcccgccgg ttgctgcgct tatctttatg ctctgtttcg ccagcttcgc
    10201 caccgtgcta tcgctggggg gcggtccgca ggcgaccact atcgagctgg caatctatca
    10261 ggcgctgagt tacgactacg atcctgcccg cgcggcaatg ctggcgctgc tccagatggt
    10321 gtgctgcctc gggctggtgc tgttgagtca gcgattgagt aaggccattg cgcccggcac
    10381 cacgctgctg caaggctggc gcgacccgga cgatcgtctg catagccgca tttgcgacac
    10441 ggtgttaatt gtgctggcgc tgctgctgtt gctgccaccg ttactggcgg tgatcgtcga
    10501 tggggtaaat cgccagttgc cggaagtgct ggcacaaccg gtgctgtggc aggcgctgtg
    10561 gacctcgttg cgtattgcgc tggcggcagg tgtattgtgc gtagtgctga ccatgatgct
    10621 gctatggagc agtcgcgaac tgcgggcgcg gcagaaaatg ctggcgggtc aggtgctgga
    10681 gatgagcggc atgttgatcc tcgccatgcc ggggattgtg ctggctaccg gcttcttttt
    10741 actgctcaac aacactatcg gcctgccaca atctgctgac ggcattgtga ttttcaccaa
    10801 tgcgttaatg gcgatccctt atgcgctgaa agtgctggaa aacccgatgc gcgatatcac
    10861 cgcccgctac agcatgttat gtcagtcgct ggggattgaa ggctggtcac gcttaaaagt
    10921 ggtggagctg cgcgccctga aacgtccact ggcgcaggcg ctggcctttg catgcgtgct
    10981 gtcgattggt gattttggcg tggtggcgtt gttcggtaac gatgatttcc gcaccctgcc
    11041 gttttatctc taccagcaaa ttggctccta tcgcagccag gacggtgcgg tcaccgcgtt
    11101 aattctgctg ctgctctgtt ttctgctgtt taccgtgatt gaaaaactac cggggcgaaa
    11161 tgttaaaact gactgatatc acctggcttt accaccattt gccgatgcgt tttagcttaa
    11221 cggtggaacg cggcgagcag gtggcgatcc tcgggccaag cggcgcgggt aaaagtaccc
    11281 tgctgaattt gatcgccggt tttctgacgc cagccagcgg ttcgctgact atcgatggcg
    11341 tagatcacac aactatgccg ccgtcacgcc gtccggtgtc gatgctgttt caggagaaca
    11401 acctgttcag ccacctgacg gtcgcacaga acatcgggct ggggctaaat ccgggattga
    11461 aactgaacgc ggtacagcag gggaaaatgc acgctatcgc ccgccagatg gggattgata
    11521 atttaatggc gcggttaccg ggcgagcttt ccggcggtca gcgacagcga gtggcgttag
    11581 cgcgttgtct ggtacgcgaa cagccgattt tattgctcga tgaaccgttc tctgcgctcg
    11641 atccggcgtt acgtcaggag atgttgacgc tggtgagcac gagctgccag cagcaaaaaa
    11701 tgacgctatt gatggtgtcg cacagcgtgg aagatgcggc gcggatcgcc acgcgctcgg
    11761 tagtagtcgc cgacgggcgc atcgcctggc agggtatgac caatgagttg ttgagcggta
    11821 aggcgagtgc ttcggcacta ttggggatta cgggttagta tgcactgtcg gatgcggcgt
    11881 gaacgcctta tccgacctac gggtagtgcg ttttgtaggc ctgataagac gcggcaagcg
    11941 tcgcatcagg caacccgcac aagactatct cctaaacccc aaccacttta cgcaaaatat
    12001 cgatatacac cggcatcaac gggtggcgaa ttaacaccac cagcgccacc acgccgatgg
    12061 cagaaatcaa cggcgtcagc cacaacaaac gaccgcggga caaataatga ctcaaacggt
    12121 cagtcgcttt accgctgcgc cataaccgcc agcacagcca gccaccaacc cacaaaaaca
    12181 ccgctgttgc cagcagcaac catttaaact caccgctctg cattccggca ggaatatcga
    12241 tcgccgcgcc cgccagaatc cctggcagga agtaaaacgg cggccacagc aggcagccga
    12301 taatattcgg cgtaataaat ttagccaccg gcagatccag cattcccgcc accattggca
    12361 ccagcggacg cgtcgggcca acaaaacgac cgaccagaat ggtgaacatg ctgtgttgat
    12421 gcaacgcatg ttcagtttta tcaagtagtg ctttgttttt cttcagaaat gaccagcgat
    12481 gcaacggctt tttaaaacgc caacccagcc agaaagaaat ccagtcgccc atcaagcagc
    12541 caataatccc tgccagccag gcgtgccaga aacttaactc gccgctgcca atcagcgctc
    12601 ccagccccgc catcagcacc gtaccgggta gaatcaaacc gaccagcgcc agcgactcca
    12661 gaaaggccac caacaccacc gccatcaatg aatacacggt ggattgggta ataaagtgtt
    12721 ccagcaatgc ttgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta
    12781 tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct
    12841 acatcattca ctttttcttc acaaccggca cgaaactcgc tcgggctggc cccggtgcat
    12901 tttttaaata ctcgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
    12961 gcgataggca tccgggtagt gctcaaaagc agcctcgcct gactaatgcg ttggtcctcg
    13021 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc
    13081 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg
    13141 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta
    13201 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa
    13261 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc
    13321 tggtgcgctt catccgggcg aaagaaaccc gtattggcaa atattgacgg ccagttaagc
    13381 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc
    13441 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt
    13501 cggcagacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg
    13561 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc
    13621 tcaatcggcg ttaaacccgc caccagatgg gcgttaaacg agtatcccgg cagcagggga
    13681 tcattttgcg cttcagccat acttttcata ctcccaccat tcagagaaga aaccaattgt
    13741 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaacccaa
    13801 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa
    13861 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc
    13921 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc
    13981 tttttatcgc aactctctac tgtttctcca tacccgtttt tttggatgga gtgaaacgat
    14041 ggcgattgca attggcctcg attttggcag tgattctgtg cgagctttgg cggtggactg
    14101 cgctaccggt gaagagatcg ccaccagcgt agagtggtat ccccgttggc agaaagggca
    14161 attttgtgat gccccgaata accagttccg tcatcatccg cgtgactaca ttgagtcaat
    14221 ggaagcggca ctgaaaaccg tgcttgcaga gcttagcgtc gaacagcgcg cagctgtggt
    14281 cgggattggc gttgacagta ccggctcgac gcccgcaccg attgatgccg acggaaacgt
    14341 gctggcgctg cgcccggagt ttgccgaaaa cccgaacgcg atgttcgtat tgtggaaaga
    14401 ccacactgcg gttgaagaag cggaagagat tacccgtttg tgccacgcgc cgggcaacgt
    14461 tgactactcc cgctacattg gtggtattta ttccagcgaa tggttctggg caaaaatcct
    14521 gcatgtgact cgccaggaca gcgccgtggc gcaatctgcc gcatcgtgga ttgagctgtg
    14581 cgactgggtg ccagctctgc tttccggtac cacccgcccg caggatattc gtcgcggacg
    14641 ttgcagcgcc gggcataaat ctctgtggca cgaaagctgg ggcggcctgc cgccagccag
    14701 tttctttgat gagctggacc cgatcctcaa tcgccatttg ccttccccgc tgttcactga
    14761 cacttggact gccgatattc cggtgggcac cttatgcccg gaatgggcgc agcgtctcgg
    14821 cctgcctgaa agcgtggtga tttccggcgg cgcgtttgac tgccatatgg gcgcagttgg
    14881 cgcaggcgca cagcctaacg cactggtaaa agttatcggt acttccacct gcgacattct
    14941 gattgccgac aaacagagcg ttggcgagcg ggcagttaaa ggtatttgcg gtcaggttga
    15001 tggcagcgtg gtgcctggat ttatcggtct ggaagcaggc caatcggcgt ttggtgatat
    15061 ctacgcctgg tttggtcgcg tactcggctg gccgctggaa cagcttgccg cccagcatcc
    15121 ggaactgaaa acgcaaatca acgccagcca gaaacaactg cttccggcgc tgaccgaagc
    15181 atgggccaaa aatccgtctc tggatcacct gccggtggtg ctcgactggt ttaacggccg
    15241 ccgcacaccg aacgctaacc aacgcctgaa aggggtgatt accgatctta acctcgctac
    15301 cgacgctccg ctgctgttcg gcggtttgat tgctgccacc gcctttggcg cacgcgcaat
    15361 catggagtgc tttaccgatc aggggatcgc cgttaataac gtgatggcac tgggcggcat
    15421 cgcgcggaaa aaccaggtca ttatgcaggc ctgctgcgac gtgctgaatc gcccgctgca
    15481 aattgttgcc tctgaccagt gctgtgcgct cggtgcggcg atttttgctg ccgtcgccgc
    15541 gaaagtgcac gcagacatcc catcagctca gcaaaaaatg gccagtgcgg tagagaaaac
    15601 cctgcaaccg tgcagcgagc aggcacaacg ctttgaacag ctttatcgcc gctatcagca
    15661 atgggcgatg agcgccgaac aacactatct tccaacttcc gccccggcac aggctgccca
    15721 ggccgttgcg actctataag gacacgataa tgacgatttt tgataattat gaagtgtggt
    15781 ttgtcattgg cagccagcat ctgtatggcc cggaaaccct gcgtcaggtc acccaacatg
    15841 ccgagcacgt cgttaatgcg ctgaatacgg aagcgaaact gccctgcaaa ctggtgttga
    15901 aaccgctggg caccacgccg gatgaaatca ccgctatttg ccgcgacgcg aattacgacg
    15961 atcgttgcgc tggtctggtg gtgtggctgc acaccttctc cccggccaaa atgtggatca
    16021 acggcctgac catgctcaac aaaccgttgc tgcaattcca cacccagttc aacgcggcgc
    16081 tgccgtggga cagtatcgat atggacttta tgaacctgaa ccagactgca catggcggtc
    16141 gcgagttcgg cttcattggc gcgcgtatgc gtcagcaaca tgccgtggtt accggtcact
    16201 ggcaggataa acaagcccat gagcgtatcg gctcctggat gcgtcaggcg gtctctaaac
    16261 aggatacccg tcatctgaaa gtctgccgat ttggcgataa catgcgtgaa gtggcggtca
    16321 ccgatggcga taaagttgcc gcacagatca agttcggttt ctccgtcaat acctgggcgg
    16381 ttggcgatct ggtgcaggtg gtgaactcca tcagcgacgg cgatgttaac gcgctggtcg
    16441 atgagtacga aagctgctac accatgacgc ctgccacaca aatccacggc aaaaaacgac
    16501 agaacgtgct ggaagcggcg cgtattgagc tggggatgaa gcgtttcctg gaacaaggtg
    16561 gcttccacgc gttcaccacc acctttgaag atttgcacgg tctgaaacag cttcctggtc
    16621 tggccgtaca gcgtctgatg cagcagggtt acggctttgc gggcgaaggc gactggaaaa
    16681 ctgccgccct gcttcgcatc atgaaggtga tgtcaaccgg tctgcagggc ggcacctcct
    16741 ttatggagga ctacacctat cacttcgaga aaggtaatga cctggtgctc ggctcccata
    16801 tgctggaagt ctgcccgtcg atcgccgcag aagagaaacc gatcctcgac gttcagcatc
    16861 tcggtattgg tggtaaggac gatcctgccc gcctgatctt caatacccaa accggcccag
    16921 cgattgtcgc cagcttgatt gatctcggcg atcgttaccg tctactggtt aactgcatcg
    16981 acacggtgaa aacaccgcac tccctgccga aactgccggt ggcgaatgcg ctgtggaaag
    17041 cgcaaccgga tctgccaact gcttccgaag cgtggatcct cgctggtggc gcgcaccata
    17101 ccgtcttcag ccatgcactg aacctcaacg atatgcgcca attcgccgag atgcacgaca
    17161 ttgaaatcac ggtgattgat aacgacacac gcctgccagc gtttaaagac gcgctgcgct
    17221 ggaacgaagt gtattacggg tttcgtcgct aagtagccgc atccggtatg taacgcctga
    17281 tgcgacgctg acgcgtctta tctggcctac acgctgcgat tttgtaggcc ggataagcaa
    17341 agcgcatccg gcattcaacg cctgatgcga cgctggcgcg tcttatcagg cctacgcgct
    17401 gcgattttgt aggccggata agcaaagcgc atccggcatt caacgcctga tgcgacgctg
    17461 gcgcgtctta tcaggcctac acgctgcgat tttgtaggcc ggataagcaa agcgcatccg
    17521 gcacgaagga gtcaacatgt tagaagatct caaacgccag gtattagaag ccaacctggc
    17581 gctgccaaaa cacaacctgg tcacgctcac atggggcaac gtcagcgccg ttgatcgcga
    17641 gcgcggcgtc tttgtgatca aaccttccgg cgtcgattac agcgtcatga ccgctgacga
    17701 tatggtcgtg gttagcatcg aaaccggtga agtggttgaa ggtacgaaaa agccctcctc
    17761 cgacacgcca actcaccggc tgctctatca ggcattcccc tccattggcg gcattgtgca
    17821 tacgcactcg cgccacgcca ccatctgggc gcaggcgggt cagtcgattc cagcaaccgg
    17881 caccacccac gccgactatt tctacggcac cattccctgc acccgcaaaa tgaccgacgc
    17941 agaaatcaac ggcgaatatg agtgggaaac cggtaacgtc atcgtagaaa cctttgaaaa
    18001 acagggtatc gatgcagcgc aaatgcccgg cgttctggtc cattcccacg gcccgtttgc
    18061 atggggcaaa aatgccgaag atgcggtgca taacgccatc gtgctggaag aggtcgctta
    18121 tatggggata ttctgccgtc agttagcgcc gcagttaccg gatatgcagc aaacgctgct
    18181 ggataaacac tatctgcgta agcatggcgc gaaggcatat tacgggcagt aatgactgta
    18241 taaaaccaca gccaatcaaa cgaaaccagg ctatactcaa gcctggtttt ttgatggatt
    18301 ttcagcgtgg cgcaggcagg ttttatctta acccgacact ggcgggacac cccgcaaggg
    18361 acagaagtct ccttctggct ggcgacggac aacgggccgt tgcaggttac gcttgcaccg
    18421 caagagtccg tggcgtttat tcccgccgat caggttcccc gcgctcagca tattttgcag
    18481 ggtgaacaag gctttcgcct gacaccgctg gcgttaaagg attttcaccg ccagccggtg
    18541 tatggccttt actgtcgcgc ccatcgccaa ttgatgaatt acgaaaagcg cctgcgtgaa
    18601 ggtggcgtta ccgtctacga ggccgatgtg cgtccgccag aacgctatct gatggagcgg
    18661 tttatcacct caccggtgtg ggtcgagggt gatatgcaca atggcactat cgttaatgcc
    18721 cgtctgaaac cgcatcccga ctatcgtccg ccgctcaagt gggtttctat agatattgaa
    18781 accacccgcc acggtgagct gtactgcatc ggcctggaag gctgcgggca gcgcatcgtt
    18841 tatatgctgg ggccggagaa tggcgacgcc tcctcgcttg atttcgaact ggaatacgtc
    18901 gccagccgcc cgcagttgct ggaaaaactc aacgcctggt ttgccaacta cgatcctgat
    18961 gtgatcatcg gttggaacgt ggtgcagttc gatctgcgaa tgctgcaaaa acatgccgag
    19021 cgttaccgtc ttccgctgcg tcttgggcgc gataatagcg agctggagtg gcgcgagcac
    19081 ggctttaaaa acggcgtctt ttttgcccag gctaaaggtc ggctaattat cgacggtatc
    19141 gaggcgctga aatccgcgtt ctggaatttc tcttcattct cgctggaaac tgtcgctcag
    19201 gagctattag gcgaaggaaa atctatcgat aacccgtggg atcgaatgga cgaaattgac
    19261 cgccgtttcg ccgaagataa acctgcgctg gcaacttata acctgaaaga ttgcgagctg
    19321 gtgacgcaga tcttccacaa aactgaaatc atgccatttt tactcgaacg ggcaacggtg
    19381 aacggcctgc cggtggaccg acacggcggt tcggtggcgg catttggtca tctctatttt
    19441 ccgcgaatgc atcgcgctgg ttatgtcgcg cctaatctcg gcgaagtgcc gccgcacgcc
    19501 agccctggcg gctacgtgat ggattcacgg ccagggcttt atgattcagt gctggtgctg
    19561 gactataaaa gcctgtaccc gtcgatcatc cgcacctttc tgattgatcc cgtcgggctg
    19621 gttgaaggca tggcgcagcc tgatccagag cacagtaccg aaggttttct cgatgcctgg
    19681 ttctcacggg agaaacattg cctgccggag attgtgacta acatctggca cgggcgcgat
    19741 gaagccaaac gtcagggtaa taaaccactt tcgcaggcgc tgaagatcat tatgaatgcc
    19801 ttttatggcg tgcttggcac caccgcctgc cgctttttcg atccgcgcct ggcatcgtcg
    19861 atcaccatgc gtggtcatca gatcatgcgg caaaccaaag cattgattga agcgcagggc
    19921 tacgacgtta tctacggaga taccgactcg acttttgtct ggctaaaagg tgcacattcg
    19981 gaagaagagg cggcgaaaat cggtcgtgca ctggtgcagc acgttaacgc ctggtgggcg
    20041 gaaacgctgc aaaaacaacg gctgaccagc gcattagaac tggagtatga aactcatttc
    20101 tgccgttttc ttatgccaac cattcgcgga gctgataccg gcagtaaaaa gcgctacgcc
    20161 gggctgattc aggagggcga caagcagcgg atggtgttta aagggctgga aaccgtgcgc
    20221 accgactgga cgccgctggc ccagcagttt cagcaggagc tgtacctgcg tatcttccgc
    20281 aacgagccat atcaggagta cgtccgccaa accatcgaca aactgatggc gggtgaactg
    20341 gatgcgcggc tggtttaccg taaacgcctt cgccgtccgc tgagcgaata tcagcgtaat
    20401 gttccgcctc atgtacgcgc cgctcgcctt gccgatgaag aaaatcaaaa gcgcggtcgc
    20461 cccttgcaat atcagaatcg cggcaccatt aagtacgtat ggaccaccaa cggcccggag
    20521 ccgctggact accaacgttc accactggat tacgaacact atctgacccg ccagctacaa
    20581 cccgtggcgg agggaatact cccttttatt gaggataatt ttgctacact tatgaccggg
    20641 caacttgggc tattttgagc aaaaaaaaga gttcgccaga taccattttg atgcgtgacg
    20701 aatgctttgc catccagtac catagcgccc tttccattcc tggacctgaa taacgccact
    20761 acctcataag cacggtagcg ggtggttatt gcctgcaatt aaagatatag agccgaacac
    20821 atatgccttt tacacttggt caacgctgga tcagcgatac agaaagcgaa ttgggacttg
    20881 gaaccgttgt cgcggtggat gcgcgaactg tcactttact tttcccatct actggtgaaa
    20941 accgtctgta cgcacgcagt gattcccccg tgacccgcgt gatgttcaac cctggtgata
    21001 ccattaccag ccatgacggc tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct
    21061 tgctgaccta tatcggtact cgcctggata ctgaagagtc cggcgtagcc ctgcgtgaag
    21121 ttttccttga tagcaaactg gtgttcagca aaccgcagga ccgtctgttt gccgggcaga
    21181 ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt
    21241 tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag cctgatcccg catcagctca
    21301 acatcgctca tgatgttggt cgccgccacg cgccgcgcgt cctgctggct gacgaagtgg
    21361 gtttagggaa aaccattgaa gccgggatga tcctgcatca gcaactgctc tctggcgctg
    21421 ctgaacgtgt gctaattatc gtcccggaaa ccttacagca tcagtggctg gtagaaatgc
    21481 tgcgccgttt caacctgcgc tttgcgctat ttgatgatga gcgttatgcc gaagctcagc
    21541 acgatgctta caacccgttt gacaccgaac agctggtgat ttgctcgctg gattttgccc
    21601 gtcgtagcaa acagcgcctg gaacatctct gtgaagccga atgggacctg ctggtggtcg
    21661 atgaagcgca tcacctggtg tggagcgaag atgcgccaag ccgtgaatat caggccattg
    21721 aacaactggc agagcacgtg ccgggcgttc tgctgctgac cgcgaccccg gaacagctgg
    21781 ggatggaaag ccacttcgcc cgtctgcgtc tgctggaccc gaaccgtttc cacgattttg
    21841 cgcagttcgt tgaagagcag aaaaattatc gtccggttgc ggacgccgtt gccatgctgc
    21901 tggcaggtaa caaactgagc aatgacgaac tgaacatgct cggcgagatg atcggcgagc
    21961 aggatatcga gccgctgttg caggcagcaa acagcgacag cgaagatgcc cagagcgccc
    22021 gtcaggagct ggtttcgatg ctgatggatc gccacggcac cagccgcgtg ctgttccgta
    22081 acacgcgtaa cggtgtgaaa ggattcccga aacgcgagct gcacaccatt aagctgccgc
    22141 taccgacgca gtatcagacg gctattaaag tctccggcat tatgggcgca cgtaaaagtg
    22201 cggaagatcg tgctcgcgat atgctctacc cggagcgtat ttatcaggaa tttgaaggtg
    22261 ataacgccac ctggtggaac ttcgatccgc gcgttgagtg gctgatgggc tacctgacca
    22321 gccatcgctc tcagaaagtg ctggtgatct gcgccaaagc tgccactgcg ctgcaactgg
    22381 agcaggtact gcgcgaacgt gaaggtattc gcgctgcggt gttccacgaa ggtatgtcga
    22441 ttatcgaacg tgaccgcgct gccgcctggt ttgccgaaga agacaccggc gcacaggtac
    22501 tgctgtgctc agaaatcggt tctgaaggac gtaacttcca gttcgccagc cacatggtga
    22561 tgtttgacct gccattcaac ccggatctac tggagcagcg tattggtcgt ctggatcgta
    22621 tcggccaggc gcacgatatt cagatccatg tgccttatct ggagaaaacc gctcagtcgg
    22681 tgctggtgcg ctggtatcac gaaggtctgg atgcatttga gcacacctgc ccgaccggac
    22741 gcactattta cgatagcgta tacaacgatc tgattaacta tctggcttca ccggatcaaa
    22801 ccgaaggctt tgacgatctg atcaaaaact gccgcgagca acatgaagcg ctgaaagcac
    22861 agctggaaca gggtcgtgac cgcctgctgg aaatccactc caacggtggc gaaaaagccc
    22921 aggcactggc agaaagcatt gaagagcagg atgacgatac caacctgatc gccttcgcca
    22981 tgaacctgtt cgatattatc ggtatcaatc aggacgatcg cggcgacaac atgatcgtgc
    23041 tgacgccgtc cgatcatatg ctggtgccgg acttccctgg cctgtcggaa gatggcatca
    23101 ccatcacctt tgatcgtgaa gtggcgctgg cgcgtgaaga tgcacagttt attacctggg
    23161 agcatccgct gatccgcaac ggtctggatc tgatcctttc tggcgatacc ggtagcagca
    23221 cgatttcact gttaaaaaac aaagcgttgc cggtaggtac gctgttggtg gaactgattt
    23281 atgtggttga agcccaggct ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc
    23341 cggtacgtat gctgctggat aaaaacggca acaacctggc ggcgcaggta gagtttgaaa
    23401 cctttaaccg ccagcttaac gcggttaacc gtcacaccgg cagcaaactg gttaacgccg
    23461 tgcagcagga tgttcacgct atccttcaac tgggtgaagc gcagatcgag aaatctgccc
    23521 gtgcattgat tgatgcagcg cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc
    23581 gtctggaagc tctgcgtgca gtgaacccga acattcgtga cgacgaactg accgccattg
    23641 agagcaaccg tcagcaggta atggaaagcc tggatcaggc aggttggcgt ctggatgccc
    23701 tgcgtttgat cgttgtaacg catcagtaac ggagccgaaa atggggatgg aaaactacaa
    23761 tccaccgcag gaaccctggt tggttatcct gtatcaggat gaccatatta tggtggtcaa
    23821 caagccgagc ggtttgttgt cagtgccggg tcgtctggaa gagcacaaag acagcgtgat
    23881 gacgcgcatt cagcgtgatt atccgcaggc agaatcggtg catcgtctgg atatggctac
    23941 cagcggcgtg attgtagtgg cgctgaccaa agccgcggag cgggagttaa aacgccagtt
    24001 ccgcgagcgc gagccgaaaa agcagtatgt ggcccgcgtc tgggggcatc catcccccgc
    24061 agaaggtctg gtggatctgc cgctgatttg cgactggcca aaccgcccga aacagaaagt
    24121 ctgttacgaa acgggtaaac ctgcgcagac ggaatatgaa gtggtggagt atgcggcgga
    24181 taacacggca agagtggtgt taaaaccgat taccgggcgt tcgcatcaat tgcgtgtgca
    24241 tatgctggcg ctgggtcatc cgattctcgg cgatcgtttt tatgcatcac cagaagcgag
    24301 agcgatggca ccacgtttgt tgctacatgc agagatgttg acgattaccc atccggcgta
    24361 tggcaatagt atgacgttta aagcgccagc ggatttttaa cctttcctgt acgtttatac
    24421 cctcatccta agggctgcct cttgtacata aatccccgag tccggggatt ttttgtttct
    24481 gccaaactgc tttcagttgt tgcgtgtggc tacgctttcc attcggtaat tcccttcctg
    24541 ctccacagcg aatttttgaa ctttttcttc acccgttgac cgtctcttat atttcatttt
    24601 aacgggtctg ttatggtcct ttctgattgt tattcatggg ctaattagca gtctggttgg
    24661 cgatgcccat ctcggtgatc cgtgtcctgc acgacgcctt gcgtcactag cctcttctct
    24721 agctcatcat gcaggtcttt ctaattgtga aatcatccca cgctactgct aaggttgaag
    24781 gggcgttcca gccccttcaa ccttagctta ttctggctca ttcaataatt gcgttgtcag
    24841 taatttgttc agcagtactt ttttagtagc aacttcttca gtagcaactc ctttagtatc
    24901 aatttttttg ggggggattt tattaataat aattacctca tcatataggg aggtagcacc
    24961 gttcacggta gcaccgtcca ggatagcacc gtccgggtta gcaccgtccg gggtagcacc
    25021 gtccggggta gcaccgccca ggttagcatt ccacccaggt ctctcataaa ggatatcatt
    25081 gctacctttc attgtttttt taccaaggta acaatccctc atgtcaacgc cgggaaggta
    25141 agcctttcta agatcaacgc ccaagttacc ttggccctta tgcagctgca acgtctgagt
    25201 acatatctta ttcttttcaa tgaaaaaagt gacacttaac tcgatatccc cgaaggtctg
    25261 ggcgacccag cgcatattgc cctttgcgac caactcatcc ggattttttg cgcatgtttt
    25321 aagtttattg aataattcca ggctattgat aaacttttct ctctcattct cgttgatagc
    25381 cccagtaaag tattctgagg cgttgatgtt gccagtctgg ttattcacga aattgaacag
    25441 aagtttataa gcctcagtct gtttatctga ccagaacaga tggcgtaacg tctcccacaa
    25501 gcgttgaaag atattcatct tcacgtccat cttgtccccg ctcaccatct tataaatatc
    25561 gttcttgctc atatttaaaa gtgtaaccgg catgcctgga actgatactt tcatggtaat
    25621 agcctcgcat tgagttaaaa actcatgatg cgcgttctga ataaatatac tttcaaaaat
    25681 cgctcattca ggtatgtgtt ttttattttt aagacgtaaa aatataattc cgctcgtcgt
    25741 aaagctctca accttaagca gggtcaacgc gataaaacct acagaacccc tcccgtaaac
    25801 cgtgcattga gcctgtccac cattctgagt aaacgccttt tctcaaagaa gtgaccgcca
    25861 gggcggccac ctttaatcgt gggcagttac tcagcatcat ttacagggtc atttaaaccc
    25921 tttctgctgc tttatcagct catatgcctg ctgaatttcc tgcgctttct gcttcgccat
    25981 ctccatcatc tcaggcggca aacctttcgc caccagctta tcgggatggt gttcactcat
    26041 cagcttacgg taggcacgtt tgatggtggt cgcatcatcc gtcggcttca cgcccagcac
    26101 attacaggca tcttccagcg ttgggccacg ctgcgcttgc tgccagttac caccgccagt
    26161 ttgctgctga taaccgccgc caaactgtgc accgccctgc atcatgcgca aaaactggtc
    26221 aaactgagcg cgggagatcc ctaattcttc tgcaatgaca tacagcaccg cccgttcatt
    26281 cgggtgcagt gaaccatcag caaacgccgc ctgaatctgg atctccagaa acatacgaat
    26341 taagtcaaaa cgaccaaagc agacactgcg aaactggcgc atcttttcgc gcagcgggta
    26401 attgtctgat tttcccaccc ggaacgcatt ttgcgccgca gtacgggaag cgccatgaag
    26461 attcattcgg tccatcaact ggctggcgat atgaatatca gcctccgtga cgcgaccttt
    26521 ggatttggtt aaatgcccca tcacttcaaa agtggtggca aaaaacagcg cctgacgctc
    26581 acgctggttg gcgaaccacg ccattttacg gctacgggct ttatcaaaca tatggccaat
    26641 taacaggcct aacactacgc cccaaaagcc gccgcccatc agtaaggcca cggccacgcc
    26701 aatgattttt ccccaatact gcatatattc cccaaatcga cacacggata tcagggctat
    26761 ctcccacaat ataaaggtgc ttttaccgtt ttccggctgc ggtcaattgt gggacatcgc
    26821 ctataatttg cattatcata cctgtcattc aatgccgtgc ctaacaccac tgacgctatt
    26881 cggacaggat taacactagc gtagagatga cgagtacgtt agtctctgac cgtttgtcac
    26941 gcgcaacgtt accgatgatg gaacaataaa atcaacgtat gaaaaaacgt atccccactc
    27001 tcctggccac catgattgcc accgcccttt atagtcaaca gggactggca gccgacctcg
    27061 cctcacagtg catgttgggc gtgccaagct atgaccgtcc tctggtacag ggcgatacca
    27121 atgacttacc cgtgactatc aatgctgacc acgcgaaagg ggactacccg gatgacgccg
    27181 tgtttactgg cagcgtggat atcatgcagg gtaacagccg tctgcaggcc gacgaagtgc
    27241 agctccatca aaaagaggca ccaggacaac cggagccggt acgtaccgtt gatgcgctcg
    27301 gtaatgtcca ttacgacgat aaccaggtga tcctcaaagg gccgaaaggc tgggcgaatc
    27361 tgaacaccaa agataccaac gtctgggaag gtgattacca gatggtgggt cgccagggtc
    27421 gcggtaaagc ggacctgatg aaacaacgtg gcgaaaaccg ctataccatt ctggataacg
    27481 gtagctttac ctcctgtctg ccgggttctg acacctggag cgtggtaggt agcgaaatta
    27541 ttcatgaccg cgaagaacaa gttgcggaga tctggaacgc ccgctttaag gtgggtccgg
    27601 taccgatctt ttatagcccc tatttgcagt tgccggtggg tgacaaacgt cgctctggtt
    27661 tcttgatccc gaacgccaag tacaccacca ccaactactt tgagttctac ctgccatatt
    27721 actggaacat cgcgccaaat atggatgcca ccatcacgcc gcattatatg catcgtcgtg
    27781 gcaacatcat gtgggagaac gaattccgct acctctccca ggcgggcgct ggcttgatgg
    27841 aactggacta tctgccttca gataaagtct atgaagatga acacccgaac gatgacagtt
    27901 cacgtcgttg gttattctac tggaaccact ccggggtcat ggatcaggtg tggcgtttca
    27961 acgtcgacta caccaaggtc agcgatccta gctacttcaa tgatttcgat aacaagtacg
    28021 gttccagtac tgacggctac gcaacgcaaa aattcagcgt tggctatgcg gtgcaaaact
    28081 tcaatgccac cgtttcaacc aagcagttcc aggttttcag cgaacagaac accagtagct
    28141 actcggcaga gccgcagtta gacgttaatt actaccagaa tgatgttggt ccgtttgatg
    28201 cgcgtattta cggccaggca gtgcactttg ttaacaccag agacgacatg cctgaagcaa
    28261 cccgtgttca cctggaaccg accatcaatt tgccgctctc taataactgg ggcagcatca
    28321 ataccgaagc gaagttgctg gcaacccatt atcagcaaac caatcttgac tggtataact
    28381 ccagaaacac gaccaagctg gacgaatccg ttaaccgcgt aatgccgcaa ttcaaagttg
    28441 acggcaaaat ggtctttgaa cgcgatatgg aaatgctggc tccgggttat acccaaacgc
    28501 tggaaccgcg cgcgcagtat ttgtacgtgc cgtatcgcga tcagagcgac atctataact
    28561 acgactcgtc tctgctgcaa tctgactact ctggcctgtt ccgggaccgg acttacggcg
    28621 gtcttgaccg tattgcctcc gctaaccagg tgacgaccgg tgtcacatct cgcatatatg
    28681 atgatgctgc cgttgaacgt tttaatattt ccgttggtca aatctactat ttcacggagt
    28741 ctcgcactgg cgatgacaac ataacatggg agaatgacga caaaacgggt tcactggtgt
    28801 gggcaggcga tacttactgg cgtatctccg agcgttgggg attgcgtggc gggattcagt
    28861 acgatacacg tctggataac gtagcgacca gtaactccag cattgaatac cgtcgggatg
    28921 aagaccgtct ggtacagctg aattaccgtt acgccagccc ggaatatatt caggctacgc
    28981 tgcctaagta ctattccact gctgagcaat ataagaatgg tatttcgcag gtaggtgctg
    29041 tcgccagctg gccaattgcc gatcgttggt ccattgttgg ggcctactac tacgacacca
    29101 atgctaacaa gcaagccgac tctatgttag gtgtgcaata cagctcctgc tgctatgcaa
    29161 ttcgcgtcgg ttacgagcgg aagctgaacg gttgggataa cgataaacaa catgcggtat
    29221 atgacaacgc aatcggcttt aacatcgaac ttcgcggcct gagctccaac tacggtctgg
    29281 gtacgcaaga gatgctgcgt tcgaacattc tgccgtatca aaacactttg tgatttgttg
    29341 atttaccacg taatccgcag tgcggttaat tgaaatggaa aaagtatgaa gaactggaaa
    29401 acgctgcttc tcggtatcgc catgatcgcg aataccagtt tcgctgcccc ccaggtagtc
    29461 gataaagtcg cagccgtcgt caataacggc gtcgtgctgg aaagcgacgt tgatggatta
    29521 atgcagtcgg taaaactgaa cgctgctcag gcaaggcagc aacttcctga tgacgcgacg
    29581 ctgcgccacc aaatcatgga acgtttgatc atggatcaaa tcatcctgca gatggggcag
    29641 aaaatgggag tgaaaatctc cgatgagcag ctggatcagg cgattgctaa catcgcgaaa
    29701 cagaacaaca tgacgctgga tcagatgcgc agccgtctgg cttacgatgg actgaactac
    29761 aacacctatc gtaaccagat ccgcaaagag atgattatct ctgaagtgcg taacaacgag
    29821 gtgcgtcgtc gcatcaccat cctgccgcag gaagtcgaat ccctggcgca gcaggtgggt
    29881 aaccaaaacg acgccagcac tgagctgaac ctgagccaca tcctgatccc gctgccggaa
    29941 aacccgacct ctgatcaggt gaacgaagcg gaaagccagg cgcgcgccat tgtcgatcag
    30001 gcgcgtaacg gcgctgattt cggtaagctg gcgattgctc attctgccga ccagcaggcg
    30061 ctgaacggcg gccagatggg ctggggccgt attcaggagt tgcccgggat cttcgcccag
    30121 gcattaagca ccgcgaagaa aggcgacatt gttggcccga ttcgttccgg cgttggcttc
    30181 catattctga aagttaacga cctgcgcggc gaaagcaaaa atatctcggt gaccgaagtt
    30241 catgctcgcc atattctgct gaaaccgtcg ccgatcatga ctgacgaaca ggcccgtgtg
    30301 aaactggaac agattgctgc tgatatcaag agtggtaaaa cgacttttgc tgccgcagcg
    30361 aaagagttct ctcaggatcc aggctctgct aaccagggcg gcgatctcgg ctgggctaca
    30421 ccagatattt tcgatccggc cttccgtgac gccctgactc gcctgaacaa aggtcaaatg
    30481 agtgcaccgg ttcactcttc attcggctgg catttaatcg aactgctgga tacccgtaat
    30541 gtcgataaaa ccgacgctgc gcagaaagat cgtgcatacc gcatgctgat gaaccgtaag
    30601 ttctcggaag aagcagcaag ctggatgcag gaacaacgtg ccagcgccta cgttaaaatc
    30661 ctgagcaact aatggttaaa acccaacgtg ttgtgatcac tcccggcgag cccgccggga
    30721 gtggcccgga cttagttgtc cagcttgcac agcgtgagtg gccggtcgaa ctggttgttt
    30781 gtgccgatgc cactctcctt accaaccggg cagcgatgct cggtttgccg ctcaccctcc
    30841 gcccttattc ccccaactcc cctgcacaac cgcaaactgc gggcacatta acgctacttc
    30901 ctgtcgcgct acgtgcacct gtcactgcgg ggcagttagc ggttgaaaat gggcattatg
    30961 tggtggaaac gctggcgcga gcgtgcgatg gttgtctgaa cggcgaattt gccgcgctga
    31021 tcacaggtcc ggtgcataaa ggcgttatta acgacgctgg cattcctttt accggtcata
    31081 ccgagttttt cgaagagcgt tcgcaggcga aaaaggtggt gatgatgctg gcgaccgaag
    31141 aacttcgcgt ggcgctggca acgacgcatt taccgctgcg cgatatcgca gacgctatca
    31201 cccctgcact tttgcacgaa gtgattgcta ttttgcatca cgatttgcgg accaaatttg
    31261 gtattgccga accgcgcatt ctggtctgcg ggctgaatcc gcacgcgggc gaaggcggtc
    31321 atatgggtac ggaagagata gacaccatta ttccggtgct caatgagctg cgggcgcagg
    31381 ggatgaaact caacgggccg ctgcctgccg ataccctgtt tcagccgaaa tatcttgata
    31441 acgccgacgc cgtgctggcg atgtaccacg atcagggtct tcccgtgcta aaataccagg
    31501 gcttcgggcg cggtgtgaac attacgctgg gcctgccctt tattcgcaca tcagtggacc
    31561 acggcaccgc gcttgaactg gcgggacgtg gcaaagccga tgtcggcagt tttattacgg
    31621 cgcttaatct cgccatcaaa atgattgtta acacccaatg aataatcgag tccaccaggg
    31681 ccacttagcc cgtaaacgct tcgggcaaaa ctttctcaac gatcagttcg tgatcgacag
    31741 tattgtgtct gccattaacc cgcaaaaggg ccaggcgatg gtcgaaatcg gccccggtct
    31801 ggcggcattg accgaaccgg tcggcgaacg tctggaccag ctgacggtca tcgaacttga
    31861 ccgcgatctg gcggcacgtc tgcaaacgca tccattctta ggcccgaaac tgacgattta
    31921 tcagcaggat gcgatgacct ttaactttgg tgaactggcc gagaaaatgg gtcagccgct
    31981 gcgtgttttc ggcaacctgc cttataacat ctccacgccg ttgatgttcc atctgtttag
    32041 ctatactgat gccattgccg acatgcactt tatgttgcaa aaagaggtgg tgaatcgtct
    32101 ggttgcagga ccgaacagca aagcgtatgg tcgattaagc gtcatggcgc aatactattg
    32161 caatgtgatc ccggtactgg aagtaccgcc gtcagccttt acaccaccac ccaaagtgga
    32221 ttccgccgtc gtgcgcctgg ttcctcatgc aacgatgcct cacccggtta aagatgttcg
    32281 tgtgttgagc cgcatcacca ccgaagcctt taaccagcgt cgtaaaacca ttcgtaacag
    32341 cctcggcaac ctgtttagcg tcgaggtgtt aacgggaatg gggatcgacc cggcgatgcg
    32401 agcggaaaat atctctgtcg cgcaatattg ccagatggcg aactatctgg cggagaacgc
    32461 gcctttgcag gagagttaac gatgatcaat tcgccccgag tgtgtattca ggttcaaagc
    32521 gtctacattg aggctcaatc ttcacctgat aatgaacgtt acgtttttgc ttataccgta
    32581 accatacgca atctggggcg agcgccagtg cagttgttgg ggcgttactg gctgatcacc
    32641 aatggcaatg gccgtgaaac cgaagtccag ggcgaaggag tggttggcgt ccagccactt
    32701 atcgcgcctg gcgaagagta ccagtacacc agcggtgcaa tcattgaaac cccgctgggc
    32761 accatgcagg gtcactacga aatgatcgat gaaaatggcg tccctttcag catcgacatt
    32821 cccgtattcc gactcgccgt tcccacactc attcattaaa agaatatggc gacatacctt
    32881 attggcgacg ttcatggttg ttacgatgaa ctgatcgcat tgctgcataa agtagaattt
    32941 acccctggga aagataccct ctggctgacg ggcgatctgg tcgcgcgcgg gccgggttcg
    33001 ctggatgttc tgcgctatgt gaaatcctta ggcgacagcg tacgtctggt gctgggcaat
    33061 cacgatctgc atctgctggc ggtatttgcc gggatcagcc gcaataaacc gaaagatcgc
    33121 ctgacaccgc tgctggaagc gccggatgcc gacgagctgc ttaactggct gcggcgccag
    33181 cctctgctgc aaatcgacga agagaaaaag ctggtgatgg cccacgcagg gatcacgccg
    33241 cagtgggatc tgcagaccgc caaagagtgc gcacgcgatg tagaagcggt gctatcgagt
    33301 gactcctatc ccttctttct tgatgccatg tacggcgata tgccaaataa ctggtcaccg
    33361 gaattgcggg ggctgggaag actgcgtttt atcaccaacg cttttacccg tatgcgtttt
    33421 tgcttcccga acggtcaact ggatatgtac agcaaagaat cgccggaaga ggcccctgcc
    33481 ccactgaaac cgtggtttgc gattcctggc cctgtcgctg aagaatacag catcgccttt
    33541 ggtcactggg catcgctgga gggcaaaggt acgccggaag gtatatacgc gctggatacc
    33601 ggctgctgct ggggtggtac attaacctgc ctgcgctggg aagataaaca gtattttgtc
    33661 cagccgtcga accggcataa ggatttgggc gaagcggcgg cgtcttaaac acagcctgat
    33721 ataggaaggc cggataagac gcgaccggcg tcgcatccgg cgctagccgt aaattctata
    33781 caaaattacc gccgctccag aatctcaaag caatagctgt gagagttctg cgcatcagca
    33841 tcgtggaatt cgctgaatac cgattcccag tcatccggct cgtaatccgg gaaatgggtg
    33901 tcgccttcca cttctgcgtc gatatgcgtc agatacagtt tttgcgcttt tggcaagaac
    33961 tgttcataaa cgcgaccgcc gccaagcacc atgatttctg gtacgtcacc acacgccgcg
    34021 atggcttcat ccaccgactt cacccacgtt acgcgatcgt ccgtacccgg ttgactgctg
    34081 aggataatat ttttgcgtcc tggcaacgga cgaccgattg attcccaggt atggcggccc
    34141 ataatcacgg gtttatttaa ggtgttgcgt ttaaaccagg cgagatcggc aggcaggttc
    34201 cacggcatgg cgttttccat gccgataacg cgatctaccg ctaacgccgc aatcagactg
    34261 atcattgaga tttcccgata aaaaaaattg tcgccactat acgtaaagcg taaaccgtcg
    34321 tcgactggtg cgaggatgat gttgaggaaa attttatatt ctgctggcga gtccacgctc
    34381 tctccctgga ctcgccgcat tacaatgaaa caaaaacaaa cagttagctg taaagtgtga
    34441 tttacgtcac tctttattag gatgagggtt tcgtttccgg ttcatccgcc atgttgccgg
    34501 tatgtttacc ttcttcggtt ccctgccagc catgtcgttg aattaatgac agatgttcgc
    34561 ggtcctcggt aatgatctca cttaacatcg cgctggtgcg tttatagacc gccgcgcggg
    34621 ctttggtgtc gttctcaacc attgccatct cttccaccat ctgaatatta aagcggcgga
    34681 acacatcggc acgttctcgc gcttcatacg gccccagacc taaactttcc agtgccagac
    34741 gcccggtttt cagcgcacct tcgaaggttt cacgctccgg cttttcaacg cctgcctgac
    34801 gcaaacgaat gtagtggtcg acatcgcggg cgcgggcaat aatctgcaaa tgcgggaaat
    34861 gttctttcac catctctgtc agttgcaggt tggtttgcgg atcgtcgatg gcgttaatca
    34921 gcacttccgc tttcgccgct ccggcagatt ccagtaaatc catccgcgtg gcatcgccat
    34981 aaaacacttt cataccaaat ttacgcaagg tttcgatatg gtccggatcg tgatcgagta
    35041 ccaccatttt caccccgctg gagagcagta aacgtccggt aatctgccca aaacgaccga
    35101 atccggcgat aatcacgcgc ggctgttctt cgtcgatctc atcggcttca cgcgcttcct
    35161 cagtagaaga ttgctcaagg cgattgagga tcaccagcag aatcggcgtt gctgccatcg
    35221 acagcgccac cgccagggtc agcgatttcg cccactccgg ctccagcaca ttcgccatct
    35281 gcgccgcgcc aaataccaca aaggcaaact cactgacctg ccctaacaac accgcaaacc
    35341 aacgacgctg tttatttggc acttgcaacg gtcgggcaat cagccacagc atggcgattt
    35401 tgatgatgag gaaaccgagc agcaaaatga caatgcgcaa tgggttttca agcagcgtgc
    35461 caaagtctat cgacatgcca acaccgatga aaaacagccc caacagcaaa cctttaaatg
    35521 gttcgatatc gctctccagc gcatgacggt attccgagct tgccagcagt acgcccgcca
    35581 gaaacgcgcc catcgccatc gacaagccga cctcttccag cagcaaacca aagccaaaca
    35641 cgaggaataa cgccacggca ctaaacactt cccgcaagcc agagcgggct acaaaacgca
    35701 gcgccggacg cgtgacatag cgccccagca ataccaccag caccagcgca cccgccactt
    35761 ttaacgccga gagagcaaat gcgcccatcg tcgtcgaggc actgctcgtt gccagtagcg
    35821 gaatcatcgc caccagcggg atcgccgcga tatcctggaa cagcagcacc gcaaaggcac
    35881 tgcgacccat ttgcgtcacc atcagattgc gttcattcat cgcctgcatg gcaatcgccg
    35941 tagaggagag cgccagcgtc atgccgatca attccgcgac ctgccagcgc agcccaagta
    36001 acatgcagaa cagccccagc aggccgccgc aaatcaccat ctgcaatgcg ccaccgccga
    36061 acactgccgc acgcagcttc cacagccttt gtggatcgag ttcgaggccg ataataaaca
    36121 gcatcagcac caccccaatc tcggcaaagt gcagaataga ttcggcatcg gtcaccagtc
    36181 gcagccccca cgggccaata atgcagccgg cgatcaggta gccaagtacc gatcccagac
    36241 caagacgtac cgcaatgggt acaatcagcg ctgccgaacc gagataaatc agcgcctgaa
    36301 tcagcgtatg gctatccatg atgggcctcc tgccattcca gcggacgttg cttatagtga
    36361 cgcgcctgcc cttcgagggt ttcgtcgtca caaataaagg tgcagtgcat ggcaaacggt
    36421 ggcagccagt tcagcccgca gtagattgcc gtcgcctgta gcggctgcga cagcacatca
    36481 aagcccggat gcgcaccaat ttcaaaatgg ctttccccgc cgccggtcgt caccgcccac
    36541 agcaaatgtt tgccatgcag cgccttgccg ccgtgaccgt aagcccagcc gtgcgacaaa
    36601 actttatcga tccaaagttt aagaagcgga ggaatgctgt accactgcat cggatgctgc
    36661 cagacgatca gatcggcgcg agacagcgcc tcctgctcgg cggcaatatc gatattgaag
    36721 tcaggataga gttgataaag agagcgaatt tcgacgcctt ccagcgtcct tgcctgttca
    36781 agcatccgtt tattcgcatg ggaatgatgc ggatacggat gcgcataaat tataagaatc
    36841 ataggtagcc cgcgtgatta cgcctttgtt ttcccacaaa gtgtagtcat atattcacgc
    36901 ggcttatagt caataatatt gattcagaaa agagagaaat ctgaacaatt taccccattc
    36961 tctgcccgcg gatcgtcata ttgctggtct gcgccagtga catccctcga gtctccgggg
    37021 caaacgctac ggaaatcaac aagccaaaca gcgagatacc cgcccccatt agcatcgtgt
    37081 tactgatacc gtaattattg ataaagatcg gtagtgccca ggtcgaaaca atggtgccaa
    37141 tacgacttaa ggacataatc acgcccacgg cagaggcgcg gatatctgtc gggaagagtt
    37201 cattaggata gagccactgc aaattacccg gcccgccaga gaaaaaggca tacaccgcaa
    37261 aggccatcac taccagccag atccccatat ccgggattag ccccaaaacc gccagcgcca
    37321 gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg tccggcagtg tttaaccaca
    37381 gcatcggcgg aatacagccg agcataaaga acaggctaat caccacattc cctagtgccg
    37441 cgtttttgcc aacccccaat cccaacaaac caacgatttg cgggccaaag gtgtaaatgg
    37501 cgaacattgg gatcacctgg caggtccaga tggcggcaac aaacagaaca aaaggaaaat
    37561 ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg ctgctcttca tcgaaagcca
    37621 ccggttcgcc aaacagtttg atcatcatct cttcgcactc ttttactcgc cctttgcgta
    37681 ataaccagcg gggagattca ggcagttcga atcgaccaat caaaatcaac aaacagggga
    37741 tcgccgcgct acccagcatc cagcgccagc cgccttccac atcataaagc cagtagccga
    37801 ccagatcggc acaggtcgcg ccgacatacc acatcgcggc aataaagctg atggaaaacg
    37861 cccgctgacg ggtactggag aactcggtga tcattgaggt ggcgatggga taatctgcac
    37921 cgatgacaat gccgataagt acccgcatca ccaacagttc gacgggggat gaaacaaaca
    37981 tcgtcgccac cgatatcacg ccgatggcga tgatatcaat gaggaacatt ttgcgccgtc
    38041 cgactttatc ggaaatataa ccaaacagcg atgtgccaac gaacagcccg gcgagcgttc
    38101 ccgcgcccag caagccaatc cagtcagcgt ccagtttcag cgccggcgtc agttgctcca
    38161 gcgccacgcc aatcattacc agtacataac catccagaaa cggaccaccg cttccccaca
    38221 gcaaaatgcg gcggtgaata gaggagaatt tgagatcgtc aaagtttctg gacggttgca
    38281 tgggttattc ctgttttttg caatgagagt cagtgcggcg ttacgtatca aaccaacatc
    38341 agccgtaacg gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg
    38401 accccagccc cagaatgcga caggtgccgc actccagaca tccggcgtaa tcgaagcgca
    38461 cactgccgtc atcctgcttc ttgtacagac ctgcggggca cgctttcacc agcagctcca
    38521 gcgcctgttt atcagcatcg gccttcacaa caatgtgcgg atgctcttca tcgacattga
    38581 atttattgac gcccagtttg acgtccacat tgacgggaga agtcataaaa cggtcactcc
    38641 tttcatgcca tccttgatta gattgatgaa gcccactttc ttgccgtggc ggaggatttt
    38701 cttgcgcatc agttccggcg cgctgccatc aatggtgaac aggtcacgcg ccacacccac
    38761 cgccagctcc gggtagccgc taaacatgcg tgggttatca aggaacgccg gtagtttctg
    38821 gtacatacgc atatcgcgca gcggaccact ctcaagatgc tgacgatatt ccgccagttt
    38881 ttgcttactg aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg cggcttcccc
    38941 ggcggcaatc gccagatcca taccgcgaat ggtaaaaccg aggttcatac acattccggc
    39001 ggcatcaccg gcaatcaata cgccgtcacc aaccaactcc ggcagcatgt tgatgcctgc
    39061 ttccggcact acgtgagcgg aatattccac cagcttgccg cccgcgatca gcggtgcaac
    39121 ggccggatgc tgtttgaaat cttccagcat ttgcggcacc gattttttcg cgtcatgcag
    39181 atgatgcaaa ccacaaacca gccccagcga cagggtgttt tcattggtat aaaggaagcc
    39241 gccgcccatc aggccatcgg tgggtgatcc cgcaaacagg caagccgccc cctgattacc
    39301 ctgcaactga aaacggtctt caataaccga cttcggtaac tcgatcagtt ccttcacgcc
    39361 aaccgccaca tccgtcggtt tgacgcgttt tgccatcccc aatttttcgg caaggatgga
    39421 gttcacccca tcagcaagga tcaccgtttt cgcttcaatc acatcgccat cggcttctac
    39481 accgacgact ttgccatcgc gctgtacgag gttatcgacg cggatcccgg taattaactg
    39541 cgcgcccgct tcttcggcct gctccatcag ccaggcatca aatttactgc gcaaaacgga
    39601 gtaagaacgc tgggatggcg aggtttcgtc accattgcag tagtccatag tcatcgctga
    39661 cttttccgtc ataaacgcga gtttttcatg ggtgatcagg cgttctacgg gggcggagtc
    39721 ggcgaaacca ggaataatgt gttccagact gtgggcatag agacgcccgc cggtgacgtt
    39781 cttggcacct gcggaattgc cacgctcgat aactaacact tgcgcacctt cgcgggcgag
    39841 caccagtgcg gcaaccgaac cggcaagccc tgcaccgacg atgatggcgt caaagatatc
    39901 ttcggacata agagctccct gtcagcggca gggccaaaat gcagccctgc cagagtggat
    39961 caacgcgcta aagctgcggt cagcgccgga aggatcttca cggcgtcgcc aacaatgccg
    40021 taatccgcgt actggaagat cggcgcattt ttatctttat tgatggcgaa aatggtttgc
    40081 gacgcgttag cgccaaccat gtgctggatc tgcccggaga tccccaccgc caggtacagt
    40141 tcaggtttca gcatcaggtt ggagataccg acatagcgtt cgtgctccat ccatttttcg
    40201 ttttccgcca ccggacgaga acaggccaac tccgcaccta tcgccttgca aagctgttct
    40261 gccagcgcaa tgttctcttt gctgccaata ccgcgaccga cgctgaccac cagacgggct
    40321 ttgtcgagat cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt aatcgccaca
    40381 gccggagcct gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc
    40441 gcatcgaacg tgccgctgct gatggtcagt accgcatacg gcgtggcaat gcgttcttcg
    40501 ccaatcgcca gaccaccgta aaccatgtgt ttcactgtcg ctttaccgtc ctgtacgctg
    40561 acggtgctgg catcgttaga caccgccgct ttaaggcgat aacccagttt tgccgccagt
    40621 aatttgccgc gacgggtgtt tggcagcagc accaggccgt ctgcgccgtg ctggcgaata
    40681 gtgtcagcca tgacaccggc gtaatcttcg atcatccgat cgtccggttt gccgtttaat
    40741 ttccagacat gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc attgaggaca
    40801 aaggtgttga tttgattagc taaagcctgc gcaccgttca tcagttccgg cagacgagaa
    40861 ggggtatcgc tgaataccca gacttgagaa aacgtgttca tagcatcccc tgtaattaaa
    40921 tgactttgcg aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca
    40981 cgatgcgctg acgttcgcgc tgtttcggcg cggcaacctg ttgttctgac caggctgcct
    41041 ctgcgttaaa accaatatcc gccgccgacc atacctggac gggctttttc gccgcgccga
    41101 gaatggcttt catcgaagga atttgtgggg agttgatatc agtggaaaca gcaacaaccg
    41161 caggcagcgg aatgcttaag gtttcggttt catcttccag ttcgcgctca acggtgaggg
    41221 tatctgccgt cagggagata attttgctga cgccgttaac tgccggaata ttgaggattt
    41281 cgcccaccag cagaccaacc tgctgggcat aaaggtcgga agaaccatcg ccacagagga
    41341 tcagatcaaa gcctgctttc tgggcggctg cagccagtgc gctcgccgtt tgttgcggca
    41401 gtgcctgctc gaactggtca tcaatcacca caatcagttc atccgggccg cgcgatagca
    41461 catctttacg ccctttggcg ttggtcaggg ctttaccgcc cacacttaag gctgtcacct
    41521 gcgcctctgc tgcctgttgc tttagctggc aagccgcttc aatagcgttg agatcgtatt
    41581 ggcttatttt ggcatcggct ttgctgaagt ctaatgaacc atcagcatta ttgaccgcaa
    41641 tatcctgttc atcaggcacg cacttatagc aagtaataat cttcattgca tctccagaaa
    41701 tcatgaagga aacgtcgttc atatcggtgg tgttaattac agacatcacg ccctttaatt
    41761 ttgaaataaa actttaatca ccaatattga aaatgtcaca cgcagataaa tccaactttc
    41821 aatattgtta agttcctcac caatattgaa aacacggcgt agcaaaaaga aattttcaat
    41881 attgttttat ggatcaccaa tattgaaagt cagatgttat tttttactca caacagagca
    41941 taacaaactg attattaatc aaaattaacg aaaaaacgcc ctctattcac ccgaaacaaa
    42001 aatgtgatac caatcacaga atacagctta ttgaataccc attatgagtt agccattaac
    42061 gcgtccacga ggttaataat tatattaaat gttaacaaaa ataaaacaaa cgggaacgca
    42121 aaataaatat tcgttttcac agtggaatta actcatgaag aatgaaaaga gaaaaacggg
    42181 aatagaaccg aaggttttct ttccgccgtt aataatcgtc ggcatacttt gttggcttac
    42241 agtcagagat ctggatgcag cgaatgtcgt tattaatgct gtattcagtt acgtcaccaa
    42301 tgtatgggga tgggcatttg aatggtatat ggtggtgatg cttttcggtt ggttctggct
    42361 ggtgtttggc ccgtatgcca aaaagcgttt aggtaacgaa ccgccagaat ttagcaccgc
    42421 cagttggatc tttatgatgt tcgcctcctg tacgtctgct gccgtactgt tctggggatc
    42481 gattgagatc tactactaca tctccacccc gccgtttggc ttagaaccga actcgacagg
    42541 ggcgaaagag ttggggctgg cttacagctt gttccactgg ggacctctgc cgtgggccac
    42601 ttacagcttc ctttcagtcg ccttcgctta cttcttcttt gtccgcaaaa tggaagtgat
    42661 tcgccccagc tcgacactgg tgccgctggt aggtgaaaaa cacgccaaag ggttgttcgg
    42721 cactatcgtc gacaacttct atctcgtcgc cttgatcttc gcgatgggta ccagtctggg
    42781 ccttgccacg ccgctggtga ccgagtgtat gcaatggttg tttggcattc cgcataccct
    42841 gcaactggac gctatcatca ttacctgctg gattatcctc aacgccattt gcgtcgcttg
    42901 cggtctgcaa aaaggggtac gtatcgccag tgacgtgcgt agttacctga gcttcctgat
    42961 gctgggttgg gtgttcattg tcagcggtgc cagcttcatc atgaactact tcaccgattc
    43021 ggtggggatg ttgctgatgt atctgccgcg catgttgttc tataccgatc ccatcgctaa
    43081 aggcggcttc ccgcagggct ggaccgtgtt ctactgggca tggtgggtga tttatgctat
    43141 ccagatgagt atcttcctcg cccgcatctc ccgtggtcgt actgtgcgtg aactgtgctt
    43201 cggcatggtg ctggggctga cagcgtcaac ctggatcctg tggactgtac tcggtagtaa
    43261 cactctgctg ttgatagata aaaacatcat caacattcca aatctgatcg aacagtacgg
    43321 tgtggcgcgc gccatcattg aaacctgggc cgctctgcca ctcagcaccg ccaccatgtg
    43381 gggcttcttc atcctctgct ttattgccac cgttacgctg gttaacgcct gctcttatac
    43441 cctggcgatg tccacttgcc gcgaagtacg cgatggtgaa gaaccacctc tgctggtgcg
    43501 tatcggttgg tcaattctgg ttggcattat cggtattgtt ctgctggcgc tcggcggcct
    43561 gaaaccgatt caaaccgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacattat
    43621 ggtgacgctc tcctttatta aagacgcgaa acagaactgg aaagattaat taacccccaa
    43681 aatatcaaga ggttgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc
    43741 cggtatccgc gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg
    43801 tgacagcgtc tacccggaac gttttgtcaa agcactggcg gatatgggta tcgacagtct
    43861 gctgatccct gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg
    43921 gatggagctg ggacgtctgg gggcaccaac ctatgtgctg taccagttgc cgggcgggtt
    43981 caacaccttc ctgcgcgaag gcacacaaga gcagatcgac aaaattatgg ctttccgcgg
    44041 caccggtaag cagatgtgga actcagcgat taccgaaccg ggcgcgggct ccgacgtggg
    44101 tagcctgaaa acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg
    44161 ttttattacc agcagcgcct acaccccgta catcgtggtg atggcgcgcg acggggcttc
    44221 tccggacaaa cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg gcatcaaagt
    44281 gaccaaactt gaaaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga
    44341 cgtggaactg gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa
    44401 agaagagttc gaccatgaac gtttcctggt agccctcacc aactacggta cggcgatgtg
    44461 cgcctttgaa gatgcggcgc gctacgccaa tcagcgcgtg cagtttggcg aggctattgg
    44521 tcgtttccag ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa
    44581 aaacatgctg tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc
    44641 agcgatgtgc aaatacttct gcgccaatgc ggcatttgaa gttgtggata gcgcaatgca
    44701 ggtgctgggc ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct ggcgtgatct
    44761 gcgtgtagac cgcgtctccg ggggatctga cgaaatgcag atcctgacgc tgggtcgtgc
    44821 ggtgctgaag caataccgct aagtccgtgc gctgcctgat gcgacgctga tgcgtcttat
    44881 caggcctacg ggagcacgaa tgtaggccgg ataaagcgtt tacgccgcat ccggcagtca
    44941 tgcgctacat aaatttttca ggagcaaata atggatcatc tacccatgcc gaaattcggg
    45001 ccgttggccg gattgcgcgt tgtcttctcc ggtatcgaaa tcgccggacc gtttgccggg
    45061 caaatgttcg cagaatgggg cgcggaagtt atctggatcg agaacgtcgc ctgggccgac
    45121 accattcgcg ttcaaccgaa ctacccgcaa ctctcccgcc gcaatttgca cgcgctgtcg
    45181 ttaaatattt tcaaagatga aggccgcgaa gcgtttctga aattaatgga aaccaccgat
    45241 atcttcatcg aagccagtaa aggtccggcc tttgcccgtc gtggcattac cgatgaagta
    45301 ctgtggcagc acaacccgaa actggttatc gctcacctgt ccggttttgg tcagtacggc
    45361 accgaggagt acaccaatct tccggcctat aacactatcg cccaggcctt tagtggttac
    45421 ctgattcaga acggtgatgt tgaccagcca atgcctgcct tcccgtatac cgccgattac
    45481 ttttctggcc tgaccgccac cacggcggcg ctggcagcac tgcataaagt gcgtgaaacc
    45541 ggtaaaggcg aaagtatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac
    45601 ttcatgatgg attacttcaa cggcggcgaa atgtgcccgc gcatgagcaa aggtaaagat
    45661 ccctactacg ccggttgcgg tctgtataaa tgtgccgacg gctacatcgt gatggaactg
    45721 gtgggcatta cccaaattga agagtgcttt aaagatattg gcctcgcaca tctgcttggc
    45781 acgccagaaa tcccggaagg cactcagctt atccaccgta tcgaatgccc ttacggccca
    45841 ctggttgaag agaaactcga tgcctggctg gcgacacata ccatcgcgga agtaaaagaa
    45901 cgctttgctg aactgaatat cgcctgcgcc aaagtgctga ccgtaccgga actggaaagc
    45961 aatccacagt atgtggctcg cgaatcaatc actcagtggc aaacgatgga tggtcgcacc
    46021 tgcaaagggc cgaacatcat gccgaaattc aaaaataacc ccggacaaat ctggcgcgga
    46081 atgccctcac atggcatgga cacggctgcc attttgaaaa atatcggcta cagcgaaaac
    46141 gacattcagg agttggtcag caaaggtctg gccaaagttg aggactaaac gcgtggcgtc
    46201 agtcggcttg cctgactagc gcccgcccaa taacaaacga acaaaaatgg atagaggtgc
    46261 aatggatatc attggcggac aacatctacg tcaaatgtgg gacgatcttg cggacgttta
    46321 cggtcataaa acggcgctga tttgtgaatc cagcggcgga gtcgttaacc ggtatagtta
    46381 tcttgagtta aatcaggaga ttaaccgcac ggcaaacctg ttttatacgc tggggattcg
    46441 caaaggcgac aaggttgcac tacatctcga caactgcccg gaatttatct tttgctggtt
    46501 cgggctggca aaaattggcg cgattatggt gccgattaac gcccgcctgt tgtgcgagga
    46561 aagcgcgtgg atcctgcaaa atagccaggc gtgcctgctg gtgaccagtg cgcaattcta
    46621 tcctatgtat caacagattc agcaggaaga tgccactcaa ttgcggcaca tttgcctgac
    46681 agatgtggca cttcccgctg atgatggcgt gagttcgttt actcaactga aaaatcaaca
    46741 acctgccacc ttgtgctatg caccgccgct atcgactgac gatacggcgg aaattctctt
    46801 cacctccggc accacctccc gaccgaaagg tgtggtgatt acccattaca acctgcgctt
    46861 cgctggatat tactccgcct ggcagtgtgc actgcgtgac gatgacgtct acctgacggt
    46921 aatgcctgcg tttcatatcg attgccagtg tactgcggcg atggcggcgt tttctgccgg
    46981 ggccaccttt gtgctggtcg agaaatacag cgcccgcgcc ttctggggac aggtacagaa
    47041 gtaccgcgcc accgttaccg aatgtattcc gatgatgatc cgtacgttga tggtacagcc
    47101 gccttcagcg aacgatcagc aacaccgcct gcgggaagtg atgttttatc tcaacttgtc
    47161 ggagcaggaa aaagatgcgt tttgtgaacg cttcggcgtt cgcttgctga cgtcttatgg
    47221 gatgacggaa accattgtgg gcattatcgg cgatcgtcct ggcgataaac gacgctggcc
    47281 gtcgattggt cgggtggggt tttgctacga agcggagatc cgcgacgatc acaatcgccc
    47341 gctcccggct ggtgagatcg gtgaaatctg cattaaaggc atacctggga aaaccatctt
    47401 caaagagtac tttctcaacc cacaagccac tgcgaaagtg ctggaagccg atggctggct
    47461 gcataccggc gataccggat accgcgacga agagggcttt ttttatttcg tcgatcgccg
    47521 ctgcaatatg attaaacgtg gcggcgagaa tgtctcctgc gtggagctgg aaaatattat
    47581 cgccgcgcac ccgaaaattc aggacatcgt ggttgtgggt attaaagatt cgattcgcga
    47641 tgaagccatc aaagcatttg tggtgctgaa tgaaggtgaa acattgagcg aagaggaatt
    47701 tttccgcttc tgcgaacaaa atatggcgaa atttaaagtg ccctcttatc tggagatcag
    47761 aaaagatctg ccacgtaatt gctcggggaa aataattaga aagaatctga aataaatgaa
    47821 acagcaggga actaccctgc cagctaataa tcacactcta aaacaatatg cattttttgc
    47881 agggatgttg tcgtccctga aaaagcaaaa atggagaaaa ggaatgagtg aatcattaca
    47941 tctgacccgc aatggatcaa ttctggaaat tacccttgat cgtccaaaag cgaatgctat
    48001 tgatgcaaaa accagctttg aaatgggcga agtatttcta aatttccgtg acgatccgca
    48061 attacgtgtc gccattatta ccggtgccgg agagaagttc ttttccgcgg gctgggattt
    48121 aaaagcggca gcagaaggcg aagcaccgga tgctgacttt ggtccgggtg gttttgcggg
    48181 attaaccgaa attttcaatc tcgacaaacc ggttatcgca gctgtgaacg gctatgcctt
    48241 tggcggcggc tttgaactgg cgctggcggc agattttatt gtttgtgccg ataacgccag
    48301 cttcgccctg ccggaagcca aactgggcat cgttcctgac agcggcggtg tgctgcgtct
    48361 gccgaagatc ctgccgcctg ccatcgtcaa tgaaatggtg atgaccggca gacgaatggg
    48421 cgcagaagag gcgctgcgtt gggggatagt caaccgcgtg gttagccagg cggaactgat
    48481 ggataacgcc cgcgaactgg ctcagcagct ggttaacagc gccccgctgg cgattgcggc
    48541 gctgaaagag atctaccgca ccaccagcga aatgccggta gaagaagcgt atcgctatat
    48601 tcgcagcggc gtgttgaaac actatccatc ggttctgcat tcggaagatg ccattgaagg
    48661 gccgctggcg tttgccgaga agcgcgatcc ggtgtggaaa ggacgttaac aaccgtgagc
    48721 tattacgcct ttgaggggtt aattccggtg gttcacccga cggcgtttgt ccatcccagt
    48781 gccgtcttga ttggcgatgt gattgtggga gccggtgtct acatcggccc actcgcctca
    48841 ctgcgtggtg actacgggcg gttgatcgtg caagcgggag ccaatattca ggatggctgc
    48901 attatgcatg gctactgcga cactgacact atcgttgggg aaaacggcca tatcgggcac
    48961 ggagcgatcc tgcatggttg tttgattggt cgcgatgcat tggtcgggat gaacagcgtg
    49021 attatggatg gcgcggtcat tggcgaagag agcattgttg ccgccatgag ctttgtcaaa
    49081 gcgggctttc gcggcgagaa acgccagttg ttgatgggta cgccagcccg cgctgtacgc
    49141 aatgttagtg acgacgagtt acactggaaa cggttgaata ccaaagagta tcaggatctt
    49201 gtcgggcgct gtcatgtatc gttacatgaa acgcagccac tgaggcaaat ggaggaaaat
    49261 cgcccccgtt tgcaggggac gacggatgtg acgcctaaac ggtgatcttg ccggatgcga
    49321 cctggttgct ggataagatg ctacaacatc gcatctggca agcaggacgc ccggtatcaa
    49381 cgagataaaa ttaacgacgc atactctttg acaacatcat ctgccacttc tgctcgcggt
    49441 taagttccgt gagcggaacg gcaggaatac gcggcgtctt tcttacaccg actaattttt
    49501 cccgactgtt attgcgcagg cgcgcgtaaa tttgctcatc gatatcgacc actttaacca
    49561 gtcgctgaca ctggcatccc cgcccttcca gcttattagg gatcatctta acttcgcagc
    49621 ttatttctgt gacttccgac agaatataag tcagggtatt aaccgccttg ctgtgttcaa
    49681 tatcgaaatg aatagagatc tctcttgcta tcacccaccg attttcagcc atcatccatt
    49741 cggcgattaa caagtagagt ggtttttcaa catatccttc acacatacga actccagttt
    49801 cacaaattaa aagtctggct atgactcgtt ttgaagcaat taaacaaggc catattaaaa
    49861 ttgtggatat atcgatagta tgcaacttca ccgtcgataa gtgtgagcta aatcctgctt
    49921 atgtcatcaa aaatatcgat tccccaaaag atttacttaa tgggcaaaaa aaaacggtcc
    49981 tcatcagaga accgtattag ttattcgatc aaattagcgg atgaaaaata tctgccatga
    50041 cacgctatta tttgatctgt gcgtgcattt cctgcaccga aattactttt tcagtcgcat
    50101 cggcattcag cgccatcgcg gtggcaaagc cgccgttcag ggtggtgtcg taatgcactt
    50161 tatattgcag cgcactgcga cgaatcacgc gggagtcttc aatcgcacga cggcctgagg
    50221 tggtgttgat gatgtaggta tattcgccat tcttgatacg gtcctgaatg tgcggacggc
    50281 cttcatgcac cttgtttacc agacgcgggt tgatacctgc ttcgcccagc acaatcgccg
    50341 tgccgtgggt cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca
    50401 cgcgttcttt atcgccttcg cgcacggaaa gcagcgcacg accgtgtttc ttcatggtgg
    50461 agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa ggtgcggccc acgcccatga
    50521 cttccccggt agagcgcatt tctggcccta acagcgggtc aacgcccggg aatttattga
    50581 acggcagcac cacttctttc accgagtagt acggcgggat aacttctttg gttacgccct
    50641 gctcagccag cgatttgcca gccatcacgc gcgccgccac ttttgccagc ggtacgccgg
    50701 tggctttgga gacgaacgga acggtacgcg ccgcacgcgg gttaacttca atcaggtaga
    50761 cttcgttgtt tttcaccgca aactgcacgt tcatcaggcc gcgcacctgc aattcgaagg
    50821 ccagtttctg cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtaggctg
    50881 gcagagaaca tgcggagtca ccggagtgca cgcccgcctg ctcaatatgc tccatgatgc
    50941 cgccaatcag caccatttcg ccgtcgcaga tggcatccac gtcaacttct accgcgtcat
    51001 cgaggaagtg gtccagcaac actggcgcat cgttagacac gctgaccgcc gtctggaagt
    51061 agcgacgcag gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag
    51121 acggacgtac caccagcggg tagccaatct ctttcgcctt ctctaccgcc atttcaatag
    51181 cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg ctcaaccgca tgctggaagc
    51241 gttcacggtc ttctgcacgg tcgatagcat ccgggctggt gccgataacc ggtacgccag
    51301 cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc gccgtactgg acgataacgc
    51361 ctttcggctt ctcgatacgc acgatttcca gcacatcttc cagagttacc ggctcgaagt
    51421 agaggcggtc ggaagtgtcg tagtcggtgg agacggtttc cgggttacag ttaaccataa
    51481 tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg tacgcaacag tagtcgaatt
    51541 cgataccctg accgatacgg ttcgggccgc cgccaagcac catgattttt tcacggtcgg
    51601 tagacggatt cgcttcgcac tcttcttcat aagtggagta catgtaagcg gtgtcggtgg
    51661 cgaactctgc cgcacaggta tccacgcgct tataaaccgg gtgcaggtca tactggtcac
    51721 gcagcttacg gatttccgct tcgcgtacgc ccgccagttt tgccaagcgc gcatcggcaa
    51781 agcctttgcg tttcagctgg cgcaggaagt cagcgttcag gccagtgatg cccacttccg
    51841 ccactttctc ttccagacgc accagctctt caatctgtac caggaaccag cggtcaatgt
    51901 tggtcaggtt gaagacgccg tccacagaca ggcccgcacg gaacgcatcg gcgatgtacc
    51961 agatacgatc tgcgcctgcg tctttcagtt cgcgacggat tttggttaac gcttccgggt
    52021 catccaggct cactttcggg tcgaatccag tcgcaccgac ttccaggccg cgcagcgctt
    52081 tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc gatttcatct
    52141 gagtggtcag acggtcgtta gcaccggcga atttttcgaa gttgaagcga ggaattttag
    52201 taaccacata gtcgatggac ggctcgaagg aggccggagt acgtccgcca gtgatgtcgt
    52261 tcatcagttc gtcgagggtg taacccaccg ccagtttcgc cgccacttta gcaatcggga
    52321 aaccggtcgc tttcgacgcc agcgccgaag aacgggacac gcgtgggttc atttcgataa
    52381 caatcagacg accgtttttc gggttcaccg caaactgaac gttggaacca ccggtttcaa
    52441 cgccgatttc acgcagcacc gccatcgagg cgttacgcat gatttgatat tctttgtcgg
    52501 tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt gtggatgccc atcgcatcga
    52561 agttttcgat agagcagacg atgatgcagt tgtcgttttt atcacgcacc acttccatct
    52621 cgtactcttt ccagccgatc agcgactcat caatcagcaa ctctttggtc ggagagagat
    52681 ccagaccgcg ggcgcaaatt tcttcaaact cttcacggtt ataagcgata ccgccgccgc
    52741 taccgcccat ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca gcggcaaccg
    52801 ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc ggtttccaga ccaattttct
    52861 tcatcgctac gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg gcagtggcac
    52921 caatcatggt gacaccgaac tcttccaaca cgccctgacg ttccagctcc agcgcgcagt
    52981 tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc cgggcgctct ttttcaataa
    53041 tcttgcgtac aacttcccag tgaatcggct cgatgtaggt tgcatcagcc atttccgggt
    53101 cggtcatgat ggtcgccggg ttggagttca ccagaatgac gcggtaaccc tcttcacgca
    53161 gggctttaca cgcttgcgcg ccagagtagt caaactcaca cgcctgaccg ataacaatcg
    53221 ggcccgcacc cagaatcagg atacttttta tatctgtacg ttttggcatg gctcttttac
    53281 tcctgattac ttagcggttt tacggtactg ctcaattaac tcgataaagt ggtcgaacaa
    53341 cggcgcggcg tcgtgtggac cagggctggc ttcagggtgc ccctggaagc tgaatgccgg
    53401 tttatcggtg cgatgaatgc cctgtaacgt accgtcgaac agggatttat gcgtgacacg
    53461 caggtttgca ggtaatgttg cttcgtccac cgcaaaaccg tggttctggg cggtgatcat
    53521 taccacgttt ttctccacat ctttaaccgg atggttgccg ccgtggtgac caaatttcat
    53581 tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga tgaccgagac agatgccgaa
    53641 taccggaata tcggtttcga ggaatttctg gatggcggta atggcgtaat cgcacggggc
    53701 cgggtcgcca ggaccgttgg agaggaagat gccgtctgga ttcattttca gcacatcttc
    53761 cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct ctatccacca gcatccgcag
    53821 gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg aacggcagct cgtcttcttt
    53881 tttcgcttct ggcaggccac cggtcaacgt ccagctccct tgtgtccagc tataggcttc
    53941 tgcggtggtc acttcttttg ccagatccat gccattcaga cctgggaacg cgcgggcttt
    54001 ttctaacgcc agcgccgcat ccgggttatc gcccgcgata atgcagccat tctgtgcgcc
    54061 tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt
    54121 atggcgtttc aggtaagaag agaggtcttc ggtattacgg aagttgctgg caatcagcgg
    54181 caggtcgcga atcaccagac cttgtgcatg tacctgagaa gattcttcat cggcgtcatt
    54241 ggtgccgaca ttgccaatat ggggataagt aagagtaacg atttgacgag aataggaagg
    54301 atcagtgagg atttcttgat aaccggtcat tgaagtattg aaaacgactt ccccaaccgc
    54361 cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag
    54421 cgctgactta atcaaaacac cctccagaga atattcactc actttatttg catattaatt
    54481 cacaatgatg acgtaaatca atgcaaatct gcttaccgac gaatttctgg caaacggcgg
    54541 cattctggag atatgggcgc taaaagtcaa cttaatggcg ggatttttta tattattttg
    54601 tggctcatgc atgttttaca ggcattaagc gacaaaaaga tcatctaacc tgttctggaa
    54661 aacgcttgcg cagcaagaag attgcatgaa ataaaaacga gcagaaaaaa gtggaccaaa
    54721 tggtcaaaat ttacattaaa acaacaaaaa cagaagacaa aacatgtaat ttttggcact
    54781 tatagacaat ttaaaaacat aacaccaaaa ataaaagggc caaaaattaa agccctttat
    54841 aatcaataaa ttaaatgtgt tatttttgca ccataacaaa tattttgtgg ttacaaatta
    54901 ttgagatcaa gtacatctcg catatcaaaa agaccgcttt ccttaccact caaccacaaa
    54961 gccgatctta ccgcgccgtt agcaaatgtc atacggctgg acgccttatg ggtgatctcc
    55021 agacgctcgc caatatcggc aaacatcgcg gtatgttcac caacgatgtc acctgcacgc
    55081 acggtggcaa aaccaatggt gccaggcaca cgttcaccgg tgtggccttc acgactgtag
    55141 accgcgcaat ctttcagatc tttatcaagg gcgtgggcga tcgcctctcc cattgccagt
    55201 gcggtgcctg acggcgcatc aactttatgt ctatgatgtg cttcaataat ttcgatatcg
    55261 gtgtagtcac ccatcacttt ggctgctttc tccagcagct taagcatgac gttaacgcca
    55321 acgctaaaat tggcagcaaa gacaatcgca atatcggcag cggcgtcacg aattgcttgt
    55381 ttaccggctt cgtcaaaccc cgtagtgccg atcaccatcc ctttgccatg ctggcgacaa
    55441 aaagcgagat ggttcagcgt accttccgga cgggtaaaat cgataaacac atcaaaatca
    55501 tcttttaccg catcgaggct gctttgcacg gtaacgcctg ttttcccggc tccggccagc
    55561 tcaccggcgt cgctgcccag taaagaagat ccttcacgct ccagcgcagc gcccaactgc
    55621 acgccctcta atgccagcgc cgcctgaatc aactggcggc ccatacgccc cccggctccc
    55681 gcgatggcaa cgcggatgtt tgcatcatgc atagctattc tcttttgtta atttgcatag
    55741 accgttttca gagtaaccag cgcatcgcta cgccgccagc caaaaacgct gataattaga
    55801 aattaatgat aaacagggag ggatatcagt gaaaggcatg agtcgatgaa tgactgcatg
    55861 ccaataacat gtgacaggtt acgacgccag agccagcacc tcagccaccc actgctggaa
    55921 gcctttcaca tccagatcca atgccacctg tacattggct ggcttgccca ggcaaccgtc
    55981 gatatcaacc accgtcgtgc ctgaggtaaa ttcgccctga gtttccactg ccacaaaaca
    56041 gggtttgaga gtgaacaggt ccgggcgcac cagccaggcg atggcgcaga gatcgtgcat
    56101 tcgcaagccg ctttgcatac tgccgctacg gtagtggcta aacagggcgt gaagcatttt
    56161 cccggtacgg tttaactgcg gcagtgtaga gagatagtca ggagttaata ttgcctgatt
    56221 ggtgacatcc aaaccgcaca tgacgatttc aataccactg cggaagacac aggcagcagc
    56281 ttctggatcg gcagcaatat taaactcggc gtttggcgta cagttgccgc gtccggcaga
    56341 accacccatg atcaccagac ggcgaatata cggcttgcat tccgggcatt gtgaaagtaa
    56401 cagcgcaata ttggttaacg ggccgatggc caccagggta acaggctctg gtgcacgcat
    56461 cagggcatcc cgaatcgcca gaaacgccag tatcccgagc ggctttcggt tgtgctcaac
    56521 aaagtcgtag ccagccattc ccgattcgcc gtgcacagat gccgcatcac gcggtgcgcg
    56581 taccagtggc acagcggccc cttgggcgag cggaatctcc gcattccaga aatgcagcag
    56641 ttgcagggca ttgcgggtag ttttctcaac cgagacatta cccgcgacgg tggtcatcag
    56701 ttgcaggtcg agttcgggtg caaaaatcgc ggcggcaatg gcgacggcat cgtcaatgcc
    56761 ggggtcagta tcgaggaaga taggtaaacg catgttttct ccataaaaaa tgccggtaac
    56821 aagaccggca ttttcgcata acttaggctg ctaatgactt aatcgacttc acgaatatcg
    56881 acacgcagct ctttcggcac ttcgaaaaca atgttttctt cacggccttc cagcggaatg
    56941 gcttcaccac cgcccagctg ctgcaaacgt gccaccacat tctgcaccag aatatccgga
    57001 gccgatgcgc ccgcagtcac gccgacgcat ttaacctctt tcacccactc ttcctggatg
    57061 tctttcgcat cgtcaatcaa aaacgcgcgt ttgcccatac gctgggccag ctccgccaga
    57121 cggttggagt tggaggagtt tttcgaaccg accaccaaca caacttccgc ctgttctgcc
    57181 agggcgcgta ccgcttcctg acggttagtc gtggcgtagc agatgtcatc tttgcgcgga
    57241 ccgacaattt tcgggaagcg tttacgcagc gcgtcgatca catcagacgt gtcatccacc
    57301 gacagcgtgg tctgggtcat aaaggagagc ttctcttcgt ttttgaccgt cagtttccac
    57361 acatcgtccg gcgattcgac cagatacatt cccccttccg ggttactgta ctggcccatt
    57421 gtcccttcca cttccgggtg cccggcgtga ccgatgagaa tagattcttc gccacggcga
    57481 ctggcgcggg cgacttccat atgcactttg gtcaccagcg gacaggtggc atcaaacacc
    57541 gtcaaatcgc gactttttgc ttcgttacgt accgcctgag aaacaccgtg tgcggagaaa
    57601 atcaggatcg cgccgtccgg tacttcgcta atctgctcaa taaagatagc cccacgctca
    57661 cgcaagctat cgaccacata gcggttatgt accacttcgt gacggacata tatcggtgcg
    57721 ccgtaaatgg ccagcgcgtt ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa
    57781 ccacgcgggt tggccaacag gatctgcatg ttacgcctcc agtgccggat cgatttccag
    57841 cacttcaata tcaaaatgaa cggtctgccc ggccagcgga tggttgaaat caacggtaat
    57901 ggagtcgccg ttaatttcgc ggatcacgcc aggcatctca ctgccatcca ttgcggtaaa
    57961 aagcatgatt gcgccaattt ctggctcgcc tgcatccata aattcacggc gggagaagta
    58021 ctgaatcagg tccggtgacg gcacgccaaa cgccgcatct ggctccaacg agaaggtggt
    58081 tttatcgccc actttcagcc ccaacaggtg ttgctccagc ccttcagaaa gagaagcatc
    58141 acccaggcgg aacagcgccg gtttaccgtt gttgcgggta gactcggcgg tggtgccatc
    58201 gtcgagtttt agcgtgaagt gcaccaggac ggcgctattg ctctgtacag attcagacat
    58261 gcaggtttgc tcttatattt aaggattttt gccggatgcg gcgtaaacgc cttatccggc
    58321 ctacgatttc gcagcaatct gtaggccgga taagatgcgt cagcatcgca tccggcaggg
    58381 tttattattg ttttttcgct ctagaaggca aaaaaccttc cagcacaatc agtgccgcac
    58441 cgacacagat ggcagtatcg gcaaggttga aggtggcgaa gtgccagtcg ccgacgtaga
    58501 agtcgatcat atcgacaacg aagccgtgcc acaggcggtc gaacaggttg cccagcgcgc
    58561 cgccaataat cagcgcgtaa gcgatattgt ttagcttctg cgtggccttc gagcgataca
    58621 tcatcactgc caggatcacg ctaataccaa tcgcaatacc ggcaaagaac caacgctgcc
    58681 agccgccgct atcggcaagg aaactaaacg ccgcgccata gttacgcgca taatgcagat
    58741 taagcgacgg gaacagcggg accgtatccc ccagagcaaa gttctggagg atcaggtatt
    58801 tgctgcccag atcgataatc agcacgacta ccaccagcca cagccagcgt agccctgttg
    58861 aacagatcga ttgactcatc aggcaaactt acgtttttca ccgtcaccgg cgacgttgct
    58921 gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg ccgacatcct gggtgtagtg
    58981 ccagcagcgt gggcacttct caccttcggc tttactcaac gcgactttca gccctttgag
    59041 tacttcgctc tgctgagcat cagcaggtgc gtcgttatag tctgcaacgg tagcgccgga
    59101 ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc agtttcgccg acagttccgg
    59161 ttctgcatac aaggttactg ccgcttccag cgagccaccc actttcttgt cggcacgcgc
    59221 ttgctcaatg actttgttca cttcgccacg cactttcaac agctcgtccc agaacgcatc
    59281 gttcatcgct tcactgtctg ccaggccaaa caggccttcg taccactcac cggtgaagac
    59341 gtatttttca cgttcgcccg gcaggtagcc ccacacttca tcagcggtga aggagaggat
    59401 tggtgccatc cagcgcacca gcgcttctgc gatgtgatat agcgcagtct ggcagctacg
    59461 acgcgccaca ctgtccgctt tggcggtgta ctgacggtct ttgatgatgt cgaggtagaa
    59521 ggaacccatc tcaacggagc agaagcgcat cagacgctgt accacttcgt ggaaatcgta
    59581 tgcttcgtac gccttgagga tgtcttcctg tgccgctttc gcacaaccta cggcccagcg
    59641 atccagtacc accatctctt ccggtttcac catatctttt gctggatcaa aaccgttcag
    59701 gtttgccagc aggaagcgcg cggtgttacg gatacgacga tagctatcgg cagcacgttt
    59761 caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg gttgatgcca cccacagacg
    59821 cagaatatcc gcgcccagtt tgttcatcac atcctgcggc gaaacggtat tgccgatgga
    59881 tttagacatc ttgcggccct gaccatccac ggtaaagccg tgggtcagta cctgacgata
    59941 cggcgcttta cccttcatcg cggtggagat cattagggaa gacatgaacc agccgcggtg
    60001 ttggtcagaa ccttccagat acatgtccgc tgcgtgaccg gcaaattccg gacgcacgtc
    60061 aacaacagaa gagtgggtag atccggagtc aaaccataca tccaatgtgt ccggcacttt
    60121 cacgtactga tcagcttcgt cgccgaggat ctctttcgca tcgagatccc accacgcctg
    60181 gatgccatcg acttcaacgc gttttgccac ttcttccatc agttcaaggg tacgcggatg
    60241 cagctcttcc gtgtctttgt gcacgaacag tgacatcggt acaccccagg tgcgctgacg
    60301 ggagatacac cagtcaggac ggttagcaac catcgactcg atacgcgcct ggccccagtc
    60361 cgggatccac tgcacgcctt tgatctcttt cagtgactgc gcacgcagac ctttctgatc
    60421 catgctgacg aaccactgcg gcgtcgcgcg gaagatgatc ggcgttttgt gacgccagca
    60481 gcacggatag ctgtgctgca ttttctcaac gtgcagcagc gcgccttttt cctgcagcag
    60541 cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca tccagcgtcg gataagtgcc
    60601 cggcagataa gtgccgtccg ggccaaccgg gttagcggtt tccaggccgt atttctgacc
    60661 gatcacatag tcgtccgggc cgtggccagg cgcggtgtga acggcaccgg taccggcatc
    60721 cagggtaacg tgatcgccga ggattgccgg aacgtcgaag cccataaacg gatgggtaaa
    60781 gcgcagcagc tcaagctccg caccttttac cgtgccgaga atggtgtaat cggtcacgcc
    60841 gatacgctgc attacgcttt caaccagatc tttcgccaga atcacggcct gaccgtcgat
    60901 ctgcaccagc gcatagtcga aatctggtgc aatagagatt gcgcggttgg caggcagagt
    60961 ccacggcgtg gtggtccaga ttaccagcga gattgggccg ttaacgttgc ttacggcaaa
    61021 ttttgctttc agtgcatcct gatcgactgc ctggaaagca acgtcgatgg acggagaagt
    61081 tttgtcgtaa tactcaactt ccgcttccgc cagcgcagaa cggcagtcaa cgcaccagtg
    61141 aactggcttc gcgcctttgt gcaggtgacc gttgccgatg attttgccca gcgcgcggat
    61201 gatgttggct tcagttttga agtccatggt caggtacggg tgcgaccagt cgcccagcac
    61261 gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg gtcgccgcgt attcgcggca
    61321 cttggcgcgg aactcggcgg cggtgaattt ctcacccggc ttaccgtatt cttgctcgac
    61381 tttcagctcg atcggcagac cgtggcagtc ccagccaggc acatacggcg agtcataacc
    61441 ggaaagccct ttggacttca cgataatgtc tttcagaatc ttgttaaccg agtgaccaat
    61501 atgaatgctg ccattcgcat aaggagggcc atcatgcaga atgaaggttt ttttgccttt
    61561 tttagccgca cggatgatgc cgtacagatc atcatcagtc caacgcgcca gcattccggg
    61621 ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt tccggcaaat tcagggttga
    61681 tttatagtca ctcatcagat tctcggttcc gtatttcggt ttgattacat aacaggctta
    61741 agccggtttt gttagcccaa aaaattcgcg ggcggttaat tcatcacgcg caatctgcgc
    61801 tttcagttcg tccagcgacg caaatcgctg ctcattgcgt atttttttac gcagcactac
    61861 ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac aaatgcactt ccagctgctg
    61921 gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc acgccgggta acggcttttc
    61981 accgaggccc agcacttcta ccgcataaac ccctttcacc ggggaaacct gacggcgcag
    62041 cggtacattc gccgtcggga aacctatagt gcgccctaat tcatcaccgt ggactacacg
    62101 cccggagatg gcaaacgggt gccccagtaa actctctgcc agagccagat tgtcatccgc
    62161 aagggcctga cgcacggcgg tgctgctgat gcgcacgcca ccttcgcaaa aagtttgcgt
    62221 actggtgata tcgaagccgt attccatgcc agctttctgt aataacaaga aatcgccttc
    62281 acgaccagcg ccaaagcgga aatcatcacc tacggcaaga aattttacgc gcaaatgctt
    62341 caccagaaga tcgctgatga aattttgcgc ggttaacgcc gcgaaacgcc tgtcgaaacg
    62401 cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa cgcagttttt cccgcagccg
    62461 ggtcagtctt gccggggctt tatcggtagc aaacagttcc agtggttgag gttcaaaaag
    62521 catcaccatc accggtaagt tgcgcttgcg cccttcttcc tgcaagccct gtaacagcgc
    62581 gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc agcacacacc cttcttgcgg
    62641 ggcctggctg agattatgta tgccgcgtat cagcttcatg tctggctcaa aacagtgaaa
    62701 atcgtccgag tataccttgt acagcggtca aggttaaccg gcgattgagt accgaaatca
    62761 gaaagaagtg gcttttcatt gccatggcgc aaatcacggg aagaaactga ccgcctgctg
    62821 caatttttat cgcggaaaag ctgtattcac accccgcaag ctggtagaat cctgcgccat
    62881 cactacgtaa cgagtgccgg cacattaacg gcgcttattt gcacaaatcc attgacaaaa
    62941 gaaggctaaa agggcatatt cctcggcctt tgaattgtcc atatagaaca catttgggag
    63001 ttggaccttg gctaatatca aatcagctaa gaagcgcgcc attcagtctg aaaaggctcg
    63061 taagcacaac gcaagccgtc gctctatgat gcgtactttc atcaagaaag tatacgcagc
    63121 tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt aacgaaatgc aaccgatcgt
    63181 ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa gctgcacgtc ataaggctaa
    63241 cctgactgca cagatcaaca aactggctta atcgccaatt tgctgaagct ttgtgaaaaa
    63301 gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg atgcgtgaga gaacacactg
    63361 ccggttgcca gatagtcaaa agagacatgc cgttgtacta tctgaactct tcgtggctta
    63421 agaatcgtag aaccatcggc tgatacattc tgaacaagaa tatctactca atctccagac
    63481 tcccccacaa agaatatgga tattgtgata cacattgag
//
LOCUS       DBBGLQ010000026        57216 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.026, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000026 DBBGLQ010000000
VERSION     DBBGLQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.026"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            223..849
                     /locus_tag="ACIYSB_002012"
     CDS             223..849
                     /locus_tag="ACIYSB_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGC5429080.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            870..2972
                     /locus_tag="ACIYSB_002013"
     CDS             870..2972
                     /locus_tag="ACIYSB_002013"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGC5429081.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2985..3623
                     /locus_tag="ACIYSB_002014"
     CDS             2985..3623
                     /locus_tag="ACIYSB_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGC5429082.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYLVAEEELSVLLESIKQNGDYADIACLTGSKDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3687..4355
                     /locus_tag="ACIYSB_002015"
     CDS             3687..4355
                     /locus_tag="ACIYSB_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGC5429083.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4352..5137
                     /gene="phsC"
                     /locus_tag="ACIYSB_002016"
     CDS             4352..5137
                     /gene="phsC"
                     /locus_tag="ACIYSB_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGC5429084.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQTHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5141..5953
                     /gene="ydhT"
                     /locus_tag="ACIYSB_002017"
     CDS             5141..5953
                     /gene="ydhT"
                     /locus_tag="ACIYSB_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGC5429085.1"
                     /translation="MIITQADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5965..7569)
                     /locus_tag="ACIYSB_002018"
     CDS             complement(5965..7569)
                     /locus_tag="ACIYSB_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGC5429086.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEDSHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLLSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7695..8000)
                     /locus_tag="ACIYSB_002019"
     CDS             complement(7695..8000)
                     /locus_tag="ACIYSB_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGC5429087.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8108..8184)
                     /locus_tag="ACIYSB_002020"
     tRNA            complement(8108..8184)
                     /locus_tag="ACIYSB_002020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8148..8150),aa:Val,seq:gac)
     gene            complement(8189..8265)
                     /locus_tag="ACIYSB_002021"
     tRNA            complement(8189..8265)
                     /locus_tag="ACIYSB_002021"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8229..8231),aa:Val,seq:gac)
     gene            8573..9829
                     /locus_tag="ACIYSB_002022"
     CDS             8573..9829
                     /locus_tag="ACIYSB_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGC5429088.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITDAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9870..11243)
                     /gene="mdtK"
                     /locus_tag="ACIYSB_002023"
     CDS             complement(9870..11243)
                     /gene="mdtK"
                     /locus_tag="ACIYSB_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGC5429089.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11458..12099
                     /gene="ribE"
                     /locus_tag="ACIYSB_002024"
     CDS             11458..12099
                     /gene="ribE"
                     /locus_tag="ACIYSB_002024"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGC5429090.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12139..13287)
                     /gene="cfa"
                     /locus_tag="ACIYSB_002025"
     CDS             complement(12139..13287)
                     /gene="cfa"
                     /locus_tag="ACIYSB_002025"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGC5429091.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13578..14789)
                     /gene="punC"
                     /locus_tag="ACIYSB_002026"
     CDS             complement(13578..14789)
                     /gene="punC"
                     /locus_tag="ACIYSB_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGC5429092.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14902..15834
                     /gene="punR"
                     /locus_tag="ACIYSB_002027"
     CDS             14902..15834
                     /gene="punR"
                     /locus_tag="ACIYSB_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGC5429093.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15831..16856)
                     /gene="purR"
                     /locus_tag="ACIYSB_002028"
     CDS             complement(15831..16856)
                     /gene="purR"
                     /locus_tag="ACIYSB_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGC5429094.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17155..17244
                     /gene="cydH"
                     /locus_tag="ACIYSB_002029"
                     /gene_synonym="ynhF"
     CDS             17155..17244
                     /gene="cydH"
                     /locus_tag="ACIYSB_002029"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGC5429095.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17410..18579
                     /locus_tag="ACIYSB_002030"
     CDS             17410..18579
                     /locus_tag="ACIYSB_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5429096.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18814..19395)
                     /gene="sodB"
                     /locus_tag="ACIYSB_002031"
     CDS             complement(18814..19395)
                     /gene="sodB"
                     /locus_tag="ACIYSB_002031"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGC5429097.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19523..20338)
                     /gene="mepH"
                     /locus_tag="ACIYSB_002032"
     CDS             complement(19523..20338)
                     /gene="mepH"
                     /locus_tag="ACIYSB_002032"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGC5429098.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20672..21019
                     /gene="grxD"
                     /locus_tag="ACIYSB_002033"
     CDS             20672..21019
                     /gene="grxD"
                     /locus_tag="ACIYSB_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGC5429099.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21070..25686)
                     /locus_tag="ACIYSB_002034"
                     /pseudo
     CDS             complement(21070..25686)
                     /locus_tag="ACIYSB_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(25779..26426)
                     /gene="rnt"
                     /locus_tag="ACIYSB_002035"
     CDS             complement(25779..26426)
                     /gene="rnt"
                     /locus_tag="ACIYSB_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGC5429100.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLSAAEEV"
     gene            complement(26529..26936)
                     /gene="gloA"
                     /locus_tag="ACIYSB_002036"
     CDS             complement(26529..26936)
                     /gene="gloA"
                     /locus_tag="ACIYSB_002036"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGC5429101.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(27017..28114)
                     /gene="nemA"
                     /locus_tag="ACIYSB_002037"
     CDS             complement(27017..28114)
                     /gene="nemA"
                     /locus_tag="ACIYSB_002037"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGC5429102.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28151..28750)
                     /gene="nemR"
                     /locus_tag="ACIYSB_002038"
     CDS             complement(28151..28750)
                     /gene="nemR"
                     /locus_tag="ACIYSB_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGC5429103.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28853..29092
                     /locus_tag="ACIYSB_002039"
     CDS             28853..29092
                     /locus_tag="ACIYSB_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGC5429104.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29141..30037
                     /locus_tag="ACIYSB_002040"
     CDS             29141..30037
                     /locus_tag="ACIYSB_002040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGC5429105.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30118..30639
                     /gene="sodC"
                     /locus_tag="ACIYSB_002041"
     CDS             30118..30639
                     /gene="sodC"
                     /locus_tag="ACIYSB_002041"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGC5429106.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30640..32652)
                     /locus_tag="ACIYSB_002042"
     CDS             complement(30640..32652)
                     /locus_tag="ACIYSB_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGC5429107.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32652..33509)
                     /locus_tag="ACIYSB_002043"
     CDS             complement(32652..33509)
                     /locus_tag="ACIYSB_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGC5429108.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33512..33748)
                     /locus_tag="ACIYSB_002044"
     CDS             complement(33512..33748)
                     /locus_tag="ACIYSB_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGC5429109.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33949..34383
                     /gene="slyA"
                     /locus_tag="ACIYSB_002045"
     CDS             33949..34383
                     /gene="slyA"
                     /locus_tag="ACIYSB_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGC5429110.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34430..34897)
                     /gene="slyB"
                     /locus_tag="ACIYSB_002046"
     CDS             complement(34430..34897)
                     /gene="slyB"
                     /locus_tag="ACIYSB_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGC5429111.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35171..36280
                     /gene="anmK"
                     /locus_tag="ACIYSB_002047"
     CDS             35171..36280
                     /gene="anmK"
                     /locus_tag="ACIYSB_002047"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGC5429112.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36378..36707
                     /gene="mliC"
                     /locus_tag="ACIYSB_002048"
     CDS             36378..36707
                     /gene="mliC"
                     /locus_tag="ACIYSB_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGC5429113.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36766..37422
                     /gene="pdxH"
                     /locus_tag="ACIYSB_002049"
     CDS             36766..37422
                     /gene="pdxH"
                     /locus_tag="ACIYSB_002049"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGC5429114.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37551..38825
                     /gene="tyrS"
                     /locus_tag="ACIYSB_002050"
     CDS             37551..38825
                     /gene="tyrS"
                     /locus_tag="ACIYSB_002050"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGC5429115.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38884..39747
                     /gene="pdxY"
                     /locus_tag="ACIYSB_002051"
     CDS             38884..39747
                     /gene="pdxY"
                     /locus_tag="ACIYSB_002051"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HGC5429116.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39791..40396)
                     /gene="gstA"
                     /locus_tag="ACIYSB_002052"
     CDS             complement(39791..40396)
                     /gene="gstA"
                     /locus_tag="ACIYSB_002052"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGC5429117.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40502..42004)
                     /gene="dtpA"
                     /locus_tag="ACIYSB_002053"
     CDS             complement(40502..42004)
                     /gene="dtpA"
                     /locus_tag="ACIYSB_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGC5429118.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42615..43250)
                     /gene="nth"
                     /locus_tag="ACIYSB_002054"
     CDS             complement(42615..43250)
                     /gene="nth"
                     /locus_tag="ACIYSB_002054"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGC5429119.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43250..43945)
                     /gene="rsxE"
                     /locus_tag="ACIYSB_002055"
     CDS             complement(43250..43945)
                     /gene="rsxE"
                     /locus_tag="ACIYSB_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGC5429120.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(43949..44569)
                     /gene="rsxG"
                     /locus_tag="ACIYSB_002056"
     CDS             complement(43949..44569)
                     /gene="rsxG"
                     /locus_tag="ACIYSB_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGC5429121.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44573..45631)
                     /gene="rsxD"
                     /locus_tag="ACIYSB_002057"
     CDS             complement(44573..45631)
                     /gene="rsxD"
                     /locus_tag="ACIYSB_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGC5429122.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCAMLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45632..47854)
                     /gene="rsxC"
                     /locus_tag="ACIYSB_002058"
     CDS             complement(45632..47854)
                     /gene="rsxC"
                     /locus_tag="ACIYSB_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGC5429123.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIATALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKREQQPANAEPEEQVDP
                     RKAAVEATIARAKARKLEQQQANAVPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(47847..48425)
                     /gene="rsxB"
                     /locus_tag="ACIYSB_002059"
     CDS             complement(47847..48425)
                     /gene="rsxB"
                     /locus_tag="ACIYSB_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGC5429124.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48425..49006)
                     /gene="rsxA"
                     /locus_tag="ACIYSB_002060"
     CDS             complement(48425..49006)
                     /gene="rsxA"
                     /locus_tag="ACIYSB_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGC5429125.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(49083..49523)
                     /locus_tag="ACIYSB_002061"
     CDS             complement(49083..49523)
                     /locus_tag="ACIYSB_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGC5429126.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIALWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49609..49824)
                     /gene="ydgT"
                     /locus_tag="ACIYSB_002062"
     CDS             complement(49609..49824)
                     /gene="ydgT"
                     /locus_tag="ACIYSB_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGC5429127.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(50097..50222)
                     /gene="blr"
                     /locus_tag="ACIYSB_002063"
     CDS             complement(50097..50222)
                     /gene="blr"
                     /locus_tag="ACIYSB_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGC5429128.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            50465..51505
                     /locus_tag="ACIYSB_002064"
     CDS             50465..51505
                     /locus_tag="ACIYSB_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGC5429129.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51539..52540)
                     /gene="add"
                     /locus_tag="ACIYSB_002065"
     CDS             complement(51539..52540)
                     /gene="add"
                     /locus_tag="ACIYSB_002065"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGC5429130.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52644..53816)
                     /gene="malY"
                     /locus_tag="ACIYSB_002066"
     CDS             complement(52644..53816)
                     /gene="malY"
                     /locus_tag="ACIYSB_002066"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGC5429131.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53826..55418)
                     /gene="malX"
                     /locus_tag="ACIYSB_002067"
     CDS             complement(53826..55418)
                     /gene="malX"
                     /locus_tag="ACIYSB_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGC5429132.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     IGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55593..56621
                     /gene="malI"
                     /locus_tag="ACIYSB_002068"
     CDS             55593..56621
                     /gene="malI"
                     /locus_tag="ACIYSB_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGC5429133.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56733..>57216
                     /locus_tag="ACIYSB_002069"
     CDS             56733..>57216
                     /locus_tag="ACIYSB_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGC5429134.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     S"
BASE COUNT        14441 a        14217 c        14897 g        13661 t
ORIGIN      
        1 aattttaatt gataacgatc aatgttaagg gctcagaata atcacattat tttttctcct
       61 aaccataaag gattagttta tcggcgttaa tcttttctca tacactatcg ggttagggaa
      121 aatatctggt gttggggaca gagtcagcag cgtattagtt gaatgcttaa tatcaggctg
      181 ctgaattgtc agcactgtgt ttgcgaagag gatgcgaaat aaatgaaccc ggttgatcgt
      241 ccactattag atattggctt aacgcgatta gaattcttgc gtatatcagg aaaaggcctt
      301 gctggtctaa caattgcccc tgcgttactg tcacttttgg gttgcaaaca ggaagatatt
      361 gatagcggca cagtagggtt gataaatacc cccaaagggg tgctggtgac ccagcgagcg
      421 cgatgcacgg gttgccaccg ctgtgaaatc tcttgtacca acttcaacga tggctcagta
      481 gggacattct tctcccgtat caaaatccat cgcaattatt tctttggcga caacggggtt
      541 ggctctggcg gcggcctgta tggcgatctc aactataccg cggacacctg ccgtcaatgc
      601 aaagagccgc aatgcatgaa cgtctgcccg attggtgcga ttacctggca gcagaaagaa
      661 ggctgtatta ccgtcgatca taaacgctgt attggctgta gcgcctgtac tacggcatgc
      721 ccgtggatga tggccaccgt aaataccgaa agtaaaaaat cctcgaaatg tgtgttatgc
      781 ggtgaatgcg caaacgcctg cccaacaggg gcgttaaaaa ttatcgagtg gaaagatatt
      841 actgtgtgaa tcttgcaaag gaaaaaatca tggctaacgg ttggacaggt aatatattaa
      901 gagtcaatct cacgacagga aatattaccc tcgaagattc cagtaagttt aaaagttttg
      961 tcggtggcat gggcttcggc tacaaaatta tgtatgacga agtaccgcca ggcacgaaac
     1021 ctttcgatga agcgaataaa ttagtctttg ctaccggccc attaactgga tctggtgccc
     1081 cctgtagttc tcgcgtaaat atcacctcac tttctacttt taccaaagga aatttagtcg
     1141 tcgatgccca tatgggtggc ttttttgcag cgcaaatgaa attcgctgga tacgacgtca
     1201 ttattatcga agggaaggcg aaatcaccgg tatggctgaa gattaaagat gacaaagtta
     1261 gcctggaaaa agccgatttc ttatggggaa aagggacgcg cgcaacgacg gaagaaattt
     1321 gtcgattgac cagtccggaa acctgtgtgg cggctattgg tcaggctggg gaaaaccttg
     1381 ttcctctctc tggcatgttg aatagccgta accacagcgg cggtgcggga actggcgcaa
     1441 taatgggttc gaaaaacctg aaagcgattg cggttgaagg gacgaaaggg gtcaacattg
     1501 ccgatcgtca ggagatgaag cgtctcaatg attacatgat gactgaactt attggtgcga
     1561 ataacaacca tgtcgtgcca agtacgccac aatcgtgggc agagtattca gatcccaagt
     1621 cacgctggac agcacgtaaa gggctgtttt ggggcgcggc tgaaggtggt ccgattgaaa
     1681 cgggtgaaat tccgccaggc aatcagaata cggtcggctt tcgtacctat aaatccgttt
     1741 ttgacttagg accggcggca gagaaataca cagtaaaaat gagcggctgc cactcttgcc
     1801 cgatccgttg tatgacccaa atgaatattc ctcgggtgaa agagtttggc gtgcccagca
     1861 caggtggtaa cacttgtgta gcaaactttg tccataccac catcttcccg aacgggccga
     1921 aagattttga agataaagac gatggtcgtg tgattggtaa cctggtgggt ctgaatttgt
     1981 tcgatgacta cggcctatgg tgtaactacg ggcagttgca tcgcgacttt acttattgtt
     2041 acagcaaagg tgtgttcaag cgtgttctgc cagctgaaga gtatgcagaa attcgctggg
     2101 atcaactgga agcgggtgac gttaacttca ttaaagattt ttactaccgt ctggcgcatc
     2161 gtgtgggtga gctgagtcac ctggctgatg gttcatatgc catcgcagaa cgctggaatt
     2221 tgggtgaaga gtactggggc tacgcgaaaa ataaactctg gtcgccgttt ggctatccgg
     2281 ttcaccatgc caatgaagcg tcagcgcagg tcggttccat tgttaactgt atgttcaacc
     2341 gtgactgcat gacgcatacc catatcaact ttattggttc cggcttgcca ttgaaactgc
     2401 aacgtgaagt ggcgaaagaa ctttttggtt ctgaagatgc ttacgatgaa accaaaaact
     2461 acacgccaat caatgacgca aaaattaaat atgcaaagtg gtcgctgttg cgggtctgtt
     2521 tgcataacgc cgtcaccctg tgcaactggg tctggccaat gaccgtttcg ccgctgaaaa
     2581 gccgtaatta ccggggcgat ctggcgcttg aagccaaatt cttcaaagcg atcaccggcg
     2641 aagagatgac tcaggaaaaa ttagatttag ctgcagagcg tatttttacg ttgcatcgtg
     2701 cctacacggt aaaactgatg caaaccaaag atatgcgtaa tgaacacgat cttatctgtt
     2761 cctgggtatt cgacaaggat ccgcagatcc cggtctttac tgaaggtact gacaaaatgg
     2821 atcgtgacga tatgcatgct tcgctcacga tgttctacaa agaaatgggc tgggacccac
     2881 agcttggttg tccaacccgc gaaacattgc agcgtctggg gctggaagat attgccgccg
     2941 atctggcagc acacaatcta ctgcctgcgt aaggaatggg taagatgaac catcaggatg
     3001 aattgccgtt agcgaaagtc agcgaagttg acgaggcgaa gcggcaatgg ttgcagggga
     3061 tgcgtcaccc cgttgatacg gtaaccgaac cagaacctgc tgaaatcctg gctgagttca
     3121 ttcgccagca ttctgcggct gggcagctgg tggcgcgcgc cgtgtttctc tcgccaccct
     3181 atttggttgc agaagaggaa ctgtcagttt tgctggaaag cattaagcag aacggagact
     3241 acgcggatat cgcttgtttg acgggaagca aggatgatta ctattactca acgcaggcca
     3301 tgagtgaaaa ctacgcggcg atgtctttgc aagttgtgga gcaggacatt tgtcgcgcca
     3361 ttgcacacgc cgttcgtttt gagtgccaga cgtatcctcg tccttacaaa gtcgccatgc
     3421 tgatgcaagc gccatattac ttccaggaag cgcagattga agccgcgatc gccgcgatgg
     3481 atgttgcacc tgaatatgcc gatatacggc aggtggagtc atcgacggcg gtgttgtact
     3541 tgtttagcga gcgatttatg acctatggca aggcttatgg cttatgcgaa tggttcgagg
     3601 tagagcagtt tcagaacccc tgaaatttca gggagatgtc atggataggg tggacagttg
     3661 cagccactgc attaggggat aatcagatgt ccttcactcg acgcaaattt gttctgggga
     3721 tgggaacagt aatctttttt actggttctg cgtcttctct gttagcgaac acgaggcaag
     3781 aaaaggaagt tcggtatgcc atgattcatg acgagtcacg gtgcaatggc tgtaatattt
     3841 gcgcccgcgc atgccgtaaa actaaccacg tccccgctca gggaagccgt ttatcgatag
     3901 cacacattcc agtcaccgat aacgacaacg aaacgcagta tcactttttt cgccagtcat
     3961 gtcagcactg cgaagatgct ccgtgcatcg acgtttgccc gacgggggcg tcgtggcgtg
     4021 atgagcaggg gatcgtgcgg gtagaaaaat cgcagtgcat cggctgcagt tattgcattg
     4081 gcgcttgtcc gtaccaggtt cgctacctta accctgtcac taaagtggca gataaatgtg
     4141 atttctgcgc tgagtcccga ctggcaaaag gctttccgcc catttgcgtc agcgcctgcc
     4201 cggaacacgc attgatcttt ggtcgcgaag atagcccaga aattcaggcg tggttgcagc
     4261 aaaataaata ctatcaatat cagctacctg gtgccgggaa accgcatctg tatcgccggt
     4321 tcggtcaaca tttgattaaa aaggaaaatg tatgaacccg tcgcaacatg ctgaacagtt
     4381 tcagagccag ttagcgaact atgtgccaca gttcactccc gaattttggc cggtgtggtt
     4441 gatcattgcc ggagtattgc tggttgggat gtggttggtg ctggggctgc atgccttgct
     4501 tcgtgctcgt ggcgtgaaga aatcagccac cgatcatggt gagaagattt atctttacag
     4561 caaagcggtc agattatggc actggtcgaa tgcgttactc tttgtattgt tgctggccag
     4621 tgggctgata aatcactttg cgatggtggg cgcaactgcg gttaaaagtc tggttgcggt
     4681 gcatgaagtt tgcggatttt tgttactggc atgctggctc ggctttgtgc tgatcaatgc
     4741 cgttggggat aatggtcacc actatcgcat tcgtcgtcag gggtggctgg aacgagcggc
     4801 aaaacaaacg cgattttatt tgtttggcat tatgcagggg gaagaacatc ctttcccggc
     4861 aacaacccag tctaaattta atcccttaca gcaggtcgcc tatgttggtg tcatgtatgg
     4921 attgctgccg ttgttactat tgacggggct gctgtgtctc tatccgcaag ccgtgggaga
     4981 tgtgtttcct ggcgtaagat actggttatt gcagacacat tttgctctgg catttataag
     5041 cctctttttt atcttcggtc atctttatct ttgcaccacg gggcgtacgc cacacgaaac
     5101 ctttaaaagc atggtcgatg gctatcaccg gcactaacga atgatcatta cacaagcaga
     5161 cctgcgagaa tggcgtatcg gagcggtgat gtatcgctgg ttcctgcgcc atttcccacg
     5221 cggcggtagt tatgccgata ttcatcatgc actgattgag gaaggatata cggactgggc
     5281 ggagagtctg gttgagtatg cgtggaaaaa atggctggcg gatgaaaact tcgcgcatca
     5341 ggaagtttcc tctatgcaaa aactggccac agaccccgga gagagaccat tttgcagtca
     5401 gtttgcccgc agcgacgatc atgctcgcat cggctgttgt gaggataacg ccagaatcgc
     5461 aaccgctggg tatgccgcgc aaattgccag tatgggttat agcgtgcgga ttggaagtgt
     5521 cggctttaac agtcacattg gcagctccgg tgaacgcgca cgggttgctg taaccggtaa
     5581 ttcttcgcgg ataagcagcg ctggagatag cagtcggatt gcgaataccg gaatgcgagt
     5641 acgcgtttgt acattgggtg aacggtgtca tgtcgccagt aatggtgacc tggtgcaaat
     5701 cgccagcttt ggcgcaaacg ccagaattgc caacagcggc gacaatgtac acatcattgc
     5761 cagcggtgaa aattcaacgg tagtgagcac gggcgtggtc gactcaatta ttctcgggcc
     5821 aggaggaagc gccgcactgg catatcatga cggcgaaaga gttcgcttcg ccgtcgccat
     5881 tgaaggtgaa aacaatattc gagccggagt ccgctatcgt cttaatgaac agcatcagtt
     5941 tgttgagtgc tgatagcttt acctttaatc aaacgaaaga cgccgacgag cacgggatgc
     6001 aggctttacg accgcccgag ccatcgcctc accaatttct gcacatgccg taagtccctg
     6061 aacgaaaggc tggtcgtgca tcaaccaggg caacgcgccg aacgctacgc gcccacgaat
     6121 atcttcgggt tgctgtaacg tataatcttc gccaacatca gggatttcat cccctgtttt
     6181 ctgtaattgc tcgcgtagcc cagggaaagg gatatctttc actttaagcg gacgttgtcc
     6241 gcgggcatca ataaaaacgt caaacgagta gctgttgtct tccgttttca ggacggtgcg
     6301 cgactcatta atttccattt tgtagtcttc accgagggcg agaatatgaa tgattcccgc
     6361 ttcacgtaag gcaagtaggc gacgaataga ctctgaaggg attgccgcat aattatcgat
     6421 gaaaacccgg gcaaggcctt tactgaaccg tttatggtcg tgttcattca gatgtggaac
     6481 aatttcctgt acggcttcat gcaggcgcag aatgacataa cgccagggaa cagtatgttt
     6541 ttctcgttta ttgcgttcga cttcctggag atttttttct gcccagtcaa atgggtcgcg
     6601 ttgtttgcgc tcggcaaacc aggcttgagc aaaggaatcg acattcaggc tctctaaggc
     6661 tatgcgttga ctccagtctg gatcagcaaa cttgatttcc tctactatca atctaaatac
     6721 ccgatccaaa aggccctctt cgcctttttg aatctcagca tttaatgcct gatcggtgac
     6781 gatgtgtaag ggctcgtagg gaatagggca atagaaatcg gcttcgggta aaatacccgt
     6841 gcgcgacaac aacgtgatat ttagcttttc acttgcgtta tcgcggtgaa aaacgacgtg
     6901 ttgtttatca tcttcaatga acgaaccatg ctgaatagcc actgccattg ccgcatccag
     6961 tccgctcaag gatgttccca taatacccac gttacacgca tcgacctttg cttccatcag
     7021 gcctgaccac gggctgggaa aatacgttcg ggttgcttct tcttcatcag gccagacgtg
     7081 acccgtggcg atcaccgcta aatcaaacgt ctcgctgggt aaatcctgat ttgtagcgag
     7141 catgacgcca gcatttgtaa tttgcagatc ggtaacctgg catgattcat aaacagccac
     7201 tgcaaatttt tgctgtcgtg cctggtctac tagtcgtaaa aattgatcgc ggaaatattc
     7261 gcccagcaga attcgcggta aaaactgacg atcgtgcaag gtttcttttt taacgccata
     7321 acgctggaga tggctgtctt cttgcttttg tagccattcg agatacgtac aataaatcgg
     7381 cggtatttca atactggcaa tatttgccag catcattttt gagttttcct catcactgta
     7441 tggcatcccg acaccggcct cgtcagcctg ctcgaagata gaaatagaaa gtggagtttg
     7501 ttgctgtaga agcgagaata aggtgtagat ccccgtaggc ccggcaccca caatagcaat
     7561 ttttttcatt gttacttacc tgtacgcatt ctttcgaatt tagtgttatc agataagttt
     7621 ggttgagatt tttagaattt gagaaatttc agacagggat gaaaagggcg ctaaccgccc
     7681 ttgattaaat tctgtcaggc gagttttgcc tgattgattt gactaagtgg ctcattgaca
     7741 tcgaaaactt tggcgacaac ttcctcaacg ccgagatttt tcagtcgggc agtatgtttt
     7801 tccagatagg caagggcgct tttttcatca gtgaaaaggt agatcccacc ggcttcgtgg
     7861 ttcttttcac tttctgtcca taccttccac agaaaaccag gttcctgatt aatcgactca
     7921 gcaagtggtt taagctgctc agccattgcg tcgccaaatg ggccattaaa agcaaaatga
     7981 agttgtaaca aagttgccat agcgattctc cttctgataa cgcatacgaa gacagtgtaa
     8041 atcttttaac gtattggata cagagaagaa acagagggaa aaaggagaag accagattaa
     8101 gaaaatctgg tgcgtccgag tggactcgaa ccaccgaccc ccaccatgtc aaggtggtgc
     8161 tctaaccaac tgagctacgg acgcaggatg gtgcgttcaa ttggactcga accaacgacc
     8221 cccaccatgt caaggtggtg ctctaaccaa ctgagctatg aacgcaacgt tgtaggtgac
     8281 aacggggacg aatattagcg gcagagtggg aaggtggcaa gaggcaaaac gtaattttct
     8341 gcgctatttc gaccgtttgc agagctttta agcaaattgg ctatattttg ttgatttgca
     8401 agggtggttt ttattcagga tcgcatttac atctgataca acccaataat tcgtgtatca
     8461 gagtttaaac agagttgttt gccaccatct caaatctgtc gtggataaat cctccgtagc
     8521 ctttacactt aggtagcaat aaaggcacac agatgcagca aaggaataaa tcatgggatc
     8581 tgatgcgaaa aacttgatga gcgacgggaa cgtgcaaatt gttaagaccg gcgaggtcat
     8641 tggcgcgacg caacttactg aaggcgagtt gattgttgaa gctggcggaa gagccgaaaa
     8701 taccgtggtc acgggggctg gctggttgaa agtggcaacc ggtgggatcg ccaaatgcac
     8761 acagtacggt aacaatggca cgctatcggt cagcgatggt gccattgcca cagatattgt
     8821 tcagtccgag ggaggcgcaa ttagtctctc tacgctcgct acggttaatg gccgccatcc
     8881 cgaaggtgaa ttcagcgttg ataaaggtta tgcctgcggt ttgttgctgg aaaatggcgg
     8941 taacctgcgt gtactggaag gacatcgcgc ggaaaaaatc attctcgatc aagagggcgg
     9001 cctgttggtt aatgggacaa cctcagcggt cgtggtagat gaaggtggtg aattgttggt
     9061 gtatccaggt ggggaagcca gcaattgtga gattaatcag ggcggcgttt ttatgctggc
     9121 cgggaaagcc agtgatacgt tgcttgctgg tggcaccatg aataatctcg gtggtgaaga
     9181 ctctgacact attgttgaga atggatccat ttatcgtctg gggacggatg gccttcagct
     9241 ctacagttcc ggtaagacgc aaaacctgtc cgtgaatgtg ggtggtcggg ctgaagtgca
     9301 tgccggtacg ctggaaaacg cggtaataca aggtgggaca gtgatcctgt tgtcacccac
     9361 cagcgcggac gaaaattttg tcgtagagga agatcgcgca ccggttgaac tgaccgggag
     9421 tgttgcatta ctggacggcg cttcaatgat tattggttat ggcgcagagc tgcaacaatc
     9481 aacgattact gtacagcagg gcggtgtgtt gattctagac ggcagtacgg taaaaggtga
     9541 cagtgtcact ttcattgttg gtaacatcaa tctgaatggt ggaaaactgt ggctgatcac
     9601 tgatgcggca acgcatgtgc aactgaaagt gaaacgcctg cgcggagagg gagcgatttg
     9661 cctgcaaacc agtgcaaaag aaatttcacc tgacttcatc aatgtgaaag gggaagttac
     9721 cggggatata cacgttgaga taacagatgc cagtcggcaa actctgtgta acgctctgaa
     9781 attacagcca gacgaagacg ggattggcgc aacgctccag cctgcgtaaa aatacaggcg
     9841 caacccgtga aggttgcgcc ttgtctttat tagcgggatg ctcgttgcag aatgatggct
     9901 gacggcagac gttgcaggaa ccgcatacgc aacatcatca taatggctgc cgacgtcagg
     9961 ccaataataa agcctatcca gaagcctgct ggccccatag gttcaacgac cagatcggtc
    10021 agtgccagaa tatagccgct tggcaagccc agcacccagt aagccgtaaa ggtaatatag
    10081 aaaatggaac gcgtatcttt ataaccacgc aaaatcccac tgccaatcac ctggattgag
    10141 tcagaaatct gatataccgc cgccagcaac atcaaatgcg cagccagcgt tacaacctcg
    10201 ggattgtcgt tgtacaacag ggcgatttgc tcccgcagtg aaaccgtgaa aatggccgtc
    10261 agggttgcca tacagacacc caccataagc ccggtccgcg cagcggtttg cgcatccagc
    10321 gttgagccct gacccagacg ataacctacg cggatagtta ccgctgccgc cagcgacatt
    10381 ggaagcacga acattagtga actaaagttc agggcaatct ggtgtcctgc gacatcaaca
    10441 ataccgagcg gagacactaa cagagccacg acggcaaaca gtgtcacttc aaagaacagt
    10501 gccagcgcaa tcggcaaacc gagttgaatc agtcgtttca taaccgcagg atcgggtttt
    10561 gcggtgcctt tttcgttacg aatatcgcgc atggagcggg cgcgtttaat gtaagaaacc
    10621 atggcaagga acatgaccca atacaccgcc gcagtagcca cgccacaacc aacgccaccg
    10681 agctcaggca taccgaaatg accataaata aagatatagt tcaccgggat gttcaccagc
    10741 aggccgataa agcccattac catacccggc ttggtttttg ccagaccttc acactggtta
    10801 cgggcaacct ggaagaacag atatcccggc gcgccccaca acaacgcacg cagataaccc
    10861 acggctttgt ccgccagagc cggatcgatg ttttccatgg agcggataat gtaacctgca
    10921 ttccacagca ccagcataat gagaacggaa acaaaacctg ccagccagaa accttgtcgc
    10981 acctgatgcg caatgcgctc acgtcgaccg gaaccattta attgcgcgat aaccggcgtt
    11041 aatgccagca gcagtccgtg accaaagagg atcgccggaa gccagataga agtaccgata
    11101 gcgaccgccg ccatgtcggt ggcactatag ccgcccgcca tcacggtatc gacaaaaccc
    11161 atcgcagttt gggcgatttg cgcgagaatc accgggattg ctaatgctaa taacagacgc
    11221 gcttcactga tatacttctg cacgtgaaca ccttttattt gtagttatat gagagactaa
    11281 aaagccgcct gaatgggcag caaaaagaag aagcagggga aattcagtct attgtagcga
    11341 ggatttatta attctccagt gaaaaaatag ccattcttac cccttcactg gtaagggcgt
    11401 tttccacctg ctattgtgct gaacagaatg tcttaactga tttcaggagt tgtaagtatg
    11461 tttacgggga ttgtacaggg caccgcaaaa ctggtgtcga ttgacgagaa accaaatttt
    11521 cgtacgcatg tggtggagtt acccgaccac atgctggacg gcctggaaac cggtgcttcc
    11581 gtggcgcata acggttgctg cctgaccgtg acggaaatta acggcaacca tgtcagtttt
    11641 gacctgatga aagaaacgtt acgcattacc aatcttggcg atttaaaagt gggggattgg
    11701 gtaaacgttg agcgtgcggc gaaattcagt gatgaaattg gcggacactt aatgtcaggt
    11761 catattatga ccactgctga agtggcgaaa atattaacct cagaaaataa tcgccagatc
    11821 tggtttaaag tccaggatag tcagttgatg aaatatattc tgtacaaagg atttattggc
    11881 atcgacggta ttagcctgac cgtcggcgaa gtcacgccaa cgcgtttttg cgtccattta
    11941 attccggaaa cactggaacg cacgactctt gggaagaaaa aacttggcgc acgcgtcaac
    12001 attgaaatcg atccacaaac tcaggcagtg gtagatacgg tagaacgtgt gctggcggca
    12061 cgagaaaatg ccatgaatca accaggcaca gaagcctgat ctgaaatcgc ccccggagag
    12121 ggggcgatag aatagccttt agcgagccac tcgaaggccg ttttcaacac cgcgtgagaa
    12181 cacgacctgc cagagctgaa tatcacgggc gcggaaagca cctgcacagg cattcagata
    12241 ataggtaaac attcgtttaa agcgttcact atagttatcc gcaatttctg gccatgcggc
    12301 gaggaatcgt tcataccacg ccatcaacgt agtatcgtaa tcagcaccga agttatgcca
    12361 gtcttccatc acaaagtggg gttcgctgga ctgagcaatc tggcgtacag agggcaggca
    12421 accgttcgga aaaatatatt tattaatcca gggatcaaca ttcagatcgg tttttttcga
    12481 accgatagta tggagcagga atatgccttc cggtttcaaa ttacgatcca ccaccgcaaa
    12541 ataggtatcg taatttttcg gtccgacgtg ctcgaacatc cccacagaaa caatacgatc
    12601 aaactggtcg ttcaggtcac gataatcttg cagcaaaatg gtgacatcca ggccttcaca
    12661 gcgttcctga gccatttttt gctgttcggc agaaatggtg acgcccacca cgcttacgtc
    12721 ataattagat gccatgtagt gtgccagtcc gccccagccg cagccaatat ccagtacgcg
    12781 catccctggt tttaactgca atttttcaca aatcattttg agcttcgcct gctgggcaga
    12841 ttccagatta tcggcatctt tccagtaagc gcaggaatat tgcatgaagg gatcaagcat
    12901 gcggctgaac aagtcattac ccaaatcgta atgctctttg ccgactatcc aggcacgttt
    12961 tttactctgc agattgaaga gacgagcgcc ggcaatacgc agcgtgtctt tgaaatgatg
    13021 ggggagttgg ttctcgagac ctgcgcgtaa gactttgcta aaaaacatat ccagtcggtc
    13081 acattcccac cagccatcca tataactttc gcctaacccc aaagagcctt cttgcagaac
    13141 gcgtttaaaa aaatcggggt ttttcacacg aatatccgcc ggggcagaac cgttaatggc
    13201 tataccggca cggctaagta attcgttggc gatacggtac cagttgtcat ccggtacact
    13261 gacttcttct atacacgatg aactcatagt ttctccatca ctggtcggtg atcagaacct
    13321 taaaacagcg tagacgcttt ttgggctttg tgagaaatct cgcagaaaaa accgcgagtc
    13381 tataatccga cgtagaacag agggaaagga ggaacccttg ccgaaatgac tatccatagt
    13441 gagtcgggaa cgatcctgtc cccgtaaaaa cataatattt atcgtagctt taacaactcg
    13501 aaattagtat aggccgctaa tttcaatcat acaacaaaat tttgttagca gcctaagtat
    13561 aagtatatcg atatagatca gtgtgattcg ctatgagcga cttcggcatt gccatgattc
    13621 tggcagccaa cttcttcaca acgttgcatc atgtaaccca gcgcgaccag cactactgtt
    13681 gataacatca cgctggtggt ggtgagcaat ggcgtgctga tactgatcag ccaggaaact
    13741 accagacttg cgaggaagca cagacccagt tgaagagtgt tctgcaacgc tgcggcgcga
    13801 ccagttgcgt gtgggaaggg acgcagcgcc tgggcgacaa caatagggta gatcgcgcca
    13861 ttggcaatcg ccatcacaca gaatgggatc aggatttcga ccagcgacac atggctaatg
    13921 aagcccgcag cccaggtcgc aatgacgctg acagcaaaca gcaccagcaa ccacggtaat
    13981 aactgcttgc cttgccattt ctgcagcgcg gcgcgacagc cataaccacc aatcagaaac
    14041 gcgatagttt gcgggacata acttaaacca ataactgccg ggctgtagcc catttcacta
    14101 aggatgaacg gtgaaccggt cagccatgca aaaaaactgg ctgaacaggc tgcgtatatc
    14161 agcacgttgc cgcgataggt tttagaacgt agcaggtcgg taaaggtcag accatcctga
    14221 ctattgttac gggccttcgt cgtgggtttg agccagaaaa taggcagaat cagcaccacg
    14281 gtaatggcaa acagggtggc gaaaatcgcc tgccaggaaa aatggaccag cagccagctt
    14341 cctaacagag gagccagtgc cggagataga cccaccagcg gcatgatggt cgcaaaaata
    14401 cggttaactt tctgtgaagg ataataatct gtcactaatg cttgccagat aaccgccgcg
    14461 gcgcagacac ccacagcctg tacaaaacgc aataccagca gcgtagcggc gttttctacc
    14521 cacagcatcc ccagactacc taacgcaaaa attgtcaggc cgattaataa taccggttta
    14581 cgaccataac ggtcggagag cggcccccac agaagctggg ctgcggcaaa accggcaagg
    14641 aacagactaa ggctggcact gacagcagac gcaggcgttt gcaggtcggc ctgtatggcg
    14701 gcgaaagcag gcagatacat atcggttgcc agaaaaccga gtacgctcaa acccgccagc
    14761 cagactaaaa atcttttccc aggttgcatg tatattctct aaatctctca attaaacacc
    14821 gccgcaaagt gtagggagtg aaattagtgt tgtgaaacgg taatatttga tgactggttt
    14881 caaaaatttt gcaggcagac tatgtggtca gaatattcac tcgaagttgt tgatgcggta
    14941 gcgcgtaatg gtagttttag cgctgcggca caggagctgc atcgcgttcc ttctgcggtc
    15001 agctataccg tgcgtcagct ggaagagtgg ctggcggtgc cgctctttga acgacggcac
    15061 cgtgatgtgg agctgaccgc tgctggagcg tggtttctca aagaagggcg ctctgttgtc
    15121 aaaaaaatgc agatcacccg ccagcaatgt cagcagatag cgaacggctg gcgcggtcag
    15181 ttagctatcg cagtggataa tattgtcagg ccagaacgta cacggcagat gatcgttgat
    15241 ttttatcgcc attttgatga tgtcgaactt cttgtctttc aggaagtgtt caacggtgtc
    15301 tgggatgcgc tttccgacgg gcgcgtggaa ctggcgattg gcgcaacacg cgcgattccg
    15361 gtaggcggtc gttatgcctt ccgggatatg gggatgctaa gctggagttg cgttgttgcc
    15421 agtcaccacc cgctggcgtt gatggatggc ccgttcagcg atgatacgtt gcgcaactgg
    15481 ccgtcgttgg tgcgcgaaga cacctcgcga acgctaccta aacgtattac ctggttgctg
    15541 gataaccaaa aaagagttgt cgtgcctgac tgggaatcat cggcaacctg tatctcggca
    15601 ggattatgca tagggatggt gccaacacat ttcgccaaac cgtggcttaa tgaggggaag
    15661 tgggtagcat tagaactgga aaaccccttt ccggattcgg catgttgtct gacatggcag
    15721 caaaatgata tgtcgcctgc gttaacctgg ttgcttgagt atctcggcga tagtgagacg
    15781 ctgaataagg agtggctgcg ggagccggaa gagactcccg caacgggtga ttaacgacga
    15841 tagtcgcgga acgggccgtc agccacggag cggcgttcaa tcaagcgcgg atgcacttca
    15901 atagactgcg gttcttcacg tttgttgacg atacgatcca acagcatgtt gaacgctgtt
    15961 tcacccagcg aatcttttgg ctgatggatc gtggtcagcg ccggcgtaaa atagcgcgcg
    16021 ttgcgcacgt tatcataacc gatcagcgaa acatcctgcg ggacgcgcag gcccatttca
    16081 tcagcagcac aaagtgcgcc cattgccatg atatcgccac cacagaagac ggcagtaggg
    16141 cgatgcggct gcgacaggat ttgctgcatg gcgcgataac cggattcagg ttcaaagtca
    16201 ccctgcacaa tccagctttc cggcaccttg atcatcgctt cttccatcgc cttcataaaa
    16261 ccggcaaggc ggcctgcgcc ggtgttacgt tccagcgggc cggggatgac gccgatttcg
    16321 cggtgaccgc gttcaatcag ataacgcccg gccatgtagc cgccttcgaa cgcgttatca
    16381 atgaccgcat cggtgaagtc agcttttgct tcaccccagt ccatgaccac cattgggata
    16441 tggcgatact cttccagcat cgccagcaac ggctctgggt actcagaaca catcaccagc
    16501 agaccatcga cgcgtttttg cgccatcatc gacagataag cccgctgttt ctcaagattg
    16561 ttccacgcat tgcccagaat cagggtgtaa cctttctgga agcaattttt ttcaactgct
    16621 tcaatgatct cggcaaaata ggccgcttcg ctgctggtcg ccagcaaacc gatagacttg
    16681 gtgtggttaa ccttcaggct acgcgccacc gcgctagggg agtagtgtaa ttctttaatc
    16741 gctgcccaca cggcgttgcg cgtttcttca gcgacgaaac gtgttttgtt gatcacgtgt
    16801 gacacagttg tagtggaaac gtttgctcgt ttcgctacat cttttattgt tgccattcca
    16861 tttcactcca gaccctaact tcagctcctg caaaatcgca aggtaaacgt ttgcctttac
    16921 acaccttacg cacaacaaaa gtgttgcggt acgccggaaa aacgacacgg agcgtcagga
    16981 aggggtcaat ggccagtacg caaatagtgt aagcgtggaa ttttggccga tcttgacgaa
    17041 aaggggaagt ggtaaaaaca cttatcatta taaatgacgg gagatttttt catcacagtg
    17101 tgtaaaaatg cgtaatatca ctttttgtgt ggtaataaaa aaggagaaaa cttgatgagc
    17161 accgacctta aattttcact ggtaacaacg attatcgtcc tcggtttgat cgtagccgtg
    17221 ggtttgactg ccgcgctgca ctgatttcct ctcgagggag cgcgttctcc ctcgcatctc
    17281 cttcctgttt gttagcaaaa tggcaaaagt gttttctctt tttgttattt tcattttttt
    17341 gtgattgctg tcatgctttc gccactttaa tgaatatcgc gtaatgacgc gaagaggtgg
    17401 agataaggca tgaaaattaa ctatccgttg ctggcgctgg cgattggcgc gtttggtatc
    17461 gggacaacgg agttctcgcc aatgggcttg ttgcccgtca ttgcgcgcgg tgtggatgtc
    17521 tcgattcccg ctgccggaat gttaatcagt gcctatgcag ttggcgtaat ggttggcgcg
    17581 ccgctgatga cgcttctact ttctcatcgt gcccgccgca gtgcgttgat tttcctgatg
    17641 gcaattttca cgctcggcaa cgtactttcc gccatcgcgc cggattatat gaccctgatg
    17701 ctttcacgca ttttgaccag cctgaatcac ggagcatttt ttggtttggg ttcagtcgtg
    17761 gccgcaagcg tggtgccaaa acataaacag gccagcgcag ttgccactat gtttatgggg
    17821 ttaaccctgg caaatatcgg tggcgtgccg gcggcgacct ggttgggtga aaccatcggc
    17881 tggcggatgt catttctggc aacggcgggg ctgggagtga tttcaatggt aagtctgttc
    17941 ttctcattac ctaaaggtgg tgcaggggca cgacctgaag tgaaaaaaga gctggcggta
    18001 ttaatgcgtc cgcaggtgct gtctgcattg ctgacgacgg tactgggagc tggtgcaatg
    18061 tttactctct acacctatat ctctccggta ctgcaaagta ttacccacgc aacaccggtg
    18121 ttcgtcacgg caatgctggt gctgattggt gtcggattct ctatcggtaa ctatctcggc
    18181 ggcaaactgg cagatcgttc agttaacggc acgttgaaag gctttttgtt gttgttgatg
    18241 gtgattatgc tggcaatccc gttcctggcc cgcaatgagt tcggcgcagc tattagcatg
    18301 gtggtgtggg gcgcagcaac ctttgcggtc gtaccgccgt tacagatgcg cgtgatgcgt
    18361 gtcgccagtg aagcgccagg tctgtcttca tcagtcaata ttggtgcctt taatcttgga
    18421 aatgcgctgg gagcagctgc tggtggtgcg gtaatttccg ctgggctggg atacagcttt
    18481 gtgccggtga tgggagcgat tgtcgcggga ctggcattat tgctggtgtt tatgtcagcc
    18541 agaaaacaac ctgaaacagt ttgcgttgct aacagctaat taaacaaaaa cgcagaagga
    18601 taaccttctg cgttacagtt gctgacaacg tgcgatttat cttgccggat gcggtgttaa
    18661 cgactcatcc gcgttgacat gaaaagacgc gttaggcgtc gtatcatgca gcgcttgtcg
    18721 gatgcggcga gtgccttatc cgacctacat cgatgatccg cgtaggcctg ataagcgtag
    18781 cgcttcaggc aatgctgcat ttgccatcag ttattatgca gcgagatttt tcgctacgaa
    18841 ttcccagttc accagcgccc agaagtgctc cagatagcca ggacgtgcat tgcgatagtc
    18901 gatgtaataa gcgtgttccc agacatcaac ggtcagcagc ggagtcgcat cggtggtcag
    18961 cggagtaccc gcgttagagg ttgaaacgat agccagtttg ccatcgctgt ttttcaccag
    19021 ccaggtccag ccagaaccaa agtttttgat cgctgcatca gtaaactgcg ctttgaaatc
    19081 ggcaaagctg ccaaaagatg cggcgatagc ttcagcgact tttccagtcg gttcgccacc
    19141 ggcgttcggt gccaggcagt tccagtagaa agtatggttc cagacctgag ctgcgttgtt
    19201 gaatacgcca ccttcagagc tgcgaataat ctcttccagt gatttacctt caaacgcggt
    19261 acctttaatc aggttgttca ggttagtgac ataagtctga tggtgcttgc cgtagtgata
    19321 ctcgatggtt tccgcagaaa tgtgcggtgc cagagcatct ttagcatatg gtagtgcagg
    19381 taattcgaat gacattgcta ctctccttta ttattaattt gcatacgtac aatagcctta
    19441 ttgtgcgtat ggatagggta gcaaattaaa aggtgagaca aaagatgaac ttaccctgtt
    19501 gccgtaacaa cagggtaaag ttttagcgaa gtgtttttgg ggtcattacc cgacgagcgc
    19561 caacatagtg gcgctgccag tagtcttcac tgagagaagt gatttgaatt tcctgacctg
    19621 tgcgcggtga ctgaataaat ttgccgttgc cgacatacac gccgacatga tcggctgtgc
    19681 cgcgtccctg agtacggaaa aagaccaggt cgccgttttt cagttcacta cgttcgattg
    19741 gcgctgcatc acgcaggtga tacatttcat tcgccgtacg cggaatacga attttcacca
    19801 aatctttata agcgtaataa accaggccgc tgcaatcaaa accggtacgc ggtgagctgc
    19861 caccccaacg atatggctta ccaatttgct gcatcagttt attcattgcc actttcgtag
    19921 ctttttgcac cttcgctttg tgcgcgtcgg caagagttcc tgacgcggca tttttgactt
    19981 tcacacaatg cgatttatag cccttacgtt tggtacactt ttcggtaaag ctggctgagg
    20041 ccgttttatt gacggcgtgt ttgctgcgag ttttggcgtt tttagaagcg gtaatggaag
    20101 attttttaac ggaagaagcg gttttgcttt tggtcgtact tttactggcg gcttttttcg
    20161 ctgttttctg ggtctttttg gtggttgccg tgcttttctt tttatctgct tttttggtga
    20221 tatgagtggt agcagaactc tccctggctt gctttgaagc atgggccata ggcgttaaag
    20281 gcagggtggt aaaaagtaaa gcacagagcg tgatcgaaat acggtttatc cgcgccactg
    20341 agcgtccctc tgttaaggca ggtctatcat gcctgcgtct gatttacaaa acttgatgat
    20401 tctaatgcat agaagaagag aaagttaata gcttttattg ttaaaaaata cgtttcgttg
    20461 gcgagcgcaa aaaaatgcag cagtgaaagc taaaaatggt tataaatacg ccagtttgcc
    20521 ggcatcagag aagaaacgaa tatttgaaat gcaacaaata gagcaaaggg ataaaatagc
    20581 aaaagcgtga tgaaaatgtt attttccgat gccgcactga cccgctacaa tgtcagatta
    20641 ctgccgtaaa gaagtttgag gaagcaagac aatgagcacc actatcgaaa aaatccaacg
    20701 ccagattgct gaaaacccga tcctgctgta catgaaaggt tcaccgaaac tgccgagctg
    20761 cggtttctct gcccaggcag tccaggcgct tgccgcatgt ggcgaacgtt ttgcctatgt
    20821 tgatattctg cagaatccgg acattcgtgc ggaactgccg aaatatgcta actggccgac
    20881 cttcccgcaa ctgtgggttg acggcgagct ggtcggcggt tgtgatatcg tgatcgaaat
    20941 gtatcagcgt ggcgaactgc agcagctgat caaagaaact gccgctaaat acaagtctga
    21001 agagccggac gcggaataat ccgtcctgca atactgacgg atgcgaacgg gcatccgtca
    21061 gtcctttctc tatccccaat ccagcccttg tggcgaactt gaaaatcccg cctcacgcag
    21121 cagcgtaaac atcggcgttt gccggaccgg tagatcattc acttctgtta gcgtaaagcg
    21181 cagccgtggt tcgcgacgca gtgcggtagt cagcgcgtgg aaaacctctg gggcgagtaa
    21241 ttcctctttt tcctgccaga ccagcatttt tttgccacct tgcgccagat agagtaacaa
    21301 tttgccgcca gaaacgacca ccagcgcacc cgcccgacgc gttggaacca gcgttgccgg
    21361 atgtgcaggc cagggcagaa gatttcccca cacattagcc ggatcgttgg cagagagcgc
    21421 cactggtgta tagtggcgcg tttgcgtggc ttgtgtcgcc agatcgcgca atcggtcaat
    21481 agtcagacgt tcagcgaatt gcgcgccacc cagaccttct acaaaacgac ctcgcataat
    21541 tcgcccggag tcttccatac ttcgacaaag cgtttgcatc gatggaaacc cgccagggat
    21601 attttcggct atcaccgcct gacgactgat gatgccgtag cggtcgagca tattttccgc
    21661 cagcgccagc atcctttcgg tatcgtttag tggctccacc tgcaataacg accagcgtcc
    21721 agccagattt ggtgtgttgt aagatacccg cggcgagacg ggttgcgcat agacaggacg
    21781 tccacgacga gcccggtgac tgcggcgagt tgaggtgcgt gcgttggaac tgctgcgggt
    21841 gagggcgcgt aacggtgccc aaatgtcgct ggtgatgacg ccttgccaga ccagcgccca
    21901 taacgcctct tgcagggcag agagatcaac cgattcgccg attttgtcgc gtatccgttg
    21961 gctgatttgt tgtgcaaacc aggctcctcc gtcagccaga acagcgacta tcgcttgttg
    22021 cagcgccgaa cgattcgcct gatccgcttc ggcgggagtg aacgattctg cagcatattc
    22081 ctgtagatgc agtgccacca ggccgtcatc ttcacccagt tttttttgcc ccgaccagat
    22141 aaccgcaccg gttgccagta attcatcgag catttctgac gaatagtcgc gtacgcgggc
    22201 aggcaggatc tggctttccc agagtgaggc gggtaaaccg actccggcaa gctgttcgat
    22261 cacccgcatc acgccatcta cgccctcata aacgcctggc gatgttgagg caaagagcgc
    22321 cgggctacca tcggtggcgg gtaatacgcc ctgacgttcc agcagcaatc gcgcataggt
    22381 cgtggctgca acgggacgcg tcgcttctct ggcggcttgc agcgagcgca aacgcagacg
    22441 acgaaatact tcatcgctca cccagatgtc ttgttgcaga ttcatcacca gaccctgttc
    22501 acgcagttgc tgaagctgct cttcgacaat ggcaataccg agactaaact catgagccag
    22561 ttgttcagcc gtgaccagag catgagcccg gagatagcgc agaaagaggt cgcgaagcgg
    22621 gtaactgact ctatgtaaat aaatctctgg caatgactct ggtagtcgta cgccgtgggc
    22681 atcacgcagc ctggcggcat catccataca ggcaagacgc tcctgtccgc taatcatcgc
    22741 tgggaagatt cgttttactg caagaagatt ttccagatac gacgcaacct cttcactgct
    22801 gcctgtatgc cgttgcgcca ggtcttcaac ggtcattggc cccagttcgc gcagcaggtc
    22861 gaacaatcct tcttcacctt tcgctcttct gccaggagcc agtcgttgca actcttcttc
    22921 cacctggcga atgacctgcg ggtcgagtaa ttcccccgga tcgacctgtc cgagtagatt
    22981 gcgcagtaac tcgctgtcca gcgacagtac ggatgcccgg cgctctgcca gcggggcgtc
    23041 gctctggtac ataaattccg cgacatagcc gaacaataaa cttgtggcga aaggcgaggg
    23101 cgtagtggtc gttacatcgg atatttgaat ttcgccaccg ttcaggcgac gcatcaaacg
    23161 ttccagtgcg ggaagatcat aaacatcttg cagacattcg cgtagggttt cgagaatgac
    23221 cggaaaatcc ggatatccct gagcgatttc cagcaactga ctggcgcgca gccgttgttg
    23281 ccaaagcggg gtgcgatggc ccggagtgcg ccccggcatt aataatgccc gcgcggcgca
    23341 ttcgcgaaaa cgggcggcga aaagtgccga gctgcctacc gcctcgcgga caatttgcag
    23401 caacttttct ggttcaaaca aaaaaatcgc ggcatcgggc aatttaccat cggtgtcagg
    23461 aatacgtgca acaatgccgt catcactggc gaccaccgac gcgtcagcgc cccatagcgc
    23521 atgtattcgc ccggcaatcg ccaccgccca gggttcatgc acccgtcttc catagggaga
    23581 gtgcaaaata atacgccagt caccaatttc atcacggcac cgttcgagga ccagatggcg
    23641 actccccgga acgatgcccg tcgcgttgcg ctgctcctca atcaacccct gaatattggc
    23701 gatcgtattt tcttctgcca gccacggggg aatagtcccg gaaaagaacg cgccatccgc
    23761 cagcaaatga agaaaatcgc cgatcatctc gcctaattca gccggacgtc cgttaccttc
    23821 accacgccag aaggggagcc gggcagaacg acccggagca ggagtcacaa tcacctgatc
    23881 gcgggtgatt tgctggatcc gctatgaggt agcgccgaga gtgataatgt cgttcacccg
    23941 cgactcatat accatctcct catccagttc acccacccgc cgcgaaccgg ccttttcttc
    24001 accttcgggt aataacacgc tatacatgcc acgatccgga atggtgccgc cgctggtaac
    24061 cgccaacaat tgagcgccag gtcgggcggt caatatcccg gtctccctgt tccagaccag
    24121 tttggggcga aaagcagaaa aatcgccaga gggatagcgc ccggaaagca tatccagcgt
    24181 ggcgtcaaaa acacgtcttg gcagatcttt ccacggtgcg gcacggcgta cgcgggagta
    24241 ccattcgtct acctgtaatg catccatcgc cgcggcggca acggtttgct gcgcaaggac
    24301 gtcgagagga ttatgcggtg gtgtcaggtt ttccagcctg cctgcgaaca tacactctac
    24361 aatgactgcg gaatcgacta aatcacgccg ggtacggggg aaaaacagcc ctttagatac
    24421 accgccaacc tgatgtccgg cgcgaccaat gcgttgtaac ccactggcaa cagaaagcgg
    24481 cgttgccacc tgaatcacca gatccaccgc gcccatatca atccccagtt caagactgga
    24541 ggttgcgacc acgcagcgta attcacccga tttcagcgcc tgttcggtga ttgctcgttg
    24601 ttctttagag acggagccgt ggtgtgagcg ggcaataaaa acgtcgctac tttgtacacg
    24661 gttagaggtt gcgccggagg tcgactcgaa atgggccgca tcaacggcga tagacgggga
    24721 acgctgtaag cgtgcggcgt aaagctcatt taatcgtgcc gtcagttttt ccgccagccc
    24781 ccgcgaatta gtaaagacaa tggtcgagcg atggcgcaac acttcatcaa ggatacccgt
    24841 ttcaatatat ggccagatgg agccttcccg gccggcatgg ctgtcttcgc cggtgccgct
    24901 ggcgaccgat gagacatcat ccatattggc gaccggtacg acaattcgta tctgcggatg
    24961 gcgcattgcg ggcgggttga ctaccgtaac cgggcgatcg ccaccaagaa atgctgccac
    25021 atcgctggct gagcgcacag tggcagaaag gccaattcgc tgtgctgagg tgtggagcag
    25081 cgcatcgagc cgctccagac ttaacgccag atgcgcacca cgtttactgc ccgctaccgc
    25141 gtggacttca tcaataatta ccgtttcgac gccgcgtagc gtttcgcgcg cgcgggaggt
    25201 cagcatcaga tagagtgatt cgggtgtggt gatcagaata tccggcggat tacgggtgag
    25261 tttgctgcgt tcctgtgcag gcgtatcgcc agtacggatc cctacgcgaa gattgacttc
    25321 cgtttcgccg cgccgccgcc gttcatcggc aatacccttc aacgggatct gcaagttgcg
    25381 ctgaacgtcg gtgcccaggg cttttatcgg tgaaatatag aggatgcgtg aggttttacg
    25441 cttatgcgcc tcgcgggtat cttcgccgcc ttcgcggaag agccgatcga gggcgtagag
    25501 aaatgctgcc agcgttttcc cggagccggt cggtgcaatc accagcgcat gttcgcttcg
    25561 cgccgccaca tgccaggttt gcggctggac agcggtcggc tgtttaaagg cgcgaagaaa
    25621 ccagtcgcgg gtcgccggtg aaaacacgtc cggcaggagc gatgaagggt ctggattatc
    25681 tgccattaac gctattcccc gtgaatatca tgcaggatgc gtggaatagc ctcactatag
    25741 atgcctgaat cattgcatgt catcaggcat cgactcgatt acacctcttc ggcggcagat
    25801 agcggccagc ctcccagacg tttccagcgg ttgacgattt cacaaaacag cacagcagtg
    25861 cgttcggtgt cgtacagcgc ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc
    25921 tggcaagcct ttgacaatac ggtttgtccg agtgccagcc cggccagtgc agcagtgtca
    25981 aaagtggcga aagggtggaa cgggttacgt ttcagtgagg cgcgttctgc ggcggccatc
    26041 ataaagctgt gatcaaaatt ggcattgtgc gccaccataa tggcgcggtt acagccgctc
    26101 gctttaatac ctttacgtac aactttaaaa atttcgtgca gcgcctcgta ttcgctgacc
    26161 gcgccgcgat cgggatcgtt cgggtcaatg ccgttgaagg cgagggcttc tggttgcaaa
    26221 tttgcgccga caaatggttc gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct
    26281 tgttcatcca ttttcagggt gatggcggca atctcaagca gcgcatcggt tttggcgtta
    26341 aatccggctg tttcaacatc gatcacaaca ggataaaaac cacgaaaacg gtcgcacaga
    26401 ccggtaagtt gagcgttatc ggacatctga ttctcttaat acggataaat tgcagcgcgc
    26461 attatgacaa atattacttg tcgatgcagt aaagatgcgg gcgcgatgag ttcacgcccg
    26521 gcaggagatt agttgcccag accgcgaccg gcgtctttct cttcgattaa ctcaattttg
    26581 taaccgtccg gatcttccac aaacgcgata accgtagtac cgccttttac cggacccgct
    26641 tcacgggtca cgttaccccc gttttgacgg attttttcgc acgcttcagc ggcgttatct
    26701 acgctaagcg cgatgtgacc ataagcagtg ccgagttcgt atttatccac gccccagttg
    26761 taggtcagtt caatcaccgc ttcttcggtt tccgggccgt agccaacaaa cgccagtgag
    26821 tatttgtatt ccgggttttc gctggtacgc agcagtttca tgcccagcac tttggtataa
    26881 aaatcgatgg agcgttgcaa atcgccaacg cgcagcatgg tatgaagaag acgcatcttt
    26941 ttatcctcaa aatggctaac agattcaaaa tgttgtttta gtatagcggc ggctttacgc
    27001 cgctcgcaat gttggattac aacgtcgggt aatcggtata gccttccgcg ccgccaccgt
    27061 agaaactttc ggcacgctgt gggttaagct cagctttgcg ctgcaagcgg gcgaccagat
    27121 ccgggttcgc aatccagtca cgaccaaatg ccaccgcatc aattaaccct ttgccgatca
    27181 gcgtttcagc tttttctact gtgtatgcac ctgcgccgat aatcggaccg tggaaacggg
    27241 cgcgtacttt ttcgcggaac gcatcagtat acggttcacc ccccgcccaa tctggttctg
    27301 acatatgcag ataagcaatg ccgcgtttac ccagttgttc aatcagatac agtgcatcgg
    27361 cttcttcatt cgggccgtta tctgtgttct ggaaagtacc gattggtgaa acgcgaatgc
    27421 caatgcgatc ggcaccccat tcttcaatcc cggcatcgac cacttccagt accaaacgtg
    27481 cgcgattttc cacgctgccg ccgtactgat cggtacgatg gtttgaagaa ggagaaagga
    27541 actgatgcag caaataaccg tgagcagagt ggagctctac cagatcaaaa ccggcttcac
    27601 gcgcgttagc aatggcctga cggaaatcat tgacgatacc tggaatctct tccagttcaa
    27661 gcgcacgcgg catggatgtt tcaacacgga tcgcctgacc attttcatcg cgcagagaag
    27721 tacgtgttcc cgcgctaagt gctgaaggcg ctaccggtgc ctgaccgcca ggttgcaggc
    27781 tggcgtgaga aatgcgtccg gtgtgccaca gctgcacggc catatgacca ttttcagcat
    27841 gaacgccagc ggtgattttt ttccatgcgg caatttgctc cggactatgg atgccaggcg
    27901 cacctgcata tccttttgcc tgggcagaaa tttgcgtggc ttcactaata atcaaaccgg
    27961 cactggcacg ttggcgatag tattccgcca tcaacggggt aggaatgtca cccggttcaa
    28021 tactgcgcag acgcgtcagc ggtgccataa aaatacggtt tgccgccgtg atcgcgccca
    28081 ctttcagtgg ggaatacagt ttttcagatg acataaaggc tcccggtcgt ctggtgataa
    28141 aaaatgcctg ctaaacggca ggcgtcgcaa taatgttttt tacatgggcc agcgcgtttt
    28201 ccagtggctc gaaactgcgc gaaattttgg cctgcagatt cgcgccaagc cacagtgcgt
    28261 aaagcacttg tgcctgttgc agcggttcgc cacaaaaggt taaacaatgg ttctcacggc
    28321 cattttccag cgcctgagag agcagggcga tcacgccgcg agcaccttta tccatcgcgc
    28381 tgcgcatatc ttctgacaga tcgcacactt cggcagagag ttttactgtc aggcaaccac
    28441 tgatggttcc atgttggcaa aactggttca gtgtttgctg gtaataagcc agtatgcggt
    28501 cgcggtagtt accttcgccg gattgcagca actcagtcag tcgctggtga tatgcggcgt
    28561 aatgacgctc aagcatggca acgccaaacg cttctttaga gcgaaagtag tgatagaagg
    28621 accctttcgg cacttcagcg gtttttagta attcgcttag ccccatcccg gtgaatccac
    28681 gttgcaggca aagttgctcg cccgtcgcca ggagatgttc gcgagtatca tgttcggtgt
    28741 gtttgttcat gcgttggagt gtagtagacc agtcggtcta gtgcaagcaa agttgctact
    28801 ttggacatta acagcttcaa taatggtaat aatcttatct gaggagggcg aagtggcgga
    28861 gcaattagag ttctttcccg tccagagccc gtgccgggga atttgccagt ctgacgaacg
    28921 cgggttttgt cgcggctgtt ttcgtagtcg tgatgagcgt tttaactgga ataaaatgag
    28981 cgatggcgaa aaacaagagg tgctgcgcct ttgtcggcag cgattaatgc gcaaattacg
    29041 cgcaaataag ccagcgtcat cagacgaacc ggagcaacca tcactctttt gaacctaatc
    29101 ttgcgtatac tcctctcata tctgataaga ggaagcgatt atggttcagc gtattactat
    29161 tgcgccgcaa ggcccggagt tttcccgttt tgtgatgggc tactggcgat tgatggactg
    29221 gaatatgtcc gcccgccagc tggtcagttt tattgaagag catctggatc tcggcgtgac
    29281 caccgtggac catgctgata tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc
    29341 actgaaactg gcacctcacc tgcgtgaacg gatggaaatc gtcagtaaat gcggtatcgc
    29401 gacgaccgcg cgtgaagaaa acgtcattgg tcattacatc actgaccgcg atcacatcat
    29461 taagagcgcc gaacagtcgc taattaatct cgcgaccgat catctggatt tgctgttaat
    29521 ccaccgacca gacccgttaa tggatgccga tgaagtggcg gacgcgttca aacatctgca
    29581 tcagagcggc aaagtgcgtc attttggcgt atcgaacttt acgcctgcgc aatttgccct
    29641 gttgcaatca cgtctgccgt ttacccttgc cactaatcag gtggaaatat ccccggtgca
    29701 tcagccgtta ctgctggatg gcacgctcga ccaactacaa caactgcgtg ttcgtccgat
    29761 ggcgtggtcc tgccttggtg gtggtcgtct gtttaatgat gattatttcc agccgctgcg
    29821 tgatgaactg gctgtggtgg cagaggagtt aaacgcgggc tcgattgaac aggtggttta
    29881 cgcctgggta ttacgtttac catcgcagcc gctgccaatt atcggttcag gtaaaattga
    29941 gcgcgtacgg gcagctgtcg aagcagaaac actgaaaatg acccgtcaac aatggtttcg
    30001 tatccgtaaa gcggcactgg ggtacgacgt accgtaagca cttttaggaa tagccgccgt
    30061 tcaaaaatgt gtcactggtt tacacttatt caggaatgca caatgaacgg aggtcctatg
    30121 aaacgtttta gtctggctat tctggcgctg gttgttgcaa ccggcgcaca agctgccagt
    30181 gaaaaagtcg agatgaacct cgtcacgtcg caaggggtag ggcagtcaat tggtagcgtc
    30241 accattactg aaaccgataa aggtctggag ttttcgcccg atctgaaagc attacccccc
    30301 ggtgaacatg gcttccatat tcatgccaaa ggaagctgcc agccagccac caaagatggc
    30361 aaagccagcg ccgcggaatc cgcaggcggg catcttgatc cacaaaatac cggtaaacat
    30421 gaagggccag aaggtgccgg gcatttaggc gatctgcctg cactggtcgt caataatgac
    30481 ggcaaagcta ccgatgccgt catcgcgcct cgtctgaaat cactggatga aatcaaagac
    30541 aaagcgctga tggtccacgt tggcggcgat aatatgtccg atcaacctaa accgctgggc
    30601 ggtggcggtg aacgctatgc ctgtggtgta attaagtaat taagaggcca gcgtaccttg
    30661 cggtggtgct tgctcaagtt gcgaaagcga gcagtacagc cgccagacaa ttgccgccag
    30721 ctcacgggcg gcaggttgat gatgacgggc aaggctgtcg caaatccgct gtaattcttc
    30781 aagtgtggcc gccagtgatt tttgctgaac gccacgctca ctcatcactc cccgcaacag
    30841 tgaaatacag ttatcccgta ctcgcgataa cggatcggaa cgcgattccc agtcgcgcaa
    30901 ttgccagaca acatgagaac agttcagcag cactacaccc cagcgtaata accaacggcg
    30961 agccagcgca tcctggctgt tactcaactg actgacgtga tgataagtga gcgattcaaa
    31021 ttcgctttca ctcagtgttg gatggcggct tagctgatcg acaaaatccc ggcgcagcgc
    31081 gcgaatatgg cggcggcttt tacgagcatc cgatcctgga cgcagaatgg cgaacgctaa
    31141 ccacgccaac gcgacgccaa cgatttttgc cagattatcg ttaagaaaat cagcaaaatc
    31201 atacaccggg ggattagtga cagcgataaa agaacccata aaaacaatca gttgccccca
    31261 caatgcggca aattttggca tctgcaattt aagaagctgc attgtcgcca gcagtggaaa
    31321 gagaaacagt aaaaattgcc acagatcgct aatctggacc atcagaccaa atttgaccac
    31381 aaagctgaat agcgaaagta acaccagcgt gcgcatcaga agtgacaacg acttaaacgg
    31441 tgctgcgacg gcggagtaga gtacgcagct aattgctgcc agcgttaatg cattggcacc
    31501 ggcatcccat tgcgaagcaa tactccatgc gccaatcatc atcaacgtac aaaatgtacg
    31561 cagcccgctc cacatagctt cggcgttatc ggtatgacga gccaggccgg atgtccgtgg
    31621 gagtctggta tgatcatcta caccgctttg cagacgatgt aattcctgac tactttgcag
    31681 ataaaggcgg caaaaataac gtagtcgctg ccagaaggcg acgtgccgat agtcggcgac
    31741 gttggtcggg cgtagcgggg cgataatacg tgcgacggtg taaacatctg tttgcgaact
    31801 ggcgagcgcc gtcagcaact gttcgagaat ttctcgtgtg gcacctggcg gtgagggcca
    31861 gttgagcaac atacgtcgca ggctggagat gacactggtc atacgtaatt gctggtggag
    31921 cagcgcatta aggcgcgcgt tttgctggcg aaaacgatag tggctccaga aagcctggat
    31981 acgcagcaaa ttcatggtca gtatctgccc aatcacccct tcatgtgctg cacgaatggc
    32041 atcggttgtt tcaggctgcc agagtaaact ggcatgttcc agtaatcggg cgtgcatgtt
    32101 tttcaatgcg gttaaaaggg cagtagcatc ggaactgctc ggcaggatca tcatcatcat
    32161 gccgccgcac aaaataccga ctattacctc gcaaacgcgc gcctgagcga tatcccacag
    32221 ctggctggcc tcagtaatat taaccatcgg aaaggcgatg atggcagccg tgtagcccgc
    32281 cagttgaaat gcatacgcga cgttattcgt gaagtgcgca caggcccagg tacaaaagcc
    32341 aagccacgcc gacatgctca atagaaaaaa ccacggctca ttgagcgtat gcccggcaag
    32401 aagcagtgcc gcaatggctc cgagcaaact gccagcgatg cgtccgaggc ttttgctgat
    32461 aacaccgcca acggtgggaa agctaaccac tgcagccgag gtcatcgccc aatagggttc
    32521 atccagattt aaataatagg caaccgtcag cgccagacac atggcaatgg tattgcgtaa
    32581 cgcataacgc cattgcgcca gcgtggccct gaaccagggc aaattgcgca aggaccatga
    32641 cgatgcgttc attattgacc tacggcaatg ctacaggtgg tgcccgacac caacgtgaca
    32701 tcgcctggga ctttatccag cgcaaagcgg acgggaacgc gttgggcgag tcgaacccag
    32761 gggacgttag gtttaacatc cggaataagg cttgagtcac tttcaacgct ctgatcataa
    32821 atcgcccgac cgatactgga aacgtgaccc tgtaacgttt tgttatcact atagagggta
    32881 atttgtgctg gtgcaccttc gcggatatgg cgcaacttgg tttcttcaaa ataaccaatg
    32941 acgtaaaacg agtggctgtc gacaagggca aacagcggtt tcccggtatc agcgtaatcg
    33001 ccgatgcggg tagtaaggtt agtcacccat ccactcaccg gggcgcgaat ttccgtttgc
    33061 gccagttgcc attgtgcctg tttcagcgtc gcctgtgcgg catcaacact ggcctgcatc
    33121 gctttaacat tgaggttagc ggtatcgagt tcttcggcag aaataaaatt ttgtgagaga
    33181 tgacgacgac gattcgcttc gttattggct tttgcgaggt cagattgcgc tttcgccagc
    33241 tgagcctgtg cgttcagctc ggcgatctga aagggcgttt tatcgatcgt gagcagaaga
    33301 tcccccgcgt taaccagctg gttgtctttt atattcagct caacaatacg tcccgacacc
    33361 tgtggggtga tagaaacctg ttctgcgcgt attttcccgt cgcgtgtcca cggagattgc
    33421 atgtaataat tccacagcca ccatccggca agaaccgcaa ctaccgctac aatgattgtt
    33481 gagaaatact taattgtttt aattgacata gttaccacgc aatcagtatt gccagagcaa
    33541 ggcaaacgca aatcgcaaac agcgataaat ccattaacaa gggatgccag atgtcaccgg
    33601 cgtagatcca gccacgaagc aggcggtgta cgacaagcca gatgacgaat ccaaacgcga
    33661 atgctttgaa aaacggagga aagtagacgg acgcaccgaa caccagatct tgtaagggca
    33721 atcctgttgc attgagcata aacttcacaa agtagattcc tttacgaccg aatatattgc
    33781 gttagattaa taaatattct ttaagtgcga aaaatttacg cgcaatttct gaaaaatatg
    33841 tctgacggta accaaatgca gcaatacatt tgttttagca atacaattgc tgcacactat
    33901 tctaaaagcc gcataatatc ttagcaagct aattataagg agatgaaatt ggaatcgcca
    33961 ctaggttctg atctggcacg gttggtgcgc atatggcgtg ctctgataga ccatcgcctg
    34021 aaaccgctgg agttaacaca aacccattgg gttacgttac acaatatcca tcagttacct
    34081 ccagaccagt cgcaaattca actggcaaaa gcgattggca tcgagcagcc atcactggtc
    34141 cgtactctgg accaactgga agaaaaaggg ttaatttcgc gtcaaacttg tgccagcgat
    34201 cgtcgggcta aacgtattaa actgacggaa aaggcagagc cgctgatcag cgaaatggaa
    34261 gctgttatta acaaaacccg cgcggaaata ttacatggca tctccgcaga ggaactggag
    34321 caactgatta cgctcatcgc aaaacttgag cataatatca ttgagttaca ggccaaaggg
    34381 tgaaatgaag ggggcgtgtg gtcagtaacc tgaccacacg caaacttatt tagcgcggag
    34441 aaacggtcac ctgactgcca ttgctggcca gtacgacacg ttggcccgga gagaaacgag
    34501 tgttgccttg tttctgtacc accatgatgg tattaccatc gtctttacga atttccagct
    34561 cgacaccctg cgttttgttc attgcactct gtacgccctg accagctacg ccacctgcaa
    34621 cagcgcctgc tgcagtagcc agagaacgcc cggttccgcc accaacagta ttccccagga
    34681 aaccaccaag aacagcaccg ccaattgcac cgataacgtt ggaatcatca ccgccctgaa
    34741 tctgtaccgg acgtacgtta acgatggtgc catagctgac attctgtact tgtttcgctt
    34801 cagaagcggt ataaacatcc cctgacaggg tgtcgttatt aacacaaccg acaagagaca
    34861 gacctaccat tgaaacaacc aatacgcgtt taatcattga aaaatctcct gttcaccatg
    34921 aaacgccacg caagcatccc tcatggtcaa agtatatggc atatgtgaat ccatggttac
    34981 atgttctacc aaatatgagc aaatcataat gaaagtagga tcaaccaatt ataaacaaaa
    35041 cattcatgat gattattgat ttgaagataa tgttttacta ccaacggtaa aaatcggtca
    35101 ttccgcatgg tattcacgaa aagtttatgt ttgagtggtg cgcaaacgtc agattgaaaa
    35161 aggatgaact atgaaatcgg gccgctttat tggcgttatg tcaggcacca gccttgatgg
    35221 tgttgatgtt gtgttggcga caattgatga acaccgggtc gcacagctgg caagtttgag
    35281 ttggccgatc ccggtatctc tgaaacaggc tgtactggat atttgccagg gccagcagct
    35341 tacactttcg cagtttggac agcttgatac tcaactcggg cgactttttg ctgatgcggt
    35401 caatgccttg cttaaggaac aaaatctgca ggcaagagat atagttgcga tcggttgtca
    35461 cggtcaaacc gtctggcatg aaccgacggg cgtggcacca cacactttac agattggcga
    35521 taacaatcaa attgtggcac gcaccggaat tacggttgtc ggtgattttc gccgtcgcga
    35581 tattgccttg ggaggacaag gcgcaccgct ggtacctgcg ttccatcatg cgctgctggc
    35641 tcacccaacc gagcgacgaa tggtgctcaa tattggcggc atcgccaatc tgtcactgct
    35701 cattcctggg cagccggttg ggggctacga taccggtcct ggtaacatgc tgatggatgc
    35761 ctggatctgg cgtcaggccg gtaaacctta cgataaagat gccgagtggg cacgggcggg
    35821 taaagttatt ctcccactgc tgcaaaatat gctcagcgac ccgtatttct cgcaacctgc
    35881 accgaaaagc accggacgcg aatactttaa ctatggttgg ctggagcgcc atttgcgcca
    35941 ttttccgggt gttgatcccc gagatgtgca ggcgacactg gcagaactca ccgccgtgac
    36001 catttctgaa caagttttgt tgagcggtgg ctgcgaacga ttgatggtat gtggtggagg
    36061 tagtcgtaat ccgctactca tggcgcgtct ggcggcatta ctgccaggca cagaagtcac
    36121 caccaccgat gccgttggca ttagtggcga tgacatggaa gcattggctt tcgcctggct
    36181 tgcctggcgg acgctggcgg gattaccagg aaatctgcct tccgtcactg gcgcaagcca
    36241 ggagacggta ctgggggcta ttttccccgc taacccgtga tatcagagtt aactgaattt
    36301 ttcttaccgc gtctgccgtt acacttggaa cgttagggag ggcgtattgc cctccagacc
    36361 aggaaagtct tcgggatatg actatgaaaa aactgttaat tatcattttg ccggtccttc
    36421 tctccggctg tagcgccttt aatcagctgg ttgagcgcat gcaaaccgat acgctggaat
    36481 accagtgtga tgaaaaaccg ttgacggtca aactgaataa tccgcgccag gaggtcagtt
    36541 ttgtttacga taatcaacta ctgcatctca aacagggcat ttcagcctct ggcgcgcgtt
    36601 acactgacgg aatctatgtt ttctggtcga aaggcgatga agcgactgtc tataaacgcg
    36661 accgcatcgt cttgaataac tgtcagttac aaaatccaca gcgttgagat ttttccaggg
    36721 gcggcgcaca atagcgtcac ccactgacaa tccgtaaaga aaaccatgtc tgataacgac
    36781 gaattgcagc aaatcgcgca tctgcgccgt gaatacacca aaggcgggtt acgccgccgc
    36841 gatcttcccg ccgatccatt aacccttttt gaacgttggc tctctcaggc ttgtgaagcc
    36901 aaactggcgg accctaccgc gatggtggtc gctaccgtgg atgaacatgg tcagccttat
    36961 cagcgcatcg ttttactcaa acattacgac gaaaaaggca tggtgtttta caccaacctc
    37021 ggcagccgta aagcacatca aatcgaaaat aatccgcgcg ttagcctgct gttcccgtgg
    37081 catacccttg agcgccaggt gatggtgatc ggtaaagcag aacgactttc gactctcgaa
    37141 gtgatgaaat attttcatag ccgcccgcgt gatagccaga ttggtgcatg ggtttcgaag
    37201 cagtccagtc gcatttctgc ccgcggtatc cttgaaagta aattcctgga gctgaagcag
    37261 aagtttcaac agggcgaagt gccattgccg agcttttggg gcggttttcg cgtcagcctt
    37321 gaacagattg agttctggca gggtggtgag catcgcctgc atgaccgctt tttgtaccag
    37381 cgtgaaaatg atgcgtggaa gattgatcgt cttgcaccct gaaaagatgc aaaaatcttg
    37441 ctttaatcgc tggtactcct gattctggca ctttattcta tgtctctttc gcatctggcg
    37501 aaaagtcgtg taccggcaaa ggtgcagtcg ttatatacat ggagattttg atggcaagca
    37561 gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg gacgaggaag
    37621 cgttagcaga gcgactggcg caaggcccga tcgcgctcta ttgcggcttc gatcctaccg
    37681 ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc ttccagcagg
    37741 cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc gacccgagct
    37801 tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg gtggacaaaa
    37861 tccgtaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct gctatcgcgg
    37921 cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc gatattggca
    37981 aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg
    38041 aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt tatgacttcg
    38101 cctgtctgaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgac cagtggggta
    38161 acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg tttggcctga
    38221 ctgttccgct gatcactaaa gcagatggca ccaaatttgg taaaactgaa ggcggcgcag
    38281 tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg atcaacactg
    38341 cggatgccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt gaagagatca
    38401 acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag tatgtactgg
    38461 cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca aaacgtatta
    38521 ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc gaacagctgg
    38581 cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg caggcactgg
    38641 tcgattctga actgcaacct tcccgtggtc aggcacgtaa aactatcgcc tccaatgcca
    38701 tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa gaagatcgtc
    38761 tgtttggtcg ttttacctta ctgcgtcgcg gtaaaaagaa ttactgtctg atttgctgga
    38821 aataatgcat taagtggaaa gggggagtga gaaatcactc cccctggttt ttatacaggg
    38881 aacatgatga aaaatattct cgctatccag tctcacgttg tttatggtca tgcgggtaac
    38941 agtgcggcag agtttccgat gcgccgcctg ggcgcgaacg tctggccgct gaacaccgtt
    39001 caattttcta atcacaccca atacggcaaa tggactggct gcgtgatgcc gcccagccat
    39061 ttaaccgaaa ttgtgcaagg cattgccgcc attgataaat tacacacctg tgatgccgta
    39121 ttaagtggct atctgggatc ggcggagcag ggtgaacata tcctcggtat cgtccgtcag
    39181 gtgaaagccg cgaatccgca ggcgaaatat ttttgcgatc cggtaatggg tcatccggaa
    39241 aaaggctgta tcgttgcacc gggtgtcgca gagtttcatg tgcggcacgg tttgcctgcc
    39301 agcgatatca ttgcgccaaa tctggttgag ctggaaatac tctgtgagca tgcggtaaat
    39361 aacgtcgaag aagcggttct ggcagcgcgc gaactcattg cgcaagggcc acaaattgtg
    39421 ttggttaaac acctggcgcg agctggctac agccgtgacc gttttgaaat gctgctggtc
    39481 accgccgatg aagcctggca tatcagccgt ccgctggtgg attttggtat gcgccagccg
    39541 gtaggtgttg gtgatgtgac gagcggttta ctgctggtga aactgcttca gggggcaacg
    39601 ctgcaggagg cgctggaaca tgtgaccgct gcagtctacg aaatcatggt gaccaccaaa
    39661 gcaatgcagg aatatgagct gcaagtggtg gctgctcagg atcgtattgc caaaccagaa
    39721 cattacttca gcgcaacaaa gctctgaaat atctcatttc ggcccacaac gacctgtggg
    39781 ccgatggcta ttactttaag ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag
    39841 ccatacgttg cataaatgct gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt
    39901 atgcccagcg cagaaccgta aacagatagg catcagcaat tgtaaatctt tgcccgcaga
    39961 tccagtgctc atccttcagt gcctcgttca catattgcag cttcttctcc agctgcgcgc
    40021 gaactgtcgg tttgtactct tccggtgtat cagggcgaaa cagaggtgtg aaacctttat
    40081 gcagctcggt ggcgatgtaa ttcagccatt cgatggtttt atagcgggaa atactgttta
    40141 ccggtgccag caactggcgg tcggggacgc tgtcggcaag atactgcata atcgctacgc
    40201 cttccgtcag caaagtaccg tcatccagca gcaatgcagg cacctgcccc ttagggttaa
    40261 cggcaaagta atcgtcaccg ttttcgagac gttttttcat taaatccaca ctgacgaggg
    40321 taaaatcctt tccgctctca cgcagggtga tatgggaagc gagagagcag gcacccggtt
    40381 tgtagaacaa tttcatcggt aactcctttt gtctgtggtt taaagtatgg tagtgcgttg
    40441 ctgaacaaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
    40501 tttacgctac ggctgctttc gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca
    40561 gtttcggcgc ggtcagcagc atcagtactg caataacggc agtagcgaca ccaatctgca
    40621 agaatacgcg accatagact tccagtgaca tcagcggatc ggtaacgtta tccggcacag
    40681 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
    40741 tacccataat gaagcccatc agacgctgcg gaacgagttg agcaaccatt gccagaccca
    40801 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta
    40861 cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
    40921 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct
    40981 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
    41041 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
    41101 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
    41161 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
    41221 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
    41281 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
    41341 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc
    41401 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
    41461 caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc
    41521 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
    41581 catagcatgt agaaagcaga gaagacgggt tagctttaaa caggccgtta ccgaccgcaa
    41641 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
    41701 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
    41761 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
    41821 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
    41881 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
    41941 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
    42001 acacttttac ctcttttttt acatcccata ttaacggggg tgctttctcg cgttatgtat
    42061 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
    42121 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
    42181 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaacg gcagattttt
    42241 tcactgcact gagagggggg tgatggctta acggcgatat gttctgctat ttagcgtttt
    42301 tactagtgag atagtccagt ttctgaaaaa tagccagtgt aatgttttgt aggtcaatat
    42361 ttgttgcgtg tgtaaattta aatgatttta acctgattct tgcaatgttt ttacattgcg
    42421 acagcatcgt gatcttgatc acgaatgaat agaaaatttc gctattaaaa aaacaattaa
    42481 cctgcatttt tggttatccg caaacggatt acctgatgat aacgctaagc aatggcatca
    42541 ttgacgttaa aaagatgttt taaaagagga taaagaaagg ttatcaatgg ggtaatcggt
    42601 gttacccctt ttcttcagat gtcaactttc tctttgtatt cacaaagatc ttcaataata
    42661 caagagccac agcggggctt gcgggcaatg caggtataac gcccgtgcag gatcaaccaa
    42721 tggtggcagt cgactttaaa ctctgctgga accactttca gtagcttttc ttctacctgt
    42781 tcgacgtttt tccccggcgc aaattgagta cgattacaaa cgcggaaaat gtgcgtgtcg
    42841 acagcaatag tcggccagcc gaatgcagtg tttaatacga cgttggctgt tttacgacct
    42901 acgccgggca gggcttcaag cgcagcacga tcttccggaa cctcgccatt atgctgctcc
    42961 agcaagatac ggcaggtttt gatgatattt tctgctttgc tgttataaag cccaatcgtt
    43021 ttgatatagg ttttcacccc ttcaacgccc agttcaagca tcgctgcagg cgtattcgcc
    43081 accgggtaga gtttcgccgt cgccttatta acactgacat cggtcgcctg agcggaaagc
    43141 agtacggcaa tcagcaattc aaaaggcgaa ctgaaattaa gctcggtggt gggatgagga
    43201 ttgttctcac gcaggcgagt gaggatctcc aggcgttttg ctttattcat cagacattcc
    43261 ctgtttcacc gtttggcaat gcacgttctg cagctgcttc agcacggcgc tttttcattc
    43321 tttcatcaat caggtatttt cctgccagca tcagtcccag gccaataaat gcacctggtg
    43381 gcagcatcgc cagcaggaaa ggggagtcgg tgtggaaaat ctccacgcgt aatacttttg
    43441 cccagctacc taacagcgca tctgcaccgt caaacaatgt gccattgccg ataatttcgc
    43501 gtagtgaacc cagcacgaac atggcgcagg ttgcgcccat accaattgaa aagccgtcca
    43561 gtgccgaaag cgccggacct tttttggcgg cgaaggcttc agcgcggccc acaacgatac
    43621 agttagtgac aatcagcgga ataaaaatcc ctaatgattg atacaggcca aaggcgtagg
    43681 cgttgatcag catctgtaca gcgctgacca ccgaggcgat gatcatcacg taaatgggaa
    43741 tgcggatctc ggctggcgtc cagtgacgca gcgtcgaaat ggtcaggttg gtcagcgtca
    43801 gtaccagcgt agtcgcaagt cctaaaccca gagcgttagt ggcagtggac gtgaccgcca
    43861 acagaggaca aaggccgagc aactggacca gcgcagagtt gtttttccac aacccctgaa
    43921 caataacgtc tttaatttcg ctcacgcttt attctccaca ggcaggaagt tgagaaagtt
    43981 gtgccggtaa cgtctgagcg tacaatccgg cgcgttttac cgcattaacc accgcgcggg
    44041 gagtaatcgt cgcgccggtg aactggtcga aatcaccacc atctttcttc accgcccagt
    44101 gcgcatcatc tgcaccactg atttttttac ccgcaaaatg ggtgatccag tcagaaaggc
    44161 gcagttcgat tttatcgcca agccctggcg tttcgtggtg ctctgtcacg cgcgtgccaa
    44221 gtaccgtgcc gttaaaatcg gctcccacca gcagctgaat cgcaccggaa tagccatctg
    44281 gcgcggttgc ttccagaacg gcggctaccg gtttgtcatc ctgtttggcg atgtaaaccc
    44341 gatgctcacc tttacctaac tctggcgcag ttaccagata gcaactctgt gccagcgcat
    44401 tgttatagcg ttcggctggc agcacctgat caaataacgc cttttgttgc agactggcct
    44461 gttcagcaat cgtcgttttg gtcatctggt tgatggccgc agttaaccct gttgaacccg
    44521 ctgcaaatag cgccaacgta atgccgtgtt ttcggatagt tttcagcatg gtttaccctt
    44581 tgcgatggcc gtagacgcgc ggacgcgtgt agtaatcgat cagaggaacc gtgatgttcg
    44641 ccagcaggac ggcaaaagcc acgccgtcag gatagccgcc gaaactgcgg atcaaccaga
    44701 ctaataagcc cgcaagcgcg ccgaaaataa gacgaccacg attggtcgta gaagcggtaa
    44761 ccgggtcagt caaaataaag aatgcgccga gcatggtcgc tccagacagc agatgaattt
    44821 gcggtgctgc cagtgtttct ggtgagaaca accagcccaa cattgcgcat aacgccagcg
    44881 ttactaagaa gctgagggga atatgccagc gaatcgcttt ctgccatagc aaccatacgc
    44941 cgccagccag ccaggcgaga tttacccatt gccaaccagc gcccgccaga ataccgctgt
    45001 agatcggata ttgcataatc tgttcaaccg aatgaccggc acggacagag gttttaaatg
    45061 tatccagcgg tgtcgcctga ctaatgccat caatacctaa gcgtagtgtg ttcatatcac
    45121 caccactggc ggtatgaccg ctaaaaataa cctggatggc gtcgataaaa ccagggatgt
    45181 tgaccgcaat ttcatgtggc ggtaaccagc tggtcatctg cacggggaag gagatcagta
    45241 agaccacata accaatcatt gccggattaa acgggttttg tcccagaccg ccatacaact
    45301 gtttagcgat gatcaccgca aacaccgtac ccagcacgac catccaccat ggcgcgaggg
    45361 ggggaatact taccgccagc aataagcctg tcagcaatgc tgagttatct ttcaacgttg
    45421 cggctaccga ctgcttgcgt agtttgagta cgagagcttc ggctaacaga gcactaaccg
    45481 atgccaacag gatctgaacg agagtacccc aaccaaaaaa ccacagttgc gctgcgattc
    45541 ctggcacggc tgcgagcaac accaacagca taatgcgcga tgtctggcgc tggttatggg
    45601 tataagggga gctagctatt ctgaatacca tttagtcctc gtttacaacc ttctgctggg
    45661 cggctttttt ggcctgagcg cgggcaatag ccgcggcaac tgccgctttg cgcggatcaa
    45721 cctgttcttc tggtaccgca ttagcctgtt gctgttccag cttgcgtgct ttggcgcgtg
    45781 caatagtcgc ttcgacggca gctttgcgcg gatcaacctg ttcttctggc tccgcattag
    45841 ccggttgctg ttcccgtttg cgcgctttgg ctcgggcaat agcggcttcg acggcggctt
    45901 tgcgcggatc gacctgttgt tctggctcgg cattagcctg ttgctgttcc agcttgcgtg
    45961 ctttggcacg ggcaatagcg gcttcgacgg cggctttgcg cggatcgacc tgttcttctg
    46021 gttccgcatt agcctgttgc tgttccagct tgcgcgcttt ggcacgggca atagcggctt
    46081 cgacggcggc tttgcgcgga tcgacctgtt gttctggttc cgcattagcc tgttgctgtt
    46141 ccagcttgcg cgctttggcg cgggcgatag ctgcttcaac ggcagtttta cgtggatcag
    46201 caacggttgc tgcgtcgtta gtttgctgca gttctgcctg tttcgctctg gcttgcgctt
    46261 tacgggcttc ccgtgctgca ataattgcac tgttatccgg gcgttcgccc gctttaatca
    46321 caataggctg tgtagcctgg gcctgtttct ctttcacccg cgccagagcg gtagcaatcg
    46381 catctttatc tttggctgca ggttgaacgg ctgcgctctt atgtcgttca aggcgagccg
    46441 ctttttcgcg ctccagacga gcctggcgcg cttcgaaacg cgctttggct tctgcggcgc
    46501 gcttttcttc ctgacgaata gccgcaattt cagctttttc ctgacggaaa tattgcacca
    46561 ggggaatatt gctcgggcaa acccacgcgc aagccccaca ttcaatgcaa tcagcaatgt
    46621 tatgcgtggt agctttatcg tgttgctgac ctttgctgaa ccagtacaac tgttgcggca
    46681 aaagatccgc agggcaggcg tcagcacagg cgctacaccg gatgcagctt tgttcttcct
    46741 gtggttcgcc aagttcattg gcagagggag ccaacagaca gttggtaatc tttacgaccg
    46801 ggacatccag ccatggcaag gtaaagccca ttagcgggcc acccataatc accatttgat
    46861 cggcagaggg gcagaatccg gcatcattca ataaatgacg cactggcgtc cccagccgtg
    46921 cccagacgtt gcccgggcga gcgattgctt cgccagtcag ggttacaaca cgctcggtaa
    46981 tcggctcgcc atcaataacg gcacgtttca ctgcataagc agtgccgacg ttttgcatta
    47041 atacgccgat atcggatgaa cgcccgccat gtggaacctg cttcccggtc agaatgtagg
    47101 ttaattgttt agcaccgcca gaaggatatt tggttggaat cacccgcaga gaaatatcgt
    47161 tagagtccgc cagcaccgcg cgcagcatgg aaatcgcctg cggtttgtta tcttcaatgc
    47221 cgataagaat ttcgcgtggc tgcagaatat gcgcaagaat gcgaataccc tctacgacct
    47281 gagccgcgca atcctgcatc aaacggtcat cggcggtaat gtacggctcg cactcagccg
    47341 cgttgataat caacgtttca atcttatctc cgccaccctg caatttaacg cctgtcggga
    47401 atcctgcacc gcccagcccg gcaacaccaa actgatgtat gcgctcgatt aactcttcgc
    47461 gactgcgagt gcgataatcg gcccagccgt cgcgcgggat ccagcagtct tcaccatcgg
    47521 catcaataat cacgcttaat tcagctaaag ctgaaggatg agccgtagag tggggcgcaa
    47581 tagccgtaac ggtacccgag gtgggcgcgt gaacaggcag cattttgccg cgaccacggg
    47641 taagcggctg gccgcgcaat actttatcgc cgacgctaac gcacaactca ccttcagcgc
    47701 caatatgctg tttcagtgga ataacaaaac gctgcgccag gggtacctgg cgcaggggtg
    47761 taccgttgga ctgggttttc atctccggtg gatggatgcc gccgttgaaa tcccagattt
    47821 tattttttct gaatgcagag aataacttaa gcatggtgtt ccacgggaat gatacgcacg
    47881 ggaatggtgt tcagatccca tttccaggag tcaggtgttt ctgcgaccgg ttgcaacgag
    47941 atgcagtgcg tcgggcacgg atcaacacat aaattgcagc ccgtacagag atcactcatt
    48001 accgtatgca tggcacgggt agcgccaacg atggcgtcta ccggacacgc ctgaatacat
    48061 ttagtgcagc caatacagtt attttcatca ataaccgcca ccatccgcgc aggcgttatc
    48121 tcttgcgctt cgccatccag cggctgcggc tcgacattaa gcaactcggc aatttttagc
    48181 atcacagctt cgccacctgg ggcgcaacgg ttgatttttt caccgttaca gctgatggct
    48241 tccgcgtagg ggcgacagcc gggataaccg cactgaccac actggctctg cggtaagatt
    48301 tcgtcaattt tctcaacgac cggatcgtct tccaccgcaa aacggcggga ggcataaccc
    48361 agaatggcgc caaacgccag gcccagcagg ctcacggcgg caacggcaat ccagatagca
    48421 ttcattacaa cttcaccaaa ccactaaagc ccataaaggc cagagacata agacctgcgg
    48481 taattaacgc aatggcatta ccgcgaaaag gtgccgggac atcagccaca gcaaggcgtt
    48541 cgcggatggc ggcgaagagc accatcacca gcgagaaacc gacagcggcg gaaaaaccgt
    48601 acagcgccga ctgcaagaaa ttgtgcccga gattgatatt cagcaacgcc acgccgagca
    48661 ctgcacagtt ggtggtgata agcggcaaaa aaatccccag cagacggtaa agcaccgggc
    48721 tggttttgcg caccaccatc tcggtgaact gcacgaccac agcaatcacc agaataaatg
    48781 ccagggtgcg caggtaaatc agattaagtg ggatcaaaat ccacgtatcg ataagccagg
    48841 cgcaaataga cgccagcgtc atcacaaacg ttgttgccag ccccatgccc atcgcggttt
    48901 ccagtttttt ggaaaccccc ataaacggac agagaccgag aaacttgacc agtacaaagt
    48961 tattgaccag tacagttccg acaaacagta acaggtagtc agtcattttt cggcctgaaa
    49021 taaaaaagcc gcctattatc cgttaatcca gagcaggcga caacaggtta actgtaaggt
    49081 tattacggat tcacaaacgt cgcttttacc cgcgacgagc gtttgaaata ggggacgatc
    49141 aatgcggttg ccaacaaagt aaacagcaac tggcgaacgg ctatgccatc ttccacgggt
    49201 gaaaaagcga atgcttttac tgccagcaat acggaaataa gcagccagat aatatagtgt
    49261 ttaggaacac aacgccgacg tttaaagaaa gcgatggtca accacagtgt gtaataccac
    49321 aaggcgatag cggtaataaa agaaacgccc cataagattt gtgtcgtcag tgcttgtccg
    49381 ccgagcgttt gaaatgtttg cggagaagat aacgcagcgg tatacagcaa caacgccagc
    49441 gtcgtactca acagagcgac taaaagccag gccaacgggc caagcaacca gcctccaata
    49501 cgttgtggcg ttgtggtggt cataggttct ccagaatagc gacgcaaaca ggcaaatcta
    49561 attttagggg cgttattata aggcttttat aaccgatcgc tactttcttt attggacata
    49621 gtgccagacg gacttcggta cctggccgag gtcaaacaaa cgcccgccag aaaccagctc
    49681 tgcgcgacgg tgatcggcag cacgatacat attgatcagt tcctgatcgt ccgtcagggt
    49741 gtaattaaga tggtcgtaga gtttttccag actttcgagt gaactgattt tgcgaaattt
    49801 taataagtag tcctgaacag tcataaaagg tccataaaat aattaatacc aaaggggtag
    49861 gtattattag tgatgataaa atttcaccaa aagtgaacgc ttgtgttatt acaataataa
    49921 gaatgcgctg atggcaaata ttcagggatg aaaaactgct ctgaaaagag taacgcgctt
    49981 tactatccct gacaatcacc aacaacatcg aacaagataa taaattcctg gtttaatatc
    50041 cgacaagtga aaacatgcac ccggacgggc agcatgtcgc tccacaagtg cagagcttac
    50101 ttgtgttgta ccgaagcact ctgttcaggt ggctgatagt tgtcaatgtg actcgccacg
    50161 ccaagaagaa tgactgaaac gacaagaacg atccaacctg ttaattcaat aagacgattc
    50221 attacagccc acatctctct tgattgatcc attaacttca gggggtaaat gttacttagc
    50281 aataatagct ccagcaagat ttttactgag gtttttgcga tatttagctt ttgtcgttgg
    50341 aaaattcgct atttttttga cttaagttaa acaacatccc ttattgctgg caggttatta
    50401 aactgttgag cgtgggtaaa ggatagtgtc aaatagccat catacttcaa tgagaggcaa
    50461 tgacatgagc gacaacatcc gtgttgggtt gattgggtat ggttatgcga gcaaaacctt
    50521 ccatgcgccc ctgattgcgg gcacgcccgg gctggaactg gcggtaatct ccagcagtga
    50581 tgaaacaaaa gtaaaagccg actggccaac ggttacggtt gtctctgagc cgaagcatct
    50641 gtttaacgat cccaacatag acctgattgt cattcctaca cccaacgata cccatttccc
    50701 gttagccaaa gcggcgcttg aggcgggtaa acatgtggtc gttgataaac cctttaccgt
    50761 gacactgtca caagcgcgag agctggatgc gctggcaaaa agcctggggc gtgtgctgtc
    50821 tgtattccat aaccgtcgct gggatagcga tttcttgacg ctaaaaggtt tactcgcgga
    50881 aggcgtgctg ggtgaagttg cttactttga gtctcatttt gaccgcttcc gtccgcaggt
    50941 gcgcgatcgt tggcgtgaac agggcggccc aggcagcggt atctggtacg atttagcacc
    51001 acatcttctt gatcaggcca ttacgctttt tggtttaccg gtcagcatga cggtagattt
    51061 ggcacagtta cggcccggag cgcagtcgac cgattatttc cacgccatct tgtcctatcc
    51121 acagcggcga gtcattttac acggtaccat gctggcagcc gctgagtcag cacggtatat
    51181 cgtgcatgga tcccgaggca gttatgtgaa atatggcctc gatccacagg aagaacgtct
    51241 gaaaaatggc gagcgtctac cgcaggaaga ctggggctac gatatgcgtg atggcgtact
    51301 tacccgcgtg gaaggtgagg aacgtgtcga agaaacgctg ttgacggtgc ctgggaatta
    51361 tccggcttac tatgcggcta ttcgtgatgc gttaaatggc gatggtgaaa atccggttcc
    51421 ggcaagccag gcaatccagg taatggagtt gattgagctg ggcatcgaat ccgccaaaca
    51481 tcgcgcgact ttgtgccttg catgaaaaat ggatggtgca tttgcaccat ccattttgtt
    51541 acttcgcggc gactttttct cgcagtgcgc gtttttcctc agcgctgagg aaagccattt
    51601 ccagaccatt aatctgtgcc tggcggattt gctcgcggga taacccagca gctggcgcgg
    51661 caacggtata ttcgtgaatg atatccactc cctgtacgcc gggatcgtca gtgttaatgc
    51721 tggcacgaat gccatgctca aggaacgttt tcagcggatg tgcagccagc tctgctacgg
    51781 tgctggtctg aatattggag gtcagacagg attcaatacc aatttgttgc tcggcgagaa
    51841 aatccatcag cgcccgatct tcaatggctt ttacgccatg tccaatacgc tccgcaccca
    51901 gttcacgaat cgcctgccag atgctttccg gcccggcagc ttcgcctgca tggacggtaa
    51961 tatgccagcc cgcatcacgc gcgcggttga agtgagaaag gaacagactt cccgggaaac
    52021 caagttcatc accggctaaa tcaagtgcgg taatctggtc acggtgggct aaaaaggcct
    52081 ccagctcttg ctgacaggcg gcttcgccga aggtccggct cataatgccg ataagcttcg
    52141 cctgcacacc aaaggtgcgg caaccttcac gtacgccatc gatcaccgct tcgacaacac
    52201 ccgctacagg cagctgatgt gccattgcca tgtagcctgg tgaaaaacgc agctcgacat
    52261 agtgcaggcc gtgacgggct gcatcttcaa tgttttcaaa tgccacgcgg cgacaggcat
    52321 caagagaggc gagaacttta acgccccagt caagtttggt cagaaagctc accagatcgg
    52381 gttcgttggc aatgacctga acgtggggaa tcagtgtttc cagggattgt gcaggaagcg
    52441 agatattata ctggcggcca agttcaagaa tggtctgggg acgaatgttg ccatcaaggt
    52501 ggcgatggat atcagttaat ggcagggtgg tatcaatcat ggtcgcactc tttttctgga
    52561 tataaaagtg cgcaccatta taaaaacaaa gcagggtaaa aatccacgat tgcgcaacga
    52621 ttttttacgt tgcgcaattg gggttaacga acagcgcgga tggcgttaat taatccagcc
    52681 acaccttttt ccagtttcga acgtgggcag ccggcattga gacggacaaa accacgacct
    52741 tcttcaccgt aggtataccc cggcatgatc gcgacttttt cttgttcgat aagtgctttt
    52801 tgcaacgcgt tgtcgtcaat attcaacgga cgtaaatcaa gccatgccag ataagtggat
    52861 tgtgggatct gccagttgag ttcaggaaac gcggcgttca ttttatctgc gatatacgtc
    52921 aggttatctt tcagatagat gcgtaaggca tccagccacg gcgcgccttg ctgataggcg
    52981 gcgatatggg cagttaacgc cagtaccgaa ggggaagaaa gcccatcacg gcctttcagt
    53041 gccgataaat aggcatcgcg gctactgcta ttttctataa tcccgtaagc accggtcagg
    53101 gcgggaatat tgaaactttt cgagcccgac gttagcaacg cccagtctcc gcgagccaca
    53161 ttactccagg gaatatgcgg ctgctcgccc caaaccatat ccatatggat ttcatcggaa
    53221 ataacccgca caccatgacg ctcgcacagg tcagccatga tctccagctc atcgcacgtc
    53281 cacactttcc cggtaggatt ctgtgggcta cacaggagca taattttaca ttctggtttc
    53341 gccaacacgg cttccaactt gcccatatcg caaaaccaac catcagcctg cttctctaaa
    53401 gcaacgggca ttactgtgcg ctggttacct tcaatggcct tgtaaaatgc gtcataggcg
    53461 ggtgtgtgga tcaccacgcc ttcacctgtt tcagaccact gacgaatcag ttctgaaacc
    53521 atatagatga cagaagggcc atacaccacc gtctgagaat cgatggcggt gtaatgctgg
    53581 gtggaaaacc agtgggcaat agccgcgaga aactcatcgt ttttccagcg gctgtagcca
    53641 aatacgccgt gcatcaggcg ctgattcagc gcctcgataa tgcagggggc agtggcaaaa
    53701 tccatgtctg aaatcgtgaa cggtaacagg tcagcagtgc cgaaacggtc agcgacataa
    53761 tcccactgtg tacaccatgt gccatgacga tccacgacct ttgaaaaatc gaacatatct
    53821 tatccttatg cctggacagt atgcatcaga ccggccattt catcttttac tgactgaact
    53881 tgtggcccga taacaacctg caggttatgt tgattaagtt gtactacgcc aattgcccga
    53941 ttgtccttca gtgcctgcac attaacaagc gacatatctt tcacagacaa acgcagacgg
    54001 gtaatgcagt tatcgaggct gacaatattg tcggcaccgc ctaatgcttc gaggattgca
    54061 ggaacgttgt aacctgattt acccggcgca ccggcaacgg ctttttcgat tgagctggca
    54121 acttcgctat cgcgccccgg ggttttcaga ttgaagcggg tgatagcgaa acggaagatg
    54181 acgtagtaaa cgacaaacca gattgccgcc accactggca ccatgtacca cttggttgac
    54241 agaccatgca aaataccgaa caccacgaag tcgatgatat tgccgtcggt attaccgatg
    54301 gtgacgccga gcacagacat gacggtgaag ccgaggccgg ttaacagcgc gtggatgaca
    54361 tacagaactg gcgctacgaa caggaacagg aattccagcg gttcggtagt gccgccaacg
    54421 acgcaggcga tcaggccaga aatcagcaga cctttaattt tatggcgatt ttccgggcgc
    54481 gcgcagtgat acatagctaa cgctgcacct ggcagaccgc cgagaaacgc aggcatttta
    54541 ccttgcgaaa ggaaacgcgt ggcgctttca gaaaaaccgt gagtggtcgg gcaactcaat
    54601 tgcgcctgga agatggtcag tgcgccgctg acggtttgac cgcagacttc ctgcgtgccg
    54661 cctgcgtcgg taaagcgaat taatgccacc agaatgtgat gcagaccaaa cggcaacagc
    54721 agacgttcac cggtaccaaa cagcatcggt ccgaaatcac ccgcgctgtt tatcatatgg
    54781 cccaagccgc taatacccat ggcgaaaatc ggccagacta atggaatcac caggccgaca
    54841 aggcccatca ccagcgagga gataattggt acgaagcgcg taccgccgaa gaatgccagc
    54901 gcatccggca ggcggatatt atgaaaacgc tcatgcagca tccagacgat aataccggcg
    54961 atcaccgcac cgaggatccc ggtatcgatc gactggatcc caagaatgct ctggatgtta
    55021 ttggctttca gaaccgcggc atccgtggtt ggcagaatgc ctttattggt caaccagaag
    55081 tttaccgcga ggttcattac cgcataaccg atgaagccag cgaatgccgc tacgccttta
    55141 ttttcgcgtg ccaggcccag cgggatggcg atacagaaca tgacaggcag gaaactaaaa
    55201 gcaaacgagc caatcttact catccaggta aagatagcct gcaacacggg gttgcccagg
    55261 accgggatca gggttatgac atcatggctg ctaagagaac taccaatgcc gagcataatg
    55321 ccgcagaacg acaataatgc cacgggtaac atgaaggttt tgcctaactg ctggaagaac
    55381 tcccacagcg tgactttcgg tgctgttttc gccgtcataa aacgactcct cgtagagaac
    55441 aatctggatt cagtaaattg cgcgagaaga taaaacgttt tatcaaattt tagtgaggca
    55501 taaatcacat tacgcaacga taatagcggg tataagataa ataaaaggta aaacgtttta
    55561 tctgtcacat aatcagggag taggtcatct gcatggctac cgccaaaaaa ataaccattc
    55621 atgatgttgc gctggctgcg ggcgtgtcgg taagtaccgt ttcgctggtg cttagtggca
    55681 aagggcgaat ctctaccgcc acaggagaac gcgttaacgc cgccattgaa gagctgggat
    55741 ttgtgcgcaa tcgccaggcg tcggcgctgc gcggcgggca aagcggcgtc attggtttga
    55801 tcgtccgtga tttatctgcg ccgttttacg ccgaattgac ggccggattg acggaagctc
    55861 tggaagcgca gggacggatg gtttttttgc ttcacggcgg taaagacggt gagcagctgg
    55921 cacagcggtt ttcactgtta ctgaatcagg gtgtcgatgg tgtggtaatt gccggagctg
    55981 caggaagtag cgatgacctg cgacggatgg cagaagaaaa agctatcccg gtgattttcg
    56041 cttcccgtgc cagttatctt gatgatgttg atacggttcg cccggacaac atgcaggctg
    56101 cacagttgtt gacggagcat ctcattcgca atgggcatca gcggatcgcc tggctgggag
    56161 ggcaaagttc ctcattaacc cgtgcagaac gggttggggg ctattgtgca actctactaa
    56221 aatttggcct gccgtttcat agcgattggg tgttggagtg cacttccagc cagaagcaag
    56281 ccgcggaagc tatcacggcg cttttacgtc ataacccgac catcagtgcc gtggtttgct
    56341 ataacgaaac tattgcgatg ggggcatggt ttggtttgct caaagcaggg cggcaaagcg
    56401 gggaaagcgg agtcgatcgt tactttgagc aacaggtttc gctggcggca tttaccgatg
    56461 cgacaccaac cacacttgat gatatacctg ttacctgggc cagcacgcca gcgcgggaac
    56521 ttggtatcac acttgcggat cgcatgatgc aaaaaatcac ccatgaagag acgcattcac
    56581 gcaatcttat tattcccgcc cggctcattg cagcgaaata atcctctctt tatctgctat
    56641 acctggtagt gtcctttcct caaggttaat gataaaaaat gtggcacaca ggcggtgcct
    56701 tttattttta ctgaagttca caggaggttt atgtgtttaa ttctgacaac ctgagactcg
    56761 acggaaaatg cgccatcatc acaggtgcgg gtgcaggtat tggtaaagaa atcgccatta
    56821 cattcgcgac agctggcgca tctgtggtgg tcagtgatat taacgccgac gcagctaacc
    56881 atgttgtaga cgaaattcaa caactgggtg gtcaggcatt tgcctgccgt tgtgatatta
    56941 cttccgaaca ggaactctct gcactggcag actttgctat cagtaagctg ggtaaagttg
    57001 atattctggt taacaacgcc ggtggcggtg gacctaaacc gtttgatatg ccaatggcgg
    57061 attttcgccg tgcttatgaa ctgaatgtgt tttctttttt ccatctgtca caacttgttg
    57121 cgccagaaat ggaaaaaaat ggcggtggcg ttattctgac catcacttct atggcggcag
    57181 aaaataaaaa tataaacatg acttcctatg catcat
//
LOCUS       DBBGLQ010000027        54779 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.027, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000027 DBBGLQ010000000
VERSION     DBBGLQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.027"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..1070
                     /gene="bcsE"
                     /locus_tag="ACIYSB_002070"
     CDS             <1..1070
                     /gene="bcsE"
                     /locus_tag="ACIYSB_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HGC5429135.1"
                     /translation="FGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLV
                     QSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSL
                     QQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPL
                     SRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLP
                     AHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALN
                     HIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDG
                     RHWRRIPQPMRLLDDAVERSS"
     gene            1067..1258
                     /gene="bcsF"
                     /locus_tag="ACIYSB_002071"
     CDS             1067..1258
                     /gene="bcsF"
                     /locus_tag="ACIYSB_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGC5429136.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            1255..2934
                     /gene="bcsG"
                     /locus_tag="ACIYSB_002072"
     CDS             1255..2934
                     /gene="bcsG"
                     /locus_tag="ACIYSB_002072"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGC5429137.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPLPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGNMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIEQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(3021..3128)
                     /gene="ldrD"
                     /locus_tag="ACIYSB_002073"
     CDS             complement(3021..3128)
                     /gene="ldrD"
                     /locus_tag="ACIYSB_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HGC5429138.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            3604..4875
                     /gene="yhjV"
                     /locus_tag="ACIYSB_002074"
     CDS             3604..4875
                     /gene="yhjV"
                     /locus_tag="ACIYSB_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGC5429139.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(4905..5909)
                     /gene="dppF"
                     /locus_tag="ACIYSB_002075"
     CDS             complement(4905..5909)
                     /gene="dppF"
                     /locus_tag="ACIYSB_002075"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGC5429140.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(5906..6889)
                     /gene="dppD"
                     /locus_tag="ACIYSB_002076"
     CDS             complement(5906..6889)
                     /gene="dppD"
                     /locus_tag="ACIYSB_002076"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGC5429141.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(6900..7802)
                     /gene="dppC"
                     /locus_tag="ACIYSB_002077"
     CDS             complement(6900..7802)
                     /gene="dppC"
                     /locus_tag="ACIYSB_002077"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGC5429142.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(7812..8831)
                     /gene="dppB"
                     /locus_tag="ACIYSB_002078"
     CDS             complement(7812..8831)
                     /gene="dppB"
                     /locus_tag="ACIYSB_002078"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGC5429143.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            8995..9105
                     /locus_tag="ACIYSB_002079"
                     /pseudo
     CDS             8995..9105
                     /locus_tag="ACIYSB_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9139..10746)
                     /gene="dppA"
                     /locus_tag="ACIYSB_002080"
     CDS             complement(9139..10746)
                     /gene="dppA"
                     /locus_tag="ACIYSB_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGC5429144.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(11489..11565)
                     /locus_tag="ACIYSB_002081"
     tRNA            complement(11489..11565)
                     /locus_tag="ACIYSB_002081"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11529..11531),aa:Pro,seq:cgg)
     gene            complement(11657..13348)
                     /gene="eptB"
                     /locus_tag="ACIYSB_002082"
     CDS             complement(11657..13348)
                     /gene="eptB"
                     /locus_tag="ACIYSB_002082"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGC5429145.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            complement(13672..14880)
                     /gene="yhjX"
                     /locus_tag="ACIYSB_002083"
     CDS             complement(13672..14880)
                     /gene="yhjX"
                     /locus_tag="ACIYSB_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5429146.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALLMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            complement(15109..15807)
                     /gene="yhjY"
                     /locus_tag="ACIYSB_002084"
     CDS             complement(15109..15807)
                     /gene="yhjY"
                     /locus_tag="ACIYSB_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGC5429147.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGLS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            15965..16528
                     /gene="tag"
                     /locus_tag="ACIYSB_002085"
     CDS             15965..16528
                     /gene="tag"
                     /locus_tag="ACIYSB_002085"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGC5429148.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFVDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKELKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            16525..16965
                     /gene="yiaC"
                     /locus_tag="ACIYSB_002086"
     CDS             16525..16965
                     /gene="yiaC"
                     /locus_tag="ACIYSB_002086"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGC5429149.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(16934..19267)
                     /gene="bisC"
                     /locus_tag="ACIYSB_002087"
     CDS             complement(16934..19267)
                     /gene="bisC"
                     /locus_tag="ACIYSB_002087"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGC5429150.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGCHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQERGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            19420..20079
                     /gene="yiaD"
                     /locus_tag="ACIYSB_002088"
     CDS             19420..20079
                     /gene="yiaD"
                     /locus_tag="ACIYSB_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGC5429151.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTSVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            20183..21157
                     /gene="ghrB"
                     /locus_tag="ACIYSB_002089"
     CDS             20183..21157
                     /gene="ghrB"
                     /locus_tag="ACIYSB_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGC5429152.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVNAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(21207..21917)
                     /gene="yiaF"
                     /locus_tag="ACIYSB_002090"
     CDS             complement(21207..21917)
                     /gene="yiaF"
                     /locus_tag="ACIYSB_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGC5429153.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            22351..22641
                     /gene="yiaG"
                     /locus_tag="ACIYSB_002091"
     CDS             22351..22641
                     /gene="yiaG"
                     /locus_tag="ACIYSB_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGC5429154.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            22922..23134
                     /gene="cspA"
                     /locus_tag="ACIYSB_002092"
     CDS             22922..23134
                     /gene="cspA"
                     /locus_tag="ACIYSB_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGC5429155.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            23273..23344
                     /gene="ysaE"
                     /locus_tag="ACIYSB_002093"
     CDS             23273..23344
                     /gene="ysaE"
                     /locus_tag="ACIYSB_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HGC5429156.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFAG"
     gene            complement(23321..23473)
                     /gene="hokA"
                     /locus_tag="ACIYSB_002094"
     CDS             complement(23321..23473)
                     /gene="hokA"
                     /locus_tag="ACIYSB_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGC5429157.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            23553..24922
                     /locus_tag="ACIYSB_002095"
     CDS             join(23553..24033,24033..24922)
                     /locus_tag="ACIYSB_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HGC5429158.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            complement(25201..27270)
                     /gene="glyS"
                     /locus_tag="ACIYSB_002096"
     CDS             complement(25201..27270)
                     /gene="glyS"
                     /locus_tag="ACIYSB_002096"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGC5429159.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFTEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(27280..28191)
                     /gene="glyQ"
                     /locus_tag="ACIYSB_002097"
     CDS             complement(27280..28191)
                     /gene="glyQ"
                     /locus_tag="ACIYSB_002097"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGC5429160.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(28286..28585)
                     /gene="ysaB"
                     /locus_tag="ACIYSB_002098"
     CDS             complement(28286..28585)
                     /gene="ysaB"
                     /locus_tag="ACIYSB_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGC5429161.1"
                     /translation="MMMNAFFPAMALMVLVGCSIPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            28760..29755
                     /gene="wecH"
                     /locus_tag="ACIYSB_002099"
     CDS             28760..29755
                     /gene="wecH"
                     /locus_tag="ACIYSB_002099"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGC5429162.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(29797..30234)
                     /gene="yiaA"
                     /locus_tag="ACIYSB_002100"
     CDS             complement(29797..30234)
                     /gene="yiaA"
                     /locus_tag="ACIYSB_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGC5429163.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(30280..30621)
                     /gene="yiaB"
                     /locus_tag="ACIYSB_002101"
     CDS             complement(30280..30621)
                     /gene="yiaB"
                     /locus_tag="ACIYSB_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGC5429164.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFTHICQLVALITIGLLFIGVLNAPINTYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(30790..32244)
                     /gene="xylB"
                     /locus_tag="ACIYSB_002102"
     CDS             complement(30790..32244)
                     /gene="xylB"
                     /locus_tag="ACIYSB_002102"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGC5429165.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(32316..33638)
                     /gene="xylA"
                     /locus_tag="ACIYSB_002103"
     CDS             complement(32316..33638)
                     /gene="xylA"
                     /locus_tag="ACIYSB_002103"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGC5429166.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHHLSPVHQSGRQEQLENLVNHYLFDK"
     gene            34004..34996
                     /gene="xylF"
                     /locus_tag="ACIYSB_002104"
     CDS             34004..34996
                     /gene="xylF"
                     /locus_tag="ACIYSB_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGC5429167.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            35074..36615
                     /gene="xylG"
                     /locus_tag="ACIYSB_002105"
     CDS             35074..36615
                     /gene="xylG"
                     /locus_tag="ACIYSB_002105"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGC5429168.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            36593..37774
                     /gene="xylH"
                     /locus_tag="ACIYSB_002106"
     CDS             36593..37774
                     /gene="xylH"
                     /locus_tag="ACIYSB_002106"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGC5429169.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            37852..39030
                     /gene="xylR"
                     /locus_tag="ACIYSB_002107"
     CDS             37852..39030
                     /gene="xylR"
                     /locus_tag="ACIYSB_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGC5429170.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(39226..40050)
                     /gene="bax"
                     /locus_tag="ACIYSB_002108"
     CDS             complement(39226..40050)
                     /gene="bax"
                     /locus_tag="ACIYSB_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGC5429171.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITVERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            40370..42400
                     /gene="malS"
                     /locus_tag="ACIYSB_002109"
     CDS             40370..42400
                     /gene="malS"
                     /locus_tag="ACIYSB_002109"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGC5429172.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIM
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ"
     gene            42578..43831
                     /gene="avtA"
                     /locus_tag="ACIYSB_002110"
     CDS             42578..43831
                     /gene="avtA"
                     /locus_tag="ACIYSB_002110"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGC5429173.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(43982..44455)
                     /gene="ysaA"
                     /locus_tag="ACIYSB_002111"
     CDS             complement(43982..44455)
                     /gene="ysaA"
                     /locus_tag="ACIYSB_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGC5429174.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHHENQDCAALSPDEFISRI
                     RVIKDHCWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(44557..45405)
                     /gene="yiaJ"
                     /locus_tag="ACIYSB_002112"
     CDS             complement(44557..45405)
                     /gene="yiaJ"
                     /locus_tag="ACIYSB_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HGC5429175.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            45606..46604
                     /gene="yiaK"
                     /locus_tag="ACIYSB_002113"
     CDS             45606..46604
                     /gene="yiaK"
                     /locus_tag="ACIYSB_002113"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HGC5429176.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            46616..47083
                     /gene="yiaL"
                     /locus_tag="ACIYSB_002114"
     CDS             46616..47083
                     /gene="yiaL"
                     /locus_tag="ACIYSB_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGC5429177.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYTQIIDLTTREAVVNRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRNI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCIMQTASEIRKIVVKVALTALN"
     gene            47201..47674
                     /gene="yiaM"
                     /locus_tag="ACIYSB_002115"
     CDS             47201..47674
                     /gene="yiaM"
                     /locus_tag="ACIYSB_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HGC5429178.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            47677..48954
                     /gene="yiaN"
                     /locus_tag="ACIYSB_002116"
     CDS             47677..48954
                     /gene="yiaN"
                     /locus_tag="ACIYSB_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HGC5429179.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIGPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            48967..49953
                     /gene="yiaO"
                     /locus_tag="ACIYSB_002117"
     CDS             48967..49953
                     /gene="yiaO"
                     /locus_tag="ACIYSB_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HGC5429180.1"
                     /translation="MKLHSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKFLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            49957..51453
                     /gene="lyxK"
                     /locus_tag="ACIYSB_002118"
     CDS             49957..51453
                     /gene="lyxK"
                     /locus_tag="ACIYSB_002118"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HGC5429181.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDLRHPVRTLLPDMTAHQLYQKKYQRYQHLIAALQGFHARIKEHTL"
     gene            51450..52112
                     /gene="ulaD"
                     /locus_tag="ACIYSB_002119"
     CDS             51450..52112
                     /gene="ulaD"
                     /locus_tag="ACIYSB_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGC5429182.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            52105..52965
                     /gene="sgbU"
                     /locus_tag="ACIYSB_002120"
     CDS             52105..52965
                     /gene="sgbU"
                     /locus_tag="ACIYSB_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGC5429183.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGDDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            52959..53654
                     /gene="araD"
                     /locus_tag="ACIYSB_002121"
     CDS             52959..53654
                     /gene="araD"
                     /locus_tag="ACIYSB_002121"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGC5429184.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(53686..53787)
                     /locus_tag="ACIYSB_002122"
                     /pseudo
     CDS             complement(53686..53787)
                     /locus_tag="ACIYSB_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            53791..53979
                     /locus_tag="ACIYSB_002123"
                     /pseudo
     CDS             53791..53979
                     /locus_tag="ACIYSB_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(54001..54741)
                     /gene="yiaT"
                     /locus_tag="ACIYSB_002124"
     CDS             complement(54001..54741)
                     /gene="yiaT"
                     /locus_tag="ACIYSB_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGC5429185.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRSSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT        13589 a        14165 c        13681 g        13344 t
ORIGIN      
        1 tttttggtct tgccagtttg cgttttcagg gtgaccaaca tttgctggat attgccttct
       61 ggtgcaacga aaaaggggtc agcgcccgtc agcagcttag cgttcagcaa caaaatggta
      121 tctggacatt agttcaaagc gaagaggcgg agatccaacc acgcagcgac gaaaaacgca
      181 ttctgagtaa tgttgctgta ctggaaggtg cgccgccgct atcggaacac tggcaactgt
      241 tcaacaataa cgaagtcctg ttcaatgaag cccgtaccgc tcaggcggcg acggtggtct
      301 tttctttaca gcaaaatgcg caaatcgagc cactggcccg cagcattcat accctgcgtc
      361 gccagcgcgg tagtgcgatg aaaatcctcg tgcgggaaaa taccgctagc ctgcgcgcca
      421 ccgatgaacg tttgttattg gcctgcggtg caaatatggt tattccgtgg aatgcgccac
      481 tctcccgttg tctgacgatg atcgaaagcg tgcaagggca gaagtttagt cgctatgtgc
      541 cggaagatat cactaccttg ctgtcaatga cccagccgct caaactgcgt ggtttccaga
      601 agtgggatgt gttctgtaat gccgtcaaca acatgatgaa taaccctcta ttacctgccc
      661 acggtaaagg cgttctggtt gccctacgtc cggtaccggg tatccgcgtt gaacaagccc
      721 tgacgctgtg tcgccctaac cgtaccggcg atatcatgac cattggcggt aatcggctgg
      781 tgctgtttct ctcattctgt cggattaacg atctggatac cgcgttgaat catattttcc
      841 cattgcctac tggcgacatt ttctcaaacc gtatggtctg gtttgaagat gatcaaatca
      901 gtgccgagct ggtgcagatg cgcttgcttg ccccagaaca atggggcatg ccgctgcctt
      961 taacgcaaag ttctaaaccg gtcatcaatg ccgagcacga tggtcgccac tggcgacgaa
     1021 taccacaacc catgcgactg ttagatgatg ctgtggagcg ctcatcatga tgaccatcag
     1081 cgatatcatt gaaattattg tcgtttgcgc actgatattt ttcccgctgg gctatctggc
     1141 gcggcactct ttgcgacgca ttcgcgacac cttacgtttg ttctttgcta aacctcgtta
     1201 tgttaaaccg gccgggacgt tacgccgcac ggaaaaagcc agggcaacca aaaaatgact
     1261 caatttacgc aaaataccgc catgccttct tccctctggc aatactggcg cggcctttcc
     1321 ggctggaact tctattttct ggttaagttc ggcctgttgt gggcgggata tcttaacttc
     1381 catccgctcc tcaatttggt gtttgccgcg tttctgctga tgccccttcc gcgctacagc
     1441 ctgcatcgct tgcgccactg gattgccctg ccgatcggct ttgctttgtt ctggcatgac
     1501 acctggttgc ctggcccgga aagcataatg agccagggtt cgcaggtggc ggggttcagt
     1561 accgattatt taatcgacct tgtcacacgc tttattaact ggcagatgat tggggccatt
     1621 tttgttttat tagtggcctg gttattcctg tcacaatgga ttcgcattac cgtttttgtg
     1681 gttgccatac tgctatggct gaacgtactt accctggcgg gaccaagttt ctccttgtgg
     1741 ccagccggac aaccgacgac cactgtaaca acgacgggtg gtaacgcagc ggcaaccgtt
     1801 gcggcgacgg gtggcgcacc ggtagtgggt aatatgcccg cacaaactgc accgccaaca
     1861 acggcgaacc ttaacgcctg gctgaataat ttctataacg cggaggcgaa acgtaaatcg
     1921 accttcccgt cttcgctgcc cgctgatgct cagccatttg aactactggt gattaacatc
     1981 tgttcgcttt cctggtcgga tatagaagcc gccgggttga tgtcgcatcc actgtggtcg
     2041 catttcgata ttgagttcaa gaactttaac tccgccacct cctacagtgg cccggcggcg
     2101 atccgtttac tgcgcgccag ctgcgggcag acttcgcaca ctaatctgta tcaaccggca
     2161 aataacgact gctatctgtt tgataacctt tcgaaactgg gctttaccca gcacctgatg
     2221 atgggacata acggccagtt cggcggtttt ttgaaagaag ttcgcgaaaa tggcggcatg
     2281 cagagcgaat tgatggatca aacaaatctg ccggttattt tgctgggctt tgatggttcg
     2341 ccggtttatg acgataccgc tgtgcttaac cgctggctgg acgttaccga aaaagataaa
     2401 aacagccgta gtgccacgtt ctacaacacg cttccactgc atgacggcaa ccattatccg
     2461 ggggtcagca aaacagcgga ttacaaagcg cgggcgcaga aattctttga tgaactggac
     2521 gccttcttta ctgaacttga gaaatcgggt cgtaaagtga tggtggtcgt ggtgccggaa
     2581 cacggcggcg cgctgaaggg cgacagaatg caggtatctg gcctacgtga tatccctagc
     2641 ccgtctatca ccgacgtccc cgttggggtg aaattcttcg gcatgaaggc accgcatcag
     2701 ggggcaccga ttgtcatcga acaaccgagc agcttcctgg ctatctccga tctggtggtt
     2761 cgcgttctcg atggcaagat tttcaccgaa gacaatgttg actggaaaaa actcaccagt
     2821 gggttgccac aaacagcacc ggtctccgag aactcaaatg cagtagttat tcaataccag
     2881 gataaaccgt acgttcgcct gaacggcggc gactgggtgc cttacccgca gtaagaacga
     2941 aaaaggccgc agagtttccc ccctgcggcc tggtccgggc acaaattgcc attacggcag
     3001 cctgacgccc gcatgacacg ttacttccgc ttgttcagcc agttcacgat catactggca
     3061 agaatgccag caatgaccgg agccgctaaa tcatgccaga aggccatgcc cagctctgcg
     3121 aacgtcatat agccgcctgt gttgtaatga caacgtttcg cggctattct tgagtggtct
     3181 agagtcaaga ttagcccccg tggtgttgtc aggtgcatac ctgcaacgtg cgggggtttt
     3241 ctctctccag caaccaatgc caccagggat aaagcccccg caacattgcg cctcaccgga
     3301 acctttccgg cttgccgctg attctacgat tgctttttac tgtcagcacc cggcaatctt
     3361 cctcattgcg cgatgccttc cagcttaatt tctttatttg caatagttgc agttcatatc
     3421 cgggctgact ccagactggt agtgaaatgc gcaattttgt agaattgcgc ctcctcgcga
     3481 cctccccagt gaatctgttg cctcattttt tacatactgc gtattcgact tctccacctg
     3541 ttgcgcaaga gaaactgggt ttattcattt ttgcgaggcc gacttctttc tggacaggac
     3601 tttatgcagc acaacacact atcgaaacac aatcagaaat tgccgtttac acgctacgac
     3661 ttcggctggg ttttattatg cataggcatg gcgattggtg ccggaaccgt gctgatgcca
     3721 gtacaaattg gcttgaaggg aatttgggta tttattaccg cagcgatcat tgcttatcct
     3781 gccacctggg tagtgcagga catttattta aaaacccttt ctgaaagcga ttcctgtaat
     3841 gactacaccg atattatcag tcattacctg gggaagaact ggggaatttt cctcggggtt
     3901 atctactttt tgatgattat ccacgggatt tttatctact ctctctccgt ggttttcgac
     3961 agcgcctcgt acctgaaaac cttcggttta accgatgccg atctttcaca atctctactt
     4021 tataaagtcg ctattttcgc cgtactggtg gcgattgcgt ctggtggtga acgattactg
     4081 tttaagattt ccgggccaat ggtggtggtc aaagtaggga ttattgtcgt gttcggtttt
     4141 gcgatgatcc cgcactggaa tttcgccaat ataaccgcct tcccgcaagc ctccgtcttt
     4201 ttccgcgatg tcttgcttac cattccattt tgcttctttt ctgcagtatt tattcaggta
     4261 cttaacccaa tgaatattgc ctatcgtaaa cgggaagcgg ataaagtact ggcaacccgg
     4321 ctcgcgctgc gtacccaccg aattagttat atcacgctca tcgcggtgat cctgtttttt
     4381 gccttttcgt ttaccttctc aattagccac gaagaagccg tttctgcctt tgaacaaaat
     4441 atctcagcac tggcgctggc cgcgcaggtg atccctgggc atatcattca tatcacctct
     4501 acggtgctta atatctttgc cgtactgacc gcattctttg gcatttatct cggtttccac
     4561 gaggccatta aaggcattat tctcaatctg ttaagccgaa ttattgatac caagaaaatt
     4621 aactcacgcg tgctgactct ggcgatctgc gcttttatcg tcattacgtt gacgatttgg
     4681 gtttcgtttc gtgtatcggt gctggtgttc tttcagttgg gaagcccgtt atatggtatt
     4741 gtgtcgtgcc tcattccgtt tttcctgatc tataaagtcg cacaactgga aaaacttcgc
     4801 ggatttaaag cctggctgat tctgctgtac ggcattttgc tatgcttgtc gccactgttg
     4861 aagctgattg agtaaaccgg agcgcatggc cccggttttg tgagttaacg ctgcggattt
     4921 tcatcctgat caacagcaaa acaagctacc agttgaccgc cgtagtcttt tagctgcggc
     4981 tgcaactggg tgcaggggcc gaagcgccga cgacagcggg cgttgaaggc gcaacccggc
     5041 ggtggattca gtgggcttgg tagttcaccg ctgagcttga tgcgctcgcg gcgatcgtcc
     5101 gggttcaggc gcggcgtcgc ggaaagtagc gcctgagtgt acggatggcg cgggttattg
     5161 aagatttggt ctttcgttcc cttctccacg cagcggccca ggtacatcac catcacttca
     5221 tcagcaatgt gctccaccac cgacaggtcg tgggagataa agacataaga cagccccaac
     5281 tcctgctgca aatccatcat cagattcagc acctgcgcgc gcactgaaac atccagcgcg
     5341 gaaaccggtt catcggcaat caccacatcc gggtcgagca tcagaccacg ggcgatggcg
     5401 atacgctgac gctgaccgcc ggagaacata tgcggatagc ggtcatagtg ctcggttttc
     5461 aggccgactt tcgccatcat cgacagggct ttttcccgac gctgttcttt gcttaagctg
     5521 gtgttgatca gcagcggctc ttcaagaatt tgcccgactt ttttacgcgg attcagcgaa
     5581 ccgtaagggt tctggaagac gatctggatt ttctgccgac gcagcttctg cgcctgcgga
     5641 tcgtgcttaa gcagatcctg cccctggtaa tacagctcgc caccggtggg catttcaatc
     5701 atcgtcagca accgaccgag ggtcgattta ccgcagccag attcgcccac tactgccagc
     5761 gttttgccac gttcaaggtt aaacgaaacg ccatccagcg ctttaaccag acgttccggc
     5821 gcgaacatgc ctttcttcac cggataatgt tttttcaggt cgatagcctg caacagcggt
     5881 tgttgcaggg tggcctcttg cgtactcata gtgtcggcct cccggcatca tcaagtgggt
     5941 aatggcattt ggactgacgc ccgtcagcga gcatattcag cgccggttct tcagcgcgac
     6001 atctgtcagt ggcatagggg cagcgcgggt taagcaggca gccgttcggg cggtcgtact
     6061 tgccgggaac gacacctggc aacgacgcca gacgttcttt gtcctgagca aattctggca
     6121 gcgcacgcag caatgcctga gtatacgggt gacgcggcgc atggaagatg gcgtgcgcat
     6181 caccggtttc caccacctgg cctgcataca tcacgatgat tttatgtgcc gcttccgcca
     6241 ccagcgccag gtcatgggta attaacacca gcgccatgtt ctctttctgc tgtagctcca
     6301 gcagtagttc gatgatttgc gcctgaatgg tcacgtccag cgcggtggtc ggttcatcgg
     6361 caatcagcag ttttggccga caggcaatcg ccatggcgat catcacgcgc tggctcatgc
     6421 cgccggaaag ctgatgcggg taaacatcca gacgcgatgc cggatcggga ataccgacct
     6481 gattcagcag gtcaatcgct cgctgacggc gggtactttt gttgccgccc tgatgcacct
     6541 taatcgcttc cataatctgg aaacccacgg tgtagcacgg gttaaggctg gtcatcgggt
     6601 cctggaagat catcgccact tcggcaccca ccaggttgcg gcgctctttt tctgagatac
     6661 gctgcaaatc ctggccgtta aactccagtt tttctgccat tacgcggccc ggataatcaa
     6721 tcagccccat aatcgccagt gaactgaccg acttaccgga gccggactca cccacaatcc
     6781 cgaccacttc gccctgtttt acgctgtagc tgatgcggtc tacggcgcgg aacggcgcgc
     6841 tttcgtcgcc gaaatgcacc gataatttat ctacatttaa taacgccatc tcgaacctct
     6901 tactgcttca gtttgggatc gagcgcgtca cgcagaccgt cacccatcag gttaaatgcc
     6961 agcaccgtca gcaggatcgc cagacccggg aaggtcacga cccaccaggc gctttgcgcg
     7021 aactgcaaca cgtcggagag catggtgccc cactcaggcg ttggcggctg tgcccccatg
     7081 ccgaggaaac caagagcagc catatcgaga atggcgttag agaaaccgag cgacgcctga
     7141 acaatcagcg gcgcaaggca gttcgggaag atgttaataa acatctgacg catcgccccg
     7201 gcacccgcca cgcgagacgc ggtgacgtaa tcgcggttaa cttccaccag cacggcggcg
     7261 cgggttaagc gcacatagtg cggcaaggca acgaaggtca gtgccagcgc ggcgttacca
     7321 atcgacgggc cgaaaattgc caccagcacc agcgccagca gcagacttgg cagcgccagc
     7381 atgatatcga ccacgcgcat aatgatgtta tcgaccaggc cgccaaagta accggcgatc
     7441 aggccgagaa taacgcccat aatcagcgat aacacaacta ccagacagcc aaccagcagc
     7501 gacaggcgcg caccgtacat caggcgcgac agcacatcac ggcctacgtc atcggtgccc
     7561 agcaagtgcg ccatgctgcc gccttcctgc caggctggcg gggcgagcag tgcatcgcgg
     7621 aactgttccg ccgggttata gggtgcaatc cagttggcaa agatcgcgat gaacagcacg
     7681 atgacgacgt aaaccagccc gacgaccgcg cctttgttgc gtttaaaata gtgccagaac
     7741 tcctgtaacg gggtcatcgg caccggtgcg ctaatcactt tattttcagt aacctgtgac
     7801 atgatgtccc cttacttctt atgacgaata cgcgggttca ccacgccgta cagcagatcg
     7861 accagcaagt tgacgaggat aatcatcgtc gccaccagca atacgccgcc ctgcactacc
     7921 ggatagtcgc ggcgttgcag tgcgtcaatc aaccagcgtc ccagaccggg ccacgagaag
     7981 atggtttcgg tcagaatcgc ccccgccagc aatgttccca cctgcaggcc gataacggtc
     8041 accaccggca gcatcgcgtt acgcagcgca tggacgataa tcacccgcat gcgggttagc
     8101 cctttggcgc gcgcggtgcg gatgtaatcc tcgcccagca cttccagcat cgaggagcgt
     8161 gtcatacgca caatgaccgc cagcggaata gtacccagca caatggcagg caagatcata
     8221 tgggcgacgg catcgataaa gttgccgtct tcaccccaga tggcggtgtc gattagcata
     8281 aaaccggtta acggattgga gtcatcgagg aacaccatat cgctcacgcg accggagacg
     8341 ggcgtcaggt tccagtgcac cgaaaccagc atgatcagca tcatgcccca ccagaagata
     8401 ggcattgaat aacctgtcag cgccaggcca accgctgtgt gatcgaaaat ggaaccgcgt
     8461 ttaaccgcag ccagcacgcc gaccggaata ccgactgccg tagcaaaaat catcgcgcag
     8521 acgccaagtt ccagcgtggc ctggaagcgc ggcacgaact cttcccaaac cgggatgcgg
     8581 cttttcattg aaatgcctag atcgccatgc ataacgcccc aaatgtaatg gagatactgc
     8641 tgccacatcg gtttatctaa gccgagttca gccagcagct gcgcgtgacg ctctggggag
     8701 atcccacgtt cgcccgccat gatcatcacc ggatcgcccg ggatcatgtg gacaaaggca
     8761 aatgtgagaa gggtaatacc gataaacgtg gggatgacga gtcccaaacg tcggagaata
     8821 aactgcaaca taacccggat tctctatagt gacccgcagc ctggaactgc gtgtctgtat
     8881 tgctcacaaa tcaattcccg cccttatcgg acagggaatg aagtattgct gccggatggc
     8941 gctgcacgca gaggcacagc gccaccgggt atacagattg ttgacaaagt gcgctttgtt
     9001 tatgccggat gcggcgttaa cgccttatcc ggcctacaaa atcgtgcaaa ttcaatacat
     9061 tgcagaattt tcgtaggcct gataagcgga gcgcatcagg catttttgcc tttgccatca
     9121 gtcttgtatg gcttttaatt attcgataga gacgttttcg aagtgatgtt tgcctaatgg
     9181 atcaaccaca tagcctttaa cttctttacg taccggttca aacacggtgg agtgagcgat
     9241 gatcagtgcc ggagcctgat cgtgcatcac cacctgcgct tgtttgtaca gttcaacgcg
     9301 tttattgtgg tcgtcggtag cacgcgccgg ttgaatcaga tcttcaaacg gtttgtagca
     9361 ccattttgag tagttggagc cttgttcaga ggcggcgcag ctgaacaggg tggcgaagaa
     9421 gttatccgga tccccgttat cgccagtcca gcccatcatt accgtctggt gctcgccatc
     9481 tttcgcacgc ttgaggtact caccccattc gtaggtgaca attttggcct gcacgccgac
     9541 tttcgcccag tctgcctgaa tcatctccgc catgcggcga gcgttcgggt tatacggacg
     9601 ttgtaccggc atcgcccaca ggtcgatgga gaaacctttt tccagacccg cttctttcag
     9661 caaggctttc gctttttcag gatcgtaggt gtagtcctga acgtcgtcgt tatagcccca
     9721 catggttggc gggatcaggt ttttcgctga tacgcccgcg ccctgataaa ccgctttgat
     9781 gatcgcgtct ttgttcaccg cgtaggtcag agcctggcga actttcacgt catcgagtgg
     9841 ttttttctgc acgttatacg agagataacc gacgttcagc cccggcattt ccatcagatt
     9901 gatggattta tcctgcttca tgcgagcgat atctgccggg ttcgggtacg gcatcacctg
     9961 gcattcattc ttctgcaatt tcgcgtaacg cacggaagcg tcaggggtaa tagagaaaac
    10021 cagcgtatcg atctgcggtt tggtgcccca gtagccatca aacgctttgt agcggatacg
    10081 ggaatctttt tgatactgct gtaactggaa cggaccggtt ccgattgggt tgaggtccag
    10141 tttttccggt gtaccggctt tcatcatcgc atcagcatat tcttttgaca gaatagaggc
    10201 gaagtccatt gccaggtcag cgaggaacgg cgcttccggg cgagtcagca caaactgaac
    10261 ggtgttgtcg tccacctttt tcacttcact gatcagctct ggcaagccca tgccttcgaa
    10321 gtattcgtag ctgccgccag aaactttatg gtacgggttt tgcgcgtttt tctgacgatc
    10381 gaacgagaac accacatcat cggcgttcag ttcacgcgtc ggtttgaatt ctttattgtc
    10441 gtgccacttc acacctttac gcagatggaa ggtataggtt ttaccgtctt cgctgacttc
    10501 ccacttttca gcgaggcccg ggatcacttc ggtggtgccg attttaaatt caaccagacg
    10561 gttataaagc gggacggaag aggcgtcata ggtggtgccg gaggtaaaca gctgcgggtt
    10621 aaacccttcc ggagatcctt ctgagcaata aaccagagtt ttagcctgaa cacttgctgc
    10681 gacggtcata gccaccaggc tgagaccaag cttcagcatc cctgactttt tcaaggaaat
    10741 acgcattatt ctgctccaat tgtgatgttt gttgttttaa ccctttgcag tgggttgtcg
    10801 ctgcctgacc ttttttgttt tttgcccggt cgggtcaacg ttatgaggtg gggatgccgt
    10861 actaattaac atcagtgtaa cagtgcggat cctccataaa atgcccctcg tgacctacaa
    10921 tctgtcaaca gaatgtgaaa acgtcaatac agccaaccgg gatttacacc aacggtgaga
    10981 atccacaaac aaagattaaa aaaacttcaa acagctattt gcagcagaga aatttgtgct
    11041 actccaacat gaccagaaca atcagcttaa tatttagcaa catttggtga ttaagtttta
    11101 tgcatttaat gaaaaaaatc tgaggaaaag gtggatatct gagcgattaa taccatgaac
    11161 gctaaaacgc acagcggaaa atgccagggc cagccataag taacgcaaaa aaagatttaa
    11221 gcaaatataa aaaaagacaa tggtttctgt cacaaatccg ttaatggata gtgagatatg
    11281 gggcgcaaat ttggcaggga aatgtgtgtc gtgtaggtcg aataaggcgg tcgcgccgca
    11341 tccgacaatg gccggcacat ttgcctgatg cgacgctgac gcgtcttatc aggcctacgt
    11401 cgagtcgaac ctccctgccc ccggaggttc tcatcctcat gagctgcaga tgcaaaaaag
    11461 cctgctcgtt gagcaggctt ttcgaatttg gtcggtgata gaggattcga acctccgacc
    11521 ccttcgtccc gaacgaagtg cgctaccagg ctgcgccaat caccgaatgc ggggcgcatc
    11581 ttactgcgca gatacgccct cgtcaatccc ttaatagcaa aatgcctttt gatcggcgag
    11641 aaagtcagca ggccgcttag ttagccgctg cctcttttgc ctgcgggatg tgacaccagt
    11701 tgttgttttc gttaattcca ccatccggtg aagtatagcc aagacaaccc atgatggtat
    11761 cgtacagctc tacgtgacgg cgtggcactt tcatgtcggc ttcttttttc agctgcgcaa
    11821 acgcctgcgc attggccgga ttttccagat atttatctga catccagacc atcatcggta
    11881 cgcggaactg ctccggcggt gccagttcac gcggcgtgcc gtgcaggtgc tcgcgttcat
    11941 taattgactc accgtggtca gctgcgtaga acacaatcgc tttcttatcg cgaacctgat
    12001 caatcacgct ggagataaag tgatccacat aggtcaccga gttgtcatag gagttgatca
    12061 tctgcgcttt ggtacagccg ctatcaacac caatacattc cggcttccac tgcgcgaagc
    12121 tacgcggata acgctgggtg tagttaaaat gcgaaccttt ggtatgcaga atgatcagat
    12181 gcttaccatc cgggttgcgc cctagcgatt gctgcatttc gtctaccagc aacatatcat
    12241 ctaccggctt gccacgatta cgtggctccg caccaatctg ctcacgataa gcaatgttgt
    12301 ccgccatcgt gttgctgtag aaccacattt cgctctgcat agcgtagagg tcagaactga
    12361 atcctaactg cttcagaacc gcgaaaatgt tctgttcttt taatgtgcgc tgcggattat
    12421 cttccgcgcc cccctgacgt acaaacatgc aacgcagtga gagtttggtt gcggtatcac
    12481 aggagtaacc acggaacgcc gccagatttt tctcctgggc cagtttcggc gtggtattac
    12541 gctcatagcc gaaaataccc atatggtccc aacgcgtggt ttcaccgatg ataaacacga
    12601 cataagtgtc atcaacgttt tgcggtgcct gataagtgaa tttcttcgcc ggattaagca
    12661 atgaattatt atcggaagat tcatcgaccc gcgcccaggc atacagcccc aacgcagaaa
    12721 gccagtttga tggcagataa gagttcgcta cgacaccgcc ataactcggc aaatcaacgc
    12781 cggtcgccct ctccactttc ttctgctgga tatccagcaa acgaatcggt gcccaaacca
    12841 taatacccgc cagtacgacg accgccaggc tgcgaatacg ctgccctggg gttcgcagtt
    12901 gtcggagcaa ggtgtagcga cagcggttat tccagataag gataagaggc aatgcactaa
    12961 cggcgattaa ccagagaata aagttcagac caacaacttc ttttgacagg tcgatatcgg
    13021 tggtcatgac ggaagcgatg atgccataac caatgaccac attaaggaag gtcatgtaat
    13081 agctggcacc tgcggaaaag agcaccacca gcgatgccag aatacgccag ctgcggcggc
    13141 caaacagcga aagaagacgt agtaaaaaga aggtcaccag tacggtggcg gccagttcaa
    13201 caacagcaga aatgcctttc cagacggtaa aatcgtgcgc atagctgccg aagcggcggt
    13261 aaaaaaccgc gccattcata aaaaggccaa tatagattgc aagcaaaaag ctcagcttct
    13321 gctgtgtaat cgatttgatg tatctcatgc aaacaaaccc tggacaaaca ggtgataaca
    13381 ccctgcttag tggcttttca ggcccggcag ggaaaaagtg cggaggtagt cagataagta
    13441 aaatctacac gcgcgaataa gtagaccaca gcaaagggaa aaagtgtggg gaaagagtgt
    13501 gcatgaagca caaatttaca aaaatgcaag tttgaaaaga gactttagcg ttacaaaaag
    13561 cagtaaaaag ggccagttaa ttggcccaaa ttttacgagt cattgtttat acatttcgtt
    13621 gatgcgtttg atgcacacgg aagctgaagc ccagtagctc gcggctgagc attaaaggga
    13681 gccatgcgcc tcacgcaaca ttttctgctc tggctgacga atcgtcgtag aaagcgccaa
    13741 tgacagaatc agcagggcga aaatcacgta gaaggtcaca tagaagccgc caaacagtga
    13801 ggcgataatc gaaccacaaa tgctaccgat accgaaaccg agataaatca caccgtagtt
    13861 tttcgccagg ttattgaggc caaagaactc actgaccagt gacggaaaga cggtaatagt
    13921 gccgccaaag ttaaatgcca cgcaggcaat cgctgcaaag aacgtcactg cattcaatgg
    13981 tgcaaacagc agggccgcca tacccaccag cgatatcacc tgaccaatgg taataacacg
    14041 gatacgggcg attttgtcag acagaatacc cagcaccaga cgacctgaaa ggttggcgat
    14101 ggaaataaca gtgactgcat tggctgcgga aaccacatca aggtgtgcca gactttgggc
    14161 gatatctttc gctaccccaa tcacgtacag gccgctcatg caggcggtca ggaacattac
    14221 cgctaacatc cagtactgcg gtttacgcat cgactctgcc agcgtgtaat ctttctccac
    14281 cacaccattg ctggttttca cttcctgttt tggtgcgtct ttcattaacg ttgcgccgaa
    14341 aacaatcatc aacagcgcaa tcgctcccca aatcacaaag gttttttcca gaccgaccgt
    14401 ttccagcagc tgcgtgtcga taaatttgaa acccaggcta cccagaccat aagaaccgat
    14461 agcgaacgcg gagatcagac ctttacgctc cgggaaccac ttcacacagt tagagagcgt
    14521 cagcagataa cccgcgccat ctgccagtcc caccagcaca ccggcgctta accacagcat
    14581 catcaggttg tcagaatgcg ctgtcaggaa gaagcctaat cccagcaaaa tgccggaagc
    14641 catggtgacg cgtttaacgc caaaacgttc ctgtaatttg cccgcaacag aagacgaaat
    14701 tgccagcccc agacttaaca agccgaaaga gaaagcgacc tggcttaccg gcgcatccag
    14761 cttggcggaa agcgcgccat taaacaggct ccaggtataa accgacccca gcgcaaactg
    14821 ggtaatgata gtaccgatga gtgtcagcca gcgggtacgc tgataatttg aaggtgtcat
    14881 ggcagtattc ctgcagtaat aaaaaggaaa aatctctgcc gacaaccata acgaagtgca
    14941 ctttatgcct ggggaaaaac ggcatgaaat gccatcagaa cggaatgaaa tgccttagtt
    15001 caggaatgaa tgacgcgaca attattcacc aaatttatta tttggtaagt tagcacccac
    15061 tatcgccaca actattgtta tttaatggtt tcaattgtga ctgttacgtt aaaatctggc
    15121 gctaaccccc atcgtataca gatagtcgct attattagtg gtattttctg cctgagttag
    15181 cgcggcatag gcggcaatat tttgattgag caacatatca gcgcctacgg tgacatccag
    15241 ccagttgccg ttctggtttg tcgccgtcat ccggctcagg cctgattgcg ccttccagat
    15301 attctcgcca aattgctggt tatagctgat ttgcgcccag ggtcggagat cgccaagccg
    15361 ggagtcaaca cgccagccta aggtactcac gctggcatgc cataacggat cggtcagcgt
    15421 ggtagtactg tcgccaaact cgttatacat cgaggtggtt gtgccatcgt aatgtaatgc
    15481 cgcgaccggg ccggttgtaa cctgttcata taaaggaaaa ttccagccca tactcatact
    15541 gctggtcaca tccacagggg tttcttccgc cagattgact tccagtccca gcgccctttg
    15601 gctactttga atacgttgcg acaaaatatc gttgtaatca ggttgatgat cactatccca
    15661 ggtgtaacgc tctgtggaaa gtccgggttg cgtatcaacg atatattctt gttgccaggc
    15721 gtaagctgtg ttgagaaaaa aggcactgat taccacgctc gccagcaggc ttagctgcca
    15781 gcgaccaccg ctttttttta tgatcattaa cacgactcca gggaagagcc gaattcggca
    15841 atattattgt cattgtatga aggatatcgg gcatagtagc cctgtattaa atattgactt
    15901 tttcaccgat gcgtcaagaa aagcggctga aatttttacg atcgggtaca tagcgaggga
    15961 aagtatggaa cgttgcggct gggtgagtca ggacccgctt tatattgcct accatgataa
    16021 tgagtggggc gtgcctgaaa ctgacagtaa aaaactgttc gaaatgatct gccttgaagg
    16081 gcagcaggct ggattatcgt ggatcaccgt cctcaaaaaa cgcgaaaact atcgcgcctg
    16141 ctttcatcag ttcgatccgg tgaaggtcgc agcaatgcag gaagaggatg tcgaaagact
    16201 ggtacaggac gccgggatta tccgccatcg agggaaaatt caggcaatta ttggtaatgc
    16261 gcgggcgtac ctgcaaatgg aacagaacgg cgaaccgttt gtcgactttg tctggtcgtt
    16321 tgtaaatcat cagccacagg tgacacaagc cacaacgttg agcgaaattc ccacatctac
    16381 gtccgcctcc gacgccctat ctaaggaact gaaaaaacgt ggttttaagt ttgtcggcac
    16441 cacaatctgt tactccttta tgcaggcatg tgggctggtg aatgatcatg tggttggctg
    16501 ctgttgctat ccgggaaata aaccatgatt cgggaagcgc aacgttcaga actccccgcg
    16561 atcctcgaac tgtggctgga aagtacaacc tgggggcatc cctttataaa agcgaattac
    16621 tggcgtgact gcattccgct ggtgcgggat gcctatcttg ccaacgcgca aaactgggtc
    16681 tgggaagaag acggtaagct tctcggtttt gtcagcatta tggaaggccg atttctggca
    16741 gcgatgtttg tcgcaccgaa ggccgtcagg cgcggtattg gtaaggcgct gatgcagtat
    16801 gtgcagcagc gccatcccca cctgatgctg gaggtttatc aaaaaaatca accggcgata
    16861 aatttttacc aggcacaggg ttttcacatt gtcgattgcg catggcagga tgaaacccaa
    16921 ctacccacat ggattatgag ctggccggtg gttcaaacgc tgtaagtgtc agttccggac
    16981 cgttgtattt ttccagccat gccagcgccg tattacccgc acagccattc cccagccgcg
    17041 agctggggag atctttggtc agcacgttca ctgcgccgtt tttacaaata ccgtcagcgg
    17101 ttaaatccag atccggccat gccccttcgt gaatgcaaat cacgccaggt ttaattccct
    17161 cgctaatgac cgctccggca agaatttgcc cgcgtgcgtt ccacaaccga acagtatcgc
    17221 catcttgtat gccgcgctcc tgggcatcgt caggatgaat ggtgacaggc tcacgatttg
    17281 ccaccgcgta caattcgcgc agagaactgt aattcagctg gctgtgcagg cggtgcgccg
    17341 gatgggcaga aagtacctgc aactgttctg gttcggcatt gccctgccat tcgtccggct
    17401 ccagccacat tggatgccca gggcaatccg ggtaaccgta atcggcaata cgctgtgaga
    17461 agatttcaat cttgccgctg gcggttttta acggatgcgc cagcggatcg cggcaaaaat
    17521 cagcgaagcg aataaaccgc tcgctgtccg ggttttccgg catctcaatt aactggttgg
    17581 cttgccagaa ctcagcaaat ggcggcaatt caacctgctg gcttgccccg cgctgtcggg
    17641 caacgttata aaacgtttcc agccattgca gctcactttt tccttccgta aaacgtgcat
    17701 aaccgccctt ctcccagcgt tcacttaact cggcaaaaac atcaaaatca ttacgcgctt
    17761 catagcgtgg cggcaccact tgcttcatcg gcaccagatg ctgattactg taatcaccgg
    17821 tcatggtgag atcattacgc tcaaaagagg tagtcgcagg cagaacgata tccgcgtgtt
    17881 ttgccgccgc cgtccagaag cattcagaga tcaccaccag ctccggtttt tgccaggcac
    17941 ggatcaggcg attggtatcc tgatgatgag taaagttggc accgcccgcc caccagataa
    18001 aacgaatatc cgggaaatgt cggttcatac cgttgtgttg atatgcgcca ccagggtttt
    18061 ccagtgcttc aacaatgcgg gcaacaggga ttttatccac cgcatcgcag ccacccggca
    18121 agctgccctg catggaagag agcaccgcag aacgccgcgt ggggttacca ccattggcaa
    18181 aatggtaaga aagaccaaaa ccgccgccgg gtgtgccgat ttgccccaac attgctgcca
    18241 gcgtgacgat catccagtgt ttttgctcac caaactgttg gcgctgcatt ccccagcctg
    18301 ccatcagcat ggtggtattt tggtggaaaa tagccgccag ctcgcggatt ttcgctgcgc
    18361 caacaccaca aatctctgct gcccattcgg cggttttcgc tattccgtca ctctcgccca
    18421 gcaaataaga ggcgaagacg gcataacctg tggtgcaacg cgccagaaac gcttcgtcgt
    18481 gacaaccatt ttccaccagc gtatgggcga tccccagcat cagcgcaaca tcggtgccca
    18541 tgtgcggtgc cacccactcc attttatcgc caaagaaatc gacggtttcc gatcgcattg
    18601 gatcaatgca gatcagcttt ttcccgctgt cacgcagtgc agaaaagtaa gaaagcccct
    18661 gctcatcgga tgcattccac gcaattttca gcgtattgag tgggttagca ctccacagca
    18721 ccacgacatc gctatgttcc agcaccagcg gccaactggt ctgctgttga taaacttcac
    18781 taccacccac gacatacggc atgatcgcct gtgccgcgcc ggtcgaataa tcccccagat
    18841 gcccggtata accgcctgcc agcgccatat agcgttgtaa taatgtcgag gccttatgca
    18901 gcacgccgtt tgaacgccag ccgtaggaac cagcaaaaat cgatgccgga ccataagcct
    18961 cacgaatgcg tttatgttgt tggtgaataa gatccagcgc ctcatcccaa ctcacgcgaa
    19021 caaattcatc ctgcccacga atgccttgcg ggttttccgg tgacgcaaga aagccttttc
    19081 gcaccattgg aaatcgtacc cgcgtattgc tgtgaacctg gtcgcgaacc gcgctctgca
    19141 aggagttttc cattcctgtg gctaacgcgc cacgcgagct aaacacggtt tcgccgtcgg
    19201 tttcaaccag catgggcccc caatgggcgg cagtcagaac ggaatatcgt gaggatgagt
    19261 tggccaaacc gaagactcct gaaaggttag atgtattgag cagttaaata ttacttacaa
    19321 atttccgagt attcccagga ataatcttca tgttcacgcg gcataatctc ccgccacgga
    19381 acccgtggca aagaataaaa aggttattaa ggattaacaa tgaagaaacg tgtttatctt
    19441 attgccgccg tagtgagtgg cgctctggcg gtatctggct gcacaactaa cccttacacc
    19501 ggcgaacgcg aagcaggtaa atctgctatc ggcgcaggtc tgggctctct cgtgggcgcg
    19561 ggtattggtg cgctctcttc ttcgaagaaa gatcgcggta aaggcgcgct gattggcgca
    19621 gcagcaggcg cagctctggg cggcggcgtt ggttattaca tggatgtgca ggaagcgaag
    19681 ctgcgcgaca aaatgcgcgg cactggtgtt agcgtaaccc gcagcgggga taacattatc
    19741 ctcaatatgc cgaacaatgt gaccttcgac agcagcagcg cgaccctgaa accggcgggc
    19801 gctaacaccc tgaccagcgt ggcaatggta ctgaaagagt atccgaaaac ggcggttaac
    19861 gtgattggtt ataccgacag cacgggtggt cacgacctga acatgcgtct ctcccagcaa
    19921 cgtgcggatt ccgttgccag cgcgttgatc acccagggcg tggacgccag ccgcatccgt
    19981 actcagggcc ttggcccggc taacccaatc gccagcaaca gcaccgcaga aggtaaggcg
    20041 caaaaccgcc gtgtagaaat taccttaagc ccgctgtaat ccctttcatg ccaggtgatg
    20101 cagaaatcac ctggcattct tcagttttcc ttcatcatat ttcaggctaa ggtgatcgcc
    20161 ttatcagtga atggagagaa gcatgaagcc gtccgttatc ctctacaaag ccttacctga
    20221 tgatttactg caacgcctgc aagagcattt caccgttcac caggtggcaa acctcagccc
    20281 acaaaccgtc gaacaaaatg cagcaatttt tgccgaagct gaaggtttac tgggttcaaa
    20341 cgagaatgta aatgccgcat tgctggaaaa aatgccgaaa ctgcgtgcca catcaacgat
    20401 ctccgtcggc tatgacaatt ttgatgtcga tgcgcttacc gcccgaaaaa ttctgctgat
    20461 gcacacgcca accgtattaa cagaaaccgt cgccgatacg ctgatggcgc tggtgttgtc
    20521 taccgctcgt cgggttgtgg aggtagcaga acgggtaaaa gcaggcgaat ggaccgcgag
    20581 cataggcccg gactggtacg gcactgacgt tcaccataaa acactgggca ttgtcgggat
    20641 gggacggatc ggcatggcgc tggcacaacg tgcgcacttt ggcttcaaca tgcccatcct
    20701 ctataacgcg cgccgccacc ataaagaagc agaagaacgc ttcaacgccc gctactgcga
    20761 tttggatact ctgttacaag agtcagattt cgtttgcctg atcctgccgt taactgatga
    20821 gacgcatcat ctgtttggcg cagaacaatt cgccaaaatg aaatcctccg ccattttcat
    20881 taatgccgga cgtggcccgg tggttgacga aaatgcactg atcgcagcat tgcagaaagg
    20941 cgaaattcac gctgccgggc tggatgtctt cgaacaagag ccactgtccg tagattcgcc
    21001 gttgctctca atggccaacg tcgtcgcagt accgcatatt ggatctgcca cccatgagac
    21061 gcgttatggc atggccgcct gtgccgtgga taatttgatt gatgcgttac aaggaaaggt
    21121 tgagaagaac tgtgtgaatc cgcacgtcgc ggactaagcc gcgactgcgt agagtaaagc
    21181 ccgataatcg ctcgggcttt tactctttat tgggttgcag taactgctgt agtccaggcc
    21241 tgattaaacg cctgatgctg tgccggtaat ggcgcaatca gtttgttata ttcacttgcc
    21301 tgctgtgaag tcgggaactg gatgccattt gcgacaaagc tcacctgagt accttgctga
    21361 gcaatgtagt ctccaaccat caccagttgt tgcgtgaaag tttgtgccgc cggaatcaac
    21421 ggttgcaatg catcagctgg cgtcgttacc actttggtga atgcctgatc aaagaccggt
    21481 ttcaaatcat cgctctgttt tagcgcagag tgcgcagcat cggcctgcaa cttcgcattt
    21541 tgcagttgtt gcgccagcac acccagcgaa ccgttcattt cacgcagcgg accgctttgc
    21601 gtaacgtaat cctgcggtac gcgaattgcg ttaacgctgt ctacaaccgg gcgcagaccg
    21661 gaatccatcg cctgattcac ctgctgagaa taaccataca gaatcgcgta atcagagaca
    21721 aaaggaccaa actgtttttt ctgatcggca gtcagggttg gtagacgttc accgctacgc
    21781 atcactgtat tctgcaggaa gtcgataaac gctttacgct gatcgccttc tttatcaaaa
    21841 cacccactca ggctaactac cattaataac gccgcaagag gcgcaaacca gcgagagcag
    21901 gactttcctg tcgccatttt cttactcctt tcacccaaaa aaagcgcaca acgacacacg
    21961 cgtgtctgac gtgacaagga tagtccagga caggcttgca ggataccctt tctacgtaat
    22021 cttcctggta aaatcatgga gaaaatgaaa caaggttaat ttgttgtttc attgttaaaa
    22081 aatgatagtg ccgttgcctt ttttgtagcc agagatttac gccttcgcag aaggttagat
    22141 tttcgaaatc tttacaaaac aggagtggta agagatatat cggcatttat cagatcgtta
    22201 ggtggctata agtcacgcta aatgacaggc tgaatcgaat catagccaga gcatgccctg
    22261 acttcacccc gctgtgtctg cttttcccga ctattcttaa tgagcttcga tgcaattcac
    22321 gatcccgcag tgtgatttga ggagttttca atggaatata aagatccaat gcatgagctg
    22381 ttgagcagcc tggaacagat tgtttttaaa gatgaaacgc agaaaattac cctgacgcac
    22441 agaacaacgt cctgtaccga aattgagcag ttacgaaaag ggacaggatt aaaaatcgat
    22501 gatttcgccc gggttttggg cgtatcagtc gccatggtaa aggaatggga atccagacgc
    22561 gtgaagcctt caagtgccga actaaaattg atgcgtttga ttcaagccaa cccggcatta
    22621 agtaagcagt tgatggaata gacttttatc cactttattg ctgtttacgg tcctgatgac
    22681 aggaccgttt tccaaccgat taatcataaa tatgaaaaat aattgttgca tcacccgcca
    22741 atgcgtggct taatgcacat caacggtttg acgtacagac cattaaagca gtgtagtaag
    22801 gcaagtccct tcaagagtta tcgttgatac ccctcgtagt gcacattcct ttaacgcttc
    22861 aaaatctgta aagcacgcca tatcgccgaa aggcacactt aattattaaa ggtaatacac
    22921 tatgtccggt aaaatgactg gtatcgtaaa atggttcaac gctgacaaag gcttcggctt
    22981 catcactcct gacgatggct ctaaagatgt gttcgtacac ttctctgcta tccagaacga
    23041 tggttacaaa tctctggacg aaggtcagaa agtgtccttc accatcgaaa gcggcgctaa
    23101 aggcccggca gctggtaacg taaccagcct gtaatctctg cttaaaagca cagaatctaa
    23161 gatccctgcc atttggcggg gattttttta tttgttttca ggaaataaat aatcgatcgc
    23221 gtaataaaat ctattattat ttttgtgaag aataaatttg ggtgcaatga gaatgcgcaa
    23281 cgccgtaagt aaggcgggaa taatttcccg ccgaagactc ttactctttc aatttgcagg
    23341 ctaaaaacgc cgccagctca taactctcct gtttaatatg caattcacac agtgaatctc
    23401 ttatcatcca ggtgaaaaat aaaagcgtga aacaaatcac tattaaagaa agtaatctat
    23461 atttctgcgg cattcccagc ctcctgtgtt gatttccaac gagtatgtac tgcacccatt
    23521 ttgttggacg atgaaatgga atagccccta atatgtcaaa gccaaaatac ccttttgaaa
    23581 agcgccttga agtcgtgaat cactacttca caactgatga tggttacagg atcatctcgg
    23641 cacgttttgg tgtcccccga acccaggtca ggacatgggt tgccctctat gaaaaacatg
    23701 gagaaaaagg tttaattccc aaacctaaag gcgttagtgc tgatccagag ttgcgtatta
    23761 aggtcgtgaa agctgtgatc gagcagcaca tgtcccttaa tcaggctgct gctcacttta
    23821 tgcttgctgg tagtggttct gtagccaggt ggctgaaggt ctatgaagag cgcggagaag
    23881 ctggtttacg cgcgctcaag attggcacca aaagaaacat tgcaatatca gttgatccag
    23941 aaaaagcggc atcagcattg gagctgtcaa aagaccgacg cattgaggat cttgaaaggc
    24001 aagttcgatt tcttgaaacg cggcttatgt atctaaaaaa gctgaaagcc ttagctcatc
    24061 ccacgaaaaa gtgaaagtac tcaacgagct aaggcagttt tatcctcttg atgagcttct
    24121 cagggctgcg gagataccgc gcagtacgtt ttattatcat ctaaaggctc tcagcaagcc
    24181 tgacaagtat gcggacgtta aaaagcgtat tagtgagatt tatcacgaga atagaggccg
    24241 atacggatac cgtagggtaa cgctgtctct tcatcgagaa gggaaacaga ttaaccataa
    24301 agctgttcag cgcctgatgg gaaccctctc acttaaagca gcgattaagg tcaagcgata
    24361 ccgctcttac agaggagagg tagggcaaac cgcccctaat gttctccaaa gagatttcaa
    24421 ggctacgcgg ccaaacgaga agtgggttac cgatgttact gaatttgcag tcaatgggcg
    24481 caagctgtat ttgtctccag taatagatct cttcaacaac gaagttattt cttacagcct
    24541 ttcggaaaga ccagtgatga acatggttga gaatatgctc gatcaggcat tcaaaaagct
    24601 taatcctcac gagcatcctg ttctgcactc tgaccaggga tggcagtatc gtatgagaag
    24661 atatcaaaat atccttaaag aacatggtat taaacaaagc atgtccagaa aaggcaattg
    24721 tctggataat gctgtggtgg agtgtttctt tggaacctta aagtcggagt gtttttatct
    24781 tgatgagttc agtaatataa gcgaactgaa ggatgctgtt acggaatata ttgaatacta
    24841 caacagcaga agaattagcc tgaaattaaa aggtctgact ccaattgaat atcggaatca
    24901 gacctatatg cctcgtgttt aactgtccaa ctttttgggg tcagtacaaa ctttgattta
    24961 tagtcaggtg gggcttttct ctgtctgcct ttcggtgaat acctgagaca aacagtctca
    25021 agcacccgtg gctattctag cttaataagt ttgtttcttc tccttgatat aatcctaaaa
    25081 aaatctcata aaattaatat atgagataat ctttattcag cagaagatta ttaaaggttg
    25141 ctgtattatt tagcgataaa aaaagcctgc cagatggcag gctatttaat aacggcgtta
    25201 ttattgcaac agcgaaatat ccgcaacgcg caggaacagt tcgcgcagtt tctccagcat
    25261 ggtcagacgg ttgatacgca attctttgtc atcaaccatg accatcactt tatcgaagaa
    25321 agcatcaacc ggttcacgca gctcagccag ttcgaccagc gcatcctggt aacgaccttc
    25381 cgtaaagtac ggctccagct tgtcacgtag caccacaacc tgcatcgcca gtttaatttc
    25441 ttccggctct ttcagggtag aggcattcac gcggtcgctc agcacttcgt cagatttcgc
    25501 cagaatgtta gatacacgtt tgttcgccgc cgccagtgca gcagctgcat ccagggtacg
    25561 gaaatgcgat accgctttca tacgggcatc gaaatcagcc ggacgagtcg gacgacgcgc
    25621 cagtaccgcc tggatggtgt caacggtata accttcgtcc tgataccagg cgcggaagcg
    25681 accgagcata aagtcgataa catcatcaac tacgttggca ttagtcagct tatcgccata
    25741 cagacgcacc gcttcttcgg tcagcgtttg cagatcaagg ttgaggttct tctcaacgat
    25801 aattcgcagc acgccaagcg cggcacgacg cagcgcaaac gggtctttgt cgcctttcgg
    25861 atgctgaccg ataccgaaga tacccgccag ggtatccatc ttgtcagcaa tcgccagcgc
    25921 acaagctact gggttggacg gcaggtcatc accagcaaaa cgcggctgat actgctcatt
    25981 cagcgccacc gcgacatctt ccgcttcgcc atcgtgacgc gcatagtgca tccccataac
    26041 gccctgggtg tcggtgaact cgaagaccat gttggtcatt aggtcgcact tagacagcag
    26101 acccgcacgg gtagcgtggt taacgtcagc gccaatctgt tcagcaatcc agccagccag
    26161 cgcctggatg cggtcagttt tgtcgcgcag cgtccccaac tgttgctgga acaacacggt
    26221 ttgcaggcgc ggcaggttat cttcaagacg ttttttacgg tcggtgttga agaagaactc
    26281 ggcatccgcc agacgcggac gaacgacttt ctcgttaccg gagataatct gctgcggatc
    26341 tttcgattcg atgttggcaa cgaagataaa gttcggcagc agtttgccgt cgttcgcata
    26401 caccgggaag tatttctggt cacctttcat ggtgtaaacc agcgcttcag ccggcaccgc
    26461 gaggaatttc tcttcgaatt ttgcggtcag aacgaccggc cactccacca gcgaagccac
    26521 ttcttccagc aggctttcgc ttaagtcagc gttaccgcca atcttacgcg ctgcttcttc
    26581 ggcatcggct ttaatcttcg ccttacgttc ttcgtaatcg gcgatgactt tcccacgctc
    26641 acgcagaatt tccggatact gatcggcgtt atcgatggtg aattccggct cgcccataaa
    26701 gcggtggccg cgaatcacgc gatcggactg aatgcccaga atggttgccg gaatgacttt
    26761 gtcgcccagc agcagggtca cggtgtgcac cggacgcacg aagtgcacgt cgcttgcgcc
    26821 ccaacgcatc agtttcggga tcggcagttt cgccagagaa gtcgcaacca tattcggcag
    26881 cagtgcttcg gtgctttcgc ccttcacatg ggcgcgatac agcagccatt cgcctttatc
    26941 ggtagtcaga cgctcagcct ggtcaacggt aataccgcaa ccacgcgccc aaccttctgc
    27001 cgctttgctc ggtttgcctt cagcgtcgaa cgcctgggca atcgccgggc cgcgtttttc
    27061 gatttcacga tccggttgcg cttccgccag gttagctact ttcagcgcca gacgacgcgg
    27121 agcagcaaac cattgaacgg tgccgtgtgc gaggccagcg ttatccagct ccgcagtaaa
    27181 gttcgcagca aaggactcag ccaggctgcg cagtgctttt ggtggcagct cttcagtgcc
    27241 gatttccacc agaaaagttt tctcagacat agccgcctct tacttatctt tgttgcacat
    27301 cgggaagccg agggcttcac gggaagcgta gtatgcttct gccactgctt tggtcagggt
    27361 gcgaatgcgc agaatgtagc gctgacgctc ggtgacggag atggctttac gcgcatccag
    27421 caggttgaag ctgtgggcgg ctttcagaat acgctcgtag gctggcagcg gcagcggatt
    27481 ttccagcgcc agcagctgct gcgcttcttt ctcgtactgc tcgaagcagg tgaacaggaa
    27541 gtccacatcc gcgtattcga agttgtaagt ggactgctcc acttcgttct gatggaacac
    27601 gtcgccgtag gtggttttac ccagcgggcc gtcgctccag accaggtcgt aaacgctgtc
    27661 tacgccctga atgtacatgg ccagacgttc cagaccgtag gtgatctcgc cggtaaccgg
    27721 tttacactcc agaccaccaa cctgctggaa gtaagtgaac tgcgtcactt ccatgccgtt
    27781 cagccacact tcccagccca gtccccaggc acccagcgtc gggttttccc agttatcttc
    27841 cacgaaacgg atgtcgtgaa tagtcgggtc catgcccagc tctttcagag aaccgaggta
    27901 cagctcctga atattgtccg gcgatggctt aatgaccacc tggaactgat agtagtgctg
    27961 taaacggttg gggttttcgc cgtagcgacc atcggtcggg cgacgagaag gctgaacata
    28021 agcagccgcc atcggttctg gccccagcgc gcgcagacag gtcattgggt gagaggttcc
    28081 cgcgccgact tccatgtcca atggttgaac aatggtgcag ccctggcgag cccagtaatc
    28141 ctgtaaggtc aggatcaagc cctggaaggt cctggtatca aacttttgca tattatttcg
    28201 tgctggatac gtgtggattt aaaggaaggg atcagtatac ccgctggatg gaagatatac
    28261 agtacgaaac gggaaaaagc agggcttaac gcatggaaag atgcaaaaaa tggccatccg
    28321 cgtcaaaaga acagacaaac tgctctttac ggaaggtata accgcgcatt tcatagcttc
    28381 cctggaactg ttcgaaggcg gtgacgtcga ttttttgctc gccggtgtta taacgttttg
    28441 ccgcctgatc cttgcataac gcttccatat tcaacgaacg cagaggatca acctttaccc
    28501 gttgtgcttt ctgcacgggt gacggtatag aacaacccac tagcaccata agcgccattg
    28561 ccggaaagaa agcgttcatc atcattaaat taccgtctgg tttgcaggtg agtcttatta
    28621 tttatatcgg tatagtggct gatatcttac gaatttcctt aagcctctat aagctgtagt
    28681 gataaaactc agattattcc agtgcgccaa ccaaatgatt tatcggtcag acttttttta
    28741 accagataca gagacacaga tgcagcccaa aatttactgg attgataacc tgcgagggat
    28801 agcgtgttta atggtggtga tgattcacac cactacctgg tatgtgacca atgctcatag
    28861 tgttagcccc gtcacatggg atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt
    28921 gccgctattt ttcatgattt ccggctatct cttttttggc gaacgcagcg cccagccgcg
    28981 ccatttcttg cgtatcggct tatgtctgat tttttatagc gcaatcgcac tgctctacat
    29041 tgcgctgttt acctccatca atatggagtt agcgctgaaa aacctgctgc aaaagccagt
    29101 gttttaccac ttgtggtttt tcttcgcgat tgcggtgatt tatctggttt caccgctgat
    29161 tcaggtgaag aacgtcggcg gaaaaatgtt gctggtgcta atggcggtga ttggcattat
    29221 cgctaaccca aacacagtgc cgcagaaaat tgacggtttt gaatggctgc caattaactt
    29281 atatatcaat ggcgatactt tttactacat tctgtatggc atgttgggcc gcgctatagg
    29341 gatgatggac acacagcata aagcactgtc gtgggtgagc gccgcgctgt ttgcgacggg
    29401 ggtttttatt atctctcgcg ggacattata tgaattgcag tggcgcggaa attttgccga
    29461 tacctggtat ctttactgtg ggccgatggt ttttatctgc gcaatcgcgc tattgactct
    29521 ggttaaaaac acgctggata cgcgtaccat tcgcggactt ggcttaatct cccgccattc
    29581 attgggtata tacggattcc acgccttgat tatccatgcg ctgcgcaccc ggggaattga
    29641 gcttaaaaat tggccaatac tggatattat ttggatcttt tgcgcgacgt tggcagcgag
    29701 tttgttactt tctatgctgg tacaacgaat cgacagaaac agattagtga gttaagtaaa
    29761 agcccggtca cattggactg accgggctta cgtgagttat tcgctgtatt cttcctgggt
    29821 aacctgtgtt tcttttggtg ggtttattcc ggcatcacga atatttttct gcaccgctac
    29881 tgcaccaaac aagcttaaga agaaagccag tccataaaaa cctttttcgc tgagtaataa
    29941 tgtcgcattc cacagaccta ccatcagtaa tgcaacagag ataataaaga cagtcaggca
    30001 ggtcatataa taaatggaag tggtcggtat gccttcatac ttgtcccgaa cggtcttttg
    30061 ataagacgcc gcagaaaaca gtcctaatac cagtacggca aaataatatc ctttttcatt
    30121 taactgcatc tctgcattcc atagccctaa cagataggta acgataccac caacgagagc
    30181 tatccatgac acaatactaa aggccggtga ataggttgat attttgttgt ccatcacagt
    30241 attcctttca ttcctgaata tgtaagagct ttatgttgct catgccgagc gaaacaaacg
    30301 catttgacca aacaagcaga caaaaaaggc gatgggatag atcaccattt cataagtatt
    30361 gataggtgcg tttaaaacac cgataaacaa gagaccgata gtgattaacg caactaactg
    30421 gcagatatgg gtaaaaaaat catccagttg cccgagtttt tctgcgcgaa gatatgcata
    30481 gttgccaaaa gttgctgtca ttaacacgcc aagaaaatag ccttttccac ttaacaatgg
    30541 gcatgagatc catagcccaa ccagataaac cagcgccccg acaacaaata atagttttgc
    30601 cacagtcttt gaggttttca taacgatctc catatctacc agcgatacat tacgagtaac
    30661 caacgaaaga caaaactgaa aaatgccatt aacaaatgat tttcagaata aattcatact
    30721 aaatattaat taattactga gatatataga tgtgaattat cccccacccg gtcaggcagg
    30781 ggataacgtt tacgccatta atggcagaag ttgctgatag aggcgacgga acgtttctcg
    30841 tcgtggctga taagcggcat aacgctgcgc atctggtaga tgcgactgtt ctaacggtag
    30901 ttgcggcaac aattcaatga gcgatttctc tggattcgcc gcgatctgcg ccagccttgc
    30961 tgcgcccagt gctggcccca catccccccc cgtacggtaa tcgagctgct gaccgctgat
    31021 atccgccagc atctgacgcc agtactcact acgcgccccg cccccaatca acgtaacact
    31081 ttgcggttta ataccgcagg catgcacgac atccatgcca tctgccagcg cataacccac
    31141 gccttccagc actgctcgcg ccagttcatt ggggccatgt tgatgagtca aaccaaagaa
    31201 aacccccttc gcctggggat tattgtgtgg cgtacgctcg ccggaaagat aaggcagaaa
    31261 ccaaactggc tcggcacttt catcagcctg ttgagctgca gcgattaaag ctgggacatt
    31321 gctcaggccg gttaatttcg cggcccaatc cagacacgac gctgcactca gcatcacaga
    31381 cattaaatgc caacgttgcg gtagcgcatg gcaaaagcta tgtacggcgc tttctggctt
    31441 gcttaagaac ccttcgctga cagcaaaata gacccccgac gtccccagcg ataacattgc
    31501 ctgattagca tcaaccattc ccacaccaac tgcaccagct gcattgtcgc caccgcctgc
    31561 gacaactggc accgtcgcca taccccacgc tttcgcaact tcaggtaaca aagcaccagt
    31621 aatttcgctg ccttcgtata atgcgggcat ctggtcacga gataagtcgc aagcctgcag
    31681 catgacgtca ctccagtcac gctttgcgac atccagccac atggtgccag ctgcgtcaga
    31741 catatcgctg gcaaactccc ccgtcatacg cagacgcaag taatctttcg gtaataatac
    31801 tttgtcgatt tgacggaata tctccggctc atgccgctga acccatagca atttaggcgc
    31861 agtaaatccg ggcatcatca ggttgccggt aatcacccgc gattgcggaa ctcgcgcttc
    31921 cagcaaagtg cactcttgcg cacagcgccc gtcgttccac aaaatggcag ggcgtaacac
    31981 ccgttgctga gcatccagca aggttgctcc gtgcatctgg ccggcaatac ccaatgcttt
    32041 aacgtcctgc agagaatgct gatcgcccag agctttcatt gcgcgatcag ttgcctgcca
    32101 ccactgttcc gggtcttgtt ccgaccagag tggatgcggg cgcgaaacgg tcagcttttc
    32161 cgtttgcgca gcaaccacct caccctgctc gttgagcaaa ataactttta cgcccgaggt
    32221 gccaagatct atcccgatat acatatcgat cgttccttaa aaaaatgccc ggtatcgcta
    32281 ccgataaccg ggccaacgga ctgcacagtt agccgttatt tgtcgaacag ataatggttt
    32341 accagatttt ccagttgttc ctggcgacca ctctgatgca ccggagacaa atgatgttcc
    32401 tgagcatatt tggctaaatc tgccagtgac atttggcctt tcaggatttg ctggcccaat
    32461 tcgctattcc agccggaata acgctgcgcg atgcgtttat ccagctcgcc atcttcaatc
    32521 atgcgcgctg caattttcag cgccagtgcc atcgtatcca tcgcgccgat atgaccgtaa
    32581 aacagatcat atttatcagt actttgacga cgtactttgg catcgaagtt cagaccaccg
    32641 gtggtgaaac cgcctgcttt gagaatttca tacatcacca gcgcattctc ttccacactg
    32701 ttcgggaact ggtcggtgtc ccagcccagt tgcgcatcgc cacggttggc gtcgacagaa
    32761 ccgaacaggc caagcgcaat ggcggtggct atttcatgat ggaaagagtg acctgccagc
    32821 gtcgcgtggt tagcttcaat gttcagttta atctcttttt ccagaccaaa ctgtttcagg
    32881 aagccataga ccgtcgcggc atcgtaatca tattgatgtt tggtcggttc ttgcggtttc
    32941 ggttcgataa gcaacgtgcc ctggaaaccg attttatgtt tatgctcaac caccatctgc
    33001 ataaagcggc ccagttgttc acgctcctga cgcaagtcgg tatttaacag cgtttcgtaa
    33061 ccttcacgac cgccccacag gacatagttt tcaccgccca atttatgggt tgcttccatc
    33121 gctgtaacaa cttgcgttgc cgcccagctg aagacttcag gatctgggtt cgtcgccgca
    33181 cccgcgccgt agcgagggtt tgtaaagcag ttggcggttc cccacagcag cttcacgccg
    33241 ctctcttctt gcttgcctgc caggacatca accatttgcg caaaattatt gatgtactct
    33301 tttaacgacg cgccctcagg ggaaacatcc acatcgtgga agcaataaaa tggcacatgt
    33361 aacttgtgga aaaactcaaa tgcgacatct gctttacgct tcgccaacgc cagtgcctca
    33421 ccaggctgct gccacggacg attaaacgcc cccacaccaa acatatccgc cccgttccag
    33481 cagaaggtgt gccagtagca ggcggcaaaa cgcaagtgct cttccatacg cttacccaac
    33541 accagttcgt cgggattgta gtgacggaat gctaacgggt ttgaggattt tgagccttca
    33601 taacgaacgc gatcgagctg gtcaaaatag gcttgcatat tgaactccat aatcaggtaa
    33661 tgccgcgggt gatggatgat gtcgtaatat tgggcactcc ctttcagttg ctcaattatg
    33721 ttatttcaca ctgctattga gataattcac aagtgtgcgc tcgctcgcaa aataaaatgg
    33781 aatgatgaaa ctgggtaatt cctcgaagag aaaaatgcaa taagtacaat tgcgcaacaa
    33841 aagtaagatc tcggtcataa atcaagaaat aaaccaaaaa tcgtaatcga aagataaaaa
    33901 tctgtaattg ttttcccctg tttagttgct aaaaattggt tacgtttatc gcggtgattg
    33961 ttacttatta aaactgtcct ctaactacag aaggccctac accatgaaaa taaagaacat
    34021 tctactcacc ctttgcacct cactcctgct taccaacgtt gctgcacacg ccaaagaagt
    34081 caaaataggt atggcgattg atgatctccg tcttgaacgc tggcaaaaag atcgagatat
    34141 ctttgtgaaa aaggcagaat ctctcggcgc gaaagtattt gtacagtctg caaatggcaa
    34201 tgaagaaaca caaatgtcgc agattgaaaa catgataaac cggggtgtcg atgttcttgt
    34261 cattattccg tataacggtc aggtattaag taacgttgta aaagaagcca aacaagaagg
    34321 cattaaagta ttagcttacg accgtatgat taacgatgcg gatatcgatt tttatatttc
    34381 tttcgataac gaaaaagtcg gtgaactgca ggcaaaagcc ctggtcgata ttgttccgca
    34441 aggtaattac ttcctgatgg gcggctcgcc ggtagataac aacgccaagc tgttccgcgc
    34501 cggacaaatg aaagtgttaa aaccttacgt tgattccgga aaaattaaag tcgttggtga
    34561 ccaatgggtt gatggctggt taccggaaaa cgcattgaaa attatggaaa acgcgctaac
    34621 cgccaataat aacaaaattg atgctgtagt tgcctcaaac gatgccaccg caggtggggc
    34681 aattcaggca ttaagcgcgc aaggtttatc agggaaagta gcaatctccg gccaggatgc
    34741 ggatctcgca ggtattaaac gtattgctgc cggtacgcaa actatgacgg tgtataaacc
    34801 tattacgttg ttggcaaata ctgccgcaga aattgccgtt gagttgggca atggtcagga
    34861 accaaaagca gataccacac tgaataatgg cctgaaagat gtcccctccc gcctcctgac
    34921 accgatcgat gtgaataaaa acaacatcaa agatacggta attaaagacg gattccacaa
    34981 agagagcgag ctgtaagcgt tacgccccag cgcggagcgg gggcgtgatt tctctccatg
    35041 ccgcgtgaat gaattggctt aggtggagtc gttatgcctt atctacttga aatgaagaac
    35101 attaccaaaa ccttcggcag tgtgaaggcg attgataacg tctgcttgcg gttgaatgct
    35161 ggcgaaatcg tctcactttg tggggaaaat gggtctggta aatcaacgct gatgaaagtg
    35221 ctgtgtggta tttatcccca tggctcctac gaaggcgaaa ttatttttgc gggagaagag
    35281 attcaggcga gtcacatccg cgataccgaa cgcaaaggta tcgccatcat tcatcaggaa
    35341 ttggccctgg tgaaagaatt gaccgtgctg gaaaatatct tcctgggtaa cgaaataacc
    35401 cacaatggca ttatggatta tgacctgatg acgctacgct gtcagaagct gctcgcacag
    35461 gtcagtttat ccatttcacc tgatacccgc gttggcgatt tagggcttgg gcaacaacaa
    35521 ctggttgaaa ttgccaaggc acttaataaa caggtgcgct tgttaattct cgatgaaccg
    35581 acagcctcat taactgagca ggaaacgtcg gttttactgg atattattcg cgatctacaa
    35641 cagcacggta tcgcctgtat ttatatttcg cacaaactca acgaagtcaa agcgatttcc
    35701 gatacgattt gcgttattcg cgacggacag cacattggta cgcgtgatgc tgccggaatg
    35761 agtgaagacg atattatcac catgatggtc ggtcgagagt taaccgcgct ttaccctaat
    35821 gaaccacata ccaccggaga tgaaatatta cgtattgaac atctgacggc atggcatccg
    35881 gttaatcgtc atattaaacg agttaatgat gtctcgtttt ccctgaaacg tggcgaaata
    35941 ttgggtattg ccggactcgt tggtgccgga cgtaccgaga ccattcagtg cctgtttggt
    36001 gtgtggcccg gacaatggga aggaaaaatt tatattgatg gcaaacaggt agatattcgt
    36061 aactgtcagc aagccatcgc ccaggggatt gcgatggtcc ccgaagacag aaagcgcgac
    36121 ggcatcgttc cggtaatggc ggttggtaaa aatattaccc tcgccgcact caataaattt
    36181 accggtggca ttagccagct tgatgacgcg gcagagcaaa aatgtattct ggaatcaatc
    36241 cagcaactca aagttaaaac gtcgtccccc gaccttgcta ttggacgttt gagcggcggc
    36301 aatcagcaaa aagcgatcct cgctcgctgt ctgttactta acccgcgcat tctcattctt
    36361 gatgaaccca ccaggggtat cgatattggc gcgaaatacg agatctacaa attaattaac
    36421 caactcgtcc agcagggtat tgccgttatt gtcatctctt ccgaattacc tgaagtgctc
    36481 ggccttagcg atcgtgtact ggtgatgcat gaagggaaac taaaagccaa cctgataaat
    36541 cataacctga ctcaggagca ggtgatggaa gccgcattga ggagcgaaca tcatgtcgaa
    36601 aagcaatccg tctgaagtga aattggccgt accgacatcc ggtggcttct ccgggctgaa
    36661 atcactgaat ttgcaggtct tcgtgatgat tgcagctatc atcgcaatca tgctgttctt
    36721 tacctggacc accgatggtg cctacttaag cgcccgtaac gtctccaacc tgttacgcca
    36781 gaccgcgatt accggcatcc tcgcggtagg aatggtgttc gtcataattt ctgctgaaat
    36841 cgacctttcc gtcggctcaa tgatggggct gttaggtggc gtcgcggcga tttgtgacgt
    36901 ctggttaggc tggcctttgc cacttaccat cattgtgacg ctggttctgg gactgcttct
    36961 cggtgcctgg aacggatggt gggtcgcgta ccgtaaagtc ccttcattta ttgtcaccct
    37021 cgcgggcatg ttggcatttc gcggcatact cattggcatc accaacggca cgactgtatc
    37081 ccccaccagc gccgcgatgt cacaaattgg gcaaagctat ctccccgcca gtaccggctt
    37141 catcattggc gcgcttggct taatggcttt tgttggttgg caatggcgcg gaagaatgcg
    37201 ccgtcaggct ttgggtttac agtctccggc ctctaccgca gtagtcggtc gccaggcttt
    37261 aaccgctatc atcgtattag gcgcaatctg gctgttgaat gattaccgtg gcgttcccac
    37321 tcctgttctg ctgctgacgt tgctgttact cggcggaatg tttatggcaa cgcggacggc
    37381 atttggacga cgcatttatg ccatcggcgg caatctggaa gcagcacgtc tctccgggat
    37441 taacgttgaa cgcaccaaac ttgccgtgtt cgcgattaac ggattaatgg tagccatcgc
    37501 cggattaatc cttagttctc gacttggcgc tggttcacct tctgcgggaa atatcgccga
    37561 actggacgca attgcagcat gcgtgattgg cggcaccagc ctggctggcg gtgtgggaag
    37621 cgttgccgga gcagtaatgg gggcatttat catggcttca ctggataacg gcatgagtat
    37681 gatggatgta ccgaccttct ggcagtatat cgttaaaggt gcgattctgt tgctggcagt
    37741 atggatggac tccgcaacca aacgccgttc ttgattttga taaaaatttt ctcaaagccg
    37801 gttacgtatt accggttttg agtttttgca tgattcagca ggaaaagaac catgtttact
    37861 aaacgtcacc gcatcacatt actgttcaat gccaataaag cctatgaccg gcaggtagta
    37921 gaaggcgtag gggaatattt acaggcgtca caatcggaat gggatatttt cattgaagaa
    37981 gatttccgcg cccgcattga taaaatcaag gactggttag gagatggcgt cattgccgac
    38041 ttcgacgaca aacagatcga gcaagcgctg gctgatgtcg acgtccccat tgttggggtt
    38101 ggcggctcgt atcaccttgc agaaagttac ccacccgttc attacattgc caccgataac
    38161 tatgcgctgg ttgaaagcgc atttttgcat ttaaaagaga aaggcgttaa ccgctttgct
    38221 ttttatggtc ttccggaatc aagcggcaaa cgttgggcca ctgagcgcga atatgcattt
    38281 cgtcagcttg tcgccgaaga aaagtatcgc ggagtggttt atcaggggtt agaaaccgcg
    38341 ccagagaact ggcaacacgc gcaaaatcgg ctggcagact ggctacaaac gctaccaccg
    38401 caaaccggga ttattgccgt tactgacgcc cgagcgcggc atattctgca agtatgtgaa
    38461 catctacata ttcccgtacc ggaaaaatta tgcgtgattg gcatcgataa cgaagaactg
    38521 acccgctatc tgtcgcgtgt cgccctttct tcggtcgctc agggcgcgcg gcaaatgggc
    38581 tatcaggcgg caaaactgtt gcatcgatta ttagataaag aagaaatgcc gctacagcga
    38641 attttggtcc caccagttcg cgtcattgaa cggcgctcaa cagattatcg ctcgctgacc
    38701 gatcccgccg ttattcaggc catgcattac attcgtaatc acgcctgtaa agggattaaa
    38761 gtggatcagg tactggatgc ggtcgggatc tcgcgctcca atcttgagaa gcgttttaaa
    38821 gaagaggtgg gtgaaaccat ccatgccatg attcatgccg agaagctgga gaaagcgcgc
    38881 agtctgctga tttcaaccac cttgtcgatc aatgagatat cgcaaatgtg cggttatcca
    38941 tcgctgcaat atttctactc tgtttttaaa aaagcatatg acacgacgcc aaaagagtat
    39001 cgcgatgtaa atagcgaggt catgttgtag cgatgatgag aattgtcggc gtcacatcag
    39061 gtaacgctgc gtggttgtcg gatgcggcgt gaacgcctta tccgacctac ccgccaggca
    39121 tgataaaacg caccaacaac gcttcaggct cgtagctcaa ctgcctgaga caaagtaaaa
    39181 agccttatcc gactgacaag tcggataagg ctcaaggaaa tgcaattaca tatgcgccgc
    39241 gattaaccgt tggttatcct ggtacattgc gaacaggtag ttgttataac tcttcccttt
    39301 ggtcgagtag cccttcagct tgtgaatcat cgctgtggca gtcacttcct gatccgcttt
    39361 acgcagctgc gcacgcgatt tacggaacga agagtaagcc gggtgcgtat tcaggttagt
    39421 gacataggcg ctcaccgatt ctttgacaga actaaactgt gagtaccctt tcactttacc
    39481 tggcgcattg gtacaacgtc ctttcatgca tttcatgccg aacaggttgt tgttgttgcg
    39541 cgccagcttc gacgttcccc aaccgctttc tgctgcagcc atcgtcgcca ccatactggt
    39601 ggggataatg tctacgcgtt caagcaaggt attccacggg atttttcgcg tattaccgga
    39661 ccacttcacc ttgtagcgtt tggcgatgtc tttcagacgc gcacgctcag caggtgacca
    39721 ttggccctga tactgttttg aaatgagcca gttacgttcc acagtaatgg ccgcattttg
    39781 gctggtaatg taaggcatta cggtccggag aaacgccttt ttccttggtg ttccggaagg
    39841 gtattttcgc aaatcaggaa gtgaactact ctttgcacta ttgcgagaat actcttgttt
    39901 actgcttacc tgtttattac tagctttagt taagtgggac ttttgactcg ctgttgttgt
    39961 gtgcgtcttc gctaacacct cactcgaaaa caccagagtg agtaacataa gaatcatcgc
    40021 cccatatcgt cgtatgggag tcaaaatcat caggtctcct ggtcggattt aatcattcca
    40081 acaccttata tttttcacaa atttgagagt tgaatctcaa atcatatcaa aaatagctgt
    40141 caagagcacc ccaaggaata gtccaaatct gaaactatgt cacgtgttaa cgattcagat
    40201 tggcgctaaa tcgcagaaaa tgtgggggtt atcgcaaaat tcagccgttt tttgcgcgag
    40261 atcgctcacc cttgcttctc atcctgtgga cttaccgctc agggatgagt tttgtttggc
    40321 ttatcgctgg caaactgtct gaaatcgcag caataaggac tcatccgcca tgaaactcgc
    40381 cgcctgtttt ctgacactcc ttcctggctt cgccgttgcc gccagctgga cttctccggg
    40441 gtttcccgcc tttagcgaac aggggacagg aacatttgtc agccacgcgc agttgcccaa
    40501 aggtacgcgt ccactaacgc taaattttga ccaacagtgc tggcagcctg cggatgcgat
    40561 aaaactcaat cagatgcttt ccctgcaacc ttgtagcaac acgccgcctc aatggcgatt
    40621 gttcagggac ggcgaatata cgctgcaaat agacacccgc tccggtacgc caacattgat
    40681 gatttccatc cagaacgccg ccgaaccggt agcaagcctg gtccgtgaat gcccgaaatg
    40741 ggatggatta ccgctcacag tggatgtcag cgccactttc ccggaaggag ccgccgtacg
    40801 ggattattac agccagcaaa ttgcgatagt gaagaacggt caaataatgt tacaacccgc
    40861 tgccaccagc aacggtttac tcctgctgga acgggcagaa actgacacat ccgccccttt
    40921 cgactggcat aacgccacgg tttactttgt gctgacagat cgtttcgaaa acggcgatcc
    40981 cagtaatgac cagagttacg gacgtcataa agacggtatg gcggaaattg gcacttttca
    41041 cggcggcgat ttacgcggcc tgaccaacaa actggattac ctccagcagt tgggcgttaa
    41101 tgctttatgg ataagcgccc catttgagca aattcacggc tgggtcggcg gcggtacaaa
    41161 aggcgatttc ccgcattatg cctaccacgg ttattacaca caggactgga cgaatcttga
    41221 tgccaatatg ggcaacgaag ccgatctacg gacgctggtt gatagcgcac atcagcgcgg
    41281 tattcgtatt ctctttgatg tcgtgatgaa ccacaccggc tatgccacgc tggcggatat
    41341 gcaggagtat cagtttggcg cgttatatct ttctggtgac gaagtgaaaa aatcgctggg
    41401 tgaacgctgg agcgactgga aacctgccgc cgggcaaacc tggcatagct ttaacgatta
    41461 cattaatttc agcgacaaaa caggctggga taaatggtgg ggaaaaaact ggatcagaac
    41521 ggatatcggc gattacgaca atcctggatt cgacgatctc actatgtcgc tagccttttt
    41581 gccggatatc aaaaccgaat caactaccgc ttctggtctg ccggtgttct ataaaaacaa
    41641 aatggatacc cacgccaaag ccattgacgg ctatacgccg cgcgattact taacccactg
    41701 gttaagtcag tgggtccgcg actatgggat tgatggtttt cgggtcgata ccgccaaaca
    41761 tgttgagttg cccgcctggc agcaactgaa aaccgaagcc agcgccgcgc ttcgcgaatg
    41821 gaaaaaagct aaccccgaca aagcattaga tgacaaacct ttctggatga ccggtgaagc
    41881 ctggggccac ggcgtgatgc aaagtgacta ctatcgccac ggcttcgatg cgatgatcaa
    41941 tttcgattat caggagcagg cggcgaaagc agtcgactgt ctggcgcaga tggatacgac
    42001 ctggcagcaa atggcggaga aattgcaggg tttcaacgtg ttgagctacc tctcgtcgca
    42061 tgatacccgc ctgttccgtg aagggggcga caaagcagca gagttattac tattagcgcc
    42121 aggcgcggta caaatctttt atggtgatga atcctcgcgt ccgttcggtc ctacaggttc
    42181 tgatccgctg caaggtacac gttcggatat gaactggcag gatgttagcg gtaaatctgc
    42241 cgccagcgtc gcgcactggc agaaaatcag ccagttccgc gcccgccatc ccgcaattgg
    42301 cgcgggcaaa caaacgacac ttttgctgaa gcagggctac ggctttgttc gtgagcatgg
    42361 cgacgataaa gtgctggtcg tctgggcagg gcaacagtaa cttttccggc ttcccgttcg
    42421 tcagtacctc gggaagccgc caaccaggat aaaatgtcag ccctaatcag cgttgcagga
    42481 taaagcaccg ctcactcttc aacagaccga tttgcacccc agcaaatgta gcgttattgt
    42541 taccttcctt gctacagagt tcgacagata tcccgctatg acattctccc tttttggtga
    42601 caaatttacc cgccactccg gcattacgct gttgatggaa gatctgaacg acggtttacg
    42661 cacgcctggc gcgattatgc tcggcggcgg taatccggcg cagatcccgg aaatgcagga
    42721 ctacttccag acgctactga ccgacatgct ggaaagtggc aaagcgactg atgcactgtg
    42781 taactacgac ggtccacagg ggaaaacgga gctactcaca ctgcttgccg gaatgctgcg
    42841 cgagaagttg ggttgggata tcgaaccaca gaatattgca ctaacaaacg gcagccagag
    42901 cgcgtttttc tacttattta acctgtttgc cggacgccgt gccgatggtc gggtcaaaaa
    42961 agtgctgttc ccgcttgcac cggaatacat tggctatgct gacgccggac tggaagaaga
    43021 tctgtttgtc tctgcgcgtc cgaatattga actgctgccg gaaggccagt ttaaatacca
    43081 cgtcgatttt gagcatctgc atattggcga agaaaccggg atgatttgcg tctcccggcc
    43141 gacgaatcca acaggcaatg tgattactga cgaagagttg ctgaagcttg acgcgctggc
    43201 gaatcaacac ggcattccgc tggtgattga taacgcttat ggcgtcccgt tcccgggtat
    43261 catcttcagt gaagcgcgcc cgctatggaa tccgaatatc gtgctgtgca tgagtctttc
    43321 caagctgggt ctacctggct cccgctgcgg cattatcatc gccaatgaaa aaatcatcac
    43381 cgccatcacc aatatgaacg gcattatcag cctggcacct ggcggtattg gtccggcgat
    43441 gatgtgtgaa atgattaagc gtaacgatct gctgcgcctg tctgaaacag tcatcaaacc
    43501 gttttactac cagcgtgttc aggaaactat cgccatcatt cgccgctatt taccggaaaa
    43561 tcgctgcctg attcataaac cggaaggagc cattttcctc tggctatggt ttaaggattt
    43621 gcccattacg accgagcagc tctatcagcg cctgaaagca cgcggcgtgc tgatggtgcc
    43681 ggggcacaac ttcttcccag ggctggataa accgtggccg catacgcatc aatgtatgcg
    43741 catgaactac gtaccagagc cggagaaaat tgaggcgggg gtgaagattc tggcggaaga
    43801 gatagaaaga gcctgggctg aaagtcacta aaaattgcct gatgcgctac gcttatcagg
    43861 cctacatgat ttctgcaata tattgaattt ggaagaattt gtaggccgga taaggcgttt
    43921 acgccgcatc tggcataaac aaagcgcact gttccggcgt tgagaaacgc cggaaaacgt
    43981 ttcaaacagg ctgctgccgt agccgatgcc gctgcacttt ctcgacatcc atgcactgca
    44041 acgcctttgt cgggcaggct tcaacacagg ccggtcccgt ctcccgatgc cagcacagat
    44101 cgcacttaat cgccctcgcc tttttgcgcg aagaaacgac ctccatcgca ccaaacgggc
    44161 aagccagcat acagctttta cagccaatgc aacgtgtttg ttcaacgaaa atatgcccat
    44221 gttcgcggct tatcgcgtca acagggcaga cattcgcgca cggtgcatct tcacactgat
    44281 gacaggctac tgccgtggtc cagcagtggt ctttaatgac acgaatacgg gaaataaact
    44341 cgtctggtga caacgcagcg caatcctgat tctcatgatg cgacactgcg caagccactt
    44401 cacaggtacg gcaaccgata catttcgtcg catccgcaat aataaaccgg ttcatgatca
    44461 ctcctttgca ctaaaacagc agagtgccag agagcgcgtc cctactgctt tgatccaacg
    44521 aaggaaatgt taaagttttg gcacttgtcc aaagcgttat gtgatcgcgc ccaggtcatc
    44581 cctgacagta aatcccagtt cattagaaat agcctgcgcg gtttcacgca gtggtttcag
    44641 gagatttttc tctcccacct gtttcagacg tgatgtcgaa agcgaaatcg acacggcgta
    44701 cggcacccgc ccatgaatat caaacaccgg aacagcaata caggagacgc cgagttcgtt
    44761 ttcttctctg tccatcgccg ctccgctttc acgaatgtgc gccagttcgt cgaacatcgc
    44821 gggcagctcg gtaatggtat tgcgggttaa cggctggatc tcatgctgat ggctttccca
    44881 gtatgacttc acgtagtccg ggtgaccaaa cgccatgtag atcttgccca ttgcggaaca
    44941 gtagagcggc atatgctggc caatataggc acgggttcgc agcatcccgg ttgtgggttc
    45001 cagcttataa atcaaaatag cgtgatcgtc ttcgcggctg gagaagttaa tggtttcacc
    45061 agtggcgatg ttcagtgcct caagatgcgg agcggcgata tgaatgatat tcagcgaaga
    45121 cagcgccttc tgcccgacgg caataaattt ggtggtcagg cgataactcc ctgcggcagg
    45181 cgcggtggtc acatagccgc aggactgtaa tccctgcaat aagcgatgga cggtactctt
    45241 atttaaacca gccagctccg aaagatgcgc caacggacaa ccgtttggat agttgctcaa
    45301 aatctcaatc agcatcaacc cgcgaaacag actctggctt ccggctggac gctctttttc
    45361 ctgcgccatc tcgttctctt tttttcccat cacttctttc cccattttgt cgcgtcctga
    45421 tggtagcgca aagtgtgccg tagttcacga tctcgacaga taatttataa ccaattgatt
    45481 tttatgtctt ttgaaattca tcaatcagat tgctttgtta aaaagtgatc gatatatttg
    45541 aaatcaagtt tcgcatattg aaattttaag ccaaaaaagc gatcaaaaaa acaaggaagc
    45601 ctgggatgaa agtgacattt gagcagttaa aagcagcctt taatcgggtc ttaatttcac
    45661 gcggcgttga cagcgaaacg gctgacgcct gtgcagagat gttcgcccgc accaccgaat
    45721 ccggcgttta ttctcacggc gttaatcgtt tccctcgttt cattcaacaa ctggaaaacg
    45781 gcgatatcat tcctgatgcc caacccaaac gtataaccag cctcggcgca attgaacagt
    45841 gggacgccca gcgttcgatc ggtaacctga cagcgaaaaa gatgatggat cgcgccattg
    45901 aactggctgc cgatcacggt attggtctgg tggcactacg taatgccaac cactggatgc
    45961 gcggcggcag ctacggctgg caggcggcgg aaaaaggcta tattggcatt tgctggacca
    46021 actccatcgc cgtaatgccg ccgtggggcg caaaagagtg tcgcatcggc actaacccgc
    46081 tgatcgtcgc cattccttcc acgccgatca ccatggtcga tatgtcgatg tcgatgttct
    46141 cttacggcat gttagaagtt aaccgtctgg caggtcgtca gctcccggtc gatggtggct
    46201 ttgatgatga gggcaatttg accaaagaac ctggcgttat cgagaagaat cgccgcattt
    46261 tgccgatggg ctactggaaa ggttctggca tgtcgattgt gctggatatg atcgctactc
    46321 tcctttccga cggcgcatcc gttgccgaag tcacccagga caacagcgac gaatacggca
    46381 tttcacaaat ttttattgcc attgaagtgg acaagcttat cgacggtccc acccgcgatg
    46441 ccaagctgca acgcatcatg gattacgtta ctagtgccga gcgtgctgac gaaaatcagg
    46501 ccattcgctt acccggccat gaatttacta ccctgctggc cgaaaaccgc cgtaacggca
    46561 tcactgttga tgacagcgtg tgggccaaaa tccaggcgtt atgaggagat aagtcatgat
    46621 ttttggacat atcgcgcagc ccaatccgtg ccgtttgccc gccgccattg aaaaggcgct
    46681 cgattttctg cgcgctaccg atttcaacgc cctggagccg ggcgttgtcg aaatcgacgg
    46741 caaaaatatt tatacgcaaa ttatcgattt aaccacacgc gaagcggtgg taaaccgtcc
    46801 ggaagtgcat cgtcggtata tcgatattca gtttctggcg tggggcgaag agaaaatcgg
    46861 cattgctatt gatacgggaa ataataaagt cagcgaatca ctattagagc agcgcaatat
    46921 tattttttat cacgacagtg aacatgaatc atttatagaa atgataccgg gcagctacgc
    46981 aattttcttt ccgcaggatg ttcaccgacc aggttgtatt atgcaaacag cctctgagat
    47041 acgaaaaatt gtggttaaag tcgcattaac ggcgctgaat taatcgaata atcgtctacg
    47101 ccagaacgcc tgggtaatgt tattgctcta actatattaa taattcaggt tgctgcacat
    47161 gcgacttgaa ttatgacaga catagcctca aggaatagct atgaaaaaaa tactcgaagc
    47221 aatactggcg attaatctcg ccgtactttc ctgtattgta tttataaaca ttattttaag
    47281 atatggtttt cagacaagca ttttatctgt tgatgaattg tcacgttatt tatttgtctg
    47341 gttaacgttt attggcgcga ttgtagcttt tatggataac gcccacgttc aggtcacctt
    47401 tctggtggag aaactctccc ccgcatggca gcgacgagtc gctctggtta cccattcctt
    47461 aatcttattt atttgtggcg cactggcctg gggcgcaacg ctaaaaacaa ttcaggactg
    47521 gagcgattat tcaccgatcc tcggtttacc catcggcctg atgtatgccg cttgtctgcc
    47581 caccagcctc gttatcgcat tctttgaact gcgtcattta tatcaactca tcacgcgtag
    47641 caattctcta acgtcgccac cgcaaggagc ttaatcatgg ctgtgctgat ttttctgggc
    47701 tgtctgttgg gtggtattgc tatcggtttg cctattgcct gggcactgtt gttgtgcggg
    47761 gcggcgttaa tgttctggct ggacatgttt gatgtccaga tcatggcaca aacgctggtg
    47821 aacggagccg atagcttctc cctgctggcg attccgttct ttgtgctggc gggtgaaatc
    47881 atgaatgcgg gcggcttgtc aaaacgcatt gttgacctgc cgatgaaact ggtggggcat
    47941 aaaccaggcg ggctgggcta cgtcggcgtg ctggcagcta tgattatggc gagcctttct
    48001 ggttctgccg ttgcggatac cgccgccgtt gccgctctac tggtgccgat gatgcgcagc
    48061 gccaactatc cggtcaaccg ggcggcgggg ctgattgcct ctggcggcat tatcgcgcca
    48121 attattcccc cttccattcc gtttattatc ttcggcgttt ccagcggatt atccatcagt
    48181 aagctgttta tggcgggcat tgcccctggc atgatgatgg gcgcaacgct gatgcttacc
    48241 tggtggtggc aggccagtcg ccttaattta ccccgccagc aaaaagcaac gatgcaggaa
    48301 atctggcact cttttgtctc cggtatctgg gcgctgtttc ttccagtcat cattattggc
    48361 gggtttcgct ccggtctgtt tacccctacc gaagccgggg ccgttgctgc tttctatgcg
    48421 ctgtttgtcg ccacagttat ttaccgtgaa atgaccttcg ccacactttg gcatgtactc
    48481 attggcgcgg cgaaaaccac ctcagtggtt atgtttctgg tggcctcggc acaagtttcc
    48541 gcctggctga ttaccattgc tgaactgccg atgatggttt ctgatttact gcaaccgttg
    48601 gtcgattcac cgcgtctgct gtttatcgtc attatggtgg ctatcctgat tgtcggcatg
    48661 gtcatggatt taacgccgac ggtattaatt cttaccccgg tattaatgcc tttagttaaa
    48721 gaggcaggaa tcggtccgat ttatttcggt gtcatgttta tcattaactg ctcaatcggc
    48781 ttaattacac cgcctatcgg taatgtactt aacgttattt caggggtggc aaaactcaaa
    48841 ttcgatgatg cagtcagagg cgttttccct tacgtcctgg ttttatattc attattagtc
    48901 gtatttgttt ttattcccga tctcatcatc ctccctttga aatggattaa ttaaaaggaa
    48961 aatattatga aattacactc tgtaacctac gcattattca ttgctggcct ggctgcattc
    49021 agcacatctt ctctggcggc acaatcttta cgtttcggtt atgaaacatc acaaaccgac
    49081 tcgcaacata ttgcggcgaa aaaattcaat gatttattgc aggagagaac caaaggcgag
    49141 ctgaaattaa aactgttccc ggacagcact ctcggtaacg cgcaggcgat gatcagcggc
    49201 gtacgtggcg gcaccatcga tatggaaatg tccggctcga ataactttgc cgggttatca
    49261 ccagtgatga acttgcttga tgtccctttc ctgttccgcg ataccgctca cgcgcataaa
    49321 acgctcgacg gcaaagtcgg tgatgatctg aaagcctcac ttgaaggtaa aggactgaaa
    49381 gtactggcct actgggaaaa cggctggcgc gatgtcacca actcgcgcgc accggttaaa
    49441 acccccgccg acctgaaagg gctgaaaatc cgcaccaaca atagcccgat gaatatcgcc
    49501 gcattcaaag tctttggcgc taacccgatc ccgatgccgt ttgccgaagt ctataccggg
    49561 ctggaaaccc gcactatcga cgctcaggaa cacccgatca acgtcgtctg gtcagcaaaa
    49621 tttttcgaag tgcagaagtt cctttctctg acgcaccacg cctattcccc gcttctggtg
    49681 gtgatcaaca aagcgaagtt tgatggctta agtccggagt tccagcaggc gctagtttca
    49741 tctgcacaag aagcgggtaa ctatcagcgc aaactggttg ctgaagatca gcaaaaaatc
    49801 atcgacggca tgaaagaagc gggcgtggaa gtcatcaccg atctcgaccg caaagccttt
    49861 agcgacgcac tggggaatca ggttcgcgac atgtttgtta aagatgtgcc gcagggagct
    49921 gatctgctga aagccgtgga tgaggtgcaa taaaccatga cgcaatactg gctggggtta
    49981 gattgtggcg gtagctggct gaaagccggg ctgtatgacc gcgaaggccg ggaggcaggc
    50041 gtgcagcgcc tgccgctgtg cgcattaagc ccgcagccag gctgggcaga gcgcgatatg
    50101 gcagaactgt ggcaatgctg catggctgtc attcgcgccc tgcttactca ttctggtgtt
    50161 agcggggaac aaattgtcgg tatcggcatc tccgcacagg gaaagggctt gtttttgctg
    50221 gataaaaacg acaaaccgct cgggaatgct attttgtcct cggaccgccg ggcgatggaa
    50281 atcgttcgtc gctggcagga agatggcatc ccggaaaaac tctacccgct gacccgacaa
    50341 accttgtgga ccgggcatcc ggtgtcgctg ttacgctggc tgaaagagca cgaaccagaa
    50401 cgctacgcgc aaattggctg cgtgatgatg acgcacgact acctgcgctg gtgtttaact
    50461 ggcgtcaaag gctgtgaaga gagcaatatt tccgagtcca acctctacaa catgagtctt
    50521 ggggaatatg acccgtgcct caccgactgg ctggggatcg ctgaaatcaa tcacgccctg
    50581 ccgcctgttg tcggatctgc cgaaatctgc ggggagatca ccgctcagac agccgccctg
    50641 accggtctga aagcgggtac gcccgttgtt ggcggcctgt ttgatgtggt ttccaccgca
    50701 ctctgcgccg ggatcgaaga cgaatttacc ctcaatgcgg tgatggggac ctgggcggtg
    50761 accagcggca taacccgcgg tttacgtgac ggtgaagcgc atccgtatgt ctatggtcgc
    50821 tacgttaacg atggtgaatt tatcgttcac gaagccagcc ctacctcttc cggcaacctc
    50881 gaatggttta ccgcacagtg gggagaaatc tcgtttgatg agatcaatca ggccgttgcc
    50941 agcttgccga aggctggggg cgatctcttt ttcctgccgt tcctgtacgg cagcaacgcc
    51001 ggactcgaga tgaccagtgg tttctacggg atgcaggcca ttcacacccg cgcgcacctg
    51061 ttgcaggcca tctatgaagg ggtggtgttc agccatatga cccacctcaa ccgaatgcgc
    51121 gaacgtttta ctgatgttca caccctacgc gtcactggcg gcccggcgca ctccgatgtc
    51181 tggatgcaaa tgctggcgga cgtcagcggt ctgcgtatcg agctgccgca ggtggaagaa
    51241 accggctgct ttggtgcggc ccttgccgcc cgcgtcggca ccggggttta tcacaacttc
    51301 agcgaagccc aacgtgactt gcgacacccg gtgcgcaccc tgctgccaga tatgaccgcc
    51361 catcagcttt accaaaaaaa atatcaacgt tatcagcatc tcattgccgc acttcagggc
    51421 tttcacgccc gcattaagga gcacacatta tgagccgacc acttctgcaa ctggccctcg
    51481 accactcatc acttgaagcc gcgcagcgcg acgtgacgct gttaaaagac agcgtcgata
    51541 tcgtcgaagc gggcaccatt ctctgtttaa acgaagggct tggcgcggtg aaagccttgc
    51601 gcgaacagtg cccggacaaa atcatcgttg ctgactggaa ggtcgccgac gctggtgaaa
    51661 cgctcgcgca acaggcgttt ggcgcaggcg ctaactggat gaccatcatc tgcgccgcgc
    51721 cgctcgcgac ggtagaaaaa ggccacgcaa tggcacaacg ctgcgggggt gaaattcaga
    51781 tagagctgtt cggtaactgg acgctggacg acgcccgcga ctggcatcgt attggcgtgc
    51841 ggcaggccat ttatcatcgc ggtcgtgatg cacaggccag cgggcaacag tggggcgaag
    51901 ccgatctggc acgcatgaag gcgctttcag atatcggcct tgagctttcc attactggcg
    51961 ggattacccc tgctgacctg ccgctgttta aagatatccg cgtgaaagcg tttattgccg
    52021 ggcgggcact ggcaggcgcg gcaaatccgg cgcaagtggc tggcgatttc catgcgcaaa
    52081 tcgacgctat ctggggaggt gcgcgtgcgt aatcatccgt tagggattta tgaaaaagcg
    52141 ctggcgaaag atctctcctg gccggagcgg ctggtgctgg caaaaagctg cggttttgat
    52201 tttgtcgaaa tgtcggtgga tgaaaccgac gaacggctct cacgtcttga ctggagcgcc
    52261 gcacaaagga cttcgctggt tgccgcgatg atcgaaacag gtgttgggat cccgtcgatg
    52321 tgcctgtccg cacatcgtcg ctttcccttt ggtagccgtg acgaggcagt gcgcgaacgg
    52381 gcgcgggaaa tcatgagtaa agcgattcgc ctggcgcgcg atctcggcat tcgcaccatc
    52441 cagctggcag gtgatgacgt ctattacgaa gaccacgacg aaggcacccg gcaacgtttt
    52501 gctgaagggc tggcgtgggc agtcgaacag gcggcggcat cacaagtaat gctggcggtg
    52561 gagattatgg ataccgcgtt tatgaactcc atcagcaaat ggaaaaaatg ggacgagatg
    52621 ctcgcctcac cgtggttcac cgtctacccg gacgtcggca acctcagcgc ctggggcaat
    52681 gatgttcccg ccgaactgaa actgggcatt gaccgtatcg ccgcgatcca cctgaaagac
    52741 acccagccag tcaccgggca aagtcccgga cagttccgcg acgtgccgtt tggcgaaggc
    52801 tgcgtcgatt tcgttggcat cttcaaaacg ctgcataaac tgaactatcg cggttctttc
    52861 ctgattgaga tgtggaccga aaaagccaaa gagccggtgc tggagattat tcaggcgcgg
    52921 cgttggattg aagcgcgtat gcaggaggct ggatttatat gttagagcaa ctgaaagccg
    52981 acgtgctggc ggcgaatctg gcgcttcccg ctcaccatct ggtgacgttc acctggggca
    53041 atgtcagcgc ggtagacgaa acgcggcaat ggatggtaat caaaccttcc ggcgtcgagt
    53101 acgacgtgat gaccgccgac gatatggtgg tggttgagat agccagcggt aaggtggtgg
    53161 aaggcagcaa aaaaccctct tccgatacac caacgcatct ggcgctctac cgtcgctatg
    53221 ccgaaattgg cggtattgtg catacccact cgcgccacgc caccatctgg tcacaggccg
    53281 ggctggatct ccccgcctgg ggcaccaccc acgccgatta tttttacggt gccatcccct
    53341 gcacgcgaca gatgaccgca gaggagatta acggcgaata tgaatatcag accggcgaag
    53401 tgatcattga aaccttcgaa gaacgtggca ggagtccggc acaaatcccg gcggtgctgg
    53461 tgcattctca cggcccgttc gcatggggta aaaacgccgc cgatgccgtg cataacgccg
    53521 tagtactcga agaatgcgcc tatatgggtc tattctcgcg ccagcttgcg ccgcagctcc
    53581 ctgcgatgca aaacgaactg ctggataagc actacctgcg taagcatggg gccaatgcct
    53641 attacgggca gtaatccctc acgccggggc ttcatcgccc cggcactacg aattgatatg
    53701 ttccttgctg taacgccgct tccacgctgc tggcgttaaa ccagtatgtt tctgaaaaat
    53761 ctgccggaaa tagccaacgt cattaaaccg tattgcatgg agcaggccga caacgtcgag
    53821 tcactgcata acctgttgct gcccgccgat gcgccattta cgacatttga aggcaaggga
    53881 ttgttcagcc ataagatcct gattcagacg ccaggttccc ggtatgaaca gagcaatcca
    53941 gagcaacaac ctttgtggca ttacgacgga gcaccagccg caccgacatc caccggtgaa
    54001 ttaaaaacgc cagctcaccc cggtgacaaa ggtaaaactc tcattacgat caatcatcgg
    54061 gctgtcggta atctcttccg gcagctcgct gtatcctgcg ctcgccatca atacgacgtg
    54121 ctctcctatc gggtattttg ccgtcaggct gacatagggc acccaggcat cctgcgcgga
    54181 ataacttgcc agaccgctac tgcgggactc actctctgaa atgccatagt aatagtcact
    54241 gaaattctcg tcataataga gaacgcccag cgcaggtgtc agcgacagac gacctatctg
    54301 cattttgtgg aataccgata gctcccccac ccagccgttg ctgttatcca gaacgtccgc
    54361 agctgcagag gctttcacgg ttccccaacg ctcgtggtga taccaggcaa cccccgccat
    54421 agccgtacta tcacgcttat caagctgttg catggcataa tcgtcattat cggtagggtc
    54481 aaattccagc ggcatccagg atgcggtcag gctaagttca tttttttcac tttgcgacag
    54541 aataaaacct aacgtggtct gacgaacata aaaagtatca ccttcataac taatcagcgg
    54601 aattgccttc gtattttcat tataaccgcg ataaggcgat tcattataag ccgcaccagc
    54661 accaatggat aattcagaag cagttgcgct tgccataaaa ggcaacgcaa ataacgccac
    54721 aatattgcga ttaattaaca tgtccggtat tccattttaa aataagataa aaaagagcc
//
LOCUS       DBBGLQ010000028        54065 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.028, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000028 DBBGLQ010000000
VERSION     DBBGLQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.028"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            296..592
                     /locus_tag="ACIYSB_002125"
     CDS             296..592
                     /locus_tag="ACIYSB_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429186.1"
                     /translation="MGFKGTEGKWEIMMDGDEIKIIQADSLENGAGWRSYIAICEEVQ
                     CIEDANLIAAAPDLLEALQLLLKKSKNRTTTTYPEWYGAVNKGLAAIRKALGDK"
     gene            592..780
                     /locus_tag="ACIYSB_002126"
     CDS             592..780
                     /locus_tag="ACIYSB_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429187.1"
                     /translation="MKVKITASNTSFVSVGDITEVITNHDGTQVMWSDFCKRYERVTW
                     CKLVWGVEYEELPEMHDE"
     gene            922..1290
                     /locus_tag="ACIYSB_002127"
     CDS             922..1290
                     /locus_tag="ACIYSB_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGC5429188.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            1363..1527
                     /locus_tag="ACIYSB_002128"
     CDS             1363..1527
                     /locus_tag="ACIYSB_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGC5429189.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            1496..1639
                     /gene="kil"
                     /locus_tag="ACIYSB_002129"
     CDS             1496..1639
                     /gene="kil"
                     /locus_tag="ACIYSB_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGC5429190.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            1714..2010
                     /locus_tag="ACIYSB_002130"
     CDS             1714..2010
                     /locus_tag="ACIYSB_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGC5429191.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            2016..2801
                     /gene="bet"
                     /locus_tag="ACIYSB_002131"
     CDS             2016..2801
                     /gene="bet"
                     /locus_tag="ACIYSB_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGC5429192.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2798..3478
                     /locus_tag="ACIYSB_002132"
     CDS             2798..3478
                     /locus_tag="ACIYSB_002132"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGC5429193.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVIE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3475..3657
                     /locus_tag="ACIYSB_002133"
     CDS             3475..3657
                     /locus_tag="ACIYSB_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGC5429194.1"
                     /translation="MTHPHDNIRVGTITFVYSVTKRGWVFPGISVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            3630..3821
                     /locus_tag="ACIYSB_002134"
     CDS             3630..3821
                     /locus_tag="ACIYSB_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGC5429195.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            3898..4113
                     /locus_tag="ACIYSB_002135"
     CDS             3898..4113
                     /locus_tag="ACIYSB_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGC5429196.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            4212..4433
                     /locus_tag="ACIYSB_002136"
     CDS             4212..4433
                     /locus_tag="ACIYSB_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGC5429197.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQVISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            4478..5068
                     /locus_tag="ACIYSB_002137"
     CDS             4478..5068
                     /locus_tag="ACIYSB_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGC5429198.1"
                     /translation="MHDNWGFDADLFHELVTPSIVLTLLDERERNQQYIKLRDQENEE
                     IALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRNAKLQSENAYIR
                     NRYKELDLLIGKNILVMQAAIIEWQATGDAKSGLAWIYNTLFGPGELPDESEKDAQAY
                     FNRKYAPIDEKLMALHKWFWEQSEAERAAGIRIKGE"
     gene            5070..5279
                     /locus_tag="ACIYSB_002138"
     CDS             5070..5279
                     /locus_tag="ACIYSB_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429199.1"
                     /translation="MAIAASYTMHLYCDCRQCTNGKYQTPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            5276..5425
                     /locus_tag="ACIYSB_002139"
                     /pseudo
     CDS             5276..5425
                     /locus_tag="ACIYSB_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5609..5872
                     /locus_tag="ACIYSB_002140"
                     /pseudo
     CDS             5609..5872
                     /locus_tag="ACIYSB_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5921..6145
                     /locus_tag="ACIYSB_002141"
                     /pseudo
     CDS             5921..6145
                     /locus_tag="ACIYSB_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            6138..6422
                     /locus_tag="ACIYSB_002142"
     CDS             6138..6422
                     /locus_tag="ACIYSB_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGC5429200.1"
                     /translation="MANLQLAVNGEYFDQMKSGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            6495..6662
                     /locus_tag="ACIYSB_002143"
     CDS             6495..6662
                     /locus_tag="ACIYSB_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429201.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIVHADVTNI
                     RIEELKEHKTA"
     gene            6720..6920
                     /gene="torI"
                     /locus_tag="ACIYSB_002144"
     CDS             6720..6920
                     /gene="torI"
                     /locus_tag="ACIYSB_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HGC5429202.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(7104..8039)
                     /gene="dsdC"
                     /locus_tag="ACIYSB_002145"
     CDS             complement(7104..8039)
                     /gene="dsdC"
                     /locus_tag="ACIYSB_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HGC5429203.1"
                     /translation="MEPLREIRNRLLNGWQLSKMHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHHFLMDEEILPVCSPEYAQRHALTNTVINLCHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFIIWLREQVKTTS"
     gene            8257..9594
                     /gene="dsdX"
                     /locus_tag="ACIYSB_002146"
     CDS             8257..9594
                     /gene="dsdX"
                     /locus_tag="ACIYSB_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HGC5429204.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSVD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGVTLFTILLPIALMLVKTIAELNMARESGLYILVEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            9612..10940
                     /gene="dsdA"
                     /locus_tag="ACIYSB_002147"
     CDS             9612..10940
                     /gene="dsdA"
                     /locus_tag="ACIYSB_002147"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGC5429205.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(11048..12586)
                     /gene="emrY"
                     /locus_tag="ACIYSB_002148"
     CDS             complement(11048..12586)
                     /gene="emrY"
                     /locus_tag="ACIYSB_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGC5429206.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGAVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(12586..13749)
                     /gene="emrK"
                     /locus_tag="ACIYSB_002149"
     CDS             complement(12586..13749)
                     /gene="emrK"
                     /locus_tag="ACIYSB_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGC5429207.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            14165..14779
                     /gene="evgA"
                     /locus_tag="ACIYSB_002150"
     CDS             14165..14779
                     /gene="evgA"
                     /locus_tag="ACIYSB_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGC5429208.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            14784..18377
                     /gene="evgS"
                     /locus_tag="ACIYSB_002151"
     CDS             14784..18377
                     /gene="evgS"
                     /locus_tag="ACIYSB_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGC5429209.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     IISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQINSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQVFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(18433..19578)
                     /gene="yfdE"
                     /locus_tag="ACIYSB_002152"
     CDS             complement(18433..19578)
                     /gene="yfdE"
                     /locus_tag="ACIYSB_002152"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGC5429210.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(19652..20596)
                     /gene="yfdV"
                     /locus_tag="ACIYSB_002153"
     CDS             complement(19652..20596)
                     /gene="yfdV"
                     /locus_tag="ACIYSB_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGC5429211.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(20666..22360)
                     /gene="oxc"
                     /locus_tag="ACIYSB_002154"
     CDS             complement(20666..22360)
                     /gene="oxc"
                     /locus_tag="ACIYSB_002154"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGC5429212.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(22414..23664)
                     /gene="frc"
                     /locus_tag="ACIYSB_002155"
     CDS             complement(22414..23664)
                     /gene="frc"
                     /locus_tag="ACIYSB_002155"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGC5429213.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(24177..24812)
                     /gene="yfdX"
                     /locus_tag="ACIYSB_002156"
     CDS             complement(24177..24812)
                     /gene="yfdX"
                     /locus_tag="ACIYSB_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGC5429214.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            25108..25383
                     /gene="ypdI"
                     /locus_tag="ACIYSB_002157"
     CDS             25108..25383
                     /gene="ypdI"
                     /locus_tag="ACIYSB_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HGC5429215.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGGSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEMPPPFPTKANYLIHPVR"
     gene            complement(25460..25702)
                     /gene="yfdY"
                     /locus_tag="ACIYSB_002158"
     CDS             complement(25460..25702)
                     /gene="yfdY"
                     /locus_tag="ACIYSB_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGC5429216.1"
                     /translation="MINLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            26055..26975
                     /gene="lpxP"
                     /locus_tag="ACIYSB_002159"
     CDS             26055..26975
                     /gene="lpxP"
                     /locus_tag="ACIYSB_002159"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGC5429217.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            27331..27402
                     /gene="ypdK"
                     /locus_tag="ACIYSB_002160"
     CDS             27331..27402
                     /gene="ypdK"
                     /locus_tag="ACIYSB_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGC5429218.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(27467..28705)
                     /gene="alaC"
                     /locus_tag="ACIYSB_002161"
     CDS             complement(27467..28705)
                     /gene="alaC"
                     /locus_tag="ACIYSB_002161"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGC5429219.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            29081..30778
                     /gene="ypdA"
                     /locus_tag="ACIYSB_002162"
     CDS             29081..30778
                     /gene="ypdA"
                     /locus_tag="ACIYSB_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGC5429220.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            30793..31527
                     /gene="ypdB"
                     /locus_tag="ACIYSB_002163"
     CDS             30793..31527
                     /gene="ypdB"
                     /locus_tag="ACIYSB_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGC5429221.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            31540..32397
                     /gene="ypdC"
                     /locus_tag="ACIYSB_002164"
     CDS             31540..32397
                     /gene="ypdC"
                     /locus_tag="ACIYSB_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5429222.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRSVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(32400..34895)
                     /gene="ptsP"
                     /locus_tag="ACIYSB_002165"
     CDS             complement(32400..34895)
                     /gene="ptsP"
                     /locus_tag="ACIYSB_002165"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGC5429223.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNKLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIGWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(34920..35957)
                     /gene="ypdE"
                     /locus_tag="ACIYSB_002166"
     CDS             complement(34920..35957)
                     /gene="ypdE"
                     /locus_tag="ACIYSB_002166"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGC5429224.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWVETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(35957..37042)
                     /gene="ypdF"
                     /locus_tag="ACIYSB_002167"
     CDS             complement(35957..37042)
                     /gene="ypdF"
                     /locus_tag="ACIYSB_002167"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGC5429225.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(37057..38304)
                     /gene="fryC"
                     /locus_tag="ACIYSB_002168"
     CDS             complement(37057..38304)
                     /gene="fryC"
                     /locus_tag="ACIYSB_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGC5429226.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(38326..38652)
                     /gene="fryB"
                     /locus_tag="ACIYSB_002169"
     CDS             complement(38326..38652)
                     /gene="fryB"
                     /locus_tag="ACIYSB_002169"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGC5429227.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(38871..39836)
                     /gene="glk"
                     /locus_tag="ACIYSB_002170"
     CDS             complement(38871..39836)
                     /gene="glk"
                     /locus_tag="ACIYSB_002170"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGC5429228.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            40040..41296
                     /gene="yfeO"
                     /locus_tag="ACIYSB_002171"
     CDS             40040..41296
                     /gene="yfeO"
                     /locus_tag="ACIYSB_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGC5429229.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            41411..41737
                     /gene="ypeC"
                     /locus_tag="ACIYSB_002172"
     CDS             41411..41737
                     /gene="ypeC"
                     /locus_tag="ACIYSB_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGC5429230.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(41878..43116)
                     /gene="mntH"
                     /locus_tag="ACIYSB_002173"
     CDS             complement(41878..43116)
                     /gene="mntH"
                     /locus_tag="ACIYSB_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGC5429231.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            43452..44654
                     /gene="nupC"
                     /locus_tag="ACIYSB_002174"
     CDS             43452..44654
                     /gene="nupC"
                     /locus_tag="ACIYSB_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGC5429232.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(44704..46893)
                     /gene="pdeA"
                     /locus_tag="ACIYSB_002175"
     CDS             complement(44704..46893)
                     /gene="pdeA"
                     /locus_tag="ACIYSB_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGC5429233.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFSALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(47102..47177)
                     /locus_tag="ACIYSB_002176"
     tRNA            complement(47102..47177)
                     /locus_tag="ACIYSB_002176"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47142..47144),aa:Ala,seq:ggc)
     gene            complement(47217..47292)
                     /locus_tag="ACIYSB_002177"
     tRNA            complement(47217..47292)
                     /locus_tag="ACIYSB_002177"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47257..47259),aa:Ala,seq:ggc)
     gene            47513..47872
                     /gene="yfeC"
                     /locus_tag="ACIYSB_002178"
     CDS             47513..47872
                     /gene="yfeC"
                     /locus_tag="ACIYSB_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGC5429234.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            47874..48266
                     /gene="yfeD"
                     /locus_tag="ACIYSB_002179"
     CDS             47874..48266
                     /gene="yfeD"
                     /locus_tag="ACIYSB_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGC5429235.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(48318..49733)
                     /gene="gltX"
                     /locus_tag="ACIYSB_002180"
     CDS             complement(48318..49733)
                     /gene="gltX"
                     /locus_tag="ACIYSB_002180"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGC5429236.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            49992..50067
                     /locus_tag="ACIYSB_002181"
     tRNA            49992..50067
                     /locus_tag="ACIYSB_002181"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50025..50027,aa:Val,seq:tac)
     gene            50112..50187
                     /locus_tag="ACIYSB_002182"
     tRNA            50112..50187
                     /locus_tag="ACIYSB_002182"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50145..50147,aa:Val,seq:tac)
     gene            50234..50309
                     /locus_tag="ACIYSB_002183"
     tRNA            50234..50309
                     /locus_tag="ACIYSB_002183"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50267..50269,aa:Val,seq:tac)
     gene            50314..50389
                     /locus_tag="ACIYSB_002184"
     tRNA            50314..50389
                     /locus_tag="ACIYSB_002184"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50347..50349,aa:Lys,seq:ttt)
     gene            50420..50545
                     /locus_tag="ACIYSB_002185"
     ncRNA           50420..50545
                     /ncRNA_class="other"
                     /locus_tag="ACIYSB_002185"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(50654..51538)
                     /gene="xapR"
                     /locus_tag="ACIYSB_002186"
     CDS             complement(50654..51538)
                     /gene="xapR"
                     /locus_tag="ACIYSB_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HGC5429237.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARIEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVDVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(51579..51737)
                     /locus_tag="ACIYSB_002187"
     CDS             complement(51579..51737)
                     /locus_tag="ACIYSB_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HGC5429238.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(51790..53046)
                     /gene="xapB"
                     /locus_tag="ACIYSB_002188"
     CDS             complement(51790..53046)
                     /gene="xapB"
                     /locus_tag="ACIYSB_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HGC5429239.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVTH"
     gene            complement(53106..53939)
                     /gene="xapA"
                     /locus_tag="ACIYSB_002189"
     CDS             complement(53106..53939)
                     /gene="xapA"
                     /locus_tag="ACIYSB_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HGC5429240.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
BASE COUNT        14501 a        13298 c        12675 g        13591 t
ORIGIN      
        1 gcttactaaa tatggtgttc cactatctga ggcaccacta acacctagtg gtggtaaaaa
       61 tgcacaaatg gatcttatac aattctgcga ttctggactg tgcttcgttt aagccgcata
      121 acccattaga gcagaaatat tcgctaatta aaattgaaag aataatttca tttataaacc
      181 agttccctga atcttacctg ttttgcgatg gtgaccggga aatagcagag attaagtttg
      241 aggaatgaag cagaatattc tgcctttact tttggcataa ataacagagg ctaacatggg
      301 atttaaaggt actgaaggta agtgggaaat aatgatggat ggcgatgaga ttaaaatcat
      361 ccaggcagac tcacttgaaa atggcgcagg ctggcgttcg tatattgcaa tctgtgagga
      421 agttcaatgt attgaagatg ccaatctaat agcggcagca cctgaccttc tcgaagcact
      481 tcagttatta cttaagaaat ccaaaaatag aacaacgaca acatatccag aatggtatgg
      541 agctgttaat aaaggtcttg cagcaatcag aaaagctctt ggggataagt aatgaaagta
      601 aaaataactg cttctaatac cagttttgtt agtgttggtg atattacaga agtaataaca
      661 aaccatgatg gaacacaagt tatgtggtct gatttttgta aaagatatga gcgagtaact
      721 tggtgtaaac tcgtatgggg cgtcgaatac gaagaattac ctgaaatgca tgacgaataa
      781 gcactgtgta ttcattccaa cgagtgaata cacggagcaa tgtcgctcgt aactaaacag
      841 gagccgactt gttctgatta ttggaaatct tctttgccct ccagtgtgag ggcgattttt
      901 tatctatgag gatatgaata gatgtcaaac atcaaaaaat acatcattga ttacgactgg
      961 aaagcatcaa tagaaattga aatcgaccat gacgtaatga cagaagaaaa acttcaccag
     1021 attaataatt tctggtcaga ctctgaatac cgactcaata aacacggctc tgtattaaat
     1081 gctgtattaa tcatgctggc gcaacatgct ctgcttatag caatttcaag cgacttaaat
     1141 gcatatggtg ttgtgtgtga gttcgactgg aatgatggaa atggtcagga aggatggcct
     1201 ccaatggatg gtagcgaagg aataagaatt accgatatcg atacatcagg aatatttgat
     1261 tcagatgata tgactatcaa agccgcctga gcgcggcgtt accgcatacc aataacgctt
     1321 cactcgaggc gtttttcgtt atgtataaat aaggagcaca ccatgcaata tgccattgca
     1381 gggtggcctg ttgctggctg cccttccgaa tctttacttg aacgaatcac ccgtaaatta
     1441 cgtgacggat ggaaacgcct tatcgacata cttaatcagc caggagtccc aaaaaatgga
     1501 tcaaacactt atggctatcc agactaaatt cactatcgcc acttttattg gcgatgaaaa
     1561 gatgtttcgt gaggccgtcg acgcttataa aaaatggata ttaatactga aactgagatc
     1621 aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc atttcgcggg
     1681 cgatattttc acagctattt caggagttca gccatgaacg cttattacat tcaggatcgt
     1741 cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga gaaagaggca
     1801 gaactggcag acgacatgga aaaaggcctg ccccagcatt tgtttgaatc gctctgcatc
     1861 gatcatttac aacgctgcgg ggccagcaaa aaagccatta cccgtgcatt tgatgacgat
     1921 gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac cattgctcac
     1981 catcaggttg atattgattc agaggtataa aacggatgag tacagcactc gcaacgctgg
     2041 ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa ctgatcacca
     2101 ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc gcattgttga
     2161 tcgtcgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc ttccctgaca
     2221 agcagaacgg catcgttccg gtggtgggcg ttgatggctg gtcccgtatc atcaatgaaa
     2281 accagcagtt tgatggcatg gactttgagc aggacaatga atcatgtaca tgccggattt
     2341 accgcaagga ccgtaatcat ccgatctgcg ttaccgagtg gatggatgaa tgccgccgcg
     2401 aaccattcaa aacccgcgaa ggcagagaaa tcaccggacc gtggcagtcg catcccaaac
     2461 ggatgttacg gcataaagcc atgattcagt gtgcccgtct ggccttcgga tttgctggta
     2521 tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact gcagaacgtc
     2581 agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt aacactctgc
     2641 tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc cagatatttc
     2701 gccgcgacat tcgcgcatcg tcagaactga cacaggccga agcagtgaaa gctcttggat
     2761 tcctgaaaca gaaagccact gagcagaagg tggcagcatg acaccggaca ttatactgca
     2821 gcgtaccggg atcgacgtaa gagctgtcga acagggggat gatgcatggc acaaattacg
     2881 gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaagcccc gctcaggaaa
     2941 gaagtggcct gacatgaaaa tgtcctactt ccacaccctg ctggctgagg tttgcaccgg
     3001 tgtggctccg gaagttaatg ctaaggcgct ggcatgggga aaacagtacg agaacgacgc
     3061 cagaaccctg tttgagttca cttccggcgt gaatgttatt gaatccccga tcatctatcg
     3121 cgacgaaagt atgcgtaccg cctgttctcc cgatggttta tgcagtgacg gcaatggcct
     3181 tgagctgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg gtggtttcga
     3241 ggccataaag tcggcttaca tggcccaggt gcagtacagc atgtgggtga cacgaaaaga
     3301 tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggactgc attatgtcgt
     3361 ggttgagcgg gatgaaaagt acatggcgag ttttgacgag atggtgccgg agttcatcga
     3421 aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat ggcgatgacg
     3481 catcctcacg ataatatccg cgtaggcacg atcactttcg tctactccgt tacgaagcga
     3541 ggctgggtat ttcccggcat ttctgttatc cgaaatcccc tgaaagcaca gcggctggct
     3601 gaggagataa ataataaacg gggagctgta tgcacaaagc atctcccgtt gagttaagaa
     3661 cgagtatcga gatggcacat agcctcgctc aaattggagt caggtttgtg ccaataccag
     3721 tagaaacaga cgaagaattt catacgttag ccgcatccct ttcacaaaag ctggaaatga
     3781 tggtggcgaa agcagaagca gatgagagag accaggtatg acaaccacgg aatgcatttt
     3841 tctggcagcg ggcttcatat tctgtgtgct tatgcttgcc gacatgggac ttgttcaatg
     3901 acacctcagc aggaaaacgc ccttcgcagc attgcccgtc aggctaattc tgaaatcaaa
     3961 aaagccagac agcagtttcc ggataaaaac gtcgatgaca tttgccgtag cgtactgaag
     4021 aagcaccgcg aaacggtaac gctgatggga ttcacaccaa ctcatttaag cctggcgatc
     4081 ggcatgttaa acggcgtttt taaggaacgg tgaacatgaa aagcaaaatt atcagggagc
     4141 tacaggctcc ttttttatta ttcgcattca ccctcaagcg tattaaccaa caattcaggg
     4201 attaatggaa gatggcagac atcattgatt cagcatcaga aatcgaagaa ttacagcgca
     4261 atacagcaat aaaaatgcgt cgtctgaacc accaggttat atctgccact cattgttgtg
     4321 agtgtggcga tcccatagat gagcgaagac gcctggccgt tcagggttgt cggacttgtg
     4381 caagttgcca ggaagatctg gagcttatca gtaaacagag aggttcgaag tgagcgtaat
     4441 tcactctcag gcactgcgtg aagcggcaga gcaggcaatg catgacaact ggggatttga
     4501 cgcggacctt ttccatgagc tggtaacacc atcgattgtg ctgacactgc tggatgaacg
     4561 ggaaaggaac cagcaataca tcaaactccg cgatcaggag aacgaggaaa ttgcgctaac
     4621 ggtagggaag ctgcgtgttg agctggaagc cgcagagaat aacctcattg atagtgaatg
     4681 ccatgttgct gaactggaag aagcgctacg cgataagcag gcgttacttg aagcctcaga
     4741 aaagcgcaac gcaaaattac aaagcgagaa tgcatacatc cgcaaccggt acaaagaact
     4801 ggacctatta atcgggaaaa acattctggt catgcaggct gccattatcg aatggcaggc
     4861 aactggcgac gctaagagcg gactagcatg gatttataac acactgtttg gccctggcga
     4921 attaccggac gaatctgaga aagatgctca ggcctacttt aatcgcaaat atgcaccgat
     4981 tgacgaaaag cttatggcgc ttcacaagtg gttttgggaa caaagtgaag ccgagcgcgc
     5041 tgctggtatt cgcatcaaag gagagtgata tggctattgc cgcaagttac accatgcatc
     5101 tctactgtga ttgtcgccag tgtacaaatg gtaaatatca aacgccagac ttcggtgaat
     5161 atatcggtac gtcatgggct ggctgtgcaa aagaggcgcg caaggatggc tggcgaataa
     5221 gcaaagacaa aacgcgtgct tttgcgcccg ggcataaagt tttgaggatt aacaaatgac
     5281 cactattacc aaagaacgtg cggagattaa atcattcatc acaggcttcc tgagcgaccc
     5341 ggcgcacgat aaccaatctt cagacagcct gcttgccaat gtgtttcgta tcgcgctggc
     5401 atcgctggaa gcagaaccgg tggcgtggat gcgtgatgac gcagatggtc gtgagtataa
     5461 cgctcgcaat gaattttctg gtggaggggg aggaattcca ctctacgcca cccctcaggt
     5521 gcaggtagtg ccggaagaaa tgtattggca ggatgcgcca gttgaaggca gcagcaaagc
     5581 ggctgcatac gctacaggct ggaacgattg ccgcgaagcc atgcttcagt ccggaaactt
     5641 tcgggaaaac aagaattcgt caaccaataa ttttcgggaa atcgcggaaa cgtcaaccaa
     5701 ctatccggta attcctagtg aggtgttgtc tgcaatccag aaggttgcca agattcgtgc
     5761 cgatttcgat gattttgacg gtgacaggcg aggtatcggt gattgtctgg atgaggctga
     5821 gcaagagctt atcgttacca ttaacaaata tgccagtcag ttggcagcag aacctatagc
     5881 gcctaatgac gttcgagagc agacagccat tccgcaagtt ccggtaactc cggatagttg
     5941 gataagctgt agtgagcgaa tgcctgatga tggtcagcac gtaattattt tatgtgatgg
     6001 cgcattcgtt ctttatgcgc aatatcgaga cggtgaattt tttgatgtcg tccgcaatgg
     6061 tgaggagttc ttcgaaacgc agagccgcaa tgtaacccac tggatgccgc taccagaacc
     6121 gccgaaggag gttaaccgtg gctaaccttc aactggcagt taacggtgaa tatttcgacc
     6181 agatgaagtc aggcgagaaa acggaagagt atcgcctgtg taatgactac tggaataagc
     6241 gaattatgtt ccgggagtat gaccgcctga ttatcacaaa gggatatccg aagcgcgacg
     6301 attccagtcg cagaattgat gttccgtatg acggatatga aatcaagaca atcacacatc
     6361 cgcacttcgg cgataaaccg gtaaaggtgt tcgcgataaa ggtgaatatc ggcaatgaat
     6421 aacaatcttc gcactcgcgg ggatttcttt tatctgaact cgctacggcg ggttttgttt
     6481 tatggagatg ataaatgcac ttccgagtca caggtgaatg gaatggagag ccattcgaca
     6541 gggttatcga agcagaggac atcaacgact gctataacca ctggatgata tgggcgcaga
     6601 tagtgcatgc agacgtaacc aatattcgaa ttgaagaact gaaagaacac aaaaccgcct
     6661 gatggcggtt ttttcttgcc tgatttgcag gttcgattcc ctattcggag atagcactca
     6721 tgcaacacga actacagcct gattccctgg ttgatttgaa attcatcatg gccgatactg
     6781 gcttcggtaa aaccttcatc tatgaccgga ttaagtccgg ggacctgcct aaagccaaag
     6841 ttatccacgg gcgagcaaga tggttatatc gtgaccattg tgaattcaaa aataagctct
     6901 taagccgcgc caatgggtaa aatagcgggt aaaatttttt tcacatctaa aaaacaccat
     6961 tccaatcaag cccctgccgc ttcaagtaga tgtctgcagg ggacaccatc cttgtgttta
     7021 tccctaaaac cacataaaaa ccgtaaatta aattcgaact agcaggccga ataaggcaca
     7081 ccaggaactc gtggttttat tcattatgaa gtggttttta cctgttctct tagccagata
     7141 ataaacgctt caatttttgg ccactgcctg cccggtaatg tggtgatgta ataatgctga
     7201 tggcatttca ccgtcatatc gccaaacggc gcgacgagct caccactggc aagccttttt
     7261 tgtaccaggc gttttcttcc catcgccacc ccaatatgat tcatcgcggc aataacagct
     7321 aaatcagaac gatcaaagcc aattccagaa gatgtcggca aattaaccgc ataatgttgc
     7381 gcccaactat gccattcatc cgtaccggag tcgttgctcc atgcctgtct gtcatggagc
     7441 aacgtacagt gacacaggtt aattaccgtg ttggttaaag catgtctttg agcgtattcc
     7501 gggctacata ctggcaggat ttcttcatcc atcagaaagt gatgagtgag ttgcgctgac
     7561 ggcgcatcat caaagtagat cgccaaatcg attccggcac gttgcagatt gacgttgtca
     7621 ttaccagtga gcacggtgag cgaaatagac ggataccggc gtgtaaagtc acctaatgcg
     7681 ggcaccaacc agcattgggc gatagagggc cgggaataca gagttaacgt tcccgataac
     7741 tcctgatttt tgatatcaag aatttcctgg ttcagggtat ccagcgacga ttttagcgcc
     7801 caataaacac gtttcccctc gtgcgttaat tccactttgc gatgggaacg aacaaacaac
     7861 tgaatgccca attcttcttc cagctgattg atacggtgac ttaccgcact ggggctcagc
     7921 gacaattcct ctgccgccag ggcgaaggac tgatgcctgg cagccacttc aaaagtatgc
     7981 atttttgata attgccagcc gttaagcagc cgatttctta tttcacgaag gggttccata
     8041 atcacctcat ttttcgctta agtgtaaaaa aatagcggca aaatttcagc tatgagatga
     8101 gctaaagtga accatatctc aattcacctt catttttaga tgtaaatcac tccattgatg
     8161 caatttacct catgtgaaag gcaaatttta tcgtttgtca gcctgcgttg tttttttgtc
     8221 caatatcatc aggttaatca caggggaagg tgagatatgc actctcaaat ctgggttgtg
     8281 agcacgctgc ttatcagcat cgtgttaatt gtactgacca tcgtgaagtt caaattccac
     8341 ccgtttctgg cgctgttgct ggccagcttc ttcgtgggaa cgatgatggg catggggcca
     8401 ctggatatgg taaatgctat tgaaagtgga attggcggaa cgctggggtt cctcgcagcg
     8461 gttatcggcc ttggcacgat actgggaaaa atgatggaag tatccggggc cgcagaaaga
     8521 attggtctga cacttcaacg ctgccgctgg ctttcagttg atgtcattat ggtgctggtt
     8581 ggcctgattt gtggcatcac gctgtttgtt gaagtgggcg tcgtgctatt gattcctctg
     8641 gctttttcaa ttgccaaaaa aaccaatacc tcattattaa agcttgccat tccgctatgt
     8701 accgcattga tggcagtgca ctgcgtggtt cctccacatc cggctgcttt atatgttgcc
     8761 aataagctgg gcgcagatat cggttcggtg atcgtctacg gtttgctggt tgggctgatg
     8821 gcatcactga tcggtggccc acttttcctt aaatttctgg gtcaacgact gccctttaaa
     8881 cctgtaccca cagagtttgc agatctcaaa gttcgcgatg aaaaaacact accgtcatta
     8941 ggcgtaacgt tattcaccat actgctaccc attgcgctga tgttggttaa aacgattgcc
     9001 gaattgaata tggcgcgtga gagtggtttg tatatcttgg ttgagtttat tggcaaccct
     9061 atcactgcca tgtttatcgc cgtgtttgtc gcctattatg tgttgggtat acgccagcat
     9121 atgagcatgg ggacgatgct cacacatacg gaaaatggct tcggttctat tgctaatatt
     9181 ttgctgatta tcggggccgg aggcgcattc aacgccattt taaaaagcag cagtctcgct
     9241 gatacgctgg cagttattct ctccaatatg catatgcacc cgattcttct ggcctggtta
     9301 gtggctctta ttctgcatgc ggcagtgggc tccgctaccg tggcaatgat gggggcaacg
     9361 gcaattgttg cacccatgct gccgctgtat cccgacatca gcccggaaat tattgcgatt
     9421 gctatcggtt caggtgcaat tggctgcact atcgttacgg actcgctttt ctggctagtg
     9481 aagcaatatt gcggcgctac gctcaatgaa acatttaaat actatacgac agcgacattt
     9541 atcgcttcag tcgtcgctct ggcgggcaca ttcctgctgt catttatcat ctaagcgcaa
     9601 agagacgtac tatggaaaac gctaaaatga actcgctcat cgcccagtat ccgttggtaa
     9661 aggatctggt tgctcttaaa gaaaccacct ggtttaatcc tggcacgacc tcattggctg
     9721 aaggtttacc ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat
     9781 cccgttttgc accctatctg gcaaaagcat ttcctgaaac tgctgccact ggggggatta
     9841 ttgaatcaga actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc
     9901 aaccgatcag cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccggctcca
     9961 taaaagcacg cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag
    10021 cggggttgct gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt
    10081 tctttagcca atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca
    10141 ttatgagcgc ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat
    10201 ggaaaaaagc gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg
    10261 gtgttgccgt cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg
    10321 atgacgaaaa ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag
    10381 cgcaatttgc ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc
    10441 cgtgtggtgt tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg
    10501 atcatgttca ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc
    10561 atacaggatt acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag
    10621 cggatggcct tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gagcgtctgc
    10681 tggatggctt ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc
    10741 aggaagaagg tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg
    10801 tgtgtgcatc agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata
    10861 ccactcatct ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat
    10921 atctggcaaa aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga
    10981 gcgatgctgc cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct
    11041 tttatcatca cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg
    11101 taagtaagat aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg
    11161 actgctgggt tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt
    11221 tatccataat ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca
    11281 actgactatg gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa
    11341 ctgctcctga caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat
    11401 ctggcaagcc tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc
    11461 cctgaaaaaa ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag
    11521 aacgccagta atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt
    11581 ctattttgtt gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg
    11641 gcgcataagc aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg
    11701 gcataaggac gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa
    11761 tggtgaagtt acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact
    11821 cccaaatgac taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac
    11881 tcgagttgaa ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac
    11941 cgagcactaa cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc
    12001 ttcctttaag taaggttaag cataatgtca ggacgataat ccccataggg acattgatta
    12061 aaaatatcca accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata
    12121 tcggagcgat aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg
    12181 gataattcct taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct
    12241 gaacgactct aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag
    12301 acagcgaaaa aaaagtgact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca
    12361 acctgccagt aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg
    12421 tgccttcgtc tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa
    12481 tagtggaatc caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata
    12541 acgtcccacc ggttaatggt gccggagttg atttagtgat tgccattaaa gttgtccatt
    12601 atgcgaaata atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt
    12661 actggtataa gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc
    12721 ttcgttcttc gtatcaatag ttgctgtcat cgataaacca atacgcaagg ggtgttccat
    12781 gagttctttt ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt
    12841 ccctgtcgca ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc
    12901 tgtcacccga ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga
    12961 ttgaccaatc cgtacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg
    13021 acgtgccggt acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac
    13081 acttctctgg gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa
    13141 ccaggcttct ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt
    13201 gttcattact aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt
    13261 gctacttatt aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt
    13321 cgctaacggc actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga
    13381 agcgacttcg gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat
    13441 atttgccaga ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga
    13501 aactaaaatg tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact
    13561 acctgagact tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact
    13621 aatcatgtct tctaattcca ttgaccaata ggcataggca cctgaaaacg caataaataa
    13681 aactaccgcc aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat
    13741 ctgttccact attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc
    13801 attcattctt catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg
    13861 cctaatttaa atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac
    13921 aattcttacg cctgtaggat tagtaagaag acttatagtg ccaacttgaa actataaatc
    13981 atcggtacaa tccctgattt tattgttgac atttcattta tgccgactat ttatatggta
    14041 tacttgtcga attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga
    14101 tgacgcctta tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata
    14161 atctatgaac gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt
    14221 attgatcaaa aacgatattg aaatcttagc agagttgact gaaggcggaa gtgccgttca
    14281 gcgggtggaa acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa
    14341 cggtatccag gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattatcgt
    14401 ctccgctaaa aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggttt
    14461 cgtgagtaaa aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg
    14521 ctactgctat ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca
    14581 aaaactcgac tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa
    14641 ggataataat gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa
    14701 aagtcgcctg atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcaca
    14761 acgtaacaaa atcggctaac cacatgaagt ttttacccta tatttttctt ctctgttgtg
    14821 gtctttggtc gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta
    14881 gtaacaaccg tgtcacactt gatccactac gtctgagcaa caaggaatta cgttggttag
    14941 cgagcaaaaa aaatcttgtg attgcagtac ataagtccca aacggctacg ttgttgcata
    15001 ccgattcgca gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag
    15061 cgttaaatat caaattaaca ctccgggaat acgcagatca tcaaaaagca atggacgcgc
    15121 ttgcagaagg tgaagtcgat atagtgttat cacatttagt tacttcgccg cctcttaata
    15181 atgacattgc tgcaaccaaa ccattgataa ttacctttcc ggcgctggta accacccttc
    15241 acgactcaat gcgaccgctt acctcaccaa aaccagtaaa tattgctcgg gtagcaaatt
    15301 accccccaga cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa
    15361 atttatatca ggcattagca tccgtctcag ctgggcacaa tgattacttt attggtagta
    15421 acatcattac cagcagtata atttcccgct atttcactca ctccttaaat gtagtgaaat
    15481 attataactc gccgcgtcaa tataattttt tcttgaccag aaaagaatct gtcattctta
    15541 atgaagtact caatagattt gttgatgctt taacaaatga agttcgctat gaagtatcac
    15601 aaaattggct tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac
    15661 atgaaaaaca gtggattaag cagcatccca atttaaaggt gctggaaaat ccttactcgc
    15721 ccccctattc tatgacggat gaaaatggct cggttcgggg cgttatgggg gacattctta
    15781 atattattac cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc
    15841 atgctggaac acagcttagc cccggaggat gggatataat acctggcgct atttatagtg
    15901 aagatcgaga aaataatgtt ttatttgctg aagccttcat aacaacgcct tacgtttttg
    15961 tcatgcaaaa agcgcctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc
    16021 catattatta tgagctgcat tcgcaattaa aagagatgta tccggaggtt gaatggatac
    16081 aggtcgataa tgccagcgct gcatttcaca aggttaagga aggtgaactt gatgctctgg
    16141 tcgcgacaca gataaattcg cgttacatga tcgatcatta ctatcctaat gaactttatc
    16201 attttcttat tcctggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac
    16261 cggaacttaa ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc
    16321 gcctgacgga aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctatata
    16381 gcgagcaatt ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat
    16441 ggggattcta cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa
    16501 accaaatatc attccgaaaa gcactctcgg attccttacc gaatccaact tatgttgtaa
    16561 actggcaagg taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt
    16621 actacaaaaa tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt
    16681 ctaatgcgca tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat
    16741 ttgaaattga taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc
    16801 ttcctgcaag tgacaatgca gtatatattt gtggttggca agatattact gaaacgcgtg
    16861 atctaattaa tgcactcgag gtagaaaaaa ataaagcgat aaaggctacc gtagcaaaaa
    16921 gtcagtttct ggcaacgatg agtcacgaaa taagaacacc aataagctct attatgggct
    16981 tcctggaact tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac
    17041 ttgcctacgc caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca
    17101 aaattgaatc gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc
    17161 agaacacttg tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca
    17221 gcagtacgtt tcctgaacat tacctggtta agatcgaccc tcaggtgttt aagcaggtct
    17281 tatcaaattt actgagtaat gctctcaaat ttaccaccga gggggcagta aaaattacga
    17341 cctccctggg tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg
    17401 gaagtggatt atcgcaggaa gaacaacaac aactgtttaa acgctacagc caaacaagtg
    17461 caggtcgtca gcaaacaggt tctggtttag gcttaatgat ctgcaaagaa ttaattaaaa
    17521 atatgcaggg cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca
    17581 caatcccggt agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacaaccca
    17641 tcacactacc tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat
    17701 tactcaaacg ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc
    17761 aagcgctaca caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc
    17821 cgaatatgga tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca
    17881 tctgggggct tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca
    17941 tgaacttatg tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt
    18001 tacaccaagt tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa
    18061 ataatacggc gaacgatcta caactgatgc aggagattct catgactttc cagcatgaaa
    18121 cgcataaaga tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc
    18181 atcagtgtat tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta
    18241 atattagcca tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc
    18301 agttgctgaa ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct
    18361 gtcagaaaaa tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag
    18421 cacatcttac atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt
    18481 tgccgctccc ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg
    18541 catcattatt cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat
    18601 ggcctcagcc acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa
    18661 ccaaacttca gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg
    18721 gttttgtacg cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc
    18781 cagtgcctgg cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt
    18841 atcctgagta ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg
    18901 tggtgacttc ccagttgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc
    18961 atcaaacatc gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc
    19021 actcactatt ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac
    19081 gcgcactggc ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat
    19141 gatggtatcg taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga
    19201 agcatatatg aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc
    19261 cattgtacct gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa
    19321 tatactttta tcgtgatcat tctttaaatc aagaaccaca ctctctttgc catgattaat
    19381 aaaactgtaa tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc
    19441 atgacccggc ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc
    19501 gaaaggtcca ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt
    19561 gctttcatta tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc
    19621 ggcatttatg taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc
    19681 acaagggagc cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac
    19741 gggtgtagac attaaaccgg ctggcaataa tgattccgga gaatgccggc ggtaatgcgc
    19801 ctgccagtac catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa
    19861 gcagtgccag tggcatcaga atcagcttca ggaaggtgtt ataagcaatt tcggcactga
    19921 actcgaattt atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag
    19981 ccttcgcaat cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca
    20041 ccaggatcgt tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg
    20101 cgctcagatt actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac
    20161 caataggaat agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg
    20221 atacggaatc accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg
    20281 aacctgcaat taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc
    20341 aaccgaacca ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac
    20401 gagtgtccgc aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag
    20461 gaagcgcgta gtttaatacc agtttattaa atgcccgagc ttgatcttct gaaaatgttt
    20521 ctcgtctgcc gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa
    20581 taaaaaatgt tagcataata gtatttccga taagggtccc ctcaccatcg cataatgagt
    20641 tggcaagagg agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg
    20701 taatatggcc actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt
    20761 tgcgcgactg aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat
    20821 agccaacgcc acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg
    20881 ttggtgatgg tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat
    20941 tattaaaaat aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc
    21001 cactgaaacc aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc
    21061 tagcaccgat ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac
    21121 gacgacgtgg tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt
    21181 cattaactaa ataaatatcc tggttctcgc gcaatacgtc gcgcacagca cttaatgcat
    21241 taaagtaatt taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt
    21301 gctgcttgtg gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt
    21361 tcagttctgc cagcatacct tgcatactgg atgcaatatc accaacgact ggcacagcaa
    21421 tggggcggtt gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg
    21481 ccgcccatcc ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga
    21541 caacgtcagc atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat
    21601 cttcaaggat ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa
    21661 attcacgaag ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg
    21721 gccgttcagc ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata
    21781 atgctggcga cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg
    21841 tcgcggccag gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag
    21901 agacccggat agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg
    21961 ctgctttggc atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct
    22021 gtaggtcgac gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta
    22081 ccgttgcgtt ggccaatgcg gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc
    22141 agatccccgg tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct
    22201 catgacgaaa accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca
    22261 caggaatacc tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga
    22321 tgatatgcat accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt
    22381 ttataaattg ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc
    22441 agcaatttca tcgtcgctat aacccagctc ctgcaataca gcagcggtat gttcacctaa
    22501 tagcggcgca gctttaatat ccggcgtaaa ggcagagaat ttcattggac aaccaacggt
    22561 cagatatttt ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga
    22621 gggatcaagt gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt
    22681 caaataggcc accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa
    22741 aatatcgaaa atatgtggct gtcgtgcatg ggctgtactg tatgccggat cggtaatcca
    22801 ttctggtttg ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata
    22861 aatataggcg ttaggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc
    22921 gccaccacct gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg
    22981 cgggtattct tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg
    23041 gcaaaggttc aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt
    23101 ttcgcgatgc agcaaagcag caagtaaacc gatcagcaaa tgcattccgg tgttgctgtc
    23161 acccaacgct gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc
    23221 gccacccgct gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc
    23281 atcaaaccct ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca
    23341 ggtgaagccc atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc
    23401 gcggatcagc ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga
    23461 acgtttgtta ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag
    23521 ctggtgacgc gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa
    23581 ccaggccagc atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt
    23641 aattccttga agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt
    23701 tagcgtttat ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg
    23761 cactcgatat ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa
    23821 ttcctttctg catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt
    23881 ggtaatcaga gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg
    23941 tgatttacaa gcaagggtat gattacgctt tttaaagcgt accataaaac ataacataat
    24001 aaatgatttg tgatgacacg ggattattgc ggtttacttt gaggaatata aaatgcgagt
    24061 ttatagcacc gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa
    24121 aaaattgtcg gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag
    24181 ttcacaaaca gtgcttcgct atcgacgatg attccatctt ccgcaccttt aagcgccagg
    24241 tttgcctcgt agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga
    24301 gtctgtttaa gtggcatcag atattggttt tccattacgc caacaccggc caggcgaagc
    24361 tcttccattg cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg
    24421 gcttcttttt cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca
    24481 atatattggt catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg
    24541 gtactgtcat ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga
    24601 tcgccatgaa acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt
    24661 tcagagatac gctctgctgc cgctgccgtt ttctgggttt gctgaacttg ctgagtttgc
    24721 tgttgtgccg ctaccggagc attatcagca gcccatacag ttgaagatgc cagaattgct
    24781 gtgaccatcg tggccataat taaacgtttc atacttacct cattctttaa cagttttaga
    24841 aaacgccgcc atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt
    24901 gaaagggtta tcttacagtt gtagttatta tcgcaagaag tattttagtc ttacaattgt
    24961 aggaatatgc ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat
    25021 tatatcgatg taaatactga ttaattaaat gtaaatacag ccataacatc gtaggttctc
    25081 agtgaaccat tttcaggata actttctatg aaagtaaact taatactttt cagcttattt
    25141 ttattggtct ctattatggc atgcaatgtt tttgcatttt ccatttcggg tggtggaagt
    25201 gagaggagct ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa
    25261 cttcagccat cacaggcgat tttgtttaag atgagagaag atgcgccacc attaaacctc
    25321 acagaagaaa tgccgccccc ttttccgaca aaggcgaatt atcttattca tcctgtgcga
    25381 tagcgtcttt cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca
    25441 aatgagccat cctgacccac taccgcctgc cataccccac cataaagcga atcatcaagc
    25501 cacaagccgg ggcaagaaac ataaatatcc ccgtcactag ttcattaccg ccagttaacc
    25561 acatggtgaa gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg
    25621 ccgataccac gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga
    25681 gaaacatcca taaattaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc
    25741 agtcacttta atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa
    25801 aacagcatgt taaatgcaag actgttgtgt acggaaaaat atttactttg cacgattaat
    25861 aatcagtagc tgaaagcagt cagcgagtga taaagtacag ataccgcatt catgctcctc
    25921 gtggttatgt cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc
    25981 ccaaccaatt gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc
    26041 tacagaaggt aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct
    26101 actggctcac atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg
    26161 ttctctgctt tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg
    26221 aatctatcgc ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg
    26281 agaagatgat tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg
    26341 catggttctg gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc
    26401 ttaaacgcgc acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc
    26461 tggaactggg cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac
    26521 ataataatca gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga
    26581 tgatcggcag aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat
    26641 ggtttgctcc cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg
    26701 tggaaaatgt cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca
    26761 tgttgaccgt aacgatggta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc
    26821 cagagatgga aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta
    26881 tcgagaaaga gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa
    26941 cgcgcccggt gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc
    27001 tcaaatcggc cataataacc tcatggtttt tatggctttt ttcttaaggt tttttccttc
    27061 gctgaatatc taattattac cgtcagaaat taaactgtcg ccgatatgag acactcgtaa
    27121 gttactaaaa gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag
    27181 cgccgtctcg agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc
    27241 ttttaaggtc gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg
    27301 cgtaacagta attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt
    27361 atggtcatcg tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga
    27421 aaaacaggag ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt
    27481 cgtgaatatg tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc
    27541 ctctgatggc ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat
    27601 caccatagtc gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct
    27661 tcttggcaaa ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat
    27721 acatcgaagc cttcggcatt tcaaccatcc agcccgcttc atgcagccct ttaaccaata
    27781 catcacggcg gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcca
    27841 gcgccgcaat cgccgccacc tgcaacggcg taaaggtgcc gtaatcgtga tagcttttaa
    27901 tacgagccag ggcgctgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg
    27961 ccatgttgta gcttttcgac agcgtaaaga actcgactgc cacatcgcgt gcacccggta
    28021 cctgcataat tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat
    28081 ggaccaccag cacatcgtag cgtttcgcca gcgccactac tttttcgaag aactccagtt
    28141 ccacgcattg cgcggttggg tttgacggga agccgaggat catcatcttc ggtttcggat
    28201 aactttcacg aatggctcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca
    28261 ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat
    28321 tcggcaccag caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct
    28381 ctttcgaacc aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat
    28441 cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac
    28501 catgcgtgtc cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg
    28561 caccgtccgg gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca
    28621 ttttcagttc agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc
    28681 gacgttcagg gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc
    28741 cgagcgacgc tgccacgatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc
    28801 cagcgtttta gaacatttta ttaatgacgt taaatgagga actctgcttt aaaaacagga
    28861 aatttatctg cgctaacaag aaaattacca ctcattttat taacataata atttcaataa
    28921 atttaccaac cagaagtgca ttatcaagag atgcattgct aatacaccat ttatctatca
    28981 atcccctttt aaaattgtgg tttttcctca aaaacggcct gctggtcatc agccttcagg
    29041 ttacctatca tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat
    29101 gctgctggcg gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg
    29161 tatccgcctg tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc
    29221 cgttaccgcc attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga
    29281 aggctcgctg gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc
    29341 gtgggtaggc atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg
    29401 cggcgtgacg gcgatcccct gctttatcac cagcattctg gcgggttgta tatcgggctg
    29461 gatcaacctg aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt
    29521 gtgtgagacg ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat
    29581 cgatatcgtc tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat
    29641 tgtgcttctg gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa
    29701 gctggcgctg gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc
    29761 attacgcaag gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac
    29821 taataccgat catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga
    29881 tgacttcatt agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa
    29941 caatgatgaa gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga
    30001 gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc
    30061 gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc
    30121 acgcgccgag cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa
    30181 aattaatccc cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa
    30241 tccggatacc gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga
    30301 attaaaagac gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta aagactatat
    30361 tgccattgag caggcccgct ttggtgacaa gctgacggtt atctatgata ttgatgaaga
    30421 ggtgaattgc tgcattccca gcctgctgat ccagccgttg gtggagaacg ccattgtcca
    30481 cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa
    30541 tcgggtacgc attgcggtgc gagataccgg gcacggcatc gatccaaagg tgattgagcg
    30601 ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt
    30661 gaagttattg tatggcgagg ggctgcatat ccgccgcctg gagccgggga cggaaattgc
    30721 gttttacatt cctaaccaac gcaccccagt cgcctcacag gctacgttat tgctttgagc
    30781 cggagtgata ttgtgaaagt catcattgtt gaagacgaat tcctggcaca acaggaactg
    30841 agctggctaa ttaaagagca cagccagatg gagattgtcg gcacctttga cgacggtctg
    30901 gacgtgttga agtttttgca gcataaccgc gtcgacgcca tttttctgga tatcaatatt
    30961 ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc agttcgccca taaaccgttt
    31021 attgtgttca tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact ggaggcgttt
    31081 gactacattc tcaaaccgta tcaggagtca cgtattaccg ggatgctgca aaaactggaa
    31141 gcggcctggc aacaacagca gaccagcagt acgcctgccg cgacggtaac gcgtgagaat
    31201 gacaccatta atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat
    31261 tacgccgaag cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg
    31321 ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat
    31381 cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc
    31441 tacattctgc gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa
    31501 gaatttcgcc agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc
    31561 gccgatcaac aatttttcgc cgatctgttc agcggcctgg tgcttaaccc gcaactactg
    31621 gggcgcgtct ggtttgccag ccagcctgcc tcgttgccgg tgggcagttt atgtattgat
    31681 tttccccgtc tggatatcgt gctgcgcggc gaatacggca atctgctgga agcaaagcag
    31741 caacgtttgg tggaaggaga aatgctgttt attccggcgc gcgcggctaa tttaccggtc
    31801 aacaacaaac cggtgatgct gttaagcctg gtgttcgctc cgacctggct tgggttatcg
    31861 ttttacgata gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc
    31921 ctgcaacgcg gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg
    31981 ctggagcaaa atatcattca gccgctggtg ttaagtttgc tgcatctttg ccgtagcgtg
    32041 gtgaatatgc cgccgggcaa ttcgcagccg cgcggcgatt ttctctatca cagcatttgt
    32101 aactgggttc aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt
    32161 aatatcacgc ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc
    32221 gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg
    32281 tcaattcatg aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt
    32341 ttccggcgtc agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt
    32401 cagaaggtta attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac
    32461 aacgactggc ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca
    32521 tgattcatcc cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt
    32581 tctgactgcc agccaatcgg tttcgccagc cgggcaatgc tgatactgga atgacggatc
    32641 cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct
    32701 tcccgctgcc agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg
    32761 aggttgccgc acaggaactg gatcgcctgc tctttattgc taaaatcctg atcaacaaag
    32821 atattctcca gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac
    32881 gcttcaattt cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc
    32941 tcgctatcca gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc
    33001 agctcatcca ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca
    33061 ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg
    33121 cgcaggaacg atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg
    33181 accgcataca gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg
    33241 cagaagtggt cgatgatgta gcacaccgac ggaacttcca ccatgatccc aagcgtaatc
    33301 gtctctgcat gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct
    33361 ttcacccata agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg
    33421 aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg
    33481 taaatacgta ccgcgcgata gccgaggaac gggttctctt cctggggaat attcagataa
    33541 ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc
    33601 gccagtagca cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga
    33661 tccatataga gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg
    33721 cctggcgctt ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg
    33781 gaataggcca gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc
    33841 tgcgcgacct gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac
    33901 tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca
    33961 ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt
    34021 ttctccagaa tcatgcccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt
    34081 aaatcttcag ccaccagaat ggtcggtttt tcaagcacca gcttgttgcg cggcttcagt
    34141 tccggccagg tgatatgcag caactgttcg ctgatatcgc gaatgtcgct aacacgttcg
    34201 cgcagataat cgctggcaga ggcagaaagt ttggcgcaaa cctgctccat attgctgatg
    34261 atcgccgccc ccagcccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat
    34321 tcatcatcct gaatcagcga caaatgggcg ctgaggatag ttttgctttc gccgtcacgc
    34381 tcacgcaatt gctggttcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg
    34441 gaatcttgcg cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg
    34501 gtcagcgtac ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta
    34561 aggcggctta atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt aggctgaacg
    34621 ctgtcgctgt cgataaagcg cacctggatg tactcttcca gcacgcgccg cgcctgctct
    34681 tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa atagggtgcc ggtgccaatc
    34741 agcgccagcg agcttttcgc atctgccttt gcgttctggc gatggttaat aaaagtgatt
    34801 tcgctttgcc actggctgca ctgttcttta agttcccacg ccggacgagc atgtagaccg
    34861 ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg ctccgggtta atcaggagat
    34921 catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa gacgttgaat aagggcagat
    34981 aaaagttgct gcatctgcaa aatgtcgcgg caatcggcaa tcgatgcggc gcaatgtcca
    35041 tggcgggttg ctggccccat caccactgtg ggcacgccgg tgccggttaa gtgcaccgcc
    35101 ccgccgtccg tgccgccgtt gctgaacata tctgcctgca acggcacgcc aatttctgcc
    35161 gccacggttt cgacccaggc ggtaagtttt ggcggcgcaa tcagcgactt gtcgcttaac
    35221 accagcatcg gcccgttacc aatctggcga tggttagccg cgccataatc aaagtttttc
    35281 gcccagcagg cggtatcaag cacaatggcg acgtccggcg acaccgcgcg ggtggcagtt
    35341 tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca ccagccacac ttccgcaggt
    35401 agttcggcgt cgtgcagttc gcgcagtaac gtcaccagca gatagcaacc gaggcggtca
    35461 tcaaaggctt tccccatcac tcgctggtga gggagaacct gaaaagtggt atcaaacgtg
    35521 acgcgatcgc cgggacgaat tcccgcctgc atcacttcgt cataggagcg cgcaccaatg
    35581 tccacgcgca tggcgctgac gtcattcccc tgccggtcgc cgtcaagcag gcctggaatt
    35641 ttgcactctt cacgggtggt gatgcgcacc ggctgcagct ggcgggcagc catgcgtacg
    35701 ttgccaaccg gcagcacatc aatcgcccct tcgcgggaga tgctgcgcac cataaatccc
    35761 acttcgtcca tatgcgcaca gatcatcacc ttcggacctg tcgattcatt caggcggatc
    35821 agcaccgatc ccagaccatc aaatcgcact tctttttgca ggcgatccgc ttcttccagc
    35881 aggatctgcc gcacttcctg ttccgaggag gcgatcgcat ctgcctcgct caacgctttt
    35941 aatagcgata aatccattat gcctctcccg tgagcaacac tgttttcggc atggcgtaga
    36001 gcacttctgc gccttgcggg gtaaccagca caacatcttc gatgcgcacg cccccttgcc
    36061 ctggcaaata aatccccggc tccacggtca gtaacatgcc tggctgtagc gtcgtggtgt
    36121 cccgcggtga aaaacgcgga tcttcatgaa cttcaatgcc gatagcgtga ccggtgttat
    36181 gaccgaaata gtcgccataa cctgcttctg taatgacccg gcgcgcggca tcgtcaacct
    36241 gctggcagcg cacgccgggg cgaatcgcgg agattgctgc gagctgtgcc tgcaggacaa
    36301 tttgatagac gttaaacagc gggtgagatt cggcgctcac cccttcgcca ttcaccagca
    36361 aggtgcgcgt catatcagag cagtagccct gatacagcgc accgaaatcg agagtgacaa
    36421 actcgcccgc tgcaacaatc ttgtcgctgg ctttgccgtg cggcagcgcc ccacgccagc
    36481 cactggcgac aatggtgtca aaagaggctt tttctgcgcc ctgctggcgc ataaaccact
    36541 ccagttcagc ggctatctcg cgctcgctca tccccgcctg aataaagcgg cgaatatgct
    36601 ctgcaccgcg atcagcaatc ccacaggcga ggcggatttt ctccacctcc tctggcgttt
    36661 tgatttgccg cagcacatcc ggcgtggcgc taaccagttt cgcattgagt tcagactgcc
    36721 agcggtgcgc ggtttcccaa ctcacctgct ggccctcaaa accgagcgtt tgcaactgtt
    36781 catcggcaat gatttgattg acgatagtgg taagcgtgtt cgtcgcgtca agcaaatgca
    36841 gctggtagcc ttgcgcacgg gcttccacct caacgtaata gcgcgaatcc accagaatgt
    36901 gcgcactttc acggctaatc accacataac ctgatccggt ggagatcccc agatgcggct
    36961 gtttgttctg ccgtgaggag agaagcactg catccagttg ttgcgcctta agccagtcgc
    37021 gcagcgaagc gagtaatgtc atctggattt tccttcttac aggctatcga ttaacaattt
    37081 gcctttacgg aacatcatca gacgcaggaa caccaccatc agcgcagtta tgactgcccc
    37141 cagcgcgatc cccgccatat agacgcccag gttagtgacc agcggccatg cccagatagc
    37201 ggactccggg aaccattgca ctgcgcccag ccagacagcg gcggttgagc cgacaatagc
    37261 gccgaccata tacgacggaa tggcggtgat ggggctttcc agcgcaaacg gaatcgcacc
    37321 ctcactgatc cccataaacg ccaggaacat ggcggtctta ccctgcggat aaagttgagc
    37381 gttgaacagg cgtttgccgg ttaaacgacg gtcgataatg gtcgccagcc ccagaccaat
    37441 cggcggaata acgatagcga tagaacgagc ggtgaccggt aatacgtggt cagtggtaaa
    37501 gctgaaggca acaaaacctg cggctttgtt gatcgggcca ccaaggtcga ttgctgtcgc
    37561 cgcggcgatc cccattgcgt acatcagcgc gcccttctca cctgcggcag tcagcacagt
    37621 acggataccg ccgttgatcc agccaccaaa gggggtgatg acgtagtaca tcgccagcat
    37681 gacaaaaatg gcggaaagaa tcgggagcaa aaatgtggtt ttgaacgcta acaggaaatc
    37741 cggtaactgg attttctggt tcatccactt cacaaggtaa ccggcaacga tagaaataat
    37801 cagcgcacca atgaaggtgg acggcaccgg cgaagaggtc gcccattgca tagtgctggg
    37861 atcgaagttc agcagttgcg tcggctgggt agacatcaac ccgccgataa aaccagccgg
    37921 gaacgccagt ttgccaccga tagagttcgc cacaaaagca gcaaacatcg gaatggcaaa
    37981 accaaacaac acgccgccga aggactgcga cagccaggca aatttaagta aggagaggtc
    38041 gaaaccggag aatttcccgc tattaagagc atccatgatg ccgatctcag caggaatttt
    38101 aagccagcta taagcaatca gctggctgaa agcgaggatc acaccgccca taattaaggt
    38161 cggcaccatg cgtgaaatcc ctgacatcac atgctgcggg agttcacccc agaagctggt
    38221 tttagaggcc tgcggattct taactgccgc tgccgcaccg gatgcgccag gcacaacggt
    38281 tgcactgcgt tttttaatgg ccataacaat tatccctgtc gaatattact gttgttcaga
    38341 ggcaatcatc tcttcgattt ctttgatgat gcccgcagcg tttttaattg cgtcctgcaa
    38401 agtgatttca taaacgtcgc gtgattcgaa acgttcgtta tcttccgggg taactgccac
    38461 ggagtggatg atgatggtcg cttcggcgat atcctgcgcc gtcaggcgat tctggatacc
    38521 gtccgcgccc tgggtttcaa ttttcacttc ataaccggct tctaccgccg cttcttccag
    38581 cgcctgagcg gccataaagg tgtgagccag gcccatcggg caggcacata aggcaatcag
    38641 tttcttactc atcgttaatt cctcgttcag tgatagttga cgagtgaaat tatgaacgtg
    38701 caaaacaaat cgccgttacc agacaaataa tgcatttact ggagaattaa tcccgctgta
    38761 cggaagtgtg acgcaggtcg accttgtgag gggacacaaa gaaaaattga ataaactgta
    38821 tgatttaaaa gattatcggg agagttacct cccgatataa aaggaaggat ttacagaatg
    38881 tgacctaagg tctggcgtaa atgtgcaccg gaaccgagaa ggcccggatt gtcatggacg
    38941 atgagataca ccggaatatc atggacatat tctttaaagc gccctttatc ttcaaatgcg
    39001 gcacggaaac cggaggcttt gaagaactca aggaagcgcg gcacgatacc gcccgcaata
    39061 aacacgccgc caaatgtccc gagattgagc gccagattgc cgccaaaacg gcccataatg
    39121 acgcaaaaca gcgacaatgc gcggcggcaa tcggtgcagc tgtcagccag cgcgcgttcg
    39181 gtaatatctt ttggcttgag attttctggc aggcggttgt cagctttcac aattgcgcga
    39241 tacaaattca ccagcccagg gccagaaagc acgcgctccg ccgaaacatg accaatttcc
    39301 gcacgcaata tttcgaggat aatggcctct tcttcactat tcggcgcaaa atcaacgtga
    39361 ccgccttcgc ctggcaagct tacccaacgc ttatcgacat ggaccagatg cgcaacccca
    39421 agccccgttc cggcaccgta aaccgcaata ggcttacctt cgaccggttc tgcgccacca
    39481 aactgaatca gatgctcttt tttcagcatc gggatcgcca tcgatacagc ggtaaaatcg
    39541 ttaataattt ccagatggct aaaaccgaga ttctttttca tttcggcaat tgagaacgcc
    39601 caggtatggt tggtcatcgc cacccagtca ccggtaattg ggcaagcgat ggcaatacag
    39661 ccgtctttca cctcgacctt atgttcttca agataaacgc gaatgaccgc ttcgaggctg
    39721 gggtaatcaa gccctgaata ggtcttagcc tgcgagattt caccactggc aatatcacac
    39781 agagcaagac gtgcgttggt gccgcccaca tcaccgacta atgcatactt tgtcattctt
    39841 caactgctcc gctaaagtca aaataattct ttctcacact gtaaatacct gggggcataa
    39901 caacaacgcc ggaaaggcag gctccctgta aatatcgatc tgggtcacac aattacttta
    39961 tcgtttcagc accaattgca gcgatgcctt tttgcaagct gggcaaacta agtatctgac
    40021 cccgcataag gaatagaaca tgctccatcc gcgagccaga accatgttgt tattatcgct
    40081 ccccgccgtg gcaattggga ttgcgtccag tcttattctg attgtggtga tgaaaatcgc
    40141 ctcggtatta cagaatttgc tctggcaacg actgccggga actctgggga tagcccagga
    40201 ttcacccctc tggatcatcg gtgtattaac gctaacgggt attgcggtgg ggttggttat
    40261 ccgtttcagc cagggtcatg ccggaccaga ccccgcctgt gaaccgctga tcggcgcacc
    40321 ggttccgccc tctgcgctac ctggacttat cgtagcatta attctcggtc ttgctggcgg
    40381 cgtcagcctg gggccggaac atccgatcat gaccgtcaat atcgcccttg cggttgccat
    40441 tggcgctcgt ctgttaccgc gcgtcaaccg aatggagtgg actattttag cctctgccgg
    40501 aaccatcggc gcactgtttg gcactcctgt tgcggcggcg ttgatatttt cgcaaacctt
    40561 aaatggcagt agtgaagttc cgctatggga tcgtctcttt gcgccgttaa tggcggcagc
    40621 agctggtgca cttactaccg gattattttt ccatcctcat ttttcactgc ccattgctca
    40681 ttacggacag atggaaatga ccgatattct cagcggtgca attgtcgcgg cgattgccat
    40741 cgcagcaggg atggtcgccg tatggtgctt accacggttg cacgcgatga tgcatcaaat
    40801 gaaaaatccg gtgctcgtgc tgggtattgg cggatttatt ctcggtattc tgggggttat
    40861 tggtggacca gtttcgctgt ttaaagggct ggatgagatg cagcagatgg tggcaaatca
    40921 ggctttcagc accagcgatt actttttgct ggcggtaatt aaacttgccg ccctggtcgt
    40981 tgctgccgcc agtggctttc gcggtgggcg aatcttcccg gcagtgtttg tcggcgtggc
    41041 attagggttg atgctgcatg agcacgttcc cgccgtacca gcggcaataa ccgtttcttg
    41101 cgctattctc ggcatcgtgc tggtggtaac acgcgatggc tggttaagtc tttttatggc
    41161 ggcagtcgtt gtacccaata ccacattgct accgctgctc tgtatcgtca tgcttccggc
    41221 atggctgtta ttagcaggta agccgatgat gatggtcaat cgtccgaagc aacagccacc
    41281 ccacgataac gtttagcaaa aaatgctctc ctttatgatt taagagttat ggcgatgata
    41341 cggggcttta cctccccgta atattgcgtt aacaggccgc tgacggcttc gagcgtgaag
    41401 gagaataaca atgttcaggt cactgtttct ggcggccgcc ctgatggcat ttaccccgct
    41461 tgcagcaaac gcaggtgaaa tcaccctact gccatcaatc aaattacaaa ttggcgatcg
    41521 cgatcattac ggtaattact gggacggtgg tcactggcgc gaccgtgact actggcatcg
    41581 caattatgag tggcgcaaaa accgttggtg gcgtcatgat aatggctacc accgtggctg
    41641 ggataagcgt aaagcgtatg agcgtggcta tcgtgaaggc tggcgcgatc gtgacgatca
    41701 tcgcggaaaa ggccggggtc atgggcaccg ccattaaacg cgtccgccaa tggagcacaa
    41761 tgcctgatgc gacgctagtg cgtcttatca ggcctacaaa ccggctcttt aacgcgtagg
    41821 gcggataagg cgtttacgcc gcatccgcca atagtgccag atgcgacgct cattcaacta
    41881 caatcccagc gccgtcccca ccaacaacca gatattcagc gccacgacca gcaccacaat
    41941 cacccagcct gtctgtttta cgcgtttgct gttcaccaga tcgcccatca acttgctgtc
    42001 actggtgaaa atcagcagtg gaaccagcgc cagagcgata ccaaaactta acagtacctg
    42061 actcataacc agaatccgtg tcggatctaa tcccatcaga atgacaataa atgacggcaa
    42121 catggtgact gtacgacgca cccacagcgg gatatgaaag cgaatgaagc cctgcatcac
    42181 cacctgcccc gccagtgtcc ccaccaccgt tgaagacagc cccgcagcaa ccaggcttaa
    42241 tccaaagacc gttgccgcag cgtggcttaa cagcggttgc agcgtcagat aagcctcatc
    42301 aagatcggca acaccagtat gaccggaaaa gtggaacgcc gccgcagctg tagccatcat
    42361 cgccagattg acaaaaccgg caatagtcat ggcgatagcc acatcccatt tggtggcgga
    42421 ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg agcgaggagt gcaaataaat
    42481 cacatgcggc ataatcgtcg cccctaacac gcctgctgcc aggaagaccg cttccgaagt
    42541 aggtaaactc gggatcacca ttcctttacc cagctgcgcc aggttaggct gggagaaaat
    42601 caactcgaca atgtaagccg cggcaacaaa caacagtaac ccgccaatca ctttctccag
    42661 cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa gtcgcgatcc ccgtcagcac
    42721 cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa ccgatcgccg caccaataaa
    42781 ttccgccagg tcggttgcca tcgcaataat ttctgcctga acccaataga accacactac
    42841 gggacgcgga tagtgatcgc gaatctgctc cgccagattt ttaccggtgg caatccctag
    42901 tttggcagag aggatctgaa tcagcatcgc catcaggttg gcccaaacga caacccacag
    42961 tagctgatag ccgaagctag cacccgcctg aatattggtc gcaaagttac cgggatcgat
    43021 ataaccaatc gccgcaatga acgcaggtcc cattaatgcg agcctcatct tgcgcgccgc
    43081 ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg tgcctctaaa acatagcctt
    43141 tgctatgttt catgctatgc caaacgagaa tgattatcaa attcatttaa atggattgtg
    43201 gtgatttctc tgatagacca ggattatgac tacgaaaaga ttgatgactt gaatgttatg
    43261 gttatgttta atgttagcac atttacataa ctttcagctt ccatacacaa catagcagaa
    43321 atgtatgaca gatcactatt tttgaagcct gtcacaggac gtcattatag tgtgtgtcag
    43381 atctcgtttt ccttaaccat gttacataga atgtgcacgg aaatttaacc tgcctcatat
    43441 ttggagcaaa tatggaccgc gtccttcatt ttgtactggc acttgccgtt gttgcgattc
    43501 tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat ccgttatgtt attcaactgc
    43561 ttgttatcga agtgttactg gcgtggttct tcctgaactc cgacgttggt ttaggcttcg
    43621 tgaaaggctt ctccgaaatg ttcgaaaaac tgctcggatt tgccaacgaa gggactaact
    43681 tcgtctttgg tagcatgaat gatcaaggcc tggcattctt cttcctgaaa gtgctgtgcc
    43741 caatcgtctt tatctctgca ctgatcggta ttctccagca cattcgcgtg ttgccggtga
    43801 tcatccgcgc aattggtttc ctgctctcca aagtcaacgg catgggcaaa ctggaatcct
    43861 ttaacgccgt cagctccctg attctgggtc agtctgaaaa ctttattgcc tataaagata
    43921 tcctcggcaa aatctcccgt aatcgtatgt acaccatggc tgccacggca atgtccaccg
    43981 tgtcgatgtc catcgttggt gcatacatga ccatgctgga accgaaatac gtcgttgctg
    44041 cgctggtact gaacatgttc agcaccttta tcgtgctgtc gctgatcaat ccttaccgtg
    44101 ttgatgccag tgaagaaaac atccagatgt ccaacctgca cgaaggtcag agcttcttcg
    44161 aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc cattatcgtt gccgcgatgc
    44221 tgattggctt tatcgccctg atcgccgcgc tgaacgcact gtttgccacc gttactggct
    44281 ggtttggcta cagcatctcc ttccagggca tcctgggcta catcttctat ccgattgcat
    44341 gggtgatggg tgttccttcc agtgaagcac tgcaagtggg cagtatcatg gcgaccaaac
    44401 tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa aattgcttcc acgctctctc
    44461 cgcgtgctga aggcatcatc tctgtgttcc tggtttcctt cgctaacttc tcttcaatcg
    44521 ggattatcgc aggtgcagtt aaaggcctga atgaagagca aggtaacgtg gtttctcgct
    44581 tcggtctgaa gctggtttac ggctctaccc tggtgagtgt gctgtctgcg tcaatcgcag
    44641 cactggtgct gtaagaccat acataaaaaa gccggggata attccccggt ttttttacgc
    44701 ctgttaatca gctaatggct gcgggcgacc aatcaaatac ccttgcagat attgcacccc
    44761 gagcttatgc aatagcgcct gctgctgttg cgtctcgaca aactccgcga ccacactcaa
    44821 tgacttcgct ttcgccagat cggtaattga tctcacaatc atcgcatcca gcgtgttcgt
    44881 gacaatatct ttcacaaaga cgccatcaat tttgatgata tcagcctgca aacgctttaa
    44941 ccgttcgtag ttggcatatc cggtgccaaa gtcatcaatc gcaatccgga aaccaaactt
    45001 atgcagctgc tcgatgttgt acatgctgct ttctgcgtta gaaaacgcct gctcctcggt
    45061 gatctcaaga atgaccgcct gcggggagat gtgataacgc ttaaacagac gaataatccg
    45121 cccggcaata ttcttttgca gcagcgtgag cggcattaaa ttgactgaaa agcgcggtcc
    45181 ttttttgtcg caagggtgtg ttgccagcca cttcaacaag gattccagca cttgcaaatc
    45241 aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga aacttatccg gggtcataat
    45301 gccgccgtca tatttcagtc gcgcgaggat ctcatcataa ccttcaccct ctttgttgcg
    45361 aattggctgg gcgtagagca gcaaatcacc ctgatctaac gccgtgcgaa tggtattcag
    45421 caatagcacc tgtttagtgg tctgcccgga aaccatctcc tctctgctat ccagcgccag
    45481 cacatgatga tgtgcgcagg attgctccgc cagccagctt aactgcccca acaagggttg
    45541 cagggtttcc tgatttccat caaaacgccc ccaggcagca ccatagccca tatccagccc
    45601 ggtattgttc cagtgaattt gccgactatt caggatgtta accatatgct ggagtcgccc
    45661 ttccgtttcc ggcccgctca gcactaacag cagttcacta cccggcaatt gatacaactt
    45721 ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt gagcggatac agtgaacgcg
    45781 catcattaag ccgtaatgac gactcataaa ctcaagatta tcaatgcgca ggcagcaaaa
    45841 actcttgccc gcctcttgct ccggcgcttg ttccaacgca ctaaagttgg gtaggagcgt
    45901 taacggatct gtcagcgcct gcaaatgcca gcggcgatta agccattcac tgcgatgata
    45961 aatgcgcacc atatagagca ggcaaacgct aaaggaaatc agcaccgcaa gaataaatgc
    46021 cagcgaatat tcggtttcca ccccttgcaa aaagttctgg ttgtaattca gaaggcaaag
    46081 cgttgaaaca gcccaggtga gatttaaaaa cggatagcga agcttaccga ccccgagggt
    46141 aaagatgatg aagaaaacgg gtaccaggta accggcaata aagtcgtttt cataaggtgt
    46201 gcacaacaga agtagcagca cgcttaacgc tgccagccag cttaaggtaa atgcgcgttt
    46261 ctctttgccc aacgacggag cgatatccct gcgccacaat atctgcgcaa agtggggact
    46321 tacaatcatg cgagtgagat agtagaaaag catgttgtaa atcagcacag cggtgaaaag
    46381 gcttagcaaa tcaacgaccg tgaaaatggc atccgcatcg ccgaaaaagg tggatatctt
    46441 tagcgggaaa tcaaagaagc tccccacaag atacatgctg catttgatgc cgatcggcgt
    46501 caccaaacca agccagaaca gacgttgcca gacatatcgg ctggtcaatc cataacgcca
    46561 acgcgtcccc agctgccagc gtaatattgc gcaggcacac aacacgacaa acgtctggct
    46621 accgagcatg accgcagtct gtaaaaaaga gagatgaaag ttccacagat tggtgcaaaa
    46681 catcccgcat aaaacgggaa cgacaccgcg ccagccaaag ataaacaaga ttgacagcat
    46741 tacgcacagt ggcatccacg ccagaaagat ataactggaa tggataatgg caagtggcga
    46801 aataaaacgg gatagctgaa taagtaccac ggtgagcgta aacgctagtg tgaatatctt
    46861 gatatttttt atcaggttat gctccacaaa catgagtaca ctactctcac tgatcggggt
    46921 tttcaggcac acaaataata agtttattcg catcgcgaag caaacagggg gccagcctgt
    46981 tgccgatttt tcatccaatg atgctttcac caggcggcac taacccggac gtagactgta
    47041 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    47101 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    47161 agctgagcta tagccccacg atgcgtttac gtaccaagtt tgctgggtgc aaaatttggt
    47221 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    47281 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    47341 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    47401 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    47461 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    47521 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    47581 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    47641 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    47701 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    47761 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    47821 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    47881 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    47941 ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    48001 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    48061 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    48121 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    48181 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    48241 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    48301 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    48361 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    48421 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    48481 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    48541 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    48601 tttttcgcgg cgtcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    48661 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    48721 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    48781 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    48841 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    48901 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    48961 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    49021 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    49081 ttcagggcct taagaatgtt gatctggcgt ggcgtgttgt tgatatggtc ttcgccacgg
    49141 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    49201 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    49261 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    49321 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    49381 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    49441 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    49501 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    49561 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    49621 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    49681 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    49741 ccttacgttt agaaagatgc cgacaaccgg caaatactgg aaaaattaag tgggcgatat
    49801 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    49861 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    49921 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    49981 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    50041 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
    50101 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    50161 gcggttcgat cccgtcatca cccaccactt tctcgccagc taaatttctt gtaaaaatgt
    50221 gaagtaccga agtgggtgat tagctcagct gggagagcac ctcccttaca aggagggggt
    50281 cggcggttcg atcccgtcat cacccaccac ttcgggtcgt tagctcagtt ggtagagcag
    50341 ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccaa tgtaaaaaag
    50401 cgccctaaag gcgctttttt actatctgcg atactcaaag attcgaacct gcagcaggtt
    50461 tgagttgagc gcagcgaaac aacggagccg ctcgcggcga cggcccgaag ggcgagcgaa
    50521 gcgagtcatc ctgcacgacc caccaatgta aaaaagcgcc ctaaaagcgc ttttttgcta
    50581 tctgcgattt gcgaaattgc ctgatgcgct tcacttagca gactactatt tccggcaatt
    50641 cctgtctcct cacctactgt gtcaatgcag ccaacagctt aaccatcgcg ggcgtcacct
    50701 gctgtgtttc ataaacaata tataaatctg cagggatgcg ctgtttgagc ggacggaaaa
    50761 tgacacctgg ccagttcatt tgtgcgtagc tgtccgctat caatgtgata ccaatgccca
    50821 tactgaccat agcgagtacc gtttgcggtt cattaacttc gcgaataaca accggtgaaa
    50881 atcccacctg ctggcaaact cgctgcaaaa aatcccagtc agtgtaaacg ggcggcattg
    50941 taacaaaata ctcgtcacgt agcgcttcca gcgggacggt ggaaaatgat gagagatgat
    51001 gctcttcagg catcgccacc agaaacgccg attcatgcaa ccgtaagctg gtaaaaccag
    51061 tcggtggttc tgtcgccatt cgccagatcc cggcatcaag ttcgcggcgt tccagcaagg
    51121 ccatttgcat cgcgggcatc ttttcgcgaa aaagaacgtc aacgttagga ttttccctga
    51181 ggaatcgccg cataaccggg cgcatccgtc cccacattgc cgttcccact acgccgagtt
    51241 caatccgccc tgcttctccc cgacctattt gttcaatccg agccaataca ttattagcat
    51301 tcaccagcaa tcgacgcgat tcttccatca agattttgcc cgcgtgtgtc agtacgacgc
    51361 tgcgcgaatg gcgaataaaa agctgcgtgc cgagttgatt ttccagctct ttaatatgaa
    51421 tgctgagcgg aggctgagac atatttaaac gcgctgctgc gcggccaaaa tgcaactctt
    51481 ccgctacggc aagaaaataa cggagcaact taagatctgt tctgtatacg cgttccataa
    51541 ttaagtgctc catataataa atacatcgta ttaaattatc atggcataaa gtatttcacc
    51601 accagatatc cgacatacat aaacacaatg gataatatac ccggtaatac atttggtaca
    51661 ggaataggca agcgcacgat tgtaaacaaa gcaccaatac caaaaccaac gccggaagaa
    51721 aataaaatat ctttcactaa ttaaccttta tcataaaagc agctctgaag agcagagccg
    51781 cgaatccttt taatgagtca ccgctcgatg ctttatcttt tcagggtcat gattatattt
    51841 aaacccaaag aaaaatatca ctgcgagaaa aagagcatat cctgcaaaca ccagccagat
    51901 agtttgccag tcttttacgc catccaccga aaagtaatct actgccatgc cactcagaat
    51961 cgagccaacc catgcgccga caccatttac catggtcata aagagcccct gcgcgctggc
    52021 acgaatgctg gaatcaactt cctgttcgac aaataccgaa ccagaaatat tgaagaaatc
    52081 gaatgcacag ccataaacaa tcatcgacag cagcagcaaa ataaatccgg ttgttgacgg
    52141 atcgccatag gcgaagaagc caaagcgcag cgtccaggcc accatactca tcagcatgac
    52201 ggttttaatg ccaaatcgct ttaaaaagaa tgggatagtc agtataaagc ccacttctgc
    52261 catctgtgaa actgacagta aaatggaggg atatttcacc acaaaactgt cagcaaactc
    52321 cgggttacgg gcgaaatcat gtaggaacgg attaccaaaa acgttggtaa tttgcagtac
    52381 cgcacccagc atcatggcaa agaggaaaaa gatggccatg cgtggatttt taaacagcac
    52441 gaaggcatcc agacccagct tgctggcaag cgatgtggtc gcttttttct ccgcaaccgg
    52501 aatcttcggc aaagtcagcg cataagccga cagcagcaat gacgcaccgg acgcgatata
    52561 cagctgcaga ctactcaatt ccagatgcag caggcttact gcccacatcg cgacaatgaa
    52621 ccccaccgta ccaaaaacgc gaatgggcgg gaaagcggtc accgggtcaa gccctgcctg
    52681 ggcaagacag gaataagaga cgctgttcga taacgcaata gtcggcataa acgccatcgc
    52741 attgactaac atcacccaaa acatcatatc cggatcagtt acggatgccg cataaaaaag
    52801 tacgcccgca cacaccaggt gacacagcat gtatgcacgt tctgcgcgca gccatttgtc
    52861 tgcgatgatc cccattatac caggcataat aatcgcggcg atccctttgg aactgtaaac
    52921 catgccaaca ttagcgccgg tgaaatgaag agtattaatc atgtaagagc cgagggtaac
    52981 cagccagctc ccccagataa aatattgcaa aaaggacatt acctttaagc gcatcgcgat
    53041 gctcataatc gtttccttgt cgcatcattt ggcctcaccc gcgaggccgt ttcttattgt
    53101 ttttctcagg caattttgcg cagaaagcca caaataaggt tgatgaagtt ctgcttagag
    53161 agctccgcag ccgccagcgt ttgcgcatgt gacaatttca catcgcttaa accttccgcc
    53221 atattggtaa tcgcagagac cgcaacgact ttaaggtcgc aatggcgagc tgaaataacc
    53281 tcaggcacca cagacatacc aacaacatcc ccaccaataa tttgcatcat gcgaatttcc
    53341 gccgcagtct cgaaattcgg ccccggatac gagacgaaca cgccctccgt cagagggaaa
    53401 ccctcttctt tcgccacttt ttgtaacagt gcgcggtatt ccgcatcgta ggcatttgcc
    53461 agtgagaaga agcgctctcc aaaacgatca tcgttaagac ccaccatcgg cgtaccgggc
    53521 atggtgttga tatgatcttt caatgcgacc aggctgcctg cccccacttc cggacgcagc
    53581 gagcctgccg cattggtgca gaacagtagc tcgcagccca gcagcttaaa ggtacgaatt
    53641 gcgtcagtca taatggtcat tccacgacct tcgtagaaat gtccgcgacc tttcatacat
    53701 accaccggta cgccctggag atggcccagc actaactccc ccgcatgacc atgtaccgta
    53761 cttaccggga agcccggcag cttttcgtag gaaattgcga cagcgttctc aatctgatcg
    53821 gccagcgcgc cgagcccgga acctaaaata aaggccactc gtggcgtgaa atcaggttta
    53881 taagtcttga taatatctat gcaaaacagc gggttatgag aaaattgaac ctgagacata
    53941 catatccttt ttctgtaggg tggaatctaa cgccttttta tagcgaggtt gtcccggctc
    54001 ttaccaatac acttttctca cgcattcaat agcgattcga tatcgatgga tttatatctt
    54061 ttctc
//
LOCUS       DBBGLQ010000029        53472 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.029, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000029 DBBGLQ010000000
VERSION     DBBGLQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.029"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            14..745
                     /gene="artJ"
                     /locus_tag="ACIYSB_002190"
     CDS             14..745
                     /gene="artJ"
                     /locus_tag="ACIYSB_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGC5429241.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(795..893)
                     /locus_tag="ACIYSB_002191"
                     /pseudo
     CDS             complement(795..893)
                     /locus_tag="ACIYSB_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(944..2071)
                     /gene="rlmC"
                     /locus_tag="ACIYSB_002192"
     CDS             complement(944..2071)
                     /gene="rlmC"
                     /locus_tag="ACIYSB_002192"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGC5429242.1"
                     /translation="MQCALYDAGRCRSCQWIMQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2112..2600)
                     /gene="ybjO"
                     /locus_tag="ACIYSB_002193"
     CDS             complement(2112..2600)
                     /gene="ybjO"
                     /locus_tag="ACIYSB_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGC5429243.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2660..3505)
                     /gene="potI"
                     /locus_tag="ACIYSB_002194"
                     /pseudo
     CDS             complement(2660..3505)
                     /gene="potI"
                     /locus_tag="ACIYSB_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
     gene            complement(3502..4455)
                     /gene="potH"
                     /locus_tag="ACIYSB_002195"
     CDS             complement(3502..4455)
                     /gene="potH"
                     /locus_tag="ACIYSB_002195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGC5429244.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4465..5598)
                     /gene="potG"
                     /locus_tag="ACIYSB_002196"
     CDS             complement(4465..5598)
                     /gene="potG"
                     /locus_tag="ACIYSB_002196"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGC5429245.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5693..6805)
                     /gene="potF"
                     /locus_tag="ACIYSB_002197"
     CDS             complement(5693..6805)
                     /gene="potF"
                     /locus_tag="ACIYSB_002197"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGC5429246.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7156..7632)
                     /gene="ybjN"
                     /locus_tag="ACIYSB_002198"
     CDS             complement(7156..7632)
                     /gene="ybjN"
                     /locus_tag="ACIYSB_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGC5429247.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7720..8622)
                     /gene="rimK"
                     /locus_tag="ACIYSB_002199"
     CDS             complement(7720..8622)
                     /gene="rimK"
                     /locus_tag="ACIYSB_002199"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGC5429248.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8683..9405)
                     /gene="nfsA"
                     /locus_tag="ACIYSB_002200"
     CDS             complement(8683..9405)
                     /gene="nfsA"
                     /locus_tag="ACIYSB_002200"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGC5429249.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLDGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9389..9676)
                     /gene="ybjC"
                     /locus_tag="ACIYSB_002201"
     CDS             complement(9389..9676)
                     /gene="ybjC"
                     /locus_tag="ACIYSB_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGC5429250.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9836..10093
                     /gene="grxA"
                     /locus_tag="ACIYSB_002202"
     CDS             9836..10093
                     /gene="grxA"
                     /locus_tag="ACIYSB_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGC5429251.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10123..10500)
                     /gene="ybjM"
                     /locus_tag="ACIYSB_002203"
     CDS             complement(10123..10500)
                     /gene="ybjM"
                     /locus_tag="ACIYSB_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGC5429252.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10770..12455
                     /gene="ybjL"
                     /locus_tag="ACIYSB_002204"
     CDS             10770..12455
                     /gene="ybjL"
                     /locus_tag="ACIYSB_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGC5429253.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12630..13166)
                     /gene="rcdA"
                     /locus_tag="ACIYSB_002205"
     CDS             complement(12630..13166)
                     /gene="rcdA"
                     /locus_tag="ACIYSB_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGC5429254.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13250..14458
                     /gene="ybjJ"
                     /locus_tag="ACIYSB_002206"
     CDS             13250..14458
                     /gene="ybjJ"
                     /locus_tag="ACIYSB_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5429255.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALFGMMILSLALWLKS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHISLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14458..15273
                     /gene="ybjI"
                     /locus_tag="ACIYSB_002207"
     CDS             14458..15273
                     /gene="ybjI"
                     /locus_tag="ACIYSB_002207"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGC5429256.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDNFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15359..15643
                     /gene="ybjH"
                     /locus_tag="ACIYSB_002208"
     CDS             15359..15643
                     /gene="ybjH"
                     /locus_tag="ACIYSB_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGC5429257.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(15684..16916)
                     /gene="mdfA"
                     /locus_tag="ACIYSB_002209"
     CDS             complement(15684..16916)
                     /gene="mdfA"
                     /locus_tag="ACIYSB_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGC5429258.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYRAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17201..17797
                     /gene="ybjG"
                     /locus_tag="ACIYSB_002210"
     CDS             17201..17797
                     /gene="ybjG"
                     /locus_tag="ACIYSB_002210"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGC5429259.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAVV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            17855..18613
                     /gene="deoR"
                     /locus_tag="ACIYSB_002211"
     CDS             17855..18613
                     /gene="deoR"
                     /locus_tag="ACIYSB_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGC5429260.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18660..19862)
                     /gene="dacC"
                     /locus_tag="ACIYSB_002212"
     CDS             complement(18660..19862)
                     /gene="dacC"
                     /locus_tag="ACIYSB_002212"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGC5429261.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20109..20735
                     /gene="gstB"
                     /locus_tag="ACIYSB_002213"
     CDS             20109..20735
                     /gene="gstB"
                     /locus_tag="ACIYSB_002213"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGC5429262.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20732..21847)
                     /gene="yliI"
                     /locus_tag="ACIYSB_002214"
     CDS             complement(20732..21847)
                     /gene="yliI"
                     /locus_tag="ACIYSB_002214"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGC5429263.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21958..22341)
                     /gene="bssR"
                     /locus_tag="ACIYSB_002215"
     CDS             complement(21958..22341)
                     /gene="bssR"
                     /locus_tag="ACIYSB_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGC5429264.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKVKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22554..23879
                     /gene="rimO"
                     /locus_tag="ACIYSB_002216"
     CDS             22554..23879
                     /gene="rimO"
                     /locus_tag="ACIYSB_002216"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGC5429265.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23926..25254)
                     /gene="dgcI"
                     /locus_tag="ACIYSB_002217"
     CDS             complement(23926..25254)
                     /gene="dgcI"
                     /locus_tag="ACIYSB_002217"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGC5429266.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25262..27610)
                     /gene="pdeI"
                     /locus_tag="ACIYSB_002218"
     CDS             complement(25262..27610)
                     /gene="pdeI"
                     /locus_tag="ACIYSB_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGC5429267.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQRAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTCAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(27788..28699)
                     /gene="gsiD"
                     /locus_tag="ACIYSB_002219"
     CDS             complement(27788..28699)
                     /gene="gsiD"
                     /locus_tag="ACIYSB_002219"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGC5429268.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(28702..29622)
                     /gene="gsiC"
                     /locus_tag="ACIYSB_002220"
     CDS             complement(28702..29622)
                     /gene="gsiC"
                     /locus_tag="ACIYSB_002220"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGC5429269.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(29640..31178)
                     /gene="gsiB"
                     /locus_tag="ACIYSB_002221"
     CDS             complement(29640..31178)
                     /gene="gsiB"
                     /locus_tag="ACIYSB_002221"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGC5429270.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTLNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(31198..33069)
                     /gene="gsiA"
                     /locus_tag="ACIYSB_002222"
     CDS             complement(31198..33069)
                     /gene="gsiA"
                     /locus_tag="ACIYSB_002222"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGC5429271.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(33056..34021)
                     /gene="iaaA"
                     /locus_tag="ACIYSB_002223"
     CDS             complement(33056..34021)
                     /gene="iaaA"
                     /locus_tag="ACIYSB_002223"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGC5429272.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            34225..35460
                     /gene="moeA"
                     /locus_tag="ACIYSB_002224"
     CDS             34225..35460
                     /gene="moeA"
                     /locus_tag="ACIYSB_002224"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGC5429273.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASHLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            35460..36209
                     /gene="moeB"
                     /locus_tag="ACIYSB_002225"
     CDS             35460..36209
                     /gene="moeB"
                     /locus_tag="ACIYSB_002225"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGC5429274.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARYALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(36388..37050)
                     /gene="fsa"
                     /locus_tag="ACIYSB_002226"
     CDS             complement(36388..37050)
                     /gene="fsa"
                     /locus_tag="ACIYSB_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGC5429275.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            37181..38080
                     /gene="ybiY"
                     /locus_tag="ACIYSB_002227"
     CDS             37181..38080
                     /gene="ybiY"
                     /locus_tag="ACIYSB_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGC5429276.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            38086..40518
                     /gene="ybiW"
                     /locus_tag="ACIYSB_002228"
     CDS             38086..40518
                     /gene="ybiW"
                     /locus_tag="ACIYSB_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGC5429277.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            40664..41479
                     /gene="ybiV"
                     /locus_tag="ACIYSB_002229"
     CDS             40664..41479
                     /gene="ybiV"
                     /locus_tag="ACIYSB_002229"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGC5429278.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHQGALNVIQAVLDNTSPFNS"
     gene            41631..42896
                     /gene="ybiU"
                     /locus_tag="ACIYSB_002230"
     CDS             41631..42896
                     /gene="ybiU"
                     /locus_tag="ACIYSB_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGC5429279.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(43132..44724)
                     /gene="ybiT"
                     /locus_tag="ACIYSB_002231"
     CDS             complement(43132..44724)
                     /gene="ybiT"
                     /locus_tag="ACIYSB_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGC5429280.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            44943..45863
                     /gene="ldtB"
                     /locus_tag="ACIYSB_002232"
     CDS             44943..45863
                     /gene="ldtB"
                     /locus_tag="ACIYSB_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGC5429281.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(45922..47040)
                     /gene="ybiR"
                     /locus_tag="ACIYSB_002233"
     CDS             complement(45922..47040)
                     /gene="ybiR"
                     /locus_tag="ACIYSB_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGC5429282.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(47037..47504)
                     /gene="mntR"
                     /locus_tag="ACIYSB_002234"
     CDS             complement(47037..47504)
                     /gene="mntR"
                     /locus_tag="ACIYSB_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGC5429283.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFSQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            47690..47818
                     /gene="mntS"
                     /locus_tag="ACIYSB_002235"
     CDS             47690..47818
                     /gene="mntS"
                     /locus_tag="ACIYSB_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGC5429284.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            48090..49673
                     /gene="opgE"
                     /locus_tag="ACIYSB_002236"
     CDS             48090..49673
                     /gene="opgE"
                     /locus_tag="ACIYSB_002236"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGC5429285.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(49722..50237)
                     /gene="ompX"
                     /locus_tag="ACIYSB_002237"
     CDS             complement(49722..50237)
                     /gene="ompX"
                     /locus_tag="ACIYSB_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGC5429286.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(50290..50355)
                     /locus_tag="ACIYSB_002238"
     CDS             complement(50290..50355)
                     /locus_tag="ACIYSB_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429287.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            50590..51477
                     /gene="rhtA"
                     /locus_tag="ACIYSB_002239"
     CDS             50590..51477
                     /gene="rhtA"
                     /locus_tag="ACIYSB_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGC5429288.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            51776..52279
                     /gene="dps"
                     /locus_tag="ACIYSB_002240"
                     /gene_synonym="pexB"
     CDS             51776..52279
                     /gene="dps"
                     /locus_tag="ACIYSB_002240"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGC5429289.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            52683..53429
                     /gene="glnH"
                     /locus_tag="ACIYSB_002241"
     CDS             52683..53429
                     /gene="glnH"
                     /locus_tag="ACIYSB_002241"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGC5429290.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
BASE COUNT        13439 a        14101 c        12990 g        12942 t
ORIGIN      
        1 cgggagttcc atcatgaaaa agttagttct tgccgcttta cttgcttcct ttactttcgg
       61 tgcttctgcc gcagagaaaa tcaattttgg cgtttcagcc acctatccac cctttgaatc
      121 tataggtgct aataatgaga ttgtcggctt tgatatcgat ctggcaaaag ccttgtgcaa
      181 acaaatgcag gcagaatgta cttttactaa tcacgcgttc gacagcctga tcccgtccct
      241 gaaattcaga aaatatgacg ccgtaatctc cggtatggat atcaccccgg agcgtagcaa
      301 acaggtatcg tttaccacgc cctactatga aaactcagcc gtcgtgattg ccaaaaaaga
      361 tacctacaaa acgtttgccg atctgaaagg caaacgtatt gggatggaaa acggtactac
      421 gcaccagaaa tatattcagg atcagcaccc ggaagtgaaa actgtctctt atgacagtta
      481 tcagaatgcc tttatcgatc tgaaaaatgg tcgtattgat ggggtatttg gtgacacagc
      541 ggtggtaaac gaatggctga aaaccaatcc acaacttggt gttgctactg agaaagtgac
      601 cgatccgcaa tattttggca ccggcctggg catcgctgta cgtccggata acaaagccct
      661 gctggaaaaa ctgaataacg cgctggcagc aattaaagct gacggtactt atcaaaaaat
      721 cagtgaccag tggttcccac agtaagattc agaaagccgc aaccgcggct tgagactgat
      781 gacaaaggta aaattgcctg atgcgctacg cttatcaggc ctacatggcc catgcaatgt
      841 attgaatttg cacgcttttg taggccggat aaggcgttta cgccgcatcc ggcataaaca
      901 acgcgcactt tgtcagcaat ctgaagccgc acatgcggct tttttattgc ttcaccagca
      961 gcgtcagcac ttcatagtgc gcggtatgcg ggaacatatc gaaaagctgc acccgttcaa
     1021 tacgaaaccc aggcagttcg cggatatctt tcgccatagt ttgggcgtta cagctggagt
     1081 agatgataaa acgcggtgcc atcgttgaga gataatcaca cagcggttta ccaatgccgc
     1141 ggcgcggcgg gttaaccagc accagctccg gcacatcccc ctgagcggtg gcaaactgag
     1201 tggagtccag cgcctgaaat tgcaaacgcg ttaagcccag ttcagcggct gactgctttg
     1261 cacaggcaat ggcctctgat gcaatttcga tcccggttaa ctgcatgtca ggcgtcgcgc
     1321 agtgtaaacc aaagcccccc acaccgcaga acagatccca catatgttta accggcagct
     1381 gtcgtaccca gtcgcgcgcg gtggcgtaca actggctggc gaccgccgga ttagtctgga
     1441 agaaactttg cggacggatc cacagcggta cgtcattaaa acgctccgcc agtgcctgtt
     1501 gttcggtcag gtagatctcc gtctcccctt ccataatcgc catatgtacc ggctgaatat
     1561 tgacggtaat aactttcagc tgcggtagtt gttcgtgtaa ccacggcagc gccttacgca
     1621 gttgcgccag cttggtatca gaacgcagta caaagcgcag catcatgcct ccatcactct
     1681 ggctttcagt cagcagaatg tatttcagtt cgccacgctt acgcgccacg ttgtagggcg
     1741 ttaaccccgc tcgggcgata aacggtttta gcgccgcaaa aacgggcgca aatgaggcag
     1801 gataaagcgg gcagtcacaa aggtcttctg gtgtgccatc gcgatgcagc ataccgagca
     1861 gtggtttttc aacgctacca ctcaccacca ttttggcttt attacgaaac ccttgttccg
     1921 ggcctgacac cggcgcgcac cattcctcaa ccggaaagtc ggcgagcaga tttttaagat
     1981 cggcggtttt agcggagagt tgctctggaa tcggctgcat tatccactga caggaacgac
     2041 agcgacccgc gtcgtaaagt gcgcactgca tacattgacc ttcacatcat caggggcgac
     2101 gattatacac attattgcaa ctggaagaac cgccgactgg tcgagggaac gaacagcagc
     2161 atcaggatga gcatatccgg cagcttctgc agcatcaggc tatggaagat ttcacgtttt
     2221 gattcaccgg gaatgctgaa cagctccgga taaccgtacc ccagcgaagc cgcccacaag
     2281 taacttgcgg cggtgatttg tgtcagcaga tatagccagc gcgcccagcg acgccctttc
     2341 accagtgaaa acgcacacca gatctcaatg aaaaccagca ccagactgct taaaaagacc
     2401 agcgttaaac tccaagtttg tacgctgcga tgaatgaact cgccaatacc gcgcacgccc
     2461 agcgtattga aaatcatcag cacgtcgagg ccacggatca taataatggc gagcgccgcc
     2521 acctgcacca gcgcaggcac attcaggcga gcatgagatg aagatgtttt cttaaaaaat
     2581 cccaacgttt cgtcttccat gaaaacaatg ccgcgacatg cgcggcatta tgtagccagg
     2641 ttggcaaatt ttagtgtctt cagccacgtc ttgcacgctg gatgtcgcgt atccgctgtt
     2701 tttctgcgcg agccatcaga taccaggcga taaatccgac aattccgacc gcgccgagga
     2761 tcaatgttgc cagggcgttg atttccggat tcacccccat ccgcacgctg gaaaagacca
     2821 gcatcggtaa cgtggtggct cccggcccgg aaacaaagct ggcgatcacc agatcatcaa
     2881 gcgacaaagt aaaagccagt aaccagccag aaatgatcgc gggcatgatc atcggtagcg
     2941 taatgacgaa aaacactttc agcggcgtcg caccgagatc catcgctgct tcttctatcg
     3001 agcgatccag ttcccgcaat cttgaagaaa taacgaccgc cacataagcc gtacaaaaag
     3061 tgacatgcgc cagccagatg gtgagcatac cgcggtccgc aggccagcca atggcatgag
     3121 caagcgcgac gaataataac aacagcgaca agcccgtgat gacatctggc attaccagcg
     3181 gcgcggtgat cataaaggca aagccatttt atccgcgaaa cctgccaaag cgcaccagca
     3241 ccaccgccgc aatagttccg aggatcgccg ccgccgtcgc cgcacaggcc gcaattgtca
     3301 gacttaaacc aaccgcactc atcatcgcgt catcgcgcaa taactcacca taccagcgcg
     3361 ttgaccagcc ggcccacacc gtcaccagct tcgagctgtt aaacgaatag ataaccagca
     3421 tcagcattgg cgcgtagaga aaggtaaagc ccagcagcaa aatcacaatc cgccagggcg
     3481 aacgaactac cggtaaatta ttcatccgtg ttctcccacg cttttttgct ggtgtttgtg
     3541 gaaccacatt atcggcacaa ttagcagcag caacatgatg atcgctaccg ccgaggccac
     3601 cggccagtcg cggttattga aaaactcttg ccatagcacg cgcccgatca tgatgctgtc
     3661 cgggccaccg agcagttccg ggatcacaaa ctcgcccaca gccgggataa acaccagcat
     3721 cgatccggca ataatcccac ctttagtcag cggcacgatc acagtaaaga acgttttcag
     3781 cggtcgtgca ccgagatcca gcgctgcttc caccagcgaa taatcaatac gaatcaacgc
     3841 ggtataaatc ggcagtacca taaacggcac gtaagcgtaa acaatgccga tataaacggc
     3901 cagattggta tgcagaatgg tcagcggttg atcgataacc cccagccaca gcagaaaatt
     3961 attcagcaca ccgttgtttt ttaatattcc catccaggca taaacgcgga tcagaaacga
     4021 ggtccacgac ggcaggatca ccagtagtaa taaaatatta cgggtcgaag gcttactgtg
     4081 cgccaccgcc cacgccagcg gatagccgat cagtaaacag caaaatgtcg aaatcgcagc
     4141 cacctgtagc gactggagat aagcatcgaa atagagcgga tcgtcggtca gttgcagaaa
     4201 attaccgaga ttaagagtga tggacagttg cccgtcagcc cactccatca gttcggtata
     4261 aggtggaata gcgcgcgcca tctccgccag gcttatttta aagacaatca gaaatggcag
     4321 cagaaacagc aagatcaacc agatatatgg caacgcaatg accagtttgc gcccatgctt
     4381 catttgcagc tgcgacaacc acagcttaaa tccgcccggc ggtttcgact gggcagcagg
     4441 ttcaagtgta ctcatctttg ctccttaaac cgtcagcacc acacagctgt ccacttccca
     4501 gcacaaacgc acttcgtcgc cccaggtcgg taacccttta cgatgacgat gggcgttttg
     4561 tagctgggcg ctaatcatct gcccactttt cagacgaacg tgatacaccg aaagatcgcc
     4621 gagataggca atgtgtatca cctcccccac cgcgaagtta caaccattgg cgggcggctc
     4681 ttcgcaaagc atgatttttt ccgggcgcag cgccacatgt accggcacgt tatcgaccac
     4741 cgaggcatcc gcgtcgactt tcagtggatg caccagcccc ggcgaatcaa gcaccaggcc
     4801 atcttcctga cgctctttga gtacgccttc aaagacgttt accgagccaa taaattcggc
     4861 gctatagcgg gtagtcggat gctcgtagat ctcttccggt tcgccaatct ggacaaattt
     4921 cccacgattc ataatggcga tgcgccccgc catggtcatc gcctcttcct gatcgtgggt
     4981 gaccatcaca caagtcacac cgacgcgctc cagaatatcc accacttcaa gctgcatccg
     5041 gtcacgcagc tttttatcca gcgcgcccat cggctcatcg agcagtaata gtttcgggcg
     5101 cttcgcaagg cttcgggcca gggccacacg ttgtcgctga ccaccggaaa gctgatgcgg
     5161 tttgcgtttg gcgaactcct gcatatgcac caggccgagc atctcattga cccggctggc
     5221 aatttccgct ttcggtagtt tgtcctgttt caggccaaaa gcgatgttct gttccacggt
     5281 catatgcgga aacagcgcgt aagactgaaa catcatattg atggggcgca ggtaaggcgg
     5341 aacctgtgac aaatcgacgc catcaagcat tatctgtccg gcagaaggtt gttcgaaacc
     5401 tgccagcata cgcagcagcg tggacttgcc acagccggat gcgcccagca gcgcgaagat
     5461 ttcacctttg tagatggtca ggctgacatc atccaccgca tgttgaccat cgtaggattt
     5521 ggtcaggttg cggatttcta atagcggcgt cagcgcctta cgggttttcg cctgcgggcg
     5581 agggatagcg tcattcacgg ctgctctccg gcattgaatg tgtgcaagcg caccataatg
     5641 gtgcgaattg ccggcgggtg tggtgcgccc ctccggcatc tacgactgcg gattattttc
     5701 cgctcttcac tttggtccac gcgcgggtgc gcacacggtc gattttcgga tcctgcactt
     5761 tcagagtgaa cagcttcgca cgaacatccg caggcggata aatacctggg ttctcacgga
     5821 cttccgcact caccagcggc gtggctgctt tattggcgtt ggcatagaac acatggtcgg
     5881 aaatatgcgc tactacatcc gggcgcagca ggtaattcag gaactgatag gcttcgtctt
     5941 tgtttttggc atccgcaggc atggcgaata catcaaagaa cgccatcgcc ccttcttttg
     6001 gaatcgagaa cgagacattc acgccattct tcgcttcctt cgcgcggttt gacgcctgcc
     6061 agacatcacc tgcccagccg atagcgacgc aaatatcgcc gtttgccagg tcgttaatgt
     6121 attgagatga atggaaataa cgaatgttcg ggcgcagctt taacagcaga tctgttgccg
     6181 gtccggtgta atcatccgct ttagtgctgt tgggatcttt gccgagataa ttcaacacgg
     6241 tagcaaaaac ttcttctggc gcatccagga aagagacacc gcagcttttc agtttttcca
     6301 gattttcagg tttgaggatc aagtcccagc tatcgacggg cgcgttttcg cccagcaccg
     6361 ctttaacttt atcaacgtta tagccaatcc cggtcgtcgc ccacatatag ggcatagcaa
     6421 atttattgtc gggatcgtgt ttggcgacca gcttcagcag ttccgggtcg agattcttcc
     6481 actccggcaa tttgcttttg tccagcggct ggaaaactcc cgcagtcaac tggcgctcca
     6541 gaaagctggc agatggaacc accagatcaa agccggtact cccggccatt aatttgcctt
     6601 ccagtacttc gttagagtcg aaaacatcgt agacgacttt aataccggtt tctttttcaa
     6661 aattggccac cgtgtccggg gcgatataat cagaccagtt ataaatgtgg agtgtttttt
     6721 gttcagccgc gagcgtgccg acagagacgg ccatcagagc acccgcaacc agacccgata
     6781 gccatttttt atttaaggcg gtcatatctc tttccttctg aaagttcgtt aacaaacgaa
     6841 caaaagttgt cacactgaaa gatatgcaat aaacgtgcat attttttatc ggcgcggtgt
     6901 aagaaaagag aactttctcc cggccagcac tgctcgctat ataaaagcat cgcaagaata
     6961 actttagcca gggtttgaga ctatagctcg gattaaaaaa cggtatttaa caaattttta
     7021 tgcatatttg ctctatgtga taaagccaaa tcgacacaag agaagtgaaa taatctttaa
     7081 gaaaaggtta aaaacagcag ataattttgc agcgcgcagc cactgcacca gcgactgcgt
     7141 gctcgaaagg agttatcagt ggaggaaata gttttcggta acgttgtttt gcccttcatc
     7201 atcagtcggt aacagcagtt gatgggcatt agcttcaaga atgaccatcg aaatctgctc
     7261 ttcgctctga cgcacgaacc atgcaaactg ctcataggtt acgccctgca taacggataa
     7321 agactggcaa accaccagct ttggcagatt atcatcctgc atatcaagaa atgctttcac
     7381 ggtcagcgaa ctggcattga tggcagataa atccgccgcc agcggcaata cggctgaagg
     7441 tcggacttcc gccatggcag aaaacaggat cgtgttatcg atcagatcga ttttggcatc
     7501 aaagacaccg tcgaaattct gcatatgggg cagatgcaga gcctgacagt tatcacattc
     7561 aaaaaaactc atccccaggt catcgagcca ttgacgcagc gtatccagac caggaacgac
     7621 cagcgatgtc ataattttgt acaacctctt tcgataaaaa gaccggcaca gcttacgcaa
     7681 aaagcgcagg caaaaccatg atcagtaatg tgattgcgac taaccacccg ttttcaggca
     7741 atattctgtc gtagcgtggc gttcgatcca gcggatcatt ttacccgcga tgtcgatacc
     7801 ggtggttttt tctattcctt ccagccccgg cgacgcattc acctccatca ccaacggccc
     7861 gcgattagca cgcagaatat ccacaccagc aacgtccagc gccatcgttc gcgcggcttt
     7921 tatcgcgatt tcacgctcct gtggtgtgat actggcgacg cttgccgcgc cgccacgatg
     7981 caaattggaa cgaaaatcgc cctctttcgc ccgccgttca atcgcagcga ccacttcatc
     8041 gccaacaacc agacagcgga tatcgcaccc ttgcgcctct ttgatatatt cctgcaccag
     8101 aatatgcgcg ttcagaccgc ggaaagcgtc aatcacgctt tccgccgcct gacgcgtctc
     8161 cgccagcacg acgccaattc cctgcgtgcc ttcaaccaac ttgaccacca gcggcgcacc
     8221 accgaccatg tcgattaaat cgctggtatc atccggcgaa tgcgcaatgc ccgtgacagg
     8281 caggtcgatg ccctgacgcg ccagcagttg catggaacgc aatttgtcac gcgcccgggc
     8341 aatggcgacc gactcattga gcggatagct ccccagcatc tcgaactggc gcagtgccgc
     8401 cgtcccataa aaggtaatgg cggtgccaat acgcgggatc actgcgtcaa aatggggtaa
     8461 cttgcggcct ttgtagtgaa tagaagacgc cgcaggattt atgttcatgt agcaagaaag
     8521 cggatcaaga atttcaacca ggtgaccgcg ctgtatagcg gcttcacgca gccgcttaca
     8581 cgaatagagc gttccatccc gggacaatat ggcaattttc accctgcacc tctctgtcag
     8641 acctggtcaa aagcccgcgt atcatacatc gacgtggcgg ttttagcgcg tcgcccaacc
     8701 ctgtttgtgc aaataatcca gaataaatgg gcggctttct ttaatgattg ttcggcggat
     8761 atgatcgctc caggtatccc ggcgattatt gctgccacgg gtgaggtaat attccgccag
     8821 ttgctcgtca tactgcgcca gtgcgccttt atccagcggt tgatagctgt tttcatgcac
     8881 caaaatggag gccggtaaac gcggcttaag atccggatta tccgcaggcc agccaaggca
     8941 cagcccaaac agcggcagaa catgctgcgg taatttaagc agtttcgtca ccgcttcaat
     9001 attattgcgc aggccgccga tatatacccc gtccaatccc agcgattccg ctgcgattaa
     9061 tgcattctgc gccatcattg ccgtatcaac gacaccgagc aacagttgtt ccgccaggcc
     9121 gagctgagca tccggacaga tctgtaaatg gcggttaaag tcggcacaga acacccagaa
     9181 ctccgccgct tgcgctacgt gtttttgccc gccggtcagc gtcaccagtt cttcacgtaa
     9241 cgctttgtcg gtaatgcgaa taatgctact gcactgcaaa aaactggaac tggacgtcgc
     9301 acgggcgctg ttaataatcg cctcacgctg cgcttcggaa atgggttcat cagtgaaatg
     9361 gcgaatggag cgatggccac aaataagttc aatggttggc gtcattatct ttttctcttt
     9421 ctgaacgtga atattgcggt ggacggttca tcagctgtgg ggcaagacgt tttgccacct
     9481 gaagaataac caccaccgca gcgggaagca tgagcaaaac accgagaaaa atcatcagaa
     9541 tctgcacttc tggccgagaa aatggctcag gcagcgacag ggagtcgctt accgacagca
     9601 gcgccaccgc cagtagcatc attccgataa attccagtat caacacgcct ttaggcaatt
     9661 taccgatcgc gcgcatacgc ttccctctgc aaagtgagcc ttcagtctaa aacttttcac
     9721 tgtattgtgt ttaacagtta tagcttttag caattaatgc aacaggttaa acctactttc
     9781 agcgaataca ttttagcgtg atcattacag gcataaatct atgaggagag aaataatgca
     9841 aaccgttatt tttggtcgtt cgggttgccc ttactgtgtg cgtgcaaaag atctggctga
     9901 gaaattgagc aatgaacgcg atgattttca gtatcagtat gtagatattc gtgcggaagg
     9961 gatcactaaa gaagatctac aacaaaaggc aggtaaaccc gtagaaaccg tgccgcagat
    10021 ttttgtcgat cagcaacata tcggcggcta taccgatttt gctgcatggg tgaaagaaaa
    10081 tctggacgcc tgatcgtctg acaagccctc gcgttgaggg ctttactgat tttttctgtg
    10141 ctgtggttta aacaaactac tgataaataa gaaacacagt gcccccagcg cacaccagaa
    10201 caccgcgctt agtaaccatg ccagctcttg ccagaatgag cgcgtcggtg aaaaaaacag
    10261 ccgcataatg agcatcgaac agggtgccgc cagcattgcg ccaaacagag gtttcaggac
    10321 ttctctacgc tgtgaaaaga agctggcgac tgctccagga agaatgaaaa atagcaagcc
    10381 gatttcagga tgcccggcag cccgaaaagc gcctttcatg tgcgtcgcca gaaaaaggca
    10441 caccacaatg aagaggacaa aacagcagat tgcccccgcc caacgttgtt tatgtttcac
    10501 tcgttcctcc tgacactgcg tctatcgaac acatttttcg ccagtgtggc gttcagtaag
    10561 ataaagccgc ttcgcattcc atgctaatat aggccaacgc aattcatata gccgttgata
    10621 cctaatgtga ttacactagt aaaatatatt gttactttac tatcgtttag gtgcgctgaa
    10681 tgaatctgcg ccctgaattc tggtaaaaaa cattatcgta aattaccatt tctttcaaca
    10741 gcttactagt aaacaagaag ttagcctccg tgaatataaa cgtcgccgaa ttgttaaatg
    10801 ggaattacat tctgttatta tttgtggtcc tcgcgcttgg gctatgtctc ggaaagttac
    10861 gacttggttc gatccaactg ggtaattcca ttggcgtttt agtcgtatcg ctgttattag
    10921 gccaacaaca tttcagcatt aacaccgatg cgcttaatct tggctttatg ctgtttattt
    10981 tctgcgtcgg ggtcgaagcc ggaccgaact ttttttccat tttttttcgc gatgggaaaa
    11041 attacctaat gttagcactg gtgatggttg gcagtgcgct ggtgatcgcc ttagggttag
    11101 gtaagctgtt tggctgggat attggcctga cggccggtat gttagcaggc tctatgacgt
    11161 cgacaccggt tctggtcggt gctggcgata cactgcgtca ttccggcatg gaaagcaggc
    11221 agctctcact ggcactggat aatctgagcc tcgggtatgc cttaacctat ttaatcggtc
    11281 tggtgagttt gattgttggt gcgcgttact tgccgaaatt gcagcatcag gacttacaga
    11341 ccagcgccca gcaaatcgcc cgcgaacgtg gcctggacac tgatgccaac cgtaaggttt
    11401 atttaccggt gatccgcgcc tatcgcgtcg gcccggaact ggtggcctgg accgacggca
    11461 aaaatctgcg tgaactgggt atttatcgac aaaccggctg ctacattgaa cgtattcgac
    11521 gtaacgggat tctggcaaat ccagacggtg atgccgtgct acaaatgggc gatgaaatag
    11581 cgttggtagg ctatcccgac gcccatgccc gactcgatcc cagcttccgt aacggtaaag
    11641 aagttttcga tcgtgacctt ctcgacatgc gtatcgtcac tgaagaagtg gtcgttaaaa
    11701 accataacgc tgtaggtaaa cgtctcgcac aactgaagtt gaccgatcac ggttgcttcc
    11761 ttaaccgcgt cattcgtagc cagattgaga tgccgataga tgacaacgtc gtgcttaaca
    11821 aaggtgacgt tttacaagtc agcggcgatg cccgccgcgt aaaaaccatc gccgatcgca
    11881 tcggctttat ctcgattcac agccaggtca ctgacctgct ggcattctgc gccttctttg
    11941 ttattgggct gatgatcggg atgatcacct tccagttcag cacattcagt ttcggcatgg
    12001 ggaacgctgc cgggttgtta ttcgccggaa ttatgctggg ctttatgcgt gctaaccacc
    12061 cgaccttcgg ttacattccg cagggtgcat taagcatggt gaaagagttc ggcttgatgg
    12121 tgtttatggc aggcgttggt ctgagcgccg gtagcggtat taataacggc ctgggcgcga
    12181 ttggcggtca gatgttgatt gccggattga ttgtcagtct ggtgcccgtg gttatctgtt
    12241 tcttgttcgg tgcttatgta ttgcgaatga accgcgcgct gttgttcggc gcaatgatgg
    12301 gcgcacgtac ctgcgcgccg gcaatggaga tcatcagtga tacagctcgc agtaacatcc
    12361 cggcgctggg ctatgcgggc acctatgcaa tcgccaacgt cctgctgacg ctggcaggga
    12421 caatcatcgt catggtatgg ccaggattag gataaaactg aagttgccct gaaaatgaaa
    12481 tttttttgca caaccgcaga acttttccgc agggcatcag tcttaattag tgccactgct
    12541 tttctttgat gtccccattt tgtggagccc atcaaccccg ccatttcggt tcaaggttga
    12601 tgggtttttt gttatctaaa acttatctac taccctgcaa ccctctcaac catcctcaaa
    12661 atctcctcgc gcgatagcgg cttacggtcg gtgacaaaat gcagcgtcat cccctcaata
    12721 aacgcatcaa gcgcgcgggc ggttccgggt tcaaaccatt gttcgagcgt ttgctgactg
    12781 cgctgcatcc agttttgcat taccgttttt aatagcggtt ttcggctagc cagcgcgtag
    12841 agctggtaca tcagctccat gttatccggc gttgcaacct gtgagctgta gatcatatcg
    12901 gtgatagcct ggcatgcgcc cggagcatca ctaacatcgc taaaaaatgc ctgatattgc
    12961 cgggacatga tctcagtaaa actgctgaac gcctccagta acaactcatc aattcctgaa
    13021 aaatagtagg tcatcgatcc caacggtacc ccggcaaggg tagcaatttt gcggtgcgta
    13081 acagcatgta ttccgtaaag tttcaccgcc tccagcgtgg cctggataat tttttctcgc
    13141 cgttgcggat cgttagcgcg acgcatgtca tttcctctct ctgcaattgt gtacaaatgt
    13201 acacaacctt gctaatgttg tcttccctct tcttattttg acgtgatcta tgaccgtaaa
    13261 ttcttcacgt aatgcattga aacgccgaac ctgggcgctg tttatgttct tctttttgcc
    13321 aggcctgtta atggcgtcct gggcaacccg tacgcctgct atccgcgata ttctctctgt
    13381 ctcgatcgct gaaatgggcg gtgttctctt tggtctgtcg atcggttcga tgagcggtat
    13441 tctctgctcg gcgtggttag tgaaacgctt tgggacacgt aatgtcatcc tggtcacgat
    13501 gtcctgcgca ttgttcggga tgatgatatt aagtctggca ctctggctga aatcgcccct
    13561 gctctttgcc gttggtctcg gcgtctttgg ggcaagtttt ggttctgcgg aagtggcgat
    13621 aaacgttgaa ggtgccgccg ttgagcgaga aatgaataaa acggttttgc cgatgatgca
    13681 cggtttttat agcctgggca cgctggcagg cgctggtgtc gggatggcac tgacggcctt
    13741 tggcgttccg gcaacggtgc acatttcatt ggcggcgctg gtaggtatcg cgcctattta
    13801 tatcgccatt caggcaatcc ctgacggtac gggcaaaaat gctgccgatg gcacccagca
    13861 tggcgaaaaa ggcattcctt tttatcgcga tatccagttg ctgttgattg gtgttgtagt
    13921 gctggcgatg gcctttgccg aaggttctgc caacgactgg ttacccttat taatggttga
    13981 cggtcacggt tttagcccta cttccggctc gctgatttat gccggtttta ccctggggat
    14041 gaccgttgga cgcttcaccg gcggttggtt catcgaccgt tacagtcgcg ttgccgtggt
    14101 tcgggccagt gcactaatgg gggcgttggg tattgggctg attatttttg tcgatagcgc
    14161 ctgggtcgca ggagtgtctg ttgtactttg gggactgggt gcctcgctgg gcttcccgct
    14221 gaccatttct gccgccagcg ataccggccc cgatgcacca acccgcgtca gtgtggtagc
    14281 aacgaccggt tatctggctt tcctcgtcgg gccgccgctg ctgggctatc tcggcgaaca
    14341 ttatggatta cgtagtgcaa tgctggttgt actggcgctg gttattctcg cggctattgt
    14401 cgcgaaagcc gtcgccaaac ccgataccaa aacgcagacg gcgatggaga atagttgatg
    14461 agcattaaat taattgcggt agacatggat ggtactttct taagcgatca aaaaacctat
    14521 aaccgtgagc ggtttatggc tcagtatcag caaatgaaag cacaaggcat tcgctttgtg
    14581 gtcgccagcg ggaatcaata ttatcagttg atctctttct tccctgaaat tgcgaatgaa
    14641 attgcctttg tggctgaaaa cggcggctgg gtagtgagtg aaggcaaaga tgtttttaat
    14701 ggcgagctgt cgaaggatgc ctttactact gtcgtggaac atttgctgac gcgcccggaa
    14761 gtggaaatta ttgcctgcgg aaaaaatagc gcctatacgc tcaaaaaata tgacgatgcc
    14821 atgaaaacgg tggcggaaat gtattatcac cgtctggaat acgtcgataa ctttgacaac
    14881 ttagaagata atttctttaa gtttggcctg aatctttccg atgaactgat tccacaagta
    14941 caaaaagcat tacatgaggc catcggcgat attatggtgc cagtccacac cggcaacggc
    15001 agcatcgatc tgattatccc cggcgtacat aaagccaatg gccttcgcca actgcagaaa
    15061 ttatggggaa tagacgacag cgaagtggtg gtctttggcg atggcggtaa cgatattgag
    15121 atgctgcgtc aggcaggctt tagttttgca atggaaaatg ccggcagcgc ggtcgtcgca
    15181 gcggcaaaat accgggcagg ctccaataac cgtgaaggcg tactggatgt gatcgataaa
    15241 gtgcttaaac acgaagcgcc atttgaccaa taaaaacggc agcacgcccg gcaacacaat
    15301 acttgaatga aggctgcata attcgcggaa attaacccgc tgcattattt caggcgaaat
    15361 gattatgaaa aattgtctac tgttgggcgc gcttttaatg ggctttactg gtgtggcgat
    15421 ggcgcaaagt gtcaccgtcg atgtgccgag tggatacaaa gtggttgtag ttcctgattc
    15481 agtcagcgtt ccacaagcgg tcagcgtcgc gactgtgccg caaaccgtct atgtggctcc
    15541 tgcgccagct ccggcttacc gcccgcatcc ttatgctcgc catcttgcca gcgtaggtga
    15601 agggatggtt atcgaacacc agattgacga ccatcaccat taattcgaga atgcctgatc
    15661 gcacaaagca gtcaggcatt tttttaccct tcgtgagaat ttcccatctg tttatctttt
    15721 aaaaagataa ccatcagcga cagccacaaa attccgttga caaggttgaa gagattaaac
    15781 tgtccgttgc ccccgttcag ccaggcatgt ttgctgattt caataccaac ggtaaagatc
    15841 agcatttgca gcattcccat cgccgcagaa accgtacctt tactcatatc gctggcaaac
    15901 agggttaatc gcaccagtcc cgcattcgcc agaccaatac cgaaagcata aatacttaac
    15961 ccggcagtca tccataaata cgcgtgcgat gagataaccg ttgccgcagc agcgaccaac
    16021 agaccaatca taatcggcca gccgcccata attatcagcg aacgtacggt gcggcgcgag
    16081 gtcaaacgcg ctaacagcaa gttacccgca attaacgccc cgaaaatagg cacttgcagc
    16141 aagccatatt catagctgct caactgctcg ccggtaatga tgataatcgg cgactgggcg
    16201 atccacgcca gcaatggcag gctaacgaat cccagcgcca gcgcccccgc cacaaagcgg
    16261 ccgttcttca gcaccagctt atagtcacga ccgagttctt tcagtgacag tttctcgcct
    16321 atacgcgtgg cggtttcagg catggctcgt tgcagaccga aaaaggagat cgctgccaat
    16381 gcggcaaaca aaacaaacat cccctcccag ggcagcacat ggatccacgc cgcgcccacc
    16441 agcggaccaa gtagcggagc aatcagcgcc acgttcgcca tcagcgcggt gatcttgata
    16501 caaaccgcct cttcgaagga ttcctgaatt gcggcgtatc ccacagcgcc aatgaaacag
    16561 aggcttatgc cctgcaagaa gcgcaacagg gtgaattgtt caatgttttg cgccagcaat
    16621 attgccagac aggtgatgat aaaccacacc acccccgcca gcatcaccgg acggcgacca
    16681 atacgatccg acagcggccc caggagccat tgtaaaaaca tcccgcccgc cagatatgcg
    16741 gtcattgaag taggaaccca atcaatgccc gcccgatatt gttccaccac ggccaacata
    16801 ccgggttgaa tcatatcgtt gccgatatag gttgaaaatt cgtaaagcac cagacagaga
    16861 gggaaaagta acgcctgacg tccaagcctg gcaccggaag ctaatttatt ttgcatgcaa
    16921 tttcttcgcc aataataatc gcgcagagtt taataaaagc gcagctaacg agaaagcgaa
    16981 ttttgtagct gaaaccacgg ttaagcacat tcttacatta ttgcgagtat agctacgctt
    17041 tctttaagtt ttatttaacc tatgcccgtt acaatcaccc accgtaaaca ggccgcttga
    17101 gggaaataag acgatgccgc tttacccagt ttaacctgca ctttattctc aacgacttgc
    17161 ctgtattggc tcccttttaa tcactttgcg tcgggaagtt atgctggaaa atttgaatct
    17221 ctctctattc tctcttatta acgcgacgcc agactcggct ccgtggatga tctcgttggc
    17281 gatttttatt gctaaagatt tgattaccgt ggtgccgttg ctggccgtgg tactttggtt
    17341 gtgggggctt acagcacaac ggcaactggt gataaaaatc gctatcgcgc tggcggtcag
    17401 cctgtttgtg tcctggacga tgggacatct ttttccgcac gaccgaccct ttgtcgaaaa
    17461 tatcggctat aacttcctgc atcatgcggc ggatgactca ttcccaagcg atcacggtac
    17521 ggtgattttc acctttgcac tggcattttt atgctggcat cgcctgtggt ccggctcact
    17581 tttaatggtg ctggccgtcg tcattgcctg gtcgcgcgtt tatcttggcg tccactggcc
    17641 gctggatatg ctcggtggat tgctggcagg tatgattggc tgccttagtg cccagattat
    17701 ctggcaagcg atggggcata aactctatca acgtctgcaa tcgtggtatc gcgtctgttt
    17761 tgcattaccg atccgcaaag gctgggtgcg tgactgatct ctgctaaaaa gtgtagtatt
    17821 gagcggctcg cttcaataac tattcagagg gattatggaa acacgtcgcg aagagcgtat
    17881 cgggcagctg ctgcaagaat taaaacgcag cgataagtta catcttaaag acgccgccgc
    17941 cctgcttggg gtttcggaga tgacgattcg tcgcgatctg aacaaccaca gtgcgcccgt
    18001 cgttttgctc ggcggctata ttgttctgga accgcgcagt gccagccatt acctgttaag
    18061 cgatcaaaaa tcccgcctgg tggaagaaaa acgccgggcg gcaaaactgg ctgcgacgct
    18121 ggtagaaccc gatcagaccc tcttttttga ctgtggcacc accacgccgt ggattattga
    18181 agcgattgat aatgaaatcc cttttaccgc cgtttgttat tcgctaaata cctttctggc
    18241 gctgaaagag aaaccccatt gccgcgcatt tctgtgcggt ggtgaatttc acgccagcaa
    18301 cgccattttc aaacccatcg attttcagca aacgttgaat aatttttgcc cggatatcgc
    18361 tttttattcc gcggcgggcg tgcatgtcag taaaggcgct acctgtttta atcttgaaga
    18421 gttgccggta aaacactggg ccatgtcgat ggcgcaaaag catgtgctgg ttgtcgacca
    18481 cagtaaattt ggcaaggtgc gtccggcgcg catgggtgac ctgaaacgct ttgatattgt
    18541 ggtgagcgat tgttgcccgg aagatgagta tgtgaagtac gcgcagacgc agcgcattaa
    18601 gttgatgtat taatgacgta taaccggatg acgtttcgcg ccatccggtt atcagaagat
    18661 taagagaacc agctgccgaa ccactgatgg aatttcatca tcacgaaatc ccacatccga
    18721 ccaaagaatc cgccctcttc cacattttcc atcacgatca gcggacgctg ctcaatggat
    18781 ttaccgttaa gctggaaatc aatggtcccg acaacctgac cttttttcag cggtgcggta
    18841 agctgcggtt ccgttaacgt ataactcgct ttcaggtttt tcagctgccc acgcggtatg
    18901 gtcactgagc ccgcttcacc tgccccgaga ttcacttcgc tcttatcacc aaaccagacg
    18961 cgctgagtca caaaggtggc atcaggttta attggcgtca cggtttcaaa gaagcggaaa
    19021 ccccaggtca ataatttctc agactcatta aaacggatac ggtcagtttt cgcccccagc
    19081 actacggaga ttaaacgcat atcgccctgg gtagccgaag caaccagatt atatcccgcg
    19141 cctgccgtgg ttcctgtctt catgccatca acattcagat tgctgctcca cagcagacgg
    19201 ttacggttag gctgacgaat tttgttgaag gtgaactctt tctctttatg aatggcgtac
    19261 tcttccggca catcgtggat caatgcttta cccagcaatg ccatatcgcg cgcggtactg
    19321 aactgccccg gcgcatccag accgtgcacc gtctggaagg tagtgttggt cagacccagt
    19381 tttttggcat aaccattcat caaaccaatg aatgactcct ggctcccggc aacgtaatcg
    19441 gccagcgcaa tacaggcgtc attaccggac tggataatca cacctttgtt caagtctgcc
    19501 accgaaacct gatcgcccgg tttgaggaac attaccgatg aaccacgcag tgccggattt
    19561 cccgtcgccc aggcatcttt accgaccgtc accatatcgg tgagtttaat cttatcggcc
    19621 ttaagcgcct gcccaaccac atagctggtc atgattttag tcaggctcgc gggatccagt
    19681 ttctcatccg cgttgccttc tgccagcact ttaccgctgg cgtaatccat taaaatccat
    19741 gcacgcgcat ccacgctcgg cgcttcaacg gtttgttccg ccgcgaatgc cgttggggca
    19801 aaaaggaata aaaatgcaga acctgctgca agaccacgaa ggagagagga gtattgcgtc
    19861 ataaatgcca cccgagtatc cattcaaaaa aaatacgtta tatcgccgta tgataagttc
    19921 ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag tttttaaagt
    19981 ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt tttttgccag ctgttgctta
    20041 aaagttaact ttacttcacc atcggagtcc tgttcagcct gtggctgcct gtcggaaaag
    20101 gggtgagtat gattacgctg tggggtcgga ataattcaac caacgtaaaa aaagtattgc
    20161 tgacgctcga agaactggaa ctaccttatg agcaaattct cgcgggccgt gagtttggga
    20221 taaatcacga tgctgatttt ctggcgatga accctaacgg gctggtgccg ttgttgcgtg
    20281 acgacgaaag tgatctcatt ctttgggaat caaacgccat tgtccgctat ctggcggcac
    20341 agtacgggca aaaacgtctg tggatcgact caccggcacg tcgtgcggaa gcggaaaaat
    20401 ggatggactg ggcaaaccag acgctcagta atgctcatcg cgggatcctg atgggattag
    20461 tcagaacacc accggaagag cgcgatcagg ccgccattga tgccagttgc aaagagtgcg
    20521 acgccctgtt tgccctgctc gatgcggaac tggcaaaagt aaaatggttc tctggcgacg
    20581 agtttggtgt gggcgatatc gctatcgcac cgttcatcta caatttgttt aacgttggcc
    20641 tgacctggac accgcgtcca aatctgcaac gctggtatca gcaactcact gaacgccccg
    20701 cagtgcgcaa agtggtgatg attcccgtta gctaattgcg tgggctaact ttaagtaatt
    20761 ccccactgga ctcgtcggtg agaacgtata aataaccgtc gggtccagtg cgaacatcac
    20821 gaattcgctg ccctctgtcc gttaaaatac ggccatcttc tgtcactttg tcgccgttga
    20881 cgctcatcac aatgacatct ttatctttca gcgcgccaat aaataatttt tgctgccact
    20941 gggggaattt atcgctgtta tagaaggcca tgccgctcac agcgggcgaa tctttccagt
    21001 aaaaaacagg ttgctcggtc ccggcgacga tctccccttt cgcttccggt atcttaaagc
    21061 ctgaatagtt gattccccag gttgccagcg gccagccgta gtttttgcct ttttgcggga
    21121 tattaatttc atcaccaccg cgcgggccat gttcattcag ccacagtgca ttactccacg
    21181 gattcatcgc cattccttgc ggattacgaa tgccataaga ccagatctcg gcgcgcacac
    21241 cggattcctt tataaaagga ttatcatccg ggatttcgcc ctggtcggtc agacgcacca
    21301 gtttgccctg taatttatcc agatcctgcg ccgtcgggcg ctgattgttt tcgcccagag
    21361 caataaaaag ataaccttta ccgtcgaata ccagccgccc gccaaaatgg ttgccggtag
    21421 acagttttgg catctggcgg aagacggtgc ggaagtcggt cacttttgag agatcatcac
    21481 ttaagcggcc ataacccaca gcagttccgg ctttgccatc atcgccaact tcggaataac
    21541 ttaaccagat gcggcgagac tgagcaaaat caggcgctaa aaccacgtcc agcaggccgc
    21601 cctgcccgtg cgcccaaacg tccggaactc cggaaagcgg cgcagataat ccttttcctg
    21661 cttgccagtg acgcaactcg ccgccgcgca gagtgattaa cataccgtga ttatcgggta
    21721 aaaaggccag tgcccaggga tggtcgagtt tgtcttgcag tacttcgaca tttaccgttg
    21781 caggagccgc ccagagagcg gaagaaagac aaataagggg cacaaggaaa aagaattgtc
    21841 gatgcataac gcctccggat tgactctggc ttaaagcgta gtcagtggag gagataacaa
    21901 attcattttt acaaaaactt aaacatgaag ggggagacgc tttctccccc ttagttttca
    21961 ggccttctca agcatggcgt gcttctgcag gctctggata ctcagcgtta agctcatcag
    22021 acaattttca agcttatcgg cgttgacggt aataacagtc gggcaatcat ggtgcccact
    22081 catcaaacat actgcggctg tcgctaatgc ttcttcagca tgatgaagag cactccactc
    22141 ttcctgatcc agatgaagat tcaaccgcag cgatttatcg tgcagttcgc gattcagttt
    22201 aaaaaagtta tctcgtagat gattgctttc gctgacggac atgtatcctt ttaccttcct
    22261 cagttgcacg tatgcagcga ggtcaacgcg tgcgtcgatc aaccggttca gcagatcgat
    22321 tcgctgtctg tcaacgaaca tacttcgatc ctcctctttc cagccttgtt gcaattaaat
    22381 agtatggcta ttttttgagc aaaatgctgt ccaggatcaa ttcttcacgc cgaattaaca
    22441 aaatttacat ttccttcgcc tccgccagcg aatgagattg cgatacaaat tttatccaga
    22501 actctgtaga atccctgccc tgacctttcc tgtaactgaa tattttttaa gctatgagca
    22561 aagtaactcc ccagccgaaa atcggctttg tttcccttgg ctgtccgaaa aaccttgtcg
    22621 attcagagcg tattctcacc gaactacgca ctgaaggtta tgacgtggta ccgagctatg
    22681 acgatgcgga catggtgatc gtcaacacct gcggctttat tgacagcgcg gtacaagaat
    22741 cactggaagc cattggtgaa gcgttgaatg aaaacggcaa ggtaattgtg accggttgtc
    22801 tgggggcaaa agaagatcag atccgcgaag tccacccgaa agtgctggaa atcaccgggc
    22861 ctcatagcta tgagcaggtt ctggagcacg ttcatcacta cgtgccaaaa ccgaaacaca
    22921 acccattcct gagcctggtg ccagaacaag gtgtgaaact gacgccgcgt cattatgcct
    22981 atctgaaaat ttcggaaggc tgtaatcacc gctgcacctt ctgcattatt ccgtctatgc
    23041 gcggcgacct ggtgagccgt ccgattggcg aagtgttaag tgaagcgaaa cgtctggtag
    23101 atgcgggcgt taaagagatt ctggtgatct cgcaggatac ttccgcctat ggcgttgatg
    23161 ttaaacatcg tactggcttc cacaacggcg agccggtaaa aaccagcatg gtcagcctgt
    23221 gcgaacagtt atcgaaactg gggatctgga cacgtctgca ctacgtttac ccttatccgc
    23281 atgtggacga cgtcatccca ctgatggcag aaggcaaaat cctgccgtat ctggacattc
    23341 cgttgcagca cgccagcccg cgcattctca aactgatgaa gcgtccgggt tctgtagatc
    23401 gccaactggc gcgcatcaaa cagtggcgcg aaatctgccc ggaactgacc ctacgctcaa
    23461 cctttattgt cggcttccct ggcgagacag aagaagattt ccagatgcta ctcgacttcc
    23521 tgaaagaagc gcgtctggat cgcgttggct gctttaaata cagcccggtt gaaggtgcag
    23581 acgccaatgc cctgcctgac caggttccgg aagaagttaa agaagaacgc tggaaccgtt
    23641 tcatgcagct gcagcagcag atttccgccg agcgcctgca agagaaagtg ggccgtgaaa
    23701 ttctggtgat tatcgacgaa gtggacgaag aaggcgcgat tggtcgcagc atggcagatg
    23761 caccggaaat cgacggcgcg gtttatctca atggtgaaac caacgttaag ccgggtgata
    23821 tcctgcgtgt gaaagtcgag cacgccgatg agtacgattt gtggggtagc cgggtttaat
    23881 taattcaggc cgggcaaaat catttgtccg gcctgcgaat aatgattact gccagctaat
    23941 ctgagccctg ccgtttttct tactcttata cagccgctca tccgccagca ttttcaagcg
    24001 ttcgacggtg gtacattcat ttgaatgcgc tacaccgata ctggtactta aattaatgct
    24061 tcccacctgg aaatgaacga tttcttttag aatcgatagc aactcttcca gcgttccacc
    24121 cgtataaatc accgcaaatt cgtcaccgcc gaagcggtag attttacctt tatctaccgg
    24181 gatctgtgat ttaaggattt tcaccacttt catcaacact tcatcgccca gcaaatgacc
    24241 gaaggtgtcg ttaatgtttt tgaatttatc gacgtcaaac aggcacaaat aaccgccttt
    24301 ttccggcagc gcttttaaat cttcgtcaaa ggattttctg cccattgctt tggtcaggtc
    24361 gtccaggaac gccgagttat acatactgta gagttttttg acatcggcag ccgaaaagaa
    24421 aagcccgcca tccagtacgc gttcaaaatg gttattaatg atggcttctt tctccacaat
    24481 accacgatta atcactctgc gtaccagata gaggcaactg gtacccgata tcactgccac
    24541 caatgcggca aagaaaataa tcaatgtaca gacccgcaaa taaacatctt ttttatacgc
    24601 aaatacgtac aatccggtcg gcaaataggc gaagtgctcc atcgcttccg aaacggtgaa
    24661 gatggcgttt ttagcataat attcatcacc ggtcaccggt ttaccgttct taaatagaga
    24721 gaacatcagg ttctcgctgt ccatcaccag cgattttgac agtaaagaaa aacgctccag
    24781 accaagcaaa aatatcagac tacgattgtg agttaccgga tagaggtaat agacgaaggt
    24841 tttaccgtca aaagagataa accctgtatt tttgacatgt cgatacttca caacctgcat
    24901 aatatctgca caataatttt tctcgaactc ctttacctga atacattcgt cactggaaat
    24961 cagataagga ccgacatcgt acaaataaga gggggaaacc gagtcggcgt aagctgtgcg
    25021 caaaccaacc aacagctgtt cctccgtggg atttagcgtg ttgataagat caattgccac
    25081 gctacgccga tattcagcaa tatttgccac cgcaaaagca gtatcaataa cggactgttt
    25141 caggctatac acccgttcct ttaccatttg agtgtagatc ccgcaggcaa ctgctgaaat
    25201 catgataaaa atcagcagac tgactgtaag tacgaactta ttgattctgg acatacgtaa
    25261 actactcttt aactaatttt ccacttttat cccaggcgga gaatggcatg ggtttctggt
    25321 acagataccc ctgagcctga acacatccta atttaattaa ttcgcgcgca acgtcttcgc
    25381 tctctacgcc ttccgcaatc accgggatcc ggttatagcg tgccagatcg aaaataatgc
    25441 gcacaatttt tcccgcagta ccgaaaatat cgccggtaaa ttgcgagtcc acctttatgc
    25501 tgtctggatt aatttcataa aaacgtttca ggtttgacaa accggtgcca aagtcatcaa
    25561 tcgatatccg taccccttgt ttacgcagtt gttcaatatt gagataaaaa tgttcattaa
    25621 ttcgctctat cacggtttca gtgatctcaa cacagaggct gttcgccttc aggccatttt
    25681 ctttcatgat ggtggtaaaa cgctcatgaa agtgcggttc gtttaactgc cagggcgaaa
    25741 cattaatatg cagtaagaaa tcatccttca gcgacgcacg ctgactaaaa tgtctgaatt
    25801 cacccattgc cgttttaata atctgataac ccagctcatt gataaacccg atatcttcag
    25861 caatggtaat gaacttcagc ggtgaaatga tcccccgctc cgcagaaacc caacggcaca
    25921 acgcctccgc gcccagaatg gcgcaggtgt taatatcaac gatcggttga tagacgagga
    25981 atagttcgcc aatttccatt gcatggcgaa tattacgcgc aatccagata ttctcttcta
    26041 tttcgttgag tttcgcgtca tcacagaaaa taaactcgga gaacatatgg tcacggatac
    26101 tggaaatcgc cagtttcagt ttttctgcgt aactccaggc gttttccccc tgcaaaggtt
    26161 cacaaatggc agacttatgg atgcgcagat tgcggtagcc aaagctgctg tcaaaatcgt
    26221 ttaccatcgc tattttacgg gtaaatgtct gtacattttc ccgacacaga agtaaataga
    26281 gatcggcatt gaatgtcacg atatcgcagc attgcgcata tttctcggca attcgctgac
    26341 acaatttcct gacgatttta ttgcgttcat caacaccaaa caccgcttcc acggcttcca
    26401 gaccacacag tttgatgatc cccacagcca gatactgatt attgttggac tcaataatcg
    26461 ccatattttt aataaaaccg ctttcctgat cgtaagagat cagtgagagc aatttatttg
    26521 accgttcaat ttcggcatct ttcatttccg cgatcgaatt tttgatgctg ataatctctt
    26581 tcgcatcgcc ttcgcgaatt ttcagagcct cgatctgatc cgttgagtcg ggcaaaaacg
    26641 ccagcgtatt aaccagctcg gtcatttcag aaaattgccg acggaacaca cgagtggtca
    26701 tgaaatatac caccagcaac acgataataa tgaacgcaaa cggtagataa aaaagtttat
    26761 ctgatgcgtt caataaatta ccgccaggat aataaaaata gatttcgaac aatgcattat
    26821 ttggcactgc tgatgtcttt gcaatatact ccgtatcgac agaatcaata atattataat
    26881 taccttcgct attataaata atcagttttt cacgagcgcg ttgattcttg gcaatgatct
    26941 catgaccttg tttcaaaaat agaacttcat cattagcgct gatttgatca agatactcgt
    27001 tcagataccg ccagtttaaa tcgacatgaa ttttatctac cgctaacaaa tcgtgatctt
    27061 tgccgttgat acgtagcgta aaagagatac attttttacc cggacgatca aagcaattgt
    27121 atgccggatt ccagctgttg ttagagtcat ctgaagccac cgaccacggt cgtttcgatg
    27181 ggttaaaatc ggcaaaaata acccgccctg actcgtcgac aagcggtcca tttacttgtt
    27241 cctgatgata aacaacgatt ttatcattcg ccctccgtga aaagagataa tatttattat
    27301 catcaagtac cagtcccacc gagtctaaaa atggcatcat ttccattttc agaatcacgg
    27361 cgcgttgaat ctcctctgta ttttttaata aatacagtga gtcctttaat gtcagggccg
    27421 aacgttcaat atctttaaag aaatagccaa cgtttttctg aataagttca aagcggctat
    27481 cggcacgttt taccgcgcgt tccgatacca gttgatagtt aatgatgaaa aaaagaccaa
    27541 taaatgacag tgctgccaat aaaaataaaa ttatcagcct tatctttatc ttttcgtata
    27601 aacttagcat gttaatacat ttaacaaaat aactatctga acgctgtgcc gctgcaaaaa
    27661 tagtcccttg ctgcggcttt ttaaccaaat tacccgattc tacaaaatca gcagaaagtt
    27721 acaacacttg cttagggcaa taatttcctg tgaggtgatt accctttcaa gcaatattca
    27781 aacgtaacta tcctttaatt ttcggatcca gcgcatcgcg taaaccatcg cccaacaaat
    27841 tgaacgccag tacggtcaga aaaatagcca gggccggaaa aacagcgaca tgcggcgcga
    27901 taaccatatc cgctcgtgcc tcattgagca ttgctcccca ctctggtgtc ggcggctgcg
    27961 caccgaggcc gagaaatgac aggctggcgg cagagataat cgaggtgcca atgcgcatgg
    28021 tgaaaaacac cacgatagac gagacggtcc caggcaagat atgacgcaat aaaatggtca
    28081 tatcgctggc accaatactg cgtgctgact caataaaggt ttgctgtttc aacaccagcg
    28141 tgttgccgcg caccagtcgg gcaaacgcgg ggatggaaaa aatggcgact gcgataatca
    28201 cgttagcaat gccgcttccc aacaccgcaa caacagcgat cgccagtaaa atacccggga
    28261 aggcaaacag cacatcgcaa atgcgcatga tcaaccgatc ccaccagcct tcataatatc
    28321 cagccaacaa gcccagcaac gtcccgatcg ccgcaccgat aaacacggca aacacgcccg
    28381 ccgccagcga gatttgcgca ccaaccagga cacggctgaa aatgtcgcgc cccagtgaat
    28441 cgacgccaaa ccagtgctgc aaagaaggtc cgttattcag attgtcataa tcaaaataat
    28501 tttcggcgtc atagggagcg atccagcgtg caaaaatggc caccacaatc aataaaataa
    28561 cgaataatgc ggcggtcatc gccatatgct gacggcgaaa tcgtcgccag aattcatgcc
    28621 acggtgtacg cacctggtca ggtttgacca gtggcatggc gtttaacacc gcctgacgtc
    28681 gccagttaaa tagtcgcatc cttacttgta cctgatagcc gggttaatgg cggcgtaaag
    28741 cacatccacc actaagttga taagaataaa ttccagcgag aaaagcagaa tttccgcctg
    28801 aatcaccggg taatcgcgca tttctacgga gtcaacgagt aagcgtccaa gtccgggcca
    28861 gttgaaaact ttctccacca cgatagaacc accgagcaaa aagccaaact gtaagcccat
    28921 catggtcact accgggatca tcgcgttacg tagcccgtgt ttgaggacaa cccaggtttc
    28981 gctcacccct ttcgccctcg cggtacgcat ataatcttcg cttaaaacat cgacaaacga
    29041 cgcgcgggta aagcgcgcca tcacggctgc cactgccgcg ccgagcgtca gggagggtaa
    29101 aatgtagtgc tgccaactgt ctgctcccac ggtaggcagc cagcccagtt caacggagaa
    29161 tacctgaatt aaaagcatcc ccagcgcaaa tgccggaaac gagatccccg acaccgcaat
    29221 ggtcatactc aatcgatccg gccaacgatt acgccagacg gcggcgataa ttcccgccgc
    29281 cataccaaat atcaccgccc agaccatact ggttatggtc agccacagcg ttggcataaa
    29341 gcggctggca atctcatcgg caaccggacg acgcgacacc atcgacaggc caaaatcccc
    29401 ctgcacagca ttgctgatat agtgccagaa ctggtgatac agcggctgat ccaaccccag
    29461 ctgctgacgc accagttcta taacctgcgc atcagcttcg ggcccggcaa tcaatcgcgc
    29521 cggatcgccg ggcagcatat gcacaaataa aaacaccagc accgagacga taaacagcgt
    29581 cggaatcaac cccagtaagc gtttgataac gtaattaagc attccactcc ctgcgttgct
    29641 tattgcaaat ccgcgtcttc aaagctgaag ccggtgtctg gcataatcca aaaaccggtc
    29701 aggtttttac tgtgtgccga caccagtttt tctaccacca gcgggatcca cggcgactct
    29761 tgccagatga tatcctgcgc cgccttatac aagcgggtct tttccgccgg atcattagtt
    29821 ttcagtgcct gagccaggaa gtcgtccacc tgtttattgc tgtaaaatgc ggtattaaac
    29881 agcgtcggtg gccagttttg cgaggcgaac agtggcgata gcgcccagtc cgcttcgccg
    29941 gttgaagccg accagccagt gtagaacatc cgcacgccgc tctctttttg ccctttacct
    30001 tcaacttctg ccgcccgctg tccggcatcc atcgcggtca cctgggcttt aatcccgacc
    30061 tgcgctaact gctgctgggt aaattgcagc actttctgcg cggtgctgtg gttatgtgac
    30121 gaccacagcg tggtactgaa accgttggga tatcccgcct ctttcagtaa ttcgcgcgct
    30181 ttcactggat cgtaaggcca cggtttataa ctttgcgcgt aggcgatact tggcggtacc
    30241 acaccagtag ctggcgttgc atagcccgca aaggcaactt tcaccagcgc cggacggtta
    30301 atggcgtaat tcagcgcctc acggaccttc gggttatcga acggcttttg cgtcacgttc
    30361 atactgatat aacgctgcat aattgacgga ctggccatca actcgatatt tttgtttttc
    30421 tccagcagtg tggcctgctc gtagggaatg gggaaagcaa actgcgcttc accggtttgc
    30481 agcattgccg cgcgggtgtt gttatccgcc accggacgcc aggttatgct gtccagtttg
    30541 ggcaatcctg gctgccagta acccgcgaat tttttcacct tcacaaaatc ggtctgattc
    30601 aaggtatcca gttcatacgg tccggttccc accggatgaa aaccaatctc cttgccatat
    30661 ttttccagcg ctgccggtga aatcatcgcg gtcgccggat gggcaagaat attaataaac
    30721 gctgagaacg gctgtttgag ggtaatcttt accgttgtcg gatcgatcgc ctccgttttg
    30781 gcaatattct tatacaggtt atagcgttta agatgattcg ccggatcgct ggcccggtcc
    30841 agattcgctt ttaccgccac tgcgttgaaa tcggtgccat cctggaattt aattccttcc
    30901 cgcaatttca cgatgtaagt aatgccgtca tcggaaacgg tataactctc cgccagcacg
    30961 tttttcagtt tcatctcttt atccagaccg aacagcccct ggtaaaacga tttcgctacg
    31021 gcctgagata acgtgtcatt tgcgtcatac ggatcgagcg tggtgaaatt cgatcctacc
    31081 gccaccacca catctttggc agcgaatgca caagatgcca tcaacgctgt cgcaatgccc
    31141 agcgccacta acccactacg gtgtacagct cttgccattt tattctccgc ctgaatgtta
    31201 tctacgcatg aatgcgtatt ctgattgtgg ttgtgcgacg taatgccccg gcccgacgca
    31261 ttgcaacgag acggctgcca cctcttcgcc acgcagatga atattgctgg gaagatcgtc
    31321 cgacagcagt acacgctgcg gtcgttgtcg ggacggttca gcgaccggaa ctgccgccag
    31381 taatttacgc gtataaggat gctgcgggtt ttcgaagacc gcgcgccgtg gaccaatttc
    31441 aacaatttgc ccgagataca tcaccgccac acgatgacta atccgctcta ccacggccat
    31501 atcgtgggag ataaacagat acgcaatgcc gaaatcacgc tggagatcga gcaacaagtt
    31561 gataatctgc ccgcgaatag aaacatccag cgccgaaacg gcttcgtcgg caatgatcac
    31621 ttttggattc aatgccaacg cgcgagcaat gcagatgcgc tggcgctgac cgccggaaaa
    31681 ctcatgcggg taacgccagg catgttcagg taacaggccc acgcgctcca gcaaccacgc
    31741 aacgcgtgca gccgcgtctt tacctggcaa taaaccgtgt acacgcagcg gttcgataat
    31801 cgaatcaccg atggtctgac gtgggtccag cgaagcgtaa gggtcctgaa aaataaactg
    31861 aatatcccgg cgcaatgcct gaagtttgcc gggtgacaag gtatcgattc tctgaccgtt
    31921 aaagataatt tcgccgccct gcgattcgac caggcgcagc aacgcccgcc cggtagtgga
    31981 tttaccgctg ccagactcgc ccaccagcga tagcgtttcg ccaggccaga gatcaaaact
    32041 gactttctca acggcatgca cttcccgcgt tacgcgattc aacaaaccgc tgcgcaaagg
    32101 gaaacgggtg acaagattac gcactcgtaa aacaggttcg ccatccacca ccgttttctg
    32161 ctcgatgggg ggggcctgtt tcgctggatg ttcaagcgat atcaacggga aacgtcgggg
    32221 ataatctaac cctttcatcg caccaagttg cggaacagca gctaacagcg cacgggtgta
    32281 aggatgttgc ggtgcatgaa aaatctgttc gacggtaccc gtttccaccg cctcgccctg
    32341 atacatcacc agtacccgat cggcaatctc tgccaccacg cccatatcgt gagtgataaa
    32401 gataacgccc atcgacatct ctttttgcaa tactttgatt aattgcagga tctgcgcctg
    32461 aatagtgaca tccagcgcgg tggttggctc atcggcaatc agcaccgccg ggcggcatga
    32521 cagcgccatc gcaatcatca ctcgctggcg catcccgcca gagagttgat gcggataacg
    32581 tgaaagaatg gtttgtgcct caggaatgcg tacctgatcc agcatccgct tcgcctcgac
    32641 catcgcttct tcacgactgg cgttctgatg cagacgaatt gattcggcaa tctgttcacc
    32701 cacagtaaat accgggttca gcgatgtcat cggctcctga aatatcatcg ccatatccgc
    32761 accgcgaaca tggcgcattt gtgcagcgct ctgctcgcta agttcaatca cttcgcgact
    32821 gcgccgctgc aacagcattt tatcgcactg tactaaaccg cccgcctgtt ccaacaggcg
    32881 catcaatgcc aacgcagtca ctgacttacc ggagccggat tcgccaacaa ttgccagcgt
    32941 ctcaccgcgt tgcagactaa aagagagatt gcggaccgca gctattttct gctggtcctg
    33001 cataaaggca atattcagat tttcaaccgc cagcacatta ccggcatcaa gttcatcact
    33061 gtgtggcaac ggtgtcccct ttttcacggt agataccggt ggttggcgta tcgcctgcgt
    33121 agccccaggc gcgatacatt ccttcggtgt taaacggtag cgcgacattc ccttcatggt
    33181 cgatagcgat taagccaccg ctaccgccaa gcgcagggag tttttccatt actacccgct
    33241 cgcaggcttc cgcgagactt aatccgccgt aatccattaa cgcggcgatg tcatatgccg
    33301 ccagcgcgcg gatgaagact tcgcccgtgc cggtacaaga aaccgccaca ctggcgttat
    33361 tggcgtagca tccggcaccc actaagggac tatcgccaac tcgtccgggt aatttattgg
    33421 tcattccgcc tgtggacgtg gctgccgcca aattgccgtc taaatccaac gccacggccc
    33481 ccacggtgcc cattttctgt ttttcatcca gtggcgcacc gctatggtcg aggacggttg
    33541 ccccttcctt gcgcgctgcc agtagttgtt cataacgcaa aggcgtggag aaaatctccg
    33601 gcgagacgcg ctccatgcca cgagcaaacg caaaattttc tgccccttcg ccaatcatca
    33661 tcacatgcgg gctttgctcc atcaccagcc gggcggcaag aaccggatta cgcagatgac
    33721 taacgcccgc caccgcaccg gctttcaggg tgttaccatc catcacacag gcgtccagtt
    33781 catgggtttc atcacgcgta aagacagcgc caattccggc gttaaacagt ggacactctt
    33841 ccagcagacg caccgcttcc gtcaccacat ccagcgcact ttcgcccgct tccagcattt
    33901 tctgcccggt ttcaacaatg gcagacaacg cctcaatgta gcgtaattcc tgttgcagac
    33961 tcatctgcgc gcggctaatt gcacctgcgc caccatgaat tgcaatgact gctttgccca
    34021 tagtattcgt ccattaaggg tggctcgcca cttttgctat aaatatcaga atttttatga
    34081 attactcatt caatttttga atatagaaag atggatatgg catgtaaagg caggagccaa
    34141 cgctacgccc gaggtggcat acagtctgtg gcgtttttct gacataatag gcaaattcga
    34201 ttttgcctcc gaaggagtgt tttcatggaa tttaccaccg gattgatgtc gctcgacacc
    34261 gcgcttaatg agatgctttc tcgcgtcacc ccactgaccg cccaggaaac gctgccactg
    34321 gtacagtgtt ttggtcgtat tctggcgagc gatgtcgttt cgccgcttga tgttccgggg
    34381 tttgataact ccgcaatgga cggctacgcg gtgcgtttag ccgatattgc ctccaggcaa
    34441 ccgctgcccg ttgccggtaa atcctttgcc ggtcagccgt accatggtga atggcctgcg
    34501 ggtacctgca ttcgtattat gaccggtgcg ccggtgccgg aaggctgcga agcggtggtg
    34561 atgcaggagc agactgaaca aacggacaat ggcgtgcgtt ttactgctga agtgcgtagc
    34621 gggcaaaata ttcgccgtcg cggtgaagat atctctgcag gtgcggttgt tttcccggcg
    34681 ggaactcgcc tgactaccgc tgaactgcca gtgattgctt cactggggat tgccgaagtt
    34741 ccggtgattc gtaaagtgcg tgtagcgctt ttttctaccg gtgatgaact ccagttgccc
    34801 ggtcagccac tgggcgatgg ccaaatctac gataccaacc gtctcgccgt acacctgatg
    34861 ttagaacagt tgggatgcga ggtaatcaac ttagggatta tccgcgacga tccacatgcc
    34921 ctgcgcgccg catttattga agccgacagc caggcggatg tggtgatcag ttccggcggt
    34981 gtttcagtgg gtgaagcgga ttacaccaaa accattcttg aagagctggg ggagatcgcc
    35041 ttctggaagc tggcgattaa accgggtaaa ccgttcgcgt tcggtaaact ttctaatagc
    35101 tggttctgcg gcctgccggg caacccggtt tcagcgacgc tgaccttcta tcaactggta
    35161 cagcctttgc tggcaaaact aagcggcaac accgccagcg gcctgcccgc gcgccagcgc
    35221 gtacgcacag cgtcccacct gaaaaaaacg ccaggacgtc ttgatttcca gcgcggcgtg
    35281 ctgcaacgca acgccgacgg tgaacttgaa gtgacgacca ccggacatca gggttcacat
    35341 atatttagct cctttagtct cggcaactgc tttatcgtgc tggaacgcga tcgcggcaat
    35401 gtggacgtgg gcgaatgggt ggaagtagaa ccgtttaacg cgttgttcgg aggcctgtaa
    35461 tggcggaact cagcgatcag gagatgctgc gctacaaccg gcaaatcatt ctgcgcggtt
    35521 ttgattttga cggtcaggag gcgctaaaag attctcgtgt gctggtagtc ggcctcggtg
    35581 gactcggctg cgcagcttcg cagtatctgg caagcgccgg tgtcggcaac ctgacgctgc
    35641 tcgacttcga cacggtttcg ctctcgaatc tgcaacgcca gacactgcac agtgatgcca
    35701 cggtcgggca accgaaggtg gaatccgccc gttacgccct gacgcggatc aacccacata
    35761 tcgcgattac gccagtcaat gcactgctag atgacgcaga acttgcagca ttgattgctg
    35821 aacacgatct ggtgctcgac tgtacggata acgttgcggt acgtaatcaa ctgaacgcag
    35881 gctgttttgc cgcgaaggtg ccgctggttt ccggcgcggc aattcgtatg gaagggcaaa
    35941 tcacggtctt cacttatcag gacggcgaac cgtgctatcg ctgccttagc cgtttgtttg
    36001 gcgagaacgc attaacctgc gtagaagcag gcgtgatggc accgttgatc ggcgtaattg
    36061 gttcgttgca ggcaatggaa gcgatcaaac tgctggcagg ttatggcaaa ccggccagcg
    36121 ggaaaatcgt catgtacgat gcgatgacct gtcagtttcg cgaaatgaaa ctgatgcgca
    36181 atccggggtg tgaggtgtgc gggcagtaat tgccacctcc aggcctgata agacgcgaca
    36241 agcgtcgcat caggcatttg ctccagacgc cagatgcggc gtgaacgcct tatctggcct
    36301 acggttcggg cacagatttc caagagcggc aggcatcggt gcactccgcc acgtcatcag
    36361 caatttatga ggatgcagaa cgtccggtta aatcgacgtt ctgccaaacg ctcccagcca
    36421 gtcctgttca aacttcgcca cagcggcttc caccgccgga tagctaatca tctgttgtgc
    36481 cacatccagt ggcagcgtaa ttgattcaca tcctgccagt aagcagtcca gcgcctgacg
    36541 cggggttttg aaactcgctg ccagcacttt cgcctgcggc gcatgcattt tcaataactg
    36601 gtgtaagtcg gtcacagtct gaatgccgct accgccctga gcatcaatac gattaacgta
    36661 aggcgcaaca tattccgcac ctgccagcgc cgacagcagc ccttgtgctg cgccatatac
    36721 cgcggttccc agcgttggaa tcccttccgc ttttaacatc ttaatagctg ccagcccctc
    36781 ggcggtcacc ggaactttca ccacgatatc cgcaataata gaacgcaatt tacgtgcgtc
    36841 attcaccatc ccttcagcag tggtagccat tacctgggca aacagacgcc cctgaccgcc
    36901 catcgcttca tgaagttgcg gaagcacaac atccagcggt tttttacccg cggcgataat
    36961 gcttgggtta gtggtcacac ccgccagcgg aaaaatacgt gacagcgcct tcaccgcaac
    37021 aacgtctgaa gtatccagat acagttccat aaccatcctc aaaatgtact ttttgtctga
    37081 atgtagacta tcacgggaaa actctctgaa cagttgacgc acatcaatct aactttcatt
    37141 cgaaagtatt ttaatgtttg aacgaaacag agaggcaact atgattttca atattcagcg
    37201 ctactcgacc catgatggcc ccggtatccg cacggtcgta tttcttaaag gctgttcgct
    37261 gggctgccgc tggtgtcaga acccggaaag ccgcgcccgc acgcaggatc tgctgtatga
    37321 cgcacgactg tgtctggaag gctgcgagct gtgcgctaag gccgcgccgg aagtgattga
    37381 gcgcgcgctg aatggtttgc ttattcatcg ggaaaagtta accccggagc atctgacggc
    37441 gttaaccgac tgctgtccga cacaggcatt aaccgtgtgt ggtgaagtga aaagcgttga
    37501 ggagatcatg acgaccgttc tgcgcgataa accgttttac gatcgcagcg gcggcggttt
    37561 aacgctttcg ggtggtgagc cctttatgca gccggaaatg gcgatggcgc tactgcaagc
    37621 cagccacgag gcaggcattc atactgcggt agaaacctgt ctgcatgtgc cgtggaaata
    37681 tatcgcccct tctctgccct atatcgatct gtttcttgcc gatttaaaac acgttgccga
    37741 cgcgccgttt aaacagtgga ccgacggtaa cgccgccaga gtgctggata acctgaaaaa
    37801 actcgccgca gcgggcaaaa aaatcattat ccgcgtgccg ctgattcagg gctttaatgc
    37861 cgacgaaacc tctgtaaaag ccattaccga ttttgccgcc gacgagctgc acgttggcga
    37921 aattcatttt ctgccctacc acacgctggg catcaacaaa tatcacttac ttaatctgcc
    37981 ctatgacgcc ccggaaaaac cgcttgatgc gccagaactg ctcgactttg cccagcagta
    38041 tgcctgccag aaagggttaa ccgcgacctt acgaggataa caaccatgac cacactgaaa
    38101 ctggacacgc tcagcgaccg cattaaagcg cacaaaaatg cgctggtgca tattgtgaaa
    38161 ccgccagtct gtaccgagcg cgcgcagcac tataccgaga tgtatcaaca acatctcgat
    38221 aagccgatcc cggtacgtcg cgcgctggca ctggcgcatc acctggcgaa tcgcaccatc
    38281 tggatcaaac acgatgagtt gatcattggc aaccaggcaa gcgaagttcg cgccgcgccg
    38341 atcttcccgg aatatactgt ctcgtggatc gaaaaagaga ttgatgatct ggcagatcgt
    38401 cccggtgctg gctttgcggt gagcgaagag aacaaacgcg ttctgcatga agtgtgcccg
    38461 tggtggcgcg gtcagaccgt acaggatcgc tgctacggca tgtttaccga tgagcaaaaa
    38521 ggtctgctgg cgaccggaat cattaaagcg gaaggcaata tgacctccgg cgatgcgcac
    38581 ctggcggtga atttcccgct gctgctggaa aaaggtcttg atggtctgcg tgagaaagta
    38641 gcggaacgtc gctcgcgcat caatctgacg gtgctggaag atttacatgg cgaacaattc
    38701 ctgaaagcga ttgatatcgt gctggtggca gtcagtgaac acattgaacg tttcgctgcc
    38761 ctggcgcgtg aaatggctgc taccgaaact cgcgaaagcc gtcgcgatga actgctgacg
    38821 atggcagaaa actgcgatct tatcgcccac cagccgccgc agactttctg gcaggcgctg
    38881 caactgtgtt acttcatcca attgattttg cagatcgaat ctaacggtca ctcggtatcg
    38941 tttggtcgta tggaccagta tctctacccg tactatcgcc gcgacgttga actcaaccag
    39001 acgctggatc gcgaacacgc catcgagatg ctgcatagct gctggctgaa actgctggaa
    39061 gtgaacaaga tccgctccgg ctcacactca aaagcctctg cgggaagtcc gctgtatcag
    39121 aacgttacca ttggcggaca aaatctggtt gatggtcaac caatggatgc agtgaatccc
    39181 ctctcttacg cgatcctcga atcctgcggt cgcctgcgtt ccactcagcc taacctcagc
    39241 gtgcgttacc atgcaggaat gagtaatgat ttcctcgacg cctgcgtaca ggtgatccgt
    39301 tgcggcttcg ggatgccggc gttcaacaac gacgaaatcg tgatcccgga atttattaaa
    39361 ctcggtattg aaccgcagga cgcttacgac tacgcagcga ttggttgtat cgaaaccgcc
    39421 gtcggtggca aatggggcta tcgctgtacc ggcatgagct ttatcaactt cgcccgcgtg
    39481 atgctggcgg cgctggaagg cgggcgtgat gccaccagcg gcaaagtgtt cctgccacaa
    39541 gaaaaagcgt tgtcggcagg caactttaac aatttcgatg aagtaatgga cgcgtgggat
    39601 acgcaaatcc gttattacac gcgtaaatca atcgaaatcg aatatgtcgt cgacaccatg
    39661 ctggaagaga acgtacacga tattctctgc tcggcgctgg tggatgactg tattgagcga
    39721 gcgaaaagta tcaagcaagg cggcgcgaaa tatgactggg tttctggcct gcaggttggc
    39781 attgccaacc tcggcaacag cctggcggca gtgaagaaac tggtgtttga acagggtgcg
    39841 attggtcagc aacagctggc tgccgcactg gcggatgact tcgatggcct gactcatgag
    39901 caactgcgtc agcggctgat taacggtgcg ccgaagtacg gcaacgacga tgataccgtc
    39961 gatacgctgc tggctcgcgc ttatcagacc tatatcgacg aactgaaaca gtaccataac
    40021 ccacgctacg gtcgtggtcc ggttggcggc aactattacg cgggtacgtc gtctatctcc
    40081 gctaacgtac cgtttggcgc gcagactatg gcaacaccgg acgggcgtaa agcccacacc
    40141 ccgctggcag aaggcgcaag cccggcttcc ggtactgatc atcttggccc tactgcggtc
    40201 attggctcgg tgggtaaact gcctacggca gcgattctcg gcggcgtgtt gctcaaccag
    40261 aaactgaatc cagcaacgct ggagaacgaa tctgacaagc agaaactgat gatcctgctg
    40321 cgcaccttct ttgaagtgca taaaggctgg catattcagt acaacatcgt ttcccgcgaa
    40381 acgctgctgg aggcgaaaaa acatccggat cagtaccgcg atctggtagt gcgtgtcgcg
    40441 ggctattccg ccttcttcac cgcgctctct ccagacgcac aggacgatat catcgcccgt
    40501 actgaacata tgctgtaatc tcctcggccc ggcggcgtcg tcgccgggct aaatcattca
    40561 catcatcaat ttcatcctta ctttcattcg aaatataatt tgtgctctgc gtcacattgt
    40621 tattacactg ttacggtttc attttcgagc caggagcaca cctatgagcg taaaagttat
    40681 cgtcacagac atggacggta cttttcttaa cgacgccaaa acgtacaacc aaccacattt
    40741 tatggcgcaa tatcaggaac tgaaaaagcg cggcattaag ttcgttgtcg ccagcggtaa
    40801 tcagtattac cagcttattt cgttctttcc tgagctaaag gatgagatct cttttgtcgc
    40861 ggaaaacggc gcactggttt acgaacatgg caagcaactg ttccacggcg aactgacccg
    40921 acatgaatcg cggattgtta ttggcgagtt gctaaaagat aagcaactca attttgtcgc
    40981 ctgcggtctg caaagtgcat atgtcagcga aaatgccccc gaagcatttg tcgcactgat
    41041 ggcaaaacac taccatcgcc tgaaagctgt aaaagattat caggagattg acgacgtact
    41101 gttcaagttt tcgctcaacc tgccagatga acaaatcccg ttagtgatcg acaaactgca
    41161 catagcgctc gatggcatta tgaaacccgt caccagtggt tttggcttta tcgacctgat
    41221 tattcccggt ctacataaag caaacggtat ttcgcggtta ctgaaacgct gggatctgtc
    41281 accgcaaaat gtggtagcga ttggcgacag cggtaacgat gcggagatgc tgaaaatggc
    41341 gcgttattcc tttgcgatgg gcaatgctgc ggaaaacatt aaacaaatcg cccgttacgc
    41401 taccgatgat aataatcatc aaggcgcgct gaatgtgatt caggcggtgc tggataacac
    41461 atcccctttt aacagctgac ctctctaccg gagccgtgcg ctccggtttt cccttctttt
    41521 tacatcctgt acatcacatc aaatcattaa acttgcatta acttatttat aacttatatt
    41581 gtcacatgca aatcagaatg gtttcgaatg aaagttaatg agaggtggtt atggcttcta
    41641 cttttaccag cgacacattg cctgccgatc acaaagcagc tatccgtcag atgaagcacg
    41701 cgctgcgggc gcagcttggc gacgtccagc agatctttaa tcagctaagc gatgacattg
    41761 ccacgcgagt ggctgaaatc aacgcactca aagcacaggg cgatgccgtc tggccggtgc
    41821 tgtcttatgc cgatatcaaa gcaggtcatg ttactgcaga gcagcgcgaa cagattaaac
    41881 gtcgcggttg tgcggtgata aaaggccatt tcccccgcga acaagcgcta ggctgggatc
    41941 agtcgatgct ggactatctg gaccgcaacc gctttgacga ggtctacaaa ggccccggcg
    42001 ataatttctt cgggacgctc agcgcttcac gtcccgagat ttaccccatc tactggtcgc
    42061 aggcgcaaat gcaggcccgc cagagtgaag aaatggcgaa tgcgcagtcg tttctcaatc
    42121 gtctgtggac atttgaaagt gatggaaagc aatggtttaa cccggatgtg agcgtcatct
    42181 accctgaccg tatccgccgc cgtccgcccg gaacgacctc caaaggtctt ggagcgcata
    42241 ccgactccgg ggcgctggaa cgctggctgc ttccagcgta tcagcgcgtt ttcgccaacg
    42301 tctttaatgg caatctggcg caatatgatc cctggcatgc ggcacatcgt acggaagttg
    42361 aagagtacac ggtggacaac accaccaaat gttccgtgtt tcggacattc cagggctgga
    42421 cagcgctctc tgatatgctg cctggtcagg ggttgctgca cgttgtgccc attcctgaag
    42481 ccatggcgta cgtactgtta cgtccgctgc ttgatgatgt gccggaggat gaactgtgcg
    42541 gcgtagcgcc cggaagagtg ttgccggtat cagagcaatg gcatccactg ttaattgagg
    42601 cgttaaccag cattccaaaa ctcgaggccg gagactccgt ctggtggcac tgcgacgtca
    42661 tccattccgt tgcccccgtt gaaaatcaac agggttgggg caacgtgatg tacattcctg
    42721 cggcaccgat gtgcgagaaa aatcttgcct acgcgcacaa ggtgaaggcc gcactggaaa
    42781 aaggcgcatc gccgggcgac ttcccgcgcg aggactatga aacaaactgg gaaggacgct
    42841 ttacgcttgc cgacctcaac attcacggta agcgagcgtt gggcatggat gtttgagagt
    42901 taatggaagt gaattgatgt gggattaaga aaatcgattc gacgttactc agattgctga
    42961 caagtgcgct ttcttaatgc ctgatgcgct acgcttatct ggcctacaaa gtcctgcaga
    43021 ttcaatatat tacaggtact gcgtaggcct gataagcgta gcgcatcagg ctattttgca
    43081 tttgtcatca gccgccgtca ccccgacagt atcggggtga cggctaatca cttactcgat
    43141 ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc gttccggggt
    43201 gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg aaacaaagat
    43261 cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt ccatatccag
    43321 gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa
    43381 cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga tgtcgtcctg
    43441 gctgaacagc aaacgaccga gaatgctgcg tacagcctgc tcgtcatcgc cttcctgctt
    43501 ccactgactc atccattcga acacggtcag atcattttca aactcatatt cgtggtcctg
    43561 ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt ccggttgcag
    43621 atcgcccacc agcgttttca gcagcgttga tttaccgacg ccgttggtac ccagtaccgc
    43681 aagtttttca ccgacctcca gcagcaagtt gagattttta aacagtggac cgttatcaaa
    43741 ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa
    43801 acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat
    43861 ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa agcggctaac
    43921 gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca gcagacgttc
    43981 acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa cgcgcagctc
    44041 gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga
    44101 gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc
    44161 gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggatcag caaacagcgc
    44221 ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg
    44281 ctgttccact ggaattccca cgccaagcaa caattcaccg gcgcgagctt ccgcagagta
    44341 accgtccatt tcgccgtatt taacttccag atcggccact ttatagccgt cttcctcact
    44401 catttccggc aaagcataga tgcggtcacg ctcctgcttc acttcccaca actctttatg
    44461 ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag
    44521 cttaccaatg cgctcgttgg gatcgaggga aacgttaccc aaagtcggct ctaagtcgcc
    44581 gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg
    44641 gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat
    44701 ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca
    44761 ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa
    44821 ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat
    44881 gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta
    44941 acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca
    45001 gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga
    45061 atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgcggagt
    45121 accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga
    45181 aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag
    45241 gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg accaacaccg
    45301 ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc aactggacca
    45361 ctaaagttga gcgtaagaaa gcaggcccga cctggacgcc gaccgccaaa atgcacgcag
    45421 agtaccgcgc tgcgggcgaa ccgcttccgg ctgtcgttcc ggcaggtccg gataacccga
    45481 tggggctgta tgcactctac atcggtcgcc tgtacgctat ccatggcacc aacgccaact
    45541 tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat
    45601 tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag
    45661 cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg tctaccaccg
    45721 aagcccagtt tgaaggtcag gaaattgtgc caattaccct gacgaagagc gtgcaaacag
    45781 tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgattaaa aaccgctccg
    45841 ggatgccggt tcgtctgaat taatcttcaa aacttaaagc aaaaggcgga ctcataatcc
    45901 gcctttttta tttgccagac cctagttggc cgggagtata actaacaaaa catatcccac
    45961 caacgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg
    46021 atcgttcgcc atacgtagcg caatcaaatt tgccagcgat ccgggtaata acccaaagcc
    46081 acctacgttt accgcccata ccagtaataa agacggcggc acatagttca gcaacaatat
    46141 ggtactcggc acgttactga tcacctgcga taaaccgatt gccgttaacc ataacccggg
    46201 ttcagataga tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa
    46261 atggacgtcg ataaacatcg ccataaacac cagcagcagc gtccagtcca cactgagcac
    46321 cacgcgacgt gccagcagcg caaagcctgc cgccacaatc accagtcccc acagctcttg
    46381 tttgaactcc agcgccgtca gaaagacgat atacagcccc agacaactcc acaccagccg
    46441 cggtttccac tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca
    46501 acaccagcac aggagcagga gcgtcagcat cattgcgcca gccagcggtg ccatttgggc
    46561 aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc
    46621 aattggcgtc agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt
    46681 aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc
    46741 gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag
    46801 ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct
    46861 taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc
    46921 gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac
    46981 aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat
    47041 ttggcaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg
    47101 tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag
    47161 aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc
    47221 gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta
    47281 agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga
    47341 gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg
    47401 cgccgatgcg cctctctgac ctggctgaac ccttcaacgt gctcttccac gttcactaac
    47461 tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct tcctcattat
    47521 ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt
    47581 acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta
    47641 atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt
    47701 caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc
    47761 tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttccg ataaatagcg
    47821 gcgtcgcggt acgccgcttc actcctgctt tcatgcaggc ataacgcgtt ttggtctgaa
    47881 aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt
    47941 gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat
    48001 gcggccccct ttccactctt cacgcactct tgcaggtatt gacccttgac gccagggtaa
    48061 gcacatggcg tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta
    48121 cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta
    48181 acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc
    48241 ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg
    48301 tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc
    48361 tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt
    48421 acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa
    48481 aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg
    48541 cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag
    48601 atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga
    48661 ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg
    48721 ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg tgaaagcgcg cgtcgcgatg
    48781 ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt
    48841 tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt ggcttaacgc
    48901 tcaatcgcgt tgtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc
    48961 gcgcgggctt ccagacctgg tggttttcca accagggtca aatcggcgaa tacgataccg
    49021 ctatcgccag catcgccaaa cgagcagatg aagtgtactt cctgaaagaa ggtaattttg
    49081 aagcagataa aaacaccaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc
    49141 aagagcactc gcaaccgcag ctgattgttc tacatctgat gggctcacat ccgcaggcct
    49201 gcgacaggac acaaggaaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc
    49261 tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca
    49321 gcggcagcag cttctcgctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg
    49381 gtaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt
    49441 ttatggtcat ttccagcgac gataaagcgc atcgtgtgat taaagcccgc cgctcagcca
    49501 atgacttctt aggctttttc tcccagtgga cggggattaa agcgaaggaa attaacatca
    49561 aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac
    49621 agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa
    49681 aatccgcccc gagaggcgga ttttttatat caccaaagtg attagaagcg gtaaccaaca
    49741 ccggcaatcc aggtgcctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc
    49801 agagcaacgt tttccatcgg gttgaactgc agacccgcac cgtaggagaa accgtagtcg
    49861 ctggtgtcgt gtttgtaggt cgggtattca gtggtctgga atttaccata acccacaccc
    49921 actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg
    49981 tagtactggt ttttgttgta gtcaccagag cttgcagtac ggcttttctc ggtgtaagtg
    50041 aaagaaccga tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa
    50101 ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacagta
    50161 gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga
    50221 catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata
    50281 tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc atttcatgta
    50341 acagaacgtt tccatacacc gctatccatc taaatttaaa tcactttttc agagaactgc
    50401 gtaagtatta cgcatgtttt ccctgtcatt catccagatt attcctaatc accagactaa
    50461 tgattccatc aatcctggcg cattttagtc aaaacggggg aaaatttttt caacaaatgc
    50521 tcaaccagca ttgggtatat ccagtacact ccacgcttta cttaagtcta gatatttgtg
    50581 ggagaaagga tgcctggttc attacgtaaa atgccggtct ggttaccaat agtcatattg
    50641 ctcgttgcca tggcgtctat tcagggtgga gcctcgttag ctaagtcact ttttcctctg
    50701 gtgggcgcac cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat cctcatcgcg
    50761 ttctttaagc catggcgact gcgctttgcc aaagagcaac ggttaccgct gttgttttac
    50821 ggcgtttcgc tgggtgggat gaattatctt ttttatcttt ctattcagac agtaccgctg
    50881 ggtattgcgg tggcgctgga gttcaccgga ccactggcgg tggcgctgtt ctcttctcgt
    50941 cgcccggtag atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt cctgctaccg
    51001 ctggggcaag acgtttccca tgtcgattta accggctgtg cgctggcact gggggccggg
    51061 gcttgttggg ctatttacat tttaagtggg caacgcgcag gagcggaaca tggccctgcg
    51121 acggtggcaa ttggttcgtt gattgcagcg ttaattttcg tgccaattgg agcgcttcag
    51181 gctggtgaag cactctggca ctggtcggtt attccattgg gtctggctgt cgctattctc
    51241 tcgaccgctc tgccttattc gctggaaatg attgccctca cccgtttgcc aacacggaca
    51301 tttggtacgc tgatgagcat ggaaccggcg ctggctgccg tttccgggat gattttcctc
    51361 ggagaaacac tgacacccat acagctactg gcgctcggcg ctatcatcgc cgcttcaatg
    51421 gggtctacgc tgacagtacg caaagagagc aaaataaaag aattagacat taattaaatt
    51481 tacatttctg catggttatg cataaccatg cagaatttct cgctactttt cctctacacc
    51541 gtctttatat atcgaattat gcaaaagcat atttattccg aaaattcctg gcgagcagat
    51601 aaataagaat tgttcttatc aatatatcta actcattgaa tctttattag ttttgttttt
    51661 cacgcttgtt accactatta gtgtgatagg aacagccaga atagcggaac acatagccgg
    51721 tgctatactt agtctcgtta attactggga cataacatca agaggatatg aaattatgag
    51781 taccgctaaa ttagttaaat caaaagcgac caatctgctt tatacccgca acgatgtctc
    51841 cgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc agtttattga
    51901 tctttctttg attaccaaac aagcgcactg gaacatgcgc ggcgctaact tcattgccgt
    51961 acatgaaatg ctggatggct tccgcaccgc actgatcgat catctggata ccatggcaga
    52021 acgtgcagtg cagctgggcg gtgtagctct ggggaccact caagttatca acagcaaaac
    52081 cccgctgaaa agttacccgc tggacatcca caacgttcag gatcacctga aagaactggc
    52141 tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag cgaaagatga
    52201 cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg gataaattcc tgtggtttat
    52261 cgagtctaac atcgaataaa tccatcgctg atggtgcaga actttagtac ccgataaaag
    52321 cggcttcctg acaggaggcc gttttgtttt gcagcccacc tcaacgcact tatttagtgc
    52381 atccatctgc tatctccagc tgattaagta aattttttgt atccacatca tcacacaatc
    52441 gttacataaa gattgttttt tcatcaggtt ttacgctaaa taatcactgt gttgagtgca
    52501 caattttagc gcaccagatt ggtgccccag aatggtgcat cttcagggta ttgccctata
    52561 aatcgtgcat cacgtttttg ccgcatctcg aaaaatcaag gagttgcaaa actggcacga
    52621 ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa aaaggaaatg
    52681 ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac cctggctttt gcggtttctt
    52741 ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt ccgtttgaat
    52801 ttaaacaggg cgataaatat gtgggctttg acgttgatct gtgggctgcc atcgctaaag
    52861 agctgaagct ggattacgaa ctgaagccga tggatttcag tgggatcatt ccggcactgc
    52921 aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat caccgacgag cgtaaaaaag
    52981 cgatcgattt ctctgacggc tactacaaaa gcggcctgtt agtgatggtg aaagctaaca
    53041 ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg aagagcggta
    53101 ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt cagttcccga
    53161 acatcgataa cgcctatatg gaactgggca ccaaccgcgc agacgccgtt ctgcacgata
    53221 cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa gcggtaggtg
    53281 actctctgga agcgcagcaa tacggtattg cgttcccgaa aggtagcgac gagctgcgtg
    53341 acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac gaaatctaca
    53401 aaaaatggtt cggtactgaa ccgaaataat aacgctacac ctgtaaaacg cactggcagt
    53461 tccctctccc ct
//
LOCUS       DBBGLQ010000030        53408 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.030, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000030 DBBGLQ010000000
VERSION     DBBGLQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.030"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            113..541
                     /gene="uspC"
                     /locus_tag="ACIYSB_002242"
     CDS             113..541
                     /gene="uspC"
                     /locus_tag="ACIYSB_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGC5429291.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(548..1972)
                     /gene="otsA"
                     /locus_tag="ACIYSB_002243"
     CDS             complement(548..1972)
                     /gene="otsA"
                     /locus_tag="ACIYSB_002243"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGC5429292.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1947..2747)
                     /gene="otsB"
                     /locus_tag="ACIYSB_002244"
     CDS             complement(1947..2747)
                     /gene="otsB"
                     /locus_tag="ACIYSB_002244"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGC5429293.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2914..3900)
                     /gene="araH"
                     /locus_tag="ACIYSB_002245"
     CDS             complement(2914..3900)
                     /gene="araH"
                     /locus_tag="ACIYSB_002245"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGC5429294.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3915..5429)
                     /gene="araG"
                     /locus_tag="ACIYSB_002246"
     CDS             complement(3915..5429)
                     /gene="araG"
                     /locus_tag="ACIYSB_002246"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGC5429295.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5499..6488)
                     /gene="araF"
                     /locus_tag="ACIYSB_002247"
     CDS             complement(5499..6488)
                     /gene="araF"
                     /locus_tag="ACIYSB_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGC5429296.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7285..7788
                     /gene="ftnB"
                     /locus_tag="ACIYSB_002248"
     CDS             7285..7788
                     /gene="ftnB"
                     /locus_tag="ACIYSB_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGC5429297.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7867..8118)
                     /gene="yecJ"
                     /locus_tag="ACIYSB_002249"
     CDS             complement(7867..8118)
                     /gene="yecJ"
                     /locus_tag="ACIYSB_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGC5429298.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8233..8319)
                     /gene="azuC"
                     /locus_tag="ACIYSB_002250"
     CDS             complement(8233..8319)
                     /gene="azuC"
                     /locus_tag="ACIYSB_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGC5429299.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8582..8905
                     /gene="yecR"
                     /locus_tag="ACIYSB_002251"
     CDS             8582..8905
                     /gene="yecR"
                     /locus_tag="ACIYSB_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGC5429300.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9076..9573
                     /gene="ftnA"
                     /locus_tag="ACIYSB_002252"
     CDS             9076..9573
                     /gene="ftnA"
                     /locus_tag="ACIYSB_002252"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGC5429301.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9611..9850)
                     /gene="yecH"
                     /locus_tag="ACIYSB_002253"
     CDS             complement(9611..9850)
                     /gene="yecH"
                     /locus_tag="ACIYSB_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGC5429302.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10041..11252
                     /gene="tyrP"
                     /locus_tag="ACIYSB_002254"
     CDS             10041..11252
                     /gene="tyrP"
                     /locus_tag="ACIYSB_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGC5429303.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLVLSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11314..11979)
                     /gene="yecA"
                     /locus_tag="ACIYSB_002255"
     CDS             complement(11314..11979)
                     /gene="yecA"
                     /locus_tag="ACIYSB_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGC5429304.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12175..12261)
                     /locus_tag="ACIYSB_002256"
     tRNA            complement(12175..12261)
                     /locus_tag="ACIYSB_002256"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12225..12227),aa:Leu,seq:taa)
     gene            complement(12274..12347)
                     /locus_tag="ACIYSB_002257"
     tRNA            complement(12274..12347)
                     /locus_tag="ACIYSB_002257"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12313..12315),aa:Cys,seq:gca)
     gene            complement(12402..12477)
                     /locus_tag="ACIYSB_002258"
     tRNA            complement(12402..12477)
                     /locus_tag="ACIYSB_002258"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12442..12444),aa:Gly,seq:gcc)
     gene            complement(12629..13177)
                     /gene="pgsA"
                     /locus_tag="ACIYSB_002259"
     CDS             complement(12629..13177)
                     /gene="pgsA"
                     /locus_tag="ACIYSB_002259"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGC5429305.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13234..15066)
                     /gene="uvrC"
                     /locus_tag="ACIYSB_002260"
     CDS             complement(13234..15066)
                     /gene="uvrC"
                     /locus_tag="ACIYSB_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGC5429306.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15063..15719)
                     /gene="uvrY"
                     /locus_tag="ACIYSB_002261"
     CDS             complement(15063..15719)
                     /gene="uvrY"
                     /locus_tag="ACIYSB_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGC5429307.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            15861..15977
                     /locus_tag="ACIYSB_002262"
     CDS             15861..15977
                     /locus_tag="ACIYSB_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5429308.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            16015..16191
                     /gene="yecU"
                     /locus_tag="ACIYSB_002263"
     CDS             16015..16191
                     /gene="yecU"
                     /locus_tag="ACIYSB_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGC5429309.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16178..16402
                     /gene="yecF"
                     /locus_tag="ACIYSB_002264"
     CDS             16178..16402
                     /gene="yecF"
                     /locus_tag="ACIYSB_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGC5429310.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16470..17192)
                     /gene="sdiA"
                     /locus_tag="ACIYSB_002265"
     CDS             complement(16470..17192)
                     /gene="sdiA"
                     /locus_tag="ACIYSB_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGC5429311.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRSSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(17422..18174)
                     /gene="tcyN"
                     /locus_tag="ACIYSB_002266"
     CDS             complement(17422..18174)
                     /gene="tcyN"
                     /locus_tag="ACIYSB_002266"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGC5429312.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(18171..18839)
                     /gene="tcyL"
                     /locus_tag="ACIYSB_002267"
     CDS             complement(18171..18839)
                     /gene="tcyL"
                     /locus_tag="ACIYSB_002267"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGC5429313.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(18854..19840)
                     /gene="dcyD"
                     /locus_tag="ACIYSB_002268"
     CDS             complement(18854..19840)
                     /gene="dcyD"
                     /locus_tag="ACIYSB_002268"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGC5429314.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPCFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(19945..20745)
                     /gene="tcyJ"
                     /locus_tag="ACIYSB_002269"
     CDS             complement(19945..20745)
                     /gene="tcyJ"
                     /locus_tag="ACIYSB_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGC5429315.1"
                     /translation="MKLAHLGRQALMGVMVVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(20833..21384)
                     /gene="fliZ"
                     /locus_tag="ACIYSB_002270"
     CDS             complement(20833..21384)
                     /gene="fliZ"
                     /locus_tag="ACIYSB_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGC5429316.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLEQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21430..22149)
                     /gene="fliA"
                     /locus_tag="ACIYSB_002271"
     CDS             complement(21430..22149)
                     /gene="fliA"
                     /locus_tag="ACIYSB_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGC5429317.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22313..23362)
                     /locus_tag="ACIYSB_002272"
     CDS             complement(22313..23362)
                     /locus_tag="ACIYSB_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HGC5429318.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTAEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKNDSMKIQIGANDNQTISIGL
                     QQIDSTTLNLKGFTVSGMADFSAAKLTAADDTAIAAADVKDAGGKQVNLLSYTDTASN
                     STKYAVVDSATGKYMEATVVITGTAAAVTVGAAEVARAATADPLKALDAAIAKVDKFR
                     SSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLS
                     KANQVPQQVLSLLQG"
     gene            23610..25025
                     /gene="fliD"
                     /locus_tag="ACIYSB_002273"
     CDS             23610..25025
                     /gene="fliD"
                     /locus_tag="ACIYSB_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGC5429319.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SNNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            25041..25451
                     /gene="fliS"
                     /locus_tag="ACIYSB_002274"
     CDS             25041..25451
                     /gene="fliS"
                     /locus_tag="ACIYSB_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGC5429320.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            25451..25816
                     /gene="fliT"
                     /locus_tag="ACIYSB_002275"
     CDS             25451..25816
                     /gene="fliT"
                     /locus_tag="ACIYSB_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGC5429321.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            25894..27381
                     /gene="amyA"
                     /locus_tag="ACIYSB_002276"
     CDS             25894..27381
                     /gene="amyA"
                     /locus_tag="ACIYSB_002276"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGC5429322.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(27415..27828)
                     /gene="yedD"
                     /locus_tag="ACIYSB_002277"
     CDS             complement(27415..27828)
                     /gene="yedD"
                     /locus_tag="ACIYSB_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGC5429323.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            28015..29220
                     /gene="yedE"
                     /locus_tag="ACIYSB_002278"
     CDS             28015..29220
                     /gene="yedE"
                     /locus_tag="ACIYSB_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGC5429324.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGLLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            29217..29450
                     /gene="yedF"
                     /locus_tag="ACIYSB_002279"
     CDS             29217..29450
                     /gene="yedF"
                     /locus_tag="ACIYSB_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGC5429325.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            29559..29981
                     /locus_tag="ACIYSB_002280"
                     /pseudo
     CDS             29559..29981
                     /locus_tag="ACIYSB_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
     gene            complement(29983..30267)
                     /locus_tag="ACIYSB_002281"
                     /pseudo
     CDS             complement(29983..30267)
                     /locus_tag="ACIYSB_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            30290..30814
                     /gene="tnpA"
                     /locus_tag="ACIYSB_002282"
     CDS             30290..30814
                     /gene="tnpA"
                     /locus_tag="ACIYSB_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HGC5429326.1"
                     /translation="MKKETDIRRGRHCVFLKHVHLVFVTKYRRQIFDHDATEKLRTYF
                     SNVCADFEAERVEMDGGPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRPDIAVR
                     YYDKGVLWSPGYFVSSCILTYQLDLITTISVPNRISNVISIPLIIPSTSCNFPTLELH
                     IVQTPDAKKSHVHF"
     gene            complement(30971..31768)
                     /gene="ybcM"
                     /locus_tag="ACIYSB_002283"
     CDS             complement(30971..31768)
                     /gene="ybcM"
                     /locus_tag="ACIYSB_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5429327.1"
                     /translation="MLAKDKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(31778..32329)
                     /gene="ybcL"
                     /locus_tag="ACIYSB_002284"
     CDS             complement(31778..32329)
                     /gene="ybcL"
                     /locus_tag="ACIYSB_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HGC5429328.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(32498..32830)
                     /gene="emrE"
                     /locus_tag="ACIYSB_002285"
     CDS             complement(32498..32830)
                     /gene="emrE"
                     /locus_tag="ACIYSB_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HGC5429329.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(33174..33488)
                     /gene="fliE"
                     /locus_tag="ACIYSB_002286"
     CDS             complement(33174..33488)
                     /gene="fliE"
                     /locus_tag="ACIYSB_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGC5429330.1"
                     /translation="MSAIQGIEGVISQLQTTAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            33703..35355
                     /gene="fliF"
                     /locus_tag="ACIYSB_002287"
     CDS             33703..35355
                     /gene="fliF"
                     /locus_tag="ACIYSB_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGC5429331.1"
                     /translation="MNAAAAQTKSLEWLNRLRANPKIPLIVTGSAAVAVMVALILWAK
                     TPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLAQQGLPK
                     GGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPSLF
                     VREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQS
                     NTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQ
                     YRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNNRQ
                     QQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPL
                     PLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQAFIDQLL
                     AAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQALAREEVEDAVEVRLSKDEQL
                     QQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            35348..36343
                     /gene="fliG"
                     /locus_tag="ACIYSB_002288"
     CDS             35348..36343
                     /gene="fliG"
                     /locus_tag="ACIYSB_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGC5429332.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            36336..37022
                     /gene="fliH"
                     /locus_tag="ACIYSB_002289"
     CDS             36336..37022
                     /gene="fliH"
                     /locus_tag="ACIYSB_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGC5429333.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            37022..38395
                     /gene="fliI"
                     /locus_tag="ACIYSB_002290"
     CDS             37022..38395
                     /gene="fliI"
                     /locus_tag="ACIYSB_002290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGC5429334.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAITALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            38414..38857
                     /gene="fliJ"
                     /locus_tag="ACIYSB_002291"
     CDS             38414..38857
                     /gene="fliJ"
                     /locus_tag="ACIYSB_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGC5429335.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            38854..39981
                     /gene="fliK"
                     /locus_tag="ACIYSB_002292"
     CDS             38854..39981
                     /gene="fliK"
                     /locus_tag="ACIYSB_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGC5429336.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALTGEATTD
                     KATPQLLVATDKPTAKGEPLVSDILSDAQQADLLIPVDETPPVINDEQSTLTPLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDGPGTPAQPLTALVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLQPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            40086..40550
                     /gene="fliL"
                     /locus_tag="ACIYSB_002293"
     CDS             40086..40550
                     /gene="fliL"
                     /locus_tag="ACIYSB_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGC5429337.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            40555..41559
                     /gene="fliM"
                     /locus_tag="ACIYSB_002294"
     CDS             40555..41559
                     /gene="fliM"
                     /locus_tag="ACIYSB_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGC5429338.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            41556..41969
                     /gene="fliN"
                     /locus_tag="ACIYSB_002295"
     CDS             41556..41969
                     /gene="fliN"
                     /locus_tag="ACIYSB_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGC5429339.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            41972..42337
                     /gene="fliO"
                     /locus_tag="ACIYSB_002296"
     CDS             41972..42337
                     /gene="fliO"
                     /locus_tag="ACIYSB_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGC5429340.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            42337..43074
                     /gene="fliP"
                     /locus_tag="ACIYSB_002297"
     CDS             42337..43074
                     /gene="fliP"
                     /locus_tag="ACIYSB_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGC5429341.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            43084..43353
                     /gene="fliQ"
                     /locus_tag="ACIYSB_002298"
     CDS             43084..43353
                     /gene="fliQ"
                     /locus_tag="ACIYSB_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGC5429342.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            43362..44147
                     /gene="fliR"
                     /locus_tag="ACIYSB_002299"
     CDS             43362..44147
                     /gene="fliR"
                     /locus_tag="ACIYSB_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGC5429343.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            44437..45060
                     /gene="rcsA"
                     /locus_tag="ACIYSB_002300"
     CDS             44437..45060
                     /gene="rcsA"
                     /locus_tag="ACIYSB_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGC5429344.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(45104..45292)
                     /gene="dsrB"
                     /locus_tag="ACIYSB_002301"
     CDS             complement(45104..45292)
                     /gene="dsrB"
                     /locus_tag="ACIYSB_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGC5429345.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            45455..45682
                     /gene="yodD"
                     /locus_tag="ACIYSB_002302"
     CDS             45455..45682
                     /gene="yodD"
                     /locus_tag="ACIYSB_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGC5429346.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            45980..46795
                     /gene="yedP"
                     /locus_tag="ACIYSB_002303"
     CDS             45980..46795
                     /gene="yedP"
                     /locus_tag="ACIYSB_002303"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGC5429347.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(46792..48486)
                     /gene="dgcQ"
                     /locus_tag="ACIYSB_002304"
     CDS             complement(46792..48486)
                     /gene="dgcQ"
                     /locus_tag="ACIYSB_002304"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGC5429348.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGCIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(48682..48864)
                     /gene="yodC"
                     /locus_tag="ACIYSB_002305"
     CDS             complement(48682..48864)
                     /gene="yodC"
                     /locus_tag="ACIYSB_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGC5429349.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(48943..49860)
                     /gene="yedI"
                     /locus_tag="ACIYSB_002306"
     CDS             complement(48943..49860)
                     /gene="yedI"
                     /locus_tag="ACIYSB_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGC5429350.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            50033..50953
                     /gene="yedA"
                     /locus_tag="ACIYSB_002307"
     CDS             50033..50953
                     /gene="yedA"
                     /locus_tag="ACIYSB_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGC5429351.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLTGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(50942..51412)
                     /gene="vsr"
                     /locus_tag="ACIYSB_002308"
     CDS             complement(50942..51412)
                     /gene="vsr"
                     /locus_tag="ACIYSB_002308"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGC5429352.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(51393..52811)
                     /gene="dcm"
                     /locus_tag="ACIYSB_002309"
     CDS             complement(51393..52811)
                     /gene="dcm"
                     /locus_tag="ACIYSB_002309"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGC5429353.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQVRGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(52878..53315)
                     /locus_tag="ACIYSB_002310"
                     /pseudo
     CDS             complement(52878..53315)
                     /locus_tag="ACIYSB_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
BASE COUNT        13249 a        13754 c        13064 g        13341 t
ORIGIN      
        1 tactaataat cagactaata tttacctgtt tgaccgagtt gggattgcgt cgtttctcca
       61 ttaggagtaa agctttaatg tcacctgaag ttcacagaat aaggaacagg ttatgagcta
      121 tagcaatatt cttgtcgctg ttgcggttac accggaaagt cagcaactgc tggcaaaagc
      181 ggtatctatc gccaggccag taaagggaca catcagttta attactctcg cttccgaccc
      241 ggaaatgtac aatcaattag ctgcgccgat gcttgaagat ttacgcagcg tgatgcagga
      301 agaaacgcaa agctttcttg ataagttaat tcaggatgcg gggtatcccg ttgacaagac
      361 gtttattgcc tacggtgagt taagcgaaca tattctggaa gtatgtcaca agcatcattt
      421 cgatttggtg atttgcggta atcacaatca cagtttcttt tcgcgagcat cctgctcggc
      481 gaaaagagtt attgcctcaa gtgaggtcga tgtgctgtta gttccactta cgggagatta
      541 accgctccta cgcaagcttt ggaaaggtag caactttatc gcgctgctgg ctttccgcgc
      601 ttcgcggaac tatctgcttt aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt
      661 ttttcacgat aacgtccagc atttctgcat gacgggaaat acgttccgcc agcgacatag
      721 tcaatgcacg atccagcgca gctgcaactt cgtcacgatc gtaggggtta acaattaacg
      781 ccgacgttaa ctcgtttgcc gctcccgcaa attgcgaaag aacaagaacg cccggattgg
      841 ctgggtcctg agcagcaaca tactcttttg ctaccaggtt catcccgtca cgcagtggcg
      901 tcactaagcc cacgtcagag tagcggaata ttttcatcag taatttacgg tcaaaatgct
      961 gattcaaata ataaagcggc gtccagccta attgcccgta tttaccatta attcgtccag
     1021 cttcattttc gagctgatga cgaatatcct gataggcttg cacatcacca cgcgacgttg
     1081 gtgcaatctg ggtataacga attttaccat gatgctgcgg atatttttcc agcaacgctt
     1141 cataggcgag aaaacgctct ggcaaacctt tggaataatc cagccgttcg acagaaaaga
     1201 tattttgtac gtttttcagt tccgctttaa gttgcgccag ttttggcggc agtggcccgg
     1261 cagcctgttt ggctatttct ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg
     1321 ctttgcccca ggctgtatgg ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa
     1381 gacaatccag gaacgccaga cgatcgtttt ctgtctggaa acccagcaaa tcataatcac
     1441 aaagctgttc aagcaaggtg tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga
     1501 aaggaatatg cagaaagaaa ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg
     1561 caaatggcaa caggtgataa tcgtggatcc agataatgtc atcgtcttgc aacagcggca
     1621 gtaatttatc tgccagcaac gcatttacgc gtagatagcc gtcccaggca ggacgctgaa
     1681 attgcaccag atcgagccga taatgaaaag cgggccagag aacggcattg gagaattggt
     1741 tgtagtattc gtcaaggtcc tgttcgctga ggttaaaaga ggcccacgta atgttacctt
     1801 ttttcacctt ttttagcggc tgatcctcat tccctgtttc accactccag ccaaaccaca
     1861 gtccgcctgc ggctttcagt gcccccagta tgccaacggc aaggccaccg gcactggcgg
     1921 cgtgctcgtc tggtggtgca atccggttag atactacgac taaacgactc atagtcatca
     1981 ctcctgttat tttctctttt ttgttgtaat gcggtggtta tcatttcaag ccagctccag
     2041 acatccggca cacccgccag tcgccatgat gcctgagttg cacctgtgcc aatttttact
     2101 gacattccgc ccagtcggtt aacgactgcg aagccagatt catcggttaa atcatcgccc
     2161 agaaatacgg gcgttcgccc gataaaggga gcttcctgca taaaagctgc aattgcctca
     2221 cctttactgg tacctctcgg tttgatctcg acaacacact ttccctgctg taacgccatt
     2281 tgtggccaga tctgagtaat acgttgcgct aatgtcatta atgcgtcttc atgctgcgga
     2341 gcctgacgat aatgcagcgc aaaagccatc cctttcgcct ccagctccgc gccgggatac
     2401 tgagcgatga ctgtatgcag ttgcacgcta atatcacgcg caatcgcatc cggcagatga
     2461 acgatatgtg ttttaccatt gatgtcacgg cgctccgccc catgcacgcc cgctaacggg
     2521 aagcgataag gttttgccag tgcgtcaagc tccaccattg agcgccctga tatcaatgcc
     2581 aatgcaccat cacttgcggt tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg
     2641 acgacctgat cgggatgcgg tttgatttcc gccagcgttc catcaagatc aaaaaaccag
     2701 gcatatttcg cggatagttc aggggtttcg gttaacggtt ctgtcacccg gttctcctcc
     2761 ttctcttttc ttattgtgtt gcgcagaacg cgctttacag acatgtaagt atagacagtg
     2821 tgacgggggt cgccatttgc aacgaagaac agccaaccgt tatggcggtt ggctatggtg
     2881 ggaaaaaacg ctaaattgtt gcagaaaaaa gcatcagaca gtgcgtttcg ctttttgctt
     2941 gtaacggtcg aagatcaccg ctgccagcag gattaagccg cgaaccacgt actgcgcgaa
     3001 aggagaaata ttaagcaggt tcatggcgtt ttccacggtg cctaaaatta agatacccgc
     3061 caccacatat gagatttttc cgatgccacc tttcagagaa acgccaccta aaacgcaggc
     3121 ggagataaca atcagctcat aaccaatcga cgtcattggc tgcccactgg tcatacgtga
     3181 agccagaata attccggcta tcgctgatac caggcctgag agaacaaaga taataatttt
     3241 ggtgcgaaca accggtacac ccgccagacg cgcggcctct tcgttcccgc caatcgccag
     3301 ggtgttacga ccaaaggtgg ttttattcag cagcaaacca aagataatca gacacgcgac
     3361 ggtgagccag attggcgcag gcagaccgaa ccagttggcg taaccaaggg caaagaagct
     3421 ttcatcttcg ataccgaccg ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat
     3481 ctgcatcgtt gccaatgtcg tgatcagagc atttattttc agtttggcga taacaaagcc
     3541 attgaccagg ccacagagaa cgcccagcaa caaccccgct gccacgccaa tccacaggct
     3601 ttcagtcagg ttaataacca ccgccgtggt gacacccgca caggcaatta cggaggcgac
     3661 agaaaggtca aagtcaccgg aagcgaggca gaacaacatg ccacaagcca ccatccccga
     3721 catggaaatt gccaggccca accctttcat attaatgaag gtggcaaaat ttgggacaaa
     3781 aatggcacag gcgataaaga gcaccgcaaa caccaccagc atgccgtact gatcccagat
     3841 acgcccgaag ctgaatgacg acttaggtgc gccagacccc gatgtagaaa cagaagacat
     3901 catactctcc ttactcaggc aacagcctgg ctgactttag gcatcgcaag gctcagtgcc
     3961 tgacgctcat ctgcctgctc gtgtaacaat tcaccggcga tttcaccttc ccgcatcacc
     4021 acaatccggt cggcaacgcc gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc
     4081 acgccctgcg ccgccagcgc ataaattacg ttatatattt cgtgcttagc gccaacatca
     4141 atgccgcgcg taggttcatc cagcaaaatg accttcatct cttccgataa ccagcggccc
     4201 agaatggctt tttgctgatt tccgcctgag agattcatga tcagttgctc cgcgcccggc
     4261 gttttgatgt tgagcgaacg aatgtggtga tcggcattgt tttcttccca accgttgttg
     4321 attacacaac cgccgagcac atgtttacgt ctggcactga tgttgatatt gtcgcgaacg
     4381 gagtgcacgg gaataatgcc ttccgctttg cgatcttccg ggcagagcat catgcctgcg
     4441 gcaatggcgt ggctcggttt acgaatatcg atcggctgtt ggtcgatata aacctgaccg
     4501 gcggtgattt gcgtcccgcc aaacatgcct ttcattaatt cgctacgccc cgcccctacc
     4561 agaccaaaca gcccaacaat ttcaccactg cgaaccgcca gacttattgg cgtacgcacg
     4621 cctggtgctt tcacagcatc aagacgtagg cgctcctcgc cataactacg cggttgccag
     4681 ccgtagatat cgccaatgtc gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc
     4741 tgctgcatat cggtaaaggt tttgacataa cgtccatctt taaagacagt aatggcatcg
     4801 ctgagggcaa atatttcttc catacggtga gaaacgtata agattacccg cccctctttt
     4861 cgcagttcac gaataacgcg gaaaagattg tcgatttcac gggcagagag ggagctggtt
     4921 ggctcatcaa aggcgataat tttggcgtta cgcgccagcg ctttggcgat ttcaaccatc
     4981 tgccactgac caatggagag atatttcagc ggcgtgtccg ggtcaatatc cataccaaga
     5041 tgtttaagtt gtaaacccgc ctcataattc agcaatgagc gattcacaat gccgccttta
     5101 tgcggcagct ggccgagata gatgttttcc gcgacggtca tttccggcac gagatgcagt
     5161 tcctggtaaa taatcgccac gcccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt
     5221 tcctgcccat taatcactac agaacccgtg gttggcgcat agttgccgct gaggattttt
     5281 aagagagttg attttcctgc gccattttca cccatcaacg catgaacctg accggcatag
     5341 cagtcaaaac taatatccgt cagcgcctta acgccgggaa acgttttacc gatgccgcga
     5401 aatgagagat acggggtaga ctgttgcata acgtctccgt gaatcactgg tcgttcacac
     5461 cccctctgca tcatgcagag gggaattttt ccggcaaatt acttaccgcc taaacctttt
     5521 ttctccagtt cttctttaaa gttgtcacgc gtgatcagta ccacgtcggt aacttcggta
     5581 aattttggcg gttcaacgtc ttttgctacc cagttgtaaa gcatttcgct ggatttatag
     5641 ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc cggttgcctg tgctttagac
     5701 agttcgctca ccgcatccac accgttaatg ccaatgccga tgatatcggc cgctttaaag
     5761 ccctgacctt ccgtcgcgcg tacgccgccc agcacggtgc tgtcgttcat accgacgatc
     5821 agccaatgtt taacttccgg atgttgaacc agcattgagt tggcagcgtc aaatgccccc
     5881 gggatgtcgt tagatttggt aggtacctga taaatttgtt tttccgggaa tccggccgct
     5941 ttcagcgcat ccatagatcc cgtagtacgg cggcgggcgg tatccagttc gttggcggta
     6001 atcgccatca ccgcgctttc tttgacatcc cagccacgtt tctgcatctc tttatacagt
     6061 tcctggccct gacgttcgcc aattttagtc gccgccatca tcaccagcgg aacggtatcc
     6121 attggcttac ctttggcgtt aacaaactgg tcatccacgg caatgacttt catatcgtag
     6181 ccacgcgctt tcgcgacgat ggcagagccg agtttggggt ccggagtaca aataacgaaa
     6241 ccttttgcgc cactggcagc caggctgtcg atcgcgttca atgttttttc gccatccggc
     6301 acggcaatct taataacctc aaaccctaaa tccttcccgg ctttatcggc aaacttccat
     6361 tcggtctgga accacggctc ttccggttgc ttcaccagaa aaccgagctt caggttctcc
     6421 gccatagcgg attgtgacat aacggctgcc agaccaatgg ctgccagggc tttagtaaat
     6481 ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta gggcaaaaac gaatgacatt
     6541 cgttaaatta atcggaaaac aaagcattac cttttaacta aaagataagt gactgtgttg
     6601 acatagtttt agcgagaaat taattctcca taggagagca atatcacatc gcagaattac
     6661 agtgagaacg tgcataaatt tagcgggaaa agacataagg gaaagccaat ttgtcagaca
     6721 aattgtcgaa tgcacagcag attaatccat aagattagcc tggaaatcct tgttgtcttt
     6781 ggtacccatg cgggatgtct tctttttaac cagtcaatag gccgcattac ctggcgttga
     6841 gtttttgaaa tggtgtaata accgcaactc aaagatgtgg aaaatgcacg tcattcattt
     6901 cgtcattaat tatcactgtg ctcattaatt aacagaacac gtataatgag agccatctcg
     6961 caaaaatgaa aaaacgtttt ataaaatcat cacttcatca tgaattcaaa ttcattgatt
     7021 aatatcaaca agatacaaaa agcactatca ttaaaattca ttgcagttac attgatttca
     7081 tcaatgaaat gtaaaaatat ataaacttga tgatttaagc attttcttat acccgttcag
     7141 acgttattct tatttcagat catcgtcaga attgactcca cgatcacatt tcggaccggc
     7201 agaaaggaat tattctgcaa acagtaatta tggtgttttg atttatcttg cacctctcca
     7261 cttctggata taaggatatt aggtatggca accgctggaa tgcttctcaa actcaactct
     7321 caaatgaacc gcgagtttta cgcatccaat ctctaccttc acctgagtaa ctggtgttct
     7381 gaacagagtc tgaacggcac cgccactttc cttcgcgccc aggcacagag taatgtgacc
     7441 caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg ctacccccat cgttaaagcc
     7501 attgatgttc ccggtgaaaa actgaactct ctggaagaac tgttccaaaa aacgatggaa
     7561 gaatacgagc aacgttctag tacgttggca cagttagccg atgaagcgaa agaactgaat
     7621 gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag aacagcagca tgatggtctg
     7681 ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac ttgcgggtat gtgccctgtg
     7741 cagaccgacc aacatgttct gaatgtcgtg tcacaccagc tgcattgatc atcatcggcg
     7801 ctaatgcatt gcgccgatga aggttttgag aaaccgctgc ctcatctgtt tgaagcagcg
     7861 gtttttttaa tgggattcac cctgtggggt aaacttgagt tcaataagcg cgatggcttt
     7921 ttggattgcc cgcatggtga ccgggtctgc ggcggcggga tggttagtaa agtcgatatt
     7981 cttcagctga ctggacattt tttcacgaac ttcaacgggc gcgattacat cgagaacatc
     8041 cagaatttgt ttgataacca actggcaagc aaccacatca gaaaccaatt cctgatcggc
     8101 attcagcggc tgggacatcg taaactcctg atagcatttt gaaagccgtt atagtagcga
     8161 cttcacatct tcagcgatag tcacatccac cgtcatcagg acacaaaaaa acctgccgga
     8221 gcaggttttt tgttatcgga acatattgcc tggcggtacg tctttgaacg tcttgcaata
     8281 gttattgaac atacttttca ggattttgcg cagtttcatc gcggcactcc gaccatttgt
     8341 tatacaggtg ttattgtctt tgcgcttata atatgacaac catcacaaaa atcaatcttt
     8401 atgtgataca aatcacataa ataccccttt aatgttataa aaatgataat caaaaaacag
     8461 cccccctatt tctgacacct acagatggca agaaatagcg cctgccaggc gtcttttccg
     8521 gccattgtcg cagcactgta acgcgtaaaa tagtgctttc tcttactctt ctggctggac
     8581 catgagactt ctgattctga ctctttcatt aataacgctg gcggggtgta cggttactcg
     8641 ccaagcccat gtgagcgaag ttgacgcggc aacaggtatt gtacggttag tttatgacca
     8701 ggcttttttg cagcatgccc ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc
     8761 atgccagcag gaaggctata cccacgcggt cccctttggt cagccagtag gcaactgtag
     8821 cctttttgcg ggttccttat gtttgaatac ggaattcact ttgtcatatc agtgccatca
     8881 ttccgccttc cctgtctttc tctaaatatt ttaatcagcg aggggatctt cgctgattaa
     8941 agaaatagcg gtaatgcgtt ttattcccat tcgtattttt aataattgaa gtttatattt
     9001 taccttttgc aaataataaa ataacaaatt atagtgacgc cacagcaaca aatataacct
     9061 ttgtggagca ctatcatgct gaaaccagaa atgattgaaa aacttaatga gcagatgaac
     9121 ctggaactgt actcttcact gctttatcag caaatgagcg cctggtgcag ctatcatacc
     9181 ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg aagagatgac gcatatgcag
     9241 cgtctgtttg attacctgac tgataccggc aatttaccgc gtattaatac cgttgaatct
     9301 ccgtttgctg aatattcctc acttgatgaa ttattccagg aaacctataa acacgaacaa
     9361 ttaatcaccc agaaaattaa cgaactggct catgctgcaa tgaccaatca ggactaccca
     9421 acatttaatt tcctgcaatg gtatgtttct gagcagcatg aagaagagaa actgttcaaa
     9481 tcgattattg ataaattaag cctggcaggc aaaagcggcg aaggtctgta ttttatcgac
     9541 aaagaactct ctaccctcga cacacaaaac taatgctctc cggcggcagg tttactgccg
     9601 ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt cgaaaaaccg tctttcgaag
     9661 gtataaattt gccttttgct gccagaaacg ctaccagctc tcccgctgtc atcccttctg
     9721 ccgagcaggt gtgaaaacgt gcctgttcac caaaacgcgc tttaatcgca gcttccagac
     9781 tggcatgcgt atattgctcg cctgattcaa tcatcatatt taacacttca tgaccgtgaa
     9841 tagagtccat cgtccctcct caaaaaaagc ctagcgtagc gattgccgct tatgaagact
     9901 ttgcgccagc gcaggactga atgcttttta ttgtacattt atatttacac catatgtaac
     9961 gtcggtttga cgaagcagcc gttatgcctt aacctgcgcc gcagatatca ctcataaaga
    10021 tcgtcaggac agaagaaagc gtgaaaaaca gaaccctggg aagtgttttt atcgtggcgg
    10081 gaaccacaat tggcgcaggc atgctggcaa tgccgctggc tgcggccggt gttggtttta
    10141 gcgttacgtt aatcttgttg attgggcttt gggcgttgat gtgctacacg gcgctattac
    10201 tgctggaggt gtaccagcat gttccggcag ataccggtct gggcacgctg gcaaaacgct
    10261 atctgggacg ctacggtcaa tggctgacgg gcttcagtat gatgttctta atgtatgctc
    10321 tgactgcggc atacatcagc ggtgccggtg aattgttggc ctccagcatc agcgactgga
    10381 caggtatttc tatgtcggca accgctggcg tgctgttgtt cacttttgtt gccggtggcg
    10441 tggtttgtgt cggaacttca ctggtcgatt tatttaaccg ttttctgttc agcgccaaga
    10501 ttatttttct ggtggtaatg ctggtactac tgctgccgca tattcacaaa gtgaatcttt
    10561 taaccctgcc gttgcaacag gggctggttc tgtctgcaat cccggtgatt tttacgtcgt
    10621 ttggttttca cggtagcgtg ccgagtattg tcagctatat ggatggcaac attcgtaagc
    10681 tacgctgggt gtttataatc ggtagtgcga tccccctggt ggcatatatt ttctggcagg
    10741 tggcgacgct tggcagcatt gattcaacaa cctttatggg attgctggct aatcatgctg
    10801 gattaaacgg gctgttacag gcgttacgcg aaatggtggc ctctccgcat gttgagctgg
    10861 cagtgcattt atttgctgat ttagccctcg ccacgtcatt tctcggcgtt gcgttaggct
    10921 tatttgatta tctggctgat ttatttcagc gttcaaatac cgttggtgga cggttgcaaa
    10981 ctggtgcaat tacgtttctg ccgccgttgg cgtttgcact gttttatcca cgaggatttg
    11041 tgatggcgct gggttacgcc ggtgtggcgc tggcggtact ggcattgatt atcccttcgc
    11101 tgttgacctg gcaaagcaga aagcacaatc ctcaggcggg ttaccgggtc aaaggtggtc
    11161 gtccggcgct ggtggtggtg tttctctgtg gtattgctgt gattggcgtg caatttttga
    11221 ttgcggcagg gttgttacca gaagtggggt gatcagatag cctcaaattc cttattgggt
    11281 gccagaatta acgctgacac ccaatttggc ctcttaatgc aggcagcact gcttaaattt
    11341 cttaccacta ccgcacgggc aaggatcgtt acgccccggt ttctcttctg ctttgatcgg
    11401 ttgctgaaca gctttttcct gcggatgcgc catccagtac gcatgtagat caagcgccgc
    11461 cagtcgaatg gcatctacgc tctcttcaaa cgcttctggc gacatctttt ctacccgctc
    11521 gaagttttcc tcagtaccgt gcagcgcaat cgcctccagc gctggtttta acgaatcggg
    11581 caacgttgac cagtcagaaa gtgccacgcc ccgcatatag ccaaagcacc actcctcaac
    11641 aatcgtcagc tcgctgccat caacttctcg caagccgaat aacggctcaa actgctccgg
    11701 gaattcgttc agacgctctg cggtatcggc catatgttga aaagccagat tcataaagcg
    11761 cgtcatctct ttctctgacg cccagcgcgg cacatagtca gccccacccc acacggcaac
    11821 cagccactgt tccggttcaa tctcttgcgg agaactcaac accgccgtca ataaaccgtc
    11881 cagctccgcc acatcaagga tggcgtggtc agtgttgtat ttggtcagaa tatcgtccag
    11941 ccattccaac tcactttcgt ttaacggtcc cgttttcata cgcttttcct tgtggatctc
    12001 aactcgccag cacctatctt acatgccggt ccgtatcaga gatacttttt gagtggcttt
    12061 gctggtgatt aaaaattaag gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc
    12121 gaaggtggtt tcacgacact gcttattgct ttgattttat tcttatcttt cccatggtac
    12181 ccggagcggg acttgaaccc gcacagcgcg aacgccgagg gattttaaat cccttgtgtc
    12241 taccgattcc accatccggg ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg
    12301 actagacgga tttgcaatcc gctacataac cgctttgtta acgcgccaaa ttcttcaggc
    12361 ctttcagcca gacatccgct tgaggccgat gtcttttaaa ctggagcggg aaacgagact
    12421 cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcatt
    12481 catcaagcaa tcagttaatc acttgatttt attatcgtct ggcaatcagc gccgccgttc
    12541 gatgcgttgc attctactta cctggcgcga tgagtcaacg atatttttca ccacttttga
    12601 tcgtttgctg aaaattgcac cgaaacgatc actgatcaag caaatctgca cgcgcagcgc
    12661 tcaaatattg caacattgac cacagagtca gtaccgcagc cacaaagaaa agtgcaatac
    12721 cggcgtactc aacccaaatg ttcggacgcc acagcagcca ggccaacgcc accatctggg
    12781 ccgtagtttt cactttccca atccaggaga cagccacgct actgcgttta cccaactccg
    12841 ccatccattc gcgtagcgca gaaataataa tttcacgggc gatcatcgtt gccgccggta
    12901 aggtcaccca ccagctgtga taatgctcgg ttaccagcac catggcgata gccacgagaa
    12961 ctttatctgc gacagggtca aggaaagcac caaaccgggt actctggttc cagcggcgtg
    13021 ccagaaaacc atcgaaccag tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa
    13081 acggcgacca ggtgacaggc agataaaaga ccaatacaaa gaatgggata aggatgacac
    13141 ggaacagtgt aagcaacgta gggatattaa attgcataat gacgggtaac tatctgttgt
    13201 cagtaagatt acccctatgt tgctacagag acatcaatgt ttcaacgacc agaagatctt
    13261 ttctgccaga ccttgcgaaa tacccggcac ttttgcaatt tcctcgacgc tggcgttacg
    13321 taaaccttgc aaaccgccca tatatttcaa caacatttgc cgacgttttg gcccgacgcc
    13381 ttcaatggtt tccagggaac tggtattttt gaccttcgcc cgttttttac ggtgcccgcc
    13441 aatcgcgtga tcatgtgatt catcgcgaat atgctggata acatgcagcg cgggtgaatc
    13501 tggcggcaaa ctaaatccct caccttccgg ctcaaagaac agcgtttcca gtccagcctt
    13561 acgatctgct cctttggcaa cgccaagtag cagcggatga tttttatccc atgagacatc
    13621 cagttcggcg aagacatttt tcgcctgcgc aagctggcct ttgccgccgt cgataaggat
    13681 cacatccggg atcttactgt cgtcaatggc tttaccataa cgccgacgca gcacctgatt
    13741 catcgccgca taatcatcgc ccggcgtgat gcctgtaatg ttatagcgcc gatactccgc
    13801 acgcagcggg ccgttagcat caaacaccac acaggaagcg acggtttgtt cgcccatggt
    13861 atggctgatg tcaaagcact ccatccgctt cacttccggc aatttcaaca cgctggcaag
    13921 cgcggtcagt cgctggtgaa cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc
    13981 cgcattggtg cgcgcgagtt tcagataacg cgccctatcg ccgcgaggtt tggtttgaac
    14041 attaatcttg cgtcccgcca gttctgaaag ggaatcggcg agcagcgttt tatcgctaag
    14101 attaaaatcg agcaggatct cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa
    14161 ctggcctacg aaggtttcta ccacctcgct cagttccgta ccgccaggca ctttcgggaa
    14221 atagctgcgg ctgccgagca ctttgccctg acgaatgaac aatacgtgga cacaagccat
    14281 gcccgcatcg aacgccacac caataacgtc gaggtcgtcg ccggtattgg aaacgaattg
    14341 tttttcggtg acgcgtcgca ccgcctgaat ttggtcgcga atacgtgcag cttcttcaaa
    14401 ctccagattc tggctggcag tttccatacg actaatgagt tgcgtaagca cctgatcatc
    14461 tttgccagac aaaaacaggc gcacatactc gacctgctga gcgtattctt cttcactcac
    14521 cagtccttca acgcacggtc ccagacagcg ccctatctgg tattgcagac acggacgcga
    14581 gcgattgcga taaacactat tttcgcactg gcgaatgggg aaaatctttt gcagtagcgc
    14641 cagtgtttca cgtacggcat agccattcgg gaacgggccg aaatattcac ctttggcatg
    14701 cttcgcacca cgatgcatcg ccagacgcgg gtgggtatca ccactcagga agataaaagg
    14761 atatgattta tcatcgcgta gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt
    14821 gtgttccagc aacagcgctt cggtttctgt gtgagtaacc gttacatcaa tttgctggat
    14881 ctgggcgacc agcgcttcgg ttttgcgcga agcgaggttg ctacggaaat agctggaaag
    14941 ccgttttttc aggtctttcg ctttgccgac atagataacc gtaccaccag catcgtacat
    15001 gcgataaacg cctggctggc tggttacggt ttttaaaaac gcttttgcgt caaactgatc
    15061 actcactgac ttgataatgt ctccgcatta cacagaccat ggcgaattgc caggtgagtc
    15121 agctcaacat cgccatgaat gtttagttta ctgaacatac gatagcggta gctgttcacc
    15181 gttttcggac tgagattgag ctgttctgag atctcattga ccttctggcc cttggtgatc
    15241 atcagcataa tctgcaattc acgttcagac aaactggcaa atgggctttc tgttttttct
    15301 ggttcgatct ggcttaacgc catttgttga gcgatgtcag aagcaatgta acgctgccct
    15361 gaatagacag aacgaatcgc actcacgact tcctgcggag ccgcgccttt gctgaggtag
    15421 cccgcagcac cggcctgcat gactttcgct ggtaaagggt tttctgtatg gacggtaagc
    15481 atgatgattt tgacatcagc tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca
    15541 atgcccggca tactcatgtc cattagcacc acgtcaacgg catttgtccg gcaccactta
    15601 acggcgtctt caccgcacga tgcctcaccg acgactttta taccctttat atcttccaga
    15661 atgcgtcgta tccctgcgcg caccagttcg tggtcatcaa caagtagaac gttgatcaaa
    15721 ggaatatctc cagaaatagg gataacgcta ctgatagtta gtcattcgta tattagcggt
    15781 ttttattgca actttgaaac gttaaaaatg gtgggtattc gatttttctc tcgtttttgt
    15841 caattcgatt gtccacagtt atggtcacgc ccgtcaccaa cggcttaccg gatcgtttca
    15901 cagtaagccc tgcgacgaaa tgtgacaaaa attaccttta ttcagcaaaa atgaaaatca
    15961 gcccgctgaa tatgtaacat taattaaccc gaacaatacg ggcaaaaaac attcatgata
    16021 aaaatattta ttggtcatta tattaacgca tttaatagca ccgttgacga tacgctcaaa
    16081 aaacaaccac tgctattttt agcaaagctt atggtatact ccgccgcctt aacatttttc
    16141 accgcaaatt ttcattgcaa catgacgagg aaaataaatg agtacgcctg atttttctac
    16201 tgccgagaat aatcaagaac tggcaaatga agtctcctgc ctgaaagcga tgctgacgct
    16261 gatgctgcag gcgatgggac aagctgacgc gggccgcgtg atgttaaaga tggaaaaaca
    16321 gcttgcgctg atcgaagacg aaacccaggc tgcagtattt tccaaaacgg ttaagcaaat
    16381 taaacaagcc taccgtcagt aatataaaac cggctgatag cgtgcctttc agccggtttt
    16441 tgcatctggc acgcaggaca gaaaagagat caaattaagc cagtagcggc cgcgtaacag
    16501 gcaacctggg tcttattcgg tgcattgatt tttttctgca tgtttttctg atggaaattg
    16561 accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt cccttccgcc
    16621 gtccatttca aaatttcttt ttcgcgcttg ctgaaattca tctctggcgt catcactatt
    16681 tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtaactg catttttaat
    16741 tgcagttcat cactaagaat gggtatttcg cgcgcgctgc tacgggaaaa ggacaaaaag
    16801 cccagcgccc ggtttggcag cattaaatac tgagtgacac cgcggcgtaa accatgtgcg
    16861 cgcgcggctt cccataacgg ctgtgcttcg ctgaataagt catcattcca cattaaatgg
    16921 ccctgactaa agttttcagg gttcagcacc ggatcaattg cgagaaagtt ttttgcctga
    16981 taataactaa cccacgcctc agggtaattg gtgtagaaag ccactttagg tcgcgtgaat
    17041 ggtaccgggt ggcgaacaca taacgaatag taatcgtact ctagctgctg agcctgaagc
    17101 tctatttcat ggtagacctc ttctgcggcc tccatcctct gaaaacgcaa cagcatcgtg
    17161 cgacgccagc tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag
    17221 tatcatttat aatgataatt caaactatca cataaataag atttatatat aatttatatt
    17281 attcaggcaa tgaattactt ttgcaagcca tcgcattctc ttatgttatt aatgagttat
    17341 gctgatttgt taagctgttt tatcacgctt gaaatggcgt cccgccccga caggtgaatc
    17401 gtcggggctg attttttctt attattgcag cagaaacttc tcgaggaact ggcgggtgcg
    17461 aggctgctgg ggatcggcaa ataacgcttt tgctgccccc tgctcgacta tccgcccctg
    17521 gtccataaaa atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat
    17581 caccatcgtg cgcttttcct gcgccagctg acggatggtg ttcaggactt cgcccaccag
    17641 ctctggatcc agcgccgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag
    17701 cgcacgtgca atcgcaacac gttgctgttg accgccagac aaacgacgtg gatagctggt
    17761 ttctttaccg gccagcccaa ctttcgccaa cagctcgcga gcgcgcgccg tggcctcttc
    17821 tttcggttcg cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg
    17881 cggaaacaaa ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag
    17941 cgatttttgc tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatagt
    18001 tcccgcttcc ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc
    18061 actcggaccg ataattgcca ccacttcgcc aggctttacc tcaaggtcga taccgtgcag
    18121 caccgtctga ccgtgaaatt ttttcaccag gttcttaact tcaatggcac tcattttggt
    18181 tctctctcct ggcgattaag ttgattctca aaatggttct gcaacgtcga taatactgtc
    18241 gccatgatcc agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc
    18301 gaggtaatca actgcgcctg acggaacagc tccggcacct gaattgtcgc ggccagcgac
    18361 gtatctttta ccaggctgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc
    18421 tgtggcaaaa tcgcgcgacg catagtctgc catggcgtca taccaatact ggcagctgct
    18481 tcccactgac ctttatcaat agaagaaatg gcggcgcgca gcgtttcggc agcataggcg
    18541 gcagtgttga gcgacagacc aatcattgct gacggaatgg gatctaattc gataccaaac
    18601 tgcggcaggc cgtaatagat cataaacagt tgtgcgatga gcggtgtacc acgaaaaata
    18661 gagatataaa aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg
    18721 agaataaacc cgagcagtaa gccaaaaaac atgccgccga tactgagttg cagcgtatac
    18781 ccagcccctt tcagcaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg
    18841 agcgctcccg gagctaaacg tggggatgat aggcgaacag cgcaggcgca ccgccggtat
    18901 gaataaacag aatcggccct tcatctttga aacgtttctg actgataccg tcaatcagac
    18961 ccgccatcgc ttttccggta tacacaggat caagcagaat gccttccagc cgcgccagca
    19021 atttcactgc ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat
    19081 cccagagtaa aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta
    19141 ggttaaccac tttcggcaat tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt
    19201 cgctttcagg catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc
    19261 ccgatgcgac caccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca
    19321 gcgcactctc cacataaccc agcgcgccca gagcattaga accgccaacc ggaatgacat
    19381 acgggcgaaa gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat
    19441 cggtcagtgc gtcgcacatt tcaatctggg tattgaacag atccagcaac aagcgattgc
    19501 cgttggttaa atagttttct gcggttgtgc caataggatt ttccagcagc gccacgcagt
    19561 gcagaccgag tttggcagcg actgcggcag tctggcgcac atggttagac tggatcgccc
    19621 cggcagtaat cagcgtatcg gcaccttcgc gcagagcatc tgccgcgaga aattctagct
    19681 tacgtaattt attgccgccc attgccatgg gggtgacgtc atcccgtttg atgaaaattt
    19741 cccgtcctag ataatcagaa aagcacggca gatattcgag cggcgttggc gcgccgataa
    19801 actccaaccg tggaaaacgg gttaaattat gcagtggcat aacagcctcc gatgtgtgtt
    19861 gttgtgattt tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag
    19921 cgcccttttt tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga
    19981 aagcgcttgc agagtgccgt ctttttgcat ttccgcaatt gcatcattca ccgctttcag
    20041 caggtcttca tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc
    20101 ggttactgcc agcgtatcgt tggttttctt caccagatcc agcgccgcca gacgatcaac
    20161 gaggatcgca tcgatacggc ctacgcgcag atcctgatat ttggttgggt catcatcata
    20221 ggtacgcaca tcgacaccct gaacattctg ccgcagccac tcttcatagt tggtgcccag
    20281 gccgacaccc acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc
    20341 ttttttcacc agcgcctgaa taccggaaat ggtgtaaggg gtagagaaat cgtatttttt
    20401 cttgcgctcg tcagaaatgg tgacctgatt aatcaccaca tcaatgcgtt tagagtccag
    20461 cgacgccagc ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt
    20521 tgccagctgt tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa
    20581 actgaacggc ggataagtgc cttccagccc taccagcagc gtgccgcgct ctttaacttt
    20641 attaagcaga ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgccacgac
    20701 catcacaccc atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt
    20761 tgttatgtct gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca
    20821 tcttattctt atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc
    20881 ggtacacgtt tgacgctgat agtgttgata cttccgtaac gcaatgcggt aattgttggt
    20941 gctggtggtg ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgttc
    21001 gagggaaata ttttgctcgt gtagatgatt acccaaacgg cgcagacgaa cgacatattc
    21061 acgcaccgta cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat
    21121 atcgaaaaaa tcactctgtg ttttgcaatg taaatcaccg cagaatcggc acaatgccgc
    21181 ccatgatttt tgttccactt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat
    21241 ctcgatttta ttcactattt tgccgtcgcg aacgagggta atgcgatcga gtaatttacg
    21301 gcaatgcgcg caatgggtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg
    21361 tcttttcagg tgctgcacca tcattaagaa ctcctggtag tcaaaattaa agtgcggcat
    21421 ttactgacgt tataacttac ccagtttagt gcgtaaccgt ttaatggcct ggctgtgtaa
    21481 ctgactgacc cgcgattccc cgacctccag cactgcacca atctctttga gattcagctc
    21541 ttcctggtaa tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatagcttc
    21601 catcacccgc tggcgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg
    21661 atcatcagta accagttcaa tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa
    21721 gagctggctg ttattggtgt cgagcaacat ttggcgatag tcggcaatat cgatccctaa
    21781 acgttccgct acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat
    21841 tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg
    21901 aagttcatcc agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc
    21961 ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag
    22021 atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac
    22081 cagcgggaca taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag
    22141 tgaattcacg ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc
    22201 agggttttta atcggacgat tagtgggtga aatgaggggt tatttggggg ttaccggtaa
    22261 attgcgggca gaaaaaaccc cgccggtagc ggggtcaggc aggttaaggc cattagcctt
    22321 gtaacagaga cagaacttgc tgcggtacct ggttggcttt agacagcacg gagttgcccg
    22381 cctgctggat aatctgcgct ttcgacatgt tggacacttc ggtcgcatag tcggcgtcct
    22441 gaatacggga ctgcgcttca gacaggttgg tggtggtgtt gttcaggttg gtgaccgcag
    22501 aatccagacg gttttgaacg gcaccgaggg aggagcggaa tttgtcgact ttagcgattg
    22561 cggcatccag tgcttttaac ggatcggctg tagcggctct cgccacttcc gctgcaccaa
    22621 cagttaccgc cgccgccgta ccggtaatga ctacagtggc ttccatgtat ttaccggttg
    22681 cagaatcaac gaccgcatat ttagtactgt tagacgcggt gtcagtgtaa gacagtaaat
    22741 tgacttgttt acccccagca tccttgacat ccgcagcagc aattgctgta tcatcagcag
    22801 ccgtcagttt cgccgcgctg aaatccgcca tgccggacac ggtaaatcct ttcagattca
    22861 aagtggtact gtcgatttgt tgcaagccaa tgctgatcgt ctggttatca ttggcaccaa
    22921 tctgaatctt catcgaatcg tttttcgaca gcacgttcac accgttgaac tgggtctgac
    22981 cagatacacg gtcaatttca tcaagacggg atttgatttc gtcctgaata gaagacaggt
    23041 cggaatcaga gttcgtgccg gtagaggcct gaacggtcag ttcacgaata cgctgcaagt
    23101 tgttgttaat ctctgacagc gcgccttcag ccgtctgcgc cagagaaata ccgtcgttgg
    23161 cgttacgtgc ggcctgagtc agacctttga tgttagaagt aaagcggtta gcaatcgcct
    23221 ggcccgcggc gtcatcttta gcgctgttaa tacgcagacc agaagagagg cgctcgatag
    23281 aagtcgacag cgcagactgg tttttgttga tgttgttctg agtgatcagc gagaggctgt
    23341 tggtattaat gacttgtgcc ataattcatt ttcctgtttt caagtctacg gtttaagtat
    23401 tgggcttcca cccttcggct tcgtcgccgt caaccctgtt atcgtctggt cactcacaac
    23461 ctttaaaatt tttttctagc ggtcgaaata ctccccttcc tcgttgctgt tcgtgccatt
    23521 aaggcaaaaa ataaatcgta aactttaccc cgcttacgcc gataaccccg gtattcgttc
    23581 tatacacgtc gcatgattaa ggaaactata tggctagtat ttcaacgctt ggggttgggt
    23641 cggggctgga cttaagcagc atccttgaca gtctggaggc ggcagaaaaa tctaccctga
    23701 cgccgatatc aaaacagcaa agctcttaca ccgcgaaact gagcgcctac gggacgctga
    23761 aaagtgcact agagagtttc cagacagcca acacagcact gaataaagcc gatttattta
    23821 ccgccacctc aaccaccagc agttcatcgg ctttcagcgc caccaccacg ggcagcgcga
    23881 ttgctgggaa atacaccatt agcgttagcc aactggcgca ggcacagacg ctgacaacaa
    23941 aaaatagtca aaaagacagt aaagccgcca tcgccaccag cgatagcgta ctgaccatcc
    24001 aacaaggcgg cggtaaagat ccggtcacga ttgatatcag cgcgggtaac tcgtcacttt
    24061 ccggcattcg cgatgccatt aacaacgcca aagctggtgt cagcgccagt atcatcaatg
    24121 tgggaaacgg cgaatatcgt ctgtccatca ccgctaacga tacgggcagc aataacgcta
    24181 tgaaacttag cgtcagcggc gacagcgccc tggaaagttt tatgggttac aacggaacgc
    24241 cgggtgatag cagcaatggc atgattgaaa gcgtcaccgc gcaaaacgcc aaactgacag
    24301 tcaataacgt tgagattgaa aacagcagta acactatcag cgactcactg gaagatatca
    24361 ccctcaacct gaatgacgtc accaccggta atcagacact gaccatcagt aaagacacat
    24421 ccaaagctga aaatgcggtt aaagcgtggg tggatgccta taacacattg caggatacat
    24481 tcagcagctt aaccaaatac accgctgtcg atgccggagc tgaaagtcag gactccagta
    24541 atggcgcatt actcggtgac tccacactgc gtaccatcca gactcagctt aaaaccttgc
    24601 tcgccaatac tcacagcagt tcaaactata aaacgctggc acagattggc attaccagcg
    24661 atgccagtac cggcaagctg gaaatcgcga ccgacaaact gcaaacggcg ctaaaaaatg
    24721 atgccgctgg tatcggtgaa atgtttatag gtgacggcaa aagtactggg gtaaccaccg
    24781 gcattagtaa caacctgacc agttggctct cttccaccgg gataatccag gcagcaaaag
    24841 atggcgtcag caaaacgctg aataatttga ccgaccagta caacgcagcc agtgaacgta
    24901 tcgatacctt aatgacgcgc tataaagccc agttcaccca attagatgtg ttaatgaact
    24961 cgctgaactc cactagtagc tatctgacac agcagttcga cacctcaaat agcaactcga
    25021 aataatcttc aggagagact atgtacgcgg caaaaggcac ccaggcctat gcacaaattg
    25081 gcgtcgaaag cgccgtaatg agcgccagcc aacagcagct ggtcaccatg ctatttgatg
    25141 gagtgctgag cgcactggtt agagcgcgcc tgtttatgca ggacaacaat cagcaaggca
    25201 aaggcgtctc tttatcaaaa gcgatcaaca tcattgagaa cggactgcgg gtgagtcttg
    25261 atgaagagag caaagacgaa ttaacccaaa acttgattgc tctttatagc tatatggtca
    25321 ggcgcttgct gcaagccaat ttacgtaacg atgtctccgc agtcgaagaa gtggaagcat
    25381 taatgcgcaa tattgccgat gcctggaaag agtctttact ctccccttct ttgattcagg
    25441 acccagtctg atgaacaatg caccgcattt gtatttcgcc tggcaacaac tcgtcgaaaa
    25501 aagccagctc atgttacgcc tggcaacgga agaacaatgg gacgaactca tcgccagcga
    25561 aatggcgtat gtgaatgcgg tgcaggagat cgcacacctg actgaagagg ttgccccgtc
    25621 cactacgatg caggagcagc tccgcccgat gctgcgcctg attctcgaca acgaaagcaa
    25681 ggtaaaacag ttattacaga ttcggatgga tgaactggcg aaactggtcg gtcagtcatc
    25741 ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc ggctttgtgc tggctccgca
    25801 ggataacctc ttttgaatct taatgagccg atggctcgcg aataatccga ttacggctac
    25861 gcttctaata ttccccttga atggagtcga agaatgcgta atcccacgct gttacaatgt
    25921 tttcactggt attacccgga aggcggtaag ctctggcctg aactggccga gcgcgcagac
    25981 ggttttaatg atattggtat caatatggtc tggttgccgc ccgcctataa aggcgcatcg
    26041 ggcggctatt cggtcggcta cgactcctat gatttatttg atctaggtga gtttgatcag
    26101 aaaggcagca tccctactaa atatggtgat aaagcacaac tgctggccgc cattgacgcc
    26161 ctgaaacgta atgacattgc ggtgctgctg gacgtggtag tcaaccacaa aatgggcgcg
    26221 gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg atgaccgtac gcaaattgac
    26281 gaagaaatca ttgagtgtga aggctggacg cgttacacct tccccgctcg tgccgggcaa
    26341 tactcgcagt ttatctggga tttcaaatgt tttagcggta tcgaccatat cgaaaaccct
    26401 gatgaagatg gcatttttaa aattgttaac gactataccg gagaaggctg gaacgatcag
    26461 gttgatgatg aactcggtaa tttcgattat ctgatggggg agaatatcga ttttcgcaat
    26521 catgccgtga cggaagagat taaatactgg gcgcgctggg tgatggaaca aacgcaatgc
    26581 gacggctttc gtcttgatgc ggtcaaacat attccggcat ggttttataa agagtggatc
    26641 gaacacgtac aggaagttgc gccaaagccg ttgtttattg tggctgaata ctggtcgcat
    26701 gaagtcgata aactgcaaac gtatattgat caggtggaag gcaaaaccat gctgtttgat
    26761 gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg gtcgcgacta cgacatgacg
    26821 cagatcttca ctgggacgct ggtggaagcc gatccttttc acgccgtaac cctcgttgcc
    26881 aaccacgaca cccaaccgtt gcaagccctc gaagcccccg tcgaaccgtg gtttaaaccg
    26941 ctggcgtatg ccttaatttt gttgcgggaa aatggcgttc cttcggtgtt ctatccggac
    27001 ctctacggtg cacattacga agatgtcggt ggtgacgggc aaacctatcc gatagatatg
    27061 ccaataatcg aacagcttga tgagttaatt ctcgcccgtc agcgtttcgc ccacggtgta
    27121 cagacgttat ttttcgacca tccgaactgc attgccttta gccgcagtgg caccgacgaa
    27181 tatcccggct gcgtggtggt catgtcgaac ggagatgatg gcgaaaaaac cattcatctg
    27241 ggagagaatt acggcaataa aacctggcgt gattttctgg ggaaccggca agagagcgta
    27301 gtgacagacg aaaacggcga agcgaccttc ttttgcaacg gcggcagcgt cagcgtgtgg
    27361 gttatcgaag aggtgattta aatccatccc cggcggcaag ccggggagat ttcattacgg
    27421 cagtggcgtc ggtaacggcg ctttatccag cgcggcggca cactctgccg tcgggcgatc
    27481 cacacgctcc atcgtcatac catcgtactc aatggtgtta ccgtcacgct caacctcgta
    27541 cagctcacgt tttacggtaa cgttagtgag atcatcagac atcaatgtca acttacccgg
    27601 cacggcaatc acccgctgcc actgacggca atccagggta tcgccctctt tagtgacaat
    27661 gaggctgcct atcgcttccg gactgaccaa cgcacgttgc ggccctttgg tctgccagta
    27721 gcccgccagc cagtccggcg cgggggtttt cacgacattg ttatagtttt cgacttcggc
    27781 acagcccgcc agcaacatga gcgcacctgc aattgcgagt tttttcatca ttcgtcctgc
    27841 atgagaagaa aagaggattg tggcattaaa gccctgatgt cgccagcctt tccaggttca
    27901 gagagaaatt tgatctaacc attatttttg tgaattatta ccccgaaaat aaatttgcat
    27961 ggtgtatgat tcgcgactct tgtttcaaac cttctgagtt cagaggctac attcatgtca
    28021 tggcagcaat tcaaacacgc ctggttgatt aaattctggg cgcccatccc cgcggtcatc
    28081 gcggcgggta ttctctccac ttactatttt ggcatcactg gcaccttttg ggctgtcaca
    28141 ggtgaattta cccgttgggg cggtcagctc ctgcagttgt tcggcgtcca tgctgaagag
    28201 tggggttatt ttaaaattat ccatctggaa ggatcgccat taacccgcat cgacggaatg
    28261 atgatcctcg gtatgtttgg cggctgtttt gccgcagcgc tgtgggccaa caatgtcaaa
    28321 ctgcgcatgc cgcgcagccg tatccgcatt atgcaggcca tcattggcgg tattatcgcc
    28381 ggttttggcg cacgtctggc aatgggctgt aacctggcgg cgttctttac cggaattcca
    28441 cagttctcgc tgcacgcctg gttctttgcc atcgccactg ccattggttc atggtttggc
    28501 gcgcgcttta cccttctgcc catcttccgt attcccgtga aaatgcagaa agtttctgct
    28561 gcctcaccgt tgacgcaaaa accggatcag gcgcggcgtc gttttcgtct cgggatgctg
    28621 gtctttttcg gcctgctggg ctgggcgctg ctgacggcga tgaaccagcc caaactgggg
    28681 ctggcaatgc tgtttggtgt cggctttggt ttactgattg aacgtgcgca aatctgcttt
    28741 acttcggcgt tccgcgatat gtggatcacc ggacgtaccc atatggcgaa agcaatcatt
    28801 atcggtatgg cggtaagtgc catcgggatc ttcagttacg tacagttagg cgttgaaccc
    28861 aaaatcatgt gggcgggacc aaacgcggta attggtggtt tactgtttgg ttttggcatc
    28921 gtgctggcag gcggctgcga aaccggctgg atgtaccgcg cggtagaagg ccaggtgcac
    28981 tactggtggg tcggtctggg caacgtgatc ggctcaacga ttctggcgta ctactgggat
    29041 gatttcgctc cggcgctggc caccgactgg gacaaaatca acctgctgaa aacctttggc
    29101 ccgatgggcg gcctgctggt gacatatttg ctgttgtttg ctgctctaat gttgattatt
    29161 ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga ctgctaagga gatcgcatga
    29221 aaaatatcgt tcctgattac cgtcttgata tggtgggtga accctgccct tatccggcag
    29281 tcgcaaccct tgaggcgatg ccgcagttaa aaaaagggga gatcctggaa gtggtgagcg
    29341 actgtccgca gtcgatcaat aatattccac tggatgcgcg taatcacggc tatacggtgc
    29401 tggatattca gcaagacggg ccgaccattc gttatttaat tcagaagtaa tccctcattc
    29461 ccgctgtggc ccacagcggg aatatcgttg cctctctatc acatagattt aacccgcccc
    29521 ctctcctatt cttttcagtg tgacctcagg aggtttctat gtgtggacgc tttgcccaat
    29581 cccaaacgcg tgaagattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct
    29641 acgatcccga acctattggc agatacaacg tcgcgccggg aaccaaagtc cttctgctca
    29701 gtgaacgtga tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt
    29761 gggataaacc gccgctgatt aacgcccgcg tagaaactgc cgcaaccagt cacatgttta
    29821 aaccgctctg gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa
    29881 aagaaggcga caaaaagcag ccttatttta tctatcgtgc tgatggacaa gctgttttta
    29941 tagccgcgat aggtagcaca ccgtttgagc gtggtgacga agccggaaga agtttttgta
    30001 agcccgctca gggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca
    30061 ttgcgtttca gtggcatttt tccactcaac caaccaggaa gccattttcc cgtaagggat
    30121 gtatttatta ccggcctcat gattctcatt ctgaagcgcc agagcacgat tgaaaacgaa
    30181 acgacatgcg cctgcgaagc gcctcatttc acgctcctgt tgaccacctg gtcttaaccg
    30241 gaatttgaat gcctgtagtc gtttcatggg ccaaatcata ctttggccta tgaaaaaaga
    30301 aaccgatatc cgacgcggaa ggcattgtgt tttcctgaag catgttcacc tagtcttcgt
    30361 tactaaatac cgacgtcaga tttttgacca tgacgcgaca gaaaaactac gcacttactt
    30421 ttcaaatgta tgtgctgatt ttgaagctga acgggttgaa atggatggcg gaccagatca
    30481 cgtccatttg ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct
    30541 caaaggcgta tcgagtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta
    30601 cgacaaaggc gttttgtgga gtcctggcta ttttgtcagt agctgcatct taacgtatca
    30661 actagattta ataacaacga tatccgtacc taaccggata tctaatgtca taagcatccc
    30721 tttaattatc ccctcaacct cttgtaactt tcccactctt gagctccaca tagtccaaac
    30781 gccggatgca aaaaaatcac acgtgcattt ctgaaattgt gatggaggaa gatagtatca
    30841 attatatgtg cttagaaacg tccccgatta gcgcagaagt caccggattg ttcagactcc
    30901 aataggttga gtatgccgta tttattttcc aaaataaaaa tcgatgctgt tgctccttca
    30961 taaaatactt ttactctctg gacgcttttc ttagctctct tggtgttatc ttaaaacgat
    31021 tatggaacct ttcagtaaaa cgagaaggac acttgtaacc attttccctg gcaatctcgc
    31081 tgatgggttt tactgtcgtt tgtatagcag acaacgcatt atttaacctc acgtcgtcca
    31141 atatactttg aaaacttact ccctcgcttg ccagacggcg atgcaatgtg gaaacagaaa
    31201 tgtagagata tctcgctacc ttgtttgctg tccattttgt tccaggttcg gatagcatta
    31261 ggttataaca acgacttatt aatgattgtt tactatctga taaaagtaaa caattaacat
    31321 gatgtgctcc taatgaaagt agaacgccca ttgctaagtg ttcctgaatt tgcgttgaga
    31381 agcctcggga aatagatgtt tttagttgct cccaacaata tattaactcg gggttctgag
    31441 gtaagcagaa acctgtcgtg ttacgaattt gatcagttat cgcataaaat ttttgaaact
    31501 tttcaattag atcaatggga taataaagca tttctgcaag ataaagacct gcttctggat
    31561 aattcgcaat ataaaattca tatccacagg gaaataatat tatctgatta ttatcaacag
    31621 ttagactatg cgtctcccaa ttgataactt tttttccctg acggatacga cataaagctg
    31681 gcataagagg cttaaccctg tgaatctcat gatgtttatg catccgtatt tcttcaatct
    31741 ttaggttagt cttatctttt gccagcatac tcttacccta ctttatctca taaactggtg
    31801 ttatctcagc ggttgcaatt ttattggcat taagcatata accaactaac gctccgctgg
    31861 aattagaatc cacaggaatt ttatcagttt ttagagccca tactttaaac tggtaatgat
    31921 ggggtttatc tcctttggga ggacatgctc caccaaatcc tgcataacca aaatcatttc
    31981 ggccttgaac agcaccagtc ggaagttttg taccatcacg tcttcctgca tcagcgggca
    32041 aatatgttac agttgctgga atattagcaa cagtccagtg ccaccatcca ctgcctgtag
    32101 gagcatctgg atcatacaca gttacggcaa agcttttggt gccttcagga gcaccagacc
    32161 aggttaatga gggcgatgta ttaccacctt cacacccaaa tccagaaaat acatgagacg
    32221 ttgtaagttg ctctcctgtt tttatttcat tactagtgac ctgaaatgct gcggcttgcg
    32281 cagaaaatgt tatgaatgcc aatacacttg aaacgataag ttttttcata aaaacctcat
    32341 tgttgtgacc tatcgttatt ttatttgatg tgccttgatc tcattatgca taaaggcgca
    32401 atgatcgcgc aaatgcaatc ataattataa aggtgccccg aacattagcc ataatatgca
    32461 caaatcagat tcctgttatc tcaaggataa ttatttttta atgcggtgca cttcgtgaca
    32521 acaagttaat gactaacaca ccggcacaaa tcaacatcat gcctatgata gctggcaggt
    32581 ccagtcgttg accaaaaaat ccccaggaaa gcaaactaat cagaacgata ccgacgcctg
    32641 accagatagc ataagcaatc cctgtaggta tataagccag cgtctgagct aataaccaga
    32701 atgatgcaca atagcaaata attgtgccaa cagagggcca taaccgtgta aacccctctg
    32761 aaaacttcat taaggttgta ccaatgacct ctgcaagtat tgcgccacca agataaatat
    32821 aagggttcat agcatattct ttcctgttca tactggagag aattgtacta cagtttgaac
    32881 acaactcacc gttttcatcc tggtgtatgt gtatccaggg ggctctggcg tggcaagaga
    32941 taaaggcagg aacaataatg atcagaagga accccaggct aacgaatgag ttcactcttc
    33001 ctgggagcaa agcgaagaca aaacatgtta tcgcgtttac ggatcgtgga tggcagaaaa
    33061 ctttcaggat caggtatcca ttttaaacca gaaattgagt gagtttgccc catccatgcc
    33121 ccacgctgtg ggatcggata taataaacaa gcatagtcaa caggttacaa aacctacacc
    33181 tgcatggaca tcacttcctg atacgccgcc accagcttat tacgcacctg aatccccatt
    33241 tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat cgtttagcgc cacgccgggc
    33301 tcaccgaggg tgaatttttc tgcctgcgtg cgggcggctg tttgtgtatc gcttatgcga
    33361 tcgagcgcgg cgtgcagctg cccggcaaaa ctaattgtcg gttgcggcag tgattcctgc
    33421 gcacgcgcac tcatcgccgt ggtctgtaac tggctgataa ccccttcaat cccctgtatc
    33481 gctgacattt tcatctcccg gataatttct ggtagcaaag cctaccagta agtcaataag
    33541 acaaaggcgc taaatagcaa caaaaaaacg ggtttattgg cggatagaaa aaaacgaaag
    33601 cacaaataat gggagcgtca atttttcgag tttgctgacc cgggagtgag tcttgttcca
    33661 ctttgccaat aacgccgtcc ataatcagcc acgaggtgcg cgatgaatgc ggctgcagcc
    33721 cagacaaaat ctcttgagtg gcttaatcgc ctgcgtgcga atccgaaaat tccattgatt
    33781 gttaccggtt ccgcggcagt ggcggtcatg gtcgcactga tcctgtgggc gaaaaccccc
    33841 gactaccgta cattattcag caatctttcc gatcaggatg gtggcgcaat tgtcagccaa
    33901 ctgacgcaaa tgaatattcc ttaccgcttc agcgaagcca gcggcgctat tgaggttccg
    33961 gcagataaag ttcacgaact gcgcctggcg caacaaggtt tgcccaaagg cggcgcggtc
    34021 ggtttcgaac tgctcgatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat
    34081 tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatagg cccggtaaaa
    34141 ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc
    34201 ccttctgcat cggtgacggt aaatctgtta cccggccgcg cactcgatga agggcaaatt
    34261 agcgctattg tacatctggt ttccagcgcg gttgctggtc tgccgccagg aaacgtcacg
    34321 ctggtggatc agggcgggca tctgttaacc cagtccaata ccagcgggcg cgatcttaat
    34381 gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta ttcagcggcg tattgaagcg
    34441 atcctgtcgc ctattgttgg taacggtaat attcacgccc aggtcacggc gcaactggac
    34501 ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg
    34561 gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc
    34621 gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcaccaat cagcacgcct
    34681 ccggcaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag tgggccgcgt
    34741 agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa
    34801 atgaacgtgg gcgatgtgca acgtctgtca gtcgcggttg tggtgaatta caaaaccttg
    34861 ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga agctctgacc
    34921 cgcgaggcga tgggcttttc tgaaaaacgc ggtgattcgc tcaatgttgt taactcgccg
    34981 ttcaatagca gtgacgaaag cggcggagca ctgccgttct ggcaacagca agcgtttatc
    35041 gatcagttgc tggctgccgg tcgctggttg ctggtactgc tggtggcgtg gttgctgtgg
    35101 cggaaagcgg tacgcccgca gctaacacgt cgcgccgagg cggtgaagac tgtgcagcaa
    35161 caggcgctgg cccgcgagga agtggaagat gcggtggaag tccgtctgag caaagacgaa
    35221 caactccaac aacggcgcgc taaccaacga ctgggggcag aagtcatgag ccagcgtatc
    35281 cgtgaaatgt ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat
    35341 aacgatcatg agtaacctga caggcaccga taaaagcgtc atcctgctga tgaccattgg
    35401 cgaagaccgg gcggcagagg tgttcaagca cctctcccag cgcgaagtgc aaacccttag
    35461 cgctgcaatg gcgaacgtca cgcagatctc caacaagcag ctaaccgatg tgctggcgga
    35521 gtttgagcaa gaagctgaac agtttgccgc actgaatatc aacgccaacg attatctgcg
    35581 ttcggtattg gtcaaagctc tgggtgaaga acgtgccgcc agcctgctgg aagatattct
    35641 cgaaacccgc gataccgcca gcggtattga aacgctcaac tttatggagc cgcagagcgc
    35701 cgccgatctg attcgcgatg agcatccgca aattatcgcc accattctgg tgcatctgaa
    35761 gcgcgcccag gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat
    35821 gttgcgtatc gccacctttg gcggcgtgca gccagccgcg ctggcggaac tgaccgaagt
    35881 actgaatggc ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac
    35941 ggcagccgaa attatcaacc tgatgaaaac gcagcaggaa gaagccgtta ttaccgccgt
    36001 acgtgaattc gacggcgagc tggcgcagaa aatcatcgac gagatgttcc tattcgagaa
    36061 tctggtggat gtcgatgatc gcagcattca gcgtctgttg caggaagtgg attccgaatc
    36121 gctgctgatc gcgctgaaag gggccgagca gccactgcgc gagaaattcc tgcgcaatat
    36181 gtcgcagcgt gccgccgata ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct
    36241 gtcgcaggtc gaaaacgaac agaaagcgat tctgctgatt gtgcgccgtc ttgccgaaac
    36301 tggcgagatg gtgattggca gcggcgagga tacctatgtc tgataatctg ccgtggaaaa
    36361 cctggacgcc ggacgatctc gcgccaccac cggcagagtt tgtgccaatg gtcgagtcgg
    36421 aagaaaccat tattgaagag gccgaaccca gccttgagca gcaactggcg caactgcaaa
    36481 tgcaggccca cgagcagggt tatcaggcag ggattgccga aggtcgccag caaggtcatg
    36541 aacagggcta tcaggaagga ctggcccagg ggctggaaca aggtctggca gaggcgaagt
    36601 ctcaacaagc gccaattcat gcccggatgc agcaactggt cagcgaattt caaactaccc
    36661 ttgatgcact tgatagtgtg atagcgtcgc gcctgatgca gatggcgctg gaggcggcac
    36721 gtcaggtgat cggtcagacg ccaacagtgg ataactcggc actgatcaaa cagatccaac
    36781 agttgttgca gcaagaaccg ttattcagcg gtaaaccaca gctgcgcgta cacccggatg
    36841 atctgcaacg tgttgatgat atgctcggcg caactttaag tttgcatggc tggcgcttgc
    36901 ggggcgatcc caccctccat cctggcggtt gcaaagtctc cgccgatgaa ggcgatctcg
    36961 acgccagtgt cgccactcgc tggcaagaac tctgccgtct ggcagcacca ggagtggtgt
    37021 aatgaccacg cgcctgactc gctggctaac cacgctggat aactttgaag ccaaaatggc
    37081 gcagttgcct gcggtacgtc gctacgggcg attaacccgc gctaccgggc tggtgctgga
    37141 agccaccgga ttacaattgc cgctcggcgc aacctgtgtc attgagcgcc agaacggcag
    37201 cgaaacgcac gaagtagaaa gcgaagtcgt cggctttaac ggtcaacggc tgtttttaat
    37261 gccgctggag gaagtggaag gtgtcctgcc cggcgcgcgt gtatatgcca aaaacatttc
    37321 ggcagaaggg ctgcaaagcg gcaagcagtt gccgctcggt ccggcgttat taggtcgtgt
    37381 tctggacggc agcggtaaac cgctcgatgg cctcccctcc cccgatacga cggaaaccgg
    37441 tgcgctgatt accccgccat ttaacccatt gcaacgtaca ccgattgaac atgtgctgga
    37501 caccggcgtg cgcccaatca atgccctgct taccgtcggg cgcgggcagc gtatggggct
    37561 gtttgccggg tccggcgttg gtaaaagtgt gctgctgggg atgatggccc gttacacccg
    37621 cgccgatgtc attgtcgtgg gtttgattgg tgaacgaggg cgcgaagtaa aagattttat
    37681 tgagaatatt ctcggtgccg aaggacgtgc acgctccgtg gtgattgccg ctccggcgga
    37741 tgtttctccg ctcctgcgaa tgcagggtgc cgcctatgcc acgcgcattg ccgaagattt
    37801 tcgcgatcgt ggtcagcatg tgttgctgat tatggactcc ctcacccgct acgcgatggc
    37861 ccagcgtgaa attgcgctgg cgattggcga accacccgcc accaaaggtt atccaccgtc
    37921 ggtgtttgcc aaattaccgg cactggtcga gcgtgccgga aatggcatta gcggcggcgg
    37981 ctcgattacc gcgttttata ccgtgctcac cgagggcgat gaccagcagg accccattgc
    38041 cgactccgcg cgggccatcc tcgacggtca cattgtgctg tctcgccgac tggcggaagc
    38101 cgggcactat ccggctatcg atattgaagc gtcgatcagc cgcgcaataa cggcgttgat
    38161 cagcgagcaa cattatgcgc gagtgcgcac cttcaaacag ctgttgtcga gttttcagcg
    38221 taaccgcgat ctggttagcg tcggtgcgta tgccaaaggc agcgatccga tgctcgataa
    38281 agccatcgcc ctgtggccgc agctggaggg ctatttacaa caaggcattt ttgaacgcgc
    38341 ggactgggaa gcgtctctcc aggggctgga gcgtattttc ccgacagtgt cataacccag
    38401 gagataacgg cagatggcag aacatggtgc gctggcgacc ctgaaagatc tggcagaaaa
    38461 agaggtagag gatgccgcgc gcctgctggg tgaaatgcgt cgcggatgtc agcaggcgga
    38521 agaacagctc aaaatgctga tcgattatca gaatgaatat cgcaataacc tcaatagcga
    38581 tatgagtgcc gggatgacca gcaaccgctg gatcaactat cagcagttta tccagacgct
    38641 ggaaaaagcc attactcagc atcgccagca acttaatcag tggacgcaga aagttgacat
    38701 tgccctgaac agctggcgag aaaagaaaca acgtttgcag gcctggcaga cactgcagga
    38761 acggcaatcc acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa agatggatga
    38821 gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc gcatagcgcc cttgattacc
    38881 gctgacgttg ataacgccac attgcctggc ggcaaagcca gcgatgctgc acaagatttt
    38941 ctcgcgttgt tgagcgaagc attaacaggt gaggcaacaa ccgacaaagc gactccccag
    39001 ttgctggtgg caacagataa gcccacggca aaaggcgagc cgctggtcag cgatattctt
    39061 tcagacgcgc aacaagcaga tttactgatc cctgtggatg aaacaccgcc tgtcatcaac
    39121 gacgaacaat ccacattaac accgttaacc accgctcaga cgataacgtt ggctgcggcg
    39181 gctgacaaca atacggcaaa agacgaaaaa gcggatgatc tgaatgaaga cgtcaccgca
    39241 agcctgagtg ccctttttgc gatgttgccg ggttttgaca atacgcccaa agtgactgat
    39301 gcaccgtcaa ccgtgttacc gacagagaaa ccaacgcttt tcacaaaact gacttctgcg
    39361 caactcacta cagcacagcc tgatgacggc cccggcacgc cagctcagcc attaacagcg
    39421 ctggtagcag aagcccagag taaagcggaa gtcatcagca caccttcacc ggtgaccgct
    39481 gccgccagcc cgctaatcac tccacaccag acacagccac tgcccaccgt cgccgcgcct
    39541 gtcttgagtg caccgctggg ttctcacgaa tggcaacaat cattaagcca gcatatttcg
    39601 ctgttcaccc gccaggggca acaaagtgca gagttgcgtc tgcagccaca ggatttaggt
    39661 gaagtgcaaa tctccctcaa agtggatgat aaccaggcgc aaatccagat ggtttcgccg
    39721 catcagcacg tacgcgccgc cctggaagca gcgctgccgg tacttcgtac gcagctggcc
    39781 gaaagtggca ttcagttagg gcaaagcaac atcagtggcg aaagctttag tggccagcag
    39841 caggccgctt cccagcaaca gcaaagccaa cgcacagcaa accatgaacc tctggcgggg
    39901 gaagacgacg atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac aggcaacagc
    39961 ggcgttgata ttttcgccta acgtcagagg tagcaccgta atccgcgtct tttccccgct
    40021 ttgttgcgct caagacgcag gataattagc cgataagcag tagcgacaca ggaagaccgc
    40081 aacacatgac tgattacgcg ataagcaaga aaagcaagcg atcgctttgg atcccgattc
    40141 tggtattcat taccctcgcg gcctgtgcca gcgcaggtta cagctactgg cattcgcatc
    40201 aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc
    40261 tggatacctt tacggtcaat ctgggcgatg cggatcgcgt gctttatatc ggcataaccc
    40321 tgcgactgaa agatgaagca acccgctcgc ggctgagtga gtatttgccg gaagtccgta
    40381 gtcgcttgct gttactgttt tcgcgtcagg atgctgccgt actggcgaca gaagaaggca
    40441 agaaaaacct gattgccgag attaaaacca cactttccac cccgcttgtt gccgggcaac
    40501 cgaaacagga tgtcaccgac gtgctgtata ccgcttttat tctgcgataa cgacatgggc
    40561 gatagtattc tttctcaagc tgaaattgat gcgctgttga atggtgacag cgaagtcaaa
    40621 gacgaaccga cagccagtgt tagcggcgaa agtgacattc gtccgtacga tccgaatacc
    40681 caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga gcgctttgcc
    40741 cgccattttc gtatggggct gttcaacctg ctgcgccgta gcccggatat taccgtcggg
    40801 gccatccgca ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg
    40861 aaccttatcc atctgaaacc gctgcgcggc acagggctgg tggtgttctc accgagtctg
    40921 gtgtttatcg ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa
    40981 ggccgcgagt ttacccatac agaacagcgc gtcatcaatc gcatgttaaa gctggcgctt
    41041 gaaggctata gcgacgcctg gaaggcgatt aatccgctgg aagttgagta cgtgcgttcg
    41101 gaaatgcagg tgaaatttac caatatcacc acctcgccga acgacattgt ggttaacacg
    41161 ccgttccatg tggagattgg caacctgacc ggcgaattta atatctgcct gccattcagc
    41221 atgatcgagc cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa
    41281 gatcagaact ggcgcgataa cctggtgcgc caggtgcagc attcacagct cgagctggtc
    41341 gccaactttg ccgatatatc gctacgcctg tcgcagattt taaaactgaa acccggcgac
    41401 gtcctgccga tagaaaaacc cgaccgcatc atcgcccatg ttgacggcgt cccggtgctg
    41461 accagtcagt acggcacact caacggtcaa tatgcgttac ggatagaaca tttgattaac
    41521 ccgattttaa attctctgaa cgaggaacag cccaaatgag tgacatgaat aatccggccg
    41581 atgacaacaa cggcgcaatg gacgatctgt gggctgaagc gttgagcgaa caaaaatcaa
    41641 ccagtagcaa aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg
    41701 gaacgttgca ggatatcgac ctgattatgg atattccggt caagctgacc gtcgaactgg
    41761 gccgcacacg aatgaccatc aaagagttat tgcgtctgac gcaagggtcc gtcgtggcgc
    41821 tggacggtct ggcgggcgaa ccgctggata ttctgatcaa cggttattta attgctcagg
    41881 gcgaagttgt cgtagtcgct gataaatatg gcgtgcggat caccgatatc attactccgt
    41941 ctgagcggat gcgccgcctg agccgttagt gatgaataac cacgctactg tgcaatcttc
    42001 tgcgccggtt tctgctgcgc cactgctgca ggtgagcggc gcactcatcg ccattattgc
    42061 cctgatcctc gctgctgcct ggctggtaaa acggctggga tttgccccta aacgcactgg
    42121 cgttaacggt ctgaaaatta gcgccagtgc ttcactgggc gcgcgtgaac gggttgtggt
    42181 ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc gcagggcaaa tcaatctgct
    42241 gcataaactt cccccttcag caccaacgga agagataccg cagaccgatt ttcagtcggt
    42301 tatgaaaaac ttgcttaagc gtagcgggag atcctgatgc gtcgtttgtt gtctgtcgca
    42361 cctgtccttc tctggctggt tacgcccctc gccttcgcgc aactgccggg tatcaccagc
    42421 cagccgctgc ctggcggtgg acaaagctgg tcgctcccgg tgcaaacgct ggtgttcatc
    42481 acctcgttga cgtttattcc ggcaatttta ctgatgatga ccagtttcac ccgcatcatc
    42541 attgtttttg gtttattgcg taacgcgttg ggaacaccct ccgcgccacc taaccaggta
    42601 ttgctgggac tggcgctgtt tttgaccttt tttattatgt cgccggtgat cgataaaatt
    42661 tatgtcgatg cgtaccagcc attcagcgaa gagaaaatct caatgcagga ggcgctggaa
    42721 aaaggggcgc agccgctgcg tgagtttatg ctgcgtcaga cccgcgaggc ggatttaggg
    42781 ttgtttgcca gactggcgaa tactggcccg ttgcagggac ctgaagctgt gccgatgcgc
    42841 attttgctcc cggcctacgt gaccagcgag ttgaaaaccg catttcagat aggcttcacg
    42901 attttcattc cttttttgat tatcgacctg gtgattgcca gcgtgttgat ggcattgggg
    42961 atgatgatgg ttcccccagc cacaattgct ctgcccttta aactgatgct gtttgtactg
    43021 gtggatggct ggcaattgct ggtcggttcg ctggcgcaga gcttttacag ctagagaggc
    43081 aaaatgacac ctgaatcggt catgatgatg gggactgaag cgatgaaagt cgcgctggca
    43141 ctggctgccc cactgttgct ggtggcgctg gtcacgggtc ttatcatcag tattttgcag
    43201 gccgccaccc agattaacga aatgacgctg tcgtttattc cgaaaatcat cgccgtattt
    43261 atcgccatta ttattgccgg accgtggatg ctcaatctgt tgctggatta cgtccgcacc
    43321 ttgttcacta acctgccgta tatcatcggg taggccgtac aatgatgcag gtaacaagcg
    43381 atcaatggct ttcctggtta agcctgtact tctggccgtt actgcgcgtg ctggcgctca
    43441 tttctaccgc accaattctg agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg
    43501 caatgatgat cacgttcgct attgccccat cattacctgc caacgatgtt cctgtttttt
    43561 cgttctttgc tctgtggctg gccgtgcagc agatcctgat cggcattgcg cttggtttta
    43621 ccatgcaatt tgcctttgcc gctgtgcgaa ccgctggtga aattatcggt ctgcaaatgg
    43681 ggctgtcatt tgcgacgttt gtcgatccgg ccagccatct caatatgccc gttttagcgc
    43741 gtatcatgga tatgctggcg ttactgctgt tcctgacatt taacggtcat ttatggttga
    43801 tttcactgct ggccgatacc tttcacaccc tgccaattgg tggcgaaccg ttgaacagca
    43861 atgcgtttct ggcactcacc aaagcaggga gtttgatttt ccttaacggg ctgatgctgg
    43921 cattaccgct cattactctg ctgctgaccc tgaatctggc attaggctta cttaatcgta
    43981 tggccccgca attatccatt tttgttattg gatttccatt aactctgact gtcggtatct
    44041 ctttaatggc ggcattaatg ccgttaattg cgcctttttg cgaacattta ttcagtgaga
    44101 tgtttaattt gctggctgat attattagtg aattgccatt aatataattc cgtaacgttt
    44161 atcatgttat cctaaggatt atccgaaaaa taatacctac gaacatcttc caggatactc
    44221 ctgcggcgaa atatttgttt taagctcact cacatatcgc aacatttact ttactttaag
    44281 acaattccag gcaaattata caacacttta cgggatagta agtccgcctg aaaaatcgcg
    44341 agagtggcgc attaggtgac ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa
    44401 ggggaattat cgttacgcat tgagtgaggg tatgccatgt caacgattat tatggattta
    44461 tgtagttaca cccgactagg tttaaccggg tatctgttga gtagaggggt taaaaaaaga
    44521 gaaatcaacg acattgaaac cgttgatgac cttgccatag cttgtgattc acagcgccct
    44581 tcagtggtgt ttattaatga ggactgtttc atccacgatg cttctaacag tcagcgtatc
    44641 aagctcatca ttaatcaaca tcccaatacg ttatttatcg tttttatggc aattgccaat
    44701 gttcattttg atgaatatct attggtcaga aaaaatttat tgatcagttc taaatcgatt
    44761 aaaccggaat ctctcgacga tatccttggc gatattctga aaaaagagac aacgataacc
    44821 tcgtttttaa atatgccgac gttatcattg agccgaaccg aatcgagtat gttgcgaatg
    44881 tggatggcag gtcagggaac cattcaaatc tctgaccaaa tgaatatcaa agccaagacc
    44941 gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa cgcataataa acaggttatc
    45001 taccatgtcg tccgactgac ggataatgtg actaatggta tttttgtcaa catgcgctaa
    45061 cacattctga ctggtggttt cccaccagtc aggccgaatt agattactct gctttctcca
    45121 caaagatacc gtcctgatgc cctgcttcat taaagaacca gatgccgagc gggtagtctt
    45181 ccagcgaaac caggtacatt gtgccttcac taaactcctc aactgccagt accacgccag
    45241 gacgacgcgg accgccatcc gttttgactg ttacccgatc attcaccttc atcgttttcc
    45301 tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc aggcgcgcgc
    45361 gagtgctgat tttctcgacg gtctatactt aagagatgcc aggcggactt aacgactggc
    45421 cgcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag agtacagcga
    45481 taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga tcaatgaaat
    45541 tagcgaaagc gaagttcatc gcagccagaa cgattctgaa cacgttagcg tcgatggacg
    45601 tgaatatcat acatggcgtg aattggcgga tgccttcgaa ctggatattc atgacttcag
    45661 cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga gggggtcggg
    45721 atgaaacttg cttaagcaag aagcacttaa aaaattcgtt acaccaggaa atctgatgtg
    45781 ttcatcacct tatccgcaat ttttttcgct gacaagaaaa tattcgtgat atgaatgatt
    45841 aacgctactt atgaatagaa atgtgacgcg cgtcacccct tggtggctct ggcgtcaacg
    45901 ggatcgctcc cgaaaaaatc atctttgata atttgtgctg ttttgagaat catcctggta
    45961 aggggagtac tgcgcaacca tgctttcaat tcaacaacca ctactggttt ttagcgatct
    46021 tgatggcacc ctgctggaca gtcatagtta tgactggcaa ccagctgccc cctggctcag
    46081 ccgtttacgc gaagcgaatg ttcccgtcat tctctgtagc agtaagacat cagccgaaat
    46141 gctgtacttg caaaaaacgt tggggctaca aggtttaccg ctgattgccg aaaatggcgc
    46201 agtgatccag cttgctgagc aatggcagga tatagacggt tttccacgca tcatctcagg
    46261 tattagccat ggcgaaatca gccaggtttt aaatacgcta cgcgagaaag aacattttaa
    46321 attcacaact tttgatgatg tcgacgatgc aaccatcgcc gaatggacgg gattaagccg
    46381 tagccaggcg gcgctgacgc agctacatga ggcgtcggta acgctaatct ggcgcgacag
    46441 tgacgagcgt atggcacaat ttaccgctcg tctgaacgaa ctgggcttgc agtttatgca
    46501 gggtgcgcgc ttctggcacg tcctggatgc ctctgccgga aaagatcagg ctgccaactg
    46561 gattatcgcg acctatcaac aatcgtcagg caaacgccca accacacttg gcctgggcga
    46621 tgggccaaac gatgcgccct tactggaggt aatggattac gcggtgattg tgaaagggtt
    46681 aaaccgtgaa ggggtgcatc tgcatgatga ggatccggcc cgcgtctggc gaacgcagcg
    46741 tgaaggaccg gaaggctggc gtgaagggct ggaccatttt ttctccgccc attaagcgtt
    46801 atcgctcgcg aatacccgat tgcgcccggc ctgtttagcg agataaagtc gacggtcggc
    46861 cagtgactgg agttgttcaa aatcataatc accggtttcc tcgctgctgc ttacccccag
    46921 cgaggcactg atgcgtatcg tcgtactctt ggcgatcaac atctcttttt catttagctt
    46981 caggcgaata cgttctgcaa cctccgcagc ctgcgtcagg ctcgcgcctg gcagaatcac
    47041 acaaaactcc tcaccaccga cccgcccggc aacatcctgc gctcgcaagg aactgctaat
    47101 taatccggca gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc ggtcattaat
    47161 cgctttaaaa tggtcaaggt cgacctgaat gacagaaaaa ggatgttggt gcgtctgaca
    47221 caatttagcg agcggacggg ctttttcgaa cagtgcgcca cggttatata aacgcgttaa
    47281 ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaaa acatacatgt tgctgaccat
    47341 ccggcgaatc acataccagg agatgagtaa catggtggta aagagcgccc acagcagggt
    47401 taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg tatggacgcg
    47461 caccagcaca ccgtcgaaat ggtccagacg ttcccagcta acatagcgac tgttcatacg
    47521 aatgcagccc cgcgtgtcat gttccattgc ctgcgccagc aaggccagtt cacgaggatc
    47581 aaaaatattc cctgttggat ggtcaggatt ggaagaggtc aataatctca gcttactgtc
    47641 atagagctga tactcaccat cgaggttttt atcgatggcg tttcttaaaa attgctgcat
    47701 ggtacgcacg ggaatactca tccccagcac gccataccag tagttttcac tgtctaccgg
    47761 aacactgacg gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg aagtgaacca
    47821 gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg acaaaccagg gttgggtgac
    47881 atagccgtaa taacgcgttg gtacattgcg cgtaaacaag gtcggctgcg tcgaaacgta
    47941 aaatccggca cgcgagacat acatcgcctg ttcaaccatc gacgaggagt tgtgtgccag
    48001 tcgcagcaag taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc tttcattttc
    48061 gcgagacagg agattcccct cgctgactaa tgcatccgac acaccattga ctggcagggt
    48121 gcgtcgtcgg ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt cgaactcggt
    48181 tacggcatta cgcagagagg tgaaatccag tggcgctacg agagcttcgc gcatgccatt
    48241 acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg cgttggctac
    48301 gttttccaga tgattacgct ggctggagat ataggcatct tccagcacca ccacttcacg
    48361 ccaggtgagc aaggtggaaa aaagcaatac cacgataaag cagagattaa cgacatgacc
    48421 aggccccagg cggcgtgcga gttttttcaa ccagctctgg ttttccattt ttgtctcgtg
    48481 ctgcaccctg acgactccca acctgctttt ttatgattct ggcataacct ggcgatagcg
    48541 taccgcaaaa cagcgataat tttataatgg tggcatgata agacgcgata gcgtcgcatc
    48601 aggcaattca gtgcagacac tggcacaccc gaccggatgc gacgtgagcg ccacatccgg
    48661 tcgggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct cccccggaac
    48721 aagctcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc aacgacactc
    48781 aaccataccg ctggagtatc cggtgacaat catccgcggg ccgccctctt taaccgtaac
    48841 ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac ttgttcacct
    48901 tagttgaaga tggcgaattt tgcataatag cctgtgcgag tattaatgtg cctgaccgcg
    48961 tattttcgct acggctttca ctcccagcac cacgatgccg ccgatgataa atccaagaat
    49021 cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc cggcgaaatg
    49081 ttcaatggcg tgatgcagcg gcgcaattcc atgtaccaca atcccaccgc cgacgaggaa
    49141 catcgccagc gtgccgacaa tcgataacgc cttcatcaac cagggcgcaa tgatcaataa
    49201 ccctttacct aatgcctgca tcagcgcgct ggatttttcc gccagccaat accccaggtc
    49261 atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta ccagcgcgat
    49321 gcctgaaagc accagcacct gattaagcaa cggcgcttcg gccacaatcc ccagcgtgat
    49381 ggcgacgatt tccgcagaca atataaaatc ggtacgaatc gcccctttta ttttgtcttt
    49441 ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctgac tctgcgccgg
    49501 atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc cttcaaagca
    49561 gagaaacgcg ccgccaatca tcaacagtgg cgtaatcgcc cacgggataa acgcactgat
    49621 gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg ccacgcccca
    49681 gaccacggga agttcccggt tggcgcgcac gcctgaaact tgttgcgcat tgagcgataa
    49741 gtcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca cggagatatc
    49801 gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg ccaaaagcat
    49861 tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca gctctgatac
    49921 ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct cgccagcaga
    49981 atgattttca cgtttactat gaaccacctt tttatttcag cagtgaggga ttatgcgttt
    50041 ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt ggggctcaac
    50101 ctattttgtc attcggattg gcgtggaaag ctggcctccg ttaatgatgg cgggcgttcg
    50161 attcctggca gccggtattt tattgctggc atttttgcta ctgcgcggac acaaacttcc
    50221 cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg ctgtcggtaa
    50281 tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg ccgttgtggt
    50341 tgcaaccgtg cccctcttta ccctgtgctt cagccgcctg tttggcatta aaacgcgcaa
    50401 actggaatgg gtgggtatcg ccattgggct taccggaatc atcatgctca atagcggtgg
    50461 aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga ttagctgggc
    50521 gtttggctcg gtttatggct cgcgcattac cttacctgta gggatgatgg cgggtgcgat
    50581 tgagatgctg gcggctggcg tggtgttaat gattgcgtcg atgattgcgg gtgaaaaact
    50641 gacggcgctc ccttcccttt caggcttcct tgcggtcggc tatctggcgc tgtttggttc
    50701 gattatcgcc attaacgctt atatgtattt aatccgcaac gtcagtccgg ctctcgccac
    50761 cagctacgcc tacgttaacc cagtggtcgc ggtcttgctg ggtacggggc tgggtggcga
    50821 aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gttttcgcgg tggtactggt
    50881 cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta ttccggatgc
    50941 atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct tcgccgcaga
    51001 tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc ccgcgtaacg
    51061 cacattccca gacaatcaat acgcgccaac cgagttcctg caaatggctg atatcacgac
    51121 gatcgcgctc gacattttta cctatcttct ccagccaaaa atctgttcgc gtggcgggga
    51181 ctttaaacag gtagcaatgg tgatggtgcc agaagcaacc gtgggcaaat atcacacagc
    51241 gaaattcatc aaggacaaaa tccgggcgtc cgggcagact ggcgtcctga acgcgaaacg
    51301 ccaggccctg cccagttaac aaactggcga ggcgcttctc aatcgccgta tcacgcgtgg
    51361 caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc atgttgtgcc
    51421 tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt tgccaccgcg
    51481 gcaaagaccg gcacgaccac cgagttaccg aactggcgat aagcctgagt gtccgaaacc
    51541 ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc acattcccga
    51601 ggcgttaacc gacgtgggcg atgttgctga ttcagcggat cgtcaaagtc tttctcaccc
    51661 gttgccatat cccagccgcg atcgattaaa atttccgcgc catctttgta ataacgcgca
    51721 gacagcgtac gcgtgacgct ttgcggattg ttcggataaa ccattccata accgaagccg
    51781 ttaccgcgca cctgatgttt tttcgcatat cgatagaggt acttccacag taccggcgtc
    51841 aggatatatt tcgcctcgac catcgggtcc agcagctgcg ccagcgtcac tcgctgtgca
    51901 gggaaacatt tgctgatatc acgcagagta aaatcggctt tcagattcag atcgcgacga
    51961 aaacccacca gcacgatgcg ttcacggtgc tgcggcagaa aatgtttgcc gtcgatgatt
    52021 ttcggatcgt ccggcccgtt atcttctgca tcagccacgt catagcccag ttcgtccagc
    52081 gtctgcatga tgatgcggaa cgttttaccc tggtcgtgac ttttcaggtt tttgacgttt
    52141 tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc aaagaacagc
    52201 gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt tttcgatacg
    52261 ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg ttcaggaatg
    52321 tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg gctgagggtg
    52381 atgtcgcgga tatcctcatt aaaatgatgc gtcgccgggt cgcaataatg gttggcttta
    52441 taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc accaatcgat
    52501 tcaaaaccgc gacggatgcc gccaattccg gcaaatagat cgataaagcg aaacgcataa
    52561 tgcggatgat gtgccggtgg tttgggtaac aacgtttgca gatgggcgaa ctctttctca
    52621 cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc cgcgctccag
    52681 tgattctcac ccacaccatt aagctgcgcc accagcgttt taacatcata aatttgcagc
    52741 agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt tactgatata
    52801 ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca taattactgc
    52861 cgtttagggc cacgcgctca gccagcaggt gaaaacgcat ctatcacctt gtgatcgata
    52921 ccttcattct cccccgccag ttcggcaccg agcttcgcca taaactcgac taaaaattgc
    52981 gcgttgtgct gtgccagctg cttgcctctt gcggtttgca tggtttgcgg taatttcagt
    53041 aactttgtct ggaaatgatc cagcgcgtag cgtttatcat caagcgggcg atgctgtgcg
    53101 aacggatctt cgccatcaaa cagcgccaag cccaatgccc ctgaaacggc aaatacgcgc
    53161 gccaggccaa tcgcccccag ggcttccagc cgatccgcat cctggacgat tttagcttcc
    53221 gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg caatggcatg acaaacggcc
    53281 tcaattttct ccgccggaaa ttgcacgaac tcttcggtaa tgctgccaac ttactgattt
    53341 agtgtatgat ggtgatttta aggtgcttgc gtggcttcca tttccatcag atgtccttcc
    53401 tgctccgc
//
LOCUS       DBBGLQ010000031        49686 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.031, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000031 DBBGLQ010000000
VERSION     DBBGLQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.031"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(98..211)
                     /locus_tag="ACIYSB_002311"
     CDS             complement(98..211)
                     /locus_tag="ACIYSB_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGC5429354.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            complement(259..1377)
                     /gene="solA"
                     /locus_tag="ACIYSB_002312"
     CDS             complement(259..1377)
                     /gene="solA"
                     /locus_tag="ACIYSB_002312"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGC5429355.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            complement(1492..1746)
                     /gene="bssS"
                     /locus_tag="ACIYSB_002313"
     CDS             complement(1492..1746)
                     /gene="bssS"
                     /locus_tag="ACIYSB_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGC5429356.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            complement(2034..2279)
                     /gene="dinI"
                     /locus_tag="ACIYSB_002314"
     CDS             complement(2034..2279)
                     /gene="dinI"
                     /locus_tag="ACIYSB_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGC5429357.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            complement(2353..3399)
                     /gene="pyrC"
                     /locus_tag="ACIYSB_002315"
     CDS             complement(2353..3399)
                     /gene="pyrC"
                     /locus_tag="ACIYSB_002315"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGC5429358.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            complement(3505..4065)
                     /gene="yceB"
                     /locus_tag="ACIYSB_002316"
     CDS             complement(3505..4065)
                     /gene="yceB"
                     /locus_tag="ACIYSB_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGC5429359.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQVLRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            complement(4199..4846)
                     /gene="grxB"
                     /locus_tag="ACIYSB_002317"
     CDS             complement(4199..4846)
                     /gene="grxB"
                     /locus_tag="ACIYSB_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGC5429360.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            complement(4910..6118)
                     /gene="mdtH"
                     /locus_tag="ACIYSB_002318"
     CDS             complement(4910..6118)
                     /gene="mdtH"
                     /locus_tag="ACIYSB_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGC5429361.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            6354..6938
                     /gene="rimJ"
                     /locus_tag="ACIYSB_002319"
     CDS             6354..6938
                     /gene="rimJ"
                     /locus_tag="ACIYSB_002319"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGC5429362.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            6949..7596
                     /gene="yceH"
                     /locus_tag="ACIYSB_002320"
     CDS             6949..7596
                     /gene="yceH"
                     /locus_tag="ACIYSB_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGC5429363.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     NRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            7598..8521
                     /gene="yceM"
                     /locus_tag="ACIYSB_002321"
     CDS             7598..8521
                     /gene="yceM"
                     /locus_tag="ACIYSB_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGC5429364.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            8631..10166
                     /gene="murJ"
                     /locus_tag="ACIYSB_002322"
     CDS             8631..10166
                     /gene="murJ"
                     /locus_tag="ACIYSB_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGC5429365.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(10206..10622)
                     /gene="flgN"
                     /locus_tag="ACIYSB_002323"
     CDS             complement(10206..10622)
                     /gene="flgN"
                     /locus_tag="ACIYSB_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGC5429366.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(10627..10920)
                     /gene="flgM"
                     /locus_tag="ACIYSB_002324"
     CDS             complement(10627..10920)
                     /gene="flgM"
                     /locus_tag="ACIYSB_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGC5429367.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            complement(10996..11655)
                     /gene="flgA"
                     /locus_tag="ACIYSB_002325"
     CDS             complement(10996..11655)
                     /gene="flgA"
                     /locus_tag="ACIYSB_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGC5429368.1"
                     /translation="MLIIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            11810..12226
                     /gene="flgB"
                     /locus_tag="ACIYSB_002326"
     CDS             11810..12226
                     /gene="flgB"
                     /locus_tag="ACIYSB_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGC5429369.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            12230..12634
                     /gene="flgC"
                     /locus_tag="ACIYSB_002327"
     CDS             12230..12634
                     /gene="flgC"
                     /locus_tag="ACIYSB_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGC5429370.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            12646..13341
                     /gene="flgD"
                     /locus_tag="ACIYSB_002328"
     CDS             12646..13341
                     /gene="flgD"
                     /locus_tag="ACIYSB_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGC5429371.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            13366..14574
                     /gene="flgE"
                     /locus_tag="ACIYSB_002329"
     CDS             13366..14574
                     /gene="flgE"
                     /locus_tag="ACIYSB_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGC5429372.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            14755..15345
                     /gene="flgF"
                     /locus_tag="ACIYSB_002330"
                     /pseudo
     CDS             14755..15345
                     /gene="flgF"
                     /locus_tag="ACIYSB_002330"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            15517..16299
                     /gene="flgG"
                     /locus_tag="ACIYSB_002331"
     CDS             15517..16299
                     /gene="flgG"
                     /locus_tag="ACIYSB_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGC5429373.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            16352..17050
                     /gene="flgH"
                     /locus_tag="ACIYSB_002332"
     CDS             16352..17050
                     /gene="flgH"
                     /locus_tag="ACIYSB_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGC5429374.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            17062..18159
                     /gene="flgI"
                     /locus_tag="ACIYSB_002333"
     CDS             17062..18159
                     /gene="flgI"
                     /locus_tag="ACIYSB_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGC5429375.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            18159..19100
                     /gene="flgJ"
                     /locus_tag="ACIYSB_002334"
     CDS             18159..19100
                     /gene="flgJ"
                     /locus_tag="ACIYSB_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGC5429376.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            19166..20809
                     /gene="flgK"
                     /locus_tag="ACIYSB_002335"
     CDS             19166..20809
                     /gene="flgK"
                     /locus_tag="ACIYSB_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGC5429377.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            20821..21774
                     /gene="flgL"
                     /locus_tag="ACIYSB_002336"
     CDS             20821..21774
                     /gene="flgL"
                     /locus_tag="ACIYSB_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGC5429378.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLSK"
     gene            complement(21970..25155)
                     /gene="rne"
                     /locus_tag="ACIYSB_002337"
     CDS             complement(21970..25155)
                     /gene="rne"
                     /locus_tag="ACIYSB_002337"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGC5429379.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            25728..26687
                     /gene="rluC"
                     /locus_tag="ACIYSB_002338"
     CDS             25728..26687
                     /gene="rluC"
                     /locus_tag="ACIYSB_002338"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGC5429380.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            complement(26799..27383)
                     /gene="yceF"
                     /locus_tag="ACIYSB_002339"
     CDS             complement(26799..27383)
                     /gene="yceF"
                     /locus_tag="ACIYSB_002339"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGC5429381.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            27582..28103
                     /gene="yceD"
                     /locus_tag="ACIYSB_002340"
     CDS             27582..28103
                     /gene="yceD"
                     /locus_tag="ACIYSB_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGC5429382.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            28155..28328
                     /gene="rpmF"
                     /locus_tag="ACIYSB_002341"
     CDS             28155..28328
                     /gene="rpmF"
                     /locus_tag="ACIYSB_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGC5429383.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            28409..29479
                     /gene="plsX"
                     /locus_tag="ACIYSB_002342"
     CDS             28409..29479
                     /gene="plsX"
                     /locus_tag="ACIYSB_002342"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGC5429384.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            29547..30500
                     /gene="fabH"
                     /locus_tag="ACIYSB_002343"
     CDS             29547..30500
                     /gene="fabH"
                     /locus_tag="ACIYSB_002343"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGC5429385.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            30516..31445
                     /gene="fabD"
                     /locus_tag="ACIYSB_002344"
     CDS             30516..31445
                     /gene="fabD"
                     /locus_tag="ACIYSB_002344"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGC5429386.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            31458..32192
                     /gene="fabG"
                     /locus_tag="ACIYSB_002345"
     CDS             31458..32192
                     /gene="fabG"
                     /locus_tag="ACIYSB_002345"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGC5429387.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            32403..32639
                     /gene="acpP"
                     /locus_tag="ACIYSB_002346"
     CDS             32403..32639
                     /gene="acpP"
                     /locus_tag="ACIYSB_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGC5429388.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            32727..33968
                     /gene="fabF"
                     /locus_tag="ACIYSB_002347"
     CDS             32727..33968
                     /gene="fabF"
                     /locus_tag="ACIYSB_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGC5429389.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            34088..34897
                     /gene="pabC"
                     /locus_tag="ACIYSB_002348"
     CDS             34088..34897
                     /gene="pabC"
                     /locus_tag="ACIYSB_002348"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGC5429390.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEESLQADEMVICNALMPVMPVCACGDVSFSSATLYEYLAPLCERPN"
     gene            34900..35922
                     /gene="yceG"
                     /locus_tag="ACIYSB_002349"
     CDS             34900..35922
                     /gene="yceG"
                     /locus_tag="ACIYSB_002349"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGC5429391.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDKVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            35912..36553
                     /gene="tmk"
                     /locus_tag="ACIYSB_002350"
     CDS             35912..36553
                     /gene="tmk"
                     /locus_tag="ACIYSB_002350"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGC5429392.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            36550..37554
                     /gene="holB"
                     /locus_tag="ACIYSB_002351"
     CDS             36550..37554
                     /gene="holB"
                     /locus_tag="ACIYSB_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGC5429393.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            37565..38362
                     /gene="ycfH"
                     /locus_tag="ACIYSB_002352"
     CDS             37565..38362
                     /gene="ycfH"
                     /locus_tag="ACIYSB_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGC5429394.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRCLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            38657..40090
                     /gene="ptsG"
                     /locus_tag="ACIYSB_002353"
     CDS             38657..40090
                     /gene="ptsG"
                     /locus_tag="ACIYSB_002353"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGC5429395.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(40150..42338)
                     /gene="fhuE"
                     /locus_tag="ACIYSB_002354"
                     /pseudo
     CDS             complement(40150..42338)
                     /gene="fhuE"
                     /locus_tag="ACIYSB_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            42672..43031
                     /gene="hinT"
                     /locus_tag="ACIYSB_002355"
     CDS             42672..43031
                     /gene="hinT"
                     /locus_tag="ACIYSB_002355"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGC5429396.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            43034..43411
                     /gene="ycfL"
                     /locus_tag="ACIYSB_002356"
     CDS             43034..43411
                     /gene="ycfL"
                     /locus_tag="ACIYSB_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGC5429397.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPFLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            43425..44066
                     /gene="lpoB"
                     /locus_tag="ACIYSB_002357"
     CDS             43425..44066
                     /gene="lpoB"
                     /locus_tag="ACIYSB_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGC5429398.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            44047..44871
                     /gene="thiK"
                     /locus_tag="ACIYSB_002358"
     CDS             44047..44871
                     /gene="thiK"
                     /locus_tag="ACIYSB_002358"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGC5429399.1"
                     /translation="MPFRSNNPITRDELLSRFFPQYHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            44882..45907
                     /gene="nagZ"
                     /locus_tag="ACIYSB_002359"
     CDS             44882..45907
                     /gene="nagZ"
                     /locus_tag="ACIYSB_002359"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGC5429400.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNRLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            45930..46472
                     /gene="ycfP"
                     /locus_tag="ACIYSB_002360"
     CDS             45930..46472
                     /gene="ycfP"
                     /locus_tag="ACIYSB_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGC5429401.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            46872..48176
                     /gene="ndh"
                     /locus_tag="ACIYSB_002361"
     CDS             46872..48176
                     /gene="ndh"
                     /locus_tag="ACIYSB_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGC5429402.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            48386..48925
                     /gene="ycfJ"
                     /locus_tag="ACIYSB_002362"
     CDS             48386..48925
                     /gene="ycfJ"
                     /locus_tag="ACIYSB_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGC5429403.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(48987..49619)
                     /gene="comR"
                     /locus_tag="ACIYSB_002363"
     CDS             complement(48987..49619)
                     /gene="comR"
                     /locus_tag="ACIYSB_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGC5429404.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPPHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
BASE COUNT        11936 a        12335 c        13374 g        12041 t
ORIGIN      
        1 ctggtgagtg ctgtagaagc aggaataatc tcattcaatt tttcacgcta aatgaaaggg
       61 agattaaata tttgtcagtt tatgtcagtg cggaaaacta aaaataaaac acatagatca
      121 gatccataat tgccaacaat ccccagagaa aaagataaag catcagatgc tcgcgaatat
      181 tattgatgag atagtgcaac agccgacgca ttaaatactc ctgtaaaaac ggcctcattc
      241 tggaggccgt aatgatgatt attggaagcg ggaaagcctg aatggcgtca aatcaaaatc
      301 gctttttttg tcttgcgcaa aatcggcagc tatttcccct aaaactgacg caaatttaaa
      361 accgtgccca ctcaggccgg taatgagcag tgtattatcg tggccgggta gggtatcgat
      421 aataaaatct tcgtcaggcg aattatcata ggtgcaggca gcgccgtaca ggcagcaacc
      481 gatacccggc aatacattgc gcaagaacgg gaaggcttct gacccatcac tggccacttc
      541 cgcaaacgga acacgttcat ccgctgaatg gataacctgg cctccgttat gtttgccaat
      601 cttcaacgcg tcgttttctg ccggaaaacc ataatattga tcgccattgg gcagttcacc
      661 ggtaaacgcc gggaatttat tcttcacgct atagcggcca tcggcctgat accaggcaaa
      721 tactttgcgt acgggctgga caggcagctc cgggagcagg tcttttaccc atgttcccgc
      781 gcagacaatc gcttttttcg cctgatactc accgtcagcc gtttcaatag ttacgccatc
      841 atcgtcatga cgaattgcgg tgaccgggca gttgaacagt tgcgcacagc ccgcttcctt
      901 cgccagttgg atccaggttt taatcgccag ttcgctgcgc aaaaaaccgg aatcagtttc
      961 aaataagccg atgtagttgt ccgggacgcg tatttccggc cagcgggcca taatcccttg
     1021 cgcatcgagc ttttcaacgt tgagttgcca ttgttcggcg ctgtgggcga cgttggcgag
     1081 aaatgtggaa tcagccgggc caaggttaat gacgccagag cgtacaaaaa tgggatcttc
     1141 ttcgttgtgg cgggagagtt catcccacag cgtttgcgcg cggaggacca gcgggacata
     1201 cttttcgcct tcaccataag catggcgaat taatcgcgta tcgccgtggt ggctgccgtg
     1261 ttgatgcggt ggcatatggg cgtcggtcat tagcacgttt aaaccggcgc gggttgcata
     1321 atacccggcg gcagcgccta cggaaccgct gccaataatg atgagatcgt atttcatttt
     1381 tttctctctg ctctcatggt ttaagcagag taattaactg aatgcagata tacaacccag
     1441 aaatggtaaa ggcaccggtg aggtgccttt tgggtggatg gtcatgtcat gttaatgacg
     1501 ccgatactcg tttacctgga aatcaccgga ttcaattttg gcgattcctg cttctaatat
     1561 cgaaataaat tgtctggcaa catcagtggt taaccagagc gtctgaccaa cttcagtccc
     1621 ttcctgctcg gacttatttg gggtctggta gtgcaaacgc aacatcagcg catcatagct
     1681 atcaacggtg ctgatgtccc accctacgag cggatgagtc tgaataactt cattattttt
     1741 ttccatcatg gccccctaat tcgtgttact agacaacgtt attcgaggtt caatgcgttt
     1801 ttatctgaag caacttcagt ataccaataa gtaagggtat tcaccgaatt tttaaagggg
     1861 gaaacagata aatttctgct tttaagattt atatgaacaa taaaacagca tgtcattcat
     1921 atttttttag catattgtgc aattattttg aggaagtgta gaaattttgt actcaaaatt
     1981 cgtaagtaaa ataaaaaagc cggggcgacc cggcaaaaaa aaatcactgc atattattcg
     2041 ctgacaaacc agtcatcggc gctttcccac gtttcctgga gaatttcact aatgcgctgt
     2101 ttatcttctt ttgtcgcgcc aataaccgat aaattattcg ctgcggcata acgtaccgat
     2161 acgtggcctt cattatcagg aaacgcatac tgaatacggc gggaaagttc gccagccagg
     2221 gcgtcaatag ccccagctgg caatggagaa gttttcgcta tggtgacttc aattcgcata
     2281 atagccccct gttgaatata ctggttattt atacaggtaa aataacctaa tgacaacagg
     2341 aagctacgat ttttattgtt taacggacca gcgtaccgtt tccccggcga ggaatggcac
     2401 cagcgtgtca tcagtcagtg cgatgctttc agcaacctgt tgctcttcac gtaccagttc
     2461 gatgaatgtg tcgttgaccg gcaacccata gaactgcggg ccgtttacag aacagaatgc
     2521 ttcaaagtgc tgcaaagcat tcatttcttc aaagacggta gcgtaactgc ccagcgcggt
     2581 tggggcgttg aagcagcccg cgcagccgca actgctctct ttgcgatgac gtgcatgtgg
     2641 cgcagaatcc gtaccgagga atactcgatt aaaaccgctg gcgaccagtt cacgcaatgc
     2701 ctgttggtga atattacgtt tgaggatggg tagacaatac aggtgcggac gcacgcctcc
     2761 aaccagcata tggttgcggt taaacatcag atgctgcgga gtgatggtgg cagccagccg
     2821 ttcatttccg tcacggacat agtcggcagc atctttggtg gtgatgtgct caaaaacgac
     2881 tttcagcgca gtcaggcgct ggcgcagagg ttccatcacg ctttctataa agcgcgcttc
     2941 acgatcaaaa atgtcgatat ctgcatgtgt cacttcacca tgcaccagta gcggcatacc
     3001 gattttttcc atgcgctcaa gtaccggcat gattgcgtca attgacgtca cgccgtggct
     3061 ggagttagtg gttgcgtttg ccgggtaaag ttttgcagcg gtgaacacgc cttcgttaaa
     3121 tccgcgctcc agctcattag gatccagcga atctgttaaa taacaggtca tcaatggggt
     3181 gaaatcgtgc ccggcaggta cggcgtcaag aatacgctgg cgatacgcca cggcagcctc
     3241 aacggtggtc acgggcggag ccagattggg cattacgata gcccgtccat aaatttcgct
     3301 ggtatatggc acgacagttt ttaacatgtc gccatcgcgg aggtgaaggt gccagtcgtc
     3361 tgggcggcgg atctttaata cctgggatgg tgcagtcatt aatctctatg ctccggctga
     3421 agggatgttt ttgccggaca caaaggataa gcggaaacgt tttcctttgc acgaaaaata
     3481 aagggcgcga atgcgccctc gtgattaatc agtaaatgga atgacaattt cgcctggctt
     3541 cacttcaatg cctttcgcca gttttttcgc cattgcttcg ccctggctgc catcttcgcg
     3601 caggacgtaa gcaggttgct ggttaaagta attgcgtaat acctggttca aatagggaag
     3661 caacgtttgc atcaccgttt gcattttttc cggttgtacc gtcgcatcga ccacttccat
     3721 ctctttcagg aagatcgcac ctttttcttt atcaaacacg ggcagcgctt tcagcttcag
     3781 tttcatggtc gctttctgac taccgaacag ggagttcatg tccagattgg cgtctccggt
     3841 tagggtaacc ttattcggct cttcgcgacc aatttggctg gtcaggtttg tcagaacaat
     3901 atgggcgtca gccacgccgg gtaaaccgat atcttttgag aaattattat gtttcgcaag
     3961 cgactggtta atttcttgtt cggtgatggt gtattgggtg agttgattac agccaacgag
     4021 caggccactg acgatcaatg cagcggcaaa taaaaacttg ttcatggtag tcctcgacat
     4081 gaaatctgcg tcaatatcct gacacaacgc agcatgtgtc accagcgata aactcgccag
     4141 cagaaaaaac tgaaaacggc ggcaacccgc gaatacaggc tgccgcggcg ggtcaggatt
     4201 aaatcgccat tgatgataac aaattgattt gtgtctgttt cgccatatta tcgcggtaat
     4261 cagcaacgcg gcttggccag ttaattccgg ctaccagcgt cagattacgc agtagcggga
     4321 atagctgaat atcatcttcc gaaagttcgc cattcacggc gttcggtttg acgatcagtt
     4381 tgtccagcgc acgtaaatca tcgctgatat tcttaatcag accgtcagag tgggccagca
     4441 ggtcggcaaa attacccgcg ctggcctctt tcttgtcgac gaaatatttg cgcgcggcgg
     4501 gagtagaaaa ctcatcaaat gccgatttgg caaaacgcgg caacagcagt ttgttggcgt
     4561 agccattgac cttgcgcagc cactcttcaa tggcagggga acgtttgccg gtcagtaacg
     4621 gtttgccgtc gagtttatcg acatagtgaa cgatgtccat gctttctggc atatagcggc
     4681 tgtcatcttt ttgcagaatg ggaacctgtt tttgaccgac catccgggtg ggtgtttctg
     4741 cgtcgtcgtt gagcagaaca tgtaattcga cggggatatt tttcaggccg aaaatcatgc
     4801 gggctttgag gcagtaaggg cagtgatcgt aaatgtatag cttcacgtga ctcctccatt
     4861 tggctgtcag ttcactttca gtatggagga gtcagggggg agtatcaaat caggcgtcgc
     4921 gttcaagcaa acgacgcgcg gcgcgtttct ggctaaactg ccaacccagc gcaaggaaag
     4981 tgaagatgcc aataatgccc agcatcatcc acggaagctc tggctggtgc gccgatttgc
     5041 ccaggtcaaa cagccagccg ccaccgatat aaccaatagc gccgccaatc gccagaccca
     5101 gacggctaaa ccccatatag ctgccgcgag ctcttgcgtc cgccagcgaa gcacttaagg
     5161 tttcacgcgc aggctcggca atgatcgacc cgatataaaa cagacaaatc agggtgaaaa
     5221 gttgttgcag gccgctgacc atgcccaccg gcatcatgct taatgacatt atcaacagcc
     5281 cagccatcaa ccggtgttcc agacgaaaat gcttttcact ccagcgggcg atagggtaga
     5341 gcaacgttaa cgacagacac gcttcaatgg catacatcca tttaacggca gagggcgcgc
     5401 cagccacgtc gttgaccata attggcagca tcagcatcac ttgtacagcc agcatgtagt
     5461 aacccgccag cgtcagaaca taggtgacaa aacgcttgtc acgcatcacg cgggtcatgc
     5521 cttcgcgaac gggcgtgcgt acggtggaga gtttccatgc tggtaacaac cacgcattga
     5581 acgccgcaca tagcacaaat agaactgccc ctgtggcgca gaccaggcga aagtcgtatt
     5641 gcaacagcca gctccccaac aatgcgccaa tgaccgcacc ggcactgtcc tgcatcatca
     5701 acagcgagaa aaaacgacca cgctgctgtg gacggattaa tttcaccacc agcgccgaac
     5761 gcggcggatc aaacaacgtg ccaccgagtc ccgagagcag gcatgaaaac cacaatagcc
     5821 acggttcgtg ggcgataccc attgtggcga atccggcggc gcgcatcagc ataccggtaa
     5881 caatcatcgg tttggcacca aagcggtcgg caattgcacc gccgaaaata cccagacctt
     5941 gctgaataaa ttggcgtaga ccgagagcaa taccgaccat gacggcggcc cagcccattt
     6001 gatcaacgaa gcggatagag atcagcggga agacaacaaa gaaccccagc acgaccagca
     6061 tattatcgat gagcaggaaa tatttaccca ggttcctcgc ctgcgacacg cgggacattt
     6121 cccctcccgg gaaataaaag atgagcactt tctattctgc attgccgcta agacttttcc
     6181 catcacttcg gggacaatat ttttttatca aaacgtcgct ttgatagaga gtttttatca
     6241 aatatgtgaa taatgcagaa aatggcattt tggacttttc acagggtctg gttgcgcagg
     6301 tatagtaata cttacagcgt attaaagacg ttacgggaag gagtaggtat agaatgtttg
     6361 gctatcgcag taacgtgcca aaagtgcgct taaccacaga ccgactggtc gtgcgtctgg
     6421 tgcatgatcg tgatgcctgg cgtcttgcgg attattacgc agagaatcgc catttcctca
     6481 agccctggga gccagtgcgc gacgaaagcc actgttatcc atcaggctgg caggccaggc
     6541 tggggatgat taacgaattt cataaacaag gttcagcttt ctactttggc ttattcgacc
     6601 cggacgaaaa agagattatt ggcgttgcca atttttccaa tgttgttcgt ggctcttttc
     6661 atgcctgcta tctcggttat tcgattgggc aaaaatggca gggcaaagga ctcatgtttg
     6721 aagccctgac cgcagccatt cgttatatgc agcgcaccca acatattcat cgcattatgg
     6781 ctaattatat gccgcacaat aaacgcagcg gtgatttact ggcgcgactg ggttttgaaa
     6841 aagaaggcta tgcgaaagac tatctgttga ttgatggaca atggcgcgat cacgtactga
     6901 cggcattaac taccccagac tggacgcccg gccgctaagg agaatctcat gaaatatcaa
     6961 cttactgccc ttgaagctcg cgtgattggt tgcttgctgg aaaagcaggt gaccacgccg
     7021 gagcaatacc cgctctcagt caatggtgta gtcacggcct gtaatcagaa aacgaaccgt
     7081 gaaccggtca tgaatctgag tgaatccgaa gtgcaggaac agctggataa tctggtcaaa
     7141 cgtcattatc tacgcacagt gagcggtttt ggtaatcggg tcaccaaata tgagcaacgt
     7201 ttttgtaatt cagaatttgg cgatctgaaa ctgagcgcag cggaagtggc gttaatcacc
     7261 acgttgttat tgcgtggtgc ccagacgcca ggtgaactgc gcagccgcgc cgcgcgaatg
     7321 tatgaattca gcgatatggc ggaagtggag tcgacgctgg aacaactggc aaatcgcgaa
     7381 gatggtcctt ttgtggtgcg tctggcccgc gaaccgggta aacgcgaaaa ccgctacatg
     7441 catcttttca gtggtgaggt tgaagatcag ccggcggtga cggatatgtc gaacgcggtt
     7501 gacggtgatt tacaggcccg cgtcgaagcc ctggaaatcg aagtggcaga actgaaacag
     7561 cgtcttgatt cgttgctggc ccatctggga gattaaagtg aaaaaattac gtatcggcgt
     7621 agtgggatta ggtggcattg cgcaaaaagc gtggttaccg gtgctggcgg cagcgtctga
     7681 ctggacgtta caaggagcct ggtcgcctac gcgcgcgaaa gccctgccaa tttgtgaaag
     7741 ctggcgcatt ccttatgccg attcgttatc cagccttgcc gccagttgcg atgcggtttt
     7801 tgtgcattcc agcaccgcca gccactttga cgtggtcagt acgttactca atgcaggggt
     7861 acatgtctgt gtcgataaac cgctggcaga aaatctgcgc gatgctgaac ggctggtgga
     7921 actggcggcg cggaaaaaac tgacgttgat ggtcggtttt aaccgtcgtt tcgcaccact
     7981 ctacggtgag ttaaaaacgc aactcgccac cgcagcctcg ctaagaatgg ataaacatcg
     8041 tagcaatagc gtcgggccac acgatcttta tttcacgttg ctggatgatt atctgcatgt
     8101 ggtggatacc gcgctgtggt tgtcgggcgg caaagcctct ctggatggcg gtacgctact
     8161 gactaacgac gctggcgaaa tgctgtttgc cgagcaccat ttttcggctg gtcctttgca
     8221 gatcaccacc tgtatgcatc gccgtgccgg aagtcagcgt gaaaccgtgc aggccgtgac
     8281 tgacggtgcg ctcatcgaca ttacggatat gcgcgaatgg cgtgaggagc gcgggcaggg
     8341 cgtagtgcat aaaccgattc ctggttggca gagtacgctt gagcaacgtg ggtttgtcgg
     8401 ctgtgcgcgg cacttcattg aatgtgtgca aaaccagaca gttccgcaaa ccgccggcga
     8461 acaggccgtg ctggcgcaac gtatcgttga caagatctgg cgcgatgcga tgagtgaata
     8521 aaccctgtaa catctggcgg tagcaattca ccataatcca ggtagactat tcgcctcttt
     8581 cagcgcctgc cttgcaggcg ttttgcccgt gggtctggat tagaacaccg atgaatttat
     8641 taaaatcgct ggccgccgtc agctcgatga ccatgttttc gcgtgtgctt ggcttcgcac
     8701 gagacgcaat tgtcgccaga atctttggcg cagggatggc aaccgacgcc tttttcgtcg
     8761 cttttaaact tcctaacttg ttacgccgta tctttgccga aggggcattt tcccaggcat
     8821 ttgtaccgat tctggcggaa tataaaagta agcagggtga agacgccacg cgggtctttg
     8881 tctcttatgt ttctggcctg ctgacacttg cgctggcggt tgtgacggtc gctggcatgc
     8941 tcgccgcacc gtgggtgatc atggtgaccg cgccaggctt cgctgacaca gctgacaaat
     9001 ttgccctgac cagccagcta ctaaagatta cctttcccta tatcttgctg atctccctgg
     9061 cgtcgctggt gggagcgatt ctgaatacgt ggaaccgctt ctcgattccg gcgtttgctc
     9121 caacactgct taacatcagc atgattggtt tcgcgctgtt tgccgcaccg tactttaacc
     9181 caccggtgct ggcgctggcg tgggctgtta cggtcggcgg cgtcctgcag ctggtgtatc
     9241 agctaccgca cctgaagaag atcggcatgc tggtcctgcc gcgcattaac ttccacgatg
     9301 ccggagcaat gcgcgtggtg aaacagatgg gaccggcgat ccttggcgtc tctgtgagcc
     9361 agatctcctt aatcatcaac accatttttg cctcgtttct tgcttccggt tcggtgtctt
     9421 ggatgtatta cgccgaccgc ttaatggagt ttccgtccgg tgtgctgggc gtggcgcttg
     9481 gcaccatttt gctgccgtcg ctgtcgaaaa gttttgccag tggcaatcat gatgaataca
     9541 accgtttgat ggactggggg ttgcgtcttt gtttcctgtt ggcgctgccg agtgcggttg
     9601 cgttgggcat tctttccggt ccgttgaccg tttcgctgtt ccagtacggt aaatttaccg
     9661 cgtttgatgc gctgatgacc cagcgggcgt taattgccta ctcggtgggt ttgattggcc
     9721 tgattgtagt gaaagtgttg gctcctggct tttattcccg ccaggacatt aaaacgccag
     9781 tgaaaattgc catcgttacg ctgattttaa cgcaattgat gaacctggcg tttattggtc
     9841 cgttgaaaca tgccgggctg tcactttcta ttggtctggc ggcgtgtctg aatgcttcgc
     9901 tgctttactg gcagttgcgt aagcagaaaa tctttacccc gcaacccggc tggatggcgt
     9961 ttctgttgcg tctggtggtg gcggtactgg tgatgtctgg cgtgctttta ggtatgttac
    10021 atatcatgcc ggagtggtca ttgggtacca tgccctggcg tttactgcgt ttaatggcgg
    10081 tcgtgctggc ggggattgcc gcgtacttcg ctgcactggc ggtactgggc ttcaaagtta
    10141 aagaatttgc ccgccggacg gtgtaacaat gcattccggc ctgcagtgca ggccggagat
    10201 aatcttcaga tcgaaatctt tttaccgccg cgatgggttg ttgaggtctg accgttcgcc
    10261 ccatatagcg tcggttcctg atgcggtttc aacatttcca gcgcctgttg attgcgctca
    10321 atctgtcctt ccagtaacca gccgttatgt tgattcattt ggcgtagttg ctgcgttttc
    10381 acagtaattt cctgccagcg ttgactaata tcaacgctat ttgctgtatt gggttctttc
    10441 ctgcgtaact gttcgaggta atccagcgtc gccagcagtg agcttttttg ttctgtaatc
    10501 cattgcaact ggctgccgtt gatctgcccc atagagagat gttgctgctc ttgatccatt
    10561 accgttttga gatcgttaag cacagcggac atctggtcga ggatctctgc aagacgtgtc
    10621 atacgatcag ttactctgca agtcttgctg cgcttcgttg atcagcgcat cggcaatttt
    10681 gccggtgtcc atttttagtt caccgttacg aatcgccagt tttaacgctt cgacacgttc
    10741 aagattgata tcactgctgc cgggttgcat cagttttgct tgcgcgtcgc ttaacgtcac
    10801 actggtgctg gtggaggcgg ttgtttttgc cgcccggctg ttcgttaccg gcgcgtcagt
    10861 ggtttcgcgc ggttgaacgg tgcttacagg cttcagaggc gaagtgcgat caatactcat
    10921 ggtttattcc tcattgaggg cgcttttatc atgtgttgct tatttatcgg caagggacgg
    10981 gtaatcttta acagcttaca ggtttataag aatattccca tctgcatcaa caacgccgct
    11041 gactacctgt cccgatacca tgcgcacccg cgcattctgt gcgacggctg cattgttcag
    11101 cgcctgacct tctgcgttgg cgctaaaccc atcaccgctg gcgatcacat tgacgcgttg
    11161 tcccgctttt acccgccatg cctggcgaaa ctgggttaac tggataggtt gatcgggtga
    11221 tagatcgcgc aggctaatgg catcaacaag ttgattgata tccagcaccg tacgcggtgg
    11281 cagggtatcc agccgtccgc gtttcagttt gacattgcca gcttccagct ttcctccccg
    11341 cgcaatgggc atcgcggcaa ccacataatt tcctgtggcc tgtacattaa cctgtaaata
    11401 tcgtttgtcg ttaccgcagc gtgccaacac attcacattg ccccacaggc gggaattatt
    11461 gctcatcgaa agcaatggct gctcgcatgg cggtagtaga ttgggcgctg tacgaataga
    11521 aacacgaacc tcatcactta cccccgcgag ttgggcgcta aaaaagttgt gcaattgcga
    11581 tgtgagattg ctcgccgtac ttaacggact gaacagtatc gcgatgatcg ccacgctacg
    11641 ttttattatc agcattttcg cccccagcca tttctacaac gtgaattgta cctgtccgca
    11701 atgaccatca acggcataaa tagcgaccca ttttgcgttt attccgccga taacgcgcgc
    11761 gtaaaggcat ttaagctgat ggcagaattt tgatacctgc ggaggagata tgctcgataa
    11821 gctcgacgcc gccttacgtt ttcaacaaga ggcgctcaat ctgcgcgccc agcgtcagga
    11881 agtgctggca gcaaacatcg ccaatgccga tacccctggt tatcaggcgc gcgatatcga
    11941 ttttgccagt gaacttaaaa aagtcatgca acgtggacgg gatgcaacca gtgtggttgc
    12001 actgacgatg acctcaacgc aacacattcc ggcgcaggcg ctgacgcctc ctaccgcaga
    12061 actgcaatac cgtattccgg accagccttc gcttgacggt aataccgtcg atatggatcg
    12121 cgaacgcacc cagtttgccg ataacagcct gcaataccag atgagcctta gcgcgttgag
    12181 cgggcaaatc aaaggcatga tgaacgtttt acagagcgga aattaacgga tggcactgct
    12241 gaatattttt gatatcgccg ggtcggcgtt aactgcccag tcccagcgcc tgaacgtggc
    12301 ggccagtaat ctggcgaatg ctgatagcgt gaccggtccc gatggacagc catatcgggc
    12361 aaaacaggtg gtattccagg ttaacgctgc accaggtgct gcgacaggcg gcgtaaaggt
    12421 tgccgatgtt atagaaagtc aggccccgga caaactggtt tatgaaccgg gtaatccgct
    12481 ggcagatgca aagggctacg taaaaatgcc gaacgttgat gttgtcggag agatggttaa
    12541 caccatgtcg gcgtcacgca gctatcaggc caatgttgaa gtgctcaaca cggtgaaaag
    12601 catgatgctg aaaaccctta cgctcggtca ataaaggaga aagctatgtc cattgcggta
    12661 accaccaccg atccgacaaa taccggcgtc agtaccacca gcagtagttc gctcacgggc
    12721 agcaacgccg cagatttaca aagcagtttt ctgactttgc tggtggcgca gctgaaaaac
    12781 caggacccga ccaatccaat ggaaaacaac gagctgacgt cgcaattggc acaaatcagc
    12841 acggtcagcg ggattgaaaa actcaatacc acgctcggat ctatttccgg acagattgat
    12901 aacagccagt cgttacaggc cagtaacctg atcggtcacg gcgtgatgat ccccggcacc
    12961 actgttcttg cgggaaccgg cagtgaagaa ggggctgtga ccacgaccac gccgtttggt
    13021 gttgagctgc aacaggcggc agacaaagtt acggccacca tcaccgataa aaatggcgcg
    13081 gttgtgcgca ccattgatat tggtgaactg accgccggag ttcacagttt cacctgggac
    13141 ggtacgttga ctgatggcag cactgcgccg aacggttctt acaatgtagc gattagcgcc
    13201 agtaacggtg gtacacaact ggttgcccag ccgctgcagt ttgctctggt gcagggtgtg
    13261 atccgcggca acagcggtaa tacgctggat ctcggcactt acggcaccac caccctcgac
    13321 gaagtacggc agataattta agccttcaca tttcaggagt cagtcatggc cttttctcaa
    13381 gcggttagcg gattaaacgc tgccgccacc aacctcgatg ttattggcaa caatatcgcc
    13441 aactccgcca cctacggctt taaatcaggc acggcctctt ttgccgatat gtttgccggt
    13501 tcgaaagtgg gactgggggt aaaagttgcc ggtatcactc aggactttac cgatggcacg
    13561 accaccaaca ccgggcgagg tctggacgtt gctatcagcc agaacggttt tttccgtctg
    13621 gtagacagca acggttcggt gttctacagc cgtaacggac aatttaagct ggatgaaaac
    13681 cgtaacctgg tgaatatgca aggtttacag ctgacgggtt acccggcaac cggtacgccg
    13741 ccgactattc agcaaggggc gaatccgacc aatatttcga tcccgaatac cctgatggca
    13801 gcgaaaacta ccaccacggc atcgatgcag atcaacctga attccagtga tccgcttcct
    13861 actgttacgc cattcagcgc cagcaatgcg gatagctata acaaaaaagg ttcggtgact
    13921 gttttcgaca gtcagggtaa tgctcatgac atgagcgtct actttgtgaa gaccggggat
    13981 aataactggc aggtctacac ccaggatagc agtgatccaa acagcattgc gaagacagcg
    14041 acaacactgg aatttaatgc taatggcaca ttagtggatg gtgcgatggc gaataatatc
    14101 gcaaccggcg caattaacgg tgcagaaccc gccacgttta gtctgagctt cctcaactcc
    14161 atgcagcaaa ataccggcgc taacaatatt gtggcaacca cccagaacgg ctacaaaccg
    14221 ggcgatctgg tgagttatca aatcaatgat gacggtacgg ttgtcggcaa ctattccaac
    14281 gaacaaaccc aactgctggg gcagattgta ctggcgaact ttgccaacaa cgaaggtctg
    14341 gcatccgaag gcgacaacgt ctggtctgcg acgcaatctt ctggcgtggc gctgttgggg
    14401 acagccggga cgggaaactt tggcaccctg accaacggtg cgctggaagc gtccaacgtc
    14461 gatctcagta aagaactggt caatatgatc gttgcccagc gtaactatca gtctaacgcc
    14521 cagaccatca aaacccagga ccagatcctc aacacgctgg ttaacttacg ctaatcgctg
    14581 acgggatagc tcaatggatc acgcaattta taccgcgatg ggagcagcca gccagacact
    14641 gaatcaacag gcggtaaccg ccagtaatct ggccaatgcc tcaacgccag gttttcgcgc
    14701 gcagttgaat gctttacgcg cggtgccagt ggaagggctt tctctgccca cgcgcacgtt
    14761 ggtcacggcg tcaacgccgg gcgcagatat gacgcccggc aaaatggatt acacctcgcg
    14821 cccgctggac gtcgcgttgc agcaggatgg ctggccgtgc agaccgctga cggcagcgaa
    14881 gggtatacgc gtaatggcag cattcaggtt gatcccaccg ggcaactgac aattcagggg
    14941 catccggtga taggcgaggc tgggccaatt gctgtgccgg aaggggcgga aatcactatt
    15001 gctgccgatg gcacaatctc ggcgctcaat ccgggcgatc cggcaaatac ggttgcgcca
    15061 gtagggcgtc ttaaactggt gaaagccacg ggcagcgaag tgcagcgcgg tgacgacggc
    15121 atttttcgtt taagcgcaga aacccaggcc acgcgtgggc cggtactgca ggcagatcca
    15181 accttgcgtg tgatgtcggg ggttctggaa ggcagtaacg tcaatgccgt tgcggcaatg
    15241 agcgacatga ttgccagcgc gcggcgtttt gaaatgcaga tgaaggtgat aagcagcgtc
    15301 gatgataacg caggccgtgc caaccaactg ctgtcgatga gttaattgaa aggatacatg
    15361 acaagtataa gttgcccgat gcgcaagttt atcgggtcta tgggggcaat cgcaatttat
    15421 cgattttgcg agcacttgta ggccggataa ggcgtttacg ccgcatccgg caagaagaca
    15481 tatgcacttt gtcactaatc cactacagga cattttatga tcagttcatt atggatcgcc
    15541 aaaacgggcc ttgacgccca gcaaaccaat atggacgtca ttgccaacaa cctggcaaac
    15601 gtcagtacta acggttttaa gcgtcagcgc gcggtgtttg aagatctgct ttatcaaacc
    15661 attcgccagc cgggggcaca gtcttccgaa caaaccacct taccctccgg attacaaatc
    15721 ggcacggggg tacgcccggt cgccactgaa cgcttacaca gccagggaaa cctgtcgcag
    15781 accaacaaca gcaaagatgt cgcgattaaa gggcagggct ttttccaggt gatgttgcca
    15841 gatggttcat cagcctatac ccgtgacggc tctttccagg tggatcagaa cgggcagctg
    15901 gtgacggctg gtggttttca ggtgcagcca gcgatcacca ttccggcgaa tgcgttaagt
    15961 atcaccatcg gtcgtgatgg cgtggtcagc gtaacccaac aaggccaggc agctccggtt
    16021 caggttgggc agctcaatct caccaccttt atgaatgaca ccgggctgga gagcattggc
    16081 gaaaacctct acaccgaaac gcaatcctct ggtgcaccga acgaaagcac gccgggcctg
    16141 aacggcgcgg gactgctgta tcaagggtat gttgaaacgt ctaacgtcaa cgtggcggaa
    16201 gaactggtca atatgattca ggtgcaacgc gcttacgaaa tcaacagtaa agcggtgtcc
    16261 accaccgatc agatgctgca aaaactgacg caactctaag gcttaaccgg tggcaggttc
    16321 accggtttac tgatttttga agatgatagc catgcaaaaa aacgctgcgc atacttatgc
    16381 catttccagc ttgttggtgc tttcactaac cggctgcgcc tggataccct ccacgccgct
    16441 ggtgcagggg gcgaccagtg cacaaccggt tcccggtccg acgcccgtcg ccaacggttc
    16501 tattttccag tctgctcagc cgattaacta tggctatcaa ccgctgtttg aagatcgtcg
    16561 accacgcaat attggcgata cgctgaccat cgtgttgcag gagaacgtca gcgccagcaa
    16621 aagctcctct gcgaatgcca gccgtgacgg taaaactaat tttggctttg atactgtgcc
    16681 gcgctatttg caggggctgt ttggtaacgc tcgtgccgat gtcgaagcct ccggtggtaa
    16741 cacgttcaac ggaaagggcg gggccaatgc cagcaatacc tttagcggca cgttgacggt
    16801 gacggttgac caggtactgg tcaacggcaa cctgcatgtg gtgggtgaaa aacagattgc
    16861 cattaatcag ggtaccgaat ttattcgctt ctctggcgtg gttaatccac gcactatcag
    16921 cggcagcaat accgtaccgt ctactcaggt ggcggatgcg cgcattgaat acgtaggcaa
    16981 tggctacatt aacgaagcgc aaaatatggg ctggttgcag cgtttcttcc ttaacctgtc
    17041 gccaatgtaa gtgaggctgt ggtgattaaa tttctctctg cattaattct tctactggtc
    17101 acgacggcgg ctcaggctga gcgtattcgc gatctcacca gtgttcaggg ggtaaggcaa
    17161 aactcactga ttggctatgg tctggtggtg gggctggatg gcaccggtga ccagacaacc
    17221 cagacgccgt ttaccacaca aacgcttaat aacatgctct cacagctggg aattaccgtt
    17281 ccgacgggca ccaatatgca gctaaaaaac gtcgctgcgg taatggtgac agcgtcactt
    17341 cctccgtttg gacgtcaggg gcaaaccatc gatgtggtgg tttcttccat gggaaatgcc
    17401 aaaagcttgc gtggaggtac gttgttgatg acaccgctta agggcgttga cagtcaggtg
    17461 tatgcgctgg cgcagggcaa tattctggtt ggcggcgcag gagcctccgc tggcggtagc
    17521 agtgttcagg ttaaccaact gaacggtgga cggatcacca atggtgcggt tattgaacgt
    17581 gaattgccca gccagtttgg cgtcgggaat acccttaatt tgcaacttaa cgacgaagat
    17641 ttcagcatgg cgcagcaaat cgctgacacc atcaaccgcg tgcgtggata tggcagcgcc
    17701 accgcgttag atgcgcggac tattcaggtg cgcgtaccga gtggcaacag ttcccaggtc
    17761 cgcttccttg ccgatattca gaatatgcag gttaatgtca ccccgcagga cgctaaagta
    17821 gtgattaact cgcgcaccgg ttcggtggtg atgaatcgcg aagtgaccct cgacagctgc
    17881 gcggtagcgc aggggaatct ctcagtaaca gttaatcgtc aggccaatgt cagccagcca
    17941 gatacaccgt ttggtggtgg acagactgtg gttactccac aaacgcagat cgatttacgc
    18001 cagagcggcg gttcgctgca aagcgtacgt tccagcgcca gcctcaataa cgtggtgcgc
    18061 gcgctcaatg cgctgggcgc tacgccgatg gatctgatgt ccatactgca atcaatgcaa
    18121 agtgcgggat gtctgcgggc aaaactggaa atcatctgat gatcagcgac agcaaactac
    18181 tggcaagtgc ggcctgggat gcgcaatcac tcaacgaact aaaggcgaaa gcgggcgaag
    18241 atccggcggc aaatatccgt ccggtggccc gtcaggtgga agggatgttc gtgcagatga
    18301 tgttgaaaag catgcgcgac gctttaccaa aagatggcct gttcagcagc gagcacactc
    18361 gcctgtatac cagtatgtat gaccagcaga ttgcccaaca gatgacggcg ggcaaaggtc
    18421 tggggcttgc agagatgatg gttaaacaga tgacgccaga acaaccattg ccagaggagt
    18481 ccacgccagc agcaccgatg aaattcccgc tcgaaactgt ggtgcgttat caaaatcagg
    18541 cgctttcgca gctggtgcaa aaggccgtgc cacgtaacta cgatgattcg ctgccgggtg
    18601 acagtaaagc attcctcgcg caactctcgc tgcccgccca actggcaagc cagcaaagcg
    18661 gtgtgccaca tcatttgatc ctcgctcagg cggcactgga atctggttgg gggcaacggc
    18721 aaatccgccg cgaaaacggc gagccgagct ataacctgtt tggtgtcaaa gcctctggca
    18781 actggaaagg gccagttact gaaatcacca cgactgaata tgaaaacggc gaagcgaaga
    18841 aagtaaaagc gaagtttcgc gtctacagct cgtatctgga agccttgtcg gattacgttg
    18901 ggctgttaac gcgtaacccg cgctacgccg ccgtgacgac cgccgcgagt gcggaacagg
    18961 gggcgcaggc cctacaggac gcgggctatg ccaccgatcc tcactatgcc cgcaaactca
    19021 ccaacatgat tcagcagatg aaatcgataa gcgacaaggt gagcaaaacc tacagtatga
    19081 acattgataa tctgttctga ataactcaag tccggcgggt cgctgccgat aatactctgt
    19141 aattgaaggc ttataaggaa cctccatgtc cagcttgatt aataacgcca tgagcggact
    19201 gaacgcggcc caggcggcgt taaatacggc aagtaataat atctccagct ataacgttgc
    19261 cggatatacc cgccaaacca ctattatggc gcaggccaat agcacgttgg gcgctggcgg
    19321 ctgggttggc aatggtgtct acgtttctgg tgtgcagcgt gagtatgatg cgtttattac
    19381 caaccagtta cgtgcggcgc agacgcaaag tagcggtctg actgcccgct atgagcagat
    19441 gtcgaaaatc gacaatatgc tctccaccag tacctcttcg ctggcaacac agatgcagga
    19501 tttcttcacc agcctgcaaa cgctggtgag taacgcggaa gacccggcag cgcgccaggc
    19561 gctgattggg aaatcagaag gattggtgaa tcagtttaaa accaccgatc aatatctgcg
    19621 cgaccaggac aaacaggtca atatcgcgat aggtgccagc gttgatcaga tcaacaacta
    19681 cgctaaacaa attgccagcc tgaacgatca aatctcgcgc ctgacaggcg tgggggcagg
    19741 ggcgtcacct aacaatctgc tggatcaacg cgatcaactg gtgagcgaat taaaccagat
    19801 tgttggtgta gaagtcagcg ttcaggatgg cggcacttat aacatcacga tggccaatgg
    19861 ttactcactg gttcagggaa gtacggcgcg gcaactggcg gcagttcctt ccagcgctga
    19921 cccttctcgt acgactgtcg cttatgttga tgggacggca ggcaatattg agatcccgga
    19981 gaaattactg aataccgggt cgctgggcgg cattctgaca ttccgttctc aggatctgga
    20041 ccagacgcgt aatacgcttg gacaactggc gctggcattt gccgaggctt tcaacaccca
    20101 acacaaagcc ggatttgatg ctaacggcga tgccggtgaa gatttctttg ctatcggtaa
    20161 gcccgcggtt ctgcaaaaca cgaaaaacaa aggtgacgtt gcgatcggtg ccacggtaac
    20221 tgatgcctcc gcggtactgg cgacagatta caaaatctcg ttcgataata atcagtggca
    20281 ggtcacccgc cttgccagca ataccacttt tacggtgacg ccggatgcca acggtaaagt
    20341 ggcatttgat ggtctggagt tgacgtttac aggaacgcct gccgttaacg acagcttcac
    20401 gctgaaacca gtaagtgacg ccatcgtcaa catggatgta ttaatcaccg acgaagcgaa
    20461 aatagcgatg gcgagcgaag aagatgcggg tgatagcgat aaccgcaacg gtcaggccct
    20521 gctggatctg caaagcaaca gtaaaacggt gggcggtgcg aaatccttta acgacgctta
    20581 tgcctcgtta gtgagtgata tcggtaataa aaccgcgacg ttgaaaacca gtagcgccac
    20641 gcaaggtaat gtggtgacgc agctttccaa tcagcagcag tcgatttccg gtgtcaatct
    20701 cgatgaggag tacggaaatc tgcaacgttt tcagcagtat tacctggcga atgcgcaggt
    20761 tctgcagacg gcaaacgcga tttttgatgc gctgattaac attcgctaag gggagataag
    20821 atgcgtttca gtacacagat gatgtaccag caaaacatgc gtggtatcac caattctcag
    20881 gcagaatgga tgaagtacgg cgaacagatg tcgacgggta agcgagtcgt taacccttct
    20941 gacgatccca ttgctgcatc acaagccgta gttctctccc aggcacaggc gcaaaacagc
    21001 cagtacacgc tggcgcgtac tttcgccact caaaaagtgt cactggaaga gagtgtactt
    21061 agccaggtca ccactgctat ccagaatgct caggaaaaaa ttgtctacgc cagcaatggc
    21121 accttgagtg acgatgaccg ggcctcgctg gctacggata ttcaggggct tcgtgaccag
    21181 ttgctgaatc tggcaaacac cactgacggt aacgggcgct acatttttgc cggttataaa
    21241 acagagactg cgccgtttag cgaagagaaa gggaaatacg tcggtggagc agaaagtatt
    21301 aaacaacagg tcgatgcttc gcgttcgatg gtgatagggc acacgggtga caaaattttc
    21361 gacagtatta ccagcaacgc ggtagcggaa ccagacggta gcgcttctga aaccaatctt
    21421 tttgccatgc tggatagtgc catcgcagcc ctgaaaacgc cggtcgcgga tagcgaagcg
    21481 gataaagaaa ccgccgctgc ggcgttagat aaaaccaacc gcggactgaa aaactcgctg
    21541 aacaatgtgc tgactgttcg cgcggaatta ggcacgcagc tgaacgaact ggagtcgctg
    21601 gattcattag gtagcgatcg cgctttaggg caaacgcagc agatgagcga tctggttgat
    21661 gtggactgga atgcaactat ttcatcttac atcatgcagc aaacggcatt gcaggcatcg
    21721 tataaagcat ttaccgatat gcagggattg tcgctcttcc agctcagcaa ataatttcgc
    21781 tttaaaacat atcatgaaac tgggtatgtt ttgtctgcct gctctgggat cgctggggcg
    21841 ggcatttttt tgcctatttt gcattgttgg ttagcaagga tgccattcga tgaattttaa
    21901 tatgttgatt caaagatgaa ataaaaaagc cctggcagtt accagggctt gattactttg
    21961 agctaattat tactcaacag gttgcggacg cgcaggagcg gcagaggcat gatgtgttgc
    22021 cgtatgacca cctgcggcac ctttaccttc gaaggcaaaa gtagggcgct gccagtcact
    22081 gtgacgcggt gcctccggaa catattccgg tgctggagcg cgcgtcattg gcgcggtagc
    22141 gtggttatgc tcaacggtga cctcaggttc gaccgcagct accgtttcaa cttctgctgc
    22201 gacttcagcg actactggcg cggcaggttg agcaacaact tcaggttcag caactacaac
    22261 ctcagcagtt tcgacaactt cttcaatatc tgccgtctct tcctgcggtt caaccaccgg
    22321 ttctgcttgt tctgcaactt cctgggctac ggcaacatca gactcggtaa tcacctgcgg
    22381 ctgttcagtt accgcggcag cgatcacttc aggatgcgtc gtttcaacca cttccggttg
    22441 cggttcggca acctgaacgg gggcttctac gacaccggcc acttcttcaa caactggcgc
    22501 gctaacaacc ggttcgatag cggcggcgac agggacctca gtcaccatcg gctgcacatg
    22561 tacttcttcc tgttcgcgct gctcttcaac ctgtacatct tgcggacgta caattggata
    22621 gcggatccag actttgccag aggccagttc cggagacgcg cacgctacgg tcaacggcat
    22681 tggcgactgg gttggataac gctcgtcacg atagcgacga cgacgctgac cacttacgcg
    22741 caggtgacga ggcgagcggc gagaacgacg cggcatgcca ccgttgtcac ggttatcagc
    22801 attgttctct tcttgctgtt ctggtgcagt ttgcgctacg actggcagcg ggactttcac
    22861 cagttctgtg cgtggagctg gcgcttcctg aacaattggt tcggcagcga cagtttcttc
    22921 aaccaccggt gcgactaccg cttcttcggc tacgctttgc tcgtaacgca ctttctgatt
    22981 gagctgacgc tgtttacgac gcggctgaac cggacgtaca cgttcttcct gttcggtttc
    23041 ctgaacagat tgctcttcaa cattcagcgc cttcgcttct tgttgcgcct gacgtttatc
    23101 atcattacgg cggcggctac gttcacgacg cggcgcttgc tgctcgtcgg cggtacgcgc
    23161 tttttccgtt acctcagcct gctgacggct ctcacgcgtc tcggcagtct gctgctgtgc
    23221 ctggcgacga ttacgacggt tttcttcgcg attatcgctg ccttcagtac gttcactacg
    23281 ggtgtcgcgg cgctcattac ggtcacggcg gttgttctga cgaggcttgc gacgatcctg
    23341 ttgacgttcc ggtttcgctt ctgctttcgg tgctggttgc tcggtcggtt tggtttcttc
    23401 accaccgctg aacagcgctt tcagtgcgcc gaagaagcgg ctcaacagcc caggttgtgc
    23461 aggagctgct ggtgttgccg gtgcagcttt cggtgctgga gctacaacag gcgcggcagg
    23521 ttcagctggc gttggcgcag gcggcacatc cggcatggca aaggttgcca gcgcaggttg
    23581 ttccggacgc ttacgttcag cgaactcttc ttcagacggc agcgccatcg cttcttcatg
    23641 cagcttcggc agcatgtagc ttaaggttgg ggtttcttcc cctttacgca cgcgcagcac
    23701 gtggtagtgc ggggtttcca tctgatcgtt tggcacaatt acacagcgca caccgtcctg
    23761 acgagtttca atggcattta ccgcagaacg tttttcattc agcaggtaag aagcgattgg
    23821 cacaggaaca atggcgtgaa cttcctgggt gttctctttc agcgcttctt cttcgatcag
    23881 acgcagaata gagagcgaca gcgattcgtt gtcacgcacg gtgccagtac cagaacaacg
    23941 cggacaaacg tgatgactgg attcacccag tgatgggctc aggcgctgac gggacatttc
    24001 cagcaggcca aagcgagaaa tatggctgat ttgaatacgc gcacggtcct gacgcaccgc
    24061 ttcacgcaga cggttttcta ccgcacgctg gtggcgtact ggcgtcatgt cgatgaagtc
    24121 gataacaatc aggccgccga ggtcacgcag gcgcagctga cgagcaatct catcggcagc
    24181 ttcgaggtta gtgttaaacg cggtttcttc gatatcgccg ccgcgggtcg cgcgtgcgga
    24241 gttgatgtcg atggccgtta acgcttcggt gctgtcgata acaatggaac caccagacgg
    24301 cagacgaact tcacgctgga aggcggactc gatctgtgac tcgatctggt agtggctgaa
    24361 cagcgggatc tcgccggtgt acagtttgat tttgctgctg aaatccgggc gacctaatgc
    24421 agcgatatgc tgacgtgcca gttcgagcac tttcgggtta tcgataagga tttcgccgat
    24481 gtcctgacgt aagtaatcgc ggaatgcgcg aacgattacg ttgctctcct gatgaatcag
    24541 gaacggggcc gggcggcttt cagcggcttt tttgatggct tcccagtgtt tcagacggaa
    24601 gcttaaatcc cattgcagcg cctcagcaga tttgccgacg ccagcggtgc gcacgataag
    24661 ccccatgcct tccggcagtt caaggcttgc cagtgcttct tttaattcgg tacggtcgtc
    24721 gccttcgata cggcgagaaa tgccacccgc gcgcgggttg ttcggcatca gaaccagata
    24781 gctacccgcc agactgataa aggtggttaa tgccgcgcct ttgttgccgc gctcttcttt
    24841 atcgatctga acaatgactt cctgaccttc acgcaacaca tctttaatgt tgggacgacc
    24901 atgagcactg tagttagcag ggaaatattc gcgggcaatt tcttttagtg ggaggaaacc
    24961 gtgacgttca gcgccgtaat caacaaaagc agcttccaga ctcggttcaa tgcgggtgat
    25021 tttacctttg tagatgtttg cctttttctg ctcgtgccct ggactttcga tatccaggtc
    25081 atacagacgc tgcccatcta caagggcaac gcgcaactct tcctgctgag ttgcgttgat
    25141 taacattctt ttcatcgtaa cttactcatt attcttacat tgacgactaa gctgcgggca
    25201 aagtaacgcc tttccgggtg tgaaccgatg gcctcgtgtc tagtcgcgtc gccaacctca
    25261 cggttatcgt cagctcaaag aggcgcagag tgtcggttgc ccgtttttca tgcggaaaaa
    25321 cagcgcaatt atcaaagaaa cagactgggt attactctcc agaaatattt ccatctaccg
    25381 gtaaggactg caacccgcag cccgctaact gcctgaaaga tcaatacgtc ttacgccatt
    25441 gctgcgtgga tgatcggtcg ggcaaaatgg gttattccgt aaaatttctt gttttaacaa
    25501 ggatggacac ggaaacggcc tcattattcc actgcaagcc ttgttatagc aagatgactt
    25561 ttaccattta tcacccgctt actcacagtt ttttcacttc ttgctggtga ttggtttaat
    25621 aaccatcaat aaagtgatca cgcagtgaag taataaggtg caaaagtaaa tataagcata
    25681 gaaaaatgag tggcgcgaat cctgttggct aattagaatc gccaaccatg aaaacagaga
    25741 ctccatccgt aaaaattgtt gctatcaccg ctgacgaagc ggggcaacgt atcgataact
    25801 ttttgcgtac ccaattgaaa ggcgtaccaa aaagtatgat ttaccgtatt ttgcgtaaag
    25861 gcgaagtgcg ggtgaacaaa aaacgtatta agcctgaata taaactcgaa gcgggtgatg
    25921 aggtgcgtat tccaccggtt cgcgttgctg agcgggaaga agaggcggtt tcgccacatc
    25981 tgcaaaaggt ggcggcgctg gcggacgtca tcttatatga agatgatcac atcctggtgc
    26041 tgaataaacc ttccggtacg gcggtacatg gcggcagtgg tttaagcttc ggcgttattg
    26101 aaggtttgcg ggcgttgcgc ccggaagcgc ggttccttga actggttcat cgtcttgacc
    26161 gggacacctc aggtgttttg ctggtagcga aaaaacgctc ggcgttgcgt tctctgcatg
    26221 agcaattacg tgaaaaaggg atgcaaaaag attacctggc gctggtgcgc ggtcagtggc
    26281 agtcgcatgt gaagagcgtt caagcaccgt tactgaaaaa tattctgcaa agcggcgaac
    26341 gtatcgtgcg tgtgagtcag gaaggcaaac cgtcggaaac acgctttaaa gtggaagaac
    26401 gctatgcatt tgccaccctg gtgcgttgta gcccggtaac agggcgtact caccagatcc
    26461 gtgtgcatac acaatatgcg ggtcatccga ttgcctttga cgatcgctac ggtgaccgtg
    26521 aatttgacag gcagctcact gaagcaggca cgggattaaa tcgcctgttc ctgcacgctg
    26581 cagcgttgaa gtttactcat ccggggaccg gtgaggtgat gcgtatcgaa gctccgatgg
    26641 atgaaggttt gaagcgttgt ttgcaaaagc tgcgtaacgc gcgctaatac aagcatataa
    26701 acctgataat gggcggttgg cctgataagg cgtttacgcc gcatcaggcc gccagcaccg
    26761 attgccggat gcgacgtaac cacatccggc gcaaaaaact atcccatcag cgggtttttc
    26821 ccttcccgac gtaacatctg acacagcgcg attagcggta aaccaaccag cgtgttagga
    26881 tcacgcccct ctaaacgctc aaacagcgta atgccaaatc cttcactctt aaagctaccc
    26941 gcgcagtgca ggggatgctc tttacgcacg taattatcaa tctccgcctc gctcaggtga
    27001 cggaaatgga cgtcaaaagg ctccacttct gtttgcagat gcccattcgc cgaattaaac
    27061 aacgccagtc cagtatagaa ggtgacgata ttgccgctgg ctttgcgtaa ttgcagacgg
    27121 gcattttctt ccgttaacgg tttaccggtg atttcgccat caagaacaca tacctggtct
    27181 gaaccaataa ttaaatgatc cggataacgt gacgccagag attgcgcttt ttcttgtgcc
    27241 agtcgaagca ccaactgtcg cggtgattcg tcgctgcgtg gggtttcgtc gacctctggt
    27301 gctgcacatt caaaagagat ttgcagtttt tccagaagag cgcgacgcca tggcgaggtg
    27361 gaggctaaaa taagtttagg catatttttt tccatcagat atagcgtatt gatgatagcc
    27421 attttaaact atgcgcttcg ttttgcaggt tgatgtttgt tatcagcact gaacgaaaat
    27481 aaagcagtaa cccgcaatgt gtgcgaatta ttggcaaaag gcaaccacag gctgcctttt
    27541 tctttgactc tatgacgtta caaagttaat atgcgcgccc tatgcaaaag gtaaaattac
    27601 ccctgactct cgatccggtt cgtacggctc aaaaacgcct tgattaccag ggtatctata
    27661 cccctgatca ggttgagcgc gtcgccgaat ccgtagtcag tgtggacagt gatgtggaat
    27721 gctccatgtc gttcgctatc gataaccaac gtctcgcagt gttaaacggc gatgcgaagg
    27781 tgacggtaac gctcgagtgt cagcgttgcg ggaagccgtt tactcatcag gtctacacaa
    27841 cgtattgttt tagtcctgtg cgttcagacg aacaggctga agcactgccg gaagcgtatg
    27901 aaccgattga ggttaacgaa ttcggtgaaa tcgatctgct tgcaatggtt gaagatgaaa
    27961 tcatcctcgc cttgccggta gttccggtgc atgattctga acactgtgaa gtgtccgaag
    28021 cggacatggt ctttggtgaa ctgcctgaag aagcgcaaaa gccaaaccca tttgccgtat
    28081 tagccagctt aaagcgtaag taattggtgc tccccgttgg atcggggata aaccgtaatt
    28141 gaggagtaag gtccatggcc gtacaacaga ataaaccaac ccgttccaaa cgtggcatgc
    28201 gtcgttccca tgacgcgctg accgcagtca ccagcctgtc tgtagacaaa acttctggtg
    28261 aaaaacacct gcgtcaccac atcactgccg acggttacta ccgcggccgc aaggtcatcg
    28321 ctaagtaatc acgcatctgc gtgatgaagc ttagtgagga ttttccccag gcaactgggg
    28381 aaagaccaaa ccgggcggcg acgatacctt gacacgtcta accctggcgt tagatgtcat
    28441 gggaggggat tttggccctt ccgtgacagt gcctgcagca ttgcaggcac tgaattctaa
    28501 ttcgcaactc actcttcttt tagtcggcaa tcccgacgcc atcacgccat tacttgctaa
    28561 agctgacttt gaacaacgtt cgcgtctgca gattattcct gcgcagtcag ttatcgccag
    28621 tgatgcccgg ccttcgcaag ctatccgcgc cagtcgtggg agttcaatgc gcgtggccct
    28681 ggagctggtg aaagaaggtc gagcgcaagc ctgtgtcagt gccggtaata ccggggcgct
    28741 gatggggctg gcaaaattat tactcaagcc cctggagggg attgagcgtc cggcgctggt
    28801 gacggtatta ccacatcagc aaaagggcaa aacggtggtc cttgacttag gggccaacgt
    28861 cgattgtgac agcacaatgc tggtgcaatt tgccattatg ggctcagttc tggctgaaga
    28921 ggtggtggaa attcccaatc ctcgcgtggc gttgctcaat attggtgaag aagaagtaaa
    28981 gggtctcgac agtattcggg atgcctcagc ggtgcttaaa acaatccctt ctatcaatta
    29041 tatcggctat cttgaagcca atgagttgtt aactggcaag acagatgtgc tggtttgtga
    29101 cggctttaca ggaaatgtca cattaaagac gatggaaggt gttgtcagga tgttcctttc
    29161 tctgctgaaa tctcagggtg aagggaaaaa acggtcgtgg tggctactgt tattaaagcg
    29221 ttggctacaa aagagcctga cgaggcgatt cagtcacctc aaccccgacc agtataacgg
    29281 cgcctgtctg ttaggattgc gcggcacggt gataaaaagt catggtgcag ccaatcagcg
    29341 agcttttgcg gtcgcgattg aacaggcagt gcaggcggtg cagcgacaag ttcctcagcg
    29401 aattgccgct cgcctggaat ctgtataccc agctggtttt gagctgctgg acggtggcaa
    29461 aagcggaact ctgcggtagc aggacgctgc cagcgaactc gcagtttgca agtgacggta
    29521 tataaccgaa aagtgactga gcgtacatgt atacgaagat tattggtact ggcagctatc
    29581 tgcccgaaca agtgcggaca aacgccgatt tggaaaaaat ggtggacacc tctgacgagt
    29641 ggattgtcac tcgtaccggt atccgcgaac gccacattgc cgcgccaaac gaaaccgttt
    29701 caaccatggg ctttgaagcg gcgacacgcg caattgagat ggcgggcatt gagaaagacc
    29761 agattggcct gatcgttgtg gcaacgactt ctgctacgca cgctttcccg agcgcagctt
    29821 gtcagattca aagcatgttg ggcattaaag gttgcccggc atttgacgtt gcagcagcct
    29881 gcgcaggttt cacctatgca ttaagcgtag ccgatcaata cgtgaaatct ggggcggtga
    29941 agtatgctct ggtcgtcggt tccgatgtac tggcgcgcac ctgcgatcca accgatcgtg
    30001 ggactattat tatttttggc gatggcgcgg gcgctgcggt gctggctgcc tctgaagagc
    30061 cgggaatcat ttccacccat ctgcatgccg acggtagtta tggtgaattg ctgacgctgc
    30121 caaacgccga ccgcgtgaat ccagagaatt caattcatct gacgatggcg ggcaacgaag
    30181 tcttcaaggt tgcggtaacg gaactggcgc acatcgttga tgagacgctg gcggcgaata
    30241 atcttgaccg ttctcaactg gactggctgg ttccgcatca ggctaacctg cgtattatca
    30301 gtgcaacggc gaaaaaactc ggtatgtcta tggataatgt cgtggtgacg ctggatcgcc
    30361 acggtaatac ctctgcggcc tctgtcccgt gcgcgctgga tgaagctgta cgcgacgggc
    30421 gcattaagcc ggggcagttg gttctgcttg aagcctttgg cggtggattc acctggggct
    30481 ccgcgctggt tcgtttctag gataaggatt aaaacatgac gcaatttgca tttgtgttcc
    30541 ctggacaggg ttctcaaacc gttggaatgc tggctgatat ggcggcgagc tatccaattg
    30601 tcgaagaaac gtttgctgaa gcttctgcgg cgctgggcta cgacctgtgg gcgctgaccc
    30661 agcaggggcc agctgaagaa ctgaataaaa cctggcaaac tcagcctgcg ctgttgactg
    30721 catctgttgc gctgtatcgc gtatggcagc agcagggcgg taaagcaccg gcaatgatgg
    30781 ccggtcacag cctgggggaa tactccgcgc tggtttgcgc tggtgtgatt gatttcgctg
    30841 atgcggtgcg tctggttgag atgcgcggca agttcatgca agaagccgta ccggaaggca
    30901 cgggcgctat ggcggcaatc atcggtctgg atgatgcgtc tattgcgaaa gcgtgtgaag
    30961 aagctgcaga aggtcaggtc gtttctccgg taaactttaa ctctccggga caggtggtta
    31021 ttgccggtca taaagaagcg gttgagcgtg ctggcgctgc ctgtaaagcg gcgggcgcaa
    31081 aacgcgcgct gccgttacca gtgagcgtac cgtctcactg tgcgctgatg aaaccagcag
    31141 ccgacaaact ggcagtagaa ttagcgaaaa tcacctttaa cgcaccaaca gttcctgttg
    31201 tgaataacgt tgatgtgaaa tgcgaaacca atggtgatgc catccgtgac gcactggtac
    31261 gtcagttgta taacccggtt cagtggacga agtctgttga gtacatggca gcgcaaggcg
    31321 tagaacatct ctatgaagtc ggcccgggca aagtgcttac tggcctgacg aaacgcattg
    31381 tcgacaccct gaccgcctcg gcgctgaacg aaccttcagc gatggcagcg gcgctcgagc
    31441 tttaaaagag gaaaatcatg aattttgaag gaaaaatcgc actggtaacc ggtgcaagcc
    31501 gcggaattgg ccgcgcaatt gctgaaacgc tcgcagcccg tggcgcgaaa gttattggca
    31561 ctgcgaccag tgaaaatggc gctcaggcga tcagtgatta tttaggtgcc aacggcaaag
    31621 gtctgatgtt gaatgtgacc gacccggcat ctatcgaatc tgttctggaa aaaattcgcg
    31681 cagaatttgg tgaagtggat atcctggtca ataatgccgg tatcactcgt gataacctgt
    31741 taatgcgaat gaaagatgaa gagtggaacg atattatcga aaccaacctt tcatctgttt
    31801 tccgtctgtc aaaagcggta atgcgcgcta tgatgaaaaa gcgtcatggt cgtattatca
    31861 ctatcggttc tgtggttggt accatgggaa atggcggtca ggccaactac gctgcggcga
    31921 aagcgggctt gatcggcttc agtaaatcac tggcgcgcga agttgcgtca cgcggtatta
    31981 ctgtaaacgt tgttgctccg ggctttattg aaacggacat gacacgtgcg ctgagcgatg
    32041 accagcgtgc gggtatcctg gcgcaggttc ctgcgggtcg cctcggcggc gcacaggaaa
    32101 tcgccaacgc ggttgcattc ctggcatccg acgaagcagc ttacatcacg ggtgaaactt
    32161 tgcatgtgaa cggcgggatg tacatggtct gaccgcgatt tgcacaaaat gctcatgttg
    32221 cgcgcagtct gcgtggttat gagtaataat tagtgcaaaa tgatttgcgt tattgggggg
    32281 taaggcctca aaataacgta aaatcgtggt aagacctgcc gggatttagt tgcaaatttt
    32341 tcaacatttt atacactacg aaaaccatcg cgaaagcgag ttttgatagg aaatttaaga
    32401 gtatgagcac tatcgaagaa cgcgttaaga aaattatcgg cgaacagctg ggcgttaagc
    32461 aggaagaagt taccaacaat gcttctttcg ttgaagacct gggcgcggat tctcttgaca
    32521 ccgttgagct ggtaatggct ctggaagaag agtttgatac tgagattccg gacgaagaag
    32581 ctgagaaaat caccaccgtt caggctgcca ttgattacat caacggccac caggcgtaag
    32641 tgaacatctc caggcggtcg ttcgaccgcc tgagttttat ctttttgtcc cactagaatc
    32701 attttttccc tccctggagg acaaacgtgt ctaagcgtcg tgtagttgtg accggactgg
    32761 gcatgttgtc tcctgtcggc aataccgtag agtctacctg gaaagctctg cttgccggtc
    32821 agagtggcat cagcctaatc gaccatttcg atactagcgc ctatgcaacg aaatttgctg
    32881 gcttagtaaa ggattttaac tgtgaggaca ttatctcgcg caaagaacag cgcaagatgg
    32941 atgccttcat tcaatatgga attgtcgctg gcgttcaggc catgcaggat tctggccttg
    33001 aaataacgga agagaacgca acccgcattg gtgccgcaat tggctccggg attggcggcc
    33061 tcggactgat cgaagaaaac cacacatctc tgatgaacgg tggtccacgt aagatcagcc
    33121 cattcttcgt tccgtcaacg attgtgaaca tggtggcagg tcatctgact atcatgtatg
    33181 gcctgcgtgg cccgagcatc tctatcgcga ctgcctgtac ttccggcgtg cacaacattg
    33241 gccatgctgc gcgtattatc gcgtatggcg atgctgacgt gatggttgca ggtggcgcag
    33301 agaaagccag tacgccgctg ggcgttggtg gttttggcgc ggcacgtgca ttatctaccc
    33361 gcaatgataa cccgcaagcg gcgagccgcc cgtgggataa agagcgtgat ggtttcgtac
    33421 tgggcgatgg tgccggtatg ctggtacttg aagagtacga acacgcgaaa aaacgcggtg
    33481 cgaaaattta cgctgaactc gtcggctttg gtatgagcag cgatgcttat catatgacgt
    33541 caccgccaga aaatggcgca ggcgcagctc tggcgatggc aaatgctctg cgtgatgcag
    33601 gcattgaagc gagtcagatt ggctacgtta acgcgcacgg tacttctacg ccggctggcg
    33661 ataaagctga agcgcaggcg gtgaaaacca tcttcggtga agctgcaagc cgtgtgttgg
    33721 taagctccac gaaatctatg accggtcacc tgttaggtgc ggcgggtgca gtagaatcta
    33781 tctactccat cctggcgctg cgcgatcagg ctgttccgcc aaccatcaac ctggataacc
    33841 cggatgaagg ttgcgatctg gatttcgtac cgcacgaagc gcgtcaggtt agcggaatgg
    33901 aatacactct gtgtaactcc ttcggcttcg gtggcactaa tggttctttg atctttaaaa
    33961 agatctaagt tgtcattttc cacccttata aaaggtccgc ttgcgggcct tttttcttag
    34021 cttttattcc gacttgttcc gtagtgaaca tgctgccaca ctaacaattc tctgataagg
    34081 agccggtatg ttcttaatta acggtcataa gcaggaatcg ctggcagtaa gcgatcgggc
    34141 aacgcagttt ggtgatggtt gttttaccac cgccagagtt atcgacggta aagtcagttt
    34201 gttatcggcg catatccagc gactacagga tgcttgtcag cggttgatga tttcctgtga
    34261 cttctggcct cagcttgaac aagagatgaa aacgctggca gcagaacagc aaaatggtgt
    34321 gctgaaagtc gtgatcagtc gcggtagtgg cgggcgaggg tacagcacat tgaacagcgg
    34381 accggcaacg cggattctct ccgttacggc ttatcctgca cattacgacc gtttgcgtaa
    34441 cgaggggatt acgttggcgc taagcccggt gcggctgggg cgcaatcctc atcttgcagg
    34501 tattaaacat ctcaatcgtc ttgagcaagt attgattcgc tctcatcttg agcagacaaa
    34561 cgctgatgag gcgctggtcc ttgacagcga agggtgggtt acggaatgct gtgcggctaa
    34621 tttgttctgg cggaagggca acgtagttta tacgccgcga ctggatcagg caggtgttaa
    34681 cggcattatg cgacaattct gtatccgttt gctggcacaa tcctcttatc agcttgtcga
    34741 agtgcaagcc tctctggaag agtcgttgca ggcagatgag atggttattt gtaatgcgtt
    34801 aatgccagtg atgcccgtat gtgcctgtgg cgatgtctcc ttttcgtcag caacgttata
    34861 tgaatattta gccccacttt gtgagcgccc gaattagtca tgaaaaaagt gttattgata
    34921 atcttgttat tgctggtggt actgggtatc gccgctggtg tgggcgtctg gaaggttcgc
    34981 catcttgccg acagcaaatt gcttatcaaa gaagagacga tatttaccct gaagccaggg
    35041 accggacgtc tggcgctcgg tgaacagctt tatgccgata agatcatcaa tcgtccacgg
    35101 gtttttcaat ggctgctgcg tatcgaaccg gatctttctc actttaaagc cgggacttac
    35161 cgctttacac cgcagatgac cgtgcgcgag atgctgaaat tgctggaaag cggtaaagaa
    35221 gcacagttcc ctctgcgact ggtagaaggg atgcgtctga gcgattacct caagcaattg
    35281 cgtgaggccc cgtatatcaa gcatacgctg agcgatgata agtacgccac cgtagcgcag
    35341 gcacttgaac tggaaaaccc ggagtggatt gaaggttggt tctggccaga cacctggatg
    35401 tataccgcca ataccaccga tgtcgcgtta ctcaagcgag cgcacaagaa aatggtgaaa
    35461 gcggtcgata gcgcctggga agggcgtgcg gacggtctgc cttataaaga taaaaaccag
    35521 ttggtgacga tggcatcaat tatcgaaaaa gaaaccgccg ttgccagtga acgcgataag
    35581 gttgcctcag tatttatcaa ccgtttacgc attggtatgc gcctgcagac cgacccgacc
    35641 gtgatttacg ggatgggaga gcgttataat ggcaaacttt ctcgtgcaga cctggaaacg
    35701 ccgacagcgt ataacaccta taccattacc ggtctgccgc caggtgcgat agcgacgccg
    35761 ggggcggatt cgctgaaggc tgctgcgcat ccggcaaaaa cgccgtatct ctattttgtg
    35821 gccgatggta aaggtggtca cacgtttaat accaatcttg ccagtcataa caagtctgtg
    35881 caggattatc tgaaagtgct taaggaaaaa aatgcgcagt aagtatatcg tcattgaggg
    35941 gctggaaggc gcaggcaaaa ctaccgcgcg taatgtggtg gttgagacgc tcgagcaact
    36001 gggtatccgc gacatggttt tcactcggga acctggcggt acgcaacttg ccgaaaagtt
    36061 aagaagcctg gtgctggata tcaaatcggt aggcgatgaa gtcattaccg ataaagccga
    36121 agttctgatg ttttatgccg cgcgcgttca actggtagaa acggtcatca aaccagctct
    36181 ggctaacggc acctgggtga ttggcgatcg ccacgatctc tccactcagg cgtatcaggg
    36241 cggcggacgt ggtattgacc aacatatgct ggcaacactg cgtgatgctg ttctcgggga
    36301 ttttcgcccc gacttaacgc tctatctcga tgttaccccg gaagttggct taaaacgcgc
    36361 gcgtgcgcgc ggcgagctgg atcgtattga gcaagaatct ttcgatttct ttaatcgcac
    36421 ccgcgcccgc tatctggaac tggcagcaca agataaaagc attcatacca ttgatgccac
    36481 ccagccgctg gaggccgtga tggatgcaat ccgcactacc gtgacccact gggtgaagga
    36541 gttggacgca tgagatggta tccatggtta cgacctgatt tcgaaaaact ggtagccagc
    36601 tatcaggccg gaagaggtca ccatgcgcta ctcattcagg cgttaccggg catgggcgat
    36661 gatgctttaa tctacgccct gagccgttat ttactctgcc aacaaccgca gggccacaaa
    36721 agttgcggtc actgtcgtgg atgtcagttg atgcaggctg gcacgcatcc cgattactac
    36781 accctggctc ccgaaaaagg aaaaaatacg ctgggcgttg atgcggtacg tgaggtcacc
    36841 gaaaagctga atgagcacgc acgcttaggt ggtgcgaaag tcgtttgggt aaccgatgct
    36901 gccttactaa ccgacgccgc ggctaacgca ttgctgaaaa cgcttgaaga gccaccagca
    36961 gaaacctggt ttttcctggc tacccgcgag cctgaacgtt tactggcaac attacgtagt
    37021 cgttgtcggt tacattacct tgcgccgccg ccggaacagt acgccgtgac ctggctttca
    37081 cgcgaagtga caatgtcaca ggatgcatta cttgccgcat tgcgcttaag cgccggttcg
    37141 cctggcgcgg cactggcgtt gtttcaggga gataactggc aggctcgtga aacattgtgt
    37201 caggcgttgg catatagcgt gccatcgggc gactggtatt cgctgctagc ggcccttaat
    37261 catgaacaag ctccggcgcg tttacactgg ctggcaacgt tgctgatgga tgcgctaaaa
    37321 cgccatcatg gtgctgcgca ggtgaccaat gttgatgtgc cgggcctggt cgccgaactg
    37381 gcaaaccatc tttctccctc gcgcctgcag gctatactgg gggatgtttg ccacattcgt
    37441 gaacagttaa tgtctgttac aggcatcaac cgcgagcttc tcatcaccga tcttttactg
    37501 cgtattgagc attacctgca accgggcgtt gtgctaccgg ttcctcatct ttaagagaga
    37561 catcatgttt ttagtcgact cacactgcca tctcgatggt ctggattatg aatctttgca
    37621 taaggacgtg gatgacgttc tggcgaaagc cgccgcacgc gatgtgaaat tttgtctggc
    37681 agtcgccaca acattaccgg gttatttaca tatgcgggat ctggtaggcg aacgtgacaa
    37741 cgtcgtattt tcttgtggcg tgcatccact caaccagaat gatccctacg atgtagaaga
    37801 tttacgctgt ctggcggcag aagagggtgt tgtagcgctg ggtgaaaccg ggctggatta
    37861 ttattacacg ccggaaacta aagtacgtca gcaagagtcc ttcatccatc atatccagat
    37921 tggtcgtgaa ctgaacaagc cggttatcgt ccatacccgt gacgcccgtg ccgatacgct
    37981 ggcaattctg cgcgaagaaa aagtgacgga ttgcggtggc gtactacact gttttacaga
    38041 ggacagagaa acggcgggta aattactgga tctcggattt tacatctcct tttccggcat
    38101 tgtgaccttc cgtaatgcgg agcaactgcg cgatgctgcg cgttatgtcc ccctggatcg
    38161 gttactggtg gaaactgact caccttacct tgcgccggta ccgcatcgag gaaaagagaa
    38221 tcaacctgcg atggttcgtg acgttgcaga atacatggct gtgttgaaag gtgttgccgt
    38281 tgaagaactg gcgcaggtaa ccaccgataa cttcgcccgt ctgtttcaca tcgacgcttc
    38341 ccgccttcaa tccatccgtt gaatgagttt ttttaaagct cgtaattaat ggctaaaacg
    38401 agtaaagttc accgccgaaa attgggcggt gaataaccac gtttgaaata ttgtgacata
    38461 tgttttgtca aaatgtgcaa cttctccaat gatctgaagt tgaaacgtga tagccgtcaa
    38521 acaaattggc actgaattat tttactctgt gtaataaata aagggcgctt agatgccctg
    38581 tacacggcga ggctctcccc ccttgccacg cgtgagaacg taaaaaaagc acccatactc
    38641 aggagcactc tcaattatgt ttaagaatgc atttgctaac ctgcaaaagg tcggtaaatc
    38701 gctgatgctg ccggtatccg tactgcctat cgcaggtatt ctgctgggcg tcggttccgc
    38761 gaatttcagc tggctgcccg ccgttgtatc gcatgttatg gcagaagcag gcggttccgt
    38821 ctttgcaaac atgccactga tttttgcgat cggtgtcgcc ctcggcttta ccaataacga
    38881 tggcgtatcc gcgctggccg cagttgttgc ctatggcatc atggttaaaa ccatggccgt
    38941 ggttgcgcca ctggtactgc atttacctgc tgaagaaatc gcctctaaac acctggcgga
    39001 tactggcgta ctcggaggga ttatctccgg tgcgatcgca gcgtacatgt ttaaccgttt
    39061 ctaccgtatt aagctgcctg agtatcttgg cttctttgcc ggtaaacgct ttgtgccgat
    39121 catttctggc ctggctgcca tctttactgg cgttgtgctg tccttcattt ggccgccgat
    39181 tggttctgca atccagacct tctctcagtg ggctgcttac cagaacccgg tagttgcgtt
    39241 tggcatttac ggtttcatcg aacgttgcct ggtaccgttt ggtctgcacc acatctggaa
    39301 cgtacctttc cagatgcaga ttggtgaata caccaacgca gcaggtcagg ttttccacgg
    39361 cgacattccg cgttatatgg cgggtgaccc gactgcgggt aaactgtctg gtggcttcct
    39421 gttcaaaatg tacggtctgc cagctgccgc aattgctatc tggcactctg ctaaaccaga
    39481 aaaccgcgcg aaagtgggcg gtattatgat ctccgcggcg ctgacctcgt tcctgaccgg
    39541 tatcaccgag ccgatcgagt tctccttcat gttcgttgcg ccgatcctgt acatcatcca
    39601 cgcgattctg gcaggcctgg cattcccaat ctgtattctt ctggggatgc gtgacggtac
    39661 gtcgttctcg cacggtctga tcgacttcat cgttctgtct ggtaacagca gcaaactgtg
    39721 gctgttcccg atcgtcggta tcggttatgc gattgtttac tacaccatct tccgcgtgct
    39781 gattaaagca ctggatctga aaacgccggg tcgtgaagac gcgactgaag atgcaaaagc
    39841 gacaggtacc agcgaaatgg caccggctct ggttgctgca tttggtggta aagaaaacat
    39901 tactaacctc gacgcatgta ttacccgtct gcgcgtcagc gttgctgatg tgtctaaagt
    39961 ggatcaggcc ggcctgaaga aactgggcgc agcgggcgta gtggttgctg gttctggtgt
    40021 tcaggcgatt ttcggtacta aatccgataa cctgaaaacc gagatggatg agtacatccg
    40081 taaccactaa tccgtaagac gttggggaga ctaaggcagc cagatggctg ccttttttac
    40141 aggtgttatt cagaattgat acgtgccggt aatgctgaaa ttacgcggtg tgccgtagac
    40201 gatagaacct tccacgttgg tatcgtaggt tttgtcgaac aggttattga cgttcccctg
    40261 taacgagaag tttttcgtca cctggtagcg ggtgaagaga tccaccagcg cgtagctacc
    40321 ttgctcggcg cggaaggtgc catacggcgt cacggtgtcg gtatacacgc gattttgcca
    40381 gttaacacca ccgccgaccg tcaactctgg catgacaggc aaccgatagc tggtgaacat
    40441 tttaaccgtg gtgcgtggca gattaggatt aacggcgttt ccttcgttat cctctgcaat
    40501 atagcgcgtt gcgccaaatg tcagctgcca gttgtcggta attgcgccgt tgagttcaaa
    40561 ttccacccct ttactgactg tcccatccac cgctttatag gcggtttcgc cgttgctgcc
    40621 ggggataggt gtaccggtgg actgagcgac attatcctgc tcaatacgga agatggctaa
    40681 cgtggtggtc agacggctat tcatccagtc cgatttcaga cccagctcgt agttgttacc
    40741 ggtgattgga gccagatatt tgcctgaact gtcacgatca ttttgcggct ggaaaataga
    40801 ggtatagctg gcgtaggtcg accagttgtc attgatgtca aacaccagac cagcgtaagg
    40861 cgtggtgtgg tttttctcca tgctgtaagt cagcgtatca acccgccagt tggtataacg
    40921 tgcgccgagg atcagatgca gcggatcggc aagggtgaca cgagtggcag catataacga
    40981 tttcatatgt gtggtatcgt cctgcgccag gctctgtggt gaccagtcgg tttgtgggaa
    41041 attgccatta aagttgtaga aactgccaat ttcatccggg aagatgttgg cccatgaact
    41101 gaagtaacga ttgttttgtt tgctgtaact gccaccaaac attagattgt gctgacgacc
    41161 aaacaattca taactaccgt cagcgaacaa atccagcgca tcaactttac gtttgccact
    41221 gttccaaccg gtgccgccga cataatcaaa gccaggtcca taattactgt atggcccaac
    41281 cagcatacca tccgctttgt ttacataggc atcgacatac atcattttgc tgtcaaattc
    41341 gacttcagag tgggtggcat tcagtgtcgc ttgccaggta tcagcaaact gctgcttcag
    41401 ggtcataaag accttgttga tctctttatc gttgtacgcc agtcaggtgc ggtactgcgt
    41461 gcgcgatcgt aactgttgct gctgccatca gtattccagc gcggtaaacc gccccaggta
    41521 gggctattaa catcaatgcg ctgatattcg taaccggctg aaagcgtcgt aagatcgcct
    41581 aaatcagcat cgacaatgcc cgagaagaag gtcttttcac tgttgtagcg gtccagccat
    41641 gagtcgttat tctggtagcc gccgacaatt cgcgcgcgga ttttaccgtc ttcggtgagt
    41701 gggctttgta aatccgccac ataccgttct ttgttccagc taccgtattc cgccgagaca
    41761 tcgcctttaa attcacgact ggtcgcgtgt tttcgaacca tattaattgc cgcagatgga
    41821 ttacccgtcc cggtcatgag tcctgtcgcg ccacgcacta cttctacgcg ttcaaagagt
    41881 gccatatcag aaagtgcgtc gcccagattc cagcgcgatt caaaataggt ggggatacca
    41941 tcaaccatat agttatcgat ctggaatccg cgggaataat aaagagcacg atcggaatcc
    42001 gcctgacttt tgctgatccc cagcgtgttt tccatcactt cgcccagcgt ttgtaactgc
    42061 tgatcttcca tccgctgctg gctaacaata gtgaccgact gaggaatatc acgttgagtc
    42121 atctgcattt tggtacccgc agaggtagac gttacgctgt aatcattttc gccatcatct
    42181 ggagctgtgg ctgaaccctc aacaatcacc gtttcttcag tggctggtgc agcaaaagcg
    42241 gcagaaggta atagtgccag tgctatgcaa ccggcaagta gtgacggttt ggtgatggct
    42301 tgatattgat tatccctgtt aaattgtgtt gaaagcattt gaaatctctt ggtttatgtt
    42361 gtcgtcaggc tcatgccgga ggagggtgat gagcgattat atttagtgtg cgaataattt
    42421 tgtttgtaaa tgcaaatgag aaatatacgc attcatattt gtcatgtaaa ccaaacagag
    42481 aatgtctttt cagcgcattc gcaggcagaa atgggaataa tggcgatata tacggcagca
    42541 aaacgatttt ttgcttaaga aatcgtgagt taaggttgaa agagcaggtt taactcgacc
    42601 atactctata ctcgcagtgt ggcgcggcgt agcatggcgc aacgcatggc tatttgaaaa
    42661 aggaaaatgt cgtggcagaa gaaactatat tcagcaaaat tattcgtcgt gagatcccct
    42721 ccgatatcgt ctaccaggat gatctggtaa cggcgtttcg cgatatttcg ccgcaagcgc
    42781 caacgcatat tctgatcatt ccgaatatcc tcattccgac tgtgaacgac gtctcagctg
    42841 agcatgagca ggcgctggga cgcatgatca ccgtagcggc aaaaattgct gagcaagaag
    42901 gtattgccga agatggctat cgtctgatca tgaataccaa ccgccatggc ggacaagagg
    42961 tttaccacat ccatatgcac ttgttgggtg gccgtccgct gggaccaatg ctggcgcata
    43021 aaggtctgta acgatgagaa aaggatgctt tgggctggtg tctctggtgt tgttactgct
    43081 ggtgggctgt cgttcacatc cggaaattcc ggtgaatgat gagcaatcgc tggtgatgga
    43141 gtcatcttta ttggctgcgg gcatcagtgc agaaaagccc ttcctttcga cgtctgatat
    43201 tcaaccttca gcatcctcaa cgctttataa cgaaaggcaa gaacccgtta ccgttcatta
    43261 tcgtttttac tggtatgacg ccagagggct ggagatgcat cctctggaaa ggccacgcag
    43321 cgttaccatt cccgcacatt cggcggtaac gctgtacggc agcgccaatt ttctgggggc
    43381 gcacaaagtc agactttatc tatatttgta aggggtgaat cttgatgaca aaaatgagtc
    43441 gctacgcctt gattaccgcg ctggcgatgt ttctcgccgg gtgtgtgggg caacgtgaac
    43501 ctgcaccggt agaagaagtg aaaccagcgc cggaacaacc agccgagcca caacagcctg
    43561 tccccacagt gccctcggtg ccgacgatcc cgcagcagcc aggcccaatt gagcacgaag
    43621 atcaaactgc accgcctgcg ccgcatattc gccattatga ctggaatggc gcaatgcagc
    43681 cgatggtcag taagatgctt ggggctgacg gggtgactgc gggtagcgtc ctgctggttg
    43741 atagcgttaa caaccgtact aacggttcgc tgaatgccgc agaagcgacc gaaacgctgc
    43801 gaaatgcgct ggctaataac gggaaattta ccctggtttc cgcccagcaa ctgtcgatgg
    43861 cgaagcaaca gttaggtttg tcgccgcagg acagtttagg cacccgtagt aaagccatag
    43921 gcattgcccg caatgtcggc gctcattacg tgctgtactc cagcgcctct ggcaacgtta
    43981 acgctccgac cctacaaatg cagctgatgc tggtgcagac gggcgaaatt atctggtcag
    44041 gtaaaggtgc cgtttcgcag caataatccc atcacgcgcg acgaattgct gtcgcgcttt
    44101 ttcccgcagt atcatcccgt cacgacgttt aatagtgggc ttagtggcgg gagttttctc
    44161 attgaacatc agggccagcg ttttgttgtg cgtcagccgc acgatcctga tgcgccgcag
    44221 tccgcgttct tgcgccagta tcgggcttta tcacaactac ccgcatgcat tgcaccgaag
    44281 ccgcatttat atctccgtga ctggatggta gtcgactatc tgcccggcgc ggtaaaaacg
    44341 tatttgccgg ataccaacga actggcaggc ttgctgtatt atctacatca acaaccacgt
    44401 tttggctggc gaataacgct gttgccgtta ctggaactgt actggcagca aagcgatccg
    44461 gcgcggcgga cagtgggttg gctgcgaatg ttaaaacgtc tgcgcaaagc gcgggaacca
    44521 cggcctttac gcttaagtcc attgcatatg gatgtccacg ccggaaattt agtgcatagc
    44581 gcgtcagggt taaaactcat cgactgggag tatgccggag atggtgatat cgcgctggaa
    44641 ctggcggcgg tgtgggtgga aaatactgaa cagcaccggc aattggtcaa tgactatgcc
    44701 actcgcgcga agatttatcc ggcgcaatta tggcgtcagg tcaggcgatg gtttccctgg
    44761 ctgctgatgc tcaaagcagg gtggtttgag taccgctggc gacaaaccgg cgatcaacaa
    44821 tttatcaggc tggccgatga cacctggcgg cagctattaa taaaacaata aggagagcag
    44881 tgtgggtcca gtaatgttgg atgtcgaagg ttacgaactg gacgcggaag agcgtgaaat
    44941 actggcgcat ccgctggtgg gagggctgat tctctttacg cgtaactatc atgatcctgc
    45001 ccagttacgt gaactggtgc gccagatccg cgcagcttcg cgcaatcgtc tggtggtggc
    45061 ggttgatcag gaaggtggac gcgtgcagcg ttttcgtgaa ggttttaccc gcttgccagc
    45121 ggcgcaatca ttcgctgcgc tgtcaggaat ggaagagggt ggcaaactgg cgcaggaggc
    45181 aggttggttg atggccagcg aaatgatcgc tatggatatt gatatcagct ttgcgcctgt
    45241 gctggatgtc gggcatatca gcgcggcgat tggcgagcgt tcttatcatg ccgatccaca
    45301 aaaagccctg gcaattgcca gccggtttat tgatggtatg catgaagccg gaatgaaaac
    45361 gaccgggaaa cacttcccag gacacggtgc agtaacggca gactcacaca aagaaacacc
    45421 gtgcgatcca cgtccacaag cggagattcg cgctaaagat atgtcggtct tcagttcctt
    45481 aatccgcgaa aataaactcg acgccattat gcctgcgcat gtgatctaca gtgatgttga
    45541 tccgcgtccg gcgagcggtt ctccctactg gctgaaaacc gttttgcgtc aggaactggg
    45601 ttttgacggc gtgattttct ctgacgattt atcgatggaa ggtgccgcga ttatgggcag
    45661 ttatgccgaa cgcgggcagg cttcactgga tgcgggttgc gatatgatcc tggtctgcaa
    45721 taatcgtaaa ggggccgtca gcgtgttaga taatctgtca ccgatcaagg cagaacgtgt
    45781 tacacgtttg tatcataaag gttcattttc gcgacaggaa ctgatggact cggctcgctg
    45841 gaaagcgatc agcacccgtc tgaatcagtt acatgaacgc tggcaggaag agaaagcagg
    45901 tcactaaccc tggcttatgt gaggaagcga tgattatcta tttacacggt tttgactcta
    45961 acagtccggg taaccacgag aaagtcttac aattgcagtt tattgacccg gatgtacgct
    46021 tgataagcta cagtacgcgg catccgaaac atgatatgca gcatctgctt aaagaagtgg
    46081 acaaaatgtt gcaactgaac gttgacgagc gtccgctaat ttgcggcgtt ggcttgggcg
    46141 gatactgggc ggaacggatt ggttttcttt gcgacatccg ccaggtgatc ttcaacccta
    46201 atttgttccc ttatgagaac atggaaggca agattgatcg cccggaagag tatgccgata
    46261 ttgccactaa gtgtgtgacc aacttccgtg agaagaatcg cgatcgttgc ctggtgattt
    46321 tgtcgcgtaa tgatgaagcg cttaacagcc agcggacatc tgaagagttg catcattatt
    46381 acgagattgt ctgggacgaa gagcagacgc acaaattcaa gaatatctcc ccgcatttac
    46441 agcgcattaa agcgttcaaa accctcgggt aaatgccctc gtcgcatcag gtaaccttgc
    46501 cggtacctga tgcgctccga attctgtggg tcggataagg cgtccacgcc gcatccgaca
    46561 gtcgagcatc aatgcctgat gcgcttctta tcaggcctac cgaacgccct gcatacaccc
    46621 ctcactctat atcactctca caaattcgct caaataataa acaataaact ctgttttttg
    46681 atctcacccg gtaaagtcgc ctatcttttc agcaacaaaa cttgattaac atcaattttg
    46741 gtatgaccaa tgcaccattc atgttattct caatagcgaa gaacattttc attgctgtaa
    46801 cctgttgtta attaagagct atgttaataa ccattaatta acaattggtt aataaattta
    46861 agggggtcac gttgactacg ccattgaaaa agattgtgat tgtcggcggc ggtgctggtg
    46921 ggctggaaat ggcaacacag ctggggcata agctgggacg caagaaaaaa gccaaaatta
    46981 cgctggtcga tcgtaaccac agccacctgt ggaaaccgct gctgcacgaa gtggcgactg
    47041 gctcgcttga tgaaggcgtc gatgcgttga gctatctggc ccatgcgcgc aatcatggtt
    47101 tccagttcca gctgggttcc gtcattgata ttgatcgtga agcgaaaaca atcactattg
    47161 cagaactgcg cgacgagaaa ggtgaactgc tggttccgga acgtaaaatc gcctatgaca
    47221 ccctggtaat ggcgctgggt agcacctcta acgatttcaa tacgccaggt gtcaaagaga
    47281 actgcatttt cctcgataac ccgcaccagg cgcgtcgctt ccaccaggag atgctgaatt
    47341 tgttcctgaa atactccgcc aacctgggcg cgaatggcaa agtgaacatt gcgattgtcg
    47401 gcggcggcgc gacgggtgta gaactctccg ctgaattgca caacgcggtc aagcaactgc
    47461 acagctacgg ttacaaaggc ctgaccaacg aagccctgaa cgtaacgctg gtagaagcgg
    47521 gagaacgtat tttgcctgcg ttaccgccac gtatctctgc tgcggcccac aacgagctaa
    47581 cgaaacttgg cgttcgcgtg ctgacgcaaa ccatggtcac cagtgctgat gaaggcggcc
    47641 tgcacactaa agatggcgaa tatattgagg ctgatctgat ggtatgggca gccgggatca
    47701 aagcgccaga cttcctgaaa gatatcggtg gtcttgaaac taaccgtatc aaccagctgg
    47761 tggtggaacc gacgctgcaa accacccgcg atccagacat ttacgctatt ggcgactgcg
    47821 cgtcatgccc gcgtccggaa gggggctttg ttccgccgcg tgctcaggct gcacaccaga
    47881 tggcgacttg cgcaatgaac aacattctgg cgcagatgaa cggtaagccg ctgaaaaatt
    47941 atcagtataa agatcatggt tcgctggtat cgctgtcgaa cttctccacc gtcggtagcc
    48001 tgatgggtaa cctgacgcgc ggctcaatga tgattgaagg acgaattgcg cgctttgtat
    48061 atatctcgct ataccgaatg catcagattg cgctgcatgg ttactttaaa accggattaa
    48121 tgatgctggt ggggagtatt aaccgcgtta tccgtccgcg tttgaagttg cattaatcga
    48181 cgtacactgg cggatgtggc ataaacgcct catccgccct tgaggaacag cgcgatcggc
    48241 agccgcgttg tatcaggcat cctttcagac tcctccgaat ccttaagtat ttccagccat
    48301 tcccgcgctt ttcatcttct gtctgatagc tgcttttctc cttcgcttgc atgattggca
    48361 taactgcaaa gaaggaggtg ttcccgtgaa taaatcaatg ttggcgggta tcgggattgg
    48421 tgtcgcagct gcgctgggcg tagcggcagt ggccagtctg aacgtgtttg aacggggccc
    48481 gcaatacgct caggttgttt ctgcaacccc aatcaaggaa acggttaaaa caccgcgtca
    48541 ggagtgtcgc aacgtcacag tgacccatcg tcgaccggtg caggatgaaa atcgcattac
    48601 ggggtcggtg ctcggcgctg ttgctggcgg cgtgataggg catcagtttg gtggtggtcg
    48661 cggtaaagat gtcgccactg ttgtgggggc gctgggtggt ggatatgccg gtaaccagat
    48721 ccagggctct ctccaggaaa gcgatactta cacgactacg caacagcgtt gtaaaacggt
    48781 gtatgacaag tcagaaaaaa tgctcggtta tgatgtgacc tataagattg gcgatcagca
    48841 gggcaaaatc cgcatggacc gcgatccggg tacgcagatc ccgctagata gcaacgggca
    48901 actgattttg aataacaaag tataacaagg ctgtactctg caatttggcc cctcattcgc
    48961 tcaggctgag gggctttttt tgcgacttat ttcaccagtt cgggccataa acgcaaagtc
    49021 gttccggcaa tctgcatcag tttttccagc gatgcacctt cgcgtgcgct gatcgacatc
    49081 ccctgaataa tacaattaag gaattctgcc agatgtgtca cgtcacagtg gggcgggatt
    49141 tccccgcgcg cttgtcgttg acataaaaac tgctgcaaag tgcgctcttg catcgcatgg
    49201 cgtgatttca acgtattggc gatatctcct gaatctgggg agagggtggt gcagttgttg
    49261 atcatgaagc agcctgccgg ggtgtctttg ctggtaaagc agttggcgat ggcagcaaaa
    49321 tagtcagcca gcgcagactc cacgcttttc tcttcacaaa acagctgtgc ttcatgctta
    49381 gcggcaaaac gatcgatata gcggtcgaga acggcacgaa ataacccctc tttgttggta
    49441 aattccgcgt atagcgtggg cgctttggct ccggtcgctt cgacgaggtc cgcaagagaa
    49501 gtcgcttcat aaccgtgttg ccagaacaat ttcatggcct tatcaagcgc ggcatccctg
    49561 tcgaacactt ttgggcggcc acggcttttt tttacacatt gtgttgagtc agttgccatg
    49621 atgccgttgt acctggtgac tgtgaatgaa aggttattat aaaaataatc acctccgttc
    49681 accagt
//
LOCUS       DBBGLQ010000032        46725 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.032, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000032 DBBGLQ010000000
VERSION     DBBGLQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.032"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            38..5982
                     /locus_tag="ACIYSB_002364"
                     /pseudo
     CDS             38..5982
                     /locus_tag="ACIYSB_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            6190..7050
                     /gene="pdxI"
                     /locus_tag="ACIYSB_002365"
     CDS             6190..7050
                     /gene="pdxI"
                     /locus_tag="ACIYSB_002365"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGC5429405.1"
                     /translation="MSSKTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     AMALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLAAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMIGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            7114..9420
                     /gene="ydbD"
                     /locus_tag="ACIYSB_002366"
     CDS             7114..9420
                     /gene="ydbD"
                     /locus_tag="ACIYSB_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGC5429406.1"
                     /translation="MFKPRICGWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIITDSPLLITGSINASGLVFIDSYYDNPSAIKGSINARGI
                     FINDIIAPVVVSSINSEFMVRAGDKNDTENVKKALTIINPDAYYWGLINDEDALKEIF
                     KRSNIRIAGNVCNQMKKEALFRPKPSPELVQELQMLDEGKVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYIYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSEAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            9591..9785
                     /locus_tag="ACIYSB_002367"
                     /pseudo
     CDS             9591..9785
                     /locus_tag="ACIYSB_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
     gene            9777..11255
                     /locus_tag="ACIYSB_002368"
                     /pseudo
     CDS             9777..11255
                     /locus_tag="ACIYSB_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
     gene            11245..12561
                     /gene="ynbD"
                     /locus_tag="ACIYSB_002369"
     CDS             11245..12561
                     /gene="ynbD"
                     /locus_tag="ACIYSB_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGC5429407.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGDLRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(12612..13217)
                     /gene="azoR"
                     /locus_tag="ACIYSB_002370"
     CDS             complement(12612..13217)
                     /gene="azoR"
                     /locus_tag="ACIYSB_002370"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGC5429408.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            13418..17320
                     /gene="hrpA"
                     /locus_tag="ACIYSB_002371"
     CDS             13418..17320
                     /gene="hrpA"
                     /locus_tag="ACIYSB_002371"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGC5429409.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQVSAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            17592..18392
                     /gene="ydcF"
                     /locus_tag="ACIYSB_002372"
     CDS             17592..18392
                     /gene="ydcF"
                     /locus_tag="ACIYSB_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGC5429410.1"
                     /translation="MNNTPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNVDSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVTHAWQTLKHDAVLIEAMESRSLR"
     gene            18589..20028
                     /gene="aldA"
                     /locus_tag="ACIYSB_002373"
     CDS             18589..20028
                     /gene="aldA"
                     /locus_tag="ACIYSB_002373"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGC5429411.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISSIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(20070..21071)
                     /gene="gap"
                     /locus_tag="ACIYSB_002374"
     CDS             complement(20070..21071)
                     /gene="gap"
                     /locus_tag="ACIYSB_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGC5429412.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTTEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            21260..21790
                     /gene="cybB"
                     /locus_tag="ACIYSB_002375"
     CDS             21260..21790
                     /gene="cybB"
                     /locus_tag="ACIYSB_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGC5429413.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPFASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            22035..22208
                     /locus_tag="ACIYSB_002376"
     CDS             22035..22208
                     /locus_tag="ACIYSB_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429414.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            22234..22302
                     /locus_tag="ACIYSB_002377"
     CDS             22234..22302
                     /locus_tag="ACIYSB_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429415.1"
                     /translation="MNPLVRCTGKIPALALTWTCRP"
     gene            complement(22280..22429)
                     /gene="hokB"
                     /locus_tag="ACIYSB_002378"
     CDS             complement(22280..22429)
                     /gene="hokB"
                     /locus_tag="ACIYSB_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGC5429416.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            22818..24458
                     /gene="trg"
                     /locus_tag="ACIYSB_002379"
     CDS             22818..24458
                     /gene="trg"
                     /locus_tag="ACIYSB_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGC5429417.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(24496..25419)
                     /gene="ydcI"
                     /locus_tag="ACIYSB_002380"
     CDS             complement(24496..25419)
                     /gene="ydcI"
                     /locus_tag="ACIYSB_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGC5429418.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGRSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            25636..26979
                     /gene="ydcJ"
                     /locus_tag="ACIYSB_002381"
     CDS             25636..26979
                     /gene="ydcJ"
                     /locus_tag="ACIYSB_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGC5429419.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLCNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            27204..28859
                     /gene="opgD"
                     /locus_tag="ACIYSB_002382"
                     /gene_synonym="mdoD"
     CDS             27204..28859
                     /gene="opgD"
                     /locus_tag="ACIYSB_002382"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGC5429420.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            28999..29223
                     /gene="ydcH"
                     /locus_tag="ACIYSB_002383"
     CDS             28999..29223
                     /gene="ydcH"
                     /locus_tag="ACIYSB_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGC5429421.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            29286..29825
                     /gene="rimL"
                     /locus_tag="ACIYSB_002384"
     CDS             29286..29825
                     /gene="rimL"
                     /locus_tag="ACIYSB_002384"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HGC5429422.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(29817..30797)
                     /gene="ydcK"
                     /locus_tag="ACIYSB_002385"
     CDS             complement(29817..30797)
                     /gene="ydcK"
                     /locus_tag="ACIYSB_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGC5429423.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQSAHISDSVTIRGSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLVGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            30921..31913
                     /gene="tehA"
                     /locus_tag="ACIYSB_002386"
     CDS             30921..31913
                     /gene="tehA"
                     /locus_tag="ACIYSB_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGC5429424.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLTEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            31910..32503
                     /gene="tehB"
                     /locus_tag="ACIYSB_002387"
     CDS             31910..32503
                     /gene="tehB"
                     /locus_tag="ACIYSB_002387"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGC5429425.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            32806..33474
                     /gene="ydcL"
                     /locus_tag="ACIYSB_002388"
     CDS             32806..33474
                     /gene="ydcL"
                     /locus_tag="ACIYSB_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGC5429426.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILSYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNENTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(33509..34720)
                     /gene="ydcO"
                     /locus_tag="ACIYSB_002389"
     CDS             complement(33509..34720)
                     /gene="ydcO"
                     /locus_tag="ACIYSB_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HGC5429427.1"
                     /translation="MLRYLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTNDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            34773..35309
                     /gene="sutR"
                     /locus_tag="ACIYSB_002390"
     CDS             34773..35309
                     /gene="sutR"
                     /locus_tag="ACIYSB_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGC5429428.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGREQIVHFHSLIHYPRS"
     gene            35382..37343
                     /gene="rlhA"
                     /locus_tag="ACIYSB_002391"
     CDS             35382..37343
                     /gene="rlhA"
                     /locus_tag="ACIYSB_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGC5429429.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMSNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(37435..37665)
                     /gene="yncJ"
                     /locus_tag="ACIYSB_002392"
     CDS             complement(37435..37665)
                     /gene="yncJ"
                     /locus_tag="ACIYSB_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGC5429430.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            37887..38063
                     /gene="hicA"
                     /locus_tag="ACIYSB_002393"
     CDS             37887..38063
                     /gene="hicA"
                     /locus_tag="ACIYSB_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGC5429431.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPCHPSDEIK
                     EPLRKAILKQLGLS"
     gene            38109..38525
                     /gene="hicB"
                     /locus_tag="ACIYSB_002394"
     CDS             38109..38525
                     /gene="hicB"
                     /locus_tag="ACIYSB_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGC5429432.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDVVQLAAKALGKELSLVMV"
     gene            38604..40010
                     /gene="ydcR"
                     /locus_tag="ACIYSB_002395"
     CDS             38604..40010
                     /gene="ydcR"
                     /locus_tag="ACIYSB_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGC5429433.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            40255..41400
                     /gene="ydcS"
                     /locus_tag="ACIYSB_002396"
     CDS             40255..41400
                     /gene="ydcS"
                     /locus_tag="ACIYSB_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGC5429434.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            41418..42431
                     /gene="ydcT"
                     /locus_tag="ACIYSB_002397"
     CDS             41418..42431
                     /gene="ydcT"
                     /locus_tag="ACIYSB_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5429435.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            42432..43373
                     /gene="ydcU"
                     /locus_tag="ACIYSB_002398"
     CDS             42432..43373
                     /gene="ydcU"
                     /locus_tag="ACIYSB_002398"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5429436.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            43363..44157
                     /gene="ydcV"
                     /locus_tag="ACIYSB_002399"
     CDS             43363..44157
                     /gene="ydcV"
                     /locus_tag="ACIYSB_002399"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5429437.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            44179..45603
                     /gene="patD"
                     /locus_tag="ACIYSB_002400"
     CDS             44179..45603
                     /gene="patD"
                     /locus_tag="ACIYSB_002400"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGC5429438.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAVVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(45700..45795)
                     /gene="yncL"
                     /locus_tag="ACIYSB_002401"
     CDS             complement(45700..45795)
                     /gene="yncL"
                     /locus_tag="ACIYSB_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGC5429439.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            45990..46163
                     /gene="ortT"
                     /locus_tag="ACIYSB_002402"
     CDS             45990..46163
                     /gene="ortT"
                     /locus_tag="ACIYSB_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGC5429440.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            46249..46482
                     /gene="ydcY"
                     /locus_tag="ACIYSB_002403"
     CDS             46249..46482
                     /gene="ydcY"
                     /locus_tag="ACIYSB_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGC5429441.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEVRHEQLTKGGTIL"
     gene            complement(46483..>46725)
                     /locus_tag="ACIYSB_002404"
     CDS             complement(46483..>46725)
                     /locus_tag="ACIYSB_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGC5429442.1"
                     /translation="RWWTLIPGLLGSFFVFASISGYQNVGAATTIAVLVASQLIGGLV
                     LDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
BASE COUNT        11843 a        11020 c        12291 g        11571 t
ORIGIN      
        1 ctgggctgcg taatcatcta tttatgagga atttcatatg caaaggaaaa aaatgcttag
       61 tgtctgtgtt gcaatggcgt taagcagcca gacctgggca gcagatacgt cgatagcgga
      121 aacatcggat gagtctcgta aatccagcaa gattagctgc ccggtcaata ttaaatcact
      181 gagtaaagaa cagttagaaa agctgccagc agagtgtaaa tcagctgaag atagtgagtt
      241 gttaccctgg gctgcagtgg gcctggctgc ggtggcaaca ggcgtagcgg tgtatgcact
      301 ccacgatgat aataatcacc atcacgacga tccatctccg gtgccggacg atggcggcaa
      361 cacaccagta ccgccggacg atggcggcga caccccggta ccgccggacg atggcggcga
      421 cacaccagta ccaccggatg atggcggcga cacaccagta ccaccggatg atggcggcga
      481 cacaccagta ccaccggacg atggcggcga caccccggta ccgccggacg atggcggcga
      541 caccccggta ccgccggacg atggcggcga caccccggta ccaccggacg atggcggcga
      601 caccccggta ccgccggatg atggcggcga caccccggta ccgccggatg atggcgacga
      661 cacaccagtc ccaccggacg atggtggcga cacaccagtc ccaccggacg atggcggcga
      721 caccccggtg ccaccggacg atggcggtga caccccggta ccaccggacg atggcggcga
      781 caccccggtc ccaccggacg atggcggcga caccccggta ccaccggacg atggcggtga
      841 caccccggta ccaccggacg atgatggtga cgataccccg gacgacgatg gtgatgacac
      901 cccagtcaaa cacgatcctg tcgtttataa aaataatgtc acctgggatc aggatgcgaa
      961 gaccgtagaa attcgtaata ccaccttcac ctacgctaaa aatgaagacg gcagttacac
     1021 acttaccggg ccaaatggca aaacgacgat tgtaaaaacc tggtccgttg acgaggcggc
     1081 caacactgtc agctttgacg gcgtcaatac ggcgggcggg atcacctggc gttatgatga
     1141 cgatggcaaa atccatattg tcaaagaggc gggtacggtt gctgatggaa ctacaggtga
     1201 ccatttccag gttaacgatg ccaccatcac cgataagggt ggaaataccg cgctgaatgg
     1261 tggcacggtg atggttgttg acggtcacga tataaccctt aataacgacg gtaaaaccac
     1321 cgcaattggc gaaggctcag tggtcggtat cctgaccggc gacgatatca ccatcaataa
     1381 taatggtgaa acggaagttg atggcgggac ggcggttatt atcaatggcg atcgcgcgac
     1441 attgaatacc gcgggtgact cgacgtttac caatggtggg accggcagcg agattaatgg
     1501 cgacaacgcg gtagtaagta ataaaggtgt catgaccgtc gacggagaac attcgacggg
     1561 gtcgaaaatt accggtaacg atgcactggt taaacaatca ggcgatcttt atgtaagcgg
     1621 tggcgcacgt ggaattattg ttgatggaga tggcaccatt atcagcaata aaggcaatat
     1681 aaccgtcgtg gatgagaaat ccattggcat tgagttgcag ggtaatgaca ccacatttat
     1741 caatatgggt gatatttccg ccagcaatgg caatgcggaa gagaatgcca caggtgtgaa
     1801 gattgcgggg gataacgcca cgtttatgaa cgtgggtgat atttctgcca gcaatgcagg
     1861 gacgggagtt caggttattg gcgacgctgg cgatatctca cttgcgggtg gtatgtatgt
     1921 gggtgatttc tccacaggtc tggatgtggt tggcaataat aacactatga cgctggccac
     1981 ctatgagctg aacgtcaccg gacaggatgc gacaggggtc aatgtcgccg ggaacgggaa
     2041 taccatcgat atcgccggca atatactggt cgataaaaac cagttggctg ataatgcggc
     2101 agaatatttt tacgacccat ccgttggcgt taatgtcagc ggtgataata atgatattac
     2161 actggatggt cagttaacga tggtcgtgga tagcgaaacc acgaatcgta cctatgcacg
     2221 ttttgacggt agccaggaaa acatctccgg actggtgatc acgggtgatg gtaatacggt
     2281 agcacttaat ggtggcgtgc aattcagggg agaggcaaat accttaacca acggagagga
     2341 tattgcctcc gcacgtaagg gaagcggctc cactcctctt attaaggttg atggcaactc
     2401 atccgtatac ctgaatggtg actcggtaat aggcggtgat ttcccggtag gttactccag
     2461 tattatccag ttgaatgatg gtgcaatgtt gaagataggc gatgatgcct ctttttcaac
     2521 gaaggatgtc aatatattcg aacactattt tgatgcgact cctgcgataa taaatgcgtc
     2581 ttccggttcc caggtcatca atgaagggga tgtcgatgta gcgaatatta aatttattaa
     2641 tgtcaacgat gaaaacagtt cggcggttaa cagcggcagc atcgcattac ttcagtatga
     2701 ttattcgaca ccgacaaatc cagtcccgga accgggtggg gtagcgctaa tggcgcaagg
     2761 aggcgctacg gttacaaata atggattcat cacagcaaaa gttatggagc agtatagcgt
     2821 ggtgaatatg cgaaactccg tgggtgctac tggcagtgat attccgaata atcaaacgga
     2881 gagtatgatt gctctcgaag cctaccataa cagttatgca ctcaatggtg agagtggcgt
     2941 gattgatatg tatggtcgag ggaacattgg catgacggca atagacaact cgacggctga
     3001 gaatgcaggc aatatctcta tcgatgcgtt atgggttgat gctaatgata caacgcaatt
     3061 acgcagcgat atacatggaa atagagccgg agattatgcc gctggcatgt gggtaggcac
     3121 agatgatttt aacggcccca gagttaacgc aaccgcaatt aataaagaag gcggtgtcat
     3181 tactatctat aatgccggcg cgggaatgtt cgctaatggt catggcaata cggtcattaa
     3241 ccaggggact atcaacctgg aaaaaaatga taactatgac gacacgattg gcgcgaataa
     3301 actcgtcggt atggcggttt acaatggcgg aaccgcaatt aatgatcaga ctggcgttat
     3361 taatattaat gccgaaattg gtcaggcatt ttacaatgac ggttccggcg ttattgttaa
     3421 ctacggcact atctgcacgt tcggtatttg tcaggacagt gcgagctata acccgaccga
     3481 cagtgccgtc agccggggat gggataatgg caacgttatt accgctgaag gcgaaacgct
     3541 ggatttaccc acaagcggag gggtaaccaa ccccctggca gagagcgata tttatatcac
     3601 caacgcagga acggtgacgg gcggagccat tacggttgct cataatggca atctgaccaa
     3661 cgaaatcacg ggtaatatca acaaggtggt tatcgccatt gacggtgaat atatcaacca
     3721 ggggataact caatccgttt ctgttgatgg cggcgtcttt aataatgaag gcacagttac
     3781 aggccaggta gttacgacag ttgcgggttc ggtcatcaac aatgctggca ctattagtag
     3841 tatcgaacag tgggccagcg ctgtttataa caccggaacg gtgagcaact ggaaaggctc
     3901 atcggtgtct gccgtcatga acaaccaggc aggtggaacg gtagataagg tcttttttac
     3961 tgctgcatca acgttcaata acgccggaac ggtcaatcat gtcaccgtgg ataaaaaggg
     4021 tgtattcaac aacctgcaag gcggtgtagc aacaattgat gacggtcaat tgtgggcggg
     4081 ttcatttaat aactggggca cggttaacag tgacgctaat attatggccg ctggcaatcc
     4141 acacaccctt tataacggtg agacgggcgt aatcaacggt caaatcaaga ctgccttgaa
     4201 cgtgaacgct aacacgctgg tggtgaacga cggtactatt aatattgaca acactggtaa
     4261 tgtggcgatg tcggcgcatg gcagcgcgaa aatggtcaac aacaatatca ttaacgtcgg
     4321 cacagaaggc acctctgaga ctggcatggt agggatgcaa ctcgaaagca acgcgaaggc
     4381 ggatgcggtc attgaaaata atggcaccat caatatttac gccagtgatt cttttgcatt
     4441 cagccagtta ggcagcgctg gacatatcgt taacaacggt atcgtgtata tcgacgacag
     4501 cgtgacgggt tccggtatct tcaaacaaag tggtatctcg gcagaaggca acggcgaagg
     4561 cggcgacggc acagaagcgc actatgtgga cttcaccgcg ccggatgagc cgacgattac
     4621 gccttatagc ggcggtagct cgtcatcgtc cgtgagcagt gacgacggca tgaataatct
     4681 caacggttac gtggtgggaa cgagtgccga tggcagcgcc ggtaagctca aggtcaacaa
     4741 tgccagcatg aacggcgtgg ggatcaacac cgggtttacc gccggtaccg cagacaccac
     4801 cgttagtttt gataacgtgg tggagggcag caacctgacc gatgcggatg ccatcacctc
     4861 aacgtccgtg gtatggactg ctaaaggcag caccgatgcc agcggtaacg ttgacgtcac
     4921 catgagcaaa aatgcttata ccgatgtggc gactgatgct tcggtgaatg acatcgcgaa
     4981 agcactggat gcgagttaca ccaacaacga actgtttacc agcctgaacg tcggcacgac
     5041 tgctgaactg aacagcgctc tgaaacaggt cagcggtagc caggcaacca cggtattccg
     5101 cgaagcgcgc gtgttaagca accgctttag tatgctggcg gatgccgcgc cgaaagtggg
     5161 caacggtctg gcgttcaacg ttgtcgcgaa aggcgatccg cgtgccgagt taggtaataa
     5221 taccgaatac gacatgctgg cattgcgtaa aaccatcgac ctgagcgaaa gccagaccat
     5281 gagtctggag tacggtatcg cgcgtctgga tggcgacggt gcgcagaaag cgggtgataa
     5341 tggcgtcacc ggtggctaca gccagttctt tggcctgaaa catcagatgt ccttcgacaa
     5401 cggcatgaac tggaataacg cattgcgtat gacgtttaca accttgacag cagccgctcg
     5461 attgcattta gcaacacgaa caaaacggct gataccgacg tgaagcagca gtacctggag
     5521 ttccgtagcg aaggagcgaa gactttcgaa ccgagcgaag gactgaaggt tacgccatat
     5581 gcgggtgtaa aactgcgtca cacactggaa ggtggctatc aggagcgcaa tgccggagac
     5641 tttaacctga gcatgaacag cggcagcgaa acggcggtgg acagcatcgt cgggctgaag
     5701 ctggattatg caggaaaaga cggctggagt gcgaacgcca cgctggaagg cgggccgaac
     5761 ctgagctacg cgaagagcca gcgcacggca agcctggcag gcgcaggcag ccagcacttt
     5821 aacgtcgatg acgatcagaa gggcggcggc atcaatagcc tggcaagcgt cggcgtgaag
     5881 tacagcagca aagaaagttc gctgaatctg gacgcgtacc actggaaaga ggacggtatc
     5941 agcgataaag gcgtgatgct gaactttaag aaaacgttct aatttttagc atgtgatacc
     6001 caaaccgcaa cgctgataca ggttgcggtt ttttattgcc ggatgcggta cttgacgcgt
     6061 tttgttttgg tgtctttcag ggcaatagag caactcatcc tgtaatcttg tttacacctt
     6121 cgcattattt atctcttttc gtttctatac tgatttttct taatccgttt tattacaggg
     6181 cagggtgcga tgagcagcaa aacatttact ctcggtacaa aatccgttaa ccgtcttggt
     6241 tatggcgcga tgcaactggc aggtcctggg gtttttggcc ccccaagaga tcgccacgtc
     6301 gctataaccg tgctgcgtga ggcgatggca ttgggcgtca atcacattga taccagcgac
     6361 ttttatggtc cgcacgtcac caatcagatt atccgcgaag cgctttatcc ttactctgac
     6421 gacctgacaa ttgtgactaa aattggtgcg cggcgtggtg aggatgcttc ctggttggcc
     6481 gcattttctc cggcagagct gcaaaaagcg gtacatgata atctacgtaa tctcgggctg
     6541 gacgtactgg atgtggttaa cctgcgcgtt atgatcgggg atggtcatgg cccggcggaa
     6601 gggtcgattg aagccagcct gaccgtgctg gcagagatgc aacagcaagg cctggtaaaa
     6661 catattggcc tgagcaacgt cacaccgacg caggttgcag aggcgcgtaa gattgccgaa
     6721 atcgtctgcg tgcagaacga atacaacatt gctcatcgtg cggatgatgc aatgattgat
     6781 gctttggccc gcgatggcat tgcctacgtg ccgttcttcc cgctcggtgg ctttacgccg
     6841 ctgcaatcgt ccacgctttc cgatgttgct gcgagcctgg gtgcaacacc aatgcaggta
     6901 gcgctggcgt ggcttttaca acgttcaccg aatattttgc tgatcccagg gacatcttct
     6961 gttgcgcatt tacgggagaa tatggctgct gaaaaattgc atctttctga ggaagtgttg
     7021 tctacattgg atggtatttc gcgagaataa cgaatataca aaagggaaag aagcatttcc
     7081 cttttatttc ttttttaatg gcatggagtg catatgttta agcccaggat ttgcggctgg
     7141 ataggtttac ttcccctgtt tatgctgagc cttccagttc aggcggaact tcgatgtgtt
     7201 gcaaatgcag ttgatattga accatttttt tctgcggcta ccgctgaaga caaacagcaa
     7261 gttgaacaag ctatcaacag tagcgtgaat cttgtcccct tcggtttatc tgcatcggac
     7321 tggaaagtgc atcgtggcga tttagtggta gaaggtaata tagagagtaa tcaaaaattg
     7381 attgttcttg gcaatttgac agtcaaagga aatatttcca ctttctctct ttctaatcca
     7441 tgggttattc tcggtaacgt gacggcaaca aatataatta cagattcacc actgttaatt
     7501 accggttcga taaatgcaag tggactggta tttatcgact catattacga taatccgtct
     7561 gcgattaagg ggagtattaa tgcgcgtggg atatttatca atgacataat tgcacctgtt
     7621 gttgtgtctt cgataaatag tgaattcatg gtccgtgcgg gagacaaaaa tgacactgaa
     7681 aatgtcaaaa aagcgctgac gataataaat cctgatgcat attattgggg cctaataaat
     7741 gatgaagatg ctctgaaaga aatttttaag cgaagcaata ttcgcatagc agggaatgtc
     7801 tgtaatcaga tgaaaaaaga agcgctgttt cgccctaagc cttctcctga gttagtgcag
     7861 gaattgcaaa tgctggatga aggcaaagtt gctgcatttg aaggacgaga cattgcgaca
     7921 tttgatcttg ccataatgcg aactctccca aggcttaaag gaatttcagc taacctacgc
     7981 aaacaactta ttaatagcaa tgatgaacaa acgattgaaa gtatggcgag atatatgcct
     8041 gacaatgaga ttctggagtt gaccgatcag caattgggtt accagcctgt tgttctgggg
     8101 ttgctcgacc gtgaaccgct ctctgtcgaa ataatgacgc gaatgagtca tctacctgat
     8161 ggtgtcggtc cgttgaatct tgcgctacgt gaaaatctcc ctctggatat cgtcatgact
     8221 ctggcgaaaa gagattggga tatgatcatt caggagcttt ataaagatgc gtggttattg
     8281 cccgaatcca ttattgatgg ttatatccgc agtgatgatt cctctattcg tcaggtcggt
     8341 gctggaggac aactcaccta caatcaggca atgcagttgg cgaacgattc atcaaacgat
     8401 gttgtcacaa gcttagcgtt aaagctggca gagatgaaac accatggtca attgttgcgg
     8461 atgacgccac aagagagtga caaaatagca gtatatatat accaaaaatt cgagaatgac
     8521 gatgatctaa taggtgcgtt atttttagcg ttgccagata acttacagtt taattttgtt
     8581 aaaaggatgg agaaaaaatc ccctgcctac ttttgctgtc gggatatgca ggtaattcac
     8641 tctgacgctg ctttacaacg gttattgaca cgtttcaacg atcctgaggg gtggagtaat
     8701 ctggcgaaaa atcagtatct gagcacgtcg atgaaacaaa aaatttggca acgtgcttta
     8761 tcgcatcgga aaaataatcc gaaagcagat tcagaagcat atgaaaccag cgctgatatg
     8821 attttgtctg agctgattag ccacggcgaa gttgatgacc agatgctatt aaatgccacc
     8881 gccctgatac gtttggaaga ctgggacttt ttagagagtg cattagttag ttgggataat
     8941 ttacccgctg ttgttctcaa ggaattacag caaaacacgc cacgcaatga tatttgggcg
     9001 aagttttttc tgaggcagga aaatagctcc cgtgcgcagg ttgatgaagc attacgtgtt
     9061 tattatgcac tcgaccccga tgcgttagca caactggatg tactggcaaa acaaccggat
     9121 cgtatatggt ggagtacact ggcgaaaagt aatctcacat ttttcaagtt cggcgcactt
     9181 aacaaccgcc acacaccgcc tgcagtactg gcggcagaaa ttgatcccga gtggtggatt
     9241 gtggcgatga ataatccccg ttttccagtt gatgtattaa aggcgaggct gaaacgtgat
     9301 cctttgctgg cgttagagct tgttaaccct gaactggatt tagtccgcca gctggcgctt
     9361 aacggtaaga cgcgcgcaat acgggaacaa gcgatgagaa aacttgatga gttgtattga
     9421 tccacaacgt aatgtttctt aaccatttga ttaattgggg ataatcattc ctggcagtga
     9481 gtccccaata ccttgatata ttctgaattt ttaatgaagc ggcgtgttgc gatatctccg
     9541 tcaggggaat tgatgcacca tagcgcaaac cgaattatca aggattgata atgatgctct
     9601 accagataaa accgctcttt cagtcgctgt taaggccgac gatgttttgg ctttataatc
     9661 accacgttac agcgaatcac atcactcttg ctgcactggc gctttctctt cttaccggat
     9721 tgctgttgat gttggtgtca caacccatcc tctttatgct attgcccatc gtgattttta
     9781 tccgttttgc tgccagcgac agccatgtcg gactggaaga ggtggttgtg atcgcgcaaa
     9841 gcgtcggcgc agtgctggtt gcaacttggg ttcatgatta tgcgcctgca attcgcgggc
     9901 tggtgctggc ttctccggcc tttaaggtta aattgtatgt gccgctggca cgtcctgcgc
     9961 tggcgttatg gcatcgtctg cgtggtctgt tttttattaa ttcctatgtg aaaggacgct
    10021 atttgaccca cgatcggcaa cgggtggcga gtttcaataa tgatccgctg atcacacggg
    10081 cgattgccgt caatatcttg ctcgatctct ataaaacgtc tgaacgtatt gttagcgatg
    10141 cggcggcgat tacgctcccc acgcaacttc tgatatcagg cgatgactat gtggtgcatc
    10201 gtcaaccgca gattgatttt tatcagagat tacgtagccc tctgaaagag ctgcatctgc
    10261 tgccaggctt ttatcacgac acgttgggtg aagagaacag ggcgcaggca tttgaaaaaa
    10321 tgcaaagctt tattagtcgt ttatatgcta acaagtcgca aaaatttgat tatcagcatg
    10381 aagaccgcac aggaccatca gcggatcgat ggcggctcct ttctggtgga cccgtgccat
    10441 tatcgccggt tgatttggcg tatcgcttta tgcgaaaggc gatgaaattg ttcggggcgc
    10501 actctgcggg cctgcatctc ggaatgagca ctggctttga ttcaggcagt tcgctggatt
    10561 atgtctatca aaatcaaccg caaggcagta acgcattcgg gcgcttaatc gacaaaatct
    10621 acctgaacag tgttggctgg cgcggtattc gccagcgcaa aacccattta caaatactga
    10681 ttaaacaagc cgttgccgat ctccacgcca aaggtttagc cgtccgcgtg gttgacattg
    10741 ccgcagggca tgggcgctat gtactggatg cactggcaaa cgagcctgcc gtaagcgata
    10801 ttttgttacg tgattacagc gagttaaatg ttgcacaggg gcaagagatg attgctcaac
    10861 ggggaatgtc tgggcgggtg cgttttgaac agggcgatgc gtttaacccg gaggaactca
    10921 gcgcgttaac tccgcggcct acgctggcga ttgtctctgg cctgtatgag ctttttcccg
    10981 aaaatgagca ggtaaaaaac tcactcgcag gtcttgccaa tgccatcgaa ccgggcggca
    11041 ttctcatcta caccgggcag cggtggcacc cacaactgga gctgattgcc ggggtgttaa
    11101 ccagtcataa agatggtaaa ccgtgggtca tgcgcgtgcg ttcgcaagag gagatggatt
    11161 cactcgtgcg tgatgccgga tttgataaat gcacacaacg gattgatgag tggggtattt
    11221 ttacggtttc gatggcggtg cgtcgtgata actgaacgtc ggaacgtatt gctacaaggc
    11281 gctggctggt tattgttgct ggccccgttt ttcttcttca cctatggatc tcttaatcag
    11341 ttcaccgcgg ttcaggacct taacagccat gatatcccca gtcaggtatt cggttgggaa
    11401 acggcgatcc cttttcttcc ctggactatt gttccttact ggagtctgga tcttttatat
    11461 ggattttcgc tgttcgtttg tagcacgaca ttcgaacagc gccgacttgt ccaccggctt
    11521 attctggcaa cggtaatggc ctgctgcggt tttttgctct atccgctgaa gtttagtttt
    11581 atccgtcctg aagtgagtgg ggtgacggga tggctatttt cgcaacttga actgtttgat
    11641 ctgccttata accagtctcc ttcgctgcat attattctct gctggctact ttggcgtcac
    11701 tttcgtcagc atctggctga gaggtggcgt aaagtctgtg gcggatggtt tttactcatc
    11761 gctatttcta ctctgacgac ctggcagcat cattttattg atgtcatcac agggctggcg
    11821 gtaggtatgt tgattgactg gatggtgccc gtcgatcgtc gttggaatta tcagaaacct
    11881 gatcaacgtc gaatcaaaat agcactgcca tatgtcgtag gcgcgggctc gtgcattgtg
    11941 ttgatggagc taatggtgat gattcagtta tggtggtcag tctggttatg ttggccagta
    12001 ttatcgctac tcattattgg ccgtgggtac ggtgggcttg gcgcgataac aacagggaaa
    12061 gatagtcagg ggaaactccc gcccgccgtt tactggctga cattgccctg gcgcatcggg
    12121 atgtggctgt ctatgcgttg gttttgtcgt cgcctggagc cggtgagcaa aattactgct
    12181 ggtgtttatt taggggcgtt tccacgacat attccggcac agaatgcggt tctggacgtc
    12241 acctttgaat tccctcgggg acgagcgaca aaagatcgac tctatttctg tgtaccgatg
    12301 ctggatctgg tggttccgga agagggggat ctccgacagg ccgtggcgat gctggaaaca
    12361 ttacgcgaag agcaaggcag cgttctggtc cattgcgcgt tgggattatc gcgcagtgcg
    12421 ctggtagtgg cggcatggtt gttatgttac ggacactgta aaaccgttga tgaagcgatt
    12481 agttatattc gagccagacg ttcgcatatt gtgcttaagg aagatcacaa agcgatgctg
    12541 aaattatggg aaaacaggta agtggattga gatgtggact gaactacagt ccacatcaag
    12601 atcgtgtccg gttatgcagc aacaatgctg tcgatggccg ctttcgcgtc agactgcgct
    12661 ttcgctgcca tttccggacc gtatgcgatc ccttcggcga agacaaattt cacatcggta
    12721 atgccgataa agccgaggaa cgtggacaga tacggcgtca ccaggtccgt tggtccatct
    12781 ttgtggatcc caccgcggct ggtaataacg atggcttttt tacccgttac cagaccttcc
    12841 ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg caaccaggtc aaaatagttt
    12901 ttcaactgag ttgagatgtt gaagttatac atcggtgccg caataacgat aacgtcgtgg
    12961 gctttcagct cggcaatcag ctcatcggaa agcgccagag cttcctgctg acgcggagtc
    13021 agcggcgcat cgctcggacg cagagcgcca accagttcgc catccagtac cggaatcgga
    13081 tttgcagcca ggtcgcgaac ggtgatttca tcagcggagt gcttttcgcg ccattgttca
    13141 acaaaataat cggacaactg attagactga gagtaccctg ccaggatgct ggatttaaga
    13201 actaatacct tgctcatggt gtttccttat agatgtttga atggacgatg ccccgttgct
    13261 tgctgacact ttattcacaa tcctgccaca gagatagcgc aataaatcga agcctatgtt
    13321 cgaatttatt gaacaacgca tagaaagccg cgatgtggta ctctatatct atcatttaaa
    13381 agaaaattaa tcaggcagac tactgcccac taacgttatg acagaacaac aaaaattgac
    13441 ctttactgct ttgcagcagc ggctggattc gctgatgctg cgcgacagac tgcgtttttc
    13501 tcgccgtctg cacggtgtta agaaggttaa aaatcctgat gcacaacagg ccattttcca
    13561 ggagatggcg aaagagattg accaggcggc agggaaagtc ctgctgcgtg aagcggcacg
    13621 accggaaatc acttatcctg acaatttacc ggttagtcag aaaaaacagg acattctcga
    13681 agcgattcgt gatcaccagg tggtgatcgt cgccggggaa accggttcag gcaaaaccac
    13741 ccagttgccg aaaatctgta tggagctggg gcgcgggatt aaaggactga tcggccatac
    13801 ccagccgcgt cgcctggcgg cacgaacggt ggcgaaccgt attgcggaag agctgaaaac
    13861 ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc gatcacgtta gtgataacac
    13921 gatggtcaag ctgatgaccg acggtatcct gctggcggag atccagcaag accgcctgct
    13981 gatgcagtac gacaccatca ttatcgatga agcacacgaa cgcagcctga atatcgattt
    14041 tctgctcggc tatttgaaag agttgctgcc gcggcgtcct gacctaaaaa tcattatcac
    14101 ttccgcgact atcgacccgg aacgcttttc gcgccacttt aataatgcgc cgattatcga
    14161 agtctccggt cggacctatc cggtggaagt gcgctatcga ccgattgttg aagaagccga
    14221 cgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc gtagacgaac tgagtcagga
    14281 aagccctggc gacattctga tctttatgag cggcgagcgg gaaatccgcg ataccgccga
    14341 tgcgctgaac aagctgaact tacgccatac cgaaatcttg ccgctttatg cgcggctttc
    14401 gaatagcgaa cagaaccgcg tgttccagtc gcacagcggg cgacgcattg tgctggcgac
    14461 caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa tacgttatcg accccggtac
    14521 ggcgcgtatc agccgctaca gctatcgcac caaagtgcag cgtttgccga ttgagccgat
    14581 ttcccaggcg tctgccaatc agcgtaaagg ccgctgtggt cgtgtgtccg aagggatctg
    14641 tattcgtctc tattccgaag acgatttcct ctcgcgcccg gagtttaccg atccggagat
    14701 tctgcgtacc aacctggcat cggttatttt gcagatgacc gcgctggggc tgggcgatat
    14761 cgcggcgttc ccgtttgtcg aagcaccgga taaacgcaat atccaggatg gcgtgcgtct
    14821 gctcgaagaa ctgggcgcga tcaccactga tgaacaggtc agcgcctata aactgacgcc
    14881 gctcggtcgc cagctctcgc agttgcctgt cgacccacgt ttggcgcgta tggtgctgga
    14941 agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc acgtccgcgc tctccattca
    15001 ggatccgcgc gagcgtccga tggataaaca gcaggcatcg gacgaaaaac atcgtcgctt
    15061 ccacgacaaa gaatccgact tcctcgcgtt tgtgaatctg tggaattatc tcggcgagca
    15121 gcaaaaggcg ctctcttcaa acgctttccg tcgcctgtgc cgcaccgatt atctcaacta
    15181 tctgcgcgtg cgcgaatggc aggatatcta cacccagttg cgtcaggtgg tgaaagaact
    15241 tggcattccg gttaacagcg aaccggcgga gtatcgcgaa attcacattg cgttgctgac
    15301 gggtttgctt tcccatatcg gcatgaaaga tgccgataaa caagaatata ccggcgcacg
    15361 taacgcgcgt ttctccatct tccccggttc tggtttattc aaaaaaccgc cgaaatgggt
    15421 aatggtggcg gaactggtag aaaccagccg cctgtggggg cgcattgctg cgcgcatcga
    15481 cccggaatgg gtggagccag ttgctcagca tttgattaaa cgcacctaca gcgaaccgca
    15541 ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa gtcactgttt atggcttgcc
    15601 gattgttgcc gcgcgcaagg tcaactacag ccagattgat ccggcgttat gtcgtgaact
    15661 ctttattcgc cacgcgctgg tggaaggtga ctggcagact cgtcacgcat tcttccgtga
    15721 aaacctgaaa ctgcgggccg aagtggaaga gctggaacac aaatcacgtc gccgcgatat
    15781 tctggttgat gacgaaacgt tgtttgagtt ctacgaccag cgcatcagcc atgatgtgat
    15841 ctctgcacgc cacttcgaca gctggtggaa aaaagtcagc cgcgaaacgc ctgatttgct
    15901 caactttgaa aagagcatgt tgatcaaaga aggggcggaa aaaatcagca agctggatta
    15961 cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg agctatcagt ttgagcccgg
    16021 cgcggatgct gacggtgtga ccgtacatat tccgctgccg ttacttaacc aggttgagga
    16081 gagcgggttt gaatggcaga tccccggtct gcgccgcgaa ctggtgattg ctctgattaa
    16141 atcgttgccg aaaccggtac gccgtaattt tgtacctgcg ccaaactatg ccgaagcgtt
    16201 tttaggccgc gtcacaccgc tggagttacc gttgcttgac agccttgagc gcgagttacg
    16261 gcggatgacc ggcgttaccg ttgaccgcga agactggcac tgggatcagg tgcccgatca
    16321 cctaaaaatc accttccgcg tggtggacga caaaaataag aagctaaaag aagggcgctc
    16381 attacaggat ctgaaagacg ccctgaaagg caaagtgcag gaaacgctgt ctgcggtggc
    16441 ggatgacggt atcgagcaga gcgggctgca tatctggagc tttgggcaac tgccggaaag
    16501 ctacgaacag aagcgtggca actacaaagt gaaggcgtgg ccagcgctag tggatgaacg
    16561 cgacagtgtg gcgatcaaac tgtttgataa cccgctggag caaaagcagg caatgtggaa
    16621 cggtcttcgc cgtctactgc tgctgaatat tccctcgcca atcaaatatt tgcatgagaa
    16681 gttgccaaac aaagccaagc tggggctgta ctttaacccg tatggcaaag tgctggagct
    16741 gatcgacgac tgtatctcct gcggtgtgga taaattgatc gacgccaatg gtggcccggt
    16801 ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg cgcgccgaac tgaatgacac
    16861 ggtggtggat attgcgaagc aggtcgagca aatcctcact gcggtgttca atatcaacaa
    16921 acgtctgaaa gggcgggtgg atatgaccat ggcactgggg ctttctgaca ttaaagcgca
    16981 gatgggcgga ttggtatatc gcggttttgt cactggtaac ggcttcaaac ggctgggcga
    17041 cacgctgcga tatttgcagg cgattgaaaa acgactggaa aaactggcgg ttgatccaca
    17101 tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag caggcgtggc agcaatggat
    17161 caacaaactg ccgcccgcac gtcgtgagga tgaagacgtg aaagagatcc gttggatgat
    17221 agaagagctg cgcgttagtt acttcgctca acaacttggt acgccttatc cgatttcaga
    17281 taagcgtatt ttgcaggcga tggaacagat tagcggttaa cactgctatt tgcctgataa
    17341 atctaaaacc cggtaagcat ttagcgccgg gttttttatt aattctaaaa cggtaagggc
    17401 aaaaattcag gaattcagaa aaatacaatt ctctgctgca agatgaataa tgtttatcta
    17461 cagcatttcc ttaaaagata tgtcaggctt gcggagtggc ggttaaggaa atacgacttc
    17521 ctcctttcag agtgctccgc ttctcactat tatctcacgc agtattctta agggaacgat
    17581 aaggaggaac catgaacaat accccgtttc cgacgctttc gacggcaacc atagatgcca
    17641 taaatgttat tggacagtgg ctggcgcagg atgatttctc cggtgaggtg ccgtatcagg
    17701 ccgattgcgt gatccttgca ggcaatgcgg ttatgccgac tatcgatgcg gcatgtaaga
    17761 ttgcccgcga tcagcaaatt cctttactga ttagtggtgg tatcggtcac tcgacaactt
    17821 ttttgtatag cgccatcgca cagcatccgc actacaacac tatccgcacc actggcagag
    17881 cagaagcgac catcctggcg gatatcgctc atcagttctg gcatattccg catgaaaaaa
    17941 tctggattga agaccagtca acaaactgcg gtgaaaacgc acgctttagc atcgcgctat
    18001 tgaatcaggc cgtagaacga gttcatacgg ctatcgttgt tcaggacccc accatgcagc
    18061 ggcgcacgat ggcgacgttc cgccgtatga ccggggacaa tcccgatgca ccacgctggt
    18121 taagttatcc cggattcgtt cctcagttag gaaataacgt agacagtgta atctttatta
    18181 atcagttaca aggattatgg ccggttgagc gttatctatc actactcact ggcgagctgc
    18241 cgcgtttacg cgatgatagc gatggctacg gtccccgcgg gcgagatttt atcgttcacg
    18301 ttgattttcc ggcagaagtc acccatgcat ggcaaacgct gaaacacgat gcggtgctca
    18361 tcgaggcgat ggaaagtcgc tcgttacgtt aaaaattgcc cgtttgtgaa ccacttgttt
    18421 gcaaacgggc ataactcctg ccttttattt ctgcctctta ttccttttac acttgttttt
    18481 atgaagccct tcacagaatt gtcctttcac gatgccgtct ctctgatgat tgatgttaat
    18541 taacaatgca ttcaccgaaa acaaacatat aaatcacagg agtcgcccat gtcagtaccc
    18601 gttcaacatc ctatgtatat tgatggacag tttgttacct ggcgtggaga cgcatggatt
    18661 gatgtggtaa accctgctac agaggctgtc atttccagca ttcccgatgg tcaggccgag
    18721 gatgcccgta aggcaatcga tgcagcagaa cgtgcacaac cagaatggga agcgttgcct
    18781 gccattgaac gcgccagttg gttgcgcaaa atctccgccg ggatccgcga acgcgccagt
    18841 gaaatcagtg cgctgattgt tgaagaaggg ggcaagatcc agcagctggc tgaagtcgaa
    18901 gtggctttta ctgcggacta tatcgattac atggcggagt gggcacggcg ttacgagggc
    18961 gagattattc aaagcgatcg tccaggagaa aatattcttc tgtttaaacg tgcacttggc
    19021 gtgactaccg gcattctgcc gtggaacttc ccgttcttcc tcattgcccg caaaatggct
    19081 cccgctcttt tgaccggtaa taccatcgtc attaaaccca gtgaatttac gccaaacaat
    19141 gcgattgcat tcgccaaaat cgtcgatgaa ataggccttc cgcgcggcgt gtttaacctt
    19201 gttctggggc gtggtgaaac cgttgggcaa gaactggcgg gtaacccaaa ggtcgcaatg
    19261 gtcagtatga caggcagcgt ctctgcaggt gagaagatca tggcgactgc ggcgaaaaac
    19321 atcactaaag tgtgtctgga attgggtggt aaagcaccag ctatcgtaat ggacgatgcc
    19381 gatcttgaac tggcagtcaa agccatcgtt gattcacgcg tcattaatag tgggcaagtg
    19441 tgtaactgtg cagaacgtgt ttatgtacag aaaggcattt atgatcagtt cgtcaatcgg
    19501 ctgggtgaag cgatgcaggc ggttcaattt ggtaaccccg ctgaacgcaa cgacattgcg
    19561 atggggccgt tgattaacgc cgcggcgctg gaaagggtcg agcaaaaagt ggcgcgcgca
    19621 gtagaagaag gggcgagagt ggcgttgggt ggcaaagcgg tagaggggaa aggatattat
    19681 tatccgccga cattgctgct ggatgttcgc caggaaatgt cgattatgca tgaggaaacc
    19741 tttggcccgg tgctgccagt tgtcgcattt gacacgctgg aagatgctat ctcaatggct
    19801 aatgacagtg attacggcct gacctcatca atctataccc aaaatctgaa cgtcgcgatg
    19861 aaagccatta aagggctgaa gtttggtgaa acttacatca accgtgaaaa cttcgaagct
    19921 atgcaaggct tccacgccgg atggcgtaaa tccggtattg gcggcgcaga tggtaaacat
    19981 ggcctgcatg aatatctgca gacccaggtg gtttatttac agtcttaatg agtgaaagag
    20041 gcggaggttt tttcctccgc ctgtgcgcgt cagagtttag cgaatttttc gagggtgcga
    20101 ataagctgcg tgacgaagcc atattcgtta tcgtaccagg cgaccgtttt caccagttgt
    20161 aaatcgccca cggcggtaat ttccgtttgc gtggcatcaa acaccgaacc gaaatggctg
    20221 ccaatgatat cggaagagac tatttcttca tcggtataac caaatgactc gttattggtg
    20281 gttgcttgtt taagtgcgtt attcacctct tcggtagtca ctttttttcc gagaatggac
    20341 accagctcag tgaccgaacc tgttttcacc ggtacgcgtt gcgcatgacc tttcagtttg
    20401 ccgctcagtt ccggtatcac cagaccaatg gcttttgccg cccccgtagt gtggggaatg
    20461 atattttctg ccgcggcgcg tgaagcacgt aaatctttac cacgcgggcc atccaccagt
    20521 gactgggtgc cagtataggc atgaatggtc gtcatcgtgc cgacttctat cccgaaactg
    20581 tcatgcaagg ctttggccat cggcgcaaga cagttagtgg tgcatgacgc cacggaaaca
    20641 atggtgtcgt tgccatccag agtgtcgtca ttgacgttat aaacgatagt tttcatttca
    20701 ccggcagggg cggaaatcaa caccttcttc gcaccagcat caagatgcgc ctgcgatttc
    20761 tcggcggagg tataaaagcc agtacattcg acaatgattt ctgcaccttt cgctttccac
    20821 ggaatatttt tagcctcttt ttcggcgtaa accgcgatac ttttcccatc aacgataagt
    20881 gaatcttccg taaaatcaac gctccagggg aatggtccgt agtttgaatc atgtttcagc
    20941 aggtaggcga gaatttttgg ggaagtgaga tcattaatag cgacaacgtc tatgttgctt
    21001 ttgacttcaa gtaatcgacg caacaccagt cgaccgatac gaccaaaacc gttaatacca
    21061 actttactca tggttttctc ctgtcaggaa cgttcggatg aaaattgatc ctttccaggc
    21121 ttagaccagg atggcgggat gggcaatctc cattctcaca gtgaaacgta acgtactgaa
    21181 aacgggtgaa caatatttaa tgaaattttg agaaaagccc gttatgttaa cggaaaatta
    21241 tgttaaagca ggaaatgtta tggaaaataa atattcaagg ttacaaatca gcattcactg
    21301 gctggtcttt ttactggtta tcgcagcgta ttgcgcaatg gagtttcggg gtttcttccc
    21361 acgtagcgat cggccgctca tcaacatgat tcatgtttcc tgcggcatct caattctcgt
    21421 gctgatggtg gttcgtcttt tgttaaggct gaaatacccg accccgccga ttatacctaa
    21481 gccaaaaccg atgatgacgg gactggcgca tttgggacat ttggtgattt atctcctgtt
    21541 tattgcgctg ccagtgattg gtttggtgat gatgtataac cggggcaacc cgtggtttgc
    21601 gtttggtttg acgatgcctt tcgcttcaga ggccaatttc gaacgggtag atagcttaaa
    21661 gtcgtggcat gagacgctgg cgaatctggg atattttgtc atcgggttgc acgctgcggc
    21721 tgcactggca caccactatt tctggaagga caacacactt ctacgcatga tgccgcgtaa
    21781 acgttcctga aggatattta aagaaaacgc ctgtactaaa accgacccgt ggtacaggcg
    21841 aagaatacgg gtctacatcg gaagcgccta ttatatttat ttgtatgaca aataaaacca
    21901 taatccttgc ccatacgtcc atctgcctta tttttaatca attcacccga tctttgatct
    21961 catcaacgat atcaaaataa accagcgtat aacgttcaaa tcgaacataa gattcgataa
    22021 accatggagg ttatatgaaa aaactggcac ttattttgtt tatgggaacg cttgtttcct
    22081 tttatgccga tgccgggcgc aaaccctgtt ctggttcgaa aggggggatc tcacactgta
    22141 cggcaggcgg caaatttgtc tgtaatgatg gttctattag tgcatcgaaa aaaacatgca
    22201 ctaactgaag tgtaaaaggg gtgccatgag aaaatgaatc cgctagtcag atgcacgggg
    22261 aaaatccccg cgcttgccct tacctggacg tgcaggccat gagcgcagca acctccttat
    22321 caccgtcccg gaaccgcagt tcgtagagcg tttgtcgggt caggagtgtg aatgtcagaa
    22381 tcgtaatgca gataatgagc agacacacca ccagagggtt gtgcttcata gcctttctcc
    22441 ttgccggatg ccgggtaaga ggctaagatc tgaattgcta ggttcattcg ttggcctcgg
    22501 ttgatagaaa tatcggtctg ggccttcgtc tttctgattc ccggttagcc tgaaaacaga
    22561 aagtctcagg cacccgcagg catcctatga ggtttcctta gggacgaaat aatcacttca
    22621 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg tttagccatt cgttactctc
    22681 ttcattccaa tagcattaat cttctctgca ataattgttg taaaaatgtg acgcaaagag
    22741 gcttttagtc gtaagtaatt accgtcaaat gccgatgact ttctatcagg agtaaacctg
    22801 gacgagagac aacggtaatg aatacaactc cctcacagcg attaggtttt ttgcatcaca
    22861 tcagattggt tccgttattt gcctgcattc taggcggtat cttagttcta ttcgcattaa
    22921 gttcagccct ggctggctat ttcctctggc aggccgatcg cgatcagcgt gatgttactg
    22981 cggagattga gattcggacc gggttagcga acagttcaga ttttttgcgt tcagcccgga
    23041 tcaatatgat tcaggccggg gctgcgagtc gtattgcgga aatggaagca atgaagcgaa
    23101 atattgcgca agccgaatcg gagattaaac agtcgcagca aggttatcgt gcttatcaga
    23161 atcggccggt gaaaacacct gctgatgaag ccctcgacac tgaattaaat caacgcttcc
    23221 aggcttatat cacgggtatg caacctatgt tgaaatatgc caaaaatggc atgtttgaag
    23281 cgattatcaa tcatgaaagt gagcagatcc gaccgctgga taatgcttat accgatattt
    23341 tgaacaaagc cgttaagata cgtagcacca gagccaacca actggcggaa ctggcccatc
    23401 agcgcacccg cctgggtggg atgttcatga ttggcgcgtt tgtgcttgcc ctggtcatga
    23461 cgctgataac atttatggtg ctacgtcgga tcgtcattcg tccactgcaa catgccgcac
    23521 aacggattga aaaaatcgcc agtggcgatc tgacgatgaa tgatgaaccg gcgggtcgta
    23581 atgaaatcgg tcgcttaagt cgtcatttac agcaaatgca gcattcactg gggatgacag
    23641 tagggactgt tcgacagggc gcggaagaga tttatcgtgg caccagcgaa atttcagctg
    23701 gcaatgcgga cctgtcatct cgcaccgaag aacaagcggc ggctatcgaa caaactgccg
    23761 ccagcatgga gcaactcact gcgacggtga aacagaatgc ggataacgcg catcatgcca
    23821 gcaaactggc gcaagaggct tctattaaag ccagcgatgg cgggcagacg gtttccggtg
    23881 tagtaaaaac gatgggcgct atctccacga gttcgaagaa aatttctgag atcaccgccg
    23941 tcatcaacag tattgctttc cagacgaata ttctggcact gaatgctgcc gttgaagccg
    24001 cgcgagcggg tgagcaaggg cgtggatttg ccgttgtcgc cagcgaagta cggacactcg
    24061 caagtcgcag cgctcaggcg gcgaaagaga ttgaaggctt gatcagtgaa tcagtcaggt
    24121 taattgacct ggggtcggat gaggtggcaa cggccgggaa aaccatgagc actattgttg
    24181 atgccgtcgc gagtgtcaca catatcatgc aggaaatcgc cgccgcctcg gatgaacaaa
    24241 gtagaggcat aacgcaggtt agccaggcga tttctgaaat ggataaggtg acgcaacaga
    24301 atgcttctct ggtagaagag gcctcagcgg cggcggtgtc ccttgaagaa caggcggcac
    24361 gattaactga ggcggtggat gtattccgtc tgcacaaaca ttctgtgtcg gcagaacctc
    24421 gcggagcggg tgaaccagtt agtttcgcta cggtgtgaaa atattcaagg agggatcgac
    24481 agatcccttc acctttcaga acggcattga ttttcgaata gcgttaatca tcaactggca
    24541 accagaagag aacgtcgcat ctacgcgggt cagtattcca atcggttcgc ctgcaccatg
    24601 tcccggaaca ggcagggcca ccagcgtggc atgacgcagg tcgtctttta cagcgccaga
    24661 agggacaaac cacacgtaat cgtattcaac cgtaagttga cgagatagcg aagcagacag
    24721 cgtttcgata caacccgaag gaattttaca gccctggctc tgcactaatg catctgaatg
    24781 ctggcgtggc gcagtgcctt ctggtgatac aacgaccggc cattccagca cccggcttag
    24841 cgttacgttc tcctgaagta gcgggtgatt agggcggaca accagcttca acgattcaag
    24901 aaacagcagt tcgtaattaa gcccggtcat cagttcagga tctgacatcc gaccaatgcc
    24961 gatatcgatt tccccggttt ttaaacccgc cagaatcata gggttactca ttgtcgcaac
    25021 ttgcaaggtc gtctcttttt gttgctgatg aaactgacct ataaccgaag gtaatatccc
    25081 cagtgccgca gtaggtagtg caccaaccct gacgacatca ttattaagac cttctttacg
    25141 atgaagcgac cgtccggcag tgttgatggc gtcaagaact ctgactgcat gcgttaaaaa
    25201 ttgttcgccg ggtaaggtaa gttgcgcccc ctgacgacca cgctcaaaca agcgagcgcc
    25261 agtcagctgc tccagttcat tcaatgtctt agagagcgca ggttgactca aattaagggt
    25321 ttcagccgcg cgccccaaag ttccttgttg tgcgacagct acgaatgtat gaaggtggcg
    25381 caaacggatg cgctgactaa acagactatt tttttccata agcgatgtta aaaacgaagc
    25441 ggtgtcgctg acaagtgaag ttgtttgatt atgataactt gattgcaaaa tattattaac
    25501 aattaaagca attatgttac agcaaaatgg ataatattga tgttttcgcg gcgagatcac
    25561 agtttgtaaa ttcttcccgc aagagtgaat gcggttacct acactccaga ttactgacca
    25621 ctggaggcag acactatggc gaacagcatc acggcggatg agattcggga acagttttcg
    25681 caggcaatgt cagccatgta ccagcaagaa gttccgcagt acggcacgct gctggaactg
    25741 gtagctgatg tgaatctggc tgtgctggaa aacaatcctc aactgcacga aaaaatggta
    25801 aatgcagacg agctggcgcg actgaatgtt gaacgtcatg gggcgattcg cgttgggact
    25861 gcacaagagc ttgctactct tcggcggatg tttgccatta tggggatgta cccggtgagc
    25921 tattacgatc tctcgcaggc aggggtgccg gtacattcga cagcatttcg gcccattgat
    25981 gatgcttctc tggcgcgtaa tcccttccgc gtttttacct ccttactccg ccttgagctt
    26041 atcgagaacg aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc
    26101 accccacgtt gtcgacaact gttagaggaa tatgagcagc agggcggttt taacgaaaca
    26161 caggcacagg aatttgtgca agaggccctg gaaacgtttc gctggcacca gtcagcaacg
    26221 gtagatgaag aaacctatcg cgccttgcac aacgaacatc ggttgattgc tgatgtggtc
    26281 tgttttcctg gatgccatat caaccacctg acgccacgta cgctggatat tgaccgggtg
    26341 cagtcgatga tgcctgaatg cggaattgaa cccaaaattc tgatcgaggg gccgccgcgc
    26401 cgcgaggtac cgattttact acgccagacc agctttaaag cactggaaga gacggtgttg
    26461 tttgcggggc agaaacaggg cacgcatacc gcgcgctttg gtgaaataga gcagcgtggt
    26521 gtggcattaa cgccgaaagg gcgacaactg tatgatgatc ttctgtgtaa cgctggaacc
    26581 gggcaggata atctcactca ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac
    26641 agtgagtttt taatgcgtca gcaagggttg gcatggttcc ggtaccgtct gacgccttcg
    26701 ggtgaggcgc atcgtcaggc gattcatccc ggagacgatc cacagccctt aattgaacgc
    26761 ggttgggtag tggcgcaacc cattacctat gaagatttct tgcccgttag cgcggcgggg
    26821 atcttccagt caaatctggg taatgaaacg cagacacgca gtcacggtaa tgccagtcgc
    26881 gaagcatttg agcaggcgtt gggttgtccg gttttggatg agttccagct ttaccaggaa
    26941 gcggaagaac gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc
    27001 aaaaatttgc ctgattttac aaacgaatca ggctcatcct atcgacataa aaaaaatgcc
    27061 gatttatgca tattctctca gttcaacaat tggattatta ataaatattg tctagagtga
    27121 gcggtcataa ataagcactt tcttgccgct gaaaacgacc agcgcgggac cattcacaac
    27181 accagaagga ctcactttca ggtatggatc gtagacgatt tattaaaggt tcaatggcta
    27241 tggccgccgt gtgcggtacc agcggcattg cttctctttt ttctcaggcg gcattcgcgg
    27301 cagattctga tattgccgac gggcaaaccc agcgttttga cttctccatt ctacagtcaa
    27361 tggcgcacga cttagcgcaa acagcgtggc gtggtgcgcc gcgtccgtta cctgacacgc
    27421 tggcgacaat gacgccgcag gcttataaca gtattcaata cgacgccgaa aaatcgctct
    27481 ggcataacgt tgagaaccgt caactggacg ctcagttctt ccatatggga atgggattcc
    27541 gtcgccgcgt tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt gaaattcact
    27601 ttcgcccgga gttgttcaaa tacaacgatg cgggtgttga taccaaacaa ttagaagggc
    27661 aaagcgatct cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg
    27721 atgtagtatc attcctcggc gcgagttatt tccgtgccgt tgatgataca tatcaatacg
    27781 gtttgtcggc ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa gagttccccg
    27841 actttaccgc cttctggttt gatactgtaa aaccgggggc aactaccttt accgtttatg
    27901 cgttgctcga tagcgccagc attactggtg cctataagtt cactatccat tgtgagaaaa
    27961 gtcaggtgat tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg
    28021 gcattgcgcc gatgaccagc atgttcagct gcggtactaa tgaacgtcgg atgtgcgata
    28081 caattcatcc gcaaattcat gactctgatc gtctgtccat gtggcggggc aacggcgagt
    28141 ggatttgccg tccgctgaat aatccacaaa aattgcagtt caatgcttac accgacaaca
    28201 acccgaaagg gtttggttta ttgcaactgg atcgtgattt ctcccattat caggacatta
    28261 tgggctggta taacaaacgc ccaagtctgt gggtggagcc gcgtaacaag tggggtaagg
    28321 gcaccatcgg cctgatggaa atcccaacaa cgggcgaaac gctggataac attgtctgct
    28381 tctggcagcc agaaaaagct gtaaaagcgg gtgatgagtt tgcattccag tatcgtctgt
    28441 actggagtgc gcaaccgcct gttcattgcc cattagcgcg cgttatggcg acgcgtaccg
    28501 gcatgggcgg tttcccggaa ggctgggcac caggtgaaca ttatcccgaa aaatgggcgc
    28561 gtcgttttgc cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca aaaggcattg
    28621 agccggtgat tacgctttcc agtggggaag cgaagcaaat cgaaattctc tatattgaac
    28681 ccattgatgg ttatcgtatt cagtttgact ggtatccgac ttcggactcc actgatccgg
    28741 tcgatatgcg gatgtatctg cgttgtcagg gggacgctat cagtgaaaca tggctgtatc
    28801 agtatttccc gccagcgccg gataaacgtc agtatgttga cgaccgtgtg atgagttaat
    28861 cgttttttct tcggcacctt ctacgggagg tgccgtctgg ttaaacacga tcccgctcgc
    28921 atttttccct aagttaaatg agtaatctga tggtgtgtat ttcagataca ccttgtcagc
    28981 cactaacagg gagtgcgtat gtttccagaa taccgagatt taatatcccg tctgaaaaac
    29041 gaaaatcctc gctttatgtc cttgttcgat aaacacaata aacttgatca tgaaattgcc
    29101 agaaaggaag gttccgacgg tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa
    29161 aagctacagt taaaagatga gatgctcaaa atcctgcagc aggagagcgt caaagaggtg
    29221 taaactttcc taagccgcca gccaggcggc ttttttaaca actgcatgga ttgactggag
    29281 ataagatgac cgaaacgata aaagtaagtg aatcacttga attacatgct gttgcagaaa
    29341 atcacgtcac tcctctttat cagttaatct gtaaaaataa aacctggtta cagcagtcgc
    29401 taaactggcc gcagtttgtt cagagtgaag aggacacgcg aaaaacggtg cagggtaatg
    29461 tgatgttgca tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa gatgaactta
    29521 tcggcgttat ctcgtttaat cgaattgaac cactgaataa aaccgctgaa atcggctact
    29581 ggctggacga atctcatcag gggcagggga tcatttctca ggcgctgcag gcactgattc
    29641 atcattacgc ccagtctggt gaacttagac gctttgtgat caaatgtcgg gtggacaatc
    29701 cgcaaagcaa tcaggtcgct ttgcgcaatg gttttatcct tgaaggttgc cttaaacagg
    29761 ctgagttcct gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt atcgattcac
    29821 aataaccctg ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca
    29881 cggacatgaa ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt taactccaca
    29941 tggttttcaa ttatcaccgc acctgtaata cggctctcac cttgtatgac aacgtgctcg
    30001 tcgagcagaa tcggtccacc acgtaccacg gcatttccgc caaccaagac atgatgtttt
    30061 aacacacaat taccttccac aatggcatat tccgccacct gcgaactgta atgaatcgtc
    30121 ggaatggcat cttcttctat gccagctttc acctgcgcgt gaccgtagac tttagcgcaa
    30181 tcgcatagcc atacgttgtt ctcttcatta ccttcaacac tggcaaaatc aaaaacttcg
    30241 gcgcgatgtt caataaaagc ataccggacg acggcatcgc cataaatttg tgcctggtgg
    30301 acaatacgcg aggcgctaac ccttgcgcga tcataaattt gtagcaggag ttgatggtcg
    30361 ggcgttaagc cttgtgcggc gacgatcata gaatgttgat caattaaggc gtgaccaaat
    30421 attctacatt gaccgcaaac taatgagcca cgaatggtga cactgtcact tatatgtgca
    30481 ctctggctga tttcactatt atcgatccag acgttatccg ttgcgtaaac ctctccccac
    30541 agcacgctgg tgccggtgat acgcgtatta ccggaaatca ccgttccgcc gaacgcaata
    30601 gcattctggt cgtaaatcca gcaatttcct tcctgtgcta acaccgtttc gcgatcgatc
    30661 cagccgccag cggttcctgc tatcacatcg ttaaaatcgc taatggcgat aatctgccgt
    30721 aataacacat tttttttagt gccatcttcc tgataactaa aggctcgctg ttcttcactt
    30781 agacgatatt tgcgcatagc gttttcccac aggtgactta ctataaccgt agcaaattct
    30841 gcggctctgg ctatgctcga gaaaattcca taaaatgtat ttcaaatata ctttataaat
    30901 taaacaaaat gagtaagaag atgcagagcg ataaagtgct caatttgccg gcaggctact
    30961 ttggtattgt gttggggacg atagggatgg gatttgcctg gcgctatgcc agccaggttt
    31021 ggcaggtcag ccactggtta ggggatgggc tggtgattct ggcgatgatc atctggggat
    31081 tattaactag cgcatttatt acccgactca tacgctttcc gcatagcgtg ctgacggaag
    31141 tgcgccatcc agtgctgagc agttttgtga gtttgtttcc ggcaacgacg atgctggtgg
    31201 cgattggttt tgttccgtgg tttcgcccac tggcggtgtg cctgttcagt tttggtgtcg
    31261 tggttcagtt ggcttatgcc gcctggcaaa ctgcgggatt atggcgcgga tctcaccctg
    31321 aagaagctac cacgcctgga ctgtatctgc cgacagttgc caacaacttt atcagcgcaa
    31381 tggcctgtgg tgcgttgggc tacaccgacg ccggtctggt gtttttaggc gcaggcgttt
    31441 tctcatggct aagccttgaa cctgtgatct tgcagcgtct gcgcagttcg ggagaattac
    31501 ccacggcact gcggacatca ctcggcattc agctcgctcc tgcgctggtg gcctgtagtg
    31561 cctggctgag cgtcaatggc ggcgagggtg acacgctggc gaaaatgctt ttcggttatg
    31621 gactgctgca actgctgttt atgctacgtc tgatgccatg gtatctctcc cagccattta
    31681 atgcttcatt ctggagtttc tcgttcggcg tatctgcact ggcaaccacc ggtttgcatc
    31741 tggggagtgg cagcgataat ggatttttcc atacgctggc ggtgccgctg tttatcttta
    31801 ccaattttat tattgcaata ctgctcatcc gtacttttgc gcttctgatg cagggaaaat
    31861 tgttagtcag aactgagcgc gccgttttaa tgaaagcaga ggacaaagaa tgatcattcg
    31921 tgacgaaaac tattttactg ataaatatga attaacccgc acacactctg aagtactgga
    31981 agcggtgaaa gtggttaaac cgggtaaaac gctggatctg ggctgtggca atggtcgtaa
    32041 cagtctttac ctggcagcca atggttatga tgttgacgca tgggataaaa atgccatgag
    32101 tatcgccaat gtagagcgca ttaaatccat tgaaaatctg gataatttac acacccgagt
    32161 cgttgatctg aataacctca catttgatgg acagtacgat tttattcttt cgactgtggt
    32221 gctgatgttc cttgaggcta aaaccatccc agggctgatt gccaatatgc agcgttgcac
    32281 taaacctggc ggttacaacc tgattgtggc ggcgatggat accgctgatt atccatgtac
    32341 tgtcggcttc ccgtttgcct tcaaagaggg agaattacgt cgatattacg aaggctggga
    32401 gagggtgaaa tacaatgaag acgtcggcga gctgcaccgc accgacgcca acggtaatcg
    32461 tattaaacta cgtttcgcca cgatgctggc acgtaaaaaa tgacccggta agaacaaagc
    32521 gcgtgaaaat tccccacgct gagatggttt actgttcttc ttttcggtaa gcatattttt
    32581 tatcgaagtg atgtgaaatt aatcacagta gtcgaagttt tttatcagct taacatactg
    32641 aaatttaact actgatgatt gacttagccc ctttttcggc attgactatg tcgtctgaaa
    32701 aggggctaaa aaatttattt tcaccaacac tttttttgcc acaacacgaa gcggcgcttt
    32761 ttgctataac ttaaaaagta atataatcat ctcaggaaac tattcatgcg taccacatca
    32821 tttgcgaaag ttgcagcttt atgcggcttg ttggctctgt ctggttgtgc ctctaaaatc
    32881 acccagccag ataaatattc tggtttttta aacaattact ctgatttaaa agaaacaacc
    32941 tcagcaacag gtaaacctgt tttacgttgg gtagacccga gttttgatca aagcaaatat
    33001 gacagcattg tctggaaccc aatcacttat tatccggtac caaaaccttc tacccaggta
    33061 gggcagaaag ttctggataa aattttgagc tataccaaca ccgaaatgaa agaagcgatt
    33121 gcgcagcgta aaccactggt taccaccgct gggccgcgta gtctgatttt ccgtggggcc
    33181 attaccggtg tagataccag caaagaaggg ctgcaattct atgaagtggt tcctgttgca
    33241 ttagtggttg cggggacgca aatggctaca ggccaccgta ccatggatac tcgcctctat
    33301 tttgaaggtg agctgattga tgcagcgact aataaaccgg ttatcaaagt cgttcgtcag
    33361 ggcgaaggta aagacctgaa taacgaaaat acgccaatgg ctttcgaaaa tattaaacaa
    33421 gttattgatg acatggcgac cgatgccacc atgtttgacg ttaacaagaa gtaattccag
    33481 ccgcgccatc gtaagatggc gctttttatc atcggtttct gtcagcgatt aaattcaaca
    33541 ccacgtaaca aacgcctccg gcaattaatc cccagaacgc agaaccaatc ccgaccagcg
    33601 tcaatccact tgccgttacc agaaatgcca ccaccgccgc gtctcgctca cgctcattat
    33661 gcagcgcctg atacaaactg ccgctaatgg tacttaacag cgccagacct gccaacatct
    33721 ggatccaact tacgggcagg gcagccatca tcccggtaat agcactacca aacagacctg
    33781 cgatcaaata aaaaatgcct gcaacggcag ctgccagcca acgttgatct ttatccggat
    33841 gcgcttccgg gctttggcaa atagccgcgg tgattgccgc aataccgacg gaataaacgc
    33901 cgaaagggga aaaaaccagt gccagcaatc cagtaaatac aattaatggc gaaacaggag
    33961 ccgaatatcc ggctgctttc attgctgcga tacccggtgc gttttgcgat gccatcgtca
    34021 ccagaaaaag ggggagtgca acgctcaggc tgtgagcaaa cgaaaaatca ggggtaatat
    34081 aagtggggag aacgggttta aagacaacat catttgtgac aacgtcacct tgcgcgatga
    34141 cgatcacgat cccaataatc atcgcggcaa ttaccgcata gcgcggcgca acggccttgg
    34201 ttgccagcca taccagcaac atactgccac acaacgtaaa ttgaccatcc agactggcaa
    34261 acgcctgtaa accaaagcgt aataaaatcc cggcaagcat tgccgccgca agcgagtgcg
    34321 gaataatgcg catcagacga gcaaagagtc ccgttatgcc gcagaggact attaacgcgt
    34381 tggtgacaat aaaaacgccg atggcttcgt taagtgttag tccctgcaat ccggtgacca
    34441 acaaagccgc gccaggcgtt gaccatgcgg tgagaacagg tacgcgatac cataatgtca
    34501 gagtcagcgt actgacgccc attgccagcc ccagcgccgt catccagcca gagatttgcg
    34561 cagtggtggc tccggcgaca atcgctgctt gccagattat tgccgctgaa ctggcgtagc
    34621 caattaatac cgccagaaac cccgccagta gcgtgggtgg aggaacagaa aacagacgca
    34681 tagtcacctc gtgcgttata gcgtacaggt accgtagcat ttgtccgtta taacgcacaa
    34741 gtgataaact tccgttttgc cggaggagtc gcatggaaaa tctcgctcgc tttttatcca
    34801 ccacacttaa acaactacgc cagcagcgcg gctggagtct ttcgcgattg gcagaagcga
    34861 cgggcgtttc taaagcgatg ttagggcaga ttgagcgtaa tgagtccagc ccgacggtcg
    34921 cgacattatg gaaaattgcc accgggttga acgtgccatt ttcgacattt atttctccgc
    34981 cgcagtccgc aacgccttca gtttatgatc cacaacagca ggcgatggtg atcacttcgc
    35041 tgtttcctta tgatccacaa ctctgctttg aacacttctc gattcagatg gcacccggtg
    35101 caataagcga atcgacgccc catgagaagg gggtaatcga acatgtcgtc gtcattgatg
    35161 gacaacttga tctgtgcgtt gatggtgaat ggcagagcct taattgtggg gaaggcgctc
    35221 gatttgctgc agacgtcacg catgtctatc gtaatggtag ggagcaaatc gtacattttc
    35281 attccctcat ccattacccg cgcagttaag cgggaaaact atttcgcaac gtcgcgcttg
    35341 tggctaaaat agccgccatt tttcagctac tggataagaa tgtgaccgta tcttctcatc
    35401 gacttgaact gttaagcccg gcacgcgatg ccgccattgc ccgcgaagct attttgcacg
    35461 gtgccgatgc tgtttatatc ggcggccctg gttttggtgc ccgtcataat gccagtaata
    35521 gcctgaaaga tattgccgag ctggtgccgt ttgcccatcg ttatggtgca aaaattttcg
    35581 tcacgcttaa caccattttg catgatgatg agctggaacc cgcgcaacgg ctgattactg
    35641 acctctacca gaccggtgtc gatgcgctga ttgttcagga tatggggatt ctggaacttg
    35701 atattccgcc gattgaactg cacgccagta cgcagtgcga cattcgtaca gtagaaaaag
    35761 cgaagttcct ctctgatgtt ggcttcacgc agattgtgct ggcgcgagag ctgaatcttg
    35821 agcagatccg cgcgattcac caggccacgg acgcgaccat tgaattcttt attcatggcg
    35881 cactgtgcgt ggcctattcg ggtcagtgct acatttctca tgcgcaaaca gggcgtagcg
    35941 ccaaccgtgg cgattgctcg caggcgtgcc gtttgccata cacattgaaa gacgatcagg
    36001 ggcgggtggt ttcctatgaa aaacatctgc tgtcgatgaa agataacgat cagactgcca
    36061 acctcggcgc gctgattgat gctggcgtac gttccttcaa gattgaaggg cgttacaaag
    36121 atatgagcta cgtgaagaat atcactgctc attatcgcca gatgctggat gccattattg
    36181 aagaacgtgg cgatctgacg cgtgcttcat caggtcgtac tgaacatttc tttgttccat
    36241 cgacggaaaa gacattccac cgtggtagca cagattattt tgtaaatgcc cgtaaaggcg
    36301 atattggcgc gttcgattcg ccgaaattta tcggcctgcc ggtgggtgaa gtattgaaag
    36361 tggcgaaaga tcatctcgat gttgccgtca ccgagccact ggcaaatggt gatggcctga
    36421 acgtgatgat taaacgtgaa gtcgtcggtt ttcgtgccaa tacggtcgag aaaaccggag
    36481 aaaatcagta ccgcgtctgg cccaatgaaa tgccagcaga tttgcacaaa attcgtccgc
    36541 atcacccact aaaccgtaat cttgatcata actggcagca ggcactgaca aaaacctcca
    36601 gcgaacgtcg ggtggcggta gacattgaac tgggcggttg gcaggaacaa ttgattctga
    36661 ccctcaccag tgaagagggg gtcagcatca cgcatacgct ggatgggcag ttcgacgaag
    36721 ccaataacgc cgaaaaagca atgagcaatc tgaaggatgg tctggcaaaa ctggggcaaa
    36781 ccctctatta cgcccgcgat gtgcaaatta atttgccggg agcgctgttt gtaccaaaca
    36841 gtctgttaaa ccagtttcgc cgtgaagccg ccgacatgct ggatgctgca cgtcttgcca
    36901 gttaccagcg gggcagccgt aaaccggttg ctgatcctgc gccggtttat ccgcaaactc
    36961 atctgagttt cctcgcgaac gtatacaacc agaaagcgcg tgaattttat catcgctatg
    37021 gtgtgcagct gattgacgcg gcgtatgagg cacatgaaga gaagggcgaa gtcccggtga
    37081 tgatcaccaa gcattgtctg cgctttgcct ttaatctgtg cccgaaacag gcgaaaggca
    37141 atatcaaaag ctggaaggcg acgccaatgc aactggttaa cggcgatgaa gtattaacgc
    37201 taaagtttga ttgccgccca tgcgagatgc acgtcattgg caaaatcaaa aatcacatac
    37261 tgaaaatgcc gttaccggga agcgtagtgg catccgtaag tccggatgag ctgctgaaaa
    37321 cattgccgaa gcgaaaaggg taaaacgcca gttttctggt tactcacaac ttattgaatc
    37381 tgcatgatat tgttggccgg gtaaggcgtc acgccgcatc cggcaataaa tgactcaacg
    37441 ttgtcccgct tctggtttgc gcgatttttg ccagtaaaaa tgctcgcgca aaccttccgc
    37501 cgactcttcc gccacagcac gcaattcatc gctgtccgct tcatgacgca gctgatgatc
    37561 cacattcttt acccacacaa attcatgtcc tttgtgccct gccatgagtt gtcctgagaa
    37621 cagcgcacac gttaataaga caaccgataa cgccttcgta aacatcctgc cacctttttc
    37681 ttaccttttg ccgctatgat gccgatcgtt tcttgaggtt attattcagt tttgcaaatt
    37741 agcgcaaaga aattctggaa tcttccttcc tgattttgca ttgcattctg ccgttgcggc
    37801 gatttagtgc tattttcgag caaattacac acggaggtaa acactaatgt ttactttgtt
    37861 gatatactca gcggcaggga ggcgatgtga aacaaagcga gttcagacgt tggctcgaat
    37921 ctcagggcgt cgatgtagcg aatggcagca accatttaaa actcaggttt cacgggaggc
    37981 gcagtgtcat gccgtgtcac cctagcgatg agattaaaga accattgcgt aaagcaattc
    38041 tgaaacaact cggtttgagt taatcgccaa ttaaaaaggt taatgacatg cgagagacag
    38101 tcgaaattat gcgttatccc gtcactctta cacccgcgcc ggaaggcggt tatatggttt
    38161 cttttgtgga tatccctgaa gcgttgaccc agggcgaaac tgtcgctgaa gcgatggaag
    38221 cggcaaaaga tgctttactg accgcatttg atttttattt tgaagataac gagcttatcc
    38281 ctttaccttc gccattaaat agtcatgatc actttattga agtacctttg agcgtcgcct
    38341 ctaaggtatt gctgttaaat gcttttttac agtcagaaat cactcagcaa gagttagcca
    38401 ggcgaattgg caaacctaaa caggagatta ctcgcctatt taacttgcat catgcgacaa
    38461 aaatcgacgt cgtccagctc gcggcaaagg cgcttggcaa agagttatcg ctggtgatgg
    38521 tttaattaca gttaacgaaa agttgtcatt tttaacaact gatatagact gccgaatcat
    38581 ctgcacataa ttacgattcg ataatgaaaa aataccagca gcttgcagaa caattacgcg
    38641 agcagattgc gtcgggtatc tggcaacccg gcgatcgttt gccttcgttg cgtgaccagg
    38701 tggcgctttc tggcatgagc tttatgactg tcagccatgc ctatcagttg ctcgaaagtc
    38761 agggatatat tatcgcacga ccgcagtcag gttattacgt tgcgccacag gcaataaaaa
    38821 tgccgaaagc gccagtcatt ccagtcactc gagatgaagc agtcgatatc aacacttata
    38881 tttttgatat gttgcaggcc agtcgcgatc cgtcggtagt tccgtttgcc tcggcctttc
    38941 ccgacccgcg acttttcccc ctacaacaac taaaccgctc gctggcgcag gtaagcaaaa
    39001 ccgccacggc gatgagcgtg attgaaaact taccgccagg aaacgcagaa ctgcgtcagg
    39061 ctattgctcg tcgctatgcc ttacagggca tcaccatttc tcctgatgaa attgtcatta
    39121 ctgccggggc gttagaggca ttaaacctca gtttgcaagc ggtaactgaa ccgggcgatt
    39181 gggtgatagt agagaatcct tgtttctacg gtgcgttgca ggcgctggag cggctacggc
    39241 tgaaggcgtt atcggtggcg acggatgtta aagaagggat cgatcttcag gcgttggaac
    39301 tggcgttgca ggagtatccg gtgaaagcgt gctggctgat gactaatagc cagaatccac
    39361 tcggatttac cttaacgccg caaaaaaaag cacaactggt ggcgttgctc aatcagtaca
    39421 acgtaacgct gattgaagat gacgtttaca gcgaacttta ttttggacgg gaaaaaccgc
    39481 tgcctgcgaa agcgtgggat cgccacgatg gcgttttgca ttgctcttcg ttttcgaaat
    39541 gtctggtgcc tggttttcgt attggttggg tcgccgccgg aaaacatgca cgtaaaattc
    39601 aacgcttgca gttgatgagt acgctttcca ccagctcacc gatgcagctt gcgctggtgg
    39661 attacctttc cacgcgccga tacgacgccc atcttcgtcg cctgcgtcgc cagcttgcgg
    39721 aacgtaaaca acgtgcctgg caggcactgc tgcgttatct gcctgcggaa gttaaaattc
    39781 atcataatga cagcggttat tttctctggc tggagctccc cgagccgtta gatgccggcg
    39841 aattaagcct ggcggcactg acgcatcata tcagtattgc gccgggtaaa atgttttcta
    39901 ccggtgaaaa ctggtcacgt ttttttcgtt ttaataccgc gtggcagtgg ggagaacgtg
    39961 aagaacaggc ggtaaaacaa ttaggcaaac ttattcaaga acggctgtaa tagcgtttaa
    40021 tttaattcct cttagattgg gtaatatgaa tttcgaatag cagtcatatt tcctaactcc
    40081 ttgtctatac tccagaagat aaccttacag acggcataat gcgcggtagc tcacaacctg
    40141 attaaatttt ctcaggggcg aaggtgtgcc tgcaagccgc cgtctatggt taaacaagga
    40201 gatattttta cggcacggcg gctgaacaat taattacgac aggagtaaga ctttatgagc
    40261 aagacatttg cccgcagcag cctgtgtgcg ctcagcatga caataatgac cgctcacgcc
    40321 gccgaaccgc ctaccaattt agataaaccg gaagggcgac tggatattat cgcctggccg
    40381 ggatatatcg aacgcggaca aactgataaa caatacgact gggtaacgca attcgaaaaa
    40441 gagacaggct gcgcggtgaa tgtgaaaacc gccgcgactt ccgatgaaat ggtcagtctg
    40501 atgaccaaag ggggttacga tctggttact gcatccggcg atgcctcgct gcgcctgatt
    40561 atgggtaaac gcgtgcagcc gattaatacc gcattgatcc ccaactggaa aacgctcgat
    40621 ccccgcgtgg ttaaaggcga ctggttcaat gttggcggca aagtttacgg cacaccatac
    40681 cagtgggggc cgaatctgct gatgtacaac actaaaacct tcccgacgcc gccggatagc
    40741 tggcaagtgg tttttgttga gcaaaatctg ccggacggca agagcaataa aggccgcgtt
    40801 caggcttatg atggcccgat ctatattgcg gacgctgcgt tgttcgttaa agccactcag
    40861 ccgcagttgg gcatcagcga tccgtatcaa ctcaccgaag aacagtacca ggcggtgctg
    40921 aaagtgctgc gggatcaaca tagtttgatc catcgctact ggcatgacac taccgtgcaa
    40981 atgagcgatt tcaaaaacga gggtgtggtt gcttccagtg catggcccta tcaggccaac
    41041 gccctgaaag ccgaaggcca gcctgttgct accgttttcc cgaaggaggg tgttaccggt
    41101 tgggctgata ccaccatgct gcatagcgaa gcgaaacatc cggtttgcgc ctacaaatgg
    41161 atgaactggt cattaacgcc aaaagtacag ggcgatgtgg cggcctggtt tggctcgtta
    41221 ccggtagtgc cggaagggtg taaagccagt ccgttattag gcgaaaaagg ttgtgaaaca
    41281 aacggtttta actatttcga caaaatcgcc ttctggaaaa cgcctatagc agaagggggc
    41341 aagtttgttc cctacagtcg ctggacgcag gattacattg ccattatggg cggtcgctaa
    41401 cttcgctggg gtgctttatg acgtacgcag tggagtttga caacgtctcg cggttgtacg
    41461 gtgacgtgcg cgcagtagat ggcgtcagta ttgcgataaa agatggtgag ttcttctcta
    41521 tgctggggcc gtccggctcc ggcaaaacca cctgcctgcg cctgattgct ggcttcgaac
    41581 agctttccgg cggggctatc tctatctttg gtaaacccgc cagcaatctg ccaccgtggg
    41641 agcgggacgt gaatactgtc tttcaggact acgcgctatt tccgcatatg tcgattcttg
    41701 acaatgtcgc ctatgggctg atggtcaaag gcgtgaataa aaagcagcgg cacgcaatgg
    41761 cgcaagaggc gctggagaaa gtggcgttgg ggtttgtaca tcaacgtaaa ccgtcacaac
    41821 tttctggtgg tcagcgccag cgggttgcta tcgccagagc attggtgaat gaaccgcgcg
    41881 tattgctgtt ggatgaaccg ctcggcgcac tggatctcaa attgcgtgag cagatgcagc
    41941 tggaactgaa aaaactgcaa cagtctctcg gtatcacttt tatcttcgtt acccacgatc
    42001 agggcgaagc gttatcgatg tccgatcgtg tggcggtttt caataatgga cgcattgagc
    42061 aggtcgattc cccgcgcgat ctctatatgc gcccgcgcac gccgtttgtt gccgggttcg
    42121 ttggtacatc gaatgttttt gatggactga tggcagagaa actttgtggc atgacgggaa
    42181 gcttcgccct gcgaccggaa catatccgcc tcaacacccc tggtgaactg caggccaatg
    42241 gcacgatcca ggcggtgcaa tatcagggcg cggcaactcg ttttgaactg aaattgaacg
    42301 gcggtgaaaa actgcttgtg agtcaggcca atatgacagg cgaagaactg cctgccacgc
    42361 tcacgcccgg acaacaggtg atggtttcct ggtcgcgtga tgtgatggtg ccgctggttg
    42421 aggagaggtg aatggcgatg aatgtattgc aatcaccttc acgtccaggt ctgggtaagg
    42481 tgtccggttt cttctggcgt aatccggggc tggggctgtt tttactgctg cttggcccgc
    42541 taatgtggtt tggcattgtc tatttcggct cgctgctgac actgttatgg cagggatttt
    42601 atacttttga cgatttcacc atgtcggtaa cgccggaact gacgctggcg aatatccgtg
    42661 cgctgtttaa tccggcgaat tacgacatca ttctccgcac gctgactatg gctgtggcag
    42721 tcactatcgc cagcgccatt ctggcttttc caatggcgtg gtatatggcg cgctatacca
    42781 gcgggaaaat gaaagcgttt ttttatattg cggtaatgtt gccgatgtgg gcgagctaca
    42841 ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct cagtggtttt
    42901 tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct gcggtgggcg
    42961 gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat atctggttgc
    43021 cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca ttgttgcagg
    43081 cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg ctgccgctgg
    43141 caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg ggcgatttta
    43201 tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt tattcccagc
    43261 agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg attattctca
    43321 tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc tgaacgcgca
    43381 ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt tcccatcctg
    43441 ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc accgcagggc
    43501 ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga tgccgtgaca
    43561 ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg gacgctggca
    43621 gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct gttactgctg
    43681 ctgcccattg cgctgccggg cattgtcact ggtctggcgt tattaaccgc ctttaaaacc
    43741 atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt ttgtgtagtt
    43801 gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct ggttgaggcg
    43861 tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt gccgaatctc
    43921 agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga tgaaatcatc
    43981 gttacgacct ttacggcagg tcatgaacga acgttaccgt tgtggttgct caatcagctt
    44041 gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat gttggtaaca
    44101 accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg tcaataacca
    44161 ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact ggttagcggc
    44221 gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact ggaaattgcc
    44281 gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc atttgccgaa
    44341 tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc tgatgttatc
    44401 gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa accgctgcat
    44461 agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt cgcgggtgcg
    44521 gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac ttcgatgatc
    44581 cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc gctgatgatg
    44641 gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct taaaccatca
    44701 gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat cttcccggca
    44761 ggcgtgatta acgtactgtt tggcagaggc aaaacggtgg gggatccgct gaccggtcat
    44821 cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca catcatcagc
    44881 cataccgcgc cctccattaa gcgtactcat atggaacttg gtggcaaagc gccagtgatt
    44941 gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt tggctattac
    45001 aatgccggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg catttacgat
    45061 acgctggtgg aaaaactcgg tgctgtggtg gcaacgttaa aatcgggtgc gccggatgac
    45121 gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt cagcaaggcg
    45181 gtagaagagg cgaaagcgac agggcatatc aaagtgatca ccggcggtga aaagcgcaag
    45241 ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga cgatgccatc
    45301 gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc tcttcgacaa cgaagaacag
    45361 gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg gactaaagat
    45421 gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg ggtcaatacc
    45481 catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg ttacggcaag
    45541 gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat ggttaaacat
    45601 taaggataat attgcagatc gtaagagtat gagatgatct tgcgtactgt cagagagttg
    45661 atttctctga cagtacgtgc tgcgtgcaaa atcactacat caaataaacc agccaaatct
    45721 catagagata agagtaaaca aaccaacagc agcaaagaca tttatcagta ctacagttct
    45781 actggaaaca ttcatttttg tcacctttta tcattgcccc tatgtgtata gtacgggtta
    45841 agaaaatccg caatcatact cattctgaag atggataagg gcaagttgct gtttgatgtt
    45901 tttttctcaa accggtgaaa aaacttgatg cacgtcaaaa aatgacgcat atttgcgcgc
    45961 gttttattca tctggctgga cgcccgtaca tgtctctcta tcaacacatg cttgtttttt
    46021 atgcggttat ggcagcaatc gcatttctta tcacctggtt tctttctcac gataagaaac
    46081 gcatccgttt cttaagcgcc tttctggtgg gggcaacatg gccgatgagt ttcccggtgg
    46141 cgctgttgtt ttcactgttt taaatcttac tcactggctt gacggcacca cacagactta
    46201 tatcatttgg atgaatcgat aaatttcaca agtggctaag gagaaagtat gtcgcatctg
    46261 gatgaagtca tcgcgcgcgt ggatgccgcg attgaagaga gcgtcattgc ccatatgaac
    46321 gaattattaa tcgccctgag cgatgacgcg gagttaagtc gggaagatcg ctacacccag
    46381 cagcagcgct tgcgcaccgc tattgcccat cacggtcgca agcataaaga agatatggaa
    46441 gtgcgccacg aacagttaac caaaggcggc accatcctct gattaaaatg agcgtctggc
    46501 caccaaccag gcgccaacca caagtaaaat tgcgccacaa attgggccga acaacgcccg
    46561 tagtggtacg ccatgactgc ggaagatatc cagaaccaga ccgccaatca gctggctggc
    46621 gaccagtact gcaatagttg tcgccgctcc cacattctga tatccgctga tactggcgaa
    46681 gacaaaaaac gatcccagca agccaggtat aagtgtccac cagcg
//
LOCUS       DBBGLQ010000033        43723 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.033, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000033 DBBGLQ010000000
VERSION     DBBGLQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.033"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..239)
                     /locus_tag="ACIYSB_002405"
     CDS             complement(<1..239)
                     /locus_tag="ACIYSB_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HGC5429443.1"
                     /translation="MNEAYKILVNNALERYHFRLAELGVSEDSTARELAEQAIYGLFM
                     HAFASDDKVAQEELRLIVAELFNGSAVIEPYFIERA"
     gene            complement(252..546)
                     /locus_tag="ACIYSB_002406"
                     /pseudo
     CDS             complement(252..546)
                     /locus_tag="ACIYSB_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
     gene            complement(546..761)
                     /locus_tag="ACIYSB_002407"
     CDS             complement(546..761)
                     /locus_tag="ACIYSB_002407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429444.1"
                     /translation="MTPRQKLTTSNNDVLICSNKHVVAATKVGSLITIHFANFSLEIQ
                     TNNEGEAAAYMNVLKNSLREKRNTEVQ"
     gene            complement(792..1070)
                     /locus_tag="ACIYSB_002408"
     CDS             complement(792..1070)
                     /locus_tag="ACIYSB_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGC5429445.1"
                     /translation="MTKVLNTYGETDFNKLAAMYPYRDEHGLPVLEESLEDYAKRTNQ
                     ALNTVKKQVDRAQIPINQHKKGGKRTVNLYAIFLKTIINAEKYVLMTK"
     gene            1168..1476
                     /locus_tag="ACIYSB_002409"
     CDS             1168..1476
                     /locus_tag="ACIYSB_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5429446.1"
                     /translation="MFIGEKIRVIRESEGLTREEFCELIDVPIGTLRRYETGRIENVG
                     GEALVKIVNHPRFFKYMNWLMTGKTNEAAGQISPTLSPDGPESTSPSQKVRKTGTLHG
                     "
     gene            1565..2497
                     /locus_tag="ACIYSB_002410"
     CDS             1565..2497
                     /locus_tag="ACIYSB_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5429447.1"
                     /translation="MRPDGRKGKRIRKKFKTKSEAIQFERWVLAQHQNNAWNSVPTDR
                     RSLSELIELWWKFHGQLMKSGWNTRLKLQRVCEAMGDPRVNNVNATTLTELRVARAEE
                     GIQPSTINREVGALSAMFSALISSGHFQNDNPVSGLKGMKVSEREMGYLNRQECLQLL
                     DVLVEHPDERLATEILLSTGARWGEVVELEQRRVLHSRITFSRTKNGKNRTVPISESL
                     FDKIKKRGGKLVFPALDYSLVRDSIKAVAPSIPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVKLNPIA"
     gene            2509..2892
                     /locus_tag="ACIYSB_002411"
     CDS             2509..2892
                     /locus_tag="ACIYSB_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429448.1"
                     /translation="MNDSERQGEMEKKKREFIKKMESITPRQFFRFLDEKNVTVVCPG
                     CGLKDTQITATTGKLNLQQLMDGEKGEEFMTYFRLEPGHPGDSDANYYYKSFCENCGY
                     ITMHAVTPVLNWLGSQKNQEGSGDE"
     gene            2885..3184
                     /locus_tag="ACIYSB_002412"
     CDS             2885..3184
                     /locus_tag="ACIYSB_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429449.1"
                     /translation="MNEYTYIAGFSIPYPGGVQVSNRRSQASRDEFALDSRVARMESD
                     ISYIKRDIEELKSDLKGVRGDIDKIRTTDFRFMFGALITTALGLAGLMARGFHWI"
     gene            3376..4356
                     /gene="btuC"
                     /locus_tag="ACIYSB_002413"
     CDS             3376..4356
                     /gene="btuC"
                     /locus_tag="ACIYSB_002413"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGC5429450.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            4419..4970
                     /gene="btuE"
                     /locus_tag="ACIYSB_002414"
     CDS             4419..4970
                     /gene="btuE"
                     /locus_tag="ACIYSB_002414"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGC5429451.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKFAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWVDRGFMVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            4970..5719
                     /gene="btuD"
                     /locus_tag="ACIYSB_002415"
     CDS             4970..5719
                     /gene="btuD"
                     /locus_tag="ACIYSB_002415"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGC5429452.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            5797..6261
                     /gene="nlpC"
                     /locus_tag="ACIYSB_002416"
     CDS             5797..6261
                     /gene="nlpC"
                     /locus_tag="ACIYSB_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGC5429453.1"
                     /translation="MRFCLILITALLLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            6508..7221
                     /gene="ydiV"
                     /locus_tag="ACIYSB_002417"
     CDS             6508..7221
                     /gene="ydiV"
                     /locus_tag="ACIYSB_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGC5429454.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            7284..8720
                     /gene="selO"
                     /locus_tag="ACIYSB_002418"
     CDS             7284..8720
                     /gene="selO"
                     /locus_tag="ACIYSB_002418"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGC5429455.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(8724..8915)
                     /gene="hemP"
                     /locus_tag="ACIYSB_002419"
     CDS             complement(8724..8915)
                     /gene="hemP"
                     /locus_tag="ACIYSB_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGC5429456.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(9047..10093)
                     /gene="aroH"
                     /locus_tag="ACIYSB_002420"
     CDS             complement(9047..10093)
                     /gene="aroH"
                     /locus_tag="ACIYSB_002420"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGC5429457.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(10250..11083)
                     /gene="ppsR"
                     /locus_tag="ACIYSB_002421"
     CDS             complement(10250..11083)
                     /gene="ppsR"
                     /locus_tag="ACIYSB_002421"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGC5429458.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            11416..13794
                     /gene="ppsA"
                     /locus_tag="ACIYSB_002422"
     CDS             11416..13794
                     /gene="ppsA"
                     /locus_tag="ACIYSB_002422"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGC5429459.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(13851..15497)
                     /gene="fadK"
                     /locus_tag="ACIYSB_002423"
     CDS             complement(13851..15497)
                     /gene="fadK"
                     /locus_tag="ACIYSB_002423"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HGC5429460.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMSDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTTSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(15554..15847)
                     /gene="ydiT"
                     /locus_tag="ACIYSB_002424"
     CDS             complement(15554..15847)
                     /gene="ydiT"
                     /locus_tag="ACIYSB_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGC5429461.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAENPDINEFHKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(15844..17133)
                     /gene="ydiS"
                     /locus_tag="ACIYSB_002425"
     CDS             complement(15844..17133)
                     /gene="ydiS"
                     /locus_tag="ACIYSB_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGC5429462.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(17189..18127)
                     /gene="ydiR"
                     /locus_tag="ACIYSB_002426"
     CDS             complement(17189..18127)
                     /gene="ydiR"
                     /locus_tag="ACIYSB_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGC5429463.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCLYVLAQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(18147..18911)
                     /gene="ydiQ"
                     /locus_tag="ACIYSB_002427"
     CDS             complement(18147..18911)
                     /gene="ydiQ"
                     /locus_tag="ACIYSB_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGC5429464.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            19227..20138
                     /gene="ydiP"
                     /locus_tag="ACIYSB_002428"
     CDS             19227..20138
                     /gene="ydiP"
                     /locus_tag="ACIYSB_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5429465.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(20181..21332)
                     /gene="ydiO"
                     /locus_tag="ACIYSB_002429"
     CDS             complement(20181..21332)
                     /gene="ydiO"
                     /locus_tag="ACIYSB_002429"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGC5429466.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21346..22941)
                     /gene="ydiF"
                     /locus_tag="ACIYSB_002430"
     CDS             complement(21346..22941)
                     /gene="ydiF"
                     /locus_tag="ACIYSB_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGC5429467.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(23084..23842)
                     /gene="aroD"
                     /locus_tag="ACIYSB_002431"
     CDS             complement(23084..23842)
                     /gene="aroD"
                     /locus_tag="ACIYSB_002431"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGC5429468.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(23873..24739)
                     /gene="ydiB"
                     /locus_tag="ACIYSB_002432"
     CDS             complement(23873..24739)
                     /gene="ydiB"
                     /locus_tag="ACIYSB_002432"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGC5429469.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQADCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(24751..26016)
                     /gene="ydiN"
                     /locus_tag="ACIYSB_002433"
     CDS             complement(24751..26016)
                     /gene="ydiN"
                     /locus_tag="ACIYSB_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5429470.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVTNELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIILDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFCTRLNTIKHRG"
     gene            complement(26243..27457)
                     /gene="ydiM"
                     /locus_tag="ACIYSB_002434"
     CDS             complement(26243..27457)
                     /gene="ydiM"
                     /locus_tag="ACIYSB_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5429471.1"
                     /translation="MKNPYFPTALGLYFNYLVHGMGVLLMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(27557..27913)
                     /gene="ydiL"
                     /locus_tag="ACIYSB_002435"
     CDS             complement(27557..27913)
                     /gene="ydiL"
                     /locus_tag="ACIYSB_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGC5429472.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(28049..28156)
                     /gene="rprA"
                     /locus_tag="ACIYSB_002436"
     ncRNA           complement(28049..28156)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACIYSB_002436"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(28342..29454)
                     /gene="ydiK"
                     /locus_tag="ACIYSB_002437"
     CDS             complement(28342..29454)
                     /gene="ydiK"
                     /locus_tag="ACIYSB_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGC5429473.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            29843..32899
                     /gene="ydiJ"
                     /locus_tag="ACIYSB_002438"
     CDS             29843..32899
                     /gene="ydiJ"
                     /locus_tag="ACIYSB_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGC5429474.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFRYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            32896..33306
                     /gene="menI"
                     /locus_tag="ACIYSB_002439"
     CDS             32896..33306
                     /gene="menI"
                     /locus_tag="ACIYSB_002439"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGC5429475.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            33406..33594
                     /gene="ydiH"
                     /locus_tag="ACIYSB_002440"
     CDS             33406..33594
                     /gene="ydiH"
                     /locus_tag="ACIYSB_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGC5429476.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            34142..34510
                     /gene="sufA"
                     /locus_tag="ACIYSB_002441"
     CDS             34142..34510
                     /gene="sufA"
                     /locus_tag="ACIYSB_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGC5429477.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            34519..36006
                     /gene="sufB"
                     /locus_tag="ACIYSB_002442"
     CDS             34519..36006
                     /gene="sufB"
                     /locus_tag="ACIYSB_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGC5429478.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            36016..36762
                     /gene="sufC"
                     /locus_tag="ACIYSB_002443"
     CDS             36016..36762
                     /gene="sufC"
                     /locus_tag="ACIYSB_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGC5429479.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVHPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            36737..38008
                     /gene="sufD"
                     /locus_tag="ACIYSB_002444"
     CDS             36737..38008
                     /gene="sufD"
                     /locus_tag="ACIYSB_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGC5429480.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLINSQFVSIAGEISPQQRDALALTLDSVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENSLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            38005..39225
                     /gene="sufS"
                     /locus_tag="ACIYSB_002445"
     CDS             38005..39225
                     /gene="sufS"
                     /locus_tag="ACIYSB_002445"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGC5429481.1"
                     /translation="MIFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            39238..39654
                     /gene="sufE"
                     /locus_tag="ACIYSB_002446"
     CDS             39238..39654
                     /gene="sufE"
                     /locus_tag="ACIYSB_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGC5429482.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            39803..40807
                     /gene="ldtE"
                     /locus_tag="ACIYSB_002447"
     CDS             39803..40807
                     /gene="ldtE"
                     /locus_tag="ACIYSB_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGC5429483.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRVTQ
                     "
     gene            complement(40871..41107)
                     /gene="lpp"
                     /locus_tag="ACIYSB_002448"
     CDS             complement(40871..41107)
                     /gene="lpp"
                     /locus_tag="ACIYSB_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGC5429484.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(41418..42830)
                     /gene="pykF"
                     /locus_tag="ACIYSB_002449"
     CDS             complement(41418..42830)
                     /gene="pykF"
                     /locus_tag="ACIYSB_002449"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGC5429485.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(42838..43032)
                     /locus_tag="ACIYSB_002450"
     CDS             complement(42838..43032)
                     /locus_tag="ACIYSB_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429486.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            43387..43596
                     /gene="fumD"
                     /locus_tag="ACIYSB_002451"
     CDS             43387..43596
                     /gene="fumD"
                     /locus_tag="ACIYSB_002451"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGC5429487.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT        11031 a        10509 c        11192 g        10991 t
ORIGIN      
        1 gccctctcta taaaataagg ctcgataact gcggaaccgt taaacaattc cgccacaata
       61 agacgcaatt cttcctgcgc cactttgtca tcgctggcga acgcgtgcat aaataaaccg
      121 taaattgcct gttctgccag ttcgcgagct gtcgaatctt cgcttacgcc cagctcagcc
      181 agcctgaagt gataacgttc aagcgcatta ttaaccagta ttttataggc ttcgttcatt
      241 gcggcctcga cttattgctc tggatgctga aagctgcgcc ccgcaattaa gcggtcggca
      301 gttcgcaagg cttcgtgtag cgtgaaatcc tgcccgaact gtcagtgcaa agatgcggtt
      361 tccggtaaac gggttgcggt tgcatctgtg gatcacgagt ccagcctgct caatcaacca
      421 ggtgtgttcg ccagtttgct ttacaacgtg accgcccggc gttgcgtgtg tttcgcgcag
      481 gctccagcgg ttattgctgc gtggcacact gttacggata tatggttgtg aataatgttt
      541 tcgcattatt gcacctcggt gtttcttttt tctcgcaggg agtttttcaa tacattcatg
      601 tatgctgcgg cttctccctc gttattggtc tgtatctcca ggctgaaatt agcaaagtga
      661 atagtgatta atgagccgac ctttgttgca gccactacgt gtttatttga acagattaat
      721 acatcattat tgctggtagt taatttttgt cttggggtca tgttgttgcc ctcgttattt
      781 gcggaaaata gttatttcgt catgagtaca tatttctctg cgtttatgat ggttttcaga
      841 aaaattgcgt agagatttac tgtgcgtttc ccgccttttt tatgctgatt tatagggatc
      901 tgcgctctgt cgacctgttt tttaacagtg ttcagggctt gattggttcg tttggcgtag
      961 tcctcaaggc tttcctcaag aactggcagt ccatgctcat cacggtaggg gtacattgcc
     1021 gccagcttgt tgaagtccgt ctccccgtat gtgttcaaga cttttgtcat gatgtggtaa
     1081 cctttccagt ctgtggctat ttgtattctt gtgtacttat aagtacacct tgaaatcaag
     1141 aatactgtac tgataggaac actgtcaatg tttattggtg aaaaaatcag agtaattcgt
     1201 gagtcggaag ggttaacgcg tgaagagttt tgtgaactga ttgatgtacc tatcggtact
     1261 ctacgccgtt atgagacagg tcggattgag aatgtcggcg gcgaagctct cgtcaagatt
     1321 gttaatcatc cccgtttttt taagtacatg aattggctca tgacgggaaa aacaaacgag
     1381 gctgctgggc agatcagccc gactctctcc cctgatgggc cagaaagcac atcaccttct
     1441 caaaaagtcc gcaagactgg aacactgcac ggctgattat ggaacgctgg gggcacggtg
     1501 ggcttgtgac gctgggggta tgccaatgag cataaaatca atccctggtg gctacatggt
     1561 tgatatgcgt cctgatgggc gcaaaggtaa acgcattcgt aaaaagttca aaacaaagtc
     1621 ggaggctatc cagttcgaac gctgggtttt agctcaacac caaaataacg catggaacag
     1681 tgtgccgacc gatcgccgtt cgttgtccga acttatcgaa ttatggtgga agtttcacgg
     1741 gcaactgatg aaatcggggt ggaacacacg cctcaaatta cagcgcgtgt gtgaagcgat
     1801 gggggatccg cgtgttaaca acgtcaacgc caccacgtta acggaattac gcgtggcaag
     1861 agcggaggag gggattcagc caagtaccat aaaccgcgag gttggtgcgc ttagcgcgat
     1921 gttttccgca ctcatctcat ccggtcactt ccagaatgat aaccctgtta gcggactgaa
     1981 agggatgaag gtcagcgagc gcgaaatggg gtatctgaac aggcaggagt gcctccagct
     2041 gcttgacgtt ctggtagagc atcccgacga acggctggct actgaaattc ttttatccac
     2101 cggggcgcga tggggtgagg tggtggaact tgaacagcgc cgcgttctgc attcccggat
     2161 cacattttcc aggaccaaaa acggtaaaaa ccggactgtt ccaatttctg aaagcctgtt
     2221 tgacaaaatt aaaaaacggg gcggaaagct ggtatttccg gcgctggatt attcgctggt
     2281 gcgcgattcg ataaaagcgg ttgccccttc cattcctgac gggcaggccg ttcacgcgct
     2341 gcgccacact ttcgccagtc atttcatgat gaacggcggc aatattctga cgctccagaa
     2401 aattctgggg cacgccaaga ttcagacaac gatgatttat gcccatcttg caccggatta
     2461 tttgcaggat gcggtgaaat taaatcctat agcttaaagg ttgcgaaaat gaacgattcc
     2521 gaacgtcaag gggaaatgga aaagaaaaaa cgggagttta taaaaaaaat ggaatcgata
     2581 acgccaaggc aattcttccg ttttttagat gaaaaaaacg tgacggtggt ttgccctggg
     2641 tgcgggctga aagataccca gataactgcc actacaggta agttaaatct acaacaactc
     2701 atggatggtg aaaaagggga agagtttatg acgtacttca ggcttgaacc tggtcacccg
     2761 ggtgatagtg atgcgaatta ttactacaaa tctttttgtg agaactgcgg ttatataact
     2821 atgcatgcag taaccccggt attgaactgg ctgggatcgc aaaaaaacca ggaagggtca
     2881 ggtgatgaat gagtatactt atatcgcggg tttttccatt ccgtatccag gaggagttca
     2941 ggtgagcaat cgcagaagtc aggcaagtcg tgatgagttc gctcttgata gtcgtgttgc
     3001 acgaatggaa tctgatatct catacatcaa gagagatatt gaggagctga agagcgacct
     3061 caagggtgta agaggcgata ttgataagat acgcacaaca gatttcaggt tcatgtttgg
     3121 tgcgttaatt actacagcct tggggttagc tggcctaatg gcgagaggtt ttcactggat
     3181 ttaagatcca caaagtgacc acatccctgt tatttgttgt ggttggctgt gtttttgtgt
     3241 gtctgtaagt ctttgataat tatctaactt attgattttt acttgtgttt atggccgctc
     3301 tgcggccttt tttcttttca ctgtcgaaga gtcaccgtaa aatcaacgcc atgacacttc
     3361 agcagaacgg ataccatgct gacacttgcc cgccaacaac agcgacaaaa tattcgctgg
     3421 ttattatgcc tgtcagtttt gatgctgctg gcgcttctct taagcctttg cgccggtgaa
     3481 caatggatct cgccaggtga ctggtttact cctcgtggcg aactgttcgt ctggcaaatt
     3541 cgcctgccac gtacgctggc tgtattgctg gttggtgcgg cgctggctat atccggcgct
     3601 gtaatgcagg cgttgtttga aaatcctctg gcagaacctg gactacttgg cgtctctaac
     3661 ggcgcaggcg tggggcttat cgccgcggta ttgcttgggc aagggcaact ccccaactgg
     3721 gcgctagggc tgtgtgcgat tgctggcgcg cttatcatca ctttaatact cttacgtttc
     3781 gcccgtcgtc atctttcgac cagtcggtta ttgctggctg gcgttgcatt agggattatc
     3841 tgtagcgcac taatgacgtg ggctatctac ttttccacct cagttgattt gcgtcagctg
     3901 atgtactgga tgatgggcgg ttttggcggc gtagactggc ggcaaagctg gctgatgctg
     3961 gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca ggccgatgaa tatgttagca
     4021 cttggcgaga tctcggcgcg gcaactgggt ttacccctgt ggttctggcg caatgtgctg
     4081 gtggcagcga ccggctggat ggttggcgtc agtgtggcgc tggcgggtgc tatcggcttt
     4141 attggtctgg tgatccccca tattctccgg ttgtgtggtt taaccgatca tcgcgtatta
     4201 cttcccggct gcgcgctggc aggggcgagc gcattgctgc tggccgatat tgtagcgcgc
     4261 ctggcattag ctgccgcaga gctgcctatt ggcgtggtca ccgcaacgtt aggtgcgccg
     4321 gtgtttatct ggttattgtt aaaagcagga cgttagccgc aaaaagacgg tctatgatta
     4381 aaagctaaga tttttactga ccacacccag gagaaacgat gcaagattcc attctgacga
     4441 ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga gaagttcgcc ggtaatgtgc
     4501 tgttgattgt caatgtcgcc tcaaagtgtg gcttaacgcc gcaatatgag cagttggaga
     4561 atattcagaa agcctgggtc gatcgaggtt ttatggtgct gggattcccg tgcaaccagt
     4621 ttctggaaca agaaccgggc agcgatgaag agattaaaac ttactgtacc accacatggg
     4681 gggtgacgtt cccgatgttc agtaagattg aagttaatgg cgaaggacgc catccgctgt
     4741 atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc ggaagagagc ggattctatg
     4801 cccgtatggt cagcaaaggc cgtgcaccgc tgtacccgga tgatatttta tggaattttg
     4861 aaaaattcct ggttggcagg gacggaaaag tcatccagcg tttttccccg gatatgacgc
     4921 cggaagatcc cattgtgatg gaaagcatta aactggcgtt ggcaaaataa tgtctattgt
     4981 gatgcagtta caagatgttg cggaatctac ccgcctgggg ccgctttctg gcgaggttcg
     5041 ggctggggag atcctgcacc tggtggggcc gaatggcgcg ggtaagagta ccttactggc
     5101 gcgaatggcc ggaatgacca gcggtaaggg aagcattcag ttcgcggggc aaccactgga
     5161 agcatggtcc gcaacaaaac tcgcgctgca tcgcgcctat ctttcacaac agcagacgcc
     5221 gccgtttgca atgccggtct ggcactacct gacactgcat cagcacgata aaacgcgtac
     5281 cgaactactg aatgatgtcg caggggcgct ggctcttgat gacaaactcg gacgtagcac
     5341 caatcaactt tccggcggtg aatggcaacg cgtacgtctt gctgcggtgg tgttgcaaat
     5401 cacaccacaa gccaatcccg caggccaatt gctgcttctt gatgagccga tgaacagtct
     5461 tgatgttgcg caacaaagtg cgttagacaa aattctgagc gcgctgtgtc agcaaggact
     5521 ggcgattgtg atgagcagtc acgatctcaa ccacacattg cgtcatgcgc atcgggcgtg
     5581 gttgctaaaa ggtggaaaaa tgctggccag tggacgcagg gaagaggtgc tcacgccgcc
     5641 aaatctggcg caggcctatg ggatgaattt tcgccgtctg gatatcgaag gtcacagaat
     5701 gctgatttcg accatctgat ttcattagta tcttgaaaat cgggtaatta cgacgctaaa
     5761 ttaatgcaag aataaaaaac agaggaaccg tacggaatgc gtttctgcct tattttgatc
     5821 acagcactgc ttctggccgg gtgtagccac cataaagcac cgccgccaaa tgccagactt
     5881 tctgattcga ttaccgttat tgccggtttg aacgaccagc tacaaagctg gcatggcacg
     5941 ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact gttcgggatt tgtggttgtg
     6001 acgatgcgcg atcgtttcga tttgcagctg ccccgtgaaa ccaaacaaca agcctctatt
     6061 ggcacgcaaa ttgataaaga cgagttgctg ccaggtgacc tggtcttttt caaaacgggg
     6121 tccggacaaa atggtttgca tgtaggtatt tacgatacca acaaccaatt tatccacgcc
     6181 tctaccagca agggagtgat gcgttcctca cttgataatg tctattggca gaaaaatttc
     6241 tggcaggcga gacgaatcta gtatgtcgaa aaatggacgg cgatgataca tcgccgtcca
     6301 tttactcctg aatacagtag tattttgcag acaaaaattc cgtaaatgga ttattcgttt
     6361 ataatcagga taatctaaaa aaaggacccc tgaaaaagtg gttaacatgg aacaaatgaa
     6421 agttactcct tctgtaattg ttatgtctta tggcctcatc ccttttagcc ggatactgaa
     6481 aaacatcctt cgagagggac ggttaccatg aagatttttt tggagaatct ttatcattct
     6541 gattgctatt tcctgccgat aagagacaac cagcaagtcc ttgtcggcgt tgagttaatt
     6601 acccatttct ccagtgaaga tggtacggtt cgtatcccga cgagtcgggt tatagcgcaa
     6661 cttaccgagg agcaacattg gcaattattt tcagagcagt tggaattact aaaatcatgc
     6721 caacattttt ttattcaaca taaactcttt gcctggctta atttaacgcc acaagtggct
     6781 acattattat tagagaggga taattacgca ggcgaactat taaaatatcc gtttattgaa
     6841 ctgttgatta atgagaatta cccgcatctc aatgaaggaa aagataatcg cggtctttta
     6901 tccttatcgc aagtgtatcc ccttgtgtta ggtaatttgg gagcaggcaa tagcacaatg
     6961 aaagcggttt ttgatggtct tttcacccgc gtcatgctgg ataaaagttt tattcagcaa
     7021 caaatcacgc accgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct
     7081 tgttgcaatt gcatcattgc gggaggcatt gatacggcag aaatattggc acagattacc
     7141 cccttcgatt tccatgctct ccagggatgc ctgtggcctg ccgttcccat aaatcagata
     7201 acgacgttgg ttcagcgata acccttctgt ttgctggtgt ttaagacgag agtaaccgtc
     7261 tacactatca aacaggagga tctatgaccc tgtcttttgt tacccgctgg cgcgatgaat
     7321 tgccagaaac ctatacagca ctttccccta cgcctttaaa taatgcccgg ctgatttggc
     7381 ataataccga actggctaac acgctgagta ttccatcgtc gctgtttaaa aatggcgcag
     7441 gtgtctgggg cggcgaagcc ctactgcctg gtatgtcacc actggcgcag gtttacagtg
     7501 gtcatcagtt cggcgtctgg gcgggccaac tgggtgatgg gcgcggcatt ttacttggcg
     7561 aacaactgct tgctgatggc actacaatgg actggcatct gaaaggtgct ggcctgacgc
     7621 cttattcgcg aatgggtgat ggacgggcgg ttttacgttc gacgatacga gaaagtctcg
     7681 ccagtgaggc gatgcattat ctgggcattc cgacgacccg cgcgttaagt atcgtcacca
     7741 gcgattcgcc agtgtatcgg gaaacggcgg agccaggcgc gatgctgatg cgtgtggcac
     7801 caagtcatct gcgctttggt catttcgaac atttttacta ccgccgcgag tcggaaaagg
     7861 ttcgtcagtt agctgacttt gctattcgtc attactggtc acatcttgca gatgatgagg
     7921 acaaataccg tctctggttt agcgatgttg tcgcacgtac cgcgtcgtta attgcccaat
     7981 ggcagacggt cggctttgct catggggtga tgaataccga caacatgtcg ctgctggggc
     8041 tgacgcttga ttacgggccg tttggttttc ttgatgatta cgaacccggt tttatttgta
     8101 atcactcgga tcatcaaggg cgttacagct ttgataatca acctgccgtc gcgttgtgga
     8161 atttacagcg tctggcgcag acattgtcgc catttgttgc cgtagatgcc ctgaatgagg
     8221 ccctggacag ctatcagcag gttttgttga cgcattatgg agaacggatg cggcagaaac
     8281 tgggcttcat gacggagcaa aaagaggata acgcgctact gaatgaatta ttcagtctga
     8341 tggcgcgaga gcgcagcgat tatacccgca cattccgcat gctgagtctg accgagcagc
     8401 acagcgcggc gtcaccgcta cgtgatgagt ttattgatcg tgcggcattt gatgactggt
     8461 ttgcccgtta tcgggggcgt ttgcaacaag acgaggttag cgatagtgag cgtcagcaac
     8521 tgatgcaaag cgttaacccc gctctggtat tgcgcaactg gttggcgcaa cgggcgattg
     8581 aagcggcaga aaagggtgat atgacggaat tgcaccgcct gcatgaggcg ttgcgaaatc
     8641 ctttcagcga cagagatgat gactatgtca gtcgtccacc tgactggggt aaacggctgg
     8701 aagtcagctg ctcgagttaa aggctacttg gtcaacagca gcttgccagc ctgagtttta
     8761 cggagcagat attcttgccc gtcatgatcg ataatcagtt tgccatccgg acctaacagt
     8821 gtctggctgg aaattcgccg aataggctgc ggggaagcgg tcttgtgatt ggcatccgtt
     8881 tcaggcgtga gttttctgct atccgtataa cgcataaaaa ttaataataa ttgatataac
     8941 aatgattctt attatcaata ttatggctga ccagagcaag cagtaatttg ttgagaagac
     9001 aacaagttgc gcagaaatgc ccattccgtc aggaatgggc acgcattcag aagcgggtat
     9061 ctaccgcaga ggcgagtttt tcgaccaggc gttcggtatc ctcccagccc agacacgggt
     9121 cggtaatgga ttgaccgtaa gtgagcggct gactgccgac gattttttgc gttccttcgc
     9181 gcaggaaact ttccgccata attccagcaa tcgccgtaga gccattgcgg atttgctgac
     9241 aaatatcctc acaaacttct aactggcgac ggtgctgctt ctggcagtta ccgtggctga
     9301 aatccaccac cagatgttca ggtaaatcaa actcgtgcag cgtatcgcag gctgcggcga
     9361 tatcatcggc atgataattc ggttttttgc cgccacgcat aataatgtgg ccatacgggt
     9421 tgccgctggt ctgatagatg gtcatctgac catttttgtc tggcgagagg aacatatggc
     9481 tggcgcgggc tgcgcggata gcatccacag caatccgcgt attgccatcg gtaccatttt
     9541 taaaacctac cggacaggag agtgccgaag ccatttcgcg gtggatctga ctttcggtag
     9601 tacgtgcgcc aatcgcgccc caactgatta aatcagcaat aaactgaccg gtcaccatat
     9661 cgaggaactc ggtcgcggtt gggacgccca gctcatttac ctgtaaaagt aatttgcgcg
     9721 ccagctccag accgtgattt acccgatagc tgccgtttaa atctggatcg gagattagtc
     9781 ctttccagcc gacaacagtt cgtggttttt caaaataggt gcgcattacg atttccagcc
     9841 gtgactggta ctggttgcgc agcgactgca gacgggtggc gtactccatt gcagcggtga
     9901 gatcgtggat cgagcagggg ccaataatga ccaacagtcg cttatcttca ccattcagta
     9961 ttttttcaat tctgcggcgg gagtcggtga catgggtggc gacgccaggc gttacgggat
    10021 accgtagcgc gagttcggcg ggcgttacca ggctctcaat acgcgcagta cggagttcgt
    10081 cagttctgtt cattacaagt ctcagaaatt tgttgcttct acggcaggac tgccgggaag
    10141 tgatgcgcat cacgataaac caatcgctgc tgatttcaag tgcgaggtgt gtcacacctc
    10201 gccgggtggt tagcatgata acaaaaaaat aagctaatgc actagttctc tagtacattc
    10261 ggcgactaag gcccatgata tcgaggatct tggtggcaat ctcttctacc gaataattgg
    10321 tactgttaat ccacgggatc tgatttttac ggtacaaggc ttccacttcc gcgacttcca
    10381 tcctgcactg acgaagcgag gcatagcgac tgttctcccg acgttcctcg cgaatcgccg
    10441 ccagacgttc cgggtcgata gtcaggccga acaatttatg ctgaagcggt ttgagcgacg
    10501 cgggtagcac cagattatcc atatcgtcgg caataaaggg gtagtttgcc gcgcggatac
    10561 caaactgcat tgccagatac agactggtgg gggttttacc acagcgagaa acaccgagca
    10621 ggatcacctg agcctggtcc agattgcgca acgaaatgcc gtcatcgtgg gcgagggtgt
    10681 aatcaatcgc cgcaatgcgc gcatcatatt tattgagatt attagggtta aggccatggg
    10741 tacgatgagc aatcggcgtt ggatccagtt tcatctcttg ttgtagcggg gcaaccagcg
    10801 cctgaacgat atcctggcaa aagccttcac tttgcaagat gatggcgcga atctccggca
    10861 acacgatgga gtagaagacc agcgggcgca cgcctgtctg gtgataaatt gcgtcaatct
    10921 gatccttcac tgcccgtgca cggctctcat tttcgacaaa cggcagcgtg atgctgctga
    10981 tagtgacggg aaattgtgac attactgcgt gtcctaatac ctccgcagtt attgccgtac
    11041 catcagaaat ataaaaaacg tggcgatcaa cagcattatc cattttgttc ttcccgtgat
    11101 gcagacataa ttctatgaaa gcataaatta aaaacgcaca gaagcgtaga acgttatgtc
    11161 tggtttataa aatgaacctt caattttatt ttttatgaaa acagcatttc atttttatgg
    11221 tttcgtttat accgatggtt tatgtggaaa ttgtcgaaga gagcagattt gcgcaacgct
    11281 gggatcagtc ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat
    11341 ccggttaaat atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca
    11401 caaaaggatt gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa
    11461 ctcggcatga atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt
    11521 actaatcttt ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg
    11581 tttaaccagt ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa
    11641 acggatattg acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt
    11701 atcgacactc ccttccagcc tgagctggaa aacgccatcc gcgaagccta tgcacagctt
    11761 tccgccgatg acgaaaacgc ctcttttgcg gtgcgctcct ccgccaccgc agaagatatg
    11821 ccggacgctt cttttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc
    11881 gttctcgtgg cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat
    11941 cgtgtgcacc agggttacga tcaccgtggt gtggcgctct ccgccggtgt tcaacggatg
    12001 gtgcgctctg acctcgcatc atctggcgtg atgttctcca ttgataccga atccggcttt
    12061 gaccaggtgg tgtttatcac ttccgcatgg ggccttggtg agatggtcgt gcagggtgcg
    12121 gttaacccgg atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc
    12181 gtgcgccgca ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac
    12241 ggcaagcagg ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc
    12301 aacgaagaag tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc
    12361 ccgatggata ttgagtgggc gaaagatggc cacaccggta aactgttcat tgtgcaggcg
    12421 cgtccggaaa ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag
    12481 ggtaagatta tcgccgaagg ccgtgctatc ggtcatcgca tcggtgcggg tccggtgaaa
    12541 gtcatccatg acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac
    12601 atgaccgacc cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt
    12661 ggcggtcgta cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg
    12721 ggctgtggag atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc
    12781 gaaggtgata ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc
    12841 gtagaaacga tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt
    12901 gctttcgact tcgcctgcct accgaacgaa ggcgtgggcc ttgcgcgtct ggaatttatc
    12961 atcaaccgta tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg
    13021 cagttgcaaa acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac
    13081 gttggtcgtc tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc
    13141 attgtccgtc tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt
    13201 tacgagccag atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc
    13261 gacagcttcc gcgactgttt cgcgctggag tgtgaagcag tgaaacgtgt gcgcaacgac
    13321 atgggactga ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa
    13381 gcggtggttg aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc
    13441 atcatgatgt gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc
    13501 gacggcttct caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac
    13561 tccggcgtgg tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg
    13621 atggctatcc gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg
    13681 tccgaccacg aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg
    13741 aacccggaca ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa
    13801 tccccggcgg cgtttagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc
    13861 tcttcacaga catcctgcgt taaacgccgc ataatatctt ttcttaacaa aaacttttgt
    13921 attttacctg aggtagttcg cggtagtttt tcgattacca cgatatgttc aggatattta
    13981 tattttgcga cccgtttacg gctaaaaaaa gccactacct cttccagcga taatgaatga
    14041 tgcggcgctt tcagcacgac ataagcgcat gatcgttcac ctaaacgttc atcggacatt
    14101 gcaaccacac aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg
    14161 ctgctaatat tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata
    14221 tagccagcct catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagg
    14281 gcacgggcgg ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgag
    14341 gcttcttcac cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc
    14401 tctacacctg cggcagcgta accatcggtg tgcataaagc gcgacaaagg atcatcgaga
    14461 ttcaccaccg catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgccg
    14521 cgctgctggc attcacgcgc cacttttttg gggattgtgg ttccgccgca aagaaagaaa
    14581 cgcagcgctg aaaggtccgc gggttgtttc tctagtacat tcaaaagatc atagacaaac
    14641 ggcgttgcgc cgagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca
    14701 ggagtgaaaa tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca
    14761 tgcagaaagc ccgttgcgtg accaagtggc gcaggcatca taaagacatc ctgccaggtc
    14821 agattcagtc gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc
    14881 acgccctttg gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg
    14941 tgggtcgtta tcgccgtggt cagtgaggta ttgtcggcga taatctgact taatgagagg
    15001 gaagaggtgg cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga
    15061 ttttgcagcg gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag
    15121 aacatttttg cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc
    15181 aacagcggca cggaaactgc accgattttc aggcaggcaa gatagataac ggtaaattca
    15241 caccagccag gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc
    15301 cagtttgcca gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga
    15361 ttatcgacca cggcaatttt gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc
    15421 agcgaagcat cgccccataa cccttgctga cgatacgccg cacgacgttg ttcgttaaac
    15481 gttaatgtca ctttcatgtt ggactctcga aacagaacat caggcccgag atgcgggcct
    15541 gtgggatgca tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc
    15601 actgttcgag aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg
    15661 cggaatcaaa atgaatgttt cctgcgtcat cctgcttata aagtccggca gggcaggctt
    15721 tcattaattt atggaattca ttgatatcgg gattttccgc cagaatgata tgcgggtggc
    15781 cctcatcaac atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct
    15841 ggctcatagc gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt
    15901 cttcgcatgt cccatgatca tttttcgtac cggctggttt ggtttgccgt cgatggtgaa
    15961 catctcgttc atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc
    16021 catcagcgcc gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag
    16081 ctcacgtttg tattgcgcca gactgctggc ggagaaatcc gcgcgttctt tggcggcgat
    16141 caccgttgtg gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa
    16201 acccaaattc aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag
    16261 ttgcggcacc attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt
    16321 gccgccgcta atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg
    16381 cacgcttttt tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga
    16441 atccttgttg gtatagagaa atcccccgcc catcaggccg tcagaagggg aaccggcaaa
    16501 cagccaggcg gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt
    16561 gaggccaata acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat
    16621 tcccagcgag cggccaagca tcgagttaac gccatcagcc agaatcacca cattcgcttc
    16681 gagaatatca tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc
    16741 gacgcgaacg cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg
    16801 gtccagacga ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gctgctcgcg
    16861 gtgaaaatcg agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac
    16921 cttgcgttct accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc
    16981 ataaagacgc ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag
    17041 gacatccagc ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc
    17101 gaccacaatg gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt
    17161 acagcgggag atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga
    17221 cgactttgta gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt
    17281 tatctttatt gatggcgaca atcacttttg cgccgttgcc gccaaccata tgctggatct
    17341 gcccggagat ccccagcgtc aggtagagat cggatttcag caacacgccg gagacaccga
    17401 tataacgttc acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt
    17461 cagcattcag caccgccgcc agttcgtgga ccatttttag gtcatcctgc gccgccagtc
    17521 cacgaccgac gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg
    17581 cacggcgttc gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg
    17641 ggcactgatg agaggtatca ctggtgcacg gttcctgaac accgggtgca agggtaatga
    17701 tcgccagcgg gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt
    17761 cggcgcaaat gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat
    17821 tcagttgcac acttaaccgt gctgccagcg ctttaccacg tttcgtcgcg gccaacagca
    17881 acatagcggg gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc
    17941 gttgcagcgc gtcgttttgc gcaagaacat aaagacattt tggaccataa ggcataactg
    18001 cctgcgcctg gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc
    18061 cgccaaacag ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt
    18121 gactcatcgt ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc
    18181 tcggcaatgg cctccggcga atcgttatcg ataatgatgt gcttacgttc tgtttgcggc
    18241 ggtacgcgaa tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt
    18301 gcctgccact gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg
    18361 cgtggcacgt taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact
    18421 tcaacatcat cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca
    18481 ctcactgcat taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg
    18541 gcataaaggt cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttttcaatt
    18601 gctgccgcca gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc
    18661 accaaataca ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc
    18721 aacaatgagc cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt
    18781 gcagcttcaa tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc
    18841 agggtgtatt ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt
    18901 attattttca tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa
    18961 atggcctgaa aatagtgtgc gatagcggtg cggggttttc gacggagcaa gctgttttcc
    19021 ttgccaacaa tcttgcaaaa aaaatgcgat cgctaaaaca aaagtgcaat ttgcggcaat
    19081 atagttattc catgaccgta caatcttgca ttcagtgtaa ataatcttgt ttagctctcg
    19141 ctaaacgcgg ttgtataacc gcaatacacg ccgtatttta tccggcatat attgtttttg
    19201 cttgctcatg gaatagggag gttgtcatgt atcaacgctg ttttgataac gccagtgaaa
    19261 cgctgtttgt cgccggtaaa acgccacggc tttcacgttt tgcatttagc gatgatccaa
    19321 aatgggagtc cggacatcac gttcatgaca atgaaaccga gttgatttac gtcaagaaag
    19381 gggttgcaag gtttaccatc gattcttcgt tatatgtcgc acatgcggat gacattgtgg
    19441 tgatagaacg cggcaggctg catgcggtgg cctctgacgt taacgatccg gcaacgacgt
    19501 gtacctgtgc gctgtacggc tttcagtttc agggggctga ggaaaatcag ctactgcaac
    19561 cgcattcttg tccggtaatt gccgcggggc agggaaaaga agtcataaaa accttattta
    19621 atgagctaag tgtgattttg ccgcaaagta aaaatagcca aacatcttcg ttatgggatg
    19681 catttgccta tacgttagcc attctttact acgaaaattt taaaaatgct tatcgttcgg
    19741 agcaggggta tattaaaaaa gatgttctga taaaagatat tcttttctat ctgaataata
    19801 attatcgcga aaaaatcact ttagaacagt tatcgaaaaa atttcgtgcc agcgtcagtt
    19861 atatttgcca tgaatttacc aaagagtatc gtatttcccc tattaactat gttattcaac
    19921 ggcgtatgac ggaagcaaaa tggtcactca ctaatactga attatcacag gcagagatct
    19981 cctggcgtgt gggttatgaa aatgtcgatc actttgccaa actgtttttg cgccatgtcg
    20041 gttgttcgcc cagcgattac cgtagacaat ttaaaaactg ttttgcggaa caagaaatcc
    20101 tatctgaatt tcctcaaccg gtaagtcttg ttggataaat ctggcggcgc agtttgttaa
    20161 gaagctgcgc cgcctgcaga ttatttgttc tgatagtctt tcaggatctg ccgacctgct
    20221 acgtaaatca taatttcgtc tgtaccgccg ccgatacgtt cacaacggac atcacgccag
    20281 aagcgggaga cgcgcgcctc atcggtatag cccagaccgc ccatgatttg aatcgcatca
    20341 tcaatgactt ccattgcggt acgtgcacag tacagttttg ccagcgccgc gctggtgcgc
    20401 agtgactgat gctgatcggc ttgccatgcc actttcagca ccatgttgcg catgttgtcg
    20461 atcttaatcg ccatcagcgc cagtttttcc tggatcatct ggttatgacc aatgggctta
    20521 ccaaaagcga tacgttggtt ggcatagcgg gcggcatctt caaaggcgca ttcggcaaag
    20581 ccggtgctgc gcgcggcgtt gatcaggcgc tccatctcaa agttgtacat cacattgagg
    20641 aaacccattc cttcttcgcc caccatgtcg ctctcttcaa cctccacgtt gtcgagatag
    20701 acttcgcagg tgctgagcat atgccagccg attttatgca gcgggttaat cttaatgccg
    20761 ggcttactgg agtcgaccca ccacagggtg aaggcttttt tgggatcttt cggttgcgga
    20821 tcgcgcgcca gcaccagcat atacgggtac tctttcgcac cggtaataaa ggttttttgt
    20881 ccgttgatgt agaccttgcc gtttttacgc gtgtaagtgg tagtggcact gttgttatct
    20941 gagccagcac ctggttccgt caacgccagg gcataggcgg gatcacccgt ttccagggtg
    21001 ctctctgccg ttttacgtag ctgctctgca gaaccgaaac ggcgcatact gtgaatacat
    21061 tgaccgttgg taattaaaaa tgccggagca ccgcattttg acacttccat cagcgccagc
    21121 atttgggtga cgtaatccgc agggatacca ccaaattctt ccggcacgcc aagcatggaa
    21181 ataccgttat ccgccagcgc ccgcataaac tcacgcggat atgtcccgtt ttgatcgcag
    21241 gtgcggaaat actcttccgg aaagttagtc gtaatcagtt cgcgaatact ggccagcagc
    21301 agttcttgtt cttcagttaa agaaaaatcc atcattatac tcctgttaat gagccgcttc
    21361 aggcaggaca aaacccatcg ccgcatcgat aaataatctt tcgtccatca gtttgagttc
    21421 tggcgaaatc actggggtga aatccatttt gtcgagaata tctttttgta aatcgacgcc
    21481 aggggcgatt tcgattaaat gcaggccgtc ttctttcagc gtgaatactg cgcgctcagt
    21541 gatataacga acatccagcc ctcgctcgag agcgattttt ccgctgaaag taatttccgg
    21601 tagttcccga ataaatttct tcacccgtcc ttcctggacg atatttaatt tgccgtcggt
    21661 aatttctgtt tttaaactgc ccgcagttaa tgtgccgcag aaaatgattt tcttcgaagt
    21721 ggcactgata tcaataaatc caccggtgcc catgatttta ccattgaatt tatgcacgcc
    21781 gacgttaccg tgctggtcga cttcagcaaa actcaaataa caaacatcca gaccgccacc
    21841 gtgataaaaa tcaaactggg acgtcatatc cagaatggca cgggtattca cgttcgcgcc
    21901 aaaggcgatc ccctgtgaag taattccgcc aatcggacct gtttctaccg tcagaataaa
    21961 gtcatcagca caaccttctt ctcgggcgac caggccaatg ccgtcagcaa taccgacgcc
    22021 gacattcccc accgcgcctt tacgcatttc gaataatgcg cgccgcgcaa ctaatttacg
    22081 ttggtttagg ggcaggctaa gtttggtact gtcatcaagg gtgaagtcac cagaaataaa
    22141 gcggttaacc ggtgcaccgc catacagttg ggtttgatcc ggatcgacca ccacaatatc
    22201 caccagataa cccggaatac ggacagattt aggatgcagc gtggctttct taaccatttt
    22261 ctgcacctgc atcatcacaa taccgccgtt attgtgcacc gcctgggcaa taaccaatgc
    22321 gtcgagatac atcacctcat cttcaaaagt ggcgtagcct tcactgtcgc aggtggtagc
    22381 gcgaatgaag gcaatatctg gcgcaatcgc tttgtaatag agatattctt tgttatcaaa
    22441 ctcgaccagt ttaatcaggt cttctttagt gacttcattc agtttgccgc cttgctggcg
    22501 tggatcgaca aatgtcccga tgccaatatc actaataata ccaggctggt gggctgcggc
    22561 ggcgcgtaag gtttgtgtaa gtacaccttg tgggtagtta taagcaataa ttttattttg
    22621 ttctgcgagt tcagaaatac gcggcgattg tccccagtga ccacataatg cccatttcac
    22681 cagaccttct tgcgccagag gactaatacc acggtcggcg cgatcgccaa gccctgttgg
    22741 actaataatc gataaattac gtggtgtttg agtctgttta tatttatcag caagagcagt
    22801 aattaacgtg gtggcttcca gaataccgcc gccagcgcct aacacacaaa gtgttgcttc
    22861 gtcgggaata taattcaccg cttcctgtgc cgacaggacc ggcactcgtc cattaatacg
    22921 aggtggtttt acaggtttca ttgttaattc ctgattcaga tgtgattttc cctacgccaa
    22981 atggagaatt agcgcacaga gactcacgaa atatttttat taaatacgat tgaatatatt
    23041 ttttagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc tggtgtaaaa
    23101 tagttaatac cgtgcgcaaa tcatttaccg agatttgccc tggcgcagac gcttttttta
    23161 ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa attacgccag
    23221 tttttgccat cgacatcgtg ataattggac gatcggcata ctgctcctgc atctccaggg
    23281 tcgcggcaag caacgtcagc acatcgctgg tactttgcgg catcagcgca atcttaggaa
    23341 tatcggcgtc gaaggattgc attttgcgca gacgggcaat gatttcttcg gcttccggcg
    23401 ttttatggaa gtcatggttg gacatgacta ctttcacatc atgcgcgtgg gcgtaggcga
    23461 cggtttcttt aacctgatca tcaccggtaa ataactccag atcgatcata tcaaccaggc
    23521 cgctgtcgat ggctgcacga ttgagtgcaa tataagcctc ggtggaaatc gcctgctcgc
    23581 cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg gtctcacgga
    23641 gaatttttgc tgccgccatg acagactcca cattggagag gtcggcatag tggtccacac
    23701 gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat ttcacgctgg
    23761 cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgtacca atgacgagat
    23821 cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc tgtcaggcac
    23881 cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc cataatgtga
    23941 actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg caatcagctt
    24001 gttgcgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca gtgaccagaa
    24061 gtcccggatg taacagactg atatcattaa ccaatgattc attctcaagg ggtttcatac
    24121 ccacttttgt gccattggtt aaaatgtcgg cggaagccag ggcttcagca aaggcttgct
    24181 gatcggcgag atcggtgacc gtgacgacac aatcggtgtt ttcattaacc cgctgcgcga
    24241 aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt tcttttaaac
    24301 cttcaattgc cccctgcgcg ccaattgccg ttgaggcacc accggcccct aacagcacca
    24361 tcgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt
    24421 cggtgttata gccacgcaga tagccatcat cattaacgat ggtgttgatg gcccccacca
    24481 gtttggcagc tggtgttaat tcatcaacat attcacacgc cagttgtttg ttcggcatcg
    24541 atacaccagt tccgcgcatt ttgagggctt ttaatccttc aattgctcca ggaaagctat
    24601 cgttatccac ttcgaaggcc atataggtaa atggcaatcc cgctttttct aaggctttat
    24661 tctgcatttc gggcgataaa ctgtggcgga taggataggc catcaaccca atcaattcgt
    24721 attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg tgtttaaccg
    24781 ggtgcaaaat ttacgctcgc caaaccgcac atcattttca ggaataatga aaacgcggta
    24841 atagcggata aaaacaataa ttgcggtaat cagggccagc agcgcgaaag taaaatcgag
    24901 aataatgata tattgcaggc cgatgttcga cagataaccg gtaatcagtg gaataacaaa
    24961 gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc ttttgggaaa
    25021 aaactctgac atgaccgaaa cgccgagctg taaaatgccg ccagctgctg agaaaccgat
    25081 aacaaaggct ccggcattgc acaccagtgg agaagggtac aggtagataa tggctgctgt
    25141 tattgttgcc agtgcagagt taaatacatt agcccagatg ggccggacca tttttttcag
    25201 tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag agatggtttt
    25261 taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca tccacaccac
    25321 aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa caccttccag
    25381 ccagactaac ggtttgctgt tcatttgcgg taattcatta gttacgctgg cgtccaccaa
    25441 ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaata gaatacccgg
    25501 aataatcagc ccatagccgt accagatatt attgagcaac atatagctca ccagcattgg
    25561 gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg aaccagaggc
    25621 tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg agttagcgat
    25681 acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg gaataccaaa
    25741 gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac caaacttatc
    25801 ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac cggaaattaa
    25861 gtaggcaatg cccgcgttgt cagtggaaaa cttttccgcc agagatgaca tattttgggc
    25921 aagcgtaata acactaatgc cgtgcaggaa gtagctgaag taaatacaaa gaacagccag
    25981 gataaatggc gtgctgaaag ccttattttg agacattttc acagctcctt gctgaaagat
    26041 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
    26101 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
    26161 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
    26221 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
    26281 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
    26341 cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
    26401 tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac
    26461 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
    26521 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
    26581 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
    26641 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
    26701 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
    26761 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
    26821 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
    26881 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
    26941 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
    27001 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
    27061 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
    27121 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
    27181 tggtctgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
    27241 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
    27301 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
    27361 gcgaggccat attcaggctc atcaaaagga cgcccatacc atgcaccagg taattaaaat
    27421 acaacccaag tgcggtaggg aaatagggat ttttcatagg ttctacttag caaaggtgct
    27481 ttaaaaagtg aagcgatgtt gtgtcagcat ccgttcatta ttgttgtaac aacgacaatt
    27541 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcagcta
    27601 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagactcgt tgaaatgcgg
    27661 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
    27721 tggcatgaag atgttttttt ctttgctgac gcatagccaa cagttgctcg actacacgat
    27781 caggaatggc acacttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
    27841 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
    27901 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
    27961 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
    28021 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
    28081 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
    28141 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
    28201 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctaccagctg aatggcggta
    28261 agcaggaaaa tatggggaag agcaagcaaa tatcaattag cggtggttaa caatcaggcg
    28321 gcagagccgc ctgatgagaa attacttatt cggtttctcg atttcgccaa gttcttcgag
    28381 aatttcttcc ggttggtccg tcggcggcgg gacttcttcc acccacgcgg caaacagacg
    28441 ccaggaaacg gctaacagaa ccggaccaat aaacagaccg atcatcccga aagcaatcaa
    28501 accaccaata acgccagaga gaatcaggat cagcggtaaa tcggcaccca tgcgaattaa
    28561 cattgggcgg atgacgttat ccagcgtgcc aaccacaccg ctccacacta acaataccgt
    28621 tccccaggtg gtatcgccag tccagtagag ccagataatc gccggaatca gtaccggcaa
    28681 cgggccaagc tggacaaggc aggagaggat cattaacacc gttagcaacg ttgcataagg
    28741 tacgccggat acggcgaggc cgataccgcc aagcactgcc tgtactaacg ccgtcaccac
    28801 cacacccagc gccaccgcgc ggatagcctg tgccgccagc agcacggcgg catctccgcg
    28861 aacgcctgcc agacgggttg caaaatggcg aatgccttgt gccacctgtt caccgcgcca
    28921 gtacagcagg gcactgaaga gaagcatcag cgcacaatgc accataaagc gcccgatatg
    28981 cgccgcctgc ccaacgaacc aggtggtggt ggtgccaata taagggcgga ctttcgccat
    29041 gatcgccgtc ccccccatat ccagcaagtt gtgccagcct gcatacagct tcgcgccaat
    29101 caccggaatg gtattaagcc acgccagatc gggtaacgtc atgtcaccgc tggaaatggc
    29161 tttaattagc gggccgctgc cgtcgacgat actgttaacc agcaaagcaa tagggatgat
    29221 aaacaccatc actaataaca gcgtcatcac cagaacggcg agagagcggc ggccaaacat
    29281 gatcttttgc aaacgtaaca ataccggcca ggtggcgata accaccgtac cggcccatgc
    29341 aaagccgaga ataaagggtt gaacaatcca cagacatgcc acaatcatga tggctaaaaa
    29401 cagcaccgaa agcagaattt gtgcgacatc cctgggctga cgaacattta ccataactac
    29461 ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg aacacgcgaa actcacctgc
    29521 ataatgatga gcaatttcag cggtttttaa caggccgatt ctgcccttaa ttctgatggg
    29581 cgtgttagaa aaaaatgtgg tacaacaact caggcaacac gcaaacgatt tactcgtcgt
    29641 atttcaagtt gcatgtgctg cggctacgtt cgttcacccc agtcacttac tattgtaagc
    29701 tcctggagtt cactcgcttg ccgccttcct gcaacacgaa atccatagag taaacaaaca
    29761 tcacctttca gtgcgctatt gcagcagcct gaaagaatag tgtatcgacc tacatcacag
    29821 accgcaggaa agggtcaata taatgattcc acagatttcc caggcacccg gcgtcgttca
    29881 actggtgctt aattttttgc aagagctgga gcaacaaggt tttaccggcg atacggcgac
    29941 aagttatgcc gatcgtctga caatgtcgac cgacaacagt atttaccaac ttctccccga
    30001 tgcggtggta tttccgcgtt caaccgcaga tgtggcgctg atcgcccgtc ttgccgcgca
    30061 ggaacgctat tcatcgctga tctttacccc ccgcggcggc ggcaccggca ctaacggtca
    30121 ggcgctcaac caggggatta ttgttgatat gtcccgccat atgaaccgca tcatcgaaat
    30181 taaccctgaa gagggctggg tgcgcgttga ggccggggtg ataaaagatc aactcaatca
    30241 gtacctgaaa ccgttccgct acttttttgc gccggaactt tcgaccagca accgggcaac
    30301 gctcggtggg atgatcaata ccgatgcatc cggtcaggga tcgctggtct atggcaaaac
    30361 gtcagatcac gtacttggcg tacgcgcggt gttgttgggg ggcgatattc tcgatacgca
    30421 acctttaccc gtcgaattgg cggaaacgct gggtaaatcc aataccacaa tcgggcgaat
    30481 ttataacacg gtttatcaac gttgccgtca gcaacgccag ttaattatcg acaacttccc
    30541 caaacttaac cgctttctta ccggttacga tctgcgtcat gtctttaacg atgagatgac
    30601 cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg acgctggcct ttattaccga
    30661 agcgcggctg gatattacac gcttgcctaa agtgcgccgt ctggtgaacg tcaaatatga
    30721 ctcttttgac tctgcgctgc gtaacgcgcc gtttatggtt gaggcgcggg cgctttcggt
    30781 agagacggtg gactcaaaag tgctgaatct ggcgcgggaa gatattgtct ggcattccgt
    30841 cagcgagttg attaccgatg tgcctgacca agagatgctc gggctgaaca ttgtggaatt
    30901 tgctggtgat gatgaggcgc tgattgatga gcgggtaaat gcactctgtg cgcggttgga
    30961 tgagctgatc gccagtcatc aagcaggtgt catcggctgg caggtgtgcc gtgagctggc
    31021 gggggttgaa cgtatctatg caatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa
    31081 aggtgccgct aagccaattc cgtttgctga ggatacctgc gtaccgccgg aacacctggc
    31141 ggattatatt gctgaatttc gcgcgctgct cgacagccac ggcttaagct acggtatgtt
    31201 cggtcacgtc gacgcaggtg tcttgcacgt ccgtccagcg ctggatatgt gcgatcctca
    31261 acaagagatt ttgatgaagc aaatctctga tgacgtggtg gcgctgactg cgaaatacgg
    31321 tggtttgttg tggggcgagc acggcaaagg ttttcgcgct gaatacagcc cggcgttttt
    31381 cggtgaggaa ctttttgcag aactgcgcaa agtgaaagcg gcatttgacc cgcataaccg
    31441 actcaaccca gggaagattt gcccgccaga aggtctcgat gcgccgatga tgaaagtgga
    31501 cgcggtgaag cgcggtacat tcgatcggca gatccccatt gcggtacgcc agcagtggcg
    31561 cggtgcgatg gagtgtaacg gcaacggttt atgcttcaac tttgatgccc gtagtccgat
    31621 gtgtccgtcg atgaagatca cccagaaccg gattcattca ccgaaagggc gcgcaacgct
    31681 ggtgcgtgaa tggctgcgtt tgttggcgga tcgcggcgtt gatccactca aactggaaca
    31741 agaactgcct gaatccggcg tcagtttgcg gacgttaatt gcccgcacgc gcaatagctg
    31801 gcatgcgaat aaaggcgaat atgacttctc acacgaagtc aaagaggcga tgtcgggctg
    31861 tctggcctgt aaagcgtgtt cgacccagtg ccccatcaaa attgatgtgc cggagtttcg
    31921 ctctcgtttt ctgcagctct atcacacccg ttatttacgc ccgctgcgcg accacctcgt
    31981 cgctacggtc gagagctacg cgccgctgat ggcacgcgcg ccgaaaacct ttaacttctt
    32041 cattaaccag ccgctggtgc gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc
    32101 gctgctgtcg gtcccctcgc tacaacaaca aatggtgggg catcgctcgg caaacatgac
    32161 gctggaacag cttgaatctc tcaatgcaga gcagaaagcg cgcacagtgt tggtggtgca
    32221 ggaccccttt accagctatt acgatgcgca agtggtggcg gattttgtcc gtctggtcga
    32281 aaaattaggt ttccagcctg tgttactgcc attttcgcca aatggcaaag cccagcatat
    32341 taaaggtttt cttaatcgtt ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg
    32401 tatggcgaag ctgggtatgc caatggtggg cgtcgatccg gcgctggtac tttgttatcg
    32461 cgatgaatat aaactggcgc tgggcgagga gcgtggcgag tttaacgtct tactggcgaa
    32521 tgaatggctg gcaagcgcac ttgagtcaca gccagtggct acagtcagcg gtgaatcatg
    32581 gtatttcttt ggtcactgta ccgaagttac cgccttgccg ggtgcgccag cacaatgggc
    32641 cgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc agcgtgggtt gctgcggcat
    32701 ggcagggact tacggacatg aagcgaaaaa ccatgaaaat tcgctcggga tctatgagtt
    32761 atcctggcat caggctatgc agcgactgcc gcgtaaccgc tgtctggcga ccggatattc
    32821 ctgccgtagc caggtaaaac gggttgaagg cacgggggta cgccatcctg tgcaggcttt
    32881 actggagatt attaaatgat atggaaacgg aaaatcaccc tggaagcact gaatgctatg
    32941 ggtgaaggaa acatggtggg gttcctggat attcgctttg aacatattgg tgatgacacc
    33001 cttgaagcga caatgccagt agactcgcgg acaaagcagc ctttcgggtt gctgcatgga
    33061 ggagcatccg tggtactggc cgaaagtatc ggttccgttg ccggttattt atgtaccgaa
    33121 ggtgagcaaa aagtggttgg tctggaaatc aatgctaacc acgtccgctc ggcacgagaa
    33181 gggcgggtgc gcggcgtatg caaaccgttg catctcggtt cgcgtcacca ggtctggcag
    33241 attgaaatct tcgatgagaa agggcgtttg tgctgttcgt cacgattgac gaccgccatt
    33301 ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt ttggtcatta tctgcacagt
    33361 aaacggtggt atactgtgca ggtcttgtct aaaagcgagg atgctatgtc tactcaactt
    33421 gatcccaccc agctggccat tgaattttta cgccgtgacc agagcaatct ttcacctgcc
    33481 caatatctca aacgtctgaa acaactagaa cttgaatttg ctgacctgct tactctctct
    33541 tctgctgaat taaaagaaga aatctacttt gcctggcgtc tgggcgtgca ttgatcctga
    33601 cttaatgccg caagttcgcc aaatcgacct ggattaacct ccgtccaggt tcgatgacct
    33661 tcttaatact gcttatctac cgcctgatat tgttcaaaaa taatactaat cgcagtttgt
    33721 gttaaaacgg cgggttagct ttatgagatt attcttatcg ccccttcaag agctaagcca
    33781 ctgagagtgc cggagataag cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag
    33841 acttaagggt tttcttattt ctgaacgctg cactttttta cctatctctt aaatagtcat
    33901 tttcaataca actttttctt tttcatcaca gccttaatgc tccttagata ataaccatta
    33961 tctaacaatg agatacctaa ttcttagcgt gcctgttaac ccacacatca gggtctatgc
    34021 ttattaaaag caggcaaaaa gcatgtttta cggtaaagcc cctgcgtttg ctgggttgaa
    34081 ctgataatca ttatcactaa catgctgtta tacgctgaaa gcgatgaagt gaggtaaatc
    34141 gatggacatg cattcaggaa cctttaaccc acaagatttc gcctggcaag gcttaacgct
    34201 gacacccgca gcggcgatac acatccgtga gctggtggca aagcagccgg gtatggtcgg
    34261 cgtgcgctta ggcgtgaagc aaacgggctg cgcgggcttt ggctatgtgc tcgacagtgt
    34321 tagcgagccg gacaaagacg atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc
    34381 gctgcaagcg atgccgttta ttgatggcac ggaagtcgat ttcgttcgtg aaggacttaa
    34441 tcagatattc aaatttcaca accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt
    34501 tggggtatag gcggtactat gtctcgtaat actgaagcaa ctgacgatgt caaaacctgg
    34561 accggcggcc cgctgaatta taaagaagga ttcttcaccc agttagccac cgatgagctg
    34621 gcaaagggga taaacgaaga ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggag
    34681 tggatgctgg agtttcgtct aaacgcctat cgcgcatggc tggagatgga agaaccgcac
    34741 tggttgaaag cgcactacga caagctgaat tatcaggatt acagctacta ctcagcacca
    34801 tcgtgcggta attgtgacga cacttgcgcg tctgaacctg gcgcggtgca gcaaactggc
    34861 gcgaacgcct ttttaagtaa agaggtggag gcggcgtttg agcagttggg cgttcccgtg
    34921 cgggaaggca aagaggtggc ggtggatgcc attttcgact cagtttcggt tgccactact
    34981 tatcgcgaaa aactggcgga gcagggaatt attttctgtt cctttggtga ggcgatccac
    35041 gatcacccgg aactggtgcg taaatatctc ggcaccgtgg tgccggggaa tgacaacttc
    35101 tttgccgcgc ttaatgcggc ggtagcctct gacggtacgt ttatttatgt gcctaaaggc
    35161 gtgcgctgcc cgatggaact ttccacctat tttcgcatta acgcagaaaa aaccgggcag
    35221 tttgagcgca ccattctggt ggccgacgaa gacagctacg tcagctacat tgaaggctgt
    35281 tccgctccgg tgcgtgacag ctatcagtta cacgcggcag tggtggaagt catcatccat
    35341 aaaaacgccg aggtgaaata ttccacggta caaaactggt ttcctggcga taacaacacc
    35401 ggcggtattc tcaacttcgt caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg
    35461 tcatggacgc aatcagaaac cgggtcagcg attacgtgga aatatcccag ctgcattttg
    35521 cgcggcgata actccattgg tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa
    35581 gcggataccg gcaccaagat gatccacatc ggtaaaaaca ccaaatcgac cattatctcg
    35641 aaagggatct ctgccggaca tagtcagaac agttatcgcg gcttagtgaa aatcatgccg
    35701 acggcaacca atgcgcgcaa tttcactcag tgcgactcaa tgctgattgg cgctaattgt
    35761 ggggcgcata ccttcccgta tgttgagtgt cgtaacaata gtgcgcaact ggaacacgag
    35821 gcaacgacat cacgtattgg tgaagatcaa ctgttttact gcctgcaacg cgggatcagc
    35881 gaagaagacg ccatctcgat gattgttaac ggtttctgca aagacgtgtt ctcggagctg
    35941 ccgttggaat ttgccgttga agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc
    36001 ggataaggaa taaacatgtt aagtattaaa gatttacacg tcagcgtgga agataaagct
    36061 atcctgcgcg gattaagcct cgacgttcat cccggcgaag ttcacgccat tatggggcca
    36121 aacggttcgg gcaaaagtac cttatcggca acgcttgccg ggcgagaaga ttatgaagtg
    36181 acgggcggca cggttgagtt caaaggcaaa gatttgcttg cgctgtcgcc ggaagatcgc
    36241 gcgggcgaag gcatctttat ggccttccag tatccggtgg agattccagg tgtcagtaac
    36301 cagtttttcc tgcaaacggc acttaatgcg gtgcgcagct atcgcggcca ggaaacgctc
    36361 gaccgctttg attttcagga tttgatggaa gagaaaatcg ctctcctgaa gatgccggaa
    36421 gatttattaa cccgttcggt aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat
    36481 attttgcaaa tggcggtgct ggaaccggag ttatgcattc ttgatgagtc ggactccggg
    36541 ctggatattg acgcattaaa agtggtcgcc gatggcgtga actcgctgcg tgatggcaag
    36601 cgctcattca tcattgttac gcactaccaa cgcattctcg actacatcaa gcctgattac
    36661 gttcatgtgc tatatcaggg acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa
    36721 ctggaggagc agggttatgg ctggcttacc gaacagcagt aacgcgctgc aacagtggca
    36781 tcacttgttt gaagctgaag ggacaaaacg ctccccgcaa gcacagcagc atttacaaca
    36841 attgctgcgt accggactgc cgacacgtaa acatgaaaac tggaaatata cgccgctgga
    36901 agggctgatc aatagccagt ttgtcagcat tgcgggagag atatccccac agcagcgtga
    36961 tgccttagcg ttaacgttag actccgtgcg gctggtgttt gtcgatgggc gttacgtgcc
    37021 cgcactgagc gatgcaactg aaggcagcgg atatgaagtg agcattaacg acgaccgtca
    37081 gggtttaccc gacgctattc aggcggaagt gtttctgcat ttgacggaaa gcctggcaca
    37141 aagcgtgacg catatcgccg tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat
    37201 gcatatcacc cagggcgtgg caggtgaaga ggtgaacact gcccattacc gacatcatct
    37261 ggatctggcg gaaggtgccg aagcaacggt gatcgaacat tttgtcagcc tgaatgatgc
    37321 tcgtcatttt accggggcac ggttcactat caacgtcgca gcgaatgccc acttgcagca
    37381 tatcaagctg gcgtttgaaa actcgctcag tcaccacttt gctcataacg atttgttgct
    37441 ggctgaggat gccaccgcat ttagccacag tttcctgctg ggtggcgcag tgttacgaca
    37501 caacaccagt acgcaactca atggcgaaaa cagcacgctg cggatcaata gcctggcgat
    37561 gccggtgaaa aacgaggtgt gtgatacccg tacctggctg gaacacaata aaggtttttg
    37621 taacagccga cagttgcaca aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg
    37681 tttgatcaac gtcgcgcagc acgccatcaa aacggatggt cagatgacca acaacaatct
    37741 gctgatgggc aaactggcgg aagtggatac gaaaccgcag ctggaaatct atgcagatga
    37801 tgtgaaatgc agccacggcg cgacggtggg gcgtattgat gatgaacaga tattctatct
    37861 gcgctcgcgc gggatcaatc agcaggatgc ccagcagatg atcatttacg ccttcgctgc
    37921 cgaactgacg gaagcactgc gtgatgaggg gcttaaacag caggtgctgg cccgaatcgg
    37981 tcaacggctg ccaggaggtg caagatgatt ttttccgtcg acaaagtgcg ggccgacttt
    38041 ccggtgcttt cgcgtgaggt aaacggtttg ccgctggctt atctcgacag cgccgccagt
    38101 gcgcagaaac cgagccaggt gattgacgcc gaggccgagt tttatcgtca tggctacgcg
    38161 gcggtgcatc gtggtattca taccttaagc gcccaggcga ccgagaaaat ggagaacgtg
    38221 cgcaagcggg catcgctgtt tattaatgcc cgttcggcgg aagagctggt gttcgtccgc
    38281 ggcacgacgg aagggatcaa tctggtcgcc aatagctggg gcaacagcaa cgtgcgggcg
    38341 ggcgataaca tcatcatcag tcagatggag caccacgcta acattgttcc ctggcagatg
    38401 ctttgcgcac gcgttggcgc agagctgcgt gtgatcccgc tcaatcccga tggtacgttg
    38461 caactggaga cgctgcctac gctgtttgat gagaaaactc gcctgctggc aattactcat
    38521 gtctccaacg tgcttggcac agaaaatcca ctggcggaaa tgatcacgct tgcgcaccag
    38581 catggcgcaa aagtgctggt ggatggcgct caggcggtga tgcatcatcc ggtggatgtt
    38641 caggcgctgg attgcgactt ttacgtgttc tccgggcata aactgtatgg ccccaccgga
    38701 attggcattc tttatgtgaa agaagccttg ttgcaggaga tgccgccgtg ggaagggggc
    38761 ggttctatga tcgccaccgt cagcctgagt gaaggcacta cctggaccaa agcaccatgg
    38821 cggtttgaag ccggtacacc caataccggg ggcatcattg gtcttggcgc ggcgctggag
    38881 tatgtttcgg cgctggggct taataacata gccgagtatg aacagaatct gatgcattat
    38941 gcgctatcac agctggaatc tgtaccggat ctcactctct atggcccaca aaacaggctt
    39001 ggcgttattg cttttaatct cggtaaacac cacgcctatg atgttggcag ttttctcgat
    39061 aattacggca ttgctgtgcg taccggacat cactgcgcaa tgccattgat ggcctattac
    39121 aacgtccctg cgatgtgtcg ggcgtcgctg gccatgtata acacccatga agaagtggat
    39181 cgtctggtga ccggcctgca acgtattcac cgtttgctgg gataacaggg aggcactatg
    39241 gctttattgc cggataaaga aaagttgctg cgtaattttt tacgctgcgc caactgggaa
    39301 gagaaatatc tctacattat tgagctgggc cagcgtctgc cagaattacg cgacgaagac
    39361 agaagtccac aaaatagcat tcagggctgt cagagtcagg tgtggattgt catgcgccag
    39421 aatgcccagg gaattattga attacagggc gacagcgatg cggcgattgt gaaagggctt
    39481 attgcggtcg tctttattct ctacgatcag atgacgccgc aggatattgt caatttcgat
    39541 gtgcgtccgt ggtttgaaaa aatggcgctc acccaacatc tcaccccatc tcgttcacaa
    39601 ggtctggaag cgatgattcg cgcaattcgc gccaaagccg ctgcacttag ctaaactaaa
    39661 aggacagctt tcatccggtt ggctcacatt gccggatggt caatgtttac actgacctgc
    39721 acaggagtgt tttggtttca ggtgaacata agcgccatcc gacgtttcag cgtgagtctc
    39781 cggcaaatac aggaggttta caatgaaacg cgcgtctttg cttacactca cgcttatcgg
    39841 cgcttttagc gccatccagg ctgcctgggc ggttgattat ccgctaccac caaccggaag
    39901 ccgactggtt gggcaaaatc aaacgtatac ggtgcaagaa ggggataaaa accttcaggc
    39961 tattgcccga cgttttgata ctgcggcaat gttgatcctt gaagccaata acactatcgc
    40021 cccggtgcca aaacctggta cgacgataac tattccttca caactgttat tacctgatgc
    40081 accgcgtcag gggattatcg ttaaccttgc agagctgcgc ctttattatt atccgccggg
    40141 agaaaatatt gtgcaggttt atccaatagg tattggattg caggggctgg aaacgccggt
    40201 gatggaaacg cgtgttgggc agaaaatccc taacccaacc tggacgccta cggcaggcat
    40261 tcgtcagcgt tcgctggagc gtggcattaa attaccgcca gtcgttcctg ccggaccaaa
    40321 taacccgcta ggacgttacg cactgcgcct cgcgcatggt aatggcgaat acctcattca
    40381 tggtaccagt gcgccggaca gcgtcggttt gcgcgtcagt tcagggtgta ttcgcatgaa
    40441 tgcaccggat attaaagcct tgttctccag cgtgcggacg ggaacgccgg tgaaagtgat
    40501 caacgaaccg gtgaaatatt ccgtggagcc taacgggatg cgttatgttg aagtacatcg
    40561 accactatcg gcagaagaac agcagaacgt tcagacaatg ccatacacac tgccagcagg
    40621 ctttacgcaa tttaaagaca ataaggctgt agatcagaag ttagtcgata aagcgttgta
    40681 tcgtcgggca gggtatccgg tttcggtgag cagtggagca actcccgcag ccagcaatgc
    40741 gccttcagta gagtcagcgc agaatggtga accagagcaa gggaatatgt tacgcgtgac
    40801 gcagtagcgg taaacggcag acaaaaaaaa tggcgcacaa tgtgcgccat ttttcacttc
    40861 acaggtacta ttacttgcgg tatttagtag ccatgttgtc cagacgctgg ttagcacgag
    40921 ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc gttcacgtcg ttgctcagct
    40981 ggtcaacttt agcgttcaga gtctgaacgt cagaagacag ctgatcgatt ttagcgttgc
    41041 tggagcaacc tgccagcaga gtagaaccca ggattaccgc gcccagtacc agtttagtag
    41101 ctttcattat taataccctc tagattgagt taatctccat gtagcgttac aagtattaca
    41161 caaagttttt tatgttgaga atattttttt gatgggaagg cacttatttt tgatcgttcg
    41221 ctcaaagaag catcgaacgc tgggttttag ataaaaaaat tgagagaaaa caggatgctt
    41281 ccatcggatt catcttagat aaagtgagat tatatagtga aatccgattt gattttttat
    41341 tgcttctggt tatcgattaa ataaaaaaag cgcccatcag ggcgcttcga tatacaaatt
    41401 aattcacaaa agcaatatta caggacgtga acagatgcgg tgttagtagt gccgctcggt
    41461 accagtgcac cagaaaccat aactacaacg tcacctttgt gtgccagacc gctctgcaga
    41521 gccagttctt tacccagacg gtagaaatca tcagtagaag tgatctcttt aacaagctgc
    41581 ggcacaacgc ctttgctcag taccaactga tgagccgttt tttcgttggt ggtcagtgcc
    41641 aggatggtgg catccgggaa gtatttacgt actgcgcgag cagatttacc gccctgagta
    41701 gcaaccacga tcagcggagc atccagtttt tcagcagttt caacggcacc acggcatacc
    41761 gcttcggtaa tgcgcagttt acggttgtca ttgttgaact cgagacggct gttcatcacg
    41821 cggtcggtac gttcgcagat ggtcgccatg atagaaaccg cttccagcgg gtatttacct
    41881 tttgcggatt caccagacag catcactgcg tcagtaccgt cgaggatggc gtttgcaacg
    41941 tcaccggctt ctgcgcgagt cgggcgtggg tttttgatca tggaatccag catctgggtc
    42001 gcagtgataa cgactttacg tgcacggata catttttcga tcatcatctt ctgggcgaag
    42061 ataacttctt ctaccgggat ttctacaccc aggtcgccac gcgcaaccat gatgccgtca
    42121 gaggcttcga ggatttcgtc gaagttgttg aggccttcct ggttttcgat tttggagatg
    42181 atgtggatgt tttcgccgcc gtgcgctttc aggtgctcac ggatttcgat aacgtcagaa
    42241 cgcttacgaa taaaggaagc agcaacaaag tctacgcctt gttcgcaacc aaagatcagg
    42301 tcctgtttgt ctttttcagc cagtgctggc agagcaatgg aaacgccagg caggttcaca
    42361 cctttgtttt cgcccaggtc accgttgttc agcactttac agataacttt gttaccttca
    42421 atggcggtaa cttccatacc gatcagacca tcgtcaacca gtacggtgtt gccaacagac
    42481 aggtcagtag tgaaaccttc atacgttacc gcaaccattt cgctgttgcc gataacagat
    42541 ttatcagtgg tgaaagtaaa ggtctgacca gctttcagag aaacgtcgtt accgccttcc
    42601 agtttcatgg tgcggatttc cggacctttg gtatcaagca ggatagcggc ggttttacca
    42661 gttttgctca tcacgttgcg cagattctga atgcgctgac cgtgttctgc atagtcacca
    42721 tgagagaagt tcagacgcat aacgttcatg ccagcgtcca gcattttagc taacatctct
    42781 tcagattcgg ttttcggtcc gatggtgcaa acaattttgg tctttttcat gacagtctta
    42841 gtctttaagt tgagaaggat gggagaaact tgctttctgg gcgcactgcc ggaaagcgaa
    42901 tccggtatta cgaaagtggt ggtgcctcgc tctaaggata ggtgacatcg aaaaagcgtg
    42961 cagaggaatg tgtgcttgtg tctcagccca acgtgagtgc ctgtttttga gtcgttgagt
    43021 tctacagtcc aaagtgctga aaccattcaa gagtcaattg gcgcgcatta tacgctgaaa
    43081 ttaatccaaa aggaaaacaa aaacagcgtt tcaaaaaata tacaacttgt tagacaataa
    43141 attgttatca aagatttaac gttccaggga aaaggttacg caaaaacttc actgtttgcg
    43201 caacgtctgg tgccgaagtt gtcagtgtga ggataagtga aagttcatcc ggtgtataaa
    43261 tatatcatcg ctcaattgct acagttaaaa taatttatcg cgttatttgt ctgattttgt
    43321 ttgatttttg tttaattggt tagattgata tatgctgata gttggcatga taaataggta
    43381 taaagaatgg gaaatcggac aaaagaggat gaactgtacc gagaaatgtg cagagttgtc
    43441 ggtaaagttg tgctggaaat gcgcgatctg gggcaggaac caaagcatat tgttatcgca
    43501 ggcgtattac gtacagcatt agccaataaa cgtattcagc gcagtgagct ggaaaagcag
    43561 gcgatggaaa ccgtcattaa cgcactggta aaatgaccat tcaggatgag gcggtaagct
    43621 tcatcctgat attcttgctt cagttgcggt ccagttaatc attctaaata acaactgctg
    43681 aaattatacg aatttcggta tgtattttaa tttatagcga taa
//
LOCUS       DBBGLQ010000034        41486 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.034, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000034 DBBGLQ010000000
VERSION     DBBGLQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.034"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..619)
                     /locus_tag="ACIYSB_002452"
     CDS             complement(<1..619)
                     /locus_tag="ACIYSB_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein E"
                     /protein_id="HGC5429488.1"
                     /translation="MPETIELPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYL
                     TLLRSRYPGKISKFDFTTLDVIATLNSNNTERAPGVLSDGGINTVQRKVLDYIRKAAQ
                     LDASDLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQ
                     FDPGVPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDS"
     gene            complement(629..1090)
                     /gene="pilP"
                     /locus_tag="ACIYSB_002453"
     CDS             complement(629..1090)
                     /gene="pilP"
                     /locus_tag="ACIYSB_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HGC5429489.1"
                     /translation="MHDKTKALLIAALLLPAMAVAGNENDASQNKQLQIEQIQEETAL
                     YEAMLAREKIRHELRTLEQGISPVSGSVTGHQPENAAGTSSGSQAFSSPRLKEIFGIQ
                     PQLSARVILPGGTETDVRAGQALPGTLFMVEQITPDSVILEYNGNRQTLRP"
     gene            complement(1080..2375)
                     /gene="pilO2"
                     /locus_tag="ACIYSB_002454"
     CDS             complement(1080..2375)
                     /gene="pilO2"
                     /locus_tag="ACIYSB_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HGC5429490.1"
                     /translation="MQKKIRPVSLISGKQAFLAGLDWKSIPPGYRNARRFARSQGAEL
                     YLSCHNHDGENGDEMMIALLSRQDADIPRNIRQCLSLAMLIRPLLKSGGYAIFIIGND
                     ENDDAELTCAFVSVINGILVNDVTGTPTEITEARNTFLMLNAEPENGWVRYEPDGFSP
                     DSRCIPLPLSNLISTRKHPAEARLLPVSRGPQLMTALLIITLLGASWYGWQRYEIWQA
                     EKAEREESARKAAEARITPPWTRQPESGEFIRQCSTTWNQTPLSAAGWRYTLAECSSD
                     GNSGNLRSSYTNTAGTTVTAFIRRITELTDTRPFIVMPEGNSGGVALPVTFRLPDNPV
                     PVDSLPETRDLQERLTTLAQSVQLQIDWQEVNNSFTDENGNIIQPPWKEYDLQIQTLL
                     PANQLAERFSEPSVRFLSVTRQLENGRFRYQFTGKYYAR"
     gene            complement(2399..4024)
                     /locus_tag="ACIYSB_002455"
     CDS             complement(2399..4024)
                     /locus_tag="ACIYSB_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HGC5429491.1"
                     /translation="MKKRQHMLLPLTAVALLLSGCGTAERISNTEKDATATLEKARRH
                     AGELRKSRPVVTETTRQWINPSPVSVSPAHQSLPGCPIIINTRRDISLRQIAQRITGS
                     CGIPVTITADVWQALATTGPTQQLTGAIPAPDPNGMVPLAAVGSGAQVIPPGGGAVLP
                     ELSATGLRQLLDTVASRLGISYRYDSDRRAITFYWLETRTFPVTYMDSQVTYNARVVS
                     GTASSSSGSSTGSNAGTLSGEASNTQTTTVDMKSTLYEDLKNAVGAMLTPGTGRMVLA
                     TGFLTVTDTPRVLDTVSRFVEARNREMRRQVVLNVEVLSVRKTRRDQAGIDWNAVFSD
                     GRLGLTLGGAFSSAADDIITSGVSIVDGKLTGSKAFLKALSSQGTVSVVTQNSAVTKN
                     MTPVPMQVASQQGYIEGVTTDTTANVGTSTSLNAATITTGFNMTLLPFIQPDARSLQL
                     LFSMNLSDKPTFEVFESGGSRAQVPTVDLKTINQTIDLKSGQTVILSGFRQSGSKSVR
                     QGVGSPAFFGLGGGTDSGDDDTILVVLITPHVI"
     gene            complement(4214..4690)
                     /gene="pilM"
                     /locus_tag="ACIYSB_002456"
     CDS             complement(4214..4690)
                     /gene="pilM"
                     /locus_tag="ACIYSB_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HGC5429492.1"
                     /translation="MCGARQRQTGRLTMGWSLSLVMALTVALTWFHNSSTHRVTVYSE
                     QAEMRERAWEMVRLMNGINDRLYTRPAERTGGGTLPVTATGFRPVYGTRHIIAGQRVF
                     VWQDDRPGLAGALADITHKTALAGRVKGRRLLNTTGQDTGISVPSVIPDSAFVYFN"
     gene            complement(4654..5379)
                     /locus_tag="ACIYSB_002457"
     CDS             complement(4654..5379)
                     /locus_tag="ACIYSB_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HGC5429493.1"
                     /translation="MTPLKPLLIVPLLAFSVPLFAAPSPSQVSAPSSSTVSSAPGALK
                     FIPRDGKGLSLKKAMQQLIPPGWTFDIAPDVRPPRTVSWKGNDQWNYVTERMLKKYQL
                     RAITDTEKKHVRIERNTLFSPSQATPGTTSLQEKTAEKSANMPQPAHARTTWTLARGT
                     LLRAGLEQWVAAVPCQAGGTWQLAWQNPGLNYSIDAPLHFTGEFRHALSELFRLYQDA
                     QTPLYAAINSTQCVVHVSDRPGD"
     gene            complement(5432..5650)
                     /locus_tag="ACIYSB_002458"
     CDS             complement(5432..5650)
                     /locus_tag="ACIYSB_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HGC5429494.1"
                     /translation="MTATTDTTPHKYRIEATILKPGGAPVSWVRFQNTPMTEPECVRL
                     FLKEKEAGKSFSVKVNVTAFSCSRVQPE"
     gene            6177..7685
                     /locus_tag="ACIYSB_002459"
     CDS             6177..7685
                     /locus_tag="ACIYSB_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGC5429495.1"
                     /translation="MSLNPTDEQSAIIHWQGEKLVVNAFAGTGKTSTLVQFAAACPES
                     RMLYLAYNRAIRDEAERRFPFNVECKTSHQLAYSRFGKHFRHRLVPGLSVTDVARKLN
                     TRYWTLARVAMTALNQFICSADVAPGMQHMPPPDEMKGMSPQDALRAVQILWHEMSNP
                     DGNFPVTHDTYLKLYQLSSPDLSRRWDTILFDEAQDANPVTSALVLGQRCRVVLVGDR
                     YQQIYRFRGASDALSHPALNGADRLWLTQSFRFGPAVAGVASQLLKRAGESREVTGRG
                     GDDEVLTAGEMRARVKAGKMTGHRAILSRTVAGVIGTALMAAMRGRKVYWVGGIEGYR
                     TEALEDLYWFQMDMPERMHSDRLRKDYRGFDEYRHIAKVTKDVEMNQSIRLLELCFPL
                     PKKLELLRKHTVTNEQEADITVSTAHRSKGLEWSLVVLDDDFQDITDPLISEQERRDE
                     TNLLYVAVTRARRTLVLNELMQQLTEAAGDGGAPGNAPVQGKEDDGVEGEDV"
     gene            7678..9207
                     /locus_tag="ACIYSB_002460"
     CDS             7678..9207
                     /locus_tag="ACIYSB_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HGC5429496.1"
                     /translation="MFKRFRRMLPGTKGTEPGVVAPVIPAGFYAPLTAEALLSPEHRE
                     LRLRQVWDNNPMPRDVYDRMCLEPLSRLLLNTQNVPATREGRWSRAGGFGDLTVLYTT
                     YAVRLARGYMFPPDATPEDQAAQAAVWHTVIFWSALFYHLPLLAHLEGELLSGRGWQP
                     GISVPDEPFRFRFRKTAPQGTEAQQLAALTAGTLLPDGATAWLVTAPGALQNLAGALW
                     HQHPGMALIRDVLQEAARQTESPLNTCAVTAPVTAEASADIRPADPVVTGSTDSVVAV
                     TEVQPAEEVKTESVQTETEGGGESAGASEVPSAQQELTVQTAPVVTADDEAVSAAPAD
                     QDTAMLLSLFSADESETAEPENSIKSSADRDGQSDVPAVQGEQTSVIYPEGEADTEEV
                     DFITWLKSGLSSGMIEINTPSARVHCIAGYVFLCVPDIFYLYQKMTGSRRDRRSVQAL
                     FERSRIHKIREGERFFSLHLYTVPDRTGSYKAVSGYLVKSRYLCGYNAVPGDSEYIAF
                     P"
     gene            9250..10350
                     /locus_tag="ACIYSB_002461"
     CDS             9250..10350
                     /locus_tag="ACIYSB_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5429497.1"
                     /translation="MKKDVEKITWEELLFEYFLEKNLKPDTEHSYRKVVRLFDKFLGG
                     DSWPGDVTPRDIQRWRRHMLKDQGRSVNTWNNRVSHLRPLLALGIKKKWLPQTENPLD
                     GTSVPGASKQKKTLTDKEMVRIYRTVQAFAEAEANKMVICDRRRNAMYPAWYWLIVLD
                     TLRFTGMRFNQLKHIRLKDIHEDEAVITLQLEGSKTRREWRVPIVESLRLSLKHLIEC
                     ARVNGASDNDYLFDVCLYSEHYSPGKYQYCAKRAHQSVKSFFRRLSRECGFFVSSHRF
                     RHTLATKLMAAPERNLHMVKSILGHRSVATTMEYIDFSVEVAAEALEAELGLSTDEAT
                     KRRIKEAKKRREASTGKKEKQESSGGELSDKE"
     gene            complement(10555..10630)
                     /locus_tag="ACIYSB_002462"
     tRNA            complement(10555..10630)
                     /locus_tag="ACIYSB_002462"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10595..10597),aa:Phe,seq:gaa)
     gene            complement(10736..11443)
                     /gene="yqgA"
                     /locus_tag="ACIYSB_002463"
                     /pseudo
     CDS             complement(10736..11443)
                     /gene="yqgA"
                     /locus_tag="ACIYSB_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
     gene            11596..11679
                     /gene="yqgH"
                     /locus_tag="ACIYSB_002464"
     CDS             11596..11679
                     /gene="yqgH"
                     /locus_tag="ACIYSB_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HGC5429498.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            11841..13976
                     /gene="speC"
                     /locus_tag="ACIYSB_002465"
     CDS             11841..13976
                     /gene="speC"
                     /locus_tag="ACIYSB_002465"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGC5429499.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPYR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPAAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLVE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGVKRLYGYVLK"
     gene            complement(14026..15282)
                     /gene="nupG"
                     /locus_tag="ACIYSB_002466"
     CDS             complement(14026..15282)
                     /gene="nupG"
                     /locus_tag="ACIYSB_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGC5429500.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(15484..16563)
                     /gene="mltC"
                     /locus_tag="ACIYSB_002467"
     CDS             complement(15484..16563)
                     /gene="mltC"
                     /locus_tag="ACIYSB_002467"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGC5429501.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(16628..16903)
                     /gene="yggX"
                     /locus_tag="ACIYSB_002468"
     CDS             complement(16628..16903)
                     /gene="yggX"
                     /locus_tag="ACIYSB_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGC5429502.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(16931..17983)
                     /gene="mutY"
                     /locus_tag="ACIYSB_002469"
     CDS             complement(16931..17983)
                     /gene="mutY"
                     /locus_tag="ACIYSB_002469"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGC5429503.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            18144..18863
                     /gene="trmB"
                     /locus_tag="ACIYSB_002470"
     CDS             18144..18863
                     /gene="trmB"
                     /locus_tag="ACIYSB_002470"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGC5429504.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            18863..19189
                     /gene="yggL"
                     /locus_tag="ACIYSB_002471"
     CDS             18863..19189
                     /gene="yggL"
                     /locus_tag="ACIYSB_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGC5429505.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            19373..20092
                     /gene="yggN"
                     /locus_tag="ACIYSB_002472"
     CDS             19373..20092
                     /gene="yggN"
                     /locus_tag="ACIYSB_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGC5429506.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            20268..21314
                     /gene="ansB"
                     /locus_tag="ACIYSB_002473"
     CDS             20268..21314
                     /gene="ansB"
                     /locus_tag="ACIYSB_002473"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGC5429507.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKTGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            21431..22438
                     /gene="yggM"
                     /locus_tag="ACIYSB_002474"
     CDS             21431..22438
                     /gene="yggM"
                     /locus_tag="ACIYSB_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGC5429508.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(22593..23729)
                     /gene="hemW"
                     /locus_tag="ACIYSB_002475"
     CDS             complement(22593..23729)
                     /gene="hemW"
                     /locus_tag="ACIYSB_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGC5429509.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEATDKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(23722..24315)
                     /gene="rdgB"
                     /locus_tag="ACIYSB_002476"
     CDS             complement(23722..24315)
                     /gene="rdgB"
                     /locus_tag="ACIYSB_002476"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HGC5429510.1"
                     /translation="MQKVVLATGNVGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTALPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(24323..24613)
                     /gene="yggU"
                     /locus_tag="ACIYSB_002477"
     CDS             complement(24323..24613)
                     /gene="yggU"
                     /locus_tag="ACIYSB_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGC5429511.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(24610..25176)
                     /gene="yggT"
                     /locus_tag="ACIYSB_002478"
     CDS             complement(24610..25176)
                     /gene="yggT"
                     /locus_tag="ACIYSB_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGC5429512.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(25194..25898)
                     /gene="yggS"
                     /locus_tag="ACIYSB_002479"
     CDS             complement(25194..25898)
                     /gene="yggS"
                     /locus_tag="ACIYSB_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGC5429513.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            25916..26896
                     /gene="yggR"
                     /locus_tag="ACIYSB_002480"
     CDS             25916..26896
                     /gene="yggR"
                     /locus_tag="ACIYSB_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGC5429514.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHRVGEGRL"
     gene            complement(27080..27496)
                     /gene="ruvX"
                     /locus_tag="ACIYSB_002481"
     CDS             complement(27080..27496)
                     /gene="ruvX"
                     /locus_tag="ACIYSB_002481"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGC5429515.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(27496..28059)
                     /gene="yqgE"
                     /locus_tag="ACIYSB_002482"
     CDS             complement(27496..28059)
                     /gene="yqgE"
                     /locus_tag="ACIYSB_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGC5429516.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(28168..29118)
                     /gene="gshB"
                     /locus_tag="ACIYSB_002483"
     CDS             complement(28168..29118)
                     /gene="gshB"
                     /locus_tag="ACIYSB_002483"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGC5429517.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(29131..29862)
                     /gene="rsmE"
                     /locus_tag="ACIYSB_002484"
     CDS             complement(29131..29862)
                     /gene="rsmE"
                     /locus_tag="ACIYSB_002484"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGC5429518.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(29942..30649)
                     /gene="endA"
                     /locus_tag="ACIYSB_002485"
     CDS             complement(29942..30649)
                     /gene="endA"
                     /locus_tag="ACIYSB_002485"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGC5429519.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCTKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(30744..31241)
                     /gene="yggI"
                     /locus_tag="ACIYSB_002486"
     CDS             complement(30744..31241)
                     /gene="yggI"
                     /locus_tag="ACIYSB_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGC5429520.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(31318..32712)
                     /gene="galP"
                     /locus_tag="ACIYSB_002487"
     CDS             complement(31318..32712)
                     /gene="galP"
                     /locus_tag="ACIYSB_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGC5429521.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(33136..34290)
                     /gene="metK"
                     /locus_tag="ACIYSB_002488"
     CDS             complement(33136..34290)
                     /gene="metK"
                     /locus_tag="ACIYSB_002488"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGC5429522.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            34346..34597
                     /gene="yqgD"
                     /locus_tag="ACIYSB_002489"
     CDS             34346..34597
                     /gene="yqgD"
                     /locus_tag="ACIYSB_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGC5429523.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTLFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            34708..34851
                     /gene="yqgG"
                     /locus_tag="ACIYSB_002490"
     CDS             34708..34851
                     /gene="yqgG"
                     /locus_tag="ACIYSB_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGC5429524.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            34945..35076
                     /gene="yqgB"
                     /locus_tag="ACIYSB_002491"
     CDS             34945..35076
                     /gene="yqgB"
                     /locus_tag="ACIYSB_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGC5429525.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            35085..37061
                     /gene="speA"
                     /locus_tag="ACIYSB_002492"
     CDS             35085..37061
                     /gene="speA"
                     /locus_tag="ACIYSB_002492"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGC5429526.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGIFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            37199..38119
                     /gene="speB"
                     /locus_tag="ACIYSB_002493"
     CDS             37199..38119
                     /gene="speB"
                     /locus_tag="ACIYSB_002493"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGC5429527.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(38325..39083)
                     /gene="loiP"
                     /locus_tag="ACIYSB_002494"
                     /gene_synonym="yggG"
     CDS             complement(38325..39083)
                     /gene="loiP"
                     /locus_tag="ACIYSB_002494"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGC5429528.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGNLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            39361..41352
                     /gene="tkt"
                     /locus_tag="ACIYSB_002495"
     CDS             39361..41352
                     /gene="tkt"
                     /locus_tag="ACIYSB_002495"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGC5429529.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
BASE COUNT         9694 a        10542 c        11449 g         9801 t
ORIGIN      
        1 gactgtcatc cttgatcaga cggagcacag catagaggcc gcccacgcag ggatagtggg
       61 agtagcgtgc accaaacaga ccggccagtt tcaggtactg ctctgaaaga cgcgcatcct
      121 gaggcacccc gggatcaaac tgtgtgccct tgatgacgtc ggtcatgccg gaataggtgg
      181 cccccagtaa ttcgaggcct tcttctttcc ggaggatatc gagcacttcc agttcgccgt
      241 gaacccgggc ttccacatag gtgaagtccg tgttgtctcg tcccggggtc atatgaaggt
      301 ctgaggcatc cagttgagcg gcttttctga tgtaatcaag aacttttctt tgtactgtgt
      361 tgatccctcc gtcggataaa acgcccggtg cacgttccgt attattgctg ttcagggttg
      421 cgataacgtc aagagtggta aaatcgaatt tgctgatttt tcccggatag cgggagcgta
      481 agagcgtcag gtaacgttgt acagaggtga tgtgcttctt cgcctcgacg actttcaccc
      541 tgatttttcc gtcagcgtca tgctgaattg cgacgaaatc acgaatatct tcaggaggca
      601 gttcgatagt ctctggcatg atggcacctc acgggcgtag cgtctgcctg ttgccgttgt
      661 attccagaat cacactgtcc ggtgtgattt gttccaccat gaacagtgta ccgggtaatg
      721 cctgtccggc cctgacatca gtttcagttc ctccggggag tatgacgcgt gctgaaagct
      781 gtggctgaat accaaagatt tctttcagac ggggggatga aaatgcctgt gaaccggagg
      841 aggtgccagc cgcattttcc ggctgatgtc ccgtgacaga accggaaacc ggggagatac
      901 cctgttccag tgtccgcaat tcatgacgaa ttttttctct tgccagcatc gcttcataca
      961 gggcggtttc ttcctgaatc tgctcaatct gcagttgttt gttctgtgac gcgtcatttt
     1021 cgttgccggc cactgccatt gccggtaaca gcagggctgc aatcagcagc gcttttgttt
     1081 tatcgtgcat aatatttacc tgtgaactga taacggaaac gaccattttc cagttgtctt
     1141 gtgacagaga gaaaacggac agatggctcg ctgaagcgct ctgccagctg gttagccggg
     1201 agtagcgtct gtatctgcag gtcatactct ttccacggtg gctggatgat gtttccgttt
     1261 tcatcagtga agctgttatt gacttcctgc cagtcaatct gcagttgaac tgactgtgcg
     1321 agcgtggtca gtcgttcctg caggtctcgg gtttccggaa gggaatcaac cgggacagga
     1381 ttatccggga gccggaatgt gaccggcagt gcaacgccgc cagagttccc ttcaggcata
     1441 acaataaacg gccgggtgtc tgtgagttca gtgatgcgcc ggatgaaggc tgtcactgtc
     1501 gtgcctgctg tattggtgta tgaagaccgc agattaccgc tgttaccgtc agaagaacat
     1561 tcagccaggg tataacgcca tccggcagca gacagtggcg tctggttcca tgttgtgctg
     1621 cattgccgga taaattctcc gctctcaggc tgcctcgtcc atggtggcgt tatacgtgct
     1681 tcagctgctt tccgggcgct ttcttcccgt tccgcttttt ccgcctgcca gatttcataa
     1741 cgttgccagc cgtaccacga tgcgccaagc agcgtgatga tgagtaaggc tgtcattaac
     1801 tgtgggcctc tggacacggg gagcagacgc gcttctgccg gatgctttct ggtgctgatc
     1861 aggtttgaca gcggtagcgg gatacaccga ctgtctggtg aaaaaccgtc aggttcgtag
     1921 cgtacccagc cgttttccgg ttccgcattg agcatcagga acgtgttgcg ggcttccgtg
     1981 atttcagtcg gggtaccggt aacgtcgtta accagaatgc cattaatcac cgatacaaac
     2041 gcacaggtga gttcagcgtc gtcgttctcg tcgttcccga tgatgaaaat ggcataccca
     2101 cctgatttga gtagcgggcg tattaacatt gccagtgaca ggcactggcg aatgttacgg
     2161 gggatatctg catcctgacg actgagcagg gcaatcatca tttcatcacc gttttcaccg
     2221 tcgtggttgt ggcaggaaag atacagttct gcgccctgtg accgggcaaa ccgccgggca
     2281 ttccggtaac cgggaggtat gctcttccag tcaaggcccg caaggaatgc ctgtttaccc
     2341 gatatcagtg aaaccggacg aatttttttc tgcattgacc cgttcaaggg aacctccgtc
     2401 agattacgtg cggtgtgatc agcacaacca gaatggtgtc atcgtcaccg ctgtcagtgc
     2461 cgccacccag cccgaagaat gccgggctgc cgacgccctg gcgcacggat ttgctgccgg
     2521 actgccggaa tccgctgaga atgacggtct gacctgattt caggtcgatg gtctggttga
     2581 tggttttcag gtccaccgtg ggtacctgtg cccggctgcc gccgctctcg aacacctcaa
     2641 aggtgggttt gtcggagagg ttcatgctga agagcagctg caggctgcgc gcatccggct
     2701 gaatgaacgg cagcagggtc atgttgaagc cggtggtgat ggtggcggcg ttcagggagg
     2761 tgctggtccc gacgttggcc gtggtgtcgg tggtgacacc ttcgatatag ccctgctggc
     2821 tggccacctg catgggcacc ggcgtcatgt ttttggtgac ggcggagttc tgtgtgacca
     2881 cgctgacggt gccctgactg gagagcgcct tcaggaaggc cttcgagccg gtgagcttac
     2941 cgtccacaat ggagacgcca ctggtgatga tgtcatcggc ggcactgctg aatgcccctc
     3001 ccagcgtgag gccgagacgt ccgtcgctga agacggcatt ccagtcaatg ccggcctggt
     3061 cacggcgggt ttttctgacg ctgaggactt ccacgttcag caccacctga cgtcgcatct
     3121 cgcggttacg tgcctccaca aaacggctga cggtgtcgag gacccgaggg gtgtcggtga
     3181 cggtgagaaa gccggtggca agcaccatcc ggccggtccc gggggtcagc atggcaccga
     3241 cggcattttt caggtcctca tagagggtgg acttcatgtc gaccgtggtg gtctgggtgt
     3301 tgctggcctc cccgctgagg gtgccggcgt tactgccggt actgctgccg gatgaggagg
     3361 aggcggtgcc gctgaccacc cgggcgttgt aggtcacctg agagtccatg taggtgaccg
     3421 ggaaggtgcg tgtctccagc cagtaaaagg tgatggcgcg ccggtcactg tcgtaacggt
     3481 acgagatacc gagccgggag gcgacggtat ccagcagctg ccgcagcccg gtggcgctga
     3541 gttccggcag gacggcaccg ccgcccgggg ggatgacctg tgcgccactg ccgacggcgg
     3601 ccagcggcac catgccgttc gggtccggtg cggggattgc gcctgtcagc tgctgtgtgg
     3661 ggccggtggt ggccagcgcc tgccagacgt cggcggtgat ggtcacgggg ataccgcagc
     3721 ttccggtgat gcgctgggct atctgccgca ggctgatatc ccggcgggtg ttgatgatga
     3781 tggggcagcc cggcaggctc tggtgtgcgg ggctgacgct gacaggggag gggttgatcc
     3841 actgtcgcgt ggtctccgtg accaccggcc gggatttacg gagttcgccc gcgtgccggc
     3901 gcgctttctc cagtgtggcg gtggcgtctt tttccgtgtt gctgatacgt tcggccgtac
     3961 cgcagcctga cagcagcagg gcgacggcag tcagtggcag gagcatgtgt tgtctttttt
     4021 tcatgcagaa atccttgtgt gaaacgtgaa aacagtgagc agagcaggga gggcagaagc
     4081 gcttcgggac tgaagtctgc gcgtgatggc gatatatccg cgggacatga ccgcggccgg
     4141 atatgcagac ttcagtccac aggctcctca cactgtgcgg gacagcgaag gttaacggtg
     4201 cagtgatgtc ccgctagttg aaataaacaa atgcgctgtc aggaatgacg gacggcacgc
     4261 tgataccggt gtcctgacct gtggtgttca gcagtcgccg ccccttcacc cggcctgcca
     4321 gcgcggtttt gtgggtgatg tctgccagcg cacctgcaag ccccgggcgg tcatcctgcc
     4381 agacaaagac ccgctgtccg gcgatgatat ggcgggtacc gtataccggg cggaaacccg
     4441 tggcggtgac ggggagcgtg ccaccccccg tgcgctcagc cggacgggtg tacagccggt
     4501 cgttaatgcc gttcatgagg cggaccatct cccacgcacg ttcacgcatc tccgcctgct
     4561 cgctgtacac ggtgacccgg tgggtactgc tgttgtgaaa ccaggtcagc gccacggtca
     4621 gggccatgac cagggagagc gaccagccca tggtcagtct cccggtctgt cgctgacgtg
     4681 caccacacac tgcgtgctgt tgatggctgc atacagcggc gtctgtgcgt cctgatagag
     4741 acggaagagt tcactgagcg cgtggcggaa ctcgccggtg aaatgcagcg gcgcatcgat
     4801 gctgtaattg agtccggggt tctgccaggc cagctgccag gtgccaccgg cctgacaggg
     4861 aacggcggcc acccactgct caagaccggc acgcagcagg gttccgcggg caagagtcca
     4921 ggtggtccgt gcatgcgccg gttgcggcat gtttgctgat ttttccgcgg ttttttcctg
     4981 cagactggtg gtgcccgggg tggcctgaga cggactgaac agggtgttcc gctcaatacg
     5041 tacatgcttc ttctccgtgt cggtgatggc gcggagctga tattttttca gcatgcgttc
     5101 cgtaacgtaa ttccactggt catttccctt ccatgacacc gtgcggggcg gcctgacatc
     5161 cggtgcaatg tcaaatgtcc atcccggcgg tatcagttgc tgcatggctt ttttcagtga
     5221 taacccttta ccgtcccggg gaataaattt cagtgctccc ggagcgctgc tgaccgtgga
     5281 cgatgagggt gcactgacct gagaaggtga cggtgcagca aaaagtggaa cagaaaatgc
     5341 cagcagcggc acaataagaa gtggtttcag tggtgtcata ttatggtcct gtatcgcaca
     5401 ttttttgtgt ttcctgagcc tgtatccggt tttattccgg ctgaactctg ctgcatgaga
     5461 atgcggtgac gttcactttc acactgaacg atttaccggc ctctttttct tttaaaaaca
     5521 gcctgacgca ttcaggctca gtcatcggtg tgttctgaaa acgcacccag gataccggtg
     5581 caccaccagg cttcaggatg gtggcttcaa tccggtattt atgcggtgtg gtgtctgttg
     5641 ttgctgtcat tcatggctcc tgttgccggt ttccggtccc ggaaaaacag gcgacatcag
     5701 caacaagggg aaaaacgcga tgtcgcctga aaggtactgg tgcagcggtg cgttgtacgg
     5761 acgcaggtcc gcacaaccgg aaatacacgg gatgattcgg tcagtgtatt ttcgtgttgt
     5821 gcgcctgtac tgatacggca gggtgtgatg agtgttacct gtggctgcag gcgcggcacg
     5881 tcactcataa cgtagcattc tgtttcctcc tcagcccggc ttcagggcgt tgccgcaata
     5941 cccgattgtt aaagagcctt accggcatgg tgtccggtgt caccgcgcgg ataacgatac
     6001 ggcggtgtct gaagggatga tgctgcggcg ctctgcacat ggcgacgatt aattcttttt
     6061 gtgtttctga aaatttattg ccgggaaatt tctggtgtga cagggtgaat taattctggt
     6121 cagcgacgct cctgagaagg cagagtgcgc tgacatttcg tgaacggagg aatgccatgt
     6181 ccctgaatcc caccgatgaa cagtcagcca tcattcactg gcagggggaa aaactggtgg
     6241 tgaatgcctt tgccggtaca ggcaaaacca gcacactggt gcagttcgcc gcagcctgcc
     6301 ctgagagccg gatgctgtat ctggcctata accgcgcgat tcgtgatgag gcggagcgcc
     6361 ggtttccctt taatgtggag tgcaaaacct cccatcagct ggcgtattcg cggttcggaa
     6421 agcatttccg gcatcgtctg gtgccggggc tgtcagtgac ggatgtagcg agaaaactga
     6481 acacccgcta ctggacgctt gcccgtgttg ccatgaccgc gctgaatcag ttcatctgtt
     6541 cggcggacgt ggcgcccgga atgcagcata tgccgcctcc cgacgagatg aagggcatgt
     6601 cgccacagga tgcgctgcgt gccgtgcaga tcctctggca tgaaatgagc aatccggatg
     6661 gcaattttcc ggtcactcac gacacctatc tgaagctcta tcagctttcc tcaccggatc
     6721 tgtcacgcag gtgggatacg atcctttttg atgaggccca ggatgcgaat ccggtgacgt
     6781 ccgcactggt gctggggcag cggtgcaggg tggtactggt tggcgaccgt taccagcaga
     6841 tttatcgttt tcgtggtgcc agcgatgcac tgtcgcatcc ggcactgaac ggggcggacc
     6901 gtctctggct gacgcaaagt tttcgttttg gtcctgccgt ggccggggtg gccagccagt
     6961 tactgaagcg tgccggcgag agccgtgagg tgaccggcag gggcggggat gatgaagtgc
     7021 tgacggcagg tgaaatgcgt gcgcgcgtga aggcagggaa gatgaccggt cacagggcga
     7081 tactgagccg gacggtggcc ggtgtgattg gcaccgcact gatggcggca atgcgtggca
     7141 ggaaggttta ctgggtgggg ggcattgaag gctacagaac ggaggcactg gaagatcttt
     7201 actggtttca gatggatatg ccggagcgga tgcacagtga cagactgcgg aaggactacc
     7261 ggggttttga cgagtaccgt catattgcga aggtgacgaa ggatgtggaa atgaatcagt
     7321 ccattcgtct gctggagctg tgctttccgc tgccgaagaa actcgaactt ctcagaaagc
     7381 acaccgtcac gaatgaacag gaggcggata tcacggtctc aacggcacac cgcagtaagg
     7441 gactggagtg gtcactggtg gtactcgatg atgattttca ggatatcacc gacccgctga
     7501 taagcgagca ggagcggcgg gatgaaacca atctgctcta tgtggcggtc acccgggcgc
     7561 gcagaacgct ggttctcaat gaactcatgc agcagctgac ggaagctgcc ggcgatggtg
     7621 gtgcacccgg aaacgcgccg gtgcagggga aggaagacga tggcgtggag ggagaggatg
     7681 tttaagcggt tcagacgtat gctgcccggc acaaagggca cggagcctgg cgtggtcgcg
     7741 ccggtgatac cggcagggtt ttatgcgccg ctcacggcag aggcactgtt atcaccagag
     7801 cacagggagc tgaggctcag gcaggtctgg gataacaatc cgatgccccg tgatgtatac
     7861 gacaggatgt gccttgaacc actcagccgg ctgctgctga acacccagaa cgtcccggca
     7921 acacgggaag gacggtggtc ccgtgccggt ggatttggtg atctgacggt actgtacacc
     7981 acgtatgccg ttcgtctggc aagagggtac atgtttccac cggatgcgac cccggaagac
     8041 caggcggctc aggccgcagt ctggcatacg gtgattttct ggtcagcgct gttttaccac
     8101 ctgcccctgc tggcgcacct tgagggggag ctgctgagtg gtcggggctg gcagccaggg
     8161 atttctgtgc ctgacgaacc tttccgtttc cggttcagga agacggcgcc gcagggcacg
     8221 gaagcgcaac aactggcggc cctgactgcc ggcacgctgt taccggacgg cgcaacggcc
     8281 tggcttgtga cggcgccggg ggcgctgcag aatctggccg gggcgctctg gcatcagcat
     8341 cccgggatgg cgctgattcg tgatgtgctg caggaagcgg caaggcagac agagagtccg
     8401 ctgaatacct gcgctgtcac tgcgccggtt actgcggagg cttcagcaga catccgtcct
     8461 gctgacccgg tggtgaccgg cagcacggac agtgtggtgg cagtcactga ggtgcagccg
     8521 gcagaggagg tgaagactga gagcgtgcag acagagactg aaggtggtgg tgagtccgcc
     8581 ggggcgtcag aggtgccgtc cgcacagcag gaactgacgg ttcagacagc gccagtggtg
     8641 acggcagatg atgaagcggt cagtgctgct cctgcggatc aggacacagc aatgctgctg
     8701 agtctttttt cagcagatga gtccgaaact gctgaacctg aaaacagtat taaatcgtca
     8761 gccgatcggg acggccaaag tgacgtaccg gcagtacagg gagaacaaac cagcgttatt
     8821 tatccggagg gcgaagcaga tacggaagag gtggatttta ttacctggct gaaatcgggg
     8881 ttgtcatcgg ggatgattga aataaataca ccttccgcga gagtgcattg tattgcgggg
     8941 tatgtttttt tgtgtgtccc ggatattttt tatctgtatc agaaaatgac gggaagccgt
     9001 cgtgaccgca ggagtgttca ggcgttgttt gaacgttccc gtattcacaa aatccgggaa
     9061 ggtgagcgtt ttttcagcct gcatctgtat actgtacctg acagaacagg gagttataaa
     9121 gctgtaagcg gatatctggt taaaagccgt tatctgtgtg gttataacgc agtgcccggg
     9181 gacagtgaat acattgcttt tccataacag caccgggtca ttcatccgtt cagggagatg
     9241 tattccccta tgaagaaaga tgtcgaaaaa ataacgtggg aggaattact ctttgagtac
     9301 ttccttgaga aaaatcttaa acctgatact gaacacagct accgcaaagt tgtccgtctg
     9361 tttgataagt tcctgggcgg tgattcctgg cccggcgatg tcacaccgag agacattcag
     9421 cgatggcgac gtcatatgct taaagatcag gggcgttcag taaacacctg gaataacaga
     9481 gtgtcacatc tccgaccgct cctggcgctt gggatcaaga aaaagtggtt accacagacg
     9541 gaaaatccgc ttgatggcac ttcagtgccc ggggcatcaa aacagaaaaa aacgctcact
     9601 gataaagaga tggtcaggat ttaccgtaca gttcaggctt ttgctgaagc tgaggccaat
     9661 aagatggtga tctgtgacag gcggcgcaat gcgatgtacc ctgcatggta ctggctgatc
     9721 gtgctggata cgctgcgctt cacggggatg cgttttaacc agctgaaaca tatcaggctg
     9781 aaggatattc atgaggatga ggcggtgatt acccttcagc tggaagggag taaaacccga
     9841 cgtgaatggc gtgtgccgat tgtggaatcg ttacgacttt cactgaaaca tcttattgag
     9901 tgcgcccgtg tcaatggtgc gtcggataat gactatctct ttgatgtctg cctgtacagc
     9961 gagcattaca gtcccgggaa ataccagtac tgtgccaaaa gagcgcatca gtcggtcaaa
    10021 tcttttttcc gcagactgag ccgtgaatgt ggtttttttg tctcgtcaca cagattcaga
    10081 cacacactgg caacaaagct gatggcggca ccggagcgta atctgcacat ggtcaaatcc
    10141 attctggggc acaggagtgt tgccaccacc atggagtata ttgatttttc ggtggaagtt
    10201 gctgctgagg cactggaggc tgaactcggt ttatccacgg atgaggcgac aaaaagacgg
    10261 attaaagagg cgaaaaaacg ccgtgaggcc agtaccggga aaaaggaaaa acaggaaagt
    10321 tctggagggg agctttctga caaggaataa acatgcttga catcggacca gaatggtgta
    10381 agcatttttg cgtatgcgag agcgtgacac ctgttaccag caggtgccac gcttgaggat
    10441 acgatactga aaccacagtg taaccatact tacacggaga tttcgttatg accggatcct
    10501 gtattagacg cccgttaagg atctcatccc ttaaccagcg tactttgatg cgtttggtgc
    10561 ccggaggcgg aatcgaacca ccgacacggg gattttcaat cccctgctct accgactgag
    10621 ctatccgggc aacggggcgc attaaacctg attcgcctcg tctcgtcaat caaatttctt
    10681 caatttactg cagactgcac aatctatcat cactttgccg atattgcacg cattgtcagg
    10741 caaaccaggc ggtccaggcg gcggaaagcg gcattgccag aaggagtgct ggaagcatgt
    10801 tgaccaccgg gaacatttta atgccacaga tgcgtaatcc ggttgccagc agcaattaac
    10861 cgcctacggc gctgaagtct gccatcatcg acggtgtggt cagcggtaaa atcagcgcgg
    10921 cagcccacgc cagcgttaac tggatgatca gtaatgggat actaatcacc gataccgcaa
    10981 tacccagcga gcaggcgaag atcatcgccg taaagaaatc aagaaatgac ttggcgatta
    11041 aaatactcgg atcgccggtc atcccttcgt tcatcgcccc gaagatcccg gtgccgctgg
    11101 cgcaaaacag gacaataatc gcgacataat tctgaataaa agattcatgc gctggcttct
    11161 tacgtgagtg acgaaacaga ttttgtgctt tggcgactgc tgtattgaca cctttctcca
    11221 gcaaacaaat ttcgccaatt agagcgccaa gtagcgtagc taaaaccatc gctggaaggt
    11281 tggcacattt caccaccagt aaaataccaa tccccagcga tgccagacca aaaattgatg
    11341 tcatggagac gcggatacgt tccggtaagc gttggctgag cagtgcgccg agaacgccac
    11401 ccagtaagac tgcactagcg ttgataaaag ggccgatgac cacaagagtt cctgttagct
    11461 gtattgtttg attttcttat tatgaccgtt tggtgccgtc gatggcttgt ttgttcgcaa
    11521 agtcctggct tgcacgcttt agcgaaaggt gccatgattg cgcgaatttt ctcctctctg
    11581 tacggagttt gcccgatgca cgccatctcc ttacattctc tcgcttatcg ccgtttcgcg
    11641 cgaaacgttt ccctttctat gttactgctc atgcggtgaa gttaacgcac gctcactgca
    11701 ggacaacagt aaaatcagag cgtttctgct tttactgatg tctggcggtc ggagctggtg
    11761 accagtttga cccatatctc atggggcagg gttttccacc ttgtccggta ttcttacttc
    11821 cccgaaacgg gtttgcgctt atgaaatcaa tgaatattgc cgccagtagt gaactggtat
    11881 cccgactttc ttctcatcgt cgcgtggtgg cgttgggaga tactgatttt acggacgtcg
    11941 cggcagtcgt cattaccgct gcggatagtc gcagtggcat tcttgcgttg cttaagcgca
    12001 ccggttttca tctaccggtg tttttgtatt ccgaacatgc tgttgaatta cctgcgggcg
    12061 ttacggcggt aatcaacggc aacgagcagc agtggctgga gctggaatcc gcagcctgtc
    12121 agtatgaaga gaatttgctg ccaccgtttt atgacacgct gacgcagtac gttgagatgg
    12181 gcaacagcac ctttgcttgc cctggacatc aacatggtgc gttttttaaa aagcatcctg
    12241 ccggacgcca tttttacgat ttctttggtg agaacgtctt tcgcgccgat atgtgtaacg
    12301 ctgacgtaaa attgggcgat ctgcttattc atgaaggatc ggcgaaagat gcgcagaaat
    12361 tcgcagccaa agtctttcat gccgataaaa cctattttgt gctgaacggc acatcggcag
    12421 cgaataaagt ggtgacgaat gcgctgttaa cgcgtggcga tctggtgctc ttcgaccgta
    12481 acaaccataa gtcgaatcat cacggcgcgc tgattcaggc gggggcgacg ccggtctatc
    12541 tggaagcttc acgcaacccg tttggtttca ttggcggtat tgatgcgcac tgttttaatg
    12601 aagagtatct gcgccagcaa attcgcgacg ttgcgccaga aaaagccgac ctgccgcgcc
    12661 cgtatcgcct ggcgattatt cagctgggaa cctatgacgg cactgtctat aacgcccgtc
    12721 aggtgatcga taccgttggg catctgtgtg attacattct gtttgattcc gcgtgggtcg
    12781 gttatgaaca atttatcccg atgatggcgg atagctcgcc gctgctgtta gaacttaacg
    12841 aaaacgatcc ggggatcttt gtgactcagt cggtgcacaa acagcaggcg ggattctcac
    12901 agacgtcgca gatccataaa aaagataacc atatccgcgg acaggcgcgt ttttgcccgc
    12961 ataagcggtt gaataacgcc tttatgctcc atgcttctac cagccctttc tatccgctgt
    13021 ttgctgcact ggatgttaac gccaaaattc atgaagggga gagtgggcgt cggctgtggg
    13081 ctgagtgtgt tgagataggg attgaagcgc gcaaggctat tcttgcgcgc tgtaagctgt
    13141 tccgcccgtt tatcccgccc gttgttgatg gcaaattgtg gcaggattat ccgacatcag
    13201 tgttagccag cgaccgccgt tttttcagtt ttgagccggc ggcgaagtgg cacggctttg
    13261 aaggatatgc cgcggatcag tattttgttg atccgtgcaa gctgttactc actacaccag
    13321 gtatcgatgc cgaaaccggc gaatatagcg actttggcgt tccggcgacg attctggcgc
    13381 actatctgcg tgagaacggc attgtgccgg agaagtgcga tctcaactcc attctgtttt
    13441 tattaactcc ggcggaaagc cacgagaagc tggcacaact ggtggcgatg ctggcgcaat
    13501 ttgaacagca tattgaggat gactcgccgc tggttgaggt gttgccgagc gtttataaca
    13561 agtatccggt gcgctatcgc gactacaccc tgcgccagtt gtgtcaggag atgcacgatc
    13621 tgtatgtcag tttcgacgtc aaagacctac aaaaagcgat gttccgccag cagagtttcc
    13681 cgtcagtggt gatgaacccc caggatgcgc atagcgctta tattcgcggt gacgtggagt
    13741 tggtgcggat tcgtgatgcc gaagggcgaa ttgcggcaga aggggcgttg ccttatccac
    13801 ctggcgtgct ttgcgtggta cccggggaag tctggggtgg ggcggttcaa cgttatttcc
    13861 ttgcactgga agaaggggtg aatttgttgc cgggattttc gccggagctg caaggtgttt
    13921 atagcgaaac cgatgcggat ggcgtgaaac ggttgtacgg ttatgtgttg aagtaagaat
    13981 aaaaaaaacg ggtcaccttc tggcgacccg tttttctttg cgtaattagt ggctaaccgt
    14041 ctgtgtgcct gtcgggacac gaacgtgttt atatttgaac atcgccatga acgcgaaggc
    14101 cagaaccacg gagtaaccag cgaaaatcaa ccatacggtc tgccagtcgg taatgccgtt
    14161 ttgggtgtac atctcaacaa ctttaccgct cacgatgccg ccgaggatac agccgaagcc
    14221 gttagtcatc atcaggaaca tcccttgtgc actggcgcga attgccgggc taacttcttt
    14281 ttcgacaaac accgaaccag agatgttgaa gaagtcgaat gcgcaaccgt aaacgatcat
    14341 cgacagtacc agcagtacag taccgaacgg agtcgggtcg ccgtaagcaa acagcgcaaa
    14401 acgcaggatc cacgccacaa tactgatcat cattacgttc ttaataccgt agcggcttaa
    14461 gaagaacggg atggtcagaa tgaacagggt ttcagagatc tgcgaaatcg acatgatgat
    14521 tgacgcatgc tgcacaataa agctgctggc aaacatcgga tctttgtcga agctgtgcag
    14581 gaaggtatta ccgaacatgt tggtaatctg cagttccgcg cccagcagca ttgagaagat
    14641 aaagaagatt gccatacgct tgtttttaaa cagcgcgaat gcatcgaggc ccagcagggt
    14701 tgtccagctc tgattcgctt gctgtttagc aaccggaata tgcggcagag tcagggtaaa
    14761 cagaaccaga atggcggaaa gtgctgcgcc aatatacagc tgcatgtggc ttaattcgaa
    14821 gccagacagg ctcaccaccc acattgccat gataaagccg atggtgcccc agatacggat
    14881 tggcgggaag tcagtaacga tatccatccc ggcattttgc aggcgatagt aagagatggt
    14941 gttgattaac ccaagcgttg gcatataagc aaacgagtta atcaatatca caaggaacat
    15001 cgcttccggt gtcgtgacct gtgccgccat gaacagcgtg atagcgccaa tggtgtggca
    15061 aatggcatat acccatttcg cacttaacca tttgtcggcc acaatcccca gcagcgcagg
    15121 cataaagacc gctgcgatac ccagtgagct ataaactgcg ccaatagaag caccgtcaaa
    15181 cttcagggta acaaacatat aggagccgag ggtcgtcagc caacttcccc acagacagaa
    15241 ctgcagaaaa gagaggattt tcagctgcag cttaagattc atgttaattt cctcacatcg
    15301 tgatgcggat gagtgtttga aaaagcacat ttgaccgtgc gagatgatgg gattctatca
    15361 acttcgcgga ctggtttttt gttacctgtg gcaaataatt gcaaacattc ccaatttgca
    15421 aaacgaaatt gtgatgtgga taacattttt tcccctgagc atcgtcaggg gcggttaatg
    15481 gaattatcgg cggcggtagg atttttgcgc ggtattcact ttataaagat aacggcgaga
    15541 ttccgcagag ggatggcggg tcgtcagcgt ctgataaaca tcgcccggcg tcatggtgtt
    15601 aataatattg gcagcctgaa tcttatcatt cgaaaagact cgcagcacgc tgcctgcgcc
    15661 gccgttatag gcggtgatga cggcataacg ccgcgatgtt gggttatcaa ttccgccgag
    15721 ataaacattg ttcagcatcg ccagatacgc ggtgccggta tcaatattgc tggcaggatc
    15781 aaacaagaaa ctgcggctcg gcgtgccgga tttcccctgc gagcggaaca catctttccc
    15841 ggcagtatgt tgtaccacct gcattaatcc cagcgcatcg gaacggctga ccgcatacgg
    15901 gttaaaggaa gattcggtct gcataattgc cagaatcagc gactcatcaa cgccatattt
    15961 ccgtgacgcc tggcggacca tgccgagata tttgtgcgca cgtttatcaa ggtggttcgg
    16021 caccatgtta atggtgacgc tgtagatgat acgcagcccg ttgctgcggc tcttcagacg
    16081 gtttttcagc agataatccg cgaagttgct tgcgcgacct tcccagcgaa tcggctgccc
    16141 ggtgttgtcc accacctgac cgtaaaggaa aggttctttc gaaatcgtaa tatcatcaac
    16201 gtcggaatag agatcgaccg aactcggatc gtcacccatc aataacgttt tgataattgc
    16261 ccggcgcaaa tgcgcggcag gttctgtccc ggcgatggtt tcgatagtaa tcgtaccgtc
    16321 atcgaagttg atgtggctgc gggtctgata ttgatcggtg tatttcacgt agtccttagg
    16381 accagcgatc accacctctt tgaagcccca gatgttctca atattgtggg caaattgccc
    16441 catcagaata tcaaaaccgt tggtatcttt gacccaggct tcgttatagg tatcgccttt
    16501 tttggtcgtc gaacaggaga tgagcaacgg cgcaatcaaa gccagcgcga gatatttttt
    16561 catcattccg ggggcgtgtt gtgtttgttt ttatgcaagt tgccggaggc gtgctccggc
    16621 actgttttta ttttttatct tccggcgtat agccctcgat atgcacctct ttaccctcga
    16681 acaggaagtt gaccatctcc tgctcaagca gcttgcggtg ctcggcattc atcatgttga
    16741 gtttcttttc attaatcagc atggtttgct tgtgctgcca ctgcgcccag gcttctttgg
    16801 agatctcgtt atagatgcgt tttcccagct cgccggggta cagctgaaaa tcctgacctt
    16861 ctgcttcacg ttgcaggaaa gtacaaaaaa tcgttctgct cataaatcat cctctttatc
    16921 gactcacgcg ctaaaccggc gcgccagtgc gtaactgctg taacaaacgc tccacgggag
    16981 ccgccaggcc aactgacggc ggttgcgcta agttatacca gagcgcattg ccttcatcca
    17041 tgcagccggt gaatgacgac acgggaagcc acataggcac aatatctaag tggaaatggc
    17101 tgaaggtatg ccgaaacgcg gtcagttgcg tcaggttatc ggcagcaatc tgccgttgcg
    17161 ccagccactg ccgcaaactt tcttcgtcgg caaactgcgg gaaacagtat aaaccgcccc
    17221 acaatccgct cggcggacgc tgcgccagca atacttcatc ttcgtgctgt aatagcaaaa
    17281 agtagccggt gcgctccggc agcgtctgtt tcggtttttt gcccggataa agcgcccagc
    17341 tattgttggc ggcggcaata catccgtttt gtagcggaca gagcgaacat ttcggtttcg
    17401 agcgcgtaca aatcatcgca cccaaatcca tcatcgcctg attaaaccgt tccacgccaa
    17461 ccgcgggcgt cacctgctcg ctcaaactcc ataatttatt ctcgacctct tttttcccag
    17521 gccagccgct tacagcatag cagcgcgcca gcacgcgttt gacgttaccg tcgagaatcg
    17581 gaaagtgctt acccagagaa agcgagagaa tcgcgcctgc ggtggaacgc ccgacgcccg
    17641 gcagtgctgc aacttcctca aaggtttccg ggaatttacc gccgtgtaag gtcgccactt
    17701 gttgtgccgc tttatgcaga ttgcgcgcgc gggcgtaata gccaagcccg gtccacaagt
    17761 ggagaacttc gtcgagcggc gcattggcga gatcggtcac cgtcgggaag cgcgccataa
    17821 agcgttcaaa ataggggata acggtcgcaa cctgagtttg ttgcaacatc acttctgaga
    17881 gccatacttt gtagggcgtc ttgtcaattt gccagggcag agtttttcgc ccgtatttat
    17941 cgtaccagtc cagaacctgg gctgaaaatt gcgacgcttg catggtcacc gaattcactg
    18001 ttgttggggg caagattgca gcacagcgac ggcagggtgt aaaccggaac tttccgcagc
    18061 cacggcctta tcatgcttgc atccggcaac taactttgga taatgcccgt tttcagaaca
    18121 ctttcacaag cgactaaacc tttatgaaaa acgacgtcat ttcaccggaa tttgatgaaa
    18181 acggccgccc actgcgccgt atccgtagtt ttgtgcgccg ccaggggcga ctgaccaaag
    18241 gccaggaaca tgcgctggaa aactactggc cggtgatggg cgttgagttc agcgaagata
    18301 tgctggattt ccccgcgctt tttggccgtg aagcgccggt gacgcttgag attggttttg
    18361 gcatgggggc gtcgctggtg gcaatggcta aagatcgccc tgagcaggac ttcctcggca
    18421 ttgaagtgca ttcaccgggc gttggtgcgt gcctggcttc tgcgcatgaa gaaggtttaa
    18481 gcaacctgcg cgtgatgtgt cacgatgcgg ttgaagtgct gcataaaatg attcctgaca
    18541 attcattgcg catggtgcag ctctttttcc ctgacccgtg gcacaaagcg cgccataata
    18601 aacgccgtat cgttcaggtg ccgtttgccg aactggtaaa aagcaaactg cagctggggg
    18661 gcgtattcca tatggcgacc gactgggaac cttatgcgga acatatgctt gaagtgatgt
    18721 cttctattga cggttataaa aacctgtcag agagcaatga ttacgtaccg cgtccggcat
    18781 cacgtccggt gacgaaattt gaacaacgtg gtcatcgtct tggtcacgga gtatgggact
    18841 taatgttcga gagggtgaaa taatggcaaa gaaccgtagc cgtcgtctgc gtaaaaaaat
    18901 gcacatcgac gaattccagg aattaggatt ttcggtggca tggcgattcc cggaaggtac
    18961 atcggaagaa cagattgata aaaccgttga tgattttatt aacgaggtta tcgaaccgaa
    19021 caaactggcc tttgacggca gcggttatct ggcctgggaa ggtctgatct gcatgcagga
    19081 aatcggcaaa tgcaccgaag aacatcaggc gattgtgcgt aagtggctgg aagagcgcaa
    19141 actggatgag gtacgcacca gcgaactttt cgacgtttgg tgggactaag aaagcatacg
    19201 ggcgatgaca aatgcaaaac tgcctgatgc gctacgctta tcaggcctgg aaagatgcac
    19261 gatcgagtag gcgggataag gtgtttacgc cgcatccggc atggaaaacg cgtactttgt
    19321 tatcaatctg gggccagcaa atgctggcct gattttttct tgagggaaga ctatgatgcg
    19381 caaaatgctg ctggcggcag cactttcagt gacggcaatg accgctcacg ccgactacca
    19441 gtgcagcgtc acgccgcgtg acgatgtgat tgtcagcccg caaaccgtgc aggtgaaggg
    19501 cgaaaacggc aatctggtga tcacgccaga cggcaacgtg atgtataacg gtaagcaata
    19561 ttccctgaat gccgcccagc gcgagcaggc gaaggattat caggctgaac tacgcagcac
    19621 gctgccgtgg attgatgaag gcgcgaaaag ccgcgtcgag aaagcccgta ttgctctgga
    19681 taaaattatc gttcaggaga tgggcgaaag cagcaaaatg cgcagccgtc tgaccaaact
    19741 tgatgcgcag ctgaaagagc agatgaaccg cattattgaa acgcgcagcg atggcctgac
    19801 gtttcactat aaagccattg atcaggttcg cgccgaaggc cagcaattag tgaatcaggc
    19861 aatgggtgga attttacagg acagcattaa tgaaatgggc gcgaaagcgg tgctgaaaag
    19921 cggcggtaac ccattacaga atgtgctggg aagcctgggg gggctgcaat cctcaatcca
    19981 aaccgagtgg aaaaagcagg aaaaagattt ccagcagttt ggcaaagatg tttgtagccg
    20041 cgttgtgact ctggaagata gccgcaaagc cctggtcggg aatttaaaat aatcctctat
    20101 tttaagacgg cataatactt ttttatgccg tttaattctt cgttttgtta cctgcctcta
    20161 actttgtaga tctccaaaat atattcacgt tgtaaattgt ttaacgtcaa atttcccata
    20221 cagagctaag ggataatgcg tagcgttcac gtaactggag gaatgaaatg gagtttttca
    20281 aaaagacggc acttgccgca ctggttatgg gttttagtgg tgcagcattg gcattaccca
    20341 atatcaccat tttagcaacc ggcgggacca ttgccggtgg tggtgactcc gcaaccaaat
    20401 ctaactacac agcgggtaaa gttggcgtag aaaatctggt taatgcggtg ccgcaactaa
    20461 aagacattgc gaacgttaaa ggcgagcagg tagtgaatat cggctcccag gacatgaacg
    20521 ataatgtctg gctgacactg gcgaaaaaaa ttaacaccga ctgcgataag accgacggct
    20581 tcgtcattac ccacggtacc gacacgatgg aagaaactgc ttacttcctc gacctgacgg
    20641 tgaaatgcga caaaccggtg gtgatggtcg gcgcaatgcg tccgtccacg tctatgagcg
    20701 cagacggtcc attcaacctg tataacgcgg tagtgaccgc agctgataaa gcctccgcca
    20761 accgtggcgt gctggtagtg atgaatgaca ccgtgcttga tggccgtgac gtcaccaaaa
    20821 ccaacaccac cgacgtagcg accttcaagt ctgttaacta cggtcctctg ggttacattc
    20881 acaacggtaa gattgactac cagcgtaccc cggcacgtaa gcataccagc gacacgccat
    20941 tcgatgtctc taagctgaat gaactgccga aagtcggcat tgtttataac tacgctaacg
    21001 catccgatct tccggctaaa gcactggtag atgcgggcta tgatggcatc gttagcgctg
    21061 gtgtgggtaa cggcaacctg tataaatctg tgttcgacac gctggcgacc gccgcgaaaa
    21121 ccggtactgc agtcgtgcgt tcttcccgcg taccgacggg cgctaccact caggatgccg
    21181 aagtggatga tgcgaaatac ggcttcgtcg cctctggcac gctgaacccg caaaaagcgc
    21241 gcgttctgct gcaactggct ctgacgcaaa ccaaagatcc gcagcagatc cagcagatct
    21301 tcaatcagta ctaatcgcct cgccccggta tcgtgccggg gctttttcac ttcagactca
    21361 cgtccattgc caattttaat taccctaatg ataatcaccg gaataaatta ttccgcgtga
    21421 ggtttttcgg gtgaaaaagc aatggattgt tggcacggca ctgcttatgt tgatgactgg
    21481 taatgcctgg gcagatggcg aaccgccaac tgaaaatatc ttaaaagatc aattcaaaaa
    21541 gcagtatcac ggcattctca agcttgatgc tatcacctta aaaaatcttg atgctaaggg
    21601 taatcaggcc acctggtcag cggaaggcga tgtctcttcc agtgacgatc tctatacctg
    21661 ggtcggtcag ttggcagatt acgaactgct cgaacagacc tggacgaaag ataaaccggt
    21721 aaaattctcg gcgatgttaa ccagtaaagg aacgcccgcg tctggctggt cggtgaactt
    21781 ttactctttt caggcggcag ccagcgatcg tgggcgggtg gtcgacgata tcaaaacgaa
    21841 taataaatat ctgatcgtga atagcgaaga tttcaattat cgctttagtc agcttgaatc
    21901 tgcgttgaat acccagaaga actcgattcc tgcgctggaa aaggaagtga aagcgcttga
    21961 taagcaaatg gtggcggcgc agaaagcagc ggatgcatat tggggaaaag atgcaaacgg
    22021 taagcaaatg acccgcgaag acgcctttaa aaaaattcac caacagcgtg atgagtttaa
    22081 taaacagaac gatagcgagg cgttcgcggt taaatatgac aaagaggttt atcaaccggc
    22141 gattgcggca tgtcataaac agagtgaaga gtgttatgaa gtgccgattc agcagaaacg
    22201 agatttcgat atcaacgaac aacggcgaca gacttttctg caatcacaaa aacttagccg
    22261 taaattgcag gatgactggg taacgctcga aaaagggcaa tatccgttaa cgatgaaagt
    22321 ttcagaaata aacagtaaaa aagtcgcgat tttgatgaaa attgacgata tcaaccaagc
    22381 taatgaacgc tggaaaaaag ataccgaaca actgcggcgc aatggggtga ttaagtaagt
    22441 ttgatatagt gcacgatttg tagcaggatg cggcgttgac cccgcatccg gcaatcaacg
    22501 tctgatgcga cgcttgcgcg tcttatcaga cctacaaccg ctgccacatg tcggctggat
    22561 aaggcgttca cgccgcatcc ggcaatacaa gtttactcag ccagaaaaag ctccagcagc
    22621 gaatttaaaa acagcttccc atgttccgtt atctgccagt aatccgcaca ttcggtgaga
    22681 taaccctggg caatcgcctc gtctaactgt gggcgaatca catcttcgca aagcccggta
    22741 tacgcaataa actccacgcg cggcgcggcc tccagcagac ggaagcgatt cataaagaac
    22801 tcaaacggct tatctgtggc ttcgacatca cgctggcttt ccagatacct tccttgcata
    22861 aaaccacgcg gatgacgcgt tttggtggta cgcagaatgc gcccatccgg gaaggtcact
    22921 ttgccgtgtg cgccgcagcc aataccgatg tagtcaccaa agcgccagta gttgagattg
    22981 tgctggcact gataaccggg tttggcgtaa gcggaagttt catattgctg ataacccgct
    23041 gcggttaata actgatgccc ctgttcgaat atatcccaca acgcgtcatc gtccggcagc
    23101 accggtggtc gcgaaccaaa cagcgtattg ggttcgatgg tcagttgata ccaggaaaga
    23161 tgcggcggat tcagttcaat ggcctggcgt agatcgccaa gcgcctcttc cagtgattga
    23221 tccggcagcc catgcatcaa atcaaggtta aagctacgta accctaaacc gctcgccagc
    23281 ttcgccgcgc gtttcgcttc ttgcgggcca tgaatacgcc caagtcgttt cagcttttct
    23341 tcgctaaaac tctgcacacc aatagagatg cggttcacac cagcacgctg ataatcgaca
    23401 aagcgatcgg cttctaccgt gccagggttc gcttccatag taatttctgc atccgctgcc
    23461 agcggcaaac gcgcacgcac gccgtccagc agcgtttgca tcgccgggcc ggaaagcagg
    23521 ctcggcgtac caccgccaat aaagattgtc tttacttcac ggccctgagc gtaagccaca
    23581 tcgttgtcca gatcgttaag cagatgctga acataatcgt cgtgcggcac ttctcctttc
    23641 aacgcgtgag agttgaaatc gcagtacggg catttctgca cgcaccacgg gatgtgaatg
    23701 tagagactca gcggaggtaa tttaaccatt acgtaaagcg tccagcagca gtttcaacgc
    23761 ctgaccacgg tgggaaatgg cgctcttttc ttcgcgggtc agttcggcgg cggttttccc
    23821 ttcggaaggt acgaagaaga ttggatcata accaaagcca ccagtgcccg ctggttcacg
    23881 agtaatcacg cccggccagc taccgtggca taccagcgga gtgggatctt ccgcgtgacg
    23941 cagatatacc agcacgcagt ggaaacgcgc ctgacgttgg tcgtccggta cgtctttcat
    24001 tgtttccagc agtttttgca gattcttttg atcggtcgcg tcttcaccgg aataacgcgc
    24061 ggagtaaatg cccggcgcgc cgccaagcac atctaccgcc agaccagaat cgtcggcaat
    24121 tgccggtaaa gcggtcactt ttgccgcatg gcgcgctttc agaatcgcgt tttcgataaa
    24181 ggtcaggccg gtttcttcag cggaatcaac gccgaggtct gtttgggcca cgatatcaag
    24241 accgaagtcg ctaagcagcg acgccagctc acgcacttta ccgacattgc cggttgcgag
    24301 gacaactttt tgcataggat acctaattaa ttaacgccgc gatttctggc gggatttgtt
    24361 gcggattaat gattttaatt tgtttgtggc ggccaagttc gcctttttca atcaccacct
    24421 ggcttttggc aacccggaat tgcttaccga gaaacttcac cagatgactg ttggcctggc
    24481 cgtcaaccgg cggcgcggta atggcgactt taacttcgtc gccatgtaaa ccgacaatag
    24541 aatcacggct ggctttcggc tgaatataga gccgtaaaac cagaccgtca tcattaactg
    24601 ttacggcatt cataacgcca tccacagccc tggcagcagc atatttccgg ttgcctgtaa
    24661 tacttctgcg acacccatat tgatgacata cagcagcaga acgaggatca tcggcgagaa
    24721 atcaatccca cccattgccg gtagcaggcg gcgaatcggg cgcagcagcg gatcggccag
    24781 ctgaatcagc acgtattcaa tcgggctacg cccctggctt acccagctca taatcgccat
    24841 caccagcagg acccagaaaa tcagcaggcc gatggttttc agcagaatca gtaaaccggc
    24901 aatccagatg attggcagga aggtcaccac tttaaacagc acgatggctt tgataaaact
    24961 gagaatatag gcaaccagca gcgaggcgct gtcaattggc cccattgccg gaataacgcg
    25021 gcgcagtggc ccgataattg gctgcgttac cttcactaca aactgtgaga aggggttgta
    25081 aaaatcacaa tgagcccact gcatccagat gcgtaataac agcaccatgg tatacagctc
    25141 aatgaccgtt gaaagcagga aagtcaacgt attcatggcg ttcctttaat tccttatttt
    25201 ttagagtaat cacgcgcacc aaaaattgca gtgccgatac gaaccatcgt gctacctgcc
    25261 gcaatggcgg cttccatatc gtccgacatt cccagagaga gcgtgtcgat atgcgggtag
    25321 cgcgttttca gtccggcaaa tgctacagcc atttggcgtg caacttcaaa ctgccttaca
    25381 tattctgact caggcgcagg gattgccatc aacccgcgca gacgtaaacg cggtagttca
    25441 gcgaccgcag ctgccagctc gtccagttca gccagttgaa tcccggactt actgttttca
    25501 tcactaatgt taatttgaat cagaacgtta agagggggaa gttctgccgg gcgctggtcg
    25561 ttgagacggg tagcgatgcg caaacggtcg atggtatgac accagtcgaa atgctctgcc
    25621 accaggcggc ttttattaga ctgcaacggg ccaataaaat gccattctaa tcctgttacg
    25681 cccagttcct gaaagtggcg aattttatct accccttcct gaacgtagtt ttcaccaaat
    25741 tgacgctgcc cggcatcaat ggcttctgcg atggcgctcg caggttttgt tttactgact
    25801 gcaagcagcg taatttcttc tggagaacgg ccgcaacgcg ttgcagccgc tgagattttg
    25861 tcccggacct gtgccaggtt atgcgcaata tcgttcattt tccgaggatc ttagtatgaa
    25921 tatggaagaa attgtggccc ttagtgtaaa gcataacgtc tcggatctac acctgtgcag
    25981 cgcctggccc gcacgatggc gcattcgcgg cagaatggaa gctgcgccgt ttgatacgcc
    26041 ggacgtcgaa gagctactgc gggagtggct ggatgacgat cagcgggcaa tattgctgga
    26101 gaatggtcag ctggattttg ccgtgtcgct ggcggaaaac cagcgattgc gtggcagcgc
    26161 attcgcgcaa cggcatggca tttcgctggc gttacggctg ttaccttcgc actgcccgca
    26221 gctcgaacag cttggcgcac caacggtatt gccggaatta ctcaagagcg agaatggcct
    26281 gattctggtg acgggggcga cggggagtgg caaatctacc acgctggcgg cgatggttgg
    26341 ctatctcaat caacatgccg atgcgcatat tctgacgctg gaagatcctg ttgaatatct
    26401 ctatgccagc cagcgatgtt tgatccagca gcgggaaatt ggtttgcact gtatgacgtt
    26461 cgcatcggga ttgcgggccg cattgcggga agatcctgat gtgattttgc tcggagagct
    26521 gcgtgacagc gagacaatcc gtctggcact gacggcggca gaaaccgggc atttggtgct
    26581 ggcaacatta catacgcgtg gtgccgcgca ggcagttgag cgactggtgg attcatttcc
    26641 ggcgcaggaa aaagaccccg tgcgtaatca actggcaggt agtttacggg cagtgctgtc
    26701 acaaaagctg gaagtggata aacaggaagg acgcgtggcg ctatttgaat tgctgattaa
    26761 cacacccgcg gtggggaatt tgattcgcga agggaaaacc caccagttac cgcatgttat
    26821 tcaaaccggg cagcaggtgg ggatgataac gtttcagcag agttatcagc accgggtggg
    26881 ggaagggcgt ttgtgacgtt gccatgcccg atgcgcaagc ttatcgggcc tacaaaggca
    26941 ataaatgtct gatgcgacgc taacgcgtct tatcagacct atagccgctg ccagatgtgt
    27001 gctggataag gtgttcacgc cgcatccggc aataagacgc gatgatatta tttacactcc
    27061 gccaggcgtt taaatcgcct taatatccct gctcgaaata gctttcgaga ataataaccg
    27121 cagaggcaga gtcaactttg cctttgttga gcgcccgata gccgccctgt tcaaacagac
    27181 cggaacgggc ttccacagtg ctaagacgct cgtcatggag ctttacttca acaccgaaac
    27241 ggccatgaat acggttggca aatttacgcg ctctggcagt caatggttgc tcggtgccgt
    27301 ccatattcag cggcaaaccg acgatgattt cgtccggctg ccactctttc agtaaacgct
    27361 cgataatgtt ccagtccggc gtaccgtcct gtgctttaat tgcaggcaaa gggcgagcgg
    27421 tgccggtaat gcgttggccg accgctacgc caatgctttt ggtgccgaag tcgaaggcga
    27481 gtaaggttcc actcatcagg cgtgtcctgc cacaccaggc atggtgagaa tatccacacc
    27541 aatcagtttt gccgcctcgc gccagcggtc ggcaatcggc gttttgaaca gaatattcag
    27601 atctgccggg gccgttagcc acgcgttatc gagaatttct tgttccagtt gacctttctc
    27661 ccaggaggca taacccagag ccaccaatac gtcagacggt tgtttatcgg tgccgagcgt
    27721 ttccagcaca tcgcgggaag tggtcattac cgtgttgtct gaaatgcgaa tgctggaagc
    27781 aaaattggag ggcggagtat gcaaaataaa cccgcgatct tcagccagcg gaccgccgag
    27841 cataaccggt ttatccagac ggattgattc atcacgcggc tccggcgtga tcttcagctt
    27901 ttccagaatc ccttcaattt tgagattttc cagcggcttg ttgacgatga tccccattgc
    27961 accattggta ttatgttcgc aaatgtagac cacggaacga cggaaaatcg gatcctggag
    28021 agcaggcatg gcaataagaa agtgatgctg taaattcatt gtcagaggtt ctgtttcctg
    28081 gttcaaaaaa gcaacagcgc ccagtatgcg tggaaagaag ggcgctgtca ctcagagtct
    28141 caacgagatc cttctcgcta aggtgggtta ctgctgctgt aaacgtgctt cgatggcatc
    28201 cattaacatt ccggtgatcg acaccggaaa ctctgcttca atctcacgaa tacaggttgg
    28261 gctggtgacg ttaatttcag tcagacggtc gccgatgata tccagaccaa caaaaatcag
    28321 ccctttttct ttcagcgtcg gcccgatctg acgggcgatt ttccagtcac tttccgtcag
    28381 cggacgaggt tcaccgcgac caccggcagc cagattgcca cgggtttcgc ccccctgcgg
    28441 aatacgcgcc aggcagtacg gtaccggctc gccatccacc accagcacgc gtttgtcgcc
    28501 atctttaatg gctggcaggt aattttgcgc catgcagtag cgagtgccat gctcagtcag
    28561 ggtttcggca atcacgccga ggtttggatc gccttctttc acgcggaaaa tcgacgcgcc
    28621 gcccataccg tccagcggct taagaatgat gtcgctgtgt ttctcccaga acgcttttag
    28681 ctgcgcttta ttgcgcgtaa ccagcgtttc tggcgttaag tcagagaacc aggcggtaaa
    28741 cagtttctcg ttacagtcgc gcaggctctg cggcttgtta acgatcagcg tccctttctc
    28801 ttcggcacgt tccagaatat aggtcgcgta gataaactcg gtatcaaacg gcgggtcttt
    28861 acgcatcagg atcacatcga gatcggccag cggcagatcc tgttcaccga cgaacgaaaa
    28921 ccactcttcg tagttctgct tcacgttcag cgtgcgggta tgggcgcggg cttcaccatt
    28981 gatcagatac agatcgccca tctccatata gtgaagttcg taaccacgac gctgtgcttc
    29041 cagcaacata gcaaaactgg aatctttctt gatgttgatg tttgcgatgg ggtccatcac
    29101 gatgccgagc ttgatcatta ttcttctccg ttagcccaaa tcgccaaatc gtacttgtag
    29161 cgcggtaatg gcggtgagcg cagttgtctc tgtacgcaaa acgcgaggtc ccaacaggat
    29221 atcagtaaat tgatagcggg cagtcatggc aatttcatct gccgataaac cgccttccgg
    29281 gccaatcagc aggcggacgc gttcaaccgg taacggcaac gtattgatgc tgttactggc
    29341 gcgcgggtga agattcagtt tcagtccttc atcctgctct gcacaccagg cttccagatc
    29401 catcgctgga cggatttccg gcacccggtt acgaccacac tgctcacagg cagcaattgc
    29461 aatcttctgc cactgctgaa gcttcttgtt cagacgttca ctatccagtt taacgccgca
    29521 gcgctcagaa aaaagtggcg taatgaggct tacaccgagt tcgatcgatt tctggatagt
    29581 aaattccatt ttttcaccac gcgacatcac ctgaccgagg tgaatatgca gcggagattc
    29641 gcgatcgtcg atctggcctt ccagcacctt cacttccacg ctttttttgc tggcgctggt
    29701 aatttcggcg tcaaagacct ggttgctacc gtcaaacaat tgcaacgcct gccccggccc
    29761 catgcgcagt acgcgcccga tatggttggc ggcatcttcg caaagcgcga tgtgagaatg
    29821 gctggtcagt ggttcaggat gataaatgcg ggggatacgc atagttaaaa atccgcgtcg
    29881 tctccccacg cggttgtacg cgtggggtag gggttaacaa aaagaatccc gctagtgtag
    29941 gttagctctt tcgcgcctgg caagcgcgtt gcacatacgg gttatgattg ccctgcacct
    30001 tcgcgatgcg ttcatcgcgc tcgcactccc agtcggtaac cggatacatc ttgttccatg
    30061 cgttgaacag ctgcgtttgc tggcgagaga gtgtcaggtt gtattggtcg cgcatataga
    30121 agtaggtgcg cgcaatggca ccgcgtgcac gcgctggtgg ttcggcagct ttttctttga
    30181 aatcgacctt catggcgcat tgaccgtact ggccttcacc gccattccac tggctgtaca
    30241 taaagttgcc gcgatcgcca ttcacctcac cgactgacgg ctgcaggtta tgcatatcgc
    30301 tttccatctt gcgatagacc ggatctttag tgcagttttt acgtccaccg tcctgccagc
    30361 actggcgctg gtgaccgaac tgccaggcgg gaacgacatg ttcccactct acgcggctgg
    30421 cgcggttttc atttttgcgc acctgatagc cgcacgattg cagatcaaca acgccttttt
    30481 tgccctgcca gttaatttta catccgcaat aaaacgtacc gggcgcgtca gcgtggactt
    30541 ttaccgccgc ggctttcgcc tgagaaaaac tattgatacc ttcggccaac gccgggccgg
    30601 aaaatgctgc gctcagtacc accgcagcaa tagacaaata acggtacatc acttactccg
    30661 tgttaaaacg agccagcaac gtagcgaaag ctgttttggt atgcaatcag ccagatcagg
    30721 aaagttcctg atagttcaga tggttatttc gcaaccagct gttcaccgca gtgaacacag
    30781 cgatagacgg cctcgccacg aactacgcga ttatggcggc gtacggtaag ctgatgctcc
    30841 tggcacttgc agcggtaggg gaaggtgttg cgacgcacgg attgcagttc gaactgatgc
    30901 gtacgacggg cgggaacacc cagcacgttt tccatcatcc acttccactc tttgccatgt
    30961 ggcgctacgc ggccgaaatg tttccatacc agcaaatgtg ccagttcgtg cggtaccact
    31021 tcttcaataa aagcttcact gttttccaac agcaaaacgg gattgaggcg aatttcatag
    31081 ctttccagcc aggccgttcc ggcggaggtt ccgcgctggg tgtaagagag ttttggctcc
    31141 gggtagttac gccctagctt caggttggcc tgggcgagtt tttcccgcag gcgacgcata
    31201 acggcctgtt ggatggcgat agggagacgg gatgttttca tagcggcaga ggatagagcg
    31261 aagaaaagat gactgcaaga ggtggcttcc tccgcgatgg gaggaagctt ggggagatta
    31321 atcgtgagcg cctatttcgc gcagtttacg acctttcatc agattacgtt caatatgttc
    31381 cagcgaaacg tgtttggttt ccggtaccag ccacaatgtc agcaggataa acagtacgtt
    31441 cagagccgca tacacccaga aggtgttggc gttacccagc gtgttgagca tggtcaggaa
    31501 cgttgcgcca acgatcatgt tggcaatcca gttggtggca gtggagcagg tgatgccaaa
    31561 atcgcggcct ttcagcggct gaatttcgga gcacagtacc caaatcagcg gaccggcact
    31621 catggcaaaa ccgacaataa acatcagcag catggcgatg gcgaaatact gcgccgacgg
    31681 agagtgaata ccgatatgca tcattgtacc gagtacgccc atgccagcag ccatcaccag
    31741 gaagcccagc gttagcgttg gtttacgtcc ccagcggtca acaaggccga ttgcgataaa
    31801 ggtggcaagt acgttggtca ggccgacaat cacggtcccc cacatttgct cggtagtgtt
    31861 ggtataaccc gccagttcga agattttcgg cgcgtaatac atgatgacgt tcatcccggt
    31921 gaattgctgc attacctgca acagtacgcc aaggaacacc gcgcggcgga agttgctgtt
    31981 ctctttaaac agcgcccagc cactctgttt aacctgcaaa ctttcacgga tttcatccag
    32041 ttcgcgtttc gcttccgcgc tggtgtcacg caggcgtagc agcacgcgtt cggcatcaac
    32101 aaaacggcgt ttggcggcaa accaacgtgg gctgtctggc aggaagaaga caccaatcag
    32161 cagcaaaatt gccgggatga taatcacacc cagcatccag cgccatgcac cggtgtagct
    32221 gaaggcggta tcagaaagat aagcaccgag gatcccgata gtgatcatca actgatacat
    32281 cgagatcata ctgccacgaa ttttttccgg cgcaatttca gagaggtaca gcggtgcggt
    32341 ataagaggcc acacccaccg ccagccccag tagaacgcgg gaaagaatca gtacttcaac
    32401 gtttggcgca gccgcagaga acagcgaacc ggcaacaaac aaaattgcgc cgatcatcag
    32461 gctctttttg cgcccgagtt taaaggagag ccagccgctg cccaccgcac cgactgccgc
    32521 accgaacatc atggagctta cgacccattc ttgcgtgtgc gaagtaatct ggaattcatc
    32581 tgcaataaac ggcagtgcgc cagcaattac accgatatcc aggccaaaga gtaatcccgc
    32641 cagagcggca aggaagcaga cgaaaaacgt cattgccttg tttgaccgcc cctgtttttt
    32701 agcgtcaggc atgatgccct ccaatatggt tattttttat tgtgaattaa gataggtgag
    32761 tacgacgtaa aaagatgtga agcaaatcac atcagtgtaa tcgcttacac taacatttat
    32821 cttagttatg atttaagtgc atgaaaataa ttgagttata tcatcataga ttaaacgctg
    32881 ttatctgcaa ttaagacttt actgaaaaga aatgtaacaa ctgtgaaaac cgaataataa
    32941 tatgaaaata ttacaaatct ttgtctgttg tggttggtgt aatcggtttc agttgtgatg
    33001 ataatctgcg gtgaaacgtg atcaggaaga atgatgttat tgtgcgggcg gtttttttgc
    33061 cggatgcggc gtgaacgcct atccaaaaga aaaaggccag cgcgaggctg gcctttgaac
    33121 gcaggtgaag aaagattact tcagaccggc agcatcgcgc agcagctgcg ctttgtcggt
    33181 tttttcccac gggaaatgtt cacgaccaaa gtgaccgtat gctgcggttt ctttgtagat
    33241 cgggtgcagc agatccagca tctgaatcag accgtatggg cgcaggtcga agaactcacg
    33301 taccagcagg gtcagttgtt cagaaggcac tttctcagta ccgaaagttt ctaccatgat
    33361 ggaggtcggt tcagccacgc cgattgcgta ggaaacctga atttcacaac gatcggccag
    33421 gccagcagca acgatgtttt tcgcgacata acgtgctgcg taggctgcgg aacggtccac
    33481 ttttgatgga tctttaccag agaatgcacc gccaccgtga cgcgccatgc cgccgtaggt
    33541 atcaacgata attttacgac cagtcagacc gcagtcaccc attgggccac cgataacgaa
    33601 acgaccggtc gggttgatga agaatttggt ggcagaagtc agccattcag cgggcagaat
    33661 tggcttgatg atctcttcca ttaccgcttc ttgcagcgat ttctggtcga tctcttcaga
    33721 gtgctgagtg gaaagcacga cagcatcgat accaacgatt ttgccgtcgt catactgaaa
    33781 agtcacctgg cttttcgcgt ccgggcgcag ccacggcaga gtgccgtttt tacgcacttc
    33841 agcctgacgc tgtaccagac ggtgtgcata ggtgataggt gctggcatca gcacgtcggt
    33901 ttcattagtt gcgtagccaa acatcagacc ctggtcaccc gcgccctgtt ccagcggatc
    33961 ggcacggtca acgccctggt tgatgtcagg agactgtttg ccgatagcgc tcagaaccgc
    34021 acaggagtta gcgtcaaagc ccatgtcgga atgcacatag ccaatttcgc gaacggtgtt
    34081 acgggtgatc tcttcgatgt ctacccaggc gctggtggtg atttcgccgc caactaaaac
    34141 catgccggtt tttacgtagg tttcgcaagc aacgcgtgct ttcggatcct gttcgaggat
    34201 cgcgtctaaa acggcatcag aaatttggtc agcaattttg tcaggatgcc cttcagagac
    34261 ggactcggac gtaaaaaggt gttttgccat atttaatatc acctaaagag aatttggtta
    34321 gctcaaactg ttgtgtggat tttctgtggt agcggatcct accacgactc tgcaggttaa
    34381 aaacactggc agtctgagtg ttaatcggta tggatggatt aacatctgga tggctatttt
    34441 aggtcaattc ttcaccctat ttccactttt ttttgaatcg tgtctcattc tgttaaaaac
    34501 gtggctggaa atttttcctg acaatgccgg cattctgcgt atttatcttt tgcaattttc
    34561 tgccattgtg gggtataaaa cgcggcgcgc ggcttaaata aaaagcacac gacgtttctt
    34621 tcgtgttgcc acttccagcc gggttgaaat cagagttttg gcttgtgggt tcgtcttaac
    34681 aggcggccgt ggaggtgata cgaaataatg aaccgttgtc tgctgcttaa cctgtctcac
    34741 cgttctggtg aagattcgtt ccccgcactc tgcatctctg ctttgcatac ctgccgatgt
    34801 tatacccatc tcggcgcttc tcaggattca agagctggtt acagttactg aggactgaac
    34861 aagggcgctc ttgtaaaaac aagagttttc tcgtggtttc gccgaacttt cacacttacg
    34921 ttcggttatg tgcttaataa tgttatgaaa aagaaaccgg ttgcgcagtt ggagcgtcag
    34981 cattcactgc tggaaaatcc atgtgcttat gggttgttat cgcagttcca ggctgcgata
    35041 gtcgttaact gttttacact taataaaata atttgaggtt cgctatgtct gacgacatgt
    35101 ctatgggttt gccttcgtca gcgggcgaac acggtgtact acgctccatg caggaggttg
    35161 caatgagctc ccaggaagcc agcaagatgc tgcgtactta caatattgcc tggtggggca
    35221 ataactacta tgacgttaac gagctgggcc acattagcgt gtgcccggac ccggacgtcc
    35281 cggaagctcg cgtcgatctc gcgcagttag tgaaaactcg tgaagcacag ggccagcgtc
    35341 tgcctgcact gttctgtttc ccacagatcc tgcagcaccg tttgcgttcc attaacgccg
    35401 cgttcaaacg tgcgagggaa tcctacggct ataacggcga ttacttcctt gtttatccga
    35461 tcaaagttaa ccagcaccgc cgcgtgattg agtccctgat tcattcgggc gaaccgctgg
    35521 gtctggaagc cggttccaaa gccgagttga tggcagtact ggcacatgct ggcatgaccc
    35581 gtagcgtcat cgtctgcaac ggttataaag accgcgaata tatccgcctg gcattaattg
    35641 gcgagaagat ggggcacaag gtctatctgg tcattgagaa gatgtcagaa atcgccattg
    35701 tgctggatga agcagaacgt ctgaatgtcg ttcctcgtct gggcgtgcgt gcacgtctgg
    35761 cttcgcaggg ttcgggtaaa tggcagtcct ccggcgggga aaaatcgaag ttcggcctgg
    35821 ctgcgactca ggtactgcaa ctggttgaaa ccctgcgtga agccgggcgt ctcgacagcc
    35881 tgcaactact gcacttccac ctcggttcgc agatggcgaa tattcgcgat atcgcgacag
    35941 gcgttcgtga atccgcgcgt ttctatgtgg aactgcacaa gctgggcgtc aatattcagt
    36001 gcttcgacgt cggcggcggt ctgggcgtgg attatgaagg tactcgttcg cagtccgact
    36061 gttcggtgaa ctacggcctc aatgaatacg ccaacaacat tatctgggcg attggcgatg
    36121 cgtgtgaaga aaacggtctg ccgcatccga cggtaatcac cgaatcgggt cgtgcggtga
    36181 ctgcgcatca caccgtgctg gtgtctaata tcatcggcgt ggaacgtaac gaatacacgg
    36241 tgccgaccgc gcctgcagaa gatgcgccgc gcgcgctgca aagcatgtgg gaaacctggc
    36301 aggagatgca cgaaccggga actcgccgtt ctctgcgtga atggttacac gacagtcaga
    36361 tggatctgca cgacattcat atcggctact cttccggcat ctttagcctg caagaacgtg
    36421 catgggctga gcagctttat ttgagcatgt gccatgaagt gcaaaagcag ctggatccgc
    36481 aaaaccgtgc tcatcgtccg attatcgacg agctgcagga acgtatggcg gacaaaatgt
    36541 acgtcaactt ctcgctgttc cagtcgatgc cggacgcatg ggggatcgac cagttgttcc
    36601 cggttctgcc gctggaaggg ctggatcaag tgccggaacg tcgcgctgtg ctgctggata
    36661 ttacctgtga ctctgacggt gctatcgacc actatattga tggtgacggt attgccacga
    36721 caatgccaat gccggagtac gatccagaga atccgccgat gctcggtttc tttatggtcg
    36781 gcgcatatca ggagatcctc ggcaacatgc acaacctgtt cggtgatacc gaagcggttg
    36841 acgtgttcgt cttccctgac ggtagcgtag aagtagaact gtctgacgaa ggcgataccg
    36901 tggcggacat gctgcaatat gtacagctcg atccgaaaac gctgttaacc cagttccgcg
    36961 atcaagtgaa gaaaaccgat cttgatgctg aactgcaaca acagttcctt gaagagttcg
    37021 aggcaggttt gtacggttat acttatcttg aagatgagta agtcctgtgt tacttgaatc
    37081 cgcttaattt agcggtgata atccgccaca atttattgtg acaaatccaa cccttcctcg
    37141 tcgggcctaa cgacgcggaa gggttttttt atatcgactt tgtaatagga gtccatccat
    37201 gagcacctta ggtcatcaat acgataactc actggtttcc aatgcctttg gttttttacg
    37261 cctgccgatg aacttccagc cgtatgacag cgatgcagac tgggtgatta ctggcgtgcc
    37321 gttcgatatg gccacttctg gtcgtgcggg tggtcgccac ggtccggcag cgatccgtca
    37381 ggtttcgacg aatctggcct gggaacacaa ccgcttcccg tggaatttcg acatgcgtga
    37441 gcgtctgaac gtcgtggact gcggcgatct ggtatatgcc tttggcgatg cccgtgagat
    37501 gagcgaaaag ctgcaggcgc acgccgagaa gctgctggct gccggtaagc gtatgctctc
    37561 tttcggtggt gaccactttg ttacgctgcc gctgctgcgt gctcatgcga agcatttcgg
    37621 caaaatggcg ctggtacact ttgacgccca caccgatacc tatgcgaacg gttgtgaatt
    37681 tgaccacggc actatgttct ataccgcgcc gaaagaaggt ctgatcgacc cgaatcattc
    37741 cgtgcagatt ggtattcgta ccgagtttga taaagacaac ggctttaccg tgctggacgc
    37801 ctgccaggtg aacgatcgca gcgtggatga cgttatcgcc caagtgaaac agattgtggg
    37861 tgatatgccg gtttacctga cttttgatat cgactgcctg gatcctgctt ttgcaccagg
    37921 caccggtacg ccagtgattg gcggcctgac ctccgatcgc gctattaaac tggtacgcgg
    37981 cctgaaagat ctcaacattg ttgggatgga cgtagtggaa gtggctccgg catacgatca
    38041 gtcggaaatc actgctctgg cagcggcaac gctggcgctg gaaatgctgt atattcaggc
    38101 ggcgaaaaag ggcgagtaag caccagatgc gatgcgcacg ggtaaaacgt gccattaatg
    38161 tcggatgcgg cgtgaacgcc ttatccgacc tacgttcggc acccgtaggc cggataagat
    38221 gcgccagcat cgcatccggc aatgcgcaca aggtaacaaa tgtgccattc atgtcagatg
    38281 cggcgtgaac gccttatctg acctacgttc gacaccacca ggctttactt aatcccatcc
    38341 gcgctcatgc gatcgcgaat atgctgggcg cgttcggcgg atgcaggatg gtcgtcaaac
    38401 attgagcttt ggcgaccttc ttccagtttt gccagttttt caaagctggt ggcaagacct
    38461 gccgggctga tgccgcgttg gcgcagaaga tcgtaagaat aatcatcggc ttctgcttcc
    38521 tggcgctggg agaattgcga attgactaat ttctcgccca gattaccaag ttgtgattga
    38581 gataaacttc cgacaatccc gcccgcagag gccgcagcta ctcgcacggc atttgtacca
    38641 agtgccacct gcattccttt tttcacatgg cctaacgcca cgtgccccat ttcgtgaccg
    38701 atcaccgctt cgacttcatt atccgtcatc atatccatca gcccgctata gacgcggata
    38761 cagccgttag ccattgcaaa ggcgttcaca tccttcgcca tatacacttt gtaatttacc
    38821 ggctgaccgt tgatattgtt gcctagcgca ttggcaatag ttgtcagacg tttagcgtat
    38881 tcgctattgg ctggcgcaat cgtcgccttg ctgtccatct cctgacatgc ctgatcgctc
    38941 agggttttca cctgcgcatc actcaaactg taagcctgaa aagcttccgc tcctgatgag
    39001 agcagtccgt tggagtccat attctggcaa ccagtcagta ccgttgccac gctcattgct
    39061 accaataagg cgcgaatttt catgttttct cttccgcaca aggtcaggat tattctaaaa
    39121 atgcgccgtt tgcaggtgaa tcgacgctca gtctcagtat aaggaaaagc gcagcggacg
    39181 ggcgagtaga ttgcgcaaca tgcgagcatg atccagagat ttctgaagcg gcaaaaggat
    39241 gttccatgta catgacgcgc ggcttgcggt aaattgttgg caaattttcc ggcgtagccc
    39301 aaaacgcgct gtcgtcaagt cgttaagggc gtgcccttca tcatccgatc tggagtcaaa
    39361 atgtcctcac gtaaagagct tgccaatgct attcgtgcgc tgagcatgga cgcagtacag
    39421 aaagccaaat ccggtcaccc gggtgcccct atgggtatgg ctgacattgc cgaagtcctg
    39481 tggcgtgatt tcctgaaaca caacccgcag aatccgtcct gggctgaccg tgaccgcttc
    39541 gtgctgtcca acggccacgg ctccatgctg atctacagcc tgctgcacct caccggttac
    39601 gatctgccga tggaagaact gaaaaacttc cgtcagctgc actctaaaac tccgggtcac
    39661 ccggaagtgg gttacaccgc tggtgtggaa accaccaccg gtccgctggg tcagggtatt
    39721 gccaacgcag tcggtatggc gattgcagaa aaaacgctgg cggcgcagtt taaccgtccg
    39781 ggccacgaca ttgtcgacca ctacacctac gccttcatgg gcgacggctg catgatggaa
    39841 ggcatctccc acgaagtttg ctctctggcg ggtacgctga agctgggtaa actgattgca
    39901 ttctacgatg acaacggtat ttctatcgat ggtcacgttg aaggctggtt caccgacgac
    39961 accgcaatgc gtttcgaagc ttacggctgg cacgttattc gcgacatcga cggtcatgac
    40021 gcggcatcta tcaaacgcgc agtagaagaa gcgcgcgcag tgactgacaa accttccctg
    40081 ctgatgtgca aaaccatcat cggtttcggt tccccgaaca aagccggtac ccacgactcc
    40141 cacggtgcgc cgctgggcga cgctgaaatt gccctgaccc gcgaacaact gggctggaaa
    40201 tatgcgccgt tcgaaatccc gtctgaaatc tatgctcagt gggatgcgaa agaagcaggc
    40261 caggcgaaag aatccgcatg gaacgagaaa ttcgctgctt acgcgaaagc ttatccgcag
    40321 gaagccgctg aatttacccg ccgtatgaaa ggcgaaatgc cgtctgactt cgacgctaaa
    40381 gcgaaagagt tcatcgctaa actgcaggct aatccggcga aaatcgccag ccgtaaagcg
    40441 tctcagaatg ctatcgaagc gttcggtccg ctgttgccgg aattcctcgg cggttctgct
    40501 gacctggcgc cgtctaacct gaccctgtgg tctggttcta aagcaatcaa cgaagatgct
    40561 gcgggtaact acatccacta cggtgttcgc gagttcggta tgaccgcgat tgctaacggt
    40621 atctccctgc acggtggctt cctgccgtac acctccacct tcctgatgtt cgtggaatac
    40681 gcacgtaacg ccgtacgtat ggctgcgctg atgaaacagc gtcaggtgat ggtttacacc
    40741 cacgactcca tcggtctggg cgaagacggc ccgactcacc agccggttga gcaggtcgct
    40801 tctctgcgcg taaccccgaa catgtctaca tggcgtccgt gtgaccaggt tgaatccgcg
    40861 gtcgcgtgga aatacggtgt tgagcgtcag gacggcccga ccgcactgat cctctcccgt
    40921 cagaacctgg cgcagcagga acgaactgaa gagcaactgg caaacatcgc gcgcggtggt
    40981 tatgtgctga aagactgcgc cggtcagccg gaactgattt tcatcgctac cggttcagaa
    41041 gttgaactgg ctgttgctgc ctacgaaaaa ctgactgccg aaggcgtgaa agcgcgcgtg
    41101 gtgtccatgc cgtctaccga cgcatttgac aagcaggatg ctgcttaccg tgaatccgta
    41161 ctgccgaaag cggttactgc acgcgttgct gtagaagcgg gtattgctga ctactggtac
    41221 aagtatgttg gcctgaacgg tgctatcgtc ggtatgacca ccttcggtga atctgctccg
    41281 gcagagctgc tgtttgaaga gttcggcttc actgttgata acgttgttgc gaaagcaaaa
    41341 gaactgctgt aattagcatt tcgggtaaaa aggtcgcttc ggcgaccttt tttattacct
    41401 tgatatgtcc gtttgcggac aagcaataga taaggcgtgt tgtagatcac aaatatttat
    41461 atgcaataaa tatcaattat gtaata
//
LOCUS       DBBGLQ010000035        41254 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.035, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000035 DBBGLQ010000000
VERSION     DBBGLQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.035"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..953
                     /locus_tag="ACIYSB_002496"
     CDS             <1..953
                     /locus_tag="ACIYSB_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429530.1"
                     /translation="NTQNALPKNYIIGTPLILPTSKNESIEKIHIFFMWKDILSLIAD
                     HHNSDCSVLFFTNEDKSYTVELTHFLQYSEINRLSNSSLKYEIIKELLDTIKINDLHK
                     SERKLVIRSAINEVFKANGTFNFFDLLNSTELVRKKYDELYEIYTKRFSVNKILNELD
                     EKNLEFTSKINEFISSNQTKALTIPGALIAAGGLVKANETTEAILIIAGLWMIKKVNY
                     ISIEIFNETFDNLRSRVESAFDKYLKFEENKEIKDNADSIKSSITGLIDKAKKRMRTV
                     KYLASAMFYGGLIYVGYKQFPVFFEKSAVNLFYFLCHTIS"
     gene            complement(933..1289)
                     /locus_tag="ACIYSB_002497"
     CDS             complement(933..1289)
                     /locus_tag="ACIYSB_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGC5429531.1"
                     /translation="MRDMYEVMDRWGAWAASDNSGVDWQPIAAGFKGLLPHGKKSRIQ
                     CDDDEGIMIDSCVARLRRYKPEEYELIIAHFVIGISLRTIAKKRKCSDGTIRKELQTA
                     MGFVDGCLAMLAYSMA"
     gene            complement(1307..2296)
                     /locus_tag="ACIYSB_002498"
     CDS             complement(1307..2296)
                     /locus_tag="ACIYSB_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGC5429532.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQTLADDPAVRAVFRNETVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVEPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2304..3113)
                     /locus_tag="ACIYSB_002499"
     CDS             complement(2304..3113)
                     /locus_tag="ACIYSB_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGC5429533.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(3133..3522)
                     /locus_tag="ACIYSB_002500"
     CDS             complement(3133..3522)
                     /locus_tag="ACIYSB_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGC5429534.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(3519..3845)
                     /locus_tag="ACIYSB_002501"
     CDS             complement(3519..3845)
                     /locus_tag="ACIYSB_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGC5429535.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            complement(3842..4495)
                     /locus_tag="ACIYSB_002502"
     CDS             complement(3842..4495)
                     /locus_tag="ACIYSB_002502"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGC5429536.1"
                     /translation="MSNKYCQALAELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLVELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(4495..4989)
                     /locus_tag="ACIYSB_002503"
     CDS             complement(4495..4989)
                     /locus_tag="ACIYSB_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGC5429537.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQNVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQDPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLERRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(4986..5927)
                     /locus_tag="ACIYSB_002504"
     CDS             complement(4986..5927)
                     /locus_tag="ACIYSB_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5429538.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSCPDASQP
                     DTQKAEQDFLTRYPDAVVFSPKNRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(5917..6096)
                     /locus_tag="ACIYSB_002505"
     CDS             complement(5917..6096)
                     /locus_tag="ACIYSB_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGC5429539.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(6272..6829)
                     /gene="ymfL"
                     /locus_tag="ACIYSB_002506"
     CDS             complement(6272..6829)
                     /gene="ymfL"
                     /locus_tag="ACIYSB_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGC5429540.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSTNGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(6873..7073)
                     /gene="ymfT"
                     /locus_tag="ACIYSB_002507"
     CDS             complement(6873..7073)
                     /gene="ymfT"
                     /locus_tag="ACIYSB_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGC5429541.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            7164..7838
                     /gene="ymfK"
                     /locus_tag="ACIYSB_002508"
     CDS             7164..7838
                     /gene="ymfK"
                     /locus_tag="ACIYSB_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HGC5429542.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            8073..8279
                     /locus_tag="ACIYSB_002509"
     CDS             8073..8279
                     /locus_tag="ACIYSB_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429543.1"
                     /translation="MHKTEPKIVAPGYTNEEIYEWMAKKLAAINQLREVLSYRQVTID
                     SLKKLDQEITVLSQDVTLDIVQTN"
     gene            complement(8251..8685)
                     /locus_tag="ACIYSB_002510"
     CDS             complement(8251..8685)
                     /locus_tag="ACIYSB_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429544.1"
                     /translation="MSGNIYTLYKSHCENVGKYRGIEISGVVSSVEISKVESRATLLT
                     LLDLVLHEHRKKFGTPYNQLNGKKALVHLILMKHHWMPKQINEMKFDELLLSIQDELT
                     LDKISVTAQKFLDYRDWRSQIHHFDDFDENEWDPNLSAQYLK"
     gene            8829..9142
                     /locus_tag="ACIYSB_002511"
                     /pseudo
     CDS             8829..9142
                     /locus_tag="ACIYSB_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9143..9340
                     /locus_tag="ACIYSB_002512"
                     /pseudo
     CDS             9143..9340
                     /locus_tag="ACIYSB_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            9321..10448
                     /gene="intE"
                     /locus_tag="ACIYSB_002513"
     CDS             9321..10448
                     /gene="intE"
                     /locus_tag="ACIYSB_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGC5429545.1"
                     /translation="MAARPRKNNVSVPNLYPLYSRKVNKVYWRYKHPITGKFHALGTN
                     EAEAIAIATEANTRLAEQRTRQILAISDRIATSKGKAITTSTWLDRYQAIQNDRLKSG
                     DIKLNTYKQKAKPVSLLRERAGLKLISAVDVRDIAQLLDEYIAAGQPRMAQVVRSVLI
                     DVFKEAQHYGEVPPGYNPALATKQPRRKITRQRLSLEEWKKIFDIADASHRYMGNAML
                     LALVTGQRLGDISRMKFSDIWDDHLHVVQEKTGSKIAIPLSLRLNAINWSLRDVVARC
                     RDYAVSPYLVHFFRSTSQAERGAQVKSNTLTMNFSKARDLAEINWGEGSPATFHEQRS
                     LSERLYKQQGLDTQKLLGHKTQQQTDRYHDDRGKGWSKVAL"
     gene            complement(10662..11591)
                     /locus_tag="ACIYSB_002514"
     CDS             complement(10662..11591)
                     /locus_tag="ACIYSB_002514"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429546.1"
                     /translation="MSEITSAPAVMAALVSALVTVLLFFIKGVCTPLWNKYFIVYKIK
                     VEHSYEQKKKIKEAISKYKMPLLDSAESLNHRLWNFSGNCTKGWHNFKNNESIGDKYY
                     LQTFCYRFLAFYAWCIKFERELIYLDVTLSEKNDLYFVKYIKTMQNIFCDASMLSGTG
                     YDNEHAVDHFFKDDLISIVEKMITPTGVITYSEFKSKSESELDYTQVCKYISTIMSDK
                     SCNKWHLINCFHFILMAFLSKYGYDYQRTGLFKLYKLRCSEPQNKLISNFERLVFNAR
                     LNKCREIKKAITILECKSNIANLCNSLKLIKEN"
     gene            11973..13310
                     /locus_tag="ACIYSB_002515"
     CDS             11973..13310
                     /locus_tag="ACIYSB_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN/Toprim-associated domain-containing
                     protein"
                     /protein_id="HGC5429547.1"
                     /translation="MGTVISLEVSGMGLDWSKNSLGIDHGGLFQSADHHVKPLNEDQI
                     EEGEDFDTPDGILCRTVLRKPLGQVAYRLELLGFTLENIRYEYELMAKDSIEYQEEFN
                     ESCPEYAKPISNMMSFDEFVNFIKSVNISELNDDYISLKDRIKERELIMGRFNNDELL
                     SRIPQYDNAFDNAWSEKSAFGTLVSILHPYSVMRLLAENPNNHNEFVTWDYGDLVSAG
                     YANIKDIQVNARRRDKFLIATEGSTDSNVIKYALHLLRPDVEDFFHFIDMSEGYPFTG
                     SGNLVKFAAGLVKIDIQNLIVFVLDNDCEGIEAYEKIRKLALPHNISCIHLPNLPHFN
                     QFPAIGPHGVTYENINGRAVAIECYLDLRFSDYDEAKIRWSNYKKESDAYQGALENKD
                     YYTKKFMKLKPGSKGFSNYDFAKLAIVLDELIKACSKISSKIRKNEVDVQYPD"
     gene            complement(13338..13517)
                     /locus_tag="ACIYSB_002516"
     CDS             complement(13338..13517)
                     /locus_tag="ACIYSB_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429548.1"
                     /translation="MNVNTEILKLWQSRGENERTGFDAERVADIAWEQGLQLHRNRQE
                     HYQRVMYLIGHRNIG"
     gene            complement(13917..15167)
                     /gene="icd"
                     /locus_tag="ACIYSB_002517"
     CDS             complement(13917..15167)
                     /gene="icd"
                     /locus_tag="ACIYSB_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGC5429549.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            15339..15992
                     /gene="rluE"
                     /locus_tag="ACIYSB_002518"
     CDS             15339..15992
                     /gene="rluE"
                     /locus_tag="ACIYSB_002518"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGC5429550.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            16002..16463
                     /gene="nudJ"
                     /locus_tag="ACIYSB_002519"
     CDS             16002..16463
                     /gene="nudJ"
                     /locus_tag="ACIYSB_002519"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGC5429551.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            16517..17623
                     /gene="mnmA"
                     /locus_tag="ACIYSB_002520"
                     /gene_synonym="trmU"
     CDS             16517..17623
                     /gene="mnmA"
                     /locus_tag="ACIYSB_002520"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGC5429552.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            17659..18300
                     /gene="hflD"
                     /locus_tag="ACIYSB_002521"
     CDS             17659..18300
                     /gene="hflD"
                     /locus_tag="ACIYSB_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGC5429553.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            18304..19674
                     /gene="purB"
                     /locus_tag="ACIYSB_002522"
     CDS             18304..19674
                     /gene="purB"
                     /locus_tag="ACIYSB_002522"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGC5429554.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            19843..20514
                     /gene="phoP"
                     /locus_tag="ACIYSB_002523"
     CDS             19843..20514
                     /gene="phoP"
                     /locus_tag="ACIYSB_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGC5429555.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            20514..21974
                     /gene="phoQ"
                     /locus_tag="ACIYSB_002524"
     CDS             20514..21974
                     /gene="phoQ"
                     /locus_tag="ACIYSB_002524"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGC5429556.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            22050..23171
                     /gene="roxA"
                     /locus_tag="ACIYSB_002525"
                     /gene_synonym="ycfD"
     CDS             22050..23171
                     /gene="roxA"
                     /locus_tag="ACIYSB_002525"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGC5429557.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23220..24446)
                     /gene="pepT"
                     /locus_tag="ACIYSB_002526"
     CDS             complement(23220..24446)
                     /gene="pepT"
                     /locus_tag="ACIYSB_002526"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGC5429558.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKKI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            24696..25832
                     /gene="potA"
                     /locus_tag="ACIYSB_002527"
     CDS             24696..25832
                     /gene="potA"
                     /locus_tag="ACIYSB_002527"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGC5429559.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            25816..26673
                     /gene="potB"
                     /locus_tag="ACIYSB_002528"
     CDS             25816..26673
                     /gene="potB"
                     /locus_tag="ACIYSB_002528"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGC5429560.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            26670..27464
                     /gene="potC"
                     /locus_tag="ACIYSB_002529"
     CDS             26670..27464
                     /gene="potC"
                     /locus_tag="ACIYSB_002529"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGC5429561.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27461..28507
                     /gene="potD"
                     /locus_tag="ACIYSB_002530"
     CDS             27461..28507
                     /gene="potD"
                     /locus_tag="ACIYSB_002530"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGC5429562.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            28565..29026
                     /gene="ymfA"
                     /locus_tag="ACIYSB_002531"
     CDS             28565..29026
                     /gene="ymfA"
                     /locus_tag="ACIYSB_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HGC5429563.1"
                     /translation="MSQDSKVFFRIFLGIGLVLILISVVVFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKDGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKSRRKRKSRP"
     gene            29023..29811
                     /gene="ycfZ"
                     /locus_tag="ACIYSB_002532"
     CDS             29023..29811
                     /gene="ycfZ"
                     /locus_tag="ACIYSB_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HGC5429564.1"
                     /translation="MKKFIILLSLLILLPLTAASKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGIAPFIVVLPDINNEASLRQNGKAMLAHASSSLSDVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLHAGLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGKQHENDPPGSTNTLGFIAWAATFVLFSSVFYYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAVLLLTSKR"
     gene            complement(29930..30769)
                     /gene="cobB"
                     /locus_tag="ACIYSB_002533"
     CDS             complement(29930..30769)
                     /gene="cobB"
                     /locus_tag="ACIYSB_002533"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415638.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGC5429565.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGLKAG
                     SIA"
     gene            complement(30785..31696)
                     /gene="nagK"
                     /locus_tag="ACIYSB_002534"
     CDS             complement(30785..31696)
                     /gene="nagK"
                     /locus_tag="ACIYSB_002534"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGC5429566.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITAQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31725..32969)
                     /gene="lolE"
                     /locus_tag="ACIYSB_002535"
     CDS             complement(31725..32969)
                     /gene="lolE"
                     /locus_tag="ACIYSB_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGC5429567.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32969..33670)
                     /gene="lolD"
                     /locus_tag="ACIYSB_002536"
     CDS             complement(32969..33670)
                     /gene="lolD"
                     /locus_tag="ACIYSB_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGC5429568.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33663..34862)
                     /gene="lolC"
                     /locus_tag="ACIYSB_002537"
     CDS             complement(33663..34862)
                     /gene="lolC"
                     /locus_tag="ACIYSB_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGC5429569.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            35124..36197
                     /gene="ycfT"
                     /locus_tag="ACIYSB_002538"
     CDS             35124..36197
                     /gene="ycfT"
                     /locus_tag="ACIYSB_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGC5429570.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            36325..39771
                     /gene="mfd"
                     /locus_tag="ACIYSB_002539"
     CDS             36325..39771
                     /gene="mfd"
                     /locus_tag="ACIYSB_002539"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGC5429571.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSMVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39918..40877
                     /gene="ldtC"
                     /locus_tag="ACIYSB_002540"
     CDS             39918..40877
                     /gene="ldtC"
                     /locus_tag="ACIYSB_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGC5429572.1"
                     /translation="MIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGENK
                     FHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPREG
                     IVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTANI
                     RARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCIR
                     LRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPIT
                     LNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40959..41216)
                     /gene="bhsA"
                     /locus_tag="ACIYSB_002541"
                     /gene_synonym="ycfR"
     CDS             complement(40959..41216)
                     /gene="bhsA"
                     /locus_tag="ACIYSB_002541"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGC5429573.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT        10496 a        10458 c         9921 g        10379 t
ORIGIN      
        1 taaacaccca aaacgctctt ccgaaaaatt atatcatcgg aacgccgtta atactcccta
       61 cttctaagaa tgaaagcatc gaaaaaatcc atattttctt tatgtggaaa gatatccttt
      121 cattaattgc tgatcatcat aacagtgact gctctgtctt atttttcacc aatgaagaca
      181 aaagttatac agtcgaactc acgcattttt tacaatatag cgagattaat cgcttatcga
      241 actcgtctct taaatatgaa attataaagg agcttctcga cacaataaaa atcaatgatt
      301 tacacaaaag cgagcgcaaa ctcgttatac gctcagccat aaacgaagta tttaaagcaa
      361 atggtacgtt caatttcttt gacttgctta actctactga actcgtcagg aaaaaatatg
      421 atgaactata tgagatttac acaaagaggt tttctgtaaa taaaattctt aacgaactcg
      481 atgagaaaaa tcttgagttc acgagtaaaa ttaacgaatt catatcatct aatcagacaa
      541 aagcattgac tattcccggt gcattaatag cagctggtgg cttagtgaag gctaatgaaa
      601 caaccgaagc aatattgatt atcgcaggac tttggatgat aaaaaaagtc aattacattt
      661 ctattgagat attcaatgaa acattcgaca acttacgttc tcgagtggag tccgctttcg
      721 ataagtattt aaagtttgaa gaaaataaag aaatcaaaga taatgcagat agtattaaga
      781 gtagcattac aggtttgatt gataaagcta aaaaaaggat gagaactgtt aaatatctcg
      841 catcagccat gttttatgga ggccttattt acgttggata taaacagttc ccagtctttt
      901 ttgaaaaatc ggcagtaaat ctattttatt ttttatgcca tactataagc taacattgct
      961 aaacagccgt caacaaaccc cattgcagtt tgcagttcct tcctaattgt gccatcagag
     1021 cattttctct tcttcgcaat agtgcgtaat gagataccga taacaaagtg ggcgatgatg
     1081 agctcatatt cctctggttt ataccttctc aaccgagcca cacaactgtc tatcatgatg
     1141 ccttcgtcat catcacactg aatccgtgac tttttgccat gaggtaaaag tcccttgaaa
     1201 ccagccgcta tcggctgcca gtccactccg ctattgtctg aagcagccca agctccccaa
     1261 cgatccatta cttcatacat atcacgcatc aactttctcc acaaaatcag gccagcacac
     1321 caattgccag cgcacgatcg ataaaacgaa atatcaactc cagctgggag ccatacttct
     1381 cttcaaatgc cacggtatcc gcatgcagct cgtcgtgatg ctttctgcac aaaggcaaca
     1441 caaagagatc atgcgctttt gtacccattc ccccctgacc gtggcctatc agatggtggg
     1501 gatcatcagc aggctttcca caacatgcac acggctgcgt cttaacccag cgcgtgtact
     1561 tttcattaac ccagcggcga cgttttgggc gtaacataaa agactccggc gactccggct
     1621 ccactttcag cgccagcacc tttttcgctt tatcctggat gatgctggtg gcaggaaccg
     1681 aaggaacaag gtcactttcc cgggtgacag acggcacaac aggcttcggt aatctcagtg
     1741 ccttacgggc tgcactttcc ggtaaggcat ccgccaggtc attacgaacc agccaccagc
     1801 atagttccgg cattgtcaca acgtgactat catcaaaacc gagatcccga cgcacaacag
     1861 acaataccca gcgggcacag ttatccgttg ccattgattc cagccgttcc gtgaactgat
     1921 cgcgcagctg gttatcgcag tgccagcaca gacggattgc gcccggagcg tgtcgcattg
     1981 tggtcatgtt ctcgctgtgc cagtcggaat gaggccactg gcagcctttt tcacgaagta
     2041 accagctttc aagacattcc accccaccag cacgacggat cactgtctca ttgcggaaca
     2101 cggcccgaac ggcaggatca tccgccagcg tttgtgatgc cgccggaacg gcaccactgg
     2161 caaaagatga ataacgttcc ggctcaggct ccagcaggac acgcccctgc ataaacaggg
     2221 gcatcagctc tgaacctggt ctgaacaata cgatccccat acgcggggca atttcagggg
     2281 tcagtagcgc tctcacggtc acctcaatga acggtatcga gcagctttaa cagctcaggg
     2341 aatcgggatt cgaagaaatg cggctgcgtc tcgcgcggat ttgcgggact ggtgatgttc
     2401 ttgccgaaca tgcaaccttt cgctgtcagc gaccagaatt ttttgatgtt gttaatcgcg
     2461 gtacggctgt atcgttcgcg ctgctcgacg atccccagtt tcaccatctg gtgatatgcc
     2521 tgattagccg tcaggcgtat accatactgt ttcagcagtg cgctcagtga cagtgtcggg
     2581 cgacttgagc catcgtgtgc atcagcagga gcatcaatgg catagcgcgg tgccagattc
     2641 ggtaagccaa cagcctcctg gagtttctga caggcaccaa gcactgaaga gttagacagg
     2701 tttaactccc ggcgcataaa gtccagcagg atcacgccag cctgcatctt gtcagcagcc
     2761 tgtccggata atttttccgg tgcgctggtt accatatcga aagtacggat caccttcaga
     2821 tggaatgacg ggctgatcca cattgcatag gcatacacca gttccttgca gacatacgtt
     2881 ccccgttcat ttcccccatg aatcacactc accgggtcaa cacccaaatt ctgggtgttg
     2941 gtcaattcat gaacaagctc aacagtttgt tggctggaaa gaaactttcc cggctccttg
     3001 gttctggcat ttgcaccaga tgctactgct gcgcgatgca gatcgttcag gctgtaacgc
     3061 ccataagcat cacgacgaac ttcaatacca tcaatgacca tcagattatt catacttcgt
     3121 ttctcctttt gctcaggcgg ctgcacccgc cgttttctcg tacttactga tagtgatctc
     3181 gaccttccct tccgggataa ccggtcccca ctccaccagc attcttttca cctgactgtc
     3241 gtcttcccac acacccgcgt gggtcagggc gtcaaacagt gccttgttat agttgtccag
     3301 atcgcggatc cggttatccg gaggaaacaa cacgatctcc actgaagcag gtgccgacgt
     3361 tggtttcggc agacgacgta actgctcaac tattgctgcg cacgccgcgc tctggaattt
     3421 gcgccccgcc gcgcttatca ggctcttacc tgcaaacgcc cctttgttgg ggtgtcgcca
     3481 gtacgtgttc acgctgggcg gaaaaggcag gatcagcttc atactttcag gtccctctca
     3541 tgtaaccagt gggttgcacg cagccttgcg ttttcctcac cggcaagcag tgcgcggata
     3601 atcccgaccg cctcgctgtc gtcgtccttc accgcggtat gaagcgttat cccccgggcc
     3661 acgccacgct ttatcgtgat gacgcctttt ttctccagtg cgcgaagatg ctccaccgct
     3721 gcattcactg aacggtatcc cagcatggtt gccacctcct gattggttgg cgggaagcca
     3781 cgttctttct gataagaaat cagcatatcc agcacctgct gctggcattg agttaacgtc
     3841 gtcatgccgc catctccctg accagttttt ccgcctgctg gcgaacctgc gccagaaacg
     3901 cctcaccaca tgcctcaagt tcatcgcgcc cgatgtagct gattgccggt cccttccagg
     3961 tcttatcgaa aacagcaata gcaccagcga agaaagcgcc tgtcggcacc tgcttctcat
     4021 ccttcgggat aaaccaggca ggcagttcaa aaccaatacg cccgcgaata aaagcaatat
     4081 gatctgcatc ttccggccac cacacttcgc tggtggcagc tttgatcagg aaaacatagc
     4141 gcccgccctt atcacgcatg gcactggcat gcttcatgat gtaacgcatg ccagtgatgt
     4201 attgcccctc atgctgactg gcgcggctgt atgggggatt accaaaggca gcacctttaa
     4261 gctccacaag acgttctgac cagtcatgcg ccagcgcgtt gtcttccgcc gtgtaatacg
     4321 cagcacattt ggcgttatca ccatcagtga acagatccag aacaaacggg ccaaacaggg
     4381 tgttaattcc ccagaaaatg ttatccggcg tgcgccactg atcgcccact tccttcagtt
     4441 catgggctgg tttgttccgc agttccgcca gcgcctggca atatttatta ctcattaagc
     4501 ccccacgtaa ttccctgaca gataccactc ttcacccgat gccgcgcgct tgctgctttt
     4561 ccgtaagcac cgctcacgac gctccagaaa attgtttcgt tctggctggg agtggctttc
     4621 acggaatgcc gccatccaca ccgttgcagc acgacggtat aagcccctgg actccagttc
     4681 ttccgcctgg cgggtcaggc acaaaatcac ccgcgggtcg ttagtgccga catagaaatt
     4741 gcgcacaggt ctggtttcac gaactggttg tggttccgga tcctgcgctc tctcagtcag
     4801 gcgcgggaaa tgtctgcgtg tatctccttc acaacggtga gccacacgcc cactctgacg
     4861 taacttgctt gctgactgca gaacgttctg ccgtgagtaa cctgcaaaag catccgcaat
     4921 gtctccggaa gtacaccccg gatgggcttc aatgaatttc tgaacttcat tcaaaagact
     4981 cataatcacc ccctgaatcc tgcagggatc tggctgtagt ccacgttgtc gtaactggct
     5041 ttgaagtacg ggtcttcgcg tttttctgtg tacgtgctga cggacggcga taagcgcagg
     5101 gaaagctcat cccatttttc ccgcaacttc gacgggctga gcacgttacg gcaccagaac
     5161 ggatcgcggc tgacgcggct gtacatctcg cagatttgtt tgtgagtacg accatcctgc
     5221 acacacatca ggcgaatttc gtttgcccag gctgtccagt taggttcttt gggacgaacc
     5281 acctcgccgt cacattcggc ggcctgctcg tacagggcga tgattttttt ccagagccac
     5341 tgtgcgcagg tcaaatcatc ctgcgttccc cactggcgat ttttagggct gaatacaacc
     5401 gcatcaggat agcgagttaa aaaatcctgt tcagccttct gcgtgtccgg ttgcgaagcg
     5461 tccggacaag aaggtttttt atctgacgga tcatgttttg attttactga cggatccccg
     5521 ccagattctg acgggtgaaa acccgctttt ttgccagatt tcgacgcatc aaattttgac
     5581 gggtcagatt ttgatgcgtc agattttgac gggtcagaat ctgacagttg agaaaatgcc
     5641 gctgcctgaa gcttcgcaac gttaagctga taaacattcg acgcattgcg gttaccctgg
     5701 cgacgcgcct tacgcgttaa ccagccttct gcttccagcc gtgcgatagc cgttctgacg
     5761 gtactcatcc ccgcgccaat ctggcgggca atggtttcaa ttgatggcca gcacacacct
     5821 tcgtcattac tgaaatcagc caggcgggcc ataattgcca cgctggataa cttcatgcct
     5881 gacgcagcgc aaccatccca tacatagccg gttaatttag tgctcatgac cgacctctat
     5941 ttctctgaat ttacgacgaa actgttcgag cgggctgaag cactcatgct catagccttc
     6001 gcggaggtag ataacccgtt gtgtttccgg ctcccaacga atgactctga cgggtactcc
     6061 gtagtgatct ttgaaccagc ggttaacttg tcgcaaagga ctgtctcctt ctgccggttg
     6121 aaatcaccca cagcccactc tgcaaagctg tgggttacaa tttccctgtc acctggtaca
     6181 ttcactgcat agcaatattc caccttcgct tttccacccg gtacaggaag cgcaatcagt
     6241 tgcgagcgac ggtagtgtgt tgttaaactg ttcatgcgtt agtttctcca caaccagaag
     6301 caatcgacgc cacgacgccc ggagctgcac actcgcgggc gtcattactt tctgaaatgc
     6361 aaaaaatttt gtagaccagt gctgcatgct cctgcagctt cgaaattgag agatacagct
     6421 cgtcgttaat tgctgtcttc tcatgcggtt ccactacacc gtcttcgatt gctgaacgaa
     6481 tctgttttga ataactgccg atctgttcaa tgacttccag cagacgctgg ttaatatcgg
     6541 cgttgtccac atcctcgacg tcaggaagag acacaaagac gccatttgta gactgcgcca
     6601 cagcatcagc aatgaagtga gtgccaccag cacgctgtaa aaccattgcc catcccagcg
     6661 ggaaaatctg atcgccatct gcacgaaggc ggttgaataa agcgttttct gttacatcga
     6721 gccagtcagc cgcttcagcg taaccacccg gcaacgccgc gatagttttt ctgacagctt
     6781 tcacgtacca ctcaggctgt ttttctattt tccagtgatg cttacccacg attagcctca
     6841 tcgttctgtg gttaaaaatt gaaagtgttc tgctaatctt tcggatagat atccggtctt
     6901 aagtcagatt tcgtaattgc acctgacgtg cattgctcaa gttttttagc cagcacaaaa
     6961 ctggcttttt tatagccatt gaaaaccagc cgtaagtagc caggtgttga gccaactttt
     7021 ccggccaact cgccctgctg ttctttggtt aaagagtccc aatacgcttt catacgatat
     7081 gtacctacgg tgtacatatt acatgattga aatgaacctt caagatactt gtaccttaac
     7141 ggtacaaggg ttttaatttc gttatgaaaa caatccatga catccggcgg tctaacgcca
     7201 gaaaactgag agatggtgtt ggcgggaatt cttcctttgc cactatgatt gatcgcgagc
     7261 caacccagac cagcaggttt atgggagatg gtgctactaa aaatatcggt gacagcatgg
     7321 cacgacacat cgaaaaatgt ttcgacctgc ctgtcggatg gctcgatcaa gaacaccaga
     7381 caacgaacat cacaaaaaaa cctgatgttt caatcactaa taaacaaatc acattagtcc
     7441 ctgtcatatc atgggtacag gccggagcat ggaaagaagt tggatattct gaggttgatt
     7501 tgagcacagc agaaacgtat ccctgccctg taccctgtgg ggaaatgact tatatcttgc
     7561 gggtgatagg tgattcaatg attgatgagt accgcccggg agacatgatt tttgtcgatc
     7621 ctgaagtacc tgcctgccac ggtgacgacg ttattgcatt gatgcacgat acaggtgaaa
     7681 ccaccttcaa aaggttgata gaagatggga cacagcgtta tctcaaagcg ttaaacccaa
     7741 actggcctga gccttacatt aagatcaacg gtaattgctc tataattgga actgtgattt
     7801 tctcaggaaa accaagaaga tacaaaatca aagcctaatc aatgtttatg aacctgcttc
     7861 ggcaggtttt tttatacttg acaatgtacc tttgagatac ataatgtacc caagagaaac
     7921 aacaaacagg caggacgccc acgaagtagc cgcctggggc atatgaagtc caggatgatt
     7981 cgttagcaac aaaaaagcgc cctacaggac gcttagctct ttaacaatct ggtccccatc
     8041 aacaagtaac tgataacttg aggaggtgtg aaatgcacaa aacagaacca aaaatcgtcg
     8101 cgcccggata cacaaatgag gaaatttacg agtggatggc aaagaagctg gcagctataa
     8161 accagcttcg tgaagtgctg tcttatcgac aggtaacaat agactcctta aaaaaactgg
     8221 atcaggaaat cacggtttta tcacaggatg ttactttaga tattgtgcag acaaattagg
     8281 atcccattca ttttcgtcaa aatcatcaaa gtgatgaatt tgtgatctcc agtctcgata
     8341 atctaaaaat ttctgggcgg ttacgcttat tttatcaagc gtgagttcat cctgaattga
     8401 aagaagaagt tcatcaaatt tcatctcatt aatctgtttt ggcatccagt gatgcttcat
     8461 cagaataagg tgaaccagag cttttttccc attcaactga ttatagggag taccgaattt
     8521 cttccggtgc tcatgtaaga caaggtccag aagagtaagt aatgttgccc ttgattcaac
     8581 tttgcttatt tcgactgatg acactacccc actgatttca atgccccgat actttccaac
     8641 attttcacag tgggatttgt acagcgtata gatattaccg gacatttctt ttccttttgc
     8701 gttgttgggg ataaccagat taaccgaatc cttgttgttg gggaataacc aggtccacct
     8761 cgcctgatgt ggctaaaagc aggcacataa cagctaagta ttttcaacca gagagaatcc
     8821 ttagcgttgt ggtgaatgcg gctcagcgca cgcgggttaa ggttgaggct gacagtcgac
     8881 cttctgtgga tacccacccg tctggtgtgc aaccttcgcc aggcaccggg aggcacccgg
     8941 caccacaact ttatgctgtg tgtagtcttg acggtaccag tttgtaccct tgcttccggc
     9001 tggtaccgtc ctttttacaa aacagagaag agcatcaccg gacgacgggc tcataaccca
     9061 atccatccag gcggcagtca tcgcaggtgt tcttctctgt tttgtggaga aactaaccga
     9121 ccttgcaggg tcgatatgct gaagtaatcc tccaagcgtt tccacgttgc gtcgttatgc
     9181 taagcagaat ttgttttgtc caccggcaag gaaacaaggt cggttatggc gagtgcgtga
     9241 ggacgccgag ttagttggag agctggtaac accagtaatt aagaaaaatg actcattact
     9301 tttgcaacgg attttgagtg atggcagcca gaccgcgtaa gaataatgtt tcagtcccta
     9361 atttatatcc gctttatagc cgaaaggtaa ataaagtcta ttggcggtat aagcatccaa
     9421 taacagggaa attccatgct ttgggaacaa acgaagctga agctatcgct attgccactg
     9481 aagccaatac gcgcctggct gaacaaagaa cccggcagat tctggccatc agtgacagga
     9541 tcgcaaccag caaagggaaa gcaatcacaa cgtcaacttg gttagatcgc tatcaagcga
     9601 tccagaatga caggctgaaa agtggtgata taaagctcaa cacctacaag cagaaagcca
     9661 aacctgtctc cttacttaga gaacgggcag gattgaagct aatttcagct gttgatgtcc
     9721 gggatattgc acaattactt gatgagtata ttgccgctgg gcaaccgaga atggcacaag
     9781 tagttcgctc cgtcctgatt gatgttttca aagaggcgca gcactacgga gaagtacctc
     9841 ctggctataa ccctgcatta gctactaaac agccaagaag aaaaattacc cgacaacggc
     9901 tgagcctcga agaatggaaa aaaatattcg atatcgcaga tgccagccat cgttacatgg
     9961 gaaatgccat gctgctagcc ttggttactg gtcagcggtt aggtgatatt tcacgtatga
    10021 aatttagcga tatttgggat gatcatcttc atgtcgtaca agaaaagaca gggagcaaaa
    10081 tcgcaattcc gctttctctt cgcctcaatg cgattaactg gagtttacgt gatgtagtag
    10141 ctcgctgccg tgactatgca gtcagcccat acttagtgca ttttttccgt tcaacttcgc
    10201 aggcagaacg tggagcacag gtaaaatcca atacattgac gatgaatttt agtaaagcaa
    10261 gagatttagc agaaattaac tggggggaag gttcacctgc cacattccat gagcaacgat
    10321 ctttatctga acgcctttac aagcaacagg gattagatac acaaaaacta cttggtcaca
    10381 aaacacagca acaaaccgat cgttatcatg atgaccgtgg gaaaggctgg agcaaagtag
    10441 ctttgtgaaa attcaagcga ttcaacgcca tcttgatttt cttaacactc atgaaaaagc
    10501 ttcaaacagt ccgcttagag cgagaagcgg acattaaaat ctgcttctcc aaaaaatttt
    10561 cagggtctta gcgatatttt cagctcaaac actattcaca atgcggactt gggttctctc
    10621 attttctatc gtacagaaca actagtttta tgcttcacaa gctaattctc ttttattaat
    10681 ttcagggaat tacagagatt ggcaatatta gatttgcatt cgagaatagt tattgctttc
    10741 tttatttctc tgcatttatt taatcttgca ttgaatacta acctttcaaa attacttatc
    10801 agcttatttt gtggttctga gcagcgcagc ttatatagtt taaacaaccc agttctttga
    10861 tagtcatatc catactttga caaaaaggcc atcaggataa aatggaaaca gttaattaaa
    10921 tgccatttat tgcaagattt gtcactcatt atggtggata tgtacttgca aacctgtgta
    10981 tagtctaatt cagactctga tttagatttg aattctgaat aagtaataac tcctgtaggt
    11041 gtaatcattt tttccacaat actaataagg tcatccttga aaaaatggtc tacagcatgc
    11101 tcattatcat aaccagtccc actcaacatc gaagcatcgc aaaatatatt ttgcatggtt
    11161 ttaatatact tcacaaaata aagatcgttc ttttctgaaa gtgttacatc taaatatatc
    11221 aactctcttt cgaactttat acaccaagca taaaatgcta aaaatcgata acaaaaagtt
    11281 tgtaggtaat atttatcacc aattgattca ttatttttga aattatgcca tcctttagtg
    11341 caatttcctg aaaaattcca caacctatga ttcaatgact cagctgaatc gagtaaaggc
    11401 attttatatt ttgatatcgc ctctttgatt ttcttctttt gttcgtagct atgttcaact
    11461 ttgattttgt aaacaatgaa atatttattc cataaaggtg tgcatacacc ttttataaaa
    11521 aataataaaa ccgtaactaa tgcagatact agtgccgcca taacagcagg agctgaagtt
    11581 atttcagaca tatggaacct tattgaatgt ttattcagat atctatttgt atgtaagata
    11641 ttaatataaa aacgctctta tcacaataga gtaaataatt cctctcctgc ttttacagca
    11701 agtatcatct gtaacttagg ctcaattcag tacatgacga tccactccct gaaaatttgc
    11761 attatgagtt gctgtcaacg tccgcttctg gcacggcgcg gacgagctag cgttgctgag
    11821 ggctccttgt gagcgagaag tagacgcaac cagggtggcg taagtccaat ttgctaaaaa
    11881 tagagcatag aagccagttc aaatcatgga tgcccaatcg gcatattggt agtattgcgg
    11941 tacaacggaa aatgccattc aaaggttgaa ctatgggtac tgtaatttct cttgaagtgt
    12001 caggaatggg gctagattgg agtaagaact cgctgggtat agatcatgga ggcctgtttc
    12061 aaagtgccga tcatcacgtc aaaccactaa atgaagatca aatagaggaa ggagaggact
    12121 tcgacactcc tgatggcatc ctctgtcgta ctgtgttgcg taagcctttg ggacaagttg
    12181 cctaccgatt ggaattgttg ggtttcaccc ttgaaaatat acgctatgag tatgaactca
    12241 tggctaaaga ttcaattgaa taccaagagg aatttaacga atcctgtccg gaatatgcaa
    12301 agccaattag taacatgatg agctttgatg aattcgttaa cttcatcaag agcgtaaata
    12361 ttagcgagct aaacgatgat tatattagct taaaagatag aataaaagaa cgcgagctta
    12421 tcatgggccg atttaataat gatgaattat taagcaggat accccaatac gacaatgcct
    12481 ttgataatgc ttggtctgaa aagtctgctt ttgggacttt agttagcata ctacatccat
    12541 actcagtgat gcgacttttg gccgaaaatc caaataatca taatgaattc gtcacttggg
    12601 attacggaga tctagtttct gctggttatg cgaatataaa agatattcag gtaaatgctc
    12661 gcagacgtga caagttcctg attgccaccg agggaagcac cgactccaac gtcatcaaat
    12721 acgcattgca cctcttacgg cctgatgtag aagatttttt tcactttatc gacatgagtg
    12781 aagggtaccc gttcactggt tcaggaaatc tggttaagtt cgctgctggg ctcgtaaaaa
    12841 tcgacattca aaacttgatt gttttcgtgt tagataatga ttgcgaaggg attgaggcat
    12901 atgaaaaaat ccgcaaactc gcattgcctc acaatattag ctgcattcat cttcctaatc
    12961 ttcctcattt caatcaattt ccagctattg gtcctcacgg cgttacctat gaaaacatca
    13021 atggccgggc tgtagcgatt gaatgctatc tggatctaag attttctgat tatgatgaag
    13081 cgaaaatccg gtggtcaaat tacaaaaagg aaagtgatgc ttatcaagga gcgctggaaa
    13141 ataaagacta ttacacgaaa aaattcatga aactaaaacc aggaagtaag ggttttagca
    13201 attatgattt tgccaaactc gctattgtct tggatgaatt aataaaagcg tgctcaaaaa
    13261 tctcatcaaa gattagaaaa aatgaagtag atgttcaata tccagactaa aaaggccgca
    13321 tgcgcggcct gtatgagtta accgatattg cgatggccta tgaggtacat cactcgctgg
    13381 taatgttcct gccggttgcg gtgaagttgt aatccctgct cccaggcaat atctgcaacc
    13441 ctctccgcat cgaatccggt gcgctcattt tcgcccctag attgccagag tttcaaaatc
    13501 tcagtattca cgttcatttt ctgtttcctg tgtgtttggg aactttcagc gtcgtgtcac
    13561 gactgtggga acgccaacat aatttggtgc gggcaacgaa atatcctcat ccactgttaa
    13621 cgtgaactga aagtttgacc gttcccggtt gattaacaca ttttgatgcc agcaatgtcc
    13681 gctcctggca caggacagac cttaaatatt aacgagcatc cagcagaaat aataaaaagc
    13741 tgaggaaatc ctaatgggaa gctggcagta gaactgccat ctgaccggcc agcctacccc
    13801 aaactaccaa ggggttttga taacttattt tgataaaatt ttgataaccg ttagtagcct
    13861 aacaaaaaac aacgagagcg ttacgctccc gttaataaat ttaacaaact acggcatcac
    13921 atgttcttga tgatcgcgtc gccaaactcg ctacatttca gcaatttagc gccttccatc
    13981 agacgttcga agtcataggt tacagtcttg gcgttgattg cgccttccat acctttaaca
    14041 atcaggtctg cggcttcagt ccagcccata tggcgcagca tcatctcagc ggagagaata
    14101 atagagccag gatttacttt gtcctgaccg gcatatttcg gcgcagtacc gtgggtggct
    14161 tcaaacaggg cgcattcgtc accgatgttt gcaccagggg cgataccgat accgccaacc
    14221 tgcgctgcca gggcgtcaga aatgtagtca ccgttcaggt tcatacaggc gataacatca
    14281 tattcagccg gacgcagcag gatctgttgc aggaatgcat cagcaatcac gtctttaatg
    14341 acgatctctt tgccagtgtt cgggttttta actttcagcc acgggccacc gtcgatcagt
    14401 tcaccgccaa actcttcacg cgccagctgg tagccccagt ctttaaacgc tccttcggtg
    14461 aacttcatga tgttgccttt gtgcaccaga gtcacagagt cacgatcgtt agcaattgcg
    14521 tattcgatcg ctgcacgaac cagacgtttg gtgccttctt ccgaacacgg cttaataccg
    14581 ataccacaat gttccgggaa gcgaattttc ttcaccccca tctcttcacg caggaattta
    14641 atcactttct cggcgtcggc agagtctgct ttccattcga tacccgcata aatgtcttcc
    14701 gagttttcac ggaagataac catatcggtc agttcagggt gtttaaccgg gcttggagtg
    14761 ccctgatagt aacgtaccgg acgcaggcag atgtagagat ccagttcctg gcgcagggca
    14821 acgttcagag agcgaatacc gccaccaacc ggagtggtca gcggaccttt aatggcaacg
    14881 cgatattcac gaatcagatc aagagtttca gcaggcagcc agacgtcctg accataaacc
    14941 tgtgtggatt tttcaccggt gtaaatttcc atccaggaga ttttacgctc gcctttatag
    15001 gctttctcga ctgcagcgtc gaccactttc agcatggctg gggttacatc tacaccgatt
    15061 ccatcacctt caatgtaagg gataatcgga ttttcaggaa cgttgagttt gccgttttgc
    15121 agggtgatct tcttgccttg tgccggaaca actactttac tttccattca cctctccttc
    15181 gagcgctact ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt cctgcgatac
    15241 ggatgcttta gagcaatttt ttgttaatga tttgtaattt gcttgtcaat actaccctat
    15301 tgtttgccgt catgaaagat gcgcgttatt aggctataat gcggcaattc ataatctctg
    15361 aaaataccat gcaaaaaact tcttttagaa atcaccaggt taagcgattc agctcgcaac
    15421 gttctaccag gcgtaaacct gaaaaccagc ccacgcgtgt gatcctgttc aataaaccct
    15481 acgatgttct tccgcagttc accgatgaag ccggacgcaa aacattaaaa gaattcatcc
    15541 cggttcaggg cgtttatgca gcaggtcgcc ttgaccgcga tagcgaaggg ttgctggtgc
    15601 tgaccaataa cggcgcgttg caggcgcgtt taacccagcc gggtaaacgc accggaaaaa
    15661 tctattatgt gcaggtggaa ggtattccca cacaagacgc acttgaagcc ttgcgcaatg
    15721 gcgtaacctt aaatgatggc cctaccctgc ccgccggcgc ggagctggtt gacgaacccg
    15781 cgtggttatg gccgcggaat ccaccaattc gtgaacgcaa aagtattccc accagctggc
    15841 tgaagatcac cttatatgaa ggacgtaatc gccaggtgcg ccgcatgacc gcccatgttg
    15901 gcttccccac gctgcgactg attcgctatg cgatgggtga ttactctttg gataatcttg
    15961 ccaatggcga atggcgagaa gtgacagatt aaggatgcaa aatgtttaaa ccgcacgtta
    16021 ccgttgcttg cgtggtgcac gcagaaggca aatttttagt cgttgaagag acgattaatg
    16081 gtaaagcgtt atggaaccaa cctgccgggc atctggaagc cgatgaaacc ttagtggaag
    16141 ccgccgcccg tgaactgtgg gaagaaaccg gcatcagcgc gcagccgcaa cactttattc
    16201 gtatgcatca gtggattgcg ccagataaaa cgccgttttt gcgtttcctc tttgccattg
    16261 agcttgagca aatatgcccg acccagcctc atgacagcga tatcgactgc tgccgttggg
    16321 tcagcgccga agaaatttta caggcgtcaa atcttcgttc gccgctggtg gcggaaagta
    16381 ttcgttgtta tcaaagcggg caacgttatc cgctggagat gattggcgat tttaactggc
    16441 cttttacaaa gggtgtcatc taaaaggctg cgtgatagaa tacgccgcct tgaagttcaa
    16501 tgtcgtgagt gatccaatgt ctgaaaccgc aaaaaaagta atcgtcggca tgtccggcgg
    16561 tgtcgattcc tccgtttctg cctggctgtt gcaacaacag ggatatcagg tcgaaggcct
    16621 gtttatgaag aactgggaag aagacgacgg tgaggaatat tgcacagcgg cagcggatct
    16681 ggctgatgcc caggctgtct gcgacaagct cggcattgaa ctgcacaccg ttaactttgc
    16741 tgccgagtac tgggacaacg tcttcgaact gttccttgcc gaatataaag ccggtcgcac
    16801 gccgaatccg gatattctgt gcaacaaaga gatcaaattt aaagccttcc tcgaatttgc
    16861 cgccgaagat ttaggtgccg attatatcgc taccggtcat tacgtacgtc gggccgatgt
    16921 cgatggcaag agccgcctgc tgcgtggtct ggacagcaat aaagaccaga gctacttcct
    16981 ttatacgctc agccatgagc agattgcgca aagcctgttc ccggtcggcg aactggaaaa
    17041 accgcaggtg cgtaagattg ctgaagatct tggtctggtc accgcgaaga aaaaagactc
    17101 taccggcatt tgcttcattg gcgaacgtaa attccgcgag ttcctgggcc gttatctccc
    17161 ggcgcaaccg ggcaaaatca ttaccgtcga tggcgatgaa attggtgagc accaggggct
    17221 gatgtatcac actctcggtc agcgtaaagg tctgggtatc ggtggcacca aagaaggtac
    17281 cgaagaaccg tggtatgtgg tggacaaaga cgtcgaaaac aacattctgg ttgtcgctca
    17341 gggccatgaa cacccgcggc tgatgtctgt cgggttgatt gcccagcagt tgcactgggt
    17401 cgatcgcgaa ccattcaccg gcactatgcg ttgcacggta aaaacccgct atcgccagac
    17461 cgatatccct tgcaccgtca aggcgctgga cgatgatcgc attgaagtga ttttcgatga
    17521 accggttgcc gccgtgacgc cgggccagtc tgccgtcttc tataacggtg aagtgtgcct
    17581 cggtggcggt attattgagc agcgtctgcc gctgccggtc tgattattat ctttacttaa
    17641 aacaaggaag cagtgaacgt ggcaaagaat tactatgaca tcaccctcgc cctggccggt
    17701 atttgtcagt cggcacgcct ggtgcaacaa ctcgctcacc aggggcattg tgatgccgat
    17761 gcgctacacg tctcactcaa cagtattatt gatatgaacc ccagctcgac gctggcggtt
    17821 tttggcggta gcgaagccaa cctgcgcgtc gggctggaaa ccctgctcgg cgtgctcaat
    17881 gccagcagtc gccagggctt aaacgccgaa ttaacccgct acacactcag cttgatggtg
    17941 cttgagcgca aactctcctc agcgaaaggc gcgctcgaca ctctgggcaa ccggatcaac
    18001 ggcctgcaac gccagctcga acacttcgat ttacagtccg aaacgctgat gagcgcgatg
    18061 gctgctatct atgttgatgt gattagcccg cttggcccgc gcattcaggt caccggttcc
    18121 cctgctgtac tgcaaagccc acaagtgcag gcgaaagttc gcgcaaccct gctggcaggc
    18181 attcgcgccg ccgtgctctg gcaccaggtc ggcggcggac gtctgcaact gatgttttct
    18241 cgtaatcgcc tgaccactca ggcaaaacaa attcttgctc atttaacccc ggagttgtga
    18301 tctatggaat tatcctcact gaccgccgtt tcccctgtcg atggacgcta cggcgataaa
    18361 gtcagcgcgc tgcgcgggat tttcagcgaa tatggtttgc tgaaattccg tgtacaagtt
    18421 gaagtacgtt ggctgcaaaa actggccgcg cacgcagcga tcaaggaagt tcctgctttt
    18481 gctgccgacg caatcggtta ccttgatgca atcgtcgcca gtttcagcga agaagatgcg
    18541 gcgcgcatca aaactatcga gcgtaccact aaccacgacg ttaaagcggt tgagtatttc
    18601 ctgaaagaaa aagtggcgga gatcccggaa ctgcacgcgg tttctgaatt catccacttt
    18661 gcctgtactt cggaagatat caataacctc tcccacgcat taatgctgaa aaccgcgcgt
    18721 gatgaagtga tcctgccata ctggcgtcaa ctgattgatg gcattaaaga tctcgccgtt
    18781 cagtatcgcg atatcccgct gctgtctcgt acccacggtc agccagccac gccgtcaacc
    18841 atcggtaaag agatggcaaa cgtcgcctac cgtatggagc gccagtaccg ccagcttaac
    18901 caggtggaga tcctcggcaa aatcaacggc gcggtcggta actataacgc ccacatcgcc
    18961 gcttacccgg aagttgactg gcatcagttc agcgaagagt tcgtcacctc gctgggtatt
    19021 cagtggaacc cgtacaccac ccagatcgaa ccgcacgact acattgccga actgtttgat
    19081 tgcgttgcgc gcttcaacac tattctgatc gactttgacc gtgacgtctg gggttatatc
    19141 gcccttaacc acttcaaaca gaaaaccatt gctggtgaga ttggttcttc caccatgccg
    19201 cataaagtta acccgatcga cttcgaaaac tccgaaggga atctgggcct ttccaacgcg
    19261 gtattgcagc atctggcaag caaactgccg gtttcccgct ggcagcgtga cctgaccgac
    19321 tctaccgtgc tgcgtaacct cggcgtgggt atcggttatg ccttgattgc atatcaatcc
    19381 accctgaaag gcgtgagcaa actggaagtg aaccgtgacc atctgctgga tgaactggat
    19441 cacaactggg aagtgctggc tgaaccaatc cagacagtta tgcgtcgcta tggcatcgaa
    19501 aaaccgtacg agaagctgaa agagctgact cgcggtaagc gcgttgacgc cgaaggcatg
    19561 aagcagttta tcgatggtct ggcgttgcca gaagaagaga aagcccgcct gaaagcgatg
    19621 acgccggcta actatattgg tcgagctatc acgatggttg atgagctgaa ataaacctcg
    19681 tatcagtgcc ggatggcgat gctgtccggc ctgcttatta agattatccg ctttttattt
    19741 tttcacttta cctcccctcc ccgctggttt atttaatgtt tacccccata accacataat
    19801 cgcgttacac tattttaata attaagacag ggagaaataa aaatgcgcgt actggttgtt
    19861 gaagacaatg cgttgttacg tcaccacctt aaagttcaga ttcaggatgc tggtcatcag
    19921 gtcgatgacg cagaagatgc caaagaagcc gattattatc tcaatgaaca tataccggat
    19981 attgcgattg tcgatctcgg attgccagac gaggacggtc tgtcactgat tcgccgctgg
    20041 cgtagcaacg atgtttcact gccgattctg gtattaaccg cccgtgaaag ctggcaggac
    20101 aaagtcgaag tattaagtgc cggtgctgat gattatgtga ctaaaccgtt tcatattgaa
    20161 gaggtgatgg cgcgaatgca ggcattaatg cggcgtaata gcggtctggc ttcacaggtc
    20221 atttcgctcc ccccgtttca ggttgatctc tctcgccgtg aattatctat taatgacgaa
    20281 gtgatcaaac tgaccgcgtt cgaatacacc attatggaaa cgttgatacg caataatggc
    20341 aaagtggtga gcaaagattc gttaatgcta caactctatc cggatgcgga gctgcgggaa
    20401 agccatacca ttgatgtact gatgggacgt ctgcgcaaaa aaattcaggc acaatatccg
    20461 caagaagtga ttaccaccgt tcgcggccag ggctatctgt tcgaattgcg ctgatgaaaa
    20521 aattactgcg tctttttttc ccgctctcgc tgcgggtacg ttttctgttg gcaacggcag
    20581 cggtagtact ggtgctttcg cttgcctacg gaatggtcgc gctgatcggt tatagcgtca
    20641 gtttcgataa aactacgttt cggctgttac gtggcgaaag caatctgttc tatacccttg
    20701 cgaagtggga aaacaataag ttgcatgtcg agttaccaga aaatatcgac aagcaaagcc
    20761 ccaccatgac gctaatttat gatgagaacg ggcagctttt atgggcgcaa cgtgacgtgc
    20821 cctggctgat gaagatgatc cagcctgact ggctgaaatc gaatggtttt catgaaattg
    20881 aagcggatgt taacgatacc agcctcttgc tgagtggaga tcattcgata cagcaacagt
    20941 tgcaggaagt gcgggaagat gacgacgacg cggagatgac ccactcggtg gcagtaaacg
    21001 tctacccggc aacatcgcgg atgccaaaat taaccattgt ggtggtggat accattccgg
    21061 tggagctaaa aagttcctat atggtctgga gctggtttat ctatgtgctc tcagccaatc
    21121 tgctgttagt gatcccgctg ctgtgggtcg ccgcctggtg gagtttacgc cccatcgaag
    21181 ccctggcaaa agaagtccgc gaactggaag aacataaccg cgaattgctc aatccagcca
    21241 caacgcgaga actgaccagt ctggtacgaa acctgaaccg attgttaaaa agtgaacgcg
    21301 aacgttacga caaataccgt acgacgctca ccgacctgac ccatagtctg aaaacgccac
    21361 tggcggtgct gcaaagtacg ctgcgttctc tgcgtagtga aaagatgagc gtcagtgatg
    21421 ctgaaccggt aatgctggag caaatcagcc gcatttcaca gcaaattggc tactacctgc
    21481 atcgtgccag tatgcgcggc gggacattgc ttagccgcga gctgcatccg gtcgccccac
    21541 tgctggacaa tctcacctcg gcgctgaaca aagtgtatca acgcaaaggg gtcaatatct
    21601 ctctcgatat ttcgccagag atcagctttg tcggtgagca gaacgatttt gtcgaggtga
    21661 tgggcaatgt gctggataat gcctgtaaat attgcctcga gtttgtcgaa atttctgcaa
    21721 ggcaaaccga cgagcatctc tatattgtgg tcgaggatga tggacccggt attccattaa
    21781 gcaagcgaga ggtcattttc gaccgtggtc aacgggttga tactttacgc cctgggcaag
    21841 gtgtagggct ggcggtagcc cgcgaaatca ccgagcaata tgagggtaaa atcgtcgccg
    21901 gagagagcat gctgggcggt gcgcggatgg aggtgatttt tggtcgccag cattctgcgc
    21961 cgaaagatga ataaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
    22021 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
    22081 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
    22141 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagtcgaca
    22201 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggtccg ttcgaaagct
    22261 acgatcatct cggtgaaact aactggtcat tgttagtgca agcagtaaat cactggcatg
    22321 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
    22381 tgatgatctc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
    22441 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
    22501 aacagcactg cccacacccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
    22561 aagagctgga gcctggcgat attctttata ttccgccagg attcccgcat gaaggctacg
    22621 cgctggaaaa tgcgatgaac tattccgtgg gctttcgcgc gccaaatacg cgggaactga
    22681 ttagtggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
    22741 cggatgttcc acctcgcgct catcctgcgg atgttctgcc gcaagagatg gataaactgc
    22801 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
    22861 ttatatccca gtcacgtcat gaactggata tcgcgccgcc agagccgcct tatcagccgg
    22921 atgaaatcta cgatgcgctg aaacaaggtg aagtgctggt gcgcctgggt ggtctgcgcg
    22981 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
    23041 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
    23101 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
    23161 tcgaagggta agtttggatt taagccggat gcggcatcac atgccgcatc ccttttcgct
    23221 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
    23281 ccttccagag tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc
    23341 gggcacggta atcccataaa cgacaactgc gcgccgtcgg taccaccgcg gatcggtttc
    23401 agttccggtt caatatcgca atcgcgcatc gcctgctggg cgatatcgag aatatgcgga
    23461 tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccag ttcaatgtag
    23521 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
    23581 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
    23641 gtgcctttca tgctcgccag atgatagaaa ccttcatagc cttctgtcat ttccgggctt
    23701 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
    23761 cctttcgccg tgcccggatg aacattgtta ccgacaattt tgatattgac cgacgcggcg
    23821 ttgaagtttt caaactccag ttcgcctacg ccaccaccat caacagtgta agcccagcgg
    23881 gcatcgaagg cgtcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    23941 aaggcgacgc gaatatcacc atgcggaatt tttttctgtt gcaataccgc cagcgcggtc
    24001 atgatttctg caatacctgc tttgtcatcg gcacctaaca aggttttacc atcggtggta
    24061 atcagcgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    24121 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattcaca
    24181 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    24241 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    24301 ttgataagcc ccatctcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    24361 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc
    24421 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    24481 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    24541 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    24601 ttcaagcggt ttgccgtaga attaccgccc tcattaagag tcaccaaggt ggttaaccac
    24661 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    24721 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    24781 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    24841 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    24901 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    24961 gctatgtgaa cactgttttc caaagctacg cacttttccc ccacatgacc gtgttcgaaa
    25021 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    25081 tggaagccct gcggatggtg caactggaaa ccttcgctca acgtaaaccg catcagctct
    25141 ctggtggtca acagcaacgc gtcgccattg ctcgcgcggt ggttaacaag cctcgtctgt
    25201 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    25261 agctgaaagc gttacagcgt aagcttggta ttaccttcgt ctttgtcact cacgatcagg
    25321 aagaagcgct caccatgtca gacaggattg tggtgatgcg cgagggtcgt attgagcaag
    25381 atggtacacc gcgcgaaatt tacgaagagc cgaaaaacct gtttgttgcc ggcttcattg
    25441 gcgaaatcaa tatgtttaac gccactgtca tcgaacgtct agacgagcag cgcgtacgcg
    25501 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    25561 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    25621 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    25681 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    25741 accctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    25801 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    25861 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    25921 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cgctggataa ctacacgcgt
    25981 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    26041 accctcgcct gcctggtgct gggctacccg tttgcctggt ttctggcgaa gctgccacac
    26101 aaggtgcgtc cgctgctgct gtttctgctg attgttccct tctggaccaa ctcactgatt
    26161 cgcatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    26221 tggctgggcg ttatcgacac accaattcgt atcatgttca cgcccagtgc ggtgattatc
    26281 ggtctggttt acattctgct accgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    26341 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    26401 atccgtatca ttattccact gacgatgccg ggaattattg ccggatgttt gctggtgatg
    26461 ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg
    26521 atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccatt tggtgcagcc
    26581 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    26641 cgtctgctga ataaaaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    26701 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    26761 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    26821 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    26881 cgacatttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    26941 gtggtaagcc gttcgttagt ggaatgctgt ttgtggtgat gatgtcacca gatatcgtga
    27001 tggcgatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    27061 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacagtg tattcgcgcc
    27121 tgaaaggttt tgacgtgcga atgctggaag cggcgaaaga tctcggtgcc agcgaattta
    27181 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    27241 taagctttac cctgtcgatg gacgatgtag tagtttcttc gttcgtcacc ggaccaagct
    27301 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    27361 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    27421 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    27481 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    27541 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacaattcac
    27601 caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
    27661 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    27721 cgataaaatg cgtaaagaag ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    27781 cagcaatctc gatccagaca tgctcaataa gccgtttgac ccgaataacg attactccat
    27841 tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
    27901 tgtcaccagc tgggccgatc tgtggaagcc tgagtacaaa ggcagcctgc tgttgaccga
    27961 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac
    28021 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtggc
    28081 agcctttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    28141 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgccaattg acgtggtatg
    28201 gccgaaagaa ggcggcattt tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    28261 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    28321 tgccgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagcccgga
    28381 agtggcgaac gataaaacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
    28441 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    28501 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    28561 gctaatgagt caggacagta aagttttctt tcgtatattc ctcggtatcg gtctggtact
    28621 gatcctcatt tctgttgttg tcttttataa ccagttcacc tatagcaaag acgcgattca
    28681 taccgaaggc gtcattgttg ataccgtctg gcactccagt cattctcatc gcacgggtaa
    28741 agatggttcg tggtatcccg tcgttgcctt tcgaccgacg ccagattaca cgctaatttt
    28801 caattcgagt atcggcagcg atttttatga agacagcgaa ggcgataaag ttaatgttta
    28861 ttattctccc gggcatcctg aaaaggctga gataaataac ccgtgggtta atttttttaa
    28921 atgggggttt atcggcatta tgggcgttat ttttatcgcc gtcggtttat taataagtat
    28981 gccctcctct aaaaaatcac gcaggaaacg gaagtcccgc ccatgaaaaa attcattata
    29041 ctgctcagtt tactgatact cctgcctctg acagcagcca gcaagccatt aattccaata
    29101 atgaagacac tttttacgga cgttaccggt actgtaccag atgctgaaga gatcgcgcat
    29161 aaagccgagc tttttcgcca acaaacggga attgcaccgt ttattgtggt tttacccgac
    29221 attaataatg aagccagtct cagacaaaat ggtaaagcga tgctggcgca tgcgtcatct
    29281 tcattgagtg atgtaaaagg aagtgttctg ttactattta ctacccgcga accacggtta
    29341 attatgatca ccaacggcca ggttgaaagc ggtctggacg ataaacatct cggccttctg
    29401 atagaaaatc acacactggc ttatttacat gctggcctct ggtatcaggg aatcaataat
    29461 gcattggctg ttctacaagc acagatatta aaacaaccga cgcctccact aacatattat
    29521 ccgcatccag gaaaacagca tgaaaatgat ccccccggca gcaccaacac attaggtttt
    29581 atcgcctggg ctgcaacgtt tgtcttattt tcctcggtct tttactacac gactcgtttt
    29641 atttatgcgt taaaatttgc agtagcgatg actattgcca atatgggcta tcaggcttta
    29701 tgcctgtata tcgataatag ctttgcgatt accagaatat cgccactctg ggcagggctg
    29761 attggtgtct gtacgtttat tgccgtgttg ttattgacga gtaaacggta aaagagacag
    29821 taaaatgcgt gtgtattatg ccggttgcgg cgcgaacgcc ttatccggcc cacaaaaccc
    29881 gcaaattcaa ttaattgcgt ccccttgcag gcctgataag cgtagtgcat caggcaatgc
    29941 ttcccgcttt taatcccttc agcaactttt caacaaactc aggcaccacc tggcttgccg
    30001 ggccgtaata tttctcggca aattcattac caacctgact cggttcaaga ttcagttcca
    30061 cggtgtgcgc gccatgcagt ttcgcttcgt gaacaaaccc agccgccgga taaacatgcc
    30121 cggatgtgcc aatggcaatg aaaatatcgg ccatcgacaa cgccatataa atttcatcca
    30181 tgccgagtgg catttcgcca aaccacacta catgcgggcg caagggtgcc ggaaactggc
    30241 aacaatggca tttatcttct ggggtaacgt ctcctgtcca gtcgagaacc tgaccacttt
    30301 gtgaacaacg cactttcagc agttccccat gcatatgaat cacattggta ttacctgcgc
    30361 gttcatgcag gttgtcgata ttctgcgtca ccagcaaaaa gcgatcgccg agggcgtctt
    30421 gcagtttagc cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc
    30481 gacgacgggc gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg
    30541 ttgccacatc ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa
    30601 tacctgattc cgcagaaatt cctgcccctg tcagtacgag tactcttggt ttttccattg
    30661 cttccggcac cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc
    30721 gtttattttt acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg
    30781 ttgtttaatc ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggc
    30841 gcgcgcgttc aatgcgcgga acacgagcta caggtaagag atgacgaggc agcctgtccg
    30901 ccagttgcgc tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag
    30961 ggtcaacaat ggtcaggata tttcccagac aaaccgctaa taaatccaga taacgctcaa
    31021 cgtgcgccct tgcctgctca tcgccttgat cataaagcgc aataatttcg ggagcctgca
    31081 acggttgatg ataatagtgt tgatacagcc acgcaaaacc gcgaccagac agataatttt
    31141 caatgcagcc atgctgacca cagccgcagc ggcgtaacgg gaaatccaac cccatcatgg
    31201 ttaacgcatc aaccggcaga cgcatatggc caaactcgcc ggtaatgtag cttttaccgg
    31261 taatcggttt gccgttgaaa atcagcccgc cgccaacgcc ggtgccgaga atcaacccca
    31321 tcaccagtgg atattgagta aattcatcat cccaggcttc tgaaagggca aaacagttgg
    31381 catcgttatc aaggcgtaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt
    31441 taccgctggc agcggggaca ttggcggcat acagcgtacc atcttctgtt tccggcattc
    31501 ccggaatacc gatgccgaca gaacctttac agccaaaacg ttggtcagct tcagctacca
    31561 gctcacacac tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt
    31621 cccactgcaa ctgccgaccg ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat
    31681 caaacccgta atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg
    31741 actcgcgcag ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc
    31801 agcaacaatg ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg
    31861 aaatcaataa aatagatatc gctggagagg aactgatgac cgatcaactt ttcaatccac
    31921 tcaataatcg gggtaagttg cagtgaaacc actacgccga taatcacacc acacaggctg
    31981 ccgaacagcc ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc
    32041 gcccccagcg ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag
    32101 gtggagacga tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg
    32161 gcgcggatca tttggatatc gcgatacata tagccgtaag taccaatcca gcttttaata
    32221 taaacatagc tgttggtcac ttcacccgca tcgcgtacca gcttattggc gttgaaaaca
    32281 tccgtcattt taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca
    32341 tctgccagcg ggatcatggc aaaactgtga tcgagttgac cactcaactg caaaataccg
    32401 gcaacgtgca aacgcacacg ttttggctgc atcagtttat gctcgggatt cgagttgggg
    32461 atcataatcg acacccaatc cccctgcttc actttcagcg catccgccac gcctttgccg
    32521 atgataattt gctgttcgcc cgctttaaaa ttgcgccacg catcgccctg gacaaacgag
    32581 ggtaatgcgc tcagacgctg ttcctgttgc gggttaacgc ccttcacctg gattgcgcga
    32641 agattcgcgc cactttccac cagcccggtg aaattgatat acggcgcggc agcggcaata
    32701 cccggcactt tttgcacgtg atccagtgct tcctgccagt tagtccacgg ctgatccacc
    32761 gcctcgattt caccatgcgg caccaccgcc agaatacggt tgttcagttc gcgttcaaag
    32821 ccgttcatcg cgcttaagcc gacgatcaat accgccacgc cgagggcaat gccaatggta
    32881 gaaataacgg agatcagcga caccatgccg ccgcgccgcc gtccgcgact aaaacgcagg
    32941 ccaattaata acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg
    33001 tcagacgacc atcacgcatt tctaactggc ggctcatacg tttcgccagt tgcaggtcgt
    33061 gagtaaccac caggaaggcg gtgccctgca agcgattcaa ttccccaagc aactggaaga
    33121 tgctgtcggc gttacgcgca tcgaggttac cggtaggttc atccgccagt accaggcgcg
    33181 ggttattcac cagcgcacgg gcaatcgcca cacgctggcg ttcgccgcca gaaagttcag
    33241 atgggcggtg attcgcacga tgatccagcc ccaccgcttt taacatctca agtgcacggc
    33301 tgttgatttc agcgggcttt ttcttgccaa tcagcagcgg catagccacg ttttccaggg
    33361 cggtaaaatc cggcagcagg tgatgaaact gataaataaa gcccagcttc tggttgcgca
    33421 gttcagcttt cgctgccgac gacagtttgc tcattggctg accattaaaa atcacatcgc
    33481 cggaggttgg cgtatccagc ccgcccagca ggtgcagcaa ggtactttta ccggaaccag
    33541 agctaccgac gatcgccatc atttcacctt cgccgacgct gaaactgaca ttgtgcagca
    33601 catcggtttg cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga
    33661 tcttattcat aacgtaaagc ctcagcgggt tgagtggcgg cagcgcgcca tgaagggtaa
    33721 agcgtagaca gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt
    33781 tcgatagcca ccggcagcgc cgcgccatca agcaggacgc cgattatcgg catcagatta
    33841 tttaactggc tggcaagcag ggcgccaagc gccgctccga ggatcgcacc gataatcccg
    33901 gcgctggccc cttgcaccat aaagaccatc atgatttgtc gcggagttaa gccttgcgtt
    33961 tgcaggatcg ctacttcgcc ctgcttctcc attaccatca gccctagtga ggtaataatg
    34021 ttaaacgccg caacggcgac aatcaggctc agcagtaaac ccatcatatt tttttccatg
    34081 cgtacggcct ggaacaactc gcctttacga tcacgccagt cctgccattt gctgccttca
    34141 ggcagttttt gctgacttaa tgagtcgact ttcagcggct catccagcca caaacgccag
    34201 ccggtaatat tgcctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc
    34261 atttcatagc catcgacttc actgttggcg gcgaaagtgc caatcacatt gaacaggcgc
    34321 tggcttggaa tacgccccat cggcgtgaac tggctggcag atggtaccat cacgcggatt
    34381 tgatcaccgc gattaacgcc tagctgtgag gcaagttgtt cgccgaggat gacattatat
    34441 ttccccggct cgaggtcagt ttgtttcaca ttgaccagat acggtgtaag tggatctttt
    34501 tgcgccgggt cgataccgag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc
    34561 acatcaccgg tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttct
    34621 gggagttgct gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg
    34681 ccaaggatgt tgttttgcag ctcgcgctca aagccgttca tcactgacaa tactgtgacc
    34741 agcgccatca ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag
    34801 cgatccgctg cacgcccacg catgtaacgc aggccaataa atagagcgac aggttggtac
    34861 atgaaatccg tctggttgct gtagcaaaga cacgagtata taagcgaaag caagatgggt
    34921 aaacgtctga actgtaagtg atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg
    34981 gctaaattgc catgttttat catcaatctt gccgcctgat cttaatctga aaaatcatcc
    35041 aatacagaca gatacccatt acacctcggg tcataacccg caggatcaac cgcatgtaaa
    35101 ttaaaaaaga gcgtgttgtc ctgatgaaac aaaaagagct atggattaac cagatcaaag
    35161 ggttatgtat ttgtctggtg gtgatttatc actcggtcat taccttttat ccgcatctga
    35221 ccacttttca gcatccgtta tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt
    35281 accttgcccc ctttcgtatg ccggtttttt tctttatatc cggctatttg attcgccgct
    35341 atatcgacag cgtaccgtgg ggaaattgtc tcgataaacg gatctggaac atcttctggg
    35401 tgctggcgct ttggggcgtg gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg
    35461 cacctgagcg cgatttaagc aatgcctcca atgccgctta tgccgattcc accggtgagt
    35521 tcctgcacgg gatgatcacc gccagcacca gcttgtggta tctgtatgcg ttaattgtct
    35581 atttcgtggt atgtaaaatt tttagccgtc tggcgctgcc actattcgcc ttgtttgtac
    35641 tgctgagtgt ggcggttaat ttcgttccca cgccgtggtg gggaatgaac agtgtgatcc
    35701 gcaatttgcc ttattacagc cttggcgcat ggtttggcgc aacaataatg acctgtgtta
    35761 aagaggtgcc gttgcgccgc catctgctga tggcttcttt gcttaccgtt ctggcggtcg
    35821 gtgcctggtt gtttactatc tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga
    35881 agctgtttta tcaatacgag caacgattcg gtatgcgctc caccagcctg ctgaatgtga
    35941 ttggctccaa caccattgct atctacacca cccatcgcat tctggttgaa atattcagct
    36001 taactctgct tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc
    36061 tgctggttta tccctttgtc agtttgttca tctgtactgt tgcgggcttg ctggtaagaa
    36121 aactttcaca gcgtgcattc agcgatctgt tgttctcccc gccttcactg cccgctgccg
    36181 tcagttactc ccgctaagcc atcgccccag ccggggcgat tcaatttgct aaaccatgtc
    36241 gattacggat aatgaacagc attgcttatc agagatatgc ccccatatgt tgaggcatat
    36301 cctaactaga atctgacaac cgttatgcct gaacaatatc gttatacgct gcccgtcaaa
    36361 gcgggtgagc agcgtctgct gggcgagtta accggcgcag cctgtgcaac gctggtagcg
    36421 gaaattgccg aacgtcacgc cggtccggtg gtactcattg caccagatat gcaaaatgct
    36481 ctgcgtttgc atgatgaaat cagccagttt accgatcaaa tggtgatgaa tctggcggac
    36541 tgggaaactc ttccctacga cagtttttcg cctcatcagg acattatctc ctcgcgcctt
    36601 tccacccttt accagctacc gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg
    36661 cttatgcagc gcgtttgccc acacagtttt ctccacggtc atgcgctggt gatgaaaaaa
    36721 ggtcagcgcc tgtcacgaga tgcattacga acccaactgg acagcgccgg ttatcgccat
    36781 gttgaccagg tgatggagca cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc
    36841 ccgatgggga gtgagctgcc ttatcgtctt gatttctttg atgatgaaat cgacagcctg
    36901 cgggtgtttg acgtcgacag ccagcgcacg ctggaggaag tagaagcgat caatctgctg
    36961 cccgcgcacg aatttccgac cgataaagcg gcaattgaac tgttccgcag ccagtggcgc
    37021 gataccttcg aagtgaagcg cgatccagaa catatttacc agcaagtgag taaaggcaca
    37081 ttacctgccg ggatcgagta ctggcagcca ttgttcttca gcgaaccact gccgccgctg
    37141 ttcagttatt tccctgccaa taccttgctc gtgaatactg gcgatctgga aaccagtgcc
    37201 gaacgtttcc aggctgacac gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc
    37261 ccgctgttgc caccacaatc gctctggctg cgggtggacg agctcttctc agagctgaaa
    37321 aactggcccc gggtgcagct aaaaactgaa catttaccga caaaagccgc gaatgccaat
    37381 ttaggtttcc agaaactgcc agacctggcc gttcaggcac aacaaaaagc gccgctggat
    37441 gcgctgcgta agttcctcga gactttcgac ggtccggtgg tgttctcggt agaaagtgaa
    37501 ggtcgccgtg aagcgctggg tgaactgctc gcacgaatta aaattgctcc gcaacgcatt
    37561 atgcgtcttg atgaagccag cgaccgtggg cgttatctga tgattggcgc tgccgaacat
    37621 ggttttgtcg atacggtgcg taatctggcg ctgatttgcg aaagcgatct gctcggtgaa
    37681 cgcgttgccc gtcgtcgtca ggattctcgc cgcaccatca accccgatac actgatccgt
    37741 aaccttgcgg aactgcatat tggtcagccg gtggtccatc tggagcacgg cgtcggtcgt
    37801 tatgccggaa tgaccacgct ggaagcgggt ggcattactg gcgagtatct gatgctcacc
    37861 tatgccaacg acgccaaact gtatgttccg gtgtcgtcac tgcatctgat tagccgttac
    37921 gcaggtggcg cggaagaaaa cgccccgctg cataaacttg gcggcgatgc gtggtcacgc
    37981 gcgcggcaga aagcggcgga aaaagtgcgt gatgtggcgg cggaattgct ggatatctac
    38041 gcgcaacgcg ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca gtatcagttg
    38101 ttctgcgaca gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat taatgcggta
    38161 cttagcgaca tgtgtcagcc gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt
    38221 ggtaaaacag aagtggcgat gcgcgcagct ttcctggcag tagataacca caagcaggtg
    38281 gcggtgctgg tgcctaccac ccttctcgcg cagcagcatt acgacaactt ccgcgaccgt
    38341 ttcgccaact ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc caaagagcag
    38401 acgcaaatcc ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa
    38461 ctgctgcaaa gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga tgaagaacac
    38521 cgcttcgggg tgcgtcataa agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg
    38581 acgcttactg caacgccgat cccacgtacg ctgaatatgg caatgagcgg aatgcgtgac
    38641 ctgtcgatta tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag
    38701 tatgacagca tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg aggacaggtt
    38761 tattatctct acaatgatgt ggaaaacatt cagaaagccg ccgaacggct ggcagaactg
    38821 gtgccagaag cgcggatcgc catcggtcac gggcagatgc gcgagcgcga actggaacgg
    38881 gtgatgaatg atttccatca tcaacgtttc aacgtgctgg tttgtacaac cattatcgaa
    38941 accgggatcg acatcccgac agccaacact attatcattg aacgcgcgga tcacttcggt
    39001 ctggcgcagc tgcaccagtt acgcggtcgc gtcggacgtt cgcatcatca ggcatatgca
    39061 tggttgctga caccgcatcc aaaagcgatg actaccgatg cacaaaaacg tcttgaagca
    39121 attgcctcgc tggaagatct cggggcaggt tttgcgctgg caacgcacga tctggagatt
    39181 cgcggcgcgg gtgaactgct tggcgaagaa caaagcggct caatggaaac catcggtttc
    39241 tcgctgtata tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg
    39301 tcgctggaag atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg
    39361 ccagatgatt tcatccctga cgtgaacacg cgtctgtcgt tctacaaacg tattgccagc
    39421 gccaaaacgg aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg
    39481 ctgccagatc cggcgcgtac cctgctggat attgcccgtc tgcgccagca agcgcagaaa
    39541 ctggggatca ggaagctgga aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag
    39601 aatcacgtta atccggcctg gttgattggt ttgctgcaaa aacagccgca gcattaccgc
    39661 cttgatggtc cgacgcgcct gaaatttatt caggatttga gtgagcggaa aacgcgtatc
    39721 gaatgggtac gccagtttat gcgtgaactg gaagagaacg cgatcgctta atggtaaatc
    39781 gggggcgttt ctgcgccccc atctgcaaca tttacaaatt ctttgcactt ccctgcacta
    39841 tccgacactg tcaccatcca taattcaggc ttatctgttt attacaataa ccttatattt
    39901 attatggatt tttggttatg atcaaaacgc gtttttctcg ctggctaacg ttttttacgt
    39961 tcgccgctgc cgtggcgctg gcgctaccgg caaaagccaa cacctggccg ctgccgccag
    40021 cgggcagtcg tctggttggc gaaaacaaat ttcatgtggt ggaaaatgac ggtggttctc
    40081 tggaagccat cgccaaaaaa tacaacgtcg gctttctcgc tctgttacag gctaaccccg
    40141 gcgttgatcc ttacgtaccg cgcgcgggca gcgtgttaac gatcccgttg caaaccctac
    40201 ttccagatgc gccgcgcgaa ggcattgtga tcaacattgc ggagctgcgt ctctattact
    40261 acccgccggg taaaaattcg gtaaccgtgt atccaatagg tattggtcag ttaggtggtg
    40321 acacgctgac accgacaatg gtgaccaccg tttcagacaa acgtgcaaac ccaacctgga
    40381 cgccaacggc aaacatccgc gcccgttata aagcacaggg aattgagttg cctgcggtag
    40441 tgccggctgg accggataac ccaatgggcc atcatgcgat tcgtctggcg gcctatggcg
    40501 gcgtttattt gcttcatggt acgaacgccg atttcggcat tggcatgcgg gtaagttctg
    40561 gctgtattcg tctgcgggat gacgatatca aaacactctt tagccaggtc accccaggca
    40621 ccaaagtgaa tatcatcaac actccgataa aagtctctgc cgaaccaaac ggtgcgcgtc
    40681 tggttgaagt acatcagccg ctgtcagaga agattgatga cgatccgcag ctgctgccaa
    40741 ttacgctgaa tagcgcaatg caatcattta aagatgcagc acaaactgac gctgaagtga
    40801 tgcaacatgt gatggatgtc cgttccggga tgccggtgga tgtccgccgt catcaagtga
    40861 gcccacaaac gctgtaaagc ccggataaaa aaatccccgc gacacggcat gtcgcgggga
    40921 aaaaatcagc agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt
    40981 ccatggaggg tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca
    41041 tccgcttttt gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg
    41101 attgtaccga ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagctg
    41161 gcaaatgaca tggagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata
    41221 gtggccttat gcagatgaat gacgtcgcaa gcaa
//
LOCUS       DBBGLQ010000036        41242 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.036, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000036 DBBGLQ010000000
VERSION     DBBGLQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.036"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(466..939)
                     /locus_tag="ACIYSB_002542"
     CDS             complement(466..939)
                     /locus_tag="ACIYSB_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HGC5429574.1"
                     /translation="MCKLTKEEEYSIISRTMTNIRSLRTYVRELDFEQLLDMQEKLNT
                     VIEERREDAERKAAERKELETKCQQVFEYIVSVGLDPEEWQGPVSAIAGTTETKRKPK
                     GGVRKAKYIFEDENGETRTWSGNGKMPLALRKQVNGDCTLETFLIENPNQHEQSE"
     gene            1247..1993
                     /gene="tia"
                     /locus_tag="ACIYSB_002543"
     CDS             1247..1993
                     /gene="tia"
                     /locus_tag="ACIYSB_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion outer membrane protein Tia"
                     /protein_id="HGC5429575.1"
                     /translation="MKKVIAVSALAMAGIFSAQALADESKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWHSPMGDGGREDTQNRLSVNTLMMNTYYDFRNSSAFTPWVSVGVGYARVHHKAT
                     YTDTSWNKSGEVSDISALHYSGYDNNFAWSIGAGVRYDVTPDIALDLSYRYLDAGKSS
                     LSYKDTEGDKYKSEADVKSHDIMFGVTYHF"
     gene            2346..2963
                     /gene="leoC"
                     /locus_tag="ACIYSB_002544"
     CDS             2346..2963
                     /gene="leoC"
                     /locus_tag="ACIYSB_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin-like protein LeoC"
                     /protein_id="HGC5429576.1"
                     /translation="MDVNRAQCITTKEYFSRLYDDIYHNLQQTTDDISKLHVDNEDGK
                     KQLNVMMEQLQTLQNNFNHKLNYLKQHAEWDRFTVAFFGETNAGKSTIIESLRIFFDE
                     LSRKQLLQNNQNDLQQAEQVLCENLEMLRRDLIQAYSEVANKTRDIRLSAKCLQQIIA
                     NESQSRLQILQQQTHAKVRFTLLATAFGCFIAGAGGMAALLSQIW"
     gene            2974..4689
                     /gene="leoB"
                     /locus_tag="ACIYSB_002545"
     CDS             2974..4689
                     /gene="leoB"
                     /locus_tag="ACIYSB_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin-like protein LeoB"
                     /protein_id="HGC5429577.1"
                     /translation="MSDTWSSVEENPERVFRDTHPEGNSASDILYLLEQNWSRFVSSF
                     ILTERLKNRARRVDGLIIGTGESDFTKGNTHYDLCIDDKVFRLIDVPGIEGNENCYAD
                     LVKEAVAQAHLVVYVNGTNKKPEATTARKIKSYLEYGTQVYPLINVRGYSEAYEFDED
                     RIDLEQQGRAGDALCQTVGVLASVLGPDVLLNGNCVQGLLAFSALAYDDNTQSTTIHP
                     SRDLNLAVYQRGFFDVFPSRQEMLTFSQIDAVAQTIKNKVATFREDIIESNKGKVRET
                     LEQYLQVLEEQLSSHHCFLQKIMPEFEKCRVAFRNATGEFERRIANSRRNRWNTLFND
                     LSNASDNIVEDNFGDNSAISTLIRREYEKRREIMGQVMLEDTEESVKVLQQQTLQAVN
                     RLLEDIHNVEFQHPLSFAHSRSIEFSGEMVPRYDLGVTDFSSMALKVGSYAMSGSAIG
                     SIFPVIGTLVGSALGALVGILMTVAGIFTSKASKIRKAQGKVRDRLEEVRDKSLDSVV
                     DETRLLVAAIEKELESGLLQKVNDMQVALQQPVAIFETQISRITTLKKQLEDMPYGTI
                     QTVQY"
     gene            4661..6394
                     /gene="leoA"
                     /locus_tag="ACIYSB_002546"
     CDS             4661..6394
                     /gene="leoA"
                     /locus_tag="ACIYSB_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin secretory dynamin-like GTPase LeoA"
                     /protein_id="HGC5429578.1"
                     /translation="MEQFKQFSIEKQAVINSLLQLRGMLEMLGEMGINISDDLQKVAS
                     AINAIESDVLRIALLGAFSDGKTSVIAAWLGKVMDDMNISMDESSDQLSIYKPEGLPD
                     QCEIVDTPGLFGDKEREVDGRLVMYEDLTRRYISEAHLIFYVVDATNPLKESHSDIVK
                     WVLRDLNKLSSTIFVINKMDEVTSLTDQALFDEQATIKKANLKGKLQRAADLTAQECE
                     QLNIVCVASNPNGRGLTYWFAKPEHYESRSRINDLKNAATEILKTNVPEVLLVKTGMD
                     VVKDIVIQRVTQASRHLDELNTFVEKNDEDMHRFSYDIKQSRIEVKRLAGELFEELNL
                     MEKQLMSQLRPLDLDDIRPFMDDELGYTEDGVGFKLHLRIKQSVDRFFEQSTAVSQRL
                     SDDITRQLSSSESFLSGLGEGAFRSLGGAFKGVSKISPATLKTTILAARDTIGKLTGY
                     VYKFKPWEATKLAGSIAKWAGPVGAAFTIGSDLWDAYKAHEREQELKEVKASLAKIIK
                     EPFEDIYDVLSSDEKMFAFFAPQIQQMEQVVTELAEKSQAIRDNRQKLSLIQTQLAQL
                     MVPATENHTAR"
     gene            6773..8446
                     /locus_tag="ACIYSB_002547"
     CDS             6773..8446
                     /locus_tag="ACIYSB_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGC5429579.1"
                     /translation="MDLPVTACSATSAGQKYRFYSIIMKINLPFIHWPRICNDKRLVL
                     SVVVFTLSNLLVSSGAHLIYRILFFILALLIIKAMKSITLRLILAFPFTLLTAADISI
                     SLYSWCTFGTTFNDGFAISILQTDPDEVIKMFRMYVVYVIAFIILFLLFFCSAINKTS
                     SLPSEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEHQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARTDNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSADSVLHHDIRNYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGSRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQIFAMLETRRASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMCIWYSPALGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTRPAQPKTPEEVITRWQGKSDVFDANHNVFDYNVLRKNFYKPLE"
     gene            complement(8589..9632)
                     /locus_tag="ACIYSB_002548"
     CDS             complement(8589..9632)
                     /locus_tag="ACIYSB_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGC5429580.1"
                     /translation="MNDRLRFEVNDNQGRFVFPDTWFGPLLGEFEEVLDAYDTDEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKKHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNYSAGPDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQKLLWERIDETLSEEIVQKCRNINGEYVWPWILPF
                     SAAGMKHTSIQHQ"
     gene            complement(10068..11258)
                     /locus_tag="ACIYSB_002549"
     CDS             complement(10068..11258)
                     /locus_tag="ACIYSB_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5429581.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQATDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSRQ
                     ESQG"
     gene            complement(11435..11510)
                     /locus_tag="ACIYSB_002550"
     tRNA            complement(11435..11510)
                     /locus_tag="ACIYSB_002550"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11475..11477),aa:Phe,seq:gaa)
     gene            complement(11617..12192)
                     /gene="yjdC"
                     /locus_tag="ACIYSB_002551"
     CDS             complement(11617..12192)
                     /gene="yjdC"
                     /locus_tag="ACIYSB_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5429582.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(12229..13926)
                     /gene="dsbD"
                     /locus_tag="ACIYSB_002552"
     CDS             complement(12229..13926)
                     /gene="dsbD"
                     /locus_tag="ACIYSB_002552"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGC5429583.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAAPQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRIVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(13902..14240)
                     /gene="cutA"
                     /locus_tag="ACIYSB_002553"
     CDS             complement(13902..14240)
                     /gene="cutA"
                     /locus_tag="ACIYSB_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGC5429584.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(14356..15657)
                     /gene="dcuA"
                     /locus_tag="ACIYSB_002554"
     CDS             complement(14356..15657)
                     /gene="dcuA"
                     /locus_tag="ACIYSB_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGC5429585.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(15775..17211)
                     /gene="aspA"
                     /locus_tag="ACIYSB_002555"
     CDS             complement(15775..17211)
                     /gene="aspA"
                     /locus_tag="ACIYSB_002555"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGC5429586.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17548..18024
                     /gene="fxsA"
                     /locus_tag="ACIYSB_002556"
     CDS             17548..18024
                     /gene="fxsA"
                     /locus_tag="ACIYSB_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGC5429587.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(18040..19296)
                     /gene="yjeH"
                     /locus_tag="ACIYSB_002557"
     CDS             complement(18040..19296)
                     /gene="yjeH"
                     /locus_tag="ACIYSB_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HGC5429588.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            19572..19865
                     /gene="groS"
                     /locus_tag="ACIYSB_002558"
     CDS             19572..19865
                     /gene="groS"
                     /locus_tag="ACIYSB_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGC5429589.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            19909..21555
                     /gene="groL"
                     /locus_tag="ACIYSB_002559"
     CDS             19909..21555
                     /gene="groL"
                     /locus_tag="ACIYSB_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGC5429590.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            21693..22046
                     /gene="yjeI"
                     /locus_tag="ACIYSB_002560"
     CDS             21693..22046
                     /gene="yjeI"
                     /locus_tag="ACIYSB_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGC5429591.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(22249..23118)
                     /gene="yjeJ"
                     /locus_tag="ACIYSB_002561"
     CDS             complement(22249..23118)
                     /gene="yjeJ"
                     /locus_tag="ACIYSB_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGC5429592.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAAHLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(23513..24541)
                     /gene="epmB"
                     /locus_tag="ACIYSB_002562"
     CDS             complement(23513..24541)
                     /gene="epmB"
                     /locus_tag="ACIYSB_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGC5429593.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDAEEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVECFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            24583..25149
                     /gene="efp"
                     /locus_tag="ACIYSB_002563"
     CDS             24583..25149
                     /gene="efp"
                     /locus_tag="ACIYSB_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGC5429594.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            25201..25326
                     /gene="ecnA"
                     /locus_tag="ACIYSB_002564"
     CDS             25201..25326
                     /gene="ecnA"
                     /locus_tag="ACIYSB_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGC5429595.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            25437..25583
                     /gene="ecnB"
                     /locus_tag="ACIYSB_002565"
     CDS             25437..25583
                     /gene="ecnB"
                     /locus_tag="ACIYSB_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGC5429596.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            25759..26076
                     /gene="sugE"
                     /locus_tag="ACIYSB_002566"
     CDS             25759..26076
                     /gene="sugE"
                     /locus_tag="ACIYSB_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGC5429597.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(26073..26606)
                     /gene="blc"
                     /locus_tag="ACIYSB_002567"
     CDS             complement(26073..26606)
                     /gene="blc"
                     /locus_tag="ACIYSB_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGC5429598.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(26695..27828)
                     /gene="ampC"
                     /locus_tag="ACIYSB_002568"
     CDS             complement(26695..27828)
                     /gene="ampC"
                     /locus_tag="ACIYSB_002568"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGC5429599.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ"
     gene            complement(27891..28250)
                     /gene="frdD"
                     /locus_tag="ACIYSB_002569"
     CDS             complement(27891..28250)
                     /gene="frdD"
                     /locus_tag="ACIYSB_002569"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGC5429600.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(28261..28656)
                     /gene="frdC"
                     /locus_tag="ACIYSB_002570"
     CDS             complement(28261..28656)
                     /gene="frdC"
                     /locus_tag="ACIYSB_002570"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGC5429601.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(28667..29401)
                     /gene="frdB"
                     /locus_tag="ACIYSB_002571"
     CDS             complement(28667..29401)
                     /gene="frdB"
                     /locus_tag="ACIYSB_002571"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGC5429602.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(29394..31202)
                     /gene="frdA"
                     /locus_tag="ACIYSB_002572"
     CDS             complement(29394..31202)
                     /gene="frdA"
                     /locus_tag="ACIYSB_002572"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGC5429603.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            31527..32504
                     /gene="epmA"
                     /locus_tag="ACIYSB_002573"
     CDS             31527..32504
                     /gene="epmA"
                     /locus_tag="ACIYSB_002573"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGC5429604.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            32723..34225
                     /gene="yjeM"
                     /locus_tag="ACIYSB_002574"
     CDS             32723..34225
                     /gene="yjeM"
                     /locus_tag="ACIYSB_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGC5429605.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            34277..34591
                     /gene="yjeN"
                     /locus_tag="ACIYSB_002575"
     CDS             34277..34591
                     /gene="yjeN"
                     /locus_tag="ACIYSB_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HGC5429606.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            34588..34902
                     /gene="yjeO"
                     /locus_tag="ACIYSB_002576"
     CDS             34588..34902
                     /gene="yjeO"
                     /locus_tag="ACIYSB_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HGC5429607.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(34931..38254)
                     /gene="mscM"
                     /locus_tag="ACIYSB_002577"
     CDS             complement(34931..38254)
                     /gene="mscM"
                     /locus_tag="ACIYSB_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGC5429608.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(38276..39244)
                     /gene="asd"
                     /locus_tag="ACIYSB_002578"
     CDS             complement(38276..39244)
                     /gene="asd"
                     /locus_tag="ACIYSB_002578"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGC5429609.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(39341..40393)
                     /gene="rsgA"
                     /locus_tag="ACIYSB_002579"
     CDS             complement(39341..40393)
                     /gene="rsgA"
                     /locus_tag="ACIYSB_002579"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGC5429610.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDISDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            40488..41033
                     /gene="orn"
                     /locus_tag="ACIYSB_002580"
     CDS             40488..41033
                     /gene="orn"
                     /locus_tag="ACIYSB_002580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGC5429611.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(41046..>41242)
                     /locus_tag="ACIYSB_002581"
     CDS             complement(41046..>41242)
                     /locus_tag="ACIYSB_002581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429612.1"
                     /translation="LLNFAFVIFAFLRRYGRRIIREIFPSATPQKSFFCVFISSAEII
                     SKTFNLASKSRRRKWIPALT"
BASE COUNT        10237 a         9909 c        10523 g        10573 t
ORIGIN      
        1 cggcaaactc atattgacgc atttttcagg cgagccactg ggagcgcata cgaatgggcg
       61 ccgtctgacg atggttgatt tattttgttg tacaggggca atcgcctgct aggtaccgaa
      121 ggctggctct gaagtactga gtcgggtgat cgcgtctggc cgctggctgc gaattactga
      181 cacgggattg gtggcaggtg atgaaatcct gtcccggaaa tggctccgga ttgattcaag
      241 ttctgacccg ctgtcagccc gcgtggttgc tgctctggct catttcctat cgtcagtttc
      301 ctcatgcccg gctgcgggtt ttcggactga atgataatat ctgacgacag gagtcgctca
      361 tctatcgaag gtaaatctcc ctgaaagtaa tagttctggc agggggaaca gaactgtaat
      421 cattgtaccg taatttgagt tggctacctt tgctacatga aaactttatt cactttgttc
      481 atgttgattg gggttttcaa tgaggaaagt ttccagggta caatctccat taacctgttt
      541 ccgcaaagca agtggcattt taccgttacc agaccacgtc ctggtttcgc cgttctcatc
      601 ctcaaagata tatttcgctt ttctcacacc gcctttgggt ttgcgtttgg tttccgttgt
      661 ccctgcaata gcagaaactg gaccctgcca ttcttccggg tcaagcccca cagaaacgat
      721 gtactcaaat acctgttgac atttagtttc cagttcctta cgttcagccg cttttcgttc
      781 agcatcttca cggcgttctt caataacagt gttgagcttt tcctgcatgt cgagtaattg
      841 ctcaaaatca agttcgcgta catacgtgcg cagtgaacga atatttgtca ttgtccgact
      901 gatgatgctg tattcttctt ctttagtcag tttgcacata atttccctct cttttgcaat
      961 aaacgtgttt tctctggtgg gttgattacc catgtgtctg acattttgta ctgtagtgca
     1021 gttgtaatgc aaacctcccg ggcttttggg acacagcatt tcagtccagg ctccatcatt
     1081 accattttgt tgattcgaaa agaaaaccct catcaatatc gatcgatttt accgatcgat
     1141 atgatcgtat cgatcggttg taaccatttt accctatctt tttcatggtt ttaaaataag
     1201 caaccttatt tgtcgggtag ggtaagaacc aacggagatg attgaaatga aaaaggttat
     1261 tgctgtttca gcacttgcaa tggcaggcat tttttcggcc caggctctgg ctgatgagag
     1321 caaaacaggc ttttatatga ccggtaaagc cggtgcttca gttgtgactc agactgacca
     1381 gcgcttccgt caggactttg gggatgatgt ttataagtat aagggcggtg ataaaaacga
     1441 tactgtattt ggtgcaggcc ttgcggtggg ctatgatttt tatcaacatt acaacgttcc
     1501 agtgcgcacg gaagtggaat tctatggccg cggagctgcg gactcccgtt atacactgga
     1561 tacatggcat tctccgatgg gggatggtgg tcgggaagac acacagaaca ggctcagtgt
     1621 gaataccctg atgatgaaca cgtattatga tttcagaaac agcagtgcat tcactccatg
     1681 ggtatctgtt ggggtgggtt acgcacgggt acatcataag gcgacatata ctgatacttc
     1741 ctggaataaa tctggtgagg taagtgatat ttctgcgtta cattactcgg gatatgataa
     1801 caacttcgca tggagtattg gggcaggtgt tcgctatgac gtaaccccgg atatcgctct
     1861 tgacctcagc tatcgctatc tggatgctgg taaatcctcc ctgtcttaca aggatacaga
     1921 aggggataaa tataaatcag aggctgacgt taaaagtcac gacattatgt ttggggtaac
     1981 ttatcatttc tgatgagaat gtttatccat gcctgcatat tgcaagtgca ggtgtgggta
     2041 ttatccacaa ataaaaagga aagtctggat ggtatatcct cgtccgccaa tgatttttat
     2101 ttgcacgaaa tgtaactgga aaaaaacaat tacccatgtt aactgtataa gacctttttc
     2161 ttcatgcccc cgatgtggat gtaaagaaat tgcggtacgt cgtgcaggtg tcatctcgat
     2221 tttattggca gcatttacgt caggctcttt tcctgaacgt tgacattctg attttcatct
     2281 ttcgataata tttctgttta ttattgttga ataataactg gatatataga agaagcgagg
     2341 tccttatgga tgtgaacagg gcacaatgta taacgacgaa agaatatttc agccgtctgt
     2401 acgatgatat ttatcataat ctgcaacaga caacagatga tatttcaaaa ctacatgttg
     2461 ataatgaaga cgggaaaaaa cagcttaacg taatgatgga gcaactgcag acattacaga
     2521 ataattttaa tcataagctg aattatctga aacagcatgc tgaatgggac agatttactg
     2581 ttgccttttt tggtgaaaca aatgctggta aaagtaccat tattgaatct ttaaggattt
     2641 tttttgatga actctctcgc aaacagcttt tacaaaataa tcagaatgat ttgcaacagg
     2701 cggagcaggt gttgtgcgaa aacctggaaa tgctacgtcg agatttaata caggcatata
     2761 gcgaagttgc caataaaact cgtgacatca gattgtctgc taagtgtttg caacaaatta
     2821 tcgctaatga aagtcagtct cgtttgcaaa ttttacaaca gcaaactcat gctaaagttc
     2881 gtttcacgtt attagcgact gcgtttggct gttttatagc aggtgctggc gggatggcag
     2941 ccttgttgag tcaaatatgg tagggggctg acaatgagtg atacatggtc ttctgtagag
     3001 gagaatccgg aacgggtatt cagggatact cacccggaag gtaactctgc gagtgatatt
     3061 ctgtacttac tggagcaaaa ctggtcgcgt tttgtcagtt cttttattct gacagagaga
     3121 ttaaaaaaca gggcaaggcg cgttgatggt ttaatcattg gtactggtga atccgatttc
     3181 actaaaggta atactcatta tgatttatgt attgacgata aggttttccg gttaattgat
     3241 gtgcctggca ttgaaggcaa tgaaaattgc tatgctgatc tggttaaaga agctgttgcg
     3301 caagcgcacc tggtggttta tgtcaacggt actaataaaa aacccgaagc aacaacagcc
     3361 aggaagatca aatcttatct ggaatatggt actcaggttt atccgttgat caatgtgcgc
     3421 ggttattctg aggcctatga gtttgatgag gatcgtatcg atctcgaaca acagggtagg
     3481 gccggtgacg ctttatgtca gactgttggg gttctcgctt ccgtattagg gccggatgta
     3541 ctgctgaatg ggaactgtgt gcagggattg ctggcgtttt cggcgttggc ttatgacgac
     3601 aatacacaaa gcacgaccat tcatccttca cgtgatctta atctggcggt atatcaacgc
     3661 ggtttttttg atgtatttcc ttctcgtcag gaaatgctga catttagcca gattgatgcc
     3721 gtcgctcaga caattaaaaa taaggtcgca acgtttcgtg aggatattat cgaaagcaat
     3781 aaaggtaaag tccgtgaaac gctggagcaa tacttacagg tacttgaaga gcaattgagc
     3841 agccatcact gctttttgca aaagataatg ccggaatttg aaaaatgtcg ggttgcattc
     3901 agaaatgcga ctggtgaatt tgaacgccgt atcgcgaaca gtcgtcgtaa tcgctggaat
     3961 accctgttta atgacctgag caatgccagt gacaatattg tcgaagataa ttttggtgat
     4021 aacagtgcta tttccacact gatccgtcgt gagtatgaaa agcgccgtga aattatggga
     4081 caagtgatgc tggaggatac cgaagagagt gtaaaggtat tacagcagca aacacttcag
     4141 gcggtaaatc gtctgctgga agatattcac aatgtggaat ttcaacaccc cctcagtttt
     4201 gctcacagcc gcagtattga gtttagtggt gagatggtac cccgttacga tctgggggtg
     4261 actgatttca gttcaatggc tttaaaagtt ggcagttatg ccatgtcagg cagtgcgatt
     4321 ggtagcattt ttcccgttat tggtacgctc gttggctctg cgcttggcgc gctggtcggc
     4381 atactaatga ccgttgcagg catttttacc agtaaagcgt caaaaatacg caaagcacaa
     4441 ggaaaagtac gcgacaggct ggaggaggtg cgggataaat ctctcgacag cgttgtcgat
     4501 gaaacccgcc tcctggttgc tgctattgaa aaagaacttg agagtggtct gctgcaaaaa
     4561 gtcaacgata tgcaggtggc tctgcaacag cctgttgcca ttttcgaaac acaaatcagt
     4621 cgaatcacaa cattaaaaaa acaactggag gacatgcctt atggaacaat tcaaacagtt
     4681 cagtattgaa aaacaggctg tgattaactc gctattacag ttgcgcggaa tgttagaaat
     4741 gctgggagag atggggataa acatcagcga cgatttacaa aaagtcgctt ctgcaattaa
     4801 tgccatcgaa tctgatgtcc tgcgtattgc tctgttgggg gcattctccg atggcaaaac
     4861 cagcgttatc gccgcatggc tgggtaaagt aatggatgat atgaatattt ccatggatga
     4921 gtcctccgat cagttgagta tttacaaacc ggaaggtctg ccagaccagt gtgaaattgt
     4981 tgatacgccc gggctgtttg gtgataaaga gcgcgaggtg gacgggagac tggtgatgta
     5041 tgaagacctg accagacgct atatatctga agcacacctg attttttacg tggttgatgc
     5101 cacgaacccg ctcaaggaga gccacagcga catcgtaaaa tgggtattgc gcgatttgaa
     5161 taaactttct tcaaccatct ttgttatcaa taaaatggat gaagtgacca gcctgacaga
     5221 tcaggcactg tttgacgagc aggcgacaat taaaaaggcg aatctgaaag gcaaattgca
     5281 acgtgcagcg gatcttaccg ctcaggagtg tgagcagcta aatatcgtct gcgtcgcctc
     5341 aaacccaaat ggtcgcggct taacgtactg gtttgctaaa cccgaacatt acgaaagtcg
     5401 ttcgcgcatt aacgatctta aaaatgctgc caccgaaata ctgaaaacta atgtgccaga
     5461 ggtcttgttg gtgaaaacag gtatggacgt ggtgaaagac attgtcattc agcgtgtcac
     5521 ccaggccagc agacaccttg atgaactgaa tacctttgtc gagaagaatg acgaggatat
     5581 gcaccgtttt agttacgaca taaagcaaag ccgcattgag gttaaacggc tggctggaga
     5641 attatttgaa gagctgaacc tgatggaaaa acagctgatg agccaactgc gtccactcga
     5701 tctggacgat atccgcccgt ttatggacga tgagttggga tatacagaag atggggtcgg
     5761 ctttaagctt cacctgcgca ttaagcagtc agttgatcgc tttttcgaac agtcgacagc
     5821 ggtctcgcaa cgcttatctg atgacattac tcgccagttg agctccagcg agagctttct
     5881 aagtggctta ggcgagggag cgtttcgttc tttaggcggg gcctttaagg gggtgtcgaa
     5941 aattagtccg gcgacgctca aaacgacaat tttggccgca cgcgatacga ttggcaaact
     6001 aacgggatat gtctataaat ttaaaccctg ggaggctacc aaactggccg gaagcattgc
     6061 caaatgggca ggtcctgtgg gtgcagcgtt tacgattggt tctgatctat gggatgccta
     6121 taaagcacac gagcgggagc aggaactgaa agaagtaaaa gcctcgctgg cgaaaatcat
     6181 taaagagccg tttgaagata tctatgacgt gctgagctct gatgagaaaa tgttcgcctt
     6241 ttttgcaccg cagatccaac aaatggagca ggttgttact gagctggcag agaaaagtca
     6301 ggctattcgc gacaaccggc agaaactgag tcttattcag acgcagcttg cacaactaat
     6361 ggtacctgcc acagaaaacc atactgccag atagatgcat aatgcagttc cgtacacctg
     6421 tggttgaata acagcagact ctttcatgat tatctggatt gaaagagtgt ctgctgctta
     6481 ttatcggaaa tataacttac ttaacacatg aggcataaat cagtgagatt attgttaagg
     6541 ggcgttgttg gaatgataat ggtctggttg gtcatttatt tcggaatgga attacttttt
     6601 atctccgatc gggttcatcc ggtattaaga ataattgttg tcctgggaat gttattcctg
     6661 atgataatga ttgcgggcgg tatgtgccgt tctcttctgt cacgaaagaa aacatcatga
     6721 cgcacctgtt tacgtcgcta tatttgtggc ggctttatga ttattcagaa taatggattt
     6781 acctgtcacg gcatgttcag caaccagtgc cggacagaaa tatcgctttt actcaattat
     6841 tatgaaaatt aatcttccgt tcatacactg gccgcggatt tgcaatgata agcgccttgt
     6901 gttatcagtg gttgtcttta ccctttctaa tctccttgta tcctcaggag cgcatctgat
     6961 ttaccggata ttattcttta tcctggcact tcttattatt aaggcaatga aatcgattac
     7021 gttgcgtttg attcttgcct tcccgttcac attactgact gctgcagata tcagtatcag
     7081 cctgtattca tggtgtacat ttggcacaac atttaatgat ggttttgcaa tcagtatcct
     7141 tcagacagat cctgatgaag taataaaaat gttcaggatg tatgtcgttt atgttatcgc
     7201 atttattatc ctctttttat tgttcttctg ttcagcaata aataaaacgt cctcattgcc
     7261 atcggaaaag gtcacggtaa ttacattttt attgttaatt acagtaacgt tatattcttc
     7321 cttccagttt gcgttgaaga aacagtatca aataaatgaa gttgatcctt acatagtcgc
     7381 ttcccggttt gccacatata cccctttctt taatctgaac tattttgcgc tggccgcaaa
     7441 ggagcatcag agattaatga cgattgctga taccatacct cactacgatc tggtgattac
     7501 ggataacaac actgacgtat ttgttctggt gattggtgaa tctgcaagga ctgataacat
     7561 gtccatttac gggtattcgc gccctaccac accagagctt cagaaacaga aatcacgact
     7621 gaaactgttc actcaggcaa tcagtggcgc accttacacc gcgctggctg ttccactggc
     7681 gttatctgcc gatagtgtac tgcatcatga tatccgtaat taccctgata acattatcaa
     7741 catggcgaat caggcggggt tcgatacctg gtggctcagt gcgcagtccg ctttccgaca
     7801 gaacggaacg gctgtggcca gtatcgccat gcgggccaga aacagaattt atgtcagagg
     7861 ctatgatgag ttactgctcc cacacctggc tgaagcactg aacagtaatc cggggagcag
     7921 gaaactgatt gttctgcatt tgacgggcag tcatgagcca gtctgcagta actggcccag
     7981 agataaagcc gtgtttaaac cgctggatac agaagaagtc tgctacgata attccattca
     8041 ttataccgac agtttgttgg gacagatttt cgccatgctg gaaacgcgtc gggcatcagt
     8101 catgtatttt tcagatcacg gtctggagta tgacccgaca aaagagcatg cctattttca
     8161 tggcggaata aaacccagcc agcaggctta tcatgttccg atgtgtatct ggtacagccc
     8221 ggctctggga gagcatgtcg atcgccagac ggtgaacagc gttttttcta ctgcgtataa
     8281 tgattatctg attaatgcat ggatgggcgt aaccagacca gctcaaccga aaacgccgga
     8341 ggaggtgatt acccgttggc aggggaaatc agatgttttt gatgcaaacc ataacgtgtt
     8401 tgactataac gtattacgaa agaactttta taagcccctg gaataattcg ttaaagtaat
     8461 ctactacgct attttttcaa aggaaacttt cagaaaatgc ccccagggcg ataacctggg
     8521 gaagggggga ctgtgttatt acaggtggat aaattattgc ttgtttgaaa aatacactaa
     8581 tgcaatatct actgatgctg gatgcttgtg tgtttcattc ctgccgctga aaacggcaat
     8641 atccagggcc agacatattc accgttaata tttctgcatt tctgtacaat ttcctcagaa
     8701 agtgtctcat caattctttc ccacaacagc ttctgctgtc ttaaaatgct ttcgcatatt
     8761 tcgaaatcat cttcctgcat cagcatttcg gcgcatttaa tgatttctgc ccgttcatgc
     8821 agcacagagg aatggttata gagccagttt acaaacagca atgcttccgg atattgtccc
     8881 cataatggtg agtatgttgc ataataatct tcggcagtat ccggcccggc agaatagtta
     8941 tgccatacag caagaggaaa tggatgcaga tttccgcaca gaatttccgc gatgtaagta
     9001 tttgccgcaa atccgcgacg aaatgcagtt gcagctttta ccagttcccc attaagaaaa
     9061 tgcagaagac caagttcata ccagtaaggt gaaaattcat cagcatgttt tttcagtaca
     9121 ctgaaggctc gctcatgatc acctgttcgt aacagctctg aacccaacaa ccagcgggca
     9181 ccctgattat cttcaggatt gtatgccagt attttatcag tgagcataac cgcatcctga
     9241 tgacgctgta agtgcacatt tgcgagaatt gctgcataaa gcgcgcgcaa ataaggccgg
     9301 ttttcgggat gcatccagat gatttcacca ctgaaacttt ccgggatgag acgattacct
     9361 gccgccagtc ctttaagcgc ggcgttcagg gccttacgcg gggcattctg ctccagaaat
     9421 gcatatgcca gatgagcgtg tatatcaata aagtcaggct cccgttgagc aaggcgtctg
     9481 agtttgttga tgtaactcgt ctcgctgatt tcatcagtgt cgtatgcatc gagcacttct
     9541 tcaaactccc ccagtaatgg gccaaaccag gtatccggaa atacgaaacg cccctggtta
     9601 tcgttcactt caaagcgtaa tctgtcgttc attttctgag cctgtaaaac ggatggggac
     9661 ataatctgaa aagcataaaa gtcgtgatgt tgtgtatcaa gtcctgtttt tgtccgggat
     9721 gaatgttgtt tacaatatca tcggcagtgg ttttacatga gaatgaaaat ttatctttat
     9781 ctgcattccg gtgtattgtg aattgtcggc tgaaacggca tattaatcga tttttttagg
     9841 ggaatggtgc tgaaataacg acatgatgat gatttatatt acaggtatca aaattgttag
     9901 cggtccataa cggtgtgatg ttttgagatt gtttacagat gcaatattaa atattttgtc
     9961 aaaaaacaaa aaaatatcta aaaataaagc atgaaaacaa aattcctccg gacctggagg
    10021 ccggaggaga acttgtggat tgagagaaaa tatcagaaca agtgttctta accctgactc
    10081 tcttgcctgg aaagcgaatc aatgtaatct gcgtaccatt gcatcatttc acggcgacca
    10141 tcgagatact gggcatgatt ataggtaccc ctgatggcgt ttttatccac atgagctaac
    10201 tgcatttcaa tccaggcaga attaaagccc tgttcgtgca gaatggtact catggtgtga
    10261 cgaaaaccat gaccggttgc cctgccatcg taaccgatac gtttgagcac catattgatg
    10321 ctggcttcgc tcattggctt gttgatatca caacgtcccg ggaaaaccag ccggtattgt
    10381 ccggagaaag ttttgagctg cagcagaatg tcagtggcct gtcgtgacag tggtacgaca
    10441 tgtttacgtc gcattttcat gcggctttca gggattgtcc acagggcatt atcaaaatca
    10501 atttctgacc attcagcgga ccgtagttca atcgttctga ggcctgtcag aagcagcaga
    10561 tttgtcgcca tgcgggtaac aggactgcca tgatactcag cgagcttccg gagaaaaaca
    10621 gggagctcat ctgctctcag gtgggggaaa tgtgcagctt ttggcgccgc cagcgtactg
    10681 gtcagttcag atgccggatt gacagtggct cgtccggtgg cgatggcata acgaaaaatc
    10741 tgattacagt attgacgcac cttacgaagt ttttccagcg agccacgttt ttctatgcgg
    10801 cgcagaattt ccagcatttc cagcgggagg atatccgtaa cgcttcgttt gcccagatac
    10861 gggaaaatat ctttatccag cgcttccagt acgcttctgg cgtaactttc agaccatgac
    10921 aaagatttat gctggtgcca ttccgtagcg agggcatgga acgtattggt ggcagagatt
    10981 ttctgagtga gtttttcctt tcgcttcttt tcgcccgggt caccaccttc ggagatgatt
    11041 tttcgggcat cgtcgcgttt gattctggcc tcagcaaggg agacgtcggg atagacgccg
    11101 agggccagtt ttttctcttt tcctgctatc cggtatttaa gccgccagta gcgggccccc
    11161 gtggttttta ccagcagata taaaccacgt tcgtcagaga gtttatagtc tttttcttta
    11221 ggtctggctg tttcgacctg tctggctgta agtgccatat gggggctcct gaaactgact
    11281 gaacacaatg agcccccacc ggggccccca atgaacgctt gattgagaga gagttcagta
    11341 gagtctggtg gagttcagaa tgttcagatt gcttgtaacg atgtgattat acttgagttt
    11401 cgtggagttc gggagatgaa tctggaagtg aaagtggtgc ccggactcgg aatcgaacca
    11461 aggacacggg gattttcaat cccctgctct accgactgag ctatccgggc aacggggcgc
    11521 attaaaccgt aatctgcaca tctcgtcaac ctaatttcag taaaagcgat tcaactgctt
    11581 aagattgcgg caaatcgctt cttttctgtg tttctgtcag gtcaatgcac caccctggcg
    11641 gcagcgggcg aagcgcagga tatcttccgc cagccgatgt gcagtgtcga catccgcctg
    11701 gctacgattc accagcattc ggcttaaaca gccttccagc accagttcca tctgctttgc
    11761 taccatcgcc ggatcgtcaa cttccagtgt ggttaacagt tcgtgggtga aatcgtaggc
    11821 cgcgcttttt tgctgatcgg ccagttgatg aatagggtgg ccaggatcgg gataaaacgt
    11881 acaggcagcg ataaatagac agcccggata gcggttgttt ttaacgcact ccgataacgc
    11941 ctgataacgt gccagcagct tttgttcggc ggtttgcgtt tcgtccagca tcagctgacg
    12001 acgccagaca tctatctgtt ggctaagata acgcagcgca tcgtagagga ttgcctcttt
    12061 gtctggccag aagcggcgta gctcgtccag tggataatcc acacgttcag caaccatctc
    12121 cagcgtggtg ttggcaatcc cttgtaattc taataatttc agggcttctc ccagtacatc
    12181 ttcacgttgc acgctatttt cctccgtctt tcccactgca agtgtcgttc acggttggcg
    12241 atcgcgcaaa tgtgcgctga aggtttcagc atccataaag cccgtgacgc gtgcttgtgg
    12301 atgctcctgg ccttgtccgt caaaaaagag aattgtcggt aggccaagga cattaagatg
    12361 ctttaacagc gccacatctt gtgcgtcgtt ggccgtgacg ttggcctgaa gtaagaccgt
    12421 gtctgctaac gctttttgca cctgcgggtc gctgaaggtg tatttctcaa actctttaca
    12481 ggcgacgcac cagtcggcat aaagatctaa catcaccggt ttgcctttgg cttcaacgag
    12541 cgcctgattt aactcatcta ccgttttgat ttgtgtaaag ttgagatgcg tctgagtttg
    12601 cgcggtatgc gtcgcaccaa atgcccaatc ctgaagtggg cgcacgctaa ccaatgccgc
    12661 tgccagcaga ataatttgca caatacgcat ccagccgcgt ttagcctgta ggctggtgat
    12721 aaaggcccag ccaaagaatg cgacacccag cgccgaccac aagcgtaatc cccatacatc
    12781 accaatcact cgctccagca ggaagaccgg cagtgcgagg atcacaaaac caaacgcggt
    12841 tttgacttgt tccatccacg ggccgctttt cggcagcaag cggttaccaa agacggtaat
    12901 tagcatcagc ggcaggccca tgcccaacgc atagagataa agcgtgccgc cgcccagcca
    12961 catgttcccg ctttgggcga tatacagcag aatcgcgcta agcggtgcgg tggtgcatgg
    13021 tgaacagatc agtccggcaa tcgcccccat aacaaacaca ccgccaggtg agccgccctg
    13081 ttggcgattg ctcatcaacg tgagacgtgt ttgcagcgaa gaggggagtt gcagggtaaa
    13141 caagccaaac attgacatcg ccagcaaggt aaagacgatg gcgaggccaa tgagcacgta
    13201 tgggtgctgt agcgccgcct ggaactgtaa ccctgcggcg gcaaccacca gacccagcgc
    13261 cgtgtaggtc agcgccatcc cctgcacata aataaaggtc agcaacaatg ctctggcagt
    13321 ggagagccgc tgtttaccac ccagcacgat gccagaaatc agtgggtaca ttggcagcac
    13381 gcatggcgta aaggcgatac caataccgat caacaacgcc cagagcgcgg aaaagggcaa
    13441 ttgcgcggtg ggctgctctt gctgcggaac agacacaggc tgtggcgctg cgttgttggc
    13501 gaccacttcg cttaacggaa cggttttggt ttctggcgga taacagaaac cggcatcagc
    13561 acagccctgg taggtgacag ttaacgtcgc tcccgcactc gcctggttga tggtgacggg
    13621 aagcgtcagc cgatcgcggt aaatctcgct tttgccgtaa aactcatctt catgccagac
    13681 gccttgcggc agctgcacgt cggcaatttt cgcgtgttcc ggcgtaatgc ggatctgttt
    13741 acggtagagg tagtaaccgt ctttgatctg ccaggtcaga ttaaggtcat gttggttttg
    13801 ctgaaaatca aaagcaaagg cttgatccgc ggggacaaat tgtgaacgtc ccggcgcgtc
    13861 gaataatccg gcaaaaacgg aagtgctgca aagtagcagg atcagcgtaa agatgcgttg
    13921 agccatgaga ggtaatctgt gtctccgtgt gtaacaggta aaaccagaag ttccggggtt
    13981 tgatatggat gatgagactt caggcattcc agcagtgcct gctggtgaga tacggtagtt
    14041 tttaaaatca tctgcacttc gtattcttgc tccagcttac cttcccagta atagagagag
    14101 gtagcgccgg ggatcaaggt cgcgcaggcc gccagttttt ccgccagcac tttggcggct
    14161 aaatcctggg ctgtcgcttc atctggtgcc gtacatagca ccacgacaga cgcggtattc
    14221 gaactttttt catcaagcat aaacacctcg caagaacaga tggaaccgca agagagaggt
    14281 cactatacaa cggacggggg aaggatgtta gtcggcggga aagaaagcgg ggcgtgaacg
    14341 ccccgcgatt gatcattaca gcatgaagct acccagcacg aagccgaagc aaacggccag
    14401 ggcaacaccc agagtacccg ggatgaagaa cggatggttg aagacgaatt taccgatacg
    14461 ggtagtaccc gtgtcatcca tctgtaccgc agcaaccagc gtcgggtagg tcggcagaat
    14521 gaacagacca gacaccgcag cgaaagaagc aacagcggtc agcggtgaaa cgttcagtgc
    14581 cagagccatc ggcatcagtg cttttgcggt tgcagcctga gagtacagca gagcagaagc
    14641 aaagaagaag atgacggcca gcagccacgg atgaccctga atcacttcac cagcggtatc
    14701 tttgatccag tcgatgttgt tggaaacgaa agtatcgccc agccacgcaa cacccaggat
    14761 acaaatacag gcgctcatac ctgctttgaa ggtgctggag ttgaggatgt tgtcggtatc
    14821 gactttacag ataacggtgg tcagagttgc aacgctgagc atgatgatca ggattgcgtt
    14881 ggtggtgttc atcagcggtt tttcaaccag acccatgctt gggctgttga tgattgcata
    14941 gataaccacg ccaactacgc ccagcaggaa cagccagacg gacgtttttg caccggattt
    15001 gatttcaatc tgcttttcac cgcgcagttc aaccaggccc tcttccagac gcttgcgata
    15061 aatcggatcg tcagagagtt tggagttgaa cagcatagtg accaggaagg acatcaccag
    15121 aaccgccagc agggtggacg ggatgaccac ggagagcaga tggaggtagc tgatgccatg
    15181 accttccatc acggaagaca tgtaaaccac tgccgctgag attggcgatg cggtgatcgc
    15241 aatctgcgcg gataccactg cagtagacag cggacggcaa ggtttaacgc cttgttcctt
    15301 cgcaacttca gcgataactg gcagtgtcgc cagagagatg ttgccagtac cagcaaagat
    15361 agtcaggaaa taggtcacga tcggtgcgag gatcgtgatg tatttcgggt tacggcgcag
    15421 cagcttttct gtctgatgaa ccagatagtc cagaccgcca gcaacctgca tggcagaaat
    15481 agcggcgata accgccatga taatggagat gacatcgaac gggatgttac cgggtttaac
    15541 gccaatagcg gcaagaacca gcacccccaa tccgcctgca aaaccaatac ctattccccc
    15601 caatctggcg cccaagaaga tcgccagcaa aactatgatg agttctacaa ctagcatatt
    15661 agccttcctt gttttttaac aagttgatat tagattgtta tttttaagtt actgctcaca
    15721 agaaaaaagg cacgtcatct gacgtgcctt ttttatttgt actaccctgt acgattactg
    15781 ttcgctttca tcagtatagc gttttgcttt gtaagccggg tgcatcagat tctgtacgga
    15841 gaaaatatcg tcaagttccg cttcagtcaa cagaccgcgt tccagaacga cttcacgtac
    15901 actcttaccg gtttcggcac agattttacc cacgatgtca ccgttgtggt gaccgatgaa
    15961 cgggttcagg taagtaacga taccgataga gttgtaaacg taaccttcgc acacttcttt
    16021 gttagcagtg atgccgttaa tgcatttttc cagcaggttg tagcaagcgt tggtcagaat
    16081 gtgaacggat tcgaacatgg cctggccaat gaccggctcc ataacgttca actgcagctg
    16141 acctgcttct gctgccatgg taacagtggt gtcgttaccg atgactttga agcatacctg
    16201 gttaaccact tccggaacaa ccgggtttac tttagctggc atgatggaag agcccgcctg
    16261 cagttccggc aggttgatct cgttcaggcc ggcacgtggg cctgaagaga gcaagcgcag
    16321 gtcgttacag attttggaca tcttcacagc caggcgtttc agcgcgccgt gaaccataac
    16381 ataagcgccg cagtcagagg tcgcttcgat caggtcttca gccggtacgc atgggaagcc
    16441 agtaacttca gccagttttt tcactgccag cggagagtac tctttcggcg tgttcagacc
    16501 agtaccgatt gctgttgcac caaggttaac ttccagcagc agttcagcgg tacgttggat
    16561 gtttttcact tcttctttca gcaggatgct gaaagcgcgg aattcctgac cgagggtcat
    16621 cggtactgcg tcctgcagct gggtacgacc cattttcagg atgtcctgga attcgacagc
    16681 tttacgttca aagccttcac gcagttggtt aatcgcatct accagcttaa tcagggaaga
    16741 gtaaactgcg atacggaaac cggtcgggta ggcgtcgtta gtggactgac atttgttaac
    16801 atggtcgttc gggttcaggt actgatattc acctttttgg tgacccatca gttccagacc
    16861 gatattggcc agcacttcgt tggtgttcat gtttacggaa gtacctgcgc cgccctggta
    16921 gacgtctacc gggaactgat ccatgcattt tccgttgttc aggacttcat cacatgcggc
    16981 aatgatggca ttcgctacac ttttaggaat ggtttgcagc tctttgtttg ccatagctgc
    17041 ggctttttta accattacca taccgcgaac aaattcagga atatcactga ttttgttgtt
    17101 gctgatatag aagttttcaa tcgctctcag agtgtgaaca ccatagtagg catcagctgg
    17161 aacttccctg gtacccaaca gatcttcttc gatacgaatg ttgtttgaca tgtgaacctt
    17221 ctttttcaag ctgccaatga tttgctttaa acacacagaa tatatgtggt ttcgaatgtt
    17281 tttcgaccga cgattatccc ctgcatcgac cgaatacccg agatcatatg ctgcttgagg
    17341 atttctaccg taatctggat cactttaagt gtcggttttt accccttaat tattaatttg
    17401 tgaaatagat caccgctttg ggattactac caaaaatagt tgcgcaaaca tcttgaaatt
    17461 ttgctaatga ccacaatata agctaaacgc gattcgcaac ccattcaggt agccggggtt
    17521 aaccggctgc tattacagga gaaacctttg cgctggttac cttttattgc cattttcctt
    17581 tatgtctata ttgagatttc aatctttatt caggttgccc atgtattggg ggtattgctg
    17641 accctcgtgc tggttatatt cacgtcagtt atcggtatgt cactggtacg taaccagggc
    17701 tttaagaatt tcgtgctgat gcagcaaaaa atggcggcgg gtgaaaaccc agcggcggag
    17761 atgattaaaa gtgtttcgct gatcattgct ggtttgctgc ttttattacc gggctttttt
    17821 accgacttcc tcggtcttct acttttattg ccgccggtgc aaaagcatct gacagtgaag
    17881 ttgatgccgc atttgcgctt ttctcgcatg cctggcggcg gttttagcgc cgggaccggt
    17941 ggcggtaata cttttgatgg tgagtaccag cgaaaggatg atgagcgcga ccgccttgat
    18001 cataaagacg atcgccagga ttaatgtcga aacgccggat tatgtggtta tgccattttc
    18061 cggcgttttt cgttttggca gcaacagcca taaccccgcc agcatgatca gcgcatagag
    18121 acttttccag ccgaccattg ccagtaacag aacgcataac agcccgccaa ccaccgccag
    18181 tagtcgataa cgtccttgca ataatttaca gcctgccagc atgcataaca gataaatcat
    18241 aataaagatg ccattggcat aaataataag agcgtccaga ttgatctcta aagcatgaat
    18301 caccaaagtg ctcaccacac agcagccgag caccgcattg agggcattat tcgggatatg
    18361 gcgagaagag aggcgtgcca ggtagtggtc aggattatgt tgcgcctgcg accagaccag
    18421 gcgggcgaag ctctgtatat aaatgttgag actggcaaag caggccagat agccaatcac
    18481 gcaggcaatc cataacgctc ctacaccgaa caactgcact acaatttttg gaagcgatgc
    18541 tgccgccgcc attttttcac cataggcgtc gaagtgtaag acgactaccg tacagcccca
    18601 gtagactaat cctgccagca gcagaccaat catcaaagca cgaggaaaat cacgctctgg
    18661 atttttaaat tccgaggcga gatgggcaaa tgcctccaga ccgacaaaac accagaacat
    18721 cactgataac gcagcaaata acccggtaag ttcgatatta ccaggtgccg gaaaggggat
    18781 attcgcaggt ttgatatcgc ccgcccacca gatagcgaca atcagcgcga cgataagtcc
    18841 ggcaataacg gtttgtagat tagcactgga actggcaccg cgagtaccga tataccacac
    18901 cagcgccagc gtaccgagtt ctgccaacaa cagttgccag ctatgccagc caaacatcgc
    18961 ctggccgaac ccggcggcaa tttgtagtgc ggcaggcaaa cccacgggaa tgaccgataa
    19021 aaacagccag ccggtgactc gctcaagccg cgaaccaaac gccataccga cgaagtgcgc
    19081 gacgccgcct gcgctgggat agtggcgacc cagaatcgca aacacaatcg caatcgggaa
    19141 cactaagata atcaaaacgg gccacgccca caggctgtta ttgcccgcta ccagcgcagc
    19201 taacgcagga acggcaaaca cgccagtgcc taataatgac gtcgatagca ggccaatgcc
    19261 ctgggccagc cccagttctt gtttgagtcc actcatgggt tgatgtccga ttgcgcccaa
    19321 attttgggca actgcgtaga ttttcgatgg tagcacaatc agattcgctt atgacggcga
    19381 tgaagaaatt gcgatgaaat gtgaggtgaa tcagggtttt cacccgattt tgtgctgatc
    19441 agaatttttt ttctttttcc cccttgaagg ggcgaagcct catccccatt tctctggtca
    19501 ccagccggga aaccacgtaa gctccggcgt cacccataac agatacggac tttctcaaag
    19561 gagagttatc aatgaatatt cgtccattgc atgatcgcgt gatcgtcaag cgtaaagaag
    19621 ttgaaactaa atctgctggc ggcatcgttc tgaccggctc tgcagcggct aaatccaccc
    19681 gcggcgaagt gctggctgtc ggcaatggcc gtatccttga aaatggcgaa gtgaagccgc
    19741 tggatgtgaa agttggcgac atcgttattt tcaacgatgg ctacggtgtg aaatctgaga
    19801 agatcgacaa tgaagaagtg ttgatcatgt ccgaaagcga cattctggca attgttgaag
    19861 cgtaatccgc gcacgacact gaacatacga atttaaggaa taaagataat ggcagctaaa
    19921 gacgtaaaat tcggtaacga cgctcgtgtg aaaatgctgc gcggcgtaaa cgtactggca
    19981 gatgcagtga aagttaccct cggtccaaaa ggccgtaacg tagttctgga taaatctttc
    20041 ggtgcaccga ccatcaccaa agatggtgtt tccgttgctc gtgaaatcga actggaagac
    20101 aagttcgaaa atatgggtgc gcagatggtg aaagaagttg cctctaaagc aaacgacgct
    20161 gcaggcgacg gtaccaccac tgcaaccgta ctggctcagg ctatcatcac tgaaggtctg
    20221 aaagctgttg ctgcgggcat gaacccgatg gacctgaaac gtggtatcga caaagcggtt
    20281 accgctgcag ttgaagaact gaaagcgctg tccgtaccat gctctgactc taaagcgatt
    20341 gctcaggttg gtaccatctc cgctaactcc gacgaaaccg taggtaaact gatcgctgaa
    20401 gcgatggaca aagtcggtaa agaaggcgtt atcaccgttg aagacggtac cggtctgcag
    20461 gacgaactgg acgtggttga aggtatgcag ttcgaccgtg gctacctgtc tccttacttc
    20521 atcaacaagc cggaaactgg cgcagtagaa ctggaaagcc cgttcatcct gctggctgac
    20581 aagaaaatct ccaacatccg cgaaatgctg ccggttctgg aagctgttgc caaagcaggc
    20641 aaaccgctgc tgatcatcgc tgaagatgta gaaggcgaag cgctggcaac tctggttgtt
    20701 aacaccatgc gtggcatcgt gaaagtcgct gcggttaaag caccgggctt cggcgatcgt
    20761 cgtaaagcta tgctgcagga tatcgcaacc ctgactggcg gtaccgtgat ctctgaagag
    20821 atcggtatgg agctggaaaa agcaaccctg gaagacctgg gtcaggctaa acgtgttgtg
    20881 atcaacaaag acaccaccac tatcatcgat ggcgtgggtg aagaagctgc aatccagggc
    20941 cgtgttgctc agatccgtca gcagattgaa gaagcaactt ctgactacga ccgtgaaaaa
    21001 ctgcaggaac gcgtagcgaa actggcaggc ggcgttgcag ttatcaaagt gggtgctgct
    21061 accgaagttg aaatgaaaga gaaaaaagca cgcgttgaag atgccctgca cgcgacccgt
    21121 gctgcggtag aagaaggcgt ggttgctggt ggtggtgttg cgctgatccg cgtagcgtct
    21181 aaactggctg acctgcgtgg tcagaacgaa gaccagaacg tgggtatcaa agttgcactg
    21241 cgtgcaatgg aagctccgct gcgtcagatc gtattgaact gcggcgaaga accgtctgtt
    21301 gttgctaaca ccgttaaagg cggcgacggc aactacggtt acaacgcagc aaccgaagaa
    21361 tacggcaaca tgatcgacat gggtatcctg gatccaacca aagtaactcg ttctgctctg
    21421 cagtacgcag cttctgtggc tggcctgatg atcaccaccg aatgcatggt taccgacctg
    21481 ccgaaaaacg atgcagctga cttaggcgct gctggcggta tgggcggcat gggtggcatg
    21541 ggcggcatga tgtaattgcc ctgcacctcg cagaaataaa caaacccccg ggcagaaatg
    21601 tctgggggtt tttcttttgg tcatctttct agtataagat tcagacacgg acgacgcgag
    21661 tggcgtccag ctcattgatt atggggaata acatgcacgt aaaatactta gcagggattg
    21721 tcggtgccgc gctactgatg gcgggttgta gctccagcaa cgaattgagt gctgccggtc
    21781 agagtgtacg cattgtggac gagcagccag gcgcagagtg ccagctgatt ggtactgcga
    21841 caggtaagca aagcaactgg ctttccgggc aacacggaga agaaggcggt tctatgcgcg
    21901 gcgcagcaaa cgatctgcgc aaccaggcgg ctgcaatggg cggtaacgtg atttatggca
    21961 tcagtagccc gtcgcaggga atgttgtcca gttttgtccc gacggatagc cagattatcg
    22021 gtcaggttta taagtgcccg aactgatgtg atccagaggc tgggtgacaa acgtataatt
    22081 gcctgatgcg cttcgcttat caggcctaca taaggcattg caactgattg aatgcttgag
    22141 aatttgtagg ccggataagg cgtttacgcc gcatccggca tgaccaacga tcacgttatc
    22201 agtcatttca ggctggatgg ctccagcctc gcattagcaa agatgagatt atttcgcctg
    22261 tggtgcagtt ttgctggtgg attgaacacg taagtgatgc agcgcgcgta agcattgatc
    22321 ctgggttagc ggttgggcgt tattcgccgc tacggtgcga acatagacct gacatggcac
    22381 acccgcgagt tttttcagtc gtgggctgaa atcaagcatt cggcgagtga tcgcttctat
    22441 ttctttaccc ggcgttgctt cacgcgtttt caccactgcg ccgctaaatt gttctttgcc
    22501 actttcatct ttaaagcggt aaagcaccgc cagataatgg tcgaacaagt tatgtttcca
    22561 ttccggttgg gaataggtgt catattccag attgccattg tcctggcagt caacgtcgta
    22621 taacggcagg aaatctaaca gtgaggtaat acgtaatgcc agttcacgca tttccgcatt
    22681 attaattgca tgaatgattg ctcgcgccag catttcaatc tgcaactcat tgacgactaa
    22741 ctcacatttg ctaccgtcat gtagcgttaa tacgaaggta agattttcgc cttgattatc
    22801 ggtaagttcc agcgtgttaa cccgacgatt aatatcggca tttttaagct catcaaccag
    22861 aatttctggg atgttttctg ccattttctt aatgacttta tcgcgagctt gctcatactg
    22921 aaggtgtgca gcagcatcca gtttatgctt ttgatgcaga cgactttcca gcgcaatcag
    22981 caggtcgcgc agttccatga cagacataaa gaacaacgat tctttattgc gaggttcttt
    23041 aattttcagc gccagtgcaa tgaaattatt actcttgcga ataacaccgg tattaacacc
    23101 cttgatgctt atggccatgt aggttctccc taaccatttc tcaattaaat aattaatttt
    23161 aatttataag ccagataaat gggcttggta gtaatagttg ttaaaataac ataaatagcc
    23221 gtactcactc tatataaaca gaaaagaaag gttaattatt ggtgttagct atataaaaag
    23281 taacaacagc aatgcatatg aataattaaa ggttatgcaa cgggcaaaga ttaaacttcc
    23341 cagtttaatg acaggttgct gtacttgcgt ttgttgctct tgccggatgc agcgtaaacg
    23401 ccttatccgg cctacagttc attgcaaatc cattcaattt caatgcacga tgtaggcctg
    23461 ataagcgtag cgaatcaggc aattttaatg tttaacttcc ctgtttaatc agttactgct
    23521 ggcgtagctg gagatccagc ggcgttttgc tgggttcgcc gccaatttct cgcgccagtt
    23581 tcggcaccag atatcccgac accagtgtca gcaactcacg cataatctgc cgtgcttcgt
    23641 catcactcac cataaaatgc gccgcgccct gtactttatc gagcacatgc aggtaatacg
    23701 gcattacgcc ggcatcgaac aacgcattac tcaggtttgc cagcgtttgt gcgttatcgt
    23761 tcacatcacg taacagaacg ctctggttca gcaaagtaac gcctacccgg cgcaacttag
    23821 ccatcgcctg acggaatgtt tcatctacct cattggcatg gttgatgtga ttcaccagca
    23881 agatttgcag cgtagaacgg gcaaagcatt caaccagcgc ctcggtgata cgtgccggga
    23941 tcacaatcgg cagacggctg tgaatccgca gacgttttat atgcgggatg gcttccagtt
    24001 gtgtgagcaa ccagtccagc tcgtgatctt tcgccatcag cggatcgccg ccggagaaaa
    24061 tcatctcgtc cagttccgga tgcgcagcaa catactcaag tgcagtttgc cagttacgct
    24121 tgttgccctg attttcggca taggggaagt gacgacggaa gcaatagcgg caatttaccg
    24181 cgcagccgcc tttgaccagc aaaagcgccc ggttgtggta tttatgcaac aaaccaggca
    24241 ctacgctgtg ctgttcttcc agtgggtcgg tggagaatcc gggcgcgatg acaaactcat
    24301 cttgcgaggt aagtacctga cgcaaaagag gatcgtccgg attgcctttc tccatgcgat
    24361 cgataaatga gcggggcaca cgcagggcaa aaagcttttt ggcgctgcgt ccggctaaca
    24421 gtttttcctc cgcgtctata ttcaaaagac gcagaagttc atcaggatcg gtcacaacat
    24481 cggcaagttg cgttaaccaa tcttctctgg atggggtatt tagggttaca atatgcgcca
    24541 ttttgtggct tagctaccaa ttaacaaatt tcagagggcc ttatggcaac gtactatagc
    24601 aacgattttc gtgctggtct taaaatcatg ttagacggcg aaccttacgc ggttgaagcg
    24661 agtgaattcg taaaaccggg taaaggccag gcatttgctc gcgttaaact gcgtcgtctg
    24721 ctgaccggta ctcgcgtaga aaaaaccttc aaatctactg attccgctga aggcgctgat
    24781 gttgtcgata tgaacctgac ttacctgtac aacgacggtg agttctggca cttcatgaac
    24841 aacgaaactt tcgagcagct gtctgctgat gcaaaagcaa ttggtgacaa cgctaaatgg
    24901 ctgctggatc aggcagagtg tatcgtaact ctgtggaatg gtcagccgat ctccgttact
    24961 ccgccgaact tcgttgaact ggaaatcgtt gataccgatc cgggcctgaa aggtgatacc
    25021 gcaggtactg gtggcaaacc ggctaccctg tctactggcg ctgtggttaa agttccgctg
    25081 tttgtacaaa tcggcgaagt catcaaagtg gatacccgct ctggtgaata cgtctctcgc
    25141 gtgaagtaat gcggttgtgg tgcggcctgc aggctgcacc atcacttatt caggtcagag
    25201 atgatgaaac gccttatcgt tcttgttttg cttgccagca cgctgctcac gggatgtaac
    25261 accgctcgcg gtttcggcga agacatcaaa catctcggca actccatctc tcgcgctgcc
    25321 agctaatttt tcttctcttc cgaaaaatca tcagattccc atcatttttg gcgatgttgt
    25381 ctattattaa tttgctatag gcaaacataa ataacattac ctaaaaggaa gacgttatgg
    25441 tgaagaagac aattgcagcg atcttttctg ttctggtgct ttcaacagta ttaactgcct
    25501 gcaacaccac gcgtggcgtt ggtgaagaca tttctgatgg cggtaacgcg atttctggcg
    25561 cagcaacgaa agcgcagcaa taagcaataa cggtacgaca gctgtgtcgt gccgtttgtt
    25621 ttttctgcga tagtcacaaa ggtaatagtt gaaattcccc tgccacctgg caaaatatcc
    25681 gttcaaccat cagctttgca ggacgacctg caaacgcctc ttttcaccgg ggacggcccc
    25741 aattctccgg agcctgatat gtcctggatt atcttagtta ttgctggtct gctggaagtg
    25801 gtatgggccg ttggcctgaa atatacccac ggctttagtc gtttgacgcc gagtgttatt
    25861 actgtgacgg cgatgattgt cagtatggcg ctacttgcct gggcgatgaa atcgttacca
    25921 gtagggacgg cttatgccgt gtggacgggt attggcgcag tcggcgcggc cataaccggc
    25981 attgtgctgc tcggtgagtc cgctaacccg atgcgcctgg cgagtctggc gttaatcgta
    26041 ttggggatta ttggtctgaa actcagcact cactaactac caggctgctg tacccaaata
    26101 aatttactga catcaaaccc ttcccgggtc gcgactgcca gcatctcctg tttcacttcg
    26161 tcagaaatgg ttggcgtgcg ggagagtatc cacaggtagt cgcggtccgg gccgcaaacc
    26221 agcgcatggc ggtattcccg atcgagtgca ataacgttat aaccgccata gaaaggacca
    26281 aagaatgaca ctttcagcgc agcgcgagtt ggtgcgccgg taaagtacgc tttcccttca
    26341 ctctgctgcc acattcctct gtcagggtta tagcctttat taatgacatt caggccgccg
    26401 tcatcacgca ggctgtatgt tgcggtgact ttttccagtc cacgttcaaa gcggtgatca
    26461 aaacgggcaa tctcatacca ggtaccgaga taacgtttgg cgtcgaaatt atttactacg
    26521 gtcacgccac gcggcggcgt aggagaactg caggcaacga ccagaaatgc agctgtcgct
    26581 gcggcaacga gagggagcag gcgcataaat gtttccttac tggttttttt ctaagtgtag
    26641 atgacagcaa ggaaaagcgg agaaaaggtc cgaaaattcg gacccgatgg aattttactg
    26701 tagagcgtta agaatctgcc aggcggcgtc gactctcgct ggattgggat agtttttgtt
    26761 tgccagcatc acgataccca gctctttttc tggaataaac gcgacatagc taccaaatcc
    26821 gccggtcgcc cctgttttat gtacccatga tgcgcgtact gcaggagttg ggggcgtaat
    26881 cgcttttacg gggcgtgctg ccagtgcaat tttattgtca ctgccgttaa tgatgctgtc
    26941 aggatttacc ggccagtcca gcatttccca gcccaggccc tgatacatat cgccggtttg
    27001 ccagtagcga gattgtgcca gttgtatccc ttgttgaagc gttttctcat tgatatcaag
    27061 gggttttaaa ttgctttgca cccagcgggc catatcttca atggtcgact tcacaccata
    27121 agcttcagca tctaacgccc caggcgaaac atgcactgcc ttaccttcgc gatatcccca
    27181 ggcgtaattc ttttcttctg cgggcggtac attaatccac gtatggttga gtttgagtgg
    27241 ctggaagaca cgagtttgca tcgcctgctc aaaactcaaa ccagacggct tcacagccag
    27301 tgcgccgaac aaaccgatac tggagttggc atacagacgt tgtgttcctg gagcccatgc
    27361 aggctgccag ttttgataga agcgcagcaa gtcgcttgag gatttcacct catccggcac
    27421 ctgcaatggc aggccgccag cagtgtaggt tgcgagatgt aatagtgtga tcccattcca
    27481 ctgtttagcg gtaagttcag gccagtattt tgttgtggga tcgcttaact tgatttcccc
    27541 tcgagcaata gcgtcgccac caagcacgcc agtaaatgtt ttgctgaccg aacctaactc
    27601 aaacaacgtt tgctgtgtga cgggctgctt tttggcgatg tccgcatagc cccaggtaaa
    27661 gtaataaggt ttaccctgat aaattaccgc caccgccata cccgggatct tttgttgctc
    27721 tataagcggg gtaattgtgc gatgcacaat atcgttgatt tgttgagggg cagcaaatgt
    27781 ggagcaagag gcggtaatta ataaggcgca gagcgtcgtt ttgaacatag ggtctggttt
    27841 ccatacaaaa cggcccgcca taggcgggcc ggatttacat tggcgatgcg ttagattgta
    27901 acgacaccaa tcagcgtgac aactgtcagg atagcagcca gaccgtagaa aacccatttg
    27961 cccgcaggta cgtggatttt cagatcgtgc atcgcgtggt gcatacggtg taaaccacac
    28021 cacagcggca gaacgatcat caggaacagg aatacgcgac caatgaagct ctgcgcgaac
    28081 gccagaacgc gctcgtagct cagcgcatca cccggaaaca accccagtgg cagcagaata
    28141 cccaccagca ggatcatcac cggcgcaatg atggcgctcc acataccacc ggccccgaag
    28201 aggccccaga ataccggttc gtcagaacgc tttggatttg gattaatcat ctcaggctcc
    28261 ttaccagtac agggcaacaa acaggattac gatggtggca accacagtta ccgcccagag
    28321 acttttgata attggctctg gtcccatttt ttcgtctttt acaatgatat tggccgcttt
    28381 cggtgccagt tcaaaccagg ttttggtgtg cagcagagct gccgccagag tgatcaggtt
    28441 aatgatcacg ataaccgggt tttgtaaaaa gtcgacgaat cccgcccagg cttccgggcc
    28501 atttttcagg gcaaacagcc cgaaaatcag ttcaatgctg aaccacacag ccggaaccgc
    28561 cgtgccttcg cgcagcatgt aaaagcgata aaacggcaat tttttccacc aggtggacgt
    28621 cattggccgt acatacggtt tacgtttagt cgtcatgttg cactccttag cgtggtttca
    28681 gggtcgcgat aagaaagtct ttcgaacttt ctactttgcc ctgctgaatg gccgcagccg
    28741 gatcgacgtg tttcgggcag acttcggagc agtagcccac gaaagtacag ctccatacgc
    28801 cgttctggct gttcaactgc gccatacgct ccttcttacc gtggtcgcgg ctatcttcgt
    28861 tataacgatg cgccagcgta atggcagccg gaccgatgaa ctctgggttc aggccaaact
    28921 gcgggcacgc ggcgtagcac aaaccacagt tgatgcaacc ggagaactgg tgatacttcg
    28981 ccatctgcgc cggggtctgg atgttagtac cctgatccgc ggtgcgggag ttgccgatga
    29041 tgtacggttt gatcgcttcc agactttcga tgaagtgggt catatcgacc accagatcgc
    29101 gttcaatcgg gaagttagct aacgcttcaa ccttcatacc gtcggtgtaa tcacgcagga
    29161 aggttttaca tgccagtttt ggcacgttgt taaccatcat gccgcaggaa ccacaaatcg
    29221 ccatacggca ggaccagcgg tagctcaggt ccggtgccag gttgtctttg atgtagccca
    29281 gcgcatccag taatgaggta gttgcgtcat aaggcacttc atagaatgcg ctatgcggtg
    29341 cggtatcgac ttccgggtta tagcgcacca cctcaatttt caggtttttc atctcagcca
    29401 ttcgccttct ccttcttatt ggctgcttcc gccttatcgg ctgcatccgc ttcgccaccg
    29461 taaacgcgtt tagctggcgg cagcgtagta atcttcacgt cgctgtactc caggcgagtc
    29521 gtgccatcag catcgcggaa ggcgagggtg tgtttgagga agttgacgtc gtcacgctcg
    29581 gtgcaacctt cgtccagacg ctggtgcgcg ccgcgggact ctttacgtgc cattgcggag
    29641 tgcgccatac attcagcaac gttcagaccg tggcccagtt caatggtgta gagcaggtcg
    29701 gtgttgaaca cgctggaagt gtcggtgatg cgcacgcgct tgaagcgttc ctgcagctct
    29761 gccagcttgt cgatggtttt ctgcatcagt tccggcgtac ggtagatacc gcagccttct
    29821 tccatagcca ggcccatttc gtcgcggatc ttcgcccagt tttcgccgcc atcctggtta
    29881 accagatctt tcagacgttg ttcaacgcca gctgcctgcg cttcaattgc cgcttcgttg
    29941 ccattaccgg cagttgctgc acgctctgtc gcttgttcac cggccagacg gccgaagacc
    30001 accagttccg ccagggagtt agaacccaga cggtttgcac cgtgcagacc aacagaggaa
    30061 cattcaccca cggcgaacag acctttaatg cgggtttcac agttctgatc ggtttcgata
    30121 ccgcccatgg tgtagtgtgc ggtcggacgt accggaatcg gttctttaac cggatcgacg
    30181 ccaacgtacg ctttcgccag ttcgcagatg aacggcagac gttcatgcag ttttttctcg
    30241 ccgaggtgac gcaagtcgag ataaaccaca tcgccacgcg gcgtggagat ggtgttgcct
    30301 ttacgccatt cgtgccagaa ggcctgagag actttgtcgc gtggacccag ttccatatat
    30361 ttgtttttcg gctcgcccag cggagtttcc gggcccatgc cgtaatcttg cagataacgg
    30421 tagccatttt tgttgaccag aataccgcct tcaccgcggc aaccttcggt catcaggata
    30481 ccggaacctg gcagaccggt tgggtgatac tgaacgaatt ccatgtcacg cagcggaacg
    30541 ccgtggctta gcgccatacc cataccgtca ccggtaacga tgccgccgtt ggtgttgtaa
    30601 cgataaacgc gacccgcacc gccagtagcc ataacgaccg cgttagcacg gatctgcacc
    30661 agcgtgcctt ccatcatgtt cattgctacc aggccgcgaa catgaccatc atcaaccaga
    30721 atatccagca cgaaatgttc gtcaaaacgc tggatctgcg ggaattgcag agaggtctgg
    30781 aacagcgtgt gcagcatatg gaagccggtc ttatcggcgg cgaaccaggt gcgctcgatt
    30841 ttcatgccgc cgaagcgacg tacgttgacg ctaccatccg ggcgacggct ccatgggcat
    30901 ccccacagtt ccagttgggt catttcggtt gggcagtggt ggacgaaata atccacgaca
    30961 tcctgctcac acaaccagtc gccacccgct actgtatcgt gaaagtgata ttcgaagctg
    31021 tcatgatcct gcgcgacagc ggcggagccc ccttctgcag caacggtatg gctacgcatc
    31081 gggtatactt ttgagattag tgcgattttt gcattcggat ttgcctgcgc ggcagcaatt
    31141 gcagcacgta atcccgcgcc accggcgcct acaatggcaa gatcggcttg aaaggtttgc
    31201 acgacattcc tccagattgt ttttatccca cagccacgta cttcagggta agtacctgaa
    31261 agttacggtc tgcgaacgct attccactgc tcctttatag gtacaacagt atagtctgat
    31321 ggataagtct gaaatttgac gagatcgatt tttttagtgc gcaagagggc taaattatca
    31381 ctgaagatga ttaatttaat tactaaacca tcagatcgtg ctttttttag tcactgccca
    31441 ccgctgtttg attcctgcgt aaaatttgtc tcgccgtcgc gttgcgagta gacttcgtgc
    31501 ccttgtcaaa aactggagat ttaactatga gcgaaacggc atcctggcag ccgagcgcat
    31561 ccattcctaa cttattaaaa cgcgcggcga ttatggcgga gatccgtcgt ttctttgccg
    31621 atcgtggagt gctggaggtg gagacgcctt gtatgagcca ggcgacggta accgatattc
    31681 atttggtccc gtttgagaca cgtttcgttg gccccgggca ttcgcagggg atgaatctct
    31741 ggttaatgac cagcccggaa taccatatga aacgcctgct ggttgccggt tgtgggccgg
    31801 tattccagct gtgccgcagc ttccgtaatg aagagatggg gcgttatcac aaccctgagt
    31861 tcactatgct ggagtggtat cgaccgcact atgatatgta ccggttgatg aacgaggtgg
    31921 acgatctctt acaacaggtg ctggactgcc cggcagcaga aagcctttct tatcaacaag
    31981 ctttcttgcg ttatctggaa attgacccgc tctctgccga caaaacgcaa ctgcgggaag
    32041 tcgcagcgaa actggatttg agcaatgttg ctgataccga agaagaccgc gacacgctgc
    32101 tacaattgct gtttaccttt ggcgtagagc caaatattgg caaagaaaaa ccgacctttg
    32161 tgtaccactt tccagccagc caggcatcac tggcgcaaat cagtaccgaa gatcatcggg
    32221 tcgctgaacg ctttgaggtt tattataaag gtattgagct ggcgaatggt ttccatgaat
    32281 tgacggatgc ccgtgagcag caacaacgct ttgaacaaga taaccgtaag cgcgcggcgc
    32341 gcggtttgcc gcagcacccc attgaccaga atctgattga agccttgaaa gtcggtatgc
    32401 ctgactgttc cggcgtggca ttaggtgttg atcgtctggt gatgttggcg ctgggcgcgg
    32461 agacactggc tgaagtcatc gcctttagcg ttgaccgggc ataattctga aaaattactg
    32521 aattaacagc gaagaatgtc gtgataagcg ccattctgta gcatatgttt tcatttttcc
    32581 gttaaaggcc acaatttaag actggatatt tgatatcatc caggtatcaa ttctgtattg
    32641 gtttttacgc tgcctgctca atgttgcgca gtgcagttca gtgggcacgt gttatacacg
    32701 cgctgaaatg aaggatggtt tcatgcctca cacgataaaa aagatgagtc tgataggact
    32761 catattgatg atctttactt ccgtatttgg atttgccaat agcccatcgg cttattactt
    32821 aatgggttat agtgcgattc ccttttatat attttctgca ttgttattct ttattccatt
    32881 cgccttaatg atggctgaaa tgggagctgc ttatcgcaaa gaagaaggcg gtatctattc
    32941 ctggatgaat aatagtgtcg gaccacgttt tgccttcatt ggtacgttta tgtggttttc
    33001 ctcttatatc atctggatgg tgagtacctc cgcgaaagtt tgggtaccgt tctcaacatt
    33061 cctctatggt agcgacatga cccagcactg gcgtattgcc ggactggagc ctacgcaggt
    33121 ggttggtctg ctggctgtgg catggatgat tctggtcacc gtcgttgctt ctaaggggat
    33181 taataaaatt gcccgcatta ctgcggtggg cggtattgca gtaatgtgtc tgaatttagt
    33241 attgctgtta gtaagcatta ctattttgtt attaaatggt gggcatttcg cgcaggatat
    33301 taatttcctt gcatcaccga acccgggtta tcagtccggt ctggcaatgc tatcgtttgt
    33361 ggtatttgcc atttttgcct atggcggaat tgaagcggtt ggtggtctgg tcgataaaac
    33421 ggaaaatcca gaaaagaact ttgccaaagg tattgttttt gccgctattg ttatttcaat
    33481 cggttattcg ctggcaatat ttttatgggg cgtcagcaca aactggcagc aggtattaag
    33541 taatggttcc gttaacctcg gcaatattac ctatgtgctg atgaagagcc ttgggatgac
    33601 gctgggtaat gcactgcatt tgtcacctga agcgtcattg tcgctgggcg tatggtttgc
    33661 gcgtattact ggactttcga tgttcctcgc ctataccggt gcgttcttta cgctttgcta
    33721 ttcaccgttg aaagccatca tccaggggac gccgaaagca ttgtggccgg aaccgatgac
    33781 gcgcctgaat gcgatgggga tgccgtctat cgccatgtgg atgcagtgcg ggttggttac
    33841 tgtcttcatc ctgctggttt cgtttggtgg cggtaccgca tcggcgttct ttaacaagct
    33901 gacgctgatg gcgaacgtgt ctatgacgct tccttacctg ttcctcgcgc tggctttccc
    33961 gttctttaaa gcacgtcagg atctcgacag accgtttgtg attttcaaaa cgcatttgtc
    34021 ggcaatgatt gcgacagtgg ttgtcgtact ggtggtgaca tttgcgaacg tcttcaccat
    34081 cattcaacct gtggttgaag ccggagactg ggacagcaca ttgtggatga ttggcggccc
    34141 tgtcttcttc tcgctgttag cgatggcgat ttaccagaac tattgcagca gagtggcaaa
    34201 aaatccgcag tgggcggtgg aataacccga tgccctatcg tctacctgac gatagggcgt
    34261 aaagacagga aatgttatgg atgacaccag ccgggacccg gcaataaccg aagatgaaat
    34321 cagggcgctg caattttctg ccggggatgt agcggaaata gagcagacga ttctctcttt
    34381 tgtcgatgcc tgccacaccc gtaaagtggc gatggtggtg ggtagcacca ttaacaccct
    34441 caaagatcgc gatggtaaac ggtggggaaa cctgccggat atttattgcg cttatcttat
    34501 ccgatgtctg gtttttcgcg gggaattagt gggatacggc gatttgtttc gtatgcgcta
    34561 tagcgaaatt aagcgaccgg ttaccttatg agtgcgcgca tgtttgtctt atgctgcatc
    34621 tggtttattg tggcgttcct ctggataacc atcacttccg cgctggacaa agagtggatg
    34681 attgatggac gcgggattaa caacgtctgc gatgtgttga tgtatctcga agaagacgat
    34741 acccgtgatg ttggtgtgat aatgacgtta ccgctctttt tcccttttct ctggttcgct
    34801 ttgtggcgaa aaaaacgcgg ctggtttatg tacgccaccg cgctggctat tttcggctac
    34861 tggctgtggc agttttttct gcgctatcag ttttgtttgt gagccggatt ggttcatccg
    34921 gcacacaaac ttacaaactt cccgcctgac gacctttgcc cgcagacgtc agcgttctcc
    34981 ccgtttgttt accgttgagg ctttccagac gcatctggaa gggcgggaat ggcatatcga
    35041 taccgtgggc atggaagcca gccagaatca gctggtggat ctcatggcgt agcggcatac
    35101 ggtgacccat ctcagcggcg taaatacgca gctcgaaaat ctgaatcccc tgttgcagat
    35161 ccaccaggaa gacttccggt gccgggttgt cgatcaccag cgagcagcga cgcgctgcgg
    35221 tgagcaggat ttccgtcact tcttcgctat tggcatcggc aggggccggt atcgtcaaca
    35281 ccacgcgcgt gaccgagtca gagagcgacc agttgataaa ctgctcggta ataaacgcct
    35341 tgttcggcac gattatctct ttacggtccc agtcgctgat ggtggtggcg cgggtgttaa
    35401 ttttcgtcac gctaccggtg agatcgcgaa ttgtcaccgt atcgccaatg cgaatcggtt
    35461 tttcgaacag gataatcaga ccagagataa agttggcgaa aatttcctgc aaaccaaaac
    35521 cgagaccaac accgagcgcg gcaaccagcc actgcaattt cgaccactca ataccaatca
    35581 ttgagaagcc gaccagcccg ccaatcagca tcagcagata tttggtgatg gtggtgatgg
    35641 cgtaacccgt acccggcgtt aaatccaggt gctgcaaaat cgccagttcc agcagcgcgg
    35701 gcaagttgcg caccagctgc gtggtgatga taaacaccag aatggcaatc agcaccgcac
    35761 cgagggtaat tggctccaga ctttctacgc cctgtaccgt ggaggtgaca tcccacagcg
    35821 aaatattttc gaggaagccg aaagcggaat ggatttctga ccacagcaca atgacagaaa
    35881 gcagggcgat caacatcaaa attgagcgca ccagccgcaa ggattgcgca ctgatggcat
    35941 cgagatcgac ttcgctttca tcgacttcaa ttgctccttc cgggctactg tgatgatgcg
    36001 cttcctcttc gccacgcgca cgttgcgcta acatctctgc ccggcgatgc ttcgcccgat
    36061 caaacgccag cctgcggcgc tggatcagca tccagcggcg gataacgtga taaaccacca
    36121 gtagcaggaa ccagatggca accgaggttt caagcctcgc taacagcgcc tgtgccgttg
    36181 ccagataacc caccgccgac gccagaatgg caaccaacgg cgcgccaatc atcatgttcc
    36241 acagcatatg gttggtaatg ttgtcgccgc tgccctcttt gttgagatac agcggaatcc
    36301 cggccttttt caggctgagg gtgaccaccg ccagcgcacc gcaaatgagg ataaagcaaa
    36361 gccgtcccag cgaaccggag aattcacggt cgtcgaggtt atcgaacatc atcagcgcca
    36421 taatcagcgg cacaataagc ccgatgctca tcaggtagta gcgcatccca cgggaaacac
    36481 gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt cgggcgggca aaggtggcgc
    36541 aaatcatcac tacccacagc agcggcacgg tggccgttac accatcgcca atcgcgaccg
    36601 ccagcggata aggccacgcc tcgcgcaagc cgtaacccag cgtcatccac agcaccggta
    36661 acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa cgtcagccag aagtgatcct
    36721 gagtcacttt gccaactttc gccgccgaac gttcaagaaa acgggtgaaa tagcggcgcg
    36781 agtaaatact gcaaccgacc agaatcaacg cgccaaacag cggcaaaatt gtctctttgc
    36841 tggtcagcat catcacactg gctttgccca actgactgaa ggtgtccagc gaaatgagac
    36901 gacgcagatc ctgggcgatt tccagcggcc aggcgatggt catcgggcgc acgtcagagg
    36961 tccagaacag atagcggtgc gttgcttcgt tcacctcttt cagcgcatcc tccagttgcc
    37021 cgttggagac tttcagcttg gtcagttcca atagtagcgt gtcgccaccc tgcaacaatg
    37081 agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag aatacggttt tgctcggcag
    37141 tcagcggctg accgtcggcc tgatgaattt gccgtagcag cggctgttta ttaagcagat
    37201 cctcataacg taaccgttgc acacgcaact gcgccatttc ggtatcaagc tgttgtggtt
    37261 tcggcatttc cggcagccgt gccacctgcg cccgcagcgc ttcgccgagc agattggacg
    37321 atcccagcca ttgcgactgt tcacgcagcg tattcaacgc ctgccggacc tgtaacgtct
    37381 ggctggcagc ctgacgctgt tgcgaggcaa cgagatccat ccgctgcgcc tgttgattca
    37441 aagccgccga tagttcgcgg ttaattttga attgcgcgac gatatctttc ggcaaatcgg
    37501 cgctgttttc tgccagcaat tcggtacttt ccagcgcccg ctccgcctca agctgacgtt
    37561 ggctgtttaa ttgattacgc aaggcctgca aatacgcatc cagttgctgg ctctcttttt
    37621 ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt attggcagac agctgcgcca
    37681 gctccagttc atcaacgagc gccttaagac gtgcagagtc agactgcaac gcgaaatttt
    37741 gtgcctgatt gagcggagta ttgccggtaa gcgttcccag gcggcgctcg atctcattta
    37801 actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt cagcgaatcg gcaatctcgc
    37861 gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc cagcaactgg ctgctgacct
    37921 ggagaatttc ctgattcagc gcgtcggtag acattcccgg cgacacgctg cgcggctcgt
    37981 cacgcatgtt gtttaattgt gcgcgcagag tagcggagag tttcggataa ttatcgataa
    38041 cttgctgata ttgtttgatg cgctcaaggg aaccttttcg ttcctcaagc gcatttaagg
    38101 cagactggag cgcctctacg acttccggct gtgcgggttt cgccgctttt gcctgctcca
    38161 gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc gtacgccccc caactgaggc
    38221 accaggccat cagaaaagtg ataatcaggc gcacgtcagc gtttcctttg atggattaga
    38281 cctggtcttt tttgtcgtca accaatgggc tggcgtcgtg ttctgcttcg atctcttcag
    38341 caggaagcgg ggcaggttca gcgtctggcg taacaaaggt ttcggtagat actgccagcg
    38401 gctggccaat tttcgtgaca gacaggcttt ccagttgctc aaccagattc actttacccg
    38461 gtgcaaacag gttgataacg gtggaaccga gtttaaagcg acccatttcc tggcctttca
    38521 gcagtgccac agaaccgtcg ttttccccgg caggccaggt ccagcgcttg atgatacctt
    38581 cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat gctgccaaca atcgtcgctc
    38641 cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa aaggcaaatc acgcgttcgt
    38701 tacgggcaaa cagattcggc acgttctgag ccgtgagatg gttaacggag aagagatcgc
    38761 ccggcacgta gatcatctca cgcagaatac cgttgcacgg catgtgtacg cggtggtagt
    38821 cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg gaacaggtcc gccatcagat
    38881 agttgcctgc cagcagggct tcgaggctgt agttgtggcc tttggcttgc aggattttat
    38941 cttcttcgat tttacccaac tggctgataa cgccatcggc aggcatgacc agtacattcg
    39001 gatcggtatc gattgggcgt acttcgtcac gcagcggacg gacaaagaat tcgttaaagg
    39061 tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat gtcgaccttg tagtatttaa
    39121 cgaacagatc gataaccagt tttgtcagcc atcctgcccg cttgcttgcg ccccaacccg
    39181 ccaggcgagt aagccatagt ttcggcagaa tgtactgtag cgaaagttta aatgaattta
    39241 acaaggtagc ctccaggcca ttgttttgtc gttcctgatc cggcctacat gccggatcct
    39301 gaaaaaaagg ggacgattct aacgacggtt agcttaattg tcagtcatcc gtatcagaaa
    39361 agtttttacg cgtttttacc tgcgccatgc tttccagaat acggtgatag ttttcgaaac
    39421 gggtttccgc gattttccct tcctcaaccg cttcccggat agcgcagccc ggatcggtat
    39481 cgtgtttgca atcgcgatat ttacacagac ctaaatagtc atggaattcg acaaagccct
    39541 gagtgatttg ttccggctcc aggtgccaga ggccgaactc acgcactcct ggggagtcaa
    39601 tcacatcacc gccgtgcggg aagtgataca gccgagcggc ggtcgtggtg tgctgaccga
    39661 gccccgagtt gtcagagata tcgttggtca ggatctcttt ttgcagcccc agcaacgcat
    39721 tcagcaggct ggatttgccg acgccagact gcccggcaaa aatgctgatg cgcccggtca
    39781 acgcctcttc cagcggtttt agcccatcct gagtatggct ggaaaccatc aatacgcgat
    39841 aaccgatatt gcggtagata tccatctgct cgttgacgaa cgccatgcct tcgtcgtcca
    39901 gcaggtctat cttgttgagc acaataatcg gctcaatctg caaggtttcg caggccacca
    39961 ggtaacggtc gataatattg agcgacagct ccggcaaaat ggcggagaca atgacaatct
    40021 ggtcgatgtt ggcagcaata ggttttacgc cgtcgtagaa atccggacgc gtcaacaccg
    40081 aggtacgctc atgcaccgct tccacgatcc ctttgacatt tacgccttcc gccgccggtt
    40141 tacccggacg ccagactacg cggtcgccgg ttaccagcga acggatggta cggcgaatat
    40201 tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc gtgcatacca aagcggctga
    40261 tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc gtagtcgggc ttctccttag
    40321 acgttttaag acgacgctgg tgattggcgt tcacgcggcg ctgctggcct ttggagagtt
    40381 tatttttact caatcgtaca gactcctggt cgcccctgat gggcaaaaca tctatgatac
    40441 acgcaattgt ggatcaatat agtcactgtg aatgggtgga aaatagcatg agtgccaatg
    40501 aaaacaacct gatttggatc gatcttgaga tgaccggtct ggatcccgag cgcgatcgca
    40561 ttattgagat tgccacgctg gtgaccgatg ccaacctgaa tattctggca gaagggccga
    40621 ccattgcagt acaccagtct gatgaacagc tggcgctgat ggatgactgg aacgtgcgca
    40681 cccataccgc cagcgggctg gtagagcgcg tgaaagcgag cacgatgggc gatcgggaag
    40741 ctgaactggc aacgctcgaa tttttaaaac agtgggtgcc tgcgggaaaa tcgccgattt
    40801 gcggtaacag catcggtcag gaccgtcgtt tcctgtttaa atacatgccg gagctggaag
    40861 cctacttcca ctaccgttat ctcgatgtca gcaccctgaa agagctggcg cgccgctgga
    40921 agccggaaat tctggatggt tttaccaagc aggggacgca tcaggcgatg gatgatatcc
    40981 gtgaatcggt ggcggagctg gcttactacc gcgagcattt tatcaagctg taaaattttt
    41041 cctggtcacg taagcgccgg aatccacttg cggcggcgtg attttgacgc taaattgaac
    41101 gtcttgctga taatttcagc acttgagata aaaacgcaaa aaaaactttt ttggggggtt
    41161 gcagagggaa agatttctcg tataatgcgc ctcccgtaac gacgcagaaa tgcgaaaatt
    41221 acgaaagcaa aattaagtag ta
//
LOCUS       DBBGLQ010000037        41214 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.037, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000037 DBBGLQ010000000
VERSION     DBBGLQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.037"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(294..2327)
                     /gene="betT"
                     /locus_tag="ACIYSB_002582"
     CDS             complement(294..2327)
                     /gene="betT"
                     /locus_tag="ACIYSB_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGC5429613.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2456..3043
                     /gene="betI"
                     /locus_tag="ACIYSB_002583"
     CDS             2456..3043
                     /gene="betI"
                     /locus_tag="ACIYSB_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGC5429614.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKTRANSLTRHFITQHLPTD"
     gene            3057..4529
                     /gene="betB"
                     /locus_tag="ACIYSB_002584"
     CDS             3057..4529
                     /gene="betB"
                     /locus_tag="ACIYSB_002584"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGC5429615.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4543..6213
                     /gene="betA"
                     /locus_tag="ACIYSB_002585"
     CDS             4543..6213
                     /gene="betA"
                     /locus_tag="ACIYSB_002585"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGC5429616.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6426..7094
                     /gene="ykgH"
                     /locus_tag="ACIYSB_002586"
     CDS             6426..7094
                     /gene="ykgH"
                     /locus_tag="ACIYSB_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HGC5429617.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIR
                     LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(7337..8032)
                     /gene="ykgG"
                     /locus_tag="ACIYSB_002587"
     CDS             complement(7337..8032)
                     /gene="ykgG"
                     /locus_tag="ACIYSB_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGC5429618.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     EYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(8025..9452)
                     /gene="ykgF"
                     /locus_tag="ACIYSB_002588"
     CDS             complement(8025..9452)
                     /gene="ykgF"
                     /locus_tag="ACIYSB_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGC5429619.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9463..10182)
                     /gene="ykgE"
                     /locus_tag="ACIYSB_002589"
     CDS             complement(9463..10182)
                     /gene="ykgE"
                     /locus_tag="ACIYSB_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HGC5429620.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10709..11563)
                     /gene="rclR"
                     /locus_tag="ACIYSB_002590"
     CDS             complement(10709..11563)
                     /gene="rclR"
                     /locus_tag="ACIYSB_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGC5429621.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11789..13114
                     /gene="rclA"
                     /locus_tag="ACIYSB_002591"
     CDS             11789..13114
                     /gene="rclA"
                     /locus_tag="ACIYSB_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HGC5429622.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRMLGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            13223..13459
                     /gene="rclB"
                     /locus_tag="ACIYSB_002592"
     CDS             13223..13459
                     /gene="rclB"
                     /locus_tag="ACIYSB_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGC5429623.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13471..14064
                     /gene="rclC"
                     /locus_tag="ACIYSB_002593"
     CDS             13471..14064
                     /gene="rclC"
                     /locus_tag="ACIYSB_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGC5429624.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(14223..14351)
                     /locus_tag="ACIYSB_002594"
                     /pseudo
     CDS             complement(14223..14351)
                     /locus_tag="ACIYSB_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            14654..15505
                     /locus_tag="ACIYSB_002595"
     CDS             14654..15505
                     /locus_tag="ACIYSB_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5429625.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLMRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNGSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPV"
     gene            complement(15645..19901)
                     /gene="fdeC"
                     /locus_tag="ACIYSB_002596"
     CDS             complement(15645..19901)
                     /gene="fdeC"
                     /locus_tag="ACIYSB_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGC5429626.1"
                     /translation="MSHYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNTISGLSLSASLTGTASEGATVSSWTEKGDCSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKMTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQATLEVDGSTPKVANDNDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSDVTLTASSENLVLDPKGTAKTNEQGQA
                     VFTGSTTIAATYTLTAKVEQANGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATMTVTLMAGVNPVGGSMWVDIEAPEGVTEKDYQFLPSKADHFSGGKITRTFSTSK
                     PGVYTFTFNALTYGGYEMTPVKVTINAVAAETENGEEEMP"
     gene            20453..20542
                     /locus_tag="ACIYSB_002597"
                     /pseudo
     CDS             20453..20542
                     /locus_tag="ACIYSB_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21016..21117
                     /gene="ykgR"
                     /locus_tag="ACIYSB_002598"
     CDS             21016..21117
                     /gene="ykgR"
                     /locus_tag="ACIYSB_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HGC5429627.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            21481..21744
                     /gene="ykgM"
                     /locus_tag="ACIYSB_002599"
     CDS             21481..21744
                     /gene="ykgM"
                     /locus_tag="ACIYSB_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGC5429628.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            21744..21884
                     /gene="ykgO"
                     /locus_tag="ACIYSB_002600"
     CDS             21744..21884
                     /gene="ykgO"
                     /locus_tag="ACIYSB_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGC5429629.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(21919..22146)
                     /gene="ykgL"
                     /locus_tag="ACIYSB_002601"
     CDS             complement(21919..22146)
                     /gene="ykgL"
                     /locus_tag="ACIYSB_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HGC5429630.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            22970..23512
                     /gene="ecpR"
                     /locus_tag="ACIYSB_002602"
                     /gene_synonym="matA"
     CDS             22970..23512
                     /gene="ecpR"
                     /locus_tag="ACIYSB_002602"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGC5429631.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELVYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            23587..24174
                     /gene="ecpA"
                     /locus_tag="ACIYSB_002603"
                     /gene_synonym="matB"
     CDS             23587..24174
                     /gene="ecpA"
                     /locus_tag="ACIYSB_002603"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGC5429632.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            24232..24900
                     /gene="ecpB"
                     /locus_tag="ACIYSB_002604"
                     /gene_synonym="yagY"
     CDS             24232..24900
                     /gene="ecpB"
                     /locus_tag="ACIYSB_002604"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGC5429633.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            24926..27451
                     /gene="ecpC"
                     /locus_tag="ACIYSB_002605"
                     /gene_synonym="yagX"
     CDS             24926..27451
                     /gene="ecpC"
                     /locus_tag="ACIYSB_002605"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGC5429634.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSRLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            27441..29084
                     /gene="ecpD"
                     /locus_tag="ACIYSB_002606"
     CDS             27441..29084
                     /gene="ecpD"
                     /locus_tag="ACIYSB_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGC5429635.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            29053..29763
                     /gene="ecpE"
                     /locus_tag="ACIYSB_002607"
     CDS             29053..29763
                     /gene="ecpE"
                     /locus_tag="ACIYSB_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGC5429636.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNHTRRSPTGGVVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            30076..30405
                     /gene="ykgJ"
                     /locus_tag="ACIYSB_002608"
     CDS             30076..30405
                     /gene="ykgJ"
                     /locus_tag="ACIYSB_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGC5429637.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(30653..31267)
                     /gene="yagU"
                     /locus_tag="ACIYSB_002609"
     CDS             complement(30653..31267)
                     /gene="yagU"
                     /locus_tag="ACIYSB_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HGC5429638.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFIHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            31685..32374
                     /gene="paoA"
                     /locus_tag="ACIYSB_002610"
     CDS             31685..32374
                     /gene="paoA"
                     /locus_tag="ACIYSB_002610"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HGC5429639.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDTLRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            32371..33327
                     /gene="paoB"
                     /locus_tag="ACIYSB_002611"
     CDS             32371..33327
                     /gene="paoB"
                     /locus_tag="ACIYSB_002611"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HGC5429640.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            33324..35522
                     /gene="paoC"
                     /locus_tag="ACIYSB_002612"
     CDS             33324..35522
                     /gene="paoC"
                     /locus_tag="ACIYSB_002612"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGC5429641.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            35532..36488
                     /gene="paoD"
                     /locus_tag="ACIYSB_002613"
     CDS             35532..36488
                     /gene="paoD"
                     /locus_tag="ACIYSB_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HGC5429642.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPVGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            36539..36877
                     /locus_tag="ACIYSB_002614"
                     /pseudo
     CDS             36539..36877
                     /locus_tag="ACIYSB_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            37215..38534
                     /locus_tag="ACIYSB_002615"
     CDS             37215..38534
                     /locus_tag="ACIYSB_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5429643.1"
                     /translation="MTFTITQDRQLASLTVTKEEKEILFAVKSKAGGGLFIRVRYGSD
                     NKSWIYRYRIAQKPAKLTLGSYPAMGLAQARKAHADAIELVRKGIDPRHIRKNEKQKN
                     EQMIIYSELWKDWIEFRKTSKPISTRTLADYEGTYHRHLEKALGNLRIDELTKSVIFE
                     HLSNVRKTSSEAVRKGLIILNMTLDHANLQGMLEHNPARLLKPAMFGASMNKPRERWL
                     PENELRMLWKALDDATVGGGSIASGGYGVSSSVVLSHSVANALRLIIFTGVRRSEAAQ
                     MRWDQINGERWTIPATKNGKSHIVTLHPYMLSLIKTQREFTEGAYVFGSTSKPGFPIT
                     NDALTRALERVRSKYLAELTPFSPHDLRRSVATGCAEYLDAPERLIELLLNHVPKDRL
                     IRTYQVGQQAEKLKNLFIEWGNFIMNNVITVQKNAPDNVIHIKFGNR"
     gene            complement(38627..39475)
                     /locus_tag="ACIYSB_002616"
     CDS             complement(38627..39475)
                     /locus_tag="ACIYSB_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGC5429644.1"
                     /translation="MSDITVSRPEVVNGHTDVIGSTSIRHVVTVRYAALQQAEMLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPEMAMKAITRNLDREIWRDLMQRS
                     GILSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGVHLITGQQTDRVADLERMLHLFSGNPIPDNR
                     ENIIIRLDEHIQSVQGKTCYEDEMFSIRYFKKGSAHITFRKPELIDRLNDIIAKYYPE
                     IIPYNI"
     gene            complement(39560..39757)
                     /locus_tag="ACIYSB_002617"
     CDS             complement(39560..39757)
                     /locus_tag="ACIYSB_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGC5429645.1"
                     /translation="MKSLTMETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQLQLLQQNR"
     gene            complement(39769..40257)
                     /locus_tag="ACIYSB_002618"
     CDS             complement(39769..40257)
                     /locus_tag="ACIYSB_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGC5429646.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDASDLASTDRMPPFAALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWPYPVLRLKRLGLSRTFRRLVVILIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(40254..40631)
                     /locus_tag="ACIYSB_002619"
     CDS             complement(40254..40631)
                     /locus_tag="ACIYSB_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGC5429647.1"
                     /translation="MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPGAKQ"
     gene            complement(40678..41052)
                     /locus_tag="ACIYSB_002620"
     CDS             complement(40678..41052)
                     /locus_tag="ACIYSB_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGC5429648.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
BASE COUNT        10602 a        10485 c        10068 g        10059 t
ORIGIN      
        1 cttaaataac aaatggttgc gttaatttca gttgatttac gatacacatt tctcatgaat
       61 aatgcgtcat ttcaaatgat cagcaatatt tacattaaat aagactaaac ttattgaaca
      121 ttcgcaacag cccaaatgta ggttttgggc tgcaatagtt cttttctttt ttattatctg
      181 ttgtctggta atcatcaata gatactaagg gcttaaccgt atagatataa cgtatcaaga
      241 ggtaggagaa acagcgccat atgcagacat ggcgcggttt tatgcaataa caatcacgcg
      301 tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatgaataaa gttaaggtgc
      361 cgctcgtact ggtcaagaat atcggtgatc acctgctctt tgctgtagtc catcaggtcg
      421 ttgccctggc tgccttctaa caggaaggtt tccagccggt agtaggtcga tttaccgctg
      481 cgtgcgcggt aggtaaagcc cggcaccgaa tattgctgcg gccaaatctg atagacaaag
      541 ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgctg tccctcttcc
      601 ggtggcaggc tttttagctc cacgtacgcg ccgcgcaacc gcaactcctg cgccacttct
      661 tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt gcccggataa
      721 ttcatcaggc gcgagagacg ttttttccag ctcaggcgat cctgaagccc cagcggtcgc
      781 ggtgcggtat cgcggttggc actttcacgg cggtaatctt ctaccttcag agatttatac
      841 aaccccgcca tcacgaagaa gatcacaaag ctgaacggca gccccataat caccgtggtg
      901 ttttgcagcg cggatatccc gttagtcatc agcatgccga gcgtcagcag gccaatcgcc
      961 accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt aagctgcgag
     1021 gtgaaattcc ccagcaccag cgccccggag tccgccgagg tcacataaaa cagcaggcca
     1081 gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag caggctgtag
     1141 aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc gtggatgatt
     1201 tcatacagcg cgctattgcc gaacaccgag agccataaca gcgtgaaggt aaacggaata
     1261 atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac gcgagatacg cgccaggaac
     1321 aagccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt ccagttattc
     1381 atccactcaa ccggacggtc gaaggcaaaa ctgttgagcg tcatgcccat aaagcgattc
     1441 acatagtcgc caacattcag caccagtgca ttaagcagga acgaagtgtc gcccataaac
     1501 aatacgaaca ggatcaatcc cagcgccagc gcgacattaa gctccgataa cacgcgaatg
     1561 cccttatcga caccggaggt gacagagatc gtggcgatta tcaccgacaa ggcgatcagt
     1621 gccgctttcg ccgccatcga atcgggaata tcaaacagta cgctcaagcc atagttaagc
     1681 tgcaccacac cgataccgag cgtagtggca ataccgaaga tagtgccgat caccgctgca
     1741 atatccactg agtgacctat cggcccgtta atccgtttac cgaagatcgg gtacagcgcc
     1801 gagcggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc catgcccatc
     1861 agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac catcgcctga
     1921 cgcgcggcct caatcgtctg tcccgcgcct tccggcggct gcatatactg cgttaccggt
     1981 tcggctacgg agaagaacat caggtcgata ccgatcccgg cagcaaacag catcgccgcc
     2041 caactcagca ggctgaattc cggtttggat tgttctggcc cgagcttcac cgaaccaaaa
     2101 cgcgaacaag cgatacagac cacaaagaca atatagagcg ttgccgccag cagatagtac
     2161 caaccgaagg ttttagaaac ccagtccagc gtgcggccaa tccacagggc cgagaagtcg
     2221 cgaaacagga tcgttgtcag ggaaaacaac aaaatcagtc cggcggaggt gtaaaacacc
     2281 accggattga ttttgtcctt ttccctgctg tgtgaaaggt ctgtcatcca gtatccccac
     2341 tgttattgtt actatttaaa atcaaattcg taacaattaa gacacatttt atattgaacg
     2401 tccaatcaat aaccgcttta atagataaac accgctgatg aatggagtgg cgaaaatgcc
     2461 caaattgggg atgcagtcga tccggcgcag acaactgatc gacgccacac tggaagcaat
     2521 aaatgaagtg ggcatgcacg atgcaacgat cgcgcagatc gcccgccgtg caggcgtttc
     2581 tacggggatc atcagccact atttcaggga caaaaatggt ctgctggaag caaccatgcg
     2641 cgatatcacc agtcagctgc gtgacgcggt tttgaatcga ttacatgcac ttccgcaggg
     2701 cagtgcagag cagcgattac aggcgattgt tggcggaaac ttcgatgaaa cgcaggtgag
     2761 cagtgcggcg atgaaagcct ggctggcgtt ctgggccagc agtatgcatc agccgatgct
     2821 ctatcgttta cagcaggtca gcagccgccg cttgctgtcg aatctggtga gcgagtttcg
     2881 tcgcgaattg ccgcgcgaac aggcacagga agcgggctac ggcctggccg cgctgattga
     2941 tggattatgg ctgcgcgcgg ctctgagcgg caaaccgctg gataaaaccc gcgctaattc
     3001 cctgacccgc cactttatca ctcagcatct acccaccgat taaccgagga gacgtgatgt
     3061 cccgaatggc agaacagcag ctttatatac atggtggtta tacctccgcc accagcggtc
     3121 gcaccttcga gaccattaac ccggccaacg gtaacgtgct ggcgaccgtg caggccgccg
     3181 ggcgcgagga tgtcgatcgc gccgtgaaaa gcgcccagca ggggcaaaaa atctgggcgt
     3241 cgatgaccgc catggagcgc tcgcgtattc tgcgtcgggc cgttgatatt ctgcgtgaac
     3301 gcaatgacga actcgcaaaa ctggaaaccc tcgacaccgg aaaagcatat tcggaaacct
     3361 caaccgtcga tatcgttacc ggtgcggacg tgctggagta ctacgccggg ctgatcccgg
     3421 cgctggaagg cagccagatc ccgttgcgtg aaacgtcctt tgtgtatacc cgccgcgaac
     3481 cgctgggcgt agtggcaggg attggcgcat ggaactaccc gatccagatt gccctgtgga
     3541 aatccgcccc ggcgctggcg gcaggcaacg caatgatttt caaaccgagc gaagttaccc
     3601 cgcttaccgc gttaaagctg gctgaaattt acagcgaagc gggcctgccg gacggcgtat
     3661 ttaacgtgtt gccgggcgtg ggcgcggaga ccgggcaata tctgaccgag catccgggca
     3721 ttgccaaagt gtcatttacc ggcggtgtcg ccagcggcaa aaaagtgatg gctaactcgg
     3781 cggcctcttc cctgaaagaa gtgaccatgg aactgggcgg taaatcaccg ctgatcgttt
     3841 tcgatgatgc ggatctcgat ctcgccgccg atatcgccat gatggcaaac ttcttcagct
     3901 ccggtcaggt gtgtaccaat ggcacccgcg tcttcgttcc ggcgaaatgc aaagccgcat
     3961 ttgagcagaa aattctggcg cgcgttgagc gcattcgcgc gggcgacgtt ttcgatccgc
     4021 aaactaactt cggcccgctg gtcagcttcc cgcatcgcga taacgtgctg cgctatatcg
     4081 ccaaaggcaa agaggaaggc gcgcgcgtac tgtgcggcgg cgatgtactg aaaggcgatg
     4141 gcttcgataa cggcgcatgg gttgcaccga cagtgttcac cgattgcagc gacgatatga
     4201 ccatcgtgcg tgaagagatc ttcgggccag tgatgtccat tctgacctac gagtcggaag
     4261 acgaagtcat tcgccgcgct aacgataccg actacggcct ggcggcgggc atcgtgacag
     4321 cggacctgaa ccgcgcgcat cgcgtcattc atcagctgga agcgggtatt tgctggatca
     4381 acacctgggg cgaatccccg gcagagatgc ccgttggcgg ctacaaacac tccggcattg
     4441 gtcgcgagaa cggcgtgatg acgctccaga gttacaccca ggtgaagtcc atccaggttg
     4501 agatggctaa attccagtcc atattctaac caggaggttt atttgcaatt tgactacatc
     4561 attattggtg ccggctcagc cggcaacgtt ctcgctaccc gtctgactga agatccgaat
     4621 acctccgtgc tgctgcttga agcgggcggc ccggactatc gctttgactt ccgcacccag
     4681 atgcccgctg ccctggcatt cccgctacag ggtaaacgct acaactgggc ctatgaaacg
     4741 gaacctgaac cgtttatgaa taaccgccgc atggagtgcg gacgcggtaa aggtctgggt
     4801 ggatcgtcgc tgatcaacgg catgtgctac atccgtggca atgcgctgga tctcgataac
     4861 tgggcgcaag aacccggtct ggagaactgg agctacctcg actgcctgcc ctactaccgc
     4921 aaggccgaga ctcgcgatat gggtgaaaac gactatcacg gcggtgatgg cccggtgagc
     4981 gtcactacct ccaaacccgg cgtcaatccg ctgtttgaag cgatgattga agcgggcgtg
     5041 caggcgggct acccgcgcac ggacgatctc aacggttatc agcaggaagg ttttggtccg
     5101 atggatcgca ccgtcacgcc gcagggccgt cgcgccagca ccgcgcgtgg ctatctcgat
     5161 caggccaaat cgcgtcctaa cctgaccatt cgtactcacg ctatgaccga tcacatcatt
     5221 tttgacggca aacgcgcggt gggcgtcgaa tggctggaag gcgacagcac catcccaacc
     5281 cgcgcaacgg ccaacaaaga agtgctgtta tgtgcaggcg cgattgcctc accgcagatc
     5341 ctgcaacgct ccggcgtcgg caacgctgaa ctgctggcgg agtttgatat tccgctggtg
     5401 catgaattac ccggcgtcgg cgaaaatctt caggatcatc tggagatgta tctgcaatat
     5461 gagtgcaaag aaccggtttc cctctaccct gccctgcagt ggtggaacca gccgaaaatc
     5521 ggtgcggagt ggctgtttgg cggcactggc gttggtgcca gcaaccactt tgaagcaggt
     5581 ggatttattc gcagccgtga ggaatttgcg tggccgaata ttcagtacca tttcctgcca
     5641 gtagcgatta actataacgg ctcgaatgca gtgaaagagc acggtttcca gtgccacgtc
     5701 ggctcaatgc gctcgccaag ccgtgggcat gtgcggatta aatcccgcga cccgcaccag
     5761 catccggcga ttctgtttaa ctacatgtcg cacgagcagg actggcagga gttccgcgac
     5821 gcaattcgca tcacccgcga gatcatgcat caacccgcgc tggatcagta tcgtggccgc
     5881 gaaatcagcc ccggtgtcga atgccagacg gatgaacagc tcgatgagtt cgtgcgtaac
     5941 cacgccgaaa ccgccttcca tccgtgcggt acctgcaaaa tgggttacga cgagatgtcc
     6001 gtggttgacg gcgaaggccg cgtacacggg ttagaaggcc tgcgtgtggt ggatgcgtcg
     6061 attatgccgc agattatcac cgggaatttg aacgccacga caattatgat tggcgagaaa
     6121 atagcggata tgattcgtgg acaggaagcg ctgccgagga gcacggcggg atattttgtg
     6181 gcaaatggga tgccggtgag agcgaaaaaa tgagtcgtga tgtgaactaa cgcaggaacc
     6241 gatcagattg agagttaccg ttccagagag ggggaccgaa tccttatata aacactgagg
     6301 taactctcat gcttcatatc cagtattcat gatgcgggct ttttgctgat atttaacccg
     6361 aacattcctg atacagacta ttaatacaac cttatctgcg acagatttta ttctctggat
     6421 atattatgcg tgaacaaatc aaacaggata tcgatctgat tgagatttta ttttatctga
     6481 agaaaaagat tcgtgttatc ctttttatta tggctatatg tatggctatg gtgctgttgt
     6541 ttctgtatat caataaagac aatataaaag tgatttacag cctaaaaata aaccagacaa
     6601 cgccaggtat acttgttagc tgtgatagca ataataattt tgcctgtcag actacaatga
     6661 ctgaagatgt tattcagcga attactacat tttttcacac cagcccagat gtcaagaaca
     6721 gagaaataag gctggaatgg tcaggagata agagagcttt accaactgct gaagaggaaa
     6781 tatctcgcgt gcaggcctct attatcaaat ggtatgcgtc agaatatcat aatggcaggc
     6841 aagttctcga tgagatacaa acgccttcag caattaacag tgagctttat acaaaaatga
     6901 tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta actataagct
     6961 caccagaaat aaaaaataaa taccctgctg ccatttgcct ggctctggga ttttttctaa
     7021 gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa taccaacaaa
     7081 actctgggca gtaataaaca acattctgat agagagtgcc tgtataaagc tgaagtgaca
     7141 atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcac tgtaaatatc
     7201 ctggcattgg taataccagg atatttacag aagagcagca taaccatgac ggaacggggt
     7261 taagtcaggt tgtcctaaac gctattttca acctcgtatg ccttcttcag gtttatgtcc
     7321 agacttcata tctctctcaa caatcctcaa taatcagata caccgctttc accgggccat
     7381 gaactccgac gactttgata agctcaatat ccgccgttga actggggccg ctaatgatgt
     7441 taatgcagga aggcattcgt tcaccggcct gcgctttctg atgcaatttt tctgcgagtt
     7501 gcgctacacg cggcaggata gtgcttttac gcaggataaa aagagaatat tccgggagca
     7561 ggctcaatga acgcccgcgc tcggcggcgg aaaaaagaac cacgcctccc gattcggtta
     7621 aaccatattc agcatacaca acacccactt tagcctgctc tgcctgcgag atattctcgg
     7681 cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt tcgctaatcc
     7741 ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc agctctttac
     7801 acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag cgcgtcaaca
     7861 taacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta agttgggtaa
     7921 gccgctcgtt agcatagttg ttaagcggcg catcttctgc ttgcggttca agtcgcagcg
     7981 ggcgacccag tgcctgagca acgttattca aaaattcgcc tcgattatcc attctttttc
     8041 tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc ttcaggaaga
     8101 tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt gccgccattg
     8161 ataaaccagc ttgccgcatg agcaccggcc atcatcccga ctttccacaa tcctggatga
     8221 ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt gatccctttt
     8281 tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg caccggacac
     8341 acgttgtcac aagctgtgca taaagagcag gcgtagggta aatctttaaa atctttatag
     8401 ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga gccatatcca
     8461 tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca gcgaatacag
     8521 cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc gacaataacc
     8581 agatgaaact cttcaggacc atcaacgtgc ccagcttcgc gcggtcctgt cagccaggtg
     8641 ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat caatacatct
     8701 acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg cgttttaggc
     8761 agcgtggtac acattcgcgc attaccttca ttggtcacca ggcataccga accggtctct
     8821 gccaccgcga aattacagcc ggtaatacct atttcagcac tgaggaaatc ttcgcggatt
     8881 ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata gcccagacgt
     8941 tcgtgtagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac cacaacatga
     9001 gatggcggat cttgatccag ctggagaata tattcaccca gatcggtttc aatcacctga
     9061 atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat cgatttagat
     9121 ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg ggtagcgtct
     9181 tctttggttc ttgcaaaata gacgtgaccg ccgttttgcg tcactttttc tgagagctgg
     9241 tacagataag cgtcgagatt actcagaaca tgatcacgta tctgggcggc ccgatcgcgc
     9301 cactcctccc agtgccccaa ttcatcgacc attttttgcc gatttgcccc aatacgctgc
     9361 tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc
     9421 ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc tcatcaacac
     9481 ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc cactgatgtt
     9541 tagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca tcaggtgcgc
     9601 aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga acgtgccgcc
     9661 aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac gcacattttt
     9721 cagcagcgta agtggctcgt ccttcactcc cagcttacgg gccaggctac aagatgggtg
     9781 atacaccgct ctcccttgca aactggcacc tacatcgact acccctaatt tattaacaat
     9841 aaaagaggtg agatcctgca tacgcgcggc aacctttgcg gcacgtgatg cccattcagg
     9901 ttcatccgcc agatacgtcg ggtaactttt tacggcatag gtgcaagagc cagccggtga
     9961 aataatggga tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc
    10021 ttctttgata taaccgctat tgatcgcagg ctgaccgcag catccctgtt tctccgggaa
    10081 atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca ttcttgattt
    10141 cagggcgtca ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact
    10201 tcatgcccat ttatgcccga tgtttccccc ccaccggggg atcaatcatc aataaatatg
    10261 gcgttctttt ttaattggct agcaacatac cacccctcat ttgtatgaca aatgataatc
    10321 tttcatccaa acgtcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt
    10381 atggggaaat cgacgatcat ttttggcata agaaatccat ttaaaaatgg ctacatccct
    10441 tatacctatt cagagctact tattcagggg ttattgagat atacactgat ggtgtacaaa
    10501 aaaatcaccg acaataatca cgtatcaagt aatagtcggg attttattta attgaagcaa
    10561 tttagtttgc atttttattt aaaaactgag ctagagtcca tcagaaaaaa tatataaagc
    10621 ccaaaccacc gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta
    10681 tctttgccct gatttctggc ggttttattc agggtgcaag ctgtctgacc ctttcccgat
    10741 attctcccgg ggtacaacca aactcgcgga caaacgcctt gtgaaaagat gattcactgg
    10801 catagcctac tgactcagcg atcaccacaa cagggagcgt ttcccgggaa aacatctggg
    10861 ccgctatttg tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac
    10921 ggaaaagctg ggcaaaactt gcccgggaca tgtgagcgat gctggccagc gattcgacgg
    10981 tccaggcgtg tcctggcatt tccagcattt gctgtattac cgcaccaaga cgtggatgca
    11041 gaagcaaact gagaatgttt ttctcagtat taacctgtgc aatccactca cgcaccgcga
    11101 gggtaaagaa tgtagcgcag atctggctac acagtgcatc cactcccggc attgccgatc
    11161 tggattcctg ttgtaaaaac gggatcgcct ccctcagcca gttatattcc acgctgtggt
    11221 taaccggtgc taaaaacagc gtttccggca aagacgttaa aaaataacgc gccgaatgtt
    11281 gcaaccgaag agtgccgcag acaatacagg tcgattcatt atcgacatga ctcaggcgat
    11341 gagctgaatt ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctcccccg
    11401 tcggcatttc cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata accgataatt
    11461 ccccggcacc atgtggaagc tgccagtcac ttcctaacac gcaattctta tcgatcgttc
    11521 cttgtggagc gttaagcatc aacaaacggc taagggcatc catatcagac tcctgagtgg
    11581 tttttgatta caaacaccaa caatcttagt aaataactaa tctaatacca tcaaatagtt
    11641 aatcatgatt tttgttttgt gattctgatt aagaaaaaga aaatatcaga cgataagaca
    11701 aaatatcaag cgttgtgaag aaatgttatt tgctcttttg cgtctatagt catgatgtca
    11761 aatgaacgcg tttcgacagg aaatcatcat gaataaatat caggcagtga ttattggttt
    11821 tggcaaggct ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gagtggctct
    11881 catcgaacaa tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gcatcccaac
    11941 caaaacattg gttcatgacg cacagcagca cacagatttt gtccgtgcca tacagcgtaa
    12001 aaatgaagtg gttaattttt tacgtaataa gaattttcat aatcttgcgg atatgcccaa
    12061 tatcgacgtg atcgacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg
    12121 gcctgaggga aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac
    12181 cgtggttccg ccaattcctg gaattaccac cacgccagga gtatatgaca gcaccggatt
    12241 acttaatcta aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt
    12301 tgagttcgcc tctatgttcg ctaattttgg cagcaaggta accattttag aagcagcttc
    12361 gctgtttttg cctcgggaag atcgggatat tgctgataat atcgcgacga ttttacgcga
    12421 tcagggcgtc gatattatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca
    12481 agtgcaagtg catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg
    12541 tcgtcaaccg gctaccgctt cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg
    12601 cggggcaatt gtcgttgaca agcgattaca taccaccgca gacaatattt gggcgatggg
    12661 agatgttacc ggcgggctgc aatttactta catatcactg gatgattacc gcattgtacg
    12721 tgatgagtta ctgggtgaag gcaaacgtag tactgatgat cggaaaaatg tgccttattc
    12781 cgtatttatg acaccgcccc tgtccagggt tggtatgaca gaagaacaag ccagagagag
    12841 tggtgctgat attcaggtgg tgacattgcc tgtagctgca attccgcgtg ccagagtgat
    12901 gaatgatact cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatgttagg
    12961 ggcatcactg ctgtgtgttg actcccacga gatgatcaat atagtgaaaa tggtgatgga
    13021 tgccgggctg ccttatagca tattacgcga tcagatattt actcatccgt cgatgagcga
    13081 atcactcaat gatctatttt cattagtcaa ataaactcaa aatcagacgc cagaacaaat
    13141 attctggcgt ctcagagaaa agaatcttat taattctctg ttactctata acttcttaat
    13201 cacttcattg atggtatttt atatgtttaa aaaatctgtt ttatttgcaa cactattatc
    13261 tggcgttatg gcattttcca ccaatgcaga tgataaaata attctgaaac atatcagcgt
    13321 ctcgtcagta tcagcatcac cgacagttct ggaggatacc attgctgata tagccagaaa
    13381 atataatgct tcatcctgga aagtcacatc gatgcgaatt gataataatt caaccgcaac
    13441 agcagtattg tataaataag gatgttcaca atggaaaaat acctgcacct gttaagtcgg
    13501 ggagataaaa ttggcctgac attgattcgt ctgagtattg caattgtttt tatgtggatt
    13561 gggttattaa agtttgtccc ttacgaggca gacagcatta caccattcgt cgcaaacagt
    13621 ccactaatgt cgttctttta tgaacacccg gaagactata aacagtatct gactcacgaa
    13681 ggcgaataca aaccagaagc aagggcatgg caaacggcca ataataccta tggtttttcc
    13741 aacggtcttg gcgtcgtgga ggtgattatt gctctgctgg ttttggctaa tcctgtcaat
    13801 cgctggttag gtttattggg aggactgatg gcatttacca cgccgctggt aacactctca
    13861 tttttaatca ccaccccgga ggcatgggta cccgcattgg gtgacgctca tcatggtttc
    13921 ccttatttat ccggtgctgg tcgcctggta ttgaaagata ctctgatgct ggcaggtgca
    13981 gtcatgataa tggcagattc ggcgcgggaa attcttaaac aacgcagtaa tgaatccagt
    14041 tcaacgttaa aaactgaata ttgataatcc gaacactgtc tgttgttcac tttttcaggc
    14101 gtcaggcccc ctttctgatg atgaagcctg acgccatatt accggagata tgacatagtg
    14161 aagatcaata tcactccctg ttaaaaggtt tttgtcgtct taggtaaaat acatgattaa
    14221 tatcaatcat gtatttttac agcaagacaa ccacgaacaa attcaggatt aaaatgtttt
    14281 accgactccc caacataacc caaatcaagc gaactaattt gtgccatttc tttatctgtc
    14341 agtgagaaat cgatttttga tgggcatctg caatactctg aagcagacta tacggaaaat
    14401 tccactgtca gtccctccat taggcatgaa caatgagtct acgttaaaac gtaacctcaa
    14461 agtagtatgt ggattttgat atcacttatg caaaaaattc attaataatg taggactgaa
    14521 acctctctat tttcggggac aacgaagcag acgctaccag tgcttttgcc ttcgcccttg
    14581 ctatttttga tacacttagg gcccagggta taacgaaaat gtgcgatatg acaaagaatt
    14641 aacggagaat gagatgatca ggcagaagat tctacagcag ctcctggagt ggattgagtg
    14701 caatcttgag caccctattt caatcgaaga tatcgcacag aaatctggct acagcagacg
    14761 caacatccag cttctgttcc gaaatttcat gcatgtgcct ttgggagaat acattcgcaa
    14821 acgaaggctt tgtcgtgccg ccattcttgt ccggctcacc gcgaaatcta tgcttgatat
    14881 tgcactctct ttgcattttg attcacagca gtcattcagc cgcgaattta aaaagttatt
    14941 cggctgctct ccccgtgaat accgccaccg tgattattgg gatctcgcaa atatcttccc
    15001 ttctttttta atgcgtcaac agcaaaagac ggagtgtaga ttaatcaact ttcctgagac
    15061 acctattttt ggcaactcat ttaaatatga cattgaagtg tcgaataaat caccggatga
    15121 agaagtcaaa ctacgacgtc atcatttagc cagatgtatg aagaatttta agacggatat
    15181 ctatttcgtt tccacgtttg aaccgtcaac aaaatcggtc gatttgctca cggttgaaac
    15241 ttttgctggt acggtatgtg aatatgctga catgccaaaa gagtggacaa cgacccgagg
    15301 actttatgcc tctttccgtt atgaaggaaa ctgggaaaat tatcctgact gggtgcgtaa
    15361 catctatctg atagagttac ctgccagggg gttagccaga gtgaacggca gcgatattga
    15421 gcgcttttat tacaatgaag atttcgtaga aaaggatggc aatgatgttg tttgcgaaat
    15481 ttttattccc gttcgtccgg tttagttggt cactatctct tattgagttt tatccttgcc
    15541 tgatactatt taatcagtat caggcaaggt attcagtgaa taatggcgtt gaatatttca
    15601 acaccattat tctttattag atcgtaactt tcatactatt caaattatgg catctcctcc
    15661 tcgccatttt cagtctctgc agcaacggcg ttaattgtca ccttcacagg cgtcatttcg
    15721 tacccgccat acgtcagtgc gttgaatgtg aacgtataga cacctggctt gctggtacta
    15781 aatgtacgcg tgattttccc acctgagaaa tggtcagcct tcgacggcag gaattgataa
    15841 tccttctccg tcactccttc cggagcctca atgtcgaccc acatacttcc tcctacggga
    15901 ttgactcccg ccatcagggt aaccgtcatt gttgcagtag tcttcccgtc cgccaccaga
    15961 gaatctacac gttctggaga tgcggcgagc accgcgtttt tatcatccgc gacgaattta
    16021 gattcagcag ttttcgtcga tacctgaccg ttggcctgtt ccactttcgc cgtgagtgta
    16081 tatgtcgctg cgatagtggt agagccggtg aaaacagcct gtccttgctc attagttttc
    16141 gccgtccctt taggatccag aactaaattt tccgagctgg cagtcagcgt cacgtcgcta
    16201 tccttcaaca ggttgttatt ggcatcagtc accgtaactt tatacgtctg tttggttttg
    16261 ccatccgcca ctgcacggtt gccaatcacc tcaatactgg agatggtcgc cgtagtctta
    16321 tcggccacaa acgttacaga ctgcgcattc gaaggctggt catttcctgc cgacgccgtg
    16381 atctcatacg ttccggcagt cacggagacc actttcagtt ccgctttacc ctccttgtcg
    16441 gcgttcacca tgatattacc gtctgcaagc ggtttgacgc cccgaggcag attaaagacg
    16501 accacagcgc caggcagaag gttgccgtat tgatccttaa ccgttgccgt cagcgtaaag
    16561 gcatcattgt cgtttgccac ttttggcgtg ctgccatcaa cctccagggt cgcctgaccg
    16621 gtactgaaat ccgccttgaa tttcaccgta accgtcttct gagcattatt cactgaggcc
    16681 gtgatgctgt gttcccccgc aaccgttgac ataagctcaa tgtccacttt ccctgccgca
    16741 ttggttgtta ctgtattccc cgtcttgctg gtcacaccct gcggtaaagt cagcgtaact
    16801 tcttgcccct gcaacgggtt accatagctg tccacgacgg tcagggttat ctggttagca
    16861 gactgtccat tagccagttg gttattaacc ttcactgtca tatcacgaat ctcagcctta
    16921 gatgcgtcac ctgcaacgtt cagcaccagt ggctgagcaa cggcattttg gccgttcact
    16981 cgcggcatca cagacaactg acccgcggca gatcccagcg ttaaggtcgc cacgtagacc
    17041 ccattacctt tctctgtcca gtttcctgct gaaggacgct cactccccgt gctggcggca
    17101 ccgctaaaca ctggtgcatc tggcttcagc ccggtgaccg ggttcccgta cgcatccttc
    17161 acggtgaagg tgagttccgt cgtcattttg acggtcggag ccttattgtc cgcaacaagc
    17221 gtagagttcg ctgatgacat ctctccggcg atgaccgtca actgcgcggc ttcggtggct
    17281 gctggctggc cgttgaagag cggcatgaca cgaagctcgc ccgtctttcc gcctgtagtt
    17341 aacgtagcaa cataggaaca gtcacctttt tcggtccaac tggaaacggt cgccccttca
    17401 gaggcggtcc ccgtcaaact tgccgacaac gaaagaccac tgatagtgtt gccatgtgca
    17461 tcttttgcaa taagcgtcac ggttgtgctt tcgccggctt ttacgtgatc ttctgcgaca
    17521 gagactttcg actggtttga agataacgca tcagccacca cggttacttt tgccgcattt
    17581 gctgccgcat cctgtccatt tagcttcggc ataacttcta attcacccgc cgtagagccg
    17641 agagtaatct gcgcagtcca cgtcccatca ccattatttg tccagccaga tgccgtagaa
    17701 cctacagcag cggcacccgc taatgacggc gcttccggcg tgaggctggt cacagggttg
    17761 tcataggcat cttttgccgt ccagattgcc gtaactgtac cgccaaccac cggtttatca
    17821 ggattcaggg tgatggacga atgtgctgca tcaagcggac cggcaacaaa cttcagcgtt
    17881 tgttgtaatg tgcccagctg atactgctcg ctgaaagcac gcacaacaac gtttccggca
    17941 cgggtcgaag aaaccgtggc gctgtaaacg cctggtttta tttccgaaat ggcaccaacg
    18001 gttacaccat tagtgtcttg cggaacaaat cgcaagcggc tggcttctcc cgtcaccgga
    18061 ttaccctcgg agtccaccgc agtcaacgtc aacgtatagg cttgctgacc atcagcaacc
    18121 acatcacctg acggctcgtt agcgctcagg gtggagtttg ccacatccat catcgtggcc
    18181 cgcagttctg cagtgacggt tttgctcatg ccatcaacgc taacagtaat cgttgcctca
    18241 cctgactgcg ttccggtagt aaagacagac tgatacaccc ctgcttcagt ttcggtgaac
    18301 tcacccagtg ttggctttgc ctgtgattta gtggccttca gggaacgagt cacaatattt
    18361 ccagccggtt tgaaggctag ttcagtcttg atctgatctt tcatgcccgt gactggctgc
    18421 ccctcggcgt cgcgcagaga cagcaccagc ggtctttgct cattaccgtt agcaagcatt
    18481 tgaatacggc tctgaccgtc aagcgttaac gccgtgcgat cggcgctcat acctgctccg
    18541 gtaatgacca cctctgtctg cacgcgtttt gaggcattgc ctttgttatc gtaggcaacc
    18601 gcagaaatcg cataataatt gtctttgcct ggacgataag ccgggagcgt tacttgccac
    18661 tgactaccct gaccggtaat tttgccaccc tcagccagta atgacggcgc ttcccactgc
    18721 acatttttca gtccgtgagt tgctttgctg accacaagcc ccagggaaag tgtctgacca
    18781 cccttacctt caatacgttc aggcagagca atacggatca cttcagattt gcggtactca
    18841 agaacgatgt tgttattacg ctcaaccagg tcatagcggc tgcctgccag tacccgacgc
    18901 tcgcgaatgc tgtctgtatc gagttgtttc gccagaggtt cgccaatccg ataattaact
    18961 tccaggccaa agcgagtgtc attctcacca ctcttgccct gcttatgccc ggcgctcagt
    19021 gtcagaagag gcactggcgt ataggtcacc tcggcagaaa tagcatgcgg gtctttctgg
    19081 cgcttatctt taccaaacag cccgacttca tcgccataat actgttcata catcaggctt
    19141 gcgccaagct gcggccaggc gggtaaatag ccctcagcac gaatatccca gccattcgcc
    19201 gggcgttcct gataatcctc aatatccggc gattttttcc agccagaagc ccgaatataa
    19261 ccattggcgc tcagtttcag ataatcgcgc cagtattccg caccaacacc aatgcgggta
    19321 tgactacggg ataaatcatg atcgataaaa gtattcaccc ccgccatcca gtcatttcct
    19381 gaaaaatgac gccagccaaa accaatattt gactgagtac gatcgtctgt acgatgtatt
    19441 gccccctgag tgaacaacat atttgtcggc gtatcataaa tcggataaag catttccagc
    19501 gaagaatcct tcagcgagaa atctttatcg acattcagtt tgacgcgcgc agtaccatat
    19561 ttcccgagcc actcctgtat ttcctggtta gctttagctg tggccattcc ggtaataaag
    19621 ttacgtgtcg catcgctatc tggctgactg cttaaaaatg tcccggcatt tgcggcaaac
    19681 gacgcgacat ttttctccac gttattatca gcagttaccg tagtatttcc catgctcaac
    19741 cgtggctgaa ccgcatgctg cgcacgtgcc gccattactg gggtaaaggt gacagcgagt
    19801 ggaaaaagaa cctgaacaga gatatttgcc cacgccacgc agcgggccag aactgaataa
    19861 cgaaatcgtg gttgtttatg acctgtttta taatgtgaca ttgagataat ttcctgatga
    19921 acgaattaac gaacataggc agacacaaat aatggcgtct gcttgtcaga aaaaccgggg
    19981 atttcccgat ttccttttcc gcagaaaagg aaataccttt atgtaagaaa gtgcaccgcc
    20041 acctaatata tccagtgcct atagccactc atcaggtggt tgggcgcggg attatatttt
    20101 tatttatggg ggaaaattta gctttttgtg caagtcatta aatagatacc taagttatat
    20161 ttcttactct gcggacagtg aataattcac tattcagagt gaataacaat gacctgcctt
    20221 taattaaaac tattggaaat acgtcgaaat aggtatatat gccaggataa accaatatat
    20281 tttttatgga tattcatttt ccttcatcgg actgtaaatt gcggagatat cgaacttgca
    20341 ctacccatcg caacactcaa acagatttat gaatttcgtt cataacccct gacaaattct
    20401 gccgtctaat cgagcttttc cctttacgtt atgcttctca tgaacgataa cacaacttgt
    20461 tcatgaatta accattccgg ataaactatg ggatcaaatt caggcatcga aaggaactgg
    20521 ctggtttccg ttaatggatt aaatatctat tattctcccg agagtaaaac tatcaaatca
    20581 atgagtaacc gatacagcct cacaaattct ttcagataat taacccgtca ctgcggcacg
    20641 ttctctcata aggcgtgcct gcagtgtaat ccagtatgta tccggcatag ttgacggttt
    20701 aatagaactc aattcctctc ccaggtcacc aacaacgata tcttgtataa caccccagga
    20761 gatacgacat tatgatgaga tgcgatatgc tcctcagcac caatcaacgc ccggactggg
    20821 ccagtcttga gatcagactc attcccggaa ctgacttcag gcgacatata tcaatgacgt
    20881 tggtgcaata aaagatagtt gatgtaatac atctattcaa atttgtgata catgtcaaaa
    20941 aattggttga ccaaactcgt tatttatata agggcactta cgaagtgcac tcttttttaa
    21001 agcgaggaag taccaatgaa agagaataaa gtacagcaaa tcagtcataa actgattaat
    21061 atcgttgttt ttgtcgcaat tgtagaatac gcctatttat ttctccattt ctattaataa
    21121 cggaaataaa ctgttcactt cagtgatatt taaaatatgc atcctctccc ttttttgtaa
    21181 gtaattatta tatccgtggg agaggaatac acgctgtcag gtaatcaatc atactgcgat
    21241 aaattatcgg ccagtaaagt ggagataacc tccattctcg aaaaatccat actctcagca
    21301 aaaccatcat caatcactca tccaggcgtt tatgggagcg tcaccaatgg ctgctaacaa
    21361 tgccagagtt ccccgttgcg aaaattccac atccacaaag agtcacaggg attgagtgtt
    21421 gaaatgatcc ggatgagcat gtatctttat ggttatgtta taacataaca ggtaaaaatg
    21481 atgaagccca atatccatcc tgagtatcgt actgtggtgt tccacgacac cagtgttgat
    21541 gagtacttta aaatcggctc gactatcaaa acagaccgtg agattgagct ggatggcgta
    21601 acgtatccat acgtgacaat tgatgtctct tctaaatcgc acccgttcta tacagggaag
    21661 ctgagaacag tggcatcaga aggaaatgtt gcacgattca cccaacgttt tggtcgtttt
    21721 gttagcacga aaaagggggc gtgatgaaag ttcttaactc tctgcgtacc gcaaaagaac
    21781 gccatccaga ctgtcagatt gtgaagcgaa aaggacggct atatgtgatt tgtaaatcta
    21841 atccacgttt taaggccgtt cagggtcgta agaaaaaacg ttgattcaaa attcgacgga
    21901 ttaacgatat ttgtctgatt aataatcaga tcggattaat gttggtgtgt ttataacacc
    21961 aacattaatt ttcctgggga tatattcttc ctgttcattt gaggccaact gcctgacgtt
    22021 tctctccgaa tattccatta tcttaatgtt gacttgttga ccagcttcgc ccctgtatgc
    22081 tggcatcaac cctcttttag actgaacacg ccactcagtc tcctcccttt gcggcgcagc
    22141 ctgcattttc actcaaactg ttaagatgat aaatgtggta aatctgttgg tactaacata
    22201 aaaacgttta cgccacagga acagtctgat ccaccggtaa ccccgtcgcc gacgttcgag
    22261 tgccagttag agtaacgcgc acagataact gaatgcagtg ccctgacaaa aaggccatcg
    22321 ttcctgtgac agctggcagc cttcgtttaa cttcacttaa tctggctctt gggggcttac
    22381 cgaacagatg acgtacatac gcccgttcaa ttttccatta cttattggaa tgaacacctg
    22441 taaccatttt gtgcggcatg ttaatccatt aaaatatctt actgattggc aaatcatctt
    22501 caatgacagc tcatcatagt tttatattct atcccttacc cttaaaactt gtttttttac
    22561 tagtccatca cacagcgcat taagactatt cctaacactt cagggcaaag ttcctgacca
    22621 atataaaatg caagtaagaa ttgaacgtta tattgccaat aaccttatga aaccaaatgt
    22681 ctttttcttc ttatcaaaaa agcaatattt tcagtttttc taaatattga cttaaccatt
    22741 gaattccttt tccgttcaca tattgacact catcgggaaa aaaaacataa atttaagccc
    22801 aatcgaaaat aattaaactt aatctcgttt aacctttatt gatatgtact acgtatctta
    22861 tttacttccg gtttactaag gaaactgaat gcacctgtaa aaattacagg tttggaaagt
    22921 agtgacatgg caaagtgatt acagtaggga ctatgaggtt aaaaaccata tggaatgtca
    22981 aaaccgttct gataaataca tctggtctcc ccatgacgcc tacttctata aaggactatc
    23041 tgaactgatt gtggatatcg acagattaat ttatctatcg ttggagaaaa ttagaaaaga
    23101 tttcgtgttt atcaatctca gtacggattc tttatctgaa tttataaacc gtgataatga
    23161 atggttatcc gcggtaaagg ggaaacaggt cgtattgatt gcggccagaa agtcagaggc
    23221 cttagcaaat tattggtatt acaatagcaa tattaggggc gtggtatacg ctggactgag
    23281 tcgtgatatt agaaaagaac tggtctatgt gattaatggc aggttcctga gaaaagatat
    23341 taagaaagat aaaatcacgg accgggaaat ggaaattatc cgcatgacgg cccagggaat
    23401 gcaacctaaa tcgattgcca gaattgaaaa ttgtagtgtg aagacagtgt atacccatcg
    23461 gcgtaatgct gaggccaagc tgtactcaaa aatatataag ttggttcagt aaactccagg
    23521 caagttagtt ttaaaaaatg actcactggg acatcacgtc ctcaattcaa ctcgggaaga
    23581 aatgcaatga aaaaaaaggt tctggcaata gctctggtaa cggtgtttac cggcatgggt
    23641 gtggcgcagg ctgctgacgt aacagctcag gctgtagcga cctggtcggc aacagccaaa
    23701 aaagacacca ccagtaagct ggttgtgacg ccactcggta gcctggcgtt ccagtatgcc
    23761 gaaggcatta aaggttttaa ctcacagaaa ggtctatttg acgtggctat cgagggtgac
    23821 tcaacggcta ccgcctttaa actgacctca cgtcttatca ccaacacatt aacccagttg
    23881 gatacctcag gttccacact gaatgtgggc gtggattata acggcgcggc agtcgaaaaa
    23941 actggcgata ccgtgatgat cgataccgcc aacggcgtac tgggcggcaa ccttagcccg
    24001 ctggctaacg gttacaatgc cagcaatcgt accaccgcac aggatggttt caccttctcc
    24061 atcatcagcg gtaccaccaa tggtaccacc gcagtaacag attacagcac tctaccggaa
    24121 ggcatctgga gcggcgacgt tagcgtacag ttcgacgcga cctggaccag ttaatctctc
    24181 tgatgtacca gcaggggtag cccccctgct ttattcctgg acggaattgt tatgaaaaag
    24241 caccttctgc ctctcgctct gctgttttcc ggaatatctc cggcccaggc gctggatgtc
    24301 ggcgatatat catcgtttat gaacagtgac agcagcacgc tgagcaaaac gatcaaaaac
    24361 agtaccgaca gtggtcgcct tatcaatatc cgtctcgaac ggctctcttc accgcttgac
    24421 gacgggcagg ttatctcaat ggacaagccg gatgagttgc tactcactcc cgccagcttg
    24481 ctgctacccg cccaagccag cgaagtgatc cgcttcttct ataagggacc cgcagatgaa
    24541 aaagagcgct actaccgcat tgtctggttt gatcaggccc tcagtgatgc gcagcgcgat
    24601 aatgccaacc gcagcgctgt ggccactgct tccgcccgca tcggcaccat tctggtcgtc
    24661 gcccctcgtc aggcgaacta ccactttcag tacgccaacg gctccctgac aaatacagga
    24721 aatgcgacgc tgcggatcct cgcctacgga ccttgcctga aagccgccaa cggtaaggag
    24781 tgtaaagaga attactacct gatgccgggc aagtcgcgtc gttttacccg cgtggacact
    24841 gcggataaca aaggacgggt tgcactttgg cagggtgata agttcattcc cgtgaaatag
    24901 atagctgtgc agatggataa cgacaatgcc tttacgacgg ttctccccag gactgaaagc
    24961 ccagtttgcc ttcggcatgg tctttttgtt cgttcagccc gatgccagcg ctgctgacat
    25021 aagtgcgcag caaataggtg gggtgattat tccgcaggcc ttcagtcagg cgcttcagga
    25081 cggcatgagc gtcccgctct atattcatct cgccggtagc cagggtcgcc aggacgatca
    25141 gcgaatcggc agcgctttta tctggctgga cgatggacag ctacgcatcc ggaaaataca
    25201 gctggaagag agtgaagata acgccagtgt cagcgaacaa actcgacagc agctgatggc
    25261 tctggccaac gccccgttca atgaggccct taccatcccc ctgactgaca acgcgcagct
    25321 ggatctcagc ttgcgccaac tgctgctgca gctagtggtc aagcgcgaag cgctgggcac
    25381 cgtactacgc tcacgtagcg aagacatcgg gcagtccagt gttaacaccc tcagcagtaa
    25441 tctgagctat aacttgggcg tctataacaa ccagttgcgt aacggcggga gcaacacatc
    25501 cagctatctg tcgctgaata acgttactgc actgcgcgaa catcatgtgg tgctcgacgg
    25561 ctcgctgtac gggatcggta gcggtcaaca ggacagtgaa ttatataaag cgatgtatga
    25621 acgcgatttt gccggtcacc gatttgccgg tggaatgctc gacacctgga acttgcagtc
    25681 cttagggccg atgaccgcca tttcagcagg gaagatttac ggcctttcct ggggaaacca
    25741 ggccagctcc accatcttcg acagcagcca gtcagccacg ccagtgatcg cctttttacc
    25801 ggcggcgggc gaagtacatc tcacccgtga tgggcggcta ctaagcgttc agaacttcac
    25861 tatgggcaat catgaagtgg atacccgggg tctaccgtac gggatttacg atgtggaagt
    25921 tgaggtgatc gttaacggtc gcgtgatcag caaacgcacc cagcgggtca ataagctgtt
    25981 tagccggggg cgcggcgtcg gtgcaccact ggcgtggcag gtatggggcg gtagctttca
    26041 tatggatcgc tggtcggaaa acgggaaaaa gacgcgacca gctaaagaga gttggctagc
    26101 aggtgcctcg acctccggct cattgagtac gcttagctgg gcggcaacgg gatatggata
    26161 cgataatcag gcggtgggtg aaacccgtct gacgctgccg cttggggggg cgatcaacgt
    26221 taacctgcaa aatatgctgg ccagtgacag ctcatggagc agcatcggca gcatcagcgc
    26281 cactctaccg ggaggcttta gttcgctgtg ggttaatcag gaaaaaaccc gcattggcaa
    26341 tcaattgcga cgtagcgatg ccgacaaccg tgcaatcggc ggcacactca acctgaactc
    26401 actgtggtcg aagctgggca cattcagcat cagctacaat gatgaccgcc gttacaacag
    26461 ccattattac acggcagatt actatcaaaa tgtctacagc ggtacctttg gttcgcttgg
    26521 cctgcgggcc ggtattcagc gctataacaa cggcgacagc aacgccaata caggaaaata
    26581 tatcgctctc gatctctcgc taccactggg caactggttt agcgcaggga tgactcatca
    26641 aaacggctac accatggcaa acctgtcagc acgcaaacag tttgatgaag gaaccattcg
    26701 cactgttggt gccaatctgt cacgtgccat ctccggcgat accggtgatg acaaaaccct
    26761 cagcggcggg gcgtatgcac aattcgacgc tcgttacgcc agcggaacgc tgaacgtcaa
    26821 tagcgcggca gacggctacg tcaataccaa cttgaccgcc aatggcagcg tcggctggca
    26881 gggtaaaaac attgctgcca gcgggcggac tgatggcaac gctggggtga tattcaacac
    26941 cgggctggag gacgacggtc agatcagcgc caaaatcaac gggcggattt tcccgcttaa
    27001 cggcaagcgt aactatctcc cgctctctcc ctatggaaga tatgaggtgg agttacagaa
    27061 cagcaaaaac tcactcgaca gttacgatat cgtcagcggc cgcaaaagtc gtctgactct
    27121 ctatccaggc aatgtcgctg tcattgagcc agaggtgaag cagatggtta ccgtctccgg
    27181 tcgtatccgt gcggaagacg gcacactgct ggctaacgca cggattaaca accatatcgg
    27241 ccgaacccga accgatgaaa acggcgagtt tgtcatggac gtggataaga aataccccac
    27301 tatcgatttt cgctacagtg gcaataaaac ctgcgaagtg gctctggaac tcaaccaggc
    27361 gcgcggtgcc gtctgggtcg gtgatgtggt ctgcagcggc ctctcatcgt gggcggcggt
    27421 gacgcagaca ggagaagaga atgagagtta acctactaat aacgatgata attttcgcgc
    27481 taatctggcc agtaactgcg ctcagagcgg cagtgagcaa aacaacctgg gcggatgcac
    27541 cggcacgcga gtttgtgttt gtcgaaaaca actcagacga caactttttc gtcactcctg
    27601 gcggggcgct ggatccgcgc ctgaccggtg ccaaccgctg gaccggttta aaatacactg
    27661 gttcaggaac catctatcag caaagcctcg gctacattga taacggttac aacaccggcc
    27721 tttataccaa ctggaagttt gatatgtggc tggaaaattc accagtttca tctcctttaa
    27781 ctggcttgcg ctgcatcaac tggtacgctg ggtgtaatat gaccaccagt cttatcctgc
    27841 cgcaaaccac cgacgccagt ggattttatg gcgcgacggt gaccagcggc ggcgcgaagt
    27901 ggatgcacgg catgttgtca gacgcgtttt accagtatat gcaacaaatg cccgtcggca
    27961 gcagctttac aatgaccatc aatgcctgcc agacctctgt gaactatgac gccagcagcg
    28021 gcgcacgctg taaggatcag gcctccggca actggtatgt tcgcaacgtc acccatacga
    28081 aagcagcaaa tctacggttg ataaataccc actcgctggc ggaagtattt atcaacagcg
    28141 acggagtacc gactctgggc gaagggaacg ccgactgccg gacgcaaacc atcggcagcc
    28201 gttcaggatt aagttgtaag atggttaact ataccctgca aacaaacgga ctcagcaaca
    28261 cctcaatcca tatattcccg gcgatcgcca actcgtcgtt agcctcggcc gtcggggcgt
    28321 acgatatgca gttcagtctg aatggcagtt catggaaacc ggtgagcaat accgcctatt
    28381 actacacctt caacgagatg aagagcgcag actcgatcta tgttttcttc tcgagcaact
    28441 tctttaagca gatggtgaac ctcgggatca gcgatatcaa caccaaagat ctattcaact
    28501 ttcgctttca gaacaccaca tcaccggagt ctggctggta tgaattttct acctccaaca
    28561 cgctgattat caaaccccgt gatttcagca tcagtattat ctccgatgaa tatactcaga
    28621 caccgtcgcg ggagggatat gttggcagcg gcgagtcggc actcgatttc ggctatatcg
    28681 taaccaccag cggtaaaaca gctgccgacg aagtgctgat caaggtgacc ggacccgcgc
    28741 aggtgattgg cgggcgctcc tattgtgtct tcagctccga tgacggtaag gcgaaagtac
    28801 cgttcccggc gacactttcc tttattaccc gcaacggagc tacaaaaacc tacgatgccg
    28861 ggtgcgatga tagctggcgg gatatgaccg atgcgctgtg gttgaccaca ccgtggactg
    28921 atatctctgg cgaagtgggg cagatggata agaccacagt caaattttcg attccaatgg
    28981 ataacgccat ttcgctgcgt acggtagatg ataacggctg gtttggcgaa gtcagcgctt
    29041 caggagagat tcatgttcag gcgacgtggc gtaacattaa ctaaggccct gctgacagcg
    29101 gtctgtatgc tggcggcacc tttgacacag gcgatttcgg tcggcaatct gacattttcg
    29161 ctgccgtccg agactgactt tgtcagcaaa cgtgtagtga ataacaacaa aagcgcacgg
    29221 atataccgta ttgccatcag tgctattgat agcccgggca gcagtgaatt gcgcacccga
    29281 ccggtggatg gtgaactgct tttcgccccc cgccagctgg cgttgcaggc tggtgagagc
    29341 gagtatttta aattttacta tcatggtcca cgggataacc gcgagcgcta ctaccgggtc
    29401 tcatttcgcg aggtccccac tcgtaaccat acaagacgca gcccaaccgg cggcgtggtc
    29461 agcacggagc cggtggtggt gatggatacc attctggtag tacgaccacg tcaggttcag
    29521 tttaaatggt ccttcgacaa ggtgacagga acggtaagta acactggcaa cacatggttt
    29581 aagctactga ttaagccagg atgtgattcg accgaagagg aaggcgatgc ctggtatcta
    29641 cgtccgggag acgtggttca tcagcctgag ttacgtcagc cggggaatca ctatctggtc
    29701 tataacgaca aattcattaa gattagcgat tcctgtccgg ctaagccccc ttcggcggac
    29761 taagacttat ccatcggtcg agggaagaat tccacctcag agccccaaaa atcgtttaaa
    29821 tgatgtggaa gcgatcgtca atatcacctg taacgatggg ctttctcata tgcacaccat
    29881 agtgttcagg agacacggaa aggtatcagg cagcctgcta acgagtggtt taatcacgaa
    29941 ttagtacgta aaatcggtaa cggctggaaa tcattcaata ctcacactat cgaaagttcc
    30001 ccagccaacc gcggtacgtt cttacatacg atgtaccgct gttctcttta cgatttttag
    30061 ctgtactggt gaattatgag caatctgaat ccatgcatga cgtgtggtgc ctgttgtgca
    30121 tttttccgcg tctcttttta ctgggccgaa gccgacgatg ctggcggtac tattcccgcc
    30181 aggctcactg aacaaatatc cccttttcac cgatgcatga gcggtaccaa tcagaaaaac
    30241 ccccgatgta ttgcccttgc aggaaccccg ggcaaaaatg cctgctgcac gatatataaa
    30301 aatcgatcgt ccacatgcag agaattcgcc atgtctggtg agaacggagt cgtcaatgag
    30361 gcttgcaatc gtgcaagggc taaatacggg ctgacaccgt tataaacata caataattaa
    30421 ttgcactgcc ccgccagcga taatagcggg gcttcgtttt tcaggggtaa caaaacccga
    30481 tacttcttct atttgccagc aacaatgcct ctcttctgta gcgttcttcg gatctacatc
    30541 atcctgaagt agcgcgatat actcactgac tatctgcatc agaatataaa aagcaatgtt
    30601 tttaacctat aaaaatggcg ctgtatttgc gccattttta tcattcaatg cattatctgt
    30661 ttgagcctaa agggatctca gggtctggct catgagtaat tctgtttcgt aaatctctgc
    30721 gaataatttc aatagaccag aaccagacta aatgtccaaa aatttcagaa acattctcat
    30781 accacgggag atcaaacaga ggtggcgtca gtcccatgag agggaatgaa atcatatgaa
    30841 taaaaagttg ggctaaagca cctgccagta agccctgcca gagtttaatt tttggaaata
    30901 cttcagcgac cacacaataa ccgacagcaa acactatcga aaagataatg tgcgtaacac
    30961 caacccagtt aaagacatgc ccggcaaagg tataaacagc cgcattggga tctgtcagcc
    31021 ccaaccagtc tcgaagaaaa atatacggtg gattgagaaa attacgcgag caatcaattt
    31081 ggcctgcagc cctgattaat gattccgggc cacacgctgc attaaacata tccaccgggc
    31141 tacgtggcgg caatggaact tcagcccccc acttcacgaa tgcggaaaca acgccagcaa
    31201 tcagcccaat gaatgcagca agaccataac gtctgcggtt cggtggagtt tgttcaaata
    31261 tattcatatc taccctgctt gtaccattat gttatacacc tcttcaggag tattcataaa
    31321 acaaggcaaa tgtaaagaac tgtattgttt tgtataacaa gatagtttcc taatcgccaa
    31381 tgaatataag ctccatcatt tctccctatt tttatattag aagtgacaga gatttgcagg
    31441 gtgatgtaga gctgaaaatc acaggtttcc ttattggttt ttgcatcgta caactaaagc
    31501 aataaaccag ctcccatcat catcaacatc ccggcgaaca tgaatttatt agccgggctc
    31561 atagctgcat tcgcgtttta agaataatcc tcctgctgtc gccgactatg cttaacgttt
    31621 aaaaaagcat cagcactctc gcaacgcact cttattttcc cctttagaat accggaggcc
    31681 tggtatgagc aaccaaggcg aataccccga agataatcgg gttgggaagc acgagccgca
    31741 cgatttaagt ttgacccgtc gcgatctgat taaagtgagc gccgcaacag cggcgaccgc
    31801 cgtggtttat cctcattcta cgctggcggc aagcgttccg gcagctacac ccgcgccaga
    31861 gataatgccc ctgacactga aggtgaacgg caaaaccgag cagcttgagg tggatacccg
    31921 aaccacgcta ctggacactt tgcgtgaaaa tctgcatttg atcggtacca agaaaggttg
    31981 cgatcacgga cagtgcggag cctgtaccgt gctggtcaat ggtcgcaggc ttaatgcctg
    32041 cctgacgctt gcagtcatgc atcagggggc cgagatcacc accattgaag gcctgggttc
    32101 gccagataat cttcacccca tgcaggcggc ctttatcaag catgatggct tccagtgcgg
    32161 ctactgcacc tccgggcaaa tttgctcatc agtagcggtg ctaaaagaga ttcaggacgg
    32221 cattcccagt cacgtcacgg tcgatttggt ttccgctcca gaaacaactg ccgatgagat
    32281 ccgtgaacgt atgagcggca acatctgtcg ctgtggtgca tacgctaaca tccttgccgc
    32341 cattgaagat gctgcggggg agataaaatc atgaaggcgt ttacctatga acgagtgaat
    32401 accccagccg aggcggcact tagcgctcag cgcgtacccg gcgcaaaatt tatcgcgggc
    32461 gggaccaatc tgctggacct gatgaagctg gaaattgaaa cgcccaccca ccttatcgat
    32521 gtgaacggcc tcgggctcga taagattgaa gtgaccgacg cgggtgggct gcgcatcggc
    32581 gcactggtac ggaacaccga cctggcggct cacgagcgcg tgcgtcgtga ttacgcggta
    32641 ctctcccgcg ccctgctcgc tggcgcgtct ggtcagttac gtaatcaggc aaccaccgca
    32701 ggtaatctgc tccagcgcac gcgctgcccc tatttttacg acaccaatca gccctgcaat
    32761 aagcgcctgc ccgggagcgg ctgcgcggcg cttgaaggct ttagccgtca gcacgcggtg
    32821 gtaggcgtaa gcgaagcctg cattgccacc catccgagcg atatggcggt cgcaatgcgg
    32881 ttgctggatg cggtggtgga aaccatcacg ccggagggaa agactcgcag tatcacactg
    32941 gctgattttt atcaccctcc gggaaaaacg ccgcacattg aaaccgccct gcttcccggt
    33001 gagcttatcg ttgcggtgac gttacctcca ccgctcggcg gaaaacatat ctaccgtaag
    33061 gtgcgcgatc gcgcctccta cgcctttgcc ctggtatcgg tcgcggcgat tattcagcct
    33121 gacggcagcg ggcgcgtcgc gctgggcgga gtagcacata agccctggcg cattgaggct
    33181 gcggatgctc agctatccca gggggcgcag gccgtatatg acacgctgtt cgccagcgcc
    33241 catcccaccg ctgaaaacac ctttaaactc ctgttggcga agcgaacgct tgcctccgta
    33301 ctggctgaag cgagggcaca ggcatgaaat ttgataaacc cgcaggggaa aacccgatcg
    33361 atcagctgaa ggttgtcggt cgtccccatg accgcatcga cggaccgctg aaaactaccg
    33421 gcacggcacg ctacgcctac gaatggcatg aagaagcccc caacgccgcc tatggctata
    33481 tcgtcggttc cgccattgcc aaaggacgcc tcaccgccct tgatacggac gccgcgcaaa
    33541 aagcgccggg cgtactggct gtcattaccg ccagtaacgc cggggcactc ggcaaaggcg
    33601 acaaaaacac cgccaggctg ttaggcggcc ccactattga gcactatcat caggccattg
    33661 cgctggtagt ggccgagacc ttcgaacagg cgcgagcggc ggcctcgctg gtgcaggcac
    33721 actatcgccg taataaagga gcttactccc tggcggacga aaaacaggcc gtcaatcagc
    33781 cgccggaaga cacgcccgac aaaaacgtcg gtgactttga cggggctttc acctccgctg
    33841 cggtgaagat tgatgctacc tacacgaccc cggaccagag ccatatggcg atggagccgc
    33901 atgcctcgat ggccgtctgg gatggaaata agcttaccct ctggacctca aatcagatga
    33961 ttgactggtg ccgcaccgat ctggcaaaaa cgctgaaagt tcccgtggag aatgtgcgta
    34021 ttatctcccc gtatatcggc ggagggtttg gcggcaagct gttcctgaga agcgatgcgc
    34081 tgctggcggc cctcgccgcc cgagcggtga aacgtccggt taaagtgatg ctcccccgcc
    34141 cctctattcc caataacacc acgcaccgcc ccgccaccct tcagcacttg cgtatcggtg
    34201 ccgaccagag cgggaaaatc accgctatct cacatgaaag ctggtctgga aacctgcccg
    34261 gcggcacgcc ggaaacggcg gtacagcaaa gcgaattact ctacgccggg gcgaatcgtc
    34321 ataccggcct gcggctcgcc acgcttgatt tgccggaagg gaacgccatg cgtgcgcccg
    34381 gcgaagcccc cggtctgatg gcgctcgaaa tcgcgatcga cgaactggcg gaaaaagcgg
    34441 gcatcgatcc cgtcgagttt cgcatcctga atgacactca ggttgacccc gccgacccga
    34501 cgcgctgctt ctctcgccgt cagcttatcg agtgcttgcg caccggagcg gataaatttg
    34561 gctggaagca gcgcaacgcc acacccggac aggtgcgcga cggggagtgg ctagtcggcc
    34621 acggtgttgc ggcgggcttt cgcaataatc tgctggaaaa atcgggtgct cgggttcacc
    34681 tcgaacaaaa cggcaccgtt accgtagaaa cggacatgac cgacattggc accggcagct
    34741 acaccattct ggcccagacg gcagcggaaa tgcttggcgt accgctggag caggttgcgg
    34801 ttcacctcgg cgattccagt ttcccggttt ctgcgggttc tggtggacaa tggggcgcga
    34861 atacctccac ctccggcgtt tacgccgcct gtatgaagct tcgcgaaatg attgcctcgg
    34921 cagtcgggtt tgatcctgag cagtcgcagt ttgccgacgg caagattacc aacggtaccc
    34981 gaagcgccac gctacatgaa gccaccgcag gcggcagact gacagcggaa gagagcattg
    35041 aattcggaac actgagcaaa gagtaccagc agtcgacctt tgccgggcat tttgtggagg
    35101 tcggcgtgca tagcgcgacg ggagaagttc gggtccggcg tatgctcgct gtgtgtgctg
    35161 caggacgcat cctgaatccg aaaactgcgc gcagccaggt cattggcgca atgactatgg
    35221 gcatgggcgc ggcactgatg gaggagctgg cggtggatga ccgtttgggc tacttcgtta
    35281 atcacgatat ggcggggtat gaggtgccgg ttcatgcgga tatcccaaaa caggaggtga
    35341 ttttcctgga tgataccgac cccatatcct ccccgatgaa ggccaaaggt gtcggtgagc
    35401 tgggcctgtg cggcgtgagc gcggctatcg ccaacgcggt gtataacgcc accggtattc
    35461 gggtacggga ttatcccatc actctggata agctgctcga taagctgccg gatgtggttt
    35521 aaggaggaac aatgtcatac ccgctttttg acaaagacga acactggcat aagccagagc
    35581 aggcgtttct caccgatgac caccggacca ttctgcgctt cgccgtagag gcgctaatgt
    35641 ccggtaaagg agcggtgctg gtgacgctgg tggagatacg cggcggcgcg gcgcgcccgc
    35701 tcggggcgca gatggtggtg cgcgaagatg gtcgttactg cggttttgtc tctggcggct
    35761 gcgtggaggc cgctgccgct tttgaagcgc tggagatgat gggctcaggc cgcgatcgcg
    35821 aaattcgcta tggcgaaggt tcgccgtggt ttgacatcgt tctgccctgc ggcggtggga
    35881 tcacgctgac gctccataaa ctacgctcgg cacagcctct gctcgccgtg ctgaaccgcc
    35941 tggaacagag aaaaccggtg gggctgcgct acgatccgca agcacaatcg ctggtgtgcc
    36001 tgcccacgca aacccggacg ggctggaatc tcaatggctt tgaggtgggg ttcaggccat
    36061 gcgtcaggct gatgatttac ggacgttctc ttgaggcgca ggcaaccgcg agtcttgcag
    36121 cagccacagg ctatgacagc catatcttcg atctttttcc ggcctcagcc agcgctcaga
    36181 tcgataccga tacggcggtc attttgctgt gccatgatct caaccgggag ctgccagtgt
    36241 tgcaggccgc gcgagaagca aaaccctttt atctcggcgc attgggcagc tatcgaaccc
    36301 acacgttacg tctgcaaaag ctccacgagc tgggatggtc cagggaggaa acaacgcaaa
    36361 tccgggcacc cgtcgggata ttccccaaag cccgggatgc gcatactctg gcactctccg
    36421 tgctggcaga agtcgcctct gtacgtctcc atcaggagga ggattcatgc ctgcccccgt
    36481 cgtcctgatc cttgcggccg ggcgtggaga gcgctttctc gcctccgggg gaaataccca
    36541 taagtgtatc ggctggcgtc agtccccgga ggttgcgcct tatcgctggc catttgaaga
    36601 aaacgggaga actttcgacc ttgcgattga accgcagatt acgactaatg atctgcgtct
    36661 gatgttgagg ctggctcttg ccggcggagg aataaaaatt gccactcagg aaactttcag
    36721 gccatatatt gaaagcggta agcttgtatc gctgcttgat gactttcttc cacaatttcc
    36781 gggcttctat ctgtatttcc cacagcgtcg caatattgca ccaaagctcc gcgccctgat
    36841 tgactacgtc aaagaatggc ggcagcaatt ggcttaaatg tctgcacctg cactgcctga
    36901 tgtcagaaca gtattttgat gaattgccaa ggttacaatg gcacaaatac ggcacaggag
    36961 gaaacgtggt gtatttaaat atggggtaac tctttgattt taatggtgcc gataatagga
    37021 gtcgaaccta cgaccttcgc attacgaatt agcgagacca gtgattcaag atgttcacag
    37081 ccgtttcagc atacaataaa aaacatataa aaatcaacaa aatatacata aatgcagtat
    37141 caagatgttt cgccttgttt ctgatgatat catgttaaat gtgtaccccg ctgtgtaccc
    37201 aaaaggctga agatatgact tttactatta cacaagacag acaattggct tcattgaccg
    37261 ttactaaaga agaaaaagaa atcttatttg ctgtaaaatc taaagctggg ggaggactct
    37321 ttatacgtgt tcgttatggt agcgacaata aaagctggat ttatcgttat cgaatagctc
    37381 aaaagccagc taaattaaca ctaggtagtt accctgccat gggattagct caagctcgta
    37441 aagctcatgc ggatgcaatt gaattagtaa gaaaaggtat agatcctcgt catattagaa
    37501 aaaatgaaaa acaaaaaaat gaacaaatga ttatttactc tgaattatgg aaagattgga
    37561 tagaattcag aaagacaagt aaaccaatta gcaccagaac ccttgcagat tatgaaggga
    37621 cttatcaccg ccaccttgaa aaagcattag gaaaccttcg cattgacgaa cttaccaaaa
    37681 gcgttatttt tgaacatctc agtaatgtaa gaaaaaccag ttctgaagca gttcgtaaag
    37741 gattaattat tctaaatatg actcttgatc atgccaactt acagggtatg cttgagcata
    37801 acccagcacg actgctcaaa ccagcaatgt ttggtgcatc gatgaataaa cctcgcgagc
    37861 gatggcttcc tgagaatgaa ctccggatgc tatggaaggc tttggatgat gctactgttg
    37921 gaggaggctc cattgcatct ggtggctatg gagtttcctc tagtgtggta ttatctcatt
    37981 ctgtcgcaaa tgcactccgt ttgattattt tcacaggagt gcgccgctct gaagcggcac
    38041 aaatgcgctg ggatcaaatt aatggagagc gttggaccat accagccact aaaaatggga
    38101 aaagccatat tgttacttta catccgtata tgctttcttt aataaaaaca caacgtgaat
    38161 tcactgaagg tgcatatgtt tttggttcta ctagcaaacc tggctttccc atcacaaatg
    38221 acgctctgac tcgcgcgtta gaaagggttc gctctaagta tctagctgaa cttactccat
    38281 tttctcctca tgatctgcgt agaagtgttg cgactggatg cgctgaatac cttgatgctc
    38341 cagagcgcct aatagagtta ctgttaaatc atgtacccaa agaccgttta atacgaacat
    38401 atcaagtcgg acaacaagct gaaaaattaa aaaatttatt tatagaatgg gggaatttta
    38461 ttatgaataa cgtcattact gtgcagaaga atgctccgga taatgttatt catataaaat
    38521 ttgggaacag atgataaaaa tacatgcatg gtctgagata ccctgtctac tcgaccatgc
    38581 agtatggttc aactaaatca gtcacaacaa gaaatgatta gttttattag atattgtatg
    38641 ggattatttc tggataatac ttcgcaatga tgtcatttag ccgatcaatc agttccggtt
    38701 ttctgaacgt gatgtgcgcg gagcccttct taaagtatct gatgctgaac atctcatctt
    38761 catagcatgt tttaccctga acagactgga tgtgctcatc caaacgaatg atgatgtttt
    38821 cccggttgtc gggaatgggg ttgccactga agagatgcag catcctttcc aggtcggcaa
    38881 cccggtcagt ctgttgcccg gtgataagat gaacccccca ccggtcccac ctcaccaggt
    38941 tgttgacgat aattttactg ccaaatttgc aggggcaatt ggttttgtaa ttccagctca
    39001 gtcccctgaa gacgttgatc acccctcgct caaacacctc atccttattc tggtgtagtt
    39061 gttcgaatgt gctcagaatg ttcgcttcac tgatttccgg aaaattatcg tactccagtg
    39121 accggtacca tgtatcacgg gcctgtgcat ccattaagga aagtatccct gaacgttgca
    39181 tcaggtcacg ccagatttcg cggtcaaggt tccgggtgat ggctttcatt gccatttccg
    39241 gtttctccat cagccagcag ccgcagcgaa aatcctgttt catggcccag tccagtgcag
    39301 ttttacctcc gatgctttcc gtcagcactg aaatttcagc cagttgccgg ataagcattt
    39361 cggcttgttg cagtgctgca taccgtaccg tcacaacgtg tcggatactg gttgagccga
    39421 taacgtccgt atgcccgttg accacttccg gacgggagac tgtgatatca gacataatcg
    39481 atattccttt ttgcagataa atgaaaatgc cagacctgtt caggctggcg tcagtggaat
    39541 gaagtctggt gagtaagact caccggttct gttgcagaag ctgcaactgg cgcagggtat
    39601 ggatatcctc cctcgcctgt tcaatacagg gcagcagtgc tgccgaatcc gttttatcct
    39661 catcgttgtc gaagataatt ccggattcgc agtcgatatt gtcctgcagc cacgcaatca
    39721 gaatatccag tgcggtttcc atggttaatg atttcatgtt gtgcctggtt accagttgaa
    39781 agtgggtaac cccggcaggc attcagcgct ggcatccaga tgaatgagac tgacgccata
    39841 acggcggatg agaatgacaa ccagacgacg gaacgttctg gacaggccca gtcgtttaag
    39901 ccgcagaacc ggatacggcc acgcatcagt acgtaacaga tagccgctgc cggtaaaatg
    39961 aatccattct gattcaccga agtcactggt ctggtgtgac agcgagtaca gccaggcgtt
    40021 gtccgtttcc gtgatatgtg cggtactgca gcgtataccg gtaagggcag caaatggtgg
    40081 catgcggtct gttgaggcca ggtcagatgc atcaacaaca cgaagggagt ggccattatc
    40141 acagaccacg ttaatcagtt cagcgagatt aacaccatcg acgtcaacga caatgcgccc
    40201 catgttcagt gcctgtacgt taacgctgtc ggcttccagc gtcagggcca gtttcattgt
    40261 ttcgcccccg gatgtttacc ccgggtaatg ttatttaccg ttctgtaatt gtcgcgggtc
    40321 atcaggcctg tcgcccggcg ggcccggagg atatcgatac tgttgattaa ctgagagtgg
    40381 gtacaggcgc tgaatcccgg ctggtcggtg cgcaccagcg cgtatttttc aacgagaaag
    40441 ttcaccgcat cacacagtga aatccctgcc tcaatatgct gctcaatcac acgttcatca
    40501 gcgaacggtg tgtcattcag tgtgaggccg taatgctggt ccagcagtcg ggtgagcagt
    40561 gtctgccaga tggtgacggg agacgggcag cacgatgcct cccgtacgtg tgtgtcaggt
    40621 aatgttttca tcttaagttc tctgtaactg gtagtgctaa aggtgagagc ttcggggtta
    40681 tgaggtggtt gcgggtgccg gtgtcggata aacagccaga taaacgtaac cacaggagcc
    40741 gagggtgtcg gcttcgcagg tcagcccttt tgcatacagc gtgacggtat gctgatgtcg
    40801 gggattcagt tcaccgctgg tgagcatgag ttccagttgt ttcatcagca gcggaaaggc
    40861 ctggtccagg tggtacgcat ctgcatctct gaaccggcct ctgataccgg cgcggtcggc
    40921 aaggtaatgc aggcggttac cctcctgcac cagacgggcg ccaaaacagg gcctcactgt
    40981 gcagggaagc ccccaccagg ggcggtcgtg attgtcgtcg ggatgagtta tcccggagag
    41041 tttgtctgac acgataaaat cctctctgaa aagtggttaa gaatggttta acgatgacag
    41101 gtcagccggc gtggagaccg gcttcggtga agatgtattc attgatgaga agggcctcat
    41161 ccacagcggc gtctgaggtc atctggtcgt actcgttttc cgggtgacgg aaca
//
LOCUS       DBBGLQ010000038        41126 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.038, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000038 DBBGLQ010000000
VERSION     DBBGLQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.038"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..66
                     /locus_tag="ACIYSB_002621"
     ncRNA           <1..66
                     /ncRNA_class="other"
                     /locus_tag="ACIYSB_002621"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(250..927)
                     /gene="narI"
                     /locus_tag="ACIYSB_002622"
     CDS             complement(250..927)
                     /gene="narI"
                     /locus_tag="ACIYSB_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGC5429649.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(927..1637)
                     /gene="narJ"
                     /locus_tag="ACIYSB_002623"
     CDS             complement(927..1637)
                     /gene="narJ"
                     /locus_tag="ACIYSB_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGC5429650.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1634..3172)
                     /gene="narH"
                     /locus_tag="ACIYSB_002624"
     CDS             complement(1634..3172)
                     /gene="narH"
                     /locus_tag="ACIYSB_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGC5429651.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3169..6912)
                     /gene="narG"
                     /locus_tag="ACIYSB_002625"
     CDS             complement(3169..6912)
                     /gene="narG"
                     /locus_tag="ACIYSB_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGC5429652.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNQEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEVNDQVQESVK"
     gene            complement(7428..8819)
                     /gene="narK"
                     /locus_tag="ACIYSB_002626"
     CDS             complement(7428..8819)
                     /gene="narK"
                     /locus_tag="ACIYSB_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGC5429653.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9158..10954
                     /gene="narX"
                     /locus_tag="ACIYSB_002627"
     CDS             9158..10954
                     /gene="narX"
                     /locus_tag="ACIYSB_002627"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGC5429654.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10947..11597
                     /gene="narL"
                     /locus_tag="ACIYSB_002628"
     CDS             10947..11597
                     /gene="narL"
                     /locus_tag="ACIYSB_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGC5429655.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11598..12992)
                     /gene="ychO"
                     /locus_tag="ACIYSB_002629"
                     /gene_synonym="iatA"
     CDS             complement(11598..12992)
                     /gene="ychO"
                     /locus_tag="ACIYSB_002629"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGC5429656.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13177..13530
                     /gene="ychN"
                     /locus_tag="ACIYSB_002630"
     CDS             13177..13530
                     /gene="ychN"
                     /locus_tag="ACIYSB_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGC5429657.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13574..14269)
                     /gene="chaC"
                     /locus_tag="ACIYSB_002631"
     CDS             complement(13574..14269)
                     /gene="chaC"
                     /locus_tag="ACIYSB_002631"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGC5429658.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(14427..14657)
                     /gene="chaB"
                     /locus_tag="ACIYSB_002632"
     CDS             complement(14427..14657)
                     /gene="chaB"
                     /locus_tag="ACIYSB_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGC5429659.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14927..16027
                     /gene="chaA"
                     /locus_tag="ACIYSB_002633"
     CDS             14927..16027
                     /gene="chaA"
                     /locus_tag="ACIYSB_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGC5429660.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16431..16538
                     /locus_tag="ACIYSB_002634"
     CDS             16431..16538
                     /locus_tag="ACIYSB_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGC5429661.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            16966..17073
                     /locus_tag="ACIYSB_002635"
     CDS             16966..17073
                     /locus_tag="ACIYSB_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGC5429662.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(17222..18076)
                     /gene="kdsA"
                     /locus_tag="ACIYSB_002636"
     CDS             complement(17222..18076)
                     /gene="kdsA"
                     /locus_tag="ACIYSB_002636"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGC5429663.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(18112..18921)
                     /gene="sirB1"
                     /locus_tag="ACIYSB_002637"
     CDS             complement(18112..18921)
                     /gene="sirB1"
                     /locus_tag="ACIYSB_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGC5429664.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18925..19317)
                     /gene="sirB2"
                     /locus_tag="ACIYSB_002638"
     CDS             complement(18925..19317)
                     /gene="sirB2"
                     /locus_tag="ACIYSB_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGC5429665.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(19314..20147)
                     /gene="prmC"
                     /locus_tag="ACIYSB_002639"
     CDS             complement(19314..20147)
                     /gene="prmC"
                     /locus_tag="ACIYSB_002639"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGC5429666.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(20147..21229)
                     /gene="prfA"
                     /locus_tag="ACIYSB_002640"
     CDS             complement(20147..21229)
                     /gene="prfA"
                     /locus_tag="ACIYSB_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGC5429667.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(21271..22527)
                     /gene="hemA"
                     /locus_tag="ACIYSB_002641"
     CDS             complement(21271..22527)
                     /gene="hemA"
                     /locus_tag="ACIYSB_002641"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGC5429668.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22741..23364
                     /gene="lolB"
                     /locus_tag="ACIYSB_002642"
     CDS             22741..23364
                     /gene="lolB"
                     /locus_tag="ACIYSB_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGC5429669.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            23364..24215
                     /gene="ispE"
                     /locus_tag="ACIYSB_002643"
     CDS             23364..24215
                     /gene="ispE"
                     /locus_tag="ACIYSB_002643"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGC5429670.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            24366..25313
                     /gene="prs"
                     /locus_tag="ACIYSB_002644"
     CDS             24366..25313
                     /gene="prs"
                     /locus_tag="ACIYSB_002644"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGC5429671.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            25438..27117
                     /gene="dauA"
                     /locus_tag="ACIYSB_002645"
     CDS             25438..27117
                     /gene="dauA"
                     /locus_tag="ACIYSB_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGC5429672.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(27172..27450)
                     /gene="ychH"
                     /locus_tag="ACIYSB_002646"
     CDS             complement(27172..27450)
                     /gene="ychH"
                     /locus_tag="ACIYSB_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGC5429673.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27727..28311
                     /gene="pth"
                     /locus_tag="ACIYSB_002647"
     CDS             27727..28311
                     /gene="pth"
                     /locus_tag="ACIYSB_002647"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGC5429674.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28428..29519
                     /gene="ychF"
                     /locus_tag="ACIYSB_002648"
     CDS             28428..29519
                     /gene="ychF"
                     /locus_tag="ACIYSB_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGC5429675.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            30288..33155
                     /gene="ycgV"
                     /locus_tag="ACIYSB_002649"
     CDS             30288..33155
                     /gene="ycgV"
                     /locus_tag="ACIYSB_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5429676.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDAIINFTGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMTMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(33255..35174)
                     /gene="dhaR"
                     /locus_tag="ACIYSB_002650"
     CDS             complement(33255..35174)
                     /gene="dhaR"
                     /locus_tag="ACIYSB_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HGC5429677.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQVLWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            35402..36472
                     /gene="dhaK"
                     /locus_tag="ACIYSB_002651"
     CDS             35402..36472
                     /gene="dhaK"
                     /locus_tag="ACIYSB_002651"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HGC5429678.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            36483..37115
                     /gene="dhaL"
                     /locus_tag="ACIYSB_002652"
     CDS             36483..37115
                     /gene="dhaL"
                     /locus_tag="ACIYSB_002652"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HGC5429679.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            37126..38544
                     /gene="dhaM"
                     /locus_tag="ACIYSB_002653"
     CDS             37126..38544
                     /gene="dhaM"
                     /locus_tag="ACIYSB_002653"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGC5429680.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            38864..40561
                     /gene="treA"
                     /locus_tag="ACIYSB_002654"
     CDS             38864..40561
                     /gene="treA"
                     /locus_tag="ACIYSB_002654"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGC5429681.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(40640..41080)
                     /gene="ycgY"
                     /locus_tag="ACIYSB_002655"
     CDS             complement(40640..41080)
                     /gene="ycgY"
                     /locus_tag="ACIYSB_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HGC5429682.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
BASE COUNT        10164 a        10685 c        10299 g         9978 t
ORIGIN      
        1 cgttgccgca ggcaacgacc cgaagggcga agcgcgcagc gctgagtaat ccttccccca
       61 ccaccatcgc ttccttaaat aatcaaaacc tgttactccg agctaatttt gcccgatggc
      121 accacgttta ccgggcctac aatatcctcc cctgtaggtc ggataagcga agcgcatccg
      181 acaactcacc ccgcaccgtg cccataaaaa aaccccgccg aagcagggtc tatgtgaact
      241 aaaattcgct tagtgacgag cgcgcaccag ctggtactta cgtgtcagat actccaccgg
      301 tacgctccag atgtgtacca gacgcgagaa cgggaacagc aggaataacg tcatccccag
      361 caccaggtgc agacggaaga taaacgccac accatcgagg tgttgagaag cgccaccgtg
      421 gaaggtcacc accgactgcg cccagccaac cagtttcatc atctcgctac cgtccatatg
      481 ctgagcggag aacggaatgg tcaacaggcc cagcgcgcac tggataacga gcagcgacag
      541 gatcaggata tccgctccgg tagtggttgc acgcacgcgt gggctgaaca gacgacgttt
      601 cagcaacagc acgccgccaa tcagacacag cacgccgctg gcaccaccag caaacattgc
      661 cattttctgt ttcacttcaa tcggcagcca ggcttcatac atccagtgcg gcgtcagcat
      721 accgaagaag tgaccgacaa aaatccccag aatcccgata tggaacaggt tcgacgccag
      781 gttcatccct ttgcgatcca gcatctggct ggacgccgcg cgccaggtgt actgcccgta
      841 gtcataacgc agccagctac caatcaggaa gaccgccccg gctatgtacg ggtagatatc
      901 aaagaagaac atattcagga attgcattag tgctgtcctc cggtggtgat attcagatat
      961 tgcggcgcga cggcaccggc aaagcgacgc tgatgcgcag tgattgctga atcaccgcag
     1021 cctttgtcag caaagaattt aacctgctct tcttcccaga cagcatccag cgcctgcggc
     1081 gtatcatcgc gcgcttcgtc ggcaattttt tccgccactt tgtcgctgtc gatagcggta
     1141 ttcgccagtt tcagcagcag atcaaacagc acggcataac ggctttcacg ctgttgcaga
     1201 cgcgcgctca gcaatgccag aatcggcgcg atatctttca aaccttccac ggcttcgctt
     1261 tgcggcagct gcgccaggta ctccagatac agcggcagat ggtccggcaa ttcgcggctg
     1321 tttaactgca agccgtgctg ctcgtactgc gccagcaggt ccaccatcgc ctggccgcgg
     1381 tcgcgggatt cgccgtgcac atgttcaaac agcaacagtg acgtggcgcg gccacggtcg
     1441 aacagttcgc tgtactgcgc ctgggcatcg agcggatcca tcgtcgttaa atcgcgcagg
     1501 aaaatgccca gcgcatgggc atcctctttt ggcagatttt tcgacgcggc aatcgcctca
     1561 aacatctctt gttgatgctg ccataaggca gcatccggat attcaaggag acgcgataca
     1621 atcacgagtt cgatcatgga tgcggctccg ttttgctggt cacatcgatg gcatcgatac
     1681 gacggctgtt gaacagattg aatttggtat ctgaaccgtg gcagccatca ccaaaggtaa
     1741 agccgcagcc atttttctcc gggaaggctt cccgtgccag ttcacgatga ctactcggca
     1801 ccacaaagcg atcttcgtag ttagcaatcg ccagataacg gtacatctcc tgtgcctggg
     1861 cttcggtcag accgacctct tccagcgcac gggtatcaac tttaccgtca acggtttcag
     1921 cacgtttgta atgacgcatc gccagcatac gtttcagtgc gcgcagtacc ggtttggtat
     1981 caccggcggt cagcagattc gccagatact gtaccggaat acgcaggctt tcgacgtctg
     2041 gcagaatgcc gttgctaccc agctcacccg cgtctgctgc agactgaatc ggagacagag
     2101 gcggcacgta ccagaccatc ggcagtgtgc gatattccgg atgcagcggc agcgccagtt
     2161 tccattccat tgccatttta taaaccggcg actgctgtgc ggcttcaata acgctcagcg
     2221 gaataccgtc tttaatcgcc tgctcgatga ctttcggatc gttcggatcg aggaacacgt
     2281 ccagctgacg ctggtaaaga tctttctcgt tctcggtgct ggctgcacgt tcaatagcgt
     2341 cggcatcgta caacagcacg ccaagataac ggatacgacc gacacaggtt tctgagcaca
     2401 cggtcggctg acccgcttca atacgcggat agcagaagat gcacttctca gacttaccgc
     2461 tcttccagtt gaagtagatt tttttgtacg ggcatccagt gatgcacata cgccagccac
     2521 ggcatttatc ctggtcgatc aggacgatgc catcttcttc acgcttgtaa atcgcaccgc
     2581 tcgggcaggt cgccacacat gccgggttca ggcagtgttc gcacaggcgc ggcaaataca
     2641 tcatgaaggt gttttcgaac tggctataca tcgccttctg gatgttgtcg aagttcttgt
     2701 ctttcgccag tttgtcaaac tcaccgccca ggtcatcttc ccagttcggc cctttttcga
     2761 ttttcgccat ccgttccccg gtaatcagcg aacgcggacg ggcaatcggc tgcgatttgc
     2821 tgccttccgg cgcggtatgc aggttctgat agtcaaaatc gaacggctcg taataatcgt
     2881 cgatccccgg cagatgcggg ttagcgaaga ttttacccag cagcatggca cggttaccca
     2941 tgcgcggctg cagtttgccg ttgattttac ggatccagcc gcctttgtat ttttcctggt
     3001 tttcccagtc agtcgggaag ccctggcccg gcttggtttc cacgttgttg aaccacgcat
     3061 attccacgcc ttcacggctg gtccagacgt ttttacaggt aactgaacag gtgtggcagc
     3121 cgatgcactt atcgagattc agcaccatgc cgacttgtga acgaattttc attttacgct
     3181 ctcctgtacc tggtcattga cttcgccatc taaccagtca atgttcttca tcttacgcac
     3241 tacaacaaac tcatcgcggt tagaacctac ggtgccatag tagttaaagc cgtatgccag
     3301 atgggcatag ccgccgatca tatgcgtcgg tttcggcgtg atacgggtga ccgagttatg
     3361 gataccacca cgctgttggg taatttccga accaggcagg ttaacgatac gttcctgcgc
     3421 gtggtacatc atggtcatcc ctgccggaac acgctggctg acaaccgcac gggcagtcag
     3481 agcaccgttg ctgttgaaga cttcaatcca gtcgttatcg gcgataccca gatctttggc
     3541 atcggcttca ctcaaccaga ccaccggacc accgcgacct aaagtcagca tcagcaggtt
     3601 gtcgctgtag gtggagtgga taccccactt ctggtgcggc gtcaggaagt tgagcgcttt
     3661 ttcctggttg ccgttggatt tctggcctat cacttctttc accgaacggg tgtcgatcgg
     3721 cggacgataa accagcaggc tttcaccgaa atcacgcatc cactggtgat cctgatacag
     3781 ttgctgacga ccagagagcg tacgccatgg gatcagctcg tgaacgttgg tgtaaccggc
     3841 gttgtaagaa acgtgttcat cttccagacc agaccaggtc gggctggaga taattttgcg
     3901 cggctgtgcc tgaatatcgc ggaagcggat cttctcgtct tctttattca gcgccagatg
     3961 cgtatggtca cgaccggtaa attcgctcag ggcagcccag gctttcacgg ctacctgacc
     4021 gttggtttcc ggtgccagtg tcaggatcat ctctgccgca tcaattgcgg tgttcagcat
     4081 cggctggcct ttcgccggac cttccgcttt ggtgtagttg agcttacgca gcagatccat
     4141 ctcgctctgg gtgttccagg caatcccttt accgccatta ccgattttct ccatcagcgg
     4201 gccgatagag gtaaagcgtt cgtaagtcgc cggataatcg cgctctacga ccataatgtg
     4261 tggcgcggtt ttacctggga tcaggtcgca ctcgcctttt ttccagtctt tcacatccag
     4321 cggctgcgcc agttcagcgg cagagtcatg ctggataggc agcgtgacga tgtcggtttc
     4381 tttacccaga tggccgacgc acacttcgga gaatttcttc gcgatggctt tgtagatttc
     4441 ccagtcgctt ttcgcttccc aggccggatc gaccgccgca gacagcgggt gaataaacgg
     4501 atgcatatcc gaagtattca tgtcgtcttt ttcgtaccag gtcgccgtcg gcaaaatgat
     4561 gtcggaatag agacaggtgc tcgacagacg gaagtccagc gtaaccacca gatccagctt
     4621 gccttccaga ccattgtcct gccagtccac ttcttccggc ttcacgccgc cctgttgccc
     4681 cagatcttta ccctggatac cgtgctccgt ccccagcagg tacttgagca taaactcatg
     4741 acctttaccg gaagaaccga gcaggttaga acgccagatg aacaggttgc gcgggtggtt
     4801 tttaccgttt tctggttgtt ctgccgcaaa acggatggaa ccctctttca gggatttcac
     4861 cgtatagtcc accggattca tcccggcttt ttccgcttcg ccagcgatag tcagcgggtt
     4921 agtgcctaac tgcggtgcag acggcagcca gcccatgcgt tccgcacgga cgttaaagtc
     4981 gatcaagtgt ccggtatagc gggatttgtc cgccatcggt gacagcaact cttccgccgt
     5041 gacggtttca taacgccact ggctggagtg gttatagaaa taagaagtgc tgttcatgtg
     5101 acgcgccgga cgctgccagt caagggcaaa cgccagcggc tgccagccgg tttgcggacg
     5161 cagtttttcc tgacctacat agtgcgccca gccgcccccg ctctgaccga cacagccgca
     5221 gaaaatcagc atgttgatca gaccacgata gttcatatcg aggtgatacc agtggttcag
     5281 ccccgcaccg acgataatca tcgaacgacc gtgcgtttta tcagcgttat cggcaaattc
     5341 acgggcgatg cgaataatct ggctgcgaga aacgccggta atctgctcgg cccaggccgg
     5401 ggtataagct ttcacatcgt catagctggt tgcacagtta acgtcgttca ggccacgttc
     5461 cagaccgtag tttgccagcg tcagatcata aacggtggtc accagggcgg tgctgccatc
     5521 agccagttgc aggcgtttca ccggcagttt gtgcagcagc acgttttcca gttccacttt
     5581 gttgaagtgt tccgtgccgt cgccaccaaa gtacgggaag cctacctcag cgatctcatc
     5641 ctggctaccc agcaggctca gttgcagctc ggtttcttcg ccagttttgc cgtcgcgctg
     5701 ctcaagattc catttgccct tctcgcccca gcggaagcca atagaaccgt tcggcgcaac
     5761 catttcgcca ttggtattaa aggcgacagt tttccattcc ggattgtttt cctggcccag
     5821 cgcatcaacc agatcagcag cgcgcagcat acgacctgca gcgtagtaac cgtcgcgttc
     5881 ttccagcatc accagcatcg gcatgtcggt gtagcgacgc acatagtcgg tgaaatactg
     5941 gcttgggttg tcgaggtgga attcacgcag cattacgtgg cccatcgcca gcgccattgc
     6001 cgcatcggtg ccctgtttcg gtgccagcca cagatcgcac agtttggcga tttcagcgta
     6061 gtctggtgtg acggcaacag ttttggtccc tttgtaacgc acttcagtaa agaagtgagc
     6121 atccggggta cgcgtctgcg gcacgtttga cccccaggcg atgatgtagc tggagttgta
     6181 ccagtcagca gattccggta cgtcagtttg ctcgccccag gtttgcggag acgcaggagg
     6241 caagtcgcag taccagtcgt agaagcttaa gcaagtaccg ccaatcagcg agagatagcg
     6301 tgcacccgat gcgtaagaaa ccatcgacat tgccggaatt ggcgagaaac cagcaacacg
     6361 gtccgggccg tagtttttga tggtgtaaac gttagatgcg gcgatcagtt cgttcacctc
     6421 ctgccaggaa gaacgaacaa atccaccgcg tccacgcgcc tgcttaaagc ttttcgcttt
     6481 atcggcgtct tcaatgatag aagcccatgc ctcaaccgga tcgctatgca gcgccttcgc
     6541 ttcacgccac attttcatca ggcgtttgcg catcatcggg tatttcaggc ggttggcact
     6601 gtaaagatac caggagtagc tggcaccgcg cgggcagccg cgaggttcat ggtttggcag
     6661 atccggacgg gtacgcggat agtcagtctg ctgggtttcc caggtgacca gaccgttttt
     6721 gacgtagatt ttccagctgc aggagccggt gcagtttacc ccgtgggtag agcggacgat
     6781 tttgtcatgc tgccaacgct ggcgatatcc atcctcccag tcacggttgg tattgagaag
     6841 ctggccatgc ccatcggcaa aggtttcacc cttctgtttg aagtagcgaa accggtccag
     6901 gaatttactc atcggttttc tcctgtggga gcctgtcggc cctctgataa atcgacattg
     6961 ctaatgttta agggcaaggt aacgctctga aacagcgtgg gaattgataa cgatcaagag
     7021 tgatggggat gaaaaataaa gtaaattcct aagatacccc tttaggataa ttaacagcca
     7081 ccacataaca gactgtaaat taagacgttt tatacctgta gttatttaaa ctattaagat
     7141 ttttatgcag atgggtatta aggagtattc cccatgggta acatattaat ggctcttaat
     7201 gaaatccggc gaaataaacc atataacatt agtatgttgt aactaaatga cgcataaaaa
     7261 aagcgcgaca tcatgccgcg caaaggataa tcaaatattt tactctatat aattcggttt
     7321 acaggaaggc ggcaagtgaa tgaatcccag gagcttacta aagtaagtga ctggggtgaa
     7381 tgaacgcagc cgcagcacat gtaacccgaa gtatgacgag taacggttta ttttttagaa
     7441 tgccgaccat ataccgccca ggtaatcact acgcaggcga tatagaaaat caaaaatacc
     7501 ttcattgcgc cgactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
     7561 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgccgc cgtgtcggtt
     7621 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
     7681 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
     7741 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
     7801 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
     7861 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
     7921 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
     7981 agcattgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
     8041 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
     8101 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
     8161 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
     8221 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
     8281 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
     8341 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
     8401 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
     8461 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
     8521 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
     8581 agcgcagtca acataaatag ctgatcggtc gtaaaattaa agccgacttt cggtaggttc
     8641 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
     8701 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
     8761 ggtcgccaat ctgtaatgac agctccagta gccctttcgg gggcggatga gtgactcata
     8821 gacacctctg atactcgttt cgatgtctgc caccttagtg tctgtagcta aaggcaattt
     8881 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
     8941 gagtacagtg acttcataaa aattatgaga tttttcacgg tgctgtaaaa tccctaccct
     9001 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc gggtatgggt
     9061 atacttcagc caatagccga gaatactgcc attccagaat gtatcgtcac attcattaag
     9121 gttattgctc atttaaagcc tgaaggaaga ggtttacatg cttaaacgtt gtctctctcc
     9181 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
     9241 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
     9301 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
     9361 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
     9421 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
     9481 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
     9541 ccagtttgtt gccgggcttg atcaactggt atctggtttt gaccgcacca cggaaatgcg
     9601 catcgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
     9661 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
     9721 ggcgagtgcc gtcagtcatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
     9781 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
     9841 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
     9901 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
     9961 gtcacctgta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aattgcgggt
    10021 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    10081 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    10141 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    10201 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    10261 actcaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    10321 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    10381 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    10441 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    10501 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaggcgag
    10561 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    10621 tcgcctggtg ccttcgcatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    10681 taacgccctc aaacattcgc aagcgagtga agtcgtggtg acggtggcgc aaaacgataa
    10741 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    10801 caatcactac ggcatgataa taatgcgcga tcgtgcgcaa agtttacgag gcgattgccg
    10861 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    10921 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    10981 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    11041 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gagtctcttg
    11101 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    11161 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    11221 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    11281 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    11341 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    11401 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    11461 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    11521 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    11581 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    11641 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    11701 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    11761 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    11821 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    11881 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    11941 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    12001 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    12061 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttcaccctgt
    12121 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    12181 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    12241 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    12301 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    12361 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    12421 cccgctcgct gaagattttc gtccagcaag ttgtcataaa aagtgttata acccaccagc
    12481 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    12541 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    12601 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    12661 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    12721 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    12781 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    12841 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    12901 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    12961 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    13021 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    13081 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    13141 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    13201 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    13261 cgagagcagg agagcaatct ggatctgcgt ctgttcctca tgtctgatgc ggtcacagcc
    13321 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    13381 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagt
    13441 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    13501 ctgtcagccg ataaagtgct cacattttaa tgtttgaggg ggcgtctgac gccccactat
    13561 gcgggaagtt tactcaggcg aatcccggac gtaacacacc atccggaaaa ttctccgcca
    13621 gcagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    13681 taagctcctg ttccagtgaa aacaggtatt gtgcgttggt tcccagcgga ccgctcgccg
    13741 ccgcaatcaa cggcgcgatg acctgagcgc gagtatcaga ttcatattct ggatgtcgcg
    13801 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    13861 aggttggcag ataacagccg gtaatcatct ctcgcttcca caacagggtt agttcctgct
    13921 ccagcgtctc ttctggcagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    13981 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaatg
    14041 cgcgatgcca tccaaccagt gtaccggtgc acgattcggt aaactccagt gccggattcc
    14101 acatcaacga tccatagccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    14161 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    14221 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    14281 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt tactgctcat
    14341 tttgaaacca ctaacgggac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    14401 gaatagcttt aactaagcga ccggttttac gattttttat gccatttatc atcatcccct
    14461 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    14521 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    14581 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    14641 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    14701 atatctactc ttgccaaaac ataagaattt tcaggtcaga ctcttcttaa gaaacgctaa
    14761 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    14821 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    14881 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    14941 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggcgctggt
    15001 agtgctgttc ctgtggggaa gcagccagac actaccagtg gtcattgcca tcaatcttct
    15061 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    15121 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    15181 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    15241 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    15301 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    15361 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    15421 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    15481 tttgctgatc cagaccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    15541 tgatgatgac gacccgcatc acggtaaacc gtctgcccat agcagcctgt ggcatgctat
    15601 ctggttgatt atccatctga ttgccgttat tgcggtgacc aaaatgaacg ccagctcgct
    15661 ggagacattg ctcgacagca tgaatgcccc tgtcgccttt actggcttcc tggtggcact
    15721 gttgattctg tcgccggaag gtttaggtgc attaaaagca gtgttgaaca accaggttca
    15781 gcgcgcgatg aatctgttct ttggttcagt gttagcaacc atttcgctta ccgtacctgt
    15841 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    15901 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    15961 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    16021 tgcctgacag ttatatctcc tccgatgacg gtaatcgggg gagataatat aaattcctgc
    16081 ttattgtatt tcttcatact acctggacag gtcgttaatc agaattgcga gcctgtacca
    16141 caccctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc ttcattgcaa
    16201 acatttcagg gatgaagtga agatgaattg cgacgtagta tccacaccaa gccggcgtta
    16261 tccggtgagg cgcaatgttg cggggcttta tccctggtgg cattggttgc tggaaagaga
    16321 aaacccccgc acgttgagag gtaaaacctg acaacaaaac gggggctaat cttgaaacca
    16381 gacacttcaa gaatagccgc aaaatgttgt cattacaaca aggcggctat atgacgctcg
    16441 cgcagtttgc catgattttc tggcacgacc tggcagcacc gatcctggcg ggaattatta
    16501 ccgcagcgat tgtcagctgg tggcgtaacc ggaagtaagg tgaaaacggg cgtcaggctg
    16561 ccgtgatggc aatatgcgcc cgaaaccggc cgcaggggta acagcctgcg gccatttttg
    16621 tctttatctc ttccacaagt aaatattggc aacccgagct ggaagcatca tccagagcac
    16681 tcatgaaacg actgcaacag ctgttgaaaa gccggaatgt actctttatt gcaaacattt
    16741 cagggatgaa gtgaagatga attgagacgt agtatccaca ccaagccgga attatccggt
    16801 gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggaa agagaaaacc
    16861 cccgcacgtt gagaggtaaa acctgacaac agaacggggg ctaatcttga aaccagacac
    16921 ttcaagaata gccgcaaaat gttgtcatta caacaaggcg gctatatgac gctcgcgcag
    16981 tttgccatga ctttctggca cgacctggcg gcaccgatcc tggcgggaat tattaccgca
    17041 gcgattgtcg gctggtggcg taaccggaag taaggtgaaa acgggcgtca ggctgccgtg
    17101 atggcaatat gcgcccgaaa ccggccgcag gggtaacagc ctgcggcctt ttttgtatcc
    17161 ggcacccatg aaaaaagtct taacgcagaa cgctaatact ttatttttca agcaaaaaag
    17221 attacttgct ggtatccagt tcttcgaaac ctttcaccag atcatcaatc gctttcatct
    17281 gcttgaggaa cggttccagt ttagccagcg gcagcgcgga tggaccatca catttcgcat
    17341 gttccggatc cggatgcgct tcaataaaca gccccgccag acctaccgcc ataccggctc
    17401 gtgccagctc agccacctga gcacgacgac caccggaagc tgcgccaaac ggatcgcggc
    17461 attgcagtgc gtgggtcacg tcgaaaatca ccggcgagtt accagacact ttcttcataa
    17521 tgctgaagcc cagcatatcg acaaccaggt tgtcatagcc gaagttagca ccgcgatcgc
    17581 aaagaatcac tttttcgttg ccgccttctt tgaatttatc aacgatatta cccatctgtc
    17641 ccgggctgac aaactgtggt ttcttgacgt taattaccgc accggttttc gccatggctt
    17701 caaccaggtc agtctggcga gcaagaaacg ccggcaactg aatcacatcc acgacatcag
    17761 caacgggctg tgcctgactt ggttcgtgaa cgtcggtgat aattttcacg ccaaaagtct
    17821 gcttcaactc ctggaagatt ttcatccctt cttccaggcc cggtccacga taagagtgga
    17881 tggaggagcg gttggctttg tcaaaagagg ctttgaacac gtaagggata cccagtttct
    17941 gggtcacagt tacgtagtgc tcgcaaatgc gcatcgccag atcgcgagat tccaacacgt
    18001 tcataccgcc aaacagtacg aacggcaggt catttgctac gttgatgtcg ccaatgctaa
    18061 ccactttttg tttcatagga tcgccttaat cttgagtaaa atgtcgatta attaatgcag
    18121 cacaatatgt ttatgcgcga tgttatttat ttgcgcacgg atcatttcgc tgatcgggtc
    18181 ttccggacac tgttcaacga aataacttaa atcgttcaac gcaacgtgtt cgcaatccag
    18241 ttgcgcataa atcaacccgc gatcgcgaat ttcataggga tcttcagggt tgaattgtaa
    18301 taaagcttcg ctggtgcgta acgccagctc catctgattt tcttccatca acgaggcttt
    18361 gagtgtatcc agcaatttgc ggattacctc aatgttatca gcttcatcaa ggtcttcata
    18421 aaacagttcc gccgacgggc tgatatttcc ctttaaccat acgtccagca tatgttcgct
    18481 taacgattca ccgttaaaag gattaatcag ccaaatttcg ccatccggac attcaatgcg
    18541 caatatcagc tgcgtaggga aaatcaccgg cagcagcggc aaatcgagac gattcgcgac
    18601 ccataataaa accgcaccta atgataccgc actgccctgt cgattcttta acacctggtc
    18661 cagccataat gcatcggaaa gacgataaac accgcgtgag gctttaaatc cccagtcgcc
    18721 gtaaaacagc gcgatcagtt tttccaactg ctcttctaaa ggcagaagct ggctgatttc
    18781 ttccttcgcc agactaacga gacgctccag ttcgtcgtaa acatcttgcg agggaaaatc
    18841 gcggcggatt gcttcgcaag ccaggatcat gccttcgcac aatggcgctt tattaaattc
    18901 gaaatcagct aacgatctca tgacttaccc cagtaacggt acttttgtgg tggcgagttt
    18961 aatgatgatg tacagcacca ccaacgccag cgggaaggca ataatgcgcg cctgctgact
    19021 gtgcatacga cgataatcga gtgcaataaa acccaaaacg atataaatga taactccaaa
    19081 cagcttttca gtcagccact gtgcctgccc ggaaaatggc aggatgtgcg ctttagccat
    19141 caacgcaatg ccgcttaaca gtaacagcgt atcgacaacc ggcggcacaa tgcgcgtcca
    19201 gcgagcaaat gcctgtggat gcttctgata acgtagccag aaacgtaagg ttaatagccc
    19261 aacagaaagc gcgatactaa taagatgaac actaagcagt gtagaaaaac ttgtcattga
    19321 taatagcggc cgagcgttac gcgctcgtta tcaccatagt cacggcaggt ttcgacgtca
    19381 tgataccccg cgaggataaa tgcttgtcgc accgcttcgc cctgctgcca gccatgttcc
    19441 agaagcagaa agccgccgga taccagcgcg ttacgcgact gttcgatgat atgcacgatg
    19501 tctgccattc cactgtctgc cgcaaccagc gcagtgagcg gctcaaagcg gacatcgcct
    19561 tgttgaagat gagggtcctg ctcgtcaata tacggcggat tgctgacaat catcgcaaac
    19621 tgctgcccgg ctagcgcgct aaaccagtcg ctttgcagaa tgtggatatt tttgatcgcc
    19681 agatgctggg cattacgttg tgccagggag acagcatcag gcatacgatc tacagcgata
    19741 atttcgcagt ccgggcgctc gctagccagc gcaagcgcaa tcgccccggt acccgtcccg
    19801 agatcgagaa tacggcaagg ttgttcaggc aaccgcgcca gtgcctgctc caccagacac
    19861 tccgtatccg ggcgcggaat taaggtcgct ggcgaaacaa ataacggcaa cgaccagaat
    19921 tctcgcaccc cggttaaatg agcaatgggt tcaccatcgc gacgacgtgt cagtagcgca
    19981 tcaagttgct gacattgttc gtcagtcagc tgcgtttcac caaaggcgag aataaaagta
    20041 cgccctctgc cggtaacatg ctccagcagg atttcagcat cacgccgcgg gctttcgctc
    20101 gcctgaagtt ggcttattgc ttcacgtaac cagtgttgat attccattat tcctgctcgg
    20161 acaacgccgc cagttggtcg gcctgatgtt cctggataat cggttcaatc agcatatcca
    20221 gcttaccttc catcacttca tccaggcggt agagcgtcag gttgatgcgg tgatcggtaa
    20281 cgcgcccctg cgggaagttg taagtacggt tacggtcgct gcgatcgcca ctccccagca
    20341 ggttacgacg ggtagacgct tcggcctgtt ggcgttttgc catttcagca gcgtggatgc
    20401 gagcaccgag aacagaaagt gctttagctt tgtttttatg ttgtgaacgt tcgtcctgac
    20461 attcaacaac aatcccggtc ggcaagtgag taatacgaat tgccgaatcg gtggtgttaa
    20521 cgtgctgacc acccgcccct gacgagcgga aagtatcaat gcgtaaatct gctgggttga
    20581 tgtccggcag ttctgcgtca ggcagttctg gcattaccgc aacggtacaa gcagaagtat
    20641 gaatacgacc ctgcgattcc gtagcaggaa cacgttgcac gcgatgaccg ccggattcaa
    20701 atttcagacg accatacaca ccatcaccgc taattttggc gatgatctct ttataaccac
    20761 catgttcacc ctcgctggcg ctcatgattt ctacccgcca gcggcgggct tcggcataac
    20821 ggctgtacat acggaacaga tcgcccgcga acagcgccgc ttcgtcgccg ccggttccgg
    20881 ctcggacttc gaggaaggcg ttacgttcgt catcaggatc ttttggcagt aacagaacct
    20941 gtaattgctg ttccagttgc tcgctttttt ctttagcttc gcgcagttca tcctgcgcca
    21001 tctcacgcat ttcaggatca tcgagcatca tctgtgcggt ttcgatatct tcctgaacct
    21061 gttgccagtc ggtaaaacag cgcgaaacat cacttaactg cgcatattcg cgtgataatg
    21121 cgcgaaaacg ttcctggtcg gcgatagttt gcgcgtcacc cagcaacgcc tgaacttctt
    21181 catggcgttc atgcagggct tccagtttgg caacgataga aggcttcata ggcgtaaatg
    21241 caccctgtaa aaaaagaaaa tgatgtactg ctactccagc ccgaggctgt cgcgcagaat
    21301 attcaggcgt tcgttatccc cgtcacgggc ggcctgttga agtgatttcg ttggcgcatg
    21361 gatcaagcgg ttagtcagtt tccatgccag atcctgcata atggcttgcg cgtcgccgcc
    21421 ctgctcaagg gccgctaacg ctttggcggt taactcatcg cgaacttgct ctgcctggct
    21481 gcgatactcg cgaatggttt cgctggcgct ttgtgctcgc agccacgcca taaattcgct
    21541 ggtttcctga gcgacaatag tttccgcctc aaccgctgcg gctttacgct gcgccaggtt
    21601 gtgcgaaatg atgctttgca gatcatcaac gctataaaga taagcattcg ccagtttgcc
    21661 aacttccggc tcaacatcgc gcggaacggc aatatccacc aacagcattg gttggttgcg
    21721 acggcttttt aatgcgcgct ccaccatgcc tttcccgata atcggtaacg ggctggcggt
    21781 ggaactgatg atgatatcgg cttcgcgcag acgttcgtcg atatcactca gggcaatcac
    21841 ttccgcgccg acttcatctg ccagaatttg ggcacgttcg cgagtgcggt tggcgataat
    21901 catcttctgt actttgtgtt cgcgcagatg acgcgccacc agctcgatag tttcgcccgc
    21961 gcctaccagc aacactgtga ccgtagagag cgattcaaag atctgccgcg ccagcgtaca
    22021 agccgcaaaa gcgacagaca cagcgctggc accgatatct gtttcagtgc gaacgcgttt
    22081 cgcgacagag aaagatttct ggaacatgcg ttccagttcg ctggccttca tatgaccttt
    22141 ttgcgaatcg gcaaacgctt ttttaacctg accgaggatc tgcggctccc ccagaaccag
    22201 tgaatccagg ccgctggcaa cacgcattaa atggctaacc gcgtcgttat cctgatgcca
    22261 gtagaggctt ttacgcagat cttcttcatt aagattgtga taatcgcaaa gccagcggat
    22321 taacgcctct tgcaggttgt cctgctcttc aacgctaaga taaagttccg tgcggttgca
    22381 cgtcgacagc accacgccgc cctgcaccat cggctgcgca agcaggctgt caagcgcctg
    22441 atcgagctta tccggcgaaa acgatacacg ttctcgcagc gatacaggtg ccgttttatg
    22501 gttgataccg agtgctaaaa gggtcatgtc tgcgggaaat aataccaacg ttgatagggt
    22561 tagtctgctt gcatcataca ggatgcgtaa gatcaataaa agagagcgcc cccttttgga
    22621 gtaattgccg aaagccgtta gattctggca attaagacaa cttgaacata gacgatagcg
    22681 gacggtaacg ctagcattaa gggttataac tgcaacgtat ctcaaggact tgtcatcact
    22741 atgcccctgc ccgattttcg tcttatccgc ctgctaccgc tggctgctct tgtgctcact
    22801 gcctgttccg ttaccacgcc caaaggtcct ggcaaaagcc cggattcgcc acaatggcgt
    22861 cagcatcagc aagacgtgcg caatcttaat cagtatcaga ctcgcggcgc gttcgcttat
    22921 atttctgacc aacaaaaagt gtacgcccgc tttttctggc agcaaaccgg ccaggatcgc
    22981 taccgtctgc tgctcactaa cccattgggc agcacggaac tggagctgaa tgctcaaccg
    23041 ggtaacgtgc agttagtcga caataaaggt cagcgttata ccgccgatga cgccgaagag
    23101 atgattggca aattgaccgg aatgccaatt ccgctcaaca gcttgcgcca gtggatttta
    23161 ggtttaccgg gtgatgcaac cgactacaaa ctggacgacc agtaccgcct gagcgaaatt
    23221 acctacagcc agaatggcaa aaactggaag gttgtttatg gtggttatga caccaaaacg
    23281 caacctgcga tgccagccaa tatggaactc accgacggtg gtcaacgcat caagttaaaa
    23341 atggataact ggatagtgaa ataatgcgga cacagtggcc ctctccggca aaacttaatc
    23401 tgtttttata cattaccggt cagcgtgcgg atggttacca cacgctgcaa acgctgtttc
    23461 agtttcttga ttacggcgac accatcagca ttgagcttcg tgacgatggg gatattcgtc
    23521 tgttaacgcc cgttgaaggc gtggaacatg aagataacct gatcgttcgc gcagcgcgat
    23581 tgttgatgaa aactgcggca gacagcgggc gtcttccgac gggaagcggt gcgaatatca
    23641 gcattgacaa gcgtttgccg atgggcggcg gtctcggcgg tggttcatcc aatgccgcga
    23701 cggtcctggt ggcattaaat catctctggc aatgcgggct aagcatggat gagctggcgg
    23761 aaatggggct gacgctgggc gcagatgttc ctgtctttgt tcgggggcat gccgcgtttg
    23821 ccgaaggcgt tggtgaaata ctaacgccgg tggatccgcc agagaagtgg tatctggtgg
    23881 cgcaccctgg tgtaagtatt ccgactccgg tgatttttaa agatcctgaa ctcccgcgca
    23941 atacgccaaa aaggtcaata gaaacgttgc taaaatgtga attcagcaat gattgcgagg
    24001 ttatcgcaag aaaacgtttt cgcgaggttg atgcggtgct ttcctggctg ttagaatacg
    24061 ccccgtcgcg cctgactggg acaggggcct gtgtctttgc tgaatttgat acagagtctg
    24121 aagcccgcca ggtgctagag caagccccgg aatggctcaa tggctttgtg gcgaaaggcg
    24181 ctaatctttc cccattgcac agagccatgc tttaagccgg gcaagctgag tttcggtgac
    24241 aacgtcacct tgttccagac gttgcatcgc gctctttaat acaccgcctg gaaaggatca
    24301 tgcctggccc gcacagtttt cggcagattc tttccaccaa tggacgcatg cctgaggttc
    24361 ttctcgtgcc tgatatgaag ctttttgctg gtaacgccac cccggaacta gcacaacgta
    24421 ttgccaaccg cctgtacact tcactcggcg acgccgctgt aggtcgcttt agcgatggcg
    24481 aagtcagcgt acaaattaat gaaaatgtac gcggtggtga tattttcatc atccagtcca
    24541 cttgtgcccc tactaacgac aacctgatgg aattagtcgt tatggttgat gccctgcgtc
    24601 gtgcttccgc aggtcgtatc accgctgtta tcccctactt tggctatgcg cgccaggacc
    24661 gtcgcgtccg ttccgctcgt gtaccaatca ctgcgaaagt ggttgcagac ttcctctcca
    24721 gcgtcggtgt tgaccgtgtg ctgacagtgg atctgcacgc tgaacagatt cagggtttct
    24781 tcgacgttcc ggttgataac gtatttggta gcccgatcct gctggaagac atgctgcagc
    24841 tgaatctgga taacccaatt gtggtttctc cggacatcgg cggcgttgtg cgtgcccgcg
    24901 ctatcgctaa gctgctgaac gataccgata tggcaatcat cgacaaacgt cgtccgcgtg
    24961 cgaacgtttc acaggtgatg catatcatcg gtgacgttgc aggtcgtgac tgcgtactgg
    25021 tcgatgatat gatcgacact ggcggtacgc tgtgtaaagc tgctgaagct ctgaaagaac
    25081 gtggtgctaa acgtgtattt gcgtacgcga ctcacccgat cttctctggc aacgcggcga
    25141 acaacctgcg taactctgta attgatgaag tcgttgtctg cgataccatt ccgctgagcg
    25201 atgaaatcaa atcactgccg aacgtgcgta ctctgaccct gtcaggtatg ctggccgaag
    25261 cgattcgtcg tatcagcaac gaagaatcga tctctgccat gttcgaacac taatcgaacc
    25321 cggctcaaag acccgctgcg gcgggttttt ttgtctgtaa tatccatttg tatgacctat
    25381 gcctccttca cctgccattt agttgacaga tgatgcgctc acggatgaaa cattattgtg
    25441 aacaaaatat tttcctcaca tgtgatgcct ttccgcgctc tgatcgacgc ttgctggaaa
    25501 gaaaaatata ctgccgcacg gtttacccgt gacctgattg ccgggataac cgtcgggatt
    25561 attgctatcc cgctggcgat ggcgttggct attggtagtg gtgtggcacc ccagtacggt
    25621 ttatataccg cagctgttgc ggggattgtc attgctctga cgggtgggtc acgctttagc
    25681 gtttccggtc cgactgcggc atttgtggta attctctatc ccgtgtcgca acagtttgga
    25741 ctggcaggac tgctggttgc gaccttgctg tcggggatct ttttgattct gatgggtctg
    25801 gcacgctttg gtcgcctgat tgagtatatt ccggtttccg tcaccttagg tttcacctcg
    25861 ggtatcggga tcaccatcgg taccatgcag attaaagatt ttctcggtct gcaaatggcc
    25921 catgtcccgg aacattatct acaaaaagtc ggcgcattat ttatggcgct gccgaccatt
    25981 aatgtgggtg atgctgccat tggcattgtg acgctaggta ttcttgtttt ttggccgcgt
    26041 ctgggcattc gtttacccgg tcaccttccg gccttgctgg ctggttgcgc ggtgatgggg
    26101 attgttaacc tgctcggcgg acatgttgct accatcggtt cgcaattcca ctacgtcctg
    26161 gccgatggtt ctcagggtaa cggtattccg caactgctgc cgcaactggt gctgccgtgg
    26221 gatctgccta attcagaatt cacgctaacc tgggattcta ttcgcacact gctgcctgcg
    26281 gcattctcaa tggcaatgct cggcgcaatc gaatctctgc tctgcgccgt ggtgctggat
    26341 ggtatgaccg ggacgaaaca caaggcgaac agcgaactgg ttggacaggg actggggaat
    26401 attatcgctc cgttctttgg tggtattacc gctacagctg ccatcgcgcg ttctgccgct
    26461 aacgtccgtg ccggggcaac gtcccctatc tcggcggtga tccactctat tctggttatt
    26521 cttgccctgc tggtactggc accgctgctc tcctggctgc cgctttccgc catggcagcc
    26581 ctgctgttga tggtggcgtg gaacatgagt gaagcgcaca aagtggtcga cttgctgcgt
    26641 catgcgccga aagatgacat catcgtcatg ctgctgtgca tgtcgctgac cgtgttgttt
    26701 gatatggtta ttgccatcag cgtggggatc gtgctggcat cgctgctgtt tatgcgtcgt
    26761 atcgcacgta tgactcgcct ggcaccggta gtcgtagatg ttccagacga tgtcctggtt
    26821 ctgcgcgtta ttggcccgct gttttttgct gctgctgaag gcttattcac ggacctggag
    26881 tcacgtcttg aaggcaaacg gattgtgatt ctgaagtggg atgccgttcc ggtacttgat
    26941 gctggtggtc ttgatgcgtt ccagcgtttt gtgaagcgtc tgcccgaggg atgtgaactg
    27001 cgcgtgtgca acgtggaatt ccagccactg cgcactatgg ctcgcgctgg cattcaaccg
    27061 atcccgggac gcctggcgtt cttcccgaat cgtcgcgcgg cgatggcgga tttataagta
    27121 cgagattgac cagtcagcgc aaactgactg gtcagcaaac tgcattattt gttagctatg
    27181 acggcgatta tcgctacggc ggcaacgttt gtcatagtgg cgaacccacc agtaacggtc
    27241 gcacacctgt tcatgcccgc caacacgggc acccgccagc cagagaatgg caccgacgaa
    27301 aatacttagc actgcaccat gtgcgaaata ctggggcata ttaaactgtg gtaactggtt
    27361 gaggattgaa taccccacgc cgaccaccat taccaccaga cccaacccca tgagcacgtt
    27421 accgagtaac gaagcgtttt tgcgtttcat atgtcacctc cggaactttc tgggttgtaa
    27481 cagggaatac ccctcttcct tatgtgtaaa gtatagacaa cacccagtgg gttatgtgcg
    27541 ggcgtgatca caattacaac ccttatttca acaaaacttt acaaataaac gcctgaacac
    27601 cttgactttc taatagtcca ctcaggcaat tattacgtta tctgcatttg atacgcagtt
    27661 ttttgtcaag cggggccgca accagtaaac tacgcgccag ttatgtacac actcaggaca
    27721 aaaaacgtga cgattaaatt gattgtcggc ctggcgaacc ccggtgctga atacgccgca
    27781 acgcgacata atgctggtgc ctggttcgtt gacttactgg cagagcgttt gcgcgctccg
    27841 ctgcgcgaag aggctaaatt ctttggttat acttcgcgag tcactcttgg aggcgaagat
    27901 gtccgcctgt tagtcccgac tacatttatg aatctcagcg gcaaagccgt tgcggcgatg
    27961 gccagttttt tccgcattaa tccggacgaa attctggtgg cccacgacga actggatctg
    28021 cctcctggcg tcgccaaatt taaattgggc ggtggccatg gtggtcacaa tggactgaaa
    28081 gacatcatca gtaaattggg taataaccct aactttcacc gtttacgcat cggaatcggt
    28141 catccgggcg ataaaaataa agttgtcggt tttgtgttag gcaaaccgcc tgttagtgaa
    28201 cagaagttaa ttgatgaagc cattgacgaa gcggcgcgtt gtactgaaat gtggtttaca
    28261 gatggcttga ccaaagcaac gaaccgattg cacgccttta aagcgcaata agtcgttgtc
    28321 tgcggcattt ttgccgagtg ccgtgtataa taggcaaagt tatttccatt tctgcaatct
    28381 gttagcaata acaggttgat tattaagata ttaaggtgat ttaaatcatg ggattcaaat
    28441 gcggtatcgt cggtttgccc aacgtcggga aatctaccct gttcaacgcg ctgaccaaag
    28501 ccggtattga agcggccaac tttccattct gcaccattga gccgaacaca ggcgtcgtac
    28561 caatgcctga tcctcgcctg gatcaactgg ctgaaatcgt aaaaccgcag cgtacgcttc
    28621 ccacgaccat ggaatttgtc gatatcgccg gtctggtaaa aggcgcatcg aaaggcgaag
    28681 gtctgggtaa ccagttcctg accaacatcc gtgaaaccga agcgatcggt cacgttgttc
    28741 gctgctttga aaatgacaac atcattcacg tttccggcaa agttaacccg gctgacgata
    28801 ttgaagttat caacaccgaa ctggcgctgg cagacctcga cacctgcgaa cgtgcgattc
    28861 atcgcgtaca gaagaaagcc aaaggtggcg ataaagacgc gaaagctgag ctggcggtcc
    28921 tggaaaaatg cctgccccag ttggaaaatg caggtatgct gcgcgcgctg gatttaagcg
    28981 ctgaagagaa agcggctatt cgttacctga gcttcctgac gctgaaacca acaatgtaca
    29041 tcgccaacgt caacgaagac ggttttgaaa acaacccata tcttgaccag gtgcgtgaaa
    29101 tcgcggcgaa agaaggttct gttgtggttc cggtttgtgc tgctgttgaa gcagacattg
    29161 ccgaactgga cgacgaagaa cgtgacgagt ttatgcagga gcttgggctg gaagagccgg
    29221 gcctgaaccg tgtgatccgt gccggttata aactgctgaa cctgcaaact tacttcaccg
    29281 ctggggtgaa agaagtgcgt gcatggacca ttccggttgg agcaaccgcg ccgcaggcag
    29341 cgggcaaaat ccatactgat tttgaaaaag gctttatccg tgcacaaacc atctcgtttg
    29401 aagatttcat cacttacaaa ggtgaacaag gcgcgaaaga agcaggcaaa atgcgtgcag
    29461 aaggtaaaga ttacatcgtg aaagatggcg atgtgatgaa cttccttttc aacgtctaat
    29521 taatttattg tctcatgagg tttcgctaaa tctcatgaag agcgcaaaag tatataaaat
    29581 ccacgcaatt gcgtggattt ttcatttcac cgagtcctaa tcattcttgt aacatcctac
    29641 acttttgtgc ttattgccta tagtgaacca cgagaataat taaagacata agtgttcaga
    29701 tgacggatat ctcattagca gtgatataac gtaagttttt gtatcactac acatcagccc
    29761 ccttcacagc tgatcaaaat tgatcacgct ccaatatgct ggcaagatga aaatgataac
    29821 cagtagcaca ataacaacga taggccagaa aacccattga ccaaaaatga atatcgatgt
    29881 tttttgcttt ttcttattag taatgcaaaa atggcgcata ttgtttttgt tatgcagata
    29941 catcaagcga aattcccata actaattagg attattacaa aaaatcttca ccaaattttt
    30001 taaccgctgt ttgttgtaat atttttgcca tatttatagt aacgaattgg aataagtaac
    30061 gacaacttag ggaataactc gatataaact cacctttgta agggggatgt taaatatata
    30121 ttaattcctg tgttcctgat tttgcccggt aatagagaat tattctctga gaagctcatc
    30181 atgattgcac catacaaata tcatgatgat aagaggatat tattcattga tttaaacaaa
    30241 atgacaaaat ccatagaccc atagtctggg tactatacag gagtggtatg ggcatcaaac
    30301 aacacaatgg gaataccaaa gcagatcgtc tcgctgaatt aaaaatccgt tcgccctcaa
    30361 ttcaactgat aaaatttggc gctattggtt tgaatgcaat tatcttttcc cccctgctga
    30421 tagctgctga tacaggaagt caatatggca ccaatattac tattaatgat ggtgacagaa
    30481 ttacaggaga taccgccgat ccatcaggaa acctctatgg tgtaatgacc ccagcaggaa
    30541 acacgcctgg caatatcaac ctgggtaatg atgtcaccgt caatgtcaac gacgcctctg
    30601 gatatgcaaa aggaatcatt attcagggca aaaacagctc cctgacagct aaccgactca
    30661 cagtagatgt tgttggtcaa acctctgcca tcggcattaa cttaattggt gactataccc
    30721 atgctgactt aggcacaggc agcaccatta agagtaacga tgacggcatc attattgggc
    30781 atagctcaac actaacagcc actcaattca ccattgaaaa ctcgaacggt ataggcctaa
    30841 ccatcaatga ctatggcacc agtgtcgatc ttggaagcgg aagtaaaatc acgaccgatg
    30901 gaagtacagg tgtttatatc ggtggtctca acggcaataa cgccaatggt gctgcgcgtt
    30961 ttacggctac agacctgaca atcgatgttc agggctacag cgccatgggg ataaacgtac
    31021 agaaaaactc tgttgtcgat ctcggaacaa acagtaccat taaaaccaat ggcgataatg
    31081 ctcacggcct ctggagcttt ggccaggtta gcgcgaatgc actcactgtt gatgtaactg
    31141 gagccgcggc caatggcgtc gaagttcgtg gtggtacaac cactatcggt gcagatagcc
    31201 atatttcttc cgcgcagggc ggtggcctcg tcaccagtgg ttcagacgcg ataatcaatt
    31261 ttactggcac ggcagcgcaa cgaaacagca tcttttccgg cggttcttat ggtgcctcgg
    31321 cccagacggc aacggctgtt gtcaacatgc aaaataccga tattacagtt gatcgtaatg
    31381 gcagtctggc gctgggtttg tgggctctca gcggcggtag aataaccgga gacagtttgg
    31441 ctatcaccgg cgcggcagga gccaggggaa tttatgccat gaccaacagc cagatcgacc
    31501 tcacgagcga tctggtcatt gatatgagta cacccgacca gatggccatc gcaacgcaac
    31561 atgacgatgg ttatgccgcc agccgcatca acgcctcggg tcgtatgctt atcaacggta
    31621 gcgttctttc caaaggtggg ctaatcaatc tggatatgca ccctgggtcg gtttggacag
    31681 gttcctccct cagcgataat gtcaatggcg gaaaactgga cgttgcaatg aataacagcg
    31741 tctggaacgt aacaagtaat tctaatctcg acacgctggc gctgagccat tcaactgtcg
    31801 attttgccag ccacgggtca actgccggca catttgccac attaaacgta gagaacctga
    31861 gcggtaacag tacctttatt atgcgtgctg atgttgttgg cgagggtaat ggcgttaata
    31921 ataaagggga tttattgaat atcagcggga gtagtgctgg taatcacgta ttggctatcc
    31981 gcaaccaggg cagcgaggcc acaacgggaa atgaagttct gacagtggta aaaaccactg
    32041 acggcgcggc ctcgttcagc gcgtcttctc aggttgagtt ggggggatat ctgtacgatg
    32101 tgcgtaaaaa tggcactaac tgggagcttt acgcttccgg gacagttccg gaaccgactc
    32161 ctaatcctga acccacacca gctcccgctc agcctcccat agtcaacccc gatcctacgc
    32221 ctgaacccgc tcccacgcct aaacccacca cgaccgcaga tgctggcggc aattatctca
    32281 atgtcggtta cttattgaac tatgttgaaa accgtacgct gatgcaacgg atgggtgacc
    32341 tgcgaaatca gagtaaagac ggtaatatct ggttgcgcag ttatggggga agcctggact
    32401 cctttgccag tggcaaactg agcggctttg acatgggtta cagcggtatc cagtttggtg
    32461 gggataaacg tctctctgat gtaatgccgt tgtatgtcgg tctgtatatt ggctcaacac
    32521 atgcatcgcc ggactatagc ggaggcgacg gtaccgcacg ttcagactac atgggaatgt
    32581 acgccagtta catggcacaa aacggttttt acagcgatct cgttataaaa gcatcgcgcc
    32641 agaaaaatag tttccacgta ctggacagtc agaacaacgg cgttaacgcc aacggcactg
    32701 cgaatggaat gagcatctcc ctggaagccg ggcagaggtt caacctgtcc cctactggtt
    32761 atgggttcta tatagagccg caaacccagc ttacatacag ccaccagaat gagatgacta
    32821 tgaaggcgag taatggcctc aatatacatc tgaatcacta cgaatcgctg ctggggcgtg
    32881 ccagcatgat actggggtat gacatcaccg caggcaacag ccagctgaat gtctatgtga
    32941 agactggcgc tatccgcgag ttttcagggg ataccgaata tctgttgaac aactcccggg
    33001 agaagtacag tttcaaaggt aatggctgga ataacggcgt gggagtcagt gcacagtata
    33061 acaaacagca cacattctat ctcgaagcgg attacacgca gggtaacctc tttgatcaga
    33121 agcaagtcaa cggaggatat cgcttcagct tttaagtgcg ggatagacaa gcccttaaac
    33181 gatgctgaaa attgttagcg tgcgccggaa tgaaatacag atcactctga tgtatcagaa
    33241 atcgtttctg tctttcatac ccggcgctta aactgccctg catcaatgcc atgttgcttc
    33301 attttccgcc acagcgtagt gcggccgatc ccaagtaaag ccgacatttc ctgaatgcga
    33361 ccgcctgtga cctgggctgc gttaataatt gcctcttttt caacttccgc aaatgacaga
    33421 ctggtggaaa ggcgggtggc gctgacatca tctgtcgcct gctcggtaaa cagatgttcc
    33481 ggcaaatcac tgacgcgaat gcgcccgtta tcactactca gagcaagatt ctcgatgacg
    33541 ctgtaaagtt caaaatcgtt gcctggccat gcacaagaaa ccaggcgagc gagggcatcg
    33601 tcatcaattt tcagccgcgt agagaagcgt ttttcaagac tgcgtaattt gttattcacc
    33661 agcgccggaa tgctgccacg ccgcatacgc agaggcggga tggtaatttc aaatgcatgc
    33721 agcgcgtaat acagctggcg actaaaacga ttttgttcca ccagcattgc gaggtccgcg
    33781 gtcgttgttg caatcacttt gacatcaatt ggtattaaac gccgcgcatc cagtcgcgtg
    33841 ataaccccct gcttgataac ctgaagcaaa gcagactgta actccaccgc cagatattca
    33901 atcttttcaa gaaacagcgt gccgccgtgt gccagttcca gccgactcag acggccattt
    33961 tcattgtccg tgcgatcgcc accaataaat tcttccgcca gcgcagcatc accatataac
    34021 tcacaattga cggcgatata aggacctgca gcacgctcgc tttcattatg aattgcctga
    34081 cttagcagtg ccttgcccac gccctcttct ccacaaagca ggacaggaaa gctactgcgc
    34141 gccgcctggc gaccaaaatg aatcaagcgg cgggtttgcg gatcgtcctg tggcatatga
    34201 gcgaaggtat ggctgacttt tcctaattga ctggtcatca actgccgcat ctgttccaca
    34261 ggatggagca acaaaataaa gctggttccc tgcgtttcta ttatcggttt aagggttatc
    34321 accgcatcaa taaactggtg ctggctttca aaggttgctt ctacgtgttt gagcggatgt
    34381 gcctgtttta ttgcttgttg caatacggcg ggtaacgtta agagttcagt gattgcccgt
    34441 ccctgacttg ccgtcgcgtc aaggcgcaag acccgcgccg cctgggcatt aataaattgc
    34501 aaattaccct gctcgtccca gctaatcacg ccatcatcca tactttctaa cagggcatta
    34561 agttgattta aatgacggtt agtttcagcg agcaaactgt ccgtcagcag taaatttccg
    34621 acctcgcggg cgattgccaa cgtcaacggc aaatcagctg cggtagtttg ctcaaccgga
    34681 cacgccagcg ctattgttcc cgtcaatcgg cccttgctgt caaacaacgg cgttgcacaa
    34741 aaggcccagt tccagagtac ctgtttgaaa tgttgatcgg ccatcgtttt cacggcctga
    34801 ccagagatag ccgctaacga tagcgcacaa gtaccaataa ttccctcggc gcaatacgtg
    34861 ccgtcattga atcccagtgc acttagctgc tgcaaggttt gcggatcgcc attacggctg
    34921 agaatgcagg cggtttcatc gaggataaac agcgcacact ctcgcggtgc catatattcc
    34981 caggcatctt ccagcgcagc ctgcccgagc gtcagcatcg ctttcttacg ccgataaata
    35041 gaagcaaatg tcacgccctg ggcctgatgt ggtacgttcc atgtctcccg tttcatcagc
    35101 ttattgcatc gctcccagga ggttgcaatt aagggagata tgccccgacc atcgttgtta
    35161 aaagcgccac tcatatcccg catcccttat gacaaaaggt gttccggctc gcaattttaa
    35221 gtattaatac acgtaaaagt ttcaatatgg aacattcatt aaagcatatg tttcactatg
    35281 aaacataatt tgggctaatt ttcttttccc ttgcctgatg cacaatggat tcatcggcag
    35341 tcagtggtcg ccgtgtcgtt gaacatcatc catgccctac cgtaattgct ggagcaaaat
    35401 aatgaaaaaa ttgatcaatg atgtgcaaga cgtactggac gaacaactgg caggactggc
    35461 gaaagcgcat ccatcgctga cactgcatca ggatccggtg tatgtcaccc gagctgatgc
    35521 ccctgttgca ggaaaagtcg ccctgctgtc gggtggcggc agcggacacg agccgatgca
    35581 ctgtggttat atcggtcagg ggatgctttc gggggcctgt ccgggcgaaa ttttcacctc
    35641 accgacgccc gataaaatct ttgaatgcgc catgcaagtt gatggcggcg aaggtgtact
    35701 gttgattatc aaaaattaca ccggcgatat tcttaacttt gaaacagcga ccgagttact
    35761 gcacgatagc ggcgtaaaag tgaccactgt ggtcattgat gacgacgttg cggtaaaaga
    35821 cagtctttat actgccgggc gacgcggcgt tgccaacacc gtattaattg aaaaactcgt
    35881 aggcgcagcg gcggagcgtg gcgactcact ggacgcctgt gcggaactgg ggcgtaagct
    35941 gaataatcaa ggccactcaa taggtatcgc tctcggtgcc tgtaccgttc ctgccgcggg
    36001 caaaccttct tttaccctgg cggataatga gatggagttt ggcgtcggca ttcatggtga
    36061 gccgggtatt gaccgccgcc ccttctcttc ccttgatcaa accgtcgatg aaatgttcga
    36121 caccctgctg gtaaatggct cataccatcg cactttgcgt ttctgggatt atcaacaagg
    36181 cagttggcag gaagaacaac aaaccaaaca accgctccag tctggcgatc gggtgattgc
    36241 gctggttaac aatcttggcg caactccgct ttctgagctg tacggcgtct ataaccgcct
    36301 gaccacacgt tgccagcaag cgggattgac tatcgaacgt aatttaattg gcgcgtactg
    36361 cacctcactg gatatgaccg gtttctcaat caccttactg aaagttgatg acgaaacgct
    36421 ggcactctgg gacgccccgg tccacacccc ggcccttaac tggggtaaat aaggagaaag
    36481 caatgtcact gagcagaact caaattgtta actggctcac tcgttgtggc gatattttca
    36541 gcaccgagag cgagtatctt accggactgg atcgcgaaat tggcgatgct gaccacgggc
    36601 taaatatgaa ccgaggcttt agcaaagtgg tggaaaaact ccctgctatc gcagataaag
    36661 atatcggttt cattctcaag aataccggta tgacgctgct ttccagcgtc ggtggtgcca
    36721 gtggtccgct gttcggtacc ttctttatcc gcgccgcaca ggcgacccag gcacggcaaa
    36781 gcctgacact ggaagagctt tatcagatgt tccgcgatgg cgcggacggc gtaatcagtc
    36841 gcgggaaagc cgaacctggc gataaaacca tgtgtgatgt gtgggtgccg gtggtggaat
    36901 cgttacgtca gtccagcgag caaaatctct ctgttccggt ggcgctcgaa gctgccagta
    36961 gcatcgccga atccgctgca caaagtacga ttacgatgca agcccgcaaa ggccgcgcca
    37021 gttatctcgg tgaacgcagt attggtcacc aggatcccgg cgcgacctcg gtgatgttta
    37081 tgatgcaaat gttggcgtta gccgcaaaag agtaaggaat tggtgatggt aaacctggtc
    37141 atagtttcac atagcagccg actgggagaa ggtgtcggtg aattagcccg tcagatgtta
    37201 atgagtgata gttgtaaaat cgccattgcc gcgggaattg acgatccaca aaatcccatt
    37261 ggtaccgatg ccgtcaaagt gatggaggcc atcgaatctg ttgctgatgc cgaccatgtg
    37321 ctggtcatga tggatatggg tagcgcatta ttgagtgctg aaactgcgct ggaattgctg
    37381 gctcccgaga tcgccgcaaa agtacgtttg tgtgctgcgc cgttggtcga aggtacactg
    37441 gcagcaacgg tcagcgcggc ctcgggggcg gatatcgaca aagttatctt tgacgccatg
    37501 catgcgctgg aagccaaacg tgaacaactg ggtttaccgt cctccgacac tgaaatctct
    37561 gacacatgtc ctgcgtacga tgaagaagcc cgttctctgg cggtggtcat aaaaaaccgt
    37621 aacggcctgc atgtacgtcc ggcctcccgg ctggtttata ccttatcgac atttaatgcc
    37681 gatatgttgc tggaaaaaaa cggcaaatgc gtcacaccag agagtattaa ccagattgcg
    37741 ttactacaag ttcgctataa cgatacgctg cgcctgattg cgaaagggcc agaagctgaa
    37801 gaggcactga tcgctttccg tcagctggct gaagataact ttggtgaaac ggaggaagtc
    37861 gctccaccta ctctgcgtcc cgttccgcct gtttcgggta aagcctttta ttatcaacca
    37921 gttttatgta cggtacaggc aaaatcaacc ctgaccgtgg aagaagaaca agatcgatta
    37981 cgccaggcta ttgacttcac gttattagat ctgatgacgt taacagcgaa agcagaagcc
    38041 agcgggcttg acgatattgc cgcaatcttt tctggtcacc atacactgtt agatgatccg
    38101 gaactgctgg cggcggcaag cgaactcctt cagcatgaac attgcacggc agaatatgcc
    38161 tggcagcaag ttcttaaaga acttagccag caataccagc aactggatga tgaatatcta
    38221 caagctcgct atattgatgt ggacgatctt ctgcatcgca ccctggtcca cctgacccaa
    38281 acgaaagaag aactcccgca gtttaactcg ccaactattc tactggcgga gaacatttat
    38341 ccttccacag tactgcaact ggatccggcg gttgtaaaag gtatctgcct tagcgccgga
    38401 agtccggtat cccacagcgc cctaatcgcc cgtgaactgg ggattggctg gatttgccag
    38461 cagggtgaga aactgtatgc gatacaacca gaagaaacgc taacgctgga cgttaaaacg
    38521 caacgtttca accgtcaggg ttaagccact gttcggatgg catcgttctg atgtcatccg
    38581 gcaatttact cagcactaat ggtttttaac tacctgttat atttttcacc aatttatgat
    38641 gatttttctt cgctaatcac gaacccaacg ccgctggctg gctaataaac agacgttatt
    38701 tgatcactct atttcgccgt tatttatccc tttaattccc ttttctaaaa tgcctgacag
    38761 ttcgcagaat gagatttcga tcatgcagct agtgcgatcc tgaactaagg ttttctgata
    38821 cttgaatacc gttttattcg gtttcgccaa aggagaatga ttgatgaaat cccccgcacc
    38881 ttctcgcccg caaaaaatgg cgttaattcc agcctgtatc tttttgtgtt tcgctgcgct
    38941 atcggtgcag gcagaagaaa caccggtaac accacagccg cctgatattt tattagggcc
    39001 gctgtttaat gatgtgcaaa acgccaaact ttttccggac caaaaaacct ttgccgatgc
    39061 cgtgccgaac agcgatccgc tgatgatcct tgctgattat cggatgcagc aaaaccagag
    39121 cggatttgat ctgcgccatt tcgttaacgt caatttcacc ctgccgaaag aaggcgagaa
    39181 atatgttccg ccagaggggc agtcactgcg cgaacatatt gacggacttt ggccggtatt
    39241 aacgcgttct accgaaaaca ccgaaaaatg ggattctctg ttaccgctgc cggaacctta
    39301 tgtcgtgccg ggcggacgct ttcgcgaggt atattactgg gacagttact tcaccatgtt
    39361 aggacttgcc gaaagcggtc actgggataa agtcgcggat atggtggcca attttgctca
    39421 tgaaatagac acttacggtc atattcccaa cggcaaccgc agttactatt taagccgctc
    39481 gcaaccgccc ttctttgccc tgatggtaga gttactggcg cagcatgaag gcgatgccgc
    39541 gttgaagcaa tacctgccgc aaatgcaaaa agaatatgct tactggatgg acggtgttga
    39601 aaacctgcaa gccggacaac aggaaaaacg cgttgtcaaa cttcaggatg gtacccttct
    39661 caaccgctac tgggacgatc gcgatacgcc acgaccagag tcatgggtgg aagatattgc
    39721 caccgccaaa agcaatccga atcgacctgc cactgaaatt taccgcgacc tgcgctctgc
    39781 cgctgcgtct ggctgggatt tcagctcgcg ctggatggac aacccgcagc agttaaatac
    39841 cttacgcacc accagcatcg taccggtcga tctgaacagc ctgatgttta aaatggaaaa
    39901 aatcctcgcc cgcgccagca aagctgccgg agataacgcg atggcaaacc agtacgaaac
    39961 gctggcaaat gcccgtcaaa aagggatcga aaaatacctg tggaacgatc aacaaggctg
    40021 gtatgccgat tacgacctga aaagtcataa agtgcgcaat cagttaaccg cggccgccct
    40081 gttcccgctg tacgtcaatg cggcagcgaa agatcgcgcc aacaaaatgg cgacggcgac
    40141 gaaaacacat ctgctgcaac ccggcggcct gaacaccacg tcggtgaaaa gtgggcaaca
    40201 atgggatgcg ccaaatggct gggcaccgtt acagtgggtc gcgacagaag gattacaaaa
    40261 ctacgggcaa aaagaggtgg cgatggacat tagctggcac ttcctgacca atgttcagca
    40321 cacctatgac cgggagaaaa agctggtgga aaaatatgat gtcagcacca ccggaacggg
    40381 gggcggcggt ggcgaatatc cattacagga tggctttggc tggaccaatg gcgtgacgct
    40441 gaaaatgctg gatttgatct gcccgaaaga gcaaccgtgt gacaatgttc cggcgacgcg
    40501 tccgaccgtt aagtcagcaa cgacgcaacc ctcaaccaaa gaggcacaac ccacacctta
    40561 accagcgctt actccgacaa cgtcattcgg cttctccgga tggcgttgtc ggcagataac
    40621 accatagcta tggttttttc tacacagacg gtttttcaag tcctggcggg aagtattgcg
    40681 caaatgtttg ccatacgtgg ttgccgaaga tagcgtcatt tctacgggta agtgtttcac
    40741 tttcatcacc cggcgattta cgccgacgtt ggctatataa agcatcaatt gctacctgaa
    40801 tattgttacg gtgttcactc atcaacgttt ttttgctttc tggagttaaa ttaataatca
    40861 cataatccag acactccaga gaatttagca accaaaaaaa gaattcagac cattcctctt
    40921 cgcgtaactg aagcattgcc gaacgggtat tttggcttaa tctgccagaa ggattgatca
    40981 attctaaatg ttcaataagc gcagccaaaa ccgcctcact atccgcccca tttttaacgc
    41041 ctgactggct actgtctaaa atatttattt tctgaatcac accaaaaaca acgaaacaat
    41101 aattaaaaaa ggattctgtt gttgtt
//
LOCUS       DBBGLQ010000039        41113 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.039, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000039 DBBGLQ010000000
VERSION     DBBGLQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.039"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(84..215)
                     /locus_tag="ACIYSB_002656"
     ncRNA           complement(84..215)
                     /ncRNA_class="other"
                     /locus_tag="ACIYSB_002656"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(262..346)
                     /locus_tag="ACIYSB_002657"
     tRNA            complement(262..346)
                     /locus_tag="ACIYSB_002657"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(310..312),aa:Tyr,seq:gta)
     gene            complement(381..512)
                     /locus_tag="ACIYSB_002658"
     ncRNA           complement(381..512)
                     /ncRNA_class="other"
                     /locus_tag="ACIYSB_002658"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(556..640)
                     /locus_tag="ACIYSB_002659"
     tRNA            complement(556..640)
                     /locus_tag="ACIYSB_002659"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(604..606),aa:Tyr,seq:gta)
     gene            complement(800..1642)
                     /gene="purU"
                     /locus_tag="ACIYSB_002660"
     CDS             complement(800..1642)
                     /gene="purU"
                     /locus_tag="ACIYSB_002660"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGC5429683.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLTRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1692..2150)
                     /gene="ychJ"
                     /locus_tag="ACIYSB_002661"
     CDS             complement(1692..2150)
                     /gene="ychJ"
                     /locus_tag="ACIYSB_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGC5429684.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            2263..3168
                     /gene="rssA"
                     /locus_tag="ACIYSB_002662"
     CDS             2263..3168
                     /gene="rssA"
                     /locus_tag="ACIYSB_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGC5429685.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3260..4273
                     /gene="rssB"
                     /locus_tag="ACIYSB_002663"
     CDS             3260..4273
                     /gene="rssB"
                     /locus_tag="ACIYSB_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGC5429686.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            4475..5383
                     /gene="galU"
                     /locus_tag="ACIYSB_002664"
     CDS             4475..5383
                     /gene="galU"
                     /locus_tag="ACIYSB_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGC5429687.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(5527..5940)
                     /gene="hns"
                     /locus_tag="ACIYSB_002665"
     CDS             complement(5527..5940)
                     /gene="hns"
                     /locus_tag="ACIYSB_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGC5429688.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            6545..7162
                     /gene="tdk"
                     /locus_tag="ACIYSB_002666"
     CDS             6545..7162
                     /gene="tdk"
                     /locus_tag="ACIYSB_002666"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGC5429689.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVDSLTAIQERHRHD"
     gene            complement(7444..8340)
                     /locus_tag="ACIYSB_002667"
                     /pseudo
     CDS             complement(7444..8340)
                     /locus_tag="ACIYSB_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(8464..11139)
                     /gene="adhE"
                     /locus_tag="ACIYSB_002668"
                     /gene_synonym="adhC"
     CDS             complement(8464..11139)
                     /gene="adhE"
                     /locus_tag="ACIYSB_002668"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGC5429690.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            11532..11585
                     /gene="ychE"
                     /locus_tag="ACIYSB_002669"
     CDS             11532..11585
                     /gene="ychE"
                     /locus_tag="ACIYSB_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGC5429691.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            11616..12263
                     /gene="ychE"
                     /locus_tag="ACIYSB_002670"
     CDS             11616..12263
                     /gene="ychE"
                     /locus_tag="ACIYSB_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGC5429692.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(12421..12717)
                     /locus_tag="ACIYSB_002671"
     CDS             complement(12421..12717)
                     /locus_tag="ACIYSB_002671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429693.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            13001..14632
                     /gene="oppA"
                     /locus_tag="ACIYSB_002672"
     CDS             13001..14632
                     /gene="oppA"
                     /locus_tag="ACIYSB_002672"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGC5429694.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            14718..15638
                     /gene="oppB"
                     /locus_tag="ACIYSB_002673"
     CDS             14718..15638
                     /gene="oppB"
                     /locus_tag="ACIYSB_002673"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGC5429695.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            15653..16561
                     /gene="oppC"
                     /locus_tag="ACIYSB_002674"
     CDS             15653..16561
                     /gene="oppC"
                     /locus_tag="ACIYSB_002674"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGC5429696.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            16573..17586
                     /gene="oppD"
                     /locus_tag="ACIYSB_002675"
     CDS             16573..17586
                     /gene="oppD"
                     /locus_tag="ACIYSB_002675"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGC5429697.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            17583..18587
                     /gene="oppF"
                     /locus_tag="ACIYSB_002676"
     CDS             17583..18587
                     /gene="oppF"
                     /locus_tag="ACIYSB_002676"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGC5429698.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(18640..18969)
                     /gene="yciU"
                     /locus_tag="ACIYSB_002677"
     CDS             complement(18640..18969)
                     /gene="yciU"
                     /locus_tag="ACIYSB_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGC5429699.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(19004..20464)
                     /gene="cls"
                     /locus_tag="ACIYSB_002678"
     CDS             complement(19004..20464)
                     /gene="cls"
                     /locus_tag="ACIYSB_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGC5429700.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            20607..20780
                     /gene="yciY"
                     /locus_tag="ACIYSB_002679"
     CDS             20607..20780
                     /gene="yciY"
                     /locus_tag="ACIYSB_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGC5429701.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(20835..22088)
                     /gene="kch"
                     /locus_tag="ACIYSB_002680"
     CDS             complement(20835..22088)
                     /gene="kch"
                     /locus_tag="ACIYSB_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGC5429702.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDELETKADSKESAQK"
     gene            complement(22388..22684)
                     /gene="yciI"
                     /locus_tag="ACIYSB_002681"
     CDS             complement(22388..22684)
                     /gene="yciI"
                     /locus_tag="ACIYSB_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGC5429703.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            22908..23627
                     /gene="tonB"
                     /locus_tag="ACIYSB_002682"
     CDS             22908..23627
                     /gene="tonB"
                     /locus_tag="ACIYSB_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGC5429704.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(23667..24065)
                     /gene="yciA"
                     /locus_tag="ACIYSB_002683"
     CDS             complement(23667..24065)
                     /gene="yciA"
                     /locus_tag="ACIYSB_002683"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGC5429705.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(24170..24709)
                     /gene="yciB"
                     /locus_tag="ACIYSB_002684"
     CDS             complement(24170..24709)
                     /gene="yciB"
                     /locus_tag="ACIYSB_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGC5429706.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(24739..25482)
                     /gene="yciC"
                     /locus_tag="ACIYSB_002685"
     CDS             complement(24739..25482)
                     /gene="yciC"
                     /locus_tag="ACIYSB_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGC5429707.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(25510..25599)
                     /locus_tag="ACIYSB_002686"
                     /pseudo
     CDS             complement(25510..25599)
                     /locus_tag="ACIYSB_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            25839..26477
                     /gene="ompW"
                     /locus_tag="ACIYSB_002687"
     CDS             25839..26477
                     /gene="ompW"
                     /locus_tag="ACIYSB_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGC5429708.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(26537..27043)
                     /gene="yciE"
                     /locus_tag="ACIYSB_002688"
     CDS             complement(26537..27043)
                     /gene="yciE"
                     /locus_tag="ACIYSB_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGC5429709.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQNIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(27089..27589)
                     /gene="yciF"
                     /locus_tag="ACIYSB_002689"
     CDS             complement(27089..27589)
                     /gene="yciF"
                     /locus_tag="ACIYSB_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGC5429710.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLAINNVNK
                     KAENKA"
     gene            complement(27675..27854)
                     /gene="yciG"
                     /locus_tag="ACIYSB_002690"
     CDS             complement(27675..27854)
                     /gene="yciG"
                     /locus_tag="ACIYSB_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGC5429711.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(28235..29041)
                     /gene="trpA"
                     /locus_tag="ACIYSB_002691"
     CDS             complement(28235..29041)
                     /gene="trpA"
                     /locus_tag="ACIYSB_002691"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGC5429712.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(29041..30234)
                     /gene="trpB"
                     /locus_tag="ACIYSB_002692"
     CDS             complement(29041..30234)
                     /gene="trpB"
                     /locus_tag="ACIYSB_002692"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGC5429713.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(30246..31607)
                     /gene="trpCF"
                     /locus_tag="ACIYSB_002693"
     CDS             complement(30246..31607)
                     /gene="trpCF"
                     /locus_tag="ACIYSB_002693"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415778.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGC5429714.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFNFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEEQERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGTDNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(31608..33203)
                     /gene="trpD"
                     /locus_tag="ACIYSB_002694"
     CDS             complement(31608..33203)
                     /gene="trpD"
                     /locus_tag="ACIYSB_002694"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGC5429715.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(33203..34765)
                     /gene="trpE"
                     /locus_tag="ACIYSB_002695"
     CDS             complement(33203..34765)
                     /gene="trpE"
                     /locus_tag="ACIYSB_002695"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGC5429716.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(34787..34880)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(34857..34901)
                     /gene="trpL"
                     /locus_tag="ACIYSB_002696"
     CDS             complement(34857..34901)
                     /gene="trpL"
                     /locus_tag="ACIYSB_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGC5429717.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            35039..35920
                     /gene="rnm"
                     /locus_tag="ACIYSB_002697"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             35039..35920
                     /gene="rnm"
                     /locus_tag="ACIYSB_002697"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGC5429718.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGVVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            35917..36537
                     /gene="yciO"
                     /locus_tag="ACIYSB_002698"
     CDS             35917..36537
                     /gene="yciO"
                     /locus_tag="ACIYSB_002698"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGC5429719.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            36565..38460
                     /gene="yciQ"
                     /locus_tag="ACIYSB_002699"
     CDS             36565..38460
                     /gene="yciQ"
                     /locus_tag="ACIYSB_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGC5429720.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLQFSNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNASAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQILIRV
                     NEGATRNNAVLLSAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRSDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFTAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            38671..39546
                     /gene="rluB"
                     /locus_tag="ACIYSB_002700"
     CDS             38671..39546
                     /gene="rluB"
                     /locus_tag="ACIYSB_002700"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGC5429721.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(39586..40176)
                     /gene="cobO"
                     /locus_tag="ACIYSB_002701"
     CDS             complement(39586..40176)
                     /gene="cobO"
                     /locus_tag="ACIYSB_002701"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGC5429722.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(40173..40931)
                     /gene="yciK"
                     /locus_tag="ACIYSB_002702"
     CDS             complement(40173..40931)
                     /gene="yciK"
                     /locus_tag="ACIYSB_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGC5429723.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
BASE COUNT        10364 a         9793 c        10318 g        10638 t
ORIGIN      
        1 agtcgaagct tctcatcctt ccccgcatgg gcagaatatt tgattgcgga ttcgcttgag
       61 agttcaggga cttttgaaag tgatggtggt gggggaagga ttactcagcg ctgcgcgctt
      121 cgcccttcgg gtcgttgcct gcggcaacgc tctctcgctg gcgctcgagt cgaaccttgg
      181 tcgaagcttc tcatccttcc ccgcatgggc agaatattta attgcggatt cgttgggaag
      241 ttcagggact tttgaaagtg atggtggtgg gggaaggatt cgaaccttcg aagtcgatga
      301 cggcagattt acagtctgct ccctttggcc gctcgggaac cccaccaggg gtaattcaaa
      361 ttttgaggta atgcttgaga tggtggtggg ggaaggatta ttcgtcgcct cgctcctcac
      421 ccttcgggcc gttgcctgtg gcaacgttct ctcgctttcg ctcgaatcga accttagtcg
      481 aaggttctca cccttccccg atgagtgcaa actttcacaa tctcaccgaa gttaccacat
      541 cgctgtggtg aattatggtg gtgggggaag gattcgaacc ttcgaagtcg atgacggcag
      601 atttacagtc tgctcccttt ggccgctcgg gaaccccacc acggggtaat gctttttact
      661 ggcctgctcc cttatcggga agcggggcgc atcatatcaa atgacgcgcc gctgtaaagt
      721 gttacgttga gaaaaatgaa ctggttgcgt aattttcatc cgtaacggat taaaggtaac
      781 cagttatttt tgctggcgat taaagaataa tcgttcgatt accgtaaaca aagacgcgct
      841 gtgccagtac tttgtatagt gcacgactta agacgttttt ctcgacgtca cgacctgcgc
      901 gcatcatatc ttcagctgtg taggtatgat cgacatgaat aacgtcctgc atgatgattg
      961 ggccttcgtc cagattgtca ttcacatagt gagcggttgc gccaataatc ttcacaccac
     1021 gttcataggc ctggtgataa ggacgtgcgc caataaacgc tggcaggaat gaatggtgaa
     1081 tattgatgat cttattcggg aagcgtgcca caaattccgg cgttaatacc cgcatatact
     1141 tcgccagcac cacgtagtca ggttgataag catcaatggc atccgccatc ttttgatcgt
     1201 gctcgttgcg ggttaaccct tcatggctta ccagctcaaa cggaatatca aaacgctcaa
     1261 ccagagaacg taaagtatcg tggttaccaa taactgccgc gatttcgaca tccaggccgc
     1321 cgtaattggc tttcatcaac aaatcgccaa ggcaatgcgc ttctttagtg accagaatca
     1381 ctatccggcg acgaccggca ggattcagct cacgcacgga gccttctggc aatgcgctat
     1441 cgagatccgc cagcagggtg gaatcattaa aaatcccttc cagttccgtg cgcataaaaa
     1501 agcgcccggt acggtgatca acaaattcat tgttctgtac gatatttaac tcgtgcttgt
     1561 agcaaatatt ggtaatacgt gcgatcagac ctttttggtc cggacaaata gtacgcagaa
     1621 ctttacgttg gagtgaatgc attgctggaa aaaccttgtt gagagtgttt gctaaaccgt
     1681 accgtcaacc attattggcc gcagcacttt ttaaattttt tacctgaacc acaagggcag
     1741 ggatcgttgc gaccaaactg cggacgtgta ccgtcaatat agtaccattg accgttttct
     1801 ttcaaaaatc gcgaacgttc aattattgca cctgttttac caccttcggt aaatcgagca
     1861 acaaaactga caaatccgat attgtcggcg tcctgccaac aatgttcaaa tacggtcaat
     1921 cccaaccatt cagtatgcgc aaatccggcc atcaattcgg cacgtaacgc cgctgctcca
     1981 caagagggat gccaggtctt tattaaataa tctgcgtctt gcatcacaaa agcgcagtaa
     2041 cgcgaacgca tgagatgttc tggatcaggt gcaacctttt caccagacac ataagggtgg
     2101 caacataggc tatactcgac agcactacca cagggacaaa gctgagacac aaataatctc
     2161 cctggaaaca ataacggcgt attaaccgcc tgagtagcac tatgttaacc gagcagtagc
     2221 gatgtggcta cgattgcatt ccaggggaat cttgcgggaa taatgagaaa gataaaaata
     2281 gggctggcgc tgggatctgg cgcggcgaga ggttggtcgc atattggcgt tattaatgcg
     2341 ctaaaaaaag tgggtattga aattgatatc gttgcaggat gttcaattgg ttcgctggtg
     2401 ggcgctgcct atgcatgcga tcgattatct gcgctggaag attgggtgac ctctttcagt
     2461 tattgggatg ttttacgcct gatggatctc tcctggcagc gcggtgggtt actgcgcggc
     2521 gagcgtgtct tcaatcaata ccgcgaaata atgccggaaa cagagatcga aaattgttcc
     2581 cgtcgctttg cggctgttgc caccaattta agtacgggac gtgaattatg gtttactgaa
     2641 ggcgatctcc atcttgctat tcgcgcatca tgcagtattc caggactcat ggcacctgtt
     2701 gcacataacg gctactggct ggttgatgga gcagtcgtta acccaattcc tatttccctc
     2761 acgcgtgcat tgggggctga tattgtgata gcggttgacc tgcagcacga tgctcatttg
     2821 atgcaacaag atttgctctc ctttaatgtc agtgaagaaa atagcgagaa tggtgattct
     2881 ctgccgtggc atgcgcgtct gaaagaaagg ttaggcagca taacgacacg tcgggcggtg
     2941 acagcgccaa cggcaacaga gattatgacc acttctatcc aggtgctgga gaaccgcctt
     3001 aaaaggaacc gcatggcagg tgatccgccc gatattctga ttcaacctgt ttgcccgcaa
     3061 atatctacgc ttgatttcca tcgcgcgcac gctgccattg cggccggaca gctggcagtg
     3121 gaaaggaaaa tggacgaact tttgccgttg gtacgcacca acatttgacc agaattttta
     3181 tctacactta agttaattct gacaggcgca ggtggcaata gcatgccact attgagtaaa
     3241 gccagtcagg ggagagaaca tgacgcagcc attggtcgga aaacagattc tcattgttga
     3301 agatgagcag gtatttcgct cgcttctgga ttcatggttt tcctcattgg gagcgacaac
     3361 ggtactggcg gctgatgggg tggatgccct tgagttgctg ggaggtttca ctccagacct
     3421 gatgatatgt gatatcgcga tgccacgaat gaacgggctt aaactgctgg agcatatacg
     3481 taacagaggc gaccagaccc cagttctggt gatatctgcc actgaaaata tggcagatat
     3541 tgccaaagcg ttacgtctgg gcgttgaaga tgttttgctg aaaccagtta aagatctgaa
     3601 tcgcttgcgc gagatggttt ttgcctgtct ctatcccagc atgtttaatt cgcgcgttga
     3661 ggaagaggaa aggctttttc gcgactggga tgcaatggtt gataaccctg ccgcagcggc
     3721 gaaattatta caggaactac aaccgccggt tcagcaggtg atttcccatt gccgggttaa
     3781 ttatcgtcaa ttggttgccg cggacaaacc cggcctggtg cttgatattg ccgcactttc
     3841 ggaaaacgat ctggcatttt attgccttga tgtcacccga gctggacata atggcgtact
     3901 tgctgccttg ttattacgcg cattgtttaa cggattatta caggaacagc ttgcacacca
     3961 aaatcaacgg ttgccagagt tgggcgcgtt attgaagcag gtaaaccatt tacttcgtca
     4021 ggccaatctg ccggggcagt ttccgctatt agttggctat tatcatcgcg aactgaaaaa
     4081 tctcattctg gtttctgcgg gtctgaatgc gacgttaaat accggcgaac accaggtgca
     4141 aatcagtaat ggtgttccgt taggcacttt aggtaacgct tatttgaatc aattgagcca
     4201 gcgatgcgat gcctggcaat gccaaatatg gggaaccggt ggtcgactgc gcttgatgtt
     4261 gtctgcagaa tgagcaaacg ataacgcggg ctaaatttgc attacctgct aatgtcggct
     4321 ggtggtacta tcgtcgccat tcgtataagt aattgtctta attatgctaa ctcgcctcct
     4381 tttcagaact tagccccttc ggggtgctga tatactggga tgcgatacag aaatatgaac
     4441 acgttcaaaa cacgaacagt ccaggagaat ttaaatggct gccattaata cgaaagtcaa
     4501 aaaagccgtt atccccgttg cgggattagg aaccaggatg ttgccggcga cgaaagccat
     4561 cccgaaagag atgctgccac ttgtcgataa gccattaatt caatacgtcg tgaatgaatg
     4621 tattgcggct ggcattactg aaattgtgct ggttacacac tcatctaaaa actctattga
     4681 aaaccacttt gataccagtt ttgaactgga agcaatgctg gaaaaacgtg taaaacgtca
     4741 actgcttgat gaagtgcagt ctatttgtcc accgcacgtg actattatgc aagttcgtca
     4801 gggtctggcg aaaggcctgg gacacgcggt attgtgtgct cacccggtag tgggtgatga
     4861 accggtagct gttattttgc ctgatgttat tctggatgaa tatgaatccg atttgtcaca
     4921 ggataacctg gcagagatga tccgccgctt tgatgaaacg ggtcatagcc agatcatggt
     4981 tgaaccggtt gctgatgtga ccgcatatgg cgttgtggat tgcaaaggcg ttgaattagc
     5041 gccgggtgaa agcgtaccga tggttggtgt ggtagaaaaa ccgaaagcgg atgttgcgcc
     5101 gtctaatctc gctattgtgg gtcgttacgt acttagcgcg gatatttggc cgttgctggc
     5161 aaaaacccct ccgggagctg gtgatgaaat tcagctcacc gacgcaattg atatgctgat
     5221 cgaaaaagaa acggtggaag cctatcatat gaaagggaag agccatgact gcggtaataa
     5281 attaggttac atgcaggcct tcgttgaata cggtattcgt cataacaccc ttggcacgga
     5341 atttaaagcc tggcttgaag aagagatggg cattaagaag taacatccgt atcggtgtta
     5401 tccacgaaac ggcgttgagc aatcgacgcc gtttttttat agcttattct tattaaattg
     5461 tcttaaaccg gacaataaaa aatcccgccg ctggcgggat tttaagcaag tgcaatctac
     5521 aaaagattat tgcttgatca ggaaatcgtc gagggattta ccttgctcat ccattgcttt
     5581 tttgattaca gctggagtac ggccttggcc agtccaggtt ttagtttcgc cgttttcgtc
     5641 aacgtagcta tattttgccg gacgctgagc acgtttagct ttggtgccag atttaacggc
     5701 agcaaggcta ttcagcagtt cgttcgggtc aataccgtca gcgatcagca tttcgcgata
     5761 ttgctgcagt ttacgagtgc gctcttcaac ttcagcagca gccgcgcttt cttcttcgcg
     5821 acgttcgtta acaacaactt ctaatttttc cagcatttct tccagcgttt caagtgtaca
     5881 ttctcttgcc tgcgcacgaa gagtacggat gttgttcaga attttaagtg cttcgctcat
     5941 tgtagtaatc tcaaacttat attggggtgg tttgttgagg taataataga gccttaaatt
     6001 cagttgtgca atagccagga atgtaaggaa ttcaaaattg ttctttattt tgtgccgcca
     6061 ataaatatct tttcataaaa ttagccagaa aagacgcggc atatagccct atttacaccg
     6121 atgatttcgc agcacgtgag gttaaaactt cctgattcat gtcacatttt atggggagat
     6181 tatcgtaggc tgacgacctt tcagtcttct gtattagttg tgtttacgag aattccctat
     6241 taagcgaatg atgaaaagta gaacagtcgc aataagagca tggacttagt attgcactat
     6301 ctcctggagg tcaacagagg gctattactt gcgcaacagg ttaaagattg tgaatagtta
     6361 ccagcagtca tttacccgct tataacaagc gaggcagttg taatgatagc tcagaaggat
     6421 tatgcaaggc ttcgtaaggg agaacgcata tacccacttc tgtgcatact gttgagctga
     6481 aaaactgacg aattatgata aactccagcc aactttattt catatcattg agggcctgtg
     6541 gctgatggca cagctatatt tctactattc cgcaatgaat gcgggtaagt ctacagcatt
     6601 gttgcaatct tcatacaatt accaggaacg cggcatgcgc actgtcgtat atacggcaga
     6661 aattgatgat cgctttggtg ccgggaaagt cagttcgcgt ataggtttgt catcgcctgc
     6721 aaaattattt aaccaaaatt catcattatt tgatgagatt cgtgcggaac atgaacagca
     6781 ggcaattcat tgcgtactgg ttgatgaatg ccagttttta accagacaac aagtatatga
     6841 attatcggag gttgtcgatc aactcgatat acccgtactt tgttatggtt tacgtaccga
     6901 ttttcgaggt gaattattta ttggcagcca atacttactg gcatggtccg acaaactggt
     6961 tgaattaaaa accatctgtt tttgtggccg taaagcaagc atggtgctgc gtcttgatca
     7021 agcaggcaga ccttataacg aaggtgagca ggtggtaatt ggtggtaatg aacgatacgt
     7081 ttctgtatgc cgtaaacact ataaagaggc gttacaagtc gactcattaa cggctattca
     7141 ggaaaggcat cgccacgatt aataagaatt tctttactga cagggtgagc agggcacttt
     7201 tatcctgtca gttcgtttta cgcacttctt ccgggctata tacccttctc ggcagttttt
     7261 taacgccgct atacgcctca cagggctctt aagcaccgac gttgacttgt gacctgtaaa
     7321 gtacaatatc cctgtgttta ggcgttatac atcgtcgcaa atatgatgaa ggctaatgct
     7381 gtcggtttat ggaaaagttg ctttgggtaa acaaaaaata cggccccaga agggcaatgc
     7441 cgttcactta agaggagcgg cactatgttt cacaggataa cgggtttttg atatcttaac
     7501 cgacctcggc cttgatggtc gggggcgttt tgttatgaac accacttcca gagcaccccg
     7561 aagatgctcc agtcgtttcg ggatggtccc tggtgacgcc gtgtttccca gctctatcat
     7621 ttctgatgcg atattcttcc acgcaggcag tagccagtgg cggttacaac ccttctggtt
     7681 cagcgtcagc agcaggtctt cgctgtaaaa aagtttatca aacaacgtaa tagagttatc
     7741 cgggatggtg gcgagcatgg agtgggccag cacagtttcg ctctgccggt aaggtgcggt
     7801 cacggcattc agcagaatgt gacttcccag gttcattaag gccaccagac gcataaccgg
     7861 gtaggcgttc tgccgcttag tggatgtgtt ggcagaccca taatattcac gcagctcggg
     7921 tttatcaggt gtcctgaact gtgcgccatc aatggcaaaa agttgcaggc cgtgccagtc
     7981 atccttcagg taacgttccg cgcccctgtc tgtgcggtct ggcggaagag ccattccact
     8041 ggggcggccc ccacgcgctg acgcgcctgg gtgacagcgc tgcgggccag caggttcatc
     8101 cccgcttcgc catccgcgct caggttcaga cggcgaacaa catcggtaat tggctcattg
     8161 caccaccatc cagataacca tgtcccccgg taaacgacgg cggcgaacgg tcgcatgagc
     8221 agaaagcgtc aggcagtgtt gtatccactc ggtgggaagg tgttctgcaa atagttgtgc
     8281 agagggcgga ggcataagcg gatggtcact gaaatcgagc agatcattga gaagtggcat
     8341 aagaaaacgg ctccctgttg tggaagccgt tatagtgcct cagtttaagg atcggtcaac
     8401 taatccttaa ctgatcggca ttgcccagaa ggggccgttt atgttgccag acagcgctac
     8461 tgattaagcg gattttttcg cttttttctc agctttagcc ggagcagctt ctttcttcgc
     8521 tgcagtttca ccttctacat aatcacgacc gtagtaggta tccagcagaa tctgtttcag
     8581 ctcggagatc agcgggtaac gcgggttagc gccggtgcac tggtcatcga atgcatcttc
     8641 agacagttta tccacgttcg ccaggaagtc tgcttcctga acgccagctt cacggataga
     8701 tttcggaata cccagttcag ctttcagcgt ttccagccat gccagcagtt tctcgatctt
     8761 agcagcagta cggtcccccg gtgcgctcag acccaagtgg tcggcaattt cagcataacg
     8821 acggcgagcc tgcggacggt catactggct gaatgcagtc tgcttggtcg ggttgtcgtt
     8881 cgcattgtag cgaataacgt tacaaatcag cagggcgttt gccagaccgt gcggaatatg
     8941 gaactgggaa cccagtttgt gcgccattga gtgacataca cccaggaagg cgttcgcaaa
     9001 cgcgataccc gcgatagtcg ctgcactgtg aacacgttca cgcgctaccg gatttttaga
     9061 cccttcgtgg taggacgctg gcagatattc tttcagcagt ttcagtgcct gcagagcctg
     9121 accatcagag aactcagatg ccagtacaga aacataagct tccatggcgt gagttactgc
     9181 gtccagacca ccgaaagcac acagggactt cggcatgtcc ataaccaggt tggcgtcgac
     9241 aatcgccata tccggagtca gcgcatagtc tgccagtgga tatttctgac cagtagcgtc
     9301 gtcagttaca accgcaaacg gagtgacttc agaacctgta ccagaagtgg tggtgacagc
     9361 gatcattttc gctttcacgc ccattttcgg gaacttgtag atacgtttac ggatatccat
     9421 aaagcgcagc gccagctctt cgaagtgagt ttccggatgt tcgtacataa cccacatgat
     9481 cttcgcggcg tccatcgggg aaccaccacc cagcgcgata atcacgtctg gtttgaagga
     9541 gtttgccagt tctgcacctt tacgaacgat gctcagggtc gggtccgctt ctacttcgaa
     9601 gaagacttca gtttcaacgc ctgctgcttt cagtacggaa gtgatctgat cagcataacc
     9661 attgttgaac aggaagcggt cagtcacgat gagcgcacgt ttgtggccat cagtaatcac
     9721 ttcatccagc gcgattggca gggagccacg gcggaagtag atagatttcg gaagtttgtg
     9781 ccacaacatg ttttcagctc gcttagcaac ggttttcttg ttgatcaggt gtttcggacc
     9841 aacgttttca gagatggagt taccacccca agaaccacaa cccagagtca gggaaggtgc
     9901 gagtttgaag ttatacaggt caccgatacc accctgagac gctggggtgt taatcaggat
     9961 acgcgccgtt ttcattttct gaccgaagta agaaacgcga gccggttggt tatcctggtc
    10021 agtgtacagg caagaggtat gaccgatacc gcccatagca accagtttct ctgctttttc
    10081 taccgcgtct tcgaaatctt tagcgcggta cattgccaga gtcggggaca gtttttcatg
    10141 tgcgaacggt tcgctttcat caacaacggt cacttcaccg atcagaatct tggtgttttc
    10201 tggtacagag aagcctgcca gttcagcaat tttataggct ggctgaccaa cgatagccgc
    10261 gttcagcgca ccgtttttca ggataacatc ctgaacagct ttcagctctt taccctgcaa
    10321 cagatagccg ccgtgggttg caaaacgttc acgtacagcg tcataaacag agtcaacaac
    10381 aacaacagac tgttcagaag cacagattac gccgttgtcg aaggttttgg acatcagtac
    10441 agatgcaact gcacgtttga tatcagcagt ttcatcgata acaactggag tgttgcccgc
    10501 gcctacaccg atagctggtt taccggagct gtatgcggct ttaaccatgc ccggaccacc
    10561 agtcgcgagg atcaggttga tgtctgggtg gtgcatcagt gcgttagaca gttcaacaga
    10621 aggttgatcg atccagccga tcagatcttt cggagcaccg gcagcgatag cagcctgcag
    10681 aacgatatca gccgctttgt tggtggcatc ttttgcacgc gggtgcgggg agaagataat
    10741 ggcgttacgg gtcttcagac tgatcagcga tttgaagata gcagttgaag tcgggttagt
    10801 ggtcggaacg ataccgcaaa taataccgat tggttcagcg atagtgatgg taccaaaagt
    10861 gtcgtcttca gacagaacac cacaggtttt ttcatcttta taggcgttgt agatatattc
    10921 agaagcaaag tggtttttga tcactttatc ttcgacgata cccatgccgg attcggcaac
    10981 ggccattttc gcgagtggga ttcgagcatc tgcagcagcc agagcggcgg cgcggaagat
    11041 tttgtctact tgctcttgag tgaaactggc atattcacgc tgggcttttt ttacacgctc
    11101 tacgagtgcg ttaagttcag cgacattagt aacagccata atgctctcct gataatgtta
    11161 aactttttta gtaaatcatc tgctcgaata cgagagtata gtcagtgcgg tgatgatttg
    11221 cttaacctat gaaaatcaaa agcttactcg cgctcacact cactgtgatt tactaaaaga
    11281 gtttaaacat tagagttatt atctctaatg cgtcacttcc aggtggcgta agcaagatta
    11341 ctcacttctg ggtactgatt acgtgatcca aatcaaattt ttgcaaagct gacacctttc
    11401 agcatcgctt ttcgccatta tagctaacag ttaataaatt gtagtatgat ttggtggcta
    11461 cattagcatg ttttgcacaa ctagataaca ataacgaatg atagcaattt taagtagtta
    11521 ggaggtgaaa aatgctgtca aaaggcgtat tgtcagcgcg tcttttcaac cttatttatg
    11581 gctaacatta tccggctttt gcttcggagc taaccgtgat tcagaccttt tttgattttc
    11641 ccgtttactt caaatttttc atcgggttat ttgcgctggt caacccggta gggattattc
    11701 ccgtctttat cagcatgacc agttatcaga cagcggcagc gcgaaacaaa actaacctta
    11761 cagccaacct gtctgtggcc attatcttgt ggatctcgct ttttctcggc gacacgattc
    11821 tacaactttt tggtatatca attgattcgt tccgtatcgc cgggggtatc ctggtggtga
    11881 caatagcgat gtcgatgatc agcggcaagc ttggcgagga taaacagaac aagcaagaaa
    11941 aatcagaaac cgcggtacgt gaaagcattg gtgtggtgcc actggcgttg ccgttgatgg
    12001 cggggccagg ggcgatcagt tctaccatcg tctggggtac gcgttatcac agcattagct
    12061 atctgtttgg tttctttgtg gctattgcat tgttcgcttt atgttgttgg ggattgttcc
    12121 gcatggcacc gtggctggta cgggttttac gccagaccgg catcaacgtg attacgcgta
    12181 ttatggggct attgctgatg gcattgggga ttgaatttat cgttactggt attaagggga
    12241 ttttccccgg cctgcttaat taattccttt caaatgaaac ggagctgcca tgctccgttt
    12301 acttcgtcat tatttttact ttgttcccgc gcagttatca aaagcaaaag gaataggtaa
    12361 aaatattctt ctcaaattac agttagttat aaggatttcc ttaactgctt ctcctcacca
    12421 tcatgttatt ttcgccacat cataatcctg ggcttgctga agaataattg aaatgatatt
    12481 agtaattcca ctgcctttgg tagaggaaag tgctaaataa taatcaattg ttaaattatt
    12541 gtgcatttca ctactggaac tgtaatcaga aaagatagac atgcttagcc aatctctatt
    12601 tgattgaatt gaaagatgtt tgttaaggca tggatgcaag ctatagattc tgatacggtc
    12661 aataaaagag aattgcttaa caattttgca aaatgtattg gcgagtaaga accgcatttg
    12721 gtactttccg ggcaaccgcc agacgattct ttattggtaa tgagaataat taacaattaa
    12781 agagcgtcgc gaaagaataa tgtgtctcga caggggagac acagtacgaa tcgacataag
    12841 gtgatcgtct gaatcaccag aataaataaa gtcggtgata gtaatacgta acgataaagt
    12901 aacctgacag cagaaagtct ccgagcctgt gcagggtccc aatccgggat tacacatgct
    12961 ggttaatacc agtaattata atgagggagt ccaaaaaaca atgaccaaca tcaccaagag
    13021 aagtttagta gcagctggcg ttctggctgc gctaatggca gggaatgtcg cgctggcagc
    13081 tgatgtaccc gcaggcgtca cactggcgga aaaacaaaca ctggtacgta acaatggttc
    13141 agaagttcag tcattagatc cgcacaaaat tgaaggtgtt ccggagtcta atatcagccg
    13201 agacctgttt gaaggcttac tggtcagcga tcttgacggt catccagcac ctggcgtcgc
    13261 tgaatcctgg gataataaag acgcgaaagt ctggaccttc catttgcgta aagatgcgaa
    13321 atggtctgat ggcacgccag tcacagcaca agactttgtg tatagctggc aacgttctgt
    13381 tgatccgaac actgcttctc cgtatgccag ttatctgcaa tatgggcata tcgccggtat
    13441 tgatgaaatt cttgaaggga aaaaaccgat taccgatctc ggcgtgaaag ctattgatga
    13501 tcacacatta gaagtcacct taagtgaacc cgttccgtac ttctataaat tacttgttca
    13561 cccatcaact tcaccggtgc caaaagccgc tatcgagaaa ttcggcgaaa aatggaccca
    13621 gcctggtaat atcgtcacca acggtgccta taccttaaaa gattgggtcg taaacgaacg
    13681 aatcgttctt gaacgcagcc cgacctactg gaacaacgcg aaaaccgtta ttaaccaggt
    13741 aacctatttg cctattgctt ctgaagttac cgatgtcaac cgctaccgta gtggtgaaat
    13801 cgacatgacg tataacaaca tgccgatcga attgttccag aagctgaaaa aagagatccc
    13861 ggacgaagtt cacgttgatc catacctgtg cacttactat tacgaaatta acaaccagaa
    13921 accgccattc aacgatgtgc gtgtgcgtac cgcactgaaa ctaggtatgg accgcgatat
    13981 cattgttaat aaagtgaaag cgcagggcga catgcccgcc tatggttaca ctccaccgta
    14041 tactgatggc gcaaaattga ctcagccaga atggtttggc tggagccagg aaaaacgtaa
    14101 cgaagaagcg aaaaaactgc tggctgaagc gggttatacc gcagacaaac cgttgaccat
    14161 caacctgttg tataacacct ccgatctgca taaaaagctg gcgattgctg cctcttcatt
    14221 gtggaagaaa aacattggtg taaacgtcaa actggttaac caggagtgga aaacgttcct
    14281 cgacacccgt caccagggta cttttgatgt ggcccgtgca ggctggtgtg ctgactacaa
    14341 cgaaccaact tccttcctga acaccatgct ttcgaacagc tcgatgaata ccgcgcatta
    14401 taagagcccg gcctttgaca gcattatggc ggaaacgctg aaagtgactg acgaggcgca
    14461 gcgcacagct ctgtacacta aagcagaaca acagctggat aaggattcgg ccattgttcc
    14521 tgtttattac tacgtgaatg cgcgtctggt gaaaccgtgg gttggtggct ataccggcaa
    14581 agatccgctg gataatacct atacccggaa tatgtacatt gtgaagcact aatggcaata
    14641 cgtggggcag gagtgtcctg ctccacggtg tctgattttt atcgcattac agaaggcaca
    14701 ggccagaagg tagggcaatg ttaaaattta ttctacgtcg ctgtctggaa gcgattccga
    14761 cgctatttat tcttattact atttcgttct ttatgatgcg cctcgcgccg ggaagccctt
    14821 ttaccggcga acgtacttta ccgccagaag tgatggccaa tatcgaagcg aaatatcatc
    14881 ttaatgatcc gatcatgaca cagtatttca gctacctgaa acaactggcg cacggtgatt
    14941 tcggtccatc gtttaaatat aaagattatt cggtcaatga cttggttgca tccagttttc
    15001 ccgtttctgc caaactggga gccgcagcat ttttccttgc ggtaatactg ggtgttagtg
    15061 ctggcgttat tgccgcatta aaacaaaaca ccaaatggga ctataccgtg atggggctgg
    15121 caatgaccgg ggttgttatc cccagttttg tggttgcacc attattagtc atgatatttg
    15181 cgatcatttt gcattggctg ccgggcggtg gctggaatgg tggggcgctt aaattcatga
    15241 tattgccgat ggtggcgttg tcactcgctt atatcgccag tattgcgcgt attacccgtg
    15301 gctctatgat tgaagtatta cactccaatt ttattcgtac tgcccgggcg aaagggttac
    15361 ctatgcggcg gatcatttta cgtcacgcat taaaacctgc tctgttaccc gtgctctctt
    15421 atatgggccc cgcatttgtc ggcattatta ccggttctat ggttatcgaa accatttatg
    15481 gtttgccggg gattggacaa ctgttcgtta atggcgcatt gaaccgtgac tattccttag
    15541 tgttaagcct gaccatcctg gttggtgctt taaccatttt gtttaatgcc attgtcgatg
    15601 tgctatatgc ggttatcgac ccgaaaatcc gttactgata ctggagctcg cgatgatgtt
    15661 aagtaagaaa aacagcgaga cgctggaaaa tttcagtgaa aagctggagg tcgaagggcg
    15721 cagcttgtgg caggacgcac gtcgacgttt tatgcataac cgtgcggcgg ttgccagtct
    15781 gatagtgctg gtgctgatcg cgttatttgt aatcctggca ccgatgcttt cccagtttgc
    15841 ctatgacgat actgactggg cgatgatgtc cagcgccccg gatatggagt ccggtcacta
    15901 ctttggtact gactcatccg gtcgcgacct gcttgtgcgc gttgcgattg gcgggcgtat
    15961 ctcactcatg gtcggtgttg ctgcggcact ggtggctgtg gtcgtcggga cactttatgg
    16021 ttcgctttcc ggttatctgg gcggtaaagt ggattcggta atgatgcgtc tgctggaaat
    16081 cctcaactcc ttcccattca tgttcttcgt cattttgctg gtgacctttt tcggtcaaaa
    16141 catcctgctg attttcgtgg cgataggcat ggtttcctgg ctggatatgg ctcgtattgt
    16201 gcgtgggcaa accctgagtc tgaagcgcaa agagtttatt gaggcggcac aagttggcgg
    16261 tgtatcgaca tcgggcattg ttattcgcca cattgtgccg aatgtactcg gtgtggtggt
    16321 ggtctacgca tcacttctgg tgcccagcat gatcctcttt gaatctttcc ttagcttcct
    16381 ggggttgggt acgcaagagc cgttaagcag ctggggtgca ttgctgagtg atggcgcgaa
    16441 ctcgatggaa gtctctccat ggttattgtt gttcccagcg ggattcctcg tggtgacgct
    16501 gttttgtttc aactttatcg gcgatggctt gcgtgatgcc ctcgacccga aagatcgtta
    16561 aggagtgcag ccatgagcgt aattgaaact gcaactgtgc cgctcgcaca acaacaggct
    16621 gacgcactgc tgaacgtgaa agatttgcgt gtcaccttta gtaccccgga cggcgacgtc
    16681 acggcggtca atgatttgaa tttttcccta cgtgccggag aaacgctggg tattgtaggt
    16741 gagtctggtt cgggtaaatc gcaaactgca tttgcgttga tgggcctgtt ggctgccaac
    16801 ggacgtattg gcggatcggc aaccttcaat gggcgtgaaa tcctcaattt gccagagcat
    16861 gaactcaata aactgcgcgc tgaacaaatc tcaatgattt ttcaggaccc aatgacttcg
    16921 ttgaatccct atatgcgcgt cggtgagcag ttgatggaag tgctgatgct gcataagaac
    16981 atgagcaaag ctgaggcgtt tgaagagtcg gtgcggatgc tcgatgcggt aaaaatgccg
    17041 gaagcgcgta aacgcatgaa aatgtacccg cacgaatttt ctggcggcat gcgtcagcga
    17101 gtcatgattg cgatggcctt gttatgtcga cctaagctgc tgattgcgga tgaaccaaca
    17161 actgcgctgg acgtcaccgt acaggcgcag atcatgacgc tattaaatga actgaagcgg
    17221 gaatttaata ccgccatcat tatgatcacc cacgatcttg gcgtggtggc ggggatctgc
    17281 gacaaagtgc tggtaatgta cgccgggcgc acgatggaat atggcaacgc gcgcgatgtc
    17341 ttttatcaac ccgttcatcc ttattctatc ggtttgctca acgcggtgcc gcgtctcgat
    17401 gcggaaggtg aaacaatgtt gaccatcccg ggtaatccgc caaacctgct gcgattaccg
    17461 aaaggttgcc cgttccagcc acgttgtccg catgcgatgg aaatttgtag tagcgcaccg
    17521 ccgctggaag agtttacgcc tggccgtctg cgtgcttgct ttaaaccggt ggaggaactg
    17581 ttatgaatgc tgtaactgaa ggaagaaaag tcctccttga aatcgccgat cttaaagtgc
    17641 actttgaaat caaagatggc aaacagtggt tctggcaacc gccgaaaacg ctcaaagccg
    17701 tcgatggtgt cactcttcgc ctgtatgaag gggaaacatt aggtgtggta ggggaatcgg
    17761 gatgcggtaa gtccaccttt gctcgcgcca tcatcggttt ggtcaaggcg accgacggtc
    17821 atgttgcctg gttaggtaaa gagttgctgg gcatgaaacc cgatgaatgg cgtgccgttc
    17881 gcagtgatat tcagatgatt ttccaggatc cgttggcatc gctaaacccg cgtatgacca
    17941 tcggcgagat catcgctgaa ccactgcgta cttatcatcc gaaaatgtca cgccaggaag
    18001 ttcgcgagcg cgtgaaggcg atgatgctga aagtcgggtt attgcctaac ctgattaacc
    18061 gctatccgca tgagttctct ggtgggcagt gccagcgtat cgggattgcg cgtgctctta
    18121 ttcttgaacc gaagctgatt atctgtgatg agccggtgtc ggcgctggac gtgtcaattc
    18181 aggcacaggt ggtcaacctg ctccagcaat tgcaacgtga gatgggattg tcattaattt
    18241 ttatcgctca tgacctggcc gtggtaaaac acatttccga tcgtgtgttg gtgatgtatc
    18301 tcggccatgc ggtagaactg gggacctatg atgaggtcta ccacaatcca ctacatcctt
    18361 acaccagggc attgatgtcg gcagtcccca tacctgatcc ggatctggag aagaacaaaa
    18421 ccatccagtt actggaaggg gaattaccgt cgccgatcaa cccgccttcc ggttgtgttt
    18481 tccgtacccg ttgcccgatt gccggtccag agtgcgccaa aacacgtcct gttctggagg
    18541 ggagtttcag acacgccgtt tcttgcctga aagtcgatcc gctttaaaaa caataagggc
    18601 tgacagttgt cagccctttt tcacgctaaa agcgattatt tattcccgcc agatgatatg
    18661 gcaaagtttg tgatcttttt cgcggcataa cagaatgcgg gcaaaaacat cgttgatttc
    18721 accatcttca ctgtccgcca gaccaatcac cacttcggca aaaaagtccg ggttcaaatc
    18781 gaaatcaaca tgttcctgcc agtcttccgc cggatcgaat aactccgcgc cgccgcgctc
    18841 ttcaaactga agattgaaca gcagaacatc agctggatcg agattgtcgg cagccagttc
    18901 gagaaaaata tcgtaggcct gttcaagcgt ttcatcttca gtcaggcgat tgtttagatc
    18961 catatccata gttactacct gtttaacctc tgttggcgac gttttacagc aacggactga
    19021 agaagtaaaa cagtcgctcg gcgacacgtt gccataatgg acgttttagc cataaacggg
    19081 catcgagcag acgtgaacgc gaaatataat cgtcctgaac ggcggcgagg tcagcaccaa
    19141 aacctttatc gtcgattgcc agggtaatct cgaaatttag ccacagacta cgcatatcaa
    19201 ggttaactgt gccaaccaga cttagttcgc catcgaccag cacgctcttg gtatgcagta
    19261 acccgccttc aaactgataa attttaaccc cagcagccag cagttccgta aagaatgcgc
    19321 gactggccca gccgaccagc atcgagtcat tttttcgcgg aaggataata ctgacatcca
    19381 ccccgcgctg cgccgccgtg caaatcgcat gaagtaaatc atcgcttggc acaaagtagg
    19441 gcgtggtcat gatcaaatat tcacgcgccg aataagccgc agtcaataat gcctggtgaa
    19501 tgagatcttc cggaaagccg gggccagaag caattgtgtg aatggtgtga ccgctggcct
    19561 gttcaaacgg cataatattg acatctggtg gtggcggcag aatacgtttt ccggtttcaa
    19621 tctcccagtc gcaggaataa ataatcccca tcgcggtggc gatagggcct tccatacgcg
    19681 ccatcagatc aatccattgc cctacgcccg catcttgttt gaagtagcga ggatcgacca
    19741 tattcatgct gccggtgtac gcgatgtaat tatcgatcat gatcatcttg cgatgttggc
    19801 gcaggtccat acggcgtaaa aacacacgca tcagattgac ctttaaggct tcgaccactt
    19861 caataccggc attacgcatt agctcgggcc acgggctgcg gaaaaaagcc acactcccgg
    19921 cggagtcgag catcaatcgg caatgaatgc cgcgtcgcgc agccgccatt aatgattcag
    19981 ccacctggtc cgccatgccg ccgggctgcc agatataaaa caccatctca atattatggc
    20041 gcgcgagctg gatgtcgcgg attaacgcct gcatcacatc atctgactcg gtcatcagtt
    20101 gtagctgatt ccctttgacc ccagcgatcc cctgacgacg ctcgcaaagc ttgaataatg
    20161 gcgcagcgac actgctattt tcttcggcga agatatgctt acaggcttta aggtcgttaa
    20221 gccattttgc ggtggaaggc cacatcgctc tggcgcgctc agcgcggcgt ttgcctaaat
    20281 ggagctcgcc aacggcaaga taggcaataa ttccgactaa cggcagaatg taaataatca
    20341 acagccaggc catcgcggag ggaactgcgc gtcgtttcat tagaatgcgt aaagttacgc
    20401 ctgcaatgag caaccagtat cccagaatgg ccaaccaact caccaacgta taaacggttg
    20461 tcatagatta gaaatccttt tgaaagcgca ttgttatgag tttacgcata tggattcatc
    20521 tggcaaataa aaacgcggga aaagcactgg tcagcgtcgg gggtgggggt ataatgacca
    20581 ttctgttatt gcatagagta gttaacatga agcggagtag aacggaagtg gggcgctggc
    20641 gcatgcagcg tcaggctagc cgacgtaaat cgcgttggct tgaggggcaa tcgcgccgaa
    20701 atatgcgtat ccacagcatc aggaagtgca ttctaaacaa acagcgtaac tcgttattgt
    20761 ttgcgatcta caatatctaa atgtactggg caccattgcg gtgcccaata agtttactct
    20821 tccgtcttta aactctattt ttgcgccgat tctttactgt cagctttcgt ttctagttca
    20881 tcgttatcgc tgaaaatacc atgacctgag ttcaacaaca ttgaaacgag catatcatta
    20941 ttaatctctt caccatttaa aactcgcgcc agaatttcgc tgccaaacag ttgcggtgag
    21001 agaatgatat ccggatgtac catcttaatc ttattcaggt ttttgctatc actgacggcg
    21061 agaactgttt tgacatcact gctcatatct tttgccgaga gtacaacaaa cgcgttatct
    21121 gcatcgttat cactcagcgc cagaatggcc cggcaacgat cgattcccgc tttctttaat
    21181 acagaactgt cattactgtc accggggata acatcagcat tatcgcctaa gcgttgctca
    21241 agttgcttga tatcatcttc aggcaagttg ctgataaccg ttacgttttg tccgcgttga
    21301 ttcagttgca ggatcgtatt gatggcgaga atcgaatgtc cgcaaacaat aaaatgatct
    21361 ttacgatgca ttgtatgatt gtttcctttt acaagtttgt tgaatccccc gcggataagc
    21421 ggaccaaaaa ttgaggtcat agatgtggca aaaacggtaa tgccggaaat aatgaccgaa
    21481 atagtgaaca atcgtgctga ttcagaaaca gggacaatat cgccatagcc gacggttgac
    21541 atggtttcta tcgaaaaata gaacgcggtc atcaaacttt ctattcgcgg attaaaacct
    21601 tcgcttaaat aaagcgcacc gtaggttgag taaaacagta atgtcgtgaa actaataaat
    21661 gcaaaaattg tcccggctgc ggcgctactg tgggagaagt ctttgcgcag tatcagcaaa
    21721 aacaccagcg taaaaatgca aaatccaata ctaaatttca gccagggata aaaatgcagg
    21781 gtgtaaatta gcgctatcaa caacaaaatg atactgattg cccatgcgag ctttgcccgg
    21841 aacagtaagc caatggagtt caggacaaga aagacgccga gcataaagag tggggcgttt
    21901 gccagcaagg agagatccaa ctctgaaaat gctttgatat ggaaaatatc aagcaaattg
    21961 accgacatac catagattgt tttaaaaata agcaatccat ttaaaaagac ggccagcgcg
    22021 agaatgtcgt gccgaagggt cacccataaa tttgttgctg tttgtttgaa tgtagcccag
    22081 tgactcactg atacctctgt aaccacaatt ttgtttaatt atcttagcgt gcacattttc
    22141 tgaacataca tgcagcgcgt gaatgtgtta aacaatagta gatgacttta tccgcagtaa
    22201 tcaaaggatt tgagcgagca actgtacctc aggaaaggtc atttttcctg aatatgctca
    22261 catcatataa agaaatacag ataaagttat tatctgcttg tggtggtgaa tgcactgacc
    22321 ggctataagg aaaggccaaa caagaacacg gttgcaaaaa ccgtgccctt aaatattgaa
    22381 tctctattca gaacactttc ttaaatggtt tcactgaaac gtgttcatag actcctgccg
    22441 ctacgtacgg gtcagcatcg gcccaggcct gagctgcttc cagcgattca aattcagcaa
    22501 taacggttga gccagtaaat cccgcagccc ctggatcgtt actgtctacc gctggcattg
    22561 gaccagctgt caacaaacga ccttcatcat gcagtaactg taaacgtgct aaatgtgccg
    22621 gacgaacgga aaggcgtttt tcgaggctat cagctttgtc ttgagcgtaa ataacataca
    22681 acacgggcac aactccttgt tcgggaaagt tgtaaagtac gttatttgaa agggcgaaga
    22741 tctgcaacgg aaagatgatg tctttgttaa ggccatgcat aaagtaaggg taattacgcc
    22801 aaaaatgaca ttttcactga tcctgatcgt cttgccttat tgaatatgat tgctatttgc
    22861 atttaaaatc gagacctggt ttttctactg aaatgattat gacttcaatg acccttgatt
    22921 tacctcgccg cttcccctgg ccgacgttac tttcggtctg cattcatggt gctgttgtgg
    22981 cgggtctgct ctatacctcg gtacatcagg ttattgaact acctgcgcct gcgcagccga
    23041 tttctgtcac gatggttacg cctgctgatc tcgaaccgcc acaagccgtt cagccgccac
    23101 cggagccggt ggtagagcca gaaccggaac ctgagccgat ccccgaaccg ccaaaagaag
    23161 caccggtggt cattgaaaag ccgaagccga aacctaagcc aaaaccgaag ccggtgaaaa
    23221 aggtacagga gcagccaaaa cgcgatgtca aacccgtaga gtcgcgtccg gcatcaccgt
    23281 ttgaaaatac ggcaccggca cgcctgacat caagtacagc aacggctgca accagcaagc
    23341 cggttaccag tgtggcttca ggaccacgcg cattaagccg taatcagccg cagtatccgg
    23401 cacgagcaca ggcattgcgc attgaagggc aggttaaagt taaatttgac gttacgccgg
    23461 atggtcgcgt ggataacgta caaatcctct cagccaagcc tgcgaacatg tttgagcgtg
    23521 aggtgaaaaa tgcgatgcgc agatggcgtt atgagccggg taagccaggc agtgggattg
    23581 tggtgaatat cctgtttaaa attaacggca ccaccgaaat tcagtaagca gaaagtcaaa
    23641 agcctccgac cggaggcttt tgactattac tcaacaggta aggcgcgagg ttttccttca
    23701 ggatcaaccg cgacatactt aaataatgct tctgtcgctt tatagcgttg cccaattggt
    23761 tcagacgcta cttttttcac ccacacttca atattaatgc tgaccgatgt cgtccctttc
    23821 tggacacagc gtgcatagca gcacaccaca tcgccgaccg caaccggccg taagaaagtc
    23881 attccttcaa cccgcacagt cactacgcga ccgtgggcaa tttcttttgc cagaatagcg
    23941 ccgccaatat ccatttgtga cattaaccaa ccaccaaaga tgtcaccatt ggcattggta
    24001 tcggcgggca tggctaaagt acgtaaaaca agatcgccct gagggacgtt atgtgttgta
    24061 gacatggtaa aaccgactta aaaggaaaat cacaggcgcg atgctactat gattttcctg
    24121 aggagaacag aggagataca gcgccagccc cgaaggactg gcagtctggt taggatttat
    24181 cttcctgcgg catgtggcgg tagatataga taccgcttaa caatgtgaag attaaggtaa
    24241 gggcggtcag gccaaagact ttaaagttga cccaaatatt ttgcggcagc cagaatgcga
    24301 tgtagatgtt tgccagaccg caaaggataa agaaaacagc ccaggccaga ttcagcttcg
    24361 accataccgg ttgcggcagc gtgagttctt tacccagcat ccgctgaatt agcggctttt
    24421 tcatcaccca ttggctgact aacagggctc ccgcaaatag agcataaatg actgtaacct
    24481 tccatttaat aaactcatca ttgtggaaga acagcgtcaa gccaccgaag acgaccacca
    24541 gaacaaaagt gatcagggcc atcttctcaa ccttacgaaa gcgaacccag ctatagataa
    24601 gcacaatcgc cgtggcgacg atcagcgccg cagtagccgc atagatgtca taaatcttgt
    24661 aaaacgcgaa aaagacaacc agcggtaaaa aatcaagaaa ctgcttcatt ttacgattcc
    24721 gtaatcaaat gcttaaggtt attggcgaat caacatatac aggcggaaca gatagatgag
    24781 caatatggct gaaatcaggt tgctcaaggt gttcgccagt acggcaccaa tttccggggt
    24841 taatgcggca aaagaagagg caaaaagcag caacagtgtt tttgccagca accagctcag
    24901 tactgcgggt gccaccagac gcatattcgc ccaagtcagc cgcatactgc tacgcatcga
    24961 ggcaaaaacg cccattttgt cctgtaccaa catcaccggt gccagggcca gtaaaatggc
    25021 catgataatt cccggaacga ccaccagcat gatgccaatc tgtactaaaa gggtagtcag
    25081 aaaaatcaga ataaataact ttggcaatat cggcgcactg gcaccaatag ctcgcagcgc
    25141 actgactctc tgacccgcag acaccagctg gataattaat attacgcctc cggcgagaat
    25201 ggcgttaccg attaatcctg aaaaagtgga cgccgctgaa gcctgcagca aaatttgttg
    25261 ctgttccggt gacatattct gaaccaggtc gaacaaccca ctactgccgc taacgggcac
    25321 gccgtcattg agctgcgcaa gctgtgcatc actgggtgag aaaacatgcc ctaacaccac
    25381 tgtgataaac gcacatagca acgataccaa cagaatggtc ataaattgat tacggaagaa
    25441 atttccggtg tcacggtata cggactgcgc cgtgatagac atgcactctc cttgagtttt
    25501 atgcaggtgt taattagcgg gcaattgtac cctggttaag cctcgggtgg cagcatcagg
    25561 tcttgtatgg aaaagcatat ctttgtaaag cggaggtaat tgctggcctt caggcatggg
    25621 caaatcgcgg caagtggcga ccgagttaat atttgcgtag cgaaaatatt taaaaattga
    25681 tttaaatcac attaaccagg attctcaatg caacttctaa attaatccag atcaataaag
    25741 ggtgaattat catatgtaat gtgatctatg taggatcatt tgttactcca atgtaggtat
    25801 attcgtcacg tttttataac cataacgacg gagcggatat gaaaaagtta acagtggcgg
    25861 ctttggcagt aacaactctt ctctctggca gtgcctttgc gcatgaagca ggcgaatttt
    25921 ttatgcgtgc aggttctgca accgtacgtc caacagaagg tgctggtggt acgttaggaa
    25981 gtctgggtgg attcagcgtg accaataaca cgcaactggg ccttacgttt acttatatgg
    26041 cgaccgacaa cattggtgtg gaattactgg cagcgacgcc gttccgccat aaaatcggca
    26101 cccgggcgac cggcgatatt gcaaccgttc atcatctgcc accaacactg atggcgcagt
    26161 ggtattttgg tgatgccagc agcaaattcc gtccttacgt tggggcaggt attaactaca
    26221 ccaccttctt tgataatgga tttaacgatc atggcaaaga ggcagggctt tccgatctca
    26281 gtctgaaaga ttcctgggga gctgccgggc aggtgggggt tgattatctg attaaccgtg
    26341 actggttggt taacatgtca gtgtggtaca tggatatcga taccaccgcc aattataagc
    26401 tgggcggtgc acagcaacac gatagcgtac gcctcgatcc gtgggtgttt atgttctcag
    26461 caggatatcg tttttaattc cgcacaaaaa cgaccccgta atatacgggg tcaataagga
    26521 catggtataa agggtattat ttcttcgctt ctacgccatc agtttcagag cgaattaaaa
    26581 atttctcagt tgtttgcgga atattctgaa tcagccagtc ggccatttgc ttttcctcat
    26641 ttaaaatcgc ttcgatggtt ggaattgaag ctgtatcacc ggcatttttt gctgctgcta
    26701 atagtgaggt gtaacaggcg atttcaaatt gctcgaagac atatccgcta atagagcctt
    26761 tgactatttc atcagaaggg aatataccac cgattgactg cccaagcgca gccattttac
    26821 tcatggaatc tttaatgact gaacgtgaaa tgtcattacg atcaagaata gtttccagtt
    26881 gaacaatctg gtttttggtt tcactaagat gttgttcaat acgagcgcgt agttcaggat
    26941 aattatctat acggctggcc atggattcaa gcatagattc ggcttgcttt tccattgcgt
    27001 gggcgtcacg taaccagtca tgataatgtt caatacgatt catgaaaatg tcctctttat
    27061 aaaatgacta aaagttaaaa ttcatatttc aggctttatt ttcggctttc ttatttacgt
    27121 tattaatggc cagatcagtc agtttgatgt cggtggcctt ttcttcttcc agggtttctt
    27181 tcagaagctt cgctgcttta cggtaaccta attgttcagc cagcgtcgct aatgtcccgt
    27241 aactggcaat ctcataatgc tcgactttct gtgctgcggc aatcagtgcg gcatcacgca
    27301 cttcgttttt ctcggtactt tcgatgacct cattagcttc ttcaataaga ccttccattg
    27361 ccacacattt catgcgctta attttcaggt tcgattcgga ctccacaact tgatcaatac
    27421 gttcaatctg tccatgagtt tcctcgaggt gcgcatgaaa agcctgactt aatttttcat
    27481 ttgatgttgc tcttgcgagt tttgccagtg cccgggttaa ttgtttttct gcgctgtagg
    27541 tatctgaaag caggtgaata aatacatctt caatggtctt catattcata tttttctcca
    27601 gtgaaatcac tgcgagccat acggcccgca gatgcaatta tctttatcag ctgatattaa
    27661 taattaacgg tgaatcagga tttgcctgat ttattaccac cgctttgttg accgcctttt
    27721 ttacccgctt cagatgcgcg ttgcggatca tttttaaaat taccgccgct atgctgaccg
    27781 cctttacggc ctgcgtcgga tgccttctca cggtcttcgg cgaaatttcc tgaaccacca
    27841 cgatgttcgg ccatgttatt tctcccgttg cgttgcattg tttcattaat atgagtgttg
    27901 tgtgtcgaca ctcattaaaa ttagtcgcta atgagaatta gtcaaattaa gcgcaacgag
    27961 aagatagagg gaaaatatat tttgaggaac attctggata tattaacaat tacctgagga
    28021 ataagtgact tagagaaaat tattgatttt actggtgtta tgttgcggga ttaatttgtt
    28081 tgattatatt actgttgggc ggaaaaatga cgtaagttga cgttcgaccg gggtaagcga
    28141 aacggtaaaa agataaatat taaatgaatt taggattttt ccggcttcat taaagaaagt
    28201 taaaatgccg ccagcggaac tggcggctgt gggattaact gcgcgtcgcc gctttcatcg
    28261 gttgtacaaa aactttcagt gccgccagca ttttctctgg ctcattaata tgttgctcga
    28321 tgattttaac aatggccgaa ccagaaatcg cgcccgcagc tcctgcatca atcgctgctt
    28381 ttacctgatc cggggcggaa ataccaaatc cctgcaatgg aggtgcagcg ttgtactctt
    28441 tcagcttcgc aaccagatga ttgaggggta acgcggcgcg gttttctgcg ccggtcacgc
    28501 ctgctcgtga cagcaaatag gtgtaaccac gaccgtaaga ggctatctgg cgcagcaggt
    28561 cgtcatcggc atttggcggg cagatgaaga taggtgcgac attatgacgc aacgcggcct
    28621 ggcggaaggg cgcggactct tcaactggca catcggcaac cagcaccgaa tcgacgccga
    28681 ctttttcgca ctgggcataa aactcatcaa tgcctttgtt aaacaccaga ttggcataca
    28741 tcaacaggcc aatgggaatg gtcgggtgtt tctggcgaat cagtgccagc atttcaaaac
    28801 attgtgccgg agtcacacct gccgcaaagg cgcgcagagt ggcgttttga atcgtcgggc
    28861 catccgccag tgggtcggag aaggggatac ctaactccag cgcgtcagca ccggcttcaa
    28921 ttagcgtatc gataattttc aatgactgct caatgcccgg atcaccgagc gtgacgaaag
    28981 gaacgaatgc gccttctttg cgctccttca actgggcaaa cagagattcg tagcgttcca
    29041 tcagatttcc cctcgtgctt tcaaaatatc gtgaacggtg aagatgtctt tatcgccgcg
    29101 accggaaagg ttaaccacca gtagctgctc tttatccggg ttttcgcgca tcattttcaa
    29161 cgcatgggcc agggcgtggg aggattccag cgccgggatg atcccttcgt gcaggcacag
    29221 cgttttgaag gcttcaaggg cttcatcatc ggtaatagac acgtaatcag cgcgtccagt
    29281 gctgttaaga tacgcgtgtt gtgggccgac agacgggaaa tccagtccgg cggagatgga
    29341 gtaagattct tcaatctgcc cgtcttcggt ttgcatcatc ggcgctttca taccgaaata
    29401 gatacccacg cgaccatgtt ttagcggtgc gccgtgctcg ccagtttcga taccgtgacc
    29461 acctggctcc acaccaatca ggccgacgtt ggtttcattg atgaaatcag caaacatgcc
    29521 gatggcattc gaaccgccgc caacacaggc gataacggca tccggcaggc gaccttctct
    29581 ttccagaatc tgcgctttgg tttcttcgcc aatcatccgc tgaaactcac gcacaatggt
    29641 cggataagga tgcgggccag ctgcggtgcc cagcatatag tgcgcggttt cgtaactacc
    29701 ggaccagtcg cgcagcgcct cgttacaggc atctttcagc gtcgcggaac cgctatgcac
    29761 cgggatcact tccgcaccca ttaagcgcat acgaaaaacg ttaggcgact ggcgttcaac
    29821 gtctttggca cccatataaa tacggcattt caggccgagc agggcgctgg caagggccga
    29881 cgccacgcca tgctgaccgg caccggtttc ggcgatgatt tcggttttac ccatccgctt
    29941 cgccagcaac gcctgcccca gcacctggtt agttttatgc gcgccgccgt gcagcaaatc
    30001 ttcacgcttg agatacagcg tggtgttcgt cccggctgta atgttctggc atttggtcag
    30061 cgcggttgga cgcccggcat agtttttcag caggtcgttg aactgagcct gaaattcagg
    30121 atctttttgc gcactgacaa aagcttcttc cagctggcgc agagcaggca tcaggatttg
    30181 tggcacgtac atgccgccaa actcaccaaa ataggggtta agtaatgttg tcattgttcc
    30241 tttccttaat atgcgcgcag cgtctggaaa accgaggcca aaagacgtgc gtctttgatg
    30301 cccggttgcg actctacagc agaattaaaa tcaagtccgg cgcagccggt ttgtgccgct
    30361 tccacgcagt tatctgtgcc taagcccccc gccagcagaa cgttgccaag cgattgacca
    30421 tttaatagtg accagtcaaa acgttgcccg cttccaccct ggccgttgtc taaaacatat
    30481 ttatcaacgt gctgaaactc gcgggcgggc agggtttcac cgacgcttaa tgctttccag
    30541 atggcaacat gtgctggcag agcttcacgc agcgtatcga tatacagctg ttcttcatta
    30601 ccatgcagtt gcactgccgc cagcgataac accttagctt tgtccaccac atcggcaata
    30661 tcgtgattgc ggaacacgcc aacatactgc aacggtgccg cagccatcac ttcctgcgcc
    30721 tgttcaacgt tgacgcaacg cggtgatgtc gcaacaaaaa tcaacccacc gtaaatcgcg
    30781 cccgcgtcat aagctgcttt agcatcttgc ccacgcgtca ggccacatac tttattctca
    30841 cccagcaaca cccggcgcac ggcggcgtgc aaatcgtcat gggccatcaa cgccgaacca
    30901 atcagaaaac cgttagcgaa gtggcttaac tcgcgcacct gagcgtaagt attgatgccg
    30961 gattcgctga ttaccgtcac gttgtgcccc agtttcggcg caagctcgcg ggtacggttg
    31021 agatcaatcg acaaatcacg cagatcgcgg ttgttgatgc caacgacctt tgctcccaat
    31081 gcaatggcgc gctcctgttc ctcttcatta ctgacttcgg tcagcacccc catctccaga
    31141 ctgtgagcga cggcggcaag ctggcgatat tggtcgtcat ccagtactga aagcattaat
    31201 aagcaggcat cggcctggta atagcgcgcc agatagatct ggtaagggtc gataatgaag
    31261 tctttacata aaatcggctg cggggcgatt tggctgacga tggggaggaa attaaagctc
    31321 ccctgaaaat atttctcatc agtcagcacc gaaattgccg aagcgtaatg tttataaatg
    31381 gcggcaatgc gtgctggatc gaaatcatca cggatcacgc cttttgacgg cgacgctttc
    31441 ttgcactcca gaataaacgc cgtgcgcgca ccctgtagcg catcataaaa atgtcgcgtg
    31501 ctcggctgaa cctcattctg aaaactggcc agcggttgct gctgtttgcg ggcttctacc
    31561 caaatcgcct tgtctgcgac gattttcgct aaaacggttt gcatcattta ccctcgtgcc
    31621 gccagtgcgg tgactctgtc gtaagcggaa ccactgcgca gtacctcaag aacggtttgc
    31681 gcattggctt gcagatcttc atggccatgc aggcgcatta acatggcgac gttcgcagcg
    31741 acggctgctt catgggcggc gtcgccttta ccttgtaaca aacgtgttaa aatgtcacgg
    31801 ttttcttccg gtgttccgcc tgccagttgc tcctggtggt agggtgtcag gccaaagtct
    31861 tctgcggtga gctgatagct tttaatttcg ccgtcatgca gttcggcaac gattgtcggc
    31921 gcgtgtaatg aaacttcatc catcccgccg ctgtgcacca ccgccgcgcg ttgatacccc
    31981 agcacgcgca aggtttcggc aatcggcagc accagttccg gactataaac accaattaac
    32041 gccagcggcg gatgcgccgg gttaatcaat ggccccagca cattgaacag ggtgcgggtt
    32101 ttcagttgct ggcgaaccgg catcgcgtgg cggaatccgg tgtgatactt cggcgcaaag
    32161 aggaaacata cacctaactc atccagcgcc tggcgcgatt tatcggcgtt catatcaaga
    32221 ttaataccga acgccgccag cagatcggac gaaccagatt tactggagac gctacggttg
    32281 ccgtgtttcg ccactttcag cccacaggcc gcggcgacaa acgcactggc ggtagaaata
    32341 ttgatactgt tgctgccgtc accgccagta ccgacgatat cagcaaacag ataatccggg
    32401 cgcgggaacg gcgctgcgtt ttccagtagc gcggttgctg ccccggcgat ctcgttcggg
    32461 tgctcaccgc gaattttcat gctcaccagc gccgccgcca gttgttccgg cttcagttcg
    32521 ccacgcacca ccgctgaaaa cagctggtgg ctttcttgtt ggctaagcgt ctgcgcctga
    32581 tacagttttt ccagaatcgg ttgcagcgtg ttggctggct ctagtttctg ctgcgcccag
    32641 gccagcgttt gttccagcag gcgagcgccc tgggtggtga gaatggattc cggatggaac
    32701 tggaatccac aaacgcgatc cgcatcgtga cgtactgcca tcaccatgcc attaaaatgg
    32761 gcgttgatgg ttaaaccggc cggaatgtta ctgccaacca gcgagtgata acgcgccacc
    32821 ggcagcgggt ttgttaatcc ggcaaacatc gcctgaccgt catgttcaat gctggaggct
    32881 ttaccgtgga gaatttcgcc cgcctgaccg acatagcccc cgtaagcttc gacaatcgcc
    32941 tgatgtccga ggcaaatgcc aataatgggc agcttgccac gcaagcgggt gaggagttcc
    33001 ggcatacaac cggcttcgct cggcacaccg gggccaggag aaagcatcag caccggattg
    33061 ctcatggtcg ccaggcgttc aattaaggtt tgcgccggaa tatggttgcg gtaaatcacc
    33121 acgttatgcc cattgctgcg caactgatct gccaggttgt acgtaaaaga gtcgatatta
    33181 tcgagcagca gaatgtcagc catcagaaag tctcctgtgc atgatgcgcg gtggcaatag
    33241 cgcgcagtac agcgcgggct ttgttacggg tttcgtcggc ttccgactgc ggaacagaat
    33301 caaggactac accagcaccc gcttgcacgg tggcgatacc gttttccacc agcgccgagc
    33361 ggatcacaat gcaggtgtcg agatcgccat gcgcggtgaa ataacctacc gcgccgccgt
    33421 agctgccgcg gcggcgacct tccgcctcgg caattaactg catagcgcgt actttcggcg
    33481 caccgcttaa cgtccccata ttcatacagg cgcgataagc gtgcagggcg tcaagatcgt
    33541 gacgcagttc gccgactacg cgagagacga ggtgcatcac ataggaataa cggtcaactt
    33601 tggtgagatc ggcgacgtag cggctgccgg gggtgcaaat gcgtgccaga tcattacggg
    33661 cgagatcaac cagcatcaga tgttcagaca gctctttatg atcggtacgc atttccagtt
    33721 caatacggct gtcgagatct ctgtccagtg aaccatcggc gcgacgaccg cgtgggcgtg
    33781 ttccggcaat cgggtagatc tcaatctggc ggctggtggc atcatacttg agcgagcttt
    33841 ccggcgacgc gccaaatagg gtgaaatcat tatcctgcat aaaaaacatg tacgggctgg
    33901 gattactctt tttcagcacg taataggccg ccagcggtga cgggcagggc agagagaaac
    33961 ggcgagatgg caccacctgg aaaatttctc cagcgcgaat cgctttttgc aacaaacgca
    34021 ctacgccacc gaactcttca tcgctctgat tacattcaca acgcatatgc ggcacggaaa
    34081 ccactggcag cggcggcgcg gcttcggtca gttgctgacg tagttcgttc aggcgagcag
    34141 tgagacgttg tttttcttct tcattcggag caaacaggct ggcctgaata cgggtgcttt
    34201 ttttctgatg gtcaatcacc atcagcgttt cagcgagata aaaacagaaa tcagggcagt
    34261 tattttccgc tgacagttgc ggtaaatctt caaatcccgc cacaaggtca taagagaaca
    34321 ggccgccgaa gaacatggct tctcgttctt ccttcggtac attcaacaga ttctgcaata
    34381 aacggaaagc gtcaaaaacc gaaagggagc ataagcgggc gtcttcatcc agcagtggac
    34441 tgacaggggg gaagcgcagc acacggcagt ttggtgattg ttcactttcc acacccgcag
    34501 gcagggcgtt atccagtagt gccagcaggg cttcgccgtt gccggaaagt gcctggattg
    34561 tgacagtgtc acctaaagct gtaatgcgca gcgcactgtc taccagcagc aggcttttta
    34621 aatcatcttt gctgtcgata tctgcggatt ccagcagcag cgttgccgga cgatccccac
    34681 acaactggtg aaaaagcgcg gtgggattgt cgcgataagc gccttcgcag gttagcagtt
    34741 cgagagtcgg tttttgtgtt tgcattgtta ttctctaatt ttgttcaaaa aaaagcccgc
    34801 tcattaggcg ggctgggtat ctgattgctt tacgcatggt gaatacactg cccgtttcag
    34861 gaagtgcgcc accaaccttt cagtacgaaa attgctttca ttgtcgatac cctttttacg
    34921 tgaacttgcg tactagttaa ctagttcgat gattaattgt caacagctca tttcagaata
    34981 tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt
    35041 gagcgacacg aattatgcag tgatttacga cctgcacagc cataccacag cttccgatgg
    35101 ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgag atgcgcgtcg gcaccctggc
    35161 gatcaccgac catgacacca cagctgcaat cgcgccagca agagaggaaa tttcacgttc
    35221 gggactggcg ttgaatctta ttcccggcgt ggaaatttcc acggtctggg aaaatcatga
    35281 aattcatatt gtcgggctga atattgatat tactcatccg ctaatgtgtg agttcctcgc
    35341 gcagcagaca gaacggcgca atcagcgggc gcagctgatt gccgaaagac ttgaaaaagc
    35401 gcaaatacct ggggcgctgg aaggcgcaca acgactggcg cagggcggcg tagtgacgcg
    35461 cggtcatttt gcgcgtttcc ttgttgagtg cggcaaagcc agttcaatgg cggatgtctt
    35521 taaaaagtat ctggcgcgcg ggaaaaccgg atacgttccg ccacagtggt gtacaataga
    35581 acaagctatt gatgtcattc atcattctgg cggtaaggcg gtgctagctc atcctgggcg
    35641 gtacaatctt agtgctaaat ggctgaaaag attggttgcg cattttgccg aacaccacgg
    35701 tgacgcgatg gaagtcgcgc agtgccagca atcgcccaat gaacgtaccc agctggcggc
    35761 ccttgcgcgt cagcatcatt tatgggcatc acaaggatct gattttcatc agccatgccc
    35821 gtggatcgaa ctgggtcgta aactctggct gcccgcaggc gttgaaggcg tctggcagct
    35881 atgggaacag ccgcagaaca ccacagagag ggaattatga gccagttttt ttatattcat
    35941 cctgataacc cacagcaacg tctgatcaac caggcggtgg agatcgtgcg taaaggcggg
    36001 gtgattgttt atccaactga ttccggctat gcgctcggct gtaaaattga agataaaaac
    36061 gcgatggagc gtatttgtcg tattcgccag ctgccggacg gtcacaactt taccctgatg
    36121 tgtcgcgatc tttctgaact ctcgacctat tcatttgttg ataacgttgc gtttcgttta
    36181 atgaaaaaca acacgccggg caactatacc ttcatcctga aggggacgaa ggaagtgcca
    36241 cgccgtctgt tgcaggaaaa acgcaaaacc atcggtatgc gtgtgccttc taaccctatc
    36301 gctcaggcgt tacttgaagc attgggcgaa ccgatgcttt ccacttcgct aatgctgcca
    36361 ggcagcgaat ttaccgaatc ggacccggaa gaaattaaag atcgtctgga aaaacaggta
    36421 gatttgatta ttcacggcgg ctatctcggg cagaaaccga caacggttat tgatcttacc
    36481 gatgatacgc cggtcgtggt gcgtgaaggc gtaggtgatg tgaagccttt cttataacaa
    36541 attgcgttcc acggatggaa gactatggca gggaagtttc gctgcatttt gctgttgata
    36601 gcagggctgt ttgtatcatc tctaagttat gcagaaaaca cggagatccc ttcttatgaa
    36661 gaagggatct cgctctttga tgttgaagcc actctgcaac cagatggggt gctcgacatc
    36721 aaagaaaata ttcattttca ggcgcgaaat cagcagatta agcacggctt ttatcgtgat
    36781 ttaccacgac tatggatgca gcctgatggg gacgctgcac tgctgaacta tcatattgtt
    36841 ggcgtcaccc gtgatggtat tcctgaaccc tggcatcttg actggcatat cgggttaatg
    36901 agtattgtcg tgggcgataa acaacgtttc ttgcctcaag gcgactatca ttatcaaatt
    36961 cattatcagg ttaaaaatgc tttcctgcgt gagggggatt ctgatctgct aatctggaac
    37021 gtgaccggta accactggcc gtttgaaatt tataagaccc gtttttctct ccagttctct
    37081 aatattgcgg gtaatccatt tagcgaaatc gatctcttta ccggagaaga gggcgacaca
    37141 tatcgtaatg gccgcatcct tgaggacgga agaattgaat cccgcgatcc gttttatcgt
    37201 gaagatttca cggtcctcta ccgctggcct cacgctttac ttagcaatgc ctcggctccg
    37261 caaacgacga atattttcag ccatcttctt ttaccctcca cgtcatcgtt gttaatttgg
    37321 tttccgtgtc tgttcctggt ttgtggatgg ttatatctct ggaagcgcag gccgcaattt
    37381 acgccggtag atgtgattga aaccgatgtc attccgccag attacacgcc cggcatgtta
    37441 cgtctcgatg cgaagctagt ttacgacgat aaaggttttt gtgccgatat cgtaaatctg
    37501 attgtgaaag gaaaaattca tctggaagat cagtctgaca agaaccagca aatcctgatt
    37561 cgtgttaatg aaggcgcgac cagaaataat gcggtattac tgtccgcaga gcagttatta
    37621 ctggaagcgt tatttcgtaa aggcgataag gtcgttctta cggggagacg caacagagtc
    37681 ttacgcaggg catttttacg gatgcagaaa ttttatctgc cgcgtaaaaa gtcttcgttt
    37741 taccggtctg atactttttt gcaatggggt ggactggcaa tattggcggt cattctttac
    37801 ggtaacttga gtcctgtagg ctgggcagga atgagtctgg tgggcgatat gtttattatg
    37861 atctgctgga ttattccttt tttattttgt tcccttgagc ttttgtttgc ccgcgatgat
    37921 gacaagcctt gcgttaatcg tgtaatcatc actttgtttt taccactgat ttgttcaggc
    37981 gtggccttct attctctcta catcaatgtc ggagatgtat tcttttactg gtatatgcca
    38041 gcgggttatt ttaccgctgt ttgcctgacc ggttatctca ctggcatggg gtatattttt
    38101 ctgccaaagt ttacccaaac tggacagcaa cgttatgccc acggtgaagc tatcgttaac
    38161 tatcttgcgc gtaaagaggc agcaacacac agtggacgtc ggcggaaagg ggaaacacgg
    38221 aaactggatt acgcgttgct aggttgggct atatcggcaa atttggggag ggaatgggcg
    38281 ttacgcattg ccccttcgct ttcttcggcg attcgcgctc cagagattgc ccgtaacggc
    38341 gttttattct cattacagac gcacctaagt tgcggggcca ataccagttt gttggggcga
    38401 agttattccg gtggtggtgc tggcggcggc gcgggtggcg gaggcggtgg tggctggtaa
    38461 ttaagctgat gttaatcgaa acaagcttta accataaaga tagtgtagta tgttgcgcct
    38521 caaagccagg ccgataaacg tcgagtcgtt tacttaaggc ctgaagagtt caaacagact
    38581 ttactatcag tgagtcagat gtaagttctg actcactgtt gcacaaaagc atatcagacg
    38641 cctgggaagg cgacaccaaa aggaagctct atgagcgaaa agctacagaa agtgctggcg
    38701 cgtgccggcc acggttctcg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    38761 gttgatggca aaattgctaa actcggcgat cgtgttgaag ttacccctgg attgaaaatc
    38821 cgtatcgatg gtcacctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    38881 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg acgtccaacg
    38941 gtgtttgacc gtctgccaaa actgcgtggc gcacgctgga ttgccgtggg tcgcttggac
    39001 gttaatacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    39061 cacccaagcc gtgaagttga acgtgaatat gccgtgcgtg tatttggtca ggttgacgac
    39121 gcgaaactgc gtgatttgag tcgtggcgtg cagttggaag atggtccggc agcttttaaa
    39181 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    39241 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    39301 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    39361 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagctacc gccagaaacc
    39421 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggcgaatca gattcgtcgt
    39481 gcggtgaaac gtcacagtca ggtgagcggc ggtcgtcgtt ctggcggacg taataataac
    39541 ggttaatcaa agtatgccgg acgtcatatc cggcattttt acagattaat aatcgatccc
    39601 tatctgcgct tttacaccgg catcaaacgc atgtttgacg gggcgtaatt cacttaccgt
    39661 atctgccagt tcaagaatat cccgatgaca accacgaccc gtgataatca ccgtctgttg
    39721 atgtggacgt tcatttaacg cctgcaccac ttcttccagt ggcaaatagt catacgccac
    39781 catatacgtc agttcatcaa gcaaaaccat atccagtgag gaatcagcaa gcatccgctt
    39841 tgcatgttgc cagacttcgc ggcaggcggc ggtatcagac tcgcggtttt gtgtatccca
    39901 ggtaaagccc gttgccatca cctgaaactc aacgccatgt ggctccagca gattgcgttc
    39961 gccattaggc caggtgcctt taataaactg cacgacgcct actttttttc cgtgaccaac
    40021 tgcgcgtgtt gccgtaccaa atgccgcggt ggttttgcct tttccattgc cggtaaagac
    40081 gatgataata ccgcgttcat cctgggcctg ggccacacga gcatctactt tttctttcac
    40141 tcgctgctga cgctgttggt agcgttcatc actcattggg aaattcctgg tttacggccc
    40201 ggttgggcgt caaaggtcat gccggtttta cggcggctgt catcgcccat cagccagagg
    40261 tagagcggca tgatatcagc gggtgtttta agtttctgtg gatcttcggt cgggaaggcg
    40321 ctggcacgca ttgcggtgcg cgtaccgcct gggttaatgc agttgacacg caggcgctgc
    40381 tgatattcat cggccagtac ctgcatcatc ccttcggtgg caaatttcga cgctgcatat
    40441 gcaccccagt tggctcgtcc ctgacgtcca acgcttgatg aagtaaagac cagtgaaccg
    40501 gcgtccgatt tgagtaataa aggaagcagt gcctgggtga gcataaaggt ggcattaacg
    40561 ttgacctgca tgacgtcctg ccagacctgc ggattttgtt cgctcattgg gcaaacatcg
    40621 ccgagcaatc cggcattatg caaaacacca tccagacgcg gataattaac ggcaatgcgc
    40681 tgtgccagtt gttggcaatt ttcggacgtg caggtcagca aatcgagaat aaaccactgt
    40741 ggctgacgcc cagtttcttc gtttatgtgg ctggctacct gacgtaattt ttcttcatta
    40801 cggcccaaca gaatcactgt cgcaccatag cgtgcatacg tcatcgcggc ttcacgacca
    40861 ataccatcgc tggctcccgt caccaggata atgcgatcat tgagtaaatc ttgttttggc
    40921 tggtaatgca tggctactcc tcaacgacgt tgtctgtcgc gctgccagtg tactttatga
    40981 ctttggggct ttatgcctga aacagaaggg catttcaatc agcctggggc gataacaccg
    41041 caaaatggat actttgtcat actttcgctg caataacatc tctgcgagac ggcttaacat
    41101 gcctgttgta aac
//
LOCUS       DBBGLQ010000040        38686 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.040, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000040 DBBGLQ010000000
VERSION     DBBGLQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.040"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            129..893
                     /gene="yfeN"
                     /locus_tag="ACIYSB_002703"
     CDS             129..893
                     /gene="yfeN"
                     /locus_tag="ACIYSB_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HGC5429724.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(932..1858)
                     /gene="yfeR"
                     /locus_tag="ACIYSB_002704"
     CDS             complement(932..1858)
                     /gene="yfeR"
                     /locus_tag="ACIYSB_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5429725.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1948..2946
                     /gene="yfeH"
                     /locus_tag="ACIYSB_002705"
     CDS             1948..2946
                     /gene="yfeH"
                     /locus_tag="ACIYSB_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGC5429726.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2943..3161)
                     /gene="ypeB"
                     /locus_tag="ACIYSB_002706"
     CDS             complement(2943..3161)
                     /gene="ypeB"
                     /locus_tag="ACIYSB_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGC5429727.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(3163..5178)
                     /gene="ligA"
                     /locus_tag="ACIYSB_002707"
     CDS             complement(3163..5178)
                     /gene="ligA"
                     /locus_tag="ACIYSB_002707"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGC5429728.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(5249..6235)
                     /gene="zipA"
                     /locus_tag="ACIYSB_002708"
     CDS             complement(5249..6235)
                     /gene="zipA"
                     /locus_tag="ACIYSB_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGC5429729.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPHPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6465..7226
                     /gene="cysZ"
                     /locus_tag="ACIYSB_002709"
     CDS             6465..7226
                     /gene="cysZ"
                     /locus_tag="ACIYSB_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGC5429730.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7411..8382
                     /gene="cysK"
                     /locus_tag="ACIYSB_002710"
     CDS             7411..8382
                     /gene="cysK"
                     /locus_tag="ACIYSB_002710"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGC5429731.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8766..9023
                     /gene="ptsH"
                     /locus_tag="ACIYSB_002711"
     CDS             8766..9023
                     /gene="ptsH"
                     /locus_tag="ACIYSB_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGC5429732.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            9068..10795
                     /gene="ptsI"
                     /locus_tag="ACIYSB_002712"
     CDS             9068..10795
                     /gene="ptsI"
                     /locus_tag="ACIYSB_002712"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGC5429733.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRREILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10836..11345
                     /gene="crr"
                     /locus_tag="ACIYSB_002713"
     CDS             10836..11345
                     /gene="crr"
                     /locus_tag="ACIYSB_002713"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGC5429734.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11388..12239)
                     /gene="pdxK"
                     /locus_tag="ACIYSB_002714"
                     /gene_synonym="thiJ"
     CDS             complement(11388..12239)
                     /gene="pdxK"
                     /locus_tag="ACIYSB_002714"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGC5429735.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVVTSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12344..12718
                     /gene="yfeK"
                     /locus_tag="ACIYSB_002715"
     CDS             12344..12718
                     /gene="yfeK"
                     /locus_tag="ACIYSB_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HGC5429736.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCYEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12751..13485
                     /gene="yfeS"
                     /locus_tag="ACIYSB_002716"
     CDS             12751..13485
                     /gene="yfeS"
                     /locus_tag="ACIYSB_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HGC5429737.1"
                     /translation="MKKRFIYHDEKSNKFWWIDYEGDSLAVNYGKVGSIGKFQTKEFD
                     NEEQCLKEASKLIAAKMKKGYQEDPKFNFMDRYYFDDEEIGLHVKTSHPNFQCHFTDP
                     LYMCCWDEESPFGSDEGADALNVLENSLRKEPDLDCADFPQMLIETMWGMKYIAMDSI
                     LEEDVRAQLLVDEMSTIQSNMITYATAFGQIKVMGKISHKLKKMGLNALARHQLTAKI
                     LQWGDGQDSPILQKMIDDLTAFPHEN"
     gene            complement(13674..14585)
                     /gene="cysM"
                     /locus_tag="ACIYSB_002717"
     CDS             complement(13674..14585)
                     /gene="cysM"
                     /locus_tag="ACIYSB_002717"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGC5429738.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPDAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14719..15816)
                     /gene="cysA"
                     /locus_tag="ACIYSB_002718"
     CDS             complement(14719..15816)
                     /gene="cysA"
                     /locus_tag="ACIYSB_002718"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGC5429739.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15806..16681)
                     /gene="cysW"
                     /locus_tag="ACIYSB_002719"
     CDS             complement(15806..16681)
                     /gene="cysW"
                     /locus_tag="ACIYSB_002719"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGC5429740.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16681..17514)
                     /gene="cysT"
                     /locus_tag="ACIYSB_002720"
     CDS             complement(16681..17514)
                     /gene="cysT"
                     /locus_tag="ACIYSB_002720"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGC5429741.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17514..18530)
                     /gene="cysP"
                     /locus_tag="ACIYSB_002721"
     CDS             complement(17514..18530)
                     /gene="cysP"
                     /locus_tag="ACIYSB_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGC5429742.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(18834..19625)
                     /gene="ucpA"
                     /locus_tag="ACIYSB_002722"
     CDS             complement(18834..19625)
                     /gene="ucpA"
                     /locus_tag="ACIYSB_002722"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGC5429743.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19754..20611)
                     /gene="murR"
                     /locus_tag="ACIYSB_002723"
     CDS             complement(19754..20611)
                     /gene="murR"
                     /locus_tag="ACIYSB_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGC5429744.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALLDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            20775..21671
                     /gene="murQ"
                     /locus_tag="ACIYSB_002724"
     CDS             20775..21671
                     /gene="murQ"
                     /locus_tag="ACIYSB_002724"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGC5429745.1"
                     /translation="MQFEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21675..23099
                     /gene="murP"
                     /locus_tag="ACIYSB_002725"
     CDS             21675..23099
                     /gene="murP"
                     /locus_tag="ACIYSB_002725"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGC5429746.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            23104..24408
                     /gene="pbp4b"
                     /locus_tag="ACIYSB_002726"
     CDS             23104..24408
                     /gene="pbp4b"
                     /locus_tag="ACIYSB_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGC5429747.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDVGYPSVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMTIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(24648..25547)
                     /gene="yfeX"
                     /locus_tag="ACIYSB_002727"
     CDS             complement(24648..25547)
                     /gene="yfeX"
                     /locus_tag="ACIYSB_002727"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGC5429748.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(25643..26218)
                     /gene="yfeY"
                     /locus_tag="ACIYSB_002728"
     CDS             complement(25643..26218)
                     /gene="yfeY"
                     /locus_tag="ACIYSB_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGC5429749.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(26279..26728)
                     /gene="yfeZ"
                     /locus_tag="ACIYSB_002729"
     CDS             complement(26279..26728)
                     /gene="yfeZ"
                     /locus_tag="ACIYSB_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGC5429750.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(26715..27140)
                     /gene="ypeA"
                     /locus_tag="ACIYSB_002730"
     CDS             complement(26715..27140)
                     /gene="ypeA"
                     /locus_tag="ACIYSB_002730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGC5429751.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGDVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            27354..28223
                     /gene="amiA"
                     /locus_tag="ACIYSB_002731"
     CDS             27354..28223
                     /gene="amiA"
                     /locus_tag="ACIYSB_002731"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGC5429752.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            28227..29126
                     /gene="hemF"
                     /locus_tag="ACIYSB_002732"
     CDS             28227..29126
                     /gene="hemF"
                     /locus_tag="ACIYSB_002732"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGC5429753.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGADPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(29132..30184)
                     /gene="eutR"
                     /locus_tag="ACIYSB_002733"
     CDS             complement(29132..30184)
                     /gene="eutR"
                     /locus_tag="ACIYSB_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGC5429754.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRNQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLF
                     SEKPSLTLHQRMREWG"
     gene            complement(30230..30730)
                     /gene="eutK"
                     /locus_tag="ACIYSB_002734"
     CDS             complement(30230..30730)
                     /gene="eutK"
                     /locus_tag="ACIYSB_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGC5429755.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(30743..31402)
                     /gene="eutL"
                     /locus_tag="ACIYSB_002735"
     CDS             complement(30743..31402)
                     /gene="eutL"
                     /locus_tag="ACIYSB_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGC5429756.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MIAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLATYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(31412..32299)
                     /gene="eutC"
                     /locus_tag="ACIYSB_002736"
     CDS             complement(31412..32299)
                     /gene="eutC"
                     /locus_tag="ACIYSB_002736"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGC5429757.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VNLAKRMLEQKASGINMTR"
     gene            complement(32320..33681)
                     /gene="eutB"
                     /locus_tag="ACIYSB_002737"
     CDS             complement(32320..33681)
                     /gene="eutB"
                     /locus_tag="ACIYSB_002737"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGC5429758.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(33693..35096)
                     /gene="eutA"
                     /locus_tag="ACIYSB_002738"
     CDS             complement(33693..35096)
                     /gene="eutA"
                     /locus_tag="ACIYSB_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGC5429759.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSRLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYHAAGIEPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSHGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVGAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(35093..36319)
                     /gene="eutH"
                     /locus_tag="ACIYSB_002739"
     CDS             complement(35093..36319)
                     /gene="eutH"
                     /locus_tag="ACIYSB_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGC5429760.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(36536..37723)
                     /gene="eutG"
                     /locus_tag="ACIYSB_002740"
     CDS             complement(36536..37723)
                     /gene="eutG"
                     /locus_tag="ACIYSB_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGC5429761.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLTVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVTLLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(37713..38549)
                     /gene="eutJ"
                     /locus_tag="ACIYSB_002741"
     CDS             complement(37713..38549)
                     /gene="eutJ"
                     /locus_tag="ACIYSB_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGC5429762.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(38560..>38686)
                     /locus_tag="ACIYSB_002742"
     CDS             complement(38560..>38686)
                     /locus_tag="ACIYSB_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGC5429763.1"
                     /translation="GLGGEGWTTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
BASE COUNT         9122 a        10635 c         9868 g         9061 t
ORIGIN      
        1 tttctctatt aatccacggt aaaaactgta ttggcgatat taattccttt gcattaacaa
       61 tatgtcgttt ttagcaaacc actttattaa gcaatctact aataacgatc ggcataggga
      121 attatgctat gaaaaaacat cttttaactc tgacactttc ctctatatta gcgatacctg
      181 ttgtatctca tgcagaattt aaaggcggtt ttgcagatat cggcgtgcat tatctggact
      241 ggaccagccg gactaccgaa aaatcgtcga ccaaatcaca caaagatgat tttggttatc
      301 ttgaatttga aggtggcgct aactttagtt ggggagaaat gtacgggttc ttcgactggg
      361 aaaacttcta taacggtcgc cataataaac caggtagcga gcagcgttat acctttaaaa
      421 ataccaaccg tatttacctg ggtgacaccg gatttaatct ctacttgcat gcgtatggca
      481 cctacggttc tgcgaatcgc gtgaatttcc acgacgatat gttcctgtac ggtatcggct
      541 acaatttcac cggcagcggt tggtggttca aaccgttctt tgctaagcgt tatacagatc
      601 aaacctatta cactggcgac aacggctatg tcgccggctg ggttgcaggt tacaacttta
      661 tgctgggcag cgagaaattc actcttacca actggaacga gtacgagttt gaccgtgacg
      721 ctacctatgc agcgggtaat ggcggtaaag aagggctgaa tggcgcagtt gcactgtggt
      781 ggaatgcaac atcacacatt actacgggga ttcagtatcg atatgcggat gacaaactgg
      841 gcgaagattt ttaccaggat gcgataatct actccatcaa atttaacttc tgattgatta
      901 ctgccggggc tcccggcagt atgaattcac tctatatctg atacaacgga tccccttccc
      961 gcccagccat tagcgcattg ccctggtctc gtaccacgtc ccataaagct tcagcggcgg
     1021 tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaaca accggcgtaa
     1081 tgcgctttac caccagtggg ctaccttcag gcaatggtag cgccagtgcg ggaagaatac
     1141 tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca atctcctgca
     1201 caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt cggctgcctg
     1261 acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt acataatctt
     1321 ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa agaatcgctt
     1381 cacattgcag atcgcccact gggcctggat cgatgacgat gccaaaatcg acatctccct
     1441 ggcgaatact ttccattacc cactgctgcg gacgatcgtg caggacaaac tgaatatctg
     1501 gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa atggtctggc
     1561 tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta tcgcgcaacg
     1621 tgctgttcag ttcatccagc agtcgctcaa gacgcaaagc cagctgctgc cctgcatctg
     1681 taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga ttttccagtt
     1741 ccttcacact gtgactcact gccgactggc tcaggccgat acgctctcct gcacgactaa
     1801 aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa taattcatct
     1861 gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga agaagcacaa
     1921 tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga tcctttcacc
     1981 ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg cgatttcgtc
     2041 cccttctttg aaaatctgac caccgcagct attgccctgc tgttctttat gcacggcgcg
     2101 aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca tttgtgggta
     2161 atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg gtggaaaccg
     2221 gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct cccggctacc
     2281 gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc agcggtttgt
     2341 tctgcgtcgg catccagcct gctggggatt ttcctttcac cattgctggt tggtctggtg
     2401 atgaatgttc acggtgcagg gggcagcctt gagcaggtcg gtaaaattat gctgcaactg
     2461 ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg ggtgtcgcgc
     2521 aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt ttatacagcg
     2581 ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc attgctgttt
     2641 atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt ctttatggca
     2701 cgccgactga gcttcaataa ggcagatgaa attactatcg tcttttgtgg ttcgaaaaag
     2761 agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt gatcggtatg
     2821 atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc ggtgctggcg
     2881 cgtcgataca aacgccagac cgaacagtta caggcgcagc aggaaagcag cgccgataaa
     2941 gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt tttaatcagc
     3001 agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt gcgggcaaac
     3061 cacagcagat actcctccgg caggtcgatt aagcgacgcc ctttgtattt gccaaacggc
     3121 attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac ccagcaaacg
     3181 cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg ccagtttaga
     3241 tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc ccgcgacttt
     3301 cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc gacatctggc ttaagctgcc
     3361 cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt tgataacgat
     3421 cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc ggttgctttc
     3481 ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag gcaccttttg
     3541 cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg ccgccagacc
     3601 tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc gagcaaaggt
     3661 ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccgattttg gccccatacg
     3721 ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tctgccggag tatggacata
     3781 ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat ccatcgcacg
     3841 gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca ggccaccggt
     3901 acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata ccggacaatg
     3961 cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga cgttaaccac
     4021 ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc gtaaaccaag
     4081 acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc ctgcaacatg
     4141 gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct gaaactcgac
     4201 gtcacgcaca aaggtcatct gctcctgcgc cgggaattta aacgctaccg cccagcgcgg
     4261 ggcacgcgcg acaaagccaa gctgctcctg ctgtgccagt gagttgacct taatcaccac
     4321 gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat agaacgccag
     4381 cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaacc cccacttttt
     4441 aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca gaacaccaac
     4501 gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa gctgacgcag
     4561 tgaaccagct gccgcattac gtgggttagc aaacactttc ccgcccgtgc gtcgcgcatc
     4621 ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca cttccagacg
     4681 cgccgggata ttctctccgt gcagcttcag cggaatggcg cgaatagtac gcacattaga
     4741 cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg gcactgacta aaacgccatt
     4801 ttcatacaga atactgacgg caagaccatc cagcttcagc tcacagcacc aggtgacttt
     4861 ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc tttcttcatc
     4921 aaaaacgtta tccagtgaca gcattggtac ttcatggcgt atctggctga aagccgccag
     4981 cggcgcagcg cctacacgtt gagtaggcga atcaggcgta atcagttctg gatgtttggt
     5041 ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa tttccggcgc
     5101 atccatcaca tgataaagat attcatgatg gcgaagcgtc gttcgcagtt ctgtcagttg
     5161 ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa gcgggggttc
     5221 gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact tcgcggatga
     5281 tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg tcaagcacga
     5341 caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg aagttctgca
     5401 gctcgtcacc gtaagacggt acctgcataa agatagtgac acccggagta gtgaaatcct
     5461 tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctgaat aacgccgggc
     5521 cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa atgaagcccg
     5581 cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga tgcgccgcga
     5641 cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga gcaggttccg
     5701 ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc tgttgtgccg
     5761 gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac ctgtggcgaa actggctgct
     5821 gcaacggctg ttcaggctgc ggctgatagg caggctgctg caccggctgc gctggatgcg
     5881 gagcatgttg cggcggtacc tgcgcttcag gcggctgctg gaccggttga cgcggctgcg
     5941 cagacgcata aggcggttgg tactggtgtt gcggcgacgg acgagcagcc tcatgctcct
     6001 gagcgttagc cggggcatga ttcacgcggt gaacacgaac ctcaccaacg ccctcatcat
     6061 cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga cttcattcgt tttaatggcc
     6121 gatcgcggaa catagaagat cgttctttac ggctggtcca gaaaccatgt accagtaaag
     6181 cgattatggc gatcgcgcca acaatgatta atatcagacg caaatcctgc atcattatat
     6241 tctctgttgt tctaacacct tgccaccacg gcaaacattt actcactaag agtatttgcc
     6301 gattacctca agtgcaagtg cactattaac tttcacagca caaagataga tgaaatcgtg
     6361 ctttttgctg ttttttcgaa catatcctaa ctgtccattg cgcaattacc cggtaaaata
     6421 cgcagaattt tcctgggatt ggtcaaaagg agctcatcct gattatggtt tcatcattca
     6481 catctgcccc acgcagcggt ttttactatt ttgcgcaagg ctggaagctc gtctcgcaac
     6541 ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa tattttgttg atggggggcg
     6601 cattctggtg gctctttaca cagctcgatg tctggatccc gactctcatg agttacgttc
     6661 cggactggct acaatggctg agttatctgt tgtggcctct ggctgtcatc tctgtgctgt
     6721 tagtgtttgg ctatttcttc tccacgattg ctaactggat tgccgctccg tttaacggtt
     6781 tattggctga acaactggaa gcacgattga ctggcgctac accgccagat accgggattt
     6841 tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga atggcaaaaa tttgcctggt
     6901 atctgccgcg cgcaattgta ttactaattc tttacttcat ccctggtatt gggcaaaccg
     6961 tcgcgccggt actgtggttc ctgtttagcg cctggatgtt agccatccag tattgcgatt
     7021 accccttcga taaccacaaa gtgccgttta aagagatgcg caccgccctg cgcacacgca
     7081 aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt tacgatgatc ccgctgctta
     7141 atctgttcat catgcccgtt gccgtttgtg gcgcgacggc gatgtgggtc gattgctatc
     7201 gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta ttttgtaaac cgtttgtgtg
     7261 aaacaggggt ggcttatgcc gccccttatt ccatcttgca tgtcattatt tcccttctgt
     7321 atatagatat gctaaatcct tacttccgca tattctctga gcgggtatgc tacctgttgt
     7381 atcccaattt catacagtta aggacaggcc atgagtaaga tttttgaaga taactcgctg
     7441 actatcggtc acacgccgct ggttcgcctg aatcgcatcg gtaacggacg cattctggcg
     7501 aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc gtatcggtgc caacatgatt
     7561 tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg aactggttga accgaccagc
     7621 ggtaataccg ggattgcact ggcctatgta gctgccgctc gcggttacaa actcaccctg
     7681 accatgccag aaaccatgag tattgaacgc cgcaagctgc tgaaagcgtt aggtgcaaac
     7741 ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa tccaaaaagc agaagaaatt
     7801 gtcgccagca atccagagaa atacctgctg ctgcaacaat tcagcaatcc ggcaaaccct
     7861 gaaattcacg aaaagaccac cggtccggag atatgggaag ataccgacgg tcaggttgat
     7921 gtatttattg ctggcgttgg gactggcggt acgctgactg gcgtcagccg ctacattaaa
     7981 ggcaccaaag gcaagaccga tcttatctct gtcgccgttg agccaaccga ttctccagtt
     8041 atcgcccagg cgctggcagg tgaagagatt aaacctggcc cgcataaaat tcagggtatt
     8101 ggcgctggtt ttatcccggc taacctcgat ctcaagctgg tcgataaagt cattggcatc
     8161 accaatgaag aagcgatttc taccgcgcgt cgtctgatgg aagaagaagg tattcttgca
     8221 ggtatctctt ctggagcagc tgttgccgcg gcgttgaaac tacaagaaga tgaaagcttt
     8281 accaacaaga atattgtggt tattctacca tcatcgggtg agcgttattt aagcaccgca
     8341 ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt aatgccagct tgttaaaaat
     8401 gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg cttcaaactt tcgcccctcc
     8461 tggcattgat tcagcctgtc ggaactggta tttaaccaga ctaattattt tgatgcgcga
     8521 aattaatcgt tacaggaaaa gccaaagctg aatcgatttt atgatttggt tcaattcttc
     8581 ctttagcggc ataatgttta atgacgtacg aaacgtcagc ggtcaacacc cgccagcaat
     8641 ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa ctggcgctaa caatacaggc
     8701 taaagtcgaa ccgccaggct agactttagt tccacaacac taaacctata agttggggaa
     8761 atacaatgtt ccagcaagaa gttaccatta ccgctccgaa cggtctgcac acccgccctg
     8821 ctgcccagtt tgtaaaagaa gctaagggct tcacttctga aattactgtg acttccaacg
     8881 gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac tctgggcctg actcaaggta
     8941 ccgttgtgac tatctccgca gaaggcgaag acgagcagaa agcggttgaa catctggtta
     9001 aactgatggc ggaactcgag taatttcccg ggttctttta aaaatcagtc acaagtaagg
     9061 tagggttatg atttcaggca ttttagcatc cccgggtatc gctttcggta aagctctgct
     9121 tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt tctgccgacc aggttgatca
     9181 ggaagttgaa cgttttctga gcggtcgtgc caaggcatca gcccagctgg aaacgatcaa
     9241 aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc atctttgaag ggcatattat
     9301 gctgctcgaa gatgaggagc tggagcagga aatcatagcc ctgattaaag ataagcacat
     9361 gacagctgac gcagctgctc atgaagttat cgaaggtcag gcttctgccc tggaagagct
     9421 ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt gatatcggta agcgcctgct
     9481 gcgcaacatc ctgggcctga agattatcga cctgagcgcc attcaggatg aagtcattct
     9541 ggttgccgct gacctgacgc cgtccgaaac cgcacagctg aacctgaaga aggtgctggg
     9601 tttcatcacc gacgcgggtg gccgtacttc ccacacctct atcatggcgc gttctctgga
     9661 actacctgct atcgtgggta ccggtagcgt cacctctcag gtgaaaaatg acgactatct
     9721 gattctggat gccgtaaata atcaggttta cgtcaatcca accaacgaag ttattgataa
     9781 aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca gagcttgcta aactgaaaga
     9841 tctgccagct attacgctgg acggtcacca ggtagaagta tgcgctaaca ttggtacggt
     9901 tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc gttggtctgt atcgtactga
     9961 gttcctgttc atggaccgcg acgcactgcc cactgaagaa gaacagtttg ctgcttacaa
    10021 agcagtggct gaagcgtgtg gctcgcaagc ggttatcgtt cgtaccatgg acatcggcgg
    10081 cgacaaagag ctgccataca tgaacttccc gaaagaagag aacccgttcc tcggctggcg
    10141 cgctatccgt atcgcgatgg atcgtagaga gatcctgcgc gatcagctcc gcgctatcct
    10201 gcgtgcctcg gctttcggta aattgcgcat tatgttcccg atgatcatct ctgttgaaga
    10261 agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag gaactgcgcg acgaaggtaa
    10321 agcgtttgac gagtcaattg aaatcggcgt aatggtggaa acaccggctg ccgcaacaat
    10381 tgcacgtcat ttagccaaag aagttgattt ctttagtatc ggcaccaatg atttaacgca
    10441 gtacactctg gcagttgacc gtggtaatga tatgatttca cacctttacc agccaatgtc
    10501 accgtccgtg ctgaacttga tcaagcaagt tattgatgct tctcatgctg aaggcaaatg
    10561 gactggcatg tgtggtgagc ttgctggcga tgaacgtgct acacttctgt tgctggggat
    10621 gggtctggac gaattctcta tgagcgccat ttctatcccg cgcattaaga agattatccg
    10681 taacacgaac ttcgaagatg cgaaggtgtt agcagagcag gctcttgctc aaccgacaac
    10741 ggacgagtta atgacgctgg ttaacaagtt cattgaagaa aaaacaatct gctaatccac
    10801 gagatgcggc ccaatttact gcttaggaga agatcatggg tttgttcgat aaactgaaat
    10861 ctctggtttc cgacgacaag aaggataccg gaactattga gatcattgct ccgctctctg
    10921 gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt tgcggaaaaa atcgttggtg
    10981 atggtattgc tatcaaacca acgggtaaca aaatggtcgc gccagtagac ggcaccattg
    11041 gtaaaatctt tgaaaccaac cacgcattct ctatcgaatc tgatagcggc gttgaactgt
    11101 tcgtccactt cggtatcgac accgttgaac tgaaaggcga aggcttcaag cgtattgctg
    11161 aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga atttgatctg ccgctgctgg
    11221 aagagaaagc caagtctacc ctgactccgg ttgttatctc caacatggac gaaatcaaag
    11281 aactgatcaa actgtccggt agcgtaaccg tgggtgaaac cccggttatc cgcatcaaga
    11341 agtaattctt gccgcagtga aaaatggcgc ccatcggcgc cattttttta tgcttccgcc
    11401 agcggcggca aaatcaattc atcgctctca tgctgctggg tgtagcgcat cacttccagt
    11461 acgcgcaacc ccgctcggtg cactgcatcg gttaacgcct tccctttcag caagccactg
    11521 atgagctgag cacaaaacag gtcgccagtc cctttcaggt cggtttttac ccgtgaatgg
    11581 gaaatgacat tcacgctgtc ggcagtgacc accacaacct gcatctcctg attttcttca
    11641 ttaccggagg cgctggtaac caccacccat tttaatgtgt ctgaaagcag actttttgcg
    11701 gcagcaatgg cactgtcgag atcgcggcaa tttttaccgg tcaggatttc caactcaaag
    11761 atattggggg taattccctg cgccagcggc agtaaatatt gtcgatacgc ttcgggaagg
    11821 tcaggtttga cataaattcc gctatcaata tcgccaatca ccggatcgac catgatcaat
    11881 aggtcaggat ggtctttgcg tagcgcagtc agccactcgg caaggatttt gatttgcgat
    11941 gccgttccca tatagcccgt ggttacagca cgaagttggc gcagcgcatc acgctcctga
    12001 agcgcacgca aatagccgct aaaccattcg tccggaatcg caccaccgta gaaagtgtca
    12061 taatgcggcg tattgctcag caataccgtc ggcacggcaa agacattcag gccgttctgt
    12121 ttgatagcag gcacggcaat gctgttgccc acgctgccgt aaaccacctg cgactgcacg
    12181 gcgacgatat ccgcctgcag tgccctactc ttatcgttaa acaacaacaa actactcatt
    12241 taattttttc tccttgccga tgatcctcat cgtaatccaa ccgaaacttt acctgattct
    12301 ggcagtcaaa tcggctatca caaaacaagg ataaggtaat tcaatgaaga aaatcatttg
    12361 tctggtcatt acactactaa tgacactccc cgtttacgcg aagttaactg cccatgaaga
    12421 agcccgcatc aacgccatgc tggagggatt agcacagaaa aaggatttga tatttgtgcg
    12481 caacggtgat gaacatacct gctatgaagc ggtttctcat ctgcgtctga agctcggcaa
    12541 tacccgtaac cgcattgaca ctgccgagca gtttattgat aaggttgctt cgtcgtcatc
    12601 gattactggg aagccgtata ttgtgaagat ccccggtaag agcgatgaga acgcacagcc
    12661 ttttttacat gcgttaattg cgcagacgga taaaacggtg cctgcggaag gaaattaatc
    12721 cgctttggga aggcatttac aggaggtaac atgaaaaaac gctttattta tcacgatgaa
    12781 aaatcgaata aattttggtg gatagattac gaaggggata gtttagctgt caactatggc
    12841 aaggtaggta gtattggtaa attccagaca aaagagttcg ataatgaaga acagtgtctg
    12901 aaagaagcca gtaaattgat tgccgcaaaa atgaagaaag gctatcaaga agatccaaag
    12961 tttaacttca tggatcgcta ctattttgat gatgaagaaa ttgggttaca tgttaaaacg
    13021 tcacacccaa acttccagtg ccattttact gatccacttt atatgtgttg ctgggatgaa
    13081 gaatctcctt ttggcagcga tgaaggtgct gatgctctaa acgttcttga aaatagcctc
    13141 cgtaaagagc cggatctgga ctgtgctgat ttccctcaaa tgttaattga aactatgtgg
    13201 ggtatgaaat acatcgctat ggacagtatt cttgaagagg atgttcgtgc gcaattacta
    13261 gtcgatgaaa tgagcactat ccagagcaat atgattacct acgcaactgc attcggtcag
    13321 attaaagtca tgggtaaaat ctcccataaa cttaaaaaga tgggactcaa tgcactagcg
    13381 cgtcatcagc ttaccgcaaa aattcttcaa tggggtgacg gtcaggactc accaatactt
    13441 caaaaaatga ttgatgacct tacggcgttt cctcacgaaa attaaatact gcatttgtcg
    13501 gcagcaacaa ctgttaaaaa agtgcgcttt gtttatgccg gatgcggcgt aaacgcctta
    13561 tccggcctac aaaatcgtgc taattcaata tattgcagaa accttgtagg cctgataagc
    13621 gtagcgcatc aggcagtttt gcgtttgtca tcagtctccg atgctattaa tccttaaatc
    13681 cccgccccct ggctaaaatg ctcttcccca aacaccccgg tagaaaggta gcgatcgcca
    13741 cgatcgcaga tgatcgccac caccaccgcg tcagggttag cttttgccac ccgcagtgct
    13801 ccggcaaccg cgccgccgga gctgacgcca cagaatattc cttcccgcac cgccagttcg
    13861 cgcatggtgt tttccgcatc gcgctgatga atatccagca cctcatccac cagagaagcg
    13921 ttgaaaatcc ccggcagata ttccgtaggc cagcggcgaa tgccgggaat gctgctgccc
    13981 tcttccggtt gcaggccgac aatggtcacc ggtttggatt gttcgcgcat aaagcgtgag
    14041 acgccggtga tagtgccggt cgtccccatg ctggagacaa aatgagtgat gcgcccgccg
    14101 gtttgctgcc agatttccgg cccagtggtg gtgtaatgcg cataagggtt atcgggatta
    14161 ttgaactgat cgagcagctt tccttcgcca cgattcgcca tctccagcgc cagatcgcgc
    14221 gcaccttcca tgccctgctc tttggtgaca agaatcagtt ccgcaccata agcacgcatc
    14281 gccgcacggc gttcctggct catgttgtcg ggcatcagca atttcatgcg atagcctttc
    14341 agcgcggcaa tcattgccag cgcaatgccg gtgttaccac tggtggcttc gattaagaca
    14401 tcacccggtt taatttcccc gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga
    14461 tctttcaccg aacctgccgg gttattgcct tccagtttta accacacttc actgccgtta
    14521 tccggcccca ttcgctgcaa cttcaccaga ggcgtattgc ctattgtttg ttctaatgta
    14581 ctcacgatct ctatccatac gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggt
    14641 ctacaggtta caaaccttgc cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt
    14701 taacattcac tcaacctatc aggcgctttg tgcgagagca agttcctcat cgcgggtttc
    14761 gatacgctcg tcgccgttat acagccgcgc atgttgcaga ccaacgaata aacgctcgcc
    14821 acgctgcggg gcatcgtcgc catgcatcac gaccgtcagc ggttcgttgt accaccccag
    14881 cggctgcacc actaattggg tgtagtgacc tttcgggctg gcttccagta cctgtaccgg
    14941 cagcggcgaa tcgaggctgg tacggcggct gatatccact tcccaagggc gcaggaagag
    15001 atccaccggc ccctgatacg caggtgtgta gcccagcggc cagcgatgcg cgccaacatg
    15061 gaactgcccg ccgcgaatgg ttccctgcag gcggttcact tcgcccataa attcgagcac
    15121 aaaacgggtc gccggttcgc gccatacctg atccggcgcg tcagcctgtt caatattgcc
    15181 ctggctcatc acaactacac gatcagctac ttcggtcgct tcttcctgat cgtgggtcac
    15241 aaaaacgctg gtgaatttta gttcttcatg gagttgacgc agccagcgac gcagctcttt
    15301 acgcacctgc gcatccagcg cgccaaacgg ttcatcaagc agcagaattt gcggttccac
    15361 agccagcgcg cgcgccagcg ccacgcgctg tttctggccg ccggaaagct gcgccggata
    15421 acgatccgcc agatgggcaa gctggaccat ttccagcaat tttgtcactt tcgctttgat
    15481 ggctgcggca ttcgggcgct cgcgacgcgg cagcaccgtc aggccaaaag cgatattgtc
    15541 gaacaccgtc atatggcgga acagcgcgta atgctggaac acgaaaccga ctttacgatc
    15601 acgtgcgtgc aggcggctca cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg
    15661 ctccagcccg gcgataatgc gcagcagcgt ggttttcccg gaaccggacg gccccagcaa
    15721 cgcgaccatc tgacctgaag gaatatccag tgagatatcg ttcagcacct gggtgcgacc
    15781 aaacgacttc ttaatattgg caatctcaat gctcatgatg ttcctcctgc tgtgcgcgtt
    15841 tttcctgatt ctccaggcgc cactgcaaca tactttttaa aaacagggtg ataatcgcca
    15901 tcagcgttaa cagcgccgca gcggtaaagg agccgacggt gttgtagtcc tgctccagca
    15961 attcaatctg taacggcagc gacagggttt cgccgcgaat cgagccggaa accaccgaca
    16021 ccgcgccaaa ctcgccaatt gcgcgggcgt tggtcaacac cacgccataa agcagcgccc
    16081 agcggatgtt cggtaatgtg acgcgacgga acatctgcca gccggacgcg ccaagcaaaa
    16141 tcgccgcttc gtcttcctgg ctgccctggc ttaacatcac cggcaccagt tcgcgcacca
    16201 caaacggaca cgtcacgaag atggtgacca gcaccattcc cggccaggag aacataattt
    16261 gcaggttatg ctcgtcgagc caaccgccga gcgggccgtt agagccgtag aacagcaaat
    16321 acaccagacc ggcaaccacc ggcgatacgg caaacggaat gtccagtagc gtcagcagta
    16381 actggcgtcc agggaagtta aagcgcgtca ccagccaggc cagcagaatg ccgaacacca
    16441 ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt
    16501 ccggatcggc cagattctgt aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga
    16561 tgtaaatcat cggcaccagc aggatgaacg ccgaaaccag catcccgatg ccaatcagaa
    16621 accatttgcc ccagttaatc gggcgcgcgt cataacgctt caattgggta acttccgcca
    16681 ttaatgacct accacacgcc gaccaaagcg actttgcaga gtgttaattg agaacagcag
    16741 cagcagagat gccgcgagga tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc
    16801 ctgtaagcgc acaaaaatca tcagcgacgt cacttccgtc ttccacgcga tatttccggc
    16861 gataaaaatc accgcgccaa attcaccaag actacgggta aacgacagcg ccacgcccgc
    16921 caccagcgcc ggagaaagct ccggcagcac cactttgcag aaactctgcc agcgcgttgc
    16981 accaagcgtt tccgccgctt cttcatattc cgggcctaac tcttccagca ccggctgcac
    17041 ggtacgcacc acaaacggaa tgctggtaaa ggccatagcc accgcaatcc ccagccatgt
    17101 ataggtgact ttgatatcaa acttcgccag ccattcaccg taaaaaccgt ttacggaaaa
    17161 gagcgaggcc agcgttaaac cggcgacagc cgttggcagc gcaaagggta aatccatcag
    17221 cgcatcaagc agcgtgcggc ctgggaagcg atagcgggtt aggatccacg ccatcagcag
    17281 accgaaaacg ccgttaaaaa tcgatgccac aaacgccgac agcagcgtta ctttgtaggc
    17341 cgcgaccacc tgcgggttgg tgatcacctc ccagtactgc gcccagctca tctgggccag
    17401 ttgcatcacc agcgcggaga gcggcagcag caaaatcagg cacacaaaca gcagactggt
    17461 gccgaggctt aaggtaaagc ccggcagcac gcgtctggag gagacagcaa acatcagtta
    17521 cgccccgccg ctaacagctt gtctaactcg ccgccgctgg tgaagtgggt tttcatcact
    17581 tccggccagg agccaaattt gtcttccacg cggaacagct cggtctgcgg gaatttgtct
    17641 ttcagtttgt ccatcacctc cgggttattc acgcggtagt aatagtcggt gatgatggtt
    17701 tgcgcctgcg ggctatagag ccagttcaga taggctttgg cggctttttc cgtaccgttg
    17761 gcctgcacgt ttttatcaac ccacgccacc gggaattccg ccagaatgtt ggttttcgga
    17821 atcaccactt caaagccctg cgcttcatac tgtttacgga tgttgttcac ttccgattcg
    17881 aagctaatca gcacatcgcc caggccgcgc tcggcaaaag tggtggtcgc gccacgaccg
    17941 ccagtatcga acacttcaac gtttttcagg aactgggtca taaactgttc ggttttgcct
    18001 ttgtcaccac cgtcagcttt atccgctgcg ccccatgccg ccagataggt ataacgcgcg
    18061 ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt cggagcgcac caggtcgttc
    18121 caatcgtgga tattcttcgg gttaccctta cgcaccagga agcccatggt ggagtagaac
    18181 ggcgagctat tattcggcag gcgcgactgc cagtcggccg ggatcagctt gcctttatcg
    18241 tgcaggattt gtacgtcggt cacctggtta taagtgacaa cgtcggcttt taagccctgt
    18301 aaaatcgcca gcgcctgttt tgatgacccg gcatgagatt gttttatcgt cagtttgtcg
    18361 ccgccgttat cttttgccca ttgttgctca aacggcggat tcagggcggc aaacagctcg
    18421 cgggagacgt cataagaact gttcagcagt tccgttgcct gtacatggcc cgccagcagc
    18481 agagaagcga ccagcgcgag tgagttcttt ttcagtaagt taacggccat tgcgcaccct
    18541 tataaattta atgactttct aatagccatc atatttataa cggacgtgaa aggagtaacg
    18601 gttttatata ccgtttggtg atttggaagt tgaaaaggga ataagaactt ggttcaacag
    18661 gcgtaattgt tgcagtcagt ttggacacga acagcgcgga gaaaccggag cgtacacata
    18721 gtacgtgagg atttcgagca ctgcccgggg ccaaaatgac aaataaaata gcctggtgaa
    18781 cttagttcaa gacgaaatcc tccccacaaa tgcagggagg aaacagaggt gaatcagata
    18841 ccgacgctaa ccgtctccgg cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt
    18901 aaatagctgg attcatccga tgcgaggaag gccgccagtt cgccgacttc cagcggatcg
    18961 gcgaggcgac gcatcgggat tgctttcgcc atttcagtca gcaccgactc tggatcttcc
    19021 gggttcgact ggcgggcaat gctttccgcc attggtgtgc gcacgtatcc cgggcaaatg
    19081 gcgttaacgc gaataccaga ctgcgcgtac tccaccgcca gcgattttgt caggccaaca
    19141 atcgccgctt tcgttaaggc gtacgccgtt tcgccaggat cggccaccat atcaccagtg
    19201 actgaagaca tcatcacaat gcgaccatct ttgcgggcaa tcatctccgg cagcaccgcc
    19261 ttcgtgacgt tccatacgcc tttaatattg atgtcaatat ggaaatcgcg atcgtcatcg
    19321 ctcatatcga ggaaactgcc cagacgacaa acgcctgcgt tattcaccag gatatcaatg
    19381 cgcccttctt tttccttcgc gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca
    19441 tcggcgacaa ccgccgtaca gcgatgacca cgaccacaca gttcgtccgc cagcttttcg
    19501 atctcagggg agatatccag caagattagg ttcgcgccat gacgtgcaaa agttctggca
    19561 attccttcgc caattccctg caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta
    19621 cccattttaa tgctctcctt gttaatacag agcgttacac tccattccct ctgaaaatac
    19681 aggataaaca aaatattgct gcggatcagc tcactaattc aggaaaatct caatccagtc
    19741 gctgctttat gcatcatttc aggcgttgtg ttagctcact gctgcgctga atcattttca
    19801 atgactccac atcattgagt tgcacaaggc caacaaacag taaatcggta acagaatttt
    19861 gcgccgtgcg ggtagacatc gacgagctac gccattcggt ttctccggaa acggtatcga
    19921 gggtgaaatg cgccagccgc cgcagcggag agtcagtcag agaagtaatg gcaatcaccg
    19981 ttgccccctg ctttcgtgcc gcttctgcgc acaagacaat ctctttttta ctgccgctgt
    20041 aagagatagc gatttgtaca tcgccttttt tcaacgcctg ggaaacggtg gcttgtacgt
    20101 gcgtgtcggc ttcgcaggcc acgcgatagc caattttcat cagtttgaac gacagatcgc
    20161 gccccaccag cgccgatccg cccaggcccg ttatctggat aaacggtgct ttactgatga
    20221 cctcaatgat tttttgcagc cgcgcgtaat cgagcaacgc gcaggtttgc tccagcgcca
    20281 gctctttttc gcgattcagt ttgcgcgcga tcacttccag cgaatcatca ctggtaatcg
    20341 aactgtgcag gtgcagtgcc gtggcattcg ttttctcccg gctggcactg tattcgccta
    20401 ttaacgccat acgcaattca gtaaatcctt gcgcgccgag tttttgggca aatttcacta
    20461 tgctcgactg gctaatgccg agctgtttcg ccatctgccg tgaagaaacg gattgcagct
    20521 cactcacgtt ggcctgcaga aagtcagcga ttttttgctc gttttcggtg aattcactcc
    20581 ccgcattgct tattttcgtc aggtacaaca tcttccactc tcctgggctg gcagcataaa
    20641 ttccgcttaa gttcttatga cgctcttcac actctgcgag tgtagactca atgattcctt
    20701 taagcctgtc aattcgggat atatgattcc atacatctgc aattatttga tcgtaaatag
    20761 taaggtcacc accgatgcaa tttgaaaaga tgattactga aggctcgaac accgcctcgg
    20821 ctgaaattga ccgcgtatcg acgctggaaa tgtgccggat tatcaacgat gaagataaaa
    20881 ccgtaccgct tgccgttgag cgcgtactgc cggatatcgc cgcggcgatc gatgttatcc
    20941 acgcccaggt aagcggcggc gggcgtctga tttacctcgg tgcgggaaca tccggtcgtc
    21001 tggggattct ggatgccagc gaatgtccgc ccacctacgg cgtgaaaccg ggtctggtgg
    21061 ttggtttgat tgctggcggc gaatatgcca ttcagcacgc ggtggaaggc gcggaagata
    21121 gccgggaagg cggtgttaat gatctgaaaa atattaattt aacggcacag gatgtggtgg
    21181 ttggcattgc tgccagcggt cgcacgccgt atgtgattgc cggactggaa tacgcacgcc
    21241 agctcggctg ccgcacagtg ggaatttcct gtaatccggg gagcgccgtt tcaaccaccg
    21301 ctgagtttgc cattacaccg attgtaggtg ccgaagttgt taccggttct tcgcggatga
    21361 aagcaggtac agcgcagaaa ctggtgctca atatgctttc caccgggctg atgattaaat
    21421 ccggcaaagt gttcggcaac ctgatggtcg atgtggtcgc caccaacgaa aaactgcatg
    21481 tgcgacaggt caatattgtt aaaaacgcca ccggatgtag cgcagagcaa gcggaagcgg
    21541 cgttaattgc ttgcgagcgc aactgtaaaa cggccattgt gatggtgctg aaaaatctcg
    21601 atgccgcaga agctaaaaaa cgcctggatc aacacggcgg ctttattcgt caggttttag
    21661 acaaggaata acccatggcc aaagagatca gcagtgaact tctgaacacc attcttaccc
    21721 gtgtcggcgg accgggaaat atcgccagtt gtggtaactg tatgacgcgc ctgcgtctgg
    21781 gtgtacatga cagttcactg gttgatccca atatcaaaac gctggaaggc gtgaagggcg
    21841 tcattttgac cagcgaccag gtgcaggtcg tttttgggcc tggtaaagcg catcgtgccg
    21901 caaaagcgat gagcgagctg ctgggagaag caccggtaca ggatgccgca gaaatcgccg
    21961 cccagaacaa acgtcagtta aaagccaaac aaacctccgg cgtgcaacaa tttctcgcca
    22021 aattcgccac catcttcacg ccgctgatcc ccggttttat tgccgccggt ctgctgctgg
    22081 ggatagcgac gttaattgcc acggtgatgc acgttccggc agatgctcag ggaacactac
    22141 ccgatgcgct gaattttatg aaggtgttca gcaaaggttt gttcactttc ctggtgattc
    22201 tggtgggcta taacgccgcc caggcatttg gcggcacggg cgtaaatggc gcaattatcg
    22261 ccgcgctctt tttgctcggt tataaccccg cagcgaccac cggttactac gccggttttc
    22321 acgatttctt tggtctgccc atcgatccgc gcggcaatat tatcggcgtg ctgattgccg
    22381 cgtgggcctg cgcccgcatt gaaggcatgg tgcgccgctt tatgccggac gatctcgaca
    22441 tgctgctgac ctcgttaatc accctgctga tcaccgctac gcttgcgtac ctgatcatta
    22501 tgccgctggg tggctggtta ttcgaaggta tgtcatggct gtttatgcac ctgaacagta
    22561 atccgttcgg ttgtgcggtt ttagccgggc tgttcctgat cgccgtggta tttggcgtgc
    22621 atcagggctt tattcctgtt tacctcgcgt taatggacag ccagggattc aacagcttat
    22681 ttcccatcct ttcaatggca ggcgcgggcc aggtgggcgc ggcgctggca ctctactggc
    22741 gggcgcaacc gcacagtgcg ctacgcagtc aggtacgcgg ggcgattatt cccggcctgc
    22801 tgggcgttgg tgaaccgctg atttacggtg tcaccctgcc ccgcatgaag ccgtttgtta
    22861 ccgcctgttt aggcggcgcg gcgggcggtt tgtttatcgg cctgatagcc tggtggggtc
    22921 tgccgatggg cttaaacagc gcctttgggc cgtctggtct ggtagcgctg ccgctgatga
    22981 cttccgcaca aggcatcctt ccggccatgg cggtttatgc tggcgggatt ctggtggcat
    23041 gggtttgcgg gtttattttc accacgctct ttggctgccg taacgtcaat ctggactgat
    23101 atcatgaaac ggacaatgct ctatctttct ctgctggctg ttagttgtag cgtcagcgcc
    23161 gcaaaatacc ctgttctgac agaaagctcg ccagagaaag cagggtttaa cgtcgaacgg
    23221 cttaaccaga tggatcgctg gattagccag caagttgatg tcggttatcc cagcgtaaac
    23281 ctgctgatca ttaaagataa tcagattgtg tatcgcaaag cctggggagc ggcgaaaaag
    23341 tacgatggca gcgtgttgat ggaacagcct gtcaaagcca ccaccgggac gctgtatgac
    23401 ctggcctcaa acaccaaaat gtacgccacg aacttcgccc tgcaaaagct gatgagcgaa
    23461 ggcaaactgc atcctgacga tcggattgcg aaatatattc cggggtttgc cgatagtcca
    23521 aatgacacca ttaaaggcaa aaacaccctg cggatttctg acctgctgca tcacagcggc
    23581 ggtttcccgg cagatccgca atacccgaat aaagcggtcg cgggcgcgtt atattcccag
    23641 gataaaggcc agacgctgga gatgatcaag cgcacgccgc tggaatatca gcccggcagc
    23701 aaacatatct acagcgatgt cgattatatg ctgcttggat ttatcgtcga gtcggttacc
    23761 ggtcagccgc ttgaccgcta tgttgaagag tcgatttatc gcccgctcgg cctgacacat
    23821 acggtattta acccgctgct gaaaggcttt aaaccgcaac aaattgccgc cacggaattg
    23881 aacggcaata cccgcgatgg cgtgatccat ttcccgaata tccgcacctc cactctctgg
    23941 ggtcaggtgc acgatgaaaa agccttttat tcgatgggcg gcgtttccgg gcacgcaggt
    24001 ttgttttcca ataccggcga tattgcggtg ttaatgcaaa cgatgctgaa cggcgggggc
    24061 tatggtgatg tgcagctgtt caatgcggaa acggtgaaga tgttcaccac cagctctaag
    24121 gaagatgcca cttttggcct cggctggcgc gtgaatggta atgccaccat gacgccgacg
    24181 tttggcacgc tggcaagccc gcaaacctac ggtcacactg gctggaccgg aacggtgacc
    24241 gttatcgatc cggtgaatca tatgacgatt gtgatgttaa gcaacaagcc acattcgccg
    24301 gttgccgatc cgcaaaagaa tcccaatatg ttcgaaagcg gtcagttgcc gattgcaact
    24361 tatggttggg tagttgatca ggtgtatgcg gcgttaaagc agaagtaata aaaaggccag
    24421 tcagtaatgt tactggctgg tctgagagtg ctgacaaacg caaaactgcc tgatgcgcta
    24481 cgcttatcag gcctacgtgg agcccacaat atattgaatt agcacgattt cgtaggccgg
    24541 ataaggcgtt cacgccgcat ccggcgtgta caacgcgcac ttggtcaaca atctgaggcc
    24601 agtcagtaat gttactgcct ggcctttaat caatgaatca gaaacgctta cagcgccatc
    24661 aacttgtcca gcgacggtgc gaaataatag ccgccggtta ccggtttggt gaaacgcaac
    24721 atcgcatcac gcttaccatc ggtatcgcca aacatgctca gcagttgctg ctcaatgtta
    24781 tgcagacgcg cgcagtaggc gcagaagtac agaccgtgag tgccactggc agtgccgtac
    24841 ggcaggctct ggcgaacaat cttcagccct ttgccatctt ctttcagatc aacgcgggtg
    24901 aggtgagagg tttccggacg ttcgtcgccg tcgatctctt cgttggcctc tttggtgcgc
    24961 ccgatcatca tctcctgatc gtgaacgctc atccggttga gctgcttcag gttgtgttcc
    25021 caacgctgga caaacacata gctgccgccc gcatccacgc cgtctttgat aaccgccact
    25081 tcgcgacgcg tctcttcacc cgccgggttt tccgtaccgt caacaaagcc gctcaggtca
    25141 cgctcttcaa cccaacggaa gccgtggatc tcttctttca cttcaatgca gtcaccaaag
    25201 gcttccatcg ccgcctgggc gacagagaag tttacgtcgt gacgcagaga aagaatgtgg
    25261 atcaacacat cgaactgggt cgtcggcgca aggcctttac cgtagcccgg aaaatctttc
    25321 agctcttctg ccccaacgcc gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc
    25381 accgcaccaa gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat
    25441 gttttactgg ccgcacgcag ggcgtcaact tcccctttca cgttggcttc gatccaaatc
    25501 gccgcgcggc aatgttctgg caaaatgcca ctctgaacct gagacattgt tcctcctgaa
    25561 aataataatg ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt tttttcgcgg
    25621 cggcggtttg ttcagacgca aattaacgcc gccagataat tttactgact ttccagtttt
    25681 tcagggtatc gtccgaaggc attaaccctt caggaccacg ccattccccg ctgaactggt
    25741 agctaatatg ttgactgcct tcggctttac attcgacggc acgattatca tcaccatcag
    25801 ctttttggca attgccaaat gctttgctgt aaaggtcgct aaacggtgta ccgattttaa
    25861 caccggtgtc agcaggaata tcgctatcca gcacgtcaat gcgactgatc gtgccttgat
    25921 cgccgttaat caccatcgcc acgttgtcgc ccttcatcac ttcaaaaaag cgcaccacgt
    25981 tgccgttcgc ggttttcatt ccgctgcgca ggcgataatc gccatcaagc gcatcggcaa
    26041 tggcttgttc ttgcagtggt gtggacgccg ttaattcacc cacgccctgc tcgctcactt
    26101 tggtggacga cccaaaccag ttccacgggt tagcggcaga ccagttaact gacgacatcg
    26161 tggaacagcc ggtcagcatc aatggcatag cgcataacat taaacgcagc gatttcatgt
    26221 cacttccttt ggttattcaa taacgttgct tggagtgcaa attcaccaaa aagtgccgtt
    26281 attcttttac ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc cagattaacg
    26341 ccacaatatc cgccactacc agcgccagac cgataccgct aacggattca ccgttcagcc
    26401 acagccacgg ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga aaatagagta
    26461 cacgccaggt gcgaggaaac gtagcccgcc gaccgctcaa caaaaacgcc agcaccgccg
    26521 gaatgccagg aatcagcccc agccagaaat tatcgtgatc gggataaaac agatttagca
    26581 gcgcagtacc ctgctcgcgc gacgcaccgg caatgacaaa cagcacccag gttcgcgcct
    26641 gaagcaatag cacaagccag aagagcaagg gtaaacgcag gcgaccgtgc gcatcataat
    26701 ggacaggatg aaactcagta ctcttcatct tcaatcaaac gcttacccag actcagcacg
    26761 tcggcgtgtt catatcccag gcgttcatac attccgagca ccatgtcgtt atcttccggc
    26821 acattgatct gaattttcgg gcagccacga gcaatcagct ttttctccag ccgattaagc
    26881 aacgcattgg caatcccacg cccacgaaac tctggatgca cgccaagata ataagcagac
    26941 ccgcgatgcc cgtcataacc gcccatcacc gttccgacca cgtcaccgtt tacctcagcg
    27001 accaaaaaca aactgacgtc atggttcatc ttacgctcga tgtccatttc cggatcgttc
    27061 cacggacgca gcaagtcgca acgctcccaa agggtgatga cctcttcgaa atcttcctgg
    27121 cgaaatacgc gtatctccat ggtattcgtt acctttttgc gggttaaaag gctgattatg
    27181 gcgtgaacgg tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt gacataatgg
    27241 ggagttgtca actattgaaa tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg
    27301 gattacgcga tacgatataa catctggaac tttattatta caactcaggc cgtatgagca
    27361 cttttaaacc actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc ggtttggctg
    27421 ccctgacgtt gtcaggaatg tcgcaagcca tcgccaaaga cgaactttta aaaaccagca
    27481 acggacacag caagccgaaa gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc
    27541 caggtcacgg cggaattgat accggagcga tcggacgcaa cggttcgaaa gaaaaacatg
    27601 tggtgctggc gattgctaaa aacgtccgtt ccattttgcg taatcatggg attgatgcgc
    27661 gtttaacgcg ttctggcgat acgtttatcc cactttacga tcgcgttgaa atcgcccata
    27721 aacatggcgc agatctgttt atgtcaattc atgccgatgg ctttaccaac ccgaaagctg
    27781 ccggtgcttc ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc
    27841 tgtctgaacg cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact gacaaggatc
    27901 acctattgca acaagtgctg tttgatctgg tgcaaacaga taccattaaa aatagtctga
    27961 cgctcggctc gcatattctg aagaagatta agccggtgca taaactgcac agccgcaaca
    28021 ccgaacaagc ggcatttgtg gtgttgaaat caccgtcggt tccttcggtg ctggtggaaa
    28081 cctcgtttat caccaacccg gaagaagaac ggctgttagg cacggcggcg tttcgtcaga
    28141 aaatcgccac agcgattgct gaaggcgtga tcagttattt ccactggttc gacaaccaga
    28201 aagcacattc gaaaaagcga taagttatga aacccgacgc acaccaggtt aaacagtttc
    28261 tgctcaacct tcaggatacg atttgtcagc agctgaccgc cgtcgatggc gcagaatttg
    28321 tcgaagatag ttggcagcgc gaagctggcg gcggcgggcg tagtcgggtg ttgcgtaatg
    28381 gtggtgtttt cgaacaggca ggcgtcaact tttcgcatgt ccacggtgag gcgatgcctg
    28441 cttccgccac cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt
    28501 cactggtagt gcatccgcat aacccgtatg ttcccaccag ccacgcgaat gtgcggtttt
    28561 ttattgccga aaaaccgggt gccgatcccg tctggtggtt tggcggtggc ttcgacttaa
    28621 ccccattcta tggttttgaa gaagatgcta ttcactggca tcgcaccgcc cgtgacctgt
    28681 gcctgccatt tggcgaagac gtttatcccc gttacaaaaa gtggtgcgac gaatacttct
    28741 acctcaaaca tcgcaacgaa cagcgcggta ttggcgggct gttctttgat gacctgaaca
    28801 cgccagattt cgaccgctgt tttgccttta tgcaggcggt aggcaaaggc tacaccgacg
    28861 cttatttacc aattgtcgag cgacggaaag cgatggccta cggcgagcgc gagcgcaatt
    28921 tccagttata tcgtcgcggt cgttatgtcg agttcaatct ggtctgggat cgcggcacgc
    28981 tgtttggcct gcaaactggc gggcgcaccg agtctatcct gatgtcaatg ccgccactgg
    29041 tacgctggga atatgattat cagccaaaag atggcagccc agaagcggcg ttaagtgagt
    29101 ttattaaggt cagggattgg gtgtaactcc ctcaccccca ctcccgcatc cgctgatgca
    29161 gcgtcagtga cggcttctcg gaaaacagct gctggtaatc cgtggcaaat tgccccagat
    29221 gccagaatcc ccactgcatg gcggcgtctt ttaccgtcat actttgcgac cacggactta
    29281 tcagttcgcg gcgtacggcg ttcaggcgaa tgcgtttcag ccacgcgttc gggccaatgc
    29341 ctaaaatagc gtgaaacgcg ttttgtagcg tgcggcggct gacatgcagt tgattacaca
    29401 aatccagcac cgtcaccggt tcggacatgt tttccagcac atattcacgg gcgcgggaaa
    29461 gcaatcgacg gtaactctga tgactgatgc tttccgccgt caccattggt tgcgcttctt
    29521 ccagcatggc ccccatcgcc attagcaaat tatcccccag cacttttcgc actgcaggct
    29581 gatggagatt ttccggattc tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca
    29641 gcgcggcttt atgctgctct ttcacttcca gcgccgactg gttacgcaac atatgtaata
    29701 cccgatccgg gttatgcaaa aagttagcct gccgggtgat gacatcttca gaaagcacca
    29761 cgcccaggat cgtgtaatca tccggcgtgc tcagttcaaa ttcagtgcca ccagggcggg
    29821 tggcgatttc cgcgcttccc agacattgcg aaccgataaa tccctgctca ccgcgcgtcg
    29881 ccggaatgcc aaaccagaac gagttcggcc agaccaggca cgactgacgc agcgccagac
    29941 cggtgtattc acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa
    30001 acttgcccgg atgcagctga tcgtaaatct gctgccaggc ggtaatcgtt aaagcatgtt
    30061 catagacatc cgttgtctgt cgttgatgaa cattatccac ttcgaccttc ggcgtgagct
    30121 tcaggttttc gggtaagggt tcatgataaa gatggtgcaa attggctgta cgggtctttt
    30181 tcatgatgtt aatgccgggt gttgtaggac acccggcacc tccgacaggt taatggggct
    30241 tgagacgata acgactactg cgtttacgta acgtcccggc agaaaagagc tgttcgagcg
    30301 catttctggc tttttccagc ggccagccaa agtgggcagc cacttctcct gccgtcattc
    30361 cctgacgtac tgatgttaac agcgccaaca actcgtcagc agattccgcg acgataactg
    30421 gcgcgtcggg ttcccttacg ggttgcttcg gctggcggtt aaagccagta accagccact
    30481 gggtgtcatc gtctggccga ccgatctcct tgcggctgat gacacgcccg gtacgcatcg
    30541 cggcagcaca acctgcgtcc agcgccgcac gacacgccgc cagatcgcct tccaccacca
    30601 gcgttaagcg accagggtca agcacttcgt gactgagcag acgaacgtta gctgctttga
    30661 gcatggcatc cgctgcatct atcgcggcga ccatgccgtc cacttccagc aatcccagtg
    30721 cattgatcat cggcaacctc cgttacgcac gctggattgg attacgcgcg atttccagca
    30781 ctgcatcggt aaaggcgtta caggctgctt tacacgctgc ctggctaccg gttaaaaatg
    30841 ctgccgagta gttggtttca gacggtggcg ggacataggt cgccagctgt acgtcggcag
    30901 acttcagggc tgcatcaatg ccataggtcg cttccagcgg cggtgccacc agatacgcca
    30961 tcggatcgcc aagcgtgatc ccggcggttg atgagagata agaaccggta cgcgaaacta
    31021 catgtgccag gaatgccgta tcttgcgcgt cattagccca ctggaaagct gcgccatttt
    31081 caatatgcgc aatcatcgca tccagacctg cacgcacttc cgccgggttt ggcccaccaa
    31141 gcataatcag cacttcaccg gcagtcggtg acgggccgtg tgcagcgccc gcatatagcg
    31201 agcggccata caccacttcc accatcgcct gcttggtcgc ttcatcagcg gcaatgtagg
    31261 tgacgtcatc ggagtcggca gaaatcagac cgaggctacg aatatgcggc ggcaatttca
    31321 gttcacgcgc aaaatcggcg ttaacggagg caatcacccg catagcggtg acagacggtc
    31381 gaatcaaatc taaagctggc atgatgcctc cttatcgggt catgttgatg ccggatgctt
    31441 tctgctccag catacgtttc gccaaattca caatgacggc tgcggcttca accggcggcg
    31501 tgccgccctg gtgaatgtta gagatacagg tgcgatcggc ctcaacggtg gtcgccatac
    31561 gcggcgagta aacggcgtag caggagaggc tttctgactg acccaacccc ggacgttcgc
    31621 ccaccagcag gatcactact ttcgcgccga ggatctcacc gatctgatct tcaatcttca
    31681 cgcgaccata acgaacaaag aatggtgtac cgactttcag cccggcctgt ttcaggcccg
    31741 ccatcagcgg tggcaggatc tcttcgtagt tcacggtgat cgcatcagtt gacaggccat
    31801 cagaaataac gacctgtacg tccggattgg caacacactg cgctttcagc gcttcaacag
    31861 cttctgcaca caggcggcgg cccatatccg ggcgagtcag gtagagattt ttgtcgctaa
    31921 tttcagagcg tacttccagc aagccctgcg ctttcaccca ctcttccggc acttctttca
    31981 gtacggtatc tttcgaacga gagtgatcgg ccaggaaacg cagcagcgcc tgcgtacgtg
    32041 gacgcggacc ggcacgaccg gtacaaacgc gggccacggt gctgcggcgc agttctgtta
    32101 atacgtctgc gcgatgcgga ttttcaacac caatccacgc ttttgcttca gcggaaccta
    32161 aatccagcgc acagctttcc gaggtcaccg gtgccgcaca gttggtggtg gcgcactttg
    32221 cttctgacgg cgcgggggcc gcttgtccca ttgacgccat cacgctgcgt acaatttctt
    32281 caatctgttt ttgatccatg atatgttatc tccgcgtcat cagaagaaca gtgacggatc
    32341 gcccgcccgt ttggtcaggc gaccgtttgc cataatgccc atgctttcca gccagcgttc
    32401 aaactccggt gacgggcgca ggttgagtaa ctgacgcaca gtggcggtat cgtggaatgc
    32461 ggtggtctgg tagttgagca tgatgtcatc acctagcggc atccccatga tgtagttgca
    32521 gcctgcggtg gcgagcagga tcatcaggtt ttcgttgagg ttctggtcag cgtcagcgtg
    32581 gttggtataa cagcagtcac agcccataga gatgccgctc agcttgccca taaagtgatc
    32641 ttctaagcca gcacggataa tctggcggtc gttgtagaga tactccggcc caataaagcc
    32701 gaccacggtg ttgacgataa acggatcgta atgacgcgcc agcccgtagt tacgtgcttc
    32761 catcgttacc tggtctgcgc cgaagttagc gccagcggat agcgcagagc cttgtccggt
    32821 ttcgaagtag aggcagtttt ccccggcgat acggttgaac tccgcgccca ctgcgcgcgc
    32881 ttcgtcgagc atcgccagct ccacgccaaa ctctttcagc cctttttcgc tgccacagat
    32941 actctggaaa atcagcccgc ccggtgcgcc acgacggatc gcttcgatct gggtggtgac
    33001 gtgcgccagt acacagccct gagttgggat gttgaatttg tcgatcacgc catagatggt
    33061 atccaacacg cggcttaagt tttccacgtc gtcagtcacc gggttaacgc cgatcaccgc
    33121 atcgcccacc ccgaaggaaa gcccttcgta gatttgcgcg gcgatacttt gcacgtcgtc
    33181 acgggtgtca tttggctgca aacgggcgct aaaggtgccc ggaataccga tggtggtatt
    33241 ggcctttttg attaccggca ttttcttcgc gccgtagatc aggtccgcgt tggagcaaat
    33301 cttcgctacc gccgcgacca cttccgaggt cagccctttg cgggtaaagg caatgtcgtc
    33361 cacgctggtt tcatcgctca gcacatactc acgcagttcg ctgatgctcc agtttttaat
    33421 ctggttgtag gccgtttcgt taacatcgtc ctgaatcagc cgcgtcacgc agtcatcttc
    33481 ataggcaatc accggattat tgcggatgtc cgctacggtc atttccgaca acacctgctt
    33541 tgccgccacg cgctcctgtg agcttgccgc tgcgacgccc gccagcacat cccccgaacg
    33601 cagttcgttg gctttagcca gcacctcttt tacatcctta aactgatata cattgccgaa
    33661 caatgtggtc tttagtttca taagtcgttc cctcaggaag gaaatgcgag tgatttcacc
    33721 gtcaccggca caaccgatcc gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg
    33781 cggacaatca cttcgtcaat gactgccaac gggagttgtt gtagctgtgg gcgcaacaac
    33841 atgcccagag ctttaccaaa gtcctgcccg gccaccacca gcaggggatg cggattcgga
    33901 aaacgcgcga cgaaatcgac caacgcgttg atgaccgtca gtaccgcggc gtaacgcaca
    33961 ggcagcgagg cgggaagcgc cagcacgtac gcgtcagttt tgggatcaag atccagctga
    34021 atcagcgcct gttgccaggc ccccaccaga tccgtttcat caatcgggat cgccaccggc
    34081 aaattgcgca gcggcagttg tacgccctca agccagattg tgctgccaga gagcgaaagg
    34141 gtatgcgcgc ccgcgccaat caccgtggcg cgcacggttt gcgccggaaa ctgtacgttc
    34201 atctcacgca ggcgcggatg gtcatgcagc gcagttgcca gcagcgggcc aatatcggca
    34261 aaacagaacg ggtcggcggg ctggtggcga taacattcgc ccacgccgcc agaaagcgta
    34321 atgatttcgg gcgtaacacc tgcgggcagc aaaccggttt gcatcaatgc ctgcgcgagc
    34381 ggtgagagcg ttccgtcaat cacttcgaca atcagttctg ccatccgccg ggtcacctgc
    34441 accagctgcg cgccggtcag cgaacgggcg tcggtgcctg caccgaagca ctcatccaca
    34501 atcatctgcc ccggtttatg agcgtaaacc acgcgcccgt ggctgtcggt ttccagcagg
    34561 cgaccaccga cgttgaggca ggcagtgccg ctgatttttc cggcatcgaa cagggcgtag
    34621 ttcgcggtgc caccgccgat gtcgatattc agtacccgac acagccgttg ttcagaaagg
    34681 gtttgtgccc cggctccgtg accggcgatc acggattcga ggtgcggccc ggcgctggca
    34741 acgacaaaat cgcccagcga ctgagagagc gccatcaccg ccgggcgagc attgcgggtt
    34801 ttcgcgcttt caccggtgat gatgatggca ccagaatcaa cgctttccgg ctcaataccc
    34861 gcagcatgat attgctcgag tattaaggtt ttcagttccg cttcttttaa accgccctgt
    34921 ttatcgacag gggtaaagaa caccggactt tgccagctaa tttcgcgttt aatgaattcg
    34981 tagcgcggca cctgcgacac cgccgcacgg ttaaccagct ccagccggga gaaaatcacc
    35041 tgggtggtgg tggtgccgat atcgataccg acgctcaata gctggcgagt gttcacgatt
    35101 gtgcctccgc ttcggtttta gtcgcggtcg cgtcttcttt tggcaccagc atcatcgcca
    35161 cgccaatcgc cgttacgccg ccgatcaact tgccgacaat catcgggaag atcatggcgt
    35221 tcatgttggc agcggcgaag cctaagtggt cgcccagggc gaaagcagcg gaaacggcga
    35281 aggcgcagtt gatgactttg ccgcgggtat ccatctgctt catcatgccg aacatcggga
    35341 tgttgttggc aagcgttgcc accatgccgg ctgccgcgat gttgttcata ttcagtactt
    35401 taccgacgct catcagcggt ttttcaaacc agcgagtcag cagcagcacc atcggatacg
    35461 cccctaacag aacgcaggag atagaaccga taacttcaat ggcgcgcatc acctcaccgg
    35521 gtttatcgcc aggggccata aagataggat ccagaccggg gatcagttcc cagccaagca
    35581 ggaatttcac taccgcagcg gcaagaccga gggtgatcaa tgcaacgagg aatttggcga
    35641 agatctggaa gccgttgatc attttttccg ggatgaattt cagccccagc gccaccagaa
    35701 tcgcaacaat gatcaccggg atcatgttca tcaggatcag ggcgaaagtg aattccaccg
    35761 gctggccgtt gatctgcaca ccggagtaca tagcaaccag accaccagcg atacaaccaa
    35821 tcggaatggt cacaatgccc gccagcacgc cgagcgccag ataacgacgg tcagaaggtt
    35881 cgataatgcc gagcgccacc ggaatggaaa acacaatcgt tggccccatc atcgacccga
    35941 gaattaaccc agagtatagc cacgcggcta cgtcgccgcc cgccagctct ttggcgagga
    36001 agaagccgcc catatcgcac gccagcagtg ttccggcgaa catcgatggg ttagcgccga
    36061 gcatttcgta aaccggaata attaccggcc cgagaacgtg agccagtacc ggtgccagcg
    36121 cggtcatacc gaccatcgcc aggcccagtg cgcccattgc cataaagcct tcttcgaact
    36181 gaccgcctga tccttcgata cttttaccga acttaccgag gaaacgagca gaaccgccga
    36241 actgcgacag gatcctgtct acggcagcta tcagcataaa gaacatcatg atgtacatga
    36301 tgatttcgtt aattcccatc gcctttactc cctgttagtt gttatttatt ggcggatgcg
    36361 gcgtaaacgc cttatccgcc ctacatgtgc aatcccgtag gctggataag acgcggcaag
    36421 cgtcgcatcc ggcaattgca ccgcgccact ggcggatgcg gcgtgaacgc cttatccgcc
    36481 ctacatgtgc aatcccgtag gctggataag atgcggcaag cgtcgcatcc ggcatttatt
    36541 gcgccgctgc gtacaggccg acaatctgct ccaggctggc ggtacgcggg ttactgcgca
    36601 gacaaatatc ttccagcgcg gcctgcgccc atgcgccgta atgcgcagat gtcgcaccaa
    36661 catcgcccag tcgtttacca atcccaactt ccgcaatcag ctcacttacc gcgttaatag
    36721 cgtcacgatc gtcggatttt ttagttcgca gtgcccgacc aatctgacta aagcgttcac
    36781 gacaaaccat ccggttaaat tccatcaccg ttggcagcaa catggcgttc gcgagaccgt
    36841 gcggaatatg cagcgccgcg cccggctgat gcgccatcgc gtggcacaac ccaagacccg
    36901 cactggaaaa cgccattccc gccatacatg aagccagcaa catgctctcg cgcgcggcaa
    36961 ggtcgtgacc gtagcccacc gctttcggca gcgatttgcc aatcatcgca atggcaccaa
    37021 tcgccagact gtcggtaaac ggtgtagcgt tcagggcgct gtatgcttca atggcatggg
    37081 ttaacgcatc aatgccggtc atcgccgtga catgcgacgg cacaccttcg gtcaatgcgg
    37141 cgtcgaggat cgccacatcc ggcatcagcg aggcatgggc taacacctgc ttgcgcccgc
    37201 tcaccgcgtc gataatcacc gttacattgg tggtttcaga gccggttccg gcggtagttg
    37261 gaatggcaat cagcggcaag cgcggttgca gaacgctggt ttctgacatc tctgccagcg
    37321 tgctatccgg gttcgtcacc agcaacgtca cggctttcgc cgcatccagc accgagccgc
    37381 cgccaaacgc gatcacccca tcacagcctg actcacgcaa ctgcgccacg gctgcacaca
    37441 cgtcggtaat gcacggttcg cccaccggac atggccagag cgtcatggcg atacctttaa
    37501 cggtcaggct acgcgtcagc ccggcggtca tccctgcctg atgcaaaaag ctgtctgcca
    37561 tcacgaacag atgtttcagc ccacgcgttt gcgcttgctg tccgcaactg ctcaccgagc
    37621 ccggaccgca aagcgtcacc ggtggaacgc taaatgtttt tacccgttgc agattcaggg
    37681 tatcgaacgc ctgaaagagc gcggtctgca attcattttg catatagccc ctccgctttc
    37741 tctcgcccac tactggcgat cgccagcggg gtcataaaca aactgtgctg cggtaaatgc
    37801 acctgtaacg ccgggaattg tttgcgaaac agttccgcca cgcccggttg catacaagag
    37861 ccgcccgcca gccataaatc agtaatgccc tgcccttcaa tatgccgggc gacaatgtcc
    37921 gccatttttt cgtacaccgg tttcaccgca ggccaaatct cttcaccgtg accgcgcttg
    37981 tactgctccg cctcttccag cgaaatacgg cgatttccgg cgagagtcag agagatgtga
    38041 tgtccgccgg tggcttcatc cgccgagtac gtcaccttgc cctttttcac gatggcaatg
    38101 ccggtagtgc cgccgccgat atccactaca ccggcgttgt ccagttgcag cagatccgca
    38161 actgcggttg gctcatccag cacatggctc acttccagtc cggcagattc cagcacgttg
    38221 atggaaatac gcgggtccgt acctggagga aatgaagtcg ccgcatggct aaaacgacga
    38281 ccgaattgct gctcgagcgt gtcgagatgg cgacgaacaa tggtgacagc gccgaagaaa
    38341 tcccagacga tgccgtcgcg aaccacatcg gcccagtcga ggcataccgc cactggctga
    38401 ccgtcgcggt cgacaaccat cgacaccaca tcgcaggtgc ccagatccac ccccagccac
    38461 agcggagatt ccgtcgcggc gggcgtctgg ttacacagcg ttgccgccgt ttgcagacgt
    38521 ggggtgagcc attgttcgtc gtgcgccatc tgttactcct taaacaatgc gaaacgcatc
    38581 gactaataca cagcgacgca gacggacaaa cgtacgcgcg ctggttaccc cttcaccggt
    38641 tggcgtggtg atggtcatgg tggtccagcc ttccccgccc agcccc
//
LOCUS       DBBGLQ010000041        38461 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.041, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000041 DBBGLQ010000000
VERSION     DBBGLQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.041"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(136..327)
                     /gene="yfgG"
                     /locus_tag="ACIYSB_002743"
     CDS             complement(136..327)
                     /gene="yfgG"
                     /locus_tag="ACIYSB_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGC5429764.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            345..497
                     /locus_tag="ACIYSB_002744"
     CDS             345..497
                     /locus_tag="ACIYSB_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429765.1"
                     /translation="MFHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            679..2922
                     /gene="pdeF"
                     /locus_tag="ACIYSB_002745"
     CDS             679..2922
                     /gene="pdeF"
                     /locus_tag="ACIYSB_002745"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGC5429766.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMLVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2961..4502)
                     /gene="ppx"
                     /locus_tag="ACIYSB_002746"
     CDS             complement(2961..4502)
                     /gene="ppx"
                     /locus_tag="ACIYSB_002746"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGC5429767.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4507..6573)
                     /gene="ppk1"
                     /locus_tag="ACIYSB_002747"
     CDS             complement(4507..6573)
                     /gene="ppk1"
                     /locus_tag="ACIYSB_002747"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGC5429768.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6744..7382)
                     /gene="purN"
                     /locus_tag="ACIYSB_002748"
     CDS             complement(6744..7382)
                     /gene="purN"
                     /locus_tag="ACIYSB_002748"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGC5429769.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDMVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7382..8419)
                     /gene="purM"
                     /locus_tag="ACIYSB_002749"
     CDS             complement(7382..8419)
                     /gene="purM"
                     /locus_tag="ACIYSB_002749"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGC5429770.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8744..9370
                     /gene="upp"
                     /locus_tag="ACIYSB_002750"
     CDS             8744..9370
                     /gene="upp"
                     /locus_tag="ACIYSB_002750"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGC5429771.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9456..10745
                     /gene="uraA"
                     /locus_tag="ACIYSB_002751"
     CDS             9456..10745
                     /gene="uraA"
                     /locus_tag="ACIYSB_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGC5429772.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10840..11541
                     /gene="hda"
                     /locus_tag="ACIYSB_002752"
     CDS             10840..11541
                     /gene="hda"
                     /locus_tag="ACIYSB_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGC5429773.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11679..12038)
                     /gene="arsC"
                     /locus_tag="ACIYSB_002753"
     CDS             complement(11679..12038)
                     /gene="arsC"
                     /locus_tag="ACIYSB_002753"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGC5429774.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12059..13522)
                     /gene="bepA"
                     /locus_tag="ACIYSB_002754"
     CDS             complement(12059..13522)
                     /gene="bepA"
                     /locus_tag="ACIYSB_002754"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGC5429775.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13735..14796
                     /gene="yfgO"
                     /locus_tag="ACIYSB_002755"
     CDS             13735..14796
                     /gene="yfgO"
                     /locus_tag="ACIYSB_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGC5429776.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14834..15683)
                     /gene="focB"
                     /locus_tag="ACIYSB_002756"
                     /pseudo
     CDS             complement(14834..15683)
                     /gene="focB"
                     /locus_tag="ACIYSB_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(15705..17717)
                     /gene="hyfR"
                     /locus_tag="ACIYSB_002757"
     CDS             complement(15705..17717)
                     /gene="hyfR"
                     /locus_tag="ACIYSB_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGC5429777.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLTRIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIECLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17747..18160)
                     /gene="hyfJ"
                     /locus_tag="ACIYSB_002758"
     CDS             complement(17747..18160)
                     /gene="hyfJ"
                     /locus_tag="ACIYSB_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGC5429778.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(18153..18910)
                     /gene="hyfI"
                     /locus_tag="ACIYSB_002759"
                     /pseudo
     CDS             complement(18153..18910)
                     /gene="hyfI"
                     /locus_tag="ACIYSB_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18907..19446)
                     /gene="hyfH"
                     /locus_tag="ACIYSB_002760"
     CDS             complement(18907..19446)
                     /gene="hyfH"
                     /locus_tag="ACIYSB_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGC5429779.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(19456..21123)
                     /gene="hyfG"
                     /locus_tag="ACIYSB_002761"
     CDS             complement(19456..21123)
                     /gene="hyfG"
                     /locus_tag="ACIYSB_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGC5429780.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21113..22693)
                     /gene="hyfF"
                     /locus_tag="ACIYSB_002762"
     CDS             complement(21113..22693)
                     /gene="hyfF"
                     /locus_tag="ACIYSB_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGC5429781.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRRLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22698..23348)
                     /gene="hyfE"
                     /locus_tag="ACIYSB_002763"
     CDS             complement(22698..23348)
                     /gene="hyfE"
                     /locus_tag="ACIYSB_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGC5429782.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNDLTALKG"
     gene            complement(23360..24799)
                     /gene="hyfD"
                     /locus_tag="ACIYSB_002764"
     CDS             complement(23360..24799)
                     /gene="hyfD"
                     /locus_tag="ACIYSB_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HGC5429783.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVVALAITGVPPFNGF
                     FSKFPLFVAGFALSVEYWILLPALILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24816..25763)
                     /gene="hyfC"
                     /locus_tag="ACIYSB_002765"
     CDS             complement(24816..25763)
                     /gene="hyfC"
                     /locus_tag="ACIYSB_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGC5429784.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRE
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25774..27791)
                     /gene="hyfB"
                     /locus_tag="ACIYSB_002766"
                     /pseudo
     CDS             complement(25774..27791)
                     /gene="hyfB"
                     /locus_tag="ACIYSB_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27791..28408)
                     /gene="hyfA"
                     /locus_tag="ACIYSB_002767"
     CDS             complement(27791..28408)
                     /gene="hyfA"
                     /locus_tag="ACIYSB_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGC5429785.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKNGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28661..29131)
                     /gene="bcp"
                     /locus_tag="ACIYSB_002768"
     CDS             complement(28661..29131)
                     /gene="bcp"
                     /locus_tag="ACIYSB_002768"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGC5429786.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29131..29703)
                     /gene="gcvR"
                     /locus_tag="ACIYSB_002769"
     CDS             complement(29131..29703)
                     /gene="gcvR"
                     /locus_tag="ACIYSB_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGC5429787.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29849..30727
                     /gene="dapA"
                     /locus_tag="ACIYSB_002770"
     CDS             29849..30727
                     /gene="dapA"
                     /locus_tag="ACIYSB_002770"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGC5429788.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30744..31778
                     /gene="bamC"
                     /locus_tag="ACIYSB_002771"
     CDS             30744..31778
                     /gene="bamC"
                     /locus_tag="ACIYSB_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGC5429789.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31991..32704
                     /gene="purC"
                     /locus_tag="ACIYSB_002772"
     CDS             31991..32704
                     /gene="purC"
                     /locus_tag="ACIYSB_002772"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGC5429790.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32872..33735
                     /gene="ypfJ"
                     /locus_tag="ACIYSB_002773"
     CDS             32872..33735
                     /gene="ypfJ"
                     /locus_tag="ACIYSB_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGC5429791.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33750..35765
                     /gene="tmcA"
                     /locus_tag="ACIYSB_002774"
     CDS             33750..35765
                     /gene="tmcA"
                     /locus_tag="ACIYSB_002774"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGC5429792.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFEHTLKLRDALP
                     GDWLWISPRPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNEAILWRQNQPFSL
                     AHFTPRTDWYPATGAPQPEQQQLLKQLMTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     RAIVTAPAKASTDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWQSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGNNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQLFH"
     gene            35839..36537
                     /gene="ypfH"
                     /locus_tag="ACIYSB_002775"
     CDS             35839..36537
                     /gene="ypfH"
                     /locus_tag="ACIYSB_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGC5429793.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGNWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36647..36847)
                     /gene="ypfN"
                     /locus_tag="ACIYSB_002776"
     CDS             complement(36647..36847)
                     /gene="ypfN"
                     /locus_tag="ACIYSB_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGC5429794.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36875..38002)
                     /gene="dapE"
                     /locus_tag="ACIYSB_002777"
     CDS             complement(36875..38002)
                     /gene="dapE"
                     /locus_tag="ACIYSB_002777"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGC5429795.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38006..38362)
                     /gene="yffB"
                     /locus_tag="ACIYSB_002778"
     CDS             complement(38006..38362)
                     /gene="yffB"
                     /locus_tag="ACIYSB_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGC5429796.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLND
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCVPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9404 a         9900 c        10106 g         9051 t
ORIGIN      
        1 acaggataaa catcagtcag gataaagacg taaaatttaa gatgaaagaa tatctgccgc
       61 tttgataatc ccaaacagca aaagcatccg acagcgaagt gccgttatgt tctgtgaaag
      121 tggagaaggt aaccgctaac gttgtaccgg tgattcgacg gagagtgtgg attgctgagc
      181 ttcttttttg ctctgatggt gctgccaggc accgacggac gagtagataa aacggccaaa
      241 gaagaagata aagctgatga gcagtacgat acgggtcatg cgactgttaa atcggtgtcg
      301 ttttcgcata ctggttgcct gactcacaaa aggttccttg aagtatgttc cacgctgtgg
      361 acggtactta cattaaggca caacaggaca aaatggtcaa ttcttcgtta tgtaaaaaag
      421 cgtcagtgga tacatatttt aatgttatgg aagttaattt aattatttac aatgatgatg
      481 taataatgat gaattaacat gataaacgta ataattcatt aatcagggtt attttgtttg
      541 atatatatca attggtactt tcatatgaca cttagaatca atgctctcta tagtgatgaa
      601 taatcatcat tcgaagtcag gtgggatgcc tgtctgaata cacctccttc aggatgtggg
      661 ggattctgct gagcatctat gaaactgaat gcaacttata taaaaatacg tgataaatgg
      721 tgggggcttc cgctgttcct gccttcttta atcttgccca ttttcgccca cattaatact
      781 ttcgcgcata tttcttccgg tgaggttttt ctcttttatc tgcctctggc actgatgatc
      841 agcatgatga tgtttttcag ctgggcggca ttgccaggga tcgccttagg gatttttgtc
      901 cgcaaatatg cagagctggg tttttacgaa acgctatcat taacggctaa ttttattatc
      961 attatcattc tctgttgggg cggttacagg gtctttactc cccggcgtaa caacgtttca
     1021 catggtgata cccgtttaat ttcccagcgt atattctggc agattgtgtt tcctgcaacg
     1081 ctgtttctga tacttttcca gtttgctgca tttgtaggat tactggcgag cagagaaaat
     1141 ctggtcggtg tcatgccttt taacctcggg actttaatca attatcaggc cttactggtg
     1201 ggtaatctga tcggtgtccc gctgtgctac ttcatcattc gggtagtgcg aaatccattt
     1261 tatttacgta gctattattc gcaattaaaa cagcaggttg atgccaaagt caccaaaaaa
     1321 gagttcgcgc tctggctact ggcattaggt gctttattgt tgctgttatg catgccgtta
     1381 aatgaaaaaa gcacaatttt tagcaccaat tataccttgt cattattgct gcccctgatg
     1441 atgtggggag cgatgcgcta tggttataag ctgatttcgc tgctctgggc ggtcgtgttg
     1501 atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta taccacgcag
     1561 ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta tatggctgta
     1621 ctggcaaccc gtcagagagc ggtagtcaga cgcattcagc ggcttgcgta tgtggacccg
     1681 gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc cccctggtct
     1741 gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaagaacta tggcatcatg
     1801 ctgcggattc aatacaagca aaaactttct cactggctgt cacccttgct ggaaccgggt
     1861 gaagatgttt atcagctttc gggtaacgat ctcgcgctgc gactgaatac agaatcgcac
     1921 caggagcgca ttaccgcact ggatagccat ctcaagcaat ttcgtttctt ttgggatggc
     1981 atgccgatgc aaccgcagat tggcgtcagt tactgctatg tgcgctcgcc agtgaatcat
     2041 atctacctgc tgctgggaga gctaaatacg gtcgccgaac tttccatcgt gaccaacgcc
     2101 ccggaaaata tgcagcgtcg cggggcaatg tatttgcaac gcgaattgaa agataaagtc
     2161 gcgatgatga atcgactaca gcaggcgctg gaacacaacc attttttcct gatggcccag
     2221 ccgattaccg gtatgcgtgg tgatgtttac catgaaattc ttctgcgcat gaaaggtgag
     2281 aatgatgaac tgatcagccc cgatagcttc ttgccggtcg cgcacgaatt tggtttatcg
     2341 tcgagtatcg acatgtgggt cattgagcat acgctgcaat ttatggctga aaacagagcg
     2401 aagatgcccg ctcaccgttt tgctattaat ctgtctccaa cctcggtatg tcaggctcgt
     2461 tttcctgttg aagtcagtca gttgctggct aaatatcaga ttgaagcgtg gcaacttatt
     2521 tttgaagtca ccgaaagtaa tgctctgacc aatgttaagc aggcgcaaat caccttgcag
     2581 catcttcagg aattaggctg ccagattgcg attgatgatt tcggcaccgg ctacgccagc
     2641 tatgcgcggc ttaaaaatgt gaatgccgat ctgcttaaaa ttgacggcag ttttatccgc
     2701 aatattgtgt caaatagtct ggattatcag atagtggcat cgatttgcca cctggcgcga
     2761 atgaagaaaa tgctggtagt ggcagagtac gttgaaaacg aagagatccg cgaggcggtg
     2821 ctctctttgg ggatcgatta tatgcagggt tatcttattg gtaagccgca gccgttaatt
     2881 gatacgctga atgaaatcga acccattcgc gaaagtgcct gaataatgcg ggccgacatt
     2941 tctcgtcggc ccgcaaagta ttaagcggcg atttctggtg tactttcttc ttcaattttc
     3001 aaccgccagc cagccacgcc ttcccagtat tcttgctcct tttccagatc aagcagtacc
     3061 agcgcattct gactaaacca gtcatgcggg aaacgcagtg tccagtgact gtcatcagta
     3121 atcagcgtca atgttggcgg tgtggtggtt gcctgacgtt gattgttgag taatacgcca
     3181 aggcgcaata gctgtatcag tggcaggaac tgtttcttct taaacaaggt aaagcgcggc
     3241 agatcgtcga gcttaatcgc tttacggtga tagcgcacca gtgtcgccat catcagctgc
     3301 tgttcctgat taaaacccgg caagtcactg ttttgcagaa tataagcgga gtggcgatgc
     3361 aaaccgctgt ggttgatatt caacccgacc tcatgcagca tggcggccca tcgcagtagc
     3421 gcctccagtt gcggatgcgc cagcttcggt tgctgttccc gccactgttc gtacatttgc
     3481 atagtggtat ccagcacccg tcgggcctgt tcgctgtcga tgtgatactg gttggcgagg
     3541 ctgctggcgg tgcgactacg cacatcctga tgacggaaac gtccttccat ttcatacagt
     3601 acgccttcgc gtaacgcccc gtcagaaagg cgcagttcac ggatggctaa agcatcaaac
     3661 acaccgcata aaatcgccag tcccggaacg aagactgttt tccgctcttc ggaaagaccc
     3721 ggtaaactca gcgatgcgaa attacggtga cgtaaaactt cttttaccag tttttccaga
     3781 cgttccgggg taattatccc gtctttctcg cccatttcca ttaacacttc atgggcggct
     3841 tttatggtac cggaagcgcc cattgcaacg ttccagccct gaatacggaa ttgccaggtt
     3901 aaagtttcca gtttttgtgc tgccgccatg cgagcgcgct gaaaattctc tttattgatg
     3961 accccgccag gaaaataaag ctgggcaaag ctgacacaac ccatccggcg gctttcaacg
     4021 agaataggtt cgaaattttc accaatcacc agttccgtag atccgccgcc aatatcaata
     4081 accagtttgc gacctttttc cggttgggta tgttccacgc ccataaaaat cagacgggct
     4141 tcttcattac cggaaataat ttcaatcggg tagggaatga ccttttccgc gcgtttcaga
     4201 aagtcagtgg cgttcagcgc ctgacgcagc gtatgggtac caactataca gacgctggca
     4261 ggagaaaacc cttgtagccg ttcggcaaac agcgacagac agtttaaacc gcgcgtcatt
     4321 gcctcttcac tcaacatatt atctggcccc aggccgtccg ccagatgcac ccgctgtttc
     4381 aggcggccaa taatctgcat ggcaccatct accacacggg ctatgaccat gtgaaaactg
     4441 tttgaaccaa gatcgaccgc agcaaactcc tgcggacgag gggatttatc gtgtattggc
     4501 atagggttat tcaggttgtt cgagtgattt gatgtagtca taaatcgcca actgcgcccg
     4561 tactttgcgg cgattgccgc ggggaacgta gcgattactg agttctttat cgatataacg
     4621 tgctttgacc gtatcgctga acaatatgtc gatgatgtcc agtacccgct gcttcaggcg
     4681 cggatcgagc agcggcgtcg ccacttcaat acgataatca atattgcgcg tcatccagtc
     4741 ggcggaagaa aggtagacct ttttatcgcc gccattttca aaaatataaa cccggtcatg
     4801 ttcaaggtaa cggtcaacaa tactgatggc acgaatgttg tcgctaatgc cttccagatt
     4861 ggggatcagc gaacacattc cgcgaaccag cagattaacc ggtacgccgg agctggaggc
     4921 cgcatacaga cgatcaacca ggcctttatc gacaaggtta tttagcttca gggtgatacc
     4981 actgggcagc ccttgctgcg cgttggcgat ctcgcggtcc accatttcat acaacaggcg
     5041 gcgggagttt tgcggcgaca ccattaaata atcaaatgtc accggacggt atgggttttc
     5101 aataaagtta aatacccgcc gtacttcgtt ggtgatgcgc gcatcggcgg tcagcaacga
     5161 atagtcagta taaagacgcg cggttttttc gttaaagttc ccggtcccga tgtgtgcata
     5221 acgcaccact tcaccgtttt ctttacgtga aatcaggaac agtttggcgt gaattttcag
     5281 ccccggcgca gagaagataa cgtgcacgcc tgcttcggtc aggcgcttcg cccagtgaat
     5341 gttggcttct tcgtcaaaac gtgcctgtag ctcaaccacc acggtcactt tcttgccgtt
     5401 gtgcgcagcg tggatcatcg agtcgatgat acgtgaatct tttgccacac ggtaaatgtt
     5461 gattttaatc gccagcacgc tcggatcgaa cgaggcctga cgcagcagtt ccagcacatg
     5521 ctcaaaggtg tgataaggat aatagagcaa cacatcgcgt tcgcgaatgg catcaaaacc
     5581 attgcggaac tgggctttat caaaccaaat gtggcgtaaa cgcggcagtg gtttgttcac
     5641 cagattggct ttgccgacat tggggaaatt aataaagtct ttaaaattat gataacgacc
     5701 gccggggacg atggagtcgt agcgggaaat agtcagtttt tcgcgtaaca cttcaaccag
     5761 cgcattgggc atatcgcgct gataaacaaa acgcaccggc tcagcagtta aacgctgctt
     5821 gagactggaa gacatcaact ccatcaggct ggcttccatc tcatgcacta aatcgtattc
     5881 ggcatcgcgg gtcatcttca ttgaataggc attcagcgcg tcataatcaa agaagccttt
     5941 gaaaatatca tcaaggcagt aacgcagaat gttatccaga agaatcatcg gcttgcgtcg
     6001 acgcggcgct tctggcggta aattcacaaa gcgcggcact ttatctgatg ggatctccag
     6061 cagcgcgtaa cggatggtat cgccacggat aatttccacc gccaaatagg tgtaatcatc
     6121 tttcaggaac tgcactaagt cagtgtcagg attgattaaa atcggcgtaa tgtgctgacg
     6181 cagatactgc ttaaaataat gacgcagcca gttttgttga ttgacggaga gctggcgttc
     6241 attaatcagg aagatctggt tgcgcgccat ctccagcaat agctcgttgt agaggccgtc
     6301 gaattcctga tcggctttca gcacccggga ctgaattttg cccagtaaat ggcgggaatg
     6361 agagttggag ccttgttctt cgctaataat gatgcgtcgc ttcagttcag cgaagcggac
     6421 tttatagaac tcatcaaggt tattggaata gatccccagg aaacgcatcc tttcaatcag
     6481 cgggttagat ttgtccgccg cttcctgaag cacgcgttca ttgaacgata accaactgag
     6541 ctctttttcg atgtatagct tttcctgacc cattaccact tttactccgt tttattcacg
     6601 ggacactgcc tggatattat ggcgagcaat attggcagat gtccaactat gccagaaaag
     6661 ggggaaaaaa actgctggag cttgcgattt acaggtgaat tgaaaaacgc cagcggcaga
     6721 gctggcgctt taactacggg gcattactcg tcggcagcgt agccctgcgg cggcagacgt
     6781 tgaccatcca gccacgcggc gttttcgtgc attttcagac gaccatcggc aaaccagcta
     6841 atcaccagtg gataaatagc gtgttcctgg gtttgcacgc gggcggtgat gtcatcttcc
     6901 gtatcaccag caaataccgg gactttcgcc tgtaaaataa ccgggccacc gtccagttca
     6961 tcggtgacga aatgcaccga tgtaccgtgc tcttcatcac cattttccag cgcctgacga
     7021 tgggtgtgta atccgggata tttcggcagc agagaagggt gaatgttcag caaacgcccg
     7081 gcatagtggg agacaaacgc cgggctgaga atgcgcataa aaccagccag cacgaccata
     7141 tcgggtgcgt acatgtcgat ttcatgaatc aactcccggt cataggcttc acgactgtca
     7201 aacgcgctgg cgatgagcgt atgcgttgca atacccgcct ggcgggcgcg ttcaaggccg
     7261 aacgcgtcgg ccttattgct gaaaactgcc cgtacggtgc ctttaatttt gttagtttta
     7321 caggcgtcaa taattgcctg taaattactt ccgttgccgg aaataagcac cacaatattc
     7381 attattcgat aaccacgcgt tgttcggaat cagaggcttt gatgataccg attttccacg
     7441 cgttttcacc gttggcgttt agcagggcga gggctttgtc cacttccgga gcaggcaggg
     7501 cgataatcat cccgacgccg cagttgaagg tgcgatacat ttcatggcgc tcaacgttac
     7561 ctgccgtttg cagccagttg aacacttccg gccactgcca ggaagattca tcaatcactg
     7621 cctgagtatt atctggcaat acgcgtggaa tgttttccca gaagccgccg ccggtcaggt
     7681 gcgcaatggc atgcacatcg accttttcaa tcaactccag cactgacttc acgtaaatgc
     7741 gggtcggtgc cagcagatga tcggctaatg gcttaccatc aagttcggtg gtttgcggat
     7801 cacaaccgct gacttcaaga attttgcgca ccagcgaata gccgttcgag tgcggaccgc
     7861 tggaaccgag tgcaatcagc acatcgccgt cgctgacttt agagccgtcg atgatttctg
     7921 atttttctac cacgccaacg cagaaacccg cgacatcgta atcctcaccg tgatacatcc
     7981 ccggcatttc tgccgtttcg ccacccacca gtgaacagcc cgattgcaga catccttccg
     8041 cgataccgct gatcaccgct gaagcggtat caacatccag ttttccggtt gcgtaatagt
     8101 cgaggaaaaa cagcggctct gcaccttgca ccaccaggtc attaacgcac atggcgacca
     8161 gatcaatacc aatggtgtcg tgacgtttta agtccattgc cagacgcagc ttggtaccta
     8221 cgccgtcagt gccggaaacc agcacgggtt cacgatattt ttgcggcaat gcacacagcg
     8281 caccgaagcc gcccagaccg cccatcactt ccggacgacg cgttttcttc actacgcctt
     8341 tgattcttcc aaccagagca ttacccgcgt caatatcaac accggcatct ttgtagctaa
     8401 gagaggtttt atcggtcact gcttgggtcc ccacgcgtta cttgcggtag aaaaataaaa
     8461 ttcggcgcaa ttctaacagg gaaagcaaac gtttgcgaga ctgctttaca caaccttttt
     8521 gcacgtcttt tccccaggcg cgcggcgaaa gaagacttgt gccagggcaa aggttagttt
     8581 tcggatggaa taatcttctt tcataaccat ttgaatataa aataacttta tctcaaaccg
     8641 ttatcatttt gactaaagtc aacgaaaata atattgccgc cttgaagaaa ggaggtataa
     8701 tccgtcgatt ttttttgtgg ctgcccctca aaggagaaag agtatgaaga tcgtggaagt
     8761 caaacaccca ctcgtcaaac acaagctggg actgatgcgt gagcaagata tcagcaccaa
     8821 gcgctttcgc gaactcgctt ccgaagtggg tagcctgctg acttacgaag cgaccgccga
     8881 cctcgaaacg gaaaaagtaa ctatcgaagg ctggaacggc ccggtagaaa tcgaccagat
     8941 caaaggtaag aaaattaccg ttgtgccaat tctgcgtgcg ggtcttggta tgatggacgg
     9001 tgtgctggaa aacgttccga gcgcgcgcat cagcgttgtc ggtatgtacc gtaatgaaga
     9061 aacgctggag ccggtaccgt acttccagaa actggtttct aacatcgatg agcgtatggc
     9121 gctgatcgtt gacccaatgc tggcaaccgg tggttccgtt atcgcgacca tcgacctgct
     9181 gaaaaaagcg ggctgcagca gcatcaaagt tctggtgctg gtagctgcgc cagaaggtat
     9241 cgctgcgctg gaaaaagcgc acccggacgt cgaactgtat accgcatcga ttgaccaggg
     9301 actgaacgag cacggataca ttattccggg cctcggcgat gccggtgaca aaatctttgg
     9361 tacgaaataa agaataaaaa taattaaagc cgactttaag agtcggcttt tttttgagta
     9421 aagcgcctat aacacataat acagaggata atactatgac gcgtcgtgct atcggggtga
     9481 gtgaaagacc gccactttta cagacaatcc cgcttagttt gcaacatttg ttcgccatgt
     9541 ttggtgcaac cgtcctggtg cccgtcttat ttcatattaa cccggcgact gtactgttat
     9601 ttaacggtat tggaacgctg ctgtatctct tcatctgtaa agggaaaatt ccggcttatc
     9661 ttggttccag ctttgccttt atttcaccgg tattgttact gttgccgttg gggtatgaag
     9721 tcgcgctggg cggctttatt atgtgcggcg tgctgttctg cctggtttct tttatcgtga
     9781 agaaagcggg gaccggctgg ctggacgtgc tgtttccacc tgcggcaatg ggcgcaatcg
     9841 ttgccgtcat cggtctggag ctggcgggcg tagctgccgg tatggcgggt ttactcccgg
     9901 ctgaagggca aacgccagac tccaaaacca tcatcatctc tattaccacc ctggcggtca
     9961 cggttttagg ttccgtgctg tttcgtggtt tcctggcaat tatcccgatt ttaattggcg
    10021 tgctggtggg gtacgcgctc tctttcgcaa tgggaattgt cgataccacg ccgattatta
    10081 atgctcactg gtttgcgctg ccaaccctct atacgccgcg cttcgagtgg tttgccattc
    10141 tgactattct gccagcggca ttagtggtta ttgccgaaca cgtagggcac ctggtagtaa
    10201 cggctaatat cgtcaaaaaa gatctgctgc gcgatccagg tctgcaccgt tcgatgtttg
    10261 ctaacggcct gtcgaccgtg atctccggct tctttggctc tacgccaaat accacttacg
    10321 gagaaaacat tggcgtgatg gcgatcaccc gtgtttacag tacttgggtt atcggcgggg
    10381 cggcgatttt cgctatcctg ctttcctgcg tcggtaaact ggctgccgct atccagatga
    10441 tcccattgcc ggtgatgggc ggcgtttcgc tgctgcttta tggtgtcatc ggtgcttccg
    10501 gtattcgtgt tttgatcgaa tcgaaagtgg attacaacaa agcacagaac ctgatcctga
    10561 cttccgtgat tttgatcatc ggcgtcagtg gcgcgaaggt aaacatcggc gcggcggagt
    10621 taaaaggtat ggcgctggcg accatcgtcg gtatcggctt aagtttgatc ttcaaactga
    10681 ttagtgtgct gcgccctgaa gaagtggtgc tggacgcaga agacgcggat ataacagaca
    10741 aataatgtac cgaccgggca gtgttgctgc ccggttcaaa catcatggga ttctgtggta
    10801 aacttctcgc gattttgtga aatcctggtt gaggtatctc tgaacacacc ggcacagctc
    10861 tctttgccac tttatcttcc tgacgacgaa acctttgcaa gtttctggcc gggggataac
    10921 tcctctttac tggccgcgct gcaaaacgtg ctgcgtcagg aacatagcgg ttacatctat
    10981 ctctgggcac gcgaaggcgc ggggcgcagc catctgctgc acgcggcttg cgcggaattg
    11041 tcgcagcgtg gcgatgcggt gggctatgtc ccgctggata aacgcacctg gtttgttccg
    11101 gaagtgctcg acggtatgga gcatttgtcg ctggtctgta tcgacaacat tgagtgtatt
    11161 gcaggcgatg agttgtggga gatggcgatt ttcgatctct acaatcgaat tctggaatcg
    11221 ggcaaaacac ggttgttgat caccggcgat cgtccaccgc ggcagttgaa tctgggatta
    11281 ccggatctcg cgtcgcgact cgactggggg cagatctaca aattgcagcc actttctgat
    11341 gaagataagt tgcaggcgct acagttacgc gcgcgtttgc gtggttttga actgccggaa
    11401 gatgtggggc gtttcttgct gaagcggctc gacagagaaa tgcgcacgct atttatgacg
    11461 ttggatcagt tggatcgtgc gtcgattacc gcgcaacgta agctgaccat tccgtttgtg
    11521 aaagaaattc tgaagttgta gataaggtgt ttattgtcgg atgcggcgcg aacgccttat
    11581 ccgaactacg cgaagttaat ccatagtatt cgtaggccgg ataagacgtg gcaagcgtcg
    11641 catccggcaa tgtgcttcaa ctagcgatgg agcacatctt aacccacgat ttccagtacc
    11701 tgctccggtg gacgaccaat ccgcgctttg ccattcgcga ccacaatcgg gcgctccatc
    11761 agcttcggat tatccaccat tgcctgaatc agcgcctctt cgctaagcga actgtcagcc
    11821 aggttcagtt ctttataaag atcctctttc tggcgcatca attcgcgggc gctattcatc
    11881 cccagcattt tcagcaaatc gcgcagcgtt gccgcatcgg cgggtgtctc aaggtagagc
    11941 accacttctg gctccacgcc gttttctttc agcaggttca gcgtttcccg gctctttgaa
    12001 cagcgtgggt tatggtagat ttttacctgt ttggtcattg ttcttccttt aatgcgaatt
    12061 acatcttggt ataaggctta aagcgttcct gcagctggcg caactggtcg atgcgcgcat
    12121 cgtaacgcgc ttgttgcagg ctgcctaatt tcacctgcga actggcgcta ctcaacaagg
    12181 aaatggcctg atcgagtcgt ccggcgagcg cataaccttc tgctcgcgca gccagctcct
    12241 gatcgcggtt atttagcgcg gcttccgcct gtgccagcaa atcccagccg ttgctgtcat
    12301 ctttattatt aaaggtgtag cgattgagaa tattggccgc ttcttgtggt tgaccgcctt
    12361 gcagataagc gttcgccagg ttgagctgca acaccggatt ggtgcgcaaa tcacgggcat
    12421 ttttcaggcg attgattgcc tcattggctt tgttttgccc aagatcgata tcggtagcca
    12481 gatcgagata ccatgcgttg ccaggttctg ccgccagtaa cggttgcagc gttttacgcg
    12541 cctcgtcgta tttattggct tccatcgcct gtaaagcacg accatattgc gccgctcgtt
    12601 gctgacgaac gtttcctttc gcccattcat ccagcaaatc actggtgagc tggttacgtc
    12661 cggaattata catccccagt gtgcgcgctt tcgccagata gaaatcttcc gacgactgca
    12721 ccaccatcgg gcgcatctga ttagcacggt tgcgggcatc tgccagacga ctttccggca
    12781 acgggtgagt caataaaatt tccggcgggc gcgaggagta acgcgcctga tcgagtaatt
    12841 tttcgaggaa ggttggcatc gcttgcggat cgaatcccga gcgttgcagc acctgaatac
    12901 caatgcggtc cgcttcctgt tcattttgct gggtgaaact gatcatcccc tgacgcgttc
    12961 ccgccagtgt accggtcagc gccgccatcc ccgcctgcgg actggccatc gccagtaaaa
    13021 tagaacctaa cgcgccgacc caggtcagcg gcgcgctgcg ttgttgatct tccatcgctc
    13081 gcgccaggtg acgttgggtg acgtgggaga tttcgtgcgc cataactgaa gccagttgac
    13141 tttcgttatc ggaataacgg aacagggcag agtgcagcac cacgttgccg ccaaagaaag
    13201 caaaggcgtt aatttcgtcg ttgttgatca gaaaaaaatg aaacggtgtc ttaaccgaat
    13261 tggcatgcga aaccagacgc atccccagcg aattaatata ttgcgttaac agcgggtcat
    13321 taattaacgg cgcgctgccg cgtagctggc ggacataata gtcgcccatc tgcatttcct
    13381 gaccaatgga aagcgtgctt cctgcggagg ttcccatatc cggcaaggtg tctgcgctgt
    13441 cggcaaacgc cggggctacc tgaccaatgg tcatagcagc aatgagggtt gcaaccaggt
    13501 tttttttcaa ctgcctgaac ataacctcta tcctgtattt ctgaagatag tcatttgacc
    13561 gatactgtga aatatcgttc ccaccgcgcc gcctttgagt gtaacggcga agattagccc
    13621 actcaaatga cttttcagtt tcctgaatcc gtcataaaaa gcgaagtctt ttttgtgaca
    13681 tttcgataca attgcggatc gcaatcccgc tcttgaggtt cagggaaggt ctttatgctc
    13741 gaaatgttga tgcaatggta tcgccgccgt tttagcgacc cggaagcgat tgccttgctg
    13801 gttattttag ttgccggatt tggcattatc tttttcttta gtggcctgct tgctccgttg
    13861 ctggtggcta ttgtgctggc ctatttgctg gaatggccaa ctgtgcgcct gcaatctatt
    13921 ggctgctccc gccgctgggc gacgtcgatt gtattggtgg tttttgtcgg tatattgcta
    13981 ctgatggcgt tcgtggtact gcctatcgcc tggcaacagg gcatctactt aattcgcgat
    14041 atgccgggga tgctcaataa gctttctgac tttgccgcca cgttgccgcg ccgctatccg
    14101 gcgttaatgg atgcgggcat tattgatgca atggccgaaa atatgcgcag tcggatgctg
    14161 accatgggcg attcggtggt gaaaatttcc ctcgcctcgc tggtcggttt gctgaccata
    14221 gccgtctatc tggtgctggt gccattgatg gtcttcttcc tgctgaaaga caaagagcag
    14281 atgctgaacg ccgttcgtcg ggtgctgccg cgcaaccgtg gactggcagg acaggtgtgg
    14341 aaggagatga atcaacaaat caccaactat atccgcggca aagtgctgga gatgatcgtg
    14401 gtggggatcg ccacctggct ggggttcttg ctctttgggc tgaactattc gctgctgctg
    14461 gcggtgctgg tcggcttctc ggtacttatt ccgtacattg gcgcatttgt ggtgaccatt
    14521 ccggtggttg gcgtggcgct attccagttt ggtgcaggca cggaattctg gagctgcttc
    14581 gcagtgtatc tgattattca ggcgctggac ggcaacctgt tagtaccggt gttgttctcc
    14641 gaagcggtta acctgcatcc gctggtgatt attttatcgg tggtgatctt cggtggtttg
    14701 tggggattct ggggcgtatt cttcgccatt ccattggcga ccctgatcaa agccgtgatc
    14761 cacgcctggc ccgatgggca aatagcgcaa gaataatctt cagcccgacg aactgtttcg
    14821 tcgggctgat ttttcaggat tcctgacgta aataaatagc ccgataacaa atactcacca
    14881 gcactgcacc gccgataata ttacccagca tcaccgggag cagattggcg gtaataaaat
    14941 ggctgaccgt cagagccgga aaattgtctg cgctactgtg cgccagttgc cagaaggggg
    15001 ggaggggcga aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag
    15061 tgctcaaagc cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg
    15121 cataacgaac gcccgcaata actcatccac agcgccaggc aaaccatcag attgcacata
    15181 atgccgaggc tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa
    15241 accgcgactc cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa
    15301 ctgaaacagg caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa
    15361 gttcgccaac taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac
    15421 aggctggtgc cgcaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc
    15481 agatgggtta atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat
    15541 ccaatcgtca taaacacgcc agcggacatc gccattaaaa agaccgatac tttgctgcgg
    15601 gcaattttat gggcagcaat ccgttcagcg attgccgctt ttctggcgct caactgcaag
    15661 tcgaaagaga gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga
    15721 accgagatcc ccagacgctg cattcgtgac agcagcgtag tgcgcttcat ccctaatcgt
    15781 gtcgccgcgc cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg
    15841 cgctgacgtt cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa
    15901 ctgcgaagcg ctgaatcttc atttagcgtc ggcagtaaac ggctttgtcg gacattaaga
    15961 tgtaaattca gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc
    16021 tcgcgcacgt tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggttgggatg
    16081 gcgtcaattg cgcgattcat atggcgcgcc attttttgcg tgaaatgttt tgctaaaaga
    16141 gggatatctt ccggacggtc gcgcagcggc ggcaattcca gtgggaagac attcaggcga
    16201 taaaagagat cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg
    16261 gcggcaatga ctctgacatt caccgggatc gttctactcc cgccgagaca ctcaatctcc
    16321 cgctcctgca atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca
    16381 tcgagaaaca acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca
    16441 ccagtaaacg cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg
    16501 gcagcgcagt tgattttgac cagcggcttg tcgtgtcgcg ggctaagttg atggatcgct
    16561 ctggcgatca cctctttgcc ggttccggtt tcaccacaaa tcaacaccgt actgtcgctc
    16621 ttcgccacaa tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg
    16681 tcaccgacgc ccagatttga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa
    16741 ctttcctgca aatcggtcat gctgcgccag gcatcggcat tgccaacggc aatagcgatg
    16801 cgatcggcta tgtgttgtag tagctggcag ttttcctcac taaagagagt ggaagaggta
    16861 tgcgccagca acaatgcacc cggtgtatgg ttgccaaagg taagcggtat caggagcgca
    16921 gattcgcagc cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga
    16981 tcatctgcac gatgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca
    17041 ctgtcgccag gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac
    17101 cacaggctga acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga
    17161 ccgaaaaaat gatggatctc acgggcgacg tcagcgatca gatcgtcgag atcaagatgt
    17221 gacagcactg cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc
    17281 aaacgcagcg ccacctgttg ttcttcatca ttgagccatg attgcagaac cggggtgatt
    17341 atgcgtgtca gccagttaaa cagtgtgcaa tcctggggag taaaacgact gccgtgggtg
    17401 cgaataaagc gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa
    17461 tcgaccatct cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag
    17521 cggtaataga agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc
    17581 ggcggcagcg ggatgaaggc gcgtaataaa gacgccttgc cgtgtttctg tgctaaccgc
    17641 tccgcgagca ttccgtttac cgcttcaatt gttattcctt gtggcggggc aaacatcgcc
    17701 tcgtctgaca tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc
    17761 tggccatcag atagatagcc ggttccagca caatttcatc caacagatga atgaattgaa
    17821 tgctacgcgc ttgccacaca gacgtcgcgt catctgccag tggcgcgaat gcccgggtca
    17881 acagggcggt gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct
    17941 tacgtcgggc ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc
    18001 agcggaaggc gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt
    18061 aatacattac ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc
    18121 gcagcgtcca gaaaacaatt tccccgcact cttcagtcat gtaaccccct gagtacgcga
    18181 aagagttgct gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta
    18241 acccgatttc ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc
    18301 ccctgacgat agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc
    18361 gggatctgtg gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccacagcg
    18421 tgaatcttct gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt
    18481 gttggcgggc agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag
    18541 acgctgtaga gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacag
    18601 atttatgatc gggggcagac tcatacgccc gaagtgcagg catacgcatc gcccgggtga
    18661 ctgcgccagt aaataacaaa atatcggcgt gacgcggtga tgaaacaacc ttaatgccaa
    18721 aacgttctgc gtcgaatacg ggggtgatgg cagcaaaaat ttcgatttca caggcgttgc
    18781 agccaccgca atcgacgcga taaacgtagg ctgagcgacg gatatcctgt agcaaatgcc
    18841 gcttcatatt ttgcgtttgc tcgtccagcg tgatgggctg gctgacatat tgcgtaagca
    18901 ctggactcat agctgctcct tagccaccag cggtacatct gtatcgtcgt tgagcagcgt
    18961 cgcgcgttgt ttgcactccg ggcagacgct cgcttgcgcc cataacattt cgcggttttg
    19021 tggcgcattt tgttgctgtg ccaacaattc agcagccagt gcgacggttt tttgcggggc
    19081 aaacgggcgt tcgcaacggc tgcaacgttg tagatggaac gtcgcgcggg tatagagatc
    19141 ggctttattg gtgacggtca gttcaaagtt attggtaagc tggatggctc tggtcgggca
    19201 cacttcttca caacgtccgc agtaaataca acgccccaga tagagctgcc aggtgcgcga
    19261 attttgctgg tcgtcggtct ggatagtcag cgcatttgcc ggacaagcac aggcgccgca
    19321 ggcaatacat tggctgggca tcaggtccgg tttcccgcga aagcctgggc tgacctccag
    19381 tggcgcgaag ggatatttca ccgtcgcggt tccggcgcgc ataatagttt tcagtaactt
    19441 cagcatcttc tgtccttact tcagcggcga acggttacga tcaatgccgt agcgttcgat
    19501 ctctttatac ggcacggttt ttgactggcg cttgcgcaca tctaccagcg tcacacggtc
    19561 ggtacaggag tagcagggat caaggctacc gataatcagc ggtgcgtcag aaacggtatt
    19621 gccgcgcagc atgtaacgca acaccggcca gttggcgtag gtggcggcac ggcagcgcca
    19681 gcggaacaat ttttggttat caccgagcat gctccagtgc acgtcttcac cgcgtggcgc
    19741 ttcaacaaag cccagcgcga atgcgtgagg tttatagcta aagccttcgg tcagcagtgg
    19801 ggtatccggc atgttgtcga gggcaaattc cagcattgcc agcgaatcaa acgtctcttt
    19861 gacacggacc atcacgcggg agaaaacatc gccgccggta aaggtaaaca gtgtttttgg
    19921 aatattgccg tagtcggcgt aggggtgatc aaagcgcaaa tcacgggcga tttgtcggtc
    19981 gagaatgcca atgccctgag tgcgttgttc catattcggc gtagcaagca gcatctctac
    20041 cagctccgac acgtcggcgc gcatctcgcg caccagtttc agcgtttgca gacgttgctc
    20101 tttgagaata tcgcggcgaa caccaccaat cagattcaga ccgtaggttt tacgcgaccc
    20161 gatcagcaat tccgccatcg tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc
    20221 ggtatcaaaa ccaacgaaat ggcaggagag gccaaggtta agcaaatgac tgtgtagccg
    20281 ttcgacttcc agcagaatcg agcgaatagt atgtgctcgt tgcggcacct caatccccag
    20341 tgcattttca accgaattgg tataggccac actgtgggca aaaccgcaaa tcccacacac
    20401 gcggtccgat aagaaggtca cttcgttgta gcccatccgc gtttctgcca gtttctccat
    20461 gccgcgatgg acataaaaca ggcggtaatc agcatcgaca atttgctcgc catccacaaa
    20521 caagcggaag tgacccggtt catcggaggt gatatgcagc gggccgacag ggatcacccg
    20581 cgcatcgctg ttgccctcat tgataaacgg atacgtttcg gaatcagtcg tcggttcagg
    20641 gcgcagtcga taatccatcg catctttgcg cagcggatgc atatcttccg gccagtcatc
    20701 gggcaacacc aggcgacgct gatccggcag gccaaccgga atcagcccgt acatatcgcg
    20761 aatttctcgc tcgccccaga ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg
    20821 actgtcggca tcgaccagcg ccttcaccac aatccagcat ttttcggccc cttccattga
    20881 aagggcataa taaaccgcgt aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac
    20941 cggaagccag ccatcatgtt gataataaag atactgtaca acgtcaggca gcagattgat
    21001 tttcaccgta atggtgacct gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg
    21061 gaactgcgtt ttcagggcgg cgagaatcgc ttcgccacga tttgacgatg aattaacgtt
    21121 cactgtgttt ctccgaaaca gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac
    21181 gttgtgcagg cagatcgtgc gtccctgaga ggactatagt ggaagcgccc gccaggatcc
    21241 tgatgacagg ttgtggaata tgcgttccca tcgccagcat catgaccagc agaatcacca
    21301 ttggcgaggt ggtcaaccag ccgagatcac cccggttaac ggcctgcggc ggtttcgcca
    21361 ttaacacccg cgcagccatc cgtaccaggc ccgccagcac cagcgttaac agcaataaca
    21421 gcaggacgat aatcagcagg tgattacgtg ccagtccggc ggtaatggtc ataaattcgc
    21481 taagaaaaat gttgaagggc ggcatccctg ccagcgccag cgcaccgccg ccaaacagca
    21541 cggcggtaaa tggcatgatt ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt
    21601 acttgagcag tacattgccg gaaccgcaga acagcagcgt ttttgccaga ctgtggttta
    21661 agatgtgcag cagcgcggca aaaattccca gcggcccgcc aatgcctagc gccaccgcga
    21721 ccagtcccat gttctccacg ctggagtacg ccagcagacg cttaatgtcc cgctgtacca
    21781 gaatgaaaaa tgccgccacg gcaaccgaca acatgccgaa gatgagcaac aaccggttgg
    21841 ggaaatcgct gccgatggcc tggcaaataa tgatgtaata gcgaatcagc accaacagcg
    21901 cgcagttcag caatacggcg gagagtaggg cgctgaccgg actcggcgct tcactgtgag
    21961 catccggcag ccaggcgtgc atgggaaata gcccggtttt ggtaccaaag ccaattagca
    22021 caaacacaaa ggccaacagc attaatgttg gatcaagcaa ggacgattgc ttaagaacct
    22081 cgctccagaa tatcgccatt tctgcctgcg gcataacgct ggcggcgttg gcgtatacca
    22141 gcacggtacc gaacagacca aaagcgacac caacagtaca aataatgatg tacttccatg
    22201 cagcttccag cgatgaacgc tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg
    22261 tggcttcgat cgccgcccac atcacaatca ggttattgct ggtaacaacc agcagcatgg
    22321 tgaacaaaaa cagatggaag aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc
    22381 cgtgctccac ttcatgacgc atgtagccaa tcgagtaaac accggtgaga aagccaatca
    22441 caccaagaat ggcgaggaac aaaccgccca gaccatcaat atgaagccac agtcccgcag
    22501 cgaatatttc tcctgcatca gcggcagttt ggaccaccca gagtgccaga atcagcagca
    22561 gtgtgatccc taagctatgt aatacggtca ccgtacaagt cgcagagagc ctccgtttcc
    22621 ggcaggcaaa acagagcagc gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca
    22681 cagaataact catctcatta tcccttcagc gcggtcaagt cgttcacgtc cagcgtgccg
    22741 tgggtacgcc atatttttct tgccagtaac accatcacaa tgacggcgaa gatggcgtcg
    22801 gtagcgatac ctatttccac cagttccggt gctcgccagg caagaagcgc cagcaccaga
    22861 tgggagccgt tttccatcag gcagtaacca aaaatttgcc gcaggatatt gcgctggctg
    22921 acaatgcaca gcaggccaag cagaaaatga cctaacgcta ccgccagcgc cggtttcagc
    22981 ccggtagcca tcggtagctt cacgggctga acgacaaatg cgcaaagcag gacaattaac
    23041 gccgcgagca gtgccatcat tgccggaccg aataacgctt tttccgggat gttctgggga
    23101 atatttcgtg cagcgtaagt catgattaac ggtaccagca gcactttggt gataaaggcg
    23161 ctggcggacc agatcagcag ttgctctgcg gcgaacaggc acgagagggt ggcgaaaata
    23221 gacaccagca ccagtgactg gcaggcgtaa aatccgcatg acaggcgata gcttttgacg
    23281 ctaatcacaa acagcgatgt cagcatcatc agtcccgcca gattatttac gatcatagaa
    23341 ccggtcattc atcatctcct tactgcaacc aggtcgcggc gattacgctg gaaatcagcg
    23401 acatcacaat cagtacaatc aacaccaggc gcattgatcc tggcagcggt gcggcatcgg
    23461 cgacggcctc gctcggtttg ccaggcacaa cgcgaccaaa ccagcgaata aaccaggcga
    23521 aactggcgac cgattcaatc atcagcagaa tcagggcggg cagcaggatc cagtactcca
    23581 ctgacaacgc aaaaccggca acaaacagcg ggaatttact gaagaagccg ttgaacggcg
    23641 gtacgccggt aatcgccagc gctaccacgc agaaacccac gcctggcaac ggcagggtgt
    23701 gcaatacgcc acgcagacgc ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg
    23761 ctacaaggaa aaacaggctt ttagcgaacg cgtggttgac gatgtaggcg atgctaccct
    23821 ccagcgccag ccgcgagccg aagatggaga gcgacaagcc gaagaacatc cagccaagtt
    23881 gagtgatggt cgaccaggcc agcaaccgct tcatatcctg ctgtggcaaa tacatcagga
    23941 agccataaag aatggtgacc agcgccatga ccatgccaac gccgccaatc acatgcggga
    24001 tattgccgcc gtcgataatg gcgcgggcaa aaatgtaaac gcccactttc accatcgatg
    24061 cggcgtggag ataagcgctg atcggtgttg gcgcttccat tgcatccggt agccacgctt
    24121 gcatcggtag ctgggccgat ttcccccacg cggcaaacag gatgccgcca taaaccagat
    24181 aacgtgcgtc gccgtgtaac tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa
    24241 acagcgtggc ggcggcaaga tacaacccca acgaaccgat atgggtgata agcaacgctt
    24301 ttagtgctga acgctgcgct ttatcgctct gataataact gatcaacgcc caggagcagc
    24361 cgcccgtaat ttcaaaaaac aacaactgac cgagcagcgt cgaggagagt accagtcccg
    24421 ccatcgcgcc gataaacacc agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt
    24481 gttcgcgatt tttatccgtc agataacccg tggagtagat cgtgaccagc aaaccgagaa
    24541 acaccaccac aaacagaatc agcgtactca cgcgatcaat gaccaggcca aacaatgcca
    24601 catcccccac gttgaccaac gtcaacgtga cggcaacttt atcggcctga taaaacgcgg
    24661 agatcagcga caacatgcac agcgtggtca gcgcggcgaa caaaaccccc cattcggcgg
    24721 cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat aaaaggcagc attaacgtcg
    24781 tcagagcaag attttccata ttccgtcagt gctccttaca gaccggttaa ccagaagacc
    24841 catgcaagcg cagcaaggct gaagccaagc caggtcacat ggtgaatgag taaaaaacgc
    24901 ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa aaatcagcaa aaccttgagc
    24961 agcgtaacga caagtgaagt cagcaggcag gcgagagaaa gttcttgcgc gcgcccaaag
    25021 ggcagaaaca gggccacaaa cagtgctgcc atcacgacct gtttcagccc cagcccccat
    25081 ttcgccagcg ccagcccggc accggaatat tctgtcagcg ggccttcctg taattcctgt
    25141 tctgcttcag caacatcaaa gggaattttt cccatctcaa tgaagcaggc aaaaccacag
    25201 gccagtaacg ccagtacggt ggttagcggc gagttccagc ccatcgccag cgtattgctg
    25261 atcatctcga tatgcgtgga atccgctatc agtgccaata ccagcagtga gagaataagc
    25321 atgggttcga ccagaatgcc gagcgtcaac tcgcgactgg caccgactcc cgcaaacgga
    25381 cttccggtat ccagcccgga aagagcaaag aaaaaacgaa acagggcaag aagatagata
    25441 agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg taataaatag tggtagggcc
    25501 atcgccagca ccagcatgct gctgattaat acccagggca tcaggcggaa catcagacct
    25561 gaagatgtcg gcgcaacttc ctggcgttta aacagtttgt ggatatcgcg ataatcctgc
    25621 cagatccccg gttcgcggcg ggagtgcatt cgcgcgcgta tctgccggga aatacccgta
    25681 aaaagtgggg ttagcatcag cagaatcacg gcctgtgcta acgcaaaaat gaccagatat
    25741 ccgtcgcaaa gagtttgtct catggtgatt tccttagacg gcaatagcga ttagcagcac
    25801 aaccagtgcg gcgaccacat acaggcaata gagacgaaag tcgccgcttt gcagatgctg
    25861 tatttctttg gccagccgtt gggtggcgct cacgatgggg cggatcaccc gctcatccca
    25921 gaagggttct gtgctctgag ccctggcggt gacgtgcgca agacctttat ttagcctcag
    25981 cgtagggtcg agctgttgtc gtagacgaaa tagcgcagag aagaccacac gcagcggctg
    26041 catcacgcca ttgcctgacg gggccatcgc attttcccag ccgtagccgc atgcccaggc
    26101 atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac cacagccagt aaaggccagg
    26161 cagcaaaggc atcgccagta gtaacagcaa cagtaatgag gaggttacct gtgtatgaaa
    26221 cgtgccgggt acaagagcta ttccgctggc gacagtgacg ggaggggtat tggtaaacgc
    26281 atgggcaata tgcattatct tcggtgccag ccaactggca gtaagcgcaa ttaatacgca
    26341 gagtgccgcg agcagtagca tggcgacgat catcgtattt ggcacttcct gtgcctcttc
    26401 agcgtgtgta ctgcgcggcg caccacagaa agtaataccg tacattttga cgaagcacat
    26461 tactgccagc ccaccagtga ctgccagcat taccatagca ataggacccg caagttgtag
    26521 cgctacggct tccacacgac ttagtgagaa cagcgactgc caggtgtacc attcgctgat
    26581 aaaaccattc agcggaggaa tggctgatat cgcgaggcaa ccaatcaggc atgctgcggc
    26641 tgtccacggc atccgtttcg ccagtgcccc cattttttcc atgtcgtggg tatgcaaacg
    26701 cgaaataatc gcgcccgcgc cgagaaatag cagccctttg aacagcgcat ggttgagcag
    26761 atgaaacagt gcgccgagca gtccaaccac ggtgagcagc gggtcgtgca gtgacagacc
    26821 gtccatcgcc acaccgactg ccagcaaaat aatgccgacg ttttcgacgg tactccaggc
    26881 cagcagccgt ttgatatcct gttccgccag cgcatatagc acgcccagga gcgcggagat
    26941 tgcgccgatc gccatcacca gaatgcccca ccacagaggc aaacccgttt gcgccagcag
    27001 atccatcgct actttcagga tgccgaaaat accgattttg accattacgc cagacatcaa
    27061 cgccgaagcg tgcgatggtg cggcagggtg agcgcgcggc aaccagctgt gcaacggtat
    27121 catcccggct ttcgcgccaa aaccgaaaaa ggccagcaga aacaccgccg acgccagccc
    27181 cggagaaagt gaaagcgtgc gaaactggca aaatcgaggc tgccgctttc gcgccccatc
    27241 agcaagaagg cgatcataat cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg
    27301 ccagcgttga tcgacgtttt atcctgcctg gcaatgacca gaaaccagga agacagcgac
    27361 atcatttcaa acagcacgat gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc
    27421 gatgcgatga aaagattcat aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg
    27481 cgcatataag tcaatgaata gagcgaacaa accaccacca gcaaggagat aaccagcacc
    27541 ataaatgccg agaggctatc cattcgcagg gttaaatcgg caaagtcaaa aggggtggca
    27601 aaatgggcga ctaaagttac accagcatgt aattgcgtaa ttccgctgat gatgccaatc
    27661 acgccgccca ccagcgatgt gatgccggaa agcttaatag ccagtctgtc cagaccgagt
    27721 aaaaacagcg aagccagact ggcaaacaga tagaggatca gcgaccaggt taataattgc
    27781 agggcatcca ttagcgttgc tcctgagtga gggaaagggg atcctccccg ccattggcaa
    27841 accagcttgc ggcaaggcgc tgtttttctt tcatctgacg ttgcaggcta tcgtcggtga
    27901 tcagccgtaa cgcctgattc gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat
    27961 cacacaggtc gcatttcacc gcgacggtct ggacaccagg ttcccagcgc agcaacgcat
    28021 gttggggcag cacgttttct tcgccgtttt tgagtgagcc ttcagcctga aaaacatatt
    28081 gcgcatgggc attcaccgga cggcttcctg aagcgctgat tgcgccaaat gggcagacca
    28141 cggcgcaaag cttgcagcca atacagaggc tttcgttgag ttggatcgca tcatccctct
    28201 gagagatggc attgaccggg cagacctgca ggcaaggggc ttcctcacag tgatgacaca
    28261 cgacaggggc agtgattgtt gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac
    28321 cttgcgtttt atgcacgtcc gaacaggcag cgagacaggt attacatcct gtacaccaca
    28381 gtggttcggc caccacaaag cggttcatgt cggctccttg aaaatgatat gaaacattgc
    28441 atttcatatt gcataaagga tgccattctg attaactcaa tatattcaat aagttatgta
    28501 ttaactggat tctgaacggt acaaaaatga tggtcattaa cgttcagcaa aaaatggatt
    28561 cgacacccgt gtcgcagggg aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg
    28621 ctggccgcaa gcgcagccag cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc
    28681 agttcagcac aacgtcgtgg tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt
    28741 tgccatcagc gtcaatcagg aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga
    28801 aggacttttc accccagacg ccgaattgtt cgcacacctg gtggtcctca tcagacagga
    28861 gcgtaaagtt aagcagctct ttttccgcaa aacgggagag tttttcgggt ttatcggtgc
    28921 tgatacccag cacatcaacg cccgcttttt tcaactcatc catgttatcg cgtaagccgc
    28981 aggcctgtac ggtacagccg ggggtcatgg ctttcgggta gaaataaacc agaacacgct
    29041 gtccctggaa gtcggtcaaa ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg
    29101 gtgcgatatc accggctttc agtggattca ttacttaact ccatcctgtt catcatgttg
    29161 ggaataattg acgacgttaa tactgccttg tgcattgagt tctgtacata gggctttgaa
    29221 cgcttgctca atatttgctg cgtccgcaga tgcggggctg tgggcggtta tctgaatatg
    29281 caactgcgca gcccgttcat tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt
    29341 catatgatgc gcgtcgaaaa gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac
    29401 atcgacctga acccagacag atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt
    29461 catcacgatt aaaagatcca gttcggcacc tttcaacggt aacgttgatt caatcagagt
    29521 aatggcattc catgaaccgg aaagcagcat aataaacgtg aactcttctc ccagcatcgc
    29581 caggcgactg tcttcaatat tacagccgca actactgaca tgacgggtga tggtgttcac
    29641 aattccaggg cgatcggcac ccaacgcagt gatcaccaga taatgttgcg atgacagtgt
    29701 caaactggtt attcctttaa ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc
    29761 atacaacaat cagaacggtt ctgtctgctt gcttttaatg ccataccaaa cgtaccattg
    29821 agacacttgt ttgcacagag gatggcccat gttcacggga agtattgtcg cgattgttac
    29881 tccgatggat gaaaaaggta atgtctgtcg ggctagcttg aaaaaactga ttgattatca
    29941 tgtcgccagc ggtacttcgg cgatcgtttc tgttggcacc actggcgagt ccgctacctt
    30001 aaatcatgac gaacatgctg atgtggtgat gatgacgctg gatctggctg atgggcgcat
    30061 tccggtaatt gccgggaccg gcgctaacgc tactgcggaa gccattagcc tgacgcagcg
    30121 cttcaatgac agtggtatcg tcggctgcct gacggtaacc ccttactaca atcgtccgtc
    30181 gcaagaaggt ttgtatcagc atttcaaagc catcgctgag catactgacc tgccgcaaat
    30241 tctgtataat gtgccgtccc gtactggctg cgatctgctc ccggaaacgg tgggccgtct
    30301 ggcgaaagta aaaaatatta tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa
    30361 ccagatcaaa gagctggttt cagatgattt tgttctgctg agcggcgatg atgcgagcgc
    30421 gctggacttc atgcaattgg gcggtcatgg ggttatttcc gttacggcta acgtcgcagc
    30481 gcgtgatatg gcccagatgt gcaaactggc agcagaaggg cattttgccg aggcacgcgt
    30541 tattaatcag cgtctgatgc cattacacaa caaactattt gtcgaaccca atccaatccc
    30601 ggtgaaatgg gcatgtaagg aactgggtct tgtggcgacc gatacgctgc gcctgccaat
    30661 gacaccaatc accgacagtg gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt
    30721 gctgtaaagt ttagggagat ttgatggctt actctgttca aaagtcgcgc ctggcaaagg
    30781 ttgcgggtgt ttcgcttgtt ttattactcg ctgcctgtag ttctgactca cgctataagc
    30841 gtcaggtcag tggtgatgaa gcctacctgg aagcggcacc gcttgcggag cttcatgccc
    30901 cggctggaat gattttgccg gtgacctccg gtgattatgc aatcccggtg accaacggta
    30961 gtggtgctgt cggtaaggcg ctggacattc gtccaccagc ccagccgctg gcactggttt
    31021 ctggcgcgcg tacccagttc acgggcgata ccgcttcatt gctggtggaa aatggtcgtg
    31081 gcaatactct gtggccgcag gtggttagcg tgctgcaggc gaaaaactac accatcaccc
    31141 aacgtgatga tgctggtcag acactgacca ccgattgggt acaatggaac cgtctggacg
    31201 aagacgagca gtatcgtggt cgttatcaaa tctctgttaa gccgcagggt tatcagcagg
    31261 cggttacggt taaactgctg aacctggaac aggcgggcaa accggttgca gacgcggctt
    31321 ccatgcagcg ttacagcacg gagatgatga acgttatttc cgccggtctg gataaatctg
    31381 ccactgacgc cgcgaacgct gcgcaaaatc gtgcctccac cactatggac gtacaaagtg
    31441 cagctgatga caccggttta ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc
    31501 aacgtctgcc agcggcgctg gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc
    31561 agggcaacat ggccgtaact tataagccgc tgtctgacag cgactggcag gaactgggcg
    31621 cgagcgatcc aggcctggca tccggtgact ataaactgca ggttggcgat ttagataacc
    31681 gcagcagcct acagttcatc gatccgaaag gtcatactct gactcagagt cagaacgacg
    31741 cgctggtagc tgtcttccag gctgcgttta gcaagtaaaa atacagggct ggaatcatcc
    31801 ggcccttttt tctgatatga tacgcaaacg tgtgcgtctg caggaaaacg cgattttagc
    31861 ggtaattcgc acgaaatttg tttgtcggac gtagttcgga taaggcgttc acgccgcatc
    31921 cgacaaaaca tccggcacac cagacagcaa aagattttaa aacgttaatt cacacccagg
    31981 agtgataaag atgcaaaagc aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag
    32041 cacggaaaac ccggacctgt tggtgctcga attccgcaat gatacgtcag caggggatgg
    32101 cgcgcgcatt gagcagtttg atcgcaaagg tatggtgaac aacaagttca actacttcat
    32161 tatgagcaaa ctggctgaag cgggtatccc gactcaaatg gagcgtctgc tctccgatac
    32221 cgaatgtctg gtgaaaaagc tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc
    32281 tgctggctct ctggtgaaac gtcttggaat cgaagaaggt attgagctga acccgccgct
    32341 gttcgatctg ttcctgaaaa acgacgccat gcacgatccg atggtcaacg aatcttactg
    32401 cgaaaccttt ggctgggtga gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa
    32461 agcgaacgac gtgctgaaaa aactgttcga tgatgctggt ctgattctgg tcgacttcaa
    32521 gctggaattt ggtctgtaca aaggcgaagt ggtactgggt gatgagttct ccccggacgg
    32581 tagccgcctg tgggacaaag aaacgctgga gaaaatggac aaagaccgtt tccgccagag
    32641 cctcggtggc ctgatcgaag cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga
    32701 ctgatttttc tgttcatcat cttgccgtgc agctggcacg gcaagacaac cgctctcgga
    32761 atatatgcat tgaacgctca ttcctccgcc atttcatccc gctttcctgt ggtaatcttc
    32821 ctctgaacca cctacgatca tttctataat gaatatattg ttgaggtatc tatgcgttgg
    32881 caagggcgac gtgaaagtga caatgttgaa gacaggcgca acagctctgg tggtccatct
    32941 atgggcggtc ccggttttcg cctgccaagc ggtaaaggcg ggctgatttt actgatagtc
    33001 gtgctggttg caggctacta tggtgttgat ttaaccgggt tgatgaccgg gcagccggtt
    33061 tcccaacaac aatcaacgcg gtcaattagc ccaaatgaag acgaagccgc aaaattcacc
    33121 tcggtgattc tggcaaccac ggaagacacc tggggacaac agttcgagaa gatgggtaag
    33181 acctatcagc aaccgaagct ggtcatgtac cgtggaatga cgcgtaccgg ctgcggggcg
    33241 ggccagtcca taatggggcc gttctattgc ccggcggatg gcacggttta tatcgatctc
    33301 tccttctatg atgacatgaa agacaaactt ggcgcggatg gcgattttgc ccaggggtac
    33361 gttatcgccc atgaagtcgg tcatcatgtg cagaaactgt taggcatcga gccgaaagtt
    33421 cgtcaactgc aacaaaacgc gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa
    33481 ctccaggccg actgttttgc cggtgtctgg gggcatagta tgcagcagca aggcgttctg
    33541 gaaaccggcg atctggaaga ggcgctgaac gcggcgcagg ccatcggcga tgaccgttta
    33601 caacagcaaa gtcaggggcg agtagtacca gacagtttca ctcatggcac ttctcagcaa
    33661 cgctacagct ggtttaaacg tggtttcgac agcggcgatc cggcacaatg caatactttt
    33721 ggtaaaagca tttaacttcc ggggcaggga tggctgaact gactgcgctt cacacattaa
    33781 cagcgcaaat gaaacgtgaa gggatccgcc gcttgctggt gttgagcggg gaagagggtt
    33841 ggtgttttga gcatactctt aagttgcgtg atgccttacc tggcgactgg ctgtggattt
    33901 cgccgcggcc agatgctgaa aaccactgtt ctccctcggc actacaaact ttacttgggc
    33961 gcgagttccg gcatgcggta ttcgacgccc gccacggctt tgatgccgct gcctttgccg
    34021 cacttagcgg aacgttgaaa gcgggaagct ggctggtttt gttactccct gtatgggaag
    34081 agtgggaaaa ccaacctgat gccgactcgc tgcgctggag tgattgccct gaccctattg
    34141 cgacgccgca ttttgtccag catctcaaac gcgtacttac ggcggataac gaggctatcc
    34201 tctggcggca aaaccagcca ttctcgttgg cgcattttac tccccgtact gactggtacc
    34261 ccgcgactgg cgcaccacaa ccagaacaac agcaactctt aaagcagcta atgaccatgc
    34321 cgccgggcgt ggcagcggta acggctgcgc gtgggcgcgg taagtcggcg ttggcagggc
    34381 aactcatttc tcgtattgcg ggcagagcga ttgtcaccgc gcccgcaaaa gcgtcaacgg
    34441 atgtactggc acaatttgcg ggcgagaagt ttcgctttat tgcgccggat gccttgttag
    34501 ccagcgatga gcaagccgac tggctggtgg tcgatgaagc cgcagccata cctgcgccat
    34561 tgttgcatca actggtatcg cgttttcctc gaacgttgtt aaccactacg gtgcagggct
    34621 acgaaggcac cggacgtggt tttttgctga aattttgcgc tcgctttccg catttacacc
    34681 gttttgaact gcaacagccg atccgctggg cgcagggatg cccgctggaa aaaatggtca
    34741 gcgaggcact ggtttttgac gatgaaaact tcacccatac accacaaggc aatattgtca
    34801 tttccgcatt tgaacagacg ttatggcaaa gcgatccaga aacgccgtta aaggtttatc
    34861 agctcttgtc tggtgcgcac tatcggactt cgccgctgga tttacgccgg atgatggatg
    34921 caccagggca acatttttta caggcggctg gcgaaaacga gattgccggg gcgctgtggc
    34981 tggtggatga gggtggatta tctcaacaac tcagtcaggc ggtatgggca ggttttcgtc
    35041 gcccgcgggg taatctggtg gcccagtcgc tggcggcgca cggcaacaat ccactggcgg
    35101 cgacattgcg tggacggcgg gtcagccgga tagcagttca tccggctcgt cagcgggaag
    35161 gcacagggcg gcaacttatt gctggtgctt tgcaatatac gcaagacctc gactatcttt
    35221 cggtgagttt tggttacacc ggggagttat ggcgtttctg gcaacgctgc ggttttgtgc
    35281 tggtgcggat gggtaatcat cgggaagcca gcagcggttg ctatacggcg atggcgctgt
    35341 taccgatgag tgatgcgggt aaacagctgg ctgaacgtga gcattaccgt ttacgtcgcg
    35401 atgcgcaagc tctcgcgcag tggaatggcg aaacgcttcc tgttgatcca ctaaacgatg
    35461 ccgtcctttc tgacgacgac tggcttgaac tggccggttt tgctttcgct catcgtccgc
    35521 tattaacgtc gttaggttgc ttattgcgtc tgttacaaac cagtgaactg gcattaccgg
    35581 cgctgcgtgg gcgtttacag aaaaacgcca gtgatgcgca gttatgtacc acacttaaac
    35641 tttcaggccg caagatgtta ctggtccgtc agcgggaaga ggccgcgcag gcgctgttcg
    35701 cacttaatga tgttcgcact gagcgtctgc gcgatcgcat aacgcaatgg caattatttc
    35761 actgactcct tcaattttat ctcatggtca ttgtgagtca gcggcgtaaa attaatgaca
    35821 tcagttaagg agttcgccat gaaacatgac cattttgttg ttcaaagccc ggataaacct
    35881 gcgcaacagt tgttactgct tttccacggt gtcggggata acccggtggc aatgggggaa
    35941 ataggcaact ggtttgcacc gctgtttcct gatgcgctgg tggtgagtgt cggtggcgcg
    36001 gagccgagcg gtaatccggc ggggcgtcag tggttttcgg tgcagggtat tacggaagat
    36061 aatcgccagg cgcgtgtgga tgcgatcatg ccgacgttta ttgaaacggt gcgctactgg
    36121 cagaaacaga gcggagtagg ggctaacgcc acggcgctca tcggtttctc tcaaggcgcg
    36181 attatggtgc tggagagcat taaagccgaa ccgggccttg cttcacgcgt cattgctttt
    36241 aacggacgct atgcgagcct gccggaaact gcgtctacag ccacgacaat tcacctgatt
    36301 catggcggtg aagatccggt tatcgatctg gcgcatgcgg ttgcggcgca ggaagcgtta
    36361 atcagtgccg gtggtgacgt gacgctggat atcgtggagg atttaggcca tgcgatcgac
    36421 aatcgcagta tgcaatttgc cctggatcac ttgcgttata ccattccgaa acattatttc
    36481 gacgaagcgc taagtggcgg taagcctggc gatgatgatg tgattgaaat gatgtaaaag
    36541 acagattacc tggcctgata agacgcgtga gcgtcgcatc aggcatcgtt caccattgcc
    36601 ggatgcggcg ttaacgcctt atccggccta cggttgagca ctcagcttac ttcttcggtt
    36661 gatccttttt cggccagtcg tcgtcatcgt cccacttatc gttgaaatca cgatgcgggg
    36721 gaagctccgg cttgttggcg agaaatttct tatggtcgac gcgcttgaga tctttaatca
    36781 cgttcagcag gacgcctacc aaaaagacaa tcaccagaat ccaccaatat ttagccagcc
    36841 agtccatgct tatttcctct tgcagaacca ctcatcaggc gacgagctgt tccatgatac
    36901 gttgatacat acgggcaagt agctgcaggt cggcagcgtt cacacattca ttaattttat
    36961 gaatagtggc attgaccggc ccgagttcca ccacctgcgc ccccatgcgg gcaataaagc
    37021 gcccgtcgga cgttccgcct gtggtcagta gctgcggttt aatttcatta tagtgctcaa
    37081 ccgcgttaac gaccgcatcc accagtttac cgcgcgcggt caaaaatggc tgcccggaaa
    37141 gccaccaatc caccgtatag cgcagttgat gtttttcaag cagggcaagc acctgcgctt
    37201 tgatcatctc atcagtcagt tcggtgctga agcggaagtt aaactgcaca aacagttcac
    37261 ccggaataac gttgttactg cccgttcccg cctgaatatt ggcaatctgc atactggtcg
    37321 ccgggaagaa ttcattgccc tgatcccact caatagccac taattcatta aggaaaggtg
    37381 ctgcgcgatg taccggattg tcagccagat gtgggtaggc aacatgcccc tgaacgccat
    37441 gaatggtaag gttgcaggtt aatgatccgc gacgaccatt tttcaccaca tcacctacca
    37501 cttcgatact cgacggttcg ccaaccaggc agtaatcgag acgctcatta cgtgccatta
    37561 acgcttcgac gacttttacc gtaccgttgt gggcactggc ttcttcatca gaggtgatca
    37621 gaaatgccag tcgccccgta tggttgggat gttgtgcgac aaaacgttct gccgccacca
    37681 ccatcgccgc cagcgagcct ttcatatctg ccgcaccgcg cccgaataac atgccgtcac
    37741 gaatggtggg ttcaaacggg ggattgatcc aacgatcggc gtcgccaggc ggcaccacgt
    37801 cggtatgccc ggcaaaggct aacgtttcac cctgcccacg ccatgcccaa aaattctgcg
    37861 tatcggcaaa gtccatgcgt tcaacggtaa aaccgatcgc ctgcaaacgt tcaatcaaca
    37921 aagcctggca tcctgcatca tcaggactca gggaagggcg gcgaataagc tgttgtgtca
    37981 gctcaataac cgggcacgac atagactaca cctcatggaa aaattgctga taactggaat
    38041 cactgaaacc cagcagcata ggcttaccgg gcacgcagag caatggacgt ttgataattg
    38101 caggcatttc agtcattaat gccgccgcag aggccgcatc ggtgatttta ttgcgggtgg
    38161 tttcgtccag tttacgccag gttgtaccac gggtgttgag taacgcttcc cagcctaatt
    38221 cgttgataaa atcgttcaat aattcgctgt ccagcccatc gacgcggtaa tcatgaaaac
    38281 gatagtcgat gttattggct tccagccaac ggcgagcctt tttaatggtg tcacaatttt
    38341 tgataccgta aagtgtaacc ataatgaatc ctttttcgat ttatgagaat aaatactcat
    38401 ttaagggtga aaacattttg cctgcaaatg attatcagga aatataacat tatcgaagta
    38461 t
//
LOCUS       DBBGLQ010000042        38421 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.042, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000042 DBBGLQ010000000
VERSION     DBBGLQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.042"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(1..788)
                     /locus_tag="ACIYSB_002779"
                     /pseudo
     CDS             complement(1..788)
                     /locus_tag="ACIYSB_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(1017..1937)
                     /gene="malM"
                     /locus_tag="ACIYSB_002780"
     CDS             complement(1017..1937)
                     /gene="malM"
                     /locus_tag="ACIYSB_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGC5429797.1"
                     /translation="MKMNKSLIVLCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(2180..3520)
                     /gene="lamB"
                     /locus_tag="ACIYSB_002781"
     CDS             complement(2180..3520)
                     /gene="lamB"
                     /locus_tag="ACIYSB_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGC5429798.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNADNNANFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3592..4707)
                     /gene="malK"
                     /locus_tag="ACIYSB_002782"
     CDS             complement(3592..4707)
                     /gene="malK"
                     /locus_tag="ACIYSB_002782"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGC5429799.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5072..6262
                     /gene="malE"
                     /locus_tag="ACIYSB_002783"
     CDS             5072..6262
                     /gene="malE"
                     /locus_tag="ACIYSB_002783"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGC5429800.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6416..7960
                     /gene="malF"
                     /locus_tag="ACIYSB_002784"
     CDS             6416..7960
                     /gene="malF"
                     /locus_tag="ACIYSB_002784"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGC5429801.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7975..8865
                     /gene="malG"
                     /locus_tag="ACIYSB_002785"
     CDS             7975..8865
                     /gene="malG"
                     /locus_tag="ACIYSB_002785"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGC5429802.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            9237..10712
                     /gene="xylE"
                     /locus_tag="ACIYSB_002786"
     CDS             9237..10712
                     /gene="xylE"
                     /locus_tag="ACIYSB_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGC5429803.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10756..11166)
                     /gene="psiE"
                     /locus_tag="ACIYSB_002787"
     CDS             complement(10756..11166)
                     /gene="psiE"
                     /locus_tag="ACIYSB_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGC5429804.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(11292..11435)
                     /locus_tag="ACIYSB_002788"
     CDS             complement(11292..11435)
                     /locus_tag="ACIYSB_002788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429805.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            11436..11714
                     /locus_tag="ACIYSB_002789"
     CDS             11436..11714
                     /locus_tag="ACIYSB_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429806.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11761..13857)
                     /locus_tag="ACIYSB_002790"
     CDS             complement(11761..13857)
                     /locus_tag="ACIYSB_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGC5429807.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQYSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13857..14594)
                     /locus_tag="ACIYSB_002791"
     CDS             complement(13857..14594)
                     /locus_tag="ACIYSB_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGC5429808.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14591..15229)
                     /locus_tag="ACIYSB_002792"
     CDS             complement(14591..15229)
                     /locus_tag="ACIYSB_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGC5429809.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGASWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15343..15585)
                     /gene="yjbE"
                     /locus_tag="ACIYSB_002793"
     CDS             complement(15343..15585)
                     /gene="yjbE"
                     /locus_tag="ACIYSB_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGC5429810.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(16084..17733)
                     /gene="pgi"
                     /locus_tag="ACIYSB_002794"
     CDS             complement(16084..17733)
                     /gene="pgi"
                     /locus_tag="ACIYSB_002794"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGC5429811.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      17970..18166
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            18258..19607
                     /gene="lysC"
                     /locus_tag="ACIYSB_002795"
     CDS             18258..19607
                     /gene="lysC"
                     /locus_tag="ACIYSB_002795"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGC5429812.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19860..20132
                     /locus_tag="ACIYSB_002796"
     CDS             19860..20132
                     /locus_tag="ACIYSB_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGC5429813.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     DYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(20265..21137)
                     /gene="rluF"
                     /locus_tag="ACIYSB_002797"
     CDS             complement(20265..21137)
                     /gene="rluF"
                     /locus_tag="ACIYSB_002797"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGC5429814.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITEEFIRGMSAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21349..22038
                     /gene="pepE"
                     /locus_tag="ACIYSB_002798"
     CDS             21349..22038
                     /gene="pepE"
                     /locus_tag="ACIYSB_002798"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGC5429815.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(22129..23760)
                     /locus_tag="ACIYSB_002799"
     CDS             complement(22129..23760)
                     /locus_tag="ACIYSB_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGC5429816.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(23980..27663)
                     /gene="metH"
                     /locus_tag="ACIYSB_002800"
     CDS             complement(23980..27663)
                     /gene="metH"
                     /locus_tag="ACIYSB_002800"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGC5429817.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            27863..28687
                     /gene="iclR"
                     /locus_tag="ACIYSB_002801"
     CDS             27863..28687
                     /gene="iclR"
                     /locus_tag="ACIYSB_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGC5429818.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            29004..31190
                     /gene="espL4"
                     /locus_tag="ACIYSB_002802"
     CDS             29004..31190
                     /gene="espL4"
                     /locus_tag="ACIYSB_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HGC5429819.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPI
                     FEKYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRCVAACGDFGL
                     VMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKENIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDE
                     ITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAINY
                     GNADIVETIFNSLSETGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSIL
                     NALPKLAATHHLDNEQVYKFLSAKNRTSSHVLYHVMANGDADMLKIVLNALPLLIRTC
                     HLTKEQVLDLLKAKDFYGCPRLYLAMQNGHSDIVKVILEALPSLAQEINISASDIVDL
                     LTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYP
                     GLFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGH
                     RVIAELILNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(31159..32895)
                     /gene="aceK"
                     /locus_tag="ACIYSB_002803"
     CDS             complement(31159..32895)
                     /gene="aceK"
                     /locus_tag="ACIYSB_002803"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGC5429820.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(33176..34480)
                     /gene="aceA"
                     /locus_tag="ACIYSB_002804"
     CDS             complement(33176..34480)
                     /gene="aceA"
                     /locus_tag="ACIYSB_002804"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGC5429821.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(34510..36111)
                     /gene="aceB"
                     /locus_tag="ACIYSB_002805"
     CDS             complement(34510..36111)
                     /gene="aceB"
                     /locus_tag="ACIYSB_002805"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGC5429822.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRYADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(36380..37309)
                     /gene="metA"
                     /locus_tag="ACIYSB_002806"
     CDS             complement(36380..37309)
                     /gene="metA"
                     /locus_tag="ACIYSB_002806"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGC5429823.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQDQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            37466..37909
                     /locus_tag="ACIYSB_002807"
     CDS             37466..37909
                     /locus_tag="ACIYSB_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGC5429824.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRTELEDLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRVLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGA"
     gene            complement(37972..38355)
                     /locus_tag="ACIYSB_002808"
     CDS             complement(37972..38355)
                     /locus_tag="ACIYSB_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaA family stress response protein"
                     /protein_id="HGC5429825.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSAVLSDSAVMAMLKQK"
BASE COUNT         9300 a         9879 c         9633 g         9609 t
ORIGIN      
        1 taatcaatag ttaattccaa ttgttctgta atgcaatctt taaaattccc tgtcgacatt
       61 cgcacataat caaacataac agctgtcaaa ttcgatttaa taaatgatat ttctttggca
      121 gacagaaact ctgctctggt gaagtgcgaa gcgtaaagat aacaattatc gaagtttgaa
      181 tttctaatct gacattcgcg aaaattacta tgaccaagtt gactctgcga gaagtcaact
      241 gtatcaagaa tatttttgct gaaatcgaca cattcaccac tcgctttgat aaaaatcaca
      301 ttgtgtaaac gtcgacatct aaatatgaca ttatcaacta ttgcagtagt aaaattgcaa
      361 tccccaattt ctttacattc gaatgttaca tgttgtagat ttgatgataa aaaagacacc
      421 ttcgatataa cgtcaccttt aaaatgacaa cttgttaaat ccaaatcaga caggtcgata
      481 tacccctgcg catctttatc catcagaaaa ttgcaagcgc attcaatttt tttcatttct
      541 gtagaatttt acacttatag tatcattact gattgagact tcacgaaaca tatttattgc
      601 atgatttttt cgttccgttg ttttatattt gaaaaaatat tgctgatata aataaagtta
      661 tctctgttta aacctggcac cccatttaaa ttacgtagta aatcaagatc tactatatta
      721 tttccttgca ccacatcatg tttcatcaaa gagttattgc cgccagaatt taggatatga
      781 cttatcataa aacctctgca taacagaaat aaaataacct ggaaaagtag cctgaatgtc
      841 cagacactac cggcgaaagg aaaagaatct ctgataaggc attgagataa tggatattct
      901 tagtgaagaa gaagtgagtt actataaaaa atcgctcata tcccaggaag gaagcataaa
      961 aaaaggtgcg ccaggagacg caccagttgt tgcaaaatca gcactgtggg gcgtaattac
     1021 cccttgcctt ttacactgct gataaaggtg gaacgggcag atgtcgatcc caggcgttca
     1081 gcttcatcaa gcagttttaa cgccttatca acatcacctt tcgcgacagc gtttttgatc
     1141 gcggtattaa aataactttc cgtgtcgttg agcatcggct cgcttttctt caccggtgcc
     1201 ggagcgggtg cagccacagc tggtgccgcc gtgttaccta ccgtaaccgg agctggagcg
     1261 gaagaaccaa ataaaggtcc taccaacacg ctggagctgg agttcgtttt cactttcagt
     1321 ttcagtaagc catcggtggt atgacgagca accggatcgg ggatatccgg gatcgagtta
     1381 ccgacgccct tggcataggc tttagccggg tcgagcagtt gggtcgtctg ctggagatct
     1441 ttttccgtgg taaagaccag aacataaagt ttttgctgcc ccaacgccgg tgtcaggcgc
     1501 ataacgcctt ccagccgatc tgcactcatc acgcctggtt cctggtaggt gaaataactg
     1561 ctggggaaga aggctgatgg ggtcatgttc tgatcaagaa tcagcacgtt cggcgcaaaa
     1621 acgctggttt gtttgttcac ttcgctggtc agcgtcaggg tcagttcgcc aatgtttgcc
     1681 gggacgctgt acgcagcaac cggaccactg atgccgggaa cgttcagttg ttggccgccg
     1741 gtcgccagtt gggtggtctg ggttttagat tgatcgaccg gtgtccaggt gagttgttgc
     1801 agcgcagcag atggaatggc tggcgcgtcg ctggtgtttt gcggtacgta gttaacatcg
     1861 gcaaggctaa ttccaggcgc gcttgccagt aaccctgctg ataaacagag gacgatgaga
     1921 cttttattca ttttcattgt tttcacctca aaatctggag ctcagcggta gccaggcaat
     1981 agcgcgctaa acccgataat cagaggggct ttcgcccctt tcagataatg acaacctgtt
     2041 tttatgccgg atgcggcgta aacgccttat ccggcctaca tttgacagcc gttgtaggcc
     2101 tgataagacg cgcaagcgtc gcatcaggcg ttggttgccg aatgcggcgt aaacgcctta
     2161 tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag gtccactcgt
     2221 cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct ttgccgaagt
     2281 tcgcgttgtt atcagcgtta ccggtgtagt cgtaacccca tttctcatcc cacttggcgt
     2341 aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc tgccattgtt
     2401 gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat tcgacgttgt
     2461 cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc gggcgaatac
     2521 cgacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg tacataccca
     2581 cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg atacgcagca
     2641 tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaatgca acgccagaac
     2701 cctgcgacag ccctttaccc tgcgaggtca tcgagtcagt agcgtactga acaacaaact
     2761 tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag ccgtctttcg
     2821 atgcgccatc aaccagacga tagttatcac gcaagttggc acgaccgtag tcgacaccca
     2881 gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg aaaacgtcgt
     2941 tcgcggtttc gttggtatag tcataaatat tgttgctggc gaaagaggaa gaaccaccag
     3001 cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg atgttttcca
     3061 gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga acgtcatgac
     3121 gttggtagaa gcgcttacct gcccagatgg tggagcctgg cagccattcg atcaggtttt
     3181 taccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca ttctgttgtg
     3241 cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct ttccacactt
     3301 cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg tatttacttt
     3361 gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccaa ccaataccgg
     3421 aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg cccgctgcga
     3481 cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct gagtcattgc
     3541 ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg cttaaacgcc
     3601 cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat ggcaacgctc
     3661 tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt cgttctggcg
     3721 gtacaccagg ttttgacgaa tggaagggat ctggatatgg atttgagttt cgttgccgag
     3781 ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac tcggcagtag
     3841 atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac ggctttcaac
     3901 tggcagccag acttgctgac gatttggcat cggcagctcc acctgcactt gatcgattgc
     3961 ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa atccggcgac
     4021 aaaacggtct gccggatagt ggtacagctc cagcggtttc ccaacctgcg ccacgcgacc
     4081 ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat cgtgggtgac
     4141 gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac gcatttgcac
     4201 acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc ttggctcggc
     4261 caccagcgta cggccaatcg ccacacgctg acgctgacca ccggagagcg ctttcggttt
     4321 gcgatccagc aaatgcgcca gttgtagcac ttccgccacc tggttaacgc gttggttaat
     4381 cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg ctactgacag
     4441 gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg ctggcggagt
     4501 gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct caagcccggc
     4561 aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca ccacgaattc
     4621 accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc aggctttcgt
     4681 tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc tgtcatcgac
     4741 agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat cctccacccc
     4801 ctggcttttt tatgggggag gaggcgggag gatgagaaca cggcttctgt gaactaaacc
     4861 gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt ttatgtgcgc
     4921 atctccacat taccgccaat tctgtaacag agatcacaca aagcgacggt ggggcgtagg
     4981 ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt aggtgttttc
     5041 acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg cacgcatcct
     5101 cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca aaatcgaaga
     5161 aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg
     5221 taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga
     5281 agagaaattc ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca
     5341 cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc
     5401 gttccaggac aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat
     5461 tgcttacccg atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa
     5521 cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa
     5581 gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga
     5641 cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga
     5701 taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat
     5761 gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat
     5821 gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt
     5881 aacggtactg ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc
     5941 aggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg aaaactatct
     6001 gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct
     6061 gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca tggaaaacgc
     6121 ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg
     6181 tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc
     6241 gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa cgccttgtcc
     6301 ggcctacaaa accgaaacgt atgtaggcct gataagacgc gtcagcgtcg catcaggcag
     6361 ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga accccatgga
     6421 tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag tgctaggtct
     6481 gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg aatacctgtt
     6541 cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca atcgtaaagc
     6601 ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg tcctcttccc
     6661 tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc agctgacttt
     6721 tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa cctataactt
     6781 tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg aaaccggcaa
     6841 aaattacctc tccgacgctt ttaaatttgg cggcgagcaa aaactgcaac tgaaagaaac
     6901 gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga atcgtcaggc
     6961 gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga gctccctgcg
     7021 ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt tgacgaataa
     7081 tcagagcggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt ccattaccgc
     7141 cgacggcaac tggggtgatg aaaagctaag ccccggttac accgtgacca ccggctggaa
     7201 aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg ccattttcgt
     7261 ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg gcatggttct
     7321 ggcgtgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg tcctgctgat
     7381 tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaagggt tgtttaacca
     7441 gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc ccgcctggtt
     7501 cagcgatccg accaccgccc gcacgatgct aattatcgtc aatacctggc tgggttatcc
     7561 gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt tgtatgaagc
     7621 ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc tgccgctgct
     7681 gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta acaacttcgt
     7741 gctgattcaa ctgttaacca acggcggccc ggatcgtctt ggcacgacca cgccagccgg
     7801 ttataccgac ctgcttgtta actacaccta ccgcatcgct tttgaaggcg gcgggggtca
     7861 ggacttcggt ctggcggcag caattgctac gctgatcttc ctgctggtgg gtgcgctggc
     7921 gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac aaaaatggca
     7981 atggtccaac cgaaatcgca aaaagctcgt ttatttatta ctcacctgct actgctactt
     8041 tttatcgcag cgattatgtt cccgctgctg atggtcgtcg ctatctcgct gcgtcaggga
     8101 aactttgcga ccggcagcct gatcccggag caaatctcct gggatcactg gaaactggcg
     8161 ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt cccggtactg
     8221 ctgtggctgt ggaactcggt aaaggtcgcc gggatttccg cgattggcat tgtggcgctc
     8281 tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc gacgctgctg
     8341 aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc gttgtatgcg
     8401 ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca cggcggcgta
     8461 attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg ctatttcgaa
     8521 accatcgaca gttcgctgga agaagctgct gcgctggatg gtgcgacacc gtggcaggcc
     8581 ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt catcctgtcg
     8641 tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga cgtaaacagc
     8701 tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct gtggggtgac
     8761 tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt gctggctcaa
     8821 cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg ttgttctgcc
     8881 aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactgt tattcggcgc
     8941 tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg ttaatatttc
     9001 catcatagag cttattattt ttacgttatt tgttttccca cttacgataa ttctctttcg
     9061 tgctctgagt cacggcaata gtattgtttt tatcaatttt ggataattat cacaattaag
     9121 atcacagaaa agacattacg taaacgcatt gtaaaaaatg ataattgcct taactgcctg
     9181 acaattccaa catcaatgca ctgataaaag atcagaatgg tctaaggcag gtctgaatga
     9241 atacccagta taattccagt tatatatttt cgattacctt agtcgctaca ttaggtggtt
     9301 tattatttgg ctacgacacc gccgttattt ccggtactgt tgagtcactc aataccgtct
     9361 ttgttgctcc acaaaactta agtgaatccg ctgccaactc cctgttaggg ttttgcgtgg
     9421 ccagcgctct gattggttgc atcatcggcg gtgccctcgg tggttattgc agtaaccgct
     9481 tcggtcgtcg tgattcactt aagattgctg ctgtcctgtt ttttatttct ggtgtaggtt
     9541 ctgcctggcc agaacttggt tttacctcta taaacccgga caacactgtg cctgtttatc
     9601 tggcaggtta tgtcccggaa tttgttattt atcgcattat tggcggtatt ggcgttggtt
     9661 tagcctcaat gctctcgcca atgtatattg cggaactggc tccagctcat attcgcggga
     9721 aactggtctc ttttaaccag tttgcgatta ttttcgggca acttttagtt tactgcgtaa
     9781 actattttat tgcccgttcc ggtgatgcca gctggctgaa tactgacggc tggcgttata
     9841 tgtttgcctc ggaatgtatc cctgcactgc tgttcttaat gctgctgtat accgtgccag
     9901 aaagtcctcg ctggctgatg tcgcgcggca agcaagaaca ggcggaaggt atcctgcgca
     9961 aaattatggg caacacgctt gcaactcagg cagtacagga aattaaacac tccctggatc
    10021 atggccgcaa aaccggtggt cgtctgctga tgtttggcgt gggcgtgatt gtaatcggcg
    10081 taatgctctc catcttccag caatttgtcg gcatcaatgt ggtgctgtac tacgcgccgg
    10141 aagtgttcaa aacgctgggg gccagcacgg atatcgcgct gttgcagacc attattgtcg
    10201 gagttatcaa cctcaccttc accgttctgg caattatgac ggtggataaa tttggtcgta
    10261 agccactgca aattatcggc gcactcggaa tggcaatcgg tatgtttagc ctcggtaccg
    10321 cgttttacac tcaggcaccg ggtattgtgg cgctactgtc gatgctgttc tatgttgccg
    10381 cctttgccat gtcctggggt ccggtatgct gggtactgct gtcggaaatc ttcccgaatg
    10441 ctattcgtgg taaagcgctg gcaatcgcgg tggcggccca gtggctggcg aactacttcg
    10501 tctcctggac cttcccgatg atggacaaaa actcctggct ggtggcccat ttccacaacg
    10561 gtttctccta ctggatttac ggttgtatgg gcgttctggc agcactgttt atgtggaaat
    10621 ttgtcccgga aaccaaaggt aaaacccttg aggagctgga agcgctctgg gaaccggaaa
    10681 cgaagaaaac acaacaaact gctacgctgt aatcttcctg tccagcacgc cgcgccattt
    10741 cggcgtgctg actttttact cccgcttcag ccgtttcgaa ttacacagcc acagggtgat
    10801 caccagtaac aggatcgcag ccgagtagat caacacatcc agtggcgatt tatgatcgac
    10861 gatgatcaag cgcacaatgg cggtgatccc aatatagaca aagtaacgta gcgggaagtg
    10921 aaaaccggac tgaaagtact tcacaatcag cgcgataaat tcgaaataga gaaagtaaac
    10981 caccagtcct tctaccagct catatttgct ggtttgttct ggcgcgaaca gcacatcagc
    11041 cagatgcacc gtttctttgc cgaggaagac aaccaaaatc aggccgaggc acagcaggcc
    11101 aagattgagt acggtctgca aaatggtgga gataaactcc acgcgcggac gagagagtga
    11161 cgtcatagca gtacctcctt cacagtggcg agcttcctgt ataacgcaaa aatgtgacgg
    11221 agatctatat tttttgtgta tgttttgttc aacctggatt tggagcgcgc ctccaccaaa
    11281 gccggatata cctacccgta ggccggataa gccgcgcctg gcggcgcatc cggcaagaca
    11341 aaccgcacca aagccggtaa ttcaaaaatc acccattcag gtgattccca gccaccagca
    11401 atgcgcgaaa aatcgacaat gttcctctgg aacaaatgaa acgtaacctg ataaaggtgg
    11461 taaaaatgaa gccttatttt gctgctttga tgttatcagt ctctgttctt cctgcttatg
    11521 ctggtccgct cggtactgct gataaagccg acctaccgca aagcaatgtc tccagcccaa
    11581 tgatggccca gtcgctcagg caaccagacc tgcaaccgat atcgacggac aggaagacag
    11641 aatgttttcg cctttacaca ccagatcgca aaccgggagt gaactgcgtt cctgatggct
    11701 cgacaggcca ttaagaatga aggtgccagt gagtgcgaaa actggcacct ttcccatgac
    11761 ttagcggaaa ttgacgctac ggtcgctggt catgtcatac aactggaact tacgcccaag
    11821 ttgctgacca ccgtcacgcg tcagcggcgt ccagccaatc gccgcacggc tgcgtgtcgg
    11881 gccagacgag aagagatcca acggtaccga gacatacacg cctttggtga agtccccttc
    11941 gccgtactcc tctttcgaaa cattagtgat cgtggcatag ccacccacca ccacgccgct
    12001 atcaaagcgt ttggcgatct ccagcgtgcc gcctttatcc cctgccagat actgcccgac
    12061 gctggcttta actaacacat cctgagcgaa agatggcgtc cagtaggcgg tcagatgtcc
    12121 ggttttcacg ctgtagtcgg tgaatttcat catatctttt gcactacgcc agtcgcgctg
    12181 tttaacgtag ttggcatcca gaccaaacgc ccagttgctg tccagcgggc gatacaacac
    12241 ttctgccccc gcaccgccaa acatggtttc gagataacca ccgtagacct gaccgtagaa
    12301 gccgttgccc agatgctgga agtagttggc ttgcaggtta ttcacataga catcgttctg
    12361 cacatactcg cgcacatggg tacgcacgcg cggtaagtgc gagtcctgcg gaggattagt
    12421 gtagttaaac ttgtcgtagt tgttggcaag atttgcaaac aggctgccgg tggtcagcag
    12481 atggtccgtc agccacaaat ctgccgttcc catcacgccc agctgataca tgtaaaagtt
    12541 ttccgggcca ccgaccgact ggttcagcac cggatcgata tggaaatcaa agcgtgattt
    12601 gtcgatatac cagccctgct cggtggactg cggaaccact ggctcgacgc gtttttgcgc
    12661 cagcgtcgtt tcgtggccca acggctctcc ggcgagatga tttttcaggc tggcgacatc
    12721 ggtttccgtc gtcacctgcg gcatgttaag gcgattttcc gtaatgcgga tcgttttgat
    12781 cccatccggc agatcgttca tcacgatccg attcgcacgg atgatccctt cgcgcgaatc
    12841 acgatatttc acctgctcgc cggtaacata cagcgtatcg cctttcgcct ggatctgtgg
    12901 atcggcaagt ccggcattgt atttcaacag cgttaactga ttcgccacca ccgaatactg
    12961 caaaatggca tcctgcggct gcggttgata ttgcgggcgg gcgttatcgt tgtaagacgg
    13021 gcgcagatcg ttaaagttgg tgcgcaacgt aacgccaaac ataaaggtgt tgccacgttc
    13081 atagctaagg ttaacgtcgg cccaatcggt aacgcgataa atcgcaccga cgttaaactt
    13141 acttttttgc tccagcttcc cggcaaaatc ctgctgataa ttattgcctt catactccag
    13201 tttcaggcgc agcggttgcc agggcgtctg gtattccacg ccgccaaaca gtgaggcagg
    13261 accgtggaac atctggctac cgtcgataga tcccgcctgt ttgtagctgt tatcgcgata
    13321 gcaatattta tcactggctg aacagagcgg atttttcaca ttaccgctgg tgcccaaata
    13381 tccccagccc aggccgagcg taaaatcgaa cggcccccag gctttgctgg caacaagata
    13441 ttccgcatca aacagccccg taccgccgat atcccgcgca ccaaccgcca cttgcggcag
    13501 ccagtaactc tcttcccaca aacgcagttt gagatcgaag gctttatctt tatacgtttg
    13561 atcgccagag aacgcttcga cgctgctgta ctgccgggtg cgcacgtcgg tgtagcgcag
    13621 cgttgtttcc agccacggga agagttgcac tgaagctgag taataacggt actgatcgtt
    13681 atcgcgatag ttcagactca actccccttc ccgtgccata cgcgcggtgg gcgtttgtaa
    13741 taatcctacg ccaccgaaat ccgactgcga cggaccaatg ggcgcaggat atgtttcgcc
    13801 gtagcaagct gtgctaatgc ccagcgccag taagctaagc agatgtcttt ttttcattat
    13861 tgaggtatcc gctgcgtcag agtctgaaga atgtcggcgt taagggcatc aggcgtctca
    13921 ctccagacgg agtccgcgag gccaacataa ataatgctgc cgggcatcgg ctctacgtga
    13981 cgcttgttcc agtaagccac cggcgctttt tgcgtgcgtc cgtccgggta aaccacccac
    14041 gcgtagctgc gatccgcacc gctgagcagg ctctggtcag agagatagct cgccacgtcg
    14101 cgacctggag tgaatggctg cttgccagga cggctgataa gcccgaacaa cgtgaccgtg
    14161 gacggcggtg gtccgaccca cagcgtgtag ttgccctgca acggcgggtt accgcgttcg
    14221 gcaacgcgga cgatatcggg atcaagattg attttttgcc tgcccgtcac tttcaacgcc
    14281 tgaatttgct ggcgtaaggc gttaatcgca gcggcatcgt cggcgctgga atctgcgccc
    14341 tgttctgcca gccgtgtcag caacgcctgc tgctgacgca atgctgccgc cgttgccagc
    14401 tcttcgctaa tcaccgcacc gggccaccag ctgtttgcca gccgcggttg gcctaccaga
    14461 tcgattaaat gctctgcgcc cgttagcgtt ttggcctcac tgctgccatt gctgaacacc
    14521 ttgacggttc ctgccgcgaa gaccgatgac gctcccacac tcaaaagcaa cgcgacaata
    14581 gtttgtttaa tcattgtttt gccgccttga tgagagtggt tttcaccgga aaataatccg
    14641 cgccgagata ctgttccgac tggcgaattt gcccttcgct gtcgatccaa tagcggttat
    14701 gccagcgcgc ctggtcggtg gagacttctt cgtccagcac gcgaaccggg gtttcatcgc
    14761 tgccgacttt gacggtatcg gtgccatccc atttgaagac tgagcgtgcg gtggcgtagc
    14821 gtacctgctg gtactcggtc cagcccatcg tgcgcgtcca gcttgcgcca tcaacgattt
    14881 gtgcgggttt aatcagcggg tcggcagcaa ggttattcac ttcaatcagg ttgtcgccgc
    14941 cgagcagagt cttcaccaga cggccatgtt gggtaacgag agtagcctga tcctgagtga
    15001 cccatttttg ttgtccgtct tcagcgaagg ccagcaccac aaataactgc ggcccgccat
    15061 taagctgcat gtactggctg gcgtagggca tattttgaat atcatcatcc gtcagctgta
    15121 cgcctggcgt gccgaacaga ctgtcccaca gtgaattgcc cagctcttta gtcgtggctg
    15181 aacaggcctg taataacagg cagataagaa tgagtgcagg tcgcttcacg actcttctcc
    15241 agaggggcag aataaccaca ccggagtgtg gttatggtta atcccttcat acttctagcc
    15301 acagatgcaa tgactcgaat tattttgggg atacatactt tattactggg tactggtggt
    15361 cgtggttgtg gtggtcccgg tattagaacc atcaccgcca ccggttgccg caagcgcgac
    15421 acccaccgcc gagcttacgg tgctgacgct ggtcgcggag gaactccccg ccgaaaccga
    15481 cgttgctgcc gaccctgccg cttcgcccac ctgtacaggt gcagcccacg cagaagtcgc
    15541 cgcaagcgca gatatggcaa aaatgccata cagaactttt ttcataacaa tttcccttca
    15601 ttgaatgaat ggagatttac ccaaaaacat ttcgggcggg atcgagtata cacaactaaa
    15661 gcatgcgaat tacggcgcgg ggaattagcg atggcgtttt aggataattg gaatgaatat
    15721 caaatataag tttcaactta tatttaaata acaataaaaa tcataaactt aaaataaaaa
    15781 caaaagcaat taatctgcgt attttccgaa ataaacaacg attaaaaatc tattttagga
    15841 ttaacctcat ggtatgattt ccgttaaatc acagacaagg ggatttatct gataaaaaaa
    15901 tgccgacata acgtcggcat tgttattaag gttatttaat caatcgtgct atgtattaat
    15961 tgccgaatac gatgtacata tcggcatcga cctgtaggcc tgataagacg cgaccgcgtc
    16021 gcatcaggca tcggttgccg gatgcggcgt gaacgcctta tccggcctac atatcgacga
    16081 tgattaaccg cgccacgctt tatagcggtt aatcagacca ttggtcgagc tatcgtggct
    16141 gctgatttct ttatcatctt tcagctctgg cagaatacgg ttcgccagct gtttacccag
    16201 ttccacgccc cactggtcga aggtgaagat gttcaggatc acgccctgag taaagatttt
    16261 gtgctcatac agcgcaatca acgcacccag gctgaacgga gtgatttcac gcagcaggat
    16321 ggagttggtc gggcggttac cttcgaatac tttgaacggc accacgtagt caagcgttgc
    16381 cggatcttta ccctgatcac gatattcctg ctcaaccact tcgcgggatt taccaaacgc
    16441 cagcgcttcg gtctgggcga agaagttaga cagcagtttc tggtgatgat cagagagcgg
    16501 gttatgggtg atagccggag cgatgaaatc gcacggtacc attttggttc cctggtggat
    16561 cagctggtag aacgcgtgct gaccgttagt gcctggttca ccccagataa tcgggccagt
    16621 ctggtaatcc acaacgttac cgttacggtc aacatactta ccgttggact ccatattgcc
    16681 ctgctggaag tacgccgcga aacggtgcat atactggtca tacggcagaa tcgcttcagt
    16741 ttccgcacca aagaaattgt tgtaccagat gccaatcagc gccagcagta caggcaggtt
    16801 tttctcggca ggcgtggtgg agaaatgctt gtccatcgcg tgtgcgccgg aaagcagttc
    16861 aacgaagtta tcaaagccga tggagagaac aatcgacagg ccaatcgctg accacaaaga
    16921 gtaacggccg ccaacccagt cccagaactc gaacatgttg gcagtatcaa taccaaactc
    16981 gccaacggct ttggcattgg tggaaagcgc cgcaaagtgt tttgcaacgt gtttttcatc
    17041 acctgccgct ttcaggaacc agtcacgcgc gctatgggcg ttggtcatag tttcctgagt
    17101 ggtgaaggtt ttagatgcta ccaggaacag cgtggtttcc gggtttactt ttttcagcac
    17161 ttccgcgatg tgagtcccat cgacgttaga aacaaagtgc atgttcaggt ggtttttgta
    17221 cggacgcaga gcttcggtca ccatgtatgg gccgaggtca gaaccgccga tcccgatgtt
    17281 cactacgtca gtgattgctt tgccggtata acctttccac tcaccggaaa taatcgcttc
    17341 tgagaaggtt ttcatcttct ccagcaccgc gttgacttcc ggcattacgt ctttgccatc
    17401 aaccaaaatc ggggtattgc tacggttacg cagcgctacg tgcagcacgg cgcggttttc
    17461 agtgcggttg atcttctcgc cagagaacat cgacttaatc gcgcccgcca gatcgcactc
    17521 tttcgccaga tcctgtaatt tcgccagcgt ctcttcagtg atgcggtttt tggagtaatc
    17581 caccagcatc tgatcgtcga aggttgcgga gaacttagaa aaacgatcgc cgtctttagc
    17641 aaaaagatcg gcgatcgtaa cgtctttcat ttcatcgaag tgtttctgta gtgcctgcca
    17701 ggcagcggtc tgcgttggat tgatgttttt cattagcaat actcttctga ttttgagaat
    17761 tgtgactttg gaagattgta gcgccagtca cagaaaaatg tgatggtttt agtgccgtta
    17821 gcgtaatgtt gagtgtaaac ccttagcgca gtgaagcatt tattagctga actactgacc
    17881 gccaggagtg gatgaaaaat ccgcatgacc ccatcgttga caaccgcccc gctcaccctt
    17941 tatttataaa tgtactacct gcgctagcgc aggccagaag aggcgcgttg cccaagtaac
    18001 ggtgttggag gagccagtcc tgtgataaca cctgaggggg tgcatcgccg aggtgattga
    18061 acggctggcc acgttcatca tcggctacag gggctgaatc ccctgggttg tcaccagaag
    18121 cgttcgcagt cgggcgtttc gcaagtggtg gagcacttct gggtgaaaat agtagcgaag
    18181 tatcgctctg cgcccacccg tcttccgctc ttcccttgtg ccaaggctga aaatggatcc
    18241 cctgacacga ggtagttatg tctgaaattg ttgtctccaa atttggcggt accagcgtag
    18301 ctgattttga cgccatgaac cgcagcgctg atattgtgct ttctgatgcc aacgtgcgtt
    18361 tagttgtcct ctcggcttct gctggtatca ctaatctgct ggtcgcttta gctgaaggac
    18421 tggaacctgg cgagcgattc gaaaaactcg acgctatccg caacatccag tttgccattc
    18481 tggaacgtct gcgttacccg aacgttatcc gtgaagagat tgaacgtctg ctggagaaca
    18541 ttactgttct ggcagaagcg gcggcgctgg caacgtctcc ggcgctgaca gatgagctgg
    18601 tcagccacgg cgagctgatg tcgaccctgc tgtttgttga gatcctgcgc gaacgcgatg
    18661 ttcaggcaca gtggtttgat gtacgtaaag tgatgcgtac caacgaccga tttggtcgtg
    18721 cagagccaga tatagccgcg ctggcggaac tggccgcgct gcagctgctc ccacgtctca
    18781 atgaaggctt agtgatcacc cagggattta tcggtagcga aaataaaggt cgtacaacga
    18841 cgcttggccg tggaggcagc gattatacgg cagccttgct ggcggaggct ttacacgcat
    18901 ctcgtgttga tatctggacc gacgtcccgg gcatctacac caccgatcca cgcgtagttt
    18961 ccgcagcaaa acgcattgat gaaatcgcgt ttgccgaagc ggcagagatg gcaacttttg
    19021 gtgcaaaagt actgcatccg gcaacgttgc tacccgcagt acgcagcgat atcccggtct
    19081 ttgtcggctc cagcaaagac ccacgcgcag gtggtacgct ggtgtgcaat aaaactgaaa
    19141 atccgccgct gttccgcgct ctggcgcttc gtcgcaatca gactctgctc actttgcaca
    19201 gcctgaatat gctgcattct cgcggtttcc tcgcggaagt tttcggcatc ctcgcgcggc
    19261 ataatatttc ggtagactta atcaccacgt cagaagtgag cgtggcatta acccttgata
    19321 ccaccggttc aacctccact ggcgatacgt tgctgacgca atctctgctg atggagcttt
    19381 ccgcactgtg tcgggtggag gtggaagaag gtctggcgct ggtcgcgttg attggcaatg
    19441 acctgtcaaa agcctgcggc gttggcaaag aggtattcgg cgtactggaa ccgttcaaca
    19501 ttcgcatgat ttgttatggc gcatccagcc ataacctgtg cttcctggtg cccggcgaag
    19561 atgccgagca ggtggtgcaa aaactgcata gtaatttgtt tgagtaaata ctgtatggcc
    19621 tggaagctat atttcgggcc gtattgattt tcttgtcact atgctcatca ataaacgagc
    19681 ctgtactctg ttaaccagcg tctttatcgg agaataattg cctttaattt ttttatctgc
    19741 atctctaatt aattatcgaa agagataaat agttaagaga aggcaaaatg aatattatca
    19801 gttctgctcg caaacgaatt cccgtacact gaactctctg gctcactgag ggcaccatca
    19861 tggcactccc ccgtattacc caaaaagaga tgactgaacg cgagcagcgt gaacttaaaa
    19921 cactgctgga tcgggcgcgg attgcgcatg gtcgcgtatt gaccaactcc gaaaccaaca
    19981 gcattaagaa agattacatc gataaattaa tggttgagcg tgaggctgaa gcgaaaaaag
    20041 cccgccagtt gaagaaaaag caggcttata aaccggatcc tgaagcgtca tttagctggt
    20101 cagcaaatac gtcgacgcgc ggcaggcgct aatccacaat caacgcctga gattttcact
    20161 cgtcagtgtg aacacaattt tcccctcacc ctaacccttt tcccgaaggg gcgaggggac
    20221 tgtccgggca cttttgggcc gttttcatca gtctgaggcg ttaatcagcg ccccttcttt
    20281 ttacgccccg gcgaggtaaa acgcttaccg ttggacgccg ggcgaccgcc tttttccgcc
    20341 gttttttcca tcttaacgac tggacgtttg atgcccgctg ttttcggttt ggccttcgct
    20401 ttaggtttta cctcggaaga ggaattttca atgagcttaa agaggtcgat taactcatcg
    20461 tcggttaaat cgcgccattc ccccagcgga atgccgctta agctaacgtt catgatgcgc
    20521 gtgcgttcca gctttttcac ttcatagccg aaatgctcgc acatgcgccg gatctgacgg
    20581 ttcagcccct gcaccagggt aatgcggaag acaaacggcg cttctttttt aactttgcac
    20641 tttttggtca ctgtcccgag gattggcacc cccgcactca tgccgcgaat aaactcctcg
    20701 gtaatcggtt tatcgaccgt caccagatac tctttctcat gatcattgcc agcacgcagg
    20761 atcttattca ccagatcgcc gtgattggtg aggaaaatca gcccctggga gtctttatcc
    20821 aggcggccaa tcgggaacac gcgtttgctg tggttaacga aatcgacaat gttatcgcgc
    20881 tcgccatctt cggtggtgct tacaatacca acgggcttgt tcagggcgat aagtaccaaa
    20941 tcttcggctt cccgaggttc aatcaactga ccgtttactt tcacaacgtc gccgggtttc
    21001 acctgatcgc caatggtggc tcgcttgcca ttaaggaaca cattgccttg ctcgatatag
    21061 cgatccgctt cgcggcgtga gcaaattccg ctttcgctga tgtatttatt taaacggact
    21121 gatgagtcgg gcagcataaa ttctcctgta aaaaacggaa tataccgtac ctttgggttg
    21181 ataaaaaata gtcatgagcg aactatttgt tatttccatt ggcttgaatt cgttacctgc
    21241 tcaaatcact ttccagaagc aatgtgcgac atcgtgagcc atgtcagtaa gtggttaaat
    21301 tcgcagtggt agcatgcctc ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
    21361 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
    21421 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
    21481 acctgggatg attacacagc gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
    21541 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgctga gatcgtgatt
    21601 gtcggcggcg ggaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggca
    21661 ccaattactg acgtggttaa acgtggcgct ctgtatattg gctggagcgc aggcgctaac
    21721 cttgcttgcc caactattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
    21781 gatgcgctaa atctgttccc gctgcaaatc aacccgcact tcaccaacgc gctgccggaa
    21841 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
    21901 ctgacgatta ttggtctacc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
    21961 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
    22021 gctggtcacc gtttttaagt gaattgccgg atgcatgatc cggcatcttg ttcatagtgc
    22081 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
    22141 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
    22201 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
    22261 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
    22321 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
    22381 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
    22441 cccatcaagc gaaaatgccc gccgtgctgc cagtgatttg tcagcaattt cgctgcccat
    22501 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctctgccca
    22561 gcggcgcgac tcttcctccg ccagctcttc ttttggcatc cgcgccagat acagcttaat
    22621 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
    22681 ctcaccgtgc atcactttat tcaacccttc catcatctgt tccatggcgt cgccaatgcg
    22741 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtatccagcg cgctgacatc
    22801 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
    22861 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
    22921 gacgtggaaa tagatcacca gttccgcttt tggcaatgac aacttcccca ttgtctctgc
    22981 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
    23041 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcattg ccagcagacc
    23101 ggaaccgagg ttagcaccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
    23161 ggcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
    23221 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
    23281 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgctagcaa
    23341 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
    23401 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
    23461 gagaatacgc gccattagcg ccgtcccgac atctgcaccc agcacaatga ccagagccgg
    23521 tgcgagggct accagatcct gagcgacaaa cgaggtcacc agcatggtgg tggcattact
    23581 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
    23641 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
    23701 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
    23761 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
    23821 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
    23881 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
    23941 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
    24001 attcggtgcc agccagcgct caacttcggt aacgctcata cctttacggc gggcataatc
    24061 ttcaacctga tcgcgctgaa tttgtgctac agcgtagtac ttgctgtccg ggtggctgaa
    24121 gtaccaaccc gaaaccgatg caccgggcca catggcgaaa gattctgtga gtttcatgcc
    24181 agtgtgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
    24241 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gctcttcgtt
    24301 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
    24361 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
    24421 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
    24481 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
    24541 gtagttagcg aagcctgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
    24601 atgggtacgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
    24661 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
    24721 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
    24781 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
    24841 cagcgtttcg atgctggctt cgacttcctg cacgccgaga cggtgcgcca ccggcggcgt
    24901 gtaagcctgc cagtcaaaag cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
    24961 tgtgcgcggt ttcttgcgcc cgtgctgaat acgtacggtt tcgtactcct tgcgggtacg
    25021 agcgacaaaa tcatcacgct gggtatcgga aagcagcgcc gccaccacac caacggtacg
    25081 cgaggcattc tgcacataca ccgtcgggcc gctgtagttc tgctcgattt tcaccgccgt
    25141 gtgcgctttt gaggtcgtcg cgccgccaat cagtaacgga atagtgaagc cctgacgctc
    25201 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
    25261 aatcagatca gcattcactt ctttagcggt acggagaatt ttttccgcag gcaccataac
    25321 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
    25381 gatgtcgtgg acgtcgccct tcacggtggc gatcaccatc ttgccgttgg ttttgccctg
    25441 ctctttgctg gcttcaataa acggttcgag gtaggccacc gcctgtttca tgacgcgcgc
    25501 cgatttgacc acctgtggca ggaacatttt cccttcgcca aacaggtcgc cgaccacatt
    25561 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
    25621 ttcttcggta tcctgctcga taaactcggt aatgcctttg accagcgagt attccagacg
    25681 tttattcact tcccacgagc gccactccgc ctgctgggcg ttggcggtgt cgtcggtttt
    25741 gctgccgcga tatttctcgg caagctccag taaacgctcg gtgccatcgt cgcgacgatt
    25801 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
    25861 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
    25921 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaac gagaaagaaa cgttagatac
    25981 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
    26041 gtcctgcgcg tagttgttgt gctcttcaat gccagttgcg accgcgaaga tgtttgggtc
    26101 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    26161 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccattac
    26221 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg catcgacgcc
    26281 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    26341 aatgacgtcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    26401 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    26461 gttgatatcg ataatctgcg cgccgttttc cacctgttga cgcgcgacat ccagcgcctc
    26521 gctgtatttc tcttctttga tcaggcgctt gaacttagcg gaaccggtga cgttggtgcg
    26581 ttcacccacg ttcacaaaca ggctatcttc gccaatgttc agcggctcca ggccggacaa
    26641 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    26701 catcgctgca atatgttgtg gcgtggtgcc acagcagccg ccgacgatat tgagaaaacc
    26761 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtactcacc
    26821 aaaggcgttg ggtagcccgg cgttcgggtg cgcggtgacg tagcattccg caatccgtga
    26881 cagctcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    26941 cagagcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    27001 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    27061 ttcaaactcc gttttcaccg caaataccgc cgctttggcg ttaagggtgt cgaaaacggt
    27121 ttcaatcagg atcagatccg cgccaccttc caccagcgct ttggtggact ctcgataagc
    27181 cgccaccagc ccgtcaaaag tgatattacg aaatgccgga tcgttgacgt ccggagaaat
    27241 agacgccgtg cggttggtcg ggccgagaac accggcaacg tagcgcggtt tctctggcgt
    27301 gcgcgcggtc cactcgtcag cacaagctcg cgccagtttc gccgccgcaa agttgatttc
    27361 cgccgacagg gattccatct ggtaatccgc catcgcaatg gtcgtggagt tgaaggtgtt
    27421 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    27481 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    27541 gcgttcacca cgaaaatcgg cttcgttcag tcgataactc tggatcatgg tgcccatacc
    27601 gccgtccagc accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    27661 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    27721 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    27781 catcggatca gttcagtaac tattgcatta gctaacaata aaaatgaaaa tgatttccac
    27841 gatacagaaa aaggagactg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    27901 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    27961 actggagtgg attgccgaat ccaatggcag tgtggcactc acggaactgg cgcaacaagc
    28021 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    28081 gcgtcaggtt ggcgaactgg gacattgggc aatcggcgca catgccttta tggtcggcag
    28141 cagctttctc cagagccgta atttgttagc gattgttcac cctatcctgc gcaatctaat
    28201 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    28261 tattatcgac caggtacagt gtacgcatct gatgcgaatg tccgcgccta tcggcggtaa
    28321 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    28381 ggtgacgaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa cgctggtgtc
    28441 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    28501 tgaggaacat gcactggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgtga
    28561 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg accgcgtgac
    28621 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    28681 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    28741 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    28801 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    28861 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    28921 tttctttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    28981 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    29041 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    29101 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    29161 agaactgtag acaaaatggt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    29221 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    29281 cgacacttac tcaaaccatt gccccaatat ttgaaaaata cctaatggaa attttacctc
    29341 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    29401 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgaccc caaaaccgta
    29461 tagtatgccg acacattgct gcacaatata ttaatgatat ttatcagaat gttgattaca
    29521 aaccccatca agatgattac tcttcagctg aaaaatttct cacgcacttc aacaaaaaat
    29581 gcaaaaacca gactttggcg ttggtttcca gccgtcctga ggggcgttgc gttgctgcct
    29641 gcggtgattt cgggctagtt atgaaagcat attttgacaa gatggaatca aatggcatca
    29701 gtgttatggc agccatatta ctggtggata accatgcttt gacggtccgg ctaagaataa
    29761 agaacacaac tgaaggatgt acccattacg tggtttcggt ttatgatcct aatgtaacta
    29821 acgataaaat aagaattatg agcgaaagca aagagaatat taaacactat tctctgatgg
    29881 attttatgaa tgtagattat agcctcctga aatggtcaaa tgatcatgtt attaatcaat
    29941 ctgttgcaat aattccagca cttccgaaag aacagctatt gatgttaaaa ggatctgtgg
    30001 atgaaataac ccctccatta tcacctgcaa cgatgaattt gctaatggca attggtcaga
    30061 atcaccaact tacgcaactg atgattcaac tccagaaaat gccagaacta catagaacag
    30121 aaatgttgac tgcctataat agtattaacc tgccaggttt atatttggct ataaattatg
    30181 gtaatgcgga tatcgttgag actattttca attcattgtc agaaacagga tatgaaggat
    30241 tactctcgaa aaaaaatctc atgcatattc tggaggcaaa agataaaaat ggtttttctg
    30301 gattattttt agcgatatca cgtaaggata aaaatgttgt aacctcgatt ctgaacgcct
    30361 tacctaaact ggccgcaaca catcatttag ataacgaaca agtgtataaa ttcctgagtg
    30421 ccaaaaatag aacgtccagc catgttttat accatgttat ggcgaatggt gatgccgaca
    30481 tgctgaaaat tgttttgaac gcgttacctt tgttaattcg cacatgtcat ttgactaaag
    30541 aacaggtact cgatctcctg aaggcaaagg atttttatgg ttgcccaaga ctatatctgg
    30601 cgatgcaaaa tggacatagc gatatcgtga aagttattct tgaagcattg cccagcctag
    30661 cccaggaaat taacatttca gcttccgata ttgtagatct tctgaccgct aaaagtcttg
    30721 cgcgcgacac gggtttgttt atggccatgc agcgcggaca tatgaacgtt attaatacta
    30781 tttttaacgc attacccact ctgtttaata cgtttaaatt cgataaaaaa aatatgaagc
    30841 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
    30901 aacaacaaaa tgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
    30961 gatttaccgc tgaagatatt atggattttt ggcaacacaa agccccacaa aaatactctg
    31021 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
    31081 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
    31141 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
    31201 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
    31261 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
    31321 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
    31381 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
    31441 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
    31501 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
    31561 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
    31621 ccagatattg agcggcacca tccgccgctc aatataaaga tggcgaatca caatttgttc
    31681 gccgagatcg gtgatttttt ccgctgcttc ctgaagcagt aattccatta atgccgggga
    31741 aatatgccgc ttctccagca caaagttttc aaactcctgg gtgtccgcca ttcggcccac
    31801 gcgatcgtgc tctttcacca gttgatagca ggcacgaacg tgagcggcag acatctcttt
    31861 ctgcggcgcg aacctgtctt tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa
    31921 caccaacatc accattccac gaatacccgg cgcttcaatg aactgctcat tacagccctg
    31981 tagataaacg agatattcgc ggtagctttc ggttttggcg tgcttctggc agccgatagc
    32041 catatacaat tcagcggtgg ttttacctgg cagaatttcc cgtagccact cgaccagcgc
    32101 tgcgggcagc ggcgcataaa ccataaaata agaacgcgca aagccaaaaa caatgctcgc
    32161 ttcggcggtc gtcgtcaggc aggtatcaat aaataactcg ccgtcgtccg tctggtggat
    32221 cggcagcaaa aatggcaatg tgccggaagg tgtgatcagt ttgcctacca gccaggcggc
    32281 tttattgcgg taaaacagtt cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt
    32341 ccccagcgtt tccgtcagat ggcgaataat gtaatggatg tcacggcttt tattctgcca
    32401 gcgcaggcgc agcggtaggt cgctgataac gcgcatcagt agagattccc agccgtgatc
    32461 ggggtgaaag tctttcgcca gcgggcgggg aatggtacga aagcggcgct ctggctgaga
    32521 gctaaagata aaaagccgct cgggagtaag cgagcggtgg tcaaataacc gacagtacac
    32581 ggagttaaaa aagctctccg caatctcgaa gcgcgggtaa tccggcaaca gccgggtgta
    32641 atgctcttta acacgtagta aaaatgccgc gtccgtactt tggccgttag taatgcagcg
    32701 cagttgctcc acgaccagac caacgtgatg atcgtaaaga tggatacggt ttttcatcgc
    32761 ctgctggaca gcatgccagt cggcctgttc gaaacgctgc tgcgcaccgg aggtcacttc
    32821 gaggaatcga ccatactgag catcgaagcc ttgcaaaatg gtttgagcaa tcaataattc
    32881 caggccacgc ggcatttagc gccctcatca ggagcagaga attgccggat gcggcgtaaa
    32941 cgccttatcc agcctacgtt cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc
    33001 atcaggcatc gtgcaccgat tgccggatgc ggcgtaaatg ccttatccgg cctacgttcg
    33061 gcaacggctg taggcatgat aagacgcgca agcgtcgcat caggcatcgt gcaccgattg
    33121 ccggatgcgg cgtgaacgcc ttatccggcc tacagtcagc aacggttgtt gttgcttaga
    33181 actgcgattc ttcagtggag ccggtcagcg cggtgactga agacgtgccg ccctgaataa
    33241 tagtcgtcac tttatcgaag taacctgtac ccacttcctg ctggtgagat acgaaggtat
    33301 agccatcttt cgcggcggca aattccggct gctgcacttt ctcaacgtag tgcttcatac
    33361 cctcgccctg ggcataggcg tttgccaggt caaacatgtt gaaccacatg ctgtggatac
    33421 ctgccagggt gatgaactgg aacttgtagc ccatatccga cagctgctgc tggaagctgg
    33481 caatagtttt gtcgtcgagg tttttctgcc agttgaacga cggcgagcag ttataagcca
    33541 gcagtttgcc cggatatttc gcgtggatag cttgtgcaaa gcgacgcgcc agttccagat
    33601 ccggcgtgga ggtttcacac cagaccaggt cagcatatgg cgcatacgcc aggccacggc
    33661 tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc ttcactggta cgctcgccgg
    33721 taataaattc gctgtcatac gggtcgcaat cggaggtgat cagatccgcc gcatcagcat
    33781 cggtacgggc aaccagcagg gttggaacgc ccgtcacgtc agctgccaga cgcgccgcga
    33841 ccagtttctg aatagcttcc tgagttggca ctaaaacttt gccgcccatg tgaccgcatt
    33901 tcttcactga cgccagctga tcttcgaagt gaactgccgc tgcaccggct tcaatcatcg
    33961 ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc ggcttccgca tcggcaacga
    34021 tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc aatgcccgcg gaccattgga
    34081 tctgatcggc acgacggaag gtgttgttga tccgctccac cacagctggc accgagtttg
    34141 ccggatagag cgactgatcc ggatacatgc tggccgccag gttagcgtcc gccgctacct
    34201 gccatcccga cagatagact gcttcaatac ccgctttcgc ctgttgcagc gcctgaccgc
    34261 cagtcagtgc gccgaggctg ttgatgtagc cttttttcga ctcaccgtgc agcagacgcc
    34321 acattttcgc tgcgcccagt tgcgccagcg tgcattcagg attgactgaa ccgcgtaatt
    34381 tcaccacatc ttccgcactg tatgggcgag taatgccttc ccaacgcggt tgagtccact
    34441 ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc tccatagtta
    34501 tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc aggaaatcaa ttaactcatc
    34561 ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca aaacgcccct gggagaaacg
    34621 ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct ggcggaacaa
    34681 ggctttggtc accggtttgc cattgctcaa cgttttttga tgatggatcc actgccagat
    34741 cgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa tcggcacaca
    34801 gccgttgcca gagatccacg cttcgatgta ctgcacagcc acgcgaatgt tggcgcgcat
    34861 accttcttcg gtgcgttcac catcacaagg tgccagcagc tgatcggcag taatcggcgc
    34921 gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt cgttgaatac
    34981 cgccattgcc gtgtccgcaa ggcctgggtg agcgatccat gtgccatcgt gaccgttatt
    35041 ggcttccagc gatttatccg cttttacttt gttgagcacc tggttattgt gctcttcatc
    35101 tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt tatggcaggt
    35161 tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtca ctgcctgtct
    35221 gtctggcagg acgcgatcgg gatagttttt caacgttttg atatagctga agatgtaatc
    35281 ccaacgaccg cagttcagac caacaatatg gtcacgcagc gcgtgaagga tttcatccat
    35341 ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag
    35401 attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt cctgccagga
    35461 ctgggttttc ggcagataga aatagggacc actgcccttt gccaacagtg cctgatagtt
    35521 gtggaagaaa tagagcgcaa aatcaaacag gctgccgggg attgcctcac cacgccaggt
    35581 gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg ctggattggg
    35641 cttgagctgg taaattttgc ctgcttcatt ggtgtaactg atggtgccgt taaccgcatc
    35701 acgcaggtta atttgcccgt cgatcacttt gttccagtct ggtgccagtg aatcttcgaa
    35761 atcggccata aagactttca cattggcgtt gagcgcgttg atcaccatct tgcgctctac
    35821 cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc gaattttcca
    35881 atcagcatag cgaatggaag ctgtttccga aataaaatca ggcaacgttc cgttatcaat
    35941 atcttgctgc tgctgaatgc gcgctgccag aagtttattg cgttgtggcg taaaatgcgt
    36001 caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct cctgctcgcc
    36061 atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca tcgtgcagct
    36121 cctcgtcatg gatccgaaaa cttccccact gtgaacgaag gatcgttgtg ctcttttcgt
    36181 tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc cattttaatt
    36241 aaaaatactt gataacttgt tgataacaat atgaataaaa tttattcacc tgctgaggta
    36301 cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga atcgcttaac
    36361 gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga tcgtatggcg
    36421 tgatctggta gacgtaatag ttgagccagt tggtaaacag taaattaccg tgactacgcc
    36481 agctcgctcg cggtgtattt tgcggatcat tgtgcgggaa atagttatac ggtacatccg
    36541 ggtctagtcc ggcttccaca tcgcggaaaa attcctgcgc cagcgtttgc gcatcatatt
    36601 cgggatggcc cgtcacaaag gcaatgcgct tatctttact ggcaaacaga tatgcatccc
    36661 cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac gctgccggaa
    36721 agtcagcata gcgcgaatgc ggtgccagga atgaatcatc aaagccacgc gtcagaagcg
    36781 catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg cgagtttgct
    36841 taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc ccagcagaca aacagcgtcg
    36901 aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag taagcgacat
    36961 cattaaactc caccaggccc agcggcgcac cagttacaat caaaccgtca aagttctgat
    37021 cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc gtgttgcgcg
    37081 attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag tttgaaagca
    37141 ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg atcagaacct
    37201 taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag acgttttctt
    37261 cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aatcggcata acctgattac
    37321 ctcactacat acgcttatac gtttagacat ccagatagct gaagataacc agaaaatttg
    37381 ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaagact acgttagaat
    37441 ataagaatga ctaaaaggag agaaaatggt tattagtatt cgccgctcac ggcatgagga
    37501 aggggaggaa ctcgttgcga tttggtgtcg ttctgtcgat gccactcacg attttctatc
    37561 agcagagtat cggaccgagc tggaggacct agttcgttcc ttcctgccgg aagcgccgtt
    37621 gtgggtcgcg gttaatgagc gggatcagcc ggttggattt atgttgctaa gtgggcagca
    37681 tatggatgcg ctgtttatcg atcctgatgt gcgcggctgc ggcgtaggtc gggtgctggt
    37741 ggagcatgcg ctctcgatgg caccggaact gacaaccaac gttaatgagc aaaatgagca
    37801 ggcggttggg ttctataaga aggtgggttt taaggttacg ggacgctctg aggtggacga
    37861 tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg ggggcgtagg cgattttttc
    37921 tcatcccggt gggctgaact tcaggtttag tacccaccaa tggtgacacg attatttctg
    37981 cttaagcatt gccatgaccg cactatcact gagtaccgct gattgcgcat ggatgtggaa
    38041 ctggcgatat ttcattaacg ttgccccagc gaccacttca tggagaatgc gttcctcaac
    38101 ctgtgacaaa ccgccctcat tcatttccag cgcgctcaca acaatatcca tacgttttgc
    38161 tggcaaaaaa gaatgccagg ttgaacgtgg gtggagttgc agaacaagat cttgcagaac
    38221 tttttctgca acaaagaaat tggcgattaa caggcgctgg ttactgaagg cagcgtctcc
    38281 tgacacttca cgtttgcttt caagatcgcg aacagtaatt tgattacgac gaatttggat
    38341 atacagaact gacatgagat tcccttcatc atgcaaataa ttgatatgca aaaaaccccg
    38401 gccttatgga ccggggtttt t
//
LOCUS       DBBGLQ010000043        37978 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.043, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000043 DBBGLQ010000000
VERSION     DBBGLQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.043"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..675
                     /gene="yghT"
                     /locus_tag="ACIYSB_002809"
     CDS             <1..675
                     /gene="yghT"
                     /locus_tag="ACIYSB_002809"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGC5429826.1"
                     /translation="PLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVGR
                     AVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGL
                     IVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNV
                     DLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFM
                     TARGYH"
     gene            complement(724..2223)
                     /gene="pitB"
                     /locus_tag="ACIYSB_002810"
     CDS             complement(724..2223)
                     /gene="pitB"
                     /locus_tag="ACIYSB_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HGC5429827.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVAAVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2515..4374)
                     /gene="gss"
                     /locus_tag="ACIYSB_002811"
     CDS             complement(2515..4374)
                     /gene="gss"
                     /locus_tag="ACIYSB_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGC5429828.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4579..5445
                     /gene="yghU"
                     /locus_tag="ACIYSB_002812"
     CDS             4579..5445
                     /gene="yghU"
                     /locus_tag="ACIYSB_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGC5429829.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5474..5555)
                     /locus_tag="ACIYSB_002813"
                     /pseudo
     CDS             complement(5474..5555)
                     /locus_tag="ACIYSB_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5568..5816)
                     /gene="hybG"
                     /locus_tag="ACIYSB_002814"
     CDS             complement(5568..5816)
                     /gene="hybG"
                     /locus_tag="ACIYSB_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGC5429830.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5829..6170)
                     /gene="hypA"
                     /locus_tag="ACIYSB_002815"
     CDS             complement(5829..6170)
                     /gene="hypA"
                     /locus_tag="ACIYSB_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGC5429831.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(6163..6651)
                     /gene="hybE"
                     /locus_tag="ACIYSB_002816"
     CDS             complement(6163..6651)
                     /gene="hybE"
                     /locus_tag="ACIYSB_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGC5429832.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6644..7138)
                     /gene="hybD"
                     /locus_tag="ACIYSB_002817"
     CDS             complement(6644..7138)
                     /gene="hybD"
                     /locus_tag="ACIYSB_002817"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGC5429833.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(7138..8841)
                     /gene="hybC"
                     /locus_tag="ACIYSB_002818"
     CDS             complement(7138..8841)
                     /gene="hybC"
                     /locus_tag="ACIYSB_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGC5429834.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8838..10016)
                     /gene="hybB"
                     /locus_tag="ACIYSB_002819"
     CDS             complement(8838..10016)
                     /gene="hybB"
                     /locus_tag="ACIYSB_002819"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGC5429835.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(10006..10992)
                     /gene="hybA"
                     /locus_tag="ACIYSB_002820"
     CDS             complement(10006..10992)
                     /gene="hybA"
                     /locus_tag="ACIYSB_002820"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGC5429836.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10995..12113)
                     /gene="hybO"
                     /locus_tag="ACIYSB_002821"
     CDS             complement(10995..12113)
                     /gene="hybO"
                     /locus_tag="ACIYSB_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGC5429837.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12302..12589)
                     /gene="yghW"
                     /locus_tag="ACIYSB_002822"
     CDS             complement(12302..12589)
                     /gene="yghW"
                     /locus_tag="ACIYSB_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGC5429838.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12708..13592)
                     /gene="yghX"
                     /locus_tag="ACIYSB_002823"
                     /pseudo
     CDS             complement(12708..13592)
                     /gene="yghX"
                     /locus_tag="ACIYSB_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
     gene            13749..14789
                     /gene="gpr"
                     /locus_tag="ACIYSB_002824"
     CDS             13749..14789
                     /gene="gpr"
                     /locus_tag="ACIYSB_002824"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGC5429839.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTKELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14829..15323)
                     /gene="yqhA"
                     /locus_tag="ACIYSB_002825"
     CDS             complement(14829..15323)
                     /gene="yqhA"
                     /locus_tag="ACIYSB_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGC5429840.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15514..16398
                     /gene="yghA"
                     /locus_tag="ACIYSB_002826"
     CDS             15514..16398
                     /gene="yghA"
                     /locus_tag="ACIYSB_002826"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGC5429841.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16670..17095)
                     /gene="exbD"
                     /locus_tag="ACIYSB_002827"
     CDS             complement(16670..17095)
                     /gene="exbD"
                     /locus_tag="ACIYSB_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGC5429842.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(17102..17836)
                     /gene="exbB"
                     /locus_tag="ACIYSB_002828"
     CDS             complement(17102..17836)
                     /gene="exbB"
                     /locus_tag="ACIYSB_002828"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGC5429843.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            18088..19275
                     /gene="metC"
                     /locus_tag="ACIYSB_002829"
     CDS             18088..19275
                     /gene="metC"
                     /locus_tag="ACIYSB_002829"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGC5429844.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19415..20074
                     /gene="yghB"
                     /locus_tag="ACIYSB_002830"
     CDS             19415..20074
                     /gene="yghB"
                     /locus_tag="ACIYSB_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGC5429845.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(20114..21013)
                     /gene="yqhC"
                     /locus_tag="ACIYSB_002831"
     CDS             complement(20114..21013)
                     /gene="yqhC"
                     /locus_tag="ACIYSB_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGC5429846.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            21207..22370
                     /gene="yqhD"
                     /locus_tag="ACIYSB_002832"
     CDS             21207..22370
                     /gene="yqhD"
                     /locus_tag="ACIYSB_002832"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGC5429847.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22475..23302
                     /gene="dkgA"
                     /locus_tag="ACIYSB_002833"
     CDS             22475..23302
                     /gene="dkgA"
                     /locus_tag="ACIYSB_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGC5429848.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23502..24428
                     /gene="yqhG"
                     /locus_tag="ACIYSB_002834"
     CDS             23502..24428
                     /gene="yqhG"
                     /locus_tag="ACIYSB_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HGC5429849.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24479..24736
                     /gene="yqhH"
                     /locus_tag="ACIYSB_002835"
     CDS             24479..24736
                     /gene="yqhH"
                     /locus_tag="ACIYSB_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGC5429850.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24779..26998)
                     /gene="ygiQ"
                     /locus_tag="ACIYSB_002836"
     CDS             complement(24779..26998)
                     /gene="ygiQ"
                     /locus_tag="ACIYSB_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGC5429851.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNHNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(27109..28521)
                     /gene="ftsP"
                     /locus_tag="ACIYSB_002837"
     CDS             complement(27109..28521)
                     /gene="ftsP"
                     /locus_tag="ACIYSB_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGC5429852.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMNDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28596..29333)
                     /gene="plsC"
                     /locus_tag="ACIYSB_002838"
                     /gene_synonym="parF"
     CDS             complement(28596..29333)
                     /gene="plsC"
                     /locus_tag="ACIYSB_002838"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGC5429853.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPTDAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29567..31825)
                     /gene="parC"
                     /locus_tag="ACIYSB_002839"
     CDS             complement(29567..31825)
                     /gene="parC"
                     /locus_tag="ACIYSB_002839"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGC5429854.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(31963..33570)
                     /gene="ygiS"
                     /locus_tag="ACIYSB_002840"
     CDS             complement(31963..33570)
                     /gene="ygiS"
                     /locus_tag="ACIYSB_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGC5429855.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(33703..34098)
                     /gene="mqsA"
                     /locus_tag="ACIYSB_002841"
     CDS             complement(33703..34098)
                     /gene="mqsA"
                     /locus_tag="ACIYSB_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HGC5429856.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(34100..34396)
                     /gene="mqsR"
                     /locus_tag="ACIYSB_002842"
     CDS             complement(34100..34396)
                     /gene="mqsR"
                     /locus_tag="ACIYSB_002842"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HGC5429857.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(34601..35083)
                     /gene="ygiV"
                     /locus_tag="ACIYSB_002843"
     CDS             complement(34601..35083)
                     /gene="ygiV"
                     /locus_tag="ACIYSB_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGC5429858.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(35136..35528)
                     /gene="ygiW"
                     /locus_tag="ACIYSB_002844"
     CDS             complement(35136..35528)
                     /gene="ygiW"
                     /locus_tag="ACIYSB_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGC5429859.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            35680..36339
                     /gene="qseB"
                     /locus_tag="ACIYSB_002845"
     CDS             35680..36339
                     /gene="qseB"
                     /locus_tag="ACIYSB_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGC5429860.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            36336..37685
                     /gene="qseC"
                     /locus_tag="ACIYSB_002846"
     CDS             36336..37685
                     /gene="qseC"
                     /locus_tag="ACIYSB_002846"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGC5429861.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMIVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(37731..>37978)
                     /locus_tag="ACIYSB_002847"
     CDS             complement(37731..>37978)
                     /locus_tag="ACIYSB_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HGC5429862.1"
                     /translation="NKYDWMLESGGNICSIPSVSGEDRILQAMIAAFFLLTPLIILIL
                     RKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         9410 a        10065 c         9504 g         8999 t
ORIGIN      
        1 ccattaattg ccgttattgg tagcgatggt tcaggcaagt caacggtgtg tgaacatctt
       61 attaccgttg tcgaaaaata tggtgctgcc gaaagagttc atttaggaaa acaggccgga
      121 aatgtcggtc gtgcagtgac aaaattaccg ttgatgggaa aatccttaca taaaacaatt
      181 gaacgaaatc aggtgaaaac agcaaaaaaa ttgcctggac cagttccggc gctggtaatt
      241 acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg tcgtcgtcgc
      301 gggttaattg ttctaaccga ccgttatcct caggaccaaa ttcctggcgc ttacgatggt
      361 acggtgttcc cacctaacgt tgaaggtggt cgttttgtct catggctggc aagccaggaa
      421 cgtaaagcgt ttcactggat ggcgagccat aagcctgatc tggtcatcaa actcaatgtt
      481 gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac gggaatcgct ggcgaggaag
      541 attgccataa cgccacagtt aacctttggt ggtgcacaac tggttgatat cgatgccaat
      601 cagccactgg aacaggtgtt ggttgatgca gaaaaagcga ttacggattt tatgaccgcg
      661 cgtggttatc actagtcaaa aatggaaatg cccgatcgcc aggaccgggc attttcagga
      721 aggttaaatc aactgcaatg ctatccagta cagcccacca gaaagaaaaa ttgccgccgg
      781 taaagtaaat acccacgcca tcaggatgct ggttaccgtt ttacgctgta acccaccgcc
      841 gtccaccacc atcgtccctg caactgcaga cgagaggacg tgtgttgtgg agacgggcat
      901 cccaatataa ctggcaagac cgatagacac tgccgccgtc atttgtgccg ccatgccttg
      961 cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta cacgacgcca
     1021 gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc atgatgatcc acaccggagc
     1081 gtactcaatg gtgcttaaca tatcgctgcg aagttttttc agcaggttct ggtcttcttt
     1141 actgacgcct ggcagtttcg ctagcttcgc ggaggtatca gagatgcaca gcataatgcg
     1201 gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat tgcctggcag
     1261 catcgtttta acgcgcgcaa tagcatcaaa ggtatttgcc ggatgacagt gaaactctgt
     1321 tacttgcgtg ccatcagtcg atgctgcagg caatggaggt tccatcgcaa tcaacttctg
     1381 cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg gtaacggcat cgcgggtacg
     1441 ggtaatttca tagccggacg cattcatatt gacgacgaag ccagcagggg caatccccac
     1501 cagtaccagc attaccaggc cgatcccttt ttgtccgtcg ttcgcgccgt gcgaaaacgc
     1561 cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag aatggcggtt tacgtttgcc
     1621 ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt tcgtcccgct
     1681 ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg accaggccga cgatagggga
     1741 aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg tttaacgcat ccatcaccga
     1801 tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcaccaa tcaaggtgtg
     1861 cgaactggag gccggtaaac cgaagaacca cgttcccagg ttccagataa tcgccgccag
     1921 cagcatggaa aagaccatcg ccaggccgtg ggttgacccc atattcagca acaaatcggt
     1981 tggcaacata tggacaatgg cataggcaac gctaagtccg cccaataaca cgccaaaaaa
     2041 gttaaaaaat gccgccatca ccacagcaag ttgtggttgc atggcacgag tataaataac
     2101 ggctgccacc gcattcgccg tgtcatgaaa accattgatt gcttcgtaga acaacacaaa
     2161 tgccagagca agcaataaca aaagccctgt gtatatatca aggccaacaa ataaatttag
     2221 catatattag attacgccat tttgaattta cgaacggacg cattatcagt gactttaacg
     2281 gcatgggcaa agtgaaatat cattttttaa cgataaaaag agtgttattt cttgtgtatc
     2341 agacaataag cattcattca aaacctttag aaataatcaa aacacaggta ttccactggt
     2401 taatatattc acatatgaaa tgaataaata ttggaatata taaatattga atattttgat
     2461 taatccccgt actgattatt cttcataatc agtacggggt tgctacaaca tgtattactt
     2521 tttcaccaca attaacggtt caatatcact ctcttttttg atgaccagtg attcatcacc
     2581 gcgcaaacac gtcccaccgt agttgccgcc aacggtgaag gtacatacct gaatgtattt
     2641 accgtccact ttcggcaaac accacagttg ctgatagatg tttttctgct cggcaaattt
     2701 accgctggtt ttgtccagca cctcttcatg atggctgacg aggtcgatat tgctgccaca
     2761 gcgaccggcg atcggtttca ctgcgtaacc tgttttcacc agttcatcat taacagtgaa
     2821 atcggtatcc agcaggtaac gatggtgcgg gaacagcgac cagaggatcg gcagaatcgc
     2881 tttgttgccg gggatcaccg tccacagcgg ctcaaagacc agcacttccg ggcgcagcaa
     2941 tacgtcgata agacgcactt cgttttgcgg atgaccggta cggattggca ccgcagcaaa
     3001 ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt tcccacgccc aggttttcca
     3061 cacgcagtta accagtcgcc cttccccatc aatcagttgc ccggcagcat cccagcccag
     3121 ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc tggtgcagcg cctgctccat
     3181 aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc atgatatgga caaacggacg
     3241 tgcacgactg tgtttccagg caccagccaa ttcgttaatc agcccttccg ccggattgaa
     3301 gccgttgcct ttatagccct gctccgccca acgttcgagg atcaagcccg cttcggtatg
     3361 acaggaggcg gagtcggcgt tgtactcgta aaccttcagg ccacgctcat ccatgcagaa
     3421 atccatacga ccagtgatca tatggtgacg gcgacgctgc caggagagac gcaaacgtgg
     3481 ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg tcatctttca gcaccttgtc
     3541 ggttgcgtga agatacatca ggtgcagctc gttggtggct ttaattagct cctgctcggc
     3601 actctcggta atggtgtagt aatgataagg atcctgattg atcacctgac cgttggcctg
     3661 cacataggcg ttttgcagcg gatctttttc atccagccat ttaccgtcaa actggccttt
     3721 gttttccagg cgcgctccgc tgattttcag cagctcgcct gcaatttccg gctgcggtaa
     3781 gctgtattca gtatcttccg tctggatcat ccagcccaga atggtggtgt catcaaaagt
     3841 gtctttcagg gtatagcagc cgttttcgac caccatctcc agctcgcgcg tccactgttg
     3901 cccttgcggc aacggggaat gaatcacgtt ctgttccgca atacggactt tgttgccatg
     3961 caattgggta atgatggcga catggccagt gtctttaaat tcaccgcctt tatcccagat
     4021 aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct gcaatggcag
     4081 gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc acgccatacc
     4141 cacgtcagta aagaccacac cgtaattcag aaagagaaaa cggcgagcaa attcaacgca
     4201 ttgccacttg tggcccatat attcgtcgtc gatatagcta cggaatacgg cgtcatcttc
     4261 gtattcctgc ggatcgagag aactgtaatc tgaagagtag attgctaccc cacctggggc
     4321 gtagcccaat aatgtcccga acggggcatc ctggctggtc gttcctttgc tcatcacttt
     4381 acctttaaca atacaaccta agcagttggc gtaattgttg tgctctgatt acctgctcag
     4441 cgacattaac cggacagagg tcagactaat catacacctc atcgcagcgg ctggcgcaaa
     4501 gggttaaaaa attcacattc tgtacagcaa gtgacctgct acactgcttc aacactacca
     4561 ctcagaaggc aactcactat gacagacaat acttatcagc ccgcgaaagt ctggacgtgg
     4621 gataaatccg ctggcggcgc gttcgccaat atcaatcgcc cggtttctgg tccgacgcat
     4681 gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt attcgctggg aacgccgaac
     4741 ggtcagaaag taacgattat gcttgaggag ctgctggcgc tgggcgttac tggtgcagag
     4801 tacgacgcct ggctgattcg tattggcgat ggcgatcaat tctccagcgg ctttgtcgaa
     4861 gtgaacccaa actcgaagat cccggcgctg cgcgatcata cgcataatcc gccgatccgc
     4921 gtgtttgaat ctggttcgat cctgctttat ctggcggaga aatttggcta cttcctgccg
     4981 caggatttgg caaagcgtac tgaaacgatg aactggctgt tctggttaca gggtgcggca
     5041 ccgttcctcg gcggtggttt tggtcacttt taccattacg caccggtaaa gattgagtac
     5101 gccatcaacc gctttaccat ggaagccaaa cgtctgctcg acgtgctgga taagcaactg
     5161 gcgcagcata agtttgttgc gggcgatgag tacaccattg cggatatggc gatttggccg
     5221 tggtttggca acgtggtgtt aggtggtgtg tatgatgccg ctgagtttct tgatgcgggc
     5281 agttataagc atgtacaacg ctgggcgaaa gaagtaggcg aacgtccggc ggtgaaacgt
     5341 gggcgtattg ttaaccgcac caacggaccg ctgaatgagc agttgcatga gcgccatgac
     5401 gccagtgatt tcgagacgaa tacggaagat aagcgtcagg ggtaagggtt ggtgttcgtc
     5461 gcagcaagcc atccaggccg gataaggcgt tcacgccgca tccggcaatc gtgcacaatg
     5521 cctgatgcga cgctgccgcg tcttatcagg cctacgaaag ctaatcatca cgcgctggta
     5581 atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt tggcttcatc ttcgtcgatg
     5641 atgctcatgg caaatccgac gtgtaccagc acccactggc ccagtagatc ggcagggtta
     5701 ccttcacaaa tcagggcgat attcacatcg cgcttgatac cacatacttc aacctgcgca
     5761 agctggtgaa tatcttcacc gacagccagc acctggcctg gaacgccaat acacattatt
     5821 aactccggtt attcaacttc aatacttttg acgatcagcg aatcgccggt atcgacacgc
     5881 aaccgctcgc cgtgacagag cggacactgc gcatcgtgct gatgaatctc caccacctgg
     5941 ctgcaatccc agcaccaagc ctgggcgggt ttatagacga tatgtaaatc gcacccttgc
     6001 gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc ctcaacgcag
     6061 gagagcgcgc caatttccag ccacacggcg gtgacgcgct taacatcgtg ctgctccgcc
     6121 tgccgttgga taatttcaac ggcgctctgg caaagagaca actcatgcat tttcgccact
     6181 cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt acatccggat tcgtgactgg
     6241 cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt tggccctctt caatcgacat
     6301 gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat tgcgaaacac cgtccagttc
     6361 gccaacggta aaggtcatag tgccatacgg cagttgcaga ccaatttttt cactcacttt
     6421 gcgcagcggc cagagttgat ccgggccggg gaagataact gcactcagca tccacggggt
     6481 gatcacacac cccgtccact gaccttcgaa cagcgtaaaa tcagaaacat acaccggcat
     6541 tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt caaacgctgc
     6601 ctgtacttgc gccttcgggg aggtctggaa acctgctatc tcttcagtca tgaatcgcct
     6661 cccgtgggat ggcttccacg ccagattcac gcagcgcagc cagaacctgc tcaagcgcag
     6721 gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg tggctccagc gattccggga
     6781 tcacgccgac cagggtcagc tttttcggaa actcgccggt gaagcgcagg gccgacaaga
     6841 cgtcggccag gccaagctga tgcggagaga ttttgttggt aaacaacgcc ggaacttctt
     6901 catcccgcag gatcatcatc gttcccggcg cgttcttttt cgagacaatg gcatccgcaa
     6961 taatcaaatg atcgcgattt gccatgtcgc caagcagctc cattcccgcc gtgccgccat
     7021 cgaggatctc aacataatcc ggcagaatgt atcgttgctc taacgcttcg acaatccgca
     7081 caccgatggc ttcatcggtc agcaaaatat tgccgacccc taagactaaa atacgcatta
     7141 cagaaccttc actgaaacca cttcgttgcc gtcagcatcc actacgtgta ccgcacaggc
     7201 catgcacggg tcaaaggagt gaatggtacg caccacttcc agcggtttat tcggatcggc
     7261 aaccggtgta cccaccagcg actgctcgta aggaccgacg tcatcattga agttacgcgg
     7321 accagagttc caggttgatg gaacaaccgc ctggtagttg ctgatgatac cgtctttaat
     7381 aaccatccag tgagagagca taccgcgcgg cgcttcgagg aagccaacac ctttgaattc
     7441 acccgttgcc ggaatgttcg gcttcacaaa ggtggtgtga tcgcctttgc cgatattggt
     7501 gatcagtgca ctgtattggt tttgcaggat atcctgcaat tcacagcagt gaacggtacg
     7561 accaataata cggcccagcg tggagtgcag ctgtgccact tccagcgtgt tgccagtcag
     7621 tttctgataa atcgcaacga tttcattcag tttgttctgg gtagattcgc gacctgccgc
     7681 cagtttcacc agcatattag ccagtggccc cacttctacc gttttgccgt agaaagtcgg
     7741 tgatttcacc caggaatatt tcccgtcgtc agaccaacca tcataagccg gaatggtggt
     7801 gccttcccac ggtgcctgcg gcgcttcgtc tttataccag gagtgcttcg cgctttcctg
     7861 aatgcctttg atcaggtatt catcggaatg agaagtgatc ggacgatacg aggacagatc
     7921 cgcattctca atgtagccgc ccgggaacag gaagctgccg tttttactgt cggtcgggaa
     7981 ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgt gtcagccatt ccgggtagaa
     8041 cgcggcaata actgcggtat caaccttata aacctgctca acaaagtcgc tcagtttgtc
     8101 gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc acgcccaaac cgtcgaggtt
     8161 gatcgggttc gcgacaccac ctaccgccag gttctgaatg tgcggcgttt taccgcccag
     8221 cagcgccacg acgcggttag cgtcacgctg gcactccaac gcttgcaggt agtgcgctac
     8281 cgcaatcagg ttcacttccg gcggcagttt catcgccgga tgaccccagt agccattagc
     8341 gaaaataccc aactgaccgc tggcaaccag atctttgatc ttgttctgaa ctttggtgaa
     8401 ctcttccgga ctgttcaggt gccaggtcga aacgcctttc agcatttcgg aggctttggt
     8461 tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa gctgatagaa
     8521 atgaacaata tggtcatgcg tggtgtgcgc agccagaatg atgttacgga tgtattgcgc
     8581 gttaaccgga acgtcgatat tcagcgcact ttctgccgca cgaacggaag acagcgcgtg
     8641 agtggtagta catacgccac agatacgttg cacaatcatc catgcatcgc gcggatcgcg
     8701 gtttttcacg atctcttcca tgccgcgcca catggtaccg gaagcccatg ctttcgaaac
     8761 gacgccattt tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg ttaccggatc
     8821 aatagtaatt ctctggctca tgctttgctc gcctcatgac gattatgatc gttttgtttt
     8881 aaaggaggaa gtatcggcag tagacgaatg agtacgatgt aagcgcaaat ctcaatagcc
     8941 acaaaaccaa tagaaatcaa cagttcttcc caggtcggga agtaggcgta accgccgccc
     9001 gggttgaatg ccaccagcga ataggtcaga cgccaggttg cacaacctaa cagtgcgctc
     9061 agtgctgaca ggaacagcat gcgggaatca ttacgcagct tcgccacacg cagaacgacc
     9121 agcgggaaga gcatcagcag gacttcaatc cagaacatca cggagtagaa gtcaccggca
     9181 aacgctaacg acagcttgtc gcgatagata agctcgccaa agcgcagcac gatgaaaatc
     9241 gccagcaaca cactgatggt gttggtcagc ttaacaaaca gactcttttc atccggaccg
     9301 ttgccacgca gacccgcctg caccagcgaa ccttcaaaga tgacaatcga gaagcccatg
     9361 atgaacgccg tcagcagcga gaacagcggc aacatttcat agctctgcca caacggatgc
     9421 accttgtagc ccgccgagat catcagcgac cccattgaag actggtgcat ggtcggcagc
     9481 agcgcaccga gcgcgatgat gaagaacatc accttgttta gtcgctgtag cgacaccttc
     9541 caccccagac gttcaaacag tgccggagca aactccagtg ccatcacgcc gatatagatg
     9601 gtcatacaga ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc cggaatgtag
     9661 aagtacggca ggttccagta gcgacccacg tcgatagtga tcgacaagcc acccagtgag
     9721 taaccaaaca gactcgccaa cagcgccgga cgcaccagcg gatggtattg cccacggtta
     9781 aagacgtata ccgcccacgc cagcgcccag ccgccacagg caaagccggt gccaatcagc
     9841 aggtcaaacg cgatccacac gccccacggg aagccgccgt tcaggtcaga gacagagccc
     9901 agaccgaaca ccagacgctt cacaatcagg agcatacaga tgacgattaa cggtccaaaa
     9961 atcatgaccg gtttactgat gattttgccg cccagcggtt gtggatcatg actcatgatc
    10021 gtctcctccg tcgtgatggt cgtttttggt gttgcgacga accagcacgg ttaagcccgc
    10081 cagcacagcc agtggtagca tcatgccttt atacagggtg tgttgaatat tttcggaacg
    10141 cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga ttttcataag gcacacccgt
    10201 cagtaccaga acctgagtac cgccgccctc tttctcgccg tacagatgcg gataatattt
    10261 cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat agtggtattc
    10321 gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc atcagctctt cacgcgtacc
    10381 gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca ggtagaccgc ctttatcgag
    10441 acgttccaca cctttctggt tgcacagctc gcacttatgc agcgcaccaa acgggttgtt
    10501 gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg cagtaacggc agccggtgca
    10561 cacatctttg tcgtaatgga caatgccggt tttcggatct tttttcagtg cagagaccgg
    10621 gcacacagag acacagttcg gatcgacgca gtgcatacac tgtttcttaa tgtacgcgta
    10681 gccgttctcc tcctggtctt tgttgacccc tgtgccgctg gtccacacct gaatgatgtt
    10741 attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt gcgggttacg
    10801 ttcagggaaa ttgatatcct gacacttggt gacgcaagcc tggcagccta cgcacaaggt
    10861 cgagtcgtac aacatcccca gcgatcccgg aattggcggg cggttttcag cagccgcatg
    10921 actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt taataaaatt
    10981 acgtctgttc acggttattc tccccgtgag tcagcgttat ctttcttttg ctgacgaccc
    11041 agttcacgca ccgccatcac gctgacaccg gcaaccaacc caaccacacc gccgagcaaa
    11101 ccaatagcgc ctgcagagac gttgccgccc tctttagcgt taacatccgg tttctgtgaa
    11161 cgcggagttt gattttcgac gttggcaagc tgatggatgc ctttatggaa gccgatacct
    11221 tcttcgttac agccatagca cgggtgacca atcgccaccg gccacacacc gccaacatcg
    11281 cagaattgca gcgttgagca gttgccgtaa gtttctggcc ctttacagcc gaggtggtac
    11341 aggcaccagc cttcgcggtg gccttcatca ccgaactctt tggcaaaacg accagcatcg
    11401 aagtgcgggc gacgttcgca gtgttcgtga atcagacggc cataggcgaa ggtcggacgg
    11461 tttttgtcat ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac ggtcgcgagg
    11521 aagttgtgcg ggttcggcgg gcagcccgga atattgataa cggttttgcc tggcagaact
    11581 tcttgcaggc tgactgcgcc agttgggtta actccagctg cggcaacacc gccccacgca
    11641 gagcaggaac cgatagcgat aatggctgct gcgccttccg ccgctttgcg gatgtgatcc
    11701 acaatcggct caccggcaac catgcaataa ataccgttat cttttaatgg gatggaacca
    11761 tccaccacta acacatactg ccctttgtac ttctcgagag cgttatgttt gttctcttcg
    11821 acctgatgac cgaaggcggc ggaaagcact tcgtgatact ccagagagat agtctccagt
    11881 acgaggtttt ctaccgttgg atgcgttgca cgaagcagag attccgtaca accggtgcac
    11941 tcctgcgcgc caatccagat aactggcgga cgctgcgggt tagtaaccga ttcggccatc
    12001 tctgcagcgg ctttgctact taaccccatg gtggcggcta atgctgcaca aagcttcatg
    12061 aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt tatctccagt catttatagt
    12121 tattccgttg cgaagacctg gcatatattt tgcctcaatc gcaaaatcaa taatgcgatc
    12181 gatgcgccat ttaccacaca tttattatgg ttatcgatat catgatactg cggcggggcg
    12241 ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag caagataata cgtatgtttg
    12301 atcaattttc gaggcgataa ccggccataa aaaagcgcaa cgtagaacag gaattattct
    12361 cacgaaagaa atccattacc atctctttat ccagtccata gcggcgcgtc agaatacccg
    12421 cttcccaggc gctaagctgg cgatctccgg tcttttcgta catccggtgt gagtagccta
    12481 atacaaaacc gcgtttataa tcggcacaga attttgtcac attaaccgca ctgtcggcat
    12541 gcgttgcttt taatcccgcc attaagcctt taccaaaatg gttattcatc gttcacctca
    12601 ttcgctatat attgtcatat atagcgatat tttaggcaaa gaacgacttt tgcgcacgct
    12661 attttttcat gcttgtttaa gacgtaatcc cgtaagagat aaacctatca ggagagatat
    12721 ttatcgaacc atttcagtgt cctttgccag gcaagatcgg cggcagattt gtcataacgg
    12781 ggcgtggaat cattatggaa tccgtgatta acccccggat agatatacgc ctcataaacc
    12841 ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc cctcgttgat tcgggtgtcc
    12901 agttccgcga agtggagtag taaaggcgct tcaatcttcg ccacatcggc agtgggtgcc
    12961 tgacgaccat aaaacggcac cgcgcaggcc agttccggat acgcgacagc cgccgcgttc
    13021 gatacgccac cgccatagca aaatccggta atacccactt tgcctgtcgc ttgcggatag
    13081 cgttgcataa actcaattgc ggcaaagaaa tcattcatca gtttgttgga tcaacctgct
    13141 gttgcagctc acgaccttta tcatcatttc ccggataacc tccaacggaa cttaagccgt
    13201 caggtgccag ggcgatatac cccgccttcg ccactcgccg tgccacatct tcgatatacg
    13261 gattcagtcc acgattctca tgcaccacca ctacggctgg cgttttgccg ctcatctttg
    13321 cgggcttcac cagataaccc cgcacctcgc cgtgaccatt tggcgaagga tacgtgatgt
    13381 actcagcaac aatttccggg tcggtaaact ctacctgagt cgccagcgca taatttggct
    13441 tgagcaaatc aaacaacgct aatgccgtca tcccgcctac cgcacttcgc cgcaaggttg
    13501 aggaactcac gtttcgagat tttcccgtga gcgtaatagt catagtaatc cagcaactct
    13561 tgtgggaaat ctttggcggt taaacgcggc atcgttgcac tcctcagttg gtgttttttt
    13621 aagcaaagca taagcacgtg tttttgccca gtttttcgtc actctgtgag ccagactacg
    13681 ggatacgcgc tggcgaatcg ctaaactaga aacattgttt cgaaattgaa cggtggaaag
    13741 gagaggtcat ggtctggtta gcgaatcccg aacgttacgg gcagatgcaa taccgctatt
    13801 gcggaaaaag tggtttacgc ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc
    13861 acgttaacgc gctggaatca cagcgtgcga tcctgcgtaa agcgtttgat ttgggcatta
    13921 cgcactttga tttagccaac aattacgggc cgcctccagg aagcgcagaa gagaactttg
    13981 gtcgcctgct gcgggaggat tttgccgctt atcgcgatga actgattatc tctaccaagg
    14041 ctggctacga tatgtggccc ggcccttacg gctctggcgg ttcacgtaaa tacctgctcg
    14101 ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta tgtcgatatc ttttactctc
    14161 atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc tgcgctggct catgcggtac
    14221 aaagcggtaa ggcgctgtat gtcgggatct cctcttactc gccagagcgg acgcaaaaaa
    14281 tggtcgagtt gctgcgcgag tggaaaattc cgctgttaat tcatcaacct tcgtacaatt
    14341 tactgaaccg ctgggtggat aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg
    14401 gctgtattgc ctttactcct ctggctcagg gattgctgac cggaaaatat ctcaacggca
    14461 ttccgcaaga ttcacggatg catcgtgaag ggaataaagt tcgtggtctg acaccgaaaa
    14521 tgcttaccga agccaacctc aacagcctgc gcttattgaa tgaaatggca cagcagcgtg
    14581 gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa agatgatcgc gtgacgtcgg
    14641 tattgattgg tgccagccgc gcggagcaac tagaggagaa cgtgcaggcg ctgaataatc
    14701 tgacatttag caccaaggag ctggcgcaga ttgatcagca tatcgccgat ggcgagctga
    14761 atctgtggca ggcgtcttcc gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc
    14821 gaatcagatc agtgattatg acgagtcagt cggtcaagat agcccatcac aaatgcagaa
    14881 agcacaaacg tcagatggat aatgacgtac cacatcagtt tgttatccgg gacatttttc
    14941 gcatccataa agacgcgcag taagtgaatg gaagaaattg ccacaatcga cgctgctact
    15001 ttgtttttca gcgacgttgc gtccattttc cccagccagt tcagcttctc tttgttctcg
    15061 gagatatcca gctgtgagac gaaattctca taaccggaaa acatcaccat caccagcaaa
    15121 ccgccaacca gcgtcatatc caccagcgac agcaacacga ggatcaaatc tgattccgcc
    15181 atcgagaaga tattcggcag tacgtgaata atctcctgga agaacttcag cgccagggca
    15241 actaacgcca gcgaaaggcc aaagtacacg ggggcaagca gccagcgaga agcatacatt
    15301 gcattttcaa gaaaacgttc catacattcc tgtcgtgtag ttaaaacggg caacagtata
    15361 tcgcaattga gtaaacagca cgcaacagca aatcaacata tacgttaaac ctttgtcgca
    15421 taattctgag gtttagggat cttcctggtt cccttatcaa cgcatcgaga acgtctaccc
    15481 ttaaaggacg ttgaacctga agggagaaaa acgatgtctc atttaaaaga cccgaccacg
    15541 cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat ccaggcgaag
    15601 atgacaccgg taccggattg cggcgagaaa acctatgttg gtagcggtcg cctgaaagat
    15661 cgtaaagcac tggtgacagg gggcgattcc ggaataggtc gcgctgccgc catcgcttac
    15721 gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga agacgctcag
    15781 gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc aggcgattta
    15841 agcgatgaga aatttgcccg ttcgctggtt cacgaagcgc acaaggcgtt aggcgggctg
    15901 gatattatgg cgctggtcgc cgggaaacag gttgccattc cggatattgc agacctcacc
    15961 agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg gctaacccag
    16021 gaagcgatcc ccctgctacc gaaaggtgca agtatcatca ccacttcgtc aatccaggca
    16081 taccagccaa gtccgcattt actggactat gcggctacga aggcggcgat tctgaactac
    16141 agccgtggct tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca
    16201 ggcccgatct ggacagcact gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag
    16261 tttggtcagc aaacgccgat gaaacgtgcg gggcaaccgg cggaactggc ccctgtatat
    16321 gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg cgtgtgcggc
    16381 ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa
    16441 ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaacgcat gaagattcaa
    16501 catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt gagcacctaa
    16561 tgccggatgc gtcgtgaacg ccttatccgg cctacaaacg cacgaagatt caacatgttg
    16621 caatagattt tgtaggcctg atgagcgtag cgcatcaggc aattctactt tacttcgctt
    16681 tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc agcgtatcca
    16741 ttaccttcat caacgtctcg taatcgacgg ttttgtccgc tcggaagaag atggtggtgt
    16801 ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat
    16861 cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt ttttccgggc
    16921 gcggctgcgg cgtgctggta gaagcaggca agttcacctt cacatctacc gtcgctaacg
    16981 gtgccgccac cataaagata atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt
    17041 tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt gccatcggat
    17101 attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca
    17161 gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc agcatcgctt
    17221 taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga atcgctgcca
    17281 cgaggccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg actgccaggt
    17341 tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag accgtaccaa
    17401 acagaccaac gaacggagaa atcgcgccga tggttgccag atagccgtta ccgcggccca
    17461 tttgacgacc cactgcggcg acccgacgct ccagacggaa gctggtacgt tctttaatac
    17521 cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat
    17581 gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg tttaaggaac
    17641 gcgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgattg aagaactcta
    17701 cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc ccaatcatca
    17761 cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg tccgtctgca
    17821 ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc gcataataat
    17881 atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg ccgtaccttt
    17941 gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt
    18001 ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag tttagacatc
    18061 cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac tcaactggtg
    18121 aatgcaggac gcagcaaaaa atacactctc ggcgcggtaa atagcgtgat tcagcgcgct
    18181 tcttcgctgg tctttgacag tgtagaagcc aaaaaacacg cgacacgtaa tcgcgccaat
    18241 ggagagttgt tctatggacg gcgcggaacg ttaacccatt tctccttaca acaagcgatg
    18301 tgtgaactgg aaggtggcgc aggctgcgtg ctatttccct gcggggcggc agcggttgct
    18361 aattccattc ttgcttttat cgaacagggc gatcatgtgt tgatgaccaa caccgcctat
    18421 gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac gacatcatgg
    18481 tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac taaaatcgtg
    18541 tttctggaat cgccaggctc catcaccatg gaagtccacg acgttccggc gattgttgcc
    18601 gccgtacgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg ggcagccggt
    18661 gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaagccgc caccaaatat
    18721 ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg ttgctgggag
    18781 cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac cgcctatata
    18841 accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga aagcagtctg
    18901 aaagtggctg aatggctggc agaacatccg caagttgcgc gagttaacca ccctgctctg
    18961 cctggcagta aaggtcacga attctggaaa cgagacttta caggcagcag cgggctattt
    19021 tcctttgtgc ttaagaaaaa actcaataat gaagagctgg cgaactatct ggataacttc
    19081 agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct ggcaaatcaa
    19141 ccagaacata tcgccgccat tcgcccacaa ggcgagatcg attttagcgg gaccttgatt
    19201 cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga cgccggtttt
    19261 gcgcgaattg tataacattg ccacttttgg acaattttgc agacatttta ttgtgaaaag
    19321 tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta caataggcag
    19381 ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag atatcatcgc
    19441 tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta gcgttgttta
    19501 ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct catttttgcc
    19561 aggcgacagc ttgttgatac tggcaggcgc attgattgcc cagggggtta tggattttct
    19621 gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa gttatattca
    19681 ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa
    19741 atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc tgctggctgg
    19801 acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt ccggtctgcc
    19861 aaaccgccgc ttccagtttt tcaactggtt aagtggattg ctgtgggtca gcgtggtaac
    19921 cagttttggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag atcaggtaat
    19981 gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag gcacgctgtt
    20041 tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc cggttgctgt
    20101 accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct tcccccggcg
    20161 tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca tagccgacgc
    20221 gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc gccttatgca
    20281 gacggtaatt cttcaaatac tgcaacggcg aggtactggt gacagactta aaattatggt
    20341 ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc aggttttcgg
    20401 tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag actgaagtga gtctggcgac
    20461 tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac agaatttcgc
    20521 ggatgatctg tttgccgaga atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc
    20581 gctccgccgc gcataaaatc tcttctgata acgtggcgga gttaatcccg ctggctgcca
    20641 tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa
    20701 tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag gtttcgcact
    20761 caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga aacacgcgtt
    20821 cattgatata accgatttta tgcccggaaa agagaattat gatgccaggc tcgtacatca
    20881 ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc aacagttctg
    20941 acaaactatt ttctttattt ttcagtttat taactttatc cgccagcaag cggcaaatct
    21001 cttcacgttt catatcgcgt aatttcttag gaataatgcg gcaatttgat tgtgcgcaat
    21061 tttgtagcat ttctccagca ctctggagaa ataggcaaga cattggcaga aatgagcatt
    21121 gagagccagg gcgctggcga tcacaatgaa aaacatcagg cagatcgttc tctgccctca
    21181 tattggccca gcaaagggag caagtaatga acaactttaa tctgcacacc ccaacccgca
    21241 ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca aattcctcac gatgctcgcg
    21301 tattgattac ctacggcggc ggcagcgtga aaaaaaccgg cgttctcgat caagttctgg
    21361 atgccctgaa aggcatggac gtgctggaat ttggcggtat tgagccaaac ccggcttatg
    21421 aaacgctgat gaacgccgtg aaactggttc gcgaacagaa agtgactttc ctgctggcgg
    21481 ttggcggcgg ttctgtactg gacggcacca aatttatcgc cgcagcggct aactatccgg
    21541 aaaatatcga tccgtggcac attctgcaaa cgggcggtaa agagattaaa agcgccatcc
    21601 cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga atccaacgca ggcgcggtga
    21661 tctcccgtaa aaccacaggc gacaagcagg cgttccattc tgcccatgtt cagccggtat
    21721 ttgccgtgct cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg gctaacggcg
    21781 tagtggacgc ctttgtacac accgtggaac agtatgttac caaaccggtt gatgccaaaa
    21841 ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat cgaagatggt ccgaaagccc
    21901 tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg actcaggcgc
    21961 tgaacggttt gattggcgct ggcgtaccgc aggactgggc aacgcatatg ctgggccacg
    22021 aactgactgc gatgcacggt ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac
    22081 tgtggaatga aaaacgcgat accaagcgcg ctaagctgct gcaatatgct gaacgcgtct
    22141 ggaacatcac tgaaggttcc gatgatgagc gtattgacgc cgcgattgcc gcaacccgca
    22201 atttctttga gcaattaggc gtgccgaccc acctctccga ctacggtctg gacggcagct
    22261 ccatcccggc tttgctgaaa aaactggaag agcacggcat gacccaactg ggcgaaaatc
    22321 atgacattac gttggatgtc agccgccgta tatacgaagc cgcccgctaa gctttttacg
    22381 cctcaaactt tcgttttcgg gcatttcgtc cagacttaag ttcacaacac ctcaccggag
    22441 cctgctccgg tgagttcata taaaggagga acgtatggct aatccaaccg ttattaagct
    22501 acaggatggc aatgtcatgc cccagctggg actgggcgtc tggcaagcaa gtaatgagga
    22561 agtaatcacc gccattcaaa aagcgttaga agtgggttat cgctcgattg ataccgccgc
    22621 ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga
    22681 agaactgttc atcaccacta agctgtggaa cgacgaccac aagcgccccc gcgaagccct
    22741 gctcgacagc ctgaaaaaac tccagcttga ttatatcgac ctctacttaa tgcactggcc
    22801 cgttcccgct atcgaccatt atgtcgaagc atggaaaggc atgatcgaat tgcaaaaaga
    22861 gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc aacgcctgat
    22921 tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa cttcatccgc tgatgcaaca
    22981 acgccagcta cacgcctgga acgcgacaca caaaatccag accgaatcct ggagcccatt
    23041 agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg cagataaata
    23101 cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg gatagcggcc tggtggtgat
    23161 cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt gatgtctggg atttccgtct
    23221 cgacaaagac gaactcggcg aaattgcaaa actcgatcag ggcaagcgtc tcggtcccga
    23281 tcctgaccag ttcggcggct aacatgcaaa ttctcccggt ggcggtaatg ttccgctacc
    23341 ggacttttca gaaatcattt attcccctcg cgtcccgccc gttgttactc ttccttgttc
    23401 aggaatgcca aatataagga catcatcatg cagagccgga agctcttaaa agaacaactc
    23461 atctatatcc gggataaacg caacggagag gtgaaaaaca gatgaaaata atacttctgt
    23521 ttttagcagc cctggcaagt tttaccgtac acgcacagcc cccctcacag accgtagaac
    23581 aaacagtccg gcatatttat cagaactata aatcagatgc cactgcccct tattttggtg
    23641 aaaccggaga gcgggcgata acttctgcgc gtattcaaca ggcgcttacc ctgaacgaca
    23701 atcttacgct gccgggcaat attggctggc tggattatga tccggtttgt gattgtcagg
    23761 attttggcga tctggtgcta gaaagcgttg cgataaccca aactgacgcc gatcatgccg
    23821 atgccgttgt gcgctttcgt atctttaaag atgataaaga aaagaccacg cagacactga
    23881 aaatggtggc ggaaaatggt cgttgggtca ttgacgatat tgtcagcaat catggcagcg
    23941 tcttacaagc agttaatagc gagaatgaaa aaacgctggc cgctttagct tcgttgcaaa
    24001 aagaacagcc ggaagccttt gttgccgaac tctttgaaca tattgctgat tatagctggc
    24061 cgtggacgtg ggtggtttcc gactcttacc gccaggcggt taatgccttc tataaaacca
    24121 ccttcaagac ggccaataat cccgatgaag atatgcaaat agaacggcaa tttatttacg
    24181 acaatccgat ctgttttggc gaagagtcgc tattttcacg cgttgatgaa attcgagtcc
    24241 tggagaaaac cgccgattcc gcccgcattc atgttcgttt tacgctgacc aatggcaaca
    24301 acgaagagca agaactggtt ttacagcggc gcgaaggcaa gtgggaaatc gctgatttta
    24361 tccgcccgaa cagcggcagc ctacttaagc agattgaggc aaaaactgcc gccagattaa
    24421 agcaatgagc tgaattaaat aacaattagc cggaacaata aataaaaggg aacactatat
    24481 gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc tgcttgctca gtggctgcgt
    24541 caatgagcaa aaggtcaatc agctggcgag caatgtgcaa acattaaatg ccaaaatcgc
    24601 ccggcttgag caggatatga aagcactacg cccacaaatc tatgctgcca aatccgaagc
    24661 taacagagcc aatacgcgtc ttgatgctca ggactatttt gattgcctgc gctgcttgcg
    24721 tatgtacgca gaatgataaa aaaatccccg gtagcgtgtc agttgccggg gatatttttt
    24781 aacgtccaac cgccgcttta ggccgtttct tcgcaccagc attcaccgga cgagattgcg
    24841 tagacgacgc tttttttgcc gtagcaggcg tctgacgctg ggtcgccatc ggcgtatgtt
    24901 tcgtcaacgc cggacgggta ttgtggttct ggcgacgagc ttcacgcatc tcttcaatgg
    24961 ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag atgctttttg cccatcgctt
    25021 ccagcgcctg gcggattaac ggccagtttg ccggatcgtg gtaacgcaac aacgctttat
    25081 gcaaacgacg ctgtttgtcg cccttcggta cgaagacgtc ttcgctctta taaccaatct
    25141 tcgccagcgg gtttttcccg gtgtaataca tggtggttga gttcgccagc ggcgacggat
    25201 agaagttctg cacctggtcg aggcggaagc ggtgcttttt cagccacagc gccagattca
    25261 ccatatcttc atcacgcgta ccggggtgcg cggagatgaa ataggggatc agatactgct
    25321 ctttacctgc ctgttttgag taagtatcga acagctcttt aaagcggtca tagctgccca
    25381 tgcccggctt catcatcttc gataacggcc cttcttcggt atgttccggg gcaatcttca
    25441 gataaccgcc gacgtgatgg gtcgccagtt ctttgatata gcgcggatct tctacggcta
    25501 tgtcataacg cacaccagag gcaatcagga tctttttaat gcctttcaga tcacgcgcac
    25561 ggcgatagag gttgatcgtt ggttcgtggt tcgtgtccat gtgcggacaa atatccggat
    25621 aaacgcacga caaacggcga caagtttgtt cagcgcgtgg cgatttgcag cgcaacatat
    25681 acatgttggc agttggccca ccaagatcgg aaatcacgcc cgtaaaacct ggaacggtgt
    25741 cgcggatcgc ttcgatctca ttaatgatcg aatcttcgga acggctctga ataatgcgcc
    25801 cttcgtgctc ggtgatagaa cagaaagagc agccgccaaa gcagccacgc ataatgttga
    25861 ccgaaaaacg gatcatttcg taagccggaa tacgggcatt gccataggcc ggatgtggca
    25921 cgcgcttgta tggcagcgca aaaacgctgt ccatctcttc ggtagaaagc ggaatagcag
    25981 gcgggttgat ccacacatag cggtcgccgt gtttttgcat caatgcgcgg gcacagcctg
    26041 ggttggtttc gtggtgcaga atacgcgaag catgggcgta cagcacttta tcgcccttca
    26101 ctttctcgaa agaaggcagc aacacgtagg ttttttccca cggtttcggg cgcggtggct
    26161 gcacggttac ggctttggct tcctgctttt tcggtgccac cggtttgtta tccgcgcacg
    26221 gcaaatcttc accatacgga tgcgggattg ggtcgatttt tccaggggta tcaagacggg
    26281 tggaatccac gccgctccag ccaggcagcg cctctttcac gataatcgcg gtattacgca
    26341 catcgcggat ttcactaatt ggctcgccca tcgccagacg atgcgccact tccaccagcg
    26401 gacgctcacc gttaccaaac atcagcatgt cggctttcga atccaccagc acggaacggc
    26461 gcacggtatc ggaccagtaa tcataatgcg cggtacggcg cagactagcc tcaataccgc
    26521 cgaggatcac cggtacatct ttccacgcct ctttacaacg ctgggtataa accagtgtgg
    26581 cgcgatccgg gcgcttaccc gcgacgttat ccggcgtgta ggcatcgtca tgacgtaaac
    26641 ggcgatcggc ggtataacgg ttgatcatcg aatccatgtt gccagcagta acaccgaaaa
    26701 acagattcgg tttacccaga cgcataaagt cgtctttgct gctccagtcc ggctgggcga
    26761 tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc gcaaatcgcc atcccgaagc
    26821 ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa
    26881 gttgatccat ctcttcacga gacatcggca aaaacggtgc cggtccaaaa caggcggccc
    26941 agtactgcgg ccaggagaac aggtcgcgat ccggttggat cagggagata gagctcattt
    27001 tgcttccaga aatgataaaa aaataatcaa aggccgggga ttataagccg gaacgaaaga
    27061 gaaatcgaaa ggtattccat actcgccctc ctcgggcgag tatgaagatt acggtaccgg
    27121 attgaccaac agttgcccaa tcgagccacg gtccgccatt tccagcgtct gactgttgaa
    27181 gtagaacggg aagtgcgccc aggaaggctg accgaaataa acaagcagct ccacttgtcc
    27241 gtcaacccaa acggtatctt tccagcctct gtcttccggg aacggcatcg cgccattcac
    27301 gttacggatc tggaacatta cgccttcaat atggaacgct tgcggctcgt ccgcgcgtac
    27361 cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca atacggttga cgtcccacag
    27421 ctgtccatta atacccgggt catcacccag actgatatcg cgactgcgaa ttggcgaacc
    27481 agccatgatt tcagttggca gcaagcgcat cggaagactg tctgtgacca gcggcagaag
    27541 gccggttggg cgcagcgtta gcaccagggt agaaaccaga atactggatg gctcaaagaa
    27601 gccacgaata cgatcaacaa tgctcgccgc ttcgccacag gtgatcgaca cttcatcgcc
    27661 gttgctcata tccaccagaa tctcgcggcg ctcgcccggt gccagcgaaa gttgcttcac
    27721 cgatacagga gcaggcagga atccctgatc gccagaaatc acatgtaacg ggcgaccatc
    27781 gttcatctgt agttgatagc gacgagagtt cgacgcgttc agcagtcgca agcgcaccca
    27841 gccacgcgag acttcaacgt acgggctttg tacaccgtta accagcagcg tatcaccaac
    27901 aaagccgccg cttcccggtt cgttgtattc tggcgtacca aagttatcca gccgtttatc
    27961 ctggataatg accggaaaat catccacacc ataatggttg gggataggca gcgacttgct
    28021 gacttcatct tccaccagcc acattccggc aaggccgtta tagacctgct gagccgtgcg
    28081 gttgggagta ttggcgtgat accacagagt agctgcgttc tggcgaatgg gcagtacggg
    28141 tgcccagtca gcgtttggcg acatcatccg tgccggaccg cccatcagcg ggcctggtac
    28201 ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg cggttgctgt aaataagctt
    28261 aacatcgtcg cccttccaga cgcggatagt cggccccagg taacgaccat tgattcccca
    28321 gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag tcataaacag
    28381 cggttgccca cggcgagatt caagtagcgg cggaacgggt agcggttgct gttgcccggc
    28441 tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca atccccgatg cctgaatgaa
    28501 ctgacgccga ctgagtgaca tatttgctcc atgtaaaact ggctaaagta ataccgtgca
    28561 ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa cttttccggc ggcttcgcgt
    28621 tctgcgactt ctttatcgag ctcggcgatt ttttgttcca ttatcgaacg acaatgggca
    28681 gccagctcac gaacctgatc tttgccatac tgactgacgt caattggcgg cagcatttcg
    28741 acaatcacca gaccgttgtg cagtcgatta agattaatct tattcgaagt tgtagagacg
    28801 cacacgggaa taatcgggac gcccgccgca attgccgcgt gaaatgctcc agtcttgaac
    28861 ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga acatccagat ggaaatacgg
    28921 cgttttttga agtgattcac tacttccgca atggtgccgt gagctttagt gcgattgttt
    28981 ctgtcgatca ataagttgcc ggttaaccag tacaactgcc cgaagaaggg gatccacagc
    29041 aagctctttt tacctaccgt caccgtcggc ggttgcacga tgttcgatgc tgtcaccatg
    29101 tcatagttgt tctggtggtt agcgatatag atagcattgc cgtagctttc agcgtctgta
    29161 ggtttacggc actcaacttt caggccaaac agcggcgcaa gacggccaaa catatgccca
    29221 aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga gccgaataca
    29281 cagactaaga tgctgtaaat cacggtaata ataagacgaa agatatatag cataacaccc
    29341 tctgaaggtc tgagagactg gcattgtacg tcacctgaaa tcaggttagg gcattgttaa
    29401 aagccgctct ctggaaaagc gtattgttaa tcgcaacgca tgaataaaca acggtttcaa
    29461 gggaattttt atcgtttccg ggggttgaat acgctgccgg atgacgactt aacgtttcat
    29521 ccggcgttcc ttgcaagcgg gaggaaacag cgccctcccc ggcatattac tcttcgctat
    29581 caccgctgct ggcacggcga ggagagtcga tctcaacacg atcgatacgc tgcaaaccgc
    29641 gcatcaacgt accgcggcgt ccacgttcgc cagtgacttt ctgtaactct tccgggcgca
    29701 gtttaatttt gcgtttccca acatgaatgg tcagcgtgct ttgcggcggc agaacgtaca
    29761 attgcgccag accatcttct ccacgcgcgg cttctgccga tggaatgttg ataatcttgt
    29821 tgcctttgcc cttcgacagc tgcggcagat cacttaccgg gaacatcaac atacggcctg
    29881 cctgagtgat tgccagcagc atatcggaag catcttcaat caccaccggc ggcataacat
    29941 gggcattttc cggtaaggtg atcaaagcct tacctgcacg gttacgcgcc accagatcgt
    30001 taaaggtgca gacgaaaccg taacccgcat cggaagccat cagcagtttc tgatcgtcgc
    30061 tttccatcag catatggtca acggtcgccc caggcggcaa cgttaatttg ccggtgagcg
    30121 gctcgccctg accacgcgcc gacggcagcg taatcgggtc aatggcatag ctacgaccgg
    30181 tggaatcaac aaacactacc ggttggttgc tcttaccttt caccgccgct ttgaagctat
    30241 cacccgcttt ataattcagg cccggcgcgt cgatatcatg gcctttagcg ctgcgtaccc
    30301 agcccatctg cgacagcaca atggtgacag gttcagacgg cagcatgtcg tgctcgctca
    30361 tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg atcgtcaccg taggcttgcg
    30421 cgtctgcctg cagttctttc ttcagcaggt tattcatttt acgctcggaa gccaaaatgc
    30481 cctgcaactg gtcgcgctct ttttccagtt cactctgctc accgcgaatc ttcatctctt
    30541 ccagtttggc aagatgacgc agtttcagtt cgaggatcgc ttccgcctgg gtttccgtaa
    30601 ggccaaaccg cgacatcagc gccggtttcg gttcatcttc attacgaatg atctcaatca
    30661 cttcgtcgat attgagaaac gccaccagca aaccttcgag gatatgcagg cgcttgagga
    30721 ctttctccag acgatagttc agtcggcggc gcacggtatc gcggcggaac accagccatt
    30781 cggagaggat ttccagcagg tttttcaccg ccggacgacc atccagaccg atcatattaa
    30841 ggttaatacg atagctcttt tccagatcgg tggtagcgaa gaggtggttc atcacctgat
    30901 ccatatccac gcggttggaa cgcggcacaa tcaccaggcg ggtcgggttc tcgtggtcag
    30961 attcatcgcg cagatcgtca accatcggca gctttttgtt gcgcatttgc gcagcaattt
    31021 gctccagtac gcgcgcacct gaaacctgat gcggcaatgc gctgataacc accgcgccat
    31081 cttctttctt ccacaccgcg cgcatacgca ctgaaccacg tccgttctcg tagattttac
    31141 ggatctcggc gcgcgaagtg ataatttccg cttcagtcgg ataatccggc ccctgcacga
    31201 tatccagcag ctgatcgagc gtggttttcg gctggtcgat taatgcgatt gccgcctgag
    31261 ccacttcacg caggttatgc ggtggaatat cggtcgccat gccgacggca ataccggtgg
    31321 tgccgttaag caaaatgttt ggcagacggg caggtagcat tttcggctcc tgcaaagtgc
    31381 cgtcgaagtt tggcacccag tcagccgtcc cctgccccag ctcgctcaat agcagctcgg
    31441 aatatttcga caaccgggat tcggtgtaac gcattgccgc gaacgatttc ggatcgtccg
    31501 gcgcgcccca gttcccctga ccatcaacca gcggataacg gtaagagaac ggttgcgcca
    31561 tcaggaccat cgcttcataa caggcgctat cgccgtgcgg atggtattta cccagtacgt
    31621 caccgacggt acgggccgat tttttaaatt tggcgctggc attcaggccc agttcagaca
    31681 tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc accaataaac ggcaacgcac
    31741 ggtccatgat cacgtacatg gagtagttta agtaggcgtt ttccgtaaat tcatgtagcg
    31801 caaggcgctc tgccatatcg ctcattaatt ctgattcctc aacttattcg ccagccttca
    31861 aaggggcaat attgccggag atactacatt atctgccgcg tcgagtcaca ggagatcagc
    31921 gtgaatagcg ccgccggacg tgctccgacg gcgataaagg catcaatgtg ccttgatata
    31981 caactctttg ctgtagacat aatcctgggg attatgcagc ggaaaaccgc caacgtaggg
    32041 tttaagcagt ttgattaacg gctgatagta gataggaatc agcggtgcct gttggttgat
    32101 gatcacttct gcctgctgat acaacgcatt acgctttgtc gcatcagtga tctgcgtggc
    32161 ctggtttagt aaggcgtcat actgcgcatt tttccagtga ccgacgtttt cttcactatc
    32221 gcttttgagc gtgttcagga agctggaagc atcattgtac gtcgcatccc acgactgccg
    32281 agacagcatg aaatcaccgg ctcgtcgggc atcaagatag gttttccact ccattgtgcg
    32341 cagcgtcacc tgtgcaccca gccatttttt ccattcggaa gacaacgcta tcgcggtctt
    32401 ttcatgcaga tcgtacttgt tgtagaacag ctcaaagcgt agcggatgag aggcgtcgta
    32461 tcccgcctgt ttcagcaagg cttttgccat cgcgacgcgc tcactcattg gcttttgcag
    32521 ttcatcgaac gtcgtcgcgc taaagccttt tacctctggc ggcgtcagcg tggttgcggg
    32581 cgttctcaac cccagtacct tttgcgcaat aagctgtcga tcaaccgtaa gatatagcgc
    32641 ccgacgcact cgcacatcgt taaatggcgg tttctcaagg ttgaagttgt aatattcgct
    32701 gttcagacgc ggaataattc gtagctcgcc aggcagtgat ttttcaatgg cgggaatttg
    32761 ctgcgccgga acccaggtga gatcgacctc tcccgcgcga tagcggttat agccggtgac
    32821 cgaattatct agcgccagat actcaacctg ttgcaatact gtatgttgcg catcgcggta
    32881 ctttggattt ttgcgtgcag taatcttttc gttaactacc cactgatcaa gcacaaaggc
    32941 accgttgtaa accatgttct ctggcttact ccagctatcg ccatgtttag cgatgacatg
    33001 atgaggaacc gggaacagcg tcggccaggc gagcatcgtc gtgaaccaag gaaccggctg
    33061 ctcaagcgta acttcaagag tacgatcatc cgtcgctttg acacccagcg atgtaacatc
    33121 tgctttaccc gcaacaatag ctgcggcatt gttaatgtgc gcctgtgcca gatagccagc
    33181 aaaagggctt gccgttttcg ggtcaaccgc gcgctgccag ccgaggacaa aatcctctgc
    33241 cgtcagaggc tgaccgtcgg accactgcaa accgctacgc agatggaaaa tatagcgctt
    33301 gccgccgtcc agtatctccc agcgttcagc ctgagcgggc tgcacctggc cttcaccgtc
    33361 catccatacc agaccttcaa acagatccag cacaatctgc gcggcagtat tctcctccac
    33421 cttttgcggg tcgagcgtac ctgggtcgct atgattgttg taacgaaaga cttgttgcgg
    33481 ggcgagcggt gtgttggcgg gaacgtcagc agcgtagaga ggagccgcac ttaccaggct
    33541 gaccagccat aataaatttc gcgtatacat ggtattcctt accggagaaa ttataagccc
    33601 aacaacaata cgcctgtggc attgttcgct caaacttatc gcgagtgatt tggctcacac
    33661 tccggtaaag aaaaaagcga gttgcccccg cttttccatt aattaacgga tttcattcaa
    33721 tagttctgga tgcttatcca gaacacgtaa aagtttgatt gtggaaggat gaggttgggc
    33781 attgcctttt tcgtaacgcg aaaacgcatt tacacctccc ccaaaaattt cgcttgcctc
    33841 tttttgggta agagagagct tttttcgaac cttcactata aattcaggtg ccactgtttc
    33901 ggcattcacc gaagcccgaa atgcctttac ttgcgccatg aaagcatctg actcttcttt
    33961 attcatgatg ctctcttcgc aatggacaca atataaaccg tggatacctt tcaatactgt
    34021 ttttcgtcca cggaaggtgt atggaatatc tttaatgcca gaaaccattt ctccctggtg
    34081 gcaaaccgga catttcatat tacttctcct taaacgagac gatcagtacg tcatgaatta
    34141 ccgtaatttt aagataaacc tggcctgtaa caagcctggg tctgtaaaca tcctgccaga
    34201 tagtatgatc agagtaggtg gtcatgcttt tataaaagtc gctctctgat aatccaatga
    34261 taacattaca cataccatca aaatccaaac ctaactcatc tgcatttaac agggcactac
    34321 gtgttgtacg aacttgcccg gcattgacaa gttttttaac ctgactcaaa cgtgtatgtg
    34381 gtgtgcgttt ttccataacc cccgcctccc tgttacttta gttataacct aaaaggttaa
    34441 ttacagcaat gaaaaagcac ctaaaaggtt agttagatgt acggagatag tgaccacaca
    34501 aaacgtattc tttaaggaaa gtgattgacc atataagaaa gtggcgcatt agtagcgcca
    34561 gtttgaagca ggaatttata agggaagctg gagtcaggca tcacgccaac ggcacataaa
    34621 catccgtttc cagtcgctgc tctgtcaccc cttcggcaag attggtgtag tgaaacagaa
    34681 tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaatg atgccccata
    34741 ccgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa cgtccaccgg
    34801 taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta acgctgccgc
    34861 agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg gtcgtcccag gcgacgccaa
    34921 aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg ataaatttcg
    34981 ccacgctgta gttgagcaat tcagggctac agcggtgcgg caacatcgcc acaggtattg
    35041 atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct ccctgcgaaa
    35101 ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt gcggatctgt
    35161 ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat ctcaaccgta
    35221 tctttcggcg tcaccgtcac gccgttccag cgtttgtggt cgatatcaac attgatagta
    35281 ccgctggcat ctttgaacac gtagagatcg tcagagatgc gttcaacgat attgccgcgc
    35341 agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt tacgctgccg
    35401 ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg atgggccaga aaaaccgccc
    35461 tgctctgctg ccatcaccgg tgcgctgcac agggccatta ctgcgattac tgctgcgaat
    35521 tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa gatagtcctt
    35581 aacaacttct taagggaaaa aaataaaatt tagtgctgta cagagcgcgt tacaacacgg
    35641 tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt actgatagaa
    35701 gatgacatgc tgattggcga cggcatcaaa acgggcctta gtaaaatggg ttttagcgtc
    35761 gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc ttatgatgcg
    35821 gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg cgaatggcga
    35881 gaaaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc gcgatgcgct ggcggaacgt
    35941 gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc gttgatagaa
    36001 gtcgccgcca ggctggaagc tctgatgcgc cgaaccaacg gccaggccag caacgagctg
    36061 cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc tggcgaaccc
    36121 ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa cgctggtcgg
    36181 gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga ggtcaccagt
    36241 aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga ttttattcgt
    36301 accgtgcatg gtattggtta cacattaggt gagaaatgaa atttacccaa cgtcttagtc
    36361 tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg ctgctttcca
    36421 gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac acccaactga
    36481 tgctgtttgc caagcggtta agtacgctcg atctcaacga aatcaacgcg gcggatcgca
    36541 tggcacagac gccaaataga ttaaaacacg gtcatgttga tgacgatgcg ctgacctttg
    36601 ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga gaagatattc
    36661 cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa gacgatcctt
    36721 ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt ggccaggaat
    36781 gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc ccgtggctgg
    36841 tcgcactgcc gattatgtta atcatcatga tagtactact gggtcgtgaa ctcgcgccgc
    36901 tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa ccactaaacg
    36961 cgactggcgt acccagcgaa gtgcggccac tggttgagtc gctaaatcaa ctgttcgccc
    37021 gcacacatgc gatgatggtt cgtgaacgac gctttacctc cgacgcagct cacgaacttc
    37081 gtagcccgtt aacggcgctg aaagtgcaaa ccgaagttgc gcagctctct gacgatgatc
    37141 cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc gctactcgtc
    37201 tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt caggacgtcg
    37261 cggagatccc gcttgaagat ctcctgcaat cgtcggtgat ggatatttac cacacggcgc
    37321 agcaggcgaa aattgacgtg cgactgacac tcaatgccca cagcatcaaa cgcaccgggc
    37381 aaccgctatt gctaagtttg ttggtgcgaa atttgctgga taacgccgtg cgctacagtc
    37441 cacagggcag cgtggtagac gtcacgctga atgctgataa tttcatcgtg agggataacg
    37501 gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat cgcccacccg
    37561 gacaaaccgc taccggcagc gggcttgggc tatcgattgt ccagcgaatc gccaaattgc
    37621 atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg atttgaggcg aaggtaagct
    37681 ggtaacttct ctgtctatga gagccgttaa aacgactctc atagatttta ctaatagcaa
    37741 aatataaacc gtccccaaaa aagccaccaa ccacaaacca gacaaatacc gagcgagaat
    37801 acatataccc aaaactcgaa catttcccgc ataaagagtt ttcttaagat aagaataata
    37861 agtggcgtaa gaagaaaaaa tgctgcaatc attgcttgca atatacgatc ttcgcctgaa
    37921 acggaaggta tactgcaaat atttcctccc gactcaagca tccagtcata cttattga
//
LOCUS       DBBGLQ010000044        35164 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.044, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000044 DBBGLQ010000000
VERSION     DBBGLQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.044"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            404..2035
                     /locus_tag="ACIYSB_002848"
     CDS             404..2035
                     /locus_tag="ACIYSB_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429863.1"
                     /translation="MVTRAMQQKYDRVLSGESHPFPGIYEAVSEQLLLLIFKARQLYQ
                     NPEYRPWFEEQHRVLSALRANTKLLRIFREQRNADEPELIIKESTFPQLTNSDLTDAL
                     NIMCHLYNGFDEGNISLKTWGAMQEVQTTSTFISPRPGTWKTPELTKFVEDDLFNILT
                     ECKAWSSPIPAEARQGYCHQDLSVAHSCTSELLAHLNQYHEIDESAILARVISTTING
                     FYKKKNTDLTEWLSPFKEEHKIFRDCPEQNTGDVIRLKEQLLKTGRIEFVNNLCQVAN
                     WYEIKSIYDEYVRLYANEIISWFNKTMAYFKPTDFIRYTWKFATVTPDKDTETGHTTA
                     WEKDYCQITIQRDELLRLIHTNTLQDAVIYLCHCLQEKYKKSSQNETEFPGIKPPASE
                     SEINITDNIEFYSLLELSKDNEKRLETFQKWYEKNKVLLESHKQKRTEKIDVAVRLVG
                     LKAYDLHEGIPDRPKRKVKDGINEDIKADTSLRLSKTNISDTSLNRYRRTVKEIINNE
                     IDVFLLEQKKKNKRFPYSEDRDVIRPLWGKCLTGQ"
     gene            complement(2122..2703)
                     /locus_tag="ACIYSB_002849"
     CDS             complement(2122..2703)
                     /locus_tag="ACIYSB_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429864.1"
                     /translation="MSYSPATVARRFSILDGVTIQGVLYQIIWDPETPFAAVIEAAPS
                     VIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVGLATFVY
                     ETIATKAGVILLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGDRICYD
                     GESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            complement(3029..3104)
                     /locus_tag="ACIYSB_002850"
     tRNA            complement(3029..3104)
                     /locus_tag="ACIYSB_002850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3069..3071),aa:Thr,seq:cgt)
     gene            complement(3219..4472)
                     /gene="proA"
                     /locus_tag="ACIYSB_002851"
     CDS             complement(3219..4472)
                     /gene="proA"
                     /locus_tag="ACIYSB_002851"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGC5429865.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(4484..5587)
                     /gene="proB"
                     /locus_tag="ACIYSB_002852"
     CDS             complement(4484..5587)
                     /gene="proB"
                     /locus_tag="ACIYSB_002852"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGC5429866.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            5875..6930
                     /gene="phoE"
                     /locus_tag="ACIYSB_002853"
     CDS             5875..6930
                     /gene="phoE"
                     /locus_tag="ACIYSB_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGC5429867.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(6969..7370)
                     /gene="crl"
                     /locus_tag="ACIYSB_002854"
     CDS             complement(6969..7370)
                     /gene="crl"
                     /locus_tag="ACIYSB_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGC5429868.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(7428..8672)
                     /gene="frsA"
                     /locus_tag="ACIYSB_002855"
     CDS             complement(7428..8672)
                     /gene="frsA"
                     /locus_tag="ACIYSB_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGC5429869.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEDDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(8764..9222)
                     /gene="gpt"
                     /locus_tag="ACIYSB_002856"
     CDS             complement(8764..9222)
                     /gene="gpt"
                     /locus_tag="ACIYSB_002856"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGC5429870.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            9483..10940
                     /gene="pepD"
                     /locus_tag="ACIYSB_002857"
     CDS             9483..10940
                     /gene="pepD"
                     /locus_tag="ACIYSB_002857"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGC5429871.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQGNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(10997..11518)
                     /gene="prfH"
                     /locus_tag="ACIYSB_002858"
                     /pseudo
     CDS             complement(10997..11518)
                     /gene="prfH"
                     /locus_tag="ACIYSB_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(11514..11720)
                     /locus_tag="ACIYSB_002859"
                     /pseudo
     CDS             complement(11514..11720)
                     /locus_tag="ACIYSB_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(12038..12490)
                     /gene="yafP"
                     /locus_tag="ACIYSB_002860"
     CDS             complement(12038..12490)
                     /gene="yafP"
                     /locus_tag="ACIYSB_002860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGC5429872.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQISAWAQI
                     DESRWKEKLAKSQVWVAIINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(12500..12898)
                     /gene="yafO"
                     /locus_tag="ACIYSB_002861"
     CDS             complement(12500..12898)
                     /gene="yafO"
                     /locus_tag="ACIYSB_002861"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HGC5429873.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(12901..13194)
                     /gene="yafN"
                     /locus_tag="ACIYSB_002862"
     CDS             complement(12901..13194)
                     /gene="yafN"
                     /locus_tag="ACIYSB_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HGC5429874.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALIDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(13246..14301)
                     /gene="dinB"
                     /locus_tag="ACIYSB_002863"
     CDS             complement(13246..14301)
                     /gene="dinB"
                     /locus_tag="ACIYSB_002863"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGC5429875.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(14372..15157)
                     /gene="lafU"
                     /locus_tag="ACIYSB_002864"
     CDS             complement(14372..15157)
                     /gene="lafU"
                     /locus_tag="ACIYSB_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGC5429876.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            15129..16754
                     /locus_tag="ACIYSB_002865"
                     /pseudo
     CDS             15129..16754
                     /locus_tag="ACIYSB_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(16978..17475)
                     /gene="rayT"
                     /locus_tag="ACIYSB_002866"
     CDS             complement(16978..17475)
                     /gene="rayT"
                     /locus_tag="ACIYSB_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HGC5429877.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRHAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDFSAG
                     ERIIS"
     gene            complement(17651..18400)
                     /gene="yafL"
                     /locus_tag="ACIYSB_002867"
     CDS             complement(17651..18400)
                     /gene="yafL"
                     /locus_tag="ACIYSB_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGC5429878.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESKRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRISRLAEPFWQDHFLGARRILTEETIL"
     gene            18610..18870
                     /gene="dinJ"
                     /locus_tag="ACIYSB_002868"
     CDS             18610..18870
                     /gene="dinJ"
                     /locus_tag="ACIYSB_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGC5429879.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDLFDKLGI"
     gene            18873..19151
                     /gene="yafQ"
                     /locus_tag="ACIYSB_002869"
     CDS             18873..19151
                     /gene="yafQ"
                     /locus_tag="ACIYSB_002869"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGC5429880.1"
                     /translation="MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            19307..20047
                     /gene="dpaA"
                     /locus_tag="ACIYSB_002870"
     CDS             19307..20047
                     /gene="dpaA"
                     /locus_tag="ACIYSB_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGC5429881.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(20018..20785)
                     /gene="yafJ"
                     /locus_tag="ACIYSB_002871"
     CDS             complement(20018..20785)
                     /gene="yafJ"
                     /locus_tag="ACIYSB_002871"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGC5429882.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(20991..21569)
                     /gene="lpcA"
                     /locus_tag="ACIYSB_002872"
     CDS             complement(20991..21569)
                     /gene="lpcA"
                     /locus_tag="ACIYSB_002872"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGC5429883.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            21809..24253
                     /gene="fadE"
                     /locus_tag="ACIYSB_002873"
     CDS             21809..24253
                     /gene="fadE"
                     /locus_tag="ACIYSB_002873"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGC5429884.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALVKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(24296..24742)
                     /gene="ivy"
                     /locus_tag="ACIYSB_002874"
     CDS             complement(24296..24742)
                     /gene="ivy"
                     /locus_tag="ACIYSB_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGC5429885.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            24923..25693
                     /gene="yafV"
                     /locus_tag="ACIYSB_002875"
     CDS             24923..25693
                     /gene="yafV"
                     /locus_tag="ACIYSB_002875"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGC5429886.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAALREYREKFPAWQDADEFRLW"
     gene            complement(25734..26870)
                     /locus_tag="ACIYSB_002876"
     CDS             complement(25734..26870)
                     /locus_tag="ACIYSB_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGC5429887.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPSKFHECFINWMRDCHTSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHRRLDVVMNEDDYKIRRGNAAELFS
                     GIRHIAINILTNEKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            complement(27301..27693)
                     /locus_tag="ACIYSB_002877"
     CDS             complement(27301..27693)
                     /locus_tag="ACIYSB_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429888.1"
                     /translation="MNVLRHVNAINMGVCILIWVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIKEGGAVSFIFNEETLYFTVFGGDFIMDDLKKVKNT
                     EAWVWYKNCDSKNKNVAVELLQIYELHQ"
     gene            complement(27671..31915)
                     /locus_tag="ACIYSB_002878"
     CDS             complement(27671..31915)
                     /locus_tag="ACIYSB_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HGC5429889.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPTKK
                     DSPNYGSPVNSLLGAKVLPVETDLALPGPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQVHERELILNDSGGRSIHFESLFPGEISYSRSESFWLARGGVLKQHKGHPLA
                     RLWRALPEAVRLSPHTYMMAVSTTGQWLILGWPERVPEADEVPPPEPPAYRVLTGVVD
                     GFGRTLTFHRAAEGDVAGAVTGVTDGAGRCFHLVLSTQAQRAEAFRKQRESSLSSPAG
                     PRSASSSQFFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGE
                     LRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYR
                     FEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDA
                     AGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQLQLTSVTYPDGLRSSRKYDRQGRL
                     AEETSRNGNITRWFYDFSRSGLPCAVEDGTGVRRRITRNRYGQLLAFTDCSGYTTRYE
                     YDQYGQQIAVHREEGISTYSSYNPRGQLISRKDAQGRETRYEYSAAGDLTATISPDGK
                     RSATEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRG
                     FDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLT
                     TISHTSEGHRVAVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQE
                     PDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAY
                     TLTGQLRSRHLNLPQLDREYTWNDNGQLVRISGPQESREYRYSDTGRLTGVHTTAANL
                     DIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDRIP
                     EGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERD
                     LTGWMSLSRKPEVTWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAKA
                     RHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQ
                     MEDAYIPERRLHLYHCDHRGLPQALITPEGETAWCGEYDEWGNQLNKENPHHLYQPYR
                     LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLVPIRYTDPLGLE
                     RVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGAG
                     DPYTGEVVGCETNESGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTMG
                     CTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            complement(31991..34132)
                     /locus_tag="ACIYSB_002879"
     CDS             complement(31991..34132)
                     /locus_tag="ACIYSB_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGC5429890.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(34342..34860)
                     /locus_tag="ACIYSB_002880"
     CDS             complement(34342..34860)
                     /locus_tag="ACIYSB_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGC5429891.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
BASE COUNT         8476 a         9290 c         8652 g         8746 t
ORIGIN      
        1 ggcgtagata tataaattaa tccatgtaat gttcaggcaa ggcatggagg agtcgcagga
       61 aggtgaaata tttcaatcta atttgtgata gtcatcacaa aaataagcta taacaagtgg
      121 aggaaaagac aatctgttgc tgaacagaca gaatcaggct acgaataatc agaaaataaa
      181 gcaaatactc ttatacagaa gaagcagggg taactgacgg agacaatcac aatcaggaga
      241 aaattatcgt aaataatgta taaaatctca ggatataaac aaatgtacac aagctatcca
      301 cgtaatcagc ggctgtctca ggattaccgt ttcatttcag tggacgacat cgattctgtc
      361 tgacagtggg ccaatatgtg gcaagcaaaa aacttctgac atcatggtaa ctcgggcaat
      421 gcaacaaaag tatgatcgtg ttttatccgg cgaatcccac ccattccctg gcatatacga
      481 ggcggtttcc gaacaactcc ttttactcat ttttaaggct cgtcagctat atcagaatcc
      541 agagtatcgc ccctggtttg aagaacagca cagagttctc tctgcattac gtgctaatac
      601 aaagctcttg agaatcttta gagaacaaag aaacgcggat gaaccagagc ttattattaa
      661 agaatctaca tttccccaac tcactaattc tgatcttaca gatgcgctta atattatgtg
      721 ccatttatac aatggttttg atgaaggaaa tatcagcctg aaaacctggg gggcgatgca
      781 agaagtacag acaacatcaa cctttatcag cccgagaccg ggaacctgga aaacacctga
      841 actgacaaag tttgtagaag atgatttatt taatattctg actgaatgta aagcctggag
      901 ttcaccaatt ccagcagagg cccgacaggg gtattgccac caggatctct ctgttgcaca
      961 ctcctgcacc tcagagttac tggcacacct gaatcaatac catgaaattg atgaatcagc
     1021 aatacttgcc agagttatta gtacgacgat taacggattc tacaagaaaa aaaatacaga
     1081 ccttacggaa tggctatccc cttttaaaga ggaacataaa atattcaggg attgtccgga
     1141 gcaaaatacc ggggatgtaa tcagactgaa agagcaactg ttgaaaacag gacgtataga
     1201 attcgtgaat aatttgtgtc aagtcgccaa ctggtatgaa attaagtcaa tatacgatga
     1261 atatgtccgt ctttatgcca atgaaatcat tagttggttc aacaaaacca tggcgtattt
     1321 taaaccaaca gattttatcc gatatacatg gaaattcgca acagttactc cggataaaga
     1381 tacagaaacc gggcatacaa ctgcatggga aaaggattat tgccagataa ccatacaacg
     1441 tgatgaactt ctgaggttga ttcacactaa tacccttcag gatgcagtaa tttatctttg
     1501 tcattgtctg caggagaaat acaagaaatc atcccagaat gagacagagt ttccaggaat
     1561 aaaacctccg gcatcagaga gtgaaattaa tataacagat aatattgaat tttattctct
     1621 tctggagctc agtaaagata atgagaagcg gctggaaaca tttcaaaaat ggtatgaaaa
     1681 aaataaagtt ttgttagaaa gtcataaaca aaaacgaaca gagaagattg acgttgccgt
     1741 acgacttgta gggctgaaag cctatgatct gcatgaaggt attccggata ggccaaaacg
     1801 aaaagttaaa gatggcataa atgaagacat caaagccgat accagtttac gcctgtcaaa
     1861 gacaaatata agtgatacgt cgctaaaccg ttatcgccgc actgttaagg agattatcaa
     1921 taacgaaatc gacgtattcc tgttggaaca gaagaaaaaa aataaaagat ttccctacag
     1981 tgaagataga gatgtgattc gacctttatg gggcaaatgc ctgaccggac agtaactccc
     2041 tgaggggata acagcccggt tatcccctgt tcgttatgcc tcggtatacc ggagttccag
     2101 ggacacgaga caatcggcaa attatgcggc ttttccatta acagattcgg ccacaagaac
     2161 aacaccgtgt ttgtttcgat caggatgttc actccagatt tctgattcag gaatactttc
     2221 cccatcatag cagatccgat caccatcaaa cggataatac cgtgcggaat ctgtatctaa
     2281 gataaacacc ttaagattcg ttgaacgacg cgctatatgc tgccagaggg ctttacctcc
     2341 ttcatattgc tcgttatcac ttaatagtat aacgcctgct tttgtggcta ttgtttcata
     2401 aacaaacgta gcaaggccaa catcacgtac agcgccatcg acaacaatcc cttcaatctg
     2461 agcaacgtcc tgttcttccc agaatttccg gacaaacacc ccttcaagct gtgaacgacg
     2521 ctgaagctcc agagtggcaa caaccttatg gcgaatatca ccatcaataa cagaaggtgc
     2581 agcttcgatt accgcagcga acggagtctc tggatcccag atgatctggt aaagcacgcc
     2641 ctgaatagtt actccatcca gtatcgaaaa acggcgggct acagtagcag gagagtaaga
     2701 cattggaatg acagctattt tatggcctaa aatttgaaaa cattagtatc aatactgacc
     2761 tcatcagtct gagcatatat tgataaagtt tttatgttta cactcaataa ataccttgcc
     2821 aaacaactaa tagcgatgtc tacattgcca ttcgacatgc ttacaatttt tgtcaacaag
     2881 aaagtgaatt tatatcaacg gtaagaagta aacacgagga caccaaaacc aaatgtacac
     2941 acttttgtgc tttgtgtccc ctgtgtgtac ccaaatgaca agctgcaaga aaatctaggc
     3001 acacacttat ctaacttatt gatttaaatg gtgccgataa taggagtcga acctacgacc
     3061 ttcgcattac gaatgcgctg ctctaccaac tgagctatat cggccctgaa aggacatgtt
     3121 cacgaacgtg aatcacggtg gacaaggtta aaactaaccg ggcgatgcgt caatggcctt
     3181 gtgaatcaaa tggctacttt tgcatcaccc ggttttattt acgcacgaat ggtgtaatca
     3241 ccaatgccga tccacttgta agtggtcagt gcttccagcc ccattgggcc acgcgcgtgg
     3301 agtttttgtg tgcttaccgc cacttccgca cccagaccaa actggccgcc gtcggtaaaa
     3361 cgcgtagagg cgttaacgta aacagcggac gaatccactt cgttaacaaa acgctgggcg
     3421 ttgcgcatat cgcgggtcag gatcgcatcg gagtgttgtg tgccgtgttc acgaatatgg
     3481 gcgatggcat cgtcaagatc gctgacgatt ttgacgttca aatctaatga cagaaactca
     3541 tcgtcatact cttcggcttt aacagcaacc accttcgcag ggcctgcctg caactgcgcc
     3601 agtgcagctg catctgcgtg taatgtcacg ccgctttccg ccatttgttt gcttaatgcg
     3661 ggcaggaagc tatcggcgat gtttttattc accagcaacg tttcaaccgt attacatgtg
     3721 ctcggacgct gagttttcgc gttgacgatc acttttaatg cttcagcgat ctctacactt
     3781 tcatcaacgt aaatatggca tacgcctata ccacctgtga tcaccgggat tgtcgactgt
     3841 tcacggcaca gtttatgcaa accagcgcca ccacgcggga tcagcatgtc gatgtattta
     3901 tccatacgca gcatttcact gaccagcgca cggtcaggat tatcaatcgc ctgcacggca
     3961 cccgccggta agccgcagga tttcagggcg tcctgaatca ccgccaccgt tgcagcgtta
     4021 gtgcgacacg tttctttgcc accgcgcagg atcaccgcat taccggtttt caggcacagc
     4081 gaagcgacat caaccgtcac gttcgggcgc gcttcataaa tcacgccaat aacccccagc
     4141 ggtacgcgac gacgctcaag acgcaggccg ctgtccagta cgccgccatc gattacctgc
     4201 cccaccggat cggcgaggtt gcacacctga cgtacatcgt cggcaatgcc tttcagccgt
     4261 gcgggcgtca gtgccagacg gtcaagcatc gcttcgctaa ggccattggc tcgcgcgtca
     4321 gcaacatcct gggcgttagc gttgaggatg atttcgcttt gtgcttccag ttcatcggcg
     4381 attttttcca gcacgcgatt tttttcgcgg ctggagagtt gcgctaattt atacgaggct
     4441 tgcttcgcgg caatgcccat ttgttccagc atcagcctgc tccttaacgg gtaatcatgt
     4501 catcacggtg aacggcaacc gggccgtatt catatcccag tattgcatca atttcttgcg
     4561 agtggtgtcc ggcaatacgg cgtaatgcat cgctgttgta acgactgacg ccgtgggcga
     4621 tatcgcggcc ttcgaggttg caaatgcgga tgacttcacc acgcgagaaa ttgccagtca
     4681 cgcttttaat gcctttcggc aacagggagc tgccgcgttc cagaatggcg gcagttgccc
     4741 cttcatctac cgtgatttca cccgccggcg gcgcaccgaa aatccagcgt ttacggtttt
     4801 caagcggagt cgcctgggca tggaacagcg taccgacgga aatgccttcc atcacatcac
     4861 caataacgcc cggcttgctg cccgcggcaa taatggtgtc gatacccgca cggcaagcca
     4921 cgtcagcggc ctgcaatttg gtactcatgc cgccagttcc gaggcctgaa acgctgtcac
     4981 cggcaatcgc gcgcagtgcg tcatcaatgc cgtaaacatc tttaatcagt tctgcctgcg
     5041 gattgctgcg cgggtcagcg gtatacaaac ctttttgatc ggtcagcagc aacagtttat
     5101 cggcacccgc aagaatcgcc gccagcgcag aaaggttatc gttatcgccg accttaatct
     5161 ctgccgtagc gacagcatcg ttctcattga ttaccggaac gatattgtta tcgagcaacg
     5221 ctcgcagggt gtcgcgggcg ttcaggaagc gttcacggtc ttccatatca gcacgggtca
     5281 gcagcatttg cccgacgtga atgccataaa tcgaaaacag ctgttcccac agttgaatca
     5341 gtcgactctg ccctaccgcc gccagcagtt gtttcgaggc gatggtcgct ggcagttccg
     5401 ggtaacccag gtgctcacgt ccggcggcga tcgcgcccga cgtcacaata acaatccgat
     5461 gcccggcggc atgtaactgc gcgcactggc gaacaagttc aacgatatgg gcacggttca
     5521 gacggcgcga tccgcctgtt agcacactgg tgccgagttt taccaccagc gtctggctgt
     5581 cactcatgat tctctgccat tcaattttag gaaaaatgat atcaaacgaa cgttttagca
     5641 ggactgtcgt cggttgccaa ccatctgcga gcaaagcatg gcgttttgtt gcgcgggatc
     5701 agcaagccta gcggcagttg tttacgcttt tattacagat ttaataaatt accacatttt
     5761 aagaatatta ttaatctgta atatatcttt aacaatctca ggttaaaaac tttcctgttt
     5821 tcaacgggac tctcccgctg aatattcgcg cgttaattaa aatcaggaat gaaaatgaaa
     5881 aagagcactc tggcattagt ggtgatgggc attgtggcat ctgcatctgt acaggctgca
     5941 gaaatatata ataaagacgg taataaactg gatgtctatg gcaaagttaa agccatgcat
     6001 tatatgagtg ataacgccag taaagatggc gaccagagtt atatccgttt tggttttaaa
     6061 ggcgaaacac aaattaacga tcaactgact ggttatggtc gttgggaagc agagtttgcc
     6121 ggtaataaag cagagagtga tactgcacag caaaaaacgc gtctcgcttt tgccgggttg
     6181 aaatataaag atttgggttc tttcgattat ggtcgtaacc tgggggcgtt gtatgacgtg
     6241 gaagcctgga ccgatatgtt cccggaattt ggtggcgatt cctcggcgca gaccgacaac
     6301 tttatgacca aacgcgccag cggtctggcg acgtatcgga acaccgactt cttcggcgtt
     6361 atcgatggcc tgaacttaac cctgcaatat caagggaaaa acgaaaaccg cgacgttaaa
     6421 aagcaaaacg gcgatggctt cggcacgtca ttgacatatg actttggcgg cagcgatttc
     6481 gccattagtg gggcctatac caactcagat cgcaccaacg agcagaacct gcaaagccgt
     6541 ggcacaggca agcgtgcaga agcatgggca acaggtctga aatacgatgc caataatatt
     6601 tatctggcaa ctttctattc tgaaacacgc aaaatgacgc caataactgg cggctttgcc
     6661 aataagacac agaactttga agcggtcgct caataccagt ttgactttgg tctgcgtcca
     6721 tcgctgggtt atgtcttatc gaaagggaaa gatattgaag gtatcggtga tgaagatctg
     6781 gtcaattata tcgacgtcgg tgctacgtat tatttcaaca aaaatatgtc agcgtttgtt
     6841 gattataaaa tcaaccaact ggatagcgat aacaaattga atattaataa tgatgatatt
     6901 gtcgcggttg gcatgacgta tcagttttaa tgaatattgc cggatgtgat gcatccggca
     6961 catttcactc acgccgttaa cttcaccggc tcgtcacgaa aatcatccgc cggttccagc
     7021 ttcagattca gcgtcgtcag cagttcacgc agcttctcgt gaaactcacg cagggtgtgc
     7081 tccagtcgtt caaccacttc agtgtctttt accggaacac tcttccagtc gcctgcttta
     7141 tcgaacagac caaactggta actgtaggta aaacgggatt cctgcgcttc cagctccatc
     7201 caccagcccc agaattcacg cacttccggt gccggtttca cgttgacgca tacagccaga
     7261 caatcgaaaa agaatcgatt atcttcgcac ttaccttcac gaatatacgg gcctagtgcg
     7321 gtaaattttt tgatcaatct gctcttcggg tgtccactcg gtaacgtcat tgctatctcc
     7381 tgttgtgatg caactgtttt accaaattgg caaaatttag caaattttta acacaagcgt
     7441 ttttcgatcc agtcggtgat ttcctgaaga cctttgtcaa aattccgata caccgggtta
     7501 aatgggatct ctaataattt accgtcagca gatgatgagg tgattaagcg tgagtcctct
     7561 tccgggctga acggatcgtt cttccagtag cctgataaca ttggcgttgg gcagcgacgt
     7621 cccagcaatc cttgcacttt taatgaatag cgattcagct ccacgcgcaa cgcttcatcg
     7681 gaagcatcat gcatccccaa acgactggcc agaacgtcaa gatacatttc cggcacctgt
     7741 tgctggcact taaaatcact caacagggta tgaactaccg gaccaagaca ggcaaccgct
     7801 ttcagacgcg gcgattcaag gtatgccaga cgcacggcaa cgttagcgcc gaaacggaaa
     7861 ccaaaggccg cgacgcgagt gtgatccacc cacggtacgt taggcagcgc ctttaagacg
     7921 tgctgatgca acaggctgga gtcctgggtg agcttccatt ttgaagaaaa gcccaccgac
     7981 ggcatatcaa tagtcagcat cgcaatgccg cgcggcgcaa aataacgttc atacaggctg
     8041 taatagtccg tctgcatcgc atccagacca ccacacatta ataccgtcgg gaacgggcca
     8101 tcgcctttcg gcatatgcaa aaagccggtg atgggcgcac cgccgggtac ggtaaactcc
     8161 atctgccgca tcgtgcccgg tagacgctga gcggcctctt cataggcgcg gtttgacaaa
     8221 gcctgcgctt gctcggccag gtcatctcct ttcagatgag gataggcggc aatgttgtac
     8281 aacgtagccg catgcagcca gtgacgacca ctgagttgcg gatcgtcttc cgcacaggct
     8341 ttttgttgcc acaccatcgc ctgggtggcc cactcataaa tccagttgcc gccacggtag
     8401 ccaatcaccg tatcgtataa atcatcgtcg gtacgttcgg catcgctcat cacaatacgt
     8461 gcctggacgt cgaggatttc gcgtgggtca atgccgcgcc agatccacat cagacggtta
     8521 atcatgcgat accagtgagg gatggtttta ccatcaaggg ccgactgcac aggcggttgt
     8581 gcgccgtgat taaagcggcg gactagcgtc gaggtttcag gatgtttaaa gcggggtttg
     8641 aacagggttt cgctcaggtt tgcctgtgtc atggatgcag cctccagaat acttactgga
     8701 aactattgta acccgcctga agttaaaaag aacaacgccc ggcagtgcca ggcgttgaaa
     8761 agattagcga ccggagattg gcgggacgaa tacgacgccc atatcccacg gctgttcaat
     8821 ccaggtatct tgcgggatat caacaacata gtcatcaacc agcggacgac cagccggttt
     8881 tgcgaagatg gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa ccgcagtacc
     8941 accggtatcc accaggtcat caataacgat gaagccttcg ccatcgcctt ctgcgcgttt
     9001 cagcacttta agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa cggtatcgac
     9061 atgacgaata cccagttcac gcgccagtaa cgcacccggt accagaccgc cacggcttac
     9121 ggcaataatg cctttccatt gttcagaagg catcagtcgg cttgcgagtt tacgtgcatg
     9181 gatctgcaac atgtcccagg tgacgatgta tttttcgctc atgtgaagtg tcccagcctg
     9241 tttatctacg gcttaaaaag tgttcgaggg gaaaataggt tgcgcgagat tatagagatc
     9301 tggcgcacta aaaaccagta tttcacatga gtccgcgtct ttttacgcac tgcctctccc
     9361 tgacgcggga taaagtggta ttctcaaaca tatctcgcaa gcctgtcttg tgttgacaac
     9421 attttctgct aaccctgtga cctgcaatac tgttttgcgg gtgatcgaca aggagactta
     9481 acgtgtctga actgtctcaa ttatctccac agccgctgtg ggatattttt gccaaaatct
     9541 gttctattcc tcacccgtcc tatcatgaag agcaactcgc tgaatacatt gttggttggg
     9601 caaaagagaa aggtttccat gtcgaacgcg atcaggtagg taatatcctg attcgtaaac
     9661 ctgctaccgc aggtatggaa aatcgtaaac cggtcgtctt acaggcccac ctcgatatgg
     9721 tgccgcagaa aaataacgac accgtgcatg acttcacgaa agatcctatc cagccttata
     9781 ttgatggcga atgggttaaa gcgcgcggca ccacgctggg tgcggataac ggcattggta
     9841 tggcctctgc gctggcggtt ctggctgacg aaaacgtggt tcacggcccg ctggaagtgc
     9901 tgctgaccat gaccgaagaa gccggtatgg acggtgcgtt cggcttacag ggcaactggt
     9961 tgcaggctga tattctgatt aacaccgact ccgaagaaga aggtgaaatc tacatgggtt
    10021 gtgcgggggg tatcgacttc acctccaacc tgcatttaga tcgtgaagcg gttccagctg
    10081 gttttgaaac cttcaagtta accttaaaag gtctgaaagg cggtcactcc ggcggggaaa
    10141 tccacgttgg gctgggtaat gccaacaaac tgctggtgcg cttcctggcg ggtcatgcgg
    10201 aagaactgga tctgcgcctt atcgatttca acggcggcac actgcgtaac gccatcccgc
    10261 gtgaagcctt tgcgaccatt gctgtcgcag ctgataaagt cgacgtcctg aaatctctgg
    10321 tgaataccta tcaggagatc ctgaaaaacg agctggcaga aaaagagaaa aatctggcct
    10381 tgttgctgga ctctgtagcg aacgataaag ctgccctgat tgcgaaatct cgcgatacct
    10441 ttattcgtct gctgaacgcc accccgaacg gtgtgattcg taactccgat gtagccaaag
    10501 gtgtggttga aacctccctg aacgtcggtg tggtgaccat gactgacaat aacgtagaaa
    10561 ttcactgcct gatccgttca ctgatcgaca gcggtaaaga ctacgtggtg agcatgctgg
    10621 attcgctggg taaactggct ggcgcgaaaa ccgaagcgaa aggcgcatat cctggctggc
    10681 agccggacgc taattctccg gtgatgcatc tggtacgtga aacctatcag cgcctgttca
    10741 acaagacgcc gaacatccag attatccacg cgggcctgga atgtggtctg ttcaaaaaac
    10801 cgtatccgga aatggacatg gtttctatcg ggccaactat caccggtcca cactctccgg
    10861 atgagcaagt tcacatcgaa agcgtaggtc attactggac actgctgact gaactgctga
    10921 aagaaattcc ggcgaagtaa ttatttgatt tgctgccgga tggcgtttaa tcgccttccg
    10981 gcagtttcat ccttcattat ccttcgataa aagccatccc tgtaaatgtc cgtcgcgggt
    11041 tgccacgttc aatctggtga tggaacattc gccgctgcga tttcagcgcc gcgctatttt
    11101 cctgttgctg ttgctccagc ttccaggcaa tcagcaatcg tgccagccgc ttgttagcat
    11161 gctgactacg ctctgactga accttcacgc taataccgga tgccaaatgc gtggcgcgta
    11221 ccgccgagtc ggttttattg acatgttgac cgcccggccc cgacgaacgc agcgtctcat
    11281 aacggattgc atccgattgt tcctgctcat cagcggtaaa acgcccaatg cccagaaacc
    11341 agtttttgcg cccatgatga ggccgatacg gactcggaca aatccactga atagtgccac
    11401 accagctttc gcttaatgcc catgcgttat cgccatcgag agaaaccagc gccgaacgca
    11461 gtgtgtcaga gtagcggccc gtttctgttt ccagcaccgt taccgcgacg tcttgccggg
    11521 taaaaacgac caacagagaa gaacgctgcg ccaatcgggt agccgcgtcc ggggtgtaaa
    11581 tctggcatcc ccactacgcg ctgcatgttg ggtaaatttg ccgtggtgtg taattgttgg
    11641 atcgctaaac tctcgatcca caggtcatca gatgcgatgg taaataccgc atcggataag
    11701 ggacgaatat atttgcccat gtaccattcc atttatactc gtcatacttc aagttgcatg
    11761 tactgcgact gcgttcgttc accccagtca cttacttatg taagctcctg gcgattcact
    11821 cgcttgtcgc cttcctgcaa ctcgaattat ttagagtata taaatataaa aatcaggaaa
    11881 tggcagacga atccagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
    11941 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaatcag aaaacgcgca
    12001 cataccaggc gggcgttatt ttcattgcaa gctggattta atgttgcggt ttatatcgca
    12061 tataaaaatt agtaaaccac gctccccggc attcaacgcg ctgctgctta actgtctgaa
    12121 aaccataacg ttcaaaaaag ggttttgcgg ttatgcttgc gtccaccgta agttcggatt
    12181 cagacttaat caaaggtttt aacaaagcgc tggcaacccc acggcgggtg tattcagggt
    12241 caacaaataa catatcgata taatgttcaa tgcgggaaat aaaaccaacc ggttgtgcat
    12301 taatgatcgc aacccacact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
    12361 tctgcgccca ggcggaaatt tgttgtggtg aataatgctg actggcggtc atcgtaaccg
    12421 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagttt cttatttgta
    12481 tgttattcat aatataaatt caaaaacgca tgcgaaacgc ttctgccatt ttcccaattt
    12541 tatgcatttg gttgttatct cgagccagtt tatgaggttc aggtttcaga atggcaatga
    12601 gcaaccatgc ttgctcatca aacgccccct gacaatatac caaatgcgct tcgtcattcg
    12661 ttctgctgaa ttggcgcaac tgtggcggaa atggattatt ctcatttgcc agatgaatat
    12721 gagcaactcg ctcaaatttg attaatggcc aggtaaagga gtcgtcgtag agtgcatcgc
    12781 gaccaaatat atctggcaaa acaccgtcac gcttatagga aataaaatcc gccgttaacg
    12841 catcaagttc ctctgctgta agttgcaggc gaataagttt tgttttgaat acccgcatcc
    12901 ttattcctta aagtcattga aatcatcatc cgtcatatca ttaagatatt gttcagcgcg
    12961 gcgtgtaatt tcctctaatc ttgcagcact tggacggaag cgcgccttta tgggcttttt
    13021 ctcctcttgt tcagcaagca tgtctattaa cgcttcgaat gcgctggcac ttaagagata
    13081 tcctgcgggg cgattattag aaagaaccgc aaccggttga tcaataaagt atttagctgg
    13141 gtttttacgt aactcagtga tattgaccga tttttcagcg agaattcgat gcatacagtg
    13201 ataccctcat aataatgcac accagaatat acataatagt atacatcata atcccagcac
    13261 cagttgtctt tccatttgcg ggtcaagcaa cgtcacatgc agccccacca gacgcacacc
    13321 gcgcccgccg cggcgttcat cccaggtttt acgcgcggtg gcgattagat cagctttatt
    13381 cagccgcggc cagacgtgct cctgggtggt ttgctgaaaa tcgtcgaact ttaatttcac
    13441 cccctggcga gcaatcagta aatcaggttt tacctttgcc agacggcgtt caagttccgg
    13501 atacagccgc tcgataatcg cttcacattc agaccagtga tgaatatctt ccgccatcgt
    13561 gcgttccacg ccgacggatt ttcgcaaccg ttcgctgtta acatcgcgtt cgtcaatccc
    13621 ctgactacgc tcccacaaaa tgcggccaaa tttgccaaag cgtttaagca gcatcaccag
    13681 atcacacttt tgtacatcac cgcaggtccg cagccccatc gcttccagtt ttgccgctga
    13741 gactttgccg acgccgggga tttttgccag cggtaaggtt tgtaaaaatg ccggaacttc
    13801 tgccggcgta atcacaaact ggccgttggg tttattcatg tcggaggcga ttttggcgag
    13861 aaactttact ggtgccacgc ccgcagacgc cgtcagttgc agctcgttga agattgtctg
    13921 gcggatttcc tgggcgatga gggtcgcaga accgtggcaa tggacgctat cggtgacatc
    13981 gagataagcc tcatccagtg acaacggttc aatgcgcgag gtgtagcgcg agaagatttc
    14041 acggatatga tttgaggctt ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg
    14101 tgggcataat ttgagcgcca tccctgtcgg catagcgcta cgtacgccaa atttacgcgc
    14161 gggataattg gcggtgctga tcaccccccg acgttcgcgg ctgccgccaa tagcaatagg
    14221 gatatcgcgc agggcgggat tgtcgcgcat ctccactgcg gcgaaaaagc agtccatatc
    14281 cacatgaatg attttacgca ttgctcacct ctcaacactg gtaaagtata cagtgatttc
    14341 agggtttgag aaatgcgtaa agattcagca tttaacgcgt tcgctgcgaa agcacctgct
    14401 gcttatccag cttttgcacc agcggctgca ccactttatc cccatgctga ccaaagtatt
    14461 gatacagcgt atcggacgca cttttggtca gcaccataat ctcaatgcgc cggttgcccg
    14521 cgctttgcgg atttttggaa tccagcagca tctggtccgc cattgcgctt acctgcatca
    14581 ctttatcttc cggcattccg gcctcttcca gcacccgacg agccgaaagc gcgcggtcac
    14641 ccgaaaggtt ccagttgttg tagatattgt ttttgtaggc catcgcatcg gtatgcccgg
    14701 taataataat tttattatcg agcgagtcga acactggcgc gagctccacc agcagcgttt
    14761 taaagaacgg cataatcttc gcgctgccgc gttcaaacat attgcggttc tggtcgtctt
    14821 taatcagcac gcgtaatccc tggggaacaa tctccatctc cagattcgct tccatatgcg
    14881 catcacgggc aatggtgttg atactggttg ccagctcacc aagctcggtg gcggatttct
    14941 tcttcagcag cgccgttttc tcattcacat cacgcgcttt tttctccgtc tcttccggca
    15001 ccgccacggt ggcaggtttt ggcggatgag acgggctgat tttatttaac ggcgacagcc
    15061 caccaccgtt aaaaatcgac tgcccgtgca gcgcggcgat aatgctttca cgctcggatt
    15121 tactgacgct gttgacaatc aactttattg tcgtcaccaa aggggccgag cgtatttccg
    15181 aggtttctgc ccgctttacc ttagacgcga tgcccggcaa acagatggcg attgacgccg
    15241 atcttaacgc cggattgatc aaccaggcgc aggcgcaaac ccggcgtaaa gatgttgcca
    15301 gcgaggccga tttctacggc gcgatggacg gggcatcgaa gtttgtgcgc ggggacgcca
    15361 tcgccgggat gatgattctg gcgatcaacc tgatcggcgg cgtctgtatc gggatcttca
    15421 aatacaacct gagcgccgat gctgccttcc agcagtatgt gctgatggcc atcggcgacg
    15481 gcctggtggc gcagatccct tccctgctgc tctccaccgc ggcggcgatt atcgtcaccc
    15541 gcgtcagcga caacggcgat atcgcccatg acgtgcgtaa ccagctgctg gcaagcccgt
    15601 cggtgctcta caccgccacc gggattatgt tcgtgctggc ggtggtaccg ggaatgccgc
    15661 acctgccgtt tttgctgttc agcgccctgc ttggttttac cggctggcgg atgagcaaac
    15721 agccgctggc ggcggaggcg gaagagaaaa gcctcgaaac gctgacccgc accatcaccg
    15781 aaaccagcga gcagcaggtc agttgggaaa ccattccgct gatcgagcct atcagtttaa
    15841 gcctcggtta caaactggtg gcgctggtgg acaaagcaca gggtaacccg ctcacccagc
    15901 ggattcgcgg cgtgcggcag gtgatttctg acggcaacgg cgtgctgctg ccggagatcc
    15961 gcattcggga aaacttccgc cttaagccca gtcagtacgc cattttcatc aacggcataa
    16021 aggctgatga agtggatatt ccggcggata aactgatggc cctgccctcc agcgaaacct
    16081 acggcgagat tgacggcgtg caggggaacg acccggcgta cgggatgccg gtcacctgga
    16141 tccaggcggc gcagaaagcg aaggcgctga atatggggta tcaggtgatc gacagcgcca
    16201 gcgtgatcgc tacccatgta aataagattg tgcgcagcta tattcctgat ttgtttaact
    16261 atgatgatat tacgcagttg cataaccgtt tatcgtcgac ggcaccgcgt ctggcggaag
    16321 atttgagcgc ggcgctcaat tacagccagt tgctgaaagt gtaccgggcg ctgctggcgg
    16381 ccgatgtgcg cctggcattg cggcgcagca ttacccatcc gttcgttcgc aagcaggagc
    16441 tgacggtgta tacgctgaat aatgagctgg aaaatctgct gaccaacgtg gtgaatcagg
    16501 cgcaacaggg cgggaaagtg atgctcgaca gcgtgccggt cgatccgaat atgctcaacc
    16561 agttccagag tacgatgcca caggtgaaag agcagatgaa agcggcgggg aaagacccgg
    16621 tgctgctggt gccaccgcag ctgcgccctt tgctggcgcg ttatgcaagg ttgtttgcgc
    16681 cggggctgca tgtgctgtcg tataacgaag tgccggatga gctggagttg aagattatgg
    16741 gggcgttgat gtaagtatcc agcattggtg cacaactgcc ggatgcggcg tgaacgcctt
    16801 atccgtccta ccgttcaggc acaagtttgt aggcatgata agacgcgcca gcgtcgcatc
    16861 aggcatctgc gcataactgc cggatgcggc ataaacgcct tatccggcct accgatccgg
    16921 cacaagtttg taggcatgat aagacgcgct agcgtcgcat caggcatctg cgcacaatta
    16981 tgaaatgata cgctccccgg cactaaaatc cgttacgtcc cccgcccaat cgatgggata
    17041 taaccctcgc gcgacatcgc gatggaacgt tgagaatggc caatcactca cttgctttac
    17101 ccaaccatgc tttactggat ttatataaat ataatcaaca tgatgcctat aatctttggt
    17161 attgcggatg gcgtgctccc aaaaacgtgg ttgccagata tttttcaatc cacaagcatg
    17221 ggtaaattgc tttttaattt cccgccagcg cgaggaaaaa tcatcatcgc cttcaggtaa
    17281 tgtccagata cagtgcatat gctctggcaa aacgacccag gcgttgattt caaaaggcct
    17341 gtctcgctta actttaataa tggcgtgacg gagcatctgg tactgggtgg tcaaaagttg
    17401 gcttcgacga tttcgtaaat tcaccgtgaa aaaccatgtt ccccccttga tgtaataacg
    17461 acgatattca gacatggcga ctcctttttc tcaacggtaa gaagaaaagg aaaggttggc
    17521 aatgaggttg ttttatatcg ggaaagcggc tcgcaaatat tcgcgggctc gtaggcatga
    17581 taagacgcgc cagcgtcgca tcaggcacct gcgtacaact gccggatgcg gcgtaaacgc
    17641 cttatccgtc ctacaaaatc gtctcttccg tcaaaatcct gcgcgcgccc aaaaaatggt
    17701 cctgccagaa aggttcggct aatcggctta tccgaatggt ttcgccggta cgtggcgact
    17761 cgataaattg cccatcgccc aaatacacgc ccatatgatc ggctatctcg cggctgtgga
    17821 tatggaaaaa cagcaaatct ccccggcgca ggtcgttgtt cgccacaatc gttgcccggc
    17881 gatagtggta catctcattg gccgtgcgcg ggagcttagc ctcaaggatc ttgttgtagg
    17941 cataaaaaac caacccgcta cagtcaaagc ctttatcagg ccgcgtaccg ccccagacgt
    18001 aaggcttgcc aagctgctgc tccaggcggt gaatcgccac ttcggtaatg ttatgcagat
    18061 ggtcgctgct taaaaagtgg ttgttttccg ccagcgcctg ccagcgtctg tcactcgcct
    18121 tgagcggagc aggaaaccat tctggatgct gttttatctg ctcccggttg tgctggcgta
    18181 gcgccctttt gctttcggca ttgccttcca caatatagct ggcctgcttt ttcagatgag
    18241 tttggtactg ttttaataaa cgcgcacggt tttgcatccg ctggcgggcg gcgtagctga
    18301 aagagatatg tgatgtggtg gcgctggcaa atgaagaaaa cggcaaacaa atcaacagta
    18361 atcccgacaa tacaaaggat ggaatcgacg gcaaggacat actcaataat ggctggtcat
    18421 gctttaatgt tgcagtgtat cccggatcgt ttctgaacaa acttaataaa ccattattat
    18481 aaaattaaca tatatatttc agttaattat gtaaaaacat ccgtttatta aattactcat
    18541 ggattgtcgc tgttgctcat ttgagctaca attcaagctg aataaatata cagcacagga
    18601 gataccccaa tggctgctaa cgcgtttgtt cgcgcccgaa tcgatgaaga tctgaagaat
    18661 caggcagcgg acgtactggc cgggatgggg ctgaccatct ctgacctggt tcgcataacc
    18721 ctcacaaagg tcgcgcgtga aaaggcattg ccgtttgatt tacgcgagcc taatcaatta
    18781 accattcaat caatcaaaaa cagcgaagct ggcattgatg ttcataaggc caaagacgcc
    18841 gatgatttat ttgataaatt aggaatttaa atatgattca aagggatatt gaatactcgg
    18901 gacaatattc aaaggatgta aaacttgcac aaaagcgtca taaggatatg aataaattga
    18961 aatatcttat gacgcttctt atcaataata ctttaccgct tccagctgtt tataaagacc
    19021 acccgctgca aggttcatgg aaaggttatc gcgatgctca tgtcgaaccg gactggatcc
    19081 tgatttacaa acttaccgat aaacttttac gatttgagag aactggaact cacgcggcgc
    19141 tctttgggta aagcccgctt ccgatgaaat ttgtaaaccc tgcgccgaca ggcttgcgta
    19201 aaactctttt ggtgataatg tgagccttcc aaatccagac tattccgatt tgggatccat
    19261 tttgccgtcc tggcgtgtgt aaccgtttta tcaaggaata agcagtatgc gtaaaatcgc
    19321 attaattctt gcgatgttgt tgatcccgtg cgtttcattt gccggtttgc tgggcagcag
    19381 tagctctacc acgcctgtga gcaaagagta taagcagcag ttgatgggat cccctgtcta
    19441 catccagatc ttcaaggaag aacgtacgct cgatctctac gtcaaaatgg gcgagcaata
    19501 tcaactgctc gacagctata aaatctgtaa atattccggc ggcttagggc caaaacagcg
    19561 tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc cagcgtaatc agttaaaacc
    19621 agacagccgt tactacaaag cgattaatat tggtttcccc aatgcctatg accgtgcgca
    19681 tggttatgaa gggaaatacc tgatgatcca cggcgattgt gtttccatcg gctgctacgc
    19741 aatgaccaat cagggtattg atgagatatt ccagttcgtt actggtgcgc tggtgtttgg
    19801 tcagccgagc gtgcaagtga gtatttaccc gttccgcatg accgacgcca atatgaagcg
    19861 ccataaatat tccaacttta aggacttctg ggagcaactg aagccgggtt acgactactt
    19921 tgagcaaacc cgtaagccac caaccgtttc tgtggtcaat ggtcgttacg tggtcagcaa
    19981 gccgttaagc cacgaagttg tgcagccaca actggcatca aactacacgc tccccgaggc
    20041 aaaataagcg ccattcgcct ggcataatct tttgccaggt ttcattgccc gtcagcggct
    20101 gtgttgcaat caccgtgacc acatcattcg gtgtggtctg cgagctgaag tcgatttcca
    20161 catcctgatc cagcaacgtt gccacgccaa acggcgcgcg gcgggtgatc cagtgtaaat
    20221 tagtcgagca atacgccatt acatagcgcc cgtccgaaag cagcatgttg aaaacgccct
    20281 tctgccgcag ttcatccgcc agtgaggcga tatatttaaa taccgccgcc atgttgcccg
    20341 gtgtgcgcgg gtaacgctgc gttaatttat gcaggagcca gcagaaggct ttttcgctgt
    20401 cggtttcgcc taccgggcgg aagttgccgg tttccagtga tttgtagccc gtcagttgtc
    20461 cgttatgggc ataagtccag ttacgccccc ataactcgcg ggtaaatggg tgagtatttt
    20521 ccagcgccac ctcgccccga ttagcctggc gaatatgagc caccaccgaa caggatttta
    20581 tcgggtagtc ctggacaagt ttggcgatgg gggaattaaa gctgggttgt ggatctttaa
    20641 atgtgcgaca gcctttacct tcgtaaaagg taatgcccca gccatcttta tgtggcccgg
    20701 ttccaccacc acgctgtaca agcccggtga aactaaagca gatatcggta gggacgttgg
    20761 cgctcatccc gagcaattcg cacatgggta acctccacaa cagcgggagc gcatggcccc
    20821 cggcgaagat tgatttaaga attttcaggt cggatgcggc gtgaacgcat tattcggcct
    20881 acggttcgcg tacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatcag
    20941 cgcacaaatg ccggatgcgg cgtaaacgtc ttatccggcc tacgccagac ttacttaacc
    21001 atctcttttt caatcaactg gatcaggata tggatcactt taatgtgaat ctcctgaatg
    21061 cggtcggcat aaccaaagtg cggtacgcga atttcgatat ccgccgtgcc agccattttg
    21121 ccgccgtctt taccggtcag ggtgatcact ttcattccct tctcacgcgc cgctgcgatc
    21181 gctttgatca cgtttgcaga gttaccggag gtggagatcc ccagcagtac atcgccttcg
    21241 cgacctaccg cttcaacgta gcgggagaaa atatcattga aaccgaaatc attaccgacg
    21301 caggaaatat gactaacgtc agaaatagca atcgccgggt agcccggacg gttttcacgg
    21361 tagcgaccgg tcaactcttc ggcaaagtgc atagcgtcgc aatgggaacc gccgttgccg
    21421 caggaaagca ctttgccacc ggctttaaag ctgtctgcta acaggaccgc cgcgcgctga
    21481 atggcgtgaa tattggcgtc atcttttaaa aagttagcca gcgtttccgc cgcttcgttc
    21541 agttcgttac gaataagatc ctggtacatg aggatatcct tcagcataaa tgtaatagac
    21601 aaaatgcagt gtaccggata ccgccaaaag cgagaagtac gggcaggtgc tatgaccagg
    21661 actttttgac ctgaagtgcg gataaaaaca gcaacaatgt gagctttgtt gtaattatat
    21721 tgtaaacata ttgctaaatg tttttacatc cactacaacc atatcatcac aagtggtcag
    21781 acctcctaca agtaaggggc ttttcgttat gatgattttg agtattctcg ctacggttgt
    21841 cctgctcggc gcgttgttct atcaccgcgt gagcttattt atcagcagtc tgattttgct
    21901 cgcctggaca gccgccctcg gcgttgctgg tctgtggtcg gcgtgggtac tggtgcctct
    21961 ggccattatc ctcgtgccat ttaactttgc gcctatgcgt aagtcgatga tttccgcgcc
    22021 ggtatttcgc ggtttccgta aggtgatgcc gccgatgtcg cgcactgaga aagaagcgat
    22081 tgatgcgggc accacctggt gggagggcga cttgttccag ggcaagccgg actggaaaaa
    22141 gctgcataac tatccgcagc cgcgcctgac cgccgaagag caagcgtttc tcgacggccc
    22201 ggtagaagaa gcctgccgga tggcgaatga tttccagatc acccatgagc tggcggatct
    22261 gccgccggag ttgtgggcgt accttaaaga gcatcgtttc ttcgcgatga tcatcaaaaa
    22321 agagtacggc gggctggagt tctcggctta tgcccagtct cgcgtgctgc aaaaactctc
    22381 cggcgtgagc gggatcctgg cgattaccgt cggcgtgcca aactcattag gcccgggcga
    22441 actgttgcaa cattacggca ctgacgagca gaaagatcac tatctgccgc gtctggcgcg
    22501 tggtcaggag atcccctgct ttgcactgac cagcccggaa gcgggttccg atgcgggcgc
    22561 gattccggac accgggattg tctgcatggg cgaatggcag ggccagcagg tgctggggat
    22621 gcgtctgacc tggaacaaac gctacattac gctggcaccg attgcgaccg tgcttgggct
    22681 ggcgtttaaa ctctccgacc cggaaaaatt actcggcggt gcagaagatt taggcattac
    22741 ctgtgcgctg atcccaacca ccacgccggg cgtggaaatt ggtcgtcgcc acttcccgct
    22801 gaacgtaccg ttccagaacg gaccgacgcg cggtaaagat gtcttcgtgc cgatcgatta
    22861 catcatcggc gggccgaaaa tggccgggca aggctggcgg atgctggtgg agtgcctctc
    22921 ggtaggccgc ggcatcaccc tgccttccaa ctcaaccggc ggcgtgaaat cggtagcgct
    22981 ggcaaccggc gcgtatgctc acattcgccg tcagttcaaa atctctattg gtaagatgga
    23041 agggattgaa gagccgctgg cgcgtattgc cggtaatgcc tacgtgatgg atgctgcggc
    23101 atcgctgatt acctacggca ttatgctcgg cgaaaaacct gccgtgctgt cggctatcgt
    23161 taagtatcac tgtacccacc gcgggcagca gtcgattatt gatgcgatgg atattaccgg
    23221 cggtaaaggc attatgctcg ggcaaagcaa cttcctggcg cgtgcttacc agggcgcacc
    23281 gattgccatc accgttgaag gggctaacat tctgacccgc agcatgatga tcttcggaca
    23341 aggagcgatt cgttgccatc cgtacgtgct ggaagagatg gaagcggcga agaacaatga
    23401 cgtcaacgcg ttcgataaac tgttgttcaa acatatcggt cacgtcggta gcaacaaagt
    23461 tcgcagcttc tggctgggcc tgacgcgcgg tttaaccagc agcacgccaa ccggcgatgc
    23521 cactaaacgc tactatcagc acctgaaccg cctgagcgcc aacctcgccc tgctttctga
    23581 tgtctcgatg gcagtgctgg gcggcagcct gaaacgtcgc gagcgcatct cggcccgtct
    23641 gggggatatt ttaagccagc tctacctcgc ctctgccgtg ctgaagcgtt atgacgacga
    23701 aggccgtaat gaagccgacc tgccgctggt gcactggggc gtacaagatg cgctgtatca
    23761 ggctgaacag gcgatggatg atttactgca aaacttcccg aaccgcgtgg ttgccgggct
    23821 gctgaatgtg gtgatcttcc cgaccggacg tcattatctg gcaccttctg acaagctgga
    23881 tcataaagtg gcgaagattt tacaagtgcc gaacgccacc cgttcccgca ttggtcgcgg
    23941 tcagtacctg acgccgagcg agcataatcc ggttggcttg ctggaagagg cgctggtgga
    24001 tgtgattgcc gccgacccaa ttcatcagcg gatctgtaaa gagctgggta aaaacctgcc
    24061 gtttacccgt ctggatgaac tggcgcacaa cgcgctggtg aaggggctga ttgataaaga
    24121 tgaagccgct attctggtga aagctgaaga aagccgtctg cgcagtatta acgttgatga
    24181 ctttgatccg gaagagctgg cgacgaagcc ggtaaagttg ccggagaaag tgcggaaagt
    24241 tgaagccgcg taaatgaata acggagccga aaggctccgt ttctttatcc gctaattatt
    24301 taaaattaaa gccatccgga tggttttcca ggctgccggt caacgccgcg aacaacaccg
    24361 ttttaccatc aatcgaaagc gcatcgttca cattcagcca ggtgagtttc tcttgcgacg
    24421 ttttctcatc aatagtcgag aacagccccg tcatctgatt agatttctcg gaccacatca
    24481 cagcgatacg ttgcgagcca cagtcatgcg gtttgcacgc gctcatcacc tgatacgtct
    24541 catctcccaa cgttacggtt tgtgcgggag tataagtacc gcctttcatc acccaggcag
    24601 gcagcttatg cccttgtacc atctgattaa atgcagcttt ggtggtttcg ccctttgcaa
    24661 ggctgctaat ggttaaatca tcctgcgcca ttgcactggt ggcgatgacc agagcggcga
    24721 ctgtcgttat tgccttaaac atcattcctc ccgagcttat cctgcccatg ttattaacct
    24781 cctaaagcct ggcgataaca cgataatttg caaatcagat atttccaata tcagcatttg
    24841 ttatcaattt attctgcgct aaggcaactg cctgataagg tgttaaagtt ataaaaccct
    24901 tcgcataaag gagttgtaca ccgtgcctgg tttgaagatt acgcttttgc agcaaccact
    24961 ggtgtggatg gatggtcctg ccaacctgcg tcattttgat cgtcaactgg aaggtattac
    25021 cgggcgcgat gtgatcgttc taccggagat gtttaccagc ggctttgcca tggaagcggc
    25081 agcttcgtcg ctagcacaag atgacgtagt gaactggatg acagctaagg cgcagcagtg
    25141 caatgcgctg attgcaggca gtgttgcatt acaaacggag tctggttcgg ttaaccgctt
    25201 tttgctggtt gagccgggcg gcacggtaca tttttatgat aagcgtcatc tgttccgcat
    25261 ggcagatgag catctacatt ataaagcggg caatgcgcga gtgattgtgg aatggcgcgg
    25321 ctggcgtatt ttgccgctgg tgtgctacga cttacgtttt cctgtgtggt cgcgcaatct
    25381 caacgattat gacctcgccc tgtacgtcgc caactggcct gctccgcgct ctctgcactg
    25441 gcaggcattg ctgacggccc gcgcgattga gaatcaggcg tatgtggcgg gatgcaatcg
    25501 cgtcggcagc gatggcaacg gctgccatta tcgcggtgac agccgggtga ttaatccgca
    25561 aggagagatt atcgctactg ccgacgcgca tcaggcaacg cgcattgatg cggagctgtc
    25621 gatggcagca ttgcgggaat atagagaaaa gtttccggca tggcaggatg cggatgagtt
    25681 taggttgtgg tgagtggggg ttgtgtttaa ggacggggag agtcggggta ttactacgaa
    25741 agcccgctcc ccgcaaggac tgacgccagg tagtttctgt ccatggctgc ttttcgcatc
    25801 ttacgtctta accctgcctt gaataccttc tcattcgtca aaatattaat agcaatgtgc
    25861 cgtatccctg aaaataattc tgctgcgttt cctcttctta ttttgtagtc gtcttcattc
    25921 attaccacgt ccagacgcct gtgcagctta ttctccacgt gccagtggtt tcggattgct
    25981 gtggcgaact tctctgcggt taaatcagca gaactgatat aatatctgac cgtcatttct
    26041 ggctctttct tttgttctgc tattattgac cgaaaggaga ctgccacgca taatttcttc
    26101 agccctttcc attcaaatgt gaaatcaata agttcatcag ggacatcgca aacaatgtga
    26161 agacggattt cttctctgcc gtgactcttt tcactaattg cgtaactgtc atgctctgga
    26221 ttatttaatt ctttcagcgg aaatttttcc tcgaaggctt tatttagccg cccctggttt
    26281 ccttttaccg cgaataaata atcacctccc tgtttttgta tcttctctgc aatatctttc
    26341 tggcaaccca tcgcatcagt tgtgatgatt tttcctttaa tatccagcat gttaagaagt
    26401 tcagggatag ctgtaatctc attggatttc tcatccgtcc tgatctgtcc gatgaccaga
    26461 ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc ccttgcggcg actcttgtca
    26521 taagaatgcc ggagtgtttt tccatcaatt gcgatgacgt ctttatcatc tgaagtatgg
    26581 caatctcgca tccagttaat aaagcactcg tgaaattttg aaggactgat acaggataca
    26641 actctggcga tggtatcgtg aacaggaata ccattttcaa aatcaccata ttgttttaaa
    26701 aaatcgagat gtgtttcccc aaaatcctct atatcttccc agccttctgc accagaaata
    26761 acggcacaaa tagtcaacag tagaatatct gacaatttat gttccacttt ccaggcttgt
    26821 ctgtaatcgg ggataataga aatatgttcc atcaattttt taagttccat tttgttctcc
    26881 ttaattatgt aaggagtatt tgatcatgta taagcaataa aaaacagctt caggtaatga
    26941 ggaatatctc aatttttaaa cataaaatgc gaattattta gtacaaaaaa tcagcgttac
    27001 gtgatctttc cctgcccctg cgctcactac catggctctt taccacagca gccggggttg
    27061 tttgggaccg acttccgaca gcagatcccg gtgggcctgc caccatctct tcacaagctt
    27121 atgcttatta catgcaaaac gtcccgtttt gcatctttgt gcctacggca caccggtgat
    27181 ctttccctga tgagggatat attaactatt gaacattagc gcgaattatt tagtgcaaaa
    27241 aatcagcatc acgtgatgct tttcctgcta ttaataacct cctctattta actatatttt
    27301 tcattgatgc aattcgtaga tttgaagaag ttcaactgcc acatttttat ttttactatc
    27361 gcaattttta taccataccc atgcttccgt attttttact ttttttaaat catccataat
    27421 aaaatcccca ccaaaaacag taaaatacaa tgtttcctca ttaaaaataa aactcactgc
    27481 ccccccctct tttattttaa tttccgcttc atttgtgttc aaatcaactt ttttaattac
    27541 tccatgtaag ttgggttttg atagtttttc tacgccacca tcaggagcgg gtaatataca
    27601 tcgtttaaga actttagcat ttgcacatcc aataacccaa attagaatgc acactcccat
    27661 gttaatcgca ttaacgtgtc ttaaaacgtt catatctaat tctctctcca ggatgcgaat
    27721 acatccaact tgtcactttc ttacacaact catctacatc ttcattctgt gcacgggtac
    27781 atcccatagt tggtttccag ccttgtcgag gagcataagg atcgcgcaac gatgatcccc
    27841 caccatgtag ccatcttgct ctagtatctg tagtatagat cttcgttgtg ccatatgctg
    27901 ccgatattcc tgattcattt gtctcacaac caacgacttc acctgtatat ggatcaccag
    27961 caccacgttg agaacgacga tcaattctat tgctactatc aaaagtcatt gaatcacctg
    28021 tttcaggatc ataataaata gtcactcctc cagtcatatc agtaagaaca agaggagttt
    28081 cagcacctgc ccggtctgga gcgggaggcc catatacact tataactcgt tctaacccca
    28141 gtggatcagt gtaacgtatt ggtaccagtg gatacgtgta tagatttatt ccccccttca
    28201 accctatcgg atcctgggtg atataccgcc cctgcaacgg atcgtagtac cggtgacggt
    28261 tgtaatacag tcccgactcc tcatcatact gctgccccgg caaacggtac ggctggtaca
    28321 ggtgatgcgg gttctcctta ttaagctggt tcccccactc gtcatactcc ccgcaccacg
    28381 ccgtttcgcc ttccggggtg atgagcgcct gcggcagtcc acggtggtca cagtggtaaa
    28441 gatgcagcct ccgctccggg atgtatgcat cctccatctg cgcggccatc tgttccgccg
    28501 tcagcccgca ctgcgcaagc catgcctcac tctccgcact caccgctccc gcacgcagct
    28561 cccgctccag ccttcccagc atcaccgcca gctccgccgg tagcgtcaca cccgtgtcct
    28621 cctgcaacac ctccgccagg ctccggtgcc gcgccttcgc ctgttcgcca ttctctgttt
    28681 cgattctcag gagcggcgtg aagctccccg gctggtatac cgtctggata cgcgtggtgc
    28741 cggtctgtat cgtcgtcagc ctgtcaccgt cccacccgta ccatgtcacc tccggtttac
    28801 gcgacagcga catccacccc gtcaggtcac gctcccgccg ccatacccgt tttcccgtcc
    28861 ggcggcccag cgggtcatag aggtaccggc tctccacctg tggttcgccg tgctgtatcc
    28921 gcgtgtagaa caccaggcgg tgctggctgt cgtagtggta gtggtgggtc cgctcgtcgt
    28981 gcatccgtat caccccttcc gggatgcggt ccgtcttctc cgccagcctg ccgtattcat
    29041 cgtggcggta gacatagtgc gcatcttccg cgatgcggtt atccggccat gccgtgagcg
    29101 tgctgtccgg gtgcagttcc gggtccggca gccggtttcc tgccgggtcc gtcgcatacg
    29161 ggatatcgat atccaggttc gccgccgtgg tgtggacgcc cgtcagcctt cccgtgtcgc
    29221 tgtaacggta ctcccggctc tcctgcgggc cgctgatgcg caccagttgc ccgttgtcgt
    29281 tccacgtata ctcacggtca agctgcggga ggttcaggtg cctgctccgg agctgccctg
    29341 tgagggtgta cgccgtggcc tgttcgtacc ccgctgtact gcccgccccg ccgaagctgc
    29401 gggccgtctc acggtgcagc ctgtcccgcg tgtactcgac cagcggcgtt ccgcccagct
    29461 tcatccccgc aagataaccg ctgccatatg tcagccactc caccggcggc agaccgtccg
    29521 gctcctgacg ggtcgccagc ccctgttcgc tgtacgcgtg ccccgtctca tgctcccaca
    29581 gcatctcccc cgtctccggg ttctccaccg tctgccgttc gcccgtcagg cggcctttat
    29641 cgtcatagcc gtagtggacc gccacacggt ggccttcact ggtatggctg atggtggtta
    29701 accacccgtg ctcatcatac tgccactgct ccgccgggtc gccgttcacc gtacggtgcg
    29761 tgatgcggtc cgacgcatcg taatgccaga gggtgataag cccctcatct tcactctgcg
    29821 tgagttttcc ggtcaggtca tactggtaac gttgcgtccg gccgtcaaag ccgcgctgct
    29881 ccgtcagcct gtccaccggg tcataccgga acgtggactg gctgccgttc tcgttagtca
    29941 gcacggtgat gcgcccggcg gcgtcatacc ccatgctgcg cgtcagtcca ccttccgtca
    30001 cactcaccgg acgcccacgt ttatcatatt cggtggcgct gcgtttaccg tccggtgata
    30061 tcgttgctgt caggtcgcct gcagcgctgt actcataacg ggtttcacgc ccctgcgcat
    30121 ccttccgact gatcagttgg ccacgcgggt tataactgct gtaagtgctg atgccttctt
    30181 cccggtgaac ggcgatttgt tgaccgtact ggtcgtactc ataccgcgtc gtgtaccccg
    30241 agcagtccgt aaaggccagc agttgaccgt aacggtttcg cgttattctc cgcctcaccc
    30301 cggttccgtc ttccaccgca cagggcaggc cactgcggga aaaatcataa aaccagcgcg
    30361 taatgttacc gttgcgcgag gtctcttctg ccagtcggcc ctgacggtca tatttccggc
    30421 tgctgcgtaa accatccgga tatgtcactg aagtcagttg caactggctg ttatagccat
    30481 accgcaccgt cctgccgtcc ggcagtatca ctgaagtcag ctttccagac gccatatgca
    30541 gccggtactc cgtccgccgc cccgccgcat ccgtctgcgc cttcagcctc cccgcctcat
    30601 catactcgct gcgggtgatg ctcccgtcgg catgctcctt cttcaccacc cgcttcaggc
    30661 caccctcgcc ttccgtgtac agcacctccc gccggttcag gctgtccgtg atgattaccc
    30721 ggctctctcc gtactcaaag cggtagtcca gcccctccgg gttgacctgc tccgtcaccc
    30781 gaccggtatc atcataccgg tagcggctct ccggcctccc cgcataatgg tgcgccacca
    30841 tccgcccggc gtgctccgca tcataagtaa acccgcgaac ctgcgtcccg ctgcggtcat
    30901 acaccgcccg cagttctccg ctggccgtgt acgtgtagcg cgccagcggc gcagtcggct
    30961 gttcatccgg gtatgccggg tcgtgtgtca gccacaccgc ctccagccgg ataccgttat
    31021 cggcaccgta ctctgtaccg gcgggcagcg tgtcggggaa aaactgagaa gaggaagcag
    31081 aacggggacc ggctggagaa gataaggaag actcgcgctg tttacggaag gcttctgccc
    31141 gctgcgcctg tgtgctcagc accaggtgaa aacagcggcc cgcaccgtcc gtcacccccg
    31201 tcaccgcgcc cgccacgtca ccctccgccg cccggtggaa agtcagcgtc cgcccgaagc
    31261 cgtccaccac gcccgtcagc acccggtatg cgggcggctc cggcggcggc acctcgtccg
    31321 cctccggcac acgctccggc cagccgagta tcagccactg cccggttgtg ctgacagcca
    31381 tcatataggt gtgtggactc agacggaccg cttccggcaa cgcccgccag agccgcgcaa
    31441 gcggatggcc tttgtgttgt ttcagcactc cgccccttgc cagccagaac gactcgctgc
    31501 ggctgtagct tatctcgccg ggaaacagcg actcaaagtg aatgctcctg ccgccgctgt
    31561 cgttgagtat cagttcccgc tcatgaacct gtaagcggat atcgaagggc gctttccagc
    31621 cgggaccaaa cacgcccact ggcgcgggcg tcctggtccg gtagctgctg taggcgcgga
    31681 aaagaataaa cggcagcggg ccgggcagtg ccaggtcggt ttcaacaggc agaaccttcg
    31741 caccgagtag cgagttcacc gggctgccat agttcggact gtcctttttt gtgggacaca
    31801 ccgagcaggc cacaccggtt ggcgcaccta tcagcacccc tgctgaaccc tgcacaatat
    31861 ccagtccctt gcgggtcatg tcaccctgcc gcgccgccgg ttttccgccg cccatgaatc
    31921 aattccttct gcttttaata tggttcatct ggtcactgca ccagttccgt tttaattttt
    31981 tgcacctgac tcaaaattgc cgcatatccg gcattccggg gggtaaatct ttcggcacag
    32041 gcgcatccgg tacaggttgt gtggcaccac agttccagtt aatcactggc gcatcaccgt
    32101 tcacatccga ctccccttcc agatgaatgt gcgtaccgtt gagaaatatg cttccgtcct
    32161 tcgtcagcac cagccttgcc ttaccacagc agagttcaag atgctcaccg gcagaataaa
    32221 ctgctgtttg tccggtgttt tccttcatcg tcttgcccac cgagaaggtg acgttattcc
    32281 ccacattgac gctgtagccc attcccacca tcagtgattt atgcagccct acctgtgagg
    32341 actgcaacaa cgccaccgag gtattcataa tgccgcctac cgtcgtctgg taagccacac
    32401 ccaccgtcag cgcacgaatg atccccacct tttcaacctg gtttgatccc actttgataa
    32461 tctggttact acccaccgtt tccacctggt tgacaccaac cgtctgtatc tgattaacgc
    32521 caatgttctg gatctggtta ttggtaacgg tgatcgcctg gttgttacct attttttcgg
    32581 catggttgtt tatcacatca gtggtgcggt tattcagcac ctcggtgttc atgttcttct
    32641 gcgcgtggat gtagacctgt tctttccctg tcgcatcgtc aaacttcagt tcattaaacc
    32701 cgctgccctt ataggttttc gaacgaatgg tcatctgcgt ctttgttccc ggcaggctgc
    32761 cgggtgtgcg gttttcctgg tggtaggtgc gccccataat gatcggctgg tccggatcgc
    32821 cgttgaggaa gtccacaatc acctcctgac ccacacgcgg tatcgccagg ttaccgaatc
    32881 cggtgcctgc ccacgcctgt gccacacgga tccagcatga actgtcctgg tttgacgggt
    32941 tataacggtc ccagttaaat ttcacccgca cgcggccatg ttcatcacag aagatttcct
    33001 cgcctgccgg tcccgtcacg acggcgctct gcgggccgtc caccagcggt ttcagcaacg
    33061 gctgtggccg ccatgtccgg tctgccggta ttaccgcaaa gtggttattc agcgtggttc
    33121 ctgaaccact gcgtcccggc accgcctgcg gctgctcgcc gtgcagttca cttgccacca
    33181 cctgccattc ccggttcagg ttcgcctgcg gatgccccgt cagcacaatt cgccgtcccg
    33241 gccatatctc cggagaacgg cttgttccgc gcgccacttc tgcgttgttg cgccagccat
    33301 ccatctgcca gcgggcaaag ttctgcccgt gagcaccctt gaaacgtccg gggtagtcat
    33361 acacttcata ctgtgtgcgc tggtaatcct ggtactggcc ttcctgatca aaacgccctg
    33421 cccagccggg tcgtttaaag gtgtagtctt tggtcaccac ggaagaaggg cggatttgtg
    33481 cgctgtagcg aaactggctg atgcagaggg tgcttacctc ggtacgggtg ttcggattcc
    33541 aggggatctc aaaggactcc ggcagataac gcacggtatc gcacaggacc aggctctggt
    33601 cggtactttt ttgcgcgtgc tcctcataaa agaagatgcc ttcctccgcc gccatccggc
    33661 acaggaaatc gtaatcagtc tcaccgtact ggacacaaaa ctcacgggaa ggatgtggct
    33721 cgctgaacag cgggctccac tcggtcaccc cgttttcctg caatatcgtg ccgaggatgc
    33781 tttcgatgtc ctcattctgg aagatacgga agttctggcg cagccctgtg cgccacagcg
    33841 gcgggcatac cttcatgctg tacagcttct ggtttttgtc gttctccccc agttcaaacc
    33901 aggtcaccac acctttcacc cggcgctgta cgtcatcgcc ctgccatatc gtcaggtagg
    33961 ccattttgtc gaggatctgc tggaattcaa gggagagaaa ctgctggctg accagagaga
    34021 gatcgaggga aaaaagcgaa gagagtgact ggttcagatg aaaggaaacc accgcaaaag
    34081 catccggtgg caggccgtcc acttccagcg tgaaacgtaa tccggttgac atagcaacct
    34141 ccggggttaa ggaaaaacag acagttcgct gttaaagcgg ctgtgacagt acagtgttac
    34201 gcttgcgttc aggcacgcta ccgccgaagt ttccggcagc cgccatctcc cgtgaacgta
    34261 accccttact gacaaccttt ctttctacgg cgtaatacaa aaacagcatc cggcctgata
    34321 gccggatgct gttggcttaa cttatgcttc cagcggtgcg cgccagtcgt cggcaccgga
    34381 ggtgccagat ttgacgtgtt cccattcaat tttgcggtag gccagtgaaa cttcgagcaa
    34441 ctgggtgaac tcgcgtttcg ccgggtcctg gcaatgcggc atatgcagtt tcatatcgac
    34501 aatcgtcgaa tcagtcaggc gggtagtgaa ataatgctcc tgtttgcctt ccacagaggt
    34561 acgccaccag tgaagttcag tggttggcag catttcgccg gaggccagtg cgttgtacat
    34621 cagcggaacc gctttgttca gcgccacggt aaaaatgaaa ggcttgtgag cacgctgacc
    34681 cgcaggctga ccggactgag gatcggtcgg gacagtaacg ttatgcagga attcctgcac
    34741 cagcatctcg tcttcgtgcc cctgcacata gatgttgccg acagaatcag cggtaaatgc
    34801 acctgcagta atatttccct gggtctgacc agtgatggag atataacacg gtgttggcat
    34861 ttgtaaactc cttgttaacg tggttaatta ctacttacaa tgacctgccg ttaatcccag
    34921 acaggcagat gcagctcact tcacggaacc gcatcatttt cagtattttt tacatacaaa
    34981 ccgagctaga atttacggtt gtcgtaaaaa ctatttttaa aatcattaaa tctcttattc
    35041 agcaataact taataatata tatctggata accataccca actgttcaaa gtccgtattg
    35101 cctccataaa atggccaaag ctgaatcatg aataaatatt gtatttatcc tttctgaatt
    35161 atca
//
LOCUS       DBBGLQ010000045        34464 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.045, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000045 DBBGLQ010000000
VERSION     DBBGLQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.045"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..490
                     /locus_tag="ACIYSB_002881"
     CDS             <1..490
                     /locus_tag="ACIYSB_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5429892.1"
                     /translation="ARTVELRLSEIGEWDFTEMLWTVPKEHSKTKVAIFRPIPEAILP
                     FVTRLVEQNRNTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSLHDIRRTFTTMLNDLG
                     VDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWVERLEILAGAHENVTTLPVTR
                     RK"
     gene            865..1053
                     /locus_tag="ACIYSB_002882"
     CDS             865..1053
                     /locus_tag="ACIYSB_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5429893.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKESCEAVH"
     gene            1106..1240
                     /locus_tag="ACIYSB_002883"
                     /pseudo
     CDS             1106..1240
                     /locus_tag="ACIYSB_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1378..1557)
                     /locus_tag="ACIYSB_002884"
     CDS             complement(1378..1557)
                     /locus_tag="ACIYSB_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGC5429894.1"
                     /translation="MATGSTNNKSQQLNARFPHDVVADLEKNLDEGESKAQFIVTAVK
                     GEIKRRQRRKAKEAE"
     gene            1742..1939
                     /locus_tag="ACIYSB_002885"
     CDS             1742..1939
                     /locus_tag="ACIYSB_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGC5429895.1"
                     /translation="MAGTQHTPRLAHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEGSHE"
     gene            1932..2159
                     /locus_tag="ACIYSB_002886"
     CDS             1932..2159
                     /locus_tag="ACIYSB_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429896.1"
                     /translation="MNKIPFDVLVHSENALIRAKEMDALLLNLIHVQESGDESDSMMF
                     SVVRTLLTAVINELNTVMKIHENNKAQHTGE"
     gene            2165..2452
                     /locus_tag="ACIYSB_002887"
     CDS             2165..2452
                     /locus_tag="ACIYSB_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005765007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGC5429897.1"
                     /translation="MKNKNSGVNASGPARPEIRPGDIFRDNYGGTVMIKGVEERRITY
                     RREGYEYDCVMPVYQFRRDFTLVGHRKAVNQKRAASYIRKIREMLVAGGKK"
     gene            2449..3855
                     /locus_tag="ACIYSB_002888"
     CDS             2449..3855
                     /locus_tag="ACIYSB_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGC5429898.1"
                     /translation="MKLAPNVKLLPKDKHTEAVIFAGDNAHSFAEHYMIAQAKKAGDP
                     IPPVYLGRYQLSELDNLQIVDEGRYRAKVIRAGNLDDMQMLTIATKLAIAGVQEARLL
                     SENFELLEDWSDQLPRLRETWERGESLVMNGGKRKITLPISWGSEGFDAQQSYVIKGL
                     IPAESLCSTYGASGSYKSFLAISWSCHVATGMAWAGRRVSKGAVIYIAGEGSMGVKRR
                     VKAWEITHDKVVTDLCIINAPVFPASPDYVEQVIRTAGLVKSRTGENVRLIVIDTLAR
                     CFGGNDENDSRDMGAFIQGCDAIKQATGATVLVVHHSGKDETKGARGSSAFRAALDAE
                     YRISRENSDVTALVAACTKMKDAEEPKESAYDLKSVEVFTDTDGEEIVSMVVIDVPRA
                     PAELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKRLGYDVKNMRRWLA
                     KLEKDAVIYIDGDDVGPL"
     gene            4102..4425
                     /locus_tag="ACIYSB_002889"
     CDS             4102..4425
                     /locus_tag="ACIYSB_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429899.1"
                     /translation="MNTEIETLSISNALPGWWAKFKDDDGTEWYSPVAAWALCEIHYF
                     GTGDTYREILPVLTSELGMSPHSPDEGMCECLYLPDKKFVHCGESMVFAWYPVNDSSN
                     SGTLE"
     gene            4429..4671
                     /locus_tag="ACIYSB_002890"
     CDS             4429..4671
                     /locus_tag="ACIYSB_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429900.1"
                     /translation="MKVNHKKTDHRAIDLTEHWLRVAIKIIDRNTGEGYAKAHPELIS
                     AFMTTTAANFATLTERDIAEAEQVTTINVKTGEQTA"
     gene            4668..5078
                     /locus_tag="ACIYSB_002891"
     CDS             4668..5078
                     /locus_tag="ACIYSB_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGC5429901.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGSRRTTTGTQGNQPPAGGDDPYGDDIPF"
     gene            5089..5370
                     /locus_tag="ACIYSB_002892"
     CDS             5089..5370
                     /locus_tag="ACIYSB_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGC5429902.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMMLVETDKEREQVAKRRA
                     ECIRKAARQPVIPDNFGILKEAINRTHTGMCLQKPGGEMFRNYPKKRDN"
     gene            5416..5664
                     /locus_tag="ACIYSB_002893"
     CDS             5416..5664
                     /locus_tag="ACIYSB_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429903.1"
                     /translation="MPVTFEEVQQHKKFHGFDDLETTTAKKYRRLLSSDALFFVDHND
                     FLRSSLTGEIFATNREQVEAMIEYLWKIRRRMRDPVKR"
     gene            6067..7224
                     /locus_tag="ACIYSB_002894"
     CDS             6067..7224
                     /locus_tag="ACIYSB_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGC5429904.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPKINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKSDKERDFGTLQVIKPAHGLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNITEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            7264..7836
                     /locus_tag="ACIYSB_002895"
     CDS             7264..7836
                     /locus_tag="ACIYSB_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGC5429905.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGKLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWVDLAGV"
     gene            7838..9055
                     /locus_tag="ACIYSB_002896"
     CDS             7838..9055
                     /locus_tag="ACIYSB_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGC5429906.1"
                     /translation="MWLFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKAALLVASPVPGT
                     RYLVEFDSADLLRATPTERYATYEKGIKNGIINPNEAREREGMPPREGGDEFSQAWKQ
                     EVTISKNSKGGDE"
     gene            9052..9390
                     /locus_tag="ACIYSB_002897"
     CDS             9052..9390
                     /locus_tag="ACIYSB_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGC5429907.1"
                     /translation="MIAGKMRDRVTIMTFTTQRLPSGAIQQIWTEGETIWAEVKGISG
                     RELLTAGAETAPATIRVWVRYRGDISAASRLKVLTGAFAGSTLNIIGIPLPDAKRTRL
                     EILAKVGTEK"
     gene            9387..9683
                     /locus_tag="ACIYSB_002898"
     CDS             9387..9683
                     /locus_tag="ACIYSB_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGC5429908.1"
                     /translation="MTAEKITLEEAKLHCRIDGNTEDTLIQAYISAALEVCQKHIGRR
                     FDDGLEFTPAIKIGCLMYISQLYEYRTTLGDTDTKEIPLTISALWSVYRDVGVY"
     gene            9683..10123
                     /locus_tag="ACIYSB_002899"
     CDS             9683..10123
                     /locus_tag="ACIYSB_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGC5429909.1"
                     /translation="MPWQPLRRCTQPGCNRRVKSGKCEEHRRSARQQQDSRRGSSRER
                     GYTRQWEKYRAMYLSKNPLCAHCLEKGIYTPAVVVDHIIPIDGGNDVLFWPEWNHQAL
                     CQTCHNRKTTREDPATKANRKAGMYREQEERAAHRNDWMYGDDD"
     gene            10107..10289
                     /locus_tag="ACIYSB_002900"
     CDS             10107..10289
                     /locus_tag="ACIYSB_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429910.1"
                     /translation="MAMMTEQEQTRLIRGLIRQRDTWKTQETEHKANRTGRTKRTTAK
                     RLTDRDREVMECFRNR"
     gene            10414..10770
                     /locus_tag="ACIYSB_002901"
     CDS             10414..10770
                     /locus_tag="ACIYSB_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGC5429911.1"
                     /translation="MARPPKAPAYLDEIAVRQWKEKSRQLSGREDLTPADWSNLELYC
                     VNYSIYRKAVEDLATRGFSIVNSQGSESRNPALSAKADAERIMIKMASLLGFDPVSRR
                     RNPPETEEEDELDRLA"
     gene            10754..12415
                     /locus_tag="ACIYSB_002902"
     CDS             10754..12415
                     /locus_tag="ACIYSB_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGC5429912.1"
                     /translation="MTAWHEYAEGVKNGKITACKRLKQAVKRYFSDLENPLYTFDPEV
                     VERFIAFSRVCPHVKGAMRGSPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTVAAILANWFLVMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRKRVNI
                     QAHKVIHAKTNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMLTKRFNIWCQGATPWMGNGAWAECAGT
                     FAEADLYGQECYAGLDLSSTSDISSVCYAFPVGKKIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWVKAGWIRTTPGDCIDYDRIRDDIMADAENFSIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRKVIVHRGDPVLAWSMSNVVMQSDANANIKPNK
                     KKSSNKIDPSVAALMAFGTFQAEHEEFAFDMSDSHKERLAAFDGV"
     gene            12421..12702
                     /locus_tag="ACIYSB_002903"
     CDS             12421..12702
                     /locus_tag="ACIYSB_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429913.1"
                     /translation="MSEAELLGLIRRVTGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRDIRIRVLELLAYRRRVAMYQESAKNTYQWKKPERLRR"
     gene            complement(12805..13095)
                     /locus_tag="ACIYSB_002904"
     CDS             complement(12805..13095)
                     /locus_tag="ACIYSB_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429914.1"
                     /translation="MRMTRHKKQILELYKPEYRDWVRVEAGDPPFDVRGVTVLLYGSE
                     YRRYHIEATRRTLNAMVRDKLLERVKVREPRFDVRIGGDGAHCTVIRYGLVR"
     gene            13210..13512
                     /locus_tag="ACIYSB_002905"
     CDS             13210..13512
                     /locus_tag="ACIYSB_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGC5429915.1"
                     /translation="MVHDDKAEELEAKGLWRRAANRWGELLKQAASDEARQYVTERRT
                     ACIRKATITREPERDLICAIRTAATKTLTDMGIDLKKEDPLRGIQHGHTKQDKRRK"
     gene            13864..14184
                     /locus_tag="ACIYSB_002906"
     CDS             13864..14184
                     /locus_tag="ACIYSB_002906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429916.1"
                     /translation="MFVIPVPLKRNTPFRRSWWTHSHEIKKMRYAGGHDAGLGADVIR
                     TDIETKTNSILVQCKTSLNVVLRPESSQWFYASCVALVGFSTIGGKHGSTSTSHIKHQ
                     QNGG"
     gene            complement(14706..15941)
                     /gene="allC"
                     /locus_tag="ACIYSB_002907"
     CDS             complement(14706..15941)
                     /gene="allC"
                     /locus_tag="ACIYSB_002907"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGC5429917.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(15963..17012)
                     /gene="allD"
                     /locus_tag="ACIYSB_002908"
     CDS             complement(15963..17012)
                     /gene="allD"
                     /locus_tag="ACIYSB_002908"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGC5429918.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGVPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIIDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            17329..18996
                     /gene="fdrA"
                     /locus_tag="ACIYSB_002909"
     CDS             17329..18996
                     /gene="fdrA"
                     /locus_tag="ACIYSB_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGC5429919.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     SRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            19006..20265
                     /gene="ylbE"
                     /locus_tag="ACIYSB_002910"
     CDS             19006..20265
                     /gene="ylbE"
                     /locus_tag="ACIYSB_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGC5429920.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNQGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMEHGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRVPLACFEQALEALAESMGIG"
     gene            20276..21091
                     /gene="ylbF"
                     /locus_tag="ACIYSB_002911"
     CDS             20276..21091
                     /gene="ylbF"
                     /locus_tag="ACIYSB_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGC5429921.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     KGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGTP"
     gene            21088..21981
                     /gene="ybcF"
                     /locus_tag="ACIYSB_002912"
     CDS             21088..21981
                     /gene="ybcF"
                     /locus_tag="ACIYSB_002912"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGC5429922.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(22176..23243)
                     /gene="purK"
                     /locus_tag="ACIYSB_002913"
     CDS             complement(22176..23243)
                     /gene="purK"
                     /locus_tag="ACIYSB_002913"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGC5429923.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(23240..23749)
                     /gene="purE"
                     /locus_tag="ACIYSB_002914"
     CDS             complement(23240..23749)
                     /gene="purE"
                     /locus_tag="ACIYSB_002914"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGC5429924.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELYQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(23867..24589)
                     /gene="lpxH"
                     /locus_tag="ACIYSB_002915"
     CDS             complement(23867..24589)
                     /gene="lpxH"
                     /locus_tag="ACIYSB_002915"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGC5429925.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(24592..25086)
                     /gene="ppiB"
                     /locus_tag="ACIYSB_002916"
     CDS             complement(24592..25086)
                     /gene="ppiB"
                     /locus_tag="ACIYSB_002916"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGC5429926.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            25260..26645
                     /gene="cysS"
                     /locus_tag="ACIYSB_002917"
     CDS             25260..26645
                     /gene="cysS"
                     /locus_tag="ACIYSB_002917"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGC5429927.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADGSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(26681..27202)
                     /gene="ybcI"
                     /locus_tag="ACIYSB_002918"
     CDS             complement(26681..27202)
                     /gene="ybcI"
                     /locus_tag="ACIYSB_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGC5429928.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGIAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GILLMGMLWWHRR"
     gene            complement(27310..27522)
                     /gene="ybcJ"
                     /locus_tag="ACIYSB_002919"
     CDS             complement(27310..27522)
                     /gene="ybcJ"
                     /locus_tag="ACIYSB_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGC5429929.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(27524..28390)
                     /gene="folD"
                     /locus_tag="ACIYSB_002920"
     CDS             complement(27524..28390)
                     /gene="folD"
                     /locus_tag="ACIYSB_002920"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGC5429930.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            28870..29412
                     /gene="fimA"
                     /locus_tag="ACIYSB_002921"
     CDS             28870..29412
                     /gene="fimA"
                     /locus_tag="ACIYSB_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGC5429931.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAVCSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            29632..30324
                     /gene="fimC"
                     /locus_tag="ACIYSB_002922"
     CDS             29632..30324
                     /gene="fimC"
                     /locus_tag="ACIYSB_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGC5429932.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLINSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            30355..32964
                     /gene="sfmD"
                     /locus_tag="ACIYSB_002923"
     CDS             30355..32964
                     /gene="sfmD"
                     /locus_tag="ACIYSB_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGC5429933.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTVLGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            32977..33984
                     /gene="fimH"
                     /locus_tag="ACIYSB_002924"
     CDS             32977..33984
                     /gene="fimH"
                     /locus_tag="ACIYSB_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGC5429934.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGLGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            33995..>34464
                     /gene="sfmF"
                     /locus_tag="ACIYSB_002925"
     CDS             33995..>34464
                     /gene="sfmF"
                     /locus_tag="ACIYSB_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGC5429935.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAVDPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRP"
BASE COUNT         8772 a         8380 c         9291 g         8021 t
ORIGIN      
        1 agccaggacg gtagaactga ggctatcgga gatcggggag tgggatttca cggaaatgct
       61 ctggaccgtt ccgaaggagc acagcaaaac gaaggtcgca atattccggc ccataccgga
      121 agcgatcctg ccgttcgtca cgcggctggt ggagcagaac aggaacacgg gcttattgct
      181 gggggaagtg aaacaggagg ccagcgtatc gcagtatgga agattagcgc acaggaggct
      241 taatcaccct cactggtcac tgcatgacat ccggcgcacc tttacaacga tgctgaacga
      301 tttaggcgtg gatcctcacg tcgtggagca gcttacaggc caccagatgc caggaatgca
      361 gcgagtttat aatcattccc gttatctgga tgcgaaacgc aatgcgctgg atatgtgggt
      421 ggagcggttg gagatactgg cgggagcaca tgaaaacgta accacgctac cagtaaccag
      481 aagaaaataa tttttttcgt gtttttccag tatgcgcata ctggataagt gcacagatac
      541 aacgtgaaac aatacgcaac aacgtgaaac aaccacgaac aagaggccaa aaagtataca
      601 gatttataag ttgctgattt ttaatgctca actggttttt tatacattag cgaataacgc
      661 tttaaatagt gagaaaaaag gcataaaacg agattttatg cctttgcata atactgaatt
      721 aaaatgattt ttattaaaat caccccgttt ctgtaaaata cccgccacca acatttgcca
      781 actctgaatc accctgacac aatcagacac atcccaacgc aatagaacac gaaataactc
      841 tttaagaaac gaaagggggc aatagtgtta agcactgatc gctttatacg tgaaaaagaa
      901 tgcgaaaagc taacaggcct tagccgctca tgccgctacc gcctggaaaa ggccggacaa
      961 ttcccatcac ggcgtaagct gggcggtcgt tccgttggct ggtctttatc cgaagttctg
     1021 gcatggaagg aaagctgcga ggcagttcac taaaaagact ggcggcacac agccgccacc
     1081 catccactaa tacagagatt taaccatgaa gacaggatat acgcccgaac aggggcgggg
     1141 cttcgttcgg cctggtgaaa ctgaaaaaca acaaaatcgc ggtttttcag acataaaaaa
     1201 agcggccccg ataaggagcc gcctttctga acaatttacc tgccgcgcaa aaaatgaata
     1261 tgagcagggg aattatatca accgtgtggc gaagcgccac aattgccgga taacgggcaa
     1321 agaaaaggcc acctgtcacg gtggccaatg tgatcagtgt gctggcttgc tggtggacta
     1381 ttccgcttct ttggccttgc ggcgctggcg gcgtttgatc tcgcctttaa cggcagtaac
     1441 tataaattgc gccttgcttt caccttcatc taaatttttt tctaagtctg caacaacatc
     1501 atgcgggaat ctggcattta actgttgcga cttattattt gttgaacctg ttgccattac
     1561 tgaatcctct ctaagttggt gcgattcagt atacacaaaa aaaatttcag agaaaggctt
     1621 gaagtgcgat tcacttactg ataggttaaa aatgagtggt gcgatgcacc aaacgacaac
     1681 gccccgcagt ggtggcacac atgcagggcg tctaaccaaa ccgttaacag gagtaacgat
     1741 tatggctgga acacagcata ccccacgcct tgcgcacaca caaacggcct ttgtgtggcg
     1801 ttttctggca ctgagtgccg gagaatccca aatcatccac gtcaccgcct ggacggaacg
     1861 cgaagcgcgt aaccgttgcc cgtccggttg tgtcgctgta ttcgccgccc gtattcgtca
     1921 ggaggggagt catgaataaa ataccctttg atgttcttgt tcattctgaa aacgcattaa
     1981 tccgcgcaaa ggaaatggac gcattactac ttaatttaat tcatgtgcag gaaagtggcg
     2041 atgaatctga ttcaatgatg ttttctgtcg tgcgtacgct attaacggct gttattaatg
     2101 aattaaatac agtgatgaaa attcacgaga ataataaagc gcagcacacc ggagaataaa
     2161 aaacatgaaa aataaaaatt ctggcgttaa tgccagcggc cccgctcgtc ctgaaatccg
     2221 tcccggcgat attttccgtg ataactacgg cggcacggta atgattaagg gcgtggagga
     2281 acgtcgcatc acttaccgcc gtgaaggcta cgaatatgac tgcgttatgc ctgtttatca
     2341 gttccggcgt gatttcacgc tggtgggcca tcgtaaagcc gttaatcaga aacgtgctgc
     2401 aagttatatc cgcaaaatcc gtgaaatgct ggtcgcaggg ggtaagaaat gaaactggca
     2461 ccgaacgtaa aactgttacc gaaagataaa cacactgagg cggttatttt tgcgggtgat
     2521 aatgctcact cctttgcaga acattacatg attgcacagg ccaaaaaagc aggcgatccc
     2581 atccctccgg tttacctggg ccgttatcag ttaagcgaac tggacaactt acagattgtt
     2641 gatgaaggac gatacagggc aaaggtcata cgcgccggaa acctggacga tatgcagatg
     2701 ctgaccattg ccacaaaact ggcgatcgcc ggagtccagg aggcgcggct actttctgaa
     2761 aatttcgaac tgctggagga ctggagcgac cagctaccac gactacggga aacatgggag
     2821 agaggggaaa gcctggtcat gaatggcgga aagcgaaaaa tcacacttcc catctcatgg
     2881 ggttctgagg gattcgacgc gcagcaaagc tacgtaatta aggggcttat tccggctgag
     2941 tcattatgca gcacctacgg ggccagcggc tcttataaat cgttcctcgc tatttcctgg
     3001 tcgtgccatg ttgccacagg tatggcatgg gcaggccgca gggtaagtaa gggtgcggtt
     3061 atctatatcg ctggcgaagg cagcatgggc gtaaaacgcc gcgttaaggc gtgggagata
     3121 acccacgaca aggtggttac agatttgtgc atcattaacg cgcctgtttt ccctgcatcg
     3181 cctgactatg tggagcaggt tatcaggact gccggacttg ttaagagcag aacgggcgaa
     3241 aatgtgcggc tgattgtgat cgacacactg gcccgttgct ttggtgggaa tgatgaaaat
     3301 gattcccgtg atatgggcgc atttatccag gggtgtgacg cgataaagca ggccacaggg
     3361 gccacggtgc tggtggttca ccattccggc aaggatgaaa caaagggggc gcgtggttcc
     3421 agtgcattcc gtgccgcgct tgatgcagag taccgaatca gccgggaaaa ttcagacgtt
     3481 acagcactgg tggcggcatg tacgaagatg aaggacgcag aggagccaaa agagagcgca
     3541 tacgacctta aaagcgtgga ggtgtttacc gataccgatg gggaagagat tgtgtctatg
     3601 gtggtgattg acgttccccg cgcccctgct gaactggagc gcatcgagga ggcaggcaac
     3661 aagacggaaa atcataccgc gctatggggg tgcatccgtt cacgtacaca gaacggcgac
     3721 aagtgcacga tcccgctgtt acgtgatgac atgaaacggc ttgggtatga cgtgaaaaat
     3781 atgcggcgat ggttagccaa actggaaaaa gacgccgtga tttacattga tggtgatgat
     3841 gttggcccac tgtaaaaagt gagtggcaaa agtgagtaat ggtgaggttt ttacaaaata
     3901 ttaacacaat tactcacttt ctcacctgta tacatgctca aaagtgagga gcaaaaaaac
     3961 acttatgaaa catgcgcata caaaacacaa aaatctcaag tgagacgaag tgagcaggtt
     4021 taaaaagtga gtagtaaaag tgagagatgg tgagcaatga ataaaaatcg aacagcagga
     4081 caaaataccg aaggtaaatt tatgaacact gaaattgaaa cactgagcat atcaaacgcc
     4141 cttcctggct ggtgggcgaa gttcaaagac gacgacggca cggaatggta tagcccagtt
     4201 gctgcctggg cgctttgtga gatccactat ttcggcaccg gagatactta ccgcgaaatc
     4261 ttacccgtgc tgaccagtga gctgggcatg agtccgcata gtcctgatga gggaatgtgc
     4321 gagtgccttt atctgccgga taagaagttt gttcactgtg gtgaatcgat ggtatttgcc
     4381 tggtatccgg ttaacgacag cagcaatagc ggtactctgg agtaaatcat gaaggtaaac
     4441 cacaaaaaga cagatcaccg cgccattgac cttacagagc actggctgag ggtggcgata
     4501 aaaatcatcg accgcaacac gggggaagga tacgcgaaag cacatcccga actgataagc
     4561 gcattcatga caacgacggc ggcaaacttt gccacgctga cagagcggga tattgccgaa
     4621 gcagaacagg taacaaccat caacgttaaa accggagagc agacagcatg acagcacaga
     4681 tagcagctta cgggcggctg gtggacgacc cgcaggtaaa acagaccagc aagggcacac
     4741 cgatgacgct ggcacgtatg gcggtatcgc tgccatgcag ccaggcacag gacggacagg
     4801 cgacgttatg gctatcggtc atcgcatttg gtaagcaggc cgactttctg gctaaacatc
     4861 aaaaaggcga cgttgccagc gtatccggca cgatgcaggt gagccagtgg accggacaga
     4921 acggggaaac gcggcagggc tggcaggtta tcgcagacag cgtaatcagt gcccgtgctg
     4981 cacgtcctgg cgggagcaga cgcacaacca caggcacaca gggtaatcag ccaccagcgg
     5041 gaggcgatga cccttacggt gatgatattc cgttctgagg gggtgacgat ggtacatgac
     5101 cgcatagcgg aggaactcga ggcaaaagga ttttaccgga gggcggccgc gcgatggggt
     5161 gaagtgatga tgctggtgga gacagacaag gagcgcgaac aggtggcaaa gcgtcgcgcg
     5221 gaatgtatcc gtaaggcagc gcgccaacca gtcataccgg ataatttcgg aatactgaaa
     5281 gaggccataa accgcacaca taccgggatg tgcttacaga aacctggcgg tgagatgttc
     5341 aggaactacc cgaaaaaaag ggataattga taacggttgt taactgtttc tggtatcgtg
     5401 gcagcaggag gaaacatgcc agtgacattt gaagaagtcc agcaacataa aaagtttcat
     5461 ggttttgatg atctggaaac cacgacagca aaaaaatatc gccgtctgct ttcttccgat
     5521 gcgttatttt ttgtggatca taatgatttt ctgcgcagct cactgaccgg ggaaattttc
     5581 gcaaccaacc gtgagcaggt ggaagcgatg atcgaatatc tgtggaaaat aaggcgcaga
     5641 atgcgtgatc ccgtgaaacg gtagaataat aatggcctgg tattttccag gccattattt
     5701 ttagaagtgt tgctgaatga acattgcttt tttcagttca ttttctgaca tagttcgttc
     5761 ctcctgatta acgagcagaa aatgtacgga acagcttatt tattttttca ggttttgtaa
     5821 attatttgtt cgtggttgtt ccaggttgtt cactgagcgg atcggcatat tttacccgaa
     5881 ctgaatcatg attattctcg cccgtggtgc caggacgctg gggccacttt accgcctgtt
     5941 aatgtgctcg ccaatattca ttaccaggcg ggaaaacgat cggtgcgatt gctgatttcc
     6001 ttatgaaaaa cggttgagtg tttgtcgcgt cctggagttc cttacttaac cccaggactt
     6061 ttttttatgc cgagaataat cgaattacgc cagcagaaaa ccgccattaa aaatcagatg
     6121 cgcgacatgc tggagaacgc ggaaaaagaa aaccgcagtc ttaacgatgc tgagggcgca
     6181 aaatttgacg aattacgcgc taaagctgaa tccctcgata aagacatttc ccgccttgaa
     6241 gccattgcag acgaagagcg cagcaagcca ggtaaaagca gtcagaccac tgaccccgca
     6301 gaactacgca actacattct gacaggtgaa acccgcgcat taagtacagg cgttcccgct
     6361 gatggtggtt ataccgttat ccccgaactg aacaccgaaa tcatgcgaat gctggcggat
     6421 gaatccacca tgcgccgcat ctgtaccgtg aagaaaatca gcagcaacga gtttaagcag
     6481 cttgtttccg ctggcggtgc gaccgttaac cacggtgaag agggtaaggc acgcgaacag
     6541 accagcaccc cgaagattaa cgaggtgagc attaagctgt atccggtcta tgcgtacccg
     6601 cgcaccacac aggaaatcgt ggatttttcc gatgtggaca tcctttcatg gctgacgggc
     6661 gagattggcg acaccttcac ggaaaccgaa gaaagcgatc tggttgtggg cgacggtgac
     6721 aaaaaagcaa aaggcttttt atccgtcccc cgtgcagaga agagcgacaa agaacgcgac
     6781 ttcggaacgt tgcaggttat caagcccgct cacggtctgg cgtggacatc tgcggacccg
     6841 ctgatcgacc tgaaatttgc attgcgtaaa aaataccgca agaacgcggt atgggtggtt
     6901 aactccacaa cggcggcaaa acttcagaag gtgaagaacg cgaacggtga ttacatctgg
     6961 cgcgaccgtt tacaggcggg tgatcctgat acgttgctgg gccttccggt cgaatatctg
     7021 gagtttatgc ctgataacgt tattgccctg ggtgacttca aacgcggtta ctacattgtt
     7081 gatcacgaaa caggtgttcg caccagaccg gacaacatca cagagccggg cttcatcaaa
     7141 attttcacgc agaaatattt aggcggtggc gtggtggatt cgaacgcgat caagattctg
     7201 gaactgccac aggacgacga ttaacagcat acagaagggg cttaaaagcc cctttagtgt
     7261 tttatgggtg aaaaaattat gaagagtatg gaaatccggt catcggaaat caccacgagc
     7321 ggagccggta agctgacggg ctacgttgtt cgctgggata agctttcaga actgttatgg
     7381 ggtgagtttt acgaaaaatt ccagcggggg gcgtttactg aatggcttgc ggcgggtaat
     7441 gacgttcgcg gcctgtatga gcatgaccac agcatgttac tggggcgcac ccgttccggc
     7501 acgctgaaac tggaagagga cgaaacaggg ttacgctttg aactgacccc accggatacc
     7561 agtacagggc gcgacgttat cgaactggtt aaacgcggtg atatatccgg catgagcttt
     7621 ggctttcgct cccgtaagga tgtatgggat accacaacag atccatgcgt tcgtaccgtg
     7681 ctggtggcgg aactgtacga aattaccgtt acatcggtac cggcttaccc cgattccggc
     7741 gtggagctgg cccgccgttc cctgtatgag cagcaccccg aaaaaatgcc gcgtgcggat
     7801 aatcgccgct ggtgggtgga tttagcgggg gtgtgatatg tggcttttca gaagaaaaaa
     7861 agagcagcgc agcatgacgc ttgatgaatt tatggcgctg gcgggcacat cgaacacggg
     7921 ggcgggtgag tacgtatcat cggggacagc ggaatcactg cccgccgtca tgaacgccgt
     7981 gacggtcatc tctgaggcgg tggctaccat gccgtgttac ctgtacctgg tacgcaatga
     8041 gaaggggaag gaggcccgcg agtggcttga ttctcatccg gtcgatcaca tcctcaacga
     8101 gcgcccgaac gcatggcaaa ccccctacca gtttaagcga atgatgatcc gccactgcct
     8161 gttaaacggt aatgcttatg cggtgattca gtgggggcgt gatggttttc cggtggcttt
     8221 acatccttac ccgccgcagt cggtgaacgt ggagcagaca ggtgagcata actggcgcta
     8281 ctgcatcact gacgcctaca ccggaaacac ccgcaactat ttaccgtggg aggttctcca
     8341 ccttcgttac tccacggatg acggctttat ggggcgctca cctgtaacca tctgccgcga
     8401 atcgctgggg cttgggctgg cccaacaacg ccacggcgcg agcgtgatgc gtgatggcat
     8461 gatggcggca ggggttatca cgtcaggcga atggctggac ggcgtgaagg gcaaacaggc
     8521 attagccgca ctggaacgct acaaaggggc cagaaacgcc ggaaaaacgc ccatccttga
     8581 agggggtatg agctatcagc agctgggcat gagtaatcag gatgctgaat ggctggcctc
     8641 ccgtcgcttt accattgaag acatcgcccg aatgttcaac gtctcgccga tttttttgca
     8701 ggaatacagc aacagcacct acagcaattt cagtgaggca agccgcgcgt ttctgaccat
     8761 gacaatgcgc ccgtggctgg cgaacttcga acagcaaatc aaggctgctt tgctggtggc
     8821 ttctcccgta cctggtaccc gttatctggt tgagtttgat tcagccgatt tattacgcgc
     8881 cacccctacc gaacgttacg ccacgtatga gaaagggatt aagaacggga tcatcaatcc
     8941 gaacgaagcc cgtgagcgtg agggaatgcc gccgcgtgaa ggtggcgacg agttcagcca
     9001 ggcatggaag caggaagtga caatcagcaa gaacagtaag ggcggtgacg aatgatagcc
     9061 ggaaagatgc gggaccgggt gacgattatg acatttacca cacagcgcct gccgtcaggt
     9121 gcgatccagc agatatggac ggaaggggaa accatctggg cagaggtcaa ggggatcagc
     9181 ggacgggaat tactgaccgc aggcgcagaa accgcacctg caacgattcg ggtatgggtt
     9241 cgctatcgtg gcgacatatc cgccgccagt cgcctgaaag tgcttaccgg cgcttttgcc
     9301 gggagcacgc ttaatatcat cgggatcccc ttacctgatg cgaaaagaac ccggctggaa
     9361 attctggcaa aggtgggtac agaaaaatga cagcagaaaa aataaccctg gaagaggcca
     9421 aacttcactg ccgcatagat ggtaacacag aagacacact gattcaggca tacataagcg
     9481 cggcgctgga ggtatgccag aagcatattg gcaggcgttt tgatgacggc ctggaattta
     9541 cccctgcaat aaaaattggt tgcctgatgt acatctcgca actgtacgag taccggacaa
     9601 cgcttggcga taccgataca aaagaaattc ccctgacgat ttccgcgctc tggtcggttt
     9661 atcgtgatgt gggggtgtac tgatgccgtg gcaaccattg agacgatgca cacaaccagg
     9721 ctgcaacagg cgcgtgaagt ccgggaagtg tgaggagcac agacgcagcg cacgccagca
     9781 gcaggacagc cggagaggca gcagcaggga gcgagggtat acgaggcagt gggagaaata
     9841 ccgcgctatg tacctgagta aaaacccgtt atgtgcgcat tgcctggaaa aaggaatata
     9901 cacgcccgcc gtggtggtgg atcacattat ccccatcgat ggcggtaatg atgttctctt
     9961 ctggcctgag tggaatcacc aggcattgtg ccagacgtgc cacaaccgta agacgacacg
    10021 ggaagatcca gccacgaaag caaaccgtaa agcgggcatg tatcgcgagc aggaagaacg
    10081 ggcggcacac cgtaacgact ggatgtatgg cgatgatgac tgaacaggag caaaccaggc
    10141 tgatacgtgg actgataagg cagcgtgaca catggaagac acaggagaca gagcacaaag
    10201 ccaacaggac agggcgcaca aaacgcacca cagcgaagcg attaaccgac cgtgaccgcg
    10261 aggtcatgga atgttttcgc aatcgctgat gaggcagccg gacggggtgg ggggggagtt
    10321 ttcaggacga acccgaccct gcccagaacc gaccgcctgc tcaaattttt gtgcacggga
    10381 atttttttga aaataattgg gcgaaaaaag aacatggcaa gaccaccaaa agcccccgct
    10441 tacctggatg aaatcgcggt caggcagtgg aaggaaaaat cgcgccagct ttccggacgg
    10501 gaagacctta ctcccgccga ctggagcaat ctggagctgt attgcgttaa ctactccata
    10561 taccgcaaag ccgtcgaaga ccttgccacg cgcgggttca gcattgttaa cagtcagggc
    10621 agcgagagca gaaaccccgc cctgagcgca aaggctgacg cggaaagaat aatgatcaaa
    10681 atggcttctt tgctgggttt tgacccggta agccgccgca gaaatccacc ggaaacagag
    10741 gaagaggacg agcttgaccg cctggcatga gtacgcagaa ggcgtaaaaa acggcaaaat
    10801 tacggcctgt aaacgactga aacaggctgt taaacggtat ttttctgacc ttgaaaaccc
    10861 cctttacacg ttcgatccgg aggtcgtgga gcggtttatt gccttttcca gggtgtgtcc
    10921 gcacgtaaaa ggcgcaatgc gcggtagccc cattgagctg gagccgtggc agcagttcgc
    10981 ctttgcgtgc atcctgggct ttaaggttaa ggccaccgga cggcgcaaat acaccagcgc
    11041 attcattgaa gtaccgcgaa aaaatgccaa atccacggtc gccgctatcc tggctaactg
    11101 gtttctggtt atggaaaacg ggcagcagga tatttacacc gccgccgtga gtcgtgatca
    11161 ggcgcggatc gtgtttgatg atgcgcgtca gatgtgcctt ttatcccgac cgttacgaaa
    11221 gcgggtaaat attcaggcac acaaggtgat acacgcgaaa accaacagcc tgttaaagcc
    11281 actggcagca aaagcggcaa ccattgaagg tacaaacccg agtcttgcca ttgtggatga
    11341 atatcacctg caccctgaca acggggttta ttccgcgctt gagctgggaa tgggggcgcg
    11401 tccggagggg ctgttatttg ccatcaccac atcggggagc aacgttgttt cagcctgtaa
    11461 acaacactat gactattgct gccagatact ggatggtgaa gaggtgaacg aatccatgtt
    11521 cgtgctgatt tacgagctgg atgatgaaag cgaggttgac gatccggcga tgtggataaa
    11581 ggcgaatccc aatatcgatg tttccgtcga tcgtgaaaaa ctggcctcaa ccatccagaa
    11641 agcgcggggt attccgtcgc agtgggtgga aatgctcacc aagcgattca atatctggtg
    11701 tcagggggct acgccgtgga tgggtaacgg tgcatgggcg gagtgcgccg gaacgttcgc
    11761 ggaggcggat ttatacgggc aggagtgcta cgcggggctg gacttatcat caaccagcga
    11821 tatttccagc gtgtgctatg ccttcccggt cggtaaaaaa attatgctgg tttcccgtca
    11881 ctatctgccg gaatttcagc tacagaaccc cgccaataaa aaccgcgcca tctatcgcca
    11941 gtgggtaaag gctggctgga tacgcacaac accgggtgac tgcattgatt atgaccgtat
    12001 ccgtgatgac atcatggcgg atgcagagaa tttcagtatc aggctggtgg gcttcgatac
    12061 atggaacgcc actcacctga gaacacagct acagggggcg ggatttgagg tggagccgtt
    12121 cccgcaaaca taccttcgtt tcagtccggc ggcgaaatcg ttcgaagttt ttgttaaccg
    12181 gaaggtgatt gtgcatcgtg gtgatccggt gctggcctgg tcaatgagta atgttgtgat
    12241 gcagagtgac gcgaacgcca atatcaagcc gaacaagaaa aaatcatcca acaagataga
    12301 cccgagcgtt gcggcgctga tggcgtttgg cacattccag gcagagcatg aggaatttgc
    12361 attcgatatg agcgacagcc acaaagagcg gcttgcggcg tttgatgggg tatgacgaga
    12421 atgagcgaag ccgaactact gggattaatc cgccgcgtta ccggaatcag ccagcagcat
    12481 gacgaacagg ccacgcagcc ggacagcgtg accgccgaaa attatgtgcg tgttgttgcg
    12541 gaggtgatgc gccgtgatgg tattgagctt aacggcgtgg atatgcgcga catacgaatc
    12601 agagtactag agttgctggc ataccgtcga cgcgtggcga tgtatcagga gagcgcgaaa
    12661 aacacttatc agtggaagaa gccggaacgg ttgcggcggt aaccagttga taaaaagggt
    12721 cctcaatttt gaggagggta gctggtggct ggcagaatgt aaatttacat tctggtgatt
    12781 taatcatctg atttacccga tggcttacct taccaggcca tagcggatta ccgtgcaatg
    12841 cgcaccatcg ccaccaatac gcacatcaaa acgtggctca cggactttca cacgctccag
    12901 caacttatca cgaaccatag cgtttagcgt ccttcgtgtt gcctctatgt gatatcgccg
    12961 atattcggaa ccatagagca gcactgtcac accacgaaca tcaaaaggcg gatctccggc
    13021 ctccacccgc acccaatcgc gatactcagg tttgtagagt tcaagaatct gttttttgtg
    13081 cctagtcatt ctcatgcgtg caactcctta ttgtgggaca gtactgtcat acaataagct
    13141 atgcgggaaa ttatcaaaag aacagaaacc aaccattcag agttaacagg tatcaatggg
    13201 attatgctta tggtgcatga tgacaaagcg gaagaactgg aagcgaaagg tttatggcga
    13261 cgggcggcta atcgctgggg tgagttactg aaacaggcgg caagtgatga agcgcgtcag
    13321 tatgtaacgg aacgacgaac ggcatgcatc cggaaggcaa ccataacccg ggaaccggaa
    13381 agagacctga tttgtgccat aagaaccgcc gccacaaaaa cactaacgga catggggatc
    13441 gacctgaaaa aagaagatcc actgcggggc attcagcacg ggcatacaaa gcaggataaa
    13501 cgcaggaagt aaattttttc gtgtaaccag gcaaccgact atctgaaaga tagtcacccc
    13561 gtaccaggat cacgcaaata acgcacaaca gagtgaccca tagccaccct gttcaaccgt
    13621 tccggctgac tggacagcat catcaccatg atggtgacgc agcccaccag caaccataac
    13681 gatttccttt acagttgatg tgcttttttg ctgataaggc gtaacccgtt gattgttcca
    13741 gaatccccca ttggtggaag ctggataact cacccgccag attctgaatg gtgaaaatcg
    13801 ccgagtagct gaatcaccat ttcgatgatg caggtcgcac cgttttcggg agaaggtgcg
    13861 attatgttcg tcatacctgt tccgttgaaa cggaataccc cattccggcg tagctggtgg
    13921 acgcacagcc acgaaattaa aaaaatgcgt tacgctggtg ggcatgatgc cggattaggt
    13981 gcggacgtaa tacgtacgga catcgagaca aaaacgaaca gtattctggt tcagtgcaaa
    14041 acatcgttaa acgtggtttt gcgtccagag tcatcgcaat ggttttatgc gtcctgcgtt
    14101 gcgctggtgg gcttttccac cattggcgga aaacatggca gcacatcaac cagccacatc
    14161 aaacatcaac agaacggggg gtaaatcaca tgctgccgca gtattcgcta tcagggcggc
    14221 aactccacag gaaatgatat acaggccgtc agaaggctta aaattcgatg aggcagaaaa
    14281 ccggataccc gcactatccg aaaagtgtag tgccccaagt agatcattca ccagccgaat
    14341 cagcacagaa aggggatagc ctgatacaat gttcccccct tcctagtaaa ctcatctgcc
    14401 acgagtagaa agagcgatcg gacccgataa gcagaaatgt ttgtcgggtt cagcttttta
    14461 aggcgctgta gatgcctggt tatccgtgat catccaccag cttcatgacg gatagctgaa
    14521 aaatgataac ggtatagcct gccgcttgtg tctatttgtt ccacgttgtt tcatacggtg
    14581 cgccgaacgg tgtagttaat ggtgtagtta atttgcgatt tttggcactt tttgtggtgt
    14641 aatttattta attaaatcaa tatgttattg aaaataagtt atagatatat cccatttatg
    14701 actccttatt tctgccaggc aagttgataa agcatgagtg ccaacgtttt gaccccttcg
    14761 gcaaggtcgg taatattggt gcgttccgcc gggttatggc tgatcccgtt gatgctgggg
    14821 ataaaaatca tgcaggttgg tacgcgaggc gcgaaaattt gcgcgtcgtg cccggcacca
    14881 ctgtgcatca cccggtaatt cagtttttct ctttcacaca attctgtcag ggtggcgacc
    14941 agctccttat tcatcggcac gggttcttcg tccatccata aatcgatatc aataccaatg
    15001 tccatttcat cgcaaatcgc ccgcatgtcg ttttctaact gttgggtgaa atcgcgcagc
    15061 acggcagcgt cggtatgacg acaatcaatg gtgaacgtgg ttttacccgg caccacattt
    15121 accgtattcg ggcgcggctc tactttgcca aaggtcagaa ccagcggatc gcccatcctt
    15181 ttcgcttttt cgaccgactg atggcaaatg cgactgaaag cgtaaactgt atcacgacga
    15241 taacccatcg gcgtggtgcc tgcatggttt gattcgccgt tcagcgttac cgtataacga
    15301 cgctgcccga caattgcatt caccacgcca attgattgcc cattactttc cagcacacag
    15361 ccctgttcaa tatgcagttc aacaaaggct ttaatatcct gacgcggagt tagtggggcg
    15421 ttcggaagag taaatccgca agccttcatc gcatcgacaa aactatttcc tttggcatca
    15481 cagatattcc gcacgtcgtc aggattcgcc agcccaaaaa tatttttact gccccagaag
    15541 acatacggga agcggctgcc ttcttcttct gccatcgcca ccacttcgac cgtacgtagc
    15601 ggcgcgccgt attgcgtttt cagccagtca attgccagcc acgccgccag cgcgccgaat
    15661 tgcccgtcaa ggttaccgcc gttaaccacg gtatcgatat gcgaaccgct cagaaccact
    15721 tcctgtggat attcggtgcc attcaggcga ccgtataaat tccccacttc atcgaaacgt
    15781 gtttccagcc cgcttgctgc cattcttttt ttaaattgct gctgggtttc cagccattcc
    15841 ggcgaataaa gtaaacgggt catcccaccc gctgggtcag cgccaaaaga ggaaagccag
    15901 ggcagcgttt cttctatagc ttgacggaaa tgtgtaatca taagaaagtc ctgtctcaat
    15961 aattattgcg caaagggatt tttcgtttcg tatgacgtgt tataaagcgc gtcggaaatt
    16021 aaatactggt aaatatcatc aataatttcg atgccttcga cggcggcttt gcgttgttta
    16081 atatcctgat cctgtccggg ataataaacc tgattaaaac cgggcgcggg ggtaatggca
    16141 tttaattcgc gcatggtctg gctaagatgt tgacggaata attcgctgga ggagaaaaag
    16201 ttcggattaa taactatatg taattgcccc aaattacgcc ctgcgtgtaa atcgtcatac
    16261 atcgaactaa cctgtcgccc gaacggtaag ccgagtaaga cgcctgagag gacgtcaatc
    16321 atcatcatca ggccataccc ttttggcccg gcggcgggga gcagagcatg taccgcgaac
    16381 ggatcggttg ttggtacacc gtttttatcg accgcccagg tatccgggat agacatatta
    16441 cgcgagcggg cgtcgagcac ttttccccat gcctgtacgg tagtcgccat atcaaaggta
    16501 aggatctcgt cgccttctcc cggcgcggca aaggccaggg ggttagtacc gtagtaaatt
    16561 tccgcgccgc caaacggcac caccattgga tcggactggc acatcgaaat gccaatgaat
    16621 ccggcgcggg ctgcctgctg cacaaaataa gagattgcgc cgctgtgacc catccggctg
    16681 ataccgacca ccgcaacgcc attttgctgg gcggttttga tggcatgttc catacccatt
    16741 ttcgccgcga cctgtccggc ggcattgtcg gcatgtaaaa ttgccgagca cggcccggtt
    16801 tcctcaagac gaaactccgg ttcgcggttg gtgccgcctt ttgaaatgcg ttccgcgtag
    16861 tattccacgc gcaccgcgcc atgagagtgg atccctctgg catcggcgta aaccaatact
    16921 tcagccacgg ttgcagcgtg ctcacgtttt aacccagcct ggcagagttt attctcaatt
    16981 agctggtgga gtgtttcccg actgattttc atctgtcttc ctttttaacg acggtgtgaa
    17041 gcatgactgc aattaacata cagggaaaat atctggatta tgtgatccag acaggcaaga
    17101 aaatatagtt agaatttatt tgataatccg ctcactttta acctgatttt taaaacaacc
    17161 acgcttatta aaaaataatg agtaatagcc tggcggttat ttgaattctt ttgttaataa
    17221 ttcctgtgtg atattcatca ccttatttac tcgttgtcat cgataccgta atcgccacat
    17281 taacactgct cgtgcaattg ccatgggtgc aatttttaag gagttgttat gatccacgcc
    17341 tttattaaaa aagggtgttt tcaggattcg gtcagtttaa tgattatttc acgaaaactc
    17401 agcgaatcag aaaatgttga tgatgtttcc gtaatgatgg gtacgcccgc caataaagcg
    17461 ttattagata ccacaggttt ctggcatgac gattttaata acgccacgcc gaacgatatt
    17521 tgcgtggcaa ttcgtagcga agcggcggat gcggggatcg cgcaggcgat tatgcagcag
    17581 cttgaagagg cgctaaaaca actggcgcag gggtcaggca gcagccaggc gttgacgcag
    17641 gtgcgtcgct gggacagtgc ctgtcagaaa ttacccgacg ccagtctggc gctgatttca
    17701 gtggctggcg agtatgcggc ggagctggcg aaccaggcgc tggatcgcaa cctcaacgtg
    17761 atgatgttct ccgataacgt cacgctggaa gatgaaatcc aacttaaaag ccgcgcgcgg
    17821 gaaaaaggct tgctggtgat ggggccggac tgcggtacgt cgatgattgc cggcacgccg
    17881 ctggcttttg ctaacgtgat gccggaaggc aatattggcg tcattggcgc ttccggtacc
    17941 gggattcagg aactgtgttc gcagattgcg ctggcagggg agggaattac tcacgcgatt
    18001 ggccttggcg ggcgcgacct cagccgtgaa gtgggcggca tcagtgcgct gacggcgctg
    18061 gaaatgctca gtgcagacga gaaaagcgaa gtgctggcat ttgtttcaaa accgcctgcc
    18121 gaagctgtgc gtctgaaaat tgttaatgcc atgaaagcaa ccggcaaacc gacggtggcg
    18181 ctgtttttag gttatacccc ggcggtggcc cgcgacgaga atgtctggtt tgcctcctcg
    18241 ctggatgagg ccgcacgcct ggcttgcctg ctttcacgcg ttacggcgcg acgtaacgcg
    18301 ataatgcctg ccagcagcgg atttatttgc ggtttgtata ccggcggtac cctggctgcc
    18361 gaagcggcgg gattacttgc tggacacctt ggcgtggaag ccgacgatac ccatcatcac
    18421 ggcatgatgc tcgacgccga tggtcaccag attattgacc tcggcgatga tttctacacc
    18481 gtcgggcgtc cccatccgat gatcgaccca gccttacgca atcagttaat tgccgatctc
    18541 ggcgctaaac cgcaagtgcg cgtattgctg cttgatgtcg tgattggctt cggtgcgacc
    18601 gccgatcctg ccgcctcgct ggtaagcgcc tggcaaaaag cctgtgctgc gcgttcagac
    18661 aatcaaccac tgtatgccat tgccacggtg acaggcactg aacgtgaccc gcaatgccgc
    18721 tcgcagcaaa tcgccacgct ggaagatgcg gggatcgcgg tagtgagttc gctgccggaa
    18781 gccaccttgc tggcggcggc actgattcgt ccgctttcgc ctgccactca gcaacacaca
    18841 ccgtcattac tggaaaacgt cgccgtgatt aacatcggat tacgcagctt tgcgctggag
    18901 ctacaaagcg ccagcaaacc ggttgtgcat taccaatggt cgccagtcgc cggcggcaat
    18961 aaaaaactgg ctcgtttatt agaacgtttg caataagggg ttcccatgtt tacatcagtg
    19021 gcgcaagcca atgctgcggt tatcgaacaa attcgtcgcg ctcgtccaca ctggctggat
    19081 gtgcaaccgg cttcttcact tatcagcgaa ctaaaccagg gcaaaacact gcttcacgcc
    19141 gggccgccaa tgcgctggca ggagatgacc ggacctatga aaggggcgtg cgtgggcgca
    19201 tgtctgttcg aaggttgggc gaaagatgaa gcgcaggcgc tggcaatact ggagcagggg
    19261 gaagtgaact tcattccttg tcaccatgtg aatgccgtcg ggccaatggg cggtattact
    19321 tctgccagta tgccgatgct ggtggtcgag aacgtgaccg acggcaaccg ggcgtactgc
    19381 aacctcaacg aaggtatcgg caaagtgatg cgttttggcg cttatggcga agatgtcctg
    19441 actcgccatc gctggatgcg cgatgtgtta atgccagtat taagcgcggc gctggggcgc
    19501 atggagcacg gtatcgatct cacggcgatg atggcgcagg gcattaccat gggcgatgag
    19561 ttccatcaac gcaatattgc ttcctcagca ctgttaatgc gtacgctggc cccacaaatt
    19621 gctcgcctcg atcatgataa acagcacatt gcggaagtga tggatttcct cagcgtgacc
    19681 gatcagttct tcctcaacct cgcgatggcc tactgcaagg cggcgatgga tgctggcgcg
    19741 atgatccgcg caggcagcat cgttacggct atgacccgca acggcaatat gttcgggatt
    19801 cgggtaagcg ggctgggtga acgctggttt actgcgcctg taaacactcc gcaaggtctg
    19861 tttttcaccg gcttctcgca ggagcaggcg aacccggata tgggcgatag cgcgattacc
    19921 gaaacctttg gtatcggagg tgcggcaatg atcgcagcgc ctggcgtaac gcgctttgtc
    19981 ggtgcgggtg gcatggaagc ggccagggcg gtatctgaag agatggcgga aatttacctt
    20041 gaacgcaata tgcagttgca gatcccaagc tgggattttc agggcgcctg cctgggactg
    20101 gacattcgtc gcgtggtaga aaccggtatt acgccactca tcaataccgg tatcgcccat
    20161 aaagaggcgg ggatcgggca gattggcgca ggcaccgtgc gggtaccgct ggcgtgtttt
    20221 gaacaggcgc tggaagcact ggctgaaagc atggggattg gttgaggaac gcacaatgac
    20281 gatcatccat cctctgcttg ccagtagtag cgcaccgaat tatcgccagt cctggcggtt
    20341 agcgggagtg tggcgacggg cgattaacct gatgacggaa agcggcgaac tgttaacgct
    20401 gcatcgtaag ggtagtggtt tcggccccgg aggatgggtg cttcgccgcg cgcaattcga
    20461 tgcgttatgc ggtggattat gcggcaatga acgaccgcag gttgtggctc aagggattcg
    20521 cctcgggcgt ttcacggtta aacagccaca gcgttattgt ttgctgcgta ttacgccgcc
    20581 tgcgcatcct caaccgcttg cagctgcatg gatgcaacgc gcggaggaaa ccgggctttt
    20641 cgggccactg gcgatggcgg caagcgatcc gctgcccgct gaattacgcc agtttcgtca
    20701 ctgttttcag gccgcgctca atggcgttaa gaccgactgg cggcactggc tgggtaaagg
    20761 acccggatta acgccaagtc atgatgatac gctgagcgga atgctgctgg cggcctggta
    20821 ttatggcgct ttagatgcgc gctctggtcg ccagtttttt gcctgttccg acaatcttca
    20881 actcgttacc acagcggtga gcgtcagtta tttacgctac gccgcgcaag gatatttcgc
    20941 ctcgccactc ctgcactttg ttcatgctct gagttgcccg aaacgtaccg ctgttgcgat
    21001 tgattcgctg ctggcgctgg ggcacacgtc aggggcagat acgctgctgg ggttctggct
    21061 tggtcaacaa ttattacaag gaacaccatg aaaacactgg ttgtagctct tgggggcaac
    21121 gccttactcc agcgcggcga ggcgctgacg gcagaaaatc aatatcgcaa tatcgccagt
    21181 gctgtacccg cgctggcgcg cctggcccgt tcttatcggc tggcgattgt tcacggcaac
    21241 gggccgcagg tggggctgct ggcattgcag aatctggcgt ggaaagaggt agaaccgtat
    21301 ccgctggatg tgctggtggc ggaaagccag gggatgattg gctatatgct ggcacagagt
    21361 ttgagcgcac agccgcagat gccgcccgtg acgacggtgc tgacgcgcat tgaggtttcg
    21421 cctgatgatc cggcgttttt gcagccagag aaatttattg gtccggttta tcagccagaa
    21481 gaacaagagg cactggaagc ggcttacggc tggcagatga aacgtgatgg taaatatttg
    21541 cgccgggtgg tggcgtctcc gcaaccgcgt aaaattctcg acagcgaagc catcgagttg
    21601 ttgctcaaag aggggcatgt ggtgatttgc agtggcggcg gcggtgtgcc tgtgacggaa
    21661 gatggggcag ggagtgaagc ggtgattgat aaagatctcg ctgctgcgtt gctcgctgag
    21721 cagattaacg ccgatgggct ggtgatcctc actgatgctg atgcggtata tgaaaactgg
    21781 ggaacgccgc agcaacgcgc cattcgccat gccacaccgg atgagttagc gccatttgcc
    21841 aaagccgatg gttcgatggg gccgaaggta acggcggtga gtggttatgt cagaagccgt
    21901 ggtaaacccg cgtggattgg ggcgttatcg cgaattgaag agacgctggc gggcgaagcg
    21961 gggacctgta tttcgctgta gtcgtaggca ttagacattt gtgcctgatg cgacgcttga
    22021 cgcgtcttat caggcctaca accggtgccg catccggcaa ttggtgcaca atgcctgatg
    22081 cgatgcttga cgcatcttat caggcctaca atgggtaccg gatcggtagg ccggataagg
    22141 cgtttacgcc gcatccggca agaatagagc accagttaac cgaacttact ctgcgcccaa
    22201 atcacgccgc tggcatattc cggcggcagc agcgggatta aggcttccag cgtcgcagtc
    22261 agacgcgatg tgtcgctgtc ggtcaaattc agatgcccca ctttacgccc cggacggact
    22321 tctttgtcgt accagtgcag atgcaccagc ggcaatttca gccagtcata attcacatcg
    22381 ctaccaatca gattgatcat caccgacgga ttattcacta ctggttgcgg taacggcaga
    22441 tcggtaatcg cccgcagatg cagctcaaac tggctgatgc tggcaccgtt ttgcgtccag
    22501 tgaccactgt tatgcacacg cggagccagt tcgttgatca gcagaccttg cggggtgaca
    22561 aaacactcca tcgccatcac gcccacatag cccagctcct gcataatcgc cgacagcatc
    22621 tcttcggctt gcgcctgctg ctgtgcgttg gcctgcggaa aagcgacgct ggtgcgcaaa
    22681 ataccgtcct gatgcaggtt atgcgtcagc ggataaaaca cggtgctgcc atcaaagccg
    22741 cgcgcgccaa ccagcgacac ttcaccagag aagtttatgc cctgctcgac aatacattcg
    22801 ccgtagcatt cagccggtaa ctgttcggtt tcatcggcgc gtaaacgcca ttgaccgcgt
    22861 ccgtcatagc caccagtgcg acgcttaaca atcgccagtt cacctaaacg atcaaacacc
    22921 gcaggccact cgctgcgatc ggcaagtaac tgccacggcg cggtcggcag gtggagctta
    22981 tcgaaaagct gcttctgagt cagacggtcg gcaataatcg ggaacacatc gcggttcaca
    23041 aaggccggat ggcgtgccag ctcgcgggtt aatgcggttt ccggccaacg ttcgatctca
    23101 gcggtaatca cgctttgttg aaaaggcacc gccgccggtt cagcgtccag cccgaccggc
    23161 cagacagcaa tgcctaacgg ttcgcctgcc tgacgcagca tacggcctaa ctgcccgtta
    23221 ccgaggacgc aaacctgttt catgccgcac ctcgcgggtc cgggttttcc agcacttcat
    23281 cagtttgtgc tttgcgccag tcattcagac gttggtacag ttctttatca tgagtcgcaa
    23341 gaatttgtgc tgccagtaat gccgcgtttg ccgcgccagc tttaccaatc gccagcgtac
    23401 ccaccggaat gccgcgcggc atttgtacga ttgagtagag gctatcgaca ccgctcagtg
    23461 cggcgctctg tactggtacg cccagcaccg gcaccagcgt tttggcggca atcatgcctg
    23521 gcaggtgcgc tgcgccgcct gcgcccgcaa taatcacctg ataaccgttc tcttcggcgc
    23581 tttcggcgaa gctgaacagt ttatcggggg tgcggtgagc agaaaccact tcaacgtggt
    23641 gcgggacatt caggatttcg aagatttcgg cggcgaactg catggtagcc cagtcgcttt
    23701 tggaccccat cacgatggcg acacgcgccg gattattgcg ggaagacatg cgtcttaaaa
    23761 ctcctgtggt gcacaactct cggctttaga gggcacagag aatagcacgg aaagagagta
    23821 aggaaaacgg ttgcgtggct gtgaaatcag caaagttgcg ggttttttaa aacggaaaat
    23881 gaatcagctc aacgtcatcc gccgtgactt tcaccatcga accttccgta tgccaggcac
    23941 ccagtaccac gcgaaaagca ggttgctggt tggcgataag ttcatgtacc gccgggcgat
    24001 gggtatgccc gtgaatcagc cattgcacct gatgtttttc catcgcactg accaccgcgt
    24061 tttggttaac gtccatgatc gccagcgatt tactgctgtt ggcttctttg ctgttcgcgc
    24121 gcattcgcgc ggcaatgcgt ttgcgcacaa acaatggcag agcgaggaat agcgtctgca
    24181 gccagggttt gtggactttg gcgcgaaaag cctgataacc cgcgtcatcg gtgcacagcg
    24241 tgtcgccatg cataatcaac acccgacgac cataaagttc gagcaccttt tcttccggca
    24301 ataacgtcat gccgctttca cgggcaaagc gtttgccgag cagaaaatca cggttgccat
    24361 gaatgaaata acagggaacg ccggaatcgg acaccgcttt gatcgctgcc gccatctggc
    24421 gatggagtgg gttgggatcg tcgtcgccaa tccacgcttc aaacagatcg ccaagaatat
    24481 acagcgcgtc ggccttgcga gcttcccccg ctaaaaaacg cagaaaaccg gcggtgatcg
    24541 ccggttcttc cacgcagaga tgaagatctg caataaagag tgtcgccacg attactcgct
    24601 aacggtcacg ctttcaatga taacgtcttc ttttggcacg tcctggtgca taccgctacg
    24661 accggttgca acacctttga ttttgtctac cacgtccatg ccttcaacca cttcagcaaa
    24721 cacgcagtag ccccaacctt gcaggctttc gccagagaag ttcaggaagt cgttatcaac
    24781 cacgttgatg aagaactgtg cagttgcaga gtgcggagcc tgagtacggg ccattgccag
    24841 cgtaccacgg gtatttttca gaccgttgtt ggcttcgttt ttgatcggtt ctttggtggc
    24901 tttttgtttc atgcccggtt caaaaccgcc gccctgaatc ataaagccgt tgataacacg
    24961 gtggaaaatg gtgttgttgt aaaaaccttc gcggcagtag tccaggaagt ttttaactgt
    25021 ttcaggtgct ttatcgtcaa aagttttgat gacaatatcg ccgtgattgg tgtggaaagt
    25081 aaccattttt gcatcctgtt ccgtttgatt ggtgcttcaa cccggttcgg gtcatatata
    25141 gggtggtgtt atagcataac cgcacgatcg gatcatcacg caatgtatgc tgattcgcgc
    25201 gggaaatatg ggtattatac gcaactcaat tacccacaca tgtctaaacg gaatcttcga
    25261 tgctaaaaat cttcaatact ctgacacgcc aaaaagagga atttaagcct attcacgccg
    25321 gggaagtcgg catgtacgtg tgtggaatca ccgtttacga tctctgtcat atcggtcacg
    25381 ggcgtacctt tgttgctttt gacgtggttg cgcgctatct gcgtttcctc ggctataagc
    25441 tgaagtatgt gcgcaacatt accgatatcg acgacaaaat catcaaacgc gccaatgaaa
    25501 atggcgaaag ctttgtagcg ctggtggatc gcatgatcgc cgaaatgcac aaagattttg
    25561 atgcgttgaa cattctgcgc ccggatatgg agccgcgcgc gacgcaccat atcgcagaaa
    25621 ttattgaact cactgaacaa ctgatcgcca aaggtcacgc ttatgtggcg gacaacggcg
    25681 acgtgatgtt cgacgtcccg accgatccga catatggcgt gctgtcgcgt caggatctcg
    25741 accagctgca ggcaggcgcg cgcgttgacg tggtcgacga caaacgcaac ccgatggact
    25801 tcgttctgtg gaagatgtcg aaagagggcg aaccgagctg gccgtctccg tggggcgcgg
    25861 gccgtccagg ctggcacatt gaatgttcgg caatgaactg caagcagctg ggtaaccact
    25921 ttgatatcca cggcggcggt tcagacctga tgttcccgca ccacgagaac gaaatcgcgc
    25981 agtccacctg tgcccatgat ggtcagtatg ttaactactg gatgcactcc ggaatggtga
    26041 tggttgaccg cgagaagatg tccaaatcgc tgggtaactt ctttaccgtg cgcgatgtgc
    26101 tgaaatacta cgatgctgaa accgtgcgtt acttcctgat gtcgggccac tatcgcagcc
    26161 agctgaacta cagcgaagag aacctgaagc aggcgcgtgc ggcgctggag cgtctctaca
    26221 ctgcgctgcg cggcaccgat aaaaccgttg cgcctgccgg tggcgaagcg tttgaagcgc
    26281 gctttattga agcgatggac gacgatttca acaccccgga agcctattcc gtgctgtttg
    26341 atatggcgcg tgaagtgaac cgtctgaaag cagaagatat ggcagcggcg aatgcaatgg
    26401 catctcacct gcgtaaactt tccgctgtat tgggcctgct ggagcaagaa ccggaagcgt
    26461 tcctgcaaag cggcgcgcag gcagacggca gcgaagtggc tgagattgaa gcgttaattc
    26521 aacagcgtct ggatgcccgt aaagcgaaag actgggcggc ggcagatgcg gcgcgtgacc
    26581 gtcttaacga gatggggatc gtgctggaag atggcccgca agggaccacc tggcgtcgta
    26641 agtaattgcg ctattgccgg atgcgagttt tcgcatccgg ttatcgtctg tgccaccaca
    26701 acattcccat cagtagaatc cccggcaacc acacccacat caattcagaa ataatcacct
    26761 gatgcccgta cggcgtggtg taacgagaca atgcaaacgg cgcgacttta atcacctgcc
    26821 agggagcgaa aaagcgttca tctgaccacg gccacagcca gccaacccct ttaccgccag
    26881 tggttaccga atccagcaag ctgtgcgata gcaacgagac ggttaaaaac agccagcagc
    26941 gaatcagccc agccctgaac catcggcgtc caatcagcac acataacagc gggacaacaa
    27001 acgcaaacac cagcgaatgg gtaaacccgc gatgaccaaa aacattgccg taagcaatgc
    27061 caaatttaaa cgacaatacg tcggcgtcgg gcagcatcgc caggatgatt ccggcaaata
    27121 acagacgcgg agggatgact ttcgaaccca accctaaacc aatgcatagg ggaacggcgg
    27181 cgtgcgtaat aacggttggc atgatggtcg cttcggcaaa atgtcgatgc tatcagcatg
    27241 gatgaacggg gcgtagaggg caaaagtctg aaaagagaac cggcctgttg atacaggccg
    27301 ggaaagggat caggcaacaa cctgtacgct gtgacctgca aaactcactg tctgaccggc
    27361 gacgattttg cagcgtttgc gcgtttcaac cgcgccatcg actttcacct ggccttcggc
    27421 aatcgcgatt ttcgcctgcg cgccgctttc gctccagcct tccagtttca gcaagtcgca
    27481 cagctcaacg tgcggatgtt tacctaaaga aaatgtcgcc atgttactca ccctgtggat
    27541 catgatattc aacgcacgcc tgtagcgtgt tttcaatcag cgtggcaacc gtcatcgggc
    27601 caacgccgcc gggaacaggc gtaatgtatg aggcgcgttt agccgcgtct tcaaacacca
    27661 cgtcgcccac aactttgcca ttttccagac ggttgatgcc gacatcaatc acaattgcgc
    27721 cttctttgat ccagtcacca ggaataaagc ccggcttgcc gacggcaacg atcaacagat
    27781 cggcattttc tacgtgatga cgcagatttt tagtgaagcg gtgagtcacg gtagtggtgc
    27841 aacctgctag cagcagttcc atgctcatcg ggcggccaac gatattcgat gcgccaatca
    27901 ccacggcgtt gaggccgaag gtatcaatgt tgtaacgctc aagcagcgtg acgatgccgc
    27961 gcggggtgca gggacgcaga cgcggcgcgc gctggcacag gcgaccgacg ttataaggat
    28021 ggaaaccgtc tacgtctttg tccgggtgaa tacgttccag tactttgacg ttatcaatac
    28081 ccgccggtaa cggcagttga accagaatgc catcaatggt gttgtcggca ttcagcgcat
    28141 cgataagctc cagcagctcc gcttcgctgg tggtttccgg gaggtcataa gagcgggaga
    28201 cgaacccgac ttcttcacaa gccttgcgtt tgcttgcgac ataaatttgc gatgcagggt
    28261 tactacccac cagcacaacg gccagtcctg gtgcccgcag tccggctgca atacgcgcct
    28321 gaactttttg agcaacttca gagcgcacct gctgcgcaat cgttttaccg tcaataatct
    28381 ttgctgccat cagagagagg attccatctg ttacgtaggt cgaaggggat gcgcctattt
    28441 tgtcagaagc ggggcgcgct gtcaggtttc gtttcagatt tatcacgtga agcgacctct
    28501 tgcgaaggtt aggcgcaccg tcgctgaggt cgctgagatt gaaagcttca tttttcgtcc
    28561 atgatggcgt tgtaaatctg gaactgactt atttccttgt ctaaggatta agataattta
    28621 agaaatacct gacaatataa aaagaatttt cagcctggta atttaccgct tcaggtctat
    28681 atttgtgttg aatatatttt gcgcggaagt attcatctaa cggggctctc tattttttag
    28741 aatagagtgc atattttcaa ttaagacatt cttagaggat aaaaaggaat ttactactat
    28801 cagtgtctta aataaagtaa tcggttatat acggatgtgg agtcgataaa tgagattgaa
    28861 ggaatatata tgaaattaag atttatttcg tctgcgctgg ctgccgcact attcgccgct
    28921 acgggtagtt atgctgccgt tgtagatggc ggtacaattc actttgaagg cgaactggtg
    28981 aatgctgtct gttcagtgaa tactgactcg gcagaccaag ttgtcacact cggtcaatat
    29041 cgtaccgata ttttcaatgc tgttggtaat acctctgcat taattccatt caccattcag
    29101 ttgaacgact gcgatcctgt tgttgccgct aatgctgccg ttgcattttc tggtcaggct
    29161 gatgcaatca atgataattt attggccatt gcatccagta ccaatacaac aacagcaacg
    29221 ggtgtcggta ttgaaatact tgataataca tccgcaattc tcaaacctga tgggaatagc
    29281 ttctcaacca accagaactt gatccccggg accaacgttc ttcatttttc tgcacgttat
    29341 aaaggcaccg gtacaagtgc atcagcaggg caagcaaatg ctgacgcgac ttttattatg
    29401 agatacgaat aatcaaaacc acgttgtttt gaattatata tcacgtctta taacaaagta
    29461 atgtaccggt tgtctgaagc ggtatggtgg caatgtaaat cgaaatcatg ttcactttgt
    29521 atcatgccgc tttattaaat gaaaagggaa tgatgtgttg taagaaacca aagcaatcat
    29581 ttctttatat ttcttatttt tgccgtcagg aatacacaag gcgtattaac tatgatgact
    29641 aaaataaagt tattgatgct cattatattt tatttaatca tttcggccag cgcccatgct
    29701 gccggaggga tcgcattagg tgccacgcgt attatttatc ccgctgatgc taaacagact
    29761 gcggtatgga ttagaaatag ccataccaat gagcgttttc tgatcaattc gtggattgaa
    29821 aacagcagcg gtgtaaaaga aaagtcattc atcattacac cgccactatt tgttagtgaa
    29881 cccaaaagcg aaaatacttt gcgtattatt tacaccggtc caccgctggc ggcagatcgt
    29941 gagtctctgt tctggatgaa tgttaagacg atcccttcgg tagataaaaa tgcattgaac
    30001 ggcaggaatg ttttgcaact ggcgatttta tcgcgcatga agttatttct ccgtccaatt
    30061 caattacaag aattacccgc agaagcgccg gacacactca agttttcgcg atccggtaac
    30121 tatatcaatg ttcataatcc atcacctttt tatgtcaccc tggttaactt acaagtgggc
    30181 agccaaaagt tggggaatgc tatggctgca cccagagtta attcacaaat tcccttaccc
    30241 tcaggagtgc agggaaagct gaaatttcag accgttaatg attatggttc agtaaccccg
    30301 gtcagagaag tgaacttaaa ctaaccgaat catctgacaa tatcagagct aattatgaaa
    30361 atacccacta ctacggatat tccgcagagg tatacctggt gtctggccgg aatttgttat
    30421 tcatctcttg ccattttacc ctccttttta agctatgcgg aaagttattt caacccggca
    30481 tttttattag agaatggcac atccgttgct gatttatcgc gctttgagag aggtaatcat
    30541 caacctgcgg gcgtgtatcg ggtggatctc tggcgtaatg atgagttcat tggttcacag
    30601 gatatcgtat ttgaatcgac aacagaaaat acaggtgata aatcaggtgg gttaatgccc
    30661 tgttttaacc aggtactcct tgaacgaatt ggccttaata gcagtgcatt tcccgagtta
    30721 gcccagcagc aaaataataa atgcatcaat ttactgaaag ctgtacctga tgccacaatt
    30781 aactttgatt ttgcagcgat gcgcctgaac atcactattc ctcagatagc gttgttgagt
    30841 agcgctcacg gttacattcc gcctgaagag tgggatgaag gtattcctgc tttactcctg
    30901 aattataatt tcactggtaa cagaggtaat ggtaacgata gctatttttt tagtgagctc
    30961 agcgggatta atattggccc gtggcgttta cgcaacaatg gttcctggaa ctattttcgc
    31021 ggaaatgggt atcattcaga acagtggaat aatattggca cctgggtaca gcgcgccatt
    31081 attccgctga aaagtgaact ggtaatggga gacggcaata caggaagtga tattttcgat
    31141 ggtgttggat ttcgtggtgt acggctttac tcttccgata atatgtatcc tgatagccag
    31201 caagggtttg ccccaacagt acgtgggatt gcccgtacgg cggcccagct aacgattcgg
    31261 caaaatggtt ttattatcta tcaaagctat gtttcccccg gcgcttttga aattacagat
    31321 ttgcacccga catcttcaaa tggcgatctg gacgtcacca tcgacgagcg cgatggcaat
    31381 cagcagaatt acactattcc gtattcaaca gtgccaattt tacaacgcga agggcgtttc
    31441 aaatttgacc tgacggcggg cgattttcgt agcggtaata gtcagcaatc atcgcctttc
    31501 ttttttcagg gcacggtact cggcggttta ccacaggaat ttactgccta cggcgggacg
    31561 caattatctg ccaattacac cgccttttta ttagggctgg ggcgcaacct cgggaactgg
    31621 ggcgcagtgt cgctggatgt gacccatgcg cgcagtcagt tagccgacga tagtcgtcat
    31681 gagggggatt ctattcgctt cctctatgcg aaatcgatga acaccttcgg caccaatttt
    31741 cagttaatgg gttaccgcta ttcgacacaa ggtttttata cccttgatga tgttgcgtat
    31801 cgtcgaatgg aggggtacga atatgattac gattacgacg gtgagcatcg cgatgaaccg
    31861 ataatcgtga attaccacaa tttacgcttt agccgtaaag accgtttgca gttaaatatt
    31921 tcacaatcac ttaatgactt tggctcgctt tatatctctg gtacccatca aaaatactgg
    31981 aatacatcgg attcagatac gtggtatcag gtggggtata ccagcagctg ggttggcatc
    32041 agttattcgc tctcattttc gtggaatgaa tctgtaggga tccccgataa cgaacgtatt
    32101 gtcggactta atgtttcagt gcctttcaat gttctgacca aacgtcgcta cacccgggaa
    32161 aatgcgctcg accgcgctta tgcctccttt aacgccaacc gtaacagcaa cgggcaaaat
    32221 agctggctgg caggtgtagg tgggacctta ctggaaggcc acaacctgag ttatcacgta
    32281 agccagggtg atacctcgaa taatgggtat acgggcagcg ccacggcaaa ctggcaggcc
    32341 gcttacggta cgctgggggt cgggtataac tacgaccgcg atcaacatga cgttaactgg
    32401 cagctgtctg gcggtgtggt cgggcatgaa aatggcataa cgctgagcca gcctttaggg
    32461 gataccaatg ttttgattaa agcgcctggc gcaggcggtg tacgcattga aaatcaaact
    32521 ggcattttaa ccgactggcg cggctatgcg gtgatgccgt atgccacggt ttatcggtat
    32581 aaccgtatcg cgcttgatac caatacgatg gggaattcca tcgatgttga aaaaaatatt
    32641 agcagcgttg tgccgacgca aggcgcgttg gttcgtgcca attttgatac ccgcataggc
    32701 gtgcgggcgc tcattaccgt tacccagggc ggaaaaccgg tgccgtttgg atcactggta
    32761 cgggaaaaca gtaccggaat aaccagtatg gtgggtgatg acgggcaagt ttatttaagt
    32821 ggtgcgccat tgtctggtga attactggtt cagtggggag acggcgcgaa ctcacgctgc
    32881 attgcgcact atgtattgcc gaagcaaagc ttacagcaag ccgtcactgt tatttcggca
    32941 gtttgcacac atcctggctc ataaaggaaa ttatcaatga agataatctg cagattatta
    33001 ttggcgatgg catgtttgtg tctggcaaac atatcctggg ctactgtttg tgcaaatagt
    33061 actggcgtag cagaagatga acactatgat ctctcaaata tctttaatag caccaataac
    33121 cagccagggc agattgttgt tttaccggaa aaatccggct gggtaggtgt ctcagcaatt
    33181 tgtccacccg gcacgctggt gaattataca taccgtagtt atgtcaccaa ctttattgtt
    33241 caggaaacta tcgataatta taaatatatg caattaaatg attatctatt aggtgcgatg
    33301 agtctggttg atagtgttat ggatattcag ttccccccgc aaaattatat tcggatggga
    33361 acagatccta acgtttcgca aaaccttcca ttcggggtga tggattctcg tttaatattt
    33421 cgtttaaagg ttattcgtcc ctttattaac atggtggaga tccccagaca ggtgatgttt
    33481 accgtgtatg tgacatcaac gccttacgat ccgttggtta cacctgttta taccattagt
    33541 tttggtggcc gggttgaagt accgcaaaac tgcgaattaa atgccgggca gattgttgaa
    33601 tttgattttg gtgatatcgg cgcatcgtta tttagtgcgg cagggctggg taatcgacct
    33661 gctggtgtca tgccgcaaac caagagcatt gcggtcaaat gtacgaatgt tgctgcgcag
    33721 gcttatttaa caatgcgtct ggaagccagt gccgtttctg gtcaggcgat ggtgtcggac
    33781 aatcaggatt taggttttat tgtcgccgat cagaacgata cgccgatcac gcctaacgat
    33841 ctcaatagcg ttattccttt ccgtctggat gcagctgcgg cagccaatgt cacacttcgc
    33901 gcctggccta tcagtattac cggtcaaaaa ccgaccgaag ggccgtttag cgcgctgggg
    33961 tatttacgcg tcgattatca atgaggtacg gagaatgaga agagtactct ttagctgttt
    34021 ctgcgggcta ctgtggagtt ccagtggatg ggcagttgac cctttaggaa cgattaatat
    34081 caatttgcac ggtaacgttg ttgatttctc ctgtaccgta aacacagcgg atattgataa
    34141 gacggtagat ttaggcagat ggcctacgac acaactactg aacgctggcg ataccacggc
    34201 actcgtccct tttagcctgc ggctggaggg atgtcctccg ggttcagttg cgattttatt
    34261 tacgggaacg ccggcatccg ataccaacct gctggctctg gatgatcccg caatggcaca
    34321 aaccgtcgcc atcgaattac gtaatagcga tcgctcccgg ctcgcactgg gggaggcgag
    34381 cccgactgag gaagtagatg caaatggcaa tgtcacacta aacttttttg ccaattatcg
    34441 agcgttagcc agcggtgttc ggcc
//
LOCUS       DBBGLQ010000046        34367 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.046, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000046 DBBGLQ010000000
VERSION     DBBGLQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.046"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(65..1006)
                     /gene="fabY"
                     /locus_tag="ACIYSB_002926"
                     /gene_synonym="yiiD"
     CDS             complement(65..1006)
                     /gene="fabY"
                     /locus_tag="ACIYSB_002926"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGC5429936.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1051..1488)
                     /gene="dtd"
                     /locus_tag="ACIYSB_002927"
     CDS             complement(1051..1488)
                     /gene="dtd"
                     /locus_tag="ACIYSB_002927"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGC5429937.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1485..2357)
                     /gene="yihY"
                     /locus_tag="ACIYSB_002928"
     CDS             complement(1485..2357)
                     /gene="yihY"
                     /locus_tag="ACIYSB_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGC5429938.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2351..2950)
                     /gene="yihX"
                     /locus_tag="ACIYSB_002929"
     CDS             complement(2351..2950)
                     /gene="yihX"
                     /locus_tag="ACIYSB_002929"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGC5429939.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3049..3834)
                     /gene="csqR"
                     /locus_tag="ACIYSB_002930"
     CDS             complement(3049..3834)
                     /gene="csqR"
                     /locus_tag="ACIYSB_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGC5429940.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3868..4764)
                     /gene="yihV"
                     /locus_tag="ACIYSB_002931"
     CDS             complement(3868..4764)
                     /gene="yihV"
                     /locus_tag="ACIYSB_002931"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGC5429941.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4932..5828
                     /gene="yihU"
                     /locus_tag="ACIYSB_002932"
     CDS             4932..5828
                     /gene="yihU"
                     /locus_tag="ACIYSB_002932"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGC5429942.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5852..6730
                     /gene="yihT"
                     /locus_tag="ACIYSB_002933"
     CDS             5852..6730
                     /gene="yihT"
                     /locus_tag="ACIYSB_002933"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGC5429943.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6747..7988
                     /gene="yihS"
                     /locus_tag="ACIYSB_002934"
     CDS             6747..7988
                     /gene="yihS"
                     /locus_tag="ACIYSB_002934"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HGC5429944.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8126..9028
                     /gene="yihR"
                     /locus_tag="ACIYSB_002935"
     CDS             8126..9028
                     /gene="yihR"
                     /locus_tag="ACIYSB_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGC5429945.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQLWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLESGKPHRLFFNIYGQRK"
     gene            9227..11263
                     /gene="yihQ"
                     /locus_tag="ACIYSB_002936"
     CDS             9227..11263
                     /gene="yihQ"
                     /locus_tag="ACIYSB_002936"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGC5429946.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            11309..12694
                     /gene="yihP"
                     /locus_tag="ACIYSB_002937"
     CDS             11309..12694
                     /gene="yihP"
                     /locus_tag="ACIYSB_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5429947.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPASVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            12737..14140
                     /gene="yihO"
                     /locus_tag="ACIYSB_002938"
     CDS             12737..14140
                     /gene="yihO"
                     /locus_tag="ACIYSB_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5429948.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILIGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            14208..14900
                     /gene="ompL"
                     /locus_tag="ACIYSB_002939"
     CDS             14208..14900
                     /gene="ompL"
                     /locus_tag="ACIYSB_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HGC5429949.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYNFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(14991..16256)
                     /gene="yihN"
                     /locus_tag="ACIYSB_002940"
     CDS             complement(14991..16256)
                     /gene="yihN"
                     /locus_tag="ACIYSB_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5429950.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFSGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(16358..17338)
                     /gene="yihM"
                     /locus_tag="ACIYSB_002941"
     CDS             complement(16358..17338)
                     /gene="yihM"
                     /locus_tag="ACIYSB_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGC5429951.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(17346..18056)
                     /gene="yihL"
                     /locus_tag="ACIYSB_002942"
     CDS             complement(17346..18056)
                     /gene="yihL"
                     /locus_tag="ACIYSB_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGC5429952.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(18273..20096)
                     /gene="typA"
                     /locus_tag="ACIYSB_002943"
     CDS             complement(18273..20096)
                     /gene="typA"
                     /locus_tag="ACIYSB_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGC5429953.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20469..21878
                     /gene="glnA"
                     /locus_tag="ACIYSB_002944"
     CDS             20469..21878
                     /gene="glnA"
                     /locus_tag="ACIYSB_002944"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGC5429954.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22164..23213
                     /gene="glnL"
                     /locus_tag="ACIYSB_002945"
     CDS             22164..23213
                     /gene="glnL"
                     /locus_tag="ACIYSB_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGC5429955.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23225..24634
                     /gene="glnG"
                     /locus_tag="ACIYSB_002946"
     CDS             23225..24634
                     /gene="glnG"
                     /locus_tag="ACIYSB_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGC5429956.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24746..24856
                     /gene="yshB"
                     /locus_tag="ACIYSB_002947"
     CDS             24746..24856
                     /gene="yshB"
                     /locus_tag="ACIYSB_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGC5429957.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25085..26458)
                     /gene="hemN"
                     /locus_tag="ACIYSB_002948"
     CDS             complement(25085..26458)
                     /gene="hemN"
                     /locus_tag="ACIYSB_002948"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGC5429958.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26647..27156)
                     /gene="yihI"
                     /locus_tag="ACIYSB_002949"
     CDS             complement(26647..27156)
                     /gene="yihI"
                     /locus_tag="ACIYSB_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGC5429959.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(27275..27441)
                     /locus_tag="ACIYSB_002950"
                     /pseudo
     CDS             complement(27275..27441)
                     /locus_tag="ACIYSB_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27738..28370
                     /gene="yihA"
                     /locus_tag="ACIYSB_002951"
     CDS             27738..28370
                     /gene="yihA"
                     /locus_tag="ACIYSB_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGC5429960.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28751..31537)
                     /gene="polA"
                     /locus_tag="ACIYSB_002952"
     CDS             complement(28751..31537)
                     /gene="polA"
                     /locus_tag="ACIYSB_002952"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGC5429961.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            31901..32833
                     /gene="yihG"
                     /locus_tag="ACIYSB_002953"
     CDS             31901..32833
                     /gene="yihG"
                     /locus_tag="ACIYSB_002953"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGC5429962.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(32874..34304)
                     /gene="yihF"
                     /locus_tag="ACIYSB_002954"
     CDS             complement(32874..34304)
                     /gene="yihF"
                     /locus_tag="ACIYSB_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGC5429963.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8358 a         8800 c         8430 g         8779 t
ORIGIN      
        1 gaaatagcca aggactaata taaatgacct gaatgagaga ggcagcaccg cccctctcca
       61 tcagctactc ttcttcgttc ccgccctctt catacgggcc aaatggcttc gcgggcagaa
      121 cgatatacgt gccttcaaac actgcacccg gcgtctcgtc gccaaagatt tcgacctgca
      181 tctgcacccg tgcttttcgt ccgcgcgcca gacggtcgag atcgccgctt aaggcaccga
      241 ggtcggctac cgcatgaggt ttaccgctaa tcggcttgct gtagcggata tgcgcatccg
      301 caagaataat cgttccgccg aggtggcgtt cacgcagcat cagccagata agtccccaac
      361 cggtgagcgt cgccagtgag aataaactcc cggcaaacag cgtatggtgc ggattctgat
      421 tgccggtttc tggcatggta gtgataaatt tttgcccggt atattgctga atgcgcacgc
      481 ccattttttc actaagcggg atatgttcgt accacgcctg ttgcagctgc gcgcaccagt
      541 cgccgcgatg cagaatgtca tccagagtgg cgacgggctt aatcatcaaa aaatggcgaa
      601 tcggcgtggt ggttggcgtg gtgatttctc cctgattaac aaaccccagc ttggcgaaaa
      661 actccaccgc gtcttcacgg gcgctacagg tcacgcgctt aacgccttcc tgacgcgcca
      721 ccgactccag ggtcatcgcc atcagcgtgc ctaacccttt gtcctgcacg tcgggatgaa
      781 cggccataaa gcgaatggac gcttcattgt cggcattaat atacagtcgg cctaccgcca
      841 ccagattacc ctgctcgtcg acgaccatct ggtgatgcgc catcgcatcc cacgcgtcgc
      901 gttccgaacc ttttggttga tgcaggggct tacgcaacat ttcccagcga aactgatagt
      961 aacgctctaa ttcttcttct gtttgtggaa cccgaaggtg atacatagcg atactctctc
     1021 ttgtttcccg tgacaaccct ggaagctggc tcatacctgc aaccagaatg tcacggggcc
     1081 atcattgacc agcgatacct gcatatccgc agcgaagcgt cctgtttgcg tgttcatctc
     1141 ttgctgacgg cagcgttcga cgaaatagtc atataacgcc tctgcgcgat ccggtgatgc
     1201 acctttggag aaacttgggc gcatcccccg ttcggtatct gcggcgaggg taaactggga
     1261 aaccaccagc acactgccgc ccgcctgttg cacgttgaga ttcatcttgc cttcggcatc
     1321 gctaaagatg cggtagccga gcacacgctc gcacagacgg tttgctttct gttcgtcgtc
     1381 atccttttcg acacccaata acaccaaaag tcccgcgcca atttcgcccg tcacttctcc
     1441 ctccacggtg acgctggcac gggttacgcg ttgaattaat gcaatcatgg ttcgtcgtct
     1501 tcttcttgtt cagctgcttg ttttagtttg cggtattccc cgagagtgac agtaatttcc
     1561 gcgccaagca agacgataca ccatgtccag tagacccaaa caaagagaat ggggatcacc
     1621 gccagcacac cataaatgag ctgatatgac gggaacatgg tgatataaag cgcgaaacct
     1681 ttctttcctg cttcgaacag gagtgcggcg acaaacgcgc cgacaatcgc gtcgcggtta
     1741 ggtacgcgga tggtaggaac aatgctgtac agcaaccaga aggagatcca cgacaacagc
     1801 aacggaaaaa tacgcagcac gttgtcgatg acagtattga gatcgctcgc ccagcgcagg
     1861 gagagcaaat aggaactgat cgccagactg gcccctgcca gcagcggccc cagcgttaaa
     1921 atcatccagt acacggcgaa cgagtaaatt tttggtcgcg ctcgtttact gcgccagatg
     1981 gtattcaacg cgctatcgat ggagtacatc aacaataacg cggtgacgat cagcccgcac
     2041 gccccaacgg cggtcatctt gttggaattg gcaacaaatt gttcgatata ccgctgaata
     2101 acatcgccag tagcaggcag aaagttggca aaaataaagt gacgtaactg aatgctgacg
     2161 tcggaaaaca tgggaaaagc ggcgaaaagc gcaaaaacaa cggcaaccag cggcactaat
     2221 gagagcaacg acacataggc aaggttacct gccagggttg tcatgttgtc ctcatcaatg
     2281 cgttgccaga gtagttttag ccaggcccat agtggacggg tacggtgcct ggctttgtcc
     2341 tgaatggttt ttagcataac accttcgcga aatagtccgg gatggtggtt ttatctttca
     2401 ccagaatact ggtaatgccc agctgattgg ctccttctat attatcggcg ttatcgtcga
     2461 aaaagaccgt atcgctgggt gaaaaacctt ccgcctgcaa aacatgctgg taaattcgtg
     2521 cttcaggttt gcgcatcccc agatcttgcg acagatagat atggtcagca gcatcacgaa
     2581 tttccgggta ttcttccggc cagaaggtgg tatgcaggcg gttggtattg gaaagcacca
     2641 ccacgcgatg cccctgctca cgcagtttat gcatgatggc gatcacttcc gggcgcagcg
     2701 caacaaacac cgcctgccag ccgtgagaga actgctcgta gcttagcggt agagccatct
     2761 catgacacag cgcctctgcg aacgcttcgt cgctaatttc cccacgctca tgctgatgaa
     2821 acgcctcccc catatgaaaa ctcttcttaa gcgatgccag cggaatacgc gttaaatcgc
     2881 tccaggctcc cagcacacgg ttaaagtcga tatcgacaat cacattacct aaatcaaaga
     2941 tatagagcat ttttgcctcc tttcgcgcca caaaaaatta actgtagcgg gaaaggatgg
     3001 gtttggctat gtggcaaggt atgaagccag gttaacggtc acaaaaatct acgcgtcttc
     3061 ctggggaaga atgatttcaa tttggctgtc ttgtaagcgt gattttagcg cagcgggcgg
     3121 cagctcgtcg gtaaagagcg cagtgacctg tgccacgtta ccaatttcaa ccgccgccga
     3181 agcatgatac ttagtgtgat cggcgaccag cagaatattt ctcgcgtgcg ccatcatcgt
     3241 tttcaccacg ttagcttcgt ttacatcaaa ctccatcaac gcgccatcgc tctcaatcgc
     3301 cccaacgctt gttaccagat aatcagcacg aaaatcagcc acaaaggacg ccgctgaagg
     3361 gccaatgatc ccgctattat gagagcgcaa cgtaccgccg ggcaccatca cttcaaagcg
     3421 cgggttgtgg taaagaatat gcgccacacg caggctgttg gtgattatcc gcaaatgatt
     3481 atggttaagt aacgcccggg caacatgctc aacagtcgta ccaatggtga taaatattgt
     3541 tgaaccatca ggaatatagt ctgccaccgc ttcggcaatc gctttttttt cctcggtttg
     3601 cgaaacctca cgctgctcga acgccgtatt aacgacgctg gaagcccgac ccgcgccacc
     3661 gtgatggcgc gtaatcaggc cttgctcgct taatttacga atatcccggc ggaccgtctg
     3721 cgtggagaca tccagcagat ttgccagctc atcaatattc atatacccac gctcggcgat
     3781 cagcatgagg agttggtcgt gccgcgggtt accggttagt tcggtaaggc tcatgaaaat
     3841 tcctcgaaaa accatcaccc tggcatttta tacaaaaagt gacaaaaaag atcgggtttg
     3901 atcacagtca gggatcccgg ctcgtccacc gggacgtgtg cattttaacg ccgctacacc
     3961 gctggcgaag cggactgact ccgctaaatc cccacttgtt gccagcgcca ccgccaaagc
     4021 gccgtgaaaa acatcacccg cacctgtggt atctaccaca tcaactttga aggccggttg
     4081 atgctgacgc ccaccatttt ccagccagtc gcagcctgcg ctaccctggg tcacatagac
     4141 atgaccattt gtgagcgttt gtgcctgttt tagcgcactg gccatctctt tcacgcccgt
     4201 taagcgcgcc agacccggtt ctgaaaaggc cgcgtgatcg cttaatgcca ccagctcact
     4261 gatatcctgc ggcgtaatgt ccccgtccag aacggtcatc acacccgcct gacgggccag
     4321 ggtgaaggct tttttagcgc cgtcgtgcca gcgtacatct gccagcacaa catcccactg
     4381 agagaaatca atttcctcca accactctgc gtcaggcagc agatccgggc tggggtagtt
     4441 aatgattatc cgctcgcctt tggtatccac catgatggcg gattgcgaag atttcgcctg
     4501 gttataccgt ttggtgtaac gggtgttaac gccccaggat tccagctctg ccagcaggct
     4561 gttgcccgtg tcgtcatcac ctacgcgacc aataaaatcg acctgcgccc ccagccttgc
     4621 cgccgcaacc gctgccgtcg ccgctggccc gccaccaact tccgtataat ttctcgccac
     4681 gtatttaccg ctctccgtcg gtaacccttc cacgtaatag atgcgatcca tcacggttat
     4741 acctacacaa gcaacacgaa tcatggtcat tccttaaaca ttttgggatg cgttcatttt
     4801 aatttcacca attgtttaaa aagtgacggc tgtcaatttt ttgacgataa attacaaata
     4861 cgatcaaaaa cagacaagca aagcatgtca taaatgacaa aaagtgacat cactgtattc
     4921 aggagagggt tatggcagca atcgcgttta tcggtttagg acaaatgggt tcgccaatgg
     4981 cgagcaattt attgcagcaa gggcatcaac ttcgcgtctt tgatgtgaac gccgaagctg
     5041 tgcggcatct ggtagacaaa ggtgcgactc ccgccgccaa cccggcgcag gccgctaaag
     5101 atgccgaatt tatcattacc atgttgccga atggcgatct ggtgcgcaac gtgttgttcg
     5161 gtgaaaacgg cgtttgcgaa ggcttatcta ccgatgcgct ggtcattgat atgtccacca
     5221 tccatccgct gcaaaccgat aaattgattg ccgatatgca agccaaaggc ttcagcatga
     5281 tggatgttcc ggtaggccgt acttctgcaa atgccattac cggtactctg ttactgctgg
     5341 ctggcggcac cgctgaacaa gttgaacgtg ccacaccgat cctgatggcg atgggcagtg
     5401 agttgatcaa cgctggcggt ccgggcatgg ggatccgcgt taagctcatc aacaactaca
     5461 tgagcatcgc gctcaatgcg ctttcggcag aagccgccgt tttgtgcgaa gccctgaatc
     5521 ttcccttcga tgttgccgtc aaagtgatga gcggtaccgc cgccggtaaa ggtcacttca
     5581 ccacttcctg gccaaacaaa gtcctcagcg gagatctttc tcccgccttc atgatcgatc
     5641 ttgcccataa ggatcttggc atcgcccttg atgtcgccaa ccagctgcat gtgccaatgc
     5701 cgctgggggc cgcctcacgg gaggtttata gccaggcgcg cgcagcgggt cgcggtcgcc
     5761 aggactggtc cgccattctg gaacaggtcc gtgtcagtgc cgggatgact gccaaagtaa
     5821 aaatgtaacg actggataaa ggaataaatg aatgaataag tacaccatca acgacattac
     5881 gcgcgcatcg ggcggttttg ccatgctggc ggtcgatcag cgcgaagcca tgcgcatgat
     5941 gtttgccgcg gctggggcac ccgctccggt agccgatagc gttttaactg atttcaaagt
     6001 taacgctgca aaggccctct cgccttatgc ctcggcgatt ctggtagatc aacaattctg
     6061 ctaccgccag gtggttgagc aaaacgcgat tgccaaaagt tgcgccatga ttgtcgccgc
     6121 cgatgagttc attcctggca acggtattcc ggtcgatagc gtggttattg accgcaaaat
     6181 caatccgcta cagatcaaac aagacggtgg caaagcctta aaactgctgg tgctgtggcg
     6241 tagtgatgaa gatgcgcagc aacgtctgga tatggtgaaa gagttcaacg aactgtgcca
     6301 ctcacacggt ctggtaagca tcattgagcc agtcgtccgc ccaccgcgtc gtggcgataa
     6361 attcgatcgc gaacaagcga tcatcgatgc cgccaaagag ctgggcgaca gtggcgctga
     6421 cctctacaaa gttgaaatgc cgctttatgg caaaggtccg caacaagagc ttctctgtgc
     6481 ttcacaacgt ctgaatgacc atatcaatat gccatgggtg atcctctctt ccggtgtcga
     6541 cgaaaaactg ttcccgcgtg ccgtacgtgt ggcaatgacg gcgggcgcat cgggattcct
     6601 ggcaggccgt gcagtctggg catcggtcgt cggtttacca gacaacgagc tgatgctgcg
     6661 tgacgtttgc gcaccgaaat tacaacaact tggcgatatc gtcgacgaaa tgatggctaa
     6721 acgccgctaa gatgaggata aaaggaatga aatggtttaa caccctaagc cacaaccgtt
     6781 ggctggaaca ggaaaccgac cgcatctttg attttggtaa aaattccgta gtgccgactg
     6841 gttttggctg gttaggcaat aaagggcaaa tcaaagaaga gatgggcacc catctgtgga
     6901 tcaccgctcg tatgttgcac gtttattccg ttgctgcggc gatgggtcga cctggcgctt
     6961 actcgttggt tgatcacggt atcaaagcca tgaacggcgc actgcgtgat aaaaaatatg
     7021 gcggctggta tgcctgcgtg aatgacgagg gcgtggtgga tgcctccaaa cagggctatc
     7081 aacacttctt tgctctgctg ggtgccgcca gcgccgtcac aacgggtcac ccggaagcgc
     7141 gcaagctgct cgattacacc attgaaatta tcgagaaata tttctggagc gaagaagagc
     7201 agatgtgcct ggaatcctgg gacgaagcct tcagcaaaac cgaagagtac cgcggcggca
     7261 atgccaatat gcacgcggtg gaagctttct tgattgttta tgacgtcact cacgacaaaa
     7321 aatggctgga tcgcgcgatt cgcgtggctt ccgtgattat ccacgacgtc gccagaaata
     7381 atcattatcg cgttaacgaa cacttcgata cccagtggaa tccgctgccg gattacaaca
     7441 aagataaccc ggcgcatcgc ttccgcgcgt tcggaggtac accaggccac tggatcgaat
     7501 ggggccgttt aatgctgcac atccatgctg ccctggaagc ccgttgcgaa caaccaccag
     7561 catggctgct agaagatgcc aaaggtctgt ttaacgccac cgtgcgcgat gcctgggcac
     7621 ccgatggcgc ggacgggatt gtttataccg ttgactggga aggaaaaccg gtggtccgcg
     7681 aacgtgtacg ttggcctatc gtcgaagcaa tgggtacggc ctacgcgctc tacaccgtca
     7741 ctggcgatcg ccagtatgaa acctggtatc aaacatggtg ggagtactgc attaagtacc
     7801 tgatggacta tgaaaatggt tcctggtggc aggagctgga tgcggacaat aaggtcacca
     7861 ccaaagtctg ggacggcaaa caggatattt atcacctgct gcattgcctg gtgatcccgc
     7921 gtatcccgtt agcgcccggc atggctccag cggttgcagc gggtctgctg gatattaatg
     7981 cgaaataact gatctgaaaa tagggtgcgg gttttcccct ctccctaacc ctctccccaa
     8041 aggggcgagg ggaccgtccg gtgcagtttt tgggtttgac atcagcctca cgggaggctg
     8101 tatgtcgtta attaaggtgc cttgtatgca aatcaccaat atgcattgca gtggacagac
     8161 cgtttcactt gccgcggggg attatcacgc gaccattgtg accgtcggtg ctggtctggc
     8221 tgaattaacc tttcagggtt gtcatttagt catcccgcac aaacccgaag agatgccgct
     8281 ggcgcatctg ggtaaagtgc ttattccgtg gccgaaccgg atcgccaacg gctgttaccg
     8341 ctatcagggg caggaatatc agttacccat taatgaacat agttcaaaag ccgctattca
     8401 cgggctgctt gcctggcgag actggcaaat tagtgaatta accgccacca gcgttaccct
     8461 gacagctttt ctgccgccca gttacggcta tccgtttatg ctggcttcac aagttgttta
     8521 ttcgctcaac gcacacacgg gtctttccgt tgagatcgca agccagaata tcggcactgt
     8581 cgcagcgcct tatggcgtgg gtattcatcc ttatttaacc tgcaacttaa catctgttga
     8641 tgaatatctg ttccagcttc cggcaaacca ggtttatgcc gtcgatgagc acgcgaatcc
     8701 gacaacgctt caccacgttg atgagctgga tttaaatttc acgcaggcga aaaaaatagc
     8761 cgcgacaaaa atcgaccaca cctttaaaac cgctaatgat ttgtgggaaa tgaccattac
     8821 ccatccacag caggcgcttt cggtaagcct ttgttccgat caactctggg tgcaggttta
     8881 tagcggtgaa aaattacagc gtcagggact ggcagttgaa cctatgagtt gcccgcctaa
     8941 cgcctttaat tcaggtatcg acttattatt actggaatca ggaaaaccgc accgcctgtt
     9001 ttttaatatt tacggacaga gaaaataact tgtattgatt agcagtcaaa atcattgaag
     9061 aacatttcac tcgctaaagg caacattatg tatcttcaat ttattaagtt aaaaacagct
     9121 cagcttctgt aaagttttta tatatcagtt ttatgagaat cattttacat aagcctgtaa
     9181 taacaggaag gcaacaatct cgttctcaac tctatggaga ttagttatgg atacgccacg
     9241 tccacagtta ttagattttc aatttcatca gaataacgac agttttaccc tacattttca
     9301 acaacgtctt attttaaccc atagcaaaga taatccttgt ttatggattg gctcaggtat
     9361 agcggatatc gatatgttcc gcggtaattt cagcattaaa gataaactac aggagaaaat
     9421 tgcgcttacc gacgccatcg tcagccagtc accggatggt tggttaattc atttcagccg
     9481 tggttctgac attagcgcca cgctgaatat ctctgccgac gatcaggggc gtttattgct
     9541 ggaactacaa aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg ccgctcaacc
     9601 agaggaccat atctacggct gcggcgaaca gttttcctac ttcgatctgc gtggcaaacc
     9661 gttcccgcta tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg
     9721 gcaggccgac tgcaaagaaa atgcgggcgg cgactattac tggactttct tcccacagcc
     9781 tacgtttgtc agcacgcaga agtattactg ccatgttgat aacagttgct atatgaactt
     9841 cgactttagt gccccggaat accatgaact ggcgctgtgg gaagacaaag caacgctgcg
     9901 ttttgaatgt gctgacacat acatttccct gctggaaaaa ttaaccgccc tgctgggacg
     9961 ccagccagaa ctgcccgact ggatttatga cggagtaacg ctcggcattc agggcgggac
    10021 ggaagtgtgc cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg tcaacggcat
    10081 ctgggcgcag gactggtccg gtattcgtat gacctctttt ggcaaacgcg tgatgtggaa
    10141 ctggaagtgg aacagcgaaa actacccgca actggattca cgcattaagc agtggaatca
    10201 ggagggcgtg cagttcctgg cctatatcaa cccgtatgtt gccagcgata aagatctctg
    10261 cgaagaagcg gcacaacacg gctatctggc aaaagatgcc tctggcggtg actatctggt
    10321 ggagtttggc gagttttacg gcggcgttgt cgatctcact aatccagaag cctacgcctg
    10381 gttcaaggaa gtgatcaaaa agaacatgat tgaactcggc tgcggcggct ggatggctga
    10441 cttcggcgag tatctgccca ccgacacgta cctgcataac ggcgtcagtg ccgaaattat
    10501 gcataacgcc tggcctgcgc tgtgggcgaa gtgtaactac gaagcccttg aagaaacggg
    10561 caagctcggc gagatccttt tctttatgcg cgccggttct accggtagcc agaaatactc
    10621 caccatgatg tgggcgggcg accagaacgt cgactggagt ctcgacgatg gcctggcgtc
    10681 ggttgtcccg gcggcgctgt cgctggcaat gaccggacat ggcctgcacc acagcgacat
    10741 tggcggttac accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg
    10801 cgatttcagc gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc ctggcgacaa
    10861 ctggcagttt gacggcgacg cagaaaccat cgcccatttc gcccgtatga ccaccgtctt
    10921 caccaccctg aaaccttacc tgaaagaggc cgtcgcgctg aatgcgaagt ccggcctgcc
    10981 ggttatgcgc ccgctgttcc tgcattacga agacgatgcg cacacttaca ccctgaaata
    11041 tcagtacctg ttaggtcgcg acattctggt cgctccggtg catgaagaag gccgtagcga
    11101 ctggacgctc tatctgccgg aggataactg ggtccacgcc tggacgggtg aagcgttccg
    11161 gggcggggaa gttaccgtta atgcgcccat cggcaagccg ccggtctttt atcgcgccga
    11221 tagcgaatgg gcggcactgt tcgcgtcgtt aaaaagcatc taatcacatc cgcccgtggg
    11281 gaaactcatg ggcaaaggga gaacatcgat gagtcacatc acaacggaag atccagcaac
    11341 tttacgcctg ccctttaaag agaaactctc ttacggtatt ggcgacctgg cctctaacat
    11401 cctgctggat atcggtacgc tttatctttt gaagttttat accgacgttc tggggctgcc
    11461 aggcacctat ggcggcatta tctttttgat ttcaaaattc tttactgcgt ttaccgatat
    11521 gggtaccggc attatgttgg attcccgacg caagatcggt ccaaaaggta agttccgtcc
    11581 ttttattctg tatgcgtcat tcccggtcac cttactggcg atcgccaact ttgtcggcac
    11641 accgtttgat gtcaccggta aaacggtgat ggccactatt ctgtttatgc tttacggact
    11701 gtttttcagc atgatgaact gctcctacgg cgcaatggtt cccgctatca ccaaaaaccc
    11761 caacgaacgc gcctcactgg cggcatggcg tcagggcggc gctacgctgg gcctgctgct
    11821 gtgcacggtg ggattcgtgc cagttatgaa tcttatcgaa ggtaatcagc aacttggcta
    11881 tatcttcgcc gccacgctgt tttcactgtt tggcctgctg tttatgtgga tctgctactc
    11941 gggcgtgaaa gagcgttatg tcgaaaccca gcctgctaat ccggcgcaaa agccgggcct
    12001 gctgcaatct ttccgcgcaa ttgcgggtaa ccgcccgctg ttcattctgt gcattgccaa
    12061 cctctgcact ttaggggcgt ttaacgtcaa gctcgccatc caggtctatt acacccagta
    12121 cgtgcttaac gatcccatcc tgttgtcgta tatgggattt ttcagcatgg gctgtatttt
    12181 catcggcgta ttcctgatgc ctgcctcagt cagacgtttt ggcaagaaga aagtttatat
    12241 cggcggcctg ctgatttggg tgctgggcga tctgctcaac tatttcttcg gcggcggttc
    12301 ggtcagcttc gtggcgttct cctgcctggc gttctttggc tcagcgtttg ttaacagcct
    12361 gaactgggcg ctggtttccg acaccgtcga gtacggcgag tggcgcaccg gcgtgcgttc
    12421 ggaaggaacg gtctacaccg gttttacctt ctttcgcaaa gtgtctcagg cgctggctgg
    12481 tttcttcccc ggctggatgc tgacgcaaat tggctatgtg ccgaacgtcg cacaggctga
    12541 ccacaccatt gaagggttac gccagttgat cttcatctac ccaagcgcac tggcggtagt
    12601 caccattgtg gcgatgggtt gcttctacag cctgaacgag aagatgtatg tccgcattgt
    12661 ggaagagata gaagcccgta aacgcacggc gtaataataa taaacgacgc cctgcggggc
    12721 gttataagga gtgattatgt ctgaccataa tccactgaca ttaaaactga atctgcggga
    12781 aaaaatcgcc tatggtatgg gcgacgtcgg ttcgaattta atgctctgca tcggtactct
    12841 gtatctcctc aaattttata ccgatgaatt agggatgcct gcttactatg gcgggattat
    12901 ctttctggtg gcgaagtttt ttaccgcgtt taccgatatg ctcaccggat ttttactcga
    12961 ctcgcggaaa aatattgggc ctaaaggcaa atttcgtccc tttattttat atgcagcggt
    13021 tccggcggcg ttaattgcaa cgctccagtt tatcgccacc accttttgtt tgccggttaa
    13081 aacgacgatt gccaccgcgc tgtttatgat gtttggactt tcatacagtc tgatgaactg
    13141 ctcgtatggt gcgatgatcc cggcaattac caaaaacccg aatgagcgcg cgcagctcgc
    13201 ggcttaccgc cagggtggtg caaccatagg gctattgatt tgtaccgtgg cgtttattcc
    13261 gttgcagtcg cttttttctg actcaaccgt cggttatgcc tgtgcggcac ttatgttctc
    13321 cattggcggc tttattttta tgatgctgtg ctacagaggc gtcaaagagc attatgtgga
    13381 cacaacgcca accggacata aagccagtat tctcaaatca ttttgcgcga tatttcggaa
    13441 tccgccattg ctggttttat gcattgctaa cttgtgtacc ctggcggcat ttaatatcaa
    13501 actggcgatt caggtctatt acacccagta tgtgctgaat gatattaatt tattgtcgtg
    13561 gatgggattc ttcagtatgg gatgcatcct catcggcgta ttactggtgc cattaactgt
    13621 aaaatgtttt ggtaaaaaac aggtttatct ggctggcatg gtgctgtggg cggtgggtga
    13681 tatactgaat tatttctggg gaagtaactc tttcactttc gtcatgttct cttgtgtcgc
    13741 cttttttggc acggcctttg taaacagcct gaactgggca ctggtaccag ataccgtaga
    13801 ttacggtgaa tggaaaaccg gtattcgtgc cgaaggttct gtttataccg gttatacctt
    13861 ctttcgtaaa atttctgccg cacttgctgg cttcttgcca ggcattatgc tgacgcaaat
    13921 tggttatgtt cccaacatcg cgcaaagcga tgcgacatta cagggtttgc gtcagctcat
    13981 ttttatctgg ccttgtgcac tggcaattat tgctgcattg acgatgggat tcttttacac
    14041 actcaatgaa aaacggtttg cattgattat tgaggaaatc aaccaacgca aaaataaaga
    14101 gatggcgaca gaagaaaaaa cggcttccgt aacgttataa atgtcaatag ccgctatttc
    14161 catcctggtg ggtggcggcc tccctacgtt taaaaaatgg acttattatg aaaaagatta
    14221 atgcaataat tttattatct tctctgacct ctgcctcggt atttgctggc gcatacgtcg
    14281 aaaacaggga ggcatataat cttgcctccg atcagggcga ggttatgcta cgtgtgggtt
    14341 ataacttcga tatgggcgcg ggtattatgt taaccaatac ctacaacttt cagcgagaag
    14401 atgaactaaa acatggatat aacgaaattg aaggctggta tccgttattt aaaccaaccg
    14461 ataaattaac catccagccc ggtggcttaa ttaatgataa gagtatcggt tcgggtggtg
    14521 cagtgtatct ggacgtcaac tataaatttg taccatggtt taatctgaca gtacgaaatc
    14581 gctataacca taataactat agttcaacag atttgagcgg ggaactggat aataatgaca
    14641 cctatgaaat tggcacctac tggaatttta aaatcaccga taaattttcc tatacatttg
    14701 agccacatta cttcatgcga gtgaatgact ttaatagtag caacgggaaa gatcatcatt
    14761 gggaaatcac taacaccttc cgttaccgta ttaatgaaca ctggctccct tatttcgaat
    14821 tgcgctggtt agaccgcaac gtcgaaccgt accaccgcga gcaaaaccag atccgtatcg
    14881 ggacgaagta tttcttctga tgtgaaactg ccggatgcgg cgtaaacgcc ttatccggcc
    14941 tacggttgat acggttttcg ttggccggat taagcacatc cggccattaa ttaagcttca
    15001 gccgccagca tctctttctt acgttggcta atacgcttaa ccagcattac ggaaaccacc
    15061 gcaccactga atgcgaagca ggccataatg ccaaacacga ttttgtagcc aataatcccc
    15121 ggatttaaat ccagaatgta gccatacaga ctgaagcaga acatcgccgg agcgtaacca
    15181 ataaagctac ccaacgccat cgccgcacct gttttatttt cagcaatttt tgcttcgccg
    15241 ataggtgcaa aaaatacggc tcgctgtgta aagactatcg cgccaaagcc cagcgtacat
    15301 gccatcccta aatagaccgg catactttcg tgcggcagca taatcaacag tacgagcgca
    15361 gcggtactaa tgataaaggt gtagcataga tatttacttg gcgatttcag gatcttatct
    15421 gaaatcatgc caccaatcgg tccaccaatc attttcagac agtattggtt aatgatgccg
    15481 taagccccca ccagcgcaac gggcaataga tagatgtttt tcaggaatgg aatgaagaat
    15541 gtcaggccac agtaaaccgc gtaaacgaag aagacgttaa aagcgataag ccagatagtt
    15601 ttgtctttca gcaccgaggt catactggtg tttttcgatg ctccgtcttc ttttttcacc
    15661 tcaacggacg gtgcctcttc tttgtcattc aggacaaaga aaataataat gcctacggca
    15721 atcacaataa gggaatagaa ccagatgcct gctttaaaac ctaataagcc actgccaaac
    15781 caggtaaata ctgccaacgc agaaaatgcc accacggtat cgacaatgcc acgccctgtt
    15841 tcgaagaagc caaacaaccg accttgttgt tcgctattgc ccaatcgact taccgacttg
    15901 agcaagaccg gccagttcat catgtcgcaa gtaacgccaa atagcgccca gacaaagagg
    15961 atgccccaat accccggcat tgtcgtcaaa taaacaccca gtaatcctgt cgcaatgagt
    16021 gagaatgaca tggtgtatct gcgcggtagt ttatcggcaa aataaataga cagaaaaaag
    16081 cccactgtgg tgacaaatga gtttaccgac atagcattac caatttgacc attggtcaaa
    16141 tggaaatatt cctgcatcgg gatataaaac gcatctttca gcgacggtaa tttataaatt
    16201 gtaccgccac acagtgttaa tagactaaat aacgcccatt tctttttcgt gagcataaca
    16261 tcccctaagc gatttttaaa atcactgttt taaaggcaat aagataccga ccattcatat
    16321 ggaatggttt tattaactgc gtatgagtta aaacttatta gtggttcaga agatggtttg
    16381 cctcttgttt gatttgttgt agtacgttac gcacatgatt tatctgatta attgcatctt
    16441 ctttttcttt acgtaaccgc gcatccagta aatgattttc tggcaatggt gtttcactca
    16501 tctggcgata agggatccac ggattatcat cttcgtcttc aaagcggaac gccggcgcat
    16561 agtaatcaac ctcttcttcc aggccatatg ccgtcacctg cggctcatca tcacccaggc
    16621 agataaggtg cgttaataac gctttgaacg gcatatcacc ctgacctgaa atacaggctt
    16681 tatgacccag gccacccggt tctttaacga tcaaggcatc tttgatatgg acctgggtaa
    16741 tatgcggtgc catcgttttt aaagcgtcga tgggatgctc atttgcattg atcatgttcg
    16801 caaaatcaaa cagtaaggaa agagattcca tctcactttc tttgaccagc gacaccagct
    16861 catgactttt taaatcttca tgctgctcga tcgtaaaagt caggccgctg tcctgatacg
    16921 tttcccgtac ataggcaatg tcgttagcga taatcgataa tacgtcgcgc aaattacctt
    16981 cataacgtgg ataaaaacgt acggacgatg ccccagtttt caacgcgatg gcgacggctt
    17041 cgtcaataga tgccttatct gaggcgctgg tttcaatatg aatatcaagg ttcagacggc
    17101 gggctttatc cccaaaggca gagagttctt tatcgtccat attaccaaga gaaaaacgct
    17161 cgccatcgag aacatggatt ttgacgccac gtagattatt ttcacttgcg atatcaagta
    17221 aatcagcagg caacactctt tccagccgca tatttaaatg aaaggcataa gcatgtaaat
    17281 aaagaggaag agtgtcgaca cgttgtagaa tctttcttgc gttatttatt gtcaccataa
    17341 aacacctaca gattaatgat gaggtctacg gcctcaaatc gccaatattc aatatctatt
    17401 tggacaatat tgccttgctg gtcggcgcga tgcttttcaa ttaagatcgc tggcataccc
    17461 gcagaaccgc caagatactt gctgatatcg cccggcattc tgacgggttt gaaaatcaat
    17521 ttttttacta ccgtctcttt ttggtagcac ttttcccaga ctgcagaaaa tgagtggttt
    17581 tcaagttgtt caataaagcc cggagcaact tcaggattaa tatatgtttc gtgatagaaa
    17641 actttatgtc cttccagcgc cccccagccg gtgatgcgat agagttcagt tgatggcgtc
    17701 actgcaatca gtggcgcgag cgtctcggga atatcgctgg tacggttttt ctcggtaaac
    17761 ccccaggaag gctctcgccc ttgctcaatt gcggcccgct gaaagctcgc cgacagctcc
    17821 ggactgtaat taaatcgcgg ctgggtcacg aaccacccct tacgatcctt acggaagatt
    17881 ttggattctg cctcgaggtt caacaacgcc tggcgcagcg tcatacgttt aatgcccagc
    17941 agttctccca gctctctttc tgaagggagt tttccacccg gcgtggtgat gccatctttc
    18001 aaccaccgat ccagtttctc ttttgcattt tctacggtgg attgattctc cgccattgtt
    18061 taatcctttt ggtttaaacc agatgaagca tcttacgctt acgccaaaaa agaaagcaat
    18121 taacggcgta tgactttgca gataattgtg acaaagctct ctattgacgt aaccacagac
    18181 ttataaggga gatactcgca ttggtgtgcg ctcacgacag gcaaaaaaaa acctgccatc
    18241 gctggcaggt tttttatgac taaaaaacga aattaatcgt ctttcggtgc gcggttggcg
    18301 cggcgacgat cgttttccgt caggtgacgt ttacgaatac ggatagaggt cggagtcact
    18361 tctaccagtt cgtcatcatc gatgaactcc agagcttgtt ccagagtcat gcggataggc
    18421 ggaaccagaa caacggcttc gtcagtaccg gaagcacgca tgttggtcag tttcttaccg
    18481 gtcaggcagt ttacagtcag gtcgttagag cggctatgaa taccgataat ctgaccttcg
    18541 taaacttctg caccgtgacc gaggaacagc ttaccgcgat cctgcagacc gaacagcgcg
    18601 aacgcgaccg ctttaccctg accgttagag atcagtacgc cgttctgacg ctgacccact
    18661 tcacccggac gtacgtcgtc gtagtggctg aaggtggagt acagcagacc agtaccggaa
    18721 gtcatggtca tgaactcaga acggaagcca atcagaccac ggcttgggat cacgtagtcg
    18781 agacgtacgc ggcctttacc gtctggattc atgtttttca ggtcgccttt acgttcgccc
    18841 agcgcctgca ttacagaacc ctgatgctgt tcttcaacgt ccagagtcac gttttcatac
    18901 ggctcttgtt tacgaccgtc gatttcacgg aagataactt tcggacggga taccgccagt
    18961 tcgaaacctt cacgacgcat gttttcgatc agaacagaca ggtgcagttc gccacgacca
    19021 gaaacgcgga acgcatcggc gtcttcggtt tcttctacgc gcagcgcaac gttgtgtacc
    19081 agttctttgt tcagacgatc caggatctga cgagacgtta cgaacttacc ttctttaccg
    19141 cagaacggcg aggtgttaac gcagaagaac atagaaacgg tcggctcatc aacggagagt
    19201 gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt cagttcgcca
    19261 aggcccgtga tcgcaacgat atcgccagct tccgccagat cggtttcgat acgttccaga
    19321 ccgaggtggc ccagcacttt accgactttc gcgttgcggg ttttgccttc gctatcgatg
    19381 atagtgacct gctggttcgg cttcacttta ccgcgcttga tgcggccaat gccgataacg
    19441 ccaacatagc tgttgtaatc gagctgagaa atctgcatct ggaacggacc gtcaaggtca
    19501 acgtccggcg caggaacgtg gtcaacaatc gcctggtaca gcggggtcat gtcttccgcc
    19561 atatcttcgt ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac gatcgggaag
    19621 tccagctgct cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg atccacaacc
    19681 caatcaggac gcgcgccagg gcggtcaact ttgttgataa caacaatcgg cttcaggccg
    19741 taagcaaacg cttttttggt tacgaagcgc gtttgcggca tcgggccgtc aaatgcgtca
    19801 accaccagca gcactgagtc taccatggac attacacgtt caacttcacc accgaagtcg
    19861 gcgtgccccg gggtatcaac gatgttgata cggtaatcat tccatttgat agcggtgttt
    19921 ttcgcgagga tggtaatccc acgctctttc tccaaatcgt tggagtccat cacgcgctct
    19981 tgggtttcgg cacgagagtc gaacgtaccg gattgttgga gcagcttgtc taccagggtg
    20041 gttttaccat ggtctacgtg cgcgatgatg gcgatattac gcaatttttc gatcacaact
    20101 ttgcctcagg cattagaaat agcgcgttat tgtacacgga ttaatcgcac tacaaaacag
    20161 gatcacaaac atcctccgca aacaagtatt gcagagtccc tttgtgatcg ctttcacgga
    20221 gcataaaaag ggttatccaa aggtcattgc accaacatgg tgcttaatgt ttccattgaa
    20281 gcactatatt ggtgcaacat tcacatcgtg gtgcagccct tttgcacgat ggtgcgcatg
    20341 ataacgcctt ttaggggcaa tttaaaagtt ggcacagatt tcgctttatc ttttttacgg
    20401 cgacacggcc aaaataattg cagatttcgt taccacgacg accatgacca atccaggaga
    20461 gttaaagtat gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg
    20521 ttgatttgcg cttcaccgat actaaaggta aagaacagca cgtcactatc cctgctcatc
    20581 aggtgaatgc tgaattcttc gaagaaggca aaatgtttga cggctcctcg attggcggct
    20641 ggaaaggcat taacgagtcc gacatggtgc tgatgccaga cgcatccacc gcagtgattg
    20701 acccgttctt cgccgactcc accctgatta tccgttgcga catccttgaa cctggcaccc
    20761 tgcaaggcta tgaccgtgac ccgcgctcca ttgcgaagcg cgccgaagat tacctgcgtt
    20821 ccactggcat tgccgacacc gtactgttcg ggccagaacc tgaattcttc ctgttcgatg
    20881 acatccgttt cggatcatct atctccggtt cccacgttgc tatcgacgat atcgaaggcg
    20941 catggaactc ctccacccaa tacgaaggtg gtaacaaagg tcaccgtccg gcagtgaaag
    21001 gcggttactt cccggttcca ccggtagact cggctcagga tattcgttct gaaatgtgtc
    21061 tggtgatgga acagatgggt ctggtggttg aagcccatca ccacgaagta gcgactgctg
    21121 gtcagaacga agtggctacc cgcttcaata ccatgaccaa aaaagctgac gaaattcaga
    21181 tctacaaata tgttgtgcac aacgtagcgc accgcttcgg taaaaccgcg acctttatgc
    21241 caaaaccgat gttcggtgat aacggctccg gtatgcactg ccacatgtct ctgtctaaaa
    21301 acggcgttaa cctgttcgca ggcgacaaat acgcaggtct gtctgagcag gcgctgtact
    21361 acattggcgg cgtaatcaaa cacgctaaag cgattaacgc cctggcaaac ccgaccacca
    21421 actcttataa gcgtctggtc ccgggctatg aagcaccggt aatgctggct tactctgcgc
    21481 gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc tccgaaagca cgtcgtatcg
    21541 aagtacgttt cccggatccg gcagctaacc cgtacctgtg ctttgctgcc ctgctgatgg
    21601 ccggtcttga tggtatcaag aacaagatcc atccgggcga agccatggac aaaaacctgt
    21661 atgacctgcc gccagaagaa gcgaaagaga tcccacaggt tgcaggctct ctggaagaag
    21721 cactgaacga actggatctg gaccgcgagt tcctgaaagc cggtggcgtg ttcactgacg
    21781 aagcaattga tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg cgtatgactc
    21841 cgcatccggt agagtttgag ctgtactaca gcgtctaagt gttttagttg ccgtggaaac
    21901 ttttcgcctg tctctggcag gcctgggatc ggtggcaagc acatcacgcc ggatgcgacg
    21961 caaatgcgtc ttatccggcc tacacggtga tgatgtggta ggccggagca ggtgagtcgc
    22021 tctccaacgt gaagtttgtc agctatctgt agcccatctc tgcatgggct tttttctccg
    22081 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
    22141 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
    22201 tcaactcgct gattaacagt attttgttaa tcgatgacaa cctggcgatc cattacgcca
    22261 accctgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acaccgttac
    22321 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg
    22381 ggcaaggttt taccgataac gaagtgacgc tggtcatcga cgggcgctcg catatccttt
    22441 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggct ccgatggata
    22501 accagcgccg cttaagtcag gaacagctac agcacgccca gcaggttgct gcccgtgatt
    22561 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
    22621 agctgctcag caaagcgtta cctgacccat cactactcga atataccaaa gtgattatcg
    22681 aacaggcgga ccggctgcga aatctggtcg accgtctgtt ggggccgcag ctgcccggta
    22741 cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg
    22801 aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctaccg gaactggcgc
    22861 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
    22921 tggggccgga aggcggtgaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
    22981 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccgggcattc
    23041 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
    23101 ggcttggctt atccatcgct cgtaatttga ttgatcagca ttcaggcaaa attgaattta
    23161 ccagttggcc agggcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
    23221 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
    23281 tgaacgtgcg ctcgctgggg caggtttaac ctgtacgacg tttgagaacg gcgcagaagt
    23341 gctggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
    23401 aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc ttccggtcat
    23461 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
    23521 tgattatctg cccaaaccgt ttgatatcga cgaagcagtg gcgctggttg agcgcgctat
    23581 cagtcattac caggaacagc agcagccgcg taatgttcag cttaacggcc caacgaccga
    23641 tatcatcggc gaagcgccag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
    23701 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
    23761 tgccctgcat cgccacagtc cgcgcgccaa agcgccgttt atcgcgctga atatggcagc
    23821 tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg cgtttactgg
    23881 cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggtacat tattcctcga
    23941 cgaaattggt gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg
    24001 tcagttttac cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
    24061 cactcaccag aatctcgaac agcgagtgca ggaaggtaag ttccgtgagg atctgttcca
    24121 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc
    24181 ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt
    24241 actgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtgcgcca
    24301 gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag tgttgattca
    24361 ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
    24421 ggacagctgg gcgacgcttc ttgcgcagtg ggcagacaga gcgctgcgtt ccggtcatca
    24481 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
    24541 acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacaccct
    24601 gacgcgtaag ttaaaagagc tggggatgga gtgattcaca gcttgtgtgt aaagattgat
    24661 tattgagcgc aaattgctgg tattttacgc tttactgttc cgataagttc agtatgatct
    24721 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
    24781 cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcaatgat
    24841 tgggctgttt agctgaagct cgttgtttat gccggatgcg gagaaagtcg taggcctgat
    24901 aagacgcgac cagcgccgca tcaggcatca tgctcagatg cctgatgcgg cgtaaacgcc
    24961 ttatccggcc tacaaaagca ataatttcaa caggttttgt tgcccagcgc atcaggcaat
    25021 cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca
    25081 ctatttaaat cacccgagag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa
    25141 agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attcccttct
    25201 catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa
    25261 agtaatcagc gaagtgcaaa tcccactgtt tctcaatagg ggcgtaatcc agacggaagt
    25321 tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac
    25381 cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct
    25441 ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat
    25501 cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg
    25561 ccaccgccag ctcgtcatcc ggacgggcaa agtgatccat accgataaac tgatagcccg
    25621 attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca
    25681 ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa
    25741 agacactcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct
    25801 ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct
    25861 cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac
    25921 gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc
    25981 gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg
    26041 catcggcatt gaactggaag ttttcgcgca gcagcttcat caggcggctg atttgcgctt
    26101 tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga
    26161 acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt
    26221 gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg
    26281 ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg
    26341 cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc
    26401 cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc
    26461 tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt
    26521 catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa
    26581 ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc
    26641 aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc
    26701 ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag
    26761 tttagcatcc acccaggatt gctcttcggc actcagggtt tcgcctgctt ccagacgttc
    26821 cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag
    26881 cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca cgcccaatgg
    26941 aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga
    27001 cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg
    27061 cttgcgatca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg
    27121 acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt
    27181 attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga
    27241 ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg
    27301 ccgccaacac accctgtttt tcctttagcc ggaacaatcc gtgttgattc catttccgtt
    27361 taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc
    27421 ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta
    27481 cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta
    27541 tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg
    27601 ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg
    27661 ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac
    27721 tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg
    27781 cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt
    27841 ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctagctcgta
    27901 cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc
    27961 gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat
    28021 ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc
    28081 taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag
    28141 acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac
    28201 gtaaagcgca attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg
    28261 ttgaaacgtt ttcttcgttg aagaaacaag gcgtggacaa gctgcggcag aaactggata
    28321 cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc
    28381 ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac
    28441 cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa
    28501 aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga
    28561 agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc
    28621 ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt
    28681 tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa aaggcatgtt
    28741 caggcgaatc ttagtgcgcc tgatcccagt tttcgccact ccccacttcc accagcaacg
    28801 gcacatccag acgggtacag ttttccatca gttgatgaat ctgcttcgcg acggcatcaa
    28861 catcatcttt atgaacttca aataccagtt catcgtgtac ctgcatgatc atacgtacac
    28921 gcggttgctc agcctgtaac cacgcatcaa cggcaatcat cgcccgtttg ataatgtcgg
    28981 cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc agccgctgca cgacgagcac
    29041 cattgctgga tttgatatcc ggcagataca gacggcgtcc gtccagcgtt tcaacgtagc
    29101 cctgctcttt cgcctgagca cgggtgcgtt ccatatactc cagcacgcca gggtagcgtt
    29161 cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg aatgttcaat tgccgcgcca
    29221 gaccgaaagc actcatgcca taaatcagac caaagttgat cgctttcgcg ctacggcgtt
    29281 gctcgctggt gacggtttcc agtggcaaac caaacacttc tgccgccgtt gcccggtgga
    29341 tatcttttcc ttccgcgaat gcggtcagca agcctttgtc acgcgaaaga tgcgccataa
    29401 tgcgcagttc aatctgcgag tagtccgctg agacaatcac ataatcctct ggcgcaataa
    29461 acgcctggcg gatacgacga ccttcttcgt tacgcaccgg aatgttttgc aggttaggat
    29521 cggttgacga taaacgtccc gttgcagtta ctgcctggtg ataagaggta tgcacacgcc
    29581 cggttttcgg gttgatcatc agcggcagct tgtcggtgta ggtcgatttc agcttcgcca
    29641 gaccacgata ctccagaatc acttttggca acggatagtc cagcgccagt tcttccagta
    29701 cctcttccga cgttgacggc gcgccacccg gcgttttctt cagcggttta atgccctgtt
    29761 tttcaaagag aatggtttgt aactgcttgg tggaagaaag gttaaattcc tcacctgcaa
    29821 tttcatgcgc tttcttttcc agctcagcca gacgaagggt gagctcttca gaatgattgt
    29881 gcagcacttt cggatcgatc ttcacaccgt tacgttcaat gcgtgaaagc accggcacca
    29941 gcggcatttc gatattctcg aagacgttca acggcccttt gtgtttttgc agatccggcc
    30001 acattttcag atgcaactgc aaggtgacat ctgcatcttc ggcggcgtaa cgtccggctt
    30061 cttcgagggc aatctggtta aaggtcagtt gatttttgcc tttaccagca atctcttcaa
    30121 aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct gtccatatcg tgacgcccgg
    30181 caacgctatt gagaatgtag gactccagca tggtatcaaa cgcaatccca cgcagttcaa
    30241 tgccgtagtt cgccagaata ccgcgatcgt atttcaggtt ttgcccgacc ttcagcgcct
    30301 tttcatcttc cagcagcggt tttagcaact cgagtgcacg ctcgcgagag atttgatcgg
    30361 gcgcatcaag ataatcatga gcaaccggaa tatatgccgc tacgcctggc tcgatagcaa
    30421 aagaaagccc gaccaggtta gcagagatgt tatcaaggct gtcggtttcg gtatcaaatg
    30481 caaataccgg cgctttttcc agcttcgcaa tccacgcttt cagtgtttct tcatcaagga
    30541 tggtgacgta gttgtcataa gaaatcaccg ttgccgtcac ttctggtgct tcgtctgcaa
    30601 cactggtttc ctgtggcttc gcggctggtt ttgccccttt ggcctgtaac catttgcccg
    30661 cttcgacatc agcagtccag cgtttgaact catacttttt gaacagcccc aacaactctt
    30721 ccgctgccgg ttgctgcact tccagttgtt cacaggtcag ctccagttca acgtcggttt
    30781 taatcgtcgc cagctggtat gagagataag caacttcttt gttttgctcg agcttcgctg
    30841 ccattgtttt cgcgccacgg aagctcaacc cagcaatttt ttctggctcg gcatacagcg
    30901 tatccagtcc gccaagacct tgcagcaatg cctgcgcggt tttttcaccg acgcccggta
    30961 cgccaggaat gttatcagag gagtcaccca tcagcgccag gaaatcgatg atcagttctg
    31021 gcggcacgcc gtacttattc accacctctt ccggtccgag gatggtattc gtcatggtat
    31081 tgataagcgt aatatttggc gtcaccagct gcgccatatc tttatcgcca gtgctgatca
    31141 gcaccggacg cccggctttt tcggcttcgc gcgccagagt accgataacg tcgtccgctt
    31201 ctacgccaga aaccgccagc agcggcagtc ccatcgcttt aaccatcgcg tgcaagggtt
    31261 cgatttgtgc acgcagatcg tccggcattg gcgggcgatg tgatttgtaa tgttcaaaca
    31321 gttcatcacg aaaggttttt cccttggcgt caaagaccac cgctgcatgc gtcggtttat
    31381 attgcatgat cagactgcgc agcatgttga ggacaccata catcgcaccg gtcggctcgc
    31441 ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg ataaagataa gatgaaccat
    31501 ctacaaggat aagtggattt tgggggatct gaaccataat gtccgtgcct gtttatcaga
    31561 ttatgggtaa aggatgccac agacaggatg aaaacatgag tttttcaccg cttttcgtga
    31621 aaatcttttg tagatcttct ggatcgctcg caaatcgtca tgtggataac tttgtgcata
    31681 aattatttac atcgctttta atttattatc atgcttttta cagcatgatg tttgtctttg
    31741 tttttcatgc agttaaaaat aaatccatcc ctaataattg agataatggg attttttcta
    31801 atgtggatag atatgaatta tttttctcct taaggatcat ccgttatttg ggtcgttttc
    31861 tgctaaaatc tgcgccttgc gcttctttag cgcactgtct atggctaacc ttttgaataa
    31921 attcattatg acgagaatac tcgctgcgat aacccttttg ctgagtatcg tattgaccat
    31981 tttggtcact attttttgtt ccgtcccgat catcattgcc gggattgtaa aactattgct
    32041 acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt gattttatga tgtattgctg
    32101 gtgtgaaggt ctggcggtat tactgcatct gaacccacac cttcaatggg aagttcacgg
    32161 gctggaaggg ttaagtaaga agaactggta tctgcttatt tgtaaccacc gtagttgggc
    32221 agatattgtc gtactgtgcg tgctgtttcg taagcacatt ccgatgaata aatatttcct
    32281 caagcaacag ctggcctggg tgccatttct tggcctggcg tgctggtcgc tggatatgcc
    32341 gtttatgaag cgttattccc gcgcttattt attacgtcat ccggagcgac gtggtaaaga
    32401 tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg catcccacca ccattgtcaa
    32461 tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag caaacccact ccacttttca
    32521 aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg ctaaatgtac tgggtaaaca
    32581 attcgataaa ctgcttaatg ttacgctgtg ttatccggac aataaccgtc agccgttctt
    32641 cgatatgtta agcggtaaac tgacgcggat tgtcgtacat gtggatttac agcctattgc
    32701 cgatgagtta catggcgact acatcaatga caaaagcttt aagcgccatt ttcagcaatg
    32761 gctgaactca ttgtggcagg aaaaagaccg gcttctgacg tctctcatgt catcacagcg
    32821 tcagaataag taagttttac tcgcctctga atgagcagag gcgagtgagt attttaatga
    32881 acaaaacgtc cggcacgaga cataaattct tcttcgctca tctcctgtcc gttaaaaaca
    32941 actttacccg gtgtataacg cagctgcagc gaggcggtat tgttgtcaat cgtgattaac
    33001 tgaaacatct gacctaatgt catcatcccg ctgatttgtt tatcagcctg cttttgcgct
    33061 ttttccgcat ccattccttc agataaatta agctgttttg cggtttctgt gacgacatta
    33121 agcggtaact tcacatcaaa attgagcgat ttgatatctt tgtttgtgga tgatgaagat
    33181 ttggctgggt cggcaatact gatatccaga ttggcattta gttcgccgag tgcgttcttc
    33241 catcttaccg gttgtttaat ggtcggctca cttttttgta ataacggtaa atactctttg
    33301 aacaatgcag cattcacttc ttgcagagca acttcatcgt ttgctaactc agggtgtgca
    33361 gcaagctgct tctgcatcgc aatgttatac tggatgataa attggcgcac tgctgaggga
    33421 tcaatagatt cagcaatcaa tgaccactca ccactaccga gttgctgatt cccttttgtt
    33481 aacttatcaa gttcgtaggt gagattgaca ttgatataat ctttgtcttt aacgcgatcc
    33541 agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt cttccccgtg attgataatg
    33601 ttaatctgat taaatttcga ctcgctttca ccaaccggaa atttcttttc ttctaatcgc
    33661 gccagagaat caatggtaaa actttttgcc gttactttgt tcattggtga taattgcaga
    33721 acaatatctt cgactttgcc ttcaatatcc aggttagaca atgaactacc atcaccatta
    33781 aaagtaaact ggccctcacc aaaagaaaac tttccatact ctgcaggggc aacatctaat
    33841 gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg cttccacggg cgatttattt
    33901 ttcgcggcga taaatagcgg ttgtgtcagt tcattattca ctaagttcac ttttgccgct
    33961 gccagtgctg ggatgacatt accatgcatt aacattgtga tgggcaacgg accgtgctca
    34021 acatccacat caaatacaac tttctggcct ggcttgatat tgagatcggg tgcaccgtta
    34081 tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc ctttgtcaaa attttgataa
    34141 cttaacgtca tatcgtaggc atgttcacct ttctttttag catcgttaaa atctttaata
    34201 aatttttcga caccaggctg aatttgcgta cctgtgtacc atgtgccacc accccagatt
    34261 acggctaacg caacaataac acctgtagct gacttacgta tcatataaat attccctgat
    34321 aaaacagcaa aactttgaat atccacttat gctgaaataa tgtccag
//
LOCUS       DBBGLQ010000047        32679 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.047, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000047 DBBGLQ010000000
VERSION     DBBGLQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.047"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            121..639
                     /locus_tag="ACIYSB_002955"
     CDS             121..639
                     /locus_tag="ACIYSB_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGC5429964.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            655..1194
                     /locus_tag="ACIYSB_002956"
     CDS             655..1194
                     /locus_tag="ACIYSB_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGC5429965.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1287..2864)
                     /gene="guaA"
                     /locus_tag="ACIYSB_002957"
     CDS             complement(1287..2864)
                     /gene="guaA"
                     /locus_tag="ACIYSB_002957"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGC5429966.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFITVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2933..4399)
                     /gene="guaB"
                     /locus_tag="ACIYSB_002958"
     CDS             complement(2933..4399)
                     /gene="guaB"
                     /locus_tag="ACIYSB_002958"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGC5429967.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4561..5931
                     /gene="xseA"
                     /locus_tag="ACIYSB_002959"
     CDS             4561..5931
                     /gene="xseA"
                     /locus_tag="ACIYSB_002959"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGC5429968.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPAAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFTSRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5928..6143)
                     /locus_tag="ACIYSB_002960"
     CDS             complement(5928..6143)
                     /locus_tag="ACIYSB_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGC5429969.1"
                     /translation="MELHCPQCQHVLDQDNGHARCRSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6213..7685)
                     /gene="der"
                     /locus_tag="ACIYSB_002961"
     CDS             complement(6213..7685)
                     /gene="der"
                     /locus_tag="ACIYSB_002961"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGC5429970.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7803..8981)
                     /gene="bamB"
                     /locus_tag="ACIYSB_002962"
     CDS             complement(7803..8981)
                     /gene="bamB"
                     /locus_tag="ACIYSB_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGC5429971.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8992..9612)
                     /gene="yfgM"
                     /locus_tag="ACIYSB_002963"
     CDS             complement(8992..9612)
                     /gene="yfgM"
                     /locus_tag="ACIYSB_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGC5429972.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9630..10904)
                     /gene="hisS"
                     /locus_tag="ACIYSB_002964"
     CDS             complement(9630..10904)
                     /gene="hisS"
                     /locus_tag="ACIYSB_002964"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGC5429973.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11015..12133)
                     /gene="ispG"
                     /locus_tag="ACIYSB_002965"
                     /gene_synonym="gcpE"
     CDS             complement(11015..12133)
                     /gene="ispG"
                     /locus_tag="ACIYSB_002965"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGC5429974.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12160..13173)
                     /gene="rodZ"
                     /locus_tag="ACIYSB_002966"
     CDS             complement(12160..13173)
                     /gene="rodZ"
                     /locus_tag="ACIYSB_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGC5429975.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13458..14612)
                     /locus_tag="ACIYSB_002967"
     CDS             complement(13458..14612)
                     /locus_tag="ACIYSB_002967"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGC5429976.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14762..15193)
                     /gene="ndk"
                     /locus_tag="ACIYSB_002968"
     CDS             complement(14762..15193)
                     /gene="ndk"
                     /locus_tag="ACIYSB_002968"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGC5429977.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15342..17654)
                     /gene="pbpC"
                     /locus_tag="ACIYSB_002969"
     CDS             complement(15342..17654)
                     /gene="pbpC"
                     /locus_tag="ACIYSB_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGC5429978.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDFTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKITTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NSIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEQPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17655..22616)
                     /gene="yfhM"
                     /locus_tag="ACIYSB_002970"
     CDS             complement(17655..22616)
                     /gene="yfhM"
                     /locus_tag="ACIYSB_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGC5429979.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVVQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSSLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEQLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22823..23668
                     /gene="sseA"
                     /locus_tag="ACIYSB_002971"
     CDS             22823..23668
                     /gene="sseA"
                     /locus_tag="ACIYSB_002971"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGC5429980.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            24330..24455
                     /gene="timP"
                     /locus_tag="ACIYSB_002972"
     CDS             24330..24455
                     /gene="timP"
                     /locus_tag="ACIYSB_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGC5429981.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24486..25262)
                     /gene="sseB"
                     /locus_tag="ACIYSB_002973"
     CDS             complement(24486..25262)
                     /gene="sseB"
                     /locus_tag="ACIYSB_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGC5429982.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25404..26687)
                     /gene="pepB"
                     /locus_tag="ACIYSB_002974"
     CDS             complement(25404..26687)
                     /gene="pepB"
                     /locus_tag="ACIYSB_002974"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGC5429983.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVVWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26865..27065)
                     /gene="iscX"
                     /locus_tag="ACIYSB_002975"
     CDS             complement(26865..27065)
                     /gene="iscX"
                     /locus_tag="ACIYSB_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGC5429984.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(27077..27412)
                     /gene="fdx"
                     /locus_tag="ACIYSB_002976"
     CDS             complement(27077..27412)
                     /gene="fdx"
                     /locus_tag="ACIYSB_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGC5429985.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27414..29264)
                     /gene="hscA"
                     /locus_tag="ACIYSB_002977"
     CDS             complement(27414..29264)
                     /gene="hscA"
                     /locus_tag="ACIYSB_002977"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGC5429986.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29281..29796)
                     /gene="hscB"
                     /locus_tag="ACIYSB_002978"
     CDS             complement(29281..29796)
                     /gene="hscB"
                     /locus_tag="ACIYSB_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGC5429987.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29892..30215)
                     /gene="iscA"
                     /locus_tag="ACIYSB_002979"
     CDS             complement(29892..30215)
                     /gene="iscA"
                     /locus_tag="ACIYSB_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGC5429988.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30232..30618)
                     /gene="iscU"
                     /locus_tag="ACIYSB_002980"
     CDS             complement(30232..30618)
                     /gene="iscU"
                     /locus_tag="ACIYSB_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGC5429989.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30646..31860)
                     /gene="iscS"
                     /locus_tag="ACIYSB_002981"
     CDS             complement(30646..31860)
                     /gene="iscS"
                     /locus_tag="ACIYSB_002981"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGC5429990.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31972..32460)
                     /gene="iscR"
                     /locus_tag="ACIYSB_002982"
     CDS             complement(31972..32460)
                     /gene="iscR"
                     /locus_tag="ACIYSB_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGC5429991.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
BASE COUNT         7685 a         9065 c         8130 g         7799 t
ORIGIN      
        1 aaattattcc ctgcgtgaat tttaataaat ttaatctatc cctttatacg caatacattt
       61 actttcctct tttgatgatc ttaaatgtct tatttttcgt aatgtgtata acaaggaata
      121 gtgatgaaat ttaaaaaatg tcttctgcct gtggcaatgt tagcgtcatt cactctggca
      181 ggatgccagt caaatgctga cgatcatgct gccgatgttt atcaaaccga tcaactgaat
      241 accaaacaag aaactaaaac cgttaatatt atttccattc ttcccgcaaa agttgccgta
      301 gacaactccc aaaataaacg gaacgcacaa gccttcggcg cgcttattgg cgcagtcgct
      361 ggcggtgtta tcggccacaa cgtcgggtct ggcagcaatt ccggaacgac ggcaggtgca
      421 gttggcggcg gagctgtagg cgcggcagcg ggttctatgg tgaatgataa aaccttagtg
      481 gaaggtgttt ctttaaccta taaggaaggc accaaagtgt atacctctac ccaggtgggt
      541 aaagagtgcc agtttacgac aggtttagcc gttgttatta ccacgacgta taacgaaacg
      601 cgtattcagc caaataccaa atgtcctgaa aagagctaat aatcaggagg agtcatgaag
      661 aaagtttttc tttgcgccat cttagcctcc ttaagctatc cggctatcgc ctcatcattg
      721 caggatcaac tctctgctgt cgcagaagcg gaacagcaag gtaaaaatga agagcaaagg
      781 cagcatgacg aatgggtcgc ggagcgcaac agggaaatcc agcaagagaa gcaacgtcgc
      841 gcaaatgccc aggccgccgc taacaaaaga gcggcaacgg cagcggcaaa taagaaagct
      901 cgtcaggata aactggacgc cgaagcctct gcggacaaaa aacgcgatca aagttatgaa
      961 gatgagctac gcagcttaga gattcagaaa caaaaactgg cgctggcgaa agaagaagcc
     1021 cgcgttaagc gagaaaacga atttatcgat caggaactga agcacaaagc tgcgcaaacc
     1081 gatgtggtgc aatctgaagc tgacgccaac agaaatatga ctgaaggcgg tcgcgatctg
     1141 atgaaaagcg tgggcaaagc agaagagaac aaatcggaca gctggtttaa ttaatcgatg
     1201 ttagtaactt caatcctata attcttgaag ataaaaaacc ctctgtagta acagagggtt
     1261 ttgttcattc atagtgcagg gtcaaatcat tcccactcaa tggtagctgg cggcttgccg
     1321 ctgatgtcat acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg
     1381 cgaccgagga aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc gatggtttcg
     1441 acagcacgca gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa
     1501 cgtaccggca ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc
     1561 agttcttcaa tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact
     1621 tcacccagaa cacgaacgcc aaggcctggt cccgggaacg ggtgacggta cagcatgtcg
     1681 tacggcaggc ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc
     1741 ggttcaacca ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg
     1801 atgacgtgtg ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg
     1861 ccctgcgcca gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg
     1921 aaaacgcgac cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac
     1981 aggaagcgat cttctgccgg tacgtgaaca atgttaagac caaagtgatc gccaaacata
     2041 tccagaacct gctctgcttc gttgaggcgc agcaggccgt tgtcgacgaa tacgcaagtc
     2101 aggtttttac cgatagcgcg gtgcagcagc attgcggtta cggaggaatc cacaccacca
     2161 gagaggccga ggatgacttt atcgtcgcct acctgctcgc ggatgcgagc tacagcatcg
     2221 tcgataattt tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc
     2281 tccagcatgc gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag
     2341 aagcgttttt cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg
     2401 atgaagtcgg acggaatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc
     2461 ggtttaccgt ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct gtcgtttacg
     2521 acttcaacct gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac
     2581 tgcattgcca tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag
     2641 acatactgcg gcgcacgcgg actgttttct tcagtagtac tttccgggcc gccggaaaga
     2701 ataatgccgc ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt
     2761 tcgcagtaaa cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg
     2821 aagtccagaa tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag
     2881 gcaagtgaaa cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc
     2941 ccagacggta gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa
     3001 tgcccgcacc gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac
     3061 agccggtcag acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca
     3121 ggcgaccttt ataggctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt
     3181 tatcgctctg gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac
     3241 cacggtaaga tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag
     3301 tacccgccag catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga
     3361 tgtcgccgga gaagcgaata ccgccatcag cgataaccgg aataccggtg ccttccaggg
     3421 cttctactgc gtcagcaaca gcggtaatct gcggaacacc gacgccagtc acgatacgag
     3481 ttgtacagat agagccaggg ccaatgccga ctttaaccgc actgcaacca gcttctgcca
     3541 gagcgcgtgc acctgcagct gttgccacgt tgccgccgat aatttgcaga tccggatatt
     3601 tagcacgggt ttcacggata cgttgcagta caccttctga gtgaccgtgg gaggagtcga
     3661 tcagcagaac gtcaacgcct gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac
     3721 ctgcgccaac cgctgcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt
     3781 tacgttccgc tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat
     3841 caaccaccag cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg
     3901 cttcaccttc acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct
     3961 ggttcaggtc ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt
     4021 cttcggtaac gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc
     4081 gcagcgtcgt ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt
     4141 tcacacggcg aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga
     4201 taccgccttc ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg
     4261 cggaaagcat agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag
     4321 tattcggcag aacggtagag tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt
     4381 ctttagcgat acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg
     4441 gcattataca gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc
     4501 ccgaagggcg ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac
     4561 atgttacctt ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt
     4621 ctgctgcttg agcatgagat gggacaggtt tggatcagcg gcgaaatttc taatttcacg
     4681 caaccagctt ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc
     4741 gcgatgttcc gcaacagcaa ccgccgggtg accttccgcc cacagcatgg gcaacaagtt
     4801 ttagttcgcg ccaatattac gctctacgag ccgcgcggcg actaccagat aatcgttgag
     4861 agtatgcagc cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag
     4921 ttgcaggctg aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat
     4981 tgcgttggtg tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta
     5041 aaacgtcgcg atccttctct gccggtgatc atctaccctg ccgccgttca gggcgatgac
     5101 gcgccggggc aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgta
     5161 ttgatcgtcg ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc
     5221 gtagcgcggg cgatttttac cagccgcatt ccggttgtca gcgccgtcgg gcatgagacg
     5281 gatgtgacca ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct
     5341 gaagtagtga gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg
     5401 ctggagatgg cgatggatta ttatctcgcc aaccgcacac gtcgctttac gcaaattcat
     5461 caccgattac agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag
     5521 cgcctgcaaa agcgaatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag
     5581 cagcagcggt taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg
     5641 caaacgcgca ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgc acagcttagc
     5701 gccacgcgtg aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca
     5761 acgctggcgc gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg
     5821 aagcaagtta aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt
     5881 gaagtaaaaa acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg
     5941 aattcaacgc gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca
     6001 ccacaggctt ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca
     6061 ataaattccc cgcaggatcg gcaacgcgca tggccgttat cctgatcaag cacatgctga
     6121 cattgtggac aatgcagttc catatgaatt cctccggtag ccattccctc tacattcata
     6181 gagggaatgg cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc
     6241 agacgcttac gtttacgcat ctgggttggc gtcagggtgt tacgcttatt cgcatacggg
     6301 ttttcccctt ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg
     6361 aagtagttca tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga
     6421 atcaccacaa tcggcgggtt ataaccaccg gcgtgggcat atttcagctt cacacgacga
     6481 ccgcgtacca gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata
     6541 gaggtcccca cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta
     6601 ccaacaccac tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc
     6661 agacggaagt ccagcgtttc tttcacctgc tctttcactt cctgactcag gccatcccac
     6721 ttattcacca caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga
     6781 tcctgatcgg aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggcgtcttca
     6841 atggcctgca acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt
     6901 ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattgggatg
     6961 tagatgctgt cacgcgtcgt gccaggcatg tcgtaaacaa caacgcgctc ttcaccaaga
     7021 atacggttag tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc
     7081 ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca
     7141 aattgcgccc agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc
     7201 cacggcagca gcacatgctc cagcagactt aatacgccac gaccgtgaga cgcggcgatc
     7261 gggtagattt cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga
     7321 ccgtcagttt tgtttgccac caggaaggtc ggtttttcac gggagcgcag atgtttggca
     7381 atcgcttcat ctgccggcat caggcccgcg cgcgcatcca ccataaacag tacgacgtcc
     7441 gcttcttcaa tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg
     7501 ccatcaatcc cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg
     7561 tacttacggt cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt
     7621 agacggttaa ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt
     7681 accatgttta aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt
     7741 tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac
     7801 gattaacgtg taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca
     7861 gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga
     7921 ccatcttcga cgttaatcca gtgcagataa ccttcactgt caccgaccac caggttgcca
     7981 ttatacagca ccggagaagt cagcaggcga tgcagcagat cgctttgtgt ccacagcgta
     8041 acgccgccat caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga
     8101 ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca
     8161 ctgcgcagat caagcgccgt caggttacca ttataggcca gcgcgaaaac aacgccgtta
     8221 acaacgacgg gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc
     8281 tgggaaatac gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga
     8341 ccattatcgc cccccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc
     8401 aaagagagcg aaggcatatc gaggttaact gtccatttga cagcgccgtc agcttcgttc
     8461 agcgcttgta actgaccgtt actggtgtgg attaacacca gaccgtcgct gaccaccggg
     8521 cgcgaaagtg cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc
     8581 agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaca
     8641 ccgccagaaa gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca
     8701 gaccagattt ctttgccatc atccgcattc agcgctttta ctaaaccagc gcggtccgct
     8761 gcatagacaa cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg
     8821 ctaccaacgg aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat
     8881 ggggacatct ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta
     8941 acggaaagca gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga
     9001 caaattatta attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac
     9061 gcctgcttcc catgcactac gcgcaccttg cttatcacct ttgctcagca atgcttcacc
     9121 acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag
     9181 cgcggcatca gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc
     9241 tttgagattt tcatcgctcg tgtctgccag cccctgttgt aactgggcgg cagctttctc
     9301 tagttcattt ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt
     9361 atttttattt tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct
     9421 cactgcggta accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg
     9481 gctgttccag tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag
     9541 tgctttgcca ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc
     9601 gtaaatttcc acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct
     9661 acgctatcct gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg
     9721 ccgttagcca cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca
     9781 cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt
     9841 aattcatcac gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa
     9901 gccaccaggt atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact
     9961 aacaatacaa gacgttcgag gcccatcgca aaaccgacag ccggtgttgc acgaccgccc
    10021 agttgttcca caagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgaga
    10081 ctgttagtca cccactcgaa aacggtacgg ttgtagtaat ccagaccacg caccagacgc
    10141 tggtttacgg tgtaagcgat ccccgcgctc tccagcagtt tgcacagacc ggcaaaatgc
    10201 tcacgagatt cctcgtccag atagtcacct aatgccggag cgtcgttgag aagcgcctgc
    10261 acttccggat tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag
    10321 tcttcgtcca gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg
    10381 gcgcgtgctt ccagcgaacc gatagagttc agctcaagag ttacgtgctc ggaaataccc
    10441 agcgcgcgcc accagcgggc agtgagcata atcagttcag cgtcgatatc cggaccttgc
    10501 agaccgaaaa cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc
    10561 tcgtgacgga acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca
    10621 tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg
    10681 ccattgcgat cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc
    10741 gcacgtttga atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag
    10801 ctgccgagca cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc
    10861 aggtaatcgt tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg
    10921 aatataaaaa tgaaccctca acgcttccct caatgtttcg ggagccatgc gggttcaatc
    10981 atacacggga agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa
    11041 ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga
    11101 tcatatcgtt gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct
    11161 tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc
    11221 agccgataat cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt
    11281 taaccgtacc gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttga
    11341 tccctcgcga acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga
    11401 ccggatcggc cgccagcgat acgcgcagcg tgtcgccgat gccttcagac agcagcagac
    11461 ctaaaccaat ggcggatttt actgccccgc tgcgcgcacc accggcttcg gtgatcccca
    11521 gatgcaacgg ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga
    11581 cgtcagacgc tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa
    11641 catgacgcat ggcagattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca
    11701 gatctttttc cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg
    11761 cacagtcaac caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca
    11821 gacaatcgac gccgtattcc gctactttca gcgcaatgcg atagtcgaag tggatgtcag
    11881 ccaccagcgg cacgttaacc tgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg
    11941 tcggtacgga tacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga
    12001 ccgttgcttc gacgtctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac
    12061 catcgccaat cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag
    12121 cctggttatg catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc
    12181 ggcattgagg gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg
    12241 tttcccttga tactggatct gtactgcggc tggcgcacca attttcagtt tgtacggtgc
    12301 ctggccggtt aagttcaaat taccgtcttt acgctgcata ccgctaaaca attttttacc
    12361 ggtagcatca gtgacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg
    12421 atcagccacc ggcgtggtca cgccagcctg atcggttggc aacggcgcag caccatctgg
    12481 cgttgttgct gccgttggtg ccggggtcgc ggcggtatca acatttgcct gcgaaggcga
    12541 aacaaccgca ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg
    12601 cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt
    12661 agttgtcgac gtatttaatg gaacactctg cccctgctcg ctattactgc tcagttccgc
    12721 cgaagattga tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca
    12781 ccaggcaccg ctcaggccga taaccacaaa caacaccagc caagtgaagg tcatcagcca
    12841 gccgtcgcgt tttttgcggc gtttaccgag ggaaaaactc tgcatcggcg caacttttgc
    12901 agcccgaagt ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac
    12961 cagacgcgca taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc
    13021 cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc
    13081 ctgctgacta agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc
    13141 ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat
    13201 tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt
    13261 tatcatacag tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa
    13321 cttactgttg catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga
    13381 ccagacaatt gcaacttaat acttcattca taaaacatta cgccacggta cataaagtaa
    13441 ccgtggcgta atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta
    13501 cgcagggtac gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatcg
    13561 atatcatcac cacgagtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt
    13621 gagaaacggt cgatacggct gttcgagctg cgtccatacg gcgcacccgg gaacgggttc
    13681 cacgggatca ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg
    13741 tgttcagtgc cgtcgttaac gtggtcaagc atcacgtatt caatagtgac tcggccctga
    13801 ttggcgttgg atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac
    13861 tttttgttga tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt
    13921 gccagtgcaa cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagtg
    13981 gaaagcgtga cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc
    14041 gccggaacga cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta
    14101 gtgatcggac gctgaccggt gacttttgct gcgccgacga ttttcgccgc acgccacacc
    14161 tggccgataa tttccgacac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag
    14221 aatttacact ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct
    14281 tccgggatat acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg
    14341 ccgtcagatg aacgctgttc ttcaaccact tccggtgcgc ggatttccgc cacctctttc
    14401 agtttgccgc gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag
    14461 tgatacatcc acttcatcac ctgatcggcg cggaaggttt tttcacctaa atctttaaaa
    14521 aactcccgca tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg
    14581 acgttttcag gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt
    14641 tatggcccct ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc
    14701 gctgcattgt acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt
    14761 attaacgggt gcgcgggcac acttcgcctt cgccaaagaa ataagcgatt tcgcgagcgg
    14821 cagattcgac ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag
    14881 cgcgcagagt accagccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac
    14941 gctgaacggc gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt
    15001 caaccagacc atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgtgcctgtt
    15061 caacggtcag gtgcagcatt ttggtgccaa caattttgaa ccctgcagct tcaaagcgcg
    15121 caaagatatt accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac
    15181 gttcaatagc catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag
    15241 atggcgcgga ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt
    15301 aaaataaaat gagttttagc aacaatcagg gacaaaacag actattgcat gacaaatttc
    15361 actgtcgcga tttgccccac gtcatccatc accagcaatt gataatcacc tttatccgtc
    15421 aaatgcaggg tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag
    15481 cgttcacctg cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa
    15541 cgtttaataa tcgcgccatc gcgaacgcct gtcagctgca gcgggagctg ggcatcgtga
    15601 ccgtatggcg ggcaacttgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg
    15661 ggcagccagg gttccagcgg tagcggccag acatttatca tttcttgtcg cgcttgcggg
    15721 caatcagcgg caacacgttt gccattttca tccagccaga tggggaaacg aatgccatta
    15781 atgccttcct gctccggcag taatagagtt ggcggctgac tcccgtccag cagccaggtt
    15841 gccaggcggc ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag
    15901 ataacgccac gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac
    15961 agtaagatat tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca
    16021 acaacgggcg tgccgtccgg tctgccagtc cagatcccaa tgacatagcg agcgttaacc
    16081 ccaatcgccc aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg
    16141 acgcgcggca aggcactatc cggcaagggt tgcgcttcat cagccataat ccggcgaatg
    16201 atccacgccg cccccgacga cattaaaggc tgttcaagca gcggatcgtc aggctgtaag
    16261 cgcaatttgc ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc
    16321 agttttgcac cagcgccgcc gagaatgagt gaaagattcg gcgcagcacc gttgggcaaa
    16381 tataacggca atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc
    16441 agcacctgca cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg
    16501 ccatgaaaac cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct
    16561 tgcagcagtg atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt
    16621 ttgagcactg atcctggcga tcggatcgaa ttgaccatat caacatgacc aaagcgtgaa
    16681 tcatcgttga gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc
    16741 acgatcatcg ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct
    16801 tccagacgtc gttgaagacc ggcatccaac gtagtagtga ttttgtcgct tttgctttta
    16861 ccgagcatca tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg ggccagccag
    16921 atgggttctt cccttgactc ttttacctgc tcacgggacc acacaccttg cacggccatc
    16981 cgttcgagca ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga
    17041 cggctgggcg cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca
    17101 ggcgattttc cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac
    17161 ggagcgcggt taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc
    17221 aacgcgcgcc agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga
    17281 cgagcaacct gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgaaatct
    17341 tgccatgctg cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga
    17401 tcttcataat tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga
    17461 taacgccaga tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg
    17521 accactcgtg cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac
    17581 gctgcaagaa aaaggagaaa gggcgcggct gccaacgtta tccagcagcc gcgtttggtt
    17641 aacaagcgag gcatttacgg tctgacaatc agcagatctt cagccgcgcc ggtcgcccgc
    17701 cattggggaa catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc
    17761 accgcccgcg ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc
    17821 acaaagcgat cgtcacggaa ctcaatgtgc ttaatgctcg cctgctgcat ctggttcagt
    17881 aagttctgca cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctgg
    17941 ttttccagtt ccagacccgc aggcagcaga tccacgacta acgcatccgg cacgctgtta
    18001 ctggctttta cctgcaacca caccagcacc agatcgccgc tacgtaacga gtccagcgat
    18061 ttgctcttac catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt
    18121 aaaggtgcgg attgcggata accgctggca tccatacgca accataacgg ctgatcgcca
    18181 ctgttgctca cctgcaaggt gacaagttga tcgctattca gattgctgtt ttgcgctttc
    18241 tcgcctgtca gctgctcagc tgagaaagag gtttgcgcct gccatttacc gggtaaatcc
    18301 tgaatcgtac gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca
    18361 ccaaacgcct gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg tagcagttta
    18421 ttttcttcca gcaaggagag cattaacgcg ttgtcgcgca gtgaactacc gtaatcaccc
    18481 agccatatcc gctcatcact attacgcggc gttttcagcg ccagcgcaat cgcttcttca
    18541 ccacgcgtcg catcacccat ggttttcagc gcaacgccaa gttgcagcag cggtaaacca
    18601 gaagcggcat ctgcacgatg ctcccagatt tcacgcagcg cacccagcgg agccttttgc
    18661 tgacgggcca acaccagcgc agcgtaagac tgtacggcga atttactggc tttgagatta
    18721 tccgcgtacg ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta
    18781 ccccggttaa tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa
    18841 tccatcacgt aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag
    18901 ccgccgttat cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg
    18961 cgtttctcat cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa
    19021 agtgacggaa acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac
    19081 tctttgatat aacgtgcgat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc
    19141 agcgtaacag gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc
    19201 tgtaacgccg taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc
    19261 cactgcttat gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc
    19321 tgaatttctc catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc
    19381 acacctggcg ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg
    19441 gcggtcaggg caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc
    19501 agacgcgagg tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt
    19561 gcggcaacta tcactttact ttcgttgcta ccgaagtcat ctgccgtcca ggcttgcgcc
    19621 atgacgcgca attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt
    19681 tcgttgagcg ttaccggcag cgcctgctgc acgacaatat tgacgtgatt gaccggcggt
    19741 ttaccaccac gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc
    19801 tgaccttcaa taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca
    19861 aagaacgcct gccacggatc tggcgtgacg tagtcagtaa tattcagcac accactatca
    19921 acggcagaca ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta
    19981 attttcacgg ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg
    20041 aggcgacggt tttcatcgcc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc
    20101 gcggagcgag atttatcgcc aggacgtacc accagcgtac ttaaatacag atcatgacga
    20161 ttccaggttt tatcgaccgg aatcgtcaga tccagccctt gagcccgaac atcaatctct
    20221 tgccaccaca gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc
    20281 gcggcgatat gcaacttaat ggtgtcgcca gggcgataac tggctttatc cagtttcagc
    20341 gtgacacggt cgggtcgcac tgcgccgcta ccgtcgctgt tgtcctgcca gctatagcca
    20401 gcccagaaac gaacactact gaccgcttca ttcggcgctt tgacttccag acgataagcg
    20461 ccccactcta ccggaaaact gaccttgccg gtttcgtccg ctttcagatc cagagtttgt
    20521 tcattttcga tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag
    20581 ttccagtagt aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt
    20641 ttcacgcctt gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca
    20701 atgggctgtt tcaccgtgct gtcagtacga taatcgtaaa ccgatttcga ggcgaactgc
    20761 ggacggatcc ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc
    20821 gggcgaccgc ccgattccag caaactaccc tggaaaataa cctgtaatgg ggaatgcgtt
    20881 tccttccact ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga
    20941 acttcatcca gcgtgcggga aagattttcg gcagctatat cgccgaattc aaaaccaggt
    21001 aaggctgaca cagcttcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca
    21061 ttagcaggtg caccatacag gtagtacccc accacggaga atttcacttc atctttcggc
    21121 gttagcgggg ttttctcacc ggtcagattc agcgccatgc gctctggcat aaaatcttcg
    21181 acgtggaaat cccacatccg atactgatta tcgcccgtgt tagcgcgaat atgccacata
    21241 ccggttgccg cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga
    21301 ctaacgacgc tcctgagtac ctgcccatcg ggtttaatca cgtctaactt gatgggttga
    21361 ttgggcaacg ctttaccgtc tgcatcacgc agcaaaccat tgaggattac cgtttcacct
    21421 gggcgataaa gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca
    21481 gcaatgttaa attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga
    21541 ccgtctttac gcgccaacag taatgccgcg tttttatcat tttccagctg cacatgcccc
    21601 tgagcgtcac tggttgcctg agtcagagtc tgccctttct catttaataa agagacttca
    21661 attccttgct gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga
    21721 taacggtgag ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa
    21781 tcgtaacgtc cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg
    21841 atatcgccca gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac
    21901 cgtccggtgt agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc
    21961 gaattgcggt attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag
    22021 aagttaacat cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg
    22081 ccaggcagca gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt
    22141 atagtttttt cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca
    22201 atagtaacga tcaaatcacg tttcggttcg aggtggcgta aacgtaactc tttaagatta
    22261 tctgacagct cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg
    22321 cgtgagaaat cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca
    22381 ccatcaagtt ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg
    22441 gccagttttt gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc
    22501 gctttagtaa cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg
    22561 tcgcaccctg ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt
    22621 catccctgcc acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg
    22681 ttatgtaatt cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac
    22741 agccaaatca gcctcttttg cttgtcgctt ttcactaaac atccgacaat ttagcctaac
    22801 cccggcaaaa atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc
    22861 cgaacatatt gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca
    22921 ggaggatcgt aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt
    22981 tgatatcgaa gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga
    23041 aacgttcgcc gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta
    23101 tgacgaaggt aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt
    23161 agagaaagtg tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt
    23221 agaagaaggt gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc
    23281 cgtggtgaaa gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga
    23341 tgcccgcccg gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg
    23401 cggacatatt cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact
    23461 aaaaacgacc gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc
    23521 aattatcgtc agctgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac
    23581 gctggatgtg ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc
    23641 agatttaccg gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt
    23701 tggcggtttt ttattgctag tctggttcgc ggcctttcca gcaggttgac ttgtgttaca
    23761 tgagcaacgc aggtgcttca cagcaaaaca atactcacca gtaactctct ttttgtcaag
    23821 caaaagagag taattattgt ttatttagcg tattatcgac accggccctt tccgccgtgt
    23881 tcggtaataa aataacctgg cttattagtc cgaattcaga caaatataaa caaatcctgc
    23941 tcaaaattaa aaattctaac cggtaaaaga tattacttaa acatgtaaat tcactttcct
    24001 ttaaaaaaca aaaaaccgcc aaaatcaggc ggttttttgt tgctggtccg gttcgcggcc
    24061 tttccagcag gttgtattac cgtagtaatg caagcgcgtc tcagcggaga caatactcgc
    24121 cagtaactct ctttttgtca agcaaaagag agttattatt gttctgttag tgtattatcc
    24181 actgcggccc tttccgccgt ctcgcaaacg ggcgctggct ttaggaaagg atgttccgtg
    24241 gccgtaaatg caggtgtttc acagcgcttg ctatcgcggc aatatcgcca gtggtgctgt
    24301 cgtgatgcgg tcttcgcatg gaccgcacaa tgaagatacg gtgcttttgt atcgtactta
    24361 ttgtttctgg tgcgctgtta accgaggtaa ataataaccg gagtctctcc ggcgacaatt
    24421 tactggtggt taacaacctt cagagcagca agtaagcccg aatgccgccc tttgggcggc
    24481 atattttaga ttatccgatt ctgtttaaag tcacgcaaaa aaccacccca gcgacgttca
    24541 tagaatggcg caatatgttc ggtaataaag tggctaattc ctttttcccc ttttttcacc
    24601 tgacaaatat cgattggttc atcgccaggt aatgtatcgg tcgctacact tcccgtcgcc
    24661 tgaataattt cttcgatatc accatcggct tcaatgccaa taagtaaatt aggctgtgcc
    24721 tcttcgttct ctttaattga acaaataaaa gcacgcttca ccggcttaat ggttttaaat
    24781 aaggtggtga gtgaatcaat catttgtgct ggcggctctg cgacttccga taatatcagc
    24841 gattcaccgc cttccaggat ttcctggctg ctcagcggat ttccctcttc accaatcaac
    24901 aaactgattt cacgcggcat aaattcttta ccggttggca gtttggcatt aaggaagagc
    24961 gtttcgccaa gtgtcatctc aaacagcgtg cgaacgggca ttacgacaaa tgcctgttcg
    25021 tcttcaaccg cctgttgaag tgcttctaac gaggtgaaaa aaggaatgac gctggtgccg
    25081 tcttcttttt cccagtgctg taaatcaagc gcgctatctt caaccacagc ctcgccctgc
    25141 gccgccgtac caggcaccca gacggtggat tccagtagag tacggaaaaa ggccgggcgg
    25201 tgcgccggtt cagttgctgc tttttccagc aggtcttcaa gttcgttttt tgtttcggac
    25261 ataagaacca caattcattc aacgttcggg gcgcaaatgc ctgatgcgct acgcttatca
    25321 ggcctacaag ggattcgcaa tttgttgaat ttgcagaatt tgtaggccgg ataaggcgtt
    25381 tacgccgcat ccggcatatt agtttacgcc gttaacagat tagctatcgt gcgcacacca
    25441 agtcccgtag cgcccgcaga ccactgttca accggcgctt tacggtaagt cgccgagcag
    25501 tcgatatgca gccagccttg ctgatagttc tcaacaaagt gcgacaggaa gcccgccgcc
    25561 gtgctcgcgc ctgccggata cgccgcgctt ccggtattgt tcagttcggc aaagttagac
    25621 ggcagctggc tgcggtggaa ctccgccagc ggcagacgcc agaacggttc gttctcctgc
    25681 gcggcactcg ccagcaagcg accggccagc gcatcgtcaa aactgaacag cgcgtgataa
    25741 tcattaccca gcgcagtttt cgccgccccg gtgagggtcg ccgcatcaat gatcatttcc
    25801 ggtttctgcg cactggcatc aatcagacca tcggcaagca ccagacgccc ttcggcatca
    25861 gtgttcatca cttcaacttt tttaccgttg cgataggtga tgatatcgcc cagcttgaac
    25921 gcattgccgc taatcaggtt atccgcacag cagaggaaca gcttcacgcg cttgttcagt
    25981 ccgcgcgtaa tggcaaatgc cagcgccccg gtaaccgttg ccgcgccgcc catgtccgac
    26041 ttcatcgagt ccataaacgc agtctgtttg atgctgtagc cgccggagtc aaaagtgata
    26101 cctttaccta ccaggcacgc gtacactggc gcttctttat cgccagttgg gttgtaatcc
    26161 agcgccagca ataccggaga acgttctgaa ccgcgtccga ctgtgtgcag ccccatataa
    26221 ccttgctcac gcagatcttc gcctttggtg atccgataag tcacacgatc gcccgcgacg
    26281 ttgctgatca gatcaacagc acgctgtgcc agttgcgatg gtcccaattc ttctgccggt
    26341 gcgttgatgg tgtcacgcac ccagtcgatg atcatcaggc ggttatccag ttcctggcgc
    26401 tgggcatcgt ccagatccgg ccacaccact ttacgcgtgc ctttcggggc tttgtaacct
    26461 tgccagaatg cccagcagcg atccgcatcc cagccttcac cgcttaactg aacatgcttg
    26521 atgcccagac cgtcaatctt gcgcgccgca cgctggatca gccccagatc gtctgccccg
    26581 ttcaaatgca gggtaatgcc gtcattatta atgctgtaag ttgctttttc tccccagcgc
    26641 gcgtcggcag gttgggtaga gagggtaatc ttcatcgctt ctgtcatttt agttatcctt
    26701 ctttgtaaaa gggccgccag ttggcagccc caggattttt ccgctaaatg attcgcgttg
    26761 caggaaggcg gcaagtgagt gaagccccag gagcatagat aactatgtga ctggggtgaa
    26821 cgagcgcagc caacgcatct gcggcgtgaa gcatgacgcg gaaattattc ggcctcgtcc
    26881 agccagacta acaaaatcgc ttcgaggatt ttctcgttgg atgcctgcgg gtcgtcgtcg
    26941 aaatcttcca gatcgcaaat ccactgatgc atatcggtga atcgaaccgt tttcggatca
    27001 agatcgggat acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt ccacttaagt
    27061 cccatactaa cctctgttaa tgctcacgcg catggttgat agtgtaacgc gggatttcga
    27121 ctactaaatc ttcgtcggta acgcgcgcct ggcagcttaa acggctttcc ggctccagtc
    27181 cccaggcttt gtccagcatg tcgtcttcct gctctgagct ttccggcagt gagtcaaaac
    27241 cttcacgaac gatgcagtgg caggtggtgc aagcacagga tttttcacag gcgtgttcaa
    27301 tctcgatacc gttacgcaga gctgcgtcga gaatggtttc accgctatta gcttccagaa
    27361 cagcgccatc agggcagaga tcctgatgag gcaaaataac aatctttggc atattaaacc
    27421 tcgtccacgg aatggccttt cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg
    27481 aaatcctggg tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc aacatcatcg
    27541 ccctgcgcca cttcactcag gtgagcggca gcatcgtcaa tgacctgacg ttctgcggcg
    27601 cttaacagcg cggcatcagc agccagcgcg ccgtgcagac tttccagcac acgcgccgct
    27661 tctacttttt gttctgccag cattcgggct tttacgtctt gctcggcata gctcattgag
    27721 tctttgatca tcgaagcgat ttcgctatcg gtcagaccgt aagacggttt gacctgaata
    27781 gacgcctcaa cgccggtgga tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg
    27841 acctggaacg tcacgcgaat atgcgcaccg ccagccggta gcgccggaat accacgcagc
    27901 gcaaaacgcg ccagtgagcg gcagtcctgc accagttcgc gctcaccctg cattacatgg
    27961 atagacatcg ccgtctgacc atctttaaag gtggtgaaat cctgagcgcg ggccaccgga
    28021 atagtggtat tacgcggaat cactttctcc accaggccgc ccatcgtttc gaggcccagc
    28081 gacagtggga tcacatcaag caacagcatt tcgctgtctg gcttgttacc caccagaata
    28141 tccgcctgaa tcgccgcgcc aatagcgacg actttatccg ggtcgatgga agtcagcggt
    28201 ggacgaccga aaaattcgcc tacccgttca cgcaccagcg gcacgcgagt agaaccgccc
    28261 accatcacca cttccagcac ttcatcagct tctacacccg cgtctttcag cgcgcgacga
    28321 caagccagta aggttcgttt taccagtggc gcgatcagtt cattgaattg ttcacggctg
    28381 atttcgccct gccagcccgc aacgttaacg gtcacggagt ccgcatcgct cagcgcgatt
    28441 ttggctgcaa tggcggcatc cagcagttca cgctgaacgc ggttatcgct acgatcagga
    28501 atgcccgcct gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc gccgccgagc
    28561 gcggaatcac cgccggttgc cagcacttca aacacgccgc gacttaagcg cagaatggaa
    28621 atatcaaacg tcccgccacc gaggtcataa acggcgatca cgccttcctg accggaatcc
    28681 agcccgtagg cgatagccgc agcggtcggt tcgttaagta agcgcaggac gtgaaggccc
    28741 gccagacgcg ccgcgtcttt ggtgccctga cgctgggcat cgtcaaagta cgccggaacg
    28801 gtgataacta caccatccag ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct
    28861 ttgaggatgt ccgcagaaac gcgcaccggg ttcagcagcc ccgccgccgt ttcaatcatc
    28921 ggcaggccgt tttcgctggc ctggaattga taaggcagat gcggatagcg ttgctggata
    28981 tcagccagcg agcgtcccat caggcgttta acagaactaa ttgtgttggc ggtatcgagc
    29041 gctgcattag tacgcgcgtc ataacccacc gaatgccctt gctgttgata gtgaacaaca
    29101 gatggcagca ggtgacggcc ttcatgatcg gctaacgttt cggcctgacc gctgcgcact
    29161 gtcgccacca gcgagttggt tgtgcccagg tcaataccgg ccgccagacg acgctgatgc
    29221 ggcgcagcac tcaaaccagg ttcactaatt tgtaataagg ccatgtttag cttccagaaa
    29281 ttaaaaatcg agcagttttt cttcgagttg ttcggcactg cttcgcagtt tatcgagaaa
    29341 acgcagctta cgcacggtat ccgccgccgc gtcccacgtc tcgttgtcta actgttcaac
    29401 catcaactga tggcgggtat caaacatctt tttcacacgt ttgataaagc tttccagccg
    29461 cgcttcatct ttcgcctgtt cgatctcgtc cagctcttcg cgcagctcca actgttccat
    29521 caggaacgcg gtgtcgcgca cagtatgctg ctcgctggcg agatcaaagc cgtgcaaaga
    29581 aagcaaatat tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct ggttaatggt
    29641 tgcagattgc tgtacggcgg cgagttgttc cgcctggctt ccgctggcga atttatcagg
    29701 atgatactga cgttgtagat cctgaaaacg caggctcagc gcctgggtat cgagttgata
    29761 acgggcaggc aagccaaaga gggtgaagta atccataaca ttctcagggc tgcgatccgc
    29821 cgcgataacg gcgaatcagg taaaacaaac cccacgcgca ggcgaccacg gtggggttat
    29881 cggtatgcgc atcaaacgtg gaagctttcg ccgcaaccac actcatcttt gacgttcggg
    29941 ttggtgaatt taaacccttc gttcaggcct tcttttacga agtccagctg cgtaccgtcc
    30001 agaaattgca ggcttttgcc atcgaccacg actttcacgc ctttgtcttc aaacacgatg
    30061 tcttccggcg tcggttcgtc aacaaattcc agtacataag ccatacctga acatccggag
    30121 gttctcacgc ccagacgcag gccaaaccct ttgccgcggt tagccagaaa ggtatttact
    30181 cgcgctgctg cactgtcgct cagtgtaatc gacataacca aacctcaact cttattttgc
    30241 ttcacgtttg cttttatagt ccgcaatggc ggctttgatc gcgtcttctg ccagaataga
    30301 acagtgaatt ttcaccggcg gcagttcaag ttcttcagca atatcggtat ttttgatcgc
    30361 ctgcgcttcg tcgagagact tccctttcac ccattcggtg accagggagc tggaagcgat
    30421 agcagaaccg cagccgtaag ttttaaaacg cgcgtcttca atgatacctt catcgttgac
    30481 tttaatctgc aacttcatca cgtcgccaca ggccggtgcc cccaccatgc cgctgccgac
    30541 gttctcgtcg ttgttgtcaa aggaacccac gttacgcgga ttctcgtaat ggtcgataac
    30601 tttttcgctg taagccatta taaattctcc tgattccgat accgattaat gatgagccca
    30661 ttcgatgctg ttcagatcca cgccctgctt gtacatttcc cacagcggag aaaggtcacg
    30721 cagacgaccg atggatttac gaactaactc gatggtgtag tcgatctctt cttcagtagt
    30781 aaaacgacct aaagagaaac ggatagagct atgtgccagc tcgtcgttca gccccagcgc
    30841 gcgcagcacg taggacggtt cgaggcttgc tgacgtacag gcggaacctg aagaaactgc
    30901 gaggtctttc agcgccataa tcagcgactc accttcaacg tagttgaagc tgacgttgag
    30961 aatgttcggc gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga tatctttgat
    31021 gccgttccac agacggttac gcaggccgcg cagacgttcc atctcggtcg ccatctcttc
    31081 ttttgcgatg cgataggcct cgcccattcc gacgatctgg tgaacaggca gagtgccgga
    31141 acgcataccg cgctcgtgac cgccgccgtg catttgcgct tcgatgcgta cgcgcggttt
    31201 acgacgtaca tacagcgcac cgataccttt cgggccatag attttgtgac cggagaaaga
    31261 catcaggtca actttcaact ggctcaggtc gataggcagt ttgcccacgc tctgggttgc
    31321 atcaacgtga tagataatgc cacgagcacg gcacatttcg ccgatagccg cgatatcctg
    31381 caccacgccg atttcgttat ttacgtgcat gatggaaaca aggatggtgt cgtcacgcat
    31441 cgctgcttca agttctttca ggtcgataat gccgttacgc tgcggtgcca ggtaggtgac
    31501 ttcaaaacct tcgcgctcca gctgacggca ggtatccagt accgctttgt gttcggtttt
    31561 gctggtgatg atgtgcttgc cttttttctg ataaaagttg gctgcacctt tgatcgccag
    31621 gttgtcagat tcggttgcac cagaggtaaa gacgatttca cgcggatcag cgccgaccag
    31681 atcggcaatc tgattacggg cgatatctac cgcttcttca gcctgccagc cgaaacggtg
    31741 agaacgagag gccgggttac caaaggttcc gtccatcgtc atgaactgca tcattttctc
    31801 ggcaacacgc gggtccaccg gcgtggttgc ggagtagtcg agataaatcg gtaatttcat
    31861 tgctctataa actccgtaca tcactcaatg caaggaatca ggctaccggc tggatgtacg
    31921 accgtgttta cggagtattt agcactccgg cctgattctg aattcttttt attaagcgcg
    31981 taacttaacg tcgatcgcgt cttgtgtgcg ggtgcgtggc gcgtcgtgag tatgctgacg
    32041 accagacaca tccagcactt cctggttatt aaccagttcg cctaaagtaa tgttgttgag
    32101 aaaaccggtg agacggtcgc tcaaatcacg ccacagcgcg tgggtcaggc atttatcgcc
    32161 gccctggcag ccgcctttac cctgacaacg ggtggcatct acagattcgt caacggcgct
    32221 aattacttcg ccaacggcga tgctgctggc atctttgcct aacagataac caccgcctgg
    32281 tccacgtacg ctggaaacca gaccattttt acgcagacgg gaaaacagtt gttccagata
    32341 agaaagggaa attccctgac gttcggaaat atcagccaac ggtaccgggc ccgcttcaga
    32401 gttgagcgca acgtcaagca ttgcggtcac ggcatagcgc cctttagatg tcagtctcat
    32461 gtcttacttc acctcaaact cgcccctgcc cggggttttt tattgtaaaa gtgggggtat
    32521 tgcatagcag ggtcaagtct gacattcccg agtaaattgg tcaactattt acttgactga
    32581 tttagtcggg tatttaacct tcagtgccat ttttttatcg tggcgtttgt acctgttgtc
    32641 ggatgcggcg tgaacgccat atccgccctg gggttctgt
//
LOCUS       DBBGLQ010000048        30787 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.048, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000048 DBBGLQ010000000
VERSION     DBBGLQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.048"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            216..716
                     /gene="tssB"
                     /locus_tag="ACIYSB_002983"
                     /pseudo
     CDS             216..716
                     /gene="tssB"
                     /locus_tag="ACIYSB_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            751..975
                     /locus_tag="ACIYSB_002984"
     CDS             751..975
                     /locus_tag="ACIYSB_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5429992.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1026..2501
                     /gene="tssC"
                     /locus_tag="ACIYSB_002985"
     CDS             1026..2501
                     /gene="tssC"
                     /locus_tag="ACIYSB_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGC5429993.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLVVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAYAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2508..2921
                     /gene="tssE"
                     /locus_tag="ACIYSB_002986"
     CDS             2508..2921
                     /gene="tssE"
                     /locus_tag="ACIYSB_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGC5429994.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQVVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2925..4775
                     /gene="tssF"
                     /locus_tag="ACIYSB_002987"
     CDS             2925..4775
                     /gene="tssF"
                     /locus_tag="ACIYSB_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGC5429995.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4739..5820
                     /gene="tssG"
                     /locus_tag="ACIYSB_002988"
                     /pseudo
     CDS             4739..5820
                     /gene="tssG"
                     /locus_tag="ACIYSB_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            5845..7125
                     /gene="tagH"
                     /locus_tag="ACIYSB_002989"
     CDS             5845..7125
                     /gene="tagH"
                     /locus_tag="ACIYSB_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGC5429996.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7122..7646
                     /gene="tssJ"
                     /locus_tag="ACIYSB_002990"
     CDS             7122..7646
                     /gene="tssJ"
                     /locus_tag="ACIYSB_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGC5429997.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7649..8980
                     /gene="tssK"
                     /locus_tag="ACIYSB_002991"
     CDS             7649..8980
                     /gene="tssK"
                     /locus_tag="ACIYSB_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGC5429998.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISNRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8985..9746
                     /gene="icmH"
                     /locus_tag="ACIYSB_002992"
     CDS             8985..9746
                     /gene="icmH"
                     /locus_tag="ACIYSB_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGC5429999.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9755..12520
                     /gene="tssH"
                     /locus_tag="ACIYSB_002993"
                     /gene_synonym="clpV"
     CDS             9755..12520
                     /gene="tssH"
                     /locus_tag="ACIYSB_002993"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGC5430000.1"
                     /translation="MIQIDLPPLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            12517..13260
                     /gene="vasI"
                     /locus_tag="ACIYSB_002994"
     CDS             12517..13260
                     /gene="vasI"
                     /locus_tag="ACIYSB_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGC5430001.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAIQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13265..14677
                     /gene="tssA"
                     /locus_tag="ACIYSB_002995"
     CDS             13265..14677
                     /gene="tssA"
                     /locus_tag="ACIYSB_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGC5430002.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATEQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14786..18220
                     /gene="tssM"
                     /locus_tag="ACIYSB_002996"
     CDS             14786..18220
                     /gene="tssM"
                     /locus_tag="ACIYSB_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGC5430003.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRYLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRTSLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18231..19680
                     /locus_tag="ACIYSB_002997"
                     /pseudo
     CDS             18231..19680
                     /locus_tag="ACIYSB_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19607..20089
                     /locus_tag="ACIYSB_002998"
     CDS             19607..20089
                     /locus_tag="ACIYSB_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGC5430004.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20133..21048
                     /locus_tag="ACIYSB_002999"
                     /pseudo
     CDS             20133..21048
                     /locus_tag="ACIYSB_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21058..21537
                     /gene="ykfM"
                     /locus_tag="ACIYSB_003000"
     CDS             21058..21537
                     /gene="ykfM"
                     /locus_tag="ACIYSB_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HGC5430005.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNTNYHPCGNNTGIFPGAIYIKNGMKCNNGYISRKEDSAVK"
     gene            complement(21674..22459)
                     /locus_tag="ACIYSB_003001"
     CDS             complement(21674..22459)
                     /locus_tag="ACIYSB_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430006.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNDKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(22787..22863)
                     /locus_tag="ACIYSB_003002"
     tRNA            complement(22787..22863)
                     /locus_tag="ACIYSB_003002"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22827..22829),aa:Asp,seq:gtc)
     gene            complement(22996..23727)
                     /gene="dnaQ"
                     /locus_tag="ACIYSB_003003"
     CDS             complement(22996..23727)
                     /gene="dnaQ"
                     /locus_tag="ACIYSB_003003"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGC5430007.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEEIAAHEARLDLVQKKGGSCLWRA"
     gene            23792..24259
                     /gene="rnhA"
                     /locus_tag="ACIYSB_003004"
     CDS             23792..24259
                     /gene="rnhA"
                     /locus_tag="ACIYSB_003004"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGC5430008.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(24256..24978)
                     /gene="yafS"
                     /locus_tag="ACIYSB_003005"
     CDS             complement(24256..24978)
                     /gene="yafS"
                     /locus_tag="ACIYSB_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGC5430009.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGKLYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISFMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            25012..25767
                     /gene="gloB"
                     /locus_tag="ACIYSB_003006"
     CDS             25012..25767
                     /gene="gloB"
                     /locus_tag="ACIYSB_003006"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGC5430010.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            25839..27197
                     /gene="mltD"
                     /locus_tag="ACIYSB_003007"
     CDS             25839..27197
                     /gene="mltD"
                     /locus_tag="ACIYSB_003007"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGC5430011.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(27245..27868)
                     /gene="yafE"
                     /locus_tag="ACIYSB_003008"
     CDS             complement(27245..27868)
                     /gene="yafE"
                     /locus_tag="ACIYSB_003008"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGC5430012.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVAR
                     MRTPEALVDAIRIYQQSASTEVRTYFALQNDGFFTSDIIMVDAHKAA"
     gene            complement(27872..28672)
                     /gene="yafD"
                     /locus_tag="ACIYSB_003009"
     CDS             complement(27872..28672)
                     /gene="yafD"
                     /locus_tag="ACIYSB_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGC5430013.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            28913..29827
                     /gene="yafC"
                     /locus_tag="ACIYSB_003010"
     CDS             28913..29827
                     /gene="yafC"
                     /locus_tag="ACIYSB_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGC5430014.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(29824..30627)
                     /gene="dkgB"
                     /locus_tag="ACIYSB_003011"
     CDS             complement(29824..30627)
                     /gene="dkgB"
                     /locus_tag="ACIYSB_003011"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGC5430015.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         7693 a         7929 c         8008 g         7157 t
ORIGIN      
        1 cacaggttca tacaatctat ttaacgcttg cagaataccc ctctttacga tcgataaaaa
       61 gtccttttct tatttatttt gaagcgtaaa atgcggggtg atgaaggagt ggtgatttta
      121 ttttcaccgc catcaaggcg gcatttccga tattttttac ggtacgaata tgccgtgttc
      181 atggcctgta cgagataaca tgacggatca cttttatgag caaaaaattt gaaggcagcg
      241 tcgcgcctcg cgaacgtatt aatatatcgt atgtcccaaa aacagatggc cagacggctg
      301 aagtggaact tccgcttaac atgctcgttg tcggcgatac cggaaatacg caggaaacat
      361 cctcactgga tgaacgtcag gcagtttccg ttaacaagca caatttcggt gcagtgatgg
      421 ctgaagcagc cattggcctc aattttaccg tccccgccac gctgaaggga agcacaacgg
      481 acgatgagct gaatgtggcg ctgaatatca agtaactgga tgatttttca ccagatagcg
      541 tggcccggca ggttccggaa gtgaataaat tgctggaact gcgtgaagca ctgactgcac
      601 tgaaggggcc gatgggtaac cttccggcat tccgtaccca gctccaggca ttgctggaaa
      661 atgaagagtc ccgcgagcag ttgctcaaag aaatcggcca ggtgagcaat aaataaccac
      721 cactatttca ttaaacaggg ttatccaggc atgacggcac acaaaaatat ctccgggaca
      781 taccacctct ctgttgcaga cattctgcag gtggtttacc aggtatgttt cagcccgtcc
      841 gttgaaatca accaggatgg tgtggctgcg ctgattacaa cactggatcg ccgtatcagt
      901 gatctattgg atgagattat ccatttttgt gagtttcagc agagcgcgtc gcactggcag
      961 cgcgtacttc actgatggcg aaaagccggt ttcagaaaca cagctaaaac agaaggattt
     1021 tcagtatgtc tttacaggaa gaggaactcg tttccagtca tgccgggcag cctgagcagg
     1081 catcgtcgct gcttgatcaa atcatggcac agacccgtat tcagcccggt tcggaaggtt
     1141 atgacgttgc ccgccagggt gtgacagcct ttattgccag catcctgcaa tccactgcat
     1201 ctgccgaacc ggtcaataaa ctggtcgtgg acagcatgat tgcagatatt gatgaacgca
     1261 tcagccgcca gatggatgtc atcatccatg cgcctgcctt tcagcaggtc gagtcgttct
     1321 ggcgctcact gaaaaccatg gtggatcgcg ttgatttccg tgaaaacatc aaggtcaacg
     1381 tgcttcatgt caccaagcag gaacttctgg aagactttga gtttgcgcca gaaattattc
     1441 agtccggatt ttataagcac gtttattcgt ccggtttcgg acagtttggc ggtgaaccca
     1501 tcgctgccgt gctgggcgct tatgagttca aaaataccgc gcctgacatg aaactgctgc
     1561 aatacgtcag tgcagtgggc gcaatggcgt acgcaccgtt cctgtcctcc gtttccccgg
     1621 agtttatggg gctgaactcg tggaccgaac tgcctaatat caaagatctt tatgccatct
     1681 ttgaaggtcc ggcctacacc aaatggcgcg ccctgcgtga ctcggaagat tcgcgctatt
     1741 tagggcttac ggcaccccgc ttcctgcttc gccagcccta ttcaccaaca gataaccctg
     1801 ttaagaactt taactattac gaagatgtca gccagaacca cgaagattat ctgtggggta
     1861 atacggcctg gatgctggca tgcaatatcg cagacagttt tgccaaatac cgctggtgtc
     1921 caaacattat cggtccgcaa agcggcggcg cagtgaaaga tctgccggtg catctgttcg
     1981 aaacgatggg gcagattcag gccaaaatcc caaccgaggt gctggtcacc gatcgccgcg
     2041 aatttgaact ggctgaagag ggttttatca cccttaccat gcgtaaagac tctgataatg
     2101 cagccttttt ctctgcaaac tcggtacaaa aaccgaagca ctttccggga aaagatgcag
     2161 aaaccaacta taagctgggt acgcagcttc cttatctctt catcatcaat cggttagcgc
     2221 actacatcaa ggtgttgcag cgtgaacaac tggggtcatg gaaagaaagg agcgacttag
     2281 aaagagagct taatacctgg atccgtcagt atgttgccga ccaggaaaac ccgcctgcag
     2341 acgtgcgcag ccgcaaacct ctgcgcgctg ccaaagttga ggttatggat gtagaaggcg
     2401 aacccggctg gtaccaggtt gcgctaagcg tgagacctca ttttaaattt atgggggcaa
     2461 attttgagct ttccctggtt ggccggttag acagggagta acacaccatg ataccggatc
     2521 gggaaagaac cacaggttct ctttttgagc gtatggaagc ctcttcggcc agaaaccggc
     2581 agggaggcag tattcattcg ctcaggcaat ccattcgcca gaacctacga aacattctta
     2641 atacccggtc cggtagttgc cggggtgcgc ctgagctggg cattgatgaa ccggaagggg
     2701 ctgaaaactt ccgcgaatcc atgagtcggg ccatcgagca atgtatagaa cgctatgaac
     2761 cccggatctc acacgctgaa gtgcaggtag tggtttcatc cgcctccagc ccgctggata
     2821 tgacgtttca tattacagcc tgggtgacat ttaacgaaac gcatgaggtg cttgaatttg
     2881 atatggcacc gaatggcagc cagcattacc gggtggactg acaaatggaa tttgaagaac
     2941 gctatttccg ggaagaactc gattacctgc gccagcttag caagctgctg gcaacggaaa
     3001 aaccccatct ggcccgcttc ctggccgaaa aagatgcgga tccggatatt gaacgcctgc
     3061 tggaaggggt ggcttttctt accggcaatc tccgccagaa aattgaggat gaattcccag
     3121 aactgacgca cgggcttatt aagatgctat ggcctaatta cctgcgtccg gttccggcaa
     3181 tgacccttat tgaatatacg ccggatatgg ataagtcttc tgtaccggtg ttaatccccc
     3241 gtaatgagca gtttacaacc aacgccgggg aaatcagagt tgatgaagtg ctgccctctg
     3301 atgctaaaaa ggaggagccg cctccctgta ccttcaccct ctgccgggat atctggctgc
     3361 tgcccgttcg cctggagcag attgaaaacc gcagtacgac ccgtaatggt gttatcaaca
     3421 tcaccttttc ggtcgcaccg ggaacggact tccgcacgct ggatctgaac aaacttcgct
     3481 tctggctcgg caatgacgac aactataccc gtgaccagct ttatttatgg ttctgcgaat
     3541 acttgcaggg tgccgacctg actgtgggtg aacagcatct tcgcctgcct gagtttatgc
     3601 taaaagctgt cggttttgag ccgcaggatg ccatgctgcc ctggccgaaa aacgtccaca
     3661 gcggctaccg gatccttcag gagtatttct gttaccccga tgcgtttctc ttttttgatc
     3721 tttgtggttg tccggctttg cctgacggat tgcaggcgga attctttacc ctgcaactgc
     3781 gtttttcgcg ccctttgccc gtggacatcc ggctgcgccg cgattccctg cgcctgtatt
     3841 gcgcacctgc cattaattta tttatccacc atgcagaagc catcacgctg gacaaccggc
     3901 gggcagacta tccgctggtt cccagccgcc attacccaca acattacgat gtattttccg
     3961 ttaacagtgt ggtgagccag gtccaggata tgttcaggaa aaaagatctg gggcgtcctg
     4021 tttcgacgca ggccgcgcgc cagtggccag cctttgaaag tttcagccat cagatggaat
     4081 acagccggaa gcgggaagtg gtgtactggc atcaccggac caaaacatcc ctgttccatc
     4141 gcggctttga tcataccctt gcctttatac atgctgatgg cagttatccg tcagacgaat
     4201 ctctgctcag taatgaagtg gtttcggtat cgctgacctg taccaaccgt gagcttccgt
     4261 cacaaattcg ttccggcgat atcaccggca caaccggtaa aaatgcagct gttgcttcat
     4321 ttcgcaacat tacccgcccg acgcaaccac tctggccggt cattgatggc agcctgcact
     4381 ggtccctact ctccgccatg aacctgaatt atctgtcatt actggatacg gacgcgctga
     4441 agcaggtcat cgccaacttt gatcgccacg caatccatca tccgcagacg gcacggctgt
     4501 cacaacaaaa gctggatgcc attgagcgtc tggagacccg ccccgttgat cgcctgttta
     4561 cgggtattcc cgtccgggga ctggcctcca cgctgtatct gcacccggag ccgtttgtct
     4621 gtgaagggga aatgtatctg ctcggtacgg tgctttcgca ttttctgtcg ctgtacgcca
     4681 gcgttaactc attccacatg ctgaccgttg tgaacacaga aagccaggag acatggaaat
     4741 ggacggaaag aatcgggcag catcctctta tctgagtccg ggaaatcccc cggcagataa
     4801 agagcagaat gatccgctgg cacaggtttt ccataatgcc tgctcttaca atttttttgc
     4861 gatggcggag ctgttgcacc gcctggcaaa gggtgaaaag gggacgccag aattatccct
     4921 gcgtgacgat ccggcacagg aaaccctgcg tttcagcgcc gatgccagcc ttgccttccc
     4981 gtgcagtgat atcagtgcgc tgaaacggga tacatcgggg cattcaggat gaccaccacc
     5041 tttatggggt tacaggggag ccagtcgcct ctccccggtt attacctcga tcacctggcc
     5101 tggaaagcgg tacatgaaca aagtccggta ggtgattttc tggatatgtt cagccaccgc
     5161 ctgacccagt ttgtctggca tatctggcgt aagtaccgct atcacatcag ttttcgtaac
     5221 ggcggtgtgg atgcgttctc gcagcgcatg tactcccttg tcgggctggg ccaccgccag
     5281 cttcgcgata agctggcgat taatcacagc aaaatgctgg cgtattccgg gatcctggca
     5341 aatccgggac gatccccgga aataatctgt ggcctggtgt cccactgctt tgatttgtca
     5401 gaagtgacgc tgcaaaactg gcaacgtcgc aaggtggata ttgaaccgga tcagcaaaat
     5461 agtctgggga gctattctct taaaaatggc gaaaaactgg cgggaagatc ggtactgggc
     5521 aactttgttc tgggtacacg agtgcccgat ctcagcggaa aattccagct cagtattacc
     5581 agcctgaccc gaaaacagtt cctctctttt ctgccgtccg gcgaaaactt cctgccactg
     5641 acgatgttcg tgtccttcat cctgcgcgat cagcttgcct gggacttaca tctggggctg
     5701 gcgccggagc aggtgggcgc aatgcgcctg ggtgataaca aaagtgcgct gctgggctgg
     5761 accagcttcc tcggcacacc ggaagaacga ccctcagtca ccatcagggt acggtcgtaa
     5821 attatgataa tcacggagca gaatatgccg gaagagaaac tacagacgct ttcattgcag
     5881 gtcattaacg gcagtgagct ggaaagcggg cgggcggcgc gctgtctatt cacacagcag
     5941 ggaaatgtcg gccatggccc cgaatgccac tggtcggtac aggatcgtca gcagagcatt
     6001 ccggcccagg cttttaccgt tatcctgcac gatggcacat tttgtctgcg cccgcagacg
     6061 gcacaactgt ggctgaatca ggcaaaagtc acagcaacat cagacctaat acagttgcgc
     6121 cagggcgatg agatccagat cggacggctg atggtgaggg ttcatctgaa ccggggagat
     6181 attccccatt acgatgagga aatggccact cccgaaacca tcgttaccaa tcgcgatatg
     6241 ctcacggata ccctgctatc aacggagggt gcgccacact atccgggaat gactcaccgg
     6301 caccagcttg cagacaccgt ggtaaacggt ttttctgccg atccactcca ggcacttcag
     6361 tccgaaagcc tgattaccac gggcgatccg ctttcaggca ttgcggctgt ccggccatcg
     6421 gcaccgctgt ccgatccggc aagtaatggg gggatcaata ctccgtttat ggatctgccg
     6481 cccatttatg ccagccccgg cgatcgtaat gacgacgtct ctgcggcaga aatggcgcaa
     6541 cgccaccttg cggtcacccc cttactgcgc ggtcttggcg gctcgcttac catgagcaat
     6601 tccgacgatg cggatgattt tctggaggag gccggacgaa cgttacaggc cgcaataaaa
     6661 ggtctgctcg atttgcagca gcagcgtaac agcctctcag acaaacattt gcgcccgctg
     6721 gaagataacc cgctgcgcct gaacatggat tacgccaccg cgctcgacgt aatgtttgcc
     6781 gaaggtaaaa gcccggtaca tctggcagct cccgccgccg tcagtgaaag cctgcgcaat
     6841 gtccgccacc acgaagaagc taaccgggca gcgattgtgg agtcgcttcg tgtcctgctg
     6901 gatgctttct caccacaaaa tctgctgcgc cgctttgtgc agtaccgccg cagccatgaa
     6961 ctgcgccagc cgctggatga tgccggagca tggcaaatgt acagccatta ttacgaagaa
     7021 ctggcctccg atcgccagca ggggtttgag atgctgttta acgaggtcta cgcccaggtc
     7081 tatgaccggg tgcttcgtga aaaacagcgg gagccggaag catgaacagg aaggcgtttc
     7141 tggcctgcgt gctgatgtgc gtcatattaa ccggctgcga aacggcgaaa aaaatcagcg
     7201 aggtgatcaa aaacccggat atccaggtcg ggagcctgaa ggagcaacct tcggaaataa
     7261 ccgtcaccct gctgactgaa ccggacacca ataccaatgc ggagggtgaa tcagccgccg
     7321 tggatgtgca actggtgtac ctgactgacg actcaaaatt gctggcagcc gactatgacc
     7381 agatagccag tacgccgcta ccggatgtgt tgggtaaaaa ctatatcgac caccaggatt
     7441 tcaacctgtt gccggacacc atcaaaaccc tgccgccggt aaagctggat gagaaaacgc
     7501 agtttattgg tgtcgtggcc tatttctccg atgaccaggc cactgaatgg aaacaaatcg
     7561 agacggtgga aggaaccggc caccattacc gcctgctggt gcatgtacgt caaagcagca
     7621 tcgaaatgaa aaaagaggat gaataattat ggcaaccacc cgcaacaagg tgatgtggca
     7681 ggaagggatg ctgatgcgcc cacaccattt ccagcaacag cagcgttaca acgactacct
     7741 ggataaccag cgtttccggg ccatgaatga tttatcctgg ggatttaccg aactcaccct
     7801 caacaatgaa ctgctggcgc agggtaagat catgattgac agcgcgtcag gcacactgcc
     7861 cgacggcacc gtcttttcta tccccgacca ggacgcactg cccgatccgc tgcacccgca
     7921 aaattttccg gacgaaagaa gccgcaatat ctacctcgct ctgccggtcg ccagtgatgt
     7981 gagaaatgaa atcagcgacg gacggcgaat cgggcgttac cggctgaatt atgccgatgt
     8041 ccgggatttg cattcagaag aaggtgacgc gcgaacattg acgctgggtc aactgacgcc
     8101 gcgcattatg agcggtgcag aagatatgag cgcctatatt acgctcccgc tttgccgtat
     8161 cagcaatcgc catgccgatg gctccctgac gttggatgac gattttatcc cctcctgcca
     8221 gaatattcag gtcagtaaga aactgcgtgt ttatctcaaa gaggtacagg gggccattgg
     8281 cggacgggca agcgatctgg caaaccgtat tggctctccg gcgcagagcg gcatcgcgga
     8341 tgtggcggaa tttatgatgt tgcagttact taaccgtaac cagacccggt ttacccatcg
     8401 cgcccgccga tcccagctcc acccggaaga tttctacctt gatcttgctg ggttgctggg
     8461 tgaactgatg acctttaccg agccgtcgcg cctgccctgc ccgcttgatg tgtatgatca
     8521 tcatgatctg accaaaacat ttaaaacact gttaccggaa gtcaaacggg cgctgcatac
     8581 cgtactgtcg ccaagagcgg tcaatctgcc gctgcatctg cgtgacggta tctggcaggc
     8641 cgatatccat gacacagaac tgctgcaatc tgccaccttt gtgctggccg tggcggcaaa
     8701 cgtgccggtc gatcagatcc agcgtcagtt tatccagcag tcgaaaattt cctcgccgga
     8761 aaaaatccgc aatatggtca gtgtgcagat ccccggtatt ccattgcgtg cccttatggt
     8821 ggctccccgc cagcttcctt accattccgg gttcagctat ttcgaactcg acaagagtgg
     8881 ccaggcctgg acagaaatgg ctgccgccgg ggccgttgca ctgcatgttt ccggcagttt
     8941 cccggatctg aacatgcaac tgtgggcgat aagagggtaa gacgatggat gaggggtcac
     9001 tgtctctgcc accatttacc ggacacgacg aaaaaagcca gcgcaactac catctcgcgc
     9061 ttcgtggcaa cagcctgaat ccgatgatcg acgcggccac gccgctactc ggtatggtaa
     9121 tgcgcctttc aaccatgaac agccagacga tgccggagca tctttttgct caggtggtga
     9181 cagatgttca ggccgtggag caactgcttc aggagcaggg ctacgaaccg ggcgtgatta
     9241 tctcgtttcg ctacatcctc tgcacgttta ttgatgaagc ggcgctcggt aacggctggt
     9301 caaacaagaa cgagtggatc aagcagtcat tgctggtcca tttccacaac gaagcctggg
     9361 gtggcgagaa agttttcatc ctgctggagc gcctgatccg tgaacccaaa cgttaccagg
     9421 atctgctgga atttctctgg atctgttttt ctctgggatt tcgcgggcgc tacaaagtgg
     9481 cagcacagga tcagggggaa tttgagcaga tctaccgccg tctgtaccac gttctgcata
     9541 aactgcgcgg cgatgcccct ttcccgctgt tacaccagga caaaaaaacc cagggcgggc
     9601 gttatcagct catcagtcgc ctgactgtta aacatatttt ttgtggtggc gttgtcgtgc
     9661 tggcgttgtt ttacctgttc tatctgttgc ggctggacag ccagacgcag gacatcctgc
     9721 accagctaaa caaattactt cgataaggac gtttatgatc cagattgatc ttcccccgct
     9781 ggtaaaacgg ctgaacctgt tctcccgcca ggcgctggag atggccgcct ctgaatgtat
     9841 gagtcagcag gcagcggaaa ttaccgtcag ccatgtgctc attcagatgc tcgccatgcc
     9901 acgcagtgac ctgcgggtta ttacccgcca gggcgatatt ggcatggaag agttgcgcca
     9961 ggcgctgacg gtagagaact acacaaccgc ccgttctgcg gacagctacc cggcgttttc
    10021 cccgatgctg gttgagtggc ttaaagaggg ctggctgctg gcgtcggctg agatgcagca
    10081 cagcgaactg cgcggcggcg tgttgctgct ggccctgctg cattcgccgc tgcgttatat
    10141 accgcctgct gccgcccggc tgttgaccgg cattaaccgt gaccgtctgc aacaggactt
    10201 tgtgcagtgg acacaggagt cggcggaatc agtcgtgccg gatgcagacg gtaaaggcgc
    10261 aggcacaatg acggacgcct ctgacaccct gcttgcccgc tatgccaaaa acatgaccgc
    10321 agacgcccgt aacggcaggc ttgacccggt actgtgccgc gaccacgaaa tcgacctgat
    10381 gatcgacatt ctctgccgcc gccgtaaaaa caacccggtg gtggtgggcg aagcgggcgt
    10441 gggcaaaagc gcactgattg aagggctggc gctgcgcatc gtggcaggcc aggtgccgga
    10501 caagctgaaa aacaccgata tcatgaccct tgacttgggc gcattgcagg ccggggcgtc
    10561 ggtgaagggt gaattcgaaa aacgtttcaa agggctgatg gcggaggtca tttcctcccc
    10621 ggtgccggtc attctgttta tcgacgaagc acataccctg attggcgcgg gcaaccagca
    10681 gggcgggctg gatatctcca acctgctcaa accggcgctg gcgcgcggcg agctgaaaac
    10741 catcgccgcc accacctgga gcgagtacaa aaaatacttc gaaaaagatg ccgccctgtc
    10801 gcgccgcttc cagttggtga aggtcagcga acccaacgct gccgaagcca ccattattct
    10861 gcgcggtctg tcggcggtct atgaacagtc tcacggcgtg ctgattgatg atgacgcctt
    10921 gcaggccgct gcgacattaa gcgagcgtta tctctccggg cgtcagttac cggacaaagc
    10981 gattgatgtg ctggataccg cctgcgcccg tgtggccatc aacctgtcgt cgccgccgaa
    11041 gcaaatctcg gcgctgacca ctctgagcca ccagcaggag gcggaaattc gccagcttga
    11101 gcgcgagctt cgcatcggac tgcgtaccga cacatcacgg atgacagagg tgctggtgca
    11161 gtatgatgaa acgctgacgg cgctggatga actggaagcg gcctggcacc agcagcagac
    11221 gctggtccgg gagattatcg cgctgcgcca gcagttactg ggcgtggcag aggacgatgc
    11281 ggcgccgttg ccggacgcag ataccgtgga ggatacgcag ccagagtcag agtcagaaca
    11341 ggataatacc ggtgccgtac cggctgatga ggccgacaga gaacaaccgg aagagaccgc
    11401 tgaaacagtt tccccggtac agcggctggc ccagctcact gccgaactgg acgccctgca
    11461 taacgaccga ttgctggtct ccccgcacgt cgataaaaaa cagattgcgg cggtgattgc
    11521 cgaatggacc ggcgtgccgc tcaaccgcct gtcgcagaat gaaatgtcgg tcatcaccga
    11581 cctgacgaaa tggctgggtg acaccatcaa aggccaggac ctggcgattg ccagcctgca
    11641 taagcatcta ctgaccgcac gcgccgacct gcgtcgtccg ggacgcccgc tcggtgcgtt
    11701 cctgctggct ggccccagcg gtgtgggtaa aaccgaaacc gtcctgcaac tggcagagct
    11761 gctctacggc ggtcgccagt acctgaccac catcaatatg tccgaattcc aggagaaaca
    11821 caccgtctcg cggctgattg gttcgcctcc gggctacgtt ggctacggtg aaggcggcgt
    11881 actgaccgaa gcgattcgtc agaaacccta ctcggtagta ctgctcgatg aagtggaaaa
    11941 agcgcacccg gatgtgctca acctgttcta ccaggcgttc gataagggcg aaatggcaga
    12001 cggtgaaggc cgcctgattg actgcaaaaa tatcgtcttc ttcctgacgt ccaacctcgg
    12061 ctaccaggta atagtcgagc atgccgatga cccggaaacc atgcaggaag tactgtatcc
    12121 ggtgctggcc gacttcttca aacctgccct gctggcgcgt atggaagtgg tgccgtatct
    12181 gccgctgtcg aaagagacgc tcgccaccat tatcgccgga aaactggccc gtctggataa
    12241 cgtgctgcgc agtcgctttg gtgcagaagt ggtcattgaa ccggaagtga cggacgaaat
    12301 catgagccgc gtcacccgcg cggaaaacgg cgcgaggatg ctggaatcgg tcatcgatgg
    12361 cgacatgcta ccgccgctct cgctgctgct gttgcagaaa atggcggcta acacggcgat
    12421 tgcccggatt cggttgtcgg cagtggacgg cgcatttacg gcagacgtgg aagatgctca
    12481 gaacgacgag tccgtcacaa aggatgaaac ggttttatga gcaggttgcg cgttttccgg
    12541 cagacaggat ggtttatcgc gggcctgatg acgggcttac ccgccactgc cgcgcccgca
    12601 gaagcatcgt caatggccgg tgtggcggtc gccgtcgcca ccacgacccc gccggatgcg
    12661 acagccacgt tacaggccat acagtcctgt cggcgggagt ccgcggcgct ggagcggctt
    12721 gactgctatg accgcctgct ggcaccgctg tccccgtcag ggtttgacgg ggcgctggtc
    12781 aaagccggtt ttgtgggcga ggcctggacc cgcgccacag agcaggaaaa acgccgcgag
    12841 ggcaacacca cagaactgct ggtgacgcag gtgccggggg agcgcccgac ggtggtgata
    12901 accaccccgg ctatcggtca tgttccgcca cgcccggtgc tgatgttcag ttgcgtggac
    12961 aatatcaccc ggatgcaggt ggcgctgatg caccctctgg atgtccatga tatcgccgtc
    13021 actctgaatg ccgacaaccg ggcattgcgc agccactggt ttgtgcgcga gaacggcacc
    13081 ctgctggagt ccagccgggg gttatccggg attgatgaaa ttaagcaact gttcggcgca
    13141 aaaacgctga cggtggatac cggcgcagac aatgccgccg gaaagctgac ctttaacatt
    13201 gacgggctgg caagggcgat tgctcccctg cgcgatgcct gccactgggc gggagaataa
    13261 aaatatggca atggacttac gcgatccgaa tgtctggata tcgcacctgc tggaaaacct
    13321 gccggaagag aaactggcat cggcgctgaa agatgacaac ccgaactggg agtatatcga
    13381 cggcgaaatc gtcaagctgg gatcgctggc ccacagtcaa ctggatatcc cggaactcca
    13441 gcgccggggg ctggtcattc tggcttcgga gagtaaggat tttcgtctgc tggcgcatct
    13501 gctgagaacc ctgcaacacg ccggtgatcc tcatctcgca ttgcgcctgc tggcgctgta
    13561 tgtggagcat tactgggcgg tggctgcgcc gcagaatatg gcgcataaaa aacgctttgc
    13621 cagccaggtc attaaacgct ttgaaacagg tattgaaggt ttttcacaga acgccgccac
    13681 agagcagcgc gatgcgctgt caggggaact ggcaaaactg gcgcaatgct ggcagtcaca
    13741 taacgccccg gaactggcac aggctactga tgatcttttt gccctgtacc agcgtgcgtt
    13801 taatcgtgcg gcaccggctc ctgtccccac tccggcggcc tccggtagtt caccacagac
    13861 caccgccacg tctgaaagcg gcgtgacgca acccagtgct ccggctcccc aaatcgtcat
    13921 cgacagtcac gacgacaaag cctggcgcga cacgctgtta aaagtggcgg ccattttatg
    13981 tgaacgccag ccggattcac cgcagggcta ccgtctgcgc cgccatgcgc tttggcagaa
    14041 catcaccagt acgccccagg cggaaagcga tggccgtact ccactggctg cggtctccgc
    14101 cgatatggtg gcagattacc atgctcagct cggtagtgcc gatatggcgc tgtggcagca
    14161 ggtggagaaa agcgtactgc tggctcctta ctggctggat ggtcactgcc tttctgcaca
    14221 gacggcgctg cgtctgggtt acaaacaggt ggcagacgcc atccgcgatg aggtcatccg
    14281 cttccttgag cgtctgcccc agcttaccgg gctgctgttt aatgaccaca caccgtttat
    14341 cagtgagcag acgaaacaat ggctggcagc ttcgcccgac gccaaagttg caccggttgc
    14401 gcaaatcggt gaggaatcga aggcagccag agcctgtttt gctgagcagg gtctggaggc
    14461 ggcgctgcga tatctggaca tgctacccga aggcgatccc cgcgatcagt ttcaccgcca
    14521 gtaccttgcc gcacagttga cggaagaggc ggggctggta cagcttgcgc agcagcagta
    14581 ccggatgttg ttcaggatgg gattgcagat gatggtggca gactgggagc cgtccctgct
    14641 tgagcagcta gaacaaaaat tcacggcaga acaataaaag agaacaggag tttttgtgtt
    14701 cagattacca acaccccgac tactcagcgg actcaaatca gccctgcgac cggcgatgcc
    14761 ccggtttaaa gtctctgctt tctggctgct gatactggcg tggatttttc tgctggtgtg
    14821 gatctggtgg aaaggcccga cgtggacgct gtatgaagaa cagtggctca aaccactggc
    14881 gaaccgctgg ctggcaacgg cggcgtgggg gattattgcc ctgatgtggc ttaccgtccg
    14941 ggtgatgaag cgcctgcaac agcttgaaaa aatgcaaaag caacagcgcg aggaagccgt
    15001 tgatccgctc agcgtggaac tgaacgccca gcagcgttat cttgaccgct ggctgctgcg
    15061 cctgcaacgc tatctcgaca accgccgttt cctgtggcag ttgccgtggt atatggtcat
    15121 cggcccggcg ggcagtggca aaacgacact gctgcgcgag gggtttccgt ccgacattat
    15181 ttatgccccg gagggcgcgc gcggcgcaga acaacgcctg taccttacgc cccatgtcgg
    15241 taaacaggcg gtgatctttg atatcgacgg cacactctgc gctcccgctg atgcggatat
    15301 cctgcatcgc cgtctgtggg aacatgccct cggctggctg aaagaaaagc gcgcgcgcca
    15361 gccgctgaac gggattattc tgacactcga tttacccgat ctgcttaccg cagacaaacg
    15421 ccgccgcgag catctgttac agacgctgcg cagccgcttg caggatatac gccagcatct
    15481 tcactgccag ttaccggttt acgtggtact cacccggctt gatttattgc agggttttgc
    15541 cgccctgttc cagtcactga acagacagga tcgcgatgcg attctgggcg tcacgttcac
    15601 ccgccgcgcc catgaaaatg atgactggcg aacagagttg aatgctttct ggcagacatg
    15661 ggtggatcga atgaatctgg cgttgccgga tctgatggtc gctcagactc acacccgcac
    15721 gtctttattc agtttttccc gccagatgca gggaagccgt gaaccgctgg tgtcactgct
    15781 tgagggtctg cttgatggcg aaaatatgaa cgtgatgctg cgtggtgtct atctcacctc
    15841 ttcgcttcag cgtgggcaga tggatgatat attcacccag tctgccgccc gccagtaccg
    15901 gctgggcaat aacccactgg cgtcctggcc cctggtggac accgcgcctt atttcacccg
    15961 cagcctgttc ccgcaggcat tactcgcaga gcctaatctg gcaacagaga gccgcgcctg
    16021 gctgatacgt tcccgtcgcc gcctgacagt tttctctgcc acaggcggcg tggcagcact
    16081 tctgctcatc accggctggc atcactatta caacggtaac tatcagtccg gcatcaccgt
    16141 gcttaagcag gccaaagcct ttatggacgt gccgcctccg cagggggaag atgactttgg
    16201 caacctgcaa ctgccgcttc tgaacccggt acgcgatgcc acactggcct atggcgactg
    16261 gggcgaccgc agccgtctgg ccgatatggg actgtaccag ggacgacgta tcgggcctta
    16321 tgtggaacag acctatctgc aactgctgga gcaacgttac ctgccctcgc tgtttaacgg
    16381 gctggtcaaa gcgatgaacg ccgcgccgcc ggagagtgaa gaaaaactcg cggtgctgcg
    16441 cgtaatgcga atgctggagg acaaaagcgg acgtaacaat gaggtggtga agcagtatat
    16501 ggcaaaacgc tggagcgaaa aattccacgg ccagcgcgat atccaggcac aactgatgtc
    16561 ccatcttgac tacgcgctgg ctcatactga ctggcatgca gagcgtcagg cgggcgacgg
    16621 tgacgccatc agccgctgga cgccatatga caagcccgtg gtatcagcac agaaagaact
    16681 gagcaaactg cctgtctacc agcgggttta ccagagcctg aaaacgcggg cgctgggcgt
    16741 tcttcctgcc gacctcaatc tgcgtgacca ggtagggcca acctttgacc aggtgtttac
    16801 atctgccgat gacaacaaac tggttgttcc acagtttctt acccgttacg gcctgcaaag
    16861 ctattttgta aaacagcgcg atgaactggt tgaactgacg gcgatggatt cctgggtact
    16921 taaccttacc cgcagcgtga aatacagtga cgccgaccgc gcggaaatcc agcgccagtt
    16981 gaccgagcag tatatcagcg actacaccgc cacctggcgg gccgggatgg acaatctgaa
    17041 tatccgcaat tttgagtcca tcggacaact gaccggggcg ctggagcagg ttatcagcgg
    17101 cgaccagcct ttgcagcggg cgctgaccgt cctgcgtgac aacacacagc caggcgtctt
    17161 ttctgaaaaa ctctctgcca aagaacggga ggaagccctg gcagagccgg attaccagtt
    17221 actcacccgc ctcgggcatg aattcgcccc ggaaaacagt accctggcag tacagaaaga
    17281 caaagaaagc acgatgcagg ccgtgtatca gcaactcacc gagttgcacc gctacctgct
    17341 ggcaatccag aacgcgcctg taccagggaa atcggcgctg aaagccgtgc agttacggct
    17401 tgatcagaac agcagcgatc cgatattcgc cacccgccag atggcaaaaa cgctgcctgc
    17461 tccgctcaac cgctgggttg gcagactgac tgaccaggcc tggcatgtgg tgatggtgga
    17521 ggctgttcat tatatggaag tggactggcg cgacagcgtg gtgaaaccgt ttaacgagca
    17581 actggcaaat aactatccgt ttaatccgcg ttctgcacag gatgcctcac tggatgcctt
    17641 cgaacgcttc tttaaaccgg atggcatact ggataccttc taccagcaga acctgaagct
    17701 gtttatcgat aatgacctga gtctggagga tggcgataac aacgtcatta ttcgcgaaga
    17761 tattattgcg caactggaaa ctgcgcagaa aatccgtgac atcttcttca gcaaacagaa
    17821 cggtctggga acatcctttg ccgtggaaac ggtatcgctt tcaggcaata aacgccgcag
    17881 tgtactgaac cttgacggtc agttagtcga ttacagccag ggccgtaact ataccgccca
    17941 tctggtctgg cctaacaaca tgcgcgaagg caacgaaagt aagctgacgc tcatcggcac
    18001 cagcggcaac gcgccgcgca gtatcagctt cagcgggccg tgggcgcagt tccgcctgtt
    18061 cggggccgga caactgaccg gagtacagga tggcaacttt accgtgcgct ttagcgtgga
    18121 cggtggcgcg atgacctacc gtgtgcatac cgacacggaa gataacccgt tcagcggtgg
    18181 gttgttcagc cagtttggtc tgtcagacac actgtactga ggaggcaggg atgaatagta
    18241 acgtactgac acaaactatc gttaccggca gtgacccgcg cgggctgccg gaattcagcg
    18301 ccatccgcga ggaaataaac aaagccagcc acccgtcaca gcctgagctg aactggaaac
    18361 tggtggagtc gctggcgctg gcgattttta aagccaacgg tgtggattta cacaccgcca
    18421 cctactatac gcttgcccgg acacggacac agggactggc gggattctgc gaaggtgcgg
    18481 aactgctggc ggcaatggta agccacgact gggataagtt ctggccgcag ggcggcccgg
    18541 cgcgtactga aatgctggac tggtttaact cccgaaccgg caatattctg cgtcagcaaa
    18601 tctcctttgc ggaatccgac ctaccactga tctaccgcac agagcgggca ttgcagctta
    18661 tctgcgataa gctccagcag gtggaactga agcgcgttcc gcgcgtggag aatctgctct
    18721 attttatgca gaacacgcgt aaacggcttg aaccccagct gaagagtaac actgagaacg
    18781 ccgcacagac cacggtcaga acgctgattt atgccccgga aacacaggca tcttccacac
    18841 cagaagcggt agtgcctccc ctgcccggcc tgcctgagat gaaagtggaa gtgcgcagtc
    18901 tgacagagaa tcccccacag gccagtgtga taaagcaagg cagtacggta agagggttta
    18961 tcgcagggat cgcctgttca gtggctgtcg cctcagcatt gtggtggtgg caggtctatc
    19021 cggtgcagca gcaactgtta caggttaacg acaccgctca gggcgcagca acggtgtgga
    19081 tggcctcacc tgaactcgaa aactatgagc gcaggctgca acaacttctt gatacctccc
    19141 cggtacagcc gctggaaacc gggatgcaga tgatgcgtgt tgccgacagt cgctggccgg
    19201 aaagcctgca acagcaacag gcctcgacac aatggaatga ggcactcaaa acccgcgcac
    19261 agagtagccc gcagttgcgt ggctggttgc agacccgcca ggacttacat gcttttgcag
    19321 atctagtgat gcagcgcgag aaagagggac taaccctttc ctatatcaaa aatgtcatct
    19381 ggcaggcgga gcggggactg gggcaggaaa cacccgttga gtctctgttg acgcagtacc
    19441 atgatgcccg tgcgcagaag cagaatacag atgcgctgga aaaacaaatt aatgagcgac
    19501 tcgaaggcgt gttaagccgc tggctgctgc tgaagaataa cgtcatgcca gaggcggcaa
    19561 ccggtaccac ggccgaaaaa taattattta attaaaggag attatcatgg caaatataag
    19621 ttatttatct ttgagtggag agacacaagg gctaatttca gcaggttgct caacgcttga
    19681 ttctgtcgga aacaaagcgc agccagaaca taaagatcag atcatggttt atgcgttaat
    19741 gcattccatc tcaagaagtc aaaatgtcaa tcatcatgaa ttaattatca ccaaaccggt
    19801 tgataaatcc tcaccattgc tggcaaaagc acttagtgac aatgaaaaaa tggcaatatg
    19861 tgaatttata ctctaccgaa ccagtaaagc gggtatttac cagccttatt ataaaataaa
    19921 tctgtcaaaa gcccgaatat caagtattga ttttgtcaca ccgcatgctg tcctggaaaa
    19981 agaactggaa ccacaggaga gaattgcctt tatttatgaa gatatttcct gggagcatac
    20041 gttagccggt acgaatgcaa tgagtaaatg gcaggatcgt gtacagtaac ttactgtaat
    20101 aacataataa attactattg cggagagata aaatggctgg tttaaggaat aacaacaaca
    20161 cacagaatgc acagtgggct gattatgtgg gtgacattct tcggggggct cagccgataa
    20221 accagctagt acctcagcac ccttatctta acgacgtccc actcattgat gagttaaggc
    20281 atcagaacac tcatcatgtt attcccttga cacttgatgt tgcaaaaaaa aatactcagc
    20341 ccaataacat catttgatta cattcatttt atcactacac atccttcagg tataaaagat
    20401 acactcgcct ggttagttaa cgcgggtaaa ttaatgactg agtttgatga caatgggaaa
    20461 attatattta accttaatgc cttaaagtat acaaaagcaa gttactttga aatacttgga
    20521 gagaaatata taaaaatcac aacatccagc ccctggcttt tagaaaagct cggtaaatat
    20581 attttctctt cacgagcgcc tcaagttctg gaactcgcta ttggctggcg aggagcttta
    20641 tctgaatcaa taaaaggagt taagttctgt atctggtttt cagtagcctg gagaaccata
    20701 gaatttatta tgagttcaga gcgagatctg gttaattttc ttggcgattt ttcaatggat
    20761 gtggccaaag cagttatagc cggtggtgtt gcaaccgcta ttggaagtct ggcttctttt
    20821 gcctgtgtta gctttggctt tccagtaatt cttgtcggag gagcaatttt actgacaggg
    20881 atagtgtgta cagttgtttt aaatgaaatc gatgctcaat gccatttatc agaaaaatta
    20941 aaatatgcaa ttagagatgg actaaaacgg caacaggaac ttgataaatg gaaaagggaa
    21001 aacatgactc catttatgta tgttcttaac actccacccg tgatatgagg attaaccatg
    21061 cgtttacatg ttaaattaaa agagtttctt tcgatgtttt ttatggccat attatttttc
    21121 cctgcattca atgcttcttt gttttttacc ggagttaaac ctctatattc aataataaaa
    21181 tgctccacgg aaatatttta tgattggaga atgttgattc tgtgcttcgg tttcatgtcg
    21241 ttttcctttt taaatattca tgtaatttta cttacgataa taaaatcatt cttaatcaaa
    21301 aaaacgaagg tagtcaattt cgccacagac ataacaatac agctaactct tatattttta
    21361 ctaatagcta tcgtaatagc acctttaata gctccttttg tcacaggata tgtcaatact
    21421 aattatcacc cctgcggtaa taatacggga attttcccag gagcaatcta tattaaaaat
    21481 ggcatgaaat gtaataatgg atatatttct cgcaaggaag attcagcagt taaataatta
    21541 aatattctaa aacaaacagt ttttaactcc ggattccatg tcctttcttg cactgtcttt
    21601 gtcaagaaaa atttcttatg caaagaattt tgtattattg ccgccagaaa ttcctggcgg
    21661 cagatataat ttattattga tagcggttaa ccaaatcttt taccatcgct ttataggtct
    21721 tttgcttaag ttgcatgatt tgctgctctg tcatatcagt cgtactggct tcattcttgt
    21781 cgggcaatgg tggaagcact ttatattcat aattcaccgg cgaccaggta gcccactcac
    21841 ctgttgccac atccaccagc gtgaaacgta ccaggtagcg cagggagata gcgtcagtga
    21901 gtttgtcatt tttatagtcg gaccatactg ttgatttcag cgcagagtca tattttcctg
    21961 tctgcaacat atcctggtaa acaataatcg ctttctgatg tccttttgca gccacaaaac
    22021 gcagtgcctg catccagttc atattctcag cactggcaac atcctcgttc tcatttttat
    22081 ctttgttttt gttacaagtc agaggctttt gcctgtccgg gataccagag aatgtggaaa
    22141 cagtataata ttttcgcatc tcctcctgca taatggtttc cggtgcgcgg ttgccggatt
    22201 gaacaagtat tacaggagaa ttaaggggaa cgcggaactc atctccatca agcgcagctt
    22261 gaatatcctc ttcggatacg gccagagtgg tttcattacc gaataaatct ttgtctgtca
    22321 gttgctgggg agtgttttta ttaatattac tttgtgtgac ttgagctttt ttcttctttt
    22381 catcaataga agattcagag gcacatcctg caagcagcga gaataacaca catatgcaac
    22441 aaagcttttt tgaattcata attggacact ccctcgcctg tcataatgat tagaatattt
    22501 agcattgata acggactcta attatatacc tcacttaaat aataacaaac aaaatcaatt
    22561 tgaaatatac attcatttaa tcaatataaa tttattctct agaaatatat ttaagctgaa
    22621 tggttcgata tcattattga attttaatga atgagacaac aagcgagaat cacgcaccta
    22681 cgccagagta aaatcattga acagagaaat ggtggaattc aaatacaaaa aaaccgcaaa
    22741 attaaaaatc ttgcggctct ctgaactcat tttcatgagt gaatagtggc ggaacggacg
    22801 ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc
    22861 tccgcgttgt gttccgttgg gaacgaggcg aatattacgg attgcctcga ccttcgtcaa
    22921 cggtttttct catcttttga atcgtttgct gcaaaaatcg cccaagtcgc tatttttagc
    22981 gcctttcaca ggtatttatg ctcgccagag gcaacttccg cctttcttct gcaccagatc
    23041 gagacgggct tcatgagctg caatctcttc atctgtcgca aaaacaacgc gtaacttact
    23101 tgcctgacgt acaatgcgct gaattgttgc ttcaccttgt tgctgttgtg tctctccttc
    23161 catcgcaaaa gccatcgacg tttgaccacc ggtcatcgcc agataaactt ccgcaaggat
    23221 ctgggcatcg agtaatgccc cgtgcagcgt tcgtttactg ttatctattt cgtagcgagc
    23281 acataacgca tcgaggctgt tgcgcttacc gggaaacatt ttcctcgcca ccgcaaggct
    23341 atcggtgacc ttacagaaag tattggtctt cggaatatcg cgcttaagca acgaaaactc
    23401 gtagtccata aagccgatat cgaacgctgc gttatggatc accaactccg caccgcgaat
    23461 atagtccatg aactcatcgg ctacttcggc aaacgtgggc ttatcgagca aaaattcatc
    23521 ggcaatacca tgtacgccaa aggcttccgg atccaccagc cgatcgggtt tgagataaac
    23581 atggaagtta ttgcccgtca ggcgacggtt caccacttca acggcaccaa tctcaatgat
    23641 cttgtggcct tcatagtgcg caccaatctg gttcataccg gtggtttcgg tatcgagaac
    23701 gatctggcgt gtaattgcag tgctcatagc ggtcatttat gtcagacttg tcgttttaca
    23761 gttcgattca attacaggaa gtctaccaga gatgcttaaa caggtagaaa ttttcaccga
    23821 tggttcgtgt ctgggcaatc caggacctgg gggttacggc gctattttac gctatcgcgg
    23881 acgcgagaaa acctttagcg ctggctacac ccgcaccacc aacaaccgta tggagttgat
    23941 ggccgctatt gtcgcgctgg aggcgttaaa agaacattgc gaagtcattt tgagtaccga
    24001 cagccagtat gtccgccagg gtatcaccca gtggatccat aactggaaaa aacgtggctg
    24061 gaaaaccgca gacaaaaaac cagtaaaaaa tgtcgatctc tggcaacgtc ttgatgctgc
    24121 attggggcag catcaaatca aatgggaatg ggttaaaggc catgccggac acccggaaaa
    24181 cgaacgctgt gatgaactgg ctcgtgccgc ggcgatgaat cccacactgg aagatacagg
    24241 ctaccaagtt gaagtttaag cctgtggttt acgacattgc cgggtggctc caaccgcctg
    24301 gcgaattcgt ggcttgtttt tactctgttt catcggattt agcgttaaag gaatagtccg
    24361 tttccgggca acaataagtt gtaagcaacc aagcgcagga atatgcgcat tcaatagttt
    24421 tcctccgtgt ttgttccacg ggagaacgtg gaaacggctg gcgtgtagca cttcaaaatt
    24481 caacaaagag agccagtcca gctgccgcat cagagtaaac atccggctgt tatagggcga
    24541 ggttttgcgc aataccggca caagtttgcg taatcccata aaactgatgg gattgaagcc
    24601 actaatgacc agccagccat catcaatcaa tacccgatcg gcttcacgca ataaacgatg
    24661 cggatcggtg caccacggca atgtatgtgc cagtagacaa acatcaacgg atttatcggc
    24721 aaaaggaaga tgaagtgggt ccgcctgtac ctggacgggc attccttgcg cagaaacatt
    24781 cacttgatga gaaaccgcgc acgcttcgca attgatttct gcgcttaaat tgccaatctt
    24841 aagcagatga aaaccataca ttttagtgaa ccacgggttg agctggcgct ccagcgcctt
    24901 gcgataaagc tttccccagg gcaaatcgcc ccagcaatca ggagccacga cagtttgagg
    24961 gactcttgcc ggtttcatca caaccttccg tttcacactg agaggtaatc tatgaatctt
    25021 aacagtattc ccgcctttga tgacaattac atctgggttt tgaatgatga agcaggtcgc
    25081 tgcctgattg tcgatcccgg agacgcagag ccagtattaa acgccattgc cgccaataac
    25141 tggcaaccgg aggccatatt tctcacccac catcaccacg atcacgttgg cggcgtaaaa
    25201 gaactggtgg aaaagtttcc acaaattgtg gtgtatggtc cacaagagac acaagataag
    25261 ggaacaacac aggtagtcaa agatggcgaa actgccttcg ttttggggca tgaatttagt
    25321 gtaattgcta cgccgggtca cactttagga catatctgtt acttcagtaa accttatcta
    25381 ttttgcggcg acacactgtt ttctggtggg tgtggtcggt tgtttgaagg gacagcatca
    25441 caaatgtatc aatcacttaa aaagttaagt gcgttacctg acgatacatt ggtatgttgt
    25501 gctcatgaat ataccttatc aaatatgaag tttgctttga gtattcttcc gcacgatttg
    25561 tccataaatg attattatcg taaagttaag gagttacggg caaaaaatca aataacacta
    25621 cccgtaattc tgaaaaatga gcggcaaatt aatgtttttt taagaacgga agatattgat
    25681 ttaattaatg taattaatga agaaacatta ttgcaacaac ctgaagagcg ttttgcatgg
    25741 ttaaggtcaa agaaagatag gttctgataa aactttcttg tcatcggctc cgttcgccgt
    25801 tatgatcggt cgtcttttaa gcaactattg acacacacat gaaggcaaaa gcgatattac
    25861 tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa cgttcaacag cacgcacaga
    25921 gcctttctgc agctggtcaa ggggaagcag caaagtttac aagtcaggca cgatggatgg
    25981 acgatgggac gtctatcgcg ccagatggtg acttgtgggc tttcattggc gacgagctaa
    26041 agatgggaat tccggaaaat gaccggattc gcgaacagaa acagaaatat ttacgcaata
    26101 agagctatct ccacgatgta actttacggg cagagccgta tatgtactgg atagcagggc
    26161 aagttaaaaa acgtaacatg cctatggaac tggtactact acccatagtg gagagcgctt
    26221 ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat ctggcagatc attccgagca
    26281 cggggcgcaa ttatggtttg aaacagaccc gcaattatga cgcgcgtcgc gatgttgttg
    26341 cttcaacaac tgccgcgctg aatatgatgc agcgtctgaa caaaatgttt gacggcgact
    26401 ggcttctgac cgtagcggct tataacagcg gcgaaggtcg ggtcatgaag gcaattaaaa
    26461 cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt accgttgccg caggaaacga
    26521 agcagtacgt gcctaaaatg ctggcattga gtgatattct caaaaacagc aagcgttatg
    26581 gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc gcgtgtgcac ctgagcagcc
    26641 cggttgaaat ggcgaaggta gcagatatgg cggggatttc cgtcagcaag ctgaagacat
    26701 tcaacgctgg cgtaaaaggc tccacgctgg gcgcaagtgg cccgcagtac gtgatggtgc
    26761 caaagaagca tgcagatcaa ctgcgcgaat ctctggcttc aggcgaaatt gctgctgtac
    26821 agtcgacgct ggttgccgac aatacgccgc ttaacagccg tgtttacacc gtacgctctg
    26881 gcgacacgct ttcaagtatc gcttcacgtc tcggcgtaag caccaaagat ttgcagcagt
    26941 ggaacaaact gcgcggatct aagctgaagc caggccaaag tttgacgatt ggtgcaggca
    27001 gtagcgcaca gcgactggca aacaacagcg atagcattac gtatcgtgtg cgcaaaggcg
    27061 attcgctttc aagcattgct aaacgccacg gcgtgaacat caaagatgtg atgcgctgga
    27121 acagcgacac tgcgaatctg caaccaggcg ataagctgac gttgtttgtg aaaaacaaca
    27181 acatgccaga ttcctgacaa accagataat aaaaaggcac cgattccccc ggtgcctttt
    27241 ttatttatgc cgctttatgt gcatctacca tgatgatatc actggtgaaa aagccatcat
    27301 tctgcaaggc aaaatacgtt ctcacctctg tcgatgcgct ctgttggtaa atgcgaatag
    27361 cgtctactaa cgcttctggc gtacgcattc tcgcgaccca tgaagaaaat tccagcggta
    27421 acttatctgt aattaaatta tcaactatca gattggcttc attgattaac gtcaaccact
    27481 caccgctggc gtagtttcgt acgtgagagg tatcgcgtaa tgcttctacc gtctgtaacc
    27541 agatgtcgcg cactgggtga cccggagaca ttacgtccat cacaatcagc ctaccgccag
    27601 gtttcaatat cctattcact tctcgcagtg ctgcaccaac atcatgccag tgatgggcag
    27661 aataacggct gataacaata tcaaatgcgt tatcggcaaa tggcagactt tcggcatatc
    27721 cctggcgggt ggtgatattt ttcagttgcc gggcttcggc agcttgtgcc acgacatcca
    27781 gcatgtgggc agataagtca tacgccacca ccgcgctcac gttttgcgca gcgccaaacg
    27841 ttggtctgcc ctgtggcaga cctgacatac cttatttatc aggcttgccg ggactgaatt
    27901 caacgagtag cggattgtga tcggaagcgc gcgtaaccag tacagaagct tcactgacgt
    27961 tcagaccacg gtagaaaaca aaatcgagcg ggcgaccaaa cgcccgacgg cgctgatcat
    28021 cggtaaaacg cacctggcgc agcgacattt cccgcgcaaa gcgatataac gcgttcatcc
    28081 ttctacggct ccaggcattg aaatctcccg ccataatgac cgggccgctg tggtgagcta
    28141 tctgatcgcc aataggaagt aactgcttac tatagacatc cacgcccaga ctgaagttga
    28201 cggcgtgtat attaaccacc atcaacaggc gggtgtcagg taatggatag accgtcacca
    28261 gtgccgactt cgccagacgc aaaatgggtt ctcgttcacg taacgggcag caatacactg
    28321 gatgtgccgc cgaaagggtc attacgccag aaggatgttg tggcagcacg aaagcgggta
    28381 cctgatcggc ggcaagatag ttagcggtcg caaactgtac taactctggc gttgtctgcg
    28441 cttcctgcaa taacaccaga tgtgcatctt tgccgtagtt ctttaatacc gacaaccatt
    28501 cagcgcgttg ctgtttgtat atgttccaca ccaggatccg aatacgctct tcggtactta
    28561 acggttcccc aggtggtaat gcctggccga tgctcgcaaa agaccccggc ggtaagatcc
    28621 tttccgcagg ttgtccggca acatagcgca tggcataggt gttttttcgc acttttgact
    28681 ttgaaacctc tattacgtca acccttccgc actcgggagg gttctactgt tatagggatt
    28741 ttagctcact ctttcaacga gcaattgtat attgttatgt aagcaagtgc ttacaccggt
    28801 cagaattttg ctcattatgg agaacattaa atcgcatgac atatcacgca tcctgatggt
    28861 ttaatgacaa ataaattcaa aatatctctt ctattttgca acaggagcaa aaatgaaagc
    28921 cacgtcggaa gaactcgcca tttttgtttc ggtcgtcgaa agcggcagct ttagccgggc
    28981 agcggaacaa ttagggcaag caaactcagc ggtaagccgg gcggtgaaaa agctggagat
    29041 gaaacttggc gttagcctgc ttaatcggac cacgcgacaa cttagcctga cggaagaagg
    29101 cgagcgttat tttcgtcgcg tacagtcaat tttgcaggag atggcagcgg cagaatcaga
    29161 aattatggag acgcgtaata caccgcgtgg actgttacgg atcgatgccg caactccagt
    29221 ggtgctgcac tttctgatgc cgttaattaa gcctttccgt gaacgctatc cggaagtcac
    29281 tttgtcgcta gtctcctccg aaacgattat taatttgatc gaaagaaaag tggatgtcgc
    29341 gatacgcgct ggtacgttaa cggattccag cttacgtgcc aggccgttat ttaacagtta
    29401 tcgaaaaatt atcgcctccc ccgattatat ttcccgctac gggaagccag aaacgatcga
    29461 cgatttaaag caacatattt gcctgggatt cactgaaccc gcttccctca atacctggcc
    29521 gatagcccgt agcgatggac aattacatga ggtgaagtac ggtttgtcat ccaatagtgg
    29581 ggaaacactg aaacagcttt gcctgagtgg caacgggatt gcgtgtttgt ccgactacat
    29641 gatcgacaaa gaaatcgctc gcggagaatt ggtggagtta atggcagata aagtgttgcc
    29701 agtggaaatg ccattcagtg ccgtctatta cagcgaccgt gcggtaagta cgcgcatccg
    29761 ggcttttatc gatttcctta gcgagcatgt aaaaacagct cccggaggag ctgtcagaga
    29821 ggcttaatcc cattcaggag ccagaccttc cgggctaacc aggcggtcgt tgcaatccag
    29881 tgcggcgatc gcttttttat cttcggcatc aagctgtaaa ttttgtgcct taagattact
    29941 ttccaggttt ttacgtttag tagaagaagg aattactgag taaccttccc ccatagccca
    30001 cgccagaatc acttgtgccg gagtcgcatt gtgtttagct gcgatacgag caataacctc
    30061 atctttcagg gccttaccat acgccagcgt catataggaa gtaatatgga tgccgtgctg
    30121 tttagcccag gcaaccactt tacggttttg cagataagga gagagttcaa tctggttagt
    30181 agcgatgttt tcagcaccaa cagcagcaat cgctttttcc atcaacggga tcgtgaagtt
    30241 ggaaataccg atctcacgcg tcagcccttg ttttttggct tccagcagcg cctgcataaa
    30301 ctcttcaaca gagacttcat cgtttggtga cggccagtgg attagcgtca gatcaacata
    30361 atcggtacgc aatttttgca ggctctcttt cagacttggg atcaatttgt ctttgctgag
    30421 attttcaatc cagattttag tggtgatgta gagttcatga cgtggcacgc cactttctgc
    30481 aatcgcctga cctactgcgg cttcgttatc atagatttgt gcggtatcaa ttgcgcgata
    30541 accaagttca agcgccgtta tcacagatga aataacaacg tcgtctttca gacggaaagt
    30601 acctaaacca aatgcaggga tagccataat atgcctcttt taatgctatt ttcgttaact
    30661 gaggtgatta tgggcttcag actgttgagg aaaaagagcg taaaatgcag aggatttttg
    30721 cgattctggc aataatagat atacagacga aaaaaaacct cgagcgttaa ctcgaggctt
    30781 cttaata
//
LOCUS       DBBGLQ010000049        30107 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.049, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000049 DBBGLQ010000000
VERSION     DBBGLQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.049"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(205..1869)
                     /gene="glnS"
                     /locus_tag="ACIYSB_003012"
     CDS             complement(205..1869)
                     /gene="glnS"
                     /locus_tag="ACIYSB_003012"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGC5430016.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2072..4018)
                     /gene="nagE"
                     /locus_tag="ACIYSB_003013"
     CDS             complement(2072..4018)
                     /gene="nagE"
                     /locus_tag="ACIYSB_003013"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGC5430017.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4351..5151
                     /gene="nagB"
                     /locus_tag="ACIYSB_003014"
     CDS             4351..5151
                     /gene="nagB"
                     /locus_tag="ACIYSB_003014"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGC5430018.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5211..6359
                     /gene="nagA"
                     /locus_tag="ACIYSB_003015"
     CDS             5211..6359
                     /gene="nagA"
                     /locus_tag="ACIYSB_003015"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGC5430019.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6368..7588
                     /gene="nagC"
                     /locus_tag="ACIYSB_003016"
                     /gene_synonym="nagR"
     CDS             6368..7588
                     /gene="nagC"
                     /locus_tag="ACIYSB_003016"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGC5430020.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNTIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7636..8388
                     /gene="nagD"
                     /locus_tag="ACIYSB_003017"
     CDS             7636..8388
                     /gene="nagD"
                     /locus_tag="ACIYSB_003017"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGC5430021.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8439..8537)
                     /locus_tag="ACIYSB_003018"
                     /pseudo
     CDS             complement(8439..8537)
                     /locus_tag="ACIYSB_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8785..10449
                     /gene="asnB"
                     /locus_tag="ACIYSB_003019"
     CDS             8785..10449
                     /gene="asnB"
                     /locus_tag="ACIYSB_003019"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGC5430022.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10829..10905
                     /locus_tag="ACIYSB_003020"
     tRNA            10829..10905
                     /locus_tag="ACIYSB_003020"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10863..10865,aa:Met,seq:cat)
     gene            10915..10999
                     /locus_tag="ACIYSB_003021"
     tRNA            10915..10999
                     /locus_tag="ACIYSB_003021"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10949..10951,aa:Leu,seq:tag)
     gene            11023..11097
                     /locus_tag="ACIYSB_003022"
     tRNA            11023..11097
                     /locus_tag="ACIYSB_003022"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11055..11057,aa:Gln,seq:ttg)
     gene            11132..11206
                     /locus_tag="ACIYSB_003023"
     tRNA            11132..11206
                     /locus_tag="ACIYSB_003023"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11164..11166,aa:Gln,seq:ttg)
     gene            11222..11298
                     /locus_tag="ACIYSB_003024"
     tRNA            11222..11298
                     /locus_tag="ACIYSB_003024"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11256..11258,aa:Met,seq:cat)
     gene            11346..11420
                     /locus_tag="ACIYSB_003025"
     tRNA            11346..11420
                     /locus_tag="ACIYSB_003025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11378..11380,aa:Gln,seq:ctg)
     gene            11458..11532
                     /locus_tag="ACIYSB_003026"
     tRNA            11458..11532
                     /locus_tag="ACIYSB_003026"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11490..11492,aa:Gln,seq:ctg)
     gene            complement(11686..12861)
                     /gene="ubiF"
                     /locus_tag="ACIYSB_003027"
     CDS             complement(11686..12861)
                     /gene="ubiF"
                     /locus_tag="ACIYSB_003027"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGC5430023.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            13007..14431
                     /gene="miaB"
                     /locus_tag="ACIYSB_003028"
     CDS             13007..14431
                     /gene="miaB"
                     /locus_tag="ACIYSB_003028"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGC5430024.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14584..15624
                     /gene="ybeZ"
                     /locus_tag="ACIYSB_003029"
     CDS             14584..15624
                     /gene="ybeZ"
                     /locus_tag="ACIYSB_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGC5430025.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15621..16088
                     /gene="ybeY"
                     /locus_tag="ACIYSB_003030"
     CDS             15621..16088
                     /gene="ybeY"
                     /locus_tag="ACIYSB_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGC5430026.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16178..17056
                     /gene="corC"
                     /locus_tag="ACIYSB_003031"
     CDS             16178..17056
                     /gene="corC"
                     /locus_tag="ACIYSB_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGC5430027.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17081..18619
                     /gene="lnt"
                     /locus_tag="ACIYSB_003032"
     CDS             17081..18619
                     /gene="lnt"
                     /locus_tag="ACIYSB_003032"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGC5430028.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            19016..19924
                     /gene="gltI"
                     /locus_tag="ACIYSB_003033"
     CDS             19016..19924
                     /gene="gltI"
                     /locus_tag="ACIYSB_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGC5430029.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFANLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            20094..20834
                     /gene="gltJ"
                     /locus_tag="ACIYSB_003034"
     CDS             20094..20834
                     /gene="gltJ"
                     /locus_tag="ACIYSB_003034"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGC5430030.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20834..21508
                     /gene="gltK"
                     /locus_tag="ACIYSB_003035"
     CDS             20834..21508
                     /gene="gltK"
                     /locus_tag="ACIYSB_003035"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGC5430031.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21508..22233
                     /gene="gltL"
                     /locus_tag="ACIYSB_003036"
     CDS             21508..22233
                     /gene="gltL"
                     /locus_tag="ACIYSB_003036"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGC5430032.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22351..23286
                     /gene="rihA"
                     /locus_tag="ACIYSB_003037"
     CDS             22351..23286
                     /gene="rihA"
                     /locus_tag="ACIYSB_003037"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGC5430033.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTSVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23370..25040
                     /gene="hscC"
                     /locus_tag="ACIYSB_003038"
     CDS             23370..25040
                     /gene="hscC"
                     /locus_tag="ACIYSB_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGC5430034.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(25100..26551)
                     /gene="djlC"
                     /locus_tag="ACIYSB_003039"
     CDS             complement(25100..26551)
                     /gene="djlC"
                     /locus_tag="ACIYSB_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HGC5430035.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPIALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWLIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQTEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPFLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIVTRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26548..27255)
                     /gene="ybeU"
                     /locus_tag="ACIYSB_003040"
     CDS             complement(26548..27255)
                     /gene="ybeU"
                     /locus_tag="ACIYSB_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGC5430036.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFELSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQSECPVTLKDVSDL"
     gene            27357..27911
                     /gene="ybeT"
                     /locus_tag="ACIYSB_003041"
     CDS             27357..27911
                     /gene="ybeT"
                     /locus_tag="ACIYSB_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGC5430037.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27921..29348)
                     /gene="djlB"
                     /locus_tag="ACIYSB_003042"
     CDS             complement(27921..29348)
                     /gene="djlB"
                     /locus_tag="ACIYSB_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGC5430038.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENTGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPQWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYAQSWRETEPDNEDAPYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLLVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHTLWPLYLVAKQLVQACQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29345..30052)
                     /gene="ybeR"
                     /locus_tag="ACIYSB_003043"
     CDS             complement(29345..30052)
                     /gene="ybeR"
                     /locus_tag="ACIYSB_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGC5430039.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
BASE COUNT         7504 a         7733 c         7569 g         7301 t
ORIGIN      
        1 ggcgttttgg attgtttgct gtttcttgcc ggatgcggcg tgaacgcctt atccggccta
       61 caaaagcacg caaactcaat atattgcaga gatcatgtag gcctgataag cgtagcgcat
      121 caggcaattt agcgtttgaa actgtttgtc tgttccttgc cggatgcgac gtaaacgccc
      181 catccggcat agcgaaactt aaaattactc gcctactttc gcccaggtat cacgcagccc
      241 aacggtgcgg ttaaataccg gtttttccgc cgtagaatgg cggctatcga ggcagaagta
      301 accttcacgc tcaaactgga atgctttacc cgcaaccgca tctttcagcg acggttcagc
      361 aaagccctgt ttgatcacca gcgattccgg gttaatcacc gacaggaaat catccgcagc
      421 acctgggtta ggcacgctga acagacggtc atacaaacgg atttcaaccg gcagcgcatg
      481 tgccgcgctc acccagtgaa taacaccttt gactttacga ccatctgccg gatctttgct
      541 taaggtatcg gcgtcataag tacagaagat ggtggtgata ttaccttcgg catctttctc
      601 gacgcgttct gccttaatca cataagcatt acgcagacgc acttctttac ccagcaccag
      661 acgtttgtac tgcttgttag cttcttcgcg gaaatcggcg cgatcaatcc aaatctcacc
      721 gctaaacggc acctgacggc tgcccatttc cggtttgttc ggatggttcg gcatggtaac
      781 catttcgcct tcgccctgat agttttcgat aaccagtttc accggatcga taaccgccat
      841 tgcgcgcggc gcattttcgt tgagatcttc acggatgcag gattccagcg acgccatctc
      901 aatggtgttg tcctgcttgg tcacgccgat gcgtttgcag aactcacgaa tagaagccgc
      961 agtgtaacca cgacgacgca gaccggaaat ggtcggcata cgcgggtcat cccagccttc
     1021 aacgtgcttg tcggtcacca gcaggttcaa cttacgcttg gacatcacgg tgtattccag
     1081 attcaggcgc gagaactcat actggcgcgg gtgaacagga atcgtgatgt tgtccagtac
     1141 ccagtcgtac agacgacggt tgtcctggaa ctcaagcgta cacagagagt gcgtaatacc
     1201 ttccagggca tcgctgatgc agtgggtgaa gtcgtacatc gggtagatgc accacttgtt
     1261 gccagtctgg tggtgttcag caaatttaat acggtacagc accggatcgc gcatcacgat
     1321 aaacggtgaa gccatgtcga ttttcgcacg caggcaggct ttaccttctt caaaaccacc
     1381 ggcacgcatt ttttcgaaca gcgccaggtt ctcttcaacg ctgcggtcgc ggtacgggct
     1441 gtttttaccc ggttgcgtca gggtgccgcg gtattcgcgg atctgttccg gcgtcagttc
     1501 atcaacgtac gccaggcctt tattgatcag ttcgatcgca taggcgtgga gctgatcaaa
     1561 ataatcggag gagtaacgga cgttaccaga ccagtgaaaa cctaaccact ctacgtcgtt
     1621 tttgatcgac tcaacatact cgatatcttc ttttaccggg ttagtgtcgt cgaaacgcag
     1681 gttgcactgg cctttatagt cctgggcgat cccgaagttc aggcagatag atttcgcatg
     1741 gccaatatgc agatagccat tcggctccgg cgggaaacgg gtgtgtactg tggtgtgctt
     1801 accactggcc agatcttcat cgatgatctg acggataaag ttagtcgggc gggcttctgc
     1861 ctcactcatc gtggattcct caaagcgtaa acaacgtata acggcgtatg atcttataag
     1921 cgggacaggc tgacaactgt tagtttttta aaattgataa tctaacaagg attatgggga
     1981 tgattgatga aaaaaaaagc ggcagagaag atctccaccg cttgtcattg ttggatgcga
     2041 cgctcaagcg tcgcatcagg cataaagcag attacttttt gatttcatac agcggtgttt
     2101 gacccgccac aacatggccc tgagctttaa tgagcaagcc actgaaatcg tcgatattgc
     2161 tgcaaaccac cgggctaatc atcgagcggg cgttagcgtt caggtaatcc agatccattt
     2221 ccagaatcgg ttgccctgcg cttacctgcg caccctcttc caccagacgt ttaaagcctt
     2281 taccttccag cgctacggtg tcgataccca tatggacgac gatctccgcg cctttttcgg
     2341 tttccaggca gaacgcgtgg ttggtgttga agattttcac gattgtccct gcggctggtg
     2401 atacgacgat tttatctgtc ggtttcaccg ccacaccgtc acccaccgct ttgctggcga
     2461 atgcttcgtc aggaacctga tccagtgcca cgacatcacc ggtaatcggc gataccagct
     2521 ccgcgataga taccgcgttt ggtacagcct gcggttttgc tacaggcgcg gcagttgccg
     2581 gagttgcttc agctgacgca gcggctaccg gaccacgggc aacgactttc ttcatcgcat
     2641 cgccgatgga ttctgctttc gcgccaacaa tcacctgaat agtctgtttg ttcagtttca
     2701 ctaccccaga agcacccaga cgtttacaca tcgtatcgtt aacgcgggca gagtcagcca
     2761 ctgtaaggcg cagacgggtg atacaggcgt caatcgcttt caggttgtca gtgccgccaa
     2821 ccgcagcaat atagttggtt gccagttgag tcagaccttc ttcagtgttg ctgttggctt
     2881 cttcagtaac gatctcgtct tctttatctt cacgacccgg cgttttcagg ttgaacatgc
     2941 ggataaccaa actgaacacc acgaagtaga tagcgaagaa gataacgccc atcaccagca
     3001 gcatccagac gttctggctg gcggccggca ggttatacat caacgcgtag tcgatagccc
     3061 ccgcagagaa agagaagccc gcgtggatac ccagcagcgt tgccacaaac aggctgatac
     3121 cggtcagcag tgcgtgcagg aggtacagca gcggagcaag gaacatgaac aggaattcca
     3181 gcggctcagt cacaccggtc aggaacgcag taacagcaac agaaagcagc ataccgccaa
     3241 ccatcggacg acgctctttc ggtgctgcga agtacatcgc cagcgccgca cccggcagac
     3301 cgaacatcat gatcgggaag aagccggaca tgaacatccc cgcggtgccg tcaccggcat
     3361 agaagcggtt aatgtcaccg tggaaaaccg tacccgccgc gttggtgaat tcaccaatct
     3421 ggaaccaggc gatggtgttc agtacctgat gcagaccggt tgggatcagc agacggttga
     3481 tgaaaccaaa gataccggaa cccagcgcgc ccgcagaaac gatccactcg ccgcctgcat
     3541 ggatagcgtg ctgtaccggc ggccagacgt aaccaaaaat ggccgccagc accaggcaga
     3601 agaatccggt ggcaatcggc acaaagcgtt tgccgccgaa gaagctcagg aagtccggca
     3661 gtttaatatc ggaccaacgg ttataggctg cgccaccaac cagaccggta atgatacccg
     3721 ccagtacacc catgttaatt tctgggttga tggtcaccat cgctttggtt aacacaaagt
     3781 aacctaccgc acccgccagc gccgccgcac cagcgctgtc tttcgaccag ctggatgcca
     3841 caccgatggc gaagattaat gcgaggttat caaaaatcgc accgcccgcc tgggcaataa
     3901 acgcaacgtt aagtaaatct ggctgaccga atcgcagcaa cagtgccgcc accggcagca
     3961 ccgcgatagg gagctgtaac gccctaccga gtcgctggaa aaaacctaaa atattcatct
     4021 tattccccct acgagaaccc tatttggctc gtttcaagcc gtatttttat tttgctgcaa
     4081 attgtactgc cgatgttctg taatcagatt gttagatcat ctgctacaga gtgtgtgaaa
     4141 atttaattcg tatcgcaaat taaacgcgtg tcttttgtga gttttgtcac caaatatcgt
     4201 tatcatcact cccttttact ggctaaacca gaaaacttat tttatcattc aaaaaatcag
     4261 gtcggattga cgcctgtctg cgcaaatcca ggttacgctt aaagatgcct aatccgccaa
     4321 cggcttacat tttacttatt gaggtgaata atgagactga tccccctgac taccgctgaa
     4381 caggtcggca aatgggctgc tcgccatatc gtcaatcgta tcaatgcgtt caaaccgact
     4441 gccgatcgtc cgtttgtact gggcctgccg actggcggca cgccgatgac cacctataaa
     4501 gcgttagtcg aaatgcataa agcaggccag gtcagcttta agcacgttgt caccttcaac
     4561 atggacgaat atgtcggtct gccgaaagag catccggaaa gctactacag ctttatgcac
     4621 cgtaatttct tcgatcacgt tgatattcca gcagaaaaca tcaaccttct caacggcaac
     4681 gccccggata tcgacgccga gtgccgccag tatgaagaaa aaatccgttc ttacggaaaa
     4741 attcatctgt ttatgggcgg tgtaggtaac gacggtcata ttgcatttaa cgaaccggcg
     4801 tcttctctgg cttctcgtac tcgtatcaaa accctgactc atgacactcg cgtcgcaaac
     4861 tctcgtttct ttgataacga tgttaatcag gtgccaaaat atgccctgac tgtcggtgtt
     4921 ggtacactgc tggatgccga agaagtgatg attctggtgc tgggtagcca gaaagcactg
     4981 gcgctgcagg ccgccgttga aggttgcgtg aaccatatgt ggaccatcag ctgtctgcaa
     5041 ctgcatccga aagcgatcat ggtgtgcgat gaaccttcca ccatggagct gaaagttaag
     5101 actttaagat atttcaatga attagaagca gaaaatatca aaggtctgta attgttatcc
     5161 ctgccctggc tccttgctca gggcaatatt ttttaaaatc gggggtcaga atgtatgcat
     5221 taacccaggg ccggatcttt accggccacg aatttcttga tgaccacgcg gttgttatcg
     5281 ctgatggcct gattaaaagc gtctgtccgg tagcggaact gccgccagag atcgaacaac
     5341 gttcactgaa cggggccatt ctctcccccg gttttatcga tgtgcagtta aacggctgcg
     5401 gcggcgtaca gtttaacgac accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc
     5461 agaaagccaa tgagaaatca ggctgtacta actatctgcc gacgcttatc accaccagcg
     5521 atgagctgat gaaacagggc gtgcgcgtta tgcgcgagta cctggcaaaa catccgaatc
     5581 aggcgttagg tctgcatctg gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata
     5641 atccgaattt tgtgcgtaag cctgatgccg cgctggtcga tttcctgtgt gaaaacgccg
     5701 acgtcattac caaagtgacc ctggcaccgg aaatggttcc tgcggaagtc atcagcaaac
     5761 tggcaaatgc cgggattgtg gtttctgccg gtcactccaa cgcgacgttg aaagaagcaa
     5821 aagccggttt ccgcgcgggg attacctttg ccacccatct gtacaacgcg atgccgtata
     5881 ttaccggtcg tgaacctggc ctggcgggcg cgatcctcga cgaagctgac atttattgcg
     5941 gtattattgc tgatggcctg catgttgatt acgccaacat tcgcaacgct aaacgtctga
     6001 aaggcgacaa actgtgtctg gttactgacg ccaccgcgcc agcaggtgcc aacattgaac
     6061 agttcatttt tgcgggtaaa acaatatact accgtaacgg actttgtgtg gatgagaacg
     6121 gtacgttaag cggttcatcc ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt
     6181 gcggtatcgc actggatgaa gtgctacgta tggcgacgct ctatccggcg cgtgcgattg
     6241 gcgttgagaa acgtctcggc acactcgccg caggtaaagt agccaacctg actgcattca
     6301 cacctgattt taaaatcacc aagaccatcg ttaacggtaa cgaggtcgta actcaataag
     6361 agaaagtatg acaccaggcg gacaagctca gataggtaat gttgatctcg taaaacagct
     6421 taacagcgcg gcggtttatc gcctgattga ccagtacggg ccaatctcgc ggattcagat
     6481 tgccgagcaa agccagcttg cccccgccag cgtaaccaaa attacgcgtc agcttatcga
     6541 acgcgggctg atcaaagaag ttgatcagca ggcctccacc gggggccgcc gcgctatctc
     6601 catcgtcacc gaaacccgca atttccacgc aatcggcgta cggcttggtc gtcatgacgc
     6661 caccatcact ctgtttgatc tcagcagcaa agtgctggca gaagaacatt acccgctgcc
     6721 ggaacgtacc cagcaaacgc tggaacatgc cctgttgaat accattgctc agtttattga
     6781 tagctaccag cgcaaactgc gcgagctgat cgcgatttcg gtgatcctgc cagggcttgt
     6841 tgacccggac agcggcaaaa ttcattacat gccgcatatt caggtagaaa actgggggct
     6901 ggtagaagct ctggaagaac gttttaaagt gacctgtttc gttggtcacg atatccgtag
     6961 tctggcgctg gcggagcact acttcggtgc aagtcaggat tgcgaagact ccattctggt
     7021 gcgtgtccat cgcggaaccg gggccgggat tatctctaac gggcgcattt ttattggccg
     7081 caacggcaac gtcggtgaaa ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca
     7141 ctgcggcaac tttggctgcc tggaaactat cgctgccaac gctgccattg aacaacgggt
     7201 gttgaatctg ttaaagcagg gctaccagag ccgcgtgccg ctggacgact gcaccatcaa
     7261 aactatctgc aaagccgcga acaaaggcga tagtctggcg tcggaagtaa ttgagtatgt
     7321 cggtcgtcat ctgggtaaaa ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat
     7381 tgttattgcc ggtgaaatca ccgaagccga taaagtgctg ctccctgcta ttgaaagctg
     7441 cattaatacc caggcgctga aggcgtttcg cactaatctg ccggtggtac gttctgagct
     7501 ggatcaccgc tcggcaatcg gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat
     7561 tttgctccag catttgctgg aaaattaatg tgcttttata gtggcgctta ttgttgtcaa
     7621 tattctgggt agtccatgac cattaaaaat gtaatttgcg atatcgacgg cgtgctgatg
     7681 cacgataacg tcgccgtacc gggtgcagcg gaatttttgc acgggattat ggataaaggc
     7741 ctgccgctgg tgttgctgac caactatcct tcgcagactg ggcaagatct ggcgaaccgc
     7801 tttgccaccg caggtgtcga tgtacctgac agcgtgtttt atacctctgc gatggcgact
     7861 gccgatttcc tgcgtcgcca ggaaggcaag aaagcgtatg tggtgggcga aggcgcactg
     7921 attcatgaac tgtacaaagc cggtttcact attaccgatg tgaaccctga tttcgtgatt
     7981 gttggcgaaa cgcgttccta caactgggac atgatgcata aagcagccta tttcgtcgct
     8041 aacggtgcac gttttatcgc caccaatccg gacacccacg ggcgcggttt ttatcccgct
     8101 tgtggcgcgt tgtgtgcagg gattgagaaa atctccgggc gcaaaccgtt ctatgttggt
     8161 aagcccagcc cgtggatcat ccgcgcagca ttaaacaaaa tgcaggcgca ttcggaagaa
     8221 acggtgattg tcggcgataa cctgcgtacc gatattctgg ccggcttcca ggcaggtctg
     8281 gagacgattc tggtgctttc tggtgtttcg tcgctcgacg atatcgacag tatgcctttc
     8341 cgccccagct ggatttaccc gtcagtcgct gaaatcgacg ttatctgaat agcaaccacg
     8401 tctgcggacg tggttggctc tgatgacaaa cgccaaactg cctgatgcgc tacgcttatc
     8461 aggcctacgc agctcctgca atatattgaa ttttcatgct tttgtaggcc ggataaggcg
     8521 ttcacgccgc atccggcatg aacaaatcgc actttgtcag caatttggcc cccgtaaaat
     8581 ataagcattc aataaaaccc gctaaaaatt atcgattcat caataaatat atcctcacac
     8641 cagacctttt ttcaccaatc gacaatcacc attacgtttt ttattttttc accgccaaat
     8701 cgcttgcgca cagtgctgct ttgcggcatt tttttaaaca agcaaacaca acaagcaaca
     8761 aataccaggt taacggagaa ggttatgtgt tcaatttttg gcgtattcga tatcaaaaca
     8821 gacgcagttg agctgcgtaa gaaagccctc gagctgtcac gcctgatgcg tcatcgtggc
     8881 ccggactggt ccggtattta tgccagcgat aacgccattc tcgcccacga acgtctgtca
     8941 attgttgacg ttaacgcggg ggcgcaacct ctctacaacc aacaaaaaac ccacgtactg
     9001 gcggtaaacg gtgaaatcta caaccaccag gcattgcgcg ccgaatatgg cgatcgttac
     9061 cagttccaga ccgggtctga ctgtgaagtg atcctcgcgc tgtatcagga aaaagggccg
     9121 gaatttctcg acgacttgca gggcatgttt gcctttgcac tgtacgacag cgaaaaagat
     9181 gcctacctga ttggtcgcga ccatctgggg atcatcccac tgtatatggg gtatgacgaa
     9241 cacggtcagc tgtatgtggc ctcagaaatg aaagcgctgg tgccagtttg ccgcacgatt
     9301 aaagagttcc cggcggggag ctatttgtgg agccaggacg gcgaaatccg ttcttactat
     9361 catcgcgact ggttcgacta cgatgcggtg aaagataacg tgaccgacaa aaacgagctg
     9421 cgtcaggcac tggaagattc agttaaaagc catctgatgt ctgatgtgcc ttacggtgtg
     9481 ctgctttctg gtggtctgga ttcctcaatt atttccgcta tcaccaagaa atacgcagcc
     9541 cgtcgcgtgg aagatcagga acgctctgaa gcctggtggc cgcagttaca ctcctttgct
     9601 gtaggtctgc cgggttcacc ggatctgaaa gcagcccagg aagtggcaaa ccatctgggc
     9661 acggtgcatc acgaaattca cttcactgta caggaaggtc tggatgccat ccgcgacgtg
     9721 atttaccaca tcgaaactta tgatgtgacc actattcgcg cttcaacacc gatgtattta
     9781 atgtcgcgta agatcaaggc gatgggcatt aaaatggtgc tgtccggtga aggttctgat
     9841 gaagtgttcg gcggttatct ttacttccac aaagcaccga atgccaaaga actgcatgaa
     9901 gagacggtgc gtaaactgct ggccctgcat atgtatgact gcgcgcgtgc caacaaagcg
     9961 atgtcagcct ggggcgtgga agcacgcgtt ccgttcctcg acaaaaaatt ccttgatgtg
    10021 gcgatgcgta ttaacccaca ggataaaatg tgcggtaacg gcaaaatgga aaaacacatc
    10081 ctgcgtgaat gttttgaagc gtatctgcct gcaagcgtgg cctggcggca gaaagagcag
    10141 ttctccgatg gcgtcggtta cagttggatc gacaccctga aagaagtggc tgcgcagcag
    10201 gtttctgatc agcaactgga aactgcccgc ttccgcttcc cgtacaacac gccaacctct
    10261 aaagaagcgt acttgtatcg ggagatcttt gaagaactat tcccgcttcc gagcgccgct
    10321 gagtgcgtgc cgggcggtcc ttccgtcgct tgttcttccg ctaaagcgat cgaatgggat
    10381 gaagcgttca agaaaatgga cgatccgtct ggtcgcgcgg ttggtgttca ccagtcggcg
    10441 tataagtaag aaatgcgaaa cagccccggt gaaatccggg gctgtttcag ttattgaggt
    10501 gcttgttgat tctgatatgc agtaaggaaa tgttccgggt ttgccacatc actgtttgac
    10561 aactcatcac tgtaggccag tatttcatag attaatttcc caccactatt gttaaattcc
    10621 gaatttcatc ctgtatgaca aaagaatggc tgtagggtgg gaaaaaacac aaagtaaaca
    10681 ataattgacg aatatagcgc cacgctgttc gcaacctaac caaacagtca ctttcgagca
    10741 attttccttg aaaaagaggt tgacgctgca aggctctata cgcataatgc gccccgcaac
    10801 gccgataagg tatcgcgaaa aaaaagatgg ctacgtagct cagttggtta gagcacatca
    10861 ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc caccatcttt ttttgcggga
    10921 gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg tgcgagttca
    10981 agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat cgccaagcgg
    11041 taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc ccagccatct
    11101 tcttcgagta agcggttcac cgcccggtta ttggggtatc gccaagcggt aaggcaccgg
    11161 tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatcga agaaacaatc
    11221 tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga
    11281 atcccgtcgt agccaccaaa ttctgaatgt gtcgaatatg ttcggcaaat tcaaaaccaa
    11341 tttgttgggg tatcgccaag cggtaaggca ccggattctg attccggcat tccgaggttc
    11401 gaatcctcgt accccagcca atttattcaa gacgcttacc ttgtaagtgc acccagttgg
    11461 ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc
    11521 gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc tgcgttcatt
    11581 caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga cgctgccgcg
    11641 tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca accctaacgc
    11701 atatttcagc gcctgacgtt tcaacacgcc agcacgctcc gccgccatta acccgagatt
    11761 acgcataaaa cgcagtggtg gcagattatt gctgaatccg gcataaaaca gatccatacc
    11821 gctttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga ggacaggata
    11881 actggcccac gcttcgccgt agctgcgggc gttgaccaga acatcaatca gggcatcgac
    11941 atcacgataa ccaagattca ctccctgccc cgccagcgga tggatggtat gcgcggcatc
    12001 gcccaccaac gcaagccctg gctgcacgta ctgcaacgca tggcgacgcg tcagcggaaa
    12061 cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct tcgcgatttc
    12121 cgcctggagc tgtgccatat tcatattctg caactggcga atacgcgccg gagagtcata
    12181 ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc cgtccggagt
    12241 aaattgctgc caggtgctgt cgccgggatc gttctcgcac tggacgctaa tcaacatgca
    12301 cgactgcgca tactgccatg catgaacgcc aattcccgcc atctgccgca cctgcgaatt
    12361 tgcgccgtcg gcaccaatca ccagcttcgc gcgaatcact tcaccgcctt tcagctccag
    12421 ctcctgaaga tcatcatggc gatgcagcgc aatcagcgag cctggcacac gtaacgttac
    12481 tttcggatgc gcttccagcg cctgccacaa cgcctgttgc aggacagtgt tttccaccat
    12541 atagccaagc agcggtagct taagttcagc ggcgtcaaac accacatgcg ccgtttccca
    12601 ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg catcccagac
    12661 ccctaaccct ttaagcaatg ataccgaagc cgcgctgatc gccgagatcc gcacgtccgg
    12721 ttggctatca gcgacaaacg gcgctggttc tgcgtgctcg atcaccgtta ccgcaaatcc
    12781 gtgctgtgcc agccccagcg ccagtgcgcc gccgaccatt cctccgccga caatggcaat
    12841 ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt aagtttacag
    12901 gatttttacc cctaagggct gataacgctc atactggtca caacggcagc aaagcattac
    12961 actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga ccaaaaaact
    13021 ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga tggccgatct
    13081 gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg atgtgctgct
    13141 gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc agttgggtcg
    13201 ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg gctgcgtggc
    13261 atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta tttttgggcc
    13321 gcaaacgctg caccgtctgc cggagatgat caactccgtg cgtggcgacc gcagcccggt
    13381 tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac cgcgcgccga
    13441 agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca cctactgcgt
    13501 ggtgccttac acccgtggtg aagaggtaag ccgtccgtcc gacgatattc tgtttgagat
    13561 tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga acgtgaacgc
    13621 ctggcgtggt gaaaactacg acggcaccac cggatcgttt gccgatctgc tgcgtctggt
    13681 tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga tcgaattcac
    13741 cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc tgcatctgcc
    13801 ggtacagagc ggttccgatc gcattctgaa cctgatgggc cgtacccata cggcgctgga
    13861 gtacaaagcg atcatccgta aactgcgtgc ggcgcgtcca gatattcaga tcagttctga
    13921 tttcatcgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga tgaagctgat
    13981 tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc cgggtacacc
    14041 agccgccgat atggttgatg atgttccgga agaagagaag aagcagcgtc tgtatattct
    14101 gcaagagcgc attaatcagc aagcgatggc gtggagccgc cggatgctcg gcaccaccca
    14161 gcgtattctg gtagaaggta catcgcgtaa gagcatcatg gagctttccg ggcgtacgga
    14221 aaataaccgc gtggtcaact tcgaaggcac gccggatatg atcggtaaat tcgtcgatgt
    14281 agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta ctgaagatga
    14341 aatgggtctg cgcgtggcag aaacaccgga atcagtgatt gcccgtaccc gcaaagaaaa
    14401 cgaccttggc gtgggttatt atcagccgta attctcaggc ctgccgttcc ggtgggcctt
    14461 gttttttctc tctttatccc catctttatt gcaatgcttg cgcctttttc ctgtggcgtg
    14521 aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga taagaggaac
    14581 ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaatgc gcgtctgttg
    14641 agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct cggcatcgag
    14701 atcaatcgcc gcgataacca ctttaaactg accggccgtc cgatttgcgt caccgctgcg
    14761 gcagacattc tgcgtagcct gtatgtcgat actgccccga tgcgcggtca gattcaggat
    14821 atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gggtactgga gcaaagcgcg
    14881 gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg cgtaattaag
    14941 ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga cattaccttc
    15001 ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc agttgatgcc
    15061 ctggagcgtc aggaaattcg ccgtattctg ctgactcgtc cggcagtaga agccggtgag
    15121 aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct gcgcccgctg
    15181 tacgacgcgc tgtttgaaat gctgggcttt gagaaagtcg agaaactgat tgagcgcaac
    15241 gttattgaag tcgcaccgct ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc
    15301 attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct gacccgtatc
    15361 ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct gccgcgtaat
    15421 actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga gatcagcttt
    15481 aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat cgttaacgcc
    15541 tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcagc agaacgcaag
    15601 cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg gcatgtgaag
    15661 ataattccgg gttaccggaa gagagccagt ttcagacatg gctgaatgcg gtgatcccgc
    15721 agtttcagga agaatcggaa gtgacgattc gcgtggtcga taccgccgaa agccacagtc
    15781 tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc ccgtttgaag
    15841 tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt caggtggttg
    15901 agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat atggtggtgc
    15961 acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca gaagaaatgg
    16021 aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac attgccgaga
    16081 aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga ttaacgacgg
    16141 cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca attcacacag
    16201 tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc aacttttcca
    16261 cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg ggcagaacga
    16321 ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg cagaccaacg
    16381 cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca accagacgct
    16441 ggacgaatgt cttgatgtca tcatcgagtc cgcccactca cgtttcccgg tgattagcga
    16501 agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt ttatgcgcag
    16561 cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag
    16621 taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt
    16681 tattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc tggaactgat
    16741 tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc gtcagctgag
    16801 tcgtcatacc tggaccgtac gcgcactggc ttccattgaa gacttcaacg aagcgttcgg
    16861 cacccacttt agcgatgaag aagtcgacac tatcggtggt ctggtgatgc aggcatttgg
    16921 gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca aagtggcgat
    16981 ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact caccccagcc
    17041 gaagctggat gaataaaacc gaaactggat agataactac atggcttttg cctcattaat
    17101 tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg gaacgctggc
    17161 cttctctcct tacgacgtct ggcctgcggc gattatttcg ctgatggggc ttcaggcgtt
    17221 gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg gatttggcct
    17281 ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg gaatgcctgg
    17341 cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt ataccggact
    17401 gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg tagcgattgc
    17461 cgcccctgcc ctctggcaag tgaccgagtt tctgcgcggt tgggtactga ccggcttccc
    17521 gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg caccgataat
    17581 gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg cactggcgtt
    17641 ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc ttcccttccc
    17701 gctgcgttac atccagtggt ttaccccaca accggagaaa accattcagg tttcgatggt
    17761 tcagggcgat attccgcaat cgctgaaatg ggacgaaggc cagcttctta atacgctgaa
    17821 gatttactac aacgcaacgg caccgctgat gggcaaatca tcgttgatta tctggccgga
    17881 gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac tggacggtga
    17941 gttgcgtgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc tcaataagca
    18001 gaaccgctac gatacctaca acaccatcat cacgctgggt aaaggtgcgc cgtacagcta
    18061 cgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt ttgtcccgct
    18121 ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg
    18181 tgggccatat atccagccgc cgctgtcggc aaatggtatt gagcttactg cggctatttg
    18241 ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata ccgactatct
    18301 gctgactatc tccaacgatg cgtggtttgg taaatctatt ggtccatggc aacacttcca
    18361 gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca ccaacaacgg
    18421 cattacggcg gtgattggcc cgcagggtga gattcaggcg atgatcccgc agttcacccg
    18481 cgaggtgtta accactaacg tgacgccgac caccggactc acaccatacg cacgtaccgg
    18541 caactggccg ctgtgggtgt tgacggcatt gtttggtttt gctgctgtgt tgatgagtct
    18601 gcgtcagcga cgtaaataat cccttcttat cgtgccggaa tacatcccat atttcggcac
    18661 gctcaccgga tgcggcatca gtgcattatc cggataacaa tattctccta tccaactttg
    18721 gcacatctat tgctttgtta tacaaggcaa accctgaatc agcatcagta caacctagtt
    18781 gcaccacagt aatccagcgg tagcactaaa agagtgcatt ttaagaattt tgcttcggaa
    18841 tggtgcggcg catctcgcaa aaacaaacaa taaaacagca atatctttac atttagccaa
    18901 actaaatgtt acctaacagg cacaacactg cacaataaag ttgcagacga taacaacaca
    18961 aacactcaca acgggtatcc atgcgttctt aacgcagaag ataaaggagt tggatatgca
    19021 attacgtaaa cctgccacag caatcctcgc cctggcgctt tccgcaggac tggcacaggc
    19081 agatgacgcc gccccggcag cgggcagtac tctggacaaa atcgccaaaa acggtgtgat
    19141 tgtcgtcggt caccgtgaat cttcagtgcc tttctcttat tacgacaatc agcaaaaagt
    19201 ggtgggttac tcgcaggatt actccaacgc cattgttgaa gcagtgaaaa agaaactcaa
    19261 caaaccggac ttgcaggtaa aactgattcc gattacctca caaaaccgta ttccactgct
    19321 gcaaaacggc actttcgatt ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa
    19381 acaggcggct ttctctgaca ctattttcgt ggtcggtacg cgcctgttga ccaaaaaggg
    19441 tggcgatatc aaagattttg ccaacctgaa agacaaagcc gtagtcgtca cttccggcac
    19501 tacctctgaa gttttgctca acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat
    19561 cagcgccaaa gatcacggtg actctttccg caccctggaa agcggtcgtg ccgttgcctt
    19621 tatgatggat gacgctctgc tggccggtga acgtgcgaaa gcgaagaaac cagacaactg
    19681 ggaaatcgtc ggcaagccgc agtctcagga ggcctacggt tgtatgttgc gtaaagatga
    19741 tccgcagttc aaaaagctga tggatgacac catcgctcag gtgcagacct ccggtgaagc
    19801 ggaaaaatgg tttgataagt ggttcaaaaa tccaattccg ccgaaaaacc tgaacatgaa
    19861 tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa ccgaatgaca aggcactgaa
    19921 ctaattacaa gaaccagggg cggaaattcc agccctctcg attgttacgt agcacggaca
    19981 gactatacgc ctgatggtcg ttccccatcg ggcctgaaaa ccgcaatacg ctgggtaaca
    20041 atcttcgagg gtagcagtta acgctgctac cctctttttt ctggagtaga tttatgtcta
    20101 tagactggaa ctggggtatt tttttacaac aagccccgtt cggcaacacc acctatcttg
    20161 gttggatctg gagtggtttt caggtgacga tcgctttgtc gatctgcgcc tggattatcg
    20221 ctttcctcgt cggttcattt ttcggcattt tacgtaccgt tccaaaccgt tttctctctg
    20281 gtctgggtac gttgtatgtc gaactgttcc gcaacgtgcc gttgattgtg caattcttta
    20341 cctggtatct ggtgatcccg gagctgctgc cggagaaaat cggcatgtgg tttaaggccg
    20401 agctggatcc caatattcag tttttccttt catccatgct ctgcctgggg ctgtttaccg
    20461 ccgcccgtgt ttgcgaacag gtacgcgccg cgattcagtc gctgccgcga gggcaaaaaa
    20521 atgccgcgct ggcgatgggc ctgacgctac cgcaggctta tcgatacgta ctgctgccta
    20581 atgcttatcg cgttatcgtc ccgccgatga cctcagagat gatgaacctg gtgaaaaact
    20641 ccgccatcgc ctcgactatc ggtctggtgg atatggcggc gcaggcgggt aaattgctcg
    20701 attactcagc ccatgcctgg gagtcgttta ccgccattac gctggcgtat gtgttgatta
    20761 acgcttttat catgctggtg atgacgctgg ttgaacgtaa agttcgcctg cctggcaaca
    20821 tggggggcaa ataatgtacg agtttgactg gagttccatt gtcccttccc tgccatatct
    20881 gctcgacggg ctggtgatca ccctgaaaat caccgtcacg gcggtagtga ttggtatttt
    20941 gtggggcacg atgctggcgg tgatgcgttt atccagcttt gcgcccgtgg cgtggtttgc
    21001 caaagcctac gttaacgtat tccgctcgat tcctttagtt atggttttgc tgtggtttta
    21061 cctgatcgtg ccgggttttc tgcaaaacgt gctgggatta tcgccaaaaa atgatatccg
    21121 tttaatttcg gcgatggtgg cgttttcgat gtttgaagcg gcctactatt cagagattat
    21181 ccgtgctggt attcaaagta tttctcgtgg tcaatcgagc gccgcgctgg cgttgggaat
    21241 gactcactgg cagtcgatga aactgattat tctgccgcag gcgttccgcg cgatggtgcc
    21301 gctgctgctc actcagggca tcgtactgtt ccaggatacc tcactggtgt atgtgttatc
    21361 cctggccgat ttcttccgta ccgcctcaac cattggtgag cgtgatggta cgcaggttga
    21421 gatgatcctg tttgccggat ttgtttattt cgttattagt cttagcgcgt cgttgttggt
    21481 cagctacttg aaaagaagga cagcataatg attaccctga aaaatgtttc aaaatggtat
    21541 ggtcactttc aggtgctgac cgactgctca accgaagtga aaaaaggcga agtggtggtg
    21601 gtttgcggcc cgtctggttc cggcaaatca acgctgatta aaaccgtcaa cggcctcgaa
    21661 ccggtgcagc aaggtgaaat caccgtcgat ggtatcgtgg ttaacgacaa gaaaaccgat
    21721 ctggcaaagc tgcgttcccg cgtcgggatg gtattccagc atttcgagct gttccctcat
    21781 ctgtcgatta tcgaaaacct gaccctggcg caggtgaaag tgcttaaacg cgataaagcg
    21841 ccggctcgtg aaaaagccct gaaactgctg gagcgtgtcg ggctttctgc tcacgccaat
    21901 aagtttccgg cgcagctttc cggcggtcag cagcagcgtg tggcaatcgc tcgcgcgttg
    21961 tgtatggatc ctattgcgat gctgtttgac gaaccgacat cggcgctgga tccggagatg
    22021 atcaacgaag tactggacgt gatggtggaa ctggcgaacg aaggaatgac catgatggtg
    22081 gtgacccacg aaatgggctt tgcccgtaaa gtggcgaatc gggtgatctt tatggacgag
    22141 ggtaaaattg tcgaagactc gccgaaagac gctttcttcg atgatccgaa atcggaccgc
    22201 gcaaaagact tcctcgcgaa aatcctgcat taatcactct ggcgcgcata cccaattgcg
    22261 cgccatctca cgctttatcg tttcgcccct cgtatctgtt acatcctggc gttacccttg
    22321 tcgcaaagaa gcacacaaca aggagcaaca atggcactgc caattctgtt agattgcgac
    22381 ccaggtcatg acgacgctat cgcaatagtt ctcgccctcg cctcaccaga gcttgatgtc
    22441 aaagcaatta cgtcttccgc cggaaaccag acaccagaaa aaaccttacg caatgttctg
    22501 cgtatgctga ccttgcttaa tcgcaccgat attccggtag caggcggcgc ggtaaaaccg
    22561 ttaatgcgtg agttgattat cgcggacaat gtgcacggcg aaagcggtct cgacggcccg
    22621 gcattaccgg aaccgacatt cgcaccgcaa aactgtacgg cggtagagct gatggcgaaa
    22681 acgctgcgtg aaagtgcgga acctgtcacc attgtgtcta ccggaccgca aactaacgtt
    22741 gccttgctgc tcaatagcca tccggaactg catagcaaaa ttgcccgtat cgtgattatg
    22801 ggtggcgcta tggggctggg taactggacg cctgcggctg aatttaatat ttacgttgac
    22861 ccggaagcgg cagaaattgt cttccagtca gggatcccgg tggtgatggc cggtctggat
    22921 gttactcata aagcacaaat ccacgttgaa gacaccgagc gtttccgcgc gattggtaac
    22981 cctgtttcaa ccattgttgc cgaactgctg gatttcttcc tcgaatatca taaagacgaa
    23041 aaatggggct ttgtcggcgc accactgcat gacccatgca ccatcgcctg gctgttgaaa
    23101 ccggagttat ttacctctgt tgagcgctgg gttggcgtgg aaacacaggg gaaatatacc
    23161 cagggtatga cggttgttga ttattattat ctgacaggca ataaaccgaa tgccaccgta
    23221 atggtcgatg ttgatcgtca gggctttgtt gatttactgg ctgatcgtct gaaattttac
    23281 gcttaacaca cgtcattgat taagatcacc acgcgttacc tgggtggtct taatctgttt
    23341 atctcttttt ctttcaagat ataaatgcaa tggataatgc agaactcgcc attggtatcg
    23401 atctcggtac taccaatagt ttaattgccg tctggaaaga cggtgccgcg caattaattc
    23461 caaataagtt cggtgaatat ttaacaccat ccataattag catggatgaa aataatcata
    23521 ttttagtcgg aaaaccggct gtatcacggc gtacttcgca tccggataaa acggcagcgt
    23581 tatttaaacg tgcaatgggc agtaatacca actggcggtt aggcagcgac acatttaacg
    23641 cgccagaact gtcctctttg gtattacgct cattaaaaga agatgccgaa gaatttctgc
    23701 aacgtccgat taaagatgtg gtgatctccg ttccggctta tttcagcgat gaacaacgca
    23761 agcatacccg tttagcagcg gagttagccg ggttaaatgc ggtacgctta attaatgaac
    23821 ccacagcagc tgcgatggcg tatggcctgc atacccaaca aaatacccgt tcgctggtgt
    23881 ttgatctcgg tggcggcacg tttgacgtta cggtgcttga gtacgccacg ccggtgattg
    23941 aagttcacgc ctccgctggc gacaactttc ttggtggcga agattttacc catatgctgg
    24001 tcgatgaggt tttaaaacgc gcggatgtcg ccaggaccac gcttaacgag agtgaactgg
    24061 cagccttgta cgcctgtgtg gaagcggcaa aatgtagcaa tcaatcgcca ttgcacattc
    24121 gctggcagta tcaggaagaa acgcgggaat gcgaatttta cgagaacgaa ctggaagatt
    24181 tgtggttgcc gctgctcaat cgcttgcgag tgccgattga acaggcgttg cgcgatgcgc
    24241 gtctgaagcc gagtcaaatc gacagtctgg tgctggttgg cggcgcgtca caaatgccgc
    24301 tggtgcagcg aatcgccgtg cgtctgtttg gcaaattacc gtatcaaagt tacgatccga
    24361 gcaccattgt cgcgctgggc gcagcaatcc aggccgcctg ccgcttacgc agtgaagata
    24421 ttgaagaggt aatcctcact gatatttgcc cttactcgtt gggcgttgaa gttaaccgcc
    24481 agggcgtttc cggcattttc tcgccgatta ttgaacgaaa caccactgtg cccgtgtcgc
    24541 gtgtagaaac ttattcaacc atgcacccgg aacaggattc aattacggtt aacgtctatc
    24601 agggagaaaa ccacaaagtt aaaaacaaca ttctggtgga atccttcgat gtgccgttga
    24661 agaaaaccgg ggcttatcag tcgattgata ttcgctttag ttatgatatc aacgggttgc
    24721 ttgaagttga cgtgcttctg gaagacggca gcgttaagtc cagagtgatt aaccacagcc
    24781 cggtaacatt gagcgcgcag cagattgaag agagtcggac gcggttatcc gcattgaaaa
    24841 tttatccgcg cgatatgctc atcaatcgca cctttaaagc caaactggaa gagttgtggg
    24901 cgcgggcgct gggtgacgag cgagaagaga tcggccgggt gatcaccgat tttgatgcgg
    24961 cgttgcagtc aaacgatatg gcccgcgtgg atgaagttcg gcggcgggcg agcgattatt
    25021 tagccattga gatcccataa acacggctgc cggatgcggc gtaaacgctg catccggcat
    25081 cggcgcaaaa aacacatcgt cagctaaaca acatcgcgat tttgccgaga gcataaccta
    25141 tcaccagcac cgttaaactg attttgataa tgctggtaat gctctgtggt ttttcaccct
    25201 catcattttt ttcttcatga ttttcaggtg ttggctcatc gacatcttca tcctccggct
    25261 cgagtttcac tttcgtcaaa agcatccggg tgacaatggc ctctaatgga tgagcatctc
    25321 cgccttccag ccttgtatta agacgggcca gcatctcttg tttcggaaac gcttcaagta
    25381 ctacctgttc cgctacccga tacaaatcca ggaacggtga atcttgctct gtctcgctac
    25441 cgactctcga tttccaccac gcccatgctt tgcgcttgaa gaataacgac tcaataaacg
    25501 attgacctaa cggcgataat tttccgctat gtaaaatctc cgcccataac agccgcgtcc
    25561 gggcgctctc accaggaaca tatttcagcg gttgaccttg cgcatctacc atgctccgcg
    25621 cttcgataac catcactaat aacgcgaaat aatccgggca atgacgcttg ctccactgca
    25681 acaacaaatt atcagcttgt gtagatgggt aactttccca gtacgcgcag agatcggcaa
    25741 gcaggctttc cccctcgccg cagtacaaac gttgtgcaca caagtaatac cgggcatctt
    25801 cgctttctgg ttcttccgtt tgccactgtt gagcgacagg aatgagttcc gccatacctg
    25861 tttgcaccga gctgtaccag cgcaacagtt tgcgatgtaa ctttgcatca tcaggaatta
    25921 accacggacc ttccatcgcc aaaaacgcag tgacatagtc aggatggtaa cggcagcaac
    25981 gttccagcgc gaagtaatac tcaacagtct ggttctgcaa cgcaatggga tattccagaa
    26041 ggctaaggaa atcgaaacaa tcaccagcct gaatggcctc aagaaaagcc tccctttctt
    26101 cctcatccat tccttcgctg tcattaaatg aatgccagtt caaacgctct gccagcaaag
    26161 aagcacaatt taatgaaaga tatcgcgctt ctatggcgat tgcacacagc ggccagcgta
    26221 attgatcgac atcctccatg ttccaggtat ttaattgctg aataaatttc tgccaggcgg
    26281 aaggctgaaa acgatcactt tctgaatcca gtaatgtcct gaatgcacgt agtatttcat
    26341 gttcagcggc agcatctttt tcttcatcat ctgcttcctc gacaggattt accgccagtc
    26401 gcagggcctc ttcataggcc tggcgtaatg ctttaaaccc ttgcggatcg gtttcgggat
    26461 gacacaacgg taagcgagca agataagcct ggcggataat gtctatttgc gtcgtgcttt
    26521 caatttcgag gatttgccaa catgttttca taggtcgctc acatccttaa gcgttaccgg
    26581 gcattcagac tggacagtta atggctgcca gggaagcgtg gcatagaaac gatcgcttcc
    26641 cgctgccagc tcttcgaaca tacgactccc ggcgctatcg tatttgtaca gggttagcgc
    26701 ctcgcgtagc gaagtgttgt cttcgcactg agaagattgc cagtagagac gtcctaacga
    26761 ataggcggaa aaatagtgca tccagctgtg atagtaatgg tgagctcgta catagacgcg
    26821 cgattgcagc cataaactct cttcttccgt cagccacttg ttgagcacac cgacccgtga
    26881 cagaaacccc attcgaacat aatcccaggc tttgattccc ccttccccac agcacatagc
    26941 ggtttgggca acgaattggg tataaatcaa ccctctctcc gagccaccag caattaatgc
    27001 tttccactct tcaggggaag agcgatgcca tgtcagatat aagccagcta aatcattggc
    27061 atgaccatca tcagtcatct gataaatagt ctgaattaaa tcatcacgat tatttattcc
    27121 ccaggatgat gagagctcga attcttttat cccagtatac atttttggcg agtcatgcgc
    27181 gggtttgcaa ccctgattaa gtatttccat gggcgcagaa agcgcaaaaa gaagatattg
    27241 ttcttcctta ttcatttgtt aataatccct taagtgcatt aactgactac ttttttatta
    27301 ttgatgagta taaacttgac gctttcaaaa taaaaagaaa atcgaagcat tcacacatga
    27361 ataaaaaatt aatgtatata ttcgcaattt ttatagttgc agcaattacc tgtattagcc
    27421 aacccaagaa aacgacgttg cgtgataaag ccatggtgaa ttatgccttt gattatttaa
    27481 gctcaccggg cagtcttcca ttcaccacgg cagccacgga gctttccgcg attcatggtc
    27541 actcaacgtc gcaatatcgc cttggagaat tttatcttca tggtagcgac ggtaaaccac
    27601 tggattatac acaggcgaga tactggtatg agcaatcagc ggaacaggaa aatccacgcg
    27661 cgcaaagtaa actggggtgg atctacctca aaggtctggg ggtcaaaccc gacacccgta
    27721 aagcaattct ctggtataag gaagcagctg aacaagggta tgctcatgct caatatactt
    27781 taggtttgat ctacagaaat ggctcaggta ttaatgttaa ccattatgaa tctcaaaaat
    27841 ggttaaaact ggccgccaaa caacattaca aaaatgcgga aagattactt gccgggcttc
    27901 ccgcacatta acgtcgactg tcacccgaac agatggagta ttttccctat caaaccagca
    27961 aagataaaaa tatagaaaat aattttaatg atgcccagac agcctggccc accatcgtca
    28021 acgggcattg gctgcggttg cggctccggc tcaacagttt gcttctcggt aaaattcgcc
    28081 gcctgaatta ataatcgacg aataattaat gcttccagtg gacaacgatt ttctgcatca
    28141 aggcggctca caatcggcat taatgattct tctgtaggtt ggcaggcctg cactaattgt
    28201 ttggcaacta gatataatgg ccataaggta tgttctgtat cacgcgtgag tgagatccgc
    28261 ggcttacgtc ctttgtgcaa aaccatctcg accagtgaac gccccagcgc agagagtttt
    28321 tcatcgctga gtatttcgta taaatgaaaa cgcgtacggg cgctgtcgcc ggggacataa
    28381 agtagcggtt ttccctgatc gttaaccaga tcacgcgctt caatcatcat caccagtaat
    28441 gggtaataat cgacccgatg ctgacggcac cattgcaaca ttaaagcatc cgcctgggtg
    28501 gagggatact cgcgccagta gtcacaaagt tctgccaaca ggctttcgcc ttctccacaa
    28561 tagacgcgtt gcgcgtattc ataataaggc gcatcttcat tatcaggttc agtttcccgc
    28621 catgactggg cgtaatccag aagttcgggg atatccaggc gacctgcgct ataccagcgg
    28681 agtaaatttc tgtgcagttt tgcatcatcg gggataatca ctgcgcgatg ttgcgctaaa
    28741 tacaatgtca gccattgtgg atgcgatgac caaatccgag cgaacatttg gtaaaaatcg
    28801 atggtgtcat tttgcacggc aacgggcaga tgcagaatgg tctggaagtt aaaaacattc
    28861 cccttcgcaa tggcatataa aaaggattcc agttcttctt cgtctatttc cccggtattt
    28921 tcctcctgta accaccgcaa tctttctgct aataacacca cgcactcgaa ggataaatgg
    28981 gcagtgttca tggcgattgt gcacagcgac cagcgtaatt catcaatctc ctccatcgag
    29041 caataattta attgctgaat gaatcgctgc caggcggagg gcagaaaacg ttcactatca
    29101 gaggcaagta acgcacgaaa ggcgagcaga atttcatgtt ctgctacctc atattcttct
    29161 ggttgccaaa cagatttagc aggcgactgc gcaatccgta gcgcctcctc atacgcctga
    29221 cgaagttgtt taaaaccctg cggatcggtt tctggatgaa aggacggtaa cagcgccaga
    29281 taagcgcggc ggataatatc gacgtcagtc gtttcctcta tatcgaggat cttccagcaa
    29341 tttttcatag gtcgctcata tccttaagcg tttccgggca tgcagaatag taagccaatg
    29401 gttgccagga caaggtaggg taatacgcat catcactggc aaataattga taaaacatat
    29461 cattgccgga atcgtcgtat tctttacgcg ctaagaattc gcgtaataaa ggcagatgat
    29521 catcttcggg agactgccaa tattgccgac caaaggtata accggcaaag tattgccgcc
    29581 agttactata ataacgtaga gcccgtagat gaatgcgcga ctggatccat agactttctt
    29641 cttcgctcaa ccagttatta agtacgccca ttcggcttaa aaaacccatc cggacataat
    29701 cccaggcttt gattccccct tcgccacagc attcggcggt gctggcgaca aattgcgcat
    29761 aagcctgacc ttgctcattt agttcagcaa gatagtcacg ccattcacat ggcgaataac
    29821 gaaaccagcg gtgataaaac cctgccaacc gagcggcatg accgttatcg atcattcggt
    29881 gaattgtgcg cagcaggtca tcacggtcat caattcccca tgaggaagaa aggtcaaaaa
    29941 agcatgtacc taaatacatt ttgggtgatg aatgcgaagg taaacagcat tcattgcgta
    30001 tttccatcgg agtggaaagt gcaaacaata ttttttgcga ctccatgtcc atataattta
    30061 tccttaaata ttcattaaat gaatattagc cgcattgcga ggctcgt
//
LOCUS       DBBGLQ010000050        29887 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.050, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000050 DBBGLQ010000000
VERSION     DBBGLQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.050"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            211..2361
                     /gene="btsT"
                     /locus_tag="ACIYSB_003044"
     CDS             211..2361
                     /gene="btsT"
                     /locus_tag="ACIYSB_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGC5430040.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2411..2614
                     /gene="yjiX"
                     /locus_tag="ACIYSB_003045"
     CDS             2411..2614
                     /gene="yjiX"
                     /locus_tag="ACIYSB_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGC5430041.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2625..3581
                     /gene="yjiA"
                     /locus_tag="ACIYSB_003046"
     CDS             2625..3581
                     /gene="yjiA"
                     /locus_tag="ACIYSB_003046"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGC5430042.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3615..3917)
                     /locus_tag="ACIYSB_003047"
     CDS             complement(3615..3917)
                     /locus_tag="ACIYSB_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HGC5430043.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(3932..4033)
                     /locus_tag="ACIYSB_003048"
                     /pseudo
     CDS             complement(3932..4033)
                     /locus_tag="ACIYSB_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            4230..5396
                     /locus_tag="ACIYSB_003049"
     CDS             4230..5396
                     /locus_tag="ACIYSB_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGC5430044.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVNREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            5460..7079
                     /locus_tag="ACIYSB_003050"
     CDS             5460..7079
                     /locus_tag="ACIYSB_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGC5430045.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTTEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
     gene            7069..8466
                     /locus_tag="ACIYSB_003051"
     CDS             7069..8466
                     /locus_tag="ACIYSB_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGC5430046.1"
                     /translation="MGSKWKQTTLGDLALGFDGAVDGPFGSNLPAAAYTDSGYPIIRG
                     SNLSIGLDDFKDNDFVFVPEHIFQKLSRSECRAGDIIFTKKGTLGQTGLISTKHRFEK
                     YLLSSNQMRLRVDPEKALPEYVYYCVSSQKAINKIIQDSECTGVPKINLAYLKSFPIS
                     LPSLSEQQDIIDILGSLSTKITLNRQINQTLEQMSQTLFKSWFVDFDPVIDNALDAGN
                     LIPEALQSRAELRQKVRNSTDFKPLPAEIRSLFPSEFEETELGWVPKGWCIKDFSVIA
                     KLIKDNVKSKNICDDIHYIGLEHLERKHIFITNYGNGGDVSSNKSAFDKNDLLFGKLR
                     PYFHKVALAPFSGICSTDILVFRAKENIYRSLMAMYAFTDDFVAYANLRSTGTRMPRA
                     EAKDLLNYKIVLPNKNVLEKFEELLENYWIKGQLNNYENEKLTALRDTLLPKLISGEL
                     SVEDLPDLIKQTEAA"
     gene            8561..11824
                     /locus_tag="ACIYSB_003052"
     CDS             8561..11824
                     /locus_tag="ACIYSB_003052"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HGC5430047.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALCQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERNNIICISDEAHRSQVNLDQKVIVDKDSGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPVWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            12039..12380
                     /gene="symE"
                     /locus_tag="ACIYSB_003053"
     CDS             12039..12380
                     /gene="symE"
                     /locus_tag="ACIYSB_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGC5430048.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMKGCIVLTAQPPAAEESELMQSLRQVCKLSARKQKQVREF
                     IGVIAGKQKVA"
     gene            complement(12427..13590)
                     /locus_tag="ACIYSB_003054"
     CDS             complement(12427..13590)
                     /locus_tag="ACIYSB_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HGC5430049.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGDLPH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRREREEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPALPEEVLAVYRAQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(13638..14520)
                     /locus_tag="ACIYSB_003055"
                     /pseudo
     CDS             complement(13638..14520)
                     /locus_tag="ACIYSB_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(14618..14782)
                     /gene="yjiS"
                     /locus_tag="ACIYSB_003056"
     CDS             complement(14618..14782)
                     /gene="yjiS"
                     /locus_tag="ACIYSB_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGC5430050.1"
                     /translation="MEFHENKANAPFIGFVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            14959..16371
                     /gene="yjiR"
                     /locus_tag="ACIYSB_003057"
     CDS             14959..16371
                     /gene="yjiR"
                     /locus_tag="ACIYSB_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGC5430051.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAMK"
     gene            complement(16614..17558)
                     /locus_tag="ACIYSB_003058"
     CDS             complement(16614..17558)
                     /locus_tag="ACIYSB_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGC5430052.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVLLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSAEQMQALEQPLPESKRDPWIEY"
     gene            18025..19257
                     /gene="mdtM"
                     /locus_tag="ACIYSB_003059"
     CDS             18025..19257
                     /gene="mdtM"
                     /locus_tag="ACIYSB_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGC5430053.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPLQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            19298..20578
                     /gene="yjiN"
                     /locus_tag="ACIYSB_003060"
     CDS             19298..20578
                     /gene="yjiN"
                     /locus_tag="ACIYSB_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGC5430054.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVVPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            20694..21845
                     /gene="yjiM"
                     /locus_tag="ACIYSB_003061"
     CDS             20694..21845
                     /gene="yjiM"
                     /locus_tag="ACIYSB_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGC5430055.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERSGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            21855..22622
                     /gene="yjiL"
                     /locus_tag="ACIYSB_003062"
     CDS             21855..22622
                     /gene="yjiL"
                     /locus_tag="ACIYSB_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGC5430056.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMSVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            22619..22876
                     /gene="ytiA"
                     /locus_tag="ACIYSB_003063"
     CDS             22619..22876
                     /gene="ytiA"
                     /locus_tag="ACIYSB_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGC5430057.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYGVSPR"
     gene            22941..23801
                     /gene="yjiK"
                     /locus_tag="ACIYSB_003064"
     CDS             22941..23801
                     /gene="yjiK"
                     /locus_tag="ACIYSB_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGC5430058.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            23869..25047
                     /gene="yjiJ"
                     /locus_tag="ACIYSB_003065"
     CDS             23869..25047
                     /gene="yjiJ"
                     /locus_tag="ACIYSB_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5430059.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(25060..25614)
                     /gene="kptA"
                     /locus_tag="ACIYSB_003066"
     CDS             complement(25060..25614)
                     /gene="kptA"
                     /locus_tag="ACIYSB_003066"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGC5430060.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            25864..26547
                     /gene="yjiH"
                     /locus_tag="ACIYSB_003067"
     CDS             25864..26547
                     /gene="yjiH"
                     /locus_tag="ACIYSB_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGC5430061.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            26544..27005
                     /gene="yjiG"
                     /locus_tag="ACIYSB_003068"
     CDS             26544..27005
                     /gene="yjiG"
                     /locus_tag="ACIYSB_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGC5430062.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            27018..28190
                     /gene="iadA"
                     /locus_tag="ACIYSB_003069"
     CDS             27018..28190
                     /gene="iadA"
                     /locus_tag="ACIYSB_003069"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGC5430063.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            28255..29166
                     /gene="hypT"
                     /locus_tag="ACIYSB_003070"
                     /gene_synonym="qseD"
     CDS             28255..29166
                     /gene="hypT"
                     /locus_tag="ACIYSB_003070"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGC5430064.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(29159..29551)
                     /gene="iraD"
                     /locus_tag="ACIYSB_003071"
     CDS             complement(29159..29551)
                     /gene="iraD"
                     /locus_tag="ACIYSB_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGC5430065.1"
                     /translation="MMRQSRQAVLPEISGNKTSSLRKSICSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(29548..29631)
                     /gene="idlP"
                     /locus_tag="ACIYSB_003072"
     CDS             complement(29548..29631)
                     /gene="idlP"
                     /locus_tag="ACIYSB_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGC5430066.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(29747..>29887)
                     /locus_tag="ACIYSB_003073"
     CDS             complement(29747..>29887)
                     /locus_tag="ACIYSB_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430067.1"
                     /translation="ADYAEINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIFI
                     KS"
BASE COUNT         7357 a         7472 c         7425 g         7633 t
ORIGIN      
        1 tttatcgtca cttaaacgac gcctttgccg ctcaaccgca aaactgaccg cttacatccc
       61 taaaataacc actcagttat ttaccttact ttacgcgcgc gtaactctgg caacatcact
      121 acaggatagc ggtcaattta cctcctcaaa cgcaacgcaa acctagaacg gcttcggcca
      181 actattaatc aatacatgcc aggttttact atggatacta aaaagatatt caagcacata
      241 ccctgggtga ttctcggaat catcggtgca ttctgcctcg cggtagttgc attacgtcgg
      301 ggggagcacg tcagcgccct gtggatcgtg gtcgcctctg tgtcggtgta tctggtggcg
      361 tatcgctact acagtctgta catcgcccag aaggtgatga aactcgaccc cacgcgcgcg
      421 acgcctgcgg ttattaacaa cgacggtctg aactacgttc cgaccaaccg ttacgtgttg
      481 tttggtcacc acttcgccgc tatcgccggt gctggtccgc tggtgggtcc ggttctcgcc
      541 gcgcagatgg gctacctgcc tggcacgctg tggctgctgg cgggggtcgt gctggccggt
      601 gcggttcagg actttatggt gctgtttatc tcctctcgcc gtaatggcgc atctcttggt
      661 gagatgatca aagaagagat gggaccagta ccggggacta tcgcgctgtt tggctgtttc
      721 ttaatcatga tcatcatcct cgccgtcctg gcgctgattg tggttaaagc cctggccgaa
      781 agtccgtggg gtgtcttcac cgtttgctca accgtaccga ttgcgctgtt tatgggtatc
      841 tacatgcgct ttatccgtcc ggggcgtgtg ggtgaagtct ctgtcattgg tatcgtgctg
      901 ctggttgcct ctatctactt cggtggcgtg attgctcacg atccgtactg gggtccggca
      961 ctgaccttta aagacaccac cattaccttc gcgctgattg gctatgcgtt tgtttccgca
     1021 ctgctgccgg tgtggctgat cctcgcaccg cgcgactatc tggcaacctt cctgaaaatc
     1081 ggcgttatcg tcggcctggc gctgggtatc gtggtgctga acccggaact gaaaatgcct
     1141 gcgatgaccc agtacattga cggtactggc ccgctgtgga aaggcgctct gttcccgttc
     1201 ctgttcatca ccatcgcctg tggtgcggta tctggcttcc acgcgctgat ctcttccggt
     1261 acgacgccga aactgctggc taacgaaacc gacgcgcgtt tcattggcta cggcgcaatg
     1321 ctgatggagt ccttcgtggc gattatggcg ctggttgctg cgtccatcat cgaaccgggt
     1381 ctttacttcg cgatgaatac cccgcctgcg ggccttggca tcaccatgcc taacctgcat
     1441 gaaatgggtg gcgagaacgc gccgatcatc atggcgcagc tgaaagacgt taccgcacac
     1501 gcggcggcga ccgtcagctc ttggggcttc gtgatttcgc cagagcagat cctgcaaacc
     1561 gcgaaagaca ttggtgagcc ttctgtcctg aaccgtgcag gtggtgcgcc gacgctggcg
     1621 gtaggtatcg ctcatgtgtt ccacaaagtg ctgccgatgg ctgacatggg cttctggtat
     1681 cacttcggta ttctgttcga agcactgttc atcctgaccg cgctggatgc gggtacccgt
     1741 tctggccgct ttatgctgca agacctgctg ggtaacttca tcccgttcct gaaaaaaacc
     1801 gactctctgg ttgccggtat cattggtact gcgggctgtg tgggtctgtg gggctacctg
     1861 ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga gcctgtggcc gctgttcggt
     1921 atttccaacc agatgctggc agccgtagcg ctggtactgg gcaccgttgt gctgattaag
     1981 atgaagcgca cccaatacat ctgggtaact gttgttccgg ctgtatggct gcttatctgc
     2041 accacctggg cgctgggcct gaaactgttc agcaccaacc cgcagatgga aggcttcttc
     2101 tacatggcaa gccagtacaa agagaagatt gctaacggta ctgacctgac ggcgcagcag
     2161 attgccaaca tgaaccacat cgttgtgaac aactacacca acgcaggtct gagtattctg
     2221 ttcctgattg tggtgtacag catcatcttc tacggtttca aaacctggct tgcggtgcgt
     2281 aacagcgaca aacgtactga caaagaaaca ccgtacgttc caatcccgga aggcggcgtg
     2341 aagatctctt cgcatcacta accgtgttta gccccgcttc ggcggggctt tgttcttatc
     2401 agagtgaact atgtttggta acttaggaca ggcaaaaaaa tatctcggcc aggcggcgaa
     2461 gatgttgatt ggcattccag actacgacaa ctacgtcgag catatgaaga ccaaccatcc
     2521 cgacaagccg tacatgagct atgaagaatt cttccgcgaa cgccagaacg cgcgctacgg
     2581 cggcgatggt aaaggcggta tgcgctgttg ttaatggaga gagcatgaac ccgattgcag
     2641 ttaccctact caccggtttt ttaggcgcgg gcaaaaccac cctgctgcgc catattctta
     2701 acgaacaaca cggctacaag attgccgtga ttgaaaacga attcggcgaa gtctccgttg
     2761 atgatcaact gattggcgac cgcgccacgc agatcaaaac gctgaccaac ggttgcatct
     2821 gctgttcgcg ctccaacgag ctggaggacg cgctactcga cctgctggac aatctcgaca
     2881 agggcaatat ccagttcgac cgtctggtca ttgaatgcac cggcatggcc gatcccggcc
     2941 cgattattca gacctttttc tcccatgaga ttttatgcca gcgctacctt ctggacggcg
     3001 tgattgcgct ggtggatgcg gtacatgccg atgagcagat gaaccagttc accatcgccc
     3061 agtcccaggt tggctacgcc gaccgtattc tgctgacgaa aaccgacgtc gcaggcgaag
     3121 cagagaaact gcgtgaacgc ctggcgcgca tcaacgcccg cgcaccggtc tacaccgtta
     3181 cccacggcga catcgacctg ggcctgttgt tcaacaccaa cggttttatg ctggaagaaa
     3241 acgtcgtcag caccaaaccg cgtttccact ttatcgcgga taaacaaaac gatatttcgt
     3301 cgattgtggt ggaactggat tacccggtag atatcagcga agtttcccgc gtgatggaaa
     3361 acctgctgct ggaatcggcg gataaactgc tgcgttacaa agggatgctg tggattgacg
     3421 gcgaacctaa ccgcctgctg ttccagggcg ttcagcgcct ctacagcgcc gactgggaca
     3481 ggccgtgggg cgatgaaaaa ccgcatagca cgatggtgtt tatcgggatt cagttgccgg
     3541 aggaagagat tcgggcagcg tttgcggggt tgaggaagta attttggatg atggccgcca
     3601 tgtggcggcc tgaattattt tttgttctga tcgcgcagca tcgcttcacg taaaattgcg
     3661 ttcaggcgag tttgatagcc tttaccaggt cgcttcaacc attccatcac atcagcatca
     3721 atacgcaccg aagcctgagt tttcaatggc cgataaaact taccgcgttc tgcgttagac
     3781 cattgctcat cgctggaagg tgggatatca ctgtaatcaa tatcttcgtc ggatttattc
     3841 gccagcgcct tcagttcggc ctcatgctgc acatcgagtg tagaggaggt ccctcgttta
     3901 tgtttaacca tgctcatagc gattccgttc gttgtgaatc atcaaacact aacaccgcat
     3961 cttcaaagcg gatgccatgt tttacccgat tacttttggc cttgtttgca tcccattcaa
     4021 actccattgg cataattgcc atccctgatt gtatatacaa aaatgtatct acaattattc
     4081 tgcaattata gcgttgatgt tggaaatagc atcacgtttt ggataatcaa ccatgaaatt
     4141 gacaatgagt aagttggact tgattcatcc aatagaattt ctcgcctgac atggcacact
     4201 aaacacctcg ctgtcgttaa gggattaccg tgaaaaacta taaagttatt accccgcttt
     4261 ttcctaccta tgcacaggtt aaagctatga tgaaagccgt ctcaggctat tccctgaaag
     4321 cggtacgcaa tatgattacc gccattcacg agcaaaccgg tacgccgcaa aaacccgttg
     4381 actggtcgga acctgacctg tggatcagcg aacgcttaac gggggaagat gccgacatcg
     4441 cccgtcgtat ttgggatacc gataatcaca tattgaaccc gcgccacagc tacggctgct
     4501 acttattcct caactatccc cagtttgagc tgatggaaag cacgccagat gatacctggc
     4561 agcccacctc gcgcgggcaa aaattcctgc aagatgacga aaaaacgctg cgttcactgg
     4621 acgaccagga aggaattctg caactgcttg agctgctggc tggccgggaa atgtcgcgcc
     4681 gcgccgattt actgccggaa tggcaggcct ttttgcatca gcattcgaaa ttcgcgtctg
     4741 caagttcagt gaaaagcacg ctctactccc gtctctataa ccttatcgac cgtgacatgg
     4801 ttaaccgtga aggcatgagc tatcgcatca ccgataccgg gcgcgcctgg cttggaaaag
     4861 cgttgcctgc acgaaaaagc gatccgcgta aagagctgct ggaagccgtg aagcgctaca
     4921 acgagcagca aaaagagctg ctgcgcgaac aaatcagcac gatgaacccg tacaaatttg
     4981 aacagctggt cggtcaatta ctggaagcaa tgggctacga gcaggtcgaa gtgaccaaag
     5041 cctctggcga taaaggcgtg gacgtgattg gtcaggtgca ggtcggcatc accaccataa
     5101 ccgaggtggt gcaggtcaaa agaatgcaga acaccatcac ccgcccatat atcgaccagt
     5161 tgcgcggggc attgccttac cacaaagcca ttcgcggtac actcatcacc accgggaaat
     5221 ttgcggcgaa atgtgcagaa gcggcgctct ttccgggtgc cgcgcccatt acgcttatcg
     5281 acggcgatcg tttactggaa ctgttaatcg aaaataacgt cggcattcgc cgcagcaatg
     5341 cagtggaatt gctggatgta gatttgcagt tgtttgatga gctggagatg gattaaaggg
     5401 cgaaatccgc cgcctcaacc attacaatag cgacccaatt tatctgaaca gagttcatca
     5461 tggccaaagc accaactaaa aaagccaaag caaagaaagg ctttgaagaa acattatggg
     5521 ataccgcaaa ccagcttcgc ggtagcgttg agtcctccga atacaagcac gtggtgttga
     5581 gcctcgtatt cctgaaattc atcagcgata agtttgaagc acgccgcaaa aaaatgattg
     5641 ccgatgggca agcagacttc cttgagatgg aagtgttcta ccagcaggac aacattttct
     5701 acctgccgga agaggcgcgc tggtcgttta tcaaacaaaa tgccaaacag gacgatattg
     5761 cggtccgaat tgacaccgcc ctctcgacca ttgagaaacg taacccaacc ctgaaaggcg
     5821 ctctgccaga caactacttc agccgtcaga atctggaaac caaaaaactg gcgtcactga
     5881 ttgataccat cgacaacatc gaaacgctgg cacacgaaac tgacgttgaa acgttatcga
     5941 aagaagacct ggtcggacgc gtttacgaat acttcctcgg taagtttgcc gcaactgaag
     6001 gcaaaggcgg gggtgagttc tacacgccca aatgtgtggt tacgctgtta actgaaatgc
     6061 tcgaaccctt tcagggcaaa atttatgacc cgtgctgcgg ctcggcaggg atgttcgtgc
     6121 agtcggtgaa gtttgtcgaa agccatcagg gtaaaagccg tgatatcgcg ctgtatggtc
     6181 aggaactgac agccacgacg tataaactgg caaaaatgaa cctcgccatt cgcggtcttt
     6241 cagctaacct cggcgaacga ccggcagaca ccttctttag cgaccagcac ccggacctga
     6301 aagccgacta cattctggcg aacccgccct tcaacctgaa agactggcgt aatgaagccg
     6361 aattaaccaa agacccacgt tttgctgggt atcgtatgcc gccaaccggt aacgccaact
     6421 acggctggat tttgcatatg ctctccaagc tgtcggctaa cggcacagca ggttttgtgc
     6481 tggcaaacgg ttcgatgagt tctaacacca gcggtgaagg cgagatccgt gcacagatga
     6541 tcgaaaatga tctgattgac tgcatgatcg ccctacctgg acagttgttc tacaccacgc
     6601 agatcccggt gtgtttatgg tttatgacca aatcgaaggc tgccgacccg gccaaaggtt
     6661 atcgtgatcg tcagggcgag acgctgttta ttgatgcgcg taacctcggc accatgatta
     6721 gccgcacaac taaagagtta acgacggaag atattgccac aatcgccgat acctaccatg
     6781 cctggcgcag tacgccagaa gaactggctg cacggattgc gcgtggtgac agcaagttgg
     6841 aaaaatatga agaccaggca ggcttctgca aagttgcgac cctgcaagat attaaagata
     6901 acgactacgt tctgacaccg ggccgctatg tgggtgcagc cgaacaggaa gaagacggcg
     6961 tggcatttga gaccaaaatg cgtgaattgt cgaagacgtt gtttgagcag atgaagcagg
     7021 cggaagaact ggatcgtgcg attcgccaga atctggaggc gctgggttat ggggagtaag
     7081 tggaaacaaa caaccttagg tgatctggcg ctgggctttg atggtgcggt agatgggcct
     7141 tttggttcaa atttaccggc tgcagcttat acagacagtg ggtatccaat tattcgagga
     7201 agtaacttat ctatcggatt agatgatttc aaagataatg attttgtatt tgtaccggag
     7261 cacatatttc aaaaactttc acgtagcgag tgtagggctg gcgacattat attcacaaaa
     7321 aaagggacgt tggggcaaac tgggcttatt agtactaaac atcgtttcga aaaatatttg
     7381 ctatcatcaa atcaaatgcg tcttcgcgta gatcctgaaa aggcattgcc cgaatatgtt
     7441 tattattgtg tttcctcaca aaaagcaatt aacaaaataa ttcaagattc agagtgtaca
     7501 ggcgtcccta aaataaatct cgcttacttg aaaagcttcc ctatttcatt acctagttta
     7561 agcgaacaac aagatataat tgatatttta gggagcctta gtacaaaaat cacgctcaat
     7621 cgccaaatca accaaaccct agaacaaatg tcccaaacct tgttcaaatc ctggtttgtg
     7681 gattttgatc cggtgattga taacgctctg gatgcaggaa atctaatccc ggaagctctg
     7741 caatctcgcg ccgaattgcg tcaaaaagta cgtaatagta cagatttcaa accacttccg
     7801 gcggaaatcc gctcgctttt cccaagtgaa tttgaagaga cggagttggg ttgggtgccg
     7861 aaaggatggt gtattaaaga cttttccgtc attgcgaaat taataaaaga caatgttaag
     7921 tcgaaaaaca tttgtgatga cattcattac attgggttag agcatttgga acgcaagcat
     7981 atttttatca ccaattatgg gaatggtggt gatgtatcca gtaataaatc agctttcgat
     8041 aaaaacgatt tattattcgg aaagttacgc ccttattttc acaaagttgc actagctcca
     8101 ttttcaggaa tatgctcaac tgacatattg gttttccgtg ctaaagagaa catctataga
     8161 tcgttaatgg caatgtatgc ttttacagac gattttgtag catatgcaaa tttgcgttct
     8221 acaggcacaa gaatgcctcg agcggaagct aaagatctgc ttaactataa aattgtttta
     8281 cctaataaaa atgttttaga aaaatttgaa gagctattag aaaattattg gataaaaggg
     8341 caacttaata attacgaaaa tgaaaagtta accgccctcc gcgacaccct gctccctaaa
     8401 ctcatctccg gcgagctatc cgtggaagat ctccccgatc tcatcaaaca aaccgaagcc
     8461 gcataacgca ttttgcccct gaaaaatcag gggctttctg gtaaggtttt ctggggttac
     8521 aggaatcctt accagaaatt agccagggtt ggagcgcgat atgagtctct ctttcagtga
     8581 agcaaaatta gaacaagcga tcattgaact gctgcaggat cagggatatc aacacctgat
     8641 cggcgataat gtcccacgtt caagtctcga tcaggtcatc atcgaagacg atctccgtca
     8701 ttatttagcg gcacgctacc agcctgatgg cattactgaa gaagagattc aacggctgat
     8761 caaacagttc accacgcttc cggcctccga cctttatgaa agcaacaaaa cattttgtcg
     8821 ctggctggca aatggttttc tgttcaaacg cgacgatcgg caacaaaaag atctctatat
     8881 tgaattgctc gacacccggc atctacccgc cgcactgtgc cagatatttg acgccgaaga
     8941 tgtcccgttg caacaggcgg cagaactccc gccctcctat attaatccgc cgcttaaccg
     9001 gattaagatt gttaaccagc ttaaaatctc cggcaaagat aatcagactc gtattcctga
     9061 cggcattctc tatatcaacg gtctgccact ggttgtcttt gaatttaaaa gtgcggtacg
     9121 cgagcaggaa gccagtattg gcgatgcctg gaagcagctc tgtaaacgct accgccggga
     9181 tattccacaa ctgtttatct acaacgcgct ctgcattatt agcgatggcg taaataaccg
     9241 gatgggcaac ctgtttgcgc cctatgaata cttttactca tggcgcaaag tcaccggtaa
     9301 tgaaaaccgt gaacaggatg gaattccatc actgcactca atgattcagg ggctgtttca
     9361 tccggtacgt ctgctggatg tgattaaaaa ctttatctgc ttcccggata aagccaagca
     9421 cgaagtaaaa atttgctgcc gatatccgca gtactatgcc gcccgcaaac tctattacag
     9481 catcaagcaa gcgcgtaaac ctttcggtag cggtaaaggc ggcacctact ttggcgcaac
     9541 gggctgtggc aaaagttaca ccatgcaatt tttaacgcgt cttttgatga agagcgtgga
     9601 gtttgccagc ccgaccattg ttttgatcac cgaccgcacc gatctggacg atcagctttc
     9661 tgcgcaaatg tgcaacgcca aaaattacat tggcgacgac accatcgttc ccgttaccag
     9721 ccgtgaagat ttgcgtaatc aactggcggg acgcaatagt ggcggagtct ttctgacaac
     9781 gatccataaa ttcaccgaag acaccgaact cctttctgaa cgcaacaata tcatttgcat
     9841 ctcggacgaa gcacatcgta gccaggttaa cctcgaccag aaagtcatcg tcgataaaga
     9901 tagcggaaaa gtgcgcaaaa cgtatggctt tgcgaaatac ctgcacgatt cactgccaaa
     9961 cgccacctat gttggcttta ccggcacacc gattgacgcg acgctcgatg tcttcggtga
    10021 ggtgatcgac agttacacca tgaccgaatc cgttcaggat gaaatcactg tacgcatcgt
    10081 gtacgaaggc cgtgcggcta aagtgatcct ggactccagc aaactggaag aagtcgaaaa
    10141 gtattacgaa gagtgcgcaa acgcaggcac caatgagtgg caaatcgacg aaagcaaaaa
    10201 agccaccgca accatgaatg cggtactggg tgatgaagat cggttgaaag ccctcgcgga
    10261 agattttgcc aaacattatg aaaaacgcgt agccgaaggt tccaccgtaa aaggcaaagc
    10321 catgtttgtt tgtgccagcc gtgaaattgc ctgggatttc taccgtcagc ttaaagctat
    10381 tcgccctgtc tggtttgaag tgaagcaagc acccgatggc gtcgtcctga cggaacagga
    10441 acaaaaagag ttaccgcctt ctgaaatggt gaagatggtc atgacgcgcg gtaaagatga
    10501 cgacgaggcg ctttatgatt tactgggcac aaaagaatat cgtaaagagc tggataagca
    10561 gttcaaaaac gctaaatcga atttcaaaat tgccattgtt gttgatatgt ggctgactgg
    10621 tttcgacgtc ccggaactgg ataccatcta tattgataaa cccttacaaa aacataatct
    10681 tatccagacc atttctcgcg ttaaccgtaa actggaaggc aaaagtaaag ggttagtggt
    10741 ggactacatc ggcattaaaa gtcagatgaa ccaggcactg gcaatgtatt cccgcattga
    10801 tgccaccaac tttgaagata ttcagcaatc ggtaatagaa gttaaaaacc atctcgattt
    10861 gttggggcaa gtattttatg aattcgacag tcgggattat tttagtggtg agccacaagc
    10921 gcaattatcc tgcctcaacc gcgcggcgga atttgttctg cgcactcaga aagttgaacg
    10981 tcgttttatg ggactggtta aacgcatgaa agccgcctac gacgtctgct gcggcagtga
    11041 agcactatca cagacagaac gtgatcatat tcactattat cttgctgttc gttcaattgt
    11101 tttcaaactg acgaaaggtg acgcaccgga tgtcacccag atgaatgcac gcgttcgtga
    11161 aatgattgca gaagcgctaa aagctgatgg cgtagaagaa atttattttc ttggcgataa
    11221 aaaagcggaa tccatcgata tttttgacga agattatctg gcgcgaatta acaagatcaa
    11281 acttccggca acaaagatcc agttattaca aaaattactg gaaaaagcga tcagcgactt
    11341 caagaaagtg aaccagttgc aagggattaa cttcacccgc cgcttccagg cgattataga
    11401 tcgttataat gagcggcgag aagatgatgt actcaacggt gaagaattcg atacattcag
    11461 tcaggaaatg accgatatta tctatgatat taaaaccgaa atgggcacct gggccgattt
    11521 aggtattgat attgaagaaa aagccttttt cgacattctt gctcatatgc gcgataaata
    11581 tcagttcacc tatgacgatg aaaaaatgct gtcactggca aaagagatga aaagcgtggt
    11641 tgacaacaca tcgaaatatc ctgactggag taaacgcgat gatattaaag cgaaactgaa
    11701 agttgaactt attcttcttc tacacaagca taagttcccg ccagtagcga atgatgatgt
    11761 ttatatgggt gtgctggcac aggcagagaa ttttaaagaa catcatatga gtgctttaaa
    11821 ttaattatga aggccggata cactttgtta tccggccatt ctgaattaac attaatttct
    11881 cttccagatc tttccagtaa cgtttctatt attttctatt tttagctgga ctttccctgt
    11941 atttactgat gatatatcca ggtatttagc gcggtgcgga tgtgcgccaa cacacccgca
    12001 ccgctaatca caatcactat tatcggagaa tagcagttat gactgacacg cattctattg
    12061 cacaaccgtt cgaagcagaa gtctccccgg caaataaccg tcaattaacc gtgagttatg
    12121 cgagtcgcta tccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg
    12181 aagccgccgg ttttgctacc ggcacggcgg tagatgtcaa agtgatgaaa ggctgtattg
    12241 tcctcaccgc ccaaccaccc gccgccgaag agagcgagct gatgcagtcg ctgcgccagg
    12301 tgtgcaagct gtcggcgcgt aaacaaaagc aggtgcggga atttattggg gtgattgcgg
    12361 gtaaacagaa agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc
    12421 gccgcgttat accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat
    12481 aatgtaagga agttgtgcga ggtcactgaa accaatttcc acatcgccat tgccccatcg
    12541 cccaacattt gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat
    12601 attaagcgac aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttcagc
    12661 tttaaacgca atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac
    12721 ttcatcgcga agatgatcga ataacacccg gctatgcgaa ccatcagcaa gaaaaggata
    12781 atcactcagg ctgtaactgg ttttgttctc cggctgcgcc cgataaacag ctaaaacctc
    12841 ttcaggaaga gcaggcgctt gccagacctg aaccgcgaga tcggccagac gatcggcacg
    12901 ggcgcgaatg gcggcttcat cccatttttc gcactgtcca agaccgatat tcaaataaag
    12961 cgggctatgc ttaaagccgt cttcaatatc gcgtttttcc gcgaaaggtc tgtcactgta
    13021 gcgggagtta tagcgcgtta aagtgagatt ccccaacgta tgcaacagtt ctttatgcac
    13081 acgctgccag tcgcttccca gctcttcacg ccatttagcc gacaaatttt cgttctgcgg
    13141 cataatatgc tcaatcgtaa actcttcctc gcgctctctg cgtttatcat tttccagtcg
    13201 tcgtaaccag tagctgcgac tgcggaagtt atagagatcg cgaactttta attcccgttt
    13261 aaattcatca tcgttgggaa aacgacgata tgaaggcagg ttcagaaaat gcacctgaat
    13321 actttccaga tatttttctt tattaatgac tttataaaaa gtggcaaatg tcttgttcaa
    13381 agaattcgtc ggaatcgcac atactgcacg acggaaaaca taagattcaa ttaaacgaat
    13441 tatgctcagg aaatcttcgt gaggcaaatc gccattttta tagtcatgat aaagcgccag
    13501 taagaaagga tatgccacat caacctttaa ctcgcgcaaa tcctgaaaag ccgtagcaag
    13561 cgatttgtca ctttcttttc ccaatgccat tgcacagtaa tactccgcgt agatgtgaat
    13621 atcttcaacc agccgatcta cgcctttttc agcaacactc tggctgcggg catggagttt
    13681 aaatgcctca tagacttcat ctgtccgggg gatctccccc gtttttaccg tcagataatg
    13741 acgcataaaa ctgtcaaaat attcgctgta accttgctga ccaaaagcga cttccattgg
    13801 acgccagtga tcttcataca accgggtttg atgctctggt tcgaggccca tcagaataaa
    13861 attgcgcacc agatcggcct gacttaacgc cttaccggtg gagttcatac tttcaaaaat
    13921 cagttgcgga ttatcctgac cacgattaag cgccacatcg acaattaata attttgctaa
    13981 cccacgacat aaggggatca agtcatcacc gagtttggca atttgttcat caaagaaagt
    14041 aaagttttcc attattcgat gggaatagtt ttctggcatt ggtctttgtt ttattaacgc
    14101 cagcaaactg tctttatcag tctcggtcag cagtaattta aatcctttct cgccggactc
    14161 ataaggattg agcaaataat aattacgcaa tttcattgcc gaaaagccat caaaaacttc
    14221 gtcttcgcca agatggcgcg ataaagcctc agtcagcaac attgcggtcg tcagccgctg
    14281 ctgaccatca atgaccagca acggagaaat accagaaacc tgatacaatc cctgctctat
    14341 ataaacaacc gaaccgataa aatgcgctga tatatcgtca cgctttccgg cacgaatgat
    14401 gtcgtcccaa agttgccgac attgttgttc tgtccatgaa taagtccgtt gataaatggg
    14461 aataacaaac tgctgcgaac gttttaaaaa atcgagtagg cgagcttctg tagctttcat
    14521 tctgacgtct ttccgtgaag cgatgatttc gcaataataa ccacttcctt gtggaggtta
    14581 tagagaaatc ctgtttatgc gcgacgaaca gcccccatca ctccacatcc tccctgcgta
    14641 acccaatatc cctcaaccgc tcatcactca tctgctgtaa cacccgtcgg gtctgcattt
    14701 gccgccgcca acgcctcacc gcctgccaga gttgcacgaa gccgataaac ggcgcgttgg
    14761 ctttgttttc gtgaaattcc atctgctgcc tcctcatctt tcagaggctt catcatccgt
    14821 cttctggcag ttcacaatac agatgcaaat attagtttta tttaacatac agacaggtta
    14881 aaacagcatc tgcatccggt tttttagcct catctgtacc gttttttctc tctgtatggt
    14941 ccaaataagg aatacagcat gacgcgttat caacatctgg cgactctgct tgccgagcgg
    15001 attgagcaag ggctgtatcg tcacggggag aaattgccgt cggtgcgcag cttaagtcag
    15061 gagcacggcg tcagtattag caccgtgcag caggcgtacc agacgctgga gacgatgaag
    15121 ctcatcactc cgcagccgcg ttcgggttat tttgtcgcac aacgtaaagc ccagccgcca
    15181 gtaccgccga tgacgcgtcc ggtgcagcgc ccggtggaaa ttacccagtg ggatcaggtg
    15241 ctggatatgc tggaggcgca tagcgacagt tccattgttc cgttaagtaa aagcacgccg
    15301 gatgtcgaaa cgcccagcct gaaaccgctg tggcgggagc taagccgggt ggtgcagcat
    15361 aatctgcaaa ccgttctcgg ttatgacttg ttagccggtc agcgagtgtt gcgagagcag
    15421 attgcccgcc tgatgctcga cagcggctcg gtggtcaccg ccgatgacat catcattacc
    15481 agcggctgcc ataactcgat gtcgctggcg ttaatggcgg tgtgtaaacc gggcgatatt
    15541 gtcgcggtcg aatccccctg ttattacggt tcgatgcaga tgctgcgtgg catgggcgtg
    15601 aaagtgattg aaatcccaac cgatccagaa acaggcatca gcgttgaagc gctggaactg
    15661 gcgctggaac agtggccgat taaaggcatc attctggtgc caaactgtaa taatccgctg
    15721 ggatttatta tgccggacgc gcgcaaacgg gccgttctct ctctcgctca gcgtcatgat
    15781 attgtgattt ttgaagatga tgtctacggc gaactggcaa cggagtatcc gcgcccgcgg
    15841 accattcatt catgggatat cgacgggcga gtgctgttgt gcagctcgtt cagtaaaagt
    15901 attgcacctg gcctgcgcgt gggctgggtc gcgccgggac gttatcacga taaactgatg
    15961 catatgaaat acgccatcag cagctttaat gtgccgtcca cgcaaatggc ggcggcaacg
    16021 tttgtgctgg aaggtcacta tcatcgccat atccggcgga tgcggcagat ctatcagcgc
    16081 aatctggcgc tttatacctg ctggatacgg gaatattttc cctgcgaaat ctgtattacg
    16141 cgcccgaaag gcggattttt actgtggata gaattgcctg aacaggtcga tatggtctgc
    16201 gtcgcgcggc agctgtaccg catgaaaatc caggtggcgg caggctcaat tttctcagct
    16261 tccggcaaat accgtaattg tctgcgcatc aactgcgctt tgccgctcag cgaaacctat
    16321 cgcgaagcac taaaacaaat tggcgaggcc gtgtatcggg caatgaaatg aattcgaaat
    16381 agtggcgagc cccctgattt cctctcacga atagcgtgtg actcgtttta tctttgcagg
    16441 atcatctcca cgttccagca tggcttaggc aaaatgcgct ttgttcatgc cggatgccat
    16501 gtgaacgcct taccaagtct acaaaatctt taaagttcaa tatagtgaat gggttatgta
    16561 gacctgataa gcgtagcaca tcaggctgct ttgcgtttgt catcagtctc tgattagtac
    16621 tcgatccatg gatcgcgctt gctttcaggc aagggttgct ctaatgcctg catttgctct
    16681 gccgaaagtc cggtaatctt ttgtatccat tcaggatctg ccccattctg caacaacaat
    16741 cggaggaggc gctgttcccc ttcttctttc cctttttcca tccccaacag ccatccatca
    16801 ttataaattc gctctgcaat cgtcattatt cgctccctgt gttgtggcat tcgacgggta
    16861 agttcgctga taaacttctt aaaacgcgct tcatcgccag tcagtaaaat gtaatttaac
    16921 agcgcggtta tctggctgtc attagcacac tctgtaacca gtaatagtac cagttgatcg
    16981 ataagcccca tcagatcgcg ctggcgaata tgcttttgga tcaactccaa cagggcgact
    17041 ctgcgatgct gcacaatctc gtcgtctggc acgacagtga catccaccag cgggaacgct
    17101 gcggtataaa gcttccgtgc ggtggtcggg tcagcaaatt cgtccagcca gcacagggac
    17161 cagggataag gactacggct accgtgataa aacagcatcg ggatgaccag cggtagcggc
    17221 cggcgtttat catgctcgat atggcgctgc atcaccgcca tggaatatcg catcaggcga
    17281 aaggccatat ggatatcctc gcggctctga tgttcaatca ccacataaat atagccatcg
    17341 ccttcgcggg tctttaccga ccacagaata tcggaatgta gcgcccgcaa tttttcatcg
    17401 acaaagctgg cggattccag ttttaagctg tcgagatcgc acagttcacg taaatctttg
    17461 ggtaagtgga tctccataaa atcccgcgcg gtgtcagggt gcgtgagaaa ggatttaaat
    17521 aatgcgtcat gcggcgtgct ggttgtgaag tttgtcatgg cgatccgtca cctgtagata
    17581 agatggcccg actgtagcga ctaacaaaac cattctcacc cggcaatttc ccattccccg
    17641 aggtgctttc caggctattt ttgttcctct gcaaacgata catttttccg gaaacaggct
    17701 cgcgttttac atttcctgta cccggcagat attccccttt ccaccgcact cttttgatta
    17761 accgcaaatt ttaattacac ttaaggtgta tattttctat gcaacccatc aattcaagag
    17821 gtgtaatgtg ctgatgacta tttgtaatcg ttatacattc tgacctggag tcagaaggta
    17881 tttctctgtc tgtgtgttca caggcagtgt ggttgattac atgaaattca gtacatttgt
    17941 ccttctcgcc tgcctttcgt cattaccgac ggtattgaat ttcgttttcc ccattggggt
    18001 tctccggaca aggagttgtt tgttatgcca cgtttttttg cccgccatgc cgccacgctg
    18061 tttttcccga tggcgttgat tttgtatgac tttgctgcgt atctgtcgac ggacctgatc
    18121 cagccaggga tcattaatgt ggtccgtgat tttaatgccg atgtcagtct ggcccctgct
    18181 gccgtcagtc tctatcttgc tggcggtatg gcgttacagt ggctgctggg gccgctttcc
    18241 gacagaattg gccgcaggcc ggtgctgatt accggggcgt taatttttac ccttgcctgc
    18301 gccgcgacaa tgttcaccac gtcaatgaca caatttctta tcgcgcgtgc aattcagggc
    18361 accagtatct gttttattgc caccgttggt tatgtcacgg tgcaggaggc gttcggacag
    18421 acaaaaggga tcaagttgat ggcgattatc acctccatcg tactgattgc gccgattatc
    18481 ggcccgcttt ccggcgcagc tctgatgcac tttgtgcact ggaaagtcct ttttgccatc
    18541 attgcggtta tgggttttat ctcatttgtt ggcttactgc tggcgatgcc agagacggtg
    18601 aagcgcggcg cggttccatt tagcgccaaa agcgtcttgc gcgattttcg taatgtcttt
    18661 tgcaatcggc tgttcctctt tggcgcagca accatctctt taagctatat cccgatgatg
    18721 agttgggtgg ctgtctcgcc ggtgatcctg atcgacgcgg gcggcttaac aacttcgcag
    18781 ttcgcctgga cacaggttcc ggtgttcggc gcggtgattg tcgcgaatgc catcgtggcg
    18841 cgtttcgtta aagatccgac cgaaccgcgc tttatctggc gtgccgtacc ccttcaactg
    18901 gttggcctcg cgctgttgat tatcggcaat ctgctgtcac cgcacgtctg gctgtggtcg
    18961 gtgctgggca ccagtctgta tgctttcggg attggtttga tcttcccgac cttattccgc
    19021 tttacgctgt tttccaataa cttaccgaaa gggaccgtct ccgcatcgct aaatatggtg
    19081 atcctgatgg tgatgtcggt ctcggtcgaa atcggccgct ggctgtggtt taacggcggt
    19141 cgcttgccgt ttcatctatt agccgttgtg gcgggcgtta tcgtcgtttt caccctggcg
    19201 ggattgctca atcgcgtgcg ccaacatcag gctgccgagc tggcggagga gcggtgattt
    19261 ttgcgcgatc ctaccgtcag gctctattct taacgttatg aataaactca ttgaactcag
    19321 acgcgccaaa atgttggcgc tctctttatt gcttatcgcc gctgctacct ttgtcgttac
    19381 gctgtttttg ccgcccaatt tttgggtgag cggcgtgaag gcgattgctg aagcggcgat
    19441 ggtcggcgcg ctggcggact ggtttgcggt ggtggcgctg tttcgccgcg tgccgattcc
    19501 gatcatttct cgtcatacgg cgattatccc gcgtaataaa gaccggattg gcgaaaatct
    19561 cggtcagttc gtgcaggaaa aatttctcga tacccaatcg ctggtggcat tgattcgacg
    19621 tcacgaaccg gcgttgctga ttggtaactg gtttagccag ccagaaaacg cccgccgcgt
    19681 tggtcagcat ctgttgcaga tcatgagtgg ttttcttgaa ctgaccgatg atgcgcgtat
    19741 tcagcgcctg cttaagcggg cggtacaccg agcgatcgat aaggtcgatc tctccggcac
    19801 cagtgcattg atgctggaga gcatgaccaa aaatgatcgt catcaggtgc tactggatac
    19861 gctgatcgca cagttgatcg ccctgctcca gcgcgataaa tcgcgcaagt ttatcgccca
    19921 gcaaattgtt cgctggctgg agagcgagca tccactgaaa gccaaaattt tgcccactga
    19981 atggctgggc gaacatagcg cggagttggt ttctgacgcg gtgaattctt tgcttgatga
    20041 tatcagccgc gatcgtgcgc atcagatccg ccatgcgttt gatcgcgcca cttttgccct
    20101 gatcgacaag ttgaaaaacg atcctgaaat ggcagcgcga gccgatgccg taaaaagcta
    20161 tctgaaagaa gatgaagctt ttaaccgcta tctcagtgaa ttgtgggggg atttgcggga
    20221 atggctgaaa gcggatatca acagtgaaga ttctcgcgtt aaagaacgta tcgcgcgggc
    20281 gggtcaatgg tttggcgaaa cgttaattgc cgatgatgcc ttgcgggcgt cgttaaatgg
    20341 tcatctggaa caagccgcgc accgcgtcgt gcctgagttt tccgcattcc tgacgcgaca
    20401 tatcagcgat acggtaaaaa gctgggatgc gcgggatatg tcgcggcaaa tcgagttaaa
    20461 tatcggcaaa gatctacagt ttatccgtgt caacggtacg ctggttggcg gttgtattgg
    20521 gctaattttg tatttgcttt cgcagctccc ggccttgttc cccctcggca atttttagaa
    20581 atccatgaaa aactaagtgg ctaaccgcga gagagatcaa ataaaccaca ctaaaagaat
    20641 ggtaataatg tggcctcttt tcattatctc ccgtggtacg gggaaggaaa atcatgtcac
    20701 ttgtcaccga tctacccgcc attttcgatc agttctctga agctcgccag aaaggctttc
    20761 tcaccgtcat ggatctcaag gagcgcggca ttccgctggt tggcacttac tgcaccttta
    20821 tgccgcaaga gatcccgatg gcagccggtg cagttgtggt ttcgctctgt tccacctctg
    20881 atgaaaccat tgaagaagcc gagaaagatc tgccgcgcaa cctctgcccg ctgattaaaa
    20941 gcagctacgg cttcggcaaa accgataaat gcccctactt ctacttttct gatctggtgg
    21001 tcggtgaaac cacctgcgac ggcaaaaaga aaatgtatga atacatggcg gagtttaagc
    21061 ctgttcatgt gatgcagttg ccaaacagcg ttaaggacga tgcctcgcgt gcattgtgga
    21121 aagccgagat gctgcgcttg caaaaaacgg tagaagaacg ttctgggcac gaaattagcg
    21181 aagatgctct gcgcgatgcc attgcgctga aaaaccgcga acgtcgcgca ctggctaatt
    21241 tttatcatct tgggcagtta aatcctccgg cgcttagcgg cagcgacatt ctgaaagtgg
    21301 tttacggcgc aaccttccgg ttcgataaag aggcgttgat caatgaactg gatgcaatga
    21361 ccgcccgcgt tcgtcagcag tgggaagaag gccagcgact ggacccgcgt ccgcgcattt
    21421 taatcaccgg ctgcccgatt ggcggcgcag cagaaaaagt ggtgcgcgcg attgaagaga
    21481 atggcggctg ggttgtcggt tatgaaaact gcaccggggc gaaagcgacc gagcaatgcg
    21541 tggcagaaac gggcgatgtc tacgacgcgc tggcggataa atatctggcg attggctgct
    21601 cctgtgtttc gccgaacgat cagcgcctga aaatgctcag ccagatggtg gaagaatatc
    21661 aggtcgatgg cgtagttgat gtgattttgc aggcgtgcca tacctacgcg gtggaatcgc
    21721 tggcgattaa acgtcatgtg cgtcagcagc acaacattcc ttatatcgct attgaaacag
    21781 actactccac ctcagatgtc gggcagctca gtacccgtgt cgcggccttt attgagatgc
    21841 tgtaaggagt ggcagtgaca tattcgattg gcattgattc tggttctacc gccaccaaag
    21901 ggatcttact ggcagacggc gtgattacgc gccgtttcct cgttccaacc ccctttcgcc
    21961 cggcaacagc aattactgaa gcctgggaaa ctctgcgcga agggttagag acaacgccgt
    22021 ttctgacgct caccggctac gggcggcaac tggtggattt tgccgataaa caggtaacgg
    22081 aaatctcctg tcacgggctg ggcgcacggt ttcttgcgcc tgcaacgcgc gcggtaatcg
    22141 acatcggtgg tcaggacagc aaagtgattc agcttgatga tgacggtaac ctgtgcgatt
    22201 tcctgatgaa tgacaaatgc gcggcaggca ccgggcgttt cctggaggtg atctcgcgca
    22261 cgcttggcac cagcgtcgag caactcgaca gcattaccga aaatgtcacg ccgcacgcca
    22321 tcacgagtat gtgcacagtg tttgctgaat cagaagtgat cagcctgcgc tcagcgggcg
    22381 tcgcgccaga agcgattctc gccggagtga ttaacgcgat ggcgcggagg agtgccaatt
    22441 tcattgctcg tctctcctgt gaagcgccga ttctgtttac tggtggcgtt agtcattgcc
    22501 agacgtttgc ccggatgctg gaatctcacc tgcgaatgtc ggtaaatacc catcctgatg
    22561 cgcaatttgc tggcgcaatt ggcgcggcgg taattggtca acgagtgagg acacgccgat
    22621 gaaagagttt ttatttttat ttcactccac ggtcggcgtc atacaaaccc gcaaggcgtt
    22681 gcaggcagcg ggcatgactt ttcgcgtcag cgatattccc cgtgatttac gcggcggctg
    22741 cgggttatgc atttggctga cctgtccccc cggcgaggaa atacaatggg tgatccctgg
    22801 gctgactgag tcaatttatt gccagcagga tggtgtctgg cgctgcattg cacattacgg
    22861 ggtttctccg cgttaaggat tcattaaggt cgctacgtat acttctcgat atcttattga
    22921 tttgccttaa agggattacc gtgacaaaaa gtatttcgtt aagtaaacgc atctccgtta
    22981 ttgtgattct ttttgccatt gtggcagtgt gtactttctt cgtgcaatct tgcgcccgta
    23041 aaagcaatca tgcagcatca tttcagaatt accacgccac cattgacggc aaagaaattg
    23101 caggcataac gaataatatc tcttcgctga cctggtcggc acaaagcaat actcttttta
    23161 gcactatcaa caaacctgcc gccattgttg aaatgaccac caatggtgat tttatccgca
    23221 caattcctct ggattttgtt aaggatctcg agacgatcga atacattggc gataatcagt
    23281 ttgtcattag cgatgagcgc gattacgcca tttatgtgat ttcactgacc ccaaactcgg
    23341 aagtaaaaat cctcaaaaaa ataaaaatcc cgctgcaaga ctccccaacc aattgcggtt
    23401 ttgaagggtt ggcttattct cgccaggatc acactttctg gttttttaaa gagaaaaatc
    23461 cgattgaggt ctacaaagtt aacgggctgt taagtagcaa tgaattacac atcagcaaag
    23521 acgaggcact acaacggcaa tttactttag atgatgtgtc cggcgcagaa tttaaccagc
    23581 aaaaaaatac gcttctggtg ctgtctcatg aatctcgcgc actccaggaa gtaaccctgg
    23641 ttggcgaggt tatcggtgaa atatcactaa ccaaaggtag tcgaggtttg tcgcataaca
    23701 tcaaacaggc cgaagggatt gcgatggatg cctctggtaa tatttatatc gttagcgaac
    23761 caaatcgttt ttatcgcttt acccctaaat catcgcatta acaggctggc atatttcacc
    23821 tttttgttat taacttgtta acaacaaaaa tgacatacaa ggttaaacat gccttcgtcc
    23881 tcgcatcccg tagaacgctt ttctttcagc accgcgcttt tcgggatgct ggttctgacc
    23941 ttaggtatgg gattaggtcg cttcctttat acgcctatgt tgcccgtcat gatggcggaa
    24001 ggggcgtttt catttagcca gctctcgtgg attgccagcg gcaactatgc ggggtatctg
    24061 gctggcagtc tgctattttc gtttggcgca tttcaccagc cgtcgcgcct gcgcccattt
    24121 ctgttggttt ctgccctggc gagcgggttg ttgatcctcg caatggcatg gttgccgcca
    24181 tttattctgg tgttattgat tcgcttcctg gcgggtgtcg ccagcgccgg tatgctgatt
    24241 tttggttcga cgctgattat gcagcacacg cgccatcctt ttgtgctggc agctttgttt
    24301 tctggcgttg gcattggcat tgcactgggc aacgaatatg ttctggcagg cctgcatttt
    24361 gccttctcct cgcaaacgtt atggcaaggc gccggggcgc tttctggcat gatgctgatt
    24421 gcacttacgc ttttaatgcc ctcgaaaaaa cacgccatcg caccaatgcc attggcaaaa
    24481 acggagcaac agataatgag ctggtggtta ctggctattc tgtacggcct tgcgggattt
    24541 ggttatatca tcgtcgcgac ttacctgccg ctcatggcga aagatgcagg ctcaccattg
    24601 ttaaccgccc atctctggac gttagtcggc ttatcaattg tgccaggctg ctttggctgg
    24661 ctatgggcag caaaacgttg gggggctttg ccttgcctga cggcgaattt gctggtgcag
    24721 gctatcagtg tgctgcttac tctcgccagc gactctcctc tcttgcttat cattagtagt
    24781 atcggttttg gcggtacctt tatgggcacg acatctctgg tgatgactat cgcccgccaa
    24841 ctgagcgttc cgggaaatct caatctttta ggctttgtga cgctcattta cggcattgga
    24901 caaatcctcg gcccggcgct gaccagtatg ctcggcaatg gaacatcggc gcttgccagc
    24961 gccactctct gcggcgcggc ggcgttgttt attgctgcgc ttatcagcac agtacaatta
    25021 tttaagctgc aagtggtcac ttcatgagga gtgtttccct taccactcaa gaaattcgac
    25081 cgcaaccgtt gatgtcagcc agaccccgtt ttccgcttgc cagaaaggga ggcctcgttt
    25141 cgccatttcc tgtgctttga cggttaaaat aaccggcgat ccgtgtcttg ctcccacttt
    25201 gcgtgccgtt gcttcatcag cagagagatg tacgtagtga cgctcacctg caatcagccc
    25261 ctgttttttt atctcgtcaa ggaaacggct tgccgtaccg tggtagagaa actgtggcgg
    25321 tgttttttcg gcaaaggaaa tagcgacctg cgaagtcgaa tgcccctgaa ccgcgcggat
    25381 gcacctacca tcactggaat aactaaaacg ctttttatcg ctggtggcaa ccacggtatc
    25441 cagcaatgca cgggttaacc gcttgcccgc tttttgggca caaagtatga gcttatcgat
    25501 atccgcccat ccttcacggt ccagtacgat accaatcgcc tccggtttgt gtcggaggac
    25561 aaaacttaaa aacttgctcg tatccgccag ctctttttcg ttgtactttg ccatcgcgtt
    25621 ttcgtccctg gattatctgt tttttattta ctatgtcatt gtcactgggt ttatgaattt
    25681 tctgcaactc aatcctgcgt agaacctttt ccacacagcg taattttacc gctatgcaaa
    25741 aaacgcatag tcagaaacat acttgcattt tccccatacc cacatggctg ataaggtttt
    25801 tcagctccag gaattttcct ggctcaagca acacacaaac acaaaaaaac aatggggatt
    25861 gttatgacac aacaagggga tgctgtagcc ggggagctgg cgacagaaaa agtgggcatt
    25921 aaagggtatc tggctttttt tctcaccata atcttttttt ccggtgtttt ttccgggaca
    25981 gacagttggt ggcgcgtttt tgatttttcc gttctgaacg gatcgtttgg gcaactgcct
    26041 ggtgcgaacg gagccaccac ctcgtttcgc ggcgcgggtg gcgcgggggc taaagatggc
    26101 tttctctttg cgctggagct ggcaccgtca gtcattctct ctctggggat tatttcaatt
    26161 accgatggtc ttggcggatt acgcgccgcg cagcagttaa tgacgccagt gctaaaaccg
    26221 ttgttgggca ttccagggat ctgctcgctg gcgctgatcg ctaacctgca aaacaccgat
    26281 gccgccgcag gtatgaccaa agagctggcg caggaaggtg aaattaccga acgcgataaa
    26341 gtgattttcg ccgcatatca aaccagcggt agcgccatta tcaccaacta cttctcttcc
    26401 ggcgtcgcgg tgtttgcctt tctgggcacg tccgtaatcg tacctttagc agtcatcctg
    26461 gtgtttaaat ttgtcggagc caacattttg cgcgtctggc ttaacttcga agaacgtcgc
    26521 aacccaacgc aaggagcgca agcatgacaa ctcaggtccg caaaaacgtc atggatatgt
    26581 ttatcgatgg tgcgcgccgt ggctttacta tcgccacgac caacctgctg ccaaacgtgg
    26641 tgatggcgtt tgtcatcatt caggcgctga aaattaccgg cctgctcgac tgggtggggc
    26701 atatctgtga acccgtaatg gcgctgtggg gattacccgg tgaagcggca accgtccttt
    26761 tagccgcatt aatgagtatg ggtggcgcag tcggtgttgc cgccagtctg gcaaccgctg
    26821 gcgcattaac cggtcacgat gtcaccgttt tactgcccgc gatgtattta atgggcaacc
    26881 cggtgcaaaa tgtcggtcgc tgcctgggga cagccgaagt gaatgccaaa tattacccgc
    26941 atatcattac ggtctgcgtg attaacgcat tgctgtcgat ctgggttatg cagcttattg
    27001 tttaacaagg agttaccatg attgattata ccgcagccgg ttttaccctg ctgcagggag
    27061 cgcatttgta tgcgccggaa gatcggggaa tttgcgatgt cctcgtcgct aacggcaaaa
    27121 ttatcgccgt tgccagtaat atcccttctg acattgtacc ggactgcacg gttgtcgatc
    27181 tcagcgggca gatcctctgc ccaggtttta ttgatcaaca cgtccatttg attggcggtg
    27241 gcggcgaagc aggtcccacg acgcgcacgc cggaagtggc gttaagtcgt ctgacggaag
    27301 caggcgtcac gtcagtggtt ggtctgctgg gcaccgactc tatctctcgc cacccggaat
    27361 ccctgctcgc caagacccgt gcgctcaatg aagaaggcat cagcgcctgg atgctgaccg
    27421 gcgcttatca tgtcccttcc cgcactatta cgggttccgt ggaaaaagac gtggcgatta
    27481 tcgatcgcgt gattggcgtg aaatgcgcca tctctgatca ccgttctgcc gcaccggacg
    27541 tttatcacct ggctaatatg gcggcggaat cccgcgttgg cggtttgctc ggcggtaaac
    27601 ctggcgtcac cgtgttccac atgggcgaca gcaaaaaggc gttacagcct gtctatgacc
    27661 tgctggaaaa ctgcgatgtg ccgattagca agttgctgcc gacccacgtt aaccgcaacg
    27721 ttccgttgtt tgagcaggcg ctggagttcg cgcgcaaagg cggcaccatc gatatcacca
    27781 gcagcattga cgaaccagtc gcccctgccg aaggtattgc ccgcgccgtt caggcgggta
    27841 ttccgctggc acgcgtcacc ctcagctccg acggcaacgg tagccagccg ttcttcgatg
    27901 acgaagggaa tttaacccat atcggtgttg ccggttttga aacgttgctg gaaaccgtgc
    27961 aggtgctggt caaagattat gatttcagta tcagcgatgc cctgcgcccg ctcaccagta
    28021 gcgtagccgg tttccttaac ctgagcggga aaggcgaaat tctgccaggc aatgacgcag
    28081 acttactggt catgacgcca gaattacgca ttgagcaggt atacgctcgc ggcaagctga
    28141 tggtcaaaga cggcaaagcc tgcgtgaaag gaacgtttga aacggcataa acggttgtca
    28201 gccgcccgaa tggacgtaga atgcccgcaa gggcggctga cagagtaaaa cgtaatggat
    28261 gactgtggtg cggttttgca taatattgaa actaaatggc tttatgattt tctgaccctg
    28321 gaaaaatgcc gcaatttttc ccaggcagca gtcagtcgca acgtctcgca accggcattc
    28381 agccgccgca tccgtgcgct ggaacaggcg attggcgttg aattgtttaa ccgccaggtg
    28441 acgccgctgc aactctcgga acaaggtaaa atattccatt cgcagatccg ccatctgttg
    28501 caacagttag aaagcaacct tgcagaactc cgtggtggca gcgattacgc gcaacgcaaa
    28561 attaaaatag ccgctgcaca ctctctttcc ctcgggctgt taccgtccat tatcagccag
    28621 atgccgccgc tctttacctg ggcaattgaa gctattgatg tcgatgaagc ggtcgataaa
    28681 ctgcgtgaag ggcaaagtga ctgtattttt tcctttcacg acgaagattt gctggaagcg
    28741 ccgtttgacc acattcgctt atttgaatct caattgttcc ctgtctgcgc cagtgacgaa
    28801 cacggagaag cactttttaa cctcgcgcag ccacactttc cgttactgaa ttacagccgc
    28861 aactcctaca tggggcgatt gattaatcgc accctgacgc gccacagtga gttaagtttc
    28921 agcacctttt ttgtctcttc gatgagcgag cttttaaagc aggttgccct cgacggctgt
    28981 gggattgcct ggctgccgga gtacgccatc caacaagaaa ttcgcagcgg gcagctcgtt
    29041 gtactgaatc gggacgaact ggtgatcccg attcaggcct atgcataccg aatgaatacc
    29101 cgcatgaatc cagttgccga acgcttctgg cgtgaactgc gcgagctgga gattgtactt
    29161 agctgacatt ctcgagcgtc gcactacgcc aaccgccatt atcccagacc agatcaaacg
    29221 ttagcggttc atcgtcgtgg tacagcatta tctcaaggct gatcgccagc gtgtggctgt
    29281 acgcttcttt cagattgacc tgcaacgccg caatccgtgg ctcatgaaat aacagtgcag
    29341 aacgcagctg acggcaatac cagctctgga gatgtttgtc gctctggtta tgcacttgcc
    29401 actcgggcat gcctgaaaca agtaatgacg gtaatgccga atgaggagaa ttaaaaagag
    29461 tgaggagatc gctacatatt gatttacgca gcgatgacgt tttattgcct gaaatttcag
    29521 gtaaaacagc ctgacgtgat tggcgcatca ttttgcgcac tcctgacgtt tagcaacata
    29581 tgcggcttgc cctgaacacc gggcaccact gtattgatgc tcattttcca tagccattcc
    29641 cttttgttca ttacatccat gtcagtctga gacaggcctg aatcctgtca gcaaagtata
    29701 aaaccttact tgcctatgaa tatctactga cttttcagga aaaaagtcat gatttgataa
    29761 aaatcatcaa actcaaaaaa aaaccgctag tatattctga atattccgaa tagttacgta
    29821 gatgaaaata cttgtcacca ccgctactta attccggtgt gaaattattt atttccgcat
    29881 aatcagc
//
LOCUS       DBBGLQ010000051        29434 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.051, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000051 DBBGLQ010000000
VERSION     DBBGLQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.051"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..1764)
                     /locus_tag="ACIYSB_003074"
     CDS             complement(<1..1764)
                     /locus_tag="ACIYSB_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HGC5430068.1"
                     /translation="MTDITANVVVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIPVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGAQQFK
                     FPNVLKYDPDQFEARLYDDVGSGNFPSKMGYIHNTLHATLRTLQHKLDDILSVKDFGA
                     VGDGLDHPLSEKFSTLSAAQMVYPFVTALTQTQDYAAIQAAINTAKFINAGVFLPSGQ
                     YVVNTEIVADYALSLYGEGSGGLRDIASTGHEESPVRGSVILSKVNPGRTLSINSTTG
                     FSFGMTLRDFAIWGVQDSCDVGLYTNHVGWTGIMSGLNIQRFPNQAWEVGYIQDTYVN
                     NCTFLLSGSQDKPAVHFLNDSNYIYFNGCHFEGTPYFIKMTNAWNIAFHHCHFEVART
                     VNGSLDDRFFYRSAPIDLGASYRIQFSENTFIPTDVGYLSSKLDVPRNQVPYFMTGSG
                     GNSISFSGDIFLAPEGSVDAMYLDCTAVSVLGCKFIRMSPSRECVHITDGHVSNCEIG
                     ISAAEDLTKLYGIYVGKGGVSNCTFGFYESDSGGKRNEGALLFGEGKANGNSYPDDDR
                     VYIFLDSPTKVDGYDGGDARFI"
     gene            complement(1929..4322)
                     /locus_tag="ACIYSB_003075"
     CDS             complement(1929..4322)
                     /locus_tag="ACIYSB_003075"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGC5430069.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEA
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQGGIMSDLGNGLA
                     ETWRGLLQAGINVANIPAELTDAVTSAAAWAGGKLGIGDGTYQPAPRVTTQGLERDFG
                     LQQGELTPQTTEGRVFAEALPYLTPAGVERAAAQAPTLAGRIAHGATRLLAENAVGSL
                     AANSAKDDAEALATDLGVGVLAGGAINAAGRGLGAAYRGVRGAITPEAQQAIRFAERE
                     GVPLHTTDLLQPTSRVGKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYD
                     PAVIIDSLKAKTSGIRRAAGNRLEQVQNAMAGVNILPARAIQQIDTEISNLQKLGKVA
                     DNETISKLQSYRDELVRNAGPDGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAI
                     QRVYKAMTDDINGAISQNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPE
                     VVNNMLFSKNKSEIKTLYNSVGRVGRAQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQ
                     TGITFKGQDAAYLKGLKNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAA
                     VSYGLLTRAYESEPFRNAMLRMANTPRGSTDFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(4323..5660)
                     /locus_tag="ACIYSB_003076"
     CDS             complement(4323..5660)
                     /locus_tag="ACIYSB_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HGC5430070.1"
                     /translation="MATWQQGINSGGFLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIE
                     RSGANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYP
                     DQIESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQD
                     VAQMYQQNPQQFGEFVDHLGMASLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQA
                     ITMRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAEANDLKRQEIQSRIAANNQ
                     KLQQKQQELNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQ
                     ARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNT
                     LNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            complement(5670..6350)
                     /locus_tag="ACIYSB_003077"
     CDS             complement(5670..6350)
                     /locus_tag="ACIYSB_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGC5430071.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANRPSGLQSALGGAMSGAASGAMIGSVVPGIGTAVGAIGGGI
                     IGGLGSLF"
     gene            complement(6337..6804)
                     /locus_tag="ACIYSB_003078"
     CDS             complement(6337..6804)
                     /locus_tag="ACIYSB_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HGC5430072.1"
                     /translation="MEIKLIDNPVKLAEFLNNPANTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(6804..7652)
                     /locus_tag="ACIYSB_003079"
     CDS             complement(6804..7652)
                     /locus_tag="ACIYSB_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HGC5430073.1"
                     /translation="MADSNLNEPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILVDHEIRLASAEAKIHDHETRITNAEAAIVGLD
                     SRLTTAENDIDYLTDEVVAIQNTLSNHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTANNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(7652..9070)
                     /locus_tag="ACIYSB_003080"
     CDS             complement(7652..9070)
                     /locus_tag="ACIYSB_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HGC5430074.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYAADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGELQFDISSQYGLQQEHMLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            complement(9071..9571)
                     /locus_tag="ACIYSB_003081"
     CDS             complement(9071..9571)
                     /locus_tag="ACIYSB_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HGC5430075.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCNSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            complement(9549..9779)
                     /locus_tag="ACIYSB_003082"
     CDS             complement(9549..9779)
                     /locus_tag="ACIYSB_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430076.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVADPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            complement(9824..11119)
                     /locus_tag="ACIYSB_003083"
     CDS             complement(9824..11119)
                     /locus_tag="ACIYSB_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HGC5430077.1"
                     /translation="MALNEGQLVTYALDEIIETVQNLTPMASKVTKYTPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLSGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGEQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     TGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFTGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            complement(11119..12030)
                     /locus_tag="ACIYSB_003084"
     CDS             complement(11119..12030)
                     /locus_tag="ACIYSB_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HGC5430078.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDNAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYDAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            complement(12044..14209)
                     /locus_tag="ACIYSB_003085"
     CDS             complement(12044..14209)
                     /locus_tag="ACIYSB_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGC5430079.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPVVRKLVAEMRRNPIDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDSLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEKKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRHV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANSQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELHEYLKLVDQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            complement(14210..15709)
                     /locus_tag="ACIYSB_003086"
     CDS             complement(14210..15709)
                     /locus_tag="ACIYSB_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGC5430080.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENDEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            complement(15687..16175)
                     /locus_tag="ACIYSB_003087"
     CDS             complement(15687..16175)
                     /locus_tag="ACIYSB_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGC5430081.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWVEANPL
                     WEMKAFSYQGEVIQEPIAKMRAMTITGLTLFIDVTLETWRTYRLREDLSEVVTRAEQV
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            complement(16211..16453)
                     /locus_tag="ACIYSB_003088"
     CDS             complement(16211..16453)
                     /locus_tag="ACIYSB_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HGC5430082.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            complement(16557..16937)
                     /locus_tag="ACIYSB_003089"
     CDS             complement(16557..16937)
                     /locus_tag="ACIYSB_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HGC5430083.1"
                     /translation="MNDQQIEKEIVEKGKTAPRITPQHIEDVIKSEHYFTAYDGRNGA
                     ISSNEYCGREKPEEGDRDLSPLKLLTFCVLVLKNGFTVTGESACASPENFDAEIGRKI
                     ARQNAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(17169..17693)
                     /locus_tag="ACIYSB_003090"
     CDS             complement(17169..17693)
                     /locus_tag="ACIYSB_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGC5430084.1"
                     /translation="MNNHSVIPAFDFREMVQAKNGEVVTTSRKIAKYFGKRHGDVLRK
                     IEQVKADCSREFSQRNFASADYIDEQGKVRPMYSLTKDGWIMVVMGFTGKAAAAIKES
                     YIAAFNWMAEQLSRRMAIGEEMQHRYAIKETRSKLKGTIGSRLMNERKKEKRVLAVEH
                     EYILQVTQPELLIN"
     gene            complement(17896..18048)
                     /locus_tag="ACIYSB_003091"
     CDS             complement(17896..18048)
                     /locus_tag="ACIYSB_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430085.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RSKGLR"
     gene            complement(18036..18473)
                     /locus_tag="ACIYSB_003092"
     CDS             complement(18036..18473)
                     /locus_tag="ACIYSB_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGC5430086.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLR"
     gene            complement(18470..18946)
                     /locus_tag="ACIYSB_003093"
     CDS             complement(18470..18946)
                     /locus_tag="ACIYSB_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGC5430087.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPQLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(18930..19253)
                     /locus_tag="ACIYSB_003094"
     CDS             complement(18930..19253)
                     /locus_tag="ACIYSB_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HGC5430088.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(19496..19571)
                     /locus_tag="ACIYSB_003095"
     tRNA            complement(19496..19571)
                     /locus_tag="ACIYSB_003095"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19536..19538),aa:Thr,seq:tgt)
     gene            complement(19577..19651)
                     /locus_tag="ACIYSB_003096"
     tRNA            complement(19577..19651)
                     /locus_tag="ACIYSB_003096"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19616..19618),aa:Asn,seq:gtt)
     gene            complement(19744..20232)
                     /locus_tag="ACIYSB_003097"
     CDS             complement(19744..20232)
                     /locus_tag="ACIYSB_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGC5430089.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     RVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPVLDSAFKNRKLVEKIKH
                     VA"
     gene            complement(20276..20887)
                     /locus_tag="ACIYSB_003098"
     CDS             complement(20276..20887)
                     /locus_tag="ACIYSB_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGC5430090.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTVEECRAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(20880..21050)
                     /locus_tag="ACIYSB_003099"
     CDS             complement(20880..21050)
                     /locus_tag="ACIYSB_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGC5430091.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEEDDG"
     gene            complement(21089..21229)
                     /locus_tag="ACIYSB_003100"
     CDS             complement(21089..21229)
                     /locus_tag="ACIYSB_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGC5430092.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKLIQKESDVKTFNYTA
                     HL"
     gene            complement(21226..21756)
                     /locus_tag="ACIYSB_003101"
     CDS             complement(21226..21756)
                     /locus_tag="ACIYSB_003101"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGC5430093.1"
                     /translation="MIKHKSETPKEVRDCWQTPLWLFDALDIEFGFWLDSAASDKNAL
                     CAHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMS
                     VGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTV
                     SKAALMAIGQGVRRAA"
     gene            complement(21794..22240)
                     /locus_tag="ACIYSB_003102"
     CDS             complement(21794..22240)
                     /locus_tag="ACIYSB_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGC5430094.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGKRLAPEDWKDLFTALWLKTKKMEQRSAPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            complement(22273..22428)
                     /locus_tag="ACIYSB_003103"
     CDS             complement(22273..22428)
                     /locus_tag="ACIYSB_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430095.1"
                     /translation="MTNQQQIEFILEQIRKMREKNQPDMMELWRRQREEWHRHIFGER
                     KRDGWSL"
     gene            complement(22439..22654)
                     /locus_tag="ACIYSB_003104"
     CDS             complement(22439..22654)
                     /locus_tag="ACIYSB_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430096.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFRLNNEFQRNRIIRNLNLK"
     gene            complement(22740..23009)
                     /locus_tag="ACIYSB_003105"
     CDS             complement(22740..23009)
                     /locus_tag="ACIYSB_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430097.1"
                     /translation="MNKKQLAILEKAWDAQISCALKEQALPIIQTKSKIARQLCDGGF
                     LNEVEITRQMVTFKGYEINHHGIAAYCSHLPDDVDIDEMEREMKQ"
     gene            complement(23010..24890)
                     /locus_tag="ACIYSB_003106"
     CDS             complement(23010..24890)
                     /locus_tag="ACIYSB_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HGC5430098.1"
                     /translation="MTPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNLSGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVI
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVALPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDAINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKTEVVGHMALEAMRQGVKTCIA
                     SLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFD
                     YARRRYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDS
                     EEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPAS
                     VLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVT
                     EY"
     gene            complement(24998..25858)
                     /locus_tag="ACIYSB_003107"
     CDS             complement(24998..25858)
                     /locus_tag="ACIYSB_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGC5430099.1"
                     /translation="MSNLATVTHLRPSQRPVERRVAEVEDGYTRLANALYEELIGADL
                     TKNQSKVAHAICRKTYGYGKKMDRISDSQLAQITRLPRQKVNKAKNELIAMKVILREG
                     QQIGPNKNIEEWQIEGCHYSGDNVTALVTKSVTKTVTALSPKQGHTKETITKEKRNNK
                     NTMSESVRTECEKSPDRHEETDKAFEEIFWCAGMRKAGKKNAASAFRTQFREWRKTTR
                     GTASEFATMLAEDIACRNGKQFGFDRLLPSSYLNGQRWNDEKPETIQPQSKPSSAITV
                     SKTGYVFFDR"
     gene            complement(25851..25997)
                     /locus_tag="ACIYSB_003108"
     CDS             complement(25851..25997)
                     /locus_tag="ACIYSB_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HGC5430100.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            complement(26030..26323)
                     /locus_tag="ACIYSB_003109"
     CDS             complement(26030..26323)
                     /locus_tag="ACIYSB_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HGC5430101.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            complement(26433..26657)
                     /locus_tag="ACIYSB_003110"
     CDS             complement(26433..26657)
                     /locus_tag="ACIYSB_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5430102.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            26769..27476
                     /locus_tag="ACIYSB_003111"
     CDS             26769..27476
                     /locus_tag="ACIYSB_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGC5430103.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRESSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            27517..27813
                     /locus_tag="ACIYSB_003112"
     CDS             27517..27813
                     /locus_tag="ACIYSB_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430104.1"
                     /translation="MEFLIVFVVVLIIILFVLLSISKKLSQMIEHSSNRAKEEEHLID
                     IKEILSDIKITLDEIKYTTDLIEQYKIPTPNERKAIDQYRIDLEIDEMLSNRKD"
     gene            28248..28553
                     /locus_tag="ACIYSB_003113"
     CDS             28248..28553
                     /locus_tag="ACIYSB_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430105.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKAEEVERKQNRIYYRKPRSEMGVTCVGRQKMKLGSKPLYEG"
     gene            28557..28688
                     /locus_tag="ACIYSB_003114"
     CDS             28557..28688
                     /locus_tag="ACIYSB_003114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430106.1"
                     /translation="MSYQIQYRSCGRWVCGNSFKTKEQAEQYAKQTGQDYQIVKTRR"
     gene            28690..>29434
                     /locus_tag="ACIYSB_003115"
     CDS             28690..>29434
                     /locus_tag="ACIYSB_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430107.1"
                     /translation="MPGFTSKFIPRREFKHNPERLNVISSGGGTQSNAIICLVHAGVL
                     PKPDIIVMSDTEREASNVFEYQKKYIAPICEDMGVDYLIVPKSKFATYDIDGPDPDVP
                     LPGYFTEYEGRDSKGMCAGKQPAFCSIKWKTEVIQRFLNERYGEKYLTARGVDSWMGI
                     SIEEAKRRAKYPTGKWQRRYPLIEMLMTKQMAIQCVEDYGLPTPPASLCWMCPNRDDD
                     LWLYMKNNVPSDFERACQHEKEIQMKWPHL"
BASE COUNT         6942 a         7437 c         6862 g         8193 t
ORIGIN      
        1 aatgaatctt gcatctccac cgtcgtaacc atcaaccttc gttggtgaat ccagaaaaat
       61 atacacacgg tcatcgtcag ggtatgaatt accgttagcc ttcccctctc caaataatag
      121 cgcgccttca tttcgtttcc caccggagtc agactcatag aaaccaaagg tacagtttga
      181 aacaccgcct ttgccgacat agataccgta caattttgtt aggtcctcgg cggctgaaat
      241 ccctatctcg caattactta catgtccgtc ggtgatatgt acacattcgc ggctgggaga
      301 cattcgtata aatttacacc ccaatacact aacagctgtg caatccagat acatagcatc
      361 tacactacct tctggtgcca ggaatatatc cccagagaag ctaatgctgt tacctccaga
      421 acccgtcata aaatacggaa cctggtttct tggtacatct agtttcgacg acaggtaacc
      481 tacatcggtc ggtatgaatg tattttctga aaattgaatt ctatagcttg caccaaggtc
      541 aatgggtgcg cttcggtaaa aaaatctatc atccagtgag ccgtttactg tacgggctac
      601 ttcgaaatgg caatgatgga acgctatgtt ccacgcatta gtcattttaa taaagtaagg
      661 agtaccctca aaatgacagc cattgaaata gatatagtta gagtcgttaa ggaagtgcac
      721 tgcaggttta tcttgagaac cgctgagcaa aaaagtgcag ttgttcacgt atgtatcttg
      781 aatatagccc acctcccacg cttgatttgg gaagcgttgg atatttaaac cggacataat
      841 ccctgtccaa cctacatggt tagtatacag acccacgtca caagagtctt gcacacccca
      901 tattgcgaag tcgcgcaatg tcataccgaa tgaaaaacct gtagtgctat taattgacaa
      961 cgttcttccg gggtttactt tagacaatat tacagaccca cgaacaggcg attcctcgtg
     1021 acctgtagag gctatgtcac gcaaaccacc gctgccttcg ccatataagc taagagcata
     1081 atccgccaca atttctgtat ttaccacgta ttggccagat ggcagaaaca cgcctgcgtt
     1141 aataaattta gcagtgttaa tcgcagcttg tatcgcagcg taatcttggg tttgtgttaa
     1201 tgcggtaaca aatggataaa ccatctgagc agcacttaat gtgctgaact tttcgctaag
     1261 aggatgatct aaaccatctc ctacggcgcc aaaatcttta acagatagta tatcgtcaag
     1321 cttatgttgc aatgtgcgca atgttgcatg caaagtgttg tgtatgtatc ccatcttgct
     1381 gggaaaattt ccagaaccaa catcatcata taatcttgct tcaaactgat ccggatcata
     1441 cttcagcaca tttgggaatt tgaactgctg tgcaccatac gcatcataaa cagccataga
     1501 atggccttgc tcagttacga atttggcaat ctgcccgtta tataccgggt aaccagcagc
     1561 attaatgatg attggttgcg atacaggaac gtgagaacca tcttcgttct ccacataaac
     1621 cggaatctgg ttttctggat ttacagggtc agtgtcaatc ttcccgatat aaattttgcc
     1681 attggcaact gctttaaaag aacgcgccat agtgaagagt tgcgatggca tacttactac
     1741 aacattggct gtaatgtctg tcatttaatt tgctccggac gtagcaatgc ctgacaagat
     1801 gcaacttgcc cagcattgca ctaatgtcag ttatgatttg ttaattatga gatgagtcta
     1861 tgcaaagaga tttgttgaat attgcgttct acatatttgg tttttgcacg ttcctggtgt
     1921 ttgaaaagct attctgacaa cgcatcagac ttagccccct gcgtcagagc gttaattgcc
     1981 ttttgtgcct gctgcattgc tttctcaaag tctgttgatc cgcgtggggt gtttgccatt
     2041 cggagcattg catttctgaa tggttcgctc tcataggcgc gagtaagaag tccgtagctt
     2101 actgctgcgc cagttgtcgc cgggttcatt gccgtcccat acccgataat gaacgggatg
     2161 gtttgctgcc ctgttggtgt tgttactgcc gcttttgcag cctgctgcgt ggattgcagg
     2221 taatttttca atcctttcag ataagcagcg tcctgcccct taaatgtgat gccagtctgg
     2281 ttttgcagga tgttaagctg tcgaaggaac tggtcaggtg aaccccctga tttctccatc
     2341 gcctttccaa tgatgccatt gcgcatttgc gccctgccaa cacgaccaac tgagttatac
     2401 agcgtcttaa tttccgattt gttcttgctg aatagcatgt tgttgacaac ttccggcgtc
     2461 aggtcgcctt tcatgagaac attcttcagc ctggtattct ttagtttcgc cgcttcgtca
     2521 gcgtagacgg cattggcctg ctgatattta cggagagtat cgttaccaag attctgacta
     2581 atggcaccat tgatatcgtc tgtcatcgcc ttgtaaacgc gctgaatggc agcatcggaa
     2641 cggtttggta acactggtcg ttcccccttc acgtccattc tgaactggct gcgcagatcg
     2701 cttaattgct tcaaatcaag atttaccgga ccatcagggc cagcattgcg aacaagctca
     2761 tcacgatatg actgaagttt tgaaatagtc tcgttatcag cgaccttacc aagcttctgc
     2821 agattagata tctcggtatc aatctgctga attgctcgcg caggcaggat attgactccc
     2881 gccattgcat tctgaacctg ctcaagacgg ttccctgcag cgcgacgaat tcctgatgtt
     2941 ttcgctttaa ggctgtcaat aataaccgct ggatcatact cgccgaattt atcagcaaat
     3001 ctctgcacca actggcttct cgcttcctgt tgcgttgctc tcattccgct tgtgccagcc
     3061 agagggatat tttctgctgt agtctgcgcc atttttccga cgcgggaagt gggttgtaac
     3121 aggtctgtgg tgtgcagagg aactccttca cgctctgcaa atctgatagc ctgctgcgct
     3181 tctggcgtga tagcaccacg aacgccacga taagcagcac ctaatccacg tccagctgcg
     3241 ttaatagcgc cgccagccag cacaccaacg cctaaatcgg tggcgagtgc ttccgcatca
     3301 tctttcgcac tatttgcagc aagtgatcca actgcgtttt ctgctagaag gcgagttgcc
     3361 ccatgagcaa ttcgaccagc aagtgttggt gcctgtgctg ccgctctctc aacgccagca
     3421 ggagtgagat aaggcaatgc ctcagcaaat accctgccct ctgtcgtttg tggagtcagc
     3481 tcgccttgct gaaggccaaa gtcccgctct aatccctgcg ttgttactcg tggtgctggt
     3541 tgatatgtac catcgccaat gccgagttta ccgccagccc aagccgccgc gcttgttaca
     3601 gcatcagtga gttcagcagg tatgttcgct acgttgattc ctgcctgtag taagccgcgc
     3661 caggtttcag caagtccatt accaaggtca gacattattc cgccttgctg ctgaacaggt
     3721 ttcggtgcga cagaacttac gggttgaggt ggctgctgac tggctgcctg ctgctcaatc
     3781 tgagcaaatg gattatttgg gtctgactgc acgcctgatg ccgatacttg ttcggatgac
     3841 tgtgcttcca gttgtgcaaa cgggttgtta gggtcttgct gaggatgtac ctttgcggag
     3901 gtggcgcgct gttcgactgt tgagtctgtc accgggtcac ccgcccattg agcaaagcga
     3961 tcatctacgt aaccgcggcc ttcaggtcct ggcgtatatt caccacgctt tgccttcata
     4021 acgttgccgg gaccgtcgtg ataagcctga agagcgtcac gccagttacc aaactgctgg
     4081 tacatctttg ccagatagcg cgcgccagcg tcagcctgat attcggggtt ttgcatttgc
     4141 tcatcggtat aacccatatc acgccatgtc ccaggcatga cctgagtcaa tcctacagcc
     4201 ccggcggagc ttactgcggc agggttgtaa gaagactcct tggcaccaag tgcagtcatc
     4261 aacccttctg gcacaccgta acgtgcgcca gcctgctcta acaaatcacg gtaattagcc
     4321 atttactgcc ccaaagatgg aagatatccg tagcgattaa tgaagtcgat tgacagctcg
     4381 gggtgctgct tcaggtaatc tatagaagcc tgaggcgctt ccactcgctt gataccgttt
     4441 tgctgaacgt acttaccaac cgcctcatta cgcttctggt tgagcgtgtt caggatgacg
     4501 ccagcgttgc gacgaaagga ctcctcgctc tgcgagtttt gcagagaacc aacagcctgg
     4561 tcgagctttt tgccctcggc atcagaaagt gcacccatgc ctcgcatggc ctgaaccgct
     4621 gtcaggtatg cttgggattt aaaggtatca agtcgtgcct gagtgtctgc agcctgtgag
     4681 cctggaacgt tggggattac cccacgtaag cctgtaatgc tcttaagtga aggagaacta
     4741 acgatatcgt tcagagtgaa catgctggtt gtgagggtgt tgatgccgtc tttgtagcca
     4801 tcatttagct cttgctgctt ctgctgcaac ttctggttgt tggctgctat gcggctctgt
     4861 atttcctgtc gcttcaggtc gttagcttct gctgatagca tccggtcaag gcgcttattt
     4921 tcgttgttaa tgcggtttgt ttctgcgtcc agattaatgc gctgctgacc taaattcgcc
     4981 tggatatctt gtccgcgcat tgtgattgcc tgattccgag cggcggtttg cgaatccaga
     5041 tcctgaccgc gcatggtcac ctgacgaccc tgcattttat cctgaaggtc aaagtatttt
     5101 tcggggccga ggctggccat ccctaggtga tcgacaaatt cgccgaactg ctgcgggttc
     5161 tgttggtaca tctgagcgac atcctgagga ttaacgccaa cacgagctaa ctcaccggcg
     5221 ttgttttgca gccatgattg cattgcttct ggagacgatg atgcaaggcg tgcgccagcc
     5281 gctaaggtgc cgatagaatt acgctgctct tcatcaatga atcccatgcc tttacgaacg
     5341 gattcaatct ggtctggata ttgagtagcc aactgacgca aagcaccgcg atcaccagac
     5401 gcataagcat tagcgtacgc ctgctgaaat tctttctgcc gctgagcctg cttttcctgc
     5461 tgaaacaccc ctgcaatacc tgaaaggcct tgcaaagctg tcagcccaac attgttagcg
     5521 cctgaacgct caatatcatt gttctgcctg ataagctgaa gcgtgttgcc gatgtcattt
     5581 acgctcggag cgtttgagtt gacaccaccg ataccagcaa gaaaaccgcc tgagttgatt
     5641 ccttgttgcc aagtagccat attcccacct taaaacaatg atccaagacc accgataatg
     5701 ccgccaccaa tagcgccaac agccgtacct attcctggca ccacagagcc aatcatcgcc
     5761 cctgatgccg caccgctcat ggcacctccc aaggctgatt gcagtcctga tggtcggtta
     5821 gcattcgccg cagatgctgc cgcctgctgt tgatacaatt ggctcacgtt gttagcgtag
     5881 ttctgtccgg cgtttgcctg acctgtaaga gcaccaaggc cgatatttgc cagattgttg
     5941 tagttgttca tctgacctga cagccagttt tgaccgagtg taggtgcgat tgctgccaac
     6001 tggtttccgg ttgctgtaga gcccaatcct cccgttgcct ctgctgcggc cagactctga
     6061 tagcgcgcct gacctgcaag gtctttgtac tgctgggagt tgtaatactg gttaagcgcc
     6121 tgaccttgcc cctgaagaga ggaaagattt tgcaactgtg atacatactg ctgagcgagt
     6181 ggtgtgaacg gtgcaaggtt ctgcatgttc gtttgccaca tttcacgctg caattcgatg
     6241 cccttttcag ttgcgcgagc ctgggcttta gagccggagt cactgccgcc tttcatatac
     6301 ccattcatgg gaagcaattt attcttgaag ctttcgctaa gtactaacat ttaatagctc
     6361 ctcatatttc gaacgaggta attgatagag ggtgattcct accggttttc cgttactcat
     6421 gtacgcatca tcaaggtgac caacacgggt agcgccaagc aaacggataa ttgcccgtcc
     6481 gtatttcgtg gtgtcaggaa ccatagtgat gctgttaagg aatggtgaat tttcgagaag
     6541 ccatttgcag aataatcgat gcccttgcag tgcatattca ccacggaatc cggggtcgta
     6601 caccgcatgg cattcaacaa cgctatgcca gaagttacgc acttcatgaa cgccagccag
     6661 cactaatcct tcgtagatgc cgaggtatac cgcatcaggc ttgatgtagt atttgtctcc
     6721 actatctacg atatttcccg tgtttgccgg gttgttgagg aattctgcaa gcttcaccgg
     6781 attatcgatg agctttattt ccatcactgc tccgcaatga ttttgatggt tgtggcagta
     6841 aacgccgcac cattagactg aatggttaac gtactgccat ttgtggcaag aaagccgtct
     6901 ttatccacgc tgaagaacgt agctaacagg atgttatcgg ttgttgtcgc cgcattacga
     6961 ctgctcacaa gtgtgtcagg aacagagccg gaaaaggtta gctgcattga tctgttggca
     7021 gttccgctgg gccacgtccc gacgatcgac agcttgaaga acaaggtttt gttctcgttg
     7081 aacacaacca tcttgttgtt agcagtgtcg aagaatggtg ccaacgtccc ggatgacggc
     7141 gtgagcgttt tcagcaggct aacaaggttg gtcggcgctg tcgggatggt tacagatacg
     7201 ccagagtaaa caacctccga cttcttgcga gtagtggcat actccagagc atcgatgcgc
     7261 gtttcatggt ttgaaagcgt gttttgaatg gcgacaactt catccgtcag ataatcaata
     7321 tcgttttctg ctgtcgttaa tcgagaatca aggccgacta tcgccgcttc tgcgttagtg
     7381 atccttgttt cgtggtcatg tatcttcgct tcagcagatg ccagtcgaat ttcgtgatcg
     7441 accagaatca catcctgctc atcgttcctg acttgtgcgt cataagcgcc ctgtccggcc
     7501 tcgttggcct tgttagccac gttgccaaca tcagtaccct gtgcgataac gtacagcaga
     7561 tacgactgcg agaagatatt gcgtggaaga actgatgtgt cgagtcgtgt agcctgaatg
     7621 attaccggct cattgagatt cgaatcagcc attactcaat ccttatctgg cagccagaca
     7681 gagtgacagg tgacttcgtg ataacgcgca atttgaagcc gacatttttc cttattcgcc
     7741 cgacgcgctt ccacaaaaca cgcttgtcgt aaacgaacgg ttcattctgc tcaatcatct
     7801 gctcacgacc gtaattgatg ccgtcagtgg ttgcagagag aaaaaggcgg tcagcatact
     7861 gcgcaacgcc agttgacgat tcaacttcaa ggtcgaaaac tctggcgtta tccgctttga
     7921 acagtggagt aaacagcatg tgttcttgct gtagcccata ctggctgctg atgtcgaact
     7981 gcaattcccc ggtcacggat tccagcttat cgccgcacgt tatctgattg ccttcgtaaa
     8041 tgaagtcgat agcgcggtac acatcgtcgt acaggcctgt tttcagtaca caccattgcg
     8101 gaccattagc acttgaagat gcgtcgtaca cgaggacatg gcgcggaaga tggataatca
     8161 gcagctcatg cgcatcaaat cgcagagact ccattacgcc atcagccagt tcatcagcag
     8221 cgtatgagcg aaggattttc tcaatgctcg cgctggcgat tggtgatacc tgaccggagc
     8281 cgatgatgta cacagacggc gcacctgttg ccggattgct gatgaacgca tatgaatcag
     8341 caaacggcgt tttgcagtaa gtcccggcaa tgcctttctg caccatcagt gatggctgtg
     8401 cgacatacaa agcggcacca acggtggttg caccagtcag ggagaaatat tcaatcgtcg
     8461 atgaaccaaa gcagacgatg aagtctcgcc atgtcccgat accgatgatg ccgtcaggct
     8521 gagactcggc acgatattgt gcgctgtaac ggtcagggtg cgattcgtct tcaaggtcag
     8581 tgataaacca tgaatcagtg ccgtcttttg accacgcata acgcccacgt aaacgcgtaa
     8641 tatcacgaac agaacctaac tcatactgcg tgaatccgct gtctgtaggc cagtttgaga
     8701 cggttttaac cgtgccatca tagcgatact cgaccagttg accattaacg cctaccgcct
     8761 gtgatgtccg accatgcgcc attgatacac gaccacttcc ggcaacatca ccgacttcgc
     8821 tttcgccttt gtagagcttg ccgccacaca cacgatagac agcattctgc gccatgttgt
     8881 actcgacgcc tcgagataca ccgttcacat cagaacgttt ggcaatgccc gggaatgagc
     8941 gaagatatcc gctgctgttg aggatttctt tgggtgtagc caacatattc actggcagat
     9001 agtcgatata gtcggcgttt cgaaagtctt tgccgacacc tttcataagc ggaagttgct
     9061 gaatcggcat ttattcgctc ccgttatcgc aaggttcctt ccggtggaag taattccaac
     9121 cattccactt cgccaactgg ttaccactac caacaggcat acggtttgga taaccggaat
     9181 tacatttagc ggcttttgct ctgtccattg cagacagttt gacgagtcgc tctttcccgt
     9241 atctggcagt ggttataagt tttgctgacg cttccagcgc ataatctgga gcaatgcggc
     9301 aggcaaggtt gaaaatgacg gcatttatag cgttatttga taagccgtgc tcatcgcccg
     9361 gatctggagc gacatctgca tcagcgaaaa tgtagccaac gttgatccca ggtgacgcat
     9421 caccgccaag ccattcagcc atcatcattt caaggtcgtt gacgccgtct tccatagact
     9481 gcggttcgac atctgttaac gtggcatttg atgccacacc gagcttacgt aatgccgcaa
     9541 ggactaaatc acccttcgtt gtcaggttca tctgctgccg ccttaggttt tcgaccaggc
     9601 tttttacgct gtttttcttc tggctctgga tctgcaacat ccttcaaaag gtcatcagga
     9661 tgtgaaaacc aaccagcatc cagatattcc tgaagctctt cggctttcac gatttcaaag
     9721 tcgtagccaa cgcctttcca cttcttcatg tcgccatgac gaaagatcat gtgtgtcatg
     9781 cttgtctcca gataaaaaag ggagccgaag ctccctctgg ttatcacgcg gtctggttag
     9841 gcagaccaac accaattgcc tctggtcgta cagcacatgc tgaataccac acagcaatac
     9901 ggcacttacc agacagagtg ttgatatcac cctgcgttgc gaagatgccg ttaacaccaa
     9961 taccaggaat gctgaaggaa gacgttttca tgccagtaaa cagctcatgg gttaccggga
    10021 tcggctgaga cagcagacgg attgagtcat cagcccagaa cacgttagcg gtggttgttg
    10081 ccacgttcag aacgtttacc ggagtggtat cagcaagaga ggtgtttacg ttagcgtaag
    10141 ccttctcttc ttttgtcagt gacgcgtcat ccagcgcaat aggcttcggc gtgatttcga
    10201 tgtgagtacc atcgatcaca cgggtgattg agaaagtcgc gtcatcagtc agcacgttct
    10261 tcgccatctg agacaggaat ttcacgccag tgaagctgat tttgtcgccg cgcttaaatc
    10321 cggtggtgga ggatacggtc accgttgcaa cacggttgtc gacgttctct ttgttgccat
    10381 cggtatcaag ggtgtatgcc tgcggcttaa acttctgctc accagaaaca gttacaccag
    10441 tagcggtgga cttggtaact gccggaagtt tcggtgagcg aagaatttca tcaaagccag
    10501 caatctgacg ctgaatagta ccgttgcgat aagcttcttc aggaacgcga ccaaagatgt
    10561 caccatcgac caggttgcgg cctgctttgc ggtaatcgtc agggttcagg aagtaactga
    10621 tgcccatgtc gcggttgagt tctcgggaga acatcaggcg ctctgcatca gacacaaaat
    10681 cccagccaga caggccagta gatggaccaa ttgcgcgggt atcgtgaaca acaagtgagc
    10741 ccatttcagt tgcctgtttg gcaatcgctg actcaatgtt attcgccagt tttttggcag
    10801 atgcctggat gcggcgacgg taagaacgct catcacgcag gtcatctgca cgaagctcga
    10861 agaaatcgtt atccggatcg cccatgttgc atttcacgga cagctccaga atcccggttg
    10921 cgttgccagt taaatcccag ccagtctgag ttggcgcttc ctgctcaaca ggcatccaca
    10981 cggtgttgct tgaacgctgc atggattctg ccggaggggt gtattttgtc actttggacg
    11041 ccattggcgt caggttctgg acggtttcga tgatttcatc cagagcatac gtgaccagtt
    11101 gaccttcatt taatgccatt atcgaattcc tttattcagt tgcgccttga gcttgcggta
    11161 cgtctctaca tcccctttgt ttgctgccgc ttccatctgc ttttcaatcg cagagatatt
    11221 tgcagcaaca gcgtgtccct gaatgggttc atcaggtagc ggggcttctg aaacaggttt
    11281 ggctcgaggc ttgagagtta aacgttctga cagtcgagtg agttcaatca acgcggattg
    11341 cccgtccatc gccagcaact ggcgtgtctt ctcaggatta gcaccaaggt gatacatgag
    11401 tgcagcggat ttctccggga agaggcgcat gatgtcggca ccgactgctg gcggcaccaa
    11461 ttgcatgaat gcatcctctt tctcctgata gtcagggata ttgagctttt ccgctgcgtc
    11521 gtagtgctta cgggctgcct cgacgtattg cgctgattgc tgggtgaact cctgagtttt
    11581 gcgaccctgc tcggcgacag cctggcttcg tgcgtccata gccttgatct gccattcact
    11641 gtttgcctgc tggaaggcag ccagtgcgcg gctctggtca tagtcgtact tagccagtgc
    11701 atcttcggaa agataatcgt tagggtctgg ttgttttggt aactcagggt tcacccgcag
    11761 gtgctccggc aactctccac gcttaaccgc ttccatctgc tgctcaagct cacgctggcg
    11821 tttgcgttcg atgcggcgac gggcaaattc agcattagtt gccgggtctt gttttggttt
    11881 ctcatcgtct ttcaggacaa tctcgaagcc ttcttcctga cctgcgttgt cgttggcatt
    11941 atcgacaact aagccatcag cagatgccgc tgcatgattg ccgggcaggg ttaattcttc
    12001 agaagcctga atgtcggtgg tttggtccat ggttaactct ctcttattga ggtgtctcgg
    12061 ctactccgcc ggaggggatt tgaacttgac gcataagatt cgcgaaatcc atgcgttgtg
    12121 aatgagtctg gtctgcatct ttaagaagca gctcagcgtt agcacgagca tctttgctgc
    12181 gctgttgctg gaattgatct acgagcttga ggtactcatg cagttctgcc tgcttgtcga
    12241 ggtccatatt gttgaagatt tctgcaatct tcgcggcgtt gagttggttt tgggcttcaa
    12301 ccttggcggc ttcaacctga atctgcgcct gttggttctc tgccttgagc aattcagcct
    12361 gaccttgcag aaggataccc tgcgcctgaa tttgctctgc tgatggctgc tgcggctgct
    12421 gttgagcctg ctgtaccatc tccatctctt caggtgtttc tggtttcttc agccccatca
    12481 tcaccagttg cttgttcgcg tactctcgca tcatctcgac gcctttaccg tcaagcagcg
    12541 tgaagtattg cagcatcagc atctggaact ctggagtacc ttgcggaacc ttggtgagca
    12601 actcctgaat ctctgcgcgg ttctgttcct tcatactctg gaaggatggt ccgacgtctg
    12661 tatagcactc atagcgaccg cgaatgtcgt tgagtgtgac cacattgccg gactggtaat
    12721 cgacaacttg cgcatagagt tgaacgtctt tctcgcttcc atcttcgagt gtcagcgtta
    12781 catgacgagg aacgtcataa atatcgttga ccattgaggc ataaatctcg ccatcacgtc
    12841 gcattgcggt agccaggtta tcctgaaaca cgtatgtctc aaggtctgcc cgcatgttca
    12901 gttgattaac ggtatcgaaa gcgacctgag agtttgctgc ctgcgcatcc acgccaagac
    12961 tagccacctc tttcactgcg ttggtggcag cctcaagcat ataagcgttg gcttgcggca
    13021 cttcagggtt ttccatgtag gagattggac caatcggcag gtcgttaccg ttttcatcgg
    13081 tcctgttctg cagatagtac ggatagtcat catttccacc gtacatgtat tcgtagcctt
    13141 cgatttgctc agggaagaag gtcggtttct tcttcggtga acgagcaaca atatcggcgt
    13201 tgaatgacat gatcatgtta cgaaggcgtt gaccgtcttt cgtcagcctt accactcctt
    13261 cgtagcactc cttgtcacca gcgaatgacc attcgccgta cacaggaacg attgggatat
    13321 gctctccggc tatcttttct cggtctttta gtatctgcgt gcaggtgatg atcgacttat
    13381 acacatgccg acgcttgacc ttacgctctg ctaccttaat gaatccacga ttagccaggt
    13441 cgtcgataac gtctttaata tcctgctggt aatagctgac cggctcacct gtcagcgggt
    13501 cgcggtagat gaagactttc tccttcttct cttctacctc gtaatactca gcgacgtaga
    13561 cgacatcatt cgatacccac ggaaacagcc atgtgtcgtt cggattctgg aaagatggca
    13621 aggagtccgg atcaataccg taatcctctg cgaactcttt ccagccattg cgcgacaagg
    13681 cgttaatcac cgtgcagtgc ttagcgtcgc tcttatccat ctgcttgctg tttgcgtccc
    13741 atatgacgtg tgagcaggct tcatggattg gcaggcgtcg aattacctga ttgttgcttg
    13801 ttgggtcgtt gtcttcgtac tgtgtgacca gacgccatgc accaacgccg gactctatct
    13861 gctcacgaac gccaacgtta acggcaattt ttgccgtgtt atggcgcata tcagtacgat
    13921 acatccccat caacacatcg gcagcatcag gattagcgcc gtcttttggt cgaaagagaa
    13981 cgtcgatagg attccggcgc atctctgcga ccagtttcct gaccaccggg cggacaacat
    14041 cgaattgtcc gcgatattgc agggtggtgt agtttgatag ccagtcatcc cattgcgaca
    14101 ctcggctaaa atacaggtca tttgtcgcct cggttctggc ttcatcgctc gccatccagt
    14161 ctgcgtcaaa cttacacaga atggaattga gtctgttttc gtcggccatt taagttctcc
    14221 gtgcgatggg cctgattggg gctggtatct ttttctcttt tggttttttg atgtcgcgca
    14281 tcatttttgc gaagcggcgc atcatgtatg catagcgaac ggcggatagc acgtcgtcgt
    14341 taagcttgac gatcttcccg ttttcatcac ggtgatagag gcggaactcc tcaaagaatg
    14401 gctcacaggt gttgaatact ttgaagcgac catcaagcat catgtcgcgc aattcagtga
    14461 ttccaggctc aacagcatta ccgccatcag gccatgtcgc atgctcctgc aacatcataa
    14521 aaccagcgtc tgcatactgc cctttgagct gctcacctcc gcccttctcg tgctggtttc
    14581 cgtcatgagg ccatgcggtt ggcactttat gcgcccatga tttaacagct ccccacgcct
    14641 gaacggctgt cttttctttc gccttccaca cgcgtgaaac gtagattgtg tctgcgtctt
    14701 tatcccacca aagctgaacc tgcgcctgcg ggtgatccca tccgaaatcc ataccgccaa
    14761 tcacatagaa gtgatcagga cactcgaacg gctgacactt aatcgtctct tccggtatct
    14821 ggaagattcg tccgctaccc atcgtaggaa taccgcgagc acgcgcctct ctctcatgct
    14881 ctggatagga tgcgatgatt tgctctttct gttcgtctgt gtagtgctca gcgtcgtaga
    14941 tggtcatgtt gaccactttc tgcgacttgc ttggattctt caggaacttg gtaacaacgt
    15001 cagacatccc catcagcggg gtaaacgtca gaattgagaa ttgaccgtat ttgttggtac
    15061 gggtaagccc ttcgccataa atgctgtatg gtggttcttc gtcaaaccac acgccgtgga
    15121 ttgtgtcacc ctgccagcga gcgcggcctt gcgagtatgg cttgaagtag cagattgaaa
    15181 tgccatcttc aacgccatca gccgtgtgat gcttaaccag aagatgatca acaaggttcg
    15241 ggaagaaagg agacttcttc cagctaatga tgtcctcttt cggtattgaa ccgtagcccg
    15301 gttcatcatt ctcttcaata cgaccgcaca ggatgcgttg agtcgttttg gttacagtct
    15361 cgtttgtctc gccgccaatc cagaagacaa caggctcata gaaacgctta cctttccact
    15421 caccgccata tttaccatca gccggatatc cttttgttcc cggataacgt ccggtaaggt
    15481 gaaacgcgac ttcagcagca ccagtaaatg acttaccaag ctggttacca gccataaaac
    15541 atcgctctgg atagtcatgt ccggcgtcga tgaactcacg ctgtttgctg tatggagtaa
    15601 attcatatag caggtgtgtg ttccggtagt tctcttcttc ttcgagtagc tcgagcaatt
    15661 cgatttgctc ttcgtcgctc aggttatcaa gaatcgcgtc cagttccacg gttgaatagc
    15721 tccttgatac gagagcgtcg cttatcgcga tctcccttat caggtgtcac gtcttcaact
    15781 tgcgactgct ctttgaggcc caaatcacgg gcgatgatgt tagcgttgag aaggtcagcg
    15841 gctgcgccag agaatttctg gtcgtagatg acctgttctg ctcgcgtaac gacttcagat
    15901 aaatcttctc gcaggcgata tgtgcgccat gtttcaagcg tcacatcaat gaacagagtg
    15961 aggccggtaa tggtcatcgc tcgcatcttg gcgataggct cttgtatcac ttcaccctga
    16021 tacgagaacg ccttcatctc ccatagcggg ttagcttcca cccactcgaa gtattcacaa
    16081 caagcagccc acagcgcctc aggcgattcg aatttaggat ttcgcccatg actactgcgg
    16141 gcctcccaaa atcggttgcc ctttggtgct gccatattca tctcacttag ttgttatttc
    16201 aggttgagca tcatgctccg gtagtgaaca ggtctaacgc ttccttcgat ttacgcaccg
    16261 cttcgaatgt gcggatcgtg atatccgaat tagcgccgcc tgactggaag tgaattttga
    16321 atagttcaag cttcagctcg tcagttccaa tgaattgaaa tgcttcttct gcggctgcgt
    16381 tctggttcat gaccagtttg taaatctcta actggaattt ctgttcttca gtcatgggaa
    16441 taatctctgc cattgttggc tccgtttatc cgttaaaagg gatatcagtt aagttatccc
    16501 gtgtagggta taagccatta tcaaagccac tctgtaggga atggcttttg taataactac
    16561 tgttcgctta gcttctgctt cagcaagtaa ccttcgagca tccagatttt gtttacagca
    16621 ttctgccggg caatcttccg accaatttct gcatcaaagt tttccgggct tgcacaggca
    16681 ctctctccgg tgacggtgaa gccattcttc agcaccagta cgcagaaagt gagcaacttc
    16741 aatggtgata aatcacgatc gccttcttct ggtttttccc tgccacaata ttcgttgctg
    16801 gaaatggcac catttcgtcc atcataagca gtaaagtaat gctcgctttt aatcacgtct
    16861 tcgatgtgct gcggggtgat tcgcggtgcc gttttgcctt tctcaacgat ttctttttcg
    16921 atttgctggt cgttcataat tatgaccctg tagagtggtt gcttgattag gatgtctttc
    16981 catcagtccg ccaccacaaa gaatcttttt tgccataagg caggaggttc atctttcagt
    17041 ggctgccggt gttatttccc cacttactgg cttgggttgt ttcgtggtac tgccgttaac
    17101 tggtggccca gaataaattc cggtttcatt atcaagcccc cccgtagata ggctttgtaa
    17161 tgacatcttc aattaatcag cagttcaggc tgtgtcacct gcaagatgta ttcatgctcg
    17221 acagccagga cacgcttctc tttcttccgt tcgttcatta accgactgcc gatcgtacct
    17281 ttcagctttg agcgtgtttc tttgatggcg tagcggtgct gcatttcttc gccaattgcc
    17341 atgcggcggc tcagttgctc tgccatccag ttgaatgctg cgatatagct ctccttgatt
    17401 gccgcagcag ctttcccggt gaaccccatc acaaccatga tccagccatc tttcgtcagg
    17461 ctgtacatcg ggcgaacctt gccctgctca tcgatataat cagccgacgc aaaattgcgt
    17521 tggctaaact cacgcgagca atcagcctta acctgctcga ttttcctgag aacatcaccg
    17581 tgtcgcttgc cgaagtactt ggcaattttt ctggatgtgg taacgacctc tccgtttttg
    17641 gcttgcacca tttctcggaa gtcgaaggct ggaataactg aatgattatt catagcgtct
    17701 ttacctttta gaaagtgagc ctgtctcaca gaaaagccgc ccgagagagg tcgccaccta
    17761 taacggcatt tctcaggctc gcttactgaa aggctctcgt taatatgcgc gtgagatgcg
    17821 ctgtgaaatt cagatataaa aagccccgcg aatgcgaggc taaatcctgg tatttgtaat
    17881 gaactggctc ttatctcaac gcagcccctt actgcgcgcc agatgctcaa tatcaagcat
    17941 cagcaatgag atgtttaatc tggatttact ccagaagtga tcaccaccct gtctacagag
    18001 ccagatgtga aggatgatga gtaaaattat cgctatcatc gaaggcattg cgtccttatg
    18061 tattcctgca ggtagttaac ctgcgcggtt atcctgtcga ttccacttcg gagacggtaa
    18121 taattgagtt cagcatctgc tgtaagtctt gggctttctc catcgcccat gctgctggct
    18181 ccggtcgttg actttgcaca ggtggcggag acttgcaggc gcttacgacc agcagaaaca
    18241 tcagcacgga gactttcgat agtcgcgtta gcatcagcaa gctcctttgt gtatctggcg
    18301 tcgagttctg ctacatcacg ttgacgcttc tgcatatcag cgatgatgga tgtggcttta
    18361 tcgcgctgtt ctttgtaggc gattgcatta tcacggtaat gattaacagc ccatgacagg
    18421 caaacgatga tgcagataac cagagcggag ataatcgcgg ttactctgct catacctcaa
    18481 tctctctgac cgttccgcct gcttctttga attttgcaat caggctgtca gccttatgct
    18541 cgaactgacc ataaccagcg cccggcagtg aagcccatat attgctgcaa cggtcaattg
    18601 cctgacgaat atcaccacga tcaatcatcg gtaaagcgcc acgttcttta atctgctgca
    18661 atgccacagc atcctggctt ttgggagaga agtctttcag gccaagctgc ttgcggtagg
    18721 catcccacca acgggaaaga agctggtaac gtccagctgc ggttgattta agttgcgggt
    18781 ttagcgtgac aagtttgcga gggtgatcgg agtaatcagt gaatagctct ccgcctacaa
    18841 tgacgtcata accatgattt ctggttttct gccgtccgtt atcagttccc tctgaccacg
    18901 ccagcatatc gaggaacgcc ttacgttgat tattgatttc caccatcttc tactccggct
    18961 tttttagcag cgaagcgttt gataagcgaa ccaatcgagt cagtaccgat gtagccgatg
    19021 aacacgctcg ttatataagc gagattgcta cttagtccgg cgaagtcgag aaggtcacga
    19081 atgaaccagg cgataatggc gcacatcgtt gcgtcgatta ctgtttttgt aaacgcaccg
    19141 ccattatatc tgccgcgaag gtacgccatt gcaaacgcaa ggattgcccc gatgccttgt
    19201 tcctttgccg cgagaatggc ggctaacagg tcatgttttt ctggcatctt catgtcttac
    19261 ccccaataag gggatttgct ctatttaatt aggaataagg tcgattactg atagaacaaa
    19321 tccaggctac tgtgtttagt aatcagattt gttcgtgacc gatatgcacg ggcaaaacgg
    19381 caggaggttg ttagcgcaac ctcttgccac ccgctttcac gaaggtcata tgtagaaggc
    19441 cgcagcgtaa ctatcactga tgaattcagg atagccagtg gctacggctc agttatggtg
    19501 ctggttaacg gacttgaacc gctacccatt cgcttacaag gcgactgctc taccattgta
    19561 gctaaaccag catgtttggc gggacagcgt ggactcgaac cacgataaga aggttaacag
    19621 ccttccgtaa tgacctttat acgactgacc caaataaaaa aaagccaccg ttgcaactta
    19681 agagtcacta acggcagctt atgcgaatag tgttgctcat ttgctcaatg atgtcaacac
    19741 gttctatgct acatgtttaa ttttctctac aagtttccgg tttttaaacg cactatccag
    19801 aaccgggtaa atcataaaca acgaggcatt gaggatttcg tcaacttccc gtcgacaggt
    19861 tgcgagcgat ggtttttgaa tgcgcccgcc gccccggcat aacatcttgc gaggtcttgc
    19921 gacgcgatga tagtaagatg caatggcgtg cttggaagag ccgtgggcgt agtagctgag
    19981 gaggatgcca aaggctttct tgtcaatgta catgacggaa tcgacgacct gagaaatcaa
    20041 cattccatca tcatcattac acattggcct tgtcataact cttcccggct ctacgctctc
    20101 catgaactta gctattacgc tgctcatgcg cttttccaga cgacctgaat aaacccatgc
    20161 gccccacagt tcaagccagc cattcagcca ctcgtgctgt tctttggtga ggtttagttc
    20221 tcttatgccc atgcgccttc tcccttgtga tctggaatgg tttttactga gaacgtcatg
    20281 cggcctcact tctgctgttt cgcaggtctt tgagtttctg ctgatactcc gccttgatgg
    20341 ccctgcactc ttcgacagtc cagcgatggc ggttatggtt tgattcgatt tcgtctactg
    20401 cttcctgccc gatgcggcta atcagttcga cgcgatacgg aacgagattt ccgcttttgt
    20461 gctggttgca caccacgcat tgcttgtgaa tattgcgttc atcaaatcgg agttgaggtg
    20521 ccgcagcagt tgtccggtaa tgtccggcat cccactgagc agacgtgagc gttccgcaag
    20581 agatacatgg taagtcgcgg tctctttctc tgatgaaggc gtttacggct tgttgggctt
    20641 gtttaatcca gtaactgcgg ggctttaagg cgagttttct aatcttaagt ttatctttct
    20701 gtttctgctc ctctcgtcgt cgtttcttct ctgctgcttt ttccgctttt tcgcgttctt
    20761 tacttcgtcg ttcgagtgct atcttggttc cacactctgg agagcaccac cactgattag
    20821 cgaatgcagg gtgaaaccat tcccgacatt catcgttttt acatcgtctt cgcgctggtt
    20881 tagccatcat cttcttcctc gtgcatcgag ctattcgaat cgctcatcag ctctgcacag
    20941 cagtgctcac acacgtgaac ttccagcaca tgcagcttct gaccgcagtt agcgcacgtt
    21001 aaagcccgct cgacactttc ttgttcgtaa cttcgatttg ggtcaatcac cttgttttcc
    21061 tcgcacgttc tctaagccac cggatatcct acaggtgagc cgtgtagttg aaggttttta
    21121 cgtcagattc tttttggatt agcttgcgtt tatttctgga gcgcttcgtt ggaaggtatt
    21181 tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatg ccgccctcct
    21241 gacgccctgc ccgatcgcca tcaatgccgc tttggatacg gtagtaaaca tccgtcgagg
    21301 actgatgaac ggtcgccaaa tcagcagcat ggagcctttg ctgtttccct tcttctccag
    21361 ccctgtcgat ggttcgataa aattaatccg tccatcagtg ataatgcgaa cttcgtcgac
    21421 actctccaga gccttgctga accatccgac tgacatatcc tctggcacaa gcataactac
    21481 cgtctgtcgc tgttgtatgc actgctcagc ggctttttcc acccacggcc tgatattgct
    21541 gtacggtggg ttattccaga ttgcaccgtg gcttacccac tcagaattga gtgcgtcgtc
    21601 ggcctcagtt agccagtgag cacacagagc atttttgtcg ctcgctgccg aatccagcca
    21661 gaatccaaac tcaatatcca gtgcatcaaa aagccaaagc ggcgtttgcc agcagtctct
    21721 aacttctttt ggtgtttctg atttatgctt aatcattcaa ccctcacata ttgtccgtga
    21781 tatttgttga tagtcatgcc gccctccctt ttcgttgtga ccattcatac tctcgccggg
    21841 agtcatcact ccaccgcacg ttgcgctctg agccgaacca gaacatgatt tcgataagct
    21901 cagtcatgct ggcctttcgc attttgctgg tacgcacgcc aagcatgaca acgccaccgt
    21961 cgataccagg cgcacttctt tgctccattt ttttggtctt aagccacagg gcagtgaaca
    22021 gatctttcca gtcctccggc gcaagtctct ttccatgcca aagcacctga cgtgatacgt
    22081 cctgcaataa cgcccacata aggcggtttt gaggattgct ccgctttggt tctttaatgt
    22141 ggacttcgtg aggtgacttg tcgtcgatgg gaagtgagag tattgcgtct atggcattgt
    22201 ttctgattgc ttcgttgcga agcatgtata tttgcttcat cgttacctca actcacaaaa
    22261 cgccacgcca ttttataggc tccatccatc ccgttttctc tcaccaaaaa tatgcctgtg
    22321 ccattcttcc cgctgccgtc tccatagttc catcatgtct ggctggttct tttctcgcat
    22381 ttttcggatc tgctcaagga tgaactctat ttgctgctga tttgtcatgc tcactccttc
    22441 actttaaatt caggttccgg ataattctgt tgcgctgaaa ctcattgttg agtctgaaca
    22501 accgtcgaag aacacggtca cgcggatagc gtcgtgcggc aggtgaatgc tcatacaact
    22561 catcaagcgg caaactggac gatgaacgat accgatacca acgcaccaac tcttcacgaa
    22621 aattagccct gacaagctca gctatcgtac tcatttctta aaacctccaa ttcctctccc
    22681 ccaaataaaa aggcctgcga ttaccagcag gcctgttatt agctcagtga tgtagatggt
    22741 cattgcttca tctccctttc catttcatca atgtcaacgt catcaggaag atgggaacaa
    22801 tacgctgcta taccatgatg atttatctca taccctttaa acgttaccat ctggcgcgta
    22861 atctcaactt cgttcaggaa tccgccatcg cataactgcc tggctatttt cgatttggtc
    22921 tggattattg gtagtgcctg ttctttcaaa gcgcatgata tttgtgcatc ccatgccttt
    22981 tcgagaatgg ctaattgttt tttattcatt taatactccg tcacgttttc ctgtcgccac
    23041 gcctcgtcat attccgattt cggcatattg gcgatgtagc tatatggcga tcctgattca
    23101 agttgcagga actggtgcga ttgctcgtca aggaacaacg ggacaccacc ttcccaacct
    23161 tcgccgttac gttgtttttc aagcatcaaa acagatgccg gagatgccag tagctgttcg
    23221 tccttctctg acatcttttc accactctga actctctgta acgctctctc gcgagccttg
    23281 ttacgccaga tgatgaaaag gttgtctgtc aggtctgtta tcgctccaga gccttttacg
    23341 tccattttcc cggttggttt ttcttcgctg tctccttttc gcgagtgagt aacgagaatg
    23401 acgtgggagt ttgttttgtt tttgaagtcg caaatcgagt caacaaacgc cttctgcccg
    23461 ttatagtcat cgtcacctat gccgcatttc atcaggctgt cgatgatgaa taactggatg
    23521 ccgtatcggc ggcgagcgta gtcgaatatt tcgatcagcc tgtcggcttt cgccgttccg
    23581 gtcagaccaa acacccaaag tctttcgtca taaaatttaa atgcagagtc aatttccagc
    23641 actggcggca tcttgcagca cgtcgcctga cgggtaaggc gcttaaggag aataccaggc
    23701 ttcagctcaa gtgacgcgat gcacgtcttc acaccctgac gcattgcctc aagagccata
    23761 tgcccgacaa cctccgtttt tccgtgaccg ttcacaccat tgaccagcgt caactctgcc
    23821 tcacggaact ggaatttatc tgccagagat tcccacggtg gattaaacag atactgctgc
    23881 ttgccgtaga aagcgttgat agcgtcctgg taaaactctc gcgcgctgta gagttcttca
    23941 ggatcgaagt aggatgccgt gccgatgtac tgccagattt catcctcggt aacaccgttc
    24001 atcaggcatt cgttgatgtc tttgtacggc agagcaacaa gacggcaacg atgttcaccg
    24061 agtcggcttg cgatttccct tgcggcttca cgaccaacat catcaacgtc catcgagatg
    24121 aatatttcct caaacctgtc gaggttgtga tactcaaact caatccactg ttgcttagcg
    24181 cctttcccgc caccaaacgg cacggataac gccgagatgc cgtattgcgc atagctcata
    24241 caatcaattt cgccttcgca aagtacaacc gccctcacgc cagcgtccag agcctgccat
    24301 ccgaacagac aaggttcgca atcaccttct gccataatga ctttcttccc gtccgggcgc
    24361 tcagtgctga ttcgcttgac ctgcaacaac tcaccatcgc gtttgtacgg aatcaccaga
    24421 gcatccagtt cccgctctcc attccacacc ttgccgctga caacctcgta gcgctttacg
    24481 acttctggcg atatgccacg cgattgcagg tactcaagat gggattctgt tctggtaacg
    24541 tagcgggcga ttttcttgcg atcaggtctg gagaatttct tctcacgttt ggcatcgaaa
    24601 tggtgatcgt catccttgat tccgagaaag gctttcgctt cctgcatagc ctgatgcagg
    24661 ttaattccac gacatgccat ccacaaatca agcatgtcac cgccgtctcc ctcagcgaaa
    24721 tcagcccatt ttttcttgcc gctaaggttg accttaaggc tgtttccctt gtcaccgttg
    24781 acgttaccgg caacccactc atgcccctct ttcttgccgt ttggcaacag gtgcggagcc
    24841 accctgtcaa cctgcgccca aagcaggtca ctgagttcac ttggcgtcat gattccctca
    24901 gattgagatt tttaaaccag aaatcgacaa acgaaatact taaccagccg tggttataac
    24961 cagcgaccag tagcgatttg atttttgatt tcatggttca cctgtcgaaa aacacgtagc
    25021 cagttttcga tacggtgatt gcggatgatg gtttggattg tggttgaata gtttctggct
    25081 tctcgtcgtt ccagcgctga ccgttcaggt agctcgatgg taacaacctg tcgaatccga
    25141 actgcttacc attcctgcat gcgatgtctt ctgccagcat cgtggcaaac tcgcttgccg
    25201 tacccctggt agttttacgc cattccctga actgtgttct gaatgccgaa gctgcgtttt
    25261 tcttcccggc tttccgcatg cctgcacacc agaatatttc ctcgaatgcc ttgtcggttt
    25321 cttcgtgacg gtcaggtgat ttttcacact ccgtccgaac actttcggac atagtgtttt
    25381 tattatttct tttttctttt gtaatagttt cttttgtgtg tccctgtttt ggtgacagcg
    25441 ctgtcaccgt tttggtgaca ctttttgtca ccaatgcagt gacattatca ccagagtagt
    25501 gacacccttc gatttgccat tcctcgatgt tcttgttagg cccgatttgc tggccttcgc
    25561 gaaggataac cttcatcgcg ataagctcat tcttggcctt gtttaccttc tgtcttggca
    25621 gcctggtaat ttgagctaac tgactatcag agatgcgatc catcttttta ccgtagccgt
    25681 atgttttacg gcatatggcg tgggcaacct tgctctgatt tttcgttaaa tctgcgccga
    25741 taagctcttc atacagggca tttgcaagac gggtataacc atcttcaact tctgccacac
    25801 gacgctccac aggccgttgt gaaggcctta aatgtgttac ggttgcaaga ttactcatga
    25861 cctttctcct tctgcatcag cttcactttt tccaactcag cccggaatcg accaggctgc
    25921 ttgaagctgg acaggaagcg atcacgtagt atgtgtttgt gaattttgtc ctggtaagga
    25981 ctgagttgtt ttgtcataat tactcctgtg gattgatcca gtaatgacct cagaattcca
    26041 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatcgaag
    26101 caacttgtcg tgccaatcga gccatatcgt cgtcaacgac gccccattca agaacagcaa
    26161 gcagcattga gaactttgga atccaatccc tcttccacct gctgatctgc gacttatcaa
    26221 ctcccacagc ttccgctgtc ttctcagttc caagcattgc gattttgtta agcaacgcac
    26281 tctcgattct tagagcctcg ttgcgtttgt ttgcacgaac catatgtaag tatttcctta
    26341 acaaataaga agttatgcgc atcaacttat gcgcgttgta ttcccgcatt tcggcgggaa
    26401 tgaggaccat gactgttaaa gagcggtgtt acttatgctg cctgattcgg ttttggaaac
    26461 aggtgtggca aatcggggcg aatttcgtaa gccttgatct gccctccagt ggcgttaacg
    26521 atggcggtaa ctttctctgg agagaccaat ccgcctttca gccatttgtg tactgctggc
    26581 tgcgttacac cacacttgtc ggcaaggcgc ttttggctac cgacaatttt caaggctcgt
    26641 tgaattacta aattcatgag catacctctt gtggtcattg attataacca aagataactc
    26701 aagttataaa aaatagcaat aacctttgtt attttacttt ggataaccgt agttatagat
    26761 ttgtgggtat gaaaacattc gcagaaagac taaatgcagc catgagctca gctggggtat
    26821 cacaatcaca gcttgctgac atggttggaa tatctcagcc agccatacag aagatgtcgt
    26881 ccggtaaaac aaacggatct cgcaagatgg ttgaattagc caatgcttta aaagtgcgcc
    26941 ctgaatggct tagttctggt attggtgaaa tgagggatgg tgcacatgaa gaaccatcca
    27001 atgtccgtga gtcatcttta aaagctgtgg tatgggaaga cattaaaaga aacgatgacg
    27061 agtttgttgc gttgcctctt cttaacgttt cgctttcagc tggaagcggt agctgcgagc
    27121 tagaggaatc atcggagttc tctttggttt tcagaaagca ctatctgaaa aagatgggag
    27181 tatctgaaag atcagccaag ctagttaggg ttgtagggca aagcatggaa ccaacgcttc
    27241 acgatggcga tgttgttggt gttaacacgc aagataccac aatcagagat ggtaaaacct
    27301 acgctatttg ccagtctgat ttgttacgag taaaaacatt aatcgccacc cctacatcgg
    27361 tgataatcag atcaataaat cgcgaagagt acccggatga agtaatggat agagatgaat
    27421 ttcatgaaac cgtaaggatt attggcagag tattctggtc gtctcatagt tggtaaccga
    27481 taatcagaag aagacttacg gaagtgcgga gggataatgg aatttctgat agtttttgtt
    27541 gttgttttga tcatcattct ttttgttttg ctaagcatta gcaaaaagct atctcaaatg
    27601 atcgaacata gctctaatcg agcaaaagaa gaagagcatc taattgatat aaaagagatt
    27661 ctctctgata taaaaatcac attagatgaa ataaaataca caacagatct aattgaacag
    27721 tataaaatac caaccccaaa cgagagaaaa gcaatagatc aatatcgtat tgacttagaa
    27781 atcgacgaaa tgctaagcaa tagaaaggac taaaaacacc cggcctcagt gccgggtttt
    27841 tcttaccctg ccgttcacca cccaatcaac catcctcagc atagacaagc atcaagccaa
    27901 aggtaacacc ttcaccccgc acgcaagccc tcaaacacca atcaatcagc aacatttaca
    27961 aaaataaaat acctttgtta tccatcactt ataacttatt taccacaaaa tataaattag
    28021 gttattgacc acacctataa cctaagttat ctttaagcca tcagcaggac gctggaagcc
    28081 aaacggaaca gattggcagg ctctttaaca ttgatgggat tgtcccgccg aaatgcggga
    28141 accaaagagt agttggcttt ggggtgacgt gaagtgcagc tgcacgacgg caaccggaag
    28201 ataagcaccc ggcgcgtcac cgccaaagtc aatcatcgga ggtcaacatg acagtagtca
    28261 ttacatatct ggctgacgat aacgccagaa atcgccgcag agcacgcaga caggctcaac
    28321 gtgaacaggc aatgcaagaa cagcgattgg cacgaaaaat tgcgctaaag ctctctggtt
    28381 gcgtcagagc agataaagca gcatcactcg gaagccttcg ctgcaagaag gcagaagaag
    28441 tcgagcgtaa acagaaccgt atttactacc gcaagccacg tagtgaaatg ggtgtgacat
    28501 gtgttggtcg ccagaaaatg aaattaggca gcaaaccact gtatgagggg tgagatatga
    28561 gctaccaaat tcaatataga agttgcggta gatgggtttg cggaaactct ttcaaaacaa
    28621 aagaacaggc tgagcaatac gcaaaacaaa caggtcagga ttatcaaatt gtaaagacaa
    28681 ggagataaca tgccaggatt tacaagtaaa ttcataccaa gaagagaatt caagcataat
    28741 ccagaaagac taaatgttat ttcttccggt ggtgggactc aaagtaacgc aataatctgc
    28801 ttagtacatg ctggcgttct tccaaagcct gacattatcg ttatgtcaga tacagaacgt
    28861 gaggcaagta atgtatttga atatcagaaa aaatatatag ctcctatttg tgaagatatg
    28921 ggtgttgatt acctgattgt tcctaaaagt aaatttgcta cctacgacat tgatgggcct
    28981 gatccagacg ttcctctacc tggatatttc acagaatacg agggaaggga ttcaaaagga
    29041 atgtgcgctg gaaagcaacc agcattttgt tctataaagt ggaaaactga agttattcag
    29101 agatttttaa atgaaagata tggagagaaa tatcttacag caagaggagt ggatagttgg
    29161 atggggataa gcattgaaga ggctaaacga agagcgaaat acccaactgg caagtggcag
    29221 aggcgctatc cacttattga aatgctaatg actaaacaga tggcaattca atgtgtagag
    29281 gattatggtc ttcctacacc acctgcctcg ctttgctgga tgtgccctaa cagagatgat
    29341 gatctgtggc tttatatgaa aaataacgtc ccatctgatt ttgaaagagc atgtcagcat
    29401 gaaaaagaga tacaaatgaa gtggcctcat ttgt
//
LOCUS       DBBGLQ010000052        28775 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.052, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000052 DBBGLQ010000000
VERSION     DBBGLQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.052"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..729)
                     /locus_tag="ACIYSB_003116"
     CDS             complement(<1..729)
                     /locus_tag="ACIYSB_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGC5430108.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWIS"
     gene            complement(740..1741)
                     /gene="ltaE"
                     /locus_tag="ACIYSB_003117"
     CDS             complement(740..1741)
                     /gene="ltaE"
                     /locus_tag="ACIYSB_003117"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGC5430109.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1778..3496)
                     /gene="poxB"
                     /locus_tag="ACIYSB_003118"
     CDS             complement(1778..3496)
                     /gene="poxB"
                     /locus_tag="ACIYSB_003118"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGC5430110.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATMHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3629..4597)
                     /gene="hcr"
                     /locus_tag="ACIYSB_003119"
     CDS             complement(3629..4597)
                     /gene="hcr"
                     /locus_tag="ACIYSB_003119"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGC5430111.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIVGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4609..6261)
                     /gene="hcp"
                     /locus_tag="ACIYSB_003120"
                     /gene_synonym="priS"
     CDS             complement(4609..6261)
                     /gene="hcp"
                     /locus_tag="ACIYSB_003120"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGC5430112.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6405..7304)
                     /gene="lysO"
                     /locus_tag="ACIYSB_003121"
     CDS             complement(6405..7304)
                     /gene="lysO"
                     /locus_tag="ACIYSB_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGC5430113.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7799..8494)
                     /gene="aqpZ"
                     /locus_tag="ACIYSB_003122"
     CDS             complement(7799..8494)
                     /gene="aqpZ"
                     /locus_tag="ACIYSB_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGC5430114.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8920..10578
                     /gene="ybjD"
                     /locus_tag="ACIYSB_003123"
     CDS             8920..10578
                     /gene="ybjD"
                     /locus_tag="ACIYSB_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGC5430115.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPALRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRMAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10575..11531)
                     /gene="ybjX"
                     /locus_tag="ACIYSB_003124"
     CDS             complement(10575..11531)
                     /gene="ybjX"
                     /locus_tag="ACIYSB_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGC5430116.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRECMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11682..12797
                     /gene="macA"
                     /locus_tag="ACIYSB_003125"
     CDS             11682..12797
                     /gene="macA"
                     /locus_tag="ACIYSB_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGC5430117.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTKAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12794..14740
                     /gene="macB"
                     /locus_tag="ACIYSB_003126"
     CDS             12794..14740
                     /gene="macB"
                     /locus_tag="ACIYSB_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGC5430118.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVTGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLAQLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14813..15037)
                     /gene="cspD"
                     /locus_tag="ACIYSB_003127"
     CDS             complement(14813..15037)
                     /gene="cspD"
                     /locus_tag="ACIYSB_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGC5430119.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15187..15261
                     /gene="yljB"
                     /locus_tag="ACIYSB_003128"
     CDS             15187..15261
                     /gene="yljB"
                     /locus_tag="ACIYSB_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGC5430120.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            15442..16680
                     /locus_tag="ACIYSB_003129"
     CDS             15442..16680
                     /locus_tag="ACIYSB_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5430121.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHELNHTVQE
                     ALAPVTVKDALTYWLESYAKEKRTDHESLKSRINKHIISQIGAMPLDKCELRHWLACF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAVSNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIFPPYYSALIRLLIVFGCRTTELRRSEVREWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGELKGQSSVAEYGRTAHRRINQAPWSL
                     HDIRHTFTTMLNDLGVDPHIVEQLTAHQMPGMQRVYNHSRYLDAKRDALNLWVDRLEL
                     LQNNDEKIVVMTPRIYPQNS"
     gene            17100..17309
                     /locus_tag="ACIYSB_003130"
     CDS             17100..17309
                     /locus_tag="ACIYSB_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5430122.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGSFPSRRNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAA"
     gene            complement(17448..17639)
                     /locus_tag="ACIYSB_003131"
     CDS             complement(17448..17639)
                     /locus_tag="ACIYSB_003131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430123.1"
                     /translation="MNNQELQNKALSIIMRQREKTRREINKALTRTENLKVPDKFALD
                     PFVEKYPRRLKANKNRPPG"
     gene            complement(17697..17876)
                     /locus_tag="ACIYSB_003132"
     CDS             complement(17697..17876)
                     /locus_tag="ACIYSB_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGC5430124.1"
                     /translation="MATKAVNAKSQTVAARVPHEVMNNVEAVKMPGESTGQFVTAALK
                     REVEYRQRRKAKESE"
     gene            18076..19308
                     /locus_tag="ACIYSB_003133"
     CDS             18076..19308
                     /locus_tag="ACIYSB_003133"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGC5430125.1"
                     /translation="MLNHTTHPQGRDPHNLNKYIWRFIALSTAQPRVITIGATSEQEA
                     RQQSPTGWVMVLASRFHHRCLFFVCLSSGYGVVPFQRMRGRSLMKRSSGKLSDGAGGR
                     TRRSRLRWRTTLASVISPDSFIFIKSYLRAHLLWRFLFLMKTYSGSGVSYFKIWFNFS
                     CLALMTARNNICRCFVFTSRTVGDNMDLFMVLTCNALRRDASYHGGGYRFVRIRPLMK
                     SSSGKCSGRGNSGGTGLLLMRSRSLAARAAFSFCRSTSNGTPLSFMVPPVYPLLTDGY
                     RCIFRTNYLINTSDHIIREPEYTGAIIPHLLAYAQLTADMALNEAKAILIADCEYGVM
                     RDDRFNALKQEFDGTPEDTDIALLCVADMVKAACFLLETAEHSGTGSDILNIASDYAE
                     YVAEARYRRKFQEVVSHE"
     gene            19301..19500
                     /locus_tag="ACIYSB_003134"
                     /pseudo
     CDS             19301..19500
                     /locus_tag="ACIYSB_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001474135.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19505..19804
                     /locus_tag="ACIYSB_003135"
     CDS             19505..19804
                     /locus_tag="ACIYSB_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGC5430126.1"
                     /translation="MKKKNSGFTASGLTRPEIRPGDIFRDNYGGTVTIKGVAERRITY
                     RHEGYEYDCVMPVYQFVRDFSLVQAVPRNVPTSREKARANIQKMKTMINGFRGKK"
     gene            19801..21549
                     /locus_tag="ACIYSB_003136"
     CDS             19801..21549
                     /locus_tag="ACIYSB_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HGC5430127.1"
                     /translation="MKLAPNVKLQSRGIKHKGTEVIIFAGSDAWAHAKQWQEHDARMA
                     GDNEPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGYLAPVMIKAIGQKLAAAGVQDA
                     NFYPEGMHGQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQMLAGRFDG
                     VCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPNFH
                     KWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPERIAAEIP
                     VIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVAEFSKAMKPAAKVYGHEYITRRIKG
                     VTQTNVTTTDDCDAFL"
     gene            21836..22093
                     /locus_tag="ACIYSB_003137"
     CDS             21836..22093
                     /locus_tag="ACIYSB_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430128.1"
                     /translation="MHTSGKLNNQKKPHNRAIDLTEHWLRVAIKIIDRSAGEGYAKAH
                     PELISAFMTTVAANFATLTEREIAGAEQVTTINVKTGEHTA"
     gene            22090..22500
                     /locus_tag="ACIYSB_003138"
     CDS             22090..22500
                     /locus_tag="ACIYSB_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGC5430129.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMAMASMAVPLPCSQADDGT
                     ATMWLSVLAFGRQAEALAKHRKGELLSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTVRPGGKTRKTTGAQGNKPPAGGDDPYGDDIPF"
     gene            22518..22838
                     /locus_tag="ACIYSB_003139"
     CDS             22518..22838
                     /locus_tag="ACIYSB_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGC5430130.1"
                     /translation="MNNSTPKNRTISDATAEELESRGLWRRAARRWQDVMITLQEDRH
                     RELAAMRARECIRKAKRPIPENRYDVHAVRRAADRTIQQMGLPSLTDEIWRDYPDNVN
                     DDGY"
     gene            22918..23502
                     /locus_tag="ACIYSB_003140"
     CDS             22918..23502
                     /locus_tag="ACIYSB_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGC5430131.1"
                     /translation="MTKLTINRKPKGIYGTPQKKTQEAEQPDKTTPAQKQSREQKTQQ
                     KTAAKRKSEKSRKKNIRRVERLAALWPELFSQESPRPLKVGIFDDLMEDITARGISLG
                     QGALRATLAYYVSSVRYYRSLMAGGVRYDLKGHPCGEVTQEHQLNAGKRLDEMKKKQQ
                     ERRNMKAENKHNGKPPAGHNIILKNASSSGVRKA"
     gene            23731..24273
                     /locus_tag="ACIYSB_003141"
     CDS             23731..24273
                     /locus_tag="ACIYSB_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGC5430132.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVIPVERKGRETFYDVASV
                     IDNRVNNAISQITNDKGEIDDDELLRVRIRLLTAQAEAQELKNERDRGDVIDTEFCMY
                     ALSKLASQISSIMDSLPLTMQRSFPQMTPAMLDGLKKEVVRACNACTKLDENIPRMLS
                     DYLMETTGNVPDKFQPDKDK"
     gene            24525..24809
                     /locus_tag="ACIYSB_003142"
     CDS             24525..24809
                     /locus_tag="ACIYSB_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430133.1"
                     /translation="MTETEALGIIRSITGISQQAGEQEATQPDSVIAENYACVVAEVM
                     RRDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKKPERLRR"
     gene            25833..26153
                     /gene="clpS"
                     /locus_tag="ACIYSB_003143"
     CDS             25833..26153
                     /gene="clpS"
                     /locus_tag="ACIYSB_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGC5430134.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            26184..28460
                     /gene="clpA"
                     /locus_tag="ACIYSB_003144"
     CDS             26184..28460
                     /gene="clpA"
                     /locus_tag="ACIYSB_003144"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGC5430135.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(28539..>28775)
                     /locus_tag="ACIYSB_003145"
     CDS             complement(28539..>28775)
                     /locus_tag="ACIYSB_003145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430136.1"
                     /translation="RAFFSHFICLYGSEHGGRVEYVTCEFLSCLYGSELKYYTFSPLL
                     IKQNHPILLQHPFLSLPSRSTNYQQLTIIIKKRV"
BASE COUNT         7406 a         6937 c         7749 g         6683 t
ORIGIN      
        1 cgaaatccag cgggtgggga gggggatggg gatcaaccag cggcgcttac cgctcaccgc
       61 cataaaatgt tcaaactgtt gctgataact gagcacctct ggtccggcgg cttcgaagat
      121 gcggtgttcg ctggccggat gatctaacaa cgcaaccaga tagtgcagca agttttccag
      181 cgcaatgggc gtggtgcgtg aacgaaccca gcgcggaggc gttaagactg gcaggttgta
      241 gaccatatcg cgcatgactt cgaacgccgc ggaacctgcg ccaacgataa ttccggcccg
      301 cagttcggtc acgggtacat tcgcttcacg cagaatgtcc gccgtagcct gacgagcacg
      361 cagatgatcc gactgctcat gtggcggggc ctgcaacgaa ctaagaaaga ttaattgctt
      421 aactggtact tcacgtagcg catcgcggac gttgagagcc acctggcgct cctgagcgat
      481 aaaatcgccg ccttcgccca tgctgtgcac cagaaaatag accgtatcga tatcctgcaa
      541 cagggccggt aggttatccg gccagctgag atcgacttta tggcaactga cgtttgccag
      601 ttgcagcttt gcaagcctgt cgacatgacg tgccgccgcc aggatctgat gcccttgctg
      661 gctgagtgtg cgcaccagat gctgaccaat gtagccactg gcaccgagaa ctaaaatgcg
      721 ttgcggcacg tctctctcct taacgcgcca ggaatgcacg ccagtgggcg gcgacttccg
      781 ccagttgttc gcgcgagacg tcaagatgcg tcaccaggcg gacaatcggc gaggcgttaa
      841 tcagcacgtt tctcgctttc atgtattcgc ctaacgcggc agcattttct tccccgacgc
      901 gaacaaacag catattggtg tcctgacgca tcacatccgc gcctgcttca cgcagctgct
      961 ccgccatcca ggcagcgttg tcgtggtctt cctgcaagcg cgcaacgtta tttttcaggg
     1021 catatatccc ggcggcagcc agaatgccgg actggcgcat cccgccacct gtcattttcc
     1081 gccagcgaat ggcacgttta atgtaatcac gattaccgac gagtaatgaa ccgactggcg
     1141 tcccaagacc tttcgacagg caaatggtga acgaatcaca atattgcgtg atctctttca
     1201 gttcgcagcc gtaagccacc acggcattaa agatgcgcgc accgtcaaca tgcagcgcca
     1261 gattgcgctc gcgggtaaat tcccatgctt ctttcaggta ttcccgcggc aacactttgc
     1321 cgttgtgggt gttttccaga ctgagtaatt tggtgcgggc gaaatggata tcgtcgggtt
     1381 tgattttcat cgccacttta tccagcggta gcgtgccgtc ggcagccgcg tctatgggtt
     1441 gcggttgaat actgcccagc accgccgcgc caccggcttc aaacagatag ttatgcgcgg
     1501 cctgaccgac aatatactct tcgccgcgtt cgcagtgact gagcagagcg accaggttgg
     1561 cctgagtgcc ggtaggcaga aaaatggcgg cttctttacc ggaaagctct gctgcgtagt
     1621 cctgcagagc attaacggta gggtcgtctc cgtaaacgtc gtccccaacc ggggcggcca
     1681 tcatcgcttc gagcatggcg cggcttggtc gggtaacggt atcactgcgt aaatcaatca
     1741 tggcatgtcc ttattatgac gggaaatgcc acccttttta ccttagccag tttgttttcg
     1801 ccagttcgat cacttcatca ccgcgtccgc tgatgattgc gcgcagcata tacaggctga
     1861 aacctttggc ctgttcgagt ttgatctgcg gtggaatggc taactcttct ttggcgacca
     1921 ccacatccac caacaccgga ccgtcgatgg agaaggcgcg ttgcagggct tcatcaactt
     1981 cagacgcttt ttctacacgg atacccgtaa tgccgcacgc ttcggcaatg cgggcaaagt
     2041 ttgtgtcgtg tagttcggtg ccgtcagtca aatagccacc agctttcatc tccatcgcca
     2101 caaagcccag cacgctgttg ttaaagacga caattttcac tggcagtttc atctgcacta
     2161 ctgagaggaa atcgcccatc aacatgctaa aaccgccatc gccgcacatg gcgaccacct
     2221 gacgttctgg ctctgtcgcc tgcgcaccca gcgcctgcgg catggcgtta gccatcgaac
     2281 cgtggttaaa cgaacctaac aggcgacgct tgccgttcat ttttagataa cgtgccgccc
     2341 acaccgttgg cgtaccaacg tcacaggtga aaatagcgtc atcggcggca aaatgactaa
     2401 tttgctgcgc cagatattgc gggtgaatgg ctttctcgct cggtttagct aaatcgtcca
     2461 gccctttgcg ggcgtcgcgg taatcttcca gcgctttatc cagaaacttg cgatcggctt
     2521 tttcttccac caatggaagc aatgcacgca gagtcgactt gatatcgccg accagtgcca
     2581 tatccacctt gctgtgagcg ccgatgctgg ctgggttgat atcaatctga atgattttgg
     2641 catcggtcgg gtagaaggcg cggtagggaa attgcgtgcc gagtagcact aacgtgtcgg
     2701 cgttcatcat ggtatggaaa cctgacgaga agccgattaa cccggtcatt ccaacatcat
     2761 acggattatc gtattcgaca tgttctttac cgcgcagggc atgaacaata ggcgctttaa
     2821 ttttcccggc aaactcaact aactctttat gcgcccccgc gcagccgctg ccacacatca
     2881 gggcgatatt gctggaataa cgcagcagtt gcgccagttt gcgtaactct tcttcttccg
     2941 gcgtcacgac tggttgtggc gcatgatacc agtgcatggt tgccccttct ggcgcaggtt
     3001 ttaacgccac gtcgcctggt aacacgacaa ccgaaacgcc acggttaagc accgctttgc
     3061 gcatggcaat cgccagtact tgtgggatct gctccgggct ggaaaccagc tcgcaatagt
     3121 gactacattc gcggaatagc tcttgtgggt gggtttcctg gaaatagccg ctgccaattt
     3181 cgctggaggg aatatgagcg gcaatcgcca gtaccggaac gtgattgcgg tggcaatcga
     3241 acaggccgtt gattaagtgc aggttgccgg ggccgcacga tccggcgcag accgccagtt
     3301 ctccgctaag ttgtgcttca gcgccagcgg caaaggccgc cacttcttcg tggcgggtgg
     3361 acatccactc gatggtgccc atgcgattaa gactgtcact aagaccgttc agagagtcgc
     3421 ctgtgactcc ccagatgcgt ttcacccctg ccgattcgag tgttttggcg atataagctg
     3481 caaccgtttg tttcatggtt ctccatctcc tgaatgtgat aacggtaaca agtttagttc
     3541 atctgacgga gggggaaggg atgggagaga aaggaggcac taacggttaa atagcccgat
     3601 gaaaggaata tcatcgggca taaggcgatt atgcgagaac caaatccccc tgcggatggc
     3661 aggagcaggc cagtacgtaa ccttcagcga tttcggcgtc ggtcagcgtc attgtgctgc
     3721 tcaccgtata ttcaccggaa accacttttg tcttacagca gccgcaaaca cccgcacggc
     3781 aggcagcgac aaccggaacg ttattgcttt ccagcgcctc cagtagcgtg gtgccaaccg
     3841 gggcgtaaaa ttctcgtgcc ggttgcagtt tggtgaattt cagaccgctg gtcgccgctt
     3901 ctgctactgg ggtgaagaat ttctctttaa agaaacgcgt cacgccgagc gctttcactt
     3961 cctgctctac ccaatccata tacggagccg ggccgcaggt catcacggta cgtgaagcta
     4021 agtcaggtac acctgccagc agttcgcgag tgagacgacc gacgataaag ccttcggtaa
     4081 cgttattttc tgccaccagc gttaccggat agttacgcca ctcatcggcg aaaataacat
     4141 cctgcggcgt acgcacgttg tagatcaccc gcacatcggc ctgtggacgg ttcttcgcaa
     4201 gccagcgacg catcgacata atcggcgtga cgccgcagcc tgccgccagc aacaggaatt
     4261 tatcttctgc tttatcgtcg caggtaaatt cccccatcgc gtccgaaagc cagagataat
     4321 caccgcgttt tacatcgcgc gtcagccact gggagccgac accgtcatca atccgccgca
     4381 cggtcagggt gatatattca ctcacgcctg gcgtggagga aatggtgtaa gcacgcagcg
     4441 tttccgctga gtttcgcacg ctgaccagtg catattgccc ggcgcgatat gggtagtaat
     4501 cgtggcaaat cagggaaatc gtccacacat ccggcgtttc ttgcgtaatg tgatgaacct
     4561 gcatccgcca cgggcattga ttcgttggca tcgtcatcga caaactcctt acgcgctcaa
     4621 cagttgcttc atgtcttctt caacagtggt gatagaacgc aggccgaatt tctcgttcag
     4681 caccgccagc aggtctggtg tcaggaaacc aggtgcagtc gggccggtga cgatattttt
     4741 cacgcccaga gaaagcagcg tcagcagaat gacgatcgct ttctgttcaa accaggagag
     4801 caccagcgac agcggcagat cgttgacacc gcagcccagt ttctctgcca gagtgacagc
     4861 cagaataatc gctgagtaag catcgttaca ttgacctgca tctaccagac gcggcagacc
     4921 ttcgatatcg ccaaactcaa gtttgttaaa gcgatattta ccacaggcga gggtcaggat
     4981 caggcagtca tccggcacgc tggtggcgaa atcggtgaag tagtggcgct cgccgcgtgc
     5041 gccgtcacag ccaccaagca ggaagatatg acgcagtttt tcacggctca ccagatcaat
     5101 cagcgtatca gcagcgccaa gcagcgtctg gcgaccaaaa cccacggtga taaggtgcgg
     5161 aatttcgctg tacgggaagc ctgccatctg ttgcgcctgg gtgataaccg cagagaaatc
     5221 atcaccgtcc agatgacgca cgccaggcca gccaacaatg ctgcgggtcc agatacgatc
     5281 gtcataagcg cctacggttg ggtcgatgat gcagttcgag gtcatcacga tggggccagg
     5341 gaaacgagcg aactccactt gctgattctg ccagccgctg ccgtagttac cgaccagatg
     5401 cttgaattta cgcagctccg ggtagccatg cgcaggcagc atttcgccgt gggtgtagac
     5461 attaacgccc gtgccttcgg tctgttccag caggttgtag agatctttga gatcgtgacc
     5521 ggaaatcaga atgcatttac ccgccgtcgc tttgacgttg acctgggttg gcgtcgggtg
     5581 accgtattta ccggtttcgc ctgcatccag aatgctcatc actttgaagt tcatctggcc
     5641 gatttccatt gaacactcaa gaagcgcgtt catatcggca ggccaggtcc ccagccacgc
     5701 catgatttta tggtactggg cataaatatc gttgtcgtat tgaccgagaa catgcgcgtg
     5761 ttccatatag gccgccgcac ctttcaggcc atacaggcac agcagacgca ggccgagaat
     5821 gttttcgcca atcgccgctt tatctttgtt aggggtaaat tctgctgcct gacgttgcag
     5881 ctcgccgaga tcgtcgctca ccagttgcag gtcagccatc gggttatcga cgcgcgcgtt
     5941 ggcatctaca gccaggcatt gcgctttcag cgcctcgcgc agggcaatcg cttcacgagc
     6001 gtagccgaca atacgcggag aatcgaagtt aacgttggtc agggttgaga aaaaggcacg
     6061 tggcgcgaag ctgtcaacat cgtggttgat gatgccgtat tcacgcgctt ttaccgccca
     6121 ggcagaaagc ccttgcagcg ccgcgatgag taaatcctga aggtcagaag tttccgccgt
     6181 tttaccacac atcccctgcg cgtatgagca gccgtttcct gccggagtac ggatagtttg
     6241 ttcacattgc acacaaaaca tgatcacacc ttttaaagtt atatttaata tacatgttta
     6301 aggttaagac gcttaacgcg gggataaaag ggatttttca tgcaacttta agggagattg
     6361 atttagcgca attttggcgg cagggatcta ccgccagaga ggtattacgc agagaaaaag
     6421 gcgatgagga tcggcactaa caggctaaga ataaaaccgt gaacaattgc cgccgggacc
     6481 atatccagcc cgccagtacg ttgaagaacg ggcagggtga aatccattga tgtggcaccg
     6541 cataagccca gtgcagtaga gcggctgcgg cgaatcagcc cagggatcaa cataatagca
     6601 atcagttcac gggccagatc attaaaaaac gccgcgctcc cgattaccgg accaaaagat
     6661 tcggtcaata aaataccgga aagagaatac cagccgaaac cggaggccat tgccagcgcg
     6721 gtattgatgg ggagatcaag aataaaggcg ttaattaaac caccaattaa tgaactgaca
     6781 accaccacca cggcgacaat cattccccgg cgattaagga caatctgctt taaggtcatg
     6841 ccattattgc gcaactgaat accaacgagg aaaagtagca aaattaacgt gtattcactg
     6901 gcttcggtcg cgtgttgtaa gaaagccagt ccacttagac caatggcaaa accaatcact
     6961 actacgccgc acagttttag cgactccagc gccatcgcaa tacgcgacgg gagtttttct
     7021 tgctgatggt ggttgcgcca cggcaggcct cgctccagcc acatcagggc ggcaatatta
     7081 cacagtaaaa taacggtaat actgacggca gaataatgca gaatcgccaa caggttactg
     7141 gcgaggttat cgagaaacgc cagactgata cccataaaaa agagaataag gtaaaccatc
     7201 cagcttaata gctgattaat aacttttaac gcagcttgtt ggcgaagcgg aatgaggtaa
     7261 cccacaatca ggggaaccag aatgattaac agcccagaaa acatgaaaac ccagtccttg
     7321 caaagatgaa gtcgaaatgc gcgatgacac actactgaaa gcggaaggac gagtaaagtt
     7381 gcaattaaaa ggaaatgtta tgcataagga gcagtagagt attcgttttc atttaaagat
     7441 attcttgcgc tttaattaca aactgcaccg atgttggtgg cgtcaaaatc gccgaggcgt
     7501 tccctgaagg ccggggcagc ccacatggat gtgggctgag ggcgcgtttt acagggatgt
     7561 tacctcgcgc ccgacccggt agccgtaagg gataagtcga gggcaccgcg cagcggcgat
     7621 tttgttcgcc agagcccggg ggtgcagggg gcggcggcga ttggccgccc cctgcgcgct
     7681 ccttgcgcca gtggcaatat gttgcttagc tcatgaaagg agcgcaacaa gatgatgaat
     7741 caacatataa caacatctta aaaaaaggcc tgacattacg ccaggccttc tgcgttaatt
     7801 aatcacgctt ttccagcagg gtccggtaaa tcagaccacc gataatgccg ccgacaattg
     7861 gcaccaccca gaagaaccac agttgttcta atgcccagcc gccctggaag atagcaaccg
     7921 cggtgctgcg cgccgggtta acagaagtgt tagtcaccgg aatactaatt aagtgaatca
     7981 gggttaaggc cagaccaata gcgatcggcg caaaacctgc cggcgcgaat ttgtcggttg
     8041 cgccgtggat caccaacagg aaacctgcac tcaataccag ttcaactacc agcgcggaaa
     8101 gcatggaata accgcctggt gaatgctcgc cataaccgtt agaagcaaaa ccgctggctg
     8161 ccgcgtcaaa acccgtttta ccactggcaa ttaaatacag cagcgccgct gcaacaatac
     8221 cgccgacaac ctgggcaatt acgtagccaa cgacttcttt tgccggaaaa cgtccgccag
     8281 cccataaacc aatagtgacc gccgggttaa aatgaccacc agaaatatga ccaacagcaa
     8341 aggccatcgt cagaacggtc agaccgaacg ccaacgccac gccggcaaaa ccaatgccta
     8401 attccgggaa gcctgcggcc agtacagcac taccacagcc accaaaaaca agccagaaag
     8461 taccaaaaca ttcagctgct aattttctga acatatccac ctcaattaaa aattgaccct
     8521 gtgaaaaata tggtcgtttt atagggtcgt cgtaaaaaag tgacgacgga aataatgcgc
     8581 ggctatttta aaaacgaagg cgagtcattc accagataaa taaatccagt aaatttgatt
     8641 tagggcaaca gcgggttgcc ccatatagtc atttgtctga ttgacagtgt agtgcacgca
     8701 aaagatttaa tcctttaggc gtaataaaaa ataatttatc atgctaatta tttgattttg
     8761 ttgtttttgc agacttatca gcaagaggga gtataacgcg attattcgct catttttcag
     8821 acatttgcca tgcttaaatg tgatgtcatc acgtattagc aaggcctttc ccgttatact
     8881 gccagcgtaa aggataagtc acatatttct ggaggggata tgattcttga gcgcgttgaa
     8941 attgtgggtt ttcgcggtat caaccgtttg tcgttgatgc tggaacaaaa caacgtcctg
     9001 attggggaga acgcgtgggg taaatccagc ttgctggacg ccttaactct gctgctatcg
     9061 ccagaatcag atctctacca ttttgagcgc gacgatttct ggttcccgcc gggagatatc
     9121 aacgggcgag aacatcatct gcatattatt ttgaccttcc gcgaatcgct gccaggccga
     9181 catcgggttc gccgttatcg gccgctggaa gcgtgctgga cgccatgcac cgatggctat
     9241 caccgtattt tttatcgtct ggaaggggag agtgcggaag acggcagcgt gatgacactg
     9301 cgcagttttc tcgataaaga cggacatccg attgatgtcg aggatattaa cgatcaggca
     9361 cgccatctgg tgcgtttaat gccggcgctg cgcttgcgtg atgcccgttt tatgcgccgt
     9421 attcgtaacg gcacggtgcc aaatgtccct aatgtggaag tcaccgcgcg ccagctcgat
     9481 ttcctcgccc gtgagttatc ctcacatccg caaaatctct ctgatgggca gattcgtcag
     9541 ggactttccg caatggtaca gctgcttgag cattatttct ctgagcaggg ggccggacag
     9601 gcgcgatatc gtttaatgcg gcggcgagcc agcaatgagc aacgaagctg gcgctatctg
     9661 gatatcatca accggatgat tgaccgacct ggtgggcgct cgtatcgggt tattttgctc
     9721 ggcctatttg ctactttgtt gcaggcaaaa ggcacattgc gactggataa agacgcccgt
     9781 ccattgttgc tgatcgaaga tccagaaacc cgtttacacc ccattatgct ttcagttgcc
     9841 tggcatctgt tgaatcttct gccattgcag cgcattgcca ccaccaactc gggtgagttg
     9901 ctttcgttaa cgccggtaga gcatgtttgc cgactggtac gtgagtcctc gcgcgttgcc
     9961 gcctggcgtc tggggccgag tggcttgagt accgaagata gccgacgcat atcctttcac
    10021 attcgtttta accgtccgtc atcgctgttt gcacgctgct ggttgctggt ggaaggggaa
    10081 acggaaacct gggttatcaa tgaactggcg cgtcagtgcg gacatcattt tgatgccgaa
    10141 gggatcaagg tcattgagtt tgcccagtcc gggctaaagc cactggttaa atttgcccgc
    10201 cgaatgggga ttgaatggca tgtactggtc gatggcgatg aagcagggaa gaaatatgcc
    10261 gctacggtac gcagcctgtt gaataacgat cgggaagccg aacgagaaca tttaacggcg
    10321 ttaccggcgc tggatatgga acattttatg tatcgccagg gattttccga tgtgttccac
    10381 cgcatggcgc aaatcccgga aaatgtaccg atgaatctac gcaaaattat ctcgaaagcg
    10441 atccatcgct cttccaaacc cgatcttgcc attgaagtgg caatggaggc aggacgtcgt
    10501 ggtgtggact ccgtaccgac gctgctgaaa aaaatgttct cacgcgtgct gtggctggcg
    10561 cgcggtcgcg cggattaacc gcgaaacatc gtggccattt gtggctgaat agcgtcgagc
    10621 atctcatagc gccgacggta ttcagcccgt tttttactgg cgatttcggc aatctctttt
    10681 cgtgctatct gtgctggaag gcggtaatgg cgttcagcat cacatacgcc gccaaccgat
    10741 tcccagaaag cgttgtaatc agcgtggatc ttgccttctt tatcgcgata acgcaggctg
    10801 cggtaaatat gcgtttcatt gctgacggca ataatctgct ctacctgcaa acgttgggca
    10861 aacagacagg ccgcttccat cacgaggcgt ttgggaaata gcccgtggca ggctttcgtc
    10921 gcattctgga tttcctgatg tggaatttcc cattttgcgc cttgcagtcc gccaataaac
    10981 atcgttcttt tcccctgata ttcacacagg gtaaacgtga tctctgccag aggaatacct
    11041 tcgctgttgc ggaacaggat tgtgctgtca ccttctttat ccattgagat catcatggtc
    11101 agctcaagcg tgaactgctc gccgtttttg ccttccagct tcgccagttg cagcccgggg
    11161 gtattcaaat ataagctgaa ttcttccgcc gacatacatt cgcggagtaa cgcataatgg
    11221 taacgtaacg cctccagcaa ttgcttacgg ctaagattcg ccgcaaggta agggcgatgc
    11281 agacgcacag gcagtcgcgg ctggcgcgtt aacaatacat tgagattagg ccagtgggaa
    11341 agttcgttca tccactcaac gcttaaacgc ggcataatca acgagcgcag caaaaatttc
    11401 tggcgaaaac tacggcgatg ccagaattta cccggccgac actgtccacg tgccagacta
    11461 agaaaaagtg acaggctgct gagagattca gatggcgtaa aggtccgttc agttagctgc
    11521 gacatattca tgaaatcaat ggttatacat ggcgtcgatt tcaccattgc gtatcttaac
    11581 caaacatcaa tagtgtgatt actaacgtaa attttagggt tttgttgata tttcgttgaa
    11641 gttaatgacc cggattggca tatggagtat tcagaaaatt tatgaaaaag cggaaaaccg
    11701 tgaagaagcg ttacgttatt gcgctggtga tagtcatcgc cggactgatt acgttatgga
    11761 gaattcttaa cgcacccgtg ccgacttatc agacgctgat tgtgcgcccc ggtgatttac
    11821 agcaaagcgt gctggcgacc ggaaagctgg acgcgctgcg taaggttgat gtgggcgcgc
    11881 aggtcagcgg tcagttgaaa actctgtcgg tggcgattgg cgataaagta aaaaaagacc
    11941 agcttttagg ggttattgat cctgaacagg ctgaaaacca gattaaggag gtcgaagcaa
    12001 cgctgatgga gctacgtgcg cagcggcagc aggcggaagc ggagctgaaa ctggcgcggg
    12061 tgacgtattc ccgtcagcaa cgtctggcac aaacgaaggc tgtttcacag caggatctcg
    12121 acaccgccgc gacggagatg gctgtgaaac aggcgcaaat tggcaccatt gacgcgcaaa
    12181 tcaagcgcaa tcaggcttct ctcgatacgg ctaaaaccaa tctcgattac actcgcatcg
    12241 ttgccccgat ggccggggaa gtcacgcaaa tcaccactct gcaaggccag acggtgattg
    12301 ccgcacaaca agcaccgaac attctgacgc tggcagatat gagcaccatg ctggtaaaag
    12361 cgcaggtttc tgaagcggat gtaatccacc tgaagccggg gcaaaaagcc tggtttacgg
    12421 tgcttggcga tccactgacg cgctacgagg ggcaaatcaa ggatgtacta ccgacgccgg
    12481 aaaaggttaa cgacgctatt ttctattacg cccgttttga agtccccaac cccaatggtt
    12541 tgctgcggct ggatatgact gcgcaagtgc atattcagct caccgatgtg aaaaatgtgc
    12601 tgacgatccc tctgtcggcg ttaggcgatc cggttggcga taatcgttat aaagtcaaat
    12661 tgttgcgtaa tggtgaaaca cgcgagcgtg aagtgacgat tggcgcacgt aacgataccg
    12721 atgttgagat tgtcaaaggg cttgaagcgg gcgatgaagt ggtgattggt gaggccaaac
    12781 caggagctgc acaatgacgc ctttgctcga attaaaggat attcgtcgca gctatcctgc
    12841 cggtgatgag caggttgagg tgctgaaggg catcagcctc gatatttatg cgggtgagat
    12901 ggtcgcgatt gttggcgctt cgggttccgg taaatcgacc ctgatgaata ttctcggctg
    12961 tctggataag gccaccagcg gcacctatcg cgtcgccggt caggatgttg ccacgctgga
    13021 cgccgatgcg ctggcgcaac tgcgccgcga gcatttcggc tttattttcc agcgttacca
    13081 tttgctttcg catttaaccg ccgagcagaa cgttgaagta cccgccgtct atgctggtct
    13141 tgagcggaaa cagcgactgc ttcgtgccca ggagttgctg caacggctgg ggctggaaga
    13201 ccgtacagag tattatccgg cacagctttc gggtggtcag caacagcgcg tcagcatcgc
    13261 gcgggcattg atgaacggtg gtcaggtaat tcttgccgat gaaccaaccg gcgcactgga
    13321 cagccattct ggcgaagagg tgatggcgat cctgcatcag ctgcgcgatc gtgggcatac
    13381 ggtgattatc gtcacccacg atccgcaggt cgctgctcag gccgagcggg tgatcgaaat
    13441 tcgcgacggc gaaattgtgc gcaatcctcc cgccattgaa aaagtgaatg ttactggcgg
    13501 gacggaacct gttgtcaaca cggtgtctgg ctggcggcag tttgtcagcg gttttaacga
    13561 ggcgctgacg atggcatggc gggcgctggc agcgaataaa atgcgtactt tactgaccat
    13621 gctggggatt attatcggta ttgcgtcggt ggtttccatt gtcgtggtgg gtgacgccgc
    13681 caaacaaatg gtgctggcgg atattcgttc tattggtacg aatactattg atgtctatcc
    13741 cgggaaagat tttggcgatg acgatccgca atatcagcag gcgctgaagt acgacgactt
    13801 aatcgccatc caaaaacaac cgtgggtcgc ctcagccaca cctgccgtct cgcaaaacct
    13861 gcgcctgcgt tataacaatg ttgatgttgc tgccagtgcc aatggcgtga gcggcgatta
    13921 ttttaatgtc tatggcatga ccttcagtga aggaaacacc tttaatcagg agcagctgaa
    13981 cggtcgtgcg caggtcgtgg ttctcgacag taatactcgc cgccagcttt tcccccataa
    14041 agcagatgtg gttggcgagg tgattctggt cggcaatatg cccgccagag tcattggtgt
    14101 ggcggaagaa aaacagtcga tgtttggtag cagtaaagtg ctgcgtgtct ggctacctta
    14161 cagcacgatg tccgggcgag ttatgggcca gtcgtggctt aactccatta ctgtcagggt
    14221 gaaagaagga tttgacagcg ccgaggcgga acagcaactc acgcgtttac tttcactgcg
    14281 ccacggaaag aaggatttct ttacctggaa catggacggc gtcttgaaaa ctgttgaaaa
    14341 gaccacacgt actttacaac tgtttctgac gctggtggcg gtgatttcgc tggtggtggg
    14401 cggtattggt gtaatgaata ttatgctggt gtcagtgacc gagcggacgc gggaaattgg
    14461 cattcgcatg gctgtaggtg cgcgagcaag cgatgttttg caacagttcc tgatcgaagc
    14521 cgtactggtt tgcctggtcg gtggcgcgtt gggaataaca ctgtcactgt taattgcttt
    14581 caccttgcag cttttcttac ccggctggga gattggtttt tcaccgttgg cgcagctgct
    14641 ggcgtttctc tgctcgacgg tcaccgggat tttatttggc tggttacccg cacgaaatgc
    14701 ggcacgactg gatccagtag atgctctggc acgagagtaa tttttgagat aaaaatgcca
    14761 gccgatcggg ctggcatttt gcctttagga tgtacacaat gagacagaag agctatgcga
    14821 ctgccgcttc tacttcgacg ggcacaataa cactggcgtg attgcctttt ggcccctggt
    14881 ggacatcaaa ctgaacggat tgtccagctt ttagcgttct gtaaccatcc atctgaatgg
    14941 tggaataatg agcgaaaata tcttcgccgc cgccttcagg gcagatgaaa ccaaaccctt
    15001 tggcattgtt gaaccactta acagtaccct tttccatgct tcgacatcct tcgcaaatct
    15061 tatacaagta agatggaata aaccggggtc agagaggggg ctgttcaaaa cctcgccaac
    15121 tctagaaata caatttagag aattagggcg agccgtcaag catttgacag gggacaaggg
    15181 gcaggtatga atcaaaaatt tgaagcagtt aacgctattg acaggaatgt gacagatgtc
    15241 gctgatgcca acgatagatg atagttattt atcatctagt gttcgcgggt gttcgtggta
    15301 gtgttttgtg tgcattcatc gctatgatta taaaggaaaa ttttgtttta cttgttcgct
    15361 cttgttcgtc acggtttgcc cttgtacgat gcacggtgta gtcaatggtg tagttaattt
    15421 tgatgaccgg aaacgcgaaa catgagcagc gcaattcata gacttagcga cactctttta
    15481 cgaaaattaa gcggatcacc aaccacaaaa aacgcctttt ttaatgacgg tggcaatctt
    15541 agcgtaagac attccaccag cggcctgtta acctggtatt tcacttacag ggccggaact
    15601 ggcaggcagg tatcaccgga acgcctgaga ctgggcaact atcccgatct gagcctgaaa
    15661 gcagccaggg aaaaagcggc acagtgtcgc gcctggttgg cggaggggaa aaatccacgc
    15721 catgagctta atcacacagt gcaggaagcg ttagcccccg tgacggttaa ggacgcgctc
    15781 acttactggc ttgagtcgta tgcgaaggaa aagcgcactg accacgaatc actgaaaagc
    15841 cgtatcaata agcacataat cagccagatt ggtgctatgc cgctggataa atgcgagcta
    15901 cgccactggc tggcctgttt tgaccagctt gctaagcgta atccggtatc agccggcttt
    15961 ttgttgcagg tatgcaaaca ggcgcttaag tactgccgta aacgtcgcta cgccgtcagt
    16021 aacgttcttg atgatatggt cgtaggtgat gtgggaaaaa aagcagaaat aagcgagcga
    16081 gtgctaacaa acaaggagct tggcgagcta ctccgcgcac tggatgaaaa aattttcccg
    16141 ccgtactaca gcgctttaat tcgccttctg attgtcttcg gatgccgtac cactgagcta
    16201 agacgctccg aggttcggga atgggatttt aaagaaatgc tctggactgt gccgaaagaa
    16261 cacagcaaaa cgaaggtagc aatattcagg ccaataccgg aagcgatctt gccgttcgtc
    16321 atgaagctgg tggagcagaa caggcacacg ggcttattgc ttggagaact gaaagggcaa
    16381 tcatccgtag cagagtacgg aagaacggca cacagacgca ttaatcaagc cccctggtcg
    16441 ttgcacgaca tccggcacac atttacaacc atgctgaacg atttaggcgt ggatccgcat
    16501 attgttgaac agcttactgc ccaccagatg ccaggaatgc aacgcgtcta taaccactcc
    16561 cgttatctcg atgcaaagcg tgacgctctt aatctatggg tagaccgtct cgagcttctc
    16621 cagaacaatg atgaaaaaat cgttgttatg accccgcgaa tttaccccca aaattcttga
    16681 caaattacgg ctgttttttc tttaagagat tacagccgtg aatagatctt agaatatcgg
    16741 ggaatatctg caaatatctt ctctttatat gtgtcaacta tcgaaaaaaa ttgaaatgtc
    16801 ttcttctgag ttcttattct ctgtttcttg attgttccaa catctgatgg tttatctatt
    16861 tgtttttaat gaggtaattc aggtgacatg ccaaatgacg acattttttg ttgcgtcgct
    16921 taaaaactcg tgacacgatt ttaaacacta acgagcttct acgaacctta gcgaacaaag
    16981 cctttagcat ggttgtgttt ttaatttaag aggtgaaaaa gaatctaagt aatatccgat
    17041 ctcatatatc tacagctaag agaaaagttt atatacgtaa tttttatgta ggtttttgta
    17101 tgcgtgaaat aaatgaagat cgcgtaatcc gtgaggatga atgtcgtaag ttaactggag
    17161 tgtgtcgcac tacacgctac gaactggaga aaaaagggag ctttccgtct cgccgtaatc
    17221 ttggtgggcg ttctgtaggt tggctgctgt ccgaggttat ggaatgggtt aaaagccggg
    17281 atcgcattaa ttcaggcaag gcagcgtaaa ggggagtata tgacacataa aacaaaggcg
    17341 accgggggcg gtcgccaatg ggaaaacact aaacataagc ccgaacatca tagcgatttg
    17401 ctggctggtg ggcaatgtgt ccagtgtggt ggcttgctgg tggattgtca gccaggtggg
    17461 cggtttttgt ttgcttttaa gcgccgaggg tatttctcga caaaaggatc aagggcgaac
    17521 ttatcaggaa ctttaagatt ttcagtacgg gttaatgcct tatttatttc cctgcgggtt
    17581 ttctcacgct ggcgcatgat aattgataat gctttatttt gtagttcctg gttattcata
    17641 attatcacct ttaaaacaat gcgccgcagt acctcacacc acggcgctga tagtgattat
    17701 tctgattctt tggccttgcg gcgctggcgg tattcaactt ctcgctttaa tgctgctgtt
    17761 acaaactgcc ctgtactttc accaggcatt tttaccgcct caacattgtt cataacttca
    17821 tgcggaaccc ttgccgcaac tgtttgtgat tttgcgttta ctgcttttgt cgccatgttg
    17881 tgtacccctt acaaaaaaca aatgcagtat gcaggaaaaa aaataagtgt tcaacacttg
    17941 gcgtgtttaa cacctaggct taaattggtg ttcaacacct tgttgatgaa aggtgcagaa
    18001 acgacgaaac cccgcactgt tggagcagta acggggcttc taaccaccaa cgatagaaag
    18061 cgtatcgagg tagctatgtt aaatcatacc acacacccgc aagggcggga cccgcacaac
    18121 ctgaataaat acatctggcg ttttatcgcc ctgagcacag cacaaccacg cgttattacc
    18181 atcggggcca ccagcgaaca ggaagcccgc cagcaatccc cgactggctg ggtgatggta
    18241 ttggcctcca gatttcatca tcgctgtttg ttttttgtct gtctctcctc ggggtatggg
    18301 gttgtgccgt tccagcgtat gcggggccgt tcactgatga agcgctcaag cgggaaatta
    18361 tcggatggcg cggggggccg tacccgtcgt tcccgcctcc gctggcgtac cacgctggct
    18421 tcggtaattt cccctgattc gtttattttc ataaagagtt acctccgtgc tcatctgctc
    18481 tggcgttttc tgtttctgat gaagacttac agcggtagcg gcgtatctta ttttaagatt
    18541 tggtttaatt tctcgtgtct ggctttaatg acggcgcgta ataatatctg tcgctgcttt
    18601 gtgtttacgt ccagaactgt gggggataat atggatttgt tcatggtgct cacctgtaat
    18661 gcattgcgtc gtgatgcctc atatcacggc ggcggttatc gttttgttcg gattcgtccc
    18721 ctgatgaaat cctcaagcgg aaaatgttca gggcgaggta attcaggagg gacagggtta
    18781 ttactcatgc gctcacgttc ccttgcggcg cgtgcagcat tcagcttttg caggagcaca
    18841 tcaaacggca cccccttatc attcatggtc ccccctgtat atccgttgtt aactgatggt
    18901 tacaggtgca tttttcgcac aaactattta atcaacacat ccgaccacat tatcagagag
    18961 ccggaatata caggggcaat aatcccgcat ttgctggcct acgcacagct taccgctgat
    19021 atggcactga atgaggccaa agccatcctg attgctgatt gtgaatatgg ggttatgcgt
    19081 gatgatcgtt ttaatgccct gaaacaggaa tttgatggca caccggaaga tacagatatt
    19141 gcgctgttat gtgttgctga catggtaaaa gctgcatgtt ttttactgga aaccgccgaa
    19201 cattcaggaa cgggtagcga tattctcaat attgcgtcgg actatgctga atatgtggca
    19261 gaggcgcgtt acagaagaaa attccaggag gtggttagcc atgaataatc atgaagcgca
    19321 cttacctgta gtgcttaatg tgccatctga ttatacgggg cgcgttttga tattcctgga
    19381 taaagggaaa gtgaaatcgc aatgccgcct tagaaataat gaggttattg gttctctggc
    19441 tttttttctg aagcctgtat tcgcgccgga ataaagccgg aatcactgtc aggaaaataa
    19501 aaccatgaaa aagaaaaatt ctggctttac tgccagcggc ctcactcggc ctgaaatccg
    19561 tcccggcgat attttccgtg ataactacgg cggtacggta acgattaaag gcgtggcaga
    19621 acggcgcatc acctaccgcc atgaaggtta cgaatatgat tgcgtgatgc ctgtttatca
    19681 gtttgtgcgt gatttttctc ttgtgcaggc cgtaccgcgt aacgtgccca ccagcaggga
    19741 gaaagcacgc gccaatattc agaagatgaa aaccatgatt aacggattca ggggtaaaaa
    19801 atgaaactgg caccgaacgt aaaactgcag tcacgcggca taaaacacaa aggaactgaa
    19861 gtcattattt ttgcgggtag tgatgcctgg gcacacgcga aacaatggca ggaacatgac
    19921 gcgcgtatgg ccggagataa tgagcctcct gtgtggcttg gggagcagca gttatccgaa
    19981 ctggataagc tgcaaattgt gccggaaggc agaaaatccg tgcgtatatt cagggccgga
    20041 tatcttgcgc cagtaatgat aaaggcgatt ggtcagaagc tggcggcggc tggcgtacag
    20101 gacgcaaatt tttaccccga aggaatgcac ggtcagaagt gtgaaaactg gcggcgttat
    20161 ctgaatgctg agcgtgaaaa tattgctgca gggcttacca tgccggagca gaaaaatacg
    20221 caactggcac aaatggcaga cagtgagcgc gcacagatgc ttgctggtcg atttgatggc
    20281 gtttgtgtgc atccggaaag tgaaatcgtt cacgtatggc gcggcggggt atggtgtccg
    20341 gtcagcacaa tggaactgag ccgcgaaatg gtggcgatct attcagagca cagggccact
    20401 ttcagcaagc gcgtaatcaa taacgccgtg gaagcgttaa aagttattgc cgaaccaatg
    20461 ggcgagccgt ccggcgattt gctgccgttc gccaatggtg cgcttgacct gaaaacgggg
    20521 gaattttccc cgcacacgcc ggagaactgg atcaccacgc acaatggcat tgagtacacg
    20581 ccaccagcac ctggggagaa catccgcgat aacgcgccaa actttcataa atggcttgag
    20641 cacgcagccg gaaaagaccc gcgcaagatg atgcgtatat gtgccgcgct gtacatgatt
    20701 atggcgaacc ggtacgactg gcagatgttt attgaggcca ccggagacgg cgggagcggt
    20761 aaaagtacat tcacgcacat agccagcctt ctggcaggga aacagaacac ggtaagcgct
    20821 gagatgacat cgcttgatga tgctggtggg cgtgcgcagg ttgtcgggag tcgtcttatc
    20881 gtcctggcag accagccgaa atatacaggc gaaggaacgg gcatcaagaa aatcacgggc
    20941 ggtgaccccg tggaaattaa cccgaaatac gaaaagcgtt tcacggctgt aatcagggcg
    21001 gtggtgctgg cgaccaacaa taacccgatg atattcaccg aacgggccgg aggtgtggca
    21061 cgtcgtcgcg tgatttttcg tttcgacaat atcgtcagtg aggctgaaaa agacagggag
    21121 ctaccggaaa ggattgcggc tgaaatcccc gtcattatcc gccgattact ggcgaacttt
    21181 gcagaccctg aaaaggcgcg ggctttacta ctggaacagc gtgacggtga tgaagcactg
    21241 gcgataaagc agcaaacgga tccggttatt gagttttgcc agttcctgaa ttttctggag
    21301 gaagcacgtg gcctgatgat gggtggcggt ggcgattccg tgaagtacac gaccagaaac
    21361 agcctttacc gcgtctatct ggcgtttatg gcgtatgcag gcaggagcaa accgctgaac
    21421 gtggctgagt tcagcaaggc catgaagcca gcggcgaaag tttacggaca tgaatatata
    21481 acgcgcagga ttaaaggggt tacgcaaacc aacgtgacaa caacagacga ctgcgacgcg
    21541 tttttatagt tttagacaaa agccctctac cccttctacc ttaagaaaat aattacatat
    21601 atttcatgtt gatagattgg gtagatggca ggtagaaggt acttaaaagc cctctacacc
    21661 ctctaccata attcagggca tttgtgaggg ggtggtagat ggcaggtaga gggccaaaaa
    21721 agccctctac cccgctgaaa gccgcgccat tactgacctg aaagatgatt gtgtagagag
    21781 gtagaggagg tgcaccacaa cacaaaactt tttaaacgag ggggtaaaaa taaaaatgca
    21841 cacatcagga aaacttaata atcagaagaa accacataac cgcgccattg accttacaga
    21901 gcactggctg agggtggcga taaaaattat cgaccgcagc gcgggagaag gatacgcgaa
    21961 agcacatccc gaactgataa gcgcattcat gacaacggta gcggcaaatt ttgccacgct
    22021 gacagaacgg gagattgccg gagcggaaca ggtgacaacc atcaacgtta aaaccggaga
    22081 gcatacagca tgacagcaca gatagcggct tacgggcggc tggtggctga cccgcagtta
    22141 aagaccacca gcaaggggac acaaatggcg atggcgagta tggcggtccc ccttccgtgc
    22201 agccaggcag atgacggaac ggcgacgatg tggttatctg tgctggcgtt tggcagacag
    22261 gccgaagcac tggcaaagca ccgcaagggt gaactcctga gcgtggcggg taacatgcag
    22321 gtgagccagt ggaccggaca gaacggggaa acgcggcagg gctggcaggt tatcgcagac
    22381 agcgtaatca gtgcgcggac agtgcgaccg ggcggaaaaa cacgcaaaac cacaggcgca
    22441 caggggaata agccaccagc gggaggcgat gacccctacg gagatgatat acccttctga
    22501 atcaggcggg gagcaacatg aacaacagca caccgaagaa ccggacaata agcgacgcga
    22561 cagcagaaga actcgagagc aggggactgt ggcgacgggc tgccagaagg tggcaggatg
    22621 tgatgattac tcttcaggaa gacaggcaca gagaacttgc ggcaatgaga gcaagggaat
    22681 gtatcaggaa ggcaaagcgc ccgatacctg aaaaccgtta tgatgttcac gcagtcagaa
    22741 gggccgcaga caggacaata caacagatgg ggctaccgtc gcttactgat gaaatatggc
    22801 gtgattaccc tgataacgta aatgatgacg gttattaaca ggcggggaaa cacgacacca
    22861 gcgggagcga cacggggtag cagatgccgc tggtggaaca gcacaaaggg ggagaacatg
    22921 acaaagctga ccattaaccg aaaaccaaaa ggcatatacg gcacaccgca gaaaaagacg
    22981 caggaggctg aacagccgga taaaaccaca ccagcgcaaa agcagagccg cgagcagaaa
    23041 acacaacaga agacggcagc aaaacgcaaa tcagaaaaga gccggaaaaa aaatatcaga
    23101 cgcgttgaac gccttgccgc gctctggcct gaacttttca gccaggaatc gcccagaccg
    23161 cttaaggttg gcatatttga tgacctgatg gaggatatta ctgcgagagg tatttcatta
    23221 ggtcagggag cgcttcgcgc aacgctggcg tattatgtca gttcagtgcg atattaccgt
    23281 tccctgatgg ctggtggcgt gcgttacgac ctgaaaggcc atccgtgcgg ggaggtgaca
    23341 caggagcacc agttgaacgc ggggaagcga ctggatgaga tgaagaaaaa acagcaggag
    23401 cgcagaaaca tgaaggcaga aaataagcat aacggtaagc caccagcagg gcataatatt
    23461 attctgaaaa atgccagttc ctcgggcgta cggaaagcat aaaaccctga ccacatgaag
    23521 agacgaagcc ggagtagtcc ggctttattt tttatgggtc ctttccggca tgtggacccg
    23581 ttacggggcg tcgaccttgc ggattttcgc tatttatgag ccttttcggg ttggtggtgg
    23641 tggttttgtt gttcgctcta tctctatgaa taaaaatgga aagataacac caacacacca
    23701 acctgaagca ttaattaagt gggggtattg atgaaatcgc acctgatgaa taaaaaaaac
    23761 atggcgaaaa gctgccgagt aagcgccact gcgttcgata agtggggagt gattcccgtc
    23821 gagcgtaagg gccgtgagac gttttatgat gtcgccagtg taatagataa tcgggttaac
    23881 aatgcaatca gccagattac aaacgacaaa ggcgagattg acgatgatga acttttacga
    23941 gtcaggatca gattactgac agcacaggcg gaggcgcagg aacttaaaaa cgagcgcgat
    24001 cgcggtgacg tgattgatac agagttttgc atgtacgcgc tttcaaaact ggcgagtcag
    24061 atttcatcaa tcatggacag cctcccgctt actatgcaaa ggagcttccc acagatgacc
    24121 cccgccatgc tggatggcct gaaaaaagaa gttgtcagag cctgtaacgc atgcacaaaa
    24181 cttgatgaaa acatcccgcg aatgctgtcc gattatctga tggaaactac cggaaatgtg
    24241 cctgataagt ttcagccgga taaagacaag taacgtagtg cacaatgacc gaagccagtt
    24301 tactgactgg ctttcagcgt tgcgctggtg ggcgttatgc gtcagtgatg aacaaaaaac
    24361 aatcgaaatc gacaccgaaa aataaaacat caagtcatat caatatattg cattggtggt
    24421 gataacgaat aaaaatgcaa aaactagcct ttttccgcga tgctcccgcc ccgtggcagg
    24481 gcaccccaca aggaggaccc gccatcacta tggaggccat gaccatgacc gagaccgaag
    24541 cgctcgggat aatccgcagt attaccggaa tcagccagca ggctggcgaa caggaggcca
    24601 cgcagccgga cagcgtgata gccgaaaatt acgcgtgtgt tgttgctgag gtgatgcgcc
    24661 gtgatggtat tgagcttaac ggcgtggata tgcgcaacat acgaaccaga gtccttgagt
    24721 tgctggcata ccgtcgccgt tctcaacaac ggagggagag cgcgaaaaac acttatcagt
    24781 ggaagaagcc ggaacggttg cggcggtaga ttcctgatat tcaggaaaac cacgaatccg
    24841 ggggttttac tggcgcggtg tacacggtgg aacgcatagc gttacctgac ttattgcatt
    24901 gtaaaaagtt agaaaggcac ggcctgtaag atgggatgtt gtgagtagtt taaagctacc
    24961 cgcaaattct ttcgttgtgc tggtacaggc ggttaaaccg tcggtaactg gataatcccc
    25021 actgggggaa aagctggcta catccctcac atctgaggac tgataatgcg acattgcgct
    25081 ggctggtggg tgagttgcag atttgcaact cgaccatgaa actacggaaa ctacccgtag
    25141 tttgggtagt aagagtaacg cccagatttt ggggcttact cgcgataccc aaataaaggg
    25201 tatcggtgga agaaatatcg tttctcatat gtaagtaccg agggcggaat tccgccttcg
    25261 gttacttatt gtgctcatgc acagggaggg gcgggtcaaa tctctgtacc ctgacgtctt
    25321 caggactgcc cgccccatcg attttttata cccgcgaaaa atgaaaaacg cttcacgctg
    25381 gtgggcctga tgccgatatg gggagccgca taccggagaa tatccaccag cacggcaaca
    25441 gcagaatagc cccgacacag aaaaaccacg aatatggggt ttttgttatg gcatggtcat
    25501 gatgaccgca cctgctctga atggtggaaa tcaccacgca gatcatccac cagcttcatg
    25561 acggatagcc ggaaaatgat aatgattaaa gcgtgttgat ctttgttcgc ggttgttcga
    25621 tattgttcgt gaagcggtgt agttaatggt gtagttaata tcactgtttt tgacgggctt
    25681 gttatttgca acataatgaa aaataaagac taattaacta aatgatataa atgatagtta
    25741 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
    25801 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
    25861 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
    25921 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
    25981 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
    26041 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
    26101 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
    26161 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
    26221 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
    26281 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
    26341 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
    26401 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
    26461 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
    26521 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
    26581 atgtggtgaa ctttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
    26641 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
    26701 cgacgaacct gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt ggtcgtgaga
    26761 aggagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
    26821 tgggggaatc tggtgtcggt aaaaccgcga ttgcggaagg tcttgcctgg cgaattgttc
    26881 agggcgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
    26941 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
    27001 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
    27061 cgggtgcagc gtctggtggt caggtcgatg cggctaacct aatcaaaccg ttgctctcca
    27121 gcggtaaaat tcgtgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
    27181 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcgatcgaag
    27241 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
    27301 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt aacgatcgtc
    27361 atctgccgga taaagccatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
    27421 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
    27481 ttgcacgcat tccagagaag agtgtttctc agagtgatcg tgataccctg aaaaacctcg
    27541 gcgatcgctt gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
    27601 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggttggt tcgttcctgt
    27661 ttgccggccc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagctttgg
    27721 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
    27781 ttattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg actgatgcgg
    27841 tcatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg cacccggacg
    27901 tgttcaatat tctgttgcag gtgatggata acggtacgct gaccgataac aacggacgca
    27961 aagcagactt ccgtaacgtg gtgctggtga tgaccaccaa cgccggggta cgggaaactg
    28021 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
    28081 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg tttgatcatc
    28141 tgtcaaccga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
    28201 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
    28261 aaggttacga ccgggcaatg ggcgctcgtc cgatggcgcg tgtcatccag gacaacctga
    28321 aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
    28381 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
    28441 aggcggaagc agcgcattaa tctgattgtt aggtaggttg gtcaagtccg taatctcgaa
    28501 agaggttacg gactttttgt ttatggggtg gaggaggttc agaccctttt tttaatgatg
    28561 atggtaagtt gttgataatt agtgctgcgg gaaggtaagg ataaaaaagg gtgctgcagg
    28621 agaatgggat ggttttgctt tattaacaac gggctaaacg tgtagtattt gagttcactg
    28681 ccgtacaggc agcttagaaa ttcacaggta acatactcca cccgcccacc atgttcactg
    28741 ccgtacagac agataaaatg cgaaaaaaaa gctcg
//
LOCUS       DBBGLQ010000053        28333 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.053, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000053 DBBGLQ010000000
VERSION     DBBGLQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.053"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..293)
                     /locus_tag="ACIYSB_003146"
     CDS             complement(<1..293)
                     /locus_tag="ACIYSB_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGC5430137.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAA"
     gene            complement(364..2478)
                     /gene="fusA"
                     /locus_tag="ACIYSB_003147"
     CDS             complement(364..2478)
                     /gene="fusA"
                     /locus_tag="ACIYSB_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGC5430138.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2575..3045)
                     /gene="rpsG"
                     /locus_tag="ACIYSB_003148"
     CDS             complement(2575..3045)
                     /gene="rpsG"
                     /locus_tag="ACIYSB_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGC5430139.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3142..3516)
                     /gene="rpsL"
                     /locus_tag="ACIYSB_003149"
     CDS             complement(3142..3516)
                     /gene="rpsL"
                     /locus_tag="ACIYSB_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGC5430140.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3642..3929)
                     /gene="tusB"
                     /locus_tag="ACIYSB_003150"
     CDS             complement(3642..3929)
                     /gene="tusB"
                     /locus_tag="ACIYSB_003150"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGC5430141.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIILIDYTDFVRLTVKHPSQMAW"
     gene            complement(3937..4296)
                     /gene="tusC"
                     /locus_tag="ACIYSB_003151"
     CDS             complement(3937..4296)
                     /gene="tusC"
                     /locus_tag="ACIYSB_003151"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGC5430142.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4296..4682)
                     /gene="tusD"
                     /locus_tag="ACIYSB_003152"
     CDS             complement(4296..4682)
                     /gene="tusD"
                     /locus_tag="ACIYSB_003152"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGC5430143.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIADGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRAWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4682..5404)
                     /gene="yheO"
                     /locus_tag="ACIYSB_003153"
     CDS             complement(4682..5404)
                     /gene="yheO"
                     /locus_tag="ACIYSB_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5430144.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5571..6383)
                     /gene="fkpA"
                     /locus_tag="ACIYSB_003154"
     CDS             complement(5571..6383)
                     /gene="fkpA"
                     /locus_tag="ACIYSB_003154"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGC5430145.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATAADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6604..6822
                     /gene="slyX"
                     /locus_tag="ACIYSB_003155"
     CDS             6604..6822
                     /gene="slyX"
                     /locus_tag="ACIYSB_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGC5430146.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6871..7461)
                     /gene="slyD"
                     /locus_tag="ACIYSB_003156"
     CDS             complement(6871..7461)
                     /gene="slyD"
                     /locus_tag="ACIYSB_003156"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGC5430147.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7556..7756)
                     /gene="yheV"
                     /locus_tag="ACIYSB_003157"
     CDS             complement(7556..7756)
                     /gene="yheV"
                     /locus_tag="ACIYSB_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGC5430148.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7766..9571)
                     /gene="kefB"
                     /locus_tag="ACIYSB_003158"
     CDS             complement(7766..9571)
                     /gene="kefB"
                     /locus_tag="ACIYSB_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGC5430149.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKVGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9571..10122)
                     /gene="kefG"
                     /locus_tag="ACIYSB_003159"
     CDS             complement(9571..10122)
                     /gene="kefG"
                     /locus_tag="ACIYSB_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGC5430150.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10253..12166
                     /gene="yheS"
                     /locus_tag="ACIYSB_003160"
     CDS             10253..12166
                     /gene="yheS"
                     /locus_tag="ACIYSB_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5430151.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12166..13188
                     /gene="yheT"
                     /locus_tag="ACIYSB_003161"
     CDS             12166..13188
                     /gene="yheT"
                     /locus_tag="ACIYSB_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGC5430152.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13182..13400
                     /gene="yheU"
                     /locus_tag="ACIYSB_003162"
     CDS             13182..13400
                     /gene="yheU"
                     /locus_tag="ACIYSB_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGC5430153.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13454..14323
                     /gene="prkB"
                     /locus_tag="ACIYSB_003163"
     CDS             13454..14323
                     /gene="prkB"
                     /locus_tag="ACIYSB_003163"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGC5430154.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14378..14782)
                     /gene="yhfA"
                     /locus_tag="ACIYSB_003164"
     CDS             complement(14378..14782)
                     /gene="yhfA"
                     /locus_tag="ACIYSB_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGC5430155.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15084..15716
                     /gene="crp"
                     /locus_tag="ACIYSB_003165"
     CDS             15084..15716
                     /gene="crp"
                     /locus_tag="ACIYSB_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGC5430156.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15767..17857
                     /gene="yhfK"
                     /locus_tag="ACIYSB_003166"
     CDS             15767..17857
                     /gene="yhfK"
                     /locus_tag="ACIYSB_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGC5430157.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17924..19144)
                     /gene="argD"
                     /locus_tag="ACIYSB_003167"
                     /gene_synonym="dapC"
     CDS             complement(17924..19144)
                     /gene="argD"
                     /locus_tag="ACIYSB_003167"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGC5430158.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19230..19793)
                     /gene="pabA"
                     /locus_tag="ACIYSB_003168"
     CDS             complement(19230..19793)
                     /gene="pabA"
                     /locus_tag="ACIYSB_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGC5430159.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19825..20427)
                     /gene="fic"
                     /locus_tag="ACIYSB_003169"
     CDS             complement(19825..20427)
                     /gene="fic"
                     /locus_tag="ACIYSB_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGC5430160.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20417..20584)
                     /gene="yhfG"
                     /locus_tag="ACIYSB_003170"
     CDS             complement(20417..20584)
                     /gene="yhfG"
                     /locus_tag="ACIYSB_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGC5430161.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20689..21261)
                     /gene="ppiA"
                     /locus_tag="ACIYSB_003171"
                     /gene_synonym="rotA"
     CDS             complement(20689..21261)
                     /gene="ppiA"
                     /locus_tag="ACIYSB_003171"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGC5430162.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21532..22713
                     /gene="tsgA"
                     /locus_tag="ACIYSB_003172"
     CDS             21532..22713
                     /gene="tsgA"
                     /locus_tag="ACIYSB_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGC5430163.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22975..25518
                     /gene="nirB"
                     /locus_tag="ACIYSB_003173"
     CDS             22975..25518
                     /gene="nirB"
                     /locus_tag="ACIYSB_003173"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGC5430164.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVLCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25515..25841
                     /gene="nirD"
                     /locus_tag="ACIYSB_003174"
     CDS             25515..25841
                     /gene="nirD"
                     /locus_tag="ACIYSB_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGC5430165.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25967..26773
                     /gene="nirC"
                     /locus_tag="ACIYSB_003175"
     CDS             25967..26773
                     /gene="nirC"
                     /locus_tag="ACIYSB_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGC5430166.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26792..28165
                     /gene="cysG"
                     /locus_tag="ACIYSB_003176"
     CDS             26792..28165
                     /gene="cysG"
                     /locus_tag="ACIYSB_003176"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGC5430167.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6846 a         7604 c         7165 g         6718 t
ORIGIN      
        1 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
       61 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      121 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      181 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      241 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      301 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      361 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      421 cttcaggaat tccatagtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      481 gtatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      541 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      601 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      661 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
      721 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
      781 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
      841 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
      901 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
      961 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1021 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1081 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1141 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1201 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1261 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1321 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1381 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1441 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1501 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1561 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1621 caggtaatca attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1681 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttgatttc
     1741 tgcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     1801 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     1861 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     1921 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     1981 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2041 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2101 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2161 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2221 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2281 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2341 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2401 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2461 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2521 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2581 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2641 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     2701 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     2761 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     2821 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     2881 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     2941 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3001 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3061 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3121 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3181 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3241 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3301 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3361 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3421 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3481 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3541 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3601 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3661 gggtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat gtcgttcgaa
     3721 atttgaccag tcaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     3781 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac gccatcttgc
     3841 aataatagca gttcgtctcc ttcactgagc agacgcagca gcgcagcaaa atccgtcagc
     3901 cagggtgagc gatgtaatgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     3961 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4021 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4081 gtcgtacaga cccaacaatt taaaagtggc aatgtaatca cgcgccagca ctgcatcggg
     4141 cttttgtcct ggcagcagct gaaaaacgcc atcagctata aagaagacag ccagatcgtc
     4201 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cggcctgcgg ctgtaccatg
     4261 aggtgcagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4321 gtcagcgagg cttccgccag cgccccaagt ccgcttaagg taaatccctg ctgaaggttt
     4381 gacgaagcca gccccagtct tccggcctcc gtttcatcaa caacgccacg gcgtaatgct
     4441 gccgctacgc agatattcag cgccacacca tgttgcgcat tcagttgttg ccaggcccgt
     4501 acgaggtcaa attcgtcact tgccggagag gtcaattggt tagcgttata gaccccttcc
     4561 cgatagaaaa agacgctgct taactcatgg ccatctgcta tcagcgcctg cgcaaactga
     4621 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4681 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     4741 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcatcttta atatcgaaga
     4801 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     4861 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     4921 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     4981 taatctgcga gaagggaaca tcaagattca tattgatgca cagcagacca attacacgct
     5041 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5101 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5161 ccaggtcagt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5221 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5281 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5341 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5401 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5461 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5521 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5581 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5641 acatccagca gctctacgtc aaacaccagg gtagaattcg gagggatccc cggaacaccc
     5701 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     5761 ttcttcagac cttctgtcca acccgggata acaccgtcca gacggaaaga aagcggttca
     5821 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcact
     5881 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttctactac ctgataaacc
     5941 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6001 ccttttgctt cgttatcagc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6061 cgagcttcga atgcttgtag agtctgttcg atctcttggt cggagagttt gctcttatca
     6121 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6181 tcttgttctt ttagagagtt ttccatgtaa cgacccagcg aggcacccag tgcataagct
     6241 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagctgtagc aggttttgca
     6301 gcttcagcag caaaagtgat tggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6361 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6421 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6481 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6541 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6601 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6661 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     6721 aaactgcgcg atcatctgcg tctgctgacc gagaagttaa aagccagcca gccgtcgaac
     6781 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     6841 attccccgct tttttgtcac tttttcggta ttagtggcaa ccgcaaccgc cgttgccttt
     6901 accgccacag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccgccgca
     6961 gcaaccgtcg tggtcgtgat cgtggtggtg atcgtgcgcg ccgtgaacgt gaccatgagc
     7021 cagttcttct tcagtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7081 accggccagc atgtggttac catcaaccac gacgtgatcg tcttcaaccg cagtgatttc
     7141 aaccggtacc ggaccctggt cggtttcagc caggaaacgc atacctacct gcagttcatc
     7201 aacgcccata aatacgtctt taggaacacg ttgcaccagg ttttcgtcgt actgaccgta
     7261 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcatgac cttccagcgc
     7321 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7381 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7441 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7501 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7561 ccggatgaaa aatcccgatc acttgctcat ctttgcgaac gtgatcgcgg gcttctttgt
     7621 ctgcttctcg catctgatgt ccgcacttaa cacattcaac aatatcaata ttattttcgc
     7681 gccacatcgc cattgaatcc tgcgcctgac aggccgggca ttttgcgccc gcaataaaac
     7741 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     7801 gttcttgttg catttcacgc tggaaaatct cttccagttc gcgtcgggct tccctggcgc
     7861 gagaaatttg tacggtatcg gcatgcattg ggatgagctc tcgcagcatc cgcatatcca
     7921 ggcggcgaaa atgcagttgc gctcgctgtg cctgatgcgg atgcatgcca agcgtgacca
     7981 gcgtcttgcg ccccagctct aacgcactgg agaatgtttc acgggaaaac tgcgtcaccc
     8041 ctgcctgtaa taactcatgc gcttccacac gtccgcgcgc tcgcgcaaga atatgcaaat
     8101 gcggaaagtg ctgttggcat atttccacca gcttcatggt gtcttccggc tcgttacagg
     8161 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatcg acctgcgtgg
     8221 cgtcgccgta ataaactttg tagccgtatt tacgcatcag gttaacggcg ctgatatccc
     8281 gctccagcac ggtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8341 aacgcccgaa gcccacgaca atgacctggg gtttatcatc gttgacccac ggtttttcgt
     8401 cttcttcttc cggtccgtta aactggcggg atagccattt atccaccagc ttcatcagca
     8461 acggcgtggt catcatggaa agcgtcaccg tcaccagcaa caacgccatc tggtcgccct
     8521 ggaataagcg ttgtgaagaa gcggtagaaa agaggacaaa ggcaaactca ccaccctgac
     8581 tcaacacgcc agcaaactgc atccgctctg agctacgcac gccatacaat cgcgccagca
     8641 gatacagcac gagaattttc accgccacca gcacaaccac gcttatcact acccacaaca
     8701 gatgggtata aagcaccccg aggttgagtg acatgccgac agagataaag aacaaaccga
     8761 gcagcaagcc tttgaaggga tcgatagccg tttccagttc atggcgatat tcactttccg
     8821 ccagcagcac gcccgcaata aatgtaccga gcgccatcga cagccccagc gcatccataa
     8881 acaatgcgga acccaacacc agcagcagcg tcgcggcggt gaacacttcc cgcacgccag
     8941 aagctgcaat aaagcggaat accggacgca gtaaatagcg cccaccaatc agcatgccga
     9001 caaacgccag caccttcatg ccgaccttca tccagtcgaa atgttcgtct gccgaccccg
     9061 ccaacaacgg cactaacgcc agtgctggga ttactgccag atcctgaaac agcagaaccg
     9121 aaaacccgag ctggccggat tcgctgcgat tcatcccttt ctcacgcatc aattgcaacg
     9181 ccattgcagt tgaagacatc gcaaggccaa tgccaccgac caccgccgcc tgccaggcga
     9241 aatccgtcag catcaataat cccgccagca acgccgcgct taacagcacc tgtgccgcgc
     9301 ctacgccaaa aatcgaacgc cgcagttgcc aaagtttgga gggattcaac tcaaggccga
     9361 tgataaacat caggaatacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9421 taataaaccc cagcccccac gggccaattg caatccctgc cagcaaatat cccaacacag
     9481 cgccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacagca
     9541 ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9601 cagccagtca ccgtaggctc tggcgtggct cgccagctcc tgtgcgcttt gccgtctcgc
     9661 ccagtaaata atgatgggac ttaaccaatg catccggcac atgcccgccg ccagttcaaa
     9721 ggggcgcagc acatcgctca tcgggtagcg attcagcgcg tcataacggt aagcactttc
     9781 cggctcgccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttcctcccgg
     9841 cccgctggca aaaccacgac ttaatacccg gtccagccac tctttcagta gcgccgggca
     9901 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
     9961 ctcacggggg atatcaataa aaaaatcggg atagtgcgcg taaaggtcgt gcacggtaac
    10021 attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc
    10081 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10141 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10201 acattacatt atcataacga taagttaaca tagtctgaac atacggcacc ttatgattgt
    10261 tttctcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10321 catcaaccct gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatctaccct
    10381 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10441 ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg cgctggaata
    10501 tgtcattgac ggcgaccgtg aatatcgtca actagaagcg cagctacacg acgccaacga
    10561 acgtaacgac gggcacgcca ttgcgaccat tcatggcaag ctggatgcta ttgacgcatg
    10621 gagtattcgc tcccgtgctg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10681 ggagcgcccg gtaagtgatt tttccggggg ctggcgtatg cgtcttaacc ttgcccaggc
    10741 gctgatttgc cgttcagact tgctgctgct cgacgaaccg actaaccacc tcgatctcga
    10801 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    10861 tcacgaccgc gacttcctcg atccgatcgt cgataaaatt attcatatcg aacaacaaag
    10921 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    10981 gcagcaacaa gcgatgtacg aaagccagca ggaacgcgta gcgcatctgc aaagttatat
    11041 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgca ttaagatgct
    11101 cgagcgtatg gagctaattg cccccgcgca cgtcgacaac ccgttccgct ttagcttccg
    11161 cgcgccggaa agcctgccaa atccgttact gaagatggaa aaagtcagcg cgggctatgg
    11221 cgatcgcatt attctcgact cgattaaact gaacctggtg cccggctcgc gtattggtct
    11281 gttaggccgc aatggcgcgg gtaaatcgac attaatcaaa ctgttagccg gtgaacttgc
    11341 gccagtcagc ggtgaaattg gtctggcgaa agggatcaaa ctcggctact tcgcccagca
    11401 tcaacttgaa tacctgcgcg ccgacgaatc acctattcaa catctggcac gtttagcgcc
    11461 gcaggagctg gaacaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11521 agtaaccgaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11581 aattgtctgg cagcggccga atctgctgct gctcgacgaa ccgactaacc accttgacct
    11641 cgacatgcgt caggcactca ccgaagcatt aatcgagttt gaaggcgcgc tggttgtcgt
    11701 ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    11761 taaagtcgaa ccgttcgacg gcgatctaga agattatcaa cagtggttga gcgacgtaca
    11821 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaat gcgaacagcg cccaggcacg
    11881 taaagatcag aagcgtcggg aagctgagct gcgtgcgcaa acccagccac tgcgtaaaga
    11941 gattgcccgt ctggaaaaag agatggagaa gctgaacgcg caactggcgc aggcggaaga
    12001 gaaactcggc gacagcgaac tgtatgacca gagccgtaaa gcggagttga ccgcctgcct
    12061 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcat ggctggaagc
    12121 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataacg
    12181 acgaccgatg ccaatgaatt cagcagcagt gctgaattca tccccatgcg cggctttagc
    12241 aattgtcatc tgcaaaccat gctgccgcgt ctgtttcgtc gtcaggtgaa attcaccccg
    12301 tactggcagc ggctggagtt gcccgacggc gattttgtcg atcttgcgtg gagtgaaaac
    12361 cctgcacagg cgcaacataa acctcgtctg gtggtgtttc acgggctgga aggcagtctc
    12421 aacagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12481 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12541 ggcgaaaccg aagacgccag ttggttttta cgctggctgc aacgcgaatt tggtcatgcg
    12601 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tggcctgttt gctggcaaag
    12661 gaaggcaatg atctcccggt tgatgcggcg gtgattgtct ctgcgccgtt tatgctggaa
    12721 gcctgtagct atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    12781 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacgct gccgattaat
    12841 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctcat caccgccaga
    12901 attcacggct acgccgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctg
    12961 aaccggatcg ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat
    13021 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact gactgaacat
    13081 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13141 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13201 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13261 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13321 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat attatgccgc
    13381 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13441 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13501 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13561 agctgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat
    13621 ccgcaaagcg cgcgacgccg ggcggcatat cagctacttc ggccccgagg ctaacgactt
    13681 cggcctgctg gaacaaacct tcattgaata cggtcagagc ggcaaaggga aatctcgcaa
    13741 atatctgcat acctacgacg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    13801 ctggcaacct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    13861 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    13921 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    13981 agaagcagtg atggactcag tagtgcgttc aatggaagac tatatcaact acatcacacc
    14041 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc
    14101 gttcgcggca aaaggtatcc cgtcgctcga tgaaagcttt gtggtgatcc attttcgtaa
    14161 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14221 catcaatacg ttagtggtac cgggcggcaa aatgggtctg gcaatggaat taattatgct
    14281 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactctgct attacgccgg
    14341 ataaaatact atccggcttc acaacgggat agttaagtca cgcggcaacc acttcatacg
    14401 agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag
    14461 agagatcaac cgcacgcgca accgctgcgt ctttcaggtc gcgaccggtg acgataaaat
    14521 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14581 cttcacaatc gaccacatcc tgacgccctt tttgcaggat cgaaaccaca tcgatggcac
    14641 tgcaaccacc cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt
    14701 tgccgtccat taaaatctga tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    14761 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    14821 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt caggctgaag cgagacacca
    14881 ggagacacaa agcgaaagct atgctaaaac agtcaggatg ctacagtaat acattgatgt
    14941 actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag
    15001 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15061 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15121 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15181 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15241 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15301 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15361 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15421 ttctgatgcg tttgtctgca cagatggcgc gtcgtctgca agtcacttca gagaaagtgg
    15481 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aatctggcaa
    15541 aacaaccaga cgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15601 ttggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15661 atcagaacct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    15721 gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    15781 gatttatcac cccgatatca actatgcact tcgacaaacg ctggtgctat gtttgcccgt
    15841 ggccgttggg ttaatgcttg gcgaattacg attcggtctg ctcttctccc tcgttcctgc
    15901 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    15961 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag ctactactgg caaaagatgt
    16021 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgagctggg
    16081 gccattgcac gcaaaattgc ttcctgcatc gctgctcgcc gccattttta ccctcagttt
    16141 ggcgggatac atgccggtct gggaaccgtt gctcatctat gcgttgggca ctctctggta
    16201 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact
    16261 aagtctgctg taccgtgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca
    16321 gcacaccgac cctgaaaaag cgctgccgcc gctgctggtg cgccagcaaa aagcggtcga
    16381 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata atactgacta
    16441 caagcggatg ctgcgtattt tccaggaggc gctggattta caggaacata tttcggtcag
    16501 tttgcatcag ccggaagagg tgcaaaagct ggtcgagcgt agccatgcgg aagaagttat
    16561 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggctgatg acattcttta
    16621 ccatcgcctg ccaacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16681 ccgccagcat ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc
    16741 ccgcgtgctg cgcacccaaa aaccgctcta tgcccgtgac ttactggccg ataaacagcg
    16801 gcgaatgcca ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cggcgctacg
    16861 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcgctgca
    16921 tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacacaaa atggctatgg
    16981 cgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    17041 gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac
    17101 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17161 tattaccgca gtgtataccc tgcaactatt gtggttgaac ggcgagcaat acatccttcc
    17221 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17281 gccgcagtgg cagagcgggt tattgcgtaa aaacgcccat gatgctttag aagcctatca
    17341 ggaagcgatt cgcttgattc ttagcgagga tccgcaacct acgccactgg cctggcagcg
    17401 aatgcgggta aatcaggcac ataacactct gtataactca ttgaatcagg cgatgcagga
    17461 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17521 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17581 tgaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17641 actggagtat gacgaaccgg gtagttctgg cgatgccaat atcatggatg cgccggagat
    17701 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacagcggg ttattggtca
    17761 tctgaacacc atgcacacca tttcgtcgat ggcatggcgt cagcgaccgc atcacgggat
    17821 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatgc aggctaaacc atcggataag
    17881 gcattcacgc cgcatccgac atcttttgcc tgatgcttcg tatttacgcc ccaaccacct
    17941 tcgccaccgc gtgggcgaaa cgttgcatcc cttcatcgat atccgcatct tccaccacca
    18001 gcgacggtgc aaaacgcatc acatccggtc cggcattcag caccattacg ccagcctctg
    18061 cgcccgcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc tctgcgccaa
    18121 tcaacagccc cataccgcga atatcgctaa atacatcgta ctgctgatcg atcttctgca
    18181 gatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga
    18241 tgatatcaaa cgccgccccc gctactgcac aggccagagg attaccgccg taggtggaac
    18301 cgtgagaacc aggatgaaac gcagaagcaa tttccgccgt ggtcagcatg gcgctaatcg
    18361 ggaagccgcc gcctaacgct ttcgcagagg tcagaatatc cggcgtaacg ccgtagtgca
    18421 tgtaagcaaa caaatcgccg gtccgcccca tcccgcactg cacttcatca aacaccaata
    18481 atgcctgatg ttgatcgcac agctcgcgca agccctgcaa aaactctggc gtcgctgccg
    18541 tcacaccgcc ctcgccctgg atcggctcaa ccaccaccgc acaggtgtga tcatccatca
    18601 ccgctttcac tgcatggaga tcgttaaagg gaacgtggat gatgtctgcc ggtttcggcc
    18661 caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat
    18721 gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt
    18781 aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca
    18841 cgcgttcggc aaacgttgcc tcaatcagtt tacgcccaag acgcagcgcc ggttcattgg
    18901 tgaaaacgtt actgatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg
    18961 caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg acatactcct
    19021 tgccttgctg atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag
    19081 cataaatcgg caggatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg
    19141 ccatctcatg atcaccctgt tacgcataaa caaatgtgaa attataacca caaaatatgc
    19201 ataaaaaatc actaaatggc aatcagaaat cagcgatgca ggaaattagc cagcagttga
    19261 tgtccttgtt cgctaagaat actttctgga tggaactgca caccttccag atcccactgg
    19321 cgatggcgaa tccccataat ctcgcgggtt tcgctccagg ccgtcacgtc aaagcacgct
    19381 ggtaatgagt caggttccac caccagcgaa tggtagcgtg tcacggtaag tggatttgcc
    19441 agcccccgaa atacgccctc accgttatgt gtaatcggcg aggttttgcc gtgcatgacc
    19501 tttgcggcgc gcacaacttt accgccaaat gcctgcgcca ttgcctgatg accgaggcag
    19561 acgccaagaa tcggcaagcg cccggcatag tggcgaataa cgtcaaggga gatcccggct
    19621 tcatctggcg tacaggggcc aggtgagatg acaatttttt gtggtttaag ggcgtcgata
    19681 tccgccagcg tcaacgcatc gttgcgctta accagcacat ccgcccccag ttcacaaaag
    19741 tactggtaga ggttccaggt aaaagaatcg tagttatcta taagcaggat catggcggct
    19801 ccggtacaaa agaaccgcgc tattttactc agattccccg gcttcgctta ccactttgct
    19861 aaatatcatc tgcagtgcgg tgagatcccc cattgcccca ctctgatttg cctgattcca
    19921 ggcctctttt tcgataccct gccagctcag ttgatatccg gcatgaatcg ccagttgctc
    19981 gaagaagatc cgctgtgcca gaccacttcc cacccggaag ggatgcagca cgttgatttc
    20041 acaatagtaa tgcgccagcc gctcgacgaa cttcgctttc tccaggccaa ccagataacc
    20101 ttcttcctcc agatcctgca tcagggcatt gccctctttt tcgatataag caaagtggca
    20161 gaacggcgta tcaccctgat aaatatcaac ttcacgcagt tgccctgccc agtcgaaaat
    20221 atcctgatac agctggcgat ggatagttcg caaatgcggt aaaccgcgca ccagcggacc
    20281 aagctcaatg gtcgcagcac gcagcgccgt catttcgtaa gcggcctgtt ccagccgctg
    20341 ctgctggcgg atgttcagcc ggttacgcat gatatcaagg cctggataaa gatacggatc
    20401 gcgcccttcg ccgaatttat cgctcatagt gactcctcag ctcttcaagg cgcgctaaag
    20461 cctctgcggc agtaagagtg actaaaggca tctcgacgcc ttcaagacgg cgactggcct
    20521 gaaaattacg attacgctga agctcccaga gacgggactt ttgcttatcg gtgagtttct
    20581 tcacttgatg cctccctgaa cgtgtccatt tgccacaagt ataagcggca aatccgggtt
    20641 acgccgggag agcagaagcg cgggcaagat tgcccgcgcg agaaatcatt acggcaggac
    20701 tttagcggaa aggataacta ccggttttga cggcacattc tggtacggac caacgtcatg
    20761 agtcggcacc tgggaaatct tatcggcaac gtccatgcct ttcaccactt taccaaatac
    20821 cgcgtaaccg aaatcacgct gaccatggtc aaggaaggcg ttatcggcaa cgttgataaa
    20881 gaactggctg gtggcgctgt ctttgtcagc ggtacgtgcc atcgcgatgg tgccacgcgt
    20941 gttgcgcagg ccgttatcgg cttcattttt gattggcggg tttggttttt tctgctgcat
    21001 ctgctcggtg aaaccgccgc cctgaatcat aaagccagga atgacgcggt gaaaggtagt
    21061 gttgttataa aaaccgctgt tcacataatc gacaaagttt tgcacagaca ctggcgcttt
    21121 ttgtttatcc agctccagtt cgatgttacc agctgaggtt gtcaacaata cgtgcgggtc
    21181 ccctttcgct gccattgctg cgggagaaag agcagaaaga gcgaaaacag cagccatcgc
    21241 cgccagggtc gatttgaaca tgagatttcc ttaacaaaca gagcagaaaa aaagcaagtg
    21301 aagagattct aaatagcctt caagaacaag gccatccctt tacctaattt tacgtatctg
    21361 aaatatatgt aacctgacga tcacttttta ttccgtgatc aaaatcacct cttaaaatgc
    21421 aatttagcaa ccgattgcaa taaaacattt aaacagatca caaaatcacc taaaatcgcc
    21481 cgctcgcggc atatcccgct atgccatttt atttttgaac gcaggccaat catgactaac
    21541 agcaatcgca tcaagctcac atggattagc tttctctcct acgcactgac tggtgcgttg
    21601 gttattgtca ccgggatggt gatgggaaat atcgccgatt atttcaatct gcctgtttcc
    21661 agtatgagta ataccttcac cttcctcaac gccggcattt taatctctat cttcctcaac
    21721 gcctggctga tggaaatcgt cccgttgaaa acgcagttac gttttggctt tctcctgatg
    21781 gtgctggcgg ttgccggttt gatgttcagc cacagcctgg cgctgttctc ggcggcgatg
    21841 ttcattctcg gggtggtcag cggcatcacc atgtcgattg gtacattcct ggtaacacaa
    21901 atgtatgaag ggcgtcagcg cggttcccgc ctgttattta ccgactcctt cttcagtatg
    21961 gctgggatga ttttcccaat gatcgccgcg tttctactgg cgcgcagcat tgagtggtac
    22021 tgggtttatg cctgcatcgg gctggtgtat gtcgctattt ttattctgac cttcggctgt
    22081 gagttcccgg cgctgggcaa acatgcgcca aaaacggatg ctccggtaga gaaagaaaag
    22141 tgggggatcg gcgtactgtt tctctctgtt gcggcgttgt gctacatcct cggtcagtta
    22201 ggttttatct cctgggtgcc tgagtatgcc aaaggcctgg gcatgagcct gaacgacgcg
    22261 ggcacgctgg tgagtaactt ctggatgtca tacatggtcg gcatgtgggc gttcagcttt
    22321 attcttcgct tctttgattt gcaacgcatt ctgaccgtac tggctggtct ggctgcgatt
    22381 ctgatgtacg tctttaacac cggaacacca gcacatatgg cgtggtcaat tctcgctctg
    22441 ggcttcttct ccagcgcgat ctataccacc atcatcaccc tgggttcaca gcagaccaaa
    22501 gtaccgtcgc caaaactggt taactttgtc ctgacctgcg gaaccatcgg tactatgttg
    22561 acctttgtgg ttaccggccc gatcgttgaa catagcggtc cgcaggcggc actgcttaca
    22621 gcaaacggtc tgtacgctgt cgtctttgtg atgtgcttcc tgttaggttt cgtcagccgt
    22681 caccgtcagc ataacaccct gacctctcat taattgctca tgccggacgg cactatcgtc
    22741 gtccggcctt ttcctctctt cccccgctac gtgcatctat ttctataaac ccgctcattt
    22801 tgtctatttt ttgcacaaac atgaaatatc agacaattcc gtgacttaag aaaatttata
    22861 caaatcagca atatacccat taaggagtat ataaaggtga atttgattta catcaataag
    22921 cggggttgct gaatcgttaa ggtaggcggt aatagaaaag aaatcgaggc aaaaatgagc
    22981 aaagtcagac tcgcaattat cggtaacggt atggtcggcc atcgctttat cgaagatctt
    23041 cttgataaat ctgatgcggc caactttgat attaccgttt tctgtgaaga accgcgcatc
    23101 gcttatgacc gcgtacacct ctcgtcttac ttctctcacc acaccgccga agagctgtcg
    23161 ctggtgcgcg aaggcttcta cgagaaacac ggcatcaaag ttctggtcgg cgaacgcgct
    23221 atcaccatca accgtcagga gaaggtgatt cactccagcg ccggacgtac cgttttttat
    23281 gacaagctga tcatggcaac cggttcctac ccgtggatcc cgccaatcaa aggttctgat
    23341 actcaggact gctttgtcta tcgcactatt gaagacctca acgccattga atcctgcgcc
    23401 cgtcgcagca aacgcggtgc cgttgttggt ggcggcctgt taggtctgga agccgcaggc
    23461 gcgctgaaaa acttaggtat tgaaacccac gttatcgaat ttgcccctat gctgatggca
    23521 gaacagcttg atcagatggg cggcgagcag ctgcgtcgca aaatcgaaag tatgggcgtg
    23581 cgcgttcaca ccagcaaaaa cacccttgag attgtgcagg aaggtgttga agcgcgtaaa
    23641 accatgcgtt ttgccgacgg cagcgaactg gaagtcgact ttatcgtctt ctctaccggt
    23701 atccgtccgc gcgataagct ggcaacccag tgtggtctgg acgttgctcc gcgtgggggt
    23761 attgtcatta atgattcctg ccagacttcc gatccggata tctacgccat cggtgaatgc
    23821 gcaagctgga acaaccgtgt atttggtctg gtagcacctg gctacaaaat ggcgcaggtc
    23881 gccgttgacc atattctcgg tagcgaaaac gcctttgaag gtgctgacct tagcgccaag
    23941 ctgaaactgc tgggcgtaga cgtaggcggt attggtgatg cgcacggtcg cacgcctggc
    24001 gcacgtagct acgtttacct cgacgaaagt aaagagatct acaaacgcct gattgtcagc
    24061 gaagacaaca aaaccctgct cggtgcggta ctggtgggcg ataccagcga ctacggtaac
    24121 ctgctgcaac tggtgctgaa cgctatcgaa ctgccggaaa acccggattc cctgatcctg
    24181 ccagcacact cgggtagcgg caagccgtct atcggtgttg ataaactgcc ggacagcgcg
    24241 caaatctgct cctgcttcga cgtcaccaaa ggtgatctga ttgctgccat caacaaaggc
    24301 tgccacacag ttgcggcgct gaaagctgaa accaaagcgg gtactggctg cggtggctgt
    24361 atcccgctgg tcactcaggt actgaacgcg gaactggcga aacagggcat cgaagttaac
    24421 aacaacctgt gcgaacactt tgcttattcg cgtcaggaac tgttccattt gatccgcgtt
    24481 gaaggcatta aaaccttcga agaactgctg gcgaaacacg gcaaaggcta cggttgtgaa
    24541 gtttgtaaac caaccgtcgg ttcgctgctg gcctcctgct ggaacgaata cattctgaag
    24601 ccggaacata ctccgctgca ggattctaac gacaacttcc tcgctaacat ccagaaagac
    24661 ggcacctact cggtgatccc gcgttctccg ggcggtgaaa tcaccccgga agggctgatg
    24721 gcggtaggtc gtatcgcgcg tgaatttaat ctctacacca agatcactgg ctcccagcgt
    24781 ctggcgatgt ttggcgcaca gaaagacgat ctgccggaga tctggcgtca gctgattgaa
    24841 gccggcttcg aaaccggtca tgcctatgcg aaagcactgc gtatggcgaa aacctgcgtg
    24901 ggtagcacct ggtgccgcta cggcgttggc gacagcgtcg gcctcggcgt ggaactggaa
    24961 aaccgctaca aaggcatccg tacgccgcac aaaatgaagt tcggtgtctc cggctgtacc
    25021 cgtgaatgtt cagaagctca gggtaaagac gtgggtatta tcgccactga aaaaggctgg
    25081 aacctgtatg tttgcggtaa cggcggcatg aaaccgcgtc atgcggatct gctggcggcg
    25141 gatatcgatc gcgaaacgct gatcaaatat ctcgaccgct tcatgatgtt ctacatccgt
    25201 actgccgaca aactgacgcg taccgcaccg tggttagaaa acctcgaagg cggcatcgat
    25261 tacctgaaag cagtgatcat tgacgacaaa ctggggctga acgcacatct ggaagaagag
    25321 atggcgcgcc tgcgtgaagc ggtactgtgt gagtggactg aaacggtcaa tacgccgtct
    25381 gcgcagactc gcttcaaaca cttcatcaac agcgacaagc gtgacccgaa cgtgcagatg
    25441 gtgccagagc gcgaacagca ccgtccggca acgccgtatg aacgtatccc agtaactctg
    25501 gtggaggaca acgcatgagc cagtggaaag acatctgcaa aatcgatgac atcctgcctg
    25561 aaaccggcgt ctgcgcgctg ttaggtgacg agcaggtcgc gattttccgc ccgtatcaca
    25621 gcgatcaggt gtttgcgatc agcaacatcg acccgttctt cgagtccagc gtgctgtcac
    25681 gcggactgat tgcggaacac cagggcgagc tgtgggtcgc cagcccgctg aaaaaacagc
    25741 gttttcgctt aagcgacggc ttgtgcatgg aagacgaaca gttttccgtc aaacattacg
    25801 aagcgcgagt gaaagacggc gtggtgcagc tgcgcggtta atgttttaac gggaggcgca
    25861 atgcctcccc tttttgcatg gtcctgtaat aatcttcggt atattgcagg acatttttta
    25921 aactttttgt tttatttttt gtttttattt tttaaaggat aatcaaatgt ttacagacac
    25981 tattaataag tgtgcggcta acgctgcgcg cattgcacgc ctgtcggcaa ataacccgct
    26041 cggcttttgg gtcagctccg ccatggcggg cgcgtatgtg ggtcttggga tcatcctgat
    26101 tttcacgctc ggtaatttgc tcgatccatc cgtacgccct ttggtgatgg gcgcgacctt
    26161 tggtatcgcc ttaacgctgg tgattatcgc cggttctgaa ctgttcaccg gacacaccat
    26221 gttcctcacc tttggggtaa aagcgggcag catcagccac gggcaaatgt gggcaatcct
    26281 gccgcaaacc tggctgggta acctggtcgg ttccgtcttc gttgccatgc tctatagctg
    26341 gggcggcggt agcctgctgc cggtagatac cagcatcgtt cactccgtcg cgctggctaa
    26401 aaccactgca ccggcaatgg tactcttctt caaaggtgca ttgtgtaact ggctggtttg
    26461 cctggcaatc tggatggcgc tgcgcactga aggggcggcg aaatttatcg ctatctggtg
    26521 gtgtctgctg gcatttatcg cgtccggcta cgagcactct atcgctaaca tgacgctgtt
    26581 cgcgctctcc tggttcggca accacagcga agcctacacg ctggcgggta ttggtcataa
    26641 cctgctgtgg gtgacgctgg gtaatacttt atcaggtgcc gtattcatgg gattgggtta
    26701 ttggtatgct acgccgaaag cgaatcgtcc ggttgcggac aaatttaatc aaactgaaac
    26761 ggctgccggt taattactaa ggggttttta cgtggatcat ttgcctatat tttgccaatt
    26821 acgcgatcgc gactgtctga ttgtcggcgg tggtgatgtc gcggaacgca aagcaaggtt
    26881 gctgttagac gcaggcgctc gcttaacggt gaatgcatta gcgtttattc cacagttcac
    26941 cgcatgggca gatgcaggca tgttaaccct cgtcgaaggg ccatttgatg aaagccttct
    27001 cgacacctgc tggctggcga ttgcagcgac ggatgatgac gcgcttaacc agcgcgtcag
    27061 cgaagccgct gaagctcgtc gcatcttctg taacgtggtc gatgcgccga aagccgccag
    27121 ctttattatg ccgtcgatta ttgaccgctc accgctcatg gtagcggtct cctctggcgg
    27181 cacctctccg gttctggcac gcctgttgcg cgaaaaactt gaatcactgc tgccgttaca
    27241 tctgggccag gtagcgaaat acgccgggca attacgcggg cgagtgaaac aacagttcgc
    27301 cacgatgggt gagcgtcgcc gtttctggga gaaattgttc gttaacgacc gcctggcgca
    27361 gtcgctggca aacaacgatc agaaagccat tactgaaacg accgaacagt taatcaacga
    27421 accgctcgac catcgcggtg aagtggtgct ggttggtgca ggtccgggcg atgccgggct
    27481 gctgacactg aaaggactgc aacaaattca gcaggcagat gtggtggtct acgaccgtct
    27541 ggtttctgac gatattatga atctggtacg ccgcgatgcg gaccgtgttt tcgtcggcaa
    27601 acgcgcggga taccactgcg taccccagga agagattaac cagatcctgc tgcgggaagc
    27661 gcaaaaaggc aaacgcgtgg tgcggctgaa aggtggcgat ccgtttattt ttggccgtgg
    27721 tggcgaagag ctggaaacac tgtgcaacgc gggtattccg ttctcggtgg ttccgggtat
    27781 taccgcagct tctggttgct ctgcctattc gggtattcca ctcacgcatc gcgattatgc
    27841 ccagagcgta cgcttaatta ccggacactt aaaaaccggt ggcgagctgg actgggaaaa
    27901 cctggcggca gaaaaacaga cgctggtgtt ctatatgggg ttgaatcagg ccgcgactat
    27961 tcagcaaaag ctgattgaac acggaatgcc aggcgaaatg ccggtggcaa ttgtcgaaaa
    28021 cggtacggca gtcacgcagc gcgtgattga cggtacgctc acacagctgg gagaactggc
    28081 gcagcaaatg aacagtccat cgctaattat tattggtcgg gttgttggcc tgcgcgataa
    28141 actgaactgg ttctccaacc attaatttaa cccgggccag agaattctgg ccttcttaat
    28201 gactcttttt atatattcag caaataaaac atagcccctt aataaatatt atctgctaat
    28261 gggttatatt caccgttaca gcgacttaca aaattaagtc attcgctcac tgacgccaat
    28321 taaatattcc tgt
//
LOCUS       DBBGLQ010000054        28175 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.054, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000054 DBBGLQ010000000
VERSION     DBBGLQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.054"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(190..1473)
                     /gene="gltA"
                     /locus_tag="ACIYSB_003177"
     CDS             complement(190..1473)
                     /gene="gltA"
                     /locus_tag="ACIYSB_003177"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGC5430168.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1679..1786)
                     /locus_tag="ACIYSB_003178"
     CDS             complement(1679..1786)
                     /locus_tag="ACIYSB_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430169.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2167..2571
                     /gene="sdhC"
                     /locus_tag="ACIYSB_003179"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2167..2571
                     /gene="sdhC"
                     /locus_tag="ACIYSB_003179"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGC5430170.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2565..2912
                     /gene="sdhD"
                     /locus_tag="ACIYSB_003180"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2565..2912
                     /gene="sdhD"
                     /locus_tag="ACIYSB_003180"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGC5430171.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2912..4678
                     /gene="sdhA"
                     /locus_tag="ACIYSB_003181"
     CDS             2912..4678
                     /gene="sdhA"
                     /locus_tag="ACIYSB_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGC5430172.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4694..5410
                     /gene="sdhB"
                     /locus_tag="ACIYSB_003182"
     CDS             4694..5410
                     /gene="sdhB"
                     /locus_tag="ACIYSB_003182"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGC5430173.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEADEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5711..8512
                     /gene="sucA"
                     /locus_tag="ACIYSB_003183"
     CDS             5711..8512
                     /gene="sucA"
                     /locus_tag="ACIYSB_003183"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGC5430174.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8527..9744
                     /gene="odhB"
                     /locus_tag="ACIYSB_003184"
     CDS             8527..9744
                     /gene="odhB"
                     /locus_tag="ACIYSB_003184"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGC5430175.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            10110..11276
                     /gene="sucC"
                     /locus_tag="ACIYSB_003185"
     CDS             10110..11276
                     /gene="sucC"
                     /locus_tag="ACIYSB_003185"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGC5430176.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11276..12145
                     /gene="sucD"
                     /locus_tag="ACIYSB_003186"
     CDS             11276..12145
                     /gene="sucD"
                     /locus_tag="ACIYSB_003186"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGC5430177.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12249..12971)
                     /gene="mngR"
                     /locus_tag="ACIYSB_003187"
     CDS             complement(12249..12971)
                     /gene="mngR"
                     /locus_tag="ACIYSB_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HGC5430178.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITPQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            13140..15056
                     /gene="mngA"
                     /locus_tag="ACIYSB_003188"
     CDS             13140..15056
                     /gene="mngA"
                     /locus_tag="ACIYSB_003188"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HGC5430179.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            15074..17707
                     /gene="mngB"
                     /locus_tag="ACIYSB_003189"
     CDS             15074..17707
                     /gene="mngB"
                     /locus_tag="ACIYSB_003189"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGC5430180.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDVMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            18554..20122
                     /gene="cydA"
                     /locus_tag="ACIYSB_003190"
     CDS             18554..20122
                     /gene="cydA"
                     /locus_tag="ACIYSB_003190"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGC5430181.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            20138..21277
                     /gene="cydB"
                     /locus_tag="ACIYSB_003191"
     CDS             20138..21277
                     /gene="cydB"
                     /locus_tag="ACIYSB_003191"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGC5430182.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            21292..21405
                     /gene="cydX"
                     /locus_tag="ACIYSB_003192"
     CDS             21292..21405
                     /gene="cydX"
                     /locus_tag="ACIYSB_003192"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGC5430183.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            21405..21698
                     /gene="ybgE"
                     /locus_tag="ACIYSB_003193"
     CDS             21405..21698
                     /gene="ybgE"
                     /locus_tag="ACIYSB_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGC5430184.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            21848..22252
                     /gene="ybgC"
                     /locus_tag="ACIYSB_003194"
     CDS             21848..22252
                     /gene="ybgC"
                     /locus_tag="ACIYSB_003194"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGC5430185.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            22249..22941
                     /gene="tolQ"
                     /locus_tag="ACIYSB_003195"
     CDS             22249..22941
                     /gene="tolQ"
                     /locus_tag="ACIYSB_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGC5430186.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AASEAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            22945..23373
                     /gene="tolR"
                     /locus_tag="ACIYSB_003196"
     CDS             22945..23373
                     /gene="tolR"
                     /locus_tag="ACIYSB_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGC5430187.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            23438..24637
                     /gene="tolA"
                     /locus_tag="ACIYSB_003197"
     CDS             23438..24637
                     /gene="tolA"
                     /locus_tag="ACIYSB_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGC5430188.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKKKAEAEAAKAVAEA
                     QKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKKAAAEKAAADKKAAA
                     EKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIF
                     GELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDAS
                     SYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKN
                     APLDFKP"
     gene            24770..26062
                     /gene="tolB"
                     /locus_tag="ACIYSB_003198"
     CDS             24770..26062
                     /gene="tolB"
                     /locus_tag="ACIYSB_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGC5430189.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKSPAWSPYL"
     gene            26097..26618
                     /gene="pal"
                     /locus_tag="ACIYSB_003199"
     CDS             26097..26618
                     /gene="pal"
                     /locus_tag="ACIYSB_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGC5430190.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            26628..27419
                     /gene="cpoB"
                     /locus_tag="ACIYSB_003200"
     CDS             26628..27419
                     /gene="cpoB"
                     /locus_tag="ACIYSB_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGC5430191.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGNANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            27584..27659
                     /locus_tag="ACIYSB_003201"
     tRNA            27584..27659
                     /locus_tag="ACIYSB_003201"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27617..27619,aa:Lys,seq:ttt)
     gene            27795..27870
                     /locus_tag="ACIYSB_003202"
     tRNA            27795..27870
                     /locus_tag="ACIYSB_003202"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27828..27830,aa:Val,seq:tac)
     gene            27873..27948
                     /locus_tag="ACIYSB_003203"
     tRNA            27873..27948
                     /locus_tag="ACIYSB_003203"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27906..27908,aa:Lys,seq:ttt)
     gene            28098..28173
                     /locus_tag="ACIYSB_003204"
     tRNA            28098..28173
                     /locus_tag="ACIYSB_003204"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28131..28133,aa:Val,seq:tac)
BASE COUNT         6845 a         6908 c         7624 g         6798 t
ORIGIN      
        1 tgcgactacc atgaagtttt taattgaaag tattgggttg ctgataattt gagctgttct
       61 attcttttta aatatctata taggtctgtt aatggatttt atttttacaa ttttttgtgt
      121 ttaggcatat aaaaatcaac ccgccatatg aacggcgggt taaaatattt acaacttagc
      181 aatcaaccat taacgcttga tatcgctttt aaagtcgcgt ttttcatatc ctgtatacag
      241 ctgacgcgga cgggcaatct tcataccgtc actgtgcatt tcgctccagt gggcgatcca
      301 gccaacggta cgtgccattg cgaaaatgac ggtgaacatg gaagacggaa tacccatcgc
      361 tttcaggatg ataccagagt agaaatcgac gttcgggtac agtttcttct cgataaagta
      421 cgggtcgttc agcgcgatgt tttccagctc catagccact tccagcaggt catccttcgt
      481 gcccagctct ttcagcactt catggcaggt ttcacgcatt acggtggcgc gcgggtcgta
      541 atttttgtac acgcggtgac cgaagcccat caggcggaaa gaatcatttt tgtctttcgc
      601 acgacgaaca aattccggaa tgtgtttaac ggagctgatt tcttccagca ttttcagcgc
      661 cgcttcgtta gcaccgccgt gcgcaggtcc ccacagtgaa gcaatacctg ctgcgataca
      721 ggcaaacggg ttcgcacccg aagagccagc ggtacgcacg gtggaggtag aggcgttctg
      781 ttcatggtca gcgtgcagga tcagaatacg gtccatagca cgttccagaa tcggattaac
      841 ttcatacggt tcgcacggcg tggagaacat catattcagg aagttaccgg cgtaggagag
      901 atcgttgcgc gggtaaacaa atggctgacc aatggaatac ttgtaacaca tcgcggccat
      961 ggtcggcatt ttcgacagca ggcggaacgc ggcaatttca cggtgacgag gattgttaac
     1021 atccagcgag tcgtgataga acgccgccag cgcgccggta ataccacaca tgactgccat
     1081 tggatgcgag tcgcgacgga aagcatggaa cagacgggta atctgctcgt ggatcatggt
     1141 atgacgggtc accgtagttt taaattcgtc atactgttcc tgagtcggtt tttcaccatt
     1201 cagcaggatg taacaaactt ccaggtagtt agaatcggtc gccagctgat cgatcgggaa
     1261 accgcggtgc agcaaaatac cttcatcacc atcaataaaa gtaattttag attcgcagga
     1321 tgcggttgaa gtgaagcctg ggtcaaaggt gaacacacct tttgaaccga gagtacggat
     1381 atcaataaca tcttgaccca gcgtgccttt cagcacatcc agttcaacag ctgtatcccc
     1441 gttgagggtg agttttgctt ttgtatcagc catttaaggt ctccttagcg ccttattgcg
     1501 taagactgcc ggaacttaaa tttgccttcg cacatcaacc tggctttacc cgttttttat
     1561 ttggctcgcc gctctgtgaa agaggggaaa acctgggtac agagctctgg gcgcttgcag
     1621 gtaaaggatc cattgatgac gaataaatgg cgaatcaagt acttagcaat ccgaattatt
     1681 aaacttgtct accactaata actgtcccga atgaattggt caatacttcc acactgttac
     1741 ataagttaat cttaggtgaa ataccgactt cataactttt acgcattata tgcttttcct
     1801 ggtaatgttt gtaacaactt tgttgaatga ttgtcaaatt agatgattaa aaattaaata
     1861 aatgttgtta tcgtgacctg gatcactgtt caggataaaa cccgacaaac tatatgtagg
     1921 ttaattgtaa tgattttgtg aacagcctat actgccgcca ggtctccgga acaccctgca
     1981 atcccgagcc acccagcgtt gtaacgtgtc gttttcgcat ctggaagcag tgttttgcat
     2041 gacgcgcagt tatagaaagg acgctgtctg acccgcaagc agaccggagg aaggaaatcc
     2101 cgacgtctcc aggtaacaga aagttaacct ctgtgcccgt agtccccagg gaataataag
     2161 aacagcatgt gggcgttatt catgataaga aatgtgaaaa aacaaagacc tgttaatctg
     2221 gacctacaga ccatccggtt ccccatcacg gcgatagcgt ccattctcca tcgcgtttcc
     2281 ggtgtgatca cctttgttgc agtgggcatc ctgctgtggc ttctgggtac cagcctctct
     2341 tcccctgaag gtttcgagca agcttccgcg attatgggca gcttcttcgt caaatttatc
     2401 atgtggggca tccttaccgc tctggcgtat cacgtcgtcg taggtattcg ccacatgatg
     2461 atggattttg gctatctgga agaaacattc gaagcgggta aacgctccgc caaaatctcc
     2521 tttgttatta ctgtcgtgct ttcacttctc gcaggagtcc tcgtatggta agcaacgcct
     2581 ccgcattagg acgcaatggc gtacatgatt tcatcctcgt tcgcgctacc gctatcgtcc
     2641 tgacgctcta catcatttat atggtcggtt ttttcgctac cagtggcgag ctgacatatg
     2701 aagtctggat cggtttcttc gcctctgcgt tcaccaaagt gttcaccctg ctggcgctgt
     2761 tttctatctt gatccatgcc tggatcggca tgtggcaggt gttgaccgac tacgttaaac
     2821 cgctggcttt gcgcctgatg ctgcaactgg tgattgtcgt tgcactggtg gtttacgtga
     2881 tttatggatt cgttgtggtg tggggtgtgt gatgaaattg ccagtcagag aatttgatgc
     2941 agttgtgatt ggtgccggtg gcgcaggtat gcgcgcggcg ctgcaaattt cccagagcgg
     3001 ccagacctgt gcgctgctct ctaaagtctt cccgacccgt tcccataccg tttctgcgca
     3061 aggcggcatt accgttgcgc tgggtaatac ccatgaagat aactgggaat ggcatatgta
     3121 cgacaccgtg aaagggtcgg actatatcgg tgaccaggac gcgattgaat atatgtgtaa
     3181 aaccgggccg gaagcgattc tggaactcga acacatgggc ctgccgttct cgcgtctcga
     3241 tgatggtcgt atctatcaac gtccgtttgg cggtcagtcg aaaaacttcg gcggcgagca
     3301 ggcggcacgc actgcggcag cagctgaccg taccggtcac gcactgttgc acacgcttta
     3361 tcagcagaac ctgaaaaacc acaccaccat tttctccgag tggtatgcgc tggatctggt
     3421 gaaaaaccag gatggcgcgg tggtgggttg taccgcactg tgcatcgaaa ccggtgaagt
     3481 ggtttatttc aaagcccgcg ctaccgtgct ggcgactggc ggagcagggc gtatttatca
     3541 gtccaccacc aacgcccaca ttaacaccgg cgacggtgtc ggcatggcta tccgtgccgg
     3601 cgtaccggtg caggatatgg aaatgtggca gttccacccg accggcattg ccggtgcggg
     3661 cgtactggtc accgaaggtt gccgtggtga aggcggttat ctgctgaaca aacatggcga
     3721 acgttttatg gagcgttatg cgccgaacgc caaagacctg gcgggccgtg acgtggttgc
     3781 gcgttccatc atgatcgaaa tccgtgaagg tcgcggctgt gatggtccgt gggggccaca
     3841 cgcgaaactg aaactcgatc acctgggtaa agaagttctc gaatcccgtc tgccgggtat
     3901 cctggagctt tcccgtacct tcgctcacgt cgatccggtg aaagagccga ttccggttat
     3961 cccaacctgt cactacatga tgggcggtat tccgaccaaa gttaccggtc aggcgctgac
     4021 tgtgaatgag aaaggcgaag atgtggttgt tccgggactg tttgccgttg gtgaaatcgc
     4081 ttgtgtatcg gtacacggcg ctaaccgtct gggcggcaac tcgctgctgg acctggtggt
     4141 ctttggtcgc gcggcaggtc tgcatctgca agagtctatc gccgagcagg gcgcactgcg
     4201 cgatgccagc gagtctgatg ttgaagcgtc tctggatcgc ctgaaccgct ggaacaataa
     4261 tcgtaacggt gaagatccgg tggcgatccg taaagcgctg caagaatgta tgcagcataa
     4321 cttctcggtc ttccgtgaag gtgatgcgat ggcgaaaggg cttgagcagt tgaaagtgat
     4381 ccgcgagcgt ctgaaaaatg cccgtctgga tgacacttcc agcgagttca acacccagcg
     4441 cgttgagtgc ctggaactgg ataacctgat ggaaacggcg tatgcaacgg ctgtttctgc
     4501 caacttccgt accgaaagcc gtggcgcgca tagccgcttc gacttcccgg atcgtgatga
     4561 tgaaaactgg ctgtgccact ccctgtatct gccagagtcg gaatccatga cgcgccgaag
     4621 cgtcaacatg gaaccgaaac tgcgcccggc attcccgccg aagattcgta cttactaatg
     4681 cggagacagg aaaatgagac tcgagttttc aatttatcgc tataacccgg atgttgatga
     4741 tgctccgcgt atgcaggatt acaccctgga agcggatgaa ggtcgcgaca tgatgctgct
     4801 ggatgcgctt atccagctaa aagagaaaga tcccagcctg tcgttccgcc gctcctgccg
     4861 tgaaggtgtg tgcggttccg acggtctgaa catgaacggc aagaatggtc tggcctgtat
     4921 taccccgatt tcggcactca accagccggg caagaagatt gtgattcgcc cgctgccagg
     4981 tttaccggtg atccgcgatt tggtggtaga catgggacaa ttctatgcgc aatatgagaa
     5041 aattaagcct tacctgttga ataatggaca aaatccgcca gctcgcgagc atttacagat
     5101 gccagagcag cgcgaaaaac tcgacgggct gtatgaatgt attctctgcg catgttgttc
     5161 aacctcttgt ccgtctttct ggtggaatcc cgataagttt atcggcccgg caggcttgtt
     5221 agcggcatat cgtttcctga ttgatagccg tgataccgag actgacagcc gcctcgacgg
     5281 tttgagtgat gcattcagcg tattccgctg tcacagcatc atgaactgcg tcagtgtatg
     5341 tccgaagggg ctgaacccga cgcgcgccat cggccatatc aagtcgatgt tgttgcaacg
     5401 taatgcgtaa accgtaggcc tgataagacg cgcaagcgtc gcatcaggca accagtgccg
     5461 gatgcggcgt gaacgcctta tctggcctac aagtcattac ccgtaggcct gataagcgca
     5521 gcgcatcagg cgtaacaaag aaatgcagga aatctttaaa aactgcccct gacactaaga
     5581 cagtttttaa aggttccttc gcgagccact acgtagacaa gagctcgcaa gtgaaccccg
     5641 gcacgcacat cactgtgcgt ggtagtatcc acggcgaagt aagcataaaa aagatgctta
     5701 agggatcacg atgcagaaca gcgctttgaa agcctggttg gactcttctt acctctctgg
     5761 cgcaaaccag agctggatag aacagctcta tgaagacttc ttaaccgatc ctgactcggt
     5821 tgacgctaac tggcgttcga cgttccagca gttacctggt acgggagtca aaccggatca
     5881 attccactct caaacgcgtg aatatttccg ccgcctggcg aaagacgctt cacgttactc
     5941 ttcaacgatc tccgaccctg acaccaatgt gaagcaggtt aaagtcctgc agctcattaa
     6001 cgcataccgc ttccgtggtc accagcatgc gaatctcgat ccgctgggac tgtggcagca
     6061 agataaagtg gccgatctgg atccgtcttt ccacgatctg accgaagcag acttccagga
     6121 gaccttcaac gtcggttcat ttgccagcgg caaagaaacc atgaaactcg gcgagctgct
     6181 ggaagccctc aagcaaacct actgcggccc gattggtgcc gagtatatgc acattaccag
     6241 caccgaagaa aaacgctgga tccaacagcg tatcgagtct ggtcgcgcga ctttcaatag
     6301 cgaagagaaa aaacgcttct taagcgaact gaccgccgct gaaggtcttg aacgttacct
     6361 cggcgcaaaa ttccctggcg caaaacgctt ctcgctggaa ggcggtgacg cgttaatccc
     6421 gatgcttaaa gagatgatcc gccacgctgg caacagcggc acccgcgaag tggttctcgg
     6481 gatggcgcac cgtggtcgtc tgaacgtgct ggtgaacgtg ctgggtaaaa aaccgcaaga
     6541 cttgttcgac gagttcgccg gtaaacataa agaacacctc ggcacgggtg acgtgaaata
     6601 ccacatgggc ttctcgtctg acttccagac cgatggcggc ctggtgcacc tggcgctggc
     6661 gtttaacccg tctcaccttg agattgtaag cccggtagtt atcggttctg ttcgtgcccg
     6721 tctggacaga cttgatgagc cgagcagcaa caaagtgctg ccaatcacca tccacggtga
     6781 cgccgcagtg accgggcagg gcgtggttca ggaaaccctg aacatgtcga aagcgcgtgg
     6841 ttatgaagtt ggcggtacgg tacgtatcgt tatcaacaac caggttggtt tcaccacctc
     6901 taatccgctg gatgcccgtt ctacgccgta ctgtactgat atcggtaaga tggttcaggc
     6961 cccgattttc cacgttaacg cggacgatcc ggaagccgtt gcctttgtga cccgtctggc
     7021 gctcgatttc cgtaacacct ttaaacgtga tgtcttcatc gacctggtgt gctaccgccg
     7081 tcacggccac aacgaagccg acgagccgag cgcaacccag ccgctgatgt atcagaaaat
     7141 caaaaaacat ccgacaccgc gcaaaatcta cgctgacaag ctggagcagg aaaaagtggc
     7201 gacgctggaa gatgccaccg agatggttaa cctgtaccgc gatgcgctgg atgctggcga
     7261 ttgcgtagtg gcagagtggc gtccgatgaa catgcactct ttcacctggt cgccgtacct
     7321 caaccacgaa tgggacgaag agtacccgaa caaagttgag atgaagcgcc tgcaggagct
     7381 ggcgaaacgc atcagcacgg tgccggaagc agttgaaatg cagtctcgcg ttgccaagat
     7441 ttatggcgat cgccaggcga tggctgccgg tgagaaactg ttcgactggg gcggtgcgga
     7501 aaacctcgct tacgccacgc tggttgatga aggcattccg gttcgcctgt cgggtgaaga
     7561 ctccggtcgc ggtaccttct tccaccgcca cgcggtgatc cacaaccagt ctaacggttc
     7621 cacttacacg ccgctgcaac atatccataa cgggcagggc gcgttccgtg tctgggactc
     7681 cgtactgtct gaagaagcag tgctggcgtt tgaatatggt tatgccaccg cagaaccacg
     7741 cactctgacc atctgggaag cgcagttcgg tgacttcgcc aacggtgcgc aggtggttat
     7801 cgaccagttc atctcctctg gcgaacagaa atggggccgg atgtgtggtc tggtgatgtt
     7861 gctgccgcac ggttacgaag ggcaggggcc ggagcactcc tccgcgcgtc tggaacgtta
     7921 tctgcaactt tgtgctgagc aaaacatgca ggtttgcgta ccgtctaccc cggcacaggt
     7981 ttaccacatg ctgcgtcgtc aggcgctgcg cgggatgcgt cgtccgctgg tcgtgatgtc
     8041 gccgaaatcc ctgctgcgtc atccgctggc ggtttccagc ctcgaagaac tggcgaacgg
     8101 caccttcctg ccagccatcg gtgaaatcga cgagcttgat ccgaagggcg tgaagcgcgt
     8161 agtgatgtgt tctggtaagg tttattacga cctgctggaa cagcgtcgta agaacaatca
     8221 acacgatgtc gccattgtgc gtatcgagca actctacccg ttcccgcata aagcgatgca
     8281 ggaagtgttg cagcagtttg ctcacgtcaa ggattttgtc tggtgccagg aagagccgct
     8341 caaccagggc gcatggtact gcagccagca tcatttccgt gaagtgattc cgtttggggc
     8401 ttctctgcgt tatgcaggcc gcccggcctc cgcctctccg gcggtagggt atatgtccgt
     8461 tcaccagaaa cagcaacaag atctggttaa tgacgcgctg aacgtcgaat aaataaagga
     8521 tacacaatga gtagcgtaga tattctggtc cctgacctgc ctgaatccgt agccgatgcc
     8581 accgtcgcaa cctggcataa aaaacccggc gacgcagtcg tacgtgatga agtgctggta
     8641 gaaatcgaaa ctgacaaagt ggtactggaa gtaccggcat cagcagacgg cattctggat
     8701 gcggttctgg aagatgaagg tacaacggta acgtctcgtc agatccttgg tcgcctgcgt
     8761 gaaggcaaca gcgccggtaa agaaaccagc gccaaatctg aagagaaagc gtccactccg
     8821 gcgcaacgcc agcaggcgtc tctggaagag caaaacaacg atgcgttaag cccggcgatc
     8881 cgtcgcctgc tggctgaaca caatctcgac gccagcgcca ttaaaggcac cggtgtgggt
     8941 ggtcgtctga ctcgtgaaga tgtggaaaaa catctggcga aagccccggc gaaagagtct
     9001 gctccggcag cggctgctcc ggcggcgcaa ccggctctgg ctgcacgtag tgaaaaacgt
     9061 gtcccgatga ctcgcctgcg taagcgtgtg gcagagcgtc tgctggaagc gaaaaactcc
     9121 accgccatgc tgaccacgtt caacgaagtc aacatgaagc cgattatgga tctgcgtaag
     9181 cagtacggtg aagcgtttga aaaacgccac ggcatccgtc tgggctttat gtccttctac
     9241 gtgaaagcgg tggttgaagc cctgaaacgt tacccggaag tgaacgcttc tatcgacggc
     9301 gatgacgtgg tttaccacaa ctatttcgac gtcagcatgg cggtttctac gccgcgcggc
     9361 ctggtgacgc cggttctgcg tgatgtcgat accctcggca tggcagacat cgagaagaaa
     9421 atcaaagagc tggcagtcaa aggccgtgac ggcaagctga ccgttgaaga tctgaccggt
     9481 ggtaacttca ccatcaccaa cggtggtgtg ttcggttccc tgatgtctac gccgatcatc
     9541 aacccgccgc agagcgcaat tctgggtatg cacgctatca aagatcgtcc gatggcggtg
     9601 aatggtcagg ttgagatcct gccgatgatg tacctggcgc tgtcctacga tcaccgtctg
     9661 atcgatggtc gcgaatccgt gggcttcctg gtaacgatca aagagttgct ggaagatccg
     9721 acgcgtctgc tgctggacgt gtagtagttt aagtttcacc tgcactgtag accggataag
     9781 gcattatcgc cttctccggc aattgaagcc tgatgcgacg ctgacgcgtc ttatcaggcc
     9841 tacgggacca ccaatgtagg tcggataagg cgctagcgcc gcatccgaca agcgatgcct
     9901 gatgtgacgg ttaacgtgtc ttatcaggcc tacggggacc accaatgtag gtcggataag
     9961 gcgcaagcgc cgcatccgac aagcgatgcc tgatgtgacg tttaacgtgt cttatcaggc
    10021 ctacgggtga ccgacaatgc ccggaagcga tacgaaatat tcggtctacg gtttaaaaga
    10081 taacgattac tgaaggatgg acagaacaca tgaacttaca tgaatatcag gcaaaacaac
    10141 tttttgcccg ctatggctta ccagcaccgg tgggttatgc ctgtactact ccgcgcgaag
    10201 cagaagaagc cgcttcaaaa atcggtgccg gtccgtgggt agtgaaatgt caggttcacg
    10261 ctggtggccg cggtaaagcg ggcggtgtga aagttgtaaa cagcaaagaa gacatccgtg
    10321 cttttgcaga aaactggctg ggcaagcgtc tggtaacgta tcaaacagat gccaatggcc
    10381 aaccggttaa ccagattctg gttgaagcag cgaccgatat cgctaaagag ctgtatctcg
    10441 gtgccgttgt tgaccgtagt tcccgtcgtg tggtctttat ggcctccacc gaaggcggcg
    10501 tggaaatcga aaaagtggcg gaagaaactc cgcacctgat ccataaagtt gcgcttgatc
    10561 cgctgactgg cccgatgccg tatcagggac gcgagctggc gttcaaactg ggtctggaag
    10621 gtaaactggt tcagcagttc accaaaatct tcatgggcct ggcgaccatt ttcctggagc
    10681 gcgacctggc gttgatcgaa atcaacccgc tggtcatcac caaacagggc gatctgattt
    10741 gcctcgacgg caaactgggc gctgacggca acgcactgtt ccgccagcct gatctgcgcg
    10801 aaatgcgtga ccagtcgcag gaagatccgc gtgaagcaca ggctgcacag tgggaactga
    10861 actacgttgc gctggacggt aacatcggtt gtatggttaa cggcgcaggt ctggcgatgg
    10921 gtacgatgga catcgttaaa ctgcacggcg gcgaaccggc taacttcctt gacgttggcg
    10981 gcggcgcaac caaagaacgt gtaaccgaag cgttcaaaat catcctctct gacgacaaag
    11041 tgaaagccgt tctggttaac atcttcggcg gtatcgttcg ttgcgacctg atcgctgacg
    11101 gtatcatcgg cgcggtagca gaagtgggtg ttaacgtacc ggtcgtggta cgtctggaag
    11161 gtaacaacgc cgaactcggc gcgaagaaac tggctgacag cggcctgaat attattgcag
    11221 caaaaggtct gacggatgca gctcagcagg ttgttgccgc agtggagggg aaataatgtc
    11281 cattttaatc gataaaaaca ccaaggttat ctgccagggc tttaccggta gccaggggac
    11341 tttccactca gaacaggcca ttgcatacgg cactaaaatg gttggcggcg taaccccagg
    11401 taaaggcggc accacccacc tcggcctgcc ggtgttcaac accgtgcgtg aagccgttgc
    11461 tgccactggc gctaccgctt ctgttatcta cgtaccagca ccgttctgca aagactccat
    11521 tctggaagcc atcgacgcag gcatcaaact gattatcacc atcactgaag gcatcccgac
    11581 gctggatatg ctgaccgtga aagtgaagct ggatgaagca ggcgttcgta tgatcggccc
    11641 gaactgccca ggcgttatca ctccgggtga atgcaaaatc ggtatccagc ctggtcacat
    11701 tcacaaaccg ggtaaagtgg gtatcgtttc ccgttccggt acactgacct atgaagcggt
    11761 taaacagacc acggattacg gtttcggtca gtcgacctgt gtcggtatcg gcggtgaccc
    11821 gatcccgggc tctaacttta tcgacattct cgaaatgttc gaaaaagatc cgcagaccga
    11881 agcgatcgtg atgatcggtg agatcggcgg tagcgctgaa gaagaagcag ctgcgtacat
    11941 caaagagcac gttaccaagc cagttgtggg ttacatcgct ggtgtgactg cgccgaaagg
    12001 caaacgtatg ggccacgcgg gtgccatcat tgccggtggg aaagggactg cggatgagaa
    12061 attcgctgct ctggaagccg caggcgtgaa aaccgttcgc agcctggcgg atatcggtga
    12121 agcactgaaa actgttctga aataaatatc tgtaataaga aatagccctc gccgcttccc
    12181 tctacaggaa tggcgaaggg ctgtcggttt cgacatggtt ggccatcgta tgatggcctt
    12241 ttttgtgctt atcgcgatga ttttcgctgc gctatcaggg taaatttata gtcatcggta
    12301 ttaaaagcgt tgcggctata ttcaaacacc cgaccatcaa ctaaatatcc acgcgatact
    12361 ttttcaagaa tcggctttgt ctggctgata ttaagcagac ggctcatctc ttcggttggc
    12421 atcagaggaa tgatttcctg ttcgctacga tcgataacca ttttcttcac ttcttcgata
    12481 aagtgatatt tcgaattttc catgacctgc caggtgagat ccgggaacaa cgcaagcggc
    12541 atccaggttt cttccagcgc cattggcttt tgcttgcgat agcgcacgcg cttcacatgc
    12601 cacacacgat cctgcggggt gatttgtagc tgttgctgaa gaaaatcgtc agccggaatc
    12661 acttcgaata tcagaacttc actgtgtgta tcgacgtgac ggtccgacag tttttcatca
    12721 aaactggtta actgaaaaat atcgtaattg acccgctctt ctttgacgta agtcccgctg
    12781 ccctgaatgc tttcgaggat ctgctgctcg actagctggc gcaaagcctg acgcaccgta
    12841 acccggctga cgccaaactc tgtttgtagc gctgattcag tgggtaacgc atcgccaggt
    12901 ttaagctcgc cacgcgcaat ttgttcacga atgcgatcgg caatctgccg gtataagggc
    12961 ttgtgtccca tttttagtat ctcattaata cgaatttaac cattatgccc gataaattca
    13021 tcctgtaaat aatacaaata caatacaaat aatttcaatc aagtgaaatt gatcacataa
    13081 tggtattgtt ttatcgggca cactggcgcg actataaaaa cgatcaagtg aggatcatga
    13141 tgaatctgac gactctgacc caccgcgatg cgttgtgtct gaatgcgcgc tttaccagcc
    13201 gtgaagaggc catccacgcg ttgactcaac gtcttgctgc tctggggaaa atttccagta
    13261 ctgagcaatt tctggaagaa gtgtatcgcc gtgaaagcct tggcccgacc gccttaggtg
    13321 aagggttggc tgtgccgcat ggcaaaactg ctgcggtaaa agaagcggcg tttgcggtcg
    13381 ccacactcag cgagccgctt cagtgggaag gcgttgatgg cccggaagca gttgatttag
    13441 tggtgctgct ggcgattccc cccaatgaag cgggtactac gcatatgcaa ctgctgacag
    13501 cgctgaccac gcgccttgcg gatgatgaga ttcgggcgcg tatacagtcg gcgacgacgc
    13561 ctgatgagtt gctctcggcg ctggatgaca agggaggcac gcaaccttct gcctcttttt
    13621 ccaacgcgcc aactatcgtc tgcgtaacgg cctgtccggc gggtattgct cacacctata
    13681 tggctgcgga atatctggaa aaagccggac gcaaactcgg cgtaaatgtt tacgttgaaa
    13741 aacaaggcgc taacggcatt gaagggcgtt taacggcgga tcaactcaat agtgcaaccg
    13801 cctgtatttt tgcggctgaa gtcgccatca aggagagtga gcgttttaat ggcattcccg
    13861 cgctttcagt gcctgttgcc gagccgattc gccatgcaga agcgttgatc caacaagcgc
    13921 ttaccctcaa gcgtagcgat gagacgcgta ccgtacagca agatacgcaa ccggtgaaaa
    13981 gtgtcaaaac ggagctgaaa caggcactgt tgagcggaat ctcttttgcc gtaccgttga
    14041 ttgtcgcggg gggcacggtg ctggcggtcg cggtattact gtcgcaaatc ttcgggctac
    14101 aagatctgtt taatgaagaa aactcctggc tgtggatgta ccgcaagctg ggcggcgggc
    14161 tgctcggaat tttgatggta ccggtgctcg cggcctatac cgcctattct ctggcagata
    14221 aaccggcgtt agcgccaggc tttgcggctg gacttgccgc caacatgatc ggctccgggt
    14281 ttctcggcgc ggtcgttggc ggattgatag ccggttactt gatgcgctgg gtgaaaaatc
    14341 acttgcgtct tagcagtaaa ttcaatggat tcctgacttt ttatctctac ccggtgctcg
    14401 gtacgttggg agcgggcagt ctgatgctgt ttgtggtggg ggaacctgtc gcctggatca
    14461 ataactcgct taccgcctgg ctgaacggtc tgtcaggaag taacgcgctg ttgctgggtg
    14521 ccattctcgg ttttatgtgt tcctttgacc ttggagggcc agtgaataaa gccgcttatg
    14581 cattctgcct gggcgcaatg gcgaacggcg tttacggccc gtatgccatt ttcgcctccg
    14641 tcaaaatggt ttcggcattt accgtaaccg cttccacgat gctcgcaccg cgcctgttta
    14701 aagagtttga aattgagacc gggaaatcca cctggctgtt agggctggca ggtattaccg
    14761 aaggggcgat cccgatggcg attgaagatc cgctgcgggt tattggttcg tttgtgctgg
    14821 gctctatggt aacgggcgct attgtcggtg cgatgaatat cggcctttcg acacccggtg
    14881 ccggcatttt ctcgctcttt ttacttcatg ataatggcgc gggcggtgtt atggcggcaa
    14941 ttggctggtt tggcgcggca ttggtggggg ctgcaatctc gactgcaatt ctcctgatgt
    15001 ggcggcgtca cgcggttaag catggcaact atctgactga tggcgtaatg ccataaacaa
    15061 aaaggaaacg acgatgaaag cagtatctcg cgttcacatc accccgcata tgcactggga
    15121 tcgagagtgg tatttcacca ccgaagagtc acgtattctg ctggtcaata atatggaaga
    15181 gatcctgtgc cgactggaac aggacaacga atacaaatat tacgtactcg acgggcaaac
    15241 ggcgatcctc gaagattatt tcgcggtgaa accggaaaac aaagaccgtg tgaagaaaca
    15301 ggtagaagcc ggcaagttga ttatcggccc ctggtatacc cagaccgata ccacgattgt
    15361 ttctgcggaa tccatcgtcc gtaatctgat gtacggaatg cgtgactgcc tcgcgtttgg
    15421 cgagccgatg aaaataggtt atttaccaga ttcctttggc atgtccgggc aactgccgca
    15481 tatctacaat ggatttggca ttacccgcac catgttctgg cgcggatgtt cggagcgcca
    15541 cggtactgat aaaaccgagt ttttgtggca aagcagtgac ggtagcgaag tgacggcgca
    15601 ggtgctgccg ctgggctacg ccatcggtaa gtacttacct gccgacgaaa acggattacg
    15661 taaacggctc gacagttatt ttgacgtgct ggaaaaagcg tctgtaacca aagagatttt
    15721 gctgccgaat gggcatgacc agatgccatt gcagcaaaat atcttcgaag tgatggataa
    15781 gctacgtgag atctaccctc aacgtaagtt tgtgatgagc cgctttgaag aggtatttga
    15841 gaagatcgaa gcgcagcgag ataatctggc aaccctgaaa ggggaattta ttgatggcaa
    15901 atatatgcgc gtgcatcgca ccatcggttc tacgcgtatg gatatcaaaa ttgcccacgc
    15961 gcgtattgaa aataagattg ttaatctgct ggaaccgctg gcaacactgg cctggacgtt
    16021 gggttttgaa taccaccacg gcttgctgga gaaaatgtgg aaagagatct taaaaaatca
    16081 tgcccacgac agtatcggct gctgctgtag tgacaaagtt catcgcgaaa tcgtcgcccg
    16141 cttcgaactg gctgaagaca tggcggataa tctgattcgt ttctacatgc gcaaaattgc
    16201 cgacaacatg ccgcagagcg acgccgacaa actcgtcctg tttaacctga tgccctggcc
    16261 gcgtgaagaa gttatcaaca ccactgtgcg gctgcgcgcc agccagttta atttgcggga
    16321 cgatcgcggt cagcctgtac cgtattttat tcgccatgcc cgtgagatcg atccaggcct
    16381 aatcgatcgg caaatagttc attacggtaa ttacgatccc tttatggagt ttgatataca
    16441 gatcaaccag attgtccctt ctatgggcta tcgcacgctt tatatcgaag cgaatcagcc
    16501 tggcaacgta attgcggcaa aaagtgacgc tgaagggata ctggaaaatg ctttctggca
    16561 aattgcgctc aatgaggatg gttctctgca actggtagat aaagacagcg gtgtgcgcta
    16621 tgaccgggta ttgcaaattg aagaaagctc tgatgatggt gatgaatatg actattcacc
    16681 cgcaaaagaa gagtgggtaa ttaccgcagc gaacgcgaaa ccgcaatgcg atattattca
    16741 tgaagcctgg cagagcaggg ctgttatccg ctatgacatg gcagtgccgc tcaatttgtc
    16801 agaacgcagc gcccggcaat ccactggcag agtaggggtg gtgttggttg tcactcttag
    16861 tcataacagc aggcgtattg atgtggatat caatcttgat aaccaggctg acgatcatcg
    16921 ccttcgtgtc ctggtcccta caccttttaa caccgacagt gttctggcag atacgcagtt
    16981 tggttcgcta acgcgccccg tgaacgacag tgcaatgaac aactggcagc aagaaggctg
    17041 gaaagaagcg ccggttccgg tatggaatat gctcaactat gttgccttac aggaagggcg
    17101 taacggcatg gctgtcttta gcgaagggtt acgtgaattt gaagtcatcg gtgaagagaa
    17161 gaaaaccttt gccattacgt tgctgcgtgg cgtgggctta ctgggcaaag aagatctgct
    17221 tttaaggcct gggcggcctt cgggaattaa aatgccagtc ccggactcac aactacgtgg
    17281 tctgctttct tgtcgcctaa gtttattgag ttataccggt acgccaaccg ccgctggtgt
    17341 agctcagcag gcgcgagcat ggctgactcc agtacagtgt tacaacaaaa tcccatggga
    17401 tgtgatgaag ctcaacaaag ccggattcaa cgtgccggaa agttatagtt tgttgaaaat
    17461 gcccccagtg ggatgcctga taagcgcact taagaaagct gaagaccgac aagaagtgat
    17521 tttacggctg tttaatccgg ctgaatcagc aacctgtgat gcgactgttg ctttcagtcg
    17581 cgaggtgatt tcttgctcag aaacgatgat ggatgaacac attaccaccg aggaaaatca
    17641 aggttcaaat ctatcggggc cttttttacc cggccagtca cggacgttca gttaccggct
    17701 tgcctgaata gcaatcaaac cgaagccaca tatgcgcggc cagattgttg acaaagggcg
    17761 ctttgttcat gccggatacg gcatgaacgc tttattcggt ctacaaaagc aggcaaattc
    17821 aatatattgc agagatgatg tagacactgg caagcgtagc gcatcaggta attttgcgtt
    17881 tatcttcact ctcaagccac gtatatgtgg ctttattttt aacaaaataa taaccctggg
    17941 tgagttaatt ataatataat tataagttaa ctaaatgtta atattggcgg ggtggattta
    18001 tgcctttatt agtaatcctg aaactctgcg tcgtattagc cagtgaccaa aaaaagaatt
    18061 aaggtcaacc gtgctgtttt tgcttcgtct ctttttatct ttaattgcca accgaaacta
    18121 atttcagcct tataactcac acattttaaa cataaatgtc actaaagtta ccttattgaa
    18181 acatgattaa cataatttgt aggaattgat atttatcaat gtataagtct tggaaatggg
    18241 catcaaaaag agataaattg ttctcgatca aattggctga aaggcggtaa tttagctata
    18301 aattgatcac cgtcgaaaaa tgcaaatttg cttcaacaaa aacctgttta ttgtaaggat
    18361 tttgcggcgt aatatatacg tgggatcaat ttgagttttt attaacatgt ttgcaacctt
    18421 tctttacgcc gtttttgtgt gcattcacat ggtatgatga aagtgttcaa acaaatttct
    18481 attggggcat gcgtgtgacc ctttctaacg gggttcactc tcggagtctt catgcgatga
    18541 gcaaggagtc atgatgttag atatagtcga actgtcgcgc ttacagtttg ccttgaccgc
    18601 gatgtaccac ttcctttttg tgccactgac gctcggtatg gcgttcctgc tggccattat
    18661 ggaaacggtc tacgtcctct ccggcaaaca gatttataaa gatatgacca agttctgggg
    18721 caagttgttt ggtatcaact tcgctctggg tgtggctacc ggtctgacca tggagttcca
    18781 gttcgggact aactggtctt actattccca ctatgtaggg gatatcttcg gtgcgccgct
    18841 ggcaatcgaa ggtctgatgg ccttcttcct cgaatccacc tttgtaggtc tgttcttctt
    18901 cggttgggat cgtctgggta aagttcagca tatgtgtgtc acctggctgg tggcgctcgg
    18961 ttcaaacctg tccgcactgt ggattctggt tgcgaacggc tggatgcaaa acccaatcgc
    19021 gtccgatttc aactttgaaa ctatgcgtat ggagatggtg agcttctccg agctggtgct
    19081 taacccggtt gctcaggtga aattcgttca cactgtagcg tctggttatg tgactggcgc
    19141 gatgttcatc ctcggtatca gcgcatggta tatgctgaaa ggtcgtgact tcgccttcgc
    19201 taaacgctcc tttgctatcg ctgccagctt cggtatggct gctgttctgt ctgttattgt
    19261 tctgggtgat gaatccggct acgaaatggg cgacgtgcag aaaaccaaac tggctgctat
    19321 tgaagccgag tgggaaacgc aacctgcgcc tgctgccttt actctgttcg gcattcctga
    19381 tcaggaagag gagacgaaca aatttgcgat tcagatccct tacgcactgg gcatcattgc
    19441 aacgcgttcc gtggataccc cggttatcgg cctgaaagag ctgatggtgc agcatgaaga
    19501 acgcattcgt aacgggatga aggcgtactc tctgctcgaa caactgcgtt ctggttctac
    19561 cgaccaggcg gttcgtgacc agttcaatag catgaagaaa gacctcggtt acggtctgct
    19621 gctgaaacgc tatacgccaa acgtggctga tgcgactgaa gcgcagattc aacaggcaac
    19681 caaagactcc atcccgcgtg tagcgccgct gtactttgcg ttccgtatca tggtggcgtg
    19741 tggcttcctg cttctggcaa tcatcgcgct ctctttctgg agtgtcatcc gcaaccgcat
    19801 tggcgagaaa aaatggcttc tgcgcgccgc gctgtacggt attccgctgc cgtggattgc
    19861 tgtagaagcg ggctggttcg tggctgaata tggccgccaa ccgtgggcta tcggtgaagt
    19921 gctgccgaca gctgtggcga actcgtcact gaccgcaggc gatctcatct tctcaatggt
    19981 gctgatttgc ggcctgtata ccctgttcct ggtggcagaa ttgttcttaa tgttcaagtt
    20041 tgcacgcctc ggcccaagca gcctgaaaac cggtcgctat cactttgagc agtcttccac
    20101 gactactcag ccggcacgct aagacaggag tcgtcaaatg atcgattatg aagtattgcg
    20161 ttttatctgg tggctgctgg ttggcgttct gctgattggt tttgcagtca ctgacggttt
    20221 cgacatgggg gtgggcatgc tcacccgttt cctcggtcgt aacgacaccg agcgtcgaat
    20281 tatgattaac tccattgcac cacactggga cggtaaccag gtttggctga tcaccgcggg
    20341 cggcgcactc tttgctgcct ggccgatggt ctatgccgct gcgttctccg gcttctatgt
    20401 ggcgatgatc ctcgtgctgg cgtctttgtt cttccgtccg gtcggttttg actaccgctc
    20461 caagattgaa gaaacccgct ggcgtaacat gtgggactgg ggcatcttca ttggtagctt
    20521 cgttccgccg ctggtaattg gtgtagcgtt cggtaacctg ttgcagggcg taccgttcaa
    20581 cgttgatgaa tatctgcgtc tgtactacac cggtaacttc ttccagttgc ttaacccgtt
    20641 cggcctgctg gcaggcgtgg tgagcgtagg gatgatcatt actcagggcg caacctatct
    20701 gcaaatgcgt accgtgggcg aactgcacct gcgtacccgt gcaacggctc aggtggctgc
    20761 gctggtgaca ctggtctgtt tcgcactggc tggcgtatgg gtgatgtacg gtatcgatgg
    20821 ttatgtcgtg aaatcgacaa tggaccatta cgcagcctct aacccactga ataaagaagt
    20881 ggttcgtgaa gctggcgcat ggctggttaa cttcaacaac acgccaattc tgtgggctat
    20941 tccggcactg ggtgtggttc tgccgctgct gaccatcctg actgcacgta tggataaagc
    21001 cgcgtgggcg tttgtgttct cctccctgac gctggcctgc atcatcctga cagccggtat
    21061 cgcaatgttc ccgtttgtga tgccgtccag caccatgatg aacgcaagtc tgacaatgtg
    21121 ggatgcaact tccagccagc tgacgcttaa cgtcatgacc tgggttgcgg tggttctggt
    21181 accgatcatt ctgctctaca ccgcctggtg ttactggaaa atgttcggtc gtatcaccaa
    21241 agaagatatt gaacgtaaca cccactctct gtactaagta aggagctaaa aatgtggtat
    21301 ttcgcatgga ttctgggaac gcttcttgcc tgttcgtttg gggtaatcac cgcgctggcg
    21361 cttgaacacg tcgaatcagg caaagccggt caagaagaca tctgatgagt aagattatcg
    21421 cgactttgta tgcggtaatg gacaagcgcc ccctgcgggc gctttccttc gtgatggcgc
    21481 ttctgttagc aggatgtatg ttttgggacc catctcgttt tgccgcgaag accagtgaac
    21541 tggaaatctg gcatggttta ttgctgatgt gggccgtctg tgctggtgtg attcacggcg
    21601 tgggctttcg tccgcagaag gttctttggc aagggatttt ttgcccattg cttgccgata
    21661 ttgttctcat tgtcgggctg attttcttct tcttttaaat cagaattctc tttaaaaaat
    21721 tatgggccgc tccaggccca taaattttta cgctccctta acttgccctc attcccaaac
    21781 ctcaatcgcg cgcgtatagt agcagcgttt aaaagctcta acttttgttg cattaccggg
    21841 atgtaaagtg aatacaacgc tgtttcgatg gccggttcgc gtctactatg aagataccga
    21901 tgccggtggt gtggtgtacc acgccagtta cgtcgctttt tatgaaagag cacgcacaga
    21961 gatgctgcgt catcatcact tcagtcagca ggcgctgatg gctgaacgcg ttgcctttgt
    22021 ggtacgtaaa atgacggtgg aatattacgc acctgcgcgg ctcgacgata tgctcgaaat
    22081 acagactgaa ataacatcaa tgcgtggcac ctctttggtt ttcacgcaac gtattgtcaa
    22141 cgccgagaat actttgctga atgaagcaga ggttctggtt gtttgcgttg acccactcaa
    22201 aatgaagcct cgtgcgcttc ccaagtctat tgtcgcggag tttaagcagt gactgacatg
    22261 aatatccttg atttgttcct gaaggctagc cttctggtta aacttatcat gttgattttg
    22321 attggttttt caatcgcatc ttgggccatt attatccagc ggacccgtat tcttaacgca
    22381 gcggcgagcg aagccgaagc gtttgaagat aaattctggt ctggaatcga actctctcgc
    22441 ctctatcaag agagccaggg gaaacgggat aatctgactg gttcggaaca aatcttttac
    22501 agcgggttca aagagtttgt gcgcctgcat cgtgccaata gccatgcgcc ggaagccgta
    22561 gtggaagggg cgtcgcgtgc tatgcgtatc tccatgaacc gtgaacttga aaatctggaa
    22621 acgcacattc cgttcctcgg tacggttggc tccatcagcc cgtatattgg tctgtttggt
    22681 acggtctggg ggatcatgca cgcctttatc gccctcgggg cggtaaaaca agcaacactg
    22741 caaatggttg cgcccggtat cgcagaagcg ttgattgcga ctgcaattgg tctgtttgcc
    22801 gctatcccgg cagttatggc ctacaaccgc ctcaaccagc gcgtaaacaa actggaactg
    22861 aattacgaca actttatgga agagtttacc gcgattctgc accgccaggc gtttaccgtt
    22921 agcgagagca acaaggggta agccatggcc agagcgcgtg gacgaggtcg tcgcgatctc
    22981 aagtccgaaa tcaacattgt accgttgctg gacgtactgc tggtgctgtt gctgatcttt
    23041 atggcgacag cgcccatcat cacccagagc gtggaggtcg atctgccaga cgctactgaa
    23101 tcacaggcgg tgagcagtaa cgataatccg ccagtgattg ttgaagtgtc tggtattggt
    23161 cagtacaccg tggtggttga gaaagatcgc ctggagcgtt taccaccaga gcaggtggtg
    23221 gcggaagtgt ccagccgttt caaggccaac ccgaaaacgg tctttctgat cggtggcgca
    23281 aaagatgtgc cttacgatga aataattaaa gcactgaact tgttacatag tgcgggtgtg
    23341 aaatcggttg gtttaatgac gcagcctatc taaacatctg cgtttccctt gcttgaaaga
    23401 gagcgggtaa caggcgaaca gtttttggaa accgagagtg tcaaaggcaa ccgaacaaaa
    23461 cgacaagctc aagcgggcga taattatttc agcagtgctg catgtcatct tatttgcggc
    23521 gctgatctgg agttcgttcg atgagaatat agaagcttca gccggaggcg gcggtggttc
    23581 gtccatcgac gctgtcatgg ttgattcagg tgcggtagtt gagcagtaca aacgcatgca
    23641 aagccaggaa tcaagcgcga agcgttctga tgaacagcgc aagatgaagg aacagcaggc
    23701 tgctgaagaa ctgcgtgaga aacaagcggc tgaacaggaa cgcctgaagc aacttgagaa
    23761 agagcggtta gcggctcagg agcagaaaaa gcaggctgaa gaagccgcaa aacaggccga
    23821 gttaaagcag aagcaagctg aagaggcggc agcgaaagcg gctgcagacg caaagaaaaa
    23881 agcagaagca gaagccgcca aagccgtagc cgaagcgcag aaaaaagccg aggcagccgc
    23941 tgcggcactg aagaagaaag cggaagcggc agaagcagct gcagctgaag caagaaagaa
    24001 agcggcaact gaagctgctg aaaaagccaa agcagaagct gagaagaaag cggctgctga
    24061 aaaggctgca gctgataaga aagcggcagc agagaaagct gcagccgaca aaaaagcagc
    24121 agaaaaagcg gctgctgaaa aggcagcagc tgataagaaa gcagcggcag aaaaagccgc
    24181 cgcagacaaa aaagcggcag cggcaaaagc tgcagctgaa aaagccgctg cagcaaaagc
    24241 ggccgcagag gcagatgata ttttcggtga gctaagctct ggtaagaatg caccgaaaac
    24301 ggggggaggg gcgaaaggga acaatgcttc gcctgccggg agtggtaata ctaaaaacaa
    24361 tggcgcatca ggggccgata tcaataacta tgccgggcag attaaatctg ctatcgaaag
    24421 taagttctat gacgcatcgt cctatgcagg caaaacctgt acgctgcgca taaaactggc
    24481 acccgatggt atgttactgg atatcaaacc tgaaggtggc gatcccgcac tttgtcaggc
    24541 tgcgttggca gcagctaaac ttgcgaagat cccgaaacca ccaagccagg cagtatatga
    24601 agtgttcaaa aacgcgccat tggacttcaa accgtaatcg cgatgttgac tgttcggacg
    24661 gtcaacatca ggcaccggtt gccacggggt tctggtagtt ttgtgtattt tagtttgtta
    24721 acattctgct aaattatcgt gggccatcgg tccagataag ggagatatga tgaagcaggc
    24781 attacgagta gcatttggtt ttctcatact gtgggcatca gttctgcatg ctgaagtccg
    24841 cattgtgatc gacagcggtg tagattccgg tcgtcctatt ggtgttgttc ctttccagtg
    24901 ggcggggcct ggtgcggcac ctgaagatat tggcggcatc gttgctgctg acttgcgtaa
    24961 cagcggtaaa tttaatccgt tagatcgcgc tcgtctgcca cagcagccgg gtagtgcgca
    25021 ggaagtacaa ccagctgcat ggtccgcact gggcattgac gctgtagttg tcggtcaggt
    25081 cactccgaat ccggatggtt cttacaatgt tgcttatcaa cttgttgaca ctggcggcgc
    25141 accgggtact gtacttgctc agaactcgta caaagtgaac aagcagtggc tgcgttatgc
    25201 tggtcatacc gccagtgatg aagtgtttga aaaactgacc ggcattaaag gtgcgttccg
    25261 tactcgtatt gcctacgttg ttcagaccaa cggcggtcag ttcccgtatg aactgcgcgt
    25321 atctgactat gacggttaca accagtttgt cgttcaccgt tcaccgcagc cgctgatgtc
    25381 tccggcgtgg tcaccagacg gttctaaact ggcttatgtg accttcgaaa gcggtcgttc
    25441 cgcgctggtt attcagacgc tggcaaatgg cgctgtacgt caggtggctt cattcccgcg
    25501 tcacaacggt gcacctgcat tctcgccaga cggcagcaaa ctggcattcg ccttgtcgaa
    25561 aaccggtagt ctgaacctgt acgtaatgga tttggcttct ggtcagatcc gccaggtgac
    25621 tgatggtcgc agtaacaata ccgaaccgac ctggttcccg gatagccaga acctggcatt
    25681 tacttctgac caggccggtc gtccgcaggt ttataaagtg aatatcaacg gcggtgcgcc
    25741 acaacgtatt acctgggaag gttcgcagaa ccaggatgcg gatgtcagca gcgacggtaa
    25801 atttatggta atggtcagct ccaatggtgg gcagcagcac attgccaaac aagatctggc
    25861 aacgggaggc gtacaagttc tgtcgtccac gttcctggat gaaacgccaa gtctggcacc
    25921 taacggcact atggtaatct acagctcttc tcaggggatg ggatccgtgc tgaatttggt
    25981 ttctacagat gggcgtttca aagcgcgtct tccggcaact gatggacagg tcaaatcccc
    26041 tgcctggtcg ccgtatctgt gataataatt aattgaatag taaaggaatc attgaaatgc
    26101 aactgaacaa agtgctgaaa gggctgatga ttgctctgcc tgttatggca attgcggcat
    26161 gttcttccaa caagaacgcc agcaatgacg gcagcgaagg catgctgggt gccggcactg
    26221 gtatggatgc gaacggcggc aacggcaaca tgtcttccga agagcaggct cgtctgcaaa
    26281 tgcaacagct gcagcagaac aacatcgttt acttcgatct ggacaagtac gatatccgtt
    26341 ctgacttcgc tcaaatgctg gatgcacatg caaacttcct gcgtagcaac ccgtcttaca
    26401 aagtcaccgt agaaggtcac gcggacgaac gtggtactcc ggaatacaac atctccctgg
    26461 gtgaacgtcg tgcgaacgcc gttaagatgt acctgcaggg taaaggcgtt tctgcagacc
    26521 agatctccat cgtttcttac ggtaaagaaa aacctgcagt actgggtcat gacgaagcgg
    26581 catactccaa aaaccgtcgt gcggtactgg tttactaaga gaattgcatg agcagtaact
    26641 tcagacatca actattgagt ctgtcgttac tggttggtat agcggccccc tgggccgctt
    26701 ttgctcaggc accaatcagt agtgtcggct caggctcggt cgaagaccgc gtcactcaac
    26761 ttgagcgtat ttctaatgct cacagccagc ttttaaccca actccagcaa caactttctg
    26821 ataatcaatc cgatattgat tccctgcgtg gtcagattca ggaaaatcag tatcaactga
    26881 atcaggtcgt ggagcggcaa aagcagatcc tgttgcagat cgacagcctc agcagcggtg
    26941 gtgcagcggc gcaatcaacc agcggcgatc aaagcggtgc ggcggcatca acgacgccga
    27001 cagctgatgc tggtactgcg aatgctggcg cgccggtgaa aagcggtaat gcaaacacgg
    27061 attacaatgc agctattgcg ctggtgcagg ataaatcccg ccaggatgac gcaatggtgg
    27121 catttcagaa tttcatcaaa aattaccctg attcaactta cctgccaaac gccaattatt
    27181 ggctgggtca gttaaactac aacaagggta aaaaagatga tgcggcgtac tattttgctt
    27241 cggtagtgaa aaactatccg aagtcaccaa aggctgcaga tgcgatgttt aaagtcggcg
    27301 tcatcatgca ggacaaaggt gacaccgcaa aagcgaaagc cgtataccag caggttatca
    27361 gtaaataccc tggtactgat ggcgctaaac aggcacaaaa acgtctgaac gcgatgtaat
    27421 gcataacaca cgaccagaag tcgcattatt tctggtcgtg tcgtgcgaat cataagcagt
    27481 tgagtgatct acatcgaaat ttttgttgcg ctcaagtctg aaatcagtaa tatatgccgc
    27541 cgttgccacg ggatatcaaa caaaccgaaa gcaacgaaaa agtgggtcgt tagctcagtt
    27601 ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccaa
    27661 tcgctaaggt ggaagcggta gtaaaacgtg aaggataacg ttgcatgagc aacggcccga
    27721 agggcgagac gaagtcgagt catcctgcac gacccaccac taacatagtt agttgtagta
    27781 tccagcgtag tatcgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg
    27841 tcggcggttc gatcccgtca tcacccacca ccgggtcgtt agctcagttg gtagagcagt
    27901 tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccagt tttaacatcg
    27961 aagacagatg ttaagcgtgt aggataacgt tgcgtcagca acggcccgta gggcgagcga
    28021 agcgagtcat cctgcacgac ccaccactaa tgacggtggg ttcggtggaa gtagtttgta
    28081 gtatccagcg cagtatcggg tgattagctc agctgggaga gcacctccct tacaaggagg
    28141 gggtcggcgg ttcgatcccg tcatcaccca ccact
//
LOCUS       DBBGLQ010000055        28045 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.055, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000055 DBBGLQ010000000
VERSION     DBBGLQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.055"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(83..376)
                     /locus_tag="ACIYSB_003205"
     CDS             complement(83..376)
                     /locus_tag="ACIYSB_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430192.1"
                     /translation="MSDYIYMTITSVKSITDNSPFWSYDLGYYLYSGKLVSFYCEGMS
                     NEWDMCSAIADHFDVVGDFSFDVIRKKKRTLIRGNCVFLFEQPFPGAYKIPLY"
     gene            complement(417..1322)
                     /locus_tag="ACIYSB_003206"
     CDS             complement(417..1322)
                     /locus_tag="ACIYSB_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HGC5430193.1"
                     /translation="MSRREKLLKKDLVAHVRKEKGSFKKLSDYTRIMHRFAETLARLN
                     VQISSAAQLKVRHIELYMNSRTDVSKRTRENEMSAIRAVLRQVGKNKLADPSHPRLSN
                     KALGISGESRKGTKSLITDDRFREILQRIEQKDKGLAATVKLSRYLGLRNEEAIQSAK
                     SLKTWKQALERGDEKLRVIFGTKGGRERMTTIVDRNNVIEAVNYAIKYAGEHDGRLID
                     RGNLKSAMNYYLNSLRRYGEMKGGDETPHSFRYSYAASAMKYHIMNGYSYHEALALTS
                     MDLGHGDGRGRYIEQVYCQLLGHEE"
     gene            2588..3154
                     /locus_tag="ACIYSB_003207"
     CDS             2588..3154
                     /locus_tag="ACIYSB_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430194.1"
                     /translation="MQSLLFIFLLLTGGYLSSHIFNTGSGKWLFITIAITFLLNTALI
                     FLFIFTKAYFFSQHVNKMREKAKQTNSLDFINCLIKLHKKYPVYVLYAPSENTVEICY
                     NIFNVNPVIGKKLYLKTLSNRHIRFTVKNIILLPALNDDFICTLESFYNNSDETKDII
                     DNYIRKIQGNQQLPWLINNAVPTDTKEK"
     gene            3250..3567
                     /locus_tag="ACIYSB_003208"
     CDS             3250..3567
                     /locus_tag="ACIYSB_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430195.1"
                     /translation="MNSTRKEKYSAFCRLLNETLKYELRLILPPGHQAVIPLLESPRG
                     EISRDTIEKMRDILTPDVTHRIKESINAWTEDELSYLDCTVDVEYVKEQKRKLFAMLD
                     CEQ"
     gene            complement(3898..4086)
                     /locus_tag="ACIYSB_003209"
     CDS             complement(3898..4086)
                     /locus_tag="ACIYSB_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430196.1"
                     /translation="MWILIFWLSTPYSVSSSVARQPTLAVHSQEFASERACRNALAEM
                     KKVNVGELTLRGICVSKE"
     gene            complement(4344..4607)
                     /locus_tag="ACIYSB_003210"
     CDS             complement(4344..4607)
                     /locus_tag="ACIYSB_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGC5430197.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYYKIGSTIKTERVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFNQRFGRFVDTKKGA"
     gene            complement(4832..5404)
                     /locus_tag="ACIYSB_003211"
     CDS             complement(4832..5404)
                     /locus_tag="ACIYSB_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430198.1"
                     /translation="MKISKLVVMATVCSTLSACSVEEMAAVNKKISDGAFAVTKALNG
                     GTGYADNGMPILNKQLPPEQDKRENIIDVPVDVDTAAGRLKRYYKFYSNDEVNAIRNN
                     GTTDGKWQASAIIDNGHIWDAMPGSYYKMGGNWRKGSFDDHLTIEVEKNGKGSRVYIT
                     YASASQAHLQASSLEPLFKRVKDVAEGKVR"
     gene            5701..6000
                     /locus_tag="ACIYSB_003212"
     CDS             5701..6000
                     /locus_tag="ACIYSB_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430199.1"
                     /translation="MTNETEKAMWSCKTSDTPWTFSRLLKKAICAVLPDSGPHTVHRV
                     NKKENNYLTSVLSDICDSNEKDSNEAMFNPFHPLYRDHHPIDSREWSGSPYKTPE"
     gene            complement(6047..7549)
                     /locus_tag="ACIYSB_003213"
     CDS             complement(6047..7549)
                     /locus_tag="ACIYSB_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HGC5430200.1"
                     /translation="MTTNNYLEHFLTLLGWLVNNGLWDLLTGTGLFALPLAFRVIGIW
                     LKVREEGADEGNKGMLSLPRIEHALYGAFFVMVACCVPLVQVTLDTLKFDRSRAQTCG
                     VWTPKAPGDTGYSGVISSLGDRTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDIRQLR
                     FEVQRTFIANRALADELQDFTNDCYSLALYQWKQRDQGMTKDRKLLSDISWIGSSTFM
                     QGDYGTLQSKMPRAAFPWNSARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVTEEVDE
                     GLWLRISAAMKMLGNAQANYQEEIIKRLVSPANLTLSQGGEVYRGYGGNADMTAANAV
                     TRAAGTAGSAVASLAAFPAFDAMRQALPMVQSILLMAVYIMLPLILAFAAYEFSTLIT
                     LTFVVFALNFLTFWWELARWLDSWMLTALYSSDTHSRVNMMGLQNTSDDIIMNFVMGT
                     MFLVLPAVWLGALSWAGVNIGQAISEGFNRAGHVTQQAGQHAGSFVKRIK"
     gene            complement(7546..7905)
                     /locus_tag="ACIYSB_003214"
     CDS             complement(7546..7905)
                     /locus_tag="ACIYSB_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430201.1"
                     /translation="MAMTQGTKAGLVLKGVKVAGMWLSGLIAMMILALLVADTGMRHT
                     QEVEALRLWLHETRFGWLGWRVCLYGALAWGCRKIWRAPGFRPEYRRPFARICLVSAL
                     FALLCEYSIFFGPGVEL"
     gene            complement(7910..9325)
                     /locus_tag="ACIYSB_003215"
     CDS             complement(7910..9325)
                     /locus_tag="ACIYSB_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HGC5430202.1"
                     /translation="MKMKTGVVALSVMLCCSTPVMADLLSLPKLNDSTFGYGKSVDGA
                     VSDKLFYTLGGGSVISQPATRSNMQKLGMDLGWSSDLMCGNFDLKTTVGNQLNGVTEG
                     FKNLMGDVIQGATGAVASLPAMVIQRANPGLYDMITNGVLQANVAFDKAQLNCQNMAK
                     RMMDFSDRSKWTQAAMLEEYKKVVNSGDGDAVKGDEAGRQATGKEGKKWIGGQQRGGA
                     GQPAIRPVHDLTAAGFNMMNGLPVTSTASVSAGNCNGSACEMFRNAEEAAKAVVKVLG
                     DRTMRTCTDAKACTSGDNEQQQGTVAGTGFAPVLEEATRVNTEQLVRLVNGQEKPTAE
                     NLAKLKTGSLAVSAGVINALRRDPDNMSLTSRLAGELAMAHTVETALVMRRMLLTGMS
                     EPYAAAEDAAIQEGDRRIASLDREIMALKNEMELKRDLARNSVLTIIERDNERVGSNP
                     MIQPADNTDSRVRALETPDGE"
     gene            complement(9338..10312)
                     /locus_tag="ACIYSB_003216"
     CDS             complement(9338..10312)
                     /locus_tag="ACIYSB_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HGC5430203.1"
                     /translation="MMSLKGRLRAGALLLSVASLPLASASLNTASIIASAAAPDCISW
                     RVSGICYWLYCSAFGCKVRTSVKVTHFIPEVVVSTYTAPGGNPWKEMSLVSRTAGGPE
                     NAITDGLSGLSAGGGNPADMKAQGERRSAIRFKYADALGHPATQIIGRMVPGFSCDSP
                     VTPAMPYFLSTLDSIVWRTGLPESLYPEALVPGKREIGSQADKNMWGSVYPRSGFVMQ
                     TDDDRAAAVVAQRVADIITRTGEPHVYREVKGVKRDGYWPPEAVEENTGTRNHKWQRL
                     TPSVSRSCAVFPDGEHQAAENGNAAFALWQPYSCCKKRGQRFLGSTNF"
     gene            complement(10309..10704)
                     /locus_tag="ACIYSB_003217"
     CDS             complement(10309..10704)
                     /locus_tag="ACIYSB_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HGC5430204.1"
                     /translation="MYRIFCSLFFLPLAASAGTTIYTDSQHLPVNPPDGVRVVLLDAP
                     EQLQTRFFGSLPADADKAEALVRERMQSPEWQMMQAELAAHYRDVAYAWSLGVKKYPA
                     VVFDDSEVVYGTTDVVVAGKLRAEGGVQP"
     gene            complement(11078..11464)
                     /locus_tag="ACIYSB_003218"
     CDS             complement(11078..11464)
                     /locus_tag="ACIYSB_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGC5430205.1"
                     /translation="MMCLKYPKPEVMTEVMPGGSVFFLPPQGKPGVADLAQPHLQRLR
                     SQLERRLGTLHRVVCQPQRVGQSSSVAVTAEGACGEVHLLLTVGGHESWPSEEEYRHP
                     RWYIQVVDAADLFYLLLWLCDGGQEA"
     gene            complement(11461..14166)
                     /locus_tag="ACIYSB_003219"
     CDS             complement(11461..14166)
                     /locus_tag="ACIYSB_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HGC5430206.1"
                     /translation="MTRDAEAAVYHAGPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     SVTPAATEGRTEERLAQIRDMVEDALQDSFDEYDTHPWVVQFYCQDENDVDAYLDYLK
                     GYVKPHAQGTAFTEAWLGEMARHLRGIARPEGLFTDTLVTGQPWRGQQRRTRMVVYRR
                     IGKNNNDPMSPVAMLNQVCDRVVSALGGAGIRCTRMNGQQVHSWLLRLFNPAPDWIAR
                     EELYRMASRVDPRERGDDVMPVMTDFAESLWFNPPYSDPENGVWWLDAMPHCAVVVEK
                     LRTPPEPGTITGEMPRGEKTVNALMDTFPEGTVMCMTIVVQPQDTLEDRFTRLSKNAV
                     GENTESIRARQDVKVVKELLGNRHKLYRAGLTFLLRAEDMDSLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHMAGLLPVTGRDTGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCASLSQVMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVRPGTGVTLPPFADAHKLVEEGQDLQCVDEH
                     SLPDIEDEAGDEGEDKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HRTYREGRQMLPEDLQSALWEISRDSSRNDVRNAKAAEMAESLGMFTQKGSFEAELFN
                     REGELWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDIVFAVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLMSASKLSGCYTEGVVLAKQIEALFRAVPPSLYLALG
                     MTEKEEKAERRMLMKEHQCSELEAAFHVARKLDRLRGLSAMEADV"
     gene            complement(14310..14714)
                     /locus_tag="ACIYSB_003220"
     CDS             complement(14310..14714)
                     /locus_tag="ACIYSB_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HGC5430207.1"
                     /translation="MKRLLFVMSVALVLSGCATSKEELLPAGEQTMLELWQGKTAGGG
                     QRHALAARETLRRPLTVTEQQGMNAEAYSYSRTQESEISQQFPRLPNPDMVMYVFPHL
                     AGGTSPVPGYSTVFPFYSRTQYALPGERTEAL"
     gene            complement(14732..16210)
                     /locus_tag="ACIYSB_003221"
     CDS             complement(14732..16210)
                     /locus_tag="ACIYSB_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HGC5430208.1"
                     /translation="MQIKSNTLVKFVVPAVVLAGLVITMKSCSTPESGSESTAQSGAL
                     AGLSSEELRALGVEGDTPEDTLRTLVARLNQVRDNQKTLESQNAQLLKDNEQLRRRGT
                     DVSGQVSEAVAGMQKTYDQKQRELQEQQRSLMSQIDVLRGQLSDQLTGSHTKLSDIPD
                     GLGLDSGSGAGSLISQGADGLMWVSPADQSQDDPLSSGGKSGPRFPTSFLEDNPLTRQ
                     KAAYEQQVKGYSNEQQAATTQAVYTLPENSTLTGSRAMTALLGRVPVNGTVTDPYPFK
                     ILVGRDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVRTLPQA
                     DVNGTTNSNQQAKNGNNAGGIGWISDEDGIPCISGTRKSNASTYLPTIFGLAAAGAAG
                     DALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETADWVRQRYGQTFDAIYV
                     PPGKTLAVHITRQLAIDYEERGRRVRYDFTLPGEAGDRGGLD"
     gene            complement(16200..17120)
                     /locus_tag="ACIYSB_003222"
     CDS             complement(16200..17120)
                     /locus_tag="ACIYSB_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HGC5430209.1"
                     /translation="MRTCVLRNLLRPAAVMLSLSVMSLTAQADELMKWERIPLDVPLM
                     VGQERVIFADKNVRVGFPPALNGRLRVQSTGGAVYLKADSAFPKTRVQLQDVESGEMI
                     LLDVTAAEKGPTEPVRIVYDGEVNTVSRAGGSAPESAKAGSSDGRPGKVGADDGTQAR
                     RTAPRYDEPLPVLLTRYAAQSLYAPLRALEPVAGIRPVVPPLPRQITTLYPSEPLTVS
                     PVAAWSAGGRTVVALKLTSTVSRRIVLDPRALQGRFVAATFQHRWLGAAGTPEDTTML
                     YVVTAGRPEKAFVAEPVAPRRTGGEVKHAD"
     gene            complement(17117..17764)
                     /locus_tag="ACIYSB_003223"
     CDS             complement(17117..17764)
                     /locus_tag="ACIYSB_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HGC5430210.1"
                     /translation="MSRFRHALQDRDQHILTLRVFSGVLAGLLLMVTLGWMSAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAYYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILETDARRRKDAGELLDRVRVVYEIPEHGYGGRYNRNPVTVEGRDNWQVRLDLV
                     VDEYFHAEPVKRALVRYPMKVVRMEGDPELNPFALALDCYSSTPQRLEAVKGGGE"
     gene            complement(17761..18126)
                     /locus_tag="ACIYSB_003224"
     CDS             complement(17761..18126)
                     /locus_tag="ACIYSB_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HGC5430211.1"
                     /translation="METIRFLPDRLNAEPTVFRGFTTPELGLAALSGVFLGLLWTLPL
                     IPLFGWVIIPTGALLTPLLLVWTGGVWITRLKRGKPDNWLWQRIEDKKAALGVGRPAF
                     IREAQGWSLRRSRVRGGKG"
     gene            complement(18146..18466)
                     /locus_tag="ACIYSB_003225"
     CDS             complement(18146..18466)
                     /locus_tag="ACIYSB_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HGC5430212.1"
                     /translation="MGLIAFLLGSLTPALADLPRIEPPSSGGGGGFFNQLKGYIQDGI
                     VLFGLVVAAAAFVKVAEAAVTTFSEVRDGKTGWGQFGAIIVVGVVLLVAVIWLLGKSA
                     NIIL"
     gene            complement(18539..18781)
                     /locus_tag="ACIYSB_003226"
     CDS             complement(18539..18781)
                     /locus_tag="ACIYSB_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HGC5430213.1"
                     /translation="MALTSAQITAFKVASGDVDLNVVHLTSVGMLVAVLFLWAAWGLS
                     DAWSGWRNGDVRESSFIWFTVRTALLLVSSIWMFAG"
     gene            complement(18781..19122)
                     /locus_tag="ACIYSB_003227"
     CDS             complement(18781..19122)
                     /locus_tag="ACIYSB_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HGC5430214.1"
                     /translation="MFSLPAFPRRAAALAGTTGMMLLAIVAVPAQAAEKDELATAMRQ
                     LDQVRAALDRARTASLQATPHDGRYFFDYPRAQADIQTMQAGIDRYLTPSRAQPRNGS
                     AVTGEYRRERP"
     gene            19366..19557
                     /locus_tag="ACIYSB_003228"
     CDS             19366..19557
                     /locus_tag="ACIYSB_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430215.1"
                     /translation="MEFLLTALPYRALFSLTWFFVVLYIVREEPQCPVWLYDVIRGIN
                     LLIIVITIVVIPLLPVLLR"
     gene            complement(19641..20402)
                     /locus_tag="ACIYSB_003229"
     CDS             complement(19641..20402)
                     /locus_tag="ACIYSB_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HGC5430216.1"
                     /translation="MAEARRDAERSPQSRAPRQHGLLYNLVWGWPWALVGLILSSLLL
                     SLLIEYAGMTFFWSEEGAAHSRAVMNTELGWLSTEFTRSLLLSAPAETVPAWISAAYQ
                     WLFVDSGMQTWLHAQQAGTAAGRSVSEFSSELSQWLAYHLHDYLLATVYVTVVTMVRV
                     VILVLSVPLFVMVVLVAVVEGLGRRDLRRYGAAYESSFVYHHAKKSVRPAIYVPCIIY
                     LSWPGAVYPNLLIWPAAMLLGLAVTVTVASFKKYL"
     gene            complement(20383..22500)
                     /gene="traD"
                     /locus_tag="ACIYSB_003230"
     CDS             complement(20383..22500)
                     /gene="traD"
                     /locus_tag="ACIYSB_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HGC5430217.1"
                     /translation="MSNRYVIEALLRPAVEFNTAVVAATAACICVTAPWAVALAPTVS
                     YATAGGFALLAVVRFRQGMNIIRYRRNLRRLPRYVMSTRQIPLSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGGLYRLARWLEMKTEHRLPALGKLLRHESSLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGEVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIISRALVALGERPDYLLIVRYVNNIADLYIRYAGKVI
                     RERMPELEQAIANNQNVISEEDVPRTMQNQPDAVRIWSIEMALSSEEGKKIYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGRIAELLAPDYLDMNDARPIFDWEQVIRKK
                     AVVYIGLDALSDSVVASAVGNSMFADLVSVAGHIYKHGMHAGLPGKGEGKNVVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARIGNAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLPQVEIYTKTLVSGHQDTADVSAGQDFTSSTQDRVGTVKVPLLEP
                     SDIVTLPKGQAFALLEGGQLWKIRMPLPAGDTDDVLMPENIARVAEAMRQNYHTGEGW
                     WNEPPSAQAAGTDEGGGYGGSAS"
     gene            complement(22500..23249)
                     /locus_tag="ACIYSB_003231"
     CDS             complement(22500..23249)
                     /locus_tag="ACIYSB_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430218.1"
                     /translation="MPQWANDPLLAWDATAFAAMSDFITEHYWTGQGSINVFRIVGTD
                     HPQYAGMTWLELMERGKRMDINIPLLEKNPGYYTQAEQQHAGMSFVSTDGVDWYVSAD
                     GNHRSCLARFLFHLQGEERTQLHNVAQSIYHTDRAFWSACREIHNLTDALSRHGVYIR
                     LQTRRQCVAREDLACWKVDRFSTEAQLTVDDVRAGGHDMPAVYKTLLLNAADAWREVM
                     VLQRRLEALSASPGNDLPRTWWRRLLRKGDM"
     gene            complement(23282..23794)
                     /locus_tag="ACIYSB_003232"
     CDS             complement(23282..23794)
                     /locus_tag="ACIYSB_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430219.1"
                     /translation="MMTVKRMFMFLFRITIVWPSVLTGCVMMLYFAEVAGGGVAGLEQ
                     RYGVESARWRDAPEGMVMTAQCPSRPFMAPVSAGEWRALAASDDECSQVPRSFREAAE
                     GDVQSLRRVAVTLLLASVVLEAFSACILRRVTASVRVSPHCGYGCGRGAVAHWPESSV
                     HTAPEKGERV"
     gene            complement(23791..24381)
                     /locus_tag="ACIYSB_003233"
     CDS             complement(23791..24381)
                     /locus_tag="ACIYSB_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGC5430220.1"
                     /translation="MQRQLTDLLQEGAWHGILLCVLVVLFLFFPRLWGRCRHRERASE
                     RRHRRYRAAAERVLTKLPEGGAGVKYLRKINPYVFEELLLLAFERRGMAVIRNTRYSG
                     DGGLDGQVLIDGERWLIQAKRYGRTIRPSHIDDFGDLLRREGCRGFFIHTGRTGPASR
                     ARLALWPDIELVSGQRLLKLLNGALLPDELTRGARR"
     gene            complement(24381..24908)
                     /locus_tag="ACIYSB_003234"
     CDS             complement(24381..24908)
                     /locus_tag="ACIYSB_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4695 family integrating conjugative element
                     protein"
                     /protein_id="HGC5430221.1"
                     /translation="MKRFKRYVMTTATGLLLCAAQAGAELRVIADLGGSDAGRFYEAI
                     NRQADSGTSSPVTALLPQGESAMLPVSTPEMSPGEVTPRPLQLPGLGALFLVGDDALS
                     RQWLQANAAALLERKAAGMVVSVTNESGLQALRALAPGVMMVPASGSALARRLQLDHW
                     PVLITDSGISSVVMP"
     gene            complement(24905..25561)
                     /locus_tag="ACIYSB_003235"
     CDS             complement(24905..25561)
                     /locus_tag="ACIYSB_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGC5430222.1"
                     /translation="MARGSLLMSLLLMAVSIPAGAALHDIPEGYVRVAEQHGVPAEVL
                     YAVSLTETAMVPSAIGAVMKKRNEDYRVPAVARPWPWTINVAGRGYRYASRLEAWQAL
                     QVFLKRYPARRIDVGIAQVNLGWNGHRFASTWAAFDPYTSLNTAAVILRECRRRHPDS
                     WLQAAGCYHHPAGGAPAVRYIALVRRHIDRLHRDAVSTSGVTAMNPRYIRTEPRELTP
                     "
     gene            complement(25540..26259)
                     /locus_tag="ACIYSB_003236"
     CDS             complement(25540..26259)
                     /locus_tag="ACIYSB_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HGC5430223.1"
                     /translation="MKLKSLSLALMLLSLQAMAAVQESTQQGTRTSSSSQVSTTTQEL
                     QQQAGQWGLSEEDYRRYRTLMEGPRGIQSPGLDPLTVLGIEARTDAERRVLAEKWVKA
                     EYERTEKELAFQREVNAAWKRLYPGVMPVSLGNSAGIARDTGGRLALFVRSKDCSTCD
                     ARLAAVLSSGRQVDIYLVDSQGNDEKLRQWAREHSIPADRVRSRHITLNHDGGRWLRF
                     GEGRMPVVLQQGADGWRVAAF"
     gene            complement(26264..27043)
                     /locus_tag="ACIYSB_003237"
     CDS             complement(26264..27043)
                     /locus_tag="ACIYSB_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430224.1"
                     /translation="MSEKQQEISAAAGGSGGTTPSGRAKRRPSPGLFAGLVTVVMMAI
                     AVAGLVRYTGEQDRRMTALAVQMKAYSDALALSGTELVPLRERLAETEKQLKSVAERI
                     SQLEKRPALTEEEHARTGEMAASLASLQTSLAQQGETLRTYEQRLEKVEKTSVTPVSP
                     SSAKGAGGTMKGTAAVAPQKKRMSAGVPVVSRTAPFVLTGTEQRGGRTYAAVAPRGFS
                     SLADVTLLGEGESTTGWVLERAGRGEATFRVNGRQVRVMVE"
     gene            complement(27040..27618)
                     /locus_tag="ACIYSB_003238"
     CDS             complement(27040..27618)
                     /locus_tag="ACIYSB_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HGC5430225.1"
                     /translation="MKISLLFSALSACVLLAGCQSPQKLQQRAAAVPVPSVTVSNNVR
                     PANPDVYAGAATPEVVRYDRYLLVNTAPDTVQRDPLSQVIDIRIPASLKPTVADAMRY
                     ALKQSGYTLCATGPANGVLYRQPLPAVQYQTGPVRLRSALQMMAGPAWQLEVDDVQRV
                     VCHSLREGYQLPARKLAPVPAVVKVSGAEQKK"
     gene            complement(27813..>28045)
                     /locus_tag="ACIYSB_003239"
     CDS             complement(27813..>28045)
                     /locus_tag="ACIYSB_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5430226.1"
                     /translation="SEAIFTHYPSHKGVIMTIAEQLEEKGLEKGRAEERQKALAETYA
                     SVRRMSDMGMSTEVIKQALQLSDEQIQEALNN"
BASE COUNT         6453 a         8825 c         6670 g         6097 t
ORIGIN      
        1 catggaacat tggtggtcgc cgaagcgaca agttagcgtt taaattgtaa gcacaaacta
       61 cgtggaagaa caacaaaaaa attcagtaaa gcggtatttt atacgctccc ggaaagggtt
      121 gttcaaaaag aaaaacgcag ttcccacgta tcagcgttct ttttttctta cgaataacat
      181 caaaagaaaa atctccgaca acgtcaaaat gatccgcgat agcagaacac atatcccatt
      241 cgttggacat gccctcacaa taaaaagaca caagttttcc tgaataaaga taataaccta
      301 aatcataaga ccagaaagga gagttgtcag tgatactttt aacagaagta atagtcatat
      361 aaatataatc actcataacg gtccctttta agaaaatggc tacaatataa taacagctat
      421 tcttcatgtc ctaacaactg gcaatatacc tgctcgatat aacgtcctct gccgtcaccg
      481 tgaccaagat ccatagatgt taatgcaagc gcttcatgat aactgtaacc attcataata
      541 tggtatttca ttgcacttgc ggcataactg taacgaaaac tatgtggtgt ctcatcaccg
      601 cctttcattt caccataacg acgcaatgag ttaagataat aattcattgc agacttaaga
      661 ttacctctgt cgattaatcg cccgtcatgc tcaccggcat atttaatagc ataattaaca
      721 gcctcaataa cattatttct gtcaacaatc gtggtcattc tctcacgacc gcctttcgta
      781 ccaaaaataa cacgtaattt ttcatctccc cgttctaatg cctgtttcca tgttttgagc
      841 gatttggcgg actgaatggc ctcctcattt ctcagaccaa gataacgcga caatttaacc
      901 gttgcagcga gtcctttatc tttttgttca atgcgctgaa gaatttcacg aaaacgatcg
      961 tctgtaatca gacttttcgt acctttacga ctctcacctg aaatacctaa tgctttatta
     1021 ctcagccgtg gatggctggg atcggcaagt ttattcttac caacctgacg taaaaccgcg
     1081 cgtattgctg acatttcatt ttctctcgtt cgttttgata catccgtcct gctgttcata
     1141 tataattcaa tgtgacgaac ttttaactgt gcagcagaac taatctgtac attcagacga
     1201 gcaagcgttt cagcgaatcg atgcataatc cttgtatagt cacttaactt tttaaaactc
     1261 cccttttctt ttcggacatg agcaaccaga tcctttttaa gcaatttctc acgtctggac
     1321 atctgtacat tctccttatg acctatgacc taaacaaaac agcagcacac aacacagatg
     1381 aattcttctg ttgtgccgtg aacacaacag aagaattcat ctgcatttca cctgacgaca
     1441 cggttcttcg ttacgttcct tcggtgagtg agtgcctgtg cgtcagggta acgcaaagtg
     1501 ttcaggtttt gcggggtgtc aagccggtta gtttaaccgg ccgcagaatt aactgtacct
     1561 ctgaaggtcg cccccgacat atctgcctgt cagatatatc gccgattcgg catctcacta
     1621 cattaaactc cttaaaaaac aatgatattc tgttgactga cgtttttccg gaagttttac
     1681 cggagagacg tcactacgaa acagaatgaa gttgaaaaaa aacgatcacg tcaggtcact
     1741 tcttcaaatg ttaaaaaaca tcccgtgaag agcctgttta tgcgggttcg ttgagtcgac
     1801 gttcatgcgt cactttgtca gcgtgaacgc cgacaaaagt gacgcatgca ctgcttcaaa
     1861 ctccacgtac acacgcaagc ggccaaattt caaatggcct tatgccgtgc gcacagtcat
     1921 aagaagctta tgcggaaagt cagaaaatat gttaagacgc agatccataa gcatttttta
     1981 tgaaagtctg ctgagaaaaa gtaaaagtac cggacgaaat ccggaatgcc ttcatcatca
     2041 cgtaaaccgt ggtgacatct gctcgcaaag gtgtgcagat ttacccgtac ttccggaaac
     2101 atatcccatc agcacgcaca gctcctttca ggctacggag gtgtaaaact gaccacccga
     2161 aggcgctcct cactggtcag atggagcatt ggtggtcgcc ggagcgacgt gggccacccg
     2221 aaggcgttcc tcacaggcca catggaacat tggtggtcgc cggagcgaca tgggccaccc
     2281 gaaggcgttc ctcacaggcc tcatggaaca ttggtggtcg ccgaagcgac agttaatgcg
     2341 ggaacagaat aacaaaagaa aaaagctatc gtcaacagac tgatttatgg catatgggaa
     2401 ataatgtatt acacaaataa accaacatat tgatatattt accgcatcgc gaatatcgtg
     2461 atagcatcaa caaatgaact gtcagacaaa aggtgataat atggattttg ctgttatatt
     2521 tttctggagc tttgcatgtg ttgttatatt tctgtgtctg aaatcatcaa taaacaacca
     2581 ggaaaaaatg cagtcattat tatttatatt tttgttgctg acaggtgggt atctttcatc
     2641 ccatatcttt aatacaggtt caggtaaatg gttatttata acaattgcca tcacatttct
     2701 gttaaacact gcgcttattt ttctttttat atttacaaaa gcatatttct tttcgcaaca
     2761 cgtaaataag atgcgagaaa aagcaaaaca aacaaattcc ttagacttta taaattgcct
     2821 gataaaactt cataaaaaat atccggtata cgtattatac gcaccttcag aaaacacagt
     2881 ggaaatctgt tacaatattt tcaatgtaaa tcctgttatt ggaaagaaac tttatttaaa
     2941 aacactgagt aatcgtcata tccggttcac agtaaaaaac ataatcctgc tacctgcatt
     3001 aaatgatgat tttatatgca cacttgaatc tttttataat aatagtgatg aaacaaaaga
     3061 tattattgat aactacatcc gaaaaataca gggtaatcaa caattgccgt ggctaataaa
     3121 caatgcagta cccactgaca caaaagaaaa gtgagtccat gatattaaat ccggataaaa
     3181 acaacttgta catcttttgc aaaacgcttt aatcttacac catgcacagt aatgagaagg
     3241 aataaaacga tgaacagtac ccggaaagag aaatacagtg ctttctgtcg tttactcaac
     3301 gaaacactta aatatgaatt acgtcttata ctccctccgg gacatcaggc agtaattcct
     3361 ctgcttgaat ctccacgagg agaaataagc cgggatacca ttgagaaaat gcgcgacata
     3421 ctcacaccgg atgtgacaca ccgaataaaa gaatccatca atgcctggac agaagatgag
     3481 ttaagttatc tggattgtac tgttgatgtt gagtatgtga aggaacagaa acgaaaatta
     3541 tttgcaatgc tggactgcga acaataaacc acaaaaaagg ccagcataat agctggccag
     3601 gtcttacaga gcaaaacaaa ataacccgta atatcacagg ggataatacc gcctctgtat
     3661 gctaacgcca gccggcaatg acaggcaaca agcgaaactg tttttcagaa acagatatag
     3721 aatggtgata ttcatccttt acccatgtaa ccacacagcg taggtgcatt atgaaagaac
     3781 tgtgacgttc cgcttcctcg tccgctcgct cactaccgtt cggtcgccgg actgcggcga
     3841 gcggactctc cagaatcaca tcctgataaa acagcctggt tcgctccctc taccccacta
     3901 ttcttttgag acacagatcc ccctcagggt cagttctccg acattcacct tcttcatctc
     3961 agccagcgca ttcctgcagg ccctttcgct ggcgaactcc tgagaatgaa cagcaagcgt
     4021 tggttgtctg gcaacactgg atgacactga atatggtgtt gaaagccaga aaatcagtat
     4081 ccacatactc cccccttgtt taacgcaaac ataccgcatg ttcagacatc acccggacaa
     4141 tcgcagcctt acagctccca gcccgacaca gcccccttcc ctccccagac aacagctaaa
     4201 aaaaatcccc gtaaccgggg atgaaggaat atggactgtg agtttctgtt taagtgcttc
     4261 tttcttgtga aacgtaaaat aaccgacctc agacactcat gcaatacctt tccatatcaa
     4321 tcgcgcctgt gatcgatttc tgctcatgcg ccttttttgg tgtcaacaaa acgaccgaaa
     4381 cgctggttaa agcgcgccac gttaccttct gaagcaacag ttctcagttt gccagtgtag
     4441 aaaggatgcg acttagagga aacatcaatt gttacgtacg gataggtgac accatcaagc
     4501 tcaatcacac gttcagtctt gatggtcgaa ccaattttat agtactcatc gatactggta
     4561 tcgtggaaca ccacagtacg gtattcagga tggatattcg gtttcatcgg ttttctcccc
     4621 cttgagtaaa atttttgcgc acgtaaatta catctctccc cgttcaatta caagccctgt
     4681 aacttccagc acgatgaaca gaaaacaaca ccagatgttt cacttatgta aaaatactgg
     4741 acaacatggt aaaaaaatga ttaaaaaaag tgggccgcca cgggcaatct ccctctcctt
     4801 tagccataag ggcttgcccg tggcgtgctc atcaacgaac ctttccttca gccacatcct
     4861 tcactcgctt aaacagaggc tcaagagaac ttgcctgcaa atgcgcctga gaagctgaag
     4921 cataggtgat atagacccgg cttcccttac cgttcttttc cacctcaatc gtcaggtgat
     4981 catcaaagct gcctttacgc cagtttcctc ccattttgta gtaactgccc ggcatcgcat
     5041 cccagatatg tccattgtca ataatcgcac ttgcctgcca ttttccgtca gttgtgccgt
     5101 tattgcgtat cgcattcacc tcatcatttg agtaaaactt gtaatagcgt ttcagtcggc
     5161 ctgctgcggt atccacatca acagggacat caataatatt ttcgcgttta tcctgctccg
     5221 gcggcaattg cttgttaagg atcggcatac cattatccgc gtagcccgtt cccccgttga
     5281 gcgctttcgt cacagcaaat gcaccgtcac tgattttctt gttcacagca gccatctctt
     5341 ccacagagca tgcactcaac gtggaacata ctgtagccat aacaaccagt tttgatattt
     5401 tcattcagct tctcctgata caggttttga ctcagggaga attaaggcat agccggacga
     5461 ctaaaaagag agattattct tttctcattt ttaaaaatta atcacacaat gttcagcagg
     5521 gctgagactg aagttcctgg agctattgtg acatgcagag tcacatcctg gcccacatca
     5581 caagctgtac agcaggaggt gcaaaatcgg acaattatac ctggattcgc aagcattgaa
     5641 catttatcat acaacacgta tgatagtacc aggatgatta tccacttaca ggaggccgcg
     5701 atgaccaatg aaacagaaaa agcaatgtgg agttgcaaaa cctctgacac accatggact
     5761 ttcagccgcc tgctgaaaaa ggctatctgt gcggttttac cggattctgg tccacacacg
     5821 gttcatcgtg tcaataagaa agaaaacaat tatttaacat ctgttttatc tgatatatgc
     5881 gattcaaatg aaaaagattc gaatgaagcg atgttcaatc cctttcatcc tctttatcga
     5941 gatcatcatc ctattgatag tagagaatgg agtggtagcc cttacaaaac gccagaataa
     6001 aaatatggcg atattcatga caaatatcgc cataaaaaac aggtagttat tttattcttt
     6061 taacaaaact tccagcatgc tggcctgctt gttgtgtaac atgccctgct cgattgaatc
     6121 cctcagaaat agcctgtccg atattaactc cagcccagga taatgctccc agccacaccg
     6181 ccggtaatac gagaaacatc gtgcccatca caaaattcat gatgatatca tccgacgtgt
     6241 tctgcagccc catcatgttc actcgggaat gcgtgtccga actgtacaag gccgtcagca
     6301 tccagctgtc aagccaccgc gccagctccc accagaacgt caggaagttc agcgcaaaca
     6361 ccacaaacgt cagtgtgatc agcgtgctga attcatacgc cgcaaacgcc agaatcaacg
     6421 gaagcatgat atacaccgcc atcagcagaa tactctgcac catcggcagc gcctgccgca
     6481 tcgcatcaaa cgccggaaac gctgccagac tcgcaacggc agaccccgcc gtgcctgctg
     6541 cacgcgtgac cgcattcgcc gccgtcatat ctgcattacc gccatagccg cggtacacct
     6601 caccaccctg tgacagggtc aggttcgccg gactcaccag acgtttgatg atttcctcct
     6661 ggtagttcgc ctgcgcgttc cccagcattt tcattgccgc cgaaatacgc aaccacagtc
     6721 cttcatcgac ttcctctgtc acccgcttct tcagcccgac actactgtca ctccaccatt
     6781 cacggcatgt gggataacca ccccgtcccg tatccggacg gccgctgtca cgggcgctat
     6841 tccacgggaa tgccgcacgt ggcattttcg actgcagtgt tccgtagtca ccctgcatga
     6901 acgtactgct gccaatccag ctgatatccg aaagcagctt cctgtccttc gtcatcccct
     6961 ggtcacgctg cttccactga tacagcgcca gcgagtaaca gtcgttggtg aaatcctgca
     7021 gctcgtcggc cagcgcccgg ttcgcaatga atgtccgctg cacctcaaaa cgcagttgcc
     7081 gtatatccgg ccggcaggga atggaagcca cagcagcctg cgtcacacct ttggagagtt
     7141 tatgcaccac cacccaccac accggggcgg ccgctgtcct gtcacccagg ctgctgatca
     7201 ccccgctgta cccggtatca ccgggtgctt tcggtgtcca cacaccgcag gtctgggcac
     7261 ggctgcgatc aaatttcagc gtgtccagcg tcacctgtac cagcggcaca caacaggcca
     7321 ccatcacaaa aaatgcacca tacagggcat gctcaatgcg gggcagtgag agcatccctt
     7381 tatttccctc atccgccccc tcttcccgca ctttcagcca gatcccgata accctgaagg
     7441 ccagtggcag ggcaaacagc cccgtccccg tcagcagatc ccacaggcca ttattcacca
     7501 gccagcccag cagggtcaga aagtgttcca gatagttatt cgtggtcaca gttcaacccc
     7561 cggtccgaaa aaaatgctgt actcgcaaag taacgcaaac agggcactga ccagacaaat
     7621 ccgcgcaaag ggacggcggt attccggtct gaaccccggc gcacgccaga ttttccggca
     7681 tccccatgcc agcgcaccgt agaggcacac ccgccacccc agccatccga aacgtgtctc
     7741 gtggagccag agccgtaatg cttccacttc ctgcgtatgc cgcatgcccg tatcagccac
     7801 caggagggcc agaatcatca tggcaataag gcccgacagc cacatgccgg ccaccttaac
     7861 gcccttcagg accagccccg cttttgtccc ctgagtcatc gccatacgct tactccccgt
     7921 ccggagtttc cagtgcccgg acacgactgt cagtattgtc tgcaggctgg atcatcgggt
     7981 tgctgcccac gcgctcattg tcgcgttcaa tgatggtcag cacgctgtta cgtgccagat
     8041 cgcgtttcag ctccatctcg ttcttcagcg ccatgatttc cctgtccagt gaagcgatac
     8101 gccggtcgcc ttcctgaatc gcggcatctt ctgcggccgc atacggctca gacatcccgg
     8161 tgagcagcat tctgcgcatc accagagccg tttccacggt atgcgccatc gccagttctc
     8221 ccgccagccg cgatgtcagg gacatattgt ccgggtcacg acgcagggca ttgatcaccc
     8281 ctgcagagac agccagactg ccggttttca gttttgccag attctccgct gtgggtttct
     8341 cctgaccatt caccagacgg accagttgtt ccgtgttcac ccgggtcgcc tcttccagca
     8401 ccggcgcaaa ccccgttcct gcaacggttc cctgctgctg ttcattatcc ccgctggtac
     8461 aggccttcgc atccgtacag gtgcgcatgg tacgatcacc cagcaccttc accaccgcct
     8521 tcgcggcctc ttctgcattc cggaacatct cacaggcgct gccgttacag ttgccggcac
     8581 tgacgctggc cgtactggtc acgggcagac cgttcatcat gttaaacccg gccgctgtca
     8641 gatcatgcac cggccggatg gcgggctgac ctgccccgcc acgctgctgt ccgccaatcc
     8701 atttcttacc ctccttcccg gtggcctgac gcccggcctc atcccctttc accgcatcgc
     8761 cgtcaccgct gttcaccacc ttcttgtact cttccagcat cgcagcctgc gtccatttgc
     8821 tgcgatcaga aaagtccatc atccgttttg ccatgttctg gcagttcagt tgcgccttgt
     8881 cgaaagccac attcgcctgc agcacaccgt tggtgatcat gtcgtacagg ccgggattgg
     8941 cacgctgtat caccatcgcc ggcagactgg caacggcgcc ggtcgccccc tgaatcacat
     9001 cccccatcag atttttaaaa ccttcggtca cgccgttaag ctgattaccc accgtggtct
     9061 tcaggtcaaa gttgccgcac atcagatcgc tgctccagcc caggtccata cccagcttct
     9121 gcatgttgct gcgcgtggct ggctgggaga tcaccgagcc gccacccagc gtatagaaca
     9181 gtttgtcaga tacggcgcca tccacggatt tcccgtaacc gaacgtgctg tcattcagtt
     9241 tcggcaggga aagaagatca gccatcaccg gagtactgca gcacagcatc acactcagtg
     9301 ccaccacacc tgttttcatc ttcatcatta tggttcctta aaaattcgta ctgccaagga
     9361 atcgctgccc gcgctttttg cagcaactgt agggctgcca cagtgcaaag gcggcattac
     9421 cgttctccgc tgcctggtgt tcaccatccg ggaaaacggc acaggaacgc gacactgacg
     9481 gcgtcagtcg ctgccatttg tgattgcggg tgccggtgtt ttcctccacc gcctccgggg
     9541 gccagtagcc gtcacgtttc acgcccttca cctccctgta gacatgcggc tcccccgtcc
     9601 gcgtgataat gtcggccacc cgctgggcca ccaccgccgc ggcccggtca tcatcggtct
     9661 gcatcacaaa accgcttctt ggataaacac tcccccacat gtttttatcg gcctggctgc
     9721 caatctcgcg tttgccggga accagtgctt cgggatacag ggattcaggt aatcccgttc
     9781 gccagacgat gctgtccagg gtactcagga aatagggcat ggccggcgtc accggtgaat
     9841 cacagctgaa tccgggcacc atcctgccga taatctgtgt ggccggatgc cccagcgcat
     9901 ctgcgtattt gaagcgaatg gctgaacgcc gctctccctg tgctttcata tccgccggat
     9961 ttcccccgcc ggcactcagt ccggacagac cgtcagtgat ggcattctcc gggccacctg
    10021 ccgtccggct gaccagcgac atctctttcc agggattacc acccggtgcc gtataggtgg
    10081 aaacaaccac ttccgggatg aaatgcgtca ccttcacgga ggttctgacc ttgcagccaa
    10141 acgcgctgca gtaaagccag taacaaatcc cgctgacacg ccagctgata cagtccggtg
    10201 ccgccgcgct ggcgataata ctggcagtat tcagtgacgc agacgccaga ggcagtgatg
    10261 ccacactcag taacagtgcg ccggcacgca gacgaccttt cagggacatc atggctgcac
    10321 tcctccctca gcacggagtt tcccggccac caccacatcc gtggtgccgt aaaccacctc
    10381 actgtcatca aacaccacgg cagggtattt cttcacgccc agcgaccagg catatgccac
    10441 atcgcggtaa tgagcagcca gttccgcctg catcatctgc cattcgggag actgcatacg
    10501 ttccctgacc agcgcttcag ccttatccgc atcagcaggc aacgagccaa aaaagcgggt
    10561 ctgaagctgc tccggtgcat ccagcagaac caccctgaca ccatcgggag gattaacggg
    10621 gagatgctga gaatcggtat aaattgttgt gccagcagac gctgccagcg gcaggaaaaa
    10681 cagggaacag aaaatccggt acattgtacg cctccgtcag acaatatccg acttacagcg
    10741 tgcgcataag cagcaccaat agcagcaata aattcaaaac cggaaactga aaatttaaga
    10801 ggggtgcgtt gcgtgagaat taagcgtccg tcacgccttt ataaacataa cgcactatcc
    10861 atgataatga caaccggacg cgaagcagta taatatcgcg cttttatttc actttcagcc
    10921 ataatactgc tgtgcataaa agacattgtt ttttatgtaa ccgctgtgtt aaaggttata
    10981 agggcatgtt ccggcaggcc agaattgggc ccatactccg taaaccacct gccagtaaca
    11041 tgccaccacg acacagttgc cagtagcggc agttttttca ggcttcctgc ccgccatcac
    11101 acagccagag cagcaggtag aacagatcgg ccgcatccac cacctggata taccagcgcg
    11161 gatgccggta ttcctcttca gagggccagc tctcatgccc ccctactgtc agcagaagat
    11221 gcacctcgcc acacgcccct tctgctgtca cggccacact gctgctctgc cccacccgct
    11281 ggggctgaca caccacacga tgcagcgtac ccagcctgcg ttcaagctgt gaccgcaggc
    11341 gctgcagatg cggttgcgcc agatccgcaa cacccggttt tccctgcggc ggcagaaaaa
    11401 acacgctgcc cccgggcatg acctctgtca tcacctccgg tttcgggtat ttcagacaca
    11461 tcatacatcc gcctccatgg ccgacagacc acgcagcctg tccagcttac gcgccacatg
    11521 gaacgcagcc tccagttcgc tgcactggtg ttccttcatc agcatacgcc gctcagcctt
    11581 ctcctccttc tccgtcatgc ccagcgccag atagagactt ggcggcaccg cacggaacag
    11641 cgcctcaatc tgcttcgcca gcaccacgcc ttcggtataa cagccggaca gcttactcgc
    11701 cgacatcagc accgctttct gctcctccgt cagtgattta aagcgtgcaa tgttgttcac
    11761 ttcctccggt ggcatcgtca gacacaccca ccattccgcc atattcagca tcttctccga
    11821 cacatccgga taatccttca ggttctgtgt cgccagccac agccacgctc ccagcttacg
    11881 ccacatcttc acaaccttcg tcatgtacgg cgaaagcagc ggattgaccg tcacgatatg
    11941 cgcctcatcc accgcaaaca cgatgtcacg ccccagatac tgatcacgtt cggcaatgtt
    12001 attgatcatg ttggtcatcg acaccatggt cagcgccatc tgtgcctcat acccctcacg
    12061 ggccagatgc cccaggtcaa tcagcgtcac atccgcttcc ggccacagtt cgccctcacg
    12121 gttgaacaac tccgcctcaa aactcccctt ctgggtgaac atccccagag actccgccat
    12181 ctccgccgcc tttgcattgc gcacatcgtt acggctgctg tcacgggaga tttcccacag
    12241 ggcggactgc aaatcctccg gcagcatctg ccgcccctca cggtatgtcc ggtgtgtggc
    12301 catcagcagc gcctcgcgga tcatcgccct gtccgcacgc ttcagcgcgg cctcttcttt
    12361 cgggtccccc ccggtgatca tcatcctggc tgagatttcc atctcgccca gaatgtctcg
    12421 tttatcctcc ccttcatccc cggcttcatc ctcaatatca ggcagtgagt gctcatccac
    12481 gcactgcaga tcctgccctt cctcaaccag tttatgcgca tctgcaaacg gcggcagggt
    12541 cacaccggtt cccggtctga cgctgatttt gttcaccgac agcccgagac tctcaaaata
    12601 atcggccagc agaccaaacg agttccccgc ttcggcaatg aacaggcggg gacggtgtac
    12661 tgccatcacc tgcgacagtg aagcacacag cgtggccgat ttaccggccc ccgttggtcc
    12721 gaacagcagc agatgcgcgt tctgggtacg gtccagcttg ttcagcggat cgaaagtcag
    12781 cacatcacca ccccggttaa aaaaactgaa ccccggatgt ccggtccccg tatcccggcc
    12841 ggtcaccggc aacaatcctg ccatgtgctg tacccacatc agacgcgtgt accactgctt
    12901 tttgtccgta tccggatcaa aacacatcgg cagagcccgc agccagctat tcagcggccc
    12961 cacgtcaaac tcaggccgca ccggctgcag accagccccc agaagcaccg tggtcagctc
    13021 cacacgccgg cgtttcaggc tgtccatatc ctctgcccgc agaagaaagg tgagccctgc
    13081 ccggtacagc ttgtgccggt tccccagcag ctccttcacc accttcacgt cctgacgcgc
    13141 ccggatggat tccgtgttct cacccaccgc atttttcgac agccgggtga aacggtcctc
    13201 cagggtgtcc tggggctgca ccacgatggt catgcacatc accgttccct ccggaaaggt
    13261 gtccatcagc gcattcaccg ttttttcccc acggggcatc tctccggtga tggtccccgg
    13321 ctccggcggc gtacgcagct tttccaccac cacggcacag tgcggcatgg cgtccagcca
    13381 ccacaccccg ttctccggat cggaatacgg cgggttaaac cacaggctct cggcaaaatc
    13441 cgtcatcacc ggcatcacat catcaccacg ctcacgcgga tcaacccgtg aggccatccg
    13501 gtacagctct tcacgggcaa tccagtccgg tgccggatta aataaccgca gcagccagct
    13561 gtgcacctgc tgaccgttca tccgggtaca gcggataccg gctccaccca gtgcggacac
    13621 cacgcggtcg cagacctggt tcagcatcgc caccggcgac atcggatcgt tattgttctt
    13681 cccgatacga cggtaaacca ccatccgggt tcgtcgctgc tgtccccgcc agggctgacc
    13741 ggtgaccagg gtgtccgtaa acagtccctc aggacgggca atccccctga gatggcgggc
    13801 catttcaccc agccaggcct ccgtgaatgc cgttccctgc gcgtggggtt tcacataccc
    13861 cttcagatag tccaggtacg catccacatc attttcatcc tgacagtaga actgcaccac
    13921 ccagggatgc gtgtcgtact catcaaaact gtcctgcagg gcatcctcca ccatgtccct
    13981 gatttgcgcc aggcgctctt ccgtacgccc ttccgttgcg gccggcgtca ccgaatagac
    14041 cgctcccacc gacaccccgt cgtcaagcag cagacactgc tcctcatcca gatattccac
    14101 ccatggcaga aaatcaataa ccgacggacc ggcatgataa accgccgcct cagcatcacg
    14161 ggtcatctgt cccggacgtc gcaggggctg tgcccccgga ggacagggcg gaacaaaggc
    14221 cgatgaagat gcccccgctt ctgcttccgg tcgttccctg gtctctgctg tacctttttc
    14281 ctcacggcgt ctgaaaagcg agaatgtcat tacagagcct ccgtacgctc acctggcagc
    14341 gcatactgcg tcctgctgta aaaaggaaat accgtgctgt agcccggaac cggcgacgta
    14401 ccgccggcca gatgcggaaa cacatacatc accatgtcgg gattcggcag acgcggaaac
    14461 tgctggctga tttcgctttc ctgcgtccgg ctgtagctgt acgcctccgc gttcatcccc
    14521 tgctgctccg ttaccgtaag gggacggcgc aacgtttccc gggcggccag cgcatgccgc
    14581 tgaccgccac ctgcggtttt accctgccag agctccagca tcgtctgctc tccggcaggc
    14641 agcagctctt ctttagaggt ggcacagccc gacagcacca gtgccacact catcacgaac
    14701 agtaatcgtt tcacggactc attctccctg ttcagtcaag gccgccccgg tcgcccgctt
    14761 ctcccggcag ggtgaagtca taacgcacac gacgtccacg ctcttcataa tcaatcgcca
    14821 gctgacgggt aatgtgaacg gccagcgttt ttcccggcgg cacataaatg gcatcaaagg
    14881 tctgcccgta acgctgtctt acccagtccg cggtctcatt catcccgccg gaaagggcct
    14941 tacccagcgc cgcttcaccg gcactgccgg tcagcgtcga ggtgataccg ttgacattgt
    15001 tctgcgaggt gtactgcccc tcactcaggg catccccggc cgcgccggct gcggccagcc
    15061 cgaatatcgt cggcaggtag gttgacgcat tggatttacg ggtgccgctg atacacggga
    15121 tcccatcctc atccgaaatc cagccaatcc cgcctgcgtt attcccgttt ttcgcctgct
    15181 gattgctgtt ggtggtaccg ttcacatccg cctgtggcag cgtgcgtacc gtgccgtcag
    15241 caaacacaaa ggtgatgctg ttcacctgtc cgcgcacaca ggaaagcgtc cagtcaccgc
    15301 tggccgtccc tgacaccaca gccccctcca catccggcag ttcaatgccg tttgccgtga
    15361 ggttatcgcg ccccacgagg atcttgaacg gatacggatc ggtcaccgtg ccgtttaccg
    15421 gcaccctgcc cagcagggcc gtcatggcac ggctgcccgt cagggttgag ttctccggca
    15481 gggtgtacac ggcctgcgtg gtggccgcct gctgctcatt gctgtacccc ttcacctgct
    15541 gctcataggc tgctttctgc cgggtcaggg ggttatcttc aaggaaggag gtcggaaaac
    15601 gcggaccgct ctttccgccg ctgctcagcg gatcatcctg tgactgatcg gccggactca
    15661 cccacatcag cccgtccgcc ccctgcgaga tcaacgaccc ggcaccactg cctgaatcca
    15721 gtcccagccc gtcaggaata tcgctgagct tcgtgtgact tcccgtcagc tggtcactca
    15781 gttgcccccg cagcacatca atctgcgaca tcagggagcg ctgctgctcc tgcagttcac
    15841 gctgcttctg atcgtaggtt ttctgcattc ccgccacggc ttcactgacc tggccggaca
    15901 catccgtccc ccggcgacgc agctgttcgt tgtccttcag caactgcgca ttctggctct
    15961 ccagcgtttt ctggttatcc ctgacctggt tcaggcgggc aaccagggtc cgcagggtat
    16021 cctccggcgt gtccccttcc acgcccagcg cacgcagctc ttctgacgat aatccggcca
    16081 gcgcaccgct ctgtgctgta ctctcactcc cgctctccgg tgtgctgcag cttttcatcg
    16141 tgatcaccag ccccgccagt accacagcag gtaccacaaa tttcaccagg gtattggact
    16201 taatctgcat gtttcacctc cccgcccgtc ctgcgcggtg ccacaggctc tgccacaaag
    16261 gccttctccg ggcggcctgc cgtcaccaca tacagcattg tggtgtcttc cggtgtcccg
    16321 gcagcaccca gccagcggtg ctggaaggtg gccgccacaa aacggccctg cagggctcgc
    16381 ggatcaagca caatacgccg gctcaccgta ctggtgagtt tcagggccac cacggttcgt
    16441 ccgccggcgg accatgccgc caccggggat accgtcaggg gttcagacgg gtaaagcgtg
    16501 gtgatttgcc ggggaagagg tggcaccacc ggtctgatcc ccgcaaccgg ttcaagggcg
    16561 cgcagcggag catacaggga ctgtgccgca taccgggtca gcagaaccgg caagggctcg
    16621 tcataacggg gggcggtccg gcgggcctgc gtgccgtcat ccgcaccgac tttcccggga
    16681 cgtccgtcac tgctgcccgc cttcgcgctc tccggagcgc tgccgccggc acggctcacc
    16741 gtgttcacct ccccgtcata cacaatccgg acaggctcag tgggcccctt ttctgcggcc
    16801 gtcacatcca gcaggatcat ctccccgctt tccacatcct gcagctgaac ccgcgttttc
    16861 ggaaaggcgc tgtccgcttt caggtagacg gcaccgccgg tgctctgcac ccgcagcctg
    16921 ccgttcagtg caggcggaaa gcccaccctc acgtttttat cggcaaaaat gacccgctcc
    16981 tgccccacca tcagcgggac atccagcgga atacgctccc acttcatcag ttcatctgcc
    17041 tgcgccgtca gggacatcac tgacagcgac aacatcacag cggcaggacg cagcaggttg
    17101 cgcagtacac aggttctcat tcaccacctc ccttcacggc ttccagacgc tgcggcgtgg
    17161 agctgtaaca gtccagcgcc agagcaaacg gattaagttc cggatcgccc tccatacgca
    17221 ccaccttcat cggatagcgg accagcgccc gtttgaccgg ctcagcatga aaatactcat
    17281 ccaccactaa gtcgagacga acctgccagt tgtcccggcc ttccaccgtg accggattac
    17341 ggttgtaacg tccgccgtaa ccgtgctcag ggatctcata caccacacga acacgatcca
    17401 gcagttcccc cgcgtcttta cggcgtcgtg catccgtttc aaggatttcc cggcacgacg
    17461 gtgtcagata ggccgacagg gcatgaattt ttgccggata atccacctca ccgtttttcg
    17521 gccaggcatt cagttgctgg aaaatgtaat acgcaaagga atagacagtg gaaggcggaa
    17581 cctcccacca cggacgggta ctgccggaac gtaaatcagg tggattatgg atggtcagcc
    17641 ggtcaggtgc agacatccag ccgagggtga ccatcagaag cagccccgcc agcacaccac
    17701 tgaacacacg aagcgtcaga atatgctggt cacgatcctg cagggcatga cgaaaacgac
    17761 tcatcctttt cctcccctga cgcgggaacg acgcagggac cagccctgtg cttccctgat
    17821 aaatgcaggg cgccccacac ccagcgccgc ttttttatct tcaatacgct gccagagcca
    17881 gttatccggt ttgccccgtt tcagccgggt gatccacacg ccgccggtcc agaccagaag
    17941 cagtggagtg agcagggcac cggtgggaat aatcacccag ccgaacagcg gtatcagtgg
    18001 cagtgtccag agaagaccga gaaagacacc gctcagtgcg gccagaccca gctctggcgt
    18061 ggtgaatccc cggaacaccg tcggttcggc attcagacga tcgggcagaa aacgaatggt
    18121 ttccataaac agccctcctg gtggattaca ggatgatgtt cgcggatttg ccgagcagcc
    18181 agataaccgc aaccagcagc accaccccga ccacgataat ggccccgaac tgtccccagc
    18241 ccgtcttgcc gtcacgcacc tcagaaaaag tggtaacggc agcctccgcc accttcacaa
    18301 aggccgccgc tgcaactacc agaccaaaca gcacaatgcc atcctggata tagcctttga
    18361 gttgattaaa aaaaccacca cccccaccgg atgatggcgg ctcaattctg ggcaaatcgg
    18421 ccagtgccgg ggtcagactg cccagcagaa aagcaatcag ccccattccc gccagacgca
    18481 ggtgacggaa agtaaaaacc tttttacaca tgtatgtctc cttaaagaaa acggaaaatc
    18541 agcccgcaaa catccagata ctgctgacaa gcagcagtgc ggtacggacg gtaaaccaga
    18601 taaacgaaga ttccctgacg tcaccgttac gccacccgga ccatgcatcc gacagtcccc
    18661 aggcggccca gagaaagaga acggcaacca gcattcccac cgatgtcaga tgcaccacat
    18721 tcagatccac atcaccggat gccaccttaa aggccgtgat ctgtgccgac gtcagcgcca
    18781 tcagggacgc tcccgccggt attcaccggt cacggcactg ccgtttcggg gctgggcacg
    18841 ggacggcgtg agataacggt caatacccgc ctgcatggtc tgaatgtcag cctgtgcgcg
    18901 cgggtagtcg aagaaataac gcccgtcatg aggcgtggcc tgaagggatg ccgttctggc
    18961 acggtcaagc gccgcccgta cctggtcaag ctggcgcatg gccgttgcca gctcatcctt
    19021 ttcggcggcc tgagccggca cagccacaat ggcaagaagc atcatcccgg tagtacctgc
    19081 cagggcagcg gcacgccggg gaaatgcggg aagagaaaac atacgcacct ccgttaagaa
    19141 tctgtgggtg cgtacagcat gaagaaggca ggtgtgccgg tcagcgataa actctatttg
    19201 taaaactgaa aatttttaag ggaaaacgga catggctgaa tgccggaaca tatccccgcg
    19261 ccggggcgca attttattcc atctttaatg attttttctg tttactgaaa caggtcatca
    19321 ctacagtttc ccgctaatga ccccccgtaa agaggaaaca tcagcatgga atttctcctc
    19381 accgcccttc cgtaccgggc cctgttcagt cttacctggt tttttgttgt gttatatatc
    19441 gtcagggaag agccacagtg ccctgtctgg ttatatgacg tcatcagggg catcaacctg
    19501 ctcatcatcg tcatcaccat tgttgtcatt cccttgctgc cggtgctctt acgctgacgc
    19561 gctgtgacag tcagtacgtg gcttgtctgc cgacaccccg ccacaaaaca cgttttgcac
    19621 cggggtgtcc ggcgcatgaa tcagagatat tttttgaacg atgccacagt cacggtaacc
    19681 gccagtccca gcagcatggc cgccggccat atcagcaggt tcggatatac cgcgccgggc
    19741 caggaaaggt aaataatgca ggggacataa atcgccggtc tgactgattt ttttgcatga
    19801 tgatagacaa agctggactc ataagccgcg ccgtaacgcc gcaaatcacg gcgccccagc
    19861 ccctccacaa cggccaccag aaccaccatc acaaacaacg gaacggacag gacgagaata
    19921 accaccctga ccatcgtcac caccgtcaca tacaccgtcg ccagcaggta gtcatgaaga
    19981 tgataagcca gccactgact cagctcactg ctgaattcag acacgcttct gcctgccgcc
    20041 gtccccgcct gctgtgcatg cagccatgtc tgcatgccac tgtccacaaa cagccactga
    20101 tacgcggcgg aaatccacgc cggcaccgtt tctgccggcg cggaaagcag cagactgcgg
    20161 gtgaactccg ttgacagcca gcccagctcc gtgttcatga cagcgcggct gtgcgccgcc
    20221 ccctcctcac tccagaaaaa cgtcataccg gcatactcaa tcaacagact cagcagcagg
    20281 gaggataaaa tcagccccac cagtgcccac ggccagcccc acaccagatt gtacagcaga
    20341 ccatgctgac ggggcgcccg ggactgcggg ctgcgttccg catcacgacg cgcttccgcc
    20401 atatccgcct ccttcatctg ttccggcggc ctgcgctgac ggcggctcat tccaccatcc
    20461 ttcaccggtg tgatagttct gtctcatcgc ctctgccacc cgggcaatgt tctccggcat
    20521 cagcacgtca tccgtatccc ctgccggcag cggcatccgg attttccaca actgcccccc
    20581 ttccagcagg gcaaacgcct gtcccttggg cagggtgaca atatctgacg gctccagcag
    20641 aggtaccttc accgtgccga cacgatcctg ggtgctggac gtgaaatcct gaccggcaga
    20701 cacgtccgcc gtatcctggt gaccggagac cagcgtcttg gtgtagatct ccacctgagg
    20761 cagttgtgtg gtcagcagct ctgccgtcct gttctccctg acacgcagca tgatcatgtt
    20821 gttgaagttt ccctgaacct gcgccgtttt ggctgcatta ccgatacgcg cttcaatatc
    20881 tgacgatgtc tgggtgtacg cggtgacctg cataccggca ccgcccccct tgttaatgag
    20941 cggaataaac tcatccccca tcagttcgtt aaactcatcg cagtgcaggt tcaccacatt
    21001 tttgccttcc cccttaccgg gcagaccggc atgcatgccg tgcttgtaga tatgccccgc
    21061 cacgctcacc aggtccgcaa acatcgaatt ccccacagca ctggctacca cactgtcgct
    21121 gagtgcatcc aggccgatat agaccaccgc ctttttacgg atcacctgtt cccagtcaaa
    21181 aatcggtcgc gcatcgttca tgtccagata gtccggtgcc agcagctctg cgatcctgcc
    21241 cgtggtgagt ttttccagca gcggcagcag ggacgccacg attttgtcaa aataggtacg
    21301 gtcatagcgg accgcactgc gaagcccgtc caggatcgga tcgtaaattt ttttaccctc
    21361 ctcactgctc agggccatct cgatggacca gatacgtacc gcatccggct ggttctgcat
    21421 ggtgcgggga acatcctcct cgctgatcac attctggtta ttcgcaatcg cctgctccag
    21481 ttccggcatg cgctcgcgga tcacctttcc ggcatagcgg atatagagat cggctatgtt
    21541 gttcacatac cgcacaatca gcaggtagtc cgggcgctca ccgagtgcca ccagggcgcg
    21601 ggaaatgatg ttgacgaacc gccaggcgaa ctcgcggaaa gccgccgagt tcccttcacc
    21661 ggacaactgc ccggccacac gagaagccac ttccgaaaca cgcccgaagc gtcccaccgc
    21721 attatatcgg gccgaaattt ccggccatcc cagatggaaa atatacagtt catcaccccg
    21781 cccggcacgg tgtgcttccg cccagacacg cagcagcaga tcagcatcac ctttgggatc
    21841 aaacacaatg gtcacctctc ccctgcggat atcctgagtg accagcagtt ctgccaggcg
    21901 ggttttaccg acacgggtgg tgccgtagac aatggtatgc cccacacgct cccccagagc
    21961 cagcgtcaca tcctgttcat ccggctcaat accatgcagg gcgggatttc cccccaccgg
    22021 cggcagagga cgcacaggat tcagagacga ctcatgccgg agcagtttcc ccagcgcggg
    22081 cagacggtgt tccgttttca tttccagcca gcgcgccagc cggtaaagtc cgcccggctg
    22141 cagatagtgt gccacctccg gtcgcagcgt atcctggaga cgctgggtat gcttctgggt
    22201 ccagcgaaat ccccgcccca gaaacagccg ctgacggctg agcgggatct gccgtgtact
    22261 catcacatag cggggcagcc ggcgcagatt acggcgatac cggatgatgt tcatcccctg
    22321 ccggaaacgc accacggcca gcagggcaaa tccaccggca gtggcgtaac tgacggtggg
    22381 tgccagcgcc accgcccagg gggctgtcac acagatacat gctgctgttg ctgccaccac
    22441 tgccgtattg aactccacag ccggtctcag caatgcctca atgacatagc ggttgctcat
    22501 tacatgtccc ccttccgtaa aagacgtcgc caccatgtgc gtggcaaatc gttccccggt
    22561 gatgcagaca gcgcctccag tctgcgctgt aataccatca cctcacgcca tgcgtctgcc
    22621 gcattcagca gcaacgtctt atacaccgca ggcatatcat gtccgccggc acgcacgtca
    22681 tccactgtca gctgcgcctc tgtgctgaaa cggtccactt tccagcaggc caggtcttcg
    22741 cgggcaacac actgccgtcg ggtctgcaga cggatataca caccatgccg ggacagcgcg
    22801 tcagtcaggt tgtggatttc ccggcaggca gaccagaacg cccggtctgt gtgataaata
    22861 ctctgtgcca cgttgtgaag ctgtgtgcgc tcctccccct gcaggtgaaa cagaaagcgt
    22921 gccaggcaac tgcggtgatt gccgtctgca ctgacatacc agtcaacgcc atccgtgctg
    22981 acaaaactca tcccggcatg ctgttgctct gcctgcgtgt agtatcccgg atttttttcc
    23041 agtaacggga tattgatgtc catacgttta ccgcgctcca tcagctccag ccaggtcatt
    23101 ccggcatact gtggatggtc agttcccaca atgcggaaca cattgatgct gccctgtccc
    23161 gtccagtagt gctctgtaat aaaatccgac attgccgcaa acgcggtggc atcccatgcc
    23221 agtaacgggt cattcgccca ttgcggcata tccctctgca cctgttcagt aaatgaaacg
    23281 ttcacacccg ctctcctttc tccggtgcag tgtgaacgct gctctctggc cagtgcgcca
    23341 ccgcaccacg tccgcagcca tatccacagt gtggtgatac ccggacggat gccgttaccc
    23401 ggcgcagaat gcaggctgaa aacgcttcca gaaccacgga tgccagcagc agtgtcactg
    23461 ccactctgcg cagcgactgc acatcgccct ctgcagcttc tctgaaagag cgcggaacct
    23521 ggctgcattc atcatcagat gcagccagtg cacgccactc cccggcactc acaggcgcca
    23581 taaagggacg cgacgggcac tgcgctgtca tcaccatccc ttccggcgca tcacgccagc
    23641 gggcggattc caccccgtaa cgctgctcca gtcccgccac accaccgccg gcgacctccg
    23701 cgaaatacag catcatcacg catcctgtca gcacggatgg ccagacaatc gttattctga
    23761 ataaaaacat aaacatccgt tttaccgtca tcatctccgc gctcctctgg taagttcatc
    23821 cggcagcaat gccccgttca gcagtttcag cagacgctgc ccgctgacca gctcaatatc
    23881 cggccacagc gccagccgtg cccggctggc cggtcccgta cgaccggtgt gaataaaaaa
    23941 tccacggcag ccttcacggc gcagcaggtc accaaaatca tcaatatgtg acggacggat
    24001 ggtccgtccg taacgtttgg cctgaatcag ccaccgctcg ccgtcaatca gcacctgacc
    24061 atccaggcca ccatctccgc tgtaacgtgt gttacggatg accgccatcc ccctgcgttc
    24121 aaacgccaga agcagcagtt cttcaaacac atacgggtta atttttcgca ggtatttcac
    24181 acccgcccca ccttccggca gctttgtcag cacacgttca gccgcagcgc ggtaacggcg
    24241 gtgacgacgc tcactggcgc gttcacgatg cctgcatcgc ccccacaggc gcggaaaaaa
    24301 cagaaacagc acgaccagaa cgcacagcag aatgccgtgc cacgcgcctt cctgtaacag
    24361 gtccgtcagt tgtctctgca tcacggcatc accacactgc tgataccact gtccgttatc
    24421 agcaccggcc agtggtcaag ctgcagacgg cgggccagtg ctgaaccgga agccggtacc
    24481 atcatcacac cgggagccag cgcccgcagc gcctgcagac cgctctcatt cgtcacgctc
    24541 accaccatac cggcagcctt cctttcaagc agcgcggccg cattcgcctg cagccactga
    24601 cgggagagtg catcatcccc cacgaggaac agtgccccca gccccggtaa ctgcagtggc
    24661 ctcggcgtca cctctccggg agacatttcc ggcgtggaaa caggcagcat cgcactctcc
    24721 ccctgcggca gcagcgccgt tacaggcgac gacgttccgc tgtccgcctg gcggtttatt
    24781 gcttcataaa acctgccggc atcactgcca ccaaggtcag caatcaccct gagctccgcg
    24841 ccggcctgag ccgcgcacag caacagaccc gttgcggtcg tcatgacata tcgtttaaat
    24901 cgtttcatgg ggttaactcc cggggttctg tccggatata acggggattc atggcagtca
    24961 cacctgacgt actgaccgca tcacggtgga ggcggtcaat atgccgcctc accagcgcga
    25021 tgtagcgtac tgcgggtgcg ccgcctgccg gatggtgata acagccggca gcctgcagcc
    25081 agctgtcagg atggcgacgg cgacattctc tcaggatgac cgccgccgtg ttcagactgg
    25141 tgtacgggtc aaatgccgcc catgtggacg caaaacggtg cccgttccag cccagattga
    25201 cctgggctat ccccacatca atccgtcggg ccggatatcg tttcagaaaa acctggaggg
    25261 cctgccaggc ctccagtcga ctggcatagc ggtatccccg gcccgccaca ttgatggtcc
    25321 agggccaggg acgtgccact gctggtaccc tgtaatcctc attccgtttt ttcattacag
    25381 caccaatggc ggaggggacc atggccgtct cggtcagcga gacggcatag agtacttctg
    25441 ccggaacacc atgctgttcc gccacacgca cgtatccctc cgggatatca tgcagcgccg
    25501 cccccgccgg aatgctgact gccatcagca gcagcgacat cagaaggctg ccacgcgcca
    25561 tccgtcagcc ccctgctgaa gtaccaccgg cattctcccc tccccaaacc gcagccagcg
    25621 accgccgtca tggttcagcg tgatgtggcg actgcgcaca cggtctgccg ggatactgtg
    25681 ctcccgggcc cactgcctca gcttttcatc atttccctga ctgtcgacca ggtaaatatc
    25741 cacctgacga ccggatgaaa gcacggcggc cagccgcgca tcacaggtac tgcagtcttt
    25801 cgacctgaca aacagggcaa gacgtccccc ggtgtcacgg gcgatgccgg cagaattccc
    25861 caggctgacc ggcatcaccc ccggatacaa ccgtttccag gcggcattca cttcacgctg
    25921 gaaagccagt tccttctcag tgcgttcata ctcagcttta acccattttt cggccagcac
    25981 cctgcgctct gcatccgtcc gcgcttcaat ccccagtacc gtcagcgggt ccagccccgg
    26041 agactgaata ccgcggggac cttccatcag ggtacggtaa cgccggtaat cttcctcact
    26101 gagtccccac tgcccggcct gctgctgcag ttcctgtgtg gtggtactga cctgagagga
    26161 tgaggaggtg cgcgttccct gctgcgtact ctcctgtacg gcggccatcg cctgcaggga
    26221 cagcagcatg agcgccagtg acagactttt cagcttcatt ttctcactcc accatgaccc
    26281 ggacctgacg accattcacc cggaacgtgg cctcgccacg tcctgcacgc tcaagcaccc
    26341 agcctgtcgt gctctcccct tcacccagta acgtcacatc agcaagactg ctgaatccac
    26401 ggggggccac tgccgcatac gtacggcctc cccgctgctc tgtcccggtc agaacaaacg
    26461 gcgcggtacg actgaccacc ggcacgccgg cagacattcg tttcttctgt ggcgcaaccg
    26521 ccgctgtgcc cttcatcgtc ccgcccgcgc ctttcgcaga tgacggtgat accggggtga
    26581 ctgaagtctt ctccaccttc tccagtcgct gctcatacgt gcgaagcgtt tctccctgtt
    26641 gcgccagtga ggtctgcagt gatgccaggc tggcggccat ctctcccgta cgcgcatgct
    26701 cttcctccgt cagtgcagga cgcttttcca gctgagaaat acgttccgcc acactcttca
    26761 gctgtttttc ggtctcagcc agcctctccc tcagtggtac cagttccgtt ccggacaagg
    26821 cgagcgcatc agaatacgcc ttcatctgca ccgccagcgc agtcattcgc ctgtcctgct
    26881 ctcccgtata acgaaccaga ccggcaaccg cgatcgccat catcaccacc gtcaccagcc
    26941 ctgcgaacag accgggagag ggacgccgtt tcgcccttcc tgacggtgtg gtgccacctg
    27001 aaccacctgc agcggctgat atctcctgtt gcttttcact cattttttct gctccgcacc
    27061 ggataccttc accaccgccg gtaccggcgc cagcttgcgt gccggtaact gataaccctc
    27121 acgcagactg tgacagacca cgcgctgcac atcatccacc tcaagctgcc atgccggccc
    27181 cgccatcatc tgcagcgcgc tccgcagtct gaccggcccg gtctggtact gcacggccgg
    27241 tagcggctga cggtagagca caccgttggc aggccccgtg gcgcagagtg tgtagccgga
    27301 ctgtttcagg gcataacgca tcgcatccgc caccgtcggc ttcagtgatg ccggaatgcg
    27361 gatgtcaatc acctgggaaa gcgggtcacg ctgtaccgta tccggtgccg tgttaaccag
    27421 cagatagcgg tcataacgca ccacctccgg tgtcgccgca ccggcgtaca catccggatt
    27481 tgccggacgg acattgttgc tgactgtcac cgacggtacc gggacggcag ccgccctctg
    27541 ctgcagtttt tgcggactct ggcaccctgc cagcaacaca caggcagaca gcgcggaaaa
    27601 aagaagggat attttcatca gggaattacc tctgcagtga agtgataaag tcagaggtca
    27661 atttgcagaa gaagtgaggg gaggctcaat gaacaactga tttccggttg acaaaaaaaa
    27721 cgcctgcaca atggcaggcg ctgtgttcac agggcatcac gcattctgac gcatgcatca
    27781 gtcgcgctct gccacatcat tacgatgtga ggttaattat taagcgcttc ctgaatctgt
    27841 tcatcagaaa gctgaagagc ttgcttaata acttctgttg acatccccat atcactcatg
    27901 cgacggacag atgcataggt ttctgcaagc gctttttgac gctcttctgc acggcctttc
    27961 tcaaggcctt tctcttccag ttgttccgct atcgtcataa tcaccccctt gtggctggga
    28021 tagtgtgtga atattgcttc agaaa
//
LOCUS       DBBGLQ010000056        26739 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.056, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000056 DBBGLQ010000000
VERSION     DBBGLQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.056"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..91)
                     /locus_tag="ACIYSB_003240"
     CDS             complement(<1..91)
                     /locus_tag="ACIYSB_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGC5430227.1"
                     /translation="MPTLRFPFHLANHNKDAAINILIIFISIGS"
     gene            271..1740
                     /gene="gspA"
                     /locus_tag="ACIYSB_003241"
     CDS             271..1740
                     /gene="gspA"
                     /locus_tag="ACIYSB_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HGC5430228.1"
                     /translation="MSTRREVILSWLCEKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1742..2161
                     /gene="gspB"
                     /locus_tag="ACIYSB_003242"
     CDS             1742..2161
                     /gene="gspB"
                     /locus_tag="ACIYSB_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HGC5430229.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENTVEESEEKAGLRERVKNALNELER"
     gene            2399..2710
                     /gene="rpsJ"
                     /locus_tag="ACIYSB_003243"
     CDS             2399..2710
                     /gene="rpsJ"
                     /locus_tag="ACIYSB_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGC5430230.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2743..3372
                     /gene="rplC"
                     /locus_tag="ACIYSB_003244"
     CDS             2743..3372
                     /gene="rplC"
                     /locus_tag="ACIYSB_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGC5430231.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3383..3988
                     /gene="rplD"
                     /locus_tag="ACIYSB_003245"
     CDS             3383..3988
                     /gene="rplD"
                     /locus_tag="ACIYSB_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGC5430232.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3985..4287
                     /gene="rplW"
                     /locus_tag="ACIYSB_003246"
     CDS             3985..4287
                     /gene="rplW"
                     /locus_tag="ACIYSB_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGC5430233.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4305..5126
                     /gene="rplB"
                     /locus_tag="ACIYSB_003247"
     CDS             4305..5126
                     /gene="rplB"
                     /locus_tag="ACIYSB_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGC5430234.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5143..5421
                     /gene="rpsS"
                     /locus_tag="ACIYSB_003248"
     CDS             5143..5421
                     /gene="rpsS"
                     /locus_tag="ACIYSB_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGC5430235.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5436..5768
                     /gene="rplV"
                     /locus_tag="ACIYSB_003249"
     CDS             5436..5768
                     /gene="rplV"
                     /locus_tag="ACIYSB_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGC5430236.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5786..6487
                     /gene="rpsC"
                     /locus_tag="ACIYSB_003250"
     CDS             5786..6487
                     /gene="rpsC"
                     /locus_tag="ACIYSB_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGC5430237.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6500..6910
                     /gene="rplP"
                     /locus_tag="ACIYSB_003251"
     CDS             6500..6910
                     /gene="rplP"
                     /locus_tag="ACIYSB_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGC5430238.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6910..7101
                     /gene="rpmC"
                     /locus_tag="ACIYSB_003252"
     CDS             6910..7101
                     /gene="rpmC"
                     /locus_tag="ACIYSB_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGC5430239.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7101..7355
                     /gene="rpsQ"
                     /locus_tag="ACIYSB_003253"
     CDS             7101..7355
                     /gene="rpsQ"
                     /locus_tag="ACIYSB_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGC5430240.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7520..7891
                     /gene="rplN"
                     /locus_tag="ACIYSB_003254"
     CDS             7520..7891
                     /gene="rplN"
                     /locus_tag="ACIYSB_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGC5430241.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7902..8216
                     /gene="rplX"
                     /locus_tag="ACIYSB_003255"
     CDS             7902..8216
                     /gene="rplX"
                     /locus_tag="ACIYSB_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGC5430242.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8231..8770
                     /gene="rplE"
                     /locus_tag="ACIYSB_003256"
     CDS             8231..8770
                     /gene="rplE"
                     /locus_tag="ACIYSB_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGC5430243.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8785..9090
                     /gene="rpsN"
                     /locus_tag="ACIYSB_003257"
     CDS             8785..9090
                     /gene="rpsN"
                     /locus_tag="ACIYSB_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGC5430244.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9124..9516
                     /gene="rpsH"
                     /locus_tag="ACIYSB_003258"
     CDS             9124..9516
                     /gene="rpsH"
                     /locus_tag="ACIYSB_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGC5430245.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9529..10062
                     /gene="rplF"
                     /locus_tag="ACIYSB_003259"
     CDS             9529..10062
                     /gene="rplF"
                     /locus_tag="ACIYSB_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGC5430246.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10072..10425
                     /gene="rplR"
                     /locus_tag="ACIYSB_003260"
     CDS             10072..10425
                     /gene="rplR"
                     /locus_tag="ACIYSB_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGC5430247.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10440..10943
                     /gene="rpsE"
                     /locus_tag="ACIYSB_003261"
     CDS             10440..10943
                     /gene="rpsE"
                     /locus_tag="ACIYSB_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGC5430248.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10947..11126
                     /gene="rpmD"
                     /locus_tag="ACIYSB_003262"
     CDS             10947..11126
                     /gene="rpmD"
                     /locus_tag="ACIYSB_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGC5430249.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11130..11564
                     /gene="rplO"
                     /locus_tag="ACIYSB_003263"
     CDS             11130..11564
                     /gene="rplO"
                     /locus_tag="ACIYSB_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGC5430250.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11572..12903
                     /gene="secY"
                     /locus_tag="ACIYSB_003264"
     CDS             11572..12903
                     /gene="secY"
                     /locus_tag="ACIYSB_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGC5430251.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12935..13051
                     /gene="rpmJ"
                     /locus_tag="ACIYSB_003265"
     CDS             12935..13051
                     /gene="rpmJ"
                     /locus_tag="ACIYSB_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGC5430252.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13198..13554
                     /gene="rpsM"
                     /locus_tag="ACIYSB_003266"
     CDS             13198..13554
                     /gene="rpsM"
                     /locus_tag="ACIYSB_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGC5430253.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13571..13960
                     /gene="rpsK"
                     /locus_tag="ACIYSB_003267"
     CDS             13571..13960
                     /gene="rpsK"
                     /locus_tag="ACIYSB_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGC5430254.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13994..14614
                     /gene="rpsD"
                     /locus_tag="ACIYSB_003268"
     CDS             13994..14614
                     /gene="rpsD"
                     /locus_tag="ACIYSB_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGC5430255.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14640..15629
                     /gene="rpoA"
                     /locus_tag="ACIYSB_003269"
     CDS             14640..15629
                     /gene="rpoA"
                     /locus_tag="ACIYSB_003269"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGC5430256.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15670..16053
                     /gene="rplQ"
                     /locus_tag="ACIYSB_003270"
     CDS             15670..16053
                     /gene="rplQ"
                     /locus_tag="ACIYSB_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGC5430257.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            16160..16528
                     /gene="yhdN"
                     /locus_tag="ACIYSB_003271"
     CDS             16160..16528
                     /gene="yhdN"
                     /locus_tag="ACIYSB_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGC5430258.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16539..16964
                     /gene="zntR"
                     /locus_tag="ACIYSB_003272"
     CDS             16539..16964
                     /gene="zntR"
                     /locus_tag="ACIYSB_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGC5430259.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            17020..17238
                     /gene="arfA"
                     /locus_tag="ACIYSB_003273"
     CDS             17020..17238
                     /gene="arfA"
                     /locus_tag="ACIYSB_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGC5430260.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(17235..17645)
                     /gene="mscL"
                     /locus_tag="ACIYSB_003274"
     CDS             complement(17235..17645)
                     /gene="mscL"
                     /locus_tag="ACIYSB_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGC5430261.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17775..19151)
                     /gene="trkA"
                     /locus_tag="ACIYSB_003275"
     CDS             complement(17775..19151)
                     /gene="trkA"
                     /locus_tag="ACIYSB_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGC5430262.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(19173..20462)
                     /gene="rsmB"
                     /locus_tag="ACIYSB_003276"
     CDS             complement(19173..20462)
                     /gene="rsmB"
                     /locus_tag="ACIYSB_003276"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGC5430263.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKTGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20508..21455)
                     /gene="fmt"
                     /locus_tag="ACIYSB_003277"
     CDS             complement(20508..21455)
                     /gene="fmt"
                     /locus_tag="ACIYSB_003277"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGC5430264.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVAELQADVMVVVAYGLILPKAVLKM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21470..21979)
                     /gene="def"
                     /locus_tag="ACIYSB_003278"
     CDS             complement(21470..21979)
                     /gene="def"
                     /locus_tag="ACIYSB_003278"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGC5430265.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            22109..23232
                     /gene="dprA"
                     /locus_tag="ACIYSB_003279"
                     /pseudo
     CDS             22109..23232
                     /gene="dprA"
                     /locus_tag="ACIYSB_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
     gene            23204..23677
                     /gene="smg"
                     /locus_tag="ACIYSB_003280"
     CDS             23204..23677
                     /gene="smg"
                     /locus_tag="ACIYSB_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGC5430266.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23706..24248
                     /gene="yrdD"
                     /locus_tag="ACIYSB_003281"
     CDS             23706..24248
                     /gene="yrdD"
                     /locus_tag="ACIYSB_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGC5430267.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24253..24825
                     /gene="tsaC"
                     /locus_tag="ACIYSB_003282"
     CDS             24253..24825
                     /gene="tsaC"
                     /locus_tag="ACIYSB_003282"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGC5430268.1"
                     /translation="MNNNLQRDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24830..25648
                     /gene="aroE"
                     /locus_tag="ACIYSB_003283"
     CDS             24830..25648
                     /gene="aroE"
                     /locus_tag="ACIYSB_003283"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGC5430269.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25645..25902
                     /gene="yrdB"
                     /locus_tag="ACIYSB_003284"
     CDS             25645..25902
                     /gene="yrdB"
                     /locus_tag="ACIYSB_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGC5430270.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25878..26432)
                     /gene="yrdA"
                     /locus_tag="ACIYSB_003285"
     CDS             complement(25878..26432)
                     /gene="yrdA"
                     /locus_tag="ACIYSB_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGC5430271.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6850 a         6279 c         6978 g         6632 t
ORIGIN      
        1 tcgaccctat gcttataaat ataatcaata tattgatggc agcatcttta ttatgattcg
       61 ccagatggaa ggggaaacgt agtgtgggca cgatgtatgt tctaataaaa tagattgggt
      121 gggttgtata ttgacgttag tataaatagt attaatgccg cttgcatgaa tcaagcaaaa
      181 tttattcata ttagtaatat gtatttaatt aataaataca tacattattt ttatgattaa
      241 ggataatcaa taattaaaac aggaagttct atgtctacga gaagagaagt tattctttcc
      301 tggttgtgtg agaaacgtca aacctggcgt ctatgctatt tgttgggtga ggctggaagt
      361 ggaaaaacct ggctggcgca gcaactgcaa aaagataaac atcgccgtgt gattacttta
      421 agcctcgttg tttcctggca aggtaaggcc gcatggatcg ttaccgacga taacgcggct
      481 gaacagggct gccgtgacag cgcctggacg cgagatgaga tggcggggca attactgcat
      541 gcgcttcatc gaactgatag tcgctgccct cttattatta ttgaaaatgc tcacctgaat
      601 caccgccgca tacttgatga tctccaacgc gcaatatctc ttattcctga cggtcaattt
      661 ttgctgatcg gcagacccga tcgaaaagtc gaacgtgatt ttaaaaaaca gggcattgaa
      721 cttgtctcga taggacgtct gacggagcac gaacttaagg caagcatcct tgaaggtcag
      781 aatattgacc aacccgatct ccttttaacc gccagagttc tgaaacggat agctttatta
      841 tgtcggggcg atcgcagaaa gctggcgctt gccggcgaaa caattcgctt attacagcaa
      901 gccgagcaga ccagcgtttt cacagcaaaa caatggcgaa tgatttaccg catattgggt
      961 gataaccgcc cccggaaaat gcagcttgcc gtggtgatga gtggaaccat tatcgctctt
     1021 acctgtggtt ggctactgct gtcctctttt acagctacgt tacccgttcc tgcgtggctg
     1081 atacccgtaa cgccagtagt taaacaagat atgactaagg atattgctca tgtggtgatg
     1141 cgcgatagtg aggcgctaag cgtgttatac ggcgtatggg ggtacgaggt cccagctgat
     1201 tcggcctggt gcgatcaggc agtaagagcg gggctggcat gtaaaagtgg gaatgcttct
     1261 ttacaaacat tggtagatca gaatttgccc tggatcgcat cgcttaaagt cggggacaaa
     1321 aaattgcctg ttgtggtcgt tcgcgtcggt gaggctagcg ttgatgtcct cgtaggtcag
     1381 caaacgtgga cgctgacaca taaatggttt gagtcggtat ggacgggtga ttatcttctg
     1441 ttgtggaaaa tgtcgccgga aggggagagt accattacgc gtgatagcag cgaggaagag
     1501 attctctggc tggaaacgat gttaaaccgc gcattgcata tttcgacaga accttcggcg
     1561 gagtggcgtc ccttgttggt agaaaaaata aagcaatttc aaaaaagcca ccacttgaaa
     1621 actgacggtg ttgttggctt ttcaacgtta gtacatctct ggcaggtggc aggggagagt
     1681 gcctacttat atcgggatga agcaaatatt tccccggaaa caacagtgaa ggggaaatga
     1741 catgtttgaa ttctatatcg ccgcgcgtga gcagaaagaa acaggtcacc ctggtatctt
     1801 ctcccgacaa aaacacagca ccataatata tgtaatttgt ttattactga tttgcctgtg
     1861 gtttgcagga atggtgctgg ttggtgggta tgccaggcag ctatgggtgc tttggatagt
     1921 aaaagccgaa gtcactgtag aggctgaaac gcccgctttt aaacaatcaa cacaacacta
     1981 tttcttcaaa aaacagccac tccccgtcgt cgaatccgtc gaggaggaag atgacccagg
     2041 cgtcgcggtt gagaatgccc cctcttcttc tgaagacgaa gaaaatactg tggaagaaag
     2101 cgaagagaaa gccggtttaa gagagcgtgt caaaaatgca ctgaacgaac ttgagagata
     2161 acccgaaggc tgtttacttt actagcaata cgcttgcgtt cggtggttaa gtatgtataa
     2221 tgcgcgggct tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag ccgaatttgg
     2281 ctacctaaca atgctcccaa tcggggagct acgtaagaac ggttacactc tcccatcaat
     2341 cgtaatgggt ctgaggagta atcattttcg tttataaaat aattggagct ctggtctcat
     2401 gcagaaccaa agaatccgta tccgcctgaa agcgtttgat catcgtctga tcgatcaagc
     2461 aaccgcggaa atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg gtccgatccc
     2521 gctgccgaca cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca acaaagacgc
     2581 gcgcgatcag tacgaaatcc gtactcactt gcgtctggtt gacatcgttg agccaaccga
     2641 gaaaaccgtt gatgctctga tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag
     2701 cctgggttaa tcaggtcatt gagcgattga gaggttgaaa caatgattgg tttagtcggt
     2761 aaaaaagtgg gtatgacccg tatcttcaca gaagacggcg tttctatccc agtaaccgta
     2821 atcgaagttg aagcaaaccg cgttactcag gttaaagacc tggctaacga tggctaccgt
     2881 gctattcagg tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc
     2941 cacttcgcta aagctggcgt agaagctggc cgtggtctgt gggaattccg cctggctgaa
     3001 ggcgaagagt tcactgtagg tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa
     3061 gttgacgtaa ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac
     3121 ttccgtaccc aggacgctac tcacggtaac tccttgtctc accgcgttcc gggttctatc
     3181 ggtcagaacc agactccggg caaagtgttc aaaggcaaga aaatggcagg tcagatgggt
     3241 aacgaacgtg taaccgttca gagccttgac gtagtacgcg ttgacgctga gcgcaacctg
     3301 ctgctggtta aaggtgctgt cccgggtgca accggtagcg acctgatcgt taaaccagct
     3361 gtgaaggcgt aaggagatag caatggaatt agtattgaaa gacgcgcaga gcgcgctgac
     3421 tgtttccgaa actaccttcg gtcgtgattt caacgaagcg ctggttcacc aggttgttgt
     3481 tgcttatgca gctggtgctc gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac
     3541 tggttccggt aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat
     3601 caagagcccg atctggcgtt ctggtggcgt gacctttgct gctcgtccgc aggaccacag
     3661 tcaaaaagtt aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt
     3721 acgtcaggat cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga aaactaagct
     3781 gctggcacag aaactgaaag acatggctct ggaagatgtg ctgatcatca ccggtgagct
     3841 ggacgaaaac ctgttcctgg ctgcgcgcaa cctgcacaag gttgacgtac gcgatgcaac
     3901 tggtatcgac ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt
     3961 taagcaagtt gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt
     4021 gcaccgcacg tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc
     4081 aaagttgcta aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa
     4141 gtcgaagtcg aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag
     4201 cgtatcggtc gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat
     4261 ctggacttcg ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca gttgttaaat
     4321 gtaaaccgac atctccgggt cgtcgccacg tagttaaagt ggttaaccct gagctgcaca
     4381 agggcaaacc ttttgctccg ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca
     4441 atggccgtat caccactcgt catatcggtg gtggccacaa gcaggcttac cgtattgttg
     4501 acttcaaacg caacaaagac ggtatcccgg cagttgttga acgtcttgag tacgatccga
     4561 accgttccgc gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt tacatcctgg
     4621 cccctaaagg cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac
     4681 caggtaacac cctgccgatg cgcaacatcc cggttggttc tactgttcat aacgtagaaa
     4741 tgaaaccagg taaaggcggt cagctggcac gttccgctgg tacttacgtt cagatcgttg
     4801 ctcgtgatgg tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag
     4861 cagactgccg tgcaactctg ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg
     4921 gtaaagcagg tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga
     4981 acccggtaga ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa
     5041 ctccgtgggg cgttcagacc aaaggtaaga agacccgcag caacaagcgt actgataaat
     5101 tcatcgtacg tcgccgtagc aaataatttt agaggataag ccatgccacg ttctctcaag
     5161 aaaggtcctt ttattgacct gcacttgctg aagaaggtag agaaagcggt ggaaagcgga
     5221 gacaagaagc ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa catgatcggt
     5281 ttgaccatcg ctgtccataa tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg
     5341 gttggtcaca aactgggtga attcgcaccg actcgtactt atcgcggcca cgctgctgat
     5401 aaaaaagcga agaagaaata aggtaggagg aagagatgga aactatcgct aaacatcgcc
     5461 atgctcgttc ttctgctcag aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag
     5521 tgtcgcaggc tctggatatt ttgacctaca ccaacaagaa agcggctgta ctggtcaaga
     5581 aagttctgga atctgccatt gctaacgctg aacacaacga tggcgctgac attgacgatc
     5641 tgaaagttac gaaaattttc gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg
     5701 caaaaggtcg tgcagatcgc atcctgaagc gcaccagcca catcactgtg gttgtgtccg
     5761 atcgctgaga ctctggagac tagcaatggg tcagaaagta catcctaatg gtattcgcct
     5821 gggtattgta aaaccatgga actctacctg gtttgcgaac accaaagaat tcgctgacaa
     5881 cctggacagc gattttaaag tacgtcagta cctgactaag gaactggcta aagcgtccgt
     5941 atctcgtatc gttatcgagc gtccggctaa gagcatccgt gtaaccattc acactgctcg
     6001 cccgggtatc gttatcggta aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc
     6061 ggacatcgct ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc ctgaactgga
     6121 cgcaaaactg gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg
     6181 tgctatgaag cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta ttaaagttga
     6241 agttagcggc cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg
     6301 cgtaccgctg cacactctgc gtgctgacat cgactacaac acctctgaag cgcacaccac
     6361 ttacggtgta atcggcgtta aagtgtggat cttcaaaggc gagatcctgg gtggtatggc
     6421 tgctgttgaa caaccggaaa aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg
     6481 taaataagga gcgtcgctga tgttacaacc aaagcgtaca aaattccgta aaatgcacaa
     6541 aggccgtaac cgcggtctgg cgcagggtac ggatgttagc ttcggcagct tcggtctgaa
     6601 agctgttggc cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac
     6661 ccgtgcagtt aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca aaccgatcac
     6721 tgaaaagccg ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt attgggttgc
     6781 cttgattcag ccgggtaaag tcctgtatga aatggacggt gttccggaag agctggcccg
     6841 tgaagcattc aagctggcag cagcgaaact gccgattaaa accacctttg taactaagac
     6901 ggtgatgtaa tgaaagcaaa agagctgcgt gagaagagcg ttgaagagct gaacaccgag
     6961 ctgctgaacc tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag tggccagctg
     7021 caacagtctc acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg
     7081 aacgagaagg cgggtgcgta atgaccgata aaatccgtac tctgcaaggt cgcgttgtta
     7141 gcgacaaaat ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa cacccgatct
     7201 acggtaaatt catcaagcgt acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg
     7261 gtatcggtga cgtggttgaa atccgcgaat gccgtccgct gtccaagact aaatcctgga
     7321 cgctggttcg cgttgtagag aaagcggttc tgtaatacag tacactctct caatacgaat
     7381 aaacggctca gaaatgagcc gtttattttt tctacccata tccttgaagc ggtgttataa
     7441 tgccgcgccc tcgatatggg gatttttaac gacctgattt tcgggtctca gtagtagttg
     7501 acattagcgg agcactaaaa tgatccaaga acagactatg ctgaacgtcg ccgacaactc
     7561 cggtgcacgt cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg
     7621 cgtaggcgac atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa
     7681 aggtgatgtg ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg
     7741 ttctgtcatt cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc
     7801 tatcggtacg cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa
     7861 aattatctct ctggcaccag aagtactcta aggagcgaat catggcagcg aaaatccgtc
     7921 gtgatgacga agttatcgtg ttaaccggta aagataaagg taaacgcggt aaagttaaga
     7981 atgtcctgtc ttccggcaag gtcattgttg aaggtatcaa cctggttaag aaacatcaga
     8041 agccggttcc ggccctgaac caaccgggtg gcatcgttga aaaagaagcc gctattcagg
     8101 tttccaacgt agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat
     8161 tcgaagacgg taaaaaagtc cgtttcttca agtctaacag cgaaactatc aagtaatttg
     8221 gagtagtacg atggcgaaac tgcatgatta ctacaaagac gaagtagtta aaaaactcat
     8281 gactgagttt aactacaatt ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa
     8341 catgggtgtt ggtgaagcga tcgctgacaa aaaactgctg gataacgcag cagcagacct
     8401 ggcagcaatc tccggtcaaa aaccgctgat caccaaagca cgcaaatctg ttgcaggctt
     8461 caaaatccgt cagggctatc cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg
     8521 ggagttcttt gagcgcctga tcactattgc tgtacctcgt atccgtgact tccgtggcct
     8581 gtccgctaag tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat
     8641 cttcccagaa atcgactacg ataaagtcga ccgcgttcgt ggtttggata ttaccattac
     8701 cactactgcg aaatctgacg aagaaggccg cgctctgctg gctgcctttg acttcccgtt
     8761 ccgcaagtaa ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa
     8821 cgcgtagctt tagctgataa atacttcgcg aaacgcgctg aactgaaagc gatcatctct
     8881 gatgtgaacg cttccgacga agatcgttgg aacgctgttc tcaagctgca gactctgccg
     8941 cgtgattcca gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt
     9001 ttcctgcgga agttcgggtt gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa
     9061 atcccgggtc tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga
     9121 cagatgagca tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc
     9181 gcgaacaaag ctgcggtcac catgccttcc tccaagctga aagtggcaat cgccaacgtg
     9241 ctgaaggaag aaggttttat tgaagatttt aaagttgaag gcgacaccaa gcctgaactg
     9301 gaacttactc tgaagtattt ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc
     9361 cgcccaggtc tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg
     9421 ggtatcgcag ttgtttctac ctctaaaggt gttatgactg atcgtgcagc gcgccaggct
     9481 ggtcttggtg gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt
     9541 gctaaagcac cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt
     9601 acgatcaaag gtaaaaacgg cgagctgact cgtactctca acgatgctgt tgaagttaaa
     9661 catgcagata ataccctgac cttcggtccg cgtgatggtt acgcagacgg ttgggcacag
     9721 gctggtaccg cgcgtgccct gctgaactca atggttatcg gtgttaccga aggcttcact
     9781 aagaagctgc agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac
     9841 ctgtctctgg gtttctctca tcctgttgac catcagctgc ctgcgggtat cactgctgaa
     9901 tgtccgactc agactgaaat cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt
     9961 gcagcggatc tgcgcgccta ccgtcgtcct gagccttata aaggcaaggg tgttcgttac
    10021 gccgacgaag tcgtgcgtac caaagaggct aagaagaagt aaggtaacac tatggataag
    10081 aaatctgctc gtatccgtcg tgcgacccgc gcacgccgca agctccagga gctgggcgca
    10141 actcgcctgg tggtacatcg taccccgcgt cacatttacg cacaggtaat tgcaccgaac
    10201 ggttctgaag ttctggtagc tgcttctact gtagaaaaag ctatcgctga acaactgaag
    10261 tacaccggta acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg
    10321 gaaaaaggca tcaaagatgt atcctttgac cgttccgggt tccaatatca tggtcgtgtc
    10381 caggcactgg cagatgctgc ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga
    10441 tggctcacat cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg
    10501 tatctaaaac cgttaaaggt ggtcgtattt tctccttcac agctctgact gtagttggcg
    10561 atggtaacgg tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc
    10621 agaaagcgat ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat aacggcactc
    10681 tgcaacaccc tgttaaaggt gttcacacgg gttctcgcgt attcatgcag ccggcttccg
    10741 aaggtaccgg tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc
    10801 ataacgttct ggctaaagcc tatggttcca ccaacccgat caacgtggtt cgtgcaacta
    10861 ttgatggcct ggaaaatatg aattctccag aaatggtcgc tgccaagcgt ggtaaatccg
    10921 ttgaagaaat tctggggaaa taaaccatgg caaagactat taaaattact caaacccgca
    10981 gtgcaatcgg tcgtctgccg aaacacaagg caacgctgct tggcctgggt ctgcgtcgta
    11041 ttggtcacac cgtagagcgc gaggatactc ctgctattcg cggtatgatc aacgcggttt
    11101 ccttcatggt taaagttgag gagtaagaga tgcgtttaaa tactctgtct ccggccgaag
    11161 gctccaaaaa ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg
    11221 gtggtcgtgg tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg
    11281 agggtggtca gatgcctctg taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag
    11341 cagcgattac agccgaaatt cgtctgtctg acctggctaa agtagaaggc ggtgtagtag
    11401 acctgaacac gctgaaagcg gctaacatta tcggtatcca gatcgagttc gcgaaagtga
    11461 tcctggctgg cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg
    11521 ctcgtgctgc tatcgaagct gctggcggta aaatcgagga ataagtagca gatggctaaa
    11581 caaccgggat tagattttca aagtgccaaa ggtggcttag gcgagctgaa acgcagactg
    11641 ctgtttgtta tcggtgcgct gattgtgttc cgtattggct cttttattcc gatccctggt
    11701 attgatgccg ctgtacttgc caaactgctt gagcaacagc gaggcaccat cattgagatg
    11761 tttaacatgt tctctggtgg tgctctcagc cgtgcttcta tctttgctct ggggatcatg
    11821 ccgtatattt cggcgtcgat cattatccag ctgctgacgg tggttcaccc aacgttggca
    11881 gaaattaaga aagaagggga gtctggtcgt cgtaagatca gccagtacac ccgctacggt
    11941 actctggtgc tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct
    12001 ggtatgcaag gcctggtgat taacccgggc tttgcattct acttcaccgc tgttgtaagt
    12061 ctggtcacag gaaccatgtt cctgatgtgg ttgggcgaac agattactga acgaggtatc
    12121 ggcaacggta tttcaatcat tatcttcgcc ggtattgtcg cgggactccc gccagccatt
    12181 gcccatacta tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt
    12241 gcagtattag tatttgcagt gacgttcttt gttgtatttg ttgagcgtgg tcaacgccgc
    12301 attgtggtaa actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca
    12361 catttaccgc tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc ttccagtatt
    12421 attctgttcc cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg gaactggctg
    12481 acaacaattt cgctgtattt gcagcctggg caaccgcttt atgtgttact ctatgcgtct
    12541 gcaatcatct tcttctgttt cttctacacg gcgttggttt tcaacccgcg tgaaacagca
    12601 gataacctga agaagtccgg tgcatttgta ccaggaattc gtccgggaga gcaaacggcg
    12661 aagtatatcg ataaagtaat gacccgcctg accctggttg gtgcgctgta tattaccttt
    12721 atctgcctga tcccggagtt catgcgtgat gcaatgaaag taccgttcta cttcggtggg
    12781 acctcactgc ttatcgttgt tgtcgtgatt atggacttta tggctcaagt gcaaactctg
    12841 atgatgtcca gtcagtatga gtctgcattg aagaaggcga acctgaaagg ctacggccga
    12901 taattggtcg cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa
    12961 attatgccgt aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc
    13021 cgagccgaag cataaacagc gccaaggctg attttttcgc atatttttct tgcaaagttg
    13081 ggttgagctg gctagattag ccagccaatc ttttgtatgt ctgtgcgttt ccatttgagt
    13141 atcctgaaaa cgggcttttc agcatggaac gtacatatta aatagtagga gtgcatagtg
    13201 gcccgtatag caggcattaa cattcctgat cataagcatg ccgtaatcgc attaacttcg
    13261 atttatggcg tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa
    13321 gatgttaaga tcagtgagct gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc
    13381 aaatttgtcg ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg
    13441 gatcttggtt gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt
    13501 accaagacca acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg
    13561 tgattgaata atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc
    13621 tgacggcgtg gctcatatcc atgcttcttt caacaacacc atcgtgacta tcactgatcg
    13681 tcagggtaac gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa
    13741 atccactccg tttgcagctc aggttgcagc agagcgttgc gctgacgccg tgaaagaata
    13801 cggcatcaag aatctggaag ttatggttaa aggtccgggt ccaggccgcg aatctactat
    13861 tcgtgctctg aacgccgcag gtttccgcat cactaacatt actgatgtga ctccgatccc
    13921 tcataacggt tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg
    13981 ttggagaaag aaaatggcaa gatatttggg tcctaagctc aagctgagcc gtcgtgaggg
    14041 caccgactta ttccttaagt ctggcgttcg cgcgatcgat accaagtgta aaattgaaca
    14101 agctcctggc cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg
    14161 tgaaaagcaa aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc gtaactacta
    14221 caaagaagca gcacgtctga aaggcaacac cggtgaaaac ctgttggctc tgctggaagg
    14281 tcgtctggac aacgttgtat accgtatggg cttcggtgcc actcgtgcag aagcacgtca
    14341 gctggttagc cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca
    14401 ggttagtccg aatgacgttg taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa
    14461 agccgctctg gagctggctg agcagcgtga aaagccaacc tggctggaag ttgatgctgg
    14521 caagatggaa ggtacgttta agcgtaagcc ggagcgttct gatctgtctg cggacattaa
    14581 cgaacacctg atcgtcgagc tttactccaa gtaaagctta gtaccaaaga gaggacacaa
    14641 tgcagggttc tgtgacagag tttctaaaac cgcgcctggt tgatatcgag caagtgagtt
    14701 cgacgcacgc caaggtgacc cttgagcctt tagagcgtgg ctttggccat actctgggta
    14761 acgcactgcg ccgtattctg ctctcatcga tgccgggttg cgcggtgacc gaggttgaga
    14821 ttgatggtgt actacatgag tacagcacca aagaaggcgt tcaggaagat atcctggaaa
    14881 tcctgctcaa cctgaaaggg ctggcggtga gagttcaggg caaagatgaa gttattctta
    14941 ccttgaataa atctggcatt ggccctgtga ctgcagccga tatcacccac gacggtgatg
    15001 tcgaaatcgt caagccgcag cacgtgatct gccacctgac cgatgagaac gcgtctatta
    15061 gcatgcgtat caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt
    15121 cggaagaaga tgagcgccca atcggccgtc tgctggtcga cgcatgctac agccctgtgg
    15181 agcgtattgc ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc
    15241 tggtcatcga aatggaaacc aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg
    15301 caaccattct ggctgaacaa ctggaagctt tcgttgactt acgtgatgta cgtcagcctg
    15361 aagtgaaaga agagaaacca gagttcgatc cgatcctgct gcgccctgtt gacgatctgg
    15421 aattgactgt ccgctctgct aactgcctta aagcagaagc tatccactat atcggtgatc
    15481 tggtacagcg taccgaggtt gagctcctta aaacgcctaa ccttggtaaa aaatctctta
    15541 ctgagattaa agacgtgctg gcttcccgtg gactgtctct gggcatgcgc ctggaaaact
    15601 ggccaccggc aagcatcgct gacgagtaac cggatcacag gttaaggttt tactgagaag
    15661 gataaggtca tgcgccatcg taagagtggt cgtcaactga accgcaacag cagccatcgc
    15721 caggctatgt tccgcaatat ggcaggttca ctggttcgtc atgaaatcat caagacgact
    15781 ctgcctaaag cgaaagagct gcgccgcgta gttgagccgc tgattactct tgccaagact
    15841 gatagcgttg ctaatcgtcg tctggcattc gcccgtactc gtgataacga gatcgtggca
    15901 aaactgttta acgaactggg cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt
    15961 ctgaagtgtg gcttccgtgc aggcgacaac gcgccgatgg cttacatcga gctggttgat
    16021 cgttcagaga aagcagaagc tgctgcagag taatctgaag caacgtaaaa aaacccgccc
    16081 cggcgggttt ttttataccc gtagtatccc cacttatcta caatagctgt actctttttg
    16141 ttcatcccct ggagtattta tgtggttact tgaccagtgg gcagagcgcc atatagcaga
    16201 agcgcaagcg aaaggtgagt ttgataacct ggcaggtagc ggcgaaccat tgatactgga
    16261 tgatgattct cacgtgccac cggaattacg tgcggggtat cgcttgctga agaatgccgg
    16321 ttgcttaccg ccagaacttg agcaacggag agaagcaatt cagcttctgg atattctcaa
    16381 aggtatccgt cacgatgatc cgcaatatca agaggttagc cgtcgattgt cattactgga
    16441 attgaagctg cgacaagctg gattgagtac cgatttttta cgcggcgatt atgctgacaa
    16501 gttgttggac aaaatcaacg ataactagtg gagtatgtat gtatcgcatt ggtgagctgg
    16561 caaaaatggc ggaagtaaca cccgacacga ttcgttatta cgaaaaacag cagatgatgg
    16621 agcatgaagt gcgtactgaa ggtgggtttc gcctatatac cgaaagcgat ctccagcgat
    16681 tgaaatttat ccgccatgcc agacaactag gtttcagtct ggagtcgatc cgcgagttgc
    16741 tgtcgatccg catcgatcct gaacaccata cctgtcagga gtcaaaaggc attgtgcagg
    16801 aaagattgca ggaagtcgaa gcacggatag ccgagttgca gagtatgcag cgttccttgc
    16861 aacgccttaa cgatgcctgt tgtgggactg ctcatagcag tgtttattgt tcgattcttg
    16921 aagctcttga acaaggggcg agtggcgtta agagtggttg ttgatttttt gcactggcag
    16981 gattacactc gcgccgttaa ataaccaact ggagttttta tgagtcgata tcagcatact
    17041 aaagggcaga taaaggataa tgcgatagaa gcattactac atgatccctt attccgacag
    17101 cgcgtagaga aaaataagaa ggggaaaggc agttacatgc gaaaaggaaa acatggcaat
    17161 cgggggaact gggaggccag tggcaagaaa gtaaatcact tttttaccac tggtcttctg
    17221 ctttcaggcg cttgttaaga gcggttattc tgctctttca gcaaatcacg aatttctgtc
    17281 agtaatactt cttctttagt tggtgcaggt gcggctgctg gttcttcttt tttccgattc
    17341 agtttgttga ttagcttaat cgccataaag atggcaaagg ccacaatcag aaaatcaaag
    17401 acgttttgaa tgaagacacc gtaatgcatc acaacagcag ggatatcccc ctgcgcatcg
    17461 cgtagcgtga cagcaaactg tttaaaatcg atcccgccaa ttaataagcc cagaggaggc
    17521 atgatgatat cggcaaccag tgaagagaca atcttcccga atgccgcacc gataatgaca
    17581 cccaccgcca aatccaccac gttcccgcgc atcgcaaatt cgcgaaattc tttaataatg
    17641 ctcatgttat tctccctatg aagccgacaa ccataagtct aacaaatgtt aagccatttt
    17701 cctgccagga atgaatttaa ttaatccttg ataatcaaag ggaaataata aggcgtcatt
    17761 agacgcctta ttaattacaa gaagaaaggg cttggctgga agagtctttc gacgtcggta
    17821 ataaactttt tatctgtgag gaacataatt acgtgatcgc cttgctcaat gcgcagattg
    17881 tcattggcaa tcatcacgtc gtttccacgt accaccgctc caataatcgt tcctggcggt
    17941 agcttgattt cgtcaatgac tctgccgaca acgcgtgagg tgctttcatc accgtgagca
    18001 acggcttcaa tagcttctgc tacgccgcgg cgcaatgagg aaacaccaac aatatctgct
    18061 tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt gtggtgaaat cgcaatatcg
    18121 ataacgctcc cctgaaccag atccacataa gcgcgacgct ggatcaatac catcaccttt
    18181 ttcgcaccca tacgtttggc aagcatggcg gacatgatat tggcctcgtc atcgttggtg
    18241 acggcaataa acagatcaac ttgatcgata tgttcttcgg ccagtagttc ttgatccgac
    18301 gcatcaccaa aaaagacgat cgtattctgt aacttttccg ccagttcggc agcgcgctgc
    18361 tgattacgtt cgatgagttt aacgctgtaa tctttttcca gacgacgcgc cagccctgca
    18421 ccgatattac cgccaccaac cagcatgatc cgcttatacg gtttttccag tcgctgtaat
    18481 tcactcatca ccgcgcggat atgctgtgaa gcggcaataa agaacacttc atcaccagct
    18541 tcaacaatgg tcgaaccttg cggacgaatg gggcgatcgt ggcggaaaat tgctgccaca
    18601 cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa gtgcattacc aatcagcggg
    18661 ccgccataat aggctttaac cacagccagg ctgactttac cctcagcgaa gttcaccacc
    18721 tgcaatgcgc cgggatactc aatcagtcgg taaatattat cgataaccaa ctgctctggt
    18781 gcgatcagat gatcaatcgg cacagcatct gaatgaaata gcttatcggc atcgcgcacg
    18841 tagtctggtg agcggatacg agcgatgcga ttaggggtgt tgaaaagtga gtaggctacc
    18901 tggcaggcaa ccatattggt ttcatctgaa ctggttacag caaccagcat atcggcgtcg
    18961 tcggcacctg cctcccgcaa tacgcgtgga tgagagccat gcccctgcac gacccgcagg
    19021 tcaaatttat cctgtaaggt ccgcagacgc tcaccgttgg tatcgacaac agtaatatcg
    19081 ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa cctggccggc acccagaatg
    19141 ataattttca tcagtcgcga cccgttatct catcactttt tgattagctt agcgtaaaag
    19201 aagccgtcgc cctcttcggc accaggtaga ttttgtttac ccggttgctc tggtgttcct
    19261 gtttcgcaaa gttcggcatc agcggtacgt tgcaaaaagg ctttaatctg caggctattc
    19321 tcttccggta acaccgaaca ggtggcatag accagagttc caccggtttt taaatgcggc
    19381 caaatggcgt cgagaatttc agactgcaat tgcgcgagtt ccgggatatc gcgatcgcga
    19441 cgtaaccatt taatatctgg atggcgacga atcacaccgg ttgctgaaca aggcgcatct
    19501 aataaaatgc gatcaaactg ttgctcgcca caccattgag aagggtaacg gccatcacct
    19561 tgtttcacgg tcgccttcat accaaggcgt tttaaattgt cgtaaacccg agagaggcgc
    19621 tgttcgtcga tatcaaccgc aacaacctgc gcttctggtg ccacctcaag gatatgcgtt
    19681 gttttaccgc cgggggcggc acaaagatcc aaaatgtgtt caccgttttg tggcgcaagc
    19741 caggtcatgc aaccttgtgc tgatgcatcc tgaacggtaa cccatccgtc ttcaaaacca
    19801 ggtagcgcat gaacaggtgc aggtgtttcc agacgtacag catcagggta atccgcatgc
    19861 gggaaacctt tcattcctgc ttcatccagc aatgcaagcc agctgtcgcg ggaatgatgc
    19921 gtacgattaa tacgcagcca cattggcgga cgctggttat tggcttcgac gatggattgc
    19981 cactgctctg gatacgcttt ttgcagacgc ttcagcaacc aggaaggatg cagataacgt
    20041 gcatcactgg cattaaactc ggctaataac tcttcttgct gacgctggaa ctggcgtaat
    20101 acaccgttta tcaacccttt aagttgcgga cgcttaattg cgatagcgcc ttcaaccgtt
    20161 tcagccagcg cagcatgagg tggaatgcgg gtataaagca gttgatacaa accaaccata
    20221 atcaggtaat gcacggtccg ctgtttgccg gtcatcggac gggccattaa cttattaatc
    20281 agccagtcta actgcgaaag cgtacgcagt acgccaaagc acaactcttg aagaagtgct
    20341 ttgtctttat cggaaacttt ttgctggagc ggtggcagaa tgttgcttaa tgattgccct
    20401 tgctcgacga cttgttcaac ggcctgggcc gccatgctac gtaaattacg ttgttttttc
    20461 ataagtataa aaacgcccgg caagaccggg cttagaagag tggactatca gaccagacgg
    20521 ttgcccggaa caaaccattc ccgacgagag ttcaggaggt cttgcgcgct catcgctttc
    20581 ttacccgcag gttgtaacga gagcaggttc aggatgccat caccagtcgc aacctgaatg
    20641 ccttgtttgt tggcttcaag gatcgttcct ggtgcagcgt tggttgccgt atcaatgacc
    20701 gatgctttcc agactttaac cggctgtcct tcaatttcca gccagctcat tggccatgga
    20761 ttgaaagcgc gaatgcagcg ttcaagctgt gctgccgaaa gtgaccagtc aatacgcgct
    20821 tcttctttac tcaacttctc ggcgtaagtg acaagagttt cgtcctgaac ttctggtttc
    20881 gccgtgccgt ctgccagttg tttcaacgtg gtgataagcc cttgtgggcc aagctctgcc
    20941 agcttgtcgt acagcgtacc actggtatct tctgcagtaa tcgggcagga gagcttatag
    21001 agcatatcac cggtgtctaa accgacatcc atttgcataa tggtcacacc agtttctgca
    21061 tcacccgccc atagtgagcg ttggattggt gcagcaccgc gccagcgtgg cagcagtgaa
    21121 ccatgaacgt tgatacagcc aagacgcggc atcttcagca ctgctttcgg cagaattaaa
    21181 ccataggcga cgacgaccat aacatcagcc tgcagttcgg cgaccagttg ctggttttct
    21241 tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt tttcctcagc cagaacttta
    21301 accgggctgg gcatcagttt tttaccgcgt cctgccggtc ggtctggctg ggtgaacacg
    21361 ccaacgacgt tatgaccaga agacaacagc gcgtcgagat gacgcgctgc aaagtcaggt
    21421 gtacccgcaa aaataatacg tagtgattct gacacgttag ttcttatcct taagcccggg
    21481 ctttcagacg atccagtttt tcaactttct gacgaatacg ttgttgtttc agcggtgaca
    21541 gataatccat aaacagtttg ccgaccaggt gatccatctc atgctgaata cagatggcta
    21601 acagaccgtc tgcttccagt tcaaatggtt taccgtcgcg gtcaagggcg cgaattttaa
    21661 ctttctctgc gcgcggcact aaagcacgtt gttcagggat cgacaggcaa ccttcttcaa
    21721 tgcctgtttc gccgcttttt tctaaaagct ctggattgat taacactagc cgttcgtcac
    21781 ggttttccga aacatcaata acaatgatac gttgatggat atcaacctgg gttgccgcca
    21841 ggccaatacc ttcttctgcg tacatcgtct cgaacatatc atcgacgata cgctgaattt
    21901 ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag ccgctcgtcc ggaatatgta
    21961 acacttgcaa aactgacata aatctccaga gatgtgttca ggagttagaa agattatttc
    22021 ttctattcta gacaaatccc cctctgattg acagcatcac tgaccaatcg caaagattgc
    22081 taaggctgct tatggcaggg agataaggat ggtcgataca gaaatttggc tgcgtttaat
    22141 gagtatcagc agcttgtacg gcgatgatat ggtccgtatc gctcactggg tggcaaaaca
    22201 gtcgcatatt gatgcggttg tattgcagca aacagggctt acattgcgga ggcacaacgc
    22261 tttctttcat ttccacgaaa gagtatcgaa agctcacttt gttggttgga gcaacccaac
    22321 catcatttaa ttcctgcgga cagcgaattt tatcctcctc aacttctggc gacgacagat
    22381 taccccggcg cactgtttgt tgaaggagaa ctgcacgcgc tgcattcatt tcagcttgcc
    22441 gtagtgggga gtcgggcgca ttcatggtat ggcgagcgat ggggacgatt attttgcgaa
    22501 actctggcga cgcgtggagt gacaattacg agtggactgg cgcgtggaat cgatggtgta
    22561 gcgcataaag cagccttaca ggtaaatggc gtcagcattg ctgtattggg gaatggactt
    22621 aataccattc atccccgccg tcatgcccga ctggctgcca gtctgcttga acaggggggc
    22681 gctctcgtct cggaatttcc cctcgatgtt ccaccccttg cttacaattt cccacgaaga
    22741 aatcgcatta tcagtggtct aagtaaaggt gtactggtgg tggaagcggc tttgcgtagt
    22801 ggttcgctgg tgacagcacg ttgtgcgctt gagcaggggc gagaagtttt tgccttgcca
    22861 ggtccaatag ggaatccggg aagcgaaggg cctcactggt taataaaaca aggtgcgatt
    22921 cttgtgacgg aaccggaaga aattctggaa aacttgcaat ttggattgca ctggttgcca
    22981 gacgcccctg aaaattcatt ttattcacca gatcagcaag acgtggcatt gccatttcct
    23041 gagctcctgg ctaacgtagg agatgaggta acacctgttg acgtcgtcgc tgaacgtgcc
    23101 ggccaacctg tgccagaggt agttactcaa ctactcgaac tggagttagc aggatggatc
    23161 gcagctgtac ccggcggcta tgtccgattg aggagggcat gccatgttcg acgtactaat
    23221 gtatttgttt gaaacctata ttcacacaga agctgagttg cgtgtggatc aagacaaact
    23281 tgaacaggat cttaccgacg caggttttga gcgagaagat atctacaatg ccctgctatg
    23341 gctggaaaaa cttgctgatt atcaggaagg gttggcagaa ccgatgcaac tggcctcgga
    23401 tcctctctcc atgcgtattt atacaccgga agagtgtgaa cgactggatg ccagctgccg
    23461 tgggtttctg cttttccttg agcagattca ggtgctcaac cttgaaactc gtgaaatggt
    23521 gatagagcga gtgctcgcgc tggataacgc agagttcgaa ctggatgatc tgaaatgggt
    23581 gatcctgatg gtgttgttca atattccggg ctgcgaaaat gcgtaccagc aaatggaaga
    23641 attactcttt gaagtgaatg aaggtatgct gcattaatca tttctttaat tcagcataag
    23701 ttgttatggc gaaatcagca ctgttcacgg tgcgtaataa tgagtcctgc ccaaagtgcg
    23761 gggctgaact ggttattcga tccgggaaac acggtccgtt tcttggatgc tcacagtatc
    23821 cggcgtgtga ctacgtccgt cctctgaaat cttcagcgga tggacatatc gtcaaagttc
    23881 tggaggggca ggtttgccct gcatgtggcg caaatctggt attacgccag ggacgctttg
    23941 gtatgtttat tggttgcatt aactaccctg aatgcgaaca taccgaactt atcgataaac
    24001 cggacgaaac agcaattaca tgcccccaat gtcggacggg ccatctggtc cagcgccgct
    24061 cccgttatgg caaaacattt cactcttgtg atcgctaccc ggagtgtcaa tttgccatta
    24121 acttcaaacc catagctgga gaatgccctg agtgtcatta tccgctactc atcgaaaaga
    24181 aaaccgcgca gggtgtaaaa cacttttgtg ccagtaaaca atgtggaaag ccggtttcgg
    24241 cggaataata acgtgaataa taacctgcaa agagacgcta tcgcagctgc gatagatgtt
    24301 ctcaatgaag aacgtgtcat cgcctatcca acggaagccg ttttcggtgt tgggtgcgat
    24361 cctgatagcg aaacagcagt gatgcgactg ttggagttaa aacagcgtcc ggttgataag
    24421 gggctgattt taatcgcagc aaattacgag cagcttaaac cctatattga tgacaccatg
    24481 ttgactgacg tgcagcgtga aaccattttt tcccgctggc caggtcctgt cacctttgtc
    24541 tttcccgcgc ctgcgacaac accgcgctgg ttgacgggcc gctttgattc gcttgctgta
    24601 cgagtcaccg accatccgtt ggtggttgct ttgtgccagg cttatggtaa accgctggtt
    24661 tctaccagtg ccaacttgag tggattgcca ccttgtcgaa cagtagacga agttcgcgca
    24721 caatttggcg cggcgttccc ggttgtgcct ggtgaaacgg gggggcgttt aaatccttca
    24781 gaaatccgcg atgccctgac gggtgaactg tttcgacagg ggtaacataa tggaaaccta
    24841 tgctgttttt ggtaatccga tagcccacag caaatcgcca ttcattcatc agcaatttgc
    24901 tcagcaactg aatattgaac atccctatgg gcgcgtgttg gcacccatca atgatttcat
    24961 caacacactg aacgctttct ttagtgctgg tggtaaaggt gcgaatgtga cggtgccttt
    25021 taaagaagag gcttttgcca gagcggatga gcttactgaa cgggcagcgt tggctggtgc
    25081 tgttaatacc ctcatgcggt tagaagatgg acgcctgctg ggtgacaata ccgatggtgt
    25141 aggcttgtta agcgatctgg aacgtctgtc ttttatccgc cctggtttac gtattctgct
    25201 tatcggcgct ggtggagcat ctcgcggcgt actactgcca ctcctttccc tggactgtgc
    25261 ggtgacaata actaatcgga cggtatcccg cgcggaagag ttggctaaat tgtttgcgca
    25321 cactggcagt attcaggcgt tgagtatgga cgaactggaa ggtcatgagt ttgatctcat
    25381 tattaatgca acatccagtg gcatcagtgg tgatattccg gcgatcccgt catcgctcat
    25441 tcatccaggc atttattgct atgacatgtt ctatcagaaa ggaaaaactc cttttctggc
    25501 atggtgtgag cagcgaggct caaagcgtaa tgctgatggt ttaggaatgc tggtggcaca
    25561 ggcggctcat gcctttcttc tctggcacgg tgttctgcct gacgtagaac cagttataaa
    25621 gcaattgcag gaggaattgt ccgcgtgaat caggccatcc agtttccgga cagggaagag
    25681 tgggacgaga ataaaaaatg tgtatgtttt cccgctctcg tgaatggtat gcaactgaca
    25741 tgcgcgatct ctggcgagag tctggcgtat cgctttactg gagatacgcc agaacagtgg
    25801 ttagcgagtt ttcgtcagca tcgctgggac ctggaagaag aagcggaaaa cttaattcag
    25861 gaacaaagtg aagatgatca aggctgggtc tggttaccct gatccagata ttcgtccttc
    25921 catttcacgt aattattcgc ggaatagcgt aacccagcct tctcttcatc acttaacggg
    25981 cggatctgtt tgacggggct accgagatac agatatccgc tctccagccg tttattttgt
    26041 gggaccagac tacccgcacc aatcatcaca tcatcttcta ctattgcgcc atcaagtaaa
    26101 attgagccca tcccaaccaa aactcgattg ccaatggtgc agccgtggag catcaccttg
    26161 tgaccaacag tgacatcttc gccaatggtt aatgggttgc catctgggtt gtacgaggat
    26221 ttatgagtga catgcaacat actgccatcc tggatattgg tgcgtgctcc gatctgtaca
    26281 taatgtacat ctccacgaat cacaacgagc ggccagatcc ccacatcatc agccagacga
    26341 acgtcaccaa tcacgacact gctatcgtcg atcattacgc gctgaccgat ttgtggaaaa
    26401 agatcgcggt atgggcgtaa aacatcagac atacttacct cagcaataaa tgatttacta
    26461 atgactttgg gggcattatt ggccttgtgc aagtctttta gtatgcaaaa aagcaccgtt
    26521 ttgtgtgcga ttgcagcaaa aagggtgaaa aaacaacaaa cagaaaaaaa gatcaaaaaa
    26581 atacttgtgc aaaaaattgg gatccctata atgcgcctcc gttgagacga caacgtgaaa
    26641 cacttcacag gatggtcgga acaacgaaga gaaaaaaatc ctgaaattca gggttgactc
    26701 tgaaagagga aagcgtaata tacgccacct cgcgacagt
//
LOCUS       DBBGLQ010000057        26355 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.057, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000057 DBBGLQ010000000
VERSION     DBBGLQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.057"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            23..1036
                     /gene="galE"
                     /locus_tag="ACIYSB_003286"
     CDS             23..1036
                     /gene="galE"
                     /locus_tag="ACIYSB_003286"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGC5430272.1"
                     /translation="MAILITGGAGYIGSHTAVALLKKNYEVVIIDNLVNSHYSVIEKI
                     QKITGKTIKFYEGSISNKSLLHEIFSKNRIESVLHFAGLKSVAESTRLPLHYYNTNVS
                     ATLNLVEAMLKNDVCNFIFSSSATVYGEPENIPLNESSRVGNTTNPYGTSKLMVEKIL
                     QDISSSNQKFRTTILRYFNPVGAHKSGEIGEDPNGTPNNLMPYICQVAIGKRKELFIY
                     GNDYKTKDGTGIRDFIHVMDLAEGHIAALKKCDNGSNYKIFNLGTGKGYSVLDLISAF
                     ERVTSVTIPYVITSRRPGDIAECWSDPKKAFLELGWKAMYNLDDMVRDAWNWQKKNPS
                     GYK"
     gene            1125..2531
                     /gene="gndA"
                     /locus_tag="ACIYSB_003287"
     CDS             1125..2531
                     /gene="gndA"
                     /locus_tag="ACIYSB_003287"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGC5430273.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            2779..3945
                     /gene="ugd"
                     /locus_tag="ACIYSB_003288"
     CDS             2779..3945
                     /gene="ugd"
                     /locus_tag="ACIYSB_003288"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGC5430274.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            4091..5068
                     /gene="wzzB"
                     /locus_tag="ACIYSB_003289"
     CDS             4091..5068
                     /gene="wzzB"
                     /locus_tag="ACIYSB_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGC5430275.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(5165..5776)
                     /gene="hisIE"
                     /locus_tag="ACIYSB_003290"
     CDS             complement(5165..5776)
                     /gene="hisIE"
                     /locus_tag="ACIYSB_003290"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGC5430276.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALNK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(5770..6546)
                     /gene="hisF"
                     /locus_tag="ACIYSB_003291"
     CDS             complement(5770..6546)
                     /gene="hisF"
                     /locus_tag="ACIYSB_003291"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGC5430277.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALAEPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            complement(6528..7265)
                     /gene="hisA"
                     /locus_tag="ACIYSB_003292"
     CDS             complement(6528..7265)
                     /gene="hisA"
                     /locus_tag="ACIYSB_003292"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGC5430278.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(7265..7855)
                     /gene="hisH"
                     /locus_tag="ACIYSB_003293"
     CDS             complement(7265..7855)
                     /gene="hisH"
                     /locus_tag="ACIYSB_003293"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGC5430279.1"
                     /translation="MNVVILDTGCANLNSVKSAITRHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(7855..8922)
                     /gene="hisB"
                     /locus_tag="ACIYSB_003294"
     CDS             complement(7855..8922)
                     /gene="hisB"
                     /locus_tag="ACIYSB_003294"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGC5430280.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECE
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(8922..9992)
                     /gene="hisC"
                     /locus_tag="ACIYSB_003295"
     CDS             complement(8922..9992)
                     /gene="hisC"
                     /locus_tag="ACIYSB_003295"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGC5430281.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDDVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEELINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQHSLSGCL
                     RITVGTHEESQRVIDALRAEQV"
     gene            complement(9989..11293)
                     /gene="hisD"
                     /locus_tag="ACIYSB_003296"
     CDS             complement(9989..11293)
                     /gene="hisD"
                     /locus_tag="ACIYSB_003296"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGC5430282.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASGRLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            complement(11299..12198)
                     /gene="hisG"
                     /locus_tag="ACIYSB_003297"
     CDS             complement(11299..12198)
                     /gene="hisG"
                     /locus_tag="ACIYSB_003297"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGC5430283.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     misc_feature    complement(12245..12368)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(12344..12394)
                     /gene="hisL"
                     /locus_tag="ACIYSB_003298"
     CDS             complement(12344..12394)
                     /gene="hisL"
                     /locus_tag="ACIYSB_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGC5430284.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            12678..12929
                     /gene="yefM"
                     /locus_tag="ACIYSB_003299"
     CDS             12678..12929
                     /gene="yefM"
                     /locus_tag="ACIYSB_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HGC5430285.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            12926..13180
                     /gene="yoeB"
                     /locus_tag="ACIYSB_003300"
     CDS             12926..13180
                     /gene="yoeB"
                     /locus_tag="ACIYSB_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HGC5430286.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            13263..14087
                     /gene="yeeZ"
                     /locus_tag="ACIYSB_003301"
     CDS             13263..14087
                     /gene="yeeZ"
                     /locus_tag="ACIYSB_003301"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGC5430287.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDTDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            14133..15062
                     /gene="yeeY"
                     /locus_tag="ACIYSB_003302"
     CDS             14133..15062
                     /gene="yeeY"
                     /locus_tag="ACIYSB_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGC5430288.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            15277..15339
                     /gene="yoeI"
                     /locus_tag="ACIYSB_003303"
     CDS             15277..15339
                     /gene="yoeI"
                     /locus_tag="ACIYSB_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGC5430289.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            15329..16687
                     /gene="plaP"
                     /locus_tag="ACIYSB_003304"
     CDS             15329..16687
                     /gene="plaP"
                     /locus_tag="ACIYSB_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGC5430290.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            16866..17924
                     /gene="tsuA"
                     /locus_tag="ACIYSB_003305"
     CDS             16866..17924
                     /gene="tsuA"
                     /locus_tag="ACIYSB_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGC5430291.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            17938..18165
                     /gene="tsuB"
                     /locus_tag="ACIYSB_003306"
     CDS             17938..18165
                     /gene="tsuB"
                     /locus_tag="ACIYSB_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGC5430292.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(18208..19635)
                     /gene="sbcB"
                     /locus_tag="ACIYSB_003307"
     CDS             complement(18208..19635)
                     /gene="sbcB"
                     /locus_tag="ACIYSB_003307"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGC5430293.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYSKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            19844..21010
                     /gene="dacD"
                     /locus_tag="ACIYSB_003308"
     CDS             19844..21010
                     /gene="dacD"
                     /locus_tag="ACIYSB_003308"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGC5430294.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTSQILTAVNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            21129..21602
                     /gene="sbmC"
                     /locus_tag="ACIYSB_003309"
     CDS             21129..21602
                     /gene="sbmC"
                     /locus_tag="ACIYSB_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGC5430295.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            21800..22858
                     /gene="yeeA"
                     /locus_tag="ACIYSB_003310"
     CDS             21800..22858
                     /gene="yeeA"
                     /locus_tag="ACIYSB_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGC5430296.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEINTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            23030..23359
                     /gene="tmaR"
                     /locus_tag="ACIYSB_003311"
     CDS             23030..23359
                     /gene="tmaR"
                     /locus_tag="ACIYSB_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGC5430297.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23460..23642)
                     /locus_tag="ACIYSB_003312"
     CDS             complement(23460..23642)
                     /locus_tag="ACIYSB_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGC5430298.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            23696..23842
                     /locus_tag="ACIYSB_003313"
                     /pseudo
     CDS             23696..23842
                     /locus_tag="ACIYSB_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(24131..24211)
                     /locus_tag="ACIYSB_003314"
                     /pseudo
     CDS             complement(24131..24211)
                     /locus_tag="ACIYSB_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24257..24451)
                     /gene="yeeW"
                     /locus_tag="ACIYSB_003315"
     CDS             complement(24257..24451)
                     /gene="yeeW"
                     /locus_tag="ACIYSB_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGC5430299.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            complement(24448..24821)
                     /gene="cbtA"
                     /locus_tag="ACIYSB_003316"
                     /pseudo
     CDS             complement(24448..24821)
                     /gene="cbtA"
                     /locus_tag="ACIYSB_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            complement(24910..25278)
                     /gene="cbeA"
                     /locus_tag="ACIYSB_003317"
     CDS             complement(24910..25278)
                     /gene="cbeA"
                     /locus_tag="ACIYSB_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGC5430300.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            complement(25352..25573)
                     /gene="yeeT"
                     /locus_tag="ACIYSB_003318"
     CDS             complement(25352..25573)
                     /gene="yeeT"
                     /locus_tag="ACIYSB_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGC5430301.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(25636..26112)
                     /gene="yeeS"
                     /locus_tag="ACIYSB_003319"
     CDS             complement(25636..26112)
                     /gene="yeeS"
                     /locus_tag="ACIYSB_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGC5430302.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(26128..>26355)
                     /locus_tag="ACIYSB_003320"
     CDS             complement(26128..>26355)
                     /locus_tag="ACIYSB_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGC5430303.1"
                     /translation="PEPDDDDDETWVLFNAMNGNRAEMSPEAAGIAACLMTYSHHACR
                     TENYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         6386 a         6363 c         6776 g         6830 t
ORIGIN      
        1 tcttaaattc gagagagtgc ttatggccat attgataaca ggtggcgcag ggtatattgg
       61 gtcgcatact gctgttgcat tactgaaaaa aaattatgaa gtggtaatta ttgataattt
      121 ggtaaattct cattattctg ttattgaaaa aattcaaaag attactggaa aaacaataaa
      181 gttttatgaa ggaagtatta gtaataaaag tttattacac gagattttct caaaaaacag
      241 aatcgagtct gttttacatt ttgctgggtt gaaatccgtt gcagaatcta ctcgacttcc
      301 tctacattat tataatacta atgtttcagc aactttaaac ttagtagagg caatgctaaa
      361 aaatgatgtt tgtaatttta ttttcagctc ttctgcaact gtatatggag agccagagaa
      421 tataccgcta aacgaatctt cgcgagtcgg taatacaaca aatccttatg gaacatcgaa
      481 gttaatggtt gaaaaaatac tacaagatat ctcaagctct aaccaaaaat ttcgtacgac
      541 tatattaaga tattttaatc cagtgggagc tcacaaatcc ggtgagattg gggaagatcc
      601 taatggtaca ccaaataatt tgatgcctta catatgtcaa gtcgccattg gtaagcgaaa
      661 ggaattattt atttatggta atgattataa aacaaaagat gggacaggga ttcgtgattt
      721 catacatgta atggatcttg cagaggggca tattgctgct ttgaaaaaat gtgataatgg
      781 aagcaactat aaaattttta atttaggtac aggaaaagga tattccgtac tcgatcttat
      841 ttcagctttt gagcgtgtta catctgttac cataccatat gtcataacat caagacgtcc
      901 aggtgacatt gctgagtgtt ggtcagaccc caaaaaagcc ttcttagaat taggttggaa
      961 ggcgatgtat aacttggacg atatggtaag agatgcatgg aattggcaga aaaaaaatcc
     1021 tagtggttat aaataaaaat actattatcg gttcataacg gatttatatt aatctcagca
     1081 atgtttatgt attgtatgtg aaaatcccct gacaggagta aacaatgtca aagcaacaga
     1141 tcggcgtagt cggtatggca gtgatggggc gcaacctagc gctcaacatc gaaagccgtg
     1201 gttataccgt ctctattttc aaccgctccc gtgagaagac ggaagaagtg attgccgaaa
     1261 atccaggcaa gaaactggtt ccttactata cggtgaaaga gtttgttgaa tctctggaaa
     1321 cgcctcgtcg catcctgtta atggtgaaag caggtgcagg cacggatgct gctattgatt
     1381 ccctcaagcc gtacctcgat aaaggtgaca tcatcattga tggtggtaac accttcttcc
     1441 aggacaccat tcgtcgtaac cgtgagcttt ctgccgaagg ctttaacttc atcggtaccg
     1501 gtgtttccgg cggagaagaa ggcgcgctga aaggtccttc cattatgcct ggtgggcaga
     1561 aagaagccta tgaactggtt gcgccgatcc tgaccaaaat cgccgcagtg gctgaagacg
     1621 gtgagccatg cgttacctat attggtgccg atggcgcagg tcactatgtg aagatggttc
     1681 acaacggtat tgaatacgga gatatgcaac tgattgctga agcctattct ctgcttaaag
     1741 gtggcctgaa cctcaccaac gaagaactgg cgcagacgtt taccgagtgg aataacggtg
     1801 aactgagcag ctacctgatc gacatcacca aagatatctt caccaaaaaa gatgaagacg
     1861 gtaactacct ggttgatgtg atcctggatg aagcggctaa caaaggtacc ggtaaatgga
     1921 ccagccagag cgcactggat ctcggcgaac cgctgtcgct gattaccgag tctgtgtttg
     1981 cacgttatat ctcttctctg aaagatcagc gtgttgccgc atctaaagtt ctctctggcc
     2041 cgcaagcaca gccagcaggc gacaaggctg agttcatcga aaaagttcgc cgtgcgctgt
     2101 atctgggcaa aatcgtttct tacgcccagg gcttctctca gctgcgtgct gcgtctgaag
     2161 agtacaactg ggatctgaac tacggcgaaa tcgcgaagat tttccgtgct ggctgcatca
     2221 ttcgtgcgca gttcctgcag aaaattaccg atgcttatgc cgaaaatccg cagatcgcta
     2281 acctgctgct ggctccgtac ttcaagcaaa ttgccgatga ttaccagcag gcgctgcgtg
     2341 atgtcgttgc ttatgcggta cagaacggta tcccggttcc gaccttcgcc gctgcggttg
     2401 cctattacga tagctaccgt gccgctgttc tgcctgcgaa cctgatccag gcacagcgtg
     2461 actatttcgg tgcacatact tataagcgca ttgataaaga aggtgtgttc catactgaat
     2521 ggctggatta atctgattta accaacagta aaattgaggc ccggcgtata ttgcaccggg
     2581 ctttttttgc catttatctt atagactaaa tttcactgct taatattaac ttaataaata
     2641 tcagctattc ttataaagaa aatctgaatt gtttttcgta gcgttgacca gctaataacg
     2701 tagcgttaaa acatttagct cttatcagga tgctaaaaac atcatgattc acagttaagt
     2761 taattctgag agcatgaaat gaaaatcacc atttccggta ctggctatgt aggcttgtca
     2821 aacgggcttc taatcgcaca aaatcatgag gttgtggcat tagatatttt accgtcacgc
     2881 gttgctatgc tgaatgatcg gatatctcct attgttgata aggaaattca gcagtttttg
     2941 caatcagata aaatacactt taatgccaca ttagataaaa atgaagccta ccgggatgct
     3001 gattatgtca tcatcgccac tccaaccgac tatgatccta aaactaatta tttcaataca
     3061 tccagtgtag aatcagtaat taaagacgta gttgagataa atccttatgc ggttatggtc
     3121 atcaaatcaa cggttcccgt tggttttacc gaagcgatgc ataagaaata tcgtactgaa
     3181 aatattattt tctctccgga atttctccgt gaaggtaaag ccctttacga taatctccat
     3241 ccgtcacgta ttgtcatcgg tgagcgttca gaacgcgcag aacgttttgc tgcgttgtta
     3301 caggaaggcg cgattaagca aaatatcccg accctgttta ccgactccac tgaagcagaa
     3361 gcgattaaac tttttgcaaa cacctacctg gcgatgcgcg tggcgtactt taacgaactg
     3421 gatagctacg cagaaagttt aggtctgaat actcgccaga ttatcgaagg cgtttgtctc
     3481 gatccgcgta ttggcaacca ttacaacaac ccgtcgtttg gttatggtgg ttattgcttg
     3541 ccgaaagata ccaagcagtt actggcgaac tatcagtctg tgccgaataa cctgatctcg
     3601 gcaattgtcg atgcaaaccg cacgcgtaag gattttattg ccgatgccat tttgtcacgc
     3661 aaaccgcaag tggtgggtat ttatcgtctg attatgaaga gcggttcaga taacttccgt
     3721 gcgtcttcta ttcaggggat tatgaagcgt atcaaggcga aaggtgttga agtaatcatc
     3781 tacgagccag tgatgaaaga agactcattc ttcaactctc gcctggaacg tgatctcgcc
     3841 accttcaaac aacaagccga cgtcattatt tccaaccgta tggcagaaga gcttagggat
     3901 gtggcagata aggtctacac ccgcgatctc tttggcagcg actaacatct tgttatcagg
     3961 gctatttacg ccctgattgt cttttgttac ctccgcaata attcattatt tttatcactt
     4021 atcctatagc attcacgggg attatcgcta aactatgcgg acttggaaat ttccgtcagt
     4081 tagggtaatg atgagagtag aaaataataa tgtttctggg caaaaccatg acccggaaca
     4141 gattgatttg attgatttac tagtgcagtt gtggcgtggc aagatgacaa tcatcatttc
     4201 cgtcattgtg gctattgctc tggctattgg ttatttggca gtagcgaagg agaaatggac
     4261 gtcaacagca attatcactc agcccgacgt ggggcaaatt gctggctata acaatgccat
     4321 gaatgttatc tatggtcagg ctgcaccgaa agtatcggat ttgcaggaga cgttgattgg
     4381 tcgcttcagt tctgccttct ctgcattagc agaaacgctg gataatcagg aagaaccaga
     4441 aaaacttacc atcgaacctt ctgttaagaa ccagcaatta ccattgactg tttcttatgt
     4501 tgggcaaact gcagagggag cacaaatgaa gttggcccaa tacattcagc aagttgatga
     4561 taaagtgaat caagagctag aaaaggatct caaagacaac attgctctgg gacggaaaaa
     4621 cttgcaggac tctttaagaa cgcaggaagt agtcgcgcag gagcagaaag atctgcgtat
     4681 ccgtcagatt caggaagcgt tgcagtatgc gaatcaggcg caggtgacaa agccacaggt
     4741 tcagcagact gaagatgtga cacaagatac gttgttcctt ttggggagcg aagcgctgga
     4801 gtcgatgatt aagcatgagg cgacccgtcc gttggtgttc tcgccaaact actatcagac
     4861 tcgtcaaaac ctgctggata tcgaaagctt aaaagttgat gatcttgata ttcatgctta
     4921 ccgctatgta atgaaaccga cgttacctat tcgtcgcgat agcccgaaaa aggcaattac
     4981 cttgattctg gcggtgctgc tgggcggcat ggttggcgcg gggattgtgc tggggcgtaa
     5041 cgctttgcgt aattacaacg cgaagtaatc ttttcggttt taaagaaaaa gggcagggtg
     5101 gtggcacctt gcccgttttt gttgccggat gcgacaacaa catcgcatcc gcttaccccg
     5161 caactcactg atgccgttta cgcaggtttt caattaccgt cgttaaatcc agatcctgat
     5221 cctgcaacag caccagcagg tgatacatca aatcagatgc ctcgttggtc agctcaaagc
     5281 ggtcatgtac cgtcgctgcc agtgcggttt ccacgccttc ttcacctact ttctgcgcaa
     5341 tgcgtttggt gccgctggca tacagtttgg cggtgtagga ggtttccggg tcggcagatt
     5401 tgcgttcggc gagcagttgt tccagttgat acaggaacag ccattggtga gcggtgtcgc
     5461 cgaagcagct gctggtgcct ttgtggcagg ttggtccgat aggattcgcc agtaccagta
     5521 gcgtgtcgtt gtcgcagtcc ggcgcaatat ttactacgtt gaggaaattg cccgacgttt
     5581 cgcctttggt ccacagtcgc tgtttagtgc gcgagaagaa ggtgactttg ccgctttcga
     5641 tggttttgtt taaggcttcc gggttcatat agcccagcat taacacttca ccggataccg
     5701 catgttgcac aatcaccggc atcagtccgt cggttttttc ccagtccagt tcgcggcgtt
     5761 gttgttctgt taacatatcc tgatctccac gccctgtgtt gccaggtacg cttttaattc
     5821 accaatattg attatttgtt tgtggaatac ggaagccgcc agcgcgccgt caacgtcggc
     5881 atcgcggaag gcttcgagga agtgttccat ggtgcccgcg ccaccggagg caatcagcgg
     5941 gacgtggcaa acttcacgca cttttttcag ctgttcgagg tcgtaaccgt tacgcacgcc
     6001 gtcctgattc atcatgttga ggacgatttc tccggcccca cgtttttgca cttcctgtac
     6061 ccagtcgagt gtttcccact gagtgacgcg ggtgcggctt tcatcgccgg tatattgatt
     6121 cacatgatat ttaccggttt cggcgtcgta ccaggtatca atacccacca caatacactg
     6181 cacgccaaag cgatccgcca ggcgagtaat taatgtcggc tccgccagcg caggggagtt
     6241 gatggaaatt ttatccgcgc caaaggaaag aattttcgcg gcatcttcca gagacttaat
     6301 cccacccgcc acgcagaacg gaatgtcgat cacttccgcc acgcgagata cccagctttt
     6361 atctaccaca cggccatcgc tggaagcggt gatatcgtag aacaccagtt cgtctgcacc
     6421 ttcctcggcg tagcgttttg ccagcggcac gatatcgcca atgatttcat ggttgcgaaa
     6481 ctgtacgcct ttcaccacct gaccatcacg aacgtcgaga catggaatta tgcgttttgc
     6541 cagcatgcga tggcctcctt cacggtgaat ttaccttcca gtaatgcacg accaactatt
     6601 acgccgcgca cgccagtgcc acgcagggca gcaacatcgt taatgtcgcc aatgccgccg
     6661 gatgactgaa atgccacctg cgggtatctg gcgcacactt cttcatataa agagacgtta
     6721 gagcctgcca gcgtgccgtc gcgcgagata tcggtacaca gcacatgttt caggccgacg
     6781 ggcaaatagg tttccaccag ttgttccagt gaaacgcccg agttctcttg ccagccgctg
     6841 actgccacct gcttgttgcc ttgctcgtca atacggacat ccagcgccag cactaaggca
     6901 tcggcaccga aacgttcaaa ccagcctttc accatttctg gtgatttcac cgcggtggag
     6961 ccgaccacca cgcgcgcaac gcccgcttcc agtaacgccg ccacgtcttc ttcgctacgc
     7021 acgccgccgc caacctgcac cggaacgtta acgcccgcga ccagggtttt aatcagcggg
     7081 atttgacgtt tagccggatc ttttgccccg gtcagatcca ccagatgcag cacttcggca
     7141 ccctgcgcgg cgtaatcctg taagcgcggc agcgggtcgt taccgtaatc gcgctgtttg
     7201 ccgtaatcgc cctgatggag acgcaccacg gtgccgtcga ttaaatctaa tgccggaata
     7261 atcatcacat ctccaggaag tttttcagca acttagcgcc agcggcacca gaacgctccg
     7321 ggtggaactg cacgccgtag aagttatctt tttgtaccgc cgcggtgaac ggttcgccgt
     7381 aattacactg ggcgatggtc cacggattga ccggcattgc gtagctgtga acaaagtaaa
     7441 agtacgcgcc gtcttcaatc ccctgaaaca ggcggttgcc tgcctgcggg taaacgcggt
     7501 tccagcccat atgtggcagt ggcagaccaa agtcggtcat tttcggcacg tcttcgtcga
     7561 tgatgcccag caaatcgacg ccgttgctct cttcgctgcg ccgcccaagc agttgcatcc
     7621 ctaagcagat gcccagcacc ggttgggtac aggctttgat gagatcaaac agttcgcgct
     7681 cacgcacctg atccatcgct gcttgtgcgg tgcctacgcc gggtaaaaat agtttatcgg
     7741 ccagcaacac gacgtccggg tcacggctga ctttgggttc ataaccgtgg cgcgtaatgg
     7801 ctgacttcac cgagttcaaa ttggcgcagc cggtatcaag gatcaccacg ttcattacag
     7861 cactcctttc gacgagggca gggtgtcgcc ttccacgcgg atggcctggc gcagggtgcg
     7921 accaaaggct ttgaacaggc tctctacacg gtgatgatcg tttttacctt tggttttcag
     7981 gtgcagggtc acgcccatgg tgtatgagag cgaacggaag aagtgctcga tcatttcggt
     8041 gctgagatcg cccacgcgct ggtaggtaaa ttcggcctta tattccaggt gcgggcgacc
     8101 agagatatcc agtgcacagc gggcaaggca ttcgtccatc ggtagcacaa aaccaaagcg
     8161 acaaataccg cgtttatcgc cgagggcaat ttttaacgct tcgcccagcg ccaggccggt
     8221 atcttcgacg gtgtggtgat cgtcgatata gaggtcgcct ttgacgttga tttccatacg
     8281 gaagccgccg tgggtggcga tctgatccag catgtgatca aagaagccaa cgccggtgtt
     8341 aatcttgctg ccaccttcac gatccagcca cacctggacg tcaatttgcg tctctttggt
     8401 gttgcgcact acatgggcgt aacggtctcg tttagtgagt tgctcgccaa tcatcggcca
     8461 gttcagggtt tcgcggtcgt agcgtaaacc attaatgccc atgttttccg ccagttgaat
     8521 atcggtcgcg cgatcgccaa tcacataact attggcacga tccatcgctt gctcagccag
     8581 ataacgctcc accagtttta ctttcggctt acggcattcg cactcatcgg cgggcaggtg
     8641 cggacaaatc agcacttcat caaactgcac gccttgcgag gtgaagatct gcatcatcag
     8701 gttgtgcggt ccatcaaaat ccgcctgcgg gaaactttgt gttcccagac catcctgatt
     8761 agtgatcatc accagcttgt agcccgcttt ttgcagcttt agcagctccg ggatcacgcc
     8821 cggttcaaag gcgagtttgt caaaacggtc cacctgaaaa tcactcggcg gttcgctaat
     8881 cagggttcca tcgcgatcga taaaaagata cttctgactc atcaaacttg ctccgcacgt
     8941 aaggcgtcaa tgacgcgctg gctttcttca tgggttccga cggtaattcg caggcagccg
     9001 cttaaagagt gttgtttatt ctgatcacgt aagataatgc cctgatccca caaagattta
     9061 aacactgcac tggaggcttt aaagcgcgcc agaatgtagt tggtttcaga gtcaaaaacc
     9121 tgctccacgc aggggatctc tttcagggcg gcaatcaggt attcgcgttc tgcaataatt
     9181 tgcgccaccc gttcgcgcat ggcgacgatc ccctgcgggc ttaacgcctg ggccgcaatg
     9241 tcggcaaccg gcgtcgagag cgggtagggg gcgatcactt tcatcagcag gttgatgagt
     9301 tcttcgtttg ccagcgtaaa cccgcaacga aggcccgcca gagcaaaagc tttcgacagt
     9361 gtgcgcaaaa tagccaggtg cggatattcc gccagccagc cagccagcga tgcctgcggg
     9421 caaaactcga tataggcttc atcggcaacc acaatcgctt taccgcgggt taactccagc
     9481 agagtgcgaa aatcctgtgg attgatcagt tgcccggtgg ggttgttggg gctgcaaaca
     9541 tagaccactt ttacgtcgtc cagcttgtcg gaaatgccct gtaagtccag ttgccagttg
     9601 tccagtgtcg gcactgtgcg gcactcgacg ccaatggttt cggcgctgac gctgtacatg
     9661 ccgtacgttg gcgggcagta gaggatggcg tctttacccg gttcgcaaaa agcgcgaatc
     9721 agcagttcaa taccttcgtc cgcgccacgg ctgaccagca cctgttccgg ttttacgcct
     9781 gcatattgcg cgtaattttc aatcaccgct ttcggctgac attccgggta gcggttgagc
     9841 gtttgctgag taagctgaaa ctccacggct gtgggatatt cgttggcgtt cagccagacg
     9901 tcgccgttac cgcccagacg acgcgccgac tgatacggcg tcaggttgcg gacgttttca
     9961 cgcgctaaat cggtaatagt cacggtgctc atgcttgctc cttaagggcg ttaacacgca
    10021 aagtaacggc atttttgtgg gcggtcaggc gctcggcggc ggccagtgtt tcaatggtcg
    10081 aagccagcgc ggagaaacct actttcgaca gttcctgcac ggtcatccgc ttctggaaat
    10141 ccgccagtcc gaggctggaa caggtggcgg tgtagccgta agtcggtaga acgtggttgg
    10201 tgccggaggc gtaatcacct gccgattccg gtgaccagtc accaagaaat accgaaccgg
    10261 cactggtaat gccatcgacc agttcgcggg cgttgcgggt ctgaatgatc aggtgctccg
    10321 ggccgtactg gttggagatc tccacgcact gtgctaaatc gttggtcacg attaggcggc
    10381 tggcgttcag tgcctggcgg gcggtttcgg cacgcggcag ttctgccaac tggcgttcga
    10441 cggcctcggc aacgcgacgc gccatatcag cagcgggcgt cagtaaaatc acctgtgagt
    10501 ccgggccgtg ttcagcctga gaaagcaaat cagaagccac gaaatctggc gtagcgccgc
    10561 tgtcagcaat caccagcact tccgacgggc ctgcgggcat atcgatcgcc gcaccgtcca
    10621 gacgctggct cacctgacgt ttcgcttcgg tgacaaaggc gttacccggc ccgaagattt
    10681 tgtccacttt cggcacagat tcagtaccaa acgccagtgc ggcaatggcc tgtgcgccgc
    10741 cgacgttaaa cacatcctgt acaccgcaca gctgcgccgc ataaaggatc tcatcggcaa
    10801 tcggcggcgg tgagcacagc accacttttt tacagcccgc aatacgcgcc ggagttgcca
    10861 gcattaatac cgttgagaag agcggggcgg agccgccagg aatatacaac ccaactgaag
    10921 cgactgggcg cgtgacctgc tggcaacgta cgcctggctg agtttcaaca tctaccggcg
    10981 gcagtttttg cgcagtgtgg aaggtttcaa tattctttac tgccaccgcc atcgcctgtt
    11041 ttagctcgtc gctcaggcgt ccgctggcgg cggcaatttc ctcagcagac accttcagtg
    11101 cggtaaccgt ggttttatca aacttcgcgc tgtattcccg cagggcctca tcgccgcgtg
    11161 ctttcacgtt atcgagaata tcgttaacag tacgggtaat gctttcagaa gcggagatcg
    11221 ccgggcgcat taacagctgg cgttgttgct ccgcagtaca gctattccag tcaatgattg
    11281 tgttaaagct catggcgatc actccatcat cttctcaatc ggcaggacca ggattgaact
    11341 ggcacccagc gctttcagtt tttccatcgt ttcccagaac agggtttcgc tactcaccat
    11401 gtgcatcgct acgcgctgtt gatcacctgc cagcggcaga atagttgggc gttcggcacc
    11461 tggcagcagg gcgatgactt catccagacg ttcagtcggt gcgtgcatca tgatgtattt
    11521 tgattcgcgc gcctggatca cgccctgaat acgggtcagc agcttgtcga tcagttgctg
    11581 tttagattct tccatttcgc catcgcgctg gatcaggcag gctttcgagc ggtagataac
    11641 ttcgacttcg cgcaggccgt tagcttccag cgtggcaccg gtggaaacca gatcgcaaat
    11701 cgcatccgcc agtccggcgc gcggggcgac ttcaacagaa ccgttcagta agcaggattt
    11761 aaaagagatg cctttctggt cgagataacg cttgagcagg tgaggataag aggtggcgat
    11821 acgtttaccg tttaaggaga gcggaccgtc ccaggcttca tcaaccggcg ttgccagcga
    11881 aaggcggcag ccgccgaaat ccagtcgacg cagggtaaag tagcgcggat cttcaccctg
    11941 ggctcggcgg ttaagcagct cttcttccag cacgttttcg ccgataatcc caaggtctac
    12001 cacgccgtcc attaccagac cgggaatgtc gtcgtcacgc acgcgcagaa tatcaatcgg
    12061 catgttttct gccatcgcga tcaggcgctg ggtgtgaaga ttgattttaa tgccacagcg
    12121 cgccagcaat tcgcgtgagt catcacttaa acggccggat ttctgcatag ctatgcgtaa
    12181 acgagtgttg tctgtcattt tcttattcct ctttaaacct gtctgaaccg ttatcaaccg
    12241 cgcaataaaa aagcccccgg aaggtgatct tccgggggct ttctcatgcg ttcatgcacc
    12301 actggaagat ctgaatgtct tccagcacac atcgcctgaa agactagtca ggatgatggt
    12361 gatgatggtg gtgtttaaat tgaacgcgtg tcataaaagt ctctgtgaat gtttattcaa
    12421 ctgatgtctt ttaacctaaa ccactttcac gttagaaagc aagggctttt ttatatattt
    12481 tatggcgatg gatttgttta atgaatgagc ggggaaagga gggggctaag aggagtgtcc
    12541 cctaacctga cggcacccaa aatagcgaaa cctggatttt tcgtctcact gacaatgtct
    12601 tgatctacag actaattaat aaatagttaa ttaacgccta tcattgtaca atgaactgta
    12661 caaaagagga gattgaaatg cgtacaatta gctacagcga agcgcgccag aatttgtcgg
    12721 caacaatgat gaaagccgtt gaagatcatg ccccgatcct tattactcgt cagaatggag
    12781 aggcttgtgt tctgatgtca ctcgaagaat acaactcgct ggaagagacg gcttatctac
    12841 tgcgctcccc cgctaacgcc cggagattga tggactcaat cgatagcctg aaatcaggca
    12901 aaggaacgga aaaggacatt attgagtgaa actaatctgg tctgaggagt catgggatga
    12961 ttatctgtac tggcaggaaa cagataagcg aattgttaaa aagatcattg aacttatcaa
    13021 agatacccgc agaacaccat ttgaaggtaa ggggaagcca gaacccctga agcataattt
    13081 gtcaggcttc tggtcccgac gcattacaga ggagcaccgt ctggtatacg cggttaccga
    13141 cgattcactg ctcattgcag catgtcgtta tcattattga acagattcat tcatagaatg
    13201 aattgtcagt tttgatacgc tagcgtagcc ttatagatct aaggtgtatc aggagataac
    13261 ggatgaaaaa ggtcgcaatt gtcgggttag ggtggttagg catgccgctg gcgatgtcac
    13321 tttcagcgcg aggctggcaa gtcaccggga gtaaaaccac gcaagatggt gtcgaagcgg
    13381 cccgaatgag tggcattgat agttatctgc ttcgcatgga gcctgagtta gtttgcgatt
    13441 ctgacgatct ggatgccctg atggataccg atgcgctggt cattacgctt ccggcacgtc
    13501 gtagcggccc cggcgatgag ttctatttac aagcggtaca agagttagtg gatagcgcgc
    13561 tggcccatcg tattccccgc attattttca ccagctcaac atctgtctat ggcgatgcgc
    13621 aaggcacggt gaaagaaact accccgcgta atccggtgac caacagtgga cgagtattag
    13681 aagagctcga agactggctg cacaatttac ccggtacttc ggtcgatatt ctgcgtcttg
    13741 cggggctggt cggaccggga cgtcatcccg gacgcttctt tgccggaaaa accgcccctg
    13801 atggtgaaca tggtgttaat ttagtccatt tagaagatgt tattggcgct atcactctgt
    13861 tgttacaggc acctaaaggc ggacacatct ataatatatg tgcgccagct caccctgcgc
    13921 gtaatgtttt ctatccgcag atggcccgtt tactggggct ggaacctccg cagttcagaa
    13981 atagtctgga cagcggcaaa ggtaagatta ttgatggcag tcggatttgt aatgaactgg
    14041 gatttgaata ccagtatccc gatccgctgg taatgccgct ggagtaaacg atcacgccag
    14101 caggacgccg catgcactgc aagggggcgt gtatgaaacc actgctggat gtgttgatga
    14161 tcctcgatgc cttagaaaaa gaaggcagtt ttgcggcggc gtcggccaaa ctctacaaga
    14221 cgccgtccgc tctgagttac accgttcaca agttggaaag cgaccttaat attcaattgc
    14281 ttgatcgtag cggacaccgc gctaaattca cccgcaccgg aaaaatgtta ttagagaaag
    14341 ggcgggaagt tctgcatacc gtgcgggaac tggaaaagca ggcgatcaaa ctgcatgaag
    14401 gctgggaaaa cgagctggtg attggtgtgg acgacacctt ccctttttct ctgcttgcgc
    14461 cactcattga agctttttat caacatcata gtgtgacgcg cctgaagttt atcaatggcg
    14521 tactcggtgg ttcctgggat gccctgactc aggggcgagc ggatattatt gttggcgcga
    14581 tgcatgagcc accatcttcc agtgagtttg gtttctcgcg gttaggcgat ctggaacaag
    14641 tctttgcggt cgcgccacat catccgttag ccctggaaga agaaccgtta aaccgtcgta
    14701 tcattaagcg atatcgggcg attgtggtgg gggatactgc gcaggcaggc gcttctacgg
    14761 cctcacaact tctcgacgaa caagaagcca ttaccgtttt cgattttaaa accaagctgg
    14821 agctgcaaat tagcggcctt ggctgcggct atttaccccg ttatctggcg caacgttttc
    14881 tcgatagtgg cgcgttaatc gagaagaaag tggttgccca aactctcttt gaacccgtct
    14941 ggattggctg gaacgaacag accgcagggc ttgccagcgg ctggtggcga gatgaaattt
    15001 tagcaaatag tgcgatcgcc ggtgtttatg caaaatctga tgacggaaaa tcagccattt
    15061 aaagaaaaat tattatgaca agcctctcat tctcttgtca tttccccccc atttaggcac
    15121 aatgcgccgc tgtcaaaaaa tgactaaaaa ccgacgtttc atcagcgtcg gttatttttt
    15181 gcttcaaacc aatcattcat accaagaggc cgggcttcgt accggataga tatttactaa
    15241 aaatcgacag ttgttgtcgc tgaggaatcc aaaaaaatgg ggcaattttt tgcttacgcg
    15301 acggttatca ccgtaaagga gaatgaccat gtcgcataac gttactccaa acacctctcg
    15361 cgtggaattg cgtaaaacgc ttacgttagt tccggttgta atgatgggtc ttgcctatat
    15421 gcagccgatg acgctgtttg atacttttgg tatcgtttca ggcctcacgg atggtcatgt
    15481 gccgacagcc tatgcgttcg cattgattgc gatcctgttt acggctctga gctacgggaa
    15541 gctggttcgc cgctatcctt ctgctggctc tgcatacact tacgcccaga aatccattag
    15601 cccgaccgtc ggctttatgg tgggttggtc ttctctgctc gactatctgt tcgcgccgat
    15661 gatcaacatt ctgctggcga aaatctattt tgaagctctg gtgccttcca ttccatcgtg
    15721 gatgtttgtg gtggcgctgg tggcttttat gaccgccttt aacctgcgta gtctgaaatc
    15781 cgtagcgaac ttcaacaccg tcatcgtggt gttgcaggta gtgctgatcg cggtaattct
    15841 gggcatggta gtttacggag tatttgaagg tgaaggcgcg ggtacgctgg cgagcactcg
    15901 tccgttctgg tctggcgatg cgcatgttat cccgatgatt accggggcga caatcctgtg
    15961 tttctccttt accggctttg acggcatcag caacctgtcg gaagaaacca aagatgcaga
    16021 gcgcgtgatc ccgcgtgcga ttttcctgac cgcgctgatt ggcggcatga tcttcatctt
    16081 tgcaacttac ttcctgcagc tgtacttccc ggatatctct cgctttaaag atccggatgc
    16141 gtcacagcct gaaatcatgc tgtacgttgc gggtaaagcg ttccaggttg gcgcgctgat
    16201 cttctccacc attaccgtac tggcgtccgg tatggcggcg cacgcaggcg tagcgcgtct
    16261 gatgtacgta atgggtcgtg acggcgtgtt cccgaaaagc ttcttcggtt atgtacaccc
    16321 gaaatggcgt actccggcga tgaacatcat cctggttggc gcgattgccc tgctggcaat
    16381 caacttcgac ctggtaatgg ctacggcgct gattaacttt ggtgcgttgg tggcgttcac
    16441 cttcgttaac ctgtcggtta tctcgcagtt ctggatccgt gagaagcgta acaagacgct
    16501 gaaagatcac ttccagtatc tgttcctgcc gatgtgtggt gcgctgaccg ttggtgcgct
    16561 gtgggttaac ctggaagaaa gctcaatggt tctgggtctg atctgggctg ctatcggtct
    16621 gatttacctg gcttgcgtca ccaagagctt ccgtaacccg gttccgcagt acgaagacgt
    16681 agcataatca tcttttctaa taatcaaccg gagtcgaatg aatatttggc tccggttttt
    16741 ttattctttt ttatcgaaat cctcattaat aaccatttgt tctaatttag agaatttggt
    16801 tattggtctg cattccacca gtcatgaata atttcctcat taacattcat ctggttaatt
    16861 aatttatgtt ttcaatgata ttaagcgggc taatttgtgg tgctctgctg ggatttgtca
    16921 tgcagcgtgg gcgtttttgc ctgacaggtg gttttcgcga tatgtatatc gcgaaaaata
    16981 atcgcatgtt ttacgccttg ctgattgcaa tttcggtaca aagcgtgggg gtttttgcgt
    17041 taattcaggc gggcctactg acttacgaag ccggggcgtt cccgtggctt ggtactgtta
    17101 taggtgggta tcttttcggg ctgggaattg ttctggcggg tggatgtgcc accgggacct
    17161 ggtatcgcgc gggtgaggga ttgatcggca gttggatcgc gcttttcact tatatggtga
    17221 tgagtgcggt gatgcgttct ccacacgcca gtggtttaaa tcaaaccttg cagcactaca
    17281 ctactgaaca taactctatt gctgatactt ttaatttgtc tgtgtggccg ttggttgccg
    17341 ttttgctggt gataacgctc tgggtggtga tgaaagagtt gaagaagcca aaactcaaag
    17401 tcgcgacctt accgccgcgc cgaaccggga tcgctcatat tctgtttgaa aaacgctggc
    17461 atccctttgt cacagctgta ctcatcggtt tgattgcgct tttagcctgg cccctgagtg
    17521 aagcaaccgg acgtatgttt gggttaggca tcacttcccc aacggccaat attctgcaat
    17581 ttctggtcgc gggtgacgtg aaatacatta actggggcgt tttcctggtg ttagggatct
    17641 tcgtggggtc atttattgcc gccaaagcga gccgtgagtt ccgcgttcgc gctgctgatg
    17701 cacaaacaac attacgtagc gggttaggtg gtgtactgat gggcttcggt gccagcattg
    17761 caggtggttg ctctatcggt aatggactgg tcatgactgc aatgatgacc tggcagggct
    17821 ggattggcct tgtatttatg attctcggag tctggactgc gtcctggctt gtgtatgttc
    17881 gaccgcagcg taaggcgcga ctggcaactg ctgcggcaaa ttaagtaagg tgtaaagatg
    17941 gcgattaaaa agctggatgt ggtgacgcag gtctgtccgt ttccactcat agaagcaaaa
    18001 gcagcactgg cagaaatggc cagcggcgat gagttggtaa ttgagtttga ttgcacccag
    18061 gcaactgaag caatcccaca gtgggcggca gaagaaggac atgccatcac cgattatcag
    18121 cagattggcg atgcagcgtg gagcatcacc gttcaaaaag cctgactcaa aattgtcctc
    18181 cgccgtacca gcggcggagg cttcacatta gacaatctct tccgcgtact gccaaagcgc
    18241 ttttaacagc gccacttttt ctttatcgtc ggcatactgc tgcgccagca tctgaatctc
    18301 ttctgcatag ccttgtaaaa actctggcgt gaagacctgg cggcggtgct ccagccagcg
    18361 ttgctgctcg gcataatcca gcgtccccgg gaagttgcgc gcccgataat tgaacaacag
    18421 cttttcaatc cgtttatcaa caaaagtgat atccagtgcc ggtaaattac gcggctcggt
    18481 ttcaagcaca attttcattg ctgcacgatc ggcgtcactg aaaaagccat tataaagctg
    18541 tgcatccacg ttatctgaag gcgtaaacgg ttcggcttcc gcgaatatcg ccaccacttt
    18601 ttcacgtact tgcggatttt cacgcagaat ttttaggtta tcgaggcaat gctgacgatt
    18661 aattcccagt cggtcggcat cttccgggcg tagcgtattc gcctgggcca gcaccgggca
    18721 tttatttatg tgcaccagct taaccggaac ggcggcgtta tcgccaagat cggtttttgc
    18781 ggtatataaa cgctcgcgca atgtgtcgct atccagttcc agtaatggcg aaatgtctcc
    18841 tgccaaatcc accataatta cggcattgcg attttcagga tgccacgcca gcggtgctac
    18901 ccagctggta ttaccgcgcc atgccccaaa cattccggaa acgtgaacca ggggtttcat
    18961 ctgcggaaca tcaatcaacg ccatcagttt gtgtttattg cgatgggtaa agagataatc
    19021 aaacaggcgt ggctgacgcg tttttaccag tttcgccatc gcaatagtgg cgtacacatc
    19081 agccatcgca tcgtgggcgt tgctatgttc aataccattc gctttggtta aatgctcaag
    19141 gcgaaagctc ggtagaccgt catcattttc aggccagttt attccttccg ggcgcagggc
    19201 ataacaggca cgcataacat ccagtaaatc ccagcgcgag ttatcatgct gccagctcca
    19261 ggcgtaagga tcgtagaaat tacgataaaa aacgttgcgt gtgacttcgt cgtcgaaacg
    19321 cacattgttg tagcccagaa tacaggtctt cggtacggta aaaagcgagt gaatacgggc
    19381 ggcaaatgcg gcttcgtttt ctcctttcgc ccgagcttcc tgcggggtaa taccggtaat
    19441 taatactgct ccaggctggg gtaaatagtc atccgcgggc ttgctgtaaa agacttcggg
    19501 ttcgccgatg acattgaatt cgctatcggt gcgaatggct gcgaactgtg cagggcgatc
    19561 taacgcgggg tgcgtgccaa aggtttcgta atcgtgaaac aaaaaggtag attgttgctt
    19621 accgtcattc atcattaggt taaatccgtt atttctgctg tctgccagag tatcaaatat
    19681 caccgtgcta atcagcttta gcgccacaat ttggcagcga gtggcaacag atcatgtcag
    19741 ataaaaatga gagggtagtc acattttctt gcactttatt ccagccagtt cataagtatt
    19801 tccgtaaaaa gaacagctat ttgaaactcc tgagggtttg ctgttgaaac gccgtcttat
    19861 tattgctgct tctttgttcg tttttaactt atcgtctggt tttgcggcgg aaaacattcc
    19921 tttttcacct cagcctccag agattcatgc cgggtcctgg gtattgatgg attacaccac
    19981 cagtcagatc ctcaccgcgg ttaatgagca tcaacagcgc aatcccgcca gcctgacaaa
    20041 gctgatgacg ggttatgtcg tggatcgcgc tatcgatagt catcgcatta cgccagacga
    20101 tattgtcacc gtggggcgcg atgcgtgggc gaaagataat ccggtgtttg tcggttcttc
    20161 actgatgttt ttgaaagagg gcgatcgcgt atcggtacgt gatttaagcc gtggtttaat
    20221 tgtggattcc ggaaatgacg cttgtgttgc tctggctgac tatattgccg gtgggcaacg
    20281 gcagtttgtt gaaatgatga acaactatgc cgagaagctg catctcaagg atacgcattt
    20341 tgaaacagtg catggtctgg atgcacctgg ccagcatagc tcggcttatg atttagctgt
    20401 gctttctcgc gctatcatcc acggcgagcc cgagttttat catatgtaca gtgagaaaag
    20461 tctcacctgg aacggtatca cccagcaaaa ccgtaacggg ttgttgtggg ataaaaccat
    20521 gaatgttgac ggcctgaaaa cgggtcatac ttctggtgcc gggtttaatc tcattgcttc
    20581 ggctgtagat gggcagcgtc gtctcattgc agtggtaatg ggggctgaca gtgcaaaagg
    20641 tcgtgaggaa gaggcaagaa aattactgcg ttgggggcaa caaaacttta ctacggtgca
    20701 aattttgcac cgtgggaaaa aggtcggaac ggaacgcatc tggtatggcg ataaagaaaa
    20761 tatcgacctg ggaacggaac aagagttctg gatggtgcta ccgaaagccg aaattccaca
    20821 tatcaaagcc aaatataccc ttgatggtaa agagctcacc gcgccaatta gcgcccatca
    20881 gcgggtaggg gaaattgaac tttacgaccg tgataaacag gtggcgcact ggccgctggt
    20941 taccctggaa tctgtcgggg aaggcggcat gttttcccgc ctgagtgatt atttccacca
    21001 taaggcctga cctttctttt gcagcagact ggcaggagtg cgagtctgct cgcataatca
    21061 acactcattc cttgtggttt taatattgca actatactgt atataaaaac agtgttaatg
    21121 gaggcgtcat gaactacgag attaagcagg aagataaacg taccgttgca ggtttccatc
    21181 tcgttggccc gtgggaacag acggtaaaga aaggttttga gcagttgatg atgtgggtag
    21241 atagcaaaaa tattgtgccg aaggagtggg ttgctgtcta ttacgacaat ccggatgaaa
    21301 cacccgccga aaaattacgc tgcgacaccg tcgtgacggt accgaataac tttacgctcc
    21361 ccgaaaacag tgagggcgtc attctgacag aaatttcagg tggtcaatat gcggtggcgg
    21421 tggctcgtgt agtcggtgat gattttgcta aaccctggta tcagttcttt aatagcctct
    21481 tgcaggacag tgcttatgaa atgttaccaa agccctgctt cgaggtttac ttgaacaatg
    21541 gcgcggaaga tgggtactgg gatatcgaaa tgtatgttgc ggtgcagcca aaacatcact
    21601 aattcatctc agggcggtgt gttgacgcga agaccactct ttttttgaaa gcgaaaagag
    21661 taagatgcgc ctttcaattt tttcgctcct gccgggaaat tacactgttc ccggtttgtc
    21721 cgtcggataa ttcagaggcg cgccttctgg tcgacagatg agttatgagc gcttttaatc
    21781 ttcattacgg agtttctgcg tgcgtgccga taagtcatta agcccgtttg aaatccgggt
    21841 ataccgccat taccgcattg tgcatggtac tcgggtcgcg ctggcattcc tgctcacttt
    21901 tctcattatc cgcctgttta ctatcccgga aagcacctgg ccgctggtca ccatggtggt
    21961 gattatgggg ccaatctcgt tctgggggaa cgttgtccct cgcgcattcg aacgtattgg
    22021 cggtacggtg ttggggtcga ttttaggtct tatcgctctg caactggagt taatctcgtt
    22081 accgctgatg ttagtctggt gcgcggcggc tatgtttctt tgcggttggc tggcgctggg
    22141 caagaaaccg tatcaaggtt tattgattgg ggtgacgctg gcaattgttg ttggttcccc
    22201 gacaggtgaa attaatacgg cgttatggcg aagcggcgat gtgatcctcg gctctttact
    22261 ggcaatgttg tttaccggta tctggccaca acgggcgttc atccactggc gcattcaact
    22321 ggcgaaaagt ctgaccgagt ataatcgggt ctatcaatct gcattctcac cgaacttact
    22381 cgaacgccca cgtctggaaa gccatctaca aaaactcctg accgatgccg tgaaaatgcg
    22441 tgggctgatt gcgcccgcca gcaaagaaac ccgtattcca aaatcgatat atgaaggtat
    22501 ccagaccatt aaccgcaatc tggtttgtat gctggagttg caaatcaatg cgtactgggc
    22561 cacgcgcccc agccatttcg tgttattgaa cgcgcaaaaa cttcgtgata cccagcacat
    22621 gatgcagcaa atactgctga gccttgttca tgcgctgtac gaaggtaatc cgcagccggt
    22681 ttttgccaat acggaaaaat tgaacgatgc tgtggaagag ctgcgtcagt tgctcaataa
    22741 ccaccatgac ctgaaggtag tggaaacacc aatctatggt tatgtgtggc tgaacatgga
    22801 aacggcgcat cagcttgagt tgctatcgaa tctgatttgc cgggccttgc gcaaataatt
    22861 cctgaacttc agaatcatct tgctgctgct tcgattcagc aaggataaag ggtatgatag
    22921 tgaaaaggga taaaagcatt gtcatctgcg gcagctatga gtaatgttgg ccctaacgaa
    22981 tagcggttgc ttaaacgaat ccgactctca cattatcagg ggtataaaaa tggaaactac
    23041 caagccttca ttccaggacg tactggaatt tgttcgtctg ttccgtcgta agaacaaact
    23101 gcaacgtgaa attcaggacg ttgagaaaaa gatccgtgac aaccagaagc gcgtcctgct
    23161 gctggacaac ctgagcgatt acatcaagcc tgggatgagc gttgaagcaa tccagggcat
    23221 catcgccagc atgaaaggtg actatgaaga tcgcgttgac gattacatca tcaaaaatgc
    23281 cgagctctcc aaagaacgcc gcgatatctc caaaaagctg aaagctatgg gcgaaatgaa
    23341 aaacggcgaa gcgaagtaat tcccgtttta ttcaatgagg gttgcccggc aaccctcatt
    23401 gctcattgat tcttatctgt gtatcaccgt catcattctc atctgagaac caatcgaaat
    23461 taacaacagc cttcttctgt atgcagcaag gcaaaaagtt ctgtaactcc attgttatta
    23521 actgcactgg ttactaacac gttgtgcgct ccagcttccc gtaaccaact tttcaccaaa
    23581 gatatttgtt ccatgctggc taaatctgct ttggttacta ctccaatgac cgggtgattc
    23641 atggcccggt agggcgtttt tacctctaat tcctgcttca gcactgagat tcctgcttgt
    23701 tgccggaaaa gcaggcacct gccgtctgaa cggtatcgat ccggaagcgt atctgcgcca
    23761 tattctgagc atactgccgg aatggccctc caaccgtgtt gacgaactcc tgccatggaa
    23821 cgtagttctc accaataaat aagcgtcaat acggtgctcc gttgacgctt actttaatcc
    23881 gttttctgag cttagatttt tctgttccta cccagaccgt ggatttggaa ttcatttttt
    23941 gtcaggtcaa tgctaatcgt taaaacggcc ataacgcacc tccactggaa ggtttatcag
    24001 taatatccta ccgttgcttc atggtcgggg cgtccattcc attattatcg gttttccttt
    24061 ctgtttataa atgaaaacgc cagctgtatt caggctggcg tcaggggaaa tgaagcctgt
    24121 tgagtgagat tcaccggttc tggtgcagaa gctgcagatg gcgcagggta aggacatctt
    24181 ctcttgcctg cttaatacag gacaacagtg ctgtttctgt ggttaatggc actataccct
    24241 gcatcactgc attgtcttac agcaactggt gagggctggc ataagttgcc ggtgtttcgg
    24301 tagagtcacg gtcatccacc acgcggagtg aataaccgtt ttcagcggcc ttattaatca
    24361 gttcagtgat attaacacca tcaatgtcaa cgacaatgtg ccccatatcc agcgcctgta
    24421 cgttaacgct gtcggcttcc agtgtcatca tttcgcctcc ggatacttac ccagggtaat
    24481 gttatttacc gttctgtaat tgtcgcgggt catcaggccg gtcgccctgc gagcccggag
    24541 gatatcgatg ctgtttatta actgagagcg ggtacaggcg ctgaatcccg gctggtcggt
    24601 agcaccagcg cgtatttttc cacgagaaag ttcaccgcat cacacagtga aatgcctgcc
    24661 tcaatatgct gctcaatcac acgttcatcg gcaaaaggtg tgtcattcag tgtgaggcca
    24721 tagtgctggt ccagcagtcg ggacagcagt atctgccaga tttcaacagg agacgggcga
    24781 gaactggccg cctgcccggg taatacaggt aatgttttca tactgaagat tttcctgata
    24841 tgcagatata aaaatggaaa agtggcgtgg tgaaaacacc aggccgtagc agaaggctat
    24901 tctggagagt taatttttca tttcgggcgt cggataaaca gccagataaa cgtaaccaca
    24961 actgctgagg gtatcggctt tgcaggtcag cccttttgca tacagcgtga cggtatgctg
    25021 atggcgggga ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg
    25081 aaaggcctgg tccaggtggt acgcatctgc atcgctgaac aggcctctga taccggcgcg
    25141 gtcggcaagg taatgcaacc ggttaccctc ctgcaccaga cgtgccccga aacagggcgt
    25201 cacggtgcag ggcagccccc accaggggcg gtcgtgattg tcgtcgggaa gtgttgtccc
    25261 ggggagtgtg tctgacacga taaaatcctt acagaaaatc ggctaagaat ggtccggtag
    25321 tggcgataat tctgctcatc agaattccca ctcagttcag ggtgacgctc atcagccgga
    25381 catacggacc aaaactgtcc ttacggcgtt cagcaaacac ggccagcaca ccgggaatat
    25441 cctgcacttc acgaccggta tacgcttcag cactgccgtg ccagcggtat ttaccggtac
    25501 agaacggaaa aagacgggat gtcggatgtt gttggtgaat acgcatggct tcaccacggg
    25561 tgataatttt cataatggga tacctctgaa gacagaagat aaaagtgaaa acaggtgtga
    25621 tgtggttgtg aagggttaaa gcagaccatg ttcggcaaag gagaaaacct ggttgccacc
    25681 gactatcaga tggtccggca cccggatatc caccaggccc agtgcctgta ccagacgttc
    25741 ggtgataagc cggtctgcct tactgggtgt gacttcaccg gacgggtgat tgtgtgccag
    25801 taccacggca gcggcattgt ggtacagggc gcgtttaatc acttcccggg gatggacttc
    25861 cgtgcggttg atggtgccgg tgaagagggt ttcaccggca atcagctgat tctggttgtt
    25921 cagatacagc acccggaatt cttcacgctc cagtcccgcc atgttcagaa tcagccattc
    25981 ccgtgctgca cgggtggagg tgaaggccac gccgggttca tgaagatggc ggtccagggt
    26041 tttcagggcc cgttgaatga ggctgcgctc gccgggcgtc atctctccgg gcagaaagga
    26101 aagttgttgc attgtgcttc tctccattca gtcgatgatg cgcataatgg cgctgcattc
    26161 cggatgctgc agggcgtaat cccgcaaccg gtaataatgg accgtcatgg cataattctc
    26221 cgtacgacag gcatgatgac tgtacgtcat cagacaggcg gcaataccgg cggcttccgg
    26281 gctcatttct gcgcggttac cgttcatggc attgaacagt acccatgttt cgtcatcatc
    26341 gtcatccggt tcggg
//
LOCUS       DBBGLQ010000058        26128 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.058, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000058 DBBGLQ010000000
VERSION     DBBGLQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.058"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..103
                     /gene="rrf"
                     /locus_tag="ACIYSB_003321"
     rRNA            <1..103
                     /gene="rrf"
                     /locus_tag="ACIYSB_003321"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            430..1728
                     /gene="kgtP"
                     /locus_tag="ACIYSB_003322"
     CDS             430..1728
                     /gene="kgtP"
                     /locus_tag="ACIYSB_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGC5430304.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1725..2048)
                     /gene="yfiM"
                     /locus_tag="ACIYSB_003323"
     CDS             complement(1725..2048)
                     /gene="yfiM"
                     /locus_tag="ACIYSB_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGC5430305.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2094..3449)
                     /gene="pssA"
                     /locus_tag="ACIYSB_003324"
     CDS             complement(2094..3449)
                     /gene="pssA"
                     /locus_tag="ACIYSB_003324"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGC5430306.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3563..6223)
                     /gene="pat"
                     /locus_tag="ACIYSB_003325"
     CDS             complement(3563..6223)
                     /gene="pat"
                     /locus_tag="ACIYSB_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGC5430307.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6255..6953)
                     /gene="tapT"
                     /locus_tag="ACIYSB_003326"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6255..6953)
                     /gene="tapT"
                     /locus_tag="ACIYSB_003326"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGC5430308.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7022..7441)
                     /gene="trxC"
                     /locus_tag="ACIYSB_003327"
     CDS             complement(7022..7441)
                     /gene="trxC"
                     /locus_tag="ACIYSB_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGC5430309.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7648..8685
                     /gene="yfiF"
                     /locus_tag="ACIYSB_003328"
     CDS             7648..8685
                     /gene="yfiF"
                     /locus_tag="ACIYSB_003328"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGC5430310.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8733..9422)
                     /gene="ung"
                     /locus_tag="ACIYSB_003329"
     CDS             complement(8733..9422)
                     /gene="ung"
                     /locus_tag="ACIYSB_003329"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGC5430311.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9727..10110
                     /gene="grcA"
                     /locus_tag="ACIYSB_003330"
     CDS             9727..10110
                     /gene="grcA"
                     /locus_tag="ACIYSB_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGC5430312.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10166..10753)
                     /gene="eamB"
                     /locus_tag="ACIYSB_003331"
     CDS             complement(10166..10753)
                     /gene="eamB"
                     /locus_tag="ACIYSB_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGC5430313.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10856..11737
                     /gene="yfiE"
                     /locus_tag="ACIYSB_003332"
     CDS             10856..11737
                     /gene="yfiE"
                     /locus_tag="ACIYSB_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5430314.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11946..13280)
                     /gene="srmB"
                     /locus_tag="ACIYSB_003333"
     CDS             complement(11946..13280)
                     /gene="srmB"
                     /locus_tag="ACIYSB_003333"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGC5430315.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13412..14149
                     /gene="trmN"
                     /locus_tag="ACIYSB_003334"
     CDS             13412..14149
                     /gene="trmN"
                     /locus_tag="ACIYSB_003334"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGC5430316.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14134..15756)
                     /gene="nadB"
                     /locus_tag="ACIYSB_003335"
     CDS             complement(14134..15756)
                     /gene="nadB"
                     /locus_tag="ACIYSB_003335"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGC5430317.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            16012..16167
                     /gene="rseD"
                     /locus_tag="ACIYSB_003336"
     CDS             16012..16167
                     /gene="rseD"
                     /locus_tag="ACIYSB_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGC5430318.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16164..16739
                     /gene="rpoE"
                     /locus_tag="ACIYSB_003337"
     CDS             16164..16739
                     /gene="rpoE"
                     /locus_tag="ACIYSB_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGC5430319.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16772..17422
                     /gene="rseA"
                     /locus_tag="ACIYSB_003338"
     CDS             16772..17422
                     /gene="rseA"
                     /locus_tag="ACIYSB_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGC5430320.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17422..18378
                     /gene="rseB"
                     /locus_tag="ACIYSB_003339"
     CDS             17422..18378
                     /gene="rseB"
                     /locus_tag="ACIYSB_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGC5430321.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18375..18854
                     /gene="rseC"
                     /locus_tag="ACIYSB_003340"
     CDS             18375..18854
                     /gene="rseC"
                     /locus_tag="ACIYSB_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGC5430322.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            19052..20851
                     /gene="lepA"
                     /locus_tag="ACIYSB_003341"
     CDS             19052..20851
                     /gene="lepA"
                     /locus_tag="ACIYSB_003341"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGC5430323.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20867..21841
                     /gene="lepB"
                     /locus_tag="ACIYSB_003342"
     CDS             20867..21841
                     /gene="lepB"
                     /locus_tag="ACIYSB_003342"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGC5430324.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            22113..22793
                     /gene="rnc"
                     /locus_tag="ACIYSB_003343"
     CDS             22113..22793
                     /gene="rnc"
                     /locus_tag="ACIYSB_003343"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGC5430325.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22790..23695
                     /gene="era"
                     /locus_tag="ACIYSB_003344"
     CDS             22790..23695
                     /gene="era"
                     /locus_tag="ACIYSB_003344"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGC5430326.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23707..24435
                     /gene="recO"
                     /locus_tag="ACIYSB_003345"
     CDS             23707..24435
                     /gene="recO"
                     /locus_tag="ACIYSB_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGC5430327.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24447..25178
                     /gene="pdxJ"
                     /locus_tag="ACIYSB_003346"
     CDS             24447..25178
                     /gene="pdxJ"
                     /locus_tag="ACIYSB_003346"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGC5430328.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25178..25558
                     /gene="acpS"
                     /locus_tag="ACIYSB_003347"
     CDS             25178..25558
                     /gene="acpS"
                     /locus_tag="ACIYSB_003347"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGC5430329.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            25979..26059
                     /gene="shoB"
                     /locus_tag="ACIYSB_003348"
     CDS             25979..26059
                     /gene="shoB"
                     /locus_tag="ACIYSB_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGC5430330.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         6403 a         6522 c         6902 g         6301 t
ORIGIN      
        1 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
       61 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat tatgcgaaag
      121 gccatcctga cggatggcct ttttgcattg gcgcagaaaa aaatgcctga tgcgacgctg
      181 cgcgtcttat actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc
      241 cacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa taaccatttt
      301 cctgctaact aaattcatgg ttaaggttgc ataatgatat gcaacaaatg tataacattt
      361 tatttaccaa aaaaaaataa acaaaagcga ccgacaaaag catcggatta cggcaggaga
      421 cataatggca tggctgaaag tactgtaacg gcagacagca aactgacaag tagtgatact
      481 cgtcgccgca tttgggcgat tgtgggggcc tcttcaggta atctggtcga gtggttcgat
      541 ttctatgtct actcgttctg ttcactctac tttgcccaca tcttcttccc ttccgggaac
      601 acgacgactc aactactaca aacagcaggt gtttttgctg cgggattcct gatgcgccca
      661 ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa atcgatgctg
      721 ttatcggtgt gtatgatgtg tttcggatcg ctggttatcg cctgcctccc aggttatgaa
      781 actataggta cgtgggctcc ggcattattg cttctcgctc gtttatttca gggattatct
      841 gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt tgaagggcgc
      901 aaaggttttt acgcatcatt tcagtatgtg acgttgatcg gcggacaact gctagcccta
      961 ctggttgtcg tggttttaca acacaccatg gaagacgctg cactcagaga gtggggatgg
     1021 cgtattcctt tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt acgtcgtcag
     1081 ttagatgaaa cttcgcaaca agaaacgcgc gctttaaaag aagctggatc tctgaaagga
     1141 ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc gggctccctt
     1201 tgtttctata ccttcactac ttatatgcag aagtatctgg taaatactgc gggaatgcat
     1261 gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct tattcaacca
     1321 ctcattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg tttcggttcg
     1381 ctggcagcca tttttaccgt tcctattctc tcagcattgc aaaacgtttc ctcgccttat
     1441 gccgcttttg gtctggtgat gtgtgccctg ctgatagtga gtttttatac atcaatcagt
     1501 ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt tggtctgtca
     1561 tatgcggtcg ctaatgctat atttggtggt tcggcggagt acgtagcgtt gtcgctgaaa
     1621 tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt ggtggcgttt
     1681 ctggtttctt tgatgctaca tcgcaaaggg aaggggatgc gtctttagtg acgggtcagt
     1741 tgccagacgg tatagccggt gcttgcaccg gcgacatccc aggccaaatc cttccagctc
     1801 cagccgctcc cttcggggcg gctatcccaa agctcttttg acgcccccaa actgacagag
     1861 aacatcaatc caaacatggc actgcgatcc cggctcatcc cctgatgctg tgaatattca
     1921 tttccggcgg cggaaagcat cgccgaggcg ataaagtgtt gagctttatc ctgcccgctc
     1981 cagctatcgt tagccatatg gctgcatcca gaaagtaaca atagcgaaca gacaaaaaga
     2041 atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg tgattacagg
     2101 atgcggctaa ttaatcggtc gatgcggata cggcgcaaac ggcggatgag tttacgaacc
     2161 ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat ggtggtatgc
     2221 tcgcggatca gctccagttc tttctctcgc tgtggcgcca gctcaagttg cggatcgtgg
     2281 atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt attaccggtg
     2341 atcaacatcc acttatcatc aacccacatc cctttcaggt gataggtgtt gtcgtcatct
     2401 ttccataacc gaaccactag ctggtcagta ttgacgtaat actgcaaacg gctcaggaaa
     2461 cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa aggttcatct
     2521 tccggaatgt agaagtcatt cgcggtttta tcaccaacaa taatttcgac ctttttccct
     2581 tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa gtatggcgta
     2641 cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatggtctt gttcaacaga
     2701 ctcgatttcc ccagccccac tagcggcgtt acagaaagct gatcgttgtc ggcatcgccc
     2761 tggaaatgat aagcggcatc acgcagctcc tggcggaaca gacgaatatc gttcttgatt
     2821 tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc attcataata
     2881 ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat cagatgataa
     2941 cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct ggcaccgcta
     3001 taaagtacgc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag ggcttcacga
     3061 gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg cgccatgcgg
     3121 cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg ttgtgcacga
     3181 tgccagtcga ccagcacccg cacatccagt tccggacgct gccttttagc ctcatacaac
     3241 gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcgac aatgcaaatg
     3301 cgctgcttcg cgctggctat tttttccagc agcgtctccc ggaagtcggc gggagcgtaa
     3361 aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag gtgttgttga
     3421 tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt tcattgaagg
     3481 gtcctctgtg caatgcagac gacataagcg ggcaataata acaccagtcc cgattaagtg
     3541 gtcaacattt ccagtacctt actcatgatt cctcgcgctg ggcaagattt agcgtaagcc
     3601 caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg gctagcgcca
     3661 ccatgccacg attgtttggc atcgtaatac cattcagacg ttgtagtccg tgatctcgcg
     3721 tataggtaat caacttttcc attaagcgtc gacctaagcc taaccctttg agatccgagc
     3781 gaaccagtac agcaaattcg gcatcgatgt tatcaggatc ggaaatcgca cgcgtgacgc
     3841 cgaggatctc ttccgtttga tcaatacgtc gtaccgctac aaacgccatt tcccgatcgt
     3901 agtcgatctg tgtcatgttg gctaaatctt catgggtaaa ttcgttgatc tcgctaaagt
     3961 agcggtaata aagatcttct ttggtgactc gcgaaatgaa ttgctgaagt tgtggctcat
     4021 cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct acccattctt
     4081 ccagctgatg cggataaggg cgcactgcca gccgactctc gttatcgcct tcaaacggcg
     4141 agatatccag cgtgacatcc agcgcggtaa attcactgcc agaagccagc aaaggatgaa
     4201 tatccagacg ctgaatttcc gggcaatcga caatcaagtt ggaaacctgc accagaagct
     4261 ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg cgcacgaatc tttttacttt
     4321 tgatcccctg aataaccaga tagcgggcca ggttcatgtt cagcggcggc agtgcgacga
     4381 cggcttgatc ttcaggacgc cactccacac cgccttcacc cagcatgatc aacggcccga
     4441 aaaccggatc gtgctcaacc acaacccgca actcctgagc gccagcacgg ttagccatac
     4501 tttgcaccaa caggccgtgg acccgcgcct gtggccaggc catttttacg cgatcgaaaa
     4561 tagcgttcgc cgcttgctgg acttcattgg ctgtacgcag gtaaagcatg acgccctgaa
     4621 cttccgattt atgtggaata tccggcgaac gcaatttcag cgccaccgga taaccaatct
     4681 gttcggcaat atgcaccgct tcggtgctat cgctggcaat ccaggtaggg agcgtgttca
     4741 tgccatacgc ttgcaggatg ggctgaactt catgggtatc gagcgacgta gccccttcgg
     4801 caatcgcctg ttgcaacaga agatgcgctt ctgcggtatt ggaagtcaga ttgctgggca
     4861 acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctccaccata tgcataaaag
     4921 cagtgatggt tccttccggg gtacggtagg tcggcagccc ggcttcgctg aataaacgtc
     4981 gtgcctcttg cgaggagtgc tcgccgcacc agttcgtcag caaagagaca tatttgctgc
     5041 ggggatgatg ctttaccgct tcaattaata cttgcgcgct ttctgttgcg ggagcagcgg
     5101 cgctgggcga atgaataacc atcagcgcgt caaaatcctg gctgtggagc agaatatcca
     5161 gcgttttaat atagtgctca ctgctggcgt catcgcgtag atcgagcggg ttagatattg
     5221 ccacatgttc tggcagtgca tcgcgcagtt tctggcaggt ttcttcgctt agcgttgcca
     5281 gcttgccatt gcgtgaccat aaggcatcca gcgccagcgc ggcaggcgca gcaccgttgc
     5341 tgataatcat cagccggtcg ccacgtagcg ggcgcatatg gctaagggtt tccaccgccg
     5401 aaaacagctc gtgggtgtcc tgtacccgca acaaaccggc acgctgaata gccgcatccc
     5461 atgccgggtc cattcctgcc gtcgtgttga gcagtcgctg tgccgccggg ctacgtccgc
     5521 ttttaatcac cagaatcggt ttattacgcg aggcactacg ggccgccgaa acaaagcgtc
     5581 gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg tcgcgtgcca
     5641 gatagtcaag caattcatca acgtcgatat ccaggctgtc gccgagcgca ataaagtagg
     5701 aaaagcccat cttacgctgt tgcgcccagt cgaggatggt gttggagacg gcagccgatt
     5761 gcgaaataaa cgccagcttg ccgcgtttaa tcggcacagg cgaaaagctg gcattcagac
     5821 cttgccaggg agccagtaaa cccagactgt ttggtccaag caggcgcatg ttatggcgca
     5881 gggcgcaggc gcggagatct tcgtgttgcg atgccggggc ggaaagaata atgcaggttt
     5941 tacagccttt ctcgccgagc tcttccagaa gagcaagatt acggctggca ttggtacata
     6001 aaaccgcaag gtcgggtgta aagggcaagc tggcaatatc cggccaggcc aacacaccca
     6061 acaccgcttt ccaggctggc gtcaccggga gtaccggtcc gttaaagcct cccgccagca
     6121 ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgaggcgcca attaccgcta
     6181 tcgattttgg tcgcagtagt gcttccagtc ctcgctgact cataccggtc tcccgcagaa
     6241 gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgctacc cagatgttgc
     6301 gtttttcctg ccagatagcg tgttttaaag cgggtaaaat gctcgcctaa ccctgccgcc
     6361 gccccggtat cgccggccat atctaacagt gcgatggcta cctcggcagt acaatattgg
     6421 ccttcagcct gggcttcacg caggcgatag gcagaaagcc gggaaagatc gacggaaatg
     6481 acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc cggccaggta
     6541 ccatcgagca tgataaacag cggtggctta ccggcaggtg gtgtgaagat cacttcccgt
     6601 tgctcatcag catacgaagc gggaaagacc accattggct gatagtccgg gttttgcacc
     6661 aactccagca aatcctgcga gggttcggta cgcgaccatt gaaacgcaac ggtatcaggc
     6721 aaaatatcag caatgagacg cccggtatta ctgggcttca ttggctcggt gtcgaacatc
     6781 agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag acataatttc
     6841 tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag aaaaggacgt
     6901 gttgcgcgcg caatacgctc ggcgcgtaac tggagaacag cgttttcggt cataagagag
     6961 cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg ccccggtaag
     7021 attaaagaga ttcgttcagc cagctatcga acggcgcttt cggtactgcg ccattaagca
     7081 tgtcgacaac ctgaccgttt ttgaaaatca tgatcgtcgg tatactacga attccaaagc
     7141 gactgctcaa ttcacgttca gcttcggtat tcactttcac aaagcgcact ttaccgctac
     7201 gctcttgcgc gacatcttca aaaattggtg cgaaattacg gcaggggccg caccacggtg
     7261 cccagaagtc gatcaccaca ggtagatcat ccttcagcaa tttgtcgagt gtttcaccgg
     7321 tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat tttgccgcat
     7381 cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa acggtattca
     7441 taactaacct cgggatgagt aaggagattt atcggcaacg acgccggtat gtttctaata
     7501 tgttacatat tatcggggac attgttccag acaacaatct gttttattca ataggtatga
     7561 tagtcgcagg ctttggtacg aaatcatggc gaaccgcgcg gacttcgggt aatctgcgcg
     7621 cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa aagcggcaag
     7681 gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca caacccgcgt
     7741 accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg tcgccccgcc
     7801 cgcgatgaca aacagagtca gccccgtgac cgcaagtggg aagattcgcc gtggcgcacg
     7861 gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg catcagtggt
     7921 aaaagtttta ttgatccgga agtgttgcgt cgtcagcgtg cggaagaaac ccgcgtctac
     7981 ggcgaaaatg catgtcaggc acttttccag agccgtccgg aagcgattgt tcgcgcctgg
     8041 tttatccaga gtgtaacgcc gcgttttaaa gaagctttgc gctggatggc agcaaaccgc
     8101 aaagcgtacc atgtggtgga tgaagcggaa ttgacaaaag cgtcaggcac ggaacatcac
     8161 ggaggcgttt gcttcttgat caaaaagcgt aacggtacaa ccgtgcagca gtgggtaagc
     8221 caggcaggcg cgcaggattg cgttctggca ctggaaaacg aatctaaccc gcataacctg
     8281 ggtggcatga tgcgcagctg cgcgcacttc ggcgtgaaag gtgtcgtggt gcaggatgcg
     8341 gcactgctgg agtcgggggc ggctatccgt accgcagaag gcggcgcaga gcacgttcag
     8401 ccgattactg gcgacaacat tgttaacgtg ctggatgatt tccgtcaggc aggttacacc
     8461 gtagtgacca cttccagcga gcagggtaaa ccgctgttca aaactagtct gccagcgaaa
     8521 atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg cgatccgaac
     8581 gatctgcgcg tgaagattga cggtacgggc aacgttgccg ggctgaatat ctctgtcgca
     8641 accggcgttt tgcttggtga atggtggcgt cagaataaag cctgataaat cagccgggtg
     8701 gcaactctgc catccggcat ttccccgcaa atttactcac tctctgccgg taatactggc
     8761 atccagtcaa tcggcgtctc gccacgttgt tccagccact gatttgccag cacaaaatgg
     8821 ttgcagccaa agaatccacg atgcgccgaa agcggcgacg gatgcggtgc tttcagtaca
     8881 tgatggcgtt gcttatctat aatcgcccct ttcttttgcg catgcgatcc ccacaacaaa
     8941 aacaccacgc cttcgcgatg ctggttaatc aggctgatca ctttatcggt gaaggtttcc
     9001 cagccgaggc tggcgtggga atgcgcctga cctgcgcgta ccgtcaacac agtattgagt
     9061 agcagaacgc cctgacgcgc ccagctttca agataaccat gattagggcg ggtgaagccc
     9121 ggaatagtat tttccagctc tttatacata ttcaataatg acggaggaat ggcaatgccg
     9181 ggacgaacgg aaaatgccag accatgcgcc tgtcccggtc cgtgataagg atcctggccg
     9241 agaatcacca ctttaacgtc acccaactct gtaaagcgga acgcgttaaa gacatctttt
     9301 tgtggtgggt agatagtgac gccggactgc cgctcgctgg cgacggtctg aagggtatta
     9361 agaaaatagg gttgctgctt ctcttcagcc agcacgtcat gccaggttaa ttcgttagcc
     9421 atctcgctct cctgcgaatc ttcaatccgc ctagcttaac tgcttctttc tacagaacaa
     9481 aatcatgctc attttttaga cttaaacgag tgcaaaaaaa tgtaaattgg taaaatttca
     9541 cacccgcata agttgatgta aaacaataaa atccatttgc gaccatactt gatgtgtggt
     9601 ttttattgat ttaaatcaaa gattcaaggg tgtttgagga gtatatatac actcaagcaa
     9661 caatggtttt accaattggc cgcgacaggc tgaacaaatc aaataatttt gccggggagg
     9721 catcacatga ttacaggtat ccagattact aaagccgcta acgacgatct gctgaactct
     9781 ttctggctgc tggacagcga aaaaggcgaa gcgcgttgca tcgttgcaaa agcaggttat
     9841 gcagaagatg aagtggttgc agtaagcaaa ctgggtgaca ttgaataccg tgaagttcca
     9901 gtagaagtga aaccagaagt tcgcgttgaa ggtggtcaac acctgaacgt taacgttctg
     9961 cgtcgcgaaa ctctggaaga tgcagttaag catccggaaa aatatccgca gctgaccatc
    10021 cgtgtatccg gttatgcagt tcgctttaac tctctgactc cggaacagca gcgcgacgtt
    10081 atcgctcgta cctttactga aagcctgtaa ggcttaagta gcaagttact aagcggaaga
    10141 gggcgacctc ttccgctttt tttcgttaat agaaaatgcg taccgcgcaa tagaccagca
    10201 acagggcaag cacgatattt aactggcgac catactggcg aaacaatcgc tgaaacagat
    10261 gccccgccag cgcccagcac acattgccaa acgtcccaat catcgccagc aaaacgctga
    10321 cgccaactac ccagcttaac gcctgtgttt gcggcagaac aaacgtcgac agtgccgtaa
    10381 caccgtacaa aatgattttg acgttcacaa actgcaaagc aaagctggcc caaaagctga
    10441 ttggttttgc ctgaagtccg tcttcctttg ttgggctggt ggcgattttc cacgccagcc
    10501 agacaatata tgccgccccc gcccaactca aaaggtgtac cgctgccggg tcaatcactg
    10561 ccagtgaaaa tgaaatgccc gcacacagta acatcacaat caaaaatccc agactcatcc
    10621 ctgccagcac gcgggtactt tgacgaaatc catgcgacgt agcagagcta agggcgagaa
    10681 tattgttcgg tcctggcgtc atagcggtaa tcagggtgta agtccaaaaa gcacttaaaa
    10741 gggtcggtgt cacttgttat ctcctctgtt ttggtagaca gtacccgaca cggaacattt
    10801 aaaaaaatga atgtttttat attctagatt cgatattttc gataggtttg gggttatgga
    10861 tctgcgccgt tttattacgc ttaaaaccgt ggtggaagag ggttcctttt tgcgagcttc
    10921 gcaaaaattg tgctgtacac aatcgacggt gacttttcat attcagcagc ttgagcagga
    10981 gttttcagtc cagttatttg agaaaattgg ccgacggatg tgccttaccc gcgaaggaaa
    11041 aaagttgctg ccgcacattt atgaacttac ccgagtgatg gatacgcttc gtgaggctgc
    11101 caaaaaggag tcagatccgg acggggaact acgcgttgtt tcgggcgaaa cgttgctctc
    11161 ttaccgaatg ccgcaggtgt tgcagcgttt tcggcaacgc gccccaaaag tgcgtttatc
    11221 gttgcagtcg ctgaactgtt acgtgatccg tgatgcgcta ctgaatgatg aggccgatgt
    11281 tggcgtcttt tatcgtgtag ggaatgatga tgctctgaat cgacgagagt tgggtgaaca
    11341 atcactggtg ctggtggctt caccgcaaat tgcagatgtc gattttactg agccgggaag
    11401 acataacgcc tgtagcttta ttatcaacga accgcaatgt gtcttccggc agatatttga
    11461 gagcacgctg cgtcagcggc ggatcacggt ggaaaacacc attgagctta taagtatcga
    11521 aagcatcaag cgttgtgtcg cggcgaatat cggggtcagc tacctaccgc gctttgcggt
    11581 ggcaaaagag ttggagtgcg gagaattaat tgaattgcct tttggcgaac agtcgcagac
    11641 cattacggcc atgtgcgcac atcatgctgg aaaagcggtt agcccggcga tgcacacttt
    11701 tattcagtgt gttgaagaga gttttgtggc tggataaaaa taatgtcggg attaaacccg
    11761 gcatttaact tgtcgacaaa gtgcgcttta ttcatgccgg atgcggcgtg aacgccttat
    11821 ccggcctaca agatcgtgca aattcaatat attgcatgag ccacgtaggc ctgataagcg
    11881 tagcgcatca ggcagttttg cgtttgccag cacttttaat accaggctta aacctggcat
    11941 tgagattact cttctgtcgt ttgcggtggc acgcccgttc cgcttggttt acggcgctta
    12001 ccaatatttt tggtgtcgcg atggcgtttt ttcacccgcg gcttctcttt ctctttagct
    12061 tttttcttct cagcacgttt agccagtact ttcttcgatg gcttgccggt ctgcttttcg
    12121 cttggcgcac gcgttttcgg gcgtaactca tcaataacgc gagctttaat tggctcttca
    12181 atatagcggc ctactttacc cagcagcaga tggtcatggg cttccaccag cgaaattgcg
    12241 gtgcctttac gaccggcgcg cgcggtacgt ccgatacggt gcaaataagt atcgccactg
    12301 cgcggcatat cgaagttaaa gacgtggctg acgtcaggaa tgtcgatacc gcgcgcggca
    12361 acatcggttg cgaccagtac gtttacgcga ccttcggtca aacgcttgat cgcttcgtta
    12421 cgcttgccct gtaccatctc accttcgaga tagcagttgt tgatgcccgc ttcgcgcagc
    12481 cagtttgcca gctcatgcac acgctcacgc ttacgcacaa acacaattga gcgggtcgct
    12541 tccggctgtt ttaacagatg caccagcaac gcggttttat gctcaagatc atcggcgcgg
    12601 taataccact gatgaatttt tttgcgctca cgggtggagg gattggcaga aacttccacc
    12661 ggatcttcca gcagacgctc ggcaaagtcc tgaatcgcat cgccttccag cgttgccgaa
    12721 aagagcaggg tctgtttacg ccagcgcgtt tcgccagcaa tatgttcgat atcctgagcg
    12781 aagcccatat ccagcatacg gtctgcttcg tcgaggatca gcgtttcaac cgcgcggcaa
    12841 tcgaagttct cttcttttat gtattgcagc agacgtccgg tcgtggcgac cacgatgtcc
    12901 tgattttcgc tgaacacttc cgcgtggttc atataggcta cgccgccggt gatggtggcg
    12961 atatccagat gcgtatgttt cgccagttcg cgggcatgat cggccacctg catcgccagc
    13021 tcgcgagttg gggtgaggat caaaatacgc ggcggaccgg atttcttacg cgggaaatcg
    13081 agcaggtgct gcaacgctgg cagcagatac gccgccgttt taccggtgcc tgtcggcgca
    13141 gaaccgagta catcacggcc atcgagcgca ggcggaatgg cggcagcctg aatggcggtc
    13201 gggcgagtga aacctttatc ctggagggct tccagcaggc tttcgtcgag ttcaagttcg
    13261 gaaaaagtcg ttacagtcat gttctacctc tgtgtggggc gctgattata gacgttacgg
    13321 ctgcaatctt catctgtttg tatggatatc gcttctcggg tattgttctc acccggtgct
    13381 atcgccgttt ttcctgcaag gttttatttt catgtcacag tctacatccg tgcttcgtcg
    13441 taatggattt acttttaaac agttttttgt tgctcacgat cgctgtgcga tgaaagtggg
    13501 aacggatggt attttattgg gcgcatgggc accggtggct ggggtaaaac gttgccttga
    13561 tatcggcgcg ggtagcgggt tgctggcatt aatgctggcg cagcgaaccg atgacagcgt
    13621 gatgattgat gcagttgaac tggaaagtga agctgccgcg caggcgcagg aaaatatcaa
    13681 ccagtccccg tgggcagagc ggattaacgt ccatacggcg gatattcagc agtggatcac
    13741 acagcagaca gtacgcttcg atttaatcat cagtaaccca ccttactatc agcagggagt
    13801 agagtgctcg acacctcaac gggaacaggc tcgctatacc actacgcttg atcacccatc
    13861 gttgctgacc tgtgcggcgg agtgcattac cgaagaggga tttttctgcg tagtgctgcc
    13921 agagcagatt ggtaatggtt ttacggagct ggcattaagc atgggctggc atttacgttt
    13981 gcggacggat gtggcggaaa acgaagcgcg actgccgcat cgggtgctgc tggcattctc
    14041 cccgcaggcg ggagaatgct ttagcgatcg cttagtcatt cgtgggccag accagaacta
    14101 ttccgaagcg tatacggcgc tgacccaggc tttttatctg tttatgtaat gattgccggg
    14161 ggaaaggatc gacggaccgg aatgggtgag cagttccgga taatccagcg tgaaatgcaa
    14221 cccccgactc tctttacgca tcattgcaca gcgaacaatc aactcggcaa cctgtaccag
    14281 attacgcagc tccagcaaat tatttgagac gcggaaatgg gcgtaatatt cgtctatttc
    14341 ttgttggagc atggttatcc gccgcagggc gcgttccagg cgcttcgttg tgcgcacaat
    14401 gccaacgtaa tcccacataa acagacgtag ctcgtgccag ttatgctgaa ttactacccg
    14461 ttcgtcaggg ttctcaacgc ggctttcatc ccacggcggt aacgtactga tgtcgtgggc
    14521 ataaggcata cgtctggtga tatcttccgc cgccgaccag ccatagacca gacactccag
    14581 caatgaattc gaggccatgc ggttagcgcc gtgtaagccg gtataactca cctcgccaat
    14641 ggcatacaag ccctcgacgt ccgtacgccc atgatcatca accattacac caccgcaggt
    14701 ataatgtgca gcaggcacaa tcggtaccgg ttcttgtgtg agatcaatcc ccagcccgag
    14761 cagcttttca taaatcatcg ggaaatgctg gcgaataaaa tcggcgggct tatggctgat
    14821 atcaaggaac atacaatctg cgccgaggcg tttcatttca tggtcaatgg cgcgggcgac
    14881 aatatcgcgc ggggccagtt cgccgcgctc atcaaaatcg ggcataaaac gcgtaccatc
    14941 cgggcgcttg agataagcgc cttcgccgcg cagtgcttct gttaacagga aattgcgtgc
    15001 ctgtgggtga tatagcgcgg tagggtggaa ctgattaaat tcgagattgg caacccggca
    15061 gcctgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
    15121 ctgataaacc ttcgacgcac cgccggttgc cagcaccacc gcttttgcgt ggcaggtttc
    15181 caccgtttct ttattacggt tccataccca ggcgccaaca acccgtcgcg tgcccggcag
    15241 gccaattttg tcagaaacaa tcagatcaac cgcgttgctg cgctccagca cgcgaatatt
    15301 cggatggttc agcgccttgc tcaccagcgt ggtttctact tctctaccgg tggcgtcggc
    15361 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt aactttcttc
    15421 gccattcggt tgaatgtggg tatcaaacaa caccccctgg tcgattagcc attgcacaca
    15481 ggatcgtgca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
    15541 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
    15601 accgccttgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
    15661 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccgataat
    15721 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
    15781 tggtttggtc agcatagcat catgttgtgc ggataaacac ctgctatttt aatatttgtt
    15841 acagttgcta aacacgctga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
    15901 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
    15961 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
    16021 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
    16081 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
    16141 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
    16201 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
    16261 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
    16321 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
    16381 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
    16441 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
    16501 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
    16561 gaactattga gtccctcccg gaagatttac gcatggcaat aaccttgcgg gagctggatg
    16621 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
    16681 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
    16741 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
    16801 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
    16861 gaaaacctgg gaaagctatc acttaatccg tgactcaatg cggggtgata ctcccgaggt
    16921 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
    16981 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaga aaatgccatt
    17041 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag ccgcatgcgt
    17101 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
    17161 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
    17221 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
    17281 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
    17341 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gaattcagac
    17401 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
    17461 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
    17521 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
    17581 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
    17641 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
    17701 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
    17761 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
    17821 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
    17881 tacagctaca tcgtgtggat ggacaccgaa tcgaaattac cgatgcgggt tgatctcctt
    17941 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattg cttttaacgt caatcaggat
    18001 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
    18061 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
    18121 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
    18181 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
    18241 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
    18301 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
    18361 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
    18421 cgctggtcag ttgtgatgtt aaagcttcat gcagcagctg cgcttcacgc gccggttgcg
    18481 gtagccgcgt gttaaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
    18541 atgagccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
    18601 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
    18661 ttcagctact ctttgcttct gacgtcgcag cattatgtgg tgcgatcctc ggtggtattg
    18721 gcgggttcct gattgcgcgc ggctactcgc gtaagtttgc tgcccgggca gaatggcaac
    18781 cgatcatctt aagcgtggca ttgccgcctg gccttgtgcg atttgaaaca tcttcggaag
    18841 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
    18901 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
    18961 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
    19021 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
    19081 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
    19141 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
    19201 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
    19261 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
    19321 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtagaagc
    19381 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagttg tgccggtact
    19441 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
    19501 cgtcggcatc gacgccaccg acgcggtgcg ctgttcagcg aaaaccggcg ttggtgtgca
    19561 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
    19621 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
    19681 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
    19741 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
    19801 gaaatgtggc gaagtaggct ggctcgtatg tgcgattaaa gatatccacg gcgctccagt
    19861 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
    19921 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
    19981 ccgtgacgcg ctgggtaaac tcagcctgaa cgatgcctca ctgttctatg agccggaaag
    20041 ctccagcgcg ctgggctttg gtttccgctg cggcttcctc ggcctgctgc acatggagat
    20101 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
    20161 agtgtatgaa gttgaaacca cgtcaagaga agttatctac gtcgacagcc catccaagct
    20221 gcctgcggta aataacatct acgaactgcg cgagccgatt gcagagtgtc acatgctgct
    20281 gccgcaggca tatctcggca acgttattac gttgtgcgta gaaaaacgcg gcgtgcagac
    20341 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
    20401 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
    20461 caacttcaag cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
    20521 acgtgttgat gcgctggcgt tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
    20581 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
    20641 agcgattggt acgcacatca ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
    20701 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
    20761 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
    20821 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
    20881 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
    20941 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggggactc
    21001 actggataaa gcaacgttga aaaaggttgc gccgaagcct ggctggctgg aaaccggtgc
    21061 ttctgttttt ccggtactgg ctatcgtatt gattgtgcgt tcgtttattt atgaaccgtt
    21121 ccagatcccg tcaggttcga tgatgccgac tctgttaatt ggtgatttta ttctggtaga
    21181 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
    21241 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
    21301 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
    21361 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
    21421 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
    21481 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
    21541 cgagcgtaaa gagacactgg gtgatgtgac gcaccgcatt ctgacagtgc cgattgcgca
    21601 ggatcaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
    21661 tccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
    21721 gggctttgtg ccggaagcga atctggtcgg tcgggcaacg gctatctgga tgagcttcga
    21781 taagcaagaa ggcgaatggc cgactggtct gcgcttaagt cgcattggcg gtatccatta
    21841 atagccatct tcgttcacgt tgtcgccgtt atggcgacaa cgtgaattat ttatgagata
    21901 aatctcccgt ggctaacgac atcccccgtc gttgtgtata gaatattccc ccgaagttta
    21961 aggttggcac ctccaggttg ccacggcaca cgaaacagcg ttggtcccca tataccggta
    22021 aactgaaact gcagcgaagc agttagcaga accatgtata tcaggtctgt ttcgtgtgct
    22081 gaattgttga cgcatttatt tattggtatc gcatgaaccc catcgtaatt aatcggcttc
    22141 aacggaagct gggctacact tttaatcatc aggaactgtt gcagcaggca ttaactcatc
    22201 gtagtgccag cagtaaacat aacgagcgtt tagaattttt aggcgactct attctgagct
    22261 acgttatcgc caatgcgctt tatcaccgtt tccctcgtgt ggatgaaggc gatatgagcc
    22321 ggatgcgcgc cacgctggtc cgtggcaata cgctggcgga actggcgcgc gaatttgagt
    22381 taggcgagtg cttacgttta gggccaggtg aacttaaaag cggtggattt cgtcgtgagt
    22441 caattctcgc cgacaccgtc gaagcattaa ttggtggcgt attcctcgac agtgatattc
    22501 aaaccgtcga gaaattaatc ctcaactggt atcaaactcg tttggacgaa attagcccag
    22561 gcgataaaca aaaagatccg aaaacgcgct tgcaagaata tttgcagggt cgccatctgc
    22621 cgctgccgac ttatctggta gtccaggtac gtggcgaagc gcacgatcag gaatttacta
    22681 tccactgcca ggtcagcggc ctgagtgaac cggtggttgg cacaggttca agccgtcgta
    22741 aggctgagca ggctgccgcc gaacaggcgt tgaaaaaact ggagctggaa tgagcatcga
    22801 taaaagttac tgcggattta ttgccatcgt cggacgtccg aacgttggca aatccacatt
    22861 gttgaacaaa ctgctggggc agaaaatctc catcacttcc cgcaaggcgc agacaactcg
    22921 tcaccgcatt gtggggatcc atactgaagg cgcgtatcag gcgatctacg tcgatacacc
    22981 gggcctgcat atggaagaaa aacgcgccat taaccgcctg atgaacaaag cggcgagcag
    23041 ctctattggc gatgttgagc tggtgatttt tgtcgttgaa ggcacccgct ggacgccgga
    23101 cgacgaaatg gtgctcaaca aactgcgcga aggcaaagcg ccggtaatcc tcgcggtgaa
    23161 caaagtggac aacgtgcagg agaaagccga tctgctgccg cacctgcagt tcctggcaag
    23221 ccagatgaac ttcctcgata tcgtgccaat ctctgccgaa accgggctga atgttgacac
    23281 tattgcggca atcgtgcgta agcatctacc tgaagcgact catcacttcc cggaagatta
    23341 catcaccgat cgctcacagc gttttatggc gtctgaaatc atccgcgaaa aactgatgcg
    23401 tttcctcggc gctgaactgc cgtactccgt gaccgtggag atcgaacgtt tcgtctctaa
    23461 cgaacgcggt ggttatgaca tcaacggttt gattctcgtt gagcgtgaag ggcagaagaa
    23521 gatggtcatt ggcaacaaag gggccaagat caaaaccatc gggattgaag cgcgtaaaga
    23581 catgcaggaa atgttcgaag cgcctgttca ccttgaactg tgggtaaaag tgaaatccgg
    23641 ttgggccgac gacgaacgcg cactgcgcag tctcggttac gttgacgatc tttaagagta
    23701 actccgatgg aaggctggca gcgcgcattt gtcctgcata gtcgcccgtg gagcgaaacc
    23761 agcctgatgc tggacgtctt cacggaggaa tcggggcgcg tgcgtctggt tgccaaaggc
    23821 gcacgctcta aacgctctac cctgaaaggt gcattacagc ctttcacccc tctcttgcta
    23881 cgttttggcg ggcgtggcga agtcaaaacg ctgcgcagtg ctgaagccgt ctcgctggcg
    23941 ctgccattaa gcggtatcac gctttacagc ggtctgtaca tcaacgaact tctctcccgc
    24001 gtactggaat acgagacgcg cttctctgaa ctttttttcg attacttgca ctgcattcag
    24061 tctcttgcag gggtcactgg tacgccagaa cccgcgctgc gccgctttga actggcactg
    24121 ctcgggcatc tgggttatgg cgtcaatttt acccattgtg cgggtagcgg cgagccggta
    24181 gatgacacca tgacgtatcg ttatcgcgaa gaaaaagggt ttatcgcaag cgtcgttatc
    24241 gacaataaaa cgttcaccgg aaggcagtta aaagcgttaa acgcacggga atttcctgac
    24301 gcagacacac tgcgcgccgc gaaacgcttt acccgcatgg cgcttaagcc gtatcttgga
    24361 ggtaaacctt taaagagcag ggaactgttc cggcagttta tgcctaagcg aacggtgaaa
    24421 acacattatg aatgatgagg attgtcatgg ctgaattact gttaggcgtc aacattgacc
    24481 atatcgctac gctgcgcaac gcgcgcggta ccgcttaccc ggatccggtg caggccgcgt
    24541 ttattgccga gcaggcggga gcggacggca ttaccgtgca tttacgtgaa gatcgccgtc
    24601 acattactga ccgcgacgtg cgcatcctgc gtcagacgct ggatacccgc atgaatctgg
    24661 agatggcggt gaccgaagag atgctggcga tcgccgttga gacgaagcca catttttgct
    24721 gcctggtacc ggaaaagcgt caggaagtaa caaccgaagg cggcctggat gtcgcagggc
    24781 agcgtgacaa aatgcgcgat gcctgcaaac gtctggcaga tgccgggatt caggtttctc
    24841 tgtttattga cgccgatgaa gagcagatca aagctgcggc agaggttggc gcaccgttta
    24901 tcgagatcca caccggttgc tatgctgatg ccaaaactga cgccgaacag gcgcaagagc
    24961 tggcgcgtat cgccaaagcc gcgacctttg ccgcaagcct cggtctgaaa gttaacgccg
    25021 gacacggtct gacctatcac aacgtgaaag ccattgccgc catccctgag atgcatgaac
    25081 tgaatatcgg tcatgccatt attggtcgtg cagtgatgac cggactgaaa gatgcggtgg
    25141 cagaaatgaa gcgtctgatg ctggaagcgc gtggctaatg gcaatattag gtttaggcac
    25201 ggatattgtg gagatcgctc gcatcgaagc ggtgatcgcc cgatccggtg atcgcctggc
    25261 acgccgcgta ttaagcgata acgaatgggc tatctggaaa acgcaccacc agccggtgcg
    25321 ttttctggcg aagcgttttg ctgtgaaaga agccgcagca aaagcgtttg gcaccgggat
    25381 ccgcaatggt ctggcgttta atcaatttga agtattcaat gatgagctcg gcaaaccacg
    25441 gctacggcta tggggcgagg cattaaaact ggcggaaaag ctgggcgttg caaatatgca
    25501 tgtaacgctg gcagatgagc ggcactatgc ttgtgccacg gtaattattg aaagttaaaa
    25561 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    25621 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    25681 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    25741 caatattgat ggaaagatta acgtgaccgc caattcgtaa gtacattaaa attggcttcg
    25801 ttattgaaga ttttgctgtg ctttacacca tgccacagaa ttcccccatt gaaacgagtg
    25861 gtgtcgtcaa agctctggtg tggagtgcag catgcaccct caataactcg cacgttcagt
    25921 tttggggaga tgtaagggct aatctgaatg gctgcattcc ttgtttaagg aaaaacgaat
    25981 gactgattgc cgatacctga ttaaacgggt catcaaaatc atcattgctg ttttacagct
    26041 gatccttctg ttcttataac acaaggaaac gtacttaagg tgcgtccggt gaaccagtcg
    26101 gacgcacctt taataactat aaataagt
//
LOCUS       DBBGLQ010000059        25322 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.059, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000059 DBBGLQ010000000
VERSION     DBBGLQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.059"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            312..1400
                     /gene="pdeL"
                     /locus_tag="ACIYSB_003349"
     CDS             312..1400
                     /gene="pdeL"
                     /locus_tag="ACIYSB_003349"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HGC5430331.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VNKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1502..2374)
                     /gene="yahB"
                     /locus_tag="ACIYSB_003350"
     CDS             complement(1502..2374)
                     /gene="yahB"
                     /locus_tag="ACIYSB_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGC5430332.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKK"
     gene            complement(2466..2963)
                     /gene="yahC"
                     /locus_tag="ACIYSB_003351"
     CDS             complement(2466..2963)
                     /gene="yahC"
                     /locus_tag="ACIYSB_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGC5430333.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGVISEYGGQKLSEATT
                     KRDGY"
     gene            3221..3826
                     /gene="yahD"
                     /locus_tag="ACIYSB_003352"
     CDS             3221..3826
                     /gene="yahD"
                     /locus_tag="ACIYSB_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGC5430334.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            3866..4729
                     /gene="yahE"
                     /locus_tag="ACIYSB_003353"
     CDS             3866..4729
                     /gene="yahE"
                     /locus_tag="ACIYSB_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGC5430335.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYLGLQRGKNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCV
                     LSQTYGGNYVS"
     gene            4719..6266
                     /gene="fdrA"
                     /locus_tag="ACIYSB_003354"
     CDS             4719..6266
                     /gene="fdrA"
                     /locus_tag="ACIYSB_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGC5430336.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIGSAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLDRGETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIETI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6266..7684
                     /gene="yahG"
                     /locus_tag="ACIYSB_003355"
     CDS             6266..7684
                     /gene="yahG"
                     /locus_tag="ACIYSB_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGC5430337.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7710..8030
                     /gene="yahH"
                     /locus_tag="ACIYSB_003356"
     CDS             7710..8030
                     /gene="yahH"
                     /locus_tag="ACIYSB_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
                     /protein_id="HGC5430338.1"
                     /translation="MNALSGLRMAQESVGLISVAHQAFVTIAGCGVNALSGLRMAQES
                     VGLISVAHQAFATTAGCGVDALSGLRVARESVGLISVAHQAFVTIAGCGVNALSGLRV
                     AREL"
     gene            8106..9056
                     /gene="yahI"
                     /locus_tag="ACIYSB_003357"
     CDS             8106..9056
                     /gene="yahI"
                     /locus_tag="ACIYSB_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGC5430339.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHKREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            9066..10448
                     /gene="yahJ"
                     /locus_tag="ACIYSB_003358"
                     /pseudo
     CDS             9066..10448
                     /gene="yahJ"
                     /locus_tag="ACIYSB_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            10664..10738
                     /locus_tag="ACIYSB_003359"
                     /pseudo
     CDS             10664..10738
                     /locus_tag="ACIYSB_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10825..11874
                     /gene="yahK"
                     /locus_tag="ACIYSB_003360"
     CDS             10825..11874
                     /gene="yahK"
                     /locus_tag="ACIYSB_003360"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGC5430340.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            12117..12932
                     /gene="yahL"
                     /locus_tag="ACIYSB_003361"
     CDS             12117..12932
                     /gene="yahL"
                     /locus_tag="ACIYSB_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGC5430341.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFHILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEYHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            13345..13590
                     /gene="yahM"
                     /locus_tag="ACIYSB_003362"
     CDS             13345..13590
                     /gene="yahM"
                     /locus_tag="ACIYSB_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HGC5430342.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGWGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAVIRQIRLIT"
     gene            complement(13607..14278)
                     /gene="yahN"
                     /locus_tag="ACIYSB_003363"
     CDS             complement(13607..14278)
                     /gene="yahN"
                     /locus_tag="ACIYSB_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGC5430343.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            14425..14700
                     /gene="yahO"
                     /locus_tag="ACIYSB_003364"
     CDS             14425..14700
                     /gene="yahO"
                     /locus_tag="ACIYSB_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGC5430344.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(14798..16384)
                     /gene="prpR"
                     /locus_tag="ACIYSB_003365"
     CDS             complement(14798..16384)
                     /gene="prpR"
                     /locus_tag="ACIYSB_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGC5430345.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFMQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            16623..17513
                     /gene="prpB"
                     /locus_tag="ACIYSB_003366"
     CDS             16623..17513
                     /gene="prpB"
                     /locus_tag="ACIYSB_003366"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGC5430346.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDNLFARSQVK"
     gene            17860..19029
                     /gene="prpC"
                     /locus_tag="ACIYSB_003367"
     CDS             17860..19029
                     /gene="prpC"
                     /locus_tag="ACIYSB_003367"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGC5430347.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAKHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            19063..20514
                     /gene="prpD"
                     /locus_tag="ACIYSB_003368"
     CDS             19063..20514
                     /gene="prpD"
                     /locus_tag="ACIYSB_003368"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGC5430348.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            20554..22440
                     /gene="prpE"
                     /locus_tag="ACIYSB_003369"
     CDS             20554..22440
                     /gene="prpE"
                     /locus_tag="ACIYSB_003369"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGC5430349.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            22770..24029
                     /gene="codB"
                     /locus_tag="ACIYSB_003370"
     CDS             22770..24029
                     /gene="codB"
                     /locus_tag="ACIYSB_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGC5430350.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            24019..25302
                     /gene="codA"
                     /locus_tag="ACIYSB_003371"
     CDS             24019..25302
                     /gene="codA"
                     /locus_tag="ACIYSB_003371"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGC5430351.1"
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
BASE COUNT         6226 a         6356 c         6826 g         5914 t
ORIGIN      
        1 gccagtatgt agtgggaaat tataaatatt gaaatagtcc aactacttct ttattaccaa
       61 aaatgagtat ctgaatttta atattgcatt cttgcgtgat tatctcctga gtttgacttg
      121 tgattacctt tttaaggtat ttagcgtaac tgtttttgag cgagcatcag aggtaaagat
      181 aatcttcttg atagtgatgt gggatgttat acgtatggca tcgctgatgt ttatggttac
      241 cccttatgtg tgctcgggaa tcgacaggta atcactcata ctgaacagcg ataaaagata
      301 aaggtgtgtt catgaattca tgtgattttc gtgtttttct gcaagagttc ggtacaacgg
      361 ttcatttgtc attgcctggt agcgtatccg agaaagaacg actgctactc aagctgctga
      421 tgcagggaat gtctgtaaca gaaatatcac agtacagaaa tcgcagtgca aagacaattt
      481 cacatcaaaa gaaacagctc tttgagaaac tggggattca gagcgatatt actttctggc
      541 gcgatatttt ctttcagtac aatccggaga tcatatccgc cacggggagt aatagtcaca
      601 gatatattaa tgataatcac tatcaccata tcgtcacgcc tgaagccatc agtctggcgt
      661 tggaaaacca cgaattcaaa ccgtggatcc aaccggtttt ctgcgcgcag actggggtac
      721 tgacgggctg tgaggtgctt gtccgctggg aacatccaca aacgggaatt atcccaccgg
      781 atcagtttat tcctctggcg gagtcatccg gtcttattgt cataatgacc cgccaactga
      841 tgaaacagac tgcggatatt ctgatgccgg taaaacattt gctgccggac aatttccata
      901 ttggcatcaa cgtctcggcg ggttgttttt tggcagcggg atttgaaaaa gagtgtctga
      961 acctggttaa taaattaggt aacgataaaa tcaagctggt tctcgagcta acggaacgta
     1021 accctattcc ggtaacgcca gaagccagag cgatatttga cagccttcat cagcacaaca
     1081 ttacctttgc gctggatgac tttggtacgg gttatgcgac ctatcgttac ttgcaggcgt
     1141 tcccggtcga ttttattaag atcgataagt catttgtgca aatggcgagt gtcgacgaaa
     1201 tctccggtca tattgtggac aatattgtcg aactagcgcg taagcctggt ctgagtatcg
     1261 tggcggaagg ggtagaaacc caggagcagg cggatttaat gatcggtaaa ggcgttcact
     1321 ttttgcaggg ctatttgtac tctccgccag taccgggtaa taaatttatc tctgaatggg
     1381 taatgaaagc aggtggttga tgtaaaccgc tattcacagc gcatcgggag gttggcagcg
     1441 attaattctc ccgatgcagt aaatcctgat agatccccgt taatatgcca tttggcgcaa
     1501 attatttttt aatccactgc gtcacctgac ccgtagcaga atgctgagtc gccagtaaca
     1561 tgcgcgaatc ctggcgcgga ttatggattt gtcgggtaac cagcagggat tgcgtcatcg
     1621 cctcacggac catgtaatcc ggcaaaaaac caattccttc acctaaaatc tggcactgac
     1681 atttggtgtt gaaatctggc accagaattg actcctgccc gtgcagcaac cagcccactt
     1741 ttttattaat cgtatgcgcg gtgtcctcca ccataatatt agggtagaga cgcagttggc
     1801 tttcggcgat gggttccggc acaaaggcta acgggtgatc cggggcgata gcaaaagccc
     1861 agcgaatcgc gccaatttca gtgtaatcaa taccgccgcc gtccagtagt gtatcgggtg
     1921 cgccgatggc gatattggcc tgattattaa taatcgcatc ccagacgccg ttatacactt
     1981 cggtggtgac ggtaatctgg caggtaggaa actgcttttt cagcacctgt aacaagcgtg
     2041 cggtgtgttt gggcgtataa agcagctgat taatacaaat gcgcacacgc gcttctatgc
     2101 cctgcgaaat agtatcgata ctgcgtttga tggcataaaa atcattcagc aggtcggtag
     2161 ctttgcggaa aaaataacgc ccggactccg ttaactcaat gctgcgggta ctgcgagtga
     2221 acagcaccac atccagcccc gtctccatac gcttaatggt gtagctaatg gcggaagtgg
     2281 ttaaacccaa ctcttctgcg gctttactga agctgccaaa acgcgcagcg gtagtaaatg
     2341 ccaacagatt ctcttcggta aaaattgagt tcattaaacc attctcgcca ttatctacta
     2401 tgaatttctc cagctatgac tgtagaggta tcggttaaag atagataatc atttttgaat
     2461 aacttttaat acccgtcgcg tttagtcgtc gcttcgctta atttttgtcc accatattct
     2521 gaaattaccc ccacgcagtt acccgccaga aaacccagta acgtcaccgt ccagttcatc
     2581 ccgctggcaa aaaacagcgt catgccgaga aagccaccgg gaataaagga cagtaaccag
     2641 aaacgccctt gccagaccac taccgccgag aagggtacgg tagtaattac gctcgcccag
     2701 aacgcggaca gtccacttac agaggccagc caaccagcag tcagcgcggc gaaaaacgcc
     2761 cacaccatcc cggaataatt aaccgccaga cttctgataa accccgattt accgccgcct
     2821 gcggcataaa aactgcaaaa ggcgacgaag ccaatggttc ctaataattc ccatccttga
     2881 gagagcccga catgtgaaga cactaactgc caaagccctg cacaaatacc gacggtgaca
     2941 cccgttgccg tgagtccgtt catcttgttc ccctctggtt agacatggac gaaagctact
     3001 cttacatgtg cagactatag agaaataaaa cctgattgag tgaaagggta tcgggtcaaa
     3061 gaaacaaata ttgcactacc gcacactgcg aaaagattgt tgaatattat tcaacaaaaa
     3121 ggcgagatac ggcgaattaa atttaagcgg gttcttttat cagattatcg ttgctattct
     3181 caattcatcg aaaacagcca caaaaaccag gggaatgtga atgagtataa aaaatctacc
     3241 tgccgactat ttattggctg cacaacaggg tgatatcgat aaagtaaaaa cctgccttgc
     3301 gctgggtgtc gatattaata cctgcgatcg tcaggggaaa acggcaatta cgctggcaag
     3361 tttatatcag caatatgctt gcgttcaggc attaattgat gccggagcgg atattaataa
     3421 gcaagatcat acctgtttaa atcctttttt aattagctgt ctgaacgatg atttaacgct
     3481 actacgaatt attttaccgg ctaaacccga tcttaattgc gtaacccgtt ttggcggtgt
     3541 cggcctgacg cctgcctgtg aaaaaggcca tttaagtatt gtaaaagagc ttttggcgca
     3601 tacggagatt aacgttaacc agaccaacca tgtaggctgg acgccgctac tggaagcgat
     3661 tgtgcttaat gatggtggta ttaaacagca ggcgattgtg cagttattgc tggaacacgg
     3721 tgccagcccg catctgaccg ataaatatgg caaaacgcca ctggaactgg cgcgggaacg
     3781 gggctttgaa gagattgcgc agttactgat tgccgcaggt gcataaaccg ggaggcttgc
     3841 tatcaacaca ccagaaagac ggtgtgtgtg ggcgctaact gcggatgcgg attttctggc
     3901 gcagcggggg caaggacagg ttgaacaggt ctttgccaga gcggtaaata tcgcactccc
     3961 ggctcgccag cagttgctga cgctgctttg tgaagagtac gacaatgcgc caaacagttg
     4021 tcggttggca ctcactcact ttgatgatct gttccggcat ggtgataagg ttcagtttga
     4081 cgatcaaggt attacggttg gtcaacatct tcatatagag atgagtcgtt gtcggcgttg
     4141 gctgtcccca accttgcaaa tgaccgctgt gaattttcac cttatcgcct ggctacagtg
     4201 gcacgacatt attcatcagc acctggggga aaatgaaacc ctgtttaatt atcgcggcga
     4261 taatccgttt tatcaggcgt taaataaaga attacatatt aaacgacggg cagttattca
     4321 ggccgtaaac gataaacaaa atatcgcctc agcggtcgcc agtatgatgg ggttagggat
     4381 tggccttacg ccatcagccg acgattattt aacaggtctg gcgcttattt tatttattcc
     4441 cgggcatccg gcggaaaaat acaaagagga attttatctc ggtctgcaac gcggcaaaaa
     4501 taataccaca ttattaagtg ccataacgct ggaagccgca ttacaacaac gctgccggga
     4561 aaatattcat cgttttattc acaacattat ttatgacatc cctgggaacg caactcaggc
     4621 aatagaaaaa attaaacata ttggctccag ttccggctgc gacatgctgt atggcatggc
     4681 cgatggttgt gtgctgagcc aaacctacgg agggaattat gtcagttaaa atagtcatta
     4741 aaccgaatac ctattttgat tctgtctcgc tgatgtctat ctccacgcgt gcaaataaac
     4801 tcgacggcgt cgagcaggca tttgtggcga tggcgaccga aatgaataaa ggcgtgctga
     4861 agaatttagg actgctgacg ccggagctgg agcaggcgaa aaacggcgac ctgatgattg
     4921 tcatcaatgg taaatcgggt gcggacaacg agcagttgct ggtggagatt gaagaactgt
     4981 tcaacaccaa agcgcaaagc ggctcgcacg aggcgcgtta cgccactatt ggcagcgcca
     5041 aaaagcatat cccggaaagt aacctggcgg tgatttcggt caacggtctg tttgccgctc
     5101 gcgaagcgcg tcaggcgctg caaaacgatc tcaacgtgat gctgttttcc gataacgtct
     5161 cagttgaaga tgaactggcg ctcaagcaac tggcccacga aaaagggctg ctgatgatgg
     5221 ggccagactg tggcacggcg attatcaacg gcgcggcgct ctgttttggt aacgccgtgc
     5281 gtcgcggcaa catcggtatt gttggcgcat ccggcaccgg cagtcaggag ttgagcgtcc
     5341 gcattcatga atttggcggc ggcgtttcgc aactgattgg caccggcggg cgcgacctga
     5401 gcgagaaaat cggcggcctg atgatgctcg acgccatcgg gatgctggaa aacgatccgc
     5461 aaactgaaat cattgcgctt atctccaaac cgcctgcgcc tgcggtggcc cgcaaagtgc
     5521 tggaacgtgc gcgcgcctgc cgcaagccgg tggtcgtctg cttcctcgat cgtggcgaaa
     5581 cgccagtgga tgagcagggg ctacagtttg cccgcggcac caaagaggca gcgctaaaag
     5641 cggtgatgct ctccggcgtg aaacaggaaa atctcgacct gcatacgctt aaccagccgt
     5701 tgattgcgga tgtgcgtgcg cgtctgcaac cgcagcagaa atacattcgt ggcctgttct
     5761 gcggcggcac gctgtgcgac gaaaccatgt tcgcggtgat ggaaaaacat ggcgatgtct
     5821 acagcaacat tcagcccgat ccggaattcc gcctgaaaga tatcaaccgc agcatcaaac
     5881 acaccttcct cgactttggc gatgacgact tcaccaacgg caagccgcac ccgatgattg
     5941 accccaccaa ccgcatcagt cgcttgatcg aagaggcgcg cgatccagaa gtggcggtga
     6001 tcgtgatgga ttttgtgctc ggatttggat cgcatgaaga tccggtcggc tccaccatcg
     6061 agacgatcaa agaagcgaaa gcgatcgccg ctgccgaagg acgcgagttg atcattctcg
     6121 cctatgtgct gggtaccgat cttgatacgc catcgttaga acaacaaagc cagatgctgc
     6181 ttgatgccgg agtgattctg gcgagcagca gcaccaatac cggattgctg gcgcgtgaat
     6241 ttatctgcaa aggggaggaa gcctgatgag ccagtcactg tttagccaac cattgaacgt
     6301 tattaacgtc ggcatcgcca tgtttagcga tgacctgaaa aagcagcatg tagaagtgac
     6361 tcaactcgac tggacgccgc cggggcaggg caatatgcag gtggtgcagg cgctggataa
     6421 cattgccgat tcgccgctgg cggacaaaat cgccgccgct aaccagcagg cgctggagcg
     6481 tattatccag tcgcatccgg tgctgattgg ttttgatcag gcgattaacg tggtgccggg
     6541 catgacggcg aaaaccattc ttcacgccgg gccgccgatc acctgggaaa aaatgtgcgg
     6601 cgcgatgaaa ggcgcggtca ccggagcgct ggtgttcgaa ggactggcga aagatctcga
     6661 cgaggcggct gaactggcgg cttccgggga gatcaccttc tcgccgtgtc acgagcacga
     6721 ctgcgtggga tcgatggcgg gtgttacctc ggcctcgatg tttatgcaca tcgtaaaaaa
     6781 caaaacctac ggcaacatcg cttataccaa catgagcgag cagatggcga agattttgcg
     6841 tatgggcgct aacgaccaga gcgtgattga ccgcctgaac tggatgcgtg atgtgcaggg
     6901 accaatactg cgcgacgcga tgaaaattat cggcgaaatc gatctgcgct taatgctggc
     6961 gcaggcgctg catatgggcg atgagtgcca taaccgcaat aacgccggga cgacactgct
     7021 gattcaggcg ctgacgccgg ggattattca ggcgggttat tccgtcgagc aacagcgcga
     7081 agtgtttgag tttgtcgcca gcagcgacta cttctccggc ccgacgtgga tggcgatgtg
     7141 taaagcggcg atggatgcgg cgcatggcat cgaatacagc accgtggtca ccaccatggc
     7201 gcgtaacggc gtcgagttcg gcctgcgggt cagcggcctg ccgggacaat ggtttaccgg
     7261 cccggcgcag caggtgatcg gcccgatgtt tgccggttat aagccggaag attcggggct
     7321 ggatatcggc gacagcgcca tcaccgaaac ctacggtatt ggcggatttg ctatggcgac
     7381 cgcgcccgct atcgtcgcgc tggtgggcgg cacggtggaa gaagctattg atttctcccg
     7441 tcagatgcgc gaaatcaccc tcggtgaaaa ccccaacgtc accattccgc tgctcggttt
     7501 tatgggcgtg ccgtcggcaa tcgacatcac ccgcgtgggt agcagcggca ttctgccggt
     7561 gatcaacacc gccatcgccc ataaagatgc gggcgtcggc atgattggcg cgggcattgt
     7621 gcatccacct tttgcctgct tcgagaaagc cattcttggc tggtgcgaac gttacggcgt
     7681 ctgacctaca tgttcatgcc ggatgcggcg tgaacgcctt atccggccta cgaatggcgc
     7741 aagaatctgt aggcctgata agcgtagcgc atcaggcatt tgtcaccatt gccggatgcg
     7801 gcgtgaacgc cttatccggc ctacgaatgg cgcaagaatc tgtaggcctg ataagcgtag
     7861 cgcatcaggc atttgccacc actgccggat gcggcgtgga cgccttatcc ggcctacgag
     7921 tggcgcgaga atctgtaggc ctgataagcg tagcgcatca ggcatttgtc accattgccg
     7981 gatgcggcgt gaacgcctta tccggcctac gagtggcgcg agaattgtag gcctgataag
     8041 cgtagcgcat caggcagtct ggcgttggtc ataaccccat caccctctgt agcggacata
     8101 acaacatgaa agagcttgtg gtcgttgcca ttggtggcaa cagcattatc aaagataacg
     8161 ccagccagtc gattgagcat caggcggagg cggtgaaagc cgtcgccgat acggtgctgg
     8221 aaatgctggc ttccgattac gacattgtgc tgacccacgg caacgggccg caggtcgggc
     8281 tggatttacg ccgtgcggag attgcccaca agcgcgaagg gctgccctta acgccgctgg
     8341 cgaactgtgt ggcagatacg cagggcggca tcggctatct gatccaacag gcgctgaata
     8401 accggctggc gcgtcacggc gagaagaaag ccgtcaccgt ggtgactcag gtggaagtgg
     8461 ataaaaacga tccaggtttt gcccatccca ccaagcccat cggcgcattc tttagtgaca
     8521 gccagcgtga cgaattacaa aaggcaaacc ctgactggtg ttttgttgaa gatgccgggc
     8581 ggggctatcg ccgcgtggtc gcctcgccgg aaccgaaacg tattgtcgaa gcacctgcca
     8641 ttaaagcgct gatccaacaa ggttttgtcg taattggcgc gggcggcggt ggaattccgg
     8701 tagtgcgtac tgacgcggga gattaccaaa gcgtggacgc ggttatcgac aaagatctct
     8761 ctaccgcgct gctggcccgt gaaattcacg ccgacattct tgtgatcacc actggcgtcg
     8821 aaaaagtgtg tattcacttt ggcaaaccgc agcagcaggc gctcgatcgg gtggatattg
     8881 ccaccatgac ccgctatatg caggaagggc atttcccgcc cggcagcatg ttgccaaaaa
     8941 tcatcgccag cctgacattt ttagaacaag gcggcaaaga agtgattatc accacgccgg
     9001 aatgcctgcc tgcggcgctg cgcggcgaaa cgggcactca tattattaaa acgtaaggac
     9061 gtaagatgaa agaaagcaat agccgccgtg aatttctgag ccagagcggt aagatggtca
     9121 ccgccgccgc gctgtttggt acctctgtgc cgctcgccca tgcggcggta gctggcaccc
     9181 taaactgcga agcgaacaac accatgaaaa tcactgaccc gcattactat ctcgataacg
     9241 tgctgctgga aaccggtttt gactacgaaa atggcgtggc ggtgcagacc cgcacggcgc
     9301 gccagaccgt ggagattcag gacggcaaaa ttgtcgccct gcgcgagaac aagctgcatc
     9361 cggacgccac gctgccgcac tatgacgctg gcggtaagct gatgctgccc accacccgcg
     9421 acatgcatat tcatctcgac aaaacctttt acggcgggcc gtggcgctcg ctcaatcgtc
     9481 cggcaggcac caccatccag gacatgatca aactctagca gaaaatgctg ccggaactgc
     9541 aaccgtacac tcaggagcgg gcagaaaaac tgattgattt attgcagtcg aaaggcacca
     9601 ccattgcccg cagccactgc aatatcgaac cggtttccgg cctgaaaaat ctgcaaaatt
     9661 tgcaggcggt gctggcgcga cgtcaggcgg gctttgagtg tgaaatcgtc gccttcccgc
     9721 agcacggttt gctgctgtcg aaatctgaac ctttaatgcg tgaagcgatg caggcggggg
     9781 cgcattacgt cggcggcctg gacccgacca gtgttgatgg cgcgatggaa aaatccctcg
     9841 acaccatgtt ccagattgcg ctggactacg acaaaggcgt cgatattcac ctgcacgaaa
     9901 ccactccggc aggcgtggca gccatcaatt atatggttga aacggtagag aaaacgccac
     9961 agctgaaggg caagctgacc atcagtcacg cctttgcgct ggcaacgctc aacgagcaac
    10021 aggtagatga actggcgaac cggatggtgg tgcaacaaat ttctatcgcc tcgacggtgc
    10081 cgattggcac gctgcatatg ccgctcaaac agttgcacga caaaggcgta aaagtgatga
    10141 ctggcactga cagcgttatc gaccactggt cgccttatgg tctgggcgac atgctggaaa
    10201 aagccaatct gtacgcgcag ctctatattc gtcctaacga acagaacctc tcccgctcgc
    10261 tgtttttagc cactggcgat gtattgccgc tgaatgaaaa aggcgagcgt gtatggccaa
    10321 aagcgcagga tgacgccagc tttgtgctgg tggacgcctc ttgttccgcc gaggcggtgg
    10381 cgcgtatctc gccgagaacc gcaacgttcc ataaagggca actggtgtgg gggagtgtgg
    10441 caggttgatg cgcatttgaa acgcctgcaa cggtgaatag taagagattt aagccccagg
    10501 gagtggggca atctgaatat gagtgtgtcg gcagaaatgg acattatctt tgaggaatat
    10561 gcccttattg acgttgtgta gataactatt tgtctacgtg aaaagatcat cagttttgcc
    10621 gattttagtc ttttacagat agcaaatatc acacttacag gcgctcgcct tatggggaac
    10681 gttgctgctc tgtttcattc aggctcgcgg catgttgggg ctggatcggg tggtttaaat
    10741 attggctata ttcaatggac gcgttttgcc gcgatgacat atcaggcgtt gccaaataca
    10801 catagctaat caggagtaaa cacaatgaag atcaaagctg ttggtgcata ttccgctaaa
    10861 caaccacttg aaccgatgga tatcacccgg cgtgaaccgg gaccgaatga tgtcaaaatc
    10921 gaaatcgctt actgtggcgt ttgccattcc gatctccacc aggtccgttc cgagtgggcg
    10981 gggacggttt acccctgcgt gccgggtcat gaaattgtgg ggcgtgtggt agccgttggt
    11041 gatcaggtag aaaaatatgc gccgggcgat ctggtcggtg tcggctgcat tgtcgacagt
    11101 tgtaaacatt gcgaagagtg tgaagacggg ttggaaaact actgtgatca catgaccggc
    11161 acctataact cgccgacgcc ggacgaaccg ggccatactc tgggcggcta ctcacaacag
    11221 atcgtcgttc atgagcgata tgttctgcgt attcgtcacc cgcaagagca gctggcggcg
    11281 gtggctcctt tgttgtgtgc agggatcacc acgtattcgc cgctacgtca ctggcaggcc
    11341 gggccgggta aaaaagtggg cgtggtcggc atcggcggtc tgggacatat ggggattaag
    11401 ctggcccacg cgatgggggc acatgtggtg gcatttacca cttctgaggc aaaacgcgaa
    11461 gcggcaaaag ccctgggggc cgatgaagtt gttaactcac gcaatgccga tgagatggcg
    11521 gctcatctga agagtttcga tttcattttg aatacagtag ctgcgccaca taatctcgac
    11581 gattttacca ccttgctgaa gcgtgatggc accatgacgc tggttggtgc gcctgcgaca
    11641 ccgcataaat cgccggaagt tttcaacctg atcatgaaac gccgtgcgat agccggttct
    11701 atgattggcg gcattccaga aactcaggag atgctcgatt tttgcgccga acatggcatc
    11761 gtggctgata tagagatgat tcgggccgat caaattaatg aagcctatga gcgaatgctg
    11821 cgcggtgatg tgaaatatcg ttttgttatc gataatcgca cactaacaga ctgaaaaaat
    11881 taataaatac cctgtggttt aacatattaa cttcgctctc cacttaactt tttagttaag
    11941 gagagcgaaa taatatcaaa gtagcagtaa aacctataac gtaaatttaa attgttaaat
    12001 taacgccctc cagtacacaa tacttcacac gttagttatg agcgatttct gatagtgcct
    12061 ggtttaatca gagctttatt atctgcgacg tttattttta tttaagagag atcgcgatga
    12121 tatcattaaa agctccgcac aataatttga tgccatatac gcaacaaagc atacttaata
    12181 cggttaaaaa caatcagtta ccagaggata tcaaaagctc cctggtttcc tgtgtcgata
    12241 tattcaaggt tttgattaaa caatattatg attatcctta tgattgtcgt gatgatttag
    12301 tcgatgacga caaactcatt catctcatgg ctgccgtacg agactgtgag tggtcagacg
    12361 ataacgcact caccataaat gtgcagttta atgatttccc tggattttat gactggatgg
    12421 attaccctga tcacccggtt aagtttgttt ttcacatact tgaaaatcaa aaagggacag
    12481 tttgggtata tgaccaggat gatgcgtttc ttgatattaa agcaaacgtg caggctggac
    12541 gctttaccgg gcttaagaaa ttagtgcagt ttatcgattc agtgcgaaca gattgcaaat
    12601 gtattcttct tgaataccat atgcctttgc tgagaatttt ccctaaaggg aaagagtgta
    12661 tgcatgtgga aaaatggctt cgcgaaatgt cgtctattcc ggagacagac gcgcccatta
    12721 agcaggcgct tgcgcatgga ttattattac acttaaaaaa tatctaccct gtttttcctg
    12781 aatcactggt gatgttattg ctctctgttc tggacgtaaa aacatacaga gatgatgcgc
    12841 gattaaatga atggatatct aatagagtgc aggaattagg tgacagatat tatcctgtaa
    12901 ataagcacgt taaaatccgc tacactcttt agttcagaaa gggttccacc tgcaagttgt
    12961 tattccacct gagagtgaat cgcaatgcag gtaatcattt catctgtata tctgtatgcc
    13021 aactggcatt ccatctcttt tttcgccgat attttgttgt gatcggcgac acttcgctta
    13081 aaaaagcacc agtagtggtt tcgcagccat gcggtgtata aaaaatgatc tcatgcagat
    13141 gttttgtgaa tgtgttggtt gacattcata tgaaaaaaat cataattcca tcatgtttgt
    13201 gtggggtctt ttctgtatct tacgcatcgc actcaagcct gacagaaaat atgctgtaag
    13261 gctcatatca aaaaccgcca ttagctcatc aggaagagca gacgacaacc aggattgttg
    13321 tatggtacgg ggttcgaggc ctcgatggcg gtccaacttt tcaaaactct tctgaatcag
    13381 atccccttat tatcatccct gcaaagtgga acattgccat tgtttggcta ttcaggatgg
    13441 ggcagaccaa tgaaaaaagc ccacacaggg gagagtgggc tgaaatggga agctaaagac
    13501 tcaagtaaac ttatcggaaa taaggaccac gcattacggg ggctatcatc gccgatggcc
    13561 gtgattcggc aaatcaggtt gattacgtag agagtaaatt atctgctcac cgctgcgtca
    13621 ccccttcgta aatcaggcgt agcgcgaata caccaataat tgcaccaata acccgactgg
    13681 caacgcgttg catacgccca taagcacgac gcacagcggg caaagaaaac gcctgactaa
    13741 gaaaaactcg ccagataatt gatgcgagca caatccccgc ccaggccatt aaacgtgccc
    13801 atgttggtgt ttcggcattt aatgttactg agaaaatact gataaaaaat aaaacggttt
    13861 gcgggttaga gagatcggta attaatccgc ggcgaaaaaa gacataccag ggggcgctaa
    13921 tcggttgttg tagtgtgctc atttgcggtg ttgactggcg gcgcatgctg caccacgcaa
    13981 accataagag ataagcgccg ccgacgattc tgataagcga aaaaatctcc tcacactgcg
    14041 taattagcgt tgcaagacca aacaaaccca acccggaata aaatgcatcg cccagcgcca
    14101 cgcccagccc ggtcagcacc cctgcgcgtc gaccggaagc caggctggtt tgtactacca
    14161 caaagagatt ggctcccgga ttaaaaaaag taatcacgaa cagtcctacg gtcaggtaaa
    14221 cggcatgcaa aggatccata gtgatttcat ccataaataa gtgaactaac tgcatcatat
    14281 ttctaccaaa aataatcggg tgcgagagag atcacaaagt gtcttatttc cggttactgg
    14341 cgtttatgcc ctgactgaac taattattaa tcaacccaat aatgtgggtg ggtgatagtg
    14401 tgataacaac tctggagccg taatatgaaa ataatctcta aaatgttagt cggtgcgtta
    14461 gcgttagccg ttaccaatgt ctatgccgct gaattgatga ccaaagcgga atttgaaaaa
    14521 gttgaatcgc agtatgaaaa aataggtgat atttcaacca gcaatgaaat gtcgactgca
    14581 gatgcaaaag aagatttgat caaaaaagcg gatgaaaaag gggctgatgt gttggtactg
    14641 acctccggtc aaactgacaa taagatccac ggcacggcaa atatttataa gaagaagtaa
    14701 ttctgaatcc tatgtaaaca tctccgatgc gtaagtttat cggtgatcat ctattgaaat
    14761 ttatgccgga taaagcgttc gcgctgcatt cggcagttca gcttttcagc cgccgccaga
    14821 acgtcgtccg gctgatgcct aaataattcg ccgctgctgt tttatcgcca ttaaatttct
    14881 ccagtgcctg ttgtggtgtc agtaagcgtg gagcgggagt tttcgccgac tcgcgcgcca
    14941 gttccggcag tagcagttgc ataaactgcg gcgttaaatc cggcgtcggt tccacactta
    15001 aaaacagcgc cagtcgttcc atcatattgc gcagttcacg aatattgcct ggccagtcgt
    15061 agtgcagcag cacagtttca cttgcctgta acccctggcg taatgcagca gaaaatgggg
    15121 cggagagcgc cgccagagac actttcaaaa agctttccgc cagcggaaga atatccgcca
    15181 cccgctcgcg cagtggtggc aattgcagac gcaaaatact cagccgataa aacagatcac
    15241 ggcgaaaacg tccttgctgc atatcttctt ccagattgca gtgagtggcg ctaatgaccc
    15301 gtacatctac cggaacaggc tgatgcccgc cgacgcgggt gacctctttt tcttccagca
    15361 cccgcagcag ccgggtctgc aaaggtagcg gcatttcgcc aatctcatcc agaaacagcg
    15421 taccgccgtg ggcaatttcg aacagcccgg cgcgacctcc gcgtcgcgag ccggtaaacg
    15481 ccccttcctc atagccaaac agttctgctt ccagcagcga ttcggcaatc gccccgcagt
    15541 tgacggcaac aaacggatgc gactttttgc cctgtcgcgc atcgtggcgg gcaaaatatt
    15601 cccgatgaat cgcctgggcc gccagctctt tgcccgtccc cgtttccccc tcaatcaaca
    15661 ccgccgcact ggagcgggca tacagcaaaa tagtctgccg tacttgttcc atctgtggtg
    15721 attgaccgag catatcgccc agcacgtaac gagtacgcag ggcgttgcgg gtggcatcgt
    15781 gagtgttatg gcgtaacgac atgcgcgtca tatccagcgc atcgctgaac gcctggcgca
    15841 cggtggcggc ggaatagata aaaattccgg tcattccggc ttcttctgcc aaatcggtaa
    15901 tcagccctgc gccgaccacc gcttcggtgc cgttagcttt tagctcgtta atctgcccgc
    15961 gtgcgtcttc ctcggtaatg tagctacgtt ggtcgaggcg caaattaaag gttttttgaa
    16021 acgccaccag cgctggaatg gtttcctgat aggtgacaac gccgatagaa gaggtgagtt
    16081 ttccggcttt tgccagtgcc tgtaacacat cgtagccgct cggtttaatc aaaataactg
    16141 gcactgacag gcggcttttc aggtacgcgc cgttagagcc agccgcgatg atggcgtcac
    16201 agcgttcgtt tgccagtttc ttgcggatgt aggtcactgc tttttcaaag ccaagctgaa
    16261 taggggtaat gttcgccagg tgatcaaact cgaggctgat atcgcgaaac agctcgaaca
    16321 ggcgcgttac agataccgtc cagataaccg gtttgtcgtc attaagccgt ggtggatgtg
    16381 ccatagcgca ccgcaaagtt aagaaaccga atattgggtt tagtcttgtt tcataattgt
    16441 tgcaatgaaa cgcggtgaaa cattgcctga aacgttaact gaaacgcata tttgcggatt
    16501 agttcatgac tttatctcta acaaattgaa attaaacatt taattttatt aaggcaattg
    16561 tggcacaccc cttgctttgt ctttatcaac gcaaataaca agttgataac aaaggatggg
    16621 ctatgtctct acactctcca ggtaaagcgt ttcgcgctgc actgactaaa gaaaatccat
    16681 tgcagattgt tggcaccatc aacgctaatc atgcgctgtt ggcgcagcgt gccggatatc
    16741 aggcaattta tctttctggc ggtggcgtgg cggcaggttc gctggggctg cccgatctcg
    16801 gtatttctac ccttgatgat gtgctgaccg acattcgccg tatcaccgac gtttgttcgc
    16861 tgccgctgct ggtggatgcg gatatcggtt ttggttcttc ggcctttaac gtggcgcgca
    16921 ccgtgaaatc gatgattaaa gccggtgcgg caggattgca tattgaagat caggttggtg
    16981 cgaaacgctg cggtcatcgt ccgaataaag cgatcgtctc gaaagaagag atggtggatc
    17041 ggatccgcgc ggcggtggat gcgaaaaccg atcctgattt tgtgatcatg gcgcgcaccg
    17101 atgctctggc ggtagagggg ctggatgcgg cgatcgagcg tgcgcaggcc tatgttgaag
    17161 cgggtgccga gatgttgttc ccggaggcga ttaccgaact cgccatgtac cgccagtttg
    17221 ccgatgcggt gcaggtgccg atcctcgcca acatcaccga atttggtgcc acgccgctgt
    17281 ttaccaccga cgaattacgc agcgcccatg tcgcaatggc gctgtaccca ctttcagcgt
    17341 tccgcgccat gaaccgcgcc gctgaacatg tctacaacgt cctgcgccag gaaggcacgc
    17401 agaaaagcgt catcgacacc atgcagaccc gcaacgagct gtacgaaagc atcaactact
    17461 accagtacga agagaagctc gacaacctgt ttgcccgtag ccaggtgaaa taaaaaacgc
    17521 ccgttgattg tattcgacag ccgatgcctg atgcgtcgct gacgcgactt atcaggccta
    17581 cgaggtgcac tgaactgtag gtcggataag acggatggcg tcgcatccga caaccgatgc
    17641 ctgatgcgcc gctgacgtga cttatcaggc ctacggggtg cactgaactg taggtcggat
    17701 aagacgcata gcgtcgcatc cgacaaccga tgcctgatgc gccgctgacg cgacttatca
    17761 ggcctacggg gtgaactgaa ctgtaggtcg gataagacgc atagcgtcgc atccgacaac
    17821 aatctcgacc ctacaaatga taacaatgac gaggacaaca tgagcgacac aacgatcctg
    17881 caaaacagta cccatgtcat taaaccgaaa aaatctgtgg cactttctgg cgttccggcg
    17941 ggcaatacgg cgctctgcac cgtgggtaaa agtggcaatg acctgcatta ccgcggctac
    18001 gatattcttg atctggcgaa acattgcgaa tttgaagaag tggcgcatct gctgatccac
    18061 ggcaaactgc cgacccgtga cgaactcgcc gcttacaaaa cgaaactgaa agccctgcgc
    18121 ggtttaccgg ctaacgtgcg taccgtgctg gaagccttac cggcggcgtc gcacccgatg
    18181 gatgttatgc gcaccggtgt ttccgcgctc ggctgcacgc tgccagaaaa agaggggcat
    18241 accgtctctg gcgcgcggga tattgccgac aaactgctgg cgtcgcttag ctcgattctc
    18301 ctttattggt atcactacag ccacaacggc gaacgcatcc aaccggaaac cgatgacgac
    18361 tccatcggcg gtcacttcct gcatctgctg cacggcgaaa agccatcgca aagctgggaa
    18421 aaggcgatgc atatctcgct ggtgctgtac gccgaacacg agtttaacgc ctccaccttt
    18481 accagtcggg tgattgcggg caccggctct gatatgtatt ccgcgattat tggcgcgatt
    18541 ggcgcactgc gcgggccaaa acacggcggg gcgaatgaag tgtcgctgga gatccagcaa
    18601 cgctacgaaa cgccggacga agccgaagca gatatccgca agcgcgtgga aaacaaagaa
    18661 gtggtcattg gttttggtca tccggtttac accatcgctg acccgcgcca ccaggtgatt
    18721 aaacgtgtgg cgaagcagct ctcgcaggaa ggcggctcgc tgaagatgta caacatcgcc
    18781 gatcgcctgg aaacggtgat gtgggagagc aaaaagatgt tccccaatct cgactggttc
    18841 tctgctgttt cctacaacat gatgggcgtt cccaccgaga tgttcacacc actgtttgtt
    18901 atcgcccgcg tcaccggctg ggcggcgcac attatcgaac aacgtcagga caacaaaatt
    18961 atccgtcctt ccgccaatta tgttggaccg gaagaccgcc cgtttgtcgc gctggataag
    19021 cgccagtaaa cctctacgaa taacaataag gaaacgtacc caatgtcagc tcaaatcaac
    19081 aacatccgcc cggaatttga tcgtgaaatc gttgatatcg tcgattacgt catgaactac
    19141 gaaatcagct ctaaagtggc ctacgacacc gcacattact gcctgctcga cacgctcggc
    19201 tgcggtctgg aagctctcga atacccggcc tgtaaaaaac tgctggggcc aattgttccc
    19261 ggcaccgtcg tacccaacgg cgtgcgcgtc cccggaactc agttccagct cgaccccgtc
    19321 caggcggcat ttaacatcgg cgcgatgatc cgctggctcg atttcaacga tacctggctg
    19381 gcggcggagt ggggccatcc ttccgacaac ctcggcggca ttctggcaac ggcggactgg
    19441 ctttcgcgca acgcggtcgc cagcggcaaa gcgccgttga ccatgaaaca ggtgctgacc
    19501 gcaatgatca aagcccatga aattcagggc tgcatcgcgc tggaaaactc ctttaaccgc
    19561 gtcggcctcg accacgttct gttagtgaaa gtggcttcca ccgccgtggt cgccgaaatg
    19621 ctcggcctga cccgcgagga aattctcaac gccgtttcgc tggcgtgggt ggacggtcag
    19681 tcgctgcgca cctatcgcca tgcgccgaac accggcacgc gtaaatcctg ggcggcgggc
    19741 gatgccactt cccgcgcggt acgtctggca ctgatggcga aaacgggcga aatgggttac
    19801 ccgtcagccc tgactgcgcc ggtgtggggc ttctacgacg tctcctttaa aggtgaatcg
    19861 ttccgcttcc agcgcccgta cggttcctac gttatggaaa atgtgctgtt caaaatctcc
    19921 ttcccggcgg agttccactc ccagacggca gttgaagcag cgatgacgct ctatgaacag
    19981 atgcaggcag caggcaaaac ggcggcggat atcgaaaaag tgaccattcg cacccacgaa
    20041 gcctgtattc gcatcatcga caaaaaaggg ccgctcaata acccggcaga ccgcgatcac
    20101 tgcattcagt acatggtggc gatcccgctg ctattcgggc gcttaacggc ggcagattac
    20161 gaggacaacg ttgcgcaaga taaacgcatt gacgccctgc gcgagaagat caattgcttt
    20221 gaagatccgg catttaccgc tgactaccac gacccggaaa aacgcgccat cgccaatgcc
    20281 attacccttg agttcaccga cggcacacga tttgaagaag tggtggtgga gtaccccatt
    20341 ggtcatgctc gccgccgtca ggatggtatt ccgaaactgg tcgataaatt caaaatcaat
    20401 ctcgcgcgcc agttcccgac tcgccaacag cagcgcattc tggaggtttc tctcgacaga
    20461 gctcgcctgg aacagatgcc ggtcaatgag tatctcgacc tgtacgtcat ttaagtaaac
    20521 ggcggtaagg cgtaagttca acaggagagc attatgtctt ttagcgaatt ttatcagcgt
    20581 tcgattaacg aaccggagca gttctgggcc gagcaggccc ggcgtattga ctggcagacg
    20641 ccctttacgc aaacgctcga tcacagcaat ccgccgtttg cccgttggtt ttgtgaaggc
    20701 cgaaccaact tgtgccacaa cgccatcgac cgctggctgg agaaacagcc agaggcgctg
    20761 gcgctgattg ccgtctcttc ggaaacagaa gaagagcgca cctttacctt tcgtcagctg
    20821 catgacgaag tgaacgcggt ggcctcaatg ttgcgttcat tgggtgtgca gcgcggcgat
    20881 cgggtgctgg tgtatatgcc gatgattgcc gaagcgcata ttactctgct ggcctgcgcg
    20941 cgcattggcg ctattcactc ggtggtgttt ggtggatttg cctcgcacag cgtggcggcg
    21001 cgaattgatg acgctaaacc ggtgctgatt gtctcggctg atgccggagc gcgcggtggc
    21061 aaaatcattc cctataaaaa attgctcgac gatgcgataa gtcaggcgca gcaccagcca
    21121 cgccatgttt tgctggtgga tcgcgggctg gcgaaaatgg cgcgcgtcag cgggcgggat
    21181 gtcgatttcg cgtcgttgcg ccatcaacac atcggcgcgc gggtaccggt ggcgtggctg
    21241 gaatccaacg aaacctcctg cattctctac acttccggca cgaccggcaa acctaaaggc
    21301 gtgcagcgtg acgtcggcgg atatgcggtg gcgctggcga cctcgatgga caccattttt
    21361 ggcggcaaag cgggcagcgt gttcttttgc gcatcggata tcggctgggt ggtggggcat
    21421 tcgtatatcg tttacgcgcc gctgctggcg gggatggcga ctatcgttta cgaaggattg
    21481 ccgacctggc cggactgcgg cgtgtggtgg aaaatcgtcg agaaatatca ggttagccgg
    21541 atgttctcag cgccgaccgc cattcgcgtg ctgaaaaaat tccctaccgc tgaaattcgc
    21601 aaacacgatc tctcgtcgct ggaagtgctc tatctggctg gagaaccgct ggacgagccg
    21661 accgccagtt gggtgagcaa tacgctggat gtgccggtca tcgacaacta ctggcagacc
    21721 gaatccggct ggccgattat ggcgattgct cgcggtctgg acgacaggcc gacgcgtctg
    21781 ggaagccccg gtgtgccgat gtatggctat aacgtgcagt tgcttaatga agtcaccggc
    21841 gaaccgtgtg gcgtcaacga gaaagggatg ctggtggtgg aagggccgct gccgccgggg
    21901 tgtattcaga ccatctgggg cgacgacggc cgctttgtga agacttactg gtcgctgttt
    21961 tcccgcccgg tgtacgccac ctttgactgg ggcatccgtg acgctgacgg ttatcacttt
    22021 attctcgggc gcactgacga tgtaattaac gttgccgggc atcggctggg gacgcgcgag
    22081 attgaagaga gtatctccag ccatccgggc gttgccgaag tggcggtggt tggggtgaaa
    22141 gatgcgctga aagggcaggt ggcggtggcg tttgtcattc cgaaagagag cgacagtctg
    22201 gaagatcgtg atgtggcgca ctcgcaagag aaggcgatta tggcgctggt ggacagccag
    22261 attggcaact ttggccgccc ggcgcacgtc tggtttgtct cgcaattgcc aaaaacgcga
    22321 tccggaaaaa tgctgcgccg cacgatccag gcgatttgcg aaggacgcga tcctggagat
    22381 ctgacgacca ttgatgatcc tgcgtcgttg gatcagatcc gccaggcgat ggaagagtag
    22441 gttattgtcg gatgcgtcgc gcggtgcatc cggcactgtg tgccgatgcc tgatgcgacg
    22501 ctgacgcgtt ttatcatgcc tacggacctg aaccgtaggt cggataaggc gctcgcgtcg
    22561 catccgacac catgctcaga tgcctgatgc gacgctgacg cgtcttatca ggcctaccca
    22621 ctgtttttac accgataatt tttcccccac ctttttgcac tcattcatat aaaaaatata
    22681 tttccccacg aaaacgattg ctttttatct tcagatgaat agaatgcggc ggattttttg
    22741 ggtttcaaac agcaaaaagg gggaatttcg tgtcgcaaga taacaacttt agccaggggc
    22801 cagtcccgca gtcggcgcgg aaaggggtat tggcattgac gttcgtcatg ctgggattaa
    22861 ccttcttttc cgccagtatg tggaccggcg gcactctcgg aaccggtctt agctatcatg
    22921 atttcttcct cgcagttctc atcggtaatc ttctcctcgg tatttacact tcatttctcg
    22981 gttacattgg cgcaaaaacc ggcctgacca ctcatcttct tgctcgcttc tcgtttggtg
    23041 ttaaaggctc atggctgcct tcactgctac tgggcggaac tcaggttggc tggtttggcg
    23101 tcggtgtggc gatgtttgcc attccggtgg gtaaggcaac cgggctggat attaatttgc
    23161 tgattgccgt ttccggttta ctgatgaccg tcaccgtctt ttttggcatt tcggcgctga
    23221 cggttctttc ggtgattgcg gttccggcta tcgcctgcct gggcggttat tccgtgtggc
    23281 tggctgttaa cggcatgggc ggcctggacg cattaaaagc ggtcgttccc gcacaaccgt
    23341 tagatttcaa tgtcgcgctg gcgctggttg tggggtcatt tatcagtgcg ggtacgctca
    23401 ccgctgactt tgtccggttt ggtcgcaatg ccaaactggc ggtgctggtg gcgatggtgg
    23461 cctttttcct cggcaactcg ttgatgttta ttttcggtgc agcgggcgct gcggcactgg
    23521 gcatggcgga tatctctgat gtgatgattg ctcagggcct gctgctgcct gcgattgtgg
    23581 tgctggggct gaatatctgg accaccaacg ataacgcact ctatgcgtcg ggtttaggtt
    23641 tcgccaacat taccgggatg tcgagcaaaa ccctttcggt aatcaacggt attatcggta
    23701 cggtctgcgc attatggctg tataacaatt ttgtcggctg gttgaccttc ctttcggcag
    23761 ctattcctcc agtgggtggc gtgatcatcg ccgactatct gatgaaccgt cgccgctatg
    23821 agcactttgc gaccacgcgt atgatgagtg tcaattgggt ggcgattctg gcggtcgcct
    23881 tggggattgc tgcaggccac tggttaccgg gaattgttcc ggtcaacgcg gtattaggtg
    23941 gcgcgctgag ctatctgatc cttaacccga ttttgaatcg taaaacgaca gcagcaatga
    24001 cgcatgtgga ggctaacagt gtcgaataac gctttacaaa caattattaa cgcccggtta
    24061 ccaggcgaag aggggctgtg gcagattcat ctgcaggacg gaaaaatcag cgccattgat
    24121 gcgcaatccg gcgtgatgcc cataactgaa aacagcctgg atgccgaaca aggtttagtt
    24181 ataccgccgt ttgtggagcc acatattcac ctggacacca cgcaaaccgc cggacaaccg
    24241 aactggaatc agtccggcac gctgtttgaa ggcattgaac gctgggccga gcgcaaagcg
    24301 ttattaaccc atgacgatgt gaaacaacgc gcatggcaaa cgctgaaatg gcagattgcc
    24361 aacggcattc agcatgtgcg tacccatgtc gatgtttcgg atgcaacgct aactgcgctg
    24421 aaagcaatgc tggaagtgaa gcaggaagtc gcgccgtgga ttgatctgca aatcgtcgcc
    24481 ttccctcagg aagggatttt gtcgtatccc aacggtgaag cgttgctgga agaggcgtta
    24541 cgcttagggg cagatgtagt gggggcgatt ccgcattttg aatttacccg tgaatacggc
    24601 gtggagtcgc tgcataaaac cttcgccctg gcgcaaaaat acgaccgtct catcgacgtt
    24661 cactgtgatg agatcgatga cgagcagtcg cgctttgtcg aaaccgttgc tgccctggcg
    24721 caccatgaag gcatgggcgc gcgagtcacc gccagccaca ccacggcaat gcactcctat
    24781 aacggggcgt atacctcacg cctgttccgc ttgctgaaaa tgtccggtat taactttgtc
    24841 gccaacccgc tggtcaatat tcatctgcaa ggacgtttcg atacgtatcc aaaacgtcgc
    24901 ggcatcacgc gcgttaaaga gatgctggag tccggcatta acgtctgctt tggtcacgat
    24961 gatgtcttcg atccgtggta tccgctggga acggcgaata tgctgcaagt gctgcatatg
    25021 gggctgcatg tttgccagtt gatgggctac gggcagatta acgatggcct gaatttaatc
    25081 acccaccaca gcgcaaggac gttgaatttg caggattacg gcattgccgc cggaaacagc
    25141 gccaacctga ttatcctgcc ggctgaaaat gggtttgatg cgctgcgccg tcaggttccg
    25201 gtacgttatt cggtacgtgg cggcaaggtg attgccagca cacaaccggc acaaaccacc
    25261 gtatatctgg agcagccaga agccatcgat tacaaacgtt gaacgactgg gttacagcga
    25321 gc
//
LOCUS       DBBGLQ010000060        24978 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.060, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000060 DBBGLQ010000000
VERSION     DBBGLQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.060"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(252..2825)
                     /gene="clpB"
                     /locus_tag="ACIYSB_003372"
     CDS             complement(252..2825)
                     /gene="clpB"
                     /locus_tag="ACIYSB_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGC5430352.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2955..3686)
                     /gene="yfiH"
                     /locus_tag="ACIYSB_003373"
     CDS             complement(2955..3686)
                     /gene="yfiH"
                     /locus_tag="ACIYSB_003373"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGC5430353.1"
                     /translation="MSKLIVPQWPQPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGGEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3683..4663)
                     /gene="rluD"
                     /locus_tag="ACIYSB_003374"
     CDS             complement(3683..4663)
                     /gene="rluD"
                     /locus_tag="ACIYSB_003374"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGC5430354.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4798..5535
                     /gene="bamD"
                     /locus_tag="ACIYSB_003375"
     CDS             4798..5535
                     /gene="bamD"
                     /locus_tag="ACIYSB_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGC5430355.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5806..6147
                     /gene="raiA"
                     /locus_tag="ACIYSB_003376"
     CDS             5806..6147
                     /gene="raiA"
                     /locus_tag="ACIYSB_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGC5430356.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6250..6375
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6251..6298
                     /gene="pheL"
                     /locus_tag="ACIYSB_003377"
     CDS             6251..6298
                     /gene="pheL"
                     /locus_tag="ACIYSB_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HGC5430357.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            6397..7557
                     /gene="pheA"
                     /locus_tag="ACIYSB_003378"
     CDS             6397..7557
                     /gene="pheA"
                     /locus_tag="ACIYSB_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGC5430358.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7600..8721)
                     /gene="tyrA"
                     /locus_tag="ACIYSB_003379"
     CDS             complement(7600..8721)
                     /gene="tyrA"
                     /locus_tag="ACIYSB_003379"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGC5430359.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLVAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8732..9802)
                     /gene="aroF"
                     /locus_tag="ACIYSB_003380"
     CDS             complement(8732..9802)
                     /gene="aroF"
                     /locus_tag="ACIYSB_003380"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGC5430360.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKSI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10012..10377
                     /gene="yfiL"
                     /locus_tag="ACIYSB_003381"
     CDS             10012..10377
                     /gene="yfiL"
                     /locus_tag="ACIYSB_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGC5430361.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10527..11045
                     /gene="yfiR"
                     /locus_tag="ACIYSB_003382"
     CDS             10527..11045
                     /gene="yfiR"
                     /locus_tag="ACIYSB_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGC5430362.1"
                     /translation="MRFSHRLFLLLILLLTGAPILALEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11035..12261
                     /gene="dgcN"
                     /locus_tag="ACIYSB_003383"
     CDS             11035..12261
                     /gene="dgcN"
                     /locus_tag="ACIYSB_003383"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGC5430363.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12277..12759
                     /gene="yfiB"
                     /locus_tag="ACIYSB_003384"
     CDS             12277..12759
                     /gene="yfiB"
                     /locus_tag="ACIYSB_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGC5430364.1"
                     /translation="MIKHLVAPLVFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12835..13182)
                     /gene="rplS"
                     /locus_tag="ACIYSB_003385"
     CDS             complement(12835..13182)
                     /gene="rplS"
                     /locus_tag="ACIYSB_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGC5430365.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13224..13991)
                     /gene="trmD"
                     /locus_tag="ACIYSB_003386"
     CDS             complement(13224..13991)
                     /gene="trmD"
                     /locus_tag="ACIYSB_003386"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGC5430366.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14022..14570)
                     /gene="rimM"
                     /locus_tag="ACIYSB_003387"
     CDS             complement(14022..14570)
                     /gene="rimM"
                     /locus_tag="ACIYSB_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGC5430367.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVNDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14589..14837)
                     /gene="rpsP"
                     /locus_tag="ACIYSB_003388"
     CDS             complement(14589..14837)
                     /gene="rpsP"
                     /locus_tag="ACIYSB_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGC5430368.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15086..16447)
                     /gene="ffh"
                     /locus_tag="ACIYSB_003389"
     CDS             complement(15086..16447)
                     /gene="ffh"
                     /locus_tag="ACIYSB_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGC5430369.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16614..17405
                     /gene="ypjD"
                     /locus_tag="ACIYSB_003390"
     CDS             16614..17405
                     /gene="ypjD"
                     /locus_tag="ACIYSB_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGC5430370.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17426..18712
                     /gene="yfjD"
                     /locus_tag="ACIYSB_003391"
     CDS             17426..18712
                     /gene="yfjD"
                     /locus_tag="ACIYSB_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGC5430371.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18767..19360)
                     /gene="grpE"
                     /locus_tag="ACIYSB_003392"
     CDS             complement(18767..19360)
                     /gene="grpE"
                     /locus_tag="ACIYSB_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGC5430372.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19483..20361
                     /gene="nadK"
                     /locus_tag="ACIYSB_003393"
     CDS             19483..20361
                     /gene="nadK"
                     /locus_tag="ACIYSB_003393"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGC5430373.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20447..22108
                     /gene="recN"
                     /locus_tag="ACIYSB_003394"
     CDS             20447..22108
                     /gene="recN"
                     /locus_tag="ACIYSB_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGC5430374.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22257..22598
                     /gene="bamE"
                     /locus_tag="ACIYSB_003395"
     CDS             22257..22598
                     /gene="bamE"
                     /locus_tag="ACIYSB_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGC5430375.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22660..22950)
                     /gene="yfjF"
                     /locus_tag="ACIYSB_003396"
     CDS             complement(22660..22950)
                     /gene="yfjF"
                     /locus_tag="ACIYSB_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGC5430376.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22940..23377)
                     /gene="ratA"
                     /locus_tag="ACIYSB_003397"
     CDS             complement(22940..23377)
                     /gene="ratA"
                     /locus_tag="ACIYSB_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGC5430377.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23548..24030
                     /gene="smpB"
                     /locus_tag="ACIYSB_003398"
     CDS             23548..24030
                     /gene="smpB"
                     /locus_tag="ACIYSB_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGC5430378.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24245..24607
                     /gene="ssrA"
                     /locus_tag="ACIYSB_003399"
     tmRNA           24245..24607
                     /gene="ssrA"
                     /locus_tag="ACIYSB_003399"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24879..>24978
                     /locus_tag="ACIYSB_003400"
     CDS             24879..>24978
                     /locus_tag="ACIYSB_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HGC5430379.1"
                     /translation="MRIEICIAKEKFSKMPKGSVPALQQEMSRRIRK"
BASE COUNT         6345 a         6601 c         5873 g         6159 t
ORIGIN      
        1 ttcccgctac agagtcaagc atttctttgc ttttctctgt tgagattctc aggagaaccc
       61 cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt ggtggcgcat tatagggagt
      121 tattccggcc tgacaagcga aaaatataaa aactttatcg ttcgctcact tttcaggcaa
      181 aacatcttaa atatagtctt ttccgtctaa cttatagaca aaaacgagcc ccgaagggct
      241 cgttttatca tttactggac ggcgacaatc cggtcttcat taacttccag gcgaatcact
      301 ttacccggaa ccaattcacc agacagtatt tgctgtgcca gcgggttttc gatctgctgc
      361 tgaattgcac gtttcagagg acgtgcacca tagaccggat cgtaaccgtt ctcgctcagc
      421 agtttcagcg cctcgtcaga aatgtggatt tcataaccac gttcttccag acgtttgtac
      481 agacgtttca actgaatctg cgcaatcgag gcaatgtgct gttcacccag cggatggaag
      541 accaccactt catcgatacg gttaatgaat tccggacgga agttatggct taccacaccg
      601 agcaccagct ctttcatgtg cgcataatcc agttcaccga agcgttcctg aatcagatcg
      661 gaaccgaggt tagaggtcat aatgacgacc gtattacgga agtcgaccgt tctcccttgc
      721 ccgtcagtca gacgcccatc atccagtacc tgcaacagaa tgttgaagac atccggatgc
      781 gctttttcca cttcatccag caggatgacg gaatacggac gacgacgcac cgcttcggtc
      841 aggtagccac cttcttcata accgacatat cccggaggcg caccaaccaa acgagacacc
      901 gagtgtttct ccataaactc ggacatatcg atacggacca tcgcctcgtc gctatcaaac
      961 ataaagttcg ccagcgcctt acaaagctct gttttcccca caccagttgg gccgaggaac
     1021 aggaatgaac caatcgggcg atttggatcc gccagccccg cacggctacg acgaatagcg
     1081 ttagataccg catcaaccgc ttcgttctga ccaattacgc gatggtgcag ttcttgctcc
     1141 atacgcagca gtttttcgcg ctcgctttcc atcatgcgag aaaccggaat ccccgtccaa
     1201 cgcgccagca cttcagcaat ttcggcgtcg gtcactttat tacgcaacag acgcatagtt
     1261 ttgccttcga gctgcgttgc ggcttccagt tgcttttcca gttccgggat tttgccgtat
     1321 tgcagttcag acatccgcgc caggtccccc acacggcgag cctgttcaat agcgattttc
     1381 gcctgttcca gttccgcttt aatggtctgc gtaccagaaa gcgatgcctt ctctgctttc
     1441 cactcttctt ctaactcgga gtactgacgt tctttgtcgc tcagttcttc gttgagcata
     1501 tccagacgtt ttttactggc ttcatcagac tctttcatta acgcctgttg ttccagtttg
     1561 agctggatga tacgacgatc gagtcggtcg agttcttctg gttttgagtc aatctgcata
     1621 cgaatgctgg atgctgcttc atcgatcagg tcgatggctt tatccggcag ctgacggtca
     1681 gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg ccgggtcagt aatttgcaca
     1741 tggtggtgca attcgtaacg ttctttcagg ccacgcagaa tcgcaatggt atcttcaaca
     1801 gaaggctcgg caacaaacac tttctggaaa cgacgttcca gcgcagcatc tttttcaatg
     1861 tactggcgat attcgtcaag cgtcgtggca cctacgcagt gcaattcacc acgcgccagc
     1921 gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg ctttacccgc gccgaccatg
     1981 gtatgtaatt cgtcgataaa taggatgacg ttgccttcct gtttggcaag atcgttaagc
     2041 acgcctttta aacgttcttc aaactcaccg cgatatttcg ccccagccac cagcgcgccc
     2101 atatccagcg ccagtacccg gcggcctttc aacccttccg gcacttcgcc gttgataata
     2161 cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc cgggttcacc aatcagtacc
     2221 gggttatttt tagtacgacg ttgcagcacc tgaatggtac ggcgaatttc ttcatcacga
     2281 ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg taaggtcgat ggtatatttt
     2341 ttcaaagcct gacgttggtc ttcagcacct tgatcgttca cgctttcacc tccacgcatt
     2401 tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg ctgctttcag gatgtcggcc
     2461 agcgtgccgc gagactcaag tgccgccaga acgaacagtt ctgacgagat aaagttatca
     2521 ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc gcaccagatc ctgtgatggc
     2581 tggacatcac caccagtacc ttcaacctgc ggtaaacgat ttaatgcctg attgatatct
     2641 gtgcgcaact ggccagcatt tatgccagcg gatgttaata aaggactaac cgaaccccct
     2701 tcctgattca gcagggcgct cattaaatga agtggttcga taaattggtt gtcgtgcccg
     2761 agtgcaagtg attgggcatc ggcaagagca agctggaatt tattagtaag acgatccaga
     2821 cgcataactc ctcccataac ggatcaaagt tgctactgga gattaaatga ggtcatccct
     2881 caattattca aggttattga ccagattaat gtgaaaagaa aatcacgcgt accggatcgt
     2941 cttgattctt taggttatat cagccaaatg aaacttgcca tacgaccggt ggtcttgtcg
     3001 cgacgataag agaagaaagt ctcattttcc gtatatgtac aacggtcgcc gccgaaaatt
     3061 tgctcaacac ccacgttcgc cagacgctgc cgggcaagct gataaatatc cgccagatac
     3121 ttatcaccat gctgaatgaa agctgcactt gctttagcgt ctactgccat aaacgcctcg
     3181 cgaacctccc cccccacttc gaacgcgcgt ggaccaattg ccggccctaa ccaggcgaga
     3241 atattttccg gattatcagc aaaacaggaa accgtctctt ccagcacgcc tgcgcacagt
     3301 ccacgccagc cagcatgagc ggcggcgact tccgttcccg ctcgattgca aaacagcaca
     3361 gggaggcagt cggcagtcat cactgcgcaa accgtgccgg gcgtattgct ataagaggca
     3421 tccgcccgtt ttgaggcata aggttcgcca gtgagcttaa gcacatcttt gccgtgtacc
     3481 tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag caaaaagtcg cttgcgattc
     3541 tcctcaacgt gatccgggtt atcgccacaa tgggcaccga ggttgagtga gtcatacggg
     3601 ggcaagctca cgccgccgat acgagtggag ctacaggccg caacaccttt tggctgcggc
     3661 cactgcggga caatcagctt actcataacc agtccacttc atccttatgt tcttcgaaat
     3721 cggcgcgcat cacctcaatc agctccacca tatcttgtgg aataggcgca tgccattcca
     3781 tttcgatgcc ggagatcggg tgataaagac gcagcatggt tgcatgtagc gcctggcggt
     3841 caaacttacg cagcgtggag ataaatgctt ccgaagcacc ttttggcgga cgcggacggc
     3901 caccataaac cggatcgccc accagcggat gagtgatatg ggccatatgc acgcggatct
     3961 ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt gtgcacacgg aagtgttcca
     4021 tgatgcgata gtgagtcacc gctggtttgc ccatcggatg caccgccata tgggtacgtt
     4081 tggtcgggtg gcgactgatt ggctcgtcca ccgtgccacc tgcggtcata tgaccaatcg
     4141 ccaccgcttc atactcacga gtaatttcac gccgttgcaa agattcgact aaacgcgtct
     4201 gagccggaac ggtttttgcc acaaccatca ggccagtggt gtctttatcc agacgatgga
     4261 cgatgcccgc acgcggtaca tcggcaatgg gtggatagta atgaagcaac gcattcagta
     4321 ccgtgccatc cgggttaccc gcgccaggat gtaccaccag gtcgcgcggt ttattaatga
     4381 taataatgtc ttcatcttca tagacgatat ccagcgggat atcctgcggt tcaaaacgcg
     4441 cttcttcttc aatctcagcg ttgatggcaa cctgctcgcc acccaatact ttttctttcg
     4501 gcttatcaca aactttgccg ttaaccagca ctcgctggtc gaggatccat tcttttattc
     4561 gcgaacgtga ataatccggg aacatttcgg ccaaagcctg atctaagcgt tgaccgagtt
     4621 ggttttcgga caccgttgca gtgagctgta ctcgttgtgc catatatact gcttcttcgt
     4681 ttatcgttgg ggttttacgg ctttgccgtt taatatagtg tgctattgta gctggtctta
     4741 accgggagca ggaacagaga atctcccgta ttacattttg aggaaagtca aaacgtcatg
     4801 acgcgcatga aatatctggt ggcagccgcc acactaagcc tgtttttggc gggttgctcg
     4861 gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa tttacgcgac tgcacaacaa
     4921 aagctgcagg acggtaactg gagacaggca ataacgcaac tggaagcgtt agataatcgc
     4981 tatccgtttg gtccgtattc gcagcaggtg cagctggatc tcatctacgc ctactataaa
     5041 aacgccgatt tgccgttagc acaggctgcc atcgatcgtt ttattcgcct taacccgacc
     5101 catccgaata tcgattatgt catgtacatg cgtggcctga ccaatatggc gctggatgac
     5161 agtgcgctgc aagggttctt tggcgtcgat cgtagcgatc gcgatcctca acatgcacga
     5221 gctgcgttta gtgacttttc caaactggtg cgcggctatc cgaacagtca gtacaccacc
     5281 gatgccacca aacgtctggt attcctgaaa gatcgtctgg cgaaatatga atactccgtg
     5341 gccgagtact atacagaacg tggcgcatgg gttgccgtcg ttaaccgcgt agaaggcatg
     5401 ttgcgcgact acccggatac ccaggctacg cgtgatgcgc tgccgctgat ggaaaatgca
     5461 taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag cgaaaatcat cgccgcaaac
     5521 agcagcaata cataacagaa acctgaaaca caaaacggca gcccttgagc tgccgttttt
     5581 ttattctgtc agttgtgaaa ctgaagcgat ttagtcgcta tcgatctcat caaatatggc
     5641 tcgctttgag atattcctca agtaaaaaaa catctcttcc tgcgatttct cacaaaaaag
     5701 attcgttgac aaaaagtgac aaaattatga gattttcatc acacattttg acatcaggaa
     5761 cggtatgctg aattcaccaa gacgggaaga caagaggtaa aatttatgac aatgaacatt
     5821 accagcaaac aaatggaaat tactccggcc atccgccaac atgtcgcaga ccgtctcgcc
     5881 aaactggaaa aatggcaaac acatctgatt aatccacata tcattctgtc caaagagcca
     5941 caagggtttg ttgctgacgc cacaatcaat acacctaacg gcgttctggt tgccagtggt
     6001 aaacatgaag atatgtacac cgcaattaac gaattgatca acaagctgga acggcagctc
     6061 aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac
     6121 ttcgtcgaag aagttgaaga agagtagtcc tttatattga gtgtatcgcc aacgcgcctt
     6181 cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt actgtactaa agtcacttaa
     6241 ggaaacaaac atgaaacaca taccgttttt cttcgcattc ttttttacct tcccctgaat
     6301 gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa caataaggcc tcccaaatcg
     6361 gggggccttt tttattgata acaaaaaggc aacactatga catcggaaaa cccgttactg
     6421 gcgctgcgag agaaaatcag cgcgctggat gaaaaattat tagcgttact ggcagaacgg
     6481 cgcgaactgg ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgtgat
     6541 attgatcgtg aacgcgattt gctggaaaga ttaattacgc tcggtaaagc gcaccatctg
     6601 gacgcccatt acattactcg cctgttccag ctcatcattg aagattccgt attaactcag
     6661 caggctttgc tccaacaaca tctcaataaa attaatccgc actcagcacg catcgctttt
     6721 ctcggcccca aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt
     6781 gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc
     6841 ggccaggccg actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaacgac
     6901 gtttacgatc tgctgcaaca taccagcttg tcgattgttg gcgagatgac gttaactatc
     6961 gaccattgtt tgttggtctc cggcactact gatttatcca ccatcaatac ggtctacagc
     7021 catccgcagc cattccagca atgcagcaaa ttccttaatc gttatccgca ctggaagatt
     7081 gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat
     7141 gttgctgcgt tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt
     7201 attgaagcaa atcagcgaca aaacttcacc cgatttgtgg tgttggcgcg taaagccatt
     7261 aacgtgtctg atcaggttcc ggcgaaaacc acgttgttaa tggcgaccgg gcaacaagcc
     7321 ggtgcgctgg ttgaagcgtt gctggtactg cgcaaccaca atctgattat gacccgtctg
     7381 gaatcacgcc cgattcacgg taatccatgg gaagagatgt tctatctgga tattcaggcc
     7441 aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt taggggaaat cacccgttca
     7501 atgaaggtat tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga
     7561 aaaggtgccg gatgatgtga atcatccggc actggattat tactggcgat tgtcattcgc
     7621 ctgacgcaat aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg
     7681 ctccaccttg cggaaactgt caataaacgc ctgcttatcg ccctgctcca gcaactcaat
     7741 cgcctcgccg aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat
     7801 aatgatgtcg gcataaagct gcggatcctg agcaaacagt cgcccgacca tcgccagctc
     7861 aaggcggtaa atcggcgaag agagcgccag aagttgctca agctgaacat tttcttctgc
     7921 caggtgcagc ccgtaagcaa aagtagcaaa gtggcgcagt gcctgaataa acgccatatt
     7981 ctgatcgtgc tcgacggcgc taatacgatg cagccgagcg ccccagacct gaatttgctc
     8041 cagaaaccat tggtatgctt ccggtttacg tccatcacac cagaccacaa cttgctttgc
     8101 caggctaccg ctgtccggac cgaacatcgg gtgtagcccc agcaccggac catcatgcgc
     8161 caccagcatg gcctgtaatg gcccattttt cactgatgcc agatcgacca gaatacaatc
     8221 tttcggtaaa ggcggtaatt tgccaataac ttgctcagta acgtggattg gcacactaac
     8281 aatcaccatt ccggcatcgg caacaatatc agccgctcga tcccagtcat gttgctccag
     8341 aatccgcacc tgataacccg agagggtcag catcttctcg aacaggcgtc ccatctgacc
     8401 gccaccgccg acgataacca ccggacgcag tgacggacaa agtgttttaa atcctttgtc
     8461 gttttcactg gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg
     8521 cggtacaccc agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc
     8581 cggaacataa ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc
     8641 cagacgcttc gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa
     8701 tgcggtcaat tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt
     8761 tcagatcctg atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat
     8821 cggttacgga tacaccgtat ttcatttcac tgcgcggttg ctcggaagac tgattgccct
     8881 cgtggatatt actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag
     8941 caaccacgga ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc
     9001 tgcaatctac catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg
     9061 caacatccgc agggctatag ttcggcgctt taccaccgcg caggatcaca tggccgtccg
     9121 gattcccctg agtttgtagc aacgcaacct gccctgcctg gttaatgcca acaaaacggt
     9181 gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc cagactgccg tcggtgccgt
     9241 ttttaaaacc aaccggcatg gaaagcccgg aggccatttc acggtgagtt tgcgattccg
     9301 ttgtacgagc accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg
     9361 gatctaacgc ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac
     9421 gcgcgatctg cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatta
     9481 accctttcca gccgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga
     9541 ggctatcgct gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt
     9601 ccggatcatg aatggaacaa ggaccacata ctaccagcag acgaggatcg cgcccggcga
     9661 taatatctga aatgctttta cgcgagtcag caatctgggc ttcttgttgc aggctcaatg
     9721 gaaaagcggc cttcagttgt tccggagtca ttaaaacctg ttcgtcggta atatgtacgt
     9781 tattcagcgc gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat
     9841 cctcagcgag gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta
     9901 catttttatt tacacaggca atttagtcgc gctttcaacc cttacctctg tatagataaa
     9961 tttacactcc ctttgaaaac aatccgctat gctttgaaaa aggagaaaga aatgatgaaa
    10021 aagtttatcg cccccttgtt ggctttactg gttagcggat gtcagattga tccttatact
    10081 cacgcgccaa ccttgaccag caccgactgg tatgatgtcg gtatggaaga tgcgatatcg
    10141 ggcagcgcca taaaagatga cgatgcattt agcgattcac aggcggatcg cggtctatac
    10201 cttaaaggat atgccgaagg acaaaagaaa acttgccaga ccgattttac ttatgcccga
    10261 ggactttccg gtaaaagctt tcctgccagc tgcaacaatg ttgaaaatgc cagccaactg
    10321 catgaagtat ggcaaaaagg ggctgatgaa aatgccagca caatacgatt aaattaatta
    10381 aaaccccaca aataaattaa acataagatt ttactcattg tgttttattt ctcacattga
    10441 tgacggtcgc atgaaaaaat gatattttaa tgagcattct gaaaaaatat cgatgcattt
    10501 cgagcgaaga tggtggggat ccctgaatgc gtttttctca ccgactgttc ttacttctga
    10561 ttctcctgct gacgggagca ccaatactcg ctctggagcc ttctgacgtt gcgaaaaacg
    10621 tgcgtatgat ggtttccggt attgtcagtt acacccgctg gcctgcgcta tcagggccac
    10681 caaagttatg tatcttttca tcttcacgtt ttagtaccgc cctacaagag aatgccgcga
    10741 catctttgcc ttatctgcct gtcatcattc acactcaaca agaggcgatg atttcaggct
    10801 gcaatggttt ttattttggc aacgagtcac cgacattcca gatggaatta accgaacaat
    10861 atccgtcaaa ggcgttatta ttaattgccg aacaaaatac cgagtgcatt attggtagcg
    10921 ccttttgtct gatcatccac aataatgacg tcagatttgc cgtaaacctg gatgccttat
    10981 cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct cgcacggaag aaaaatgatg
    11041 gataacgata attctcttaa taagcgcccc acgtttaaaa gagcattacg caacatcagt
    11101 atgaccagca tatttatcac tatgatgctg atctggttgc tgctttccgt gacctcggtg
    11161 ctgaccctga aacagtacgc gcaaaaaaac ctggcactga cagcagcaac aatgacttac
    11221 agtctggaag cagctgtcgt ttttgccgat ggccctgcag caactgaaac actggcagcg
    11281 ctgggccagc aagggcaatt ttcaactgca gaagtacgtg ataagcagca aaatattctg
    11341 gcatcctggc attacacccg taaggatcca ggcgatactt tcagtaattt cataagccac
    11401 tggctcttcc ctgcccccat cattcagccg attcgtcaca atggtgaaac cattggcgaa
    11461 gtacgcttaa ccgctcgcga cagttcaatc agccatttta tctggttttc gctcgccgta
    11521 ctgaccggtt gtattctgct ggcatcaggc atcgcaatta ccctcacccg ccatttgcac
    11581 aatggcctgg tagaagcgct gaaaaatatc accgatgtcg tacatgatgt gcgttccaac
    11641 cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc
    11701 gacttcaaca gtctgctgga tgaaatggaa gagtggcagc ttcgtttaca ggctaaaaat
    11761 gcgcagcttc tacgtaccgc gctacatgac ccattaaccg ggctggctaa ccgcgcagcg
    11821 tttcgtagcg gcatcaacac gttgatgaac aattccgatg cccgaaaaac gtcggcgtta
    11881 ctatttcttg atggcgataa tttcaaatac atcaatgata cctggggtca tgcgacgggc
    11941 gatagagtct tgattgaaat cgcaaaacgg ttagctgaat ttggcgggct gcgacataaa
    12001 gcataccgcc tgggcggcga tgaattcgct atggtgctct atgatgtaca gtcggaatct
    12061 gaagtgcagc agatatgttc agcactgaca caaatcttta atctcccgtt tgatcttcat
    12121 aatggccatc agaccaccat gacattaagc attggttacg cgatgaccat tgagcacgcc
    12181 tctgcggaaa aattacaaga gcttgccgat cacaatatgt atcaggccaa acaccagcgt
    12241 gccgaaaagc tggtgagata acaaggatat atcgatatga taaagcacct ggtagcaccc
    12301 ctggttttca cctcactaat actgactggc tgccagtccc ctcagggaaa gtttactcct
    12361 gagcaagtcg ccgctatgca atcttatgga tttactgaat ccgccggcga ctggtcgctg
    12421 ggcttatcag atgccattct gttcgcaaaa aatgactaca aattgctccc ggaaagccag
    12481 caacagatcc aaaccatggc agctaaattg gcctcgacag ggctaacaca tgcccgtatg
    12541 gatggacaca ccgataacta tggtgaagac agttacaacg aaggcttatc attgaaacgg
    12601 gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa ttccacgcag caatctcacc
    12661 acacagggtt taggaaaaaa atatcccata gccagtaaca agaccgccca gggccgcgcc
    12721 gagaaccgcc gcgtcgcagt ggtgattact accccttaaa acaccagcaa acagataaaa
    12781 aagggccagc caattggcca gcccttctta acaggatgtc gcttaagcga aatcttagtt
    12841 aagacgctct ttgatacgag cagccttacc agtacgctca cgcaggtagt acagtttagc
    12901 tttacgaaca gcaccacgac gtttgacaga aatgctgtca actaccggag agtgagtctg
    12961 gaagacacgc tcaacgcctt cgccgttgga aattttacga acagtgaatg cagagtgcag
    13021 accgcggtta cgaatagcga taaccacgcc ctcgaatgcc tgcagacgtt ttttggaacc
    13081 ttcaacaacc catactttca cttccacggt atcacccgga cggaaggaag gtacgtcctg
    13141 cttcatctgc tcttgttcaa gttgcttaat aatgttgctc ataatttaat ctcttatcct
    13201 gggtaaactg atatctcggg ggcttacgcc atcccatcat gtttatgttg ctgttgtgcg
    13261 tgttccgttt tgaactccgc cagcaacctt gcttgctctt cagtcagagc caggttttcc
    13321 agaagttcag gtcttctaag ccaggtacgg cccagcgact gtttcaaacg ccagcgacgt
    13381 atctcggcat ggttgcccga cagtaacact ggcggaactt ccatcccttc taacacctca
    13441 ggccgcgtat agtgcgggca atccagcaat ccttcagcaa aggaatcttc cgttgccgag
    13501 gcttcatgtc ccagtactcc cggaataaac cgggaaacgg agtcaatcag cgtcattgct
    13561 ggtaactcac caccactgag aacgtaatcg ccgattgacc attcttcgtc aatttcggtt
    13621 tggatcacgc gctcatctat accttcgtag cgaccgcaca ccagaatcaa tttttgattc
    13681 gttgccagtt cgctgacgcc cgcttgatca agcttgcgtc cctgtggtga cagataaatc
    13741 acctttgcgc cttcacccgc cgcggctttt gctgcatgaa tggcgtcccg caagggttgc
    13801 accatcatta acatccccgg tccgccgccg taaggacgat cgtccacggt acggtgccgg
    13861 tcatgcgtga agtcgcgagg actccagctc tggatgctca gcaggccatt tttaactgcc
    13921 cggccagtta ccccgtaatc ggtaattgcg cggaacattt caggaaacag gctaattatg
    13981 ccaatccaca tagcgccgtc ttttaccgtt tatccggtgg tttaaaaacc aggatcccaa
    14041 tctacttcga ttgaacgagt agtgagatcg actttcttga taacctgccc atcgaggaac
    14101 ggtacgagac gttccttgat accaaacgca tctttcaggt ttgccttaat gacgagaacg
    14161 tcattagatc cggtttccat catatcgacg actttaccga gatcgtagcc ttcagtggtt
    14221 actacctggc agcccatcag gtctttccag tagtagtccc cctcttcaag ctgaggcagc
    14281 tgcgatgaat ccacgacaat ttcacaattc gtcagcaggt tcgccgcatc acgatcgtta
    14341 acgcctttca gcttgatgat catgtcctga ttatggtgct tccagctttc cagctggact
    14401 tgctgccact gacccgcctt ctggataaac cagggctgat agtcaaaaat gctttcggcg
    14461 tcttcggtgg aagaaaacac tctgagccac ccacgaatac cgtaagacga acccattttt
    14521 cccaaaacga tgggatcaac aggtgcttgc gcggtgagtt gtttgctcat catgaccacc
    14581 gtgacagatt aagctgcttt gtttacttct ttgatcagcg cagcaacgcg atcagaaata
    14641 gttgcgccct ggccaaccca gtgagcgatg cgatccagat ccaggcgagt gccttcttct
    14701 ttttcgctag cgattgggtt gaagaaacca acgcgctcga tgaagcgacc gttgcgtgca
    14761 ttacggctgt cagcgacaac aacctggtag aacggacgct ttttagcgcc gtgacgtgct
    14821 aaacgaatag ttaccataac atcctcttgt gtgaataaaa caaccggacc ccatcgagga
    14881 acggagtccg gtgtcatatt aaaagcccga aaattttact catttttgcg ggaattgcaa
    14941 tcaacagttg ctaactctgc tgtaaaaggc cgtcggcggt gcagccagtt tggtgccgga
    15001 gtgcgcgcag tcaccggagc gtacacgcag tacgtgagga tgacgagcac atcccggtgc
    15061 caaaatggca aacaagccag gccgattagc gaccagggaa gcctgggggc atcataccct
    15121 tcatgcttct catcatcttc gccattccgc ccttcttcat tttcttcatc atgcgctgca
    15181 tgtcgtcgaa ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag
    15241 caatacggcg tttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca
    15301 tcgagttgat gatggcttcc atacgcacca gcactttatc gtccatctgt gacttgacgt
    15361 tatccgggat ctgccccatg cccggcagct tgcccatcag actagccatg ccgcccatat
    15421 ttttcatctg gcgcagctgc tcaagaaagt cgttgagatc gaagccgtca ccttttttca
    15481 gcttgctggc taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca
    15541 gcgacagtac gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga tggaacggct
    15601 ccagcgcctc agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac
    15661 gaatagagag cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg
    15721 taagcggtaa cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg
    15781 cgtcaaccac aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt
    15841 ccatcatcgc ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga
    15901 atttcagttt ggcttctttc agcgccgcgt taacgatatc taccggcttc tgaccaacat
    15961 cagaagggaa gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg
    16021 ccgggcgata aacgtcggca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga
    16081 acttaccgag tttaccaacg ctggttgttt taccggcacc ttgcaggccc gccatcagta
    16141 cgaccgcagg cggttgcgca gccaggttca gggtctggtt ctcttcgccc atcgccgcaa
    16201 ccagttcgtt acggactatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt
    16261 catgaccaac cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcta
    16321 cgtccgcctc cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag
    16381 tgaggcgtcc acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat
    16441 caaacattgt ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt
    16501 attttgccgc agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt
    16561 cacctgacgc tatactgctt ctctttctta ttgctcaaac tgtcgacatc actatgcccg
    16621 tttttgctct gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc
    16681 tgctgcaaaa aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg
    16741 tctgccacgc aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc
    16801 tcagcctgct gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg
    16861 tggcttctcg caatcgtggc tggctgctgc tacccattgt ctatgccttt gcgcttatca
    16921 acctggcgct ggcaaccttc atgcccaatg aatacatcac ccatctggaa gctacgcctg
    16981 ggatgctggt gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc
    17041 tgtacgcgct gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcgttta
    17101 accaggaaat gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg
    17161 gcgtggtgct gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacttattta
    17221 gcatggaaaa tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc
    17281 tgctgtgggg acattatcat gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg
    17341 cgggcgcggt aattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca
    17401 gctaaaccca gaaaaggagt ttcccctgga acacatttct actactacgt tgatcattat
    17461 tctgatcatc atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac
    17521 cctcaaccgc tatcgtctgc gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt
    17581 cgaaaaattg ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg gcaataacct
    17641 ggtcaatatt cttgcctccg cgctcggcac tattgttggg atgcgtttgt acggcgatgc
    17701 gggcgtggca attgcgactg gtgtgctgac ttttgtcgta ctggtatttg ctgaggtatt
    17761 gccgaaaacc attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct
    17821 ggctccgctg caaattttga tgatgccgct ggtctggttg ctgaatgcta tcacccgtat
    17881 gctgatgcgc atgatgggta tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga
    17941 agagttgcgc actatcgtgc acgaatcacg ctcacaaatt tcccgtcgca atcaggatat
    18001 gctgctgtcg gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg tgccgcgcag
    18061 tgaaattatc ggtattgata tcaacgatga ctggaaatcg attctgcgcc aactctccca
    18121 ctcacctcac gggcgcatcg tgctctaccg tgattcgctg gacgacgcca tcagtatgct
    18181 gcgagtacgt gaagcctggc ggttgatgtc ggagaaaaaa gagttcacca aagaaaccat
    18241 gctgcgcgcc gcggacgaga tctattttgt gccggaaggt acgccgctca gcacgcagtt
    18301 ggtaaagttt cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat
    18361 tcaggggctg gtgacggttg aagatattct ggaagagatt gtcggcgatt tcactacgtc
    18421 gatgtcgcca acacttgccg aagaggtcac gccgcaaaac gacggttcgg tgattatcga
    18481 tggcaccgcc aacgtgcggg aaatcaacaa agcctttaac tggcatctac cggaagatga
    18541 tgcccgcacg gttaatggcg tcattcttga ggcactggaa gagatccctg tcgcaggcac
    18601 ccgcgtgcgt attggcgagt acgatatcga tattctcgac gtacaggaca atatgattaa
    18661 gcaggtaaaa gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg
    18721 gcccggcatt cgcatgcagg gccgtgaatt attacgaaag cagaaattaa gcttttgctt
    18781 tcgctacagt aaccatcgcc gcacgaatcg tacgaccatt cagcgtataa cccttctgca
    18841 taatgcccag tacgttacct ggcgcaacgt catcagattc caccattgcg atggcctgat
    18901 gcacattcgg gtccagtggg acgttagttt cggcgatcac ttcaacgcca aacttacgca
    18961 caacatccag catcgacttc agcgtcagct caatgccttc aaccatcgca gacatatccg
    19021 ggttagcttt atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt
    19081 cgttgatgaa tttctccagc gcgaatttgt gggctttttc aatatccagt tcagtacgac
    19141 gacgcaggtt ttccatttcg gcttttacac gcaaaatgcc gtcacgttca cgggtctggg
    19201 cttcagccag ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag
    19261 cagaagcttc tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg
    19321 gggcttgccc ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt
    19381 cgcattcatc tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc
    19441 actcacattg tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag
    19501 tgtattggca ttgtgggaca cccacggcac cccactgcac tgacaacaca tgaaatgctc
    19561 taccgctggc tgtgcacaaa aggttacgag gtcatcgttg agcaacaaat cgctcacgaa
    19621 ctgcaactga agaatgtgaa aactggcacg ctcgcggaga ttgggcaact agctgatctc
    19681 gcggtagtcg ttggtggcga cggtaatatg ctgggcgcgg cacgcacact cgcccgttac
    19741 gatattaaag ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc
    19801 gataacgccc agcaacagtt agccgatgtg ctggaaggcc actacatcag cgagaaacgt
    19861 tttttgctgg aagcgcaagt ctgtcagcaa gattgccaga aacgcatcag caccgcgata
    19921 aatgaagtgg tgcttcatcc aggcaaagtg gcgcatatga ttgagttcga agtgtatatc
    19981 gacgagatct ttgcgttttc tcagcgatct gatggactaa ttatttcgac gccaacaggc
    20041 tccaccgcct attccctctc tgcaggcggt cctattctga ccccctctct ggatgcgatt
    20101 accctggtgc ccatgttccc gcatacgttg tcagcacgac cactggtcat aaacagcagc
    20161 agcacgatcc gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc
    20221 cagatagcac tgccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat
    20281 ctgaatctga ttcatccgaa agattacagt tatttcaaca cattaagcac caagctcggc
    20341 tggtcaaaaa aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta
    20401 tactgtacac aataacagta atggtttttc atacaggaaa acgactatgt tggcacaact
    20461 gaccatcagc aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac
    20521 cgtaataact ggcgagaccg gcgcgggtaa atctattgca atagatgccc tcggtctttg
    20581 tctcggtggt cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg
    20641 cgcccgtttt tctctgaaag atacgccagc ggctctgcgc tggctggaag aaaaccagct
    20701 tgaagacggg catgaatgtt tgcttcgtcg cgtgatcagc agcgatggtc gctcccgtgg
    20761 tttcatcaac ggtacagctg ttcctctgtc acaactgcgc gaactgggtc agttgctgat
    20821 tcagatccat ggtcagcacg ctcatcaatt actcaccaaa cctgagcacc aaaaattcct
    20881 gcttgatggc tatgccaatg aaacctctct actgcaggaa atgaccgcac gttatcagtt
    20941 gtggcatcaa agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgccgc
    21001 ccgtgcggaa ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg
    21061 agagtttgaa caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac
    21121 caccagccag aatgcattgg cattaatggc cgacggtgaa gacgcaaacc tgcaaagtca
    21181 gctttacacg gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg
    21241 cgtacttgat atgctggaag aagctaccat ccagattgct gaagccagcg atgaactgcg
    21301 ccactactgc gatcgtctgg atctcgatcc caaccgacta tttgaacttg aacagcgcat
    21361 ctcaaaacag atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat tgccacagta
    21421 ttaccagtcg ctactggaag aacagcagca actggacgat caggccgact cacaagaaac
    21481 gcttgcgctg gcggtaacga aacatcatca gcaggcactg gaaatcgcgc gcgcattaca
    21541 ccaacaacgc cagcaatatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc
    21601 gctctcaatg ccgcatgggc agtttacgat cgatgttaaa tttgacgagc atcacctggg
    21661 cgctgacggt gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca
    21721 gcctattgcc aaagtcgcat ccggtggtga attgtcccgc atcgcactgg caatccaggt
    21781 catcacggcg cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat
    21841 tagcggtcca acagcggcag ttgtcggcaa actgctgcgt caactcggcg aatcaactca
    21901 ggtgatgtgt gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt
    21961 cagcaaagaa accgatggtg cgatgacaga aacgcatatg caatccctga ataaaaaagc
    22021 gcggttacaa gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta atacactggc
    22081 gaatgcgaaa gaactgcttg cagcgtaaac ttttttcctg cttcacggtc agagtaaaca
    22141 gcaaaacgcc gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat
    22201 cggcatatta cagatgagcc acgtactgct cgggcccgaa aaggaatcaa atcactatgc
    22261 gctgtaaaac gctgactgct gcagcagcag tactattgat gttgaccgca ggctgttcca
    22321 ctctggagcg agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg
    22381 acgtatccaa aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac
    22441 cgctgatgtc cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag
    22501 gtcatgaagg tgtaactcag caaacgctga cgctgacctt taacagtagc ggtgtgttga
    22561 ccaatattga taacaaacct gcgctgagtg gtaactaata ataaagttgc tctcaaagac
    22621 gttaaaaaag gtgctcaatg agcacctttt ttctgtctgt tatttattcg ctgatttttc
    22681 tgctcgttgc ctgcgaagct ctttcggatc ggcaatgaga gggcgataaa tctccacccg
    22741 atcgccatca tgcacactat cgcttagttt tgccggacgg ctgtaaatgc cgactttgtt
    22801 tttagttaaa tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc
    22861 aaccgtcgcg ccctcctgca gcgtcactcg ctgcaggtac tgcttctcag gtagcgcata
    22921 agccacctca acggcaattt tacctggcac tgtaaacctc tttcgcacga accgtaaaag
    22981 cctggaccat attagccgcc agctctttaa acacgcgacc aaaggcgagt tcaatcaact
    23041 tattggtaaa ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg
    23101 taaacttcca tccaccaatc aatttcttga acggcccatc caccagattc ataagaatac
    23161 tttggttact ggtcaactgg ttgcgggtag taaacgtttt gctgatccca gccttagaga
    23221 catctaccgc cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac
    23281 ccggcaaaaa ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc
    23341 tgtagggtac cagtgcggtc cggctaatct gaggcataac aatttccatc aacaaaaatc
    23401 caacaaataa tatcatttat cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat
    23461 cgggttcatg ctaagataga accttgtccc ccgcaggatt gatatggggt gttttcgatt
    23521 tcagattacc gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca
    23581 gcgaccatcg cgcttaacaa gcgcgcccgt cacgaatact ttatcgaaga agagttcgaa
    23641 gcgggacttg ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa agccaatatc
    23701 agcgacagct acgtccttct gcgtgacgga gaggcatttc tgtttggcgc taacatcacg
    23761 ccaatggccg tggcctccac gcatgtggtg tgcgatccta cccgtacccg caagttactt
    23821 ctcaaccagc gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg ctataccgta
    23881 gtggcgctct ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa
    23941 ggtaagaaac agcacgataa acgttcagat atcaaagagc gcgaatggca ggtggataaa
    24001 gcacgtatca tgaaaaacgc ccaccgttaa acctgcactc caattattga ccagttcctc
    24061 accgcgcctc cctctccggc ggcgcgaatg aacatcttat tggctatcac atccgacaca
    24121 aatgttgcca tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt
    24181 aacgataacg ccattgaggc tggtcatggc gctcataaat ctggtatact tacctttaca
    24241 cattggggct gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc
    24301 ggttggcctc gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca
    24361 gcttaataac ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag
    24421 agaggtcaaa cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact
    24481 taatcaggct agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact
    24541 gactaagcat gtagtaccga ggatgtagga atttcggacg cgggttcaac tcccgccagc
    24601 tccaccattt ttgacaggac tgcaaccgga catcgacaat aaaaacagat aattacggac
    24661 actgaccaga caacaaacag acctgaacaa gacaaaaata tacacgtgaa atgcacgtgc
    24721 actttataaa aaccccagat ttcacagtct ggggtttttc tgtttgtaaa taatggtaac
    24781 aaaaacatat caccttttgg cgttccgttc ctcttgacac tgtttatcca tacagttgaa
    24841 aataatactg tatacaaaca cagtgtagag ggatttttat gcgtattgaa atctgtatcg
    24901 ctaaagaaaa attcagcaaa atgccaaaag gctctgtacc tgctctacaa caggaaatgt
    24961 ctcgccgtat cagaaaac
//
LOCUS       DBBGLQ010000061        24551 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.061, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000061 DBBGLQ010000000
VERSION     DBBGLQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.061"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..222
                     /locus_tag="ACIYSB_003401"
     CDS             <1..222
                     /locus_tag="ACIYSB_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGC5430380.1"
                     /translation="DLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELL
                     LEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            378..1913
                     /gene="gadC"
                     /locus_tag="ACIYSB_003402"
     CDS             378..1913
                     /gene="gadC"
                     /locus_tag="ACIYSB_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGC5430381.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2044..3363
                     /locus_tag="ACIYSB_003403"
     CDS             2044..3363
                     /locus_tag="ACIYSB_003403"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGC5430382.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3727..5109
                     /gene="dosC"
                     /locus_tag="ACIYSB_003404"
     CDS             3727..5109
                     /gene="dosC"
                     /locus_tag="ACIYSB_003404"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGC5430383.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5134..7533
                     /gene="dosP"
                     /locus_tag="ACIYSB_003405"
                     /gene_synonym="pdeO"
     CDS             5134..7533
                     /gene="dosP"
                     /locus_tag="ACIYSB_003405"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HGC5430384.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEIQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDLGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7791..8372
                     /gene="ddpX"
                     /locus_tag="ACIYSB_003406"
     CDS             7791..8372
                     /gene="ddpX"
                     /locus_tag="ACIYSB_003406"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGC5430385.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8386..9936
                     /locus_tag="ACIYSB_003407"
     CDS             8386..9936
                     /locus_tag="ACIYSB_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGC5430386.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGIIVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9938..10960
                     /locus_tag="ACIYSB_003408"
     CDS             9938..10960
                     /locus_tag="ACIYSB_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5430387.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10957..11853
                     /gene="ddpC"
                     /locus_tag="ACIYSB_003409"
     CDS             10957..11853
                     /gene="ddpC"
                     /locus_tag="ACIYSB_003409"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGC5430388.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTSAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11850..12836
                     /locus_tag="ACIYSB_003410"
     CDS             11850..12836
                     /locus_tag="ACIYSB_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5430389.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIDLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12829..13755
                     /locus_tag="ACIYSB_003411"
     CDS             12829..13755
                     /locus_tag="ACIYSB_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGC5430390.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(13811..14242)
                     /gene="osmC"
                     /locus_tag="ACIYSB_003412"
     CDS             complement(13811..14242)
                     /gene="osmC"
                     /locus_tag="ACIYSB_003412"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGC5430391.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14587..14802
                     /gene="bdm"
                     /locus_tag="ACIYSB_003413"
     CDS             14587..14802
                     /gene="bdm"
                     /locus_tag="ACIYSB_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGC5430392.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14904..15041
                     /gene="sra"
                     /locus_tag="ACIYSB_003414"
     CDS             14904..15041
                     /gene="sra"
                     /locus_tag="ACIYSB_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGC5430393.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15198..16895
                     /gene="maeA"
                     /locus_tag="ACIYSB_003415"
     CDS             15198..16895
                     /gene="maeA"
                     /locus_tag="ACIYSB_003415"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGC5430394.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGMV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17029..18039
                     /gene="adhP"
                     /locus_tag="ACIYSB_003416"
     CDS             17029..18039
                     /gene="adhP"
                     /locus_tag="ACIYSB_003416"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGC5430395.1"
                     /translation="MKAAVVTKDHHVDVTYKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKIGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSHTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            18185..18469
                     /locus_tag="ACIYSB_003417"
     CDS             18185..18469
                     /locus_tag="ACIYSB_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGC5430396.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18698..19351)
                     /gene="fdnI"
                     /locus_tag="ACIYSB_003418"
     CDS             complement(18698..19351)
                     /gene="fdnI"
                     /locus_tag="ACIYSB_003418"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGC5430397.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19344..20228)
                     /gene="fdnH"
                     /locus_tag="ACIYSB_003419"
     CDS             complement(19344..20228)
                     /gene="fdnH"
                     /locus_tag="ACIYSB_003419"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGC5430398.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20241..23288)
                     /gene="fdnG"
                     /locus_tag="ACIYSB_003420"
     CDS             complement(20241..23288)
                     /gene="fdnG"
                     /locus_tag="ACIYSB_003420"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22701..22703),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGC5430399.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQSEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23520..24401
                     /gene="yddG"
                     /locus_tag="ACIYSB_003421"
     CDS             23520..24401
                     /gene="yddG"
                     /locus_tag="ACIYSB_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGC5430400.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATHHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMIFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6015 a         6219 c         6188 g         6129 t
ORIGIN      
        1 gacctctctg aacgtctgcg tctgcgcggc tggcaggttc cggccttcac tctcggcggt
       61 gaagccaccg acatcgtggt gatgcgcatt atgtgtcgtc gcggcttcga aatggacttt
      121 gctgaactgt tgctggaaga ctacaaagcc tccctgaaat atctcagcga tcacccgaaa
      181 ctgcagggta ttgcccaaca gaacagcttt aaacatacct gataacgttt aacggtaacg
      241 gtgtcccgaa acgaacccgt ttcgggacaa tttccaaagt ctgttcactg gcattagcaa
      301 cggaaaatat tgttctgaat acgcttcaga acaaaacagg tgcggttccg acaggaatac
      361 cgttttaggg ggataatatg gctacatcag tacagacagg taaagctaag cagctcacat
      421 tacttggatt ctttgccata acggcatcga tggtaatggc tgtttatgaa taccctacct
      481 tcgcaacatc gggcttttca ttagtcttct tcctgctatt aggcgggatt ttatggttta
      541 ttcccgtggg actttgtgct gcggaaatgg ccaccgtcga cggctgggaa gaaggtggtg
      601 tcttcgcctg ggtatcaaat actctggggc cgagatgggg atttgcagcg atctcatttg
      661 gctatctgca aatcgccatt ggttttattc cgatgctcta tttcgtgtta ggggcactct
      721 cctacatcct gaaatggcca gcgctgaatg aagaccccat taccaaaact attgcagcac
      781 tcatcattct ttgggcgctg gcattaacgc agtttggtgg cacgaaatac acggcgcgaa
      841 ttgctaaagt tggcttcttc gccggtatcc tgttacctgc atttattttg atcgcattag
      901 cggctattta tctgcactcc ggtgcccccg ttgctatcga aatggattcg aagaccttct
      961 tccctgactt ctctaaagtg ggcaccctgg tagtatttgt tgccttcatt ttgagttata
     1021 tgggcgtaga agcatccgca acccacgtca atgaaatgag caacccaggg cgcgactatc
     1081 cgttggctat gttactgctg atggtggcgg caatctgctt aagctctgtt ggtggtttgt
     1141 ctattgcgat ggtcattccg ggtaatgaaa tcaacctctc cgcaggggta atgcaaacct
     1201 ttaccgttct gatgtcccat gtggcaccag aaattgagtg gacggttcgc gtgatctccg
     1261 cactgctgtt gctgggtgtt ctggcggaaa tcgcctcctg gattgttggt ccttctcgcg
     1321 ggatgtatgt cacagcgcag aaaaacctgc tgccagcggc attcgctaaa atgaacaaaa
     1381 atggcgtacc ggtaacgctg gtcatttcgc agctggtgat tacgtctatc gcgttgatca
     1441 tcctcaccaa taccggtggc ggtaacaaca tgtccttcct gatcgcactg gcgctgacgg
     1501 tggtgattta tctgtgtgct tatttcatgc tgtttattgg ctacattgtg ttggttctta
     1561 aacatcctga cttaaaacgc acatttaata tccctggtgg taaaggggtg aaactggtcg
     1621 tggcaattgt cggtctgctg acttcaatta tggcgtttat tgtttccttc ctgccgccgg
     1681 ataacatcca gggtgattct accgatatgt atgttgaatt actggttgtt agtttcctgg
     1741 tggtacttgc cctgcccttt attctctatg ctgttcatga tcgtaaaggc aaagcaaata
     1801 ccggcgtcac tctggagcca atcaacagtc agaacgcacc aaaaggtcac ttcttcctgc
     1861 acccgcgtgc acgttcacca cactatattg tgatgaatga caagaaacac taaccatgtc
     1921 tgaatggtta taagacaagg gagcgataat tcatcgctcc ctttttcgtg cttgctgtcg
     1981 tattgactta acggggataa gtacgagaat gagcgcacat ctgtttaccg gaaaccagca
     2041 catatggata tctgctcccg aaacaagaaa ttaacgatta gaagaccagc gatactagtt
     2101 gcactggcac ttttactgtg tagttgtaaa agcacgcctc cagagtccat ggtgacacca
     2161 ccagcaggtt caaagccacc agccacgacg caacaatcgt cacaaccgat gcgtggcatc
     2221 tggctggcca cggtttctcg gctcgactgg ccaccggttt cctcggttaa cattagtaac
     2281 cccaccagcc gggcccgtgt acaacaacag gcgatgatcg acaaactgga tcatctgcaa
     2341 cgtctcggca taaacacggt ctttttccag gtcaagccgg acggtaccgc cctgtggcca
     2401 tcgaaaattt tgccgtggtc cgatcttatg accggtaaga ttggtgaaaa tccgggttac
     2461 gatccgctgc aatttatgct cgacgaagcc cacaagcgtg ggatgaaagt acacgcctgg
     2521 tttaacccct atcgcgtatc ggttaatacg aagcccggta ctatcaggga actgaatagc
     2581 actctgtctc aacaaccggc gagcgtctat gtgcaacacc gcgactggat cagaacgtct
     2641 ggcgatcgct ttgtcctcga cccgggcatc cctgaggttc aggactggat cacatcaata
     2701 gtcgcagaag tggtttcccg ctatcccgta gatggcgtgc agtttgacga ctatttctat
     2761 acggagtcac cgggttcacg gctaaatgat aacgaaacgt accgtaaata cggaggcgca
     2821 tttgcgtcaa aagcagactg gcggcgcaac aatactcagc agttaattgc aaaggtatcg
     2881 cacaccatta aaagcattaa gccgggagtc gaatttggtg ttagcccggc aggcgtgtgg
     2941 cgtaaccgat cacacgatcc gctcggttcc gatacccgag gcgcggcagc ctatgacgaa
     3001 tcctacgctg acacccgtcg atgggtggaa caaggattgc tggattacat tgctccccaa
     3061 atttactggc cgttctcacg gagtgccgcg cgttatgacg tgttggcaaa atggtgggcg
     3121 gatgtcgtta aaccgaccag gacccgcctg tatatcggta tcgccttcta taaagtgggt
     3181 gaaccttcaa agatagagcc agactggatg attaacggcg gcgtaccgga actgaaaaag
     3241 cagctcgatc ttaacgatgc tgtgcccgaa attagcggca ccatcttgtt ccgtgaggac
     3301 tatctgaata aaccgcagac tcaacaagcg gtcagctatc tgcaaagtcg ctggggcagt
     3361 taaactccca ttcttacttt gtaacaagcc ggtgctttac ccaccggctt gttaccctct
     3421 taaactatcc cactaataat catgcttact taagtcaaat taaccacact tagttaaaca
     3481 gccataaatc cctaataaaa atgtatgaat atgaaaaacc atacgatatt cgctgtcacc
     3541 gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat tgaaactgtc
     3601 ctaaatcgct tatttctttt cagtatatct tcatatttca ggagaatatg gtgcagttag
     3661 tcgatctcgc acgttgcgtt tcattttgat tccgtctgat ttcccagcct tataagggtg
     3721 tgaattatgg agatgtattt taaaagaatg aaagatgagt ggaccggact tgtcgaacag
     3781 gcagatccgc ccattcgtgc taaagccgcg gaaattgccg ttgcgcatgc tcattatctg
     3841 agtatcgagt tttatcgaat tgtccgcatc gacccgcatg ccgaagaatt cttgagtaat
     3901 gaacaagttg agcggcagtt gaagagtgcg atggaacgct ggattattaa cgtgctttct
     3961 gcccaggttg acgatgtcga aaggctaata caaatccagc ataccgtcgc ggaagtgcat
     4021 gcccgcatag gaattccggt agaaattgtc gagatggggt ttcgggtgct gaaaaaaatc
     4081 ctctatccgg tgatcttctc ttcggattat tccgccgcag aaaaacttca ggtctaccat
     4141 ttctcgatta acagtattga tatcgcgatg gaagtgatga ctcgcgcgtt tacctttagt
     4201 gacagtagtg cctcaaagga agatgaaaac tatcgtatct tctcgttact ggaaaacgcc
     4261 gaagaagaaa aagaacggca aatagcctca atactttcat gggaaataga tattatctat
     4321 aaaatcctgc tggattctga tttaggcagt agtttgcctt taagccaggc tgattttggc
     4381 ctgtggttta accataaagg tcgacattat tttagtggta ttgccgaagt aggccatatc
     4441 tcccgtctga ttcaggattt cgacggtatt ttcaatcaaa ccatgcgtaa caccagaaat
     4501 ttgaataaca gaagtctgcg ggtgaaattt ttattacaga taagaaatac cgtatcgcaa
     4561 attattacct tgctgcgtga attgtttgaa gaagtatcgc gccacgaagt cggtatggat
     4621 gtactgacga aattacttaa ccgccgtttc ctaccgacta tcttcaaacg cgaaattgcc
     4681 catgccaacc ggaccggtac accgctgtca gtgctgatta ttgacgttga taaattcaaa
     4741 gagatcaacg atacgtgggg ccataacact ggtgatgaaa ttctgcgtaa agtctctcag
     4801 gccttttatg acaacgtccg cagtagtgat tatgttttcc gctacggggg cgatgaattt
     4861 atcattgttt tgactgaagc ttctgaaaac gaaacgttac gtaccgcaga acgtattcgc
     4921 agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg aagatattgc cctctcactt
     4981 tccatcggtg ccgccatgtt taatggtcat cctgactatg agcgcctcat tcaaatagcc
     5041 gatgaagctc tgtatatcgc caaaagacga ggtagaaacc gtgttgaact ctggaaagcc
     5101 agtctttaga tgcgccagga tgcagaggta atcatgaagc taaccgatgc ggataatgcc
     5161 gccgatggca tttttttccc cgcccttgag caaaatatga tgggtgcggt gttaattaac
     5221 gaaaatgatg aagtgatgtt tttcaacccc gccgcagaga agctctgggg atacaaacgt
     5281 gaagaagtca ttggcaataa cattgatatg ctgattccgc gggatttgcg tcctgcgcat
     5341 cctgaataca ttcgtcacaa ccgtgaaggc ggtaaagcgc gtgttgaggg gatgagtcgg
     5401 gagctgcagc tggagaaaaa agacggcagt aaaatctgga cccgttttgc gctatcgaaa
     5461 gtgagcgccg aggggaaagt ttattacctg gcgctggtac gggatgccag cgtagaaatg
     5521 gcgcaaaaag aacagacccg acaattgatt attgccgttg accatctcga ccgaccggtg
     5581 attgtcctcg atccggaacg ccatattgtg cagtgcaatc gcgcatttac cgaaatgttt
     5641 ggttactgca ttagcgaagc cagcggtatg cagcccgata cactcctgaa cattcctgaa
     5701 ttccctgccg ataaccgcat tcgtttacaa cagttgctat ggaaaaccgc ccgcgatcag
     5761 gacgaatttc tgctgttgac gcgcaccggt gaaaaaatct ggattaaagc ctctatcagc
     5821 ccggtttatg acgtgctcgc gcatctgcag aacctggtaa tgactttctc ggatatcacc
     5881 gaagaacggc agattcgcca gcttgaaggc aatattctcg ccgccatgtg cagcagcccg
     5941 ccatttcatg aaatggggga aatcatttgt cgtaacatcg aatctgtact caacgaatcg
     6001 catgtttcgc tgttcgcact gcgcaacggg atgccgatac actgggcgtc atcttcccac
     6061 ggtgcagaaa ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg tgatggcgcg
     6121 cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc ctttatcgaa
     6181 cgcgtggcag atatcagcca gcatatggcc gcgctggcgc tggaacagga aaaaagccgt
     6241 cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa tcgcaataac
     6301 ctgcacaatt acctcgatga cctggtcgac aaagccgtct ctcccgtggt gtatctcatc
     6361 ggtgttgacc atattcagga tgtgattgat agccttggct atgcgtgggc cgatcaggca
     6421 ttgctggaag tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta tctctgtcgt
     6481 atcgaaggta cgcagtttgt cctcgtgagc ctcgaaaacg acgtcagtaa cattacccaa
     6541 atcgccgatg agctacggaa tgtggtcagc aagccgataa tgattgacga taaacccttc
     6601 ccgcttacct tgagtattgg catcagctac gacctgggta aaaaccgcga ttacttgctc
     6661 tccactgctc acaatgcaat ggattatatt cgcaagaatg gcggtaacgg ctggcagttc
     6721 ttcagcccgg cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc agcgctgaaa
     6781 gaagcgatta gcaataacca actgaaactg gtttaccagc cgcaaatctt cgcagaaacg
     6841 ggtgaactgt acggcatcga agcccttgct cgctggcacg atcccctgca tggtcatgtg
     6901 cccccttcac ggtttattcc tctcgcagaa gagattggtg aaatcgaaaa tattgggcgc
     6961 tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc gtagccagaa tattcatatc
     7021 ccggcgttat ccgtgaactt gtcggcgctg cactttcgca gtaatcaact gcctaatcag
     7081 gtgtctgatg caatgcacgc ctggggtatt gacggccacc agctgacggt agaaatcacg
     7141 gaaagcatga tgatggaaca cgataccgaa atctttaagc gcattcagat cctgcgtgat
     7201 atgggcgtgg gcttatcggt agatgatttt ggtacgggct tttccggatt atcccgctta
     7261 gtcagtcttc cggtaacgga aatcaaaatt gacaaaagtt ttgtcgatcg ttgtctgacc
     7321 gaaaaacgca tccttgcctt acttgaagcc attaccagca ttgggcaaag cctcaattta
     7381 accgtcgtgg cggaaggcgt cgaaaccaaa gagcaatttg agatgctacg caagatccac
     7441 tgtcgcgtta ttcagggata tttcttttcc cgccccctac ccgccgaaga aattccaggc
     7501 tggatgagca gcgtgttacc gctgaaaatc tgacaaattc ctctcgcccg cactcgcggg
     7561 ttttcattta acgtgacact gtcacttaaa tactgtgatt tcagctacga ttccgggaga
     7621 ttccttcctt aacttcccct cttgcaccaa aactgcacta cgctgcacaa atgttgatca
     7681 aaagttaaaa aatataactt taagccagca ttctggcgct tatcccggca tggcatgaga
     7741 tctgcataag cggaaagcgc agcaattttt gtcttataca ggaaccgttt atgtcggata
     7801 ccaccgaact ggttgattta gccgtaatct tccctgatct agagatcgaa ttgaaatacg
     7861 cctgcgctga taacatcaca ggtaaagcta tttatcagca agcgcgttgt ctgttacaca
     7921 aggatgcgat taccgcgctg gcgaaaagta tcagcatcgc ccagctgtca gggttacaac
     7981 tggtgattta cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg caagcctgcc
     8041 cagacccgca atatgttgtt gatgtgacgg tcggttctaa tcacagccgt ggcacggcga
     8101 tcgacctgac gcttcgtgat gagcacggga acatcctcga tatgggcgca gggttcgatg
     8161 aaatgcacga gcgctcccat gcctatcacc cttccgtccc gcccgccgct cagcgcaatc
     8221 ggctgttgct gaatgcgata atgactggtg gcggttttgt cggtatctcc agcgaatggt
     8281 ggcacttcga attaccccag gcagcgagtt accctctgct cgctgatcaa ttctcttgtt
     8341 ttatatcccc tggcacacag cacgtcagtt aatcaggaaa ctgtcatgaa gagatcgata
     8401 tcgtttcgtc ccacattgct cgcgctcgtc cttgccacaa atttcccggt tgcgcacgcc
     8461 gccgtaccaa aagatatgct ggtgattggt aaggccgccg atccacaaac cctcgacccg
     8521 gcggtaacaa tagataataa cgactggaca gtgacctacc cgtcttatca gcggctggtt
     8581 cagtacaaaa cggacggtga taaaggctca accgacgttg aaggcgatct ggcaagtagc
     8641 tggaaagcgt ctgacgatca aaaagagtgg acgttcaccc tgaaagataa tgctaaattt
     8701 gccgatggca cacctgtcac tgccgaagca gtaaaacttt cttttgagcg gctactaaaa
     8761 atcggccagg ggccagcaga agcatttccc aaagatttaa agattgatgc tcccgacgaa
     8821 catacggtga agtttaccct tagccaacca ttcgcaccgt tcctctacac gctggcgaat
     8881 gacggtgcat ccattatcaa tccggcggtc ttaaaggaac atgcagcgga tgatgctcgc
     8941 ggcttcctcg cgcaaaatac cgccggttcc ggaccattta tgctgaaaag ctggcaaaaa
     9001 ggtcagcaat tagttctggt gccaaatccg cattacccag gcaataaacc gaacttcaaa
     9061 cgggtatcgg taaaaattat tggtgaaagt gcctcccgtc gcctgcagct ctcccgtggc
     9121 gacattgaca ttgccgatgc gctgccggtg gatcaactca acgccctgaa gcaggaaaat
     9181 aaagtcaatg tggcagagta tccgtcactg cgcgttacct atctgtatct gaataacagc
     9241 aaagcgcctc ttaatcaggc ggatctgcgt cgggccattt cctggtctac cgattatcag
     9301 ggcatggtta acggcattct gagtggtaac ggaaaacaga tgcgcggccc gattccggaa
     9361 ggcatgtggg gctacgatgc gacggcaatg caatacaacc atgacgaaac gaaagccaaa
     9421 gccgaatggg ataaagtgac gagcaaaccc accagcctga cgtttctcta ctccgataac
     9481 gatccgaact gggagcctat tgctctggcg acacaatcca gtctcaacaa gctgggcatc
     9541 attgtgaagc tggaaaagct ggcgaacgcc accatgcgcg acagagtggg taaaggtgat
     9601 tacgacattg cgattggcaa ctggagtccg gattttgccg acccgtatat gtttatgaat
     9661 tactggtttg agtcagacaa aaaaggtctg ccgggtaacc gctcgttcta tgaaaacagt
     9721 gaggtcgata agttactgcg caatgcgctt gcgaccaccg accagacgca gcgtacccgg
     9781 gactaccagc aggcacagaa aatcgtcatt gatgacgctg cttatgtgta cctgttccag
     9841 aaaaactacc aactggcgat gaacaaagag gtgaaaggct ttgtgttcaa tcccatgctg
     9901 gaacaggtct tcaatatcaa taccatgagt aaataacgtg accttctgga gtattttacg
     9961 ccaacgctgc tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta cgtttattat
    10021 ctcgcatctg atccctggtg atccggcgcg gttactggcg ggtgaccgcg ccagcgatgc
    10081 tatcgtggaa aatattcgcc agcaactggg actggaccag ccactgtatg tacagtttta
    10141 ccgctacgtc agcgacctgt ttcatggtga cctgggaaca tccattcgta ccgggcgtcc
    10201 ggtgctggaa gagttgcgta tatttttccc ggcgacgctg gagctggctt ttggtgccct
    10261 gctgctggca ctcctgattg gcatcccgct gggcatactc tctgcagtct ggcgaaatcg
    10321 ctggctggat catctggtgc gaataatggc cattaccgga atctccacac ctgcgttctg
    10381 gcttgggctg ggcgtcattg tgctgtttta tggtcatctg caaattcttc ccggcggcgg
    10441 aaggcttgat gactggctgg atccaccaac gcacgttacc ggcttttatc tgctcgatgc
    10501 gctgcttgaa ggcaacggtg aagtcttctt caatgcgttg caacatctca tcttaccggc
    10561 attaacgctg gcgttcgttc acctgggaat tgtcgctcgc cagatccgct cagcgatgct
    10621 ggaacaattg agtgaagact acattcgtac cgcccgggcc agcggcttgc ccggctggta
    10681 tatcgtttta tgttatgcgc tacccaatgc gttgatccca tcgattaccg tattgggttt
    10741 ggcgctgggc gatttgttgt atggcgcagt gctcaccgaa accgtttttg cctggcccgg
    10801 aatgggtgca tgggtagtaa catcaataca ggcgctcgac ttcccggcag tgatgggctt
    10861 tgccgtcgtg gtttcatttg cttatgtgct ggtcaacctg gtggtggatt tgctctattt
    10921 gtggattgat ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga ggaaacgtcc
    10981 gccgtacgcc cacaaaaaca aacgcgattt aacggtgcaa aactggtatg gatgctgaaa
    11041 ggcagtccgc tcaccgtgac cagcgcagtc atcattgtat taatgctatt gatgatgatt
    11101 ttttcaccgt ggctggcgac gcatgatccc aacgccattg atttaaccgc ccgccttttg
    11161 ccgccttctg cggcgcactg gtttggcacc gatgaagtgg gacgcgatct gtttagccgc
    11221 gtactggtcg gcagtcagca atcaattctc gccggattag tcgtggttgc cattgcgggt
    11281 atgattggtt cgctactcgg atgtctatcc ggtgtgcttg gtggacgcgc agacgccatc
    11341 atcatgcgca tcatggacat tatgctgtcg attccttcgt tggtactgac aatggcactg
    11401 gctgccgctc tcgggccgag tttgtttaac gccatgctgg ctattgctat tgtgcgaatt
    11461 cccttttatg tgcgcctggc gcgggggcaa gcattagttg tacgccagta tacctatgtt
    11521 caggcggcga aaacctttgg cgcgtctcgt tggcatctga tcaactggca tattttacgt
    11581 aactccctac cgccgctgat cgtgcaggca tcgctggata tcggtagcgc gattttaatg
    11641 gccgccacgt tgggatttat tggcctgggt gctcaacaac cgagtgctga atggggcgcg
    11701 atggtagcaa atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc atttccgggg
    11761 gcagcgattt tgcttaccgc cgtcgggttt aatctctttg gtgatggtat tcgcgatctg
    11821 cttgacccga aagcaggagg aaagcagtca tgacccaacc cgttctggac attcaacaac
    11881 tgcatttgag tttccccggt tttaacggtg atgttcacgc gctcaacaat gtgtccttgc
    11941 agattaaccg cggtgaaatt gtcggtctgg tgggagaatc cggctcaggt aaatcagtca
    12001 ccgcaatgct gattatgcgt ctgctaccga cgggcagtta ttgcgtacat cgggggcaga
    12061 tttccctgct gggagaagat gttcttaacg cgcgggaaaa acagcttcgt cagtggcgcg
    12121 gcgcacgagt ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg acacgtcgaa
    12181 taggtcttca gatgatggac gtgatccgcc atcatcaacc aataagtcgt cgggaagcca
    12241 gagctaaagc gattgacctg ctggaagaga tgcaaatccc ggatgccgtg gaagttatgt
    12301 cgcgctatcc gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt gcgctggcat
    12361 tctcctgcga gccgcaattg attattgccg acgaaccgac tacggcgctg gacgtcacgg
    12421 tgcagttgca ggtactgcgt ttgcttaaac ataaagcccg cgccagtgga actgcggttc
    12481 tgttcatcag ccatgatatg gccgtggtgt cgcaactgtg cgatagcgtt tacgtgatgt
    12541 atgccggaag cgtaattgaa agcggcgtga cggcagacgt tatccatcat ccccggcatc
    12601 cgtataccat tggtttgctg caatgcgcac cagaacatgg agtaccacgc cagctattac
    12661 ccgccattcc agggacggta ccaaacctca cccatttgcc tgacggctgc gcttttcgcg
    12721 atcgttgcta tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca gcgtgtggtg
    12781 acaacaacca gcgctgtgcc tgttggtatc cgcagcagga ggttataagt gtctgacacg
    12841 ttattaacgt tacgcgacgt ccatatcaat ttcccggccc gtaaaaactg gcttggtaaa
    12901 actacggaac atgttcatgc cattaatggt attgatttac agatccgccg tggtgaaacc
    12961 ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct tttaatgggt
    13021 atgctgcaac cgagccacgg gcagtacatc cgttctggct cacaacgcat tatgcagatg
    13081 gtgtttcagg acccgctctc ttcgcttaat ccgcgcttac cggtgtggcg catcatcaca
    13141 gaaccgctct ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt ggcagaggag
    13201 ttggctgtgc aggtgggtat tcgtccggag tatctcgacc gcctgcctca tgcgttctcc
    13261 ggcgggcagc ggcaacgtat cgccattgcc agagcactct cttcgcagcc tgacgtgatt
    13321 gtgcttgatg agccaacctc tgcgctggat atctccgttc aggcgcagat cctcaattta
    13381 ctggtaacgc tacaggaaaa tcacgggctg acctatgtgc tgatttcaca caatgtctcg
    13441 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg
    13501 ggcgacgcgc agcaggtgct gacggcacct gcacatccat acacccgatt attgctggat
    13561 tccctccccg ccattgataa accgctggag gaagaatggg cattacgtaa aacggatctg
    13621 ccgggaaacc gcacgttgcc gcaaggctgt tttttctacg aacgttgccc gctggcaacc
    13681 cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga gctccggtgc
    13741 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    13801 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    13861 agaccgggca tcctgctttt gctttctgga ttatgccgtc aaaggtagag gcatcaatac
    13921 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg gcatccactt
    13981 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca
    14041 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    14101 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    14161 cgccactctc ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    14221 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    14281 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatccgg cgaaaatatt
    14341 acttctgcac ttaaattaaa ttcccacaat agattaccgg gaatatataa cccaaaagat
    14401 attgtatcaa tattaatcgg atcataccaa tcacaatccc tatgaatatc atggtgttaa
    14461 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    14521 tgaatgtgct ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga
    14581 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    14641 gccattgagc aaggccttcg ggcacagcat ggtgttgtca ctgaagatga cattctcatg
    14701 gagctgacca aatgggttga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    14761 acgatcaatt atgtggtcag tggccagcac cctacgcttt aaggtgctat gcttgatcgg
    14821 caacctaatt taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact
    14881 agctacagga ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac
    14941 tggaccataa aatttctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtag
    15001 taaataaccc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    15061 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    15121 tgaggccgac gccctggcgg taaagcaaag acgataaaag ccccccaggg atggatattc
    15181 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    15241 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    15301 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
    15361 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    15421 tctacctgcg taacatccag gacactaacg aaaccctctt ctaccgtctg gtaaacaatc
    15481 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    15541 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cggcacaata
    15601 tggacgatat tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg
    15661 gtgaacgcat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    15721 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    15781 tggatgtcgg aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta
    15841 atccgcgtat cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga
    15901 aacaacgctg gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
    15961 cgttacttaa ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg
    16021 cggcggtaac agtcggcaca ctgatcgcag caagccgcgc ggcaggtggt cagttaagcg
    16081 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    16141 tctcccagac ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg
    16201 tcgatcgctt tggcttgctg actgacaaga tgccgaacct gctgcctttc cagaccaaac
    16261 tggtgcagaa gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ctgtcactgc
    16321 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
    16381 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    16441 cgctgtctaa cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggaccg
    16501 aaggtaacgc gctggtcgcc acgggcagcc cgtttaatcc agtggtatgg aaagataaaa
    16561 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta
    16621 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgctgg
    16681 cgcagtattc accattggtg ctgaacggcg aaggtatggt actgccggaa ctgaaagata
    16741 ttcagaaagt ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    16801 tggcggtgaa aacctctgcc gaagccctgc aacaggccat tgacgataat ttctggcaag
    16861 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    16921 cgggctattt ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    16981 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    17041 gttgttacga aggatcatca tgttgacgtt acgtataaaa cactgcgctc actgaaacat
    17101 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    17161 aatggcgatt ttggtgacaa aatcggcgta attctgggcc atgaaggcat cggtgtggtg
    17221 gcagaagtgg gtccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    17281 ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
    17341 tcagttaaaa atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc
    17401 gccgattacg cggtaaaagt gccagatggt ctggactcgg cggcggccag cagcattacc
    17461 tgtgcgggag tcaccaccta caaagccgtt aagctgtcaa aaattcgtcc agggcagtgg
    17521 attgctatct acggtcttgg cggtctgggt aacctcgccc tgcaatacgc gaagaatgtc
    17581 tttaacgcca aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa
    17641 atgggcgcag atttagcgat taactcacac accgaagacg ccgccaaaat tgtgcaggag
    17701 aaaactggtg gcgctcacgc tgcggtggta acagcggtag ctaaagctgc gtttaactcg
    17761 gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg tcggtctacc gccggagtct
    17821 atgagcctgg atatcccacg tcttgtgctg gatggtattg aagtggtcgg ttcgctggtc
    17881 ggcacgcgcc aggatttaac tgaagccttc cagtttgccg ccgaaggtaa agtggtgccg
    17941 aaagtcgccc tgcgtccgtt agcggacatc aacaccatct ttactgagat ggaagaaggc
    18001 aaaatccgtg gccgcatggt gattgatttc cgtcactaag aggcctttgc tgcgactgcc
    18061 atgttcgggt cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg
    18121 cgtaggaagg aacaataatg atagtgttcc cagtgaagga acgctactga gagtaaagac
    18181 actaatgaaa atggccaatc atccccgccc gggggacatt attcaggaat cactggacga
    18241 acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgctccct caacggcaag
    18301 ccgattgctg acaggaaaag cagctttgac gccagaaatg gcgattaaac tttccgtggt
    18361 gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc
    18421 agaaaaaaca gtggatgtgt cgcgattacg ccgtctggtt acgcaataaa aaaatggcac
    18481 tttataaagc gccatttaga actgatgaca aacgcaacat tgcctgatgc gctacgctta
    18541 tcaggcctac acgttcactg caatctattg gatttgaagg gttttcatgc cggataaggc
    18601 gttcacgccg catccggcat gaacaaagcc tacgttgtct acaatatgaa aatggcgcct
    18661 tttacagcgc cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt
    18721 ttttcgcttc tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc
    18781 gacgacttac cttcccttcg atcatccctt taatcgatcc tttcacccaa aatgccatat
    18841 acatatggat caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc
    18901 tgtagcgaac aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc
    18961 cggtcaccag cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg
    19021 cgttgtactt accgacatcc gccactttat gctcattgcc tttcaatact tcgacaatgt
    19081 tcaacagcca cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca
    19141 taaacatcag tgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg cgtcccatct
    19201 gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg
    19261 acagcgccac caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt
    19321 tggtgcgcac aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc
    19381 ttattcgggc caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca
    19441 gccgctgcca gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga
    19501 tctttcggca gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg
    19561 tgcgtaccac cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc
    19621 agtttcgcca cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata
    19681 gccccggtcg gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg
    19741 cagagcgtgc atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga
    19801 cacccggcaa tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg
    19861 ttagcgtact gaatgattgc accagcagac gggcacgcct tcaggcagcc gggatcttca
    19921 cagtgcatac agccgtcttt acggatcagc cactccagct tgccgttctg ttcggtttcg
    19981 ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg
    20041 acgcagtgcc ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct
    20101 ttacagccga tacaggtgga aacgtcgata agttttgcga cttctgcttt gtaatcacgc
    20161 acctgagaag gcggcgtgat ggagttagtt gcggaccttt tgataatgtc ctgcgtttcc
    20221 atagccatct gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc
    20281 ggcgtttgcg agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta
    20341 cgcgcgacac cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgacca
    20401 tttacattca gcggtttcag acgacgcgtt accacagcca ccgcgcggat aaagccacgc
    20461 ttgctggaga cagtgacacg atcgccatta ttaatgcctt tcgccgccgc cagcgtttcg
    20521 ctgatttcca caaactgttc cggctgagca attgcgttga gcaatgcgtg cttggtccag
    20581 gtgtggaagt gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta
    20641 cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga
    20701 tgcagcgggt tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct
    20761 tccgccattt tgttgatggc aaacaggcgt cccatccctt ccggctgcat gataaacggc
    20821 ccggttggcg tacccggtgc ggcattgccg aagtcaggaa tatcgttacc cgtccacttg
    20881 ctgccgttcc actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc
    20941 gaagcacggt tgtacagcac gcgacggttg agcggccacg cccaggccca tcccagcgta
    21001 ttccccagac cggacgggtc ggagttatcg cggttagcca tctggttgcc ctgctctgtc
    21061 cagctaccgg tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca
    21121 aagctactca gcaactgacc tttcttcgca ataagcacgc cattagcgtc atagagatct
    21181 tccagcgcgt agccgttgtt ctctttagcc acttcgtcag attgcggttc gtgcggctgc
    21241 ttgtagttcc agctcatctt catcagcggt tctacgcctt taccaccttc ggactggtac
    21301 agctcgcgca gatgatggta gatacccgcc agaatttcac cgtcgttacg cgcttcgccc
    21361 ggcgcgtcct gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca
    21421 tcttcttcag caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga
    21481 tcgacatcgt tcgactcacc gtggttctgc cagaaggtag aggtttcagt caccagcgga
    21541 tcgataacca ccatgtactt cagcttgctc aggcagctca ccactttgtt tttgtccggg
    21601 aaggacgcaa ccgggttaaa gccctggcag aaataaccgg tgactttgcc ttcatccatc
    21661 atgttgaaat acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc
    21721 cagttgttct ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac
    21781 ttcggatagt tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct
    21841 tccagatacg actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg
    21901 ctggtagaga gcaggcctaa gtcagtcaag ccctgaatgt tggagtgacc acgcaatgcg
    21961 ttcacgccgc caccggccat acccatgtta ccgagcagca actggatcat cgccatagta
    22021 cggatgttct gcgcacccac agtgtgctgc gtccagccca gcgcgtacag gaaggtggtt
    22081 gtgcgatccg gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt
    22141 ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc gggaaacgtg ctctttcagc
    22201 aggttccaca cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca
    22261 tcgaactgat agttccagga cgatttatcg tattgacgtt tttcagcgtc gtagccgctg
    22321 aacagaccgt cttcgaaagc aaaatcatca cgcaccagca ggctggcgtt ggtgtaatgc
    22381 ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac gccagacagg
    22441 aacgtaatgt ccgtaccgga acgaataggc gcgtaaatat ccgccacaga agcggtacgc
    22501 gtaaaacggg gatcgacaac gatcaaggtt gcgtcgttgt tgtttttcgc ttccatcgcc
    22561 cagcggaaac cgacgggatg cgcttcagca gcgttaccgc ccatcaccat cacgacgtta
    22621 gcgtttttga tatccaccca gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga
    22681 cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg
    22741 gagcgggcaa atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc
    22801 ataccggtag aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta
    22861 gcgtcacggt cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg
    22921 cgctgccatt tgtcagaacc tggcgcacga tattccgggt agcgcagacg gttttcactg
    22981 ttgacgtaat ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga
    23041 tccgggtcac cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg
    23101 ctatacatca atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta
    23161 gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat
    23221 gccgctactg ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg
    23281 acgtccattg ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa
    23341 ttctgaatct ttttgcgcga aatcaaaaag aacatctgtt tgccacaaaa taatccgcct
    23401 tcgcaaattg acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt
    23461 aaaaaaatgc tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca
    23521 tgacacgaca aaaagcaacg ctcatagggc tgatagcgat cgtcctgtgg agcacgatgg
    23581 taggattgat tcgcggtgtc agtgaggggc tcggcccggt cggcggcgca gctgctatct
    23641 attcattaag cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcaaatcc
    23701 cgaaaggcta tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc
    23761 tttccttagg gtatgcggcg acccatcatc aggcgattga agtgggtatg gtgaactatc
    23821 tgtggcccag cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggt
    23881 tgattgtacc tggattatta ttagccctcg tcggcgtctg ttgggtgtta ggcggtgaca
    23941 atgggttaca ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc
    24001 tggcgttcat tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac
    24061 gcggatttaa tggaattacc gtttttgtcc tgctaacggg agcaagtctg tgggtttact
    24121 attttcttac gccacaacca gaaatgatat ttagcacgcc cgtcatgatt aaactcatct
    24181 ctgcggcatt taccttagga tttgcttatg ctgcatggaa tgtcggtata ttgcatggca
    24241 atgtcaccat tatggcggta ggttcgtatt ttacgcctgt actttcctca gcgcttgcag
    24301 ccgtgctgct cagcgccccg ctgtcgttct cgttctggca aggcgcgctg atggtctgcg
    24361 gcggttccct gctctgctgg ctggcgacac gtcgtggtta aaataactgc cgggtctacg
    24421 gcccggtagt taatcagata aattccgaca atattaaaaa aataaatttc aaataccagt
    24481 aattcacctt atatctcatt tcgacttcga tcacatatcg ataaataata ttatttcatc
    24541 tatttttctc a
//
LOCUS       DBBGLQ010000062        24136 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.062, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000062 DBBGLQ010000000
VERSION     DBBGLQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.062"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(132..779)
                     /gene="gloC"
                     /locus_tag="ACIYSB_003422"
     CDS             complement(132..779)
                     /gene="gloC"
                     /locus_tag="ACIYSB_003422"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGC5430401.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVIFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(806..1354)
                     /locus_tag="ACIYSB_003423"
     CDS             complement(806..1354)
                     /locus_tag="ACIYSB_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGC5430402.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1535..3382)
                     /gene="ldtD"
                     /locus_tag="ACIYSB_003424"
     CDS             complement(1535..3382)
                     /gene="ldtD"
                     /locus_tag="ACIYSB_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGC5430403.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAILAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQMTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3643..8103)
                     /gene="mukB"
                     /locus_tag="ACIYSB_003425"
     CDS             complement(3643..8103)
                     /gene="mukB"
                     /locus_tag="ACIYSB_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGC5430404.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8103..8807)
                     /gene="mukE"
                     /locus_tag="ACIYSB_003426"
     CDS             complement(8103..8807)
                     /gene="mukE"
                     /locus_tag="ACIYSB_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGC5430405.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8788..10110)
                     /gene="mukF"
                     /locus_tag="ACIYSB_003427"
     CDS             complement(8788..10110)
                     /gene="mukF"
                     /locus_tag="ACIYSB_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGC5430406.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10107..10892)
                     /gene="cmoM"
                     /locus_tag="ACIYSB_003428"
     CDS             complement(10107..10892)
                     /gene="cmoM"
                     /locus_tag="ACIYSB_003428"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGC5430407.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAECGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11028..11807
                     /gene="elyC"
                     /locus_tag="ACIYSB_003429"
     CDS             11028..11807
                     /gene="elyC"
                     /locus_tag="ACIYSB_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGC5430408.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            complement(11784..12677)
                     /locus_tag="ACIYSB_003430"
     CDS             complement(11784..12677)
                     /locus_tag="ACIYSB_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGC5430409.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12831..13577)
                     /gene="kdsB"
                     /locus_tag="ACIYSB_003431"
     CDS             complement(12831..13577)
                     /gene="kdsB"
                     /locus_tag="ACIYSB_003431"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGC5430410.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGIGVDTPEDLERVRAEMR"
     gene            complement(13574..13756)
                     /gene="ycaR"
                     /locus_tag="ACIYSB_003432"
     CDS             complement(13574..13756)
                     /gene="ycaR"
                     /locus_tag="ACIYSB_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGC5430411.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13808..15040)
                     /gene="ycaQ"
                     /locus_tag="ACIYSB_003433"
     CDS             complement(13808..15040)
                     /gene="ycaQ"
                     /locus_tag="ACIYSB_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGC5430412.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGCC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15077..16063)
                     /gene="lpxK"
                     /locus_tag="ACIYSB_003434"
     CDS             complement(15077..16063)
                     /gene="lpxK"
                     /locus_tag="ACIYSB_003434"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGC5430413.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(16060..17808)
                     /gene="msbA"
                     /locus_tag="ACIYSB_003435"
     CDS             complement(16060..17808)
                     /gene="msbA"
                     /locus_tag="ACIYSB_003435"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGC5430414.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17845..20109)
                     /locus_tag="ACIYSB_003436"
     CDS             complement(17845..20109)
                     /locus_tag="ACIYSB_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGC5430415.1"
                     /translation="MKITTVGVCIISGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPASGLVMYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSLPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTMVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHIWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQLQGYQWIDTPHQGQTTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20317..20601)
                     /gene="ihfB"
                     /locus_tag="ACIYSB_003437"
     CDS             complement(20317..20601)
                     /gene="ihfB"
                     /locus_tag="ACIYSB_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGC5430416.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20761..22434)
                     /gene="rpsA"
                     /locus_tag="ACIYSB_003438"
     CDS             complement(20761..22434)
                     /gene="rpsA"
                     /locus_tag="ACIYSB_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGC5430417.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22545..23228)
                     /gene="cmk"
                     /locus_tag="ACIYSB_003439"
     CDS             complement(22545..23228)
                     /gene="cmk"
                     /locus_tag="ACIYSB_003439"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGC5430418.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23401..>24136)
                     /gene="ycaL"
                     /locus_tag="ACIYSB_003440"
     CDS             complement(23401..>24136)
                     /gene="ycaL"
                     /locus_tag="ACIYSB_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGC5430419.1"
                     /translation="ATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIAN
                     QGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAMANGC
                     VRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVASQ
                     LSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGR
                     TQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         5750 a         6640 c         5926 g         5820 t
ORIGIN      
        1 cgtcccctgc aatattttga atttgcacga ttttgtaggc cggataaggc gctcgtgccg
       61 catccggcat aaacaaagcg cactttgcca gaaatccagg cccgcattaa aagcgggctt
      121 ttttatgtgc cttaccagac gggcatttcg tcttgcagga agggattatg caggcgttca
      181 taaccaagtg tggataatgg tccgtgaccc ggaataaata tcacgtcatc ccccagtggc
      241 agcaatttat ctttgattga agaaatcagt tgattatgat cgccacgcgg gaagtcactg
      301 cgccctactc cgcctttgaa aataacatcg ccagaaatca gcagctttgc ccgatcatca
      361 aaaaacacga catgacccgg cgtatgccca gggcaatgta acacctgtaa agtcacattc
      421 cctatgctga tggtatcgcc ttcgttcagc caacgatctg gcgtcagcgg ctggcactct
      481 tccagaccaa acatacgact ttgcgcaggc aagccttgca gccagaactc atcttctttt
      541 tccgggccga aaaccggcac gccgtaatgt tgcgccagtt ccgccgctgc gccaacgtgg
      601 tccagatgac catgcgtcag caggatctgc atcagtgtca ggccgctgtc atcaacttcc
      661 tgtttgattt tttccgcatc gccgccagga tcgaccagtg cggccagacg ggtttgttca
      721 caccagatta atgaacagtt ctgggagaat gcggtgaccg gaataatacg atagttcata
      781 ctgcccctgt ttcgttaagc aattactacc agtgccgtgc tggcccggta tcaatatgca
      841 caaagttact acgtgggtaa tatcctacac cacctgcgcg catagataac gcggctttgc
      901 gaatattgct taacgcgata ccttcaatat ggaaatccat cgcctggcct ttagtgtgat
      961 agcttttctt cgctactcca cggctgcggg cgcgtagttc attgttggta tcaatagaac
     1021 gataaccgga aatgagttgc accggtttgc gcgtgcctaa caacccttgc aggcgataca
     1081 actggtcgaa taatcctggg tcgatggact ttattttgtt cgcgcggtaa tcgcggaaaa
     1141 aatggttaag ttttgccaat tcttcctgaa tatagcctct gccatcgaaa aactccgctt
     1201 tgattgactc tccggtatga agattattga gtgtcaaaat gcgcgggcgt ggggtagaga
     1261 gtgttgcaaa cgcaggggtc ggcaggatgg cggcaccgag tgcaacgcca ccaagcgcca
     1321 gcaatttgcg gcgattagcg tcgaatttgt ccatgataat caagtctaca ggtcaatgtt
     1381 atcgtttata tgcacttctg gcgcacaaga ggcaccttaa ccgcccgtaa agcagacgtc
     1441 aagatgactc aaccccagca gtgacggggg ctgcagagaa tcgcccccaa tcatgctaat
     1501 tattacgaca actgatttcc ccgaactact tcatttacct gattaattgt tccgctttcg
     1561 atacgatttg cgagctggat cgcgcaggca gatcataatt gtaaatatct gtacgatact
     1621 gggtacgacc atctgcacca acaaaggccg tcaggtagta gagattcacc ggaatcgact
     1681 gccgaatatt gacgtaacgt gtatcaccct gcttcagcgc atcagaaata cgtttgtcat
     1741 tccagcctgc atcctgcaac agcatattcg ccagatcgga agctttattc actcgtacac
     1801 agcctgagct caatgcgcgt gtatcacgct tgaacagatt gtggttcggc gtgtcatgca
     1861 aataaatggc ctctgaactc ggcatattga atttatagcg ccccagcgag ttccgtgggc
     1921 ctggagcctg ctggaagcgg aacggtaaat tcgaggccgt gattgtagac cagtcaacct
     1981 gccatgggtc aatcgcttct ctgctgttcc agccgcgcat caccgtatag ccatggcttt
     2041 cgagatatcc cggatcgttg cgcacttttg gcagaatatc tttgcgtgcc agagttggag
     2101 gtacgttcca cggcgggttt accactacgt tgttaagggc actgctcatc atcggcgttt
     2161 tgcgatcggg gcgaccgaca atgactcgcg aatccagcac ctgattgccg ttctgatagt
     2221 agaccagcga ataggccgga atgttaacca tgatcccggt agaaagctct gttggcagca
     2281 agcgcaatcg ctggatgttg agagccaaca cgccagcacg ctgggcgggc gttacgttta
     2341 accagtcacg tgttgccggg ccaatagcac catctgcccc caatccttgc catgcctgaa
     2401 aacgtttaac ggcttccacc agttcattat cgtaggcggc acgaacggca ggcgcaggtt
     2461 tactacgaga cgtcatttgc ttgtccatcg gcttagtttc tgctgtttca acagtaacag
     2521 cggatgggct gactaccgcg tcagttggcg tgtcatcgcc aggtagagta attttcggcc
     2581 ccccgtccaa catgcctgtg cgttgcaata tttcgcgcaa cgccggtacg tcgttactcc
     2641 actgccctgg gcgcaacgtt gctttgccgg tcagttgggg ccacggtttg gtgtcactga
     2701 gtaaggccag taacgattca tgcatcgccg catattgcgg atgctgcggt gccagtcctg
     2761 caacaaacgt aggcaattga cctttatcca gcgccagctg ccattggtta atcaccgaga
     2821 gcggcggcgt tgcaagtgca taaggtttac tgctatatag ccagcgagtg cctttgaccg
     2881 gaatatttgc aatgaaatgg agatagccca tcatcgcatc agagagcact acgtcgcgtg
     2941 ccatcccgtt aacaccagga tcggtcagta actctaccca tttattaaac tgcggctgga
     3001 aaccggcaat cgccacctct gccagctgtt gctggaaggc tttaacagca tcacggtttt
     3061 cccacatggg ttgcatatcg cgtgcggcat acaacagttg aagctggtta agataaaccg
     3121 gcttgtaacc tgcgggtaat tgagattcga tttgcgtgcg ggctttttct gccgcacctt
     3181 caggcaatgg ctggatcccc gccaatattg ccgtggcttg cgcctgcggc agtgcctcgc
     3241 cctgttcact gacagccacc gggctgtcgc cagggattac ttcaggctca tcggcctgcg
     3301 cattgaacag tggagcgaat gttacggcca ggcacaaact gattgccgac agccgacgac
     3361 cacacatcat attaagcaac atcccttgcc ccctgttttt atcatactct gaatagccag
     3421 gctgttattt tacgacttgc ctgtacaacg ccaatactca cgaaagtatg acgagcaagc
     3481 ctgttactca tcaggcttat cttcagtata taaacgccga tacgttttta cataacctaa
     3541 tgtaaagaag tttaaagaaa agcgcgccgt aatgtgccac ttttttagtg cagaaacgga
     3601 gttttcggaa aaagaaaagg cggcattgct gccgccttaa ttttaactcg cctgagaagg
     3661 cgcttcgtca cttcctggaa gcgtttcagg gagttgcggc gcaaatcctc gcaggccgac
     3721 gacgtgaacg tgttcggtat tctggaagac tttacgcacc agtttatagg tggtgccttt
     3781 ctccgggctg atattttccg gcgctgcgat gatgagctgc atttgcaaac gttcacacaa
     3841 ttcaaacagc gtggcgatag aacgagcatc cagtcgcgct gcttcatcga ggaacagcag
     3901 gcggcaagga gagatatctt taccgcgcag gcggcgagat tcatcttccc agctttgtac
     3961 caccatcacc agaatcgaca taccggtacc aatcgcctca ccggtcgaca atgcaccaga
     4021 ctctgcgcgc aaccagccat cggaaccacg attaacctca acttccattt ccagatagtt
     4081 gcggtaatcc agcagttctt cgccaatggt ctgcggcgtg cgctgcccca tatcaatctg
     4141 cggattaaga cgctgataca atttcgccag cgcttccgag aaggtcagac ggttgctgtt
     4201 aaacagatcc tgatgctgct cgtgctgttc agagagcaca tccagcagca tggcgtgcgt
     4261 ttcacgcacg ttcacgttca gacgtacgct gttcacctga ccaaacgata cgttctgcaa
     4321 cccctggttg agcatacgga tacggttctg ctcgcgctga atggttttgc gaatgatgtt
     4381 cgccacgctg cgggaactga tcgccagttt ctgttcgcgg gaggttaact cctcggtcag
     4441 tcggctaagt tcgatttcca tctgctcgat ggcttccacc ggatcatcgg tacgaataat
     4501 atcctgacga atacgttcac gcagatgctg ataaaccgcc acgaagaact gaattttacg
     4561 ttccggacgt tttggatctt ccgacatgcg cagcacgtcg cgcagatgtt cgttatccgc
     4621 caccgccaga cgcagcgcac ccaatgcctt atccgacata gagcgcaaat catcagcgga
     4681 gagataagcc agctcgcgac ggtgtaagcg ccgttcaacg ccgttatctt tcaccatgcg
     4741 catcactgca caccagcccg ctttggcggt cactacctgc tcgcgcatct caaagtaatc
     4801 ccgctccagc ttgcgcagtt tgcgggtcag attgtccatc tccgcttcgc agaaagtcag
     4861 tgctttttcc agctggttgc ggcgggaccg gttattactc agttgcgcat gcagctcgtc
     4921 acggcgaatt cgcgcccgct cttctgcccc gctatcggcg cgcacgccga tatcctgcaa
     4981 ttcacgttgc agatcgttga gtagctcttt tttggtgtcg taagaacttt tcagcgaagc
     5041 cagcacctgg ttgtactgac tcaactgcgc tgcgtgacca cgcaacgctt cacgagcgcg
     5101 agtacgctcc gcttccgcct gttccagacg ttcacgcagc ttttcgttga gatcgctatt
     5161 accgctcagc atttctgccg agtcagaata gctaaagtgc gcacgacgct gcaccacttc
     5221 cgtcagggca aacgcctgct gacgggcatc gcgctgcatc tgctgagagt acgcgtaatc
     5281 ttcttttagc tgttcgaact gttccgggtc gctctgcaat accgaaacga tcggttccag
     5341 tttcgccagt tgattgccaa actgctgaac aaaacgcgcg gcttcctggg cttcatccag
     5401 acgttcgcgg atttcatcga cgcgatccgc caggctgtca tcagccaaca ggttgagacg
     5461 cggcagaatg cggttcagcg ccgtaacgcc ttctttcgcc tgctcaaact gaatacgctg
     5521 ctgctggtta tcattttcat gattactcaa cgcccgctcc agttcgacgc gacggctgtt
     5581 cagttgacgg atttctgctt ccgggtcaga ctcaaacgca accgccagat gactgccgat
     5641 aaagcggctg aacgcctgat gcagacgctg agttttctgt acatcaaagg agagcgtggc
     5701 gaagcgttcg gaaagcactt cacgctcggc atggaggctt tcaatacggc tttcacgcgc
     5761 agcacgacca aacagcggca cttccgggaa acgtgaataa cgccactgac gatcggcgat
     5821 tttcaccact accgcttttt ccagctcatc aacgctgaac acgctgtcat cgaatgactg
     5881 cggatctcct tcgatcagat agagatcttc cgggcaatcg gtcaagcctt ccaggtgttc
     5941 agttacctgt gacagatctg gcaccacgat ggcgtggcgt gacgggccat acagcgctga
     6001 gaagtacggc gcatcttcca ggctaacgtc gtcataaatt tctgacagca gcacaccacc
     6061 aaaacgctcc gccagcgcgt tcagacgctg atcttcagag ccgccaggct ggcttaaacg
     6121 ttcgatctct tcatcgacgg cgtttttgcg cgcgcccact tcatcgcgtt caacaatcgc
     6181 ctctcgctca cgctccagca actgttgcag atattcggtg acgtcctggc tggaggtaaa
     6241 ctcttcgccg cactgttcgc tcaactggtt gagactgttt tgcgctgcca gccaaaccgg
     6301 cgcacgctgc atcaaactct gaatgcgaga ctgcagctgt tcctgttcct ggcgcagtgc
     6361 catgcgctct tcacgggcgt tagacacgct atcggaaaga gaggcaatgc gcgcttccag
     6421 ctcctgatgc agagcttcca gttcatcaat atcaaaattc ttgccctgac gtttgcagaa
     6481 gtctgccagc aggcgttcgg cttcttgctg ctcgcgcaga cgctgttcca gttcacttaa
     6541 gcgcatccgc aacggttgaa cctgctctgc caggtgacgc tgatcgaccc cttcgcgcaa
     6601 tagttcgcga gcgacatccc acgcctcgtt acgcgccagc gggccgttga ttgccaccac
     6661 cagctgataa gcctgctcaa actggctgtg cgcggtttgc gccatgctca ttttctgctc
     6721 aagagagagc attttttcag tcgcttccag ctctttcgcc tggaaggttt ccagccattc
     6781 ggcggcgctg tcggcggtta agtccggcag atggcacagt tctttggcgc gattaagcgc
     6841 agcaatcgcc tggttatact ggatcgcgcg cgtttgctgg acgtccagcg cctgctggta
     6901 gtcagcaagc tggcttttca gctcgtccac ttccagttcg gcagcttccg cgcgagcctc
     6961 gttctcttcc tggcgttcga tggcttctgc caccacttcg ttttgctctt ccagacggat
     7021 ctgcagctca tcgagatccg cttcgtagcg ttcgatcttc tcctgctgac gcagtgcggt
     7081 ttgcaccagg ttcaggtgat cgctggccgc ctgataatcc gcttccagat cgccttcggc
     7141 accgttgtgc tctgccagct cacgcgccat atcgacgtgt ttgtactgct cagccgccag
     7201 ttgctgacgc gaagtatgta actcgcgacg aaactccagg gctttgtcga gatggacacg
     7261 gcgctcgttg gcgtgacgca tgtagtccgc cgccacgtag ttggtagctt cgctgatcag
     7321 atgcttaaac aggtcacggt ccgactgggt gacacgaatc gcttccagcg tcatgcggtt
     7381 ttcacgcagc gccgcttcca tgtcctggaa cgctttacgc acgccgctgt tttccggcaa
     7441 caggtagtcg cgcagagaac gggtaatggc actggagatc ccgccataca gcgaagcttc
     7501 gatcagacga tagaatttgc tacggtcaga tgcggagcgc agacgacgcg cgatgatgcc
     7561 cagatcgaac atcagcgagt ggtaatcggt aatggagttg aactgtttaa actgcacgcc
     7621 ttccatcgcc tcgagcttgt ctttcagctc gttaagcggc agcacgcgcg cctggcgttc
     7681 gttcagggtt tcggtcacca gctgtgtcgg ctgcaccgac atcggcagtc cctgaatggc
     7741 aaatggtttg atatccactt tacgatcgcg tccggcaacc tgttgcagac gcacaccgac
     7801 caccacgcgc tggtggcgcg agttaatggt gtcgagcatc gaataacaga cacccgcttt
     7861 cagcttaccg tgcagacctt tatcgcgcga accgctggtg gccccggctt ccgtagtgtt
     7921 acggaaatgc agcagggtca ggtcggggat cagcgccgta acgaacgccg ccatggtggt
     7981 ggatttaccc gccccgttac cgccagaaag cgtcgtgacc agctcgtcaa ggtcaaaagt
     8041 tcgggcaaaa aagccgttcc agttaatcag cgtcagtgag cgaaatttac cgcgttcaat
     8101 cattattctt cctctccgct atctggctga ttctcttcgg tttcatcgtt gagttgcaga
     8161 tgattttcaa tcggcattgc ttcgccatca cgaatcaggc gacgctgtgc ttcacgggga
     8221 tcgtcgccag cacgcacatc ggctccgaag cggaacaccg attcggtaat gcgaaacttg
     8281 ctgctgtcgt ggcccataaa ccacaccatg cctaaacgac gcagacggtt gagcgaagaa
     8341 cgtactttct cctgcaactt ctgacggtca acgtctgaac cggttgaacg gttgttcacc
     8401 agtttcagca gttttgcttc atcggccagg gtgagcagtt cgtcgtacag ttcctgctgg
     8461 gtgaaaatcc cctcattcgc cagccgttcc gggctgagat agagataaca gaggattttc
     8521 ccgaccatca tatccagttc cgacaagacg gaacgcggga taagagtggt ggaacgtggg
     8581 cgtaaataga agaacccttc cggtgcgcga ataagctcaa cgttataacg cgcgtaaaac
     8641 tcttccagat attcctgaaa atccatcagg aatgcatgat tatccagttc gtcgaggcca
     8701 atatggcggc ctgaacgtaa ggcgctgtcc agcgccggaa ataacggatt cgccagcgcc
     8761 tgcgccagct taaccggcat cacttgttca atatttgtcg atgacatgcg cctgtacctt
     8821 ggctccgtaa tcattaatcg gctgccattt cgctggcagt ccggtgaaat ctgcttgcgc
     8881 tacgccaaga cgtaccgcct gatcaataac aatacgcgca acgtcaaagt gacgtgcacg
     8941 cggatactgt gacagatatt cgcgtaccac cagaccaaga tccagcggca cttgtctggt
     9001 tttgtacacg gcaagttgtt cttcgatgat cgccgccagc tgttcgcgga tctcgttaaa
     9061 ctcttcgtat tccagatcct caggaagttc cccagtcact tcttcatcgc gcagtgccat
     9121 ctcttcgtca cgcatatcca gcagacgatc ggcattggca taagttagcg cccacggctc
     9181 atcaaaatag gtttgtaccg actgacgtaa ccgctgagca aagacgcggt ttttatccat
     9241 atcgatcgcg gtacgaataa atttgtgtac gtggcggtcg tagccaatcc acaagtcgat
     9301 ggattgctgg ccccaactga taatacgatc gagtttgctc tgcagatcga acaccagacg
     9361 atcgacgaaa tgcagatcgt catgggtcat cgtcgcatcc tgaatgcgca acagattagc
     9421 ctgcaatttg tcgcctgccg cttccagcgt atcctgcaat tcacgcagcg ttccggaagt
     9481 ttccgaaagc aacaattcac agctggaaat cgccgcccgc cagtctttgt tcagcaactg
     9541 ggcgatatcg tccttcacct gctgctgctg ttcgtccatc agacgttgcg tcaggtcgat
     9601 actgtcgaaa atttctgcta ccgaatattt cagtggcgca tagacattac ggtgccagtg
     9661 aaattcatca ccgccctctt cggcggcatc tgctgcgcgt ttgagctcac ccgccacaat
     9721 cgacaactgc atagaaagac gcagcgtaga aaactcgcgc tgacggatgt agtagtcagt
     9781 aatgccgatg ccgagcggcg tcagacggta aattgcgttc ccttccgcct gctcgctggt
     9841 aaagcggttc agcagacgtt gacgcaccat gtcgttgatc gcgttattgg cgcgcacgcc
     9901 gatggtttcg ctggtttgct caaacgcatc actcacatgg cggaatgcat ccaccagctc
     9961 gccttcactc atctcaccat ccagacgctc gccgttcagc gtggcaaccg ccagcagaaa
    10021 agagagtcgg tctaccggca gcgagatgga gaagtcattt tttctggccc aggcaaccag
    10081 ttcggggact gtctgggaaa attcactcat actttatcct tgctctgcgg tttgcgcgcg
    10141 gtgacatgaa tataacgccc cagggtaata tacggttcct gacggcaata acgcgtttct
    10201 aattcaagta atgcttcata gcagtcgcgc tgctggtgtt tctcgcgcag ataatcatga
    10261 aacacgcgaa cgcctgtctt acccataatt tgccaaccag cttcttccag ccacagataa
    10321 acctgcgccg ggtcgcgtgg ataatctggc gaaagcgtcc gttttttctt tttcggcatt
    10381 cccgcctgca cgtaatcaaa attcccggcg accatgttat gcatcaacaa accatgcgca
    10441 ttgtagaaca ttaacgacaa cacgccgcct ggacgcaaca ctgaccacag ggtctgcaat
    10501 acactgcggg gatcggccac ccactcgagc accgcatgga acaatatcag atcaacgggc
    10561 gtttccaaat gcgaagcaac atcctgagcg gcgcaatgta taaattgcat gttgtcgctc
    10621 acaccttttg cttctgccgc ctgttttgcg cggtcgatca tctgcgcaga aagatcgcat
    10681 aaaatgacct gatgcccaca ctcggccatt ttgattgcgg tctgcccttc tccaccgcca
    10741 gcatccagca cacgcagttt ttgcgggccc atttccgcca gcacgcgatc gagatcctgc
    10801 cacagaatag cctgtcgaag ctgccctttg gtggtgccgt aaatgttacg ggaaaacttt
    10861 tccgcaatat catcaaaatt gcgatcctgc attgctttct ccaccggttg gctaagacgt
    10921 tttctgataa agacgctatt ttgtcacagc cacggcgaga atgaacccgt ctggtgtaat
    10981 atcgctggcg atcccttgct atatggttaa aaaaggaacc agaaaggatg ctttttacac
    11041 tgaaaaaagt gattggcaat atgttgctgc cacttccgct aatgttgttg attattggcg
    11101 ctggcctggc gctgctgtgg tttagccgtt ttcaaaaaac cgggaagatt tttattagca
    11161 ttgggtggct ggcgctcttg ttattgagtt tgcaaccggt agccgaccgc ctgctgcgtc
    11221 ccatcgaaag cacctatccg acatggaata attcgcaaaa agtggactat atcgtggtgc
    11281 tcggaggtgg ttatacctgg aacccgcagt gggcaccgag ctctaattta atcaataaca
    11341 gtctgccacg cctgaatgaa ggtattcgct tatggcgcga aaatcctgga tcaaaactga
    11401 tcttcacggg aggcgtagca aaaaccaata cggtgagtac agcggaagta ggtgccagag
    11461 ttgcgcaatc gctgggcgtg ccgcgcgagc aaattatcac cctggatttg ccaaaagata
    11521 ccgaagaaga agctgcggca gtgaaacagg cgattggtga cgcccctttc ctgctggtga
    11581 cctccgcatc acatctgccg cgggcgatga tcttttttca gcaggaaggg ttaaatccgc
    11641 taccagcccc ggcgaatcag ttggccatcg actcgccgct taacccgtgg gaacgggcaa
    11701 tcccttctcc ggtctggttg atgcatagcg atcgtgttgg ctatgaaacg cttggccgta
    11761 tctggcagtg gctaaaaggt tcgtcaggcg agccacggca agagtgattt tgatgccaga
    11821 tcgaagttgc gccgactaaa tcgtccggta ttaaccagtt gcgcaacttc atcccataac
    11881 acatacaacc aacgccgcca gatgaacgac tccgccactg gcgcgcgttg caggtaactc
    11941 cagagcaaat cttccgccag agaattatcc attagtcgga acagttcgta ttctcgcggt
    12001 gcccaaagca ttagtcccgg cccgaccatc gccagtaact gatcgctgcg cgaatctttc
    12061 aacatgctgc gtaaacagaa gttaccgtga atcagcacac aattgtcgtt aaagccttca
    12121 aataatgccg ggagacattc gcgagtgcgg aacaggatcc gcttatcctg catcgttaga
    12181 ccggtgttat tgaactgatt gagcgtggtc catagcactt caacatgttg ccggtaccat
    12241 gagggccaga aattttcctg agtattgtcg accgcgccga cgcaaccgcg actgtcctga
    12301 cggtgccagg ccagtaaggc ttcaacgatt tggtctttga gctgttccca gcgttctggt
    12361 gttcgggctg gtgcttccac cgaaacacca cgcatccgct ccagtagcag gacatcgggg
    12421 ccggggtgtt cttcatgcgt catcacacca taaatagtcg gcatacggac agtcccactt
    12481 cttgccagca tggtggtttt ccatgccagc tgtcgggctt ttccgggcgt actaaagctt
    12541 cttgccatta acggcattgg gtttccctgg ctgtcataca aagcccacaa cgccgtatcc
    12601 gctttttcat tgacgcactc aatacgactg agtttttcgc ccagtaaatg gcttaattcg
    12661 gcacgcagct gttccattca gattaccctc ataaaagcta ctgctttcat aatgagcgcc
    12721 aatataaaag atgtcacctg aatgagatca attgttgaaa agaaaaattg aggaaaatcg
    12781 taacggtacg acactcctcc caaaattgtc tggagtgtcg tgaagtgaaa ttagcgcatt
    12841 tcagcgcgaa cgcgctcgag atcttcaggg gtatccacac ctatgccagg aacttcatgc
    12901 gcgacagcaa catggatttt ttcgccgtac cacagaacac gaagctgctc taacatttcg
    12961 atgtgttcta acgggcttgg ctgccaggtg acgtaacgac ggataaagcc tgcacggtag
    13021 ccataaatac caagatgacg caggaagtta tcgccaacgg tttcaaggtc ttttgcaaaa
    13081 cggtcacgat cccacggaat ggtggcgcgt gagaagtaca gagcataccc ttcagcgtcg
    13141 agaacgactt tcaccgcatt cgggttaaac gcttcttccg cattgtggat tggcaccgcc
    13201 agagtcgcca tacccacctg acgctgagcg aggttatcag caacctgacg aatgatggtc
    13261 gcagggatca tcggttcatc accctgcaca ttaacgatca ccgtgtcgtc gctgaatgcg
    13321 catttttcga caacttccgc cagacgttct gttcctgact gatgatcggc gcgcgtcata
    13381 catacttcac cgccagcggc ttcaacggcg cgggcaacat cctcatgatc ggttgccacg
    13441 atgatgcgct cggcacctga ttcacgcgcg cgttcaagaa catgaacaat catgggtttg
    13501 ccgttaatat caaccaatgg tttaccgggc agacgcgtgg acgcgtagcg cgcgggaata
    13561 atgaccacaa aactcatgat ttactctcat cagcagtcag cacgcgggct tccgtttcca
    13621 gcaacactgg aatgccatca cgcagcggga aagcgaggtt gtccagtttg caaataagct
    13681 cttgtttttc ctggttatac caaagttttc cgttgcaaac cgggcaggca atgatttcaa
    13741 gcagacgatg atccatagtt cctccagatg gatcgggtta tgaatgcata aatcttatca
    13801 taatcattta cgcgacgggg tctatttccc agccggtgcg gcaatcagta aagaggcctt
    13861 gcgggcagca tcctaatgtc acccgcgttg cctgctgcca gttagcgaaa tcagtaatcg
    13921 cctgacgtag ccctttttgc agcgttgtcg ttggtttaat accttcctgt aaccacagag
    13981 agataacttc aaggatgcct gtctggcgat gcattttggc atccattcgc ccaactaatt
    14041 gcccacgatg taataatggc agaacaaaat agccatactg gcgtttcggc gctggggtat
    14101 agcactccag ccggtagcta aaatcaaaaa gctgctctgc gcgtttgcga tcccagacaa
    14161 caggatcaaa aggcgaaagt accgcgctgt gagttgcagt gagctttccg gccagcgcac
    14221 gctcgagtag cggcagcaaa tcagcatgca gccaaagatt gcccaatttt tcaacatgca
    14281 cagcaatgat ttgctgctgt tcagccctcg cttcgcgcca cgccgccagt gccggacgtt
    14341 tcagccgata gtaatctgcc agccactgtt cgcggaatat tcccaggcta cgcgcactgt
    14401 tatccagcat gatgatttct gcttctgttt gcgaaacgag atcgcgctca tcatcccagt
    14461 caggcatgac acggtgggtt aaatcataaa cgcgctggaa gttgcgccgt tcaatcacca
    14521 tcacctttcc ggcagtaaat aaaccttcca gatgccgttt atgcggcttc cattcccacc
    14581 agccgcttgc acctttacga ggatgctcaa aatcggctga acgtaccggc cccttatcat
    14641 gaatatgctg aattaactgt gcaatttccg cctcatgttc ctgcatccag gcgtctttgt
    14701 atttccagcc cattttttca ggtgccagca tgcggtggcg aataagacga aagtcgctac
    14761 gcggcataaa gcaggcttca tgcgcccagt attccattaa ttcgccacgc gccagagact
    14821 catccagcca ctgggcagga taatttccca gacgactgaa aagcaccaga tatggactac
    14881 gggcaacaat attgatggta tcgatttgca acaaggacat gcgggagatc gttgccggaa
    14941 tatcctccaa cgacgctcga cggcggggtt tgtttaacag gccttgcgcg gcaaggtgaa
    15001 gattacgcgc atcagcaagg gagaggtgcg gcagcgacat tcatgactcc atcaatcgaa
    15061 cgctgccgcg gcgtaactag ttgccagaag ccagcgaggt tagttgcgca agcagtttcg
    15121 ctggttcatc acctgaaagc tgtgcgtcaa cgggcaaata ccaccaattt tcttctgcaa
    15181 aggcccggca tttcaccgca tctttttcag tcattaccag cgtttgcccg gtgcttacca
    15241 acgcgctgac atccgcatgg ttcaaagact gatgatcggc cagcggtaca catttttccg
    15301 gttgtacgcc gcacatcttc agcgtggcaa aaaagcgcgg cggatgccca atccctgcca
    15361 tcgccaccac atgttcaagc tgagcaacgt cacaacgcgt accggtacgt aaattcaccg
    15421 cctgacccgg cagcagatgc atggggattt caccgctgcg ggggacaccg ccgttgacga
    15481 ttaccgcatc aaccgacttt aagcgccccg ctcgctcacg cattggcccc gccggcaacc
    15541 accagccatt gccaaagcga cgcacaccat caataacgac aatttccaca tcacgcgcca
    15601 gacggtaatg ctgtaaaccg tcgtcggtta cgatgatctg cacatcaggg tgttgcgcca
    15661 gaatggcttt taccgcatca gaacgaacgg gagaaaccgc aacaggcgca tcagtgcgtt
    15721 gataaatcaa cacaggttca tcacccgcct gtgctgttgt ggtatctgcc gacaataaca
    15781 gcggataaga ttcagcctta ccaccatatc cccgcgatac gacccccacg cgaataccgc
    15841 gctgttgcaa ctgttccacc agccagacaa cgaccggggt ttttccgttg ccgcctgcgg
    15901 tgagattacc aaccacgaca accggtacgg gggcacgcca ggcgcgcttc agttttagtt
    15961 tatagcaaag acggatcgcg ccactcacca ggccatacaa ccaggagagt ggcagcaata
    16021 gccgccacaa aggggattca ccagaccaga ttttttcgat cattggccaa actgcatttt
    16081 gtgaagttgc gcgtaaacgc cacggtgctc aagcaaatcg ttatgcgtac cgcgttccac
    16141 aatgacacca tcctcgacga ccacgatttc gtctgccttt tcaatggtag acaagcggtg
    16201 ggcaatcacc agagaggtac ggtttttctg caactcatcc agtgccgcct gaatcgcacg
    16261 ttcggattcg gtatccagag ccgaggtagc ttcgtccaga atcagaatcg ggctatcacg
    16321 caacaaggct cgagcgatag caatacgctg acgctgaccg ccagagagca gcacgccgtt
    16381 ttcaccaatc actgtatcga gaccgttatc catcttattg atgaagtcca tggcgtaggc
    16441 catacgcgcc gcttcttcaa tttgctcacg gctgtactgt tcagtccgtg cgtaagcaat
    16501 gttgttagca accgtatcgt taaacagatg gacattctgc gacaccagag caacctggtt
    16561 acgtaacgac gccagtgtat actcgcgcag atcgtgacca tccatcagga tttcgccttc
    16621 atcaatatcg taaaaacgcg tgatcaggct ggcgatggtt gatttacccg aaccagagcg
    16681 tccaaccaga gcaaccgtct tccctgccgg aattttcagg ttgatgttac gcaatgctgg
    16741 tacgtcacgt cccggataag taaaggtgac attgcggaat tccacgtcgc cagtcgcacg
    16801 ctcgatcacg cgcttacctt cgtctttctc ctgctcactg tccagaatgg taaacagcgt
    16861 ctgacaagcc gccataccgc gctggaactg ggcgttaacg ttggtcagcg atttcagcgg
    16921 acgcatcagt gcaatcattg aagagaaaac aacggtaatc gtaccggcag tcaggctatc
    16981 catgacactt gggaagctcg ccgcatacag aacaaacgcc agcgccaaag aggcgatcag
    17041 ctgaatgatc ggatcagaga tggaagaggc tgaaaccatt ttcatcccct gaagacgcat
    17101 tcggttgctg actttatcga agcgtttcgt ttccacttcc tgaccaccga aaatcaatac
    17161 ttctttatgg cctttcagca tttgttcagc gctggtggtc acctgtccca tggtgttctg
    17221 catgttttta ctgatgttgc gaaaacgctt cgatacaacg cgaatcgcaa tcgaaacaat
    17281 cggtgccagc acaatcaaaa tgatcgacag ttgccaactg taatagaaca tcatgatgaa
    17341 caggccgatg attgacgcac cttcacgcac aaccgttatc agtgcgccgg aagaagaaga
    17401 agcaacctgt tcggaatcgt aggtgatacg tgacaacagc gtccccgttg actgtttgtc
    17461 aaaaaatgaa accggcattc ccatcatgtg accaaacagg cggcgacgca tggtcattac
    17521 cacctttcct gatacccagg agatacagta gctggagaca tagctggtga taccacgtaa
    17581 aatcatcagc ccgatcacca ccagcggcat ccacaccagc acggagcgat ctgttttacc
    17641 aaagccatca tcaagaagtg gcttaaggag cgataacatg aaggtatcgc tggctgcgtt
    17701 gaggattaac gctacgcccg ccacgatcag acccgcttta aaaggcgcaa tggttggcca
    17761 cagtcggcgg aacgtctgcc acgtagagag atctttgtcg ttatgcattc aaaaaaccag
    17821 catttgttga aatagccgca tattctaccc gttatccact ggcacgccaa accactgatg
    17881 ataccaacga ggtaaaattt gctccctgag gctgctaatc cgccagcctt gcgctgaaaa
    17941 attgaccgtt gtttgacctt gatgtggagt atcaatccat tgatatcctt gcagttgata
    18001 gcgatgctta actttgttag agggcagtcg ccatgcgtta tagcgcgatg ccgatgcgag
    18061 tgccactttt ccattcactc gctgaattaa tggcaacgat gatgaggtat tactgccatg
    18121 gtgaggtacc tgaagcaatg ttgcctgcac ttgctgccag taacggctta gcatcttttg
    18181 ttcagctggg gcttcaatat caccggttag aagaatgcta ttcgtcccgt catcaacctt
    18241 aaccacacag gaatggttat ttcctttatc gttgctacct tgtaaaggcc agtgcgcgct
    18301 gaaacgcaat ccttgccatt gccacgcttc gccacgcaca cagggctgat gatgttccca
    18361 gtttaacgga cttctgatcc ataacatcgg ccatatatgc aatattgaat ccagccctcc
    18421 ccggtgatcc agatgttcat ggctcagaat aacgccttcc ggttcaagat tatgccagtg
    18481 gagccagggg ataatcagtt gttgcccact atccccttca ggccaggcca gtcctgtgtc
    18541 atagagaatc gctttgccgt ttctggctat caccattgcc agcccttgcc cgacatcaag
    18601 catgtacagc tgccactcgt caggtcgagg tttttgccac agcggccagc acatcagcaa
    18661 gcctccagcc acacacattg ctggcaacgt tcgccaggcg tttaatcgcc ataccaccag
    18721 taagaaccat ggggaaaatg atagccattg ccaacattca gcaatgttga tccacccttc
    18781 cggcaaactc tttaaccccc agaaaagtaa agccaaagac cggtcggcaa gaaaccataa
    18841 cccttgctcc aggattaacg gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg
    18901 aaccgtgata aatgtcacca agggaattgc taatagattt gcaataaacg aggtcagact
    18961 aatgccatga aatatgacga tttgcacggg cataagcaga agtgtgattc ccagttgcag
    19021 atggatgagg gaaacaactg cacgcaatac cggcggcagt tgccactcag gacagggaaa
    19081 ccactgatac caaaatatca atgccgcgac cgcagcggca gagagccata aactttgcga
    19141 gagaatggca acaggatcca tcagcaaaat tgccgccaga caacatatcc atacatccca
    19201 gccactccac tgtcgcccac ttaacttaag cattccccac gtagcaagcg ccaccatggt
    19261 acgcaatgca ggaggttgca ttcccgtcaa ccaggcataa aaagcagcac agcagattcc
    19321 gccaattaat ggtatttgcc agtggatcca gcgcccaggc agaaaaattt gtccactgcg
    19381 aatgagtccg gcagccagca acgccgcaaa agcgatgtgc aatcccgata tcgccattaa
    19441 atgcgccgtt ccagtatcgc gcatgatatt tttgatttct ttggggaggg ataaccgttc
    19501 ccccatacct aaaccaagaa taaccgcatt ccacggatag ggttgcagcg ttgtttgtag
    19561 tgacgccaga tactgtgcac gcaggctaca attcggttca atgacacttg cctgcagaaa
    19621 acggccggtg agcggctgat gctgggcaat ggcataacgc tggctatcaa agccgccatc
    19681 attaagttgg ccatgaactg cacgaacttt gagtttcatt gaccattgtt gtccagcaca
    19741 aaccgcttgc ggaagatatt cgccatacat cacgagacct gacgcaggga atatacgtcg
    19801 accttgtaga tgagtaattt gaccgtaatg agtggtcatg ccgtcagttg cagtgatctc
    19861 aacaattgca tcctgcgtcg cgccagttaa ggtttctcct gcccacaaaa tttgctttgc
    19921 tgataatatg ccccaaacga aaaacagcaa cgtcagcgcg atataacgga cggttttaac
    19981 agggataaat gccagtacgc aggcgaagag agtcagaaac gcaagggtta atgtcccagg
    20041 caattggggc aaaatcagca acggaaaaat tccgctaatt atgcatacac cgactgtcgt
    20101 tattttcatg cccccctccc tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg
    20161 gtgggaattt tgcgttcccc gaggcatatt cagaagaatt taacgaaagg gtgaaaactg
    20221 ttcaaaactc tgcgaggcag cttccagttc cagattagag aaatgaaaaa agcacccgac
    20281 aggtgctttt ctctcgttca agtttgagta aaaaacttaa ccgtaaatat tggcgcgatc
    20341 gcgcagttct ttaccaggtt taaagtgagg aacgtatttt ccttccagtt ctactttatc
    20401 gccagtcttc ggattacgtc cggtacgtgg tgcgcggtag tgcaaagaga aactgccgaa
    20461 accgcggatt tcaatacgct cgccctgcgc aagagtcgag gccatatgct ccagcatctc
    20521 ttttactgca tcttcaaccg tcttggcggg aatgtgcgat tgctgggtgg caagtctttc
    20581 tatcaattct gacttggtca tgattcctcc ggttccttaa aggcaaatta agcggctgct
    20641 gttgctgcat tgattaaggg cggccgtagg cgcccttagt gcttgattac aggacgaaac
    20701 ctgcaatctg tcaagtaaac tcaacaaact tcggaataaa aatcccgaag agtcagagaa
    20761 ttactcgcct ttagctgctt tgaaagcttc agccattgcg ttgttggaga agtttgcatc
    20821 ttcctgtttg ttaacagttg cgattgcatc tttctcgtca gcttcgtctt tcgcacgaac
    20881 agacaggctg attgcgcggt ttttacgatc aacgccggtg aatttagctt caacttcgtc
    20941 gccaacgctc agaaccaggg tagcgtcttc aacgcggtca cgggatgctt cagaagcacg
    21001 caggtaacct tcaacgccgt cagccagttc tacggttgcg cctttagcgt caactgcagt
    21061 tactttaccg gttacgatag cgcctttctt gttcagagca acccagttgt tgaacggatc
    21121 ttctgcgagc tgtttaacgc ccagggagat acgttcacgt tctgcgtcaa cctgcagaac
    21181 aactgcagcg atttcgtcgc cttttttgta ttcacgaact gcttcttcgc ctgcaacgtt
    21241 ccaggagatg tcagacaggt gaaccaggcc gtcgatgccg ccgtccaggc cgatgaagat
    21301 accgaagtca gtgatagact tgattttacc ttcaacacgg tcgcccttgt tgtgggtttc
    21361 cgcgaactgc tgccacgggt tagctttgca ctgtttcaga cccagggaga tacgacgacg
    21421 ttcttcgtcg atatccagaa ccataacttc cactacatcg ccaacgttaa caactttgga
    21481 cgggtggatg tttttgttgg tccaatccat ttcggaaacg tgtaccaggc cttcaacgcc
    21541 ttcttcgatt tcaacgaagc agccgtagtc ggtcaggttg gtcacgcgac cagtcagttt
    21601 ggtaccttcc ggataacgtt tagcgatagc tacccacgga tcttcgccca gctgtttcag
    21661 gcccagggat acacgggtac gttcgcggtc gaacttcagc actttaacag tgatttcgtc
    21721 gcccacgttg acgatttcgc tcggatgctt aacgcgtttc caggccatgt cagtgatgtg
    21781 cagcaggccg tcaacgccgc ccagatcaac gaatgcaccg tagtcagtga ggttcttaac
    21841 gataccttta acttccatgc cttcctgcag gttttccagc agctgatcgc gctctgcgct
    21901 gttttcggat tcgataacgg cacgacgaga aacaacaacg ttgttgcgct tctgatccag
    21961 cttgattact ttaaattcaa gctctttgcc ttccaggtgc agagtgtcac gcaccggacg
    22021 aacgtctacc agagaacctg gcaggaacgc acgaataccg ttcagctcaa cagtgaagcc
    22081 gcccttaact ttgccgttga taacaccggt aacagtttca gcatcttcgt aagctttttc
    22141 cagcgtgatc caggcttcgt gacgtttagc tttctcacgg gacagcagag tttcaccgaa
    22201 gccgtcttct actgcgtcca gagcaacgtc aacttcgtca cctacctgga tttccagctc
    22261 gccctgggcg tttttgaact gctcagccgg gatggcggac tcagatttca gaccagcgtc
    22321 aaccagtact acgtctttgt cgatagcaac aacaacgcca cgaacgatag aacccgggcg
    22381 ggtttcgatt tcttttaagg actcttcaaa gagttgagca aaagattcag tcatgtttaa
    22441 tcttcaggtt tatatttaac gtccacctgg ctccatgccg gatggggttg tttaacatac
    22501 ccgctgtcat tccattgcaa cgggggtact gcaaattcgg tcgcttatgc gagagccaat
    22561 ttctggcgcg cgtattgtag cgctttttca atcacttgct caatgcttaa ggtggtggaa
    22621 tccaacacta aagcatcggc tgccggaacc agtggcgcta ccgctcggtt acgatcgcgg
    22681 tcgtcgcgtt ctttgatctc ggccaaaagg cgctcaaagt taacactaaa gcccttctcc
    22741 tgcaactgta gcatgcggcg atgcgcacgt tcttccgagg aggcgtcaag gaaaattttc
    22801 actggcgcat cagggaatac caccgttccc atgtcgcggc catcggcaat caggcctggt
    22861 aattcgcgaa acgcgcgttg gcgacgcaat aatgcttcac gaacgcgtgg gaatgccgcg
    22921 acttgtgaag ctgcattcgc cacttcctga gtacgaattt cgccgctgac atcttcccct
    22981 tcgaggatca cttccagatt gccattggtc gacacaaaac gtacatccag atgggatgcc
    23041 agcggcacca gcgcatcttc cgatgcaaca tcaacatggt gatgtaaagc cgccagtgcc
    23101 agtacgcgat aaattgcacc cgagtccagc agatgccatt gcaacgcttc cgccatagcc
    23161 ttacacaagg tgcctttccc tgcaccgctt gggccatcaa tggtaataac cggggcaatt
    23221 gccgtcatct ttatctcctt aaaacaggcg taccgttaac ataaccgcgc gcattatacg
    23281 cgccgacgcc acaaatcgtt aacgttgtgt gcaaaaacca gttttgtctg tattcagatg
    23341 gggatttatt gcgcaattat agcgggctgg cagaagacca gcccgaaaga tgacaatgat
    23401 ttacttacca gaggcgatac gatcacggat gtgttgcgca cgctctgttg atggtgggtg
    23461 agagtcaaac atggactggg tgcgaccgcc atccaggcta gccagttttt caaagctgcc
    23521 aaccagcccc tgggtgctga tgccacgttt cttcaacaga tcaaaggaga aatcatctgc
    23581 ttcggactct ttatcacggg agtatttagc attgatagcg ccttctgcga tatcacctaa
    23641 ttgtgagcgg gaaagctggg aagccacacc gctggtagct gaaatggcat cgcgtgcggc
    23701 aacgatcgca taagaagctt tcatttcagc cagcgagtga cccaacgcga cgtggcccag
    23761 ttcatggccc agaacgcctt caatttcgtt gtcattcatc atgtccatca ggccactgta
    23821 gacacgaaca cagccgttcg ccatcgccca tgcgttgacg tcgctggtca tataaacctt
    23881 atagttgacc ggcgtgccat taatattgtt acccaatgct ttggcgattt ttgccagacg
    23941 tttaccgtac ttgctggatt tacttgcgac ttgattgccg ctgtccattt cggcacagcc
    24001 ctgattggca atcgcttttg catcggcatc gcttaatgtt gctgctttat aggcatttaa
    24061 accggagctg atagccatat tggtatcaat accgtgggta ttttgacacc ctgtcagtaa
    24121 tgctgcagag gtcgca
//
LOCUS       DBBGLQ010000063        23704 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.063, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000063 DBBGLQ010000000
VERSION     DBBGLQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.063"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(329..1465)
                     /locus_tag="ACIYSB_003441"
     CDS             complement(329..1465)
                     /locus_tag="ACIYSB_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGC5430420.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1468..1830)
                     /locus_tag="ACIYSB_003442"
     CDS             complement(1468..1830)
                     /locus_tag="ACIYSB_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGC5430421.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2367..4280
                     /gene="mtlA"
                     /locus_tag="ACIYSB_003443"
     CDS             2367..4280
                     /gene="mtlA"
                     /locus_tag="ACIYSB_003443"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGC5430422.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4510..5658
                     /gene="mtlD"
                     /locus_tag="ACIYSB_003444"
     CDS             4510..5658
                     /gene="mtlD"
                     /locus_tag="ACIYSB_003444"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGC5430423.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5658..6245
                     /gene="mtlR"
                     /locus_tag="ACIYSB_003445"
     CDS             5658..6245
                     /gene="mtlR"
                     /locus_tag="ACIYSB_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGC5430424.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6257..6466)
                     /gene="yibT"
                     /locus_tag="ACIYSB_003446"
     CDS             complement(6257..6466)
                     /gene="yibT"
                     /locus_tag="ACIYSB_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGC5430425.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6751..7113
                     /locus_tag="ACIYSB_003447"
     CDS             6751..7113
                     /locus_tag="ACIYSB_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGC5430426.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            7487..9142
                     /gene="lldP"
                     /locus_tag="ACIYSB_003448"
     CDS             7487..9142
                     /gene="lldP"
                     /locus_tag="ACIYSB_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGC5430427.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            9142..9918
                     /gene="lldR"
                     /locus_tag="ACIYSB_003449"
     CDS             9142..9918
                     /gene="lldR"
                     /locus_tag="ACIYSB_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGC5430428.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            9915..11105
                     /gene="lldD"
                     /locus_tag="ACIYSB_003450"
     CDS             9915..11105
                     /gene="lldD"
                     /locus_tag="ACIYSB_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGC5430429.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            11303..11776
                     /gene="trmL"
                     /locus_tag="ACIYSB_003451"
     CDS             11303..11776
                     /gene="trmL"
                     /locus_tag="ACIYSB_003451"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGC5430430.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(11829..12650)
                     /gene="cysE"
                     /locus_tag="ACIYSB_003452"
     CDS             complement(11829..12650)
                     /gene="cysE"
                     /locus_tag="ACIYSB_003452"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGC5430431.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(12730..13749)
                     /gene="gpsA"
                     /locus_tag="ACIYSB_003453"
     CDS             complement(12730..13749)
                     /gene="gpsA"
                     /locus_tag="ACIYSB_003453"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGC5430432.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QENIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(13749..14216)
                     /gene="secB"
                     /locus_tag="ACIYSB_003454"
     CDS             complement(13749..14216)
                     /gene="secB"
                     /locus_tag="ACIYSB_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGC5430433.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(14279..14530)
                     /gene="grxC"
                     /locus_tag="ACIYSB_003455"
     CDS             complement(14279..14530)
                     /gene="grxC"
                     /locus_tag="ACIYSB_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGC5430434.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(14672..15103)
                     /locus_tag="ACIYSB_003456"
     CDS             complement(14672..15103)
                     /locus_tag="ACIYSB_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGC5430435.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            15348..16892
                     /gene="gpmM"
                     /locus_tag="ACIYSB_003457"
                     /gene_synonym="pgmI"
     CDS             15348..16892
                     /gene="gpmM"
                     /locus_tag="ACIYSB_003457"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGC5430436.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            16926..18185
                     /gene="envC"
                     /locus_tag="ACIYSB_003458"
     CDS             16926..18185
                     /gene="envC"
                     /locus_tag="ACIYSB_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGC5430437.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            18189..19148
                     /locus_tag="ACIYSB_003459"
     CDS             18189..19148
                     /locus_tag="ACIYSB_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGC5430438.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPATVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(19135..20169)
                     /gene="waaH"
                     /locus_tag="ACIYSB_003460"
     CDS             complement(19135..20169)
                     /gene="waaH"
                     /locus_tag="ACIYSB_003460"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGC5430439.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(20408..21433)
                     /gene="tdh"
                     /locus_tag="ACIYSB_003461"
     CDS             complement(20408..21433)
                     /gene="tdh"
                     /locus_tag="ACIYSB_003461"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGC5430440.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLAAIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(21443..22639)
                     /gene="kbl"
                     /locus_tag="ACIYSB_003462"
     CDS             complement(21443..22639)
                     /gene="kbl"
                     /locus_tag="ACIYSB_003462"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGC5430441.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(22914..>23704)
                     /gene="yibB"
                     /locus_tag="ACIYSB_003463"
     CDS             complement(22914..>23704)
                     /gene="yibB"
                     /locus_tag="ACIYSB_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HGC5430442.1"
                     /translation="GFREKLARSVDVYFSYFERLAALENEMIIFTSPDLKPRVEAIRN
                     GKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNPEYWSPEYVLVCNLKA
                     YFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHLFTIKKGLTVT
                     SQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMC
                     YYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         5838 a         6203 c         6040 g         5623 t
ORIGIN      
        1 ttgtgatcta tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc
       61 atcatcgcaa tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg
      121 aacaactcat atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa
      181 gacggataaa aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt
      241 aaagaaaaat catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag
      301 caaagttcca taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc
      361 tggtcattct tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa
      421 ccgccacctg ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt
      481 caatggttcc cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct
      541 gcgcctgata agaaccgcct ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca
      601 cctgcccagg tagcgcgtta aacaccactt ccgcatcatc gccgggtttc agacgtaaca
      661 gcgagttttg ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aagaccatca
      721 ccggacgcag cggcaaggca gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt
      781 agccattgct cggcgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt
      841 gcgctcttaa gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct
      901 ggatctgcgc ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt
      961 gccgcgcatc gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca
     1021 agtaacgttg ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg
     1081 cctgcgcttc agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt
     1141 cagcctgaag tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct
     1201 cgcccttttg aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct
     1261 gtggcgtgat agggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt
     1321 agttcatcaa caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca
     1381 gcgtccactg atttaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt
     1441 aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt
     1501 tttctcggcg gcgagttgat gctccagccg ggaaattcgg tcagaaagtg cggcgatttc
     1561 cgggtcagtc gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg
     1621 ttgatagagc gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct
     1681 cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt tggcaatcag
     1741 ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag
     1801 cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt
     1861 gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg
     1921 cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct tagttacata
     1981 acgattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa
     2041 atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacacta
     2101 ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg
     2161 ttttgtgatg aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc
     2221 acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac
     2281 aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga
     2341 cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct
     2401 ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta
     2461 tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg
     2521 tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg
     2581 taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg
     2641 cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta
     2701 agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata
     2761 acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc
     2821 cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg
     2881 acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg
     2941 ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat
     3001 caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca
     3061 tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc
     3121 tggggggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg
     3181 cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg
     3241 tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt
     3301 acttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta
     3361 ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta
     3421 tgcaggacat gaaagctgag tctaaaggcg catctccgct gtctgctggc gatgtgacta
     3481 acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttccagtg
     3541 cgatgggcgc aggcgttctg cgtaagaaaa ttcaggatgc aggtctgtcg cagatttctg
     3601 ttactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg
     3661 acctgaccga acgcgctatg cgccaggttc cgcaggcaca gcatatttcg ctgaccaact
     3721 tcctcgacag cggcctgtac accagcctga ccgaacgtct ggttgctgcc caacgccaca
     3781 cggcaaacga agagaaagta aaagacagcc tgaaagacag ctttgacgat tccagtgcta
     3841 acctgttcaa gctaggcgcg gagaacatct tcctcggtcg caaagcggca accaaagaag
     3901 aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggaatacg
     3961 ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg
     4021 tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtc gtgttctgcc
     4081 agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt ctggtgattg
     4141 gtattgctgc ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg
     4201 atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac
     4261 tgctggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg tggggttaat
     4321 tgcctgatgc gctacgctta tcaggcctac aggatgcatc acaatttgtt gaatttgcac
     4381 gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc tcctcacgga
     4441 gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag
     4501 gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt
     4561 aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt
     4621 gatgccctga atgcccgtca tagctatcag gtacatgtgg ttggtgaaac cgagcaggta
     4681 gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt tgatctgatt
     4741 gctcaggttg atttagtcac taccgccgtt ggcccggttg tgctggaacg tattgctccg
     4801 gcaatcgcca aagggctggt gaaacgtaaa gaacaaggta atgaatcccc gctgaacatc
     4861 atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt gatgaacgcc
     4921 ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt
     4981 gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt gacggtagaa
     5041 accttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc gaacatccca
     5101 ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac
     5161 acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac cattcgtgac
     5221 gcgattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgca
     5281 gtattgatca agcgctacgg ctttgacgct gacaagcatg cggcgtacat ccagaaaatt
     5341 ctcggccgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagcca
     5401 ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac tgctcggtac gctggaatat
     5461 ggtctgccac ataagaacct gattgaaggt attgccgctg caatgcactt ccgcagtgaa
     5521 gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc gcaggcggcg
     5581 ctggcacaga tttccggtct tgatgccaac agcgaggttg tatccgaggc ggtaaccgct
     5641 tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat
     5701 tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc
     5761 gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag ctcctgaccg
     5821 aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg
     5881 ctgtagaacc gttactcgac ggcgatggtc cgctgggcga tctttctgtg cgtttaaaac
     5941 tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggaa ctgctgatgg
     6001 cattgcgtga agagctaaat cacgacggca acgagtacgc ctttaccgac gacgaaatcc
     6061 ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac
     6121 cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa
     6181 caatggtcct ttcactgact gagctgattt ccaaaatcag cttaaaaaaa gcctttcaaa
     6241 agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat
     6301 actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt
     6361 tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta
     6421 cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt
     6481 attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg gtaaagatga
     6541 cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt atcttttttc
     6601 tatagatttc ttaattaatc aacgaattgc gtagtttttg agcatgttaa tgattacgtt
     6661 acagaactat tacaatgtca ttagttgtga acaaagcacc tggtcgcgca tactaggggc
     6721 tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa
     6781 cgtctcagcg atcgcctgga cgccatccgc caccagcagg ccgatctttc gctggttgaa
     6841 gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct
     6901 cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg
     6961 ccattccagc gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt
     7021 gttcgcggac tggtcgttgt gcacccaatg accgcactgg gccgcgaaat gggcctgcag
     7081 gagatgactg ggttttcaaa gaccgcgttt taagaacaca gtatctacag ggtgattctg
     7141 cacattccta taggccgagt aaggtgttca cgccgcatcc ggcaagataa ggcgctctgg
     7201 atcaacaatc taagggcaat tctctgatga ggattgccct tttctttacc agacatctcc
     7261 cttcaccaaa aaattggccc taccaattct tcgcttatct gacctctggt tcacaattcc
     7321 ccaattaaaa ctcacatcaa cgttgccaat acataacatt tagttaacta ttcattgtca
     7381 ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac aaggagatga
     7441 gcaacagact cattacacga tgtgcgtgga ctccaggaga cctgcaatga atctctggca
     7501 acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat cgcttcccat
     7561 cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg ccgcctcatg
     7621 gacggtggca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc
     7681 gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt ggatcattat
     7741 tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca tcattcgctc
     7801 gtctattctt tcgataaccc cagaccagcg cctgcaaatg ctgatcgtcg gtttctgttt
     7861 cggcgcgttc ctcgaaggag ctgcaggctt tggcgcaccg gtagcaatta ccgccgcatt
     7921 gctggtcggt ctgggcttta aaccgctgta cgccgccggg ctgtgcctga ttgttaacac
     7981 cgcgccagtg gcatttggtg cgatgggcat tccaatcctg gttgccggac aggtaacagg
     8041 catcgacagc tttgagattg gccagatggt ggggcgtcag ctaccgttta tgaccatcat
     8101 cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaaag aaacctggcc
     8161 tgcggtcgtg gttgcgggcg gctcgtttgc catcgctcag taccttagct ctaacttcat
     8221 tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc tgacgctgtt
     8281 cctcaaacgc tggcagccag tgcgcgtatt ccgttttggt gatttggggg cgtcacaggt
     8341 tgatatgacg ctggcccaca ccggttacac tgcgggtcag gtgttacgtg cctggacacc
     8401 attcctgttc ctgacagcta ccgtaacact gtggagtatc ccgccgttta aagccctgtt
     8461 cgcatcgggt ggcgcgctgt atgagtgggt gatcaacatt ccggtgccgt acctcgataa
     8521 actggttgcc cgtatgccgc cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa
     8581 gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc tctcgattgt
     8641 ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc tgaaagaact
     8701 ggctctgccc atctactcca tcggtatggt gctggcattc gcctttattt cgaactattc
     8761 cggactgtca tcaaccctgg cgctggcact ggcgcacacc ggtcatgcat tcaccttctt
     8821 ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct catctaacgc
     8881 cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg atctgttgct
     8941 ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc aatctatcgc
     9001 tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc gctttactgt
     9061 caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc aggcttatgt
     9121 cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga cgaggttgcc
     9181 gatcgtgtgc gggcgctgat tgatgaaaaa aacctggagg cgggcatgaa gttgcccgct
     9241 gaacgtcaac tggcgatgca actcggcgta tcgcgtaatt cactgcgcga ggcactggca
     9301 aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg
     9361 cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct gatggccgat
     9421 gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc cagcaccgca
     9481 tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct ttgctttgaa
     9541 gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg
     9601 attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt cttcgatgtc
     9661 ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc ggttttttca
     9721 caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga tgccgacggg
     9781 gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa acgattcgat
     9841 gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa tgagcattcg
     9901 agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca gcgcaacgca
     9961 ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa tacacgctgc
    10021 gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt
    10081 ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg gtagcactgg
    10141 caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg
    10201 cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg attgaagaag
    10261 tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc gatcgcggct
    10321 ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg
    10381 tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg agcggcccga
    10441 atgcggcaat gcgccgttac ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg
    10501 gtctgaacgg tcgtccgcat gatttaggta atatctcagc ttatctcggc aaaccgaccg
    10561 gactggaaga ttacatcggc tggctgggga ataacttcga tccgtctatc tcatggaaag
    10621 accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg atcctcgatc
    10681 cggaagatgc gcgcgatgcc gtacgttttg gtgctgatgg aattgtggtt tctaaccacg
    10741 gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct attgcagatg
    10801 cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg cttgatgtcg
    10861 tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc ttgtatgcgc
    10921 tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa aaagagatga
    10981 aagtggcgat gacgctgact ggcgcgaaat cgatcagcga aattacgcaa gattcgctgg
    11041 tgcaggggct gggtaaagag ttgcctgcgg cactggctcc catggcgaaa gggaatgcgg
    11101 catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg accgttcggc
    11161 gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc ctcgcccctc
    11221 cggggagagg gccggggtga ggggaaaagg ccgcactgct caccaatttt ctgctatcct
    11281 gcccccgcac taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc
    11341 aaatactggc aacatcatcc gtctttgcgc taataccggc tttcgtctgc atatcatcga
    11401 accgatggga tttgcctggg acgataagcg cctgcgccgc gcggggctgg actatcacga
    11461 gtttaccgcc gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca
    11521 gcgcctgttc gccctcacca cgaaaggtac acctgctcac agcgccgtaa gctatcagga
    11581 tggcgattat ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc
    11641 cctgcccgct gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct
    11701 gtccaatgcg gtgtcggtag tggtgtatga agcctggcgg cagttggggt atccgggagc
    11761 ggtattgaga gattagttac tgtaggccgg atagatgatt acatcgcatc cggcacgatc
    11821 acaggacatt agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg
    11881 tccatatcca ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca
    11941 gcggcggtgg tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg
    12001 ccgcgcccaa cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca
    12061 cgaattttcg ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc
    12121 agaatcgata cgtcgttttc aatcaccgcc gtttcaccaa cgacgatgcc tgtcgcgtgg
    12181 tcaagcatga taccgcgacc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca
    12241 gaaacctggt tttgcagaaa gattgccagt gcgcgacgcc cctgattcca caaccagtga
    12301 ccgatgcgat aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat
    12361 ttatcgactg ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc
    12421 atttccgggt cagcggcgta ggcttcttcc accacttcac ggatagcaat agcaggcata
    12481 attggcgatg acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc
    12541 ttgagtagcg tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg
    12601 gcttcggctt taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc
    12661 acacgatgag ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt
    12721 tccctggggt tagtggctgc tgcgctcgtc cttgcgtgca cgacctagta aagtcaatgc
    12781 tgcctcgcgc gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat
    12841 ttcaacgccg aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac
    12901 cacctgacca atattctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc
    12961 aaaacggcgg ttacgcgact ggttgtcggt acaggtaagc accagatcgc caagccccgc
    13021 catgcccata aaggtggcag ggtcggcacc cagcgccgca ccaagacgcg acatttcagc
    13081 cagcccacgg gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcggacat
    13141 ccccgcacca atggcaataa cgtttttcac cgcgccgcca agctgcacgc caatgaaatc
    13201 cggattgctg taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc
    13261 aaaggtctga tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt
    13321 cgcaaacgtt gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc
    13381 cacgtcctgt aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg
    13441 cgcatcagga cgcatcagtg gtttaatctg gcgcagcact tcaccaaaga catggctggg
    13501 tacgacgacg agaatattac ggctggctgc cagcgcagtg gcgagatcgc tttcaagatg
    13561 gagcgtatcg ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag
    13621 cgttgcgata tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag
    13681 ggtgatggca agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg
    13741 ttggttcatc aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag
    13801 ttcatgaaca gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg
    13861 gataccatgc tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgct
    13921 cccaggcaat gcgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc
    13981 tgaacttcac acaggaacgc ggtttcttcg cccaaagagg ccgttacggt aacacgcagt
    14041 accacttcgt atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact
    14101 tctggttgcc aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc
    14161 ttggtataaa tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt
    14221 agaaaaccct taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt
    14281 atttcagcag gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccac
    14341 caatgtgctg tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca
    14401 tctcttcacg cttggcggcg ttgccatcga tcggcagctc ctggaaactc acgcccttgc
    14461 tgctcagcag tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa
    14521 cattggccat aaattagctc ctgttttttt accctgaaat ttcatgttgc agggcggcag
    14581 caaaatggag tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta
    14641 acggcctgca tcatgaaaga cgacaggtaa attatttgcc gcgcaccaaa ggcaagtttt
    14701 cgccagccca gccagcgacg ccttctttca gcacgaatac ttgcgcaaaa ccagctttcg
    14761 tcagtgcgtt tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccggtt
    14821 tgtctttgtg cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat
    14881 taatagaacc tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca
    14941 cagcgtcttc tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg
    15001 aggtcaggct cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac
    15061 tcagtatggg atgacggcca acaaattgca taatttcttg catggggggt aacaactccc
    15121 gacgtagtga ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc
    15181 agcgcgttaa ctggaatgca attttgcggg gcgcgacgaa aaaaaagcgt aaatctggca
    15241 tcggcttgcc gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct
    15301 gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta
    15361 aaaaacctat ggtactggtg attctggatg gctatggcta tcgcgaagaa cagcaggata
    15421 acgccatttt tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata
    15481 ccctaatcga cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg
    15541 aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg
    15601 acgttgaaat caaagatcgc gctttctttg ctaatccggt gctgactggc gcggtagata
    15661 aagcgaaaaa cgcaggcaaa gcggtacaca ttatgggtct gctctccgca ggcggcgtac
    15721 acagccacga agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa
    15781 aaatctacct gcacgcattc cttgacggtc gcgacactcc gccgcgcagt gctgaatcct
    15841 cgctgaaaaa attcgaagaa aaatttgccg cgctgggcaa aggccgcgta gcgtccatca
    15901 ttggtcgtta ctacgcgatg gaccgcgata accgttggga tcgcgtagaa aaagcttatg
    15961 acctgctgac tctggcgcag ggcgagttcc aggccgatac cgccgttgct ggtttgcagg
    16021 ctgcttatgc tcgcgacgaa aatgatgaat tcgtgaaagc gaccgttatc cgtgctgaag
    16081 gtcagccaga cgcggcaatg gaagacggcg atgcactgat tttcatgaac ttccgtgctg
    16141 accgcgcgcg tgaaatcact cgtgctttcg tgaacgctga tttcgatggc ttcgcgcgta
    16201 agaaagtggt taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg
    16261 cggttgctta cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg
    16321 acaaaactca gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca
    16381 acggtggcgt agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag
    16441 tggctaccta cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg
    16501 cggccatcaa gagcggcaaa tacgacacca tcatctgtaa ctatccgaac ggcgacatgg
    16561 taggtcacac cggggtgatg gaagcggcgg ttaaagcggt tgaagcgctg gatcactgcg
    16621 tggaagaagt cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc
    16681 acggtaacgc tgagcagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca
    16741 acctgccagt tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gaaggcggca
    16801 aactttctga catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga
    16861 tgactggtaa gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta
    16921 ataccatgac acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca
    16981 gcgttcttag cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc
    17041 aactcaaatc tattcaggct gacatcgccg caaaagagcg cgcggtacgc caaaagcaac
    17101 aacaacgcgc aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca
    17161 cccgtaagct gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga
    17221 tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg
    17281 cacaactgga tgccgcattc cgtcagggcg agcataccgg tattcagctg attctcagcg
    17341 gtgaagaaag ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc
    17401 gacaagaaac cattgcccag ttgaagcaaa cgcgtgaaga agtcgccatg cagcgtgctg
    17461 aactggaaga gaaacagagc gagcaacaaa cgctgttata tgagcagcgc gcccaacagg
    17521 cgaaactgac tcaggcgctg aacgagcgta aaaagacgct ggcagggctg gagtcttcca
    17581 tccagcaagg tcagcaacag ttgagcgagc tgcgcgccaa cgaatcccgt ctgcgtaaca
    17641 gcattgcccg tgcggaagcc gcggcgaaag cgcgtgcaga acgagaagca cgtgaggccc
    17701 aggcggttcg cgaccgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg
    17761 aaagcgaaaa atcgctgatg tcccgtaccg gtggtctggg cgcgccgcgt ggtcaggcat
    17821 tctggccggt tcgcgggccg acgctgcatc gctatggcga acagctacag ggtgaattac
    17881 gctggaaagg tatggttatc ggtgcttctg aaggtactga agttaaagcg attgccgacg
    17941 gtcgggtgat tctggctgac tggctgcaag gctacggtct ggtggtggtg gttgagcatg
    18001 gtaaaggcga catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc
    18061 aggttcgcgc gggccagccg attgcactgg tgggcagcag tggcggtcag ggtcggcctt
    18121 cactctattt cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa
    18181 gataagtttt gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct
    18241 cctcccccgt acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc
    18301 acaacgaaaa ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt
    18361 caccgcacgc cagagagatg gcgaccaaag cgcataacag cgggcacgaa gtgttgattc
    18421 atctcccgat ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga
    18481 tgagcagcga cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg
    18541 tggggatcaa caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag
    18601 tgatgcaggc gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata
    18661 cccaggcgat gcgcgccgcg caaggcactg gcgtgaaggt gatcaaacgg aaggtattcc
    18721 tcgacgattc gcaaaatgaa gcggacatcc gtgtgcaatt taatcgcgca attgacctgg
    18781 cgcgtcgcaa cggttcgacc attgccattg ggcatcctca ccctgcaacg gtacgcgtgt
    18841 tgcaacagat ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc
    18901 tgaatgaacc gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc
    18961 gtaatccgtt ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg
    19021 ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact
    19081 tccagcatca gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg
    19141 caaattaaag gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca
    19201 taagcggaaa gaccacagta acgcctggta acccaccttc acgctgcgca cattggtaat
    19261 caggcgctta tacataccgg aagtaaatat ctcggcaatc atccgttggc gggtaagaat
    19321 atccggctct ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta tttgctgatg
    19381 aaattccgga taaatcgtaa ttttgtcggc ataatttcga tttaatttct ccagcaggcg
    19441 ggtaatctta atatagtgac gttgataatt aaggttttta ttcccttgtc tatgcaaccg
    19501 actcactgac gtattatgca gataatattt atataatgat tgctcggtat atcgcgcacg
    19561 cagcgcatta aacatgaatt ctgttgtcca gacaatatcc tgatgatgta atccggcaat
    19621 aaatttaatg ttatttttaa caataacatc gcggcgataa acccccatcc agacaacgtg
    19681 agtccaacgg cgcgaagaaa gccccatccg cagccagtcc gggccggtta atacaccggt
    19741 tgagcgaagg cgatcgctgg ggatagattg ccaggtttct cccgtttcac gaaaacacca
    19801 gtcagcgttg cactgcgcta catcgaggtc gtcctctaac gccatggtca tcagcgtttc
    19861 gtacatggtg ggatagactt catcgtcagc atcgacaaaa gcgacatatt tgcccgttgc
    19921 cacctcgatc ccacgattac gcgccaccga tgcgccagca ttcgcctgat gcaacaaacg
    19981 aacgtgcgga tagttttctg cgtaatgctt tgctatttca acagaattat ccgttgaacc
    20041 atcgttaata ataatgattt ccagagcagt ccaggtttgc gtaattaaag attccataca
    20101 agtgcggaaa tcatcgcccg cattatataa cggaataata acactaagtt tattggtgct
    20161 gttcatcata aattaccgac gacctctgaa gttatcctga cgttttacag gctttgtatt
    20221 aagaaactat taagcctgtg agatattatt tttacaggtg agagagtcac ttcagggtag
    20281 agagataatg agagcagcta tataagagat cagcgcaatt acgatatctt tattacactg
    20341 acaggatttt acatatttaa cggattatcc tcaggcaaaa gggctggaat accagccctt
    20401 tttcgtgtta atcccagctc agaataactt tcccggactg gcccgaacgc atggcgtcaa
    20461 agcccttctg gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatcgaggc
    20521 cagactgaat cagcgccgcc atcttgtacc aggtttcaaa catctcacga ccgtaaatgc
    20581 ctttaatgaa caagccttta aagatcactt tggtccagtc gatagacatg tcagacggtg
    20641 gaatacccag catcgcaata cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg
    20701 ctggcggcgc gccggacatt tccagaccga catcaaagcc ttcggtcatg cccagttcag
    20761 ccatcacatc attaaggttt tctttcgcga cgttaaccgc acgggtgata cccattttgc
    20821 gcgccagctc aaggcggtat tcgttaacat cggtgatcac cacattgcgt gcaccaacgt
    20881 gtttcgccac cgccgcagcc ataataccta tcggacctgc accagaaacc agcacatctt
    20941 cgcccaccag atcaaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaattg
    21001 cggctaagtc atcggaaata ttgtcgggga ttttgaaggc gttgaatgcc gggatcacca
    21061 gatattcggc aaagcagccc gggcgattaa caccaacgcc tatcgtgttg cggcacaaat
    21121 gggtacgacc accacgacag ttgcggcaat gaccacaggt gatatggcct tcgccagaaa
    21181 cgcgatcgcc gatcttgaag cctttcactt cctgaccaat acctaccact tcaccgacat
    21241 attcatggcc cacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat
    21301 agatgtgaac gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat
    21361 gcccgagttc cggtacagga acgtcggtca tccagatgcc ctcttccgct ttcagtttgg
    21421 ataacgcttt catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtaa
    21481 atgcttctac tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa
    21541 tacgcgcctg acctttcgga acgaccggat agaagaaacc ggtaacgtaa atgccctctt
    21601 tttgcagctc acgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa
    21661 tggcgtgatc ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac
    21721 gcgcgttcgc ccacagacgg tcacgcagtt cgctgcccgc ttcgaccatc tccagtactt
    21781 tgatggacgc ggcaacaatg gccggtgcca gcgagttgga gaacaggtac ggacgagaac
    21841 gctggcgcag ccactcaacc acttctttgc gcgccgcggt ataaccacca gaagccccgc
    21901 ccagcgcttt accaagcgta ccggtgataa tatcgacccg gcccatcaca tcgcagtatt
    21961 catgggaacc acgaccattt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca
    22021 ccagggcatc atatttatct gccagatcgc aaacgccctt caggttggca atcacgccgt
    22081 ccattgagaa cacaccatcg gtggcgatca gcacatgacg cgcaccggct tcacgcgctt
    22141 ctttcagacg tgcttccagc tcctgcatat cgttgttggc atagcgatag cgtttagctt
    22201 tgcacagacg cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt
    22261 cttccgcacc cagaagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga
    22321 gaatcgcatc ttccatcccc aggaaggccg ccagtttttg ttcaagctct ttatggctgt
    22381 cctgagtgcc gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg
    22441 cctttgccgc cgcaatcaga tcaggatgat tcgccagccc gagatagttg ttggcacaaa
    22501 agttaatgac gtggcttcca tcagccacag tgatatctgc ttgctgcgca gacgtaataa
    22561 tgcgctcttc tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct
    22621 gataaaattc tccacgcatt gcgattctcc agacagggca aattccagca catattaccc
    22681 aaacttatag gtcgcgacga gataacgcgt taacacttct gcaaaattca ggataaataa
    22741 cgtgtaactt gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg
    22801 ggcataatga tgattgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat
    22861 catttttcct atctcagttg gtatcaaaac gctacccact aatggaggga aacctatttt
    22921 cttgaaagga aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag
    22981 cgaaacaaat cgaaccattt tcctctcccc agatagttta ggttgaagag atcggggcgt
    23041 ttgtaatagc acatgacaaa aatgccctgg tcatcgtcca caatgttatt atttagtgtt
    23101 attttttgac tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca
    23161 attgcgcccc caataatata tacatgattg ccaatcatga aatcaaaaac ctgttgttga
    23221 gatgtgacag taagcccttt ttttatagtg aaaagatgca tcttactttc atcaaatggg
    23281 aaatcccaaa ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc
    23341 cacgcaacaa gaggcgtttt cactaagccc atgttgatag ctttatttac aaaataggcc
    23401 ttaaggttac ataccaaaac atactctggt gaccagtact ctgggttttt taattgtcga
    23461 ggctcaagtc tatttgtaaa cgactcatct ttttgaattt tttcgatacg acttctgata
    23521 tatctaaatt tcttttttat atcgataacg ataaccgttg ttggttttcc gttgcgaatc
    23581 gcttcaaccc ttggtttcaa atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg
    23641 gccagacgct caaagtaact aaaataaaca tcaactgaac gagctagttt ttcacggaac
    23701 cctt
//
LOCUS       DBBGLQ010000064        22870 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.064, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000064 DBBGLQ010000000
VERSION     DBBGLQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.064"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(139..420)
                     /gene="yehE"
                     /locus_tag="ACIYSB_003464"
     CDS             complement(139..420)
                     /gene="yehE"
                     /locus_tag="ACIYSB_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HGC5430443.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(683..1792)
                     /gene="apbC"
                     /locus_tag="ACIYSB_003465"
     CDS             complement(683..1792)
                     /gene="apbC"
                     /locus_tag="ACIYSB_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGC5430444.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1924..3957
                     /gene="metG"
                     /locus_tag="ACIYSB_003466"
     CDS             1924..3957
                     /gene="metG"
                     /locus_tag="ACIYSB_003466"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGC5430445.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPSNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4098..7892
                     /locus_tag="ACIYSB_003467"
                     /pseudo
     CDS             4098..7892
                     /locus_tag="ACIYSB_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7902..11534
                     /gene="yehI"
                     /locus_tag="ACIYSB_003468"
     CDS             7902..11534
                     /gene="yehI"
                     /locus_tag="ACIYSB_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGC5430446.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQPQSVLKSYLSAPPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATEPARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLFVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSHYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVAHVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTNDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYNLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11595..11912
                     /gene="yehK"
                     /locus_tag="ACIYSB_003469"
     CDS             11595..11912
                     /gene="yehK"
                     /locus_tag="ACIYSB_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGC5430447.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            12219..13307
                     /gene="yehL"
                     /locus_tag="ACIYSB_003470"
     CDS             12219..13307
                     /gene="yehL"
                     /locus_tag="ACIYSB_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGC5430448.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVECIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13318..15597
                     /gene="yehM"
                     /locus_tag="ACIYSB_003471"
     CDS             13318..15597
                     /gene="yehM"
                     /locus_tag="ACIYSB_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HGC5430449.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDASGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQKIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQKAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTFAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCATIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     EADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            15590..16726
                     /gene="yehP"
                     /locus_tag="ACIYSB_003472"
     CDS             15590..16726
                     /gene="yehP"
                     /locus_tag="ACIYSB_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGC5430450.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVCQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            16723..18723
                     /gene="yehQ"
                     /locus_tag="ACIYSB_003473"
     CDS             16723..18723
                     /gene="yehQ"
                     /locus_tag="ACIYSB_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGC5430451.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQTLWLGGISQPLIHYEAAFNRALQAAETCNWRWVSESLRQLRASVDA
                     FHARASHYNAGECLHQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     TPVAPLPSASVLPVPSTAHQLLIRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            18848..19309
                     /gene="yehR"
                     /locus_tag="ACIYSB_003474"
     CDS             18848..19309
                     /gene="yehR"
                     /locus_tag="ACIYSB_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGC5430452.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(19349..19819)
                     /gene="yehS"
                     /locus_tag="ACIYSB_003475"
     CDS             complement(19349..19819)
                     /gene="yehS"
                     /locus_tag="ACIYSB_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGC5430453.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(19866..20585)
                     /gene="btsR"
                     /locus_tag="ACIYSB_003476"
                     /gene_synonym="yehT"
     CDS             complement(19866..20585)
                     /gene="btsR"
                     /locus_tag="ACIYSB_003476"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGC5430454.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(20582..22267)
                     /gene="btsS"
                     /locus_tag="ACIYSB_003477"
     CDS             complement(20582..22267)
                     /gene="btsS"
                     /locus_tag="ACIYSB_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGC5430455.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            22489..22554
                     /locus_tag="ACIYSB_003478"
                     /pseudo
     CDS             22489..22554
                     /locus_tag="ACIYSB_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22771..>22870
                     /locus_tag="ACIYSB_003479"
     CDS             22771..>22870
                     /locus_tag="ACIYSB_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HGC5430456.1"
                     /translation="MRVEICIAKEKFSKMPKGSVPALQQEMSRRIRK"
BASE COUNT         5784 a         5896 c         5741 g         5449 t
ORIGIN      
        1 aagagtatta actatttatc gcatctatca attaatgtag atttatgtaa atggtattgt
       61 tgatatcaat aaaaaagccg aaatcattta tataaatgat ttcggctttc taaatgcaat
      121 taaaatcagg ttgccaaatt aatcgtagcg atttaacacg attcgacgtt tgctatcgct
      181 acctgcaaca accacttctg tcgttacacc tttgacgggc gaagaaaaca gatgacgggt
      241 gtcaacatta tacgttaact gaccacaatg ctgttgaggt tgaccattaa ccatagccat
      301 actgcatgtt gggggactga tcctgccatt aatcgctaat gtggcagtct ctgaagagaa
      361 tgcgggagaa gccaatccat aggcaagaaa aataattcct gacagccaat acttattcat
      421 tgaacgttat ccctgtagta aaggttatgc ctggatagaa tgagtgcata acaaactata
      481 gctgtacatc cactacacag ccacgaagga tgataatgaa gcattgcctg tatgatcaat
      541 cgactttgta gagtttcgga cgaaggtccg cagaatattc gcagtattaa ataagtgttc
      601 attaatgcgg caaaaactaa tacaccgcat caatgtaaca tctctgcggt gtatgaagaa
      661 taaccagaat ggttaatagg cgttagaccg cgcggaagga aatctcgcct ggaatgactt
      721 caccctgcca gtagagctga gctgcaacgc ggtcagccag ctggcggtag atagcggtaa
      781 attcgctctc cggacggctg ataaccgttg gcgtaccttt atcgagatcc tcacgtaagg
      841 agatgtgtaa cggcatctgc cccagcagct gtgtatggta tttttcagcc agtttctctg
      901 caccaccggt accgaaaatc ggttcatggt gaccacagtt actgcaaata tgcacactca
      961 tgttctcgac aataccgagc accggaacct cgactttctc gaacatcaca atgccttttt
     1021 tcgcatcgat cagcgcgatg tcctgcgggg tggtgaccac gactgcaccg gttactggaa
     1081 tgttttgcgc cagcgtcaat tgaatgtcac cggtgcctgg cggcatatca agcaccagat
     1141 agtcgagatc cggccacaag gtttcctgca acatctgcat taacgccttg ctcgccatcg
     1201 gtccacgcca caccatcgca ttgtcgtcag tgaccagata accaattgaa ttggttgcca
     1261 ggccatgaga cataatcggt gccatatggg taccgtccgg cgaagttgga cgctgatttt
     1321 ctgcgcccaa cattgttggg attgatgggc cgtagatatc ggcgtccaga atgccgactt
     1381 tcgccccttc tgccgccagc gccagtgcca ggtttaccgc cgtggacgat ttacccacgc
     1441 cgcctttacc ggaactcacc gcgataatgt tcttcacacc gttaattccc ggctgattct
     1501 tcacgcgttt cagagtggct atgttatgcg acagtttcca gtcgatagct ttcgccccgg
     1561 tgatgcgcag cagttcggcg ctacattgct cttttaactc ttcgaacgcg ctatgccaga
     1621 caaacggcat aaccagttcc acatgcaacg tgtcgtccat ccaggcgaca tggtgcaacg
     1681 ctttcagcgt ggtgaggtta tgcttcaggg ttgggtgctg aaaattggcc agtgtcccgg
     1741 cgaccattgc gcgcaaggct tctggtgatt tggcctggga ttgttcgttc atcccgactc
     1801 cttttttgta tagataaacc atcagctgat agtttacctg aagaatatag agaagtactt
     1861 acttaacatt ttcccatttg gtactatcta accccttttc actattaaga agtaatgcct
     1921 actatgactc aagtcgcgaa gaaaattctg gtgacgtgcg cactgccgta cgctaacggc
     1981 tcaatccacc tcggccatat gctggagcac atccaggctg atgtctgggt ccgttaccag
     2041 cgaatgcgcg gccacgaggt caacttcatc tgcgccgacg atgcccacgg tacaccgatc
     2101 atgctgaaag ctcagcagct tggtatcacc ccggagcaga tgattggcga aatgagtcag
     2161 gagcatcaga ctgatttcgc aggctttaac atcagctatg acaactatca ctcgacgcac
     2221 agcgaagaga accgccagtt gtcagaactt atctactctc gcctgaaaga aaacggtttt
     2281 attaaaaacc gcaccatctc tcagctgtac gatccggaaa aaggcatgtt cctgccggac
     2341 cgttttgtga aaggcacctg cccgaaatgt aaatccccgg atcaatacgg cgataactgc
     2401 gaagtctgcg gcgcgaccta cagcccgact gaactgatcg agccgaaatc ggtggtttct
     2461 ggcgctacgc cggtaatgcg tgattctgaa cacttcttct ttgatctgcc ctctttcagc
     2521 gaaatgttgc aggcatggac ccgcagcggt gcgttgcagg agcaggtggc aaataaaatg
     2581 caggagtggt ttgaatctgg cctgcaacag tgggatatct cccgcgacgc cccttacttc
     2641 ggttttgaaa ttccgaacgc gccgggcaaa tatttctacg tctggctgga cgcaccgatt
     2701 ggctacatgg gttctttcaa gaatctgtgc gacaagcgcg gcgacagcgt aagcttcgat
     2761 gaatactgga agaaagactc caccgccgag ctgtaccact tcatcggtaa agatattgtt
     2821 tacttccaca gcctgttctg gcctgccatg ctggaaggca gcaacttccg caagccgtcc
     2881 aacctgtttg ttcatggcta tgtgacggtg aacggcgcaa agatgtccaa gtctcgcggc
     2941 acctttatta aagccagcac ctggctgaat cattttgacg cagacagcct gcgttactac
     3001 tacactgcga aactctcttc gcgcattgat gatatcgatc tcaacctgga agatttcgtt
     3061 cagcgtgtga atgccgatat cgttaacaaa gtggttaacc tggcctcccg taatgcgggc
     3121 tttatcaaca agcgttttga cggcgtgctg gcaagcgaac tggctgaccc gcagttgtac
     3181 aaaaccttca ctgatgccgc tgaagtgatt ggtgaagcgt gggaaagccg tgaatttggt
     3241 aaagccgtgc gcgaaatcat ggcgctggct gatctggcta accgctatgt cgatgaacag
     3301 gctccgtggg tggtggcgaa acaggaaggc cgcgatgccg acctgcaggc aatttgctca
     3361 atgggcatca acctgttccg cgtgctgatg acttacctga agccggtact gccgaaactg
     3421 accgagcgtg cagaagcatt cctcaatacg gaactgacct gggatggtat ccagcaaccg
     3481 ctgctgggcc acaaagtgaa tccgttcaag gcgctgtata accgcatcga tatgaggcag
     3541 gttgaagcac tggtggaagc ctctaaagaa gaagtaaaag ccgctgccgc gccggtaact
     3601 ggcccgctgg cagatgatcc gattcaggaa accatcacct ttgacgactt cgctaaagtt
     3661 gacctgcgcg tggcgctgat tgaaaacgca gagtttgttg aaggttctga caaactgctg
     3721 cgcctgacgc tggatctcgg cggtgaaaaa cgcaatgtct tctccggtat tcgttctgct
     3781 tacccggatc cgcaggcact gattggtcgt cacaccatta tggtggctaa cctggcacca
     3841 cgtaaaatgc gcttcggtat ctctgaaggc atggtgatgg ctgccggtcc tggcgggaaa
     3901 gatattttcc tgctaagccc ggatgccggt gctaaaccgg gtcatcaggt gaaataatcc
     3961 cccttcaagg cgctgcatcg acagcgcctt ttctttataa attcctaaag ttgttttctt
     4021 gcgattttgt ctctctctaa cccgcataaa tactggtagc atctgcattc aactggataa
     4081 aattacaggg atgcagaatg agacacttta tctatcagga cgaaaaatca cataaattca
     4141 gggcagttga gcaacaggga aacgagttgc atatcagttg gggaaaagtt ggcaccaaag
     4201 ggcaaagcca gataaaaagt ttttcagatg ctgcggcagc ggcaaaagcg gagcttaagc
     4261 tgattgcgga gaaggtgaag aaggggtatg tggagcaagc gaaggataat tctttgcaac
     4321 cttcccaaac ggtaacgggc tctctcaagg tagcggactt atccaccatt attcaggaac
     4381 aaccctcttt cgtagcagaa acccgtgcgc ctgacaaaaa tacagatgct gttttaccgt
     4441 ggctggcgaa agatattgct gtcgtttttc cgcccgaagt tgtacacacc acgttaagtc
     4501 atcgccgctt tcccggagtt cctgttcagc aagcagacaa attgccccaa ttacgtcgct
     4561 tagcctgtag tgtgtcgcaa cgggataata aaacagccac atttgacttc agcgcctgtt
     4621 ctttagaatg gcaaaacacc gtcgcccagg cgatcagtca gatcgacggc ctgaaaacaa
     4681 cacagttacc atcaccagta atggctgtac tcacggcact tgaaatgaaa tgcacaagat
     4741 ataaagtgcg tgaggatgtt atggatcaga tcgtccagga aggcggtctg gaatatgcta
     4801 ctgatgtaat aatacacctt caacagattg atattgaatg ggattatgcg aataatgtca
     4861 ttattattct gccgtctggc attgcaccta gctacttgga gcaatattcc agatttgaat
     4921 gacgcctacg taaacattta tcactgacgg aagagtctct ctggcaaaaa tgtgcacaaa
     4981 aacttattgc cgcaattcca catattccag aatggcggca accattaatt gctttgttat
     5041 tacccgaaaa accagaaatt gcacatgaaa ttgcccagcg tctactgggg caaaaaaaat
     5101 taccctcgct tgagtggtta aaaatagtgg cgactgatga gcacattctt gcctcattag
     5161 aaaaatatca cgaaccatat gccatttttg atgattacta ttgtggtgcg atatggtcag
     5221 ccaccgtatt acaggagcaa ggtgttgcag ccctgccccg atttgctccc tatgccgcaa
     5281 gtgactactg cgccgatgtg ttgcgtcata tcaatcatcc gttcgcattg acactgctta
     5341 tacgtgtagc cgggcaaact aaacgctgtc acgatcggat gacgaaagcc attgctgcgt
     5401 tcccacatgc agcaatggcg gcactgacgg aacttcttgg gcaaaaagaa gagaacagtt
     5461 ggcgcattat gctaatgaca atgcttatct cacaaccagc actggcagaa caggtcattc
     5521 cctggctctc gacacccgca gttgccgtac tgaaatcatg ccagcaacaa ctgacacagc
     5581 cctcaaacca tgccagcgcc gatctactgc cagccgtagt agtctcccct ccctggcttt
     5641 cgaaaaagaa aaaatcgccg attccggtgc tggatttagc gccattaggc attgagccaa
     5701 tctgttatct gacagaagaa atcagtaatc aacttttggc gaaatatatc tggtattcaa
     5761 aacacatcac ggttagccat gaagaaagta ctaccaacct gttggcaagg atgggttttc
     5821 aacgacggat cgctggtaca tatattaaag ctcccgaagc ggtagttgag gcatggctaa
     5881 atgaagatta ttcaacctta ctaagtgaat ttaaggtgtt tcattcacct accgggcatt
     5941 attggcagtt ggggattttg acaacattgc cgctggagaa agcagtaaaa gcatggaatg
     6001 cccttaccct atctccacat accgataccg aatactccat gttacatttt ggactcaaag
     6061 ggttacctgg gttagtaaac tcacttgcac gctatccaca agaagccttg cccatcacga
     6121 attacttcgc agcgagtgag ctggctcctg ccgtcgcccg tgccttcaac aaactgaaaa
     6181 cgctacgcga aaacgcccgt agctggctgt tgaaataccc ggaacatgcc cttaccggcc
     6241 tgctgcctgc ggcgctcggc aaagccggtg aagcacagga taacgcccgc gctgccttgc
     6301 gtatgcttac cgaaaacggt catcagccat tactgcaaga aatcgcccga cgttataacc
     6361 agccggaagt aaccgatgcg gtgaacgctc tgcttgcgct cgatccctta gataatcacc
     6421 cgacaaaaat ccccactctt ccggcctttt atcagccatc gctctggacg cgcccggtat
     6481 taaaagcaaa tgcccaatca ctgccagata gcgccctcct ccacctcggt gaaatgctcc
     6541 gcttccctca ggaagaggct ctgtatccgg gattattgca ggtgaaagac gtctgttccg
     6601 ccgactcact ggcgggattt gcctgggatc tgtttaccgc ctggcagacc gctggcgcgc
     6661 cgtcgaaaga gagttgggcg ttcactgcgt taggcgttct cggtaacgat gacaccgccc
     6721 gcaaactgac gccattaata cgcgcctggc ctggtgaatc ccagcataaa cgcgccaccg
     6781 ttgggttgga tattctcgct gctatcggta gtgatatcgc ccttatgcag cttaacggca
     6841 tcgcccagaa actgaaattc aaagcattac aggagcgggc aaaagaaaaa attgccgaca
     6901 ttgccgagag ccgcgaactc acggtggcgg agcttgaaga tcggttagca ccggatctcg
     6961 gtctggatga taacggttcg ctgctgctgg attttggccc acggcagttc accgtcagct
     7021 ttgatgaaac cttaaaaccg tttgtgcgtg atgtttccgg cagccgcctg aaagacctgc
     7081 ccaaaccgaa caaaagcgat gatgaaacgc gggcgaacga tgcggttaac cgctacaaat
     7141 tgctgaaaaa agatgcgcgt accatcgccg cccagcaggt agcaaggctg gaatccgcca
     7201 tgtgcctgcg ccgccgctgg tcgctggaaa acttccagct cttcctggtt gagcatccgc
     7261 tggttcgtca cttaacccgc cgtctgattt ggggcgttta tagcgccgaa aaccagctac
     7321 tggcttgctt tcgcgtagca gaagataaca gctccagcac cgctgacgat gatcttttca
     7381 ccctgccgga aggcgatatc tctatcggca ctcctcacgt tctggaaata tcaccaacgg
     7441 atgctgccgc ctttggtcag ctttttgccg actacgaact gctaccaccg ttccgccagc
     7501 tcgaccgtaa cagctacgcc ctgacagaag ccgagcgcaa tgccagtgaa ctgacccgct
     7561 gggcaggcag aaaatgcccg agtggtcggg tcatggggct ggcgaataaa ggctggataa
     7621 agggcgaacc acaggatgga ggctggatcg gatggatgat caaacctttg ggtcgctggt
     7681 cgttaatcat ggaaatcgat gaaggctttg cggtaggcat gtcgccagcc gaactcagcg
     7741 ctgagcagct cttaagcaag ctgtggctat gggaaggcaa agcagaaaga tatggctggg
     7801 ggagtaattc aacacaggaa gcgcagttct ccgtaatcga tgccatcacc gccagcgagc
     7861 taattaacga tattgaagcc ctgtttgaat aaggaaagag catggacaag gaattaccgt
     7921 ggctggcgga taacgcccaa ctggaactga aatataaaaa aggcaaaacg ccgctcagtc
     7981 atcgtcgctg gccgggcgaa ccagtgtccg ttatcactgg aagtctcatc cagacattgg
     8041 gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat tgtctggcgt tatgagaatt
     8101 tttcactgga gtggcagtcc gccatcacgc aggccatcaa cttgatcggc gaacacaaac
     8161 cctcaatccc ggcccggaca atggcggcgc tagcctgtat cgcgcaaaat gacagccaac
     8221 agttgctcga cgaaatcgtc caacaagagg ggctggaata tgcgactgag gtggtgattg
     8281 cacgccagtt tattgcgcgg tgttatgaga gtgatcctct ggtagtgaca ttgcagtatc
     8341 aggacgagga ttatggctat ggttatcgct cagaaaccta taacgaattc gatctccgac
     8401 tgcgtaagca tctctctctg gcagaggaaa gctgctggca gcgttgcgcc gacaaactca
     8461 ttgccgcact accaggaata accaaagttc gccgcccttt tattgcgctg atcctcccgg
     8521 aaaaaccaga aatagccaat gagttggtag gccttgaatg cccgcgaact cattttcatt
     8581 ctaaggagtg gttaaaagtt gttgctaatg accccacagc ggtgagaaaa ctcgaacact
     8641 actggagcca ggatatattt agcgatcgag aagccagcta catgtcgcat gaaaaccact
     8701 tcggctacgc ggcctgcgcc gcccttttgc gcgaacaagg actggcagcc attccgcgcc
     8761 tcgcgatgta tgcccataaa gaagattgcg gcagtctgct ggtacaaatt aaccatccgc
     8821 aagtcatccg caccttgcta ctggtggctg ataaaaacaa acccagcctg caacgtgtag
     8881 ctaaatacca taaaaacttc ccccatgcga cgctcgccgc actggcagaa ctgctggcgt
     8941 taacagaacc accagcccgc cctggttatc caatcatcga agacaaaaag ctgcctgcac
     9001 agcaaaaagc acgcgatgaa tactggcgta cgctgttaca aacgctgatg gcatcgcagc
     9061 cacaactggc agcagaagtg atgccgtggt taagtactca accccagtca gtgctgaaga
     9121 gttatttatc ggcaccgccc aaaccggtta ttgatggcac cgataacagc aatctgccag
     9181 aaatcctcgt ttcaccaccg tggcgtagta agaaaaaaat gacagctcca cgtcttgatt
     9241 tggcaccgct cgaattaact ccgcaagttt actggcaacc aggcgaacaa gagaggcttg
     9301 ccgccactga gcctgcccgt tatttcagca cggaatctct tgcgcaacgc atggaacaaa
     9361 aaagtggacg agttgtatta caggaactgg gttttgggga tgatgtatgg ctgtttctga
     9421 attatatact ccccggaaaa ctggatgctg cacgcaattc actctttgtt cagtggcatt
     9481 actaccaggg gcgggttgaa gagatcctga atggctggaa ctccccggaa gcacaattag
     9541 cagaacaggc gctccgcagc ggtcacatag aagcgttaat taacatatgg gaaaatgaca
     9601 actactcaca ttatcgtccg gaaaagagtg tctggaacct gtatttattg gcacagttgc
     9661 cgcgtgagat ggctttgacc ttctggctgc gtatcaatga gaaaaagcat ctgttcgcgg
     9721 gtgaggacta ttttctcagt atcctcggat tggatgcgct accaggtctg ctgttggctt
     9781 tttcacatcg tccaaaagaa acatttccgt taattttaaa ttttggcgca acagaactgg
     9841 cgctgcctgt tgcccacgtc tggcgacgtt tcgcggcgca gcgtgatctg gctcgccagt
     9901 ggattttaca atggccggaa catacggcta gtgcacttat ccctcttgtc tttaccaaac
     9961 ccagcgataa tagcgaagcc gcattacttg ccctgcgttt actgtacgaa cagggacatg
    10021 gcgaattgct acaaaccgtg gcaaaccgct ggcagcgtac agatgtatgg tctgccctgg
    10081 agcagttgct taaacagggt ccaatggaca tttacccggc acgcattcca aaagcccctg
    10141 atttctggca tccgcaaatg tggtccaggc cgcgccttat cactaataat caaactgtta
    10201 ccaatgacgc tctggaaatt atcggcgaaa tgctgcgctt tacccagggg ggacgttttt
    10261 atagcgggct ggaacaactg aaaacgttct gccagccaca aacgctggca gcttttgcct
    10321 gggatctctt cactgcgtgg caacaagctg gtgcccccgc aaaagacaac tgggcatttc
    10381 tggcgttaag tctctttggt gacgaaagca cggcacggga tctgacgaca cagatcctcg
    10441 cctggccaca agaaggcaaa tctgcccgtg ctgtcagcgg cctgaacatc cttaccctga
    10501 tgaataatga tatggcgctg atacagctgc atcatatatc gcaacgggct aaatcccgcc
    10561 ccttacgtga taacgcggcg gaatttcttc aggtggtcgc agaaaatcgc gggctaagcc
    10621 aggaagagct agcggacaga ttagtcccaa ccctgggcct tgatgatccg caggcgttga
    10681 gttttgattt tggtccccgg cagtttaccg ttcgcttcga tgaaaatctc aacccggtta
    10741 tctttgatca gcaaaacgtt cgccagaaaa gcgttccccg tttgcgcgcc gatgacgatc
    10801 aactgaaagc gcccgaggca ctggcccgac taaaagggct aaaaaaagat gctactcagg
    10861 tgagcaaaaa cctgctcccg cgtcttgaaa cggccctacg taccacccga cgctggtcgc
    10921 tggcagattt tcattctctg tttgttaatc atccctttac ccgtctggtt acccagcgat
    10981 taatatgggg ggtttatccg gcaaatgaac cgcgttgttt actcaaagcc tttcgtgtgg
    11041 ccgcagaggg ggagttctgc aatgcgcaag atgagccaat tgacctgcct gcggacgctc
    11101 tgattggcat tgcccacccg ttagaaatga cagcagaaat gcgcagtgaa tttgcacagc
    11161 tttttgccga ttacgaaatt atgccgcctt ttcgccagtt gtcgcgccgc acggtgctgc
    11221 tcacacctga cgagtcaacc agtaacagcc tgactcgctg ggaaggtaaa tccgctaccg
    11281 ttgggcaact tatgggaatg cgatacaaag gctgggagtc aggctatgag gacgcatttg
    11341 tctataacct gggtgagtac cggctggtcc ttaagttttc acccggtttt aaccactaca
    11401 atgttgatag caaagcgcta atgagcttcc gttctcttcg agtgtaccgt gacaataaat
    11461 ccgtcacttt tgccgaactt gatgtgtttg atttgagtga ggcgttaagc gcacctgacg
    11521 tcattttcca ttaaaacaca gcgggcagtg atgcaactgc ccgttatcaa cacgacttac
    11581 cagcggggaa agcgatgatc gtgcaaaaag agctggttgc tatttacgat tatgaggtcc
    11641 ctgtacctga agatccgttt tccttcagac ttgagatcca taaatgctct gaattattta
    11701 caggttccgt ctatcgactg gagcgattcc ggctacgtcc aacatttcat caacgtgatc
    11761 gagaagatgc tgacccgcta ataaatgatg cgttgattta tataagagat gagtgtattg
    11821 atgagcggaa attacgaggt gaatcacctg aaactgtaat agcaattttt aatcgtgaac
    11881 tacagaatat attcaaccaa gaaatagaat aatatactct aaataattca aattggtccg
    11941 atccggcgca acgtcccaat ggcctggatt ataaatctca ttatcttaat tgcaacgggg
    12001 tccagccgtg gttataattc gttcgttgga acacgatgtt gcacaggctg tggtgttagg
    12061 cctgaaaata gtaaataaaa gaacctgcct caccagcagg tttttttatt tactgtgatc
    12121 tgctttccag atatttttcg ctcaaacaac taatgcgcca aacatttatt gcgcgtaaaa
    12181 tatcgtttat ttcattaata catttcaggg atgaatatat gtcaccgcag aataatcatc
    12241 tgcagcgtcc gcctgctgct gtgttatacg ccgatgaact ggcaaaatta aaacaaaatg
    12301 ataacgcacc ttgcccgccc ggttggcagt taagtttgcc tgcggcccgt gcttttatcc
    12361 ttggcgacga agcgcaaaat atcagccgta aagtggtgat tagcccctcc gctgtcgaac
    12421 gtatgttagt cactcttgct accggccgcg gtttgatgtt ggtgggggaa ccgggtaccg
    12481 caaaatctct tctttctgaa ttactggcaa ccgctattag cggcgatgcc gggttaacca
    12541 tccagggcgg ggcatctacc accgaagatc aaatcaagta tggctggaac tacgccctgc
    12601 ttatcaatca tggtccgtca acggaagcgc tggtccctgc cccgctttat cagggaatgc
    12661 gcgacggcaa aatcgtccgt ttcgaagaga taacgcgtac accgctggaa gtgcaggact
    12721 gtctgttagg tatgctttct gacagggtga tgacggtgcc ggaactcact ggtgaagcca
    12781 gtcagctcta tgcgcgagaa ggctttaata ttattgccac tgccaatacc cgcgatcgcg
    12841 gcgtcaatga aatgagtgct gcgctaaagc gtcgcttcga ttttgaaacg gtttttccga
    12901 ttatggattt cgcccaggag ttggaactgg tcgccagtgc ttcggcgcgt ttgctggccc
    12961 atagcggtat tccgcataaa gtgcctgacg cggtactgga attactggtc cgcaccttcc
    13021 gcgatctgcg tgccaacggc gaaaagaaaa cgtcaatgga tacactgaca gcgattatgt
    13081 ccaccgctga agccgtgaac gtggcccatg ctgtgggcgt ccgcgcctgg tttttagcga
    13141 atcgcgcggg ggagcctgcc gacctggtgg aatgtattgc cggaaccatc gtcaaagata
    13201 acgaagaaga tcgcgcgcgt ctgcgccgtt acttcgaaca gcgtgtcgcc acacataaag
    13261 aagctcactg gcaggcttat tatcaagccc gccaccgcct gccgtgagga aagatgcatg
    13321 agcgagccgt taattgtcgg catccggcat catagtccgg cctgcgcccg gctggtgaaa
    13381 tcgttaatcg aaagccagcg gccacgatac gtgttgattg aaggcccggc tgattttaat
    13441 gaccgggtag acgaactgtt tttagcccac cagcttccgg tagctattta cagttattgc
    13501 cagtatcagg acggtgcagc ccccgggcgt ggtgcctgga cgccatttgc tgaattttcg
    13561 ccggagtggc aggcgctaca agccgcacgt cgcattcagg cacaaactta cttcatcgat
    13621 ttgccttgct gggcgcagag tgaagaagag gacgattcgc ctgatacgca agatgaaagc
    13681 caggccttac tgctgcgtgc cacccgcatg gataacagcg ataccctgtg ggatcacttg
    13741 ttcgaagatg aaagccagca aactgcatta ccctctgcgc tggcgcacta ttttgcccaa
    13801 ctgcggggcg acgcctccgg cgatgcgctc aatcgtcagc gcgaagcctt tatggcccgc
    13861 tggattggat gggcgatgca gcaaaataat ggcgacgtgt tagttgtctg cggtggctgg
    13921 cacgctccgg cactggcaaa gatgtggcgc gaatgccctc agaaaattaa caagccagaa
    13981 ttgccctcgc tggcagatgc cgttacaggt tgttatctca caccctacag tgaaaagcgc
    14041 cttgatgtgc tggcaggata cctttcagga atgcctgccc cggtatggca aaactggtgc
    14101 tggcagtggg gcttgcagaa ggccggtgaa caactgctaa aaactatcct tacccgtttg
    14161 cgccagcaca aattgcccgc ttctaccgcg gatatggctg ccgctcatct gcatgcgatg
    14221 gcgctggcac agttgcgcgg tcatacacta ccgttacgca ctgactggct ggatgccata
    14281 gcaggctcgc tgattaaaga agccctgaac gcgccgttgc cgtggagcta tcgcggcgtt
    14341 attcatcccg ataccgatcc gattctgcta acgttgatag acacattcgc gggtgacgga
    14401 ttcggtaaac ttgccccttc tacgccacaa ccgcctctgc caaaagatgt cacctgcgaa
    14461 ctggaacgta ccgcaatctc ccttccggcg gagcttacct taaatcgctt tacccccgat
    14521 gggctggcgc aaagtcaggt gttacatcgg ctggcaatac tggagatccc tgggattgta
    14581 cgccagcagg gaagtacact gacacttgca ggcaacggtg aagaacgctg gaaattaacc
    14641 cgcccgctta gccagcatgc ggcattgatt gaggccgcct gttttggtgc cacactccag
    14701 gaagccgcac gcaataaatt agaagccgat atgctggacg cgggcggaat cggcagtatc
    14761 accacatgtc ttagccaggc ggcgttagcg ggtctggcgt ccttcagtca acaattactg
    14821 gagcaactca cactattaat cgcccaggaa aatcaatttg ccgaaatggg ccaggcgctg
    14881 gaagtgcttt atgccttatg gcggctggat gaaattagcg gtatgcaagg cgcgcagata
    14941 ttacaaacga cgttatgcgc gactatcgat cgcacgctgt ggctgtgtga atctaacggc
    15001 agaccggatg aaaaggagtt tcacgctcac ctgcatagct ggcaagcgct ttgccatatt
    15061 ctgcgcgatc tacatagcgg cgttaattta cccggcgttt ctctttctgc ggcggtagcc
    15121 ttactggagc gacgcagtca ggcaattcat gccccggcgc tggatcgcgg cgcggctctt
    15181 ggcgcactaa tgcgtctgga acatcccaac gccagtgccg aagcggcgct gacgatgctg
    15241 gcgcagttat ccccggcaca atctggtgag gcgctgcacg gtttgctggc gctggcccgc
    15301 catcaactgg cctgtcagcc ggcatttatc gccggtttca gcagtcattt aaatcaactg
    15361 agtgaagccg attttattaa cgccctgccc gatttacgcg cggcgatggc ctggctacca
    15421 ccacgagaac gcgggacgct ggcgcatcag gtgcttgagc attatcaact ggcgcaactt
    15481 cccgtttcgg cgctgcaaat gccgttgcat tgtccaccac aggccattgc acatcatcaa
    15541 caactcgaac agcaggcact ggcatcgctg caaaactggg gagttttcca tgtctgaact
    15601 gaacgatctt ctgaccaccc gtgagctaca acgctggcga ttaattcttg gcgaagcggc
    15661 agaaacgacg ctttgtgggc tggatgacaa cgcccggcag atagaccacg cgctggagtg
    15721 gctgtatggg cgcgatcctg aacggctcca gcgtggtgaa cgttccggtg gattaggtgg
    15781 ctcaaatctc accacccctg agtggatcaa cagtattcac acgctgtttc cgcagcaggt
    15841 gattgagcgg ctggaaagcg atgccgtgct gcgctacggc attgaagatg tggtgacgaa
    15901 tctcgacgtg ctggaacgta tgcagccttc tgaaagcctg ctacgcgctg ttttgcacac
    15961 caaacatctg atgaaccccg aagtactggc tgccgcccgc cggatagtgt gccaggttgt
    16021 tgaagaaatt atggctcgac tggcaaagga agttcgtcag gctttttctg gtgtccgcga
    16081 tcgccgtcgc cgttcattta ttccactggc gcgaaacttt gatttcaaaa gtactctgcg
    16141 cgccaacctg caacactggc acccgcaaca cggcaagttg tatatcgaat ccccccgctt
    16201 taacagccgc attaaacgcc aaagcgaaca atggcaactg gtcttactgg ttgatcaaag
    16261 cggatcgatg gtcgattcgg tgatccactc tgcggtgatg gcggcctgtt tgtggcagtt
    16321 acccggcatt cgtacccatc tggtggcgtt tgacacaagc gtcgttgatc tcacggcaga
    16381 cgttgccgat ccggtagagt tattaatgaa agtacagttg ggcggcggga ccaatatcgc
    16441 cagtgccgtg gagtatggtc ggcaacttat tgaacaacca gcgaaaagcg tcattatcct
    16501 cgtgagcgat ttttacgaag ggggttcatc atcattactg acgcatcagg tgaaaaagtg
    16561 tgtccagagc ggcatcaaag tgctgggact ggcagcgctc gatagcaccg caacaccttg
    16621 ctatgaccgc gatacggccc aggcgctggt taatgtcggc gcacaaatag ccgccatgac
    16681 gccgggcgag ctggcatcat ggcttgcgga gaatcttcag tcatgaattc actacgtccg
    16741 gaattattag aactgacacc gcaggccctg acggcgttaa gcaatgccgg ttttgttaag
    16801 cgcagtctta aggaactgga aaatggcaac gtcccggaga tcagccatga gaacgacgct
    16861 ttaatcgcca ccttcagtga cggtgtccgt acccagctgg cgaacggcca ggcactgaaa
    16921 gaggctcagt gcagttgcgg ggccaacggt atgtgccgtc atcgcgtgat gctggtgtta
    16981 agttatcaac gactttgtgc caccactcag tctacggaaa aagaagaaga gtgggatccg
    17041 gcaatctggc tggaagaact ggctaccctt cccgatgcta cccgcaaacg cgcacaggcg
    17101 ctggtcgcta aaggcatcac cattgagttg ttctgtgcgc cgggtgaaat tccctctgcc
    17161 cgcttaccga tgagcgatgt gcgtttttat tcccgcagca gtattcgttt cgcccgttgt
    17221 gattgtattg aaggcacact ttgcgaacat gtcgtactgg cggtacaggc cttcgtcgag
    17281 gccaaagcgc agcaagcaga atttaaccat ttaatctggc agatgcgcag cgaacacgtc
    17341 acatcatctg acgatccgtt tgccagcgaa gaaggcaacg cgtgtcgtca atatgttcag
    17401 caattaagcc agacattatg gcttggcggc atcagccagc cgctcatcca ttacgaggca
    17461 gcattcaacc gcgcattgca ggcggcagag acctgcaact ggcgctgggt gagtgaatcg
    17521 ctacggcaac tgcgcgccag cgttgatgcc ttccacgccc gcgccagcca ctataatgcc
    17581 ggagaatgct tacatcagct tgcggcatta aacagtcgat taaattgcgc acaagagatg
    17641 gcccggcgcg acagtattgg tgaagttcct cctgtgccgt ggcgcacggt cgttggctct
    17701 ggcattgccg gagaagcaaa gcttgatcat ctgcggctgg tgtctttagg tatgcgttgc
    17761 tggcaggata ttgagcatta tggtttacgc atctggttta ccgatcccga caccggcagt
    17821 attttgcacc tttcgcgcag ttggccgcga agtgaacagg aaaactcacc ggcagctacg
    17881 cgtcggctgt ttagttttca ggctggcgca ctggcgggcg ggcaaattgt ttcacaagca
    17941 gcaaaacgca gtgccgatgg cgagctgctg ttagctaccc gcaaccgctt aagcagcgtt
    18001 gtgccgctgt cgcctgatgc ctggcaaatg ttgagcgcgc cgttacgcca gccgggcatt
    18061 gtggctttgc gggaatattt acgccagcgt ccccccgcct gcatacggcc tcttaatcag
    18121 gtcgataact tatttattct gccggtcgct gagtgtattt cgctcggttg ggacagcagc
    18181 cgccagacgc tggatgcgca ggtcattagc ggcgaagggg aagataatgt gctgacgtta
    18241 tcattaccag cctcagccag cgcaccttat gccgttgaac gcatggcggc gcttttgcaa
    18301 caaacagacg accccgtgtg tctggtttct ggctttgtca gttttgttga agggcaattg
    18361 acactggaac cacgggtgat gatgacaaaa acccgtgcct gggcgctgga cgcagaaact
    18421 acgcctgtgg caccgctacc ttctgccagc gttttgcctg tgccgtctac tgctcatcag
    18481 ttgctgatac gctgccaggc gttacttatt caactgctcc ataacggctg gcgctatcag
    18541 gaacagagtg ctattggtca ggcagagttg ctggcgaatg acctcaccgc ggtgggtttt
    18601 tatcggctgg cacatgtgtt gggacaattt cgtaatacag aaagcgaggc acgggtagaa
    18661 gcaatgaata acggtgtttt gctttgcgaa caattattcc ccatgcttca gcaacaagga
    18721 tgaaatagtg ctttttacta agagttctac tccagttccg gactgctcac gccacggtat
    18781 taggcatatc ctatatagcc cctggtgaga gtcaccagtt ccttgattaa ataaaatgga
    18841 gttttacatg aaggctttca ataagctgtt ttccctcgtt gttgcatctg ttctggtttt
    18901 ctctcttgct ggctgcggtg acaaagaaga atcgaagaaa ttcagcgcca atctgaacgg
    18961 cactgaaatt gccattacct atgtctacaa aggtgacaag gtgcttaagc aatcttctga
    19021 aaccaaaatt caatttgcct ccattggtgc aaccaccaaa gaagatgctg ccaagacact
    19081 tgagccgtta agcgccaaat acaaaaacat cgcgggtgtt gaagaaaaat taacctatac
    19141 cgatacctac gcgcaggaaa acgtgactat cgatatggaa aaagtggatt ttaaagccct
    19201 gcagggtatt tcaggaatca acgtttctgc tgaagatgcc aaaaaaggta tcactatggc
    19261 gcaaatggaa ctggtgatga aagccgctgg ttttaaagaa gtgaaataat cggttggcgg
    19321 tcatgctcta aacatgaccg ccaatttttt agcctttttt cacatgctgg cgcgctgcca
    19381 gtccacgcag aaaataacgt aaaaattgat cgccgcattc gcggaagttt ttatgatccg
    19441 gtgcgcgcat catcgctgta atttccggca tcgaaacgcg gaactgctgt tcggtgagga
    19501 tagccagaat gtcatcggtt ttcagcgaaa acgcgatgcg taattttttc agcacgatgt
    19561 tgttattaat gcgacgttcc ggctccagtg ccggagcaga ctcatccttg ccgcgttttt
    19621 cataaatcag gccattgagg aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct
    19681 cttcgtcttc tttacgtagc cagacggcga tctgttccgc ggtggcttcg acattaccca
    19741 gcgccagaat acgcaccagg tcattattat tggctttcaa aatgtagcgc acgctgcgca
    19801 gaatatcgtt acttagcatg aggccttcag gtgttgatga ggcaaaaagc cattttagca
    19861 gtcttttaca ggccaatcgc ctcttttaag cttttcagat aacggcggct gaccggcacg
    19921 gttaagccat tacgcaaaat caactcggcc tggccgttat cttccagacg aatctcctgt
    19981 aaatgcgcga ggttaaccag atactgacga tggcagcgca gtagtggtgt acgactttcc
    20041 agggtacgta atgtcaattc ggtaaagccc tctttccctt cgtggctggt aacgtagaca
    20101 ccgctcatcc gactgctgac aaatgccaca tctttcattt gcagcaaata aatccgacta
    20161 tgccccgtac aagggataaa tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc
    20221 tgcttgctgc gctcctgacg caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt
    20281 ggcttcagca gataatcaaa ggcatgttct tcaaaggctt taattgcgta ttcgtcaaac
    20341 gcagtgagaa aaacaatata cgggcgatgt tccgggtcaa gcatccccac catttccaga
    20401 ccactgatgc gcggcatctg gatatcgaga aacagcacat ccgggcgcag tttatgcacc
    20461 gcgccgatcc cttccacggc gtttgaacac tctccaacga tttcaatatc gctctgctcc
    20521 tgcaaaaata cacgcaggtt ctcccgtgct aacggttcat catcgacaat taagacttta
    20581 atcatgcctc gtccctccat ggtagtcgta acgttattcg ggtgtaacta tcaggctcac
    20641 aggcgacgct tattccatag tcatcgccaa accgttcacg taaacgctta tccaccagat
    20701 tcatccccag cccactggca ttggttaccg gttgatacaa accggcattg tcttcgatct
    20761 ccagcatcaa atgttgcccc tcacgtcggg cgctgattgc cactcgccct gtatccagca
    20821 gttgtgatgt cccatgttta atggcgtttt ccactatcgg ttgcagggta aacgcgggca
    20881 attgctgctg ggataattct tgcggaatag caatgttgac ctgcaaccgc gactggaagc
    20941 gcgccttttc aatttgcaga taagcattca catgttcaat ttcgtcggcg agagtaacaa
    21001 actccgaagg ccgctttaag tttttgcgga aaaaagtgga aagatactgc accagctggc
    21061 tggcctgttc gctgtcgcgg cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa
    21121 aatggggatt cacctgggcg tgaagcagtt tgatctctga ctgggtgagc atcgcttttt
    21181 gccgctcata ttgcccggca aggatctgcg ccgaaagcaa ttgcgcaatc ccctcaccca
    21241 acgtgcggtt gattgaactg aataaacggt ttttggcttc atacaatttg atggtgccca
    21301 tcacccgctg attttcacca cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg
    21361 gatgcaaaga gcaacggtaa ggtacttcgt tgccatcagc gtagaccact tcaccggttt
    21421 caatcgcttt taaagtataa gttgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac
    21481 caattccggt aaaggccagc aatttctctc gatcggtaat cgcgactgca ccaatatcca
    21541 gttcctgata cagcacctga gccactttca tgctgttcac ttcgttaaac ccctggcgca
    21601 aaatgccttc cgtcgaggct gccactttca gcgcagtggc agaaaaagcc gaagtgtatt
    21661 tttcaaacat cgcgcgttta tcgagcaata tacgcataaa cagcgccgcg ccgacggtat
    21721 tggtgaccat cattggcgca gcaatattac tcaccagacg caccgcatct tcataaggtc
    21781 gggcgatcgc aaggatgatc agcatttgca ccatttcagc gacgaacgtg acggcaccgg
    21841 cggtaatggg gttaaagact ttatcagtgc gcccgcggcg gatcaggatg ctgtgtacca
    21901 ggccaccgag taatccttca acgatggtcg agatcatgca acttaacgcg gtcatgcccc
    21961 ccatcgaata tcgatgtaag ccgccggtca gcccaaccag cccaccgacg accggaccgc
    22021 cgagtaagcc gcccattacc gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt
    22081 gcaacccaaa ccaggtgccc atgatgcaga agatggaaaa gacgatgtag cagagaaatt
    22141 tatgcggcag acgaaccgtg acctgcatta acggtatgaa taatggcgtt ttactcatta
    22201 accatgcaat gactaaaaaa acgcacatct gctgaagcag cagcaacacc agattaaaat
    22261 cgtacatacc cgcaaaccac acttcccttt aaaacgcgta acatacattg cctgcgttta
    22321 actttctttg aactcttgca gaaaaatgag aattcgtgag tacgatcact caaaatcgcc
    22381 tggcaaaaat aaaatcaccc tatagatgca caaaaaacgg gcaaaactac ctggttcgca
    22441 aaactgcgtc taaagttaaa ccgggacctc gcgagcaagg gtgagacgat ggcgctttac
    22501 acaattggtg aagtggcgtt gctttgtgat attaatcctg tcacgttacg cgcgtgacgc
    22561 aacattttta aaaatcgacg aagatcgtcc aggagcgcca tttttcaagg gttggacaga
    22621 tgattcgttt tcgatgtcga cgaacttcga cggatttcgc cgataaaaag tgtcccatac
    22681 atgccccata ggcaatatta ccaattgaca ctggttatcc acacagctaa aaataacacc
    22741 tgtacataac tacagttaca ggagggggtt atgcgtgttg agatctgtat cgctaaagaa
    22801 aaattcagca aaatgccaaa aggctctgta cctgctctac aacaggaaat gtctcgccgt
    22861 atcagaaaac
//
LOCUS       DBBGLQ010000065        22818 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.065, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000065 DBBGLQ010000000
VERSION     DBBGLQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.065"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(59..967)
                     /gene="melR"
                     /locus_tag="ACIYSB_003480"
     CDS             complement(59..967)
                     /gene="melR"
                     /locus_tag="ACIYSB_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGC5430457.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1250..2605
                     /gene="melA"
                     /locus_tag="ACIYSB_003481"
     CDS             1250..2605
                     /gene="melA"
                     /locus_tag="ACIYSB_003481"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGC5430458.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKKASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2708..4129
                     /gene="melB"
                     /locus_tag="ACIYSB_003482"
     CDS             2708..4129
                     /gene="melB"
                     /locus_tag="ACIYSB_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGC5430459.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADIVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(4268..4897)
                     /gene="yjdF"
                     /locus_tag="ACIYSB_003483"
     CDS             complement(4268..4897)
                     /gene="yjdF"
                     /locus_tag="ACIYSB_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGC5430460.1"
                     /translation="MTRTLKPLILNTSALTLTLILIYTGISAHDKLTWLMEVTPVIIV
                     VQLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5020..6666)
                     /gene="fumB"
                     /locus_tag="ACIYSB_003484"
     CDS             complement(5020..6666)
                     /gene="fumB"
                     /locus_tag="ACIYSB_003484"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGC5430461.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEETLSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6744..8084)
                     /gene="dcuB"
                     /locus_tag="ACIYSB_003485"
     CDS             complement(6744..8084)
                     /gene="dcuB"
                     /locus_tag="ACIYSB_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGC5430462.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8655..9374)
                     /gene="dcuR"
                     /locus_tag="ACIYSB_003486"
     CDS             complement(8655..9374)
                     /gene="dcuR"
                     /locus_tag="ACIYSB_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGC5430463.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9371..11002)
                     /gene="dcuS"
                     /locus_tag="ACIYSB_003487"
     CDS             complement(9371..11002)
                     /gene="dcuS"
                     /locus_tag="ACIYSB_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGC5430464.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11183..11413
                     /gene="yjdI"
                     /locus_tag="ACIYSB_003488"
     CDS             11183..11413
                     /gene="yjdI"
                     /locus_tag="ACIYSB_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGC5430465.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            11425..11697
                     /gene="yjdJ"
                     /locus_tag="ACIYSB_003489"
     CDS             11425..11697
                     /gene="yjdJ"
                     /locus_tag="ACIYSB_003489"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGC5430466.1"
                     /translation="MEIREGHNKFYINDKQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11924..12220
                     /gene="ghoS"
                     /locus_tag="ACIYSB_003490"
     CDS             11924..12220
                     /gene="ghoS"
                     /locus_tag="ACIYSB_003490"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGC5430467.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12248..12421
                     /gene="ghoT"
                     /locus_tag="ACIYSB_003491"
     CDS             12248..12421
                     /gene="ghoT"
                     /locus_tag="ACIYSB_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGC5430468.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12540..14057)
                     /gene="lysS"
                     /locus_tag="ACIYSB_003492"
     CDS             complement(12540..14057)
                     /gene="lysS"
                     /locus_tag="ACIYSB_003492"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGC5430469.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(14294..15751)
                     /gene="dtpC"
                     /locus_tag="ACIYSB_003493"
     CDS             complement(14294..15751)
                     /gene="dtpC"
                     /locus_tag="ACIYSB_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGC5430470.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(15810..17957)
                     /gene="cadA"
                     /locus_tag="ACIYSB_003494"
     CDS             complement(15810..17957)
                     /gene="cadA"
                     /locus_tag="ACIYSB_003494"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGC5430471.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(18037..19371)
                     /gene="cadB"
                     /locus_tag="ACIYSB_003495"
     CDS             complement(18037..19371)
                     /gene="cadB"
                     /locus_tag="ACIYSB_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGC5430472.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(19736..21274)
                     /gene="cadC"
                     /locus_tag="ACIYSB_003496"
     CDS             complement(19736..21274)
                     /gene="cadC"
                     /locus_tag="ACIYSB_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGC5430473.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQKILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(22111..22671)
                     /locus_tag="ACIYSB_003497"
                     /pseudo
     CDS             complement(22111..22671)
                     /locus_tag="ACIYSB_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         5910 a         5344 c         5558 g         6006 t
ORIGIN      
        1 ttttcactgc ttattatcga cggctaaact atttttttgg ctgatactga tatcgtcttt
       61 agccgggaaa cgtctggcgg cgctgttggc taagtttgcg gtattgttgc ggcgacatgc
      121 cgacatattt gccgaacgtg ctgtaaaaac gactacttga acgaaagcct gccgtcaggg
      181 caatatcgag aatactttta tcggtatcgc tcagtaacgc gcgaacgtgg ttgatgcgca
      241 tcgcggtaat gtactgtttc atcgtcaatt gcatgacccg ctggaatatc cccattgcat
      301 agttggcgtt aagtttgacg tgctcagcca catcgttgat ggtcagcgcc tgatcatagt
      361 tttcggcaat aaagcccagc atctggctaa cataaaattg cgcatggcgc gagacgctgt
      421 ttttgtgtgt gcgcgaggtt ttattgacca gaatcggttc ccagccagag aggctaaatc
      481 gcttgagcat caggccaatt tcatcaatgg cgagctggcg aatttgctcg ttcggactgt
      541 ttaattcctg ctgccagcgg cgcacttcaa acgggctaag ttgctgtgtg gccagtgatt
      601 tgatcaccat gccgtgagtg acgtggttaa tcaggtcttt atccagcggc caggagagaa
      661 acagatgcat cggcagatta aaaatcgcca tgctctgaca ggttccggta tctgttagtt
      721 ggtgcggtgt acaggcccag aacagcgtga tatgaccctg attgatattc actttttcat
      781 tgttgatcag gtattccaca tcgccatcga aaggcacatt cacttcgacc tgaccatgcc
      841 agtggctggt gggcatgata tgcggtgcgc gaaactcaat ctccatccgc tggtattccg
      901 aatacagcga cagcgggctg cgggtctgtt tttcgtcgct gctgcacata aacgtatctg
      961 tattcatgga tggctctctt tcctggaata tcagaattat ggcaggagtg agggaggatg
     1021 actgcgagtg ggagcacggt tttcaccctc ttcccagagg ggcgagggga ctctccgagt
     1081 atcatgaggc cgaaaactct gcttttcagg taatttattc ccataaactc agatttactg
     1141 ctgcttcacg caggatctga gtttatggga atgctcaacc tggaagccgg aggttttctg
     1201 cagattcgcc tgccatgatg aagttattca agcaagccag gagatctgca tgatgtctgc
     1261 acccaaaatt acatttatcg gcgctggttc gacgattttc gttaaaaata ttcttggtga
     1321 tgtgttccat cgcgaggcgc tgaaaacggc gcatattgcc ctgatggaca ttgaccccac
     1381 ccgcctggaa gagtcgcata ttgtggtgcg taagctgatg gattcagcag gggccagcgg
     1441 caaaatcacc tgccacaccc aacagaaaga agccttagag gatgccgatt ttgtcgtggt
     1501 ggcatttcag attggcggtt atgaaccttg cacggtgact gatttcgagg tctgtaagcg
     1561 gcatggtctg gaacaaacca ttgccgatac gttggggccg ggcggtatta tgcgcgcgct
     1621 acgtaccatt ccgcatctgt ggcaaatttg cgaggacatg acggaagtct gccccgatgc
     1681 caccatgctc aactatgtta acccaatggc gatgaatacc tgggcgatgt atgcccgcta
     1741 tccgcatatc aaacaggtcg ggctgtgcca ttcggtgcag ggaacggcgg aagagttggc
     1801 gcgtgacctc aatatcgacc cagctacgct gcgttaccgt tgcgcaggta tcaaccatat
     1861 ggcgttttac ctggagctgg agcgcaaaac cgccgacggc agttatgtga atctctaccc
     1921 ggaactgctg gcggcttatg aagcagggca ggcaccgaag ccgaatattc atggcaatac
     1981 tcgctgccag aatattgtgc gctacgaaat gttcaaaaag ctgggctatt tcgtcacgga
     2041 atcgtcagaa cattttgctg agtacacacc gtggtttatt aagccaggtc gtgaggattt
     2101 gattgagcgt tataaagtac cgctggatga gtacccgaaa cgctgcgtcg agcagctggc
     2161 gaactggcat aaagagctgg aggagtataa aaaagcctcc cggattgata ttaaaccgtc
     2221 acgggaatat gccagcacaa tcatgaacgc tatctggact ggcgagccga gtgtgattta
     2281 cggcaacgtc cgtaacgatg gtttgattga taacctgcca caaggatgtt gcgtggaagt
     2341 agcctgtctg gttgatgcta atggcattca gccgaccaaa gtcggtacgc taccttcgca
     2401 tctggccgcc ctgatgcaaa ccaacatcaa cgtacagacg ctgctgaccg aagctattct
     2461 tacggaaaat cgcgaccgtg tttaccacgc cgcgatgatg gacccgcata ctgccgccgt
     2521 gctgggcatt gacgaaatat atgctcttgt tgacgacctg attgccgccc acggcgactg
     2581 gctgccaggc tggttgcacc gttaaaacgc gactaaacgc tactgcgccg ggggatttat
     2641 tccggcgcac acctctgacg ataccaataa cagaaggcgg gcgttggtaa cagcgacccg
     2701 ataccctatg agcatttcaa tgactacaaa actcagttat ggatttggag cgttcgggaa
     2761 ggattttgcg atcggcattg tgtatatgta cctcatgtat tactacaccg atgtcgtcgg
     2821 gctgtctgtg ggtttggtcg gtactttgtt tctggtggcg aggatctggg atgctattaa
     2881 cgatccgatt atgggatgga ttgtaaatgc tacgcgatcg cgatggggta agttcaaacc
     2941 ctggatcctg atcggtacgt tggcaaactc tgtaatctta tttctcctct ttagtgcgca
     3001 tctgtttgaa ggtactactc agattgtctt tgtttgcgtg acctacatcc tctggggcat
     3061 gacttacacc attatggata ttcccttctg gtcgctggtt ccaaccatca cgctcgataa
     3121 acgtgagcgc gaacaactgg ttccttatcc gcgttttttt gccagtctgg caggctttgt
     3181 tacggcaggt gtgacgctac catttgttaa ttatgtcggc ggtggcgatc ggggatttgg
     3241 ctttcagatg ttcactctgg tactgatcgc cttttttatt gtttcaacca tcatcactct
     3301 gcgcaatgtg catgaagtct tttcgtcaga caatcaaccg tctgctgaag gaagccatct
     3361 gacacttaaa gccatcgttg cgctaattta taaaaacgat cagctttcat gcctcttggg
     3421 tatggctctt gcttataatg tagccagcaa cattattacc ggctttgcta tctattattt
     3481 ctcatatgtt atcggtgatg cggatttgtt cccctattat ctgtcgtatg cgggagctgc
     3541 taacctggtg acgttagtat tcttcccacg cttagttaaa tcattatccc gacgcatttt
     3601 atgggccgga gcatctattc ttccggtgtt aagctgtggt gttctcctgt taatggcatt
     3661 aatgagctat cacaacgtcg tcctcattgt gattgcgggt attttgctga atgtgggaac
     3721 ggcgcttttc tgggtattac aggtcatcat ggtggcagat atcgttgatt acggtgaata
     3781 taaactgcac gtacgctgtg aaagtatcgc ttactccgtg cagactatgg tggtgaaggg
     3841 cggttcagcc tttgcggctt ttttcattgc ggttgtgtta gggatgattg gctatgtacc
     3901 gaatgttgaa cagtctacgc aagccctatt aggtatgcag tttattatga ttgctctacc
     3961 aactctgttt ttcatggtaa cgctgattct ctacttccgt ttctatcgcc tcaatggtga
     4021 cacgctgcgc aggatccaga tccatctgct ggataaatat cgcaaagtac cgcccgagcc
     4081 tgttcatgct gatattccgg tcggtgcagt gagtgatgtg aaagcctgac gtggacaata
     4141 gctaacgaaa atggcctgat gcgatatgtt tatcaggcca cgtcttaatg taatgctttg
     4201 gatttgcaaa ttttgtagac cggataagga attcacgccg catccggcat caacaaagcg
     4261 caagttgtta tccggttatc aagccaaagc gccgtagctg gcggcaatga aagcgagcga
     4321 ggaatatcac cgtcgttaat gcgccaagca gcgcgcagaa catatcggat tgcgtgtccc
     4381 actggtcgcc ctgggtgccg agaaaatcat ccgctccctg acccattgcc agcgctgccc
     4441 accactcgat taattcatac atcgcgctta tcgccagcgc cacgcagcag accaggaacg
     4501 ccaccatttt acgtccgcgc acgtacatcc cgcgaacgag aatttctcgt gccaccagtg
     4561 caggcaccag cccctggaaa aagtgcccca gcttgtcata cggattacga ctcaacccca
     4621 gccattcctg cacctcaaaa ccaacgggga ctttcgcgta ggtgtattgt ccgccgacca
     4681 tcaggatgat ggcgtgaagg aaaatgagcg tatagagcag cggcgttaac ggataacgtc
     4741 tggcggtggc aagcagtagc tgcacgacaa taatcaccgg tgtcacttcc atcagccagg
     4801 tgagtttgtc atgggccgaa atgccggtat aaatcaggat taacgttagc gtcagtgcgc
     4861 tggtgttaag aattaacggc ttgagtgtgc gggtcatggt gagttcgcaa atcagggaaa
     4921 atactgacta ttcaccagca agcgtgaaat tacaatcgca aaagaggtgg cccaggggga
     4981 tcacctggca gcatgctgcc aggcgctggg ccgaagaggt tacttagtgc agttcgcgca
     5041 ctgtttgttg acgatttgct ggaagaagtc gttacctttg tcatcgacca ggataaacgc
     5101 cgggaaatct tctacttcga ttttccagat agcttccata cccagctccg gataagcgac
     5161 gcactccaga tgcttgatgc tctgctgcgc cagtaccgcc gccggaccgc cgatgctacc
     5221 gaggtagaag ccgccgtgtt tatgacacgc gtcggtaacc tgctgactgc ggttaccttt
     5281 cgccagcatg atcatgctgc cgccgtggga ttgcagcaga tccacgtagg agtccatacg
     5341 gcctgcggtg gttgggccaa gtgaacctga tggataaccg gcaggggttt tcgccggacc
     5401 cgcgtagtag atcgggtgat ctttgatgta ctgcggaagt tctttaccgg cgtcaatcag
     5461 ctctttcagc ttggcgtgtg caatatctcg gcccacgata atggtgccgg tgagcgacaa
     5521 acgagtggat accgggtatt gcgaaagctg ggcgaggatc tctttcatcg ggcggttaag
     5581 gtcaactttc accgcttcgc cttcaccggc ctggcgcagt tcttgtggaa tgtactggcc
     5641 tgggttgtgt tccagttttt cgatccagat accttcgcgg ttgattttcg ctttaatgtt
     5701 acggtcagcg gagcaggaga cgcccatgcc gaccgggcag gatgcgccgt gacgtggcag
     5761 acggataacg cgaatgtcgt gcgcgaagta tttaccgcca aactgcgcgc caagaccgag
     5821 tttctgggcc tcttccagca gttcctgttc cagctggaca tcgcggaacg cctgaccatg
     5881 ttcgttccct tccgtcggca gttcatcgta atagtgagcg cttgctaact tgacggtttt
     5941 caggttagtt tccgcagacg taccgccaat cacaaacgcg atatggtacg gcgggcaggc
     6001 tgcagtaccg agggtacgca ttttctcgac gaggaagttt ttcagtttgc cgggagtcag
     6061 cagggctttg gtttcctggt agagatacgt tttgttggca gagccgccgc ctttcgcaac
     6121 gcaaaggaat ttgtactcat cgccatctac cgcgtacagg tcgatttgcg caggcaggtt
     6181 agtgccggtg ttgacctctt tgtacatgtc cagcgccgca ttctgtgaat agcgcaggtt
     6241 atcttcgata taggtgttat agacgccttt cgacagcgtt tcttcatcac cgccgccggt
     6301 ccacacgcgc tggccttttt taccgacgat gatcgcggtg ccggtatcct ggcaggtcgg
     6361 cagcacgcct ttggcggcga tttcggagtt tcttaagaat tgcagcgcca cgtacttgtc
     6421 gttttcgctg gcttctggat cgtgaagaat agccgcaacc tgtttctggt gtgccgggcg
     6481 gagcataaaa gaagcgtcgt gaaaggcttg ctgcgccagc agggtcaggg cttctggttc
     6541 cactttcagg atggtttcgc cgtcgaagtc ggcaacgcta acgtaatcgg aagtgagtag
     6601 atagtattcg gtattgtctt tccccatcgg gaaaggtgcc tggtagataa agggtttgtt
     6661 tgacatagct tccagcctgt aactctgtat ttgttattcg aaaatggcgt gccgcgtagt
     6721 cacgggcggc acgcaaagtg catttataag aacccgtaca tcgcggcgaa gatccagccg
     6781 aagacgcacg atacgctcac accaatcaac cccggcagaa taaagctgtg gttgatgacg
     6841 aagcgaccga tgtgggtggt gccggaacgg tcaaactgaa tcgctgccag atcgctcgga
     6901 taagtcggca ggatgtaata accgtagcaa gccggtgctg aagccacgat gtatgccgga
     6961 tcaacgccga tcgccagcgc gaccggaaca atcgccgcca gcgccgcagc ctgagagttt
     7021 acaaacttgg aaaccagcag cagaacaatg gcataggccc acggatactc tttcaccatt
     7081 tcacccagta cgccctgaat ttcagacata tgcgcaccga acatggtttc tgccatccat
     7141 gcgataccgt acaccgccac gatggcgatc ataccggaac ggaagacttc gttttttgag
     7201 atagacgcgg gattggtttt ggtcaggata ataatcagcg ccccggtcag cagcataaac
     7261 atctgaataa ccagtaccat cgacagcggt ttgccgccga aggatggacg caggtccgaa
     7321 tcagcaccaa gaagggcgac tacagcgatt gccccgagga aaatccacat tgccagccag
     7381 ttgcttttcg gcagtttttt atccagcagc gtcgcggtat caccgtaaac atactcacgg
     7441 ttttccggta cggagatgaa tttctggaac tcttcgtctt tatccagatc tttaccgcgg
     7501 aaccagctga agataccgat cgccaggata ccgattaacg tcgatggaat ggtgattgcc
     7561 agcagatcga ggaactcaag atggcgacca tcaaaggtga cattacccag catcgcaacc
     7621 agagacacga ccgcaaccga caccggactg gcgataatcc ccatctgtgc accgatagaa
     7681 cttgccgcca tcggacgttc cggacggatg ttgttcttaa tggcgacgtc gtagatgatc
     7741 ggcagaatgg tgtaaaccac atgacccgta ccgcaaagaa tggtcagtgt acaggtcaca
     7801 aacggcgcga caattgagac atatttcggg ttgcggcgca gcagcttctc ggcaatttgc
     7861 agcatgacat caagaccgcc cgaagcttgc aaggtcgccg atgccgccac caccgcaatg
     7921 ataaccagca tgacatcaac tggtggttta cctggctgaa ggtggaagac gaagaccaga
     7981 atgaccagac cgataccgcc taataaaccc agcgcgatac caccctttct ggcaccataa
     8041 aacagacata tcagtattat gataagttgg atagtaaata acatgtgtga accctcgcga
     8101 taatcctatt taaatttttg ctgaatagat cacagtcacg ttctgttttg tatgaactgt
     8161 tttcagagcc tgactaaata tccgtttggt cgttaccggc tttagcaaat acctcacagt
     8221 gaatattggc tgattaaagc agcggcaaat ttatgcactg tgataagcgg ctttttcaga
     8281 gagaaagact ctctggcgat gggtttatag tttgtactgc gcagatgtta atgttttgtt
     8341 tgctgttatc ttattgatat ttatcggtct attttttgtt ctcagggcag atgtcattag
     8401 gtttatagat taatctgatc tacccatttg tgggtaaaaa tacacataat gcgggtgaca
     8461 taatagttaa ttaacttttg ttagcgtttt gaaattaaaa acaccgttca cctgaagaga
     8521 tattaatttt tagcgatgat ggagggataa ttatatttga tctggcacaa gttttactga
     8581 tgaaggatgt aacttgtgcc aggggtattt tgcattacgg taataattat tacttacaga
     8641 taaccagcga tccgttattg gcaatattgt ttcagtagtg agtagtgttc tgcctgaata
     8701 cggtaacggt aaactggacg ccccgtgacg ccataatgga tactggtgaa caagatgtgg
     8761 cagttgacca gccagatgag gtatttacgg caggaaacac gcgaaatgtt aacctcgttg
     8821 gctagctcgt cggttgaaaa ttcatagtcc tgatgcgcgt caatccactg gcacagtgtg
     8881 cgtaacgtct gcggcgttaa gccttttggc aagcgacgag gatcctgttc gttggagctg
     8941 ctgccgtgga ttagctgatc aagctcggcc tggtcataat actgatgttt ttccagcgcc
     9001 attttctttt gccgccagcc ggtgagcgcc tcttcaaagc gggaagcctg gaagggtttg
     9061 atcaggtaat ccacgacacc gtaatgcagc gaatctttaa tggttgccgc atcggctgcg
     9121 gaggagatga caatcacatc acttttgcaa cgcgcgttat gcaggacagg cagtaaatcg
     9181 agcccgttct ctttttgcat atagatatcg agcaatatca ggtcgatagg cgtatcgcta
     9241 ttgaagataa tctctttggc tttctccagc gtcgaggctg ttccacagca ttgaaagcct
     9301 gggatttgtg ctacgtatcg gcgattcagc tccgcgacca ttgcgtcgtc atcgataatt
     9361 aatacattga tcatctgttc gacctctccc cgtcccaggg tatctggaca aaaaattgtg
     9421 tgaaaatccc gggttccgat tccacggcga tgctgccgcc gagattttct acctgttgtt
     9481 tgacaagtgc taaaccgacg cctcgctcgc ttccttttgt cgagacacct ttgtcaaaaa
     9541 tgtgatcgat tttatcgggt gcgatccccg gtccatcatc attaacttca cagtgcagcc
     9601 agccgtgacg gtagtgcaat gttacgctaa tttcgcctcc gggttccggc cctaatgcct
     9661 ccagcgcgtt ttctatcaga tttcccaacg tggtaatcag cgtcgcgacc tggtcctcac
     9721 tgccgctgtc tggcagctgg ctttcactgt ttaaaatcag cgtatggcct aaatcggtcg
     9781 cgcggttaat cttgctgatt aaaaaaccag cgataaccgg agatttgatc ttacccagca
     9841 gagagccaat ctcttcctga tagttattgg ctgttttgag aatgtaatct tccaactgct
     9901 tataactctt cagatgcaat aatccgagaa tcacatgcaa tttattcata aattcgtggg
     9961 atcgttcacg aagtgcgtca gcatagttga ccagaccgtc gagtcgctgc atcagtttac
    10021 gtacttcagt tttgtccctg aaggttgaaa tggcaccgat gataacgcca ttactgcgca
    10081 ccggaacggt gttgatcagt aatagccggt ctttaatcgt aatctcttcg tcgcggcgcg
    10141 gggtaccgtc gcgtaacact tccgagacat ctaccacctg tgaccatgag tggcttagcg
    10201 tcgacagttt ctcatcgtcc tgcgacttac ggtaattcag caattcttgt gcggcatcgt
    10261 tgatcagcgt gacctcgccg cgatcgtcca cggcaacgac gccttctttg atagactgca
    10321 acatggcctg gcgttgctca aacagcgtgg agatttcgta gggttccagg ccgaaaagga
    10381 tttttttcag taccttaacc agaatgcagg tgccaatcag tccgaccagc atgccaaata
    10441 ataccgacca gataatgctc cagcgactgt cattgatctg ttgggtcaca cggcttaact
    10501 caaggccgat cgccaccacg ccaatttgtt tatgattttc atcgtagatg ggggtaaata
    10561 cgcgtaaagc ctgcgccaga aaaccgcgat tgatagcgac attttcttcg ccattcagcg
    10621 ctttaaggat gtcatcacct ttaaatggct gaccaatacg ctgggcttca ggatgcgagt
    10681 agcgaagact ttgcatatcg gtaacgacaa taaacagcag atcgttgcgt ttgcgtacgg
    10741 cttccgcgat ggcctggatg ccactctcct gcggtttttt ctgcaagccc tgacggattt
    10801 ccggcgagtc ggcgagggta cgcgccactg ccagtgcctt gttggctagc ccatctcgcg
    10861 tcatatcact gatttgcgag aagtaaatca gatgcaccac caatagcacc gagaacagta
    10921 ccgcactgac cattaagatc actgtggtac tcaatttcat cggacgtttg cgtaacatgc
    10981 ggtagggcaa tgaatgtctc atcagcttcc ttgtgtgaca aatttcttaa gcattatctc
    11041 tgatgaggcg ggtaattcaa agggagtaag aatgattggc tatatagggg aagagactct
    11101 ggcaacggaa actgccagtg ctgtatgaag attccggggc tatgcttata gcgataatca
    11161 tactgatgag agagggaagg tcatggatca ggcgctactg gacgggggtt atcgctgtta
    11221 taccggcgaa aagatcgatg tctatttcaa cactgcgata tgtcagcatt ctggcaattg
    11281 cgtacgtggc aacggcaagt tatttaatct caaacgaaag ccgtggatca tgccggatga
    11341 agtcgacgtc gccactgtgg ttaaagtgat tgatacgtgc ccgagcggcg cgctgaaata
    11401 ccgtcataaa taagcgaggg taaaatggaa atacgcgaag gccacaataa attttacatt
    11461 aatgacaaac aaggcaagca aatcgctgaa attgtctttg tgccgaccgg agagaattta
    11521 gcgattatcg aacataccga tgtcgatgaa agcctgaaag ggcaagggat tggtaaacag
    11581 ctggttgcga aagtcgtgga aaaaatgcgt cgggaaaaac gaaaaattat cccattatgc
    11641 ccatttgcga aacatgaatt tgataaaacg cgggagtatg atgatattcg cagttgatgg
    11701 gagagtacag agtcacgata tttttcattc tctccgcgat gtgatgcagg agagcatctg
    11761 aagggtaggg ggatgcacaa agaatgggca gagagcgcgt ttttttgtcc caagtcatcc
    11821 cctttactga gcaaaaaaaa gaatatctcc tatatgagaa tcatcaatcg gggttaataa
    11881 gttttgcgtc cccagagcgt ttaatattga taggagtcat attatggaag gtaaaaacaa
    11941 gttcaatact tatgttgttt cttttgatta tccatcatct tattcctcag tgttcttaag
    12001 attaagatca ttgatgtatg atatgaattt ctcctctatc gtggctgatg aatatgggat
    12061 accacgacaa ttgaatgaaa actccttcgc aataacgaca tcgttagccg caagtgaaat
    12121 cgaagattta atcaggctca aatgcttaga cttaccggat attgattttg acctcaacat
    12181 tatgacagtt gatgactatt tccgtcagtt ttacaagtag ccaggaagga taagaagaac
    12241 ataaaaaatg gcactattct ctaaaatatt aattttttat gtgattggtg tgaacatatc
    12301 ctttgtcatt atctggttta tctcacatga gaaaacacat attcgtttac ttagtgcatt
    12361 cctggtcgga ataacctggc caatgagtct gcctgtggca ttactttttt ctctctttta
    12421 ggagctgtag ttgagtggac ccgctggtta tcgtgaaacg aagcgtctct cctcaaaact
    12481 ggatttgcgg aacgatgaac gaagcagtcg cctgactgct tcgttcatta aagtgaaatt
    12541 tatttctgtg ggcgcatcgc cgggaagaga ataacgtcgc gaatagtatg gctgttagta
    12601 aacagcataa tcattcggtc gataccaata cccagaccag cggttggcgg cagaccatat
    12661 tccagcgcag tcacgtaatc ttcgtcatag aacatggctt cgtcgtcacc tgcagcttta
    12721 gcattaacct gttcctggaa acgttcagcc tgatcttctg cgtcgtttaa ttcgctaaaa
    12781 ccattaccga tttcacgacc accgatgaag aattcaaaac ggtcggtgat ttccgggtta
    12841 acatcattac ggcgtgccag cggggacact tctgccggat attccgtaat aaaggttggc
    12901 tgaatcagat gtgcttctgc cacttcatca aagatctctg tgacaatacg tcccaacccc
    12961 cagcttttct ctaccgtaat accgatagat tcagctaatg ctttagcagc atcaaaatta
    13021 tccaggtcgg ccatatcggt ttctggacga tattttttga ttgcttcgcg catggtgagt
    13081 ttttcaaacg gtttgccgaa atcaaacaca tgctcgccat aagtgacttt agtggtaccc
    13141 agaacctctt gtgccagggt gcggaacagt gactctgtca gttcaatcaa atcgtggtaa
    13201 tccgcatacg ccatgtagag ttccatcatt gtgaactcag gattatggcg aacagaaata
    13261 ccttcattac ggaagttacg gttgatttcg aatacccgtt caaaaccgcc tacaaccaga
    13321 cgtttcagat acagctccgg cgcgatacgc agatacatat ctaaatccag agcattatga
    13381 tgggtaataa acgggcgagc agatgcccca cctggaatta cctgcatcat cggggtttct
    13441 acttccataa agccgcgcgc gaccatgaat tgacggatag cggccagaat ttttgaacgg
    13501 acaacaaacg tttgacggga tttatcgtta gcgatgaggt ccagataacg ttgacgataa
    13561 cggacttcct gatcctgcag accatggaat ttatctggta aaggacgtag tgctttagtc
    13621 agcaggcgca gctcagtaca gtgaatggaa agctcacccg tttgcgtctt aaacagcgta
    13681 ccgcgggcac cgataatgtc acccagatcc cattttttaa actgatcgtt ataaacacct
    13741 tctggcaggc tatctcttgc aacgtacagt tgaatacggc caccgacatc ctgcaacgtt
    13801 acaaaggagg ctttccccat gatacgacgg gtcatcattc ggccagcaac cgagacttca
    13861 atgtttaagg attccagttc ctggttatcc ttcgcatcaa actcttcgtg caactggtca
    13921 gaggtatggt cgcggcgaaa atcattggga aacgccacac cttgctgacg tagtgccgcc
    13981 agtttttcgc ggcgatttct cagttcatcg ttaaaatcaa tagcctcatt ggctccccgt
    14041 gtttcttgtt cagacatttt ggttcctcta aatccagctt tcaatttagc gtagataaag
    14101 agacagatcg gtacactgta ttatctgcct cgactattag taactcagtt tatcttatcg
    14161 aaactaacgc ttacctcaag ttgatatcac taataaataa ccatcaaaat ggtttaaatg
    14221 acttattaat atcgatttgt tagtgttata aactgaaagt taatttattc ataaccaatt
    14281 gtttttacga cagttaatcg ttgctctcct gtatcatatt cgtcggcgtg ctaaacagaa
    14341 aacgggtagc aaaggccaat acgacgatga tcgcgacaca agccaacgtc cactctccca
    14401 tctgagaaaa gaatcgctgg taagctgcaa ttgccatacc gctaatttgc gactctgtcg
    14461 tctgctgtgc cacgacgcct gccagccagt tggcgaccgc gcctgttgcc agcatataaa
    14521 tcccggttaa tacgccagac attttcagac gcgtgatttg cgcaatcgcc accgggtcaa
    14581 taaagagttc ggcaaagccc attagcgcca gcccggatat catcacgccc attgacgctt
    14641 gaccgtcagc cgctgcatgt cgggcatcaa atgccaacaa cataaagcca caagccatca
    14701 gcagtaagcc aaaggcaaac ttcagccaga cgcgcaatgt tgagttgccg cggctttctg
    14761 gcgacgccag ccaggccagt acaaccccag cgagcatcac cgcaattgca ttcaccgact
    14821 ggaatagtgc tgtaggtact tcaatattga atgcctgacg attcacaaag cgatcgataa
    14881 acaagctgat ggtactgccg ccctgttgtg ccagtaccca gaacaatgtc ccgacaaaca
    14941 tcaacaatac aatttgccaa agagcacggc gatgttcggg gaatttgatc atcatgcggg
    15001 caatgatttg tgcggcaatg aggcaaacga tcgccagcaa atatccggac cagtcgttct
    15061 ccagcagcag agtaaaaaat actggggcta aacagagcat caccactaac cagctccata
    15121 ctggtaaggc aaatttgaca ctagtgagcg cttttttatc catactacgt gtggactgga
    15181 aatgacgatg accgcttaag aaaatcaaca aaccgataaa catgccgcca cccgcaaggg
    15241 caaagccaac atgccatcca taccactgag cagccaggcc gcaggcgatt ggggctgcga
    15301 tagaaccgat attgcccgca gcatacagca gcgaaaaacc gccatcacgt cgatgatcgt
    15361 tctcgtcgta gagctcgcca agcaaacagc tgatgtttga tttgaataaa ccgtagccac
    15421 aaataatgat tgccagcgcc agatacaggc taaaggttga atttgtatca atacccagca
    15481 ccacatggcc aagggtcatt aacagcgcgc cggcaatcac tgcagtgcgg ttgccgagca
    15541 ggcggtcggc aagccagccg ccgagaatag gggtaacgta aaccagagaa gcatatgcgc
    15601 tgaacaggct gatggcatgg ttatcatcaa aaccaagctg atgggtgaga tagagaatga
    15661 gtaaggcacg catgccgtaa aaactgaagt actcccagat ttggatcgcc acgatatagt
    15721 atatcgcgcg cggctgtgag ggtgttttca tgtgttctcc ttatgagcaa aaaagggaag
    15781 tggcaagcca cttcccttgt acgagctaat tattttttgc tttcttcttt caatacctta
    15841 acggtatagc ggccatcagc ctgacggtat gcaccgtgaa tatcggtttc aaagcccgga
    15901 tagtgagcgc cgatttcaca cagcatctgc aggaactcca gaaccggacg gctttcttcg
    15961 gtgatcattt cacccggcat taccagagga actcccggcg ggtacggaag gatcatattg
    16021 gcgttaatac gacctaccat ttcgtcgagg taaacttctt cggtcatacc gtgcagctct
    16081 ttctggaatg cagcatacgg agtcattacc atcgtcggca gcacttcaaa tgcgcgatac
    16141 atcagatccg gcagattgtg gtgaacaatc agtttgtgga tattctgagc cagttcctga
    16201 atacgcatgt tttcatagaa ttcaggatct tcacgataca gagacggcag catgtttttc
    16261 acacgcaggt tcaggtcgaa cgcacgttta aagtcagtca gagcacgcag caggctcagt
    16321 gctttggtct tatcgatacc gatgctgaac aggaacagca ggttatacgg accggttttc
    16381 tcaacaacga tgccatgttc gtcgaggtat ttcgccacga tgctggccgg aataccaaag
    16441 tcgctcatgg tgccgtcttt ttccatcccc ggagtcagca gggtgacttt gatcgggtca
    16501 agatacatgt gctcgttatc gatgtttttg aagccgtgcc aggtgctgtc agaacgcagc
    16561 ggccagcatt cagtcgtatc gatatgatcc ggctgccata catcaaagaa ccagccatca
    16621 gattccgttc tcagacgttt gatctcttta cggaatttga tcgcacgttc aatagaaccg
    16681 ttgatcagac gcttacctgc attgcctttc atcatcgccg cagcggtttc agtggacgcc
    16741 acgataccgt agtgcggaga agtggtggtg tgcatcatgt aggcttcgtt aaaggtttct
    16801 tcgtttacgt cacctttaac gtggatcatg gaagcctgag agaacgccgc cagcagtttg
    16861 tgagtggact gggtttcgta aatcactttc ccttctacac ggccaccgct cataccgcat
    16921 ttaccttcgt aaatcggtga gaagttggtg taaggcaccc acgcggagtc aaagtggatg
    16981 gatttcacat ccagtgtttt cttgatgaag tcggtgttgt acagcagacc atcataggta
    17041 gagttggtaa ttacagcatg taccggccag gttgcgtttg gtgtttcttt cacgcgctta
    17101 gcaatggtag cgtgctggaa ttcactctgt gggataccac caagaatacc gtaagcgtta
    17161 cgggtcgggc ggaaatagat tggcgtaaca tcgctcatca tcatcaggtg ggtcagcgat
    17221 ttgtggcagt tacggtcaat cagaatggtg ctgcctgctg gagcagagta cataccaaca
    17281 attttgttcg cagtggaagt accgttggtc accatgtagc tgcggtctgc gttaaagacg
    17341 cgagcgatat actgttctgc ttctttgtgt ggaccactgt gatccagcag agaacccagt
    17401 tcagatactg aaatggaaat atcagatttc atggtattcg gaccaaagaa atcatagaac
    17461 aggctaccta ccgggctttt ctggaatgca gtaccgccca tgtgaccagg agtacagaaa
    17521 gtatatttac cttcacgaac atatttaaac agtgctttag tcagcggagg cagaatagtg
    17581 ttgatatatt cgtcagtggt ctgcttgatc ttattagcaa tatcttcagc agcacccagc
    17641 gcatattcaa agaagctaat ctgtaaacgc aggtcattca ggcttacatc gagagtggaa
    17701 tacgtattag cgaacgcgta caacggcagg ttctcgttca ttttgctaat ttcttcgcac
    17761 agctcgagat tatatttatc ccagtcaaaa ataacgccgc acagacgcgc attgttttcg
    17821 atcagtttta ataagtcgtc acggtcgttc gggtaaacaa tctggaagtt cagacgttca
    17881 agcgcgcgat gaagttcacg gatgggttct tctttaaaat aaacccccat gtgattcaat
    17941 attgcaataa cgttcatagt catatctcca ggtaaaaaag gcccctccca acacatggga
    18001 caaaatgaaa ggaggagcct cggaaaatac ttttaattaa tgtgcgttag acgcggtgtg
    18061 gttatccatt gagtggctct ggcgctcgtg cattttgcga gcgtagaaca tcaggataat
    18121 caggctgacg atgaaggtac ctgccagctc gaaggagctt gcgcccatca gcgcgatgaa
    18181 gcagaacacg caacccagta cagagcagat caggctgaca aagttgcgga tgttaacgcc
    18241 ttcaaaacga atcaggtcaa cgcaagagta gaaatacggc agcatagtca gcagtactgc
    18301 gataccggtc agttcaccga acaggtcaga tgctttacca ccggcagagt tcatcagagt
    18361 gataaggatc atcagggcag tcattttcac tgcagccagc agcagacctt ttttcggaat
    18421 accgttgctg tcgacttcac cataaacttt cgggaagtta ccgtcgttag cggcacgtac
    18481 acctgcctgg cctaccaaca tcatccagga gcccagagaa gtcaggcacg caaaggcggt
    18541 gaatgcagaa accagcggcg cagcccagtt accgaggata gttgaagcac tgattgcaaa
    18601 cggagcaccg gaagccgcca ttacagaaga cggatacata ccggaaagca cctgagtcgc
    18661 agcgatgtaa acaatacctg ctaaaccagt acccagcatg gttgccagcg gaacggtacg
    18721 tttcgggttt ttaaccatac cagtacttac agctgcggat tcaacaccca cgaaggccca
    18781 caggcagagc agaatacttt taatgatcgc atgaccatca gtggtatccg cagtattcca
    18841 gttagctgca taagttgccg catcaaacca atgccagcca acaatagcag tcatcaccac
    18901 aggaataaga accagcacca gaccaatagt ggttaaacgg cttacccaag taccgccgag
    18961 catatttaca aaggtaaata cccagacgat agcaatacag gcgatacccg ccggaacagg
    19021 atcatttaat actgggaaga aggtggaaag ataagataca gcggtaatac caatcgccag
    19081 gttaccaatc cagttagcat ggtaataaag aacacctgtc tgaaaaccaa atgcagggga
    19141 aatttctccg gcataagcaa ttgggccacc ttgttgcggg ttttttgttg ccagtcgggc
    19201 atatacatac gccagcgaca ttgcaccaat aatagagata atccaacccc agatagcaat
    19261 accaccgata cttgctaggt tcgcaggtaa taatgcaata ccgctcccca tcatattacc
    19321 ggcaacaaca ccggtacagg caaatagccc gatcttcttg gcagaactca tgctcttctc
    19381 ctaatttcat ttttgaattt ggagtccggg tcatgatgta taactatttc ctgaccagac
    19441 caaactggcg ataagattac tcacgaaaaa aggattaatc ctaaagatta ggtgaaataa
    19501 cacaaaagtt tctgtaagtg agaacttgag gttttttatt aacacatcag gatcgcaagt
    19561 tgatatcatg aaaagataaa catttaatgt ttacaatgga ttgcgtgaca ttctctggtt
    19621 aaatttatgt aataaaaatt atgcggcaaa taaattgccg caacatatta taccaacagg
    19681 aacatacaaa aactcaacaa caaatatttc cgagcataaa tcaacccgga gttacttatt
    19741 ctgaagcaag aaatttgtcg agataaggta caacataagg aacagaagtc tggaatatac
    19801 cattttcaat ccagtaaagg gtgtttgccc ctgggcgtaa attaaaggcg gtgagatatg
    19861 catcagctgc ttcccggttc atccccttca tttcataaac cttgccaagc aacacataat
    19921 ttagccagga catttcaaga tcaatgccag tatttatcgc ctggtaagac tcatctgttt
    19981 taccttttac cagagcactg accgctttta tttgatatat aatggacagg ttgttcaatt
    20041 ccggcagtgt aacaatgtta tctatttctg tgttcagtgc tgctaattgt ttttcatcta
    20101 aaggatgttg agaatggcgc acgatatcaa ctaatgcttt ttctgctctc gcgtaggtaa
    20161 attctgggga tgattgaaca atctcaccta ataattcact ggcacggttc aatgatttat
    20221 catcgccatg cagtaaataa tcatgtgcct gataaaaatt agttaataac gcaccacgat
    20281 gcggcaaaat tttctggagc gtctcctgca ttcgttgtgg ccacggttgg tttaacgctt
    20341 ttgataaact ctccagtaaa tcattttgaa tcgccagctg attaccgtta gtgatgacat
    20401 aacgtttatc cagcatggtt gaaccatctg cattgtctac caattttatc gacataaagc
    20461 attgttgagc acggtattgg cgctgattaa caaacgcaat agataatgtt ttaccggaac
    20521 tgctcggttc atcaatgttg tagttgattt tgtcatgcac cataaaggtg gagaaggtgt
    20581 taagtgatgt cgccaccaaa tcacccacgc ctatcgcgta agagagctga tacggggaac
    20641 tccagctgtt acaactttta tttaccatat taatgtcaat atcgcgtgga ttgagcaaaa
    20701 tacgcgattt gctcatagga agacgtgtat caagacttga aaacgctacc agtgctacac
    20761 agatacctaa cgacaacagg aaaaaaaacc atacccaaaa ggtagtgaat cgtttgcttt
    20821 taactgggga ttgttcaggt ggcgttgcgg tgttttgaat gttaagactg tgggagggag
    20881 aatctgtggc aggaaccgcc tctggtatag ggggaggcga agatagcatt atttcctctc
    20941 cctcttcttc gctgtaccag ataaccggca ccattaattt atagccgcgc tttggtacag
    21001 tagcgatata gacaggacta tcttcatcat tatcttttaa tgacttacgt agttctgaga
    21061 tactctgcgt cacaacgtga ttggtgacaa tacttctctt ccagacatta tcgataagtt
    21121 catccctgct aagtacttcg ccactgtgtt gagcaaagaa aaccagaaga tcgattaatc
    21181 tcggctcaag ggtaagttga cgcccattgc ggctaatttg gtttatggac ggagtaacaa
    21241 gccattcgcc aacgcgaact acaggttgtt gcataataga aactcattcg aaaagggaat
    21301 gatgcaatga taattgccac aacctatttt taccatctat agatgggttt atttacatat
    21361 tattggtgaa tgcaagacgt tatttttacc agccataaac ttcttgatta catagtatta
    21421 cgaaaggatt ttactgagaa ccagaagtaa tattccttac catcaaaatt catcatcttt
    21481 gccaaagaaa aatgttcaga aaataatcca tggaaaattg tccggagcac ttactatttt
    21541 aatggattgt tagtctttgc atgagcaagc gaactgatac atttctcttt gttctcattc
    21601 agaaaatctc atcagtcgcc gttcagaccg gtgttgtgct ttatggatgc gttacgctcc
    21661 tgatgacgtc atttgacgtt caacagcatc acggggccgc acgacatttc acgtcagtta
    21721 gtgctatagc tcaggaacaa attttcccga attgggatat gcccgcaaat tgctggtgat
    21781 gtggggagaa tctggttgag ttcggtagaa ttgatttgga ggcagaacgc ttaaatcgtg
    21841 gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt tatatgaatt tggtgccgga
    21901 ctcggaatcg aagatttctt ctatcatgtt gatatttgtt tgttttgttt ggtttggtga
    21961 aaaatatacc ctcttttgta ccctcagtcc cttttgctgt gaaaaaggaa tcaaacccgg
    22021 gtgttggaag atgaggtgaa aggggggaag cataaaggga ttcaccttaa cagcgtactg
    22081 ctgttaaggt gggggggaac catctcatta tccccactgg tcccacctca ccaggttgtt
    22141 gatgataatt ttattactaa atttgcaggg cgacctggtt ttgtaatccc ggctcagtcc
    22201 tctgaagacg ttgatctccc cccgctcaaa cagctcatcc ttattctggt gcagttgttt
    22261 aaatgtgctc agaatgttcg cttcgctgat ttccggaaaa ttatcatatt ccagtgacct
    22321 gtaccatgta tcacgtgcct gtgcatccat taaggaaagc atcccggaac gctgcatcag
    22381 gttacgccag atttcgcggt caagattccg ggtaatggct ttcattgctg tttctggttt
    22441 ctccatcagc cagcaaccac agcgaaaatt ctgtttcatc gcccagtccg gggcggcttt
    22501 accgccaata ctttccgtca gtgctgagat ttctgtcagt tgccggataa gcgtgtcgat
    22561 ttgcagcagt gtactctttc gtacagccag gatgtgtctg atacttgtgg aacagataac
    22621 ggctgtatgt ccgttgaccg cgttcgggtg ggggattgtg atatatgaca ttatggggtt
    22681 tcctttctgt ttataaacga aaacgccagc tctgttcagg ctggcgtcag gagaatgaag
    22741 tctggtgagt gagattcacc ggttctggtg cagaagttgc agttggtgca gggtacggat
    22801 atcctccctg gcctgtta
//
LOCUS       DBBGLQ010000066        22696 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.066, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000066 DBBGLQ010000000
VERSION     DBBGLQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.066"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(17..169)
                     /gene="hokE"
                     /locus_tag="ACIYSB_003498"
     CDS             complement(17..169)
                     /gene="hokE"
                     /locus_tag="ACIYSB_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGC5430474.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            138..406
                     /locus_tag="ACIYSB_003499"
                     /pseudo
     CDS             138..406
                     /locus_tag="ACIYSB_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            622..1740
                     /locus_tag="ACIYSB_003500"
     CDS             622..1740
                     /locus_tag="ACIYSB_003500"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGC5430475.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1806..2054
                     /locus_tag="ACIYSB_003501"
     CDS             1806..2054
                     /locus_tag="ACIYSB_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGC5430476.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLALAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2119..2487
                     /locus_tag="ACIYSB_003502"
     CDS             2119..2487
                     /locus_tag="ACIYSB_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGC5430477.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2581..3233
                     /gene="nfsB"
                     /locus_tag="ACIYSB_003503"
                     /pseudo
     CDS             2581..3233
                     /gene="nfsB"
                     /locus_tag="ACIYSB_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
     gene            3341..4588
                     /gene="ybdG"
                     /locus_tag="ACIYSB_003504"
     CDS             3341..4588
                     /gene="ybdG"
                     /locus_tag="ACIYSB_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGC5430478.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4669..6045)
                     /gene="pheP"
                     /locus_tag="ACIYSB_003505"
     CDS             complement(4669..6045)
                     /gene="pheP"
                     /locus_tag="ACIYSB_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGC5430479.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFM
                     AFKTLRRK"
     gene            complement(6147..9290)
                     /gene="cusA"
                     /locus_tag="ACIYSB_003506"
     CDS             complement(6147..9290)
                     /gene="cusA"
                     /locus_tag="ACIYSB_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGC5430480.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRGGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9302..10525)
                     /gene="cusB"
                     /locus_tag="ACIYSB_003507"
     CDS             complement(9302..10525)
                     /gene="cusB"
                     /locus_tag="ACIYSB_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGC5430481.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFNWFAKAEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10541..10873)
                     /gene="cusF"
                     /locus_tag="ACIYSB_003508"
     CDS             complement(10541..10873)
                     /gene="cusF"
                     /locus_tag="ACIYSB_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGC5430482.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10900..12279)
                     /gene="cusC"
                     /locus_tag="ACIYSB_003509"
     CDS             complement(10900..12279)
                     /gene="cusC"
                     /locus_tag="ACIYSB_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGC5430483.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASLDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQ"
     gene            12436..13119
                     /gene="cusR"
                     /locus_tag="ACIYSB_003510"
     CDS             12436..13119
                     /gene="cusR"
                     /locus_tag="ACIYSB_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGC5430484.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13109..14551
                     /gene="cusS"
                     /locus_tag="ACIYSB_003511"
     CDS             13109..14551
                     /gene="cusS"
                     /locus_tag="ACIYSB_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGC5430485.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            14701..16938
                     /gene="nrfB"
                     /locus_tag="ACIYSB_003512"
     CDS             14701..16938
                     /gene="nrfB"
                     /locus_tag="ACIYSB_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGC5430486.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            16925..19897
                     /gene="nfrA"
                     /locus_tag="ACIYSB_003513"
     CDS             16925..19897
                     /gene="nfrA"
                     /locus_tag="ACIYSB_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGC5430487.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEKRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEPAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFIAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            19898..20788
                     /locus_tag="ACIYSB_003514"
     CDS             19898..20788
                     /locus_tag="ACIYSB_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGC5430488.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGVVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            20971..21732
                     /gene="envY"
                     /locus_tag="ACIYSB_003515"
     CDS             20971..21732
                     /gene="envY"
                     /locus_tag="ACIYSB_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGC5430489.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSRPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(21778..21854)
                     /locus_tag="ACIYSB_003516"
     tRNA            complement(21778..21854)
                     /locus_tag="ACIYSB_003516"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21818..21820),aa:Arg,seq:tct)
     gene            22097..>22696
                     /gene="fimZ"
                     /locus_tag="ACIYSB_003517"
     CDS             22097..>22696
                     /gene="fimZ"
                     /locus_tag="ACIYSB_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGC5430490.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL"
BASE COUNT         5528 a         6045 c         5676 g         5447 t
ORIGIN      
        1 actccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat
       61 tacgttcctt caccgtaaac tcacacagcg agtctccgac cagaagcgta aatcccagca
      121 ccgttaaaca cagcactatg actgccgcaa gggcatattt cgtcagcatg tttatattgc
      181 ctccatgcga agaagggact accatccaaa ttgttcgggt ttggagtggc agccccaggg
      241 ttgatagaaa tatctcctgg ggcttcatct ttccagacct cagagtaaac ctgaaaccgg
      301 aaagcttcag gcacccgccg ttatcttacc cttcctcaac aaaatagctg tttatttata
      361 cagtgagtcc tgtttcggac ttctcactcc tcatttaatc aactgaagct gaacccgttt
      421 ttttctgcat gactcaatcg tcaaaattct tcgccaccag tcagcactgc cataacttgt
      481 cacattgcaa aagtagccaa attgcttcag gcaccatcag ttcaccatca ccaaaaaaaa
      541 gaaacattcc agaacttccg catctcgccc agccgctccg acttctatac tgaatagaaa
      601 acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga accttttacc
      661 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
      721 tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt aaagcacgat
      781 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
      841 gggcagtttt cagcgatgca gaaagtcgta ttgcaggcag ccacagacca tcatctggaa
      901 atttgcggcg gtggcacgca cccgtttcag aaatggcagc gtcaggaggt atgcgataac
      961 gaacgctatc aacgcacgct ggaaaacttt ggttatctca ttcagcaggc gaccgttttt
     1021 ggtcagcatg tccatgttgg ctgcgccagt ggcgatgacg ccatttattt gctgcacggc
     1081 ttgtcacgat ttgtgccgca ctttatcgcc ctttccgccg cgtcgccata tatgcaggga
     1141 acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc tgataatggc
     1201 ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tgtttcgctg tctgagttac
     1261 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt
     1321 ggcacggtgg aggttcgggt gatggatacc ccgttaaccc ttagccacgc agtaaatatg
     1381 gcgggattaa ttcaggctac cgcccactgg ttactgacgg aacgcccgtt taaacatcag
     1441 gaaaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tgggcttgaa
     1501 ggcgtcatca ccgatccgca cactggagat cgtcgaccgc taacggaaga taccttgcga
     1561 ttgctggaaa aaatcgcccc ttccgcacat aaaattggtg catcgagcgc gattgaggcc
     1621 ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc taatgcgcga tttcgtcgcc
     1681 gatggcggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc cggtgactaa
     1741 gccgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     1801 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     1861 tcctctcttg cctgctgctg cccgcccccg cactggggct ggcgctggca caaaaactgg
     1921 tgaccatgtt ccatctgatg gatcttagtc agctttacac tttattgttt tgtctgtggt
     1981 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
     2041 cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc ttattctcct
     2101 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctggcc
     2161 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
     2221 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
     2281 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
     2341 tatcacatga ataaaaagca ctggatttca gtgtatcccg gcgaagaaat ctccgaagcg
     2401 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     2461 gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat agcgcatttt tctcgcttac
     2521 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt
     2581 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa
     2641 aaacttaccc cggaacaggc cgagcagatc aaaacgctac tgcaatacag cccatccagc
     2701 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     2761 gccaaatccg ctgccggtaa ttacgtgttc acgagcgtaa aatgcttgat gcctcgcacg
     2821 tcgtggtgtt ctgtgcaaaa accgcgatgg acgatgtctg gctgaagctg gttgttgacc
     2881 aggaagatgc cgatggccgc tttgccacgc cggaagcgaa agccgcgaac gataaaggtc
     2941 gcaagttctt cgctgatatg caccgtaaag atctgcatga tgatgcagag tggatggcaa
     3001 aacaggttta tctcaacgtc ggtaacttcc tgctcggcgt ggcggctctg ggtctggacg
     3061 cggtacccat cgaaggtttt gacgccgcca tcctcgatgc agaatttggt ctgaaagaga
     3121 aaggctacac cagtctggtg gttgttccgg taggtcatca cagcgttgaa gattttaacg
     3181 ctacgctgcc gaaatctcgt ctgccgcaaa acatcacctt aaccgaagtg taattctctc
     3241 ttgccgggca tctgcccggc tatttcctct cagattctcc tgatttgcat aaccctgttt
     3301 cagccgtcat cataggctgc tgttgtataa aggagacgtt atgcaggatt taatatccca
     3361 ggttgaagat ttagcgggta ttgagatcga tcacaccacc tcgatggtga tgattttcgg
     3421 tattattttt ctgaccgccg tcgtggtgca tattattttg cattgggtgg tactgcggac
     3481 cttcgaaaaa cgtgccatcg ccagttcacg gctttggttg caaatcatta cccagaataa
     3541 actcttccac cgtttagctt ttaccctgca ggggattatc gtcaatattc aggcggtatt
     3601 ctggctgcaa aaaggcaccg aagcggcaga tattctgact acctgcgcgc agttgtggat
     3661 catgatgtat gcgctgcttt cagtcttctc gttgctggat gttattttga atctggcgca
     3721 gaaattcccg gcagcatctc agttaccgct gaaagggata tttcagggga ttaaactgat
     3781 cggcgcgatt ctggtcggca ttttgatgat ctcgctgctg attggtcagt cgccagcgat
     3841 tctgatcagc ggtcttggtg caatggctgc cgtgctgatg ttggtattta aagatccgat
     3901 tcttggtctg gtggcaggta ttcagctttc cgcgaacgat atgctgaaac tgggcgactg
     3961 gctggagatg ccgaaatacg gcgcggatgg cgcggtgatc gatattgggt taaccaccgt
     4021 caaagtgcgt aactgggaca ataccattac cactattccc acctggtctc tggtttctga
     4081 ctcctttaaa aactggagcg ggatgtcagc atctggcggg cgacgtatta agcgcagtat
     4141 cagtattgat gtcaccagta ttcgttttct tgatgaagac gaaatgcaac gtctgaataa
     4201 agcgcatttg ttaaagcctt atttaaccag ccgccatcag gaaattaatg agtggaatcg
     4261 ccagcaaggt tctacggagt cggtattaaa tctgcgccga atgaccaata ttggaacctt
     4321 tcgtgcctat ctgaacgaat atctgcgtaa ccatccgcgg attcgtaaag atatgacctt
     4381 aatggtacgc caactggctc caggtgataa cggtttaccg ctcgagatct acgcgtttac
     4441 taacaccgtg gtgtggctgg aatatgaaag cattcaggct gatatattcg atcacatatt
     4501 tgcgattgtc gaagagtttg gtctgcgact tcatcagtcg ccaaccggca atgatattcg
     4561 ctctctggcg ggtgcattta agcagtaatt aaaaaaaccg ctctcatcga atggatgaga
     4621 gcggtttcgg atggttgaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg
     4681 cagcgtttta aatgccataa acaggaatac aatccacacc ggcagcagga tcgctgacaa
     4741 gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg caatgcagag
     4801 atagttgccg aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat
     4861 cgctgcacga aaacgcagat gcgccagaca gatcataatc cagttcaaca gcagcgttgc
     4921 taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac
     4981 caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct
     5041 gacgcgagtc aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct
     5101 gttagagtaa accccgctgt tatacactga cagcgatgct accagaatga cgaagttcag
     5161 cgcagaagct accacgttgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt
     5221 ggatttcact tccacccacg gatagagcgc cagtaaaacc accagtgaac cgatgtaaaa
     5281 cagcaggatg cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg
     5341 cgcttcagcg gcagtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc
     5401 cagcgacaaa atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt
     5461 gtcgatactg gctttctcgc cgccgtgacc agaaaacagc agccacaggc caaagccgat
     5521 cataccgatg attgccagca ctttaatcaa cgcaaaccag aactcggttt cgccatataa
     5581 gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca
     5641 cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat
     5701 tcccaccagc acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc
     5761 ccagtattta taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc
     5821 gccaagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac
     5881 agccggaccc gccatctgaa tcgccggacc aatgccaaga aacagaccag taccaattgc
     5941 gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc
     6001 ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt
     6061 gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc
     6121 acacggttaa acggggtatc ctgcttttat ttccgtaccc gatgtcggtg cagccacatc
     6181 agcttatacg ccgccgggat aataaacagc gacagcaaag gtgcggtgat catgccgcca
     6241 atcatcggtg cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc
     6301 ggcagcagac cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcagtacc
     6361 gcgccgtgat acaacgcctc atccagcttc tgctcactga atgtttgcgg attattcaac
     6421 gacggctcgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg
     6481 gcgaccccgg cgagggcgat aaagccagtg cccgtcgcca cggaaagatg aaagcccatc
     6541 caccacagca accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac
     6601 gcttcgcccc cccgacggaa cgccagatac aacagcacga agatgatcat cagcgtcatc
     6661 ggcaccatga gtttcagctt atggttggcg cgctccagca actcgaactg cccggaaaac
     6721 gccacgctgg tgcctggttt taactgcact ttttcagcta tcgctttttg caaatcgtga
     6781 accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca
     6841 ttctcggttt tcagcatcga cggtccggta gagactttaa cgtcggccac gtctgccagg
     6901 gtgatttgct gcttcatcgg cgtcaggatc ggcaactggc gcagtgcctg cggactatcg
     6961 cgccagcttt gcggataacg cagattaatt ggataacggg caatcccttc caccgtttcg
     7021 ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata
     7081 ccgtaacgtg cggctttttc acggttaatc tcaacgttga tatagcgccc accttccagc
     7141 cgctcggcaa gcgcagaagc tacgcctggc accgttcgtg ccacttcttc aatttgctcc
     7201 gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta
     7261 atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc
     7321 cccggcagac gcacggtgtt atccagttcc tcaatgattt tgtccatcgt catgcctggc
     7381 cgccactgct cctgcggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa
     7441 tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc
     7501 ataattagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc
     7561 agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca
     7621 actttattga gcggccagag aaccgtcagc accgaaagcg ctgccaccaa cagcgtggtt
     7681 ttcggccagt gcagtacttt cagcaataga ggatgataaa cacgaatcaa aaagcgattg
     7741 agcgggttac tgctttccgg cggaattttg ccacggatcc agtagcccat caggatcggg
     7801 atcactacga tcgccagcag cgccgcgccc gccatcgcat aggttttagt gaacgccagc
     7861 gggccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg
     7921 ataatcagca gactgataaa caacgccgga cccacttcaa cagacgcatc ggtgatcacc
     7981 tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc
     8041 cgtttatgcg cattctcgat catgacgata gcagcatcga ccatcgcccc gacggcaatc
     8101 gcaatgccac ccaacgacat aatattggca ttcagtccct ggaagtgcat gacaataaaa
     8161 gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag
     8221 agaaacagcg cacagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg
     8281 ttgtcgatgg cgcggtcaat gagctggctg cgatcgtatg ttgtaactat ctccacgcct
     8341 tccggcagac tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttcacgg
     8401 gcgtttttgc cggagcgcag gatcaccacc ccgcctgcca cttcgccttc gccgttcagt
     8461 tcggcaatgc cccggcgcat ttccggtcca atctggacct tcgcaacatc acgcagataa
     8521 acaggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgc
     8581 agatagccgc tggcgcgcac catatattcc gcttccgcca gttcgatcga cgaaccgccc
     8641 gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg ctagactgat gccatactgt
     8701 gccaggcgct gaggatcgat aaccacctga tactctttca ccacaccgcc caccgacgcc
     8761 acttccgcaa cgtcagggat ggtttttagc tcatatttga gaaaccagtc ctgcaatgag
     8821 cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag
     8881 ccaacacccg tggcatctgg ccccagctcg gcgctgactc ccgcaggcag cttaccctgt
     8941 acctggttga ggtattccag cacccgcgaa cgcgcccagt acggatcggt accatcttcg
     9001 aaaatgacat acacataaga gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca
     9061 ggcaccgaca acatggtggt ggttagcgga taagtcacct gattttcaac gatttgcggt
     9121 gcctggccgg gatagctggt tttaataatc acctgcacat cggagagatc cggcagcgca
     9181 tccactggcg tgttaatgat ggtccaggtg ccccagatac tcagaaataa cgcgcccatc
     9241 agcaccagaa aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc
     9301 ctcaatgcgc atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg
     9361 cttcggaatc aatcaggaac aggccgctgg aaaccacctt ttcaccttcc gccagaccag
     9421 agcgtaatgc ggtgacacct tgcgatgcct ggaaaacggc aacgcgtttc ggtacaaagc
     9481 gcccgtcggc atcaacggta atcacccgct gttcgttgcc ggtatcaatc agcgcctgtg
     9541 acggaatgag cagcatcggt tcgctggcgg tattgagttg caaccaggcg ttcatgcctg
     9601 gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag ttgtagcgtg cgggtcgcgg
     9661 catccacgcc gggtagcagg gtccatttgc ggatggtgag tgttttatca ggtcgcgccg
     9721 gaacggtgag cgtaaactgc gaggcatctt tcaccagcca ggcgatggac tccgggatcg
     9781 cagcagtgac ccacaccggg tccataccct gaattttcgc gaccacgtta tctttggcga
     9841 tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg
     9901 taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatccgcc tccggcattc
     9961 ccgccagtcg cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc
    10021 gcagcagtaa atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg
    10081 gtgtgccctt ttgcacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg
    10141 cagcgcgagc ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac
    10201 tctgggcaaa agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg
    10261 tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca
    10321 ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca
    10381 tcgggtcgta ccagaataag actttacgtt ctgcggtcga cgttttttct gcgggcggtt
    10441 cagcctttgc aaaccagtta aaacctgccg cagaaataat accgcccgcg atcatgctgc
    10501 cgataataag cgcgattttt ttcatatcat taaacctggg ttactggctg actttaatat
    10561 cctgtaataa agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt
    10621 taatttcact cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt
    10681 tcacggcagc aatcggatcg tgatggatgg tgattttttt gctttccaga tcaacacctt
    10741 ttaccacgcc agtggcgcta ataacctgcg gttgtacttc gctcatggtt tcatgatgat
    10801 gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca
    10861 gtgctttttt catttattct ctcctggagt taaaaattac tactgccaac cgccaccaag
    10921 tgcggtatat aaagaaattt cgttgacctg acgggcataa ttcaggtcaa gtaaggtttg
    10981 tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta ccgcgccgtg
    11041 ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gacgccagat aacgctgctg
    11101 ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca cttctttaaa
    11161 ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa tttcggcgat
    11221 atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt tgggtataaa
    11281 attccacatc ccgctgctgg cgttaaataa tgacgacaga tcgctgctgg cggtcgatat
    11341 tccgctggtc aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa tattggcatt
    11401 agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga
    11461 cgacaagccc gccggtaatt taacgctttg cagactgtcg ctgtttactg tctgcgcttg
    11521 cggcagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct gcgccagttc
    11581 cccctgacgt ttagcgatgt cactgcgggt actttctatc accccgcgag cctgttccag
    11641 cgccagaaca ttgctgctac cggtcaacag ttgtttttcg acaaacgcat atgactgctg
    11701 ataattacgc agcgtttctt cggctatttg caattgcgca tacgccagtt gctgattgaa
    11761 atagctttgc gcgacattag aaaccagcag aatatgcacc gcgcgctgag cttcctcagt
    11821 ggctaaataa ttttgtcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag
    11881 gtcaaggctg gcgttaaggc cggtcgaaaa ctcccgtgtc gtggcggtat tccctttaag
    11941 attaccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt cggcatcagt
    12001 cagacgatat tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt
    12061 attcaccagc gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca
    12121 gcccgcgttc tgatagttat ctgctgcgtt aacgaggccg ttctggctga gtgagaactg
    12181 ctgcggtacg ggcattgccg ggcgctgata atccggtgcc agtgaacaac cggttagcgc
    12241 aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat
    12301 tttatgccgc caactttact cgccagcctc tgattttccg gtgacaggaa aatgacaaaa
    12361 ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca
    12421 acatgtggag gaaatatgaa actgctgatt gtcgaagatg aaaagaaaac cggagaatac
    12481 ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat
    12541 ggctaccatc tggcgatgac cggagattat gacctgataa tcctcgatat tatgctgccg
    12601 gacgtgaacg gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt
    12661 ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggcc
    12721 gatgactatc tggtgaagcc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc
    12781 ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc
    12841 gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac cagtaaagaa
    12901 tttactctgc tggagttttt ccttcgccat cagggcgaag tgctgccccg ctcgcttatc
    12961 gcctcgcagg tatgggacat gaatttcgac agcgacacta acgccattga tgtggcggtg
    13021 aagcggctac gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc
    13081 ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc
    13141 gctggcaacc cgcctgacct tttttatcag cctggccacc atcgcggcgt ttttcgcctt
    13201 tgcatggatc atgatccact cagtaaaagt gcattttgcc gagcaggata ttaatgattt
    13261 aaaagagatt agcgccaccc ttgaacgggt actaaatcac cctgacgaaa cgcaagcccg
    13321 acgcttaatg acgctggaag atatcgtcag tggttattcc aatgtgttga tttccctggc
    13381 agatagtcag ggtaaaacgg tgtatcactc ccccggtgcg cccgatatcc gcgagtttac
    13441 gcgtgacgcc atacccgata aagacgcgcg gggcggcgag gtgtatctcc tttccggccc
    13501 gacgattatg atgccagggc acggtcacgg gcatatggaa cacagcaact ggcggatgat
    13561 taacttaccg gttggcccgc tggttgacgg caaaccgatt tatacgctct acatcgcgct
    13621 ttcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc
    13681 atcggtaatc agcatcctga tcgtctttat cgtgctgttg gcggtacata aaggtcacgc
    13741 gccgatccgc agcgtcagcc gtcaaatcca gaatattacc tcgaaagatc tcgacgttcg
    13801 ccttgacccg cagaccgtgc ccattgagct ggaacagctg gtactgtcgt tcaaccatat
    13861 gatcgagcgt attgaggatg tctttacccg ccagtccaat ttctcagcgg atatcgctca
    13921 cgaaattcgc acaccaatta cgaatctcat aacgcaaacc gaaatcgccc tcagccagtc
    13981 gcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat
    14041 ggcgaaaatg gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc
    14101 cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc
    14161 gttagcggaa gatcgcggcg tggagttgcg gtttgttggc gacaagtgtc aggtcgcggg
    14221 cgatccgctg atgctgcgtc gggcgctaag caacctgctt tcgaacgccc tgcgttatac
    14281 gccaaccgga gagacaattg tagtgcgctg tcagacggtc gatcacctgg tgcaagttat
    14341 cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt
    14401 ctatcgcgtt gacccctccc gccagcgaaa aggtgaaggt agcggtattg gcctggcgat
    14461 agtgaaatcg attgttgtcg cgcataaagg cacggttgcg gtaacgtcgg atgcgcgggg
    14521 gacaaggttt gttatcacat tacccgctta atccttcagc aaacggcaac ttttataacc
    14581 agtgtaaaaa taacgtgccg caatagtcat acctattaat ggtaaaaagc tgtcacaatt
    14641 cataaaaaac cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt
    14701 gtggactggc ttcttgatgt ttttgctacc tggctctacg gcttaaaagt aatcgcgata
    14761 acgttagcgg tcatcatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac
    14821 tgggtacgcc gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac
    14881 cgcgaactgt ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa
    14941 acgggcgtca tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat
    15001 catatctttg ttggcaccta ccccaacgac cccgatactc agcgtgatgt tgacgaagtg
    15061 tgcgctcgct tcccgaatgt gcataaggta gtctgcgcgc gtcctggccc caccagcaaa
    15121 gccgactgtc tgaacaacgt gctggacgcc atcacccaat ttgagcgtag cgccaatttc
    15181 gcttttgctg gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt
    15241 ctgttcaact atctggtcga gcgtaaagat ctgattcaga tcccggtgta tccgttcgaa
    15301 cgcgaatgga cgcacttcac cagcatgact tacattgatg agttttcaga gctgcatggc
    15361 aaagatgttc cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc
    15421 tgtttcagcc gccgcgccgt gaccgcactg ttagctgacg gtgacggtat tgctttcgac
    15481 gtgcagagtc ttactgaaga ttacgacatt ggcttccgcc tgaaagaaaa aggtatgacg
    15541 gaaatttttg tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt
    15601 ttacagcacg cgcggacatc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt
    15661 tcgactgcgg ttcgacaaaa atcccgctgg atcatcggca ttgttttcca aggctttaaa
    15721 acccataaat ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg
    15781 gcaatcagta actttgtcag cttcctcgcg atgctggtga tgatccagct tttgctgttg
    15841 ctggcgtatg aaagtttgtg gcccgatgcc tggcatttcc tttctatttt cagcggcagc
    15901 gcatggttaa tgaccctgct gtggctaaac tttggtttga tggttaaccg catcgtgcag
    15961 cgggtgattt tcgttactgg ctactacggc ctgacgcagg ggctgctttc cgtcctgcgt
    16021 cttttctggg gcaacctgat taacttcatg gccaactggc gcgcgctaaa acaggtactt
    16081 caacacggcg atccacgtcg cgtggcgtgg gataaaacaa cgcatgactt ccccagcgtg
    16141 actggcgata cccgctcgtt gcgcccgtta ggtcaaattc tgctggaaaa tcaggtcatc
    16201 actgaagaac aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt
    16261 tcaatgctga tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa
    16321 aacggcgtgg cgtgggaatc catcgatgcc tggcagatcc cttcctcgct gattgccgaa
    16381 atgccggcct ccgtggcgct gcattatgcg gtactgccgc tgcgtctgga aaatgacgag
    16441 ttaattgtcg gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa
    16501 gtcggacgca aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac agggttacgt
    16561 cactggtatg cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag
    16621 catcagtggc tcacggaaca gcaggccggt gaaatctggc ggcaatatgt gccgcatcag
    16681 ttcctgttcg ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg
    16741 ttgttattgc gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc
    16801 gttatcagcc aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg
    16861 atgcaatcac tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc
    16921 gaaaatgaag gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc
    16981 tttattgagt accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct
    17041 gagcgattat cgccattttg ttatttatcc ccgtctcgat aaagcgctga aggcacagaa
    17101 aaataacgac gaagcaaccg ccatccgcga atttgaatat atacaccagc aggtgccgga
    17161 taatattccg ctgactttat accttgcgga agcctatcgc cattttggtc atgatgaccg
    17221 ggcgcggctg ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg
    17281 cagtctggcg gctattccgg ttgaagtgaa aagcgttacg acagttgaag aactgcttgc
    17341 ccagcaaaaa gcgtgcgatg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa
    17401 tgccctgcgg ctggcacagt tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc
    17461 tgcatcgccg gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa tctacctgaa
    17521 acaatggtcc caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc
    17581 ggcagaacgc cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct
    17641 ggcactgcaa tcacagggga tcttcaccga tcctcagtca tatattactt acgcgaccgc
    17701 gctggcttat cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa ataagccact
    17761 atttaccacg gacgcacaag agaaaagttg gctctatctg ttatctaaat acagcgctaa
    17821 ccccgttcag gcgttggcga attatacggt acagtttgcc gacaaccgcc agtatgttgt
    17881 tggcgcgacg ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct
    17941 cgccaccctc cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg
    18001 taacaaggct gaagctctgc gtctggcacg attgctgtat cagcaagaac cggcaaatct
    18061 tacccgcctg gatcaactaa cctggcaact gatgcagaac gagcagtcac gcgaagctgc
    18121 cgatttattg ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agacattaat
    18181 ggcgcgactg gcgtctctgc tggaaagtca tccttacctg gcaacgccgg cgaaggtggc
    18241 gattttatcg aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg
    18301 tattgcagat aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga
    18361 tgccgccgcc tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgtt
    18421 gtatgcatgg cttcaggccg aacaacgaca accgagcgcc tggcaacatc gtgcggtagc
    18481 ctatcaggcg tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag
    18541 tcttcacgac atgagcaatg aggatctgct tgctgctgcc aataccgccc aggcggcagg
    18601 aaatggtgcg gctcgcgatc gctggctaca acaggcagaa aaacgtggac tgggaagcaa
    18661 tgccctctac tggtggctgc atgcgcaacg ttacattcct ggtcagccgg aactcgcact
    18721 gaacgatctc acgcgctcaa tcaatattgc gccttctgcc aacgcttacg ttgcgcgggc
    18781 gacaatttat cgccaacgtc ataatgtccc ggccgcggtg agtgatttgc gcgccgcgct
    18841 ggaactggaa ccgaataata gcaacaccca ggcagcgctt ggttacgcct tgtgggatag
    18901 cggtgatatc gcacagtcgc gggaaatgct cgaaccggcg cataaagggc ttccggacga
    18961 tccggcactg atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac
    19021 gcagcactac gcccggctgg tgattgatga cattgataat caggcgctga taaccccact
    19081 gaccccagaa caaaatcaac aacgcttcaa tttccgccgt ttgcatgagg aggtcggtcg
    19141 ccgctggacg ttcagtttcg attcttccat cggcttgcgt tccggcgcaa tgagtaccgc
    19201 taacaataat gtcggcggcg cagcgccagg gaaaagctat cgtagctacg gacaactgga
    19261 agccgagtac cgcatcggac gcaatatgct gctggaaggc gacctgctct cagtttatag
    19321 ccgcgtcttt gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa atccgatgtc
    19381 cggcaccggt ctgcgctgga agccgctgcg cgatcagatc tttttcatcg ccgtcgaaca
    19441 gcagttgccg ctgaacggcc aaaatggcgc atccgatacc atgctgcgcg ccagcgcctc
    19501 attctttaat ggcggcaaat acagcgacga atggcacccg aacggttcag gctggtttgc
    19561 ccaaaacctg tacctcgatg cggcgcaata tatccgccag gatattcagg cgtggacggc
    19621 agattatcgc gtcagctggc atcagaaggt agctaacgga cagactattg agccttacgc
    19681 tcacgttcag gacaacggct atcgtgataa aggcactcag ggcgcgcagc ttggcggagt
    19741 cggggtccgc tggaatatct ggaccggcga gacgcactac gacgcctggc cgcacaaagt
    19801 cagtctcggc gtcgagtatc aacatacctt taaggcgatt aatcaacgta acggagagcg
    19861 caacaacgcg tttctcacca ttggagtgca ctggtaaatg cgtaagttca ttttcgtatt
    19921 gctgacactg cttttggtca gccctttttc ctttgcgatg aaaggtatta tctggcaacc
    19981 acaaaaccga gatagtcagg ttaccgatac ccagtggcag gggctgatga gtcagttacg
    20041 tttgcaaggc ttcgataccc ttgttttgca atggacccgt tacggcgatg catttaccca
    20101 gccagaacag cgcacgttat tgtttaagcg ggccgcagct gcgcaacagg ctggtctgaa
    20161 gcttattgtc gggctgaacg ccgatccgga attttttatg caccagaaac agtcgtccgc
    20221 agcgctggaa agctatctta atcgcctgct ggctgccgat ctccagcaag ccagattatg
    20281 gagcgccgcg cctggcataa cgccggatgg ctggtacatc agcgcggaaa ttgacgacct
    20341 gaactggcgc agcgaagccg cccgtcagcc tttgctaaca tggttaaaca acgcgcagcg
    20401 gctgattagc gatgtttcag caaaaccggt ttatatcagt agttttttcg ccggaaacat
    20461 gtcgcccgat ggctatcgcc aactgctgga acacgttaaa gcaaccggcg ttaatgtctg
    20521 ggtacaggat ggcagcggcg tggataaact gaccgctgaa cagcgtgaac gttatttaca
    20581 ggccagcgcc gattgccaaa gtcccgcccc tgccagcggc gttgtttatg aactttttgt
    20641 cgccggcaaa ggcaaaacct ttacagcgaa accgaaaccg gacgcagaaa ttgcctcgct
    20701 gttagcgaaa cgttcctctt gcggtaaaga cactctctat ttctctctgc gctatttgcc
    20761 cgtcgcgcac ggcattctcg agtattaaat ctcctccagg taagtcgggt acgacctggc
    20821 ttacctcttt cgctctcatc agaattccat caagattaat tgctaaaaag cgtgctaaag
    20881 aaagaagatc gttatcggcg aacgggccga tcactcatgc aggaagcata gatcctttga
    20941 gcggagtttc tgatatctga ggagtgcgaa atgcaattga gcagcagtga accttgcgtg
    21001 gtgatcctga ccgaaaaaga ggtagaggta agcgtcaata accatgctac gtttaccctt
    21061 ccgaaaaact acctggccgc cttcgcgtgc aacaataacg tcattgaact ctcaacgtta
    21121 aatcacgtat taatcaccca catcaaccgt aacatcatca acgattatct gttgttttta
    21181 aataagaact taacctgtgt aaagccctgg tcgcggctgg caaccccggt tatcgcttgt
    21241 catagccgta caccggaagt gttccggcta gccgccaacc acagcaagca gcaacccagc
    21301 agaccctgcg aagcggagtt gacgcgcgca ttgcttttta ccgtattgtc taactttctt
    21361 gagcaatcgc ggtttattgc cctactgatg tatatcttac gcagcagcgt ccgcgacagc
    21421 gtctgccgca ttattcaaag cgatattcag cattactgga atctgcgaat tgtcgccagt
    21481 tcgctatgtt taagccccag cctgctcaaa aagaaattaa aaaacgaaaa taccagctat
    21541 agccagattg tcacagagtg tcgtatgcgt tacgccgtac agatgttatt gatggataac
    21601 aaaaatatca ctcaggtggc gcaattatgt ggctatagca gcacgtcgta ctttatctct
    21661 gtttttaagg cgttttacgg cctgacaccg ttgaattatc tcgccaaaca gcgacaaaaa
    21721 gtgatgtggt gaagggcaaa gcggaaacgg ataagacggg cataaatgag gaagaaatgg
    21781 cgcgccctgc aggattcgaa cctgcggccc acgacttaga aggtcgttgc tctatccaac
    21841 tgagctaagg gcgcgttgat accgcaatgc ggtgtaatcg cgtgaattat acggtcaacc
    21901 cttgctgagt caatggcttt tgatctggtt gctgaacaag tgaacgaccg cgtctgattt
    21961 tctgatttat ttcgctatag cggcaaacaa acgcacaccg ctgcgcgtct gaatcaagaa
    22021 aacccgtatt ttcatgtatc aaagtacaat ttcccgacct aacggaaaat tgtccgctcc
    22081 tatgagactg gtaactatga aaccaacgtc ggtgatcatt atggatactc atcctatcat
    22141 cagaatgtct attgaagttc tgttgcaaaa aaacagtgaa ttgcagattg tcctgaaaac
    22201 ggatgattat cgcataacca tcgattatct ccgaacccgt cctgttgatt taatcattat
    22261 ggatatagac ttgcccggaa cagacggttt taccttcctg aaaaggatca aacaaatcca
    22321 gagcacagtg aaagtgttat ttttatcatc gaaatcagaa tgcttttatg ctggcagagc
    22381 gatacaagct ggtgctaacg gttttgtcag taaatgcaat gatcagaatg atatttttca
    22441 tgccgttcag atgatcctct ccggatacac gttttttccc agcgaaacgc ttaactatat
    22501 aaaaagcaat aaatgtagta cgaatagttc aacggtcact gtgctatcta atcgtgaagt
    22561 gaccatatta cgttatctgg ttagcggatt atctaataaa gaaattgccg ataagttatt
    22621 acttagcaat aaaacagtta gtgcgcataa atctaatatt tatggcaagc taggtttgca
    22681 ttcaattgta gagctt
//
LOCUS       DBBGLQ010000067        22635 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.067, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000067 DBBGLQ010000000
VERSION     DBBGLQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.067"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(185..595)
                     /locus_tag="ACIYSB_003518"
     CDS             complement(185..595)
                     /locus_tag="ACIYSB_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HGC5430491.1"
                     /translation="MDQVVIFKQIFDKVRNDLNYQWFYSELKRHNVSHYIYYLATENV
                     HIVLKNDNTVLLKGLKNIVSVKFSKDRHLIETTSNKLKSREITFQEYRRNLAKAGVFR
                     WVTNIHEQKRYYYTFDNSLLFTESIQKTTQILPR"
     gene            complement(653..886)
                     /gene="ynfO"
                     /locus_tag="ACIYSB_003519"
     CDS             complement(653..886)
                     /gene="ynfO"
                     /locus_tag="ACIYSB_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGC5430492.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            1275..1820
                     /locus_tag="ACIYSB_003520"
     CDS             1275..1820
                     /locus_tag="ACIYSB_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGC5430493.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1795..3720
                     /locus_tag="ACIYSB_003521"
     CDS             1795..3720
                     /locus_tag="ACIYSB_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGC5430494.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESLHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3717..3923
                     /locus_tag="ACIYSB_003522"
     CDS             3717..3923
                     /locus_tag="ACIYSB_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGC5430495.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3920..5521
                     /locus_tag="ACIYSB_003523"
                     /pseudo
     CDS             3920..5521
                     /locus_tag="ACIYSB_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            5502..6821
                     /locus_tag="ACIYSB_003524"
     CDS             5502..6821
                     /locus_tag="ACIYSB_003524"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGC5430496.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6831..7163
                     /locus_tag="ACIYSB_003525"
     CDS             6831..7163
                     /locus_tag="ACIYSB_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGC5430497.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            7219..7464
                     /locus_tag="ACIYSB_003526"
                     /pseudo
     CDS             7219..7464
                     /locus_tag="ACIYSB_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            7627..8862
                     /locus_tag="ACIYSB_003527"
     CDS             7627..8862
                     /locus_tag="ACIYSB_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HGC5430498.1"
                     /translation="MAGENKLSDKALKGYLGKPREKQITIADGKGLSIRVSTKGAVSF
                     VFFYRLAGGRAAPVWLTLGKYPDMSLKQAREKRDECRGWLADKRDPRIQIKIQAEERL
                     KPVTVEDALNYWYENYCKVRRKTHAVTLGRFRKHIFPYIGHLPVNDTHLYEWLDCFDR
                     IKRNAPVMAAYVFSDTKLALRFCRVRQYATCDALKDLRMSDVGQIAGKRDRVLDEAEL
                     GQLWKAIFVEPDLKLMSEYTRKMFVLCTVFGCRMSEARLSEWSEWDLESWVWTVPKEH
                     SKTGVEIVRPVPEILRQWVTDVHEETKHTGYVLGSLRIRESVSKIGGKIGKRLGHEKQ
                     WSLHDLRRTLSTHLSDLGVEFYVVEQLLGHALPGVAGVYNRSKFMAKKLDALELWTTY
                     LNSIAGADSKVTILKQKAG"
     gene            8864..9094
                     /locus_tag="ACIYSB_003528"
     CDS             8864..9094
                     /locus_tag="ACIYSB_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5430499.1"
                     /translation="MKKMAIVDKKGLEYIPNIDRMIREKECRELTTLANSTRWKLEKE
                     GKFPKRIKIGSTAVAYRLSEVQAWIRGEWETT"
     gene            9111..9932
                     /locus_tag="ACIYSB_003529"
     CDS             9111..9932
                     /locus_tag="ACIYSB_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HGC5430500.1"
                     /translation="MAIFHYTDLFGLKGILDSNSLWATNIYFLNDKEESNHGCECFRN
                     TIKIVDDNIIPKDKKTILLKSLDMYEKGRLQKEKGIDKHVYSISFCKENDKLSQWRGY
                     GNKQGVCIEFDADELVSFSQNIYLNCVAHDVIYSNNNDVTKMSKELGAFFSCNGINIK
                     EKNDHFVTMASTYQFISKYIPFFKHPSFIEENEFRLVFTPRILIPDVQFRINNNGVIP
                     YIIIGNKDHRKLPIKSITIGPTNDYDFIEAGIKMFLDSKGFSSVEIKSSSIPFRG"
     gene            complement(10064..10420)
                     /locus_tag="ACIYSB_003530"
     CDS             complement(10064..10420)
                     /locus_tag="ACIYSB_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGC5430501.1"
                     /translation="MKYEFFATSSSSLDVYIVTVSNDSSSLIMTCNCPAGSKGILCRH
                     RKALITGKIRGIFTPPRRNNPEKLQEAINLIATYGIDKTLKLYTDELERAEQTWISVR
                     DNLRAMINALVEPPKY"
     gene            10449..10715
                     /locus_tag="ACIYSB_003531"
                     /pseudo
     CDS             10449..10715
                     /locus_tag="ACIYSB_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10838..10936
                     /locus_tag="ACIYSB_003532"
     CDS             10838..10936
                     /locus_tag="ACIYSB_003532"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGC5430502.1"
                     /translation="MEAASEQEARQQSPTGCVMIFAARIRQEVCRA"
     gene            10929..11114
                     /locus_tag="ACIYSB_003533"
     CDS             10929..11114
                     /locus_tag="ACIYSB_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430503.1"
                     /translation="MLKTFRVFARAVNPLGHTIGIAQNVKAVNVQTAIAAVRSESSEY
                     GLSQVIISEVYELKEVH"
     gene            11114..11305
                     /locus_tag="ACIYSB_003534"
     CDS             11114..11305
                     /locus_tag="ACIYSB_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430504.1"
                     /translation="MQEITLHEAAERAHQTEIICRLLEVYPNKITDADISALASLLAR
                     LSGSVASFLIEEESKLVGD"
     gene            11306..11527
                     /locus_tag="ACIYSB_003535"
     CDS             11306..11527
                     /locus_tag="ACIYSB_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430505.1"
                     /translation="MNTEREVFFKLLACAESSLTLNNSAKAILNMWLDCINDNEDANI
                     AYGLLSLIDEAAEKLNDAINSALLSNKSS"
     gene            11545..11844
                     /locus_tag="ACIYSB_003536"
     CDS             11545..11844
                     /locus_tag="ACIYSB_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGC5430506.1"
                     /translation="MEMKNSGFIASGPARPEFMNGDIYRDKYGGTVTIKGVAERRITY
                     RREGYSYDCVMPVYQFRRDFSLVYAAPRSKPISREKARGNIQKMKSMINAFRGKK"
     gene            11988..13592
                     /locus_tag="ACIYSB_003537"
     CDS             11988..13592
                     /locus_tag="ACIYSB_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HGC5430507.1"
                     /translation="MLADEQLKNITDYRIIDEDRQCVRVYRAGHITEHSMTQIVTLLA
                     VAGVKTVHEYAGITDTSPVDLSDQLPRLKEECERGESLVLNLPTKQKAQLSQMADSER
                     AQLLADRFDGVCVHAESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINN
                     AVEALKVIAAPMGEPSGDLLPFTNGVLNLKTGEFSPHSPEHWSTTHNGIEYTPPVAGE
                     NIRDNAPNFHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTF
                     THIATLLAGKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGD
                     PVEINPKYEKRFTTIIRAVVLATNNDPMIFTERAGGVSRRRVIFRFDNIVREDEKDKE
                     LPEKIAAEIPVIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVVELCAALEF
                     LEEARGLMMGGGGDTVKYTTRNSLYRVYMAFMAYTGKGKCLSVNEFGKAMRSAAKVYG
                     YEYITRKVKGVTQTNATTTDDCDAFL"
     gene            13873..14115
                     /locus_tag="ACIYSB_003538"
     CDS             13873..14115
                     /locus_tag="ACIYSB_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430508.1"
                     /translation="MHTSGKLNKHIKPHYRALDMAEHWLRVAIKAIDRNAGEGYAKAH
                     PELISAFMTTAAANFATLTEREIAEAEEVTTINIKS"
     gene            14127..14576
                     /locus_tag="ACIYSB_003539"
                     /pseudo
     CDS             14127..14576
                     /locus_tag="ACIYSB_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            14793..15833
                     /locus_tag="ACIYSB_003540"
     CDS             14793..15833
                     /locus_tag="ACIYSB_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGC5430509.1"
                     /translation="MVDLYSPTQLVQVVNAVDVQKQLNALFTSLFFTRSVMFESRDII
                     LDTIDDPNIPIAAFCSPMVGSKVSRDEGYESKTIRPGYMKPKSSIDPNKLAVRPAGVS
                     PEQYNAFGARNIKVKQAIVNQAKAIRARIEWLAVQAITTGKNIIEGDGIERYELDWNI
                     KPQNIITQSGGTEWSSKDKETFDPNDDIESYAEFSEGVTNIIIMGGNVWKKYRSFRAI
                     KEALDTRRGSNSELETALKDLGDSVSFKGYMGDVAIVVYSGRYTDEDGTEKHFLDPDL
                     MVLGNTALQGIVAYGGIQDPELIRMGLTKAELAPKNYIVPGDPAIEYVQTHSAPQPIP
                     ARINRFVTVRIG"
     gene            15843..16184
                     /locus_tag="ACIYSB_003541"
     CDS             15843..16184
                     /locus_tag="ACIYSB_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGC5430510.1"
                     /translation="MATHYTELMAGTEALVTTLGIFSANKGVIPAFTPLMQEDATGAL
                     VVWDGSSVGKAVYVSAVQIDTAKKTRAQVYKTGVLNVDALNWPESVKELSVKIAAFVG
                     SGISVQPLARV"
     gene            16196..16579
                     /locus_tag="ACIYSB_003542"
     CDS             16196..16579
                     /locus_tag="ACIYSB_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5430511.1"
                     /translation="MQNDYNDLKPIAEMMYPNPAVEELKAIADKMCLSERLVDMNQVM
                     EITTLSRRTLLNLEASGEFPERVQVTEGRKAWYLSEVIDWINNIPRASEYCRVPVPKK
                     PDAALCLKIERVRRNARDGRYKLIG"
     gene            16825..17376
                     /locus_tag="ACIYSB_003543"
                     /pseudo
     CDS             16825..17376
                     /locus_tag="ACIYSB_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231112.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            complement(17483..18241)
                     /locus_tag="ACIYSB_003544"
     CDS             complement(17483..18241)
                     /locus_tag="ACIYSB_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HGC5430512.1"
                     /translation="MPGINEINIGKALARKLIAELITPREDIQNLNPRIRTMLSDFQK
                     SFPLGIPAGLIERFQEMVNIPPVGIGAPAAIQEMKDVWRGMLNNRRILCLSESPIITP
                     MWNHYADKYKGIVIEFDCVDFLDSAWLIAKAMKYTDKMPLTHTAEGMAELLFMPDNKS
                     IEYINNEIIFIKTEEWAYEREWRISTYARPNSTGQYSDLKFHPFELKSVILGPLFDAS
                     EFDEVASLVKMYPRAKLFKSSFGNDRKISIVPLP"
     gene            18525..19313
                     /locus_tag="ACIYSB_003545"
                     /pseudo
     CDS             18525..19313
                     /locus_tag="ACIYSB_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            19355..19753
                     /locus_tag="ACIYSB_003546"
     CDS             19355..19753
                     /locus_tag="ACIYSB_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGC5430513.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            19765..20118
                     /locus_tag="ACIYSB_003547"
     CDS             19765..20118
                     /locus_tag="ACIYSB_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGC5430514.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            20130..20708
                     /locus_tag="ACIYSB_003548"
     CDS             20130..20708
                     /locus_tag="ACIYSB_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5430515.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            20705..21100
                     /locus_tag="ACIYSB_003549"
     CDS             20705..21100
                     /locus_tag="ACIYSB_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGC5430516.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            21108..21848
                     /locus_tag="ACIYSB_003550"
     CDS             21108..21848
                     /locus_tag="ACIYSB_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5430517.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            21864..22286
                     /locus_tag="ACIYSB_003551"
     CDS             21864..22286
                     /locus_tag="ACIYSB_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGC5430518.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            22313..>22635
                     /locus_tag="ACIYSB_003552"
     CDS             22313..>22635
                     /locus_tag="ACIYSB_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGC5430519.1"
                     /translation="MGRPDWRAMLAGMSSTEYADWHRFYSTHYFHDVLLDMHFSGLTY
                     TVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAGGVRFGPDGNEVIPAS
                     PDVAD"
BASE COUNT         5867 a         5075 c         6168 g         5525 t
ORIGIN      
        1 gaaagctatg atgtcaagat taatttaatc tgatgtattc aataataaaa tttatccata
       61 aacctcgttt ttacggggtt ttgttatatt tgaatggttc cgaatatcta aatcacaatt
      121 gttgatggtt tttattaaac caatgcagtc cggctcagga gtgagagaag ccggacgtta
      181 ttgtttagcg tggtaagatc tgtgtagttt tctggatgct ttcagtaaat agtaatgaat
      241 tatcaaaggt atagtaatat cttttttgtt cgtggatatt tgtaacccac cgaaaaactc
      301 ctgctttagc aaggtttctt ctgtattcct gaaatgtgat ctctctggat ttcagcttat
      361 tagaggtcgt ttctataaga tgcctatcct ttgaaaattt gacagacaca atgtttttta
      421 ggccctttaa taacactgta ttatcatttt ttaatacaat atgaacattc tctgtggcta
      481 aatagtaaat gtaatgtgag acattgtgac gttttagctc agaataaaac cattgatagt
      541 ttaaatcgtt tcgaacttta tcaaatattt gtttaaaaat gactacctga tccatagata
      601 aaccttccat gtgatatgag ggggcgtagt ctgcacgatt atctaaattg cttcaatctg
      661 gtctgacctg ttttctgagc aattcagtaa tgtcactctt ttctttgttt gcttcagaag
      721 aaactctttt ttctgagcac agtctccggc ggcaggcttc aatcacccag gctgagaaat
      781 tcccggaccc tttttgctca agagcgatgt taatttgttc aatcatttgg ttaggaaagc
      841 ggatgttgcg ggttgttgtt ctgcgggttc tgttcttcgt tgacatgagg ttgccccgta
      901 ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga tgcagatcaa
      961 ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcc tccacgcacg
     1021 ttgtgatatg tagatgataa tcattatcac tttacgggtc ctttccggtg atccgacagg
     1081 ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg tttaaggcat
     1141 ttccgttctt cttcgtcgta acttaatgtt tttatttaaa ataccccctg aaaagaaagg
     1201 aaacgacagg tgctgaaaac gagcttttgg gcctctgtcg tttcctttct ctgtttttgg
     1261 ccgtggaatg aacaatggaa gtcaacaaaa agcagctggc tgacattttc ggtgcgagta
     1321 tccgtaccat tcagaactgg caggaacagg gaatgcccgt tctgcgaggc ggtggcaagg
     1381 gtaatgaggt gctttatgac tctgccgccg ttataagatg gtatgccgaa agggatgctg
     1441 aaattgagaa cgaaaagctg cgccgggaag ttgaagaact gcggcaggcc agcgagacag
     1501 atctccagcc agggactatt gagtacgaac gccatcgact tacgcgtgcg caggccgacg
     1561 cacaggagct gaaaaatgcc agagactccg ctgaagtggt ggaaaccgca ttctgtactt
     1621 tcgtgctgtc gcggatcgca ggtgaaattg ccagtattct cgacgggatc cccctgtcgg
     1681 tgcagcggcg ttttccggaa ctggaaaacc gacatgttga tttcctgaaa cgggatatca
     1741 tcaaagccat gaacaaagca gccgcgctgg atgaactgat accggggttg ctgagtgaat
     1801 atatcgaaca gtcaggttaa caggctgcgg cattttgtcc gcgccgggct tcgctcactg
     1861 ttcaggccgg agccacagac cgccgttgaa tgggcggatg ctaattacta tctcccgaaa
     1921 gaatccgcat accaggaagg gcgctgggaa acactgccct ttcagcgggc catcatgaat
     1981 gcgatgggca gcgactacat ccgtgaggtg aatgtggtga agtctgcccg tgtcggttat
     2041 tccaaaatgc tgctgggtgt ttatgcctac tttatagagc ataagcagcg caacaccctt
     2101 atctggttgc cgacggatgg tgatgccgag aactttatga aaacccacgt tgagccgacc
     2161 atccgcgata ttccgtcgct gctggcgctg gctccgtggt atggcaaaaa gcaccgggat
     2221 aacacgctca ctatgaagcg tttttccaat ggtcgtggct tctggtgcct gggcggtaaa
     2281 gcggcaaaaa actaccgtga aaagtcggtg gatgtggcgg gttatgatga acttgctgcc
     2341 tttgatgagg atattgaaca ggaaggctct ccgacgttcc ttggcgacaa acgtattgaa
     2401 ggctcggtct ggccaaagtc catccgtggc tccacgccca aagtgagagg cacctgccag
     2461 attgagcgtg cagccagtga atccctgcat tttatgcgtt ttcatgttgc ctgcccgcac
     2521 tgcggggagg agcagtatct taaatttggc gacaaagaga cgccgtttgg cctcaaatgg
     2581 acgccggatg acccctccag cgtgttttat ctctgcgagc ataatgcctg cgtcatccgc
     2641 cagcaggagc tggactttac tgatgcccgt tatatctgcg aaaagaccgg gatctggacc
     2701 cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga ttgagccgcc ggacagtgtg
     2761 acctttcaca tctggacggc gtacagcccg ttcaccacct gggtgcagat tgtcaaagac
     2821 tggatgaaga cgaaagggga tacgggaaaa cgtaaaacct tcgtgaacac cacgctcggt
     2881 gagacatggg aagcgaaaat cggcgaacgt ccggatgctg aggtgatggc ggagcggaaa
     2941 gagcattatt cagcgcccgt tcctgaccgt gtggcttacc tgaccgccgg tatcgactcc
     3001 cagctggacc gctacgaaat gcgcgtatgg ggatgggggc cgggtgagga aagctggctg
     3061 attgaccggc agattattat gggtcgccac gacgatgaac agacgctgct gcgtgtggat
     3121 gaggccatca ataaaaccta tacccgccgg aatggtgcag aaatgtcggt atcccgtatc
     3181 tgctgggata ctggcgggat tgacccgacc attgtgtatg aacgctcgaa aaaacatggg
     3241 ctgttccggg tgatccccat taaaggggca tccgtctacg gaaagccggt ggccagcatg
     3301 ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa tcggtacgga taccgcgaaa
     3361 gagcagattt ataaccgctt cacactgacg ccggaagggg atgaaccgct tcccggtgcc
     3421 gttcacttcc cgaataaccc ggatattttt gatctgaccg aagcgcagca gctgactgct
     3481 gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa tactgtggga cagcaaaaag
     3541 cgacgcaatg aggcactcga ctgcttcgtt tatgcgctgg cggcgctgcg catcagtatt
     3601 tcccgctggc agctggatct cagtgcactg ctggcgagcc tgcaggaaga ggatggtgca
     3661 gcaaccaaca agaaaacact ggcagattac gcccgtgcct tatccggaga ggatgaatga
     3721 cgcgacagga agaacttgcc gctgcccgtg cggcactgca tgacctgatg acaggtaaac
     3781 gggtggcaac ggtacagaaa gacggacggc gagtggagtt tacggccact tccgtgtctg
     3841 acctgaaaaa atacattgcg gagctggagg tgcagaccgg catgacacag cgacgcaggg
     3901 gacctgcagg attttatgta tgaaaacgcc caccattccc acccttctgg gaccggacgg
     3961 catgacatcg ctgcgtgaat atgccggtta tcacggcggt ggcagcggat ttggtgggca
     4021 gttgcgggcg tggaacccac cgggtgaaag tgtggatgca gccctgctgc ccaactttac
     4081 ccgtggcaat gcccgcgcag acgatctggt acgcaataac ggctatgccg ccaacgccat
     4141 ccagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg
     4201 gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc
     4261 atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt
     4321 taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca
     4381 ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc
     4441 gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca
     4501 gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg
     4561 gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg cctcgttcat
     4621 tcacgttttt gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt tttacagcgt
     4681 gatggagcag atgaagatgc tcgacacgct gcagaacacg cagctgcaga gcgccattgt
     4741 gaaggcgatg tatgccgcca ccattgagag tgagctggat acgcagtcag cgatggattt
     4801 tattctgggc gcgaacagtc aggagcagcg ggaaaggctg accggctgga ttggtgaaat
     4861 tgccgcgtat tacgccgcag cgccggtccg gctgggaggc gcaaaagtac cgcacctgat
     4921 gccgggtgac tcactgaacc tgcagacggc tcaggatacg gataacggct actccgtgtt
     4981 tgagcagtca ctgctgcggt atatcgctgc cgggctgggt gtctcgtatg agcagctttc
     5041 ccggaattac gcccagatga gctactccac ggcacgggcc agtgcgaacg agtcgtgggc
     5101 gtactttatg gggcggcgaa aattcgtcgc atcccgtcag gcgagccaga tgtttctgtg
     5161 ctggctggaa gaggccatcg ttcgccgcgt ggtgacgtta ccttcaaaag cgcgcttcag
     5221 ttttcaggaa gcccgcagtg cctgggggaa ctgcgactgg ataggctccg gtcgtatggc
     5281 catcgatggt ctgaaagaag ttcaggaagc ggtgatgctg atagaagccg gactgagcac
     5341 ctacgagaaa gagtgcgcga aacgcggtga cgactatcag gaaatttttg cctagcaggt
     5401 ccgtgaaacg atggagcgcc gcgcagccgg tcttaaaccg cccgcctggg cggctgcagc
     5461 atttgaatcc gggctgcgac aatcaacaga ggaggagaag agtgacagca gagctgcgta
     5521 atctcccgca tattgccagc atggctttta atgagccgct gatgcttgaa cccgcctatg
     5581 cgcgggtttt cttttgtgcg cttgcaggcc agcttgggat cagccgcctg acggatgcgg
     5641 tgtccggcga cagcctgact gcccaggagg cactcgcgac gctggcatta tccggtgatg
     5701 atgacggacc acgacaggcc cgcagttatc aggtcatgaa cggcatcgcc gtgctgccgg
     5761 tgtccggcac gctggtcagc cggacgcggg cgctgcagcc gtactcgggg atgaccggtt
     5821 acaacggcat tatcgcccgt ctgcaacagg ctgccagcga tccgatggtg gacggcattc
     5881 tgctcgatat ggacacgccc ggcgggatgg tggcgggggc atttgactgc gctgacatca
     5941 tcgcccgtgt gcgtgacata aaaccggtat gggcgctggc caacgacatg aactgcagtg
     6001 caggtcagct gcttgccagt gccgcctccc ggcgtctggt cacgcagacc gcccggacag
     6061 gctccatcgg cgtcatgatg gctcacagta attacggtgc tgcgctggag aaacagggtg
     6121 tggaaatcac tctgatttac agcggcagcc ataaggtgga tggcaacccc tacagccatc
     6181 ttccggatga cgtccgggag acactgcagt cccggatgga cgcaacccgc cagatgtttg
     6241 cgcagaaggt gtcggcatat accggcctgt ccgtgcaggc tgtgctggat accgaggctg
     6301 cagtgtacag cggtcaggag gccattgatg ccggactggc tgatgaactt gttaacagca
     6361 ccgatgcgat caccgtcatg cgtgatgcac tggatgcacg taaatcccgt ctctcaggag
     6421 ggcgaatgac caaagagact caatcaacaa ctgtttcagc cactgcttcg caggctgacg
     6481 ttactgacgt ggtgccagcg acggagggcg aaaacgccag cgcggcgcag ccggacgtga
     6541 acgcgcagat caccgcagcg gttgcggcag aaaacagccg cattatgggg atcctcaact
     6601 gtgaggaggc tcacggacgc gaagaacagg cacgcgtgct ggcagaaacc cccggtatga
     6661 ccgtggaaac ggcccgccgc attctggccg cagcaccaca gagtgcacag gcgcgcagtg
     6721 acactgcgct ggatcgtctg atgcaggggg caccggcacc gctggctgca ggtaacccgg
     6781 catctgatgc cgttaacgat ttgctgaaca caccagtgta agggatgttt atgacgagca
     6841 aagaaacctt tacccattac cagccgctgg gcaacagtga cccggctcat accgcaaccg
     6901 cgcccggcgg attgagtgcg aaagcgcctg caatgacccc gctgatgctg gacacctcca
     6961 gccgtaagct ggttgcgtgg gatggcacca ccgacggtgc tgccgttggc attcttgcgg
     7021 ttgctgctga ccagaccagc accacgctga cgttctacaa gtccggcacg ttccgttatg
     7081 aggatgtgct ctggccggag gctgccagcg acgagacgaa aaaacggacc gcgtttgccg
     7141 gaacggcaat cagcatcgtt taactttacc cttcatcact aaaggccgcc tgtgcggctt
     7201 tttttacggg atttttttat gtcgatgtac acaaccgccc aactgctggc ggcaaatgag
     7261 cagaaattta agtttgatcc gctgtttctg cgtctctttt tccgtgagag ctatcccttc
     7321 accacggaga aagtctatct ctcacaaatt ccgggactgg taaacatggc gctgtacgtt
     7381 tcgccgattg tttccggtga ggttatccgt tcccgtggcg gctccacctc tgaatttacg
     7441 ccgggatatg tcaagccgaa gcacttagca tggctttctg aggctttcgt gtagttgctg
     7501 gtttttacac ttaattcttt gataataaag aataagatta tctggcgctt tcactggatt
     7561 ttcctcgtta tctgtgtgtt gcaatcatct ctgtattgca gcttgtattg ctttttgggg
     7621 ctaaaaatgg ctggcgagaa caaactgagc gacaaagcgc ttaaaggata tctggggaaa
     7681 cccagagaaa agcagatcac cattgctgat ggaaaggggc tttctattcg tgtgagtact
     7741 aagggggctg tgagctttgt tttcttctac aggttagcag gtggccgggc tgctccggtc
     7801 tggctaacgt tgggtaaata tcctgatatg tcactcaaac aggcaagaga aaagcgcgac
     7861 gagtgccgtg gttggttggc tgacaaacgt gatccgcgta tccagattaa gattcaggct
     7921 gaagaacgct taaagccggt cacagtggag gatgcactaa attactggta tgaaaattac
     7981 tgtaaggtgc gtcgtaaaac tcatgctgta acgcttggca gatttcgaaa gcatatcttt
     8041 ccctatatcg gtcatttgcc cgtaaatgac actcacctat atgaatggct ggactgtttt
     8101 gaccgaatta aacgtaatgc accagttatg gcggcgtatg ttttttctga cactaaatta
     8161 gctcttcgtt tttgtcgggt acgccagtac gcgacgtgtg atgctttaaa ggatttgcgc
     8221 atgagtgatg tggggcagat tgcaggtaag cgggatcggg ttctggatga agccgaactg
     8281 ggccagctct ggaaggcaat ttttgtcgag cctgatttaa aactaatgtc tgaatacacg
     8341 cgaaaaatgt ttgtgctttg tacagtattt ggatgtcgaa tgagtgaagc ccgattatca
     8401 gaatggagcg aatgggatct agaaagttgg gtttggactg taccaaaaga acactcaaaa
     8461 accggtgttg aaatcgtcag accagtacct gaaattctac gacagtgggt aacggatgtt
     8521 cacgaagaga caaaacatac tggttatgtg ctgggaagtc tgcgaattag agaaagcgta
     8581 agcaagattg gggggaaaat cggtaaacgt ttgggccatg aaaaacaatg gtcactacac
     8641 gaccttagaa gaacgctatc tactcatcta agtgatctcg gtgttgaatt ttatgtagta
     8701 gaacaactgt taggccatgc gctacctggc gtggcaggtg tttacaaccg gagtaagttt
     8761 atggctaaaa aactggatgc tctggaactc tggactacat atctcaatag catcgcaggt
     8821 gctgattcaa aagtgacaat cctcaaacaa aaggctggtt aacatgaaaa aaatggcaat
     8881 tgttgataaa aagggtctgg agtacattcc taacattgac cgaatgatcc gtgagaaaga
     8941 atgtcgggag ctaaccactc ttgcgaacag cacacgctgg aagctggaga aggaaggaaa
     9001 atttcctaag cggatcaaga ttggttccac tgctgttgca tatcgtcttt cagaagtgca
     9061 ggcatggatt cgaggtgagt gggaaacaac ttaactatta ggtgggcatt gtggctatat
     9121 ttcattatac agatcttttt ggtttgaaag gtatattaga ctctaattct ttatgggcta
     9181 caaacatata tttcttaaat gataaagagg agtctaacca tggatgtgaa tgctttcgaa
     9241 atacaattaa aatagttgat gacaatatca ttccaaaaga taagaaaact attttgctca
     9301 agtctcttga catgtatgag aaagggaggt tacaaaaaga aaaagggatt gataaacatg
     9361 tttatagcat ctctttttgt aaggaaaacg ataagttaag tcagtggaga ggttacggaa
     9421 ataaacaggg tgtttgcatt gagtttgatg cggatgaact tgtgagcttt tctcaaaata
     9481 tttatttaaa ttgtgttgca catgatgtta tatattcaaa taataacgat gttactaaga
     9541 tgagtaagga gttgggggcg tttttttctt gtaatggtat taatattaaa gaaaagaatg
     9601 atcattttgt aacaatggcc tctacgtatc aatttatatc taaatacatc ccatttttta
     9661 aacatcctag ttttatcgaa gaaaatgaat ttagattggt atttactccg cgcatactaa
     9721 taccagatgt gcaattcaga ataaataata atggtgtcat cccttatatc atcattggta
     9781 ataaagatca taggaagtta ccaattaaaa gtattactat tggcccgaca aacgattatg
     9841 atttcattga ggctggtata aaaatgttct tggattcaaa aggatttagt tcagtggaaa
     9901 ttaaatcctc atcaattcca ttcagggggt aaggattaac acttttaatc tatgatgtta
     9961 taaaaatggt ggataaaggc gaccagaatt ggtcgccaat gtgagtatat agttagcctt
    10021 aatcagaata cgaggttgtc gactgatagg caatgtgaac agttcaatat ttaggtggtt
    10081 caacaagagc gtttatcatt gctctcaaat tatcgcgaac acttatccaa gtctgttctg
    10141 ctcgttctaa ctcatcagtg tacaacttta atgttttatc tatgccatat gtggctatta
    10201 aatttatagc ttcttgtaac ttttcgggat tattccttct tgggggagtg aatataccac
    10261 ggatttttcc ggtaattaaa gctttacggt gtctacatag aataccttta cttcctgctg
    10321 gacaattgca agtcatgatt agcgagctag aatcgtttga tacagtgact atgtatacat
    10381 ccaaactact gctacttgtt gcgaagaatt cgtatttcat gtgttagctc cttgacttca
    10441 attactctta tccttttggt tcaataccgc gccgtagcag ctctttgcgc cctaattctt
    10501 taagccagtt ggctaggctt atgccgtcgc tctgcgcttc tttgtcgaat tgctctttta
    10561 gctctggaga aattcgcatt ctgaattgtg gagattgccc atcaccctta ggacttttat
    10621 cgcgtttgat tgttgacaag tggccaccta ttgaattagc ctttttactg ttaggtgacc
    10681 accttaacac ggaggcactt aaaaaagcaa agcccgaagg cgctaggaac accaacgggc
    10741 ttctaaccac caacgatagc aacagtatcg agatagctat gagaaatcat accacacacc
    10801 cgcaagggcg ggacccgcac aaccacgcgt aatacacatc gaggccgcca gcgaacagga
    10861 agcccgccaa caatccccaa ctggctgcgt gatgatattt gccgcccgta ttcgtcagga
    10921 ggtatgccgt gcttaaaacc ttccgtgtat ttgctcgtgc ggttaaccca ctaggccaca
    10981 caattggtat cgctcagaac gtgaaggctg ttaatgttca gacggctatt gctgcggtga
    11041 gaagcgaatc atcagaatat ggcttatcac aagtcattat ttcagaagtg tatgaattaa
    11101 aagaggtgca ttaatgcagg aaattacatt acacgaagcc gctgaacgtg cgcaccagac
    11161 agaaattatt tgccgccttc ttgaggtata cccgaacaaa attacagatg ctgatatatc
    11221 cgcactggcg agcctactgg cgcgtctttc gggaagtgtc gctagttttt tgattgagga
    11281 agaaagtaag ctggtggggg attaaatgaa tacagaacgg gaagtctttt ttaaattgtt
    11341 agcatgtgca gaaagttcat taactttaaa taattcagca aaagcaatat taaatatgtg
    11401 gcttgattgc ataaatgaca atgaagatgc aaatattgct tatggcctgt tgtcacttat
    11461 tgatgaagca gcagaaaaac tcaatgacgc aataaatagt gccctgctat caaataagtc
    11521 gagttaagtc gaggaataaa taatatggaa atgaaaaatt ctggctttat tgccagcggc
    11581 cccgctcggc ctgaatttat gaacggcgat atttaccgcg ataaatacgg cggcacggta
    11641 acgattaaag gcgtggcaga acggcgcatc acttaccgtc gtgaggggta tagctatgac
    11701 tgcgtgatgc ctgtttatca gttccggcgt gatttttccc tggtatatgc cgcaccccgc
    11761 agtaagccca tcagcaggga aaaagcgcgg ggaaacatcc agaaaatgaa aagcatgatt
    11821 aacgcattca ggggcaaaaa atgaaactgg caccgaactt aaaaaaacag ccacgcgaca
    11881 gactgacaga ggtaatcatc tttgcaggta gtgatgcgtg gagccatgcg aaagagtggt
    11941 aggaatgggc gggtaaacat attgccgccg acgatgtgcc gcctgtcgtg ttggctgatg
    12001 agcaactgaa aaacatcacc gattaccgga tcattgatga agatcgtcag tgtgtgcgtg
    12061 tttaccgcgc aggacatatc acagagcaca gcatgacgca gattgttacg ttactggctg
    12121 tggctggagt gaagaccgta cacgaatacg cggggattac tgacaccagc ccagtggatt
    12181 tatccgacca gttgccgcga ctcaaagagg aatgcgagcg tggggaaagc ctggtgctta
    12241 atcttccgac gaagcaaaag gcgcaacttt cacagatggc agacagtgag cgggcacaac
    12301 tacttgccga tcgctttgat ggtgtttgtg ttcatgcaga aagtgaaatc gtccacgtat
    12361 ggcgcggcgg ggtatggtgt ccggtcagca caatggagct gagccgcgaa atggtggcga
    12421 tctattcaga gcacagggcc acgttcagca agcgcgtaat caataacgcc gtggaagcgt
    12481 taaaagttat tgccgccccc atgggggagc cgtccggtga tttgctaccg ttcactaatg
    12541 gtgtgcttaa tctgaaaacg ggggaatttt ctccgcactc gccggagcac tggagcacca
    12601 cgcacaatgg tattgagtac acgccaccag tagcagggga aaacatccgc gataatgcgc
    12661 caaatttcca taaatggctt gaacatgctg caggaaaaga cccgcgcaag atgatgcgta
    12721 tatgtgccgc gttatacatg attatggcga accgctacga ctggcagatg ttcattgagg
    12781 ccaccggaga cgggggaagc ggaaagagca catttactca tattgccacc ctgcttgctg
    12841 gcaaacagaa caccgtaagc gcagagatga catcactcga tgatgcagga gggcgcgcgc
    12901 aggttgtcgg gagtcgtctt atcgtccttg ccgatcagcc gaaatatacg ggggaaggca
    12961 cgggcatcaa gaaaatcacg ggaggcgatc ccgttgaaat taacccgaaa tatgagaagc
    13021 gattcacgac gataataagg gcggtggtac tggcaaccaa taacgacccg atgatcttta
    13081 ccgaacgggc cggaggtgtg tcacgccgtc gggtgatttt ccggttcgac aacattgtaa
    13141 gggaggacga aaaagacaag gaattaccgg aaaagatagc ggcagaaatc cccgtaatta
    13201 tccgccgctt gctggctaat tttgctgacc ctgaaaaggc acgggcttta ttactggaac
    13261 agcgtgacgg tgatgaagct ctggcaataa agcagcaaac ggatccggtt gttgagcttt
    13321 gcgcggcgct ggagtttctg gaggaagctc gtgggctaat gatgggcggt ggtggtgaca
    13381 ccgtgaagta cacgaccaga aacagccttt accgtgtcta tatggccttc atggcatata
    13441 caggaaaggg gaaatgtttg agcgtgaatg agttcggaaa ggctatgagg tcagcggcga
    13501 aagtttacgg atatgaatat attacgcgaa aagttaaggg agtcactcag accaacgcaa
    13561 cgactactga tgattgcgat gcgtttttat aaaaaatggc aatggttatc taccttgtct
    13621 acctgactga aataaaatac ttttatttca atgtattaat gcaggtagat aactattttt
    13681 cactgtctac ctgttatcta ccttatctac ccatttttgt agacaggtaa ggagacgggt
    13741 agagatgagg tagacagcta tttggggctg tctacctctc tgaaacccgc gccattactg
    13801 gcctgataac taatcaggta gacaaggtgg tggtgcacaa aaaacttttt aaacgagggg
    13861 gtaaaaataa aaatgcacac atcaggaaaa ctgaacaaac atataaagcc acattaccgc
    13921 gcccttgata tggctgaaca ctggctaagg gtggcgatta aggcaataga ccgcaacgcc
    13981 ggggaaggat acgcgaaagc acatcccgaa ctgataagcg cattcatgac aacggcggct
    14041 gcaaactttg ccacgctgac cgaacgggag attgccgaag cggaggaagt gacaaccatc
    14101 aatattaagt cctgagagca ggcagcatga cggcgcaaat agcagcttac gggcggctgg
    14161 tggacgaccc acaggtaaaa cataccagca agggaacacc catgacactg gcacggatgg
    14221 cggtatcttt gccatgcagc caggcagatg acggaacggc gacgatgtgg ttatctgtgc
    14281 tggcgtttgg cagacaagcc gatgcgctgg caaagcatca caaaggcgaa ctcctgagcg
    14341 tggcgggtaa catgcaggtg agccagtgga ctggacagaa cggtgaaacg cggcagggct
    14401 ggcaggttat cgcagacagc gtaatcagtg cgcgaacggt gcgaccgggc ggcaaaaaag
    14461 gtcaacaggg tcaggctact gacgcactga acagagcaaa acaataggca gatcagcaag
    14521 gaagccatcc accagtggga gataatgagc aatggggaga tgatattccg ttttaaatat
    14581 tgccaataaa aaaggccgga aaaaataaat tttccggcat gctacataaa tcccgaccaa
    14641 agggagtgaa tatattaaca ctaattatcc gcgctgaagt tgtcatccca aaactttata
    14701 caacattgca ctcggttgca tgtattcgca tgacaaatat tggtgatagc atatatccac
    14761 aattattttt aatgaatgca aagaggatgt gtatggttga tttatattcg cctacccagc
    14821 ttgtacaggt ggttaatgct gtagatgtac aaaaacaact aaatgcgttg tttaccagtt
    14881 tgttttttac tcgctcggta atgtttgaat cgcgcgatat tattcttgat acaatcgacg
    14941 atccaaatat cccaattgca gcgttttgtt ctcctatggt gggtagtaaa gtttcacgtg
    15001 acgaagggta cgaatcaaaa acaattcgtc caggctatat gaagccgaaa agcagcattg
    15061 atccaaataa gttagctgtg cgccctgctg gtgtatcacc tgagcaatac aatgcttttg
    15121 gggcgcgtaa tattaaagtt aaacaggcga ttgtaaatca agctaaagct attcgtgcac
    15181 gtattgaatg gcttgctgtt caggcaatca caacggggaa aaatatcatt gagggcgatg
    15241 gtattgaacg ttatgagctg gactggaata taaaaccaca aaatatcatc actcagtctg
    15301 gcggtactga gtggtcaagt aaggataaag aaacttttga tccaaatgat gatattgaga
    15361 gctacgcaga atttagtgag ggcgtcacta atatcatcat tatgggtggt aatgtatgga
    15421 agaaataccg ttcattcaga gcgataaaag aggctttgga tacccgtcgt ggttctaatt
    15481 ccgaactgga aacggccctt aaagaccttg gtgattcggt gagttttaaa gggtatatgg
    15541 gcgatgttgc gattgttgtt tacagcgggc gttataccga cgaggacgga acagaaaaac
    15601 atttccttga tcctgatttg atggtgcttg gcaatacggc tcttcagggg attgtcgcct
    15661 atggcggtat tcaggatccg gagctaatcc gaatggggct gactaaagcc gaacttgcac
    15721 cgaaaaacta tattgtacct ggtgatccgg ctattgaata tgtgcagaca cattcagcac
    15781 cacagccaat accggcccgc atcaatcgtt ttgttaccgt tcgcattggc taagggggag
    15841 caatggctac tcattacact gaactcatgg ctggcactga agcactggtg actacgctgg
    15901 ggatattttc agctaataaa ggggtaattc ctgcatttac gccactgatg caggaagatg
    15961 caacaggtgc actggtggta tgggatggtt cgagcgtagg taaagcggtt tatgtttccg
    16021 ctgtacaaat cgacaccgcg aaaaaaacac gggctcaggt ctataagaca ggtgtcttaa
    16081 atgttgatgc tctgaactgg cctgagtctg taaaagaact gtcagtaaag attgcagcgt
    16141 ttgttggctc aggtatttct gttcagccgc tggctcgtgt gtaaaggggg atacaatgca
    16201 gaatgattac aatgacctta agccaattgc cgaaatgatg tacccgaatc cagctgtaga
    16261 ggaattaaaa gctatcgctg acaaaatgtg tttaagcgag cgccttgttg atatgaatca
    16321 ggtgatggaa attacaaccc tgagtcggcg tacactgcta aaccttgagg ctagtggaga
    16381 gttcccggag cgtgtgcagg ttacggaagg gcgtaaggcc tggtatttaa gtgaagtgat
    16441 cgactggata aataatattc ctcgcgcttc tgaatattgc cgcgtacctg tcccaaaaaa
    16501 gccagatgcg gcgctatgcc tcaagattga gcgtgtacgc cgcaatgcac gggatggtcg
    16561 ctataagctg attggttgat gaaattaggg cccgctctgg ctggcgggtc ctttccggcg
    16621 atctgacagg ctacggggcg tcaggcgcgc gggttttcgc tatttgtgaa aattttcctg
    16681 tttaaggcgt ttccgttctt cttcgccgta acttaatgtt tttattaaaa acacccccta
    16741 aaaagaaagg aaacgacagg tgctaaaaac aggctttttg gcctctgtcg tttcctttct
    16801 ctggttttgt ccgtggaatg aacaatggaa gccaacaaaa agcagctggc tgacattttc
    16861 ggtgcgagta tccgtaccat tcagaactag caggagcagg gaatgcccgt tctgcgaggc
    16921 ggtgacaagg gtaatgaggt gctttatgac tctgccgccg tcataaaatg gtatgccgaa
    16981 agggatgctg aaattgagaa cgaaaagctg cgccgggagg ttgaagaact gcgccaggcc
    17041 agcgaggcag atctccagcc agggactatt gagtacgaac gccatcgact tacgcgtgcg
    17101 caggccgacg cacaggaact gaagaatgcc agcgaaacag gtgaggtaat tgacactgca
    17161 tttgcgactt atgccttgtc gaaattaact ggggaagttg gagcaatcat tgatagtctt
    17221 ccacttgcga tctgtcgaca gtttcctggc atggaaaaac ggtatcagga cttcattaag
    17281 atggaggtca gtaaagcatt tattcgagct taccagatct cagataacat tccagatatg
    17341 gctgaaaggt atatcaagga taatcagcga agttaaaaca aatgaaacca taggtaggaa
    17401 gagtgcttag gtggttcttt gttgcttaaa agtcgccagt aaaactggcg atagatttta
    17461 tttgtcaaaa gcatttgacc ttttaaggta gtggtactat ggatattttt ctgtcgttcc
    17521 caaatgaact tttaaataat ttcgctcttg gatacatctt aactagtgat gcgacttcat
    17581 cgaactcgct ggcatcaaaa agaggaccaa gtattacaga ttttaactca aaagggtgaa
    17641 attttaagtc gctatattga ccagtgctat ttggcctagc gtaggttgaa attctccact
    17701 ctctttcata tgcccattct tctgttttta tgaatattat ctcattattg atatattcaa
    17761 ttgacttatt gtctggcatg aaaagcaact cggccatccc ttcagcagtg tgagttagag
    17821 gcattttatc tgtgtatttc atagctttgg ctattaacca agcgctatcc agaaagtcta
    17881 cacaatcaaa ttcgataact atacctttat atttatcggc atagtgattc cacataggag
    17941 tgattatagg gctctcagat aaacacaaaa tccttctgtt attaagcatt cctcgccaga
    18001 catctttcat ttcttgtatt gccgcaggag cgccaatccc aactggtgga atgtttacca
    18061 tttcttgaaa tctttcaatt aatcctgcgg gtatgcctaa aggaaatgac ttttggaaat
    18121 cacttagcat tgttctaatt ctagggttta aattttgtat gtcttctctg ggggtaatca
    18181 actccgctat taacttgcgg gctaaagctt tgccaatatt aatttcgtta ataccaggca
    18241 taacttctct tggaacatca aaagggtcat taaataggac cggagaactc catctaaggc
    18301 tgcatgaatc aagaacaatc ttcgcggtat tcaacgacat atacttataa aatgttgagc
    18361 gttcatgcat tctgttgggt gagcgcatag attcctcttg ttaaatgagt gatggttttc
    18421 atccatgtat tgcacgttgt attgcattca aggattttcc atttcaattc tatagctaaa
    18481 acgtaatcaa atcattcaca tcctttcata tttgactcat gtagccgaag catgaagtga
    18541 atccgcagat gaccctgcgt cgcctgccgg atgaagatcc gcagaatctg gcggacccgg
    18601 cttaccgccg ccgtcgcatc atcatgcaga acatgcgtga cgaagagctg gccattgctc
    18661 aggtcgaaga gatgcaggca gtttctgccg tgcttaaggg caaatacacc atgaccggtg
    18721 aagccttcga tccggttgag gtggatatgg gccgcagtga ggagaataac atcacgcagt
    18781 ccggcggcac ggagtggagc aagcgtgaca agtccacgta tgacccgacc gacgatatcg
    18841 aagcctacgc gctgaacgcc agcggtgtgg tgaatatcat cgtgttcgat ccgaaaggct
    18901 gggcgctgtt ccgttccttc aaagccgtca aggagaagct ggatacccgt cgtggctcta
    18961 attccgagct ggagacagcg gtgaaagacc tgggcaaagc ggtgtcctat aaggggatgt
    19021 atggcgatgt ggccatcgtc gtgtattccg gacagtacgt ggaaaacggc gtcaaaaaga
    19081 acttcctgcc ggacaacacg atggtgctgg ggaacactca ggcacgcggt ctgcgcacct
    19141 atggctgcat tcaggatgcg gacgcacagc gcgaaggcat taacgcctct gcccgttacc
    19201 cgaaaaactg ggtgaccacc ggcgatccgg cgcgtgagtt caccatgatt cagtcagcac
    19261 cgctgatgct gctggctgac cctgatgagt tcgtgtccgt acaactggcg taatcatggc
    19321 ccttcggggc cattgtttct ctgtggagga gtccatgacg aaagatgaac tgattgcccg
    19381 tctccgctcg ctgggtgaac aactgaaccg tgatgtcagc ctgacgggga cgaaagaaga
    19441 actggcgctc cgtgtggcag agctggaaga ggagcttgat gacacggatg aaactgccgg
    19501 tcaggacacc cctctcagcc gggaaaatgt gctgaccgga catgaaaatg aggtgggatc
    19561 agcgcagccg gataccgtga ttctggatac gtctgaactg gtcacggtcg tggcactggt
    19621 gaagctgcat actgatgcac ttcacgccac gcgggatgaa cctgtggcat ttgtgctgcc
    19681 gggaacggcg tttcgtgtct ctgccggtgt ggcagccgaa atgacagagc gcggcctggc
    19741 cagaatgcaa taacgggagg cgctgtggct gatttcgata acctgttcga tgctgccatt
    19801 gcccgcgccg atgaaacgat acgcgggtac atgggaacgt cagccaccat gacatccggt
    19861 gagcagtcag gtgcggtgat acgtggtgtt tttgatgacc ctgaaaatat cagctatgcc
    19921 ggacagggcg tgcgcgttga aggctccagc ccgtccctgt ttgtccggac tgatgaggtg
    19981 cggcagctgc ggcgtggaga cacgctgacc atcggtgagg aaaatttctg ggtagatcgg
    20041 gtttcgccgg atgatggcgg aagttgtcat ctctggcttg gacggggcgt accgcctgcc
    20101 gttaaccgtc gccgctgaaa gggggatgta tggccataaa aggtcttgag caggccgttg
    20161 aaaacctcag ccgtatcagc aaaacggcgg tgcctggtgc cgccgcaatg gccattaacc
    20221 gcgttgcttc atccgcgata tcgcagtcgg cgtcacaagt tgcccgtgag acaaaggtac
    20281 gccggaaact ggtaaaggaa agggccaggc tgaaaagggc cacggtcaaa aatccgcagg
    20341 ccagaatcaa agttaaccgg ggggatttgc ccgtaatcaa gctgggtaat gcgcgggttg
    20401 tcctttcgcg ccgcaggcgt cgtaaaaagg ggcagcgttc atccctgaaa ggtggcggca
    20461 gcgtgcttgt ggtgggtaac cgtcgtattc ccggcgcgtt tattcagcaa ctgaaaaatg
    20521 gccggtggca tgtcatgcag cgtgtggccg ggaaaaaccg ttaccccatt gatgtggtga
    20581 aaatcccgat ggcggtgccg ctgaccacgg cgtttaaaca aaatattgag cggatacggc
    20641 gtgaacgtct tccgaaagag ctgggctatg cgctgcagca tcaactgagg atggtaataa
    20701 agcgatgaaa catactgaac tccgtgcagc cgtactggat gcactggaga agcatgacac
    20761 cggggcgacg ttttttgatg gtcgccccgc tgtttttgat gaggcggatt ttccggcagt
    20821 tgccgtttat ctcaccggcg ctgaatacac gggcgaagag ctggacagcg atacctggca
    20881 ggcggagctg catatcgaag ttttcctgcc tgctcaggtg ccggattcag agctggatgc
    20941 gtggatggag tcccggattt atccggtgat gagcgatatc ccggcactgt cagatttgat
    21001 caccagtatg gtggccagcg gctatgacta ccggcgcgac gatgatgcgg gcctgtggag
    21061 ttcagccgat ctgacttatg tcattaccta tgaaatgtga ggacgctatg cctgtaccaa
    21121 atcctacaat gccggtgaaa ggtgccggga ccaccctgtg ggtttataag gggagcggtg
    21181 acccttatgc gaatccgctt tcagacgttg actggtcgcg tctggcaaaa gttaaagacc
    21241 tgacgcccgg cgaactgacc gctgagtcct atgacgacag ctatctcgat gatgaagatg
    21301 cagactggac tgcgaccggg caggggcaga aatctgccgg agataccagc ttcacgctgg
    21361 cgtggatgcc cggagagcag gggcagcagg cgctgctggc gtggtttaat gaaggcgata
    21421 cccgtgccta taaaatccgc ttcccgaacg gcacggtcga tgtgttccgt ggctgggtca
    21481 gcagtatcgg taaggcggtg acggcgaagg aagtgatcac ccgcacggtg aaagtcacca
    21541 atgtgggacg tccgtcgatg gcagaagatc gcagcacggt aacagcggca accggcatga
    21601 ccgtgacgcc tgccagcacc tcggtggtga aagggcagag caccacgctg accgtggcct
    21661 tccagccgga gggcgtaacc gacaagagct ttcgtgcggt gtctgcggat aaaacaaaag
    21721 ccaccgtgtc ggtcagtggt atgaccatca ccgtgaacgg cgttgctgca ggcaaggtca
    21781 acattccggt tgtatccggt aatggtgagt ttgctgcggt tgcagaaatt accgtcaccg
    21841 ccagttaatc cggagagtca gcgatgttcc tgaaaaccga atcatttgaa cataacggtg
    21901 tgaccgtcac gctttctgaa ctgtcagccc tgcagcgcat tgagcatctc gccctgatga
    21961 aacggcaggc agaacaggcg gagtcagaca gcaaccggaa gtttactgtg gaagacgcca
    22021 tcagaaccgg cgcgtttctg gtggcgatgt ccctgtggca taaccatccg cagaagacgc
    22081 agatgccgtc catgaatgaa gccgttaaac agattgagca ggaagtgctt accacctggc
    22141 ccacggaggc aatttctcat gctgaaaacg tggtgtaccg gctgtctggt atgtatgagt
    22201 ttgtggtgaa tgatgcccct gaacaggcag aggacgccgg gcctgcagag cctgtttctg
    22261 cgggaaagtg ttcgacggtg agctgagttt tgccctgaaa ctggcgcgcg agatggggcg
    22321 acccgactgg cgtgccatgc ttgccgggat gtcatccacg gagtatgccg actggcaccg
    22381 cttttacagt acccattatt ttcatgatgt tctgctggat atgcactttt ccgggctgac
    22441 gtacaccgtg ctcagcctgt ttttcagcga tccggatatg catccgctgg atttcagtct
    22501 gctgaaccgg cgcgaggctg acgaagagcc tgaagatgat gtgctgatgc agaaagcggc
    22561 agggcttgcc ggaggcgttc gttttggccc ggacgggaat gaagttatcc ccgcttcccc
    22621 ggatgtggcg gacat
//
LOCUS       DBBGLQ010000068        22368 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.068, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000068 DBBGLQ010000000
VERSION     DBBGLQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.068"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..811)
                     /locus_tag="ACIYSB_003553"
     CDS             complement(<1..811)
                     /locus_tag="ACIYSB_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGC5430520.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVR"
     gene            complement(834..1817)
                     /gene="yphF"
                     /locus_tag="ACIYSB_003554"
     CDS             complement(834..1817)
                     /gene="yphF"
                     /locus_tag="ACIYSB_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGC5430521.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(1914..5195)
                     /gene="yphG"
                     /locus_tag="ACIYSB_003555"
     CDS             complement(1914..5195)
                     /gene="yphG"
                     /locus_tag="ACIYSB_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGC5430522.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQHSLRTCPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            5313..6506
                     /gene="yphH"
                     /locus_tag="ACIYSB_003556"
     CDS             5313..6506
                     /gene="yphH"
                     /locus_tag="ACIYSB_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGC5430523.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESHTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDHTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(6566..6664)
                     /locus_tag="ACIYSB_003557"
                     /pseudo
     CDS             complement(6566..6664)
                     /locus_tag="ACIYSB_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6704..7957)
                     /gene="glyA"
                     /locus_tag="ACIYSB_003558"
     CDS             complement(6704..7957)
                     /gene="glyA"
                     /locus_tag="ACIYSB_003558"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGC5430524.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            8285..9475
                     /gene="hmpA"
                     /locus_tag="ACIYSB_003559"
     CDS             8285..9475
                     /gene="hmpA"
                     /locus_tag="ACIYSB_003559"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGC5430525.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANFGDVVKLVAPAGDFFMAVSDDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(9520..9858)
                     /gene="glnB"
                     /locus_tag="ACIYSB_003560"
     CDS             complement(9520..9858)
                     /gene="glnB"
                     /locus_tag="ACIYSB_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGC5430526.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDICVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(9919..11253)
                     /gene="glrR"
                     /locus_tag="ACIYSB_003561"
     CDS             complement(9919..11253)
                     /gene="glrR"
                     /locus_tag="ACIYSB_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGC5430527.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(11243..11956)
                     /gene="qseG"
                     /locus_tag="ACIYSB_003562"
     CDS             complement(11243..11956)
                     /gene="qseG"
                     /locus_tag="ACIYSB_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGC5430528.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(12121..13548)
                     /gene="qseE"
                     /locus_tag="ACIYSB_003563"
     CDS             complement(12121..13548)
                     /gene="qseE"
                     /locus_tag="ACIYSB_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGC5430529.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLHNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(14106..17993)
                     /gene="purL"
                     /locus_tag="ACIYSB_003564"
                     /gene_synonym="purI"
     CDS             complement(14106..17993)
                     /gene="purL"
                     /locus_tag="ACIYSB_003564"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGC5430530.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELREILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            18251..19807
                     /gene="mltF"
                     /locus_tag="ACIYSB_003565"
     CDS             18251..19807
                     /gene="mltF"
                     /locus_tag="ACIYSB_003565"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGC5430531.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(19804..20307)
                     /gene="tadA"
                     /locus_tag="ACIYSB_003566"
     CDS             complement(19804..20307)
                     /gene="tadA"
                     /locus_tag="ACIYSB_003566"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGC5430532.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(20365..21000)
                     /gene="yfhb"
                     /locus_tag="ACIYSB_003567"
     CDS             complement(20365..21000)
                     /gene="yfhb"
                     /locus_tag="ACIYSB_003567"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGC5430533.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            21209..22057
                     /gene="yfhH"
                     /locus_tag="ACIYSB_003568"
     CDS             21209..22057
                     /gene="yfhH"
                     /locus_tag="ACIYSB_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGC5430534.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
BASE COUNT         5345 a         6030 c         5683 g         5310 t
ORIGIN      
        1 cacggacttc cagtacggcc tgatccacaa tttcctgagg ggctaccggc gcaatatcaa
       61 cgtgatcacg ccctagcatt agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca
      121 catcgcccgc cacctgacca tcgcgcataa cggtggcaca ggaggcaatg cggcgaattt
      181 cttccatccg gtggctgaca taaatcaccg ccacgcccag tgccgacatc tttttcaccg
      241 cgctgatcac cagttcaact tccgcactcg caagcgaact ggtaggttca tcaagaatga
      301 ccacgcgcgg ctcgcccttc atcacccgcg caatttccac cagctgcttt tgcgccgggc
      361 ttagcgttga aacaagttgt tcaggactaa cgtcaacgcc cagcgcctgt aagcaacgtt
      421 gggcatcctg cgccatttgc aggtaatcaa tcatgccgtt gcggcggggc cactgaccga
      481 ggcagaggtt ttccgccacc gtcagccctt ccaccagact taattcctga taaaccgcac
      541 gaacccccag ttcagcggca cggcgagtca acgtagcttc gtcaccttcc agtcgcgtct
      601 cgccaatcca gatatcaccg ctatccgggc gttcgctgcc ggtaagcatt cgaatgagag
      661 tcgatttgcc cgcgccgttt ttacctaaca gcgcacgaac ttcgccttta ttgagcgtga
      721 agttaacgtt atccagcgca acgacgccgg ggtaacgctt atttcctgcc accacttttg
      781 ctaccgggac tgcctctgtt gccgtgaaca tagggacctc tgcgaatcag cgattagggc
      841 agaccatcaa cgtgcgttgc cagccactgt ttaccatctt ccgttttggt atagagatcg
      901 attggcacct gaatcacttt ttcaccgtcg gcttgtttat tgataacctt caatgtttgc
      961 gcgaaaacag cattgcccat tttcttaccg gaaatatcca ctaccgcttt cagcacctga
     1021 ttgttttcca gctcctgagc aatttcggtt gtcatatccg aaccgaaaac agcaattttt
     1081 ccggcctgat tttgattacg taccgctttt accgcgccga gtgtcgcacc gcccgactcc
     1141 cccataatgg cgttgagatc cggcgtggag ataatcagtt tttcaccaac ggaaatcgct
     1201 ttatctaaaa cagtcccttc ctgattagcg acaatttgcg cgccgggaac gcgggatttt
     1261 aatacttctt caaatccttt acgtcgctgc acacaaactt caaaggcttc gcaattgatg
     1321 acggcaattt tcggctggtc aattttattg gcaataaaat aatcggcggc agcgttaccc
     1381 agttttttac caaattccag tggatcgccg accagatacg ccgagacata tttatcgaca
     1441 cccttttgat taatacaggt gttgtagcaa atcaccggaa tacccgcttc actggcgcga
     1501 cgaacggtac ggctactgcc attttcagac actgccgata aaataatggc atcgacatta
     1561 cgcgccacga gggtatcaac aaaggtactt tctttcgaaa tatcaccctg ggcgttagtt
     1621 tcaattaact gtacctgtac tgaagaatct tttgccgcat cctgaacgcc ctgacgcact
     1681 ccagcgtaat atccctgggt atcaaggtat attgcaccaa tggtcatttc tttatccgca
     1741 gccctggcaa atagtgcgct acctaatagc gtagccatta ataatagatt acgggttgtt
     1801 ctcatttttt taggcatagt gcctcctgta gggtttttat taacaacggc ttattctaat
     1861 tattttgtga tgagcggcag cgcatgccgc tcaaatatta cagttaatag ggtttagtga
     1921 acgtagttaa aaataaaagg catcacggtg gtgaataatg ccgctttcgg ccaggccgga
     1981 ttaatcgccg tcaattcagc gcgagcagat tcatattgcg caacctcccc gagtcccgcg
     2041 gacgccagca tcgccaccat caggcatttt tctttatgct gctgttgtaa atcgccatac
     2101 agcgacaaca ggtcaggttg tgaaacagca aagaaatccg cctcaatact ggttttcgcc
     2161 atctcttgcg cccactgttt catttcacta aacagttgct gtgcggtttg ctgttcaccc
     2221 agcagtcgca gcgccattcc ttgccagaag agataatcaa ccggctgatc gttgtaataa
     2281 ctgtggatgt taatggtgcg atcgccggtc gccgccagac gtaaacaacg catcgcttca
     2341 gtttcatcgc cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc gttatcagtt
     2401 tgccccggta aacggccttc gcttaaattc tccggataat gcagcgcggc atgaagcagt
     2461 tcgctggcct gctgcggctg tctggcatca agatgctgcc aggcgcgtaa taactggttg
     2521 aggataaact gactggtgac cttcccttcc ccgccttccc acgggtggaa tttacgcgtg
     2581 gcgagaatgt ccgccgcttt gtctgcctga cccgtgagat gccacaaatt gagcagttct
     2641 gcggtcatgt cgtcgcgttt cagcgcaatt tcctgattat tttccagacg cgccagtcgt
     2701 ttctccggtg tggctccact taacttatca agcaaatccc gttcgaaaag cagacgtgca
     2761 tcctgcggcg caagctgata agcattatca agataacgcg cggccagctc ataatcgtgt
     2821 tgcttattcc acgcatggat cgctaacccg cgccagccgt cggcaaactc cggcgacatc
     2881 tctacgcaac gttgccataa ggcaatggct ttgttgtagc tacgtttgtt gtagtagaag
     2941 caggccagta aatggcgagc aaaccagcac tcttcaatac tctccagcgc cgccacttct
     3001 tccaacgtat tcgggaaacg gacaaactgc gggaagacat caatggcttt tgcgaccagt
     3061 tcgccacgtt cggctttcgg cagcaagctg gcttgcaggt aaagcggcag cgtgcgctgg
     3121 cagtccagtg cgttcagcat ctctgccgcc agggtgggca ttccccagtt aatcagttgc
     3181 ccggcggtca tcagggcgtt aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgaga
     3241 gctgattcgc tacgaccatc gaaccagttc agccaccaca gagtggcgtt cagcggataa
     3301 tcgcgcagca gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact taacaccagc
     3361 agcagattat gcaggcaaag cacttcctga ttggttgggc aggtgcgaag actgtgttgg
     3421 caaaaatcca gacccgcgtc gaagtttcca ttacgcgccg ccagtcgtgc cagaccataa
     3481 tagccgcctg ctttgctgtt gccgctccag accgcacgcc agaaatcctc ttcggcttgt
     3541 tgatattgtc cctgacgttc gtaagcactg gcgcgaatca aactcgcctg tccgcactgc
     3601 ggatttttgt tcagcgcatg tgcgcgtttc agcgcctgag tggcatacgc caccgcttgt
     3661 gggaaatctg cgcggttata ttccagcatc gccagcgcca ggttacagcg gtaatccagc
     3721 ggatccagcg ccacaccgcg caggtagtaa tcgaacggtg aacggctggc gtgatgatat
     3781 tgctccagat gctgaccgat aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc
     3841 agtggcgctt tggcgacgtc cggcaacggc aactcttgcg gctgatgttc ctgataactc
     3901 agtacaatat tgccgtcggc atcggagagc tcaatggtca gcctttcagg attgataccg
     3961 tgcaacacgc cctggatggc ggtcgctggt gtcaaggcca cggcatcatc aagtaacgcg
     4021 ttgcatttgc cgatttcgcg gatcgccagg cggtatccgt tcaacggaga gatggcatac
     4081 agcccccact caatcccccg ctcactacgc tggagtttta tcaccgcatc gcgggaggca
     4141 ttttgcacca tgcccagaga atgataaggc aggaaatact gctcgaaacg cttctcttcg
     4201 taagcatcaa gccaggtaaa atcaggctgg ttatcggcaa aaataccggt catcagttcg
     4261 atatacgggc cgttattgtc ggtcaggctc ttgtcccacg cctggccaaa ttcactgtgt
     4321 ccccaactcc actgtttttt acctggcgca atatggtggt tggcaacgtg cagcaaaccg
     4381 ccatcttcat cgtgacacca cgcgccaaca aaatcgtact gtgatttttc agccatatat
     4441 gaggttggaa cgggcacatt tttatagcga gaaatgtcca ctccagcgga gtagtccact
     4501 ttgtagtaag tgccggtggc gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac
     4561 accgccgtta catccggcgg gaaaacgctc tgatgccctt cccccccttt cactgccggg
     4621 ttggcccacc acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt
     4681 tccagcgccg cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa accatgcatc
     4741 ggctccgttt cgccgaccca caccgtctgt gcgccgtctt catgggcttc gagggtgaaa
     4801 tcaacgggca taaaggtggt cgggcgatgg tgttgcggcc agttaaactc aatcccaccg
     4861 gagatccacg gtcccagcag ccccaccagc gcaggtttaa tgacttcatt gtgataaaca
     4921 aaatcgcgtt gtttcacttt atcccatgcg cgatgcacac gaccgcccag ttccggcagg
     4981 atcatcactt tgatgtagtc gttttccagc cacaccgcct gccaggattt cagggttttc
     5041 tgctcgctca gcgtatcggt cacgccatag ggataaaccg cgccggacga tccctgataa
     5101 acgcgatttt ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc
     5161 tcaacgcgct cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat
     5221 actgaattaa aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa
     5281 tcacgcaatt aactaaacaa ggtttagtga agatgagagc ctgcattaac aatcaacaga
     5341 tccgccacca taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata
     5401 aatcaacgct ggcccgactg gcgcaaattt cgattccggc agtcagtaat attttgcagg
     5461 aactggaaag cgaaaaacgg gtggtgaata tcgacgatga aagccatacg cgcgggcata
     5521 gtagcggtac atggctgatt gcgccggaag gtgactggac gctgtgcctg aacgtgacgc
     5581 ccaccagtat tgagtgtcag gttgctaatg cttgtttaag tccgaaaggt gaatttgagt
     5641 atttacagat tgatgcaccg acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc
     5701 atcgccaccg taaattgtgg ccggaccata ccatcaacct ggcgttggca atccacggtc
     5761 aggttgatcc tgtgaccggc gtgtcgcaaa ccatgccgca agcgccgtgg acaacgccgg
     5821 tggaggtgaa atatctgctg gaagagaagc tcggcattcg ggtgatggtc gataatgact
     5881 gcgtgatgct ggcgctggcg gagaaatggc aaaataattc gcaggaacgg gatttctgcg
     5941 tgatcaacgt tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg
     6001 gcagtttgta tggtagcgga cagattggtc acaccatcgt taatccggat ggcgtcgtct
     6061 gcgactgtgg acgttatggc tgcctggaaa ccgtcgcctc gttaagcgca ttaaaaaaac
     6121 aggcgcgggt atggctaaaa tcacaaccgg ttagtactca acttgatcct gaaaaactga
     6181 ctacagcgca gttaatcgct gcctggcaaa gtggagaacc gtggatcacc agctgggttg
     6241 atcgctctgc caatgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta
     6301 atcagatttg gttgtacggt cgcagttgtg cctttggtga gaactggctt aatactatta
     6361 ttcgccagac aggatttaac ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc
     6421 aaattggctt tgggcaatta agccgcgcac aacaggtgct gggaattggc tatttgtatg
     6481 ttgaggcgca gttacgacag atttgatggc gcgataacgt agaaaggctt cccgaaggaa
     6541 gccttgatga tcataaacga aaaattgcct gatgcgctac gcttatcagg cctacacgga
     6601 gattgcaata tattgaattt gcaaagtttt gtaggccgga taaggcgttc acgccgcatc
     6661 cggcatgaac aacgagcaca ttgacagcaa atcaccgttt cgcttatgcg taaaccgggt
     6721 aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct tcatcattga
     6781 tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttcggct tctttaaagc
     6841 cgcgacgggt aatcgccgga gtacctacac gaataccgga ggtcacaaac gggctcttcg
     6901 gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga gcggcgtctg
     6961 cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc
     7021 cgccggaaac cactttgtag ccgcgctcga ggaacacttc taccatcgct ttagcgtttt
     7081 tagcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc agagcaaccg
     7141 ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa acggcagagt
     7201 tcagtttttt gtacagctct tcgctaccac ctttcgccag gatcaggccg ccgcgcggac
     7261 ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc gggttcgggt
     7321 agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacagg taagcaccga
     7381 tgctgtcagc gatttcacgc attttcgccc agtccaccac gccggaatat gcagagaaac
     7441 caccgataat cattttcggc ttgtgttctt tggcttgttt ttccagatcg gcgtagtcga
     7501 tatgaccggt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa
     7561 ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga acggtatcac
     7621 ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag tgcggctgga
     7681 cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt tgttcaacga
     7741 tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca gcatatttgt
     7801 tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga
     7861 tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc cacagttcgg
     7921 catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga ctcagctaac
     7981 aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag tataaccgaa
     8041 tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc ttcaggctac
     8101 gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc aggtttgtga
     8161 tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca tttacattgc
     8221 agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa aaaaggaaga
     8281 ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc accatccctt tactggtgga
     8341 aacggggcca aagttaaccg cccatttcta cgaccgtatg tttactcata acccagaact
     8401 caaagaaatt tttaacatga gtaaccagcg taatggcgat caacgtgaag ccctgtttaa
     8461 cgctattgcc gcctacgcca gtaatattga aaacctgcct gcgctgctgc cagcggtaga
     8521 aaaaatcgcg cagaagcaca ccagcttcca gatcaaaccg gaacagtaca acatcgtcgg
     8581 tgaacacctg ttggcaacgc tggacgaaat gttcagcccg gggcaggaag tgctggacgc
     8641 gtggggtaaa gcctatggtg tactggctaa tgtatttatc aatcgcgagg cggaaatcta
     8701 taacgaaaac gccagcaaag ccggtggttg ggaaggtact cgcgatttcc gcattgtggc
     8761 taaaacaccg cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg acggtggcgc
     8821 agtggcagaa taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc
     8881 gcatcaggaa attcgtcagt actctttgac tcgcaaaccg gatggcaaag gctatcgtat
     8941 tgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc acgccaattt
     9001 tggcgatgtc gtgaaactgg tcgctccggc aggtgatttc tttatggctg tctcagatga
     9061 cacaccagtg acgttaatct ctgccggtgt tggtcaaacg ccaatgctgg caatgctcga
     9121 cacgctggca aaagcaggcc acacagcaca agtgaactgg ttccatgcgg cagaaaatgg
     9181 cgatgttcac gcctttgccg atgaagttaa ggaactgggg cagtcactgc cgcgctttac
     9241 cgcgcacacc tggtatcgtc agccgagcga agccgatcgc gctaaaggtc agtttgatag
     9301 cgaaggtctg atggatttga gcaaactgga aggtgcgttc agcgatccga caatgcagtt
     9361 ctatctctgc ggcccggttg gcttcatgca gtttaccgcg aaacagttag tggatctggg
     9421 cgtgaagcag gaaaacattc attacgaatg ctttggcccg cataaggtgc tgtaatttga
     9481 tgttgccgga tggaaacatc cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc
     9541 ttcaccggta cggatgcgaa tgacccgtgc cacgtcaaag acgaagattt taccgtcacc
     9601 gattttgccg gtttgcgccg tgcgaataat ggtatcgaca cagatatcga caatgtcgtc
     9661 cggtacgaca atctcaattt tcactttcgg cagaaaatcc accatatact ccgcgccgcg
     9721 gtacagctcg gtatggcctt tctggcgacc aaagcctttc acttcggtca ccgtcatgcc
     9781 ggtaataccg acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg gttttataat
     9841 cgcatcaatc tttttcatgc tattccttga aaaggtcgcc tgtcttttga tctgctaaac
     9901 gtaacacata acgccaattc attccttgaa atcgtttgca tccagctcgt gtcgggaaag
     9961 cagtttataa aattctgtcc ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc
    10021 tttggtaatt tgcagcagct tacgcaaata gttgagttca aactgattac gtgcctcaac
    10081 aaaggttggc agcgccgtgt tttcaccctc cagcgcctgc tctactaatg catcgctaat
    10141 aaccggagat gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt
    10201 acccggccag ctagcggtca tcaggcgttt catcgcatca gtggagaacg cgcggacaaa
    10261 cggtttatgt cgctctgccg cctggcgcaa caggtgattt gccagaagcg gaatgtcttc
    10321 tgtgcgctct gccagagccg gaattttcag gctgacaacg ttgaggcggt aatagagatc
    10381 ttcgcggaat tccccgcgcg ccatcgcttt tggcagatca cggtgagtgg cagaaataat
    10441 ccgcacattg atatcaatat cgcggttact gcccagcggg cgcactttac gctcctgcaa
    10501 cacgcgcagc agtttgacct gtaacggcgc aggcatatcg ccaatttcat cgagaaatag
    10561 cgttccgcct tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc
    10621 gccacgcgca tgaccaaaca gctccgactc cagcaattgc tcgggcaatg cgccacagtt
    10681 aatagcaata aatggtttgc tgttgcgcgg gctggcgttg tggatagcct gggcgaaaat
    10741 ctctttcccg gtgccgctct gaccgttaat caaaacgctg acgtctgatt gcgccaccag
    10801 ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg gtgacaattg cctcgcgcca
    10861 gcgttcatcg gtggctggcg cggattgctc cagcgcatcg tcaattgcct gatatagcgc
    10921 gtctttgtcg acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc
    10981 atcggggata gaaccatgcg cagtaagaat aattactggc attcccggct gcactttctg
    11041 gatttcagca aatagctgca taccgtccat ttcatccatc cgcaggtcgc tgataactaa
    11101 atctactttt tcgcgattca gtacccgtaa tccttcagcg ccactttccg ccgtgaccac
    11161 actgtagcct tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg gatcgtcatc
    11221 gaccaataat aaatgcgcag gtttatggct catcaggagt gacctcatgg gtggatggcg
    11281 cgggcttttc actctcgtgc ggcgtatccg gcgagaaatt tccagccggt ttgcgggtcg
    11341 agagctggcg ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt tccagttgct
    11401 gctgtagaac gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg gactgctgga
    11461 gtttactgta acgttggcgc tcttccgcca gttgcagttg cagcgcctga ccatcgcgcc
    11521 agagttgata cagtgggcga acctgtgccg ggatctcggt acttaacgct tcaatacgcg
    11581 cgaccagctg gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt
    11641 taaaggtatt ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag
    11701 gcattaaacg atcagcacaa tccatcgccc gcagccagta aagcggattg gtttcggttg
    11761 atttaccttg cagcgcccag atgtcgctac attcagtaga aagatagtca gccagttgat
    11821 aaaccggaat tttttcttct gctggcgtat caatggctgg cttattgtga ttctgcacgc
    11881 aacccagcaa tgccagacat ggcagccctg ccagccacag ccgtcggggc aataatcgtt
    11941 gaaaaatgtg tcgcatattc accagactta aagcctatcc cagtgggcgt aattgttgca
    12001 gacagtctgg acatggacag cgcggagaaa ccggagcgta catatcgtac gtgaagattt
    12061 cgagcactgc ccggggccga actgacaaat aaaatagcct gataggatag gctgttagca
    12121 ttatttcgtg tttttcgacg acggtaattc aatgcggaaa caaacgtctt gcccgctctc
    12181 gtcgaccaga tacagttccc cttgcatacg gcgaatacaa tccctggcaa tgcttaatcc
    12241 cagaccgctg cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa agggttcgaa
    12301 gatcatggcg cgttcctctt gcggaatggg cgtgcctgta ttgatgacat caatataaac
    12361 ccgcgcacca tgtaaactgc tgcgaaggca aatgttaccg gattcagccc cgtagtgcac
    12421 cgcattggag taaagattat ccagtacgct catcagcagc attggctccg ccaggcaagc
    12481 tgttgctttg agatcgacgt cggtatgcat cattttagcc cgtgcgggca ggctatgagc
    12541 agaaaccact gtctccacca gcggtgctaa ctcaacattc tccagttcca ccgcactgtc
    12601 cgcctgttta cggttgtaat caagcagttg ttcgatcagt ttttgcaaat tgcggctgct
    12661 gctatcaaga atgctcacca cctctttttg ctctggcgta agcggcccga caacctggtc
    12721 agccagtaat tcagtgccct cgcgcatact cgccagtggc gtttttaatt catgagataa
    12781 atgtcttaaa aattgatggc gttgggattc cagccatgac aggcgctcac ttaaccaaag
    12841 aatacgttgc ccaaccgagc gtaactcgct cggtccactg aacgagacgc tattgcccag
    12901 agaacgccct tcccccagac ggttgatcat gcgctcgata tttttcaccg gcccgataat
    12961 catccgcgtg aaaagcagta ccattaccag actcaccaga aatagcacca gcgattgcca
    13021 accaaaatat tgcccacgtt cggcgatttc acgctgaagc tgctgcccac gagagaacac
    13081 cactgtgcgc gtggcctgta ccatttcggt attggcactg gcaaaggctt caagacgtgc
    13141 ggcggcggca gcatcgggac cgctgttgtt acactgaagt tgagccagat tgtgcaaatc
    13201 ctgacgtaat gcctggtaga gtttatcgtc cggcagcacg cctgcgtggg catcgagcat
    13261 ttcgctgtaa cgcttgcgct ggctttgata aaccttcgcc agggttgggt cgtccagcac
    13321 gcaatactga cggtaactac gctccatctc cagcgccgcg ttggtcatcg cttcactgcg
    13381 ccgggcatca ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa gcgcattcag
    13441 gctttgccag gcttgccatg ccagcaccaa caggggcagc agaatcagca aaaatgccag
    13501 cattaccagt tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg tgttactctc
    13561 gtcagacgcg aatagcctga tgctaaccga ggggaagttc agatacaaca aagccgggaa
    13621 ttacccggct ttgttatgga ataaggcggt gcctaactcg acgtttcgcc cgatggttga
    13681 tatagctacg ctgatatcag aagttggacg gcaggcacct tgttgtgcgt cattcgtatt
    13741 ttatgtagca cgtcccgaag gggctgacat aagtcggtga atgagccact ggttactatt
    13801 atgcagtaac tgtgccaata aagaaaatag tttggtaacg tactgattat acgttgcttt
    13861 gaggggttta tgtctcctcc gctgtttgaa tgtaaatcag ccactgtgtc gctaaaaaga
    13921 gacaacttaa gataaactta ttagataata ttaaaatcaa tgagttaagt gtcgccagaa
    13981 agcgacacgg caaaccaccc attgtcgtga tttacagaca caaaaaagct cccggagttg
    14041 ggagcttatg atagtggttg gtgcttaatg ccgcatccgg gctgcaaaac caatgggctg
    14101 acgacttacc ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg
    14161 ccccagtttt ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc
    14221 atcataatgg tgactcgacc actttcagtc gtgactgccg taataccgtt cggtgaaccg
    14281 ttcgggttag ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc
    14341 accagccctt tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct
    14401 tcaccatgag agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac
    14461 gggctttggg ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtattgcgc
    14521 acaaaacgtg gccacaactc actacctggg atcagttcac gcagattaga catcatctgg
    14581 caaccgttac atacccccag cgccagcgtt tgcggacggt ggaagaaggt tgcaaactca
    14641 tcgcgtacac ggtcattgaa caggattgac ttcgcccaac cttcaccggc acccagcaca
    14701 tcaccgtagg agaaaccacc gcacgcgacc agggcgtgga aatcttccag gcccgtgcgt
    14761 ccggtcagca ggtcactcat atgcacgtcg atagcatcaa agcctgcacg gtggaaagct
    14821 gccgccattt caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt
    14881 gcgccagtgg caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca
    14941 ttcaggcccg gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg
    15001 ttgtcacgca ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtggtg
    15061 cggctttcgc tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc
    15121 gcctgcccta cataatggac acaatcagca agcccatgct gtgccagtac ggactcgacc
    15181 gcttcacggt cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc
    15241 gccaggcgat cgtcacccag agtggcgata tccgcgtcaa tgccacaatg accagcaaag
    15301 gccatttccg ccagcgttac cagcaggccg ccatcagagc ggtcgtgata cgccagcagc
    15361 ttacgctgtg caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg cgcgacatcg
    15421 cgtacatctg ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc
    15481 agcgcgttat tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtagaaagc
    15541 tgcggcgtga tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac
    15601 agcggcgacg tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacatg
    15661 gagtctttac ccaccgggat cgtcaggccc agcgccggac aaagctcttc gcccacggct
    15721 ttaacggctt catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg
    15781 gcggaaagtt tgatgcgttt gatatcgcca atttgtgttg cggcgatgtt ggttaacgct
    15841 tcaccgaccg ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg
    15901 ccaatcgcca tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta
    15961 gcgaccggca cctgccacgg ccccaccatc tgatcgcgcg ctaccatgcc ggttacgctg
    16021 cggtcgccaa tggtcaccag gaaggttttt tccgccacag tcggcagatg cagcacacgt
    16081 ttcaccgcgt ctgcaatggt gatcccttca cgggccagcg cgtcgccttt cgctttcagc
    16141 gtttgtacat cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg
    16201 atcggctgat tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca
    16261 ccaatcaccg cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga
    16321 tcggcagcaa ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg
    16381 ctcatgcccg gttcgtcgct tagaatctcg cgcagttcaa atttaccgcc gcgcccgccg
    16441 tcgctcacca gttccggcat ggcgttagaa agaccgccag cgccaacgtc gtggataaac
    16501 aggattgggt tggcatcacc aagctgccag caacggtcga tcacttcctg gcagcgacgc
    16561 tccatctccg ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca
    16621 gacgccatag aagacgctgc accaccgcca agaccgatgt tcattgccgg gccgccgaga
    16681 acgaccagct tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg
    16741 ccgatcccgc ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccgttgtgg
    16801 ctgttcactt tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg
    16861 ttgttaaacg ccgcgccgcc cagcgggcct tcggtcatga tgtccagcgc ggtgacaatg
    16921 cgctcaggct taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg
    16981 gaaacggaga aaccaaccag acccgctttc ggctttgcgc cgcgcccggt ggcaccttca
    17041 tcgcggattt caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg
    17101 tggttgtgag tttcgacttt catcagaata tgcgccggtt cctgatggaa atcgtagcgg
    17161 cccgtttcgt ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta
    17221 tctttataag cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcttg
    17281 aacagcgatt tcggctgctg ttcaccatcg ataacccagt cggcgttaaa aattttgtgg
    17341 cggcagtgct cggagttcgc ctgggcaaac atatacagtt cgatgtcgtt cgggttacga
    17401 ccaagctttg tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca
    17461 agacgcaggt tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgctggta
    17521 accggagtcg gttgatggtg ggcaaacaac tgctctgcat catctaaagc aaaaaagacc
    17581 gtttccatca tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc
    17641 gtaccggctt ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg
    17701 cagttatggg caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg
    17761 gtcaccagca ggagtttgcc ttgcggggcg tggctggcga gtgccgggcc atatttcagc
    17821 aggcgttcaa gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg
    17881 acatactcgg cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt
    17941 ttgttgattc ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc
    18001 tcgtcttcta agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct
    18061 gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt
    18121 gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt
    18181 tatgagtaaa cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag
    18241 aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct
    18301 gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc
    18361 cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa
    18421 cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta
    18481 cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct
    18541 tgataatggt aacgccgacc tgctggcggc aggacttgtc tataacagtg agcgggtaaa
    18601 aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg
    18661 tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg
    18721 tcatgtggtg gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg
    18781 gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact
    18841 cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct
    18901 cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg
    18961 cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac
    19021 gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac
    19081 gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa
    19141 atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg
    19201 ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac
    19261 cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt
    19321 gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga acgagcggat
    19381 ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct
    19441 gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt
    19501 acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg gacatgaagc
    19561 ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga
    19621 gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc
    19681 gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc
    19741 aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca
    19801 aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg
    19861 aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac
    19921 tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt
    19981 cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca
    20041 cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat
    20101 caccagacca ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc
    20161 aatcgggcgg ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac
    20221 cggcacttcc cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccagta
    20281 ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat
    20341 tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac
    20401 tcgccaacga tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc
    20461 actgtacagc cgcagcggag tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc
    20521 cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag
    20581 attaacccgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga
    20641 gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg
    20701 aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt
    20761 ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca
    20821 gcgtgccgca cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac
    20881 aagtaacgca ttcagcggtt ggcgacgtag taaatagcgc agaaaactgc cgaacatatc
    20941 ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa
    21001 accatactcc tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga
    21061 tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc
    21121 atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg
    21181 tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta
    21241 ccaggggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac
    21301 ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt
    21361 cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag
    21421 tgaagcgctg gcaagccagc cggaatcacc ctccgtgccc attcataacc aaatccgcgg
    21481 tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta
    21541 cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc
    21601 tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc
    21661 ctggaagctg atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc atgcgctact
    21721 cgcaacagta caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt
    21781 acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc
    21841 gattaccggc tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat
    21901 tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt
    21961 aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat
    22021 tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc
    22081 cgcccggttt gtgttgtttt gagagtttcc ttatggcgtt gttaatcact aaaaaatgca
    22141 tcaattgtga tatgtgtgaa attaaataaa aatacattta ttttcatggt tatgcagtaa
    22201 tgtgttattg aatgtgttgt tggatttatg atgcaaaaaa attttgcata agttcagcaa
    22261 cacaaagccc ttgactcccg cttcgaaatt gaaaaccttg acactgttta tccatacagt
    22321 taaaaataat actgtataca aacacagtgt agagggactt ttatgcgt
//
LOCUS       DBBGLQ010000069        22291 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.069, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000069 DBBGLQ010000000
VERSION     DBBGLQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.069"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            271..942
                     /locus_tag="ACIYSB_003569"
     CDS             271..942
                     /locus_tag="ACIYSB_003569"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HGC5430535.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            952..2148
                     /gene="yaiP"
                     /locus_tag="ACIYSB_003570"
     CDS             952..2148
                     /gene="yaiP"
                     /locus_tag="ACIYSB_003570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGC5430536.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQPLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2126..2707
                     /locus_tag="ACIYSB_003571"
     CDS             2126..2707
                     /locus_tag="ACIYSB_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGC5430537.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2709..3482
                     /gene="yaiO"
                     /locus_tag="ACIYSB_003572"
     CDS             2709..3482
                     /gene="yaiO"
                     /locus_tag="ACIYSB_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HGC5430538.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDFNGLQLAARLTWKF"
     gene            3667..3942
                     /gene="frmR"
                     /locus_tag="ACIYSB_003573"
     CDS             3667..3942
                     /gene="frmR"
                     /locus_tag="ACIYSB_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGC5430539.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3977..5086
                     /gene="frmA"
                     /locus_tag="ACIYSB_003574"
     CDS             3977..5086
                     /gene="frmA"
                     /locus_tag="ACIYSB_003574"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGC5430540.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5180..6013
                     /gene="fghA"
                     /locus_tag="ACIYSB_003575"
     CDS             5180..6013
                     /gene="fghA"
                     /locus_tag="ACIYSB_003575"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGC5430541.1"
                     /translation="MELIEKHVSFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLAENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6237..6776)
                     /gene="yaiL"
                     /locus_tag="ACIYSB_003576"
     CDS             complement(6237..6776)
                     /gene="yaiL"
                     /locus_tag="ACIYSB_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGC5430542.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6878..8089)
                     /gene="mhpT"
                     /locus_tag="ACIYSB_003577"
     CDS             complement(6878..8089)
                     /gene="mhpT"
                     /locus_tag="ACIYSB_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter"
                     /protein_id="HGC5430543.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQSAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRIQPCADA"
     gene            complement(8253..8422)
                     /locus_tag="ACIYSB_003578"
                     /pseudo
     CDS             complement(8253..8422)
                     /locus_tag="ACIYSB_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8667..9680)
                     /gene="mhpE"
                     /locus_tag="ACIYSB_003579"
     CDS             complement(8667..9680)
                     /gene="mhpE"
                     /locus_tag="ACIYSB_003579"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGC5430544.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9677..10627)
                     /gene="mhpF"
                     /locus_tag="ACIYSB_003580"
     CDS             complement(9677..10627)
                     /gene="mhpF"
                     /locus_tag="ACIYSB_003580"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGC5430545.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10624..11433)
                     /gene="mhpD"
                     /locus_tag="ACIYSB_003581"
     CDS             complement(10624..11433)
                     /gene="mhpD"
                     /locus_tag="ACIYSB_003581"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGC5430546.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRTGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11443..12309)
                     /gene="mhpC"
                     /locus_tag="ACIYSB_003582"
     CDS             complement(11443..12309)
                     /gene="mhpC"
                     /locus_tag="ACIYSB_003582"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGC5430547.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSVVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(12327..13271)
                     /gene="mhpB"
                     /locus_tag="ACIYSB_003583"
     CDS             complement(12327..13271)
                     /gene="mhpB"
                     /locus_tag="ACIYSB_003583"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGC5430548.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(13273..14937)
                     /gene="mhpA"
                     /locus_tag="ACIYSB_003584"
     CDS             complement(13273..14937)
                     /gene="mhpA"
                     /locus_tag="ACIYSB_003584"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGC5430549.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            15128..15961
                     /gene="mhpR"
                     /locus_tag="ACIYSB_003585"
     CDS             15128..15961
                     /gene="mhpR"
                     /locus_tag="ACIYSB_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGC5430550.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGMHL
                     R"
     gene            16038..17120
                     /gene="lacI"
                     /locus_tag="ACIYSB_003586"
     CDS             16038..17120
                     /gene="lacI"
                     /locus_tag="ACIYSB_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGC5430551.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            17243..20317
                     /gene="lacZ"
                     /locus_tag="ACIYSB_003587"
     CDS             17243..20317
                     /gene="lacZ"
                     /locus_tag="ACIYSB_003587"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGC5430552.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            20369..21622
                     /gene="lacY"
                     /locus_tag="ACIYSB_003588"
     CDS             20369..21622
                     /gene="lacY"
                     /locus_tag="ACIYSB_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGC5430553.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21688..>22291
                     /gene="lacA"
                     /locus_tag="ACIYSB_003589"
     CDS             21688..>22291
                     /gene="lacA"
                     /locus_tag="ACIYSB_003589"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGC5430554.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVES"
BASE COUNT         5232 a         6027 c         5758 g         5274 t
ORIGIN      
        1 atatttttta aatgcaatta attgacaaac taaatcacat ctaaatacaa tgtataaaac
       61 aaaataacta tacataccaa acatacaaac acttaacata tgaattgcat agaaacatta
      121 cccagtcgaa cccacgggtc tgaaagccct tgcttttcag ttattagcaa catttaacat
      181 ttataaatta acatctggct gcgccagaga ttacgtctct gagtgcatcg ttgtgcttgt
      241 agtataataa ataaaatgga gtgtcattaa gtggataagg ttttagattc agccctcctt
      301 tcttcggcaa ataaaagaaa gggtatcctt gctattggcg cacatcctga cgatatagaa
      361 ttaggctgtg gcgcatcgct tgctcgtctt gcgcaaaaag gaatttatat cgcagccgtg
      421 gtgatgacta ccggcaactc tggcacagat ggaataatcg atcgccatga agaatcgcgc
      481 aacgccctaa agatattagg gtgccaccaa actattcatc ttaattttgc tgacacccgc
      541 gctcatttac agctcaatga tatgatttcc gccctggaag acatcattaa aaatcaaatt
      601 ccttctgatg ttgaaatcat gcgggtatat accatgcatg atgccgaccg ccatcaggat
      661 catcttgctg tttatcaagc ttcaatggtt gcctgccgaa ctattccgca aattctcggc
      721 tacgaaaccc caagtacctg gctttcattt atgcctcagg tttttgaatc cgttaaagaa
      781 gaatatttca cagtcaaact tgctgcatta aaaaaacata aaagccagga acgacgcgat
      841 tatatgcgtc atgatcgcct gcgtgcagtt gcacaatttc gcggacaaca ggtcaatagc
      901 gatctgggtg aaggctttgt aattcataaa atgattcttt gagcaaattg catgaaaacc
      961 tggatattta tctgtatgtc catagcaatg ttgctatggt ttttaagtac gctaagacgt
     1021 aaacccagtc aaaagaaagg ctgtattgac gccattatac ctgcgtataa cgaaggcccg
     1081 tgtctggcgc agtcactgga taatctactg cgtaaccctt atttttgccg ggtaatttgc
     1141 gttaacgacg gctccacgga caataccgaa gcggtcatgg cggaagtcaa acgcaaatgg
     1201 ggcgaccgct ttgttgccgt cacgcaaaaa aataccggta aaggtggtgc gctgatgaat
     1261 ggcctcaatt acgccacctg cgaccaggtt tttttaagtg atgccgacac ctatgttccg
     1321 cccgatcaag acggaatggg ctatatgctg gcagaaattg agcgcggtgc cgatgccgta
     1381 ggcggcattc cctctactgc gttgaaaggc gcgggtctgt taccgcacat ccgcgcgacc
     1441 gtaaagttgc cgatgattgt tatgaagcgc acgctacagc agcccctggg tggcgcaccg
     1501 tttattatca gcggtgcctg cgggatgttc cgtactgatg tattgcgtaa gttcggtttc
     1561 tcggatcgta ctaaagtcga agaccttgat ctcacctgga cattggtggc aaacggctac
     1621 cgtattcggc aggcgaatcg ctgcatcgta tacccacagg aatgcaacag cccgcgtgag
     1681 gagtggcgtc gctggcggcg ttggattgtg ggatacgcgg tctgtatgcg cctgcataaa
     1741 agacttttat ttagccgctt cggtatcttc agtatatttc ctatgctgtt ggttgtgctt
     1801 tatggcgttg ggatttatct cactacctgg tttaatgaat tcatcaccac cgggccgcat
     1861 ggagtggtgt tggcaatgtt tccgcttatc tgggtcggcg tagtttgtgt tattggtgct
     1921 tttagcgcct ggtttcatcg ttgctggttg ttggtgcctt tagcgccgct ttccgttgtg
     1981 tatgtattat tagcttatgc catctggatt atttatggac ttattgcctt ttttactgga
     2041 cgcgaacctc agcgcgacaa acccacccgc tattccgcac tggtggaagc gtcaaccgct
     2101 tattcccaac cttctgtcac aggaactgaa aaactatctg aagcttaatg ttaaagagaa
     2161 aaatattcag attgcagacc aggtaattat tgatgaaact gcaggtgaag ttgttatcgg
     2221 cgcgaatacc cgtatttgtc atggtgccgt tattcagggt ccggtagtga ttggcgcaaa
     2281 ctgcctgata ggtaattatg cgtttattcg tcctggcaca ataatcagca atggcgtaaa
     2341 aattggtttt gccaccgaga ttaaaaatgc ggttattgaa gcggaagcaa cgattggtcc
     2401 gcaatgtttt attgccgact cggtagttgc aaaccaggca tatttaggcg cacaagtacg
     2461 taccagtaat catcgtctgg atgaacagcc cgtgtctgtt cgaactccag agggaattat
     2521 cgctaccgga tgcgataaat taggttgtta tatcgggcag cgttcacgcc ttggcgtaca
     2581 agttattatt ttgcctgggc gaattatttc cccgaacacg caacttggcc cgcgcgtgat
     2641 tgtagaacgt aatttaccta ccggaactta ttcactccga caagaactta tccgtacagg
     2701 agattaaaat gataaaacgg acgttattag cggcggccat ttttagcgca ttgcccgctt
     2761 atgccgggtt aacttccatt accgcgggct acgattttac cgattattct ggcgatcatg
     2821 gcaaccgtaa tttagcgtat gctgaactgg tggcgaaagt tgaaaacgca acgctgcttt
     2881 ttaatctttc ccaggggcgt cgtgattatg aaactgaaca tttcaatgcc actcgcggtc
     2941 agggtgcggt ctggtataaa tggaataact ggctgacaac ccgaacgggt attgcctttg
     3001 cggataatac gccggtcttt gcccgccagg attttcgtca ggatattaac ctggccctgt
     3061 taccaaaaac gcttttcacg accggttatc gctacactaa atattacgat gatgtcgaag
     3121 tcgatgcctg gcaaggcggc gtatcactct atactggccc ggtgatcacc agctaccgct
     3181 atacccatta tgactccagc gatgcaggtg gtagttatag caatatgatt tccgtgcgtc
     3241 tgaatgaccc gcgcggcact ggttatacgc aactatggct aagccgcgga acaggtgctt
     3301 acacctatga ctggacgcca gaaacacgct acggcagcat gaagagcgtc agtctgcaac
     3361 gtattcaacc gctgactgag caacttaatc tcgggctgac ggcaggtaaa gtgtggtacg
     3421 acaccccaac cgatgatttt aacggtctgc aacttgcagc ccgtctgacc tggaaattct
     3481 gattccttct gccgcccgct atccggggcg gccttccctg ccgattagcc ccccctttcc
     3541 tctttgtttt ccgaccacat tcaccggata aattttattc tccagtgtta tatactatag
     3601 gggggtatgc attgacatat agaatacccc cctatagtat attgcatgca gatgatgagg
     3661 tgcgaaatgc ccagtactcc ggaagagaag aaaaaggtcc ttactcgagt tcgtcgtatt
     3721 cgggggcaga ttgatgctct ggaacggtcg ctggagggtg atgccgaatg ccgtgccata
     3781 ctccaacaga tcgctgccgt tcggggcgcg gctaatgggc tgatggcaga agtgcttgaa
     3841 agccatatcc gggaaacgtt tgaccgaaat gactgctaca gccgcgaagt cagccaatcc
     3901 gttgacgaca ctattgaact ggttcgtgcc tatcttaaat agctgaatct attaccatat
     3961 tgaggaagag cgagagatga aatcacgtgc tgccgttgca tttgctcccg gtaaaccgct
     4021 ggaaatcgtt gaaattgacg ttgcaccacc gaaaaaaggt gaagtgctaa ttaaagtcac
     4081 ccataccggc gtttgccata ccgacgcatt taccctctcc ggcgatgacc cggaaggtgt
     4141 attcccggtg gttctcggtc acgaaggggc cggcgttgtg gttgaagtcg gtgaaggcgt
     4201 aaccagcgtc aaacctggcg accatgtgat cccgctttac accgcggagt gcggcgagtg
     4261 tgagttctgt cgttctggca aaactaacct ctgtgttgcg gttcgcgaaa cccagggtaa
     4321 aggcctgatg ccagacggca ccacccgttt ttcttacaac gggcagccgc tttatcacta
     4381 catggggtgc tctacattca gtgaatacac cgtagtcgcg gaagtgtctc tggcaaaaat
     4441 taatccagaa gcaaaccatg aacacgtctg cctgctgggc tgtggcgtga ccaccggtat
     4501 tggcgcggta cacaacacag ctaaagtcca gccaggtgat tctgttgccg tgtttggtct
     4561 tggcgcgatt ggtctggcag tggttcaggg cgcgcgtcag gcgaaagcgg gtcggattat
     4621 cgctatcgat accaacccga agaaattcga tctggcgcgt cgcttcggtg ctaccgactg
     4681 cattaacccg aatgactacg acaaaccgat aaaagatgtc ctgttggata tcaacaaatg
     4741 gggtatcgac catacctttg aatgcatcgg taacgtcaac gtgatgcgtg cggcgctgga
     4801 aagtgcgcac cgcggctggg gtcagtcggt gatcatcggg gtcgcgggtg ccggtcagga
     4861 aatctccacc cgtccattcc agttggtcac tggtcgcgta tggaaaggtt ccgcgtttgg
     4921 cggcgtgaaa ggtcgttccc agttaccggg catggttgaa gatgcgatga aaggtgatat
     4981 cgatctggaa ccgtttgtca cgcataccat gagcctggat gaaattaatg acgccttcga
     5041 cctgatgcat gaaggcaaat ccattcgaac cgtaattcgt tactgatttc ccgcaggttt
     5101 accccgtcca cttcaggcgg gggttcttaa tactctcccc gggcagccgt ccgggggatt
     5161 aaccctgaga taatgactga tggaactcat tgaaaaacat gtcagctttg gcggctggca
     5221 aaatgtgtat cggcattatt cccaatcact gaaatgtgaa atgaatgtcg gcgtctatct
     5281 cccaccaaaa gccgcgaatg aaaaattgcc ggtgctgtac tggctttcag gcctgacctg
     5341 caacgagcag aatttcatta ctaaatcggg gatgcagcgt tacgcggctg agcacaacat
     5401 tattgttgtt gcgccggaca ccagtccgcg aggcagtcat gtcgcagatg ctgaccgtta
     5461 cgatctcggg caaggtgccg ggttttacct gaacgcgacg caagcgccgt ggaatgaaca
     5521 ttacaaaatg tatgactata tccgcaacga gctgccggat ttagtgatgc atcattttcc
     5581 ggcaacggcc aaaaagtcta tctctggtca ttctatgggc gggctgggcg cgctggtgct
     5641 ggcgttacgt aacccagatg aatatgtcag cgtctcggcg ttttcgccca ttgtctcccc
     5701 atcgcaagtg ccgtggggac agcaagcctt tgctgcatat cttgctgaaa ataaagatgc
     5761 ctggttggat tacgacccgg tgagtcttat ttcacaaggt caacgcgttg cggaaatcat
     5821 ggttgatcag gggttgagtg atgattttta cgcagaacag ctgcggactc caaatcttga
     5881 aaagatctgc caggagatga atatcaagac gttaatccgt tatcacgagg gttatgatca
     5941 cagctattat tttgtctcca gttttattgg cgagcatatt gcctaccacg ccaataaact
     6001 gaatatgcgt tgataatagc acgactgccg gatgcggcgt gaacgcctta tccggcctac
     6061 acttcgcccg taagccgtag gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc
     6121 acgactgccg gatgcggcgt gaacgcctta tccggtctac acttcgccca taaaccgtag
     6181 gcctgataag acgcgcatag cgtcgcatca ggcatctgcg cacgacttat ctatgtttac
     6241 cacatcaaat catcaggcac tttgaagtcg gcatacgggt cgtcttcatc ctgctcttcg
     6301 gcgctgagcg cgctgtgcaa cactatactg ctggcatcgc gctgagcaat tttatcggca
     6361 acgctggcgg gaataatggc gtattcacct tcgctattgt tatcgaccaa caagcgggca
     6421 atcgccagac gaccattaat caactgcgcc tgtgtgagct tatcgacaaa aatcttctta
     6481 atcagattgc cgtcagtgaa gttaaaacca atatcgccat tggcaatggt gattcggttc
     6541 atttcaataa gctgcttcac ctgagcttta tattctttcg ccaacgccgc ttgtttttgc
     6601 tgttcgctaa gctgtttatc acgctcaagc tgtgcctttt tattttcttc taccgccgcc
     6661 cgagcttcac gcgcctgaac gcgcgatttt ttcgccgttc tctccacctt cgccgctttt
     6721 ttgctggtga ctaatcctgc tttgagcaac tgctcttgta aggtaagttt tgccatcttt
     6781 gtttccaaac ccgctgaata atttttagcg agtatacctg taaacgccgt aggtgtattg
     6841 gggtgaagtc cctttcgccc ctgcctaata aggcacgtca ggcatcggcg cacggctgta
     6901 ttcgtgatct ccggctcatc aaaataaaca ccgccaaccc agcaacaaga atacccggtg
     6961 cagaagcggc cattacgccg accgtgccag tgcctaatgc cagcattttc ccggccagta
     7021 acggaccgct catagccccc agacgcccta ccgccacggc tgttcccaca cctgttgcgc
     7081 ggatctgcga actgtaaaac aacggtgcca gggcatacaa aacgctttgc ccacctgtcg
     7141 caaacaaccc cgcgacaaat cccgccagca acataccgtt aaatgacgac accgttccaa
     7201 gcgccagcag cgaagctaac atgccgctat aaatcagtag cgacatggtt actggacgca
     7261 gcttatccat caatgcgccc aacattaacg tcccgcttgc cgcccccatt tgcagggcaa
     7321 acatcacccc tgccgcctgc gatggctgga atccttgctc caccaaaagt agcggtagcc
     7381 agttgatcaa catgtagacc accagcagag tgaagaaata acacaaccac agcagcagcg
     7441 tcgcggttgc cgtttctggc gcaaataagg cacgcagtgg tggcgcagac tgtttttcgc
     7501 cagcgaaaac cgccgactcc ggcagccagc gcattaatag cggcaccaga atcaacggca
     7561 ccacaccacc tacccaaaac accgtttgcc atgctaagtt tgcccccgcg aaacccagtg
     7621 tcgccgccag cgccgcgcca atgggaacac cgcaatacat caggctcact gccgtcccac
     7681 gaaaacgtgg acccgcggct tcagacgtca gggcgataag attcggcaac gccgccccca
     7741 gcccgacacc ggtcatcagc cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg
     7801 ttgccagtga gaacaaacca aacagcgcaa ctgagccaat caaaatgcgc ttgcgaccat
     7861 aacggtccgc cagcattccg ccaaccaacg cgccgggtag caaaccgagt attccggcgc
     7921 taaatatcca gcccatttgc attttatcga gtgcgaaagc ctgggcgatg ccacccgccg
     7981 caatgccagc cgcctgaaga tccagccctt ccatcagagc gaccaaaaaa caaagcccga
     8041 tggtcagcat caggcgggat gaagatgatg aaggggtacg agtcgacata agatgaacct
     8101 cgttgcgagt gcaggtgaat cgcgactgat acattgccag ccgcgtaatc agaactattt
     8161 aatgactgcc tacgggcgaa atgtaggccg gacaagacgt ttatcgcatc cggcagtcgt
     8221 gtgcagatgc ctgatgcgac gctaacgcgt cttatcaggc ctacaaattc ccgcgccatc
     8281 cgtaggccgg ataaggcgtt tacgccgcat ccggcagtcg tgcgcagatg cctgatgcga
     8341 cgctaacgcg tcttatcagg cctacaaatt ctcgcgccac ccgtaggccg gataaggcgt
     8401 tcacgccgca tccggcagtc gtgcgcagat gcctgatgcg acgctaacgc gtcttatcag
     8461 gcctacaaat tcccgcgcca tccgtaggcc ggataaggcg ttcacgccgc atccggcagt
     8521 cgtgcgcaga tgcctgatgc gacgctaacg cgtcttatca gacctacaaa ttctcacgcc
     8581 atccgtaggc cggataaggc gttcacgccg catccggcag tcgtgctggg atgggcatac
     8641 aaaatgcacg ttctcggcag tcatcattat ttgttgttgc gcagatccag cgccacgtca
     8701 acgatcatat cctcctggcc gccaaccatc cggcgtttgc ccagctcaac gagaatatcc
     8761 accgcactta agccataacg cgccgccgcc gtttcacagt gacgcaggaa gctcgagtaa
     8821 acaccagcgt atcccagcgc cagcgtttcg cgatcgactc gtaccggtcg atcctgcaac
     8881 ggacgcacca ggtcgtcggc ggcatccatt aacgcataga gatcggtccc atgctgccag
     8941 cccagtttat ccgcggcggc aataaacact tccagcggtg cgttacctgc gcccgcgccc
     9001 attcccgcga ggctggcgtc gattcggtcg cagccctctt ccaccgccgc gatagagttc
     9061 gccacgccaa gactcaggtt atggtgagcg tgcatgccag tttgcgtttc tggtttcagc
     9121 tctgctttca gggcgcggaa acggtcacgg atatcgctca tgttcatcgc accgccagaa
     9181 tccaccacat aaatacaggt cgcaccgtag ccttccatca gctttgcctg cttggcgaga
     9241 ttctccggcg tggtcatatg gctcatcatc agaaaaccaa cggtgtccat tccgagctcg
     9301 cgggcatact gaatatgctg ggcggaaaca tcagcttcgg tacagtgcgt tgccacacga
     9361 accacccgcg cgccagcctg ccaggcattt ttcagatcgt gaatagtgcc aattcctggc
     9421 agcaacaacg tcgcgatttt ggcgtgcttc accacatccg ccgccgcttc aatccattca
     9481 aggtcgctat gtgcgccgaa accatagtta aagctggaac cttgcaaacc gtcgccgtgg
     9541 gccacttcaa tcgaatccac gcgggcatcg tccagtgctt tggcaatctg gcgaacgttt
     9601 tccagcgaat actgatgacg aatggcgtgc ataccgtcac gcaatgtgac gtccgagata
     9661 taaagttttt taccgttcat gccgcttctc ctgccttgcg cgccagtgac tgggccattt
     9721 tttccgctgt cgccagcgca ctggaagtca taatgtcgag gttgcccgca taggcaggca
     9781 gataatgcgc tgcgccttcg acttccagcc agaccgctgt tttcagtccg gagaattgcc
     9841 ccacgcccgg taaattgacc ggtttatcct gcgggataac ttcaaactgc acgcgctgtt
     9901 tcaggcgata acccggtacg taagcctgca ccgcctcagc catttcattg attgaggctt
     9961 cgatatcatc ttgtgaagct tcgtcgctca atacatacac cgtgtcacgc atcatcaacg
    10021 gtggctctgc tgggttaagc acaataatcg ccttcccttt tgccgcgccg cccaccactt
    10081 caatggctcg ggaagtggtt tccgtaaatt catcgatatt ggcacgcgtg ccaggtccgg
    10141 cagatttact ggcgatagaa gcgataattt cggcgtaatg aacacgcgcc acgcgtgaaa
    10201 ctgccgccac cattggaatg gtggcctggc cgccgcaggt gaccatgttg acgttcagtt
    10261 gatcgacgtt cgcctcgagg ttaaccaccg gcacgcagta agggccgatg gcagcaggcg
    10321 tcaggtcaat taagcgaata tccggtttcg cttcgcgtaa agcggcatcg tttttcacat
    10381 gagcaccggc gctggtcgca tcaaatacaa tgtcgatatc agcaaattca ggcatgttca
    10441 tcagtccgat caccccttca tgggtggtgg cgacgcccat acgtctggcg cgcgccagac
    10501 cgtcggactg aggatcaatg ccaaccatca ccgccatctc cagatgctga ccgtgacgca
    10561 aaattttaat catcagatcg gtaccaatgt tgccagaacc gataatggcg actttacgct
    10621 tactcatgac agacttcctt ttggggctgc gcttgaaaat gtcgcagcaa ctgaacctat
    10681 gccttcaata tgggcttcaa aacgatcgcc cgcattcacc gccaccatcg gacctaatgc
    10741 cccggtaaga atgatatctc cggtgcgcag cggttcaccc agactggcca ttttgcgtgc
    10801 cagccagacg gccgcattaa gcggatgtcc caggcattcg ctgccgcgcc cgctagaaac
    10861 ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc aggtctaacc ccgccggacg
    10921 ttgcgccgga ccgccgatga catacacccc acaggaggcg ttatctgcca cggtatcgac
    10981 aaactgaatc gaccagtcgc gaatgcggct ccccaccact tccagcgccg gaagtaccca
    11041 ttcaatggcg ttatacaatt cgtcgaaggt gatatcggtt gcgggcaaat cgcggttcaa
    11101 caccagtgcg atctccgctt caatgcgggg ttgcagaaca cgggaaaaag gaatgatttc
    11161 gttatcgcca taacacatgt cggcaaataa cgtcccaaaa tccggttgat caacgcccag
    11221 ttgttgttgc actttcggat gtgtcaggcc cactttacgc cctaccacgc gacgcccctg
    11281 cgcaacgtca tgttgcacat ttatgtgctg aatggcgtaa gccgcttcag cgttatcgat
    11341 accaatcaga tcgcgcagcg gtgcaatcgc ttcgccctgc tctgcggcgc ggcgtaaatc
    11401 cgccgccagt tgctcaagag tatgcttcgt catgaccatt ccttaagggc gtgcgaggaa
    11461 attcagcacc agttgattga aagcgtcggc atgttcccac tgcgcccagt gaccacagtc
    11521 gcggaagata tgcagttcag aaccggcaat gccggacagc agacgcagac ccgcatccat
    11581 cggcacaaag cggtcgttgc gcccccagac aatcagggtt tgcgctttga tttccgccag
    11641 acgtgggcca aaatccggga actgtttcgg attagcttcc aggctcttaa cgaagttttc
    11701 caggtgatcg cggcgcgaca gcatattatt caggcgcgct tcaaacaggg cgtcggtcaa
    11761 atcgctggta tcaaaaacga agatatccat catcagcttc aggttttcga tagtcggctg
    11821 acgataaagc tgattcagtc gcttaatacc ttcggttggc atcggcgtaa acaaactcat
    11881 gccgcccgta ccgccgccca tcagcaccag tttgccgacg cgctccggcc atttaagggt
    11941 gaacgccaca gaactatggc cccccatcga gttgcccagc aggtggattt tggcgatatc
    12001 cagttgatcc accacgcttt tcaggattcg tgcattaaga tccgatcgcg aaccactatt
    12061 aacgaccgaa tcgctcttgc cccaacccgg acaatccagc aggatcaccc gatagcccgc
    12121 ctctaccagc ggatcgatat tgcggctgaa gttcgcccag ccagtagcac ccgggccgga
    12181 accatgcagc aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat ggatgcgcag
    12241 cgttttaccc gcttcttcta cattcagaaa acggctggtg gcggcttcgg tttgtggctg
    12301 ataactcatc atcttctcct gcatattcag ttctctgttc tggcgcttaa cgagccaaat
    12361 ccggcaatcc actccgggat tgggcggtaa taacgccctt cgctacgcca gttgccaaac
    12421 gcagaaatag cggcaaaagc ggcgacccag gttttgattt catgtgtcga ctttccggca
    12481 atggcggaaa gctcttcgtt actgacggca tccagttcct gtatgcgtcc ctgctccagc
    12541 aaagtcatga actggttatc ccaaatcggg ttgagcggat gcagcgttct ctgatcctca
    12601 acaaacttct cagcggcgct aatcacccgt tgctgacgca attcgcgctc actggcgggt
    12661 aaatctttcc cgctccccaa cagacggtcg cgcatatggg catcggcttt cgccagttcg
    12721 ggcaccggcg gctgatggga aagcccaccg gaacccagga acagcacgcg tttattgaga
    12781 gtgctggtga aacgtccaat ggcttcaccc aacatgcggg tacgctggaa accgggcagc
    12841 ggcgtggcga caccgttgat gaacacaggc agaactggca ccttatccag cccaccgagc
    12901 aggaactcca gcggctgggc gaacccgtgg tccacctgca tacagtaaga aacggcaaga
    12961 tcgatcccgc tcttcatgac ggcatgcgca caggcctccg ccagctccac aggcacgggc
    13021 agctctcctg ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc taaacagaac
    13081 ggtggcatca cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag caccaccagt
    13141 tcaggggaga atgccgcaat acgctcgcgg gcgctggcaa tcacgccatt gacctcatcg
    13201 agcacctctt gcgccgggtc gacatacccc accagcggcg agtgggaaag acagtgaaga
    13261 taagcgtgca tatcaggcta ccttttcgac agaaacgtcg gcatcagggc gggtcagcgt
    13321 catcaccgac gccagtttat tcagggtctt gcccagggtt tgcggaatgg cggtggcggc
    13381 aacaaagcga tccgggcgca tcaccaccag cgaagcattg tgttgcgcga accagctacg
    13441 caggcgacct tgcgtatcgc ccacgcgtag tacgccgtcg tggttatcct gtgcggtatg
    13501 aatttgcact tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc actgctggat
    13561 ttgctcgtcg ctcatccccc acagtggatt gcatccccag ccaattaccg cgaagttcgc
    13621 gccgatcgcg ttatcgagca gcgtcacgtc gccgttttcc agcgtgactt tcggctgaat
    13681 aaacatcttg ccgaccggag agtgcttcgc ttcgccctca cgcatcagcg caccgccgta
    13741 atattgcggc atcggcttga agcgcatttc gaggaagtag cgttttactg gcggcagata
    13801 attcaacagc caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg gcggagccag
    13861 cacgttgccc gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc gacgttcttg
    13921 ttgataggta tcgagcagcg catcgcgggc tttcccctgg ataaccaacg ccagtttcca
    13981 tgcgaggtta aaggcgtcgc gcataccact gttatagccc tgcccctgcc ataccggcat
    14041 gatgtgcgcg gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt gcgccagtcg
    14101 cgcgttgtgg gtgtagacac gctggcgaat caattcaaca ttgtccggat taggcagcac
    14161 tttgcttaac agcttgcgca tattttgcgg ctcacgcagc tgctcttcgg tttctcccgg
    14221 catcaccata aattcaaagc gacgtaccgc atgaggcagc gcggcagaaa catacgggcg
    14281 caccggatcg caacacaaat agatatgcgg cgtacttaac ggatcgttgg cgatatctac
    14341 cacaatccac tgatttggcg cagttttacc ttcaaacggc acattcagag tgcgacggac
    14401 aaaacttgct ccaccgtcac aggctaccag ccactgggct ttgactattt cccgctgccc
    14461 ttctgccgtt ttcaggtgca aggtcacttc gtcatcttgc tgactgaagg cctccagctc
    14521 gcgggaaaac aagcagcgca cattcggaaa acgcgacacc ccttccagca tcaccgcatc
    14581 gacctgcggc tgaataaagg cgttacggcg cggccagcca aattcatcgg tcattggctg
    14641 aatatcagca aaacagcggc ctttcggggt gagaaaacgc atcgcgtgcc acggcgtagt
    14701 gtgcggcaga acatcatcga ccaggccgac cgactgcatg gtgcgcagcg cctcgtcatc
    14761 aataccaatc gcacgcgggt agtcgatcaa cttatcgagt ttctccacca ccagcacgtc
    14821 aatgcccatc tggccgagat agttcgccat catcagccca accgggccgg caccagcgat
    14881 cgccacctga acgctatggt taacagcagg ctggatgtca gggtgttgta ttgccatttc
    14941 agtacctcac gactcggaca aaatgtcgtt gcgcgcacag tacagcgcaa cttattttgt
    15001 taaaaacatg taaatgattt tttattgtgc gctcagtata ggaagggtgt tttcggctac
    15061 aatcaaaaca tgcccgaatg tgcaccaggt gcaccacgtt gttttaacta tagaaatgtc
    15121 aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct taacccgcgg
    15181 tctaatgtta ttaaatatgt taaataaact tgatggcggt gccagcgtcg ggctgctggc
    15241 ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgc tgcaggaaga
    15301 gggatatgtc cgccgtagcc cctccgatga tagttttcga ctgaccatca aagtgcggca
    15361 attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc cactgctggg
    15421 cgatctgttg cgcgaagtgg tatggccgac agatgtgtcc acgctggatg ttgatgcaat
    15481 ggtggtacgc gaaaccactc accgtttcag ccgcttatcc tttcaccggg caatggtcgg
    15541 gcgacgtttg ccgcttctga aaaccgcctc gggcctgacc tggctggcct tttgcccgga
    15601 acaagaccgc aaggaattaa tcgaaatgtt agcctcccgc cccggtgatg actatcaact
    15661 ggcacgggaa ccgttaaagc tggaagccat tctggcgcgc gcgcgcaaag agggttacgg
    15721 acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg taccgctgcg
    15781 cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg caatgaccat
    15841 tgaacaggca gcggaaaagc atcttccggc gctacaacgg gtagcaaaac agatcgaaga
    15901 aggggttgaa tcgcaggcta ttctggtggc cggaaggcga agcggcatgc atttacgttg
    15961 acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
    16021 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
    16081 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
    16141 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
    16201 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg
    16261 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg
    16321 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc
    16381 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg
    16441 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac
    16501 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg
    16561 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc
    16621 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg
    16681 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
    16741 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
    16801 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
    16861 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
    16921 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
    16981 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc
    17041 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    17101 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    17161 ccccaggctt tacactttat gcttccggct cgtatgttgt gtgaaattgt gagcggataa
    17221 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca
    17281 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
    17341 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    17401 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
    17461 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
    17521 gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc
    17581 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
    17641 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
    17701 gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga
    17761 cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag
    17821 tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
    17881 ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa
    17941 tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga
    18001 ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc
    18061 gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg
    18121 tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
    18181 ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt
    18241 ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg
    18301 aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
    18361 ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta
    18421 tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga
    18481 agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg
    18541 gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag
    18601 tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta
    18661 tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga
    18721 caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt
    18781 cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc
    18841 gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata
    18901 ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga
    18961 tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt
    19021 tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac
    19081 gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc
    19141 cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct
    19201 gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt
    19261 cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg
    19321 gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg
    19381 gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc
    19441 cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg
    19501 taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
    19561 taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga
    19621 cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc
    19681 ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga
    19741 tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag
    19801 ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt
    19861 ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc
    19921 agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc
    19981 cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc
    20041 gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg
    20101 cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca
    20161 tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg
    20221 gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg
    20281 tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca ggccatgtct
    20341 gcccgtattt cgcgtaagga aatccattat gtactattta aaaaacacaa acttttggat
    20401 gttcggttta ttctttttct tttacttttt tatcatggga gcctacttcc cgtttttccc
    20461 gatttggcta catgacatca accatatcag caaaagtgat acgggtatta tttttgccgc
    20521 tatttctctg ttctcgctat tattccaacc gctgtttggt ctgctttctg acaaactcgg
    20581 gctgcgcaaa tacctgctgt ggattattac cggcatgtta gtgatgtttg cgccgttctt
    20641 tatttttatc ttcgggccac tgttacaata caacatttta gtaggatcga ttgttggtgg
    20701 tatttatcta ggcttttgtt ttaacgccgg tgcgccagca gtagaggcat ttattgagaa
    20761 agtcagccgt cgcagtaatt tcgaatttgg tcgcgcgcgg atgtttggct gtgttggctg
    20821 ggcgctgtgt gcctcgattg tcggcatcat gttcaccatc aataatcagt ttgttttctg
    20881 gctgggctct ggctgtgcac tcatcctcgc cgttttactc tttttcgcca aaacggatgc
    20941 gccctcttct gccacggttg ccaatgcggt aggtgccaac cattcggcat ttagccttaa
    21001 gctggcactg gaactgttca gacagccaaa actgtggttt ttgtcactgt atgttattgg
    21061 cgtttcctgc acctacgatg tttttgacca acagtttgct aatttcttta cttcgttctt
    21121 tgctaccggt gaacagggta cgcgggtatt tggctacgta acgacaatgg gcgaattact
    21181 taacgcctcg attatgttct ttgcgccact gatcattaat cgcatcggtg ggaaaaacgc
    21241 cctgctgctg gctggcacta ttatgtctgt acgtattatt ggctcatcgt tcgccacctc
    21301 agcgctggaa gtggttattc tgaaaacgct gcatatgttt gaagtaccgt tcctgctggt
    21361 gggctgcttt aaatatatta ccagccagtt tgaagtgcgt ttttcagcga cgatttatct
    21421 ggtctgtttc tgcttcttta agcaactggc gatgattttt atgtctgtac tggcgggcaa
    21481 tatgtatgaa agcatcggtt tccagggcgc ttatctggtg ctgggtctgg tggcgctggg
    21541 cttcacctta atttccgtgt tcacgcttag cggccccggc ccgctttccc tgctgcgtcg
    21601 tcaggtgaat gaagtcgctt aagcaatcaa tgtcggatgc ggcgcgagcg ccttatccga
    21661 ccaacatatc ataacggagt gatcgcattg aacatgccaa tgaccgaaag aataagagca
    21721 ggcaagctat ttaccgatat gtgcgaaggc ttaccggaaa aaagacttcg tgggaaaacg
    21781 ttaatgtatg agtttaatca ctcgcatcca tcagaagttg aaaaaagaga aagcctgatt
    21841 aaagaaatgt ttgccacggt aggggaaaac gcctgggtag aaccgcctgt ctatttctct
    21901 tacggttcca acatccatat aggccgcaat ttttatgcaa atttcaattt aaccattgtc
    21961 gatgactaca cggtaacaat cggtgataac gtactgattg cacccaacgt tactctttcc
    22021 gttacgggac accctgtaca ccatgaattg agaaaaaacg gcgagatgta ctcttttccg
    22081 ataacgattg gcaataacgt ctggatcgga agtcatgtgg ttattaatcc aggcgtcacc
    22141 atcggggata attctgttat tggcgcgggt agtatcgtca caaaagacat tccaccaaac
    22201 gtcgtggcgg ctggcgttcc ttgtcgggtt attcgcgaaa taaacgaccg ggataagcac
    22261 tattatttca aagattataa agttgaatcg t
//
LOCUS       DBBGLQ010000070        22076 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.070, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000070 DBBGLQ010000000
VERSION     DBBGLQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.070"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            145..858
                     /gene="yghS"
                     /locus_tag="ACIYSB_003590"
     CDS             145..858
                     /gene="yghS"
                     /locus_tag="ACIYSB_003590"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGC5430555.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            890..1648
                     /locus_tag="ACIYSB_003591"
     CDS             890..1648
                     /locus_tag="ACIYSB_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430556.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1694..3043
                     /locus_tag="ACIYSB_003592"
     CDS             1694..3043
                     /locus_tag="ACIYSB_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGC5430557.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARYIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3043..3867
                     /locus_tag="ACIYSB_003593"
     CDS             3043..3867
                     /locus_tag="ACIYSB_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430558.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            3879..4439
                     /locus_tag="ACIYSB_003594"
     CDS             3879..4439
                     /locus_tag="ACIYSB_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGC5430559.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4470..5540
                     /gene="lptF"
                     /locus_tag="ACIYSB_003595"
     CDS             4470..5540
                     /gene="lptF"
                     /locus_tag="ACIYSB_003595"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGC5430560.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLIDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5537..6616
                     /gene="lptG"
                     /locus_tag="ACIYSB_003596"
     CDS             5537..6616
                     /gene="lptG"
                     /locus_tag="ACIYSB_003596"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGC5430561.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSMGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQH"
     gene            complement(6651..7823)
                     /locus_tag="ACIYSB_003597"
     CDS             complement(6651..7823)
                     /locus_tag="ACIYSB_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGC5430562.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7823..8071)
                     /locus_tag="ACIYSB_003598"
     CDS             complement(7823..8071)
                     /locus_tag="ACIYSB_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGC5430563.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8103..9017)
                     /locus_tag="ACIYSB_003599"
     CDS             complement(8103..9017)
                     /locus_tag="ACIYSB_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGC5430564.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETIQTQTYELCDGVAGGYDW
                     QRVQQLAADARCGSVRMVGIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(9014..10702)
                     /locus_tag="ACIYSB_003600"
     CDS             complement(9014..10702)
                     /locus_tag="ACIYSB_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HGC5430565.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSEFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHDNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            11112..12254
                     /gene="yghO"
                     /locus_tag="ACIYSB_003601"
     CDS             11112..12254
                     /gene="yghO"
                     /locus_tag="ACIYSB_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HGC5430566.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     HDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(12261..13025)
                     /gene="glcC"
                     /locus_tag="ACIYSB_003602"
     CDS             complement(12261..13025)
                     /gene="glcC"
                     /locus_tag="ACIYSB_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HGC5430567.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            13276..14775
                     /gene="glcD"
                     /locus_tag="ACIYSB_003603"
     CDS             13276..14775
                     /gene="glcD"
                     /locus_tag="ACIYSB_003603"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HGC5430568.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMEGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            14775..15827
                     /gene="glcE"
                     /locus_tag="ACIYSB_003604"
     CDS             14775..15827
                     /gene="glcE"
                     /locus_tag="ACIYSB_003604"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HGC5430569.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARVGTPLVTIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMVGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            15838..17061
                     /gene="glcF"
                     /locus_tag="ACIYSB_003605"
     CDS             15838..17061
                     /gene="glcF"
                     /locus_tag="ACIYSB_003605"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HGC5430570.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVSMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            17066..17470
                     /gene="glcG"
                     /locus_tag="ACIYSB_003606"
     CDS             17066..17470
                     /gene="glcG"
                     /locus_tag="ACIYSB_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HGC5430571.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            17492..19663
                     /gene="glcB"
                     /locus_tag="ACIYSB_003607"
     CDS             17492..19663
                     /gene="glcB"
                     /locus_tag="ACIYSB_003607"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HGC5430572.1"
                     /translation="MSQTITQSRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            20018..21700
                     /gene="glcA"
                     /locus_tag="ACIYSB_003608"
     CDS             20018..21700
                     /gene="glcA"
                     /locus_tag="ACIYSB_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HGC5430573.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         5271 a         5483 c         5927 g         5395 t
ORIGIN      
        1 aaaaatattt atttggttaa tatgttttta tgaaagcgta attcaggtca atgtcacaat
       61 aaaccattgt cacaataagg ttgaacggat attctgagca cggaccacgt aatattcaat
      121 acattattta ctgccgggtt tttcgtgaat caacgtaata tgtcaataat taattccacg
      181 ccagtgcgtg ttattgccat tgtaggatgt gatggttcag gtaaatcgac cctcacggca
      241 agcctggtaa atgaactggc agcaagaatg ccaacagaac acatttatct cgggcaatcg
      301 tccgggcgaa ttggcgaatg gatttcacag ctccctgtta ttggcgcacc ttttgggcgt
      361 tatctgcgaa gtaaagcggc acatgtgcac gaaaagccct caacaccgcc tggcaatatt
      421 actgcactgg ttatctatct gctttcctgc tggcgggcgt acaagtttcg caaaatgttg
      481 tgtaaaagcc agcaaggctt tctgctcatc accgaccgct acccgcaggt tgaagtgccg
      541 gggtttcgct ttgatggccc gcaattggca aaaaccacgg gcggtaacgg ttggataaaa
      601 atgttaaggc agcgcgagct gaagctgtac caatggatgg catcttattt gcccgtattg
      661 ttgattcgtc ttggcattga tgaacaaacc gcgtttgcgc gtaaacctga ccaccaactg
      721 gcagcgttac aggaaaaaat cgccgttacg ccgcaactga cattcaatgg cgcaaagatc
      781 cttgaactgg atgggcgaca ccccgccgat gaaattctgc aagcgtcact acgcgcaatt
      841 cacgccgcgc tttcctgatc accaaaatgg ataggggatg atttaacgaa tggatgcact
      901 acaaactcaa actgttaata gtacaaccgc gccgcagccc aactacattc cggggctgat
      961 tgcggtggtc gggtgtgatg gcaccggtaa atccacactg accaccgacc tggtgaaatc
     1021 gctgcaacaa cactggcaaa ccgagcggcg ctatctgggg ctgctctccg gcgaagacgg
     1081 cgacaaaatc aaacgattgc cgttggttgg cgtctggctg gaacggcgac tggcggccaa
     1141 atcctcgaaa acccaaagca tgaaaaccaa atctccggcg ctatgggcgg cggtgattat
     1201 gtactgcttc tcgctgcgaa gaatggcgaa tctacgcaag gttcagcgac tggcgcaaag
     1261 tggcgttctg gtggtcagcg atcgcttccc gcaggctgaa atttcgggct tttattatga
     1321 tggaccgggg attggcgtcg aacgtgcgac cgggaaaatc agcatgtttc tggcgcagcg
     1381 cgaacggcgt ttataccaac aaatggcgca atatcgcccg gaattaatta ttcgcctggg
     1441 cattgatatt gagactgcca tctcccgcaa gcctgaccat gactatgccg agctgcagga
     1501 caaaatcggt gtcatgtcga agattggcta taacggcaca aaaattcttg agattgattc
     1561 cagagcgccc tacagcgaag tgctggaaca ggcacagaag gcggtttctc tggttgccat
     1621 cgtttctgac cgccgaagtt taacttaagg ctggcgggat actttttact atcaacgata
     1681 aggtcaggct gaattggcgg gttttaacat caaacattgg tttgcagatg gcgcgtttcg
     1741 caccattatt cgcaatagcg cctggttagg ctccagtaat gtcgtgagcg ccttgctggg
     1801 tctgttggcg ctctcgtgtg ccggtaaagg gatgacgccc gccatgtttg gcgtactggt
     1861 gattgtgcaa tcgtacgcca agtcgatcag cgattttatt aagtttcaga catggcaact
     1921 ggtggttcag tacggaacac cagcattaac caacaataat ccgcagcaat tccgcaatgt
     1981 cgtctcattt tccttctcgc tggatatcgt cagcggcgcg gtggcgattg tcggtggcat
     2041 tgccttactg ccattccttt cccattcatt aggtctggat gaccaaagtt tttggctggc
     2101 agcgctctat tgcacgctca ttccttcaat ggcttcctcc acgccgaccg gcattctgcg
     2161 tgcggtagat cgcttcgatt taattgctgt acagcaggcg acgaaacctt ttctgcgcgc
     2221 agcggggagc gtcgtagcct ggtattttga ctttggtttt gcgggttttg ttattgcctg
     2281 gtacgtgtcg aatctggttg gcggcaccat gtactggtgg tttgccgcgc gcgaattacg
     2341 ccgccgaaat atccataacg ccttcaaatt gaatctgttt gagtctgccc gatacattaa
     2401 aggcgcgtgg agttttgtct ggtcaaccaa cattgcccac tccatctggt cggcgcgtaa
     2461 ctcgtgcagc acagtgttgg tggggatcgt gttaggaccc gctgccgccg ggttatttaa
     2521 aatcgccatg acattcttcg acgccgccgg aacgccagcg ggtttgctgg gtaaaagttt
     2581 ttacccagag gtgatgcgtt tagatccgcg caccaccaga ccgtggttgt taggtgtgaa
     2641 gtccggttta ctggcgggcg gaatcggtat tctggtggcg ctcgcggtgt tgatcgtcgg
     2701 caagccgctc atttcgctgg tgtttggcgt taagtatctc gaagcgtatg acctgattca
     2761 ggtgatgctg ggcgcaattg tgatttcgat gctgggcttc cctcaggagt cattgctatt
     2821 gatggcggga aaacagcgcg cttttctcgt ggcgcaaacc atcgcctcaa tcggctacat
     2881 cgtattgctg ttcatgttct gtcatctgtt tggtgtgctg ggagcagcgt ttgcttactt
     2941 cggtgggcaa tgtctggacg tggcgctctc gttaattcca accttaaagg cattttttca
     3001 gcgccattcc ttgctttata acgcagccgg agagaaatcc tgatgatgag aaaatatttc
     3061 ccgcttgagg cgagcgagcg tctatttgtt gcgattgaag aagatgatgt cgttgatgca
     3121 caggttagtt tgccgccgac tattgcacta agttgtacca ccgaaatcat tcatgataat
     3181 tacgcgttat gcctgcaatt ctggctcaat ggcgttgatc gtcaggagtt actgcgttta
     3241 gttcgcaaac aagccaaagg cgatgagctg actgctgatg agcgcaaaca atttaaatac
     3301 atgcgagcgc gctacaagca cctgcgtttt gcgcaacggc tgtatttaaa gaagcatcag
     3361 gccggatttt tattcggcaa aacgaccgtt tttctggggc gttttcagga cggctttcgt
     3421 aacggtaaga aaaatattgt gtcgtattac ggcaacctgt tacggattta tttaagctct
     3481 ccggtctggt cgttggtgaa ctattcatta cgccatagcc agttagaaag cgtaagcagt
     3541 tttattgcct atcggcaaaa gcaaatgcat accctgaaag agattattgc caaaccgcga
     3601 ctaaccggta gggaatttca tgatgtgcga aaaatcatca gccagcaggt ttcttattac
     3661 gacaccttaa gatcgcttga tcctgaaaat aaagaggcgt tacagatatc ccgcttcctc
     3721 gccgccatta atggcctgat gggagataaa cacgacgaca tggtggcgga tgatatggaa
     3781 aaccgccagt cgtatgatgc accggtggcg ctggatagcg atattcgtca gcgcttagag
     3841 ctgctgattt cgcgttttcc gctatagccg gagcaatgat gtcatctcgt ttgattattg
     3901 cgttgattat catgctctta gccccgggtg tgcaggcaca caatttcgtc actggcaaaa
     3961 cggttacccc tgtttatatc caggaaggcg gcgagctgct tctgaatagc gacgacgaaa
     4021 ttcactacca aaagtggaac agcacacaat tagcgggaaa agtccgcatt attcagtata
     4081 tcgccgggcg taagtcggcg aagaaaaaga actcactgtt aattaaagcg gttgaagcgg
     4141 caaactttcc gcaggatcgt tttcagccca ccaccattgt gaacaccgat gatgccatct
     4201 ttggtactgg ctattttgtt gttggcaaaa tcgaaaaaaa taaacgccgc tatccgtggg
     4261 cgcaattcgt tattgacggc aatggacagg gacgcgttgc ctggcgcttg ccggagcaga
     4321 gttcaaccat tctggtgctg aataaagccg ggcaaattca gtgggcaaag gacggcagcc
     4381 tgacgccgga agaggtagat catgtcattg cgttggcgca gaaactgatc aatgaataaa
     4441 aaactcaaag gattgagggc gttagtgaga tgaagctggt tgaacactac atcatgcgtg
     4501 gtacgcgccg tctggtgctg attatcgtcg gttttctgat ctttattttc gccagctact
     4561 ccgcacagcg ttatctgacc gaagcggcaa atgggacatt agctcttgat gttgtgctgg
     4621 atatcgtttt ctacaaagta ctgattgcac tggagatgtt gttacctgtt ggtctgtatg
     4681 tgtcagttgg cgtgacgcta gggcagatgt ataccgactc ggaaattacc gctatctctg
     4741 cggcgggggg cagtccggga cgcttgtaca aagccgttct ttatctggcg ataccgctaa
     4801 gtatttttgt cacccttctg tcgatgtatg gtcgtccgtg ggcttatgcg cagatttatc
     4861 aactggagca gcagtcgcag tcggagctgg atgttcgcca gttgcgggca aagaaattta
     4921 acactaacga taacggacga atgatccttt cgcagacggt tgatcaggat aataatcgcc
     4981 tgactgacgc gctgatttat acttctactg ccaatcgaac ccgcattttc cgcgcccgtt
     5041 cggttgatgt ggttgaccca tcacccgaga aaccgaccgt tatgttgcac aacggcactg
     5101 cctatcttat cgatcatcag gggcgtgatg acaacgaaca gatctaccgt aatctgcaat
     5161 tacatctgaa tccgctggat caaagcccta acgtcaaacg caaagcgaaa tcggtcacgg
     5221 agctggcgcg ctccgtcttt ccagccgatc atgccgaact gcaatggcgg caaagccgtg
     5281 gcctgaccgc attgttgatg gcgctgctgg ccatttcatt aagtcgggta aaaccgcggc
     5341 aagggcgatt ttcaacgtta ttgccactga cgttgctgtt tattgccatt ttttatggcg
     5401 gtgacgtctg ccgtacgctg gtggctaacg gcgcgattcc cctcattcct ggtttgtggt
     5461 tagtacccgg actcatgcta atgggcctgc tgatgctggt cgcacgcgac ttctctttgc
     5521 tgcagaaatt ttcccgatga atgttttcag ccgctattta atccgtcatc tctttctcgg
     5581 ttttgccgcc gccgcagggc tattgctgcc gctttttacc accttcaacc tgattaacga
     5641 actggatgat gtcagcccgg gcggttatcg ctggactcag gcggtgctgg tggtgctaat
     5701 gaccttaccg cgcacactgg tcgaactttc gccatttatc gccttattgg gagggattgt
     5761 cggcctgggg cagttatcga aaaacagtga gcttaccgcc attcgcagca tggggttttc
     5821 tatcttccgt attgcactgg tggcgctggt tgcagggata ttgtggactg tttcgttagg
     5881 cgcgatagat gagtgggtag cgtcgccatt acagcaacag gcgttgcaaa tcaaatcgac
     5941 cgccaccgcg ttgggggagg acgatgacat taccggcaat atgctgtggg ccaggcgtgg
     6001 taatgaattt gtgacggtga aatcgctgaa cgagcagggc cagccggtgg gcgtggagat
     6061 ttttcattat cgcgacgatc tctcgctcga atcctacatt tatgcacgca gtgccaccat
     6121 tgaagacgac aaaacgtgga tcctgcatgg tgtgaatcat aaaaaatggc tgaatggcaa
     6181 agaaacgctg gaaacattag ataatcttgc ctggcaatcg gccttcacca gtatggatct
     6241 tgaagagtta tcaatgccgg gcaatacttt ttctgtccgt cagcttaatc attacatcca
     6301 ttatttgcag gaaaccgggc aacccagcag cgaataccgc cttgcactgt gggaaaaact
     6361 ggggcaaccc atcctgaccc tggcgatgat tttgctggct gtgccgttta cctttagcgc
     6421 cccgcgctcg ccagggatgg gtagccgtct cgctgtaggt gtcatcgttg gcttactcac
     6481 ctggatcagc tatcaaatca tggtcaatct gggattgcta tttgcgttga gcgcacctgt
     6541 taccgcgctc ggtttaccga tagcgtttgt gctggtggcg ttgagcctgg tgtattggta
     6601 tgacagacaa cattaaaccc gccgtggcgc aacaccacgg cgggctcaca ttactgcttc
     6661 agagtagcaa atgcctgaat gatggcatcg atttgctcac gactgtgagc agcgtttacg
     6721 ctacagcgca gcagcgtgat gcctgcaggc gcggcaggtg gcaggataag attcacataa
     6781 acgcccagcg aaataagctc gcgccagatg cgtaaaccgt cctctttcga accgattatt
     6841 accggcacta ccgggctaat atgtgaactt aactcataac ccagtttttg cagaccgtca
     6901 tacagatggt tggcattatc catcagcttt tgccgcagct ctggttgtgt ggcaatagtt
     6961 cttaacgacg agcgcacggt cgcaatgcag gacggagaag gagaggcggt aaaaatatac
     7021 gggcggctgc cgtagcgcag cacttccatc gcttccgaac cgacagcaaa accgccaatc
     7081 gaggccagac ttttactgaa ggtgccaacg ataatatcca catcatcttc aacgccgaca
     7141 gcttcagcca gtccgcgccc cgttgcgcct aacacgccaa acgaatgcgc ttcatcgaca
     7201 atcaaataac caccgagacg acgtttgata tcaacaattt ccgctaacgg cgcaacgtca
     7261 ccgagcatgc tgtagatacc ctcaacgatg ataatcgcct ctttcgcccg ttcaccgagg
     7321 cgaaccatgc gccgttccag atctttagca tcgttatggc ggaaacgaat aatttccgcg
     7381 ccaccgagag aacaggcgtc ataaatactg gcgtggctgt cggcatccag taacaccacg
     7441 gcgttatggt cggcaagtgc gctgatgacg cctaaattag cggtataacc ggttgaaaaa
     7501 acgatagcgg tagggcgatt gaaaaactcg gcaatctctt tctctaacgc caggtgcggc
     7561 gcataactgc cgtttgccat acgagaaccg gtggtgccgg taccttgggc cgctaacgcg
     7621 gcctgacctt cgctgatggc atcatgatta aaggttaaac cgagataatt attggtcccg
     7681 gccagaatca cttttttatt gccgatccgg ccttcggtgg cggaatacac ttcatcaata
     7741 caggtgccaa acggtgttaa cccgttgtca ctaaattgca gtcgctcgcc agcaaggcgc
     7801 gcatatttat cgtatagccc cattatttat tctccagcca tggaagtaga gtttccatta
     7861 attgcgcagg ggttttgaca tccagcagaa tattaatagg aatggaaata tcgaatctgt
     7921 cttccagcat cattaagaga tccatcactt tgatcgactc caggccaaga tcgttgacga
     7981 gatcgctgtc gggcttaatt tccacaccgt tttcgaccaa atcctgtaaa caggaaagga
     8041 tgtaatccat tactatttca cgatttacca tagaaacgtt aacacacctt taaacaagac
     8101 aatcaaaata gcccgttgcg taatgcgtgt tccaggctaa tcccgggaaa ccaattaata
     8161 tcttcagaaa tacggttatt acttgccgac cagtcggcgt gggttaattc acgaattttc
     8221 gagcgggtca gcataggttc tttacctgcc aggcgactca acgcggtact gatatccgca
     8281 aggccggtga gtaccggcag aggaatgcca accattcgca cggaaccgca acgggcgtcg
     8341 gcggcaagtt gctgtacgcg ttgccagtca tagccgccgg cgacgccatc gcataattca
     8401 taggtttgcg tctgtatagt ttcggcgctt aaccactgac ccactgcttg cgcgaaatcg
     8461 gtgacgtgca gaaaagagag ctgtgtatct ggcgcaccaa gtcgtggcag caggccgcgc
     8521 agcatccagt caaacagcgg ttttaactct ttatcgccgg gaccatacac ggctgtcggg
     8581 cgaaaaacgc ccagcgtaat ttcgtccgcc attgctgcca gtcgttgctc ggcgacgtat
     8641 ttggattttg cataccagga gagctcggga tggcgcgccg ccagcgaaga gataaacaga
     8701 aaacgttggc aaaagccgct ctcttttgct gcttgcatca ggcgcaggct gccgtcaacg
     8761 ttacagcggg tgaaaatctc ttctttgtgc ccgcgcactt gcccggcgca atggaccact
     8821 acgctggctc cggcaaccag ctcgctaagt gaatgagtat cttccagcga accgcgcacc
     8881 caggtgagat tatcattgac gtgagcgcgg gcagtacgcg tcaatgcgcg aacgtgaaag
     8941 ccgcgggcga gcaggttatc gataatatat ttaccgataa acccggtagc gcccgtcacc
     9001 gcgacagttt gattcatgcc agggattcct gcacattaag actggcagca taagccttct
     9061 gataacgttt tttcgcttcc gcacgggcag gcttgccgga ggacgttcgg ggaatactgt
     9121 ggggcggcaa cagatcgata gccgcggtca cgccaaattc gctttggatc cgtgccgcca
     9181 gcgcgtggat aagctgcccg cgacgttctt cgtcgctgat ccgacactgg atctgcaaaa
     9241 tgattttttc ctgggcggta acaaaagcaa tcgcatcgcc agaatgaatt tccggttcct
     9301 gttccgctat atattcaata tcctgcggcc agatattacg gccacgaata ataatcagat
     9361 ctttaatgcg tccggtgaca tacagataac cgtccagcag ataaccgagg tcgccggtgt
     9421 ctaaccagcc cgtcgcggca atctcgtctt gcgaaacctg gtcgccaaag taaccgctca
     9481 tcagactggg accggagatg caaatatggc ctaccacacg ttccgcgacc ggcatacctg
     9541 cttcattgcg gatttcaata ccatgttccg gcaacgcttt gccgcagttg acgaaagtcg
     9601 atacggcgcg tgtctctgca cccggcgcga cggctttacc ctgatattcg aggatgtcgc
     9661 gatccacttc gttaaccaca accccggagg cttcatcaga gaagctgaca gccagcgcat
     9721 tttctgccag tccgtagcac ggcatgaaag ttttattgtc aaagttaacc tgacggaaac
     9781 attcagcgaa ttgatggagt tgttctgcgg agatgggttc tgcaccaata ccagcgacgc
     9841 gccagcagga aagatccagt tcagcgagat ctttttcatt cacgcggcgc tggcacaatt
     9901 catagccaaa cggcggcgca acggaaacgg tgccgcgatt tttactgatc aatttaagcc
     9961 attgcagagg acgcatggca aaatcctgag tgcgcaaata atctactgaa agctgcgtgg
    10021 cgacgggggt caggagaaag ccgaccagtc ccatatcatg gtagaaaggc agccaggaga
    10081 cgcagcggtc gccagggcgt aatttaatgc cgtcgtggct tatagcacgt agattagcca
    10141 tcacttcgcg atgggtgata atgacgccac ggggaaaacg ggtgctgccg gaggtgtact
    10201 ggaggtaggc gatatcgttc ggaactggac gctggagcgc aacatcggct tccggtaatg
    10261 ccttaaacca ggcgtggctt aaaacatgta attcggggtt gtcatgcgtc gcggcattga
    10321 ccagtggcaa ccactcatca ccagtgataa tggctgcggg ctggcaactt gccagtaaac
    10381 cctgcaattt ggcgctccag gaatcccgct gaccaacgcc cattggaatc gccaacggga
    10441 cggcgactaa gccggcatac tggcaggcaa aaaaagcctc tacgaactcg ctacttgttt
    10501 cggcaatcag tgccacgcga tcgccttttt tcaggttcag cgataacaac cgcttcgcac
    10561 cagcttcggc acgtgctttt aacgtctgat attccagttg atcttcaagt tggcaacgtc
    10621 tgtcataaaa attcattccg gcgctactca gagcggcgta gtccaaagca tcaacaagcg
    10681 ttggaaaatc ggcatagcgc ataggtaggg aatgcgtaaa gattttatta gacatataca
    10741 ccacgaaaaa attggatatc caaaataaaa tgacaataat gcggtggatt atataggcat
    10801 aaaagataaa tgagatagta ttttcatcag tgtgaaaatg aacattgatt taaataatga
    10861 gtttgagttt ttttgttttt attgtgaatg atatttaatt tatgaatata ttaagaagaa
    10921 gccttgtccg gtcgccttaa atcgacaaaa ggcattgtgg cgcttctgtg tagtctctga
    10981 ccttatcgta atcattaata aatactattt attcgtcgtt tgtattcatt gggttgatta
    11041 ttaaggtgcc gttcgttgct gttgcaacct gtttgtcacc gttaattatt tgttacccac
    11101 agaacagaac tatggaatgc gatctgctga tgattaaaat tgaaaaagta attaataaaa
    11161 atgaccttaa ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga
    11221 tacccccttt attcattgaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc
    11281 atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg
    11341 cgcaaataga taccttgcac cgcgagcgtt acggcaaaga taccggtcat ttcggcatga
    11401 ttgacgccat tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt
    11461 tgaagtcaca aggtgcaagt aagatcagcg gtcctttcag cctgaatatc aatcaggaaa
    11521 gcggattact gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac
    11581 cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt
    11641 ggtggatgca gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg
    11701 tgcgcaaaaa ggtgaccatt cgctgcatca atcgtcagcg gtttgccgaa gagatgcaga
    11761 tcctgcgtga gattttcaac tctggctggc agcacaactg gggatttgtg ccgtttaccg
    11821 aacatgaatt cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct
    11881 atatcgctga gattgattct gcaccctgcg cgtttattgt cggcttgccg aacatcaacg
    11941 aggcgattgc cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc
    12001 gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt
    12061 atcagttcag ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacacgatc
    12121 cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag
    12181 gcatgaataa catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg
    12241 aaaagcagat ttaataccag ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc
    12301 aaatggtggc cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatcg
    12361 cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta gatccgcgca
    12421 tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca
    12481 aacactgaat taaacatcag atcggtcaat gattgcagcg taaacaccag cacctgattg
    12541 tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc
    12601 gaaatctctt tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca
    12661 acaaaatcag cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc ctccagtaat
    12721 gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tactgaacag atggatcagc
    12781 gggctggtgt cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtt
    12841 tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag
    12901 ccgagctttt cacacagtcg acgttccgag ggaagcggct gaccgacctt cagtacgccg
    12961 tcgataatta accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct
    13021 ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgttcttaac tcgcaaaaca
    13081 cgcacatcac gtaagtgatt atgttacatc aatttaacat tgagttaacc aagacaaggt
    13141 cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag
    13201 cccgcttaat aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaaca
    13261 aagcgaagcc tactcatgag catcttgtac gaagagcgtc ttgatggcgc tttacccgat
    13321 gtcgaccgca catcggtact gatggcactg cgtgagcatg tccctggact tgagatcctg
    13381 cataccgatg aggagatcat tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt
    13441 ccattactgg ttgttctgcc taagcaaatg gaacaggtga cagcgattct ggctgtctgc
    13501 catcgcctgc gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg
    13561 ctgccgctgg aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat cctcgacatt
    13621 aaccccgttg gtcgccgcgc gcgcgtgcag ccaggcgtgc gtaacctggc gatctcccag
    13681 gccgttgcac cgcataatct ctactacgca ccggaccctt cctcacaaat cgcctgttcc
    13741 attggcggca atgtggctga aaatgccggc ggcgtccact gcctgaaata tggtctgacc
    13801 gtacataacc tgctgaaaat tgaagtgcaa acgctggacg gcgaggcact gacgcttgga
    13861 tcggacgcgc tggattcacc tggttttgac ctgctggcgc tgttcaccgg atcggaaggt
    13921 atgctcggcg tgaccaccga agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg
    13981 gttctgttag ccagctttga ctcggtagaa aaagccggac ttgcggttgg tgacatcatc
    14041 gccaatggca ttatccccgg cgggctggag atgatggata acctgtcgat ccgcgcggcg
    14101 gaagatttta ttcatgccgg ttatcccgtc gacgccgaag cgattttgtt atgcgagctg
    14161 gacggcgtgg agtctgacgt acaggaagac tgcgagcggg ttaacgacat cttgttgaaa
    14221 gcgggcgcga ctgacgtccg tctggcacag gacgaagcag agcgcgtacg tttctgggcc
    14281 ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc cggattacta ctgcatggag
    14341 ggcaccatcc cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg tttatcgcag
    14401 caatatgatt tacgtgttgc caacgtcttt catgccggag atggcaacat gcacccgtta
    14461 atccttttcg atgccaacga acccggtgaa tttgcccgcg cggaagagct gggcgggaag
    14521 atcctcgaac tctgcgttga agttggcggc agcatcagtg gcgaacatgg catcgggcga
    14581 gaaaaaatca atcaaatgtg cgcccagttc aacagcgatg aaatcacgac cttccatgcg
    14641 gtcaaggcgg cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta
    14701 caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc tttccctgaa
    14761 ctggagcgtt tctgatgcta cgcgagtgtg attacagcca ggcgctgctg gagcaggtga
    14821 atcaggcgat tagcgataaa acgccgctgg tgattcaggg cagcaatagc aaagcctttt
    14881 taggtcgccc tgtcaccggg caaacgctgg atgttcgttg tcatcgcggc attgttaatt
    14941 acgacccgac cgagctggtg ataaccgcgc gtgtcggaac gccgctggtg acaattgaag
    15001 cggcgctgga aagcgcgggg caaatgctcc cctgtgagcc gccgcattat ggtgaagaag
    15061 ccacctgggg cgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt
    15121 cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg cgctggaaaa catctgcgtt
    15181 ttggtggcga agtgatgaaa aacgttgccg gatacgatct ctcacggtta atggtcggaa
    15241 gctacggttg tcttggcgtg ctcactgaaa tctcaatgaa agtgttaccg cgaccgcgcg
    15301 cctccctgag cctgcgtcgg gaaatcagcc tgcaagaagc catgagtgaa atcgccgagt
    15361 ggcaactcca gccattaccc attagtggct tatgttactt cgacaatgcg ttgtggatcc
    15421 gccttgaggg cggcgaagga tcggtaaaag cagcgcgtga actgctgggt ggcgaagagg
    15481 ttgccggtca gttctggcag caattgcgtg aacaacaact gccgttcttc tcgttaccag
    15541 gtaccttatg gcgcatttca ttacccagtg atgcgccgat gatggattta cccggcgagc
    15601 aactgatcga ctggggcggg gcgttacgct ggctgaaatc gacagccgag gacaatcaaa
    15661 tccatcgcat cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc ggagatggtg
    15721 gctttgcccc gctatcggct cctttattcc gctatcacca gcagcttaaa cagcagctcg
    15781 acccttgcgg cgtgtttaac cccggtcgca tgtacgcgga actttgagga gcaggctatg
    15841 caaacccaat taactgaaga gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc
    15901 ctgcgcgcct gtgttcactg cggattttgt accgcaacct gcccaaccta tcagcttctg
    15961 ggcgatgaac tggacgggcc gcgcgggcgc atctatctga ttaaacaggt gctggaaggc
    16021 aacgaagtca cgcttaaaac acaggagcat ctcgatcgct gcctcacttg ccgtaattgt
    16081 gaaaccacct gtccttctgg tgtgcgctat cacaatttgc tggatatcgg gcgtgatatt
    16141 gtcgagcaga aagtgaaacg cccactgccg gagcgaatac tgcgcgaagg attgcgccag
    16201 gtagtgccgc gtccggcggt cttccgtgcg ctgacgcagg tagggctggt gctgcgaccg
    16261 tttttaccgg aacaggtcag agcaaaactg cctgctgaaa cggtgaaagc taaaccgcgt
    16321 ccgccgctgc gccataagcg tcgggtttca atgttggaag gctgcgccca gcctacgctt
    16381 tcgcccaaca ccaacgcggc aactgcgcga gtgctggatc gtctggggat cagcgtcatg
    16441 ccagctaacg aagcaggctg ttgtggcgcg gtggactatc atcttaatgc gcaggagaaa
    16501 gggctggcac gggcgcgcaa taatattgat gcctggtggc ccgcgattga agcaggtgcc
    16561 gaggcaattt tgcaaaccgc cagcggctgc ggcgcgtttg tcaaagagta tgggcagatg
    16621 ctgaaaaacg atgcgttata tgccgataaa gcgcgtcagg tcagtgaact ggcggtcgat
    16681 ttagtcgaac ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg cgataaaaag
    16741 ctggccttcc actgtccgtg taccctacaa catgcgcaaa agctgaacgg cgaagtggaa
    16801 aaagtgttgc ttcgtcttgg atttacctta acggacgttc ccgacagcca tctgtgctgc
    16861 ggttcagcgg gaacatatgc gttaacgcat cccgatctgg cacgccagct gcgggataac
    16921 aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc
    16981 cagacgcatc tggcgagcgc cggtcgtacc tctgtgcgtc actggattga aattgtagaa
    17041 caagcccttg aaaaggaata acaaaatgaa aactaaagtc attcttagcc agcaaatggc
    17101 gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa aataactggt ctgtttccat
    17161 tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt cgcatggacg attgcgcgcc
    17221 gattgcggct tatatctccc aggagaaagc gcgtaccgcc gcgctggggc gtcgtgaaac
    17281 taagggctat gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg cgccgttatt
    17341 aacgtcgctg gaaggcggcg taccggttgt tgtggatggg caaattattg gtgccgtggg
    17401 cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt
    17461 ggcgaaataa gcgaaaacga ggagataaac aatgagtcaa accataaccc agagccgttt
    17521 acgcattgac gccaatttta aacgttttgt ggatgaagaa gttttaccgg gaacagggct
    17581 ggacgctgcg gcgttctggc gcaattttga tgagatcgtt catgatctgg caccagaaaa
    17641 tcgtcagttg ctggcagaac gcgatcgcat tcaggcagcg cttgatgagt ggcatcgcag
    17701 caatccgggg ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg aactgggcta
    17761 cctggtgccg caaccggagc gcgtgacggt ggaaaccacg ggcattgaca gcgaaatcac
    17821 cagccaggcg gggccgcagc tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc
    17881 ggcgaacgct cgctggggct cactgtacga tgcgttatac ggcagcgaca tcatcccgca
    17941 ggaaggggcg atggtcagcg gctacgatcc gcaacgcggt gagcaggtta tcgcctgggt
    18001 tcggcgtttc ctcgatgaat ctctaccgct ggaaaacggc agctatcagg atgtggtggc
    18061 gtttaaggtg gttgataaac aattacgcat ccagttgaaa aatggtaaag aaaccacgtt
    18121 acgtactcca gcacagtttg tcggttaccg tggcgatgcc gctgcgccga cctgcatttt
    18181 gctgaaaaat aacggcctgc atattgagct gcaaatcgat gccaatgggc ggattggcaa
    18241 agacgatccg gcgcacatca acgatgttat cgtcgaagct gctatcagta ccattctcga
    18301 ctgcgaagat tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt accgcaacct
    18361 gctgggcctg atgcagggga ctctgcaaga gaaaatggag aaaaacggtc ggcaaatcgt
    18421 gcgtaaactg aatgacgatc gtcattacac cgccgccgat ggctctgaaa tttctctgca
    18481 cggacgctcg ctgctgttta tccgcaacgt gggtcatttg atgaccattc ctgtgatttg
    18541 ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga ctggcgcgat
    18601 tgccctctat gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct atattgtgaa
    18661 accgaaaatg cacggtccgc aggaagtggc gttcgccaac aaactgttta cccgcattga
    18721 gacaatgctc ggtatggcac cgaataccct gaaaatgggc attatggatg aagaacgtcg
    18781 gacctcgctg aacttgcgta gctgtatcgc tcaggcgcgc aaccgcgtgg cgttcatcaa
    18841 taccggtttc ctcgaccgta ccggcgatga aatgcattcg gtgatggaag ctggcccgat
    18901 gctgcgtaaa aatcagatga aatcgacgcc ttggatcaaa gcctacgagc gtaataacgt
    18961 gctttccggt ctgttctgtg ggctgcgcgg taaagcgcaa attggtaaag gcatgtgggc
    19021 aatgccggac ctgatggcag acatgtacag ccagaagggc gaccaactgc gtgccggggc
    19081 aaacacagcc tgggttccgt caccaaccgc tgctacgctc catgcgctgc actaccacca
    19141 aaccaacgta cagagcgtac aagccaacat tgcccagacc gagttcaatg ctgaatttga
    19201 accgctgctg gacgatctgc tgactattcc ggttgctgaa aacgctaact ggtcggcgca
    19261 agagatccaa caagagctgg ataacaacgt gcaggggatt ctggggtacg tggtgcgctg
    19321 ggtggagcag gggattggtt gttcaaaagt gccggatatt cacaatgtgg cgttgatgga
    19381 agaccgcgca acgctgcgta tctccagcca gcatatcgcc aactggttac gtcacggtat
    19441 tctgaccaaa gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca
    19501 aaacgctggc gatccggctt atcgtccgat ggcggggaat ttcgctaact cgtgtgcttt
    19561 taaagctgcc agcgatttaa tcttcctcgg cgtgaaacag ccaaacggct ataccgaacc
    19621 gttattacac gcctggcgtt tacgcgaaaa agaaagtcat taatagcgtc aaaaaagccc
    19681 cggcaagttt tattgtcggg gctatgatag taactgcggt agtacattta tcccggaaat
    19741 aaccggagcg attatcaggg tttaacaata ttaaataccc gtagcgcatt actagcgggc
    19801 ctaaaacaac aatatggaca tcttaacgat actctgcgtc tgcgtgtgcg gaacagaaac
    19861 ccttacccga tatacttgtt atgccagagg tagcaattgc gttagcggcg tactgttgct
    19921 gtaaagtata acgcctatat tttacagttc agtcctcttt tgcttattac aaccttaagt
    19981 atatcaagca tataaagata ataagagact gaacaatatg gttacctgga cccaaatgta
    20041 tatgccgatg ggaggactgg ggctatccgc tctggtcgcc ctgatcccga taatattctt
    20101 cttcgttgca ctcgcggtat tacgtctgaa aggacatgtc gctggagcaa taacccttat
    20161 attatctatc ctgattgcaa tattcgcctt taaaatgccg attgatatgg catttgctgc
    20221 tgcgggctat ggctttattt atggattatg gccaatagcg tggattattg tcgcggcggt
    20281 gttcctgtat aaattaaccg ttgccagcgg gcagttcgat attatccgca gctcggttat
    20341 ctccatcacc gacgatcagc gtttgcaggt gttactgatt ggtttctcct ttggtgcgtt
    20401 gctggaagga gcggctggct ttggtgcgcc ggtggcgatt accggtgcgc tgctggtggg
    20461 cctgggcttc aaaccgttat acgcggcggg gctgtgtctg attgccaata ctgcgccggt
    20521 ggcgtttggt gcgttgggcg tgccgattct ggtcgccggt caggtaacgg gaatcgatcc
    20581 gttccacatt ggcgcaatgg cgggacgtca gttaccgttc ctgtcggttc ttgtgccgtt
    20641 ctggctggta gcaatgatgg acggctggaa aggggtgaaa gagacgtggc cagcggcgct
    20701 ggttgctggg ggaagcttcg ctgtcactca gttctttacc tctaactata ttggtccgga
    20761 actgccggat attacttcgg cgctggtgag tatcgtctca ctcgctttat tccttaaagt
    20821 ctggcggccg aaaaataccg aaacggcaat cagcatggga caatccgcag gtgcgatggt
    20881 ggtaaataag ccatcttctg gcggtcccgt gccttcagaa tatagtctgg ggcaaatcat
    20941 tcgagcgtgg tcaccgtttt taatcttaac ggtgctggtc accatctgga ccatgaagcc
    21001 gtttaaagcg ttatttgctc cgggcggcgc gttttattca ctggtgatta atttccagat
    21061 ccctcatttg catcaacaag tgttgaaagc ggcacccatt gtcgcccaac caacgccaat
    21121 ggatgcggtg tttaaattcg accccctctc ggctggcggc accgctattt ttattgcggc
    21181 gattatctct atcttcatcc tcggtgtggg gatcaagaaa ggtattggcg tctttgccga
    21241 aacgctaatt agcttgaagt ggccgatact gtcgattggc atggtgctgg cgttcgcctt
    21301 cgtcaccaac tattctggca tgtccaccac gctggcgctg gtactggcag gtacaggcgt
    21361 gatgttcccg ttcttctcac cgtttctcgg ctggctgggc gtattcctta ccggctcgga
    21421 cacctcctct aacgccctgt ttggttcact gcaatcgacc acggcgcagc aaatcaacgt
    21481 ctctgacacc ctgctggtgg cagcaaacac cagcggcggc gtaactggca agatgatctc
    21541 cccgcaatct atcgccgtgg cctgcgccgc gacgggcatg gtgggccgag aatctgaact
    21601 gttccgctac accgtgaagc acagtctgat ttttgccagc gttatcggca ttatcaccct
    21661 gctgcaggcg tatgtgttta ccgggatgtt agtctcgtaa atcaataaag ccggatgatc
    21721 gttaatatca tccggcttca tttacattat tctgtcttat taattgaaaa tcatttctcg
    21781 ctttattatc catgcagcaa taatgttttg cgttcagata acctgctaaa taattaagag
    21841 cgtgagaaca tctatttttt tatcacgcaa aacaagtctg tattaattat gttcgcttga
    21901 tgagcaaaca ccgttctatc tgttgctttt gcaatcttaa tcgttaaaac taccaacaaa
    21961 tttatatagt tacgttgtga aatgttgtta attttttgtt tgttgaaatt tcgtaaaaat
    22021 agccagatag acatcgtata ggggttattc ttataaattt cacctgtttt ctatca
//
LOCUS       DBBGLQ010000071        21769 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.071, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000071 DBBGLQ010000000
VERSION     DBBGLQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.071"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..164
                     /locus_tag="ACIYSB_003609"
     CDS             <1..164
                     /locus_tag="ACIYSB_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5430574.1"
                     /translation="RLVREKERYEITSVSRTQAWKLEREGKFPPRKSIGKKSCGWLFS
                     DLLWWIQTR"
     gene            321..522
                     /locus_tag="ACIYSB_003610"
                     /pseudo
     CDS             321..522
                     /locus_tag="ACIYSB_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335713.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
     gene            738..2171
                     /locus_tag="ACIYSB_003611"
     CDS             738..2171
                     /locus_tag="ACIYSB_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGC5430575.1"
                     /translation="MSVLALEKPYPTYALPPLVRNAIIETQKNTQAPLALVATSALTA
                     MSVSSQNQADVCRPGNLCGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLHKEIRAGKDCKATESELKRHLNKCPVPPQIHRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQYLFEQRINELIDESLAMRGERRCLHFSPQAARIWMDYYNDVESKMAVLGP
                     LRDFREYAAKNAEYMARLAGLIHHFSGEEGDISPYTAEMARELAIWYGNEYIRLSSPL
                     TFENSAQTVTMRLIPEELELFSWIKSYCIEKGIPCMKKNDILQRGPNRFRKKDKLNWL
                     LDLLHEQNRVVPVIEGKTWYVAPNFDL"
     gene            3030..3593
                     /locus_tag="ACIYSB_003612"
     CDS             3030..3593
                     /locus_tag="ACIYSB_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGC5430576.1"
                     /translation="MYNANPNYEMNFMILKDVNEHMEGLFQRFSKLLPFRIDFAYKKG
                     TPSFGYSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRYKKS
                     YRLSRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKELGIIWWHSRLPEKSNRGRPRHN"
     gene            complement(3919..5254)
                     /gene="ltrA"
                     /locus_tag="ACIYSB_003613"
                     /pseudo
     CDS             complement(3919..5254)
                     /gene="ltrA"
                     /locus_tag="ACIYSB_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(5748..5867)
                     /locus_tag="ACIYSB_003614"
                     /pseudo
     CDS             complement(5748..5867)
                     /locus_tag="ACIYSB_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(5946..6176)
                     /locus_tag="ACIYSB_003615"
                     /pseudo
     CDS             complement(5946..6176)
                     /locus_tag="ACIYSB_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            complement(6320..7429)
                     /locus_tag="ACIYSB_003616"
     CDS             complement(6320..7429)
                     /locus_tag="ACIYSB_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5430577.1"
                     /translation="MSNIRLRNVTKRFGSTVTLHQVNLDIEDGEFAVFVGPSGCGKST
                     LLRMIAGLEEVSEGEVLIGDEVMNDVVPARRGVAMVFQSYALYPHMTVAENMGYGLKV
                     NKVPKEEIRRQVEMVAKTLQLSHLLDRKPKQLSGGQRQRVAIGRAIVRNPRVFMFDEP
                     LSNLDAELRVDMRLHIARLHQELKTTMVYVTHDQVEAMTLADKIVVMNHGKVEQMGSP
                     MALYYNPVNKFVAGFIGSPKMNFLPAVVSDWQPECLTVTLAQDHQLTLNIATQPLKPG
                     AAVTLGIRPEHLTPEVTTGTVVEFQCEVVERLGNNTYLFGQCYGHDNVKVLLPGDVHF
                     RPWQKINLAFDERFCMVFDENDLRISADITAPDAH"
     gene            7786..9018
                     /locus_tag="ACIYSB_003617"
     CDS             7786..9018
                     /locus_tag="ACIYSB_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin ABC transporter substrate-binding
                     protein"
                     /protein_id="HGC5430578.1"
                     /translation="MKKNTLAALILTTLAVGQLTSLQAHAAGQLNVWEDIKKSAGIKT
                     AVSDFEKQYNVKVNLQEMPYAQQLEKLRLDGPAGIGPDVLVIPNDQLGGAVVQGLLSP
                     LSVDQAKQDAFTPASINAFRMDNALYGIPKAVETLVLIYNKDLIDKPLDSLQAWLDYS
                     KTQREQNKYGLLAKFDQIYYSWGAIGPMGGYIFAKNDSSGFNPQQVGLNTPGAVEAVT
                     FLKKFYAEKVFPAGILGDNGLNAIDSLFTEKKAAAVINGPWAFQPYEAAGINYGVAPL
                     PTLPDSKPMSSFLGVKGYVVSTWSKDKAMAQQFIEFINQPQYVKDRYVATGEIPPLKA
                     MIDDPLIKNDQKASAVAIQSARAVAMPGIPEMGEVWGPANAALELSLTGKQAPQAALD
                     NAVKQITMQIEAMQASNQ"
     gene            9081..10388
                     /locus_tag="ACIYSB_003618"
     CDS             9081..10388
                     /locus_tag="ACIYSB_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGC5430579.1"
                     /translation="MSIHSSENFGDARGPGRHAWCGLLLAIVPGFGQFYHRQWLKGLV
                     FLALLSSFLGIFYDFLREGLWGLYTLGEEVPRDNSIFLLAEGIISVLIVAFGVLIYFL
                     SLRDAWLNGKKRDEGSVLNSVRKQYQMLLSDGFPYLMITPGFILLVFVVIFPILFGFA
                     IAFTNYNLYHTPPAKLVDWVGLKNFVNIFTLSIWRSTFLDVLQWTVVWTLLATTLQCT
                     VGVLLAILVNQKDLRFKPLIRTIFILPWAVPGFVTILVFAGMFNDSFGVINNAILSFF
                     GISPKAWLTDPFWTKTALIMMQTWLGFPFVFAMTTGVLQAIPDDLYEAATMDGASAFT
                     RLRTITLPLVLYAIAPIIITQYTFNFNNFNIIYLFNNGGPAVVGSNAGGTDILVSWIY
                     KLTMSSSQYAIAATITILLSIFVVGLALWQFRATKSFKNDDMA"
     gene            10399..11250
                     /locus_tag="ACIYSB_003619"
     CDS             10399..11250
                     /locus_tag="ACIYSB_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGC5430580.1"
                     /translation="MAQSPSIKREKWIRLSLTWLVVILVSVVIIYPLVWTVGASLNAG
                     NSLLSTSIIPENVSFQHYADLFNGNVNYLTWYWNSMKISFLTMVLTLISVSFTAYAFS
                     RFRFKGRQNGLMLFLLLQMIPQFSALIAIFVLSQLLGLINSHLALVLIYVGGMIPMNT
                     WLMKGYLDAIPKDLDESARMDGASSFRIFIEIIMPLSRPILAVVALFSFTGPLGDFIL
                     SSTILRTPDKYTLPIGLYNLVAQKMGASYTTYAAGAVLIAVPVAILYLALQKYFVSGL
                     TSGSTKG"
     gene            11255..12457
                     /locus_tag="ACIYSB_003620"
     CDS             11255..12457
                     /locus_tag="ACIYSB_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HGC5430581.1"
                     /translation="MKRFTPAWLAVCLACSFSTSSLAADELETRAFQGMPADFIKGAD
                     ISTLLDAEKHGATFYDQNNQRKDPIAILKENGVNYVRLRLWVDPQSASGEDYGGGNND
                     LATTLALAKRAKVQGMKLLLDFHYSDFWTDPGKQFKPKAWEKMDYPQLKTAIHDYTRD
                     TIARFKQAGVLPDMVQIGNEINGGILWPEGKSWGQGGGEFDRLAGLLNSAIDGLKENL
                     RNGEHVKIMLHLAEGTKNDTFRWWFDEIDKRHVPYDVIGLSMYTYWNGPISALKANMD
                     DISKRYNKDVIVVEAAYAYTLANCDNAENSFQAKEEKDGGYPATVQGQYNYIHDLMQA
                     VVNVPDQRGKGIFYWEPTWIAVPGNTWATPAGMKYIHDEWKEGNARENQALFDCQGKV
                     LPSVKVFN"
     gene            12489..14546
                     /locus_tag="ACIYSB_003621"
     CDS             12489..14546
                     /locus_tag="ACIYSB_003621"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGC5430582.1"
                     /translation="MNKFAPLHPKVNTLLHGADYNPEQWENDPDIIDKDIAMMQQAKC
                     NVMSVGIFSWAKLEPREGVFDFAWLDTILDKLYAAGIHVFLATPSGARPAWMSQRYPQ
                     VLRVGRDRVPALHGGRHNHCMSSPVYREKTLKINSLLAERYSSHPAVLGWHISNEYGG
                     ECHCDLCQNRFRDWLKARYQTLENLNQAWWSTFWSHTYTDWSQIESPAPQGEMSIHGL
                     NLDWHRFNTAQVTDFCRHEIAPLKAANSALPVTTNFMEYFYDYDYWQLAEVLDFISWD
                     SYPMWHRDKDETALACYTAMYHDMMRSLKGGKPFVLMESTPGATNWQPTSKLKKPGMH
                     ILSSLQAVAHGADSVQYFQWRKSRGSVEKFHGAVVDHVGHIDTRIGREVCQLGEILSK
                     LPEVRGCRTEAKVAIIFDQQNRWALDDAQGPRNLGMEYEKTVNEHYRPFWEQGIAVDV
                     IDADVDLTPYQLVIAPMLYMVRDGFAGRAEAFVANGGHLVTTYWTGIVNESDLCYLGG
                     FPGPLRNLLGIWAEEIDCLNDGEFNLVQGLAGNQCGLQGPYQVRHLCELIHTESAQAL
                     ATYRDDFYAGRPAVTVNEFGKGKAWHVASRNDLAFQRDFFTTLSKELALPRAIATELP
                     PGVVATARTDGDNSFIFLQNYSAQNHTLTLPQGYWDCLTDAAVSAPLTLSAWDCRILR
                     RHA"
     gene            14610..14924
                     /locus_tag="ACIYSB_003622"
     CDS             14610..14924
                     /locus_tag="ACIYSB_003622"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HGC5430583.1"
                     /translation="MVSLKSFLHYFSPARPAQPLSEAEKQQIEALIQAFGGEANITQV
                     DACVTRLRVTVRHLADVDTDALQQQGALGVIILCQQVHAIFGKQSDALRQLLDEHFAA
                     RK"
     gene            complement(15061..16332)
                     /locus_tag="ACIYSB_003623"
     CDS             complement(15061..16332)
                     /locus_tag="ACIYSB_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HGC5430584.1"
                     /translation="MNTTLRALSVALAAALIAPSAFAASAAIPTIDFHGYMRAGVGVS
                     SDGSEVEWQKNKLGRLGNESDTYGELELGSEVYKKDDVSFYLDSMVSMVSDGSNDNET
                     TLNDDAQFGLRQLNLQIKGLIPGDPNAVIWGGKRYYQRHDLHIIDTKYWNISGSGAGV
                     ENYTLGPGAVSLAWIRGDANDVDYRVDGDSNVNINYIDLRYAGWKPWAGSWTEFGIDY
                     AMPNTTKKQDSYGGLYDADNGVMLTGEISQDMLGGYNKTVLQYANKGLAQNMVSQGGG
                     WYDMWNYVNDATGYRVINTGLIPITEKFSINHVLTWGSADDITDYTDKTRMLSLVARG
                     QYQFTDYVRLIGEVGGFYQKDSYNNGTSYKQAGEKYTIALGLADGPDFMSRPELRIFA
                     SYLNDSEDGKPFEDQTANNTWNFGVQVEAWW"
     gene            16603..17673
                     /locus_tag="ACIYSB_003624"
     CDS             16603..17673
                     /locus_tag="ACIYSB_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGC5430585.1"
                     /translation="MKSKSATLEDVARHAGVSYQTVSRVLNKSANVSEATRRKVEKSI
                     EALRYVPNRLAQQLVGKESQTVGLVTISLAWHAPSQVATAVKRYANLEGYQVLISMID
                     ESVNQSIQDSINELKSQRVDKVIINVPLETEQAQKIAADNDDIVCLFLDVDPYSSVFN
                     VSFNPADGTRASVKYLYEMGHREIALLAGPDNSVSAKLRLKSWLETLQGYGLKPVTVL
                     HGNWDAQSGYAGALQMLRETPNFSAVLVGNDQMALGVLSAFHQHQVAVPGEKSVIGYD
                     DTYESSFFYPSLSTVSLDLDLQGKEAVRRILASTSGAPHTSSILPARLVIRHSSGARI
                     EQGKDLQAIAEQLRAIAHTLAP"
     gene            18006..18519
                     /locus_tag="ACIYSB_003625"
                     /pseudo
     CDS             18006..18519
                     /locus_tag="ACIYSB_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(18517..19071)
                     /locus_tag="ACIYSB_003626"
                     /pseudo
     CDS             complement(18517..19071)
                     /locus_tag="ACIYSB_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            19500..20372
                     /locus_tag="ACIYSB_003627"
     CDS             19500..20372
                     /locus_tag="ACIYSB_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGC5430586.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDVAGIQPSPAQARNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            20666..21268
                     /locus_tag="ACIYSB_003628"
                     /pseudo
     CDS             20666..21268
                     /locus_tag="ACIYSB_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529398.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
     gene            complement(21300..>21769)
                     /locus_tag="ACIYSB_003629"
     CDS             complement(21300..>21769)
                     /locus_tag="ACIYSB_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5430587.1"
                     /translation="SELYAIEDEIRGLPESERLAVRQQRSKVLLTSLHEWIVEKNGTL
                     SKKSRLGEAFSYVLNQWDALCYYSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHG
                     GERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
BASE COUNT         5220 a         5423 c         5650 g         5476 t
ORIGIN      
        1 acagactggt acgtgaaaag gaacgatatg agatcacgtc agtttctcgt acacaagcgt
       61 ggaaacttga acgggaggga aaatttccac cacgtaagtc tatcggaaag aaatcctgtg
      121 gatggttatt cagcgacctt ctctggtgga tacagaccag atgaactgtt tgaatatttc
      181 agaatatgca gtctgtatag cagctattgt cgctattgta gcgggaatag ctgctatacc
      241 cgaaactcat tatgaaaaag ggagcattta tgtcttacat caaatcggat actcgatatc
      301 cagtatatac gtaaccatca atgcattgga aatgcaataa ttgaagctca acaaaatgaa
      361 aaagcatctc tggctatgac gataacatca gcattaacag cgatatcaat tgtctgtcag
      421 aatcaggctg aggggggcag accaggaatc actaatattt actctatggt tcttgatgat
      481 cccggagaaa ggaaaacgac catggataag atgttgatga aataattttc aggaggtatt
      541 aagaatgaga aatatgaatg atatttcgtg gtgaataaca tggtctggac ttaattgatt
      601 ttatcggttt attaaacatc agaactacag atactctgta tgttctcaga acattaggcc
      661 ttaatagcag ctattttcgc tattatagcg ggagttgctg ctactttact gaccttttaa
      721 taaaaataga ggtgtttatg tccgtacttg cactggaaaa accttacccg acatatgcac
      781 taccaccgct ggtgagaaat gcaataattg aaactcaaaa aaatacacag gcacccctgg
      841 ctctggtggc aacatccgca ttaacagcga tgtcagtttc cagccagaat caggctgatg
      901 tatgcaggcc aggaaattta tgtgggcctg ttaatcttta ctctctgatt ctggctgatt
      961 ccggtgaaag gaaaacgacc gtggataaag tgtttatgaa agcattttat ctcagggatg
     1021 aagccctggc ggaagaatac gcgaaactgg ttgagaatta cagtacagaa aaggaaatat
     1081 gggagcaaaa gcaaaaagca ctggagtcaa aattgcataa agaaattcgc gccggtaaag
     1141 attgtaaggc aacagagtca gagcttaaaa gacatctgaa taaatgtcct gttccgccgc
     1201 agatacaccg gacaattttc aatgagacaa cgatagaggg aatgttaaaa tattattccg
     1261 acagcaatcg ctcttttgct cttgtatcca gtgaaggagg aataattttt gatggtcggg
     1321 caatgagtaa acttggaata cttaactctt tgtgggatgg tggttctctt ttcatcgaca
     1381 ggaaatcatc tcccggaatt attttgaagg atccaagact gacggtatcg gtgatgattc
     1441 agcctgatgt ttaccagaaa ggtttttgta cacgaaaaaa agaacttgtg aaaacgtcag
     1501 ggcatcatgc aaggtttttg atgtgtcaac caacatcaac gcaggggaca aggataataa
     1561 ccggagataa ttattcatca cagtatcagt atttgtttga acagaggata aatgaactca
     1621 ttgatgaaag cctggctatg aggggagagc ggcgttgcct tcacttttct cctcaggcgg
     1681 ccagaatctg gatggactat tacaacgatg tggaatcgaa gatggcggtt ctggggcctt
     1741 taagagactt cagagaatat gctgctaaaa atgcagagta tatggcaaga ctggcaggac
     1801 ttattcacca ttttagcggt gaggaaggtg acatttcccc ctacactgct gaaatggcga
     1861 gagaattagc aatatggtat ggtaatgagt atattcggtt atccagtccg ctaacgtttg
     1921 aaaactctgc ccagaccgta actatgcgac tcattccaga ggagcttgaa ctttttagct
     1981 ggataaaaag ctattgcatt gagaagggga taccctgtat gaaaaaaaat gacattttac
     2041 agcgtggtcc taaccgtttc cgtaaaaaag ataaactcaa ctggttactg gatttactgc
     2101 atgaacaaaa cagagttgta ccggttattg agggaaaaac gtggtatgtc gcacctaact
     2161 ttgacctctg aacgacttaa tcgttgagta acatatagtc tcctctgcta cttgcggagg
     2221 agactaatgc ctttaccatg tcagcgttgt tgtattacag aactctggta caggtcagtc
     2281 agcaaatata actggtataa ccatgattta ctattggata gagtgttgtt caggatattg
     2341 ctggctttag tcttggatga ggagaccccc tcattatttt tgtgttcaat aaataattta
     2401 ggttaattag tattattaat gggagttata ttatctgtta tgttgcctta tctcttttac
     2461 atggtagtgt cactcctctt tacgctaacc attctgttta gtctgtaggt gtgtacttga
     2521 caccagatgc gtgccagaac tctattagaa atatattatc tttatttcgt atgttatagc
     2581 tccgttacag tttataggta tgggaaaata taatattttc tgtgtgtaat aatcttcttg
     2641 tggtgaggtt aaatatctgt agagtctctc tgcagagcct ctgtttttat ttaatggtgc
     2701 ggaagtgtca atagtggtgt tgtctgtggc tacccgatat tgacttttgt gaagatcaga
     2761 ttacttcctg taagtgattg aatgatgctt tcttaaactc aattctatga ggtgtgtttt
     2821 taccattgta attcgtactg ttcctcagtt taagagagtc tctgaatcta ttacctcatg
     2881 acagcaatga ggtaatgcaa aacagtttta tcaggttgtc tgtgctgctg gtttacttcg
     2941 tttattaatg ccgtggtaca gcgtttctgt actctcagta tttacgtaat ttcctcttcc
     3001 tgtgagtttt ataaagtaag gactataaaa tgtataatgc taatcccaat tacgagatga
     3061 atttcatgat tctgaaagat gtgaatgaac acatggaagg tctgtttcaa cgtttttcta
     3121 aactgttgcc tttcagaatt gattttgcct acaagaaagg cacgcccagt ttcggctatt
     3181 cctgtaagca cagtatgtgt atggaaatat accgcttact tagtgaaacc caaacgatgc
     3241 tggcaggata ttactgggta atggaatata caccagacaa aggactccat attcatttta
     3301 tcggctacct ggatgggcaa cgttacaaga aatcttatcg gttatccagg caactggggg
     3361 atatctggag gcgaatcacg gaaggggagg ggtactttca cctgtgccgg gctaaagaca
     3421 aatacccggt gcgtatcgat cacgtgattc actatagtga taagtccgcg gtagacgact
     3481 tacgttacgc cctcagctac ctggccaaac aggatcaaaa agaacttggt attatttggt
     3541 ggcatagcag acttcccgaa aaaagtaacc ggggccgacc acgacataac tgaattatgg
     3601 gggcttatgc ccgaactatc gtaatttatt gcgtggattt gtcgttaatg cgtagctttg
     3661 tcctcagcgc aggggacggg actatgacac tttgaacgtc atcttgggtg tagtgacttt
     3721 taacgtcaga tgatgcataa gggcctttcg ctaagaggct ctgtttacac aattaaataa
     3781 attgtcaacg ccacgatttt tgaccgttag gggatgggtg agtagacccg tcggtcaccc
     3841 gggcgggccc ctccgcagaa ccggacgtgc ggaactaccg catccgactc ccgacagatc
     3901 agtgttccca cacacatttc agcaagaagc gaacagtgaa cgggttttcc atctcgatgg
     3961 aaaacccagc attttcagga ttatattcag cgttttccac gtcattcggc gacgcacacc
     4021 tttacgatta aaccatctgt agattattcg cgtactttgg aatataaact gactcacacg
     4081 cctctggtta tcagatatac agtgataatt tatccagcct ctgattactc tgatgatggt
     4141 attcagaaca agccttttat tgggggcatt cagatttctc cagaggaaat ctctgaggcc
     4201 ctttagtttg gctgcgaaac gatctttccg gctgcttaac tttaaacgcc agtgtccttt
     4261 tcgtgtttta ccccagtagc atgtgaatcc cagaaaatta aacgtaggca ggcgttttcc
     4321 cgactggtta gctctcattg ctgctatatg acccgcggga attaattgtg atttatcaca
     4381 atgcaactcc agaccatatt tattcagcct tttcggtagc ctcctaaaga agcgttttgc
     4441 ttcactaaag aactcaaagg taaataccat atcatcagca tacctcacca tctcagcccg
     4501 cccatggatg tgtgaacgcg tgatttgagc aaaccattcg tctattacat gatgcaggta
     4561 gatattagcg agtattggcg acagggccga accctgcggg caaccctgtt cattctctga
     4621 tatctctttc cccctgataa caggtgccgt tattgagacc tcgaccagcc tgagaaaacg
     4681 acgatctgat gtcttcttcc gcagtaaatt catcaattca gtatgtggaa ttgtgttgaa
     4741 atactttcgg atgtcgattt ccaccaccgc gccattccag ttgcggaacg tttgctgttg
     4801 caaagccttc aaagcagcat gacaatttaa tcccggtcgg aaaccgtatg agcagggcag
     4861 aaacagtggc tcatagacac tgctgagaat atcgctgaca gcaagttgca ccagtttatc
     4921 ctcgatgcac gagagagcca gcggccgttt actgccgtcc tcttttggta tctccgtgat
     4981 tcttgccgct tgcgggtgat acgtccctct gcgtatacgc acgatgagag aagtaatgtt
     5041 ctcatcaagg cgttcactgt aagcggcttt agtccatacc atcaatacct gtagccttat
     5101 tcccgtcgag tagcaggaat tgtcctttca gcatgtcaac gttcaccaga tgccccagat
     5161 tactgaacat cttatgtttg ttggctgctg atttctcgcc tatacgctca agtttcgtta
     5221 accatgatag tccgtcgttg ctgtgtacgg tcattgtttc tctctcacgt tcgatctgtc
     5281 cgtcagccct tcgatccacg atcattactc gcttcatcac tactatggct gactccgact
     5341 tcctgatacc cgtttctgtg gtcttgtgtt ttcgcacttg tacctgagat actcgttccc
     5401 ttgagaggca tcaggagctc ctgggttcca cactgttctc tgcaaacacg ccgacgcctg
     5461 cggctccggt gtgtgcggtt gcatggatgc tcgccagtgt gaccatgcaa ccgctgttgc
     5521 ctgcaagctg aacgaaactt tcggcacaca cgattaaaat gtatttcgga tctgtcacgt
     5581 tcaccagttg gtttcggctc gggtgcttcg ctgtctacgc ttcatctcct ttgttaccgc
     5641 cggagatgca agactcgcta cgtagaggtc tggctggtcc tttctacgga gggacttaca
     5701 ccctcaagat cagtgcagct ttgcccagcg caccgttcat ctcattccca gaagtgcagt
     5761 gctttcattc ttaatttggc gatctcaggc ataacagagt gtttcatcgc atagatggct
     5821 gcacggtgat aacccaaata cccgacgccg acatgttgca ttaacatagc aatctccctt
     5881 tacttatccg gaggcagttg caatatctct gtgaacttac acacatggtg gtaataatat
     5941 ttttaaatca tatggctatc atgatgaagc aattcattac caagcagacg atccactcgt
     6001 ttaattttgt ttttgacttg tgcgttgccg ggcaaataac gtctaatact ggtcagcatg
     6061 agaaatgtgc ccttaatgag cgcagtggtg gcatccagta gagaattttg tagatatttg
     6121 tgaaacggcg cgagagcatc acggaaaaaa ttctgacata catgacgagt aggcatagag
     6181 ttgatctccc tgaattgata gcacaaatca gtaaatcaca aaaccctatg cctgcctttg
     6241 tttactcgcg catttctaga gattcctcat agctaggata gactttaaca tacgcagcgt
     6301 agatgacaaa aatgttggct tagtgcgcgt ccggcgccgt gatgtcggcg ctgatgcgca
     6361 gatcgttctc atcaaagacc atgcagaagc gctcatcgaa ggccagatta atcttctgcc
     6421 acgggcggaa atggacgtcg ccgggcagca aaaccttgac gttatcgtgg ccgtagcact
     6481 ggccaaacag gtaggtattg ttacccagac gttccaccac ttcacattga aactccacca
     6541 ccgtaccagt agtgacctcc ggagtgaggt gttctggccg gatccctaac gttaccgcag
     6601 cgccgggctt cagcggttgg gtggcgatat tcagggtcag ctgatggtcc tgcgcgaggg
     6661 tgaccgttaa gcattccggc tgccagtcgc tgacgacggc cggcaaaaag ttcatcttcg
     6721 gcgagccgat aaagccggcg acgaatttgt tgaccggatt gtaatacagg gccatcggcg
     6781 aacccatttg ttcgactttc ccgtggttca tcaccacgat tttatcggcc agggtcatcg
     6841 cttccacctg atcatgtgtg acatacacca tggtggtttt cagttcctgg tgcagacggg
     6901 caatatgcag gcgcatatcg acgcgcagct ccgcatcaag gttagagagc ggctcatcga
     6961 acataaacac ccggggatta cgcacgatcg cgcggccaat ggccacgcgc tgccgctgtc
     7021 ctccggagag ctgcttcggt ttacggtcca gcaggtgcga gagctgcagg gttttggcca
     7081 ccatttccac ctggcggcgg atctcctcct tcggcacttt attcaccttc agcccgtagc
     7141 ccatattctc cgccacggtc atatgcggat agagcgcata ggactggaag accattgcca
     7201 cgccacggcg ggcgggcacc acatcattca tcacctcatc gccgatcagc acttctcctt
     7261 cgctgacctc ttccagcccg gcaatcatgc gcagcagcgt cgattttcca caaccggaag
     7321 ggccgacgaa aacggcaaac tcgccatctt caatatcgag attgacctgg tgcagcgtca
     7381 ccgtgctgcc gaaccttttg gtgacattcc tcagtcgtat attggacatc cacattcccc
     7441 ggagtgagta gctgttattt gccgttggtt tgtcactatg ttgagtatga tttgtattac
     7501 gtataacaaa tcggtgtgac gatgttcaac agcagtaaag tcatggtcta actataactt
     7561 ccacggattt tgcgccatac agggacgttt catttctgca aggatgatca cacaactggc
     7621 agattatggt agttttatca acgatcaaag cgtagcgtat aacaaaagcg aatcagaaca
     7681 tgttttcaat gtaatacggc agtttgaaat gatttatttt tctaactggc agacgtaagt
     7741 cacataaaaa ccattaaatt gttatacctt cacatgaggt tgataatgaa aaagaacacg
     7801 cttgctgcac taatccttac caccctggcg gtggggcaac tcaccagcct gcaggcgcac
     7861 gccgccgggc agctcaacgt ctgggaagac attaagaaat ccgcgggtat taaaaccgcg
     7921 gttagcgatt ttgaaaagca gtacaacgtg aaggtcaatc tgcaggagat gccttatgcc
     7981 cagcagctgg aaaaactgcg tctcgacggg ccggcgggca ttgggccgga cgtgctggtg
     8041 atcccgaatg accagctcgg cggcgccgtg gttcagggac ttctctcgcc gctcagcgtc
     8101 gatcaggcga aacaggatgc ctttacgcca gcctccatca atgccttccg catggacaac
     8161 gcgctgtatg ggatcccgaa ggcggtggaa actctggtgc ttatctacaa caaggacctg
     8221 atcgacaagc cgctggacag cctgcaggcc tggctggact actcgaaaac ccagcgcgag
     8281 cagaacaaat acggcctgct cgccaagttt gaccagatct actacagctg gggggcgatt
     8341 ggcccgatgg gcggctatat ctttgctaaa aacgacagca gcggctttaa cccgcagcag
     8401 gtgggcctca atacccccgg cgcagtggaa gccgtcacct tcctgaaaaa attctatgcc
     8461 gagaaggtct ttccggcggg gatcctcggc gataacgggc tgaatgccat cgattcgctg
     8521 tttaccgaga aaaaagcggc ggcggtgatc aatgggccct gggccttcca gccatatgaa
     8581 gccgctggca ttaactatgg cgtggcgccg ctgccgaccc taccggacag taagccgatg
     8641 agctccttcc tgggcgtcaa aggttatgtg gtatcgacct ggagtaaaga caaagcgatg
     8701 gcgcaacagt ttatcgagtt catcaaccag ccgcagtatg tgaaagaccg ctatgtcgcc
     8761 accggcgaaa ttccgccgct gaaggcgatg attgacgatc cgctgattaa aaacgaccaa
     8821 aaggccagcg ccgtcgctat ccagtcggcg cgggcggtgg cgatgccggg cattccggag
     8881 atgggggaag tgtgggggcc ggccaatgcc gcgcttgaac tgagcctcac cggcaagcag
     8941 gcgccgcagg ccgcgctcga taatgcggtg aaacagatca cgatgcagat cgaagcaatg
     9001 caggccagca atcagtaatt caccgttacg tgaagtcacc gagcgggagg gggacctccc
     9061 gtttgtagaa ggagttgagt gtgagcatcc attccagcga aaattttggc gacgcgcgcg
     9121 ggcccgggcg gcatgcgtgg tgcggcctgc tacttgcaat cgtgccgggt ttcggtcagt
     9181 tttatcatcg ccagtggctg aaagggctgg tcttcctggc gctgctgagc agctttctgg
     9241 gtatttttta tgatttcctg cgcgaggggc tgtgggggct atataccctg ggcgaggagg
     9301 taccgcgcga taactctatc ttcctgttag ccgaggggat tatcagcgtg ctgatcgtcg
     9361 cctttggcgt gctgatctat ttcctctcgc tgcgtgatgc gtggctcaac ggtaaaaaac
     9421 gtgatgaggg gagcgttctc aacagcgtgc gtaagcagta ccagatgctg ctaagcgacg
     9481 gctttccgta cctgatgatc accccgggtt ttattttgct ggtctttgtg gtgatctttc
     9541 cgattctttt tggttttgcc atcgctttca ccaactacaa cctgtaccac acgccgccag
     9601 cgaagctggt tgactgggtg gggctgaaaa acttcgtcaa tattttcacc ctctccatct
     9661 ggcgctccac cttcctggat gtgctgcagt ggaccgtggt atggacgctg ctggcgacca
     9721 ctctgcagtg tacggtgggc gtgttgttgg cgattctggt caaccaaaaa gatctgcgct
     9781 ttaaaccgct gatccgcacc atctttattt tgccgtgggc ggtgccgggg tttgtcacca
     9841 tcctcgtctt tgccgggatg ttcaacgact cctttggggt catcaacaac gcgatccttt
     9901 cattcttcgg catcagtccg aaggcatggc tcaccgatcc gttctggacc aaaacggcgc
     9961 tgattatgat gcagacctgg cttggcttcc cgtttgtgtt tgccatgacc accggcgtgc
    10021 tgcaggcgat cccggacgat ctgtatgaag ccgcaacgat ggacggcgcc agcgcgttta
    10081 cccgtctgcg caccatcact ctgccgctgg tgctgtacgc gatcgcgccg ataatcatca
    10141 cccagtacac cttcaacttt aataatttta acatcattta cctgttcaac aacggcggcc
    10201 ctgcggtggt gggttctaac gccggcggga cggatattct ggtgtcgtgg atctataagc
    10261 tgacgatgtc ctcgtcgcag tacgccatcg cggcgaccat cacgatcctg ctgtcgatct
    10321 ttgtcgtcgg cctggcgctg tggcagttcc gcgccaccaa atccttcaaa aatgacgaca
    10381 tggcgtaagg gaagcaacat ggctcaatca cccagtatta aacgcgaaaa gtggatccga
    10441 ctctctctca cctggctggt ggtcatcctg gtctcggtgg tgattatcta tccgctggtc
    10501 tggacggtcg gggcatcgct gaacgccggc aatagcctgc tcagtacctc aatcattccg
    10561 gaaaatgtgt cgttccagca ctatgccgat ctgttcaatg gcaatgtgaa ttacctcacc
    10621 tggtactgga actcgatgaa aatcagcttc ctgaccatgg tactgaccct gattagcgtt
    10681 agctttaccg cttacgcctt ctcccgcttc cgctttaaag ggcggcagaa cgggctgatg
    10741 ctgttcctgc tgctgcagat gatcccgcag ttttctgcgc tgattgcgat ctttgtcctg
    10801 tcgcaactgc ttggcctgat taacagccat ctggcgctgg tgctgatcta cgtcggaggg
    10861 atgatcccga tgaatacctg gctgatgaaa gggtatctcg atgcgatccc taaagacctg
    10921 gatgagtccg ctcgcatgga cggtgccagc agctttcgca tctttatcga gatcatcatg
    10981 ccgttgtcga ggccgatcct cgcggtggtg gcgctgttct cgttcactgg gccgctgggg
    11041 gatttcatcc tctccagtac catcctgcgt accccggata aatacacgct gcctatcggt
    11101 ctgtataacc tggtggcgca aaaaatgggt gccagctaca ccacctacgc ggcgggggcg
    11161 gtgctgatcg ccgtaccggt ggcgatcctc tacctggcgt tacaaaaata cttcgtctcc
    11221 ggcctgacct cgggcagcac taaaggataa gcacatgaaa agattcacac ccgcatggct
    11281 tgcggtttgt ctggcatgca gtttttccac ctcttctctg gccgcggacg agcttgaaac
    11341 ccgcgccttt caaggcatgc cggcggactt tatcaaaggc gcggatatct ccacgttgct
    11401 ggacgccgaa aagcacgggg cgacgttcta cgatcagaat aatcagcgca aggatccgat
    11461 cgctattctg aaagagaacg gcgtcaacta tgtccgcctc cgtctgtggg tggatccgca
    11521 atcggcgagc ggcgaggact atggcggtgg gaacaatgat ctggccacca ccctggcgct
    11581 ggcgaagcgg gcgaaagtcc aggggatgaa actgctgctt gattttcact acagcgattt
    11641 ctggaccgat cccggcaagc agttcaagcc gaaagcttgg gaaaagatgg attacccgca
    11701 gctgaaaacg gcaattcatg actatacccg cgataccatc gcccgcttta agcaggcggg
    11761 cgtgctgccg gatatggtgc agattggcaa tgaaatcaac ggcggcatcc tgtggccgga
    11821 agggaaaagc tgggggcagg ggggcggcga gttcgaccga ctggccggcc tgctgaactc
    11881 ggccattgat ggcctgaagg agaatctacg taacggcgag catgtgaaaa tcatgctgca
    11941 tctggccgaa gggaccaaga acgatacctt ccgctggtgg tttgatgaga ttgacaagcg
    12001 ccatgtaccg tatgacgtga ttggtctgtc gatgtacacc tactggaatg ggccgatcag
    12061 cgccctgaag gccaatatgg atgacatcag caaacgctat aacaaagacg tcatcgtcgt
    12121 cgaggcggcc tatgcctata ccctagccaa ctgcgataac gcggaaaata gtttccaggc
    12181 gaaagaagaa aaggatgggg gatatcccgc caccgtacag gggcaatata actatattca
    12241 cgatttaatg caggcggtgg ttaatgtgcc tgaccagcgt ggtaaaggta ttttctattg
    12301 ggaaccaaca tggattgccg tgccgggaaa tacctgggcc acgccggcgg gaatgaaata
    12361 tatccatgac gagtggaaag agggtaatgc gcgtgaaaat caggcgctat ttgattgcca
    12421 gggaaaagta ttgccgtcgg taaaagtatt taattgattt ttttgtcgtt cttttcagga
    12481 agtttattat gaataaattt gcacctttac atccgaaggt taatacgctg ctgcatggcg
    12541 cggattataa tccggagcaa tgggagaatg accccgatat tattgataaa gacattgcca
    12601 tgatgcagca ggcaaaatgc aatgtgatgt cggtgggaat atttagctgg gcgaaactgg
    12661 agccacgcga aggggtgttt gatttcgcct ggctggacac tattctggat aaattgtatg
    12721 ccgccggtat acatgttttt ctggccacgc cgagcggcgc gcgtccggcg tggatgtcgc
    12781 aacgctatcc gcaggttctg cgggtggggc gcgatcgggt gccggccctg cacggcggcc
    12841 gtcacaatca ttgtatgtcg tcgccggtct atcgcgagaa gactctgaaa atcaatagcc
    12901 tgctggctga acgttattcc tcacacccgg cggtgctggg ctggcatatt tccaacgaat
    12961 atggcggcga atgccattgc gatctctgcc agaatcgttt tcgcgactgg ctgaaggcgc
    13021 gttaccagac cctggagaat ctcaaccagg cctggtggag caccttctgg agccatacct
    13081 atactgactg gtcgcagatt gaatcgcctg cgccgcaggg cgagatgtcg atccacggtc
    13141 tcaatcttga ctggcatcgc tttaacaccg ctcaggtgac cgatttttgc cgccatgaaa
    13201 ttgctccgct gaaagcggcg aattccgctc tgccagtgac caccaatttt atggaatatt
    13261 tctacgatta cgactactgg cagctggcgg aggtgctgga tttcatctcc tgggatagct
    13321 atccgatgtg gcaccgcgat aaagacgaaa ctgcgctggc ctgctacacc gcgatgtatc
    13381 acgacatgat gcgcagcctg aagggcggta agccgtttgt gctgatggag tccacgccgg
    13441 gcgccaccaa ctggcagccg accagcaaac tgaagaagcc gggaatgcat attctctcct
    13501 cgctgcaggc ggtggcgcat ggcgccgact cggtgcagta tttccagtgg cggaaaagcc
    13561 gcggttcggt tgagaaattt cacggcgcag tggtcgacca cgtcggacat attgataccc
    13621 gcattggccg cgaagtctgc cagctcggcg agatcctcag taagttgccg gaggtgaggg
    13681 gctgtcgcac cgaggcgaaa gtagcgatta tcttcgacca gcagaaccgc tgggcgctgg
    13741 atgacgccca ggggccgcgc aatcttggga tggaatatga gaagacggtc aacgagcact
    13801 accgcccgtt ctgggagcag ggcatcgccg tcgatgtgat tgacgccgat gtcgatttaa
    13861 cgccgtacca gttagtgatt gccccaatgt tatatatggt gcgcgacggc tttgccgggc
    13921 gggcggaggc gtttgtcgcc aacggcggcc acctggtgac cacctactgg accggcatcg
    13981 tcaatgagtc cgatctctgc tatctcggcg gcttcccggg cccgctgcgc aatctgctgg
    14041 ggatctgggc ggaagagatc gactgcctga atgacggcga gtttaatctg gtgcagggac
    14101 ttgccgggaa tcagtgcggt ctgcagggcc cttatcaggt gcgccatctc tgcgaactaa
    14161 tccatacaga gagcgcccag gcgctggcca cctaccggga tgatttttat gccggccggc
    14221 cggctgtgac ggtgaacgag ttcgggaaag gcaaagcctg gcatgtggcc tcccgcaacg
    14281 atttagcctt ccagcgcgat ttctttacca ccctgagcaa ggagctggcc ctgccgcggg
    14341 cgatagcgac ggagttacca cccggcgtgg tggctactgc gcgcaccgac ggtgacaact
    14401 cttttatctt cctgcagaac tacagcgccc aaaaccatac cctgaccctg ccgcaaggat
    14461 attgggattg cctgaccgac gcggcggtgt cggctccact gaccctgtcg gcatgggatt
    14521 gccgtattct ccgtcgtcac gcgtaatttc ttcttttcca cgcctgctct gacgagcagg
    14581 ctttttttat cttaaacctg gagtttatta tggtgagttt aaaatccttc ctgcactatt
    14641 tctccccagc gcgtccggca cagccattaa gcgaggcgga aaaacagcaa atcgaggcgc
    14701 ttattcaggc cttcggcggc gaagcgaata ttacgcaagt ggatgcctgt gttacgcgtc
    14761 tgcgcgtgac tgttcgtcat cttgcagacg tagatacgga cgcattacag cagcagggcg
    14821 cgctgggggt tattattctt tgtcaacagg tgcatgctat tttcggtaaa cagtccgatg
    14881 cgctgcgtca gcttctggat gaacattttg ctgcccggaa ataaataaaa cgggataacc
    14941 tgaaaacagg ttatcccgaa aataagacaa agacagcagt ggcaataaag tttaccggaa
    15001 gataattaac agtcttacag caggagataa aaataacgta tgagtcttaa ccgtgtgcga
    15061 ttaccaccag gcctcaacct gcacgccgaa attccaggta ttattcgccg tctggtcttc
    15121 aaacggttta ccatcttcgg agtcattcaa atacgaagca aaaatgcgta attccggacg
    15181 tgacataaag tctggcccgt ccgccaggcc caacgcaatg gtgtatttct cacccgcctg
    15241 tttgtagctg gtgccattgt tgtagctgtc tttctgatag aaaccgccaa cttcgccaat
    15301 caggcgcacg tagtcggtaa actggtactg tccgcgggcg accagcgaca gcatccgcgt
    15361 tttgtcggta tagtcggtaa tgtcatccgc cgagccccag gtcagaacgt ggttgataga
    15421 gaacttttca gtaatcggga tcaggccggt gttaatcaca cgataaccgg tggcgtcatt
    15481 cacgtagttc cacatatcgt accagccgcc gccctgggag accatgttct gcgccaggcc
    15541 tttgttggcg tactgcagca cggttttgtt atagccaccc agcatatcct gactgatttc
    15601 accggtcagc atgacgccgt tgtcagcatc gtacagtccg ccatagctat cctgtttttt
    15661 cgtggtgttt ggcatcgcgt agtcgatacc gaattcggtc cacgagcccg cccacggctt
    15721 ccagcccgcg tagcgtaaat cgatatagtt gatattgacg ttgctgtcgc catcgacgcg
    15781 atagtcaaca tcgttggcat cgccgcggat ccaggccagc gagacggcgc ccgggccgag
    15841 agtatagttt tccaccccgg ctccggagcc ggaaatgttc cagtatttgg tatcgatgat
    15901 atgcagatca tggcgctggt agtaacgttt accgccccag atcacagcat ttggatcgcc
    15961 agggatcagc ccctttatct gcaggttcaa ctgacgtaag ccgaactgtg catcatcgtt
    16021 cagcgtggtt tcattatcgt tagagccgtc ggagaccatg ctgaccatac tgtcgagata
    16081 gaagctgacg tcatctttct tatagacttc agagcccagc tccagctcgc cataggtatc
    16141 cgactcgttc cccaaacggc ccagcttatt tttttgccat tccacttcgc taccgtcgct
    16201 ggagactcca accccggcgc gcatatagcc atggaaatcg atcgttggga tcgcggcgga
    16261 agccgcaaac gcggaaggag caattagcgc agcggctaac gcgactgaaa gagcacgcag
    16321 tgtagtgttc atctctatcc tcttattgtt ttgtattacg ttcacataac taatgtaata
    16381 aaatgtctaa atgtaactac aggcaaatga gatatgattg ttttgtgact aactgcaaat
    16441 aatcacacta gtttttgttg cgataggtga ggttattcac aattcactgt attgtgaacg
    16501 taatactttt ttaggtgata ttttgcagcg tgaggtggcg gtgagcgttt ttttaaatgg
    16561 cccctgttac aatcatgaga taacaacata aggaaccgga gcatgaagtc taaaagcgct
    16621 actctggaag acgttgcccg tcatgcgggc gtctcttacc agaccgtgtc cagggtactc
    16681 aacaaatctg ccaatgtatc cgaagcgacg cgccgcaagg tggagaaatc gatagaggca
    16741 ttacgctatg tgccaaaccg gctggcgcag cagctggtgg gaaaggagag tcagacggtc
    16801 gggctggtga ctatttcgct ggcctggcat gcgccttccc aggtggcgac ggcggtaaaa
    16861 cgttatgcca atctggaagg ttatcaggtt ctgatttcga tgatcgatga gagcgtcaac
    16921 caaagtatcc aggattctat caatgagctg aaatctcagc gagtggacaa agtcattatc
    16981 aacgtgccac tggagaccga acaggcgcag aaaatcgccg ctgacaacga tgatatcgtc
    17041 tgcctgtttc tcgatgttga tccttacagc tccgtgttta acgtttcgtt taacccggcg
    17101 gacggcacgc gggccagcgt caaatatctc tatgagatgg ggcatcggga gatcgcgttg
    17161 ctggcaggac cggacaattc tgtctcggcc aaactccggt taaaaagctg gctggagact
    17221 ttgcagggct acggtctgaa gccggtgacc gtcctgcacg gcaactggga tgcgcaaagc
    17281 ggctatgccg gtgcgctgca gatgctgcgc gaaacgccca attttagcgc tgtgctggta
    17341 ggaaatgacc agatggcgtt gggggtgctc agcgccttcc atcagcatca ggtggcggtg
    17401 ccgggtgaga agtccgtcat tggctatgac gatacctacg aaagctcttt tttctatccg
    17461 tcgttgagta cggtgtcgct ggatctcgat ctgcagggga aggaggcggt gcggcggatc
    17521 ctcgccagca ccagcggtgc gccgcatacc tcatccattc tgcctgcgcg cttggtgata
    17581 cgccattcca gcggcgcccg aatcgagcag ggaaaagatc tgcaggccat tgcggaacag
    17641 ctccgcgcca tcgcccacac tctggcaccc tgacgccaaa ggcggggaaa ctcgccttat
    17701 taccttatcc ttaaattgag tagggtgtga tatttgtcac agcatggcgt aatattattg
    17761 aagtatccgg aattgagttt gggagaggcg ttttaatcca gagcagtcaa ttctagtgct
    17821 tatattgaaa gattaggaaa agtagccttt ctccagcaga tcgcaaagcg taggtatatg
    17881 ttgtcaacga cggatgaaaa atgcctcttc cgcaaccttg gtggaagaaa atggatgaaa
    17941 ctttctgatg aacaaggagc taagctgttt taagttatgg ataactgtag cagtctctta
    18001 agacactgct acagttatct tgcggatggc gctgcagtcg acaaattatt agctctgacg
    18061 gtataaccct ccatctccgc ggaaaacgca aagcagcaca atgtcctgaa tgcttttaag
    18121 cgtagcgact ttgttcatag ttgtcgtcgg cgccggatac agtatctacc ctgctcaggg
    18181 cagacgctat gacttgtatt ttccgtccgc cactggtact gccgtaaccc aacttgttca
    18241 cgaaaatttt ttgccgggtc gcttgccccc ttcgccggtt cacaccagca gtcttcacag
    18301 gcgttacaaa atttacaacg tcaacaggga ttaatagccg gaggtgaggc cgagaaactg
    18361 tcaggcacgc tccatatcgg tattgatgaa tgggcgtagc atcggggcca ccgttacggt
    18421 acgttaatcg tcaatcttga tactcacagt cccctcgtcc tgcttcccgg ctgcgatcag
    18481 cgtgcgctgg cgacctggtt cagaaaatat ccggagatag cccagcgtca ctttacgcag
    18541 cgtctcttcg gttgggcgct tcgccacgaa attaaactgc cctgctgggc tcgccgggcc
    18601 gtacagcgcg ccggtcaaac tgttaagcac atccatgcgc tcaagcattt ccaccggaaa
    18661 cgcggttgtt gaaacgatat tcagcccgtc cagacggctg ttggccacca cgctgccctg
    18721 catcccccga ctttgcgggc gcccgacatc cattccaccg cgcgcctgca tttgcgtact
    18781 gggagagtat ttcagcatct cgctgacgct ttgcgcctgc tgattttcaa tcatctcttt
    18841 agtaacagtg gtgctggaat atggtgtttc gatccaggat ttcgttccca gcgggccgac
    18901 atctatatcg ttcgttttga aaccggcacc ggcggcagaa tcggtggtgt cggcggcggt
    18961 gttgcccgtg acaatcattt catcttcaga agtagacgag gcatgagcat gaagcggtat
    19021 gaaagacgct aatgctggca aaagcgcagc gaaagttatt tgagtattca tgaatcactg
    19081 atcgttatgt aaatgtttat attacgatcg gtgcataggc aaaattatga tccagatcac
    19141 acgtgaatgt aagttattta caaattacgc taaccttctg tctccgctct ttgcagcata
    19201 aaagtatggc ggcgcgttgt tgaaagcagg agcggccggg ttgtcagaaa gctggtttgg
    19261 acgcaagtaa tatataacaa ctctggttac cgcatactgt caggacagta tctacttgag
    19321 cgtccgcctt tggcacaaag cggactgtca gattagattc agacctgtac tcattagctg
    19381 atacaaatca gctgcatata cctgcattcc aaataatttc agacagatat tatcgttgtg
    19441 aatcccccgc cacctttccg gtgcgcgggg ttttgtcttt tttcaccggg aatacatgta
    19501 tgaatccgtc tgatgccatt gaggcaattg aaaaaccact ttcttccctg ccttattcac
    19561 tttcccgtca catcctggaa catctgcgca aactcaccag tcacgaaccc gtgattggca
    19621 ttatgggtaa aagcggggcc ggtaaatcct cactctgtaa tgccctgttt cagggagagg
    19681 tcaccccggt cagtgatgtt cacgccggca cccgggaagt gcagcgcttc cgtctgagtg
    19741 gccatggtca cagcatggtt atcactgacc tgcccggggt gggcgagagc cgggacaggg
    19801 atgcagagta tgaggccctg taccgtgaca ttctgcctga acttgatctg gtactgtggc
    19861 tgattaaagc cgatgaccgt gccctgtctg tggatgagta tttctggcga cacattctgc
    19921 accggggaca tcagcaggtg ctgtttgtgg tgacgcaggc cgataaaacg gagccctgcc
    19981 acgaatggga tgtggccggc attcagcctt ctcccgcaca ggcacggaat attcgcgaga
    20041 aaacggaggc ggtattccgt ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc
    20101 gcaccggctg ggaactggat acgctggtca gtgcgctcat gacagcgctt cccgaccatg
    20161 ctgccagtcc cctgatgacc cgcctgcagg acgagctgtg cacggagtct gtccggtctc
    20221 aggcccgtga acagtttacc ggtgcggtgg accggatatt tgacacggcg gagagcgtct
    20281 gcattgcctc tgttgcacgc acggttctgc gtgccgtccg tgactcggtg gtctctgttg
    20341 cccgcgcggt atggaactgg attttcttct gaacccggct ggcaaaacac acggtttttg
    20401 cttcctgaat cttgtccgtc ctggcgttag gtaatagaat aaaacgatcg ataactattt
    20461 tatcgatcgt ttatatcgat cgataaagca ataatcaccc ctgtcagtaa ttctgaacag
    20521 acacatacag acatgagatt cagggaacag gaccacgctt cagaaacctc cgtctgccgg
    20581 acatccggca ctgtaaccct ttacctgccg gtatccacat ttgtgggtac cggctttttt
    20641 attcaccctc tgtaaggaaa aaaggatgaa acgacatctg aataccagct acagactggt
    20701 atggaatcac attacgggcg cttttgtggt ggcttccgaa ctggcccgct cacggggaaa
    20761 acgcgccggt gtggcggttg ccctgtccct tgccgctgcc acgtcactcc cggcaccggc
    20821 tgctgacacg gttgtacagg cgggagaaac cgtgagcggc ggaacactga taaatcatga
    20881 ccgccagact gtttcaggta cagcggatgg cgtgactgtc agtaccgggc tggagctggg
    20941 gccggattct gatgacaaca ccggcgggca gcagataact ccgagtggca ctgccagaaa
    21001 caccactgtc accgcagacg gtctgcagga tgtcatggcc ggtggcagtg ccacggatac
    21061 ggttatcagt gccggtggag gacagaatct gcgcgggcag gcttccggta cagtccttaa
    21121 cggcggtgag caatggatac atgcaggcgg cagtgcatcc ggaacggtga ttaaccggga
    21181 tggttaccag accattaaac atggcggact ggcaaccgga accatcgtca acaccggcgc
    21241 agaaggtgga ccggattctg aaaatgtctg taagcgtcaa cggagcaccg tattgacgct
    21301 tatttattgg tgagtactac gttccatggc aggagttcat caactcggtt ggaaggccat
    21361 tccggcagta cgctcaggat atggcgcaga tacgcttccg gatcgatacc gttcagacgg
    21421 caggtgccga tcagcccgta caacagtgct ccacgctcgc cgccgtgatc gctgccaaag
    21481 aacataaagt ttttctttcc gagacagact gcacgaagcg ctctttccgc agcattatta
    21541 tccgcctccg ccagaccgtc atcactgtaa taacagaggg catcccactg attcagtaca
    21601 tagctgaacg cttcgcccag tctggatttt ttcgacagcg taccattctt ctccacgatc
    21661 cattcatgca gcgacgtcag taacactttg cttcgctgct gcctgacggc aagacgctct
    21721 gactccggta atccccgtat ttcatcctcg atggcgtaca gttcactga
//
LOCUS       DBBGLQ010000072        21660 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.072, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000072 DBBGLQ010000000
VERSION     DBBGLQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.072"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            183..6289
                     /gene="irp2"
                     /locus_tag="ACIYSB_003630"
                     /pseudo
     CDS             183..6289
                     /gene="irp2"
                     /locus_tag="ACIYSB_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
     gene            6377..15868
                     /gene="irp1"
                     /locus_tag="ACIYSB_003631"
     CDS             6377..15868
                     /gene="irp1"
                     /locus_tag="ACIYSB_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HGC5430588.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWASEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGTRVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNKAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTSWETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESNGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLANIAHLLRLLTEHTPGESVPVPCLMVYAAGRPSRWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            15865..16965
                     /gene="ybtU"
                     /locus_tag="ACIYSB_003632"
     CDS             15865..16965
                     /gene="ybtU"
                     /locus_tag="ACIYSB_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HGC5430589.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            16962..17765
                     /gene="ybtT"
                     /locus_tag="ACIYSB_003633"
     CDS             16962..17765
                     /gene="ybtT"
                     /locus_tag="ACIYSB_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HGC5430590.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            17769..19346
                     /gene="ybtE"
                     /locus_tag="ACIYSB_003634"
     CDS             17769..19346
                     /gene="ybtE"
                     /locus_tag="ACIYSB_003634"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HGC5430591.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            19477..21498
                     /gene="fyuA"
                     /locus_tag="ACIYSB_003635"
     CDS             19477..21498
                     /gene="fyuA"
                     /locus_tag="ACIYSB_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HGC5430592.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         4359 a         6639 c         6237 g         4425 t
ORIGIN      
        1 gcgcccccct aaactcctcc ctgacagagg cccggatacc ccatccgggc ctctgttctt
       61 tccgcctgtt accaacccga aacgggcata aataaacccg tttcgggtag catccaattg
      121 ttaataatta ttattctcat atgagtaatg cttttcggta agacgtgcca tcaggaggaa
      181 gaatgatttc tggcgcacca tctcaggatt cgctgttacc ggacaaccgc cacgcggctg
      241 attaccaaca attacgcgag cggctcatac aggaactgaa tttaacgccg cagcagttac
      301 atgaagagag caacctgatc caggccggcc tggattccat aagattgatg agatggttac
      361 actggtttcg taaaaatggc taccgcctta cccttcgcga gctgtatgcc gcccccacgc
      421 tggcggcatg gaaccagtta atgctcagcc ggtcgccgga gaacgcggaa gaagaaacgc
      481 cgcccgacga atcatcctgg ccgaacatga ccgaaagtac ccccttccca ttgacgccag
      541 tacagcacgc ctacctgacg ggccgcatgc cggggcagac gcttggcggc gtgggttgcc
      601 acctgtatca ggagtttgaa ggccattgtc tgacggcgtc gcagctggag caggccatca
      661 cgaccttgct gcaacgccac ccaatgctgc atatcgcctt tcgccccgac gggcagcagg
      721 tctggctacc gcaaccttac tggaacggcg tcaccgttca tgatttacgc cataacgacg
      781 ctgaaagccg ccaggcctat ctggacgcac tgcgccagcg cctgagccac cgtcttttac
      841 gcgtggaaat cggcgaaacg tttgattttc agctgacgct cttgccggac aatcgccacc
      901 gcctccatgt caatattgac ctgctgatta tggatgcctc cagctttacg cttttcttcg
      961 atgagcttaa cgccctgctg gccggagaat cgctgccggc tatcgacacc cgctatgatt
     1021 tccgctcgta tttgctgcac cagcagaaga tcaatcaacc actgagagac gtcgcgcgcg
     1081 cttactggct ggcgaaagca tcgacgcttc cccccgcgcc cgtcttgccg ctggcctgcg
     1141 aacccgccac gctacgtgaa gtccgtaata cccgacgccg catgattgtc ccggcaacac
     1201 gctggcacgc ctttagcaac cgggccggcg agtatggcgt gacgccgaca atggcactgg
     1261 cgacctgttt ttctgccgtg ctggctcgct ggggcggcct gacgcgtctg ctgcttaaca
     1321 tcaccttatt cgaccgccag ccgctgcaac cggcggttgg cgcgatgctt gccgacttca
     1381 ccaatattct tctgctggat accgcctgcg atggcgatac cgtcagcaac ctggcgcgta
     1441 aaaaccagct cacgtttacg gaggactggg agcatcgcca ctggtccggc gtcgaattac
     1501 tccgtgaact caaacgccag cagcgctacc cccacggcgc cccggtggta tttaccagca
     1561 atctggggcg ttccctctac agcagccgcg cagaatcgcc gttgggcgag ccggaatggg
     1621 gcatctcgca aacgccgcag gtctggatag atcatctggc gttcgagcat cacggcgagg
     1681 tctggctaca atgggacagc aacgacgcgc tgttccctcc ggcgttagtc gaaacattgt
     1741 tcgacgccta ctgccagttg attaaccaac tctgcgatga cgaaagcgcc tggcaaaagc
     1801 cgttcgcaga tatgatgccc gccagccagc gcgcgatacg cgaacgggtc aacgccaccg
     1861 gcgcccccat tcccgaaggc ttgctgcatg aaggcatttt ccgtatcgct ctgcaacagc
     1921 cgcaggcgct ggcggtaacg gacatgcgtt atcagtggaa ttatcatgag ctgacagact
     1981 atgcccgccg ttgcgcgggc aggttaatcg agtgcggggt tcagcccggc gataatgtgg
     2041 ctatcacgat gtcgaaaggc gcaggacaac ttgttgcggt tctggccgtc ctgctggccg
     2101 gggcggttta cgttccggtt tcgctggatc agcctgccgc acggcgcgag aaaatctacg
     2161 ctgacgccag cgtccggctg gtgctcattt gtcagcacga cgccagcgcc gggtcagacg
     2221 atattcccgc ccttgcctgg cagcaggcca ttgaggcgga gccgatcgcc aacccggtag
     2281 tacgcgcccc cacgcaaccg gcctacatta tctacacctc cggctctacc ggtacgccga
     2341 aaggggtagt catttctcac cggggagcgc ttaacacctg ttgcgatatc aatacccgct
     2401 atcaggttgg cccgcatgac agggtgctgg ccctctccgc cctacatttt gatttatcgg
     2461 tttacgacat ttttggcgta ctgcgcgcgg gcggcgcgct ggtgatggtg atggaaaatc
     2521 aacggcgcga tcctcacgca tggtgtgagc tgatccagcg ccatcaggtc acgctctgga
     2581 acagcgtccc ggcgctgttc gatatgctgc tgacctggtg tgaaggtttc gccgacgcca
     2641 cgccggaaaa cctgcgcgca gtgatgcttt ccggcgactg gatcgggctt gacctccccg
     2701 cccgttatcg ggccttccgg ccacaaggac aatttatcgc gatgggcggc gccaccgagg
     2761 cgtctatctg gtctaacgcc tgcgaaattc acgacgtccc cgcccactgg cgctccatcc
     2821 cttacggttt tccgctaacc aaccaacgct accgggtggt ggatgaacag ggccgggact
     2881 gccctgactg ggtgccgggt gaattatgga ttggcggcat tggggtcgcg gaaggctatt
     2941 tcaacgatcc cctgcgtagc gagcagcaat ttttgacgct cccggacgag cgctggtatc
     3001 gcaccggcga tctcggctgc tactggccag atggcacaat cgagttcctc ggtcgtcgcg
     3061 acaagcaggt caaagtcgga ggatatcgca tcgagctggg cgaaatcgaa agcgcgctca
     3121 gccagctggt gggggtgaaa caagcaaccg ttctggcgat cggcgaaaaa gaaaaaacgc
     3181 tggcggcata cgttgttcct cagggcgagg cttttgcgtt accgatcatc ggaacccggc
     3241 actgccgcag gcgtggcaca cgcttgcggg aacgttgccc tgttgcgcca tctcgccaga
     3301 gatctccgca gaacaggtag ccgatttcct tcagcatcgc ctgctaaaac tgaagccggg
     3361 tcacaccgct ggcgccgatc ctctccccct gatgaactca ctcgctatcc agccgcgctg
     3421 gcaggccgtg gtggaacgct ggttagcatt tctggtgaca caacggcgac tgaagcccgc
     3481 tgctgaaggt tatcaggtct gcgctggtga agaacgcgag gatgagcatc cgcacttcag
     3541 cggacatgat ttaacgttat cgcaaattct tcgcggtgcc cgtaacgaac tgtcgttact
     3601 gaacgacgcg cagtggtcgc cggaaagcct ggcctttaac catccggcca gcgccccgta
     3661 tattcaggaa ctggcgacaa tttgccaaca gcttgcacag cgcttacagc gcccggtacg
     3721 cctgcttgag gtgggaaccc gcactggccg cgccgcagaa tcgctgttag cacagctcaa
     3781 cgccggacag attgagtatg tcgggcttga gcagagccag gagatgctgc tgagcgcccg
     3841 gcagaggctc gccccctggc ctggcgcccg tctgtccctc tggaatgcag acacgctggc
     3901 ggcgcacgct cactcggcgg acattatctg gcttaataac gccctgcatc gtctgctgcc
     3961 ggaagatccc gggctccttg cgacattaca acagcttgcc gttcccggcg cgctgctcta
     4021 cgtgatggag tttcgccagt taacgccgtc cgccctactc agcacgctcc tgttaaccaa
     4081 tgggcagccg gaggccttgc tgcataacag cgccgactgg gcggcattat ttagcgcggc
     4141 cggcttcaac tgtcagcatg gcgatgaggt cgcggggtta caacgcttcc tcgtacaatg
     4201 tcctgacagg caggtgcgcc gcgatccccg tcaacttcag gccgccctcg ccgggcgtct
     4261 gccggggtgg atggtgccgc aacggatcgt cttcctcgac gccttaccgc tgacagctaa
     4321 cgggaaaatt gactaccagg cgctgaagcg tcgtcatacc cctgaagcgg aaaacccggc
     4381 cgaagcggat ttaccccagg gcgacattga aaaacaggtt gccgccctct ggcagcaact
     4441 cttatcaact ggcaatgtca ccagagaaac cgacttcttc cagcaaggcg gcgatagcct
     4501 gctggcgacc cgtctgaccg ggcaacttca tcaggcaggt tatgaagcgc aattaagcga
     4561 cctgtttaat catccccggc tggcggattt tgccgccacg ctgcggaaaa ccgacgtccc
     4621 ggtcgaacaa ccattcgtcc actcccttga agatcgctac cagccctttg cgcttaccga
     4681 cgtgcagcag gcttacctgg tggggcgtca gccgggcttt gccctgggcg gcgtcggctc
     4741 acatttcttt gttgaatttg aaattgccga tctggacctc acccggctgg agacggtctg
     4801 gaaccgatta atcgcccgcc acgatatgct gcgcgccatc gtgcgtgatg gacagcaaca
     4861 ggtgctcgaa cagacgcccc cctgggtgat acccgcacac accctccata cgcctgaaga
     4921 ggcgttgcgg gtgcgcgaaa aactggcgca tcaggtactc aaccccgaag tgtggccggt
     4981 attcgatctc caggtcggat acgtggacgg gatgcctgcc cgcctgtggc tgtgtctgga
     5041 taacctgttg cttgacggtc tgagcatgca gatcctgctg gcggagctgg agcacggcta
     5101 ccgctacccg caacagctgc ttccgccgct gcccgtcacc ttcagggatt atctgcaaca
     5161 accctcgcta cagtcgccca atccagattc tctggcatgg tggcaggcgc agcttgatga
     5221 tattcctccg gcgcctgcgt tgccgctgcg ctgcttgcct caggaggttg aaacaccgcg
     5281 cttcgcccgc ctgaacggcg cactggacag cacgcgctgg catcggctga aaaaacgggc
     5341 ggctgacgcc catctcaccc cgtcggccgt actgttgtcg gtgtggtcaa cggttctctc
     5401 tgcatggagt gcacagcctg acttcacgct taaccttacg cttttcgaca ggcgaccgct
     5461 gcacccgcaa atcaaccaga ttctgggcga tttcacctcg ctgatgctgc tgagctggca
     5521 tcccggcgaa agctggctgc acagcgcgca gtcactacag cagcggctga gccagaacct
     5581 caaccaccgc gatgtgtcag ccatccgcgt gatgcgtcaa ctggcgcaac ggcaaaacgt
     5641 gccagccgtt ccgatgcccg tcgtctttac cagcgcgctg ggctttgagc aggataactt
     5701 cctcgcccgg cgtaatctgc tcaaaccggt ctggggcatc tcccagacgc cgcaggtctg
     5761 gctcgatcac cagatttatg aatccgaagg cgaactgcgc tttaactggg attttgtcgc
     5821 cgcgctgttt cctgccgggc aggtggagcg ccagtttgaa cagtattgcg cattgctaaa
     5881 ccgaatggcc gaggatgaaa gcggctggca actgccgctc gccgcgctgg tgcctcccgt
     5941 taaacacgca gggcaatgcg cagagcgctc accgcgcgta tgccctgagc actctcagcc
     6001 acacattgcg gcggacgaga gcaccgtcag cctgatttgc gacgccttcc gcgaggtggt
     6061 tggcgagtct gtcacgcccg cagaaaactt ctttgaggcg ggcgcaacgt cgctgaatct
     6121 ggtgcaactg cacgttttgt tacaacgtca cgaattttcc accctgacgt tgcttgacct
     6181 cttcacccac ccttctcctg ctgccctggc cgattatctg gccggcgtcg ccacggtgga
     6241 gaaaacacaa cgacctcgcc ctgttcgccg tcgtcagcgg cggatatagc gcgaagcaaa
     6301 ctgattttcc ccggaacgcc atcgcgaacg catggcgttc cattgacttt gtgaatctta
     6361 ggaaacgggg ccgattatgg ataacttgcg cttctcttct gcgccgacag cagattccat
     6421 tgatgcatcg atcgctcaac actacccgga ctgcgaacct gtcgcggtta tcggctacgc
     6481 ctgccatttt cctgaatcgc cggatggcga aacgttctgg caaaatctgc tggaaggtcg
     6541 tgaatgcagc cgacgcttta cgcgcgaaga gcttctggcc gtcggtctgg atgccgccat
     6601 cattgacgat cctcattatg tcaatatcgg tacggtgtta gacaacgccg actgcttcga
     6661 cgccaccctg tttggctatt cgcgacagga agcggagtcg atggacccgc agcagcgcct
     6721 gtttttgcag gcggtctggc atgcgctgga acatgccggt tatgcccccg gcgccgtccc
     6781 ccataagacc ggcgttttcg cctcttcccg gatgagtacc taccccggtc gcgaagcatt
     6841 gaacgtgaca gaagtcgcgc aggtaaaagg tctgcaatct ctgatgggca atgataaaga
     6901 ctatattgcc acccgcgccg cgtacaaact caacctgcac ggcccggcgt tatcggtaca
     6961 gaccgcctgc tccagctcgc tggttgccgt gcatctggcc tgtgaaagcc tgcgcgctgg
     7021 cgaatccgat atggcggttg ccggcggcgt ggcgctctct ttcccccagc aggcaggcta
     7081 ccgctaccag cccggaatga ttttctctcc tgatggtcac tgtcgtccct ttgacgcctc
     7141 ggctgagggc acctgggccg gtaacggtct cggctgcgtg gtgctgcgtc gcctgagaga
     7201 cgcgctgctg tcaggcgatc cgattatctc ggtgatcctc tccagcgcgg tcaacaacga
     7261 cggcaacaga aaggtcggct ataccgcccc ttccgtcgca gggcaacagg cagtcatcga
     7321 agaggcgtta atgctggcgg ccatcgacga caggcaggta ggttacattg aaacccacgg
     7381 caccggcaca ccgctgggcg acgcgattga aattgaagcg ttacgcaacg tctatgcgcc
     7441 tcgcccgcag gatcagcgct gtgcgctcgg ttccgtgaaa agtaacatgg gccatctgga
     7501 taccgcggcg ggcattgccg gactgctgaa aaccgttctg gcagtcagtc gcgggcaaat
     7561 tcctccctta ctgaattttc acacccccaa cccggcgctg aaacttgaag agagcccctt
     7621 taccataccg gtgtcggcac aggcatggca ggacgaaatg cgctatgcgg gcgtctcctc
     7681 ctttggtatt ggcggcacca actgccatat gatcgtcgcc tcgctgcccg acgcgctcaa
     7741 cgcgcgcctc cccaatacgg atagcggcag aaaaagtacc gcgctgctgc tcagcgccgc
     7801 cagcgacagc gcgttgcggc ggctggcgac ggattatgcc ggggcgctga gagagaatgc
     7861 ggatgccagc tctctggcct tcacagccct gcacgcgcgc cgtctcgatc tccccttccg
     7921 cctggcggcg ccattaaacc gtgaaaccgc cgaggcgctc agcgcctggg ctagtgagaa
     7981 atcgggggcg ctggtttaca gcggccacgg cgccagcggc aagcaggtgt ggctgtttac
     8041 cggccagggc tcgcactggc gcactatggg tcaaacgatg taccagcact caacggcgtt
     8101 tgccgacacg ctggatcgct gtttttccgc ctgtagcgaa atgctcacgc cgtcactgcg
     8161 cgaagcgatg tttaaccccg attcggcgca gctggacaat atggcctggg cgcagccggc
     8221 gattgtcgcg tttgaaatcg cgatggcggc gcactggcgt gctgaaggac tgaagccaga
     8281 cttcgccatt gggcattccg tcggtgaatt tgccgctgcc gttgtctgcg gacactatac
     8341 gattgaacag gtcatgccac tggtttgtcg gcgcggcgcg ctaatgcagc agtgcgcaag
     8401 cggcgcgatg gtggcggtat ttgcagacga agacacgctg atgccgctgg ctcgccagtt
     8461 tgagctggat ctcgccgcca acaacggtac gcaacatacg gtattttccg ggccggaagc
     8521 ccgtctcgcg gtattttgcg ccacgctctc gcagcatgac attaactatc gtcgcctgag
     8581 cgtaaccggt gcggcgcact ccgctttact ggagccgata ctcgatcggt tccaggacgc
     8641 ctgcgcggga ctgcacgcgg agccggggca aataccgatt atttccacgc tcaccgccga
     8701 cgtcattgat gagtcaacgc tcaaccaggc ggattactgg cgccgacaca tgcgccagcc
     8761 ggtgcgtttt atccagagta ttcaggtggc gcatcagctc ggcacccgcg tttttctgga
     8821 gatggggccc gatgcccagt tggttgcttg cgggcagcgc gaataccgcg ataacgcata
     8881 ctggatagcc agcgcccggc gtaacaaaga ggcgagcgat gtcctcaatc aggccctgct
     8941 ccagctttac gctgccggcg tcgccctacc gtgggccgac ctgctggcgg gcgatggaca
     9001 acgtatcgct gcgccatgtt atccgtttga tactgaacgt tactggaaag agcgcgtctc
     9061 cccggcctgc gagcctgccg acgcagcgct gtctgccggg ctggaggtgg cgagtcgcgc
     9121 cgcgacagcg ctcgatctcc ctcgcctgga agcgcttaaa cagtgcgcca cgcgactgca
     9181 cgccatctac gtcgatcaac tggtacaacg ctgtaccggc gatgccatag agaacggcgt
     9241 ggacgccatg accatcatgc gccgtggacg tctgctgccc cgctaccagc agctactcca
     9301 gcgcctgctg aataactgcg tggtcgacgg cgattaccgc tgcaccgacg ggcgatacgt
     9361 ccgcgcccgc cccattgaac atcaacagcg ggaatcactg ctgacggaac ttgccggtta
     9421 ttgtgaaggt tttcaggcta ttcccgacac catcgcccgt gccggcgatc ggttatatga
     9481 aatgatgagc ggcgcggaag aaccggtggc gattatcttc ccgcaaagcg cctccgacgg
     9541 cgtggaagtg ctgtatcagg aattcagctt tggccgctat ttcaaccaaa tcgccgccgg
     9601 ggtattacgc ggcattgtcc agacgcgtca gccccgccag ccgttgcgta ttcttgaagt
     9661 tggcggcgga accggcggca ccaccgcgtg gctgctgccg gaactcaacg gcgttccggc
     9721 actggagtac catttcaccg atatctcggc gctgttcacc cgtcgcgccc agcagaaatt
     9781 cgccgactat gattttgtga agtatagcga gctggatctc gaaaaagagg cgcagtctca
     9841 gggtttccag gcacagtctt acgatcttat cgtggcagcg aacgtgattc acgccacccg
     9901 ccatattggc cgcacgctcg ataatctgcg ccccctgctc aagccgggcg ggcgcctgct
     9961 gatgcgcgaa atcacccagc caatgcgtct gtttgacttc gttttcggcc cgctggttct
    10021 tccgctacag gatctcgacg cccgcgaagg tgagttattc ctcaccaccg ctcagtggca
    10081 acaacagtgc cgccacgccg gattcagcaa agtggcgtgg ctaccgcagg atggcagccc
    10141 gaccgccggg atgagcgaac atatcattct cgccacgctg cccggtcagg cggttagcgc
    10201 cgtaacattc accgcgccat cagaacccgt gttggggcag gcgctgacgg ataacggtga
    10261 ttatctcgcc gactggtctg attgcgcagg tcagcccgaa cagtttaacg cccgctggca
    10321 ggaggcctgg cgtctgcttt cacagcgtca tggcgacgct ctgcctgtgg aaccgccccc
    10381 cgtcgccgcc ccggagtggc tggggaaggt tcgcttaagc tggcaaaaca aagccttttc
    10441 ccgcggtcag atgcgcgttg aagcccgtca tcctgctggc gagtggctgc cgctatcgcc
    10501 cgccgcgcct cttcctgcgc cgcaaacgca ttatcaatgg cgctggacgc ccctcaacgt
    10561 cgccagcatt gaccatccgc ttacctttag cttcagcgcc ggtacgcttg cgcgcagcga
    10621 cgagctggcg caatacggca tcattcacga tccgcacgcc tcttcacgac tgatgattgt
    10681 tgaggagagc gaggatacgc tggccttagc ggagaaagtg atagcagcgc tcaccgccag
    10741 cgcagccgga ttgattgtgg ttactcgccg cgcgtggcga gtcgaggaaa atgaagcact
    10801 ctctgcatcc catcacgcgc tatgggcctt gcttcgcgtc gcggccaacg aacagccgga
    10861 acggttgctt gccgccatcg atctcgccga aaacacctcg tgggaaacgc tgcatcaagg
    10921 gttgagcgca gtctcactat cacagcgctg gctcgccgca cggggtgaca ccctttggct
    10981 cccttcactg tcgcccaata cgggatgcgc cgctgaatta ccggcaaacg tgtttaccgg
    11041 cgatagccgc tggcatctgg tgaccggagc gtttggcgga ttaggccgcc ttgccgtgaa
    11101 ctggctcaga gaaaaagggg cgcgacgcat cgccctgctg gcgccgcgcg tggatgagtc
    11161 atggctacgc gacgtggagg gcgggcagac gcgcgtctgc cgttgtgatg tgggcgatgc
    11221 cgggcaactg gccacggttc ttgacgatct ggcggccaac ggcggcattg ccggagcgat
    11281 tcatgccgct ggcgtattgg ctgacgcgcc cttgcaggag cttgatgacc accagctggc
    11341 tgccgttttc gcggtaaaag cgcaggcggc aagccagctg ttgcaaaccc tgcgcaacca
    11401 cgacggacgc tatcttattc tctactcttc cgctgccgcc accctcggcg cgccgggtca
    11461 gagcgcccat gcgctggcct gcggctacct ggacgggctg gcccagcagt tttccaccct
    11521 tgatgcgccg aaaacgctct ctgtcgcctg gggcgcatgg ggagaaagcg gtcgggcggc
    11581 cacgccggaa atgctggcga cgctcgccag ccgaggtatg ggcgcgttaa gcgatgccga
    11641 aggctgctgg cacctggaac aggcggtgat gcgcggcgcc ccgtggcgac tggcgatgcg
    11701 cgtttttacc gacaaaatgc ccccgttaca acaggctctg tttaacatca gcgccacaga
    11761 aaaagccgca acgccggtca ttcctcctgc tgatgacaac gcctttaacg gcagcctgag
    11821 cgatgaaaca gcggtgatgg catggctgaa aaagcggatt gcggttcagc taaggctgag
    11881 cgatccggcg tcactgcatc caaaccagga tctgttgcaa ctcggcatgg actcgctgct
    11941 cttccttgaa ctcagtagcg atattcagca ctacctgggc gtacgcatca atgcggaacg
    12001 ggcgtggcag gatctgtctc ctcatggact cacgcagctt atctgttcta agccagaggc
    12061 gacgcctgcc gcttcgcagc cggaagtgtt gcggcacgac gccgacgagc gttatgcgcc
    12121 cttccctttg acgcccattc agcacgccta ctggctgggg cgaacccacc tcattggcta
    12181 tggcggcgtc gcctgtcacg tcctgtttga gtgggataaa cgccacgatg agttcgatct
    12241 cgccatactg gagaaagcat ggaaccagct catcgcacgc cacgatatgt tgcgtatggt
    12301 ggttgatgcc gacgggcagc agcgaatcct ggcgacaacg ccggagtatc acatcccgcg
    12361 tgacgatctg cgcgcgcttt ccccggaaga acagcgcatc gcgctggaaa aacggcggca
    12421 tgaactgagc tatcgcgttt tgcctgccga ccagtggcct ctttttgagc tggtggtcag
    12481 cgaaatcgac gattgccatt accgtctgca tatgaacctc gaccttttgc agtttgatgt
    12541 gcagagtttt aaagtcatga tggacgacct ggcgcaggtc tggcgcggtg aaacgctggc
    12601 gccgctcgct attaccttcc gtgattatgt gatggctgaa caggcgcgcc gacagacatc
    12661 ggcatggcac gatgcctggg attactggca ggaaaaactg ccgcaactgc ccttagcgcc
    12721 agagctgccg gtggttgaga cgcccccgga aacgccacac ttcaccacct tcaaatcgac
    12781 gatcggcaag acagaatggc aggccgtgaa acagcgctgg cagcagcaag gcgtcacacc
    12841 gtctgccgcg ctgctcacgc tgtttgccgc tacccttgag cgctggagcc gtaccacaac
    12901 atttacgctg aacctgacgt tcttcaatcg ccagccgatc catccgcaaa tcaaccagtt
    12961 gattggtgat tttacctccg tcacgctggt tgattttaac ttctcagcgc cggtgacgtt
    13021 gcaagagcag atgcaacaga cccaacagcg cctctggcaa aacatggcgc acagtgaaat
    13081 gaacggtgtt gaggtgatcc gtgagctggg ccgcctgcgc ggatcacaac gtcaaccgct
    13141 gatgccggta gtgtttacca gtatgctggg gatgacgctg gaaggcatga ctatcgatca
    13201 ggcgatgagc catctgttcg gcgaaccctg ctatgtattc acgcaaacgc cgcaggtctg
    13261 gctggatcat caggtcatgg agagcaacgg cgagttgatg tttagctggt actgcatgga
    13321 caacgtgctg gaacccggcg ctgccgaggc gatgtttaat gactattgcg ccatcctgca
    13381 agccgtcatc gccgcccctg aaagcctgaa gactctcgcc agcggcatcg ccaggcacat
    13441 tccccgccga cgctggccgc tgaacgcgca ggcggactac gacctgcggg atattgagca
    13501 ggcgacgctc gaataccccg gcatccggca ggccagagcg gaaataaccg aacagggcgc
    13561 gttgacgctg gatatcgtga tggccgacga tccttcgcca tcagcggcga tgcctgatga
    13621 gcacgaactt acccaactgg cgctgccgtt gcctgagcag gcgcagcttg atgagctgga
    13681 ggcgacctgg cgctggctgg aggcgcgtgc gctacagggg atcgcggcta cgctaaatcg
    13741 tcacggcctg tttaccacgc cggagatcgc ccatcgcttt agcgcaatag tacaggcgct
    13801 gtccgcgcaa gcgtctcacc agcgtctgct gcgccagtgg ctacagtgtc tgacggaaag
    13861 agagtggtta atccgcgaag gtgaaagctg gcgctgccgc attccgctca gcgagattcc
    13921 tgagcctcag gaagcgtgcc cgcaaagcca atggagccag gcgctggcgc agtatctgga
    13981 aacctgcatc gcccggcacg acgccctctt ctccgggcag tgttctccgc tggaattgct
    14041 gttcaacgag cagcatcgcg ttaccgacgc gctgtatcgc gacaaccccg ccagcgcctg
    14101 tctgaatcgc tataccgcgc agattgccgc cttgtgcagc gcagaacgga ttctggaggt
    14161 tggcgccgga accgcagcca ctaccgcgcc ggtgctgaag gccacgcgga acacgcgaca
    14221 gtcgtaccac ttcacggacg tctccgcgca gttcctcaat gacgccagag cccgtttcca
    14281 tgatgaatcg caggtgtctt atgccttgtt cgacatcaac cagccgctgg atttcaccgc
    14341 ccacccggag gcgggttacg acctgatcgt tgccgtcaat gtgctccacg acgccagcca
    14401 tgtcgtccag acgttgcgca gattaaaact gttgctgaaa gccggcggac gtttgctgat
    14461 cgttgaagcg acggagcgaa acagcgtatt ccagctggcg agcgtgggct ttattgaggg
    14521 attaagcgga taccgcgatt tccgccgccg ggatgagaaa ccgatgctca cccgctccgc
    14581 atggcaggag gttctcgttc aggccgggtt tgcaaacgag ctggcgtggc ccgcgcagga
    14641 atcgtcgccg ctgcgccagc atctgctggt agcgcgttcg cctggcgtaa atcgcccgga
    14701 taaaaaagcc gtgagccgct atttacagca gcgctttggc accggtctgc ccattttaca
    14761 gatccggcaa agagaagcgt tatttacgcc gctgcatgcc ccgtctgatg cgccgactga
    14821 gccagccaaa cccacgccag ttgccggggg gaatccggcg ctggaaaaac aggtggctga
    14881 actctggcaa tcgctgctgt ctcgccccgt ggcaaggcat cacgactttt tcgaactggg
    14941 cggcgacagc ctgatggcga caaggatggt cgcgcagcta aaccggagag ggattgccag
    15001 ggctaacctt caggatctgt tcagccattc gacgctgagc gacttctgcg cccatctaca
    15061 ggcggctacg tcaggagagg acaacccgat ccccctttgc cagggcgacg gtgaggaaac
    15121 cctgtttgtc ttccacgctt cagacggcga tatcagcgcc tggctgccgc tcgctagcgc
    15181 gttgaacagg cgcgttttcg gcctgcaagc aaaatcgccg cagcgctttg ccacgctcga
    15241 ccagatgatc gatgagtatg tcgggtgcat ccgtcgtcag cagcctcacg gcccttatgt
    15301 gctggcgggt tggtcgtatg gcgcgtttct tgcggcgggc gccgcacaac gcctgtacgc
    15361 caaaggcgag caggttcgga tggtgttaat cgatcccgtg tgccgacagg atttctgttg
    15421 cgaaaaccgg gcggccctgc tgcgcctgtt agccgaagga caaacgcctc tggcactgcc
    15481 cgaacatttc gaccagcaga cgcccgacag ccagcttgcc gactttatca gcctcgctaa
    15541 aacggccggt atggtgtcgc aaaacctgac gctgcaagcg gcagaaacgt ggctcgccaa
    15601 catcgcgcat ctgctgcgtt tactgactga gcatacgccg ggcgaaagcg ttccggtccc
    15661 ctgtctcatg gtgtatgccg ccgggagacc ctcgcgctgg acgccagcag aaaccgagtg
    15721 gcagggctgg ataaacaacg ccgacgacgc tgtgattgaa gccagccact ggcaaatcat
    15781 gatggaagcc ccccacgttc aggcttgtgc gcaacacatt acgcgctggc tttgcgcaac
    15841 ctcaacgcaa ccggagaaca cgttatgatg ccgtccgcct ccccaaaaca acgcgtactg
    15901 attgtgggcg ccaaatttgg cgaaatgtac ctgaatgcct ttatgcagcc cccggagggg
    15961 ctggaactgg tcggcctgct ggcgcaggga agcgcccgtt caagagagct ggctcatgcg
    16021 tttggcattc cgctgtatac ctcgccggaa cagataacca ggatgccgga tatcgcctgt
    16081 attgtcgtgc gttcgaccgt cgccggagga accggtacgc agcttgccag acactttctg
    16141 acgcgcggcg tacacgtcat tcaggagcat cccctccacc cggatgacat cagttcctta
    16201 caaacgctgg cgcaggaaca gggctgctgc tattgggtaa acacgtttta tccgcatacg
    16261 cgcgcgggac gcacatggct tcgcgatgcg cagcagctgc gtcgctgcct ggctaagacg
    16321 ccgccggtag tccacgccac caccagccga cagttgctct actccactct ggatttgttg
    16381 ttgctggctc tgggcgttga tgccgccgcc gtggagtgcg acgtggttgg cagctttagc
    16441 gattttcact gtctgagact cttctggccg gagggagaag cctgcctgct gcttcagcgc
    16501 tatctcgatc ctgacgatcc agatatgcat agccttatca tgcaccgcct gctgctgggc
    16561 tggccggaag gtcatctttc gctggaggcg agctacggcc ccgtcatctg gtcatccagc
    16621 ctgtttgtcg cagaccatca ggagaacgcc cacagcctct accgcagacc ggagatcctg
    16681 cgcgatctgc cgggtctgac gcgcagcgcg gcgcccctga gctggcgcga ctgctgcgaa
    16741 accgtcgggc cggagggcgt cagttggttg ctacaccagt tgcgcagcca tctggcgggc
    16801 gaacatccac cagcggcctg ccaaagcgta catcagatcg cgttatcacg tttatggcag
    16861 cagattttac gtaagaccgg caatgcggag atcagacgcc tgacgccgcc gcaccacgac
    16921 cggttagccg gtttctacaa cgatgatgat aaggaggcgc tgtgacgcaa tctgcaatgt
    16981 gcatcccgct gtggcccgcc cggaacggca atactgcgca tctggtcatg tgccctttcg
    17041 ctggcggcag cagtagcgcg tttcgccact ggcaagctga gcaactggct gattgcgcgc
    17101 tttctctggt gacctggccg gggcgcgatc gccttcgcca tctggaaccg ctcagaagca
    17161 ttacacaact ggcggcactg ctggcgaacg agctggaagc atccgtatcg cctgacacgc
    17221 cgcttttact cgccgggcac agcatggggg cgcaggtggc gtttgaaacc tgccgacttc
    17281 tggagcaacg ggggcttgcg ccacaaggac tgattatttc cgggtgccat gccccgcatc
    17341 tgcattctga acgccagctc agccatcgcg atgatgccga ctttatcgct gagctgatag
    17401 acattggcgg atgttctcct gaactgcggg aaaaccagga attaatgtcg ctgtttcttc
    17461 ctcttctgcg cgctgatttt tacgccaccg agagctatca ctacgactcg cccgacgtct
    17521 gtccgccgct gcgcacgcct gcgctgttat tgtgcggcag ccacgatcgc gaagcctcct
    17581 ggcagcaggt cgatgcctgg cgtcagtggc tgagccacgt tacaggcccg gtggtgattg
    17641 acggcgatca tttctatccc attcaacaag cccggtcctt ttttacgcag attgtccgcc
    17701 attttcccca cgcattttct gcaatgaccg cgttgcaaaa acagcccagt acttcagaaa
    17761 ggtgacgcat gaattcttcc tttgaatctc tgattgaaca gtatccctta cccattgccg
    17821 aacagttgcg ccactgggcg gcccgttatg cctcgcgaat tgccgtcgtt gatgcaaagg
    17881 ggtcgttaac ctacagcgcg cttgatgcac aagttgacga acttgccgca ggtctgtcat
    17941 cactgggttt gcgttcgggg gagcatgtaa ttgtgcagct tcccaacgac aacgcgtttg
    18001 ttaccctgct gttcgccttg ttaagactgg gcgttatccc cgtgctggcg atgccctcgc
    18061 aacgggctct ggatatcgac gcgctgattg agctggcgca acccgtcgct tacgttattc
    18121 acggggaaaa ccacgcagag ctggcccgac agatggcgca caaacacgcc tgcttgcgtc
    18181 atgttctggt cgctggagag accgtgagcg acgattttac gccgctcttc tcccttcacg
    18241 gtgagcgaca ggcatggccg cagcctgatg tttccgccac cgcgttgttg ttgctctcag
    18301 gcggcacaac cggcacgccc aaactcatcc cgcgccgaca tgccgactat agctataact
    18361 tcagcgcttc tgctgaactg tgcggcatca gccaacagag cgtgtatctc gccgtcctcc
    18421 cggtggcgca taactttccg ctggcctgcc ccggtattct gggaacgctt gcctgcggcg
    18481 gaaaagtggt gctgaccgac agcgccagct gtgatgaggt gatgccttta atcgcgcagg
    18541 aaagagtgac tcacgtcgcc ctggttccgg cgctggcgca attatgggtg caggccaggg
    18601 agtgggaaga cagcgacctt tcgtcgctgc gcgtcattca ggcaggcggc gcccggctcg
    18661 acccgacgct tgctgagcag gttatcgcca cctttgactg taccctgcaa caggttttcg
    18721 gtatggcgga aggcctgctc tgttttaccc ggctggacga tccgcatgcc accattctcc
    18781 acagccaggg gcgcccgttg tcccctctgg atgaaatccg catcgttgat caagacgaga
    18841 acgacgtcgc gccgggcgaa accgggcaat tgttaacgcg cggcccttat accatttcgg
    18901 gctattaccg cgcccctgcc cacaacgcgc aggcctttac cgcgcaaggg ttttaccgca
    18961 caggcgacaa tgtcaggctg gatgaggtgg ggaacctgca cgttgaggga cgcataaaag
    19021 agcagatcaa ccgcgccgga gaaaaaatag ccgcggctga agtggaatcg gcactgctgc
    19081 gtttagcgga agtgcaagat tgcgcggtgg tcgccgcgcc ggacacgctg cttggcgagc
    19141 ggatttgcgc gtttatcatc gcgcagcagg tgccaactga ctatcagcag ttgcgtcaac
    19201 aactgacccg tatggggctc agcgcgtgga aaattcctga ccaaatcgag tttctggacc
    19261 actggccgct caccgccgtc ggcaagatag acaaaaaacg cctgacggct ctcgccgtcg
    19321 accgttatcg ccattctgcc caataagcgc aaaccgaccc gaaacaggtt gaaataaacc
    19381 cgtttcgggt agcactacta ttagaaatag ttatcatttt caattcacca ttgtcggtat
    19441 ttttggcgtt tcgccgtctt acagggactc acaacaatga aaatgacacg gctttatcct
    19501 ctggccttgg ggggattatt gctccccgcc attgctaatg cccagacttc acagcaagac
    19561 gaaagcacgc tggtggttac cgccagtaaa caatcttccc gctcggcatc agccaacaac
    19621 gtctcgtcta ctgttgtcag cgcgccggaa ttaagcgacg ccggcgtcac cgccagcgac
    19681 aaactcccca gagtcttgcc cgggctcaat attgaaaata gcggcaacat gcttttttcg
    19741 acgatctcgc tacgcggcgt ctcttcagcg caggacttct ataaccccgc cgtcaccctg
    19801 tatgtcgatg gcgtccctca gctttccacc aacaccatcc aggcgcttac cgatgtgcaa
    19861 agcgtggagt tgctgcgagg cccacaggga acgttatatg gcaaaagcgc tcagggcggg
    19921 atcatcaaca tcgtcaccca gcagccggac agcacgccgc gcggctatat tgaaggcggc
    19981 gtcagtagcc gcgacagtta tcgaagtaag ttcaacctga gcggccccat tcaggatggc
    20041 ctgctgtacg gcagcgtcac cctgttacgc caggttgatg acggcgacat gattaacccc
    20101 gcgacgggaa gcgatgactt aggcggcacc cgcgccagca tagggaatgt gaaactgcgt
    20161 ctggcgccgg acgatcagcc ctgggaaatg ggctttgccg cctcacgcga atgtacccgc
    20221 gccacccagg acgcctatgt gggatggaat gatattaagg gccgtaagct gtcgatcagc
    20281 gatggttcac cagacccgta catgcggcgc tgcactgaca gccagaccct gagtgggaaa
    20341 tacaccaccg atgactgggt tttcaacctg atcagcgcct ggcagcagca gcattattcg
    20401 cgcaccttcc cttccggttc gttaatcgtc aatatgcctc agcgctggaa tcaggatgtg
    20461 caggagctgc gcgccgcaac cctgggcgat gcgcgtaccg ttgatatggt gtttgggctg
    20521 taccggcaga acacccgcga gaagttaaat tcagcctacg acatgccgac aatgccttat
    20581 ttaagcagta ccggctatac caccgctgaa acgctggccg catacagtga cctgacctgg
    20641 catttaaccg atcgttttga tatcggcggc ggcgtgcgct tctcgcatga taaatccagt
    20701 acacaatatc acggcagcat gctcggcaac ccgtttggcg accagggtaa gagcaatgac
    20761 gatcaggtgc tcgggcagct atccgcaggc tatatgctga ccgatgactg gagagtgtat
    20821 acccgtgtag cccagggata taaaccttcc gggtacaaca tcgtgcctac tgcgggtctt
    20881 gatgccaaac cgttcgtcgc cgagaaatcc atcaactatg aacttggcac ccgctacgaa
    20941 accgctgacg tcacgctgca agctgcgacg ttttataccc acaccaaaga catgcagctt
    21001 tactctggcc cggtcgggat gcagacatta agcaatgcgg gtaaagccga cgccaccggc
    21061 gttgagcttg aagcgaagtg gcggtttgcg ccaggctggt catgggatat caatggcaac
    21121 gtgatccgtt ccgaattcac caatgacagt gagttgtatc acggtaaccg ggtgccgttc
    21181 gtaccacgtt atggcgcggg aagcagcgtg aacggcgtga ttgatacgcg ctatggcgca
    21241 ctgatgcccc gactggcggt taatctggtc gggccgcatt atttcgatgg cgacaaccag
    21301 ttgcggcaag gcacctatgc caccctggac agcagcctgg gctggcaggc gactgaacgg
    21361 atgaacattt ccgtctatgt cgataacctg ttcgaccgtc gttaccgtac ctatggctac
    21421 atgaacggca gcagcgccgt cgcgcaggtc aatatgggtc gcaccgtcgg tatcaatacg
    21481 cgaattgatt tcttctgatt attgtaaaag ggataccgaa aaggtatccc ttttacacca
    21541 ctagttaaaa ccagtaactc agcagagtcg caaaaaatat taatccatag tgattattta
    21601 aacaatgaaa ttgcgattag ggtaaataga tttaactttc tcgttccttt ctctccttat
//
LOCUS       DBBGLQ010000073        21536 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.073, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000073 DBBGLQ010000000
VERSION     DBBGLQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.073"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..218)
                     /locus_tag="ACIYSB_003636"
     CDS             complement(<1..218)
                     /locus_tag="ACIYSB_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430593.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACIYSB_003637"
     CDS             complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACIYSB_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGC5430594.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(828..1634)
                     /gene="nikE"
                     /locus_tag="ACIYSB_003638"
     CDS             complement(828..1634)
                     /gene="nikE"
                     /locus_tag="ACIYSB_003638"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGC5430595.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGEKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1631..2395)
                     /gene="nikD"
                     /locus_tag="ACIYSB_003639"
     CDS             complement(1631..2395)
                     /gene="nikD"
                     /locus_tag="ACIYSB_003639"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGC5430596.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2395..3228)
                     /gene="nikC"
                     /locus_tag="ACIYSB_003640"
     CDS             complement(2395..3228)
                     /gene="nikC"
                     /locus_tag="ACIYSB_003640"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGC5430597.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3225..4169)
                     /gene="nikB"
                     /locus_tag="ACIYSB_003641"
     CDS             complement(3225..4169)
                     /gene="nikB"
                     /locus_tag="ACIYSB_003641"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGC5430598.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4169..5743)
                     /gene="nikA"
                     /locus_tag="ACIYSB_003642"
     CDS             complement(4169..5743)
                     /gene="nikA"
                     /locus_tag="ACIYSB_003642"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGC5430599.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPSQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5854..6441)
                     /gene="acpT"
                     /locus_tag="ACIYSB_003643"
     CDS             complement(5854..6441)
                     /gene="acpT"
                     /locus_tag="ACIYSB_003643"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGC5430600.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6496..7545)
                     /locus_tag="ACIYSB_003644"
     CDS             complement(6496..7545)
                     /locus_tag="ACIYSB_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGC5430601.1"
                     /translation="METPQPDKTGMHILLKLVSLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7677..8894
                     /locus_tag="ACIYSB_003645"
     CDS             7677..8894
                     /locus_tag="ACIYSB_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5430602.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADSLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8898..9455)
                     /gene="dcrB"
                     /locus_tag="ACIYSB_003646"
     CDS             complement(8898..9455)
                     /gene="dcrB"
                     /locus_tag="ACIYSB_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGC5430603.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9528..10193)
                     /locus_tag="ACIYSB_003647"
     CDS             complement(9528..10193)
                     /locus_tag="ACIYSB_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGC5430604.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10414..10659
                     /gene="tusA"
                     /locus_tag="ACIYSB_003648"
     CDS             10414..10659
                     /gene="tusA"
                     /locus_tag="ACIYSB_003648"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGC5430605.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACIYSB_003649"
     CDS             complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACIYSB_003649"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGC5430606.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     ALQKAGYSLRDEQAAEEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRKGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFTGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13033..13659)
                     /locus_tag="ACIYSB_003650"
     CDS             complement(13033..13659)
                     /locus_tag="ACIYSB_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGC5430607.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13800..14159
                     /locus_tag="ACIYSB_003651"
     CDS             13800..14159
                     /locus_tag="ACIYSB_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGC5430608.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSIRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14162..14431)
                     /locus_tag="ACIYSB_003652"
     CDS             complement(14162..14431)
                     /locus_tag="ACIYSB_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGC5430609.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLAWQKKFKVKK"
     gene            complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACIYSB_003653"
     CDS             complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACIYSB_003653"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGC5430610.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15167..16663
                     /gene="ftsY"
                     /locus_tag="ACIYSB_003654"
     CDS             15167..16663
                     /gene="ftsY"
                     /locus_tag="ACIYSB_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGC5430611.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16666..17334
                     /gene="ftsE"
                     /locus_tag="ACIYSB_003655"
     CDS             16666..17334
                     /gene="ftsE"
                     /locus_tag="ACIYSB_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGC5430612.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17327..18385
                     /gene="ftsX"
                     /locus_tag="ACIYSB_003656"
     CDS             17327..18385
                     /gene="ftsX"
                     /locus_tag="ACIYSB_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGC5430613.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18630..19484
                     /gene="rpoH"
                     /locus_tag="ACIYSB_003657"
     CDS             18630..19484
                     /gene="rpoH"
                     /locus_tag="ACIYSB_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGC5430614.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19755..20858
                     /gene="livJ"
                     /locus_tag="ACIYSB_003658"
     CDS             19755..20858
                     /gene="livJ"
                     /locus_tag="ACIYSB_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGC5430615.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21057..21440)
                     /gene="panM"
                     /locus_tag="ACIYSB_003659"
     CDS             complement(21057..21440)
                     /gene="panM"
                     /locus_tag="ACIYSB_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGC5430616.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5105 a         5691 c         5973 g         4767 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg
       61 agcaggggat tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatacg taccccggct gaaccctgaa caatgctacc gccatactgc
      181 gtcatgtcac cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttctattatt
      241 ttaatgacag tatcttcata cgaaaaagcg aagaaaaact tgtggtcaca atgaattgaa
      301 gatttttagg aatatattta gaacgttaca aaagagaagg ttcggatagg gtatttagag
      361 aggaatcagg tgtgctagtg gccctttgtt gatcgttcac caaaggaccc aataatagac
      421 tcaatcttcc ttcggcaagc actgcaaatg cccgtgccgc acgccgcgct gggcgataac
      481 gtcatcggca aaatgctgca cgtcacccat gtcacctttc aacacggcga tttccagaca
      541 gtcgtcgtgg ttgatgtgca catgcagcgt ggcgacggag aggtcgtggt gatgatgctg
      601 ggtggagaca atgcggctgg ctaagtcgcg tttttcgtgt tcatacacat acgacagcac
      661 cgcgaaacct tgcgtgccgt gctgctgggt ggcctcttgc gccagggcgc tacgcagaat
      721 gtcgcggata gcttcggaac ggttgttata accacgacgc tggctcaggc tgtccagcgt
      781 ctccagtaaa tcgtcatcaa gcgtgatggt gactcgttgc atttgcgtta aaccttttct
      841 gtggtgcgac ggcgcacggg gaatgcgggt aataccgcgt tttgtagcac acgtccggcg
      901 tcagaggaaa aggttaattt ctctcccacc acctgggttt cgacgatttg tccgttgtcc
      961 ataaccatta cccgctggca aaagcgttct accaggcgta agtcgtgggt gatgaacagg
     1021 caggcggtgc caaactgttg ttgtagcttt ttcagcaggc gaatgacacc cgcctgtaac
     1081 acgagatcaa ggttagaaac ggcttcatcc agaatcagta gtttcggttc gaccgccagc
     1141 gcgcgagcca ggcagacgcg ctggagctgg ccgccgctta actgcggtgg gcgtttgtcg
     1201 agaacgctgt catcgagatc gaccgccttc agcatttcgc tggcgcgcgc cagttgttcg
     1261 gattttttca gtgatagcag gtggcgcatc ggttcacgca ggatctcgcg cacggttttg
     1321 cgcggattca cggcgctgat ggagtcctga aataccatct gaatatcgcg gcggaacgct
     1381 ttacgctggg cgcgattgag tttcgccagc ggttcgccac gccagctaat attcccctgc
     1441 gcgggcgatt ctaaacccac cagcaaccgc gcgagggtac ttttcccgca gccgctgcgc
     1501 cccagcaggg cgacggtttc gccgcttttc agggtcaggg aaacgttatt cagcaccgcc
     1561 tgatgttgat gttttccgtt aaatccaccg tgcgcatagt gatgggaaag gccggagatg
     1621 ttaagtaaag tcatgatgcc agctccatac cgtagagggc gagatgagcg gaaaccaggc
     1681 tgcgcgtcac tgtatgtttg ggggcgttaa acagcgtttc tacatcgccc tgttcgacaa
     1741 tcttaccgtc agacatcacc gccacgtcat ccgccagacg cgccacaacg cccatatcat
     1801 gggtcaccag cagcattccc ggcgcttgtt tttgcataat gctttccagc agatcgagga
     1861 tgcgcgcctg tgctaccacg tcaaggtcgg tggtcggttc atcggcgatg ataaacggtg
     1921 attcacacag caccgccatc gcaatcatca tgcgctgcaa catgccgccg ctcatctcga
     1981 acgggtacag cttcagcacg cgcgcggcgt tttccagccc caccgcttct atggcagcgg
     2041 taagcgtagc gtcatcggcg ggtttcccta acgccaggca ggtttcccgc gcgtgggtgt
     2101 gcatggtgtg cagtggatta aaggcgctgc gcgggttctg catgatggtg gcaattttga
     2161 tgccgcgcag ggcgcaaggc gaaaccggtt tgccatcggc taaaatttcc cccgccgtct
     2221 ggcgaacgcc agcgggcaaa atgcccagcg tcgcggcgca ggttaatgat ttcccgctgc
     2281 cgctaccgcc gactaacgcc agcacgcgcc cgcgttgcag ggttaacgat acaccgtgta
     2341 ccagcggctg cgcggcctgt agcgcgatat tacgtagttc aatctgttgc ggcattagtg
     2401 tgcgtgctcc gtcaccagat gagggtccag atgatcgcgc agtgcgtcac ccaccaggtt
     2461 aaaggccatc acgctgataa acagcgccag ccccggccag aacatttgca gcggctgggt
     2521 ccagatatac tggcgcgcgt cgttaatcat cacgccccat tcggcggtcg gcgcggtcac
     2581 accgaggcca aggaaagaca tccccgcgac gtgcagcatc atatggccga tatccagcgt
     2641 tgccagcacc agcagcgaag ggatcaccgc gcctgccaga tgatcgacaa acacccgcac
     2701 atggcccgca ccggaaagcc gtgacgccag cacaaactcg cgttggcgta gtgaaatcac
     2761 caggctgcgc accatgcgtg cataccacgc ccagtgcgac agggcgatgg cgataattac
     2821 gttggtcagc ccggtgccga gcacgccaac cataaagaac gacagaatcg aggtcgggaa
     2881 ggtcataaac atatcggcga cgcgcatggt ggcctgatca acgcgcccgc caatcaaccc
     2941 ggcgctgccg ccaataacca gccctaatgt cagcaccagc agcaggcagg ccattaccga
     3001 accgagcgac acgcgggtcg ctgccatcag ccgcgagaaa atatcgcgac ctaagtgatc
     3061 ggtgcccagc cagtgctgcg catccggcga aagcaggcgc gacggcaaat caatcgcctg
     3121 ggggtcatac ggcagccacc actggctggt gagcgcaatc agcgccagca gggcgataat
     3181 gatcagcgcc aggcgtaccg accagcggga agagaggaaa aagttcacgc gtgcgctcct
     3241 tcatgacgac gaatgcgcgg gtccagcgcg gcgttgagca aatcgacaat caaattacag
     3301 accacaaaaa ccaccaccat catcagcgta aagcactgga tcaccggata gtcacggtta
     3361 aaaatcgccg acaccgcata gcgcccgacg cccggccagg caaagatgtt ttcgataatc
     3421 atcgtcccgc caatcagttc gccgatatgc atccccacgg cggtgatcat cggcagcgag
     3481 gcattgcgca ggatgtggcg acgttcggtc tgtttgtcgt tcaggccgcg cagacgcgcc
     3541 caggtgacgt gacgctgacc ggcgacgtcc agcatactgg cgcgcagtaa acgcgcgtta
     3601 atcgccagcg acataaaggc aatggaaacc gcaggcaaaa tgatgtgctg ccagccgccg
     3661 taacccatcg cgggtagcca ttgcagatac accgaaaacg ccatcaccag taaaaacgcc
     3721 agccagaagt taggcatcga cacgccaaga aacgcgatga aacgtacggc gaaatccggc
     3781 agacggtcgc gatggcgcgc cgcccagata ccgagcggta cggaagtgag cagaattaat
     3841 accagcgccg cacctgcaag ttccagcgtg gcgggcagga agttcagcat atcgtccagt
     3901 accgggcgtt ggctggcgaa tgagatacca aagtcaagat gcagcgcctt ccacaaccag
     3961 gtgccgtact ggacgtacag cggctgatcc agtcccagca tggtgcgggt agaggccagc
     4021 atctccggcg tcggcggcag gttagacaga cgcaaataat cgagcgccgg gtcgccggta
     4081 ccgaggcgca gcatcagaaa aatgatcacc gaggcggcga gcaccatcgg gatcagcagc
     4141 agaaagcggc gtaatacgta acgcaacatt aaggtttcac cggtttaatc tgttcgaacg
     4201 gaatttcggt ggcgatcggc gcgtagggga tgttacccag ctccggtttt gataccacca
     4261 tcattgagat gtaactgata ggcagataaa ccgcctcgtc atgcagacgg gtcagaatgt
     4321 cgcgatacag cgcctgacgt tgcgtttcgt catgggtcgc cagcacttcg ccgatctctt
     4381 tatcaatcag cggtttgtcg gctaatcctt gctgtgcctg gaagtcagcg tgtgacggta
     4441 cgcgcattga actgaggaag gcgtgtggat catatggcgc gccccaggtg cggtggaaaa
     4501 tcatgccaaa acgaccgtcg cgctgacgag catagatact gctctcttct tcgccaatca
     4561 gcgagacatc tgcgccaatc tggcgcatat cagcctgaat gatttccgcc atcgatttgc
     4621 ttaacgcatc ggtgccgatg aacgaaagtt caatgcgcag cggctgacca tttttctcgc
     4681 ggatgtcttt gcccgcaggc agcgtccaac cggctttttc cagcaacgct ttcgcttttt
     4741 gcggatcgta ctggctcggt ttcaggccga ggttggcgta gggcacagaa ggggcaaaca
     4801 gggtgtcggc gacctgctgg gtgccataca acgcgttatc aatcagcgat tttttgttta
     4861 ccgcgtaatt aagagcttca cgtactgcca gctcgttggt gggggctttg gcggtattga
     4921 gcgccagcat cacggtttcg atcggctgtg acagttgggt gtggtaagcc ggattctggc
     4981 taaagcgggc gaaggtatcg agcggtaata acccttcgtt tccgtacagc aggtcgatat
     5041 cgccagtttc aaacgccacc gcgcgggtag tcgggtccgg gatgacgtta aaggtgatct
     5101 ttttaatcgc tggcttttcg ccccagtagt tttcgttacg gacgaagaca tcgtactgat
     5161 tcagtttcga ttcctgcaaa atccacggtc cggtgccaat cggcgcttta attccgttca
     5221 tggtttcatg gtttttaaac tgcgagggag cgataaaacg gaaaggacgg ggcagggcca
     5281 gttcttgcag gaaaggatag taggcgcttt tcagggtaat ttgcagctct gttttactga
     5341 gtgctttaac atcaacaatc tggtttgcca gctccagcca ggcgtgacgt tgacggttat
     5401 cgagcactgc gcggaagttt tctgccgccg cctcggcatc gaacggttca ccgttggaga
     5461 atttcacgtc atcacgcagg gtgaaggtcc aggttttacc atcttctgaa tgagtccagc
     5521 tttttgccag ccacgggatc accgaaccgt ctgcctgata tttcaccaat ggttcataaa
     5581 ccatgctctg ggcgaacatc tggttaggcg tgtaaaggtg cgggtttagt ggcccgacat
     5641 tcaccggcca ggcggtggtg atttcatctg gtgcagcggc atggacgata aaagacgcac
     5701 aagccagcag cgcaaataga gtgcggcgga gtgtggagag catggtaacc ccaatggatt
     5761 aaaatagatg gcggaaataa gtatgacgat tttaagtatt cgtcatactg attacctgtt
     5821 ctggatcaat agatgggcag tcgggtgggc ggatcagtta actgaatcga tccattgcac
     5881 actgtcggcg gtgagcgtaa agggagtagg ggtgcagatc gcaaggctta aattttcaag
     5941 ctgacaatgg ctgactgaca gcgaggagtg ataggtgctg tctacgctga cgatttgcca
     6001 ggcgctgccg ccacgctgtt taacgatggc ttctttgcgc gtccagatgc gccagaacat
     6061 ttccagttgc tgatcaggat gcacggcgtc catctcagcg tgttccccga ggctgaatac
     6121 ggcgttcgcc agccagcgcc agttggcgcg cgggcgaatc acttcgatat cgcagccgac
     6181 ttcgccttca tcactcaaca gcagggcgat atcgtcaccg ctatggctta agttgaacca
     6241 tagcggcatt tccggcgcaa atgcaggttt gccttgttcg ccatagatga tctccggtag
     6301 cggggaaagc gtgtgcgaaa gcaatgcacg ccccgccagc cagcgttcgc gtcgtggacc
     6361 ttgcggtgct tgctcgcgta aacccggtgg cagtggagct gcgcttaagg tcgaaacttt
     6421 ccccagaact atccgataca tatcagggcc aacgtttaat ggaaaatgaa agtgcgtatc
     6481 gtatcacttg tcgcctcatc ccggtaaccg acttttcggt ctgcccggcc ccagtaaaat
     6541 cgccagtttg ctaccgcctt tggtggtttc catccagatt ttacacacgc tggttaatgg
     6601 caccgaaagt agcatcccta ccgggccgag cagccatccc caaattaaca atgaaagaaa
     6661 taccaccatg gtggacatcc ccaggcgatg gcccatcatc cgtggttcta aaatattgcc
     6721 gatgaccata tggaccacta aaaacaatgc gccgaccaga atacattcgt aaacaccatt
     6781 aaacagcagc acctgaatca ttggcggtac ggcggaaatt accgcgccga tattgggcac
     6841 gtagttgagc aaaaacgcca gtactgccca catcagcgca aactgcaccc ccatcagctc
     6901 cagccccagc cagacgatga cacctgtcca taaactgagt agcgtcttca atgcaagata
     6961 gtgcgaaacg cctttaagtg cgcggtgtaa tcccgcgatg tgaatctgtg gattattcag
     7021 cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac agcataaaaa ctacggtcat
     7081 caccagcaaa agcacgctcg ccattgcccc ggaaagcccg gtcattagcg ctgtggtgaa
     7141 ggtaaccact ttttccgagt ccatccgctg cagcattcgc tccggcgaca tatgcaaatt
     7201 aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc gtcagctcct tattaaactt
     7261 cggcagcata gagataaatt cgttaaacga tgccgccagt acgccgacca gcgcggttag
     7321 tgcgatcagc atcaccacca ctacaatcgt aatggcaacg gggcgttgta ctccccgacg
     7381 aataaaccag gtgacgagcg ggttgaggac gatggcaaaa aacagcgcca gtaacagctg
     7441 cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc actaccagcg agaccagctt
     7501 gagcagaatg tgcatgcccg ttttatcggg ttgaggggtt tccattgggg cttccttgtg
     7561 actttttgta ttaagtgtag cgggagtcac gccagcaata ttctgattct tactgctgat
     7621 tttcacgtca gcacgtggta aaaatgaaac actgttgtaa aaatgtggtg atcctcatgc
     7681 ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct gaatttgcgc attgtctcta
     7741 tagtcatgtt taacttcgcc agctacctca ccatcgggtt gccgctcgct gtattaccgg
     7801 gctatgtcca tgatgtgatg ggctttagcg ccttctgggc aggattggtt atcagcctgc
     7861 aatatttcgc caccttgctg agccgccctc atgccggacg ttacgccgat tcgctgggac
     7921 ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt gagcggtctg gggtatctga
     7981 cggcaggatt aaccgccagt ctgcctgtca tcagcctgtt attactttgc ctggggcgcg
     8041 tcatccttgg gattgggcaa agttttgccg gaacgggatc gaccctatgg ggcgttggcg
     8101 tggttggctc gctgcatatc gggcgggtga tttcgtggaa cggcattgtc acttacgggg
     8161 cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca ctggggcggc ttgcaggcgt
     8221 tagcgttaat cattatgggc gtggcgctgg tggccatttt gttggcgatc ccgcgtccga
     8281 cggtaaaagc cagtaaaggc aaaccgctgc cgtttcgcgc ggtgcttggg cgcgtctggc
     8341 tgtacggtat ggcgctggca ctggcttccg ccggatttgg cgtcatcgcc acctttatca
     8401 cgctgtttta tgacgctaaa ggttgggacg gtgcggcttt cgcgctgacg ctgtttagct
     8461 gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat taaccgtatc ggtggcttaa
     8521 acgtagcgat gatttgcttt agcgttgaga taatcggcct gctactggtt ggcgtggcga
     8581 ctatgccgtg gatggcgaaa atcggcgtct tactggcggg ggccgggttt tcgctggtgt
     8641 tcccggcatt gggtgtagtg gcggtaaaag cggttccgca gcaaaatcag ggggcggcgc
     8701 tggcaactta caccgtattt atggatttat cgcttggcgt gactggacca ctggctgggc
     8761 tggtgatgag ctgggcgggc gtaccggtga tttatctggc ggcggcggga ctggtcgcaa
     8821 tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc ggaacacgtc cctgaggccg
     8881 cctcatcatc ttaaaactta ctgaataacc agcgtattaa tgatgttttc tgcggtggtc
     8941 tgcgcttttt gctgatcgtc agcgggcagc gtaatttgca tggtcagcag ttgattaccc
     9001 acgttaccca gaataacgga agagtacgcc gtctggcctt tcgcggagat aatactgtct
     9061 aactgctgca ttttgtgacc tttcagctca atggctttat tggttaccac ttgcagctgc
     9121 ggatcgcggc tacgttgctg atcttccaga cgcttcgcca gcaccgccag atcttctttc
     9181 ggatcatcgc ccatgatgac gatgactgct ttctgcccgg tggcgtcgga ccagacatgc
     9241 atgttattgg cctgcgttcc cagcttaccg ctctggtcgg tcatatccgc tggcagcgag
     9301 aaacttaact tgccatcaag caggttgacg ggattcccgg tagcgttact ttccgcgacc
     9361 gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg ccgcaagccc cataaccagc
     9421 aggccaattc cgacatattt aaccagattg cgcattgact tcttcctttc gataaacggc
     9481 cataacggct cattcatcca tcttatcaca actctgataa cgaaccttta actcgcctgc
     9541 aaagcgttga tttcggattt atctgccagt cttttcaaca gcatattgag taatacgcca
     9601 tacattggca ggaagaaaac gatactgatt aacactttga aacagtaatc gaccagcgcg
     9661 atttccatcc agtgttcagc cataaaggca tccgggctac gccagaaggc aatgaagaaa
     9721 aaggccagcg tgtcgctgac gttaccgaac agtgtggacg ctgtcggtgc cagccaccag
     9781 cggcgactct gacgcaggcg gttaaaaacg tgcacgtcga ggatttgccc cagcgcgtag
     9841 gccatgaaac tggcggtggc gatacgggcg acaaacaggt tgaagtgggc gagtgcgccg
     9901 aatccctgcc aggaacccat atagaatagc gacgagatga cgtaggagat taataacgca
     9961 gggatcatta ccgcgaagat aatgcgtcgg gccagcggtg cgccaaaaat acgcacggtc
    10021 aggtcggtag caagaaaaat aaacggaaag ctaaacgcgc cccaggtggt atggaaaccc
    10081 aaaatggaga cgggaagctg aaccagatag ttactggagg tgatcaccag cagatgaaat
    10141 aacgataacc agaacaacgc cttatagcgt tgagtttgcg agaaaacgtt catattgtac
    10201 ctttttgatt aaccattggg gtgagggaac ccaatacgta cgacacgtct gttaccttaa
    10261 cataataacg tgcttaccct tttttcgagc cgccgcatga tactgcttta cgttgacaat
    10321 gcaatggtta attttcacgc aatcgttaac ctagtttgct tacggactca cagggcgtaa
    10381 actagcgccg tttttttaag tgatgagaag aaaatgaccg atctcttttc cagccctgac
    10441 cacacactcg acgcgcttgg cctgcgctgc ccggaaccgg tgatgatggt gcgcaaaacc
    10501 gtgcgcaata tgcagcctgg cgaaacgttg ctgattatcg ccgacgatcc ggccactacc
    10561 cgcgatattc ctgggttttg tacctttatg gaacacgaac tggttgctaa agagacggat
    10621 ggactgcctt atcgttattt gattcgtaaa ggcggttgat aggggctgat tggcttcgat
    10681 gccgcctttt cccctcaccc taaccctctc cccagagggg cgaggggacc gatcgcgctc
    10741 aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc ttaacgcatt cgctgtcacc
    10801 agcaccgtcg cccccgtatc tgccagcact gccagccaca acccggtcat ccctaacagc
    10861 gtggtgacga ggaagatccc tttcagcccc agcgcaatag tgatgttctg gcggatattg
    10921 gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc cgcgcaggtg gttatgggtt
    10981 aatgctgcgt cggcggtttc cagcgccacg tctgtgccgc tacccattgc aatcccgatg
    11041 gcggcagctt tcatcgctgg cgcgtcgtta ataccgtcac cgaccatcgc cagcggcgca
    11101 tgttgattca gctcggtcac cgctttgact ttatcttccg gcaacaggcc cgctttaaac
    11161 tccagcccca gctccccggc aattgccgcc gctgcgcgtg gattatcgcc ggtgaggatc
    11221 acccctttga cgcccagcgc gttcagttca ctgatggcag ttgcagcatc ggcgcgcagg
    11281 gtatcctgta acgcaatgac accaagcacg tcatcgttac gtactaccag cactaccgtt
    11341 tgcccggcgc tttccagttc gttaattaaa ccagtaaatg catcagcggg atgtttcccg
    11401 gcagcgcaaa tcaatacgcg ctcaccgtta acctgcgctt caatgccaga cccgaccagc
    11461 gcccgctgtg attcggcggt gggaatggcg agttcagcaa cctgtgcttc gcgtacgatg
    11521 gcttgcgcca gtggatgcgt cgcgccttgc tcgaccgccg ccgccagtgt cagcagttca
    11581 gattcactaa tacccgttgc cggatgaatc gcggtaacgc gcggtttacc gacggtcagc
    11641 gtaccggttt tatcaaacgc cacctgagta acacgaccca gctgttccag cgccgctccg
    11701 cctttaatca acgccccacg acgcgctgcc gccgccagcc cggaggtaat cgccgcaggc
    11761 gttgagataa ctaacgcaca cgggcagcca atcagcagca gcgtcagccc tttataaatc
    11821 cactcctgcc agctggcggc aaacagcagc ggtggcacca gcgtcaccag cagagcgacg
    11881 gccataatcg cgggcgtata gatacggctg aaacggtcga taaaccgctc aatgggagcg
    11941 cgacgctctt cggcttcttc gatcagtttc agaatccggt caatggcgct ggctcccggt
    12001 tctgacagca cttccaacgt caccagacgg tctacgctgg tggcaccagc agggacttta
    12061 tcgcccgttg cgcgctccac cggaatggat tcgccggtca gggcgctttc atcaaaactg
    12121 gcaaacggtg agagcagttt accgtcggca ggcaaacgcc cacctgcggc gacttcaatc
    12181 acatcgccag ggcgcaggct gttaatcgcc acctcttccc gctcaccctt acgcaggcgc
    12241 gtggcggttt ctggtttcag cgccattaac gcgctaacgc cctgacgcgc gcggctggcg
    12301 gcccagcctt ccagtcgttc accaatcaaa aacagcagca acaccatcgc agcttcagcc
    12361 gttgcgccaa taaacagtgc accaatagcg gctacgctca ttaaggtttc aatggcgaag
    12421 tagctgccgg atttgatcaa ccgtaatgcc tgacgagcaa tcgggtacag cccaaccagc
    12481 gtggtcgcga taaacgccag ttgcccgaac ggatgattga actgctccag accccagctg
    12541 attgccatca tcacgattag cgtaatcagc ggcagattct ctttcaggcg tgatgcttgc
    12601 ggttcttcgg cggcctgttc atcgcgcagg gaatagcctg ctttttgcag cgcagattca
    12661 acttgtgcac gaatgtcatt gtcggcatcg accaccagtt tttcggtggc gaacaacacc
    12721 tgcacctgat tcacgcctgc aagctggcgc acggcatttt ctaccttgcg cgcacaggcg
    12781 gcgcagtcca tgccgctgac tttccagcta tagcgggtgc cggagacgtt ttcagagaga
    12841 gttggcgtgc tggaacatgc gccgtcgcag caacagtcgt tggcgttctg taccgtggtt
    12901 agcggtttga acgcagcaaa ttgaggggct ttcttgccgt gattgtcagg agtcgacatg
    12961 gcatcctccg gttaagtttt ttctcattaa ccgaaggata cactctggag tcgactccag
    13021 agtcaagttt tatcagagat acagcgagcg gacgatcagg aagtgaccgg caaagtagca
    13081 ggccgcagca atcgcgttat ccgcacggaa gcgtcggcga tagtggctcc ccagccagac
    13141 aaagttactg ataaacagca acgaagcgcc aacaaacgca gagagcgccg gagcggtcgg
    13201 acggaagaac cacagttcac ctgccagcca caccatcacc agcgtcatgc cgataaacgt
    13261 gcagataggc caacggtact cttccaggcg cgtccagata atcgccagta acagcgcacc
    13321 cagaaccagc agcaccagtg gtagaggcca gaagaaagag agcgtcatct gactggcgaa
    13381 atagatggtg tacagcaggt gcgagaggaa aaacgcgccg atggcgtaca tcagacgttg
    13441 acgtggcaac agggttagcg catcgcccag cagtgaggcg cacagccctg ccagcaccag
    13501 atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt aacagcagga gaagaagggt
    13561 taacggttta aacacccagc gttgccaggt tggcccacga tacgatgcat ccacagatag
    13621 ccatgcggaa agacagacag cgataaacga ccaaagcatc ttagttcctt gaattcgtta
    13681 ttttttcgta agcggaatgc ctacggtctt gcttccagtt tagtggcgag ggtggacgga
    13741 tgacaacacc ataactggca gggtatgctt atttccgcat tttccgatga gggataaaga
    13801 tgagcaaacc acctcttttc tttattgtta tcattggctt aattgtcgtc gccgcatcgt
    13861 ttcgttttat gcaacagcga cgggaaaaag ctgataatga tatggctccg ctccagcaaa
    13921 agctggtggt ggtgagcaac aagcgggaaa aaccgattaa cgatcgccgt tcgcgccagc
    13981 aggaagtgac tccggcaggc accagtatac gctatgaggc aagcttcaaa ccgcaaagcg
    14041 gaggaatgga gcagacgttt cgcctcgacg cccagcagta ccacgccctg acagtgggcg
    14101 ataaaggtac gctgagctat aaaggaacgc gctttgtcag ctttgtaggc gaacaataag
    14161 gttatttttt aactttaaat ttcttctgcc aggccagcag ttcaaacacg ccgaaaagga
    14221 aaatccgtac cttttcggcg gtggtcatct gcgggccatc tttcggtaaa gtggatttca
    14281 atagcgccag ctgcatacca tgcatcagca cggtaaaaat cagcgccacg ttaacgaaga
    14341 tattcagcgg gcgtgggaag ggatgcacca ggttgaggat taaaaatccc caaacgcaga
    14401 gcattaacaa acgaccaata ttaatcagca tcactttctc cttgtgcttc gcgttgatac
    14461 agccgataag ccacctgacc cgccactttt tcccgatgta atgaccagtt tgctggaaca
    14521 gtgggcagac cgttttcgac ttcgctttcg acataaatca gggcttcgtc agccagccag
    14581 ccgttatctt ccagtaaatt tatcgtctct tctaacaagc cacggcggaa cggtggatcg
    14641 acaaacacga tattatgcgg tgtacctttt tgcgccagga atgacatcgc gttgctgttc
    14701 accacgcgtg cattgcctgc ttttagtgtc gccagattct taattaactg ctgagaaacc
    14761 gcgcgatcca tctcaatcaa cgttgccccc gcagcatagc gcgacaacgc ttccagcccc
    14821 agcgcgccgc ttccggcgaa gcaatccaga cattgggcgt caacaatgac cggagccagc
    14881 cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc gcagacctgg gctatcagga
    14941 accgggagtt tacggcctcg ccactgcccg ccaataatgc ggatttggcc gctgccggaa
    15001 tgattcggtt ttttcatgat aaattgctca atccgccaga taacacatac ttgcaggcgg
    15061 tgatgtgaat taagttaagt gatagactat ttcatcattt ttttagctgc tatgtacata
    15121 gcgttaacgc tgtgccatga agcaacagcg aggagtgtag tcgcaaatgg cgaaagaaaa
    15181 aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa gagcagaccc cggaaaaaga
    15241 gacagaagtt cagaatgaac aaccggttgt agaagaaatc gttcaggcgc aagagcctgt
    15301 gaaggcttcg gaacaagccg ttgaagagca gccgcaggcg catactgaag ccgaggcgga
    15361 aacttttgct gccgacgttg tggaagtcac tgaacaggtt gctgaaagtg aaaaagcgca
    15421 gcctgaagcg gaagtcgttg cacagccgga accggtcgca gaagaaacgc cggagccagt
    15481 ggctatcgaa cgtgaagagc tgccgttgcc agaagacgtc aacgccgaag aggtttcgcc
    15541 agaagagtgg caggctgaag cggaaaccgt agagattgtc gaagcggcag aagaagaagc
    15601 ggctaaagaa gaaatcaccg acgaagagct ggaagcacag gcgctggctg ccgaagcggc
    15661 agaagaggcg gtgatggtag ttcctccggc agaagaagag cagccggtgg aagaaattgc
    15721 ccaggagcaa gaaaaaccga ccaaagaagg tttcttcgcg cgcctgaaac gcagcctgtt
    15781 aaaaaccaaa gaaaatctcg gttccggatt tatcagcctg ttccgcggta aaaaaatcga
    15841 cgatgatctg tttgaggagc tggaagaaca gctgttgatc gccgatgtgg gcgtggaaac
    15901 cacacgtaaa attatcacca atctgacgga aggcgcatcc cgcaagcagc ttcgtgatgc
    15961 cgaggcgctc tatggcctgc tgaaagaaga gatgggcgag attctggcga aagtcgatga
    16021 gccgctgaat gttgaaggta aaacgccctt cgtgatcctg atggtgggcg tcaacggtgt
    16081 gggtaaaact acgacgattg gtaagctggc acgtcagttt gagcagcagg gtaaatcggt
    16141 gatgctggcg gcgggcgata ctttccgtgc agcagcggtt gaacagcttc aggtctgggg
    16201 tcagcgcaac aatattccgg tgattgccca gcataccggt gcggattccg cctctgttat
    16261 cttcgacgcc attcaggcag ctaaagcgcg taatatcgac gtcctgattg ccgataccgc
    16321 cggacgcctg cagaacaaat cgcacctgat ggaagagttg aagaaaatcg tccgcgtgat
    16381 gaagaaactc gacgttgaag cgccgcatga agttatgctg actattgatg ccagtaccgg
    16441 gcagaatgcg gtaagccagg ccaaactgtt ccacgaagcc gttggcttaa ccggcatcac
    16501 gctaacgaaa ctggacggca cggcgaaagg cggggtaatt ttctcggtgg ctgaccagtt
    16561 tggtatccct atccgctaca ttggtgtcgg cgaacgtatt gaggatttgc gtccgtttaa
    16621 ggcggacgac tttatagagg cactttttgc ccgagaggat taacaatgat tcgctttgaa
    16681 catgtcagca aggcttatct cggtgggaga caggcgctgc agggcgttac gttccatatg
    16741 cagccgggtg agatggcgtt tctgaccggt cattccggcg cagggaaaag taccctcctg
    16801 aagctgatct gtgggattga gcggcccagc gccgggaaaa tctggtttag cggccatgac
    16861 atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc gccagattgg catgattttc
    16921 caggatcacc atctactgat ggaccgtact gtctacgata acgtggcgat cccgctgatt
    16981 atcgctggtg ccagcggtga cgatattcgt cgccgggtgt cggcggcact ggataaagtc
    17041 gggctactgg acaaagcgaa gaacttccct attcagcttt ccggcggtga acaacagcgt
    17101 gttggcattg cccgcgcggt ggtgaataag cccgcggtac tgctggcgga cgaaccgacc
    17161 ggtaacctgg acgacgcgtt gtcggaaggg attctacgtc tgtttgaaga gtttaaccgc
    17221 gttggggtaa ccgtattgat ggcaacgcac gacatcaacc tgatttcacg gcgttcctat
    17281 cgcatgctca ccctgagcga tggtcacttg catggaggcg tgggccatga ataagcgcga
    17341 tgcaatcaat catattcggc agtttggcgg gcgtcttgat cgcttccgta aatcggtcgg
    17401 cggctctggc gacggcggtc gtaacgcccc gaaacgcgcg aaatcctcgc caaaaccggt
    17461 aaatcgtaaa accaacgttt tcaacgaaca ggtgcgctat gccttccacg gcgcattgca
    17521 ggatctgaaa agcaaaccgt tcgccacgtt tttaacggtg atggttatcg ccatctccct
    17581 gacgctgccc agcgtctgtt atatggtgta caaaaacgtt aatcaggcgg cgacgcagta
    17641 ttatccgtca ccgcaaatca ctgtttactt gcaaaaaacg ctggacgatg acgccgctgc
    17701 gggcgtggtg gcacagttgc aggccgagca aggcgtggag aaagtgaact atctttctcg
    17761 tgaagacgca ctgggcgagt tccgtaactg gtctggtttt ggtggtgcgc tggatatgct
    17821 ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg aaactcgatt ttcaggggac
    17881 ggaatcactc aatacgctgc gtgatcgtat cacgcagatt aacggcattg acgaagtgcg
    17941 gatggatgac agctggtttg cccgtctggc ggcgttgacc gggctggtcg ggcgcgtttc
    18001 ggcgatgatc ggcgtgttga tggtggcggc agtgttcctc gtcatcggca acagcgtacg
    18061 tctgagcatc tttgctcgcc gtgactccat caacgtgcag aaactgattg gtgcgacgga
    18121 tggtttcatc cttcgcccgt tcctgtatgg tggcgcactg ctgggatttt ctggcgcatt
    18181 gttgtcatta attttgtcag aaattctggt gctgcgattg tcatcggcgg ttgcggaagt
    18241 ggcacaggtt ttcggaacga agtttgatat caatggttta tcgttcgatg aatgcctgct
    18301 attgctgctg gtatgctcga tgattggctg ggtggcagcg tggcttgcca cggtacaaca
    18361 tttacgccac tttacgcctg aataataaaa gcgtgttata ctctttccct gcaatgggtt
    18421 ccgtagcagg gaaagagatc ccgttgtctc ttcccggtat ttcatctcta tgtcacattt
    18481 tgtgcgtaat ttattcacaa gcttgcattg aacttgtgga taaaatcacg gtctgataaa
    18541 acagtgaatg ataacctcgt tgctcttaag ctctggcaca gttgttgcta ccactgaagc
    18601 gccagaagat atcgattgag aggatttgaa tgactgacaa aatgcaaagt ttagctttag
    18661 ccccagttgg caacctggat tcctacatcc gggcagctaa cgcgtggccg atgttgtcgg
    18721 ctgacgagga gcgggcgctg gctgaaaagc tgcattacca tggcgatctg gaagcagcta
    18781 aaacgctgat cctgtctcac ctgcggtttg ttgttcatat tgctcgtaat tatgcgggct
    18841 atggcctgcc acaggcggat ttgattcagg aaggtaacat cggcctgatg aaagcagtgc
    18901 gccgtttcaa cccggaagtg ggtgtgcgcc tggtctcctt cgccgttcac tggatcaaag
    18961 cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt caaagttgcg accaccaaag
    19021 cgcagcgcaa actgttcttc aacctgcgta aaaccaagca gcgtctgggc tggtttaacc
    19081 aggatgaagt cgaaatggtg gcccgtgaac tgggcgtaac cagcaaagac gttcgtgaga
    19141 tggaatcacg tatggcggca caggacatga cctttgacct gtcttccgac gacgattccg
    19201 acagccagcc gatggcaccg gtgctctatc tgcaggataa atcatctaac tttgccgacg
    19261 gcatcgaaga tgataactgg gaagagcagg cggcaaaccg tctgaccgac gcgatgcagg
    19321 gtctggacga acgcagccag gacatcatcc gtgcgcgctg gctggacgaa gacaacaagt
    19381 ccacgttgca ggaactggct gaccgttacg gtgtttccgc tgaacgtgtg cgccaactgg
    19441 aaaagaacgc gatgaaaaaa cttcgcgccg ctattgaagc gtaatttccg ctattaagca
    19501 gagaaccctg gatgagagtc cggggttttt gttttttggg cctctacaat aatcaattcc
    19561 ccctccggca aaacgtcaat ccccacgcag attgttaata aactgtcaaa atagctattc
    19621 caatatcata aaaatggggt atgttttagc agagtatgct gctaaagcac gggtagtcat
    19681 gcataaaacg aaataaagtg ctgaatcaca acaccacaac acacgtaata accagaagaa
    19741 tggggattct caggatgaat ataaagggta aagcgttact ggcaggatgt atcgcgctgg
    19801 cattcagcaa tatggctctg gcagaagata ttaaagtcgc ggtcgtgggc gcaatgtccg
    19861 gtccggttgc gcagtacggt gaccaggagt ttaccggcgc agagcaggcg gttgcggata
    19921 tcaacgctaa aggcggcatt aaaggcaaca aactgcaaat cgtaaaatat gacgatgcct
    19981 gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt taacgacggc attaaatatg
    20041 tgattggtca cctctgttct tcatcaacgc agcctgcgtc tgatatctac gaagacgaag
    20101 gcattttaat gatcacccca gcggcaaccg cgccggagct gaccgcccgt ggctatcagc
    20161 tgatcctgcg caccaccggc ctggactccg accaggggcc gacggcggcg aaatatattc
    20221 ttgagaaagt gaaaccgcag cgtattgcta tcgttcacga caaacagcaa tacggcgaag
    20281 gtctggcacg agcggtgcag gacggcctga agaaaggcaa tgctaacgtg gtgttctttg
    20341 atggtatcac cgccggggaa aaagatttct ctacgctggt ggcgcgtctg aagaaagaga
    20401 atatcgactt cgtttactac ggcggttacc acccggaaat ggggcaaatc ctgcgtcagg
    20461 cacgcgcggc agggctgaaa actcagttta tggggccgga aggtgtggct aacgtttcgc
    20521 tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt gaccaagccg aagaactacg
    20581 atcaggttcc ggcaaacaaa cccattgttg acgcgatcaa agcgaaaaaa caggacccaa
    20641 gcggcgcatt cgtttggacc acctacgccg cgctgcaatc tttgcaggcg ggcctgaatc
    20701 agtctgacga tccggctgag atcgccaaat acctgaaagc gaactccgtg gataccgtaa
    20761 tgggaccgct gacgtgggat gagaaaggcg atctgaaagg ctttgagttc ggcgtatttg
    20821 actggcacgc caacggcacg gcgaccgatg cgaagtaatc attaatcggc aactttgggt
    20881 tgccgccaaa ttgctaatat cgagtacgtt gcttcatgcc ggatgcggcg taaacgcctt
    20941 atccggccta caagatccaa agaaatcagt aaattgcaac acacattgta ggcctgataa
    21001 gcgtagcgca tcaggcaatt tctcgttggt catcactttt gaaatcaact tgacgattaa
    21061 cgcttctccc agccgccctg ttgcgccgta aaccccagcg cctgcataaa cgccgtcatc
    21121 acaccgcgat cttccaagcc tgcatccgcc atccaccagc atgaaacgcc aggattgtta
    21181 cgcaaaacct cttccagcag atattgcccc acaccgcgac gtcgggtgac ttcccgcaca
    21241 cgcagggaat ccagtgctcc ctcggtgccg cttaaggtta cccgtacggc agcgagcagg
    21301 cgctcgttaa aacgcgcggc gtagatacgg tggttatcgt caacctgtaa agaggaaggg
    21361 gaatactccg gccagatctt ttgcaggtca atccggtcct ggtcgctaaa gttttctaat
    21421 cgaatgatgg tcagcttcat gggtaacccg tgtaaatcac aaaagtggaa ccagtgtagc
    21481 gaaataattt aatcggaggc tttctctttt ttatttcttt tggcaggtga ttaatt
//
LOCUS       DBBGLQ010000074        21268 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.074, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000074 DBBGLQ010000000
VERSION     DBBGLQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.074"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..1260
                     /gene="espX5"
                     /locus_tag="ACIYSB_003660"
     CDS             <1..1260
                     /gene="espX5"
                     /locus_tag="ACIYSB_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HGC5430617.1"
                     /translation="PLCLINDNHSVTSPSHTKKTKSDNYSKHHKNTLIDNKALSLFKM
                     DDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIASHANNKCDDRITRRVYL
                     NKDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYDFSHLSLKGIVIKDEDLSNSNFAG
                     CRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDDSNFAQIKMTDGTLNSCSAMHVQF
                     YNATMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYINLFRADLSFSKLDLINFEHA
                     DLSRVNLNKATLQNINLIDSKLFFTRLTNTFLEMVICTGSNMANVNFNNANLSNCHFN
                     CSVLTKAWMFNIRLYRVNFDEASVQGMGITILRGEENISINSDILVTLQKFFEEDCAT
                     HTGMSQTEDNLHAVAMKITADIMQDAD"
     gene            complement(1414..3063)
                     /locus_tag="ACIYSB_003661"
     CDS             complement(1414..3063)
                     /locus_tag="ACIYSB_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGC5430618.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(3214..4563)
                     /gene="ghxP"
                     /locus_tag="ACIYSB_003662"
                     /gene_synonym="yjcD"
     CDS             complement(3214..4563)
                     /gene="ghxP"
                     /locus_tag="ACIYSB_003662"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGC5430619.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(5109..5573)
                     /gene="soxR"
                     /locus_tag="ACIYSB_003663"
     CDS             complement(5109..5573)
                     /gene="soxR"
                     /locus_tag="ACIYSB_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGC5430620.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5659..5982
                     /gene="soxS"
                     /locus_tag="ACIYSB_003664"
     CDS             5659..5982
                     /gene="soxS"
                     /locus_tag="ACIYSB_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGC5430621.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5985..7571)
                     /gene="pdeC"
                     /locus_tag="ACIYSB_003665"
     CDS             complement(5985..7571)
                     /gene="pdeC"
                     /locus_tag="ACIYSB_003665"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGC5430622.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            8001..8282
                     /locus_tag="ACIYSB_003666"
     CDS             8001..8282
                     /locus_tag="ACIYSB_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGC5430623.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(8381..8917)
                     /gene="ssb1"
                     /locus_tag="ACIYSB_003667"
     CDS             complement(8381..8917)
                     /gene="ssb1"
                     /locus_tag="ACIYSB_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGC5430624.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            9171..11993
                     /gene="uvrA"
                     /locus_tag="ACIYSB_003668"
     CDS             9171..11993
                     /gene="uvrA"
                     /locus_tag="ACIYSB_003668"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGC5430625.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(12028..12384)
                     /locus_tag="ACIYSB_003669"
     CDS             complement(12028..12384)
                     /locus_tag="ACIYSB_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGC5430626.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12388..12804)
                     /locus_tag="ACIYSB_003670"
     CDS             complement(12388..12804)
                     /locus_tag="ACIYSB_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGC5430627.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12915..13628)
                     /gene="aphA"
                     /locus_tag="ACIYSB_003671"
     CDS             complement(12915..13628)
                     /gene="aphA"
                     /locus_tag="ACIYSB_003671"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGC5430628.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            14030..14233
                     /locus_tag="ACIYSB_003672"
     CDS             14030..14233
                     /locus_tag="ACIYSB_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbS"
                     /protein_id="HGC5430629.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFFLYIETQLKIAK
                     DFLDLEKKYQVLLQVKFFTHLLI"
     gene            complement(14735..15928)
                     /gene="tyrB"
                     /locus_tag="ACIYSB_003673"
     CDS             complement(14735..15928)
                     /gene="tyrB"
                     /locus_tag="ACIYSB_003673"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGC5430630.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNCYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(16181..17260)
                     /gene="alr"
                     /locus_tag="ACIYSB_003674"
     CDS             complement(16181..17260)
                     /gene="alr"
                     /locus_tag="ACIYSB_003674"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGC5430631.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(17313..18728)
                     /gene="dnaB"
                     /locus_tag="ACIYSB_003675"
     CDS             complement(17313..18728)
                     /gene="dnaB"
                     /locus_tag="ACIYSB_003675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGC5430632.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            18811..19794
                     /locus_tag="ACIYSB_003676"
     CDS             18811..19794
                     /locus_tag="ACIYSB_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGC5430633.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(19960..20202)
                     /gene="pspG"
                     /locus_tag="ACIYSB_003677"
     CDS             complement(19960..20202)
                     /gene="pspG"
                     /locus_tag="ACIYSB_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGC5430634.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(20336..>21268)
                     /gene="dusA"
                     /locus_tag="ACIYSB_003678"
     CDS             complement(20336..>21268)
                     /gene="dusA"
                     /locus_tag="ACIYSB_003678"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGC5430635.1"
                     /translation="WTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEH
                     PVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVAD
                     CVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGL
                     SPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQ
                     NPGILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIP
                     GARQWRRYLSENAHKAGADINVLEHALKLVADKR"
BASE COUNT         5607 a         5473 c         5123 g         5065 t
ORIGIN      
        1 cctctttgcc tgattaacga taaccactct gtcacaagtc catcacatac aaagaaaaca
       61 aaatcagata attacagcaa acatcataaa aacacgttaa ttgacaataa agccctctct
      121 cttttcaaaa tggatgatca tgaaaaagtg ataggcttga ttcagaaaat gaaaagaatt
      181 tatgatagtt taccatcagg aaaaatcacg aaagaaacgg acaggaaaat acataaatat
      241 tttatagata tagcttcaca tgcaaataat aaatgtgacg atagaattac gagaagagtt
      301 taccttaata aagataagga agtgtcaatt aaggtggtat attttataaa taatgtcacc
      361 gtccataata atactatcga aatcccacag acagtaaatg gtggttacga tttttcacac
      421 cttagcctga aaggtatcgt gattaaagat gaagatttat ccaattcgaa ttttgcaggt
      481 tgcagactac aaaacgctat ttttcaggac tgtaatatgt ataaaacgaa ttttaatttc
      541 gccataatgg aaaaaatact ttttgataat tgtattctcg atgactcaaa tttcgctcag
      601 ataaaaatga ctgacggaac tctaaattca tgttccgcta tgcatgttca attctacaat
      661 gcaacaatga atagagccaa tattaaaaat accttccttg attattcaaa tttttatatg
      721 gcatacatgg ctgaggtaaa tctttataaa gtaatagcgc catatattaa tttatttaga
      781 gccgacctta gcttctctaa acttgattta attaactttg aacatgctga tctgtctcgt
      841 gtcaacctga ataaagcaac cctccagaat ataaacttaa ttgatagcaa actctttttt
      901 acgcggttaa caaatacgtt cctcgaaatg gttatatgta ccggctctaa tatggctaat
      961 gttaatttta ataatgccaa tttaagcaat tgccatttca actgttctgt tttaacaaaa
     1021 gcctggatgt ttaatatccg tctctatcgt gttaatttcg atgaggctag cgtccaggga
     1081 atgggtatta ccattctccg tggtgaggaa aatatctcca ttaatagtga tatcctggta
     1141 acactacaga aattctttga agaagattgt gccactcata ctggcatgtc acaaactgag
     1201 gataatcttc atgcagtcgc tatgaagatt actgcagata ttatgcaaga tgcagattga
     1261 ggttaagtcg aagaacagcc aatgaattga ctttaaaaga gagataactt atttgtttta
     1321 gaatcatgaa tgttagcccg agctaacctg ttaaaggtgc aaaagattgc acctttttca
     1381 gattacctcg ggcctggtta cgtcgctttc ggcttactga ttttcctcaa ttagcaacgc
     1441 ttcaagcaaa tcgagatcgt gcagtaattt ttgcagcgtt tcgttgctga tctcccgtgt
     1501 ggcgcgcagg tggtaaagtt cagcacgttc agaacgcaat gccgccagac ggaagcgacg
     1561 ctcaaggttc tcttcctgca cggaactttc aacgtcgtta cgtccatcgg cgcgacgacg
     1621 caggttacca atgacgcgag aactgacctc cgtaagcagc tggttatcga tattctcttc
     1681 ggtatccgcc gccagacgct cctccatttt ctggatcgcc acaatcgcca cttctgccgt
     1741 tgccgctcgc gcaatacgtt cctctttcaa ttgttgcgaa tgatccgcga cttcaatgtg
     1801 ttgtagcaga ataggcaaca tcaccacgcc gacaaacagc gagaagagaa tgacgccagc
     1861 cgccaggaac accagctcat agcgcgccgg gaagacgtta ccatccggca agagcagcgg
     1921 gatagagagc acaccggcca gagtgatcgc cccacgcacc ccggcgaaag acgcgatcag
     1981 gatttctcgt gtggtccacg aaccaaactc catcggcttc ttcttcagga agcggttgct
     2041 gaactttttc atcgtccaca gccagccgaa acggaccagc atcagcgccg catatatcag
     2101 aataatatcg gtaaacagca tccagatttc gacgttaggg tcgatttctg ctgccatcag
     2161 cgacgtctcc agaatacccg gcagctgcag acctaacagc aggaacacca tgccgttaaa
     2221 gacaaattcc agcatcgccc aggtgctgtt tgcacgcagg cgcattgcca gcggcgcacg
     2281 gcgcatcaca ccggagcggg tgatggtcat cccggcggca accgcagcga ggatgccgga
     2341 aacgccaata tgttcggcaa tcagataaga agcgaatggc agcaggaaca gcaggacgat
     2401 ctgcgtcgcg ggttcatcac cgccccagcg gctgaggaat cgcagcgagc gaccgtacag
     2461 ccagctcacc acaaaaccgg cgagaatacc gccaatggct actttcataa attcgaccgt
     2521 cgcaccgccg acggtgaaga tcatcgtccc catcgccact gctacggcaa acttcaacga
     2581 caccagaccg gaggcgtcat tcatcaacgc ttcgccctgc aaaatgccca tgattttttt
     2641 cgggatgcgc ccttcgccaa caatcccgga gagcgccaca gcatcggtcg gagaaagcac
     2701 cgccgccagc gcaaaggcag ggatcagcgg aatgcccggc accacccagt aaataaggaa
     2761 gccaatgccg accacggtga ccaccaccag cgccagcgcg aggccgaaaa tctctcgacc
     2821 atgttcgaga aattcacggg tcggcgtttt ccagccatca gcgaacagca acggcgggat
     2881 aaacaagact aaaaagagtt caggatcaaa ctccacatgc aaaccaaacg tcggccacgc
     2941 cagtagcgca ccgatggcga tttgcataag cggaagcggg atctgaaagg gcatgacacg
     3001 agtgaccacc ccggacagcg agaccacgag ggtcattatc agtatggtga agaagatttc
     3061 catgcgttcc ctgtttgcga tgttttttat gatctgagaa agacgttatg cctgaattct
     3121 cattttttca gaatatcgca aaagctcaca caatgtgcgg agaaaataaa aacgggcagc
     3181 cattgccacc cgttttcaga atccgatgaa agattagata gcccagccac ccgcatagaa
     3241 ggtcaccagc gccacggcga taacgaccgt accgatgttc aacttgcgcc attcgccgga
     3301 aaccagacga ccaatcacca gagtcgcgaa gccgatcatg atgcctgtta cgatgttaca
     3361 ggtcagcacg atgaatacag ccgtaaccag acccgccatc gcatcaacaa aatcagcaaa
     3421 gtcgattttc gccacgttgc tcagcatcag caggccaacg tacatcagcg ccggagccgt
     3481 tgcatacccc ggaacgaggt aagagagcgg agagaggaac agaatcagga ggaacagcac
     3541 gccaacggtg atagccgtca aaccggtttt accgcccgcc gccgtacccg ccgcagactc
     3601 gatgtatacc gctgccggag ccgcacccac caggccagag aaaacgctgc tcatggagtc
     3661 agtggtcagt gctttcccac cgtcgatgat ctgcccatct ttatccagca ggttcgcctg
     3721 gccggcgacg gcacggatag ttccggtggc atcaaatact gccgtcatca ccagcgccag
     3781 aacgcttggc aggactacag gattcagcgc gcccataatg tccaggctgc caatcagtga
     3841 attgccgttt tcatcgctca atgaaggcat ggcgaaaacg ccggagaaat ggacgttagg
     3901 atcgaagatc aaaccgacaa ttgagatacc gataatggtc agcagaatgc caccagggac
     3961 tttcagtttt tccaggccga tgatcaccgc cagacctacc agtgacataa tcaccgggaa
     4021 ggtcgcgaaa tcacccagcg caacgggcag accatcaagc gggtttttaa tcaccagacc
     4081 gacaccgtta gcggcaatga gcagcaggaa cagaccgata ccaatccccg tgccgtgcgc
     4141 cacaccgtga ggcaagttgc gcaaaatcca gctacggata cccgtggcag aaattaccgt
     4201 aaacagcaca cccatcagga acacggcacc cagcgcgaca ggtacgctaa tatgttgccc
     4261 cagcaccagg ctgaatgcgg taaacgctgt cagggagatg gcgcaaccaa tcgccaacgg
     4321 cagattagcc cacagaccca tcacgataga accgagtccg gcaaccagac aggttgcaac
     4381 gaaaactgcc gcaggcggga agcctgcttt acccaacata cctggaacga cgatgaccga
     4441 gtagaccatc gccagaaacg ttgttaaccc ggcaaccact tcctgacgga cagtgcttcc
     4501 acgttgtgaa attttaaacc aggcgtcgag tgaaccgccg gtacgcgctg atggcgtaga
     4561 cataagaaaa catcccctga gagtttaatt ttcgtcaaga tgcgtggcgg acaatcccct
     4621 gccagcgaaa cgttatcttc ctgctccagg tttgcgacaa tttgctgcgt cgcaaaaaag
     4681 caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa aagcaaacga ttatccggcg
     4741 atcatagcgg ctttttcaac cgaagtttgc tgcttttttc tcttttattg tcttgacgat
     4801 gaaaaaccag gcaattgatg cgcaaacgtt atcgtcggtg cgcaagattg cagcgttatc
     4861 gagccgtata ccgttcagga ttgcgataaa aaatcgtctg agatgacaaa aatattgcgg
     4921 ttaaaaagta tacttttaca ctggcgaaga aacgtcggtg acatacaatt aaagcatcaa
     4981 caccaaccgg aacctccacc acgtgctcga atgaggtgtg ttgacgtcgg gggaaaccct
     5041 cctgtgtacc agcgggatag agagaaagac aaagaccgga aaacaaacta aagcgccctt
     5101 gtggcgcttt agttttgttc atcttccagc aagcgtgcgc cggtaccttc ttctcctaag
     5161 cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa ggcagccaca accaatacat
     5221 ccgtccagtt cgtcacgcag cgccactaag gtatgaatgc gccgatccaa ctcttctcgc
     5281 cattgggacg aaagctgttt ccactctttc gcacttaacg tatgcccttc gggcaacacg
     5341 ccaaacgctt caccaatggt cgccagcgga atgccaatac gctgagcaat tttgataatt
     5401 gcaacatatc gcaacacatc acgtttatat cgccgctgat tgccgctgtt acggatactg
     5461 gtaatcaacc ctttactttc atagaaatgc agcgccgata ccgccacacc gctgcgtttc
     5521 gccacttcgc cgggggttag cagcgcttta atgcggggta atttcttttc cataaatcgc
     5581 tttacctcaa gttaacttga ggaattatac tccccaacag atgaattaac gaactgaaca
     5641 ctgaaaagag gcagatttat gtcccatcag aaaattattc aggatcttat cgcatggatt
     5701 gacgagcata ttgaccagcc gcttaacatt gatgtagtcg caaaaaaatc aggctattca
     5761 aagtggtact tgcaacgaat gttccgcacg gtgacgcatc agacgcttgg cgattacatt
     5821 cgccaacgcc gcctgttact ggccgccgtt gagttgcgca ccaccgagcg tccgattttt
     5881 gatatcgcaa tggacctggg ttatgtctcg cagcagacct tctcccgcgt tttccgtcgg
     5941 cagtttgatc gcactcccag cgattatcgc caccgcctgt aattttattg cccgcgcgtt
     6001 aactcccgcg cgggtaattg ctccatccat tgcataaaca cctgcggcgg catcgccttc
     6061 gcaaagaacc atccctggca atagcgcacg ccgcgtttgc gcagccagtt aacctgctcc
     6121 tcagtttcga cgccttcagc gatcgttttt aaccccaggc tgtgcgccag ctcgatgatg
     6181 tgttccgcaa tcaaatgact ggttttgtgg gtggtcagcg tttcaacaaa cgatttgtcg
     6241 attttcaaaa tatcgacatt caatgattta aggttatgca agttagagta gccaatacca
     6301 aaatcatcaa ttgccacttc gtaacctgcc tggcggaaag ccagaataat cggcgtcatt
     6361 ttgtcaacat caagaaatgc atgctcagtc acttcaaatt taatttgctg cggacgcacc
     6421 gcgtattgct ctgttttctg attgattcgc gctatcaacc gtgacgtatg aaaatcggag
     6481 gccgacaggt taatagaaac atagcgatct gcatgtgttg ccaggtaatc gcccagatcg
     6541 cggaagacat tatcaataac ataatcagtt atctgttcta tcatcccctc cttttctgcc
     6601 agcggaataa actctgccgg attcattatt tgcccctgct caccaggcca acgtaacaac
     6661 gcttcagcgc cgatacattt ttctgttttg atatcgatta ttggctggta ataaagacaa
     6721 agttgatgtt tttcgagggc gcgttgcaat ttacgtttgg gagataaata gttttgtcga
     6781 atacgtagcc agagtaatag cagaacaaga ctccccaaaa taccggcggg caacgcaaaa
     6841 ataagatgat tataaaaatg ggttataaga cgttgatatg aagtcgcaac aatggctgca
     6901 attgggcgtt ttgtcgaata aactgtcgca tataaatagc catttctttg tacggttaaa
     6961 tccttcaaat gaatcagcgg agaaaacgtt gctgccgagg cctctttgct taacgagaaa
     7021 aaggttttcg tcaccgtatc atacacaccc cattgcaatg tcgggtcatc agacatcact
     7081 tcactccaga agagagggtt gataaccgcc acataatttc cccgctgcat ataggtcatt
     7141 ttatagccag agaaaaaagg cgtatcgcgg taataataga tagaaacgtt aggttcacgc
     7201 ttataatcgg ccggtgcaat cgtatagccg tttacaggcg ctatcagcga tgagcataaa
     7261 aaatggttat cacgggcata gatcaattca ttaatataaa gatagccacg aataatattc
     7321 aacattcgct tttgatgggc tggagtgcaa acttgcccct gatagcgttc agctgcatcg
     7381 ctcactaaat ctgcctggcg aatgaccagc tcggatttat ccagtgccag ttgagcaaag
     7441 gttcggagct gattattcac ttctgatttt gcccaaagga aggcaatcca tagcgaaaga
     7501 atgatgggaa agagaactaa aaagataatg cccggcaacg ccagtaattg gtgtcgtgca
     7561 cgatgactca ttgctatcaa tttcctgcct gtttataccc ggccattgca aggtgaaaac
     7621 cctgggtacc gacgtcttta gataaatctt acggtataaa tagcactttt gcctgatttg
     7681 aaattgatac ctttctaatc gtcacctgga tgaaaataat tcattgatat tcaaaattaa
     7741 tttcctacag gaatgcagag gcggcgggaa gatatgccct taatgccact gaaaaaccac
     7801 ataatctgta cagcaataaa agtcacggcc taatatggct aacaaacaat caattgtgat
     7861 atagttcaca aaattaatga aacaaacaga gtgtttcatt tttgtgttct gcaagccccg
     7921 cgatagccag agtgtaccac gcctcccgtg aacaacgccg cgctgtccag ggcatcggct
     7981 cttttcagag gaattgtttg atggctaccc tcaccactgg cgtggttctt cttcgctggc
     8041 aacttcttag tgccgtaatg atgtttctgg ccagcacact caacatccgt tttcgtcggt
     8101 ctgattatgt cgggcttgcg gtgatcagca gcggtctggg cgttgtttcc gcctgttggt
     8161 tcgcaatggg gttgcttggc atcacaatgg cggacatcac cgctatctgg cacaacatcg
     8221 agtcggtgat gatagaagag atgaatcaga caccgccaca atggccaatg attttgacct
     8281 gatacgtaga agcctccata acggaggctt cttttttatg gctggcagat gctttaatca
     8341 tccaccttaa aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat
     8401 caaagtccat cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact
     8461 gcgcgccgcc gctgaactga ttgccaccct gcggctgctg aggctgaccc caaccgccct
     8521 gcggctgacc accaccgata ttgccacctg ccggagcgcc accaccctga cgaccaccca
     8581 gcatctgcat ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg
     8641 attgatcggt ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac
     8701 gcagatattc gctcgccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag
     8761 tctgttcttt catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa
     8821 tgttggcaac tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac
     8881 caacgagaat aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc
     8941 gttctgaata agtgtaaacg cgcgattgta ccattaccaa tagcgctttt actatgttgt
     9001 gacctcggtt ccgggaaaca aacctggcca gacattgtta cacaacactc cgggtaatgc
     9061 attccaatac tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc
     9121 cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga
     9181 tcgaagttcg gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg
     9241 acaagctcat tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca
     9301 ccttatatgc cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt
     9361 ttctgtcact gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct
     9421 caattgagca gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa
     9481 tccacgacta tttgcgtttg ttattcgccc gcgttggcga gccgcgctgt ccggaccacg
     9541 acgtcccgct ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg
     9601 aaggcaagcg tctgatgcta ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca
     9661 aaacgctgga gaacctggca agccagggct acatccgtgc tcgtattgat ggcgaagtct
     9721 gcgatctttc cgatccgcca aaactggaac tgcaaaagaa acataccatt gaagtggtgg
     9781 ttgatcgctt caaggtgcgt gacgatctta cccaacgtct tgccgagtca tttgaaaccg
     9841 cgctggagct ttccggtggt accgcggtag tggcggatat ggacgacccg aaagcggaag
     9901 agctgctgtt ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg
     9961 agccgcgact gttttcgttt aacaacccgg cgggggcctg cccgacctgc gacggccttg
    10021 gcgtacagca atatttcgat cctgatcgag tgatccagaa tccggaactg tcgctggctg
    10081 gtggtgcgat ccgtggctgg gatcgccgca acttctatta tttccagatg ctgaaatcgc
    10141 tggcagatca ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc
    10201 ataaagtggt gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc
    10261 gtggcgatac ctccattcgt cgtcatccgt tcgaaggcgt gctgcataat atggagcgcc
    10321 gctataaaga gacggaatcc agcgcggtac gcgaagaatt agccaagttt atcagtaatc
    10381 gtccgtgcgc cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtgtatgtcg
    10441 agaatacgcc gctgcctgct atctccgaca tgagcattgg tcatgcgatg gaattcttca
    10501 acaatctcaa actcgcaggt cagcgggcga agattgcaga aaaaatcctt aaagagatcg
    10561 gcgatcgtct gaaattcctc gttaacgtcg gcctgaatta cctgacgctt tcccgctcgg
    10621 cagaaacgct ttctggcggt gaagcacagc gtatccgtct ggcgagccag attggtgcgg
    10681 gcctggttgg cgttatgtac gtgctggacg agccgtctat cggcctgcac cagcgtgata
    10741 acgagcgcct gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg
    10801 tggagcacga cgaagacgca attcgcgccg ctgaccatgt gatcgacatt ggcccgggcg
    10861 caggtgttca cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatt atggcggtgc
    10921 cggagtcgtt gaccgggcag tacatgagcg gcaaacgcaa gattgaagtg ccgaagaaac
    10981 gcgttccggc gaatccggaa aaagtgctga agctgacagg cgcacgcggc aacaacctga
    11041 aggacgtgac gctgacgctg ccggtgggtc tgtttacctg catcaccggg gtttcaggtt
    11101 ccggtaaatc gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg
    11161 gggcgaccat cgccgaacca gcaccgtatc gcgatattca ggggctggag catttcgata
    11221 aagtgatcga tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct
    11281 ataccggcgt gtttacgcct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc
    11341 gcggctatac gccgggacgt ttcagcttta acgttcgtgg cggacgctgc gaggcctgtc
    11401 agggcgatgg cgtgatcaaa gtggagatgc acttcctgcc ggatatctac gtgccgtgcg
    11461 accagtgcaa aggtaaacgc tataaccgtg aaacgctgga gattaagtac aaaggcaaaa
    11521 ccatccacga agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgccgtac
    11581 ctgcactggc gcgtaagctg caaacgttga tggacgttgg cctgacgtac attcgactgg
    11641 ggcagtccgc aaccaccctt tcaggcggtg aagcccagcg cgtgaagctg gcgcgtgaac
    11701 tgtcaaaacg cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc
    11761 acttcgccga tattcagcaa ctgctcgacg tactgcataa actgcgcgat cagggcaaca
    11821 ccattgtggt gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc
    11881 tgggaccaga aggcggcagt ggtggcggcg agatcctcgt ctccggtacg ccagaaaccg
    11941 tcgcggagtg cgaagcatca cacacggcac gcttccttaa gccgatgctg taatcgttaa
    12001 ggccgctttc tgagcggcct tttcctttca gagttgcacc agcaatttac gtttttcttc
    12061 cggcagtaaa ttcaccgcct gctgataaga cgcatccacc agataataga tttgcgaatc
    12121 tggcagcgaa ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggcttgg
    12181 acgcacatcg ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga
    12241 aacagctggg cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat
    12301 ctgcgtcgct ttccagtcat tatgcacgct ctgttctgcg cctggttttg ccatgcaata
    12361 ttgtagcaac tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg
    12421 cgatccgccg tggatgcgat gttcccccag ccaaatgcct tgccaggtgc cggtctgaat
    12481 acgcccttta tgcaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga
    12541 aggcatatcg tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg
    12601 gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag
    12661 agaggcggag gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc
    12721 agccagctga ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag
    12781 cgtgagcgtc ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg
    12841 cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagcatcac
    12901 gctctcccgc tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct
    12961 tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg
    13021 acatcgcgtg cggcggtaat atcattatca gaatcgccat aaaaaattcg gatattttta
    13081 tcctgcagcc attgcgattt tgtattttgc cctggtttat cgcccgcaaa gatcaccgga
    13141 ttcatgttgg tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt
    13201 ttcgtcgggc tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca
    13261 atcagctggc gagcgacctc ttttggaatg ctgaattcat cccagccatt gttcattttt
    13321 tcccagaaca caggattttt cagataatct tcgctttctg gcgagaaggt ttttttgccg
    13381 cgccagaagc ccggactgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt
    13441 ggcggacgcc ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aatgggtgcc
    13501 tgttcagcaa gcctggcaac gttagtccca gggttaagcg gtgaaggaga tgaggccagg
    13561 gcaacagcgg aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc
    13621 ttgcgcatat ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg
    13681 caaaaaccat ctgaccataa cgccagctgc cggggaaagg aaggattatc tgcggttttt
    13741 gtgatgaaaa gcaggatgat tatatttaac ccagtaaata cgttcctgtc tttttttgta
    13801 taaaaaatgt ttattactcg atgtaaggat acattaattt tacgtttatt atgatgaatc
    13861 ctcggcaaat gagcgaaatt tgatagaaca tttatctgct gtacgattta atgaacttaa
    13921 tgatacagca ttacaaaaac aatcgaagtt tataaagatg atttctgatt gaccagcccc
    13981 ctaccctaca atggtgatta attttattac tcgcgatgga gatgatgata tgaatatatc
    14041 atatgtgaat agcaacaaaa cgacatcttt acctgttgag ctagatgcac taaataataa
    14101 agatatctct tatgccaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
    14161 agatttttta gacctggaga agaagtatca agttctattg caagtaaagt ttttcacgca
    14221 tttattgatt tagttaacaa aatcaggggt aagaaagact ttatgtatat ttgcacgctt
    14281 tgctgttttg ccgaggaggt taaaggtgat tattctcatt acaggacctt tttatttgat
    14341 attggtaatc aatataaggt taaacttaca caaagcggaa aaaagagttc tctttaacat
    14401 tagaatttaa cgatactata attgaatctc agaaagtcac aggcaataag gcaaagcata
    14461 ttcttgagga tatagaaata ttctatcgta acaaacccga tacttattat taaatttaat
    14521 aatggttaga tgtgcagaaa ttattactcc acccaaaaaa acaggataag gaattccaga
    14581 taattgggat aagcttaaca ataaaagaac taacaacgtt tattatctct cacgtagaac
    14641 gatggcatca aaaaaatcat ctataaagaa agagtgaaag cgacgacagt ttaatttcac
    14701 tgcaggctgg gtagctccag cctgctttcc tgcattacat caccgcagca aacgcctttg
    14761 ccacacgttg tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga
    14821 gatagacacc aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac
    14881 tgaacatgcc gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta
    14941 ataccttcac caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg
    15001 ccagccagct ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa
    15061 aattcggcgg gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc
    15121 cagcggcttc ggcatcttca cacataacag aaagtccgcc gacgcgctcg ccgtaaaggg
    15181 agaaaatttt cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc
    15241 gaatagcgta ggcatcctct tccataccgg caccaaatcc ttgataggca atatcgagga
    15301 atggaataag ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga
    15361 gatcggcacc cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta
    15421 atgttttcag cgtcgccaac aggtcattaa agcgcacgcc gttagtcgct tcgtcatacc
    15481 aggggtaagt actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccagg
    15541 taggatcgct gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca
    15601 ctttcaatgc cccggagccg ccaagggttt gaatggttgc tacgcgctgt tgtttcagta
    15661 ccggatggtc cgcaccaaac agcagcggcg caatggcatg gcgatagcag ttaagccctt
    15721 ccatcggtaa ataaagcgaa gcgccatgag gctgcgcatt caggcgcgct tccgcctccg
    15781 ccacggcttg cagttgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat
    15841 tcactttgtc gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc
    15901 cagcgtaggc gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt
    15961 taaaacacaa taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa
    16021 ttagcaacag agtgagactc atgacaaacg tacatccgcc agaggcacga cctttataag
    16081 agcatgaaag aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc
    16141 acgccgcatc cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg
    16201 cgacccttga agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga
    16261 tacgttctac gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg
    16321 cctgtggacc taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt
    16381 cgcgaccgtt caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat
    16441 agcccatcgc gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac
    16501 caacaggctc tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca
    16561 ctggctgaca gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa
    16621 gaatgatgcc cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg
    16681 acgcggcaat ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt
    16741 gtttctcggt tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca
    16801 ccggctgacg aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg
    16861 gccttacgcc cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt
    16921 ccaggctagc ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat
    16981 gttgcgcaga aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag
    17041 gtttggtgat tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg
    17101 cgtcagcatc ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt
    17161 tcaccaccgc aaccatttta ctggcagggg ccaattcacg aagacgttgc aggttgtgtc
    17221 gcagagcgcg gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataagtgt
    17281 ttgctttaat tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca
    17341 tagttgtcga agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg
    17401 ttacgttgtt taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac
    17461 acctcatcac gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca
    17521 cgcaggtcgg agttgaccgg gcgtttgtcg gcacgttgtt ccagagaacg gttcaactgg
    17581 gacagcgcca ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgagagatt
    17641 tctgcaattt ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag
    17701 tcgatcatga taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc
    17761 acttccgttg gcgtcaggcc ggaggagtca tcgatataga tattgcgttt ttcgagcaaa
    17821 atacccatgg tgccggaaat gcgcgcccag tcttcgtcat cgagctgccc ggtacggatt
    17881 ttagtctggt caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc
    17941 atctccagcg agaagataag taccggttta tcctgcaaca tcgccgcgtt ttcgacgagg
    18001 ttcatcgcaa atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac
    18061 ggctgcaagc cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg
    18121 ccatcgtgtg gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg
    18181 gcgatgttct tcggcccttc gtctttgttc gcacgacttt cggcaatttt aaagacgcgg
    18241 gattcagcca gatccagcag atcttcgctg gtacgcccct gcggatcaaa accagcttcg
    18301 gcaatctcat tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc
    18361 gcataggcac tgatgttagc cgcacttggc gtattttttg acagctctgc cagataagca
    18421 aaaccaccga cgctatcgag ttgcccctgg cgttccagcg attccgcaag agtaatcaga
    18481 tcgatagggc taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg
    18541 cgggtgtaaa aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatct
    18601 agcattaaac cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc
    18661 ccggcaactt gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt
    18721 cctgccatag tgaatggagt taccgagata aagaatgggt cgaaacttta tcatatgaag
    18781 cagaccctga cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac
    18841 ggtggcccgg aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa
    18901 atccaggtcg aaaataaagc catcggcatc aattttatcg acacatatat ccgcagcggc
    18961 ctttacccgc cgccatcgct acccagcgga ttaggcaccg aagcagcagg catcgtgagt
    19021 aaagtcggca gtggtgtaaa gcatattaag gcaggcgatc gtgtagtcta tgcgcagtcg
    19081 gcgttaggcg cttacagctc tgtgcataac attattgcgg ataaagcggc gattctgcct
    19141 gcggcaattt cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat
    19201 ctgctgcgca aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct
    19261 ggcggcgttg gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa acttatcggc
    19321 accgtaggaa ccgcgcaaaa agcgcagagc gcgctaaaag cgggcgcgtg gcaggttatt
    19381 aactatcgtg aagaggatct ggtcgagcgg ttaaaagaga tcaccggcgg taagaaagtg
    19441 cgcgtggtgt acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa
    19501 cgccgcggct taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta
    19561 ggcattctca atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc
    19621 accacgcggg aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt
    19681 gtgattaagg tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg
    19741 catgagattc tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa
    19801 tagggcttcc acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca
    19861 cgatgaatct gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct
    19921 aaggataaaa ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata
    19981 acgctgatat ttcggcactt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat
    20041 cgccagtagt aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat
    20101 aatggccgtc gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat
    20161 cagcatgaca aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg
    20221 aaatctcttg ctggcatcac aagaatcatg ccaaaaataa tctattgatt taacagcaaa
    20281 acgccccgcg acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac
    20341 gcttatccgc caccagtttg agcgcgtgtt ccagcacatt aatgtctgca cccgctttat
    20401 gggcattttc acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaagc
    20461 ccaacatatg ccgggtaata tggccgagat acgtcccctg gctgagttca cgctcaatgt
    20521 acggatacat ggcgcgcact accgccaccg gatcggcatc ggtatccgag gaaccaaaga
    20581 tctcccggtc taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca
    20641 cgccatccat atgttgcagg tgtgctttgg cctcttccag cgacttgata ccaccgttaa
    20701 tcgacattgt cagatgcgga aagtcacgct tcagttgata cacacgcgga taatcgagcg
    20761 gcgggatttc acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga
    20821 tgatgaacat ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt
    20881 catagctgtc ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca
    20941 catcgcgcat cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac
    21001 caaacatgcc attctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat
    21061 atccgcgcgc ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca
    21121 gttgcaacgc taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa
    21181 taatcgcccc tgtggtcacc atttcggtat acagcaacgt attgcgggaa agcagacgca
    21241 agaaatagcg gcaatgtctg tccgtcca
//
LOCUS       DBBGLQ010000075        21065 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.075, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000075 DBBGLQ010000000
VERSION     DBBGLQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.075"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(143..1792)
                     /gene="actP"
                     /locus_tag="ACIYSB_003679"
     CDS             complement(143..1792)
                     /gene="actP"
                     /locus_tag="ACIYSB_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGC5430636.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1789..2103)
                     /gene="yjcH"
                     /locus_tag="ACIYSB_003680"
     CDS             complement(1789..2103)
                     /gene="yjcH"
                     /locus_tag="ACIYSB_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGC5430637.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2303..4261)
                     /gene="acs"
                     /locus_tag="ACIYSB_003681"
     CDS             complement(2303..4261)
                     /gene="acs"
                     /locus_tag="ACIYSB_003681"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGC5430638.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4654..6090
                     /gene="nrfA"
                     /locus_tag="ACIYSB_003682"
     CDS             4654..6090
                     /gene="nrfA"
                     /locus_tag="ACIYSB_003682"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGC5430639.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6135..6701
                     /gene="nrfB"
                     /locus_tag="ACIYSB_003683"
     CDS             6135..6701
                     /gene="nrfB"
                     /locus_tag="ACIYSB_003683"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGC5430640.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6698..7369
                     /gene="nrfC"
                     /locus_tag="ACIYSB_003684"
     CDS             6698..7369
                     /gene="nrfC"
                     /locus_tag="ACIYSB_003684"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGC5430641.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7366..8322
                     /gene="nrfD"
                     /locus_tag="ACIYSB_003685"
     CDS             7366..8322
                     /gene="nrfD"
                     /locus_tag="ACIYSB_003685"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGC5430642.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8342..10060
                     /gene="nrfE"
                     /locus_tag="ACIYSB_003686"
     CDS             8342..10060
                     /gene="nrfE"
                     /locus_tag="ACIYSB_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGC5430643.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGVVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFECLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10053..10436
                     /gene="nrfF"
                     /locus_tag="ACIYSB_003687"
     CDS             10053..10436
                     /gene="nrfF"
                     /locus_tag="ACIYSB_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGC5430644.1"
                     /translation="MNKGLLTLLLLFTCFAHAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10433..11029
                     /gene="nrfG"
                     /locus_tag="ACIYSB_003688"
     CDS             10433..11029
                     /gene="nrfG"
                     /locus_tag="ACIYSB_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGC5430645.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRVNRTQLVESINMAKLLQRRLD"
     gene            11371..12684
                     /gene="gltP"
                     /locus_tag="ACIYSB_003689"
     CDS             11371..12684
                     /gene="gltP"
                     /locus_tag="ACIYSB_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGC5430646.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12989..13678)
                     /gene="yjcO"
                     /locus_tag="ACIYSB_003690"
     CDS             complement(12989..13678)
                     /gene="yjcO"
                     /locus_tag="ACIYSB_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGC5430647.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13772..15919)
                     /gene="fdhF"
                     /locus_tag="ACIYSB_003691"
     CDS             complement(13772..15919)
                     /gene="fdhF"
                     /locus_tag="ACIYSB_003691"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15500..15502),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HGC5430648.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16117..17583)
                     /gene="mdtP"
                     /locus_tag="ACIYSB_003692"
     CDS             complement(16117..17583)
                     /gene="mdtP"
                     /locus_tag="ACIYSB_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGC5430649.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYSMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLATGVAQLYYSMQASYQMLDLLEQTHDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IRPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLHTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAGPVVEKK"
     gene            complement(17580..19630)
                     /gene="mdtO"
                     /locus_tag="ACIYSB_003693"
                     /pseudo
     CDS             complement(17580..19630)
                     /gene="mdtO"
                     /locus_tag="ACIYSB_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(19630..20661)
                     /gene="mdtN"
                     /locus_tag="ACIYSB_003694"
     CDS             complement(19630..20661)
                     /gene="mdtN"
                     /locus_tag="ACIYSB_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGC5430650.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20680..20955)
                     /gene="ytcA"
                     /locus_tag="ACIYSB_003695"
     CDS             complement(20680..20955)
                     /gene="ytcA"
                     /locus_tag="ACIYSB_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGC5430651.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4745 a         5571 c         5765 g         4984 t
ORIGIN      
        1 tattctctgc attatcattt tctacgactg tcaaaaatcg ctcatttttt aatgagttta
       61 tttgtttaat attatgggaa aaggtgatgc atttgggaga ggaagagtat tccccggtca
      121 gacgaccggg gaaggggaga gattaatgcg cgcggccttg ctcaacgcca aagccggtct
      181 gggagcggat aaactgcgcg cggaacagtt cacgctcacg cgcgccttcc gctgagttat
      241 cggttgccga gaagaaccag atgccgagga atgccacggt gatagagaac agcgccgggt
      301 attcatacgg gaagatggct ttttcgtgac caaggatctg tacccaaatc gtcgggccga
      361 ggatcatcag tactactgcg gtaatcagcc ccagccagcc acccatcatc gcgccacgcg
      421 tggtcagttt cgaccagtac atagaaagca gaatgatcgg gaagttacag ctcgccgcga
      481 tggcaaacgc cagccccacc ataaaggcga tgttctgatt ctcaaacagc acgccgagga
      541 taatcgcaat cacgccgagg atcagtacgg tgatttttga tacccgcagc tcttcacgtt
      601 cggtcgcgcc ttttttgaag acgttagcgt acaagtcatg cgaaaccgcg gatgcgcccg
      661 ccagcgtcag acccgcaacc accgcgagga tagtggcgaa agcaaccgct gaaataaaac
      721 cgaggaacag gttgccgccc actgcattcg ccaggtgaac ggccgccatg ttgttaccac
      781 caatcagatg gcccgccgcg tctttatatt ccggattcgc accaaccagc atgatcgcgc
      841 cgaagccgat aataaaggtc agaatataga agtagcccat aaacccggtg gcgtagaaca
      901 cgctcttacg tgcttcgcgg gcatcgctga ctgtaaagaa gcgcatcaga atgtgcggca
      961 agcccgccgt accaaacatc agtcccagac ccagagagag cgcggagatc ggatctttca
     1021 ccagcccgcc cggcttcatg atgtcgacac ctttcgggtg taccgccatc gcttcactga
     1081 acagattgtt gaagctaaag ccgacgtgtt tcatcaccat aaaggccata aagctggcac
     1141 cgaacagcaa cagcacggct ttgataattt gcacccaggt ggtcgccagc atgccgccga
     1201 acaggacgta catcatcatc agcacgccga ccagcaccac cgcaatgtga tagttaaggc
     1261 caaacagcag ctcgatcagt ttacctgcgc ccaccatctg ggcgataagg taaagcgcca
     1321 ccaccaccag agaaccacag gccgaaagaa tacgaatcgg cccttgtttc agacggtaag
     1381 aggccacatc ggcaaaggtg tagcgcccca ggttacgcag acgttcggca atcaggaaca
     1441 aaatgatcgg ccagcccacc aggaagccca gcgagtaaat taagccgtca tagccggagg
     1501 taaacaccag cgcggagatc cccaagaatg aggcggcgga catatagtcc ccggcaatcg
     1561 ccagcccgtt ctggaagcca gtgatattgc cgcctgcggt gtagtagtcg ctacgagaac
     1621 gtacgcgttt tgatgcccag taggtaatgc cgagcgtaaa cacgacgaaa atcaggaaca
     1681 taataatcgc ctgccagttc gttggctggc gctctacggc cccgctaata gcatccgcgg
     1741 cgttagctgc gaaagggagt gtggcggcaa gcgccgtcag aactcttttc atgatgcttg
     1801 tacctcatgc aggacttcat tattaagacg gtcgaattcg ccgttcgccc gccagatgta
     1861 gataccggtg agaacaaagg agatcacaat cactccaaca ccaattggaa taccgcgtgt
     1921 gacgctggtg ttcggattca gcggcgtgcc cagccagccg ggcgcgaagg cgatcagtaa
     1981 aataaagccg atataaactg ccagcataat aatcgacagg atggtggcaa accgttgccg
     2041 tttttcgact aactccctga aatgcgcatt gtcttctatc cgctgataaa tagtgccatt
     2101 catcacagat tctccagagg taatgtaggg attgttttaa ttcccgctcc cttatgggag
     2161 aaggttaacg ctcgggtaac ccttgccgaa tgtaggccgg ataaggcgtt tacgccgcat
     2221 ccggcaatca atgcctgatg cgacgctgtc gcgtcttatc aggcctacaa accgttaccg
     2281 actcgcatcg ggcaattgtg ggttacgatg gcatcgcgat agcctgcttc tcttcaagca
     2341 gcttctcgac tacgccagga tcggcaagcg tcgaggtatc gcccaggttg ctggtatcgc
     2401 ccgccgcaat tttgcgcaga atacggcgca taattttgcc ggagcgggtt ttaggcaggg
     2461 agtcggtcca gtgcagcacg tctggcgtcg ccagcgggcc aatctcttta cgcacccagt
     2521 tgcggacttc tgcgtacagt tctggtgacg gttcctcccc gtgattaagc gtgacgtagg
     2581 cgtagatcgc ctgaccttta atattgtgcg gaatacctac tacggcggct tcggcaatct
     2641 tcggatgcgc caccagcgcc gactcaatct ctgccgtccc cagacggtga ccggagacgt
     2701 tcagcacgtc gtccacacgc ccggttatcc agtaatagcc atcttcatcg cgacgcgcgc
     2761 cgtcgccgct gaaatacata tttttgaagg tggagaagta ggtctgttca aaacgttcgt
     2821 gatcgccaaa cagcgtacgc gcctgacccg gccaggagtc ggtgattacc aggctacctt
     2881 cggtggcccc ctccagcggg ttaccttcgt tatcgaccag cgccggttgc acgccgaaga
     2941 acggacgtgt tgccgaaccg gctttcagct cggtagcgcc aggcagcggg gtgatcatga
     3001 aaccgccggt ttcggtctgc caccaggtat cgaccaccgg acatttctcg ttgccgattt
     3061 ttttccagta ccactcccac gcttccgggt taattggctc gcccacggaa ccgagaatgc
     3121 gcagcgacga acggtcggtg ccttcgatcg ctttatcgcc ttccgccatc agcgcgcgga
     3181 tcgccgtggg tgcggtatag agaatattga cctgatgctt gtccaccacc tgcgccatac
     3241 gggcaggcgt cggccagttg ggtacgcctt caaacatcag cgtggtcgca ccgcaggcca
     3301 gcgggccgta cagcaagtaa ctgtgtccgg tcacccagcc cacatcggcg gtgcaccagt
     3361 agatatcacc cggatgataa tcaaagacat atttaaaggt cagcgccgcg tacaccagat
     3421 aaccgccggt agtatgcagc acaccttttg gcttaccggt agaaccggag gtgtagagaa
     3481 taaacagcgg atcttcggcg ttcatctctt ccgcctggtg ctgatcgctc gcttgctcaa
     3541 ccaggtcgtg ccaccacagg tcgcgccctt cctgccagtc aattttcccg ccagtacgct
     3601 tcagtaccac cacatgctct acgctggtga cgttcgggtt tttcagcgcg tcatcaacgt
     3661 ttttcttcag cggaatactg cgcccggcac gcacaccttc gtcggaagtg atcaccagtc
     3721 gtgagttgga atcaataatg cgcccggcaa cggcttccgg cgagaagccg ccgaaaatca
     3781 ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc aaccgcggct tccggcacca
     3841 tcggcatata aatcgccacc acatcacctt ttttaatgcc cagctcgagc agggtattgg
     3901 cgaagcggca gacgtcgcgg tgcagctctt tatagctgat atgtttgctc tggctggcgt
     3961 cgtcgccttc ccagatgatg gcggtacgat cgccgttttc ttgcagatgg cggtcaaggc
     4021 agtttgccgc cagattcagc gtgccgtcct cgtaccattt aatggacaca ttaccggggg
     4081 caaaggaggt gtttttcacc ttctggtaag gtttgatcca gtcaagaatt tttccctgtt
     4141 cgccccagaa ggtatcaggt acgttaatag attgttgata catcgcctcg tactgctgag
     4201 ggtttatcag gcaacggtct gcgatgttgg caggaatggt gtgtttgtga atttggctca
     4261 tgcttttgtt ctccttgtag gatgttaata atatgtggca taagcgttaa atgtaggggt
     4321 attggcagtt ttgtttagta tttgggcgac agatcacgca aaagtagaat tgtgcaaata
     4381 aacggcaggg taatttttga aggtcagcaa caaaagttga ttaattcttt gaggaacatg
     4441 cagttatgca tgctgttgaa aaagaggaag atactgacta actctaaagt ggtattttac
     4501 atgcacttac aattgattaa agacaacatt cacagtgtgg ttatttgtta cacatagggg
     4561 cgagcaatgt catgacagtg taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca
     4621 ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc
     4681 cgtatcttca gcttattgat tccttttttc tttttcactt ctgttcacgc tgaacaaacg
     4741 gctgctcccg caaaacctgt aactgtggaa gcgaagaatg aaacctttgc cccgcagcat
     4801 cccgatcaat atctctcctg gaaagccacc tcggaacagt cagagcgtgt tgacgccctg
     4861 gcggaagatc cacggctggt gatcctgtgg gcggggtatc ccttctcgcg cgattacaac
     4921 aagccgcgtg gacatgcttt tgctgtgacc gatgtgcgtg aaaccctgcg taccggtgcg
     4981 ccgaaaaacg ctgaagatgg tccgctaccg atggcatgct ggagttgtaa aagcccggat
     5041 gtggcgcgtc tgatccagaa agacggcgaa gatggctact tccacggtaa atgggcgcgc
     5101 ggcggtccgg aaatcgtcaa caacttaggt tgtgccgatt gccataacac cgcctctcca
     5161 gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg
     5221 gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg accagcaatc gatggtttgc
     5281 ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg
     5341 gatgacggca tgaaagtcga aaatatggag cagtattacg acaaaattgc cttctctgac
     5401 tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc agcacccgga atatgaaacc
     5461 tggacagcgg gcattcacgg taaaaacaac gtgacctgta tcgactgcca tatgccaaaa
     5521 gtgcagaacg ccgaaggcaa actctacacc gaccataaaa ttggtaatcc gtttgataac
     5581 ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag ctgccttgca aaaagtggtc
     5641 gcggaacgta agcagtcgat taacgacctg aaaatcaagg ttgaagatca actggttcac
     5701 gctcacttcg aagcgaaagc agcgctggat gcaggcgcga cggaagctga aatgaagcca
     5761 attcaggacg atatccgtca tgcccagtgg cgctgggatc tggcgatcgc ttcccacggc
     5821 attcatatgc acgcaccgga agaaggttta cggatgctcg gtacggcgat ggataaagcg
     5881 gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca aaggcatcac ccatgaaatc
     5941 cagatcccgg atatctcaac caaagagaaa gcccagcagg ctattggcct gaacatggaa
     6001 caaatcaagg ccgagaagca ggacttcatc aaaacggtga tcccgcagtg ggaagaacag
     6061 gcacgtaaaa acggtctgtt aagccaataa ccccgttccg cctcgcaagg ggcggaaaac
     6121 acaatggagt gaatatgagc gtattacgtt cgttattaac tgccggggtg ctggcgtcgg
     6181 gcctgttgtg gagcctgaac ggaattaccg ctacccctgc ggcgcaggca tctgatgatc
     6241 gctacgaagt tacccagcag cgtaacccgg atgccgcctg tctggactgt cataaaccag
     6301 ataccgaagg tatgcatgga aaacatgctt ccgttatcaa cccgaataac aaactgccgg
     6361 tcacctgcac caactgtcac ggccagccat caccgcaaca ccgcgaaggg gtgaaagatg
     6421 tgatgcgctt taacgagccg atgtacaagg ttggagagca gaacagcgtc tgtatgtcct
     6481 gtcatctgcc ggaacagttg caaaaagcgt tctggccgca cgatgtccac gtaaccaaag
     6541 tggcgtgcgc cagctgccat tccctgcatc cacagcaaga tacgatgcag acgttaagtg
     6601 acaaaggacg gattaagatt tgcgtcgatt gccacagcga tcagcgcacc aatccgaact
     6661 ttaacccggc gtctgttccg ttgcttaagg agcagccatg acctggtctc gtcgccagtt
     6721 tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc gcagggcgcg tggtggcgaa
     6781 aacgttgaat atcaatgggg tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg
     6841 ctgtaccgcc tgtatggatg cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg
     6901 cctgacgatt attcgtagcg agccgcaggg cgaatttcct gatgtgaaat atcgcttctt
     6961 ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc
     7021 gtcttttcgc gatgctgcca gtgggattgt cgatgttaat ccggatctct gcgtcggttg
     7081 tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac
     7141 ggcggacaaa tgcgatttct gccgtaagac caatttgcag gccggtaagt tgcccgcgtg
     7201 cgttgaagct tgcccgacca aggcgctgac gtttggcaat ctggacgatc ccaacagtga
     7261 gatttcgcaa ctgctgcgcc agaagcccac ttaccgctac aagctggcgc tgggaaccaa
     7321 accgaagctg taccgcgtac cgtttaaata cggggaggtg agccaatgac gcagacttcc
     7381 gcatttcatt ttgaatcgct ggtgtgggac tggccgattg ccatctacct gtttttgatt
     7441 ggtatttctg ctggtctggt gacgctggcc gtgctgttac gtcgcttcta cccgcaggcg
     7501 ggcggtgcag acagtacgtt gctgcgcacc acgctgattg tcgggccggg cgcggtgatc
     7561 ctcggtctgt tgatcctcgt cttccacctg acaagaccgt ggaccttctg gaagctgatg
     7621 ttccactaca gttttacctc ggtgatgtcg atgggggtga tgctgtttca gctctacatg
     7681 gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa
     7741 cgctggttgc cgaagctggg gatcgtgcaa aaggttctga gcctgctgac gcccgttcat
     7801 cgcggactgg aaacattgat gctggtgctg gcggtgttgt tgggggctta taccggcttt
     7861 ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc cgatcctgcc ggtgctgttc
     7921 ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga tcgccatggc gatacgccaa
     7981 cgcagtaacc cgcattccac ggaagcgcag tttgtacacc gtatggaaat ccctgtggta
     8041 tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt
     8101 aaagttcgtg cgctggtggc ggcattaggt ggcggtttct ggacgtggtg gttctggctt
     8161 ggtgtcgccg ggctggggct gattgtgcca atgctgctca aaccgtgggt caatcgcagt
     8221 tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc tggtcggcgt gttgatgctg
     8281 cgctttttca ttctctacgc cggacagttg acggtggcgt aagccagaaa agaggtggtt
     8341 tctggacgta ttccttcctg aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa
     8401 cgtgttgacc ccgttgacgg ccttcgcggg agtgcggttg cgctggcctg ccatgatgcg
     8461 actcacttgc atcggcattc tggcgcagtt cgcgctcctg ctgctcgcct ttggcgtact
     8521 gacgtattgt tttctcatca gcgatttctc ggtcatttat gtcgcgcaac atagctatag
     8581 cctgctgtcg tgggaactca agctggcagc ggtgtggggc ggccatgaag gttcgctgct
     8641 gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt gcctggcatt atcggcagca
     8701 aaccgatccg ctatttccgc tgacgttagc cgttttatct ctcatgctcg ccgcactgct
     8761 actgtttgtg gtgctgtggt ccgatccctt cgtgcggata tttccaccag caatcgaagg
     8821 ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc tttcatccac cgctgcttta
     8881 ccttggctat ggcggtttga tggtagcggc gagcgtggcg ctggcgagtc tactgcgcgg
     8941 cgagtttgat ggtgcctgcg cccgaatttg ctggcgctgg gcgttacctg gctggagtgc
     9001 attaacggcg gggatcatcc tcggttcctg gtgggcctac tgcgaactcg gctggggcgg
     9061 ctggtggttc tgggatccgg tggaaaacgc ctctttatta ccctggcttt ctgccactgc
     9121 gctgctgcac agtttgtccc tgacacgcca gcgggggatt ttttgccact ggtcgctgtt
     9181 gctggcgata gttactctga tgctgtcgct gctgggcacc ttaattgtcc gttctggcat
     9241 tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc gccgtgccgt tgttcagcct
     9301 gtttgcactg attagccttg cgtctctggc tctgtatggc tggcgagcgc gggacggtgg
     9361 cccggcggtg catttttcgg ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct
     9421 gttttgcgca gtgctactga tcgtgctggt gggaacgctt tatccgatga tttacggcct
     9481 gctgggctgg ggacgcctct ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt
     9541 tggtctgttg atgctggtgg tgattgtgct ggcgacgttt gtctctggca aacgcgtgca
     9601 gcttccggcg ctggtagctc atgcgggcgt gctgttattt gccgctgggg tcgtggtctc
     9661 cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg ggtcagcagg tgacgctggc
     9721 aggatacacc ttccgttttg agtgcctcga tctacaagcc aaaggcaatt acaccagcga
     9781 aaaagcgata gtggcactgt ttgaccatca gcaacgcatt ggtgaactga cgccggagcg
     9841 gcgtttttat gaagcacgcc gtcaacaaat gatggaaccg tcaattcgct ggaacggcat
     9901 ccatgactgg tatgcggtca tgggggagaa aactgggccg gatcgttacg cttttcgttt
     9961 gtatgtacaa agcggtgtgc gctggatctg ggggggagga ttgttgatga ttgcgggcgc
    10021 attgttaagc ggatggcggg ggaagaagcg cgatgaataa agggcttctc acgctactgc
    10081 tgttatttac ctgttttgcg cacgctcagg tcgtagacac ctggcaattc gccaatccgc
    10141 aacaacagca acaggcgtta aatattgcca gccagttacg ttgtccgcag tgccagaatc
    10201 aaaacttact ggaatccaac gcgccggtgg ctgtcagtat gcgccatcag gtttacagca
    10261 tggtggcgga ggggaagaac gaagtcgaaa tcattggctg gatgaccgaa cgctacggag
    10321 attttgttcg ctataaccca ccgttaacgg gtcagacgct ggtgttatgg gcgctgccag
    10381 tggtgttgtt actgctgatg gcactgatcc tctggcgagt gagggcgaag cgatgaaaca
    10441 gcccaaaata ccggtgaaaa tgctgacaac actcacgata ttgatggtat ttctctgtgt
    10501 cggcagttat ctgttaagcc caaaatggca ggcggtacgt gcggagtatc agcgtcagcg
    10561 cgatccgcta catcagtttg ccagccagca aaccccggaa gcgcagcttc aggcattgca
    10621 ggataaaatc cgcgctaatc cacaaaacag cgaacagtgg gcgttactgg gcgagtacta
    10681 tctgtggcaa aacgattaca gcaattcgtt gctggcgtac cgtcaggcgt tgcaactacg
    10741 tggtgagaac gctgaactgt atgcggcgct ggcgacggtg ctttattacc aggccagcca
    10801 gcatatgacc gcccagactc gcgcaatgat cgacaaagcc ctcgcgctgg acagtaatga
    10861 aatcaccgcc ctgatgctgc tggcttccga tgcgtttatg caggcgaact acgcgcaagc
    10921 catcgaacta tggcaaaaag tgatggatct caactcaccg cgagttaacc gaacacagct
    10981 ggttgagtcg attaatatgg cgaaattgtt gcagcggaga ttggattaat catctggtga
    11041 tgaagattat gtcattgagc tgcataaaaa ataatcgaat gaacatatgc caaaaataat
    11101 cactaatcag tattattgca gattaaacaa ataaaaatct ttccataaca aatggttatt
    11161 cattaatcct gccataaact cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt
    11221 aaagggttgc gcaacatacc gcgcaaatga tactgatcat aagcgttaaa aaaatctaca
    11281 aaccaacgca acacaattca tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg
    11341 gaacggcgct ccattgagga agtcattcat atgaaaaata taaaattcag cctggcctgg
    11401 cagattctgt ttgctatggt gctgggcatt ctcctgggaa gctacctgca ctaccatagc
    11461 gacagccgcg actggctggt cgtcaatttg ctctctccgg cgggtgatat cttcatccat
    11521 ctgattaaaa tgattgttgt gccgattgtg atctccacgc tggtggtggg tatcgcgggt
    11581 gttggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa ccattatcta cttcgaggtg
    11641 atcaccaccg tcgccatcat tttggggatc actctggcga acgtcttcca gcccggtgcc
    11701 ggggtggata tgtcgcagtt ggcgaccgtc gatatctcga aatatcagag cactacggaa
    11761 gcggtacaaa gcagttccca cggcattatg ggcacgattt tgtcgctggt gccgacgaac
    11821 attgtggcgt cgatggcgaa aggcgaaatg ctgccgatca tctttttctc ggtgctgttt
    11881 ggtctggggc tttcttccct gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc
    11941 tccatctctg aaaccatgtt taaagtgact cacatggtga tgcgttatgc accggtgggt
    12001 gtgtttgcgc tgattgcggt gacggtggct aactttggtt tctcgtctct gtggccactg
    12061 gcgaaactgg tgctgctggt gcatttcgcc attctgttct tcgcgctggt agtgctggga
    12121 attgtggcgc gcctgtgcgg gttaagcgtc tggatcctga ttcgtattct gaaagatgag
    12181 ctgattctgg cgtactccac tgccagctct gaaagcgtgc tgccgcgaat tattgagaag
    12241 atggaagcct acggagcacc ggcgtcgatc accagtttcg tggtgccgac cggttactct
    12301 tttaaccttg atggttcgac gctgtatcaa agtattgccg ctatcttcat cgcgcagttg
    12361 tatggcattg acctgtccat ctggcaggaa atcattctgg tgctgacgct gatggtgacc
    12421 tcgaaaggga ttgctggcgt gcctggcgtg tcgtttgtgg tgttgctggc aacgctgggt
    12481 agcgtaggta ttccgctgga aggtctggcg tttattgctg gtgttgaccg tatcctcgac
    12541 atggcgcgta ctgcgctgaa cgtggtgggt aatgcgctgg cggtgctggt gattgccaag
    12601 tgggaacaca aatttgaccg taagaaagcg ctggcttatg agcgtgaagt gctgggcaaa
    12661 tttgataaaa ctgcggatca ataattgaag attgccgggg atatccaccc ggcaatgtgt
    12721 gaatgcctga tgcgacgctt gccgcgtctt atcaggccta cgccagacag cgcaatagcc
    12781 tgatttagcg tgattttgta ggtcggataa ggcgtttatg ccgcatccga catcaacgcc
    12841 tgatgcgacg cttaacgcgt cttatcaggc ctacgccaga cagcgcaata gcctgattta
    12901 gcgtgatttt gtaggtcgga taaggcgttt acgccgcatc cgacatcaat gcctgatgcg
    12961 acgcttgccg cgtcttatca ggcctatctt aaccgttggt taatttttca aactcttcac
    13021 acccggtatc aaacccttcc atacagctca ggttcaacca gtgcagcgcc ttttgcttgt
    13081 tcttctcgat aaaaccttct tcaccgttta agaacatcat tcccgcccag tactcggaat
    13141 aaccggttcg ggaaattgca gagctgcgtt tgaaatacca ggttgccttg tcatcgtcgg
    13201 ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag catttgggca tcgacggcag
    13261 agtcgttctc cagatcttcc gaggcgtttt ctaacagcga aattgccttt ggataatccg
    13321 gtttacccgc ttgagtattt accagaatat gcgccagcgt tacttcacct tctttactgc
    13381 ccgcttgcgc cgctttctct gcaagaactt ttgcttgtgg atagtccaga ctgaccggat
    13441 tggtgatttt gatctgcgcc agcagcgcgc aggcatcagc atcaccgctg tcggcggctt
    13501 tctgtgccca atattcggct ttgctcaaat cgccggagct aaaccagctg tcggcaagaa
    13561 aatattgtgc gcgtcggtcc ccggcctctg ctgcctttaa atactggctg ccaggctcgg
    13621 agtcgttggc gtgggcaaag aatgtcaaaa acaacatcaa tgcgataatt tttttcattt
    13681 tgataatacg attgggtagg gagtaaccag tataaagagt tcgaatggat aaaaaaacag
    13741 cctccgaaag gaggctgtag aaaggacggt attacgccag tgccgcttcg cgcaggcgag
    13801 ttttcaactt gttgtactcg tcaatcacgt actgctcggc ggcgcgctga tcggcgatcg
    13861 gctcgacgcg aacggcgcag tatttgtact ccggcgtttt cgtaatcggg cttaagtttt
    13921 cggtaaccag ctcgttacag gcaccaatcc accactggta ggtcatgtaa atcgcccctt
    13981 tgttcggacg atcgctgacc tgcgcacggg tgataatttt gcctttacgc gagtgcaccc
    14041 aaaccaatgc ctcatcttca atacccagac gtttggcgtc ttcggtattg atttgtgcgt
    14101 agccaggttc atcagccagc gccgccagtg ccgcacagtt accggtcatc gaacggcaag
    14161 agtagtgacc aacttcacgc accgttgaca gtaccatcgg gtactcgtcg gtgagtttgt
    14221 cgattggcgc tacccagtcg caggtgaaga actgcgccag accgttcggg gtatcaaact
    14281 tctctttaaa cagataagaa gtcccctgat cggcatctga agtatcgcgg caaggccact
    14341 gaatgaagcc cagttcgccc attttctcgt aagtcgcacc gtagaaatcc gggcacagat
    14401 gacgcaactc atcccagatc tcctgggtgt tgttgtagtg catcggataa cccatacggg
    14461 tggcgatttc actgatgatt tgccagtccg ttttcagatc ccatttcggt tcaaccgcct
    14521 tgaagaaacg ctggaagcca cggtcagccg cagtaaacac gccttcatgc tcgccccacg
    14581 acgttgacgg taaaataaca tccgccgccg acgcggtttt ggtcataaag atgtcctgaa
    14641 cgataaccag ttccagatct tcaaaggctt tacgtactgc cgacagctcc gcgtcagttt
    14701 gtagcggatc ttcgcccata atgtacgcgg cacgcacttc gccatgcgct gcgcggtgcg
    14761 gcagctcgct gatgcgatag ccggtatgcg ctggcaggct ttccacgccc caggctttgg
    14821 cgaatttctc gcggttagcc ggatctttca cgtactggta tcccggatac gtatccggca
    14881 gcgcgcccat atcgcaggca ccctgaacgt tgttctgacc acgaaccggg ttaacacccg
    14941 catgcggctt accgaggtta ccggtcagca tcgcgaggct ggtcagagaa cgcacggttt
    15001 ccacgccctg gtagaactgg gttacaccca tgccccacag gatggcggcg cttttcgcct
    15061 gggcatacat ccgtgccgcc tgacgaatct cactggcgct gacgccggtg atatcttcaa
    15121 ccgactccgg cgtgtagcct tcaacgattt tacgatactc ttcaaagcct tctgtacgtg
    15181 aagcgacgaa cgctttgtcg tacagatttt cttcaataat gacatggccc atcgcattca
    15241 acagcgcgat gttcgagccg tttttcagtg caatgtgcat gtcagcaatg cgcgcggttt
    15301 caattttgcg cggatcgcag acgataattt tcgccccgtt acgtttagcg ttaattacgt
    15361 gattcgccac gattgggtgg gaatccgccg ggttgtaccc gaaaacgaac actaaatcgg
    15421 tattatcaat ttcgttaata gcattgctca ttgcgccatt accgaccgat tggtgcagac
    15481 ctgcaaccga tgggccgtgt cagacacgag cgcagcagtc aacgttattg gtaccaataa
    15541 cggcgcgcgc aaatttttgc attacatagt tggtttcgtt acccgtacca cgcgaggagc
    15601 cggtcgtctg gatggcatcc ggaccgtact tctctttgat ggcgctcagg cgctcggcaa
    15661 cgtaattcag tgcctcatcc caggaaacag gttcgagttt gccgccacgc tggcgacgga
    15721 tcatgggggt tttcaggcgc ggggtcagga tctgggtatc gttaatgaag tcccagccat
    15781 aataaccctt cagacacagg gtaccctggt tggttttccc ctgcgctgcc tccgcccgga
    15841 cgattttacc gttatcgacg accaggttga ttttgcaacc tgatgcgcaa taggggcaaa
    15901 ccgtgacgac ttttttcatc ggtctcgctc cagttaatca aatcacgcat acgcgctctc
    15961 gactacagta tgcatctttt atgccacatt ttatgtgggg tcattccctg atattacggg
    16021 cactatttat tcaaaactct gacgaaaaac aggctgtcgt cagttttgac gtgacgaaac
    16081 gaaataccgc gtgacagcca tcacgcggca gacattttat tttttctcga cgacgggacc
    16141 tgcctgatac ccgccgccca gcgatttcat caactgaatg ctttggatca cccggcggct
    16201 gtccagcatc agtaatgaca tctcttcggc aagcactggc aaccgggctt cggtggcctg
    16261 taagcggctg gttaagccgc gctgataggc ggcctcggca gcgcgctggg taaagcgcgt
    16321 ggcttccacg cgttcagcct gcatttctcg ctcgtcgttg agcgtttgca gacgcgtgcc
    16381 gttgacggca acgtcacgca ccgcgttcag tactgactgg ttgtaacgtt caatcatcat
    16441 gttgctggcg gcgcgcgtgc cttcgagatt ggcattcaac cgtccaccgt caaacagcgg
    16501 caatttcaga cccgggatga agttgaactg gcgactggtt tttttgaata aggtatgcag
    16561 atggatggag tccagaccga aaaacgcttt gatatcaaag ctcggataga acaacgcccg
    16621 cgcggaatcc acctgatcta atgacgcctg aacataccag cgcatggctt gcagatccgg
    16681 gcgtctggcg agcaactcat aagagagtgt cgccggaatg ccggtctgga cttgcggtaa
    16741 tgccaccggt ctgatctccg gcatatcgct ggctcccgcg ccaatcaatg cacgcagaga
    16801 ttctcgcgtt tcggtgattt gccctttgac ggcaacaatt tgtttatcga ccgccagaat
    16861 ctgtgcccgc gcgccgtgga aaggcacttg cgcttccaga ccgtgcgcca ctttactctg
    16921 gtgcgctttc accgcgtaat caatcacatc gtgagtttgt tctaacagat cgagcatctg
    16981 atagctggcc tgcatactgt aataaagctg cgctacgccc gtggccagcg atagctctac
    17041 tgctgcggtt tctgccagcg cggcattatg cgcgccaatg gcggcggcaa ccgctgagcg
    17101 atgcacaccc cacaaatcaa gatccagtcc ggcaaacaaa cctactgtgg cttccgtata
    17161 gtacggcccg tccataccca gtgctggcgc atccattgaa taagggctta aaaagccgtt
    17221 cgccgagaca cgttggcggt tgagcatccc taacgctgcg acctgtaatt gtgaaccggc
    17281 atctaacaaa tcggcctggg actgcgcttt ttcttcccgc agtttcgctt cggcgagggt
    17341 gtgtgaacca cttagcgtcc gttggatcag cgcatccagc tgcgggtcat tgagttgttt
    17401 ccaccactgc gcctgcggcc agccggagct ggcaagatga atatcgtcgg ccagtttgat
    17461 ttgttccggt ttgagctgtt gatgaggtgc agaatcctta cgtaccaggg cacagccgga
    17521 aatcagcgtc gtgctgccga gaatgctgca caacagcaga cgtgaaagtt gacgattgat
    17581 cattgcgtgg ctccttgtgc ctgttccgtg gcgggcgtta atgccggggc tgtccagtct
    17641 ggcaagcggg cgaaaagctg gcagacgtgt tgctcctgtt ttaataaggt gggcaggatg
    17701 gcctgagagg cgggtgtctc ttcaagcgtt atttgaggag aacggctgag tgcggttgcc
    17761 agaccggcag cgtatttctc cagggcgtcg gcaaactgac ctgctctgtc cggctgtagt
    17821 gcgttatcca gcgcctgcgc cgagttccag gtggcatccc aggcgtgaag aagatcgcgg
    17881 ccctgacgaa taaccgtttg cgaacgttca atcagtaatg cgcgttcttc gctgtccagt
    17941 tgacgctcca gcgccacgcg ttggcacatc tcttcacagg cattaaacgc cgcatgcaga
    18001 ccgatacgaa tttgcagata agtgcgcaga gccgtgactt cctgctggcg tgggatccgc
    18061 attactttac ttaacatacc cagcgtgcca gccagttttt gcggcagtgt gcgcgcttca
    18121 ctttcaggcc agacaaaggt gtaaatcacc gcggacacca cggtaccaat gatgataccc
    18181 agggcgcgat cgcgaatttc caccaggtcg tacactgggc caaaaacgtt ttcgagcgtg
    18241 gcgagcgcga aggtgaccac catctgtgtg ccgatataag aagagcgttc agagctggtg
    18301 gcaatccatg cgcccaacag gaaaatcggt gccagcacaa acagcaattc gacaatattg
    18361 tccagccagg gcatgaccag tagcgtgaat aacagcgcca gaatcgcgcc gcaaaaggcc
    18421 ccgccaaaac gcagcaccat cttctggtac gacgaaccga catttgggtt agcgacgatc
    18481 acgcatgtca gcatacaggt gtgaatgcct tcccagtcca cgcagctgta aaaggtgtaa
    18541 cagatcaaac aggcgagcag cgtttttacc gcgtagcgca tatagtctgg attggtaaaa
    18601 gcatcggcgg ccattgatgg cggtttggct gcgggcgttg gcggcgtatt cgggtccatc
    18661 tgacccagtt gtaacaacgt ctggcagata ttctccaggt tacattcccg tgccgccatc
    18721 gcttcacttt cactgatccg ccagtcgctt tgccagcact gaccttcagc aacggcatgc
    18781 tgcagcttgt tgatttctga agctaatttt tgtcggaatt caataattgc ctgagaatca
    18841 gcaaaagagg tgggatcgta gcgattcagc gtcgagtaaa tgtaggttac cgttgccacg
    18901 cagctttgcc accatgcgtt ttgagttcgc cagttggcat cgtccgcgag gcaaaagaca
    18961 ttgagttttt gtagcgccag cgcctctctt tcaatccgcg tttcgggtag cggtgcgagg
    19021 ctgtctgtca ggtgactaat ggcatcatca agccgatcat taagcgcctg atgcatttgc
    19081 gaaatggcac gactgggaaa ccacagcacg ccgattaacg tcatcagcaa ggttggatag
    19141 aggccaacaa cgatacacta cagcgttaag cgcacgacca cttccggata gtcgagcatg
    19201 gcggggaagg tttgcccgta aatagcgaca atggcgacgg cgaaaaagac cagccccaag
    19261 cgatgggtgc gcatcaaaaa catgcagccc atcaggatcg gtccggcgat gatcaatcgg
    19321 atcaacggtt cgccgtatga ccatttgtag atcaaaaaca ggctgccgat ctccagcacc
    19381 gtggcaacca caaacaagat cgcgacaaat ttggtgtaaa acgcgttcac tgaataccgt
    19441 aaaacagcac tgccagcgat aacgccacaa aagggatctc aaaggtcatc gagatcagaa
    19501 tcaccagcag gcagcctacc cagagttgca cggtctgtgg cacgcgacct ggtcgccgct
    19561 cgcttaactc ttcatgaaag aacgccagca gcctgaccac cggtaatggc agggagttga
    19621 gcgcgctcat tattgcggct caagattagc gactgccgaa gcgccgatgc ggaacatttc
    19681 cgggtcaggt ttatcgacca tgattttgac cggaaaacgc tgggcaacgc ggacccagtt
    19741 aatagaacga gacactttcg gcaggccgcc cagcaccagg ccgccgtcat ccggtagcac
    19801 gccgtagcca atcgaatcca ctttaccctc gaaggttttg ccgctgtcac tcatcaggcg
    19861 aatcgttgcg ggtgtacctg agcgaatatt tttcagatcg gtttcgcgga agttggcgat
    19921 cacataccag tgacgagtgt cgattagggt aaaaataggg cgcatggcag aagcaaattg
    19981 cccgacggag gttttgaggg aaatgacccg gccatcaaac ggcgcgcgaa cggtcgccat
    20041 ttccagatgc agtttggtca gggcaatatc cgcttcgacc gccgcacgct gggcaactaa
    20101 tgcatccacg ccgctgacgg cgctggcggc tgactgcgcc tgtaacaata cggcattaag
    20161 atccgcttct gcggcgcgct gcgccgttct tgcacggtca acatcttccg ctgagacaaa
    20221 accttctttc agtaatggct cggtgcggcg taatgtatct gtggcctgtt tcgcggcggc
    20281 acgggctttt tctaccgtgg cattaaccga gtcggcacca aactgttgcg cgtcaacgct
    20341 acgctgggtg agcataattt gcttatccag cgccgcgagg gaggcttcag ctttcgccag
    20401 attggcttcg tacgggcgcg ggtcgatgcg gaacagcaaa tcgccctgtt tgactgcctg
    20461 gttgtcggtg accgccagtt ctacaatgcg gccgctgact tccggtacca catcaatggt
    20521 atctgctgac gcgtaagcgt cattagttga tggcgcactg tctacgcgcc agataacgaa
    20581 aacaagggca accagcgcca acgcaaccac taacagagca gggaatttac tgcgaggagc
    20641 ttttttcggc gtactttcca tgagcgcgtc tcaaatagat tagaaaaatg ccagccagaa
    20701 cagcatggcg tagagagcaa aaagggcggt ataaataatt ccgacaaatg ccagattgat
    20761 atttgcccgc tgaataactc gcctcgtgat gagcgtcaaa ataaggctgg caatggcgca
    20821 gaaaaaccag ctgggataat aagcgccgat caccgggatt gccggagata atgagcatcc
    20881 cgaaaccatg aagacgggaa ttatccaggc tgtttttttg agttgcatcg ccatgcgaga
    20941 gagaactgtt ggcatattaa atacatcctg tctgctgcga acaatatctg tagtgaatat
    21001 caatatccat tgaaggcgtc agtatggcta ttttcttgat gaataaaaat agctttattc
    21061 agcca
//
LOCUS       DBBGLQ010000076        20551 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.076, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000076 DBBGLQ010000000
VERSION     DBBGLQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.076"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..1231)
                     /locus_tag="ACIYSB_003696"
     CDS             complement(<1..1231)
                     /locus_tag="ACIYSB_003696"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGC5430652.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANA"
     gene            complement(1243..1530)
                     /gene="eutN"
                     /locus_tag="ACIYSB_003697"
     CDS             complement(1243..1530)
                     /gene="eutN"
                     /locus_tag="ACIYSB_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGC5430653.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(1637..1930)
                     /gene="eutM"
                     /locus_tag="ACIYSB_003698"
     CDS             complement(1637..1930)
                     /gene="eutM"
                     /locus_tag="ACIYSB_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGC5430654.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(1969..2985)
                     /gene="pta"
                     /locus_tag="ACIYSB_003699"
     CDS             complement(1969..2985)
                     /gene="pta"
                     /locus_tag="ACIYSB_003699"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGC5430655.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVEAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(2982..3785)
                     /gene="eutT"
                     /locus_tag="ACIYSB_003700"
     CDS             complement(2982..3785)
                     /gene="eutT"
                     /locus_tag="ACIYSB_003700"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGC5430656.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(3782..4483)
                     /gene="eutQ"
                     /locus_tag="ACIYSB_003701"
     CDS             complement(3782..4483)
                     /gene="eutQ"
                     /locus_tag="ACIYSB_003701"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGC5430657.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(4458..4937)
                     /gene="eutP"
                     /locus_tag="ACIYSB_003702"
     CDS             complement(4458..4937)
                     /gene="eutP"
                     /locus_tag="ACIYSB_003702"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGC5430658.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFNHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(4950..5285)
                     /gene="eutS"
                     /locus_tag="ACIYSB_003703"
     CDS             complement(4950..5285)
                     /gene="eutS"
                     /locus_tag="ACIYSB_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGC5430659.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(5578..7857)
                     /gene="maeB"
                     /locus_tag="ACIYSB_003704"
     CDS             complement(5578..7857)
                     /gene="maeB"
                     /locus_tag="ACIYSB_003704"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGC5430660.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSSLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            8146..9096
                     /gene="tal"
                     /locus_tag="ACIYSB_003705"
     CDS             8146..9096
                     /gene="tal"
                     /locus_tag="ACIYSB_003705"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGC5430661.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            9116..11119
                     /gene="tkt"
                     /locus_tag="ACIYSB_003706"
     CDS             9116..11119
                     /gene="tkt"
                     /locus_tag="ACIYSB_003706"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGC5430662.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(11214..12257)
                     /gene="ypfG"
                     /locus_tag="ACIYSB_003707"
     CDS             complement(11214..12257)
                     /gene="ypfG"
                     /locus_tag="ACIYSB_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGC5430663.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGDQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(12383..12958)
                     /gene="nudK"
                     /locus_tag="ACIYSB_003708"
     CDS             complement(12383..12958)
                     /gene="nudK"
                     /locus_tag="ACIYSB_003708"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGC5430664.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNTKKKTVVLIRQFRVATWVNGNESGQLIESCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(13026..15005)
                     /gene="aegA"
                     /locus_tag="ACIYSB_003709"
     CDS             complement(13026..15005)
                     /gene="aegA"
                     /locus_tag="ACIYSB_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGC5430665.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNYIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTTGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            15211..16911
                     /gene="narQ"
                     /locus_tag="ACIYSB_003710"
     CDS             15211..16911
                     /gene="narQ"
                     /locus_tag="ACIYSB_003710"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGC5430666.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            17075..20188
                     /gene="acrD"
                     /locus_tag="ACIYSB_003711"
     CDS             17075..20188
                     /gene="acrD"
                     /locus_tag="ACIYSB_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGC5430667.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(20287..20346)
                     /gene="ypfM"
                     /locus_tag="ACIYSB_003712"
     CDS             complement(20287..20346)
                     /gene="ypfM"
                     /locus_tag="ACIYSB_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGC5430668.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4684 a         5602 c         5356 g         4909 t
ORIGIN      
        1 tagcattcgc catctggttc atgttttcga tgttgcgcga gtgcattgcc gccgtgtggt
       61 ggcaaccgcc ttccagtttc accgctagcg caatggcatc cgccacgttg gcgacgcgca
      121 cgacgggcaa caccggcatc atcagttcag tcacggcaaa cggatgttct gcggtggttt
      181 ccacaaacag caggcgcgtt tcttgcggaa ctttaaggcc gattgccgcc gcgattttgc
      241 ctgcgtcgcg accaacccag tcacggctga cggtgccttt tccgcgctcg tcgatatttt
      301 tcagcaacac cggttgcagc tgctgcgcct gttctgcggt cagtttcacc gcgtgctggc
      361 cttccatcag acgcatcagt tcatcggcta cgctatcaac aacaatcagt accttttcgt
      421 cggcacaaat gatgttgtta tcgaaagaag cgcctttgac gatggactga gcggcacggg
      481 cgaggtcggc ggtttcatcc accactaccg gcgggttgcc agcgcctgcg gcaatcagac
      541 gtttattggt gtgtttacgc gccgcttcta ctaccgcttc gccgccggtt accaccagca
      601 ggccgatacc cggaaacttg aacaagcgtt gcgcggtttc gatatccgga tttgccacag
      661 taaccagtaa gttttccggc ccacctgcgg caacaatcgc ctggttgagc agcgtaatcg
      721 cccgctggga gacttttttc gccgccggat gcggggcaaa aatgacgctg ttgcccgcgg
      781 caatcaggct gatggcgttg ttaattacgg ttgccgccgg gttagtggaa ggcgtcaccg
      841 aagccaccac gccccagggt gcgttttcaa ttagggtcag gccgttgtcg ccagtcagca
      901 cttgcggaga gaggcactca acgcctggtg tgccgcgcgc ctgagcgacg ttttttgcaa
      961 atttatcttc aacgcgcccc atgccggttt cactgacggc aagttccgct aaatctctgg
     1021 cgtgtttttc gcctgcttca cgaatggcag caatggctaa ctggcgcatt gccacgcttt
     1081 ttaacccttg ctgggcgact ttggctgccg caacggcgtc atccagggac gcgaaaacgc
     1141 ccatctcatg aacggcggcg gacggcgtgt cactgctttg cattttcagc agtaccgctt
     1201 tcaccacctg ttcaatatcc tgttgattca tgatgttctg ccttatttgt ggaaaattac
     1261 ctgaccgcca gacaccacct catcgacaat gccaatcacg cacagatcga ccggtgacgt
     1321 ttcgctttta tgcgcctggc gggcggaact gccactcacc agtaacaccc actccccggt
     1381 tcccgcgcca atattatcga tggcgacggc gcattgcccg tcaggattac cttgtggatc
     1441 aatcatttcc accatcagca atttgtcatg cgccagtccg tgatggcgta cggtacaaac
     1501 aatttgtcca gtgacgactg ccagtttcat acccgcctcc gtggcgtatt tcaggtaaaa
     1561 gctcccccta ccctccgcag aaggtaaaat gaaaaaggag agagcgtgac gcccgaatcg
     1621 acgtcacaca gggtgattac aggttgctgc tatcgccttt caggccgatc gggaagactt
     1681 cttccaggtc gccgtgcgga cgcgggataa cgtgtacaga taccagctcg ccgatacgct
     1741 gtgccgctgc ggcacctgca tcggtcgcgg ctttacaggc tgcaacatca ccacgaacca
     1801 tggcagtaca caggccgccg ccaatctgct taacaccaac cagcttgacg cgcgcagctt
     1861 tcaccatcgc gtcagaagcc tcaatcagtg caaccaggcc ccgggtttcg atcattccta
     1921 atgcttccat tgtgctttcc tctttatcag ggtccagaac gggaccgttc attcaaccag
     1981 tgtttgtaaa ctgctttcgc ggttcacttc tgtctgacgc ggcacagctg ccaccagcgc
     2041 cagctcgata atttcctgca cgctacaacc acgagagaga tcgtgcatcg gcgcggcaag
     2101 tccttgtatc agtggcccga cggcacgata tccgccgagt cgttgtgcga ttttgtaacc
     2161 aatatttccg gcttccagcg acggaaaaac catcacattg gccttgccct gtagcgggct
     2221 ggcaggcgct ttttgcgcct ccacttccgg cacgaaggcg gcgtcaaact gtaactcgcc
     2281 atccaccacc agctttggtg cgcgctcacg gacgatttct gtcgcctgct ggacgttagc
     2341 aacacagggg tgacgggcgc taccattgct ggaaaacgac agcatcgcca cgcgcggctc
     2401 ttctccggtg atggcgcgcc aggtttcggc actggcaagc gcgatatccg ccagctgcgc
     2461 cgccgtcggc tgtggcacca cgctgcaatc ggcaaagccc aacgccgggc cgctgtactg
     2521 tggcaacatc aggaaaatgg aagagagcgt tttacagcct ggctgcaagc cgataatgcg
     2581 taatccggca cgcagcacat ttgccgtgga agagaggttg cccgcgatac agacatccgc
     2641 tttaccggcg ctgaccattg ctgcggcgaa catcagaggg tcggtaagtt tttccagcgc
     2701 atccggcggc gttttttcgc ccgcgcgggc cagccagcga tgagcaaatt cttcccgcat
     2761 tgcgaggttg ccatgcggat caatcacctg tagcccgtcc atcgccacgc cgtgactgag
     2821 cgcaaactga cgaagttcaa acggattggc gaccagaatg ggcgttgcca gaccttgttg
     2881 atgtaaatat tgcgcggctt tcagcacacg ttggtctaac gcatccggaa aaaccactct
     2941 ggcgggcgct cgcaacgcca gttcacgaca acgttcaata atcattgcgt ttcccccagt
     3001 cgttgttgga tctgcttcac cgttagcggc tgcttcgtta cgctcaggat catcatcacg
     3061 tagaccgtgc tcgaaaggcg gttcagcgcc tgcaaaatgt ccgggcgtaa tacttcaaag
     3121 ctgcgggtga taaagacctg cgccgctacg gtttcggttt cccgcacttt ggtgcgtagc
     3181 agattgagca acgcagcatc gcgcccgtgg ctggcttccg gcaccagatg atcgtggtcg
     3241 agatagcgca gcggctgatg agaaagccga tgcagatctt cgtcagaaag cccgacaatc
     3301 gcctggcagc ccagcggttc ccccagcgca tcggcgcgca taatgttgcc aagacgcgaa
     3361 cggatatccg ccagccacgg ctgccacggt tccgccagtt caatttgcag ccacactgcc
     3421 agcgcaatgg tgctgtcgag aaccgcgcga aaacccagac gcggatcgct tttggcgacc
     3481 attttttccg ccgacaggtg agtcagcgta tccggctttt ttgccaccgg ctggcgacac
     3541 agttcgcagc acgcctgcgg atgttcatcg ctactggtca gcccatgaac gggctgcggc
     3601 tgctgctgtt catcgtcaac aaacaggcgg ccctgctcgt caataaactt gatgcgcaga
     3661 tggcggcttt ccagcaactc ccgggcagag ggcgtcaggc gactgtccgc agggagatgg
     3721 atctctgcgc cttcgctgag cgtatggttc gctcttagcc atgcttcggt gatgaaatct
     3781 ttcataggga ttgccagtta gccggccagg cgacatacag gaatttcacg ctggatgtcg
     3841 taccaaattc gatgctggag cctttcggga taaacatcac gtcgcctgct ttggcgatca
     3901 tggtttggcc ttcgtggcga acatgcagct ccccttccag caccatgtca atttcgtcgt
     3961 agttcagggt ccacgggaag aatgcgttct cccactgcat aaaaccggcg gccatgctgc
     4021 tgccgtcgtc tccggtcaca agatcagtca agcccacgca atgcggctct gcgccgtcga
     4081 aacgaccaaa cttgacgcta ctgccatcaa tcactttgat gccgcctttg ccggtcaccg
     4141 atttgaagct cggctgcatt gcgccctgct ccagcgactg tttttccttc atcacttttt
     4201 ccatcagctg tgcgaccagg ctttcggtaa actggccttc cggcagctgg gcaatgatgg
     4261 tttcgcggat gcgctggctt tcggttttgt cggctggcac gctggcgggc acggacgccg
     4321 ttaccgggat cgattcatca cattcggtga tggtaaaacc cagtagatcc gccacttcac
     4381 gggcttccgg ggtaataatg ctggcgcgca gaaccacgga cattgcctgt tcgccgcgcg
     4441 cgtgtgcttc acgaatatca ttcgctgtga tgagtttttt cacctgcttc ctcctgtttg
     4501 gtgagagatg ccagataatc caccagttgc tgcacgcttt gcgggtcgtg gctgtttagc
     4561 tcgaaaatcg gctcttcgaa acccgtctca agcaataact ttcgcgttgc ggcgacgtca
     4621 gcatctggca tgtccgtttt actgatgacg gcgatttgcc gtttgctgac gccaatatcc
     4681 agcaacccgg caggcaaccg actttccggg tcatttgcgc catggacata gatcagcatg
     4741 tctacatcct gtagcgtggt aattaaggcg tgataccagc ggggatggtt aaaatattca
     4801 cccggcgtat caatatcgcc cttatcatta aattccacgg cctgtgtttt tctggcgagg
     4861 gtataattcc cctgcaacgc attaaataac gttgtttttc ctgccccgac gctacccaca
     4921 aacgcaattc gtttcatgaa tgcctcgtat taacttttgg tcatttcaca aagcgtataa
     4981 tttaataagc gacctaaacc gctgacggtt tgtgataacg cttcttctac tgcgcccacc
     5041 gagccataaa tcaccaatgc accgctgaat cggtcgagaa aaccgatatg cacatcagcg
     5101 gctttcagcg ctaaatcacc ggcgatcatc gccgtttcgc cgggagttag cgtcataatg
     5161 ccaattgcgc ccgcatccgg aacgccgatt ttcttcgcca gttcctcacc gggatgcgcg
     5221 atgagatgcg ccagtgtgac ctgtttgccg ggcacaaatt cctgaatgat gcgttcttta
     5281 tccatgagtc gccgcctgtg aaaaactttc gtgtatcgtg acaatttttc gtcagagtaa
     5341 ataacaaaat tcggcaagcg cgtttaaaag gtgaggtaga tcactaagaa tggagagata
     5401 ttcgctgtgg tgcataaact ttcatatgca acgcatgaat ataaataaaa taatatcgag
     5461 atattatata gactgctttt ttttattatt cgcaaactca gtaataagct ttactaatat
     5521 attgcagcta aactgcttac cctgaatatt cagggtaagc gtgagagtta aaaaaaatta
     5581 cagcggttgg gtttgcgctt ctaccacggc cagcgccacc atgttgacga tacgacgcac
     5641 cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccaca cccatcagca ccgggccgac
     5701 agtcacacct tccgagctgg aaacacgcag taagttgtaa ctaatgcggg cagcttccat
     5761 gttcggcatc accagaatat tggcggaacc tttcaaagag ctgtccggca tacggtcgtt
     5821 gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca ccatcaatca tcagttctgg
     5881 tgcacgttcc ctgaccagtt ccagcgcctg acgcattttg ctcgacgacg ggcagtcaga
     5941 agaaccaaag ttggagtgcg acaacaaagc aacgcgcggc tcaataccaa aacgacggac
     6001 agtttctgcc gccatcaagg tgatctccgc cagctcttct gcatccggtt catcattaac
     6061 atatgtatcg gcaataaagg tgttaccact cggcagcagc agcgcgttca tggcacctgc
     6121 ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc acgctaaaat gttcatgata
     6181 atcacccacc gtaccgcaaa tcattgcatc ggcttcccca cgctgaacca tgatcgcgcc
     6241 gatcactgtc gggttactga tcagcgcccg ctgcgcctgt tcctgagtga cgccgcgacg
     6301 cttcatgatc tggaagtatt cggtccagta ctctttaaag cgcggatcgg attcgttatt
     6361 gacgatctca aaatcaacgc ccgctttgat ctgcaagccc agtttctgaa tgcgcatttc
     6421 gatcacgttc ggacgaccga taaggatcgg tttcgccagt cccagcgtta ccagttcctg
     6481 agtggcatgc agaacgcgcg cctcttcccc ttccggcaga acaacgcgct tcggcgcttt
     6541 gcgagcctgg gagaaaatcg gcttcataaa caggttggtt ttgtaaacga actcagtcag
     6601 cttgtcgatg tagacgtcga aatcagcaat cggacgagtc gccacgcccg actccatcgc
     6661 ggctttagcg accgcaggag cgatcttaac gatcaagcgc ggatcaaacg gttttggaat
     6721 gatgtattcc ggaccaaagc tcagatcctg atcgccatac gctgaagcca ccacttcgct
     6781 ctgttccgca tgggcgagtt ctgcaatcgc acgtaccgcc gccagtttca tctcttcgtt
     6841 gatggcggtt gcgccaacgt ccagcgcgcc acggaagatg aacgggaagc acaggacgtt
     6901 gttcacctgg ttcggatagt cagaacgacc ggtgcaaatg atggcatccg gacgcacttc
     6961 tttcgccagc ggcggcagaa tttccggttc cgggttcgcc agcgccagga tcattggcgc
     7021 acgagccatt ttcttcacca tttcctgggt cagcactttc gggccggaac agcccaggaa
     7081 aatatccgcg ccttcaatca catcatcgag ggtacgtttg ccgtcatcca ccaccgcata
     7141 tgcggctttg gtttccgcca tgtttggctc acggccctga tagataacgc cttttgaatc
     7201 gcaaaccacg atgttatgtt tttgcagacc cagcgctacc agcaggttca tacaggcgat
     7261 tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg gagatgtttt tctccaccac
     7321 gcgcaagccg ttgaggatgg cggcagtgct gataattgcc gtgccgtgct gatcgtcgtg
     7381 gaataccgga atattcatcc gctcgcgcag tttctgttca atatagaaac attctggcgc
     7441 tttaatgtct tcgaggttga tgccgccgaa ggttggttcg agcgcggcga caacttcaat
     7501 aaatttgtcc gggtcgagtt cgtcaacttc aatgtcaaat acatcaatcc cggcgaattt
     7561 cttaaacaga acgcccttgc cttccatcac cggtttgcct gccagcgcgc caatgttgcc
     7621 taaccccagc accgccgtac cgttagagat caccgccacc aggttaccgc gggcggtata
     7681 tttgtaggct tttaacgggt ctttttcgat ttcaagacaa ggtgcggcaa cgcctggtga
     7741 gtaggccagc gccagatcgc gctgtgttgc cagaggcttg gttggagaaa cctggatttt
     7801 ccctggaact ggaaattcat ggaaatcaag tgcactttgt tttaactggt catccatttg
     7861 gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg gcaacagccc ctgcccacaa
     7921 cgtggcgcac attattaccc tgccggagtc tacagacttt gagcaagtcc aaactctcac
     7981 cattaatata atgttttggt aataatccta taacactgat gttacctgct taatccagca
     8041 ataccatgcc tgtctgctat gcttttttga tgcgtttagc gaaatttctc agaagtgtga
     8101 attaacgcac tcatctaaca ctttactttt caaggagtat ttcctatgaa cgagttagac
     8161 ggcatcaaac agttcaccac tgtcgtggca gacagcggcg atattgagtc cattcgccat
     8221 tatcatcccc aggatgccac caccaatcct tcgctgttac tcaaagctgc cggattatca
     8281 caatatgagc atttaataga cgatgctatc gcctggggta aaaaaaatgg caagacccag
     8341 gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtca atttcggtgc tgaaatcctc
     8401 aaaatcgtac ccggtcgcgt gtcaacagaa gttgatgcac gcctctcttt tgataaagaa
     8461 aagagtattg agaaggcgcg ccatctggtg gacttgtatc agcaacaagg cgttgagaaa
     8521 tcacgcattc tgatcaagct ggcttcgacc tgggaaggaa ttcgcgcggc agaagagctg
     8581 gaaaaagaag gtattaactg caacctgacg ctgctgtttt cttttgcaca ggcacgggcc
     8641 tgtgcggaag caggcgtttt tctgatttcg ccgtttgtcg ggcgtattta tgactggtat
     8701 caggcacgca agccgatgga cccgtatgtg gtggaagaag atccgggcgt taaatcggtg
     8761 cgcaatatct acgactacta taagcaacac cactatgaaa ccattgtgat gggcgcgagc
     8821 ttccgtcgca ccgaacaaat cctcgcctta accggctgcg atcgactgac tatcgcaccg
     8881 aatttactga aggagctgca ggaaaaagtt tcgccagtgg tacgtaaatt aatcccacct
     8941 tctcagacgt tcccacgccc agctcccatg agcgaagcgg agttccgttg ggagcacaat
     9001 caggatgcga tggcggtaga aaaactgtct gaaggcattc gtctgttcgc cgttgatcaa
     9061 cgcaaactgg aagatcttct tgccgccaaa ctataaacca gccacggagt gttatatgtc
     9121 ccgaaaagac cttgccaatg cgattcgcgc actcagtatg gatgcggtac aaaaagccaa
     9181 ctctggtcat cccggcgcgc cgatgggcat ggctgatatt gccgaagtgc tgtggaacga
     9241 ttttcttaaa cataacccta ccgacccaac ctggtatgat cgcgaccgct ttattctttc
     9301 caacggtcac gcgtcgatgc tgctctacag tttgctacat ctgaccggtt acgacctgcc
     9361 gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa accccaggcc acccggagat
     9421 tggctatacg ccaggcgttg aaaccaccac cggcccgctt ggacaaggtt tggcgaacgc
     9481 cgtcgggctg gcgatagcag agcgtacact ggcggcgcag tttaaccagc cagaccatga
     9541 gatcgtcgat cacttcacct atgtgtttat gggcgacggc tgcctgatgg aaggtatttc
     9601 ccacgaagtc tgttcgctgg caggcacgct gggactgggc aagctgattg gtttttacga
     9661 tcacaacggt atttccatcg acggtgaaac agaaggctgg tttaccgacg atacggcaaa
     9721 acgttttgaa gcctatcact ggcatgtgat ccatgaaatc gacggtcacg atccgcaggc
     9781 ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat aagccgtcgc tgattatctg
     9841 ccgtacggtg attggctttg gttcgccgaa taaagcaggt aaggaagagg cgcacggcgc
     9901 accactgggg gaagaagaag tggcgctggc acggcaaaaa ctgggctggc accatccgcc
     9961 atttgagatc cctaaagaga tttatcacgc ctgggatgcc cgtgaaaaag gcgaaaaagc
    10021 gcagcagagc tggaatgaga agtttgccgc ctataaaaag gctcatccgc aactggcaga
    10081 agagtttacc cgacggatga gcggtggttt accgaaggac tgggagaaaa cgactcagaa
    10141 atatatcaat gagttacagg caaatccggc gaaaatcgct acccgtaagg cttcgcaaaa
    10201 tacgcttaac gcttacgggc cgatgctgcc tgagttgctc ggcggttcgg cggatctggc
    10261 tcccagcaac ctgaccatct ggaaaggttc tgtttcgctg aaggaagatc cagcgggcaa
    10321 ctacattcac tacggggtgc gtgaatttgg catgaccgct atcgccaacg gcatcgcgca
    10381 ccacggcggc tttgtgccgt ataccgcgac gttcctgatg tttgttgaat acgcccgtaa
    10441 cgccgcgcgg atggcggcac tgatgaaagc gcggcagatt atggtttata cccacgactc
    10501 aattggcctg ggcgaagatg gtccgacgca ccaggctgtt gagcaactgg ccagcctgcg
    10561 cttaacgcca aatttcagca cctggcgacc gtgcgatcag gtggaagcgg cggtgggctg
    10621 gaagctggcg gttgagcgcc acaacggacc gacggcactg atcctctcaa ggcagaatct
    10681 ggcccaggtg gaacgtacgc cggatcaggt taaagagatt gctcgtggcg gttatgtgct
    10741 gaaagacagc ggcggtaagc cagatattat tctgattgcc accggttcag agatggaaat
    10801 taccctgcaa gcggcagaga aattagcagg agaaggtcgc aatgtacgcg tagtttccct
    10861 gccctcgacc gatattttcg acgcccagga tgaggaatat cgggagtcgg tgttgccttc
    10921 taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc gattactggt acaagtatgt
    10981 tggtctgaaa ggggcaattg tcgggatgac gggttacggg gaatctgctc cggcggataa
    11041 gctgttcccg ttctttggct ttaccgccga gaatattgtg gcaaaagcgc ataaggtgct
    11101 gggagtgaaa ggtgcctgat ggtgattgcc ggatgctgat tgccggatgc gacgctgacg
    11161 cgtcttatcc ggcctacagc gtcttatccg gcctacatgt cccgccattt tgtttaacgg
    11221 gtgatccaca acgtgggcca ggcgtctggc ccatgccagt tatcgcaggt gggttctgcg
    11281 gcgtaacgca ccaggcgaaa acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa
    11341 tcgcttaatc cgcgcccttt cgctaaggtc accagttcac gcgatttctc atcaaatgtt
    11401 gcgttcatca gttccagttc attcgtctcc tgaccgttgt tgaacggcaa acgcaaccga
    11461 accgggcgcg aagctagtgg ctttttacgc gacacaatcc atgccaaatc aatggtgtta
    11521 taggcccctg cctcacagct aatcatcatc agcgctttat catcagtcag cgcagtgaca
    11581 ttcacctcac gacgcaatgg atcaagcgag cagcgcagac cattcatccg ccagtttcca
    11641 taatccagca aatcgttgcg ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc
    11701 gcgacctctt tcagcgcagg cgcgggcggt acgctgagcg gcggttcgtc ccctttcttg
    11761 atccacgcgg tttcactgcc aacgcgcttt tgctgagcat caataaacaa caacgctgct
    11821 tttaagccac tcagagaaat ggtctgatcg ccatcgcgta aggtgattgc cttcccttcc
    11881 tgaatcatct gcaaaaacgc ggtgatggtt gccgtatcat cggttactaa taaccatggt
    11941 gaaatccgcc acttgtcacc acttaacgcc aacggctcgc catctaacag cagccgtggc
    12001 gctatctccc cttctgacgc ctccggcgac ttcaatccgc cgcgctcaat acgtaaaacg
    12061 gcatcggtat gcgccccggc gctgcggctc agggtcatca ccagtccatt atgatcgccc
    12121 gtattacgcg ccacgcagaa attttggtta ttacaggtga cctgccagtc ggaaaacgcc
    12181 cgttgcgctg gtgccgccca caccaaagac gtcggcaaca aagcgaaaaa aaagagaagg
    12241 aaaatgcgat agcgcatgga cggtacgacc ccagaagttc aaacaaacag cacaagtcgt
    12301 atcttcgttg cgcaggtagc gcggctcaat cggatttgtc ggattgagcc aataataaac
    12361 aacgaaatat tattgttatt tttcagtcca ttaaatgtga cgtttgcaaa tagttaagca
    12421 ataacaccgt cttaccgtca cgtatctcgc cggttttgat catctccagc gcctggctga
    12481 acggcagctc gagcacttca atatcttcat cttcgacacc gccaccggcg ttagcgcgct
    12541 gattgtcact gtattcggcg ataaaaaagt ggattagctc agtcacaccg cctggcgaca
    12601 tatacagttc aaataatttg cgcacttcac ccacttcata gcccgtctct tcaatcgctt
    12661 ctttgcgaat acacacttcc ggttcgtcgt tatccagcag cccggcgcag ctttcaatca
    12721 gctgcccgct ttcattgcca ttaacccagg tagcgacacg gaactgacga atcagaacca
    12781 cggtcttttt cttcgtgttg tacaggagga tcgtcgcacc attgccgcga tcgtacactt
    12841 cacgtttatg gcggataact tcgccgtctt tgcgcgtgag atcgtaagta atgttgtgca
    12901 gggtgaaata gttatcggag agaattttgt ctttaatgag ggtgatttgt tgcgtcatac
    12961 cgactccaca gcgcgaaatg aacaattatc ttacgctgtg aagtcgggtt tgtctgcgca
    13021 ggctatcagt gagatttgac tgattttacc cccagccagt caataatccc ctgtgccgca
    13081 tgacgtcctt ctgccattgc ggtaaccacc agatccgcac cacgcacggc gtcaccacca
    13141 gcgaagattt tcggattggt ggtctggtaa cggtactggc tttccacatc cgcgatgatg
    13201 cggccccatt tgtctaccgt tacaccgtgc gactccagcc acggcatccc gtgcggattg
    13261 aagccaaacg ccataatcac cgcgtcggct ggcatgacaa attcactgcc ttccaccggc
    13321 actggacgcc gacgcccctg ggcatccggc tctccaagac gcgtgcgcag gaaacgaatc
    13381 ccgcagacgt gaccttgttc attcagctca agcgccaccg gctggacgtt aaattcgaag
    13441 ttggccccct cttcgcgggc gttcttcact tctttcttcg agcctggcat gttagcttca
    13501 tcacgacgat aagcgcaggt gacgttactc gcgccgtggc gcagtgcggt acgcacacag
    13561 tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa gtccggtggt attgataaac
    13621 ggctcttccg gtagctcttc gagccccatc acctgtttag tgttggcaat gaggaacggc
    13681 agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac ccgctttcat ggaacggtaa
    13741 gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa gcgaatccaa agagacatct
    13801 ttacccactt cacaattgag ttcgaagtga atccccatcg cgctgaagat ttcccgacgg
    13861 cgtgccagca gggatttatc cagtttgaaa gaaggaatgc cgaaagtgag caagccaccg
    13921 atttctggat ggcgatcgta caccgtcacc cccacgccat tgcgggtcag aacatccgca
    13981 caggccagcc ctgccggacc tgcaccgata atcgccaccc gcttgtccac tttggtgaca
    14041 tggcttaagt caggacgcca acctttcgcc aacgcctgat ctgaaatgta gcgttcaatg
    14101 ttgccgatag ttaccgcgcc gtgctcatcg cgaatagtac aggcaccttc acacaaacgg
    14161 tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt tggtctggtg agaaagctcg
    14221 acggcggcgt cgatgtttcc ggctttcacc agttcaatcc actgcggtat atagttatgc
    14281 agcgggcagg tccattcaca aacgctgtgc tcgccgcact taaggcagcg cgaggcttcc
    14341 cgttgtgcct ggtcggcgcg aaatggcaga taaatttcat caaaaccggt tttgcgcgct
    14401 tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg cctgcatttg ttcgacttta
    14461 ctcattaccg gcatttcttg cgccgcggta ctggcatgcc acggttgatg ttcctgacgc
    14521 gcggtgcgca agcggcggga tttcgccatg ccggagagtg cgacgtcagt gaccagttgc
    14581 agcgcgtccg ccgggcaatt ctcaacacag gcaggaccgt tttcgcgccc cgcacaaagg
    14641 tcgcatttat gcgccgtggc ttttactttt cctgccgcga cgggtgtcag gacgatttgc
    14701 atcgtaccaa aaggacaggc caccacgcag gatttacagc caatacactt ttgctgattg
    14761 acctgaatgc tgtcatcaac gtggctgatt gcgccattag ggcagctacg ggcgcagggc
    14821 gcatcttcac aatggtgaca ggtcactgca ctacgttgct gttgatgttt gataaccgta
    14881 attcggggat gaaaatggtg ttggctcagg acatgttgct catcattgtg agccatgaca
    14941 caggcgattt cacaagcatg acaacccaga cattgctgac tgttggccat aataaaacga
    15001 ttcataacga ccttcttttt tggttgtaaa aaccttattc tttatatgag tgttgttatt
    15061 acccgactta caggggaatc ggcaatgttc atgtgcccag aataagtaac tatttcgcta
    15121 taaactgtgg cagatcaaat aatcccatca ctgactaaat tgcgtttcag cgaactggaa
    15181 cattaatgat tttttgtgga gaagacgcgt gtgattgtta aacgacccgt ctcggccagt
    15241 ctggcccggg ccttttttta cattgtgctg ctgtcgattc tttccacggg tatcgctctg
    15301 ctaactctgg cgagcagttt gcgcgacgct gaggctatca atattgccgg atcgctgcgt
    15361 atgcagagtt accgcctggg ctacgacttg caaagtggca gtccacaact caatgcacat
    15421 cgccagttat ttcagcaggc actgcattca ccggtattaa ccaacctcaa cgtctggtat
    15481 gtgccagaag cagtaaaaac tcgctatgcg catctgaatg ccaactggct ggagatgaat
    15541 aatcggctca gcaagggcga tttgccgtgg tatcaggcca atattaataa ttatgttaat
    15601 cagatagacc tgttcgtact ggctttacag cactacgctg aacgcaaaat gctgctggtg
    15661 gtggcgattt ccctggctgg cggcatcggt attttcacgc tggtcttttt tactttgcgc
    15721 cgcatacgcc atcaggtggt tgccccgctg aatcagctgg ttaccgccag tcagcgtatt
    15781 gaacacgggc agttcgactc gccgccgctg gataccaacc tgccgaatga gcttggtctg
    15841 cttgcaaaaa cctttaacca gatgtcgagc gagctgcata aattgtaccg ttcgctggaa
    15901 gcgtcagtag aagaaaagac ccgcgatctc cacgaggcca agcgtcgtct ggaggtgttg
    15961 tatcagtgtt cgcaggcgct gaacactagc cagattgatg tgcattgttt ccgccatatt
    16021 ttgcagattg ttcgcgacaa tgaagcggct gaatatctgg agttaaatgt cggtgaaaac
    16081 tggcggatta gcgaagggca accaaacccg gaattgccga tgcagatttt accggtgaca
    16141 atgcaagaga cggtttacgg cgaactgcac tggcaaaata gtcacgtttc atcatcagaa
    16201 ccgctgctta acagcgtttc gtcgatgctg ggacgcggtt tgtactttaa tcaggcgcag
    16261 aagcattttc agcaattatt gttgatggaa gaacgtgcga ccatcgcccg cgaattgcac
    16321 gactcgctgg ctcaggtact ttcttactta cgtatccagt tgacgttact gaagcgttcg
    16381 ataccggaag ataacgccac cgcacaaagt atcatggccg atttttccca ggcgttgaat
    16441 gatgcttatc ggcagttacg cgagctgttg actactttcc gcctgacgct gcagcaggcg
    16501 gatctcccct ccgcattgag ggaaatgctg gatacgttac aaaatcaaac cagcgccaaa
    16561 ctgaccctcg actgccgtct gccaaccctg gcactggatg cgcaaatgca ggtgcatttg
    16621 ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc acgccaacgc cagcgaaatc
    16681 gccgtcagtt gcgtcaccgc gccggacggc aatcacacgg tttatatccg tgataacggg
    16741 attggtatcg gtgaaccgaa agaacccgaa ggtcattatg gtctgaatat catgcgcgaa
    16801 cgcgcagaac ggctaggtgg gacgctgact ttttcacaac cttccggcgg cggcacgtta
    16861 gtgagtatta gctttcgctc tgcggagggt gaggaaagtc agttaatgta atgcctccta
    16921 ctgaccaaag aatacttgca cttaaggttc agtataaaag ggcatgataa tttacattaa
    16981 ctcctttttt tctccacgat tggctcgtac cttgccgcta cagtgaagca agtcaagcct
    17041 acaacgatac gcagaaacac gaggtcctct tttaatggcg aatttcttta ttgatcgccc
    17101 catttttgcc tgggtgctgg caatcctgtt gtgtctgaca ggtaccctgg cgattttttc
    17161 attgcccgtt gaacaatacc ccgatctcgc gccaccgaat gtgcgagtga ccgctaacta
    17221 tcccggcgca tcggcccaga cgctggaaaa caccgtgacc caggttatcg agcaaaatat
    17281 gaccggcctc gataatctca tgtatatgtc atctcagagc agtggcaccg gtcaggcatc
    17341 tgtcacttta agttttaaag caggcaccga tccggacgaa gccgtgcagc aagtacaaaa
    17401 ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg caaaatcagg gcgtgacggt
    17461 gcgtaaaacc ggcgatacca acattctgac cattgccttc gtctctaccg atggttcgat
    17521 ggataaacag gatattgctg attatgttgc cagtaatatt caggacccgt taagccgcgt
    17581 gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat tccatgcgta tctggctgga
    17641 cccggcgaaa ctcaacagtt tccagatgac ggctaaagat gtcactgatg ccattgagtc
    17701 acagaacgcg cagattgcgg ttgggcaact tggtggtaca ccttccgtcg ataagcaggc
    17761 gctcaacgcc accattaacg cccagtcact gctgcaaaca ccagaacagt tccgcgatat
    17821 caccttgcgg gtcaatcagg acggctcaga ggtaaggctg ggcgatgtcg ccaccgtcga
    17881 aatgggggcg gagaaatacg attatcttag ccgcttcaat ggtaagccag cctccgggct
    17941 gggggtaaaa ctggcctccg gcgctaacga aatggcgaca gcggagctgg tgctcaatcg
    18001 tctcgacgag ctggcgcagt atttcccgca tggactggaa tacaaggtgg cgtatgaaac
    18061 cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa acgctgctgg aagctatcgc
    18121 tctggttttc ctcgttatgt atctgttcct gcaaaacttc cgcgccacgc tgatacccac
    18181 tatcgccgtg ccggtggtgt tgatgggaac cttctccgta ctttacgcct tcggttacag
    18241 cgtcaacacc ttaaccatgt tcgcgatggt gctggcgatc ggtctgctgg tggatgacgc
    18301 catcgtggtg gtggaaaacg tcgaacgtat tatgagtgag gaaggactca ctcctcgcga
    18361 agccacacgt aaatcgatgg ggcagatcca gggggcactg gtcgggattg cgatggttct
    18421 ttcggcggta tttgtaccaa tggccttctt cggcggcacc accggtgcca tctatcgcca
    18481 gttctctatt accattgttg cggcgatggt gctgtcagta ctggtagcga tgatcctcac
    18541 tccggctctg tgtgccacac tacttaagcc actgaaaaaa ggtgagcatc atgggcaaaa
    18601 aggctttttt gcctggttta accagatgtt taaccgcaac gccgaacgct acgaaaaagg
    18661 ggtggcgaaa attctccacc gtagcctgcg ctggattgtg atttatgtcc tgctgcttgg
    18721 cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc ttaccgctgg aagaccgtgg
    18781 catgtttact acctcggtac agttgcccag cggttcaacg caacaacaga ccctgaaagt
    18841 cgttgagcaa atcgagaaat actacttcac ccatgaaaaa gacaacatca tgtcggtgtt
    18901 tgccaccgtt ggttctggcc ctgggggtaa cgggcaaaac gtggcgcgaa tgtttatccg
    18961 cctgaaagac tggagcgaac gcgacagtaa gaccggcacc tcgtttgcca ttatcgagcg
    19021 tgcaacgaag gcgtttaacc aaattaaaga agctcgcgtt atcgccagca gcccgccagc
    19081 aattagcggt cttggtagtt ctgcaggttt tgatatggag ttgcaggacc acgctggagc
    19141 gggtcacgat gcgctgatgg cagcacgtaa tcagttgctg gcgctggcgg cggaaaaccc
    19201 ggagctaacc cgtgtgcgcc ataacggcct cgacgacagt ccgcagttgc agattgatat
    19261 cgaccagcgt aaagctcagg cgctgggcgt tgctatcgac gatattaacg acacactgca
    19321 aaccgcctgg ggttcgagct atgtgaatga ctttatggat cgcggtcgcg tgaagaaagt
    19381 ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat gacatcaatc tctggtatgt
    19441 ccgaaataaa gatggcggca tggtgccctt ctctgctttc gcgacctcac gctgggaaac
    19501 aggctcgccg cgtctggaac gctataacgg ttattctgcg gttgagattg ttggggaagc
    19561 cgcaccgggg gtcagtaccg gtacggcgat ggatattatg gaatcgttag tgaagcagct
    19621 gccaaacggc tttggtctgg agtggacggc gatgtcgtat caggagcggc tttccggcgc
    19681 gcaggctccg gcgctgtacg ccatttcctt gctggtggta ttcctgtgtc tggctgcgtt
    19741 gtatgaaagc tggtcggtgc cgttctcggt aatgctggtc gtgccgctgg gggtaatcgg
    19801 cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac gtttacttcc aggtgggcct
    19861 gttaacggtc attggtttat cggcgaaaaa cgccatcctg atcgtcgagt ttgctaacga
    19921 gatgaaccaa aaaggccacg acctgtttga agcgacgctc cacgcctgcc gtcagcgttt
    19981 acgcccgatt ctgatgacct cgctggcatt tatcttcggc gtattgccaa tggcaaccag
    20041 cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact ggcgtaatgg gcgggatgat
    20101 ttcggccact attctggcta tttacttcgt gccgctgttc tttgtgctgg tgcgccgccg
    20161 cttcccgctg aagccgcgcc cggaataagc aataaaaaag gcgacatgcc aatgtgtcgc
    20221 ctttttcaac tttccgatta agaacctgct cagcgggttc ttgctgtttg tactttgtct
    20281 caggaattac ttacgaagca taacttcgat aaagtctttc cagttcccca gttcacgttc
    20341 aatcataaca acctctctta taattatggg tattctacgg aaacaatata ccgtggtgaa
    20401 gctaatttac tcgattgctg cgatgactac ctccggggga caaatcttat gtaaatacta
    20461 tggtcctgcg gtgatgattt gtatgtgata cacagcaaca tttcgagata ttcatacggc
    20521 atctaatact gatttaattc tggttaaaat a
//
LOCUS       DBBGLQ010000077        20282 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.077, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000077 DBBGLQ010000000
VERSION     DBBGLQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.077"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..224)
                     /locus_tag="ACIYSB_003713"
     CDS             complement(<1..224)
                     /locus_tag="ACIYSB_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; involved in N,N'-diacetylchitobiose
                     transport; protein IIA transfers a phosphoryl group to IIB
                     which then transfers the phosphoryl group to the sugar;
                     IIC forms the translocation channel for the sugar uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGC5430669.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIET"
     gene            complement(309..629)
                     /gene="chbB"
                     /locus_tag="ACIYSB_003714"
     CDS             complement(309..629)
                     /gene="chbB"
                     /locus_tag="ACIYSB_003714"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGC5430670.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(928..1266)
                     /gene="osmE"
                     /locus_tag="ACIYSB_003715"
     CDS             complement(928..1266)
                     /gene="osmE"
                     /locus_tag="ACIYSB_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGC5430671.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1468..2295
                     /gene="nadE"
                     /locus_tag="ACIYSB_003716"
     CDS             1468..2295
                     /gene="nadE"
                     /locus_tag="ACIYSB_003716"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGC5430672.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2525..3412
                     /gene="cho"
                     /locus_tag="ACIYSB_003717"
     CDS             2525..3412
                     /gene="cho"
                     /locus_tag="ACIYSB_003717"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGC5430673.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3372..3947)
                     /gene="ves"
                     /locus_tag="ACIYSB_003718"
     CDS             complement(3372..3947)
                     /gene="ves"
                     /locus_tag="ACIYSB_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGC5430674.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPFQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(4150..4635)
                     /gene="spy"
                     /locus_tag="ACIYSB_003719"
     CDS             complement(4150..4635)
                     /gene="spy"
                     /locus_tag="ACIYSB_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGC5430675.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKVKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4965..5933)
                     /gene="astE"
                     /locus_tag="ACIYSB_003720"
     CDS             complement(4965..5933)
                     /gene="astE"
                     /locus_tag="ACIYSB_003720"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGC5430676.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5926..7269)
                     /gene="astB"
                     /locus_tag="ACIYSB_003721"
     CDS             complement(5926..7269)
                     /gene="astB"
                     /locus_tag="ACIYSB_003721"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGC5430677.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQCVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDTVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(7266..8744)
                     /gene="astD"
                     /locus_tag="ACIYSB_003722"
     CDS             complement(7266..8744)
                     /gene="astD"
                     /locus_tag="ACIYSB_003722"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGC5430678.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSFAERHAVVERFAALLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRPLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8741..9775)
                     /gene="astA"
                     /locus_tag="ACIYSB_003723"
     CDS             complement(8741..9775)
                     /gene="astA"
                     /locus_tag="ACIYSB_003723"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGC5430679.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9772..10992)
                     /gene="astC"
                     /locus_tag="ACIYSB_003724"
     CDS             complement(9772..10992)
                     /gene="astC"
                     /locus_tag="ACIYSB_003724"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGC5430680.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11438..12244
                     /gene="xthA"
                     /locus_tag="ACIYSB_003725"
     CDS             11438..12244
                     /gene="xthA"
                     /locus_tag="ACIYSB_003725"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGC5430681.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12411..13121
                     /gene="ydjX"
                     /locus_tag="ACIYSB_003726"
     CDS             12411..13121
                     /gene="ydjX"
                     /locus_tag="ACIYSB_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGC5430682.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     FFGYSLYILLFIIATLLLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSNTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            13126..13803
                     /gene="ydjY"
                     /locus_tag="ACIYSB_003727"
     CDS             13126..13803
                     /gene="ydjY"
                     /locus_tag="ACIYSB_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGC5430683.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQQENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKIAE"
     gene            13818..14525
                     /gene="ydjZ"
                     /locus_tag="ACIYSB_003728"
     CDS             13818..14525
                     /gene="ydjZ"
                     /locus_tag="ACIYSB_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGC5430684.1"
                     /translation="MMMMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14525..15073
                     /gene="ynjA"
                     /locus_tag="ACIYSB_003729"
     CDS             14525..15073
                     /gene="ynjA"
                     /locus_tag="ACIYSB_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGC5430685.1"
                     /translation="MGLPPLSKIPLILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            15261..16448
                     /locus_tag="ACIYSB_003730"
     CDS             15261..16448
                     /locus_tag="ACIYSB_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5430686.1"
                     /translation="MALTARQVETAKPKDKDYKLSDERGLYLLVKTTGARYWRFKYRI
                     AGKEKKLSFGVYPDVSLAEARTKRDDARKIISEGGDPGEKKRREKLSQKISVTNTFHA
                     LAIEWHHHKSLSWSDSYARSVLEALDKDVFPYLSNRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELSSTLAAPKTAHFPHLRADELPVFLRK
                     LADYHGSPVTRMATNLLILTGLRTIELRLAEWSEIDSSNALWHIPRERMKMRRPHIVP
                     LSRQVISVLQELHGFTGQYRLMLPGRCDVNKPMSEGCINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAHYLDGRRKMMQWYADYIDSLMGK
                     THK"
     gene            complement(16514..16918)
                     /locus_tag="ACIYSB_003731"
                     /pseudo
     CDS             complement(16514..16918)
                     /locus_tag="ACIYSB_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16999..17944)
                     /locus_tag="ACIYSB_003732"
                     /pseudo
     CDS             complement(16999..17944)
                     /locus_tag="ACIYSB_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639406.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(17944..18764)
                     /locus_tag="ACIYSB_003733"
                     /pseudo
     CDS             complement(17944..18764)
                     /locus_tag="ACIYSB_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            19792..19965
                     /locus_tag="ACIYSB_003734"
     CDS             19792..19965
                     /locus_tag="ACIYSB_003734"
                     /inference="COORDINATES: protein motif:HMM:NF025361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebF family protein"
                     /protein_id="HGC5430687.1"
                     /translation="MLLSQQRPVVWVNLRELVSKGDNLVTAHLTARGNKADIQYRVRI
                     DCKNENAIWQRQG"
BASE COUNT         5161 a         5223 c         4836 g         5062 t
ORIGIN      
        1 gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga cccctcccca
       61 aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt taacggcatt
      121 aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat tttaactgca
      181 aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa atacccttaa
      241 aaaccgcaat ttaaatattg cggtattgat ttatgaaata actctttgac gggaaaattt
      301 aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt aagcacgcct
      361 aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt gttgggtaac
      421 aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac gacatcggca
      481 ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac cggaacttca
      541 tatttttctg cctgtgcgcg catttttgat accagtaaag aggtagacat gcccgcagaa
      601 caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat tatctgtcag
      661 ccagacactc cgcaagcctt aacctgcttc catgctctgg gtaacttgcg aaaccaacat
      721 gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt atttctttga
      781 ccttataaat tgtttctcct tgacctgtgg ttatgaccct cttcacattt cgggcaaata
      841 attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc cggtttccgt
      901 tgttactcaa gctttcagac gaattgatta cttcgcagcc tgtggatcag tgtcgtattc
      961 agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg cgacaaagta
     1021 ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac cgcgagcatg
     1081 gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg cccggctcat
     1141 gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac gatcataagc
     1201 cgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg ccatattctt
     1261 gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata cattgggcgc
     1321 tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat gacgttttaa
     1381 ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt
     1441 tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat aaaggcgctg
     1501 ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga ttttctgaaa
     1561 agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag cggcggtcag
     1621 gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg cctggaaacc
     1681 ggcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca ggccgacgaa
     1741 caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac cgttaatatc
     1801 aagggcgcgg tattggccag cgaacaggca ttgcgggaag caggcattga actgagcgat
     1861 tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag cattgcgggt
     1921 atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac cggattcttc
     1981 actaaatatg gtgacggcgg tacggacatt aatccgctgt atcgtctcaa caaacgtcag
     2041 ggtaaacagt tactggcggc attagcttgc ccggaacacc tttataagaa agcgccaacg
     2101 gccgatctgg aagatgatcg cccttctctg ccagatgaag tggcactcgg cgtgacctat
     2161 gacaatatcg acgactatct ggaagggaaa aacgtacctc aacaggtcgc cagaacaata
     2221 gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaccgt tttcgatgat
     2281 ttctggaaaa agtaataatt tgcacatatt ggattgtgcg aaaaagagta atttgttcac
     2341 gccggatgcg gcgtgaacgc cttattcgac ctataaaact atgcaaattc aatacattgc
     2401 aggagtcgaa taggcctgac aggcgtagca cgtcagacgg tgtaaccttt gtcatcgacc
     2461 cgcctctttt ttaatcgctt cccgcctgtt acactggata gataaccagc attcggagtc
     2521 aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg caatttatga
     2581 atatcccgaa catttacgtt cattccttaa tgacttaccc acccgaccag gggtgtatct
     2641 gtttcatggt gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta acatccgcag
     2701 ccgcgtcctt tctcatttac gtaccccgga tgaagccgcc atgctacggc aatcccgacg
     2761 gatcagctgg atatgtaccg ccggtgaaat cggcgctctg ctccttgaag cgcgattaat
     2821 caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc tctgtgccct
     2881 gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc
     2941 ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat tgcagaccat
     3001 cgctgatgaa caaaaacttt gttatggcct gctgggactg gaaccgttaa gtcgcggtcg
     3061 tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta aagagagcca
     3121 tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgggtgg tgtgttggcc
     3181 ttggcaaggg gcggtggcgc tgaaagaaca gcacccggaa atgactcaat atcatattat
     3241 tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg
     3301 gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc tgctttccgg
     3361 taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt gatttcactg
     3421 aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac
     3481 gcgccctggt cggtagtgag caatttatcg cccagttgcc acgccccgtt aatcacaaac
     3541 accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct gactttcgcc
     3601 ttacagacat ccaggcgtgt catgatgttg aaatccatcg acatctgccc tgccgtcagt
     3661 ttcgctttta ccacctggtc cgctgcaaag gcaaaaggct ggaacggttt taaggtatgg
     3721 ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt ccagcaacgt cactatcctt
     3781 tccatgccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat gctggcacgc
     3841 cagtaaaaat cacgttttgc cggtgggaac gtgcaaattt cgcgcgtttc accggcagca
     3901 tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact cgctcccggc
     3961 ccgttctttt cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat
     4021 ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa gaaaaccgcc
     4081 gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg
     4141 gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac gttctgtcag
     4201 acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcagga tgttgtaaat
     4261 tttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt ccatttttgc
     4321 gatctgagct tcagctttta ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc
     4381 gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca tgatttcgcg
     4441 gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt cctgatgcgg
     4501 accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt
     4561 gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa caaacagtgc
     4621 agttaattta cgcatattct atatccttcc tttcagttat ttattacggc tttcttaagt
     4681 agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat cagagcaacg
     4741 gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt
     4801 tgatggagag tgatgagaga atattacaac acgatgattt tgcagagatt atgaagaact
     4861 ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta ttaatttata
     4921 agaaagcaac ttaatacccg cagaatgatt tctgcgggta agtattagct tattttttcg
     4981 agcattaatc ccgcgcgtaa tcccaacgct accaacggat tagggaataa cacatactct
     5041 acatcatggg ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt tcctttctca
     5101 aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg cgagtggcga
     5161 gtaatttgcg aaaccacccg ataacggagc ggcggtgttc tcacgatacc gacactctca
     5221 ccagatagca gcgcagcaat tgcgctggca gttactgcaa actggcgaag atcgttttgc
     5281 ccaaagggca acgctttgcc aagttccagc gtacaggcca gcgcgccaaa atgtctggcg
     5341 ctgaaatggg taaacgtacc accaggttcc tgatggaaca ccagcgcctc cagccccgcc
     5401 gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg
     5461 aactgcggat gcaaggagcc acgaattgcg gtatgtagat caaggtgcca gcgcacagat
     5521 tctttgccct ggtcataaaa atcttccagg cactgttcca gttcgcgcgc ccgacaggtt
     5581 tctccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcgattcat atcgctatgg
     5641 caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag caaccgccaa
     5701 cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat ctccacaggt
     5761 gccgtctcat taccgtgtat tcccgctgaa atcaccagtg cgccttgcgg tggcgttaat
     5821 ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc gaacgccgtt gatttctcgc
     5881 tcggtgataa ccggtttttt acccgttaag gtcagagcaa gaaaattatc cattgccccc
     5941 tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca atacatccag
     6001 tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag taaggcgatc
     6061 gcggtagtaa cgatccaccc agtcattgag cgcattaaac agcgtatcgt tcatcatcac
     6121 cgccggattc accgcccggc gttcttcttc tgtcaatacc acccgcaacc gcaggcacgc
     6181 cgggccgccg ccattcgcca tgctttcacg taaatcaaag acttttagtt cgctaatcgg
     6241 gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgtt cccgacactc
     6301 ctgaggcagc accaacatca tggaaccatc atcgcggctc agcagttggc tgttaaacag
     6361 ataggtagac accgtatcag acacggaaac ctgagttgcc ggaacttcta tcgccataaa
     6421 accattgacc cgcgcacgca ggtttgccag taactgtgac tggcgagcga acgcctgttg
     6481 gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgcactg
     6541 gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattcag
     6601 ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt
     6661 atcattgcct tcttctcgcc cgtagacaaa aagttgcata cccggttcac cgtaatgacc
     6721 gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct gtggcaacgc
     6781 cgaatggacg ctaaatttct cttcgtcgtt aaaaatcgct tttaacagcg attcagtgac
     6841 gggcgcttcc agagaacggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt
     6901 gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga
     6961 agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg
     7021 ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg
     7081 cgggatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg
     7141 cttcgccgcc agtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc
     7201 aaacgacagg cccgcgtaat gatgcgtcag ccctaccagc ccgtcgaaat tgacttccca
     7261 ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt ggcgggcaat
     7321 gttaacgagt ccgactccag gctcgccatc ggccatgcgc agtaatctgc ggcataccag
     7381 gcgctggggc gatggttacc ggaagcacca atgccgccga atggcgcggt actggcagca
     7441 ccggtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg
     7501 aacttttccc gctcggggga aaccagaccg caagagagtc cgaagcgagt gttattcgcc
     7561 attcgaatcg cttcatcgaa agtatcataa cgccagacgc gcaataacgg tccgaacacc
     7621 tcttcatctg gtacgccagc aacgcctgtc atttcaatga tccccggcgt cagcaacgat
     7681 gtccctgctt gtaataagcg cggcgcaagc aggggtcgtc cgcccatcgc ttccagttgc
     7741 tgccatgcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc
     7801 tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagacgagca
     7861 agaaacgcat cgccctgcgc cccgcttttc agcaataaac ggcgggcgca ggtgcagcgt
     7921 tgaccggctg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc
     7981 acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc
     8041 tgaccggaga gctggcgatg caactggtag cctgtattgg cgctaccggt aaacagcaaa
     8101 ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg
     8161 ttcagcacgc ctggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc
     8221 cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg
     8281 atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg
     8341 tgcgggcgat gtcgcaggct cgccgcgccg tccggcattt cactacgctg ctcgccggta
     8401 cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt
     8461 gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa ttcggcttta
     8521 ttgctttcca gcagtgcggc aaagcgttcg acaacggcat gacgttcagc aaatgaaagc
     8581 cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgctcgac ctgagcggca
     8641 tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat
     8701 gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg
     8761 cgcacaggcg caccagacga acgcgatccc cggcgtggca tttgagggca tccagttgtg
     8821 cggcggttaa aatcaaacgc tcggttgccg gatcggtacg caccagcacc acgcggaaat
     8881 ggtgataatt ttcattggcg accaggcagg ctgggaaatc gccctgcgca ggctgccctt
     8941 ctgccacttc caccagccga cttttacgga tggcgcgcac gcggtcgatg tcacactcaa
     9001 gcgtcggccc accgtcaaag atgtcgatat agttacggta gcgaaaacct tctttctcca
     9061 gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct
     9121 gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt
     9181 gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga
     9241 ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca
     9301 ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca
     9361 aatagccgtt gccctcttta cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc
     9421 tgccggtgtg atcgttactg agaaacagcg tcggcaatgc gttatagaca ttcagctctt
     9481 ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg
     9541 ccacctcaat ggcacaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca
     9601 catagccctg ctcacttttg ggcagttcgc cttgccaggt tttgattgcc ctttcgatac
     9661 gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg
     9721 caagctgcat cagcgccgag acatctgatc gctcaacggg acggatgacc atcatgatga
     9781 acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc
     9841 ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacccg caatcagtac
     9901 catcacgcct gctttcgccg cttcctgaga gatctgtttc gcttgcccgg cgtaatcggc
     9961 attcagtaca cagccaatca gcaaacctaa gccgcgaact tcactgaaca aaccatagcg
    10021 gtgattaata gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag
    10081 catctctggt gtgttgatga gctccagcac tttgcctgcc accgccgagg ccagcggatt
    10141 accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc
    10201 caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtagtta acagatcagg
    10261 cgtcacgccg tagtgcatat aggcatacag ttccccggtg cgcccgacgc cggtttgtac
    10321 ttcatcaaaa atcaacagcg cattgtggcg gttacacaat tcacgcagac cttgtaaaaa
    10381 cgcgttgctg gctggcacca caccgccttc cccctggatg ggttcgacaa tcaccgcaca
    10441 ggtagagtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat
    10501 atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt
    10561 aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccgctct tatggctgcc
    10621 gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc
    10681 ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg
    10741 cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt
    10801 cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc
    10861 gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg
    10921 tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat
    10981 tggctgagac atagcgacct ctacagtaaa taattcgtta tttttatgtt aataataagt
    11041 aacgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata
    11101 aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc
    11161 atataaagtg aatacgtttg cgatgtaggt gaataaaaag aataaaaaac gcaatgttat
    11221 gcagaagtaa aatataattc tggaattgtg atcattgacg aaatttactg gaaattactg
    11281 cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatcg ttgacatcat
    11341 taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc
    11401 atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat
    11461 caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga
    11521 tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc
    11581 gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct
    11641 gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca
    11701 gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg
    11761 ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt
    11821 ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat
    11881 tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg
    11941 taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga
    12001 caggctgatg agctgggggt tggtcgatac cttccgccat gcgaatccgc aaacagcaga
    12061 tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat
    12121 cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta
    12181 tgaaatccgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg
    12241 ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca
    12301 acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtct ctgcgttttt
    12361 agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg
    12421 agcgtaaatt tctttttgcc tgtcttattt ttgcgctggt catttacgct atccacgctt
    12481 tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatc cgccagagcg
    12541 gatttttcgg ctatagcctc tatattctgt tattcatcat tgccaccctc ttgctgttac
    12601 caggaagcat actggtgatc gcgggaggaa tagtttttgg cccgctctta gggacactac
    12661 tctcattaat tgccgccacg ctggcctcct cgtgctcatt cctgctggcg cgctggctgg
    12721 gacgtgattt actgctgaaa tacgttggcc atagcaatac cttccaggcc attgaaaaag
    12781 gcattgcgcg taacggtatt gattttctta ttctgacccg cttaatcccg ttgtttcctt
    12841 acaatattca aaattacgct tacggattaa ccacaatcgc cttctggcct tataccctta
    12901 tttcggcact cacgacccta cccggtattg ttatttatac cgtgatggca agcgatctcg
    12961 ccaatgaagg cattacgctg cgctttattt tacaactctg tctggcgggc ctggcgctgt
    13021 ttattctcgt ccagctcgca aaactctacg cccgacacaa acatgtggat ctgtctgctt
    13081 cgcgccgcag cccacttact cacccaaaaa atgaaggata gaacgatgtt gcaacattat
    13141 tcagtgtcat ggaaaaaagg actggctgca ctctgtttac tggctgttgc tgggcttagc
    13201 ggctgcgatc aacaagagaa tgcggcggca aaagtggaat acgacggact ttcgaacagc
    13261 caaccgctgc gtgtcgatgc caataaccat acggtaacca tgctggtgca aattaatggt
    13321 cgtttcctca ccgacgacac tcgtcacggt attgtgttta aagatggctc caacggacat
    13381 aaatcgctgt ttatgggtta tgcgaccccg aaagcatttt atgaagccct gaaagaggca
    13441 ggtggtacgc cgggcgaaaa catgacgatg gataataaag aaacgactca tgtcacaggc
    13501 agcaaactgg atatttcggt caactggcaa ggggcggcaa aagcgtattc cttcgatgaa
    13561 gtgattgttg acagtaatgg caagaaactg gacatgcgct ttggcggtaa tttaacggca
    13621 gcagaagaga agaaaacggg ttgcctggtg tgtctggata gctgcccggt cggcatcgtc
    13681 agcaatgcaa catacactta tggtgcggtt gaaaaacgtg gtgaagttaa attcaaaggc
    13741 aatgcctcag ttctcccggc ggataacacg ctggcaacgg ttacctttaa aatcgccgaa
    13801 taaagccagg ataaaggatg atgatgatgc aatcgcgaaa aatctggtac taccgtataa
    13861 ccctcatcat cctgttgttc gctatgctgc tggcatgggc gctgcttccc ggcgtccatg
    13921 agtttatcaa tcgcagcgtt gcggcgtttg ccgccgtgga ccaacagggt atagaacgct
    13981 ttattcagtc ttacggcgca ctggcggcag ttgtctcgtt cttgttgatg attttgcagg
    14041 ccattgctgc accgctacct gcgtttttga tcacctttgc caatgcgtcg ctgtttggcg
    14101 cgttctgggg cggcttgctg tcgtggacca gttcgatggc cggcgcggcg ctgtgctttt
    14161 ttatcgccag agtgatgggc cgcgaagtgg tggaaaaatt aaccggcaaa accgtgcttg
    14221 acagtatgga cggctttttc actcgctacg gcaaacacac cattctggtc tgtcggttat
    14281 tgccttttgt ccctttcgat ccaatcagct atgctgccgg tttgacttca atacgttttc
    14341 gctcgttttt tatcgccacc gggcttggtc agttaccggc aactattgtt tattcctggg
    14401 cgggcagcat gttaacaggc ggtactttct ggtttgtcac cggactgttt attctgtttg
    14461 ccctgaccgt ggtgattttt atggcgaaga aaatatggct tgaacgccag aagaggaatg
    14521 cctgatgggt ttaccgccgc ttagcaaaat tcctttaatt ttacgtccac aggcgtggct
    14581 gcatcgtcgc cattacggcg aggtgctaag ccccattcgc tggtgggggc ggatcccgtt
    14641 tatcttttat ctggtgtcga tgtttgttgg ctggctggag cgcaaacgct caccgctcga
    14701 tccggtagta cgatcgcttg tcagcgcgcg cattgcgcaa atgtgcctgt gtgagttttg
    14761 tgtggatatc accagtatga aagtcgccga gcgcaccggc agcagcgata aactgctggc
    14821 agtggctgac tggcggcaaa gcccgctctt tagcgatgaa gaacggctgg cgctggagta
    14881 cgccgaagcc gcaagcgtaa cgccgccaac ggtcgatgat gccctgcgta cccgactggc
    14941 tgcgcatttt gacgctcagg cgctcaccga actgacggca ttgatcggcc tgcaaaatct
    15001 gtcagcccgt tttaattctg ccatggacat tcccgctcag gggctgtgcc gtattcctga
    15061 aaaacgttct taaggagggt actaccttca cttctacctc tgcctcccaa actccgtaaa
    15121 actcatgcac aatcaccata ttacagtcaa cccgaacatt atgagctcca ctgaattcta
    15181 ctgttcaccc cctaaatcaa gcataaaatg ggggcatatt tgggggccca ttgtgttcag
    15241 tcagtttcag gagcccccat atggcactta cagccagaca agtcgaaaca gccaaaccta
    15301 aagacaaaga ttataaactc tctgatgaac gtggtttata tctgctggta aaaaccacgg
    15361 gagcacgcta ctggcgcttt aagtacagga tagcaggaaa ggagaaaaaa ctgtccttcg
    15421 gtgtctatcc tgacgtctcc cttgcagaag ccagaaccaa acgcgacgat gcaaggaaaa
    15481 ttatctccga gggcggtgat cccggtgaaa agaaacgaag ggaaaaactc tcacagaaaa
    15541 tctctgtcac caacaccttc catgctctcg caatcgaatg gcatcatcat aaatctttat
    15601 catggtctga cagttacgcc agaagcgtac tggaagctct ggataaagac gttttcccgt
    15661 atctgagcaa ccggagcgtt actgatatcc ttccactgga aatgctggaa atactacgtc
    15721 gaattgaaaa acgaggctct ctggaaaaac tacgcaaggt ccggcaatac tgcaatcaga
    15781 ttttccgtta tgccatagcc acaggacgag ccactgtcaa tccagcctca gaactgagca
    15841 gcacgctggc ggcaccaaaa accgcacatt ttccccatct gagagcggat gaacttcctg
    15901 tttttctccg gaagctcgct gactatcacg gcagtcctgt cactcgtatg gctacaaacc
    15961 tgctgattct gaccgggctc agaacgattg aattgcgact ggccgaatgg tcagaaattg
    16021 attctagcaa cgcactctgg catatcccta gagagagaat gaaaatgcgc cggcctcata
    16081 ttgttcctct gtcacgccag gtcatttctg ttctgcagga attacatggt tttaccggac
    16141 agtatcgcct tatgctaccc ggacgttgcg atgtaaacaa acctatgagt gaaggctgca
    16201 tcaatatggt gctcaaacgt atcggttacg atggcagggc aaccggtcat ggttttcgtc
    16261 acaccatgag taccattctg cacgaacagg gctttaattc tgcctggatt gaaatgcagt
    16321 tagctcatgt ggataaaaac tccatcaggg gtacctataa tcatgcacat tatctcgatg
    16381 gtcgccgtaa aatgatgcag tggtacgctg attacattga ttctcttatg ggaaaaacgc
    16441 acaaatgaaa taaaccatcc cccaccaact gcagcggttg gtgggggctt aactgttatt
    16501 ttttgatttt tgccttcagg cgtgtccaca ggaaaatgca aactgcggta tatacccgtg
    16561 tgataaacaa cagtttaagg ctgaaatact ctcctgcctc gctggcattc gtttcaaaca
    16621 tagcgaaaag aacactctgc gagaactcat gactataaat aaaataataa cagagtgctg
    16681 tcagtgacgt agtccagaga ataataccga caaccgctgc aataattttt atccgttctg
    16741 gaaaaagaaa cacgggtatc cgccacaggg agctgaataa cagtgaatct cgtattccat
    16801 ttattccacc gtatccggtg ctgaagataa tcacctgcag cagggtggaa aagataaagt
    16861 atcgcccccg tgcgctccgg ctgaaagcag gtctgctgtt tccggcattt aaatgtattg
    16921 accatctccc ggtaaaatgt aataaatccc ccgttaccgg aaaaccgatg aacagaattc
    16981 ggacagaaaa tgagttcctc agttacctgt tgcgtttccc ccggtctgat gagccagaga
    17041 cacaatggtc tgtaccgttc tgttgatatt aaaccggtat tcgatatctt ctctggcgct
    17101 ggcaaccatc atcctgaagc gttccgggcg ggacaaaaaa tcccgaagcg cactggtcca
    17161 tgcagtcata tctcccacag gtaaaagtgt tcctgtcaca tttttctgaa tggcatccgg
    17221 gatcccgcca gtctcactgg caataacggg aacaccggag gctgacgctt cagccaggac
    17281 catgccaaat gcctcatttt tcagaaggca tgatagcaca ctggcaaccc ggtatatcgg
    17341 taacgccggg aaaacagcac cagccatgaa aacatcacca ctcattccca gtgattcaat
    17401 ctgcaggcga aggcgttctt cgtattcctc acgaccagcg cccaccacga gccatcgaaa
    17461 tactttccct tccatcttca gttggtgcaa cacccggagc ataaattcat gccctttctc
    17521 gggacgcaac atacccacct ggacaatcag cgggatattg cccgctgatt ctgtccatcc
    17581 ctgaatatgc ggaggtaact gttgcctgac ttctctgtgt aatacagaaa cgatgccagc
    17641 atatcagacg gctcaggcct gcaatgttac tgtcatgacc actgtggcag attaccagat
    17701 caggtctgaa ttaccggatt acctgccgca gtctgatgat ggaaggaagg tgaagactgt
    17761 tcctgaaagg aataaaagta acatcatgtc cgattgttct ggcttcatga gctattttac
    17821 ttttttccct gcaggcgagt aaaacagaat gcccctgttt ctggagcgct gtcatctgtg
    17881 ccagagcctg taactcctgg ccaccaatat ctggtgaaga ctcagtgaat aaaatcttca
    17941 ttattaattc tctggtaatc ttggcccctt atgaagagcc agtaatgaag agaaaaatgg
    18001 cgtgatataa taaaaaggca ccgtttcagc caaccgcaat attcccgttc ttttgtacta
    18061 atacgtccta taagtaatga accttttaca gggcagttca ttcttctttc cgttgtatat
    18121 aaataagata aaccaagttc tctggtgaga ttaatataat ctctgctata atatcctgca
    18181 ggccagcata gatggggact acagtatccc agtttttcct tcagacattg tttgcctgtc
    18241 ataatagctt cttccatgag tcggcactgc tcagtacgtg gtacagacag tctgtcccag
    18301 cgtttatgtg aatgggtatg taaatggaat tcaacaagac cggaatcacg catttcccgg
    18361 acttcagacc agcgaagcat cacttcatcg gaacggttat ctgcaatcaa ctgctcacag
    18421 tcgcgatgcg aatattcccg tttctgtctg taacgcacct tcccgtcacc aatcaaccct
    18481 gtaatgagaa aaatatgcgt atgaagattg tattccttca gtaccggcca tgcccggaac
    18541 cagttatcaa gatatccgtc atcgaatgta atcatgaagc tctttcttgg aagcattccc
    18601 ccctgataaa aatattaaag ttcagcggat gttactgtcc gccagttatt ttttgcgagc
    18661 cactctatct gttcacaaaa tatttcaggt gaaagtgtca caagccccgg acagcgactg
    18721 acatgatggt acatcagtac gggaagatgt cttgcattca acataaagca ttatcaccag
    18781 ttagttatac agtaaatata ccccgagtaa aaacagggcg gtttaggata tgtctggctc
    18841 cgggtattct actatgtaat cagggaataa tggacaatga gaatacaaca gacactttta
    18901 atatttccga ctgtttatca aaataatttt atcctttgtg aatatattgt ttttatcaga
    18961 tgatttactt attttccagg aaaataaaat ccttcgattt caggacgctc agaggaatat
    19021 acctgcttcc gtcaaaaatc ataaaaaccg tgtcccgatt agttttagca gcaggatcaa
    19081 atgcagtaat atcatgatct tataccagta agccactggg aaatgccctc aaaatgtcgt
    19141 gcactgattt ttcggttgag agcctcataa tacctaatat tactggccgt aataatccgg
    19201 aggcacagca tagctttctt gtacagaggc atcatgatga acaggattaa taccgtcaca
    19261 gatgcacaag ttttacccgt gataaagaat cccgttttgt ccgcacttgc ctgcgccagc
    19321 gttgaaaacg tgccggttat tgtagttaag ttattgccgc taactttttc acaacccacc
    19381 tatccggata tttccttatt cttcatatat tacatctcat aaatttaaat gtgagtttct
    19441 catgggtaaa ataaattata cgaatcgcat caataggaaa gacagacggt gctgccgata
    19501 taaaacggga agtgacggga taagcagtta tgtgaatatt gtttcaaaaa atgatatttt
    19561 gtgttgttaa agtataactt tgcaataagc tatccatgat gggattctgt aaatgaacga
    19621 aacactctct tcaattctgc tcatcttcgg tatctgtgca tcatcactgg ctgctgataa
    19681 aaaagaaccg tcagttttcc acgatgtgaa gggcaggaag catcggctgt ttcagccagc
    19741 gtcaaacgga actatctcca gaaacaggtg cttcacagga aagatgacta ggtgctcctc
    19801 agtcagcagc gtcctgttgt ctgggtgaat cttcgtgagc tggtaagtaa aggtgataat
    19861 ttagtcacag cgcatctcac cgccaggggg aataaagccg atattcagta tcgggtgcgc
    19921 attgactgta agaacgaaaa cgctatctgg cagcgccagg gatgaacgct tgaagataac
    19981 cacagagtat gaacaaacgg actctacatg ttcattatga ctaaaaaaac gctgcacgtt
    20041 ctctgtttta ttttacgttg ctgtcataac gtgtttacct caattttcgg gaacgtgttc
    20101 ttagcgctta gactacccct gaatcttctg ctgttgaagg gccgtaacag ggtaagtgat
    20161 aacagatgtc tggacatata ggagcaaatt caccgattta cttaatcatg atgttatttt
    20221 gtaaaataag ctgaggggag tttcacataa tgttagtttt tgtgtaaacg caagaaggaa
    20281 aa
//
LOCUS       DBBGLQ010000078        19402 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.078, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000078 DBBGLQ010000000
VERSION     DBBGLQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.078"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(342..1214)
                     /locus_tag="ACIYSB_003735"
     CDS             complement(342..1214)
                     /locus_tag="ACIYSB_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGC5430688.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGCSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQRGHQQVLFVVTQADKTEPC
                     HEWDVAGIQPSPAQARNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     GHAASPLMTRLQEELRTESVRSQAREQFTGAVDRIFDTAESVCVATVARAVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1595..2080
                     /locus_tag="ACIYSB_003736"
     CDS             1595..2080
                     /locus_tag="ACIYSB_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430689.1"
                     /translation="MTAPEQEEILMKPMVIALNAAIRAGRINLNGKSEDTDSKGFMRT
                     DIDGMPTVINWTDNGYDELRVSVWVDYRPDEVARFRSRYKPDLSPETALPREDRLLFR
                     HFVMICCSCYLERKTGKFIIGTEGNRLFGKYIRDDAPERISDIEDEEPEGYDLFGKVE
                     E"
     gene            2679..3728
                     /locus_tag="ACIYSB_003737"
     CDS             2679..3728
                     /locus_tag="ACIYSB_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGC5430690.1"
                     /translation="MKLFSSKQRTKIYFKRTVIPATFLFASFPAFSQDIVTLYTTREP
                     GLIKPLLDSWTKSSGINVNTVYIKDGMLERIKAEGKNSPADLLMTVDAGNLIDLVEAG
                     VTQPVESEILKTAIPANLRGENNHWFALSMRARVLYAEKNFPIDSLHYEQLASPEYKG
                     RVCMRSGQHPYNTALIAAMIAHHGEEKAEEWLHGLKTNLSRKATGGDRDIARDILGGI
                     CDIGLANSYYVGHMKNAKEGTDARKWGDAIKVIKPAFVQGGTHVNITGAAVARYAPNK
                     ENAIKLMEYLISVPAQQQYAKANFEYPVLKGVDLDPVIRTTIGEIDVDKIPLTDIVKN
                     RKQASLLVDKVGFDQ"
     gene            3745..5400
                     /locus_tag="ACIYSB_003738"
     CDS             3745..5400
                     /locus_tag="ACIYSB_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5430691.1"
                     /translation="MWRYLFSALRSTLKPLHLCSLCIALGVLTPVITLFWLATDADSS
                     QWSGFLTFVLPSALQNTVILLTGVAVMTCVLGVGCAWAVTVWDFPGRKMLSWALVLPL
                     AMPAYIVAFAWLDLLHPLGPLQNFIRLLSGFDSPRQFRLPDLRSLPGAIILLGLVLYP
                     YVYLTCRPVFINQSAHLLEAARTLGCGPVSAFFRVTLPLARPAIVTGMSLALLETLND
                     IGASEFLGIRTITVTVYTTWISRSDLAGAAQIAGIVLTFVFMILGLEFLFRRKQVFSS
                     RSMRGIQQIRIYGWRGWLLCSITALPVILGFCAPVLFLGWESAKRLGEGITISSSLLS
                     ALKNTLFLASGTTLIVSGVSLLVVWSARHSVLNSRFIEFRRTVMHLASSGYAVPGTIL
                     AIGFLPPAMLVDHWLAGLLNISGLPLMSAGILLVICCAIRFLAISTGALDSALGRIPP
                     SLEQASRLLGENGAGTFVRVHLPLLRPALISSALLVFSDVMKELPTTLLLRPVNFETL
                     STLLYAEAARGTYEQGAIAALLIVLAGILPVILLVRRQMNSHH"
     gene            5416..6093
                     /locus_tag="ACIYSB_003739"
     CDS             5416..6093
                     /locus_tag="ACIYSB_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010281453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5430692.1"
                     /translation="MSEIALSLNDVHVVYTRSRQVVLQGFSMSVHKGELVCLLGVSGC
                     GKTTVLRTVAGLERLQQGEIFISQRCVAGAGIHLPPEKRRVGMVFQEYALFPHLTVTQ
                     NVAFGLRHLPYELQHARVTELLKMVELLSCAKRYPHELSGGQQQRIALARALAPAPDI
                     LLLDEPFSSLDKQTSEYLVKEVRDLLRSSGQTALIVTHNESEALEISNRICIMTEGNI
                     SSVQSVV"
     gene            complement(6902..7384)
                     /locus_tag="ACIYSB_003740"
     CDS             complement(6902..7384)
                     /locus_tag="ACIYSB_003740"
                     /inference="COORDINATES: protein motif:HMM:NF018232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGC5430693.1"
                     /translation="MKLPMNLTIALLAGLSCLASWPGINIPVWAIFIGWAWYYALGAT
                     PDILKKIYASLLPGIATSVLCIAAINYMISLHISAMLAIIISVIITVYVLLLLLQIPC
                     MNSSLPAFNAYSTVFAVYYGGFYPDTDGPDISLAVLWAATGLCTGPLLGYISIVCSRK
                     "
     gene            complement(7399..8385)
                     /locus_tag="ACIYSB_003741"
     CDS             complement(7399..8385)
                     /locus_tag="ACIYSB_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HGC5430694.1"
                     /translation="MGIKVLTPGLSTSVQDGGREGFYHLGIPPSGALDRTGLVTANLL
                     VGNHPFDAALECTLLGPELLFEQDTLFAVSGAPFTVTLDNQSVPVNTSLLARKGQILK
                     IAYTTVGVRCYLAIAGGINVPVIAGSRSTYLLGSLGGYKGRRLQAGDELPVQEAICNK
                     GRINYTLPDELRPVFTKENELRVVPGMYIHRLKQESVESFFDDVWKISKDSDRTGFRF
                     QGGRSLNFVDRHPPFGAGSDPSNIVDACYPVGSIQVPSGSTPIVLHRDAVSGGGYMTV
                     GTVVSTDLDILAQLAPGSSVTFSQVSMDKALEIRKEFNERLNLLINSLSYGS"
     gene            complement(8375..9259)
                     /locus_tag="ACIYSB_003742"
     CDS             complement(8375..9259)
                     /locus_tag="ACIYSB_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HGC5430695.1"
                     /translation="MTDINLRYTFGADEHLFIEVSEEMSLKAFFHSLSLTRKIESLNI
                     NGVLDICLANASCQIRFNPDIIHPDDLLQQIRNVNVLSGNGEMTLQTRIIEIPVLYND
                     PWTHETLMRFRNNHQNPALTDLEYAAEINGYPDVQTFIEAHHSSPWFVSMVGFVAGLP
                     FMYQMVDRKKQIQVPKYLVPRTDTPKHSVGHGGCFGAIYPVRGAGGYQLFGITPAPIY
                     DTEQKLSYFKDSMVFFRAGDIIKFRAINTEQYNQCISDVESGNFELLIRPVNFDLNAF
                     EENPEDYTKNLLGELYGN"
     gene            complement(9269..10630)
                     /locus_tag="ACIYSB_003743"
     CDS             complement(9269..10630)
                     /locus_tag="ACIYSB_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019845611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGC5430696.1"
                     /translation="MSIQKLLIANRGEIALRIIRSAKKLNIKTVLACSESDINSLPAK
                     SADEVIVIGKSRADDSYLDGKKIIDAALQSGAQAIHPGYGFLSENASFARAVLDAGLI
                     FVGPSPQIIEQMGDKAIARDCAIKAGVPVVPGSNGEVISPEEALSVASDITGYPLLIK
                     ASAGGGGKGIRIVHSPEQLVHEYTVARAEAKAAFGHDGVYLERYIPEARHVEVQILGD
                     GKNIVHLFERECSLQRKRQKVTEEAPSPSVSASLREALCQSALNLARMLNYSSAGTIE
                     YLVDDRSGEFFFIEMNTRIQVEHPVTEMVTGIDIIDWMLRIAGGDSLSFGQDEITLQG
                     AAIEMRINAENPYNGFMPSPGTISVLQIPEGQGIRVDTHMFEGYKMPLWYDSLLAKLI
                     IHTDSREKTISLARETLGNMQIEGVHTTIPLHEALLSAEFFRNAQFHNKTLEDWLNTG
                     AFK"
     gene            complement(10641..10886)
                     /locus_tag="ACIYSB_003744"
     CDS             complement(10641..10886)
                     /locus_tag="ACIYSB_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HGC5430697.1"
                     /translation="MAVTEILSPLPGVFYRKPAPDSAPFLENGDTVQESTVIGLIEIM
                     KQFNEIHAETTGTNIQFKVENGEEVEPGQVLATIEII"
     gene            complement(10904..11665)
                     /gene="pxpA"
                     /locus_tag="ACIYSB_003745"
     CDS             complement(10904..11665)
                     /gene="pxpA"
                     /locus_tag="ACIYSB_003745"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGC5430698.1"
                     /translation="MLRSIDISSDMGESFGAWTLGDIRDIDIMPYISSANIATGFHAG
                     DPGIMHRTIISARDSGVSVGAHPGYRDLAGFGRRHIHCTAEEITNDMIYQLGALREFA
                     TLHNVPVNHVKPHGALYMHLAHDRDAAKHFVSTLHSYAPELRIYCMYGSAIWKEATQI
                     GHPVICEFYADRDYDQSGSIVFIRRTEPLNPDDVAKKVLRACLEGKVRTVEDQDIDIH
                     FSSVCVHSDTPGALSLLIAINKALREADISVNSQN"
     gene            11808..12743
                     /locus_tag="ACIYSB_003746"
     CDS             11808..12743
                     /locus_tag="ACIYSB_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5430699.1"
                     /translation="MNITLRQIKYFVSTAESGSLSTAAKSLNISQSAITTAIQGLERD
                     IGYKLLIRSKSGVDLTDEGRLFLYNAYNIIRTYDEAINTVHVQKPLHGTVSIAATYTI
                     MGYFLPVHLKQFSRIYPHIKLELFENQRSIIETGLLSGKYDIGIVINDNLTCPDIVSE
                     TLLRSERRIWLPSGHPLLEKEILSLNDIVSEPFIILDVDEAWETAKRYWNTASTMPDV
                     ILHTHSIEAVRSMVANDMGITILSDMVYRPWSLEGKRIETRALSKSEVFPMSIGLAWR
                     NNVEFTPAMQAFINYFRKIFMCSPGYQNRNVDHFI"
     gene            complement(12885..14378)
                     /locus_tag="ACIYSB_003747"
     CDS             complement(12885..14378)
                     /locus_tag="ACIYSB_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGC5430700.1"
                     /translation="MRAFIGDLLLFRMGQEQDKLLHKRLYNALRYAILDGSLPPHSRL
                     PASRELAVELGISRNTVLTTYEQLQAEGYVVSRRGSGTFVAQTAPDTFLMAFSNTEKS
                     EDARLSSAVSHRGDYLLRHVSASPCQWGAFIPGVPDVSAFPHPLFSRIQARISRRPEP
                     ERLSYSCTGGTPELQHALVDYLRIARGVRCQADQILITEGIHQAIDLVARMLADAGDM
                     AWVEEPSYWGIRHVLAMNAVQITPVAVDASGLVPPERLMPPPRLIFVTPSHQYPLGVV
                     MSLERRQRLLALARQHGSWIVEDDYDSEFRFSGQPVPALQGLIDDAPVVYIGTFSKTV
                     YPGLRLGYLVLPRPLKEALKRAHAELYRGGHTLIQMALAEFINGGHYSAHIRRMRLLY
                     SRRRAFLGELIERHCGTQALSDFSDNAGLHLILNLPDDVDDVAVAASANAQNILVRPL
                     SLYYLTAQRKRGLLMGFASLPEAQMEEAFATLLTCLRHHCPQVLVKV"
     gene            14497..15762
                     /locus_tag="ACIYSB_003748"
     CDS             14497..15762
                     /locus_tag="ACIYSB_003748"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGC5430701.1"
                     /translation="MKNSELNHRRQQATPRGVGVMCDYFVGKAENATLWDVEGNEVID
                     FAAGIAVLNTGHRHPKIVAAVEQQLQSFTHTAYQIVPYESYVSLAERINAVAPVDGPA
                     KTAFFTTGAEAVENAVKIARAYTGRPGIITFGGGFHGRTFMTMALTGKVAPYKTGFGP
                     FPGSVYHAVYPNDAHGISVDDALRSLDRIFKADIPADQVAAIVLEPVQGEGGFNVAPA
                     AFMQALRELCDTHGILLVADEVQTGFARTGRLFAMQHYAVKADLITMAKSLAGGFPLS
                     GVVGRAEVMDAPAPGGLGGTYAGNPLAVAAAHAVLDIIEEEQLCQRADQLGEHLKEVL
                     SQARAGCPAIADVRGLGSMVAVEFNNPQSGEPSAEYARQVQHKARENGLLLLNCGIYG
                     NVIRFLYPLTIPDAQFSKALNILTRILRT"
     gene            15897..17141
                     /locus_tag="ACIYSB_003749"
     CDS             15897..17141
                     /locus_tag="ACIYSB_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGC5430702.1"
                     /translation="MSENATFSPEQSCRKHTAAPAKSLSFVEGVSMIVGTNIGAGVLS
                     VAYASGKAGFLPLLFWLVLVGSLTTITMLYVAESTLRTRKHLQLSGLSHRYVGRCGAL
                     IMFLSVCVNSVGALIAYMSGSGKLLHSLLGISPALGSMLFFIPAAGVLYLGLKAIGRG
                     EKFISIGMVVMLTILIVATMLKDTTQMRYLLDGNWLYMVPVFNVVAFCFSAQYIVPEM
                     ARGFSDKPEKLPKAIMVGMGITFILLALVPMSVIALNGLDNISDVATISWGRALGEWA
                     FFSANLFALCAMLTSYWGLGGSFLTNIVDQFSPGDDSQPLRRFLLLLIVVIPPFILAY
                     SGMVSFVNALYFAGVFSGVVLSVMPILILKGARQHGDLTPGWTCPALIIHPLLQSFIV
                     LLYLCSAAYAIASALGHLPAGW"
     gene            17701..18867
                     /gene="lldD"
                     /locus_tag="ACIYSB_003750"
     CDS             17701..18867
                     /gene="lldD"
                     /locus_tag="ACIYSB_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004457 - lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HGC5430703.1"
                     /translation="MIISSPADYRDAAKHRLPRFLFDYIDGGAVTEKTMHENMTSLSS
                     VSLRQQVLCGAGAPELNTTILDSEWAMPVALGPVGATGMYARRGEVQAARAASAMNIP
                     YTLSTVSVCPIEEVAGHADGQLWSQLYVLRDRGYMQNALERAWTAGMKTLVFTVDMPV
                     PGSRYRDNHSGMSGPHATLRQYLQAVLHPRWALDVGIAGRPLSFGNIETYTGHKMSMD
                     DYMGFISKNFDPSIAWNDLEWIRDGWKGKLIIKGILVPEDAKNAVRLGADGIIVSNHG
                     GRQLDGAIPTARALPAIADAVGDDITVLADSGIRSGVDVVRMLALGAKGVLLGRAYIY
                     ALAAAGKKGVEHLLRLYAEDMKVTMTLIGASSPADISSRNLEYLEKEFSRHSSS"
     gene            complement(18944..19234)
                     /locus_tag="ACIYSB_003751"
     CDS             complement(18944..19234)
                     /locus_tag="ACIYSB_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HGC5430704.1"
                     /translation="MGIYHSRVAGIYDTPGSMSDAHFEILAALSPVNSKKVILALRDY
                     FVLGLTRREACENHDVAQGYFSVSLRKLLETNRLVAELAELLSVPSECRPYW"
BASE COUNT         5201 a         4632 c         4427 g         5142 t
ORIGIN      
        1 tcattacatt ctccctaatc aatgatgtaa ataaaaagcc ggtacccaca gtgtgtggat
       61 accggcatgt gtgttattcc gcgcccctgt gaggcgctaa cgctgtggtg gtgtttttct
      121 gccctgaatt tcatggacgg ccgtgtcttt ctgcaggtta ttacaagggt gattattgct
      181 ttgtcgatcg atataaacga tcgataaaat agctacgatc gatatattct attaaccaga
      241 aaggtagtgt gcaagagaat gagttgcata catgactgtt tgcaccatgt tttcatgggg
      301 ttacaaaaat gtaacagcag acaggaacat caccgcaggt ttcagaagaa aatccagttc
      361 cataccgcgc gggcaacaga gaccacggtg tcacgaacag cacgcagaac cgcgcgtgca
      421 acagtggcaa cacagacgct ctccgctgta tcaaatatcc ggtccaccgc accggtaaac
      481 tgttcacggg cctgagagcg gacagactcc gtacgcagtt cttcctgcag gcgggtcatc
      541 aggggactgg cggcatggcc gggaagcgct gtcatgagcg cactgaccag cgtatccagt
      601 tcccagccgg tgcgggccga tacggcaaca accggatgta cgggccggaa cagacggaat
      661 accgcctccg ttttctcgcg aatattccgt gcctgtgcgg gagaaggctg aatgccggcc
      721 acatcccatt cgtggcaggg ctccgttttg tcggcctgcg tcaccacaaa cagcacctgc
      781 tgatgtccgc gttgcaggat gtgtcgccag aaatactcat ccacagacag ggcacggtca
      841 tcggctttaa tcagccacag caccaggtcc agttctggca gaatgtcacg gtacagggct
      901 tcatactctg catccctgtc acggctctcg cccaccccgg gcaggtctgt gataaccatg
      961 ctgcaaccgt ggccgctcag acggaagcgc cgtacttccc gggtgccggc gtgaacatcg
     1021 ctgaccgggg tgacttctcc ctgaaacagt gcattacaga gtgaggattt accggccccg
     1081 cttttaccca taatgccaat cacgggttcg tgactggtga gtttgcgcag atgttcgaga
     1141 atatggcggg aaagtgagta aggcagggag gataggggtt tttcaattgc ctcaatggca
     1201 tcagacggat tcatatatgt gttcccggtg aaaaaagaca aaaccccgcg caccggaaag
     1261 gtggcggggg ttccacggag atgatatctg tctgaaatta tttagaatat cactgtattc
     1321 cggcgtgaca gagtctgatg acggacttat tccgttccgg tcaccggcag gcacatcggt
     1381 aatacgctgt tttattaaag atactgtgat tagagatatg taaataaccg ctcagaatgt
     1441 tctgttatgt cactcaccat gaacagcaca ccgtaacggt gaaagaagaa caagataatc
     1501 aacttccccg tgattgccat cagaaattat tttatccttg acaatattat atgttttatt
     1561 tcggtaaaca ataatggagt gcatcgtgaa aaaaatgact gccccggagc aggaggaaat
     1621 actgatgaag cccatggtga ttgcgctcaa cgcagccatt cgggccggga gaatcaacct
     1681 taacggcaaa tcagaagata cggacagtaa aggattcatg cgtacggata ttgacggaat
     1741 gcccactgtc attaactgga ccgataacgg gtatgatgaa ctgcgtgtat cagtatgggt
     1801 tgattaccgg ccggatgagg tcgccagatt cagatcgcgt tataaacctg atctcagtcc
     1861 tgaaacagcc ctgccccgtg aagacagact gttattcaga cattttgtga tgatttgctg
     1921 cagttgttat ctggagagaa aaacaggtaa gttcatcatt ggcactgaag gaaaccggct
     1981 gtttggtaaa tatattcgtg atgacgctcc ggagagaatt tcagatattg aagacgaaga
     2041 gccggaagga tatgaccttt tcgggaaagt cgaagaatga acggtaagca ggctcagtct
     2101 gatgaaatgg tcaggaccca caggaaagaa gaacacgtct catctcacca caggtcgtct
     2161 gacctgcacg catgacaaaa cgtcatgcgt aagccgatga gtgaaaccgc tgtcaacaga
     2221 attataaagc gaaccgtcca ggatgaccgt gcaacagacc atggattcca tcgcactgca
     2281 ctttatcccc gcccttctgg tgagcactac gccaccggaa ggatgacgca ccgaacagga
     2341 agaataatta ttcttttatt taaagaattt tatataaaac agaaacctcc atactaaatc
     2401 aataaactga gaccttcaca gcatcaccca ccacccacag tgatatataa caaaaaagat
     2461 acaatcacag gcaaatacca cgctgaaaaa cattcagcac aattcaatta cgccagtgag
     2521 taatccggta acagtattac ataccttcct gccagaatct ccacccacat aaattgtcaa
     2581 ataattgcaa aaatttacaa tcaaaacgta ttgaaaataa tttctattac catttaataa
     2641 agtactccct gattacctga ttaagtgagt catcaaacat gaaacttttc agttcaaaac
     2701 agcggacaaa aatttatttc aaacggactg taataccagc aacttttctt ttcgcatcat
     2761 ttcccgcttt ttcacaggat attgtcaccc tttatacaac cagagagccc ggcctgatta
     2821 agccacttct ggacagctgg acaaaaagca gcggtataaa tgttaatacg gtttatatca
     2881 aggacggtat gctggagcgg attaaagctg aaggaaaaaa ttctccagcc gatctactga
     2941 tgaccgtcga tgccggaaat ctcattgatt tagtcgaagc gggagtcact cagcctgttg
     3001 aatctgaaat actgaaaacg gccattccgg caaatcttcg tggagaaaat aaccactggt
     3061 ttgctttatc catgcgggcc cgggtactgt atgcagaaaa aaacttcccc attgacagcc
     3121 tgcattatga acaactcgcc agccctgaat acaaaggcag ggtctgcatg cgttcagggc
     3181 agcaccctta taatacagca ttgattgctg ccatgatcgc ccaccacgga gaagaaaaag
     3241 cagaggagtg gttacacggt cttaaaacca acctgtcacg aaaagcaacg gggggcgatc
     3301 gcgacatagc ccgcgatatt ttaggaggga tatgtgacat cggacttgcg aattcttatt
     3361 acgtcggtca tatgaaaaat gccaaagaag ggacagatgc acgcaaatgg ggagatgcaa
     3421 ttaaagtaat caaaccagcc ttcgtgcagg gagggaccca tgtcaacata accggcgcag
     3481 ccgttgctcg ttatgcaccg aacaaagaaa atgcaataaa actgatggag tatctcatat
     3541 ctgttccggc gcagcagcaa tatgccaaag ctaactttga gtacccggta ctcaaaggcg
     3601 ttgatcttga ccctgtcatc agaaccacta ttggtgaaat agatgtcgat aaaattccgt
     3661 taactgacat cgtaaagaac cgcaaacagg caagcttact ggtagataaa gttgggttcg
     3721 atcaataaat cattttgggc tttagtgtgg cgttatcttt tttctgcatt acgttccacg
     3781 ctaaagcctt tacatctgtg ctcgctgtgt atcgcactgg gtgttcttac acctgtcata
     3841 acgttattct ggctggcaac agatgcagac agttcgcaat ggagcggttt tttaacgttt
     3901 gtgctacctt ccgctctgca gaataccgtt attctgttaa cgggtgttgc tgtaatgaca
     3961 tgcgttcttg gtgtggggtg cgcctgggca gtgactgtct gggattttcc cggccgaaaa
     4021 atgctcagct gggcattagt gttacctctg gccatgcctg cctatattgt tgcttttgca
     4081 tggctcgatc tcctgcatcc cctggggcct ctgcaaaatt ttatccgtct tttatcggga
     4141 tttgacagtc cccgtcagtt tcgtctgccg gatttgcgct cccttcccgg agcgataata
     4201 ttactcggtc ttgtactgta tccttatgtc tacctgacct gccgaccggt gtttatcaat
     4261 cagtccgctc acttactgga ggcagcacga acattaggtt gtggccctgt ctctgctttt
     4321 ttccgggtaa cgctacctct ggcacgccca gcaatagtga caggtatgag tctggccctt
     4381 ctggaaaccc tgaacgacat tggtgcatct gaatttctcg gtatccggac aatcacagta
     4441 acagtctaca ccacatggat atcacgctct gatcttgccg gtgctgcaca aattgccggc
     4501 atagttctga catttgtctt catgattctg ggtcttgaat ttttgttccg gagaaaacag
     4561 gtattcagta gtcgcagtat gcggggaata caacaaatca ggatttatgg ctggcgtgga
     4621 tggttgctgt gcagcattac agcactgccc gttattctgg gattttgcgc acctgtatta
     4681 ttcctgggat gggaaagtgc aaagcgtctc ggagaaggga ttacgatatc ttcatcattg
     4741 ctgtcagcac tgaaaaatac attgtttctc gcatccggta cgaccctgat tgtatcaggg
     4801 gtcagtttac tggtggtctg gagcgcacgg cacagtgtac tgaacagccg tttcatcgaa
     4861 ttccgacgaa cggtcatgca cctggcatct tcaggatatg ctgttcccgg tactattctt
     4921 gctatcggct ttctgccgcc tgcaatgctg gttgatcact ggctcgctgg tcttcttaat
     4981 atcagcggcc ttcccctcat gtctgcaggt atactgttag ttatttgctg tgcgattcgt
     5041 ttcctggcca tttcaaccgg agcgcttgat tcagcccttg gtcgcatacc gccgtcactg
     5101 gaacaggcat cacgattact gggagaaaat ggcgcaggga cttttgtcag agttcatttg
     5161 ccattacttc gccctgctct gatcagcagt gcgctgctgg ttttctccga cgtgatgaaa
     5221 gaactgccga ccaccttact gctacgacct gttaactttg agaccctgtc cacactcctg
     5281 tatgcggaag cggcacgtgg aacctacgaa cagggcgcaa ttgctgcact gttaatcgta
     5341 cttgccggca tactccctgt gattttatta gtacgcaggc aaatgaacag tcaccactaa
     5401 aggaacccaa ccaccatgtc agaaattgcc ctgagtctta atgatgttca tgttgtttat
     5461 accagaagca gacaggtcgt gttacagggt ttttcaatgt ccgttcataa aggtgaactt
     5521 gtatgtctgc ttggcgtttc aggttgtgga aaaacaaccg tattacgaac tgtagcaggc
     5581 ctggaacgat tacaacaagg tgaaatattc atatcacaac gctgtgtagc cggagccgga
     5641 attcatctcc ccccggaaaa gcgcagggtc ggaatggtct ttcaggaata tgctctgttt
     5701 ccccacctga cagtaacaca aaatgtagcc tttggtctgc gccatctgcc ttatgaacta
     5761 cagcatgcca gagtcactga acttctgaaa atggttgaat tattatcctg tgcaaagcgc
     5821 tatcctcatg aactctcagg tggacagcaa caacgtattg cacttgccag agcactggcc
     5881 ccggcccctg atattttgtt actggatgag ccattttcca gtctggataa acaaaccagt
     5941 gaatacctag tgaaagaagt acgggacctg ttacgtagtt caggtcagac agccctgatc
     6001 gttactcata atgaatcaga agcactcgaa atatcaaacc gtatttgtat catgacggag
     6061 ggtaatataa gttcggttca gtctgtggtc tgagacgtaa ccgcacattc aggatcccct
     6121 gttcgtcatg gaagctgtat tatgtaacta tatggaggca tagcagatat tgttgaagaa
     6181 atacatcaaa aacactcaca tttctggtac aacaggagtt gtagatttaa tgattattgg
     6241 gacgaagtta ctacagaaaa gctgtaccgg gaacaggtat ggtagttcct tgtcacgtgt
     6301 ggattaaatg agcataatgc agatcaatta caaaaggcat ttcgtaatag ctctgttgtt
     6361 gattcggtta ccatgatggc tgtggcaaga gccaccatgt gactatacat gatataatta
     6421 tttgtgtgat gttgttatac ctgaacaaca ggcatgagat aatggaaaaa attatacata
     6481 taactcggct ttcccatact ccactacaga acagtatatg attctgaaca tttcaatcca
     6541 ggcgcattac ataactatat tacatgtaac tatctggccc tcaaatcatc gagagaattg
     6601 tttaaagtca gcagtcataa tctgaatata gatcattatc acataattta attcttaatt
     6661 cactacattt tcccttgaat ccggtataat aagacagagc acatggctga tgctttgttt
     6721 cggtgtggat aatctggtgg cgtcggctat gtgctctcaa cagttaactt attatgaata
     6781 ctattatcaa tacaggtttt acataaaaaa ataaaaagca tctcctgata tcagcccagt
     6841 ctctttgggc taaccagaat tatacataag ctatgcagat attaatttgc atacaaaacc
     6901 attattttct tgagcacact atactaatgt atcccagaag aggtcctgtg cataatcctg
     6961 ttgctgccca taatacagca agtgaaatat caggaccatc tgtatccgga taaaatcctc
     7021 cataataaac agcaaataca gtagagtatg cattgaatgc aggtaaacta ctgttcatgc
     7081 acggtatctg aaggagtaaa agaagaacat acactgttat tattactgat ataatgattg
     7141 ccaacatggc agaaatgtgc agggatatca tgtaatttat agcagcaata cataacacag
     7201 acgttgctat accgggaagt aatgaagcat aaattttctt taatatgtca ggcgtcgctc
     7261 ccagagcata gtaccatgcc cagccaataa atatagccca cacaggaata ttaatccccg
     7321 gccatgaagc caggcatgac agccctgcta acagggctat ggtaagattc atcggtaatt
     7381 tcatttcaga cgcacctgtt atgagccata agataatgaa ttaatgagaa ggttgagtct
     7441 ttcattaaat tctttacgga tttccagagc tttatccata ctgacttgtg aaaatgtcac
     7501 actggagccg ggtgcaagtt gagccagaat atcgagatca gtactcacta ctgtccccac
     7561 tgtcatatat ccccctcctg atacagcatc cctgtgcaaa actattggtg tgcttccaga
     7621 gggaacctga attgatccca cgggataaca ggcatcaacg atatttgatg gatctgaacc
     7681 agcaccaaat ggcgggtgcc gatcaacaaa attaagtgag cgccctccct gaaacctgaa
     7741 acctgttctg tctgaatcct tactgatctt ccatacatca tcaaaaaaac tttcaactga
     7801 ttcctgtttc aggcgatgga tatacattcc gggtacaaca cgtaattcat tttctttagt
     7861 aaaaactgga cgtaactcat ctggcagagt gtaatttata cgccctttat tacatattgc
     7921 ttcctgtacg ggtaattcat ctccggcctg taaccgacgt cctttataac caccaagact
     7981 gccaaggagg taagtagaac gacttccggc aataacaggg acattaatac ctcccgcgat
     8041 ggcaaggtaa cagcgtacac ccaccgtggt gtatgcgatt tttaatatct gtcctttccg
     8101 ggctaacagg gatgtattca ccggaacact ctgattatcc agggtcaccg taaaaggggc
     8161 accgctgact gcaaaaagag tatcctgctc aaacaagagt tcgggcccca gtaatgtgca
     8221 ttccagcgct gcatcaaacg ggtgattacc gacaagcaaa ttggcggtaa ctaaccctgt
     8281 acgatctaat gcaccggatg gcgggatccc gagatgataa aagccttctc gtcctccgtc
     8341 ctgaacggac gtggataatc cgggcgtcaa tactttaatt cccatacagt tctcccagta
     8401 aatttttagt ataatcttca ggattttctt caaatgcgtt caaatcaaaa tttacaggcc
     8461 tgataagtaa ttcaaaatta ccagattcca catcacttat acattgatta tattgttcag
     8521 tattgatcgc cctgaactta atgatgtccc ctgcccggaa aaacaccata gagtctttga
     8581 aatatgataa tttctgttct gtgtcataaa tcggagctgg tgttataccg aacaattgat
     8641 acccacctgc cccccggact ggatatatag ccccgaagca cccgccatga ccaacagaat
     8701 gttttggtgt atcagttctg ggaacaagat attttggaac ctggatttgc tttttccggt
     8761 caaccatctg atacataaaa ggcagcccgg caacgaagcc aaccatagaa acaaaccagg
     8821 gacttgaatg atgggcctca ataaacgtct gaacatccgg atatccgttt atttcagcag
     8881 catactcaag atcggttaat gccgggtttt gatgattatt ccggaaccgc atcagggttt
     8941 catgagtcca tggatcatta tacaatacag gaatttcaat tatacgtgtc tgtagtgtca
     9001 tttctccatt cccggagagc acattaacat ttcgtatctg ttgtaacaaa tcatcagggt
     9061 gtataatgtc gggattaaat cttatctgac atgatgcatt tgcaaggcaa atatcgagca
     9121 caccattaat atttaatgat tctatttttc tcgtaagaga caagctgtga aaaaatgctt
     9181 taagagacat ttcctcactg acttcgatga aaagatgctc atcagcacca aatgtatagc
     9241 gcaaatttat atcagtcata caggttcctc atttaaatgc accggtattc agccagtctt
     9301 caagcgtttt attatgaaac tgagcatttc tgaaaaactc agctgataat aatgcttcat
     9361 gaagaggtat agttgtatga acgccttcta tttgcatatt accaagggtc tcccgggcaa
     9421 gacttatcgt tttttctctg ctgtcagtat gaataataag ttttgccagt aaagaatcat
     9481 accagagagg catcttatac ccttcaaaca tgtgagtatc gactcttatt ccctgccctt
     9541 caggtatctg caataccgat attgttccgg gactgggcat aaaaccattg taaggatttt
     9601 ctgcattaat cctcatttca attgcagctc cctgtaacgt aatctcatcc tgtccaaacg
     9661 acagagagtc gcccccggca attctcagca tccagtcgat aatatcaatg cctgtgacca
     9721 tttctgtaac cggatgttca acctgaatcc gcgtattcat ttcaataaaa aagaattccc
     9781 cgctccggtc atcgaccagg tactcaatag ttcctgcgct tgaataattc agcattctgg
     9841 cgagattcag tgcactctga cataaggcct cacgtagtga cgctgaaaca gaaggcgatg
     9901 gcgcttcttc tgtcactttt tgtctttttc tctgcagaga acactctcgt tcaaacaggt
     9961 gaacgatgtt ttttccgtct ccaaggatct gaacttcaac atgacgggct tccggtatat
    10021 aacgttccag atatacacca tcgtgcccga aagctgcttt tgcttcagca cgcgctactg
    10081 tatattcatg caccagctgt tccggagaat gtacaatccg tatccccttc ccgcctcccc
    10141 cggccgaggc ctttatcaat aaaggatatc cggtaatatc cgatgctact gataatgcct
    10201 cctcagggct tataacctcg ccatttgagc ctggtacaac cgggacacct gcctttatcg
    10261 cgcaatctct cgcgattgct ttatccccca tttgctcaat aatctgagga gatggcccaa
    10321 caaaaataag ccctgcatcg agcaccgcac gggcaaaaga cgcattctcc gacagaaaac
    10381 catatccggg atgaatggcc tgagcgccag actgaagggc tgcatcaatt atcttctttc
    10441 cgtccagata actgtcatcg gcacgggatt tacctatgac aataacttca tcggcgctct
    10501 ttgccggaag agaattgatg tctgattcgc tacatgccag caccgttttt atattcagct
    10561 tctttgcact tcgtataatt cgtaatgcaa tctcacctct gttggcaata agcagttttt
    10621 gaatactcat gctcagttcc ttatattatt tctatggtag ccagtacctg accaggttcc
    10681 acttcctccc cattctccac cttaaactgg atattagtcc cggttgtttc agcatgaatt
    10741 tcgttaaact gcttcattat ttcaatcaac ccaatgacgg tactctcctg aacagtgtca
    10801 ccattctcca gaaacggggc actatcaggt gctggtttcc gataaaatac accaggtaaa
    10861 ggtgaaagaa tttctgtaac agccataaca tcctccatgt atatcagttt tgagaattta
    10921 cacttatgtc tgcctcacgt aaggctttat ttattgcaat cagaagagat aaggcaccgg
    10981 gcgtatcact atggacacag acagaagaga aatgaatgtc aatatcctga tcttctacag
    11041 tacgaacttt tccctccaga caggcacgca gcactttttt tgcgacatca tcaggattaa
    11101 gaggttcagt tctccgtata aatacaatgg aaccgctttg atcataatcc ctgtcggcat
    11161 aaaactcaca aataacagga tgtccgattt gcgtcgcttc tttccagatg gcactaccat
    11221 acatgcaata tattctcaat tcaggcgcat aactatgaag cgttgagaca aaatgctttg
    11281 cagcatcccg atcatgagca agatgcatat acaacgcgcc atgaggctta acatggttta
    11341 caggcacatt gtgtaatgtg gcaaattcac gcaaggcccc cagctgataa atcatgtcat
    11401 tggtgatttc ctctgcggtg cagtgaatat gacgccggcc aaaaccagcc agatcacgat
    11461 aaccgggatg agcaccgaca gacacaccac tgtcacgggc gctgattatt gtacggtgca
    11521 taatccccgg atcacctgca tgaaaacctg tcgctatatt cgctgaggag atgtatggca
    11581 taatatcaat atcccggata tctccgagag tccatgctcc aaagctttcc cccatatcag
    11641 agctgatatc aatactgcga agcattaaaa cctccgtctg acaaatacgg taacagtatg
    11701 tcatgtagca catttgtttc tttattgtaa attattattt ttctatggta atatattaaa
    11761 tttatttata attactgttg caaaaatatc tttatcagga aaatttgatg aacatcacac
    11821 tcagacagat aaaatatttc gtatcaacag cggagtccgg atcgttatca actgcagcca
    11881 aatcactcaa tatttcacag tctgcgataa caacagcaat tcaggggctt gaaagggaca
    11941 tcggttataa attactgatt cgctcaaaat ctggcgttga tttaacagat gagggaagac
    12001 tgttcttata caatgcctac aatattatcc ggacatatga tgaggcaata aatacagtgc
    12061 atgttcagaa gcctttgcat ggaacagttt ccattgcggc gacttataca ataatgggat
    12121 attttttacc tgttcatctg aaacaatttt caagaattta tccacatata aagctggagc
    12181 tttttgaaaa tcaaagaagc attattgaaa ccggacttct cagcggaaaa tatgatattg
    12241 ggattgtaat caatgataat ctgacatgtc ccgatattgt atcagaaaca ctgttacggt
    12301 cggagcgccg tatatggtta ccatccggcc accctctgct ggaaaaagaa attctctccc
    12361 tgaatgatat tgtctcagag ccttttatta tccttgatgt ggatgaagca tgggaaacag
    12421 caaaacgtta ctggaatacg gccagtacca tgccagatgt aattttacat acgcattcta
    12481 ttgaagctgt acgaagtatg gttgcgaatg atatgggtat aaccattctt tctgacatgg
    12541 tttatcgccc atggtcgctt gaagggaaac gtatagaaac acgcgcatta tctaaatcag
    12601 aagttttccc aatgagtatt ggcctggcat ggcgtaataa tgttgagttt acgcctgcaa
    12661 tgcaggcttt catcaattat ttcagaaaga tttttatgtg ttctcccggt tatcaaaacc
    12721 ggaatgttga ccactttatc tgatgtgtta aagacatcac tttattatta ccactatagc
    12781 tgaactcatt gaagatgatt aactactgtc tgctcagaga aacgatgtaa agtgaaatgt
    12841 gaatactcct gaccagagtg ttgttccctc tgttaccagc cacctcagac ctttaccagc
    12901 acctgagggc agtgatggcg caaacaggtc agaagcgtgg cgaaagcctc ttccatctgc
    12961 gcttccggca gactcgcaaa tcccatcaac agtcctcttt tacgctgtgc tgtcaggtag
    13021 taaagcgaca gcggacgcac cagtatattt tgtgcattgg cgctggccgc aaccgcaaca
    13081 tcatcaacat cgtccggtaa atttaatatc agatgcagcc cggcattatc gctgaaatct
    13141 gacagtgcct gtgttccaca gtgacgttca atcagttcac caagaaaggc gcgacggcga
    13201 ctgtacagta accgcatacg gcgaatatga gcagaataat gccctccgtt gataaactca
    13261 gccagtgcca tctggatcag tgtatgcccg ccacgataaa gctcagcatg ggcacgtttt
    13321 aaagcctctt tcagcgggcg cggtaatacc agataaccca gcctcagtcc gggatacact
    13381 gtcttgctga aagtaccaat atacaccact ggtgcatcgt caataagccc ctgtagtgcc
    13441 ggaactggtt gaccggaaaa gcgaaattca ctgtcataat catcttccac aatccagctt
    13501 ccatgctgtc tggcaagggc cagcaagcgt tgccgtcgct caagactcat caccacgccc
    13561 agagggtatt gatgggatgg cgtgacgaaa atcagcctgg gaggtggcat caggcgttcg
    13621 ggaggtacca gaccactggc atcaacggca acaggggtga tctgcacagc attcattgcc
    13681 agtacatggc gtattcccca gtaagacggc tcttccaccc atgccatatc accggcatct
    13741 gccagcatgc gtgcaacaag atcgatcgcc tgatggatac cttcggtgat aagtatctga
    13801 tcagcctgac agcgtacgcc acgcgcaatc cgtaaataat caaccagtgc atgctgtaat
    13861 tccggtgtcc ctccggtaca gctatagctc agccgctccg gctcaggacg gcgactgata
    13921 cgcgcctgaa tcctgctgaa cagggggtgg ggaaaggcac tgacatccgg gacgccggga
    13981 ataaatgccc cccactggca cggactggcg ctgacatgcc ggagcagata atccccgcgg
    14041 tgagaaacag cagaactcag cctggcatct tcactttttt ccgtgttact gaacgccatt
    14101 aagaaggtat caggagccgt ctgtgcgacg aatgttccgc ttccacgacg tgataccaca
    14161 tatccttccg cctgtaattg ttcataagtt gttaatactg tgtttcttga gattccaagt
    14221 tcaactgcaa gttccctgga ggcagggaga cggctgtgcg gcggtaaaga gccatcgaga
    14281 atagcatagc gaagcgcatt atagagccgt ttgtgcagaa gtttatcctg ctcctgaccc
    14341 attctgaaca gcagcagatc gcctatgaat gccctcaact ggatccctca gataatcaaa
    14401 attggtactc gattatagtg ccatcgcctg cctaaataaa caacacagcg tctcatttgt
    14461 tgcagttaag tgacatattt aacaggagag tcgggaatga aaaattctga actgaatcat
    14521 cgtcgccagc aggcgactcc ccgtggtgtg ggggtaatgt gtgactactt tgtcgggaaa
    14581 gcggagaacg ccacgctgtg ggatgtggaa ggcaatgaag ttatcgactt tgccgccggt
    14641 attgccgtac tgaacaccgg ccatcggcat ccaaaaattg tggcggcggt agagcagcag
    14701 ttgcagtcgt ttacccatac cgcttatcag attgtccctt atgagagtta cgtttcgctt
    14761 gcagaacgta ttaatgctgt cgcccctgtc gatggacctg ccaaaacggc attttttacc
    14821 accggtgccg aagcagttga aaatgcggtg aaaattgcgc gcgcatacac cggtcgtccg
    14881 gggattatta cttttggcgg tgggttccat ggtcgtacat tcatgaccat ggcactcacg
    14941 ggcaaagtcg ctccctacaa aacagggttt ggcccgttcc cgggctcagt ttaccacgcc
    15001 gtctatccta atgatgccca tggcatttcc gttgatgatg ctctcaggag tcttgatcgt
    15061 atctttaaag cagatattcc tgctgatcag gtcgccgcga ttgtactgga gcccgttcag
    15121 ggagaaggcg ggttcaatgt cgctccggcc gcttttatgc aggcattgcg tgagttgtgc
    15181 gatacacacg ggatcctgct ggttgccgac gaagtacaga ccggttttgc ccgtactggc
    15241 aggctatttg cgatgcagca ctacgctgtg aaagccgatc tgataacaat ggcgaaaagt
    15301 ctggctggcg gcttcccgtt atcgggggtt gtggggcgtg ccgaagtcat ggatgccccg
    15361 gctccgggag gactgggagg tacttatgcc ggtaatccgc tggcagtagc tgctgcgcat
    15421 gcggtgctgg atatcattga agaagaacag ctctgccagc gtgccgatca acttggcgaa
    15481 catctgaaag aggtgctcag tcaggcccgg gcaggatgtc cggcaattgc ggatgtgcgt
    15541 ggactgggtt caatggtggc agtggagttt aacaatccgc aaagcggtga gccgtcggcg
    15601 gaatatgcac gacaggtaca gcacaaagcc cgggaaaacg gcctgctgct gttgaactgt
    15661 ggtatctacg gtaacgttat ccgttttctc taccctttaa caatcccgga tgctcagttc
    15721 agcaaagcgc tgaatattct gacccgaatt ctgcgtacct gatatgcacg gaaaattgcc
    15781 acaacggcag ggaagccttt ctttcccacg tgtcgtttgt gccaggcgtg agtgatttat
    15841 tcctgatatc acctttttcc ggcggcaata gtcgccggtg taatacggaa tctgttatgt
    15901 ctgaaaatgc caccttttcc cctgaacagt catgccgcaa acataccgct gcgccggcga
    15961 aatccctgag tttcgttgaa ggtgtgtcga tgattgtcgg cacgaacatt ggcgcaggtg
    16021 tactgtctgt ggcctacgcc tccggtaaag ccggttttct gccgctgtta ttctggctcg
    16081 tgctggtcgg gagcctgacc accataacca tgctgtatgt ggctgaatcg actttacgta
    16141 cccgtaaaca tttgcagtta agcggattgt cacatcgtta tgtcggacgc tgtggcgcgc
    16201 tgataatgtt cctttccgtc tgtgtgaaca gcgttggcgc tctgattgca tatatgagcg
    16261 gaagcggtaa gctgctgcac tcactgctgg gcatttctcc agctctgggt agcatgttat
    16321 ttttcattcc cgccgcagga gtgctttatc tcggcctgaa agcgataggt cgcggggaaa
    16381 aatttataag tatcggtatg gtggtgatgc tcactatcct tatcgtcgcc acaatgctga
    16441 aagacacaac acaaatgcgt tacctgctgg atggcaactg gctgtatatg gtgccggtct
    16501 ttaacgttgt ggcattttgt ttctcggcac aatatattgt tccggaaatg gcgcgcggtt
    16561 tttctgataa accagaaaaa ttgccgaagg caattatggt cgggatggga attaccttca
    16621 ttctgctggc tctggtgcct atgtctgtca tagcactcaa cgggctggac aatatttctg
    16681 atgtggcgac catttcctgg ggacgtgcgc tgggtgagtg ggcctttttc tctgcgaatc
    16741 tgtttgctct ttgcgcgatg ctgacttctt actggggact tggtggcagt tttctgacca
    16801 atatcgttga ccagttcagt ccgggcgatg acagccagcc tctaagacgc tttctgttac
    16861 tgctgatcgt tgtcatacca ccgtttattc ttgcttatag cggcatggta tcgtttgtaa
    16921 atgcgcttta ttttgcgggg gtcttcagtg gcgtggtcct gtctgttatg cccattctga
    16981 tactgaaagg ggcgcgccag cacggtgacc tgacacctgg ctggacgtgt cctgcactca
    17041 ttattcatcc gctactccag agctttattg tgttgttata tctctgtagc gctgcgtatg
    17101 caattgcttc tgcgctgggc catctcccgg caggctggta aacataaagg aaaagtatta
    17161 tcgacctcct gtatattaaa attgagttag catagtgacg aacagatttg gcagggcata
    17221 tgtagcctgc tcccctccgg ggtttaaagc tctttttgta agcaacgggc gcatgtgctg
    17281 acccactcat ttgacatcat gacggtttat ctgtacgtca ccataacgca ttttataaga
    17341 atgttttttg tcccataaca ggtgtccact ggttaaaaag gtgggcacct gtcgttacca
    17401 gtactggctt aattgctaca tacggctgtc ggtttacgct tactttcaac ttgtgggtaa
    17461 ggggatggag tgcgaataaa tgatcgagct tcgtgcgaat cactgatcga atacatcgga
    17521 atgcgcactc tgaagcaaac ttaaaacatc tctgcaccat tccatgcacg cataaaagtc
    17581 attccggcat ttcattttac ataacataaa cagaacaata ataattacaa caacattcac
    17641 accagcatta cagaacacac tctgtctgtt gttatcacca gaaatatcag gagtatttaa
    17701 atgattattt cctcacctgc agattacagg gacgccgcaa aacacagact gccacgcttt
    17761 ctttttgatt acatagacgg cggggctgtt actgaaaaaa ccatgcatga aaatatgacc
    17821 tctttatctt cggtcagcct ccggcagcag gtgctttgtg gcgccggagc ccccgaactg
    17881 aacaccacca tccttgattc agaatgggcc atgcctgttg ctctgggccc cgtcggtgcc
    17941 actggcatgt acgcccgtcg tggagaggtg caggcagcca gagcggcctc agcaatgaac
    18001 atcccttata ctctctcaac cgtctccgtc tgcccgatcg aagaggtcgc cggacacgct
    18061 gacgggcaac tgtggtccca gttatacgtc cttcgggatc gtggttatat gcaaaacgca
    18121 cttgaacgcg cctggactgc aggtatgaaa accctggtat ttaccgtcga catgcctgtg
    18181 ccgggctccc ggtacaggga taaccattca gggatgtcag gtccccacgc cacactgcgt
    18241 cagtatcttc aggccgttct tcatccccgc tgggcgctgg atgtgggaat tgccggtcgt
    18301 cctctgtcat ttggtaacat cgaaacgtac accgggcata aaatgtcgat ggatgactat
    18361 atgggcttta tcagcaaaaa ctttgatccc tctattgcat ggaacgatct cgaatggatc
    18421 cgtgatggct ggaaaggtaa actgataata aaagggatcc ttgttccgga agatgcaaaa
    18481 aatgctgtac ggcttggcgc ggatggcatc attgtctcca atcatggcgg acgtcagctt
    18541 gatggtgcca tccccacggc cagggcatta ccggcgatag cagatgcagt gggtgatgac
    18601 atcacggtac tggcggattc aggtattcgt tccggtgtgg atgtggtccg gatgcttgct
    18661 ctgggggcaa aaggtgtgct gctgggaaga gcgtatattt acgcacttgc cgctgcggga
    18721 aaaaaaggcg ttgagcacct gctcagactt tatgctgaag atatgaaagt caccatgacg
    18781 ctgatcggcg cttcttctcc cgctgatatc agcagcagaa atcttgaata tctggaaaaa
    18841 gaattctccc gacattcatc atcctgacca aagtaaacca ggcacatatc ctccatgctc
    18901 ccgggacaac agagcgcagt tcgggcacta cagccttact ggtttaccag tatggtctgc
    18961 attcagaagg gaccgacaac aactcagcca gctccgccac aaggcggttg gtttccagta
    19021 atttccgcag actcaccgaa aaatagcctt gtgccacatc gtgattttca caggcttccc
    19081 tgcgcgtcag ccccagaaca aaataatccc tcagcgccag gataaccttt ttactgttaa
    19141 cgggagaaag cgctgcaagt atttcaaaat gtgcatctga catgcttccg ggcgtgtcat
    19201 atatcccggc aacacgactg tgatatatcc ccacggatac cccctgatta ccgcctgccc
    19261 ttattccccg ggagactgag aacaggctga gatataagta tatttatata atattatatt
    19321 tcggattatt cgcagaatac tgaaaaaacc ggtcgggtca accgcaggaa aacagaaaga
    19381 gcctgtaata acgcatagaa ac
//
LOCUS       DBBGLQ010000079        19336 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.079, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000079 DBBGLQ010000000
VERSION     DBBGLQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.079"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            260..913
                     /locus_tag="ACIYSB_003752"
     CDS             260..913
                     /locus_tag="ACIYSB_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HGC5430705.1"
                     /translation="MEKIAIFSLTTSKPQMLTAILKDGALIINEVKPLPASALEQKQK
                     IPPAIAALRKSKFKVLVDEITPTISAGTGASQVTLKTRHADGRAAIIVGMERYRELKL
                     QKLLSLPQNNKGAFEIPDSIVDTEYNGNGEEVYRVNWQDIRPEHILMILCCYATVYHN
                     VASADYVEQMTGTVEKEQKTGILASFLSIIGHEKVEAGTSQPKSLAGKEVDEDTVIL"
     gene            1242..1571
                     /locus_tag="ACIYSB_003753"
     CDS             1242..1571
                     /locus_tag="ACIYSB_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGC5430706.1"
                     /translation="MGYSRLDDRYIEDDIFRALFHQEMIKRVSEYHSDNFQYTIKIDE
                     VYRSDLAAYRAYGNADLRWVFRVLVGHESEMEEMPAGTTLTLPDVAWLRNKIRDYASA
                     EPEIENA"
     gene            1564..2760
                     /locus_tag="ACIYSB_003754"
     CDS             1564..2760
                     /locus_tag="ACIYSB_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5430707.1"
                     /translation="MPDFLKNQDGRYITDGLSSKDFTRLFDLISKEQTRKRRQAHRTL
                     TPGRLRNKSAEDILKLGKKKGGTFFTRDDLKGFEKLRSKTREKYDSKTAGITYAQLVA
                     SSQAIDIKRANNAVDDGSGIKRATPVSLRHNVINIRVEASDISVHQHHIVRIRFEEWD
                     QMVDDIAEDDKSALKITKSLCAGRVSFDCDCGRHQYWYRYIATAGNFALAPPKEYAYP
                     KVRNPKLQGVACKHVIHSMTRLQSASWQMSIARALQKAATQIAFGDDRRRTTKHFSKE
                     DEKEFNRNRSSKTNVEAAKREWRLYQKRQAALSTKLAKDSGKIDKLRDQLTRARKLSD
                     AQKKRAAAKEAALQREKQKNKELQQRLADQFALKKQAFIDALVMAGTSPEQAEKMFME
                     YVKKGS"
     gene            complement(2795..3154)
                     /locus_tag="ACIYSB_003755"
     CDS             complement(2795..3154)
                     /locus_tag="ACIYSB_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5430708.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEMNTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIKFRMEQQGLTVKDLEPAIGKSNRVYEVLNGTRNLTLPMI
                     RRLHSQFGIPLESLVGV"
     gene            complement(3158..3460)
                     /locus_tag="ACIYSB_003756"
     CDS             complement(3158..3460)
                     /locus_tag="ACIYSB_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HGC5430709.1"
                     /translation="MKIISVKTLRDFWAKNPDAEQPLKAWVDEATKADWKNPAEIKEQ
                     YRTASILKNRRVVFNIKGNDYRLIVAIAYQRGWMFVKFIGTHKEYDAIEADTIELE"
     gene            3606..4397
                     /locus_tag="ACIYSB_003757"
     CDS             3606..4397
                     /locus_tag="ACIYSB_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5430710.1"
                     /translation="MGRFDEWFADDANLASKESEHDSTQRETPATVEADAGGAPEHGN
                     TGSDSFSILEEQPAAKADSHDNFADSNGGLTPDAGESDIAILPSSLAGREPTPQLKAR
                     YNGHKAFNDQIRADWMLIIESSPDAFQALLYRPDVGTYGVVSDETGEESFTELDNNQR
                     ELTYQEPEIVYVLDNPDGRDSFHAIDADGEQDGLTDDVLILRIAANNVPVGSILEWNE
                     EMVNGVARRWWYVHRIFSYGTQHVGSLYYCIPARNFDTTQNGVIV"
     gene            4394..5161
                     /locus_tag="ACIYSB_003758"
     CDS             4394..5161
                     /locus_tag="ACIYSB_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HGC5430711.1"
                     /translation="MTSNKTLLARTGEWQTSRTGKFQTTGFENVDNAFATLISNIFSD
                     ILLVAPAPEEKRFASFLNRPPAERVYVARFDNAIEFLKAVRRANAGQGRKPENQNINR
                     DALPLVNISRNMDINYINDDQQIDRKKVASFCEPDTGMPLAELEYTQAILTYDVTLMA
                     TDKATMSLMCNSLGARLRLMTGTQFEATTHLVRVPVPLICSIQDAKEVGFTDVSAPIG
                     EERIYATQAPISVIADVITAWELDAKRIITETSISMG"
     gene            5165..6145
                     /locus_tag="ACIYSB_003759"
     CDS             5165..6145
                     /locus_tag="ACIYSB_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail length tape measure protein"
                     /protein_id="HGC5430712.1"
                     /translation="MAQELQQYFLQSVLINDNKVPRDWIFTAVYVEKTSLKAPLLKLE
                     IHDATGTVIDDWKAKYGASLVAEMGDPNGNAGTFKTDFFVTSAMLAGDVVTVIAVSED
                     VRRFKIPSPRTNLHTNKTPDAIFKAYSGKLKITSSVLKRAVTYHLNAGDKPSKMLSQI
                     ARDKGALCWVCRGEFNFYTLADLMKQTPSFTYEGNNPKAEYTLSKMRLLQQEHATTAK
                     NQYRFVGYSMTDGYVEYGDSSLPVRYISDSDMETLRNMQLSLVPKMDIEVAGNPDIKP
                     GMVIEIIVYRYDQENRIDESMPRKLIVKNVAHFEDRVGYTTRMILGVPNK"
     gene            6190..6795
                     /locus_tag="ACIYSB_003760"
     CDS             6190..6795
                     /locus_tag="ACIYSB_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HGC5430713.1"
                     /translation="MRAQVRVLPDWNGVPDDDLPWAEYLLPIGNAFVPTVKGDLVWVE
                     FPYLDVNGRIDTRRPMIVGAAQDAPGGVPNVAPEASGNGSGWTPPEVDGAPPRPQFSA
                     TKDFVIHRNNVLEVRTAGGGYEIANTAAGSRIGMNESGQIYIIGPADVVVNAGGSVNV
                     KSADNISVNGKNITVTADENIDFKAGGTFQAIASNFDFKKG"
     gene            6855..7760
                     /gene="rdgC"
                     /locus_tag="ACIYSB_003761"
     CDS             6855..7760
                     /gene="rdgC"
                     /locus_tag="ACIYSB_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009444180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGC5430714.1"
                     /translation="MRSVAFKNIFIYRLSREVNWSAAEVSDALSKFVFTPCGSQDTAK
                     VGWTPVLGDNLTHEYQGFLLIQHKREEKILPSQVLKEELQKKVLKLEEEQARKLKKTE
                     RDSLKDEVLHSLLPRAFTRKSLAKILIDRSNHLVFVEASSAKKAEDQLALLRKSLGSL
                     PVIPFTPREPLEITMTEWFKSGFPAGFTAGEDATLKGLLDNGGAVRCNKVDLQSDEIM
                     SHIEAGKVATTVAINWMDRASFRVNDDMSIKALTFCDDLYDQNDDIDREDVAQRFDAD
                     FVLFTGELSALFNALVEAIGGEAER"
     gene            7804..8424
                     /locus_tag="ACIYSB_003762"
     CDS             7804..8424
                     /locus_tag="ACIYSB_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430715.1"
                     /translation="MVAPWLEYGVDAAIVDPQHLSTSDERMQSGAVLTRISAIIDSDE
                     VYAFLRKNLQRIVFLAGFPPCTDLAVSGARWFSDKANKDPVFQFKAMQVVWQCYDIAK
                     MIGCPYMIENPVSKISTFWRKPDHIFHPYFFTAYCQEDNYTKKTCLWSGQGFVMPDAL
                     MDESLGKPDNRIHAAPPGPDRANFRSATPHGFAKAVFEANKGVLYE"
     gene            8504..9322
                     /locus_tag="ACIYSB_003763"
                     /pseudo
     CDS             8504..9322
                     /locus_tag="ACIYSB_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(9371..11032)
                     /gene="glnS"
                     /locus_tag="ACIYSB_003764"
     CDS             complement(9371..11032)
                     /gene="glnS"
                     /locus_tag="ACIYSB_003764"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGC5430716.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNIRYSSD
                     YFDQLHAYAVELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFNVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KEAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKLG"
     gene            complement(11301..11588)
                     /locus_tag="ACIYSB_003765"
     CDS             complement(11301..11588)
                     /locus_tag="ACIYSB_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430717.1"
                     /translation="MIKPRKSVKAPEVKDLDLERRIEAFASKADLVPGEQPEDNKVLD
                     KDAPRDFKSIRVGFNEYEYQVLDALSKKHNRSKLNMIRHAILMLAESEEAK"
     gene            complement(11581..12222)
                     /locus_tag="ACIYSB_003766"
     CDS             complement(11581..12222)
                     /locus_tag="ACIYSB_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000585023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGC5430718.1"
                     /translation="MIILVASQKGGCGKSTTSVNICAELARANKDVVLLDADKQGTSA
                     RWASDRNAAEVSPVIHCVQKFGNIRETLLDLDKRYEFVVVDTAGRDSKEMRTGITAAD
                     VLLVPFRPSQPDLDTLAHFVEVFEEALDLMPNPSIKACAVLTMAPSNPVVNEANEAKE
                     YLAEYPQLKLLKTIIRDRKVYRDCMAEGKGVVEMDNGKAKGEIQMLVKELLSD"
     gene            complement(12510..12848)
                     /locus_tag="ACIYSB_003767"
     CDS             complement(12510..12848)
                     /locus_tag="ACIYSB_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGC5430719.1"
                     /translation="MSQERKKVMIYLRPEAYANEKAASEKIKKHSDMARTALLAGLAL
                     GEVDSRLPGLLASLLTEDNNPELIRKMLASFLELPAAVEERPASIEPVKEQVVAKSAS
                     ARNLADSLPD"
     gene            complement(12862..13818)
                     /gene="parM"
                     /locus_tag="ACIYSB_003768"
     CDS             complement(12862..13818)
                     /gene="parM"
                     /locus_tag="ACIYSB_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HGC5430720.1"
                     /translation="MRIFIDDGSTNIKMLWEHDGETRTHISPNSFKRGWSATFGAGKP
                     FNYVIDDEKYSYDLISPDVLPTNNVEWQYSPLNVLAVHHALLTSGIEPQEVEIVVTLP
                     LAEFYDDDAQYNLDNIERKKASLMRPVTLNKGNVFTIKKVTVRPESIPAGIGLCDNLN
                     PAHSVLIVDLGGTTLDVSMVAGQMTAVSRVFGDSNLGVSLVTREVRQALARANTETSN
                     YNVDQLIINRHDEDYLNDNINDPSAVGDVKKAIAASIDRLRTRVLDVIGDFKGYTHVM
                     VIGGGAPLVADAIREQVNIRDDRFFVADDPQLALVHGLKAIG"
     gene            14085..14369
                     /locus_tag="ACIYSB_003769"
     CDS             14085..14369
                     /locus_tag="ACIYSB_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HGC5430721.1"
                     /translation="MSAIATSDTLCSGHGGFPPRLVAETVPWFTVNGKPVVVDGAMFP
                     SHTDGNSAHPGAAVSTRVWFSIGGKGVVCVGDPLSCGSVIANGEDIFQVA"
     gene            14369..15175
                     /locus_tag="ACIYSB_003770"
     CDS             14369..15175
                     /locus_tag="ACIYSB_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430722.1"
                     /translation="MLDSIGEVKAFKILSDAGISTPESITISRAASVKASSLASAIQG
                     IVHADKTYPDSVSAWTTQLLGFSEQLNEASKASSSLADSLSPYTKPSELLQMKIGWEC
                     YAKGNELTPIPAFALVEGMGNVSIPQSLTDALTALKLDALKTAMNAINAKIEAAGSAG
                     GGESNGGQGGAGGAQAPVITQDEIDALREAVTAAEVLLSEINSASEGVVALTGRIKTS
                     TTQATKGLENAVAITLTGSLLDDAVMSPAISLIMPQGVIDALQKNTKKEP"
     gene            15246..15704
                     /locus_tag="ACIYSB_003771"
     CDS             15246..15704
                     /locus_tag="ACIYSB_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430723.1"
                     /translation="MVLKKIILIVIALLAISGIYRTTTAVIEHIRYVRSLEDSKSQLE
                     STNNTLSTALQLSENARSAMLTENERIKALEKEYQRKTAELNANLIKQREESQNEITR
                     LENALRRAGINDVRVPDDVIRMQRERAKAINQRASENYRRSHQQAAGKSD"
     gene            complement(15858..16316)
                     /locus_tag="ACIYSB_003772"
     CDS             complement(15858..16316)
                     /locus_tag="ACIYSB_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430724.1"
                     /translation="MSHANPSKTQYRLMLAIASAIPTSLNPPAGYPAVVDDCFQYYGE
                     DILGQSKALKQLCKAGILHCIGDPDDFVVMLADRDSFLLSWKAGAREARLGNGIGYID
                     YSDCPLAFAGGYMHWHERNRGRQRQYRLSDFNVCHGFEEADSQDIWLQEP"
     gene            complement(16334..16768)
                     /locus_tag="ACIYSB_003773"
     CDS             complement(16334..16768)
                     /locus_tag="ACIYSB_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430725.1"
                     /translation="MWFNFNFLTQIITVMAYRIFVSYKNGAKSHSLNTTSRFLVEAQL
                     ASILAESEILSLAERIVIQFSGRDILNVPALTPSSEVMESIKWPVCGCPARVEEPVTA
                     TLYMPKAVRDWLAVIGNGKVSAGLRKLIEMADIPELKNAWRQ"
     gene            16885..18582
                     /locus_tag="ACIYSB_003774"
     CDS             16885..18582
                     /locus_tag="ACIYSB_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HGC5430726.1"
                     /translation="MNKNIQPARDREREIMNRAASVMAMTVDPTTDAAGNMIADQAAM
                     MENLDKAIQKEAMFEGVNPEVARQIAGGWAMSLHEYKRQHGHYPASDILANAHMALER
                     LMTECASDTHVGTGKAMFESVAQSMRSSDGVMKVAQYAALILPASLGAATSDACTFVP
                     CDRDESNIYELVNVAGTKFGTFEQGDELNMQSAGVYSQMKRLYTLSTKGDGSTKTFQF
                     DIQNFEGQSCPLRAGYNKLLINRKPSKVDDGDGNLYFNDKDCKGNAFSATAKVAYDTG
                     VIDITFTQAPAEGTEIAVQVEINIERNPSLIPVINQAMRKYEVRPSQYVIASEHTVMS
                     ASDLSREHGLELAALQFSAMRNWISHETDIMRLRTLVFHTVYGREFDVALPEAQNYES
                     WVGLLRHAVNALSQEMANRTLTTGIRGGFAGGDAANFLRSLPPQHFQIAPGYVQSPYV
                     QYIGTLFGTIRIYEVPQPVCEQFKAQGYDLGLDDIYFYGRGEGIGKAGLIAGDAVPAI
                     PYVHETNPSLVNRTTLWGSAINEVHPRNGENYFARLRLTRAKEGAIDMLTGKVNEKK"
     gene            18729..19007
                     /locus_tag="ACIYSB_003775"
     CDS             18729..19007
                     /locus_tag="ACIYSB_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HGC5430727.1"
                     /translation="MANIQVTITPSDATDKSFTVESDHPEIVRVDGTTCTALKAGQAV
                     LTIKTNDGNKTAQCTVTVREAPKSVSAVSVEPTTKEVTVGDNFTVSES"
     gene            19075..>19336
                     /locus_tag="ACIYSB_003776"
     CDS             19075..>19336
                     /locus_tag="ACIYSB_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5430728.1"
                     /translation="MNKISFSVGQAAGVAVMSVNADATLTNTSGGASVFAGLVISRRG
                     APGKVLKVDDTTYQSVLGSPIHPRQGAAFEPYRHVERAVKGGS"
BASE COUNT         5350 a         4663 c         4602 g         4721 t
ORIGIN      
        1 atgaagtttt atgttataaa aacaattaac tacatcaaca tgatttaggc caaatttcgg
       61 tcaaaaaata aaatccacaa aaagtggttg acactatttt ggaaatcaca aactgcacat
      121 aatccatcgc gctaacggct cccgttgaag gttcttttga cgattaactt tcagccgaag
      181 cgcggtaggg agtcataacg ccaaagcagg ccgccatgtg cggccttttt ttgtatccgt
      241 catccgtgga ggaaggacaa tggaaaagat cgcaatattc agcctgacca ccagcaagcc
      301 tcaaatgctc actgcaatac tgaaagacgg tgctctcatt attaacgaag taaaacccct
      361 tcccgcgtca gcattggagc aaaagcaaaa aattcctcca gctatagcag ccctgcggaa
      421 aagcaaattt aaggtactgg tagacgaaat tacgccaacg atctccgctg gcaccggagc
      481 aagccaggtg actctcaaga cccgtcatgc cgatggcaga gcagcaatca tcgtcgggat
      541 ggaaagatac agagagttaa aactccagaa gctattatcc ctgccgcaaa ataacaaagg
      601 tgctttcgaa atccccgact ctatcgttga taccgaatac aacggtaacg gagaagaagt
      661 ctacagggtg aactggcagg atatcaggcc ggagcatatt ctgatgatcc tgtgttgcta
      721 cgcgaccgta taccacaacg ttgccagtgc agattacgta gagcagatga ccggcacagt
      781 cgagaaagag caaaaaacag gcatactcgc ttcgttcctg tccattattg ggcatgaaaa
      841 agttgaagca ggcacctccc agccaaagtc actggctggg aaagaagttg atgaagatac
      901 cgtgatactt tgatcacatt agtagctttg ttaactccct caataccagg tagaagagta
      961 acaaaaatat tgacgccagt atttgagtgg acaatgaagc gttcataaac agatcaatta
     1021 gcattaataa ttcgaagata acatccatgt cattcactcc gtttaatttt taatctaatg
     1081 ccagcaaatg aagctggccc ccgcataaag attaatgaaa gtcacttgtc accagtgagg
     1141 ggatttatga accacatccc cctgaactgg tggcctgtct gtttctcgca tttcacaccc
     1201 tgcatacagt ttccaccagg ttatgactga gaggctttgt tatgggctat agcagactcg
     1261 acgataggta cattgaagac gatatttttc gtgcgctgtt tcaccaggaa atgattaagc
     1321 gggtatcgga gtatcactct gataatttcc agtacacgat aaagattgat gaagtatatc
     1381 gttcagacct tgcagcctac agggcgtatg gcaatgcaga tttgcgctgg gtattccggg
     1441 tgctggtggg ccatgagtca gaaatggaag aaatgcccgc cgggaccacg ttaactcttc
     1501 ctgatgtcgc atggctgagg aacaagatcc gtgattacgc gagcgcggaa ccggagatag
     1561 aaaatgcctg atttcctgaa aaaccaggac gggcgctata tcactgatgg cctgtcctct
     1621 aaggacttca cgcgtttatt cgaccttatc agtaaagaac aaacccgtaa gcgccgacaa
     1681 gctcaccgga cgctgacgcc cggcagactg aggaacaaat ccgccgaaga tattctcaag
     1741 ttggggaaga agaaaggcgg cacgttcttt acgcgagacg atctgaaagg cttcgaaaag
     1801 ctacggagta aaacgcgcga aaaatatgac agtaagacgg caggcatcac atacgcccag
     1861 ctggtggcat ccagccaggc tatcgatatt aagcgtgcaa ataacgccgt ggatgacgga
     1921 tctggtatca aaagagctac acccgtatct cttcgccaca atgtgattaa tatccgcgta
     1981 gaagcatcgg atatatccgt ccaccagcac catatcgtcc gtatacgctt tgaagaatgg
     2041 gatcagatgg tcgatgacat cgcagaagac gataaatcag ctctgaaaat cactaaatca
     2101 ctgtgcgccg ggcgggtgtc tttcgattgt gactgtggtc gtcatcaata ctggtatcgt
     2161 tacatcgcta ctgcgggtaa ctttgccttg gccccgccaa aagaatacgc ctatccaaaa
     2221 gttcgtaacc ctaagctaca aggcgtcgcc tgtaaacacg tgatccactc aatgacgcgg
     2281 ttacagtccg ccagttggca aatgagtatt gctcgtgcgt tacaaaaggc cgcaacgcaa
     2341 attgcatttg gtgacgatcg ccgccgtaca actaaacact tctcaaaaga agacgagaag
     2401 gagtttaatc gcaatcgtag cagtaaaacg aacgttgaag ccgccaaacg cgaatggagg
     2461 ctctatcaga aacgccaggc cgctttaagc acaaaactgg caaaggacag cgggaagatt
     2521 gacaagctac gtgaccagct gaccagggcc agaaagttgt ccgatgcaca gaaaaaacgg
     2581 gcagcagcaa aagaagcagc cttgcaacgt gagaaacaga aaaacaagga gcttcagcaa
     2641 cgccttgccg atcaattcgc actgaagaag caagcgttca ttgatgcact ggtcatggca
     2701 ggaacgtcac ctgagcaagc tgaaaaaatg tttatggagt acgtgaagaa aggttcttga
     2761 ttgacaacaa aggcggtcat acagaccgcc aaactcatac accaaccagg ctttcaagag
     2821 gaatgccaaa ctgggaatgt aaacgacgaa tcataggtaa cgttaaattt cgtgtcccat
     2881 ttagcacttc gtaaacacga tttgacttac caatcgccgg ttccagatcc ttcacggtca
     2941 gcccttgctg ttccatacgg aatttaatgg cttcaatagg tgatggtggc tcaattgggt
     3001 aatgcttttt ctcgtactct tcaatgagca ggcacattac ttcaaagaaa tcgccttccg
     3061 gtgtgttcat ttccggttcg ttatcgaaca tcggttcgac cgcacgcagt gcggcctcat
     3121 aatcttgttc tgtacggata ggtttaatgt tcatgattta ctccagttct attgtatccg
     3181 cttcaatggc atcgtattcc ttatgtgttc caataaactt aacgaacatc catcctcgct
     3241 gataagcaat tgcgacaatt aacctgtaat catttccctt aatattgaac accacgcgcc
     3301 tgtttttaag gatgctggct gtccggtact gttctttgat ctcagccgga tttttccagt
     3361 cagcctttgt tgcttcatca acccatgcct ttaatggctg ttctgcatcg ggattcttcg
     3421 cccagaaatc ccttagtgtt tttaccgaaa ttattttcat cacagattcc ttctcgctga
     3481 aagtatagta gtcccaatat gggacaatgt aaagcacttt agtcccatgc tgggatatga
     3541 attgaaatca caatctccag acaatgcacc ctcgatacct gataaatcga cctttgagga
     3601 tgcttatggg ccgttttgac gaatggtttg ctgacgatgc aaacctggca agtaaggagt
     3661 cagaacatga cagcacgcag agagaaacgc cagcgacggt tgaagcggat gcaggaggcg
     3721 cgccggaaca tggcaatacc ggttcggatt cattcagcat tctggaagag caacccgccg
     3781 ccaaagctga tagccatgat aattttgctg atagcaacgg tggccttact ccggatgctg
     3841 gcgaatcaga tatagccata cttccatctt ccctggctgg cagagaaccg actccacagt
     3901 taaaagcacg ctataacggg cataaagcct ttaacgacca aatccgcgca gactggatgc
     3961 tgattattga atccagccca gacgcgtttc aggctctctt atatcgacca gatgttggca
     4021 catatggggt agtcagcgac gaaacaggag aagagtcatt caccgaactg gataacaacc
     4081 aacgcgaact gacttaccag gaacctgaaa tcgtttatgt gctggataac cctgacgggc
     4141 gtgactcttt ccatgcgatt gacgcagacg gtgagcagga tggcttaacc gatgatgttc
     4201 tgattctgcg tattgcagca aataacgtcc ccgtcggctc aattcttgaa tggaatgaag
     4261 agatggtaaa cggcgtagcg cgccgctggt ggtacgtgca ccgtattttt agctacggca
     4321 cacagcatgt tggttcgctt tactactgca tacctgccag gaattttgat acgactcaaa
     4381 acggagtgat cgtatgactt caaataaaac cctcctggcg cgaacaggtg aatggcaaac
     4441 ctcacgcaca ggaaaattcc agaccacggg ctttgaaaac gtggataacg cgtttgcgac
     4501 gctaatcagc aacatttttt ctgatatctt actggtggcc cccgcgccgg aagaaaaacg
     4561 ctttgcgtca ttcctgaatc gtccaccagc agagcgtgtc tatgtggccc gtttcgacaa
     4621 tgcgatcgag tttcttaaag cagttcgtcg cgcaaatgcc gggcaaggga gaaaacctga
     4681 aaaccagaac attaaccggg atgctctccc cctggtcaat atctcacgca atatggatat
     4741 caattatatc aacgatgatc agcagattga ccgcaaaaaa gtagccagtt tttgcgaacc
     4801 ggataccgga atgcctttag cagaactgga atacacccag gccattctga cgtatgacgt
     4861 tacgttaatg gcaactgata aagcgaccat gagtctgatg tgtaattcgc tgggcgcacg
     4921 gcttcgcctg atgacaggta cacaatttga agcaaccact caccttgttc gtgtcccggt
     4981 cccgctgatt tgctcgatac aggatgccaa agaagtcggc tttacggatg tttcagcacc
     5041 aattggagaa gagcgtattt atgccacaca agcgccgata agtgtgattg cagacgtgat
     5101 cacagcatgg gagttggacg caaaacgtat tattaccgaa acctcgatat ctatggggtg
     5161 ataagtggcc caggagttac aacaatattt tctacagtca gtgctcatta acgataacaa
     5221 agtgccacga gactggattt ttaccgcagt atatgtagaa aaaaccagcc tcaaagcccc
     5281 tttgctaaaa ctggaaattc atgacgctac cggcaccgta attgatgact ggaaagccaa
     5341 atacggtgca tcgctggtgg ctgaaatggg cgatccaaac ggtaatgcag gcacttttaa
     5401 aacagatttc tttgttacgt ctgcaatgct ggctggtgat gttgttaccg ttattgctgt
     5461 cagtgaagac gtgcgcaggt ttaagatccc ctccccgcgc acaaatttac ataccaacaa
     5521 aactccagac gctatattca aggcatattc cggcaagctt aaaattacca gcagcgtgct
     5581 aaaacgcgca gttacatatc atctgaatgc cggtgacaaa ccgtcaaaaa tgctctcgca
     5641 gatagcgcga gacaaaggtg cattatgttg ggtatgccgt ggggaattta acttttacac
     5701 cctggctgat ctgatgaagc aaacgccatc atttacctac gaggggaata accctaaagc
     5761 agaatacact ttgtccaaaa tgcgcctact ccaacaggaa catgcgacaa cagcaaagaa
     5821 tcaatatcgt tttgttgggt attccatgac cgatggctac gtcgaatatg gcgatagctc
     5881 actcccagtg cgttatatat ccgactctga tatggaaact cttcgcaata tgcagctgtc
     5941 tctcgtcccc aaaatggata tagaagtcgc aggcaatccg gatataaaac cgggaatggt
     6001 aatagagatt atcgtatacc gctatgacca ggaaaaccgc attgatgaat caatgccccg
     6061 caagctgata gtaaaaaatg ttgcgcactt tgaagaccgc gtaggctaca caacacgaat
     6121 gatattagga gtgccgaaca aatgaagcgt agagcgcaaa ttgttggaac tgtgcacccg
     6181 gcagggctta tgcgtgctca ggtccgtgtt ttaccagact ggaatggcgt tcctgatgac
     6241 gatctaccct gggcagaata cctgttaccc ataggtaacg cgtttgtacc tacagtaaaa
     6301 ggcgacctgg tatgggtaga gtttccttat ctggatgtta atggtcgaat tgataccaga
     6361 cgcccaatga tagttggcgc agctcaggat gcgcctggcg gagtaccaaa cgtcgcgccg
     6421 gaggcgtcag gcaatggtag cggctggacg ccaccagaag tagacggagc gcctccccgc
     6481 ccgcaatttt cggcgacaaa agatttcgtg attcaccgca acaatgtgct cgaagtgcga
     6541 acggctggcg gtggatatga aatagcgaat acggcagcag gctcaaggat tggaatgaat
     6601 gaatcagggc agatatacat cattggtccg gctgatgtag tagtgaatgc gggagggagt
     6661 gttaacgtca aatcagccga taacatcagc gttaatggca aaaacattac tgtgacagcc
     6721 gacgaaaata ttgatttcaa ggctggaggc accttccagg ctatcgcaag caactttgat
     6781 ttcaagaagg gatagaaaac acatccgatt atgttagata attgaataga attttctaac
     6841 acaattggtg aaacatgaga tcagtcgctt tcaaaaacat cttcatctat cgtctttcac
     6901 gtgaagttaa ctggagtgcc gcagaagtat ccgatgctct tagcaaattc gtgtttactc
     6961 cttgcggttc tcaggacaca gctaaagtcg gttggacacc tgttcttggt gacaacctca
     7021 cccatgaata ccagggtttt ctgctaatac agcataagcg agaagaaaaa attctcccct
     7081 cgcaggtgct caaggaagag ttgcagaaaa aagtcctgaa actcgaagaa gagcaagccc
     7141 gcaagctgaa gaaaacggaa agagacagtc ttaaagatga agtcctgcat tcacttttgc
     7201 ctcgcgcgtt cacgagaaaa tcactcgcca aaatcctgat cgaccgcagc aaccatctgg
     7261 tattcgttga ggcaagcagc gccaaaaagg cagaggacca gctggcttta ttgcgaaagt
     7321 cgctcggcag cctgcctgtc attccattca caccacgcga accgctggaa attacaatga
     7381 ccgagtggtt taaaagcggc ttccctgccg gatttacagc aggtgaagac gccacattaa
     7441 agggtctgct cgataatggt ggtgccgttc gctgcaacaa agttgatcta cagtccgacg
     7501 agattatgtc tcacattgag gcggggaaag tagctacgac cgttgcaatt aactggatgg
     7561 accgtgcgtc gttcagagtc aacgatgata tgagtatcaa agccctgaca ttctgtgatg
     7621 atctctatga ccagaacgac gatatcgacc gggaggatgt agcacaacga tttgatgctg
     7681 atttcgtgct ctttacgggc gaattatctg cactgtttaa cgcgctggtg gaggccattg
     7741 gcggtgaagc agaacgataa taaccgaccg cttgttgtga gtctttgtga ttttacgggg
     7801 gcaatggttg ccccctggct ggaatacggt gtcgatgccg ctatcgtcga tccacagcac
     7861 ttatcgacca gtgacgagcg gatgcaatca ggtgccgttc tgacgcgtat tagtgcgatc
     7921 atcgatagcg atgaagtata cgcttttctc cgtaagaatt tgcagcgcat cgtgtttctg
     7981 gccgggttcc caccgtgtac tgacctggct gtttccggcg cgcgctggtt ttcggacaag
     8041 gctaataaag atccggtttt ccagttcaaa gcaatgcagg ttgtctggca gtgttatgac
     8101 attgcaaaga tgatcggttg tccgtacatg attgaaaatc cggtcagcaa aatatcgaca
     8161 ttctggcgta agccagatca catctttcac ccttacttct tcactgcata ttgtcaggaa
     8221 gataactaca ccaaaaaaac atgcctctgg agcggccagg gctttgtcat gcctgatgcc
     8281 ctaatggacg aatcattagg caagccagat aacagaatcc atgccgcgcc accagggcct
     8341 gacagggcta attttcgaag tgctactcca catggattcg caaaggcagt tttcgaagcg
     8401 aataaggggg tgctctatga gtaaatctat agcaagcata gccatagaaa aacaggatac
     8461 gatggctgaa attagctata tgcgcaccat tcggactccg gatgaatatg agcgtccgat
     8521 attcaaatgg gtaggtggta aattctcaga attgcctaca gtgcttgagc atctaccaca
     8581 cggtaagcgg ttaatagaac cattcgttgg tggcggttcc gtatttacga atgcaggatt
     8641 tcgccacaac ctgcttaatg atattaacgg tgacctgatt aacttctatc agacattgca
     8701 gcgagaagga cattcgctcg tcacgctgtc atatagtttt ttcaaaaatt acaacaacgc
     8761 tgacgcttac ctcgaagtgc gtgaggcatt taacagagga aaatatgacc agctacatca
     8821 tgctgccgca tttttgtacc ttaatcgtca ctgttttaat ggcgtaacgc ggtacaacca
     8881 aaatggcgag ttcaacgtgg ggtatggcaa atacaaagcg ccctacttcc cacatgcaga
     8941 gatggaggca tttttggctg atgacgtact caaaaacact tcctttgtat caggagactt
     9001 tgctagcgtc atcgaggcgg ctggtgaaga cgatgtgatt ttttgtgatc cgccgtatga
     9061 accgcttccg gatacagagg gatttaccag ctattcagga aatagcttcc gtttcgatga
     9121 acaaaaacgg cttgtatcac tgttggtgga tgcccaccag cgcggcgcta aggtagtgat
     9181 aacaaatagt ggtgctccaa acatccgtga gctatatgaa ggaaacgggt ttaaagtaca
     9241 tcatatggca gccagacggt cggtttcctg caaggcgtca acacgtgtag ttgctaacga
     9301 tataattgca ataatgaagt aaaaaacgcc gcagtagcgg cgtttacttt ttcatgtggc
     9361 gaaataccta ttaacctaac ttcgcccagg tatcacgcag cccaacggtg cggttaaata
     9421 ccggtttttc cgccgtagaa tggcggctgt cgaggcagaa ataaccttca cgctcaaact
     9481 ggaatgcttt acccgctacc gcttctttca gcgacggttc agcaaagcct tgtttgatca
     9541 ccagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg ttcggcacgt
     9601 tgaacagacg atcgtacagg cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
     9661 gaataacgcc tttcacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
     9721 aagtacagaa gatggtggtg atattacctt cggcatcttt ctccacgcgt tcagccttaa
     9781 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
     9841 tagcttcttc tcggaaatcg gcgcggtcga tccaaatctc accgctaaac ggcacctgac
     9901 ggctacccat ttccggtttg tttggatggt tcggcatggt gaccatttcg ccttcgccct
     9961 ggtagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcatttt
    10021 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
    10081 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc cgcagtgtaa ccacgacgac
    10141 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    10201 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    10261 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac
    10321 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagc gcatcgctga
    10381 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    10441 cagcaaactt aatgcggtac agcaccggat cgcgcatcac gataaacggc gacgccatgt
    10501 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    10561 acagcgccag gttctcttca acgctgcgat cgcggtacgg gctgttttta cccggctgcg
    10621 tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcgtcaaca tacgccaggc
    10681 ctttgttgat cagttcaaca gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    10741 ggatgttgcc agaccagtga aaacctaacc attcaacgtc gtttttaatc gactcgacgt
    10801 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    10861 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atgaccaata tgcagatagc
    10921 catttggttc cggcgggaaa cgggtatgta ctgtggtgtg cttaccactg gccagatctt
    10981 catcgatgat ctgacgaatg aagttagtcg ggcgggcttc tgcctcactc attgtggatt
    11041 cctcaaagcg taaacaacgt ataacagcgt atgatcttat aagccgaata ggctgacaac
    11101 cactagttga ttagaaattg ctttctatcg ttatttcaat aatgagcttt ttcatgaagc
    11161 ccacttttgc gcataagtaa attaatgaca aatggtgcac aattggtacc aaaaaggatg
    11221 taaaagtgca ccaaattagt ggtgtattgg tgcacctttg gttcaaaact ttttaagttc
    11281 taaaaccaac ctgaaagact ttattttgct tcctcagact ccgctaacat gaggatagca
    11341 tggcggatca tattcaattt gctgcgatta tgctttttgc ttaacgcatc aagtacctgg
    11401 tactcgtatt cattgaagcc aacacgaatg gatttaaaat cacgtggggc gtccttatcg
    11461 agcactttgt tgtcttctgg ttgctcaccc ggcaccaaat cagctttgct ggcaaaagcc
    11521 tcaattcggc gttcaagatc gaggtctttt acttcggggg cttttaccga tttacgaggc
    11581 ttaatcactt aataactcct taaccaacat ctggatttca cctttagctt tcccgttgtc
    11641 catctcaaca acgcccttcc cttcagccat acaatcgcgg taaaccttgc gatcgcgaat
    11701 gatggttttc agcaacttca gttgcggata ttcagccagg tactctttgg cctcattggc
    11761 ttcattcaca accggattgg atggggccat tgttaagact gcgcacgcct taatgctagg
    11821 attaggcatc agatccaaag cctcttcaaa gacttcaaca aaatgcgcca gagtgtctaa
    11881 atctggctga gatggcctga atggaacaag caaaacatcc gccgcagtaa taccggtgcg
    11941 catttctttg ctatcacgac cagcggtatc aacaaccaca aattcataac gcttgtccag
    12001 atcaagaagt gtttcacgaa tattcccaaa cttctggacg caatgaatta ctggagaaac
    12061 ctctgccgca ttacggtccg atgcccaacg ggcagatgtt ccttgcttat ctgcatcaag
    12121 tagaactacg tccttatttg cgcgggcaag ctccgcacaa atatttacgc tggtggttga
    12181 tttgccacag ccaccttttt ggctggctac caatataatc atatccgttc ctcggtgtga
    12241 ttttggtatc aaaacggttc cagataagag cctttttgat gcgggacagg ctctataatg
    12301 gagtcatgaa tgtttggcaa agtataacat caatcagacc aaatacacct tatattgcat
    12361 cctatttaac acgaaatcag ccccaaattg gaaccagata caaaattgca ccataacagc
    12421 acctaactgg ttccagatta acaccaatta gacaccatta atgcataaaa aaggctgcaa
    12481 tttgcagcct taaaccagca catcttctgt caatcaggta gagagtcagc cagattgcgc
    12541 gccgacgcgc ttttggcaac aacttgctcc ttcactggct caatggaggc agggcgctcc
    12601 tcaaccgcag ctggtagttc caggaaggat gccagcattt ttcggatcag ctccgggtta
    12661 ttgtcttcgg tcagcaggga agccagtaat cctggaagcc tgctatcgac ttctcccagc
    12721 gcaagccctg ccaacaatgc ggttcttgcc atatcgctat gtttttttat cttctcgcta
    12781 gccgcctttt cattggcata agcctctggg cgaagataaa tcatcacttt cttacgttcc
    12841 tgagacatgg acattacctc gttaaccgat tgctttcagg ccatgaacaa gagcaagttg
    12901 cgggtcatcc gccacgaaga aacggtcatc acgaatatta acttgctcgc gaattgcatc
    12961 tgccaccagc ggtgcgccac caccgatcac catgacatgc gtatatcctt taaagtcgcc
    13021 aatcacatca agaacgcggg tacgcagacg gtcaatgctt gcggcaatgg cctttttcac
    13081 atcaccaacc gctgatgggt cattgatatt gtcgttcaga taatcttcat cgtggcggtt
    13141 aataatgagc tgatcgacat tgtaatttga cgtttcggta ttggctctgg caagtgcttg
    13201 ccttacttcc ctggtaacca gagagacgcc aagattcgaa tcgccaaaaa cacgggaaac
    13261 tgccgtcatt tgcccggcga ccattgaaac atcaagggta gttccaccca aatcgacgat
    13321 aagaacagaa tgggcagggt tcagattgtc gcacaggcca attcctgccg gaatagactc
    13381 cgggcgtacc gtaactttct taatcgtgaa cacattgcct ttattcagcg tgacggggcg
    13441 cataaggctg gctttcttgc gctcgatgtt atcgagattg tattgcgcgt cgtcgtcgta
    13501 aaactccgcc agaggcagcg tgaccacaat ttctacttcc tgcggctcaa tgccacttgt
    13561 cagcagggca tggtgaacag ccaggacgtt aagcgggctg tattgccatt ccacgttatt
    13621 cgtcggcaga acatctggcg agatcaaatc atacgaatac ttttcgtcgt caatgacata
    13681 gttaaacggc ttgcccgcac caaatgttgc tgaccatccg cgcttaaagc tgttagggct
    13741 gatgtgagtg cgagtttccc cgtcgtgctc ccacagcatt ttgatattgg ttgaaccatc
    13801 atcgataaaa atgcgcatct tcttgctcca cttctgagac acatttgaga atcaaaatgc
    13861 aattttaaaa gagaagcaac caaaaatcca caccaaaaga gataaaaaag agtgattatt
    13921 gagttcattt tgagaatatc ttaatcacca aatgaatgat ggtctgacag tgagatttat
    13981 ttgagaatga attgagaata caaaaatatt ccgaaattta actctcagtt tgaatgacga
    14041 aataacaaag tgatgacaat aggaggataa ccaggagatg aagaatgagc gcaatagcca
    14101 ccagcgacac cctttgttct ggtcacggag gatttccgcc gcgactggta gctgaaaccg
    14161 ttccctggtt caccgttaac ggtaagccag ttgttgtaga tggtgccatg tttcccagtc
    14221 acactgacgg taacagtgca catccagggg cagctgtatc gacgcgagta tggttttcga
    14281 taggtggaaa gggagttgtc tgtgtaggcg atccgctttc ttgtggttct gttatcgcca
    14341 acggcgagga tatttttcag gttgcataat gcttgattca ataggtgaag ttaaagcctt
    14401 caaaattctg tcagatgccg gtatctctac accagaatct ataaccataa gccgggcagc
    14461 ttctgtaaaa gcctcatcgc tcgccagcgc catacaggga atagttcacg ccgataaaac
    14521 ctatcctgat tctgtttcgg cctggactac tcaattgctt ggcttttctg aacagctcaa
    14581 tgaggccagc aaagcgtctt catcactggc tgattctctt tccccataca ccaagccatc
    14641 agagctgtta cagatgaaga ttggctggga atgctacgca aaaggtaacg agctgacccc
    14701 catacctgct ttcgcccttg ttgagggaat ggggaatgtt tccattccgc aatctctaac
    14761 ggacgcattg acggcgctaa aacttgatgc tctgaaaacc gccatgaacg ccattaacgc
    14821 aaaaattgaa gctgccggaa gtgctggtgg tggtgaaagc aatggtggac agggtggtgc
    14881 tgggggcgct caggctccag tcattactca ggatgaaatt gatgctttac gggaagcagt
    14941 gacagccgcc gaagttttac tctccgaaat taactcagca agtgagggtg tcgtagccct
    15001 tacaggtcgc attaaaacgt caaccactca ggcaaccaaa ggtttagaga acgctgtcgc
    15061 catcacgtta actggttctc tactggatga tgcagtaatg agtccggcaa tcagtttaat
    15121 catgccacaa ggcgttatcg atgcgctcca aaagaacacc aaaaaggaac catagaaaca
    15181 ccacttaggc acacaaaagg atgtaaattg gtacatgttt aacaccaaaa atgaacctgg
    15241 agaggatggt tttgaaaaag ataattttga ttgtaattgc gcttttagcc atttctggaa
    15301 tttaccgaac aacgacggca gttatcgaac acatcaggta tgtccgatcg cttgaagact
    15361 caaaatcaca actggaaagc acgaacaaca cgctttctac cgcattacag ttaagtgaga
    15421 acgctcgctc agccatgctg accgaaaacg aaagaattaa agcactggaa aaggagtacc
    15481 agcgcaaaac agcagaactg aatgccaacc taatcaaaca acgagaggaa agccagaatg
    15541 aaatcactcg ccttgaaaat gcgttgcgcc gtgctgggat taacgatgtg cgtgtgcctg
    15601 atgacgttat caggatgcag cgggaacggg caaaagccat caaccagcgt gccagtgaaa
    15661 attaccgtag aagccaccag caagccgcag gcaaatctga ttgattactc cccttgttta
    15721 gtgccagagg acacgccagt gctgttaaac aagtatccga catacgctga acagttattc
    15781 agcacgatcg acgaatgcaa ccaacgaaac gcactgaata accaaagaaa cagggagatt
    15841 tgggaaggga agggggatca aggctcctga agccagatgt cctggctgtc agcttcttcg
    15901 aaaccgtgac agacgttaaa gtcactcaag cgatactgac gctgacggcc tctatttcgc
    15961 tcatgccaat gcatatatcc accagcaaat gccagcgggc aatcgctata gtctatgtag
    16021 ccaatgccat tccccaaacg cgcttcgcgc gccccggctt tccaggacag cagaaaagag
    16081 tcacgatctg ccagcatgac aacaaaatca tccggatcgc ctatgcagtg aagaatcccg
    16141 gctttgcaca actgttttag tgctttcgac tgtcccagaa tgtcttcacc gtaatactga
    16201 aaacaatcat caacaacagc gggatagcca gcagggggat tcaggctggt tggtatagct
    16261 gacgcgatcg ccagcattaa gcgatattgt gtttttgatg gattcgcgtg actcattttg
    16321 cccccctgtt tactcattgt cgccatgcgt tttttaactc tggaatatct gccatttcaa
    16381 ttaactttcg cagtccagca ctgactttcc cgttgccaat cacagcaagc cagtctctta
    16441 ctgctttcgg catatagagc gtagcggtta ccggctcttc aactctcgcc gggcatccac
    16501 atacaggcca tttaattgat tccatgactt cggaggacgg ggtgagggcg gggacattaa
    16561 gaatatctct accagaaaac tggatgacga ttcgttcagc gagggagagt atctcacttt
    16621 cagcaagaat tgatgccagt tgcgcctcaa caagaaagcg gctcgttgtg ttcagggagt
    16681 ggcttttcgc gccattttta tacgaaacaa agatccgata cgccataacc gtaatgattt
    16741 gtgttagaaa attaaaatta aaccacataa caacacgcgt gttaacagat tttattttac
    16801 taatgctatt gactaaatca caaagttggt attgttagcg cgtcaaaaga accttcacgc
    16861 cgcgcccgaa tttagggctt aaccgtgaat aaaaacattc agccagctcg tgatcgcgag
    16921 cgcgagatta tgaatcgggc ggcaagcgtc atggctatga ccgtcgatcc gaccaccgat
    16981 gctgcgggca atatgattgc tgatcaggca gcgatgatgg agaacctgga taaagctatc
    17041 cagaaggagg ctatgtttga aggcgtaaat ccagaggttg cacgtcagat cgctggtggg
    17101 tgggctatgt ctctgcatga gtacaagcgc cagcatggtc actacccggc aagcgatatc
    17161 ctggcaaacg cacacatggc acttgaacgc ctgatgactg aatgtgccag cgatacacac
    17221 gtaggcactg gcaaggcaat gtttgagtct gtcgcgcaat caatgcgtag ctctgacggc
    17281 gtaatgaaag ttgctcaata tgctgcacta attctgcctg cctctctggg tgctgctacc
    17341 agtgatgcct gtacttttgt tccttgtgat cgtgatgagt caaatattta tgaactggtc
    17401 aacgtagcag gcacaaaatt cggcaccttc gagcaaggtg acgaactgaa tatgcaatcc
    17461 gctggtgttt attctcaaat gaagcgcctg tatacccttt ccacgaaagg ggacggcagc
    17521 accaaaactt tccagttcga catccaaaat tttgaggggc agtcctgccc gctgcgtgct
    17581 ggttataaca agctgcttat caaccgcaaa ccgtccaagg ttgacgacgg cgatggcaac
    17641 ctttatttca atgataaaga ctgcaaaggc aatgcattct cagctaccgc aaaagtcgct
    17701 tatgacactg gtgtcatcga cattaccttc actcaagcgc cagcagaagg gactgaaatt
    17761 gcggttcagg tcgaaattaa catcgagcgc aatccaagcc tgattccggt aatcaaccag
    17821 gctatgcgta agtatgaagt tcgaccgtct cagtacgtaa ttgcttccga acacaccgta
    17881 atgtccgctt ccgatttaag ccgtgaacac ggccttgaat tggcggcact tcagttctcc
    17941 gcaatgcgga actggatctc ccatgaaacc gacatcatgc gtctgcgcac cctggtattc
    18001 cataccgttt atggtcgtga atttgatgtg gctctgcctg aagctcaaaa ctatgagtcc
    18061 tgggtcggcc tcctgcgcca cgcagtaaac gccctgtctc aggaaatggc aaaccgcacc
    18121 ctgaccacag gtattcgcgg tggcttcgca ggtggtgacg cagctaactt cctgcgttct
    18181 ctgcctccac agcacttcca aatcgcgccg ggctacgttc agtcaccgta tgtgcagtac
    18241 atcggcaccc tgtttggcac gattcgtatt tacgaagtgc cgcagcctgt gtgtgagcaa
    18301 ttcaaggctc agggatatga cttaggtctg gatgacattt atttctatgg tcgcggtgaa
    18361 ggcatcggta aagccgggct gatcgctggt gatgcggtcc cagcaatccc atatgtgcac
    18421 gaaaccaatc catctctcgt taaccgcacc actctctggg gtagtgctat caacgaagtc
    18481 cacccgcgca acggtgagaa ttatttcgcc cgcctgcgtc tgactcgtgc caaagaaggt
    18541 gctatcgaca tgctgacagg caaggttaac gaaaaaaagt gatggtggcg aacgtgtcgg
    18601 tatcaccaac gtcacttgac gtagatgaag gtgaaactat cgccgcgaac acttcaaagg
    18661 cacagaaaaa aacaaagcag aagtaaggca aaaacacgcc cctgattaag gggcgttcag
    18721 gagaaaaaat ggcaaacatt caagtaacta tcacgccatc tgacgcaaca gacaaatctt
    18781 tcactgttga gtcagatcac cctgaaattg tccgggttga tggcactaca tgtaccgctc
    18841 ttaaggccgg gcaggcagtg ctgacaatca aaacaaatga cggcaataaa accgcacagt
    18901 gcacagtcac tgttagggag gcacctaaaa gcgtaagcgc cgtcagtgtt gaaccaacta
    18961 cgaaagaagt aaccgtaggc gacaacttca cggtaagcga atcctgatca gaggctgccc
    19021 attaaccgtg ggtagccttg cctcgttctc tcgctttaga ggataagaac cagcatgaat
    19081 aagattagtt tttcggtagg gcaggcagct ggcgttgctg taatgtcagt aaatgctgac
    19141 gctaccctca ccaataccag cggtggcgca tccgtctttg ctgggcttgt aatatcgcgt
    19201 cgtggcgcac cgggtaaggt actgaaagtt gacgatacga cttatcaaag cgtattaggt
    19261 tcaccgattc accctcgcca gggcgcggca tttgaaccat atcgccacgt ggagcgtgca
    19321 gtaaaaggcg gctctg
//
LOCUS       DBBGLQ010000080        19274 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.080, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000080 DBBGLQ010000000
VERSION     DBBGLQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.080"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            28..831
                     /gene="ydjO"
                     /locus_tag="ACIYSB_003777"
     CDS             28..831
                     /gene="ydjO"
                     /locus_tag="ACIYSB_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HGC5430729.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLQSTMTALTFFISTGQC
                     DCDEIAESNFNYMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEIQI
                     RGINKDFIGMDVTSVFRPEKMTNLISIKNRLVIHYLRTIYYEQNYIHPVGSIFAAIQK
                     NESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQ
                     AHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(835..2226)
                     /gene="tcyP"
                     /locus_tag="ACIYSB_003778"
     CDS             complement(835..2226)
                     /gene="tcyP"
                     /locus_tag="ACIYSB_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGC5430730.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(2359..2949)
                     /gene="ydjM"
                     /locus_tag="ACIYSB_003779"
     CDS             complement(2359..2949)
                     /gene="ydjM"
                     /locus_tag="ACIYSB_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGC5430731.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(3112..3780)
                     /gene="hxpB"
                     /locus_tag="ACIYSB_003780"
     CDS             complement(3112..3780)
                     /gene="hxpB"
                     /locus_tag="ACIYSB_003780"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGC5430732.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLADVKLSSLTELTAK
                     DLLG"
     gene            3927..4463
                     /gene="yniB"
                     /locus_tag="ACIYSB_003781"
     CDS             3927..4463
                     /gene="yniB"
                     /locus_tag="ACIYSB_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGC5430733.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENKE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4504..5364)
                     /gene="yniA"
                     /locus_tag="ACIYSB_003782"
     CDS             complement(4504..5364)
                     /gene="yniA"
                     /locus_tag="ACIYSB_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGC5430734.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5470..5760)
                     /gene="ghoS"
                     /locus_tag="ACIYSB_003783"
     CDS             complement(5470..5760)
                     /gene="ghoS"
                     /locus_tag="ACIYSB_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGC5430735.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5861..6790)
                     /gene="pfkB"
                     /locus_tag="ACIYSB_003784"
     CDS             complement(5861..6790)
                     /gene="pfkB"
                     /locus_tag="ACIYSB_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGC5430736.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            7077..7835
                     /gene="ydiY"
                     /locus_tag="ACIYSB_003785"
     CDS             7077..7835
                     /gene="ydiY"
                     /locus_tag="ACIYSB_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGC5430737.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGHTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7888..7995)
                     /gene="yniD"
                     /locus_tag="ACIYSB_003786"
     CDS             complement(7888..7995)
                     /gene="yniD"
                     /locus_tag="ACIYSB_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGC5430738.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(8167..10066)
                     /gene="espL1"
                     /locus_tag="ACIYSB_003787"
                     /pseudo
     CDS             complement(8167..10066)
                     /gene="espL1"
                     /locus_tag="ACIYSB_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10590..12518
                     /gene="thrS"
                     /locus_tag="ACIYSB_003788"
     CDS             10590..12518
                     /gene="thrS"
                     /locus_tag="ACIYSB_003788"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGC5430739.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12522..13064
                     /gene="infC"
                     /locus_tag="ACIYSB_003789"
     CDS             12522..13064
                     /gene="infC"
                     /locus_tag="ACIYSB_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGC5430740.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            13161..13358
                     /gene="rpmI"
                     /locus_tag="ACIYSB_003790"
     CDS             13161..13358
                     /gene="rpmI"
                     /locus_tag="ACIYSB_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGC5430741.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            13411..13767
                     /gene="rplT"
                     /locus_tag="ACIYSB_003791"
     CDS             13411..13767
                     /gene="rplT"
                     /locus_tag="ACIYSB_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGC5430742.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13884..14008
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13890..13934
                     /gene="pheM"
                     /locus_tag="ACIYSB_003792"
     CDS             13890..13934
                     /gene="pheM"
                     /locus_tag="ACIYSB_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGC5430743.1"
                     /translation="MNAAIFRFFFYFST"
     gene            14218..15201
                     /gene="pheS"
                     /locus_tag="ACIYSB_003793"
     CDS             14218..15201
                     /gene="pheS"
                     /locus_tag="ACIYSB_003793"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGC5430744.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            15216..17603
                     /gene="pheT"
                     /locus_tag="ACIYSB_003794"
     CDS             15216..17603
                     /gene="pheT"
                     /locus_tag="ACIYSB_003794"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGC5430745.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17608..17907
                     /gene="ihfA"
                     /locus_tag="ACIYSB_003795"
     CDS             17608..17907
                     /gene="ihfA"
                     /locus_tag="ACIYSB_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGC5430746.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            18139..18318
                     /locus_tag="ACIYSB_003796"
     CDS             18139..18318
                     /locus_tag="ACIYSB_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGC5430747.1"
                     /translation="MDSKSAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKD
                     IPLPTLKSIKKQAGL"
     gene            18341..18739
                     /locus_tag="ACIYSB_003797"
     CDS             18341..18739
                     /locus_tag="ACIYSB_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGC5430748.1"
                     /translation="MLYAIAIDRGDSSFGVRVPDLPGCFSGGDSFQDAVDSAREAIEA
                     HVELLVESGESIPVATNLDEWQSDPEYSDAVWALVEVDVSRLMGKSEKINVTLPSLLI
                     RRIDQFVESHPEYGSRSGFLARVAADRVLG"
BASE COUNT         5104 a         4417 c         4797 g         4956 t
ORIGIN      
        1 aattttgtta acaatatgag gcgggagatg ttttctacaa acttaataca gagtaattat
       61 ggtgatttaa atattaaaag cctggctttc gactctttta aagaaaggct gcagtctact
      121 atgacagcac tcactttctt tatatcaacg ggtcaatgtg actgcgatga aatagcagaa
      181 tcaaacttta attatatgat tgcctatatg tctaacatta actatgatgc ctcaaagcca
      241 ggagcgccag cacttagttt tgatacatat ctgcaggaca atgttaaata tagagtaata
      301 attaataatc tttatggtag tgaaattcag ataagaggta taaataaaga ttttattgga
      361 atggatgtca cttctgtatt tcgcccagaa aaaatgacca atttgatcag tataaaaaac
      421 aggttggtta ttcactattt aaggactata tactatgaac aaaattatat acatcctgtc
      481 gggagcattt ttgctgcgat tcaaaaaaat gagagtctcc taaagtttcc ttcgataata
      541 tcaatgctta atatcaatct tctttttaat ccactaaatc ttccgggaat gggaagtggt
      601 gtgcttgaag atattatgag tattccagat agttcactta gaaaacgtct cgggtatgag
      661 gttttaagct tctcgctaca ggcacattca ttgagccagg aatgtattga taagttggac
      721 atttttttcg ctgacgatct gtttaaatat gagtcagtat gcatcgccgc gatggaacat
      781 ttaaaaagta aggcaacggc ccctatacaa aacggaccgt tgccagcata agaattaatg
      841 gtgtgccagt tcggcgtcgt cttcgctatc cagaatggct ttatcggttt gcttcagcca
      901 ctggctggtc agcgtgccag ctgtcatcga gccactaacg tttaacgccg tacggcccat
      961 gtcgataagc ggttcaacgg agattaacag cgccaccagg gttactggca ggcccatcgc
     1021 aggcagtaca atcagtgcgg cgaaagttgc accaccaccg acaccggcaa cgcctgcgga
     1081 actaacggta acaataccga ccagcgtcgc aatccacatc gggtccagcg ggttaatgcc
     1141 aaccgtaggc gcaaccatca ccgccagcat tgccggatac aaaccggcgc agccgttctg
     1201 accaatggtt gcaccgaaag aggcggcgaa actggcgatg gattcaggaa cgcccagacg
     1261 acgcgtttgt gcttccacat tcagtgggat agacgcagca ctggaacggc tggtaaaggc
     1321 aaacgtcagc acaggccata ccttacggaa gtacttcagc ggactcacgc cattaatgcc
     1381 cagcagaatg ccatgcactg caaacataat cagcagaccg aggtaggacg cgacaacgaa
     1441 acttcccagt ttgatgatgt cttgcaagtt agaacctgca accactttgg tcattagagc
     1501 cagaacgccg taaggggtca actgcatgac caggcgaacc agtttcatca cccagctttg
     1561 tagggtatcg atagcggcta agacgcgttc acctttcggc gcatcatcct tcagcagttt
     1621 caatgcagct acgccgagga atgcggcaaa aattaccacg ctgataattg acgtcggatt
     1681 ggctccggta agatcggcaa acgggttttt cgggataaag gacaagacca gctgcggaac
     1741 gctcagatca gagactttac caacatagtt actttcaatg gcgttcagac gtgcagtttc
     1801 tgcaccaccc tgaaccagac cttcagccgt caaaccaaac aggttggtga ccagcacacc
     1861 gaccagcgcc gcaatcagcg tggtaaacaa aagcgtaccg atggtcagaa aactgatttt
     1921 gcctaactga gatgcgttat gcagacgggc aaccgcgctc agaatagagg cgaagactaa
     1981 cggcataacg atcatttgca gcagttgaac atagccgtta ccaacgatgt taaaccactg
     2041 tacagaatct ttaagtacct ggctgtcaga accataaatg gtatgcaggg caaggccaaa
     2101 aaccacaccc atcaccagac ccaccagcac ttttttcgcc agactccact gtttatggcg
     2161 ggtctgagcc agcgcaaaca gcagtacaac gaacaccacg atgttcgcaa ttaatggaaa
     2221 gttcatcccc gttctcctga tcttattatt tctatcactg cgtagcagtc atagtgttgg
     2281 cgcaagatta acagaactgt gagctggctc ttatattcaa atggaattgc ttattccaga
     2341 aaggtgattt ttgccttttt agtattcaac ctgatgctta ataagccgat gaaactggat
     2401 ctgcaaggta ttgatcattt gcgatgacca acgaacagcg ctgttctcgg gtaatgaatg
     2461 gggcatccat accgaccaga caaataatgc catgcagata aaacgttcca gttggttgcc
     2521 cttttgggga accaggatag gcaagcggaa acgccagcgg catggccaga gcaggggaac
     2581 accggcgggt gtcagcatat cggcaagtat gtggctcaaa taacccagca ccattccttg
     2641 tagcgcatca gccggaatga accagccttc cggaacctta aggtaaaagg ttgccagcag
     2701 cgcaaatacc gccagcagac tgtgggtaaa accacgatgc ccaaaagcgc gggcgatcgg
     2761 ttttgatatc cattttaatc gctgcccaag aaacgacttt gggtgatcga tgtccggtaa
     2821 caaacacgtc aggattgcgg aagggacaat atgccaccag tcaccctgtg ccagcacggg
     2881 cgtcagctcg gcatttttgg caaataccgc acaagcaata gaaaagagaa ggtgaccttc
     2941 cgccgtcatg ataaaactca ctaaactgtc gatacgtaca gtataggatt tttatacagt
     3001 gcacggaagt ggtgacggtg taactctttc tcttaaattg acgattaatc cgtcagttga
     3061 atgcaatttc agataaagaa ccaggggcag cgagatcact gcccctggtc attaaccgag
     3121 aaggtctttt gcggtgagtt ctgtcagcga tgagagtttg acgtctgcta atacaaaacg
     3181 tggatcattt tgcgcttctg gcgcaggaac gacgatggaa cgcatgcggg ctgctttaga
     3241 ggcgatcatg ccatttaccg aatcttccag cgctacgcag gtcagagggt caacgcccag
     3301 ttttgctgcg cagtcgagat atacttgcgg atgcggcttg ctgtaaggca gtttttcggc
     3361 cgaggcgagg gcatcgaaac tgtcgcgtaa gtcaaacatg gtcaacactt tttccagcat
     3421 atgtagtggt gacgcggagg ccagtcccac caataaacct tgttctttgc ataacgcaac
     3481 ggcttcgcgc acgcctggta ataatggacg tgtctcttca accagtgaaa tggcacgggc
     3541 aataacccgt tctactactt cctgacggct tggcccattc cacggttgcc gggcgtacca
     3601 aagatcgacc accatatcga tgcgtaaacc taaggtgtcc ggcagctcgt tacgacggga
     3661 gatatccacc cccaggcttg ccatcacatc cagttcggct cgatcccata aaggttctga
     3721 gtcgataagt aatccatcca tatcaaaaat tgcagcaaga atctgacgcg gggttgacat
     3781 tacaacctct cctttagagc attatgtgta aggtattaag ccgtgcttat gattaagcgc
     3841 ctatcatatc gcctttaaag aatgggcgaa aacagatatc tacaggtaaa ctaagtgaaa
     3901 cgacgcgtct taatcaagga gaactcatga cgtatcaaca agctggacgc attgctgttt
     3961 tgaaaaggat tttgggttgg gtgattttta tacctgcatt gatctctaca ttgatttctt
     4021 tgctgaaatt tatgaatacc cggcaggaaa acaaggaagg cattaatgcg gtcatgctcg
     4081 actttactca tgtcatgatc gatatgatgc aggcgaatac gccttttttg aatctattct
     4141 ggtataactc cccgacgccc aattttaatg gtggcgtgaa cgttatgttc tgggtgattt
     4201 tcatcctgat ttttgtcgga ctggcgttgc aggattccgg tgcccggatg agtcgccagg
     4261 cgcgcttctt gcgggaaggt gttgaagatc aactcattct ggaaaaagcc aaaggggaag
     4321 aggggctaac gcgtgaacaa attgagtccc gtattgttgt tccgcaccat actattttcc
     4381 tgcagttttt ctcactgtat atcctgccgg ttatttgtat tgctgcaggt tatgtgttct
     4441 tttctctgct tgggtttatt taatcaaaag gccgcacgga gcggccatta tcctcaaccc
     4501 atatcatgct gctaataatc tatccaatga ctgctgagca ataaccaaat gctgaccgcc
     4561 aaataacctt gcacgattta acagcgtgta gagttggtaa accggttgac gttcaaggaa
     4621 atcggcaggt agcggggata ctgactgata gccgtcatag atttgtggcg gctgttcagt
     4681 atgcagcggt aacatcgcca ggtcgcactc tcggtcaccc cagtagcagg ccgggtcgaa
     4741 aatgtacggg ccatccggac ccagtgcaca gttgccggac cataaatcgc cgtgcaacag
     4801 agaaggctgc ggttgatgtg aggccagacg ctgctgaata tgctcgacga tggcgtcgat
     4861 attgccgaaa gcgatccctt tttctgcagc cagttccaac tgccagccaa tccgttgttc
     4921 agcaaaaaac gttgaccagc gacgttgcca ggtgttgggc tgtggagttg tggagagcgc
     4981 gttatcgaaa tcaaggccaa attgtggttg gtcgctccat tgatgtaaac gcgcaatttg
     5041 ctgaccaaga ataaatgcgc tatgcgcatc cagcggacga ggtgggagat aatccatcac
     5101 cagaaaactg tagtcacggt cagcgccaac tgcccaaacc ttaggcacgg tgacggtttt
     5161 actacgcgac agtaactcca gttggtcggc ttcggcggta aaaccgggaa gcagttccct
     5221 ttcatcacat ttgacgaaaa aatcatgtcc tgcatagcgc aaatgccatg cggcatggac
     5281 ttctccgcca ggcagttcat tacgcagttc gatttcgcct tcacctaact gctcgctcaa
     5341 aagacgactg attgcctgcc acatgatgtc tctcccatgt tgtctgccag atcataaggt
     5401 tagcgcaaga atgcggtgta aaaacacgag ctaacgcaca ccacagactg attattgccc
     5461 aaaaaccatt tattttctgt tgctattcca ttcctcccag gtcgtgatgg tgatttcagg
     5521 atctttgccg gttgcacttt gggtaagccc cgaaaccagt tctctaattt cttcttcact
     5581 ttgggtactg ataagtccaa aagtgttagt tcccagctca tggatatttc cctcatcatc
     5641 cgtcatagtg agcaaaaaac cgtcgcgagt caggtaatta ttcaactcgt taatttcagt
     5701 caatgtatcc tcatgcaaca tgacggttat gacataacgg acaagatcgc cactggccat
     5761 agttcacccc tttgttatca catgactttt gagcatagtc ggagaaacgc gttgccgaca
     5821 ggttggtgat gattccccca atgctggggg aatgtttttg ttagcgggaa aggtaagcgt
     5881 aaattttttg cgtatcgtca tgggagcaca gacgtgttcc ctgattgagt gtggctgcac
     5941 tccccgcagc tacgccaaaa cgaaccatct cttcaagaga ggcattttct gccagtttca
     6001 gtgtcatcgc gccgaccatg ctgtcaccag cgccaacggt actctggctt ttcaccggtg
     6061 gtggcaccac ctgaatacag ttttcactat caacacccag cgctccttgt ggacccaggg
     6121 aaacgacaac ccgtttggcc ttgccgctat taacgatttc ctgcgcggct ttgcggacat
     6181 cgtccggctg ggtgagttcg cgattcacca gcgcactgag ttctttttgg ttaggcttaa
     6241 ccaactcgat gttaccaatt gccagtgctg cacttaacgc ttcgccagaa ctgtcgacga
     6301 tgcagcggat cccttgtttt tgcgcagcgg aaatcagttg ggttaatttt tccagcttca
     6361 cacctggcgg caggcttccg cttatgacca ggatggcccc ggattcaatt tccagaactt
     6421 gctcttcaag ctggcgaaac tcatcttcat ttaatgccgc gcctggcata acaaaacgat
     6481 actgctcacc gcttgcttcc acatgtacgt gtaaattctg ccgggtccag tctttggctt
     6541 ctacagtagc gacggggaca ttttcatccg ccaacagtga aaccaggtgt tcgccggtcg
     6601 cgccacccgc cgggaagatc gctgtggcac tgcctccaag atgggcaatg gcgcgggcga
     6661 cgttgatgcc gccgcccccg ggttcgaaca ccggtgcggt acagcgcagt tttccttcgg
     6721 gataaatttg cggggtaatt gttgcgctat cgagagaggg cgcaagtgtc aacgtataga
     6781 tacgtaccat catttcctcc tataggctga tttcagtctg gcaccgaatc agcggaaagt
     6841 gaagaaatta acaatatgat ttattggagc tttatttaaa attttgcaga taaatatata
     6901 taaataaaaa tctgttttcg ccacatgctt attcaaaaaa tgaaataaga atacattgct
     6961 aagttattcg cgccttttta taattcgtta cctttccagg gacgctaatc tttgatcctt
     7021 aagaaagttt ccgggaccgc gatggtctct ttttttgttg tacggactct tgataaatga
     7081 agcttttgaa gacagttcct gccatcgtta tgttggcggg gggcatgttt gcgtcactga
     7141 atgcagctgc cgatgattcc gtttttactg tcatggatga tcctgccagc gctaaaaaac
     7201 ctttcgaagg taatctgaac gcaggatatc ttgcacaatc aggcaatacg aaaagctcct
     7261 cacttacggc tgacaccacc atgacctggt atggacacac cactgcctgg tcgctgtggg
     7321 ggaacgccag taatacttct tctaatgatg agcgttcttc agaaaaatat gcggcgggcg
     7381 ggcgtagccg ttttaattta actgattatg actatttatt tggtcaggca agctggctga
     7441 cagaccgtta taacggctat cgagagcgcg atgtgttgac cgcgggttat ggtcgtcagt
     7501 ttttgaatgg cccggtacac agtttccgct ttgaattcgg tccaggtgtg cgttacgaca
     7561 aatataccga caatgccagt gaaacccagc cgctgggcta tgcctccggg gcttatgcat
     7621 ggcagttgac tgacaacgct aaatttacgc agggtgtttc ggtctttggc gcggaagaca
     7681 caacgctgaa ctccgaaagc gcattaaatg ttgctattaa tgaacatttt ggactgaagg
     7741 tggcttacaa cgtcacatgg aactctgaac ctccagagtc tgcaccggaa cataccgacc
     7801 gtcggacaac cttatcgctg ggctacagca tgtaaaatta tctgccggac cgtgttatgc
     7861 ggtctggctg tcttcgtttg tcactgctca gccccaaatc attgctgccc aacgtagcaa
     7921 caacatagcg ccacagattg ccagtagcac caccaccatc ttccagtgtt ttttatcaaa
     7981 gcgtttagtc ggcataccag tgtcctgcat atctcttgtg cgtgataacg gagtgtaaca
     8041 aaagggacag gcatcaaggg attttttgtt attacagccg tcgggtggcg ttgtgcacat
     8101 ggtcactgtt tctgcgtttt atgaaaaaaa taaaattatg tatcgatata tatcgtaaat
     8161 agctggttat aaatacgtct ttatttcatc agcactaaaa ctcaggcttt ggctgattgc
     8221 attaatagca gcataatatg tttctacagt tttataatgc ttatgatgaa tggctatatg
     8281 aacagctgac atgttgtcat gatttttagc ggccaatagt gtaaataact gatgttgact
     8341 aaaagaatgt ttttttgcaa aagcacccag cgtcgatata taagataaca cgatgtcttc
     8401 attacccttg ctcatggcga tgtataatgc aggggttacc tgtgctgtag ctccttttaa
     8461 taaatccatg atgttagctt tgctcaactt gtatttaaag gcgatgccat taattttaga
     8521 gaggaaccgt gtgacacaca aagggtgatt attttccatt gcggcgtata atcctggctc
     8581 accatcatgt attttcatgg ctaaaatact catcaccagt tttttgttta aaagctcttg
     8641 tgctattgga gtggttaatg cattcagaaa gatatcgatt atttcatcga atccatacaa
     8701 catggagata taaagtccag gtgtttcgtt agcactttta gtttgcagta atttaaccag
     8761 attatcttca tgaatgatgt gattatctac cagtgactga atttcttgca taaatatttt
     8821 cacgttgtca gtatgtccgt tcatcatagc caaaaataaa gcaggtgtac caacgctatt
     8881 ctgcgcagtt attatttcaa tgatttcttt ttcagggagt atgggcaact gtttgaataa
     8941 tgtaataagc tgttgagtct ggcctgtagc cattattaac aacattgccg atggatgtat
     9001 aggggcaaat ggcattcgtg atgcaatacc cgcgaaaata gcccctggaa ctcccacagg
     9061 taataggggc agaatagcaa ttgcacagtt gcgaacatat ctatcagccc aggtctgagc
     9121 atcgggacct gaactgatga acttatcaag cgaaagaaag ccccgtgctg tcctgtgggt
     9181 gtctctgacg gcaacgttgg tatcgttcgg gtcgtaaacg gacacaacat aatttaattg
     9241 tccatacact gactgcttta ttcgtagcct tactgacaaa gtatgattgt ctatatataa
     9301 aatcgcggac atatgactga cattgtttgc catcattcga caaaagaaaa tatctaaagc
     9361 gaagccaaaa tccttactcg cgataacgcg actccctgca ggaggatggc gaagtaatgc
     9421 tctgaactcc gcgtcccgga ctaaggggac atgctgctgt atggattcaa cggaggagta
     9481 atgtttaatc aggttgactt tgccttttcc atttctcaat gaatcaaggg cgtattgcaa
     9541 tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc aaaaaacatg cactaccatt
     9601 gagatttact gttgggtgta tagttttttt ctctatggat ttttttagac agtttcatac
     9661 aatggttacg atcttcagca gttaactgtt cctgttcaat ataatccagt atgcttcgca
     9721 aatggttagc tttaataggc gtggtttcca gtaataagga aatgatttct tttttatagt
     9781 gtaatgtaat gagtatattt tctgtatgga tgggtgaagt ggcaaagtta atggtaaaaa
     9841 gggcagtgga ataacaattt tgtgatattt ttcgacagtt tgcggtaaat tcctgagtaa
     9901 cacagtatga cagcatcgaa cttaagtcag aggtatcaag tagggtgatg agctggcgcg
     9961 caaattgatt aataggctct gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat
    10021 gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc tgttcgcctt
    10081 atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac gaaatgtgca
    10141 cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca cttcgtcatt
    10201 aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg ctggattcag
    10261 tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt
    10321 tgttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc gaatcaatgt
    10381 gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgctcaact gggtggaagg
    10441 tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc atttgttggc
    10501 tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg atctttcgtg
    10561 tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct gatggcagcc
    10621 aacgccatta cgatcacgct gtaagcccca tggatgttgc gctggacatt ggtccaggtc
    10681 tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct tgcgatctga
    10741 ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt ctggagatca
    10801 ttcgtcactc ctgtgcgcac ctgttagggc acgcgattaa acaactttgg ccgcatacca
    10861 aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat cttgaccgca
    10921 cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt gctgagaaaa
    10981 actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc
    11041 gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat gacaagccag
    11101 gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc
    11161 gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca
    11221 acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa gcacttaacg
    11281 cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc
    11341 tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac aacgacggct
    11401 ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag taccagtatc
    11461 aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg
    11521 acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac tgcattaagc
    11581 cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc
    11641 tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca ggttcgctgc
    11701 atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc tgtactgaag
    11761 aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg tacagcactt
    11821 ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg
    11881 acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa aacaacatcc
    11941 cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt
    12001 atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc tctttgccgt
    12061 ctcgtctgag cgcttcttat gtaggcgaag acaatgaacg taaagtaccg gtaatgattc
    12121 accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa gagttcgctg
    12181 gtttcttccc gacctggctt gcgccggttc aggttgttat catgaatatt accgattcac
    12241 agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag
    12301 cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg cgtcgcgtcc
    12361 catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc gttcgcaccc
    12421 gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag ctgcaacaag
    12481 agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc ggaaaacgag
    12541 ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa
    12601 caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg gagaaagcag
    12661 aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg gtttgtcgta
    12721 taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa
    12781 agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat gaaggcgact
    12841 atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa gccaaaatca
    12901 cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa gtgcttaatc
    12961 gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg aagatcgaag
    13021 gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt caagtagcac
    13081 tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt tattaacaat
    13141 gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct
    13201 tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt cacattctga
    13261 ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg
    13321 atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt tttaactttt
    13381 taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg tgttattgca
    13441 cgtgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc gcgttctcgc
    13501 gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta ccgtgaccgt
    13561 cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt
    13621 cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc tgttgaaatc
    13681 gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac cgctctggtt
    13741 gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt ctccctcttt
    13801 tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa taaaggtctt
    13861 ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc ttcttttttt
    13921 actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg
    13981 aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa agcctgtctc
    14041 atcaggtgta attgtcgcag ccagactgga cacggccagc gcggagaaac cggagcgtat
    14101 ttaagtacgt gagaatttcg agcacagccc gggaccaaaa tggcaagtaa aatagcctga
    14161 tgggataggc tctaagtcca acgaaccagt gtcaccactg acacaatgag gaaaaccatg
    14221 tcacatctcg cagaactggt tgccagtgcg aaggcggcca ttagccaggc gtcagatgtt
    14281 gccgcgttag ataatgtgcg cgtcgaatat ttgggtaaaa aagggcactt aacccttcag
    14341 atgacgaccc tgcgtgagct gccgccagaa gagcgtccgg cagctggtgc ggttatcaac
    14401 gaagcgaaag agcaggttca gcaggcgctg aatgcgcgta aagcggaact ggaaagcgct
    14461 gcactgaatg cgcgtctggc ggcggaaacg attgatgtct ctctgccagg tcgtcgcatt
    14521 gaaaacggcg gtctgcatcc ggttacccgt accatcgacc gtatcgaaag tttcttcggt
    14581 gagcttggct ttaccgtggc aaccgggccg gaaatcgaag acgattatca taacttcgat
    14641 gctctgaaca ttcctggtca ccacccggcg cgcgctgacc acgacacttt ctggtttgac
    14701 actacccgcc tgctgcgtac ccagacctct ggcgtacaga tccgcaccat gaaagcccag
    14761 cagccaccga ttcgtatcat cgcgcctggc cgtgtttatc gtaacgacta cgaccagact
    14821 cacacgccga tgttccatca gatggaaggt ctgattgttg ataccaacat cagctttacc
    14881 aacctgaaag gcacgctgca cgacttcctg cgtaacttct ttgaggaaga tttgcagatt
    14941 cgcttccgtc cttcctactt cccgtttacc gaaccttctg cagaagtgga cgtcatgggt
    15001 aaaaacggta aatggctgga agtgctgggc tgcgggatgg tgcatccgaa cgtgttgcgt
    15061 aacgttggca tcgacccgga agtttactct ggtttcgcct tcgggatggg gatggagcgt
    15121 ctgactatgt tgcgttacgg cgtcaccgac ctgcgttcat tcttcgaaaa cgatctgcgt
    15181 ttcctcaaac agtttaaata aggcaggaat agattatgaa attcagtgaa ctgtggttac
    15241 gcgaatgggt gaacccggcg attgatagcg atgcgctggc aaatcaaatc actatggcgg
    15301 gcctggaagt tgacggtgta gaaccggttg ccggcagctt ccacggcgtg gtcgttggtg
    15361 aagtggttga gtgtgcgcag catccgaacg ctgacaaact gcgtgtgaca aaagtgaatg
    15421 tcggcggcga tcgcctgctg gacatcgtct gcggtgcgcc aaactgccgt cagggcctgc
    15481 gtgtagcggt agcgaccatt ggtgctgttc tgccgggtga tttcaaaatt aaagcggcga
    15541 aactgcgtgg cgaaccgtct gaagggatgc tgtgctcctt ctctgaactg ggcatttctg
    15601 acgatcacag cggcattatc gaactgcctg cggatgcgcc gattggcacc gatatccgtg
    15661 aatacctgaa acttgatgac aacaccatcg aaatcagcgt gacgccaaac cgtgccgact
    15721 gcttaggcat cattggtgtt gcgcgtgacg ttgccgtgct gaaccagctg ccgctggttc
    15781 aaccggaaat cgttccggtt ggtgcgacca tcgacgacac gctgccgatt acagtcgaag
    15841 cgccggaagc ctgcccgcgt tatcttggcc gtgtggtaaa aggcattaac gttaaagcgc
    15901 caactccgct gtggatgaaa gaaaaactgc gtcgttgcgg gatccgttct atcgatgcag
    15961 ttgttgacgt caccaactat gtgctgctcg aactgggcca gccgatgcac gctttcgata
    16021 aagatcgcat tgaaggcggc attgtggtgc ggatggcgaa agagggcgaa acgctggtgc
    16081 tgctcgacgg tactgaagcg aagctgaatg ctgacactct ggtcatcgcc gaccacaaca
    16141 aggcgctggc gatgggcggc atcttcggtg gcgaacactc tggcgtgaat gacgaaacac
    16201 aaaacgtgct gctggaatgc gcgttcttta gcccgctgtc tatcaccggt cgtgctcgtc
    16261 gtcatggcct gcataccgat gcgtctcacc gttatgagcg tggcgttgat ccggcactgc
    16321 agcacaaagc gatggaacgt gcgacccgtc tgctgatcga catctgcggt ggtgaggctg
    16381 gcccggtaat tgatatcacc aacgaagcaa cgctgccgaa gcgtgcaacc atcactctac
    16441 gtcgtagcaa actggatcgc ctgatcggcc atcatattgc ggatgagcag gtaactgaca
    16501 ttctgcgtcg tctcggctgc gaagtgaccg aaggcaaaga cgaatggcag gcagttgcgc
    16561 cgagctggcg tttcgatatg gagattgaag aagatctggt tgaagaagtc gcgcgtgttt
    16621 acggctacaa caacatcccg gatgagccgg tacaggcaag cctgattatg ggtactcacc
    16681 gtgaagctga cctgtcgctc aagcgcgtga aaacgctgct caacgacaaa ggctatcagg
    16741 aagtgatcac ctacagcttc gttgatccga aagtgcagca gatgatccat ccaggcgttg
    16801 aagccttact gctgccaagc ccgatctctg ttgaaatgtc agcaatgcgt ctttctctgt
    16861 ggactggcct gctggcaacc gtggtgtaca accagaaccg tcagcagaac cgtgtgcgca
    16921 ttttcgaaag cggtctgcgt ttcgtaccag atactcaggc accgttgggc attcgtcagg
    16981 atctgatgtt agccggtgtg atttgcggta accgttacga agagcactgg aacctggcaa
    17041 aagagaccgt tgatttctat gatttgaaag gcgatcttga atccgttctc gacctgaccg
    17101 gtaaactgaa tgaggttgag ttccgtgcag aagcgaatcc ggcactgcat ccggggcaat
    17161 ccgcagcgat ttatctgaaa ggtgaacgta ttggttttgt tggggttgtt catcctgaac
    17221 tggaacgtaa actggatctt aacggtcgca ctctggtgtt cgaactggag tggaacaagc
    17281 tcgcagaccg cgtggtgcct caggcgcgcg agatttctcg cttcccggcg aaccgtcgtg
    17341 acatcgcggt ggtggtcgca gaaaacgttc ccgcagcgga tattttatcc gaatgtaaga
    17401 aagttggcgt aaatcaggta gttggcgtaa acttatttga cgtgtaccgc ggtaagggtg
    17461 ttgcggaggg gtataagagc ctcgccataa gcctgatcct gcaagatacc agccgtacac
    17521 tcgaagaaga ggagattgcc gctaccgtcg ccaaatgtgt agaggcatta aaagagcgat
    17581 tccaggcatc attgagggat tgaacctatg gcgcttacaa aagctgaaat gtcagaatat
    17641 ctgtttgata agcttgggct tagcaagcgg gatgccaaag aactggttga actgtttttc
    17701 gaagagatcc gtcgcgctct ggaaaacggc gaacaggtga aactctctgg ttttggtaac
    17761 ttcgatctgc gtgataagaa tcaacgcccg ggacgtaacc cgaaaacggg cgaggatatt
    17821 cccattacag cacggcgcgt ggtgaccttc agacccgggc agaagttaaa aagccgggtc
    17881 gaaaacgctt cgcccaaaga cgagtaatct gatctaacta aaaaggccgc tctgcggcct
    17941 tttttctttg tataatgcat tgatttataa ataaaaatta attcatgatc cacaaattga
    18001 ccgtggtcag tggttttttc ttgccgcagt tttggggagg cagtcaatta ttctataact
    18061 ctaataactc cgaacccttg ctttgactag ttgttggggt tataataact ccaacaacga
    18121 aagacggagt acggggggat ggatagcaaa agtgcaatag caatgataga ggcggatgga
    18181 tggtatcttg ttagggttaa ggggagtcat catcaattca aacatccgac aaagaagggg
    18241 ctggtaacgg taaaacaccc tcaaaaagat atacctttac caactttaaa aagtattaag
    18301 aaacaggcgg ggctgtaagc cccaccttct ggaggtctca atgttatatg caatcgctat
    18361 tgaccggggg gattcatctt ttggtgttcg cgtcccagat ctgcccggct gtttttctgg
    18421 cggtgatagt tttcaggatg cggtagacag tgcccgtgaa gccattgagg cacacgtaga
    18481 attactggtt gaatctggtg agtctatccc tgtcgctaca aatcttgatg aatggcaatc
    18541 agatcctgaa tacagtgatg cagtgtgggc gctcgttgag gtggatgtgt cccgcctgat
    18601 ggggaaatcg gaaaaaatca atgttaccct gccatctctg cttattcgtc gcattgatca
    18661 gttcgttgag tctcatcctg aatacggtag ccgctctgga tttctggcgc gtgttgctgc
    18721 agacagggtt cttggataaa aaaaagcccg cgcaacggcg ggcaaaagac aagacttttc
    18781 gtttgtatat catggataat ggacacccct tcctgttgtc agggggaagg tacccgtagc
    18841 taatctactc actttgtgcc cgcagctcaa ccctgaagtt ttcatggaaa atcagggcag
    18901 cttcatatgt gccaggaagc tgcggcgatg ataactgcca aaaaaatgcc cgctttccgg
    18961 cgggcaaaac agcaatgtga gttgtctgtc attgatactg gacacccttc ctgttgtcag
    19021 gtggaaggca cccgtggtta atctactgtc tttgtgcttt tggctcaacc ctgtagtttc
    19081 catggaaaat cagggctgct ttgtgtgcgg cagggaaggc gcctcgatgg caggcgcaaa
    19141 aaaaatgccc gtgcttcggc gggcaaaata aggaagtaca ggttacatgt tattgataaa
    19201 gtagcattgc tctgtccgtc tttaatctaa cgagttttga gtttgttcac aatattgtta
    19261 cctgtcatgt tata
//
LOCUS       DBBGLQ010000081        19240 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.081, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000081 DBBGLQ010000000
VERSION     DBBGLQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.081"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..481)
                     /locus_tag="ACIYSB_003798"
     CDS             complement(<1..481)
                     /locus_tag="ACIYSB_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="HGC5430749.1"
                     /translation="MSAGAQAIAAKPRQAPMSGFERYLTLWVALCIVAGVALGQGLPD
                     VFQAIGRMEVAQVNLPVGLLIWVMIVPMLVKIDFGALHQVKEHWRGIGVTLFVNWAVK
                     PFSMALLGWLFIRQVFAPFLPADQLDSYIAGLILLAAAPCTAMVFVWSRLTNGDPLFT
                     "
     gene            complement(478..984)
                     /locus_tag="ACIYSB_003799"
     CDS             complement(478..984)
                     /locus_tag="ACIYSB_003799"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HGC5430750.1"
                     /translation="MTERIYNVLILCTGNSARSIMAEALINTMGQGRFRAYSAGSHPT
                     GKVNPFAVEKVESVNYPTENLRSKSWDEYATPDAPKMDFIITVCDNAAGEMCPVWPGQ
                     PISAHWGFEDPAAVEGTDAEKRRAFEQTFRHMMNRVRLFVNLPLKMLDQTAIKRELAN
                     IGKTEQEA"
     gene            complement(981..1748)
                     /gene="arsH"
                     /locus_tag="ACIYSB_003800"
     CDS             complement(981..1748)
                     /gene="arsH"
                     /locus_tag="ACIYSB_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
                     /protein_id="HGC5430751.1"
                     /translation="MRLRHLSDPDSLPALDKSFAIERPALGLAPDAPPVRILLLYGSL
                     RARSFSRLAVEEAARLLQFFGAETRIFDPSDLPLPDQVQSDDHPAVKELRALSEWSEG
                     QVWCSPERHGQITSVMKAQIDHLPLEMAGIRPTQGRTLAVMQVSGGSQSFNAVNTLRL
                     LGRWMRMFTIPNQSSIAKAFQEFDAAGRMKPSPYYDRIADVMEELVRFTALVRPHREA
                     LTDRYSERKAAGHVIDEATDLSSIAIAPQPLPESETS"
     gene            complement(1745..2077)
                     /locus_tag="ACIYSB_003801"
     CDS             complement(1745..2077)
                     /locus_tag="ACIYSB_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGC5430752.1"
                     /translation="METINAVAALAAIAQESRLAVFRLLVQAGPAGMAAGKISEAAGI
                     PPSSLSFHLKELAHAGMVTSRQEGRFVIYEANFSTATNLVAFLTENCCGGQVCNLSCT
                     TEAGKVLA"
     gene            complement(2359..3123)
                     /locus_tag="ACIYSB_003802"
     CDS             complement(2359..3123)
                     /locus_tag="ACIYSB_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HGC5430753.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(3630..4130)
                     /locus_tag="ACIYSB_003803"
     CDS             complement(3630..4130)
                     /locus_tag="ACIYSB_003803"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGC5430754.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(4258..5097)
                     /gene="sul1"
                     /locus_tag="ACIYSB_003804"
     CDS             complement(4258..5097)
                     /gene="sul1"
                     /locus_tag="ACIYSB_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HGC5430755.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(5091..5438)
                     /locus_tag="ACIYSB_003805"
     CDS             complement(5091..5438)
                     /locus_tag="ACIYSB_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HGC5430756.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(5656..6891)
                     /locus_tag="ACIYSB_003806"
     CDS             complement(5656..6891)
                     /locus_tag="ACIYSB_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc58 family transposase"
                     /protein_id="HGC5430757.1"
                     /translation="MPRKKKTPVVTPAIDLPKEFLEKLIPGPMDAAGVEAVFQQLKKA
                     VIERALGAELGLHLADAEGGSGNHRNGRSGKTVLTDEGPLRIDVPRDRTGTFEPQLIP
                     KHERRFAGFDDRILSMYARGMTVREIQGHLAEMYSVEVSPEFISKVTDEVMAEVTAWQ
                     ARPLEAMYPVVFFDALRVKIREDGVVRNKAVYLALGVLADGTRDILGLWIENTEGAKF
                     WMKVFNDLKTRGVNDILIGVADGLKGFPEALEAVFPATTLQTCIVHLIRNSLDYAGWK
                     DRKALAAAIKPIYTAPTAEAAEAELDAFEAGDWGERFPTVVAAWRRAWTRVVPFFAFP
                     PDVRRVIYTTNAIESVHARLRKIIKTRGHFPSDEAATKLIWLALRNITAEWSRASQHW
                     KAAMNQFAIIYEDRFTLAR"
     gene            complement(7159..8652)
                     /locus_tag="ACIYSB_003807"
     CDS             complement(7159..8652)
                     /locus_tag="ACIYSB_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HGC5430758.1"
                     /translation="MCAPLPAAHAAARYARHLPERTLLYALVQAHYPDFIARLEAEDR
                     PLPEYVREEFETYLRCGVLEHGFLRVVCEQCRAERLVAFSCKKRGFCPSCGARRMAES
                     ARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVHRVIAGWLADQAGVD
                     HASAQCGAVTLIQRFGSALNLNVHFHMLWLDGVYEDTTERPQRKPRLHRTRAPTSAQL
                     TELAGTIAHRVCRHLSRRGWLEGEDESVFLSDSAGSDDGMDGLRMSSMTYRIATGRDA
                     GRKVVTLQTLPGDAGSLEGDAGKVGGFSLHAGVAAEAHESHKLEKLCRYITRPAISEQ
                     RLSISPQGRVRYQLKTPWRNGTTHVEWDAVDFIAKLAALVPPPRAHLTRFHGVFAPNA
                     NLRAQLTPSGRGRRPAGDAVPVDASAHDEPRSPEQKRRAMSWAQRLKRVFSIDVTTCA
                     HCGGAVRIVASIEDPKAIRAILAHFEKHGALEQAHYRPAARAPPPAA"
     gene            complement(8877..10514)
                     /gene="groL"
                     /locus_tag="ACIYSB_003808"
     CDS             complement(8877..10514)
                     /gene="groL"
                     /locus_tag="ACIYSB_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGC5430759.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELADKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAVAAGMNPMDLKRGIDKAVTSAVEELKKISKPCSTSKEIAQVGSISANSDTDIGE
                     LIAKAMDKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNPQSMQAELEDP
                     FILLHDKKISNVRDLLPILEGVAKAGKPLLIVAEDVEGEALATLVVNTIRGIVKVCAV
                     KAPGFGDRRKAMLEDMAILTGGTVISEEVGLSLEKATINDLGRAKKVQVSKENTTIID
                     GAGDTADIEARIKQIKAQIEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRAKAAIAELKGANEDQNHGIAIALRAMEAP
                     LREIVTNAGDEPSVVLNRVAEGTGAFGYNAANGEFGDMIEFGILDPTKVTRSALQNAA
                     SIAGLMITTEAMVAEAPKKEEPAAPGGGMGGMGGMDF"
     gene            complement(10570..10857)
                     /gene="groES"
                     /locus_tag="ACIYSB_003809"
     CDS             complement(10570..10857)
                     /gene="groES"
                     /locus_tag="ACIYSB_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009684394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGC5430760.1"
                     /translation="MNIKPLYDRVVIKRTEEEKVSAGGIVIPDSAAEKPIKGQVVAVG
                     EGKVFDNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIFAVLG"
     gene            complement(11097..11996)
                     /gene="aac(3)-VIa"
                     /locus_tag="ACIYSB_003810"
     CDS             complement(11097..11996)
                     /gene="aac(3)-VIa"
                     /locus_tag="ACIYSB_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HGC5430761.1"
                     /translation="MTDPRKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPH
                     VSLRAVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSW
                     PAFDPDHAPAYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYG
                     PRSPIARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWV
                     TTSDWDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFG
                     IEWLEARHAAPAAAALKPKQRRD"
     gene            complement(12116..12954)
                     /gene="aadA1"
                     /locus_tag="ACIYSB_003811"
                     /pseudo
     CDS             complement(12116..12954)
                     /gene="aadA1"
                     /locus_tag="ACIYSB_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            13100..14113
                     /gene="intI1"
                     /locus_tag="ACIYSB_003812"
     CDS             13100..14113
                     /gene="intI1"
                     /locus_tag="ACIYSB_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HGC5430762.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            14393..15130
                     /locus_tag="ACIYSB_003813"
     CDS             14393..15130
                     /locus_tag="ACIYSB_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGC5430763.1"
                     /translation="MRRTKPVAAPMVARVYLRVSTDAQDLERQEAITTAAKAAGYYVA
                     GIYREKASGARADRPELLRMIGDLQPGEVVIAEKIDRISRLPLPEAERLVASIQAKGA
                     RLAVPGVVDLSDLAAEAQGVAKIVLEAVQIMLFRLALQMARDDYEDRRERQRQGIELA
                     RQAGRYKGRRADPKRRAQVVALRKSGYSINKTAELAGYSAAQVKRIWAEVSQAEAKQH
                     GAFVEDALTEADALAAVGQDERQEERA"
     gene            15127..15351
                     /locus_tag="ACIYSB_003814"
     CDS             15127..15351
                     /locus_tag="ACIYSB_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430764.1"
                     /translation="MKKPNQDDEPFFITEEIAAEMIAGGYEFELPPIPCTIRLRDVLE
                     RMTDAELALQPGEIADQERERCRRKPCSTS"
     gene            15562..17055
                     /locus_tag="ACIYSB_003815"
     CDS             15562..17055
                     /locus_tag="ACIYSB_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HGC5430765.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(17231..17533)
                     /locus_tag="ACIYSB_003816"
     CDS             complement(17231..17533)
                     /locus_tag="ACIYSB_003816"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430766.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(17620..18435)
                     /gene="sul2"
                     /locus_tag="ACIYSB_003817"
     CDS             complement(17620..18435)
                     /gene="sul2"
                     /locus_tag="ACIYSB_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HGC5430767.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(18676..>19240)
                     /locus_tag="ACIYSB_003818"
     CDS             complement(18676..>19240)
                     /locus_tag="ACIYSB_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tat pathway signal sequence protein"
                     /protein_id="HGC5430768.1"
                     /translation="LKRRDAFLKKSALAVSVALLLSAQAQAVLTGPVDANSSSLLIGE
                     NSFITNSTGTANNTFLLGGGAFNMDSPGSLQFGSFSGVYNSPHSVTLGRDAGQAESKY
                     GVAIGKSAEVLNSQQSVAIGGWAGIENSSGSVALGHGSQVSGENNVVSVGAGPEGYGE
                     SVKGAPETHSIVNKTFLSFIFNGLFSC"
BASE COUNT         3648 a         5870 c         5944 g         3778 t
ORIGIN      
        1 gcgtgaaaag cgggtcgccg ttggtaaggc ggctccacac gaacaccatt gccgtgcagg
       61 gcgcggcggc cagcaagatc aagcccgcga tgtagctgtc cagttgatcg gccggcagga
      121 acggagcgaa cacttgtcgg atgaacagcc accccagcag ggccatcgag aatggcttga
      181 cggcccaatt cacgaacagc gtgacgccga tgccgcgcca atgctctttg acctggtgca
      241 gcgccccgaa gtcgatcttg accagcatcg ggacgatcat tacccaaatc agcaggccca
      301 ccggcaaatt gacctgggcc acttccatgc ggcctatggc ctggaacacg tcaggaaggc
      361 cctggccgag cgcaacaccg gcgacgatgc acagggccac ccacagcgtc aggtaacgct
      421 cgaagccgct catcggggcc tggcgcggct tcgcggcgat ggcctgtgcg ccggcgctca
      481 tgcttcctgc tcggtcttgc cgatgttcgc cagctcgcgc ttgatggccg tctggtcaag
      541 cattttgaga ggcagattca cgaacaggcg gacgcggttc atcatgtgcc ggaaggtttg
      601 ttcgaaggcc cggcgcttct cggcgtcagt gccttcgacg gcggccgggt cttcaaatcc
      661 ccaatgcgcc gagatcggct gaccaggcca caccggacac atttcgccgg cggcgttgtc
      721 gcagacggtg atgatgaagt ccatcttcgg tgcgtcgggc gtggcgtact cgtcccagct
      781 cttgctgcgc agattctcgg tcgggtaatt caccgactcc accttttcga cggcgaaggg
      841 attgaccttg ccggttgggt gactgccggc gctgtaggcg cggaagcgcc cttgccccat
      901 cgtgttgatg agagcttcgg ccatgatgct gcgcgccgaa ttgccggtgc agaggatgag
      961 gacgttatag attctttcgg tcatgatgtt tcgctttctg gtagtggctg gggagcaatg
     1021 gcgatggatg aaagatcggt ggcctcgtcg atgacgtggc cggccgcttt ccgttcggaa
     1081 tagcggtctg tcagtgcttc acggtgcggc cgtaccagcg ccgtgaagcg caccagttct
     1141 tccatcacat cggctattcg gtcgtagtac ggcgagggct tcatgcggcc cgccgcgtcg
     1201 aactcttgga acgctttggc gatactcgac tggttcggaa tggtgaacat tcgcatccag
     1261 cggccgagca gacgcaaggt gttcacggcg ttgaagctct gcgagccgcc ggatacctgc
     1321 atcacggcca gggtgcggcc ttgggtcggc cggatgccgg ccatttcaag cggcagatgg
     1381 tcaatctgcg ccttcatgac actggtaatc tgaccgtggc gttccgggct gcaccagact
     1441 tgtccctctg accactcgga cagggcgcgc agctccttga cggccgggtg atcgtcgctt
     1501 tgcacttgat cgggcaacgg caaatcggac gggtcgaaga tgcgtgtttc tgcgccaaag
     1561 aattgcagca gccgggccgc ttcctcgacg gccagccgtg agaacgagcg ggcgcgcagc
     1621 gagccataca gcagcaggat acgcaccggc ggggcgtcgg gtgccagccc gagcgccggg
     1681 cgctcgatgg caaaggattt gtccaaagcg ggcaaagaat cggggtcgga aagatggcga
     1741 agtctcatgc caacaccttc ccggcttccg tggtgcaaga caggttgcac acctggccgc
     1801 cgcagcagtt ttccgtgaga aaggccacca ggttagtggc cgtcgaaaag ttcgcctcgt
     1861 agatcacaaa tcggccttct tgccgcgacg tgaccatgcc ggcgtgtgcc agctctttca
     1921 ggtggaagga tagcgaagac ggcgggatgc cggccgcttc gctgattttt ccggcggcca
     1981 tgccggcggg gccggcctgg acgagaagcc ggaacaccgc gaggcgcgat tcttgagcga
     2041 tggccgccag ggcggctaca gcatttatcg tttccatgtt tcaataatag tcgaaatatg
     2101 gagtgtgctc aatagctcat ttaagctgta aaaatgagct aggcgcattt agtgtcattt
     2161 tcagaagacg actgcaccag ttgattgggc gtaatggctg ttgtgcagcc agctcctgac
     2221 agttcaatat cagaagtgat ctgcaccaat ctcgactatg ctcaatactc gtgtgcacca
     2281 aagcgaggtg agcatggcga cggaggctct gttgcaaaga ttggcggcag tcagaggtag
     2341 gctgtcgctc tgcgccgatc aggcggctgc tgcgaaatgg tggttgagca tgcccatggc
     2401 ctccgtcagc gccgagggcc caatgccaaa agctctctcc acaaggcgca cctcgcccct
     2461 gatgccgggc tgcaggcacc aggggcgagc ctgtcctttg cgcagggctc gcatgacttc
     2521 gaatcccttg atcgtggcat aggccgtggg gatcgatttg aaaccgcgca ccggcttgat
     2581 cagtatcttg agctttccgt gatcggcctc gatcacgtta ttgagatact tcacctgccg
     2641 gtgggccgtc tcccggtcca gctttccttc gcgcttcaat tcggtgatcg ctgcaccata
     2701 gctcggcgct ttgtcggtat tgagcgtggc aggcttttcc cagtgcttca ggcctcgcag
     2761 ggccttgccc aggaaccgct tcgctgcctt ggcgctgcgg gtcggcgaca ggtagaaatc
     2821 gatcgtgtcg ccccgcttgt cgactgcccg gtacaggtag gtccacttgc cccgcacctt
     2881 gacgtaggtt tcatccaggc gccagctcgg atcaaagcca cgccgccaga accagcgcag
     2941 ccgcttctcc atctccgggg cgtagcactg gacccagcga tagatcgtcg tatggtcgac
     3001 cgaaatgccg cgttccgcca gcatttcctc aaggtcgcga tagctgatcg gatagcgaca
     3061 ataccagcgc accgcccaca ggatcacatc accctggaaa tggcgccact tgaaatccgt
     3121 catcgttccg tccgtccaat ctccgccaag catgctcaag cttcacgatt tttgcaacag
     3181 agcccacacg agtattgagc atagtcgaga ttggtgcaga tcacttctga tattgaactg
     3241 tcaggagctg gctgcacaac agccattacg cccaatcaac tggtgcagtc gtcttctgaa
     3301 aatgacattt ggtatctctc ataaacggat gtttttgaga gaactatctt cggccttcac
     3361 acgcacgaaa ggcggcgaag ctccgccgtt aatccgtccg ccggagatct cgcccaggca
     3421 ggctgaaggc cgagcaagcc tgacaggccc gaaaagcccg gcacgggcgt cggcggcgat
     3481 gacggcggcg gcattatcca gggttgatga tggaagtgga ggatatcgac aacctctcgc
     3541 gcaaccaaga catcgcggtc ggactgcaag tgatcttgaa gccacgggcc cgtcccaccc
     3601 cgacatggac ctcgatgccc gaacggacgt tagatttcga gttctaggcg ttctgcgatg
     3661 aaggttggat cccagccggg attgaaagtg tcgacgtggg tgaatccgag ccgctcgtat
     3721 aggccacgca ggttcgggtg gcagtcgagc cgcagcttgg cgcacccctg cgttcgcgcg
     3781 gcatggcggc aagcctcgat cagcgcggag ctgacacccc ggcccgcatg tgtccgtcgc
     3841 accgcgagct tgtgcagata tgcggcctcc cccttgaggg cgtcgggcca gaactcggga
     3901 tcctcggccg acaaggtgca acagccgacg atgccgtcgc tgcaactcgc gactaggagc
     3961 tcggatctca ggacgaaggt ctccgcgaat gtccggtcga tccgcgcgac gtcccaggcg
     4021 ggcgttccct tggcggacat ccacgccgca gcgtcgtgca tcagccgcac aacctcgtcg
     4081 atatcacccg agcaggcgac ccgaacgttc ggaggctcct cgctgtccat tcgctcccct
     4141 ggcgcggtat gaaccgccgc ctcatagtgc agtttgatcc tgacgagccc agcatgtctg
     4201 cgcccacctt cgcggaacct gaccagggtc cgctagcggg cggccggaag gtgaatgcta
     4261 ggcatgatct aaccctcggt ctctggcgtc gcgactgcga aatttcgcga gggtttccga
     4321 gaaggtgatt gcgcttcgca gatctccagg cgcgtgggtg cggacgtagt cagcgccatt
     4381 gccgatcgcg tgaagttccg ccgcaaggct cgctggaccc agatccttta caggaaggcc
     4441 aacggtggcg cccaagaagg atttccgcga caccgagacc aatagcggaa gccccaacgc
     4501 cgacttcagc ttttgaaggt tcgacagcac gtgcagcgat gtttccggtg cggggctcaa
     4561 gaaaaatccc atccccggat cgaggatgag ccggtcggca gcgaccccgc tccgtcgcaa
     4621 ggcggaaacc cgcgcctcga agaaccgcac aatctcgtcg agcgcgtctt cgggtcgaag
     4681 gtgaccggtg cgggtggcga tgccatcccg ctgcgctgag tgcataacca ccagcctgca
     4741 gtccgcctca gcaatatcgg gatagagcgc agggtcagga aatccttgga tatcgttcag
     4801 gtagcccacg ccgcgcttga gcgcatagcg ctgggtttcc ggttggaagc tgtcgattga
     4861 aacacggtgc atctgatcgg acagggcgtc taagagcggc gcaatacgtc tgatctcatc
     4921 ggccggcgat acaggcctcg cgtccggatg gctggcggcc ggtccgacat ccacgacgtc
     4981 tgatccgact cgcagcattt cgatcgccgc ggtgacagcg ccggcggggt ctagccgccg
     5041 gctctcatcg aagaaggagt cctcggtgag attcagaatg ccgaacaccg tcaccatggc
     5101 gtcggcctcc gcagcgactt ccacgatggg gatcgggcga gcaaaaaggc agcaattatg
     5161 agccccatac ctacaaagcc ccacgcatca agcttttgcc catgaagcaa ccaggcaatg
     5221 gctgtaatta tgacgacgcc gagtcccgac cagactgcat aagcaacacc gacagggatg
     5281 gatttcagaa ccagagaaag aaaataaaat gcgatgccat aaccgattat gacaacggcg
     5341 gaaggggcaa gcttagtaaa gccctcgcta gattttaatg cggatgttgc gattacttcg
     5401 ccaactattg cgataacaag aaaaagccag cctttcatga tatatctccc aatttgtgta
     5461 gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag tttggctgtg
     5521 agcaattatg tgcttagtgc atctaacttt gtgggtatag gaagttcaac cgcccttttt
     5581 tgcgagtctg gcaggtagga agcgggggcg tgtccgattt tttgtgtgtg aggcggttta
     5641 gtccggaagt ctctttcagc gagccagcgt gaatcggtct tcgtagatta tcgcgaactg
     5701 gttcatcgcc gccttccagt gctgagacgc gcggctccac tcggcggtga tgttccgcag
     5761 cgccagccag atcagcttgg tggcggcctc gtcgctgggg aagtggccgc gggtcttgat
     5821 gatcttgcgc aggcgcgcat gcacgctctc gattgcgttg gtcgtgtaga tcacccggcg
     5881 cacgtccggc gggaacgcga agaacggcac cacgcgcgtc caggcacggc gccaggcggc
     5941 gaccaccgtc gggaagcgct cgccccagtc gccggcctcg aacgcatcca gctcggcctc
     6001 tgcggcctcg gcggtcgggg ccgtgtagat cggcttgatc gccgccgcca gcgccttgcg
     6061 gtccttccag cccgcatagt ccaggctgtt gcggatcagg tgcacgatgc aggtctgcag
     6121 cgtggtcgcc gggaacaccg cttccagcgc ctccgggaag cccttcaggc cgtcagccac
     6181 gccgatcagg atgtcgttga cgccgcgcgt cttgaggtcg ttgaacacct tcatccagaa
     6241 cttcgcgccc tcggtgttct cgatccacag cccgaggatg tcgcgggtgc cgtcggccag
     6301 cacgcccagc gccagataca ccgccttgtt gcgcaccacg ccgtcctcgc ggatcttcac
     6361 ccgcagcgcg tcgaagaaca ccaccggata catcgcttcc agcggccgcg cctgccatgc
     6421 ggtgacctcg gccatcacct cgtcggtgac cttgctgatg aactccggcg acacctcgac
     6481 gctgtacatc tcggccaggt gcccctggat ctcacgcacc gtcatgccgc gggcgtacat
     6541 cgagaggatc cgatcgtcga acccggcgaa gcgacgctcg tgcttgggga tcagctgcgg
     6601 ctcgaacgta cccgtccggt cgcgcggcac gtcgatgcgc agcggaccct cgtcggtcag
     6661 caccgtcttg ccgctgcggc cgttgcggtg gttgccgctc ccgccctcgg cgtcggccag
     6721 gtgcaggccg agctccgcgc ccagcgcccg ctcgatcacg gccttcttca gctgctggaa
     6781 cacggcttcg accccggccg cgtccatcgg acccgggatc agcttctcga gaaattcctt
     6841 cggcagatca atggccggcg tgaccaccgg cgtcttcttc ttgcgtggca tacatgctcc
     6901 ttggcagcat catggtatgc ctctcacaca aaatttctga caccctcgga agcggccgcg
     6961 caagcgccgc tttgggcaca gatcgggcac agatcggggg ttcatgcgtg ggttttcgac
     7021 atcgcggggc cacgcagcgg ctcagtgcgt agccatttct caccgaaacc cggcacaggg
     7081 ccgagcgcag ccctgcgggg tcgtggccgc atcggatcgg actctggctt ggctgccggc
     7141 tgtgtggccg gcacctcgtc acgcggccgg cggcggcgcg cgcgctgcgg gccggtagtg
     7201 cgcttgctcc agcgcgccgt gtttctcgaa gtgggcgagg atggcgcgaa tggccttggg
     7261 gtcttcgatg ctggcgacga tccgcaccgc gccgccgcag tgggcacagg tggtgacgtc
     7321 gatggaaaag acccgcttga gccgttgcgc ccagctcatc gcacggcgct tctgctcggg
     7381 gctgcgcggc tcgtcgtggg cgcttgcgtc aaccggcacc gcatcgcccg caggccgcct
     7441 gccgcgcccc gagggcgtca gctgcgcacg caggtttgca ttcggggcga atacgccgtg
     7501 gaagcgggtg agatgcgcgc gaggtggcgg gaccagtgcc gccagcttgg cgatgaagtc
     7561 caccgcatcc cattcgacat gcgtggtccc atttcgccac ggcgtcttga gctgataccg
     7621 caccctaccc tgcggtgaga tcgacagccg ctgctcgctg atcgccgggc gcgtgatgta
     7681 gcggcacagc ttttcgagct tgtggctttc gtgtgcttcc gcggccacgc cggcatgcag
     7741 cgagaagccg ccgaccttgc cggcgtcgcc ctccagcgag cctgcgtcac cgggcaatgt
     7801 ttgcagcgtg acgaccttgc ggccagcgtc gcgaccggtg gcgatgcggt aggtcatcga
     7861 actcatccgc agcccatcca tgccgtcgtc gctacccgcg ctgtcggaca ggaacacgga
     7921 ttcgtcttcg ccttcgagcc agccgcggcg cgacaggtgc cggcacacgc gatgcgcgat
     7981 ggtgccggcc agttccgtca gttgcgccga tgtgggcgca cgggtgcggt gcaggcgcgg
     8041 cttgcgctgc ggacgctcgg tggtgtcctc gtacacgccg tcgagccaca gcatgtggaa
     8101 gtgaacattc aggttcagcg cgctgccgaa gcgctggatc agggtcaccg cgccgcattg
     8161 cgcgctggcg tggtcgacgc cggcttgatc ggccaaccag ccggcgatca cacgatgcac
     8221 gatgcccagc accgggccga tggcctccgg cttgctggcg aacaggaagc gcagcgggta
     8281 cggaaaactc agcacccatt gccgcaccgg ccgcgggcca aacacctcct ccaccaggtg
     8341 ccgcgcgctc tcggccatgc gccgcgcccc gcagctgggg cagaagccgc gcttcttgca
     8401 ggagaaggcc accagcctct cggcccggca ctgctcgcac accacgcgca ggaaaccgtg
     8461 ctcgagcacg ccgcaacgca ggtaggtctc gaactcctcg cgcacatact cgggcagcgg
     8521 gcggtcttcg gcctcaagac gcgcgatgaa gtccgggtag tgcgcctgca ctagcgcgta
     8581 cagcagcgtg cgctcgggca ggtggcgcgc gtaacgcgca gcggcgtggg cggccggcag
     8641 tggcgcgcac acctcggcct gccgccgggg tgcggtcagg cgcggcacgc agcgctccgt
     8701 tgcagggacg gctgctcagg gttgcgcccg cgagcacgcg ttcatatcgg cgttttctga
     8761 tcttcgcgtc agacattgcc gcggggcggg taggaagtct cccgcctcgg acgccagaac
     8821 gcacaaagcc cggcacgagg ccgggctttg tggtcttgct tgatggatcg cggggcttag
     8881 aaatccatgc cgcccatgcc gcccatgccg ccgcccggag cggccggctc ttccttcttc
     8941 ggcgcttcgg ccaccatcgc ttcggtggtg atcatcaggc cggcgatgga cgccgcgttc
     9001 tgcagcgcgg agcgcgtgac cttggtcggg tccaggatgc cgaactcgat catgtcgccg
     9061 aactcgccgt tggcggcgtt gtagccgaac gcgccggtgc cttcggcgac gcggttcagc
     9121 accacgctcg gctcgtcgcc ggcgttggtg acgatctcgc gcagcggggc ttccatcgcg
     9181 cgcagggcga tcgcgatgcc gtggttctgg tcctcgttgg cgcccttcag ctcggcgatc
     9241 gcggccttgg cacggatcag ggcgacgccg ccgcccggga cgatgccttc ctcgaccgcc
     9301 gcacgggtgg catgcagggc gtcttcgacg cgcgccttct tttccttcat ctcgacttcg
     9361 gtggcggcgc cgaccttgat caccgcaacg cccccggcca gcttggccac gcgctcctgc
     9421 agcttctcgc ggtcgtagtc cgaggtggtc tcctcgatct gcgccttgat ctgcttgatg
     9481 cgggcttcga tgtccgcggt gtcgccggcg ccgtcgatga tggtggtgtt ctccttcgag
     9541 acctgcacct tcttcgcgcg gccgaggtcg ttgatggtcg ccttctcgag cgagaggccg
     9601 acttcctcgg agatcacggt gccaccggtg aggatggcca tgtcctccag catcgccttg
     9661 cggcggtcgc cgaagcccgg ggccttgacc gcgcagacct tcacgatgcc gcggatggtg
     9721 ttgaccacca gcgtggccag cgcctcgccc tcgacgtcct cggcgacgat cagcagcggc
     9781 ttgccggcct tggccacgcc ctcgaggatc ggcagcaggt cgcggacgtt cgagatcttc
     9841 ttgtcgtgca gcaggatgaa cggatcctcc agctcggcct gcatcgactg cgggttgttg
     9901 atgaagtacg gcgacaggta gccgcggtcg aactgcatgc cctcgacgac gtcgagctcg
     9961 ttctccaggc ccgagccctc ctcgacggtg atcacgcctt ccttgccgac cttgtccatg
    10021 gccttggcga tcagctcgcc gatgtcggtg tcggagttgg ccgagatcga accgacctgg
    10081 gcgatctcct tgctggtcga gcagggcttg gagatcttct tcagctcctc gacggccgag
    10141 gtgacggcct tgtcgatgcc gcgcttgagg tccatcgggt tcatgccggc ggccacggcc
    10201 ttcatgccct cgcggatcag ggcctgggcc agcacggtgg cggtggtggt gccgtcgccg
    10261 gcgttgtcgg aggtcttgga agcgacttcc ttgaccatct gcgcgcccat gttctcgaac
    10321 ttgtcggcca gttcgatctc cttggcgacg gacacgccgt ccttggtgat cgtcggggcg
    10381 ccgaagctct tctcgagcac gacgttgcgg cccttcgggc ccagggtcgc cttgacggcg
    10441 ttggcgagca cgttcacgcc gcggaccatg cgggcgcggg cgtcttcacc gaaacgaata
    10501 tccttggcag ccattgcgga attacctcag ttctgtgatt gcgggttggg atgcggcgaa
    10561 gcggcgggct cagcccagca cggcgaagat gtcgtcttcc ttcaccacca ggtactcggt
    10621 gccgtcgagc ttgacctcgg tgccggcgta cttgccgaac agcacctggt cgccgacctt
    10681 gaccttcggc gcgcgcacgt tgccgttgtc gaacaccttg ccctcgccca cggcgacgac
    10741 ctggcccttg atcggcttct cggcggccga atccgggatc acgataccac cggcggagac
    10801 cttctcttcc tcggtgcgct tgatgacgac gcggtcgtac agcggcttga tgttaatcgc
    10861 cgcgcatatc ttcctcgtgg ggctacccat cgcctttatc gcgcgccgcc gcgaccgtgc
    10921 gcgcgcgcaa aaattcgccg cctgatcgcc tcgggcaatc atccttgtcg agggtgccaa
    10981 gctgttcttt actgaagaac gtcccgacat ccttgttgaa gaggcggcgg gcttgtgggc
    11041 ctgatgctat gcggctgggc gagagggagg aatatatgtc cggaataatc gacgactcag
    11101 tcgcggcgtt gtttcggctt cagcgctgcc gccgctggcg cggcgtggcg cgcctcgagc
    11161 cattcgatgc cgaaggaaac gatatcggcc gcgtcgatca gccgggattg cgcgccgccg
    11221 accgggcctt gcgcaaccct ggtgcgggcg agatagtcgc gggcgatccg ttcgaccgcg
    11281 tcggggccgt cgggcgcggc atattcgtcg aggatgccgt tcgaatccca gtcggacgtg
    11341 gtgacccaga cgcgcttgcc ttcgcgcagt aagggcatcg aataagtgac gcggcgcttg
    11401 ccctcgatcc gcgccaccgc ttcggcataa tggagcgcgg tgactgcatc gggcccggcg
    11461 ccgatcgaca ggatttttcc tgcgtgggcg agaaaacgcg cgatcggcga gcgggggcca
    11521 taagcggcgc ccatctcgtg cggcgccacc agccacgccg catcgggccc gatcgccgcc
    11581 atcgatgcgt cgggatgggc gctgcgccga caccccggat aggttcggat aaattcgttg
    11641 atcgcgccaa agccggggta ggcgggcgca tggtcggggt cgaacgcagg ccagctttgg
    11701 gcgatggcgg cgggcggcgc atcatgaccc agcgtctgtt catagggcga atcgcgccac
    11761 gagacgaagg cgagaatatt cccggtgggg ccgacggcct cgatcagcgc atcgacaagt
    11821 gtctgcggtc cgtccgccag cggcccgacg gcgcgcaacg acacatgcgg catcaccata
    11881 tcgcctgagc gcacgccgag gtcgcgcaat tgccggacca gctcgctttt ggaccagggc
    11941 gtcgcgggtg ccgtcgcggg ttcgtgcaaa tcgccgtttt tgcggggatc agtcatcatg
    12001 atatatctcc caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact
    12061 tgacctgata gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag
    12121 ccgcgccgcg aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt
    12181 ggtgatctcg cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc gggaggccaa
    12241 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
    12301 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
    12361 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
    12421 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
    12481 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
    12541 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
    12601 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
    12661 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
    12721 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
    12781 aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc
    12841 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
    12901 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt
    12961 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
    13021 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa
    13081 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
    13141 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga
    13201 acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc
    13261 aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt
    13321 ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct
    13381 gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc
    13441 ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg
    13501 tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt tgcaactgcg
    13561 ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca agggctccaa
    13621 ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc agctgtcgcg
    13681 tgcacgggca tggtggctga aggaccaggc cgagggccgc agcggcgttg cgcttcccga
    13741 cgcccttgag cggaagtatc cgcgcgccgg gcattcctgg ccgtggttct gggtttttgc
    13801 gcagcacacg cattcgaccg atccacggag cggtgtcgtg cgtcgccatc acatgtatga
    13861 ccagaccttt cagcgcgcct tcaaacgtgc cgtagaacaa gcaggcatca cgaagcccgc
    13921 cacaccgcac accctccgcc actcgttcgc gacggccttg ctccgcagcg gttacgacat
    13981 tcgaaccgtg caggatctgc tcggccattc cgacgtctct acgacgatga tttacacgca
    14041 tgtgctgaaa gttggcggtg ccggagtgcg ctcaccgctt gatgcgctgc cgcccctcac
    14101 tagtgagagg tagggcagcg caagtcaatc ctggcggatt cactacccct gcgcgaaggc
    14161 catcggtgcc gcatcgaacg gccggttgcg gaaagtcctc cctgcgtccg ctgatggccg
    14221 gcagcagccc gtcgttgcct gatggatcca acccctccgc tgctatagtg cagtcggctt
    14281 ctgacgttca gtgcagccgt cttctgaaaa cgacatttag atcgatatgt gataaattgg
    14341 cctgccagtt taagatgggt gcatttgagt atgcccaaag gagcccgcaa gtatgcgcag
    14401 gacgaagcca gtagccgcgc cgatggtggc gcgggtctat ctgcgcgtca gcaccgacgc
    14461 gcaggacttg gaacgccaag aggcgatcac tacggccgcg aaggccgccg gctactacgt
    14521 cgccggcatc taccgtgaga aggcatccgg cgcacgcgcc gaccggcctg agctgctgcg
    14581 catgatcggc gacctacagc ccggcgaggt ggtcattgcc gagaagatcg accgcatcag
    14641 ccgcctacct ttgcccgagg ccgagcgcct ggtggcctcg atacaggcca aaggcgcacg
    14701 cctggccgtc cctggcgtgg tcgatctatc cgacctggcg gccgaggccc agggcgtcgc
    14761 caagatcgtg ctggaagccg tgcagatcat gctttttcgc ctggccttgc agatggcccg
    14821 cgacgactac gaggacaggc gcgaacgcca gcgccaaggc attgagttgg cccgccaggc
    14881 cgggcggtac aagggccgcc gtgctgatcc gaagcgccgc gcccaagttg tcgcgctgcg
    14941 caagtccggc tacagcatca acaagaccgc cgagctggcc gggtacagtg cggcccaggt
    15001 gaaacggata tgggccgagg tcagccaggc cgaagcgaag cagcacggcg cgttcgtgga
    15061 ggacgcattg acggaagccg atgccctggc cgctgtcggc caggatgagc gccaggagga
    15121 aagggcatga agaagccgaa ccaagacgac gagccgtttt tcatcaccga ggagattgcg
    15181 gccgaaatga tcgccggcgg ctatgagttc gagctgccgc ccattccttg caccatccgc
    15241 ctacgcgacg tgctggagcg catgaccgat gctgagctag cattgcagcc gggcgagatc
    15301 gccgaccagg agcgtgaacg ctgccggcgc aagccgtgtt caacctcatg atctggtcat
    15361 ggtatttttc atggcactga gcctgatagt tcttgcaaat tgttgtcact aaagggtttt
    15421 gtgtgcttgt ttacaatcga gtgggagtga cgggcactgg ctggcaatgt ctagcaacgg
    15481 caggcatttc ggctgagggt aaaagaactt tccgctaagc gatagactgt atgtaaacac
    15541 agtattgcaa ggacgcggaa catgcctcat gtggcggcca ggacggccag ccgggatcgg
    15601 gatactggtc gttaccagag ccaccgaccc gagcaaaccc ttctctatca gatcgttgac
    15661 gagtattacc cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt gccgggctat
    15721 gtgcaacggg aatttgaaga atttctccaa tgcgggcggc tggagcatgg ctttctacgg
    15781 gttcgctgcg agtcttgcca cgccgagcac ctggtcgctt tcagctgtaa gcgtcgcggt
    15841 ttctgcccga gctgtggggc gcggcggatg gccgaaagtg ccgccttgct ggttgatgaa
    15901 gtactgcctg aacaacccat gcgtcagtgg gtgttgagct tcccgtttca gctgcgtttc
    15961 ctgtttgcca gccggcccga gatcatgggg tgggtgctgg gcatcgttta ccgcgtcatt
    16021 gccacgcacc tggtcaagaa agcgggccat acccaccaag tggccaagac gggcgcggtc
    16081 accctgatcc agcgttttgg atcggcgctc aatctgaatg ttcacttcca catgctgttt
    16141 ctcgacggtg tgtatgtcga gcaatcccac ggctcagcgc gtttccgctg ggtcaaggcg
    16201 ccgaccagcc cagagctcac ccagctgacg cacaccatcg cccaccgggt gggtcgctat
    16261 ctggaacggc aaggcctgct ggaacgggat gtcgaaaaca gctatctggc ctcggatgcg
    16321 gtggatgacg acccgatgac acccctgctg gggcactcga tcacttaccg tatcgctgtc
    16381 ggttcacagg cggggcgaaa ggtgttcact ttgcaaactc tgccgaccag tggtgatccg
    16441 ttcggtgacg ggattggcaa ggtagccggg tccagcctgc acgccggcgt ggcggccagg
    16501 gccgatgaac gcaagaagct cgaacggctg tgccggtaca tcagccgccc ggcggtatcc
    16561 gagaagcggc tgtcgttaac acgaggcggc aacgtgcgct accagctcaa gacgccgtac
    16621 cgggacggca ccacgcacgt cattttcgaa ccattggatt tcattgcaag gctggccgcc
    16681 ctggtaccga agcccagagt caacctaacc cgcttccacg gggtgttcgc acccaacagt
    16741 cggcaccggg cgttggtcac gccggcaaaa cggggcaggg gcaacaaggt cagggtggct
    16801 gatgaaccgg caacaccagc acaacggcga gcgtcgatga catgggcgca acggctcaag
    16861 cgtgttttca atatcgacat cgagacctgc agcggctgcg gcggcgccat gaaagtcatc
    16921 gcctgcattg aagaccctat agtgatcaag cagatccttg atcacctgaa gcacaaagcc
    16981 gaaaccagcg ggaccagggc gttacccgaa agccgggcgc caccggctga gctgctcctg
    17041 ggtctgtttg actgacgagc ctgaaggcca acgataccaa tcaaaatgct gcgttcacag
    17101 cgccgcggca gggatccgcc gtgctggttg tcggaaaagg agccgctagt gggaaagagg
    17161 agggtaaatt ttcagcgttg ctggctcccc gtcagccgga ttgggttgca tcgcaggggt
    17221 gtcgaaagag tcaactgcgg tccaaagctg ttggacttgg gtgaaaaggg cgtttattct
    17281 tcctatacgt tgtcggcagc gggccaaaaa ggaatacgtc catgcccatc gaggtgaaac
    17341 cggctgtgag cgcgggttca agcatatagc ccgacaggcg cgtatccttg ccgatcacga
    17401 cacgatggcg gtggtcaccg cgacgaaaga cacggccagc cgccatgccg acgcgcaagg
    17461 cggtttccgc cgtcatcgcg ccttcgttgg ctttgccacg aataccgtct gtgccgaaat
    17521 atttgcgcac cataaggtcg attatcctgt cgtcgggtcg ccctcaaagg ggacatgcct
    17581 gctgaaccgc gaatatagag aaatatcccg aatgtgcagt taacgaattc ttgcggtttc
    17641 tttcagcgcc gccaataccg ccagcccgtc gcgcaagggg cgcggctcgt gtgtgcggat
    17701 gaagtcagct ccacctgcgg cggcggcaag ctctgcagcg agtgtcgcgg ccccgacatc
    17761 ccccggacca cggcctgtga gcgcgcgcag aaaggatttg cgcgaaacag acagaagcac
    17821 cggcaaatcg aagcgcagcc gcaattcatc gaaccgcgcc agcaccgaga gcgaggtttc
    17881 gggagcagcc cccagaaaaa accccatgcc gggatcaagg acaaggcggt tgcgtttgat
    17941 accggcaccc gtcagcgccg cgatgcgcgc gtcaaagaac gccgcaatgt gatccatgat
    18001 gtcgccagcg ggtgcctcgc gccgatctgc ctgcccgtct tgcaccgaat gcataacgac
    18061 gagtttggca gatgatttcg ccaattgcgg atagaacgca gcgtctggaa aaccgcgaat
    18121 atcattgaga taggccacac cacgcgacaa ggcataggct tgcgtcgcgg gttgataact
    18181 gtcgagcgag acgggaatgc catctgcctt gagcgcgtcc agcaccggcg cgatacgcgc
    18241 gatttctgtg tcggacgaaa caggcgcggc gtcgggattg ctggatgccg gaccgaggtc
    18301 gatcacatct gccccctcgg ccatcagctt acgcgcctgc gcaatggctg cgtctggcgc
    18361 cagataccgg cctccatcgg agaaactgtc cgaggttatg ttgacgatgc cgaaaatgat
    18421 gagcgattta ttcatggggg cttctataat aataataatc gagcatgagt ctcatacgga
    18481 tgctcgggtc gaaagggaat ccccaggcga gtaacctgtt tgcggtgatc cattagctgc
    18541 aggagcagaa tagcatacat ctggaagcaa agccaggaaa gcggcctatg gagctgtgcg
    18601 gcagcgctca gtaggcaatt tttcaaaata ttgttaagcc ttttctgagc atggtatttt
    18661 tcatggtatt accaattagc aggaaaataa gccattgaat ataaaagata aaaatgtctt
    18721 gtttacaata gagtgggttt ccggtgctcc tttgacactc tctccatatc cctcaggccc
    18781 ggcaccaaca gataccacgt tattttctcc cgacacctgt gagccatggc ccagagctac
    18841 agagcctgaa gaattttcta ttccagccca tcccccaata gcaacactct gctgtgaatt
    18901 aagtacctca gcgctcttac ctatggcaac accatatttt gactcagcct gaccggcatc
    18961 ccttcccagg gtgacactgt gtggagaatt atacactccc gaaaaagagc caaactgaag
    19021 acttcctggt gaatccatgt taaaagcgcc acctcccaaa aggaaagtgt tattagctgt
    19081 accagttgaa ttcgtaataa aagagttttc tccaatcagt aagctggaag aattagcatc
    19141 aacaggccct gtcaaaacag cctgagcctg agccgagagc agcagggcca cactcactgc
    19201 cagcgcgctt ttctttaaaa aagcatcacg tcttttaagc
//
LOCUS       DBBGLQ010000082        19006 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.082, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000082 DBBGLQ010000000
VERSION     DBBGLQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.082"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(2..346)
                     /locus_tag="ACIYSB_003819"
     CDS             complement(2..346)
                     /locus_tag="ACIYSB_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGC5430769.1"
                     /translation="MFVPKRGDICWLDFEPQKGKEIGKYRPALILSHEQYNRLTGLVI
                     CCPVSTSIRGAPTEVPVTNLDAPSVVATTLIQTLDWRQRQVRLITEAEEGVFDEVLER
                     VITLIGGEHLLK"
     gene            complement(346..594)
                     /locus_tag="ACIYSB_003820"
     CDS             complement(346..594)
                     /locus_tag="ACIYSB_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5430770.1"
                     /translation="MRTQVTLQKWGNSIALRLTGNLKTVPGFSAGDVVDVEISEKGLI
                     VEKPVRPRLTEAVLLAGLTPFSAHADEAATLLDKESEF"
     gene            complement(663..3365)
                     /locus_tag="ACIYSB_003821"
     CDS             complement(663..3365)
                     /locus_tag="ACIYSB_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HGC5430771.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEVKGEV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLLTLENIRKLNGGWQSVPKDIFTQVVPQSRFSG
                     HRMDMHPVSDHEWHRMRTGAGPQGAIKREIFSDKESLWGYAVSHCARDIQYMISEGRF
                     SWQNCHELFARSGLMLQKLHHGLVIVDAFNHDQTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFDRVKPESRYNPDMAVSDSKEPGFKRDPELRRRRRETRAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREVHAACRRRKAHIRVQFRDPQVRKLHYHIAEVQRMQALIRLK
                     ESVREERLSLMAEGKWYPLSYRQWVEQMAVQGDSAAVSQLRGWNYRDRRNDEVLLTTP
                     QRCVVLCEPGSTPIHRDIGGMVATLQRNGSVRFRDATTGEYVCTDYGDRVVFSNSADN
                     KTLKKRMVRVSSVLFARDPRMGFEPEGQDAQFNRMFADMVAWKNVQRDERKGHYRISR
                     SDVDQLRVSSEKRFRDYLKGRDTESHTYHHATDENKWEPPSPGM"
     gene            complement(3377..3709)
                     /gene="mobA"
                     /locus_tag="ACIYSB_003822"
     CDS             complement(3377..3709)
                     /gene="mobA"
                     /locus_tag="ACIYSB_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HGC5430772.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            3942..4277
                     /locus_tag="ACIYSB_003823"
     CDS             3942..4277
                     /locus_tag="ACIYSB_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HGC5430773.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(4363..5211)
                     /locus_tag="ACIYSB_003824"
     CDS             complement(4363..5211)
                     /locus_tag="ACIYSB_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGC5430774.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLSEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            5560..5757
                     /locus_tag="ACIYSB_003825"
     CDS             5560..5757
                     /locus_tag="ACIYSB_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430775.1"
                     /translation="MQGAPHPARLKKNDGTTMACRGHWRTTPAQVKTEKTARHLQLEK
                     VKFLHFFTLIYRTDLAIVMIK"
     gene            5791..6042
                     /locus_tag="ACIYSB_003826"
     CDS             5791..6042
                     /locus_tag="ACIYSB_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGC5430776.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(6073..6291)
                     /locus_tag="ACIYSB_003827"
     CDS             complement(6073..6291)
                     /locus_tag="ACIYSB_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430777.1"
                     /translation="MTIAERLIQKGFDEGFKEGFKEGFKQGFKQGALEVAREIACRLR
                     DIGWSPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(6353..7231)
                     /locus_tag="ACIYSB_003828"
     CDS             complement(6353..7231)
                     /locus_tag="ACIYSB_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGC5430778.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDTVSPEFMRALAERLPQHEDKLMTIAERLEQKGALEKALAIARQLQKMGMTPEQI
                     KQATGLSEAELKKVIH"
     gene            complement(7296..7562)
                     /locus_tag="ACIYSB_003829"
     CDS             complement(7296..7562)
                     /locus_tag="ACIYSB_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430779.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(7654..8088)
                     /locus_tag="ACIYSB_003830"
     CDS             complement(7654..8088)
                     /locus_tag="ACIYSB_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HGC5430780.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            8207..8815
                     /locus_tag="ACIYSB_003831"
     CDS             8207..8815
                     /locus_tag="ACIYSB_003831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430781.1"
                     /translation="MGQRLPRHGAVVRGQTQKFLTPAAKSFVAGTSLPWTAETCCGRF
                     SPRKVHSGKQAAGVAAEGLTAPSGAVALHTVRCAGSAVSGATGAARPERSLQAWFFRH
                     RTVAAARNRASGFGGDWRVRQQLPVGVGPTTGCRQAAGSRRVSPPPGIRTTETTKQKN
                     ASHHDGQEGQRRRRGPPVFQAGVSLPGFSGGPAGHTPAGAGE"
     gene            complement(8817..9317)
                     /locus_tag="ACIYSB_003832"
     CDS             complement(8817..9317)
                     /locus_tag="ACIYSB_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGC5430782.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEDDSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(9782..10378)
                     /locus_tag="ACIYSB_003833"
     CDS             complement(9782..10378)
                     /locus_tag="ACIYSB_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430783.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGENDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISRALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMSVGNESPPAQPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVSITDVLR"
     gene            complement(10375..11095)
                     /locus_tag="ACIYSB_003834"
                     /pseudo
     CDS             complement(10375..11095)
                     /locus_tag="ACIYSB_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(11092..11526)
                     /gene="psiB"
                     /locus_tag="ACIYSB_003835"
     CDS             complement(11092..11526)
                     /gene="psiB"
                     /locus_tag="ACIYSB_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGC5430784.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(11581..13539)
                     /locus_tag="ACIYSB_003836"
     CDS             complement(11581..13539)
                     /locus_tag="ACIYSB_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGC5430785.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDAGRTEQVQEKASVEGISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLEIAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFYMKD
                     NRWVPGWMCPPRPQTDATEHTANLADAA"
     gene            complement(13603..13836)
                     /locus_tag="ACIYSB_003837"
     CDS             complement(13603..13836)
                     /locus_tag="ACIYSB_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HGC5430786.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(13894..14421)
                     /locus_tag="ACIYSB_003838"
     CDS             complement(13894..14421)
                     /locus_tag="ACIYSB_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGC5430787.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPESQPQ
                     PPEGEDYGFSDDIPF"
     gene            14790..14858
                     /locus_tag="ACIYSB_003839"
                     /pseudo
     CDS             14790..14858
                     /locus_tag="ACIYSB_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(15192..15383)
                     /locus_tag="ACIYSB_003840"
     CDS             complement(15192..15383)
                     /locus_tag="ACIYSB_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430788.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(15380..15802)
                     /locus_tag="ACIYSB_003841"
     CDS             complement(15380..15802)
                     /locus_tag="ACIYSB_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGC5430789.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(15849..16274)
                     /locus_tag="ACIYSB_003842"
     CDS             complement(15849..16274)
                     /locus_tag="ACIYSB_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGC5430790.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(16689..17459)
                     /locus_tag="ACIYSB_003843"
     CDS             complement(16689..17459)
                     /locus_tag="ACIYSB_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430791.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDTRRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            complement(17510..18027)
                     /locus_tag="ACIYSB_003844"
                     /pseudo
     CDS             complement(17510..18027)
                     /locus_tag="ACIYSB_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(17957..18178)
                     /locus_tag="ACIYSB_003845"
     CDS             complement(17957..18178)
                     /locus_tag="ACIYSB_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430792.1"
                     /translation="MNYAGHEKLRAEVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(18179..18862)
                     /locus_tag="ACIYSB_003846"
     CDS             complement(18179..18862)
                     /locus_tag="ACIYSB_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGC5430793.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
BASE COUNT         4174 a         5626 c         4918 g         4288 t
ORIGIN      
        1 ttcacttcag taaatgttca ccaccaatca gagtgatcac tctctcaagc acctcatcaa
       61 atacgccctc ttctgcttcc gtaattaaac gcacttgcct ttgccgccag tcaagtgtct
      121 gtatgagcgt tgtggccacc acacttggtg catcgagatt agtaacagga acctctgttg
      181 gagcgccccg gatactcgta ctgacagggc agcatatgac aagccctgtt aaacgattat
      241 attgctcatg ggacaggatt aatgccggac gatatttgcc aatttctttt cctttttggg
      301 gttcaaaatc aagccagcaa atatcaccac gctttggaac aaacatcaga actcactctc
      361 cttatcaaga agagtggcag cttcatctgc atgcgcggaa aatggcgtca gccccgcaag
      421 caatacagcc tctgtaagac gaggtctcac cggtttttcc acgatcaatc ctttctcact
      481 gatttccaca tcaaccacat ctcctgccga aaaacccggc acggttttca gatttccggt
      541 taaccggaga gcaatactgt tgccccattt ttgcagtgtc acctgtgttc gcatatctcc
      601 cccataatgt atacacaacg aggacacatt acggtgattt aatcaccctg tctacatgat
      661 ttttacatcc ccggtgatgg cggctcccac ttattttcat ccgttgcatg atggtaagtg
      721 tgtgattcag tatcacgccc tttaagataa tcacgaaaac gtttttcact actgaccctc
      781 agttgatcaa catcagaacg acttatcctg tagtgcccct tacgctcatc ccgctgcacg
      841 tttttccacg caaccatatc agcaaacata cggttaaatt gtgcatcctg accttccggc
      901 tcaaacccca ttcggggatc ccttgcaaaa agcacactgg acaccctgac catccttttc
      961 ttcagtgttt tgttatcggc actgttgctg aaaacaacgc gatcaccata atctgtacag
     1021 acgtactctc ctgttgtcgc atcacggaaa cgcacactgc cattacgctg caaagtcgcc
     1081 accatgccac cgatatccct gtgaatcggg gtggagcctg gttcacaaag aaccacacag
     1141 cgttgaggcg tggttaaaag cacctcatca ttccttctgt cgcgataatt ccatccacgt
     1201 aactgagata cagcagcaga atccccctga accgccatct gctccaccca ctgcctgtaa
     1261 gacagcgggt accacttacc ttccgccatc agcgacagtc gctcttccct gacgctttcc
     1321 ttcagcctga tcagcgcctg cattcgctgc acttccgcaa tatggtaatg cagcttacgc
     1381 acttgcggat ccctgaactg tacgcggata tgcgccttgc gccggcggca ggcagcatga
     1441 acttcccgta accgttcgcc gtaacgtaaa tcgggtttac gccagtgctc cttccatgca
     1501 aggtaacgcg cacgtaaatc ttcccgcgca gctgcacggg tttcacgacg acggcggcgc
     1561 aattcaggat cccgtttaaa cccgggttcc ttactgtcac tgacagccat atcagggtta
     1621 taacggcttt caggcttcac ccgatcgaaa atatccgccg ctgcaatttc aaatggcccg
     1681 gcctgcggct ctgctctgga caaagtcaaa tccggatgaa tgctgctggc tttaaccggt
     1741 gtctggtcat gattaaacgc atccacaatc acaagaccat gatgcagttt ctgtaacatc
     1801 agtccactgc gggcaaacag ttcatggcag ttctgccagc taaagcgccc ttcagagatc
     1861 atgtactgaa tatcacgggc acaatggctg actgcatatc cccacagact ctctttgtca
     1921 gagaaaatct ctcgcttaat tgccccctgt gggccagccc ctgttctcat tctgtgccat
     1981 tcatgatcac tgaccggatg catatccatt cgatgcccac taaagcgact ttgtggtaca
     2041 acctgagtaa aaatatcctt cggtacagac tgccagccgc cattcagttt tctgatattt
     2101 tccagcgtta acaacggcca cacactgtct gcacgtactg gtgtctgttg tttatcaaat
     2161 ccatcatgca gtaccagatg cccgtgctgg atccccatcc ataatcccgc ctctgccaga
     2221 gtacggtgta cagccaggca gcttttcagt tcaccattgc gcgccattgc cggtaagcgt
     2281 tcggcaaaat gacggcaggc gtactgtttc agactctccg tttctgccac tttttccggc
     2341 cggatatcaa cgtttatggc ttccggatca taacgaccgg gtgtccccac ctgactgaaa
     2401 atatctgtcg gtacaggctg atattccccc agtttccgcc ccagtttttc agctgtccat
     2461 gacggtccga acgaactgag tgcaacacct tcccgcgctc tgtcccagcc atccttcacc
     2521 accagttccc cttcctgcat cgtgatatac agaccatcgc tggccagccg tttatgcaaa
     2581 gataaccagt cctgtgcagg ctcttcacgc agcccggcaa tcgacatctg agcaatatat
     2641 tccctgaacg tctgcttttt cccgcgtcgc caggcattac ggcgctcacg taccagcgct
     2701 gttttgcgca caatacggtt gccgggtgca tgcacaaagc agccattatc cggcgcgaat
     2761 ccgtgcttaa gttcaagttc acgacatgca cggctgagtt tttcctggct ccacggaagg
     2821 caattgatat agccggtttc agggtggaca cgattcactg caacatgtac atgcaggtta
     2881 tccgtgtctg tatgaacggc tgccacatac tgatgttttg acagctcaag ggatttcagg
     2941 gtgtgacgca cacagtcaaa aagctgctca gaacgcggac tttcgtgcgc aggccaggag
     3001 agaatatagt gaaaaacagg atctgtatca tctttggaaa aatgagcaag gtctgctgct
     3061 ttctgcattt cttcagccgc agattcaaga gacaaacagt tatgaaagca ggtcacacca
     3121 tagaaattca cccactgact gccgtctttc atcacatcaa tcagggaaac aaatttttca
     3181 ttacgcagac gtgttgcata atcaacaaga cgtctgaagc gattccggtg cggaacctca
     3241 cctttcactt ccgtgtctgg tctgagttca tcttccggca catcatcccg cactgaggtg
     3301 tacgcaatca ggtcttcaaa agaagacttt ccgtcgcgtc tttttttcgg aatgatggca
     3361 ttcatcagcg attatctcag cgtcccatta aatctcttcg cagagtatta gcaagttctg
     3421 taatagcaac gagaacctct gcatactctt tatctcccgt gcgttttcct tccacaaaca
     3481 gatgcttctg caggcgacca agacgcatca gctctttcaa aaaatcttca tcaatgcggg
     3541 catttatctg ttttcccagc acagtttttc ttatcagagt tgaaactgtc agtccgtttt
     3601 tttctgcctt ttctttaaca atctcagcct cttctggtga aaaacgaaca gcaatgagga
     3661 cattcttttg ccgtttctca ctgccacttc gggttttctt ctcactcatg gtctgctcct
     3721 taccggggtg tcggggctaa gccctgacca ggtggtaatc gtatagccgt gcgtgcgcgg
     3781 ttatacgatt acacatcctg tcccgttttt cagtccatta tagcctgtta tttttgtgca
     3841 tcatagtctt atgcagatgg cagaggcagg gcgcagccct gccaggagtc agtttttgat
     3901 gataaaatca gacttcaaca tgaaacggag agaaagtgcc aatggcttct aaaaaatttt
     3961 actcagatga tgacatccag ctggcaaaag ctgctttatc ggaattgcca gatctcaccg
     4021 ctcaacggaa aacattgcgt gattttcttg atgccattcg tgatgacatt attgtcctgg
     4081 taaggacaaa aggttataca ctggcagatg ttcgtgacac gctcaaaaat gcagggtatg
     4141 aagtaggaga gaaggcactt cgtgacatta ttcgggaagc agaatcgaaa aaaccatccc
     4201 gccgttcttc ctctaaaaca gcacctaaaa agacagattc aggcaggaaa gatggcatcg
     4261 atatgaacaa caattgatgt gataatgtca catcgtgttc tgagattatg atgattacag
     4321 aaaaaaaagc cccggcataa ataccagggc ttttctgttt aatcagactc gtggcggcaa
     4381 tgcaccagga taatgccgtg caataatatc attcagtttt tcaacaaggt cagaacgctt
     4441 aaagacaatg tgtacggtcc ctttctggta gccacgcacc gtaaaatatt ccccttctga
     4501 cagcttaccg acattgtcgc cgttcccgag aaattcacca tactgcgccc cgatgctgct
     4561 ccggaagtca ggaacgttgc gcccctccag cagataaaaa ggacgtacca ggtcatccag
     4621 tgcctcccgt ccgctgcagt ccagcgtcac acgcccgtta ctccagcggt acagcaggtt
     4681 ttcaataatg attttcttgc ccagacggca gggattattg gttctgtaat cccatgacaa
     4741 tttccggtag acatcaatca gtccctgttc aaatgtctgc atcttgctgg cattcagatg
     4801 gcggaatgtt gccagtacat tgtccagggt gatttcagga caggtgtcgc tcatcagctg
     4861 gctgttccac tcatcacgct ggcatgaact catgaaggtg tacatgcccg tatctgtcat
     4921 caggcgatcc cagattttcc ggtcggcaag acgggtgata ttcttttttg cttcttccgg
     4981 tttatccatg catcgaacgg agtcagtcac gcagtcttca aacccgtaga gaaaaatgtc
     5041 accggaagca tcgagcagaa tcctgcgcgc tgtcgccagg cattccatcg cctgcatgaa
     5101 cagtgcaata ccttcgttac gctgcgcgat gatgcggtcg atagccacag acggaataac
     5161 atctgtcgtg ccggcagtat tcaggtcagc agtgggtaaa gtctgattca tcacattgtc
     5221 tcctcatcgt cacgactgcc gggtcagcca ggtcatttcc cggaggagcg aagctccttc
     5281 tgccgtccgg gtcttcgggg atttccccgc agtggacctt tccgacgggg gacgctcacc
     5341 acgtcacggg gcagttgtcc gggggcagac gcaaaagttt ttgacgcctg cggcaaaaag
     5401 ttttgtggct ggtacatctc tcccctggac gactgaaacg ggatgtggtc gtgtatgtac
     5461 ccccggaaag tccgtctgcg gaaaacgggc cgcagtgcgg cggcagacag gcagagacag
     5521 ctgcgccatc aggcgcagtg gccttacaca ccgtcaggtg tgcagggagc gccgcatccg
     5581 gcgcgactga agaaaaacga cggcacaaca atggcatgtc gtggtcactg gcgcaccact
     5641 cccgcacagg tgaaaacaga gaaaacagca cgacatctgc aactagaaaa agtgaaattt
     5701 cttcactttt tcacacttat ttatcgcact gatttggcaa ttgtgatgat aaaataaagt
     5761 gaattttttt cactttttca aaggtgtttt atgaccggct gggaactgcg aatctggcga
     5821 aaaagcatgc tctggtcacg cgaaaaagcg gccagagaat ttggcgtgac gcagagaaca
     5881 tggcacgcct gggaaaatgc ggaacaggtg gacgtcaccg tctggaggac cacccaggca
     5941 ctttcagtcc gtgacctgct gcctcacatg caggggatgc gtaaggcaga tatcatcagg
     6001 cgactggaaa atgaactggg ggaaacggca gaggatgtgt gagcgtgatt cacagcctgg
     6061 ctgaatgcca ggctactgct catcaggaaa cagctttttc agttcttcac cggaaagtcc
     6121 cgtgacctcc tgaatccgtt ccggcgacca gcctatatcc cgaagccggc aggctatttc
     6181 ccgcgccact tcaagagctc cttgtttaaa accttgttta aaaccttctt taaaaccttc
     6241 tttaaaaccc tcgtcgaaac ctttctgtat aagacgttct gcaatcgtca tgctgtttgc
     6301 tctgctcctg ctggcaaaaa gcccggcatt ttgccgggct gtgatgctct catcagtgaa
     6361 tgactttttt cagttcagct tcggaaagtc cggtggcctg cttaatctgc tcaggtgtca
     6421 tccccatttt ctgaagctga cgcgcaatgg ccagggcttt ttcaagcgca cctttctgct
     6481 caagacgttc tgcgattgtc atcagtttat cctcatgctg cggcaggcgc tcggccagcg
     6541 cccgcataaa ttccggtgat acagtgtccc ctgcgttaac gatgtaatga atcagaacac
     6601 gcacctgctc gtctgaaatt tccaccatga cctgcgggag tttgtccagc agctccatca
     6661 tgtcacgatg gcggatatgc ttcatcagca gcgtcagtgc ggccatccgg cgatggttca
     6721 tgatttcatt gtcatcaacg acagtgatat caaccagcgg aaacggcttt gtgtatattt
     6781 tagccgcaag cgccggatcc tcaaaacagt ccagccagtt catgctgtac gggtacgggc
     6841 tgcgctcacc gtgatagaac agcaccggaa tcaccagtgg caacgttttg tgctgctcca
     6901 gatgtctctg catcgccgcc acactgtagc gtaacatgcg aaaaggcatg tacagatttt
     6961 cggtgctctg gtgctcgatg agcgtataaa catagccgtc ttcacccccg gtggttttca
     7021 cactccagag gatatcgctg gcgtactgat gcaggtccgg ctcaacaaag gtggcgggtt
     7081 ccagcttcag ggtggacagg tcgcacaact gccggtactc cgcaggaaga tgcaactcca
     7141 gaaaatccct ggcaacgtcg ggattcgcca ggaacgaccg gaacgcggca tcatgcggcg
     7201 tgggcgttgt ggtgtttttc ttcttactca cggttcctcc tggtgagatg tctgaacgta
     7261 tcctgccgcc tgcacaggca gacggcaagg gttaatcagt gacagacccg ttcaatttca
     7321 agcgcgcgga gcaggtggcc ccattcgctg actttcatgg cggggatacg cacccgcttc
     7381 tgctcactgc agcgtctggc gatatgaccg ctcatcgcca ggacgtccgg gttaatctca
     7441 tcctgtgcca gctcctgacg tctgaagcgg gtatgacggt gcaggggttt attctcccgc
     7501 aggcactctt cacgcagacg ctttacatac tcggcggttg cgaagataat tgatggtctc
     7561 acaaatattt ctccttatgt tgttgatgac tgccgctctt cctggcgggg aagtaacggc
     7621 ggagagaggc agggtttatc gccctgcctt ttttcatttc ggacagcgac gcagtgccag
     7681 cgcgagcgct ttcatcttcc agccttccgg cgtgcggtac atcaccttcc agtagccgtc
     7741 gatgaacaca taatggaagc gcacctcttc tgtgctttca gtggcaatag cctccctgaa
     7801 agagcggtac tcacgcgctt cactgtccat ccggaaacca ctgtcacgtc cgtaataagc
     7861 cgtcacaccg tccaccgggt tctgaagcgt atgcccggcg ggtttgtcgc aacggggacc
     7921 gaggcaacgg atatctccgt gttcaaccag ggcttcggcc agctgaccgg tggcatagtg
     7981 ggtatgcagg atgcgaccgt tccaggtcgg ctcaccgtgc aggtggcagt aaacggtacg
     8041 gaacaccccg tctcggcaaa aaacggaaat cgtgctgctg actgtcatga aaacgtctcc
     8101 ttctgctgaa tcaggtcgcc gggccgaccg gttgctcccc cggaggggcg aaagcccctt
     8161 ctgccgtcca cttcttcggg gtttctcccc gcagtggacc tttccgatgg ggcagcgact
     8221 gccacggcac ggggcagttg tccgggggca gacgcaaaag tttttgacgc ctgcggcaaa
     8281 aagttttgtg gctggtacat ctctcccctg gacggctgaa acgtgttgtg gccgcttcag
     8341 cccccggaaa gtccacagcg ggaaacaggc cgcaggggtg gcggcggaag ggttgactgc
     8401 gccatcaggc gcagtggcct tacacaccgt caggtgtgca gggagcgccg tttccggcgc
     8461 gacaggggcg gcgcgaccgg aacggtcgct gcaggcctgg tttttcagac atcggaccgt
     8521 agcggcagcc cgaaaccgcg ccagcgggtt cggcggagac tggcgggtgc gccagcagct
     8581 gcctgtcgga gtcgggccga cgaccggatg ccgccaggca gccgggagcc gaagggtgtc
     8641 cccgcctccg ggcatcagaa caacagaaac gacaaaacag aaaaatgcct cacaccatga
     8701 cgggcaggaa gggcagcgcc gcaggcgcgg ccctcctgta tttcaggcag gcgtaagcct
     8761 gccagggttt tccggcggcc cggcaggcca tacccccgcc ggagcggggg agtaaatcac
     8821 cgacggaaca catgaccgtc aataaaggtg aaggagtcca ggaataaatc ccgcgcatac
     8881 gcctcatagt caaaatagag cgccaccgtc tccggcacgt cagccagcag gccggtatca
     8941 ctgacgaact caaccgcaaa agcctcctca ctgtcatcct cgccccacca ggcatcgcgg
     9001 aagctgtcaa aatccgtctc accggtatca tccacccaga gacgataagc ctcttcgcag
     9061 ccttcatccc gtgcccggcg gaatccttca acccaggccc agttgatatg acattcagag
     9121 gccatattcc ccgggaatcc ctcataatcc tgaaacatca gttccggatc ggtttcatcc
     9181 tggtgaaggg cacggcaggc ggcgaaaaaa tcgcgttcat catcaaacgt ggtcaggtca
     9241 aaccagcggc cggcgatgct tccgcagtta tatttgtgcc aggttccaac gtatacagca
     9301 ggtgcaacaa cagacattcc cgaatcctcc atagtcacat gttctgcgac accgggcggt
     9361 gccgtgcctc catctttgcc gggcctgcag ggatgcaagg gcgcaaggga agccgcagcc
     9421 tgacgcacac ggctggcggg agttggaggg tgagaccgcg aaatctgagc gggtgaacga
     9481 ccggaaaatc acggcagacg ggaaggaggg accgggtgac cgcagcggtt caccttacct
     9541 tgcaacccgc ccgcagaagc ccggctacga tggaggcacg gcacagcccg cacacgacgc
     9601 tgaggccaca gagctgaaaa cccacaaggc catgccccgc atggccgggt aaaagcggag
     9661 ccggcggcga cgtccttatt ccggcgagtg cagaggacac gccgcatgat ctccagacca
     9721 cccctggaac gaaacgatca tgaccggcat gtccctgctc ctcaagcata cctccactgg
     9781 ttcaccgaag aacatccgtg atgctcacaa gttttctgtc gcgcagccag tcctgcgctt
     9841 cacgcaccac acaactgtcc ggtttcaccg ctttcagcga tgatgccagt cgcttgatca
     9901 acatcaccag ttcccgtgtc tgtatgtccg gcattgtctc cggctgagca gggggactct
     9961 catttccgac gctcatccca tgcgcttcca gtgtggcacg gacaaaccag gcggcatcga
    10021 ccagttgctg tcgaatccag gccagatcgg cattcctgtc acgggctgcc attcgctcac
    10081 ccgacagcgc cctgctgata tggctgcgca ccagattatc caggagttcc tgcgcttctt
    10141 ccggtgaaaa gccacctggt atctctgtgc gcaacagccc tttcctgtac agaagctgca
    10201 gcagatcaac ctgcgaccgc acagcaacta tcagcacata acggacaaca ttcgcactgg
    10261 cagtggtttc tgtatcattc agcagttctt ccggatcgtt ctccccggtg ttgcagagca
    10321 acggcaacag aggtcgcatc cacgcgggaa cctggtattt ttcgtctgat ttcatcatat
    10381 tacgttctcc agcttgttcg ggatcatcca ctgctcaagt gcacgtacct gtaccggtgc
    10441 accacgaccg gcctcaccgg cttcaaaaat caaccgccag agtggttctc tcctgccaga
    10501 aaaaccggtc aaatgatgac agtgatggaa aacgcccgca ccacccccag aaaatcgttt
    10561 ccagatcatg ctcctcaaca acaccagacc agcgggacaa ccagcttccg accgtggcgg
    10621 gagtatgaaa cgcaaggtca atctgcgcct gagcaaaaca tgtctggtat tcccgttccc
    10681 gtcgttctgc ctcacgttcc cgctggcggc tgtatttctg ctctgtcagc gtggttttct
    10741 gacgacgccg ctcaccttca cgaaaaggaa cggacgggaa caaacgatgc cccgcatcgg
    10801 atggcagggg aagaacacag acttcaccaa aactttcaat cagtttgtcc actgcagcca
    10861 gccacaggag acggttgcca tgaaattcct ccttcgtcag cgcaggggca aaaaagcgga
    10921 gatcggtcag cgaaatccgg cgactgccat tcaggcagcg aaagaaagcg gtggcataag
    10981 gccatgctga aagtgtgcgt ccctgacgac gacgttgttc ggtgacagcc acagcctgca
    11041 tggcggcctg ttgttctgcg ctgaggggga tcagtgcccg tgaacgtgcg ctcatgcctg
    11101 acctccttct gccatcaaaa tatgaatgat gtcgttaaca ctgtatccct gcgcatcaag
    11161 tgtcgcggcg agcgccagcg tgtgactgat cgcgacaggc gtgaagatat gttgcacctg
    11221 aacgacggcg aaaggctgtc caccaccatt caccagaacc agcatccaga gctgagagac
    11281 gtctcccggc gaacataatg ccaggtggaa acgttcgtgg gctggcgtga aacgaacctg
    11341 gacggggaaa aagcccccga actcactgcc atactcgcca ttcatcatcc agttagccgg
    11401 tgcgtccact tcacgcatca cctcgtgagt gagattacgg cgcatatcgc ttcccgcagc
    11461 ccggatatct tcatattcct gtgcgctcat ggtctgtaat acattcaggg tcagttcagt
    11521 tttcatcacg gtatctccct tgctgccgcc ccgtctccgg cggggcggtg tgattgttca
    11581 tcaggcggca tcagccaggt tagcggtgtg ttcagtggca tctgtctgtg gacgtggtgg
    11641 acacatccag ccaggcaccc agcggttgtc tttcatgtag aactctgcat gttcagccgc
    11701 atcgcctttc ttcatcttct ccgcgtcccg tgcggcaccg gacagtccgg cctcattcag
    11761 ggctgcgata atctgcggct ttttcaggtg tccgaagaag tttgcttttg tcggctgcca
    11821 ccagtcgcgc atgtggaatc cgatagcgat ttccagcgag tcaagcggac tgcgtgacgt
    11881 gtgaccacac tcacgggtct ggacaccgtt aaggctgcat gcggtgcaga aactgagcag
    11941 ggataacagc acctcctgac tgagggacag gaatgtcgtc atgtcccgtg accatccctc
    12001 cggcagcaga gctgcaagac gggctttttc tgccatcatc gccatgaacg cggcaccttc
    12061 cttccccgat ggcgcatcgc tggtcagcga ataatgttta cattccaggc tgatttgtgc
    12121 tggtttactg tacgctccgc tgccaaacac attcaggcag agcgtccatg ccagcagtgc
    12181 cagggatttg tccggctgct gcatcagtgc cgcctggact gccagcgtgc gttcggaaga
    12241 cattttcgtc agcagcggca gactgattcc ctccacggag gctttctcct gcacctgttc
    12301 cgtgcggcca gcgtcgtcag ccacgtcatc ctcactgcgc aactgcacac cacgctggac
    12361 acatacgttt ccataacgcc aggacaccac cacgccactt ccggcacgca tctccggcgt
    12421 ccacgccctg accttcgcca tgcagtcaat cagcttcatt tctgcttcca gcaggtcgga
    12481 ttcctcacac tgattttcca gcgcgtcgta acgtgccatc agttcgttca ggcgttcttc
    12541 ttccgcctcc gtcagcacag cttccggctc cggcagactg cggtatgttc cggcatcctc
    12601 acggcagaaa ccgacaggct ccatacgtcc ggcgcaccat tcccagcctt cggcttcccg
    12661 aaggtgttca gcaacagcct ggagtttttc cagcagggcg gcatcgagcg ccacgcagtc
    12721 cacatagcca ccctcgtcgt cgctgaacaa atcggtgcgc agttcatctg gcgagaaggc
    12781 atcagccccc acgaagcgga atttactgtt ccccgccacc gccacttcac tttcggtaat
    12841 caggttgcgg ataacccgca catccggttt accgccccat cctgactgac aggcggcttc
    12901 aaacacctgc acctgacgcg cggtgtcgtt ctccagcgcc agcgcctgac agtgctctgt
    12961 ggtgatgcgg tcctctgcca gcgcatcgag aatgacaggc gcaaggtctg ccagtttcag
    13021 cattcgctga acgtggcggg gtgaatagcc cagcaaatca ccgatttgtg caggtgtttt
    13081 gccttcctgc gccattgcac ggaatccggc aatctgttcg gcagggtgca tatcccgacg
    13141 atgaccgttt tcagtcattg atgcggcagt cgccagttcc tgcggaataa ttttcacgcg
    13201 gacaggccag tcagccggaa tgattccacg ctctgccagc atgttgagtg ctgccagcct
    13261 gcgaccacct gcggcgacac cgtggcggtc acctggcagg gcatgaacaa ccagattctg
    13321 cagcaggccg acccccttaa tggaatcagc cagttcactg acggactccg cagaatacgg
    13381 caccgtgcgc acattcagcg gtgacttaat cagtgaggcc agcggcacgc tcacttcctc
    13441 cgtctgcgcc agcagggcag acaggactgt ttcctgcgtt tttgcaggtt tacggctgga
    13501 tttacgctcc gtttttgtct tagactctgt aactgacata tatttctcct ttgaccggga
    13561 tggttgtatg tctgccggac gcgttcccgt ccggcacctc tcttacttaa gaatgccgaa
    13621 atcccggatg acatggttca tccagtaccc ggcaccgtcc tcaaaattcc aggtacgcca
    13681 gaccattgtt ccgttattac ggacaaccag gcgaaaatgt tctccgtggt catcctcgat
    13741 aaagacgttc cggtactgtg cggcaacggc ttcggcctgt ttgcgggtaa aaggaccgtc
    13801 aggcagtccc tgaagtattc tgaaatattc gctcatcctc atatctccgg tgatgccccg
    13861 cgcagggcgg ggcggttatc acagtcaggc cagtcagaac gggatatcgt ctgaaaaccc
    13921 gtaatcctcc ccctccggcg gttgcggctg agactcgggc tgcgcggcct tacgtccacg
    13981 gcctttcgtt ttcgcgccac cttttttcgt cgcgtcagca ctctgtggct gcccattctg
    14041 ctgaggcttc ggttgcgcct gagcgttctg ctgtggtgca ctccccagca tctgcatggt
    14101 gcccgtggtc ttaacaagaa tttcagtgac gtaacgggtg ataccgttat cttcccagct
    14161 acgggtacgg agttgcccct cgatgtagac ctgcgcgccc ttgcgcagat attcacctgc
    14221 cacttccgcg agcttgccga acagcaccac gcggtgccat tccgtctgct cccgcatctc
    14281 ccccgtctgt ttgtcgcgcc agctttctga cgtagccacc tgcaggtttg ccactgcgcc
    14341 cccgttgggg atgtaacgga cttccggatc attgcccaga cgcccgacga ggatgacctt
    14401 gttgatacca cgtgcactca taagaacctc ctgactgaaa gatgtgagat aaaccggata
    14461 ccggcgacgg gacgttcatg tgccccttca gaggagcgaa agctcctcct gccgtccact
    14521 tcacaggggt ttctccccgc agtggacctt tccgatgggg gcccgtttac cgcctcacgg
    14581 ggcagctgtc cgggggcgga cgcaaaattt tttgccgggt tcatggctaa aaattttgtg
    14641 gccggtgcat ctcaccctgg acagatggaa cgggaggggg ttacgtctgc cccccggaaa
    14701 gtccacagcg ggaaacaggc cgcaggggtg gcggcaggag ggaggactgc tgcgccatca
    14761 ggcgcagttt cctcacacac cgtcaggtgt gcagggagcg caggtcacgg cgcgacaggg
    14821 gcggcgcgac cggaacggtc gctgcaagcc cgggttttca cgcatcggac cgtagcgaca
    14881 gcccgaaacc gcgccagcgg gttcggcgga gactggcggg tgcgccagca gctgcctgtc
    14941 ggagtcgggc ctgacgaccg gatgccgtca ggcagccggg agccgaagtg ggcgcaacaa
    15001 aaaacagcca gaaacggtga cagtgtcacg gaacatcaca cctgttcctg gcatacagcc
    15061 atacactaca ttctgaccgg aaagccgggc cggcagcgcc gcaggcgctg caccccgcgt
    15121 ttccggcagg cgccagcctg ccaggggttt tcgcgcgggc tgacaggcgc cctccaccgc
    15181 ctgcccctgt atcacctgtt tctgctgccc tgtaagatgt agtgaccgcc caggaaaacc
    15241 gcctgctgat tcgccacaaa atcgcagatt tcatcaacaa tctgcgcggt ggtgagcggt
    15301 ttctgcccca tttcacgacg ccgcgcatta atcacggccc tgtaattacg cagaatttcg
    15361 cgggtttctg tgctgatatt catgacatcg tctccccgtc cagcgcctgt aacacaacgt
    15421 ccattgcttc gcgttcctcc ctgacgaacg catcaccgtc gcccccgttc tcactcccga
    15481 cggcatacag gcgcttcatt tcactgcctg ccagcgccat cactttttcc agcatcaccg
    15541 ccgggactgt cacatgacgg ctggcgcgct tttcttccat cagttcacga acaacgtcgt
    15601 gaacaacgct gacatccgca ccatgctcaa aggcatcgtc cagtcgctgc atgacggttt
    15661 ccagctcatc gtctgacagg ttccagtccc gggctatgtg acgcacatcc ccacgggtca
    15721 gcaccacggc aaaaacgcat tcactgtccg gcacgtctgt atccagtaca tcgcggatga
    15781 tttctttaac agtacagtac atgttctcgt ctcctttaca ggggcggcat tgccgccccg
    15841 tccgttaatc agtcaagtgc cgcgtaaatc gcgttgcatt cggggtgaaa ttcgatgtgg
    15901 ctccacaact gcgcatcacg catcctgtac aggtgtgtca accctgcatc cccgctgtcg
    15961 tggtacagcc acaactggcg gttaatcaca agggaggtaa ggatgatgcc tgcggcatca
    16021 gcactcacgg tacggtcaaa ccagtttgca ccgttcacca tgtggaaacg gtcgccatcc
    16081 ggcatcatgt aaccgccacc gtccggcagg cgaatgaagt gccagaatcc gccttcatat
    16141 tccggcatca tttttttcgc cagcgcgtat acagacgctt cggcatacag aaaatccagg
    16201 ccaaacaggt aaggcagaaa agacagacgg tcgcactctt caacgttcag agtgacaggt
    16261 ttcgcatact gcatgggatt tcctccgttg ttcacatatt ccgcgacacc gggcggtgcc
    16321 gtgcctccat ctttgccggg cctgcagggt tgcaagggcg caagggaagc cgcagcctga
    16381 cgcacacggc tggcgggagt tggagggtga gaccgcgaaa tctgagcggg tgaacgaccg
    16441 gaaaatcacg gcagacggga aggagggacc gggtgaccgc agcggttcac cttaccttgc
    16501 aacccgcccg cagaagcccg gctacgatgg aggcacggca cagcccgcac acgacgctga
    16561 ggccacataa ctgaaaaccc acaaggccat gccccgcatg gccgggtaaa gcggagcctg
    16621 cggcgacatc cttttgctct ttccggggac cggcaggaaa aaggcgggga tttaccccgc
    16681 cagtgtcatc atttacggat ttttgccagg gcatccgcca tgctgcggat ttcggtccgg
    16741 gcttcccgga ccacctcccg cagaacacca cggtcaaacc agcggcgggg cggttcatcc
    16801 ggtatacgca gcacatcgca ggccagccca ctggacagga tatgctcaat acaatcgctg
    16861 accaggtaca tttccactgc cgaaaaacgt gcgccccggt tcacgatggc atcgagcact
    16921 acgggcaggt tttcgtcatc ttcatcgagc cagcagggat gatcgctttc cttcacgacc
    16981 aggcgaaagc ggcgcgtctg gcgggtcagc aacgtctgac cggtggtggt cagcgccgca
    17041 cggatacgcc gcgtatcttc tgccacctgg taacagatgc ttcgtaccgc atcgtgcacc
    17101 gcatcaagaa cagccctgat ttcgtcttcg gtcagccatt ctccggcata cgggaaagac
    17161 acctgcgttc cgtctgccgg aagtaccggc agcgactgcc actgactgcc ggacagtacc
    17221 agttccgccc cggttccggt cagtctctga atggcagagg ccaggagcgg cggcagtacg
    17281 ccgccgtgac ggttgataag taacgtcgcc agccggggcg catccagccg cacgtaaatg
    17341 ctgatccagc ccggatagtt cggatttcgc tggtcaacat ccgtctcttc cggccagccc
    17401 tgcgccagca gcaggttacg ggcgtgacga caaaccagtg cattcagcgt tttgttcatg
    17461 attaagcgtt ctccctgtat cgccgcccgc agggcaggcg gcgataacat cagttgtttt
    17521 cgtgtgtgtt gtttttcagc agggtgcggg cctgattaat cactgcctga cggcgggtaa
    17581 cacattccgg gacggcaagg ccgttatcac gggcggccca ttcacgtttc cacagtgcct
    17641 gttcggcggt ctgaatcagg gacgccagca gttcagccgg gacggtcacc tggcggtttt
    17701 cgctcacgtt gctgataatc tccatcgcag cggcagaaga aataccggat tcactccgct
    17761 ggtcttccgg gatgtcctcc aggcgcgaca ggacggtcct gatttcatga tcggtcagag
    17821 aaatttccat accgtcagcc agtgcctgaa tctcttccgg ggaccagaca accagcatca
    17881 gcggcgtgtc tcccggatac tgcgctgcca gctcccggca cagcgtttcg catgttccgt
    17941 acatttcgtc tcccccttaa tctttgaagc acgcatccag ttcaagaatt tcggtatacg
    18001 cctccataaa caggcgattg gcgcgataca gcgtctggcg tgtcaggcgt tcaacaagca
    18061 catcggaatc aaaacgacag cgatgctcca tatcgttcag cctggcccga aggtcacaca
    18121 tggtgttggc cacctccgcc acttcggcgc gaagtttttc gtgtccggca tagttcattt
    18181 acgcagcctc cggcataaac cagtcatcat tcgcggcccc ctgctgcatg gcccgttgta
    18241 cggcagccag tcgttgctgc ccggcacggt gatactgctc aagcagctcg ataccgatat
    18301 accggcgtcc ggactggagg gcggcaacgc aggttgagcc gctgcctgca aacgggtcca
    18361 gcacaattgc gttcgggtgt gtgaagctct caatcagcgg ttgcaggctg gtaacgggct
    18421 tttccgtcgg gtgatggcga ttgcccgaat atttccagcc cagcacgtcc ggcagcgggt
    18481 tttgtggcag acgtggacgg ccttttgcca ggatgtaggc gcattcgtgg cgatagccca
    18541 catatgcggc cttcgatgtg tagtttttgg tgaagaccag gtgaccaaca acgctgaatc
    18601 ccgcattttt ccaggcggcc ataaagcgat cgacgcggtt ccagccatag aagctgacca
    18661 tcagcgcgtc ttttttgagt acgcggtaca tttcattaca ggccggttgc agccattcat
    18721 cggttttatc gccggcgatg gtgcgcccgg aacggtcacg aaaaccgacg aggtacggcg
    18781 ggtcagtcag tatgaaatca atggcgttat caggaatacg ggccataaca tcgatgcagt
    18841 taccgaggac aaaacgggac ataacaacgc tccttctctt acagaggaca ccgggagggc
    18901 actcctgtgg tgcccgttcc ccgctgcctg tgggctgttc accgcgtccg gtaacgggtc
    18961 aggccgaccg tggaataccg cagccgtcgt ctttctgacg gcaagg
//
LOCUS       DBBGLQ010000083        19005 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.083, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000083 DBBGLQ010000000
VERSION     DBBGLQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.083"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            203..1060
                     /gene="focA"
                     /locus_tag="ACIYSB_003847"
     CDS             203..1060
                     /gene="focA"
                     /locus_tag="ACIYSB_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGC5430794.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1115..3397
                     /gene="pflB"
                     /locus_tag="ACIYSB_003848"
     CDS             1115..3397
                     /gene="pflB"
                     /locus_tag="ACIYSB_003848"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGC5430795.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3589..4329
                     /gene="pflA"
                     /locus_tag="ACIYSB_003849"
     CDS             3589..4329
                     /gene="pflA"
                     /locus_tag="ACIYSB_003849"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGC5430796.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4411..5001)
                     /gene="ycaK"
                     /locus_tag="ACIYSB_003850"
     CDS             complement(4411..5001)
                     /gene="ycaK"
                     /locus_tag="ACIYSB_003850"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HGC5430797.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            5101..6009
                     /gene="ycaN"
                     /locus_tag="ACIYSB_003851"
     CDS             5101..6009
                     /gene="ycaN"
                     /locus_tag="ACIYSB_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5430798.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMSLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(6010..7440)
                     /gene="ycaM"
                     /locus_tag="ACIYSB_003852"
     CDS             complement(6010..7440)
                     /gene="ycaM"
                     /locus_tag="ACIYSB_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGC5430799.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7650..8798)
                     /gene="ycaD"
                     /locus_tag="ACIYSB_003853"
     CDS             complement(7650..8798)
                     /gene="ycaD"
                     /locus_tag="ACIYSB_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5430800.1"
                     /translation="MSTYTQPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9112..9738
                     /gene="ycaC"
                     /locus_tag="ACIYSB_003854"
     CDS             9112..9738
                     /gene="ycaC"
                     /locus_tag="ACIYSB_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGC5430801.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRLSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9773..10636)
                     /gene="dmsC"
                     /locus_tag="ACIYSB_003855"
     CDS             complement(9773..10636)
                     /gene="dmsC"
                     /locus_tag="ACIYSB_003855"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGC5430802.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10638..11255)
                     /gene="dmsB"
                     /locus_tag="ACIYSB_003856"
     CDS             complement(10638..11255)
                     /gene="dmsB"
                     /locus_tag="ACIYSB_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGC5430803.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11266..13710)
                     /gene="dmsA"
                     /locus_tag="ACIYSB_003857"
     CDS             complement(11266..13710)
                     /gene="dmsA"
                     /locus_tag="ACIYSB_003857"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGC5430804.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(14007..15002)
                     /locus_tag="ACIYSB_003858"
     CDS             complement(14007..15002)
                     /locus_tag="ACIYSB_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5430805.1"
                     /translation="MSIKQLKDGRYQVDVRPQGAGGRRIRKIFTLKSKAQEFERYVLQ
                     NFHNNPWQARPADQRRLSELIEVWWMLDGRNQAYGDTYRTRLEKVIREMGDPRASQMT
                     RKFMIEYRSDKLQAGLMPSSINRDLCALSAMFSLLIDAEVYHNENPVRGIRKLKVQNT
                     EMAFLSDDEIDRLLKRLEGDARRIAILCLSTGARWKEASTLRGEHIVGNRVTFFNTKN
                     GKSRSVPVADSVVPLIKTRRTGLLYQVDYLSFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLAESVHKLSIDQ"
     gene            complement(15072..15413)
                     /locus_tag="ACIYSB_003859"
     CDS             complement(15072..15413)
                     /locus_tag="ACIYSB_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5430806.1"
                     /translation="MSILSIDYAKKLRLIRKTEGLTQKQFADITGLSLATVRNYESGQ
                     KIARAKIVEAVLQVDRFEKYMFWLIKDKTLPVAGQIAPALSLDGSTQSEDDQVSTRNT
                     QKSPQSGRNAG"
     gene            15517..16038
                     /locus_tag="ACIYSB_003860"
     CDS             15517..16038
                     /locus_tag="ACIYSB_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HGC5430807.1"
                     /translation="MREIIADKELSGAQPVQDDGADNEFIEAANDNEHLETKTRPSYK
                     KGATLRLDGPVAGLVSLEKGAAYLGVTKSALRAAIQRGQMPGHKTRSNPEDEKSDGTW
                     WFNAKAWDELADELLECEPPEWHNWKSYWTYDRKKRKFSPANKEDCQTVKGKQVYSPR
                     VSKLEQLKNNQPG"
     gene            16047..16253
                     /locus_tag="ACIYSB_003861"
     CDS             16047..16253
                     /locus_tag="ACIYSB_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430808.1"
                     /translation="MTSNIISAILTLVFIEIGVIAIYLFRKLTGHEERFIELSIEYIA
                     AYTKGLFPAAIGAMFIAFVIWFIG"
     gene            16505..16843
                     /locus_tag="ACIYSB_003862"
     CDS             16505..16843
                     /locus_tag="ACIYSB_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HGC5430809.1"
                     /translation="MKQQRNSRFRNGAERHANRFATSASRSNSRYSLSETHATPGGHA
                     VKQTGQHTWLIEQAGLVIHRCHRNPFTGNRIFALSSGDNQFGQDFTLHEALRTADRLI
                     EGRSFQHTDY"
     gene            16882..17124
                     /locus_tag="ACIYSB_003863"
     CDS             16882..17124
                     /locus_tag="ACIYSB_003863"
                     /inference="COORDINATES: protein motif:HMM:NF027276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HGC5430810.1"
                     /translation="MKRAYKELINHALQNYHYGLTSRGTNGDAVSLAALTTAIKGLTC
                     CAFGSGDNEALTELQAINKDLSEGNVITPFVIQEEA"
     gene            17121..17246
                     /locus_tag="ACIYSB_003864"
     CDS             17121..17246
                     /locus_tag="ACIYSB_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430811.1"
                     /translation="MSVFMSWLILILAIICAVGIMRVINSINTIESLFTGDKRRK"
     gene            17327..17620
                     /locus_tag="ACIYSB_003865"
     CDS             17327..17620
                     /locus_tag="ACIYSB_003865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430812.1"
                     /translation="MLNNKKNRIIDNSEQVNKIIEGTNKLIKKKNKLSRELMEHPEEI
                     LLAMMHQTEAIAIALAVQALSEFREENIRLAQELKNLRRQLQSTEAGTSSRMH"
     gene            17631..17840
                     /locus_tag="ACIYSB_003866"
     CDS             17631..17840
                     /locus_tag="ACIYSB_003866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430813.1"
                     /translation="MTAILRRRNKTLFTDTSGMEYEVESSVIATTTRCPAGDELIYVH
                     LTDGSQITVLTESWRELEIISEVRT"
     gene            17837..18049
                     /locus_tag="ACIYSB_003867"
     CDS             17837..18049
                     /locus_tag="ACIYSB_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430814.1"
                     /translation="MSSKHDTNARRPGKEARHHIAVMRNLLMSEIDDFVAGLGQPGEP
                     ATPGQIHRELRRRVENVVDYVINPND"
     gene            complement(18089..18571)
                     /locus_tag="ACIYSB_003868"
     CDS             complement(18089..18571)
                     /locus_tag="ACIYSB_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430815.1"
                     /translation="MQNNQRKKSRERDEVMFRLWYSYWLERIAAVFNRRLDAIINILI
                     LAAGAFIVAGWHFGWVLGAVVVVLGACNFICRFGQRAQAASEHAHRYNELITKSALLS
                     TEALLARLPELEMHDSPVLECMENVAYNKACMSLNISHRESLSFAEKMMAAITIGLSR
                     "
     gene            18806..>19005
                     /locus_tag="ACIYSB_003869"
     CDS             18806..>19005
                     /locus_tag="ACIYSB_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGC5430816.1"
                     /translation="MPALKPMGVSGVPPKHVRAWTQQEDALLVGLYPSHTVPELTKHF
                     QRTGPAIRSRIRLLLSQGVIHT"
BASE COUNT         5146 a         4889 c         4441 g         4529 t
ORIGIN      
        1 cggaggatgc ggaaaaaatt caactcattt gttaattttt aaaatttatt tttatttgga
       61 taatcaaata tttactccgt atttgcataa aaaccatgcg agttacgggc ctataagcca
      121 ggcgagatat gatctatatc aatttctcat ctataatgct ttgttagtat ctcgtcgccg
      181 acttaataaa gagagagtta gtgtgaaagc tgacaaccct tttgatcttt tacttcctgc
      241 tgcaatggcc aaagtggccg aagaggcggg tgtctataaa gcaacgaaac atccgcttaa
      301 gactttctat ctggcgatta ccgccggtgt tttcatctca atcgcattcg tcttctatat
      361 cacagcaacc actggcacag gcacaatgcc cttcggcatg gcaaaactgg ttggcggcat
      421 ttgcttctct ctggggctga ttctttgtgt tgtctgcgga gccgatctct ttacttccac
      481 cgtgttgatt gttgttgcta aggcgagtgg gcgcatcacc tggggtcagt tggcgaaaaa
      541 ctggctaaat gtctattttg gcaacctggt cggcgcactg ctgtttgtac ttttaatgtg
      601 gctttccggc gagtatatga ccgcaaatgg tcaatgggga ctaaacgtcc tacaaaccgc
      661 cgaccacaaa gtgcaccata cttttattga ggccgtctgt cttggtatcc tggcaaacct
      721 gatggtatgt ctggcagtat ggatgagtta ttctggccgc agcctgatgg acaaagcgtt
      781 cattatggtg ctgccggtcg cgatgtttgt tgccagcggt tttgagcaca gtatcgcaaa
      841 catgtttatg atcccgatgg gtattgtaat ccgcgacttc gcatccccgg aattttggac
      901 cgcagtcggt tctgcaccgg aaaatttttc tcacctgacc gtgatgaatt tcatcactga
      961 taacctgatt ccggttacga tcggcaacat tatcggtggt ggtttgttgg ttgggttgac
     1021 atactgggtc atttacctgc gtgaaaacga ccaccattaa tggttgtcga agtacgcagt
     1081 aaataaaaaa tccacttaag aaggtaggtg ttacatgtcc gagcttaatg aaaagttagc
     1141 cacagcctgg gaaggtttta ccaaaggtga ctggcagaat gaagtaaacg tccgtgactt
     1201 cattcagaaa aactacactc cgtacgaggg tgacgagtcc ttcctggctg gcgctactga
     1261 agcgaccacc accctgtggg acaaagtaat ggaaggcgtt aaactggaaa accgcactca
     1321 cgcgccagtt gactttgaca ccgctgttgc ttccaccatc acctctcacg acgctggcta
     1381 catcaacaag cagcttgaga aaatcgttgg tctgcagact gaagctccgc tgaaacgtgc
     1441 tcttatcccg ttcggtggta tcaaaatgat cgaaggttcc tgcaaagcgt acaaccgcga
     1501 actggatccg atgatcaaaa aaatcttcac tgaataccgt aaaactcaca accagggcgt
     1561 gttcgacgtt tacactccgg acatcctgcg ttgccgtaaa tctggtgttc tgaccggtct
     1621 gccagatgca tatggccgtg gccgtatcat cggtgactac cgtcgcgttg cgctgtacgg
     1681 tatcgactac ctgatgaaag acaaactggc acagttcact tctctgcagg ctgatctgga
     1741 aaacggcgta aacctggaac agactatccg tctgcgcgaa gaaatcgctg aacagcaccg
     1801 cgctctgggt cagatgaaag aaatggctgc gaaatacggc tacgacatct ctggtccggc
     1861 taccaacgct caggaagcta tccagtggac ttacttcggc tacctggctg ctgttaagtc
     1921 tcagaacggt gctgcaatgt ccttcggtcg tacctccacc ttcctggatg tgtacatcga
     1981 acgtgacctg aaagctggca agatcaccga acaagaagcg caggaaatgg ttgaccacct
     2041 ggtcatgaaa ctgcgtatgg ttcgcttcct gcgtactccg gaatacgatg aactgttctc
     2101 tggcgacccg atctgggcaa ccgaatctat cggtggtatg ggcctcgacg gtcgtaccct
     2161 ggttaccaaa aacagcttcc gtttcctgaa caccctgtac accatgggtc cgtctccgga
     2221 accgaacatg accattctgt ggtctgaaaa actgccgctg aacttcaaga aattcgccgc
     2281 taaagtgtcc atcgacacct cttctctgca gtatgagaac gatgacctga tgcgtccgga
     2341 cttcaacaac gatgactacg ctattgcttg ctgcgtaagc ccgatgatcg ttggtaaaca
     2401 aatgcagttc ttcggtgcgc gtgcaaacct ggcgaaaacc atgctgtacg caatcaacgg
     2461 cggcgttgac gaaaaactga aaatgcaggt tggtccgaag tctgaaccga tcaaaggcga
     2521 tgtcctgaac tatgatgaag tgatggagcg catggatcac ttcatggact ggctggctaa
     2581 acagtacatc actgcactga acatcatcca ctacatgcac gacaagtaca gctacgaagc
     2641 ctctctgatg gcgctgcacg accgtgacgt tatccgcacc atggcgtgtg gtatcgctgg
     2701 tctgtccgtt gctgctgact ccctgtctgc aatcaaatat gcgaaagtta aaccgattcg
     2761 tgacgaagac ggtctggcta tcgacttcga aatcgaaggc gaatacccgc agtttggtaa
     2821 caatgatccg cgtgtagatg acctggctgt tgacctggta gaacgtttca tgaagaaaat
     2881 tcagaaactg cacacctacc gtgacgctat cccgactcag tctgttctga ccatcacttc
     2941 taacgttgtg tatggtaaga aaacgggtaa caccccagac ggtcgtcgtg ctggcgcgcc
     3001 gttcggaccg ggtgctaacc cgatgcacgg tcgtgaccag aaaggtgcag tagcctctct
     3061 gacttccgtt gctaaactgc cgtttgctta cgctaaagat ggtatctcct acaccttctc
     3121 tatcgttccg aacgcactgg gtaaagacga cgaagttcgt aagaccaacc tggctggtct
     3181 gatggatggt tacttccacc acgaagcatc catcgaaggt ggtcagcacc tgaacgttaa
     3241 cgtgatgaac cgtgaaatgc tgctcgacgc gatggaaaac ccggaaaaat atccgcagct
     3301 gaccatccgt gtatctggct acgcagtacg tttcaactcg ctgactaaag aacagcagca
     3361 ggacgttatt actcgtacct tcactcaatc tatgtaatta gatttgactg aaatcgtaca
     3421 gtaaaaagcg tacaataaag gctccacgaa agtggggcct tttttagcgc gagagccttt
     3481 tttgtcagct atctatactt taaggtgact gccaaaacag actcgacgta gccttcgagc
     3541 tgcgcaccaa cacggcctca gatgggccac atctggagaa acaccgcaat gtcagttatt
     3601 ggtcgcattc actcctttga atcctgtgga accgtagacg gcccaggtat tcgctttatc
     3661 acctttttcc agggctgcct gatgcgctgc ctgtattgtc ataaccgcga cacctgggac
     3721 acgcatggcg gtaaagaagt taccgttgaa gatttgatga aggaagtggt gacctatcgc
     3781 cactttatga acgcttccgg cggcggcgtt accgcatccg gcggtgaagc aatcctgcaa
     3841 gctgagtttg ttcgtgactg gttccgcgcc tgcaaaaaag aaggcattca tacctgtctg
     3901 gacaccaacg gttttgttcg tcgttacgat ccggtgattg atgaactgct ggaagtaacc
     3961 gacctggtaa tgctcgatct caaacagatg aacgacgaga tccaccaaaa tctggttgga
     4021 gtttccaacc accgcacgct ggagttcgct aaatatctgg cgaacaaaaa tgtgaaggtg
     4081 tggatccgct acgttgttgt cccaggctgg tctgacgatg acgattcagc gcatcgcctc
     4141 ggtgaattta cccgtgatat gggcaacgtt gagaaaatcg agcttctccc ctaccacgag
     4201 ctgggcaaac acaaatgggt ggcaatgggt gaagagtaca aactcgacgg tgttaaacca
     4261 ccgaagaaag agaccatgga acgcgtgaaa ggcattcttg agcagtacgg tcataaggta
     4321 atgttctaat ttccccgtaa agcggcaact ttattgagtt gccgcttttt tattccgcgc
     4381 ttcattctgc tacagcgttt ttaacacatc ttatagtgca tctaccatat cccgtacctg
     4441 agataataac gactgataat agctcgcgtg aagttcttcc ccatcgaata ccactgtatt
     4501 acacaagaaa gtgacatcgg catcttcaat accaagataa ctgcacatat tttttaaata
     4561 atcgcttata tttttttccc agcccatctg gacaaatgat tctttgtctc ctccaaccag
     4621 cgccacccaa cgaactttat tgaatggtaa tttgtgccca tctccataag ccagcccatt
     4681 attccatact ctgtcaatat atccttttag cattgccggg aagctgtacc accagagagg
     4741 aaaaaccacc actaacgtgt catgttcaag cagctctgaa taaagctgat gaacctctgg
     4801 agaataacgt ttatccatat tcttccagtc tggttcatct tccggcgtca tcactgggtt
     4861 gaaattacgc cgatataaat cgagttccgt cacctgtatt ttccgctcca ttgcccgctg
     4921 atggatcgca tcagcaatat gtgcggtcaa tgaatcatga cgaggatggg cccataccaa
     4981 ataaatacgt tcagactgca taattttacc tccacgttaa gttctggagc gcactttacg
     5041 caggcggcat aatcctgtaa acgtgatgat tcaggatgag ttaatgaagg attttcacta
     5101 atgcggatga atatgtctga ctttgccact ttctttgccg tggcccgtaa tcaaagcttt
     5161 cgtgcagcgg gcgatgagtt aggcttatcc tcgtccgcca ttagccatag tattaaaaca
     5221 ctggaacaac gtcttaaaat tcgcttattc aatcggacaa cccgaagcgt atcgttaacg
     5281 gaagcgggtt ctaatctcta tgaacgattg cgcccagctt tcgacgaaat tcagatcatg
     5341 ttagatgaga tgaatgattt tcgtctgaca ccgacaggaa cgctcaaaat caatgccgcc
     5401 cgggtagctg cacgtatatt tttaatgtct ttactggttg gtttcacacg tgaatatcct
     5461 gatattaaag tcgagctcac aactgacgac tctcttgtcg atatcgtcca acagggcttt
     5521 gacgctggcg ttcgacttag ttgcatcgtc gaaaaagata tgatcagcgt ggcgatcggc
     5581 ccgccagtga aattatgtgt agcagcaacg ccagagtact ttgcaagata tggcaaaccc
     5641 cgccatcctc atgatctgct taatcatcaa tgtgttgttt ttcgttatcc cagcggcaaa
     5701 cctttccact ggcaatttgc aaaggagctg gaaattgctg tcgcaggaaa tatcattctg
     5761 gatgatgttg atgctgaact ggaggccgtt ttgatgggtg ctggtatcgg gtatctgctc
     5821 tatgaacaga tcaaggagta ccttgatact ggtcgtttag aatgtgtact ggaagactgg
     5881 agcacagagc gtccaggctt ccagatctat tatccaaacc gccagtatat gtcctgcggc
     5941 ttgcgcgcat ttctcgatta tgtaaaaacg ggtcagatct gccagagtca gcgtcaccga
     6001 ccacaataat tatttactat tcgctttacg cgccagcagc gggaatatca gtcctaatcc
     6061 taccagcaca aacggcgttg caacattcag cgccaactgg aaggtccact ctgcagtgaa
     6121 ggcttccatt ttcgggaaga tccccgtcaa acaggcaaag gcggtaaagg caaaacacca
     6181 aatcccgacg gtcattgcca gaggcttatt acgaataaag acatattctg gtttatattt
     6241 ctgcgccaag cgaacgactg caataaatgc aacaaatacc cataaataac gcagcggcat
     6301 cactaccgaa ttaaggttca acagccattt atagagattg ttcatatcac caatgccgag
     6361 agtcggcagc ataatcagaa tcgccaccag taccagggtc agaaaatagc cattaacggg
     6421 cgtaccagaa gcgttggtac gacataaact ggctggaata tatttgctgt cagcatcacc
     6481 taatagcact ttaagcgggg catcaatcga gaataccagc gccgctactt gtcccagggt
     6541 attcgcaatg gcgtaaatca ccattaaagt attacccatg ttgtaatact cgcccagctt
     6601 ctgaaaggcg taatactgac cgttggtcat taagtcatcc gggatattac gcgaatcaaa
     6661 catcatcccc atcgccagcg agcccagaat ggcacaaacc gcaaccatca ccgccaggca
     6721 taacatccct tttggaaatt cttttcctgg gttgcgcgtt tgattaacgt aaggagaaat
     6781 cttctctgct ccgccaaccg cgaaaaccag cattgaaata gtggtaatgt aggtaaagtc
     6841 gatatgagga atgaacgttt cccaggtaat gtttgtggtc gcaatatgca cttcagtaat
     6901 tgcaggcgcg gttaccgcca tcgccacata caggagcgac ataacaaaca tagcaatccc
     6961 tgccacagaa ccgacgattt tcagcgattt cataccgcgt gaagcaaccc acataaagaa
     7021 gataaacagc accagcgtta acccctgtaa cgctacgact gaatattctt tgattagcga
     7081 accgtcgcct ttcatcgccc aaccgagcgc aatcagaatt gcctggggtt tttgtgccag
     7141 atagggaata tgcaccaccc agtaggtcca cgcggcgaga taagccagtc cgggtcccat
     7201 cgtgtgttta atccaggtac tgaccccgcc cttcccatct ttgaaggtcg agcctaactg
     7261 accaacaatt agcgcataag gtgtgaaata gagtgcaaag ataaacaccc atgaaaaaac
     7321 aaccaccagc ccctggttgg catagttatt aacaacgttg ccaaaacccc agacagtgat
     7381 aaaagacatc agcgcaatgt tgtaccatcg caactgtttt tcctgaacat tcccagccat
     7441 agacaaatcc ttcgccaaaa ttaaaggggc atctgtaaaa cgagggatta tgcgcggcaa
     7501 ataagaagtg cggaaatagc gaaatgcaaa attataacct gctgcacatt ttatttttaa
     7561 acgaataaaa tttggaataa agcaagtcag atagaagaaa caaaaaagca gagtgtgaaa
     7621 cagtaagttc acactctgct tgaattcatt tacacgtgag caacgggttt cggcgtatga
     7681 ccggcgttgc gcagcagcat cagcaaatag ataaacgata cgctggcgat catgataaac
     7741 aataaattat cggagaaatt ctgcattagc atagcggtaa atgacgggcc aagcagactt
     7801 cccacagtat agctcaacag taaggcctgg ttcatcgcca ccagttgatg atgttcaact
     7861 ttctcgcaag cccatgccat cgccaccgga tatagcgtaa agccagcggc accgaggatg
     7921 aataacgctg gggccatcgc cgcctggcta agcatcgcga tactgccgag aatgacgaca
     7981 aagacctgaa cacgcaacac cagcagtcga ccaaacttat ccgccagacg tccaatcggc
     8041 cattgtccaa ggatacccgc actgaccagt accgccatcc agaaaccaat gctggcattg
     8101 ctcacccctt tgtgattgag gtacagcggc atcaggccat atagagatcc cagaacgatt
     8161 cctgagataa tgcagccatt cacgccaagc cgcgcctgac ggagttttag cattgacgta
     8221 attgacgtcg aatcatggtt ttcagcctgc tgattcagca cacgcgtaaa caacagcggt
     8281 aagatccctg ccaacgtcaa acctgtaacc cacggcaata cggacatcag ctcggttgaa
     8341 actttgctga ccagtaactg gcctaaaaac gttcccacgt aataaaccat catatacgca
     8401 gcaagcaaac gcccacggtt acgtgacgtc ccactgcaca tcagcgcgct ctcaacaacc
     8461 acccaaatca tggcacagcc gacgcccgcg acaaaacgcc aagccaacca gctccagaat
     8521 ccaatcatca ggccaaggcc ggcacagcca gcggcaaaaa tgaaggaggc cagataatag
     8581 ctgcggttaa agccaatgcg cttaatgaca taccctgtca gcaatgtacc gacaaggttg
     8641 ccggtaaaat aggatgagct gacaacgcct acctgccatg tggacatgtg ttcctgggcg
     8701 agccaaagcg gcacgagtgt atttaacacc gcaatcgcca gagtcaacaa aagcaggcca
     8761 gacagcaaaa gcatgacagg ctgggtatac gtggacatga attaaaaccg tgaggaagtt
     8821 caaatttcat gcgcatcatg ccaccgccaa aaacattgtc aatgggtgaa aatacgcgat
     8881 atcccagcgg cggtattatc gatttatatt actcattcat aacgctaatg ctgaagagat
     8941 gattagtctc atctgattgt ttttattgaa ctaacacaaa tttcatacgc ttcgttcagt
     9001 cgaaaaattt catggtcaca gcaaactcat atttcccgtc tatgctttca gagcaatgca
     9061 tcgcgtctga tcgatttcag acgtaccact gactctcgaa aggagaagag gatgaccaaa
     9121 ccgtatgttc gtcttgataa aaatgatgct gccgttttgc ttgttgatca ccaggctggt
     9181 ttactttccc ttgtacggga tatcgaaccc gataagttta aaaataacgt gctggcgctg
     9241 ggtgacctgg ctaagtattt caacttacca actattctta ccaccagttt tgaaaccggt
     9301 cctaacggcc cattagttcc agaactgaaa gcacaattcc ccgatacacc ttacattgct
     9361 cgcccgggaa atattaacgc ctgggataac gaagattttg taaaagctgt caaagcgaca
     9421 ggtaaaaaac agttaattat tgccggtgtg gtaaccgaag tttgcgtggc attcccggcg
     9481 ctttcagcca ttgaagaagg gtttgacgtc tttgtcgtga ctgacgcgtc agggaccttt
     9541 aatgaaatta cccggcattc ggcatgggat cgcttgtcgc aagctggtgc gcaattgatg
     9601 acatggttcg gcgtggcgtg cgagctgcat cgcgactggc gtaatgatat tgaaggactg
     9661 gcgacgttgt tctctaacca cattcctgat tatcgtaatt tgatgaccag ttatgacacg
     9721 ttaacgaagc agaaataaaa gagaaccggg gccgaaaccc cggttgttat gattagcttg
     9781 cgacggccat ccccacggtc atatgcaaac catagaatac gccgcgaccg attaattccc
     9841 ctgccagaag cagaatgaat gatacagaaa gtagcggaac cgcaggctga taacctttta
     9901 gctgtggtgc aatccacaag cacagggcaa cggccaaaag cacgatccgc caggacatca
     9961 gcgcaccata gtccggcacc agcgctgcgg cctgctgcac agaactatga atagtcgcca
    10021 gctctgcgcc ctgcatcact gacaccacgc cactcactac cagtgccagt acagaaattg
    10081 ctggcagcag acgcatcgcc cagccatcga cgccagccag actcaacaac aggtaaccca
    10141 gtaacgggcc gcccataaac atcgtcagga agaagcccat cggcgtccag atgctgtacc
    10201 aggtcggaac ggtatcaatg ctgttataca cacgcaccat catccagaca aagatgacgc
    10261 caagaaccat cgtcactatc agccacagcg tacgcaatgc cggtgacagc tttttcagca
    10321 ttgccagcaa ccagccgatg ccgcctacgg caaaaaagat cgaaccgctg gcgatttcgt
    10381 tactgagtgc tgaagcccct acccggttga gcgagttaaa agcgcgcatt ggtgaaccaa
    10441 gatggagcat agaggcgata aagccaatgc ccatcagcac ccataaacca aacatgcagg
    10501 cgataacacg ctgctgggct tctgctcgca ggtcgccttt gagcagcgcc aaagccagaa
    10561 cgataaaacc acctgctaca cattgcccga agaccgtgaa gatcatcagc ggccattcat
    10621 gccatccact tcccatctca cacctccttc gggtttgcca gatagccagt ggtatccccg
    10681 gtcgggcggc tattggcatt gggtttgatc acaatattcg gtttggtaaa gtgagctcgc
    10741 ggcaacggcg caacggccgc cagatcgcca tgttttttac gcagctcgtc gatagggccg
    10801 aaatccagcg cccgcagcgg acaagattca acacagatcg gctttttacc ctccgcaaca
    10861 cggtcataac aaccatcgca tttggtcata tggcctttcg tttcgttata ttgcggtgcg
    10921 ccatacgggc aagccatatg gcagtagcgg cagccaatgc acacatcttc atcgaccaca
    10981 acaaaaccat cttcacgttt atgcatcgca ccgctcgggc agactttagt acaagccgga
    11041 tcttcgcagt ggttacatga aatcgacaga tagtaggcaa acacgttctg gtgccagaca
    11101 ccgttatctt cctgccagtc gccgccagca tattcataaa tgcggcggaa gctgacttct
    11161 ggcgtcaaat ctttgtagtc tttacaggcc agctcgcagg ttttgcaacc ggtgcaacgg
    11221 ctggaatcaa taaaaaatcc atactgggtt gtcatcggtt actccttaca ccttttcaac
    11281 ctgaacaagg tttgtatgtg acggattccc cttagcgaga ggagacggac gttgagtggt
    11341 cagtacgtta atacaaccac ccttatcgac acgttttgca tccgggtcat accaggcacc
    11401 ttcacccagt gcgaccacac ccggcatcat tcgtggcgtc actttcgcct caatatgaac
    11461 ctcaccacga tcgttaaaga tcctgacttt atcgccgttg tggataccgc gtttttgggc
    11521 atcaagcggg ttgatccaca tttcctgacg gcaagccgct ttcagcacat caacgttgcc
    11581 gtaagttgag tgaacgcgag atttatagtg gaaacctgta agctgcagcg gatactgttt
    11641 gttcagcgga tcctgataac tttcaaagcc cggcgtgtag atcggcagtg gatcgatcac
    11701 atcgccttca ggcaattccc aggtagcggc aatgtcagcc agcgcctgcg aataaatctc
    11761 aattttgccc gatggcgtag tcagtgggtt tgcctgcgga tcttcacgga aggctttata
    11821 agcaacgtga tgcccttgtg ggtcgcgctt tttaaagatc ccctgcttgc ggaactcttc
    11881 aaacgttggc agttcaggaa tcgcttcccg cgactgggca tacagatgcc gcatccactc
    11941 ttcctgggta cggccttcag taaactgttg ctcaacgcca agacgttttg ccagttcgct
    12001 ggtcatttca tagatggtct tacattcaaa gcgcggttta atcacctgat cgttgaaaat
    12061 cacgtaagac atattcccgc aggatgcatc cagcgcaaag tccatctgtt cggaagcggt
    12121 gcagtcaggc agcaggatgt cagcatattt cgccgatgag gtcatgtggc agtcgataac
    12181 cacaatcagc tcgcacttct tatcatcctg aaggatttca tgggtacggt tgatttcaga
    12241 atgctggtta atcaggcagt taccggcata gttccagatc attttgatcg gcacatccag
    12301 cttatctttc ccgcgtacac catcacgcag cgccgtcatt tccgggccac gttcaatggc
    12361 atcggtccac ataaacatcg aaatgctggt ctggatcggg ttttccaagg tcggcatacg
    12421 gacaaacggt aagctgtatg aaccttcacg cgcgccgctg ttgcctccgt taataccaac
    12481 gttaccggtc agaatcgcca gcatcgagat agcacgggtt gcgatttcac cgttagcgtg
    12541 acgctgcggg ccccatccct ggctgataaa cgccggtttg gtactaccga tttcacgagc
    12601 caatttgatg attttgtctg ccggaacacc agtgatttgc gaggcccatt ccggcgtttt
    12661 agccacgcca tctggccctt cacccagaat ataagcttta tagtggccat ttttcggcgc
    12721 actggctggc agggttttct catcgtagcc aacgcaatat ttatcgagga atgcctgatc
    12781 caccaggttt tcagtgatca tgacgtacgc cagaccgtta accagtgcgg catctgttcc
    12841 cggacgaata gggatccact catcttcgcg cccggcaccg gtgtcggtat agcgcggatc
    12901 gatgatgatc atgcgggcat tagatttctg gcgtgcctgt tcaagatagt aagtcacccc
    12961 accgccactc attcgcgttt cgccagggtt attaccaaac agcactacca gcttactgtt
    13021 ttcgatatcc gacgggctgt tgccatctgc ccagccgccg taggtatagt tcaaaccttc
    13081 cgcaatttgc gcggaggagt agtcgccgta atggttcaga tagccgccgc agcagttcat
    13141 cagccgcgcg accagggtat ttcccggcgg ccaggagcgg gtcatggtgc cgcccagcgt
    13201 accggtgcca tagttcagat agatagactc gttgccgtac tctttgatca ggcgctgcat
    13261 attggtcgcg atgatgtcgt aggcttcttc ccagctaatg cgctcgaatt tgccttcacc
    13321 gcgcgccccg actcgtttca tcggatattt caggcggtcc ggattgtaga cacggcgacg
    13381 catggaacgc ccacgcaggc aggcgcgaac ctggtgcagg ccgtcgtaat tgtcatcgcc
    13441 ggtattgtcc gtttcgacat atttgatttc accgtccacg acgtgcatac gtagcgggca
    13501 gcgactacca cagttaactg tacaggcgct ccagataacc ttttcgtctg attttgttgg
    13561 aatggcgcta tcgacagcgt gcgcaatccg actaaaaggt aatgttaatg cgctgctggc
    13621 cattgccagg ccgccgatcg ctgtcgtttt taccaaacca cggcgactca cctcagcagc
    13681 caataccgca tcagggattt tcgttttcat aatggctcac tcaagcttgc tcacgattga
    13741 ttccagtaac aaaccgtatt gtgtaattat ttctatagca gttcgctata taggcttgta
    13801 tacatcgaat aatttgaaaa tcaaagagat aggtagattt tcactcgaaa gtagtattac
    13861 gtgtaagtga ggagtaatta ttgttcggta tcaaagggag acaaaaaaag cgcccgcagg
    13921 agatttaacg gtacttactc ctcttgctga tttccttatt tttcaataag taaacaaaaa
    13981 caaggggagc aaattatcac aaataattac tggtcgatgg acagtttatg gacactttca
    14041 gccagcggat taaagctgat cgcatcctgt aaaaagtccg gtgaaaaatg cgcatacgtc
    14101 atcgtttgct gaatcgtggc gtgacccagg atcctctgca acgtgacgat gttcccgccg
    14161 ttcatcatga aatgcgtggc gaacgtgtgc cgcatgacat gcgtggcctg ccctttgggt
    14221 aagtcgggct tcacctcctg aaggatctcc ctgaaactga gataatcaac ctgatataac
    14281 aggccggtcc ggcgggtttt aattaacggc acaacggaat cagctacagg aaccgagcgc
    14341 gatttcccgt tctttgtgtt aaagaacgtc accctgttac ccacgatgtg ttccccgcga
    14401 agcgttgacg cctctttcca tctggccccg gtcgaaaggc acagaatggc aatgcgtcgt
    14461 gcatcaccct caagccgttt cagaaggcga tcaatctcgt catctgaaag aaaggccatt
    14521 tctgtattct gaaccttgag tttacgtatt ccgcgcacag ggttttcgtt atgataaacc
    14581 tccgcgtcaa ttaacaacga aaacatcgca gataaggcgc acaggtcgcg attgatactg
    14641 gacggcatca gtcccgcctg caacttatcc gagcgatact caatcatgaa ttttcgtgtc
    14701 atctggctgg cgcgcgggtc ccccatttca cgtatcacct tctccagtct ggttctgtag
    14761 gtatcaccat atgcctgatt acgaccatca agcatccacc acacctcaat cagttctgat
    14821 aaccgccgtt gatcggcggg ccttgcctgc cacgggttat tgtggaaatt ctgaagaacg
    14881 tatctttcga attcctgcgc ctttgatttc agggtgaaaa tcttacgaat ccgccttcct
    14941 cccgccccct gaggccttac atcaacctga taacgtccgt ctttgagttg cttaatcgac
    15001 atgatcagca cctccgacgg ataaacctgt taacccctgc ttactgaacg ccgcccgttc
    15061 aatatacagc gtcagccagc attgcggcct gattggggtg atttttgggt attgcgggtt
    15121 gaaacctgat cgtcctccga ctgcgtggag ccatcaagag agagagccgg ggcgatctga
    15181 ccggcaacag gtagcgtctt atctttgatt aaccagaaca tatatttttc aaaccgatcc
    15241 acttgaagga cggcctcaac aatctttgct ctggcgatct tttgccctga ctcataattc
    15301 ctgacagttg ccagagataa ccctgtaata tcagcaaatt gcttttgagt tagcccttcc
    15361 gtcttgcgta tcaaacgcaa ctttttcgca tagtctattg acaagatact catttgggga
    15421 ttaatctcct acaaaagata accgtacggt tatctttaca agtcatcaaa aatcatcaaa
    15481 aggcgcaaca agcgcaccaa tgcaagagga taacaaatgc gggagattat tgccgacaaa
    15541 gagttatctg gcgctcaacc ggttcaggat gatggcgcgg acaatgaatt cattgaagct
    15601 gccaacgaca acgaacacct ggaaaccaaa accagaccgt cctacaaaaa gggcgcaacc
    15661 ctacgactgg acggcccagt cgcaggactt gtgtcactgg aaaaaggggc tgcctatctg
    15721 ggggtaacaa aatctgcgct acgcgccgcc attcagcgcg ggcagatgcc gggtcacaaa
    15781 acgcgctcaa atcccgaaga tgaaaagtca gacggtacat ggtggtttaa cgccaaagcc
    15841 tgggacgaac tagccgacga actactggag tgcgaaccgc cagagtggca caactggaaa
    15901 agctactgga cgtacgaccg caagaaaaga aagttttcac cggcaaacaa ggaggactgc
    15961 cagaccgtta aaggtaagca ggtttactcc cccagagttt caaaactgga gcaactcaaa
    16021 aacaaccagc ccggctaaac acgaaaatga ccagcaatat catcagcgcc attctgacac
    16081 ttgtttttat tgagataggc gtaatagcga tctatttgtt tcgtaagtta acgggacatg
    16141 aagaacgctt tattgaactc agcattgaat atatcgccgc ttataccaaa ggacttttcc
    16201 cggcagctat tggggcgatg tttatagcgt ttgttatctg gtttatcggg tgagaaacct
    16261 gataaaccac aactactgca atgagggtaa ttatgtgtgg catggacaaa ttggacctta
    16321 tgttaattgt aatactatca attaacttcg gttacctctt aagcggcgta attctcatgt
    16381 gcggaggcaa acgaaaatga accagcagtc cgcaaaacgt gaacacaatg cgatgcgatt
    16441 taatcgcaaa tattttgagt tcgccgctat gccggaataa ttaaatctgt acagagtctt
    16501 taacatgaaa cagcaacgta attcacgctt tcgtaatggt gctgaacgtc acgctaaccg
    16561 tttcgctacc agtgcatcac gtagtaactc ccgctacagc ctgagcgaga cacacgcaac
    16621 accgggcggt catgctgtaa aacagactgg tcaacacacc tggctgattg agcaggccgg
    16681 actcgtgatc cacagatgcc accgcaatcc gtttaccgga aaccgcattt ttgcactgag
    16741 cagcggcgac aatcagttcg gacaggattt cacactgcac gaagccttgc gaactgccga
    16801 ccgcttaatc gaggggcgca gctttcagca taccgactac taaggcaaaa ggagtcttaa
    16861 taagacagaa gaggcagcaa gatgaaaaga gcctataaag aactcattaa ccacgcgctt
    16921 cagaattacc attacggact tacaagccgt ggcactaacg gtgacgctgt ttcacttgca
    16981 gcactgacta cagcaatcaa gggcctgacc tgttgcgcct ttggttccgg agataatgaa
    17041 gctctgacgg aattacaggc cattaataaa gacctgtctg aaggaaacgt aataaccccc
    17101 tttgtaattc aggaggaagc atgagcgtat ttatgtcatg gttaattctt atccttgcca
    17161 ttatttgcgc tgtggggatt atgcgggtaa ttaacagcat taacaccatt gaatcgttat
    17221 ttactggcga taagcgccgt aaataaacag gcgtttaaca ccagaaacaa ccagaaacat
    17281 ccgctcacgc ggaggggttg acccgctcaa aaaggagaaa tcaggaatgc ttaataataa
    17341 aaaaaaccga atcattgata acagtgaaca ggtcaacaaa ataatcgaag gcacaaacaa
    17401 attaattaag aaaaaaaaca aactaagccg tgaattaatg gaacatccgg aagaaatatt
    17461 attagccatg atgcaccaga cagaagcgat agccatcgcc cttgccgtgc aggcgctttc
    17521 tgagttccgg gaagaaaaca tcaggctggc tcaggagctg aaaaacctca gacgtcagct
    17581 tcagagcact gaggctggca cgtcatcacg aatgcactga gggccacacc atgactgcaa
    17641 ttctgagaag acgcaataaa acgcttttta ccgatacatc cgggatggaa tacgaagtgg
    17701 aaagcagtgt catagccacc accacccgat gcccggcagg tgacgaactg atttacgtcc
    17761 acctgacgga cgggtcacag ataaccgtcc tgacagagtc atggcgcgaa ctcgagataa
    17821 taagcgaggt aagaacatga gcagcaaaca cgataccaac gcacgcagac caggcaaaga
    17881 ggcgcgtcac catatcgccg taatgcgcaa cctgcttatg agtgaaatcg acgattttgt
    17941 ggcaggcctg gggcaaccgg gcgaaccagc aacaccaggc cagatacaca gggaattacg
    18001 ccgccgcgtg gaaaacgtcg tggattacgt cataaacccg aacgactgac gggaacatgg
    18061 cgagcgtcag atgacacacg cagggcactt atcgggacaa accgatcgta atcgcagcca
    18121 tcatcttttc agcgaaagag agagattcac gatgactgat attcagagac atacaggctt
    18181 tgttgtacgc cacattttcc atgcattcca ggacaggaga gtcatgcatc tcaagctctg
    18241 gaaggcgtgc cagcagagct tccgtactga ggagggcgct tttggtaatc aactcattgt
    18301 agcgatgggc atgctcactc gccgcctgcg cacgttgccc gaaacgacat atgaaattac
    18361 aggctccgag aaccaccacc actgccccca gcacccagcc gaaatgccag ccggctacga
    18421 tgaacgcacc cgccgcaagg atcaaaatgt tgatgatcgc atcaaggcga cgattgaaca
    18481 ccgccgcgat gcgctccagc cagtagctgt accagagacg gaacatgacc tcatcccgtt
    18541 ctctcgattt tttacgttgg ttgttttgca tggtgtttta tccctcatcg tcatcgctct
    18601 gcccctaatc agcgcagagt gcagggtaac ctggctcgtg gaatttttgc agccagacat
    18661 ctttaccccc gggcgtgttg aacaaagtgt ccgccagatc cactgaacgg cggattcatc
    18721 aatgctaaca cgcccttctt ttccggttga aggcgaaaaa accggaccat taccccgaca
    18781 caaaacagaa caacggagaa caactatgcc cgcacttaaa cctatgggtg tatcaggtgt
    18841 accaccgaag catgtcagag cctggacaca acaggaagat gctctactgg tcggtttata
    18901 cccctcccac accgtaccag aactgacaaa acactttcag agaaccgggc cagcaatacg
    18961 aagccgcatc cgtcttttgc tctctcaggg ggttatccac accaa
//
LOCUS       DBBGLQ010000084        18587 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.084, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000084 DBBGLQ010000000
VERSION     DBBGLQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.084"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            241..1125
                     /locus_tag="ACIYSB_003870"
     CDS             241..1125
                     /locus_tag="ACIYSB_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGC5430817.1"
                     /translation="MSFSHSSLSAHVKSHLTFLPEEIRQKILEYLHSVIQYEPVIGIM
                     GKSGAGKSSLCNAIFQSRVCATHPLNGCTRQAHRLTFQPGERRMTLVDLPGTGETPQH
                     DQEYRALYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     IHPAEEWNSQSNTPSRQQELSLATVTARVATLFPSSFPVLPIAAPAGWNLPAFVSLMI
                     HALPPQATSAVYSHIRNEKRTAQDQQHAQQAFGDAIGQSFDAAIARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDRLF"
     gene            1311..2129
                     /locus_tag="ACIYSB_003871"
     CDS             1311..2129
                     /locus_tag="ACIYSB_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGC5430818.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSRNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGQDLWS
                     AYQTIQENMLKGGLQGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            2226..2699
                     /locus_tag="ACIYSB_003872"
     CDS             2226..2699
                     /locus_tag="ACIYSB_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGC5430819.1"
                     /translation="MKTVSHDSVSATTASGNNPPQLVATTVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYSGGIWNMYTLNNGGAFMAPEPGNDDDETWVMFNAMNGN
                     RAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2715..3191
                     /gene="radC"
                     /locus_tag="ACIYSB_003873"
     CDS             2715..3191
                     /gene="radC"
                     /locus_tag="ACIYSB_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGC5430820.1"
                     /translation="MQQISFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILHMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVVKRALYHNAAA
                     VVLAHNHPSGEVTPGKADRLITERLVQALALVDIRVPDHLIIGGSRVFSFAEHGLL"
     gene            3281..3502
                     /locus_tag="ACIYSB_003874"
     CDS             3281..3502
                     /locus_tag="ACIYSB_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGC5430821.1"
                     /translation="MKIVTRGEAMRVHRQYPSSRLFPFCTGKYRWHGGTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3519..4163
                     /locus_tag="ACIYSB_003875"
     CDS             3519..4163
                     /locus_tag="ACIYSB_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HGC5430822.1"
                     /translation="MSSIITTTVYTLNELSATAREKARGWYRQHHADSRWYENVYEDF
                     RAVCEIVGIVLRQRVIRLSSGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATARRVR
                     EYAPQDRELHRITDALQAVQKHNFRQLQAEIRHRGRYCHPYSMDITVTRDSPTGQAMT
                     AGAETVVCDALRDLAFWLYSQLENEYDWLTSDDAVDEALLINGYTFTEAGLRAG"
     gene            4214..4591
                     /locus_tag="ACIYSB_003876"
     CDS             4214..4591
                     /locus_tag="ACIYSB_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGC5430823.1"
                     /translation="MSDTLTGTTHPDDNSDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSGELSPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYIAIYPTPATPGTTA"
     gene            4638..5015
                     /locus_tag="ACIYSB_003877"
     CDS             4638..5015
                     /locus_tag="ACIYSB_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGC5430824.1"
                     /translation="MKTFPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSASTRSQLISSIDILRARRATG
                     LMTRSHYRMVNNITLGKHCEVKP"
     gene            5012..5500
                     /locus_tag="ACIYSB_003878"
     CDS             5012..5500
                     /locus_tag="ACIYSB_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGC5430825.1"
                     /translation="MKLSLTLEADSVNVQALNMGRIVADVDGVNLAELINVVCDNGCS
                     LRVVDESDRTSADCMPPFAALTGIRCSTAHITEQDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPALRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDAGAECLPGLPTF
                     DW"
     gene            5509..5706
                     /locus_tag="ACIYSB_003879"
     CDS             5509..5706
                     /locus_tag="ACIYSB_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGC5430826.1"
                     /translation="MKSLTTETAPDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQARADIRTLRHLQLQHQNR"
     gene            5802..6650
                     /locus_tag="ACIYSB_003880"
     CDS             5802..6650
                     /locus_tag="ACIYSB_003880"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGC5430827.1"
                     /translation="MSDITIPYPEAVNGHTDVICSTDIRHVVTVRNEALQQTERVIRQ
                     LAEISAMTESIGGKTARDWAVKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDEWYRSQEDDNFPEISEANILSTSEQLHRNRDEMAERGVINLFRGLS
                     RTQNSGSPCKFGSKIILNNLVKWDWWGFGLNHGPQAGRLADLERMLYLFSGKPIPDYR
                     DNILIRLDEHIRSVQGEACYEDEMFSIRYFRKGSAHITFKHPELVEKLNAVLAKHYSV
                     KFHSSV"
     gene            complement(6911..7669)
                     /gene="phnP"
                     /locus_tag="ACIYSB_003881"
     CDS             complement(6911..7669)
                     /gene="phnP"
                     /locus_tag="ACIYSB_003881"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGC5430828.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(7671..8105)
                     /gene="phnO"
                     /locus_tag="ACIYSB_003882"
     CDS             complement(7671..8105)
                     /gene="phnO"
                     /locus_tag="ACIYSB_003882"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HGC5430829.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(8092..8649)
                     /gene="phnN"
                     /locus_tag="ACIYSB_003883"
     CDS             complement(8092..8649)
                     /gene="phnN"
                     /locus_tag="ACIYSB_003883"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGC5430830.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(8649..9785)
                     /gene="phnM"
                     /locus_tag="ACIYSB_003884"
     CDS             complement(8649..9785)
                     /gene="phnM"
                     /locus_tag="ACIYSB_003884"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGC5430831.1"
                     /translation="MIINNVKLVLENEVVSGSLEVQNGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            complement(9782..10462)
                     /gene="phnL"
                     /locus_tag="ACIYSB_003885"
     CDS             complement(9782..10462)
                     /gene="phnL"
                     /locus_tag="ACIYSB_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGC5430832.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWMSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVGLIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(10573..11331)
                     /gene="phnK"
                     /locus_tag="ACIYSB_003886"
     CDS             complement(10573..11331)
                     /gene="phnK"
                     /locus_tag="ACIYSB_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGC5430833.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWQEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(11328..12173)
                     /gene="phnJ"
                     /locus_tag="ACIYSB_003887"
     CDS             complement(11328..12173)
                     /gene="phnJ"
                     /locus_tag="ACIYSB_003887"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGC5430834.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(12166..13226)
                     /gene="phnI"
                     /locus_tag="ACIYSB_003888"
                     /pseudo
     CDS             complement(12166..13226)
                     /gene="phnI"
                     /locus_tag="ACIYSB_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
     gene            complement(13226..13810)
                     /gene="phnH"
                     /locus_tag="ACIYSB_003889"
     CDS             complement(13226..13810)
                     /gene="phnH"
                     /locus_tag="ACIYSB_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGC5430835.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(13807..14259)
                     /gene="phnG"
                     /locus_tag="ACIYSB_003890"
     CDS             complement(13807..14259)
                     /gene="phnG"
                     /locus_tag="ACIYSB_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGC5430836.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVQGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(14260..14985)
                     /gene="phnF"
                     /locus_tag="ACIYSB_003891"
     CDS             complement(14260..14985)
                     /gene="phnF"
                     /locus_tag="ACIYSB_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGC5430837.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(15006..15793)
                     /gene="phnE"
                     /locus_tag="ACIYSB_003892"
                     /pseudo
     CDS             complement(15006..15793)
                     /gene="phnE"
                     /locus_tag="ACIYSB_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
     gene            complement(15899..16915)
                     /gene="phnD"
                     /locus_tag="ACIYSB_003893"
     CDS             complement(15899..16915)
                     /gene="phnD"
                     /locus_tag="ACIYSB_003893"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGC5430838.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(16940..17728)
                     /gene="phnC"
                     /locus_tag="ACIYSB_003894"
     CDS             complement(16940..17728)
                     /gene="phnC"
                     /locus_tag="ACIYSB_003894"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGC5430839.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            complement(17861..18303)
                     /gene="yjdN"
                     /locus_tag="ACIYSB_003895"
                     /pseudo
     CDS             complement(17861..18303)
                     /gene="yjdN"
                     /locus_tag="ACIYSB_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
BASE COUNT         3838 a         5335 c         5398 g         4016 t
ORIGIN      
        1 ttcctgaccc gacggttctc tttatttcgt ttccataact gataatgatc atcacataac
       61 ctcagggcat ttgcctcatc aaattctgaa acaggattgc tgcatttttt attccgacag
      121 atacctttgt ttttagccat actcttcttc ccgtcaatgg aaaatttttc acgtccatat
      181 tacctgactg agagaccgga ttaatgtgat ccggttcagt gaaatcaaca ggataccggt
      241 atgtcattca gccattcttc cctttccgca cacgtaaaat cacatctgac ctttcttcct
      301 gaagaaatac gccagaaaat ccttgaatat cttcacagcg tcattcagta cgaacccgtg
      361 attggcatta tgggtaaatc cggtgccgga aaaagcagcc tgtgcaatgc catttttcag
      421 tcccgtgtct gcgccacgca tccactgaac ggctgcaccc gtcaggctca tcgtcttacc
      481 tttcagcctg gtgaacgcag aatgacgctc gtcgatctgc ccggcactgg cgaaacacca
      541 cagcatgatc aggaataccg ggcactttac cgtcagttac tgcccgaact ggatctgatt
      601 atctggatcc tgcgtgctga tgaacgtgcg tatgctgccg acatcgccat gcatcagttt
      661 ttactgaatg aaggtgccga tccctcgcgt tttctgtttg ttctcagcca tgctgatcgc
      721 atccacccgg ctgaagaatg gaacagccag agcaacacgc catcccgtca gcaggaactc
      781 tcactggcaa cagtaacagc ccgggtggca accctgttcc cttcatcatt tccggtactc
      841 cccatagctg cccctgcagg ctggaatctt ccggcatttg tgtcactgat gatccacgcc
      901 ctgccaccac aggcaaccag cgcggtttat tcgcatatcc ggaatgaaaa gcgcactgcg
      961 caggatcagc aacacgcaca gcaggctttt ggtgacgcca tcggccagag ctttgacgcg
     1021 gccattgccc ggttcagctt tccggcctgg atgctgcagc ttctgcgtaa agcccgtgat
     1081 cgcattatcc gcctgctggt cacactgtgg gatcgcctgt tctgaatttc cccgcttttt
     1141 gaccggcaca gacgatgacc agggaacgca tttccggcgg ctttaccaca atgctgaagg
     1201 gcttttggta aacctcattc atttcatcca tcaggcagcg tcttctccgg gagatgcggc
     1261 tttttcattg attacgtcag tcagtcttaa ccactgataa ggagcaaagt atgcgattag
     1321 ccagtcgttt tggttatgcg aaccagatac gccgtgaccg tccgctgaca catgaagagc
     1381 tgatgcacca tgtgcccggg attttcgggg aggataagca cacctcccgg agccggaact
     1441 atacatacat ccccaccatc accgtgctgg aaagcctgca acgggaaggc tttcagccgt
     1501 tctttgcctg ccagacccgc gtgcgtgacc cgggacgccg gggatacaca aagcacatgc
     1561 tgcgtctgcg ccgtgacggg gagataaacg gacagcatgt ccctgaaatc atcctgctca
     1621 actcccatga cggcacctcc agctaccaga tgctgccggg ttacttccgg ttcgtgtgtc
     1681 agaacggatg tgtctgtggt cagtctctcg gagaggtgcg tgtcccgcac cggggagacg
     1741 tggtggacag ggtcattgaa ggggcttacg aggtggtggg cgtgtttgac cggatagagg
     1801 aaaagcgtga tgccatgcag tcactgattt taccaccacc ggctcgtcag gcgctggcac
     1861 aggccgccct gacttaccgt tatggtgacg aacatcagcc cgtcaccacc gccgacattc
     1921 tgacaccacg acgccgggag gattacggtc aggacctgtg gagtgcttat cagaccatcc
     1981 aggagaatat gctcaagggc ggtcttcagg gacgcagtgc aaaaggaaaa cgtatccaca
     2041 cccgggccat tcacagcatc gacaccgaca ttaagctcaa tcgtgcattg tgggtgatgg
     2101 cggaaacgct gctggagagc ctgcgctgat taacgcgacg ctgtcagtgc cctgtctttt
     2161 ctctcttatt cattcactta ttttcttaat tcacttgttc acttattcac ctgaggattt
     2221 cacttatgaa aacggtttcg catgactctg tttccgcaac cactgcatcc gggaataacc
     2281 cgcctcagct ggttgccacc acggtgcctg acgaacaacg catcagcttc tggccgcagc
     2341 actttggcct cattccgcag tgggtgaccc tggagccccg tatcttcggc tggatggacc
     2401 ggctgtgtga agactacagt gggggtatct ggaatatgta caccctgaac aacggcggcg
     2461 catttatggc cccggaaccg ggtaacgatg atgacgaaac atgggtgatg tttaacgcca
     2521 tgaacggcaa ccgcgcggaa atgagcccgg aagccgccgg cattgccgcc tgtctgatgg
     2581 cctacagtca tcacgcctgc cgtacggagt gttatgccat gacggtccat tattaccggc
     2641 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatt atcgactgaa
     2701 tggaggcagg ggcaatgcaa cagatttcct ttctgcccgg cgcgatgacg cccggcgagc
     2761 gcagcctcat tcagcgggcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     2821 ccttcacctc cacgcatgcg gcacgggaat ggctgattct gcacatggca ggactggagc
     2881 gggaagagtt ccgggtgctg tacctgaaca accagaatca gctgattgcc ggtgaaaccc
     2941 tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaggtggtt aaacgcgccc
     3001 tgtaccacaa tgccgctgcc gtggtgctgg cgcacaatca cccgtccggt gaagtcacac
     3061 ccggtaaggc agaccggctt atcactgaac gtctggtaca ggcactggcc ctggtggata
     3121 tccgggtgcc ggaccatctg ataatcggtg gcagccgggt gttctccttt gccgaacacg
     3181 gtctgcttta atccgtcacc gtcacaacca ccttcatatt gtcacttcag tttctctttc
     3241 tcagctgttt ctcactttca cattcaggag gactgttctc atgaaaatcg tcacccgtgg
     3301 tgaagccatg cgcgttcacc gtcagtatcc gtcatcccgt ctttttccgt tctgcaccgg
     3361 taagtaccgc tggcacggcg gcactgaggc gtataccggt cgtgaagtgc aggatatccc
     3421 cggcgtactg gccgtgtttg ccgaacgccg caaggacagt tttggcccgt atgtccggct
     3481 gatgagcgtc accctgaact gagtcagtgg agatgctgat gagcagcatt atcaccacca
     3541 ccgtgtatac gctgaatgaa ctttccgcca cagcacggga aaaagcccgc ggctggtacc
     3601 gtcagcatca tgctgacagc cgctggtatg aaaacgtgta tgaggacttc agggcggtct
     3661 gtgagattgt cgggattgtc ctccgccagc gtgttatccg gctcagcagc ggcaggttta
     3721 tggaagagcc gtgcatctgg ttcagcggat tctgcagcca gggggacgga gcctgttttg
     3781 aagggcgctg gcactggcag ccggcaacag ccaggcgagt ccgggaatat gccccgcagg
     3841 accgtgagct tcatcgtatc acggatgccc tgcaggcggt gcagaagcat aacttccggc
     3901 agttacaggc tgaaatccgt caccggggac gctactgcca tccgtacagc atggatatca
     3961 ccgtcacccg ggacagcccg accggacagg caatgacagc cggcgcggaa accgttgtct
     4021 gtgacgccct gcgggacctg gccttctggt tatacagtca actggaaaac gagtacgact
     4081 ggctgacctc agatgacgcc gtggatgagg cgcttctcat caacggatac accttcaccg
     4141 aagccggtct gcgcgccggc tgacccgtca ttgttaaaac cattcttaac cgattatcag
     4201 tgaggacttt atcatgtcag acacactcac cgggacaacg catcccgacg acaacagcga
     4261 ccgcccctgg tgggggctgc cctgcaccgt gacgccctgt ttcggtgccc gtctggtgca
     4321 ggagggtaac cgcctgcatt accttgcaga ccgcgccggc atcagaggcc ggttcagcga
     4381 cgcagatgcg taccacccgg accaggcctt tccgctgctg atgaaacagc tggaactcat
     4441 gctcaccagt ggtgaactca gtccccgcca tcagcatacc gtcacactgt atgcgaaagg
     4501 gctgacctgc gaagccgaca ccctcggcag ttgtggttac gtgtatatcg ccatttatcc
     4561 gacaccggca acacccggaa ccaccgcata accccggaca gcacttcttt attatcacct
     4621 gttacagagt acttaagatg aaaacattcc ccgacacaca cgtacgggcg gcatcgcgct
     4681 gcccgtctcc ggttgaaatc tggcagacac tgctcacccg gttgctggac cagcactatg
     4741 gcctcacgct gaatgacaca ccgttcgccg atgaacgtgt gattgaacag catattgagg
     4801 caggtatttc actgtgtgat gcggtgaact ttcttgtgga aaaatacgcg ctggtgcgta
     4861 ccgaccagcc gggattcagc gccagtaccc gttctcagtt aatcagcagc attgatattc
     4921 tccgggcccg ccgggcaacc gggctgatga cccgcagcca ctaccgaatg gtgaacaaca
     4981 tcaccctggg taaacactgt gaggtgaaac catgaaactg tccctgacac tggaagccga
     5041 cagcgttaac gtgcaggcac tgaacatggg gcgcattgtc gctgacgtcg atggtgttaa
     5101 tctcgctgaa ctgattaacg tggtctgcga taacggctgt tcccttcgtg ttgttgatga
     5161 atctgaccgg acctcagcag actgcatgcc accatttgcg gcccttaccg gcatacgctg
     5221 cagtaccgca catatcacgg aacaggacaa tgcctggctg tactcgctgt cgcatcagac
     5281 gaatgacacc ggcgaatcag aatggattca ttttacaggc agcggatatc tgttacgtac
     5341 cgatgcgtgg tcgtacccgg ctctgcggct taaacgcctg ggtctgtcaa aaacgtttcg
     5401 tcgtctggtt gtcacactca tccggcgtta tggcgtcagt ctcatccatc tggatgccgg
     5461 cgctgaatgt ctgccgggtt tacccacttt cgactggtaa ccgacagtat gaaatcatta
     5521 accacagaaa cggcaccgga tattctgatt gcgtggctgc aggacaatat cgactgcggc
     5581 tccggcatta tcttcgacaa cgatgaggat aaaacggatt cggcagcact gctgccctgt
     5641 atcgaacagg ccagagcgga tatccgcacc ctgcgccacc tgcagcttca gcaccagaac
     5701 cggtgagtct cactcatcat ctcactcacc acgattcatt cccctgacgc cagtccgaat
     5761 acggctggcg ttttcattta taaaccacaa ggaaaaatgt aatgtctgat atcacaatcc
     5821 cttatccgga agcggtcaac ggacatacgg acgttatctg ctccacagat atccggcacg
     5881 ttgtgacggt acggaatgaa gcactgcaac aaaccgaaag ggttatccgg caactggcag
     5941 aaatctcagc aatgacagaa agcataggcg gtaaaacggc gcgggactgg gccgtgaaac
     6001 aggattttcg ctgtggctgc tggctgatgg aaaaaccgga aaccgcaatg aaagccatca
     6061 cccggaacct tgaccgtgaa atctggcgtg acctgatgca acgctccggg atgctgagtc
     6121 tgatggatgc acaggcgcgt gatgaatggt accgttcaca ggaggacgat aatttcccgg
     6181 aaatcagcga agcgaatatc ctgagcacgt ctgaacaact gcaccgtaac agggatgaaa
     6241 tggctgaacg gggcgtcatc aacctcttca ggggactgag ccggacgcaa aacagcgggt
     6301 caccctgtaa attcggcagc aagattatcc tcaacaacct ggtgaaatgg gactggtggg
     6361 gatttggtct gaaccatggc ccgcaggccg ggcggcttgc cgacctggaa agaatgttgt
     6421 atctgttcag tggtaaaccc atacccgatt acagggacaa tatcctcatt cgtctggatg
     6481 agcacatccg gtccgttcag ggtgaagcgt gttatgaaga tgagatgttc agcatcagat
     6541 acttcaggaa aggctctgcg catatcacgt ttaaacaccc ggaactggtg gagaagctga
     6601 atgctgttct ggcgaaacat tattcggtaa agttccattc ttctgtataa acttacagat
     6661 cacgggtatt gtgtctcgtc ctgaacaggg ccgacaggta tactgcattc gctgagccat
     6721 tacagataat gcctcaactg agttcaatct acctccatgt acaacaacaa tacgatgggg
     6781 gctacattgg gggctatact ttcaagtaaa actgatataa ccaagattaa acaataagat
     6841 agagaatttt caccattccc ctctggggag agggttaggg tgagggggcc agcgcgcaca
     6901 ttccctctca tcacgccacc ccaatctcca tcccatcaaa ccccacctca aaccctgacg
     6961 gtagtgcgtt ttccatcagc cacgcatcaa actggtggct gatatgggtc agaatcaccc
     7021 gtggcgagcg gataacctga ttcagcgcaa gcacggtatt taaatcacag tgattacgcg
     7081 gtgcatccgc gcgcggcggg tgactgcaat ccatcaccat tacctgcggc tgattattgc
     7141 gtaaaaattt cagcgttttt tccggcaagc ctgcggtgtc agacagccac gccacccggc
     7201 tgtgtgccgt ttccagcaga taaccgaagg tcagttttga gtggttgagc ggcaggggcg
     7261 tgacctgtaa cccctgcaaa tcaaacacca caaacggttc caccgtgtgg ctgaaatcaa
     7321 gcaggcccgg atgtttaaac agatcgtcgc agccctgttc atccggcggg ccgtaaaccg
     7381 ggatcggatc gccaacgccc cagcgcagcg gaaacagccc ctggacgtga tccatatgat
     7441 aatgcgtcag caaaaactgc tggaacgatc cgggcgacca gcgatcggcg agatcgtgca
     7501 gcccggcgtc gatcagggtg attgcgtcgt taaacttcac tacgccgctg cacggttggc
     7561 ggcgatactg cggcgagcgc cgcgctctgg cgcaggccgc acactcgcag ccccatgccg
     7621 gaacgccctg tgcgccgccg gtgccggtga gcgtgagggt caggctcatg ttacagcgcc
     7681 ttggtgaagc ggaagtggct ctgctcgtag ccttcgcgca gatagaaacg gtgcgcgtcg
     7741 tggcgcttca cgttggtcga aagttcggtc atttcggccc cggcctggcg ggcttcttct
     7801 tctgcccacg ccagtaactt actgccgacg ttcagaccgc gcgcctgcgg cattaccacc
     7861 aactcctgaa tttcgccgat ccagttgaca tgatgcagat gaaactgcaa atgcaggccg
     7921 atcatgccga caacttcgcc atcaagcagc gccagatggt agcgcatgtt tgggtcgcgc
     7981 agattggcgt taaaacccac gcgaaacgcg tggtggtcaa actccgcctg ttttagctca
     8041 caaatcagcg cgtaaaccgc gtcggtgtcg tactgcgtgg ccgggcgaag ctcacaagca
     8101 ggcatggtgt ttctccttct gatggatcag cgtcagcagc gtgtcgaccg actggcgcag
     8161 gctgccgtca ttgttgagcg tatggcaatc ctgtggagta tagcgggcgg cgcgcgccag
     8221 gcgggcgtta atttcactgg cgttttcacg gccacggttt tccaggcgct ggcggaggat
     8281 ctccggcgaa acctgtaaac agacgggcag cagcgccgat tgatagcgcg cccgcgcctg
     8341 cggcagatgg gcgcgtgagc cgttgaccag cacgtcgaat ccggcgtgca gccagagatc
     8401 aatctcgacg ccgacgccat aatacagacc gttagcgtgc cagcttaagg ccaacagatt
     8461 ttgccccgcg cgggtaaaaa actcctgctc gctcagggcg atatggtttt cacttccggc
     8521 gctggcatcg cgcgtgatgt agcgatgcgc caccagtaac tgagtttgtt cccgcaggcg
     8581 gagttccgcc agcaggctgt ctttcccgga gccggacggc cccattaacc aaatcagttt
     8641 tcccatcatc agaacaccct tttaccctga cgccagacgt ggtcgatatg aatatgattg
     8701 tccttgcgat gcgccagcac caggtcggcg cgtttgccct cgccaatcac cccgcgatcc
     8761 tggagattaa gcgcctgcgc tggattttta gtcaccagct tcaccgcctg cggcagcgta
     8821 aagcggttgc tctggtcatc ggcgacgcga aatgccgcat cgagcaggct ggcggggtag
     8881 tagtcggaag agaggatatc cagcaggcca agctgcgcca gttcactggc cgccacgttg
     8941 ccggagtgcg agccgccgcg cacaatattc ggcgcgccca tcagcacgtt catgccatgc
     9001 ttgcgcgagg cttccgccgc ttcgaacgtg gtgggaaatt cggcgatcac gctgccaagc
     9061 tggtgagatt cagcaacgtg ggcgtgggtg gcgtcatcgt ggctggcaag cgcaattttt
     9121 cgcgcgcggc acagggcggc gatggattcg cgattcggct gcgaccagcg tgcggcgagc
     9181 gccagttgct cttcttcgta ctgctgcatc tgcgcatcag tgagggagta tttgccctga
     9241 taatattcgc gatacttctc gcggttggcg aactggcgct ggcccggcga gtggtccatc
     9301 agcgacacca gcgtcaccgg ctcgcgctgc accagttttt caaacagcgg cagcgtggtg
     9361 tgatgcggca gttcgcagcg cagatgcaga cggtgctcgg cgcggttgac gccgcgtttc
     9421 tgcgtctctt cgatggcgtt gatcatcttc tccagattct ccagccgatc gccgccgtcg
     9481 cgcacgtcgc caattgccac ggcatccagt acggtggtga tgccgctcgc caccatcagc
     9541 gcgtcgtggc tgctcatcgc cgagtgggca ggccagtcaa ctttcgggcg cggggtgaag
     9601 aatttatcca gattatcggt atgcagctca atcagccccg gcagcagcca gccgccttcg
     9661 ccgtccatcg cctccggcag gcggctctgg ctttcggcaa aggcgcggat ttcgccgttc
     9721 tgcacctcca gcgaaccgct taccacctcg ttttccagca ccagcttaac gttattgata
     9781 atcatgaaga ggctcccatt gggtgcaggc ggtcggcgac gtcattacgt acagcttcgt
     9841 catggaagat gcctacgatg gctgcgccac gggttttggc ttcgcgaatc agtcccacca
     9901 ccgcggcgct gtttttggcg tccagcgagg cggtaggttc gtcaagcagc agaatggggt
     9961 agtcgacgat aaagccgcgg gcgatgttga cgcgctgctg ttcgccaccg gaaaatgtcg
    10021 atggtgccag gtgccacagg cgttccggca cgttcaggcg ggtgagaaga cgcgcggctt
    10081 tagcggcgca ggcttcacgc ggaacgccgg tatcgagcag cggctgcatc accacttcca
    10141 gtgctgagat acgcgggatg acgcgcagaa actggctcat ccagccgacg gtggttttgc
    10201 ggatttccac cactttgcgc gctggcgcgg tgaccaggtc tacccactcg tcaccgtgtt
    10261 tgatctggat ttgaccttcg tcgggcagat agttggcgta cagcgagcgt agcagagttg
    10321 atttgccgct gccggaatgg ccgtggagca ccacgcattc gcccgcgttg acggtgagcg
    10381 aggcgcgatt gaggacgggc aggcgcacgc cgttttgctg gtgcaggatg aaggttttac
    10441 tgacgttttg tacgttaatc atttttcgcc tcgtggttcg ggtgtcggat gcgacgctga
    10501 cgcgtcttat ccgacctacg gggagcgcat ttgtaggccg gataaggcgt ttacgccgca
    10561 tccggcaccg gctcaattct gcaaaaccga tgacaccagc agctgtgtat acggatgatg
    10621 cgggtcgtcg agcacgcggt cggttaaccc actctccacc acttgcccct gcttcatcac
    10681 cagcaaacgg tccgccagca ggcgggcgac gcctaaatca tgggtgacaa tcaccaccgc
    10741 gaggttcagc tccaccacca ggccgcgcag caggtcgagc aggcgggcct gcaccgacac
    10801 atccagcccg ccggtcggtt catccataaa caccagcttc ggatgcgtca ccaggttgcg
    10861 ggcaatctgc aaacgctgct gcataccgcc ggaaaaggtg gtcggcaggt cgtcgatccg
    10921 gttggcggga atctccacct cttcctgcca cttctgcgcg gtggcacgaa tatcgccgta
    10981 atgacgtgcc ccggtcgcca tcagccgctc gccgatattg ccgcctgccg acacctggcg
    11041 gcgcaggccg tcgagtggat gctgatgcac cacgccccat tcggtacgca gcaggcgacg
    11101 gcggtcggcc tcgctcattg catacagcga acggttctcg tagtgaattt ccccctgctg
    11161 cggcgtcagg cgcgcggaga tcgacttcag cagcgtggtc ttcccggagc cggattcccc
    11221 gacaatgccc agcacttccc ccggccataa atcaaaagag acatcgctaa agcctttgcc
    11281 cggcgcgtaa aggtgggtca ggttattgac cgaaagtaac ggttgattca ttggtttttt
    11341 gcctcgctct gttggcggca ataatcggta tcggagcaga caaacatgcg gtttccggcg
    11401 tcatccagca ccacttcatc aagatagctg tgggtcgatc cgcagatggc gcatggctca
    11461 tcccactgct gaacggtgaa cgggtgatcg tcgaaatcga gactttccac gcgggtaaac
    11521 ggcggcaccg catagatgcg cttctcgcgc cccgcgccga acagttgcag ggcgggcatc
    11581 atgtccattt ttgggttatc gaatttcggg atcggcgacg ggtccatcac gtagcgccca
    11641 tttaccttca ccggataggc gtaggtggtg gcgatatgac cgaagcgggc gatatcttca
    11701 tacagtttca cctgcatcac gccgtactct tccagcgcgt gcatggtgcg ggtttccgtt
    11761 tcgcgcggct cgataaagcg cagcggctcg gggattggca cctggaagat aattatctga
    11821 tcttcggtca gcggcgtttc ggggatgcgg tgacgcgtct ggataaccgt cgcatcgtcc
    11881 gtacgttccg tggtgtttac cccggtcacg cgcttgaaga agttgcgaat cgacacggcg
    11941 ttggtggtgt catccgcgcc ctggtcaatc accttcagca cgtcgctttc gccaatcacg
    12001 ctggcggtaa gctgaatgcc gccggttccc cagccgtacg gcatcggcat ctcgcgcccg
    12061 ccaaacggca cctgataacc ggggatcgcc accgctttta agatggcgcg gcggatcatg
    12121 cgtttggtct gctcgtcgag gtaggcaaag ttgtagccgc tcagattagc catggttctg
    12181 ctcctgttgc agacgtttga gtagctccag ttcggcctgg aaatcgacgt agtgggggag
    12241 tttgaggtgt gagacaaagc ctgcggcttc gacgttgtcg gcatgtgcca gcacgaactc
    12301 ttcatcctgc gccgggcctg tcgcgtgctc gccgtactcc ggggcttgca gagcgcggtc
    12361 gaccagcgcc atcgccatcg ctttgcgctc gctcatgccg aacaccagcc cgtagccgcg
    12421 cgtgaagtgc ggcggctcac ccggcgggtc gataaaaccg ttaaccattt cacactcagt
    12481 catcagcagt tcgccgacgt tcaccgcaaa tcccagctct tccggcacaa tcgacacgtc
    12541 gatatagccg ctgcggatct cgcctgcgaa cgggtgattg cgcccgtaac cgcgttgggt
    12601 ggagtaggcc agcgccagca aatagccttc gtcgccgcgc atcaactgct gcaaacggga
    12661 ggagcgtgag caggggtaaa ccggcggcgt gcgggtgatg tcatccgcac gccgctatcc
    12721 tcttcaaact tcgccagccc ctgacgcgcc agcaggctga aaacgtgcgg cgacggctgc
    12781 tgttcgctgt cggcggtggt cagcgtcggc gcttcgccgt ttgccagcag ggtaaaatcg
    12841 agcaggcgat gggtgtagtc gtaggttggg ccaagcagct ggccgccggg aatgtcttta
    12901 taaacggcgg agatacggcg ttcgagacgc atcccggtgg tgtcgagcgg ctcgcttacc
    12961 gccagcttcg ccaacgtggt gcggtaggcg cgcagcagga aaatcgcttc aacgttatcg
    13021 ccgctggcct gtttcagcgc cagcgccgcc agttcgcggt cggcaatgcc gccttcggtc
    13081 atcacgcgat ctaccgcgag gttaagctgc tgttcaatct gggcgacgct cagttcgggc
    13141 aaatcggtat cgcctcggcg tcggctctct tgcagggcgt gggcggcgtc gatcgccttc
    13201 tcgccccctt tcacggcaac gtacatcagc acacctccac atgagtggtt cgcggaatag
    13261 ccagcaggcg ctcgccacag gtcaggatca ggtcgatgcc gagcgggaac ggatgcgggc
    13321 gctcggtgag ttcgtgcaga atgcactccg gcagctgcgg agcgatcatt cgttcttcgg
    13381 caatacccgc accagtaagg cgcaacatgc gtccgccgct caggctggcg acctgtaaaa
    13441 tcagcgtcgc acccgcttcc ggcgcaacgg cggtgccggt ggaaagggcg ttgagctgtt
    13501 cgctggaaat cgcctcatcc gtcaccgcga aggtcgcctg ttccggctgg ctgaccagcg
    13561 gcgcgttggt atgaaaacgc aggctctggt tgacgatatc gttatttaat ggggtagaaa
    13621 gccacaccgg cgtgtcgtta tcggccagcg tcagcagcac gctggtggtg gcgatattca
    13681 gcggttgcca gccgcgtttg agctgatgca gggcgacaat cacgcccggc tcgctcatgg
    13741 cctttaacag gcgacgaaaa ctgtgctggg catcctgcac gggaagcata aaagcggttt
    13801 ccagggtcat gcgttgtctc cgcgaaccat cgtaaagaag tcgacccggc tggcgttcac
    13861 ttcggcctgg cgtgcggcaa tgcgtgccat acggtcagcg tccagcgggg caataagggt
    13921 ttctgataag ttttgaaagt ggcgagattg ctgcatcagc gcgtcaatca gcgcgcagcg
    13981 ttcggcatgc tgtttatcac gcccctgcac ccagctgtag ccgagcgtgc cgtcagtcag
    14041 gcgcacggcg gcgcgggtca gcgtggcgtc gccggcaaaa aaacgttcgc cggtgccgcc
    14101 catccgcgcc tgaatctgta ccaggccagt ttcagcggcg cggatcacct catagtcggc
    14161 ggtgatgttt agcgcgttca ggcgtgctgc cagttcagcc ggttggctgt gcgccagcac
    14221 ggacatccag tgctggcggg tcgcggtatc tgcgtgcatt cagtgctcca tagtgaattc
    14281 aatcatgtcg gcgcgcgtca ggctgacgga gtactccgcc gggctgcttt caccgtcacg
    14341 gtggttaagg gtgcgcacgc acagcagcgg cgacatattc gggatttcaa gacgctggca
    14401 ctctttggcc tgggcgcggc gggcgctgat ccgcgtctgg ctgcggcgca gcgcaattcc
    14461 ggtttgctcg cgcagaaaat cgtgcagcga gccgctgtcg aagcgttgca gcgtcggcca
    14521 gagggtgagg tccgcgaagt agtggtcgat taaacagagc gcgacgccgt tgacacgacg
    14581 cagggtgcgc aggtggatga cgttctcccc ctcggtaatc cccagtgcgt cagcgacgtg
    14641 gccggacgcg gggcgcaata ccgaaagcag tttttcgctg gtgggatggc tgccctgatc
    14701 cagcagattc tggctaaaac gcgcctgggc gttgagcggg taatcgaacg ggcgcatcag
    14761 caccagcacg ccgacgccct gacggcgctg tacccagcct ttttccacca gttggtcgat
    14821 ggcgcggcgc agggtgtggc gattcacctc aaagcgcgct gccagttgct gctcggcggg
    14881 aagatagtcg ccgcagcggt agtgttgacg aagctcctgc tcaagttttg cggctatctc
    14941 ttgatagcgt gttgggtagc tggtcggatg tgtagacaag tgcatagata tcaatgcctc
    15001 gcttatcaga taaagtgctt acgcaaccgt tgagagagga aatccagcag gctgaccgtg
    15061 acgatgataa gcaccatcag ggcgcaggtt tgttggaact ggaaaccgcg aatcgcttcc
    15121 cacagggtga cgccgatccc gcctgcgccg accatgccga cgacggtcgc cgagcggacg
    15181 ttggattcga agcgatagag ggagtaggag atcagcagtg gcatcacctg tggcagcacg
    15241 ccgtagagga tctcttcgag cttgttggca ccggtggcgc gaatgccttc caccgggccg
    15301 ggttcaatcg cttccaccgc ttcggaaagc agcttggaga gcacgccggt ggtgtggata
    15361 aacagcgcca gcacgccagc acgccagcga acgggccgag gccgacggcg accacgaaca
    15421 gcatggcgaa gaccatttcg ttaatggcgc ggcaggcgtc catcaggcgg cgaacgggct
    15481 ggtaaaccca ccacggcacc aggttttcgg cgctcatcag gccaaagggg atggagagaa
    15541 ccaccgccag cgcggtgccc cagacggcga tttgcagcgt gacggccatt tcggtgaggt
    15601 aatcctgcca ctggctgaaa tcgggcggga agaagtcggc ggcgaacgtc gccatgttgc
    15661 cgccgtcttt gatcagcgta agcggggcca tttccgcgcc ctgccacgag acgaccaaca
    15721 cggagagtac aacggcccag ctcagaagcg agaaccagct gcgcttgggt ggggcgatgg
    15781 tgatggtttg catgtttggc tccggtctgt aggccggata aggtgcttgc accgcatccg
    15841 gcatcaacgc ctgcacattg tcggatgcga cgccggggcg tcttatccga cctacgattt
    15901 actgcaccgc tttactcacc gaactcatcg cgcttagcgc gttgttcagg cggtccaggt
    15961 catccagctg cgcctgaatc tcggtggttt ttgccagctt gtcctgctca ttcagtcctt
    16021 tattgctttt cacgccctgc atctctttaa acagcgcgag ctggcgaatc ggcaccagtt
    16081 gcaggtcgct ggaagcgcgg aatggcgccc agcccaggcg ttccagcacc gctttttctt
    16141 ccggcgtttt gccgtagttc ataaagaagt cgtagatctt gtctttggtg gtttcggaaa
    16201 gattcttgcg ccagacgatc ggatcgcctg ggatcagcgg cgacttccag atcaccttca
    16261 gttctttcag cttctctggc gcggaggttt tcagcttgtc gaggttttcg gtgttgttgg
    16321 tggcaacatc cacctgcttg ttggcgacgg ccagcgcgtt ggtttcatgc ccggcgttga
    16381 cggtgcgctt gaagtcgctg gcggagatat tgtttttggc gaagacgtag tagccgggga
    16441 cgaggaagcc agaggtggag ttaggatcgc cattgccaaa ggtgagatct ttccgcttcg
    16501 ccagcagatc gttcaggttg ttgatcggac tgtctttgtt gacgatcaac acgctccagt
    16561 aacccggcga tccatccgcc gcgaccgtct gggcgaagac ctggccattg gcgcgatcca
    16621 ccgcttccat cgccgacaga ttgccgtacc aggcgatatc cactttattg aagcgcatcc
    16681 cctggataat gcccgcgtag tccggggcaa agaaggcgtt gaccttcacg cccagcttct
    16741 tctccatatc ctgcaagaac ggcgtccatt gcggtttcag gttttgctgt gattccgttg
    16801 aaataatgcc gaaattcaac gccttttcct gctcttcggc gtgcgccggg cttaacaggg
    16861 tgctgaggct gaacatgctg gtgaaggcca gcgaggcaat tatcttagcg ttcattcgtt
    16921 ttcctcaatg atggggatgt caggcagctt tcgcgttctc ttcgacgcgg ttaatgctgc
    16981 ggtagagatg gtcaaaacgt tcgttatcaa actgttggct gctgccgtcg tagaagacgt
    17041 gcccctggcg cagggcgacg atgcgttcgc agtagcgcag ggcgtaatcc acctgatgca
    17101 gcgtgacgac cacggtgatg ccgtcgttct ggttgatgtc gcgcagggtg tccatcacga
    17161 tgcgcgctga ttctgggtcc agcgaggcga tgggttcatc ggccagaatc actttcgcct
    17221 gctgcatcag cgcacgggca atcgccacac gttgctgctg gccgccggag agggtggaaa
    17281 cgcgctgatg ggcaaaatgc accatgccaa cgcgggtcag cgcctgtaac gcgcgctgtt
    17341 tctgctcgcc ggtgaaccag ctaaaacagg tgcgccagaa cggcgtgctg ccgagcgcgc
    17401 caatcagcac gttctccagt acgctcaggc ggttcaccag gttgaattgt tggaatatgt
    17461 agccggtatg ggcgcggctt ttgcggatat cgcgggccag gcggccttcg cgctggactg
    17521 tgcggcccag cagctcgata tggctaccga cagatttatc gccggtaatc aaaccgctta
    17581 agtgacgtaa aagggtggat tttccggaac ccgacggccc aagcagagcc accatttcac
    17641 cgtgatgaat gttcagatca accgcatgca gcgcctgatg ctgattgaag gttttggcga
    17701 gcttctcgac acggataatc gtttgcatga tgcagcctcc ctaaaaaagt ggctccatcg
    17761 tggcggatta ttgtgacgat ttggttaatt aaaagtgact aacagatgaa gagttaacgg
    17821 gaattcgatg acagtgcggg agggcgggcc tcccggcggg ttattgcgtt ggttgttgtt
    17881 tgacgacatt aatcatccac ggtacgccaa atttatcggt gactttgcca aagccatgcg
    17941 cccagaagtt tcctgccagg ccatttcgat ttttccgtta gcggcaagat tgtcaaacca
    18001 gcgttttcct tcttcgacct gttgcgaatc gagcaccagc gtaaagccgg agtagctggc
    18061 ttttcctgac ggcatggcat cgctcatcat gatgtcgctt ccggcaatgc gcacattggc
    18121 gcgggcgatg gcggtatcgg gaaattgcat tccggaaggg cagttctcgg cgctgtcctg
    18181 cgctgatttt ggcatttcgc cgaagctgat tttatagagc agttccgcgc ccaacgtacg
    18241 ttgataatag gcaatcgcgt cggaacagtt accggcaaaa gagaggtagg gacttaacgg
    18301 catgatggtg acctcagtta agagaagcca gttaagtgta gttcgaaatt tatacagatg
    18361 agaggcggcc tgataagacg cgcgagcgtc gcatcaggca gtcggcactg ttgccggatg
    18421 cggcgtaaac gccttatccg gcctacggag ggtgcgggaa tttgtaggcc tgataagacg
    18481 cgcaagcgtc gcatcaggca gtcggcacca ttgccggatg cggcgtaaac gccttatccg
    18541 gcctacggag ggtgcgggaa tttgtaggcc tgataagacg cgcaagc
//
LOCUS       DBBGLQ010000085        18205 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.085, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000085 DBBGLQ010000000
VERSION     DBBGLQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.085"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(169..2094)
                     /gene="iolD"
                     /locus_tag="ACIYSB_003896"
     CDS             complement(169..2094)
                     /gene="iolD"
                     /locus_tag="ACIYSB_003896"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HGC5430840.1"
                     /translation="MQTERMTMAQALVRFLNQQYVSVDGKETPFVEGVATIFGHGNVL
                     GIGQALEQDPGHLQVMQGCNEQGIAHMATGFAKQHRRQRIFAVTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDIYACRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPE
                     QLMSAMLSAMRTLTDPANTGAVTICLPQDVQGEAWDYPLSFFARRVHHIERRPPDPQR
                     LAQAHALISRKRRPLVVCGGGVRYSGAHEALREFVEVLQLPFAETQAGKGALVSDHPL
                     NLGGVGVTGGMAANQLAPQADLVIGIGTRLTDFTTGSKALFSHPDVEFLLLNVAEFDS
                     LKLDATALIADARTGLTALTHALKDYRSGWGEAIAHAKSAWHDECCRLWERQWHPDDV
                     PEVAGHLDAQLMEYGETLNTHLTQTRVLGLINQHIEDNAIVVGAAGSLPGDLQRLWQV
                     KTPDSYHLEYGYSCMGYEVAAAIGAKLAKPQQPVYAMVGDGSYMMLHSELQTAVQEGI
                     KVTILLFDNASFGCINNLQMGHGMGSFGTENRYRNPKTGQLDGPLVKVDFAQNAESYG
                     CRAWRVHNEKSLLAALEASRAHPGPTLLDIKVLPKTMTHDYASWWRTGNAQVAESPAV
                     RQAAEQTQAQVKKARQY"
     gene            complement(2106..3611)
                     /locus_tag="ACIYSB_003897"
     CDS             complement(2106..3611)
                     /locus_tag="ACIYSB_003897"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGC5430841.1"
                     /translation="METVANFIHGECVTGSGQRIQAIFNPATGEQIRQVVMSTAQETE
                     QAIAAAQLAFPAWARFAPLKRARVMFRFKALLEENMERLARIISEEHGKVFSDAVGEL
                     TRGLEVVEFACGIPHLQKGEHSANVGTGVDSHSLMQPLGVCAGITPFNFPAMVPMWMF
                     PIALATGNTFVLKPSEKDPSLALALAKLLKEAGLPDGVFNVVQGDKESVDVLLTDPRV
                     QAVSFVGSTPIAEYMYLTASAHGKRCQALGGAKNHCILMPDADMDMATNAIMGAAYGA
                     AGERCMALSVVVAVGDKTADELCARLEKKIASLRVGPGLNQTPENEMGPLISAAHRSK
                     VLGYIDSGEAQGANLRVDGRGLRVKGHEQGYFVGPTLFDNVTPEMTIYKEEIFGPVLS
                     VVRVADYASAVDLINRHEYGNGSAIFTRDGGCARRFCEEVQAGMVGVNVPIPVPMAFH
                     CFGGWKRSIFGALNVHGNDGVRFYTRMKTITARWPEMQQETGAAFSMPTLG"
     gene            3902..4762
                     /locus_tag="ACIYSB_003898"
     CDS             3902..4762
                     /locus_tag="ACIYSB_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGC5430842.1"
                     /translation="MTTATSLNELQKQIRDRYDSLSKRLQQVSRYVLDNTNSVAFDTV
                     AVIAERADVPPSTLIRFANAFDFTGFNEMKQLFRMHLVEETASYSDRARLFREMESEA
                     VPETPLDILQEFASSNSQALQQLAARAEPEMLEHAVQLLAYADTIYIAGLRRSFSIAA
                     YLTYALSHLECRPILLDGMGGMLREQISRIKACDIVVSISFSPYAEETAMVSKTAASV
                     GARQIVITDSQISPLATFSDLCFVVKEAQVDAFRSQSATLCLVQSLVVALAYRLGDKK
                     HNNTQENSNQ"
     gene            complement(4958..5258)
                     /locus_tag="ACIYSB_003899"
                     /pseudo
     CDS             complement(4958..5258)
                     /locus_tag="ACIYSB_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5537..6331)
                     /locus_tag="ACIYSB_003900"
                     /pseudo
     CDS             complement(5537..6331)
                     /locus_tag="ACIYSB_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            6386..6562
                     /locus_tag="ACIYSB_003901"
                     /pseudo
     CDS             6386..6562
                     /locus_tag="ACIYSB_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6567..6821)
                     /locus_tag="ACIYSB_003902"
                     /pseudo
     CDS             complement(6567..6821)
                     /locus_tag="ACIYSB_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228719.1"
                     /note="participates in the regulation of osmotic pressure
                     changes within the cel; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
     gene            complement(6998..7930)
                     /gene="allS"
                     /locus_tag="ACIYSB_003903"
     CDS             complement(6998..7930)
                     /gene="allS"
                     /locus_tag="ACIYSB_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGC5430843.1"
                     /translation="MLDQETIRTFIQVAETGSFSRAASLLHKTPAAISYRIKTLEEQL
                     ATQLFLRTTRSVSLTLAGQHLLEHCRQWLNWLDVMPDELQQINAGVERQVNIVINNLL
                     YQPQSAADLLTWLHQQFPFTRFQLSRQVYMGVWDSLLYDDFQLAIGVTGSESLSNNIS
                     LLPLGDISWQFVVAQNHPLASHPSAVLSDDALRRYPAINIEDTSRTLTRRVAWLLDGQ
                     KEIKVPDLSTKLACHLSGLGVGFLPERMCRAYLESGALVARAVATPRQPSPLSVAWKN
                     AGSGKVVSEIARAFERKEALVSGFLRMVDRPAQA"
     gene            complement(7830..8720)
                     /locus_tag="ACIYSB_003904"
     CDS             complement(7830..8720)
                     /locus_tag="ACIYSB_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGC5430844.1"
                     /translation="MKTLNALACNGLEQLADGVWQRHSRFSQAVNFVHADGTLLTLFR
                     YGKGMGPTGILLSSTQFAQLANLPRLVKQGRLLWGAGVAIRPRRTLKLRFPVGHSFPL
                     DLSAYSRQSGLRVALNQPLADMPFYSSIMGELERWRRGDLPDWGWLIGNGPGLTPSGD
                     DMLAGMLAMLYGAGFSDRLHAFIPPADQLTSLTTSVSCSYLNSARRGEFSLPVVKVMR
                     GLRSTGDPKRAIQRLLAVGHTSGADTLLGMAFAQHWLQRVDSRGTHARSGNHPHVYTG
                     RRDREFLPRGQSAAQNAGGD"
     gene            complement(8761..10023)
                     /locus_tag="ACIYSB_003905"
     CDS             complement(8761..10023)
                     /locus_tag="ACIYSB_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGC5430845.1"
                     /translation="MYSSIAQANEAIIERIKAARPHWVAVRPAKEAVAELSSGRKLLH
                     AGPPIAWQEMTGPMRGACIGASLFEGWATSEEEAVRMLEQGEVEFIPCHHVGAVGPMG
                     GITSANMPVLVVRDMVHGNQSCCNLNEGIGKVMRFGAYGEDVQTRLRWMRDTLAPVLQ
                     EALSLMENGVDLTAMMAQAITMGDEFHQRNIAASSLLLRTLSPVIAGLDRPNAEIVAV
                     LQFLSVTDQFFLNLAMAYSKAVMDAAAEIKAGSVVTAMTRNGREFGIRVSGTGDRWFT
                     APVNTPQGLFFTGYSQADANPDIGDSAITETLGIGGAAMIAAPGVTRFVGAGGMGAAL
                     ETSEEMSEIYLANNPLFQIPSWDFKGACLGLDVRRVVETGITPLINTGIAHREAGIGQ
                     VGAGTVRAPLLCFEKALEALAELHHITA"
     gene            complement(10040..11701)
                     /locus_tag="ACIYSB_003906"
     CDS             complement(10040..11701)
                     /locus_tag="ACIYSB_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGC5430846.1"
                     /translation="MIYAFIKKGSFQDSVSLMIISRKLSERPEVDQVSVMMGTPANKS
                     MLESTGFWHPDFAEATPNDICVAVRTQEAQPEILDLIREQLEKELSAIANASGSSTQL
                     VKARRWESACQKLPDANLLLVSVAGEYAAGVAKEGLLANKSVMLFSDNVPLEQEVELK
                     TLAREKGLIVMGPDCGTAMIAGSPLAFANVLPQGGIGVIGASGTGIQEITSQVALHQQ
                     GISHAIGLGGRDLSAEVGGISALTALEMLAADSATQVIAFVSKPPSPQVRARIIAAMQ
                     KQNKPVVALFLGSRAEQRREGNVWLANSLADAAQLAVLLMRVAQQRQSRPQVAGKGIY
                     GLYAGGTLAAEAAMLLSAHLGVPVSDSHADGVMLEAGGHRIVDLGDDSYTLGRPHPMI
                     DPTTRSIEIEKLAAMPEVGVLLLDVVLGYGACADPAGAVVEAIEQVRRKRVAPLVVIA
                     TMTGTDADPQGRSEQIAILGNAGVAVVETLEEATLLAVSLTQHQPQSESTAHNPLLDG
                     VQVINAGLRSFALDLQSSGTPVVHYQWAPVAGGNARLASLLKQLH"
     gene            complement(11715..12938)
                     /locus_tag="ACIYSB_003907"
     CDS             complement(11715..12938)
                     /locus_tag="ACIYSB_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5430847.1"
                     /translation="MSGTYEVTEVTKRKGIPAWWVMVFLFWLGWVFMYADRTILNPVM
                     GELEKEFGLSGTQLGVLNSVFYFSYALLQVPAGILGDKIGKKKVLVPGFILFGVFTAV
                     TGWVKSWYMLLFARVVTGAGEGTYYGPQYGLSSEQIPKKFRSLGSAIINSGMAFGIAL
                     GLMTSSWLVYDQGYSWRMPFYAMAIPSVLVGVAIWLFVKEKKRTADAEGKPVQKGKFS
                     DLLKNRNLILTYIMVFCSLFGFFVILTWLPYYLQNERGIAGSETGFISSLVAWISIPG
                     ALLFSGLSDKLGRRKPLILFLVPVAIASMLSIIWMPSMTGVIVALCVYGLVGKLALDP
                     VLVALVADSVDETNYSTAFGLFNFIGMSSSILAPIIAGAARDITGSLASSFYVSAALL
                     VIGLVAMLFLKEKRT"
     gene            13266..14168
                     /locus_tag="ACIYSB_003908"
     CDS             13266..14168
                     /locus_tag="ACIYSB_003908"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGC5430848.1"
                     /translation="MKKKIVIALGGNALLQRGEVLSAENQQRSIQLFARMAAELARDY
                     QLVIVHGNGPQVGLLALQNAAYQESPAWPLDILVAESQGMIGFAIAQELMQYAGAPVS
                     TLMTRVEVAANDDAFAAPGKYIGPVYPPQQQAELEKRYGWQMKADGDYIRRVVPSPRP
                     LNIVDSAAIQTLMDAGHLVICCGGGGIPVVADTHGYRGVEAVIDKDLTAAALASAIHA
                     DHLLILTDADAVYVDWGTPQARPLHHATIATLAPFAAPDGAMGPKAEAVIQFVKQTGN
                     NAFIGALKDAPQILAGEKGTCVSR"
     gene            complement(14204..15341)
                     /locus_tag="ACIYSB_003909"
                     /pseudo
     CDS             complement(14204..15341)
                     /locus_tag="ACIYSB_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            15530..15760
                     /locus_tag="ACIYSB_003910"
     CDS             15530..15760
                     /locus_tag="ACIYSB_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGC5430849.1"
                     /translation="MPFKSLRQWPQDVAKAEKRARAPEIIPVSVSGTPGMPDGRPLLD
                     EPVTLFLPGGIRMCCQPSQLTDVFRALRHADA"
     gene            15747..16127
                     /gene="tnpB"
                     /locus_tag="ACIYSB_003911"
     CDS             15747..16127
                     /gene="tnpB"
                     /locus_tag="ACIYSB_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837255.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGC5430850.1"
                     /translation="MLTPDHVFLVVSPVDMRRGIDTLTQYVQHELNAAWHDGAAFVFT
                     NKTRSRIKVLRWDKHGVWLCTRRLHRGTFRWPRKGDATWHLTPDEFHWLVCGVDWQQV
                     KGHDLAKWVYQEKTADAQSNAITQ"
     gene            16170..17754
                     /locus_tag="ACIYSB_003912"
                     /pseudo
     CDS             16170..17754
                     /locus_tag="ACIYSB_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         4191 a         5236 c         4728 g         4050 t
ORIGIN      
        1 gtattagata aattttgtta tcaatctcac aacgccgagg aagatatgtc cctccattac
       61 aaggggggga gggaataaaa actgtaccat cccctctccc gttgggagag gggatgggtg
      121 aggggaaaaa agaaaaatga aatgaaaatt tcgttacgac aggaaaactc aatactgacg
      181 agcttttttc acctgagcct gtgtttgctc ggcggcctgg cgcactgctg gcgactctgc
      241 cacctgtgcg ttgccggttc gccaccatga cgcgtaatca tgggtcatgg tttttggtag
      301 cactttgatg tcgaggagcg tcggtcccgg gtgagcgcgc gaggcttcca gcgccgccag
      361 caggcttttc tcattatgta cccgccatgc gcggcaaccg tagctttccg cgttttgcgc
      421 aaaatcgacc ttcaccaacg gcccgtcaag ctgacctgtt ttcggattac ggtaacgatt
      481 ttctgtgcca aaactcccca ttccgtgccc catctgcagg ttattaatgc agccgaagct
      541 ggcgttatca aacagcagaa tggtgacctt aattccctcc tgtacggcag tttgcagctc
      601 tgagtgcagc atcatgtacg aaccatcgcc taccatggca tacaccggct gctgaggttt
      661 tgccagcttc gcaccaatag ccgctgcgac ctcatacccc atgcaggagt aaccatactc
      721 cagatggtaa ctgtcaggcg ttttaacctg ccagaggcgt tgcaaatccc ctggcagcga
      781 tccggccgcc cccaccacaa tggcgttatc ctcaatatgc tggttaatga gccccaacac
      841 ccgcgtctgg gtcaggtgtg tgttgagcgt ttcaccgtat tccattagct gtgcatcaag
      901 gtgccctgcc acttcgggaa cgtcgtcagg atgccactgg cgttcccaca gtcgacagca
      961 ttcatcgtgc caagcggatt tcgcatgggc aatcgcctcc ccccagccac tacgataatc
     1021 tttcagtgcg tgggtcaacg ctgtcagacc agttcgggca tcggcaatga gcgcggtggc
     1081 atccagcttc agggagtcaa actcagccac gttcagcagc aggaactcga cgtcgggatg
     1141 agaaaagagc gccttagagc cggtagtaaa atccgtcagg cgcgtgccga tgccaatcac
     1201 taagtcagcc tgcggcgcaa gctggttggc agccattccg cccgtcacgc ccaccccgcc
     1261 cagattaagc gggtggtcac ttaccagcgc ccccttaccg gcctgggttt cggcaaacgg
     1321 caattgcagc acttcaacaa attcccgtaa cgcctcatgc gcaccggagt aacgcacgcc
     1381 accgccgcac accaccagtg gacggcgctt gcgggaaatc aatgcatgag cctgcgccag
     1441 tcgctgcggg tcaggcggac ggcgttcaat atgatgcacc cgacgcgcaa agaaactcag
     1501 ggggtaatcc caggcctccc cctgcacatc ctgaggtagg caaatggtca ccgctcctgt
     1561 atttgcgggg tcagtaagcg ttcgcatcgc acttaacatc gcgctcataa gctgttcagg
     1621 acggttgatg cggtcccagt agcgggaaac cgggcggaag caatcgttgg tactgatgct
     1681 gagatcgtga tactgctcga tctgctgcaa cacggggtca ggctggcggc aggcgtagat
     1741 atcccctggc agtagcagca gtggaatgcg gttcgccgtg gccgtcgctg ccgccgtcac
     1801 catattggca gcgccaggcc caacggatga ggtgacagca aaaatacgct gacggcgatg
     1861 ctgtttggcg aatcccgtcg ccatatgggc aatcccctgc tcgttacagc cttgcatcac
     1921 ctgcagatga ccaggatcct gctccagcgc ctggccgata ccgagcacgt taccgtgccc
     1981 aaagatcgtt gccacgccct ccacaaaggg cgtttcttta ccatcaacgc taacatattg
     2041 ttggttaaga aatctgacca gcgcctgggc catagtcata cgttcggttt gcataaaacc
     2101 ccctgtcagc ccagcgtcgg catggagaac gctgcacctg tctcctgctg catttccggc
     2161 cagcgtgcag tgatggtttt cattcgggtg tagaaacgca cgccatcgtt cccgtgaaca
     2221 ttgagcgcgc caaaaatgga acgtttccag ccaccgaaac agtggaatgc cattggcacc
     2281 gggatcggca cgttgacccc caccataccc gcctgcacct cttcacaaaa gcggcgtgcg
     2341 cagccaccgt cacgggtgaa aatggccgag ccattaccat attcgtgtcg gttaatcaga
     2401 tccaccgcgc tggcgtaatc tgctacgcgc accacagaaa gcaccggtcc gaaaatttct
     2461 tctttataga tggtcatctc cggggtgacg ttatcgaaca gagtcggccc aacgaaatag
     2521 ccctgctcgt gccctttcac tctgaggcca cgcccgtcca cacgcaggtt agccccctga
     2581 gcttcgccac tgtcgatata gcccaacact ttgctgcggt gtgcggcgga gatcagcggc
     2641 cccatctcgt tttctggcgt ctggttcagg ccaggtccga cgcgcaacga agcgatcttt
     2701 ttttccagcc gggcgcagag ttcatcagcc gttttatcgc caaccgccac caccacggaa
     2761 agcgccatgc agcgttctcc tgccgcacca taagctgcgc ccattatggc gtttgtcgcc
     2821 atatccatat cggcatccgg cattaaaatg cagtggtttt tcgcaccgcc gagcgcctga
     2881 cagcgcttac cgtgggcgga cgcagtcagg tacatgtatt cggcaatcgg tgttgaaccg
     2941 acaaaactga cggcctgcac gcgcgggtcg gtcaggagca catcaacgga ttctttatcg
     3001 ccctgcacca cgttgaatac gccgtccggc aggcctgcct ctttcagcag tttcgccagc
     3061 gcgagcgcca gagacggatc tttttccgac ggtttcagca cgaaggtgtt gcctgtcgcc
     3121 agagctatcg ggaacatcca cattggcacc atcgccggga agttaaacgg cgtgatcccg
     3181 gcgcagaccc cgagcggctg catcagtgag tggctgtcga cacctgtgcc aacgttcgca
     3241 gagtgttcgc ctttttgcag atgtgggatg ccgcaggcga attctaccac ttccagacca
     3301 cgggtcagtt cacctactgc atctgagaag accttaccgt gttcttcact gataatacgg
     3361 gccagacgct ccatattctc ttcaagcaac gctttgaagc ggaacatcac acgcgcgcgc
     3421 ttgagtggag caaaacgtgc ccacgcggga aacgccaact gtgctgcggc aatcgcctgt
     3481 tcggtttcct gtgccgtact catcaccacc tggcgaatct gctcgccagt ggccgggttg
     3541 aagatcgcct gaatgcgctg gcctgaaccg gtgacacatt cgccgtgaat aaaattcgct
     3601 accgtctcca tccttaacct ctcgctgttg atacgtgatt gttacctgaa cgttatgtac
     3661 tgaacactat atatgaaaca aatattccat tttaagttga aataaaataa ataatgatta
     3721 ttgtgatcaa ggatggattt ttatgtgaac agccagcatc actgactcag cctgagctat
     3781 taggagtttc gggcgaggaa atgactgaag cttctgtttc atttttgctg ccaagtggat
     3841 ggccaacatt taaaagacgc ggttttgcgt ttagaatcat gagactggat agggggataa
     3901 catgacgaca gcaaccagcc tgaacgaact acaaaagcaa atccgcgatc gctacgatag
     3961 cctgagcaaa aggctgcagc aggtctcccg ctatgtactg gataatacga acagtgtcgc
     4021 gtttgatacg gttgcggtca tagccgaacg tgccgatgtt ccaccctcta cactgattcg
     4081 ttttgccaat gcctttgact tcaccggctt caacgaaatg aaacagttat ttcgtatgca
     4141 cctggtggag gaaaccgcca gctacagcga tcgtgcgcga ctgttccgcg aaatggagag
     4201 cgaagccgta cctgaaacgc cgctcgatat tttgcaggag ttcgccagtt cgaactcgca
     4261 ggcgctgcag cagttggctg cccgcgccga accggagatg ctggagcacg ccgtacaact
     4321 actggcctac gccgacacca tttacatcgc cggattacgc agatccttta gcattgctgc
     4381 ttatcttacc tatgctttaa gccacctaga gtgccgccct attctgcttg acgggatggg
     4441 cggcatgctg cgcgaacaga tcagccgtat taaggcgtgc gacattgtgg tgtcgatcag
     4501 tttctcgccg tacgcggaag aaacggcgat ggtcagcaaa accgccgcca gcgtcggcgc
     4561 tcggcagatt gtgattacgg atagccagat cagcccactg gcaacgttca gtgatctctg
     4621 ctttgtggtg aaagaggctc aggtggacgc atttcggtct cagtcggcaa cattgtgtct
     4681 ggtgcagtcg ttagtggtcg cgctagctta ccgtttagga gataaaaaac ataataatac
     4741 ccaggaaaac agtaaccaat aataaccaat gaactcctcc cccgagtcca tataagagta
     4801 aatgattcgg tgccagtagc gatacagact tcagattgaa tatcaactgt actgctatct
     4861 ccctaaaagg tcatgcccca tgtgagcgta caacgagagc cttaatcctt aatagcgaag
     4921 aagatttctc tagtaggtgg taatgtaacc cagatataac ggcttcttta aacagtttat
     4981 ttaccatcgt tttcttacct agcaggcgct gaagttcttt aattttattc atggcggcag
     5041 caagttcaga ggccggaata cccttttctt cggctgtcac agtagtgaag cttccttcaa
     5101 ggtaatgata cgccagagaa ataacgggct gaccaccata ccatgttgcc aggcaatcag
     5161 gaagaccatc attcctggtt caaaactctg ttgaacagta gcgattttgc gtgtgtgatg
     5221 cgcagtctgc gtttctccga tcctgagaca tcaatcatct gttctatatg aacgcatccc
     5281 gtttgcaagg ctttcgtagt tttgacattg acggtaggtt gcagctctat atccggcctt
     5341 gttgcagcca tgaagctgcg ggcccctatg taatacgctg actcgcgcct tattctccca
     5401 gcgagcttta atctccagaa cagattcagg tttagcgagt cccggtctta cctgtcgttg
     5461 tcatcactac ggctgcccac gcaacctttc agcattcacc tttttgtaaa gagtttatgt
     5521 ttatcggtat cagcgattaa taaggcctga tactgacatg atttttgtcg tatttacgtc
     5581 cgtgagcggc tattgcccac accgttcgcg ccagcttgtt tgccattgcg acgattgcca
     5641 cgctggccgg acgacgtttt ttcagttcgg ttatccacgg cccaggttct ttggtcagta
     5701 atgctgccgc ccttgcacca tgaataaaca gggttcgcag atatttatca ccacgtttgc
     5761 ttattcccag caggcggatt tttcctcctg agcctgtctg tctgggcacc agaccaacat
     5821 aagccgcgaa ctctcgccct gatttaaaca ctgatgcttt ccccatgatg gccaacgctg
     5881 ccgttgcaat aagtggccca acgccaggaa tatccagcaa ccgtctacag gtttcattct
     5941 gtctcgccac actggtaagc tgtttttcta tatcatctat cagtgagtcc agttcattca
     6001 gcctgtagta ctgattttcc agcactgtaa tgagatacag tggcagtctc tctttcatcc
     6061 gctccagtgc ttcagggaac tcccgttcca tcgctgcccg acctttgtgg atggtttcac
     6121 caaactccag taacgtcccg tgcagggcat taatttgcgc ggtccggaac ttcaccagtt
     6181 gtctgcggga acggtgcaga accagtattg actgttgttc ttctgttttg acggcgattt
     6241 ctttgcctgg ctgctgtacc gccatccaga tagctcgcgc atccatcacg tcattcttat
     6301 tgcccatgac gaatgattta acgaagcgag ctgaaccgcc ccgggaatcc tggagactaa
     6361 actccctgag aaagaggtaa acagaatgac taaaaatact cgtttttccc ccgaagtccg
     6421 tcagagggcg attcgtatgg ttctggaaag tcagggcgaa tatgactcac aatgggcgac
     6481 aatttgttcc attgctccaa agattggctg tacgccggag actctgcgtg tctgggttcg
     6541 ccagcatgag cgggataccg ggggcggccc agcacagcaa tcactgaggc ggtctgcacg
     6601 ccaacgcggc ccagagcggc aatcagcgta aaggcgataa tcgcatagcg caccagcgca
     6661 gagagaaaat cggcgacagt cgcgtcgatc ttacgcgcca gcatcaggcg gttgacggtg
     6721 ttagaaacga tgcgcgccac gatcatcccg atgatcagga taacgatagc ggcaacgatg
     6781 ttgacggcat agctcagcag caactcctgg ttgcgcgcca gctaggtccc ggcatggttg
     6841 atgctatcga caacgttcaa atcttccatt tagtaatcct tgggtcatat caaaaaaagc
     6901 aaatgagatc attctcacga aaatgacctt ttaaaggtaa acaaaaacca ccgaagatgc
     6961 catttctcaa tctgatccag atgaaactaa cgccgcatta cgcttgcgca ggacgatcca
     7021 ccatccgcaa aaaaccgctg actaatgcct ctttgcgctc gaacgccctg gcgatttcac
     7081 tgacgacttt accgctgccg gcattcttcc aggcaacgga gagcggcgaa ggctggcggg
     7141 gggtggcgac cgcacgggcc accagcgcac cggattccag gtaagcgcgg cacattcgtt
     7201 ccggcaaaaa accgaccccc aggccgctta gatggcaggc cagcttggtg ctgagatccg
     7261 gcaccttgat ctctttttgc ccgtcgagca gccaggcgac acgacgcgtc agagtccgcg
     7321 acgtatcctc aatgttaatt gccggatagc ggcgtagcgc gtcgtcggag agtaccgccg
     7381 aggggtgact ggcaagggga tgattctgcg ccaccacaaa ctgccagcta atatcgccga
     7441 gcggcaataa agagatattg ttggacaggg attccgagcc ggttacgcca atcgccagct
     7501 ggaaatcgtc gtatagcaaa gagtcccaga cccccatata gacctggcgg gaaagctgaa
     7561 agcgggtaaa aggaaactgc tgatgtagcc aagtcaggag atctgcggca ctttgcggct
     7621 gatacagaag attattgata acgatattaa cctgtctttc gacgccggca ttaatctgct
     7681 ggagttcatc gggcatcacg tccagccagt tcagccactg ccgacagtgc tcaagcagat
     7741 gttgcccggc cagcgtcagg cttaccgagc gggttgtgcg taagaagagc tgcgttgcca
     7801 actgctcctc cagcgttttg atacgatagc taatcgccgc cggcgttttg tgcagcagac
     7861 tggccgcgcg ggagaaactc ccggtctcgg cgacctgtat aaacgtgcgg atggtttcct
     7921 gatcgagcat gcgttcccct tgaatccact ctctgtaacc agtgctgcgc gaatgccatc
     7981 cccagcaggg tatcagcccc tgaagtatga cctaccgcca gcaaacgctg aattgcccgc
     8041 tttggatcgc ctgtcgaccg cagcccgcgc attaccttaa ccaccggcag cgaaaactcg
     8101 ccgcgcctgg cgctattcag atagctacag cttaccgaag ttgtcagcga ggtaagttga
     8161 tctgccgggg ggataaacgc gtgcaggcgg tcagaaaaac cggcgccgta cagcatcgcc
     8221 aacatcccgg caagcatatc atcgccgctg ggggttaatc ccggcccgtt gccaatcagc
     8281 catccccagt ccgggagatc gccgcgccgc cagcgctcaa gctcgcccat aatagatgaa
     8341 taaaacggca tatccgctaa cggttgatta agcgcgacgc gaagtccgct ctgacgtgaa
     8401 taagccgaaa gatcgagagg aaacgagtgg ccgacgggaa agcgaagttt gagcgtccga
     8461 cgcgggcgga tggcgactcc cgccccccat aataagcgcc cttgcttaac cagccgcgga
     8521 agattagcca actgcgcaaa ctgtgtgctg ctaagcagaa tccccgtcgg acccatacct
     8581 ttgccgtagc gaaacaacgt cagtaaagtc ccatcggcgt ggacgaaatt aaccgcctgt
     8641 gaaaaccggc tatgacgctg ccacacgcca tcggctaact gttcaaggcc gttacaggcc
     8701 agcgcattga gggttttcat cggcatctct ccggggcggc agcaccgccc ctctctcgac
     8761 tcaggcagta atgtggtgca gttccgctaa cgcttccagc gctttctcaa agcacagcag
     8821 cggtgcgcga acggttcccg ctcccacctg gcctatccct gcttcgcggt gggcaatacc
     8881 ggtattgata agcggcgtga tgccggtttc cacgacccga cgaacatcca gccccaggca
     8941 ggcgcctttg aaatcccatg atgggatctg gaacagcggg ttgttggcaa gatagatttc
     9001 agacatctct tccgatgttt ccagcgccgc gcccattccg ccagcgccga cgaaccgcgt
     9061 cacgcccggt gcggcaatca tcgccgcacc gccgatcccc agcgtttcgg taatcgcgct
     9121 atcgccgatg tccgggttag catccgcctg gctgtagccg gtgaagaaca gcccctgcgg
     9181 cgtattcacc ggtgcggtaa accagcgatc gccggtaccg ctaacgcgaa tgccgaactc
     9241 gcggccattg cgggtcatgg cggtcaccac cgagccggct ttaatttccg ccgccgcatc
     9301 catcaccgct ttgctgtaag ccatcgccag gttgaggaag aactgatcgg tgacgctgag
     9361 gaactgcagc acggcgacaa tttccgcatt cggccgatcc aggccggcaa tgaccggcga
     9421 tagcgtgcgc agcagcagcg acgaggcggc gatattacgc tggtggaact catcgcccat
     9481 ggtaatcgcc tgcgccatca tcgccgtcag gtcaacgccg ttttccatca gcgacagcgc
     9541 ctcctgcagc accggcgcga gcgtgtcgcg catccagcgc agacgcgttt gcacatcctc
     9601 gccgtaggcg ccgaagcgca tcactttgcc gatcccttcg ttcaggttgc agcaggattg
     9661 gttgccatga accatatcgc gcaccaccag caccggcatg ttcgccgagg tgatcccccc
     9721 catcggcccc accgcgccca cgtggtgaca agggatgaat tccacctctc cctgctccag
     9781 catccgcacg gcttcttctt cgctcgtcgc ccagccttca aacaacgaag cgccgataca
     9841 ggctccgcgc atcggcccgg tcatctcttg ccaggcgatt ggcggcccgg cgtgcagtaa
     9901 tttacgtcca gaagataatt cagccaccgc ctctttcgca ggacggacgg caacccaatg
     9961 cgggcgcgcg gctttaattc gttcgataat ggcttcgtta gcctgtgcaa ttgatgagta
    10021 catatccata cctctgaact taatgtaact gcttgagtaa actggcaaga cgagcgttac
    10081 cgccggcaac gggcgcccac tgataatgca ctaccggcgt accgctgctt tgtagatcca
    10141 gcgcgaagct gcgcaacccg gcattaatga cctgcacgcc gtcgagcagc gggttatgcg
    10201 ctgtcgattc gctctgcggc tgatgctgcg tcaggctaac cgccagtagc gtcgcctctt
    10261 cgagggtttc aaccaccgcg accccggcat tgcccaggat ggctatctgc tcgctacggc
    10321 cctgcgggtc ggcatcggta ccggtcatgg tggcgataac taccaacggc gcgacacgtt
    10381 tacggcgaac ctgctcgatg gcctcaacca ctgccccggc aggatcggcg caggcgccat
    10441 agcccagcac cacatccagc aacagcacgc caacttccgg catcgccgcc agcttctcaa
    10501 tttcaatgct gcgggtggtg gggtcgatca tcgggtgcgg tctgccgagg gtgtagctat
    10561 cgtcgccaag gtcgacaatg cgatgcccgc cggcttccag catcacaccg tcggcgtggc
    10621 tgtcgctgac cggcactccc agatgagcgg aaagcagcat cgcggcctcg gcggcaaggg
    10681 taccgccagc gtacagacca taaatcccct tgcctgcaac ctgcggtcgg ctctgccgct
    10741 gttgcgctac gcgcatcagc agtaccgcca gttgcgcggc gtccgccagc gagttcgcca
    10801 gccagacgtt gccctcgcgg cgctgctcgg cacggctgcc gaggaacagc gccaccaccg
    10861 gtttattctg tttttgcatg gcggcaatga tgcgggcacg cacctgtggc gaaggcggtt
    10921 tagagacaaa agcgataacc tgcgtcgcgc tatcggccgc cagcatctcc agcgcggtca
    10981 gggcgctgat accgccgact tcggcgctga gatcgcgccc gccaaggccg atggcgtggc
    11041 taattccctg ttgatgcagc gccacctgcg aggtgatttc ctggatgccg gtccccgatg
    11101 cgccgatgac gccaattccg ccctgcggca aaacgttagc gaaagccagt ggactaccgg
    11161 cgatcatcgc cgtcccgcag tccggaccca tcacaatgag ccccttctcg cgagccagcg
    11221 ttttcagttc aacttcttgc tccagcggga cgttatcgga aaacagcatc acgctcttat
    11281 tggctaacaa tccctctttc gccaccccgg cggcgtactc gcctgccacc gaaaccagca
    11341 acagattcgc gtccggtaat ttctggcaag cgctctccca gcgccgcgcc ttgaccagct
    11401 gcgttgagct gcccgatgca ttagcgatag cgctcagctc tttctctaat tgttcacgga
    11461 ttaaatcgag aatttccggc tgcgcttcct gcgtgcggac tgcgacacaa atatcgttgg
    11521 gcgtcgcctc ggcgaaatcc gggtgccaga atccggtaga ctccagcatt gatttattcg
    11581 ccggggtgcc catcatgacc gaaacctgat ccacttccgg gcgttcactt aatttgcggg
    11641 aaataatcat caggcttacg gaatcctgaa agcttccctt tttaataaaa gcgtaaatca
    11701 ttaccgctac ctctttatgt acgtttttct tttaaaaaca gcatggcgac cagaccaatg
    11761 accagcagcg ccgcggaaac ataaaaactc gaagccagac tgccggtaat atcacgcgcc
    11821 gcccctgcaa taattggcgc cagaatcgat gagctcatcc cgataaaatt aaataaacca
    11881 aacgcggtac tgtaattggt ctcatcgacg ctatcggcga ccagcgccac cagcaccgga
    11941 tccagcgcca gtttgccaac cagaccgtac acgcacagcg ccacgatgac gccggtcata
    12001 ctcggcatcc aaataatgga aagcatgctg gcaatcgcca ccggcacgag gaataaaatt
    12061 aacggtttgc ggcggcctaa tttatccgac agccccgaga acaacagcgc gccgggaata
    12121 gagatccacg cgaccagcga cgaaataaac ccagtttcgc taccggcaat accgcgctcg
    12181 ttttgcagat aataaggaag ccaggtcaga ataacgaaga agccaaacag gctgcagaac
    12241 accatgatat aggtcagaat taaattacga ttcttcaaca aatcgctaaa tttgcctttt
    12301 tgtaccggct tgccctcggc gtcggcggtg cgttttttct ctttcacaaa gagccagata
    12361 gcaaccccta ccagtaccga aggtatcgcc atggcataaa atggcatccg ccaggagtaa
    12421 ccctggtcat aaaccagcca actggaggtc atcagaccca acgcgatgcc aaaggccatt
    12481 ccgctattga taatcgcgct acccagcgag cggaactttt tcgggatctg ctcggaagac
    12541 aatccgtact gcgggccata atatgtcccc tcgcctgcgc cggtgaccac gcgggcaaac
    12601 agcagcatat accagctttt tacccaaccg gtgaccgcgg taaaaacgcc aaacagaata
    12661 aacccaggca ccagcacttt tttcttaccg attttgtccc cgaggatccc cgccggcacc
    12721 tgcagcaatg cataagaaaa ataaaatacg gagttgagca ctcctaattg cgtaccgctt
    12781 aaaccaaatt ctttttccag ttcgcccatc accggattta atatcgtgcg atcggcatac
    12841 ataaataccc aacctaacca gaaaagaaat accatgaccc accaggcggg tattcctttt
    12901 cttttggtca cctcggttac ctcatacgtt cctgacataa gctatctccc ggcaatattc
    12961 acctcaggcc atcccagcgg atgggtgaag gattgatatt ttaactctgc taaccatatc
    13021 ggggtaacca tgtattttat ttgaacaaag tcacaaaaaa tccattgctt taaattccta
    13081 aagactgctg acgaaattat taaaaaaaat ctaataatca tctttaaaaa gatttaaata
    13141 aaaccagcga atataatatt tattagcgag atatttttta ctcttgataa agtggcgatc
    13201 tcatcacatt acctggccgc taaaacgata tttttagcac acttctataa ttcaatgagg
    13261 tcatcatgaa aaagaaaatc gtcattgcgc tgggtggtaa cgcactgctg cagcgcggag
    13321 aagtactcag cgcggaaaat cagcagcgca gcattcaact ttttgcccgg atggcggcgg
    13381 aattagcccg cgattaccaa ctggtgatcg tacacggcaa tggcccgcag gtcggattgc
    13441 tggcgctgca aaacgccgcc tatcaggaat ctcccgcctg gccgctggat attctggtcg
    13501 cggaaagtca ggggatgata ggcttcgcta tcgctcagga actgatgcag tacgccggtg
    13561 cgccggtcag caccctgatg acacgcgtag aggtggcggc aaatgatgac gctttcgcgg
    13621 caccggggaa atatattggc ccggtttatc cgccgcagca gcaggcggag ctggaaaaac
    13681 gctatggttg gcagatgaaa gcggatggcg actatatccg tcgggtggtc ccttccccca
    13741 gaccgctaaa tattgttgat agcgcggcca ttcagacgct gatggatgcc ggtcatctgg
    13801 tgatttgctg cggcggaggc gggatcccgg tggttgccga tacgcacggt taccggggag
    13861 tcgaagcggt tatcgataaa gatctgacgg cggcggcgct tgccagtgcc attcacgccg
    13921 accacctgct gattctgacg gatgccgatg cggtctatgt tgactggggg acgccgcagg
    13981 ctcgcccgtt gcatcacgcc accattgcga cgctcgcgcc atttgccgcg ccggacggcg
    14041 caatggggcc gaaagcggaa gcggtcattc agtttgtcaa acagaccgga aataacgcct
    14101 ttatcggcgc gttaaaggat gcgccacaga tattagccgg ggagaaaggg acctgtgtaa
    14161 gccgctaaaa aaaatggctt cccgcaggaa taatgccagt cagttaagca actgactggc
    14221 tctttttcgg ggctgtgggg tatttccagg gcctctcctt taccactctc gggaaggccc
    14281 tttcccttct tgtcggtaat ttcactggtt gtcccatact tgcaagatcg cgcatcagct
    14341 ccggtatacg tcccggtgaa gcgccctgca atgtcatcag cattctcatc accattccgc
    14401 atgattctga gaaactcagt tgattcggcc agtaaccttt cagatgttcc gccattttaa
    14461 tcatctgata tctcaccaga ttataagcca gtaagacccc ccacagctct tgctccacaa
    14521 gctccggctt tttacttctc agcgtcagcc tgctcagttg catcgtctgt tttatctccc
    14581 tgtatcccag ttcgatttcc cagcgatgac tgtacagatc cgccatttct cctccgggga
    14641 agcgcatggc gtccgtcatc gacgtcagca gatggcagac ttttcctttg cgcgtcacgg
    14701 tcagcaggcg ggctgtcacc tcatttccca gtcccggcca cttttttcgt gcctgcgggc
    14761 tggttttcag tttcaccaga tgatcgcctt tacccagttt tctgatctct tcatattgcg
    14821 ctccctttct gagagggatt atccagtggc ggtgttctcc cgccaggctc caggcattta
    14881 acagtcctgg tgagtaataa cctttatcca ttaacgtcag aatgttatcg ccggtttgtt
    14941 ctataagttg ctcagcaagc tcattttcgc tgttcttcat cgtgccgaag gctgcagccg
    15001 tcagcagatg gctggtcagt tccatctggc agaccatttt gacctgcggg tagagcaccg
    15061 ggctcccggc atgtgtctgg cgggggaagg ctgcatcgtt ctctggtgta tccggtgtgt
    15121 gccagaacac accatcgatg gccagcaggg tcaggccgca ccagtgcgga tgcggcgtgg
    15181 cgttatgcca gagctgcgct gttttcgtga acacacgcgg cggacagcct cacttcccag
    15241 acgctggcgg gcctgaataa cggcactggg acaacgaagg ggcgattgcc cggcagcatg
    15301 atgtccaggc gattcacaat ctggtgaaga ggttctttac ggtaatgttc cggggacctc
    15361 atcttcacag aaattttcac gccattagca ttccggtatt cgtataactt ccttctgtgg
    15421 agagtaagca atgcccagga tgcgatagac gcttgatcag aaaaaacatc acgttgctgc
    15481 atggcgcgcc agcggcctta cccgtgaaca gtactgtgaa ctttacgaca ttccgtttaa
    15541 atctctgcgc cagtggccgc aggatgttgc aaaagccgaa aaacgggcca gagcaccaga
    15601 aattattcct gtttcagtat ctggtactcc tggaatgcct gatggcagac cattgttgga
    15661 tgagcctgtc acactcttcc tgcccggggg gatccggatg tgctgccagc cttctcagtt
    15721 aaccgatgtt ttccgggctc ttcgccatgc tgacgcctga tcacgtattc ctggtcgtca
    15781 gtcctgtcga tatgcgacgg ggtatcgata cactgacaca gtacgtccag cacgaactta
    15841 acgctgcatg gcatgacggt gctgcttttg tcttcaccaa taaaacccgc tcccgcataa
    15901 aagttctccg gtgggacaaa catggcgtat ggctgtgtac ccgacgactg caccggggaa
    15961 ccttccgctg gccccggaaa ggggatgcca catggcatct cacaccggat gagtttcact
    16021 ggctggtctg tggggttgac tggcagcagg tgaaagggca tgacctggca aaatgggtat
    16081 accaggaaaa aaccgcagat gctcagagca acgcaataac tcaatgaaat ataatgaata
    16141 tccatgcgtg atgtcgtata atccaccgca tggatatctc gcttctcagt accacccgtg
    16201 accctgaaca gcttcgcgct ctggcaattg ccatggtgca aaaagccatg actgagagtc
    16261 agaatcttgc aaatgtggtg ctggaaaaag accggaatat tgcagaactt cagaaccgta
    16321 tccgcatcct ggaagagcag atgaagctcg cacgccagca gcgttttggt aaaaaatgcg
    16381 aatcgctggc cgggatgcag cgttccctgt tcgaagagga tgttgacgcg gatatcgcag
    16441 aaatctcggc gcatctggac agactacttc ctcagaccgg ggacgaagaa aaaacaacaa
    16501 cacgtcctgt ccgtaaacct ttaccctcac atcttccccg ggttgaaaag gttatcccgg
    16561 cagctgagga acgttgtcct gactgtgatg aacccctgca cttcattcgc gatgaagtca
    16621 gcgagaagct ggaatatatt ccggcacagg tcgtggtcaa ccgctatatc cgtccgcagt
    16681 acagctgtcc ctgctgtgag aaagtgttct gcggaaaaat gccggcacat atctttccga
    16741 aaagtgccgt tgagccgtcg gtaattgcac aggttgtcat cagcaaatat accgaccatc
    16801 tgcccctgta tcgccagcag cacattttct cccgaatggg tgtggagctc cccgtcagta
    16861 caatggcgga tatggtcggt gtcgccggag ctgctctggc cccgctggca aaactgttac
    16921 gcctcgagct tctgacacgg gatgttattc atgctgatga aacgtccctg aggctccttg
    16981 atacccggaa aggcggtaaa tcctgctccg gctggctgcg ggcttacgtc agcggtgaaa
    17041 gaagcggtcc gccggtcgtc tgttttgact cgcagaccgg acgggccctc agatatcctg
    17101 aggcctggct gcagggctgg cgtggtggca cgctggtgag tgacggatac aacgtgtaca
    17161 aatctctggc ggataatcac cctggtatca catccgcctg ttgctggagc cacgcaaggc
    17221 gcggcttcgc aaatctgtac aaggccagca gggagccccg tgcagccatg gtgctgagaa
    17281 aaatcgccgg tctttaccgc attgagaagc tcatccgcga gcgtcctgtg gaaaaaatac
    17341 ggcagtggcg ccagcggtac tcccggccga tagtgaatga cctgtttgcc tggcttgaag
    17401 agcaggaacc gtgctgtcag ccagacggac cactaaataa agcgattaac tatatcctga
    17461 accggcgcga tgaactgagc tgcttcctgg gtgacggagc cgtacctctg gacaataata
    17521 tctgcgagcg tgccattcgc tcggtggtca tgggacggaa agcatggtta ttcgcaggtt
    17581 cactaatggc ccgggaaccg cgcggcacag ataatgagcc ttctggaaac ggcaaagcgt
    17641 aatggtctgg agccatattc ctggctgaca gacgtcctga cgcgtctgcc agagtggtcg
    17701 gaagaccgac tggcagagtt gctgcccctt gagggattta ccttctccgg gtgagtgata
    17761 cctgccgtca ggtgttcgct caccgggcca taacctgcag tcgggagttg aacgcctgat
    17821 gacaggaaat gagccagagc agcaaccacg cgggctgaca gcgaatcaga acctgaatca
    17881 tccgggagtc attcccgggt cagtacttca tcacctgcca ccaatacccg agtcagtact
    17941 tcgcgacctg cacaattacc cgactcagtg cttcatagcc cgccgccggt acctgcccgg
    18001 cgctcgcccc tgcgcaagca aaataaatca accatcgtca aactgcgatc gttcgtatgc
    18061 accattcgta acaccgggga ctgatacgcc cgaaaaatgc gtcaatatga gttcatcaga
    18121 acgatacaat tgtcgtggga aagactgatc atttgtcacc taatactcag ctacataaga
    18181 taattaataa attatctata gcgtt
//
LOCUS       DBBGLQ010000086        17831 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.086, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000086 DBBGLQ010000000
VERSION     DBBGLQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.086"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..176
                     /locus_tag="ACIYSB_003913"
     CDS             <1..176
                     /locus_tag="ACIYSB_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430851.1"
                     /translation="DKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVKFFTIAY
                     NLLRNKQLPQPSK"
     gene            complement(586..1719)
                     /gene="thiH"
                     /locus_tag="ACIYSB_003914"
     CDS             complement(586..1719)
                     /gene="thiH"
                     /locus_tag="ACIYSB_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGC5430852.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDSYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(1716..2486)
                     /gene="thiG"
                     /locus_tag="ACIYSB_003915"
     CDS             complement(1716..2486)
                     /gene="thiG"
                     /locus_tag="ACIYSB_003915"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGC5430853.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(2488..2688)
                     /gene="thiS"
                     /locus_tag="ACIYSB_003916"
     CDS             complement(2488..2688)
                     /gene="thiS"
                     /locus_tag="ACIYSB_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGC5430854.1"
                     /translation="MQILFNDQAMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(2672..3427)
                     /gene="thiF"
                     /locus_tag="ACIYSB_003917"
     CDS             complement(2672..3427)
                     /gene="thiF"
                     /locus_tag="ACIYSB_003917"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGC5430855.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(3420..4055)
                     /gene="thiE"
                     /locus_tag="ACIYSB_003918"
     CDS             complement(3420..4055)
                     /gene="thiE"
                     /locus_tag="ACIYSB_003918"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGC5430856.1"
                     /translation="MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(4055..5950)
                     /gene="thiC"
                     /locus_tag="ACIYSB_003919"
     CDS             complement(4055..5950)
                     /gene="thiC"
                     /locus_tag="ACIYSB_003919"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGC5430857.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(6031..6131)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6183..6659)
                     /gene="rsd"
                     /locus_tag="ACIYSB_003920"
     CDS             complement(6183..6659)
                     /gene="rsd"
                     /locus_tag="ACIYSB_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGC5430858.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            6754..7527
                     /gene="nudC"
                     /locus_tag="ACIYSB_003921"
     CDS             6754..7527
                     /gene="nudC"
                     /locus_tag="ACIYSB_003921"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGC5430859.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            7567..8631
                     /gene="hemE"
                     /locus_tag="ACIYSB_003922"
     CDS             7567..8631
                     /gene="hemE"
                     /locus_tag="ACIYSB_003922"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGC5430860.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            8641..9312
                     /gene="nfi"
                     /locus_tag="ACIYSB_003923"
     CDS             8641..9312
                     /gene="nfi"
                     /locus_tag="ACIYSB_003923"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGC5430861.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            9355..9945
                     /gene="yjaG"
                     /locus_tag="ACIYSB_003924"
     CDS             9355..9945
                     /gene="yjaG"
                     /locus_tag="ACIYSB_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGC5430862.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            10132..10404
                     /gene="hupA"
                     /locus_tag="ACIYSB_003925"
     CDS             10132..10404
                     /gene="hupA"
                     /locus_tag="ACIYSB_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGC5430863.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            10417..11112
                     /gene="yjaH"
                     /locus_tag="ACIYSB_003926"
     CDS             10417..11112
                     /gene="yjaH"
                     /locus_tag="ACIYSB_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGC5430864.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(11114..11539)
                     /gene="zraP"
                     /locus_tag="ACIYSB_003927"
     CDS             complement(11114..11539)
                     /gene="zraP"
                     /locus_tag="ACIYSB_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGC5430865.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            11777..13174
                     /gene="zraS"
                     /locus_tag="ACIYSB_003928"
     CDS             11777..13174
                     /gene="zraS"
                     /locus_tag="ACIYSB_003928"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGC5430866.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRASEA
                     DRQALLEKGNVLIRALESGSRVGMGMRMHHVQQQALLEEMAGQPGVLWFAVTDAQGII
                     ILHSDPDKVGRALYSPDEMQKLKPEENSRWRLLGKTETTPALEVYRLFQPMSAPWRHG
                     MHNMPRCNGKAVPQVDAQQAIFIAVDASDLVATQSGEKRNTLIILFALATVLLASVLS
                     FFWYRRYLRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPA
                     GGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANS
                     REIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKIS
                     VTDSGKGIAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTF
                     TLWLPVNITRKDPQG"
     gene            13171..14496
                     /gene="zraR"
                     /locus_tag="ACIYSB_003929"
     CDS             13171..14496
                     /gene="zraR"
                     /locus_tag="ACIYSB_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGC5430867.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(14493..15782)
                     /gene="purD"
                     /locus_tag="ACIYSB_003930"
     CDS             complement(14493..15782)
                     /gene="purD"
                     /locus_tag="ACIYSB_003930"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGC5430868.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(15794..17383)
                     /gene="purH"
                     /locus_tag="ACIYSB_003931"
     CDS             complement(15794..17383)
                     /gene="purH"
                     /locus_tag="ACIYSB_003931"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGC5430869.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKLDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         4013 a         4794 c         4904 g         4120 t
ORIGIN      
        1 gagataaggc attttacttt gttgcgttca gggctttcag ggaaaagaaa aaactccgtt
       61 tgcatgtaac tagcgcttat cccatttctg aaaaacagaa aggtaaatca gtgaaatttt
      121 tcaccattgc ctacaactta ttgagaaata agcagcttcc tcagccctca aaataacaaa
      181 acccacctta aggtgggttt cgccagagaa ttatctctgg tattcagaac gccattaccg
      241 gactttgcct tgaccttgcg ataatcgcag gttgcgggat gtctgaattt cttcagtctg
      301 ctgcatcctg gaagatgaga acatgtgttc ttattttcgt ctctatcata gttgagtatt
      361 tactctctta caatcagatc tctttcattg ctcaacaggc gatggcttca gactttgcat
      421 tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa gcttttcgcc ctggacaaca
      481 ttcctgctga cggcactacc ataaaaaaac acaacgcttt caacaacctt gcgtttttca
      541 tcttccgtag cggctcggaa taaatttacg ttcttacatt ccgtctcata gtctttgcga
      601 ggcgcgtccc agatagctgt cccagtcttt ccataccggc tgcaaaccct gagcggttaa
      661 cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt gagaactgtt ccaactcggg
      721 gtgattatcg gcatagccac ctggctgcgt tttcgagaag gcgctgacgt tattgatcgc
      781 cagcggaata acgcgatcgc gaaaccacgg tgattcccgc gtggagagtg acagttcaat
      841 ctccggtgca agcagtcgga aggcgcagat ggtttgcact aactggcgtt catccataat
      901 cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc ggaaaggaga cagagtaacg
      961 gctttgccag taatgctgtt gcagccatag caaatgttcg gcaaccatat agctgtcaac
     1021 gcgccagttg tcggaaaggc caattagcgc gccgaggcct atcttatcaa tccccgcacg
     1081 ccccagccga tccggcgttt ccagccgcca gaagaagtcc tgttttttgc ctttcagatg
     1141 atggcgggca taagtcgcct cgtgatatgt ctcctgataa accatcacgc catccagacc
     1201 aagttgcttt aactcggcgt attccgtctc cgccagcggt tgcacttcca tctgtagtga
     1261 agagaactgt tcacgaaggg cagggagatg acgacgaaag taatccatcc ccactttcgc
     1321 ctgatgttca ccagtgacta acagcagatg ttcaaagccc atctcccgta tagcggcact
     1381 ttccctggca atatccgctt catccagcgt tttgcgcttg atgcgattac tcatggaaaa
     1441 tccacagtac gtgcagtcgt tagcgcaaag attggaaaga taaagcggga cgtagaaact
     1501 aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc cgttgggcca gttgttccag
     1561 atagccactg gcggcaggcg ataacagcgc catcatgtcg tcgcgggtga gttgcgaggc
     1621 atttagcgcc cgctctacgt cagcagccgt tttgccgttg atacgcaggc ggatgtcgtc
     1681 ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg ccgatgcctc cagaaatccg
     1741 gtcagcgggc tggtggcatg agcaaaataa ctgcggctgc ccggtccgga ctgacgtgcc
     1801 agtaggcctg cttctaccgc cagacgaaat gccttcgcca tgttgacggg atcgtccgcg
     1861 acggcaatcg ccgtattcac taacaccgcg tcggccccca tttccagcgc ctgcgcggca
     1921 tggctgggaa cgccgatgcc agcatcgaca accaccggca ctgtggcctg ctggataata
     1981 atctccagca tggcgcgggt ttccagtccc tgattcgagc caatcggcgc gccgagcggc
     2041 atcaccgctg cacagccgac ttcttccaga cgtttacaca ataccggatc ggccccgcag
     2101 taaggcagca cgacaaatcc ctgttgtacc agcgtttcgg cggctttcag ggtttcgatg
     2161 ggatcgggca acagccagcg ggcgtcaggg tgaatctcta attttaacca gtttgtgcct
     2221 aacgcttcac gagccagatg ggcggcgaaa atggcttctt ccgctgtttt cgccccggat
     2281 gtatttggca gcagggtcac acccgccgcg ataagcggtt cgaggatagc gtcgttgtgc
     2341 tggcgcaagt cgacacgttt catcgccagt gtcaccagct ggctgccgga agcgcggatc
     2401 gcctccacca tcagttgtga agaagcgaat ttccctgtgc cggtaaacag atgtgaatca
     2461 aacgttttgt ccgcaatacg taacatttca accccctgca ataacctgaa aaagcaggat
     2521 ctggtcgcca tcctgcacga tatgttgcgc ccactgctca cgcgggacga tttgctgatt
     2581 aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc tccagtagtt cgtgaacagt
     2641 ttgcccggcg gcgcactgca tcgcttgatc gttaaacagg atctgcattg cttcctccgc
     2701 ataccgggca accactggcg cggcgcaacg ccaggctgcg ccactggctc gatttaccgt
     2761 cgaacagtcg gagttctccc gcaggtgtct ctataccgct taataactta atggcttcca
     2821 gtgcctgcaa agtgcccata accccgacca ccgggccaac cacgcccgcc gtgcggcagt
     2881 tgcgttctgg ctcctggtta tctggccaca ggcagcggta acacccctgc tcccagggcg
     2941 gcgtcagtac catcaactga ccgccaaatc cgaccgcgct ggcggtgata agcggcgtgt
     3001 tgagtgccac gcaggcggca ttaatctcct ggcgagtcgc catattgtcg gtacagtcga
     3061 gcaccacatc ggcccgtgca accgcatctt ttaacgcctc acccgttaac cgttgttgta
     3121 atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg ctgttggctg acctgcgatt
     3181 tcgggcgatc gatatcttca gtggtaaaga ggatttgtcg ttgcagattg cttaaatgca
     3241 catcgtcgtc atctgccagt accagcgtcc cgacgccagc gcccgccagg tacagcgcag
     3301 caggtgtacc cagcccgccc agaccgataa tcagcacctg gctgtcgagc agtttttgct
     3361 gcccgtccag agcgatatcg tcgagcagga tttggcggct ataacgcata aagtcacggt
     3421 cattcatcgc caactcctgc aatttccagc aactgtgccg ttgccaaacg ccagtctgcg
     3481 gcttgagtaa tggcgctgac gacggcgata ctgccgacac ccgttgctat caccgcaggc
     3541 gcgcgtgcca gactgatacc gccaatcgcc acggtgggat aatccgccag tcgctcaaca
     3601 tgccgtgcca gctgttccag cccctgcggt gcagaaggca tctgtttggt ttgcgtcggg
     3661 aacacatgtc ccagcgcgat ataagagggg cgtgctgcca gcgcgacgtc gatttccata
     3721 tcgtcatgtg tcgaaacgcc cagccgcagg cctgccgcgc ggatggcatt gagatcggtg
     3781 gcttgcaaat cttcctgccc caaatggacg ccatacgcct gatgcttgat cgccagccgc
     3841 cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc ccagcgcaat tgccgccacg
     3901 acatcggctt ccacctcttc atcgcgccga tctttgatgc gtagctggag agtacgtacg
     3961 cctgcatcca acagacgttc gatccactgt acgctgtcca ccaccgggta cagtcctgaa
     4021 cgaaaaggta caggaggaaa atcaggctga tacatcacgc ttcctcctta cgcaggtaga
     4081 tttctccgcc tctggcacgg aagttctccg acatatccgc cattcccatt tcaatagttt
     4141 gcgtggcggc gtaatcacgc acttcctggc tgattttcat cgagcagaat ttcggcccac
     4201 acatggagca aaaatgggcg actttacctg actcttgcgg cagggtttca tcgtgataag
     4261 cgcgggcggt aaacgggtcg agggccagat taaactggtc ttcccagcga aattcgaagc
     4321 gggctttcga catggcgtta tcgcgaattt gcgcgcccgg atgccctttc gccaggtcag
     4381 cggcgtgggc ggcaatctta taggtgataa gcccctgctt aacatcttct ttattgggca
     4441 gacccagatg ctcttttggc gttacgtaac agagcatcgc gcagccaaac cagccaatca
     4501 tcgccgcacc aatccccgac gtgaagtggt catagcccgg cgcaatatcg gtagttagcg
     4561 gccccagagt gtaaaacggc gcttcgtggc agtgctctaa ctcctcggtc atattgcggc
     4621 ggatcatctg catcggcacg tggcctgggc cttcaatcat cacctgcacg tcatattccc
     4681 aggcaatttt ggtcagttcg cccagcgtat gcagctcggc aaactgcgct tcatcgttgg
     4741 cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga cagcgaaacg tcataagcgg
     4801 cacagatttc acaaatttcg cggaagtgtt gatagaggaa attttcctga tgatgggaga
     4861 ggcaccattt cgccataatc gaaccgccgc gagagacgat accggtcagg cgtttcgcgg
     4921 tcatcggcac atagcgcagc agtacgcccg catggatagt gaagtaatcc acaccttgct
     4981 cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt aagatcttcg gcgatcccgt
     5041 taaccttctc cagcgcctgg tagatcggca ctgtaccgat cggcaccggg ctgttacgca
     5101 aaatccactc gcgggtttcg tgaatatagc gaccggtgga gagatccatc accgtatccg
     5161 ctccccagcg cgtggaccat accagctttt ccacttcttc ttcgatggaa gaggtgaccg
     5221 ccgagttgcc gatattggcg ttaactttta ccaggaaatt gcgaccaata atcatcggct
     5281 ccgattccgg atgattaatg ttggccggga taatcgcacg tccggcagca acttcatcac
     5341 ggacaaattc cgcagtgata ttttccggca gatgtgcgcc aaagctcatt cccggatgct
     5401 ggtggcgtaa aacctcgcta cggatgcgct cgcggcccat attctcgcgg atggcgatga
     5461 attccatttc cggcgtgatg atgccctggc gggcgtagtg cagttgggtg acacggcgtc
     5521 ctgctttggc gcgttttggt gttagtacgc cgctaaaacg cagttcgtcg aggccatcat
     5581 ctgccagccg cgctttagtg taatcggaac tgcgcacggt aagttcttcg gtatcgccgc
     5641 gcgcatcgat ccacggctgg cgtagttttg ccagcccttg ctgcacgtta atggcaatct
     5701 gcggatcacc atacgggccg gaggtgtcgt agaccggaat cgcttcgttt tcttcgtact
     5761 gcggctgttc tttgctaccg ccaattagcg tcgggctaag ctggatctca cgcatcggca
     5821 cgcgcacgcc ggggtgtgtg ccagtgatat aaatgcgttt tgagttggga aaggcggtgc
     5881 cttccagggt gtcgataaaa tgttgggccc gggcgcgttg ttcgcggcgg gtcagttttg
     5941 ttgcagacat agctcattcc aaaaagttaa ggacgtggct tgtcagacga cggatgaagc
     6001 aagagacgat cgccgcaggg gcgatgcgat agcagattaa ctcttgttcc cttcgcaggt
     6061 attagcctga tcaggttccg cggatcccga ataaacggtc tcagccagtt aaggcactcc
     6121 gacaagaaat tagccccgca aatggggcat tgaatgtaaa ttacgcgtta acagcgcaga
     6181 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
     6241 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
     6301 taattatcat gatcgatggc ggtttccaga ctggaatcgt agtaatccat aatctgttga
     6361 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
     6421 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
     6481 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
     6541 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
     6601 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
     6661 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
     6721 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
     6781 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
     6841 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
     6901 cagggggaac ctgtttggtt agtacaacag cagcggcgtc acgatatggg gtcggtacgt
     6961 caggtcattg atctcgatgt tgggctgttt caactggccg gacgaggcgt acaactggcg
     7021 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
     7081 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
     7141 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
     7201 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
     7261 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
     7321 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
     7381 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
     7441 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatacggtg
     7501 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
     7561 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
     7621 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
     7681 acgcgcgccc aggcaggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
     7741 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
     7801 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
     7861 tttacctcgc cagtcacctg caaagctgac gtcgataaac tgccaattcc ggacccggaa
     7921 gatgaactgg gttacgtgat gaacgcggtg cgtaccattc gtcgcgaact gaaaggcgaa
     7981 gtgccgctga ttggtttttc cggcagcccg tggacgctgg cgacctacat ggtggaaggc
     8041 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
     8101 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
     8161 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
     8221 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
     8281 gacggtcgcc gcgtaccggt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
     8341 atggcagaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
     8401 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
     8461 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
     8521 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
     8581 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta aggagtgatt
     8641 atggatctcg cgtcattacg cgctcaacaa atcgaactgg cttcttctgt gatccgcgag
     8701 gatcgactcg ataaagatcc accggatctg atcgccggag ccgatgtcgg gtttgagcag
     8761 ggcggagaag tgacgcgagc ggcgatggtg ctgctgaaat atccctcgct tgagctggtc
     8821 gagtataaag ttgcccgcat cgccaccacc atgccttaca ttccaggttt tctttccttc
     8881 cgcgaatatc ctgcgctgct ggcagcgtgg gagatgctgt cgcaaaagcc ggatttagtg
     8941 tttgtcgatg gtcatgggat ctcgcatcct cgccgtcttg gcgtcgccag ccattttggc
     9001 ttattggtgg atgtgccgac cattggcgtg gcgaaaaaac ggctctgcgg taaattcgaa
     9061 ccgctctcca gcgaaccggg cgcgctggcc ccactgatgg ataaaggcga gcagctggcc
     9121 tgggtctggc gcagcaaagc gcgctgtaac ccgttgttta tcgctaccgg ccatcgggtc
     9181 agcgtggaca gcgcgctggc gtgggtacaa cgctgcatga aaggctatcg tctgccggag
     9241 ccaacgcgct gggcggacgc ggtggcctcg gaacgtccgg cgttcgtgcg ctatacagca
     9301 aatcagccct aattcaggta aactgcggat aatttccgta tttgagaact caacatgtta
     9361 caaaacccaa ttcatctgcg tctggagcgc ctggaaagct ggcagcatgt cactttcatg
     9421 gcttgcttat gcgaacgcat gtaccccaat tacgccatgt tctgccagca aaccggtttt
     9481 ggtgatgggc aaatttaccg tcgcattctc gatctcatct gggaaacgct gaccgttaaa
     9541 gacgcaaaag taaatttcga cagccaactg gagaaatttg aagaagcgat tccttcagcc
     9601 gacgatttcg atctgtacgg cgtttatccg gcaatcgatg cctgcgtggc gttaagtgaa
     9661 ctggtccatt cgcgtttgag tggtgaaacg ctcgaacacg cggtggaagt gagtaagacc
     9721 tccatcacga ccgttgcgat gctggaaatg actcaggctg gtcgcgaaat gagcgatgaa
     9781 gagcttaaag aaaacccagc tgtagagcaa gaatgggaca ttcagtggga aatattccga
     9841 cttttagctg agtgcgaaga acgcgatatc gagctgataa aaggccttag ggcagacctg
     9901 cgtgaggcgg gtgagagcaa tattggtata atttttcagc aataagacca gaaaacgtga
     9961 tttaacgcct gatttgtcgt acctggagtc ttccctttcg ccccccgtct ggtctacatt
    10021 tggggggcga aaaaaagtgg ctatcggtgc gtgtatgcag gagagtgcta ttctggcatt
    10081 tccgtcgcac tcgatgctta gcaagcgata aacacattgt aaggataact tatgaacaag
    10141 actcaactga ttgatgtaat tgcagagaaa gcagaactgt ccaaaaccca ggctaaagct
    10201 gctctggagt ccactctggc tgcaattact gagtctctga aagaaggcga tgctgtacaa
    10261 ctggttggtt tcggtacctt caaagtgaac caccgcgctg agcgtactgg ccgcaacccg
    10321 cagaccggta aagaaatcaa aattgccgca gctaacgtac cggcatttgt ttctggcaag
    10381 gcactgaaag acgcagttaa gtaagattgc gtggcagtga acagttttaa cgaaggggtg
    10441 gtttcacccc ttttgtcttt ctggcgtcga tcattgatgc tggctggcgc tctgcttctc
    10501 actgcctgta gtcataactc ttcacttcct ccctttaccg ccagtggatt tgctgaagac
    10561 cagggcgcgg tacgcatctg gcgaaaagac agcggcgata atgtgcatct gcttgccgtg
    10621 tttagcccgt ggcgcagtgg cgataccacg acgcgagagt atcgctggca gggcgataac
    10681 ctcacgctca tcaatatcaa tgtttacagc aaaccgccgg tgaatattcg cgcgcgtttt
    10741 gacgatcgcg gtgatctgag ctttatgcaa cgtgaatccg atggggaaaa gcagcagctt
    10801 tctaacgacc aaatcgattt ataccgttat cgtgctgatc agatccgcca gattagcgat
    10861 gccttacgtc aggggagagt cgtgctgcgc caggggcgct ggcatgcgat ggaacagacc
    10921 gtgaccacct gcgaagggca aaccattaaa cctgatttag attcgcaggc gatagcgcat
    10981 atcgagcgcc gccagagccg ctcttctgtt gatgtcagcg tggcatggct ggaagcgccc
    11041 gaaggttcgc aattactgtt agtggcaaac tctgatttct gtcgctggca acccaacgag
    11101 aaaacgttct gatttaccag tggcccatac ccatatgacc gccaccaccg cagccgccgt
    11161 agcccatgcc cattccggct ccgcgcggaa tacccgcttc agccatcgcg atatctcgtt
    11221 tcacccgtaa ctcatctaac gactgacgca aattctccat ctctttggcg accgcgttaa
    11281 ttttgctgct atccggtggg ttcgcggcta acagggcatt gtattcataa cgcttcgtca
    11341 ccagttgctg ttgcagtgcg ctgctttgag cgtaaaagtc attatggatt ttctgccacg
    11401 ctgtctgttg ttcgctggtc aaaggcgcgg cattttgctg ccacataccg tgtccgccgt
    11461 gagcaaatgc agatgtcgat cccatcgcca ttgctgaaag cgccatcatt accagggcaa
    11521 ttttcgtgtt ccgtttcatg gttaatcctc cagtggttgt cttacttcgg gtattgcatc
    11581 ttccgtgcca acgatgaaac gctgatatga cgggtaatct ggcatgataa acgagtaaaa
    11641 atgactcgcc tgctgcgggt agcgagtcat ttttactcat tgaaacttaa gcctttgtgt
    11701 tacagcgcag ggtaagcgct gataaaagat ggcatgattt ctgctgtcag aaagggatga
    11761 gcaggcaaag aagaagatgc gttttatgca acgttctaaa gactccttag ctaaatggtt
    11821 aagcgcgatc ctccccgtgg tcattgttgg gctggtggga ttgtttgcgg taactgtgat
    11881 tcgtgattat gggcgggcaa gcgaggcaga ccgccaggca ttactggaaa aaggtaatgt
    11941 gcttatccgc gctctggagt cgggaagccg cgtagggatg gggatgcgaa tgcaccatgt
    12001 acagcaacag gcgcttctgg aagagatggc gggacagccg ggagtgttgt ggttcgcagt
    12061 caccgatgcg cagggcatca ttattcttca tagcgacccc gataaggtcg ggcgtgcgct
    12121 ctattcgccg gatgaaatgc agaaattaaa gccagaggaa aactcccgct ggcggctgct
    12181 tgggaaaacg gaaactacgc ctgcacttga ggtctatcgt ttgttccagc caatgtcagc
    12241 gccctggcgg catggaatgc acaatatgcc gcgctgtaac ggcaaagctg tgccacaagt
    12301 agatgcacaa caggctattt ttatcgccgt tgatgccagt gatctggttg caacccagag
    12361 tggggaaaag cgcaataccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    12421 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    12481 aatgaagcgc aaagagaagc tggtggcgct ggggcatctt gcggcaggcg ttgcccacga
    12541 aatccgtaac ccactttcct cgattaaagg actggcgaaa tactttgccg agcgcgcgcc
    12601 tgcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaggccg accgtttaaa
    12661 ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt
    12721 ggatctcaac acgctgatta accactcatt acagctggta agtcaggatg caaacagccg
    12781 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgacccgga
    12841 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttggtcagca
    12901 tggcgtgatt agcgtgacgg ccagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    12961 cagcggtaag ggaattgcgg cagatcagct tgatgccatc ttcactccgt acttcaccac
    13021 taaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    13081 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    13141 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    13201 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    13261 aacgtcgcgc tggcgaacag cgggcgacag gcgttggagc aggtgcggga acaggttttt
    13321 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    13381 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    13441 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    13501 gataacctac aggcgacgct ggaaaaagcg ctcgcgcata cgcacagtat tgatgctgaa
    13561 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    13621 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cggtactgat ccacggcgat
    13681 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    13741 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    13801 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    13861 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    13921 gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc gtgtcggtag caaccagatt
    13981 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    14041 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    14101 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    14161 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    14221 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    14281 ctgctgaccg gggaatatat ttccgaacgc gagctgccgc tggcgattgc cagtacgccg
    14341 atcccgctgg gacaaagtca ggatattcag ccgttggtgg aagtggaaaa agaggtgatt
    14401 ctggcggcgc tggagaaaac gggcggcaac aaaaccgaag ccgcccgtca gttagggatc
    14461 acgcgcaaaa cgctattggc aaaactgtcg cgttagttct gctcgcgttc gatagcgcgc
    14521 cagccgatat ctttccggca gaagcagtcg tcccagtgga tatcggtcat taaggcatag
    14581 gcgcgtttct gcgcttctgc cacggtatga cccagcgcgg tgacgcacag tacgcgcccg
    14641 ccgttggtca ctacctgctc gtcatccgcc agttttgtgc ccgcgtggaa cactttgccg
    14701 cctgccactt cttccagcgg caggccgtgg atcacgtcac cggtgcggta atcacccgga
    14761 tatccacccg cagccatcac cacgccgaga gaagcgcgtt catcccactc ggacgttttc
    14821 tcgtccagtt tgctttcaca ggccgccagg cagagctcaa ccagatcgga cttcatgcgc
    14881 agcataatcg gctgggtttc cggatcgcca aagcggcagt taaattcaat aaccttcgga
    14941 ttgccctgtt tgtcgatcat caggcccgcg tagagaaaac cggtgtaggt gttgccttcc
    15001 gccgccatgc ctttcacggt tggccagata atacgttcca tggtgcgctg atgaacgtca
    15061 tcggttacta ccggcgcggg ggagtaagcg cccatcccgc cggtgtttgg tccggtatct
    15121 ttatcgccta cgcgtttgtg atcctggctg gtagccatcg gcagcacatg ctcgccgtcc
    15181 accatcacga taaagctcgc ttcttcgcca tcgaggaact cttcgataac gatgcgatga
    15241 cccgcgtcgc caaaagcgtt gcccgccagc atatcgtgaa cagccgcttc cgcttcttcc
    15301 agcgtcatcg ccacgataac gcctttcccg gcagccagac cgtccgcttt aatgacgatt
    15361 ggcgcgcctt tctcacgcag atacgccagc gcaggttcta cctcggtgaa gttctggtat
    15421 tccgccgtag ggatcttatg gcgggccagg aaatctttgg taaacgcttt tgagccttcc
    15481 agttgggccg cacctgcggt tgggccgaag attttcagcc cggcggcgcg gaaggtatcg
    15541 accacgcctt tcaccagcgg cgcttccggg ccgacgatgg tcagatcaat cttttcgttt
    15601 tgtgcgaaat ccaacagcgc cgggatatcg gtcacgccaa tagcaacgtt ttgcagcgcg
    15661 ggttccagtg cagtgcctgc attacccgga gcaacaaaaa cagtctcaac cagcggcgac
    15721 tgggccgctt tccaggccag cgcgtgctcg cgcccgccgt taccaatcac taatactttc
    15781 atctattgct ccattaatgg cggaagtggc gcatgtcggt gaagagcatc gcaataccgt
    15841 gctcgtcggc ggcggcaatc acttcgtcat cacggataga accgccaggc tggattacgc
    15901 aggtcacgcc cgcagcggcg gcggcatcaa taccgtcgcg gaacgggaag aacgcgtcag
    15961 aagccatcga ggaacctttc acttccaggc cttcatcggc cgctttaata ccggcgattt
    16021 ttgcggagta cacgcggctc atctggcccg cgccaatgcc gatagtcata ttgtttttgg
    16081 catagacgat agcgttggat ttcacaaact tcgccacctt ccagcagaac agcgcatcac
    16141 gcagttcctg ttcgctcggc tgacgtttgg tcaccacgcg cagttcttcc gcaccgacca
    16201 tccccaggtc acgatcctga accagcagac cgccgttcac gcgtttgaaa tcgaggcccg
    16261 gaacacgctc gccccactga ccgcaggtca gaacgcgtac gttctgtttg gcggcggtga
    16321 ttttcagggc ttcttcgctg gcggacggcg caataatcac ttcgacaaac tgacgagaaa
    16381 tgatggcctg tgcggtttcc gcatccagct cgcggttaaa ggcaatgatg ccgccgaatg
    16441 cggaggttgg gtcggttttg tacgcgcgat cgtaagcatc aagaatggaa ttgccgatag
    16501 ccacgccgca agggttggcg tgcttcacaa tcacacatgc cggctcggcg aactctttca
    16561 cgcactccag cgccgcatcg gtatcggcga tgttgttata agagagggct ttaccctgaa
    16621 cctgggttgc ggtagcaacg gaggcttctt tcacattctc ttctatatag aaggcagcct
    16681 gctggtggct gttctcgccg taacgcatat ccagcttctt aatgaagttc aggttcagcg
    16741 tgcgtgggaa gcgaccggcg gcttctttgc tttcaccgtg gtaagccgga accatgctgc
    16801 cgaagtagtt ggcaatcatg ctgtcgtagg cggcagtgtg ttcgaaggct ttgatggcga
    16861 ggtcgaaacg ggttgcaagc gtcagcgatc cttcgttgtc atccatctct ttaataatgg
    16921 cgtcatagtc gctgctcttc accacgattg cgacatcttt atggttcttg gcggcggagc
    16981 gcaccatcgt tgggccgccg atatcgatgt tctcaaccgc atcttccagc gagcaacctt
    17041 cacgggccac ggtctgggcg aacggataca ggttaacaac caccatatcg ataggctgga
    17101 tctgatgttc ttccataatg gcatcgtcct ggccgcgacg gcccagaatg ccaccatgta
    17161 ctttcggatg cagggtcttc acgcgtccat ccatcatctc cgggaaaccg gtgtaatcgg
    17221 aaacttcggt taccggcaga cctttttctg ctaacagacg ggcagtgccc cctgtagaca
    17281 gcagctccac accgcgtgcg gaaagtgcct gggcgaattc gacgataccg gctttgtcag
    17341 aaacactgag cagagcgcgg cggactggac gacgttgttg catggtaaat cccctggatt
    17401 tgactattac agagagcgtt agctgaattt ttcgcgaaaa actcagctaa cgcccctaac
    17461 ggggcatcct tatttttcgc ccgcattgta acgaaaacgt ttgcgcaacg ctcgcgaatt
    17521 tttctctttc aatggtgatc acaattttga ctgtggttac cgtgggcaaa atacagaaat
    17581 tacattgatg attgtggata actctgtgcg taaaaaggta taaagcgggc ttttgctggg
    17641 gaatgcagca gtcagtcatt tttctgcaat ttttctattg cggcctgcgg agaactccct
    17701 ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag ccggttcggt
    17761 tgaagagaaa aatcctgaaa ttcagggttg actctgaaag aggaaagcgt aatatacgcc
    17821 acctcgcgac a
//
LOCUS       DBBGLQ010000087        17734 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.087, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000087 DBBGLQ010000000
VERSION     DBBGLQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.087"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(148..1029)
                     /gene="prmA"
                     /locus_tag="ACIYSB_003932"
     CDS             complement(148..1029)
                     /gene="prmA"
                     /locus_tag="ACIYSB_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGC5430870.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1041..2492)
                     /gene="panF"
                     /locus_tag="ACIYSB_003933"
     CDS             complement(1041..2492)
                     /gene="panF"
                     /locus_tag="ACIYSB_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGC5430871.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2482..2724)
                     /locus_tag="ACIYSB_003934"
     CDS             complement(2482..2724)
                     /locus_tag="ACIYSB_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGC5430872.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLSGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2833..4182)
                     /gene="accC"
                     /locus_tag="ACIYSB_003935"
     CDS             complement(2833..4182)
                     /gene="accC"
                     /locus_tag="ACIYSB_003935"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGC5430873.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4193..4663)
                     /gene="accB"
                     /locus_tag="ACIYSB_003936"
     CDS             complement(4193..4663)
                     /gene="accB"
                     /locus_tag="ACIYSB_003936"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGC5430874.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5641..6615)
                     /locus_tag="ACIYSB_003937"
     CDS             complement(5641..6615)
                     /locus_tag="ACIYSB_003937"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGC5430875.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRVLPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6767..8707
                     /gene="csrD"
                     /locus_tag="ACIYSB_003938"
     CDS             6767..8707
                     /gene="csrD"
                     /locus_tag="ACIYSB_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGC5430876.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSVLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9012..10055
                     /gene="mreB"
                     /locus_tag="ACIYSB_003939"
     CDS             9012..10055
                     /gene="mreB"
                     /locus_tag="ACIYSB_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGC5430877.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10121..11224
                     /gene="mreC"
                     /locus_tag="ACIYSB_003940"
     CDS             10121..11224
                     /gene="mreC"
                     /locus_tag="ACIYSB_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGC5430878.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11224..11712
                     /gene="mreD"
                     /locus_tag="ACIYSB_003941"
     CDS             11224..11712
                     /gene="mreD"
                     /locus_tag="ACIYSB_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGC5430879.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11721..12314
                     /gene="yhdE"
                     /locus_tag="ACIYSB_003942"
     CDS             11721..12314
                     /gene="yhdE"
                     /locus_tag="ACIYSB_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGC5430880.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASDEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12304..13773
                     /gene="rng"
                     /locus_tag="ACIYSB_003943"
     CDS             12304..13773
                     /gene="rng"
                     /locus_tag="ACIYSB_003943"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGC5430881.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13841..17641
                     /gene="yhdP"
                     /locus_tag="ACIYSB_003944"
     CDS             13841..17641
                     /gene="yhdP"
                     /locus_tag="ACIYSB_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGC5430882.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNNKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINSDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNIELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGKITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
BASE COUNT         4237 a         4515 c         4807 g         4175 t
ORIGIN      
        1 cactcaaaga aatttgagta agttctcaat ttttctttat gaatgaaaat tttggcacgc
       61 aaatttgcgt aaataatcgg caaatttacg tgccttttgt gagcttgctc gcacttcgcc
      121 ccgcgtcacc ctacggcgat gcgaaggtta attcttacga ccggtaatac ggcaccactc
      181 ttctttttcc acgaccgggt ccagtgcgaa gctatcggca taagcttcac aaacgctctc
      241 tgcctggctt gccagaatac cggaaaggcc cagcaaaccg cctgaaaccg gcaggacgct
      301 gattaacggt gccagttcac gtaatgggcc tgcaaggatg ttagcgacca ccacgtcggc
      361 tttcatttct tctggctgat ctttcggtaa gtagagttcc agacggtcag aaacgccatt
      421 acgttcggcg ttatcgcggc tggcctgaat cgcctgcgga tcgatatcaa taccaatggc
      481 ttttgctgca cccagtttca gcgccgcgat cgccagaatg ccggaaccac agccaaagtc
      541 gatgactgtt ttaccggtta aatcgaggct gtcgagccat tgcaggcaca gagaggtggt
      601 tggatgggta cccgtaccaa acgccagccc tggatctaac atcacgttga cggcgttttc
      661 gtccggcaca tcacgccagc tagggcagat ccacagtcgt tcaccaaagc gcatcgggtg
      721 gaaattatcc atccattcgc gctcccagtc tttatcttct agttgttcga ttttatgcgc
      781 gaagcctgcg ccgagcagcg gatggttttc cagaatcgcc accacgtcgt tcatatcggt
      841 ttcagcgtcg aacagaccaa tcacatcggt gtcgccccac aggcgcgttt cgcccggcag
      901 cggttcaaat actggcgtat cgtgggtatc ctgaaaagtg atagaaacgg cacccgcttc
      961 catcagcgca tcgctaagat cttccgcgtt cgcgccggtg gtgttcagtt tcagttggat
     1021 ccaaggcatg gcaaaactct ttatttatca gtagtcaaaa cggtagcttg cgggacggat
     1081 gtaccgaaac ggtttccgac caggaaagcc agcaaactta gtagtaacga gggcacgata
     1141 gggtggaagc ccaggtactg aatattcagc gtcgcgagta cggcatacag cacgccgcca
     1201 acgatcatcg cacttagcgc gcctttggcg ttggcgcgtt cccagtaaag acccagcacc
     1261 agcggccaca ggaaaacggc ttccagccca ccgaaggcca acaaattcag ccagatgatc
     1321 atttctggcg gcttccaggc ggcaagcagc agcaacgcgc cgagaactaa cgtaattacc
     1381 gccgacatcc gcttcagacg cgtctcgttt tgcatttgat ccggacggat attcagatag
     1441 agatctttaa tgatcgtagc ggaactttgc agcagttggg cgttaattgt cgacatgatc
     1501 gcagccatcg gtgcagccag gaagatcccg gcagcaaacg gtggcagcac ttttaccatt
     1561 aacgttggga tcaccaggtc cggtacggtg agatcgggga tcaccgcccg acctaacgct
     1621 ccggccaggt gcataccgaa catcagaatt gcgaccacaa tcgtaccgat gatgatcccc
     1681 cgatgtacgg ctttgctgtc tttataagag atacagcgca ccgcagtatg cggcaggcca
     1741 atcacgccaa aacacaccag tacccagaac gacgtcataa aggcaggcga cagaatatcg
     1801 tcagcgcctt gtggcgtaac cagttgcgga tcgatggttt gcaaggtctg tactgcgtta
     1861 cttaagccgc cagcggcatg tactacgcca ataagcagca caacggtgcc aatcagcatc
     1921 acaagccctt gcatggtgtc gttcagcacg ctggcgcgaa agccaccaaa ggcggtatat
     1981 aacgcaatgc tgataccaaa aatcagcagc ccggtttcat aaggaatacc cgccgcggtt
     2041 tccagcaggc gcgcaccgcc gataaactgc acggtcatcg caccaacgaa cgcaaccagc
     2101 aaactcaaac tcgccagcca caccagaaga cgactctggt agcgggcaaa cagcatatcg
     2161 ttcagcgtca ctgcattgta gcggcgcgca agaatcgcaa acttcttgcc gagaataccg
     2221 agtgaaagcc agactgcagg aagctgaatc atcgccagca atacccagcc cagcccgtat
     2281 ttataagcag ctcctggccc gccgataaac gaactggcac tgatataggt cgcggtgagc
     2341 gtcatcgcca gcacaatacc gcccatagag cggctgccga ggaaatactc attaaggaag
     2401 gtgccggtgc tccgtttacg catcgcataa accgagatac cgaacaccac caccagatag
     2461 gcgaccagcg gtagaattac ttcaagctgc atcgtcatcc tccagtggga tatcgcgata
     2521 gataaatttc accatcgccc agcacagtcc aataaacagc agcggcgtca ggatgcaggc
     2581 catctcaaac cagcgcggaa agccggtaaa accgggggca acgccagata agtaagcggc
     2641 tactaaccaa actgccagat acaaaagggt cagccccagc gcccagcgcg cctctttatg
     2701 ggcctgaaca aaacgagtgt ccattttttg tccctgatgg gtgaagaaag cggggattgt
     2761 accttatggg ggttgtcgat ccccagtaat aaaaaaggcc ggaaaatccg gccttttgac
     2821 gctttagcag tcttattttt cctgaagacc gagttttttc tccagatagt ggatgttagt
     2881 gccaccatgc tggaagttct cgtcattcat gatgcggatc tgcagatcaa cgttggtttt
     2941 gataccgtcg atgatcagct cctgcagcgc attcttcatg cgggcaatcg ccacgtcacg
     3001 gttttcaccg tagcaaatca gcttaccgat cattgagtca tagtacggcg gtacggtgta
     3061 gcccgcgtag atatgagact cccaacgtac gccaaaaccg ccaggtgcgt ggaaacgggt
     3121 gattttgccc ggacttggca ggaaggtgtt cggatcttcg gcgttgatac gacattccac
     3181 cgcatggccg cgaacgtgaa cttcttcttg cttgatcgac agcggttgac cggcagcgat
     3241 acgcagctgt tctttgatca ggtcaacgcc ggtgatcatt tctgtaaccg ggtgttctac
     3301 ctgaatacgg gtgttcattt cgatgaaata gaactcgccg ttttcgaaca ggaactcgaa
     3361 agtacctgca ccgcgatagc cgatatcaac acacgcttta gcgcaacgtt cgccgatgta
     3421 gcgacgcagt tccggggtaa tgcccggtgc tggcgcttct tcgaccactt tctggtggcg
     3481 gcgttgcatg gagcagtcac gttccgccag atagatagcg ttgccctgac cgtcagccag
     3541 tacctgaatc tcgacgtggc gaggattttc caggtatttc tccatgtaaa ccatatcgtt
     3601 gctgaaagca gctttcgctt ccgcacgggt catggagatg gattgtgcca gttcagcgtc
     3661 gccgcgcact acgcgcatac cgcgaccgcc gccgccgccg gaggctttga taatcaccgg
     3721 ataaccaatg cgtttagcaa tggcacggtt tttatccata tcgtcgccca gcgggccgtc
     3781 agaacccggt acgcaaggga cgcccgcttt tttcatcgcc gcgattgcgg atactttgtc
     3841 gcccatcagg cgaatggttt ctgctttcgg gccaatgaag ataaagccgg agcgttcaac
     3901 ctgctcggca aagttggcgt tctcggagag gaagccgtaa cccggatgga ttgctactgc
     3961 gccggtgatt tcagcggcgc tgatgattgc cgggatgttc agataacttt ttactgacgg
     4021 agcagggcca atacagaccg tttcatctgc cagtaatacg tgttttagat cgcgatccgc
     4081 gctggagtgc acagcgacag tcttgatgcc cagttcttta caggcacgaa gaatacgcaa
     4141 tgcaatctcg ccgcggttgg caataacaat tttatccagc atgttcgcct cgttactcga
     4201 tgacgaccag cggctcgtca aattctaccg gttgtccact ttcgaccaga attgctttca
     4261 cggtaccgga tttgtccgct tcgatctggt tcatcatttt catggcttca acgatgcaca
     4321 gggtatcgcc cacgttgact ttctgaccca cttcgatgaa cgcttttgcg tccgggcttg
     4381 gggtgcggta gaaagtacca accatcgggg aacgtacgat gtgaccactg atttccgctg
     4441 ctgctggcgc ttccatggaa ggaacggtcg ccggagcggc tgcgttagat tgagctggct
     4501 gctgcatcat tggtgcagcg taagcttgtt gcatcacagg gaaacttgcg gcaggagctg
     4561 cacggctaat gcgtactgac tcttcgcctt cagaaatttc cagttcggag atgcctgatt
     4621 cttcaaccag ctcgatcagt tttttaatct tacgaatatc catgagtggg ttccgtactc
     4681 tttgtttagt gtgattgtga caggcgtttc accgccgtct gtaaagcgta tgccccgaca
     4741 gctgtatgca tagcgataaa ttccagcagg ccgggacgcc ggtctatttt gccccagagt
     4801 gtcaatgtta gacttgacgg acattgtgca gcgatctgct gctatcctcc ggcaaaaaac
     4861 aaaatatacc ttcgttgcgc caatgtcacc ttttccgcag cgcaaaaact gcgtcaggga
     4921 ggacgaggtc gcacattata acgatttcgt agcaattggc agctaaatac tggtcttatc
     4981 agggaagata atcaacagct aacatgtaaa taaccttcaa caccgtgtaa tttgcaacaa
     5041 gccgcacaat tcacgaaatt agcgccatca tcgacggaac ttcttataac gtaaggtgaa
     5101 aagctgaaaa gccagccctg cgaagatgag gggctgcaat gagataatct tcacagacca
     5161 caaataatgt agggatgcca ggatcgcgac aagatagacg acattacgca gcggttgctg
     5221 gggattaccc aattttcact gcatcgtctg gatagatgtg aatgctaaag cacgcaaaat
     5281 taaccgattg acaatgctca gcattaaata ggttcgagtg attaatttgt agtcaaacaa
     5341 aatcagatta ttcaccccat ggtaccagaa ataagtaagg ggctaagcgt agcgtcgccc
     5401 aggcaaagca ccataaacct aacaggcgac ggaggcgtat caataatagc tgtttagtgg
     5461 agtatcaatg cgcatcaatt tgtggtcagt cgaaatcatg actttccagt ttggatgatg
     5521 ttgtataact tcctgaccaa ggtgttcgac gaaagctgtc cggctaaccg cttttcccgt
     5581 taaccgattg aataatgatt tcaaaatgag ttccctctct tattattccc tgctaaatgg
     5641 ttagttaacc ttcaccagcg tgcgaccctg gatctggtta ttaatgatgg cctcggcaaa
     5701 gttcggtgcc tctgacagag atatctcttt tgccgcctgg gtatagaatg attccggtaa
     5761 atcggcgacc agtcgctgcc aggcttgtgc gcggcgttct ggtggcgtca ttactgaatc
     5821 caccccttgc aaacggacat tacgcagaat aaatggcatg accgtggttg gcagagtaaa
     5881 accacccgcc agaccacagg ccgccacgca gccgccgtaa ttcatttgcg ccagcacttt
     5941 tgccagcact ttgtcgccaa cggtgtcaat tgccccagcc cagacttgtt tttccagagg
     6001 acgggattcg gcaaactcat cacgagggag aacacggcta gcacctaaac ttttcagata
     6061 ttcatgggta ctttcgcgac cggaaacggc aacgacctga taacccaact tatgcagcag
     6121 cgccacggcg gtactgccga cgccaccact ggcacccgtc acgacaatct ccccgtcctg
     6181 cgggcgaaca ccggcatctt ccagcgccat cacacacagc atggcggtaa aaccggcagt
     6241 accgataatc attgctttac gcgcgtccag cccttgcggc atggcaacca gccagtcacc
     6301 tttcactcgc gcctgctccg ccagcccacc ccagtggttt tcaccaacgc cccagccagt
     6361 gagtaacacc tcctgaccgg catgaaaacg cggatcttcg ctggtgcgta cagttccggc
     6421 aaaatcgatc ccaggaatca tcggaaaatt acggatgatt tttcccttac cggtaatcgc
     6481 cagcgcatct ttatagttca ggctcgacca gtgaacatcg accgtgacat cgccctccgg
     6541 caggcgactt tcgtccagag tctgtactga tgcgagagtt ttgccgtcct gctgttctaa
     6601 aagtaacgcc tgcataagtg gtcctcatgt gcatgatgga ttggaaaata atttctgaag
     6661 actatactcg ctaatggaaa agcaaagccg atgaagcgca ataaattgcg agataaatct
     6721 gatttgctag tatgcccgct tcctcactat cggagttaac acaaggatga gattaacgac
     6781 gaaattttcg gcctttgtta cgctgctcac cgggttaaca atttttgtga ctttgctggg
     6841 ctgttcgcta agtttctaca acgccattca gtataagttt agtcatcgcg ttcaggcggt
     6901 ggcgacggcg attgataccc accttgtgtc gaatgacttc agcgtattaa ggccacaaat
     6961 taccgaatta atgatgtcgg cagatatcgt tcgtgtagac ctgctccatg gtgataaaca
     7021 ggtttatacc ctggccagaa atggtagtta tcgtccagtt ggctccagcg atctgtttcg
     7081 cgaactgagc gttccgttga taaagcatcc ggggatgtcg ttgcgtctgg tttatcagga
     7141 tccgatgggc aactatttcc attcgttgat gaccaccgcg ccgctcacgg gggcgattgg
     7201 ctttatcatt gttatgctct tcctggcggt acgctggtta caacggcaac ttgccgggca
     7261 agaattgctg gaaacccggg ctactcgtat cttaaacggt gagcgtggct ctaatgtgtt
     7321 gggaaccatc tatgaatggc cgcccagaac cagcagtgcg ctggatacgc tgcttcgtga
     7381 aattcagaac gcacgcgaac aacacagccg tcttgatacg ctgatccgct cttatgccgc
     7441 ccaggacgtg aaaaccggcc tcaataaccg actctttttc gataatcagt tagcaacgtt
     7501 actggaagat caggagaaag taggtaccca cgggatcgtg atgatgattc gtctgccgga
     7561 tttcaatatg ttgagcgata cctgggggca cagccaggtt gaagaacagt tcttcactct
     7621 gacgaatctg ctgtcgacat ttatgatgcg ctaccctggc gcactgctgg cgcgttacca
     7681 ccgcagtgat tttgctgcgc tgttaccgca ccggacgtta aaagaggcag agagcatcgc
     7741 cggtcagtta atcaaagccg ttgatacctt gccgaacaat aaaatgctcg atcgcgacga
     7801 tatgatccac attggtatct gcgcctggcg tagtggtcag gataccgagc aggtaatgga
     7861 acatgcagag tctgccacgc gtaatgcggg attgcagggc ggcaatagct gggctattta
     7921 cgatgactcg ttgcctgaaa aaggacgcgg taatgttcgc tggcgtacgc ttatcgagca
     7981 aatgctcagt cgcggcggcc cgcgccttta tcaaaaaccg gcggttactc gcgaaggtca
     8041 ggttcatcat cgcgaactca tgtgccgcat cttcgatggt aatgaagagg ttagctcggc
     8101 ggagtatatg ccgatggtct tgcagtttgg cttatcggaa gagtatgacc gtctgcaaat
     8161 cagccgtctt attccactat tgcgttactg gccagaggaa aatctggcga ttcaggttac
     8221 cgttgagtcg ctgattcgcc cgcgttttca gcgttggctg cgcgatacgt taatgcaatg
     8281 tgaaaaatca caacgaaaac gcataattat tgaacttgca gaggccgatg taggtcaaca
     8341 tatcagtcgt ttacaacctg ttattcgttt agtgaatgct ttaggggtac gggtagccgt
     8401 caaccaggct ggtttgacgc tggtaagtac cagttggatc aaagaactta atgttgagtt
     8461 actcaagctc catccggggc tggtcagaaa cattgagaag cgaacggaga accagctgct
     8521 ggttcaaagc ctggtggaag cctgctccgg gaccagcacc caggtttacg ccaccggcgt
     8581 gcgttcgcga agcgagtggc agaccctgat tcagcgcggt gttacaggcg ggcaagggga
     8641 ttttttcgcg tcctcacagc cacttgatac taacgtgaaa aaatattcac aaagatactc
     8701 ggtttaacct gccgtttaat ccgttttcac gtagaataac gcgcgctgcg tctcatggga
     8761 gtgtgcttgt ctgctcgcca gattgttgca gcacatatgc agatgaatga ccttacgcgg
     8821 ttgcaaacag gcgaggaatg ctgctgatgc attaagcctt tctggactca ggcagagatt
     8881 tgtaacaaag gaaacgaact gcactaattt tcaccgtagc agatgatttt tgcgccttgt
     8941 cgctgctgcg tgtggttggt aaagtaagcg gattttcttt tccgccccag ctttcaggat
     9001 tatcccttag tatgttgaaa aaatttcgtg gcatgttttc caatgacttg tccattgacc
     9061 tgggtactgc gaataccctc atttatgtaa aaggacaagg catcgtattg aatgagcctt
     9121 ccgtggtggc cattcgtcag gatcgtgccg gttcaccgaa aagcgtagct gcagtaggtc
     9181 atgacgcgaa gcagatgctg ggccgtacgc cgggcaatat tgctgccatt cgcccaatga
     9241 aagacggcgt tatcgccgac ttcttcgtga ctgaaaaaat gctccagcac ttcatcaaac
     9301 aagtgcacag caacagcttt atgcgtccaa gcccgcgcgt tctggtttgt gtgccggttg
     9361 gcgcgaccca ggttgaacgc cgcgcaattc gtgaatccgc gcagggcgct ggtgcccgtg
     9421 aagtcttcct gattgaagaa ccgatggctg ccgcaattgg tgctggcctg ccggtttctg
     9481 aagcgaccgg ttctatggtg gttgatatcg gtggtggtac cactgaagtt gctgttatct
     9541 ccttgaacgg tgtggtttac tcctcttctg tgcgcattgg tggtgaccgt ttcgacgaag
     9601 ctatcatcaa ctatgtgcgt cgtaattacg gttctctgat cggtgaagcc accgcagaac
     9661 gtatcaagca cgaaatcggt tcggcttatc cgggcgatga agtccgtgaa atcgaagttc
     9721 gtggccgtaa cctggcagaa ggtgtcccac gcggttttac cctgaactcc aatgaaatcc
     9781 tcgaagcact gcaggaaccg ctgaccggta ttgtgagcgc ggtaatggtt gcactggaac
     9841 agtgcccgcc ggaactggct tccgacatct ccgagcgcgg catggtgctc accggtggtg
     9901 gcgcactgct gcgtaacctt gaccgtttgt taatggaaga aaccggcatt ccagtcgttg
     9961 ttgctgaaga cccgctgacc tgtgtggcgc gcggtggcgg caaagcgctg gaaatgatcg
    10021 acatgcacgg cggcgacctg ttcagcgaag agtaatcgga tgcaggcagg ggaagtgtct
    10081 gtttaccctg cctggtctga tacgagaata cgcataactt atgaagccaa tttttagccg
    10141 tggcccgtcg ctacagattc gccttattct ggcggtgctg gtggcgctcg gcattattat
    10201 tgccgacagc cgcctgggga cgttcagtca aatccgtact tatatggata ccgccgtcag
    10261 tcctttctac tttgtttcca atgctcctcg tgaattgctg gatggcgtat cgcagacgct
    10321 ggcctcgcgt gaccaattag aacttgaaaa ccgggcgtta cgtcaggaac tgttgctgaa
    10381 aaacagtgaa ctgctgatgc ttggacaata caaacaggag aacgcgcgtc tgcgcgagct
    10441 gctgggttcc ccgctgcgtc aggatgagca gaaaatggtg actcaggtta tctccacggt
    10501 taacgatcct tatagcgatc aagttgttat cgataaaggt agcgttaatg gcgtttatga
    10561 aggccagccg gtcatcagcg acaaaggtgt tgttggtcag gtggtggccg tcgctaaact
    10621 gaccagtcgc gtgctgctga tttgtgatgc gacccacgcg ctgccaatcc aggtgctgcg
    10681 caacgatatc cgcgtaattg cagccggtaa cggttgtacg gatgatttgc agcttgagca
    10741 tctgccggcg aatacggata ttcgtgttgg tgatgtgctg gtgacttccg gtctgggcgg
    10801 tcgtttcccg gaaggctatc cggtcgcggt tgtctcttcc gtaaaactcg atacccagcg
    10861 cgcttatact gtgattcagg cgcgtccgac tgcagggctg caacgtttgc gttatctgct
    10921 gctgctgtgg ggggcagatc gtaacggcgc taacccgatg acgccggaag aggtgcatcg
    10981 tgttgctaat gaacgtctga tgcagatgat gccgcaggta ttgccttcgc cagacgcgat
    11041 ggggccaaag ttacctgaac cggcaacggg gatcgctcag ccgactccgc agcaaccggc
    11101 gacaggaaat gcagctactg cgcctgctgc gccgacacag cctgctgcta atcgctctcc
    11161 acaaagggct acgccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc cgggagggca
    11221 atagtggcga gctatcgtag ccagggacgc tgggtaatct ggctctcttt cctcattgcg
    11281 ctgttgctgc aaatcatgcc ctggccggat aacctgattg ttttccggcc aaactgggtg
    11341 ttactcatct tgttgtattg gatcctggcc ttgcctcatc gcgtaaatgt gggcacaggt
    11401 tttgtgatgg gtgccatact ggatctgatc agcggctcga cgcttggcgt acgcgtattg
    11461 gcgatgagca tcattgctta cctggtggcg ctgaaatacc agcttttccg caacctcgca
    11521 ttatggcagc aggcgctggt cgtcatgttg ctttcgctgg tggtggatat tattgttttc
    11581 tgggcagagt ttttagtgat taacgtctct ttcagaccgg aagtgttctg gagtagtgta
    11641 gtcaatgggg tgctctggcc gtggattttc ttgctgatgc gcaaagtccg tcagcagttt
    11701 gcagtgcaat aaaggtttct atgacttctc tgtatttagc ttccggttct ccgcgtcgtc
    11761 aggagttact tgcgcaactt ggcgtgacct ttgaacgtat tgttacgggc attgaggagc
    11821 agcgtcagcc gcaggagagc gcgcagcagt atgttgtgcg tctggcgcgc gagaaagcac
    11881 gggcaggtgt cgcgcaaacg gcgaaggatc tcccggtgct gggtgcggat actatcgtta
    11941 tcctgaacgg agaagtgctg gagaaaccgc gcgacgcaga gcatgcggcg cagatgttgc
    12001 gcaaattatc gggtcagacc catcaggtga tgacagcagt ggcgttggcc gacagccagc
    12061 acattctcga ttgcctggtg gtcaccgatg tgactttcag aacgttaaca gacgaagaca
    12121 tcgcgggcta tgtcgccagc gatgaaccgt tagataaagc aggtgcatac ggtattcagg
    12181 ggctgggtgg ctgttttgtc aggaagataa atggcagcta tcacgccgta gtcggcttac
    12241 cgctggttga aacgtatgaa ttattaagta attttaacgc actgcgtgag aaaagggata
    12301 aacatgacgg ctgaattgtt agtaaacgta acgccttcgg aaacgcgagt ggcgtatatt
    12361 gatggcggta ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg aatagtaggc
    12421 aatatctaca agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc ttttgtagat
    12481 attgggctgg ataaagccgc gtttcttcat gcatccgaca tcatgccgca caccgaatgt
    12541 gtggcgggtg aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact ggttcgtcag
    12601 gggcaagatc tgatggtgca ggtggtgaaa gatccgcttg gcactaaagg tgcgcgcctg
    12661 accaccgata tcacgctccc ttctcgctat ctggtgttta tgccaggggc ttctcacgtt
    12721 ggggtttccc aacgtattga aagcgaatca gaacgtgaac gcctgaaaaa agtggtcgca
    12781 gagtattgcg acgagcaggg cgggtttatc atccgtaccg cagcggaagg ggttggcgag
    12841 gctgaactgg cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt tatggagcgt
    12901 aaaaaacgcc cgcagacccg ttatcagctg tacggcgaac tggcgctggc gcagcgtgtt
    12961 ctgcgtgatt tcgccgatgc cgaactggac cgcattcgcg ttgactcacg cctgacttac
    13021 gaagcgttac ttgagttcac ctcggagtac attcccgaga tgacaagcaa gctggagcat
    13081 tacacaggac gccagccgat tttcgatctc tttgatgtcg aaaacgaaat ccagcgagcg
    13141 ctggaacgca aagtagaact gaaatccggt ggttatctca ttatcgacca gaccgaagcg
    13201 atgaccaccg tggacatcaa taccggagcg tttgtcggtc atcgcaatct ggacgacacc
    13261 attttcaata ccaatattga agcgacgcag gctatcgctc gccagttacg gttgcgtaat
    13321 ctgggcggga ttatcattat tgatttcatc gatatgaata atgaagatca ccgccgccga
    13381 gtgctgcact cgctggagca ggcgttgagc aaagaccggg tgaaaaccag cgttaatggt
    13441 ttttcggcgc tggggctggt ggagatgacg cgtaaacgca cccgcgaaag cattgagcac
    13501 gtactgtgta acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac cgtggaaacg
    13561 gtatgctatg aaatcatgcg cgagattgtt cgtgtccacc atgcttacga ctccgaccgt
    13621 ttcctggtct atgcttctcc ggcagtagct gaagccttga aaggcgaaga gtcacactcg
    13681 ctggcggaag tggaaatttt cgttggcaaa caggttaaag tacaaattga accgctctat
    13741 aaccaggagc agtttgacgt cgtaatgatg taaacagatg ctgggccgcc atccggcaaa
    13801 gggtttttga gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat tgccggggat
    13861 tttactgctt actggagccg cgctcgttgt gatcgctgcc ctgctggtta gcggcctgcg
    13921 tattgcttta ccgcatcttg acgcctggcg tccggaaatc ctcaacaaaa tagaatccgc
    13981 gactggcatg ccggtagaag ccagtcagct ctcagccagc tggcagaatt ttggcccgac
    14041 gcttgaagca cacgacatcc gtgcagaact aaaagatggc ggcgaatttt cggttaaacg
    14101 cgttactctg gcgctggatg tctggcagag cctgttacat atgcgctggc agtttcgcga
    14161 cctcactttc tggcagctgc gctttcgcac caacactcct atcaccagcg gtggtagtga
    14221 tgacagtctg gaagccagtc acatcagcga tctgtttctt cgtcaatttg accatttcga
    14281 tcttcgcgac agtgaagtca gtttcctgac gccatccggt cagcgcgccg agctggcgat
    14341 cccacaactc acctggctga acgatccacg tcgacaccgt gcggaaggcc tggtaagcct
    14401 ctccagcctt accggacagc acggcgtgat gcaggtgcgc atggatttgc gcgatgatga
    14461 ggggttgtta agcaatggtc gcgtctggct ccaggcggat gacatcgacc tgaagccgtg
    14521 gctcggtaaa tggatgcagg acaatattgc gctggaaacg gcacagttct cccttgaagg
    14581 ctggatgacg atcgacaaag gcgatgtaac cggcggtgac gtctggctga aacagggcgg
    14641 tgccagctgg ttgggcgaga agcaaacgca tacgctgtcg gtggataatc tgaccgcgca
    14701 tattacgcgt gaaaatccgg gctggcagtt ctctattccc gatacacgga tcacgatgga
    14761 cggcaaaccc tggccgagcg gagcattgac gctggcctgg ataccggaac aggacgttgg
    14821 cggcaaagac aataaacgca gtgacgaact ccggattcgc gccagtaatc tggagctggc
    14881 aggcctggag ggcatacgcc cgctggccgc gaaactttca cctgcactgg gtgatgtttg
    14941 gcgctccaca caaccgagcg gcaagattaa cactctggcg ctggatatcc cgcttcaggc
    15001 ggcagacaag acccgttttc aggcatcgtg gagcgatctg gcctggaagc aatggaaatt
    15061 attaccgggt gcggaacact tctccgggac gctttccggc agcgttgaaa atggtttgct
    15121 taccgcgtcg atgaagcagg caaagatgcc ttacgaaacg gtattccgtg cgccactaga
    15181 aatcgccgac ggccaggcaa ctataagctg gctgaacaat aacaaaggtt tccagctgga
    15241 tgggcgtaat attgacgtta aagccaaagc cgtccatgcg cgcggcggtt ttcgttacct
    15301 gcaacctgct aacgatgaac cctggctggg tattctggct ggcatcagta ccgatgatgg
    15361 ttcacaagcc tggcgctatt tcccggaaaa cttgatgggt aaagacctgg ttgattactt
    15421 aagtggcgcg attcagggcg gtgaagcgga taacgcgacg ctggtttatg gtggcaatcc
    15481 gcaactcttc ccctataaac acaacgaagg tcagtttgaa gtgctggtgc cgctgcgcaa
    15541 cgcgaagttt gccttccagc cggactggcc tgcattaact aaccttgata ttgaactgga
    15601 ctttattaac gacggtttat ggatgaaaac cgatggcgtt aatctgggcg gcgtgcgcgc
    15661 gagtaatctt accgcagtga tccctgacta ctcaaaagaa aaactgctga ttgacgctga
    15721 cattaaaggt ccgggtaaag ccgttggccc ttactttgat gagacaccgc tgaaagattc
    15781 tctgggtgcg accctgcaag aactccagct cgacggcgat gtgaatgctc gcttacatct
    15841 tgatatcccg ctgaacggcg aactggtaac cgcgaaaggt gaagtgacgc tgcgtaataa
    15901 cagtctgttt atcaaaccac tcgacagcac cctgaaaaat ttgagcggta aattcagctt
    15961 tatcaatagc gatctgcaaa gtgaaccact gacagcaagc tggtttaatc agccgttgaa
    16021 cgtggatttt tccaccaaag aaggggcaaa agcctaccag gtagcggtaa acctcaacgg
    16081 taactggcaa ccggcgaaaa ccggcgttct gcctgaagcg gtgaacgaag cattgagtgg
    16141 cagcgtggcg tgggatggta aagtgggcat tgatctgcct tatcatgctg gtgcgaccta
    16201 taacatagag ctgaacggcg atctaaagaa tgtgagcagt cacttacctt caccgttagc
    16261 caaacctgcg ggtgaaccac tagcggtaaa cgttaaggtt gatggcaatc tcaacagctt
    16321 tgaattaacc ggacaggctg gtgcggataa tcatttcaat agccgctggt tgctcggtca
    16381 aaagctgacg ctcgatcgtg ctatttgggc ggcagacagt aaaacgctcc cgccgttgcc
    16441 ggaacaaagt ggcgttgaac tcaatatgcc gccgatgaat ggtgccgagt ggctggccct
    16501 gttccagaaa ggcgctgcgg agagtgtcgg tggtgcagcg agtttcccac aacacataac
    16561 gttacgtacg cctatgttgt cactgggaaa tcagcaatgg aataacctga gtattgtttc
    16621 gcaaccgacg gcaaatggca ccctggttga agcgcaaggg cgtgaaatca acgccacgct
    16681 agcgatgcgt aataacgcgc cgtggctggc gaatatcaaa tatctttatt acaacccgag
    16741 cgtggcgaaa actcgtggtg attcaacgcc gtcatcacct ttcccgacaa cggagcgcat
    16801 taacttccgt ggctggccgg acgcccaaat acgatgcaca gagtgctggt tctgggggca
    16861 aaaattcggt cgcattgaca gtgatatcac catttctggc gatacgttaa cgctgaccaa
    16921 tggactgatt gatactggtt tctcgcggct tactgccgat ggtgaatggg ttaataatcc
    16981 ggggaatgaa cgtacctcgc tgaaaggaaa actgcgcggg cagaaaattg atgccgccgc
    17041 agaatttttt ggtgtcacga cgcccatacg ccagtcgtca tttaatgtgg attacgattt
    17101 acactggcgc aaagcaccct ggcagccaga tgaagcgacg ttgaatggca tcattcatac
    17161 tcaactgggt aaaggcaaaa ttaccgaaat caataccgga catgccgggc aattgctgcg
    17221 cttattgagc gtagatgccc tgatgcgtaa gctgcgtttt gatttcagag acacttttgg
    17281 cgaagggttc tattttgact ccattcgcag caccgcgtgg attaaagacg gcgttatgca
    17341 caccgacgac acgctggtgg atggcctgga ggcggatatc gccatgaaag ggtcggtaaa
    17401 tctggtacgt cgcgacctga atatggaagc ggttgtcgca ccagagattt ctgcgacggt
    17461 gggcgtggct gcggcttttg cggttaaccc cattgttggc gcggcagtgt ttgccgccag
    17521 taaagtgctg gggccgctgt ggagcaaagt ctccattttg cgctatcaca tttcgggtcc
    17581 gctggacgat ccgcaaatca acgaagtgtt gcgccaaccg cgtaaagaaa aagcgcaatg
    17641 atttgacgag ggcgcgtaat tgccccaatc tcataggata atcgttgcca aaggccaacg
    17701 agccagaaca taaccgtagg tcggataggg cgtt
//
LOCUS       DBBGLQ010000088        17620 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.088, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000088 DBBGLQ010000000
VERSION     DBBGLQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.088"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(189..1373)
                     /locus_tag="ACIYSB_003945"
     CDS             complement(189..1373)
                     /locus_tag="ACIYSB_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5430883.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQISLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(1673..1767)
                     /locus_tag="ACIYSB_003946"
     tRNA            complement(1673..1767)
                     /locus_tag="ACIYSB_003946"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1731..1733),aa:Sec,seq:tca)
     gene            2060..3442
                     /locus_tag="ACIYSB_003947"
     CDS             2060..3442
                     /locus_tag="ACIYSB_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGC5430884.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3452..5770
                     /gene="yicI"
                     /locus_tag="ACIYSB_003948"
     CDS             3452..5770
                     /gene="yicI"
                     /locus_tag="ACIYSB_003948"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGC5430885.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRDYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5823..7532)
                     /locus_tag="ACIYSB_003949"
     CDS             complement(5823..7532)
                     /locus_tag="ACIYSB_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGC5430886.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINTEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(7653..9044)
                     /gene="xanP"
                     /locus_tag="ACIYSB_003950"
     CDS             complement(7653..9044)
                     /gene="xanP"
                     /locus_tag="ACIYSB_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGC5430887.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9324..10529
                     /gene="gltS"
                     /locus_tag="ACIYSB_003951"
     CDS             9324..10529
                     /gene="gltS"
                     /locus_tag="ACIYSB_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGC5430888.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(10698..12779)
                     /gene="recG"
                     /locus_tag="ACIYSB_003952"
     CDS             complement(10698..12779)
                     /gene="recG"
                     /locus_tag="ACIYSB_003952"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGC5430889.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(12785..13474)
                     /gene="trmH"
                     /locus_tag="ACIYSB_003953"
     CDS             complement(12785..13474)
                     /gene="trmH"
                     /locus_tag="ACIYSB_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGC5430890.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13481..15589)
                     /gene="spoT"
                     /locus_tag="ACIYSB_003954"
     CDS             complement(13481..15589)
                     /gene="spoT"
                     /locus_tag="ACIYSB_003954"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGC5430891.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(15608..15883)
                     /gene="rpoZ"
                     /locus_tag="ACIYSB_003955"
     CDS             complement(15608..15883)
                     /gene="rpoZ"
                     /locus_tag="ACIYSB_003955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGC5430892.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(15938..16561)
                     /gene="gmk"
                     /locus_tag="ACIYSB_003956"
     CDS             complement(15938..16561)
                     /gene="gmk"
                     /locus_tag="ACIYSB_003956"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGC5430893.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16819..>17620
                     /gene="ligB"
                     /locus_tag="ACIYSB_003957"
                     /gene_synonym="yicF"
     CDS             16819..>17620
                     /gene="ligB"
                     /locus_tag="ACIYSB_003957"
                     /gene_synonym="yicF"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HGC5430894.1"
                     /translation="MKVWMAILIGILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEW"
BASE COUNT         4054 a         4440 c         4693 g         4433 t
ORIGIN      
        1 gtatagaaat aactgcaagg ctgtattttg taattgccaa tttaaaaatg tcaatttgtt
       61 tttaattatg tttttgtaat aagtttgaag acacaaaaaa ataatataat aacaaaaatc
      121 ctccagcctt gtaggccaga ggataaaata cttaattgag gaaaacatca gggaaaagta
      181 tttctgactt aactttcccg gctggagagt gaatcaatgt aatcagcgta ccattgcatc
      241 atttcacggc ggccatcgag atacagggca tgattatagg tacccctgat ggaattttta
      301 tccacatgag ctaactgcat ttcaatccag gcggagttaa agccttgttc atgcagaatt
      361 gtactcatgg tgtgtctgaa accatgccct gtgagtcttc ccttatatcc aagtaattca
      421 attacttggt taatgctttc cttactaatt ggtttgcggg gatcatttct accgataaag
      481 actaggggat gatgttttga gattggttca agctgcttaa atagcataat aacctgctca
      541 gagagcggca caatatgagg tcgcttcatc ttcattactt ctgccgggat ctcccacaat
      601 tttgtctcaa aatcgatatc ttcccagcgt gcaaaacgca gttcttgtgt tcgtacgccg
      661 gttaacataa tgatctgagt agctgtctta gtgattatac ttccggtata acccgccaga
      721 tcgttgagga agtggggaag ttcattagca gtaagaaagg gaaaatgtac ttttttcggt
      781 gtagctaaag cactggcaag atcgggggct gggttatagt ctgcccggcc agtaacaatt
      841 gcatagcgga acacctcacc acagcgctgg cgtaccttgc gcattttctc cagagctcca
      901 cgtttttcca ttttacgcag tgcttccagt aactccattg gtttgatttc ggcaataggg
      961 cgcttgccaa tgtagggaaa aatgtctttt tcaaaggtat caatgatttc atcacgatag
     1021 cgtaatgacc accgatcggc ttttgactgg tgccattcgc gagcaatagc ctcgaacgta
     1081 tttttcagac ttatttgctg cgcgattttt tcttcctttt ttgcctcccc tggatcgcct
     1141 ccagcagcga ggagtttcct ggcttcactg cgtttctcgc gagcatcggc tagcgttaca
     1201 tcggggtata cgccaataga aagcatcttt tctttaccag caaagcggta cttcatgcgc
     1261 cagtatttag aaccattagt gttaatcagt agatacatac cgccgccatc agcaagctta
     1321 tagggtttgt ctttgggttt tgcggttttc actttgatat cggttaatgc catgacatcc
     1381 tccggctttt gatgaattca tgttcctgag ggggatttct gccagtaact aacctgatac
     1441 ccccaataca ccccttgtat aacaaagatg ttgttaaaag ccgttgttat gtatcacatt
     1501 tttcctatta tgggggtgct tgtatttcaa taggaaataa caccccctaa agtaccccca
     1561 aaagaaactt gatacggggt gaagtaggtt gacctcagga tacaaataaa tactgatata
     1621 caagggatta gaagagattt attgacattg ggagagttca gaagaggtaa actggcggaa
     1681 gatcacagga gtcgaacctg cccgggaccg ctggcggccc caactggatt tgaagtccag
     1741 ccgcctcacc ggagacgacg atcttccgcg cctcgattgc tacatggagg cggggcgcat
     1801 tatagctact tccttgagtt tctacatccc ccagatcagt tttttccgcc taatacacgc
     1861 agcgtattat cacgaaagca ttatagttca tataaaaccc atattttaca atgatatatg
     1921 agcggaatta acgctaccac gatcacataa aacaagaatc ataaattaat aaccagatat
     1981 cggaatattc gctctcacag ggatggttta caaaatgcgt tatcggtctc agcaccccta
     2041 tagcatcaag gaaaagcaga tgaagagtga agtgttgtcc gttaaagaga aaattggtta
     2101 tggcatggga gacgccgcca gccacattat tttcgataac gtaatgttat atatgatgtt
     2161 cttttatacc gatatttttg gcattcctgc cggttttgtc ggaaccatgt ttttggtcgc
     2221 tcgtgcactg gatgcgattt ccgatccttg catggggttg ttggccgatc gaacgcgctc
     2281 tcgctggggt aaatttcgtc cgtgggtact gtttggcgca ctgccattcg ggatcgtctg
     2341 tgtactggcc tatagcacgc cagatctcag tatgaacggc aaaatgatct atgcagcaat
     2401 tacttacacc ctacttacct tactttatac cgtcgtcaat atcccttact gcgcattggg
     2461 tggtgtaatc accaatgacc cgactcagcg tatctcgctg caatcctggc gttttgtgct
     2521 ggcgaccgcg ggaggcatgc tttctactgt tctgatgatg ccactggtta atttaattgg
     2581 cggtgataat aaaccactcg gtttccaggg cggtatcgcg gtcctttccg tggtggcatt
     2641 catgatgctg gcattttgtt tcttcaccac taaagaacgc gttgaagcac cacctacaac
     2701 aacgtctatg cgggaagatt tacgtgatat ctggcaaaac gaccagtggc ggattgtcgg
     2761 tttactaacc attttcaata tcctggcggt gtgcgtacgc ggtggggcga tgatgtatta
     2821 cgtcacatgg attttgggca cgccggaagt gtttgtcgct tttctcacca cttattgcgt
     2881 gggtaacctg attggttccg cactggcaaa acctctgacc gactggaaat gtaaagtcac
     2941 tatcttctgg tggacgaacg ccctgctggc agtgattagc ctcgcgatgt tctttgttcc
     3001 catgcaggcc agcatcacta tgtttgtctt catcttcgtg attggtgtgt tgcatcaact
     3061 ggtgacacct atccagtggg taatgatgtc cgataccgtc gactacggcg agtggtgcaa
     3121 tggtaaacgc ctgaccggga tcagttttgc tggcacgctg tttgtgctca aactggggtt
     3181 ggccttcggc ggcgctctta tcggctggat gctggcttat ggcggatatg atgcggcaga
     3241 aaaagcgcag aacagcgcca cgattagcat cattattgcg ctattcacga ttgttccggc
     3301 gatctgttat ttgctgagcg cgattatcgc taaacgctac tactcactca cgacgcacaa
     3361 tctgaaaacc gttatggaac agctggctca gggtaaacgc cgttgccagc aacaattcac
     3421 ctctcaagaa gtgcagaact aaggaacggc aatgaaaatt agcgatggaa actggttgat
     3481 tcaacctggc ctcaatttga ttcacccgct tcaggtgttc gaggttgaac agcaggataa
     3541 tgaaatggtg gtctatgctg ccccccgtga tgtgcgtgaa cgtacctggc agcttgatac
     3601 gcctttattt acgttgcgct ttttctcccc acaggaaggt attgtcggtg tgcggattga
     3661 gcattttcag ggggcgctga ataacggtcc tcattatccg ctcaatattt tgcaggacgt
     3721 gaaggtcaca atcgaaaaca cagaacgtta tgctgagttt aaaagtggca acttaagcgc
     3781 gcgtgtcagc aaaggtgagt tctggtcact ggattttctg cgcaacggcg aacgtattac
     3841 cggtagtcag gtgaaaaata atggctacgt gcaggacacg aataatcaac gcaattatat
     3901 gtttgagcgg cttgatcttg gcgttggcga aacagtttac ggtctgggag agcgctttac
     3961 tgccctggtg cgcaatggcc agacggtaga gacctggaac cgggacggcg gcacaagtac
     4021 tgaacaggcg tataaaaata tcccgttcta catgactaac cgtgattatg gggtactggt
     4081 caatcatccc cagtgtgtct cttttgaagt gggatcggag aaagtctcca aagtgcagtt
     4141 cagcgttgag agtgaatatc tcgaatactt tgttatcgac ggcccgacgc cgaaagcggt
     4201 acttgatcgt tatacccgct ttactggtcg tccggcgctg ccgcccgcgt ggtccttcgg
     4261 cctgtggcta accacttcat ttaccaccaa ctacgacgaa gcgacggtaa acagctttat
     4321 cgatggtatg gcggaacgca atctgccgct gcatgttttc cactttgact gtttctggat
     4381 gaaagccttc cagtggtgcg attttgagtg ggacccgctg actttccctg acccggaagg
     4441 gatgatccgc cgcctgaaag cgaaaggact gaaaatctgc gtctggatta acccctatat
     4501 cggtcaaaaa tcccccgtct ttaaagagtt acaagagaaa ggctatttac tcaaacgccc
     4561 ggacggttcg ctatggcagt gggataaatg gcagccaggt ctggcgattt atgactttac
     4621 caatccggat gcctgcaaat ggtacgccga caaactgaaa ggtctggtcg cgatgggcgt
     4681 tgattgcttt aagaccgact ttggcgaacg tatcccaact gatgttcagt ggtttgacgg
     4741 ttccgatccg cagaaaatgc ataaccatta tgcgtacatc tacaacgaac tggtgtggaa
     4801 cgtgctcaag gacaccgttg gtgaggaaga agctgtcttg tttgcccgct cggcctccgt
     4861 cggtgcgcag aaattcccgg tacactgggg tggcgattgt tacgctaact acgaatcaat
     4921 ggcggaaagc ctgcgcggtg gtttgtctat tggcctttca ggttttggct tctggagcca
     4981 cgatatcggc ggctttgaaa ataccgctcc ggcgcacgtt tacaaacgct ggtgcgcgtt
     5041 tggtttgctc tccagccata gccgtttaca cggtagcaaa tcttatcgtg tgccgtgggc
     5101 ctacgatgat gagtcctgtg atgtggtgcg cttcttcacg caactgaaat gccgcatgat
     5161 gccgtatctg tatcgtgaag ctgcgcgtgc gaacgcgcgg ggtacgccga tgatgcgggc
     5221 catgatgatg gagttcccgg acgatccggc ttgtgattac cttgaccgtc aatacatgtt
     5281 aggcgacaac gtgatggttg cgccggtgtt cactgaagcg ggcgatgtgc agttctacct
     5341 gccggaaggt cgctggacac acctgtggca caacgatgaa ctcgacggta gtcgctggca
     5401 taaacagcag cacggcttcc tgagtctgcc cgtttatgtg cgtgataaca ctctactggc
     5461 gctgggcaac aacgatcaac gtcccgatta cgtgtggcac gaaggcacgg cattccacct
     5521 ctttaatctg caagacgggc atgaagccgt ctgtgaagtg cccgctgctg acggatcggt
     5581 gatctttact ttaaaagcag cacgtactgg caacacgatt actgtgactg gtgcgggcga
     5641 ggcgaagaac tggacactgt gcctgcgcaa tgttgtgaaa gtaaatggtc tgcaagacgg
     5701 ttcgcaggct gaaagtgagc aggggctggt ggtgaagcct caagggaatg cgctgacaat
     5761 tacgttgtaa tctattgatg gaatgccggg tacgtctgag tcgtacccgg cgagattgat
     5821 gtttaccgca ccggctccgt cgatgtaact tcaccgctgg aaacgatgcc agcattcatg
     5881 gtttgagtca cttgctgctt tgcggcgttc acggcatgca attgcccgct aaccgtaggt
     5941 ttcaacggga cattggcctg aatatcgcca ctggcggtaa gctgaatatt gccgtcgcca
     6001 gtcaacggta attcaggcca tccccactgt tgcaaaatat tcaccggcac accgcgtcca
     6061 ttcaggctga tatgtgtctg acgttgtggc gtttgtgaaa cactggcggt ggcttccaga
     6121 atgccttttt cagtaaatgc actcagttcg ctgatattca ccgtgctgct gttggcggtc
     6181 agcgccagcg acgggcgacg aacatcaaca cgattgaatg tcgcggcggc ggcattcaga
     6241 ttcgccgagc cactccagac gccccattta tgatcggtaa ccagcgtcag gttggcaccg
     6301 taaccatcga gcgcggtgag ctgccacggg aagtcagggt cgatatcaat gatcagattg
     6361 cggctggcgc taaatctctt cagttgcagg ctgtttaacc aaccgggtgt cgtttccatc
     6421 cacaactgtt gccagttttt cggcaaggta tattccagcc cggcaattgc cgcatcatca
     6481 aggatcaacg ttttcccgtc acgcagccag ttccctgacg ttctgaccat acccccttcc
     6541 cagcggctgg tgaactggcg cagcgctacg ccctgcgggg aaaattccgt gtttataatc
     6601 gggtcaaata aatgcagcga accataaatg aactcgctag cgttcatcga cagtttgcct
     6661 tcctgtgtct gccagtcatc tttactgaag gtcatgttgc gcaagctgag atcgaggtcg
     6721 gtcaccgccc agtccggacc ttgcaaacga gcatcgatca cttccaggcg accaatctgc
     6781 aacgacggga cagagcgtaa tggcgcaaag aagtcggtta gcgatttttc gctttgtaga
     6841 cgtatatccg ccatgcgcag gttttccact tgccagctgc cgtcggcgtt acgctgcgcg
     6901 tttccggtta atgtcccgcg ggcgatgtcg gcacccaggt tagtcagcgt aacgcgatcg
     6961 ttatcaatac tgccttcaat cagtacattg gtggcaggaa catcattgag cgaaagcgat
     7021 ccggcactaa actgaatctg cgccttcgta cccagcactt taccggcttt tggtgaccac
     7081 ggaactacgc cgccatttac ccgctgcgcg ctcagtttcc attcgctatt cgggctatta
     7141 aacgccatat cacgcagttg cagacgatcg gctttgaacg gtagcggcgc ggtctggtcg
     7201 gtgagattca gcgtcccgtt ttccagcagg atggtatcga catggcgtgg ttcggtcagt
     7261 tgccgactgc ttagcgcaat gtcgacactt ttggccacca gggtcgcggg ctggccatca
     7321 cgaccaaacg tgacgttctc cagcacgata tgagatggcg cggaaaaacg gtgatccatc
     7381 gccccgaagg ccagatgata gtcgctattc tcggaaaccc atgcgctgat atgttctgct
     7441 ccccagcggg tttgcagaag aaaatagagg ccagcgatcg ccactaacag agcgatgaga
     7501 atgtagagaa gcagcttccc aataaatttc atggtcttcc atcccgtgaa atgcacataa
     7561 gggagttatg cacgatttac gcgcaatcct caaggcggga atggttaaag aggtgagact
     7621 gcggcggtaa ttatcttacc gccgccagtg aattactgtt tttctggtgg gaaaatcaga
     7681 ttcagcacga tggcagtaat accgcccgcg gcgatcccgg aggagagcag gtttttcagc
     7741 cattcagggg caaactgcaa aatcagcggc tgctgagaca cgcccagacc aaccgccagc
     7801 gacagcgcga taatcagaat cgcccgacgg ttcagcggct cacgagaaac gatacgcaca
     7861 ccggaggcgg cgatggtgcc aaacattaca agcgttgcgc cgcccagaac cggttctgga
     7921 atgtgttgta caaaaccgct cactgccggg aacagaccca gcacgatcaa catcagcgcg
     7981 acgacaaaac cgacatagcg gctggcaaca ccagtcaact ggatcactcc gttgttttgc
     8041 ccgaagcagg agttcgggaa ggtgttaaac accgccgaaa caaacgagtt caggccgttt
     8101 gccagcacgc cgcctttcag gcgtttcatg tacagcggac cggacactgg ctgttcggaa
     8161 acgtcagagg tcgccgtgat atcgccaatg gtttccagcg aagtgatcat aaagaccagc
     8221 atcagcggca gcagcagact ccattcaatg ccaagaccgt aatagagcgg cgttggcacc
     8281 ataatcagtt cttgcgtcat aggttcgttg ctttctggca acatgcccat aaaccacgcc
     8341 agcgcatatc cggccgccat cgcaattacc agtgaggcca cgcgtaagta agggttacgt
     8401 tgacggttaa gcaggataat taaggctaag accacgcctg ccagcagcag atttttcggt
     8461 gcgccgaagg tgttatcgct catggctgcg taaccgccgc caatggacgt taacccaacc
     8521 tgaattagcg acaggccgat aatcatcacc acaacgccag aaaccagcgg cgtaataatg
     8581 cggcgcgcca gatgcagaac gcgggagatc accatctcgg tgcaacttgc cagcatcaac
     8641 gtgccgaaca aagccgccat catggtagga acatcagcac caccggtttt cagcgcggta
     8701 ccgcccataa tcagcggggc aacaaagttg aagctggtgc cctgaataga caacagcccg
     8761 gagccaaccg gaccccaggc cttaatttga ataatcgatg ccacaccgga ggcaaacagc
     8821 gacatactaa taatgtgttg cgtgtcttgt gccggtaaac ccagcgcctg gcagattaat
     8881 agcgctggcg tgatcaccgc aacgaacatc gccagcagat gctgacaggc ggcaaacagg
     8941 gtttgaggaa gcggcggacg atcttcaaga cggtaaatca gttcgctgtt ttgagtctgc
     9001 gcaaccggtt gcgcattttc tgactcgagg gtggaaacag acatcgaaaa caatcccgtg
     9061 gtagcaaagc gggcatttta gctgaccgaa agagaaaagc aaacgtttgc cacatcctca
     9121 tctaaatatt taaatgagat aaatgcactt ttttatataa cttttggtat ttttctgctt
     9181 aaaatccatg ccatttgttc attaacagct gagaatttga cccaggaaag cataactcga
     9241 tacccgtcat agttcatgaa gttgccactg cgcgatgaag tatgacgagt atgaaagagt
     9301 gatgcggata caaaggagta actatgtttc atctcgatac tttagcaacg cttgttgccg
     9361 caacgctgac gttgctgctc gggcgtaagt tggtccattc cgtctccttt ttgaagaaat
     9421 acaccatacc ggaacctgtt gcgggtggtt tgttggtggc gctggcgcta ctagtactga
     9481 aaaaaagcat gggctgggaa gtcaactttg atatgtccct gcgcgatccg ttaatgctgg
     9541 ctttcttcgc caccattggc ctgaacgcca acattgccag tttgcgtgcc ggtgggcgtg
     9601 tggttggcat cttcttgatt gtggttgttg gtctgttggt gatgcaaaat gccattggca
     9661 ttggtatggc tagcttgtta gggcttgatc cgctgatggg gctgttggcc ggttctatta
     9721 cgctttccgg cggtcacggt acgggcgctg cgtggagtaa attgttcatt gaacgttatg
     9781 gcttcaccaa tgcgacggaa gtggcgatgg cctgtgcaac gttcggtctg gtgctgggcg
     9841 gcttgattgg cggtccggtg gcgcgctatc tggtgaaaca ctccaccacg ccgaacggta
     9901 ttccggatga ccaggaagtc ccgacggcgt ttgaaaagcc ggatgtggga cgcatgatca
     9961 cctcgttggt gctgattgaa actatcgcgc tgattgctat ctgcctgacg gtggggaaaa
    10021 ttgttgcgca acttttggct ggcactgctt ttgaactgcc gaccttcgtc tgtgtactgt
    10081 ttgttggcgt gattctgagc aacggtctgt caataatggg cttttaccgc gtctttgagc
    10141 gtgcggtatc cgtgctgggt aacgtaagct tgtcgttgtt cctggcgatg gcgttgatgg
    10201 ggctgaaact gtgggagctg gcttcgctgg cgctgccgat gctggcgatt ctggtggtac
    10261 agaccatctt catggcgttg tatgccatct tcgttacctg gcgcatgatg ggcaaaaact
    10321 acgatgcggc agtgctggct gcgggtcact gtggttttgg cctcggtgca acgccaacgg
    10381 caatcgccaa catgcaggcg atcactgaac gctttggccc gtcgcacatg gcgtttttgg
    10441 tggtgccgat ggtcggtgcg ttctttatcg atatcgtcaa tgcgctggta attaagttgt
    10501 atttgatgtt gccgattttt gccggttaac cgatgaagcg gcggtagaag tgccgccgca
    10561 acaaagacaa atgcctgata cgcttcgctt atcaagcctg cgtattgatt cataatttat
    10621 tgaatttgta gggtgatgaa tcgcatccgg caggaaggta gggtaacctg aaatggcggt
    10681 cttctcactg ccgcctttta cgcattcgag taacgttccg tctccggcat ccagcgttct
    10741 atcagggctt ttgcctgttg tgggtaacgt tcgtgaatat ggcgtgccag gcgctgaact
    10801 tccgggatca tcgcctgatc gcgcagtaaa tccgccactt taaattcagc attacccgtc
    10861 tgacgcgtgc ctaacaattc gccagggccg cgaatctcca gatctttttg cgcaatcaca
    10921 aaaccgtcgt tactgtcgcg cagcacttgc aggcgaattt gcgccgtttt agaaagcggc
    10981 gttttgtaga gcagcacgca gtgagaagcc accgcgccac gacctacgcg cccgcgcagc
    11041 tggtgtaact gcgccagacc cagacgctcc gggttttcga taatcatcag actggcgtta
    11101 ggcacatcaa cgccgacttc aataacggtt gtggcaacca gcaggtgtag ctcaccttgt
    11161 ttaaacgacg ccatcaccgc ctgtttctcg gcaggtttca tccgcccgtg taccaggcca
    11221 acgttcaact ctggtagcgc cagtttcaac tcttcccagg tagcttccgc cgcctgcgct
    11281 tccagcaatt ccgactcttc aatcaacgta caaacccagt atgcctgacg accttcagtt
    11341 atgcaggcgt ggtgcacgcg gtcaatgatg tcggtacggc gggtatcagg aatagcgacc
    11401 gtagtcactg gcgtgcggcc tggcggcagc tcatctatca ccgaggtatc gagatcggca
    11461 tacgcagtca ttgccagcgt gcgggggatc ggcgtggcgg tcatgatcaa ctgatgcgga
    11521 tggaagccct gctgctggcc tttctcccac aatgccagac gctgatgcac gccaaaacga
    11581 tgctgttcgt cgataatcac cagcgccagg ccgttaaact gcacctgttc ctggaagatg
    11641 gcgtgtgtac cgacaatcat ctgcacctga ccgctggcga tggcttcctg ctgtgccagc
    11701 cgtgctttac ctttctgctt accggcgagc cagcccactt cgataccgag cggtgcaaac
    11761 cagttgcgga agttattggc gtgctgctcg gcgagtaatt cggttggtgc catcaatgct
    11821 acctgtttgc cgtgggcaat cgcacgcaac gcggcgaggg cggcgaccag cgttttaccg
    11881 gaacctacat cgccctgcac cagacgcatc atcggcacat ccagcgccat atcgcgctcg
    11941 atctccgcca ctacgcgtgc ctgtgcgccc gttggcttga acggtaaggc ggcgaggagt
    12001 ttatttttca gcgtgtcatt ggcgctcagc ggctgggcat gaaaacgctg tgctccggca
    12061 cgtaaggcta acatgctgag gttgtgcgcc agcagttctt ccagaatcag acgacgttgc
    12121 gccggatgct gcccggtttc cagatcgcta agctgtagcg tcggtggcgg gcggtgcaaa
    12181 gtgcgcaacg cttccggtag cgtcatcatt ccttgtgaca gttccggcgg caggagttct
    12241 tcaatggcgc aggtgtcgag cagatccagc gcctggtcgg ttaatttacg cagcgtggcc
    12301 tgctttacgc cttccgttgt tggataaacc ggcgtgagcg tttcctgtaa ttctggcgtg
    12361 ctgagatcgc cctgcacgcg gtattccggg tggatcatct ccgcaccata tttaccgcgc
    12421 tttgcttcgc cataagccag tacacggcgg cccgccgcca ggctattttt cattgccgcg
    12481 ctgaaattga aaaagcgcat ggtgaggatg ccggaaccgt cgctgatctg gcaggtcatc
    12541 atccgccgac cgccgaagga gatattgcag ttcagcactt cgccttccac cgtggcataa
    12601 acgcccggca gtagttctcc gatggggtag agatgggtgc gatcttcgta gcgcagggga
    12661 aggtgtaaga gtagatcctg cacggtatgc aggttgattt tcgccagttt gttactaagt
    12721 gctgcgccaa cgcccgttag ggaactgagt gggacagcat ctaacaggcg acctttcatg
    12781 gcacttaccc tgcagcctgc atagtagccc accagtcggc atcagcttcg atctcgcctt
    12841 gctgattgac gtggggataa ggcaggcctt tgcgttttgc gactttcgcc agcaccggat
    12901 agccgccttc aaacaacagg cgttgttgct ctgcttccgg caacatgctg ttttcacgca
    12961 ggtacatgcc tgcattttgc cgctgacgct gggcttcgta aagaatgagg gctgaggcaa
    13021 cggaaacatt cagcgactgc accatgccga tcatcggaat gatgatgtcc tgatccgcca
    13081 gggccaatgc ttcctgcgtg atgcccgttt tctcctgtcc catcaaaatg caggtcgggc
    13141 gagtgtaatc aatttcgcgg aaatcgacag cgttatcaga aagatgggtt gccagaatct
    13201 gcatgccctg gcctttgaga tgagcgacgg catcgccaat ggtgcggtgt gttttcacct
    13261 gtacccagct gttactaccc gccgctgccg aagccatggt gcgcatgcgg ctaccaggcc
    13321 agacggcgtg aacttcatgt acgccaacgg catctgcggt acgaataatc gcagaaacgt
    13381 tatgaggttt gtggacctgc tccatgcaga cggtcagatc aggctgccgc ctggcgagca
    13441 tttcgcagat gcgtgcataa cgtgttgggt tcataaaaca ttaatttcgg tttcgggtga
    13501 ctttaatcac gtctggcatc acgcggattt tgcgcatgat attcgccaga tgcacacggt
    13561 cacgagcggt cagacgaata aaggcgctgt agacgcgacc atctttctct tccgtattca
    13621 aactttgaat attcgaagtc gtggtgttaa ttgccgccgt caggtttgcc agcgcaccct
    13681 gatgattgaa catctccacc ttgatttcgg tgatgaactc ctgcgccgtc tctttatccc
    13741 attccacagc cataaacttc tctggctctt tctggtagcc acggatatta cggcaggatt
    13801 catggtggat caccagacct ttaccggggc tgacgtgggc gataatcggg tcgccaggaa
    13861 tagggcggca gcatttcgca aaggtgatca gcacgccatc ggcaccttta atgggcagat
    13921 gtccgtggct ttgggttgcc ggtggaatgg aggcgtcccc atgttgcaga tttttcgcga
    13981 ccaccacgct cattgcgtta ccaagtccga tttctgccag cagatcgtca agcgttgcca
    14041 gcttcatgcg atccagctcg cgctgaatat tttcctgcgg gatttcattc agcttacggc
    14101 taccacccaa agcatggttg agcagacgac ggcccaggct tacagaatca tcacgcttga
    14161 ggtttttcag caactgacga attttggcgc gcgctttcga gctaacgaca aagttcagcc
    14221 aagcggcatt cgggcgagcg cccggagcgg taatgatttc aacggtttga ccgctggtaa
    14281 gcggctgcga cagcgggtaa ggctggcggt caacgcgtgc gcccacgcag gcatgaccga
    14341 tatcggtatg cactgcataa gcgaagtcga cgggcgttgc accggcaggc agctcgacaa
    14401 tgcgcccttc cggtgtgaaa acgtaaatct catccgggaa gagatcggat ttaacgctct
    14461 cgataaattc aaacgaacta ccggcgctct gttgcagctc cagcaggctt tgcatccagc
    14521 gctgggcgcg gatttgtgcg gtagtactgg tttcgccgtg ctctttataa gcccagtgcg
    14581 cggcaacacc catctccgcc atctggtcca tatcttcggt acggatctgg acctcaaccg
    14641 gcacaccgtg cgggccgatc atcgaggtgt gcaaagactg atagccgttc gcttttggaa
    14701 tggcgatata gtctttcacg cggcccggac gcggcttgta caggctgtgc atctggccca
    14761 gcacgcgata acaggtgtca gaatcattga cgatcacgcg gaaagcgtag atgtccatga
    14821 tcgagtgaaa acgctgctct ttgagcacca ttttgcagta aatcgaataa agatgcttct
    14881 cgcgaccact gacgcggcac ggtattcccg cttcctgcaa acgcccttcg atttcagaaa
    14941 gaatcttctg gatcatctct ttacggttgc cgcgcgcggc tttcaccact tctttgatta
    15001 cgcgataacg gttgggatac agcgcctcaa aacccagctc ttcgagttcg gttttaatgt
    15061 ggtggatacc taaacggtgc gccagcgggc tataaatttc gagagtttca cgggcgatgc
    15121 ggcgacgttt gtccgggcga agtgagccca gcgtgcgcat gttgtgggta cggtcggcaa
    15181 gtttgatgag gatgacgcgg atatcctgca ccatcgccat aatcatcttg cgaaagtttt
    15241 cggcctgcgc ctctttctta tcgcggaact tgagtttatc aagtttcgac accccctcta
    15301 ccagctcggc gacgctttta ccaaaaagct gttccatatc ctggtaggtg gcgggagtat
    15361 cttcaatcac gtcatgcagc agcgccgcca tcagcgtttc atagtcgagt ttcatctcgg
    15421 ccagaatgca ggcaaccgct accgggtgcg tgatataggg ttcaccgctt gaacgtgttt
    15481 gcccctcgtg agcatcacgt gcaacgagat acgcctgccg cagacgcttg atttggtctt
    15541 ccggcaggta ggtttgaatc agttgattca ggctttcaaa cagatacaag ggcgacccgc
    15601 tttgtgatta acgacgacct tcagcaatag cggtaacggc ttgtaattca gcggcttcct
    15661 gctcttgctg ttcctggcgt tcgcgaacgt cgaggatctg gttgttgatc agaccttctt
    15721 cgatttcgcg cagcgcgatt acagtggttt tatcgttttc ttccggtacc agcggatcct
    15781 ttccgcctac ctgcatctga cgagcgcgac gcgcggcgac cagtaccagg tcaaaacggt
    15841 taccaatttt ctctacagcg tcctgaacag ttacgcgtgc catacttaaa aagctccaca
    15901 ggtgaagaaa tgactgggca tgatactgaa atcaggttca gtctgccaac aatttgctga
    15961 ttaaagcgtc atgacgctgc ttttggcggc tcatgcgcag acgttcggcg cgaataatgg
    16021 tcttcaaatc ggtcaacgcg gtatcgaagt catcattcac aatcagataa tcatattcgg
    16081 cgtaatggct catttctgca acagcttgcg ccatacgctt tgcaatgacc tcttcgctgt
    16141 cctgaccgcg accgcgtaga cggcggtcca gttcaatttt ggacggcggt aaaataaaga
    16201 tactccgcgc gtgcggcatc ttctggcgaa tttgctgcgc gccctgccag tcgatatcga
    16261 gaaaaacatc gacaccggtc gccagtactt gctcaatggc ctcacgcgaa gtgccatagt
    16321 aattaccaaa aacttctgcg tgttcgagga acgcatctct gctaatcatt tctttaaatt
    16381 catcatgatt aacaaagaaa taatgttcac cgtggacttc accaggacgc ggttggcgtg
    16441 tggtgtgtga aacagaaacc tgggtgtcat acaacggttg ggtttttaat aaagcctgaa
    16501 tcaggctgga tttacccgcg ccactggggg cagaaacaat ataaagcgtg ccttgagcca
    16561 tgagtatctt tcgtatgtga ttagcgaaat aaagcctaca tacgagctta ttatacacgg
    16621 cgcggccatg tgacgtagcc cttgctcaca ctttttgcgc aaagttgttg cgttttgcgt
    16681 agctgcttcc gcatttgcct gtcattcact gcaataaaga cgattttcag gaagcgtcct
    16741 cgcagactcc gtttttgcca ctcggctttg ctttcatcac gttgtatatc tgcctgacta
    16801 tctacagcgg aggtgatgat gaaagtatgg atggcgatat taataggtat cttgtgctgg
    16861 caatcatctg tgtgggcggt ctgtccggcc tggtcgccag ccagagcaca ggaagaaatt
    16921 tcccgcctgc aacagcaaat aaaacagtgg gacgatgact actggaagga aggaaaaagt
    16981 gaggtggaag acggtgttta cgatcagtta agcgcccgtc ttacgcagtg gcaacgctgc
    17041 tttgggagcg agccccgcga cgtgatgatg ccgcctttaa atggtgcggt tatgcatccg
    17101 gttgcccata cgggtgtacg caaaatggtg gataaaaacg cattaagtct gtggatgcga
    17161 gaacgtagcg atctttgggt gcagccaaaa gttgatggcg ttgcggtaac cctggtttat
    17221 cgggacggga aactgaacaa agcaatcagt cgcggtaatg gcctgaaagg cgaggactgg
    17281 acgcagaaag ttagcttaat ttccgctgtg ccgcaaaccg ttagcgggcc tttagccaac
    17341 agtacgcttc agggggaaat atttctccag cgcgaggggc atatccaaca acaaatgggg
    17401 ggaataaatg cccgcgcaaa agttgctggc ttgatgatgc gccaggacga tagcgacacg
    17461 ctgaattctc tgggcgtttt tgtctgggca tggccggatg gaccgcagtt aatgtctgat
    17521 cgtttaaaag agctggctac cgcaggtttt actctaacgc agacgtatac ccgtgcggtg
    17581 aaaaatgctg atgaagttgc gcgcgtacgc aatgagtggt
//
LOCUS       DBBGLQ010000089        17514 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.089, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000089 DBBGLQ010000000
VERSION     DBBGLQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.089"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(42..392)
                     /locus_tag="ACIYSB_003958"
     CDS             complement(42..392)
                     /locus_tag="ACIYSB_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGC5430895.1"
                     /translation="MPPRTPKACRVRGCRNTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLQRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            complement(439..924)
                     /locus_tag="ACIYSB_003959"
     CDS             complement(439..924)
                     /locus_tag="ACIYSB_003959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430896.1"
                     /translation="MDWNMWSAIGACGSAIASLWALCYARKALNTWNRQEQFKVKLEF
                     KRALLELEDAFEAMPDNWNSTQYRIARTRVEQQYNAVVHRVDDAAQLYFKKENLKSAY
                     QNAVRAWVLCEEGIKDKSIHAEWKQLRTDYSQYIMTGGNKKCYLSKIGKIYSRIVVFI
                     D"
     gene            complement(1081..1515)
                     /locus_tag="ACIYSB_003960"
     CDS             complement(1081..1515)
                     /locus_tag="ACIYSB_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HGC5430897.1"
                     /translation="MKMSYWALILTFIACVAGGLVWSANHYHGKFLEEQKRADAAEQR
                     ADSTEAITANVLRTMAITNIIQEANQHAKQQIALESQRTQEDIKVAVADDDCASRPVP
                     AAAADRLRKYADSLRNSTSNSASSYSDFGNASTAHTRYSDLW"
     gene            complement(1512..2126)
                     /locus_tag="ACIYSB_003961"
     CDS             complement(1512..2126)
                     /locus_tag="ACIYSB_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HGC5430898.1"
                     /translation="MNQQLFQKAAGISAGLAARWFPHIDAAMKEFGITAPADQAMFIA
                     QVGHESMGFSAVVENFNYTPSALVATFGKRITQQQADALGRTSGHAARQDAIANLVYS
                     NRLGNKAPGDGWKYRGRGLIQITGLHNYRICGAALKLDLVTSPEQLEQELQAARSAAW
                     FYTSKGCMIYGADINRVTRIINGGLNGIEDRKVRYNKARAALLV"
     gene            complement(2126..2407)
                     /locus_tag="ACIYSB_003962"
     CDS             complement(2126..2407)
                     /locus_tag="ACIYSB_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGC5430899.1"
                     /translation="MELNDPTATINALLCACVVITLMFYRRGDSRHRPWVSRLAWLIT
                     VTYSAVPLAYLCGIYPHSSWPIIVANTIFLSVLVAVRGNVARLVDHLRH"
     gene            complement(2394..2780)
                     /locus_tag="ACIYSB_003963"
     CDS             complement(2394..2780)
                     /locus_tag="ACIYSB_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGC5430900.1"
                     /translation="MSDPISGSGLAGGVLTGASIYGLLTGTDYGVVFGAFAGAVFYIA
                     TAADLSASRRLAYFIVSYIAGILCSGLVGSKLANLTGYSDKPLDAIGAVIVSALAVKI
                     LTFLNNQDIGSLVALITRRGGSGGAK"
     gene            complement(2929..3477)
                     /locus_tag="ACIYSB_003964"
     CDS             complement(2929..3477)
                     /locus_tag="ACIYSB_003964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430901.1"
                     /translation="MELTRENVALLAALGVFVGTVISNVMTYLIHYSKQKNEWVKENK
                     KKKIEKAEELYRNLVLWKKSVFQTHSDWVLLVGGNLSIEQTLDKTIERNLNTPEFCKI
                     SELSSILAGIYFPDVALQIKKAQKELKPANDIYFSIMNGSRPKNINEAIATILDAGGE
                     FDKSVDKILEGLSCEISEMMSK"
     gene            complement(3617..4369)
                     /locus_tag="ACIYSB_003965"
     CDS             complement(3617..4369)
                     /locus_tag="ACIYSB_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGC5430902.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEVQLRKVTK"
     gene            complement(4383..5372)
                     /locus_tag="ACIYSB_003966"
     CDS             complement(4383..5372)
                     /locus_tag="ACIYSB_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGC5430903.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(5380..6177)
                     /locus_tag="ACIYSB_003967"
     CDS             complement(5380..6177)
                     /locus_tag="ACIYSB_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGC5430904.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPAGAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(6197..6586)
                     /locus_tag="ACIYSB_003968"
     CDS             complement(6197..6586)
                     /locus_tag="ACIYSB_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGC5430905.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISTAGRKFQSAACAAI
                     IEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDRQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            complement(6583..7236)
                     /locus_tag="ACIYSB_003969"
     CDS             complement(6583..7236)
                     /locus_tag="ACIYSB_003969"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGC5430906.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDSIFWGINTMFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGKYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            complement(7251..7334)
                     /locus_tag="ACIYSB_003970"
                     /pseudo
     CDS             complement(7251..7334)
                     /locus_tag="ACIYSB_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7331..8149)
                     /locus_tag="ACIYSB_003971"
     CDS             complement(7331..8149)
                     /locus_tag="ACIYSB_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5430907.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSLGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASVDESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(8146..8370)
                     /locus_tag="ACIYSB_003972"
     CDS             complement(8146..8370)
                     /locus_tag="ACIYSB_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430908.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            complement(8367..9257)
                     /locus_tag="ACIYSB_003973"
     CDS             complement(8367..9257)
                     /locus_tag="ACIYSB_003973"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGC5430909.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAILGRYTVQAPYKAGAGIG
                     VLEKATAYNRVDAVFLYALFCHAQIMVGRTGASQDAPVRVAAGNANSVRLTTSMIGVL
                     CGEFFKLATEAAIMATIPTFVHPEITIINGRAVTTSLAVANYFTKRHERVLDRIRNLE
                     CSAEFAEHNFVLSGYTDASGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFNQ
                     MEKQLSKPSVPSDVAHNASVLYSYISSIHQVWLQQLYPMLEKAESPLAVSLYDRINDA
                     AFLARLIHSSLNSSEVRGRK"
     gene            complement(9187..9366)
                     /locus_tag="ACIYSB_003974"
     CDS             complement(9187..9366)
                     /locus_tag="ACIYSB_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGC5430910.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            complement(9512..10096)
                     /locus_tag="ACIYSB_003975"
     CDS             complement(9512..10096)
                     /locus_tag="ACIYSB_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfL family putative regulatory protein"
                     /protein_id="HGC5430911.1"
                     /translation="MGKEPEWKVDKQPAWLVAAIRRTIADLPHGYEEAAEILGLYKSD
                     DITPAKDQLHNRLRSGGDQIFPLEWAMVLQDASGTRHVTDAIARRSNGVFVPLVVIDD
                     IDNGDINQRLMESIEWIGKHSQYLRKATADGVIDQAEREQIEENSYQVMAKWQEHLTL
                     LFRVFCAPEKSNARECAAPGVVASIASGCGETNA"
     gene            complement(10124..10321)
                     /locus_tag="ACIYSB_003976"
     CDS             complement(10124..10321)
                     /locus_tag="ACIYSB_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGC5430912.1"
                     /translation="MRKSDVINYFGGVCKTAEALGIKHPSVSEWPEIIPEGRAYQLEK
                     ITNGKLKVDVSLYQKTNSAAA"
     gene            10417..11070
                     /locus_tag="ACIYSB_003977"
     CDS             10417..11070
                     /locus_tag="ACIYSB_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGC5430913.1"
                     /translation="MMNMSDRIRQRRKELNLTQQALADLTGVNRVTVTGWEKDDYQPN
                     GANLQALANALKCDPLWLVSGKGSPEPKINLKPEIFAVKKVPLISWVQAGSWTMTEPG
                     VRKEDAEEWVYTTALVSEMAFALRVRGDSMTNPLGSPSIPEGSIVIVEPDIIDTECIN
                     GKIVVAHINGGQEATLKKFVEDWPNRYLVPLNPNYKTIECGENCRIVGLVKQVIMDF"
     gene            11528..11890
                     /gene="yfdP"
                     /locus_tag="ACIYSB_003978"
     CDS             11528..11890
                     /gene="yfdP"
                     /locus_tag="ACIYSB_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGC5430914.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            11956..12780
                     /gene="yfdQ"
                     /locus_tag="ACIYSB_003979"
     CDS             11956..12780
                     /gene="yfdQ"
                     /locus_tag="ACIYSB_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HGC5430915.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISNIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAIRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEDMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            12908..13444
                     /gene="yfdR"
                     /locus_tag="ACIYSB_003980"
     CDS             12908..13444
                     /gene="yfdR"
                     /locus_tag="ACIYSB_003980"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGC5430916.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYELPPVMSTPVKYADLIMLATERRDLGLDDGSLWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            13435..14313
                     /locus_tag="ACIYSB_003981"
     CDS             13435..14313
                     /locus_tag="ACIYSB_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HGC5430917.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVVKFWDIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLIPFTKYCDNHFGRGNYITWLGIRADEPRRLKPKSGVRYLAE
                     LSDFDKSDVIRWWHKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRKTNKDVMYRGHLSLDGIARMYADSDYRNLYQAMVQARRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            14310..14498
                     /locus_tag="ACIYSB_003982"
     CDS             14310..14498
                     /locus_tag="ACIYSB_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430918.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRQHWCEGEVR"
     gene            14495..14686
                     /locus_tag="ACIYSB_003983"
     CDS             14495..14686
                     /locus_tag="ACIYSB_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430919.1"
                     /translation="MNGQISIVRPGACDDCEIRMIIRLARGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            14689..14985
                     /locus_tag="ACIYSB_003984"
                     /pseudo
     CDS             14689..14985
                     /locus_tag="ACIYSB_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            15232..15423
                     /locus_tag="ACIYSB_003985"
     CDS             15232..15423
                     /locus_tag="ACIYSB_003985"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGC5430920.1"
                     /translation="MPKKNQNVLISVNFDSDLVEPLICSARYTGSTFRRGDATIKPGN
                     GIEQATHWMPLPEPPQEVK"
     gene            15423..15995
                     /locus_tag="ACIYSB_003986"
     CDS             15423..15995
                     /locus_tag="ACIYSB_003986"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGC5430921.1"
                     /translation="MNNLMIDLETMGKNKDAPIVSIGAVFFTPETGDIGQEFYTVVSL
                     ESAMGQGATPDGDTILWWLKQSPEARAAICIDDTLSISDALSELNHFINRHAANTKYL
                     KVWGNGATFDNVILRGAYERAGQICPWAYWNDHDVRTIVTLGRSIGFDPKMDMPFDGE
                     RHNALADARHQAKYVSAIWQKLIPATSTEL"
     gene            16032..16304
                     /locus_tag="ACIYSB_003987"
     CDS             16032..16304
                     /locus_tag="ACIYSB_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HGC5430922.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRAEWERVRTHKQRLI"
     gene            complement(16338..16799)
                     /locus_tag="ACIYSB_003988"
     CDS             complement(16338..16799)
                     /locus_tag="ACIYSB_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430923.1"
                     /translation="MLGDCFPPDFKANFSRERGISPGDVLYLHCDFTTPPKVKYMVVV
                     CCEPLLVLLINSDINEFIKRNNDLMACQVEINREDHDFLKWDSFVNCIEAHAAFDLEI
                     IKEKIAIQYGDVLKGRITDHCMRQVRCAVEISKTMVKRHKKLILAALQHYE"
     gene            complement(16805..17386)
                     /locus_tag="ACIYSB_003989"
     CDS             complement(16805..17386)
                     /locus_tag="ACIYSB_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HGC5430924.1"
                     /translation="MRLHLPELDDVVWMKGVLMLNVRFDSEKALEAILYVASKAPIPD
                     IYHVGKILYYADRFHLESFGRLITGDHYNAMKDGPVASNTYDIIKIARGDGRYIPNGC
                     DVDSVRKAFSVSGMTIVPSREADEDFFSDSDLECIDKSIAMLGNMSFEAIRTMSHDAA
                     WEQADHNGEMSLESIVAQLKNSKLILDYLKNGY"
BASE COUNT         4767 a         4382 c         3870 g         4495 t
ORIGIN      
        1 gatttgaccc gcccctcccc tcaggctgtt gagaataatt atcacttaag ccgttcacgg
       61 gccgtcttcg ccttatgaca cggccagcac agactctgca gattgctgtc ggcatctgtg
      121 ccgccatgcg ctttaggaat gatgtggtca acggttttcg cctcacgtac cacaccagca
      181 cgcagacata actgacacag gcctttgtca cgttgcagca cacgcacgcg aataacatcc
      241 cacttagaac cataaccgcg ctgatgacgg gattgtcctg gcttgtattg cttccagccc
      301 tcgcttttgt ggctttcgca atagcctgac ggatcagtag tggtattgcg gcagccgcga
      361 acgcggcagg cttttggggt tcgtggtggc atgtaaaata tccataatga gagagtactt
      421 gtctctctca tataaaaatc aatctatgaa cacgacaatt ctagaatata tttttcctat
      481 ttttgataag tagcactttt tatttccacc agtcataata tattgactat aatcagtcct
      541 gagttgtttc cattcagcat gtatactttt atctttaatc ccctcttcac ataaaaccca
      601 tgctctcact gcattctgat atgctgattt aaggttttct ttcttgaaat atagctgtgc
      661 tgcatcatca actcggtgaa caacagcatt atactgctgt tcaactcttg ttctagctat
      721 tctgtattgt gtggaattcc aattatcggg cattgcctca aaggcatcct ccagctcaag
      781 taaagctcgt ttaaactcta actttacttt aaattgctct tgtctattcc atgtgtttaa
      841 cgccttgcgt gcataacaca acgcccataa agacgcgata gccgaaccac aagctccaat
      901 agcagaccac atattccaat ccatcaatat gcctccagtt aaagaagcaa tattaacatg
      961 gaatcatctc tatgaaatta caggtccttt tatttgttag cgtcgatatt ttttattgtc
     1021 gctttatcaa gattacactg ttccaacgct gacaacagac tcacattcaa ctccagactg
     1081 tcaccatagg tcagagtatc gggtatgtgc ggtactggcg tttccaaagt cagagtagct
     1141 ggaagcggaa ttggaggtac tgttacgtaa actgtccgcg tatttccgca accggtcagc
     1201 agcggcagca ggcacaggac gtgaagcaca atcatcatcc gcaacagcca ctttgatatc
     1261 ttcctgggtt ctctgtgact ccagtgcgat ctgctgtttt gcatgttgat tcgcctcctg
     1321 aatgatgttc gttattgcca tagtacgcag aacattcgcg gtgatagcct cagtagaatc
     1381 agctcgctgt tccgcagcat cagcacgctt ctgctcctcc agaaactttc catgatagtg
     1441 attcgctgac cagacaagac caccagcgac acaagcaata aacgttaaaa tgagcgccca
     1501 ataactcatc ttcataccag cagcgccgcc cgcgccttgt tgtatcggac cttacgatcc
     1561 tcaataccgt tcaaaccgcc gttaatgatg cgcgtaacac ggttaatatc ggctccgtag
     1621 atcatgcaac ctttagaggt gtagaaccat gcagctgagc gcgcagcctg tagttcctgt
     1681 tccagttgtt caggtgaagt caccagatct aacttcagcg ccgcgccaca gatgcgataa
     1741 ttatggaggc cagtgatttg aattaatcct ctaccacgat atttccagcc atcacctggt
     1801 gctttgttac ccagtcggtt gctatacacc agattggcaa tagcatcctg acgagctgca
     1861 tgtccggatg ttctgccaag ggcatcagcc tgctgctgtg tgatcctctt tccgaacgtc
     1921 gccaccagcg cagatggtgt gtagttaaaa ttttcaacta cggcgctaaa ccccatcgac
     1981 tcatggccta cctgagcgat aaacattgcc tgatccgctg gtgctgtaat gccgaattcc
     2041 ttcatcgccg catcaatgtg cggaaaccag cgcgcagcca gcccggcgct aataccagcc
     2101 gccttttgaa ataattgttg gttcattagt gcctcagatg atcaaccaga cgtgcaacgt
     2161 tgcctctgac ggccaccagc acggaaagaa aaatagtgtt cgccacgata atgggccatg
     2221 aggaatgagg ataaatccca cagagatagg ccaacggaac agcactgtat gtaacagtaa
     2281 tcagccaggc taaacgtgaa acccaaggac gatgccgcga atcaccacga cgataaaaca
     2341 tcagagtaat aacaacacaa gcacataaca gcgcatttat agttgctgtc gggtcattta
     2401 gctccacctg aacctccccg gcgcgttatg agcgccacca gcgagccgat atcctgatta
     2461 ttcaggaacg tcaggatttt aacggctaaa gcagagacga ttacggcacc aatagcatcc
     2521 agaggtttat cactgtatcc ggtcaagttc gccagcttgg agccaaccaa cccagagcaa
     2581 agaatcccgg caatatatga cacgataaaa tatgccagtc ggcgcgatgc actcagatct
     2641 gctgctgttg ctatgtagaa tacagcccct gcaaatgcgc caaatacaac gccgtaatca
     2701 gttccggtta acaacccata gatactagct cccgtcagga cacctccagc caacccggag
     2761 ccagaaatag gatcggacat aatcccccca tagatattac tgtgaatcct ctcagaaatt
     2821 aatgagggga atgaaaaata tattcacggt gctcatataa gtaatgatta aagataattc
     2881 ccatatcatg agaaagccac taagcagagg ctcaaaagat agatgcaatt acttactcat
     2941 catttcagaa atttcacatg aaagaccttc cagtatttta tccacagatt tatcgaattc
     3001 ccccccagca tccaaaatag tggctatagc ttcatttata tttttaggtc ttgagccatt
     3061 catgatacta aaataaatat cattagctgg cttcaattct ttttgcgcct ttttaatctg
     3121 aagcgccaca tccggaaaat aaataccagc aagaattgat gacaattcag aaattttaca
     3181 aaactctggc gtgttaagat ttcgctcgat ggttttatca agcgtttgtt ctatagatag
     3241 attacctcct acaagaagaa cccaatcact atgagtttgg aatactgatt ttttccaaag
     3301 aactaagttt ctgtataact cttcggcctt ctctattttt ttctttttat tttctttaac
     3361 ccattcattc ttttgtttcg aataatgaat taaataagtc attacattcg aaataacagt
     3421 accaacaaaa acacccaaag ccgctaaaag agcaacgttt tccctagtca attccataac
     3481 tccaccacaa aaaaaacccc accgaggtga ggttatcata gtaaatggta gttatacaaa
     3541 gcccattgtt gaaaacattt tatccatgtt ttttgaaaaa tgcaagcatc atgtcgctat
     3601 cttcggcgaa aatcatttat ttcgtcactt ttcttaattg cacctcagca tatgcttctt
     3661 cctgccagca ctttgtaacc agtttatcaa tgacatctgc atatcctttg taccactgat
     3721 aatccgtcaa gtctggtacc agcttctgga catgatgccg tgccagtgtg gtaggtaaac
     3781 ggctaaaccg gtttccattg caacgcccac aaatcttata aacaggcgtg ccatgaagcc
     3841 gggtcctttt ttcatccagg acaatacctt tacccttaca ccctctgcac gctgtgctga
     3901 cttctccctt accatgacaa tgctgacata gttccttcac ccactcttcc ttgataacag
     3961 actccccgct tctggagtgt ttcaccactt cgcgcaatac attatgaaat cccgtacctg
     4021 cacaatgctc acagcgagcc ttacttgccg cagacctgga ataatcagca aaggcaaaat
     4081 tcacaaggta aggaatgatc tgtagccggg tttcttcact caatttattc aatgtcgggt
     4141 tatccagtgc catcgcgtaa ttgagcagac cttcaatcgc aaactgagga tcctgaacac
     4201 caacttttgc caggaataag gcaaacccaa gcggtgcttt cgactgcacc atcccctgcg
     4261 cagccatcac atccgtaatc gttaaaccac cagagcctgt cgccggtgcg tcatcgctca
     4321 gttttggaga tttcggggag taatatttcg gtaaggcttc aaggttcatg ctcgttctcc
     4381 acttacgcca gtacgcctat tgccagcgca cgatcgataa aacgaaatat cagctccagc
     4441 tgggaaccat acttctcttc aaatgccacg gtatccgcat gcagctcgtc gtgatgcttt
     4501 ctgcacaaag gcaacacaaa gaggtcatgc gcttttgtac ccattccccc ctgaccgtgg
     4561 cctatcaggt ggtggggatc atcagcgggc tttccacaac atgcacacgg ctgtgtctta
     4621 acccagcgcg tgtacttttc attaacccag cggcgacgtt ttgggcgtaa cataaaagac
     4681 tccggcgact ccggatccac tttcagcgcc agcacctttt ttgccttatc ctggatgatg
     4741 ctggtggcag gaaccgaagg cacaaggtca ctttcccggg tgacagacgg cacaacaggc
     4801 tttggtaatc tcagtgcctt acgggctgca ctttccggta aggcatccgc caggtcatta
     4861 cgaatcagcc accagcacag ttccggcatt gtcacaacgt gactatcatc aaaaccgaga
     4921 tcccgacgca caacagacaa cacccagcgg gcacagttat ccgttgccat tgattccagc
     4981 cgttccgtga actgatcgcg cagctggtta tcacagtgcc agcacagacg gattgcgccc
     5041 ggagcgtgtc gcattgtggt catgttctcg ctgtgccagt cggaatgagg ccactggcag
     5101 cctttttcac gaagtaacca gctttcaaga cattccacgc caccagcacg acggatcact
     5161 gcctcattgc ggaacacggc ccgaacagca ggatcatccg ccagcggttg tgatgccgcg
     5221 ggaacggcac cactggcgaa agatgaataa cgttccggct caggctccag cagtacacgc
     5281 ccctgcataa acaggggcat cagctctgaa cctggtctga acaatacgat ccccatacgc
     5341 ggggctattt caggggtcag tagtgctctc acggtcacct caatgaacgg tatcgagcaa
     5401 ctttaacagc tcagggaatc gggattcgaa gaaatgcggc tgcgtctcgc gcggatttgc
     5461 gggactggtg atgttcttgc cgaacatgca gcctttcgcc gtcagcgacc agaatttttt
     5521 gatgttgtta atcgcggtac ggctgtatcg ttcgcgctgc tcgacgatcc ccagcttcgc
     5581 catctggtga tatgcctgat tagccgtaag gcggatacca tactgtttca gcagtgcact
     5641 cagcgacagt gtcgggcgac ttgagccatc aggcgcgcca gcaggagcat caatggcata
     5701 gcgcggtgcc agattcggta agccaacagc ctcctggagt ttctgacagg caccaagcac
     5761 tgaagagtta gacaggttta attcccggcg cataaagtcc agcagaatca caccagcctg
     5821 catcttgtca gcagcctgtc cggataattt ttccggtgcg ctggttacca tgtcgaaagt
     5881 acggatcacc ttcagatgga atgacgggct gatccacatt gcataggcat acaccagttc
     5941 tttgcagaca tacgtcccct ggttatttcc gccacgaata acgttaactg gctctatatt
     6001 gaccgagttg caaatctgca actcgcttat taaacgttca gtttgctcat tgcggagcca
     6061 gaatgcaggc ttatgcttat ccagagaacc ggcagccctg tgcagatcgt tcaggctgta
     6121 acgcccataa gcatcacgac gaacttcaat accatcaata accatcagat tattcatact
     6181 tcgtttctcc tcttaatcag gcggctgcac ccgccggttt ctcatactta ctgatagtga
     6241 tctcgacctt ccctttcggg ataaccggtc cccactccac cagcattctt ttcacctgtc
     6301 tgtcgtcttc ccacacaccc gcgtgggtca gggcgtcaaa cagcgccttg ttatagttat
     6361 ccagatcgcg gatccggtta tccggaggaa acaacacgat ctccactgaa gcaggtgccg
     6421 acgttggttt cggcagacga cgtaactgct caatgatggc ggcacacgcc gcgctctgga
     6481 attttcgtcc cgccgtgctt atcaggctct taccagcaaa tgcccctttg ttggggtgtc
     6541 gccagtacgt gttcacgctg ggcggaaaag gcagtattag cttcatgcca ccatctccct
     6601 gaccagtttt tccgcctgct ggcgaacctg cgccagaaat gcctcaccac atgcctcaag
     6661 ttcatcgcgc ccgatgtagc tgattgccgg tcccttccag gtcttgtcga aaacagcaat
     6721 agcaccagcg aagaaagcac ctgttggcac ctgcttttcg tctttcggga taaaccaggc
     6781 aggcagttca aaaccaatac gcccacgaat aaaagcaata tggtctgcat cttccggcca
     6841 ccacacttcg ctggtggcag ctttgatcag gaaaacatag cgcccgcctt tatcacgcat
     6901 ggcactggca tgtttcatga tgtaacgcat gccggtgatg tatttcccct catgctgact
     6961 ggcgcggctg tatgggggat taccaaaggc agcaccttta agctccgcaa gacgttctga
     7021 ccagtcatgc gccagcgcgt tgtcttccgc cgtgtaatac gcagcacatt tggcgttatc
     7081 accgtcagtg aacagatcca gaacaaacgg gccaaacatg gtgttaattc cccagaaaat
     7141 gctgtccggc gtgcgccact gatcgcccac ttccttcagt tcatgggctg gtttgttccg
     7201 cagttctacc agcgcctggc aatatttatt actcattaag cccccacgta aaaagcatcc
     7261 gcaatgtctc cggaagtaca gcccggatgg gcttcaatga atttctgaac ttcattcaaa
     7321 agactcatga tcaccccctg aatcctgccg ggatctggct gtagtccacg ttgtcgtaac
     7381 tggctttgaa gtacgggtcc tcacgtctgg ctgcagatac cgcaggaact tcccaggatt
     7441 cttcgaaatg acgatccgga ccaaagaacg tgacagcctg tttcacaaat tgtgtgccgc
     7501 tgttacccat cgcagatacc cagcccgcgt agcgtttcac accttccagc atggtttcgg
     7561 ggtttactcc ctcattcaaa cgggctttcc aggctttgaa ggctgcagat tttgaattgc
     7621 caccagcacg tttgggatat gccagccatg cctgctcaaa ctccggagag tattccggtc
     7681 ggtttgaacg aactcgcacg gactcatcaa ctgatgcacc aacagctatt ggttcattga
     7741 ctggttcttt gactggttca aaagagtgac tggttctggg tgaatctcct gcactacccc
     7801 ctggtgcaac tcctgcacta cctagtgaat ttgctgcacc agatagtgaa ttatttgcac
     7861 taccccctag tgaatctcct gcaccatcca gatgaaggag atagatatta cttgagttac
     7921 ctttttcacc tttccgggtg acttttttta ccagcccgga ctcacaaagg gccgcaatat
     7981 gattcatcac agaacgtttg ctaatctcgc actggtcagc aatatgctgg tagcttggcc
     8041 agcactcacc ctgatcgctg gcattatcag ccagcttgat cagaaccagt tttcgcaatg
     8101 gattacccac tcgaattttc atcgctttaa ccatcagctc catactcatg ctgcacctcc
     8161 gagatgcttc atgttttttc cggagcgaaa ggctataagc ggcatactga cgcggtaatt
     8221 acgaccaagc ggttcacaaa tcaccttctg acattcacgg tccaccaggc taacacgtag
     8281 aacatgccct gcaggcgtgg tgtaccactg accggggcga ggacaacgga aagtctgatt
     8341 ggtaaatcgt ttgaaaatat tccggatcat ttgcgccccc ttacctctga agagttcagc
     8401 gacgaatgaa taagacgggc aagaaatgcc gcatcgttaa ttcggtcata cagacttaca
     8461 gccagcggtg attcagcttt ttccagcatg ggataaagct gctgcaacca gacctgatga
     8521 attgatgaaa tgtaggaata gagaacgctg gcgttatgtg caacgtcgct cggtacagag
     8581 ggctttgaaa gctgtttctc catctggtta aaggcattga tgtatgcctc tttgaactgg
     8641 gcagcacgtt tacccgtgaa acccatagca agaaacgcaa agccatcgcg ggttatttga
     8701 tagcaaggta gtttgcggcc tgaagcgtcg gtataaccac ttaacacaaa attgtgttca
     8761 gcaaattcag cggagcattc aaggtttcga attctatcta aaacccgctc atgccgttta
     8821 gtaaagtagt tagcaactgc aagagatgta gtgacagcac gaccattgat gatcgtgatt
     8881 tcagggtgaa caaaggttgg gatcgtagcc ataatggcag cctcggtagc aagcttaaaa
     8941 aactcaccac ataagacgcc aatcatagag gtggtgagac gtacagagtt ggcgttaccg
     9001 gcggctaccc ttaccggcgc atcttgcgat gcccctgtac gccccaccat aatttgggcg
     9061 tgacaaaata atgcgtacaa aaaaaccgca tctacgcggt tgtacgcggt agccttctcc
     9121 aggacgccaa tcccggcacc cgctttataa ggtgcctgaa cagtgtaacg tcccagaatg
     9181 gcagaatcaa tgtgctggtg gtccttcaca ctcaacaaaa tcacgcctga atttccacaa
     9241 aggactaaag cactcatgcg ggtagtcttt gcgaagatag ataacgcgct gtgtttctgg
     9301 ctcccaacga ataacatgaa cataaagtcc tcttccgtca cgaaaccagc ggttaagttc
     9361 ctgcacaact cgccccccac agtcaggtaa agttctctgt ggttacttac agccaggtga
     9421 tttggtaatc tgcattcatg ccgtaacaac aggtgtgcag cgacactgac caccagctgt
     9481 tgcgacaaac ggttatttgc cgttaaactg ttcatgcgtt agtttctcca caaccagaag
     9541 caatcgacgc cacgacgccc ggagctgcac actcgcgggc gttactcttt tccggcgcac
     9601 aaaaaacacg aaataacagt gttaaatgct cctgccactt cgccattact tggtagctgt
     9661 tctcttcgat ttgctcacgc tcagcctggt caataactcc atcagcagtt gccttgcgta
     9721 agtactggga atgcttgcca atccattcta ttgactccat cagccgctga ttaatgtcac
     9781 cattgtcaat gtcatcaatg accaccagcg gcacaaacac cccattacta cgacgggcta
     9841 ttgcatctgt tacatgcctg gtaccactgg catcctgtaa aaccatggcc cactcaagtg
     9901 gaaaaatttg atccccaccg ctacgcagtc tgttatgcaa ttgatctttt gctggggtga
     9961 tatcatcaga tttatacaaa ccaagaattt ctgctgcttc ctcatagcca tgaggtaaat
    10021 cagcaatcgt tcttcgtatt gctgccacca gccatgctgg ttgtttatca actttccatt
    10081 caggttcttt acccacggtt aattcctcat ttctgtggtg tttttatgcc gcagcactgt
    10141 tagtcttttg atataaagac acgtcaactt tcagtttccc gttagtaatt ttttctaact
    10201 ggtacgctcg gccttcagga ataatctcag gccactctga aacagacgga tgcttaatac
    10261 ctagggcttc ggcggtttta caaactccgc cgaaataatt aatcacgtcg gatttccgca
    10321 tttctgtctc ccgttaaatt acgttaagca gaaatgtagg atatccaaca tgtcaatgtc
    10381 aagaatccta catgggcatg tggtaggatt gcctacatga tgaacatgag tgatcgtatt
    10441 cgccaaaggc gaaaagaact gaacctgaca caacaagcac tggctgattt gactggtgtg
    10501 aaccgtgtca cggttactgg atgggaaaag gacgactacc aaccaaatgg agccaacctt
    10561 caagccctag ccaacgcact taaatgcgat cctctgtggc ttgttagcgg aaaaggctcg
    10621 cctgaaccaa agataaatct aaaacctgaa atattcgcag ttaaaaaagt ccccctcatc
    10681 tcgtgggttc aggcgggttc atggacaatg acggagcctg gtgtcaggaa agaagatgct
    10741 gaagagtggg tttatactac cgcccttgta tcagaaatgg catttgcact acgggtccgt
    10801 ggtgattcaa tgaccaatcc cctcggctca ccatcgatac cagaaggttc tatcgttatc
    10861 gtagagccag atattattga tacagagtgt attaacggaa aaatcgttgt tgcccatatc
    10921 aatggtgggc aagaagcgac actcaaaaaa tttgttgagg actggccgaa caggtatctc
    10981 gtcccactaa atcctaacta taaaactatt gaatgcggtg agaactgcag aatagttggt
    11041 cttgtcaaac aagtaataat ggatttttga cacatcttcc tcactatcgc aaaaccgggg
    11101 catccccggt ttttttatga gcctatcttt ttatgtagga taaccaacac aaactcttga
    11161 cactcacatg ttggatatcc tacatttgtt tttagagttg tggtgaatgc gcaggctgat
    11221 gcgcgaaaga cattgcagct attgcggaaa agagctgttc ggcggggcaa ttaaacgccc
    11281 gtgagagtct gaaataaccg caagccggag atcagcaccg gtcaccacaa cagccactgc
    11341 tttggcggta ccagtttgta cacttgcttc cggctggtac cgctcttttt acaaaacaga
    11401 gaagagcatc accggacgac gggctcataa cccaatccat ccgggcggct gccaccgcag
    11461 gtgttcttct ctgttttgtg gagaaaccaa ccgaccttgc agggtcgata tgatgaggag
    11521 cagcaaaatg gctagcgaac gcagtactga tgtgcaggca tttatcgggg agctggacgg
    11581 cggcgtattt gaaaccaaaa tcggcgctgt tctcagtgaa gtcgcttccg gtgtgatgaa
    11641 cacgaaaacc aaaggtaagg tctcgctcaa cctggaaatc gaaccgtttg atgagaaccg
    11701 tgtgaaaatc aaacacaaac tctcatatgt tcgcccgact aaccgcggga aaatttccga
    11761 agaagacacc accgaaacgc cgatgtatgt caatcgcggt ggtcgcctga ctattctgca
    11821 ggaagaccag ggacaattac tgactcttgc cggtgaacct gacggaaaac tccgcgcagc
    11881 aggtcattaa tatcgttctt aattaactga ttatttatct catcactgaa tatcttaata
    11941 tagtgaggac ttattatgtc tcagaactta gacgcaaccg caattaatca aatccatgcc
    12001 cttatttctg ctcagggtgt taatgaaatt atcagtaata ttggtgccga tgctgtggca
    12061 ttgcctgaga atttccgcat tcatgatctg gaaaaattta atttaaatcg cttccgtttc
    12121 cgtggtgcgc tttccactgc cagcatcgat gactttaccc gttattctaa agatcttgca
    12181 gatgaaggca cccgctgctt tatcgatgct gataatatgc gtgccgtcag tgtgcttaac
    12241 ctgggtacta ttgatgaacc aggtcacgca gataacaccg ccactctcaa actgaaaaag
    12301 acagcaccgt tctctgccct gttgtctgtt aacggcgagc gtaactccca gaaatcactg
    12361 gcagaatgga ttgaagactg ggccgactac cttgtgggct ttgatgctaa tggtgacgcc
    12421 attcaggcaa caaaagcggc tgcggcaatc cgtaaaatca cgattgaagc aaaccagacc
    12481 gctgattttg aagataatga cttcagcggc aaacgctccc tgatggaatc tgtcgaagcg
    12541 aagaccaaag acattatgcc agtggcattt gaatttaaat gcgttccgtt tgaaggtctg
    12601 aaagaacgtc cgtttaaatt acgcctcagc attatcactg gcgatcgtcc tgtactggtt
    12661 ctgcgcatta ttcagctgga agcggtgcag gaagatatgg ctaacgaatt tcgtgatctg
    12721 cttgttgaga aattcaaaga cagcaaagta gaaaccttta ttggtacttt caccgcctga
    12781 tttcattact gcaaatgccc ctgcgggggc atttatggaa acgtaattaa ctcaataatc
    12841 accggatggt gagggcttcc ttttacccaa attcagcgcg gtgcagcgca tatacgtgga
    12901 gaacaaaatg tcatttatta aaactttttc cgggaagcat ttttattatg acaagataaa
    12961 taaagacgac atcgtgatta acgatatcgc ggtttccctt tcaaatatct gccgctttgc
    13021 cggtcatctt tctcacttct acagcgtcgc ccaacatgcg gtgctttgca gccagctggt
    13081 gccgcaggaa tttgcttttg aagcgttaat gcatgatgca acagaagcgt attgccagga
    13141 catccccgca ccactgaaac gccttcttcc tgactataaa cggatggaag aaaaaataga
    13201 tgcagtaatc cgtgagaaat acgagttgcc cccggttatg agcacgcctg tgaaatatgc
    13261 cgatctaatc atgctggcaa ccgaacgccg tgatctcggg cttgatgatg gctctttatg
    13321 gcctgtactg gaaggtatcc cggcaacaga gatgttcaaa gttattccac tggcaccagg
    13381 ccatgcctac gggatgttta tggaacgttt taacgagtta tcggaattac gcaaatgtgc
    13441 ataactcatg tagttagttt ttctggcggg agaacatccg catatcttgt tcacctgatg
    13501 gaagaacaaa gaaaggctgg caataacgtc tgctacatct ttatggatac cggttgcgaa
    13561 catccgctga cataccgctt tatccgggag gttgtgaagt tctgggacat accactaact
    13621 gtgttacagg tcgatataaa tcctgagctt gggcagccaa atggttatac agaatgggag
    13681 ccaaaggata ttcagacacg aatgccggtg cttaaaccgt ttatggacat ggttaaaaag
    13741 tacggcacgc catacatcgg cggcgcgttc tgtactgaca ggctaaaact catccctttc
    13801 acgaaatact gcgataacca tttcgggcga ggtaattaca tcacatggct gggtattcgt
    13861 gcagacgaac cccgtaggct gaaaccgaaa tcgggcgtcc ggtatcttgc cgagctgtca
    13921 gattttgata agtcggatgt tatccggtgg tggcataaac aaccttttga tttgcaaatc
    13981 ccagagcacc tcgggaactg tgttttctgc atcaaaaagt ccacgcaaaa gctggggctt
    14041 gcatgtaaag acgaacctgg tctgatgcga gtttttaatg agctggttac aggtaaacac
    14101 gtccgggatg gtcaccgaaa gacaaataaa gacgttatgt accgtggtca tctgagcctt
    14161 gacgggattg ccagaatgta tgccgacagc gactacagaa atttgtatca ggcgatggtg
    14221 caagccaggc gattcgatac cggctcgtgt tcagagtcat gtgaaatctg gggtgatcaa
    14281 ttggaattga aattcgaaga ggtggtggca tgacaatcgt aaaaacccat accggcattg
    14341 tgatcaccaa agatggcccg caggtaaaaa aattgcacca aacagagcgg atgtgggtcg
    14401 tcggcaaaaa cgagttttac cacaaagaaa ctggacgccg ccactttgca gaaaatacgc
    14461 gccgccgcca acattggtgt gaaggggaag tgagatgaac ggacaaatct caattgttcg
    14521 accgggagca tgtgacgatt gcgagatacg aatgattatt cgtctggcga gggggaaaac
    14581 aataactgct ctcattactc ctgaaaatct cgcattagca ttaacaggaa agtcagacct
    14641 gccagtagag ctaaagctgc gaaatattga gattaaagtg aaatagttat gaatactctt
    14701 accaaagaat ggttacagaa cacgattacc agcattgagt cagcacggga tgaaataccg
    14761 ttcggactcg atgaagatca aaacaacatg cttaccgcat taaaaattgc actggcatca
    14821 ctggcagcag tatcggatga acgagcagcc tatgaattat ttatggagaa gcgtttcggg
    14881 gaatctgtag atcgccgtag ggcaaaaaat ggcgatagag aatacatggc atgggatatg
    14941 gcgcttggct ggattatctg gtgtcaccgc gccgccatgc ttcagggtag ccaacctgta
    15001 agccaaactt acaacttgcc agaattaatc gaaggcatgg aagtttccat tgatgtaagc
    15061 acttgtgatg ctgatttagg taatcgctat ttcggcaccg tcaccgaggc gttagaactt
    15121 gatacagcca agaatggtta catcctcctg gttcaggacg cagagccaaa cttcgatgta
    15181 aatggcaact ctccggtaac tccggatggt tggataagct gtagtgagct aatgccgaaa
    15241 aagaatcaga acgtacttat ttcggtgaat ttcgatagtg atctggttga accgctaata
    15301 tgctccgcac gctataccgg aagcaccttt cggcgcggag atgcaacgat taagccgggt
    15361 aatggtattg agcaagcaac tcactggatg ccgctaccgg aaccgccgca ggaggtgaag
    15421 tgatgaacaa cttaatgatc gaccttgaga cgatggggaa aaataaggat gcaccgatcg
    15481 tttccattgg cgcggtgttc ttcactccag aaaccggaga catcggacaa gaattctata
    15541 cggttgttag cctggaaagt gctatggggc aaggagctac acctgacggc gataccatcc
    15601 tgtggtggtt gaaacaaagc cctgaagcac gagctgcaat ctgtattgat gatactttgt
    15661 cgatcagcga tgctctctca gaactaaatc atttcattaa ccggcatgca gccaatacga
    15721 aatatttaaa agtctggggt aacggggcca ccttcgacaa cgtaatttta cgtggagctt
    15781 atgagcgagc aggacaaatt tgcccgtggg catactggaa tgaccacgat gtacgcacga
    15841 tcgttacgct tgggcgttcc atcggattcg accccaaaat ggacatgcct ttcgatggcg
    15901 aacggcacaa cgccctggcc gatgcccgtc atcaggcaaa atatgtttcc gctatctggc
    15961 agaaattaat tcctgccacc agcacagaat tatgattttc ccgggtgcag ccggttttga
    16021 tggagaaaat tatgaacacc ttgtttttac tgatggctga attcaatacc cctaacattg
    16081 aactctcagc agttagccaa aagtactttg gcatgagtcc agccacggca gaagcaaaag
    16141 caaacgcttg taagttgccc gttccaacat atcgcatcgg cacatcacaa aaagcaaaac
    16201 gttgcatcaa tattcaggat cttgcggaat acatagacaa aaggcgagaa gaagggcgtg
    16261 ctgagtggga aagggtcaga acccataaac aaaggctcat ttaaatagaa tatgaataaa
    16321 cccatccaaa gatgggttta ttcataatgt tgaagagcag cgagtatcag ttttttatgc
    16381 cgtttaacca tagttttaga tatctcaact gcacatcgaa cttgtctcat acaatgatct
    16441 gtaatgcgac cttttagcac gtcaccatat tggatggcta ttttctcttt aataatttca
    16501 agatcgaaag cagcatgagc ctcaatgcaa ttaacaaatg agtcccattt aagaaaatca
    16561 tggtcctctc tattaatttc gacctgacaa gccataagat cattgtttct cttaataaat
    16621 tcatttatat ctgaatttat tagaagaact aaaagaggtt cacagcaaac aaccaccata
    16681 tattttactt taggtggggt cgtaaaatca caatgaagat acaatacatc accgggtgat
    16741 atacctcttt cacgactaaa attagcctta aaatcaggag ggaaacaatc acccagcata
    16801 agtatcaata tccgttcttc agataatcca gtattaattt gctatttttt agctgtgcaa
    16861 ctatcgattc caaagacatc tcaccattgt gatctgcctg ttcccatgcc gcgtcatggc
    16921 tcatggttct gatagcttca aaggacatgt ttccaagcat cgcgatagac ttatcaatac
    16981 actctaaatc tgagtcacta aaaaagtctt catcagcttc acggctcggc acaatcgtca
    17041 tacctgatac agaaaatgct tttcgcacag aatcgacatc acaaccatta ggaatgtaac
    17101 gtccatctcc acgagcaatt tttataatat cgtatgtgtt gcttgctaca ggcccatcct
    17161 tcatagcgtt atagtgatcg cccgttatga ggcgtccaaa actttcaagg tgaaacctgt
    17221 cagcataata aagaattttt ccgacatgat agatatctgg gatcggtgct ttagaggcga
    17281 cgtacagaat ggcctctaaa gccttttctg aatcaaacct tacatttagc atcaatacac
    17341 ccttcatcca aacaacatcg tcaagctctg gcaaatgcaa ccgcatccgc cttctgcaat
    17401 cttaaagtct tcacctacgc taggtcaatt gaagtgatga gcaaatgatg ctttctaaga
    17461 agaacaggtt cccaataggc ccccacaaag tgtataacta cttgtttttc aaga
//
LOCUS       DBBGLQ010000090        16854 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.090, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000090 DBBGLQ010000000
VERSION     DBBGLQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.090"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..790
                     /locus_tag="ACIYSB_003990"
     CDS             <1..790
                     /locus_tag="ACIYSB_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGC5430925.1"
                     /translation="TVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISNQGKLG
                     EHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFLHMIGS
                     HPNPCDRLNSWPNYYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAMLYFSD
                     HGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKINARNFAGIFQWLTGI
                     RTENITPFNPLTDEDNEPVMVFNGEKNVPADSLKPQPLILPDHR"
     gene            complement(1114..2379)
                     /locus_tag="ACIYSB_003991"
     CDS             complement(1114..2379)
                     /locus_tag="ACIYSB_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5430926.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARKLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWATELSLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFCGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDANRGKFISPFEYAKINNPLKQ"
     gene            complement(2566..2650)
                     /locus_tag="ACIYSB_003992"
     tRNA            complement(2566..2650)
                     /locus_tag="ACIYSB_003992"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2614..2616),aa:Leu,seq:caa)
     gene            2846..3865
                     /gene="ahr"
                     /locus_tag="ACIYSB_003993"
     CDS             2846..3865
                     /gene="ahr"
                     /locus_tag="ACIYSB_003993"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGC5430927.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            3993..5495
                     /gene="yjgR"
                     /locus_tag="ACIYSB_003994"
     CDS             3993..5495
                     /gene="yjgR"
                     /locus_tag="ACIYSB_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGC5430928.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRTFTPKDQKAV
                     KAAAQTMRVNPAFDTEKAIQKLGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5656..6738)
                     /gene="lptG"
                     /locus_tag="ACIYSB_003995"
     CDS             complement(5656..6738)
                     /gene="lptG"
                     /locus_tag="ACIYSB_003995"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGC5430929.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6738..7838)
                     /gene="lptF"
                     /locus_tag="ACIYSB_003996"
     CDS             complement(6738..7838)
                     /gene="lptF"
                     /locus_tag="ACIYSB_003996"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGC5430930.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7901..7951)
                     /gene="ytgA"
                     /locus_tag="ACIYSB_003997"
     CDS             complement(7901..7951)
                     /gene="ytgA"
                     /locus_tag="ACIYSB_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGC5430931.1"
                     /translation="MSIIILATISTTQENK"
     gene            8105..9616
                     /gene="pepA"
                     /locus_tag="ACIYSB_003998"
     CDS             8105..9616
                     /gene="pepA"
                     /locus_tag="ACIYSB_003998"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGC5430932.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9970..10413
                     /gene="holC"
                     /locus_tag="ACIYSB_003999"
     CDS             9970..10413
                     /gene="holC"
                     /locus_tag="ACIYSB_003999"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGC5430933.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10413..13268
                     /gene="valS"
                     /locus_tag="ACIYSB_004000"
     CDS             10413..13268
                     /gene="valS"
                     /locus_tag="ACIYSB_004000"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGC5430934.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(13322..14518)
                     /gene="yjgN"
                     /locus_tag="ACIYSB_004001"
     CDS             complement(13322..14518)
                     /gene="yjgN"
                     /locus_tag="ACIYSB_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HGC5430935.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLFFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRHQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLPANSSSSVIISMVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VNLDTTYCIKYAILAFLALFPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     SSLPFL"
     gene            14711..15214
                     /gene="yjgM"
                     /locus_tag="ACIYSB_004002"
     CDS             14711..15214
                     /gene="yjgM"
                     /locus_tag="ACIYSB_004002"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGC5430936.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(15260..15676)
                     /gene="rraB"
                     /locus_tag="ACIYSB_004003"
     CDS             complement(15260..15676)
                     /gene="rraB"
                     /locus_tag="ACIYSB_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGC5430937.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            15838..16842
                     /gene="argF"
                     /locus_tag="ACIYSB_004004"
     CDS             15838..16842
                     /gene="argF"
                     /locus_tag="ACIYSB_004004"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGC5430938.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT         4334 a         4241 c         4315 g         3964 t
ORIGIN      
        1 taccgtacct tccctgagcc ggacactgat ttatgactat gagcagaacc ctgactccgg
       61 caacaacgtg gtggcgctgg cagcaaaagc aggatacagc acatggtgga tatccaatca
      121 gggaaaactg ggagagcatg acacacgcat ctctgttatt gcttctgatg cggagcatac
      181 cgttttcctc aagaaaggca gcttcgcttc ccgtaaaacg gatgacatgt tgttgttaca
      241 ggaaacagaa cgtgcgctgg cggataaatc ctcgccgaag gtgattttcc tgcacatgat
      301 tggctctcat ccgaatccgt gtgaccgact taactcctgg ccgaattatt acctggagca
      361 gtatccccga aagattgcct gttacctcgc cagcatcagt aaactggata actttctcgg
      421 tcagcttgat ggtatccttc gccggcattc ccgtcacttt gcaatgcttt acttttctga
      481 ccatgggctg tcggtcagcg acagtgctaa tcctgttcat catgatggtc atgtgcaggg
      541 gggctacagc gttcccctga ttattaccgc cagtgacatc acgtctcatc agtccgtcag
      601 caggaaaatc aatgcccgta atttcgcagg catttttcag tggctgaccg gtattcgtac
      661 cgaaaatata acgccattca atccgctgac agacgaagat aatgaacccg ttatggtttt
      721 taacggagag aaaaatgtgc cggcagacag tctgaaaccg cagccactta ttcttccgga
      781 ccacaggtaa tcatatatgg caaactgtaa tacagtctct tgtactgaaa aggattctgt
      841 atagaataga acctgtattc atatcagcac aagggaccac caaatcccgg caacattcct
      901 tacgtcagtt atcccgcatc accctgtggg tgcgggattt tttatgatgt ttctttttat
      961 tgaatatata taaataacga ataactgcgg attctgataa tccacctgca gggagcaatc
     1021 cggtaaacat ttaatacatc agttaatatc gttttgcctt tttaacaaag aaaatattca
     1081 tggagtaagt tcccgggcat ttgcccggga tgattactgt tttaatggat tattaatctt
     1141 tgcatattca aatggactga taaattttcc tctgtttgca tccagaaaat cggcccacca
     1201 ttgtagcatc aagcggcgtt cttccagatg ctctgcttta tggatatacg cggccctcac
     1261 tgaatttcgc tccatgtggc tcatctgacg ttcaacagca tcacgagacc acaaacctga
     1321 ttcgaccaat gaactacagg ccattgttcg aaagccatga ccacaaacct ctacttttgt
     1381 atcatacccc atgacccgta acgcactatt taccgtattc tcactcatgg gtttgtgcga
     1441 atcgtgatca ccaataaata tcaagtcatg ggccccacaa aactgtttta tctgctttaa
     1501 aattgcaaga gcttgcgttg aaagaggcac tagatgcgtt gtacgcattt ttgagcctct
     1561 atgggaatgt ttcactccag gaataggctc ccgctccggt gggatagtcc atatagacgc
     1621 ttcgaaatcg atctctgacc aacgagcaaa acgcagctca ctggaccgaa taaagatcag
     1681 caaagagagt tctgtcgccc atcgggttag cggcctacca gtatagctat ctatttttgt
     1741 aagcaactca gggatgcgct ttaattcaag cgcgggacga tgttgtcgat tacaggaagc
     1801 aaccgcccca gccatctctt gtgccgggtt ataatcaatt aacccacttt gcactgcata
     1861 gcgcatgatg gctgtagtgc gctgctgaag acgagcggcc acttcaagac gtccagacat
     1921 ttctacggcc ttaatgggtg ctaataaatc tcgagttttt aactcagcga tattacgttc
     1981 accaagcgct gcaaaaagat tatcttccag actttttagc acacgatggg cgtgatcttc
     2041 agaccacttt ttattggtgc catgccactc aatcgcgact tctttaaagg ttcgtgcttt
     2101 actctgttca accttatcat ttttcttttt gtctcccgga tcgacgccat tcgcaagcag
     2161 cttacgcgcc tcgtcacgac gtactctggc atccgctagt gtgatttcag gataaacccc
     2221 aagtgccagc attttttgct ttccctcata acggtactgc aaacgccagt acttagaacc
     2281 atttggatgg acaagcagat gcataccgcc accatcagtc agcttatatt gcttatccgt
     2341 tggcttggct gctctgactt tgatatctgt taatgccatg cgtattccct ccctgttggt
     2401 acaagcatta tcgaaccgga aataccatca tctgtaccaa catatgttgg tagatgtacg
     2461 ttgatatcgg ttgaccttga gagagttaat atagcgggaa aggtagataa acactggatt
     2521 tcaggcataa aaatagacct caactgaggt ctatttacat acttttggtg ccgaaggccg
     2581 gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct accgatttcg
     2641 ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt cgcacgccac
     2701 catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc attacactca
     2761 gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct acacttccca
     2821 aacaacacca gagaaggacc caaaaatgtc gatgataaaa agctatgccg caaaagaagc
     2881 gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac aagatgttga
     2941 agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg ataacgaatg
     3001 gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg tggtggcact
     3061 cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga ttggctggac
     3121 ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca actgcgagca
     3181 aggtgcggtg ccgacgatta tgaatcgcgg tggctttgcc gagaagttgc gtgcggactg
     3241 gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc cgctgttgtg
     3301 cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta ccagccgcgt
     3361 tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc acgcaatggg
     3421 atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac tggcgatggg
     3481 tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt cggggcagtt
     3541 tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt ttgaggcgct
     3601 gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt ctgttccggc
     3661 ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca cgccttatga
     3721 gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa ccgaactgtt
     3781 cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag ctcgctaccg
     3841 agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg gcacttaaat
     3901 ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca cagccgttgc
     3961 ctatactcca ggcaggaact ggaggaaatc tcatgagcga acccctgtta attgcccgca
     4021 cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg ctgattactg
     4081 gcgcaacggg gacgggtaaa accgttacgc tgcaaaaact ggcagagtca ttgtcggaaa
     4141 tcggcgtgcc ggtgtttatg gctgatgtga aaggcgatct gaccggtatc gcgcaggcag
     4201 gaacggcgtc ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat gactggcaac
     4261 cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccat ccggtgcggg
     4321 cgacggtttc ggatctgggg ccgctgttgc tggcgcggct gttgaatctc aacgatgtgc
     4381 aatctggcgt gctgaatatc atcttccgca ttgctgacga tcaggggctg ttactgctcg
     4441 actttaaaga tttgcgggcg attacccaat acatcggcga taacgccaaa tctttccaga
     4501 atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcggg ctgttgtcgc
     4561 tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc aaagactgga
     4621 tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag aagctttatc
     4681 agatgccgaa actgtatgcc gccagcctgt tgtggatgct ctcggagttg tatgaacaat
     4741 tgccggaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac gaggcacatc
     4801 tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg ataaggctta
     4861 ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa tccgtccgat attccggata
     4921 acgtgctcgg ccagctcggt aatcgcgttc aacacgcttt gcggactttt acgcccaaag
     4981 atcagaaagc ggtaaaagct gcggcgcaaa ccatgcgggt caatccggca tttgataccg
     5041 aaaaggcgat tcagaaactg ggcaccggcg aggcgttgat ctcttttctg gatgcgaaag
     5101 gaagcccttc tgtggtggaa cgggcgatgg tgattgcgcc ttgttcgcgg atgggaccgg
     5161 tgacggaaga tgagcgtaat ggcttgatta atcactctcc ggtgtatggc aaatatgagg
     5221 atgaggtgga ccgggaatcc gcctatgaga tgttgcaaaa aggctttcag gccagtaccg
     5281 agcagcaaaa taatcccccc gcgaaaggga aagaggtagc ggtggatgac ggcattcttg
     5341 gtggattgaa ggatattttg tttggcacta ctggaccacg cggcgggaag aaagatggtg
     5401 tggtgcagac aatggcaaaa agcgccgccc gccaggtgac gaatcagatt gtgcgcggga
     5461 tgttggggag tttgctgggg gggagaagaa ggtaagccag gtgcgatttg tctttgcctg
     5521 atgcgacgct ggcgcgcctt atcatgccca caccactgca atatattgaa ttttcatgat
     5581 ttttcaggca tgaacgaagc acactttgct aacaataata aagggagctt tcgctccctt
     5641 tattcgttca tcaggttacg attttctcat taacagccac aggctgatta agaagaagct
     5701 ggcacttggc aaaagtgcgc cgatgatcgg cgggatgccg taaaccaacg tcagcgggcc
     5761 gaagatctgg tccagtacgt agaagacaaa accgaagcta ataccagtga ccacacgcac
     5821 gcccatcggt acgctacgca gtgggccaaa gatgaacgac agcgccatca gcatcatcac
     5881 cgccacggat agcggctgga agattttgct ccacatgttg agctgataac gtccggcatc
     5941 ctgaccgctc gacttcagat acttcacata gttgtgcaaa ccgctgatag agagtgcatc
     6001 cgggtccagc gccaccacgc ccagtttgtc tggcgtgagg ttggttttcc aggtgccgct
     6061 caccgtctgc gaaccggtga tctgtttcgg attggtcaaa tcagattcat caacctgcga
     6121 cagacgccag actttatgtt cagggtcaaa cttcgcggta gcggcatagc gtacggattg
     6181 cagacgacga ttctcgttaa aggcatagat actgatgcca cctaactctt cgtcaccttt
     6241 aacccgctca atgtagacga agttgttgcc atctttcgcc cataaacctt gctgggtaga
     6301 gagcaacgag ccgccgtaca tcgcctgcgc acggtagtta cgcgccatct gctcgccctg
     6361 cggcgcgacc cattcaccaa tcgccatcgt cagcaagacc agcggaatgg cggttttcat
     6421 caccgacagc gccacctgca tacgagtaaa accagaagcc tgcatgacta ccagttcgct
     6481 gcgctgcgcc agcatcccaa gaccaagcaa cgccccaagc agagccgcca tcgggaagaa
     6541 gatttgcaca tctttcggca cgctcagcaa ggtatacatt cctgcgccta acgcgtcgta
     6601 actcccctgc ccggcttttt tcagctgatc gacaaacttg ataatgcccg acagcgacac
     6661 cagcatgaac agcgtcatca tgatggtggt gaaaatagtt ttaccgatat agcggtcaag
     6721 tacgccaaaa ggttgcatca caccgctcct ttacgcgaaa aactggcgcg caggcggcgg
     6781 accggcacgg tgtcccaaag gttgagaaca atcgccaaag ccagataaat caggttaacg
     6841 gtccacatcc acagcgtcgg gtccagctta cctttaccgc cgttcgattt cagggaggtc
     6901 tggatcagga agaacaacag atacagcagc atggctggca gcatcgatag tacgcgcccc
     6961 tgacgcgggt taaccacgct cagcggtacg accataagtg ccatcataaa cacggtgaac
     7021 accaacgtga tacgccagtt cagttctgcg cgagcacgat cggtgtcagt gttccacaat
     7081 gtgcgcatgt ccatctggtc ggtatcgttc gggtcgagcg ccaccgcctg gtgaccaatg
     7141 atcgcctgat aatcctggaa atccgtaatg cggaaatcac gtaacagtgc agtgccttcg
     7201 aagcgcgttc cctggttgag agtgacgacc tgggagccgt cgcgcagctg ggttaaatgt
     7261 ccggaatcgg ccaccaccac agaaggacgt gcattacctt ttggtcgaat ttgcgcgagg
     7321 aacacatctt tgaaatcgct gccgtcaacg ctttcgatga acagcaccga actgccatta
     7381 gtcgcttgct ggaattgccc ttgcgccagc gccgccatgc cagggttcgc tttcgcttct
     7441 gctaacactt catcctgatg acgcgatgac cacggtcccg cccacatcac gttaaccgcc
     7501 gcgacgattg ccgtgaatac cgcaaggatc attgccgctt tcaccagaac cgctttgctc
     7561 aggccgcagg catgcattac cgtaatttca ctttcggtat acagtttgcc cagcgtcatc
     7621 agcagcccga ggaacaggct taatggcagg ataagctgcg ccatttccgg cacgcccaac
     7681 ccgagaaggg agagcaccag attcgccgga atatcgccgt caaccgctgc gccgaggatc
     7741 ctcactaact tttgacagaa gaagatcaaa agcaagatga agagtatcgc cagctggctt
     7801 ttgagcgtct cccgcaccag atatcttatg attatcactt taaatacgcc cgtaaaaact
     7861 cgtcttttgc aggattttag cttgtttcat ggcttaaacg tcatttattc tcttgagtcg
     7921 tcgaaatcgt cgctaagata attatactca acggattcac ctctcagatt ttgttctgac
     7981 gtgccaatgc cgtaataacg ttaagattaa cacgaagtca tcgcaacagc ggacatgagt
     8041 tacgaaagct tgcaattcta tctgtagcca ccgccgttgt ctttaagatt caggagcgta
     8101 gtgcatggag tttagtgtaa aaagcggtag cccggagaaa cagcggagtg cctgcatcgt
     8161 cgtgggcgtc ttcgaaccac gtcgcctttc tccgattgca gaacagctcg ataaaatcag
     8221 cgatgggtac atcagcgccc tgctacgtcg gggcgaactg gaaggaaaac cggggcagac
     8281 attgttgctg caccatgttc cgaatgtact ttccgagcga attctcctta ttggttgcgg
     8341 caaagaacgt gagctggatg agcgtcagta caagcaggtt attcagaaaa ccattaatac
     8401 gctgaatgat actggctcaa tggaagcggt ctgctttctg actgaactgc acgttaaagg
     8461 ccgtaacaac tactggaaag tgcgtcaggc tgtcgagacg gcaaaagaga cgctctacag
     8521 tttcgatcag ctgaaaacga acaagagcga accgcgtcgt ccgctgcgta aaatggtgtt
     8581 caacgtgccg acccgccgtg aactgaccag cggtgagcgc gcgatccagc acggtctggc
     8641 gattgccgcc gggattaaag cagcaaaaga tctcggcaat atgccgccaa atatctgtaa
     8701 cgccgcttac ctcgcttcac aagcgcgcca gctggctgac agctacagca agaatgtcat
     8761 cacccgcgtt atcggcgaac agcagatgaa agagctgggg atgcattcct atctggcggt
     8821 cggtcagggt tcgcagaacg aatcgctgat gtcggtgatt gagtacaaag gcaacgcgtc
     8881 ggaagatgca cgcccaatcg tgctggtggg taaaggttta accttcgact ccggcggtat
     8941 ctccatcaag ccttcagaag gcatggatga gatgaagtac gatatgtgcg gtgcggcagc
     9001 ggtttacggc gtgatgcgga tggtcgcgga gctacaactg ccgattaacg ttatcggcgt
     9061 gttggcaggc tgcgaaaaca tgcctggcgg acgagcctat cgtccgggcg atgtgttaac
     9121 caccatgtcc ggtcaaaccg ttgaagtgct gaatactgac gctgaaggcc gcctggtact
     9181 gtgcgacgtg ttaacttacg ttgagcgttt tgagccggaa gcggtgattg acgtggcgac
     9241 gctgaccggt gcctgcgtga tcgcgctggg tcatcatatt actggtctga tggcgaacca
     9301 caatccgctg gcccatgaac tgattgccgc gtctgaacaa tccggtgacc gcgcatggcg
     9361 cttaccgctg ggtgacgagt atcaggaaca actggagtcc aattttgccg atatggcgaa
     9421 cattggcggt cgtcctggtg gggcgattac cgcaggttgc ttcctgtcac gctttacccg
     9481 taagtacaac tgggcgcacc tggatattgc aggaaccgcc tggcgttctg gtaaagcaaa
     9541 aggcgcaacc ggtcgtccgg tagcgttgct ggcacagttc ctgttgaacc gcgctgggtt
     9601 taacggcgaa gagtaattgc gtcaggcaag gctgttattg ccggatgcgg cgtgaacgcc
     9661 ttatccgacc tacacagcac tgaactcgta ggcctgataa gacgcgcaag cgtcgcatca
     9721 ggcaatgctg ttattgtcgg atgcggcatg aacgccttct ccgacctaca cagcaccgaa
     9781 ctcgtaggcc tgataagacg cggcagcgtc gcatcaggca atgctgttat tgtcggatgc
     9841 ggcgtgagcg ccttatccga cctacacaca gcactgaact cgtaggcctg ataagacaca
     9901 acagcgtcgc atcaggcgct gcggtgtata cctgatgcgt atttaaatcc accacaagaa
     9961 gccccatata tgaaaaacgc gacgttctac cttctggaca atgacaccac cgtcgatggc
    10021 ttaagcgccg ttgagcaact ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag
    10081 cgcgtgctca tcgcctgtga agatgaaaag caggcttacc ggctggatga agccctgtgg
    10141 gcgcgtccgg cagaaagctt tgttccgcat aatttagcgg gagaaggacc gcgtggcggt
    10201 gcgccggtgg agatcgcctg gccgcaaaaa cgcagcagca gcccgcgcga tatattgatt
    10261 agtctgcgaa caagctttgc agattttgcc accgctttca cagaagtggt agacttcgtt
    10321 ccttatgaag attctctgaa acaactggcg cgcgaacgct ataaagccta ccgcgtggct
    10381 ggtttcaacc tgaatacggc aacctggaaa taatggaaaa gacatataac ccacaagata
    10441 tcgaacagcc gctttacgag cactgggaaa agcagggcta ctttaagcct aatggcgatg
    10501 aaagccagga aagtttctgc atcatgatcc cgccgccgaa cgtcaccggc agtttgcata
    10561 tgggtcacgc cttccagcaa accatcatgg ataccatgat ccgctatcag cgcatgcagg
    10621 gcaaaaacac cctgtggcag gtcggtactg accacgccgg gatcgctacc cagatggtcg
    10681 ttgagcgcaa gattgccgca gaagaaggta aaacccgtca cgactacggc cgcgaagctt
    10741 tcatcgacaa aatctgggaa tggaaagcgg aatccggcgg cacgattacc cgtcagatgc
    10801 gccgtctcgg caactccgtc gactgggagc gtgaacgctt cacgatggac gaaggcctgt
    10861 ccaatgcggt gaaagaagtt ttcgttcgtc tgtataaaga agacctgatt taccgtggca
    10921 aacgcctggt aaactgggat ccgaaactgc gcaccgctat ctctgacctg gaagtggaaa
    10981 accgcgaatc gaaaggttcg atgtggcaca tccgctatcc gctggctgac ggcgcgaaaa
    11041 ccgcagacgg taaagattat ctggtggttg cgactacccg tccggaaacc ctgttgggcg
    11101 ataccggcgt ggccgttaac ccggaagatc cgcgttacaa agacctgatt ggcaaatatg
    11161 tcattctacc gctggttaac cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg
    11221 aaaaaggcac cggctgcgtg aaaatcactc cggcgcacga ctttaacgac tatgaagtgg
    11281 gtaaacgtca cgcgctgccg atgatcaaca tcctgacctt tgacggcgat atccgtgaaa
    11341 gcgcccaggt gttcgatacc aaaggtaacg aatctgacgt ttattccagc gaaatcccgg
    11401 cagagttcca gaaactcgag cgttttgctg cacgtaaagc agtcgttgct gcggttgatg
    11461 cactcggcct gctggaagaa attaaaccgc acgacctgac cgttccttac ggcgaccgtg
    11521 gcggcgtggt tatcgaaccg atgctgaccg accagtggta cgtgcgtgcc gatgtactgg
    11581 cgaaaccggc agttgaagcg gttgagaacg gcgacattca gttcgtaccg aagcagtacg
    11641 aaaacatgta cttctcctgg atgcgcgata ttcaggactg gtgtatctct cgtcagctgt
    11701 ggtggggtca ccgtatcccg gcatggtatg acgaagcggg taacgtttac gttggccgca
    11761 acgaagacga agtgcgcaag gaaaataacc tcggtgccga cgttgccctg cgtcaggacg
    11821 aagacgttct agacacctgg ttctcttctg cgctgtggac cttctctacc cttggctggc
    11881 cggaaaatac cgacgccctg cgtcagttcc acccaaccag cgtgatggta tctggtttcg
    11941 acatcatctt cttctggatt gcccgcatga tcatgatgac catgcacttc atcaaagatg
    12001 aaaatggcaa accgcaggtg ccgttccaca ctgtttacat gaccggtctg attcgtgacg
    12061 acgaaggcca gaagatgtcc aaatccaagg gtaacgttat cgacccgctg gatatggttg
    12121 acggtatttc gctgccagaa ctgctggaaa aacgtaccgg caatatgatg cagccgcagc
    12181 tggcggacaa aatccgtaag cgcaccgaga aacagttccc gaacggcatt gagccgcacg
    12241 gcaccgatgc cctgcgcttc accctggcgg cgctggcgtc taccggtcgt gacatcaact
    12301 gggatatgaa gcgcctggaa ggttaccgta acttctgtaa caagctgtgg aacgccagcc
    12361 gctttgtgct gatgaacacg gaaggtcagg attgcggctt caacggtggt gaaatgacgc
    12421 tgtcgctggc ggaccgctgg attctggcag agttcaacca gaccatcaaa gcgtaccgcg
    12481 aagcgctgga cagcttccgc ttcgatatcg ctgcaggcat tctgtatgag ttcacctgga
    12541 accagttctg cgactggtat ctggagctga ccaagccggt aatgaacggt ggaaccgaag
    12601 ccgaactgcg cggtactcgc catacgctgg tgactgtact ggaaggtctg ctgcgcctcg
    12661 cgcatccgat cattccgttc atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg
    12721 gtatcactgc cgacaccatc atgctgcagc cgttcccgca gtacgatgca tctcaggttg
    12781 atgaagcggc gctggccgac accgagtggc tgaaacaggc gatcgttgcg gtacgtaaca
    12841 tccgtgcaga aatgaacatc gcgccgggca aaccgctgga gctgctgctg cgtggttgca
    12901 gcgcggatgc agaacgtcgc gtaaatgaaa accgtggctt cctgcaaacg ctggcgcgtc
    12961 tggaaagtat caccgtgctg cctgccgatg acaaaggtcc ggtttccgtt acaaagatca
    13021 tcgacggtgc agagctgctg atcccgatgg ctggcctcat caacaaagaa gatgagctgg
    13081 cgcgtctggc gaaagaagtg gcgaagattg aaggtgaaat cagccgtatc gagaacaaac
    13141 tggcgaatga aggctttgtc gcccgcgcac cggaagcggt catcgcgaaa gagcgtgaga
    13201 agctggaagg ctatgcggaa gcgaaagcga agctgattga acagcaggct gttatcgccg
    13261 cgctgtaatc aacaccgtga tgaaaacgaa ggccggagca tgctccggcc ttttttatct
    13321 cttacagaaa tggtaaagaa gacataactc cccgtgaaat actggctaag aagcctttat
    13381 ctggttgttc ttctttattg attaaaggaa ggtcatccaa atcgcccagc aaatacgtat
    13441 tttttgcctg ccagtcaatc atccatattt tcagcagtgg gtaagccaga ccacccgtaa
    13501 tcccggatat caccaccaac gcacacatgc gataaagcat accgtggtac gttaaagttg
    13561 agcgaaaacg aatacgccca tcattcagtg acaggttgct cataaaatga tttcgcaacg
    13621 acaccgttaa ataactggtg ctaacagcaa tcccaaaata atagattaac tgcgcgatta
    13681 tcattttacg ctgcatttcc ataaattgct gtaaattctc aatgtcatca tttgcatata
    13741 cagaactatc atacgcattt aatatttgat cgaagataat ataaccagca acagcgagaa
    13801 aaggaaataa agctaaaaat gcgagaatgg catattttat acaatacgta gtatctaaat
    13861 tcaccttgaa acgatgtata ccgaaactgg tattgctcca gagaaaactc attaccagac
    13921 tatataaagt accattaaaa ataccaatgg aaacaatacc aactattgcc atcagaacca
    13981 tggatataat aacactactt gaactattgg caggtagcat ctttgtcgag ataaagaaaa
    14041 cagtccccat cccaatggcc attaaaatcg gcaagaaaaa ggtcacccac cagaaccctt
    14101 tcatagagca attaaaacta aatcttacgc cgttgagact ggtcattaag gcctgatgac
    14161 gcagtccttt tgctgccata aatataagca aaaccaacag taacaaagtc aaaacacaac
    14221 caacaagcgg catatctgct gacactgtca ttaagattgc gaaatagaaa aaaacaaaaa
    14281 aaagacaact aacaaaaaca ttcccaccgg taattccata agaaaatcgt tgtccgttaa
    14341 cttccatatt agcataaaga taacgcttac atttcattaa tgcccatggt aaatagatac
    14401 ctagcgttat aatcgttaac aacacattca ccacgcaaat aagaaaatat ctctcacctg
    14461 taccatgaaa aacaaacgaa tgggacggaa catccatttc attaataact tgagccataa
    14521 aagttccctg aattaaaaaa tccattgata taacttatat atacagacag taaaatgttc
    14581 tgccacgtta caatactctt atcagtaata ggctggcaaa aactttatta ctttataagt
    14641 cgcttgcgta cgtaagaaga gagtggtatt aaacaaatgt ataattactt tatttttatt
    14701 gggttaaact atgaataaca ttgcgccgca atcacctgta atgcgtcgcc tgacgctgca
    14761 ggataatcct gctatcgccc gcgtcattcg tcaggtatcc gccgaatacg gtcttaccgc
    14821 tgataaaggc tacaccgtcg ccgatccgaa tcttgacgag ctgtatcaag tgtatagtca
    14881 gcctggccat gcatattggg tcgttgagta cgagggtgaa gtggtcggcg gcggtgggat
    14941 tgcgccatta gcaggtagtg aatcggacat ttgcgaactg caaaagatgt attttcttcc
    15001 ggctattcgc ggtaaagggc tggcgaaaaa actggcctta atggcgatgg agcaggcacg
    15061 agagatgggc ttcaaacgct gttatctgga aacgaccgct tttttaaagg aagccattgc
    15121 actttatgag catttgggct ttgagcatat cgactatgcg cttggctgca cgggccatgt
    15181 cgattgtgaa gtgcggatgc tacgcgaact ctaaaaaaat gcccgctagt gtgtgctatc
    15241 gggcatttta atcaattaat tagtggcgaa ccccgtcatc gtcttcgtcg acaaaatctt
    15301 catcgtcgcc gtcttcgccg ttaggatctt caaagtaagt gccccaaccg tcgtactcaa
    15361 cgtcaaattt ctctgccagc gtcatcagtt gttcaacctg ggcatcgatc agatcggcat
    15421 tcaatgcgca ctcgctgagg atgtcgcagc aaatcacgat gtcaccatct tcaacttcca
    15481 gctcttctgg atcggtcact tcgtaaccga gtttaaacgc ttcaactgct gctttttcca
    15541 gtgtttccag atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg tccggatcgc
    15601 tgccatcttc cagtaattct tcaataatca aacgtgtttc ttcacgctgt tcttccagtt
    15661 gttccgggtt tgccatggct cattcctctt aatgtcctgc cgatactctt attgtcacac
    15721 accactgaca ttgcctccac ctttgtgaca aagatttatg ctttagactt gcaaatgaat
    15781 aatcatccat atagattgaa ttttaattca ttgaggcgat agccacagga gggatctatg
    15841 tccgggtttt atcataagca tttcctgaaa ttactcgatt tcacgccagc tgaactcaac
    15901 agcctgttgc agttagccgc gaagctgaaa gccgataaga aaagcggtaa agaagaagcc
    15961 aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag actcgacccg tacccgatgc
    16021 tctttcgaag ttgccgcata tgaccagggt gctcgcgtta cttatctcgg cccaagcggc
    16081 agccagattg gtcataaaga gtcgattaaa gacactgccc gcgtgcttgg tcgcatgtat
    16141 gacggtattc agtatcgcgg ctatggtcag gagattgttg aaacactggc ggaatacgct
    16201 ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc ccacgcagct gctggcggat
    16261 ctcctcacca tgcaggagca tttacctggc aaagcgttca acgaaatgac gctggtctat
    16321 gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg aagctgcggc gcttaccggt
    16381 ctggatttgc gtctggtcgc gccacaggca tgctggccgg aagctgcgct ggttacggaa
    16441 tgccgcgctc tggcacagca aaatggcggg aatattacgc taacggaaga tgtagccaag
    16501 ggagtagaag gtgctgactt tatctatacc gatgtgtggg tgtcgatggg ggaagcaaaa
    16561 gagaaatggg cagaacggat tgcattactg cgtgattatc aggtgaacag caagatgatg
    16621 cagttgaccg gtaacccgga ggtcaaattc ctccactgcc tgcccgcttt tcatgacgac
    16681 cagacgacgc ttggtaagaa aatggcggaa gagtttggcc tacatggcgg aatggaagtg
    16741 actgatgagg tcttcgaatc tgccgccagc attgtttttg atcaggcgga aaaccgcatg
    16801 cataccatca aagcggtgat ggtcgcgacg ctcagtaaat aattgatatt gcta
//
LOCUS       DBBGLQ010000091        16814 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.091, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000091 DBBGLQ010000000
VERSION     DBBGLQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.091"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACIYSB_004005"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACIYSB_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGC5430939.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACIYSB_004006"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACIYSB_004006"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGC5430940.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENTSKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACIYSB_004007"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACIYSB_004007"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGC5430941.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACIYSB_004008"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACIYSB_004008"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGC5430942.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEELFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACIYSB_004009"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACIYSB_004009"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGC5430943.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ACIYSB_004010"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ACIYSB_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGC5430944.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11195
                     /gene="ftsK"
                     /locus_tag="ACIYSB_004011"
     CDS             7245..11195
                     /gene="ftsK"
                     /locus_tag="ACIYSB_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGC5430945.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVPPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVE
                     ARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEV
                     IPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMP
                     HLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVV
                     TDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGD
                     SMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRP
                     SVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRV
                     HGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQF
                     VTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11354..11965
                     /gene="lolA"
                     /locus_tag="ACIYSB_004012"
     CDS             11354..11965
                     /gene="lolA"
                     /locus_tag="ACIYSB_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGC5430946.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            11976..13319
                     /gene="rarA"
                     /locus_tag="ACIYSB_004013"
                     /gene_synonym="mgsA"
     CDS             11976..13319
                     /gene="rarA"
                     /locus_tag="ACIYSB_004013"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGC5430947.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAISELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13410..14702
                     /gene="serS"
                     /locus_tag="ACIYSB_004014"
     CDS             13410..14702
                     /gene="serS"
                     /locus_tag="ACIYSB_004014"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGC5430948.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            14907..15128
                     /locus_tag="ACIYSB_004015"
     CDS             14907..15128
                     /locus_tag="ACIYSB_004015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430949.1"
                     /translation="MLFSLRTSFKATLVNDDGTPRITAEVRNVTPDDIGKVAELVRDV
                     FGTPSGTCRSDSAARVDRQDVNAGWLTRL"
     gene            complement(15279..15419)
                     /locus_tag="ACIYSB_004016"
     CDS             complement(15279..15419)
                     /locus_tag="ACIYSB_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGC5430950.1"
                     /translation="MPQKTIIVGILCLTITLVIWMVHDSLCEFSFDIWGARFAAFLQC
                     KP"
BASE COUNT         4240 a         4225 c         4402 g         3947 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 tattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tggccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagctttt ccaggccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaaaagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 agattgctcc ggataagtga actgaacatc ccgtaacgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggaa aggtgacctc cggtttttga tccgttaagt cagagatacg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcagcaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaatagcgcc cggcagtacg
     3001 ggtgattgct gcgccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg gaggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgcaat gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcgaccaac
     3841 caacaccgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gctcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccgcctca ccacgttgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcgcca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca caccggtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag attgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 cagatagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagcctgtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca ggcgcttaat gaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatccggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcctaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc ggacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca cctgcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc
     8461 cgtattatgc acctgcagct gaacaacctg cgcaacagcc gtattatgcc cctgcgccag
     8521 aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccact tttgctccac
     8581 agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag ccgttgtacc
     8641 aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga
     8701 caaaacccgc gcgtccgccg ctttactact ttgaagaagt tgaagagaag cgagcccgtg
     8761 aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa gaaccagaac
     8821 cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta gaagccgctg
     8881 ccgctgtttc cccgctggca tctggcgtga aaaaagcgac actggcgacg ggggctgccg
     8941 caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt cctcaggtca
     9001 aagaggggat tggtccgcag ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg
     9061 aactggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa aaagcccgtg
     9121 aagcccagcg caatcagtac gattctggcg atcagtacaa cgatgatgaa atcgatgcga
     9181 tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc tatggcgaac
     9241 agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca gaggctgaac
     9301 tggctcgtca gtttgcgcaa actcaacaac aacgttattc cggcgaacaa ccggctgggg
     9361 cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg ctggatgatg
     9421 gtccacacga accgttgttt acgccaattg ttgaacctgt acagcagccg caacaaccgg
     9481 ttgcaccgca gcagcaatat cagcagccgc aacaaccagt tccgccgcag ccgcagtatc
     9541 agcagccaca acagccggtt gcgccgcagc cacaatatca gcagccgcaa caaccggttg
     9601 cgccgcagca acagtatcag cagccacaac agccagttgc gccacaaccg caggataccc
     9661 tgcttcatcc gctgttgatg cgtaatggcg acagccgtcc gttgcataaa ccgacgacgc
     9721 cgctgccttc tctggatttg ctgacaccgc cgccgagcga agtggagccg gtagatacct
     9781 ttgcgcttga acaaatggct cgcctggtgg aagcgcgtct ggctgatttc cgtattaaag
     9841 ccgatgtcgt caattactct ccggggccgg ttatcactcg ctttgaattg aacctggcac
     9901 cgggcgtaaa agcggcgcgc atttctaact tgtcacggga ccttgcccgt tcactttcga
     9961 cggtggcggt gcgtgtcgtt gaagttattc ctggcaaacc ctatgtaggt ctggagttac
    10021 cgaataaaaa acgacaaacc gtttatctgc gcgaagtttt ggataacgcc aaattccgcg
    10081 ataatccgtc gccattaacc gtggtgctgg gtaaagatat cgccggtgag ccggtggttg
    10141 ccgatctggc gaaaatgccg cacttgttgg ttgcggggac taccggttcc ggtaaatctg
    10201 tcggtgtgaa cgcgatgatc ctgagcatgc tttataaagc acagccagaa gatgtgcgtt
    10261 tcatcatgat cgacccgaaa atgctggagc tttcggttta tgaaggcatt ccgcatctgt
    10321 taacggaagt cgttactgat atgaaagatg ccgccaacgc gctgcgctgg tgtgttaacg
    10381 agatggagcg tcggtataaa ctgatgtctg cgctgggtgt gcgtaatctg gcgggttata
    10441 acgaaaaaat tgctgaagcc gatcgcatga tgcgtccgat tccagacccg tactggaagc
    10501 cgggtgacag tatggatgcc cagcatccgg tgctgaaaaa agaaccatac attgtggtgt
    10561 tggttgacga atttgccgac ctgatgatga cggtaggtaa aaaagtggaa gagctgatag
    10621 cacgtctggc gcaaaaagcc cgtgccgcgg gtatccacct cgtactggca actcagcgtc
    10681 catcggttga tgttattact ggtctgatta aagcgaatat tccgacccgt atcgccttta
    10741 ccgtatccag taagattgac tcacgtacca ttcttgatca ggctggcgcg gaatcactgc
    10801 tgggtatggg ggatatgctc tactctgggc cgaactccac gttgccggta cgtgtccatg
    10861 gtgcttttgt tcgcgatcag gaagttcatg ccgtggtgca ggactggaaa gcgcgtggtc
    10921 gcccacagta tgttgatggc atcacctccg acagcgaaag cgaaggtggt gcgggtggtt
    10981 tcgatggcgc tgaagaactg gatccgttgt tcgatcaggc ggtgcagttt gtcactgaaa
    11041 aacgcaaagc gtcaatttct ggcgtacagc gtcagttccg cattggttat aaccgtgcag
    11101 cgcgtattat cgaacagatg gaagcgcagg ggattgtcag cgaacagggg cacaacggta
    11161 atcgtgaagt gctggcccca ccgccgtttg actaactaat gcatcgtatg ccggataagg
    11221 cgcagtagcg tcgcatccgg cactctatca actgaaaatt cagtattttc ttctttcctc
    11281 aagctgatta ttagcctgga atagagagta gagggaactc ccgatcggga gtgacgtaat
    11341 ttgaggaata atgatgaaaa aaattgccat cacctgtgca ttactctcaa gcttagtagc
    11401 aagcagcgtt tgggctgatg ccgcaagcga tctgaaaagc cgcctggata aagtcagcag
    11461 cttccacgcc agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg
    11521 tcagggcgat ctgtgggtga aacgtccaaa cttattcaac tggcatatga cacaacctga
    11581 tgaaagcatt ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca
    11641 agctacggca acctggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg
    11701 caaccagtcc agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgtcct
    11761 gacgccgaaa gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg
    11821 cacaatccat cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcaactgaa
    11881 atcccagcaa aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt
    11941 cacggtagat gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt
    12001 cggataatac ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata
    12061 tcggccagca acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc
    12121 atttacattc tatgatcctc tgggggccgc cgggtaccgg caaaacaact ctcgctgaag
    12181 tgattgcccg ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga
    12241 aagagattcg cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta
    12301 ttctttttgt tgacgaagtt caccgtttca acaaaagcca gcaggatgca tttctgccac
    12361 atattgaaga cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc
    12421 ttaattcggc actgctttcc cgtgcccgtg tctatctgtt gaaatccctg agtacagagg
    12481 atattgagca agtactaact caggcgatgg aagacaaaac ccgtggctat ggtggtcagg
    12541 atattgttct gccagatgaa acacgacgcg ccatttctga actggtgaat ggcgacgcgc
    12601 gccgggcgtt aaatacgctg gaaatgatgg cggatatggc cgaagtcgat gatagcggta
    12661 agcgggtcct gaagcctgaa ttactgaccg aaatcgccgg tgaacgtagc gcccgctttg
    12721 ataacaaagg cgatcgcttt tacgatctga tttccgcact gcataagtcg gtacgtggta
    12781 gcgcacccga tgcggcgctg tactggtatg cgcgaattat taccgctggt ggcgatccgt
    12841 tatatgtcgc gcgtcgctgt ctggcgattg cgtctgaaga cgtcggtaat gccgatccac
    12901 gggcgatgca ggtggcaatt gcggcctggg attgctttac tcgcgttggc ccggcggaag
    12961 gtgaacgcgc cattgctcag gcgattgttt acctggcctg cgcgccaaaa agcaacgctg
    13021 tctacactgc gtttaaagcc gcgctggccg atgctcgcga acgcccggat tatgacgtgc
    13081 cggttcattt gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat
    13141 atcgttacgc tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg
    13201 aaatagcaca aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggcg
    13261 aaaagctcgc ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat
    13321 gttatcgttg cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc
    13381 ccatttaatt cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc
    13441 cagacgcagt cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg
    13501 gcgctcttga agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc
    13561 gtaactcccg atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt
    13621 tacgtctgga agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg
    13681 ctttacaggc tgaaattcgc gatatcgcgc tgaccatccc taacctgcct gcagatgaag
    13741 tgccggtagg taaagacgaa aatgacaacg ttgaagtcag ccgctggggt accccgcgtg
    13801 agtttgactt tgaagttcgt gaccacgtga cgctgggtga aatgcactct ggcctcgact
    13861 ttgcagctgc agttaagctg actggttccc gctttgtggt aatgaaaggg cagattgctc
    13921 gcatgcaccg cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca
    13981 gtgagaacta tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcaactgc
    14041 cgaaatttgc tggcgatctg ttccatactc gtccgctgga agaagaagca gacaccagta
    14101 actatgcgct gatcccaacg gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca
    14161 tcgatgaaga tgatctgcca attaagatga ccgcccacac cccatgcttc cgttctgaag
    14221 ccggttcata tggtcgtgac acccgtggtc tgatccgtat gcaccagttc gacaaagttg
    14281 aaatggtgca gatcgtgcgc ccagaagact caatggcggc gctggaagag atgactggtc
    14341 atgcagaaaa agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg
    14401 gcgacatggg ctttggcgct tgcaaaactt acgacctgga agtatggatc ccggcacaga
    14461 acacctaccg tgagatctct tcctgctcca acgtttggga tttccaggca cgtcgtatgc
    14521 aggcacgttg ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt
    14581 ctggtctggc tgttggtcgt acgctggttg cagtaatgga aaactatcag caggctgatg
    14641 gtcgtattga agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct
    14701 aatacccaat ttttctgaat ctaaaaagcg cctgcgggcg cttttgcatg gtacttaact
    14761 atatgaaaat aaatggattt tattttcatg tgtccagata atgtccatct ttgaagaaaa
    14821 aagtccacaa tactgacgtt gtgggctttt ctttttcctg atggtgtcgt gccgtcagtg
    14881 caacgaacca aagggagggg atgttgatgt tgttttccct tcgcaccagc tttaaagcca
    14941 ctcttgttaa cgatgatggt acgccacgga tcactgccga ggtgaggaat gtgacacctg
    15001 atgatatcgg gaaggtggcg gaacttgtca gggatgtttt tggtactcct tccggtacct
    15061 gccgttcaga ctctgcggcc agggttgaca ggcaggatgt taacgccggg tggcttacgc
    15121 gcctctgaat gatgatgccc tcactggtga gggtgacaaa aaagccctct caatggcggg
    15181 caaatctggt tttttgtttt gtctctacag ggaaagggcg ccatatcggc ccaccccaac
    15241 aaccggatag cggggaattt ctcccccgct gcggtttctt acggtttaca ctgcaagaac
    15301 gccgcaaaac gcgctcccca gatatcaaag ctgaattcac ataacgaatc gtggaccatc
    15361 cagatgacca gggttattgt caggcataaa atgccaacaa taatcgtttt ttgcggtata
    15421 gcgcttgctc cttttacaaa gaggcgctaa ccttccactt gtcacggtag gaagggtcag
    15481 gcctcggtta aacacagatg ttttccgggg cctttccaca tccggccttc aggttttccc
    15541 tccggccatc agccgaaagg cacccgtttc tttttgttgc ggcaatattt caggtgtcag
    15601 ttggggtggg ctgtatcttg tctgatgcat aaaaaaatgc ccgcgctttg gcgggcaaaa
    15661 gacaagactt ttcgtttgta tatcatggat aatggacacc cttcctgttg tcaggtggca
    15721 ggcacccgtg gttaatctac tgtttttgtg cttttgtctc aaccctgaag ttttcatgga
    15781 aaatcagggt tgcttcatat gtgccaggaa ggtgcggcta tgataactgc caaaaaaagc
    15841 ccgctttccg gcgggcaaaa cagcaatgtg agttgtctgt cattgatact ggacagcctt
    15901 cctgttgtca ggtggaaggt acccgtggtt aatctactgt ctttgtgctt ttgtctcaac
    15961 cctgaagttt tcatggaaaa tcagggttgc ttcatgtgaa ccaggaaggt gaggcgatgg
    16021 taaccgccaa aaaaatgccc gctttccggc gggcaaaaca aggaagtacg gatcatgtat
    16081 tattgattac gaggcattcc accatgccca cgtttaattt agctagtttt cattttattc
    16141 acaatatagt tacttatcat atttatgaac aaaactgtaa tttcatctat aggtataagt
    16201 gatttgcatt aacatgtttc ttctgtattg ctgttggttt tccgatgctg atatttatga
    16261 tacattcaag ggcaaatgag gttgttccac agaaacgatg ttgagatatt tctgctccag
    16321 aaaggacaag ctaaaaaaat aaagtgatat tatgatatgt cagggatgtt caggcatgtc
    16381 agtgtcttat taataaactc gttgctgctg taataacacc aaaaaaatca aagcataatc
    16441 tgcatgttgt tcctgatgac ccaataacca caaaagaata aaaaaatgcc cgctttccgg
    16501 cgggcaaaac agcaatgtga gttgtctgtc attgatactg gacacccttc ctgttgtcag
    16561 gtggaaggca cccgtggtta atctactgtc tttgtgcttt tggctcaacc ctgaagtttt
    16621 catggaaaat cagggctgct ttgtgtgcgc cagggaaggc gcctcgatgg caggcgcaaa
    16681 aaaaatgccc gtgcttcggc gggcaaaata aggaagtaca ggttacatgt tattgataaa
    16741 gtagcattgc tctgtccgtc tttaatctaa cgagttttga gttttttcac aatattgtta
    16801 cctgtcatgt tata
//
LOCUS       DBBGLQ010000092        16612 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.092, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000092 DBBGLQ010000000
VERSION     DBBGLQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.092"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(148..684)
                     /gene="yghD"
                     /locus_tag="ACIYSB_004017"
     CDS             complement(148..684)
                     /gene="yghD"
                     /locus_tag="ACIYSB_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HGC5430951.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETILQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIADRGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(686..1864)
                     /gene="gspL"
                     /locus_tag="ACIYSB_004018"
     CDS             complement(686..1864)
                     /gene="gspL"
                     /locus_tag="ACIYSB_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HGC5430952.1"
                     /translation="MSSILEIFFPLCASDTVRWQRRTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMACDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1861..2838)
                     /gene="gspK"
                     /locus_tag="ACIYSB_004019"
     CDS             complement(1861..2838)
                     /gene="gspK"
                     /locus_tag="ACIYSB_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HGC5430953.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2841..3440)
                     /gene="gspJ"
                     /locus_tag="ACIYSB_004020"
     CDS             complement(2841..3440)
                     /gene="gspJ"
                     /locus_tag="ACIYSB_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HGC5430954.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(3437..3808)
                     /gene="gspI"
                     /locus_tag="ACIYSB_004021"
     CDS             complement(3437..3808)
                     /gene="gspI"
                     /locus_tag="ACIYSB_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HGC5430955.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTRGQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(3805..4368)
                     /gene="gspH"
                     /locus_tag="ACIYSB_004022"
     CDS             complement(3805..4368)
                     /gene="gspH"
                     /locus_tag="ACIYSB_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HGC5430956.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASSGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(4372..4827)
                     /gene="gspG"
                     /locus_tag="ACIYSB_004023"
     CDS             complement(4372..4827)
                     /gene="gspG"
                     /locus_tag="ACIYSB_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HGC5430957.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4844..6067)
                     /gene="gspF"
                     /locus_tag="ACIYSB_004024"
     CDS             complement(4844..6067)
                     /gene="gspF"
                     /locus_tag="ACIYSB_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HGC5430958.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPVSTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVIGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6067..7560)
                     /gene="gspE"
                     /locus_tag="ACIYSB_004025"
     CDS             complement(6067..7560)
                     /gene="gspE"
                     /locus_tag="ACIYSB_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HGC5430959.1"
                     /translation="MVPVAQETTVNTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDGAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7560..9620)
                     /gene="gspD"
                     /locus_tag="ACIYSB_004026"
     CDS             complement(7560..9620)
                     /gene="gspD"
                     /locus_tag="ACIYSB_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HGC5430960.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENEVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9650..10609)
                     /gene="gspC"
                     /locus_tag="ACIYSB_004027"
     CDS             complement(9650..10609)
                     /gene="gspC"
                     /locus_tag="ACIYSB_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGC5430961.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKMAHSLWRYISFSAEYTAVSQPVNKPSRVDAKTFDKNDVQLISQQNWF
                     GKYQPVAAQVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAVSDEAKQAVAEPAVSVPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAAKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(10627..11037)
                     /gene="gspS2"
                     /locus_tag="ACIYSB_004028"
     CDS             complement(10627..11037)
                     /gene="gspS2"
                     /locus_tag="ACIYSB_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HGC5430962.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11103..11912)
                     /gene="pppA"
                     /locus_tag="ACIYSB_004029"
     CDS             complement(11103..11912)
                     /gene="pppA"
                     /locus_tag="ACIYSB_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HGC5430963.1"
                     /translation="MLFDVFQQYPTAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMSSAQSKISLALPRSHCPHCQQTIRIRDNIPLFSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12110..>16612)
                     /gene="sslE"
                     /locus_tag="ACIYSB_004030"
     CDS             complement(12110..>16612)
                     /gene="sslE"
                     /locus_tag="ACIYSB_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HGC5430964.1"
                     /translation="LAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEPTPEPTP
                     DPEPTPEPIPDPEPTPEPEPEPVPTKTGYLTLGGSQRVTGATCNGESSDGFTFKPGED
                     VTCVAGNTTIATFNTQSEAARSLRAVEKVSFSLEDAQELAGSDDKKSNAVSLVTSSNS
                     CPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKAPSTHTS
                     PVVPVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGRLVDSQGYGVAGVNYYTNS
                     GRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQLIHR
                     YSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLGEGEQVVNLPNEFIEQF
                     NTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYKSVSKFH
                     VFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKNELAYIT
                     EAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPNGYSWNGGVN
                     KDGQCTLNSDPDDMKNFMENVLRYLSDDKWKPDAKASMTVGTNLDTVYFKRHGQVTGN
                     SAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYAIPLRADTSK
                     PKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMALNKSVVN
                     NDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTGEVKWKYQVENKPDDKPKL
                     EVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKEKIFAAFPGLKECTNPAYHYEVN
                     CLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRLYQHELYFRTNG
                     RKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNCYANDAYAGG
                     TKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNIKVD
                     VEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIKSNANVPVTV
                     TVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIKGNSSTNESA
                     SFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTTPKKNLNASNYTGGLEQFA
                     NDLDTFASSMNDFYGRDSEDGKHRMFTYKNLPGHKHRFTNDVQISIGDAHSGYPVMNS
                     SFSPNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDRYLGKMN
                     RVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLKEWAEKNFDIKKWYPDGTPLPE
                     FYSEREGMKGWNLFQLMHRKARGDEVSNDKFGGKNYCAESNGNAADTLMLCASWVAQT
                     DLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNTLASLDLPKPEQGPETINQVTE
                     HKMSAE"
BASE COUNT         3635 a         4768 c         4223 g         3986 t
ORIGIN      
        1 taattcttaa gaacccgccc acaaggcggg tttttgcttt tggatctgac aataaccttc
       61 acgaaaaaaa ttagcttata aagtctgggg gaattactct cgccacgtta acgagagtaa
      121 ttttattgat attaatctcc tgatacttta cccccgtcca aactccagcc gctgcacatt
      181 caccatccca ggcttctcag cagcactgac atcaatttgt gtcacccgca gcgcatattt
      241 ttcatccagt gcgtttaacc atttcagcag gtcattaaac accacaggtt ctatccagac
      301 ctgaatattc tccccacgat cggctatccg cctgatgacc accgagtgcg cggaagcact
      361 gtcactgatg acccgcgata cctgcgcagg cgttgttgtg gcagattttc gcgctgcaat
      421 aatatccggc gcggcgttct tcagtcgggt gttcatcgcc accagctgct gcaatattgt
      481 ctcctgttgc tcaatccgtt cgctcaacgg ctgccagatg aggacgtaat atccgacgct
      541 aaacaggaac accaccgctg ccagtaacat gcccttttca cgcggagaac gccccgccag
      601 gtgttgtgcc agccagtgtt cgccacggct taactggcgt tcacgccatt gctgaaaata
      661 gtgaataaat ttatcgcgta acatgttatt tcctccgcaa cgttacgccg ccggaaaccg
      721 catcaccctc tttctgtaac gcgtcctgtt gcacaacata atcggttgcc agtgcactac
      781 gcagtttgtc gaaactggca aagttcgcgg cccgtagctg gaggtgaagt atctggcgtt
      841 tttgatcaaa ggtaaaacca cgcatttcga tgtcggaaag tgacgctgat ttcagggtgc
      901 tggctatcgc tgacaactcg gcgagcagcc gggtatcgtc ggcctgtggg cgatattttt
      961 tcagcgccat cgtcacctga gagcgtaaat tcacaatccg cttctgctcc gggaacaacg
     1021 ttaagaactg ttcctccgcc tgggtgcggc tttgcgccac ctgttcgctg acgctccata
     1081 acgtcacgcc ccgctccact gccagcgcaa ccagaatcag caatatcggc agaatcatca
     1141 cccgccagcg cgcccactgt tttcggtagc tgacgcgagg ctgccacggc cctgtcagca
     1201 ggttcccttc cggttcgcca taagtggtaa tggcgggcag agctgtaacg gtcaggcgtt
     1261 cagcgtctgc gccagcccat gctgatagct tttccggtgc aatgccgact acggttagcg
     1321 aaagcggtaa atcctgctca ttgagctggg cgcggaacat gaccggagcc agcgccagcc
     1381 cggcgctcca tccccggcat tcatcgatgc ggcagataac ccgttgcgca tcgcaagcca
     1441 taaacccaca aggaatggac atccagtccg gcgcgacggt agcgcgggtg atgccgtttt
     1501 cctgcaacca ctgcgcaatg ttgcgcatat gctgttggtg aatcactgct acggttgcca
     1561 gttgctggtc gatattcaac ggggcgaaat gcagttcatc gatatcctgg ttcagctctt
     1621 cttccagtaa ggcgggcaga atagtcggta tctgcttgcg ggcgacatca ggcagttcaa
     1681 cctgccagac gctgatccat tcgccgggaa tgtagagtcg aatcgcatcg gtttgcagcc
     1741 attgctgaag ctgttcgtca gcaacgtcag gccagatacc gtgttccacg tcgggtgtac
     1801 ggcgctgcca gcggacggta tcggaggcgc aaagcgggaa aaaaatctca aggatggaac
     1861 tcactcactt tctcctgtct gatgccagag aaccgaaaag tgttgtgggc ccatgcggac
     1921 aattaacgag ttcatcgtca gttcaatctc attcacggtg atatctgaac gcagccagaa
     1981 gtaattgctg tccacgctca ggacggtttt tagctgtttt ttagtacgct catcgacgtc
     2041 agcaagtagc ggctgtgcaa gaaactgatc gacatcttcc cagcccttcg ccggacgttg
     2101 ttgtaataac gcccgcgcct gaacagggct taaccacggg tcaaacagcg cctcaagaat
     2161 cacactttgc gtgacgtcta aggtattgat gttgatttgc tggcgggtca tcggcagcgc
     2221 acagaccagc ggtttcagtt tttgataaag cccggcgtcc attccctgca ccacgcgcat
     2281 ctcgctgata tcggccagcg gttgattggc ggcgtagaac ggcaccgagc gggcgagata
     2341 ctcgctgtct tcacggccca gacgcgtctg cacgctgcgg tcctcgtcaa taaactccca
     2401 caggctttcg gctatcagtt cggcccgata agcaggcaca tcgaggcgcg agatcagggc
     2461 aatcaactgt tgtaccgcaa gcggacgcga cgccgttgtc ggctgagcaa gggcattcag
     2521 gttaaagcaa gcctgtgcgt cacgcagggt gaccgcaatt tgcccctgcg gcagagggaa
     2581 gaaacgaggg ccggatgccc acggctgtgc cagatgcacg cgcttttcat ttttcaggct
     2641 cagactgagc gcgctcagcg ccaggctttc cgcactggcg ctgtaccaca gcgcctgctg
     2701 gtactcctgc tggctgcgcg ttcgcccaag ttgttgctgc atccgcccgg aaagcgtgat
     2761 ggttaccagc atcataaccg ccagcaatac cagcaccacg accagtgcca ttccgcgttt
     2821 tggtggtgag gtgatcatga ttattgcggc ccgcgtaaca accagatgcg ttcaatctcg
     2881 ccccattgcg gcgaatgcag ggtcatgcgt accgccacag ggatcgcctg tactgacgac
     2941 cagctctcct gccagcgcgt gccgtcgtag aactgcaaac ggagcgaatc ggccggaatc
     3001 aacttttgca ttgtcggctt cacgctgcct gcggcatcgg tcagcggcca ggctaaccgt
     3061 tcgagataac cgtcatgaat gcggtaaccg acggtgagca gattactgcg cggcagacgc
     3121 atcaacgggt taaccacacc gccgcgcaca aaacgcattc cttcactctc agacgccagc
     3181 acgccagcgc cagccagtaa agctggttca cgctggccct gatcgcctct taccggacgc
     3241 ggcatcattt gggtcagatc gtgggtcaga aaactcatcg tttgctgcat gaggtttagt
     3301 ttttgatcgt gatcggcgac ggcgctattc acgcgcgtga cgccgttagt cacctgctgc
     3361 gccatcagtg ccagcgaggc aaaaatggca attgccacca acatttccag taacgtgaaa
     3421 ccagcgcgag tccttctcac tgttggcctc ccacggcgct aaaccaggcg cgtcgtgact
     3481 gaatgaccga cgaaaagtct tcgtgaaggc tgacttcaat atccaccgcc tggagcagcg
     3541 cgttgcctgt actcagcggt gtggttcgcc agtaccagcg gcttcctgcc atatcgctct
     3601 cgccctgctg cctgaccgcc cgctgcccac gggtcatcag gtagagcagc gcagtctggt
     3661 tttcagccac ccagcccgct accgtttttt cctcaagaac gtgctgatta ctcagcgcac
     3721 cgctggcaat ctgtaatacc gccgtggcag ccagcgcaaa aatcgccagc gcgagcatca
     3781 cttccagcaa ggtaaatccg cgcttcatgg catcctctca tcacattgat tgagggataa
     3841 cgcgccatcg tgcgccagtt ttaccgccca acatgcgttt tgcgccgccg agtagaagcg
     3901 cagcgtaaac ggcgtggcgg gttcaaaagg cgaaaaacgg atctgcggcg tcttcttttt
     3961 cgcctctttt tgcagctcca gttcaatatc gtgcagcgtc aggcgacgac gttgcagctc
     4021 cagcgcatac tccttctgcc agatatcact tccgggttgt aacagcatct gcacctgttt
     4081 tggcaccgta acctgtgccg ataaccgggt cgcggaaacg ggcaaccact gcccctgacg
     4141 acgctgcata aactgatagc caggcgcgtc gattagcaca ccgagtgtct gcccttcaat
     4201 cactgccctg tccttaaact gcgcaaagcg agtcagaaaa tcctgtgccg cttttttcgc
     4261 gggcggttca gaatcggtcg caaacgtctg taccacgccc gaactggcaa ggccgataag
     4321 gaaaatcacc aacatgattt ccagaagtgt gaatccgcgt tcaggcactg cttactgaaa
     4381 ctcctgcaaa ttccagttac cgatatctgc gccagcgcct tccccgtttt cctgaccatc
     4441 cgcccccagg gtataaacat caaacagccc tttttcaccc gggctgagat actgataatc
     4501 attgccccac ggatcctttg gcagtcgttt aatgtatcca ccagtacggt agttgcgggc
     4561 gtccgccata ttggctggct gctggatcag cgcctcaagc ccctgttcgg tagttggata
     4621 acgcccgtta tccagtcggt acatatccag tgcgttttcc agcgccacga tatcgctgat
     4681 ggctttttgc cgatccgctt tctctttatt gcccaacagg ttaggcacca ccagacttgc
     4741 caggacgcca agaataacaa tcaccaccat cacttccagc agggtaaaac ctgcccgtgg
     4801 tttttgtgtg cgggataacg aattcatgtg ataactccgt aaattacatt ccaaccatat
     4861 tgttcagttg cagcatcggc tcgaggatgg cgatgacgat aaacagcacc acgcccgcca
     4921 tcatcaccac cagcgccggc tcaaacagcc ccaacgccag ccccacctgg gtatcaaatt
     4981 cccgctcctg gttaactgcg gcctgttcaa gcatggtttc cagttcgccg ctctgttcgc
     5041 cggaggcgat catgtacagc atcatcgggg ggaacaggcg caactccgcc agcgcagcgc
     5101 gcagactgct tccttcgcgg acgcgatctg ccgccaacag cagttgttgc tcgacatagc
     5161 gatttgccga cacggcggca gcggtctgaa tgccttccag tagcggaaca ctgctggcgg
     5221 tgaggatgct taacgtgcgg gaaaaacgcg ccgtattcag tccgcgcgca acacgcccta
     5281 tcacgggcag gcgcagcaag gttttatccc agcgcaggcg catcgcagga tttttaagta
     5341 gccgttgccc cagcaccaga agccccagca aacccgccag ccagtaaacg ccgctggcct
     5401 gcaacgcatc gctcatggca ataagcgtgc gggtggagac gggcagcgcg tgtccgagat
     5461 gatcaaactg ttcgatgatt ttcggtacta ccgccgtcag caaaatagtg accacgcccg
     5521 ttgccaccac cagcagaacc agcggataga gcatggcctg caatagacgt gatttcaggc
     5581 gctgtcgctg ttcggtgtaa tctgccaggc gattaagcac cacgtcgaga tgcccggatt
     5641 tttctccggc agcaaccatc gaacaaaaca gagaatcaaa gacgcgggga tgttcgcgca
     5701 ggctgtccga cagggtgtaa ccttcctgaa tccggctgcg cagcgccatt ccgaggcttt
     5761 ttacatgcag tttttcactt tgctcactga ccgcctgtaa gcaggtttcc agcggcattg
     5821 ctgcctgtac cagcgttgcc agttggcgcg tgaacagcgc aagatctgcc gccgccacgc
     5881 gacgatgtgc gtgccgccga cgctgcaaca tcccccctga cgaagtattc atccgggctt
     5941 caatatgcac ggggataagc tctttaccgc gcaacaactg gcgggcatga cgcgcggaat
     6001 ccgcctcaat catacctttg gttttgcgac cattacgctc cagcgcctga tagtaaaaca
     6061 gtgccattac gcctccatgg ttacccgcag aacttcatcg agagaggttt ctccggcgag
     6121 cactttctca atgccgttgc tgcggatacc cgcagagtgt tgtcggacat aacgttccag
     6181 ctccagctcc ccggcctgac ggtggatcaa atcacgcaat gtggcatcca ccacgatcag
     6241 ctcatggatg gcagtccgtc cgcgaaaacc tttgtgatta caggcgggac agccctgtgg
     6301 atggtacaga gtgacggtac gggcgtcggt aattcccagc aggcgttttt cttcgtcagt
     6361 ggctggtgcg ggctggcggc agtcgggaca cagcgtgcga accagtcgct gcgccatcac
     6421 gcccgtcaga ctggaagaga gcaggaaagg ctccacgccc atatcctgca aacgtgtgat
     6481 cgcccccacc gctgtgttgg tatgcagcgt ggaaaggacc aggtgtccgg taagtgaagc
     6541 ctggacggcg atttctgcgg tttcggtatc gcggatttca ccgaccatca ccacatccgg
     6601 gtcctgacgc aaaatcgcgc gcagcccacg ggcaaaggtc atgccgacgc gggtgttaac
     6661 ctgcgtctga ccgatccctt caatcatgta ttcgatggga tcttcaaccg tgagaatgtt
     6721 gcgcgaatgg ttgttcagct cctgcaatcc ggcgtacagc gtggtgcttt tgccggaacc
     6781 cgtcggcccc gtcaccagaa agatgccgtg cggtttgtgt aacagctggc gcaactgcgc
     6841 ggtcagttgc tggctaagcc ccagacgctc cagcgtcagg cgggcctggt ttttgtccag
     6901 cagtcgcagc accacgcgct cgccccaggc ggatggcatg gtggagacac gcacgtcaat
     6961 cgcccgaccg cccagcagca gcgcaatacg gccatcctgc ggtacgcgct tttcggcaat
     7021 atccagccgc gccatcacct tgatacgcga taccagcagc gaggccagtt tgcgccccgg
     7081 gcgcagcatt tcatgtaatg tgccgtcaac gcgaaaacgg atcaccagac tcttttcaaa
     7141 cgtctcgatg tggatatccg aagcgccttc tttaatcgcc tctgccagca tggcgttgat
     7201 cagtttgatg attggcgcgt cgtcgtcgct ttccagcaga tcttccgttt ccggcagttc
     7261 ttccgcgaga gtaaaaaagt cctcagcaga accgagatct tccatcagct ggcgggcctc
     7321 ggaagagtcc cgctgccaga ccgcattcag ccgctgttca aattcggccc cgtcgatttg
     7381 ccgcagcgta aagggcgcgt tcagcccccg ctgcagctcc tgcaaaacag agagcgacaa
     7441 cggatgaacg tggaggatct ccagcgacgc ttcgcaccat gccaccaggc taaaccgacg
     7501 gctgaaactg tagggcagac gcacggtgtt gacggtggtt tcctgtgcta caggcaccat
     7561 taacgcgttc tcccggcatt gaggaatgcg cggacttccg gcggtaaggc ctggttttgc
     7621 gctggcagta ccggctgtgc ggtgtgcggc atcaggctta agccttgctc atcgcgatag
     7681 atctgttcgg cgcgcatata gttatatttg cgctgcgaca caccgtctgc cgccataccg
     7741 tcacgcagaa tggtcgggcg gataaacacc atcaggttac gtttttcttt tttatccgcc
     7801 gtcgatttaa acaggttacc aatcaatggg atatcgccca gcagcggcac tttcgccacg
     7861 ctttctcccg cctggtcgtc catcagaccg ccaagcacaa tcagctcacc atcgttagcc
     7921 agcacggtgg ttttcagttt gcgctcgcca aacaccacgt cgaggctggt ctgtccttcc
     7981 accttcgaga cttcctgctc aatcaccatc tgtaccgcgt taccttcgtt aatctgcggc
     8041 gtgactttca gcatgatgcc gacttttttc ctttctactg tattgaaagg attgctgtta
     8101 ttggagccaa cggtagagcc ggttaatacc ggaacatcct ggcctaccat gaagaaggct
     8161 tcctggttgt ccagcgtggt gatgctcggc gtggagagta cgttagaact ggagtcgttt
     8221 ttaaccgcct gcaccagcgc catccagtcg cctttcacca cgccaaccgc cgtaccgctg
     8281 aatccagaaa gaagctgggc gagtgtggag agatcgccgt tggtatccgg attaatggtg
     8341 gtagcgccgt tttcactgat caccgtggag cctttctgcg gtttcgcctg agaaatggct
     8401 gcccccagcg tgccaatagg gatctgcgta ccgttagcaa actgcattaa tccggcatct
     8461 ttcgacgccc actgcacgcc gaagttgata ttactgcctt cggcaacttc cacgatcaac
     8521 gcctcgacat gcacctgagc acggcgaata tccagttgtt caatcacgct ttgcagcgac
     8581 tgcatgatgt cctgcggcgc ggttacaatc agggcattac tgtgtttgct ggcggcgatg
     8641 gagacaacct cacgcccgct accgaccgtg ccttccgcct cttctttagc cgccgtgagc
     8701 gtgccgctga cctgcttcag cacatcgacc agatcttcgg ctttgctgta tttgagatag
     8761 aaaacctggc tgttgccgct gcgttccatt tctgagtcca gccgacggat caggcggcgc
     8821 attttgtccc gcgtggccgg gtcaccactg acaatcacac tgttggtgcg ttcgtcggcg
     8881 acaatttgag atttcagtgt tgctggttgg ttctcgccgc tgtttttagt caggctttcc
     8941 agcacgcggg caatttccga agctgaggcg ttatccagcg ggatcacctc ttcagtgcgg
     9001 tttcccgcat gatccacgcg ctggatcacc tccgtcagac gctccacgac tgaggcgcgt
     9061 ccggtgagca taatcacgtt ggagggatcg taattaacaa cgttgcctga gcctgcgctg
     9121 tcgatcatct ggcgcaaaat cggcgccagc tcgcgtaccg acacattgcg taccggcact
     9181 actttggtaa ccatttcatc gcccgcgtag ttgtcgctgc cttcaccgac cagcggcagc
     9241 ggctcgactt tcgcggcgct ggatttcacc accttcagca cctcgttttc catcggtacg
     9301 acggcatacc cctgtgcttc cagcaggtta aggaatagct ggtaatactg acgttcattg
     9361 agcggggtca tggtgcgaat actcactttc ccctgaacgc ccggccccat aatgatggtc
     9421 ttattaaggt tagcgccgac ggtttcgatg aacgatttca ggtcggtatc tttaaaatta
     9481 gcggtgaaag tggcttcttc cgcccagacc ggtgaactgc ataatgccgc tgccagcacc
     9541 agcggcagta aacgcttttt tgttttgagg ccagttgtct tcttacgcca gaccgacaac
     9601 gtaatatcac gccaaaacac gatgaatgat tctcctggat taaatgcggt tagcgcagcg
     9661 cgatggaaat gtcgtggcgc gcacccttgc gtaaaaccgt aagttgaatg gaatccattg
     9721 aaggtaactg ccgcatcaga gcaatcattg ctcgtggatc agtgaaatcc tgctgattta
     9781 gcgcaatggc gatatcgcct tccttgaaac cgctggcatc gaacagagaa cgatctgccc
     9841 ccggcttcgc tgcataaccg acaatcccct ccttacgcac aggcgtaagc tggatatagt
     9901 taaaaatttt ctgcggatct ttcgccagtg cctgacgcac ggcagccggg atctcaactg
     9961 gcacactgac agcaggctca gcaacagctt gctttgcttc gtcactgacg gcttttttgt
    10021 tggtcacagc aacagtggaa cgttcctctt ctgccagact cagacgctct atttttccct
    10081 gataacgcag catcacatgg tcgcggttga tttcctcaat caccgcgttg tgagagccaa
    10141 gcgtttcacc ctgcaaatag acctgctgtt taccgccttc ttcaataacc gcgccgggtc
    10201 tggcaccaaa ggcgatccca cgcagcacca cattaagacg cgtttctgcc acaggcacag
    10261 gttcgggttg ttttacctgc gcggcgacag gctgatattt gccaaaccag ttttgctggc
    10321 tgattaattg cacgtcattt ttatcgaacg tttttgcatc cacacggctc ggtttattca
    10381 ccggttgaga aaccgccgta tattccgcag aaaaggagat atagcgccag agtgaatgcg
    10441 ccatttttgc agaaataata agcagcatca gccagaacat cccgcgtgca atttttcgca
    10501 gatgctcttt gtctgtatta agagattgtc tctggttaag agtgtgacga atttttgtca
    10561 gccattgaat gagataaatt cgtgcgtcac gaaaaacaac ccgcgccaat ttatatctac
    10621 ccgacgttat gctttgacta ttccacaggt ggtacgatcc agtttccgct gatactggtt
    10681 acctgtacgt gtatcattaa tcgttatgct gtaattaatt ccctcagtga tcattaattt
    10741 caccgtcggg tcagcgcaca tttgtcgttg atagctggtt aaaaaggcgt caggcgtctg
    10801 cgtggtttgt gtacccgctt cgctgataat ggtcattttt accgttgtgc cgctactttg
    10861 cgccagcact aaggtatatc ccgcagattt aatcggcagg ttttggctga tattttgcgc
    10921 ctgttttttc gccagtaaac tggcattttc attatggctg gcacagccac ttaataatcc
    10981 tgtaacgctc aacaatagcg atattaatac cctccctggc atttgtttta tcgacatggt
    11041 aaataatctc taaggttatt aataagagtt aaaatgtcac tttgataatg acgtggttat
    11101 cattaaaaca atgcctgtag ataaagtgtt gctataccgc ccagacttaa acacggtcca
    11161 aaaggcagtg tggttgatcc tctttttgta ataacggcat atatcaggcc gcagcatgag
    11221 gcgattaaag caacattggg cagcgacaat gcccccaccc agccacctaa cgcggcgaaa
    11281 agtaatacat cgcccatgcc taatgcttct ttacgcagaa ctattccggc tatccaacgc
    11341 agagagtaaa aagtgataaa tcccaccagg acgccggtga ctgcatcttg tagcgtgagc
    11401 ggactctgct gcgcccatgc cgcaatcagt cctgtccaca atacgccctg agtaaaaaca
    11461 tcgggcagcc attggtgatc gaggtcgatg acgctcgcgg caatcagcca ggcggataat
    11521 atcatcaccg ccagccccca tccactttct ggccagacca gactcgccag caaaaaagca
    11581 agtgctgtca ataactccac cagcggataa cgcttgctga ttttcgcctg acagtcgcgg
    11641 cagcgccctt tgagcatcag ccaggagaac agcggaatat tgtcacgtat ccggatggtc
    11701 tgctgacaat gtgggcagtg cgaacgcggt agcgcaaggc ttatttttga ctgcgcactc
    11761 gacatttcac cgtgaaactc cgccatttgt tggcgcagca tgattgggta acgccaaatc
    11821 accacattca aaaaactgcc gatgatcaat cctccgacgg ttgccaggac gggcatcgcc
    11881 gtggggtatt gctgaaaaac atcaaaaagc atggttaaag gttgtttgtt gtaacttgct
    11941 ggatgcggcg taaaacgcct tatccgtcct acgggtgtct gccagcgcaa ataatcgcgg
    12001 ctttccccgt ctgtaggccc gataagcagg cgcatcgggc aaatgtgtta acccggtgcg
    12061 ccttatttca tgccggatgc ggcgcgagcg ccttatccgg cctacgggct tactcggcag
    12121 acatcttatg ctcggtaacc tgattaatgg tttccggtcc ctgttccggt ttcggcagat
    12181 cgagtgacgc gagcgtgttg taagccgact ggctcacacc gccctcgaag ctcatctcgc
    12241 tcgcccccgg cagctggtaa gcattcgcgc ccggattcca tttcttaaag aactccgaaa
    12301 gatccgtctg ggcgacccag gaggcacaca gcatcagcgt gtccgctgcg ttaccgttgg
    12361 attcagcaca gtaattcttg ccgccaaact tgtcattgct gacctcatcg ccgcgtgctt
    12421 tacgatgcat caactggaac aggttccagc ctttcatccc ttcacgctcg ctgtaaaact
    12481 ctggcagagg agtgccatct ggataccatt tcttgatatc aaagtttttc tctgcccatt
    12541 ccttcagctg tgcgtacatc agcagacggt cacccgcacc gccgcgtgcc catgcctggt
    12601 tgttgctctc ctccagatat tccggtgcga cggtaatatc gtcagcgaca cggttcatct
    12661 tgccgagata acgatcctgc atgtacagcg ccagcacgtt gttagcgact tcagttgcac
    12721 ccggtacagt caacggcgtt tctgcggcgt tatgaccgac ttcatgccag atcagccagt
    12781 cgttcagcgg cgtcgtcggc agcgtggtgc tgttcggcga gaagctgctg ttcattaccg
    12841 gataacccga atgcgcatca ccgatggaga tctgcacatc gttggtgaaa cgatgtttgt
    12901 ggcccggcaa gtttttatag gtaaacatcc ggtgcttacc gtcttcgcta tcacggccgt
    12961 agaagtcatt catcgagctg gcaaaggtat ccagatcgtt agcgaattgc tccagtccgc
    13021 cagtgtaatt gctggcattc aggttcttct tcggtgtggt atagacgaaa gcgtctgatt
    13081 ccagctcacc cagcggagcc ggtgagttca gatcgttttt ccatgcgccg tctttataga
    13141 acggtgcttt taccacgcca gtaaaggtga agctggcaga ttcattggta gagctattgc
    13201 ccttgatata aatcaggcca ccgtaaggca ccttgaactt caccgtaccg ctagcgtcca
    13261 gagagtacgt tttagtcact cttggcggac ggttcagcgc aacttcatgc ttctcacgtc
    13321 cggtcaggtc gtcagccagc gccacggtga cggtcacagg aacgttcgca ttggacttaa
    13381 tggtgacctc tttctgagcc ggtgcccaca ggccagttga ctgcatgtta cctgcaaacc
    13441 atttggtcgg attcgagtac aggctgatgg tttcagtaac gttctgtccc tcttccgata
    13501 ctgctcccgg atacttctcg acatcaactt tgatgttcag atcccaccag gaacggccca
    13561 gcatcaggcg tgtcagcggt ttttccatat agttgagcgg gtagctcggg ttcatcatgc
    13621 ccgctttgct gctaccgtca ccgtagatca tgttgttatc gaccagcgat tttttcagat
    13681 ctgcagaaca cttggtgccg cctgcatagg catcattggc gtagcagttc aggaactcgg
    13741 tgaacgtttt aaagcccagc tcgtcatttt tgccttcttc ataacgatag ctcgtatcgt
    13801 tccacagcca gaccgacatg ttctggtaca gacgttccag atcgacgctg ctcagacgct
    13861 cacctttgcg accattggtc cggaagtaga gctcatgctg atacagacgc tgaatgttgg
    13921 tgcctaaatc cgcagcctgc accatcgctt ttgcagtgtc ggcgttaagg cttagttgcg
    13981 tatactgtgg aacatacatg ccaccagtaa ccggaacccc cgtgccagga cgatattcca
    14041 ggcagttgac ctcatagtga tatgccggat tagtacactc tttcagcccc gggaacgcgg
    14101 cgaaaatttt ctccttcgca gccttcagag aatcctctgt tttatgatcg gcctcatcaa
    14161 taaaggcata acgcgtttcc tgtttgccat ctacatcttc cagccagctg gcaacttcca
    14221 gcttcggttt gtcatcaggt ttgttttcta cctgatattt ccacttaact tcccctgtct
    14281 tactatcgat ggtgtacggc agcgcaccat ctacggcagg ataacgttca tagacccaaa
    14341 tgcccgttgc gcgctgctga cgaacgcggt tcggataccc ttgcggatcg ttatttacta
    14401 ccgacttgtt cagtgccatc gacagacctg cggcatccaa cagacgcaca aaaccagacg
    14461 cgctctcttc cttaagattg ctcatcacgt tttccatgat cagcaccgat ccacctttgt
    14521 tcagataggc gatcagatcg gtcacatcct gctgagtcag cttcggtttg ctggtatctg
    14581 cacgcagcgg gattgcataa ggatcgttac ccacctgagt cacatattca aagccgttaa
    14641 ggatcagcag cggcatttcc tgcggatcga gatcgccata gctacttaaa tgctcaacag
    14701 agatgcccgc aaaatccgga tggaagtcga acgcagcgct gtttcctgta acctgaccat
    14761 gacgtttaaa atagacagta tccaggttgg tgcctacggt catgctggct ttcgcgtccg
    14821 gcttccattt atcgtcggac agatagcgca gcacgttctc catgaagttc ttcatgtcat
    14881 ccgggtcgct gttgagcgta cactgcccat ctttattaac gcccccgttc cagctgtaac
    14941 cgttcgggca acgcaaaatg ctgttgtagt gtgggttacc gataaccatc agtttgccct
    15001 caccgacttg ccccagcgaa ataaacggca ggttaaaggt ggcggtatcg cgcgtaacgt
    15061 tttccggctc aacaagagaa ggcgcttccg taatgtacgc cagctcgttt ttatcccagg
    15121 cgcgtttttc gccgaaggcc agccagtagt ttttatcatt acgcgccatc agaatcggga
    15181 aggccgcgtt ggagatattc accaccgcct gaccgcgcgc attacccgtg ctgccataga
    15241 agttggtgga gtcatggaat acatggaact tgctgacaga tttgtagttc gtatccacgc
    15301 cccacagctt gttgataacg ccctgaatct ggccgtcatt aacattgcgc gtcgtcagcg
    15361 agaaccagcg agcctcgtta caaccatcgg ttttcgcaca aatcgcggta tcgatctctt
    15421 tggcctgacc cgtattaaac tgctcaataa attcgttagg cagattaacg acttgctcac
    15481 cttcccccag cgtcgcaccg ttggataacg agagattgat aatctcgttg atcacgttgg
    15541 gatattcggc aaagaccttg cgtacatcgt ccggaacaac acgggtatta ttttgcccgg
    15601 tcgtcgaata gcgatgaata agctgatcaa tattcgcccc gcgaacttca tcacccagtt
    15661 cagtcagcgc aatggtcgac ttattgccgc gcactgaacc cagttcaaag gtatcgatac
    15721 caaaggagat ggtttcgccc cagctaaagg aaaattcacc attttcccct gtcacgccac
    15781 ggcctgaatt ggtgtagtag ttgacgccag caacaccata tccctggcta tcgaccagtc
    15841 gaccttcaga gagaatgatt tcagtgggtt gatactgata aaactgttcc gcgttagccg
    15901 acacgaagga agcgttcaga tccggttttg ttcccggcgt ggtgacgggc acgaccggtg
    15961 aagtatgagt ggatggcgct ttatcggtcg cagcattgtt ttccacctct tcattgacca
    16021 gctttttgaa ctcttccggt gccagatcga tttgcttata cagcgagtcg aagcgtttac
    16081 tctcgatcac cgaggagaac gtcagacaaa cctgttctgt attcgccgga cagctgttac
    16141 tggacgttac cagcgaaacc gcattgcttt tcttgtcatc ggagcccgcc agttcttgcg
    16201 cgtcctcaag gctaaacgac actttttcaa ccgcacgcaa gctacgcgca gcttctgact
    16261 gagtgttgaa ggtggcaatt gtcgtgttac ccgccacgca agtaacgtcc tcgccaggtt
    16321 taaatgtaaa gccatcgctg gattcaccat tacaggtagc accagttacc cgctggcttc
    16381 cgcccagggt cagataaccc gttttcgtag gaacaggttc tggctccggt tctggtgttg
    16441 gttcaggatc aggtatcggt tctggcgtag gctctgggtc cggcgttggc tcaggcgtcg
    16501 gctccgggtt tggtgttgga tcaggtttca cttccggcaa agaccctgtt ccagaatcta
    16561 caggcggcgt atcggaggaa gatccggaac caccgccatc acaaccggct aa
//
LOCUS       DBBGLQ010000093        16597 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.093, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000093 DBBGLQ010000000
VERSION     DBBGLQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.093"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(17..169)
                     /locus_tag="ACIYSB_004031"
     CDS             complement(17..169)
                     /locus_tag="ACIYSB_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGC5430965.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(434..1168)
                     /gene="cysH"
                     /locus_tag="ACIYSB_004032"
     CDS             complement(434..1168)
                     /gene="cysH"
                     /locus_tag="ACIYSB_004032"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGC5430966.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1242..2954)
                     /gene="cysI"
                     /locus_tag="ACIYSB_004033"
     CDS             complement(1242..2954)
                     /gene="cysI"
                     /locus_tag="ACIYSB_004033"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGC5430967.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPARPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2954..4753)
                     /gene="cysJ"
                     /locus_tag="ACIYSB_004034"
     CDS             complement(2954..4753)
                     /gene="cysJ"
                     /locus_tag="ACIYSB_004034"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGC5430968.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5072..5434
                     /gene="queD"
                     /locus_tag="ACIYSB_004035"
     CDS             5072..5434
                     /gene="queD"
                     /locus_tag="ACIYSB_004035"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGC5430969.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5512..6783
                     /gene="ygcN"
                     /locus_tag="ACIYSB_004036"
     CDS             5512..6783
                     /gene="ygcN"
                     /locus_tag="ACIYSB_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGC5430970.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6774..7034
                     /gene="ygcO"
                     /locus_tag="ACIYSB_004037"
     CDS             6774..7034
                     /gene="ygcO"
                     /locus_tag="ACIYSB_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGC5430971.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GNLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7051..7626
                     /gene="ygcP"
                     /locus_tag="ACIYSB_004038"
     CDS             7051..7626
                     /gene="ygcP"
                     /locus_tag="ACIYSB_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGC5430972.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7774..8634)
                     /gene="ygcQ"
                     /locus_tag="ACIYSB_004039"
     CDS             complement(7774..8634)
                     /gene="ygcQ"
                     /locus_tag="ACIYSB_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGC5430973.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8631..9410)
                     /gene="ygcR"
                     /locus_tag="ACIYSB_004040"
     CDS             complement(8631..9410)
                     /gene="ygcR"
                     /locus_tag="ACIYSB_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGC5430974.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9388..10797)
                     /gene="ygcS"
                     /locus_tag="ACIYSB_004041"
     CDS             complement(9388..10797)
                     /gene="ygcS"
                     /locus_tag="ACIYSB_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5430975.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10819..12273)
                     /gene="ygcU"
                     /locus_tag="ACIYSB_004042"
     CDS             complement(10819..12273)
                     /gene="ygcU"
                     /locus_tag="ACIYSB_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGC5430976.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKVTGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12343..13128)
                     /gene="ygcW"
                     /locus_tag="ACIYSB_004043"
     CDS             complement(12343..13128)
                     /gene="ygcW"
                     /locus_tag="ACIYSB_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGC5430977.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13447..14724
                     /gene="yqcE"
                     /locus_tag="ACIYSB_004044"
     CDS             13447..14724
                     /gene="yqcE"
                     /locus_tag="ACIYSB_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGC5430978.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQIAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLTLTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14751..16229
                     /gene="ygcE"
                     /locus_tag="ACIYSB_004045"
     CDS             14751..16229
                     /gene="ygcE"
                     /locus_tag="ACIYSB_004045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGC5430979.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDNMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3959 a         4373 c         4170 g         4095 t
ORIGIN      
        1 tacccccgcg tggtggctac ttcttcgatt cgtaagcgag aactgcctta aactccatac
       61 cacgttctct gatactcaac tcacacaacg agtcgcctac catcagcgtg aatcccagta
      121 ctgtaaaaca cagcactatg attgccacaa gggcatattt tgtcagcatg tcttgcctcc
      181 ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga acgtaagcct caggttgata
      241 gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg aatgcttaac gccagacagc
      301 ctcaagcacc cgacgccatt ctatacctga taattcttcc agcggcgttc acgtttaaaa
      361 tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt tctgacagag gcgtttaatt
      421 tatccggcaa tatttaccct tcgtgcaatc cacattccct ttttaagcca aagaaacgtg
      481 tttcttcttc cgacatgcca ggttcccatt tacgggttgt atgggtatca ccgaccgata
      541 aatatccttc atcccataat gggtgatatt tcaggccatg tttttgcagg tactgataaa
      601 tagttcggtt atcccagtcg ataatcggca gcactttaaa tacgccacgc tgaattgcca
      661 gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg caggccagca aaccaggttt
      721 gcgcgttaag ttctttcagc gcccggttca tcggttcgac tttgttgatg tcattgtact
      781 tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta gcgtgcttcc tgccaggcgg
      841 cgctttcggt agcacggtac actttcaggt tgagcttgag tttgtccgtt aactcgtcaa
      901 taaagcggta ggtttccgga aacagataac cggtatcggt gaggatcact ggaatatccg
      961 gccgaatctg attcaccaga tgcaggctca ccgccgcctg aatgccgaag ctggaagaaa
     1021 gcacatattc accgggcaga ttatccagcg cccaggctac gcggccttca gcgtccagtt
     1081 tttccagttg ggcgttagtt tccgccagcg ccagaatgcg atctaccttc ggcagttcgt
     1141 tcagggcgtt tagatcgagt ttggacatag attcctcact gtttgccttg cctgatgcga
     1201 cgctcacgcg tcttatcagg cctacaagac cgggctgatg gttaatccca caaatcgcgc
     1261 gccggatcga gcaccgggcg aatgatgccc gcacgcaccg taaagtcgcc gaagccttca
     1321 cccgcttcgc gctctttcgc ccagcgccct atcagttcat caagcgacgc caggatttcc
     1381 ggctcggtga tgttttcttt atacatccga gggatacgtg tcccaatgcg gttgccgcca
     1441 agatgcaggt tgtagcgacc cggcgcttta cccaccaggc ccacttccgc cagcatcgcg
     1501 cgaccacaac cgttcgggca gcctgttaca cgcatcacga tatgctcatc gctgacacca
     1561 tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg gcaggaaacg ctccgcttcc
     1621 gccatcgcca gcgggcaagt cgggaatgac acgcaggcca ttgagttttc acgctgcggc
     1681 gtgacggcat tcattaaccc gctctctttg gcgatcttct cgatcttcgc tttctcgctt
     1741 tccggtacac cggcgatgat cagattctgg ttcgccgtaa tgcggaaatc gcctttgtgg
     1801 atcttcgcga tctccagcag gccggttttc agcggacgcg ccggataatc aaggatgcga
     1861 ccattttcga taaacagcgt caggtgccag ttatcatcaa tgcccttaac ccagccaata
     1921 cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt caaatttgat ccccgcgcga
     1981 cgctccactt ccgctttaaa cgtctcaacc cccacgcgct ccagcgtgta tttggttttg
     2041 gcatttttac gatcggttcg gttaccccag tcacgctgag ttgtcacgac ggcttcggcc
     2101 accgccagcg tatgctccag cggcagatag ccaaactcac tcgcggtgcg ggcgtaggtt
     2161 ttcttgttgc cgtgttcgat ggaaagccca ccgcccacca acaggttaaa gcccaccagc
     2221 ttgccgtttt cggcgatcgc cacgaagttc atgtcgttgg cgtgcagatc gatatcgttc
     2281 tgtggcggga tcactaccgt ggttttgaat ttacgcggca ggtaggtctg gccgaggatc
     2341 ggttcttcat cagtagtggc gactttttcc tggtcgagcc agatctccgc atacgcgcgg
     2401 gtacgcggca gcagatgctc agaaatcttc ttcgcccact cgtacgcttc cgcgtgtagc
     2461 tgcgactcgt aagggttcga ggtgcagagt acgttacggt tcatgtcgtt agcggtcgcc
     2521 agcgcatcaa gaccaaccga gtgcagcatc tggtgcaccg gtttgacgtt ctttttcaga
     2581 atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa tgctgccata gatggtgttt
     2641 tcaccggcaa atttgtctat cgcctgccac tgtttagtgg taatcacccc acccggcaga
     2701 cggcagcgca acagcatcgc gtggcgcggc tccagcttct gttcagcacg ttcggcgcgg
     2761 atgtcgcggt catcctgctg atacatgccg tggaagcgga tcagcaggaa gttgtcgccc
     2821 ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa tggtgccgcg caggtagttg
     2881 ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt cgaccactaa aggccctgga
     2941 tgtttttcgc tcattagtag acatctcgct gataacggcg ctctacgcgc agctcactta
     3001 aaaattcatc cgccgcttcg gtgtccatgc caccaaattc ggcaatcact tccagaagtg
     3061 cctgctcaac gtctttcgcc atgcgattag cgtcaccgca gacataaatg tgggcaccgt
     3121 cattgatcca gcgccacagc tccgcgccct gttcgcgcag tttgtcttgt acgtaaactt
     3181 tttctttttg gtcgcgcgac caggcgagat cgatccgcgt cagcacaccc tctttgacgt
     3241 agcgctgcca ctccacctgg tagaggaaat cttccgtaaa gtgcgggttg ccaaagaaca
     3301 gccagttttt tcccggcgct tcatcggcag cacgttgctg cataaaggcg cggaacggcg
     3361 cgatgccggt gcctgggcca atcataatca ccggggtttc tggattggct ggcaggcgga
     3421 agttatcgtt atgttcgata aatacgcgga cttcgccctc ttcttccacg cggtcagcga
     3481 ggaagctgga ggcaccaccg gcacgggcgc ggccttccac gtcgtaacgc accacaccaa
     3541 cggtgacgtg tacttcgttc tcgacttccg cctgcgagga ggcaatggaa tagagacgcg
     3601 gcgtcagcgg gcgcagcaga ttaattagcg cttcggcatc aagctgtgcc ggggagaaac
     3661 gcaccatgtc aacaatcggc gtcgtcgcgg cgtaatgctg taacttcgct ttatcgccca
     3721 ccagcggcag cagtgtttca ctgcgggtaa gcgtggcgta attctcaaca atgttggcgg
     3781 tgttgacggt cagttcgaag tgccactgta gcgcttcgtt cagaggcaac gttttgccct
     3841 cgacggtgac aggttcatcg cctttcagcc acagcagttc gacaagttct ttcaccagtg
     3901 ccggatcgtt ctgataccag acgcccagcg cgtcacccgg ctggtaacgc aggcccgagt
     3961 cacctaagtc aatttcgata tggcgaacgt ctttttcaga gttacgcccg gtaattttct
     4021 ggttaacaga gaggctagcc accagcggcg cgtctttgct gtacgggctg gtgtggattt
     4081 catttaccgc gccagtagcg acggattgcg aaggtgccgc gacaggcgca cgcgatttaa
     4141 gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc ctggtattca acatcggcat
     4201 cgacacggtc gagcaggcgt tcaccaccca gttccgccag cttgctgtcg aaatctttcc
     4261 cggactggca gaaaaattca taagagctat cgccgaggct aaacacggca aacgcggtgt
     4321 tttccagctt tggcgctttt ttggagaaca ggaacttatg cagcgcgacg gcttcttccg
     4381 gcggttcccc ttccccttgc gttgacgtca ctacgatgag cagtttttcg ctggcgattt
     4441 gtttgaattt atagtcgccc gcgttcacca gcttaacgtt cagttttgct gctaataaat
     4501 catcacgtaa tgcttcagca acccggcgcg cattgccggt ttgcgaggcg gagataatag
     4561 ttatacccgg catttctgcg gctggcgctg gcgtcgctgc aagcgcagca ggctgctgat
     4621 tgagtacgcc ccagaaatag ccagaaaccc aggcaagctg ggtgggagtt aaatcggtcg
     4681 tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg aagcaacgcg gaaggtggga
     4741 cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc tgtttctgcg ccctgtcagc
     4801 gcccataaaa cagaagagaa ggtaaggtta acggggcaaa cggtgtggat taaagacggg
     4861 atagcgataa ctaataacca aatcgactaa cctgttttag caatagtctt taacaacaaa
     4921 atagattaac caacctaatg aaaaacaaat gaatttagcc aatcattaag ataaatcagc
     4981 gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa cagcctttcc ggtactctac
     5041 ggcggtttta tcgtcctgta gagaaattat gatgtccacc acgttattta aagatttcac
     5101 cttcgaagcc gctcaccgct taccacacgt cccggaaggg cataaatgtg gtcgcctgca
     5161 cgggcattcc tttatggtgc gactggaaat taccggggaa gtcgatccgc atacgggctg
     5221 gattatcgat ttcgctgaac taaaagcggc gtttaaacca acctacgagc gcctcgatca
     5281 ccattatctc aatgatattc caggtctgga aaacccaacc agcgaggttt tagcaaaatg
     5341 gatttgggat caggttaaac ccgttgtgcc gctgttaagt gcggtgatgg taaaagaaac
     5401 ctgcaccgca ggttgtatct atcgcggcga atgataagag tgtgtcggcg gtcaatttcc
     5461 cttaagtaac gctatgttag ggtgttgtgt tctggatatc tggggcatga catggaagac
     5521 gactgcgaca ttattattat tggtgccggt attgcaggca ccgcttgcgc gttacgctgc
     5581 gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg aaatccccgg cagcaaaaat
     5641 ctttccggcg ggcggttata tacccatgca ctcgcggaac tcctcccgca atttcatctg
     5701 accgcgcctc ttgaacgacg catcactcac gaaagccttt ccctgttaac gcccgatggc
     5761 gtaacgacgt tttccagctt acagcccggc ggtgaatcct ggagtgtatt acgtgcacga
     5821 ttcgatccgt ggctggttgc cgaagccgaa aaagaaggtg tcgaatgcat ccccggagcg
     5881 acggtggatg cactgtatga agaaaacggc agagtctgtg gcgttatttg tggtgacgat
     5941 attctccgcg cccgttatgt ggtgctggca gaaggtgcca acagcgtcct ggctgaacgt
     6001 cacgggttag tgactcgtcc tgctggcgaa gcgatggcgt tggggatcaa agaagtgctg
     6061 tcgctggaaa catccgctat tgaagaacgt tttcatctgg agaataacga aggcgcagcg
     6121 ttgctgttca gcggcaggat ctgtgatgac ttacccggcg gcgcatttct ttatactaat
     6181 caacaaacgc tctcgttagg gattgtttgc ccgctctctt cccttacgca aagtcgtgtt
     6241 ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg cagtgcgccc gcttatcaaa
     6301 aacacggaat cactggagta tggtgcgcat ctggtgccag aaggtggctt gcacagtatg
     6361 ccggtgcaat acgccggtaa cggctggctg ctggtgggcg atgcgttgcg cagttgcgtc
     6421 aataccggaa tttccgtgcg cggcatggat atggcgctga ctggcgcgca ggcggcggca
     6481 caaacgctga taagcgcctg ccagcaccgc gagccgcaaa atctgtttcc gctttatcat
     6541 cacaacgtag agcgcagcct gctgtgggat gttctacagc gttatcagca tgttccggcg
     6601 cttttgcaac gcccgggatg gtaccgtacg tggcctgcgt taatgcagga tatttcccgc
     6661 gatttatggg atcagggtga taaacctgtt ccaccgctgc gccagttatt ctggcatcat
     6721 ttacgtcgtc acggcctgtg gcatctggcg ggcgatgtta tcaggagtct gcgatgtctg
     6781 tagcccgtaa tctctggcgc gttgctgatg cgccgcacat tgttccggct gactccgttg
     6841 agcgccagac ggcagaacgg ttgattaacg cctgtccggc aggtcttttt tcgctcacac
     6901 cggaaggtaa cttacgtatt gactatcgca gttgcctgga gtgtggcacc tgccgtttgc
     6961 tgtgcgacga atcaacacta caacagtggc gctatccgcc ttccggattc ggcatcacct
     7021 accgctttgg ataaataata aggataattt atgcccctct tacacctgct ccgccagaat
     7081 ccggtgattg ctgccgttaa agataatgcc agcctgcaac tggcaatcga ttctgaatgc
     7141 caatttattt ccgtgttgta cggcaatatc tgcaccatca gtaatatcgt taaaaagatt
     7201 aagaacgccg ggaaatatgc ttttattcat gttgatttgc tggaaggcgc gtcaaataaa
     7261 gaagtcgtta ttcagttttt gaaactggtg accgaggcgg acggcattat cagtaccaaa
     7321 gcctcaatgt tgaaagcggc aagagcggaa ggttttttct gtattcatcg cctgtttatt
     7381 gttgattcaa tttcgtttca caacattgat aagcaagttg cgcaatcgaa tccggattgt
     7441 attgagatcc tgccaggctg tatgcccaaa gtgctgggct gggtgacaga gaaaatccgc
     7501 caaccgctga ttgccggtgg gctggtgtgc gatgaagaag atgcgcgtaa tgcgattaac
     7561 gcgggtgtcg tggcgctttc caccacgaat accggggtct ggacgttagc gaaaaaatta
     7621 ctttgacggg ataatcgtaa ccaattgaat ttggtttgat ttgtaggccg cacgccacat
     7681 ccgacattca gcgcctgatg cgacgcttga cgcgtcttat caggcctaca agtccccagg
     7741 ccagaaccgt aggcaggatc atccggcaac acgttattgg caatctgcgt gaatgtttgt
     7801 caccagtgcc tccagcacca ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa
     7861 taccgcagcg ctggcgtcat gattaatcgc caccacaaac ttgctattgc gtacgcccgc
     7921 cattaacgct gctgcgccag aagcgccaac aacaatacac acctcaggtg ccagcagatg
     7981 tccggaaata ccaatcactt tttcagcatc aacgccaccg ttcatcactc gcgcccgact
     8041 gtagcccact tcggctccca gcttttctgc cagcatagcg atttcctggt tatctgcctc
     8101 tccgccctgc cccaccacca gcacccgtct ggcctcagct aatgggtcgc gggtaacatt
     8161 tttcagatcc tctgtgctaa ccagccagtc cggaagcgca cccgggacaa tgttcagttg
     8221 ttgcattcca gagggcaacg tggcgttttt cgctgctccc gcctgacgcg ccagggaaag
     8281 gcatagcggg cgtttttctg tttgcagcgt cgccgttagc gcatttcccc agtgggattt
     8341 gcgcacgctt acggttggaa tatctaacga agtcacctgg cagatgcttg caccatgtaa
     8401 acgccaggcc agccgggtcg acagttcatc gccaaacgtt cccggcggaa agagaacaac
     8461 gtccgcaggc gttcgctgcc actgttccac caacgcatcc agcacctgtt ccgccactac
     8521 aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg ctgaaatcct gcgccgccag
     8581 ccagcttgct attgcggcat tctcctgatt tattgtcaca attgcgatat tcatggctgc
     8641 atcctttggc gtaaatagtc ctgccatagc ttttgagctt tttctgctac cgtttgccca
     8701 tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca gttgcagaca ctgcatcgct
     8761 ggcatttcgg cagcaacagt tttacggata atttctgctt tccccgcagc cattcgctgg
     8821 cgcattcccg gtactggcaa ggccacttca ccgcactgac gcacggcaat caccgcaggc
     8881 aagcgaaccc ggcaacaacg caacccatgt tcagtacgct gttcgagggt gataaacagc
     8941 gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc agcccagcat ttccgccagc
     9001 aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc agccggtaat tatcagatcc
     9061 agcggattct gatgctgcca ttcggcaata tggcgagcga caaattccgg cgcaaagcgc
     9121 agatctgccg ccgtctccag caatacagct tcctcaaacc caagagccat gagatagcgc
     9181 agccagtgca atgcccgttc atcccccatg ctcaacgcgg ttaaagacat cggcgtaccg
     9241 ttttttctct gcgccaacag cagcgcggca gcggcctgtt catcagcacc gagtaaactt
     9301 cgcagtagcg aaatatccgg tccgctttta ccctgagccg ccgcctgcca ctctttttcc
     9361 gccagcattc cggcatccgg ttcggcttta aacgctaaca gaatgttcat tcgcacctcc
     9421 tacatttccc gccgccacca gcgggagggc tttagtttct ggtgcccata gccaggtcac
     9481 aacaaaacca accaacaaca ctgtcgccag gagcaataag gtgacttgca ttccccactg
     9541 cgccagcacc cacggcagca ggccagtact taccgccgcg ccaagtcgac tcatggcagt
     9601 ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc
     9661 caccagatta ctgactgccg aaatggtggt gctgaagaga acaaaaagca gcagcgttaa
     9721 tgaactgccg gaaggcaaac aggccatgac taccagcgtt gccgccagca gcaaaaaact
     9781 tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc
     9841 cacaattaac aacgcattaa gcatcaggct ggcagtcagc gcatcttcca gaccaatagt
     9901 ctgggcgata gttggcagcc aggtataaat cacaaaccat gggattacga ggcagacaaa
     9961 gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa gagaacaagg ttttgatgtg
    10021 tttatgggtc gccgttacca cttcatcgcc cagtaaaaca tggggaccaa aatagcgatg
    10081 cacgatagcg tgagcttctg caaaacgccc ctggcgcagt agccagcgtg gtgattctgg
    10141 cgttccccag cgtaataacg taatcaacaa cgcgggcaga gctgccgatg ccagtagcca
    10201 gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg gcaatacttg ccagcacata
    10261 gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata ccgcgatggc ggcgcgggga
    10321 aaattcagcc agcaaggtgt gacctactga ataatcgcct cccagaccaa tgccaatcaa
    10381 aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa aattgtaaaa acgaagcaag
    10441 cgtaatcagc aaaaagctga aggtgaagat tttttgccga ccaatatggt cggagatcca
    10501 cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc gagccgccga tcattcccgc
    10561 cataaacggc gtcagttgca tggcgggcgt aagctgaata atggcgtaac caatgacgcc
    10621 gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaatgc ggcagtgaaa
    10681 acggttaagc ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta ccccttcatt
    10741 gtccggtaat aagaacggct tctcgcctga gaagccgaaa aggcaacgcc ccgtcatcgc
    10801 ggaacgctgc ctgacacatt atttttcaat cggatagata gtacccgtat tcataatgcc
    10861 attaggatcg aactgctttt tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag
    10921 tttgctccag tgaacgcgat gtttaccgat accgtggtgg tgcaccatcg aaccgccgag
    10981 gcgaatggtt tcttcacaga tgatcttgtt gagcggattg tggtacttgt cgatttcctc
    11041 ttccggctta cagtcaacga cgttgtaatc gtagacgaag tacatgttgg tgccgttctg
    11101 atagctatga gaggaatgac cgcccagcat ggtgatgtcg tcggcgtgcg ggaactcagt
    11161 acgaatacgg ttaataacgc tttcgtagat ttcgtggatg cagctccagc agccggatac
    11221 ttcggtggta aagcccatgt tgccggtttt gaggatctgc acacgttcgg cagccacttt
    11281 atccggtccc cagttcaggt tgttaaacca ggtttcgatc agcttgctgt ccacgcgctg
    11341 gcattgcggg tagcgggcaa cgatttccgc aatcccttcg cccgtcacct tcgcaatgcg
    11401 agggttacct tcagccataa agatcagcac gcattttccg tcggcaaaat gggtgaagtg
    11461 ttgggtgcca tcttcagcgt catacaaacg agcgattgac ggacgatacc cttccaccat
    11521 gatttcacgc aggatgttga agccggtttt catgtcttcc aggatatagc cgtagaagag
    11581 gttgttttcc ggggtgaatt taaagatttt cactgttact tcagtgatat agcacaatgc
    11641 accttcgttg ccgatgatga tgtgacgaat gtccgggcca gccgcgcggc gtggcacgtt
    11701 tttaatgcgt gtgacggtgc catcagccaa tactgcttcc agaccaacga ccatatcttc
    11761 gattgcgccg tagagtgtgg agaactgccc gatacttcgg gttgctacca ggccgcccat
    11821 ctgcgccagc ggctttgact gcggagaatg ccccgtggtg taaccttttt cacgcaacgc
    11881 gttttccagc acttccagcg gaacaccaca ttgcgccgtc gcctgcatat tctcaatatc
    11941 aatattaatg atttgattca tggcggagcc gtcgagcacc accgagtttt ctacaacagt
    12001 ttccagccca ccttcggtgg cggaagcacc ggtacgcggc acaccgttaa ttttgtgcgc
    12061 attcataaaa ttcagcacac gggatacttg ctctgtggaa ccgagtttta cgaccgctgc
    12121 cggaatcggc aaagtataaa tgccatgaat atccggaaat ttacgaaaac ggtcaatact
    12181 gtttttcttt aatactgttt catcggtaat tacgcgatct gcaccaacaa tttcctttag
    12241 ctggtcgaca atcgctgcgc gagataaaga catagtaatt ccttcctgat aataaaaatg
    12301 gaataatcat taataagaga aaacagaaac caccgttatg ggttagcgca ctaaataacc
    12361 gccatcaacc actaataaat gcccgttgac ataattcgat gccggacttg cgaggaatac
    12421 ggctgcgccc attaaatcct gagtatcgcc ccaacggttt gccggaatat gatcaagaac
    12481 gcgctgattg gtttctggat tactgcgtgt cgccagcgta atatctgttg cataatagcc
    12541 aggggcgata ccatttacct gaatattata ttgacctagt tcatcacaat aagctttggt
    12601 gaacccggca agagcatgtt tagtggcaga atatgcaggt gaccattgtc cacctaagta
    12661 agagaacaat gaacagatat taataatttt gccgcttttc tgcgggatca taatttttgc
    12721 agcttcatag cttaactcga atgcggcggt caggttcaca tcaatcatcg gatcccagtc
    12781 ggcacgaccg aagtccagca ccttattcag cttacaaata ccggcattgt taaccagaat
    12841 atcaactgta ccgaaacgct cacagcaagc agcgataatc ttctgcggcg cgccttctgc
    12901 ggtgatatcc acctgcatga agtccacctc aacaccctgt ttttcaatca tttcctttgt
    12961 ttcgccgtta tctttgacga aactaggaat aaagatattt gcgccagctt tggccaacgc
    13021 catggcaaat gcctggccta aaccgctatt cccaccggta acaattgcgg ttttaccttt
    13081 cagggagaaa aaatccattg agaacgcatt gagagattcg attgacatag ttagctccaa
    13141 tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct
    13201 cattatctct ggtaactgga gataattttt agcataaggg cataactgct aatgtgatcg
    13261 taatcacagt gtgatattcg tattgtcaaa taattttaaa aagcagaagc gtgattactc
    13321 tcacacaaca cctctgcttt ccatgttatt tattatttca attactagag acctgagaaa
    13381 agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta caaatgggaa
    13441 tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat tagttttagc
    13501 ggcggcgtta gtttcgacct ggcttattta cgttatattt atcaaattcc catggcgaaa
    13561 tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg tattgcggcc
    13621 attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg caaaatgatt
    13681 acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta tccaccgctg
    13741 tgggtaatgc tctgtattca gatcgccttt gcgataacga cgattttaat gctgtggtcg
    13801 gtgtcgatta aagctgcatc gttgcttggc gatcatagcg agcaagggaa aattatgggc
    13861 tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc
    13921 ttttctcgct ttgcaccgga tgacagcacc agcctgaaaa cggtcattat catctatagt
    13981 gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa caacaacctg
    14041 cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct ggccgttttg
    14101 cgtatcagca ccacctggta ttgcagcatg gtgatttttg gcgtcttcac catctacgcc
    14161 attctgagtt actccaccaa ctatctgacc gaaatgtatg gcatgtcgct ggtggcggcg
    14221 agctacatgg ggattgtgat caacaaaata ttccgcgcgc tgtgcggccc acttggcggc
    14281 ataatcacca cctacagcaa agtgaaatcc cctacccgcg tgatccaaat cctttccgta
    14341 ctcggcctgc tgacgttaac tgccctgctc gtcacgaact ctaacccgca atcggtcgcg
    14401 atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg cgggctgtac
    14461 tgggcctgcc ctggcgaagc gagaacaccg tcttacatta tgggcaccac ggtaggtatt
    14521 tgttcggtga ttggattcct gccggatgtc ttcgtttacc caattatcgg ccactggcaa
    14581 gacaccctgc ccgctgcaga agcctaccgc aatatgtggc tgatgggcat ggcggcgctt
    14641 ggcatggtga ttgtctttac ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc
    14701 cccgcaatgg ctagcagcaa gtaagccagg aaaattacag gagaaaaatc atgtcgaaaa
    14761 aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgatg tacgatctgg
    14821 aaggtaacgt ggtttgcgaa ggcaaaggct tattacagcc gatgcacacg ccagatgccg
    14881 atactgcaga acatcctgac gacgatttat gggcatcatt atgttttgcc ggtcacgatt
    14941 tgatgagtca gtttgccggg aataaagaag atattgtcgg tattggtctg ggatccatcc
    15001 gttgctgccg tgcgttattg aaagccgatg gcacgcctgc tgcgccgttg attagctggc
    15061 aggatgcacg cgttacacgc ccttacgaac acactaaccc tgacgtggca tatgtcacct
    15121 ctttttcggg ttatctgacg catcgcttaa ccggcgagtt taaagacaat atcgccaact
    15181 attttggtca gtggccggtg gattataaga gctgggcatg gagcgaagat gctgcggtaa
    15241 tggataagtt taatatcccc cgtcatatgc tgtttgatgt gcaaatgcct ggcaccgtcc
    15301 tcggacatat cacaccacaa gccgcactgg cgacacattt cccggcagga ctgccggttg
    15361 tttgtaccac cagtgataaa ccggtagaag ctctgggggc cggattactg gatgatgaaa
    15421 cggcggtaat ttctcttggc acttacatcg cattgatgat gaacggcaaa gcactgccga
    15481 aagatccagt agcgtactgg ccgattatgt cttctattcc gcaaacattg ctgtatgaag
    15541 gttacggtat tcgcaaaggc atgtggacgg tgagctggct gcgcgacatg ctaggcgagt
    15601 cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc aacaaaaaag
    15661 cttcttgtgt gccgccaggc tgtaatggtc tgatgacggt gctggactgg ctgaccaatc
    15721 cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg gattacgcat
    15781 ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct gaagaacaat tacgacaata
    15841 tgtgtaatga aatgaatcac tttgcgaagc atgtgatcat tactggcggc ggttcgaaca
    15901 gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacga cgtaacgcca
    15961 ttaacggttg tgcaagcctg ggggcagcga ttaatacagc ggtaggtctg gggctatacc
    16021 cggattacgc aacggctgtc gataacatgg ttcgcgtgaa agatatcttt ataccgattg
    16081 agagcaatgc caaacgctac gacgcgatga ataaaggcat tttcaaagac ctaaccaaac
    16141 atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg gggaatgtgg
    16201 attcgatcca gagctggtcg aatgcgtaag tgaggaaggc cgggcgggaa actgcccggc
    16261 ctgaacatac ctgaatggtt atccccgctg acgcggggaa cataagttcg acaacttaaa
    16321 aaatgataac aagtccggtt tatccccgct gatgcaggga acataataaa aactttaact
    16381 agttcggttt atccctgttt ttacaaggaa tattaactac actcgatgtc tttagaataa
    16441 aagacaatac gaatcgcact ttatcaattt tcacttaaag tatgaactgg atatctttat
    16501 tcataagaac tattcatcaa cagcgtatag aggcggttat gtaaaaccac tcattagcct
    16561 caaaacaaag acaaacttcc agatttatta cttaacc
//
LOCUS       DBBGLQ010000094        16589 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.094, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000094 DBBGLQ010000000
VERSION     DBBGLQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.094"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..680)
                     /locus_tag="ACIYSB_004046"
     CDS             complement(<1..680)
                     /locus_tag="ACIYSB_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430980.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLAQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVIANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFV"
     gene            complement(1309..1734)
                     /gene="arsC"
                     /locus_tag="ACIYSB_004047"
     CDS             complement(1309..1734)
                     /gene="arsC"
                     /locus_tag="ACIYSB_004047"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGC5430981.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1747..3036)
                     /gene="arsB"
                     /locus_tag="ACIYSB_004048"
     CDS             complement(1747..3036)
                     /gene="arsB"
                     /locus_tag="ACIYSB_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGC5430982.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3090..3443)
                     /gene="arsR"
                     /locus_tag="ACIYSB_004049"
     CDS             complement(3090..3443)
                     /gene="arsR"
                     /locus_tag="ACIYSB_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGC5430983.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4183..4266
                     /gene="dinQ"
                     /locus_tag="ACIYSB_004050"
     CDS             4183..4266
                     /gene="dinQ"
                     /locus_tag="ACIYSB_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGC5430984.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4320..5672)
                     /gene="gorA"
                     /locus_tag="ACIYSB_004051"
     CDS             complement(4320..5672)
                     /gene="gorA"
                     /locus_tag="ACIYSB_004051"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGC5430985.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5744..6586)
                     /gene="rlmJ"
                     /locus_tag="ACIYSB_004052"
     CDS             complement(5744..6586)
                     /gene="rlmJ"
                     /locus_tag="ACIYSB_004052"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGC5430986.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARLLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGIYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6789..8831
                     /gene="prlC"
                     /locus_tag="ACIYSB_004053"
     CDS             6789..8831
                     /gene="prlC"
                     /locus_tag="ACIYSB_004053"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGC5430987.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8839..9591
                     /gene="rsmJ"
                     /locus_tag="ACIYSB_004054"
     CDS             8839..9591
                     /gene="rsmJ"
                     /locus_tag="ACIYSB_004054"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGC5430988.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9640..11109)
                     /gene="dtpB"
                     /locus_tag="ACIYSB_004055"
     CDS             complement(9640..11109)
                     /gene="dtpB"
                     /locus_tag="ACIYSB_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGC5430989.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11275..11328
                     /locus_tag="ACIYSB_004056"
     CDS             11275..11328
                     /locus_tag="ACIYSB_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430990.1"
                     /translation="MVLNVVLAGDFDCVRRP"
     gene            11306..11359
                     /locus_tag="ACIYSB_004057"
     CDS             11306..11359
                     /locus_tag="ACIYSB_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5430991.1"
                     /translation="MIAYADRRPDKVFTLIR"
     gene            complement(11426..11860)
                     /gene="uspA"
                     /locus_tag="ACIYSB_004058"
     CDS             complement(11426..11860)
                     /gene="uspA"
                     /locus_tag="ACIYSB_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGC5430992.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKGISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12251..12586
                     /gene="uspB"
                     /locus_tag="ACIYSB_004059"
     CDS             12251..12586
                     /gene="uspB"
                     /locus_tag="ACIYSB_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGC5430993.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12830..14329)
                     /gene="pitA"
                     /locus_tag="ACIYSB_004060"
     CDS             complement(12830..14329)
                     /gene="pitA"
                     /locus_tag="ACIYSB_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGC5430994.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14561..15763
                     /gene="yhiN"
                     /locus_tag="ACIYSB_004061"
     CDS             14561..15763
                     /gene="yhiN"
                     /locus_tag="ACIYSB_004061"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGC5430995.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16078..>16589)
                     /locus_tag="ACIYSB_004062"
     CDS             complement(16078..>16589)
                     /locus_tag="ACIYSB_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HGC5430996.1"
                     /translation="DITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTFSTKEHWL
                     WCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWLLALVWRR
                     TCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCFTLFSIVS
                     ILEAGSAKK"
BASE COUNT         4243 a         4124 c         4253 g         3969 t
ORIGIN      
        1 acgaacccgg acaggttctg tcctgtcata ttgaaatcaa taaagttggt tccggcaaag
       61 actttcatcc tgtttttatt ttcactgtgc tcaatatata acttcgtcat tttttgatac
      121 aggctttgaa tatcttttat attagttggt aagtcagctc gttgttcagc aataaaatta
      181 gtatctgaaa acatggtttg gacactgaat gttatgggta ttccctcttc tacgctaatt
      241 ttagttatga ttgccttgtc gggtttagga ttagcgataa caatgattcg acattcagca
      301 cttttgtttt tttcatatct aatgttatta atctgattaa gtattgattg tgtctgttgc
      361 gtaagttgtg tcgaacttat cgtttcaatg ttagtaataa gacttacaag ttgagccaca
      421 acttttgagt agtctgcatt tgtatatgta gtgttcgtgt ggcacgcatt aatttgttga
      481 agttggtgtt gcgctaaatc atattgatca gagatatatc cttctccaga gaatgtggaa
      541 ttaatgggtt gttgttcttc ccaattatcg caaggccact tcggtgtgtc tggcgtttta
      601 gcattatcgg tatttgttgt gcagttataa atatcgccgt gatatgtatt tattatacct
      661 ggggtaaagt caattgacat aaaattcctt attcatcatt tgttttatgc taagatgttt
      721 aaattggata attagaacag gaatggattt tcttctacac acaataaatg taatgcgtta
      781 tatcttcctg atataattac ctttgatttc ccttttatct ccacccggta atatttgtgt
      841 gtaaaaaaag ggtagtgata gtatcgtaaa ggaaatcgtt aacaactatc aaaatgcgct
      901 cattttaaaa agttattatg ttctctgata gtgtgtgaag tgattttttt tgtgatgttt
      961 tagatgtgta ataataaaat gagttaaggt atcttataat tatttaataa tttgttgtgt
     1021 aaaccactga taaatcataa tccagtacgt ctttaaacta ctgcgctgta tcgctgataa
     1081 ctaagcagct cactgataca ccatgctgga cgcgataatt ggaatctcac gcagggagta
     1141 ctgtgtaaat agcaaaatcg cctgatgcgc tacgcctgtc aggcctacaa ataacatact
     1201 attcatcgaa ttttcataac tttgtaggtc ggataagacg cgccagcgtc gcatccgaca
     1261 taaataaagc gcacttttct aacaacctgt gggggggata tcgccgcttt atttcaggcg
     1321 cttacccgct tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc
     1381 atctggcaga atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt
     1441 caccacaatc gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc
     1501 gtcagtaaat ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag
     1561 cagcgcgcgt acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg
     1621 cggagtttcc agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag
     1681 cgtattacgc gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt
     1741 atctcattac aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac
     1801 aggcagggtc atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt
     1861 ctgcgaaagt acgtgcagcc agagtagcgt agccaggcta ccaattgggg taattttcgg
     1921 tcccaaatcg cagccaatca cattggcata aaccatcgct tctttgataa cgccagatgc
     1981 cgtgctgcca tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga
     2041 agagaggaag gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc
     2101 cagcacgttg agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac
     2161 caccagatac atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt
     2221 acccgtatta atcgcatgac cgcgtttagc gacgacaaat aatatcagcg cgcccacagc
     2281 tgcaatggcg ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag
     2341 cagtaaaaca acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga
     2401 tttcagcagc gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtaacat
     2461 caccagcgtg gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg
     2521 aaagccgagg ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg
     2581 caggctggcg gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tagtgccttt
     2641 actgaaccct aaagccagca gcatggcgat gacaatcggt gtcaaaataa gcgccgcgcc
     2701 atcattggca aacagggcgg caacggcagc accgagcagg acaatccagg taaacagcaa
     2761 gcgaccacga ccattacccc agcgtgagac gtgcagcgcc gcccattcaa aaaagccgga
     2821 ctcatccagc agcaggctga tgataatgac ggcgataaac gcagccgtcg cgttccagac
     2881 gatattccac accaccggaa tatcacccgg atggaccacg cccgtaacta acgccagtac
     2941 tgcgccgagc gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac
     3001 gatggtcagg acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatc
     3061 tgaaaattca tatgtgttta gctaaatttt taactgcaaa tgttcttact gtccccggaa
     3121 cagttttgtc gagccaggtt gcggacaatc gcctgaacct tttcctgttc acatcgccag
     3181 gcctcatcaa taattttcgc cgcccatgct ggaatatgcg gtgataagcg gtaatgaacc
     3241 cacttacctt gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc caggtggcgg
     3301 gagatcttgg gctgcgactg gtcgagagca gtgcagagat cgcagacgca taactctccc
     3361 agttcgctga gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat
     3421 tggatgggta acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc
     3481 gaagcggata agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa
     3541 taggtgtgaa ttttgagttg gctattcatt tgaaaggagg tctgaatcag gacgtaaaaa
     3601 aaccgacttt gcgtcggttt tttactttcc agccctgagt tggtggctct ggctggagtg
     3661 agagagttct tatctaacag ctcaatacta ttagccagtg gctaacattg caagaattag
     3721 cgatttcttc agctggcgga gggagcactt cggtatatat cgcagtgacg atttatatac
     3781 tttcactggg tcatcgtcat attaagcctt gtccgaatcc ggatgtaaaa aaaccgactt
     3841 tgcgtcggtt ttttactttc cagccctgag ttggtggctc tgactggagt gagatagcca
     3901 tcatctaacg catcaatgct aataccatac tgaaactatt gcaaggacgt gctggtttta
     3961 taacctgcat gtactgtatg attatccagt tagctctgag gcattttcac tctggcaatg
     4021 cgcataaacg ctttcaaagt cctggtcaga agtacgggtg gtgccgttaa ctgatgctct
     4081 ggccggagtg agagagttct tatctaacaa tgagacatgc gccgtgacag gcagtggatg
     4141 agtaagcgga tgcattctca ctccatcgca tggagaaaac gggtgattga taaagcaatc
     4201 atcgttctag gggcgttaat tgcgctgctg gaactgatcc gctttctgct tcagcttctg
     4261 aactgatagc ggaaacgtaa ttaagggcta agagcacact actcttagcc ctttaacatt
     4321 taacgcattg tcacgaactc ttctgccgcc gttgggtgaa tggcgacggt attgtcgaag
     4381 tcttttttgg ttgcccccat cttcagcgcc accgcgaagc cctgcaacat ttcgtccata
     4441 ccaaagccaa tgccgtgaat accgacaatc ttctcttccg atccaacgca caccagcttc
     4501 atgcggcacg gctggcggtg agtggtgacg gcggtataca tcgcggtgaa agaggattta
     4561 tacactttca cctgatcgtc gccatactgc tcgcgcgcct gcggttccgt taaaccaaca
     4621 gtaccaatcg gcggatggct gaagaccacg gtcggaatgt tgctgtaatc cagatgctca
     4681 tccggcttgt tattaaacag gcgttcagag agacggcgac ccgctgcaac tgccaccggt
     4741 gtcagctcca ctgcacccgt gttatcgccc accgcgtaaa taccttcaat attggtgttt
     4801 tgatatttat cgacgacgat atagcctttt tcgttagttt taacgccagc ggcttccagg
     4861 ttgatgttgt cattggcagg ctcgcgacca atcgcccaaa tcaggcaatc caccgtttca
     4921 ctgcgaccat cttccagctc cagcgtcagg ctaccatcgg catttttcac taccgctttc
     4981 gggatggcgt tggtgtgcag ctgcgggcct tcggcgttca tcacttcgac cagcgtttcg
     5041 gaaatcatcg ggtcgaagct gcgcagcggc gcatgtttac gcacaaacag atgcgttttc
     5101 gcgccgaggc cgttaatcac gcccgccagc tcaacggcga tgtaacccgc gccaacaacc
     5161 gccacgcgct ctggcaaagc aggaagggcg aagaagccat cagaatcaat accgtattcc
     5221 acgcccggaa tatccgggtg gctcggacga ccgcctgtgg cgatcagaat atgatcggcc
     5281 gtgatggttt cgccgtttac ctccagcgtt ttggcatcaa cgaagcgggc aaagcctttg
     5341 attacatcaa cgttattttt accgagcacg ttttcatagg aagtatgaat acggtcgata
     5401 taggcggtac ggctggcgat caacgtttcc cagttgaatt tattgatagt ggtatcaaaa
     5461 ccataatccg ggccgtacat atggatcgct tcacggattt gcgccgcgtg ccacatcact
     5521 tttttcggca cacagccaac atttacgcag gtgccgccca gctctttggc ttcaatcagc
     5581 gcacatttct ggccgtacat agccgcgcgg ttgatggagg cgataccgcc gctgccgccg
     5641 ccgatggcga tgtaatcata gtgtttagtc atgacaaagt gtccttatcg ttgattaccg
     5701 cgattgtagc gcgagacgta ataggtgcca gcaatggctg caattactcc ggcacgatcc
     5761 agcttacggt ggcgtgcccg gtgcctgccg gaaccagttt gctgtgcagc cacggcagca
     5821 cgttattcat ctgttgttcc agtttccacg gcgggttaat cacaatcatg ccggaagcgg
     5881 tcatgccacg gcgatcgctg tctggcagta ccgccagttc aatttgcaga attttgcgaa
     5941 taccggtcgc ttccagatcg tggatcatgc gcttaatttg ctgacgcagc accaccggat
     6001 accacagtgc gtaaatacca gtggcgaaac gtttgtaacc ttctgctatc ccgctgacca
     6061 ccgcttgata gtcagttttc atttcatacg gcgggtcgat aaggattaaa ccacggcggg
     6121 aaaccggcgg cagtttggcc ttaagctgct ggaaaccgtc ggctttttcg acacgcgcac
     6181 ggctatcttt ctgaaattca gaacgcaaca acgggtaatc gctcgggtgc agttcggtca
     6241 gttgcaggct gtcctgttca cgcagtagca ggcgagcaat caacggcgaa ccggggtagt
     6301 aacgcaactg accgctacgg ttgaagtgtt ttaccacatt gatgtacgcc tccagttctg
     6361 cgggcaaatc gtcctgctgc cagatacggg cgatgccttc gagatattcg ccggtacgct
     6421 cggcatgttc gctgcctaac tgataacgcc cggcccctgc gtgggtgtcg agatagagaa
     6481 acggtttatc tttctctttc agcgactcga tgatcaggct ctgaacggta tgtttaagga
     6541 cgtcggcgtg gttgccagcg tgaaagctgt ggcgataact gagcatgggt aaaggtgttc
     6601 cggtaagtaa aaatcggccc gtattcgggg cgcacaaaag cgtatcagga cagtataccg
     6661 aaaagaggcc gccgccgcga aagcgtaacg tttctcattg aaattcacta cacttaaccc
     6721 catgctacac acattatgta aagcgcctgt tgagcgcttc cttaacctct ttaaccagga
     6781 ctgcgcgaat gacgaatccg ttactgactc cctttgaatt gcctccgttt tctaaaattc
     6841 tcccggaaca tgtcgttcca gccgtgacta aggcattgaa cgactgccgc gaaaacgtgg
     6901 agcgcgtagt agcgcaaggg gcaccgtaca cctgggaaaa tctctgccag ccgctggcgg
     6961 aagtggacga tgtgttgggg cgtatcttct ccccggtcag ccacctgaac tcggtgaaaa
     7021 atagcccgga actgcgtgaa gcctacgaac aaaccctgcc gctgctgtcg gaatacagca
     7081 cctgggtagg gcaacatgaa gggctgtata aagcgtatcg cgacctgcgc gatggcgatc
     7141 attacgccac gctgaacacg gcgcagaaga aagcggttga taacgcactg cgcgactttg
     7201 aactctctgg cataggtctg ccgaaagaga aacagcagcg ttacggcgaa attgcgaccc
     7261 gtctttctga actgggcaac cagtacagca acaacgtcct cgatgcgaca atgggctgga
     7321 ccaaactcgt taccgacgaa gcggagctgg cggggatgcc agaaagcgcg ctggctgcgg
     7381 caaaagccca ggccgaagcg aaagagctgg aaggttattt gctgacgctg gatatcccaa
     7441 gctacttgcc ggtaatgacc tactgcgaca accaggctct gcgtgaagag atgtatcgcg
     7501 cttacagcac ccgcgcctcc gatcaaggcc caaacgccgg taaatgggat aacagcaagg
     7561 tgatggaaga gatcctcgcg ctgcgtcacg aactggcgca actgctgggc tttgaaaact
     7621 acgcctttaa atcccttgcc actaaaatgg cagaaaaccc gcagcaggtg ctggatttct
     7681 taaccgatct ggcaaaacgc gcgcgtccac aaggcgaaaa agagctggcg caattgcgtg
     7741 cctttgccaa agccgaattt ggcgtcgatg agttgcagcc gtgggatatc gcttactaca
     7801 gcgaaaaaca aaaacagcac ctctacagca tcagtgacga acagctgcgt ccgtacttcc
     7861 cggaaaacaa agcggttaac ggcctgtttg aagtggttaa gcgtatttac ggcatcaccg
     7921 ctaaagagcg taaagatgtt gatgtctggc atccggatgt acgtttcttc gaactgtatg
     7981 acgaaaataa cgaactgcgc ggtagcttct acctcgatct gtatgcccgt gaaaacaagc
     8041 gcggcggggc gtggatggat gactgcgtag gccagatgcg taaagctgat ggttctttgc
     8101 aaaaaccggt cgcgtatttg acttgtaact tcaaccgccc ggtaaatggt aaaccggcgc
     8161 tgttcactca cgacgaagtg atcaccctct tccacgagtt cggtcacggc ctgcaccata
     8221 tgctgacccg catcgaaacc gctggtgttt ccggtatcag cggtgtgccg tgggatgcgg
     8281 tcgaactgcc gagtcagttt atggaaaact ggtgctggga gccggaggcg ctggcgttta
     8341 tctctggtca ctatgaaacc ggcgaaccgc tgccgaaaga gttgctggat aaaatgctgg
     8401 cggcgaagaa ctaccaggcg gcgctgttta ttctgcgtca gctggagttc ggcctgtttg
     8461 atttccgcct tcatgccgag ttccgcccgg atcagggggc aaaaatcctc gaaactctgg
     8521 cagaaatcaa gaaactggtt gccgtggtgc catctccgtc ctggggccgt ttcccgcacg
     8581 ctttcagcca tattttcgcc ggtggttatg ccgcaggtta ctacagctac ctgtgggctg
     8641 acgtactggc ggcagatgct ttctcgcgct ttgaggaaga gggcattttc aaccgtgaaa
     8701 ccgggcagtc gttcctcgac aacattctga gccgtggcgg ttcagaagag ccgatggatc
     8761 tgttcaaacg cttccgtggt cgtgaaccgc agctggatgc gatgctggag cattacggca
     8821 ttaagggctg atcattcagt gaaaatctgc ttaattgatg aaacaggcac cggagacggt
     8881 gccttatctg ttctggcggc ccgctggggg ctggagcacg atgaagacaa cctgatggcg
     8941 ctggtgttaa cgccggaaca tctggaattg cgcaagcgtg atgagccaaa acttggcggc
     9001 atctttgttg attttgttgg cggagcgatg gcgcaccgac gcaaattcgg cggtggtcgc
     9061 ggtgaggcgg tggcgaaagc ggtgggcatt aaaggcgatt atttgccgga tgtggtggat
     9121 gccactgcag gacttgggcg cgatgccttt gtactggctt cagtcggctg ccgcgtgcgg
     9181 atgctggagc gtaatccagt ggttgccgcg ctactcgacg acggcctggc gcgtggttat
     9241 gcggatgcgg aaatcggcgg ctggttgcag gagcggttgc agttaattca cgcctccagc
     9301 ctgacggcgc tgactgatat taccccgcgc ccgcaggtgg tttatctcga cccgatgttc
     9361 ccgcataagc agaaaagcgc gctagtgaaa aaagagatgc gtgtctttca gtcgctggtg
     9421 ggaccggatc ttgatgccga tggattactg gagcctgcac gcttgctggc gaccaaacgc
     9481 gtggtggtaa agcgcccgga ctacgcgcca ccgctggcga atgtcgccac gccaaacgcg
     9541 gtagtcacta aagggcatcg ctttgatatc tatgcaggta cgccagtgta aaactaaaac
     9601 agggtagcga taacgctacc ctgctttctg caagaataat taatggcttt ccggcgtcgc
     9661 aatcatgcgt ttcagccacg gcaccatcaa cagcatcact actgcaacgc ccagcgtgac
     9721 cagaccaatc ttaccaaaca cgttggtata gacgggcaac gtctcaagcg gatcggtaat
     9781 gttgtccggc accgcggtaa atgttgccac atagccgccc agcaagaacg cggcagcctg
     9841 cgtcaggaac cacatcccga gaataaagcc catcaaatgc tgcggcacca gggcagcaat
     9901 catcgccagg ccaagggcgc taataaacag ttcacctaag ctctggaata agtacaccag
     9961 cacgataaac catggcgatg tcagcccttg tgcatccgca aaccacattc ccgcagctgc
    10021 cgccgtcaaa aagcccagtg agcacataaa catgccgaga gtaaatttca tcggcatcga
    10081 gaggtctttg cctttgttac ccagatgcgt gtaaatgcct gccagtattg ggctggcgag
    10141 taccacccag aacgggttaa gcgcctggaa gctgaccggg ttgatggaaa aaccgagaat
    10201 ttcatgatgc acgttgttga tggcaaagaa gttcagcgat gttggcatct gggcgtagag
    10261 aatgtaaaac accaccgctt cgagcatcag gacaaaggcg acaaacattt tattgcgccc
    10321 ggttttatcc agcttgaatg cctgacgaaa gaagatgatg gtgacgacga tggagagaac
    10381 aatcagcacc agattggcga cttctacgtt gtgcatcagc catgcgcata cgaagatcat
    10441 caccacgctg ccaagtaaca cgtacaacag tttgctgaag ctcattggcc ggaagtcggg
    10501 ttcagaacca atgtctttca ccattccacg acaggcgatg taaaccagta atgcgataat
    10561 taaccccgcc ccgcacaggt tgtaggtgac tgaataaccg aatctatcag cgatcacagg
    10621 ggccagcgat aacgctatca acgagccgat gttgatcgac atatagaaca gggtgaatgc
    10681 gccatcaagc cgcggatctt tcggcggata gcacttcgaa agcaagctgg ctgggttagc
    10741 tttaaacagg ccgttaccga cagcgatagt ccccagggcg atgaaaatca ggtcaggctt
    10801 aagtagcgac atgccggtca tgaagtagcc aatcgccagc acaagtgctc caagaacaat
    10861 ggtgcgtttg gtccccagca ggtggtcgcc gacatagccg ccaatggaaa tgaggccata
    10921 gaccagcgca gcaaaagcac caaaagtgac aaaagcctgc tcttgcgaga atccaagctg
    10981 tttaacgaag aaaaccgcca gtacgccctg cacgccgtag tagccgaatc gctcccataa
    11041 ctcgacaaaa aagatcatga aaaatgggcg aggttgctgc agcatcccca tgggtgttgt
    11101 tgtattcata tttattaacc ttccaatacc atcctgaaaa gtatgaacgc gtaagggata
    11161 acgcgcgaaa aagtttacat ctgtcagaga ttcttaatat tgatgcggtg aatagattaa
    11221 tacactatta caacagaaaa taaccagatg atcacactaa tgatggaaaa tatgatggtg
    11281 ttaaatgttg tattggctgg ggattttgat tgcgtacgca gaccgtaggc cagataaggt
    11341 gtttacgctg atcaggtaac aaggcgcgcc aaaaagcaaa acgcccgcat atgcgggcgt
    11401 tcaggaacac gtcgtagagg gaagattatt cttcttcgtc gcgcagcgga acaatcagca
    11461 tatcaacgtg aacggtgttg atcagctgac gtgcggaaga catcagtttg ctccagaagt
    11521 cctggtggtg accacaaacc accaaatcca tatcgtattt cttgattgca tcgaccagaa
    11581 cctggcccag gtcgccgctg ccgctcaggg tttcagtgat tgggtagcct gcattagtgg
    11641 aaagctcggt cagtgcatga tgtgtctctt cagagatgcc tttctgcata tcacccagat
    11701 tcacatcaat aagcccggtg tataggtcag agtagtttac atctacgtgg atcagagaaa
    11761 ctttcgcatt gtaggggcga gccatagaga ctgctttctc taccagaact ttgctttccg
    11821 gggagaggtc gaccgcgatg agaatgtgtt tataagccat agtgttactc cttccataaa
    11881 gttgtcgatg actggccagc tagcgtttct tgtgccgtta cgtcacccgc gtcctgcgaa
    11941 ctacgtgcgc ggggctaacc cagccgtaaa gattcagtgt taagaccatc cttaccttat
    12001 agcgacccgg atgatccgtc aatccgcctt gcttactcgt tagataaaca aattttcgac
    12061 atgaatatat tgatagtggt taaccttctg gaaaaaaaac aacctgatct cctacactat
    12121 ctatagagcc gctcgtatgt tggtcaccac aaaagcggtt tctccaggac ctggtgttaa
    12181 ctgattggca gcgtatcggg agcggtagcc ccggggtgga actccgcggg caggtcgccg
    12241 gggaggaggt atgataagca ccgtcgcatt attttgggct ttatgtgtcg tttgcattgt
    12301 taacatggcg cgctatttct catcactacg cgcgttgtta gtggtactgc gtaactgcga
    12361 tccattgctc tatcaatatg ttgatggagg gggctttttt acctcacatg gccaacccaa
    12421 caaacaggtg cgtctcgttt ggtatatcta tgcccaacgt tatcgcgatc atcacgatga
    12481 tgaatttatt cgccgctgtg agcgggtgcg tcggcagttt attctgacca gcgcattgtg
    12541 tggtctggtg gtggtcagcc tgattgcatt gatgatttgg cattaagcac ttcagcaata
    12601 ataacgcata aaaaaagcgg gccagtaaac tgacccgctc gagactgatt acaaacgcaa
    12661 aattgcctga tgcgctacgc ttatcaggcc tacataatct ctgcaatcta ttgaatttgc
    12721 atgtttttgt aggccggata aggcgttcac gccgcatccg gcatgaacaa agcgcactat
    12781 gtcacaatct gaagcgggcc agctgacccg ctcgttttgg tgcgtacgat tacaggaact
    12841 gcaaggagag ccagtacagc ccgccggaaa gcagtaccgc agccggaagg gtaaacaccc
    12901 aggccatcag aatgctggtc acggttttac gctgtaagcc gccaccatct accaccatcg
    12961 tccccgcgac agaagaggag agtacgtgag tagtggaaac cggcatcccg gtataactcg
    13021 ccaggccgat agacactgcc gccgtcatct gggcagacat cccctgagcg taggtcatgc
    13081 ctttcttacc gattttctca ccgatagtcg ttgccacacg gcgccagcca atcatcgtac
    13141 cgatacctaa cgccagcgcg accgccatga tgatccacac cggtgcatac tcgatggtgc
    13201 taagcatgtc ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc acgccaggca
    13261 tcttcaccac tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctggctac
    13321 gttgatcaag cgacagcttg tcgtagcttt ccacatcggt ggtcaacata cctttcaggc
    13381 ggttgagcgc gttaatggta ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc
    13441 cagcttccgg agccggtact aactgatcag caccggtagc ctgtttgagc agcgcaggat
    13501 gctgctcaaa gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc
    13561 cagtggcatt catgttcacc acgaagcctg ctggcgcgac gccaatcaat accaacataa
    13621 ccagaccaat gcctttctga ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg
    13681 aaaggatcag cgcaatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac
    13741 gctccgctgg ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca
    13801 gcaagaaaat cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac
    13861 cgaaaatact taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca
    13921 acgcattggt taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag
    13981 gtaaaccaaa gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca
    14041 ccatggcaag gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca
    14101 caatggcata ggcaacactc agaccaccca gcaaaacacc caaaaagttg aataccgccg
    14161 ccataaccac ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt
    14221 tggctgtgtc atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca
    14281 ataataacag cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg
    14341 ccattttgag gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga
    14401 aatatagact ttttttgatt tgccccctgt atggatttca ctcaaaaaat aattatctta
    14461 tataattcag gcaaatactt ccttttagta atattgatgc tggtgcgacc actgaggaat
    14521 ctttacaatt cacgcccgtt ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat
    14581 tattataggc gctggtgcgg cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg
    14641 ccgggttctg ctgatcgata atggtaaaaa accagggcgc aaaatcctta tgtctggcgg
    14701 tgggcgctgc aactttacca acctttatgt cgaaccaggc gcttatctga gccagaatcc
    14761 gcatttttgt aagtctgcac tcgcacgttt tacccagtgg gatttcattg atctggtcaa
    14821 taaacacggc atcgcctggc acgagaaaac gttagggcaa ctcttctgcg atgactccgc
    14881 gcagcagatt gtcgacatgc tggtggatga gtgcgagaag ggcaatgtga ccttcagatt
    14941 gcgtagcgaa gtgctgagtg tggcgaagga tgaaacaggc ttcacgcttg atctgaacgg
    15001 catgactgtc ggttgcgaaa agctggtcat cgcgactggt gggctgtcaa tgccggggct
    15061 gggcgcgtcg ccgtttggtt ataagattgc cgaacaattt ggcctcaacg tgctgccgac
    15121 ccgcgcgggt ctggtgccat tcactctgca taaaccgttg ctcgaagagt tacaggtgct
    15181 ggcgggcgtg gcggtgcctt ccgtgattac cgctgaaaac ggcaccgttt tccgtgagaa
    15241 cttactcttc acccaccgcg gcttgtctgg accggcggtg ttgcagattt caagctactg
    15301 gcaaccgggg gaatttgtca gcatcaatct gctaccggat gtggacctcg aaaccttcct
    15361 gaatgagcag cgtaacgcac atccgaatca aagcctgaaa aacacactgg cggttcatct
    15421 accgaagcgg ttggttgaac gcttacagca actcgggcaa atcccggatg tttcgctaaa
    15481 acagctcaac gtgcgtgacc aacaggcact gattagcaca ttgaccgact ggcgcgtaca
    15541 acccaacggc actgaaggct atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa
    15601 cgaactctct tcacggacga tggaagcgcg caaagtgcct gggctgtact tcatcggcga
    15661 agtgatggat gtcaccggct ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc
    15721 gtgggcttgt gcgcaggatt tgattgcagc aaagtcgtcc tgacctattt cctaaagatg
    15781 aaaattatta gagtcgcatt aaaaatgagc taattttgat agtggttatc ttgtgattat
    15841 tttctaatga gcccgtgaac tgaaaccctc catgcttaat ataaggtgga tggaaaggtg
    15901 attgaaaact cacccagtgg ccagatcttt atgaaatatg aacaggttga aaaaaacagt
    15961 aactttgctg ttttttatac ttaaccacta tgcattaatg ctgcgttatt tcatgatgcc
    16021 taagaaaaac cagcgttacg caaatggtca acgctggttt tatccggtac gttgcaatta
    16081 ttttttagca gaacccgctt ctaatattga aacgattgag aacaacgtaa agcataccgc
    16141 tcccaaaccg accagaactc gcgaaggaat aaaatatccc atgtcggtct gcgccatttc
    16201 cgcaagaaac gatgccagga aaaggcaaaa caggcaggtg aagacgggaa tcatcggtat
    16261 tctgttggct aacgaacagg tacgtctcca taccagtgcc agtagccaga cttttgagaa
    16321 tatgctgtaa cagatcattc cgaggcaaat aagaataatg ccgggagcca gtcgggcgct
    16381 tgcatcggaa ctgactaaga cgtatatccc ctgcagtacc gtgattgagc cgagaaaaat
    16441 aacccaataa caccacagcc agtgttcttt agttgaaaac gtattacgtg tttgatggac
    16501 aattgtggca acaaggccaa ttagacaggc acagattgcg gttaacccaa gcaatacgtg
    16561 acccgcgaca taatgcggag ttatgtcag
//
LOCUS       DBBGLQ010000095        16223 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.095, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000095 DBBGLQ010000000
VERSION     DBBGLQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.095"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..2138)
                     /gene="rnr"
                     /locus_tag="ACIYSB_004063"
     CDS             complement(<1..2138)
                     /gene="rnr"
                     /locus_tag="ACIYSB_004063"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGC5430997.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPSTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAV"
     gene            complement(2177..2602)
                     /gene="nsrR"
                     /locus_tag="ACIYSB_004064"
     CDS             complement(2177..2602)
                     /gene="nsrR"
                     /locus_tag="ACIYSB_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGC5430998.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(2807..4105)
                     /gene="purA"
                     /locus_tag="ACIYSB_004065"
     CDS             complement(2807..4105)
                     /gene="purA"
                     /locus_tag="ACIYSB_004065"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGC5430999.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4209..4406)
                     /gene="yjeT"
                     /locus_tag="ACIYSB_004066"
     CDS             complement(4209..4406)
                     /gene="yjeT"
                     /locus_tag="ACIYSB_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGC5431000.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4488..5492)
                     /gene="hflC"
                     /locus_tag="ACIYSB_004067"
     CDS             complement(4488..5492)
                     /gene="hflC"
                     /locus_tag="ACIYSB_004067"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGC5431001.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFFGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5495..6754)
                     /gene="hflK"
                     /locus_tag="ACIYSB_004068"
     CDS             complement(5495..6754)
                     /gene="hflK"
                     /locus_tag="ACIYSB_004068"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGC5431002.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(6840..8120)
                     /gene="hflX"
                     /locus_tag="ACIYSB_004069"
     CDS             complement(6840..8120)
                     /gene="hflX"
                     /locus_tag="ACIYSB_004069"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGC5431003.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8196..8504)
                     /gene="hfq"
                     /locus_tag="ACIYSB_004070"
     CDS             complement(8196..8504)
                     /gene="hfq"
                     /locus_tag="ACIYSB_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGC5431004.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8590..9540)
                     /gene="miaA"
                     /locus_tag="ACIYSB_004071"
     CDS             complement(8590..9540)
                     /gene="miaA"
                     /locus_tag="ACIYSB_004071"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGC5431005.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9533..11380)
                     /gene="mutL"
                     /locus_tag="ACIYSB_004072"
     CDS             complement(9533..11380)
                     /gene="mutL"
                     /locus_tag="ACIYSB_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGC5431006.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11390..12727)
                     /gene="amiB"
                     /locus_tag="ACIYSB_004073"
     CDS             complement(11390..12727)
                     /gene="amiB"
                     /locus_tag="ACIYSB_004073"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGC5431007.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(12746..13207)
                     /gene="tsaE"
                     /locus_tag="ACIYSB_004074"
     CDS             complement(12746..13207)
                     /gene="tsaE"
                     /locus_tag="ACIYSB_004074"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGC5431008.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13179..14726)
                     /gene="nnr"
                     /locus_tag="ACIYSB_004075"
     CDS             complement(13179..14726)
                     /gene="nnr"
                     /locus_tag="ACIYSB_004075"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGC5431009.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLYAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            14725..15864
                     /gene="queG"
                     /locus_tag="ACIYSB_004076"
     CDS             14725..15864
                     /gene="queG"
                     /locus_tag="ACIYSB_004076"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGC5431010.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(15847..15900)
                     /locus_tag="ACIYSB_004077"
     CDS             complement(15847..15900)
                     /locus_tag="ACIYSB_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431011.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(16134..16209)
                     /locus_tag="ACIYSB_004078"
     tRNA            complement(16134..16209)
                     /locus_tag="ACIYSB_004078"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16174..16176),aa:Gly,seq:gcc)
BASE COUNT         3704 a         4633 c         4091 g         3795 t
ORIGIN      
        1 accgcttcga cgcgaacttc cacgcgatcg cccaggcgat aagtctggcc gctggattcc
       61 cccatcaggc gttgccctac ctggtcaaag cgatagtagt cattgtccag cgaagagaca
      121 tggaccagac catcaatgaa caagtcgtcc agacggacga agaagccaaa gccagtgacg
      181 ctggaaatta cgcctttaaa gacgttacct acctggtcga gcatgaagtc acacttcagc
      241 cagtcagcca catcgcgcgt tgcttcgtcg gcacgacgtt ccgccatcga acagtgctga
      301 cccagttgca gcatctcttc catcgaataa tggtagccgc cggtttcagt ggtgttgccc
      361 tgatgcccct gctctttcgc cagcagatat ttaatggcgc ggtgcagcgt caggtctgga
      421 taacgacgaa tcggcgaagt aaagtgcgca taggactgca atgccaggcc aaagtgacca
      481 cggttttctg gatcgtaaat cgcctgtttc atcgagcgca gcagcatggt ttgcagcatt
      541 tctgcatcag gacgatcggc aaccgactcc agcagctccg cgtagtcacg cggttccggc
      601 ttgttaccgc ccggcagttc cagccccagc tccgccagca ctgaacggaa agaggtaatc
      661 gcttcggtgc tcggcttgtc gtgaatacgg aacagtgccg gttctttcgc tttctcaacg
      721 aaacgcgccg ccgagatatt cgccagaatc atgcactctt caattaattt gtgcgcgtcg
      781 ttacgctggg tctgttcgat acgttcaata cggcgttcag cgttgaaaat gaacttcgct
      841 tcttcgctct caaatgagat cccaccgcgt tcttcacggg ctttatccag cactttatag
      901 aggttatgca actcttcgag atgcttaacc agcggggcgt actgctcgcg cagatcctga
      961 tcgccctgca gaatatgcca gactttggtg taggtcagac gcgcgtgaga gctcatcacc
     1021 gcttcgtaga atttgtagcc cgtcaggcgg cctttcgacg aaaccgtcat ctcgcacacc
     1081 atacacaggc ggtctacctg cgggttgagc gaacacaggc cgttagagag cacttccggc
     1141 agcatcggga taacctgcga agggaagtac accgacgtgc cacggttacg cgcttctctg
     1201 tccagcggcg ttgacggacg cacatagtag ctgacgtcgg caatcgcgac ccataaacgc
     1261 cagccgccgc cgcgtttttt ctcgcagtaa actgcatcgt caaagtcacg ggcgtcttcg
     1321 ccatcaatgg tgaccagcgg taaatcgcgc agatcaacac ggcccgcttt tgcttcttcc
     1381 ggcacttctt ctttcagccc ggcaacctgt tgctcaacag cctgcggcca gatgtacgga
     1441 atttcatggg tacgcagagc gatatcaacc gccatgccgg tgcccatatt gtcgcccagc
     1501 acttcgacga ttttacccac cgctttggtg cggcgagtcg gacgctgagt cagttcgact
     1561 acgaccacaa agcccatccg cgcgcccatg atctgatcgg gcgggattaa gatatcgaag
     1621 ctcagacggc tgtcgtcagg aaccacaaag ccgacgcccg cttcggtaaa gtagcgacca
     1681 acaatctggc tggtttttgg caccagtacg cggacaatac gcgcttcacg acgaccttta
     1741 cggtcagcac ccagcggctg agccagcacc tgatcgccat gaatgcaggt tttcatctgc
     1801 tcgctggaga gatacaaatc atctttacgc ccttcaaccc gcagaaagcc gtagccatca
     1861 cggtggccaa taacggtacc tttcaccagg tcgaggcgtt ccggcagcgc atagcactga
     1921 cgacgagtga agaccagttg accatcgcgc tccatcgcgc gcaggcggcg acgcaggcct
     1981 tcaagctgct cttcgccttc aatgtgcagt tctaccgcca gctcatcacg gctggccggt
     2041 ttttcacgtt tggttaaatg ttcgaggata aattcccgac tagggatggg attcgcgtat
     2101 ttttcagctt cgcgttcctg gaaaggatct tgtgacatct cggttcctcc gttgtcatct
     2161 ctgatgaaga ttttcgtcac tccaccagca ataatttata aagcggttga ttctcttcaa
     2221 ccaaatcggc aagcgtgtag ttatccagtt ccgtaagaaa actttgcacg gccttagaaa
     2281 gtgcctgttt caacctacag gcaggtgtaa tgtggcaaaa ctcactgctg caattcacca
     2341 gcgataaggg ctccagctcg cgcaccacat caccaatacg tatcgcactc gccggtttac
     2401 ccaggcgaat gccgccattt tttccacgaa cagcagtcac gtagccggca cgactaagtt
     2461 gattgattat tttgaccata tgattacggg agacgccgta gacgtcagtc acttcagaaa
     2521 tactggtcat ccgcccttct ggcaatgacg ccatgtagat cagcgcacgt aatccgtaat
     2581 cagtgaaact cgttaactgc acatcaacct caaaagggaa atcgggaaaa aatacattta
     2641 tattgatgat aaaccagcca caagctgtgt cgctaattta tttcagttcg gggaaggaaa
     2701 aaagcgagga tttaacactg tgtcggatag cgtaatggct tacccgacac agcaatgtat
     2761 cacactgttc gcccggcagg caaaatatct gccaggcgta ccagaattac gcgtcgaacg
     2821 ggtcgcgcag aatcatggtt tcagtacgat ccggaccggt agagatgata tcgatcggca
     2881 caccagtcag ctcttcaata cgcttgatat agttcagcgc cgcctgcggc aggccgctac
     2941 gatctttcac gccgaaggtg gattcagacc agcccggcat ggtttcgtaa atcggctcta
     3001 cacctttcca gtcgtcagct gccagcggag tggtagtcac ttcgcgacca tccggcatac
     3061 ggtaagccac gcagagttta acctctttca ggccatccag aacgtccagt ttagtcaggc
     3121 agaagccaga cagggagttc agctgtaccg cacgacgaac ggcaacggtg tccagccagc
     3181 cggtacgacg acgacgcccc gtagttgcgc cgaattcgtt accctgcttg cagaggaact
     3241 cgccagtttc atcaaacagt tcggtcggga acggacctgc acctacacga gtggagtaag
     3301 ctttgaggat acccagaacg taatcaacat aacgcgggcc caagccggaa ccggtcgcca
     3361 cgccaccagc agtggtgttg gaagaagtta cgtacggata agtaccgtgg tcgatatcca
     3421 gcagcgtacc ctgcgcacct tcaaacatga cgaaatcgcc acgctgacgc gcctggtcga
     3481 gcaggtcaga aacgtcaacc accatagaag tcaggatgtc ggcaacagcc atcgtatcat
     3541 ccagaacttt ctggtaatca accgcttcag ctttgtagta gttaaccaac tggaagttgt
     3601 gatattccat cacttctttc agtttttcag cgaaggtttc tttgtcgaaa aggtcgccaa
     3661 cacgcagacc gcgacgtgct actttatctt cataagcagg cccgatacca cgaccggtgg
     3721 tgccgatcgc tttcgcgcca cgcgctttct cacgcgcgtt atccagcgca acgtgataat
     3781 caaggatcag cggacatgct tcagacagca gcagacgctc acgaacgggg atgccacggt
     3841 cttccagttc tttcatctct ttcatcagcg cggccggaga cagcacaaca ccgttaccga
     3901 tgatgctggt tacattctcg cggagaatac ctgatggaat aagatggaga acggtttttt
     3961 caccgttgat tacgagagta tggcctgcgt tgtgaccgcc ctggtagcgt acaacatatt
     4021 tagcccgttc agtcagaaga tcgacgatct tacctttacc ttcgtcaccc cattgggtgc
     4081 ccagtacgac gacgttgtta cccatttttc aaaatcaccg tttgcttaaa aatggattct
     4141 accatcgctt tttcagagtt acagcacttt ttgcactcaa aaatgacctc gatcagtctg
     4201 ttttttgttc agccaatcgt tttcctcaac atgtagtaga ccacaacgcc cgcaaccaca
     4261 agtccaccgc caaaacgacg taaaatatta tcgggcaaat tggtcatcgc agagatcatc
     4321 ttcttccatg ccttcgggta aagcatcggc cctaaacctt ccagtaccaa aaccagggca
     4381 agcgccagcc agattgtcga attcattcat tatccttata gaaaaagaaa accaccgaca
     4441 tccctgagga tgcggtggct ttattgacct gtaccgcagt cgttatatta acgcgttgcg
     4501 gaagtcggcg tcttcatgta gcggaagaaa tcgctatccg ggctcatgac catcacgtcc
     4561 tgattgccaa agaagctgtt ctcataagca cgcaggctac ggatgaatgc gtagaagtcc
     4621 ggatctttac tgaatgcatc agcaaacagt ttggctgctt cggcatcgcc ttcaccacgc
     4681 atgatgcggc cctgacgctc agcttctgcc agcgttctgg tcacttcata gtcggcagtc
     4741 gcgcgcagtt tttccgcttc ttcctgacct tgtgaacggt gacgacgcgc taccgcttca
     4801 cgctcggcgc gcatacggtt gtagatcgct tcagacactt cggtcggcag gttgatctgc
     4861 ttgatacgca catcgacaac ttcaataccc agcgccgcca tactgttcgg gttgatgacc
     4921 ggaactttgc ccttcgtctc agccgttacg cgctctgccg cttcggcaat ggcgttatct
     4981 gccgccgggg tagtaacttc atcttctgta cccgcagaac cggagttcag cgcgtcacgt
     5041 acttcgaggg tcagacgacc acgggaatcg gtgacgatat ctttcacgtc caggcgacca
     5101 atttcagaac gcagacggtc agagaactta cgtttcaaca gcacttccgc ttgcgaaatg
     5161 tcgccaccac ccgttgccag gtagtaacgg ctgaaatcgc tgatgcgcca tttgatgtaa
     5221 gagtcgacga tcaggtcttt cttctctttg gtcacaaagc ggtcggcctg gttgtccatg
     5281 gtctgaatac gtgcgtcgag cattttcacc gtttcaatga acggtatctt gaaatgcaga
     5341 cccggctcat aaaccagagg tttgttgtca tcgtcacgca gtaccttacc aaaacgcagc
     5401 gtaataccgc gctcaccttc tttgacgaca aagacagaca tgtaaagcac taccagcacg
     5461 atgatgataa tcgcgataac tgacttacgc atcgttattc cccctgacgc tggtagtcgt
     5521 tacgctgcgc gttggcgcgg cgttggtcca taatatcgcc ctgactggtg gacgacgtgt
     5581 tgcttgctcc actggttgtg gaggaagagg ctggcggcag acgcagcaga ttgctggcac
     5641 cgttatcgct cttcgccgca ggggcgttac cacctttcag catctggtct aacggcagaa
     5701 ccatcaggtt gccaccttta tcgttaacca gcactttgcg ggtgttaccc aacacttttt
     5761 ccatcgtctc gatatacaga cgctcgcgag taatttccgg cgcggcttta tattccggca
     5821 gaagtttagc aaagcgcgcc acttcaccct gagcttccag gatggtctgg gccttgtacg
     5881 cacgcgcctc ttcgaggata cgttgcgcct gaccgttcgc acgcggctga acttcgttgg
     5941 tatacgcttc tgcttcacga atgtattgct gttcgttttc acgcgcggca atcgcatcgt
     6001 caaacgccgc ttttacttct tccggcggac gagcagcctg gaagttgacg tccagcagcg
     6061 tgatacccat gtcatacgga cgaatcgtct cttccagttc gcgctgagta tcgctacgaa
     6121 tcacggtacg accttccgtc agaatgcggt ccatggtgta tttaccgata actccacgca
     6181 gggcgctgtc ggtagcctga cgcaggctgt catccgggct ggtcacgcta tacagatatt
     6241 tttccggatt ggtgacgcgg tactgcacgt tcatctcaac gcgcactacg ttctcgtccg
     6301 acgtcagcat cacaccagaa gcggccagtt cacgcacggc ttccacgttc accggtttga
     6361 cttcgtcgat aaacgtcggt ttccagttca gacccggctc aaccagatgg ctgaatttac
     6421 caaagcgtgt taccacgccg cgttcggctt ctttaatggt atagaaacca ctggccgccc
     6481 agataatgac aatcgctgcc gctgcgatgg taacgacacg accgccaagc tgcgggcgcg
     6541 ggccttgcga tgaactgcca ccgccagatc cggtgccttt accgccgccc agaccaccga
     6601 gctttttgct cagtttgcgg aagatatcat ctaaatcagg tggcccttga tcgcgaccgc
     6661 ctttgtttcc atttccctca gagttgccgc caggtttgct gcttccccac gggtcgcggt
     6721 cttgtccgtt attaccgggc tgattccacg ccatgtttgt gctccatatt tgttatgcgg
     6781 tgatccctgt tgtctttcga cgccagagga tatgactcca cgcttcagac gctacgccgt
     6841 tagatcaggt aatcgatcaa cgccggttct tgtttacaga ggcgacgcca gtcaacgatc
     6901 ggcatacgaa cttgcagact tacgctgccg tcctcctcca tccactcttt ttctattgcc
     6961 tgaagctgat aaaaacgact tctcagacgc ccttcctgcg gtggcagacg caatgtatgc
     7021 tgcgccacct cgccggaaag ccgctccgtc aaagcctgaa aaagctgtgg tatccccgct
     7081 ccggtctgtg cggaaagcca gacacggatc ggtttgttct cttcgtcccg atcaatacgc
     7141 ggttcgaaat cttccagcat atcgatcttg ttcatcacca gcagggttgg gatctcgtga
     7201 gcgtcgatct cttcaagaac cgtattcacc gcttcgatgt tttcttgtac acgcacatcc
     7261 gccgcatcaa tgacgtgcag cagtaatgtg gcttgccgcg tctcttgtaa cgtggcttta
     7321 aatgccgcca ccagatcgtg cggcaggtgg cgaataaacc ctacggtatc tgcaagtacg
     7381 gtttcaccga catctgcaac gtcaatacgc cgcaacgtcg ggtcgagggt ggcaaacaac
     7441 tggtctgccg cgtagacccg cgcttcggtg atgcgattga aaagggtaga tttaccggcg
     7501 ttggtatatc ccaccagcga aacagtagga acgtcggctt tgatacgcga ttgccgcccc
     7561 tgctcacgct gcttttcaac tctttccagg cgcgactgta tctgcacgat gcgattacgc
     7621 aacaaacgac ggtcggtttc gagctgggtt tcacccggac cacgcaaacc tatcccgcct
     7681 ttctgtcttt caaggtgggt ccagccacgc accaggcgcg tagccagatg gcgcagctgc
     7741 gccagctcaa cctgcaactt accctcatgg gtacgcgcac gttgggcgaa aatatctaaa
     7801 ataaggccgg tgcggtcgat aacacgacac tcgcacaaac gctccaggtt acgctcttgc
     7861 gccgggctca gggcatggtc aaaaagaacg accgaagcac ccgtcgcttt gacagcttcc
     7921 gcaatttcaa ctgctttacc ttcacctaca aaatactttg ggtgcggcgc tttacggcta
     7981 ccggtaatca cctgcaatgc ttcgacaccg gcggaagaga ccagagattc aaactcctgg
     8041 aggtcttcca tatctttgtc ttgcgtaaaa tagatgtgta ccagtaccgc ctgctcacca
     8101 gcatcataac ggtcaaacaa gcgtataacc ctctaaatag atcagcgggg aacgcaggat
     8161 cgctggctcc ccgtgtaaaa aaacagcccg aaaccttatt cggtttcttc gctgtcctgt
     8221 tgcgcggaag tattctgcgc gctgctacca tgatggtagt tactgctggt accgccaccg
     8281 gcgttgttac tgtgatgaga aaccgggcga gacgggacaa cagtagaaat cgcgtgcttg
     8341 taaaccatct ggctgaccgt gtttttcaac aggatcacga actgatcaaa agactcgatt
     8401 tgcccttgca gcttaatacc attcaccaaa taaatagaaa ctggaacacg ttcccgacgc
     8461 agtgcgttca ggaacggatc ttgtaaagat tgccccttag ccattctctc ttttccttat
     8521 atgcttattt gtactttgaa cctttcgatt ctgaaaaaat tgcgcacgat acgtctcaat
     8581 tgtacacatt cagcctgcga tagcaccaac aacctgtaat acttcgtcac gcgcctgttc
     8641 tggtttttca ctgtcaagcc agtgaacccc ttcccaacca cgcagccagg ttatctgccg
     8701 cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc atttcatcgt atgagatttc
     8761 gccttcaagg taagaccaca tctggcgata acccacgcaa cgaatggaag gcaagtccgt
     8821 atgcaaatct cctcgggcaa aaagcgcccg gacttctgct tcaaaacctg aagccaacat
     8881 ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca cggctcgccg gggcgatggc
     8941 gaactgatgc acctgatacg gtagagcgtc tcctgacgtt tgcgtcagtt ccgttaaagt
     9001 tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc ctttgtggat catttggatg
     9061 aatccttgct gccgcaaccg gatctacctc ctgaagttga cgatgcaatg actcccaacc
     9121 ttgctctgcc gcctgttgct caattctggc ccgtacttcc gggtctgccg acggtagcgg
     9181 cgacaaccct tccagcaatg ccttgaaata caacatcgta ccgcccacta acagtgggat
     9241 ccgccccgcc gcggtgatat cggccatttc cgccagcgca tcgcggcgaa aatcagcagc
     9301 cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg tgcggcgcgg cgagtaactc
     9361 ttcagcgttc ggcttcgccg tcccgatatc catccctttg taaataaggg cagaatcaac
     9421 gcttatcaac tctactggta aaattttacg cagctcaatg gctaacgccg ttttaccgga
     9481 ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc gccttactga tatcactcat
     9541 ctttcagggc ttttatcgcc ggatgtaaat caacagattg taacagacca cccggcggcg
     9601 ttttcacaag ttgcggacat aaccgttcca cgtccgccag cagggttatg gcctgtgcca
     9661 ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat ccactgcgca atattgccag
     9721 gttcgaatac ggactgcttc gccaggtagc ctatcagttc aggaatcaag atttgtaaat
     9781 tttgttggcg taagggtaaa ggcactgccc tgatggtcac atgctgtgca tctgactgga
     9841 aatcaatacc caattccgcc agggcagact gcgctttttc taatgccgat ttttcttcgg
     9901 cagaaacttt tagccgcaac ggaatcagca gcggctgggc gcaaacgggc gcttcacccg
     9961 gcgtcaattg tgcctgacgc agccaacgtt ctgccactgg caaggataaa agtgaaatgt
    10021 tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat agtcagtacc cgaccaaaac
    10081 tctgactgtt cgccgcaagt gcaggttcct gcggttccgg cgcttttaat ttttgcatcg
    10141 gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg ctgtttctgg tagcctggct
    10201 gcgcattcgg ccagggggca gccggacgac tgcctgatgc tggcgcagga gtgtagcgcg
    10261 gagctaccgg ctcacgagct gccggttctg caaagtgatt gcgccccgcc gccacgcggt
    10321 tttccggaat ggaacgcggt gcaggttggg gttcatcgtc cagcggtagc ggcgtttcca
    10381 gttgctgttg tagcacgctc agcacgccct gatagataaa atcatgcacc agacgcgact
    10441 gatggaaacg cacttcgtgt ttggcggggt gcacgttgac gtccacctga tgtgggtcga
    10501 tctccagata caacacaaat gccggttgct gatcggcccc cagtttgtct tcgcaggcct
    10561 ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg accgttcacg tagcaatact
    10621 gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc cacccagccg cgtagcgtga
    10681 gatcgccgtg ttgccattca atcgccagcg cttgttcaag aaaagcggtg ccgcaaatcg
    10741 cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc gcggtactga cgcacaattt
    10801 taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc cagcgcaatg cggcggatga
    10861 tctcatcaat gtggttaaat tcggttttct cggtgcgcag gaatttgcgc cgcgccgggg
    10921 tgttgtagaa cagatccagc acctccagcg tcgtccccac aggatgcgcc gccggtttta
    10981 ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca ggcttcctgc tgttctgcgg
    11041 tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact cgccagcgcc tcaccgcgaa
    11101 agcccaggct gataatggct tcgagatcgt ccagagaggc gattttactg gtggcatgac
    11161 gagccagcgc cagcgccagc tcatcttttt tgataccgca gccgttatca cgaatgcgga
    11221 taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg cgtcgcacct gcatcgaggc
    11281 tgttttccac tagttctttg actaccgacg caggtcgctc gaccacctca cctgcggcaa
    11341 tctggttcgc cagttgtggc ggtaagacct gaattggcat caatcgtcct tagtttggca
    11401 gcgtgcgatc tggcgtcgtc accgtactgg cagtttgtgc cgttgcaccc tgcggcgcag
    11461 attgcatcgg atgcgcaagg aagtaattgc gcaggccttt gtaaatggct tctgccagct
    11521 gttgttggta atcgtcgctc gccagcaaac gttcttcgct gttgttgctg ataaaaccgg
    11581 tttcgaccag tactgatggg atatccggcg agcgcagaac gccaaggctg gcgtgttctg
    11641 gtcgacgttt atgtatttcg ccaatgcgtt gcaactgact gatcatactg gtcgctacat
    11701 cataccctac ccgctgggaa tgaccgaact gtaaatccag caccgcctgg cttaaatagg
    11761 ggtcagactg actgttcgcc agcacatcac ccgccccacc cagcagctcc gactgtttct
    11821 cgtgctgctc cagccagctg gccatttcac tgttggcgcg acggttagag agcacccata
    11881 cggaagcgcc agtcgcactg cggttcggtg cggcatcagc gtgaatcgac acgaggaaat
    11941 tggcgttttg cttacgtgcc acatcactgc gccccatcac cgagataaag taatccccgt
    12001 cacgggttaa aacgccttta aacatcggat cgtcattgag caaagtacgc aatttacgcg
    12061 cgatggcgat ggtgacattt ttctcccgcg taccaccggg gccgatagcg ccagggtcct
    12121 gaccgccgtg tccggcatca atagcgatga taattttatc gccagtgtta gccgtcgcgc
    12181 gcgctgccgg acgcgttacc gtattactgc tgataacacc cgtagtgcgg ttactttccg
    12241 ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc gacaaccgca ggcgtttcaa
    12301 cgcgtttcgc aaccacgggc ggcggaggag gcggtggcgg cacatcggcg ttaatcgtaa
    12361 agacgacagt gtaattgctg ccattctgcc gcttcaccgc ttcggtttta ccgttttcgg
    12421 taagatcgac caccagccgt agcgtttgtg catctttagg cgttccagag cgaatcgcct
    12481 tcaccagatt attgccgctg aacaacaacg gcagtccctg aatcacgccc gtttgtttga
    12541 tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc ataatcagga tcgccaataa
    12601 aactcaacgt tatccgcgcc tgttgattac cgttagaaac ctgaatatca gagagcgtcg
    12661 cggcacccac cggcgtgcac agcagcagca gcgtcgctac caaccaattt ctgatgcgat
    12721 acatcatccc gccacctttc aaaggttaac cggctaaacg cgccagcaac aattcacccg
    12781 cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc ctgataatca atgtgtattt
    12841 cgacatccgg gtcaggaaga acacctgtac cttgttgtgg ccactccacc aggcagatgg
    12901 catcgttggc aaaataatcg cggatcccca taaactccag ctcctcggga tcggcaaggc
    12961 ggtacaaatc aaagtgatag accattaagt tgtcgagcgt atagggttcg accagcgtat
    13021 aagtggggct tttgacatta ccctgatgac ccagagcctg taaaaagccc cggctaaagg
    13081 tggttttacc tgcgcctaaa tcgccataca gatagattac ggttgcgcca tcgcaggctt
    13141 tcgctacccg ctcgcccagg tctaatgttg cctgctcatc agggagcgga attactcgat
    13201 tcatcatggt ttttatcagt cacttccggg ttaacaatac gctgtagcgt ggaaaagaga
    13261 tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca gtacgtcagc tgccgcaccg
    13321 tgcgcgacac agcctgcaca ggctgcatca tacggcgaca gtttttgccc aagcaatgcg
    13381 ccaataatac cagagagcac atcgcccatg ccgccgctcg ccatgcctgc atttccggca
    13441 tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg ttccggcacc tttcagcacc
    13501 gctacgccgc cataacgttg taccagacgt ttggcgcaat gtaagcggtc actttcaatt
    13561 tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag gatgcggcgt gatcacgcga
    13621 ttgtgacgct tatcgggatt gattgccagc aggttcaatg catcggcatc ccacaacatc
    13681 ggtttgcgaa aattctcaac tttttgcagt gcttttttcc cccactcttg ctggcccaga
    13741 ccgggaccaa tcaccaccac atcggcccat tccaggcttt cggtaagaga gtccatcgtc
    13801 agttcatgca ccatcaattc cggtcgtgca gtcagcagcg gcgcaatgtt ttcactgcgg
    13861 gtcagtactc ggactaaacc agcaccagca cgcagcgccg cttcccccgt catacgaata
    13921 gcccccgccg tgccgtgatc gccaccgata attaccagcc gcccgtgatc gcctttatgc
    13981 gaagtcgggc gacgcggttt tagccagtga gaaagttgtt ctgctgaaaa ccgctgaatt
    14041 ttcgtctcct gacctgccag ccaactatcc agccccagtg agtcaaaatg cagttgtccg
    14101 gtaacatccc gcgcttttcc agtgagcaag cctggtttca gcgcaataaa agtgatggtg
    14161 tgatcggcgt tgatcaccgc gcctggcgta gcgccagttt cagccagcag gccggaaggg
    14221 atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt ggtcgattaa ctggctaatg
    14281 gattcgcggg gcgcttgccg caaaccggta ccgagcagcg catcaacaat cagatctacc
    14341 gattcgggcc agacaatatt cgaagcatgg atctcgccac ccgcgtataa ccatgcttcg
    14401 cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct cctgggccaa caacgtgacc
    14461 tcaatgccga ccgctttggc cagtcgcgcg accacgtagc catcgccgcc gttattacca
    14521 tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg ccgaacgaca cacctggaat
    14581 gcggcctcgc cagcgcgaag catcagctca tagagtgtga gccccagcac atctgccgcc
    14641 tcgcgttctc cgcggcggat atcgtcggcg taccagacgg tgtgtggtat acttacgggg
    14701 tttttcttca ttgtatggtc cgtcatgtca gagcccctcg atctcaatca gttagcgcaa
    14761 aaaattaaac agtgggggct ggaactgggc tttcagcagg taggtattac cgataccgat
    14821 ctcagcgagt ccgagcccaa actgcaagca tggctggaca aacaatacca cggcgaaatg
    14881 gactggatgg cacgtcacgg tatgctgcgc gctcgccctc atgagttatt gcccggtacg
    14941 ctgcgcgtga tcagcgtgcg gatgaattac cttcctgcta acgccgcatt tgccagcacg
    15001 ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc tgggccgtga ctatcacaaa
    15061 cttctgcgca accgactcaa aaagctgggc gagatgattc agcaacattg tgtttcgctg
    15121 aattttagac cgtttgtcga ttctgcgcct attctcgagc gcccgttagc tgaaaaagct
    15181 gggctcggct ggacaggtaa gcactcactt atcctcaatc gcgaggccgg ttcgttcttc
    15241 tttttaggcg aattgctggt cgatattccg ctgcccgtgg atcaaccagt cgaggaagga
    15301 tgcggcaaat gcgtggcctg tatgacgatt tgcccgaccg gtgccatcgt cgagccatat
    15361 accgtcgatg ctcgccgctg tatctcttat ctcaccatcg aacttgaagg ggcgatcccg
    15421 gaagagttgc gaccgttaat gggaaaccgt atttacggtt gcgatgactg ccagcttatc
    15481 tgcccgtgga atcgctattc acaactcacc acagaagagg atttcagccc gcgtaagccg
    15541 ctacacgcac cggaactcat tgagttattc gcctggagcg aagagaagtt tttaaaagtc
    15601 acggaaggat cggcgattcg tcgtattggt cacctgcgtt ggctgcgtaa tatcgccgta
    15661 gcattaggca atgccccttg ggatgaaacg attttgacag cgctggaaag tcgtaaaggt
    15721 gagcacccac ttctcgatga gcacatagcg tgggcgattg cgcagcaaat agagagacga
    15781 aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc gtctggttcg ggtgattgaa
    15841 aaagggttac cgcgtgacgc ctgattcatc cacagcttgt gaataaaaat aaaaacgcat
    15901 tgcaattcaa gacgaataaa atcgtcaagt gatcgactcg acaaaacgga atcttaattt
    15961 ttaacttatc tatcaatagg ttaaaaaaat actattcacc gtgcgaagtt tctttgaaat
    16021 taagattaca gcctgaggct gtggataact ctgtttacaa gagtttgtca gagacaacaa
    16081 aaaccagcaa taacgcatcg cgtcgctgtg gatattttat tgagagaaga atttggagcg
    16141 ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc
    16201 tattcccgct tgggtggtct gtg
//
LOCUS       DBBGLQ010000096        16181 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.096, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000096 DBBGLQ010000000
VERSION     DBBGLQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.096"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(35..411)
                     /gene="rnpB"
                     /locus_tag="ACIYSB_004079"
     ncRNA           complement(35..411)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACIYSB_004079"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(444..1589)
                     /gene="garK"
                     /locus_tag="ACIYSB_004080"
     CDS             complement(444..1589)
                     /gene="garK"
                     /locus_tag="ACIYSB_004080"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGC5431012.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1686..2576)
                     /gene="garR"
                     /locus_tag="ACIYSB_004081"
     CDS             complement(1686..2576)
                     /gene="garR"
                     /locus_tag="ACIYSB_004081"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGC5431013.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2606..3376)
                     /gene="garL"
                     /locus_tag="ACIYSB_004082"
     CDS             complement(2606..3376)
                     /gene="garL"
                     /locus_tag="ACIYSB_004082"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGC5431014.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAELAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3392..4726)
                     /gene="garP"
                     /locus_tag="ACIYSB_004083"
     CDS             complement(3392..4726)
                     /gene="garP"
                     /locus_tag="ACIYSB_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGC5431015.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5101..6672
                     /gene="garD"
                     /locus_tag="ACIYSB_004084"
     CDS             5101..6672
                     /gene="garD"
                     /locus_tag="ACIYSB_004084"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGC5431016.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6821..7156
                     /gene="prlF"
                     /locus_tag="ACIYSB_004085"
     CDS             6821..7156
                     /gene="prlF"
                     /locus_tag="ACIYSB_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGC5431017.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7156..7620
                     /gene="yhaV"
                     /locus_tag="ACIYSB_004086"
     CDS             7156..7620
                     /gene="yhaV"
                     /locus_tag="ACIYSB_004086"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGC5431018.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(7675..8484)
                     /gene="agaR"
                     /locus_tag="ACIYSB_004087"
     CDS             complement(7675..8484)
                     /gene="agaR"
                     /locus_tag="ACIYSB_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGC5431019.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8733..10013
                     /gene="kbaZ"
                     /locus_tag="ACIYSB_004088"
     CDS             8733..10013
                     /gene="kbaZ"
                     /locus_tag="ACIYSB_004088"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGC5431020.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10036..10509
                     /gene="agaV"
                     /locus_tag="ACIYSB_004089"
     CDS             10036..10509
                     /gene="agaV"
                     /locus_tag="ACIYSB_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGC5431021.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKTAGVECFVQGVPTEPAVDLFKLL"
     gene            10520..11299
                     /gene="agaW"
                     /locus_tag="ACIYSB_004090"
     CDS             10520..11299
                     /gene="agaW"
                     /locus_tag="ACIYSB_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGC5431022.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGIFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11289..12167
                     /gene="agaE"
                     /locus_tag="ACIYSB_004091"
     CDS             11289..12167
                     /gene="agaE"
                     /locus_tag="ACIYSB_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGC5431023.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12185..12619
                     /gene="agaF"
                     /locus_tag="ACIYSB_004092"
     CDS             12185..12619
                     /gene="agaF"
                     /locus_tag="ACIYSB_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGC5431024.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12616..13749
                     /gene="nagA"
                     /locus_tag="ACIYSB_004093"
     CDS             12616..13749
                     /gene="nagA"
                     /locus_tag="ACIYSB_004093"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGC5431025.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPAGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTTPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASLVALDSGLHVQQIWIQGQLASF"
     gene            14100..15254
                     /locus_tag="ACIYSB_004094"
     CDS             14100..15254
                     /locus_tag="ACIYSB_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGC5431026.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15267..16127
                     /gene="kbaY"
                     /locus_tag="ACIYSB_004095"
     CDS             15267..16127
                     /gene="kbaY"
                     /locus_tag="ACIYSB_004095"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGC5431027.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         3872 a         4299 c         4171 g         3839 t
ORIGIN      
        1 caaaaacccg ccgaagcggg tttttacgta aatcaggtga aactgaccga taagccgggt
       61 tctgtcgtgg acagtcattc atctaggcca gcaatcgctc actggctcaa gcagcctacc
      121 cgggttcagt acgggccgta ccttatgaac ccctatttgg ccttgctccg ggtggagttt
      181 accgtgccac ggactgttac cagccgcgcg gtgcgctctt accgcaccct ttcaccctta
      241 cctgatcccg cttgcgcggg ccatcggcgg tttgctctct gttgcactgg tcgtgggttt
      301 cccccccagg cgttacctgg caccctgccc tatggagccc ggactttcct cccctccgcc
      361 cgtctccccc gaagaggacg acgacgaagc ggcgactgtc tggtcagctt cggcgcgcag
      421 tatagagggt ttgcgcgccc ttgtcacccc gcgttgcgca ttccaatcgc cagtgtcgcg
      481 gcgatattac gtgaagcacg gcagatattg tcataagccc cgcggaatgc ttcgtccaac
      541 gtacctatgc tggtcaatac gctgaagacc gcatcaatgc catgctgatg tacaacgcca
      601 acatcatcgg tcaggctacc cgcaatgcca atcaccggtt tatggtactt cttcgccacg
      661 tttgcgacac caatcggtac cttcccgtga atactctggc tgtcaatacg cccttcaccg
      721 gtgatcacca gcgtacaatc gtgaatatgt tcctccagat tcagcgccgt agtgacgatt
      781 tcaataccac ttttcagttc cgcaccaaga aacgccatta gcgccgcgcc cataccaccc
      841 gcagctcctg caccggggac atctttcaca tcaacatgca gcgctttttt aatgacctcg
      901 gcatagtgag agaggttatt gtccagctca acaatcatcg cttcactggc tcccttttgt
      961 gggccaaaga tgcgcgatgc gccgttatcg cccaccagcg gattggtgac atcacaagcg
     1021 acgcgaatga cgcaatcttt taagcgcgga tcgaggccgg aaatatcaat atcattcaga
     1081 gtattaagac taccgccgcc aaaaccaatt tcattgccgt tggcgtcgca taatttcgcc
     1141 cccagcgcct gtaccatgcc tgcgccgcca tcatttgtag cgctgccgcc aatgccgata
     1201 ataatgtttg tcgcaccgct ctccagcgcc tgcaggatta actcgcctgt gccgcgtgaa
     1261 gtggtcacga gtggatcgcg tttttccgca ggtaccagct ccagcccact ggccgccgcc
     1321 atttcaataa acgcggtttt gccatcgccg gagatccccc aactggcatt cactttctcg
     1381 cccagcggcc ctgtaaccca ggcgtgacgt tcagccccct gggtggctgc aatcatcgct
     1441 tccaccgttc cttcgccacc gtcggcaacc ggaacagaaa cgtactgtgc atcaggaaaa
     1501 atttcccgaa atcctttttc tatcgcctgc gcaacctcgc tggcagataa actttcttta
     1561 taagagtctg gggcgattac gattttcata cctatgcctg ttaccacatg acgccggagg
     1621 gcgtttctct tattcggcct ggattccagg cccggattgc aatacgccat ccgggcacga
     1681 cgtcattaac gagtaacttc gactttcgcc agtttttcgt agtagcacgc cagggcgcta
     1741 tgatccgccg ttcctaaacc atctgctcgc agtgcctgca tcatctccat aaccgcagct
     1801 gtgagcggca gttgtgcgcc gacgccgtga gaagtatcca gcgcattcgc cagatcctta
     1861 atatgcagat caatacggaa gcccggcttg aagttgcggt ccatcaccat cggcgctttg
     1921 gcatccagca cggtactgcc cgccagtcca ccgcgaattg cctgataaac caggtccggg
     1981 ttaacgcccg ctttagttgc cagcgttaac gcttctgaca tcgcggcaat attcagcgcc
     2041 acaatgacct gatttgccag tttggtgacg ttacctgcac cgatttcccc ggtatgcacc
     2101 acggaacccg ccatcgcttt catcaaatca tagtatttgt cgaaaatagc cttgtcgccg
     2161 cccaccatca ctgacagcgt accgtcgatg gctttcggtt caccgccgct caccggagca
     2221 tccagcatat caatgccttt cgctttcagc gcttcgctga tttcacggct tgccagcggt
     2281 gcgatagaac tcatatcgat caataccgta cctggcttcg cgccttcaat aatgccattc
     2341 tcacccagcg ccacctcttt cacatgaggg gagtttggca gcatggttat gatgacgtcg
     2401 cactgttcag cgatcgcttt agccgtagac gctgtttctg cacctgcagc aatcacgtca
     2461 gcaatagctt ctgggttacg gtcagcaacc accagcgagt aacctgcttt cagaaggttt
     2521 ttactcattg gtttacccat aatccccagg ccaataaaac caactttcat agtcatatca
     2581 atcatctctc ttgttgcggt ggtggttatt ttttaaaggt atcagccagt ttctgagtgg
     2641 cagagcggaa gacgccgaga tcgctgccga cagccacaaa cgtcgcgccc cattccagat
     2701 aacgacgcgc atcggcttcg accggcgcga ggataccgct gggtttgccg tgcgcgctgg
     2761 cacggttaaa aatgtgctga attgcttttt gtacatccgg gtgtgatgca ttgccgagat
     2821 ggcctaatgc cgcggccaga tcgctggggc cgacgaagat gccgtctacg ccttcggtag
     2881 cggcaatggc atcgacgtta tctacgccct gctgactttc tatctggacc agaatagtga
     2941 tgttcttgtt cgactgagcg aaataatccg ccacggtgcc aaacatattg gcgcggtgag
     3001 aaacggagac gccgcgaatg ccttccggtg ggtaacgggt tgatgccacc gctagctctg
     3061 cttcctcttt tgtttctaca aaaggaatca ggaagttata gaaaccgata tccagaagac
     3121 gcttaataat taccggctcg ttggtcggca ctcgcactac tggcgcgctg gcgctgcctt
     3181 tcaaggccat taactgcgga ataaacgtgg agatatcgtt tggcgcatgt tcgccatcca
     3241 gcaccagcca gtcaaaccca gccaaaccaa gaacttcagt gctaatcggg ttagagagtg
     3301 ctgaccagca accaatttgt acctgtttcg cagccagtgc ggctttgaat ttattcggga
     3361 aaacatcgtt attcatcgct tatacctttg cttatttctg caattccata cgtttaatgt
     3421 cgccaactac gaagaggtag cagaccatcg ccatcagcgc tgaacatccc acgaaaacca
     3481 gtgctgcatt gaaggagtgc agttcactta ccaggtagcc aatcaccagt ggagtgacaa
     3541 tggaggcaac attgccaaag acgttaaaga cgccgccgca gaggccaaca atctctttcg
     3601 gcgcggtgtc agaaatcacc ggccagccca gcgcaccaaa tcctttgcca aagaaagcca
     3661 gcgccatcag catgaccacc agcgtggtgt tgttggtgta gttacataag atgatggtgg
     3721 aagccagcaa cattcccagc acaatcggta gcttacgtgc cagggtcagg gataaaccgc
     3781 gtttgatcag ataatccgag aagacacctc ccagcacgcc gcccgcaaaa ccacacagtg
     3841 ctggaatcga ggcgaccaga cccactttca gaatcgacat gcctttttcc tgcaccagat
     3901 aaatcgggaa ccaggtgagg aagaaccagg tgatggtgtt gataaaatat tgtccgaaaa
     3961 atacgcccag catcatacgg ttagagagca attgcttgat gtaatgcagt ttgggtccgc
     4021 ttgctgccgc actgcccggc tttttgtggt ccatatcgac caccgcgcca ttttcagaga
     4081 taaacttcag ctcttccgca gacatacgtg ggtgatctgt cgggttatga atcaacttga
     4141 tccacagcgc cgtcagcaca aaaccaatca cccccataac ggtaaagacg tgctcccagc
     4201 cccaggcgaa agtcagccag ccaagcagcg gcgaaaagag cgccagcgag aaatattgcg
     4261 ccgagttaaa gatggcggag gcagtaccac gttctttcgt cgggaaccag gcggcgacaa
     4321 ttcgggcgtt cgccgggaat gatggcgctt ccgagaagcc gagcataaag cgcataaaga
     4381 acatggagat ccctgcccag gccagcggga acatatcaac aaagccttgc aggaaggtga
     4441 atagcgacca gaaaaagagg ctgtaggtgt aaactttttt cgagccaaac ttatcaagca
     4501 gccagccgcc ggggatttgc atcagcaagt aggcccagcc aaaagcggag aagatgtaac
     4561 ccatcgaaac cgcacttaac tgcaactctt ttgccacttc ggtaccagca atagacagcg
     4621 ttgcacgatc ggcgtagtta acggcggtaa caataaaaat aatcagtaat attaaatagc
     4681 gggtatgcac gcctttcttt ttttcgtcaa cggtgtccag aatcatttta tttacctcgg
     4741 gtacttatgc tgatttttat tattatgggg aaggtgttat ttatgagttt catttatgcc
     4801 gtaacgacaa tgaactcggg aattagtata agcagcgcga gaataataat cattgtgcaa
     4861 atgctaattt aattaatact atttaaatat tattttgagc atatgcacat aaggttgcgc
     4921 gctaaagcac agatttgcgc tttaccttac cgggcggcac tgcaatccct gaaatgattg
     4981 acattgatca catttctgcg tttaaactcc tgacattctt atttcaccca atgaagtcat
     5041 ttatttttaa atgagaccag gtcctcattt taataacccc tggctggaga atattgcaca
     5101 atggccaaca tcgaaatcag acaagaaacg ccaactgcgt tttatataaa agttcacgac
     5161 acagataatg tggcaattat tgttaatgat aatggcctga aagcaggaac gcgttttccg
     5221 gatgggctgg aattaattga acatattccc caggggcata aagtcgcatt gctggacatt
     5281 ccggctaatg gtgaaattat tcgttatggc gaagtgattg gttacgccgt gcgtgcaatc
     5341 ccacgcggaa gctggatcga cgaatcaatg gttgtactac cggaagcgcc gccgttacac
     5401 acgctgccac tggcaaccaa agtcccggaa cccttaccgc cgctggaagg atacaccttt
     5461 gagggctatc gcaatgccga tggcagcgtg ggcaccaaaa acctgctcgg tatcaccacc
     5521 agcgtccact gtgtggcagg cgtggtggac tatgtagtaa aaatcattga acgcgatctg
     5581 ctaccgaaat acccgaacgt cgatggcgtg gtggggctga atcatttgta cggttgtggc
     5641 gtggcgatta acgcaccggc ggcagttgta cctatccgta ccattcacaa tatttcgctg
     5701 aatcctaact ttggcggcga agtaatggtg attggcctgg gttgtgaaaa gttgcagcct
     5761 gagcgcctgc tgactggaac ggatgatgtg caagctattc cagtagaaag cgccagcatt
     5821 gtcagtttgc aggatgaaaa gcatgtcggt tttcagtcca tggtcgagga tattttgcag
     5881 atcgccgaac gccatctaca aaaactgaat caacggcagc gagaaacctg cccggcttca
     5941 gaactggtcg ttggtatgca gtgcggtggc agcgatgcgt tttctggtgt aacggcaaac
     6001 ccggcggttg gctatgcgtc tgatctactg gtgcgctgcg gcgcaacggt gatgttttca
     6061 gaagtaacgg aagtgcgtga cgcgatccat ctgctgacac cacgcgcagt gaacgaagag
     6121 gtcggcaaac ggctgctgga ggagatggag tggtacgata actatctcaa tatgggaaaa
     6181 accgaccgca gcgccaaccc ttcgccgggc aacaagaaag gcggtctggc aaacgtggta
     6241 gagaaggcac tcggctccat tgctaaatcg ggtaaaagcg caattgttga agtgctgtcg
     6301 cccggtcaac gcccgactaa acgcggatta atttacgccg cgacgccagc cagcgatttt
     6361 gtctgtggca cgcaacaggt ggcttcgggt atcacagtgc aagtgtttac gaccggtcgt
     6421 ggtacgccgt acggcctgat ggcggtaccc gtcattaaaa tggcaacccg caccgagctg
     6481 gcgaaccgct ggtttgattt aatggatatt aatgcgggca ccatcgctac cggcgaagaa
     6541 actattgaag aggtgggctg gaagttgttc cactttattc tcgacgtcgc cagcgggaag
     6601 aagaaaacct tctcggatca atgggggctg cataaccagc tggcggtgtt taacccggca
     6661 ccggtgacct gatttctttt ggaacgtccc tcgcaaaaca tggccttagt gccatgtttt
     6721 tattgtttaa agcccccacg tccattaata atgcatttgc attaccttag ttcaagctta
     6781 taatttgagc agaaaacagg ctgtaaaagg acagtgaatc atgcccgcta atgctcgctc
     6841 tcacgctgta ctgaccactg aatcaaaggt cacgatacgc ggacaaacaa ctatccccgc
     6901 gccagtgcgt gaggccttaa aactgaagcc aggccaggac agcattcatt acgaaattct
     6961 gcctggtggg caagtattta tgtgccgact gggagatgaa caggaggatc atactatgaa
     7021 tgcatttttg cgttttctgg atgcagatat ccagaacaac ccgcaaaaaa ctcgtccatt
     7081 caacattcaa caaggaaaga aacttgtcgc tggcatggac gtcaacattg atgatgagat
     7141 tggcgacgac gaataatgga ttttccacaa agggttaatg gttgggcgct atatgctcat
     7201 ccctgttttc aggaaaccta cgacgcttta gttgccgaag tcgagacatt aaagggaaaa
     7261 gatcctgaaa attatcagag aaaagccgcc acaaagttat tggcggtagt ccataaagtg
     7321 attgaggagc atatcacggt caatccatca tcaccggcat tccgtcatgg caagtcgtta
     7381 ggctctggga aaaataaaga ctggtcacgg gtaaaatttg gtgctggtcg ttatcgtctc
     7441 ttctttcgtt atagtgaaaa agagaaagtc atcattctgg gatggatgaa cgatgaaaac
     7501 actctgcgca cctacggtaa aaaaacagat gcctataccg tattcagcaa aatgttaaaa
     7561 agaggacatc ctcctgccga ctgggaaacc ctcacccgag aaacagaaga aacccattga
     7621 tggtgttcac aatgcccgcc gaacgcattc gtgttgacca gcgggcatcc tttctcactc
     7681 cccgaccaga atcacttcaa ccccagcctt tcgcagtcct tccaggctat ccgcaggaat
     7741 gccttcatca acaatgatca tgtcgatacg ttgagtatca atgatcttat gtaaactgga
     7801 acgattgaac ttactggaat cggtgaccac gatgatccgt tccgcaactt cgcacatccg
     7861 acggtttaaa cgagcttcat cttcattatg tgtgctgacg ccgcgctcca gatcgatcgc
     7921 atctacacca agaaacagca tatcgaagtg gtaattttgc agcgattgct cagcctgatc
     7981 gccgtaaaaa gattgcgact gacggcgcaa atgcccgccg gtcatcagca gctcaacgcc
     8041 ttccgcttcc agcaacgcat tagccacgtt cataccgttg gtcatcgcaa ttacgtcagt
     8101 gtgcttgcgc atcagacgag caatctcaaa agtggtggtc ccggaatcga ggataacccg
     8161 atgacctggc tgaatcaact caacggcagc tttcgcaacg ctgcgtttca tcgcggtgtt
     8221 cagtgcgctt ttatcttcca ctgatggctc gactgacggc gtcgtgctat cgcagatcaa
     8281 cgcgccacca taggcacgca cagcgatccc ctgcttttcc agaaacgcca gatcgttgcg
     8341 gatcgtcaca gtagatacgc catacaatgc cgacagatcg ttaacctgca cactcccttg
     8401 ctgtcgcaga cgctgaatga tctgttctcg tcgctcgctg gtgccagtca ctcgcttctc
     8461 acctgaagcg tcggtattac tcatagtaag tcctttcgta aaactttcgt ttcatttcgt
     8521 tttgcctatt aacgcctttc tattaagcaa atgcaagccc accttgccca ttgacgcaag
     8581 ctactctcgt ttcagtgact ttcattatgt ttcttttgtg aatcagatca gaaaactatt
     8641 atctttcgtt ttatttttat ctcaccatga cgcagtatca actgaaacaa aacgaaagat
     8701 taatatcgca gtaatctgaa ctggagagga aagtgaaaca tctgacagaa atggtgagac
     8761 agcacaaagc gggcaaaaca aatggaattt atgccgtttg ttccgcacat ccgctggtgc
     8821 tggaggctgc aatccgctac gccagtgcaa accaaacgcc gttactgatt gaagcaacct
     8881 ccaatcaggt agaccagttc ggcggttata ccggaatgac gcccgccgat tttcgcggct
     8941 ttgtttgtca gctcgccgac tcgttgaatt tcccgcagga tgcgttgatt ctgggtggtg
     9001 accatctggg gccaaaccgc tggcaaaacc tgccggccgc tcaggcaatg gccaatgccg
     9061 atgatttgat taaaagctac gttgcggcag gattcaaaaa aatccacctt gattgcagca
     9121 tgtcctgtca ggacgatccg attcccttaa ctgatgacat cgtggctgaa cgcgccgccc
     9181 gtctggcgaa agtggcggaa gaaacctgtc ttgaacactt tggcgaagcc gatctggagt
     9241 atgtcattgg taccgaagtg ccggtacctg gcggcgcgca tgaaacctta agcgagctgg
     9301 cggtcaccac gccggatgcc gcccgcgcca cgctggaagc ccatcgtcac gcctttgaaa
     9361 agcaaggttt gaatgccatc tggccacgca tcattgccct ggtggttcaa cccggcgtcg
     9421 aattcgatca caccaacgtt attgattatc agcccgccaa agcgagcgcc ttaagccaga
     9481 tggtcgaaaa ctacgaaacg ctgattttcg aagcgcactc taccgattat caaacgccgc
     9541 aatcgctgcg ccagctggtg attgaccact ttgccattct gaaagttggc ccagcgctga
     9601 ccttcgccct gcgtgaagct ctgttctctc tggcggcgat tgaagaagaa ctggtgccag
     9661 cgaaagcctg ttctggtctg cgtcaggtgc tggaagacgt gatgctcgac cgcccggaat
     9721 actggcaaag tcactaccac ggtgacggca acgcgcgtcg tctggcgcgt ggttatagct
     9781 actcggatcg cgtgcgctat tactggccgg acagccagat tgatgacgct ttcgctcatc
     9841 tggtacgtaa tctggcggat tcaccaattc cgctgccgct gatcagccag tatctgccgc
     9901 tgcagtacgt gaaagttcgc tccggcgagc tgcagccaac gccacgggaa ctcattatca
     9961 accatattca ggacatcctg gcgcagtacc acacagcctg tgaaggccaa taagcaaaac
    10021 aaagaggaac acgctatgcc aaatattgtt ttaagccgga ttgatgaacg cttgattcac
    10081 ggtcaggtcg gcgttcaatg ggtcggattt gcgggggcaa atctggtgct ggtagccaac
    10141 gatgaggttg ccgaagatcc ggtacaacaa aacctgatgg aaatggtact ggcagaaggg
    10201 atcgccgtac gtttctggac gctgcaaaaa gttatcgaca acattcatcg cgccgccgat
    10261 cgacagaaaa tcctgctggt ttgtaaaaca cccgccgatt tcctgacgct ggtgaaaggt
    10321 ggcgttccgg tgaatcgcat taacgttggc aatatgcact acgccaatgg caaacaacaa
    10381 atcgccaaaa cggtttctgt ggatgcgggc gatatcgcag catttaacga cctgaaaacc
    10441 gctggggtgg aatgcttcgt tcagggcgtc ccgacagagc ctgctgtgga cctctttaaa
    10501 ttactttgag ggattcatca tggaaatcag cctgttgcag gcatttgcgt tgggcattat
    10561 cgcctttatc gctggcctgg atatgtttaa cggcctaacc catatgcacc gcccggtggt
    10621 cctcggcccg ttggtcgggc tggtacttgg cgatctgcat accggaattt taaccggcgg
    10681 tacgctggaa ctggtgtgga tggggctggc cccgctggcg ggcgcacagc cgcctaacgt
    10741 gattatcggt actatcgtcg gcacggcgtt tgccattact actggcgtga aacccgatgt
    10801 cgcagtaggt gtcgccgtac ctttcgctgt cgcagtacag atggggatta ccttcctgtt
    10861 ctcggtgatg tccggcgtga tgtctcgctg cgaccggatg gcggagaacg ccgatactcg
    10921 cggcattgaa cgcgtgaact atctggcact gctggcactc ggcattttct attttctctg
    10981 cgctttcctg cctatctact tcggcgcgga acatgcaaaa accatcattg atgtcctgcc
    11041 gcaacgatta atcgacggcc tcggcgtcgc gggcggcatc atgccagcaa tcggttttgc
    11101 cgtgctgctg aaaatcatga tgaaaaacgt ctacatcccc tacttcatcc tgggctttgt
    11161 tgccgccgcc tggctcaagt taccagtgct ggctattgct gccgctgcgc tggcgatggc
    11221 gctgatcgac ttgctgcgta aatctcctga accgactcaa cctgcggcac agaaagagga
    11281 atttgaagat ggcatctaat caaaccaccc tgccgaacgt cagtgaaaac gaagaaacat
    11341 tgctgactgg cgtcaatgaa aacgtgtatg aagatcagag cattggcgcg gagctgacga
    11401 aaaaagatat caaccgcgtc gcctggcgtt ccatgctgtt acaagcgtct tttaactacg
    11461 aacgtatgca ggcttctggc tggttgtacg gtctgctgcc tgctctgaaa aagatccaca
    11521 ctaataaacg cgacctggcg cgcgccatga aggggcatat gggtttcttc aatacccatc
    11581 cgtttctggt gacgtttgtt atcggcatta tccttgcgat ggagcgttct aagcaggacg
    11641 ttaacagtat tcagagcacc aaaattgccg tcggtgcgcc gcttggcggg attggcgatg
    11701 caatgttctg gctaacgcta ctgccgattt gtggcgggat tggagccagt ctggcattgc
    11761 aaggctccat tcttggcgca gtcgtcttta ttgtgctgtt caacgtggta cacctggggc
    11821 tgcgttttgg tctggcgcat tatgcttacc gcatgggcgt ggcggcgatt ccactgatta
    11881 aagcgaatac caaaaaagtc ggccatgctg cgtctatcgt tgggatgacg gtaatcggtg
    11941 cgctggtggc aacctatgtt cgtttaagca ccacgctgga aatcacggcg ggcgacgcag
    12001 tggttaagtt acaggctgat gttatcgaca aactgatgcc ggccttctta ccgttggtct
    12061 acaccctgac catgttttgg ttggtacgcc gcggctggag tccgctgcgt ctgattgcag
    12121 ttaccgtggt tctcggcatc gtcggtaaat tctgccattt cctttaaata caatgaggta
    12181 ttagatgtta agtattattt tgacagggca tggcgggttt gccagcggca tggaaaaagc
    12241 aatgaagcaa attctcggtg agcaatcgca gtttatcgcc atcgattttc cggaaacctc
    12301 aagcaccgcg ctgcttacat cgcagcttga agaggccatc gcccaactgg attgtgaaga
    12361 cggcattgtt tttctgacgg atttgctggg cggcacgccg tttcgcgtag cttcaacgct
    12421 ggcgatgcaa aaaccgggct gtgaagtgat caccggcacc aatttgcaac tgctactgga
    12481 gatggtgctg gagcgcgaag ggttaagcgg tgaagaattc cgtgtacagg cgctggagtg
    12541 tggacaccga gggctgacca gtctggtgga cgagttaggc cgctgccatg aagaatgtcc
    12601 ggtcgaggaa ggaatatgac acacgttctg cgcgccagaa ggctgctgac tgaagaggga
    12661 tggctcgatg accatcagtt gcgtattgct gacggtgtca tcgcagcaat cgaaccgatt
    12721 ccagcgggcg tgactgaacg cgatgcggaa ctgctctgcc ctgcttacat cgacacccat
    12781 gtacacggtg gtgcgggcgt tgatgtaatg gatgacgcgc cggatgtact cgacaagctg
    12841 gcaatgcaca aggcacgcga aggtgtcggc agttggttac cgaccaccgt aaccacgccg
    12901 cttaatacca ttcatgcggc gctgaaacgt attgctcaac gttgccaacg cggcggacct
    12961 ggtgcgcaag tgctggggag ttatctcgaa ggaccgtact tcacgccgca gaataaaggc
    13021 gcgcatccgc cggagttgtt tcgcgagctt gaaattgccg agctggatca gttgattgcc
    13081 gtttctcagc acaccttacg tgtggtagcg ctggcaccgg aaaaagaagg ggcattgcag
    13141 gccatccgcc atcttaaaca gcaaaacgta agggtgatgc tggggcatag cgcggcgacc
    13201 tggcaacaaa cccgcgccgc atttgatgct ggtgccgacg gcctggtgca ttgctataac
    13261 gggatgacag gtttacatca ccgcgaaccg ggaatggttg gcgcgggatt aacggacaag
    13321 cgcgcctggc tggaactgat agccgatggt catcatgtgc atccggcggc aatgtcgctg
    13381 tgttgttgct gtgcgaaaga gagaatcgta ctgatcaccg acgcgatgca ggcagctggg
    13441 atgccggatg gtcgctatac gttatgtggt gaagaagtgc agatgcacgg tggcgttgtc
    13501 cgtaccgcgt ctggtgggct ggcgggcagt acgctgtctg ttgatgcggc agtgcgcaat
    13561 atggtcgagt tgacgggcgt aacgcctgcg gaagccatcc atatggcgtc actgcatccg
    13621 gcgcgaatgc tgggtgttga tggtgttctg ggatcgctta aaccgggcaa acgcgccagc
    13681 ctcgttgcgc tggatagcgg gctacatgtg caacaaatct ggattcaggg tcaattagct
    13741 tcgttttgat agtttgctcc tttattgggc cttcacttcc cccgtaaggc ctttcttttt
    13801 ctttcgtttt gatctgtgca gcggtgtcgg atgcgacgct aacgcgtctt atccgaccta
    13861 cagttggtga ccgcaaggcc ggataaagcg tttgcgccgc atccagcaat cccttttgct
    13921 tcctttatct tttctttcaa cgatcacaaa tttcgtttta tttctttttt ctccattgaa
    13981 ctttcagttt cttttctata gattttaatc aacgaaagac atcaccaagt gaaatgaaac
    14041 gaaaggcaag tgaaagcgac aacgcccgac gtcaagttca tcagactaag gattgagtta
    14101 tgccagaaaa ttacacccct gctgccgccg caaccggtac atggactgaa gaagagatcc
    14161 gccatcagcc tcgcgcatgg atccgttcac tcaccaacat cgacgcgcta cgttccgcgc
    14221 tcaataactt ccttgaaccg ttactgcgca aagagaatct gcggatcatc ctgaccggag
    14281 ccggaacgtc ggcatttatc ggtgacatca tcgcgccgtg gctcgccagc cataccggta
    14341 aaaacttcag cgccgtaccg accaccgatc tggtcaccaa tccgatggac tacctgaacc
    14401 cagctcatcc gctgctgttg atctccttcg gtcgatccgg caacagcccg gaaagcgtcg
    14461 cagccgtgga actggcaaat caatttgtac cggaatgcta tcacctgccg atcacctgca
    14521 acgaagcggg cgctctttac caaaacgcga tcaacagcga taacgcgttt gccctgctga
    14581 tgcccgcaga aacgcacgat cgcggctttg cgatgaccag cagcattacc accatgatgg
    14641 ccagctgcct cgcggttttc gcacctgaga cgatcaacag ccaaaccttc cgcgacgtgg
    14701 cggatcgttg ccaggcgatc ctgacctcac tgggcgattt cagcgaaggt gtgtttggtt
    14761 acgcaccgtg gaaacggatc gtttatctcg gtagcggtgg cttacagggc gcagcacgcg
    14821 agtcggcgct gaaagtgctg gaactgacgg cgggtaaact ggcggccttt tatgattctc
    14881 caaccggatt ccgtcatgga ccaaaatcgc tggtcgatga cgaaacgctg gtggtggtat
    14941 ttgtctccag ccacccttac acccgtcagt atgatcttga tctgctggct gaacttcgcc
    15001 gtgacaacca ggcaatgcgt gtaatcgcca tcgccgcgga aagcagcgac atcgtcgctg
    15061 ccggtccaca tatcatcctg ccaccgtcac gtcactttat cgacgttgag caggcatttt
    15121 gcttcctgat gtacgcccag acgtttgcac tgatgcagtc gctgcacatg ggcaatacgc
    15181 cggatacccc atcagccagt ggcaccgtta accgcgtggt gcaaggcgta atcattcatc
    15241 cgtggcaggc ataagaggat cgcattatga gcattatctc cactaaatat ctgttacagg
    15301 acgcccaggc caatggctac gcggtgcctg cttttaacat tcataacgcc gagacgatcc
    15361 aagcgatcct cgaagtgtgc agtgaaatgc gatcgccggt gatcctcgcc ggaacgccgg
    15421 ggacctttaa acacatcgcg ctggaagaga tctacgccct gtgtagcgcc tattccacaa
    15481 cctacaacat gccactggcg ctgcatctcg accaccacga atcgctggat gatattcgcc
    15541 gtaaagtcca cgcaggtgtg cgcagtgcga tgatcgacgg cagccacttc ccgtttgccg
    15601 agaacgtgaa gctggtgaaa tcggttgttg acttctgcca ctcacaagat tgcagcgtgg
    15661 aagcagaact gggccgcctg ggcggtgttg aagatgacat gagcgttgac gccgaaagtg
    15721 cattcctgac cgatccacaa gaagctaaac gctttgtcga actgactggc gtcgacagcc
    15781 tggcggtagc gattggtacg gcgcacggct tatacagcaa aacgccgaag attgatttcc
    15841 agcggctggc ggaaattcgt gaagtggtgg atgttcctct ggtgctgcat ggtgccagcg
    15901 atgttccgga tgaatttgtc cgtcgcacta ttgaacttgg cgtcacaaaa gtgaacgttg
    15961 ccacagaatt aaaaatagcc ttcgctggcg cggttaaagc ctggtttgcg gaaaatccgc
    16021 agggtaatga tcctcgttat tatatgcgcg tcggaatgga tgcgatgaaa gaagttgtca
    16081 gaaataaaat taatgtctgt ggttcagcga atcgaatttc agcataatca ttagtttttt
    16141 atgatttatc ccaatgtact tcccgattaa tatcggggag t
//
LOCUS       DBBGLQ010000097        16097 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.097, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000097 DBBGLQ010000000
VERSION     DBBGLQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.097"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            131..1453
                     /locus_tag="ACIYSB_004096"
     CDS             131..1453
                     /locus_tag="ACIYSB_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5431028.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(1635..3695)
                     /locus_tag="ACIYSB_004097"
     CDS             complement(1635..3695)
                     /locus_tag="ACIYSB_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGC5431029.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(3765..4532)
                     /gene="pdeH"
                     /locus_tag="ACIYSB_004098"
     CDS             complement(3765..4532)
                     /gene="pdeH"
                     /locus_tag="ACIYSB_004098"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGC5431030.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            4764..5693
                     /gene="kdgK"
                     /locus_tag="ACIYSB_004099"
     CDS             4764..5693
                     /gene="kdgK"
                     /locus_tag="ACIYSB_004099"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGC5431031.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIVGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(5789..7285)
                     /locus_tag="ACIYSB_004100"
     CDS             complement(5789..7285)
                     /locus_tag="ACIYSB_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGC5431032.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAVWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(7506..8792)
                     /gene="dctA"
                     /locus_tag="ACIYSB_004101"
     CDS             complement(7506..8792)
                     /gene="dctA"
                     /locus_tag="ACIYSB_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGC5431033.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(8975..10963)
                     /gene="hmsP"
                     /locus_tag="ACIYSB_004102"
     CDS             complement(8975..10963)
                     /gene="hmsP"
                     /locus_tag="ACIYSB_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGC5431034.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAKELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPGDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(11045..14518)
                     /gene="bcsC"
                     /locus_tag="ACIYSB_004103"
     CDS             complement(11045..14518)
                     /gene="bcsC"
                     /locus_tag="ACIYSB_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGC5431035.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKSNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(14500..15606)
                     /gene="bcsZ"
                     /locus_tag="ACIYSB_004104"
     CDS             complement(14500..15606)
                     /gene="bcsZ"
                     /locus_tag="ACIYSB_004104"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGC5431036.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFSALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(15613..>16097)
                     /locus_tag="ACIYSB_004105"
     CDS             complement(15613..>16097)
                     /locus_tag="ACIYSB_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGC5431037.1"
                     /translation="LVQATESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAA
                     VIGFQSPYNDQRSVIALLADSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRV
                     GDVYYVGHLPWFERVWYALANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE
                     "
BASE COUNT         3644 a         4556 c         4117 g         3780 t
ORIGIN      
        1 aaatcactgg cctaaacatt atcccctttt tgcctgattt ttgaccattt ccgcgatttg
       61 ttacacattg aaatatcact tttgctgtgc gtaatatggc tattcgttag ccaaaaaata
      121 agaaaagatt atgcaagcaa cagccacaac actcgaccac gagcaagaat acacgccgat
      181 caactcgcgt aataaagtcc ttgtcgcctc tctcattggc acagccattg agttcttcga
      241 cttttacatt tacgccactg cggccgttat tgtgtttccg catatcttct tcccgcaggg
      301 cgatcctacg gcagcaacgc tacagtcgct cgccaccttc gccatcgcct tcgtcgcgcg
      361 ccccattggc tctgccgttt ttggtcattt tggcgatcgc gttgggcgta aagcgacgct
      421 ggtcgcctcg ttgctaacga tggggatttc gaccgtggtg attggtctgc tgccgggcta
      481 tgccacgatt ggtattttcg ccccgctgct gctggcgctg gctcgatttg gtcagggtct
      541 gggcttaggc ggtgaatggg gcggcgcggc gctgctggcg actgaaaacg ccccaccgcg
      601 caaacgtgca ctgtatggct cctttccgca gctgggcgca ccgattggct tcttctttgc
      661 caatggcact ttcttgctgc tttcctggct actgaccgac gagcagttta tgagctgggg
      721 ctggcgtgtg ccatttatct tctcggcggt gctggtcatt atcggcctgt atgttcgcgt
      781 gtcgctgcat gagtcgccgg tgtttgagaa agtcgctaaa gcgaaaaaac aggtgaagat
      841 cccgctgggt acgctgctga ccaaacatgt tcgcgtaacc gtactgggta cgttcattat
      901 gctggcaacc tatacgctgt tttacatcat gacggtctac tctatgacct ttagtaccgc
      961 cgccgcgcca gttgggcttg gcctgccgcg taacgaagtg ttgtggatgt tgatgatggc
     1021 agttattggt tttggcgtga tggtgccagt cgctggatta ctggctgatg cctttggtcg
     1081 ccgtaaaagc atggtaatca tcaccacgct gatcatcctg ttcgcgctgt tcgcctttaa
     1141 cccactgctc ggttctggca acccgattct ggtttttgcc ttcctgctgc tggggttaag
     1201 tctgatgggt ctgaccttcg ggccaatggg tgcgctgtta ccagagctgt ttccgacaga
     1261 agtgcgttac accggagcat cgttctctta caacgtagcg tcgattctcg gggcttccgt
     1321 tgcgccatat atcgcagcct ggttgcagac taactacggg ctaggtgcgg tggggttata
     1381 tctggcggcg atggctggct tgacgttaat cgccctgctg ctgacccatg agacgcgaca
     1441 tcagtcgttg taataagagt gaaaagcctg atgcgctacg cttatcaggc ctgaatttcc
     1501 atacaatata ttgaattcgc aaggatttgt aggccggata aggcgttcac gccgcatccg
     1561 gcatgaacaa agcacacttt gccaacaatc taaatctgtt taattgccgg atgtcagaca
     1621 tccggcaatt atcatcactt cttcatctgc gacaaaatcg tccgacactg attagcctcc
     1681 ccttcggaag gtgagatcag tgccagtaac gccgccgccg gtgttaccag cgttgccagt
     1741 gccgcagcaa cagcaccacg ggcaatcagc ggtccggctt tcaccccagc ctgcggattt
     1801 ttaaacgtcc cccgcacata cagcggcgaa cgcagtgtga taatccgaat tcctttactc
     1861 tccggatcaa tagtcaaatc cagctgttcc gaagcaaaac ttgccgtgcc ggtaacatta
     1921 atcaacgcgt tctcagtatc gaaagcaaaa atctgcgggc gcgccacgcc gttggcaata
     1981 ttcagattcg ccgccgcgca gttcacccgc acctcatcat caccaaatat cgcaccgaca
     2041 atgtagttgc cgacattcag cccaacaatc tccatcaggt tgcggctcac cagcccgtca
     2101 ttcatcaaca gtttcaggtt gccgttactg ttgcctaaaa gtgccgccac cgagttaccg
     2161 ctaccgcgta gttccgcgtc accgttcatt tcccccagcg tcttctgcat cagttccaca
     2221 tcgggcatca gttctttcag tttcagtcga cgagcctgaa tatctgcccg cccctgcatc
     2281 ggctttttat cgccttccag atgaatattc gccgcaatgc tgccgcccgc catgccaaat
     2341 ttcagcggtt gcaggcgcag gtcagcattt ttgaggatga tatgagtaga aagatcgcta
     2401 atcggcaggc tactgccatg ctcaatgcgc cgccctttga agcgaacatc ggcatccata
     2461 acgtcccatt tatcggtttc gaagcggtca taaggcagca ctttgcccgc aggctgaacg
     2521 cttttttcgc ccttcttctg ttcagaccgt ttcgactttt ctgccccttt cccggaatca
     2581 acgccaatca acggtcccag gtccgccagc cgcaattgcc gcgactcgac atcaccttcc
     2641 agttttggtc gtggctttcc ggtggtgtag accagagaac cgtggatatc gctatcacca
     2701 attcgcccat taaaaccgcg ataatcaaag accgacgatt tttcagtgtc gattttcgct
     2761 accagccgac catccgtttc aaacggcggg gtatcgggca gcagaacgcc cgtcagttca
     2821 tagagatcac ccagtgaatc gccagaaaat ttaagccgta aatcgacacc gcccatcttc
     2881 attgggtcat tcacgacgcc atcaaaagca acacgggtgt taccagagcg gaaatcagcc
     2941 tgtaccggaa acggcgtccc ttcgccacgc agcgccagca taccgcctat ttttcccgta
     3001 cccgtgagcg gttcaccgtt atatcgtccc tgcgccttca ggccaaaaac gtaatcgccc
     3061 accttttctt tatccgcttt acctttcgat ccagtaactt cgctgaacgg cagcggcttg
     3121 cctaagggat caacaaaaat ctccagatcc gctttgctta ctttgtcatc aatggcgatc
     3181 cgcccttgat cgaaaagaat attatccagc cgaaacgacc atgccgacgg ctttgcattc
     3241 gcgtctttgt tatcatcgtt ggcaagatta aacgtccagt tattgttctt ttcagagagg
     3301 cgaatcaggc gcgcgtcggg cttttcgagc ttgatccacg gcagccagac cgttttggtc
     3361 agcagcgcca gcggggccag cgttgcctct acgcgtggca aatgcaccat cgtgacttcg
     3421 ggaatatccg gtggattgcc aagaatgatg tcttccgcgt gtacatgggg ccacggcacc
     3481 cagctgcgcc agccagtttc ttgtttttgc cgctcccaca ccacgcccag atcgccacgg
     3541 atagcgaacg gacgattcaa ctccgcagag actttctggt tgatagtcgg tttgagtcga
     3601 ttccagtcaa aagttgcaat caaaatgatc gccacgacaa tcaacaacaa gaaagcccct
     3661 gaaatcgcag cggttatttt gcctgccttg ctcatcgttc gcttcctcat attcttcctg
     3721 tgccagtcct aaagatagtc cagccaggcg gaaaatgagg cagcttatag cgccagaacc
     3781 gccgtattca gcgtttctat cggtgccggg cgtgaaagaa accagccttg tgcggcgaat
     3841 gcgggcgagt tctgaacatc acgccactct tccggcgttt ctacgccctc gacaatcacc
     3901 ccgcgacaat agcgattcat tagatgtaaa agctgagaaa agagtgtgcg tccttccggc
     3961 gactgacgca gcatcacaaa cagttctcgc gcgattttga tgtagtcata acgcacttca
     4021 cttagcgcag agaaatttgc catcccggta ccaaaatcat ccagccacag cgggccaaat
     4081 tcacacatcg aggcaaaggt tgaatctttc ggcagacgga tatgctccac cagttcgaaa
     4141 cgcagccagg gaagacgctc aatctggcgc aggatttttg gttgctgacg cagggcgatg
     4201 agcgtagggc catcaatatt gaccgatgcc agcaggccgt gctctataaa gaagtcggct
     4261 ttttgcgcca gcaaatcaat ctgctctttc acaacctcca tccgatggct gacggtgatt
     4321 tcagtaaaat agcgatccgg cggcaggcgt tgcgaagggt tcaagggatg cgtgaccacc
     4381 gttaatagct ccacggccat taaccgcccg catgtttgat agatcggctg ccaggtgtaa
     4441 gcacgctcac actgcaacca aaaacgccgt tcctgcaagc tctcgatgct tgcttcaggg
     4501 ttgcttattc gctggataac ctgccttatc atcaaagatg tcctgtgttt aaaagtgatg
     4561 ctgcccggac tcgacaaaga ttatcggcgc gtaagggcag aactttaccg cctggccggg
     4621 gtaaatttcc tcactccaca acggaatctt tcaggaatac gttctggctc aaaaaaataa
     4681 tggaacactg ttttaatatg gttgaccagc aaaccaccac agcgcaaact aacgctaatt
     4741 ttttacagat caggttcacg actatgtcca aaaagattgc cgtgattggc gaatgcatga
     4801 ttgagctttc cgagaaaggc gcggacgtta agcgcggttt cggcggcgat accctgaaca
     4861 cttccgtcta tatcgcccgt caggtcgatc ctgcggcatt aaccgttcat tacgtaacgg
     4921 cgctgggaac ggacagtttt agccagcaga tgctggacgc ctggcacggc gagaacgttg
     4981 atacttccct gacccaacgg atggaaaacc gtctgccggg cctttactac attgaaaccg
     5041 acagcaccgg cgagcgtacg ttctactact ggcggaacga agccgccgcc aaattctggc
     5101 tggagagtga gcagtctgcg gcgatttgcg aagagctggc gaatttcgat tatctctacc
     5161 tgagcgggat tagcctggcg atcttaagcc cgaccagccg cgaaaagctg ctttccctgc
     5221 tgcgcgaatg ccgcgccaac ggcggaaaag tgattttcga caataactat cgtccgcgcc
     5281 tgtgggccag caaagaagag acacagcagg tgtaccaaca aatgctggaa tgcacggata
     5341 tcgccttcct gacgctggac gacgaagacg cgctgtgggg tcaacagccg gtggaagacg
     5401 tcattgcgcg cacccataac gcgggcgtga aagaagtggt ggtgaaacgc ggggcggatt
     5461 cttgcctggt gtccattgtt ggcgaagggt tagtggatgt tccggcggtg aaactgccga
     5521 aagaaaaagt gatcgatacc accgcagctg gcgactcttt cagtgccggt tatctggcgg
     5581 tacgtctgac aggcggcagc gcggaagacg cggcgaaacg tgggcacctg accgcaagta
     5641 ccgttattca gtatcgcggc gcgattatcc cgcgtgaggc gatgccagcg taaacgcagc
     5701 atatgcaaaa ggctggataa agtgttaccc ctctatccag ctcatccata aatacctgat
     5761 ggttgtgcta actcatcacc atcaggcatt actgtgcagg tggaatatcc gtcacttcag
     5821 gatgtacgtc atccgtggcg acagaggtgg tcgcagcggc ggtagatggg gccatgattt
     5881 gatcccagac cgcctgcaac gctttcatgt taaattccgg ctcgcctttc ggctgcagca
     5941 gtattaacgc catatcattc gacaactgtt gacgcagatc ctgatttaac atctctaccg
     6001 tcaggctatt aaggaaatcc tgccgcagtt tctgatattg ctccggcgcg atatcgacaa
     6061 cctgattttg caacgaacgc atccgctgac ccatcagaat atcggtatca gctcgtgcat
     6121 aggcggcaaa cagtttctgc agctccagtt tcttttgcgc cactaacgca ttgaactctt
     6181 cttccggcag acctttatcg cgaaccttcg ccagttcacg cgccaccaga ttaaggttgc
     6241 tgttcagctt gtcgtttggt gattcgatgt tgatggcaca ctgcgcacgc agatacagca
     6301 cacggcagtc aaatccaaga ccgatgtctt tgctgttact ggcacttaac gcttgctgaa
     6361 catgccagaa cagcgcctcg cgggccaggt ccgcacgcca gtagcgcagc agtgcggctg
     6421 attcacgaat cggctgccac ggcgtatccc acatgataga taaccggtcc tgacgcaccg
     6481 cgtcagtcat aatactcacc gcttccgcac gcagcggaga aagcgtcggc accggagctg
     6541 gcgtttcacg tttgcctttc agttcgccaa acgttttgtt tatttggtcg acaaccgagc
     6601 gcgcatccac gtttcccacc accagtagcg tcattgcatc cggggtgtac catttctgat
     6661 agaaatcttt aattttttcc gcttcaacgg gttgtttcag cggatcggca ggatcgtgac
     6721 ctaacaaggt tgaccctttc agacgatagc gccaccagcc ctctttagta tcggcaggcc
     6781 aggttgccac catgtcctga ctttgcagcg cgtggttgat ggtttctggt gtgatggtca
     6841 atttgccagt ggcatttgcc agataagaga gcgcttcttt cagcaaatcg ttacggttat
     6901 tgggcaaact cagattaaac agcgtggtgt cataagagac aattaccggc ggcatcgggc
     6961 gtttagggtc gatcccctgc tgccacaatg aacgcgcctg tgctgcgtca aggccaccgc
     7021 tttgcgttag cgcaatacga gggatggcgt gactgtaacc gctctgttgt gtactttcgg
     7081 cgagcgaacc ggtattaacc agcaggcgaa tttcaacacg atcgctggga cgctgggggg
     7141 tggtcagcac ttgccactgt aaaccgttgg aaagcgtccc ctgttgccat gctggatcag
     7201 gctggagcgc atctgcctgc acatagccag cagtggccat catcagcaaa ccgcccgcta
     7261 aaagtcgaat ttttgtgccc tgcatgtgaa cccctgatca actatcctgg taataaaaaa
     7321 actgcccgtt gtcggtcagt ctaaatgacg ttaaaaacac ttcgtgttag accgcaagaa
     7381 catgaaaatg tcacggaaga agtgaaataa acctgaactc acccaggtgg taagttcagg
     7441 cacaggggtc aattatgcgc aaacacccgc actcggggaa gggagtgcgg gcataagtga
     7501 tgagattaag aggataattc gtgcgttttg ccatccggcg cacgattatt cagcacatcg
     7561 tccagttttt tgtggtccag ttctttcacc cacttagcaa cgacaatggt cgctacgccg
     7621 ttaccgacca ggttagtcag cgcacgagct tctgacataa agcggtcgat accgaggatc
     7681 agcgccagac ccgctaccgg caaatggccc accgcagaga gcgtcgccgc cagcacgata
     7741 aagccactac ccgttacccc tgccgcccct ttagaagaaa gcagcaacac gattaacagc
     7801 gtgatttggt ggacgatatc catctgactg ttagtggcct gggcgataaa caccgccgcc
     7861 attgtcaggt atatcgatgt gccatcaagg ttaaacgagt agcctgtcgg gatgaccagc
     7921 cccaccaccg atttacggca gccgagtttc tccatcttgt cgagcatacg cggcagcgcc
     7981 gactcggaag atgaagtccc cagtacaatc agcagttctt cacggatgta gcggataaat
     8041 ttgaagatac tgaaaccagt cgctttagcg attgaaccca ataccagcac cacaaacagg
     8101 atacaggtaa tgtagaaaca gataatcagc tgccccagtt gcaccagtgt gccgacgccg
     8161 tatttaccga tggtaaacgc cattgccccg aacgcaccaa taggtgccag acgcatgatc
     8221 atattgatga tgccgaagat gacctgcgag aaactttcga tgacgttaaa aatcagttgg
     8281 cctttgctgc ccagacggtg gagcgcaaaa ccaaacagta cggcaaacag cagcacctgc
     8341 agaatgttac cgctggcaaa tgcgccaatg acgctcgccg ggatgacatc cataatgaag
     8401 gcgacaatgc cctggtcttt cgcctgatcg gcgtaaaccg ctaccgcttt cgcatcaagc
     8461 gttgccggat cgacgttcat tccggcacca ggctgcacga cgttaacgat gataagacca
     8521 ataatcagcg cgatggtact gacaatttca aagtaaagca gtgcgactgc gccggtacga
     8581 ccgaccgcct tcatgctttc catgcccgca atgcccgtta cgacggtaca aaagatgaca
     8641 ggagcgatga tcatcttaat gagcttaacg aagccgtcgc caagcggttt catttgctcg
     8701 cctatttcag gatagaaatg gccaaggaga ataccaatgg ctatcgctgt caggacctga
     8761 aagtaaaggc ttttaaacag agaggttttc atagggtgtc ctttagtaaa accacaggtc
     8821 ttgtaaggtt atggggtacc tgcggcctta aaataacacc cagacaacat cacagaaatg
     8881 tacctggatc ataattgaaa caaaaaggtt aaaaagtttg agctggctcg cacaaaccag
     8941 cactttttaa agttttgtaa tcagtttggg gtagctactt ttcttccagg taactctctt
     9001 cgaagatttc aatagggagt gggcgagcaa aaaggaagcc ctgggcaata ccaacgcccg
     9061 cttttgccag ccagtcgcgt tgtgcttcag tctccacgcc ttcggcaatc atttgtaagt
     9121 tcaggctctg cgccagcatg ataattgcag caatcatgct gctatctccc ggcaagcctt
     9181 caacaaacat tttgtcgatt ttcagtacgt cgattggcaa cgatttcata tgctgcagct
     9241 gacgcagccc tgcgtagccc atgccgaaat catccagcgc cacccgaact ccggcattgc
     9301 gcagcggacg gaggattgcc accgcagcat gagggtcgtc aatacgtcgg ctttctgtca
     9361 cttccagaat cagtgttccc ggctgaatgc gatagcgggt taacagttcc agcatatccg
     9421 ccaccatatt cgggtgcatc agttgcagcg cagagaggtt taccgacaag ggcagcataa
     9481 tgccgcgctc ttgccaggct gcaagcaatc gacaggactc ttccagcacc cagtgaccga
     9541 cggtaaccat cagcccacag cactcaatgc gatcgattaa gccatccggc aggtcccaac
     9601 tgccatccgg ttgctggata cgcagtaaca cttccgcact gaccagttta ccgctggtca
     9661 tctcgacctg tggctgtaac caaatagcaa actgatgatt ttccagtgca ttaaggatat
     9721 cgctctcttc cgtcaaccgc ttctgggcgg cttccatctg ctgcggatca aagaactgaa
     9781 tctgattctt gcctttatgg cgagcggtaa atgccgcaga aatagcgcga ctgtaaagct
     9841 gttcggcggt gagatcgccg tagaacatcg ccacgccaat gctacagtgc ggacggagtt
     9901 gaatacgttc aatcggcagg cgctcgctca tgatagtgag cacttgctga cctaaggtga
     9961 ttgcgtgcca cggttcctgt acaccgttgg caatgacagc aaagtcataa ccgctaatct
    10021 gcgcgaggat catacgtggc gacagtaccg atttgagttt ttccaccagc gtcagcagca
    10081 gaatttctcg ttgcgcctct ttcagcacgc ccgcagtatc acgcagggtt tcacaggtga
    10141 tgatcatcag cgcggtggtt tgtttacgcg cgacaacctg ctccagcatc tccatcagca
    10201 aggctttgtt cggcaaatcc gacaccggga agcgcatcgc attttcgttc tgttcttcat
    10261 aatggcgctg cagcaattgc tggttgaggt tgtaactgcg caccaacata ccgatttcat
    10321 cgtcctgatg cagacgcggt aatgccagtt ggtgaccaac aagctccttg gctgggatgg
    10381 cgttaagttc gcgagcaata ttgcgtaacg gatgcaaaat caggcggtta atgcaccagc
    10441 tgatggcgac ggtcaggata agcgacaaaa gtaagtaaat ggtcactaac gttgagaggg
    10501 tgctcatcac gaacttatac atacggaagg aatccgcctg tagtaccaga taggcaattg
    10561 gctgcgggtt tgccggacgt tcgagcgagt aaacgcccag cgagatttga accggtagct
    10621 caaacaggcg agtaaccatt accggcaccg ggcgctctgg aataaaactt ttacgcagcg
    10681 cctggaactg gttaggcagc actacatcgg cacggctgac cacgcctgcc ggtttaatgc
    10741 tggcaagaat agcttccgct tcgggaatat cgcctttcaa aatagctgaa gataagggtt
    10801 cgcggacaga gcgagcaatg ctttccagtt gcgtagccgt gttatagcga ttctgctgga
    10861 ccagatggaa cagcaaaacg gtgcaaaaaa taaaaacgaa caccaggaca acggctgcca
    10921 ccattgccat ctgcttgatt gttaacgagc gacttacgcg caaattgtct ccccccggaa
    10981 atcccagatt gccgcccgag tatacccgat cgcagcggca ttaagagagg cgctatctga
    11041 aaacttacca gtcggcgtaa ggtatcagcg gctgcggcgg taaatccatg tcaccctgcc
    11101 atccggcggc ggaataacgt acgtagagca ggaaatggct gggtgcgtaa tctttcgcct
    11161 gctggatatc aattgccgtg ccaacaaacc agttggaagt aacacgtcgt tcaagtaatg
    11221 cccgcgccgt gtagccgaag ccctgactgc tgccgccatc gttggattgc cgcgcagctt
    11281 cttcctgcca gtcggtcggg atcagattca tcagcggata acgcggcatg gttttggtgc
    11341 gtgaatgcga ccacgagcca gacgcaccca gctcccacga ccagttttcc gtgcgctccc
    11401 gccacatcac cggtatggca aacgacaggt attcctgcgg actgtagtaa ccgccctgac
    11461 cgagtgagta gccactcaga tctttgtcgt aatgccagat catgttgttc aggccgattg
    11521 tgacgcggcg attgttctgg ttaatgacct tatagtaata gcccgtcatc cagcgcacgc
    11581 gccagttatc ttcgacattt ttaccggtta actggtcgcc actaagcgat gcccagacgc
    11641 cgtttgcttc acctttatcg tagctcagac ttagccccac accgtcggca cgtacgccac
    11701 cccatttttt cccggtattg ctcggggagt ctttttgccc accaaaggcc agcaaagaac
    11761 tggagatggg ccgacggtgg gcgttaacgg tgtaacccag cggcccgata tcatcgctgt
    11821 aactgatgcc gccgaccaca tccaccacgt tgaagcccat cggcgtggta ccgatatccc
    11881 agctccagac gtcatttcgc cagccgaccg ccacgctggc accggaatcc gactggctgc
    11941 ggttgccgct acagtcctgt aatgtacagg tgccccagtt gtcatcccat ttgccatcgg
    12001 cattagtgga gaaactgccg acgttcatat tgacgaaatc actgcgaaag aacatccgcc
    12061 cgtcagaata cggcgcatcc acctgcaaca tggtagtgtg cgctttcaga tcggagtaac
    12121 caccggtgcc gctcgaaccc cagtaatcgt gctcaagggt gacgttaaga tcctgctggc
    12181 gatagaggtc cgccgcatcg ctgcgcacgc cacgtttcag ccagtcatct ttctcgtcgt
    12241 tacgggtcag tcgggtaaag gtgtcgttat cctgcggacg cgtcgtagtc acaccggatg
    12301 cgaccatggc gtctttgtag gtttccagcg cctgcgttgg atcgcccgcc tgcgcttcaa
    12361 acttcgcacc atcacgcagc accatcgcgc tttccatcga cggtggctga gattttgcct
    12421 gcgggatcaa cttattaaac gtccgctgcg ctgctgcggt atcgccaagc tgcgcctgtg
    12481 ccagcgccac gcgccgctgt gtgttcagcg aggcgttatc ggtagcgggc agtttcgcca
    12541 gctggctacg tgccgccgct ttgtcaccgg cagcaatatc cacttccgtc agaccaagaa
    12601 tggcgtcggc gttagctggc tcccgcgtca ggacattctg atatgcagcg cgggcggcgg
    12661 tgtaatcacg tcgttgttgc gcccagtcag ccagcgtgag gtcaatacgc gtggaaggtg
    12721 gttgctggcg cagcatcgct tccgcttctg cctctttgcc gctttctcgc aggcggttag
    12781 cggtttccag cacctgatcg ctttgcagtc gattaaccag ctcctgaata ttgctgttcc
    12841 actgcgcacg cggcaggctg ttgatatgcg ccagcgccgc tctgtcctgg tcatgaccag
    12901 agaggtacag cccgtaagcg taaacctgct ccgggtcgtt cgacttctgc tgcgccagat
    12961 tgcgcattaa cgtatcggcc tggctgcgtt gtccggcctg ccagagatcc tgcgaaagtc
    13021 ggtaagtaat ccatacgctg ccggggtcca gcgccagtcg ttgccgctga agtgctgccg
    13081 cctgcgccca tttgccctgg ttttccagtg cctctgcctg ctgtgccaga cggtcgtttt
    13141 gcaggctgcg ttcgatatca tcaatgctac gccgctgact ggcagagagc gaggcgataa
    13201 acgcttcagc tttttctggc gattgctggc ggtaaatatt tgccagcccg cgcacggcgt
    13261 tagtgttgcc gctgtccata cgcaaggtct gctgataata acgttcggcg gcgggataat
    13321 cttttcgcgc catcgccaca tcgcccagcc ccagcactgc ataactgtcg gtgttatcga
    13381 cattacgcgc ctgctggaac aggcgttctg cccggtcagg attattggct ttcagcgcag
    13441 catcgccctg ctggatcgcc agccagtagc ggtttacttt cagcagactg ttccatttgt
    13501 cgttgttgct gctgtgcggg tccagtgcga gggctttttc cagattcgcc actgcattgg
    13561 cgcgatcgcc tttctgagaa tacgcctggc ccagcgcccc cagagcttca ctgtctttcg
    13621 ggttcgcccg caccgcctgt tgtagttcgg gaatggcttt acccgccata ccagagtcca
    13681 ccgccgctaa accttgcgca cgagcgcgga aagcaggatc ggccagctgt ttttgctgtt
    13741 ctgccagttg cgattgcgca gccgccacgc tatcgccatc actaaagatc gagagatatt
    13801 ttttcagcgc cgacacactg gcatcactga cgggcatgtc tttaatctgc ccgtaccaga
    13861 ttttagaggc cccttcgcgc ccggcgttcg attttgccat ctgttccagg acggcaaaac
    13921 cttcgtcacg gcgatcgcta ctaaacagca atagcgccag attgttttgc aggcccgtat
    13981 tgcccggtgc atccgcattg atgcgtttta actgattaat cgcttcgcca cggcgagccg
    14041 gaattttcgc caccgtactc cagtactcga cagcaatgtc accttccggc ggcgcaccgt
    14101 tgaacagttt gttgtaactc gccacagctt cttctgcatg accggtcgtc gcctgcaatc
    14161 gtgcctgttg cagtgcctga cgaccatccg gcgtggaaag tagcatcgta gtccgcgacg
    14221 atttatacgc atttgaactc ggcgctaact gcgacagccg atcgagctgt ttttgcgcgc
    14281 catcaatatc gccctgacgt aacaaagaac ggaaacgggc ggcaacgacg tccgggttat
    14341 tcggatcaat aagttccagc cgatataacg actgttgcac cagatcttca cgatgggtcg
    14401 cttcgcctaa ccgaacttgc tccagcaact gttgctgagc ggttggtgct gcctcgacca
    14461 tcggcatgac ggccagaccg agggaaagcg tgaatatgtt tagtgtgaat ttgcgcattc
    14521 ctggccccag tcaggtaata actcaccttt tgtcgagaag cggaaacggt gttgatccca
    14581 gccttgtcca aacagggtca gcacatagtt gtaataggca tcgctgccgg gaaagttatc
    14641 ggccacgcgc tggcgctgaa cggcctgcgc atcgcggttt tgtaaaaagg gcagcatggc
    14701 ggcagaaaaa ccgactggtc ctttaccctg cgctttcccc gtagccacat ccactttttc
    14761 cggcggataa ccgtttttct cagtgaatgt cgccatcggt ttaaaccggt tgagcatccg
    14821 cgctttttgc ggatcgctgt caggcatcat gcctacccac atgtaaacgc ggatagcgtc
    14881 gtagctgctg atcaatgttt tttcggcttt tagctgccag cctttgtctt tctcatagcg
    14941 cacccagtct ggcgaaaaac ctttcggggc ggtttccagc aataaacgtt gattggtttc
    15001 gcgcagcgtg gtccacggcg cgccaaagcg ggtgaaatac tgcgccagcg tcggcggcag
    15061 gtagctgggg ttaaaacgcc agctgttatc ctcagcaaaa cccactttgc ccggtaacaa
    15121 catggaaccc agcccaggca ccgtcaccac ttcctcccgc gcgatacgtt ttagcaacgc
    15181 gctgccgatg tcggtataac gctgctcttt ccacaaacgc cccgcctcca gcaacgacca
    15241 ggccatccag acatcaccat cggaggccga attgctgtcc agcacttccc acttactgtt
    15301 ctctttcttg ccccacagcc aggcgggcaa acgttctttt aaagaaccct gagcgagatt
    15361 gttctgcgtc cagtcgagaa tattatcgaa agctgcacgg tcgttagccg ccagggcaga
    15421 gaacatgccg taactttgcc cttcggaggt ggtgattttg cgcgcgtcgc tggggtcgat
    15481 gacgcgccct tcctgactga tgtaatcctt tttaaactgc tcccaggcag gccaggtaca
    15541 ggctgcctga acactaaagg cagccagcag cagcatcgtc acgattccac tacgcaacac
    15601 attcatcttc aattactcgt tatccgggtt aagacgacga cgactaataa ttcgcagcag
    15661 acgccacagt acccatgcca gcaatatcac actgatagcc gccagcaccg ccagcagaat
    15721 cggatggttt gccagcgcat accacacgcg ctcgaaccac ggcagatgac ctacgtaata
    15781 aacgtcgcca acacgtaggc tgttgatacc ggactcgcgg atcaccgcga ccgaaccgaa
    15841 catggtggcg cgtttgccgc tatcgttcac cgcatcgtta agcatttcat aaccgcgtgg
    15901 gctatctgcc aacagcgcaa tcacgctgcg ctggtcgtta tacggcgact ggaagccaat
    15961 caccgccgcc atcgcaccgg aagaagtcag cgttgaccgg gtttctgccg cgcgatcgct
    16021 ctcgtccggc acaatgccgg ggaacggggt ctggcgcatc ggtgttttca cccagctttc
    16081 ggtcgcctgc accaata
//
LOCUS       DBBGLQ010000098        16074 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.098, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000098 DBBGLQ010000000
VERSION     DBBGLQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.098"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..846
                     /locus_tag="ACIYSB_004106"
     CDS             <1..846
                     /locus_tag="ACIYSB_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HGC5431038.1"
                     /translation="TAFKELYDADVSPALISKVTDAVMEQVVEWQNRPLDAVYPIVYL
                     DCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTELKNRGL
                     NDILIACVDGLKGFPEAINAVYPETQIQLCIVHMVRNSLRFVAWKDYKAVTRDLKQVY
                     RAATEEGAFQALDAFGKECDALYPQISRSWRANWPNVATFFVYPEAIRKVIYTTNAIE
                     SLNSVIRHAIKKRKVFQTDESVKKVVWLAIQSASQKWTMPLQDWRIAMSRFIIKFGDR
                     LDGHF"
     gene            complement(856..2375)
                     /locus_tag="ACIYSB_004107"
                     /pseudo
     CDS             complement(856..2375)
                     /locus_tag="ACIYSB_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2485..2832)
                     /gene="tnpB"
                     /locus_tag="ACIYSB_004108"
     CDS             complement(2485..2832)
                     /gene="tnpB"
                     /locus_tag="ACIYSB_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGC5431039.1"
                     /translation="MITLPAGSRIWLVAGITDMRNGFNGLAAKVQNTLKDDPISGHLF
                     IFRGRRGRLVKLLWADSGGLCIYMKRLERGTFIWPVAREGKVYLTPAQLAMLLEGIVW
                     MQPGRSKSNSLRI"
     gene            complement(2829..3239)
                     /locus_tag="ACIYSB_004109"
     CDS             complement(2829..3239)
                     /locus_tag="ACIYSB_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5431040.1"
                     /translation="MESETRAWRTEPRRVYSSEFKLRLVELATRPDANVADIARTHGV
                     NDNVLFKWIRLWRKEGRVFRRLPAPVPSVSPPAAFPVEIVPDSTTRQSPALSGQGTGS
                     TCSLTLRHGTLTLDNPDPEIQLRIVRELAGRSTV"
     gene            3395..3616
                     /locus_tag="ACIYSB_004110"
                     /pseudo
     CDS             3395..3616
                     /locus_tag="ACIYSB_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611680.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3869..4370
                     /locus_tag="ACIYSB_004111"
                     /pseudo
     CDS             3869..4370
                     /locus_tag="ACIYSB_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            5042..5467
                     /locus_tag="ACIYSB_004112"
     CDS             5042..5467
                     /locus_tag="ACIYSB_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431041.1"
                     /translation="MSFNTTHEKSEIYRLILRESELITAWVKSGDTPSAVYGKLRDKN
                     PDIIFSINGFLYNLRNFNYALYETATKNKSKTRLIILNHYDDIASAIRAGHTLKGVYK
                     LVCPHITYNCFITQLRKTYPDLHSQGKANRSNKNRIIAN"
     gene            complement(6637..7458)
                     /gene="iolB"
                     /locus_tag="ACIYSB_004113"
     CDS             complement(6637..7458)
                     /gene="iolB"
                     /locus_tag="ACIYSB_004113"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HGC5431042.1"
                     /translation="MSRLLSRWQQPNAEGRTQSVTPESAGWGYVGFEAYELEEGQQLT
                     LPAVSEERCLVLVAGRAFISTPSAQFTDIGERMSPFERIKPWAVYVTPQEAVQVKALT
                     KLELAVCTAPGKGTYPTRLIAPQDIDGEARGNGHNQRYVHNILPEDKPADSLLVVEVW
                     TNEGCTSSYPSHKHDTNNPPQESYLEETYYHRLNPEQGFCMQRVYTDDRTLDECMAVY
                     NRDVVMVPKGYHPVATMAGYDSYYLNVMAGPVRKWIFTWEKEHSWINTEYSAISL"
     gene            complement(7468..8358)
                     /gene="iolE"
                     /locus_tag="ACIYSB_004114"
     CDS             complement(7468..8358)
                     /gene="iolE"
                     /locus_tag="ACIYSB_004114"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010433880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HGC5431043.1"
                     /translation="MSVQLGINPLTWTNDDLPSLGAETSLETCLSEGKEAGFAGFELG
                     NKFPREARLLGPILQRHDLQLVSGWYSGRLLERSVEDEIAAVQSHLTLLRELGAKVLV
                     FAEVSGCIHGEQQTPVHLRPRFPQECWKEYGEKLTVFARYTQQQGVQIAYHHHMGTVI
                     ESAQDVDNLMLHTGEEVGLLLDTGHLTFAGADPLAVAQRWASRINHVHCKDVRADVLA
                     DVKNRKTSFLDAVLSGVFTVPGDGCVDYPPIMQLLKTQDYHGWLVVEAEQDPAIAHPL
                     TYARLGYNNLSRLARDAGLI"
     gene            complement(8370..10274)
                     /gene="iolC"
                     /locus_tag="ACIYSB_004115"
     CDS             complement(8370..10274)
                     /gene="iolC"
                     /locus_tag="ACIYSB_004115"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HGC5431044.1"
                     /translation="MEKQFDVICMGRVAVDLYSQQIGARLEDVSSFAKYLGGSSGNVA
                     YGTARQGLRSSMLARVGDEHMGRFLREELNQVGCDTSHLITDKERLTALVLLGIKDRD
                     TFPLIFYRDNCADMAITASDVDESYIASARCIAITGTHLSHPQTREAVLTALNYARRH
                     GVRTVLDIDYRPVLWGLTSLGDGETRFIVAEQVTRELQEVLHLFDVVVGTEEEFHIAG
                     GSTDTLQALAQVRHVSKATLVCKRGALGCSVYTDAIPPRLDDGLTVTGVRVEVLNVLG
                     AGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPSKIELDDYLSRAAL
                     VPRPDIDPRLNHLHRVTTRRREWPELCVMAFDHRSQLEDMALQCGASLKRIPALKQLI
                     LQASREAASSAGLEGKAGLLCDGTFGQDALNAITGEGWWIGRPIELPGSRPLEMEHGN
                     IGTQLISWPQEHVVKCLVFFHPEDAHGLRLEQEQKIAEVYHACCQSGHELLLEVILPA
                     TMPRSDELYLRAISRFYNLGIYPDWWKLPPLSAEGWTALSEIIARRDPHCRGVVILGL
                     DAPAEQLRADFKAAAGQALVKGFAVGRTLFGDASRAWLKHDIDDAQLVARIRDNYLQL
                     IAWWRERGHA"
     gene            complement(10287..11420)
                     /locus_tag="ACIYSB_004116"
     CDS             complement(10287..11420)
                     /locus_tag="ACIYSB_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGC5431045.1"
                     /translation="MKEVRIGLIGTGYIGKAHAIAYAQAPTVFNLRGKLVREMVAEVN
                     PALAAERAQAFGFNRSTGDWRVLVADPAIDVVDICSPNHLHKEMALEAIRHGKHVYSE
                     KPLALNAHDAREMVDAAKRAGVKTLVGFNYMKNPTAQLAKEIIACGEIGEVIHFYGTH
                     NEDYMADPLSPIHWHCFKETAGLGALGDLAAHIVNMAQYLVGEIEQVCGDLKIVVPAR
                     PAKAGSSEMVTVENEDQAHAMVRFAGGAQGVIETSRVACGRKMGLSYVITGTKGAISF
                     TQERMAEIKLYLHDDPVNRQGFQTLLVGPAHPDYGAFCMGAGHGIGFNDQKTVEVRDL
                     VDGIATDAPMWPDFEEGWKVSRVLDAIALSHQQGLWLNVNDIV"
     gene            complement(11429..12457)
                     /locus_tag="ACIYSB_004117"
     CDS             complement(11429..12457)
                     /locus_tag="ACIYSB_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5431046.1"
                     /translation="MTQMIPKTQVPVKRAGRIDPIAFFERFGVLIFMILLLIFFQSQN
                     SNFFSERNIFNILTEVSIYGIMAVGMTFVILTAGIDLSVGSILAVCAMTAAYVIKGDN
                     FTTVDPNAWGGMSWLIGLGICLTMGTAIGFLHGLGVTRLRLPPFIVTLGGMTIWRGLT
                     LVINDGAPIAGFDQGYRWWGRGELLGISIPIWIFAIVAIGGYLALHKTRWGRFVYAIG
                     GNPEAARLAGVNVKRVLVSVYVLIGCLAGLAGFILSARLGSAEAVAGISFELRVIASV
                     VIGGTSLMGGYGRIGGTIIGSIIMGILINGLVLMNVSAYYQQIITGLIIVLAVAFDTY
                     AKSRRGAL"
     gene            complement(12469..14016)
                     /locus_tag="ACIYSB_004118"
     CDS             complement(12469..14016)
                     /locus_tag="ACIYSB_004118"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGC5431047.1"
                     /translation="MSQPLLKVTDLAKSFSGVWALSSAQLTVGTGEIHALLGENGAGK
                     STLLKALAGAQPQTRGEIWFNGETLPVDDSPVERQNKGIITIYQEFNLLPNMTVAENM
                     FLGREPRKRNLIVDEKAVNQEAQVILDYLQLNVAPTTPVARLSVAQQQMVEIARALTL
                     NARLIIMDEPSAALSDSEVESLHRVVRELKNRGVSIIYVTHRLHEVFQLCDRFTVFQD
                     GRFTGTGAVTETNVEQLIRLMVGRDVAFNRRPASETHHEDKPVRLSVKGLSREKPPLD
                     PHGIALHDISFHVHAGEVLGIAGLVGAGRTEVARCLFGADAFTTGSFELDGVPYQPRD
                     PLYALEQGLALVPEDRKKEGAVLGLSIRYNLSLSCLSSLLQWRWFVNTRKEDDLIESY
                     RQALQIKMVNSTQEVRKLSGGNQQKVILARCMALNPRVLIVDEPTRGIDVGTKSEVHQ
                     VLFDMAKKGVAVIVISSDLPEVMAVSDRIITLSEGRLTGEIHGDDANEERLMTMMAIN
                     HNALNAA"
     gene            complement(14068..14997)
                     /locus_tag="ACIYSB_004119"
     CDS             complement(14068..14997)
                     /locus_tag="ACIYSB_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGC5431048.1"
                     /translation="MKKLILAALIAMTSGAAIAENEQIVFSTPNLAMPFEVHMQRTAV
                     KAAKEMGVKLQVLDGQGSSPKQVADLENAITRGAQGFIVSPNDVNAVSSAVDEIQDTK
                     LPVVTLDRSVDSQKKVPHFGANNYKGGQAIGDFVKTKFPNGAEIILLTGQPGSSSNIE
                     RTKGIRDSLKAGGDMYKIVADQTGNWMRSEGMRIVESVLPSLPKRPQVILSANDDMAL
                     GAIEALQSQGVKPGEILVTGFDAVPEALARVRDGWLAVTADQRPGFAVTTAMSQLVAN
                     VREKKTITGADYPPTLITKENLQQAERIAEAGN"
     gene            complement(15029..16015)
                     /gene="iolG"
                     /locus_tag="ACIYSB_004120"
     CDS             complement(15029..16015)
                     /gene="iolG"
                     /locus_tag="ACIYSB_004120"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HGC5431049.1"
                     /translation="MFNIALLGAGRIGQVHAANIASHSATTLWSVVDPNQEFATRLAT
                     QYQARQQSLDEAMTDPNVHAVLIASATDTHADLIEMAARYGKAIFCEKPVHLDLARVR
                     DCLKVVKEYDVPLFIGFNRRFDPQFRRVKTDTQAGRIGKPESLLIISRDPSPPPAEYV
                     RVSGGMFRDMTIHDFDMARFIMGEEPVSVYAQGSNLVEPAIGGAGDIDTAFIVLKYAS
                     GAMATIVNSRRSSYGYDQRLELHGSEGLLCAGNILENQVQHYGKQGCTSALPEHFFLQ
                     RYKSAYAAEWEHFVAVLRGEAVPDCSGDDGERALYLADKALESLRCQREIVL"
BASE COUNT         3869 a         4493 c         4050 g         3662 t
ORIGIN      
        1 actgcgttca aagaactgta tgacgcagat gtttcaccgg cactgatatc aaaggtcacc
       61 gatgccgtga tggagcaggt tgtagaatgg caaaacagac cactggatgc tgtttacccc
      121 attgtttatc ttgactgtat cgtcctgaaa gttcggcagg acagtcgcgt catcaacaaa
      181 tcggtgttcc tggcactggg catcaatatc gaaggtcaga aggaactgct gggtatgtgg
      241 ctggccgaaa atgaaggtgc aaagttctgg ctgaatgtac tgacggaact gaaaaatcgc
      301 ggcctgaacg atatccttat cgcctgcgtg gacggcctga aaggcttccc ggaagcgatt
      361 aatgccgtct acccggagac gcagatccag ttgtgcatag tacacatggt acgcaacagc
      421 ctgcggttcg tggcgtggaa agactataaa gcggtaaccc gtgacctgaa acaggtttac
      481 cgcgcagcca cggaagaagg cgcgtttcag gcgctggatg cgttcgggaa ggaatgtgat
      541 gcactgtacc cgcagataag tcgcagttgg cgggcaaact ggccgaatgt tgccaccttc
      601 ttcgtgtacc cggaggcgat ccgcaaggtt atctacacca ctaacgcaat cgaatcgctg
      661 aatagcgtca tcagacatgc gataaagaaa cggaaagtgt tccagacaga tgaatcggtg
      721 aaaaaagtgg tgtggctggc tatccagtca gcctcacaga aatggacgat gccacttcag
      781 gactggcgga tagccatgag tcgctttatt atcaaattcg gtgaccgcct tgacggtcac
      841 ttctgagaaa ggcatttaca cagaatggta aactgggtcg atacagctga ttacctcacg
      901 caaccagctt tccggatcca ctccattcag ccggcatgtc gccagcaggc tgtacaggat
      961 cgctgcgctc tgacctcccg tgtctgagcc gaagaataga cagttgcgtc gccatagggc
     1021 gacacaacgc agtgtgtttt ctgcgatatt attgtccagt tccacacgcc cgtcctcaca
     1081 acagagcgac tgcgcttcac ggtggttcag catatagctg aacgctttcc ccagctctgc
     1141 atgcaccgac aacgtctcca tctgttctgt cagccacgcg aacaacgcct tcatctgcgg
     1201 gacgcttttc tcctgccgtt ctgccagccg tttctctgcc ggttgacccc ggattgtcgc
     1261 ttccaccgca tacagcccgg ctatacgctt cagcgcctcc gttgtcatcg ctgtcggccg
     1321 gcgaacatgc tcatcatgga tctttctcct tgcatgcgcc atgcaggcgg cttccctgat
     1381 cagtcctgac tcataaagcg catcgtaacc ggcaaacgca tctgcctgca ggataccttt
     1441 ccactccgcc agatgcccct gcgggtgaca gccccgacga tctgtactgt aagcgaacca
     1501 cgccgctggc ggagccgctg aaccgctgtt ccggtcatcg cgcacataca cccacagccg
     1561 gcccgtccgg gttttaccgc tccccggctc cagcactttc accggggtat cgtccgtgtg
     1621 ctgtttgctg ctctccatca cgtagcggtt cagcgcatcg tgcaggggac tgagtcgttc
     1681 tgcggtcatc gccacccacc ggaccagcgt gtttcggctc agttccacac cctggcggct
     1741 gaagatgacg gactgacgat gcagcggggt atggtcacag tatttactca ccaggatgcg
     1801 ggccagcagg cccgcaccgg ccacactgcg ggtcagaggt ttcgggagga gtggggcctg
     1861 gactatcaca tcgcagcggc tgcaggccag cttcgggcgc accgtttcga tgaccttaaa
     1921 ggcgctgcgg attatctcca gttgctctga gacggtttca ctcatcacct tcaggtcgcc
     1981 gccgcatttc gggcagacag tcactgtcgg ggtgatgcgc cgggtttcac gtggcaggtc
     2041 tgccggcagt ttctgacgac gggagcggcg tgcaggccgg tcctgtgggg cgaccgccac
     2101 cgggggctct gcctgctcaa ccgtactgac agtgggctca tcgtcctgca gacagcgttt
     2161 tgccgctgca agacggtttt ccagctcagc cagccgcttc tctgcctggc ggattttatt
     2221 ttccagttta ttgcggcttt tctctgaact ctgcccgaac agcatccgcc gcagcttatc
     2281 aacttatcaa gctgtgcctt cagccgttcg atttcctgtt cgtagtcggc aacctgtccg
     2341 gcgtactggc gcagccgggc ctgctgcttt ttcatggtaa catttagctg ttacacagca
     2401 acctgctgct gacgcaacag ggctttcagc tggtcgatat tatcaggaag cgggatattt
     2461 ttcatcctgc ccaggtaaag ggattcagat ccgcagacta ttacttttcg accttcccgg
     2521 ttgcatccag actataccct ccagcagcat ggcaagctgt gccggggtca gatacacctt
     2581 tccctcccgg gcgaccggcc agatgaacgt tccccgttca agtcttttca tatagatgca
     2641 gagccctccg ctgtctgccc acaggagttt taccagcctg cccctgcgtc cccggaagat
     2701 gaagagatgg ccggagatcg ggtcatcctt cagggtgttc tggacctttg cggccagacc
     2761 gttgaagccg tttctcatgt cggtgatgcc ggccacgagc cagatacggc tgccagccgg
     2821 aagcgtaatc atactgtgct cctgccggcg agttcgcgga cgatccggag ctgtatctcc
     2881 gggtcaggat tgtcgagtgt gagggtgccg tggcgaagcg tcagcgagca ggttgacccc
     2941 gttccctgac cagacagagc cggagactgc cgggtggtgg agtcaggtac aatttcgacc
     3001 ggaaatgccg caggagggga tacagatgga acgggagctg gcagccggcg gaatacccgg
     3061 ccttctttac gccacagacg gatccattta aaaaggacgt tatcgttgac cccgtgcgta
     3121 cgggcaatat ccgcaacatt ggcgtccggt cgggtagcca gttcgaccag cctgagtttg
     3181 aattcgctgg aataaacgcg gcggggttcg gtccgccagg cccgggtttc agattccata
     3241 acttagatgt ccgtctgttt cagatgggcg tctaagttag cgttttcact cgactgcctg
     3301 aagacgtctc tgacgggtcg cttacgtaaa atcaaccacg ggaagctcaa cggtgaagta
     3361 ctttaccagt tgatcaagcc aggcaagagg tcagtatact gaactgaaat atcaacaact
     3421 tctctggtgt tacaggataa agcaaagtat cagccgatag ggaaactgct gggataacag
     3481 cccaatggaa cgcatcttca ggagtctaaa gaacgaatgg ttaccagtgg tggggtacgt
     3541 aagcttcagc gatgcagttc aagtcataac ggactatatc gttgggtatt atagtgcact
     3601 caggcctcac gaataaaatg gcaggttact accaaacgaa ttggaaaata gatactggaa
     3661 aaaaatttaa aatcgtgatc attttttgtt gaccactaca aacatttatg tttttttatg
     3721 cgaatgaagt tatccttcct ttctgcctgg ttgtctcatt catacagata tctggcagac
     3781 ataacttgtt gtgaaaagta gatgcttacc gcatgtctga tgcacttttg tataaaggca
     3841 aagtatttca gcaaatggaa atgattatat gaacaacaca gactcattag aaaaaataat
     3901 caaacatcaa caaaacaacg atccaaaatc cccctgccag gagcatctgt tgatacaact
     3961 ctgtatccgc gtaaacaaaa aaaatacagg ataatatatc gagtttactc agtggatatg
     4021 gaataaatta ttcaacatat atggttctta ccatattatc cacagcagat aatcattgtc
     4081 tttcgccctc aaagataagc agagaactcc agtttaccag aacaaacatt acccgcatta
     4141 cagatttttt agaaaaagca gggtatatag aaagaactga tagcagagag gatcgccgtg
     4201 ctaaaaaaat cagtctgaca tcagatggtc tgtcctttat tcagaacgtc aatattgcac
     4261 aaagtctgca cctgaaagaa atctggagcc cgctgaccca tgtagagtgt gaactgtttg
     4321 aagacattaa taaaaaatta ctgacgcata ttgctgatgt gtgtccctaa agcatgaaga
     4381 gtttgaggaa actgaagagc ttcaagcaat gaatcatata ataagcaggc ataagagcga
     4441 cataactaca ggcggaaaca gcgatacaag aaaactactg gtttaggtca caatgcagca
     4501 aaatgacaaa ctcatacgac ggttcttgtg aatgatgcgg tgcctaaccc gcaaatatca
     4561 gcatgattca ccgtagaact tactgataca agataacgta atgattgagc ttatctcata
     4621 tggggtaacg cgcaataaaa atagttgaaa cattgtttat aattgttcaa tctatcttat
     4681 ttttattaat atagttgagc ttctcaacac attactttaa agttctacac taaagagaac
     4741 aaaattatca caacggatat ggagatactt tcaggccagt caaaccggtt taactgatat
     4801 ggttgaatac acgcttgact atgaatggtt tatggtattc agtcccgata actatggggc
     4861 cagttttgtg tagctttcaa ctttagttgt tttcactcat catagatgat gcaataaaaa
     4921 catcattttc tcacgcattg catattgctg gtatatccat atcgcatgta gcacagagtg
     4981 ttgactaaat tatagaaata tgatttttta tctcattgac aaagaagtta aggaccgaga
     5041 gatgagtttt aataccacac acgagaaatc tgaaatctat cgtctgatcc ttagagaaag
     5101 tgaactcatt acagcatggg taaaatctgg agacacgccg tcagcagttt acggaaaact
     5161 acgtgataaa aacccggata taatcttttc gataaatggc tttttatata acctacgtaa
     5221 ctttaactat gcactatatg aaacagccac aaagaacaaa agcaaaacaa ggttaataat
     5281 actcaaccat tatgatgata ttgcatcagc tatccgtgct ggtcatacgc ttaaaggtgt
     5341 ttacaaactc gtatgcccac atataacata taactgtttt attacacaac tcaggaaaac
     5401 ctatcctgac ctgcattctc agggtaaagc gaatcgcagc aacaaaaaca gaataatagc
     5461 taactgatga tatataaaat gttttacact atatctttta ttgtaactaa aattactttg
     5521 atcctacaaa aagaaaggta agaaggggtt taaaagatca tcacgagaca actgttctag
     5581 ttttttaagc cccccattaa tttaaaaaac agataccaag catgaataag attatataaa
     5641 tttcattaca tacaattcat tactgtccgg acaaacattg ttgtttataa aatataacac
     5701 accaattgta aagctgtgag gtcatgctac ttttaacaga atttttaaac aagagggaat
     5761 gggagaccat taataatgca agaaacaaaa atcaaccgca aatctaactg tacaaactgc
     5821 aatattcccg gatgtaagca aaccccgttt tgcaagtact gtcgtagtgt caaagatata
     5881 tccggagcta gacgctcttt atatctccat gtagcaatat tcatcattat tattgtactt
     5941 actattattt cagtactttg acaagccttt ttgcaccaac tgatgtacac aatcagattt
     6001 cagcctgtcc gtaataatta tatttgatat tacattaacg ctctttaact gtaggacagc
     6061 acagcgtaaa ttgttgaata gttcatttaa aaaatcataa ggataaaaaa ccaacactta
     6121 aaacattgtt ctttttcaag aacacatagt taaataaaat aacaaaccac taccaacttt
     6181 aaaacattaa aaaaaactgt gagagattac tttaaaaaga gttcatagct ttttaataaa
     6241 ccatataaaa taaacaatac atatgttacc cccccagcaa aaacgtgaca aaaacataac
     6301 accataagca ggtaccatat aaccgcacac caccataacg aattggtcac tggttcatga
     6361 caaaccgttc ataattgcta aaaatattaa tacaccatca tatagacttt catgtctaat
     6421 gaaaaaaata tttatactcc agaatagcca caacctttat aaaattcagc tcattagaat
     6481 ataaatgcat atgccaatga acagaataca ttctcatcct tattgaggaa catactgcct
     6541 gagagaaaaa tcatcactca gactttgaaa taacattgtc gagttcatat tatacaaaag
     6601 gggagtcatt cccctttttt agcttaggta ctcagtttat aaagaaatgg cagaatactc
     6661 tgtgtttatc catgagtgtt ccttttccca ggtgaatatc catttacgta ctggtccggc
     6721 catcacgtta aggtaataac tgtcataccc ggccatcgtc gccaccgggt ggtacccttt
     6781 cggcaccatg accacgtcac ggttatagac cgccatacat tcatcaagtg tgcggtcatc
     6841 ggtatacacc cgctgcatgc agaacccctg ttccgggttg aggcgatggt aataggtctc
     6901 ctccagatag ctctcctgag gcgggttatt cgtatcgtgt ttatggctcg ggtacgaact
     6961 ggtacagcct tcgttggtcc agacctcgac gaccagcagg ctgtcagcgg gcttgtcttc
     7021 cggcaaaata ttatgcacat aacgctggtt gtggccgtta ccgcgcgctt cgccatcgat
     7081 atcctgaggt gcaatcaacc gcgtcgggta tgtccctttt cccggagcgg tacagactgc
     7141 cagctccagt ttcgtcagcg ccttcacctg cactgcctcc tgcggggtga cgtaaaccgc
     7201 ccaaggtttg atgcgctcga acgggctcat ccgctccccg atatcagtaa actgtgcaga
     7261 aggagtgctg atgaaagcgc gcccggccac aagcaccaga cagcgctctt cactgacagc
     7321 gggcagcgtc agttgctgcc cttcctcaag ttcataggcc tcaaagccaa catatcccca
     7381 tccggcgctt tccggcgtga cagactgagt ccgcccctca gcgttcggct gttgccagcg
     7441 tgataacaga cgtgacatgt cacctcctca gataagcccg gcgtcgcgcg ccaggcggct
     7501 cagattgtta tatcccagac gggcgtaggt cagcggatgg gcgatcgccg ggtcctgttc
     7561 agcctccacc accagccaac cgtgataatc ctgcgttttc agcagttgca taatcggcgg
     7621 ataatccacg cagccgtcac ccggcacggt aaacacgccg ctcagcaccg cgtcgaggaa
     7681 gctggtttta cggtttttga cgtccgccag cacgtcggcg cgcacatctt tgcagtgaac
     7741 atgattgatg cgcgatgccc atcgctgtgc caccgccagc ggatcggccc cggcaaaggt
     7801 caggtggccg gtatccagca gtaaaccaac ctcctcaccc gtgtggagca tcaggttgtc
     7861 cacgtcttga gcggactcaa tcaccgtgcc catatgatgg tggtaagcaa tctgtacccc
     7921 ctgttgctgc gtatagcggg cgaacacggt gagcttctca ccatactctt tccagcattc
     7981 ctgcgggaaa cgtgggcgca ggtgaaccgg cgtctgctgc tcaccgtgaa tgcagccact
     8041 cacttccgca aaaaccagta cttttgcacc cagctctcgc agcagcgtca gatgcgactg
     8101 cacagcagca atctcgtcct ctacactgcg ttccaacagg cggccggaat accagccgga
     8161 gaccaactgc aggtcgtggc gctgcaaaat gggcccgagc aggcgcgctt ctcgcgggaa
     8221 tttattgccc agttcgaagc cagcaaaacc ggcctctttc ccttcactca ggcaggtctc
     8281 cagagatgtc tccgcgccca gtgaaggcag atcatcgttc gtccacgtca gcgggttgat
     8341 gcccagttgt acactcattg tcgtctccat tatgcgtgtc cgcgctcgcg ccaccaggcg
     8401 ataagctgca ggtagttgtc ccggatgcgc gccaccagct gcgcatcgtc aatatcgtgt
     8461 ttcagccatg cgcgggaggc atcgccaaac agcgtgcgcc ccacggcgaa gcctttcacc
     8521 agcgcctgcc ccgcggcagc tttgaagtcg gcacgcagct gttccgccgg agcatccaga
     8581 ccgaggatca ccacgccgcg gcagtgcggg tcccggcgcg cgataatctc gctcagtgcc
     8641 gtccagcctt cggcggagag cggcggcagt ttccaccagt cggggtaaat gccaaggttg
     8701 tagaagcggg aaatggcacg cagataaagt tcgtcactgc gcggcatagt ggcgggtaat
     8761 atcacctcca gcagcagttc atgtccggac tgacagcagg cgtggtacac ctcggcgatt
     8821 ttctgctcct gctccagacg caagccgtgg gcatcttccg gatggaaaaa gaccaggcac
     8881 ttcaccacat gctcctgcgg ccaactgata agctgggtac caatgttacc gtgctccatc
     8941 tccagcggac gcgagcctgg cagttcaatg ggacgaccaa tccaccaccc ttcgccggta
     9001 atggcgttca gcgcatcctg accaaaggtg ccatcgcaca gcaggccggc tttcccctcc
     9061 agcccggcag agcttgccgc ctctcggctg gcctgcagga tcagctgttt cagcgccgga
     9121 atacgcttaa gcgacgcgcc gcactgcagc gccatatctt ccagttgact gcggtgatcg
     9181 aacgccatca cgcacagctc cggccattcg cggcggcggg tggtcacccg gtgcagatgg
     9241 ttaagacgcg ggtcaatgtc cgggcgcgga acgagcgcgg cgcgggagag gtaatcatcc
     9301 agctcaattt tgctcggcat cgccggtgca cagccgtggc gcgagaccac cagcgcccca
     9361 caggcgttgg cgtagcggca cgcctgctcc cagccctcgt cgttcaggta accgcgcagc
     9421 aggccagaca taaacgcatc tcccgcgcca aggacgttaa gcacctccac acgcacgccg
     9481 gtcaccgtca ggccgtcatc cagccgaggt ggaatggcat cggtatacac cgaacagccc
     9541 agcgcgccgc gcttgcaaac cagtgtcgcc ttgctgacgt ggcgcacctg tgccagcgcc
     9601 tgcaacgtat cggtgctgcc ccccgcaatg tgaaactcct cttcggtgcc gacaacgacg
     9661 tcgaaaaggt gtagcacctc ctgaagttca cgggtaacct gttcggcaac gataaaacgc
     9721 gtttcgccgt ctcctagaga ggttagtccc cacagcaccg gacggtagtc gatgtccagc
     9781 accgtgcgca cgccgtgacg gcgggcgtaa ttcagcgccg tcagcaccgc ctcgcgggtc
     9841 tgcgggtgag aaaggtgagt cccggtgatg gcaatacacc gtgcggaggc aatgtagctt
     9901 tcgtccacat cgctggcagt aatcgccata tccgcgcagt tatcgcgata aaaaatcaga
     9961 ggaaaggtgt cccggtcttt aatgccgagt agcaccagcg ccgtgaggcg ttctttatcg
    10021 gtgatcagat ggctggtatc gcagcccacc tggtttaact cttcccgcag gaagcgcccc
    10081 atatgttcat cgccaacgcg cgccagcatt gacgagcgta agccctgacg cgcggtaccg
    10141 tacgccacgt tgccggacga gccgccgaga tatttggcga agctcgacac atcctccagg
    10201 cgggcaccaa tttgctggct gtagaggtct accgccacgc ggcccatgca tatcacatca
    10261 aactgctttt ccacgttgaa ccctcttcag acaatgtcat tcacgttcag ccagaggccc
    10321 tgctggtgtg agagtgcgat ggcatccagc acgcgcgaga ccttccagcc ctcttcgaag
    10381 tccggccaca tcggtgcgtc ggtggcaatg ccgtctacca gatcacgcac ttccaccgtt
    10441 ttttgatcgt taaagccgat accatgacct gcgcccatgc aaaacgcgcc gtaatccggg
    10501 tgtgccggac cgaccaacag tgtctggaac ccctgacggt tcaccggatc atcatgcaga
    10561 tagagcttta tctctgccat ccgctcttgg gtaaaactga tcgctccttt ggtaccagta
    10621 atgacgtatg acagccccat cttgcggccg caggcgacgc gggaagtttc aatgacgccc
    10681 tgcgccccgc ccgcaaagcg caccatcgcg tgggcctgat cttcattttc cacggtgacc
    10741 atttccgacg atccggcctt tgccggacgt gccggcacca caattttcag atcgccacag
    10801 acctgctcaa tctctcccac cagatactgg gccatattga cgatatgtgc cgccaggtca
    10861 cccagagccc ccagtccggc ggtctcttta aaacagtgcc agtgaatagg ggaaagcggg
    10921 tcggccatat agtcttcgtt gtgggttccg tagaagtgga tcacctcacc aatttcgccg
    10981 cacgcaataa tctctttcgc caactgtgcc gtcgggtttt tcatgtaatt gaaacccacc
    11041 agcgttttca cccctgcccg cttcgcagcg tcgaccatct cacgcgcgtc gtgggcattc
    11101 agtgccagcg gcttttccga atagacgtgc ttgccgtggc ggatcgcctc cagcgccatc
    11161 tctttgtgaa gatggttagg ggagcaaata tccaccacat caatggccgg gtcggccacg
    11221 agcacccgcc agtctccggt tgagcgattg aagccaaacg cctgcgcgcg ttctgccgcc
    11281 agcgccggat tcacctcagc caccatctcg cgcaccagtt tgccgcgcag attgaacacc
    11341 gtcggtgcct gggcataggc aatggcatgc gccttgccaa tatacccggt gccaatcaat
    11401 ccaatacgaa cctctttcat cgtgatcctc acagtgcgcc gcgacggctc ttggcatagg
    11461 tgtcgaatgc caccgccaga acgataatta atccggtgat aatttgctgg tagtaggccg
    11521 agacgttcat cagtaccagg ccattaatca gaatgcccat gatgatggag ccgatgatag
    11581 tgccgccgat acgcccatat ccccccatca acgacgtgcc gccaataacc accgaggcaa
    11641 taacacgcag ctcaaaggag atcccggcaa ccgcctcagc gctccccaga cgagcgctca
    11701 ggatgaagcc cgccagcccc gccagacagc caatcagcac atagacgcta accagcacac
    11761 gcttcacgtt taccccagcc agacgtgccg cctccgggtt gccaccgatt gcatagacga
    11821 agcgccccca acgggtttta tgcagtgcca gataaccacc aatggcaacg atggcgaaga
    11881 tccagatcgg aatggagatg ccgagcaatt cccctcgtcc ccaccagcga taaccctgat
    11941 cgaagccggc aatcggcgca ccgtcgttaa tcacgagcgt cagaccacgc cagatggtca
    12001 tcccgccaag ggtgacgatg aacggtggta ggcgcaggcg ggtgacgccc aaaccgtgca
    12061 ggaagccaat cgctgtaccc atggtcagac aaatccccag cccaatcagc cagctcatac
    12121 cgccccaagc gttcggatcg actgtggtga aattgtcgcc tttgatcacg tacgccgctg
    12181 tcatggcgca cactgccaga atagagccca cggagaggtc gatcccagcg gtcagaatga
    12241 cgaaggtcat ccccacggcc atgattccgt agatggagac ttcggtcagg atgttgaaaa
    12301 tattgcgttc agagaaaaaa ttactgttct gcgactgaaa gaagatcagc agcaggatca
    12361 tgaaaatcag taccccgaag cgctcgaaga aggcaatggg atcgatgcgt ccggcgcgtt
    12421 ttaccgggac ctgcgtttta ggaatcatct gcgtcatgag aaactccgtt atgccgcgtt
    12481 cagggcgttg tggttgatag ccatcatcgt catcagccgt tcttcgttag cgtcgtcacc
    12541 gtggatctcg ccagttaacc ggccttcact aagggtgatg atccggtcag acaccgccat
    12601 cacttcaggc agatcggagg agatcacgat caccgccacg ccctttttcg ccatatcaaa
    12661 cagcacctgg tgcacttccg atttggttcc aacgtcgatg ccacgcgtcg gctcatcgac
    12721 aatcaacacc cgtggattga gcgccatgca gcgcgcgagg ataactttct gctggttgcc
    12781 gccagagagc ttacgcacct cttgcgtgct gttgaccatt ttgatctgca gcgcctggcg
    12841 gtaggactca atcaggtcgt cctctttgcg ggtattcaca aaccagcgcc actgcaacag
    12901 cgaggagagg caggagagcg acaggttgta gcgaatcgac agacccagca ccgccccctc
    12961 ttttttgcga tcttccggca ccagcgccag cccttgctcc agcgcataaa gtgggtcacg
    13021 cggctggtac ggcacgccgt ccagttcaaa gctgccggtg gtaaaggcgt cggcgccaaa
    13081 caggcagcgc gctacctcag tacgaccagc ccccaccagc ccagcgatac ccagaacttc
    13141 cccagcatgg acgtgaaaac tgatgtcatg cagggcgatg ccgtgtggat ccagcggcgg
    13201 tttttcccgg cttaacccct tcaccgacaa acggacaggt ttgtcttcat ggtgggtttc
    13261 gctggcaggg cggcggttaa aggccacatc acgccctacc atcaggcgga tcaactgctc
    13321 tacgttcgtc tccgtcactg cgccggtgcc ggtgaaacgg ccatcctgga aaacggtgaa
    13381 acgatcgcaa agctggaaaa cttcatgcag acggtgagta acgtaaataa tgctcacgcc
    13441 gcggttttta agctcccgca ccacgcggtg caggctttcc acctcgctgt cactcagggc
    13501 ggcggagggt tcatccatga tgatcagcct ggcattgagg gtcagcgccc gggcaatctc
    13561 caccatctgc tgctgggcca cgctcagacg ggcgactggc gtggtgggtg cgacattcag
    13621 ttgcagataa tcgagaatca cctgcgcttc ctgattgacc gctttttcgt cgacgatcag
    13681 gttacgttta cgcggttcgc gtccgagaaa catgttttcc gcaacggtca tattgggcag
    13741 caggttgaat tcctgataga tggtgataat gcccttgttc tgccgttcta ccggggagtc
    13801 gtccaccggc agcgtttcgc cattgaacca gatctccccg cgcgtttgtg gctgtgcgcc
    13861 cgcgagtgcc ttcaacagcg tggatttccc agcgccgttt tcgcccagca aagcgtgaat
    13921 ttcacccgtg ccgacggtga gctgggcgct gctcagcgcc catacgccgg aaaagctttt
    13981 tgccaggtcg gttactttca gtaagggttg cgacatcagc ctgctcctta tttatgggga
    14041 gccgcccgta cggcggctcc agacaaatca gttaccggcc tcagcaatac gctcagcctg
    14101 ttgcaggttc tctttggtga tcagcgtcgg cggatagtcc gccccggtga tagttttttt
    14161 ctcacgcacg ttagccacca gttggctcat cgccgtggtc accgcaaagc cgggacgctg
    14221 atccgccgta accgccagcc agccgtcgcg cacgcgggcc agtgcttccg gtaccgcatc
    14281 aaaaccggtg accaggattt cacccggttt cacaccctga ctctgcagcg cttcaatcgc
    14341 ccccagcgcc atatcatcgt tggcagagag gatcacctga ggacgttttg gcagcgacgg
    14401 caaaacgctt tcgacaatgc gcatcccttc cgagcgcatc cagttgccgg tctgatcggc
    14461 gacgatcttg tacatatccc cgccagcctt caggctgtca cggatccctt tggtgcgttc
    14521 aatgttggag gaggaacccg gttgaccggt caacagaatg atctccgcgc cgttggggaa
    14581 tttggttttg acgaagtcgc caatcgcctg gccgcccttg tagttgttgg caccaaagtg
    14641 cggcactttt ttctggctgt cgacagagcg gtcaagcgta acgaccggca gcttcgtatc
    14701 ctgaatttca tctaccgcac tggagaccgc gttcacatcg ttcggggaga cgataaagcc
    14761 ctgcgcacca cgggtgatgg cgttttccag gtcagccacc tgcttcggtg agctgccctg
    14821 accgtccagt acctgaagtt tcacccccat ctcttttgcg gctttcaccg ccgtgcgctg
    14881 catgtgaact tcaaacggca tggccaggtt tggcgtactg aaaacaattt gctcgttttc
    14941 ggctatggcg gccccggatg tcatggcgat tagggcggct aagatcagtt ttttcattat
    15001 tatgtctctc tgtgtagggt tgttgtgttt agaggacgat ctcacgctgg caacgcagtg
    15061 attccagcgc tttatccgct aggtacagcg cacgttcacc atcatcgccg ctgcaatcag
    15121 gcaccgcttc gccgcgcaga accgcaacaa agtgctccca ttccgctgcg taagcggatt
    15181 tgtagcgttg caggaagaag tgttccggca gcgcgctggt gcaaccctgt ttgccgtagt
    15241 gctgcacctg gttttcaagg atattgcccg cgcacagcag cccttcggaa ccgtgtagct
    15301 ccaggcgctg gtcgtagccg tatgaagagc ggcgactgtt gacgatggtc gccatcgcgc
    15361 cggaggcata tttcagaacg ataaatgcgg tgtcgatatc tcccgctccg ccaatcgccg
    15421 gctccaccag attgctgccc tgtgcataca ccgacaccgg ctcttcaccc atgatgaagc
    15481 gtgccatgtc aaagtcgtgg atggtcatat cacggaacat gccgccggaa acacgcacgt
    15541 actccgcagg cggtggagac ggatcgcggg agatgatcag cagtgattcc ggtttgccga
    15601 tacgcccggc ctgagtgtcg gttttcacgc gacggaactg tgggtcaaag cggcggttaa
    15661 aaccgataaa cagcggtacg tcatactctt tgaccacttt caggcagtcg cgtacgcggg
    15721 caagatcaag gtgtaccggt ttttcacaga aaatggcttt gccgtaacgg gcggccattt
    15781 caatcagatc cgcgtgggtg tcggtcgcgg aggcgatcaa tacggcatga acgttcggat
    15841 cggtcatcgc ctcatccagg ctctgctggc gcgcctgata ctgtgtcgcg agacgcgtgg
    15901 cgaactcctg attcgggtct accacagacc agagcgtggt cgcactatgg ctggcgatgt
    15961 tagctgcatg tacctggcca attcggccag cgcccagtaa agcaatgtta aacataacgg
    16021 ctcccggtgt tgatgaatgc ggtgaactaa cgacactaca aataaaacat ttat
//
LOCUS       DBBGLQ010000099        15828 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.099, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000099 DBBGLQ010000000
VERSION     DBBGLQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.099"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            67..342
                     /gene="yddL"
                     /locus_tag="ACIYSB_004121"
                     /pseudo
     CDS             67..342
                     /gene="yddL"
                     /locus_tag="ACIYSB_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            381..1711
                     /locus_tag="ACIYSB_004122"
                     /pseudo
     CDS             381..1711
                     /locus_tag="ACIYSB_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            2035..3423
                     /gene="narU"
                     /locus_tag="ACIYSB_004123"
     CDS             2035..3423
                     /gene="narU"
                     /locus_tag="ACIYSB_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGC5431050.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     HKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            3505..7245
                     /gene="narZ"
                     /locus_tag="ACIYSB_004124"
     CDS             3505..7245
                     /gene="narZ"
                     /locus_tag="ACIYSB_004124"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGC5431051.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            7242..8786
                     /gene="narH"
                     /locus_tag="ACIYSB_004125"
     CDS             7242..8786
                     /gene="narH"
                     /locus_tag="ACIYSB_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGC5431052.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGNKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGILQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            8786..9481
                     /gene="narW"
                     /locus_tag="ACIYSB_004126"
     CDS             8786..9481
                     /gene="narW"
                     /locus_tag="ACIYSB_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGC5431053.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            9478..10158
                     /gene="narI"
                     /locus_tag="ACIYSB_004127"
     CDS             9478..10158
                     /gene="narI"
                     /locus_tag="ACIYSB_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGC5431054.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            10237..11130
                     /gene="yddE"
                     /locus_tag="ACIYSB_004128"
     CDS             10237..11130
                     /gene="yddE"
                     /locus_tag="ACIYSB_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGC5431055.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKKIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(11226..12071)
                     /gene="nhoA"
                     /locus_tag="ACIYSB_004129"
     CDS             complement(11226..12071)
                     /gene="nhoA"
                     /locus_tag="ACIYSB_004129"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HGC5431056.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDNQSPEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            12244..12813
                     /gene="yddH"
                     /locus_tag="ACIYSB_004130"
     CDS             12244..12813
                     /gene="yddH"
                     /locus_tag="ACIYSB_004130"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGC5431057.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(12810..13043)
                     /gene="pptA"
                     /locus_tag="ACIYSB_004131"
     CDS             complement(12810..13043)
                     /gene="pptA"
                     /locus_tag="ACIYSB_004131"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGC5431058.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSMNA"
     gene            complement(13143..14279)
                     /gene="ydcC"
                     /locus_tag="ACIYSB_004132"
     CDS             complement(13143..14279)
                     /gene="ydcC"
                     /locus_tag="ACIYSB_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc5 family transposase"
                     /protein_id="HGC5431059.1"
                     /translation="MELKKLMGHISIIPDYRQAWKMEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHPDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     AHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     LEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLTGSGLS"
     gene            complement(14365..15509)
                     /locus_tag="ACIYSB_004133"
                     /pseudo
     CDS             complement(14365..15509)
                     /locus_tag="ACIYSB_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(15691..>15828)
                     /gene="ydcD"
                     /locus_tag="ACIYSB_004134"
     CDS             complement(15691..>15828)
                     /gene="ydcD"
                     /locus_tag="ACIYSB_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HGC5431060.1"
                     /translation="KQRVYFSDKNKRASYKIINYGDYYDVDYYDNNLKNEVFDWIGKW
                     S"
BASE COUNT         4023 a         3917 c         3696 g         4192 t
ORIGIN      
        1 ataaatgacg ccgtttaaaa ttcgtttaga aaatacgttt aacgataatt aaaaggattc
       61 tgttaaatga aattaaaaat agttgcggtg gttgtaactg gtttgttagc tgcgaacgta
      121 gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta tggcaaggtt
      181 accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac ttatgcccgt
      241 ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg tcactgggaa
      301 tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaagaa tctctgattg
      361 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
      421 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
      481 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
      541 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
      601 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
      661 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acatctatca
      721 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
      781 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
      841 ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat aggctttcat
      901 cagttactta ttttagtgcg gcacataatc aactagagtt tgtgcaatta gaatcttgcg
      961 aatggctgca atacctgaat ctcagccata atcaattaac tgatattgtt gcaggtaata
     1021 aaaatgaact cttactgctg gatctatccc ataataaact aacaagttta cacaatgact
     1081 tatttcccaa cttgaatacg ttacttatta acaacaattt gctttctgaa attaaaatat
     1141 tctatagcaa cttctgcaat gttcagacat taaacgctgc taacaaccag ttgaaatata
     1201 taaatcttga tttcctgact tatcttccat ctatcaaaag tttaagactg gacaataata
     1261 aaataaccca cattgatact aataatacat ccgatattgg aactttattc cccataataa
     1321 aacagagcaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc ctactatgca
     1381 gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc tggcaattct
     1441 ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg aattgtttca
     1501 ctatcctatt aaaaaagcat ataccccata taatatacac acttacaaga cagaggaagt
     1561 tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa acaatactta
     1621 ctgtaataac gattattaca atcaggcaat aagagaggaa ccgatagacc ttctggacag
     1681 atcgttttcc tccagttcat ggccttttta gtgaataaat catcactaat tctataagtg
     1741 tctggtcttg ttgataatat agttaatatg taacccttta gattcacgat ctgaagggtt
     1801 gaaacatgac atacgttcaa agattaaccc cgtttgaagg ttagcttgtt agttacattt
     1861 agtaacacat atctggctac ttaatcccca ttctgccccc gcggttacgc tttcttatct
     1921 attctttcaa tcagcacttc acccaattca tccttaattc taataactgg ctattgcgcc
     1981 ttacctcgtg ttttgtcttt ttttcattac caatgtgtgc atgtgaggaa caatatggca
     2041 ctgcaaaatg agaaaaatag tcgttatctt ttgcgcgact ggaaaccaga aaatccggcc
     2101 ttctgggaaa ataaaggaaa acatattgct cgaagaaatc tctggatatc agtcagttgt
     2161 ctacttcttg ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa tctcaataaa
     2221 atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct
     2281 ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg acgccgatgg
     2341 acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa
     2401 aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt
     2461 gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg
     2521 agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt
     2581 gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag
     2641 gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactggcg
     2701 attgccacga tcgccgcatg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt
     2761 gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt
     2821 gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag
     2881 ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg tgccatcgcg
     2941 cggtcggttg gtggtgctat ttccgataag ttcggcggcg tgcgggtgac gttgatcaac
     3001 tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg cacaggctcc
     3061 ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgactgc gggtctggga
     3121 agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcgggta
     3181 aagatgaaag gcggtagtga tgagcaagct cataaagaag ccgtcaccga aacggcggcg
     3241 gctctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcgttt
     3301 ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc
     3361 tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt cagccaaaaa
     3421 taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt
     3481 cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa
     3541 caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg
     3601 gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt
     3661 aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa
     3721 atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga acctcgcggc
     3781 tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct gaaatacccg
     3841 ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaagcaaca cagcgatccg
     3901 gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta caaacaagtg
     3961 cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct gattgccgcc
     4021 gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt ctcgccgatc
     4081 ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct gcttggcggc
     4141 acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg
     4201 ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta catcatcgcc
     4261 tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac cgaagtacgc
     4321 tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc caaattgtgc
     4381 gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc aatgggccat
     4441 gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc
     4501 cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg tagctacgtt
     4561 cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag caacaatccg
     4621 cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa cggttcgatt
     4681 ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtacggaa
     4741 accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt ggccttcccc
     4801 tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg
     4861 cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc ggtggtcagc
     4921 gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagt
     4981 gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc
     5041 gtgccgcgcc agtatattga aaccatcgcc cgtgaatttg ccgataccgc ccataaaacg
     5101 catgggcgct cgatgattat cctcggcgca ggtgttaacc actggtatca catggacatg
     5161 aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca aagcggtggc
     5221 ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg gttgccactg
     5281 gcctttgcgc tcgactggaa ccgaccaccg cgccaaatga acagcacctc gtttttctac
     5341 aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact ttcaccgctc
     5401 gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc cgaacgtatg
     5461 ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa agctgaagcc
     5521 gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta
     5581 cgtatggcct gcgaacaacc agatagcagc agcaatcatc cgcgtaattt gtttgtctgg
     5641 cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg
     5701 gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa
     5761 gaagtcgagt ggcaaactgc agcgattgaa ggcaagctcg acctgctggt gacgctcgac
     5821 ttccgcatgt ccagtacctg cctgttctcc gatatcgttc tgcccaccgc cacctggtac
     5881 gaaaaagacg atatgaacac ctcggatatg catccgttta ttcatccgct ttctgcggcg
     5941 gtcgatccgg cgtgggaatc acgcagcgac tgggaaatct acaaaggtat cgccaaagca
     6001 ttttcgcaag tgtgcgtggg tcatcttggc aaagaaaccg acgtggtatt acaaccccta
     6061 ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc
     6121 gaatgcgatc tgattccagg caaaaccgcg ccgaatattg tggcggtgga gcgcgactac
     6181 cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact tggcaatggc
     6241 ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa actcaattac
     6301 accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat tgacgcttca
     6361 gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa agcgtggcag
     6421 gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa agaggacgag
     6481 aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacctgg
     6541 tccggtctgg aaagcgatca cgtctcctat aacgcgggat acaccaacgt tcatgagtta
     6601 attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt
     6661 gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag
     6721 atgcgccaga taccgccaaa cggcttcccg gaaaaagcac ttaacttcct gacgccgcac
     6781 cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc
     6841 ggtggaccga ttgtctggat cagcgaaaca gatgcccgag aactgaccat tgtcgataac
     6901 gactgggtgg aagtattcaa cgccaacggc gcgctgactg cccgcgcggt ggtcagccaa
     6961 cgtgtaccgc cgggcatgac catgatgtat cacgcccagg aacgcattat gaatattcct
     7021 ggttcggaag taactggcat gcgcggcggc attcataact cggttacccg cgtttgcccg
     7081 aaaccaacgc atatgattgg cggttacgcg cagctggcct ggggctttaa ctactacggc
     7141 accgtcggat cgaaccgcga tgagttcatc atgatccgca agatgaagaa cgttaactgg
     7201 ctggatgatg aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaatc cgttcacaag
     7261 tcggcatggt gcttaacctc gacaaatgta tcggctgcca tacctgttcg gtgacctgta
     7321 aaaacgtctg gaccgggcgc gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca
     7381 aaccgggcat tggttatccg aaaaactggg aagatcagga agagtggcaa ggcggctggg
     7441 tgcgtgatgt gaatggcaag atacgcccgc gtctgggcaa caagatgggc gtaataacca
     7501 aaatcttcgc caacccggtg gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg
     7561 actacgaaca tttgcatagc gcaccggaag gcaaacatat tcctactgct cgcccgcgtt
     7621 cactgattga cggcaaacgg atggacaaag tgatctgggg gccaaactgg gaagaactgc
     7681 tgggcggtga gttcgaaaaa cgtgcccgcg accgcaactt cgaggccatg caaaaggaga
     7741 tgtacgggca gtttgaaaac accttcatga tgtacctgcc gcgcctgtgc gaacactgcc
     7801 tcaatcccag ctgcgtggcg acctgcccaa gcggcgctat ctacaaacgc gaagaagacg
     7861 gcattgtgct gattgatcag gataaatgcc gtggctggcg tttgtgcata agcggttgtc
     7921 cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc agaaaaatgc atcttctgtt
     7981 acccacgaat tgagtccggt caaccgaccg tgtgctcaga aacctgcgtg ggtcgcatcc
     8041 ggtatctggg cgtgctgctt tacgacgccg accgcatcga ggaagcggcg agcaccgagc
     8101 gcgaagttga cctctatgaa cgccagtgcg aagtgttcct cgatccacac gatccctcag
     8161 tgatcgagga agccctgaaa caaggtattc tacaaaatgt gattgacgct gcccagcgtt
     8221 cgccagtcta caaaatggcg atggactgga aactggcact accgttgcac cctgaatacc
     8281 gcaccctgcc gatggtctgg tacgttcctc cgctgtcacc gattcagtcc tacgcagatg
     8341 cgggcggttt gccgaaaagc gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg
     8401 tgcaatatct cgccaatatg ttgagtgccg gcgataccgg tccggtactg cgggcgctga
     8461 aacggatgat ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc gttactgata
     8521 ctcgtgccat cgacgaagta ggcctgagcg tcgcccaggt cgaagagatg tatcgctatc
     8581 tcgccattgc caactatgaa gatcgttttg ttatcccgac cagccatcgg gaaatggcgg
     8641 gcgatgcctt cgcagaacgc aacggctgcg gttttacctt tggcgacggt tgccacggtt
     8701 cggacagtaa attcaacctg ttcaacagta gccgtatcga tgccatcaac atcaccgaag
     8761 tgcgcgacaa agcggagggc gaataatgca gatcctcaaa gtgatcggcc tgttgatgga
     8821 gtatccggac gagctgttgt gggaatgcaa ggaggacgcg ctggcgttga tccgccgcga
     8881 cgcgccgatg cttacggatt tcacccacaa cctgcttaac gcgccgctgc tggataaaca
     8941 ggccgaatgg tgcgaagtgt ttgaccgcgg gcgcaccacg tcgctgctgc tgttcgaaca
     9001 tgttcatgcc gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg cggagtatga
     9061 aaaggtcggc ctgcagctgg attgtcggga actgcccgat tatctaccgc tgtatctgga
     9121 gtatttaagc gtactgcctg acgatcaggc gaaagaagga ttgctcaatg ttgcgccgat
     9181 cctcgccctg cttggcggtc gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga
     9241 tgctctgctg caactggcgg gaagctccct ttcaagtgac agtgtcacaa aacaagtaaa
     9301 cagcgaagag cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag aggaacaggt
     9361 gaagtttatt gaagataacg ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg
     9421 acgctttagc caggatgtcg cgccgcaata tgtcgacatc agtgcgggag gtgggaaatg
     9481 attcagtatc tgaacgtctt tttttacgat atctacccgt acatttgtgc gacggtgttt
     9541 ttcctcggca gctggctgcg ctacgactac gggcagtaca cctggcgcgc ctcctcaagt
     9601 caaatgctcg ataaacgcgg gatggtgata tggtcgaatc tgttccatat cggcattttg
     9661 gggattttct tcgggcactt gttcggcatg ttaacgccgc actggatgta cgcgtggttt
     9721 ttgccagtgg cagcgaaaca actcatggcg atggtgctcg gcggtatttg cggtgtgttg
     9781 acgttgattg gcggcgcagg actgctgtgg cgcaggctga caaatcagcg cgtgcgtgcc
     9841 acttccacta cgccggatat catcatcatg agcattttgc tgatccagtg tctgttgggg
     9901 ctaagtacca taccgttttc agcacagtat cctgatggta gcgaaatgat gaagctggtg
     9961 ggttgggcgc aaagcattgt cactttccgt ggtggatcgt cagagatgct caacggcgta
    10021 gcgttcgtct tccgcctgca tctggtgttg ggaatgacca tcttcctgct cttcccgttc
    10081 acccgactgg tgcatgtgtg gagcgcgccg tttgagtact ttactcgtcg atatcaaatt
    10141 gtgcgttcgc ggcgataatt tcaccctggc cggatagccc ttcgctctcc ggccacttgc
    10201 aggctatgat agttctccag tcacaggaga acgttaatga aaccgcaggt gtaccacgtc
    10261 gatgctttta cctcacaacc gtttcgcggc aattctgccg gtgttgtttt ccccgccgat
    10321 aatcttagcg aagcgcaaat gcagcttatc gcccgcgagt taggccattc ggaaactgct
    10381 tttctgctgc acagcgacga cagcgatgtg cgcatccgct actttacgcc aacggttgaa
    10441 gtgccgattt gcggtcacgc gacagtagct gcgcactatg tacgtgcgaa ggtgttaggt
    10501 ttaggaaatt gcacgatctg gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa
    10561 aagcacaacg atgattatcg tatttcgctg gaacaaggta cgccgggctt tgagccaccg
    10621 ctggaaggtg aaacacgtgc ggcgattatc aacgcgcttc atctcactga ggacgatatt
    10681 ctgccaggct tgccaattca ggtggcaaca acaggacact caaaagtgat gatcccactg
    10741 aaaccggaag tggatatcga cgccctttcg cctgacctta atgcgctgac cgctatcagc
    10801 aaaaagattg gctgcaatgg tttcttcccg ttccagatcc gcccaggcaa aaacgaaacc
    10861 gatggtcgca tgttctcgcc tgcgattggt attgtggaag atccggtgac tggcaatgcc
    10921 aatggcccga tgggtgcatg gttggtacat cacaacgtat tgccccacga tggcaacgtg
    10981 ttgcgtgtta aaggccatca ggggcgcgca ttggggcgtg acggtatgat tgaagtgacg
    11041 gtgacaattc gtgataacca accggaaaaa gtcaccattt caggcacagc ggtgattttg
    11101 tttcatgccg aatgggcaat tgaactctga aaccgttgtc tgatgcgctt cgcctgtcag
    11161 gtctacaacc gggctaatgt atgccggata aggcgtttac gccgcatccg gcaacctgac
    11221 ataaattatt ttcccgcctc cgggtgcgta tcaaacgccg ccatcaccag cgctaactca
    11281 tccacggtaa agccatgttt cgcatcatcc acgcccagac caaactgttc ttgcatcaca
    11341 gcatataatg acgccacatc cggtagattt cgctgctcca ccgcgtgccc attttcataa
    11401 tgggtaaaat gaaaattggt cagtgtcagc ttgccgccgt ccggcaaatg gcggcacatc
    11461 agcaaatgat ggcgaaaatg cgactgcggc cagtgcgccg accagaaatt gcccatcaca
    11521 taatcgcttt gttgctgctc gcagagatca aaacggtaca tcgactgcca atgctgatga
    11581 tgattaaact gcaacaccca atcatcaccc tcctgcaaca accgatactc tccgtgtggc
    11641 gtggtctgca cgagatcgga aactaaacga atcggcgcgg ttagcgtctg cccaccgaaa
    11701 ccgacatcag caatccattt ttcctcttcc agttccacca acagcaaacg atgggtgcgc
    11761 ggcggtaatg ctggcggatt tgataacact acgcgcccta acaagctgcg aacgttaaac
    11821 cccagctcgc gtaacacccg ctcaaacacg ccattctgct caaaacagta accgccacga
    11881 cgggctatca ccagtttctc ttccggcgat tgattatcaa gctgtatttc cctcggcagc
    11941 aaaacgtcga ggttttcaaa cggaatggtg caattgtgtt tcaggtgcaa tgcacgaagc
    12001 gtatcaatat tgaccgcagc agctcccgac cagttaatac gggcaaaata gtgattcaga
    12061 atgggcgtca tcaggcgttt cccttagtgg ttttctcaac ttataacctg gaatagacgc
    12121 cgcaccggct attttgtgcg atcgcggaca tgcatcacgt tcgcaaatcg ccaatcccgt
    12181 taatttcgtt acttattgcg ttacggacta cgcttaaaaa aaccttcttc gcgaggcgat
    12241 accatgagcc gattcatccc catagaatta caccatgcca gccgtctgtt aaatcatggc
    12301 ccaacggtct tgatcaccag tttcgatgag caatcccagc ggcgtaacat tatggctgca
    12361 gcctggtcaa tgccggtgga gtttgaacct ccacgcgtgg cgattgtggt agataaatcg
    12421 acatggacca gggagttgat tgaacataac ggtaaatttg gcatcgttat cccgggcgtt
    12481 gcagcaacta actggacgtg ggcggtggga agtgtgtcgg ggcgtgatga agataaattt
    12541 aattgctatg gcattccggt tgtgagaggc ccggtatttg gtttgcctct ggtcgaggaa
    12601 aaatgtctgg cgtggatgga gtgtcgattg ctacctgcga cttctgcgca agaagaatac
    12661 gacacgctgt ttggcgaagt agtatcagca gcggcagacg cacgggtatt tgtcgaaggc
    12721 cgctggcagt ttgatgatga taagctcaat acgttgcatc atttaggtgc tgggacgttt
    12781 gttaccagcg gcaagcgtgt tacggcgggt taagcattca tgctataacc aggtttcttt
    12841 atcaaagcct ccatttgggg cgcgatttcg gcatcccaga tagcttgcca agattctggt
    12901 tgaatctgct gtagagcaat gcttatcgaa ctgtctttac tgttcagatg acgaataata
    12961 acgtcggtaa tatctgcagc aagtgctgct ttttgttgtt cgtccagttc acgcggaaaa
    13021 catttaatgt cgatgtgcgg catgattacg ctccttttga aagatgtcag taaaattaac
    13081 acacattgtg aatgtcatgt gtccggtaaa gcgtttatac gacaataatg cccagggcaa
    13141 gattacgaaa gcccgctccc cgtaaggact gacgccaggt agtttctgtc catggctgct
    13201 tttcgcatct tacgtcttaa ccctgccttg aataccttat cattcgtcaa aatattaata
    13261 gcaatgtgcc gtatccctga aaataattct gctgcatttc ctcttcttat tttgcagtcg
    13321 tcttcattca ttaccacgtc cagacgccag tgcagcttat tctccacatg ccagtggttt
    13381 cggattgctg tggcgaactt ctctgcggtt aaatcagcag aactgatata atatctgacc
    13441 gtcatttcga gctctttctt ttgttctgct attatggacc gaaaggagac tgccacgcat
    13501 aatttcttca gccctttcca ttcaaacgtg aaatcaataa gttcatcagg gacatcgcaa
    13561 acaatatgaa gacggatttc ttctctgccg tgactctttt cactaattgc gtaactgtca
    13621 tgcgctggat tatttaattc tttcagcggg aatttttcct caaaggcttt atttagccgc
    13681 ccctggtttc cttttaccgc gaataaataa tcacctccct gtttttgtat cttctctgca
    13741 atatctttct ggcaacccat cgcatcagtt gtgatgattt ttcctttaat atccagcatg
    13801 ttaagaagtt ctgggatagc tgtaatctca ttagatttct catccgtctt gatctgtccg
    13861 atgaccagac tgtgcattgt tgagaacgca ctaatgacat gaatcgctcc cctgcggcga
    13921 ctcttatcat aagaatgccg gagcgttttt ccatcaattg caatgacgtc tttatcatct
    13981 gaagaatggc agtcacgcat ccagttaata aagcactcgt gaaattttgc aggactgata
    14041 caggatacaa ctctggcaat ggtgtcgtga acaggaatac cattttcaaa atcaccatat
    14101 tgcttcaaaa aatcgggatg tgtttcccca aaatcctcta tatcttccca gccttctgca
    14161 ccagaaataa cggcacaaat agtcaacagt agaatatccg ataacttatg ttccattttc
    14221 caggcttgtc tgtaatcggg gataatagaa atatgtccca tcaatttttt aagttccatt
    14281 ttgttctcct taattatgta aggagtattt gatcatgtgt aagcaataaa aaacagcttt
    14341 aggtaataag gaatatctca atttttaaac ataaaatgcg aattgtttag tacaaaaagt
    14401 gaacttctga tgatctttcc ctgtgtttaa cgcagccttc tttcttttcc tcttcaccct
    14461 taatgtcttt gcaatctctt aataaattca gtgccatctt ctttattcca gatattattt
    14521 aggctgcgtt tcctcttctt atccggctgg catcttcatt catttttaca tctaacaccc
    14581 aatcaagact gtgctcgatc agccagtgcg ctctgatagc atgtgcaaat tctttagcat
    14641 ccatatcctt tgatgaaata taatatcgga tgcttacacc ttctgctgat ttatcttctt
    14701 tcttctgcct gaatgacaat gctacacaaa gcttttttaa tcccttccat tcgaattcaa
    14761 aatcacaaaa gttcaggcgt tacgttactg acaatatgca aacgtgtttc ttttcttcca
    14821 tgacttatct cctgcgtact aaacgaatcg cctttataat tagaaaacac atttacagga
    14881 aatttttcct cgaatgcatg atgtaatttc ccctgattgc cttttactgc cagaagataa
    14941 tctgcttttt tatctttgat cttcgaagcg atatctttct gacagcccat agcatcaatg
    15001 gttatcaaat ttttctttaa atacagtagg ttaagcaact ctggaatggc tgtaatctca
    15061 ttacttttgg cttccgtttt cacctgcccc agtacaacac cattttcgtt cgagaatgca
    15121 ctcaccatat ggattgctcc ttttcttttt cccttatcaa aggagcctct tatggtcttt
    15181 ccatctattg ctataatttc accatcagtg atttcatggc actcctgcat ccattcaata
    15241 aacatctttt caaaggccaa actgtcaatg ttactcacaa cgcgtgcaat ggtgtcatcg
    15301 accggaatgc cattatcaaa atcaccatat ttctttagcc attcaagtct ttcatgtcca
    15361 aaatcttcaa tttcctgcca ctcatcggca cctgcaatta ctgcacatac ggtgagaaac
    15421 aaaatagcag ataatttatg tttaaccttt ccttgttgtc gtatatcagg ggtcactgaa
    15481 atataatcaa gcaaactttg aatactcacc tggcgtcctt ttttatcttt ttagttattc
    15541 caaaggttaa attttgatca cctgtaggcg ttaaagcaaa gatgaaaatc gtgatttaca
    15601 tcacaatttt catgatcttg ccctgccccg tcccttgctc tcatgtcttt ctgtatgtat
    15661 tttatatttt tctctttctg gtgttgttca ttatgaccat ttaccaatcc agtcaaaaac
    15721 ttcatttttc aaattgttat cataataatc aacatcataa tagtctccat aattaattat
    15781 tttataacta gccctcttgt ttttatcact aaaatacacc cgttgttt
//
LOCUS       DBBGLQ010000100        15508 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.100, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000100 DBBGLQ010000000
VERSION     DBBGLQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.100"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            29..595
                     /gene="yceJ"
                     /locus_tag="ACIYSB_004135"
     CDS             29..595
                     /gene="yceJ"
                     /locus_tag="ACIYSB_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGC5431061.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAARAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            599..1174
                     /gene="yceI"
                     /locus_tag="ACIYSB_004136"
     CDS             599..1174
                     /gene="yceI"
                     /locus_tag="ACIYSB_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGC5431062.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1216..2268)
                     /gene="trhO"
                     /locus_tag="ACIYSB_004137"
     CDS             complement(1216..2268)
                     /gene="trhO"
                     /locus_tag="ACIYSB_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGC5431063.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPESNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLCIPDPTE"
     gene            2493..3413
                     /gene="lpxL"
                     /locus_tag="ACIYSB_004138"
     CDS             2493..3413
                     /gene="lpxL"
                     /locus_tag="ACIYSB_004138"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGC5431064.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3585..4811
                     /gene="mdtG"
                     /locus_tag="ACIYSB_004139"
     CDS             3585..4811
                     /gene="mdtG"
                     /locus_tag="ACIYSB_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HGC5431065.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4894..5268
                     /gene="msyB"
                     /locus_tag="ACIYSB_004140"
     CDS             4894..5268
                     /gene="msyB"
                     /locus_tag="ACIYSB_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGC5431066.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5269..5496)
                     /gene="yceK"
                     /locus_tag="ACIYSB_004141"
     CDS             complement(5269..5496)
                     /gene="yceK"
                     /locus_tag="ACIYSB_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGC5431067.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5669..8212)
                     /gene="mdoH"
                     /locus_tag="ACIYSB_004142"
     CDS             complement(5669..8212)
                     /gene="mdoH"
                     /locus_tag="ACIYSB_004142"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGC5431068.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSFDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8205..9740)
                     /gene="mdoG"
                     /locus_tag="ACIYSB_004143"
     CDS             complement(8205..9740)
                     /gene="mdoG"
                     /locus_tag="ACIYSB_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGC5431069.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYETPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10134..11291
                     /gene="mdoC"
                     /locus_tag="ACIYSB_004144"
     CDS             10134..11291
                     /gene="mdoC"
                     /locus_tag="ACIYSB_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGC5431070.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAES
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11299..12780)
                     /gene="clsC"
                     /locus_tag="ACIYSB_004145"
     CDS             complement(11299..12780)
                     /gene="clsC"
                     /locus_tag="ACIYSB_004145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGC5431071.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12722..13255)
                     /gene="ymdB"
                     /locus_tag="ACIYSB_004146"
     CDS             complement(12722..13255)
                     /gene="ymdB"
                     /locus_tag="ACIYSB_004146"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGC5431072.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13350..13661)
                     /gene="ymdA"
                     /locus_tag="ACIYSB_004147"
     CDS             complement(13350..13661)
                     /gene="ymdA"
                     /locus_tag="ACIYSB_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGC5431073.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTVQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13782..14114)
                     /gene="csgC"
                     /locus_tag="ACIYSB_004148"
     CDS             complement(13782..14114)
                     /gene="csgC"
                     /locus_tag="ACIYSB_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGC5431074.1"
                     /translation="MNTLLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14173..14625)
                     /gene="csgA"
                     /locus_tag="ACIYSB_004149"
     CDS             complement(14173..14625)
                     /gene="csgA"
                     /locus_tag="ACIYSB_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGC5431075.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14666..15121)
                     /gene="csgB"
                     /locus_tag="ACIYSB_004150"
     CDS             complement(14666..15121)
                     /gene="csgB"
                     /locus_tag="ACIYSB_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGC5431076.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3862 a         3969 c         3693 g         3984 t
ORIGIN      
        1 ctctcgcaaa ttcgttttgg atgcacttat gtcattcaca aatacccctg aacgctatgg
       61 tgttatatct gcggccttcc attggctaag cgcaattatt gtctacggca tgtttgcttt
      121 aggtctgtgg atggtcacgc tcagttatta cgatggctgg tatcacaaag cacccgaact
      181 gcataaaagc atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt
      241 catatctcca ccgccgggtc cgctgccgag ttattcgccc atgactcgtc ttgccgcaag
      301 agctggacat ctcgcattgt acctattgct tttcgccatc ggcatcagcg gctatctgat
      361 ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct
      421 tgccgacgct ggcgcacagg cagactttgc cggtgccctg catttttggc tcgcgtggag
      481 cgtcgtagta ctgtccgtta tgcacggatt tatggccctg aagcatcatt tcatcgataa
      541 agacgacact ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat
      601 gaaaaaaagc ctgcttggtt taaccttcgc gtccctgatg ttctctgccg gttcagcggt
      661 tgccgccgat tacaaaattg acaaagaagg tcagcacgcc tttgttaatt tccgcatcca
      721 gcaccttggc tatagctggt tatacggcac ctttaaagat ttcgacggta cttttacctt
      781 tgacgaaaaa aatccggctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga
      841 tactaatcac gccgaacgcg ataaacatct tcgcagtgca gatttcctca ataccgcaaa
      901 atatccacag gcaacattca cctccaccag cgtgaagaaa gacggtgacg aactggatat
      961 taccggcgat ctgacgctga atggcgtaac caaacccgtc acgctggaag cgaaattaat
     1021 tggtcagggc gacgacccat ggggtggtaa acgtgcaggc ttcgaggccg aaggcaaaat
     1081 taagctcaag gacttcaata tcaagacaga tttaggtcca gcttctcagg aagtggatct
     1141 gattatttca gtggaaggcg tacagcagaa gtaagcgtga aatgttgccg gatggcacgc
     1201 atccggcaat gatatttatt ctgttggatc aggaatgcac agtgttgtat tcagacgtcc
     1261 acgagactta ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc
     1321 ttctggcggt aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc
     1381 cgcgcatact ggacactgaa taaacagcag gtggcagcca tcatttttac agttggtatg
     1441 gctgtcgcac ggcgcgccgc actggtggca atgcgcgata atctcatccg atatacgttc
     1501 gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca agccctgctc
     1561 gcgcgcctta cgggcgtatt caataattcc gccctcgata tgccagactt tgttgaatcc
     1621 gttatgtttc atccaggcac tggccttttc acaacgaatg ccgccggtgc agtacatgac
     1681 gattttttta tctttatgtg cctgcatcat ctcgactgct tttggcagct gctcacggaa
     1741 ggtatctgcc ggaatttcga gcgcgttttc aaagtgcccc acttcatact catagtggtt
     1801 acgcatgtcg ataaatagtg catcgggatc gtcaagcatg gcgttcactt ccgccgcttg
     1861 cagatactca ccaacattgc tggcatcaaa gtgaggatcg tcaataccgt cggcaacaat
     1921 gcgatcgcgt accttcatgc gcagtaccca gaaggatttc ccgtcatcat ccaacgcgat
     1981 attcaggcgt aaaccctcta aagccgggtc gaaggcatag agctgcgcgc gaaatgtttc
     2041 aacattgctc tcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca gatacactcg
     2101 cccaaaaaca ttcagcgcgg taaacagctg atataaagcg tcacgggtcg ccttaggatc
     2161 ggcgatgtgg aaatacttgt aaaacgaaat ggtggttcgc ggttcgctct cagccaacat
     2221 tttggctttt agcgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc
     2281 ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc
     2341 cacacatttt acgctacatt tgcgcattaa aaattatttg ttatttacaa gcgcggcaat
     2401 ttcgcccagt cttcagccac aaattttggt tgcgggcgaa aaaatgcgac aatacataca
     2461 attgcccgaa taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg
     2521 cactgcttca tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttagtcg
     2581 tgcaattgcc ctacccggtt atctaccgcc tcggttgtgg attaggaaaa ctggcgttac
     2641 gttttatgaa acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa
     2701 tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggcc
     2761 tgatggaaac cggcatggcg tggttctggc cggaccgccg aatcgcccgc tggacggaag
     2821 tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg
     2881 gcatccattt tctgacactg gagctgggtg cgcggcagtt tggtatgcag gaaccgggta
     2941 ttggcgttta tcgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt
     3001 tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga
     3061 aaaaaggcga agtggtctgg tacgcaccgg atcatgatta cggcccgcgc tcaagcgttt
     3121 tcgtcccgtt gtttgccgtt gagcaggctg cgaccacgac cggaacctgg atgctggcac
     3181 ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt
     3241 atcaattgat tatgctgccg ccagagtgtt ctccgccact ggatgatgcc gaaactaccg
     3301 ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt
     3361 ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatctccc
     3421 atgccggatg cttcagaatg gcatccggca ttaccacagc aaatccccct gatttagcga
     3481 taaaagctct ctggattgcg ccccctggaa gtcgggcgca taattagtgt gcttatcttt
     3541 tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa
     3601 atgacacccc tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta
     3661 ccggtgccgc cttcagtctg gtaatgccct tcttacccct ctacgttgag cagcttggcg
     3721 ttaccggtca ctccgccctg aatatgtggt ccggtattgt cttcagcatt acatttttat
     3781 tttcggccat cgcctcaccg ttttggggtg gactcgccga ccgtaaaggc cgaaaactca
     3841 tgctattacg ctctgccctc ggcatgggca tcgtgatggt gttgatgggg ctggcacaaa
     3901 atatctggca gtttttgatc ctgcgggcgc ttcttgggtt acttggcgga tttgtcccca
     3961 acgctaatgc tcttatcgcc acacaagtac cgcgtaataa aagcggctgg gcgctgggta
     4021 cgctctccac aggcggcgtt agtggtgcgt tgctcggccc aatggctggc ggcctgctcg
     4081 ccgatagcta cggcttacgt ccggtattct ttattaccgc cagtgtgctc atactctgct
     4141 ttttcgtcac cctgttttgc atcagagaaa aattccagcc ggtcagcaaa aaagagatgc
     4201 tgcacatgcg ggaagtggtg acatcactta aaaacccgaa actggtactc agcctgtttg
     4261 tcactacgtt aatcatccag gtggcgacgg gctcaattgc ccccattctg acgctgtatg
     4321 tccgcgaact ggcgggtaac gtcagtaacg tcgcctttat cagtggcatg atcgcctcgg
     4381 tgccaggcgt ggcggctctg ctaagtgcac cacgactcgg caaacttggc gatcgaatcg
     4441 gacccgaaaa gatcctgatt acagcgctga tcttttctgt actgctgttg atcccaatgt
     4501 cttacgttca gacgccattg caacttggga ttttacgttt tttgctcggt gccgccgatg
     4561 gtgcactact ccccgccgta cagacactgt tggtttacaa ctcgagcaac cagatcgccg
     4621 ggcgtatctt cagctataac caatcgtttc gtgatattgg caacgttacc ggaccattga
     4681 tgggagcagc gatttcagcg aactacggtt tcagagcggt atttctcgtc accgctggcg
     4741 tagtgttatt caacgcagtc tattcatgga acagtctacg tcgtcgtcga ataccccagg
     4801 tatcgaactg atttttcgcc tttcatactt gcaaaagcgg agaatcagct atccttttcc
     4861 ctgaaacctc atcaactcaa agggagaatc gtgatgacca tgtacgcaac gcttgaagaa
     4921 gccattgacg ctgcacgcga agaatttctt gcagacaacc ccggcatcga cgccgaagat
     4981 gcgaatgtgc aacagttcaa tgcccaaaaa tacgttttgc aggacggcga catcatgtgg
     5041 caagttgagt tttttgccga cgaaggggaa gaaggtgaat gtttacctat gcttagcggt
     5101 gaagccgcgc aaagtgtttt tgatggcgac tatgatgaga tagagatacg ccaggagtgg
     5161 caggaagaga atacattaca tgaatgggac gagggggaat ttcagcttga gccaccgctg
     5221 gataccgagg aaggacgcgc agcagctgat gagtgggatg aacgttaatc actcatacgg
     5281 gccatgatga atgtcgatcg gcagcagtaa agtatcaaaa accagagaga atggcagatc
     5341 aaggatcgtg acataacgcc aggcggagtc acgcacatcc cattgcacac cgggataata
     5401 ttggttgcca tgcccctgcc ccggtatagt gcgactaata atgctgccac agccactcag
     5461 caaggtcacc atgatgctca ccacaattaa acgcatttga tacctctttc gtaaaaaaat
     5521 accggcataa cgccggtatt tttattggct cgttgttttt gccggatgcg gcgtgaacgc
     5581 cttatccgac ccacaaagtc ttgcaaaatc aataaattgc aggaacgatg taggcctgat
     5641 aagcgtagcg catcaggcaa ctacgttttt attgcgaagc cgcatccggt ttacgcaatg
     5701 ccagtggatt gagctttatc ccttcgtaat aactcaccca tgaagaatat ctctccgggg
     5761 aattccagac acggaaatgc agacgggcca tcgtcaccgg atcgcttagc agcaccaggc
     5821 gacgatcgcg attcagcttc tctggcgtct cgttcagcgc ctgttcaacg tggcggtcac
     5881 gggcgatttc cagcacctta ctggcgcggt gacgcgcggt cgccattgcg gttgccagag
     5941 cgttaaatga cgggttaaac actgcgtgca taaagccatc atcaaaggag cgttgacgat
     6001 tcatctcaag gaaccgatcg gtatcaacca gcacctgcgg cggcgaatac tcttccggga
     6061 tcaggaacag tttccagcgt ttggtgcgca gaccaacggt ggcacggctg gaaatcaccg
     6121 aaacaaacgg tgacaggatc aacgagaaga caatcggtgc cagccagaac aggaaacgca
     6181 gatccagcca cgccatccca acagcccaca ctaaccctaa cagcagctgt gagccgtggc
     6241 gtttgaacgc ttcaccccag gaagtggagt catcatcacg ctgcggtgaa ttccacacca
     6301 cttcccagcc aaggaacgcg ctgacaacga agaccgtatg gaacagcatg cgtaccggag
     6361 ccagcagcac ggaaaaaagc acttccagca gcaacgataa tgtaacgcgc cagaagccgc
     6421 cgtattcttt cgttcctttg caccagataa gcaaaatgct caataacttc ggcaggaaca
     6481 acagcaccat ggtcgaagca aaaagtgcaa tcgccagctc aggacgccac tgcggccaca
     6541 ctgggaacaa ctgccgtggt tgcaggaagt attgcggttc ggtcaacgca tgcactacct
     6601 gcaatgcagt agagagcgcg aggaacataa accacagcgg agcggagaga taagacatca
     6661 cgcccgtcag gaacaccgca cggtgaaccg ggtgcatacc cttcaccagg aacagacgga
     6721 agttcatcag gttaccgtgg caccatcggc ggtcacgttt tagctcatca agcaagttag
     6781 gcggcaattc ttcataagaa cccgggagat cgtaagcaat ccagaccccc caacctgcac
     6841 ggcgcatcaa cgccgcttcc acgaagtcat gtgacaggat tgaaccggca aaggaacctt
     6901 cgcccggcag cggagccagt gcgcagtgct cgataaacgg tttcacgcgg ataatcgcgt
     6961 tatgtcccca gtagtgcgac tcgccaagtt gccagaagtg caaaccggct gtaaacagtg
     7021 gcccatacac gcgggtcgcg aactgctgac agcgcgcata cagcgtatcc ataccggacg
     7081 ctttcggcga cgactgaatg atcccggcgt tcgggttggc ttccatcagg cgcaccagcc
     7141 cgcacaaaca atcaccggtc attaccgagt cagcatccag caccaccatg tagctgtact
     7201 ggctgcccca gcgacggcag aagtcatcga tattaccgct tttacgcttc acgcggcgac
     7261 ggcggcggcg atagaaaatc tgaccttcgc caccgacttc agcgataagc tccatccagg
     7321 ctttttgctc tgcgacgcag atatccgggt tataactgtc actaagaatg tagacatcaa
     7381 agtgtttggc attcccggtg gcttttactg attcccacgt tgcacgcagg ccagcaaaaa
     7441 cacggttcac gtcttcgtta cagataggca tgatcaacgc cgtgcgatgc tccgggttta
     7501 atggttcatc gccaactgtt gacgcagata tactgtattt atcgcgacca ataagcagtt
     7561 gcaggaagcc cattaacgcc gtccagaatc cggcggacac ccaacagaac agtaccgcaa
     7621 agaggatcag gataccggtt tgcagcatat aaggcagaag ctgcataaag gaaacccaca
     7681 aatcctgacc aaccatatcc ataggattaa tcagcgccca accctgataa ggaagaatgg
     7741 tcttcatata ccaggtcgcg acgacagttt gcgcgagcgt caggatcaac agaatgtaac
     7801 ggcggatggt accgacggta cgccactttt gctcgctctc ctgctcttct ttggtcaaac
     7861 gagccagata gcgcggcgtg acatcgcgtc cacgcaggcg atcccagaaa cggcctaccg
     7921 ggttggtacg ccacgggtcg ggaaacatcg aggagcgttt tgcttctggc atcgccttca
     7981 gctgatcgcg cccttcgtcg tctttaatta actgtccatc agcaagtgaa tctggccagg
     8041 cttgttccag acgcgccttt accgagcctt gcggggaatc atcctcccgc gcccaggtgc
     8101 ggtgttcggc atccagcgcc tgatgaacgg cgcggatatc agtcttcggc aatgccgctt
     8161 tctcgcttgc ggcgatgggc attgcgtcaa tgtactcagt tgtcttattc attggcaggt
     8221 aactggtagc tccaggtttc actcaacgtc tgatcggcat tcaccagcgc agcacgcatt
     8281 tcagtggttt tcttggcatc tttcactttc acacgcatca ccagacgcca gcctttggta
     8341 accgggttat agcgcaccgt gctttcaact atctcaccat tatcaccaat gctggtttgc
     8401 gctgtgaccg gggtatcctc tggcagtttt ttcatctctg cgccggtaaa atcgaccaca
     8461 aaggcgatag taccgtcagg ctggcgaatc aggttcgact gcttcacatc ccccgttgaa
     8521 cgacgcgttt gttgcaccca tgcgttatct ggcgcatgca gtttgtcttc atcacggctg
     8581 aaggtgatgg tgtatttaaa gttcatctct ttacccggct ccggcagctg atccggcgtc
     8641 cagtaagcga cgatgttatc gttggtttca tcgttggttg gaatttccac cagctcaacg
     8701 ctgcctttac cccactcccc tttcggagtc acccatgcgc ttggacggag atcgtaacga
     8761 tcatcgagat cttcaaagcg ggagaaatca cgaccgcgct gcaacagacc aaagccttgc
     8821 gggttttcca tggagaagct gctgaccgct aaatgtttcg ggttattcaa cggacgccag
     8881 atccattcgc cattaccggc atggatagag agaccgttag agtcgtgcaa ctccggacga
     8941 tagttatttg caggcgacgg ttggttcggc ccaaacagga acatactggt taacggtgca
     9001 acccccagtt tgccgacttt atcgcgcaga tagattttcg actgcacatc cacaaccgtg
     9061 tcacgccctg gcataaccac gaatttgtaa gcacctgtcg cacgcggcga gtcaagcaat
     9121 gcatagatgg ttaaacgttt atcagtcggt tttggacgct cgatccagaa ctctttgaag
     9181 cgcggaaatt cttcacccga tggcaaggcg gtatcaattg ccaggccgcg ggcagaaagg
     9241 ccataaacct gacctgcacc aatcacgcgg aaatagctgg ccccgagcat gctgacgatt
     9301 tcatcgtttt tatctttgct gttgatcggg taaagcactt taaaaccggc aaagccaagg
     9361 tcttttaccg tatctttgtc atgctgaaca tcgccgaaag tgaaataatc cgggctgtat
     9421 ttgattcgtt tgactgcggt ggcagtcact tcatttattt tgaccggggt atcgaagtac
     9481 ataccctgat ggtagaactc gagtttgaat ggggtcttca gattgttcca gtacgctttg
     9541 tcatgattaa actggatctg ctgatagtcc gcgtatttca tatcgcggaa aacggagggc
     9601 aagttgcttt tgggcgtctc gtagcctttc ccggctaaag attgagcttg ctttgcgaca
     9661 tcatcaatac tgaaagccca gcttgaagat gtatacaggg ttaacattac tgcagcactc
     9721 aaccaacgca ttttcatcat ttgtagttta tgtttcataa ttagtaagca cttccccctt
     9781 tgtgtgctta tatcgatccg atctatttta atggataacc agccattccg acaacagaat
     9841 cccggtatcg ttcagcacgc tggctcatgg attaagcacc tgattgcaac cgttttcact
     9901 ttccgtgctt ctcctaaaga cagaagcatc gacttcgtgt agggtttgcc agcaaattta
     9961 tttatcaacc gtcttacaga cgcttccatt ctggttagtg gtagcgatga tgctatgcat
    10021 aaaaaccttg aagcctgcgc ctggcgcggt caggtcggtg tcactatggc aacaagccta
    10081 aaggcataac ccgacaggga taagacgaaa aatccgagat tagttaacca tatatgaacc
    10141 cagtacccgc gcaacgtgaa tatttcctcg actccatccg cgcctggctg atgttgttag
    10201 ggataccttt tcatatttct ttaatctatt cgagccatac atggcatgtg aatagcgccg
    10261 aatcatcatt gtggctgacc ctttttaatg acttcatcca ctcgttccgc atgcaggtat
    10321 ttttcgttat atccggctac ttttcctaca tgcttttttt acgctatccc ttgaaaaaat
    10381 ggtggaaagt acgtgtcgaa cgtgtaggta tcccgatgtt aacagccatc cccctactga
    10441 cattaccgca atttattatg ctgcaatatg tcaaaggaaa agcggaaagt tggcctgggc
    10501 tgtcattgta tgacaaatat aatacgttgg cctgggaatt aatatcacac ctgtggtttt
    10561 tactggtgtt agtggtcatg acgacgctgt gcgtatggat atttaagcgc atcagaaata
    10621 atttagaaaa ttctgataaa acgaataaaa aattctcgat ggtaaaacta tcggtgattt
    10681 ttttatgcct cggcatcggt tatgcggtaa taagaagaac gatttttatt gtgtatccgc
    10741 ccattctgag taatggcatg ttcaatttta ttgtcatgca aacgctgttt tatttgccgt
    10801 tctttatcct cggcgcactg gctttcattt tccctcatct taaagccttg tttaccacgc
    10861 cgtctcgtgg ctgtaccctt gcagcagcat tggcgtttgt cgcttattta ctcaaccagc
    10921 gctatggcag tggcgatgcc tggatgtacg aaaccgagtc ggtgatcacc atggtcctcg
    10981 gtctgtggat ggtgaatgtg gtcttctcct ttggccaccg tttgcttaac ttccagtcag
    11041 cgcgggtgac ttattttgtt aacgcatcgc tgtttatcta tctggttcac cacccgttaa
    11101 cgctgttttt cggcgcatac attacaccgc acatcacctc caactggctt ggttttctct
    11161 gtggcctgat atttgtagta gggattgcga taattctgta tgaaattcat ttgcgcatcc
    11221 cgttactgaa gtttttgttc tctggtaaac cggttgttaa gcgtgagaac gataaagcac
    11281 cagcccgtta agccacattt acaataacca ttccacgggc aatatcgacg ccagtctgac
    11341 cataacccgc ttccagaaac tggtggcggg ttcttttttg agaataatct ctttctttgc
    11401 atgacgatca acccagttga tccgtcccca cctgtccaga cggagctgcc aggccgcatc
    11461 atactggctc tgaataaagc gtttatcaat taactgtgcc agcgtttcgc tctctatcac
    11521 gaagcccatt tcagtattga gcaatgttga acgcggatcg aaattgaaag agccgataaa
    11581 caccgtttta ccatcgatgc taaaggtttt agcatgcagg ctggctccgg aattaccggt
    11641 tatgccgcga tcgtgtaacg tactactttg ttcacgcgtc ggcttgagtt catataattc
    11701 cacgccatag cggagcaatt ttttgcgcca gcgcgcgtat ccggcatgga cgacagcaac
    11761 atcgttagcg gcaagagaat tggttaggat cgcaatcttt accccttttc tcaccatccg
    11821 taagagttgc gccacacctg cgcgtgtcgg tacaaaatag gaagagataa tatcgatgcg
    11881 ttcactgggt gagcccatga tatcgaacag gcgctgcggt aacagtgaat gccgttttgc
    11941 cttgccctcc cctttcgccg gatcatcact taataaacgt gtcttcgccc agataagcgg
    12001 caatgttcca tcaaccagat gatttataaa tggactggat tccattttgc gtaaataacg
    12061 atgcgtcatg gcatcgttat gccaggaggc gggtaactcg atgcgatccg ccatttcacc
    12121 ctccgggaca tccagcacct gctgtaaggg tgaaaccgat ttgcaatacc agtagcgggc
    12181 gaaatcatcg gcaacgtcct ctaccacggg tcctattgcc atgacatcta aatccgaaaa
    12241 aagtggctcc tcccctgctc caaaataggc atcaccaata tttcgtcctc ccaccagggt
    12301 caccacgcca tcgacagtga aacttttatt gtgcatacgg cgattaagac gggaaaagtc
    12361 ggtgatataa ccaagcggac gcagcaagcg aaacgagaaa ggattaaaaa gccggacttc
    12421 aatgcgtgga tgactgtcaa gcaagcgtaa aatgtcgtca agtccgggcg tattgttgtc
    12481 gtccagcaac aaacggacgc gaacgccacg ctttgctgcg gctaacaggg cggaaaacag
    12541 taaccgaccc gacatatcgt cctgccagat gtaatactga acatcgagcg tatgctctgc
    12601 catttcggcc agacgatacc gggcggcaaa cgcatccaga cttttctcca gaggaaaaag
    12661 gccacactga ccgggatgtt gcgaacacag tggcagcacc gcgctcgcca gccggggcaa
    12721 atcattcatc tccttgttgg gtaaggagtc tttcgtagag gtgggcgttt tcttcatcat
    12781 aacagacaaa gtatacctgt tcgggtaaag cgtgacgggt aataaattct gaaacggttt
    12841 ttactgcgat ttcagccgct gccgcacgag ggtaaccata aaccccagta ctgattgcag
    12901 gaaaagccac tgacgtatag ctgtttgccg ccaccagtcg taggctattg agataggcat
    12961 cctgcaaaag ctggtcttcg ttttgttcac caccacgcca gactggcccg acggtgtgca
    13021 ctacggcttt agcgggaaga tcgcctgcaa gcgtaataac ggcatgcccc gtagggcaat
    13081 cgccctgctg ttgcctgact tttaaacaag catccagcag ggccggaccc gctgcgcgat
    13141 gaatggcccc atcgacgccg ccgcctccca ttaatgacgg attagccgca ttcacaatca
    13201 catcaacggc cagtttggta atatcaccct gcacaacatg aatacgcgtt ttcataacgc
    13261 ctccttacac cttcttgttt cttaagtgta cagcaggctt tttggttaaa tcgcattttt
    13321 ctgctcagca acgtgagttg ttcagaaact tagttatatt cgatgttcat tacagccagc
    13381 tttttctgtt tatcaagata ctgcactttt actgaggcta tttgcgcgtt tttgacattg
    13441 ccgctcatta acgctttgct ggagtaggtt ttgcccggaa tagaaccatt ctgtgggcag
    13501 ttcatttcta cgggtgatga ctgggtgctg acgtcacatg ccggttccac aatttggcca
    13561 taaaaatgga tcacgccccc ttgcacggtg cttccagcaa tggcaataaa aggaaaaaac
    13621 aaactgccga gcataagaga gtttaaaaaa ggtctgaaca tttattctat ttcctcaatg
    13681 attagtcatc cttgagggtt gtgttatcca tactttcagg ttggctgatt cgcagcagac
    13741 cattctctcc agattcatct tatgctcgat atttcaacaa attaagactt ttctgaagag
    13801 ggcggccatt gttgtgataa atgaagtgac tgtccatcag aaacagtaac aactattttc
    13861 acccgatcgt ccggggaaat atttaaactc aacttcgtca aagcaatggg ttgattagca
    13921 ggcaatgaaa gggtcttttc ttgcttcgtc tgactttgcc ctgaactgcc ttcgcgcagg
    13981 gacaatattt gtactctgca cagacaagat tgagtaagag tgacttcagg aataatggta
    14041 tacacatccc cttgctgggt cgtattaaag gttatctgac tggaaagtgc cgcaaggagt
    14101 aataacgtat tcatattctt ctcccgaaaa aaaacagggc ttgcgccctg tttctgtaat
    14161 acaaatgatg tattagtact gatgagcggt cgcgttgtta ccaaagccaa cctgagtcac
    14221 gttgacggag gagttagatg cagtctggtc aactgcagca ccgttgccac caccgaactg
    14281 tttaaccgtc atttcagaat ttttgccgtt ccactgatca agagtagcgc tgttaccgaa
    14341 gccacgttgg gtcagatcga ttgagctgtc atctgagccc tgaccaacat ctgcaccatt
    14401 accgccgcca tgctgggtaa tagtcaagtc agagttacgg gcatcagttt gcagagcaag
    14461 tgcagagtta ccgccaccgt actggtaaat gttcagctca gaatttgggc cgctattatt
    14521 accgccacca ccgtggttac cgccgccgta ctgaggaaca acacctgcca gagcgctacc
    14581 ggagaatacg attgctgcaa ttgctgctac ttttaaaagt ttcatgtaaa acccccatcg
    14641 gattgattta aaagtcgaat ggaaattaac gttgtgtcac gcgaatagcc atttgcgact
    14701 gtctctgcac tacaattgcc gttttttgag taccatactg tgtaatattt gctttattac
    14761 cagaaccttt ctggataatc atcgcagtat taccataagc accttgcgaa atactggcat
    14821 cgttggcact gcccgcctga tcaatatatg caaggttata atctcctgtc tggtcaatct
    14881 ttgcccggtt gctactacct tcttgcgcaa caaccgccaa aagttttgag cctccctgcc
    14941 gtaactgagc actattatta gtcccagctt gaccaattat ggctgcctga ttaaatgaag
    15001 acttactcaa ttcatttacc gcgaagttat attctgaatt agctaaatca taacctgctg
    15061 cggctgcaat cccaggcgca cccagtattg ttaacatcat aaataacaat ttgtttttca
    15121 tgttgtcacc ctggacctgg tcgtacattt aagaaattaa atcatttcaa cttggttgtt
    15181 aacgcaacct gtattttgtt aacgctgcgt tacgatggaa agtatgtctg cggaaatatt
    15241 tttaataact cacccgcgcg ttgtattttt cttttttagt tcataccaaa gtattaaaac
    15301 ccacgtaaat acgctgttat ctacgcaaaa aaatattttg ttttctttta aatctccgtt
    15361 ttccgctatc aaaaagcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
    15421 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaatt gtccacggag
    15481 gtgtggagaa aaaacaagaa cgttttac
//
LOCUS       DBBGLQ010000101        15456 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.101, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000101 DBBGLQ010000000
VERSION     DBBGLQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.101"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(12..971)
                     /gene="ybtA"
                     /locus_tag="ACIYSB_004151"
     CDS             complement(12..971)
                     /gene="ybtA"
                     /locus_tag="ACIYSB_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HGC5431077.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            1228..2940
                     /gene="ybtP"
                     /locus_tag="ACIYSB_004152"
     CDS             1228..2940
                     /gene="ybtP"
                     /locus_tag="ACIYSB_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HGC5431078.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVILVLARQRSEN
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFYYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            2927..4729
                     /gene="ybtQ"
                     /locus_tag="ACIYSB_004153"
     CDS             2927..4729
                     /gene="ybtQ"
                     /locus_tag="ACIYSB_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HGC5431079.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGYALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            4722..6002
                     /gene="ybtX"
                     /locus_tag="ACIYSB_004154"
     CDS             4722..6002
                     /gene="ybtX"
                     /locus_tag="ACIYSB_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HGC5431080.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIVAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     LVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            6030..7334
                     /gene="ybtS"
                     /locus_tag="ACIYSB_004155"
     CDS             6030..7334
                     /gene="ybtS"
                     /locus_tag="ACIYSB_004155"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HGC5431081.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRSWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(7528..8790)
                     /locus_tag="ACIYSB_004156"
     CDS             complement(7528..8790)
                     /locus_tag="ACIYSB_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5431082.1"
                     /translation="MFLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYQAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNLA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(8952..9027)
                     /locus_tag="ACIYSB_004157"
     tRNA            complement(8952..9027)
                     /locus_tag="ACIYSB_004157"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8992..8994),aa:Asn,seq:gtt)
     gene            complement(9128..9925)
                     /gene="mtfA"
                     /locus_tag="ACIYSB_004158"
     CDS             complement(9128..9925)
                     /gene="mtfA"
                     /locus_tag="ACIYSB_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGC5431083.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMSNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            10019..10108
                     /locus_tag="ACIYSB_004159"
     tRNA            10019..10108
                     /locus_tag="ACIYSB_004159"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10053..10055,aa:Ser,seq:cga)
     gene            complement(10136..11186)
                     /locus_tag="ACIYSB_004160"
                     /pseudo
     CDS             complement(10136..11186)
                     /locus_tag="ACIYSB_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(11186..11389)
                     /locus_tag="ACIYSB_004161"
     CDS             complement(11186..11389)
                     /locus_tag="ACIYSB_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGC5431084.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            complement(11448..13901)
                     /locus_tag="ACIYSB_004162"
     CDS             complement(11448..13901)
                     /locus_tag="ACIYSB_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGC5431085.1"
                     /translation="MSTDKETFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVHLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPP
                     PSAQDKPASTDNTNVNGEDMTEIEENMLLPVSGQILPVRWLAQHGGEKPVTHVSRDEL
                     RSLHNAQEEKLPAVTALAISNKAAQLEPLEIRDLYKLVRDTDKVFPAPVNSDLGLMTS
                     FIEAYLDADYTDRGLLTKEWMKGNRVSRISRTPSGANAGGGILTDRGEGFVHDNASMA
                     FDVATGKLARSMDVDIYNMHPSHTRRVNEIIAENDPCFSVFLKAFLNMPGGMDYSRAI
                     VVASVKEAPVGIEAMPQRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKPQPSGAMADEQPTTETVEPDTTEHHPDTQPLDAQPQVNSVDAKYQKLRAE
                     LHEARKNIPPKNPVDADKLLAASRGEFVEGISDPNDLKWVKGVQTRDSVNQNQQETEQ
                     NDQKAEQNSQNALQNEPETKQPEPVAQQEPEKVCTACGQTSGGNCPDCGSVMGDATYQ
                     ETFNGENQAESQEKNPEETEGTEHPHKENTGGAQGHASDNKTGETENSLIKMNGHREI
                     TSTSRVWNHLSVDLETMGANTNAPIVVIGAVFFDPETGEIGPVFYIVISLTDAMNTGA
                     VPDGGTIEWWLKQSSEARAAILTDQVKMKDALLRFRQFVNEHSDEKFVQVWGNGATFD
                     NAILRTSYERLDIPCPWHYSNDRDVRTIVEMGKITDFNARSVIPFEGVRHNALDDALH
                     QAKYVTATIQKLIPNQADF"
     gene            complement(14021..14242)
                     /gene="kil"
                     /locus_tag="ACIYSB_004163"
     CDS             complement(14021..14242)
                     /gene="kil"
                     /locus_tag="ACIYSB_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGC5431086.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVIYEGRTYIASANNIEKQKLYIR
                     DFTTKTCITDRMIKVFLGRDGLPVKAEAW"
     gene            complement(14288..15139)
                     /locus_tag="ACIYSB_004164"
     CDS             complement(14288..15139)
                     /locus_tag="ACIYSB_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGC5431087.1"
                     /translation="MLNVAIENQYGWNYSAPAPHKPGAGRGNPKSNVAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPGSTLTGISTPVRLTTIMVVESLGGELSILNVEAAIMAT
                     TLTLSHPDVTIENGRAVTTSVAIAEFFGKRHDNVIQKIKLLDCSAEFTALNFKASEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEEELRRNNTTPADKI
                     IPGDGRTLVIRFDEHGYIKFTETVPDGALVCTLDTFRFYLEKQGWTLINRGAIKNMTV
                     EQLLSIK"
BASE COUNT         3371 a         3860 c         4190 g         4035 t
ORIGIN      
        1 cgcgaccccg gttacatccc gcgtttaaag gtcgaaggag ttacgccaaa ctgtttctgg
       61 aaggcggcag agaaatggct ggcgttggca tagcccagat cggccgccac cgtcatgacg
      121 gaggtattgc cgttggccag gcgtcttctg gcttcctgca ttcgttcagc ctgaaacagg
      181 ccatagacgc tgttgttgaa cagaacgcgg aagcctcgct taattttgag aacgctcaga
      241 ccggactctc tggccagctc cgcgacgccc ggcgcttgcg tcagatcagc cagcagcaga
      301 tccttcgccc gcagcagttt ctggcgttcc atcgggttga agtggcagga gaggcaactg
      361 gcgtcttcgc gatgttcatc cagcaccaga ctgagcatga ccagactttg cccctgcaac
      421 catagcgtcg acggcttttg ttcatgactg aagccgccgt gcatgcgcat cagcgcgtga
      481 cgcaaggcct gagccgttaa gtgggtttca gcattacaac gctgctgctg gcaacagcag
      541 tgggagtcga tcttattctt aatgaccgcg ctaatatccg gcacccacag cgcgagggtt
      601 tcagggctga aggagagggt aatgctctcg aatcggccac aataggaggc gctgccgcga
      661 cagtctggcg tatgggtaat gtaatggcga ttgcgcgcaa gctcccagtc ggtgtggcgc
      721 cggagtccct gaatcgcaaa gcgcgatgtg ccttccagga tgcacgagaa gttaatcctg
      781 cccagatcgt cgtgtagctg caacgtctct ggccgcgcaa aatggcccct ccagagcaaa
      841 atatccatac cttcctgcaa cgaacagcgc agcagttcac actgagcagg aatattgcca
      901 tcgtttgcag gtttcccgac caccaactgg tgaatagaga tttcagattg cgtttgcggt
      961 gactccgtca tgacctggtt atctccctgt ggcttagacc aatgcgggta taaatagaac
     1021 cgaatggggt agatgagtta ttgataatgg ttattattca cattaattct cagaacgcca
     1081 ccctgaattg tcgtttttgg cgtccgtaaa aattcatcag gaaattcagt tactcccatg
     1141 tcatctcaat catccaatac tgaatcgctg tcccgatttc ctctctggca ggtgattaca
     1201 ccggttcgca ggaaggttat tttagctatg gcgctggcgg ggctggcggc attaaccagc
     1261 ctgggggcgc tcctttttct ggcgtggagc ctgcgtgaca ttcgcgcgac gcctgacgct
     1321 attccggcct ggccgctggg cggcgtgatc ggctgcgtgg tcttaacttt tgttctgcgc
     1381 ttacaggcgt tcaacacctc tcattacgcg gcttttcatc tggagaacat tctgcgcagc
     1441 aggttagccc gtaaagcatt gcagcttccg cctggcgtgt tacagcaaat gggcagcggg
     1501 tcagtggcga aagtgatgct ggatgacgtg aagtcgttac atatttttgt ggccgacagc
     1561 acgccgctct atgcccgcgc gatcatcatg ccgctggcga caatcgttat cttgttctgg
     1621 ctggactggc ggctggcaat cgcgacgctg ggggtactgg cgtttggatc ggttattctc
     1681 gtgctcgccc gccagcgttc ggaaaatatg gctcagcgtt atcataaggc gcgcgagcag
     1741 gttagcgcgg cggttattga gttcgtgcag gccatgcccg tggtgagaac gtttgatagc
     1801 ggcagcacca gttttttgcg ctatcaacgc gcccttgaag agtgggtcga tgtgctcaaa
     1861 acctggtatc gcaaagccgg tttttcagcg cgtttttcct tctcgattct gaatcctctc
     1921 ccgaccctgt ttgtcctgat ctggtcggga tacggcctgt tgcactatgg cagtttcgat
     1981 tttatcgcgt gggtggccgt tttactgctg ggcagcggaa tggccgaagc cgtaatgcca
     2041 atgatgatgc tcaataacct ggtcgcgcaa acgcgtttaa gcattcagcg tatttatcag
     2101 gttctcgcga tgccggagtt atcgctgccg cagtctgacc agcagccgca agaggcgagc
     2161 attacctttg agcaggtgag cttttattat ccgcaagcgc gtactggcgc cgcgttgcag
     2221 gaggtgagct ttcatgtgcc tgccgggcaa attgtggcgc tggtcgggcc aagcggtgcc
     2281 ggaaaaagca ccgtggcgcg cttgctgctg cgttacgccg acccggacaa aggccatatc
     2341 cgtattggcg gcgtggatct gcgtgatatg cagacggaca ccctgatgaa gcaactctcg
     2401 tttgtgtttc aggacaactt cctttttgcc gacacgatag ccaataacat tcgtctgggc
     2461 gcgccggata ctccgctgga ggcggttata gcggcggcca gagtggcgca ggcccatgat
     2521 tttattagcg ctctgccaga aggttacaac actcgagtcg gggaacgtgg ggtatttctc
     2581 tccggcggcc agcggcagcg cattactatc gcccgggcgc ttttgcagga tcgccccatc
     2641 ctggtgctcg atgaggcgac ggcgtttgct gacccggaaa acgaagcggc gcttatcaag
     2701 gcgctcgcgg cggccatgcg tggccggacg gtcatcatgg tcgcgcatcg tctctcaatg
     2761 gtgactcagg ccgatgtgat attgctgttt tccgacggac agctcaggga aatggggaac
     2821 catacgcaac tgttggcgca gggcggcctg tatcaacggc tctggcaaca ctatcagcag
     2881 gcgcagcatt gggtgccggg tggaacacag gaagaggtgg tggaaaatga aagacaataa
     2941 tcctgcggat aacctggcct ggcgcgtcat ctggcgccag cttatctcca gcgttggcag
     3001 tcaggccagg atgctgcgac gcagtatgct ggcgctgttg ctggcggcat tcatgcaggg
     3061 gatcgccttt gcctgtcttt atccgatcat tgatgcgctg ttacggggag acgcgccgca
     3121 acttcttaac tgggctatgg ccttcagcgt cgccgcaatt gtgacgctgg tgctacgctg
     3181 gtatggcctg ggctttgaat accgtggtca tctggcgcag gctacccatg agttgcgcct
     3241 gcgacttggc gagcagttac gccgcgtgcc gctggagaag ctccagcgcg gcagggcggg
     3301 tgaaatgaac gccttgctgc tgggcagcgt ggatgaaaac ctcaattatg ttattgcgat
     3361 agccaatatt ttgctgctca ccattgtcac gccgctgacg gcgtcgctgg cgacattgtg
     3421 gatagactgg cggctggggc tggtgatgtt gctgatcttc cctctgctgg tgccgtttta
     3481 ttactggcgc cgcccggcga tgcggcgaca aatgcagacg ctgggggaag cgcaccagcg
     3541 tctgagcggc gatatcgttg aatttgctca ggggatgatg gtattgcgca cctgcggcag
     3601 cgatgccgat aaaagccggg cgctgctggc gcatttcaat gcgctggaaa acttacagac
     3661 ccgcactcac cgtcagggcg ctggcgcaac gatgctgatc gccagcgtcg tggagttggg
     3721 cctacaggtg gtggtgttat ccgggatcgt ctgggtagtg acgggcactc tgaacctcgc
     3781 ctttttgatt gccgccgtcg cgatgattat gcgcttcgca gaaccgatgg cgatgtttat
     3841 cagctacacc tcggttgtgg aactgatcgc cagcgccctg caacggattg agcagtttat
     3901 ggcgatagca ccgcttcccg tcgcagagca aagcgagatg ccggaacgtt acgatatccg
     3961 ctttgacaac gtcagctatc gctacgaaga aggcgacggc tacgcgctta atcatgtttc
     4021 tttgacgttc ccggcagcca gtatgagcgc gctggtgggt gcctccggcg caggcaaaac
     4081 tacggtcacc aaactgttaa tgcgctatgc cgatccgcag caagggcaga tttctattgg
     4141 cggcgtcgat attcgccgcc tgacgccgga acagctcaat agcctgattt ctgttgtttt
     4201 ccaggatgtc tggctgtttg atgacacgct gctggcgaat atccgtatcg cgcgcccaca
     4261 ggcgacgcgg caggaggtag aagaggccgc ccgcgcggcg cagtgccttg agtttatttc
     4321 ccgtcttccg caaggctggc tgacgccaat gggagagatg ggcggccagc tatcgggcgg
     4381 cgagcgccag cggatttcca ttgccagagc gttattgaaa aacgcgccgg tcgtcattct
     4441 cgatgaaccg actgccgcgc tggatattga aagcgagctg gcggtgcaaa aagcgatcga
     4501 taacctggtt cacaaccgga cggtgattat catcgctcac cgtttatcca ccatcgccgg
     4561 ggccggaaac attctggtga tggaagaggg acaggtggtt gagcagggta ctcatgcgca
     4621 attgctctca catcatggac gttatcaggc gctgtggcag gcgcaaatgg ccgcgcgcgt
     4681 gtggcgcgac gacggggttt ccgcgtctgg agagtgggtg catgagtgat gttcagtcga
     4741 atgtgaaacc gctgacgttg acgaccgggc gggtgatttt tgctattgcc ggcgtctatg
     4801 tgacgcagag tctggtatcg gcgctgtcta tgcagtcctt acccgcgctg gtgcgcgctg
     4861 ctggcggttc gctggcgctt gccggtgcga caaccctgtt tatgctgccc tgggcgctga
     4921 agtttatttg ggcgccgtgg atcgagcgct ggcggcttcc gcccggtagc caggaacgcc
     4981 gttcccgcat gttaatcctg cgtggtcagg tcgcgctagc ggcgatcctg actattgtcg
     5041 cagcgattgg ctggtttggg cgagaagggg gattccccga tacgcaaatc gtcgcgttat
     5101 ttgttctgtt tatggtggca ggcacggtcg cctccaccat tgatatcgcc agcgacggct
     5161 tttgtgtcga tcaactgacc cgcacgggtt acggctgggg aaacagcgtg caggtcggcg
     5221 gcagctatct gggaatgatg tgcggcggcg gggtgttcct gatgctgtcg gcggcatccg
     5281 gctggcctgt cgccatgctg atgatggcgg tgctgattat ggcgctgtca ctcccgctgt
     5341 ggcgcattac ggagccgacg cgaacagcga ctatcccgca tgttccggcg ttaggttatg
     5401 cgctaaggag gaagcaggct cgcctgggct tactgctggt attgatgctg aattcaggca
     5461 tgcggtttgt gctgcctctt ctggcaccgc tgttgttgga tcatgggttg agcatgtccg
     5521 cattgggcgc gctgttcagc ggcggcaata ttgcagcggg catagcagga acgctggccg
     5581 gcggattgct gatgaaatac acctcacccg gcagagcgct gttgacggct tatggcgtcc
     5641 aggggatcgc gctgctggcg gtggtgatga ctctcatgat ggcgccgggt catctgctgc
     5701 tgcagattct ccagtgtctg gtccttgtcc agtccatttc gctggcctgc gcgctggtct
     5761 gtctttacgc cacgctgatg tcgctttcat cgcctttgca ggccggtgtc gacttcaccc
     5821 tctttcaatg tactgatgcg gcaatcgcca ttctggcagg cgttatcggt ggcgttgttg
     5881 ctcaacattt tggctatgcg gcctgcttcc tgtttgccgg ggcattcacg ttgctggcgg
     5941 cgtgggttgc ttatatccgg ctgcattcgg caagagaact gatgacaagc gcaattgatt
     6001 gatccgggaa taacacagag aggtaaggga tgaaaatcag tgaatttcta catctggcgt
     6061 taccagagga acaatggcta ccgacgattt ctggcgtttt acgccagttc gcagaagaag
     6121 agtgttatgt ctatgagcgt caaccctgtt ggtatttagg caaagggtgc caggcacggc
     6181 tgcacattaa tgccgacgga acgcaggcga catttattga tgatgccggg gagcaaaaat
     6241 gggcggtgga ttccattgcc gactgcgcgc gtcgttttat ggcgcatcct caggtgaaag
     6301 gacgtcgggt atatggacag gttgggttca actttgcggc gcatgcgcgg gggattgcct
     6361 ttaacgccgg ggagtggccg ctgctgacgt taaccgttcc ccgtgaagaa cttatttttg
     6421 aaaagggaaa tgtcaccgtt tatgcggact ccgccgacgg gtgccgacgt ttgtgcgagt
     6481 gggtaaaaga ggccggtaca acgacgcaga acgcaccact ggcggtggat accgccctca
     6541 atggtgaggc atataaacaa caggttgcgc gcgccgttgc ggagatccgc cgtggcgagt
     6601 atgtcaaagt gattgtctcg cgcgccattc ccctgccatc gcggattgat atgcccgcca
     6661 cgctgttata cgggcggcag gcaaacacgc ctgtgcgctc gtttatgttc cgtcaggaag
     6721 gacgcgaagc gctgggcttt agcccggaac tggtgatgtc agtgacgggc aataaagtgg
     6781 tcactgaacc gcttgcgggc acccgcgatc gcatgggaaa cccggagcat aataaggcga
     6841 aagaggcaga actgctgcac gacagtaaag aggtgcttga gcatatcctt tctgtcaaag
     6901 aagctattgc tgaactggag gctgtttgcc agccgggcag cgtggtggtt gaagatttaa
     6961 tgtcggttcg ccagcgcggc agcgttcagc atctggggtc tggcgtgagc ggtcagcttt
     7021 cggaaaacaa ggatgcctgg gatgcgttta ccgtgctgtt tccgtcgatt accgcctcag
     7081 gtatccctaa aaatgctgct ctgaacgcga ttatgcaaat tgagaagacg ccgcgagagc
     7141 tttattccgg cgcaatcctg ctgctggacg atacgcgctt tgatgcggcg ctagttctgc
     7201 gttccgtatt tcaggatagc cagcgcagct ggatacaggc gggggcggga atcatcgcgc
     7261 aatctacgcc ggaacgcgaa ctgacagaaa cccgggagaa attagcgagc attgcgccct
     7321 atttaatggt gtaggagtaa ctgttttcta acctcacaac cagactgtcc aaagacaatt
     7381 gagtcgctct ttgattcctc tgtgactcta ttgtcttctt ttgggataaa ataggtgttt
     7441 ttcgtcatga agttgataaa agtctatata gaaccacctc ctgtggaggt ggtggtaatt
     7501 tgccagagtg agggattcgt tagtatacta tgggataaac ttatttttac caattgtata
     7561 aggcggtgca taagattctc ggcatgcttc cagataatcc gaccaccact gcatcatcgc
     7621 tttgcgggct tctaggtgct ctgccttatg aatataagcc atgcgcacgg tattgcgctc
     7681 ctgatgactc atctggcgtt ctactgcgtc ctttgcccat aaacccgatt ccatcagcgc
     7741 actgcatgcc attgcccgga agccgtgacc gcagatatcc tttttcgtgt cgtaacccat
     7801 cacccgcagt gccttattga ccgtgttttc acacattggc ttatacgggt tatggtcgcc
     7861 ggggaagatc agttcattat taccggtgat atccttaatc tgtttcagaa tggcgatgga
     7921 ctgttctgag agggggacga tatgcggcat tcgcattttt gccccgcggc cggaataacg
     7981 cacgccaata atgggttctc gcgtcgcggg tatcgtccag actcggtttg tgaaatcaat
     8041 ctctgaccag cgggcgaaac gcagttcact ggagcgaatg aacacatgca gcatcagcag
     8101 aacggcatgc cgggtcagtt cacggccctg atgatatgcc ccaatacgtt caagcagttc
     8161 aggcagccgc tccagcggca gggcaggata gtgccgtctg acaggaggtg tggtcacgcc
     8221 gccaaggttt gctgcagggt tcgtatcgat taactcctga tggaccgcat ggcgcattat
     8281 gttactcagg tgctgccgtg tgcgggacgc aacctccagc agaccttttt cctcgatccc
     8341 tttcagcagg tcaatgaaat gacggggttt aagttctgat acaggtaggt tcccgatgac
     8401 cggaaagatg tgattgttca ggctggcaag cagacggtcg gcggtgttct gcgaccattt
     8461 cctgttactt ttatgccacg ccagcgccaa gtttttaaaa actttctccg gtgttcgtga
     8521 gccacgttca gcagcccgct gctgtaccgg gttgatattc agcgccagca ttttacggat
     8581 accttcacgt tgctgtcgcg catcagacag ggagatggct tgataggcac ccagcgcaat
     8641 gcgggattct ttaccgctaa tacggtattt gagataccag tggcgggagc cacccggctt
     8701 gaccagcaga tacagaccgt gagaatcgga gactttaaag ggtttatcag aaggcttgag
     8761 ggtgcggatt tttgcgtcgg taaggaacat atggggggca ctccattatc gaactaacct
     8821 gaccccagat ttgaccacca atttttcccg atgcgtaggg taaaatcaaa atgcaccgag
     8881 aagagttttc acactaacct gttgaatcaa catcagataa agatttgcaa ggatgcatga
     8941 aaacagaaaa ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg
     9001 ccgttctacc gactgaacta cagaggaatc gtgtgaacgg ggcgcatatt acttagcggt
     9061 accttgtctg tcaacactaa atttcataag ccatttcaat tggttaatta atctgcaaag
     9121 ttgttaatta atgaacattt gtcgccgaaa acgagtctgt atcattagcg tgatgcagtc
     9181 tctgcaaagg atcttgttga taaaattgac agaaacgttg ccacaatgaa gggaaacgag
     9241 gagcaaaaag ttctggggcg ctaaagaaat attcagaaag tacggcaaaa cattcagcag
     9301 gatcactggc agcataagca tcaatgctcg ccgcattctc accaactaat tcgatttctt
     9361 cctgaatgtt gctcattgca gcatgaagat cgtgttccca gccagcaacc tcacgcaacg
     9421 aaataaaggg aactccgctg gcgcgatcgc cgttacgggt gtccagctta tgagcgactt
     9481 catgaataat caggttaaaa ccagatgcat caaaagaatc ttgtatatcc aaccagttca
     9541 aaacgatagg cccttgctgc cagctctgac ctgactgaac aatacgttgg ttatgcacca
     9601 gaccgatatc gtcttcccat tcatcatcga ccacaaatgg cgcaggataa attaagactt
     9661 catgaaaacc atccagccat tccagtccta actccagaac gggtaggcaa aatagaagtg
     9721 ctatccggca gcttcttaat gaatccagtt caaagccctg taaaggaaca agccgctttt
     9781 gctgtaaaaa acgttcggca agagtgacta atttgctttg ttcctgttcc gtcagacccg
     9841 ttaaaagggg gatcgatagt gcttcctgcc agggaagggc agtttgatgt gctgattctt
     9901 gtactttcca gggccactta atcatcgttt tgctcgcaaa ctcgtcactt gaacaaaatt
     9961 gcacggacag ggactgttaa aatgccaaat ttcctggcat catggcaacc atctgaacgg
    10021 agagatgccg gagcggctga acggaccggt ctcgaaaacc ggagtggggg caactccacc
    10081 gggggttcaa atccccctct ctccgccaaa attcaatcac ttatacatca ttaagtcagt
    10141 gacaaaaatc acacttggaa ttacttggaa tattttcttg gaatattttc aggtaacggg
    10201 acatcaagtg ttggtgaaac tttaaccttc cggtcataga ttagcacttg cccttcggtt
    10261 ttgtgaccag agaaaagttg cttatcccgg ctgcttcctt catagtctga aattcctttc
    10321 gccttcagat catgaaaggt gaagtcggtt aaaatacctg aaactttgcc tgcgcgattt
    10381 cttgcttcta cccacatttc gttaaagcct ttgtacatat atcggttgcc gtattgattg
    10441 ctgatcacat aggcggattt tggtaactgt tttgcttttt cgaccgctgc ctgtaatcgt
    10501 ggactccatg cttttatctg ttttttccct gttttccctt gctggataaa gatcccgtcg
    10561 tttccaatct gctcccattt cagcgataac acatcggaaa ccctcgctgc acacagatag
    10621 gcaatttcca ttgcgataaa aacaggaagg ggggccacgc ttaatactgc ctggtattct
    10681 ttgttggtta catatcgttc gcggtttttg gccttgaatt tacttactcc tgcacatggg
    10741 ttagccttca cgtaccctcg ctcatacccc caactgtaaa cacgggacat actgcttttt
    10801 tcatggttgg cttgcgtttt actctgttcc cctcttttgt ccatgtatcg acggatgtgt
    10861 tctggtttta tggaatccgc tggtacctta ccgaatacgg caagcaactt tttttgatgt
    10921 tgcagataat ctttttgtgt tcttggacta aggtcactgt aacaggcgct ggcgaggaat
    10981 ttttcccaca agcgaccgaa tgtcattgca cgatcgcgat tatttacagt ttcctcatac
    11041 tttttccata aagcagctaa accatccttg atggcggtta gtgttacaga ttctctggat
    11101 gttggtttcc atacataact atatttattt gggtatacat ttggaggtaa tttttcgtgt
    11161 tcaggatttt tccttcgtct tcccatcaga tcgcaccaaa attcggctct acctcgcgtg
    11221 gtggtaaagt tttattgcag gtaaatagat cccggctgac aatcggtttg ccactacgat
    11281 tggtatagaa cggaagcccg ttttccatta accattttcg ctggtggctt gcatatttgc
    11341 agcccgttaa tattagcaat tcatcttcgg ttaaaaataa gctgctcata gctatatctc
    11401 ataaccgccg ctaactatat gcggttagcg gcgatcaggg ttgaacatta aaaatcagcc
    11461 tgattcggga tcagtttttg tatagtggct gttacgtatt ttgcctggtg aagagcgtca
    11521 tccagagcat tatggcgcac gccttcgaat ggaattacgc ttctggcatt gaagtctgtg
    11581 attttcccca tctcaacgat tgtgcgtaca tcacgatcgt tgctgtaatg ccacggacag
    11641 gggatgtcca gacgttcgta tgaggtgcgc aaaattgcgt tatcgaacgt tgcgccatta
    11701 ccccacacct gaacgaattt ttcatcagag tgttcattga cgaattgccg aaaacgcaaa
    11761 agggcatctt tcatttttac ctggtcagtt aaaatggcag ctctggcttc gctggactgc
    11821 ttcagccacc attcgatggt gccaccatca ggaacagccc ctgtattcat tgcgtcagtc
    11881 aggctgataa cgatataaaa taccggcccg atttcccctg tttccgggtc gaagaaaacc
    11941 gcaccaataa ctactatggg tgcattggtg ttggctccca ttgtttcaag gtcgactgac
    12001 aggtgattcc atactctgct ggtggatgtg atttcacgat gaccgttcat cttaattaag
    12061 gaattttctg tttcgccagt tttattatcg ctggcgtggc cctgagcgcc gccagtgttc
    12121 tccttgtgcg gatgctcagt gccttccgtt tcctccggat ttttttcctg agattcagcc
    12181 tgattttctc cattgaatgt ttcctggtat gttgcgtcac ccattactga gccacaatca
    12241 gggcagttgc cgccgctggt ctgaccgcag gcggtgcaga ctttttccgg ttcctgttgc
    12301 gctactggct caggttgttt cgtttctggc tcgttttgta acgcattttg gctgttttgt
    12361 tccgctttct ggtcgttctg ttccgtttct tgctggttct ggttcactga atcgcgagtc
    12421 tggactccct taacccattt cagatcattc gggtcgctaa tcccttcaac gaactcacca
    12481 cgcgaggccg ccagtaattt gtctgcatcg acaggatttt tgggcggaat gtttttccta
    12541 gcttcatgga gttctgcccg cagtttctga tatttcgcat caacagaatt cacctgcggc
    12601 tgagcatcca gcggctgcgt gtccggatga tgttcagttg tatccggttc cactgtttca
    12661 gtcgttggct gttcatctgc cattgcgcca gacggttgcg gtttttcttc atcatcctgt
    12721 tttccttctt ctgttacacg ctgcggcatt ggggcagagg aacgaccgca ggcaatatcc
    12781 acgatttccg gatcagggtt ggcatgatcg gtttcggtca gcactttatt caggtattcg
    12841 gtgacgcgct ggggcatagc ctctatacca accggtgctt ctttcacgga ggcaaccacg
    12901 atggcgcggg aataatccat cccgcctggc atgttcagaa aagccttaag gaagacggag
    12961 aaacagggat cattttctgc gataatttcg ttaacacgtc tggtatgtga aggatgcatg
    13021 ttgtaaatat ccacatccat cgaacgagcc agttttcctg tagcaacatc gaatgccatt
    13081 gacgcattat catgcacaaa accttcgccg cgatcggtaa ggataccgcc gccagcgtta
    13141 gcgccggatg gtgtgcgact gatgcgagaa acacgatttc ctttcatcca ctcttttgta
    13201 agcagaccac gatcagtgta gtcagcatcc aggtatgctt cgatgaagga ggtcatcagt
    13261 cccaggtctg aatttacggg ggcgggaaaa actttgtcag tgtcccgcac cagtttgtac
    13321 aaatcccgaa tctccagcgg ttcgagttgc gctgctttat ttgagatggc cagcgcggta
    13381 acggcgggaa gtttttcttc ctgtgcgtta tgtaatgagc gcagttcatc ccgcgaaacg
    13441 tgcgttactg gtttttcgcc gccgtgttgc gccagccagc gaacaggcag aatctgacct
    13501 gaaaccggca gaagcatatt ctcctcaatc tcagtcatgt cttcgccatt aacattggtg
    13561 ttgtcagtgc tggctggttt gtcctgcgcg gatggcggag gcgcgataaa tatcattgtg
    13621 atgccatctt cgccgccttt ttcataccgg ttacagaatt cggtatcaaa cacgccttcc
    13681 ggtggaaggt catcaacaac gggcaaatgg acgcgaacag gttttttaaa gtcgtcttca
    13741 tcataatcgt tatcatccat tgcggtaatg cagcgggaga tggcaacaga taattttttt
    13801 gctgtagtcc agtaaaaacc acctttaatc ccaaggcgtt ttctgacttt gtcattttta
    13861 gcttcgcaat atagcgcaaa agtttcttta tcagtgctca ttgataaacc tcattgctgt
    13921 tttaaggttg tgcgaatccc tgccattgct ggcatataag aatcgaccct tatgtattta
    13981 ttaagctgaa tgtcgtattg ggtcagttat ttattacagc tcaccatgcc tccgccttta
    14041 caggtaaacc atcacgaccg aggaagactt taatcatgcg gtcagtaatg catgtttttg
    14101 tagtgaaatc acggatataa agtttttgct tttcgatatt gtttgccgag gcgatatatg
    14161 tccggccttc gtaaataacg taatcgccgg gagtgacgca ctggcgtggt atttcatcgg
    14221 ttccgaagtg atgagcaatc ataataatct ccttaataat ttgtgatatg aaggaaattc
    14281 cagaaaacta tttaatactc agcaactgct cgacggtcat atttttaatt gcgccccggt
    14341 ttatgagagt ccatccctgc ttttccagat aaaaacggaa agtatccaga gtacagacca
    14401 gtgcgccatc aggaactgtt tcggtgaatt tgatgtagcc gtgttcgtcg aagcggataa
    14461 ccagggtgcg gccatcaccc gggattattt tgtcagcggg tgtggtgtta ttccggcgca
    14521 gttcttcctc catgcggtcg aactcggcaa tgtaggcttc cttgaatgca gcggcctttt
    14581 tgcccgtgaa gcccatcaca aggaaaacga agccgttttt ggtgatttgg tacattggga
    14641 gtttgcgtcc tgttgagtcg gtgtattcgc tcgccttaaa attaagggca gtgaactcag
    14701 cggagcagtc cagtaatttt attttttgga taacattgtc gtgacgcttg ccaaagaatt
    14761 ccgcgatcgc aacagacgta gtgacagcgc gaccattttc aatggttacg tcagggtgag
    14821 aaagggtaag ggtagtagcc ataatggcag cctccacgtt gagtattgat aactcaccac
    14881 cgaggctttc cacgaccata atggtggtga gacgtacagg ggtggaaata ccggtcaacg
    14941 tagaacccgg ccagccttgc ggctgccctg cacgccccac cataatctga atgtggctgt
    15001 gcttcacgca taaaaaaacc gcctgagcgc ggttgtgcgc tacgtttgac ttcgggtttc
    15061 cacgcccggc acccggttta tgaggtgcag gtgcactata attccacccg tactggtttt
    15121 caatagctac attcaacatt ttctcttacc tttcatcacc gaagtgaact ttgttgatgc
    15181 ggtgccaggt gcctccaggt gacgttaacc agttaacaat taacgccgga tacagagaat
    15241 ccacccataa ctctgttttt ggttttaact gttccacgtg cgatcagccg cattcaccac
    15301 atcacaaaat tcactttaaa aagggcggca gagcagtcac ggagtaaaac tgataccgcc
    15361 aaacgtcacc agaaaattga taacagatgg cgttgcagcg gggttgtcac tcaggcgcat
    15421 ggtcaacctg acaacccggt gtcctcaagg ggaagg
//
LOCUS       DBBGLQ010000102        15414 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.102, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000102 DBBGLQ010000000
VERSION     DBBGLQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.102"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            523..1233
                     /locus_tag="ACIYSB_004165"
     CDS             523..1233
                     /locus_tag="ACIYSB_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5431088.1"
                     /translation="MSTWVTITEAVEITTKAIKQKITPSDIYRHALSGNILLSVYFQS
                     PVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIID
                     TPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGS
                     LLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPR
                     KWRFDATNSELCAERHEC"
     gene            1160..3281
                     /locus_tag="ACIYSB_004166"
                     /pseudo
     CDS             1160..3281
                     /locus_tag="ACIYSB_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3451..4503)
                     /locus_tag="ACIYSB_004167"
     CDS             complement(3451..4503)
                     /locus_tag="ACIYSB_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGC5431089.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKYLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERIFSFSIAENIIVKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKHS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVLESVIVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            4818..6134
                     /gene="shiA"
                     /locus_tag="ACIYSB_004168"
     CDS             4818..6134
                     /gene="shiA"
                     /locus_tag="ACIYSB_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGC5431090.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            6236..7690
                     /gene="amn"
                     /locus_tag="ACIYSB_004169"
     CDS             6236..7690
                     /gene="amn"
                     /locus_tag="ACIYSB_004169"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGC5431091.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            8033..8749
                     /gene="yeeN"
                     /locus_tag="ACIYSB_004170"
     CDS             8033..8749
                     /gene="yeeN"
                     /locus_tag="ACIYSB_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGC5431092.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(9206..9281)
                     /locus_tag="ACIYSB_004171"
     tRNA            complement(9206..9281)
                     /locus_tag="ACIYSB_004171"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9246..9248),aa:Asn,seq:gtt)
     gene            complement(9382..10869)
                     /gene="yeeO"
                     /locus_tag="ACIYSB_004172"
     CDS             complement(9382..10869)
                     /gene="yeeO"
                     /locus_tag="ACIYSB_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGC5431093.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            11030..11105
                     /locus_tag="ACIYSB_004173"
     tRNA            11030..11105
                     /locus_tag="ACIYSB_004173"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11063..11065,aa:Asn,seq:gtt)
     gene            complement(11143..12093)
                     /gene="cbl"
                     /locus_tag="ACIYSB_004174"
     CDS             complement(11143..12093)
                     /gene="cbl"
                     /locus_tag="ACIYSB_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGC5431094.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKSFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(12195..13112)
                     /gene="nac"
                     /locus_tag="ACIYSB_004175"
     CDS             complement(12195..13112)
                     /gene="nac"
                     /locus_tag="ACIYSB_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGC5431095.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEFN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            13439..13514
                     /locus_tag="ACIYSB_004176"
     tRNA            13439..13514
                     /locus_tag="ACIYSB_004176"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13472..13474,aa:Asn,seq:gtt)
     gene            complement(13570..14505)
                     /gene="ldtA"
                     /locus_tag="ACIYSB_004177"
     CDS             complement(13570..14505)
                     /gene="ldtA"
                     /locus_tag="ACIYSB_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGC5431096.1"
                     /translation="MMRRVNIFCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(14643..>15414)
                     /locus_tag="ACIYSB_004178"
     CDS             complement(14643..>15414)
                     /locus_tag="ACIYSB_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HGC5431097.1"
                     /translation="QHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGII
                     VGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLT
                     YVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSD
                     RGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNSRDEA
                     ISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT         4303 a         3682 c         3373 g         4056 t
ORIGIN      
        1 aaataatcat atcaaaataa aaattcacaa cagtgcaaca ttaaaaaata caaccaacaa
       61 acaatcctat atacaaggca catctccaga atataaaagc acagacaaac aacctaaaaa
      121 aaacaacccc atacatataa attattttat aggtaaaata gattatatat tctcatagct
      181 accacaaact atactagcct gaactatatt tattctgctg caatcaatgc atacataaca
      241 caaatatcac tcaggtacac tactcaaacc acgctgggat ttttcctcaa gttataatca
      301 tcaccccgaa aatcattcgg catttactca ttaaatagtc accccatagg cctgtacatg
      361 tttactcaga aatatacatc cttttctctg tcataaaccc tctaattaat cataaataaa
      421 tacttgtgac accaatcttt ttccttaacg gaacgaattg ttgtgtagaa ggagataata
      481 ttatgtcaaa aaaaatatca gccattgctt ataactcatt atatgtcaac atgggtcact
      541 ataacggaag cagttgagat cactaccaaa gccataaaac aaaaaattac tcctagcgat
      601 atttatcgtc atgccttgag tggcaacatc ctactatcgg tctatttcca gtctcctgtg
      661 atacttaaga agatacaaac ttttaatgga aaaataaaat tcaggcagtt tgtgggagac
      721 ctacttgata aactatgcat gcttgacagg gacggtttta tttatggtca aaatttaaga
      781 ctctgtacag aagctagata tatttgtcct gtgcaacaaa ttatagatac ccccctgtta
      841 cgcaaactca accagtttcg tacgtttgtt cgtaatgtcc gcccgggtga tgaactggat
      901 gtcccggcac aagttagtga aaaaaattta accccgccgc cgggtaacag cagtggcaac
      961 cttgagcaac agatagccag tacttcacag ctaattggat ctctgctcgc cgaagatatg
     1021 aacagcgagc aagcggcaaa catagcacgt ggatgggcct cttctcaggc ttcaggggta
     1081 atgacagact ggttaagccg cttcggtacc gcaagaatca cgctgggcgt ggatgaagat
     1141 ttcagcctga agaactcccg tgacggcaac cctcgaaaat ggcgattcga tgcaacaaac
     1201 agtgaactat gtgccgaacg tcacgaatgc tgaaatcacg ctggcagcct cgaaggaccc
     1261 ggtgattgcc gacaataacg atctcacgac actaacagca ccgtcgctga tacagagggc
     1321 aatgcgatag ccaacactga ggtaacattt actctaccgg aagatgtgaa ggcgaacttc
     1381 acgctgagcg atggcgacaa agcggttact gatgccgatg gcaaagcgaa agtcacgctg
     1441 aaaggtacaa aagcaggcgc tcataccgtg acagcatcga tggttggcgg taagagtgag
     1501 cagttggtgg tgaactttac tgcggatacg ctcagtacgc aggttaatct taacgttacc
     1561 gaggacaatt tcatcgccaa taacattgga atgaccaggc tacaggcaac agtgactgat
     1621 ggaaacggca acccagtaga aggtataaaa gttaatttcc gtggaacctc cgtcacgcta
     1681 agcagcacca gcgttgaaac ggatgatcag gttttcgctg aaattcttgt gacaagcacc
     1741 gaggtcggac taaaaacagt ttcagcctct ctggcagata aacctactga agtcatctcg
     1801 cgattactga atgccaaggt agatgttaat tctgcgacga ttaccagtca ggagatacct
     1861 gaaggtcagg tcatggtcgc acaagacata gcagttaaag ctcacgttaa cgaccagttt
     1921 ggcaacccgg tgacgcatca acccgcgaca ttcagtgcag cgccatcctc gcaaatgatc
     1981 atcagccaga atacggtctc tactaatacg cagggagtag ccgaggtcac catgacgcct
     2041 gaaagaaacg gctcgtatac tgtaaaagca tccctagcga atggagcttc acttgagaaa
     2101 caactggagg ctattgatga aaaactgaca ctcacgtcca gtccgcttat cggtgtcaat
     2161 gctcctaaag gcgctactct gacggcaatg ctaacctctg caaatggcac tccagtggag
     2221 ggtcaggtca tcaactttag cgtaacgcta gaaggtgcga cgttaagtgg cggaaaagtg
     2281 agaaccaact cttcaggtca ggctccagtc gttctgacca gcaataaagt cggtacatat
     2341 acggtgactg catcgttcca taacggcgtc acaatacaga cacagacaac cgtgaaggtt
     2401 actggcaacc caagcaccgc acatgttgct agctttatcg ctgaaccgtc gactatcgcc
     2461 gccaccaaca gttatttaag taccttaaag gcaacggttg aggatggcag tggtaacctg
     2521 atcgaaggtc tcactgtgta cttcgcctta aaaaacggct ccaccacatt aacgtcatta
     2581 acagcggtga ccgatcaaaa cggaatcgcg acaacaagcg tgaaaggagc gataacgggg
     2641 agcgtcactg taagcacagt cacaagcgca ggtggaatgc aaacagtaga tatatcgcta
     2701 gtggcaggcc cagcagacgc ctcgcagtcc atccttaaga acaaccagtc atcactgaaa
     2761 ggggacttta ccgatagtgc tgagctacat cttgttctgc acgatatatc aggcaatccg
     2821 atcaaagttt ctgaagggat ggaatttgtg caatcaggta caaacgtgcc ctatatgaaa
     2881 attagcgcaa ttgattacac tcaaaatatc aacggtgatt acaaagctac tgttacagga
     2941 ggcggagagg gtatcgcaac gctgatcccc gtattgaatg gtgttcatca ggcgggcctg
     3001 agtaccacga tagaattcat tagcgcggaa accagaccaa tgaccggtac agtgtcagtg
     3061 aatggtgcaa acctaccgac agcttcattc ccttcgcagg gatttaccgg ggcgtattat
     3121 cagttgaata atgacaactt tgcaccagga aaaacggcgg ctgcttattc gtttcaaact
     3181 cggcctcttg ggtaggcgtt gatgctactg gtaaagtgac attcaaaaat gacggtgata
     3241 gcaatactgt aataatcaca gcgcccccaa gatccggtgg tgctatttac caaacagttc
     3301 cgcatgaatc gcgttcggtg tgactatgat cattttcggt gagtattgct ttgatccaaa
     3361 ggctggtttt ctgtgatgat tgaccagcct cagagtattg atgacaccac cgttcataac
     3421 agatcggcat tactatgcat aagtactgcc tcaaattctc gccaaaacac tttttaatgc
     3481 ctttgcgaat tcatccagac ttatattgtc aattttttgc ccaacgatga ctgactccaa
     3541 aacatcggcc attgcgggcc atcgcgtttt gtaatcagca taaattgcaa caacaggggt
     3601 attataggca cttgccatat gtaatattga ggtgtcagga gttattgcaa tataagcatc
     3661 ttttatgatt gctgctgaat gtttaataga gggtgataag gataaacggt agacattatt
     3721 ataacaatcc actaatgccc tggctttatc ttcccctttg ggtccataaa cgatcactat
     3781 tggaatatct gtttcacttt gaatttttac tattatattt tctgcaatcg aaaatgaaaa
     3841 tatacgttcc tgcgagctac cttctaaatt gagcgcaatg taagatccta acgatcgcat
     3901 ttcctcgcgt acctcatcga gtacatcatc gcttaatggc aattcatata ttgacctgac
     3961 tgccggaaaa cccgcctcac gcatcataaa tgcccatgtc atgggtacag gagcccggag
     4021 attctgatcc atccgggacg cgttcttaca caaaggggaa tagcacttca tggttaaacc
     4081 gacgacctgg aaattcgttt ttgctttcaa ctgactgata aatatcatcg ttttcaggtt
     4141 ctttttacgc atcgcctcta tacaaagatc cggcgtaccg tattgcagtg ttatgttctt
     4201 cgctaaatat tttatttctt ttaatgttgc gtgatcctgc atagtcattg tgactaatgt
     4261 taatttagtc tgttcaagtt tgagcgcatt aaagacttct aaattaattg tcgacgtaac
     4321 aattaataga tgcttaattt tatgcaattc aagcgcccga ataacaggaa agatggccat
     4381 agcatcgcca atctgatcgg gaatatgtat gacaacaaag tctgtttttt caatattaaa
     4441 attataagct ttataatcgt agtaactaaa tgcaatacgt ctcaacaatg atactaaaaa
     4501 cataactaac ctcgcctccc tactggttat aatgcaatgc agtctatcag aatcatcagg
     4561 gtacaatttt gtgcatatgc ggacttttat gtttcatatc tcgaacctgt gggccctctg
     4621 cttaatcctt aaacaacacc agcaactcct gcgctttcat cttccatcga atttttcatg
     4681 ttgccgctaa tcagccataa agtcatttgc agatgcgctc tgtcgaggta gtctcataag
     4741 gttcgtttat agatcgacgg caatgtgagt taccttttcc atactaatta taaaaagaca
     4801 gtacaaacag gatcattatg gactccacgc tcatctccac tcgtcccgat gaagggacgc
     4861 tttcgttaag tcgcgcccga cgagctgcgt taggcagttt cgctggtgcc gtcgtcgact
     4921 ggtatgattt tttactctat ggcattaccg ccgcactggt gtttaatcgc gagtttttcc
     4981 cgcaagtaag cccggcgatg ggaacgctcg ccgcatttgc cacctttggt gtcggcttcc
     5041 ttttccgacc gctcggcggt gtcattttcg gtcactttgg tgaccggctg ggacgtaagc
     5101 gcatgttaat gctgaccgtc tggatgatgg gcatcgcgac agccttgatt ggtattcttc
     5161 cttcattctc gaccattggg tggtgggcac ctattttgct ggtgacactg cgtgccattc
     5221 agggatttgc cgtcggcggc gaatggggag gcgcggcgtt gctttccgtt gaaagtgcgc
     5281 cgaaaaataa aaaagccttt tacagtagcg gtgtacaagt tggctacgga gtaggtttac
     5341 tgctttcaac cggactggtt tcattgatca gtatgatgac gactgacgaa cagtttttaa
     5401 gctggggctg gcgcattcct ttcctgttta gcatcgtact ggtactggga gcattgtggg
     5461 tgcgcaatgg catggaggag tccgcggaat ttgaacaaca gcaacataat caagcggccg
     5521 cgaaaaaacg catcccggtt atcgaagctc tgttacgaca tcccggtgct ttcctgaaga
     5581 ttattgcact acgactgtgc gagttgctga cgatgtacat tgttactgcc tttgcactta
     5641 attattcaac ccagaatatg gggttaccgc gcgaactttt ccttaatatt ggtttgctgg
     5701 taggtggatt aagctgcctg acaattccct gttttgcctg gcttgccgat cgttttggtc
     5761 ggcgcagggt ttatatcaca ggcgcgttaa tcggaacgtt gagcgcattt cctttcttta
     5821 tggcgcttga agcacaatcc attttctgga tagttttctt ctccatcatg ctggcaaaca
     5881 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
     5941 gttatcgcta cagtggtgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
     6001 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgttg
     6061 ccatttattt gctggctgga tgcctgattt ctgcaatgac cgctttattg atgaaagaca
     6121 atcaacgctc gtgatagcct ggcgaagatc atccgatctt cgccttatac ttttgtttca
     6181 cattcctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
     6241 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
     6301 gcaatctgta gttgctttac gcaacgccat tggcaaatat attacaagtg gcgaattacc
     6361 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
     6421 cggtagcaca actaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
     6481 ctacaccacc acgattactc gccctactct ctttcgttcg tatctcaatg aacaacttac
     6541 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
     6601 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctggttt
     6661 aactcgctac ttcccgacaa cagaactggc gcaaatcggc gatgaaactg cagacggcat
     6721 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
     6781 cctcgcgcgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     6841 gtttaccaac tacacacgtt atgtggatga gttcgttcgt tggggatgca gccagatcct
     6901 cgatcctgat agtccctaca ttgccctttc ttgtgctggc gggaactgga tcactgccga
     6961 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     7021 atggcattta attaccgctg atggtcaggg tattactctg gtgaatattg gcgtgggacc
     7081 gtccaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     7141 gattggtcac tgtggcggat tacgtgaaag ccaggccatt ggagattatg tccttgcaca
     7201 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     7261 gagcattgct gaagtgcaac gtgcgcttta tgacgctacc aagttagtca gcggcaggcc
     7321 tggtgaagaa gtcaaacagc ggttacgtac tggcacggtg gtaaccacag atgacaggaa
     7381 ctgggagtta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     7441 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     7501 actactgtgt gtttcagata aaccgttaca tggtgagatt aagcttcccg gccaggctaa
     7561 ccgtttttat gaaggcgcta tttccgaaca cctgcaaatt ggcattcggg cgatcgattt
     7621 gctgcgcgca gaaggcgacc gactgcattc acgtaaatta cgaaccttta atgagccgcc
     7681 gttccgataa taagttatga accttcttat cagaaataga tgtgaaggat taagtaagac
     7741 atctggcaaa tagcctgcaa taaacgcggg ctgtttgctt ccttgggcag atgcgagttt
     7801 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa acacaattat
     7861 aaaaaatccc gccaacaaca ttagttttta taaaattaaa tataagatta tggctttaga
     7921 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc tgaattaaca
     7981 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     8041 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     8101 tgcaaaattc ggtgtagaaa tctatgctgc tgccaaacaa ggtgaacccg atccagaatt
     8161 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     8221 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tacagggacg
     8281 ttatgaaggc tttggtccta atggctcaat gattattgct gagacattga cttcaaatgt
     8341 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     8401 ggcaggttct gtcagctata tgtttgacaa tacgggtgtg attgtattta aaggaacaga
     8461 ccctgaccat atttttgaaa ttttgcttga agctgaagtt gatgttcgtg atgtaactga
     8521 agaagaaggt aatattgtta tttatactga acctactgac cttcataaag gaatcgcggc
     8581 gctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     8641 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     8701 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat gatattttaa
     8761 agaaatctgt ctttatggca gatttcttta atctcatata attcttataa aaaatataat
     8821 gtttaactcg tcatattgct tataaaaaat ccccgttccc agagaaataa tatttattaa
     8881 aatggcaggt cttctttttc tgattaccga agacaaagtg gcaattacgt atagtttccc
     8941 gataaagacg cgacagctac atcccgcata aggcattttt ctctttatct ttgtacggta
     9001 cttcatggaa cagaattttt gaccctgtga atcgtgatgt ctgttgggga ggggcaattt
     9061 gctcactgaa gcgtgagagt cgattaagcg cacgaaacac agaaatcaaa aaacccggcc
     9121 acttttttta caaagcgacc gggtaaaaat aatttttatt ttttaactgt tttgagactt
     9181 atagagatat ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac
     9241 ggattaacag tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa
     9301 tattagcgac gctcatccac aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg
     9361 aaataatctg cactttgtcg tttattccga cacaactggc tttttttcac acttttgcgg
     9421 ctcaggtcga ggatatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac
     9481 ggcccgcaca gcccagtcgg caaacattcc catccagaca ccaaccacac cccagccaag
     9541 catgattccc agcacataac cgactacaac ccggcaaccc cacatgctca acatagaaac
     9601 ccacatggcg taacgtgcat cgcgagcacc cttaaatcca gcgggtagca cccatgaggc
     9661 agaccaaata ggcataaata aagcatttag ccaaatcaga atcacaacga catgtttaac
     9721 ctgtggatcc tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc
     9781 gatggccgtt aatccaagag tggaaagcca gaacacatgc cgcaactgaa tctctgcttg
     9841 cgctatctgt cctacaccca accttcggcc tgtaatgatc gtagaagcag agccgagcgc
     9901 acttccgggt aagttgataa gagccgcaat tgaaaacgcg ataaaatttc cggcaataac
     9961 actggtcccc atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac
    10021 tgattcgaca ctcgcgggaa taccaatccc catgacttcc cagataatgc taaaattcag
    10081 cggtttaaaa tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac
    10141 ccacaaaatt gccactgcgc caatataacg agaaatggtt aaccccaacc cagccccgac
    10201 aaatcccagt cccggccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt
    10261 aagaatattc aggctaccgt taatcagtag cggtattttc gtattccctg caccacgaag
    10321 tgccccgcta ccaatcaaag tgatggcagc tgctggataa ctgagtaccg tcagctccag
    10381 ataagtcaac gccagtgctt taacttctgt cgtggcatca cccgcgacga aatcaataat
    10441 ttgctcgcca aaatgatgaa taagcgttgc caacagtacg gcaaacaacg tcatgatcac
    10501 caatgactgc cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc
    10561 cacaacgaca gtagtaccaa gatcgatagc agcaaaaaaa gccataatga ccatattgaa
    10621 gctgtccgcc aatcccacgc cggccatcgc atcttttccc agccagctga ccagaaaagt
    10681 gctcagaacc cccatcaaca ggacacaggc attctccatg aagataggaa cagcaagcgg
    10741 ggttatctcg cgccagaaca acactttgta gctcttgcgt ttagcgtgcc agcgagtgcc
    10801 gtgaacaacc tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg
    10861 aaaccacatt tcaaataatg agggagaatc agcaaagctg caaagatttt cgccaacaaa
    10921 ttgtctgcaa atgcaacaaa ctgttgatag aaacggcaaa cagttgggaa aattaaaaat
    10981 cgggtttgac aaaagatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt
    11041 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
    11101 agccaaattt ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat
    11161 ttcctcttca ctgttttcca tcacctgccg cttgatatcc tcaactgaaa gtcctgcatt
    11221 acaaagttcc agaaagcgcc agacatagtt acgctgaagt tgtcctcgct tcagtcccaa
    11281 ccagacagta ttagcatcaa aaagatgccg cgtatccagg cggattaaat tcttttcctc
    11341 ttgttcaccg ctggattgtt cggcaactat tccgatccca agcccaagag caacataggt
    11401 tttaatgaca tcagaatcct gcgcacttaa tacaatatct gccagcaaac ctttgcgggc
    11461 aaatgcgtca tcaatacgtg agcgccccgt aatcccctgt cggtaagtga ttaacggcca
    11521 cttcgctatt gattccagcg tcaatggtgt aatttgcgtc aagggatgat cgagtggaac
    11581 aagcaaacta tggtgccaac gaaaccacgg gaaggcgacg agctgcgggt cattactcaa
    11641 acgctcgctg gcgataccaa tatcagcttc accattatgc aacaacgtcg caatttcctg
    11701 tggcgtcccc tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagatttaat
    11761 gacctctggc aaactataac gtgcctgagt atgcgtcgtt gcaatagtga gaacgccaga
    11821 cgtatcgttg gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg
    11881 ttctgcaatg accagtaatg ctttgcccgg ttcagtcatg cccagcagtc gcttacctcg
    11941 tcgaacaaat atttcgatgc caagttcatc ctccagttcc cgaatatgac ggctgacgcc
    12001 ggactgtgag gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc
    12061 ctcgcggatt atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagattttc
    12121 cgttattgtt aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca
    12181 tcccgatata acgcttagct caccaattgc cactgccttt tttccatcac tggagaactc
    12241 atcactgaca ttaacaactc tttcactgcc tgtgcctgtg gtgataagtt cgctctggcg
    12301 ggtaaattta atgacaaaga gagactcatg gaaggtgtgg taatgcgtga catccacccg
    12361 tttactgcgc cacataacga acgcgcggcc gattcgggta atactgcaac gcccatgccg
    12421 ctggcaatcg ctgcggtaag cgtggcaata gactcaattt caccaatgac ttttgccgtg
    12481 agtcgccgta gggaaaaagc ctcatcaaca cgaagtctaa cagcactgta atcactggga
    12541 agaaagaggt tcatttgcgc aatagcatta acatcaacgc tttgccccgg acaatcttga
    12601 gtccctacca ggaaaagatc ttctttcagt aaagcctgac tggatacacc agccacaggg
    12661 gaatgctcat aaatcaccgc catatcgagt tggtgattta tcagtttttc gttaagcact
    12721 gcaccgctat tttcatgaag atagataacg acctccggga attcagcgcg aaccgcctgt
    12781 aataagggca tggtgatgga tgacgcagcg gttcctggcg caaagccaat cgagacttgc
    12841 cccgataatg cctgaccaac gttatgcacc gccagttggg cctgctcaca ctgacgtaaa
    12901 atggcccgcg catgggtata gagaattttt ccggcgtcag ttggcgtaac gccccgtttt
    12961 gtgcgaatca aaagttgttg attaaactca ccttccagtg tggcaacctg ctggctgagc
    13021 gctggttgtg cgatatgcaa tacttcagca gcctgggtca ggctaccaat atctacaatt
    13081 tttacgaagt atttcaggcg tctgaagttc atgttgcctc cggtttttaa gagtcggccc
    13141 aagtgccgcc attacttaca accagaatgc aagatgcttg ccagttctat tttggcgttg
    13201 atgcgcaagc taaccaactg tcaaataaga gattatgata gattcgtcat tggctctttt
    13261 tatctgcagt cgcattcacc tgccctataa aaggagggta tgcaccacga tggttcatta
    13321 cccaataaga ttgaaagctc accactttgt tgaaattggc agcaaacaaa caaaaaaatg
    13381 catttcaccc tttgacatca ccatgcactg ccattaatat gcgccccgtt cacacgattc
    13441 ctctgtagtt cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc
    13501 agtcagagga gccaaattca aaaaagcctg ctttcgagca ggctttttac tttctaatta
    13561 tcaacgctct taaaacatct gtcttgaacc agaactaatt tgcacaggca ttcccgatcg
    13621 acgttgcaac gcagcatttg cgcgatttac atcaacttct tgcccgttga taaacgcccg
    13681 caaagatggg gttaccggca atggcacttt tcggtcagac tcatattctg cacgattgcg
    13741 cgacagcggc tcatgaactt ccagccactt tgaaccatcc ggttccgttg tgtatttcac
    13801 tggctgatca ataatttgta cacgagtgcc tacaggaaca ttatcaaaca gatatttgat
    13861 atcgtcatta cgcagacgaa tacagccctg acttacacgg agcccaatac caaaattggc
    13921 attggtaccg tggatggcat acaacctgcc aatataaatc gcgtacagcc ccatggggtt
    13981 atcgggcccc gcaggaacaa atgcgggcaa actctcccct cgtttcgcat attcgcgccg
    14041 ggtgttcggc gttggcgtcc aggttggtgc ttcttgttta cgttcaacgg tagtcaccca
    14101 gttacgcggg gtttctcgcc cagcctggcc gataccaata ggaaagactt ccactgtatt
    14161 actgtctggc ggatagtaat aaagacgcat ttcagcgacg ttaaccacaa tccctttacg
    14221 aacagtggcg ggcaaaatca gttgctgcgg aatggtgagt tgtgagccag gcttcggcaa
    14281 aaaaacatca gcgcccggat tcgcttccag catattactt aacccttgcc cgtattgtgc
    14341 ggcaaaagtc tccagcggct gagtattgtg atcaggaaca gttacagtaa gcgactgccc
    14401 cactaaacgg ctaccctctg gaggtaatgg ataagttacc gccaggctat tttggctggc
    14461 aaaaagcaga gcaaatgagc aaaaaatatt tacacgacgc atcatgtccc tttcctaagt
    14521 cgcgaaagct atccgttaag tatagctttt atcagacttt tcgtttttaa ctgttcaaat
    14581 cagaagtcgt attccccggt gaggtgtact ggcaatagcg gacactacca tttgttcttt
    14641 ttttaagcag ccatctgatg atatttttcc ctgaaggctg ccggggagat attccccaga
    14701 cgagagtgac gacgctgacg attgtagaaa atctcaatgt attcccgtat tactgagatg
    14761 gcctcatccc ggctattaaa acgatagtgg ctcaggctct catttttcag cgttccccag
    14821 aagctttcca tcggagcgtt gtcgtaacag ttacctttac gcgacattga tgttttcaga
    14881 ccaaactgct cctgtatgac ccggtaatcg tatgcgcagt actgtgaacc tcgatcggag
    14941 tggtggatta gcccggcagg tgggcgctgg ctcctgagcg ccataaacag ggctttacct
    15001 gtcagctctt ttgtcatgcg ctctcccatg gcgtagccga caatttcgca cgtataaaca
    15061 tctttgatgc cagcgaggta caaccatccc tcctgtgtgg caacatacgt caggtccgcc
    15121 acccagacct gatttggtgc tgtaggagcg aacgtctggt tcagcagatt tggcgcaact
    15181 ggcagattgt ggttcgagtt cgtagtcgct ctgaacttgc gtttctgctt acagcgtagc
    15241 cttagctcct tacgaagacg tgccagtcgg tcacgaccaa cgatgatgcc attctctgcc
    15301 agctccgtct ggagccgccg ggttccatat gtttcgcgag tgcggatatg tgccacctta
    15361 atctccagtt ttagccgctc atcactttgt tttctgtctg agggttcatg ctgt
//
LOCUS       DBBGLQ010000103        15348 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.103, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000103 DBBGLQ010000000
VERSION     DBBGLQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.103"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..886
                     /gene="ligB"
                     /locus_tag="ACIYSB_004179"
                     /gene_synonym="yicF"
     CDS             <1..886
                     /gene="ligB"
                     /locus_tag="ACIYSB_004179"
                     /gene_synonym="yicF"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HGC5431098.1"
                     /translation="WWKAELPFVTDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQ
                     VAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPCDQILVS
                     LAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWLGAKQVL
                     GLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQP
                     FTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQ
                     IKKLGSWLAAQQITGFEP"
     gene            complement(883..1500)
                     /locus_tag="ACIYSB_004180"
     CDS             complement(883..1500)
                     /locus_tag="ACIYSB_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGC5431099.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(1791..2615)
                     /gene="dinD"
                     /locus_tag="ACIYSB_004181"
     CDS             complement(1791..2615)
                     /gene="dinD"
                     /locus_tag="ACIYSB_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGC5431100.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGGATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(2836..3699)
                     /locus_tag="ACIYSB_004182"
     CDS             complement(2836..3699)
                     /locus_tag="ACIYSB_004182"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGC5431101.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            3826..4542
                     /gene="rph"
                     /locus_tag="ACIYSB_004183"
     CDS             3826..4542
                     /gene="rph"
                     /locus_tag="ACIYSB_004183"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGC5431102.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQDQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4608..5249
                     /gene="pyrE"
                     /locus_tag="ACIYSB_004184"
     CDS             4608..5249
                     /gene="pyrE"
                     /locus_tag="ACIYSB_004184"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGC5431103.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(5286..5882)
                     /gene="slmA"
                     /locus_tag="ACIYSB_004185"
     CDS             complement(5286..5882)
                     /gene="slmA"
                     /locus_tag="ACIYSB_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGC5431104.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(5989..6447)
                     /gene="dut"
                     /locus_tag="ACIYSB_004186"
     CDS             complement(5989..6447)
                     /gene="dut"
                     /locus_tag="ACIYSB_004186"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGC5431105.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(6425..7645)
                     /gene="coaBC"
                     /locus_tag="ACIYSB_004187"
     CDS             complement(6425..7645)
                     /gene="coaBC"
                     /locus_tag="ACIYSB_004187"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGC5431106.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            7817..8485
                     /gene="radC"
                     /locus_tag="ACIYSB_004188"
     CDS             7817..8485
                     /gene="radC"
                     /locus_tag="ACIYSB_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGC5431107.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            8702..8938
                     /gene="rpmB"
                     /locus_tag="ACIYSB_004189"
     CDS             8702..8938
                     /gene="rpmB"
                     /locus_tag="ACIYSB_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGC5431108.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            8959..9126
                     /gene="rpmG"
                     /locus_tag="ACIYSB_004190"
     CDS             8959..9126
                     /gene="rpmG"
                     /locus_tag="ACIYSB_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGC5431109.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            9224..10033
                     /gene="mutM"
                     /locus_tag="ACIYSB_004191"
     CDS             9224..10033
                     /gene="mutM"
                     /locus_tag="ACIYSB_004191"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGC5431110.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(10072..10551)
                     /gene="coaD"
                     /locus_tag="ACIYSB_004192"
     CDS             complement(10072..10551)
                     /gene="coaD"
                     /locus_tag="ACIYSB_004192"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGC5431111.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(10559..11836)
                     /gene="waaA"
                     /locus_tag="ACIYSB_004193"
     CDS             complement(10559..11836)
                     /gene="waaA"
                     /locus_tag="ACIYSB_004193"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGC5431112.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            12249..13307
                     /gene="rfaQ"
                     /locus_tag="ACIYSB_004194"
     CDS             12249..13307
                     /gene="rfaQ"
                     /locus_tag="ACIYSB_004194"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGC5431113.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHASGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            13304..14428
                     /locus_tag="ACIYSB_004195"
     CDS             13304..14428
                     /locus_tag="ACIYSB_004195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGC5431114.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFTWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGGDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            14421..15218
                     /gene="rfaP"
                     /locus_tag="ACIYSB_004196"
     CDS             14421..15218
                     /gene="rfaP"
                     /locus_tag="ACIYSB_004196"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGC5431115.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEIFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLLQAEAKATKIRERTIRKSL"
BASE COUNT         3794 a         3713 c         4034 g         3807 t
ORIGIN      
        1 gtggtggaaa gcggaattac ccttcgtcac cgatggcgta gttgtacgag cggcgaaaga
       61 gccagaatcc cgccattggc taccgggcca ggcagagtgg ctggtggcct ggaaatatca
      121 acctgtagct caggttgccg aagtgaaggc aattcagttt gcggtgggta agagcggtaa
      181 aatatcggtg gttgcgtcac tcgcacctgt catgctggat gataaaaaag tccagcgggt
      241 gaatattggt tccgtcaggc gctggcagga gtgggatatt gcgccttgtg atcagattct
      301 cgtcagcctt gccggtcagg gtattcctcg cattgatgat gtggtgtggc gcggtgcaga
      361 acgtacaaaa ccgacaccgc cagaaaaccg ctttaactcg ttgacctgct actttgcttc
      421 tgatgtttgt caggaacagt tcatttcacg cttagtctgg ctgggggcaa aacaggttct
      481 tgggctggat ggcattggtg aggccgggtg gcgcgcgctg catcagactc atcgctttga
      541 gcatatcttt tcctggcttt tattaacgcc agagcaatta cagaacacgc cggggatcgc
      601 gaaaagtaaa agtgcgcagc tatggcatca gtttaatctg gctcgtaagc agccttttac
      661 tcgctgggtg atggcaatgg gaataccgct aacccgggcg gcgcttaatg ccagtgatga
      721 acggtcctgg tcgcaacttt tatttagcac ggagcagttc tggcagcagc tgccgggcac
      781 tggatcgggg cgcgccagac aggttattga atggaaggaa aatgcgcaaa tcaagaagct
      841 gggcagttgg ctggctgccc agcagatcac aggttttgaa ccttagtgtc cttcgtggct
      901 gtagtaaaag accggtaatc ccagcttaag gcgtagcgcc agtaagcggg caaaaaagcc
      961 gaataccagg gtggagataa ttaccacatc atgattagaa acatagtgtt gcagcgcgat
     1021 gtacagcacg gcggaggcaa atgagacacc ggcatacaac tctttctgga ataccagtgg
     1081 gatgcgttta cagaacatat cgcgcaaaac gccgccaaac acgccggtgg ttaccgccgc
     1141 gacaacggca ataattgggc cgtgccccat atccagtgca acctgtgccc cgatgataga
     1201 aaagaccacc agtccgagcg catcgagcac cagaaacact ttgcgcaggt aaggcattac
     1261 aggagcaacg atagtggtga gtactgcggc ggtagcgacg ataatcacat attccgggtg
     1321 tttgacccag ccgagcggat agtggcccag cagaatatcg cgcactgacc ctccgccaat
     1381 tgcggtggcg gtcgcaataa taattacgcc aaatgtatcc atgcgccgtc gtccggccgc
     1441 cagcgccccc gtcatggctt cggcagtaat gccaaccaaa tacaaaatgt gtaacagcat
     1501 ggttctctcc ctaaaaatct gtctggcagg gtagcgattt gtgcgcattg tcacgattga
     1561 gattttttaa ggtgagggga aattgataac ttgtagttat gtgaaatcgc tattttctgt
     1621 agcagagatg cattcttctg acttcttgtc tgagaaaatg cataagaaaa aataggttaa
     1681 aactgagggg ggaaaagaaa ggtgcaatat ttagccattg tgttcactgc acctggacct
     1741 tggcttacgc atcgggtgtc tcaatcaaca aaaagagttt ttagtttctg ctatttcttc
     1801 tctgtaattt ttacactgtt ttccaactgc ttgatgctga cctgcggggt cggcaactcc
     1861 tcaggcatgg ttccgccaag ttcctgaatg gtttgcctca ccttgcgacc cacgtcaaag
     1921 tgtgtggtgt ttgcttgctg ttttgaattc acgccatccc gcttgagttt ttcttctgtt
     1981 tgggtagctc gaaagagatt agccgccagt tctgttgaac ccatatgatc caggatcttc
     2041 tgattctttt tcagcccctt ccgctgatga atagctttct gatctaatcc accatacagc
     2101 ccccggtaac catgattctg aaatatggcg aaatcgatag ctgtcgctcc cccagcttgc
     2161 tgcgctgcct caaccaattg tttgttatgt tctttcaatt catttctcag aaaaagacgc
     2221 ttttcgtctt cacgaagttg cctgaaagct tcatcgtcag ccagctcctg ccgtcgggtc
     2281 tggatagcaa aataagtttg ccctgccgca ataaccggtt tcgcagggtc gccgttttgt
     2341 actaccaggt aacaggcata gcgggagaga tgaacatctt caagttctcg ttgagcccct
     2401 gaacctaata caaccatttt ggtggtttca acgaaatggt cagatgcagc ttgattgctg
     2461 gcttcgcacg cttgagtcgc gcgggccagt accttttgaa aattgcgcca gtcccgataa
     2521 tccagtaacg gtgccaattc tctagctgac caaaattctt gcccttcagt gccataatgc
     2581 cgtatttctt caaaaggttg atgatgttcg ttcattgccc actccatgtg atatcgattg
     2641 caaagaaagt aaatctgtaa ctgtatttat atacagttgt gagtatctca cgttctcagt
     2701 gtgctgggca aaaaaacatc gcaaaatctg gagcaggcac gcaaaacaga attgaagttg
     2761 cggggaagaa aaatgccgct ctgtttgagt gggcggcatt tgtgaaggct gaaaagtaag
     2821 aattagtgtt acgagttact caatattctg aatctgctcg cgcatctgct caatcaacac
     2881 tttcagctcg atggcggagt ttgtcacttc ggcattgata gatttcgacg caagagtgtt
     2941 cgactcgcgg ttgaattcct gcatcataaa gtccagacga cgaccaacgg cttctttttt
     3001 cttcagaatg ttgtaggtct ctttgacatg cgcttccagg cggtccagtt cttcggcaac
     3061 gtcaattcgt tgtgccagca gaaccaactc ctgctccaga cggttgtttt ccaactgaac
     3121 ctgagcatct tccagcttcg cgaccagacg ctcacgctgc cattgcagga tttccggcat
     3181 atgggcgcgg actttgacca cttcggcggt gacgccttcc agacgctgct cgatcaatgc
     3241 tttcagtgcc tgaccttcgg tttcgcgcgc gacaataaag tcgtccagcg taccatccag
     3301 cgccgcgaga atttcagcgg caatggcgtc gagatcctgc tcctgggctg ccatcacgcc
     3361 cggccagcgc agaatatcaa ccgggttgat ttccccttcg tcactctgca tttttaccca
     3421 gttcgcggca gttaccagct gtttagccag tttttcattg aggatcagct caccttgtgc
     3481 gctaacatct ggctcatagc gcagggtaca ttccacttta ccgcgcgtca ggcgagaacg
     3541 aatacgctcg cgaacgacag gttcaaggct acggaactgc tccggcagac gaaagtaagt
     3601 ttccagataa cgctggttta ccgagcgcat ttcccaggtt gcgctccccc attcaccctt
     3661 gatttcacgc cgggcgtagg cggtcatact gcggatcata gacgttcctg tttataaaag
     3721 gagaggtgga aggattatag ccatcgatgc cttgtcagga taggaataac cgccggaagt
     3781 ccgtataatg cgcagccaca tttgtttcaa gccggagatt tcaatatgcg tccagcaggc
     3841 cgtagcaata atcaggtgcg tcccgttacc ctgactcgta actatacaaa acatgcagaa
     3901 ggctcggtgc tggtcgaatt tggcgatacc aaagtgttgt gtaccgcctc tattgaagaa
     3961 ggcgtgccgc gcttcctgaa aggtcaggac cagggctgga tcaccgcaga gtacggcatg
     4021 ctgccacgtt ctacccacac ccgtaacgct cgtgaagcgg cgaaaggtaa gcagggtgga
     4081 cgcacaatgg aaatccagcg tctgatcgcc cgtgctcttc gcgcggcagt agatttgaaa
     4141 gcgctgggtg agttcaccat tacgctggac tgcgacgtgc ttcaggctga tggtggcacg
     4201 cgtaccgcgt cgattacggg tgcctgcgtg gcgctggcag atgcgctaca gaagctggtg
     4261 gaaaacggca agctgaaaac caacccgatg aaagggatgg tagccgcagt ttctgtcggt
     4321 attgtaaacg gtgaagcaat ttgcgatctg gaatacgttg aagactcagc cgcagagacc
     4381 gacatgaacg tagtgatgac cgaagacggg cgcatcattg aagtgcaggg gacggcagaa
     4441 ggcgagccgt tcacccatga agagctactc accttgttgg cgctggcccg agggggaatc
     4501 gaatccattg tagcgacgca gaaggcggcg ctggcaaact gatttttaag gcgactgatg
     4561 agtcgccttt tttttgtctg tagaaaagta agatgaggag cgaaggcatg aaaccatatc
     4621 agcgccagtt tattgaattt gcgcttagca agcaggtgtt aaagtttggc gagtttacgc
     4681 tgaaatccgg gcgcaaaagc ccctatttct tcaacgccgg gctgtttaat accgggcgcg
     4741 acctggcact gttaggccgt ttttacgctg aagcattggt ggattctggc attgagttcg
     4801 atctgctgtt tggccctgct tacaaaggga tcccgattgc gaccactacc gccgtggcgc
     4861 tggcggagca tcatgacctt gacctgccgt actgctttaa ccgcaaagag gcaaaagacc
     4921 acggtgaagg cggcaatctg gttggtagcg cgttacaagg acgcgtaatg ctggtagatg
     4981 atgtgatcac cgccggaacg gcgattcgcg aatcgatgga gattattcag gctaatggcg
     5041 cgacgcttgc tggcgtattg atttcgcttg atcgtcagga acgggggcgc ggcgagattt
     5101 cggcgattca ggaagttgag cgtgattaca actgcaaagt gatctctatc atcaccctga
     5161 aagatctgat tgcctatctg gaagagaagc cggaaatggc ggaacatctg gcggcggtta
     5221 aggcctatcg cgaagagttt ggcgtttaaa gaaactcgcc ggatgaaaag tcatccggcg
     5281 tcatattact gcaactgcgc ggcaattagc ggccagcggg cgtcaaaatc atccgtcggg
     5341 cggtatttaa attcgctgcg gacaaaacgt gacagcatac cttcacagaa ggccaggatc
     5401 tggcttgcca gcagggtttc atcggtggtg taaccttcac cctcacgcat tctcttttca
     5461 cgcaatacct gacgcagctg cgcttcaata cgctcgaaca gctggttgat acgcccttgc
     5521 aggcgatcct gttcaaacat cagcgcatga ccagtgagga tgcgggtcag gccaggatta
     5581 cgctcaccaa aaccgagaag cagcaacaca atcagacgca ggcgcgctgt ggtgtctttc
     5641 tcatctttca gaatcaggtt gatgcgagta atcaggctat cttcgataaa ctcaatcagg
     5701 ctatcgaaca tgcgggtctt actggggaag tggcgataca gtgccgcttc ggaaacgccg
     5761 acagaggcgg ccagttttgc cgtcgtgata cgttggcttc catcgctgga ttccagcatc
     5821 agcgccagag actgaagtat ttcctcgcga cggttccttt tcgcagtttg tttttctgcc
     5881 atgttacaaa atacccctga aaataagcac ttgccaggcg gcacccacgc tatgaccgca
     5941 aacgaaatgt ttgcggctat gttatgacgt tattcggatg cgtatgtgtt actgacgacc
     6001 agagtgacca aagccgcctt caccgcggtc ggtggcgtcg aaatcttcca ccagattaaa
     6061 ttcagcctgt actaccggaa caaaaatcat ctgggcgatg cgttcgccag gttgaatggt
     6121 gaagctgtcc tgaccacggt tccaaacgga aatcatcaac tggccctgat agtcagaatc
     6181 gatcaatcct accaggttac caagcacgat accgtgctta tgtcccaatc cggagcgcgg
     6241 cagcatcatt gccgccagtg aaggatcggc aatatgaatc gccagcccgg tcggaaccag
     6301 cgtagtgtca cccggagcca gttctacggc gtcgtcgaga caggcacgca ggtcaagtcc
     6361 ggcagagcca gaggtggcat aagtcgggag cggaaattcc ttcccaacgc gcgggtccag
     6421 aatcttaacg tcgatttttt tcatcataac gggtcacgat ctcgtcgagt aataattggc
     6481 caaggagctc tttgcgctca agcggtaaga ctttatctcc gtcctgccag aaaaggtgta
     6541 atgcgttgtt gtcgctgtta aatccttgag ttggctggga aacatcgttc gcgcagatca
     6601 gatcaaggtt tttacggata cgtttttgcc gggcgtattc ttccacatta tttgtttcgg
     6661 cggcaaatcc aacgacgtag ggtcgatggt cttttagtgc ggcaacgcct gcgacgatat
     6721 cggggttttt aaccattttt attgttaatt catcaccctg cgtggcctgc tttttgattt
     6781 tttctggggc cacggtagct gcgcgataat ccgccacggc ggcgcaaccg ataaaaatat
     6841 tttgctgctg tacagaagca ttcacggcgg cttccatttc cagcgcggtc atcacatcaa
     6901 cacgtttaac aaacggtggc gtcggtagtg aaaccggacc tgataccagc gtgacgttcg
     6961 cgccacgacg ggcagcggcg gcggcgatag caaaacccat cttgccggag ctgtgattag
     7021 agatataacg caccggatcg agcggttcac gcgtcgggcc ggcggtaatc ataatgttca
     7081 gatgtttcag gtcgttgacg ggcgaaaaat gcgctaccgc catatccaca atggttaatg
     7141 gatcgagcat tcgccctggg ccgatatcac cacaagcctg actgccactg tctggccccc
     7201 agatcagcaa accacgggaa gcaagcacct ctaaattatg ctgcgtggca gcggcacggt
     7261 acatctgctg gttcatggcg gggagcacgg ctacaggcgc aggtgtagcc agacaaatcg
     7321 tcgataccag gtcattcgcc attccggcag caacacgggc aatcaaatct gccgtggcag
     7381 gggcgagaat cactaaatca gcccatttac ccagctcaat atggcccata gcggcttcgg
     7441 ctgccgggtc cagcagactg tcggaaacgg gataaccaga aaccgcctgc aagctaagtg
     7501 gggtgataaa ggcttttgcc gcttcggtca tggctacgcg gacgtcggcc ccgcgatcgc
     7561 gcaaacgacg caccagttca ggggttttat aggcagcaat accgccgcta acgccgagaa
     7621 cgattttttt accggccagg ctcatgatga tttttcctgt tgggtgacac cagaagttgg
     7681 cgattttatc acaatacttt tgttgtcgtg ccttcacctg agattcactt tgcgaggctc
     7741 tttccaggat tgaaaactgg ccgtcgattt aacggaacga ctatgacagg atgcgagcac
     7801 cacaaaggag gtgaaggtga aaaacaatgc acagctgttg atgccgcgcg aaaaaatgct
     7861 gaagtttggt attagcgcct taacggatgt cgagctgctg gcgctatttc tgcgtaccgg
     7921 aacgcgcggt aaagatgtat taaccctggc aaaagagatg ctggagaatt tcggctctct
     7981 ttatggcttg ttaacctctg aatatgaaca atttagtggt gttcatggaa ttggcgtagc
     8041 gaaatttgcc cagttaaagg ggattgctga actggcgcga cgttactaca acgtgcggat
     8101 gcgtgaagaa agccctttac tcagcccgga gatgacgcgg gaatttttac aaagccagct
     8161 cacgggtgag gagcgggaga tctttatggt gatctttctc gactcccaac accgggttat
     8221 aacgcatagc cgtctttttt ccggcacgct aaaccatgtt gaagtccatc ctcgggaaat
     8281 tatccgcgaa gcgataaaaa taaacgcctc ggcgctgatc cttgcacata atcatccttc
     8341 gggttgtgct gaacccagta aagcggataa actcattact gaacggataa taaagagttg
     8401 tcagttcatg gatttacgcg tgctcgacca tatcgtgatt gggcgtggag agtatgtttc
     8461 ttttgccgaa cgcggctgga tttaacccgc tatgcgcgat ccttcgggat ctttgtctgt
     8521 tcgggacttg agcacatcgc tgagtcagcg tatactacgc cacctttgag aatctcgggt
     8581 ttggcatttg ggcctggcaa tcgagagttc acatagaact gcgatgaccg ggctgtaaag
     8641 cctgacgagg cgccaatacc ccatacgaag ctcgagctaa tttgattttt ggagaataga
     8701 catgtcccga gtctgccaag ttactggcaa gcgtccggtg accggtaaca accgttccca
     8761 cgcactgaac gcgactaaac gccgtttcct gccgaacctg cactctcacc gtttctgggt
     8821 tgagagcgag aagcgttttg tcaccctgcg cgtatctgct aaaggtatgc gtgtaatcga
     8881 taaaaaaggc atcgatacag ttctggctga actgcgtgcc cgtggcgaaa agtactaagt
     8941 acttagagga aataaatcat ggctaaaggt attcgtgaga aaatcaagct ggtttcttct
     9001 gctggtactg gtcacttcta taccactacg aagaacaaac gtactaagcc ggaaaaactg
     9061 gaactgaaaa aattcgatcc agttgttcgc cagcacgtga tctacaaaga agcgaaaatc
     9121 aaataattct cgctttgatg taacgaaaaa cctcgctccg gcggggtttt ttgttatctg
     9181 cttaccccca tattgattgc atctgttcat tcctggagat gctatgcctg aattacccga
     9241 agttgaaacc agccgccgcg gcatagaacc gcatctcgtt ggtgcaacca ttcttcatgc
     9301 tgtggtgcgc aacggacgct tgcgctggcc ggtttcagaa gagatctacc gattaagcga
     9361 tcaaccagtg cttagcgtgc agcggcgggc taaatatctg ctgctggagc tgcctgaggg
     9421 ctggattatc attcatttgg ggatgtctgg cagcctgcgc atccttccag aagaacttcc
     9481 ccctgagaag catgaccatg ttgatttggt gatgagcaac ggcaaagtgc tgcgctacac
     9541 cgatccgcgc cgctttggtg cctggctgtg gaccaaagag ctggaagggc acaacgtgct
     9601 ggcccatctt ggaccggagc cgcttagcga cgatttcaac ggtgagtacc tgcatcagaa
     9661 gtgcgcgaag aaaaaaacgg cgattaaacc gtggctgatg gataacaagc tggtggtagg
     9721 ggtaggtaat atttatgcca gcgaatcact gtttgcggcg gggatccatc cggatcggct
     9781 ggcgtcatca ctgtcgctgg cagagtgtga attgttagct cgggtgatta aagcggtgtt
     9841 gctgcgttcg attgagcagg gtggtacaac gctgaaagat tttctgcaaa gtgatggtaa
     9901 accgggctat ttcgcgcagg aattgcaggt ttacgggcga aaaggcgagc cgtgtcgggt
     9961 gtgcggtacg ccgattgtgg cgactaaaca tgcgcagcgg gcaacgtttt attgtcggca
    10021 gtgccagaag taattcatgc gcgccggatg gcataccatc cggcataaac gctacgctaa
    10081 cttcgccacc agcgcctgat ggacattctc cggcaggaaa tgggtgacat cgccctgatg
    10141 gcgcgccacc tctttcacca acgatgaaga gataaacgac cactctttcg acggcatcag
    10201 aaacacactt tccagttccg gcattaagtg gcgattcata tgcgccagct gcatttcata
    10261 ttcaaaatcc gccaccgcac gcaggccacg aatcagcacc gtagcgtgtt gattacgggc
    10321 gaagttcgcc atcaaatcac taaacccaac cacttccacg ttccccagat gcgcggttgc
    10381 ctgctgtgcc agcgccacac gctcttccag ggtaaacatc ggttttttac tggggctggc
    10441 ggcaatcgcc agaataacgt gatcgaacat ttgcgtggcg cgcgtcacga tatcgatatg
    10501 accattggta atgggatcga aagtacccgg ataaatcgcc cgtttttgca taacaacctc
    10561 aatgcgtttt cggtggcagg taaggttcca gcagttgaag cagacgctgt agcgcgccct
    10621 ggttttgata cagtacttca acggcatgac ggccatagaa actacggtaa tcggcgtcgg
    10681 tgagtaaaga ggaaacctct tttgcaagcg tagtggcatc ggtaacggta atcagcccgc
    10741 ttgcctgctc cagccgcgcg caaatgtctt taaagttaaa agtatgcggc cccatcaata
    10801 ccggaatagc gtgtgcggca gcttccagcg gattatgccc accacgttca accagtgaac
    10861 cgccaacaaa ggcgagatcg gcaatgccgt acagtaacat caactcgccc atcgtatcgc
    10921 caaccacaac ctgcgtgctg gtggagggga cttcccctga agagcgtgtg atatagctta
    10981 gtccagcctg gcggacaagg ttaatcgcat ccgggaagcg ttccggatga cggggtacca
    11041 ggatgagcaa taaattcggg aattgctgta acaatgcctg atgtgcggcg atcaccacac
    11101 tctcttcgcc ttcgtgagtg ctggtggcaa tccataccgg gcggtgtggt gcccactggc
    11161 ggcgcagcgt cacggcttta gcagccaact gcggcgttac agaaatatcg aatttcaggc
    11221 taccggtaac ggtcacctga ttattttttg cgcccagcgc cacaaaacgt gcaccatctt
    11281 cttcattttg cgcagcaatc agcgtaatac gacgcagcaa gcgacggacg aatttaccca
    11341 gtttggcata acctgcggcc gagcgggcag agagtcgcgc gttagcgatc accagcggaa
    11401 ttttacgttt atgtagcgcc gcaatcaggt taggccatag ttcggtttcc ataatcaaca
    11461 ccagtttagg gtcgacttta ttcaggaaac ggttgagtgc atcgggcaga tcatacggca
    11521 gataaacgtg ctgaacatcc ttcccgaaag ccgattgtac gcgctccgaa ccggttggcg
    11581 tcatggttgt tacggtaatc ggtaaatcag gataacgatg acgcagcgcg cgcaccaacg
    11641 ggattgccgc cagagtttca ccgacggaga cggagtgcag cataatgccg cctggtttta
    11701 gcggatggcg gtaaaaaccg taacgttcac cccagcgttt tcgataggcc ggagccttac
    11761 gtccgcgcac ccagagccgt atccagatca gcggctgaat aaggtagaga agggcggtgt
    11821 aaagcaattc gagcatagta aatagctgac ttatggatgt gctggggatt ctatgtattt
    11881 agctgtggct ttaccattac ttttcccgtt tttgacttaa atagcaattt acccgccaat
    11941 tcttgctgaa catctatact ttttttgtat ttttatgcga ttcattgatt tctgctgtac
    12001 gtgcaggaag gaatcgcaga gcaacagtga aagaatatgg ttgctaatga ttttatttga
    12061 ttgatttctt tattgaaaat caacaatgtg cttaaaaatc attcgggtcg gattgccgcg
    12121 aaagaagtga tacactagcg cgtcaatatc agtgcggtat cagtattcag gtagctgttg
    12181 agcctggggc ggtagcgtgc tttttctgct tcaccttaat cggataatct caacaaaaag
    12241 agttacttgt ggataagcca tttcgaagaa ttttgctcat taagatgcgt tttcatgggg
    12301 atatgttatt aactactccc gtcattagtt cgctgaaaaa aaattaccct gacgcaaaaa
    12361 tcgatgtgct gctttatcag gacaccatcc cgatcctgtc tgaaaatcca gagattaacg
    12421 cgctctacgg cataaaaaat aaaaaagcaa aagcctcaga aaaaattgcc aacttttttc
    12481 atctcatcaa ggtattacgt gccaataagt atgaccttat cgtcaatctt accgatcaat
    12541 ggatggttgc tatactggtt cgcttattaa atgctcgtgt gaaaatttcc caggattatc
    12601 atcatcggca gtctgctttt tggcgtaaaa gtttcaccca tttggtgccg ttgcagggtg
    12661 gaaatgtggt ggaaagtaac ttatccgtgc tgacaccatt gggacttgat tcgttggtga
    12721 agcagacaac catgagttac ccgcctgcaa gctggaaacg tatgcgtcgc gaacttgatc
    12781 acgctggtgt tggacaaaat tatgtggtta tccaacctac ggcgcggcaa atcttcaaat
    12841 gctgggacaa cgccaagttt tccgctgtga ttgatgcctt acatgctagt ggttatgaag
    12901 tcgttctgac gtccggcccg gataaagacg atctggcctg cgtcaatgaa attgcgcagg
    12961 gatgccagac gccaccagta acggcgctgg ctggaaaggt gaccttcccg gaacttggtg
    13021 cgttaatcga tcatgcgcag ctgtttattg gcgttgattc cgcaccggcg catattgccg
    13081 ctgcagttaa tacgccgctg atatcgctgt ttggtgcgac agaccatatt ttctggcgtc
    13141 cctggtcaaa taacatgatt caattctggg cgggagatta ccgggaaatg ccaacgcgcg
    13201 atcagcgtga ccgaaatgag atgtatcttt cggttattcc ggcggcagat gtcattgctg
    13261 ctgtcgataa attactgccc tcctccacga caggtacgtc gttatgatcg ttgctttttg
    13321 tttatataaa tattttccct ttggcggttt gcagcgcgat tttatgcgta ttgctcagac
    13381 agtcgccgcc cgaggtcatc atgttcgggt ttatacccag tcgtgggaag gcgaatgccc
    13441 tgatgtattt gaactgatca aagtgccggt taaatcgcat accaatcacg ggcgcaatgc
    13501 ggagtatttt acctgggtgc aaaaacattt acgcgaacat cccgtcgata aagtcgttgg
    13561 attcaacaaa atgccggggc tggacgttta ttatgccgct gatgtttgtt atgccgagaa
    13621 agtagcgcag gagaaaggct ttttctatcg cctgacgtca cgttatcgcc attatgccgc
    13681 ctttgagcgg gcaaccttcg aacagggcaa gccgacacag ctgctgatgc tgacagataa
    13741 gcaaatcgcc gatttccaga aacattatca gactgaagcg gagcgttttc atattctgcc
    13801 accggggatt tatcctgatc gtaaatatag ccagcagcca gccaatagcc gtgaaatctt
    13861 ccgtaagaag aatggaataa ccgaacaaca atatttattg ttgcaggtcg gttcagactt
    13921 cacgcgtaaa ggtggcgatc gttccattga agcacttgct tctttaccgg attcgctgcg
    13981 ccacaacaca ttgctatatg ttgttgggca ggataaaccg cgaaaatttg aggcgctggc
    14041 agaaaaacgc ggcgtgcgca gtaatgttca cttcttctcg gggcgcaatg atgtctcgga
    14101 attaatggcg gcggcggatt tattactgca tcctgcctac caggaagcgg cgggaattgt
    14161 gctgctggaa gcgattactg caggattacc ggtactaaca acagccgttt gtggctatgc
    14221 gccttatatt gtcgaggcca attgcggcga ggctattgct gagccattcc gccaggaaac
    14281 attgaatgag attttacgca aagcgttaac gcaatcttca ttgcgccagg cctgggcgga
    14341 aaatgcgcga cattatgctg atacacaaga tttatacagt ctgccagaga aagcggcgga
    14401 tatcataacg ggtggtctgg atggttgaac ttaaagagcc gtttgccacg ttatggcgcg
    14461 gtaaagatcc ttttgaggaa gttaaaacct tgcagggtga gatatttcgt gaactggaaa
    14521 ctcgccgtac tctgcgcttt gaaatggcgg gaaaaagcta ttttctcaaa tggcatcgcg
    14581 gcacgaccct gaaagagata atcaaaaatt tactctcatt gcggatgcca gtattaggcg
    14641 cagaccgcga atggaatgcg attcatcgac tgcgggatgt cggcgttgat actatgtatg
    14701 gggtggcatt cggcgaaaaa ggcatgaatc cgctgaccag aacctcgttt attattaccg
    14761 aagatctgac accaaccata agtctggaag attactgtgc tgactgggcg accaacccac
    14821 cagatgttcg cgtaaagcgt atgcttatta agcgtgtcgc gacgatggtg cgcgatatgc
    14881 atgctgcggg cattaatcac cgtgactgtt atatctgtca tttcctgctg cacttgcctt
    14941 tttccggtaa ggaagaggag ttaaaaattt cggtaattga cctgcaccgg gcgcagcttc
    15001 gcacgcgcgt tccacgtcgt tggcgcgata aagatcttat tggactttat ttttcttcga
    15061 tgaatattgg cctgactcag cgggatatct ggcggtttat gaaggtgtat tttgccgctc
    15121 cgcttaaaga cattctcaag caggaacaag gactgctgtt gcaagcagaa gcaaaagcca
    15181 caaaaatcag ggaaagaacg attcgaaaat cgttgtaacc cgtaacttat ttttgccaaa
    15241 attttggata cagaataaat atgtctcaac tcaatgatag tgacatcatc ctttttgagt
    15301 ataattttca ttatcaaaat ataagatcta aaaatactct tgatatag
//
LOCUS       DBBGLQ010000104        15337 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.104, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000104 DBBGLQ010000000
VERSION     DBBGLQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.104"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..607)
                     /locus_tag="ACIYSB_004197"
     CDS             complement(<1..607)
                     /locus_tag="ACIYSB_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein E"
                     /protein_id="HGC5431116.1"
                     /translation="MLDPADLPPDDILDYVAIDTDKTGHLRVRVVEGKKHLRAVQEYL
                     TRLRARHQGRVGDFEFTTLDVIARLRQDTTTAGDESVINPVQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDS"
     gene            complement(704..1378)
                     /locus_tag="ACIYSB_004198"
     CDS             complement(704..1378)
                     /locus_tag="ACIYSB_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431117.1"
                     /translation="MIFRTIHHKSPHTRDVLLLTLDGIYLMLENKTTDDFIFVKQHIY
                     RIFQWMHETPLINNGNTPVTDRLIADIRWMLEHHYYPSPGQVDRIISVLRETKFQLLR
                     EKTDRQELVEDIQQKLNTLKDIRHFLKLNTNYKQDGDIISLTPSGEWKNTSIILSVVA
                     VMLMIFASVCNLSGITPYFIYTAGIIFYALATPVLTLRSTQPEKNIPLLFAFGGVIIF
                     TLLSLC"
     gene            complement(1396..1950)
                     /locus_tag="ACIYSB_004199"
     CDS             complement(1396..1950)
                     /locus_tag="ACIYSB_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431118.1"
                     /translation="MNISELISWLSLIIRDLETAAAEYGVNHTDIVHEATQLQVQLCR
                     GKQVTPAQLRALSARLWGARMRLAAQYGQDAPLMNDLAFLSNCLKYDADRLNDRWLYR
                     EWISAAESFVLPLVFIIPLLIALCYMMKSGNSGGAELCAALAGAWCTGLTFLYLWAKD
                     PVGLFWSLYSFIPLYLLWCDISPA"
     gene            complement(1999..2451)
                     /gene="pilP"
                     /locus_tag="ACIYSB_004200"
     CDS             complement(1999..2451)
                     /gene="pilP"
                     /locus_tag="ACIYSB_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HGC5431119.1"
                     /translation="MHAKTKALLVSALLLPATAMATTGNAEEQSPRQLQIERIQDETA
                     LYEAMLAREKIRHELRMLEQGIPAEPGRPQESTSAIPTAPSTATSPRLTEIFGVQPQL
                     RARIELSGGHITDVKTGQNIPGTPYLVEKITPDSVVLEQSGIRKTLRP"
     gene            complement(2441..3736)
                     /gene="pilO2"
                     /locus_tag="ACIYSB_004201"
     CDS             complement(2441..3736)
                     /gene="pilO2"
                     /locus_tag="ACIYSB_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HGC5431120.1"
                     /translation="MTDISPTERQVFPLPAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPRYSFVSAVDGVLVSDLVGSGEEVREARDTFLSINTEPEQGWTRYEPVAFSA
                     GDQNQALPLSTLTGSGKHPAAARLNPVSRGAQFITVSLIIALLGAAWYGWQYYERWKT
                     EQAAQAAAARQVTEEQISPPWPGLPETDAFIQGCADIWTSLPLSASGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTGTRPFIVMPEGKTGGIGLPVSFRLPEHPV
                     DVDALPESSVLQEQLTTLAQSMPLTLDWQEVSNSVTDENGNIIQPPWKEYDLQIQTLL
                     PAYQLVERLKAPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            complement(3757..5376)
                     /locus_tag="ACIYSB_004202"
     CDS             complement(3757..5376)
                     /locus_tag="ACIYSB_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HGC5431121.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPSSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVTYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGVVSVVTQHSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            complement(5407..5844)
                     /gene="pilM"
                     /locus_tag="ACIYSB_004203"
     CDS             complement(5407..5844)
                     /gene="pilM"
                     /locus_tag="ACIYSB_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HGC5431122.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQNPERRTAGGRLTSAQLGLPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSHDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            complement(5849..6919)
                     /locus_tag="ACIYSB_004204"
     CDS             complement(5849..6919)
                     /locus_tag="ACIYSB_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HGC5431123.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIITHTQKRLLPPPVTRPVTPPRPTTTATPSTTVSSQSTALPPRPLLQATPLSALVMT
                     GKPGPSSVVYLREARQRTVSDWIRQLLPTGWQFRQQNAATPALNKRLPSWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGASGTPESRTTTPAPVATKPVVPPPQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            7041..7436
                     /locus_tag="ACIYSB_004205"
     CDS             7041..7436
                     /locus_tag="ACIYSB_004205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431124.1"
                     /translation="MIKKMWRYLRQIDKYELLQQVISVHEVELLRKMMTDSLLGCSVG
                     YVFSKILKISFLRFIQSEGGWDIITYFLWMLGMLIVMAVLFIWMCAAVCYELRMYKIK
                     TGRENDILFWVWILLFDVAVVFSLGNVIW"
     gene            complement(7941..8183)
                     /locus_tag="ACIYSB_004206"
     CDS             complement(7941..8183)
                     /locus_tag="ACIYSB_004206"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HGC5431125.1"
                     /translation="MSAEKFRVVIYTNTCEKKVICIPATEDINIAIMPFQTADNALII
                     VCQNTRIIRRTERIPGSCSEWKEVALPLKARSRIKL"
     gene            complement(8244..8417)
                     /locus_tag="ACIYSB_004207"
                     /pseudo
     CDS             complement(8244..8417)
                     /locus_tag="ACIYSB_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
     gene            complement(8460..9125)
                     /locus_tag="ACIYSB_004208"
     CDS             complement(8460..9125)
                     /locus_tag="ACIYSB_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HGC5431126.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEDIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(9266..9907)
                     /locus_tag="ACIYSB_004209"
     CDS             complement(9266..9907)
                     /locus_tag="ACIYSB_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HGC5431127.1"
                     /translation="MCLSELQAGLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHAFTHLRRHSAFLGFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            10609..11481
                     /gene="repA"
                     /locus_tag="ACIYSB_004210"
     CDS             10609..11481
                     /gene="repA"
                     /locus_tag="ACIYSB_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGC5431128.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            12173..12364
                     /locus_tag="ACIYSB_004211"
     CDS             12173..12364
                     /locus_tag="ACIYSB_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431129.1"
                     /translation="MAESGYGLAGLGMGKVKSINQCRLTPGFGGFTLVPYVATLCRLP
                     CRSRGIRTIKPALNVFAKV"
     gene            12437..12688
                     /locus_tag="ACIYSB_004212"
     CDS             12437..12688
                     /locus_tag="ACIYSB_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGC5431130.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            12685..12972
                     /locus_tag="ACIYSB_004213"
     CDS             12685..12972
                     /locus_tag="ACIYSB_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGC5431131.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
     gene            13076..13666
                     /locus_tag="ACIYSB_004214"
     CDS             13076..13666
                     /locus_tag="ACIYSB_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HGC5431132.1"
                     /translation="MMKNPLAYFHIATFLLSGKSRNLSEYWKAEVIMRDKFVLHRLIR
                     NGMQRQRGFLMWWRLANEMFISGNKKQRKCAIKIKNALMERYGCDIGLGARIGKGLVL
                     PHHAGIVIHGNVDIGENVIIRQNTTIGEKVSDSRENYIVIGDNVDIGAHTCIIGLNVK
                     IGSNVKIGAMSFIMEDVPDNCTYVTRKESRVIIREQ"
     gene            13769..14431
                     /locus_tag="ACIYSB_004215"
     CDS             13769..14431
                     /locus_tag="ACIYSB_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGC5431133.1"
                     /translation="MGSVTKKPSPVVIIKKKRRILSHQDDAVQAVPDDIKPAPVMEFK
                     TDSLPPEIPATVAGSPEQQKKTGRKKKKKKNNRWNHEFIRHSLDCVKKTFPQLRAEEG
                     GYHAFKIGISKDVMAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVAGTPRYGLD
                     GKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQPSKTVTPDSNLKDACIHN
                     GA"
     gene            14489..14803
                     /locus_tag="ACIYSB_004216"
     CDS             14489..14803
                     /locus_tag="ACIYSB_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431134.1"
                     /translation="MITVIISEVDGWREWKHRVRTKDAQTAIIRAMNKHFPRSYNFIP
                     DDIDNAPVLFAAVTRTPNVKITGHIWKPMWNRGICWNVKGPPVIITLIQGAAWNSENK
                     PR"
BASE COUNT         3772 a         3575 c         4142 g         3848 t
ORIGIN      
        1 gactgtcatc cttgatcaga cggagcacag catagaggcc gcccacgcag ggatagtggg
       61 agtagcgtgc accaaacaga ccggccagtt tcagatactg ctctgaaaga cgtgcatcct
      121 gaggcacccc gggatcaaac tgtgtgccct tgataacatc ggtcatgccg gaataggtgg
      181 cccccagtaa ttcgaggcct tcttctttcc ggaggatatc gagcacttcc agttcgccgt
      241 gaacccgggc ttccacatag gtgaagtccg tgttgtctcg tcccggggtc atatgaaggt
      301 ctgaggcacc aagagaggca gaatggcgga tatagccgag cattttctgc tgtaccgggt
      361 taatgacgct ttcatcaccg gcggttgtgg tgtcctgtct gaggcgggct atcacatcca
      421 gggtggtgaa ttcaaagtct cccacacgcc cctgatgacg tgcgcgcagg cgggtcagat
      481 attcctggac ggcacgcaga tgtttttttc cctccacgac cctgacccgc agatgaccgg
      541 ttttatctgt gtcgatagcc acataatcca gaatgtcatc cggtggcagg tctgcaggat
      601 ccaacataag gcctccttac gggcagcggc gcccggaaaa tatgttttct gttattaagc
      661 gagggaaata atcagttctt ctgcggagag aatctggaca ttttcagcac aggcttaaca
      721 gtgtaaaaat gattacgcct ccaaaagcga ataacagggg aatgtttttc tccggctgtg
      781 ttgatctcag cgttaatacc ggtgtggcga gagcgtaaaa aataatacct gcggtataaa
      841 taaaataagg tgtgatgccg gacagattac atacactggc gaatatcata agcatgacgg
      901 caactaccga taaaataatg gaagtattct tccattcacc agatggtgta agtgaaatga
      961 tatcgccatc ctgtttatag ttcgtgttca gcttcaggaa atgacggata tctttcagcg
     1021 tgttcagttt ctgctggata tcttccacaa gttcctgtct gtcggttttt tcacggagca
     1081 gctggaattt tgtctccctc agtacagata taatacggtc cacctggccg ggggagggat
     1141 agtaatgatg ttcgagcatc caccggatat cggcgattaa ccggtcagtt accggtgtat
     1201 taccgttgtt aatgagaggt gtttcgtgca tccactgaaa tatgcggtag atatgttgct
     1261 taacaaaaat aaaatcatcg gtggttttat tctccagcat gagatatatc ccgtcgagtg
     1321 ttaataataa aacgtcccgg gtgtgaggtg atttgtgatg tattgtacgg aatatcattt
     1381 ctgcactgta atcgtttacg caggtgatat atcacaccag agtaaataca gcggaatgaa
     1441 gctgtagagt gaccagaaaa gcccgacggg atcttttgcc cacaggtata agaatgtcag
     1501 tccggtgcac catgcgccgg caagtgccgc gcataattca gcgcctccgg aattgccgct
     1561 cttcatcata taacagagcg cgatcagcag tgggataatg aagacgagcg gcaggacaaa
     1621 ggattcggct gcggatatcc attcccggta aagccagcgg tcgtttaatc tgtcggcatc
     1681 atatttcaga cagttgctca ggaacgccag atcgttcatg aggggggcgt cctggccata
     1741 ctgcgccgcg aggcgcattc tggcacccca caaccgggca ctcagggcac ggagttgtgc
     1801 cggggtgacc tgttttcccc gacaaagttg tacctgtaat tgtgttgcct cgtggacaat
     1861 atctgtgtgg ttcaccccat attctgcagc ggctgtttcc aggtcacgga tgatcaggct
     1921 gagccaggat atcagttcac tgatgttcat tgtcgttcat ccttttgtac ggacagccgc
     1981 ctgtactggc ggccggtttc atggtcgcag ggtttttctg atgccggact gctccagtac
     2041 cacactgtcc ggtgtgattt tctccacgag ataaggggtt ccggggatgt tctggcccgt
     2101 cttcacatcg gtgatgtgtc cacccgaaag ttctatccgg gctctcagct gaggctgtac
     2161 accaaatatt tctgtcagac ggggggatgt cgccgtcgac ggggcagtcg ggatggcgga
     2221 cgtgctttcc tgcggacgac cgggctctgc gggaataccc tgctccagca tgcgcagttc
     2281 atgacggatt ttctctctcg ccagcatggc ttcatacagg gctgtttcgt cctgtatccg
     2341 ctcaatctgc agttgccggg gactctgttc ctctgcgttt ccggttgtgg ccattgccgt
     2401 tgccggtaac agcagcgctg aaaccagaag ggcttttgtt ttagcgtgca taatatttac
     2461 ctgtaaattg atagtggaaa cgaccgttct ccagttgtcg tgtgacagag ataaaacgga
     2521 cagaaggcgc cttcagacgt tcaaccagct ggtatgccgg gagtagcgtc tgtatctgaa
     2581 ggtcatactc tttccacgga ggctggataa tatttccgtt ttcatcggtg acgctgttac
     2641 tgacctcctg ccagtccagc gtcagtggca ttgactgagc aagcgtggtc agttgttctt
     2701 gcaggacaga gctttccggc agagcatcaa cgtcgacggg atgttcaggc aggcggaagc
     2761 tgactggcag gccaatgccg ccggtctttc cttccggcat cacaataaag gggcgggtac
     2821 ccgtcatatc actgatgcgc cggatgaagt cagtcactgt cgcgccagct gtattagtgt
     2881 acgaggcgcg aagattgccg ctgccgccgt cagttgaaca ttcagccagc gaataacgcc
     2941 atccggaggc agacagtggc agtgacgtcc atatgtcagc acatccctgg ataaaggcgt
     3001 cggtttcggg gagtccgggc caggggggac tgatttgttc ctctgtgacc tgtcgggcag
     3061 ctgctgcctg cgcggcttgt tcagtcttcc accgttcata atactgccag ccgtaccatg
     3121 cagcgccgag aagggcgatg atcagcgaga cagtgatgaa ctgcgctccc cgggataccg
     3181 gattcaggcg cgcggcagcc ggatgtttac cagagccggt cagggtggac agcggcaggg
     3241 cctgattctg atcgcctgct gaaaatgcca ccggttcata gcgtgtccag ccctgctccg
     3301 gttcggtgtt gatggaaaga aaggtatccc gggcttcacg gacctcttct ccgcttccca
     3361 ccaggtcact gacgagaaca ccgtcgacag cggaaacaaa gctgtagcgc ggtgtttctc
     3421 cgggtaacgt cagttccgtg atggcatagc cgccatgttc aagcagtggc aggatcagca
     3481 gggcgaggga gaagcactga cggggttttc cgggcagtat gcggcgactg acagtggcta
     3541 ccatggtgtg ggtatcggca tcttcattac tgagatactg acaggccagg aaaaggtcgg
     3601 ctttctgtgc ccgggcaaag tcgcgggcat gccggtactg tgagggcagt gttttccatt
     3661 ccagcccggc cagaaacaca ttgcgccctg ccggcagcgg aaacacctgc cgctctgtcg
     3721 gtgaaatatc cgtcacgatc gtcctccttg tgaaaatcag atgatattgg gcgtgatcag
     3781 caccacgaga atggtgtcgt cgttttcact gttgatccca ccgccaagac cgaagaatcc
     3841 cggcgtgcct acaccctgtt ttccgctgcg acgtcctgac tgctggaagc cactgataat
     3901 gaccgtctgc ccggatttca gatccacggt ctgattgatg gttttcagat ccacattcgg
     3961 cagctgggct ttacttccac cgctctcata attctcaata accggtttgt cggagagact
     4021 catggaatag agcaactgca gtgtctgtga gtccgggaga atgaacggca gaagggtcat
     4081 gttaaaaccg gtggtgatgg tggccgcatt cagggaggtg gatgagccga cattggcagt
     4141 ggtatccgtc gtcacactct cgatgtaact ctgctgattg gcgatctgca ttggcaccgg
     4201 cgtcaggttt ttggtgacgg cagagtgctg ggtcaccaca ctgacaaccc cctgggaaga
     4261 caatgctttc aggaaggctt ttgaaccggt gagtttaccg tccacaatgg aaacaccacc
     4321 tgtgatggca tcactggctg cactggtgaa tgtgctgccg agactcagtc ccagcgttct
     4381 gtcgctgaag actgcgttcc agtcgatacc ggcctgctca ttgcgggttt tccggatact
     4441 cagaatttcc acattcagca ccacctgacg tttcatctct tcattgcgtt tttcaatgaa
     4501 tgcccggacc gtttccagaa cctgtggatt atctgtgacg gttataaagc ccgtggacat
     4561 gaacatgcgt ccgatacccg gggtcagcat tgatgtgacc gcatttttca catcctgata
     4621 cagcgcagac ttcatatcca cggtggtggt ctgggtattg ctggcatcac cggaaatggc
     4681 atctgatgca ctgcctcctg atgagccgtt tgaggacatg gtgccgctga ccacagtggc
     4741 gttataggtc acctgactgt ccatataggt gatggggaag gtctgtgtct cgagataata
     4801 gaagatgata tgcgagccgt catagcgcca ggatataccg agacgggagg agaccgcatt
     4861 cagcagggtc gtcaggccgg aggcattcag ttcagacagt gctgcggtat ttcccggcag
     4921 acgggatgat ggcgtgtcag atgcgccaat ggcggcaagg ggaaccatgc cgcttgcatc
     4981 cggtgggggt aacgcgccct ggatttgttg tgtgctgccg gttgtggatg tattcaggta
     5041 tgtcagtaca tcaggtgtga tccgtaccgg aatatggcag ctttccgtga tccgctgaac
     5101 cacctgctga agggtgatgc tctgacgggt atgaatgaca atgggacaac cgggaacggt
     5161 tttgccggtg tttttatcca tgaccggacg gggattgatc cattgtcgag aggattcctt
     5221 aacaaccggc gtccgggttc gcagatcaga catcatttcc cgtgcccgtt gttgttccgc
     5281 aacggcttcc tttcttgtgc tctcaagctg tctgtaactg gcacagccct gtaacagagc
     5341 ggcggcaatc ataatggcaa gaattttttt attcatggga gatccgtgag ttattcagat
     5401 tcgtgactaa ttcatccaga ggatgacgtg atcggggatc accgacggaa gtgtgacgga
     5461 aagcgctctg ccggcggtgt ccagcagccg gccgttttcc accctgccga ggagggcaga
     5521 gtcatgactc tgttccctga gggcggccat caggcccggg gtttctgtgg cccagacaaa
     5581 aacacgttgc tggctgatga tatgactgac ggtatccgtc ccgggaagcc cgagctgagc
     5641 ggatgtcagc cgccctccgg ctgtccgtcg ttccggattc tgatgcaggt aatcattgat
     5701 ggcgttcata taacgaatga acgttgctgc ctgttgtgtg ttttctgcgg cctgatgacg
     5761 gtgttcgctg cgggagagct gactggtgaa ccagaaattg ccggtggtcg tgatgaccat
     5821 gatgaacacg atgaaccagc ccataaggtt attcctgtgg acgatctgtc acccgcagca
     5881 gacattgtgc tgtgctggtc cgggcataca gcggtttttc caccggggtg tagagtgcaa
     5941 atagctgttc cagcgcctgg cggaaactgc cggtgaaaac cagaggagca tcgatgctgt
     6001 agttgacagg tgtttcccag cgcagtgtcc atgtgccgtg cgggcatttt tcggatgatg
     6061 tccagcgggc cagtccgtct ttcagggtgg tccccttttc cattttccat tgccgtggcg
     6121 gtatgacctt ctgcggtggt ggcactaccg gtttcgtggc caccggggct ggcgtggttg
     6181 ttctgctctc tggtgttccg gatgccccgg tggtggtctg agcccgggat acgctgacgg
     6241 caggtgtcac tggtgtcggg cctgccggcg ttgcggtgct gcccctgaac ggattacgcg
     6301 gtgtatcagc agctttctgt gaaccctgcg gtgccgtgac gtcgaccggc ggtgttgcag
     6361 aggtcgtgac agtcagtatt ttcctgttcc agtccatatg tccgtaaagc tgctggccgg
     6421 ccagcagcgc attcagtgcc cggtgccact ggtcattaac ggaccaggaa gggaggcgtt
     6481 tgttcagtgc cggtgtggcg gcattctgct ggcggaactg ccagccggtt ggcagcagct
     6541 ggcgtatcca gtcactgacg gtgcgctgac gtgcttcacg taaataaacg acggatgagg
     6601 gaccgggctt accggtcata accagtgcag acagcggtgt ggcctgaaga agcgggcggg
     6661 gaggcagtgc ggtactctgg ctgctgacag tggttgaggg tgtcgccgtt gttgtcggac
     6721 ggggcggtgt cacagggcgg gtcaccggtg gcggaagcag acgtttttgc gtatgggtga
     6781 taatggcagc actttcgcga atttgtccgt cgacgaacac cagagccgga ggcgtggtat
     6841 ccggaacagg cgtggcatta aatgccgtac agccgctgag taatgacggc agaaggagtg
     6901 tcagtgattt cttttgcata gaatataatc cggtatatta cgggttacgg tataagacag
     6961 gtggcgggta actggccttc agatgaatat catttcatcg ggttattgat gatgaacctt
     7021 aaaaatcaca gaggtgatgc atgataaaga agatgtggcg ttatttacgg caaattgata
     7081 agtatgaatt gctgcagcag gtgatttcag tacatgaagt ggaactgctc aggaaaatga
     7141 tgacagattc attattaggg tgctcagtag gatatgtgtt ttcgaaaata ctgaagatat
     7201 cctttctgcg ttttattcag tctgaggggg gatgggatat tattacttac tttttgtgga
     7261 tgctgggaat gcttattgtc atggccgtgc tgtttatctg gatgtgcgcc gctgtatgct
     7321 atgagcttcg gatgtataaa ataaaaacag gaagggagaa cgatatatta ttctgggtgt
     7381 ggattcttct ttttgatgtc gcggttgtat tttcgctggg taatgttatc tggtgattat
     7441 gggttcagtg acatatggaa aaagataagt gcgaacattg atactgaaga ataatatatc
     7501 caggaataga aaaacatgaa tttcggagat ggtttcttct gtctcctgtt tttcagggga
     7561 gtctgtgggt caaatgttat cagcgtcatt aaaggatacc ctttcataag aataaaaata
     7621 tagacgcata aatgagcgta aagaggtaac ctgtcctgcc agaacagaat catcatgatg
     7681 acggcaagat gaaatgtcgt tacattgata taataaaacc agctgtattt acgcagatac
     7741 tgttggatat tcacgctgtc agcatgcggt tctggtgaac aatttatgca aaaacagaag
     7801 aaagaaatga tgatgaacac ggtcagagtg accggtaata cagtcacaag cagaaagagt
     7861 atggacataa aaatatctcc gtcaggatag tgtgattatt cctttatggt tccataaccc
     7921 ggcgtagctc cgtcgactgg ttataatttt atccgggacc tggctttaag gggtaatgcc
     7981 acctctttcc attcactaca gcttccggga atacgttctg tgcgtcgtat tattcgtgtg
     8041 ttctggcata caataatcag ggcattgtct gccgtctgaa aaggcataat cgcaatgtta
     8101 atatcttctg ttgccgggat acagatgact ttcttttcac atgtattggt gtatatgaca
     8161 actctgaatt tttctgcgga cattttcaat cctcttttaa accagaatat cccgtgaggg
     8221 cattctttgt gaaaaagttc tgtggacttc tctatgacgg caggtaatgc atccagcagg
     8281 gcaagacgga agcggaggct ggtctgctca tccgaaagca cgttaatggc atcgttcagt
     8341 gcgcgctgtg cttctgcttc tgccagacct ttctgacggg tggcctccgc cagttcaata
     8401 attgctgctg cctggagaag tcagcggaga gtggctgccg ttgtggctgt cccggtattt
     8461 cagtgtaaca ggtgattgct gtgatctatg ccccattcgg ctttcggtaa acctgaatgc
     8521 cccacatgaa gaatgcgtga cagcatgttt ctgccgctgc cgttcaggct gcattcgcgt
     8581 ccgtgcgcga gtataaaacc ttcatcccgg aatatatctt ccacttctga agattcgagg
     8641 gatgccggat gaattaatga aaatgccacc ggtctgagca ttctgcccgt ggtggaaaag
     8701 gtgtctctgg tactgttcag ctgcaggata tgacgtttac ctgtttgcgc acgggaaagt
     8761 gtctcactca gcagctggtc gttccatgcg cacgtgccaa tctgccatcc cagacttctg
     8821 cacgcggaaa gagccgagcg gaagcggtac aggtcgtata atgctgcgtt attgccactt
     8881 tccagcagtg atttcagcgt atttgctacc ggtatgtccg tattagtggc cagcagtttt
     8941 cgcagagtcg taaactcttc tgccattcct ctgaactcgc gcatctgacg ggcagtgtca
     9001 agccagttca gcaggtaacg ccggcgacgc cgttcgttgt ttatgttgtc cggggcggtg
     9061 gagagtgctt cgagcatatt ctccagtaac agaatgacag ggttttcctg ggatagaatg
     9121 gtcataaagg cgtcctgaaa aaatgcccga cagccgctct ccgtacaccc tcaggtgtca
     9181 ccggtgccgg gcattgtgga aacagaaggg aaaaaaatcc cggccataaa ggccgggaaa
     9241 agtcgtcaaa ttaagacagc aattttcatg tcaacgctcc gtcatcagat accaggtgca
     9301 ggagcagggc gatcctcgac tccggcagag gctgtgcatc aagccggagg aggtactggt
     9361 aacgcgcttt tgtcagccag agggtggagg cagcatccag ctcctccctg gctgccggat
     9421 tgagcgcagg atccgggtac actttcatta aagactgaac gatatcctcc ctgacggttt
     9481 tgatttcacc ggccattttc acgaagccca gaaaggcact gtgacggcgc aggtgagtga
     9541 aagcgtggcg ttcggggtct gctttcagga aaaagtaacc gggaaacacg gcgtgtatat
     9601 gtcgacggta accacagact ttatccgtgc ggcggacttt tttcagtatc aggggtgtcc
     9661 agggaaggac gtgttgttcg gaaagccagg aaaagagatg ttcgcggttt ttgccggcag
     9721 ggatgtactg agccagatac cagttgcgtt gatgtaattc actgatattc aaaatccgga
     9781 acgctccgcg aaaatcgatc cccgcagggg ggcttcgatg ttgttgtgaa cggcgaatac
     9841 ataaagcatg tgtcggaaac agcggctgac ccacgttcag cccggcctgc agttctgaca
     9901 ggcacatgat ttacgcactc ttgtaagcct tgtggacggt ttcgaggcgg gcgaaaaccg
     9961 acaactcctg caaacaggat taatggctgg aaaatatatt acataaggat gtatctgtgg
    10021 caagagccaa gataagcagt tgaatagatc gttatatttg ataaataagc atattattct
    10081 gatttgtgtg cagtgtctgg aggattagat cgtttaaaaa ttttaggcga tctcttctac
    10141 taaaaatgat gaaaaataca gcataaaaga tcgtttgtga aattgttcat tttttgacac
    10201 agttcaaaaa aacgacgctt tttagggggt taaagagggc aactgtaacc ctgagaaatc
    10261 gtgtcggctt ttcaaagcgg tggaaaaggg gtatattgcg gatcgttatt cagtggtttt
    10321 tgggatcctc gcggtccgga aagccagaaa acggcagaat gcgccataag gcattcagga
    10381 tgtatggcag aaacgacggc agtttgccgg ggccggaagg ctgaaaaaag tttcagaaga
    10441 ccataaagga aaacccccat catctttcca cgaaacttgg cggaatcaga gaaagataat
    10501 gggggcctca cagaatacgg gataagtata tatgaaaccg tgtcagagat tcaacccttc
    10561 gcagtgcatt aacgcacggc aatctgttcc cgccataagc gacagcttgt ggcggctctg
    10621 aaccagacct catttactga aagcacatac cgtcaggtaa agaacccgaa tccggtgttc
    10681 acaccccgtg aagatgccgg aacgctgaag ttctgcgaaa aactgatgga aaaggccgtg
    10741 ggcttcacct cccgttttga ttttgccgcg catgtggcgt atgcccgttc gcgcggcctg
    10801 cgccggcgta tgccgccggt gctgcgccgc cgggcgattg atgcgctgct gcaggggctg
    10861 tgtttccact atgacccgct ggccaaccgc gtgcagtgct ccatcaccac gctggccatt
    10921 gagtgcggac tggcgacgga gtctgaggcc gggaaactct ccatcacccg tgccacccgt
    10981 gccctgaagt tcctgtctga gctggggctg ataacctacc agacggaata cgacccgaca
    11041 attggctgta acatcccgac cgacatcacg ttcacgccgg cactgtttga ctcactcgat
    11101 atctcagaag aggcagtggc ttccgcacgc cgcagccgcg tggaatggga aaacaggctg
    11161 cgcaaaaagc aggggctgga tgcgctgggt atggatgaac tgatagcgag ggcctggcgt
    11221 tttgtgcgcg agcgtttccg cagttatcag gcagagctta agtcccacgg catgaaacgt
    11281 gcccgtgcgc gccgcgatgc aggcaggacc cgtcaggaca ttgtcaccct ggtgaaacgg
    11341 cagctgacac gtgaaatcgc ggaagggcgt ttccgcggaa gcctggaggc cgtgaagcgc
    11401 gaaatcgacc gtcgcgtgaa ggaacgcatg ataatgtcgc ggaacaacaa ctacacacgg
    11461 ctggccactg cctcaccctg agtttgacct catctgaata atccggcctg cgccggaggc
    11521 ttccgcacgt ctgaagcccg gcgacgcaca aaaaagcagc acagcacaca aaaaacagcc
    11581 tcattatctg ccttccggct cgccttgctc cccctgtttt tcacgcaaaa caggtatcac
    11641 agacggggaa ttctgtttat ccacatttaa ctgcaaggga cttctccgta aggttacaac
    11701 cgttcatgct ataaagcgcc agccgcaggc gttacagggt gcaatgtttc ttttaaaccc
    11761 cagtactcta tttcctttaa actacttaat tacttccatt taaaaaaaag acctattcag
    11821 tgcctgtcct gtggacagac aaatatgcac ctcccacgct gacgcgcggg ccccgaacct
    11881 caccgttctg gaaccacaac aaaaaacatc agaaataaaa aaaccacaca aacgcagcac
    11941 cgtgcccacc cctcataact gaaaagcgag gccgcccccg cccgaagggc gggaacaaca
    12001 tcgcttttaa ttatgaatgt tgtaactaca ttatcatcgc tgtcagtctt ctggctggaa
    12061 gtcctcagta cacgctcgta agcggccctt acggcccgct aacgcggaga tacgccccga
    12121 cttcgggtaa accctcgtcg ggaccactcc gaccgcgcac agaagctctc tcatggctga
    12181 aagcgggtat ggtctggcag ggctggggat gggtaaggtg aaatctatca atcagtgccg
    12241 gcttacgccg ggcttcggcg gttttactct tgtgccatat gtagctacat tatgccgcct
    12301 tccatgccgc tcacgcggca tcagaacaat aaaaccagca ttaaatgtat ttgcaaaagt
    12361 atgagtaata cagacttgtt aaccggtcct ttcagaggta tctttaagac ttgttaaata
    12421 gtctcaaagg tacgttatgc ctaacatcat tctgtctgac accagtgcaa gtgtcagtga
    12481 actgaaaaaa aatcctatgg ctacagtcag tgccggagat ggttatcctg tcgccattct
    12541 taaccggaat cagccggcat tctactgtgt gcctgcggaa ctctatgaaa ggatgcttga
    12601 tgcgctggat gaccaggaac tggtgaaact ggttactgaa cgcagcaatc agccactgca
    12661 tgatgtggac ctggacagtt atttatgact tatacagtaa aattcaggga tgatgcgctc
    12721 aaagaatggc tgaaactgga taaatccatt caacagcagt ttgcgaaaaa actaaagaaa
    12781 tgcagcgaaa atcctcatat tccttccgca aaactgcgtg gattaaaaga ctgctacaaa
    12841 atcaaacttc gcgcatcggg gtttcgtctg gtctatcagg ttattgatga catgctaatt
    12901 attgcggttg tcgcggtagg taaacgtgaa cgtagtaacg tttataatct cgccagtgag
    12961 agaatgagat agttatcttt aaggctgact gatattactt caaaagtatt tctgatagtt
    13021 atgttatttt attccacaat gcagaatata ttctttattc tttaaggtcg tttttatgat
    13081 gaaaaatcca ttagcttatt ttcatattgc caccttttta ttgtcaggta aaagtcgtaa
    13141 tctgtcagaa tactggaaag ccgaagtcat catgcgggat aagtttgtct tacaccgcct
    13201 gataagaaac gggatgcaga gacaacgagg ctttctgatg tggtggcgtt tggccaatga
    13261 gatgtttatc tctggaaaca aaaaacaaag gaaatgtgcg ataaagataa aaaatgcact
    13321 tatggaacgg tatgggtgtg atatcggcct gggggcgcgg attggtaaag gtctcgtgtt
    13381 gcctcatcat gcaggtatcg tcattcatgg taatgttgat atcggtgaga atgtcattat
    13441 acggcagaat acaaccattg gtgaaaaggt ttctgacagc agggaaaact atattgttat
    13501 tggcgataat gtcgatattg gtgcccatac atgtattatt gggttaaatg tgaaaatcgg
    13561 aagtaatgtt aaaatcgggg caatgtcttt tattatggaa gatgtacctg ataactgtac
    13621 ttatgtcacc aggaaggaat ccagagtgat aattcgcgaa cagtaatgaa atacatgtta
    13681 tcattcttct gttggtgact ttgccgactc agatatatac tatctgtatg tcgtgcatta
    13741 tatattaacg atacgcagag gtgtgtatat gggcagtgtt acaaagaaac cgtcacctgt
    13801 tgttattatt aagaaaaaaa gacgaattct ttctcatcag gatgatgccg tacaggcagt
    13861 tcctgatgat ataaaaccag cgccagtaat ggaatttaaa accgactcat tacctcctga
    13921 aataccagcc acagtggccg gttccccgga acaacagaaa aaaacaggac gtaagaagaa
    13981 aaaaaagaag aacaatagat ggaatcatga gtttatccga cacagtctgg actgtgttaa
    14041 aaaaacattc cctcagttac gggctgaaga ggggggatat catgcattta aaataggcat
    14101 ctcaaaggat gtcatggctt ttatttcggc cagtcctgag tgtggactaa ctgtaaaaga
    14161 atggaattgt gctgtaagga tgattactgg cggttacaaa tatctgcagc gtatatcagt
    14221 cgcaggtact ccgcgttatg gtctggatgg caaagttcat ggaactgtaa cggaggaaga
    14281 agcgctgtat gcgcaggcaa aacttgaaaa acacacgcag cgttataacg ccaggatgaa
    14341 agagacagaa gatgctcgtt gcaaccaacc atcgaaaact gtcacaccgg acagcaattt
    14401 gaaggacgcc tgcattcata acggggcata attatgtgaa atgctgtccc gctatggaat
    14461 cagaattgag taacgacacg aggttttaat gattactgtt attatttctg aggtggatgg
    14521 ctggcgtgaa tggaaacacc gcgtccgaac aaaggatgca cagacagcca tcatccgggc
    14581 tatgaataaa cattttccac gatcttacaa ttttatccct gatgatattg ataatgcacc
    14641 tgtattattt gcagctgtaa cacgtacacc gaacgtgaaa ataaccggtc atatctggaa
    14701 acctatgtgg aacaggggaa tttgctggaa cgtcaaaggt ccgccagtaa tcatcacgct
    14761 catccagggg gccgcttgga attcggaaaa taaaccacgt taagactatt tggggttcta
    14821 agccgaaaac ctagaaaatt ccgtaaccaa agccagtaat tgacagattt gcatgacgtt
    14881 gaataggtga cgggttatgt gacgaaatct gatgcagaaa tcgttgtttc agtgacagtc
    14941 actcaatcgg tcgtttatgt gacaacccac gccgttactg gtcgcggaaa aatccaggtt
    15001 tcggggtttg aggctcagtg gaacaataac cgccgttaag cggtcatttt aaatagccaa
    15061 aaatcaacca gccaatttac atcgtgaatc tgcgttcttg gctatctgtt ttgggggggc
    15121 caagttgcct ttactcatcc tggccgtttg cggaacaaac gacgttgttg atctcaacag
    15181 attgatttac ctgttattga ccattcaatt gaaccgctta accgcctttt gagttccact
    15241 actccccaaa ccccaaatag ctgtctagcg cgaaaaatca ctgcccgtaa cgtatcatat
    15301 ggctggtacg aataaattct cacttactgc accataa
//
LOCUS       DBBGLQ010000105        15161 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.105, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000105 DBBGLQ010000000
VERSION     DBBGLQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.105"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            336..917
                     /gene="mdaB"
                     /locus_tag="ACIYSB_004217"
     CDS             336..917
                     /gene="mdaB"
                     /locus_tag="ACIYSB_004217"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGC5431135.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            948..1262
                     /locus_tag="ACIYSB_004218"
     CDS             948..1262
                     /locus_tag="ACIYSB_004218"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGC5431136.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1310..3202)
                     /gene="parE"
                     /locus_tag="ACIYSB_004219"
     CDS             complement(1310..3202)
                     /gene="parE"
                     /locus_tag="ACIYSB_004219"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGC5431137.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3231..3812)
                     /gene="yqiA"
                     /locus_tag="ACIYSB_004220"
     CDS             complement(3231..3812)
                     /gene="yqiA"
                     /locus_tag="ACIYSB_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGC5431138.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3812..4639)
                     /gene="cpdA"
                     /locus_tag="ACIYSB_004221"
     CDS             complement(3812..4639)
                     /gene="cpdA"
                     /locus_tag="ACIYSB_004221"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGC5431139.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4664..5086)
                     /locus_tag="ACIYSB_004222"
     CDS             complement(4664..5086)
                     /locus_tag="ACIYSB_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGC5431140.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5087..5716)
                     /gene="nudF"
                     /locus_tag="ACIYSB_004223"
     CDS             complement(5087..5716)
                     /gene="nudF"
                     /locus_tag="ACIYSB_004223"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGC5431141.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5921..7402
                     /gene="tolC"
                     /locus_tag="ACIYSB_004224"
     CDS             5921..7402
                     /gene="tolC"
                     /locus_tag="ACIYSB_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGC5431142.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7550..8221
                     /locus_tag="ACIYSB_004225"
     CDS             7550..8221
                     /locus_tag="ACIYSB_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGC5431143.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8227..9387
                     /locus_tag="ACIYSB_004226"
     CDS             8227..9387
                     /locus_tag="ACIYSB_004226"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGC5431144.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9425..10213)
                     /gene="ygiD"
                     /locus_tag="ACIYSB_004227"
     CDS             complement(9425..10213)
                     /gene="ygiD"
                     /locus_tag="ACIYSB_004227"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGC5431145.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVEGIEMGSLSMLSVQIG"
     gene            10356..11129
                     /gene="zupT"
                     /locus_tag="ACIYSB_004228"
     CDS             10356..11129
                     /gene="zupT"
                     /locus_tag="ACIYSB_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGC5431146.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11187..11357)
                     /gene="yqiD"
                     /locus_tag="ACIYSB_004229"
     CDS             complement(11187..11357)
                     /gene="yqiD"
                     /locus_tag="ACIYSB_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGC5431147.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11619..12272)
                     /gene="ribB"
                     /locus_tag="ACIYSB_004230"
     CDS             complement(11619..12272)
                     /gene="ribB"
                     /locus_tag="ACIYSB_004230"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGC5431148.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12375..12523)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12645..12935
                     /gene="ubiK"
                     /locus_tag="ACIYSB_004231"
     CDS             12645..12935
                     /gene="ubiK"
                     /locus_tag="ACIYSB_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGC5431149.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
     gene            13219..13770
                     /locus_tag="ACIYSB_004232"
     CDS             13219..13770
                     /locus_tag="ACIYSB_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGC5431150.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQSDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            13830..>15161
                     /locus_tag="ACIYSB_004233"
     CDS             13830..>15161
                     /locus_tag="ACIYSB_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGC5431151.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVIIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLSTLINNKRTPIHLDLQWVLIDNQTAVCVTPEQLTL
                     LGFTDEFIEKTQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWNHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQATNLDFPRIYLFRPIPAMNAKLTIGQYDTESSIFDSFHFSGISLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGRVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSY"
BASE COUNT         3968 a         3910 c         3578 g         3705 t
ORIGIN      
        1 attttctgct ttaacatata tcagtacgct cataaaaatc catttataag tatcaacatg
       61 acacgttgat actttcatct tttacttatt caaagccata ttttcaatcc ccaaaaaaac
      121 cgaaccaaac gtgtaaatta ttccggtgcg agtaagaatt atgaggaatg gctatcagta
      181 ttgtcatttt cagaaaatat ttatcctgca tcggtgagtc agagtaagat cagacttttg
      241 ctaaattcgc aaaagacttt gcacattttg ctaatttcac cgtaccgctc tgtgacgtac
      301 tatagtcggc aaacgtctca ccttgaggtt aaaaaatgag caacatcctg attatcaacg
      361 gcgcgaaaaa attcgcccac tccaatggtc aactgaacga caccctgacc gaagtcgcgg
      421 atggcacact gcgcgacctt gggcatgatg tccgcatcgt tcgcgccgac agcgactacg
      481 atgtcaaagc ggaagtacaa aactttctct gggctgatgt ggtgatctgg cagatgccag
      541 gctggtggat gggcgcgccg tggacagtga aaaaatacat tgatgatgta ttcaccgaag
      601 gtcacgggac gctgtatgcc agcgatggtc gtacccgcaa agatccgtcg aaaaaatacg
      661 gttccggcgg cctggtacag ggaaaaaaat atatgctttc tctgacctgg aacgcaccaa
      721 tggaagcctt caccgaaaaa gatcagttct tccacggcgt tggcgttgac ggtgtgtatc
      781 tgccgttcca taaagcaaac caattcctcg gtatggaacc gctgccgaca tttatcgcta
      841 atgacgtgat aaaaatgcct gatgttcccc gctatactga agaatatcgc aagcatcttg
      901 tggaaatttt tggttaacta gagctcaggc tttagaagga gttaaccatg cttaccgtaa
      961 tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca ggcggtattg gatcagtttg
     1021 ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca cggctatgcg ccaatggtgg
     1081 attgcgcagc tggcgtgagt ttccagtcta tggcaccgga ttctatcgtg atgattgagc
     1141 agtgggaaag catcgcgcat cttgaagcgc atctgcaaac cccgcacatg aaggcgtata
     1201 gcgaagccgt aaaaggtgac gtgctggaga tgaatatccg tattctgcag ccagggattt
     1261 aatcctgcct tgtttgcccg gccatcctga ccgggcaatg ttctttcctt taaacctcaa
     1321 tctccgccat gtcgcctttc tcttgcaacc agttgcggcg atcttccgag cgtttcttcg
     1381 ccagcagcat atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg atagtcaact
     1441 gcaccagacg gcgagtgttc ggatcaagcg tggtttcgcg caattgcatc gggttcattt
     1501 cccccagacc tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt aattgctcaa
     1561 gtacgccctc tttctcttct tccgtcagcg cgtaataaac ctctttcccg agatcaatac
     1621 ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg cggaaatgtt
     1681 ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc atcagagtcc gcatccgcga
     1741 ggatacagat tttgccataa cgaagctggc tcagatcgtc gctgtcagga tcgataccga
     1801 tcgctaccga aatatcgtgc acttcctgcg aagccagcac ttcgtcggaa gagacttccc
     1861 aggtgttaag gatcttacct ttcagtggca tgatcgcctg atattcgcga tcgcgcgcct
     1921 gcttggcaga tccgcctgcg gagtcacctt ccacaaggaa cagctcggta cggttaaggt
     1981 cctgcgcggt acaatcagcc agtttgccag gcaacgccgg gccgctggtc agctttttac
     2041 gcaccacttt tttggccgca cgcatacggc gctgggcgct ggaaatcgcc atctccgcca
     2101 gcagttcagc cgcctgaacg ttctggttca gccacaggat aaaggcatct ttcaccacgc
     2161 cagaaacgaa tgccgcgcat tgacgcgaag agagacgctc tttcgtctgc ccggcaaact
     2221 gcggatcctg catttttact gacagcacat aggcgcagcg atcccagata tcttccgccg
     2281 acagctttac accgcgcggc agaatattgc ggtattcaca gaactcacgc atcgcgtcca
     2341 acaggccctg acgcagacca ttaacatggg taccgccctg catcgttggg ataaggttga
     2401 cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc cagtccacag
     2461 cttcagtatc accagcgaaa ttaccgataa acggtttttc cggcagcgtc ggcagaccat
     2521 ttaccgcttc cgccaggtaa tcattcagac cgtcctgata gcaccagcgt tgttcggtat
     2581 tgttgatctc atctttaaaa gtgatctcaa cgccagggca caataccgct ttggctttca
     2641 gcacatgcgt caggcgtgaa acagaaaatc gcgggctgtc aaagaaggtt tcatccggcc
     2701 agaagtgcac actggtacca gtattgcgtt taccgcaagt gccgacaacc tgtaaatcct
     2761 gcaccttttc gccattttca aaggcgatgt tataaacctg accatcgcgg cgcacgttaa
     2821 cttctacgcg cttcgacagg gcgttaacca ccgaaatccc cacgccatgc aggccgccag
     2881 agaactggta atttttgtta gagaatttac cgcctgcatg cagacggcaa agaatcagtt
     2941 caaccgccgg tacaccctct tccgggtgaa tatccaccgg catcccgcgc ccatcgtcaa
     3001 taacttctaa cgactggtca gcatgtaaaa taacgtccac gcgttttgcg tgacccgcca
     3061 gtgcttcatc cacactgtta tcaatgactt cttgccccaa atggttaggg cgagtggtat
     3121 cggtatacat ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc tcaatggcat
     3181 cagcgttata agtttgcgtc atggtttaag ttagtaattc gagttgatcg tcagagatgg
     3241 tgcagaccaa gaaaatcgac gatcgggttg aaataatctt cgaagcccgt gaatgcgtgg
     3301 ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt acgccaccgc ctggcggtaa
     3361 tccagcactt catctcccgt ctgttgcagc agccagatca aatccggcgc ttccagcggg
     3421 tcaatctgca tgactttaag atcgtaaata tggcgtgact ctagcacata ttgctgcccg
     3481 gtgtaggggt tctcgttctg accgagatag tccgtcagca gttcaaacgg gcgcaccgcc
     3541 gggtttacca ccactgcggg cagcataaaa cattgtgaca accaggtggc gtaatatccc
     3601 cccagtgacg aaccgacaat acccagcgaa tcaccgccat gttccaggac aatggattcc
     3661 agcagctctg ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat ctcaacgtca
     3721 gggtgatgtt ccgccagcca gttttttaac aagctcgctt ttgcagagcg cggcgagctg
     3781 ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc ttctgaagcg gtatcaggtt
     3841 ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag cgtgccatca gcatgtaact
     3901 cgagagtacg ccagccgggc gcgatggtat ccagcgtaaa gttggaacag tgcggcttaa
     3961 actgcacaca ggtcgacggc gttgccagca ggcggcgacc attccagtcg agatccagct
     4021 cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc agcacggtat
     4081 ccagttcgcc cgcgttacgc agactgtgtt gatcgagcca actacaaccc gcaggtagcg
     4141 gatgatgatg cagcagcagc aacgtatggc gttctggcgc atcggccagt ttacgttcca
     4201 gccactcaag ctgaaactcg ctcagctcac cgtgcggcac gccaaacacc tggctatcca
     4261 gcaacaggat ttgccattgc tcaccaataa acacgcgctt cgccggggag atacccgcat
     4321 cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt gcccggcagc cagacgcagg
     4381 gcgcacgaaa acttgcgatg ccttcagcga aatgctgata ggccgcagag gattgatcct
     4441 gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg ctggtgtggc cgaatcgcct
     4501 ccagcaccgc ctggtaactc tcccaggtgt ttacccctaa cagggcttcg tgcttttgtg
     4561 caaacaggtg agtgtcggta atttgtaaaa tcctgactct ggcctcacca gccagaggaa
     4621 gggttaacag gctttccaaa tggtgtcctt aggtttcacg acgctaataa accggaatcg
     4681 ccatcgctcc atgtgctaaa cagtatcgca accagtccgc taaaaactga ttaatttgat
     4741 gcttttcgtc gcgttgatgc aactttttat taggataatc ataccgcgct ttgaagcgaa
     4801 aaatctgctg gcttgaacac acttcagcca ccatcgcgtc atgatacaga cgcaccgtca
     4861 ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc accagggtag
     4921 tgtatcgggt cgattccaca atcgtcagcc gatattgtgc gtttgccacc tgatagctta
     4981 cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa aagttcatct
     5041 cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg cttcatttat gcccactcat
     5101 tttttaacgc ttgatgatgc agctgcagcc attgcaaagc gatgaccgac gctgcgttgt
     5161 cgattttccc ctcttctacc cactggtatg cctgttcccg gcttaccaca tgaacgcgaa
     5221 tatcttcgtt ttcatcagcc agaccgtgaa taccgcttgc ggtcgtggcg tccacttcgc
     5281 ccaccataat tgacgaacgc tcactggtgc cccccgggct tgccaggaaa cttaacaccg
     5341 gtttggtccg tttgactatc agtcccgcct cttcaatcgc ttcgcgacgg gcaacatctt
     5401 ccacactttc accctcttca atcatcccgg caaccatctc cagtagccaa ggggtttcgc
     5461 tggtgtcgta cgcggcaatc cgaatctgct caatcagcac aacttcatca cgcactgggt
     5521 caaagggtag caagactgcg gcgtgaccgc gctcaaaaat ttcccgccgc acctcatgac
     5581 tcatttgccc gttgaataga cgatgacgaa atctataaag atctaatgaa aaaaagccgc
     5641 gataaagtgt ttctcgtgca ataatttcta catcgttttt gccaaatgta acgggcaggt
     5701 tgtctggctt aagcattgtt aatgtcctgg cactaatagt gaattaaatg tgaatttcag
     5761 cgacgtttga ctgccgtttg agcagtcatg tgttaaattg aggcacatta acgccctatg
     5821 gcacgtaacg ccaacctttt gcggtagcgg cttctgctag aatccgcaat aattttacag
     5881 tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa atgaagaaat tgctccccat
     5941 tcttatcggc ctgagccttt ctgggttcag ttcgttgagc caggccgaga acctgatgca
     6001 agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg ccgatcgtga
     6061 tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc taggtttagg
     6121 tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact ctaacgcgac
     6181 cagtgcgtcc ttgcagttaa ctcaatccat ttttgatatg tcgaaatggc gtgcgttaac
     6241 gctgcaggaa aaagcagcag ggattcagga cgtcacgtat cagaccgatc agcaaacctt
     6301 gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg ttctttccta
     6361 tacacaggca caaaaagaag cgatctaccg tcaattagat caaaccaccc aacgttttaa
     6421 cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc gcacagtacg ataccgtgct
     6481 ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc tgcgccagat
     6541 caccggtaac tactatccgg aactggctgc gctgaatgtc gaaaacttta aaaccgacaa
     6601 accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt cgctgttaca
     6661 ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc caggcgcagg atggtcactt
     6721 accgactctg gatttaacgg cttctaccgg gatttctgac acctcttata gcggttcgaa
     6781 aacccgtggt gccgctggta cccagtatga cgatagcaat atgggccaga acaaagttgg
     6841 cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg tgaaacaggc
     6901 acagtacaac tttgtcggtg ccagcgagca actggaaagt gcccatcgta gcgtcgtgca
     6961 gaccgtgcgt tcctccttca acaacattaa tgcatctatc agtagcatta acgcctacaa
     7021 acaagccgta gtttccgctc aaagctcatt agacgcgatg gaagcgggct actcggtcgg
     7081 tacgcgtacc attgttgatg tgttggatgc gaccaccacg ttgtacaacg ccaagcaaga
     7141 gctggcgaat gcgcgttata actacctgat taatcagctg aatattaagt cagctctggg
     7201 tacgttgaac gagcaggatc tgctggcact gaacaatgcg ctgagcaaac cggtttccac
     7261 taatccggaa aacgttgcac cgcaaacgcc ggaacagaat gctattgctg atggttatgc
     7321 gcctgatagc ccggcaccag tcgttcagca aacatccgca cgcactacca ccagtaacgg
     7381 tcataaccct ttccgtaact gatgacgacg acggggcttc ggccccgtct gaacgtaagg
     7441 caacgtaaag atacgggtta tctgccgcat tcttccccct tctcgcttca atttcgacca
     7501 gccatcctct attctgatgg gtatttacca ctggtcccgg aagacaaaaa tgaaacggac
     7561 aaaatccata cgccacgcat cgttccgcaa aaactggagc gcacgccatc tgacaccagt
     7621 cgctctcgcg gttgccactg tttttatgct ggctggctgt gaaaagagtg atgaaacagt
     7681 gtctctctat caaaatgctg acgactgttc agctgcaaac ccaggcaaaa gcgccgaatg
     7741 taccaccgcg tacaacaatg cgctgaaaga agccgaacgt actgcgccga aatacgccac
     7801 ccgtgaagac tgtgttgctg aatttggtga aggtcagtgc cagcaggcac cagcccaggc
     7861 tggcatggca ccagaaaacc aggcgcaggc ccagcaatcc agcgggagtt tctggatgcc
     7921 gctgatggcc ggttacatga tggggcgtct gatgggcggc ggcgcgggat ttgcacagca
     7981 gccgctgttc tcctcgaaaa acccagccag tccggcttac ggtaaatata ccgacgcgac
     8041 gggtaaaaac tatggcgcag cccagccagg ccgcaccatg accgtaccga agacggcaat
     8101 ggcaccaaaa ccggcgacca ccactaccgt tacccgtggc ggttttggtg aatctgttgc
     8161 caaacaaagc actatgcagc gtagtgcaac cggtacctct tctcgttcaa tgggtggctg
     8221 ataccgatgg aaagagtcag tattaccgag cgcccggact ggcgtgagaa agcccacgaa
     8281 tacggtttca attttcacac catgtacggc gagccgtact ggtgtgaaga tgcttactac
     8341 aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact gcaccagatg
     8401 tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc tgatgaccaa attccgcatt
     8461 ccaaaacaca cctggagttt tgtgcgccag tcatggctga cgcaccagcc atcgctttat
     8521 tcgcgtcttg atctggcgtg ggatggcact ggtgaaccta aacttctgga aaataacgcc
     8581 gatacgccaa cgtcactata cgaggcggcg ttctttcagt ggatctggct ggaagatcag
     8641 cttaacgccg gtaacttgcc ggagggcagc gaccagttta acagtctgca agaaaaactg
     8701 atcgatcgct tcgttgagct gcgtgaacag tatggcttcc agttgctgca tctcacctgc
     8761 tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt tgcaggactg cgcaacggaa
     8821 gctgaaattg ctactgagtt cctctacatc gatgatatcg ggttaggtga aaaaggtcag
     8881 ttcacggatt tacaggatca ggtaatttcc aacctgttca aactgtatcc gtgggaattt
     8941 atgttgcgtg agatgttctc aaccaagctg gaggatgcag gcgtacgctg gctggaaccg
     9001 gcgtggaaga gcattatctc caacaaggca cttctaccgc tactgtggga gatgttcccg
     9061 aatcacccga acctgctgcc cgcttatttt gcggaagatg atcatccgca aatggaaaaa
     9121 tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat tgagaacggc
     9181 aaaaccattg aagcagcgga aggtccgtat ggcgaagaag ggatgattgt tcagcaattc
     9241 cacccgttac cgaaattcgg cgacagctat atgctgattg gtagctggct ggtgaacgat
     9301 caacccgccg gaattggcat tcgtgaagac cgtgcattga tcacccagga tatgtctcgg
     9361 ttttatccac atatttttgt tgaataagcc acgataccgg atggcactcg ccatccggta
     9421 attgttagcc tatctgcacc gacagcatac tcaggctgcc catttctata ccctcaaccg
     9481 gaatggtaat tggctcctgc ccatcccacg cacctaacac atacaacaac ggcaaataat
     9541 gctctggcgt tgggttcgat aacgtgccac cttcatggtc gaggtaattc accagaggat
     9601 gttgttccac tggcccttgc cacgtcagat tcgctttcac atactcatta aacgacgtcg
     9661 cccacggata cggtgaacta tcaccgtgcc acttcactgt gcgcaggtta tgcaccacgt
     9721 taccgctggc gaccaacatt attccttcat ctcgcagcgc tgccagtttg cgccccattt
     9781 cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa ctgcaccatc gggatatcag
     9841 cgtcaggata catcttaatc agcacgcccc acgagccgtg gtcaaagccc caggcttctt
     9901 tatccagcgt caccgggatc ggcgctaaca gctcaaccag acgctgtgcc agcgcaggcg
     9961 aacccggagc aggataatgc gtatcgtaca gcgcctgcgg gaagccacca aagtcatgaa
    10021 tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg ggtaaaccag tgagccgaaa
    10081 ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa cttctgccag ctgcgggtat
    10141 acaaattatc ttccagcacg ttcatcggac taccgtgacc taaaaacaat gctggcatac
    10201 gtgttgaaga catgatgata tccttaacta aaggtgtcat tttgatatcc tcacaatacg
    10261 cttgttcggc ggagtaagaa cccggataac aatgatgatg atcatcagtt attttgacga
    10321 tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc agtacctctc attctgacca
    10381 tactggcggg ggcagccacg tttattggcg cgtttctcgg cgttctcggg caaaaaccct
    10441 cgaaccgctt actggcgttt tcgctaggtt ttgcggcggg gatcatgttg ctcatctcat
    10501 taatggaaat gcttcctgcc gcactagcag ctgaaggaat gtcgcctgtg ttgggttatg
    10561 gaatgtttat cttcggtctg cttggctatt ttggcctgga ccgcatgttg ccacatgctc
    10621 atccgcagga tttaatgcaa aaatcggtgc agccgttgcc aaaatcgatc aagcgcacag
    10681 ccattctgct cactctcggc atcagtctgc ataacttccc ggaagggatt gccacctttg
    10741 tcacggcgag cagcaacctg gagctgggat ttggcatcgc actggccgtc gcgttgcaca
    10801 atatccctga aggtctggca gtggcaggcc cggtttatgc ggcaacgggg tctaaacgca
    10861 ccgcgattct gtgggcgggg atttctggac tagcagaaat tcttggtggt gtgctggcgt
    10921 ggttaatcct cggtagcatg atttccccgg tggtcatggc ggcaatcatg gcggcggttg
    10981 caggaattat ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa gagattgacc
    11041 ctaataataa ccccagctat ggcgtactat gtggaatgtc agtgatggga ttcagtttag
    11101 tgctgctaca aacggcggga attggttaaa aacaacatca gaagttgtcc agcgcatgga
    11161 caacttctgc cagcttcacg aaagccttat tctacctttt cggctgtctc ttctctcgta
    11221 ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc tgtcctggcg aaacgcccga
    11281 caataacgtt tcaaatgcag gaaataaccc accacaacca gagcaatcaa tacaatccag
    11341 taccaggcga taaacatcct tttaccctta atatttaaca atactaaaca tttagcgtat
    11401 aaatttcaca tatccttttt cggatatatt catatggtcg cataataacg gaacaattat
    11461 atgattttga tcataatgaa aaagaatgcc tgatattaat gaaattttct catagaaaga
    11521 gaagcggaat taacatcttg catagcaggg aaaaataaaa accgaagcct gctacaactt
    11581 cggtttcttg attaaggcag taaattaagc agcggttttc agctggcttt acgctcatgt
    11641 gcctgacggt atgccaccag gtcttcaata gtcacgagcg ccatattgtg tttattggca
    11701 aactcaatac actctggtgc acgcgccatc gtgccatcgt cattagtcag ctcacacagt
    11761 acaccagccg gtttaaagcc tgccagcgtc atcagatcaa tagttgcttc agtatgaccg
    11821 ccacgcgtca gtacaccacc tgcctgagcg cgaagtggga aaacgtggcc aggacgattc
    11881 agatctgacg gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat acggtcagcg
    11941 gcagaaacac cggtagtcac accttcagct gcttcaatgg tcacggtaaa accggtgcca
    12001 taggcgctgg tgttattttc taccatcatt ggcagatcga gttgtttacg gcgatcttca
    12061 gtaatgcaca ggcaaacaat accgctaccg tggcgaatgg tcagcgccat ctgctcaaca
    12121 gtcatggttt ctgccgggaa gatcatatca ccttcgtttt cacggtcttc atcatcaagc
    12181 accattacac cgcgtccttc acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc
    12241 gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa acctcactaa aattatggtt
    12301 accagaatca gggcagtctt aggagtggcg gcatatagcc aaaataacgt gagcgggtcc
    12361 atgcccgaca gaatcgttac tctctcccat ccggactcta accgtcggcc ccggaattac
    12421 accggatctg ctgtcctttg agttcgcacc caaagcgctc gcgggctttc aactgagttg
    12481 atttaccgcc ggtggggaat ttcgccccgc cctgagaata agcggattca ctataacgct
    12541 aatgattagc ggcagcaacg catagcttca cataattctg gtttatgact tacccttatc
    12601 gcactacaat ggcactcaac acctatcagt acagggaaac tacaatgatt gacccgaaaa
    12661 aaattgagca aatcgctcgc caggttcacg aatcaatgcc taaaggaatc agggagttcg
    12721 gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc gcagctgacg cgcctcgacc
    12781 ttgtaagccg tgaagagttc gacgtccaaa cgcaagtcct gttacggact cgtgaaaaac
    12841 tggcgctgct tgaacaaaga atcagcgaac tggaaaatcg cagtactgag atcaaaaagc
    12901 agccagatcc agagactctc cccccaacgc tgtaaacatt cgtctttaat gcactgtaaa
    12961 ttatgaatta atcctctaat ttatagtgct ttctattacc ttatcgcgta atcccattca
    13021 gaaaaccatc acaaaaatga atgagtacaa ttgaaagtat aattgcaatg tattttttaa
    13081 gttttactta atcgatattt agataaacta attttataaa aaacacaaaa taaaatatca
    13141 tatttcaatg attagtagtc cttaaataac agttactaat cattctcttt tctacttatc
    13201 tttatataaa ggaatattat gtccgctttt aaaaagtcac ttcttgttgc tggcgtagca
    13261 atgattcttt ccaataacgt gtttgctgat gaaggtcacg gtattgttaa atttaaaggt
    13321 gaagttatct ctgctccatg ttctattaaa ccaggtgacg aagatttaac tgtaaatctg
    13381 ggtgaagtag cagataccgt actgaaaagc gatcagaaat ctttggctga acctttcact
    13441 attcacctgc aggactgcat gctgagtcag ggtggaacta cttattcaaa agcgaaggtt
    13501 actttcacta cagcaaatac catgacgggt cagtctgatc ttctgaaaaa taccaaagaa
    13561 actgaaatcg gcggcgccac tggcgttggc gtacgtattc tggacagcca gagcggcgaa
    13621 gtaactttag gtactccggt agtaattacg ttcaacaata ccaacagcta tcaggaactg
    13681 aatttcaaag ctcgtatgga atccccaagc aaagatgcca ccccgggcaa cgtttacgct
    13741 caggctgact acaagattgc ttacgagtaa tcgcatttgg ccgggttcgc ccggccacat
    13801 cattaacgga ttaatgataa gtggatcaga tgtataaaaa attaaaatta accacaataa
    13861 gcgaattgat taaaaatatt tattgttcat tatccgttat catcattggt tgtgcgtcag
    13921 cttatgccgt tgaattcaac aaagatttaa tcgaagccga agatcgtgaa aacgttaacc
    13981 tttcccaatt tgaaactgat ggccaattac ccgtcggcaa atattcacta agcactctga
    14041 ttaataataa gaggacgcca atccaccttg acctccaatg ggtattaatt gataaccaaa
    14101 ctgcagtttg cgtgacacca gagcaattaa cattattagg atttactgat gaatttattg
    14161 aaaaaactca gcaaaacctg atcgatggtt gttaccctat cgaaaaagaa aaacaaatta
    14221 caacttatct cgataaaggg aaaatgcaat tatccatatc tgcacctcag gcatggttaa
    14281 aatacaaaga tgcaaactgg acgcctcctg aactttggaa tcatggtatt gctggggcat
    14341 ttcttgacta caatttatat gcctctcatt atgcaccaca tcagggcgat aattcgcaaa
    14401 atataagttc ctatgggcag gctggggtta atcttggggc ctggcgcctg cgtactgatt
    14461 accagtacga tcagtcattt aacaatggca aaagccaggc gaccaacctg gattttccgc
    14521 gtatttattt gtttcgccca atcccagcaa tgaatgcaaa actaactata ggtcaatacg
    14581 atactgaatc ctctattttc gactctttcc atttttctgg catttcgttg aaaagcgatg
    14641 agaatatgtt accgccagac ctacgtggtt acgcaccgca aatcacgggt gtcgcacaaa
    14701 cgaatgcaaa ggtcactgtc tcacagaaca accgtattat ttatcaagaa aatgttcctc
    14761 caggcccatt tgctattacc aatttattca atacattaca ggggcaactt gacgtcaagg
    14821 ttgaagaaga ggacggacgc gttacgcaat ggcaagttgc atctaatagt attccttatc
    14881 tgacgcgtaa agggcagatt cgctacacca ctgctatggg taaaccgacc agcgttggtg
    14941 gtgattcctt acaacaaccc ttcttctgga ctggtgaatt ctcatggggt tggctgaaca
    15001 atgtatccct gtatggtggt tcagttttaa caaaccgtga ttatcaatct ctggctgccg
    15061 gcgttggttt taaccttaac tcattaggtt cattatcttt tgatgtcaca cgatctgatg
    15121 ctcagttgca taatcaggat aaagaaacgg gttatagcta c
//
LOCUS       DBBGLQ010000106        15053 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.106, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000106 DBBGLQ010000000
VERSION     DBBGLQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.106"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..199)
                     /locus_tag="ACIYSB_004234"
     CDS             complement(<1..199)
                     /locus_tag="ACIYSB_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGC5431152.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQWIDQVSPSQWAAIGVLGS
                     LALGFLTYLTNLYFKIKEDRRK"
     gene            complement(267..1319)
                     /locus_tag="ACIYSB_004235"
     CDS             complement(267..1319)
                     /locus_tag="ACIYSB_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGC5431153.1"
                     /translation="MANTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKHE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERVLFAEHYQGPYRGKSDGYAAKERELKQHIMA
                     PLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQPHHQLVASYDSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWMHKPV
                     QFYTGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELES
                     ERFNQTVKEINELVGK"
     gene            complement(1470..1667)
                     /locus_tag="ACIYSB_004236"
     CDS             complement(1470..1667)
                     /locus_tag="ACIYSB_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGC5431154.1"
                     /translation="MLKQQDMTETARVVLNELSVTEPATVGEIAQNTYLSRECCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(1894..2715)
                     /locus_tag="ACIYSB_004237"
     CDS             complement(1894..2715)
                     /locus_tag="ACIYSB_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGC5431155.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASVSTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRTRATELLADYALSRCDRVAALRKLPAEIKPTVMRIMASY
                     AFEDYARSAASKKQCPCCGGKKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACNDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEESWAERQLKRVTR"
     gene            complement(2712..3086)
                     /locus_tag="ACIYSB_004238"
     CDS             complement(2712..3086)
                     /locus_tag="ACIYSB_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGC5431156.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGECYRRDVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIMHDGEVQK"
     gene            complement(3086..3343)
                     /locus_tag="ACIYSB_004239"
     CDS             complement(3086..3343)
                     /locus_tag="ACIYSB_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431157.1"
                     /translation="MNNQAITFTPEQLRKQAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRTQKAVDELVDCVADLETKVGKFEKLVQEVLR"
     gene            complement(3340..4740)
                     /locus_tag="ACIYSB_004240"
     CDS             complement(3340..4740)
                     /locus_tag="ACIYSB_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGC5431158.1"
                     /translation="MTKPTNPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSMEKGAIQPVHIDDVL
                     PEVVERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTKHHLTKHPETALVVVDYLGLIKTRTTGRHDLAVGE
                     ISKGLKGLAKSGGFPLIALSQLSRSVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPDTQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPVKPGSPR
                     RYSNSRISGSKTERLF"
     gene            complement(4737..5627)
                     /locus_tag="ACIYSB_004241"
     CDS             complement(4737..5627)
                     /locus_tag="ACIYSB_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGC5431159.1"
                     /translation="MTNFLNKQHMQYDKAQLLNRRADLEEELAFVRGGKRSWRCEHWV
                     QSSETVSCGKHGKYTRYVLTGPNVRGKAVKRVSGCLSCLSEELDSVYDELRALKVREL
                     LDQAGIARRFQDCEFENYQAVNTDAQKNLEACQRYAASWKKCLAAGTSMVMIGNCGTG
                     KNHLAVSMAKNIIRHHQATIEITDVMRLTRAVKNTWRHNSERTDDEVINHFASLDLLI
                     IDEVGVQFGTPTEITILQEIINARYESVLPTILISNLTFEQLKESIGERIVDRVTDGG
                     CNCLVFGWESYRAHIRGVAA"
     gene            complement(5645..6628)
                     /locus_tag="ACIYSB_004242"
     CDS             complement(5645..6628)
                     /locus_tag="ACIYSB_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGC5431160.1"
                     /translation="MSRYAPTPEVMAIGQINISGNVTPANWWKHIRLPSGRPDATAIA
                     LLSEIVYWYRPTEVRDEHTGALLGYRKRFQGDKLQRSYQAFAEQFGFGKRETADALKR
                     LRDAGFITLDLRTVEMLDGVKCSNILFVGINPQAVAAITTPSSVSSESNSNNTVSDTA
                     TAITLKRNTPQRHNGTGDTPNVDTNTEITTEITTETKNTIGASADASAPARSARQEYS
                     PEFEQAWQEYPKRAGGNSKSAAFKAWKARIREGIKPETMLDGVKGYAAWVRATGNTGT
                     QFVKQAATFFGPDRHFEDFWQQPAAPGGGRGKSLPISGFSEQDYGSTNFNW"
     gene            complement(6625..6924)
                     /locus_tag="ACIYSB_004243"
     CDS             complement(6625..6924)
                     /locus_tag="ACIYSB_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5431161.1"
                     /translation="MNCFQFVCGSVFDNPIQRLIMLRVLMSGSSDGEGERVIDHQVLA
                     DFCCCSKQAMFRETLALERAGYLRIRKIATLTIDAKARLQPARGYTIVMPRKEAV"
     gene            complement(6921..7145)
                     /locus_tag="ACIYSB_004244"
     CDS             complement(6921..7145)
                     /locus_tag="ACIYSB_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431162.1"
                     /translation="MIRRIVNFLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQKVV
                     CQVHGRTHTLSYPLVAFQSGKMFKRLGGAV"
     gene            complement(7142..7375)
                     /locus_tag="ACIYSB_004245"
     CDS             complement(7142..7375)
                     /locus_tag="ACIYSB_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431163.1"
                     /translation="MCNNHYSETAATVIKTLMDALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQISSDRALETASKMIMADVQEVHP"
     gene            complement(7368..8033)
                     /locus_tag="ACIYSB_004246"
     CDS             complement(7368..8033)
                     /locus_tag="ACIYSB_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HGC5431164.1"
                     /translation="MNVSSLNIDSAISGRYTVPAPYKAGAGGRSPELIKAIYDAPASF
                     LSSALMHARIMVGWAGEPKGSPVSLIAGTANPVQFTTHEFRSSGGGSFSPIKEAAIMA
                     TVPTSPFLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCLEAFHEAENSLNNSTFRRNQREA
                     RHV"
     gene            complement(8146..8853)
                     /locus_tag="ACIYSB_004247"
     CDS             complement(8146..8853)
                     /locus_tag="ACIYSB_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGC5431165.1"
                     /translation="MNTAIFNDKASMTSIEIAELVGSRPDSVKRTIETLAKKGIIQFP
                     QTVEIENKQSLGPRRFSSAYVFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVSHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            complement(8850..9113)
                     /locus_tag="ACIYSB_004248"
     CDS             complement(8850..9113)
                     /locus_tag="ACIYSB_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5431166.1"
                     /translation="MKHKAIEKAIEILGSQQALAKRCGKAQSTVCDWLNCKNKISPEF
                     VPLLVAAVDGKIQAHEFRPDLPELFPHPSLAHTEDDKPTGDQQ"
     gene            9233..9925
                     /locus_tag="ACIYSB_004249"
     CDS             9233..9925
                     /locus_tag="ACIYSB_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5431167.1"
                     /translation="MTFSERLDLAMRNAKFTQGRLAKEVGMAQSSVNQLLNKANGSRK
                     TVEIAKVLGVNPEWLASGVGPMEIVTSADSHQIRNISEDWVTDSYVVDVLDIRYSCGP
                     GSYNSDYPDIVRSIAIEPGYASRIFGGRPASAIKAINAHGDSMKGTINPEDLVFVDIS
                     VRSFNGDGIYAFTYSGTSHIKRLQKVKDTLTVISDNPAYKDWAIEQADFEQLHIDGKV
                     IVSWPMTLHRFA"
     gene            10073..10723
                     /locus_tag="ACIYSB_004250"
     CDS             10073..10723
                     /locus_tag="ACIYSB_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5431168.1"
                     /translation="MSTYMGLPTNQHDALENIQLLVGAGALLYSSNNPELIELASSIL
                     DVTQKYSLNAARSRKQNLAINSQNRIITQREACGLTTAELARLLDLDEEIIIQWESGE
                     YEPTISMLIPLANVLGCDPLSLLSENNTAVPVRVNVPEVNAESIGARIESVRKKLGLT
                     ESDLARMIHTYSDPINDWECGICEVPADQIVPLASALHCDPMWLLTGKPTDGGTCA"
     gene            10720..11085
                     /locus_tag="ACIYSB_004251"
     CDS             10720..11085
                     /locus_tag="ACIYSB_004251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431169.1"
                     /translation="MKKFENITVLYVDEFDYANQEFLPEVVKAIDVADIVIRGKRIVK
                     NRLACTSRSNDRKTSQQDNYEGICLEPDSFAVNIYHLLHATQVLHMSSNYETKTLGSE
                     ILNFACEYAKAAAEKELAQ"
     gene            11283..11471
                     /locus_tag="ACIYSB_004252"
     CDS             11283..11471
                     /locus_tag="ACIYSB_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431170.1"
                     /translation="MEEKEQNITHEKVRVLLTIENGKVIHSKHLLDNEFVGCIDTFLW
                     MAKRAGYTIIPPAKEQTL"
     gene            11468..12049
                     /locus_tag="ACIYSB_004253"
     CDS             11468..12049
                     /locus_tag="ACIYSB_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431171.1"
                     /translation="MNHSEFRPEVTPHGIKIGNTTIDYAEAVQRLNDGEYDYPCWHGL
                     RIMQCIAEADDAGLLGRFSVDLKVAQWRWLYVATFISEEEDKNGTIDIPNDNGTTDRA
                     VIYKGKHGCLSIYPGPLRIALQNHVEWGFIEKYGEAEGMGRVLFLYQKMLIADPDNGF
                     IVSAMGREGLELLLDEMTHDLNTHGIPEAPVTH"
     gene            12059..12337
                     /locus_tag="ACIYSB_004254"
     CDS             12059..12337
                     /locus_tag="ACIYSB_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431172.1"
                     /translation="MANQNKINVFQVESKHKTPVIKHVRRHTRIYTPEEFIAMPMIKK
                     FIRDNPDHIAIDKNNGEIMLSRELAEIYYNVNNSKKLKKAIRKISGGA"
     gene            12334..12522
                     /locus_tag="ACIYSB_004255"
     CDS             12334..12522
                     /locus_tag="ACIYSB_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431173.1"
                     /translation="MNIYFRIVISLAIIACIYGLLVPFLISMKDTVAVISGFALAFLT
                     PPCIYAIYKGLSFTKDKR"
     gene            12519..13346
                     /locus_tag="ACIYSB_004256"
     CDS             12519..13346
                     /locus_tag="ACIYSB_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGC5431174.1"
                     /translation="MKKIIFSLAIVLPAIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDDPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQAEMTP
                     IGIQVMSLSYVGKPEYPSTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTNGASTPFIQVK"
     gene            13386..13745
                     /locus_tag="ACIYSB_004257"
     CDS             13386..13745
                     /locus_tag="ACIYSB_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HGC5431175.1"
                     /translation="MNTHNTQPKIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERDIIATINIGGLECKDEDLQGELWLAEM
                     LVSAEIISIEPDTGIQL"
     gene            13776..14018
                     /locus_tag="ACIYSB_004258"
     CDS             13776..14018
                     /locus_tag="ACIYSB_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGC5431176.1"
                     /translation="MFALINQGQLYTDSSGYPIKIIRCINSTVLYRRMDGLTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            14046..14156
                     /locus_tag="ACIYSB_004259"
     CDS             14046..14156
                     /locus_tag="ACIYSB_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431177.1"
                     /translation="MRQRYGNRYDLTRDLEGYYCREVVKRMFEVWCHCRG"
     gene            14175..14390
                     /locus_tag="ACIYSB_004260"
     CDS             14175..14390
                     /locus_tag="ACIYSB_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGC5431178.1"
                     /translation="MSLNIDCVPITTWCRDMGETLDAVNKRLQRGVWEEGVQVLNVEG
                     SKERWIDLTEVSKWARKNKYPYHSQEE"
     gene            14345..>15053
                     /locus_tag="ACIYSB_004261"
     CDS             14345..>15053
                     /locus_tag="ACIYSB_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HGC5431179.1"
                     /translation="MGEKKQISLSLPRGITIRQYKNCSTLVVTFTYKGVLCREPLSRL
                     EVNAKGIKYAERLLGEIKNKISDGSFDFSEYFPNSKKLILFGMVKRNKSVKDYLDEYL
                     EICKNRKLSPSTINGYEKCVSALSELHNLHVIDLTPAILKKWISRRTTKLKTIRNNLS
                     FLRSAVDEAVTDGLITINPVKLVSASRYHAVNHTTNSAEYEVDPFTPAEAAAIYNACI
                     YEEWRNLFCFAFNTGLRS"
BASE COUNT         4091 a         3716 c         3390 g         3856 t
ORIGIN      
        1 ccttgcgacg gtcctctttt atcttgaagt acagattcgt cagataagtc agaaagccca
       61 gtgccagact ccccagcaca ccaatagcag cccactgtga cggactgacc tgatcaatcc
      121 actgtaaaaa ccagtacccg gcactacctg ctgaggtgcc ataggcaaca cccgttgtta
      181 acttatccat ggatttcata accccacctc gcagatgcgg gtgctgtgta atggaaataa
      241 aaaggccacc tgacgtggcc accagattat ttccccacca gctcgtttat ctctttcact
      301 gtctgattaa accgctctga ctcaagctca acacctaagg cccgacgccc cagcgccatt
      361 gctgctttta ttgtggaacc ggatcccata aaaaaatcag caaccagatc acctggtcga
      421 ctactggcat tgattatttg cctgagcata tccgccggtt tctcacacgg atgtttaccc
      481 gtgtagaact gaacgggttt atgcatccag acatcggtat aaggcacgga gactgatacg
      541 gaaaaatagc gccggagaga tttaaactca tccagcaatt cagaatattt gcgattcagt
      601 gaatcataag atgccaccag ctggtggtgt ggttgttcca gttgttgttc ctgaaacttc
      661 tctgccgcta tacgggaaaa cagtgcctgt aacttccgat agtcagcctc attcggcaac
      721 tgccactgac tggcaccaaa ccagtgggaa accatatttt tcttacctgt ggcttcggca
      781 atttgttttg ccgttatacc cagttcggca cgagcatccc tgaaatacga aatcagcggt
      841 gccattatgt gctgtttgag ttccctttct tttgctgcat agccgtcact tttgccgcga
      901 tatggcccct ggtaatgttc agcaaacaga acgcgctctg tggcaggaaa atatgcgcgc
      961 agactttctt tattgcaccc gttccatcgt ccggacggct tcgcccagat aatgtggttc
     1021 agcacattaa agcgttcacg catcatgatt tcggtatcag atgccagacg atgaccgcag
     1081 aacaggtaaa ggcttccggc aggttttaac acccgccaga actgcgccag acactgatcc
     1141 agccatttca ggtaatcatc atcgcccttc cactggttat cccagccttc gtgtttcact
     1201 ttaaagtatg gcgggtctgt gacaatcaga tcgacagagt tttccggtaa ggtctggata
     1261 aattccaggc aatcagcgtt gattaactca caactggata tttttacagt attagccata
     1321 gatcaataag cgcttctctg ataggctcat accgcttttg cgcaaagcag atgggcctga
     1381 ggtttgcttg tgaccccaac gcatgagcag atggctggca ggtgccgcta acacccacca
     1441 gccgcccatt accacaaatt aaaaagcctt cactgcggaa ggcgtctgta acaaccgaac
     1501 tgataatctg ccaggcccgc cataacaagc tgggtcagta ttaactggca gcattcgcgt
     1561 gaaaggtaag tattctgcgc aatctccccg acggtcgccg gttcggtgac gcttaattca
     1621 ttaagcacca ctctggcggt ttctgtcata tcttgctgtt ttagcatgtc tttttccctt
     1681 ctggttaaca tgacatacca ataactcttg tctaaaaagc cagcaagctg aaagacctgt
     1741 attcgcaacc accagcgcgt ttaacgtcct gtgctgtttt tcggacacaa aaaaacccgc
     1801 acggcggcgg gttgtaaaaa atcttttaac gtcaggtata aaacgcccat cgttagtgcg
     1861 aatttaccac agattcggga aaaatcaaca acactatcgc gttaccctct tcaactgccg
     1921 ctccgcccat gactcttcaa tgtcaaaccg aaccaccagc gtatcgtaaa aacgcttcac
     1981 tgattttttc cacgtatcaa gagtgatagc gctcgtcact ttgcatatgg cattaaacgc
     2041 ctctgttgac ggtaatcttt catagccacg accaccacaa cgctggcagt ctctgataac
     2101 aggcatacca cgttttaccg actcttcacg atgaatggcg acaccacgcc cacggcaatc
     2161 attacaggcg gtggagacct caccctttcc cccgcattca ggacaggcaa cttttaccac
     2221 ttcccggact tttttccatt cttcccagta agacggatac acaccctttg tacactttgc
     2281 ccataccggc ggtttaccat ccggatactg gaccttgttt gtaaaaacta cgctttcaat
     2341 aaattttttc ccaccgcaac aagggcactg ttttttactc gctgcactac gggcataatc
     2401 ctcaaaagcg tacgaagcca taatgcgcat cactgtcggt tttatttctg ccggaagttt
     2461 tctcaacgcc gccacgcgat cgcaccgact gagtgcataa tctgccagta attctgttgc
     2521 ccgcgttctg tcattcatac tgatgcccat tttccccagg aacgcagaaa atcccatctc
     2581 agcccggttt tgtgtcatac cctgcgcagc catcacatca gtgatgctca gcgcatcttt
     2641 tgacgtcgag acggatgcat cagtcaggcc tggcgatttt ggggaatagt atttcggtaa
     2701 atcttccagt ttcatttttg gacctccccg tcatgcatta tttcgtaaat cttcacgccc
     2761 agccgcccac caggaacagg ctgaccgcgc acaatattga tttcatcaaa ctgctcgtcg
     2821 tctatgagaa gtccggcatg cgtcagcgca tccagtggtg ctttcaggat attgtccagg
     2881 tcacgacggc gcttatccgg tggctctgca ataatcttta tcgccagcct tccggacagg
     2941 tttaatttca gccgctgctg gcgaacaata agtgccacat cacggcgata acactcaccg
     3001 gcttttgata caaaatatgt gctgccacga cgtcgccagt aggtgttcac cgtcggcggg
     3061 taaggtaaaa taaactcatg gcgcatcagc gcagcacctc ctgcaccagt ttttcaaact
     3121 ttccgacttt ggtttccagg tcagccacac aatccaccag ctcatccact gctttttgcg
     3181 tgcgatgttt cgcctgcatc aattccctga gcgctggcac catatcccga cgaatggcat
     3241 cttttgttac acctgttttt tccagttgtt ccgcctgtcg caacatttcc tgcgcctgct
     3301 tacgtaattg ttcaggggta aaagttattg cctggttatt caaaataaac gctccgtttt
     3361 acttcccgat atgcggctat tgctatatct gcggggactg cctggcttca caggagttga
     3421 aagcacccgt gcactttcct ggtccacggg cagaaaatgt ccgttatgaa atcgtcggta
     3481 aatggtccca agagtgccat tacgctgttt cgtgatatta atttctgcaa tgcccctggc
     3541 ctgcgtatcc gggttataaa cttcatccct gtaaagcatc agaatgatgt ctgcatccgc
     3601 ctctatttcc ccggaatttt tcaggtctga gttcatgggg cgtttattgg gtctggactc
     3661 cacactgcgg gaaagctggc tcagcgcaat caacggaaaa ccacctgatt ttgccaggcc
     3721 tttaagtcct tttgatattt cccccacggc aagatcatga cgccccgtgg ttctggtttt
     3781 tatcagtcca agatagtcaa ccaccaccag ggccgtttcc ggatgtttag tcagatggtg
     3841 tttcgttgtt gcgcatatct cgtcaatagt caggttcgcc tggtccacca tccagatatt
     3901 gcgaccggtc atccgtccca ctccttgtga gaaacgcgcc cagtcttcgt cttcaaagtg
     3961 agcgacagat ttcagacgtg ataccggcat tcctccagcc gcagatacca tgcgctcacc
     4021 aatctggatg ttcgccattt ccattgtgaa cagaagaaca ccatgcccct gctcagtcac
     4081 cttgtcgatg atatccagcg ccagttcggt tttgcccatt gacggacgag ccgcaataaa
     4141 taccaggtcg cctggctcca tgccgcctgt ttttgcgtcc agttcatcaa taccggtcat
     4201 caacgtcctg gacttctcca gcccctgatt ccggcattca acacgttcaa ccacttccgg
     4261 aagcacatca tcaatatgta ccggctgaat agcgcccttt tccatcgaca atgaggccat
     4321 catgttttga gcatccttca gggcatcctc ggctgcttca caggtatgtg catcacgtaa
     4381 attctgtaat gcttcggtca gtgttttttc tgcatcgcgc agtgcggcat ttcgccgcag
     4441 cgctgcaaca tagtgctcca gtgaagactt cacccaggtt ttacgcccgg tgtcggtaat
     4501 caccggggca agttccggca tctcattgca cagcagcacg gggtcaatga cgccggatac
     4561 gcgagcctgt ctgcaaatcc ccgcgtaaat atccctgtac tgacgcacga aaaagacatc
     4621 cgccggaagc gtggccagaa tatccatcac ttccggatca gccccacgca gaaaaaacgc
     4681 gccaatcacg gcaccttcca ggtcatcgtt acgccatgcc ggatttgtcg gttttgtcat
     4741 gctgccacac ctctgatatg cgcacggtag ctttcccaac caaacaccag gcagttacac
     4801 ccaccatcag taacacgatc cacaatccgt tcaccaatgg attctttaag ctgttcaaac
     4861 gtcaggttgc tgatcaaaat tgtcggtaaa acgctttcgt aacgcgcatt tatgatttcc
     4921 tgcaggatag ttatctctgt aggcgtgccg aactgcacac caacctcatc gatgataagc
     4981 agatccagcg atgcaaaatg attaatcact tcatcgtcag tgcgctcaga gttgtggcgc
     5041 caggtatttt tcaccgcacg ggtaagccgc atcacatccg tgatttctat ggttgcctgg
     5101 tggtggcgaa taatattttt tgccattgat acagccaggt gatttttccc ggtaccacaa
     5161 ttgccaatca tgaccatgct ggtgcctgca gcgagacatt tcttccagga agcggcatag
     5221 cgctgacagg cttcaaggtt tttctgtgcg tcagtattca ccgcctgata attctcaaac
     5281 tcacagtcct gaaaccggcg ggcgataccg gcctgatcga gtaattcacg aaccttcagg
     5341 gcgcgtaatt cgtcatacac gctgtccagt tcctcactga ggcaggacag gcaaccggac
     5401 acccgtttga cagctttccc cctgacattc gggcccgtca gcacatagcg tgtatatttt
     5461 ccgtgtttcc cgcaggacac cgtctcagaa ctttgcaccc aatgctcaca gcgccacgaa
     5521 cgctttccgc cacggacaaa tgccagttcc tcttccaggt cagccctgcg gttgagtaac
     5581 tgcgctttgt cgtattgcat gtgttgtttg ttcaggaaat tagtcatttt cacaccccac
     5641 aaaatcacca gttaaagttc gttgagccgt agtcctgctc actgaatccc gagatcggga
     5701 ggcttttgcc ccgcccacct ccgggagcgg ctggctgttg ccagaagtct tcgaagtgac
     5761 gatcggggcc aaagaacgtc gcagcctgct tcacgaactg tgtgccggta tttcctgtcg
     5821 cacgtaccca ggcggcatac cccttcacgc catcaagcat ggtctccggt tttattccct
     5881 ccctgatacg ggctttccag gctttgaagg ctgctgactt ggaattacca ccagcacgtt
     5941 tgggatattc ctgccaggcc tgttcaaatt ccggtgaata ttcctgccgg gcagagcgcg
     6001 ctggtgcaga cgcgtcagcg gatgcgccaa tagtgttttt agtctccgtt gtaatctctg
     6061 tagtaatctc tgtatttgta tcaacattcg gcgtatcccc tgttccgtta tgacgttggg
     6121 gggtgttccg ttttaacgta atagctgtag ctgtgtcgct gactgtgtta ttgctgttgc
     6181 tttctgacga aacagaagaa ggtgtggtga tggcagcaac tgcctgtggg ttgatcccga
     6241 caaacaaaat attgctgcat ttcacaccat cgagcatttc cacagtgcgt aaatccaggg
     6301 taataaaacc ggcatcccgc agacgcttca gcgcatctgc agtttccctt ttcccgaaac
     6361 caaactgctc agcaaacgcc tgatagcttc tttgcagctt gtccccctga aaacgcttgc
     6421 gatatcccag caaagccccg gtatgctcat cccggacttc tgtcgggcgg taccaataaa
     6481 cgatctctga gagcagggcg atagcggtcg catccggacg accactgggt agccgaatat
     6541 gtttccacca gttcgctggt gtgacattgc cggaaatatt aatctgccca atagccataa
     6601 cttccggtgt gggggcgtaa cgactcatac agcctcctta cgcggcataa caatggtgta
     6661 accccgggca ggctggagcc gggcttttgc atcaatagtg agcgttgcaa ttttacggat
     6721 acgcagatac cccgcccttt ccagggccag ggtttctctg aacattgcct gttttgaaca
     6781 gcaacaaaaa tcggcaagga cctggtgatc aataaccctc tcaccttcac cgtctgaaga
     6841 acccgacatc aaaacacgca acataatcag gcgctgaatc ggattatcga aaacacttcc
     6901 gcatacaaac tgaaaacagt tcacacagca cctcccaggc gtttaaacat ttttccggac
     6961 tgaaacgcca ccagcggata actcagggta tgagtacgtc cgtgaacctg acagacaacc
     7021 ttctggcttt ctgtattgac caggcaaacc cgcagaacgt ggccgttgct ggtggtgaac
     7081 cactgcccca cacgggggca acggttgtat cggtgataca ggaaattaac gatgcggcga
     7141 atcatggatg tacctcctgc acatcagcca tgatcatttt gctggctgtt tccagcgccc
     7201 ggtcggagct aatttgcatg acagccagtg aatgaggaac gaaagccccg gcatattctg
     7261 tttcactggt ggcgtgctta tgcgccctgt ctgcaataac agagatgtca ataagcgcat
     7321 ccatcagggt tttaattacc gtagcggccg tctctgaata atggttatta cacatggcga
     7381 gcctcccgtt gattacgacg gaaagtggag ttattcaggc tgttctctgc ctcatggaat
     7441 gcttcaaggc agctctcgta gtaccgcatt gtgcgtagac ttaacccaag ctgaagcatc
     7501 atcaggccat caagggtgat gtaataacca cgcagggagt caccatagat gtggtaagta
     7561 cccggtataa agttgcgggt aaaaaattcg cgcgagcagt tcagatactc gattttgtcg
     7621 acgatgttct ggtgcatgcg cttaaagtgg caggcaacat gcagggagaa aataacagct
     7681 ttgccgttga cgacttcgat tttgaggaat ggggaagttg ggactgtagc catgatggca
     7741 gcctccttga tcggtgaaaa acttccacca ccggagctgc gaaactcatg ggtggtgaac
     7801 tgaacagggt tcgcagtacc ggcgatcaag gaaaccggcg agcctttcgg ctcccctgcc
     7861 cagcccacca taattctggc gtgcatgagc gcagacgata aaaaagacgc tggcgcgtcg
     7921 tatatcgcct tgatcaattc cgggctgcga cccccggcac ccgctttata aggtgctgga
     7981 acagtgtaac gtcccgaaat tgcagaatca atatttaggc ttgaaacatt catatgctta
     8041 ctggtatttt tactaacgca aatgttcttg gatgttactg gcttacttct ttgtgaagtt
     8101 gcctttacca acgttgagcg agccgggtca ctacccggct ttttttcacc gctgccaacc
     8161 aataacctga aataacccca ttttcgggtg ataccagcga gtccctcgcg gttctgcttc
     8221 ctccattacc cgataaaaag cagccataaa cggttccaca gcaacaattg cgcgacgtga
     8281 caacaatccg tccggcgtca tgaactcgtg ggtgtctgta ggaatttgat aagcgttcac
     8341 cagattgcgg catttatcat ctgacaaacc ggttttcgct ttcagttggc gatatccggc
     8401 atagccctca cgaatggtgc cctttttgat ttgctcaact gtttcggcaa cgtggctgac
     8461 tttttcttcc acctgagtga tccgtttctg ttggcgaacg gcttcaagag ccatcgcggc
     8521 aaccatttcg atttcgctca ttggcttacg gatctgttct tccagttcgc gccagcgatc
     8581 taccaggcga gcagtgaatt caggacagag ctgtgcgacg acaatgatgc tgtcgcgctt
     8641 accttgttca ccttcgaata catacgcgct agaaaatcgg cgaggcccaa gcgattgttt
     8701 attctcaatt tccacagtct gtggaaattg aatgattccc tttttagcca gtgtttcaat
     8761 agttctctta acactatctg gtcggcttcc caccagctca gctatctcaa tactggtcat
     8821 ggatgctttg tcgttaaaaa ttgcggtgtt cattgttggt ctcctgtggg cttgtcatcc
     8881 tctgtgtgcg ctaagcttgg atgtggaaaa agctctggta gatcagggcg aaattcatgc
     8941 gcctgaatct ttccatctac tgctgcaacc aataacggca caaattctgg tgaaatcttg
     9001 ttcttgcagt tcaaccaatc gcagactgtt gactgggcct ttccgcatcg cttggctaag
     9061 gcttgctgac ttccaagaat ctcaatagct ttctctatgg ctttatgttt cataatcgca
     9121 tctcctattg aacgcataat aacaacagga aaagcgatat tcaacgttaa acctagaaca
     9181 tttatcggaa agccgataga ctagccaaga tagagatggg agggaaatcg caatgacatt
     9241 ttcagaacga cttgatttag ctatgcgcaa cgcaaaattt acgcaaggta ggctggccaa
     9301 agaagtgggt atggctcagt ccagtgtcaa tcaactactt aacaaagcca acggttccag
     9361 aaaaacagtc gaaatagcaa aagttttggg cgttaacccg gaatggctcg catcgggtgt
     9421 tggcccaatg gaaatcgtga cctctgcaga ctcacaccaa atacgtaaca tttctgagga
     9481 ttgggttact gattcgtatg tcgtggatgt tttagacatc agatatagtt gcggaccagg
     9541 tagctataac tctgactatc ctgatattgt tagatccata gcgatagaac caggatatgc
     9601 atcaagaatt tttggcggca gaccagcatc agcaatcaaa gctataaatg cccacggcga
     9661 cagcatgaaa ggcacaataa atcctgaaga tttggtgttt gtagacatat cagttcgctc
     9721 atttaatggt gatggtatat acgcttttac atactctgga acctcacaca tcaaaagact
     9781 tcaaaaagtc aaagataccc tgacggtgat ttcagacaat ccagcctaca aggattgggc
     9841 tatcgagcag gctgatttcg agcaacttca cattgacgga aaagtaatcg tcagttggcc
     9901 catgacgcta caccgtttcg cataaacgaa aatcatttag catacccgcc cgctgacaag
     9961 tgggcttttt tttaaaaaaa aaatcgataa accgattgac acaacaaatc ggtaaaccta
    10021 ttattatgac ataagtctcg aacttaccaa agcaattata gaggatgagg aaatgtcaac
    10081 gtatatgggg ttaccaacaa atcagcacga tgctcttgaa aatattcagc ttttagtagg
    10141 ggccggagct ctactgtata gcagtaataa cccggagtta attgagcttg ccagctccat
    10201 cctagatgta actcaaaaat atagcctcaa cgcggcgcgt tcacgcaaac aaaatttagc
    10261 gattaactca cagaaccgaa ttatcaccca acgcgaagcc tgcggcttaa caaccgccga
    10321 actcgccagg ctgcttgatc tcgatgaaga aattatcatc cagtgggaga gcggagagta
    10381 cgaaccaacc atcagcatgc ttatcccact ggctaacgtc ctgggctgcg atccgctttc
    10441 tctgctgagt gaaaacaata ccgcagtacc tgttcgcgta aatgtgccag aagtcaatgc
    10501 ggaaagtatt ggcgcacgca tcgaaagtgt ccgtaaaaaa ctggggttaa ccgaatcaga
    10561 tcttgcccgc atgattcata cctacagcga ccctataaac gactgggaat gcggcatctg
    10621 cgaagtccct gctgatcaga tagtaccact ggccagcgcg cttcattgtg acccgatgtg
    10681 gttgttaacg ggtaaaccaa cagatggggg tacctgcgca tgaaaaagtt cgaaaacata
    10741 accgttctct atgttgatga gtttgattat gcaaaccagg aatttctccc ggaggttgta
    10801 aaggcaatag atgttgccga tatagtgatt agaggaaaga gaattgtcaa aaacagactc
    10861 gcatgcactt ccaggagcaa tgacagaaaa acctcacagc aagataacta cgaaggcatt
    10921 tgtctggagc ctgattcatt tgcggtaaat atttatcatt tattgcatgc aacacaggta
    10981 ttacatatgt ccagtaatta cgaaacgaaa acactcggca gcgaaattct gaattttgca
    11041 tgtgagtatg caaaagctgc tgccgaaaaa gaattagcgc aataacataa ataccccctg
    11101 aacgtttatt acggttttat cgccggggat tggtacaacc tttatttata gagaaaactc
    11161 aattataact ttcctgagac ataaggcatc gtacaaaact gcctgcctca ttgcacaaca
    11221 tggagattct tattttcata tagctaacct gtatttacgc aaagcatatg ggagataaat
    11281 caatggaaga aaaagaacaa aacataacac atgaaaaagt cagagtgttg ttgaccattg
    11341 aaaatgggaa ggtaattcac tcaaaacatc tgttggataa tgaatttgtt ggctgcatag
    11401 atacatttct gtggatggca aaaagagcgg gttacacgat tattccacca gcaaaggagc
    11461 aaacattatg aatcattcag agttccgacc agaagttacg ccacacggta taaaaattgg
    11521 caatacaacc attgattatg ccgaggccgt acagcgactt aatgatggtg aatatgatta
    11581 tccatgctgg cacggtttaa gaatcatgca atgtattgcg gaagccgatg atgccggatt
    11641 gctgggaaga ttttcagtcg atctgaaggt tgcccagtgg cgctggctgt atgtggcgac
    11701 gtttatcagt gaagaggaag acaagaacgg taccattgat atccctaacg ataacggaac
    11761 tacagatcgc gcagttattt ataaggggaa gcatggttgc ctgagtatat acccaggacc
    11821 acttcgcatt gccctgcaaa accatgtcga atggggattt attgaaaaat atggcgaagc
    11881 tgaaggcatg gggcgagttc tgtttctcta tcaaaaaatg ctcatcgcag atcctgataa
    11941 tggtttcatt gtctctgcta tgggacgaga agggcttgaa ctccttctgg atgaaatgac
    12001 tcacgatctg aatactcatg gtataccaga agcaccagtg acacattaaa tattacatat
    12061 ggcaaatcag aataaaatta atgtatttca ggttgaatca aaacataaaa cacctgtgat
    12121 aaaacacgtt cgccgccata cacgcatcta tacgccagaa gagtttatag caatgccaat
    12181 gataaagaag tttattcgtg ataatcccga tcacattgct atagataaaa ataatggaga
    12241 aataatgtta tcacgcgaac ttgccgaaat ttactataac gtgaataaca gtaaaaagtt
    12301 gaaaaaggca atcagaaaga tatcaggagg agcatgaata tttatttcag aatagttata
    12361 tcactggcaa ttatcgcatg catttacgga ttactcgtcc cgtttctgat atcaatgaag
    12421 gatacggtag cggtaatttc tggctttgca ctggcgtttc tgaccccgcc atgcatttat
    12481 gccatttata aggggctttc tttcactaag gataaaagat gaaaaaaatt attttttctt
    12541 tagccattgt tctgcctgcc attggccttg tcggttgtga tcgtgttgag cctggtaatg
    12601 tgggcatcaa agtcaacaaa cttggcgacg ataaaggcgt aggtgaagtg gtcggtgttg
    12661 gtcgctactg gacaggctgg aacactgaag tttatatctt cccgaccttt aagcagatga
    12721 agacctacga tgatccattc agtttccaga tgagcgatgg aacaaccatt ggttaccaca
    12781 tcggagtagc ctacaaggtt gatccatcca aagttaccac ggtgtttcaa acctaccgca
    12841 aaggtgtgga tgacattacc gacaccgacc tgcgccagaa gatagcagat gcactaaatc
    12901 ggctggccag caaaatgacc actgataaat ttatcgacgg tggtaaatct gaactactgg
    12961 attcagctct aaaagatatt caggcagaaa tgacgcccat cggtattcag gtaatgagcc
    13021 tgtcttatgt aggtaagccg gaatacccgt caactgttat tgacagcatt aacgctaaag
    13081 tcacggcaaa tcagaaaact cttcaacgtg agcaggaagt aaaacagcgc gaagcggaag
    13141 ccaacatgtt gcgcgcggaa gctgccggac aggctgatgc gattcgcaca aaagcccagg
    13201 ccgaagccga tgctattcgt ttacgcggcg aagctctgcg ccagaacccc ggtgttatgg
    13261 agctggaagc catcaataaa tggaacggca cactgccgca atacatgacc aacggtgcca
    13321 gtacaccatt tatccaggtt aaataactta tatgcccggc aagccgccgg gctaagggaa
    13381 agcagatgaa cactcataat actcaaccga aaataatgaa ctatgacccg aatctgacgt
    13441 cgtgcggacg catggcaaaa caaaccgttc gattaacttt cggactatgg gaataccgcg
    13501 aaacattcga agtcactgtc ggtggcaacc tgaccggact cgatgttatc agttgcgcta
    13561 ttgaaagcct gtacgcaacg ctgccttatg aagaagtcga ggatgagcgc gatatcatag
    13621 ccaccattaa tatcggcgga ctggaatgca aggatgaaga cctgcaggga gaactctggc
    13681 ttgccgagat gcttgtatcc gcagaaataa tcagcattga gcctgacaca ggcatacaac
    13741 tctgaaattc cataaattca gggagcaata gaaaaatgtt cgctttgatt aatcaggggc
    13801 agctatacac cgacagttcc ggttacccaa taaaaattat tcgctgcata aacagcactg
    13861 tgttgtacag aagaatggat gggttaacac agtcggtaaa aataaacgat tttaatgaac
    13921 tgtttgaacg gatcgatcac caggaatacc gacaaattct ggcagaaaca gagcaggaag
    13981 ctcatctgaa aaaattacga gccatgaaaa ggaagtaaag aatgaataaa gcatttgaac
    14041 gatgagtgcg ccagcgttat ggcaatcgct atgacctgac gagagatctc gaaggctact
    14101 actgccgtga agtagtgaaa cgaatgtttg aggtgtggtg tcactgccgc ggataaaagt
    14161 tttgtgaggt aactatgtct ctcaacattg actgtgtacc tattacgaca tggtgtcgtg
    14221 atatgggaga aactctggat gccgttaaca aacggttaca acgtggagta tgggaggaag
    14281 gggttcaggt tttaaatgtg gaaggctcca aagaacgatg gattgattta actgaggttt
    14341 caaaatgggc gagaaaaaac aaatatcctt atcactccca agaggaataa caattcgtca
    14401 atacaagaat tgctctacat tagttgtgac cttcacctat aaaggggttc tttgcaggga
    14461 acccttatca aggctggaag ttaacgcaaa ggggattaag tatgctgaac gactgctcgg
    14521 agaaataaaa aacaaaatta gtgatggctc attcgatttt tcagagtact ttccaaactc
    14581 caagaagctt atattatttg gaatggtaaa gaggaataaa agtgtgaaag attacctaga
    14641 tgaatattta gaaatttgca aaaacagaaa gttatcccct tcaacaataa atggatatga
    14701 aaaatgtgta tctgcattat cggaactaca taacttacat gttattgacc tgactccagc
    14761 aattttaaag aaatggattt caagaagaac aacaaaactg aagaccatca gaaacaattt
    14821 atcattttta agaagcgcag tagatgaggc tgtcactgac ggacttataa caataaaccc
    14881 tgtaaaacta gttagtgcaa gtagatacca tgctgttaac catacaacca attctgccga
    14941 atatgaagta gaccccttta cgccagcaga agcagccgct atctacaacg cctgtatcta
    15001 tgaggaatgg cgaaaccttt tttgtttcgc attcaataca ggccttcgaa gct
//
LOCUS       DBBGLQ010000107        14972 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.107, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000107 DBBGLQ010000000
VERSION     DBBGLQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.107"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..701
                     /locus_tag="ACIYSB_004262"
     CDS             <1..701
                     /locus_tag="ACIYSB_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGC5431180.1"
                     /translation="LRHKPKLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYE
                     QGEIVINGEKIMRPDYGDMLKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNG
                     VLQWSTIRRLTEEVMQRMTVKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEP
                     TRGVDIEAKQQIYRIVRELAAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSP
                     VNVDELMSAILSVH"
     gene            726..1724
                     /locus_tag="ACIYSB_004263"
     CDS             726..1724
                     /locus_tag="ACIYSB_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5431181.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            1757..2851
                     /locus_tag="ACIYSB_004264"
     CDS             1757..2851
                     /locus_tag="ACIYSB_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGC5431182.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            2863..3735
                     /locus_tag="ACIYSB_004265"
     CDS             2863..3735
                     /locus_tag="ACIYSB_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGC5431183.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNHFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(3783..4205)
                     /locus_tag="ACIYSB_004266"
     CDS             complement(3783..4205)
                     /locus_tag="ACIYSB_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGC5431184.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(4302..5510)
                     /gene="hcaD"
                     /locus_tag="ACIYSB_004267"
     CDS             complement(4302..5510)
                     /gene="hcaD"
                     /locus_tag="ACIYSB_004267"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGC5431185.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLPLLPPPWFWSDQYSDNLQFIG
                     DMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLL
                     IDENIALKSL"
     gene            complement(5520..6332)
                     /gene="hcaB"
                     /locus_tag="ACIYSB_004268"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(5520..6332)
                     /gene="hcaB"
                     /locus_tag="ACIYSB_004268"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGC5431186.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(6329..6649)
                     /locus_tag="ACIYSB_004269"
     CDS             complement(6329..6649)
                     /locus_tag="ACIYSB_004269"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGC5431187.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(6649..7167)
                     /gene="hcaF"
                     /locus_tag="ACIYSB_004270"
     CDS             complement(6649..7167)
                     /gene="hcaF"
                     /locus_tag="ACIYSB_004270"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGC5431188.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(7164..8525)
                     /gene="hcaE"
                     /locus_tag="ACIYSB_004271"
     CDS             complement(7164..8525)
                     /gene="hcaE"
                     /locus_tag="ACIYSB_004271"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGC5431189.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            8661..9551
                     /gene="hcaR"
                     /locus_tag="ACIYSB_004272"
     CDS             8661..9551
                     /gene="hcaR"
                     /locus_tag="ACIYSB_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGC5431190.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLNGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            9711..10850
                     /locus_tag="ACIYSB_004273"
     CDS             9711..10850
                     /locus_tag="ACIYSB_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGC5431191.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAVGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(10842..12122)
                     /gene="csiE"
                     /locus_tag="ACIYSB_004274"
     CDS             complement(10842..12122)
                     /gene="csiE"
                     /locus_tag="ACIYSB_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGC5431192.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGMTPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12313..13167)
                     /locus_tag="ACIYSB_004275"
     CDS             complement(12313..13167)
                     /locus_tag="ACIYSB_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGC5431193.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKLYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYHGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(13312..14115)
                     /gene="suhB"
                     /locus_tag="ACIYSB_004276"
     CDS             complement(13312..14115)
                     /gene="suhB"
                     /locus_tag="ACIYSB_004276"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGC5431194.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            14234..>14972
                     /gene="trmJ"
                     /locus_tag="ACIYSB_004277"
     CDS             14234..>14972
                     /gene="trmJ"
                     /locus_tag="ACIYSB_004277"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGC5431195.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
BASE COUNT         3400 a         3788 c         4168 g         3616 t
ORIGIN      
        1 cgttacgcca taagcccaag ctggaggata tcagctttac gctacgtcgt ggcgaagtgc
       61 ttggcattgc tggcctgctg ggggcagggc gcagtgaatt gctgaaggcg attgttgggc
      121 tggagacgta tgaacagggc gaaattgtta tcaacggcga gaaaatcatg cgccccgatt
      181 acggcgacat gctgaaacgc ggcattggct atacgccaga aaaccgcaaa gaagcgggga
      241 tcattccctg gctgggcgtt gacgaaaata cagtgctgac caatcggcaa aaaatcagca
      301 ccaacggtgt gctgcaatgg tccaccatcc gccgcctgac cgaagaggtg atgcagcgga
      361 tgacggtcaa ggctgccagt agcgaaacac ccatcggcac gctttctggt ggcaatcagc
      421 aaaaagtggt gatcggtcgt tgggtctatg ccgccagcca gattttgttg ctcgacgagc
      481 caacgcgcgg cgtcgatatc gaagccaaac agcagattta ccgtattgtc cgtgagctgg
      541 ctgccgaagg aaaaagcgtg gtgtttatct ccagtgaagt ggaggagttg ccgctggtgt
      601 gcgaccgcat cctgttgtta cagcacggta cgttctcgca ggagtttcac tcgccggtca
      661 atgtggatga gctgatgtcc gccattctgt ctgtgcactg atgaatttta ccgaggaacc
      721 tgaagatgtc tgcttcgtca ttatcattgc cgcagggcaa gagcgtctcg ctcaaacaat
      781 ttgtcagtcg ccatattaat gagatcggtt tgctggtggt aatcgccatt ctttatctgg
      841 tcttctccct gaacgcgcct ggctttatct cattgaataa ccagatgaac gtgctgcgcg
      901 atgccgccac cattgggatt gccgcctggg cgatgacgct gattattatc tccggtgaaa
      961 ttgatgtcag cgttgggccg atggtggctt ttgtctcggt gtgcctggca tttttgctgc
     1021 aatttgaagt tccgttggcg attgcttgtc tgctggtgtt gctgttaggc gcgctgatgg
     1081 gaacgctcgc cggggtgctg cgcggcgtgt ttaacgtacc aagtttcgtt gccacactgg
     1141 ggctgtggag cgccctgcgc ggaatggggc tgtttatgac caacgcactg ccagtgccga
     1201 ttgacgaaaa cgaggtgctg gactggctgg gcggacaatt tctcggtgtg ccagtatccg
     1261 cgctgatcat gatggtgttg tttgcgctgt ttgtgttcat tagccgaaaa accgccttcg
     1321 ggcgctcggt ttttgccgtt ggcggtaatg ccacggcggc gcagttgtgc ggcatcaacg
     1381 ttcgtcgggt acgcattctt atctttaccc tttcgggatt attagcggcg gtgaccggca
     1441 ttttgttggc ggcgcgcctc ggttccggta acgcaggtgc cgcaaacggt ctggagtttg
     1501 acgtcatcgc cgcggtcgtg gtcggcggta cggcactttc cggcggtcgc ggttccctgt
     1561 tcggtacatt gcttggtgtg ctggtgatta cgctaatcgg taacggtctg gtgctgctcg
     1621 gtattaactc ctttttccag caggtggtac gcggcgtcat catcgtggtg gcggtgctgg
     1681 cgaatatctt gctgacccag cgaagcagta aagcgaaacg ctaaacctta acgcccctgg
     1741 cgcgggtgcg gcagggatga aaattaatcg tattgttaat gagggattta tgaaaacgat
     1801 gctggcagct tatttaccag gaaattcgac cgtcgatctg cgggaagttg cggtgccgac
     1861 gccggggatt aaccaggtac tgatcaaaat gaaatcctcc gggatttgcg gaagcgatgt
     1921 ccactatatc tatcatcaac accgtgccac agcggcggca cccgataaac cgttatacca
     1981 gggctttatc aacggtcatg aaccgtgcgg gcagattgtg gcgatggggc aaggctgccg
     2041 ccattttaaa gagggcgacc gcgtgctggt gtatcacatt tctggctgtg gtttttgccc
     2101 gaactgccgc cgcggctttc ctatctcttg tactggcaaa ggaaaagcgg cttacggctg
     2161 gcagcgtgac ggcggtcatg ccgaatatct gctggcggaa gaaaaagatc tgatcctcct
     2221 gccggatgcg ctgagctacg aagatggtgc gtttatcagt tgcggcgttg gcacggccta
     2281 tgaagggatt ttgcgcggcg aagtttccgg cagcgataac gtgctggtgg tcggtctggg
     2341 gccggtcggc atgatggcga tgatgctggc gaaaggtcgc ggtgcaaaac ggatcatcgg
     2401 cgttgatatg ctgccggaac gtctggcgat ggcaaaacag ttaggggtga tggatcacgg
     2461 ctatttagcg accaccgaag gtctgccgca gataattgct gaacttaccc acggtggcgc
     2521 ggatgttgcg ctcgattgtt ccggtaatgc cgcaggtcgc ttgctggcac tgcaatccac
     2581 tgctgactgg gggcgggtgg tttacattgg tgaaaccgga aaagtggaat tcgaggtcag
     2641 cgccgatctg atgcaccatc aacggcggat tattggctcc tgggtgacca gtctgttcca
     2701 tatggaaaaa tgcgcccacg atttaacgga ctggaaactg tggccgcgta acgccattac
     2761 ccatcgcttc tcgctggaac aggcaggtga tgcctatgcg ctgatggcga gcggcaaatg
     2821 cgggaaagtt gtgattaact tcccggatta aggagttagc ggatgaccat ctatacctta
     2881 tcccacgggt cgctaaagct ggacgtttct gaccagggcg gcgtaatcga aggcttctgg
     2941 cgcgatacga cgccgctctt acgtcctggt aaaaaaagcg gtgtggcaac cgatgcctcc
     3001 tgttttccgc tggtgccgtt tgcaaaccgg gtgagcggca accattttgt ctggcagggg
     3061 cgtgagtatc aattgcaacc gaatgttgaa tgggatgcgc actatctgca cggcgacggc
     3121 tggctggggc agtggcaatg tgtctcgcgc aatgatgata gcttgtgtct ggtgtatgaa
     3181 catcgcagcg gtgtttatca ctatcgggta agtcaggcgt ttcatttaac ggcggatacg
     3241 ctgacggtga cgctctctgt caccaatcaa ggagcagaga cgctgccatt tggtaccggc
     3301 tggcatcctt attttccgtt gtcaccacaa acgcggattc aggcgcaggc gagcggttac
     3361 tggctggaac gggagcagtg gctggcgggt gagttttgcg agcagctacc gcaggaactg
     3421 gattttaacc agctcgcgcc gttgccgcgc cagtgggtga acaatggttt tgccggatgg
     3481 aatggtcagg cccgcatcga gcagccgcag gaaggttatg ccatcatcat ggaaacgacg
     3541 ccacctgcac cgtgttattt catctttgtt tccgaccctg cgtttgataa aggatatgcg
     3601 tttgatttct tctgtctgga accgatgagc catgcgccgg atgatcatca tcgcccggaa
     3661 gggggtgatc tcattgcgct agcaccaggg gaatcaacaa cttcagagat gtcgttgcgg
     3721 gtagagtggc tgtaatttat taaatgccgg ataaggcgtt cacgccgcat ccggcatctt
     3781 tcctaacgcc gatcgagaga aattgccccc ggcccggtaa ttgccaatag caagaacgcg
     3841 ccagcgatac tgacattctt ccagaaatta atcatatttg gcccaaccgc atcgccggtc
     3901 atatcccagt aatggtgacc aatcaccgcc gtacccagcg tgtagaaaat aaacagcacc
     3961 gccagcggac gggtgaaaaa gccaagcacg attaatatcg cggcgggcac ttccataact
     4021 accgcaataa tcgctgccag catcggcatt ggcgcgccca acgaggccat atattggacc
     4081 gtaccgtcaa agcccatcat tttgggaaaa ccaaaaataa tgaaaattaa gaccacggcg
     4141 atacgggcaa ttaataacaa aacggggcgg gcagctccaa aatcaaaata acgtagtgtg
     4201 ttcataacca atccctttgc aagataatgt gatttaaacg taatacacac tcttgcacct
     4261 cgccacgccc caggcgttga gatattcgct aattatcctg gttacagtga tttaagcgcg
     4321 atgttctcat ctatcagcag tttcgcatca aacgttttgc cgctctggat ccatttgcga
     4381 attgggcgaa tctcacgccc ctgattcagc gtgaccgcac cgataagcac gccgttttgc
     4441 agattaaacc aaatcgcctt ctgagtttcc gggttgccac gacaaagcca gtcatcgcca
     4501 cgcatatcgc caataaactg taagttatca ctgtactgat cgctccagaa ccacggcggc
     4561 ggcagtagcg gtagcggtag cggtagccct aacattgcgg cagcggcaat ttgcgcctgg
     4621 ttattggcgt tttcccagct ttcgcagcgg tgtagtgcac cattatcaag acgagtgatt
     4681 gccacatcgc caccggcaaa gatcgcggga tcgcaggtgc ggcaagcctc atcaatgaca
     4741 atgccattgg cagtatcaag gttggcctcg cgagccagtt gctcgttggc gctgatacca
     4801 ataccgtaaa tcaccacatc agcctgaagc gtttccccac tttgcagcgt cagttctact
     4861 ttttcaccat cgaccacatg ttcaatggca ttattgagca gaatgcgcac accagcctgc
     4921 tggtggcgct gtaaaagata gcgttgcacg ggcggtggtg cattacggcc catgacggtt
     4981 gccgccagtt caatcactgt caccttacat ctgcgctgcg tggcgctggc agccagttcc
     5041 agaccaatag ttccggcacc gataatcacg actgaccgtt cgggctgcag aacttctcgc
     5101 agtctggcgg catcaccggc atggcgtagg gtaaagcagc gttctcccag tgcatcaagc
     5161 aacggcagcg gtcgagctgc cgcgccggtt gctataaaaa gctgatccca gtgccagctt
     5221 tcgccgttgg ttaacactaa ctctcgtgtg tcgcgaccca gcgttttgat ggttacaccg
     5281 gaatgcagat gaacattgtt ttcctgccac cagttagcgg gtaacacctg ctgtaactgc
     5341 ggggaatctt ccagcaacat ggatttcgag agtggaggtc gttcataagg aagatgtcgc
     5401 tcatcggaaa acagatgcag ctcaccggtg aacccttgct ggcgtagcga ggccgcagcc
     5461 attgccgccg cttgcccgcc accgacaata atgatcgttt tttctttcat cgtgcttcct
     5521 taaagatcca gcccagccgc tacgtggcga atgccgcgaa tcgctaaacc cgcatcagcg
     5581 ttgatcatca caccgcttaa tgcgcgatta ttgcgccgcg atgccaacat cacatacggc
     5641 cccgtaaaat ccgccggttg cgggaaaaat tgcagcggta aaatggcggc aattttctcc
     5701 ggcgtcagag actgcattat cgaggtttca ctttgcccga gcgcctgtgg gccgcgcagg
     5761 tcgctggcca taccacacgg gccgacgcca ttcacccgca ctttcggtgc cagttcataa
     5821 gccagttggc gaataagtcc ggttgcggca tgtttactgg cggtgtacag cgggccaccg
     5881 ccgccaggat accaggcggc atttgacagt gtgaaaatca tgctgccttc actggcgatt
     5941 aacgccggag cgcaggcttt tgcgcccagc aggtaaccga gtacgttgac gttaaacagc
     6001 tcgtggaagc cggtttcgag cgtctctgcg ggagtattaa ccagtgaggc attgtgatcc
     6061 cagatgcctg cattgccgat aaaacaatcc agcttgccgg aacgagtcag gatctgatcg
     6121 accgcgcgtt gataatcggc ataacaggtc acgttacctt ccaccgccag aatatgttcg
     6181 ccaaatcgct gacgcagact ggcgactttt gccgccgaca gttccagcgt ggcaacctgc
     6241 gcgccttctt cgataaatcg ctcgaccagc gccagcccta atcccgatcc gccgccggta
     6301 ataaaaatgg actcgttatg cagatcgctc atggctgcgc ctccggtaag tcgatgaaaa
     6361 tgtcaccacc ttcaacgtgt actggataag tggtgagcgg atcggtggcg ggcaggcata
     6421 acgctttccc cgttttcagg caaaaactgg cggcgtgtag cgggcactcc accgtggcgt
     6481 catcttccag atacccttct gacattgacg cattaccatg gctgcaacga tcgttaatgg
     6541 cataaaactc gccgccaacg ttgaacaggg cgatgacggg cgaggtatcg atccggagag
     6601 cctcaccctc cggcacatcc gcaacgggac acgcataaat tcgattcatc agaacagtac
     6661 actcaggtta tgggaagtga ttaccgcttg atccaggacg atatcccgtt ccagcaagcg
     6721 ccagttgtca tcttccagac ggcgaacttt gtcgaaacgc gttccaacat agaatgtttc
     6781 atcgcgctct ttttgtgccc gataaagcag ataatttacc cgcacagcaa atacgtttgg
     6841 gatgtcggtt tcgcttatct ggcagttgct gattaagtga cgggtgcgtg acggcggctc
     6901 ttctgcccag gccatgcccg tttccagacg ggcgattcgc cgctccagct ggtctttggt
     6961 gtcattaaaa atccaggttg tcggtggctg gacgcctttg cggcggtcgc gtgtttgcgc
     7021 gttaactgtg gtgcgcatgg tgtaacgaat ctcttcgtcg agctgcgcca gccagtcacg
     7081 aaatttccag tcgtccagta agctggcttc gtgaaagaga aactggctaa tgcggtgatg
     7141 taactctagt gaaacttgcg cactcatttc atcacctcct gctggtaagc ggcggtttta
     7201 tcgagcactt cctgccagct ttcgctactc agaagatcgg cccagcgttg gtacattcca
     7261 cgagcggcag tttctgaaaa gatatagtta gtaatgccag gaatgccatc gtcgcggcgc
     7321 ttttcctgac caagccccat ttccagacac agtttgctgt tgcgggcgcg gtggccttta
     7381 agcaatttct ggatttcaca ccagttctcc gagtcatcct gctcgagaaa accagcagga
     7441 ccaaaagcac gagtggcgct gttttcaaaa gcggctttaa cttcatcgga ggcggctttg
     7501 tcagtaatac agaacgccca cacttcaact tgatcagggc cgcgcggatg ccagacgcgg
     7561 agcgtggcag tgccgttgag ccatgaaagc gtggggaaaa tattgttatg acccgccagg
     7621 cgcagggcgc gaacttcacc taaacgttgt tctgcttcgg cataggtttc gcggtaatag
     7681 cttgaaactg cgccatcgac ccagacatta gcatccggtt tttcagtaaa gaagaaaccg
     7741 ctaccgtgac cgtcctgacc aaattgcagc gcatctttgg cggtttccca caccgggcgg
     7801 gcggtttgtc catcaccgag gcgcttatcg ctgccatcat ctttcgcccc taatacctga
     7861 acggcagaag catggctgaa cagagcatga tactggtcac tggcgaactg ctctgccggg
     7921 aatttccagt tacagttgat cacccacttt tgtacgccgc cgacaatttc ggtgccgcct
     7981 tcgcgacgat ccagcatgcc atccagatac caggcaatgt caccaaggta atcacgcagg
     8041 cccggtgcgc tggtatccca gttgccaaaa atcagccctt tataactctc cacacaagga
     8101 acttcgttta gcccccagtg ggatttacac aacccttgtg ggtaggcgcg aggttccagc
     8161 ggtacatcga tcaactcgcc gttaatgcca taagaccagc cgtgatacgg gcaggtaaag
     8221 gcgcgagtgt tgccgcaatc tgcataactc acacgcatgg cccggtggcg gcattggttg
     8281 agaaacgcct tgatgctgcc gtctttctga cgcactacga caaccgcatc ttctcccatg
     8341 taggtgttaa agaaatcacc gggttttggg atctggcttt cgtgggcgag aaatagccag
     8401 caacggccga aaatacgttc aagctccagt tggtaaatgt ccgggtcggt ataaatacgc
     8461 ggagtgaccc taccgttttg ggtgtcaatc agttggtaaa tgttcaaatc tgagggtgtg
     8521 gtcatatttt ttaatcctga aagataagat gtattgacct tctatttttg tgccataagg
     8581 atgttgctgg ttggttgcta atatgtgaaa tagttaattt ctaccagctg agacgttttt
     8641 tcgataacaa ggggaaggtg atggaactac ggcatttacg ctatttcgtc gcagtggcgc
     8701 aggcactgaa ctttacccgt gcggcggaaa aactgcatac ctcacagcct tcgttaagca
     8761 gccagatccg cgatcttgaa aactgtgtcg gtgttccgtt actggtgagg gataagcgca
     8821 aagtcgcgtt gacggcggcg ggagaatgtt ttctccagga cgcgctggca atcctcgaac
     8881 aagcggaaaa tgccaaatta cgggcgcgga aaattgttca ggaagacaga caattaacca
     8941 ttggctttgt gccatcggcg gaagtgaatt tattgccaaa agtattaccg atgtttcgtc
     9001 tcagacagcc agacaccttg attgagctgg tgagtttaat caccacgcaa caggaggaaa
     9061 aaattcgccg tggtgaactc gacgtcggct taatgcgcca tcctgtttat agcccggaga
     9121 ttgattatct ggagcttttt gacgaaccat tagtggtggt gttaccggtt gatcaccctt
     9181 tagcacatga aaaagagatc accgccgcgc agcttaatgg cgtgaatttc gtcagtaccg
     9241 atccggcgta ttccggttcg cttgcgccga tcgttaaagc gtggtttgcg caagaaaaca
     9301 gccagccaaa tatcgtccag gtggcaacga atattctggt gaccatgaat ctggtgggca
     9361 tggggctggg cgtcactttg atacccggtt atatgaataa ttttaatacc ggacaggttg
     9421 tttttcgtcc cattgccgga aatgtgcctt ccattgcttt actgatggcg tggaagaaag
     9481 gggagatgaa gccggcatta cgcgatttca tcgccattgt gcaggaacgt ttggcaagcg
     9541 taacggcata attacgcatt atttttgcaa catgcgtcgc ggatcgcagt tttttagtgc
     9601 ttgtgaactg gcgttacaga catcgggaag caggctcgaa tattcttatt tttcaacttg
     9661 caccaacatt cgattaaccc cagactaacg cctcctgacg ggagggactc atggttttgc
     9721 aatccacgcg ctggttggcg ctcggctatt tcacatactt ttttagttac ggcatttttc
     9781 tacctttctg gagcgtctgg cttaaaggga ttggtttaac gccagaaacc atcggcctgt
     9841 tattgggggc aggtctggtt gcccgttttc tcgggagttt gctcatcgcg ccccgcgtca
     9901 gtgatccttc tcgcctgatt tccgccttgc gcgtgctggc aatgatgaca cttctctttg
     9961 ctgtcgcctt ctgggcgggg gcgcacgtag cgtggctgat gctggtgatg attggcttta
    10021 acctcttttt ctcaccgctg gtaccgttga ccgatgcact ggcgaatacg tggcaaaagc
    10081 agttcccgct tgattacggc aaagtgcgac tgtggggctc ggtggcgttt gtcattggct
    10141 cggcgctgac gggcaaactg gtcactatgt ttgattatca ggtgatcctc gcgctgttga
    10201 cattgggcgt ggcatccatg ctgctcggct ttctcatccg tccgacgatt cagccacaag
    10261 gggcaagccg ccagcaggag agcaccggtt ggtcagcgtg gttggcgctg gttcgccaga
    10321 actggcgctt tctggcctgc gtttgtttat tgcagggggc acatgcggcc tattacggtt
    10381 ttagcgccat ttactggcag gcagttggct actcggcctc ggcggtgggc tatttgtggt
    10441 cgctgggcgt agtggcggaa gtcattatct ttgcgctgag taataaactt ttccgccgtt
    10501 gtagtgcacg cgatatgctg ttgatctcgg cgatttgcgg cgtagtgcgc tggggcatta
    10561 tgggagcaac tacggcgttg ccgtggttga tagtggtgca aattctgcat tgcggcacct
    10621 tcacggtctg ccacctggcc gccatgcgtt atattgctgc tcgccagggt agcgaagtca
    10681 tccgtttaca ggcggtttac tctgccgtcg cgatgggcgg cagtatcgct atcatgaccg
    10741 ttttcgccgg tttcctgtat caatatctgg gccacggcgt gttctgggtg atggcgctgg
    10801 tggcgcttcc ggcaatgttt ttgcgcccga aagttgttcc ctcatgctga ttcgagcatt
    10861 ttgcggatct gctcctgttg atgtgttgtc agtgtcaggt ccgtatagat cagcggtggt
    10921 gaaaagagcg gtaacggcat ggtataagga gcaataatca gtgccgcgcc tcgcggagcg
    10981 cctgtctgca aaaatgcctt taccgacata tgcttaatgt tgagcggcag taacgttagt
    11041 tcgcgtaact gctgctcaat ctgcgcttct cgctcgctat cattcccggt cagtagaata
    11101 atctgttttt catgcagatc gttttcctgc attagccagg caccgaaaat caccgcgacc
    11161 agaccacttt cctcatcaga aagatggacg ccgtattcac tttcaaatcc ggccagcgcc
    11221 gcgcgggtgg tgcgtaccag gcgtgggtac agtctggcga actcttccgg cagggtatta
    11281 tcaataccga tggcaaacaa actgcgattt aacgcctggg cgaggtgggt ataaagctga
    11341 tcgcataacc cctgttcatc ataaaaacga acatttccca gctcacgaaa atggtttacc
    11401 agacgtttga tagactgacg caattgtctg tctcgctgat gcgcatcacg caatggatcg
    11461 ggaacccgca acatggaaaa aagtaatgcc ataaacagtg gctcatcagg cggtacaggc
    11521 tggagcgccc gacgctgcca gtggcgtcca atttcctgcg ctacctgaaa ttcaaggcag
    11581 gattccgccc agcgacgttg gagcggatta aactgaggcg tcattccagc gtggtgctgc
    11641 agcaaacaat attgcagata cagacacagg aaatgaatat cgcgcgattc aaagcgtttt
    11701 tgcagccgtc gggagcagag attcactaac gcttgtagat tggtatcgtc gtaaaagtta
    11761 cgcgcaattc cgcgccgctt caattcactc ttcagggcgg gggtgaattg gctggtaata
    11821 aatgacggac acagacggaa accacgtcgt agccagtgaa ataaacacaa acgttggtta
    11881 agcactgtac cttcaacccg gtagctaccg tcataaccag tcgtcagtgt gagttgatga
    11941 tagcgcagga tctcccgtcc tgtttcgctg atatcaagac tggcaatatc atcatccaca
    12001 ccattaagct cgctgaaggt tgccatggtg gcggtcaacc ccggctgaaa gagcgtcagc
    12061 aagatctggc agcggcgctg gggagccgaa aggacagatg gtggagcaag cgtaggcatc
    12121 atctgtagat ctcccataag cataattgat aagaatagct aaagtttgct caaggaaatg
    12181 gcgaagggaa tgctaatcat cagaaatgtt ggcaagcgtc acagaatttt tctcatctta
    12241 aactgttctg accagtggat tagcatgtct taaggtggtt ttgtgaatga ttatcgtgct
    12301 agcgatcatg ggttagttct gcggattcaa cgcgctaagg ataaagtcca ccatctgttc
    12361 gcgataaaca tcgccgtgac gatcggaaaa agcatcaatg tgttcgccat ccgggattag
    12421 aatcaaccgt tttggctctt ttgccaggct atacaacttt tcgctgtgct gccatgggat
    12481 aacgtgatcg gctttaccgt gaatgagtaa aagcgggatc gggctgacgc tggcgatgta
    12541 attttcgcca ctgtaactct catcaagtaa gtagccactg ccggggatca tctggttggc
    12601 gatggttgca taagaggcaa atgtggagtc gaggatcacc gcacgtatgc cttcacgatc
    12661 accctgacca ataacagcaa gaatattcgc cccgccaata ctctgcccga acagcaccag
    12721 ccgttgtggg tttacatcac tgcgatggcg caccacattg atggcgcttt gcgtatcgtc
    12781 cagcaatccg gcctgggacg gcgtgccttt tgatttacca aacccgtgat aatcaaacat
    12841 aaaaacgttg aaattacgct cgggtaacca actgaccagc ggccagtggg cggacatatt
    12901 tccggcattg ccgtgagcat gaatgatggt tgcgatagcg ttgtcagcag ggcccgtcga
    12961 agaaggaata aaccagcctt gcagacgagt accgtcctta gccgtaaatt cgacggattc
    13021 cgccgaccag gggtcgggac cgtaaagttt atcgtcagga taatagaaga agttgatggc
    13081 aacgcgcggc actaaataaa cgcagaaggc aacaacaaag agaataaaca gaattttgag
    13141 aacgcgtttg ttcactggca gtgccataac ctttccttgc gacttcttta tcatccatta
    13201 atcggggaaa gatggtggga gagtgacgaa agaaagtaaa gcagtgagga agtatttcgg
    13261 aatagcgcct gaaaggcgag ggcgggtgag tgatatcacc cgcctgagtc attaacgctt
    13321 cagagcgtcg cttaactcgt cacgcatgtt cgccagcatg gctttaacaa cgcgcgggtt
    13381 accagcaacg atgttaccgg tcagcatata gttatgacca ccggtgaagt cgctgacgat
    13441 gccgcccgct tcacgaacca gcagctcgcc tgcagcgaag tcccacgggc gcagaccgat
    13501 ttcaaagaaa ccgtcaacac gacccgcagc gacgtaagcc agatccagcg ccgcagaacc
    13561 ggtacgacgg aagtctgcac attcgttgaa tagtttgccg acgatgttga tgtaggtagt
    13621 ggcgtactgt ttggctttga acgggaagcc ggtcgccaga atagtaccgt cgagatcgcg
    13681 agcggtgctg ccgcgcagac ggtagccgtt cagctgtgcg ccctgaccgc gagtggcggt
    13741 gaacagttcg ttacgcatag gatcgtatac cacagcaact tcggtgcggc ctttgatacg
    13801 aacagcgata gataccgcga agtgcggcag acgtttgata aagttggtag tgccatccag
    13861 tggatcgata acccattgaa catcctgatc agtaccttca agttcaccgc tttcttcggt
    13921 gatgatggtg tgctgtgggt aagatttacg aatcgtgtcg ataatcaccg cttcggcagc
    13981 tttatctacg ttggtcacga aatcgttact gcctttctgg ctcgcttcta cagcgtccgg
    14041 ggtttcatag tttttggcaa ttaaattacc cgccttgcgc gctgcgcgca cggcgatgtt
    14101 cagcatcgga tgcatcggtc tctctcactg gatgttaaag aacgggaaaa cggcgcagag
    14161 tatagcagcg agatcggcat ctgtcttcgt tttatgataa gatgcgtgaa taatctttag
    14221 atgaagaaag acaatgctgc aaaatattcg aattgtgctg gtggagacgt cacacaccgg
    14281 caatatgggt tctgttgccc gtgccatgaa aacaatggga ttaaccaatc tgtggctggt
    14341 taatccactg gtgaaacccg actcccaggc gattgccctg gcagcagggg ccagcgatgt
    14401 gattggtaat gctcacatcg tcgatacgct cgacgaagcg ttagccggtt gtagcctggt
    14461 ggtgggcacc agcgcacgtt cccgcacgct gccatggccg atgctcgacc cgcgcgaatg
    14521 cggcctgaaa agcgtcgctg aagcggcaaa taccccggtg gcgctggtgt ttggtcgcga
    14581 gcgcgtcggc ttgaccaatg aagagttgca gaaatgccat tatcatgtcg cgattgcggc
    14641 taacccggaa tacagctcgc tgaacctggc gatggcggtt caggttattg cctatgaagt
    14701 gcgcatggcc tggctggcaa ctcaggaaaa cggcgagcag gtcgagcatg aagagacgcc
    14761 gtatccgctg gtcgatgatc tggagcgttt ttatggtcat ctggaacaaa cgctgctggc
    14821 aaccggtttt atccgtgaaa accatccggg gcaggtgatg aataaattgc gccgtctgtt
    14881 tacccgtgcg cgcccggaaa gccaggagtt gaatatcctg cgcgggattc tggcttctat
    14941 tgagcagcag aataaaggta acaaggccga at
//
LOCUS       DBBGLQ010000108        14621 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.108, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000108 DBBGLQ010000000
VERSION     DBBGLQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.108"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..525
                     /gene="traF"
                     /locus_tag="ACIYSB_004278"
     CDS             <1..525
                     /gene="traF"
                     /locus_tag="ACIYSB_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HGC5431196.1"
                     /translation="LFNYNTLIKNYDSSAFKGNRYHNTQNGINADIGASMDLDDNWTL
                     GLVAQNLIPRSIETKEVHGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSD
                     SKRQFAAIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGT
                     DNNYGAVAQLQFTF"
     gene            665..835
                     /locus_tag="ACIYSB_004279"
     CDS             665..835
                     /locus_tag="ACIYSB_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431197.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNIVIANDICIRVALSPS
                     EVFQPFSKNTNQ"
     gene            884..1336
                     /locus_tag="ACIYSB_004280"
     CDS             884..1336
                     /locus_tag="ACIYSB_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HGC5431198.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            1333..2169
                     /locus_tag="ACIYSB_004281"
     CDS             1333..2169
                     /locus_tag="ACIYSB_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HGC5431199.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            2153..3301
                     /gene="traJ"
                     /locus_tag="ACIYSB_004282"
     CDS             2153..3301
                     /gene="traJ"
                     /locus_tag="ACIYSB_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HGC5431200.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSCIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFHDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            3298..3588
                     /locus_tag="ACIYSB_004283"
     CDS             3298..3588
                     /locus_tag="ACIYSB_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGC5431201.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            3652..7713
                     /locus_tag="ACIYSB_004284"
     CDS             3652..7713
                     /locus_tag="ACIYSB_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HGC5431202.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYKAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQARYARSYINGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDAGNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEEEKAQGLTDFNDLDASRGRDDFQHVMNAQLKHIGIPTPNSDTADHREA
                     VVIGNLIFTPVKDEKPQMSPENKQSTAPETVLATPDTPYDIHTEETENQAYEDDYAAY
                     ASLQSVTTETLSPDKPLENIASAADTGTENTSAATAGDQEDLNTDATGTDTAHNTNTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEEKASATT
                     ADNQSTQAEEHPRSEDTPETSLSDAAGIQHSTTTQSEEQVQVSMATADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAENS
                     SDITDDNRNAPAEELNADATVAVHNTGTAETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERPVSATETSTANEAAHPETPSLFSRLRRFLTGGKSRTAEPPVIQENKLTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEIIKNLEHKEFPDRTALYRV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKLRQEKGMAHDAIVTHRPTPELNRLSPEETTMAQPAPPVS
                     PKAETEQTAGSTAETPHPEAPVTAKAEAPRAGTPEQPAAPSSSAGNVAVSAQPQQPDT
                     PEEEPPGKLRPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGKGLEELVK
                     NHQKGDPVTLTLQARERSPSSPGKPEWVRNIWAMTPVSNGITVDNDLPHAGQHILEFP
                     MKTFSQVMSQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASEPQDQSMRLPLAGKAP
                     DELIPAVASADKDNRQLNLLLVHSADEHLQGVVRLNGTLYPALATPTTNNRQLVINAL
                     TDNGLQFAGYGEAVNHDENTHQRPAPQMMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            7730..8080
                     /locus_tag="ACIYSB_004285"
     CDS             7730..8080
                     /locus_tag="ACIYSB_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGC5431203.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKLLRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            8092..8787
                     /locus_tag="ACIYSB_004286"
     CDS             8092..8787
                     /locus_tag="ACIYSB_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HGC5431204.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHATDVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            8798..9772
                     /locus_tag="ACIYSB_004287"
     CDS             8798..9772
                     /locus_tag="ACIYSB_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HGC5431205.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQTPGSA
                     PAGGPPPATQLTGPLPPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPR
                     QGPASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            9776..11107
                     /gene="traO"
                     /locus_tag="ACIYSB_004288"
     CDS             9776..11107
                     /gene="traO"
                     /locus_tag="ACIYSB_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HGC5431206.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     KNQSRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGATGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     ERTGMSTVASDVNHRWFKHIVLPSILGGAGKGGSLYKDANTQILQGNYGSLTGRVGMP
                     DGRALAGVMAGGMAERGSELLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPTS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            11104..11817
                     /gene="traP"
                     /locus_tag="ACIYSB_004289"
     CDS             11104..11817
                     /gene="traP"
                     /locus_tag="ACIYSB_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HGC5431207.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     AAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPITETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            11814..12344
                     /gene="traQ"
                     /locus_tag="ACIYSB_004290"
     CDS             11814..12344
                     /gene="traQ"
                     /locus_tag="ACIYSB_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HGC5431208.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            12391..12789
                     /locus_tag="ACIYSB_004291"
     CDS             12391..12789
                     /locus_tag="ACIYSB_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HGC5431209.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVTIAQVIGLVLFIAGLLGLKQIGKKPGAGLACIL
                     SIVVGACLAVVPEMMSSGQKTLGLSPVSIS"
     gene            12846..13097
                     /locus_tag="ACIYSB_004292"
     CDS             12846..13097
                     /locus_tag="ACIYSB_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HGC5431210.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNIATHRPGPGTLAFPAPATGHGIQR"
     gene            13012..13830
                     /locus_tag="ACIYSB_004293"
     CDS             13012..13830
                     /locus_tag="ACIYSB_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HGC5431211.1"
                     /translation="MNKTSPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRQMLREKMDGVALILLPDSFPDKKYVM"
     gene            13887..14246
                     /locus_tag="ACIYSB_004294"
     CDS             13887..14246
                     /locus_tag="ACIYSB_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431212.1"
                     /translation="MDTLSAAYQFTADAFRHFIIRTLTDDIPPDGVEKEIARFILRHP
                     AFNGGWLLFLAAEIHEKSRTHDCITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            14246..>14621
                     /locus_tag="ACIYSB_004295"
     CDS             14246..>14621
                     /locus_tag="ACIYSB_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431213.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     RNERRKCIVVHINKITYPPVNSL"
BASE COUNT         3567 a         4350 c         3831 g         2873 t
ORIGIN      
        1 ctgtttaact acaatacgct gatcaaaaac tatgacagca gcgcattcaa aggtaaccgt
       61 taccacaaca cccaaaacgg tattaacgct gatatcggtg ccagcatgga cctggatgac
      121 aactggacgc tgggtctggt cgcacaaaac ctgatcccac gcagcattga gaccaaagag
      181 gtacatggtg tcactgaaac tttcaggatc cgcccgcagg tgactgccgg tgtctcctgg
      241 cacaacgaca tgttcactac agcactggat gttgacctga ccccggccag tggcttcaca
      301 tcagacagta aacgtcagtt tgctgccatt ggtgctgaat ttaatgcctg gaaatgggca
      361 cagttacgtg caggctaccg ccagaatctg gccagcaatg atggcagtgc gttcaccgcc
      421 gggctgggta tctcaccatt tgatgtcgtc catattgatg tggcgggtat agcaggcaca
      481 gacaacaact acggtgccgt tgcacaactg cagttcacat tctgatcatc tctgccccgg
      541 tactgaccgg ggtatccaga cctgcatcct cgcgttttcc gaccatcaaa cgacacagcc
      601 agacctgtag tgctgcctct gatctggctg ttatctgaca tatcattttc gcatgaggaa
      661 atgcatgttt aacaaaaata aaacgcatat cgaaaacaaa gcctttatgg tggaaccgtt
      721 ccgggatccg acagctaacg aatgtaatat cgtgatagca aacgatattt gtatccgggt
      781 agcactgtca ccctctgagg tgttccagcc attcagcaaa aacacgaacc agtaaatcct
      841 ccccacaacg ctttcttctt tcactgctca ggaaaaatac accatgaacc gtatgatacc
      901 tgcgggatgt ctcgccgtac tctgcctgaa cggctgccat ctccccccgg ataatacagc
      961 tccgaccaga aagtatggtg ctgcctctga tgtcgccgtc gcccggcatg cgcagtatca
     1021 gctatggggg cagggaattt actctgcagc ccttccggag cgtcagtccg gcggcagtct
     1081 gaaggctcac agcgaccgtg tttcctttga ctgggaaggc gatgccctgg aattgctcaa
     1141 cgaactggcc cgtctgcgcg gccagcagtt cagctacagc ggcgtccgtc tccccttacc
     1201 cgtctcactg cacgtccgcg acatgacctt tgctgatgtc cttcgtctca ctgaagccca
     1261 gactgcctgg cgggcaacac tctcgcagtc agccgggcgt ttacatctgt cttttatgac
     1321 gccggagaaa aaatgaaaac acacctgtgt ctcctgctgt ctgctctcct gcttacaggc
     1381 tgtgccggac agcatcaccg ttcttctgcc gtcatgacag aaaacgcacc gcccccggat
     1441 atccgtgcat ggctgacgcc ctccggcaac caggatgatg gtatttcaga tacccgtcgt
     1501 cagatgctca cggatgccgg cagaacgctg ggattccgtg gtggaaaagc acagcgctca
     1561 tgggaactga ttcaggcact gaatgcccgg gaaagtaccc tgaatgccct gtatgatttc
     1621 cggcctctca tcagcaagga aggctggctg cctcctgtta tcgatgaagc gcaggatgtt
     1681 gcacacatca gaccggacca gataagaaca gcctcccgtg tctggaccat cctccgcccg
     1741 gaacgctttg tcagtaatcc acctggatgg cgtgactggc tgctgaaagg actctcaacc
     1801 accgccacac caggcactga aggccgggtt gtgccggaag accgggcaca acgcagacta
     1861 tgggaaaacg ccttacgtca gggatggcag gaagggcgcc tcaacgcaga tctcacactg
     1921 gaagctaatc agaagcgcct gacccgtgat taccggggca tgatgcttta cgcccttctg
     1981 tggcgtcagg gcatgatcac acgtccagat gtcacagagc agatgcagac ggttaccggc
     2041 aacgggcaaa aactcatcac tggcgatcgc gtccgccgcc tgaaaacaca tgctgaattt
     2101 acgctgcaga aatcgcgctg gcgccctctt gtcagcaccg aaggagagaa aaatgatatc
     2161 accagatgac ttcggtacct tccccttctc ccgttttacg gcagaagagt ttcgccattt
     2221 ttttgcctgg tgtgcccgcc accgcgtcag tgatattgat ctgaccggcg gttcaccggt
     2281 atccgtcagt cgctttggac ggcgggttcg ctcatccgta aatccccttc cgtcctgcat
     2341 catgatggca ctgagtgatg agctgtttgg gcgggaggtt cttccccggg ttctgtcagg
     2401 tcatcccgtg gatcgtacca ttcaggtcag cggagatgcc tccggtcgct atggcctgca
     2461 acgaggtcag cgtgttcgcc tgcgctgcca cctgatacag ggaacatcgg caacagaaga
     2521 aaaagccatt tccgtcacca tgcgggtgat cccgtcagat attccggaca tcctcactat
     2581 gaatattgaa ccggatctgc tggatgccat gctcagaaaa accggcctgg gcttcatctg
     2641 cggagaaacc ggctccggga aatccacact ggcggcggca ctgtatcgcc acattctgaa
     2701 aaatttccac gatcgcaaaa tagtgaccta tgaagatccg gtggaataca ttctgggcgg
     2761 agagaatgac cttctgcctc cccatcaggc cgaaatcggg cgcgatgtgg aaagttttgc
     2821 tgcaggactt cgctctgccg ttcgccgtaa tccggacatc attggtatcg gtgaaatcag
     2881 ggattatgaa acagcagatg ccgcagtcca ggcgggtaat accggacact tttgtatcgg
     2941 caccatgcac acaaaatcac ccggagaaac ctttgcccgc ctgctgggat tgtttcctcc
     3001 tgaaatcagg gattcgatgg ctgcagccac gctttccctc gtacagttca ttctggtgca
     3061 ggtactggtc agaacaaatg acggcggcag gcaggctgtc cgggaataca tcgtcatgac
     3121 tgacgaactg cgcaatacgc tctccggaca atcccatgca acatggggac attacattga
     3181 cgaaatcatt cgtcaggaaa aacggcggat aagggatcag gttctgacca tgtatcagaa
     3241 cgaccgcatt gagaaatcag aagcagcgct cttcatcccg gccggagagt tcccccgatg
     3301 aaacgcgaag gcttctggcg ggatgtaaca ccaccgctca acatttatgg tgtccccctg
     3361 ccgtttacca tgatttacct gctgttgttt ccgtatccgt caaaaacgac attctgggtg
     3421 tgcagcggca ttctgctgtt ttttatgcta ctgggatatt ccggctggac agtccgcacc
     3481 ctctgtatac gtctgtacag cctgcttcag ggtaagcagg catcaggacg tccctggtgg
     3541 taccgacacc gcacagaatt accccgtgaa tggcacggac cagaatagcc ctcttttgtc
     3601 catcatcccg gcgtatcaca acgccctcat ctttaatccc cggagaacga catgaagtcc
     3661 agatatcgaa cctggcttgc tgtcccgcct gaggagacag aagccgtaaa aaatgcagtt
     3721 cccccgttaa atggccgcaa agctgtcgca tgggatccag aaaaaaagct ctggtatgcc
     3781 cgggcgggta ccgaactttc actgctggag cgctggttac cccggccaca ggaactgtcg
     3841 atggatgccg gcgatccggt cactgaattt gctcaggtcc tggaaaacgc cgggctggtt
     3901 attcagggat tgccacagat ggatggcgcc atacacagag tcgccacccg ggatgacaaa
     3961 aaaggggcca aaagtggcgc atacaaagcc tatcttgatg ggcgccccgc tggctggtac
     4021 cgcgactacc ggagcgccga tgacagtccc acaaactggg tattttccgg cggagaacaa
     4081 cacgatcctc tggccagact gcatctgcgg gcattcgctc agcaacagcg cgatgataac
     4141 gcacgtaaac tgcagcaaca gtacaacaaa caggcccggt atgcccgctc ctatattaac
     4201 gggttgccac aggcaacagc acatgaatac ctgacgcgca agggcatccg ggccgcgcct
     4261 ggcgtgcggc tgaacaataa aaatgagctg gttattccct tcagcaacgg ccggggggaa
     4321 atacgttcgt accagcgtat tccggtcacc ggcgggaagg atgcccgcat tctgaaagac
     4381 tccgagaaaa ccggcaactg gttcaccttc ggaaccccgg aaaatggccg tcccctgtta
     4441 tttgcggaag gttacgctac tgccgcctcc ctgcacgaag ccaccggcct tccggttctg
     4501 atgacagttg atgccggcaa tatgattgcc gtggcagaaa atgcccgcca aatctggact
     4561 gacagcccct ttgtcttctg tgccgacaac gatcatcaac gggaaattaa caagggggtt
     4621 tttagtgcaa caaaagccgc agaagtcacg aatggcgaag tcattattcc ggcctttacg
     4681 gaggaggaaa aagcacaggg actgaccgat ttcaatgacc tggacgccag ccggggacgg
     4741 gacgactttc agcatgtcat gaacgcacag ttaaaacata tcggtatccc cacacccaac
     4801 agtgacactg ccgaccaccg tgaggctgtc gtgatcggca acctgatatt cacaccggta
     4861 aaagatgaga agccacaaat gtcccctgaa aacaaacaga gcacagcccc ggaaacggtg
     4921 ctggcgacac cggatacgcc ttatgacatc cacactgaag agacagaaaa ccaggcatat
     4981 gaagatgact atgccgccta tgcatccctg cagtcagtaa ctacggagac gttatccccg
     5041 gataaaccac tggagaatat tgcgtctgcc gctgataccg gcacagagaa tacctctgcg
     5101 gctaccgctg gcgaccagga agacctcaat acagacgcca caggtacaga caccgctcac
     5161 aacaccaaca cagcagaaac aggaacgctt cctgaacctg ccgggatgca gtccgtgcag
     5221 caggcaacca ctgaaccgac cgcagaagat aaaccacacg agaacgttgt ttccgctgcc
     5281 gataccaggg aagaaaatgc ctctgtggct accgctgata ccggtgaaga aaaagcctct
     5341 gccaccaccg ctgacaacca gagcacgcag gcagaagaac atccccggag tgaagacaca
     5401 ccggaaacct ccctctccga cgcggccggg atacagcact ccaccacaac gcagtctgaa
     5461 gaacaagtgc aggtctccat ggccaccgct gacgactgga gcgcatttgc ggaagatctc
     5521 aacgctgaca ctccgggggc ccccactgtt cacaacaccg acaacgcgga gacaaacaca
     5581 gtttctgaac cggccgggat gcggccagtt cagcaggtga ccactgaact gacggcagaa
     5641 gataaagcac aggaaaagag cgttgcgtct gccgctgaga ccggcgcaga gaactcctct
     5701 gacatcaccg atgacaacag gaacgcaccc gctgaggagc tcaatgccga tgccacggtt
     5761 gctgttcaca acaccggcac agcagaaaca gcctcacctt ctgaacctgc agaaactcag
     5821 ccagtccggc aggccaccgc tgaaccgacg gctgaagata aactgcagga agagcgtcct
     5881 gtgtccgcca ctgagaccag cacagcgaat gaagcagccc accctgaaac accatcgctt
     5941 ttcagccgcc tgcgccgttt tctgacaggt ggaaaatcac gtaccgccga acctcccgtt
     6001 attcaggaga acaagcttac ggaggccgaa actgcgacag aggaggtcgc accaccgtca
     6061 ccaccggaaa ccagcccgga agacacatcg tcagcagaac cggacacgat tatctttgcg
     6121 ccccgccgtc ccgacagtcc gcagcggcac aacctggacg agattattaa aaatcttgag
     6181 cacaaagagt ttcccgacag gacagcgctc taccgtgttg acggcgaacc agccttcctg
     6241 gacagactct attgtctcga aatggtggat ggtgccagcg cggatgacaa aaaggttctg
     6301 gctgcccttg ctgtggccac caacttctat ggcggcgtta tcgagctgac cggcagtgat
     6361 gcctttaaac aaaaagccat gcagctgatt gtcgaatatg acatcaaggt tcgcatgaaa
     6421 ctcccggccc agagggctgc gctggaaaaa ctccgccagg agaaagggat ggcgcatgat
     6481 gccatcgtca cccacagacc cacgccggaa ctcaaccgcc tgtcaccgga agagacaaca
     6541 atggcacaac cggccccccc tgtgtcaccg aaagcagaaa cagagcaaac tgcaggcagt
     6601 acggctgaaa caccgcatcc ggaagcgccg gtcacggcaa aagctgaagc cccccgggcc
     6661 ggcacaccgg aacaaccagc cgccccgtca tcgtcagcag gaaacgtcgc tgtcagtgca
     6721 cagccacagc aacccgacac accggaggaa gagccccccg gcaaactcag acccggtgaa
     6781 agtgtgactg ccgtcctgac aaactacgga gaaaaagagt atgcgcccgg aaaagggcgc
     6841 tgcttttttg tggagctcac caaccgcagt ggcaaacggg aatactgggg aaaaggcctg
     6901 gaagaactgg tcaaaaacca ccagaaaggc gaccccgtca cgctgacatt gcaggcccgg
     6961 gagagatcac cttcatcacc gggcaaaccg gaatgggtgc gaaatatctg ggccatgact
     7021 cctgtcagta acggcattac cgtcgataat gaccttcctc atgctggtca gcatatcctg
     7081 gaatttccga tgaaaacatt cagtcaggta atgtcacaaa tgcagcaaca ctggccacag
     7141 ataatgcagg aagttcgttc ccccaaaaat cctcccaata atctgtacat tggtgaggac
     7201 agacaaccgg catcagaacc tcaggatcaa tcaatgcgac tccccctcgc tggtaaagcc
     7261 cccgatgaac tgattcctgc cgtcgcctcc gccgataagg acaatcgtca gttgaatctt
     7321 ctgctggtcc acagcgcaga tgagcatctg cagggagtgg tccgtctgaa cggcaccctc
     7381 tacccggcac tggccacccc gacaacaaac aaccgtcagc tggtcatcaa tgcactgacg
     7441 gataacggtc tgcagtttgc cggctacggt gaagctgtca accacgatga gaatactcat
     7501 cagcgtccgg ccccacaaat gatgcagttt cacctgaagc aacaggactc cccgttgttt
     7561 gccgccatcc acaaaccgga agagcagccg gacaagcttt tccgctctct gggttttgag
     7621 cagacctgga aagagtggtc tgacagccaa aaggcggagg acaggcagga aaaaactctg
     7681 caacaggcac aatctcacag ccccggccga tgagccggta aggacgacca tgaacatgaa
     7741 aaaaacgctg ctggtcatac tgaccggcat caccctggca tcaccggtgg caggtgccgc
     7801 cacctgccgg gcgggggccg ctgccgaagc cggcagcaga gccgcctggg agcgggcgcg
     7861 cacagccagc caggcatggt cacagcggga gaatgacgtc gcctcttccc tgcagtcctg
     7921 cctgtcccgc atcaaggacg tcagcatcac tcttccgcag ttccccagcc tggacaaact
     7981 cctccgcgac ctggaaaacc aggtctgtga tgccgtcgtg gatcgggtga atgaacatct
     8041 gccggggaat atcgacccct ggaaagacta taacctctga acaggagcac catgagcgaa
     8101 gcacaaccca cctcccctgt ctcaccggaa gcggataaca gcggagatat ttataacccc
     8161 gtgctcagaa ccatcgcaca gtcagaacaa cgggccgcac tggttcccgg actggtcagg
     8221 gccttgctgt gtagcagcgc agctctcgtc atcagtctga ccggcaatat catacagtac
     8281 tggcatgcca ccgacgtgac gcgcgaatac tttgccaccg acaatggccg gctggtccgc
     8341 ctggccccca ccagtcagcc agcctggagt cagagcgacg tcatgacgtt tggcagcatg
     8401 acgctgagcg aagcttttaa tctggatttt gtccactaca ggaaacaaat cacgacactg
     8461 gctccacgct tttccgaagc cggttttgcc ggctatgtga atgctcttca ggcctccaac
     8521 attctggaca ccattaaaaa ggagaaattt aacctcacag ccaccatcgg ggccggcacg
     8581 ctggtgaatc agggccagat ggaaaacggc atctggttct ggaaattcca gtatccggtc
     8641 aggatgcgtc tggtcggtca gaccagcacc cgccccgagc aggccttcac ctttgaaatc
     8701 actgtccggc gcgtggaccc acgaatcaaa cccggcggca tggaaatcag ccagatggtt
     8761 tcccgtaacg ccgcagcagg aatctgagac tcatatcatg caaatacatc atcttcttgg
     8821 cgcactgctt attgtgtgcg gcactgtcac ggctgcagat aacccgggct ggcagaacgc
     8881 acgaacccca caggctggcg gaaagacagg acagactccc gggagcgccc ccgcaggcgg
     8941 ccccccgcca gccacacaac tcacaggccc cctgccgcca cctgcctctc ctcttgttca
     9001 ggaagccgca tcactggatt cccccctgaa tgcaggtgaa attcgggaac tgcggggacg
     9061 aatggccgat aacgaacggg ccatcagcgc acctgtcacc agtgtggtgc cccgtatcag
     9121 ctccctcacg gtaaatctgt cacccggggc cagtctgccg cttgtccgaa ccgcactgca
     9181 taacgtcagc gtggtaacgt tcacggacat caacggctcc ccgtggcctc agtcggatcc
     9241 gccttataac gcggcacccg gtcactttga tgtgcagttc aacggcaata tggtctccgt
     9301 cacccccctg cgcgcctggg ctgcagggaa tgtaacggtc tacctcaaag gactgtctgt
     9361 accggtcatt ctgaatatca ccagtggtga aaccgacacc aataccctga gtcaggaaat
     9421 cgacagccgt ctggatctgc gcattccccg tcagggacct gccagtcctg tggtagccgt
     9481 accatcagag aagattgccc tgcatgatgc caccctgcag gcctttctgg acggtgttcc
     9541 tcccgatgat cccaccgtgc gtcggctgaa attctccgga aatgttccgg acaccaccat
     9601 ctggcagcac ggcgatgatc tgctcgtgcg atcgcgggcc atgctgcggg atgaatttga
     9661 acagacgctg gcatctgctg acggtacgcg tctgtggaaa ctgccggtga ctcccttact
     9721 caccttctcc gtgaatggtc agtccattca cgtcacccct gaactggagt aaatcatgag
     9781 cgcagaacag gacacagccg caaagtccgg caaaaaactg tcagccctgc tggttctcgg
     9841 gggcgtgatt atcatcgggg gcggatacct gctgctcacc tggctttccc ccgaaaacag
     9901 cgcatcgctc tcgtcagtca atatcaacag cacgacaaca ggcagcgggc ggaccgtggt
     9961 ggagtcacag cactaccggg atttactgcg tgctgacaat gagcgcgggg ccgcagaagc
    10021 attacgtaat aaccagacgt tcatcgccag tctgcccagg ggactggata ctgatcacgg
    10081 aaagaatcaa agtagggata ctcagcctgc tgaccaggcc gagggttctg gtcaaccatc
    10141 aacgccccct caggcagatc aaacagcccg ggaaaaacag cgggaagcgt tacaaaaact
    10201 gctggcccgc atcaacacac agcatcctga cgcccgcccc ccggtagtgg cggctgcaat
    10261 gtggggtgcc actggtgctc ccgggataag ctcacacgcc tcaccacccg gtaccggcac
    10321 atcaccacaa ccacgtctgc aggaggccag tctggcaacc ccggcagctc gtacagggat
    10381 acagctgata ccggcactga cccgcgtacc tgccttcatt gatacggaag tggactctga
    10441 caatgctgtc tcccgcgtgg tggcctccgt acctgccggc ccatgggccg gggccgtcct
    10501 ctacagccag gacgccagac tcgccggtaa gggaatggat atccactttg accggatgat
    10561 gtggcggggc atgtttctga aggtcaatgc atatgccctg aatgaacgaa caggaatgtc
    10621 aaccgtggcc agcgacgtga accaccgctg gtttaagcac attgttctgc cgtccatact
    10681 tggcggtgcg ggaaagggag ggagtcttta caaagacgcc aacacacaga tccttcaggg
    10741 aaactacggc agtcttaccg gcagggtggg aatgcctgat ggcagggcgc tggccggcgt
    10801 catggcaggc ggcatggccg aacggggcag cgagctgctg acccgccagg cagagagcga
    10861 accttaccgt caggtgacag tatcacgggg cgaggtcgtc tcaattctgt ttgttgagcc
    10921 ggtcatgagc aacgatatca gtcccacctc ctccggcact gccccttctg tgccaccgcc
    10981 ctccggacgc ccgctcaccc aggaagaagc agagcgtcag gcgtctgaca ggatccaggc
    11041 agctgtcatg cgtaaacaga acgaaatgcg gcaacgttac ggaatacaac aggaaagtca
    11101 tccatgaaaa acgaacatga agatcccggc agttttggcg aaacaacgac accggcatcc
    11161 ggcaaatcct tcctgaaacg ccagtatatg ggatacagcc tgccagtctg ggccggctgt
    11221 gtcctcatca tcctggccgc agtctggtac aagtttctgc ggacaccctc agagccccct
    11281 gcagatgcag gcttcgctgt cacaccgccc gcagccgaaa gctgggcaga gcccatgccc
    11341 ataacggaga ccgggagcgg aaacaccatg tccgcgcctc ctgtcacccc acaggcagac
    11401 atgcagcagc ttgcccgtga cctgaaagca gagctggaca ggcgggatga ggaaatccga
    11461 cgtaccctga ccatgctgaa agagagcatc acaacccttg gcgacaccat acggaaagat
    11521 gaggcctggg cacaggaaac ccgtcagttg cttgatgaaa cccgtaagca ggtgcatgcc
    11581 ctgcagatgc agggaccact gcaaatcccg accgggaata acggccagtc aacgaaaacc
    11641 ccgccttccc gcctgcaggg catgcaaatc atgtcggtgg aaaaaggaat ggcctggatc
    11701 cgctggcagg gtagcgcctg ggcagtacgg gaaggtgaca ggctggggaa tatcaccatc
    11761 acccggattg aaccgtccag ccgctcagtc atcacctctg ccggtacatt acgatgatga
    11821 gcattgatgc cctgacagcc atagagaact ttgccagcgg tatattcagt gcaggcatgg
    11881 agtttctctt cacctgggga gagctgctgg ggattatcgg cctgattggt catctgatgc
    11941 gcgcccgggc agaagggcgt cacagcatgg gaccgggaaa atttatcgcc ggtatcgtca
    12001 tctgcggcat gctggtcgcc ctgccatcct tcatcaatgc cgggggtacc cagatgggat
    12061 tcagggcaga ttccttcggc cctatcgctt atgtacaacc aaccaccttt ggtgccgctg
    12121 ccggtgccgc caatgccatg ctctcactgg taaaactggc cggagtggga tttgccatga
    12181 acggcatttc catctggcgt aaatccctgc tggacgggca taccgccctg agcagcagtg
    12241 agtccatcag caaaggaaac gtcaagtttg ttgccggcgt tctgctggtt tttatcgatc
    12301 gcgtcatcga tgcctctctg gcgagtcttg gtctcgcctt ctgatccaca ttcattttca
    12361 gtaattcagt ctgttagtta aggagactat atgtcacgat tccgtgccat ggcggcgcgt
    12421 ctgtgcgctg catttattta tgttcagtta tgcctggtgg cctgccgtga ccggctgata
    12481 cgcctgttac ttctgcttcc cgtgatactg ctgccccaca cagcgatggc aaacacccta
    12541 ttcgatatga tcaaaaccgt tggcgatggt gcaaaaggct ccagcagttc atttgtgacc
    12601 atcgctcagg ttattggcct ggtgctcttt attgccggcc tgcttggcct caaacaaatc
    12661 ggtaaaaaac ccggagcagg gctggcctgc atactgtcca ttgtggtcgg tgcctgtctt
    12721 gccgtcgtac cggaaatgat gagcagcgga cagaaaacgc tcggtctttc tccggtctcc
    12781 atcagttaat ccacaacggc accggtttcc cggtgccgtc ttcacacaat gaagaggacc
    12841 agaccatgtc ctgtgagctg ccccctttca ccctgctcag aacagagcac ggcaacgtga
    12901 ttgcccgcga caaccgggat aatcaggagt ttgtgtttgc agagtgttat tccaccgcca
    12961 tcgcagcact gattctggct gccgtcaccc tgtatacaga gagaaaaacg tatgaacaaa
    13021 acatcgccac tcatcgcccc ggcccgggaa cgctggcttt tcccgctccc gcaaccggac
    13081 acggtattca gcggtgaatc cctgctgcca ccacccgtgc tctcaacacc cggacgctgt
    13141 ccctgctgtc gcaggaccgt cactcaccgc ttcattctgg aggattcatg gccattacag
    13201 cagatggctg acacctgccg ggatacggtc gtcctgctgg aaaaaaacct cacccgggtg
    13261 atgcgcctga aaaaacatcc ggtaccggaa aacgctgacg aaaaaaagaa acacacccga
    13321 accctgcagg atgcggagcg gtccctggca caggcccgcc tgtcggccag gcgactggcc
    13381 ctgcgtcatg tggaaaagtc acaaatcgtc acaacagatg cactcagcga aaatgaaagt
    13441 gaactgctgc agccggaagg cccgcccttt catctctgtg ccttctgcca cgcctggcac
    13501 tgcctgaacg gctacgctgc cgcacagggg gtgatggtct ggctccctga tttgcatccg
    13561 gcctcggtag ttgctctcaa tgcacgggca ctgaaggaaa ttttcagcga tgagcgtaag
    13621 cgagtcagac agggacgggc cgtactgaat gccctggtac agaaccgcct ggccgttgag
    13681 gagaaatttc gcacctggcg tccggcggat tttgctgatg cactgcgccg ctggccgccg
    13741 gcacagcgtc aaatgttgcg ggagaaaatg gacggtgtgg cgcttatcct gctgccggat
    13801 tcgtttcctg acaaaaagta cgtcatgtga cacacaccgg tgccggtgac gttctgccgg
    13861 ccctctcaac ttacagagga acaaaaatgg ataccctctc tgccgcatat cagttcaccg
    13921 ctgatgcatt ccggcacttt attatccgga cactgacgga cgatattccg cctgatggag
    13981 ttgaaaaaga gattgcccgg ttcattctcc gccacccggc atttaacggc ggctggctgc
    14041 tctttctggc tgcagaaatt catgaaaaat cccgtaccca tgactgcatc accgtcacag
    14101 tgagcgtcga taactatctc tcatggatgg atattgccgt gtccggtctc ccggaattac
    14161 cggatatgcc cttctgtgat tttttcattc gccgccagca gtttatttat ccgggtgtac
    14221 ccccgacaaa atcaggagaa gaatgatgaa aattcaccgc atatccccgg agacgcttat
    14281 aacactcatc ctcgcacacc tggccggaaa agctgacagt accgccaaag aagagcaccg
    14341 gctactccgt cggttccttc gggatgatga cggcaggctg gccggcatac tgctgaatat
    14401 tgccggaata cttcagttca acagggaatt aagcgcccgg cataattatc cggccacacc
    14461 cctgactgaa ttttcattaa ggaaacgggg aaaacaactg catctctgtc tctgcagtct
    14521 ccgtttcttt tatatccccc ctgtatttat acgaaatgaa agaagaaaat gtattgttgt
    14581 acatattaat aaaatcactt atccgccagt aaattcacta c
//
LOCUS       DBBGLQ010000109        14601 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.109, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000109 DBBGLQ010000000
VERSION     DBBGLQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.109"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            157..3201
                     /locus_tag="ACIYSB_004296"
     CDS             157..3201
                     /locus_tag="ACIYSB_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGC5431214.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDSGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            3201..3818
                     /locus_tag="ACIYSB_004297"
     CDS             3201..3818
                     /locus_tag="ACIYSB_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HGC5431215.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHS"
     gene            3779..4984
                     /gene="traW"
                     /locus_tag="ACIYSB_004298"
     CDS             3779..4984
                     /gene="traW"
                     /locus_tag="ACIYSB_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HGC5431216.1"
                     /translation="MQRQSATTLLALLIGCAVIPATHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            4981..5550
                     /gene="traX"
                     /locus_tag="ACIYSB_004299"
     CDS             4981..5550
                     /gene="traX"
                     /locus_tag="ACIYSB_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HGC5431217.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPEIPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            5623..7788
                     /locus_tag="ACIYSB_004300"
     CDS             5623..7788
                     /locus_tag="ACIYSB_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HGC5431218.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNTGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPADNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVRGAYQ
                     AQLQNSAYTPALGTATSASEYHASKTQDPQSAILKLIGFNLTRMTNWTATYLSGTGTT
                     TNQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREEKLKNINGDDKDGIKQ"
     gene            7876..8529
                     /gene="excA"
                     /locus_tag="ACIYSB_004301"
     CDS             7876..8529
                     /gene="excA"
                     /locus_tag="ACIYSB_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HGC5431219.1"
                     /translation="MNTTKNRYPSWWAYYYLVRNAYFIFGVPFLLLCSLGFTGMMLTE
                     PYIETDIQAYLYTYGSWLLLILPSLWLYMKTRAKKKKVQQVVQQIKESSPFAPTSDYE
                     QLSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVS
                     LPMIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            8685..8966
                     /locus_tag="ACIYSB_004302"
     CDS             8685..8966
                     /locus_tag="ACIYSB_004302"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGC5431220.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIISAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNILYTK"
     gene            8972..9460
                     /locus_tag="ACIYSB_004303"
     CDS             8972..9460
                     /locus_tag="ACIYSB_004303"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGC5431221.1"
                     /translation="MFQLKPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AIDNDHRIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GTLLADALYRAYTSEIAAYALIVDAKDDTAVSFYKHYGFMTLPESGRTLFMPLASLSA
                     IE"
     gene            complement(9547..9699)
                     /locus_tag="ACIYSB_004304"
     CDS             complement(9547..9699)
                     /locus_tag="ACIYSB_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGC5431222.1"
                     /translation="MPQRTFLIMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            10028..10270
                     /locus_tag="ACIYSB_004305"
     CDS             10028..10270
                     /locus_tag="ACIYSB_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431223.1"
                     /translation="MKDEIQSMCLTPDIVKQFNTLLNTHCQSSEEALGMLSALCFELM
                     MKCGVQSINSLMVNGMGISMQLQEIDIAERPGKNIH"
     gene            10366..10557
                     /locus_tag="ACIYSB_004306"
     CDS             10366..10557
                     /locus_tag="ACIYSB_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431224.1"
                     /translation="MKKIKTHTGLLASKDKNRRVSLYETPTAWCIRGQECYSKSTGRR
                     CGSHDSLSRLRLDSIKPVE"
     gene            10569..10937
                     /locus_tag="ACIYSB_004307"
     CDS             10569..10937
                     /locus_tag="ACIYSB_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431225.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPGQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            10941..11156
                     /locus_tag="ACIYSB_004308"
     CDS             10941..11156
                     /locus_tag="ACIYSB_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431226.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(11354..11977)
                     /locus_tag="ACIYSB_004309"
     CDS             complement(11354..11977)
                     /locus_tag="ACIYSB_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGC5431227.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRVDSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYRL"
     gene            12137..12604
                     /locus_tag="ACIYSB_004310"
     CDS             12137..12604
                     /locus_tag="ACIYSB_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGC5431228.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            12755..12931
                     /locus_tag="ACIYSB_004311"
     CDS             12755..12931
                     /locus_tag="ACIYSB_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431229.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFGSR
                     EQAQEVQEGFRFLT"
     gene            complement(12994..13077)
                     /gene="dqlB"
                     /locus_tag="ACIYSB_004312"
     CDS             complement(12994..13077)
                     /gene="dqlB"
                     /locus_tag="ACIYSB_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HGC5431230.1"
                     /translation="MINLLIIVLRAVVAVANAVIALRELVD"
     gene            13388..>14601
                     /locus_tag="ACIYSB_004313"
     CDS             13388..>14601
                     /locus_tag="ACIYSB_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HGC5431231.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTG"
BASE COUNT         3620 a         4080 c         3636 g         3265 t
ORIGIN      
        1 tacattttta ttatttactg taatcacaca gaaatacgca cctgcccctc aacagattat
       61 ttattttctc tgctgttttt tcatgccaca tcccctgctg tggcatcaaa aaatatcaga
      121 ccgtgatgat tcatccttac cggagtgctc catgccatga atatcaaccg cattattttt
      181 gccattgaag actgtatcag taccctgtca cggtattccg tttccgcatt cttccccgat
      241 tactgtgatc tgcacaccgt catcggtctg gaccagcagg accgcgaacg ccgcccctgg
      301 ctgaaagccc cctacattgc caccacaaaa caggggtatt acctttccgt ctttgaaatc
      361 agcggtgccc tgaaagaaat ggatgaaaac ccggacctga cagcccccgg cacactggaa
      421 tcactgatta cagcgctggc agagcgaatg aacactgcct ataaaaacat cggtcacaaa
      481 atcagttttg ttttcgaacg cgatccggag cgtggaaggg atgaaatcga agccatgctc
      541 ttaccccagc gaaaatccat cgcacgcacg gggatcctgc ttcaggacgt tctggatgaa
      601 aaagtcacca cgctcacccc ctggcttgtc cgggaacgct gctggctgac cgtctggagc
      661 tctgaagaac tgctgagccg gaccgatcgc aaggaccacc agacccgtat caggaaactc
      721 gctgaacacg ctccgccagc ccgatttgcg caggatccat ggcgctggac actttccgca
      781 ctcaaaatac ggcatgacgc ctttctggac acgctggagc aggccctgac acatgattct
      841 gatggtctgc tggttcgcct gatggatatt catgaagtgg gacgggaaat ccgccgtcaa
      901 ctggagcgca acagcacacc tgcagtatgg cagcctcatc tgcctgaaga tgcccgtcct
      961 gccggctggc gacagggcga ggatacctct gtattccacg caccgtcgct gaatctgcag
     1021 ttattcacca cacagcctga aacacacggc agcctgataa aggccggtga cctctggcac
     1081 ggtatggtcg ccattaccct gccaccccag aatctgcgga cattcaacca gctggtcagg
     1141 gatgtgcccc gcgcaatccc ctggcgtatc cggatggatc tgatgcccgg cggcatgaag
     1201 gcgcttggag tcaagaaaac cctgctaacc tacagcagct ttatttcccc tctccggccc
     1261 atgtatgact cggtgatgat gctgaatgac atcaacaaac atgatccggt ctgtatcatg
     1321 accattgttg ccaccacatg gggaaacagc cgggaagtct gtacccgtaa ccaggctctc
     1381 ctgaaatctg cgctggaagg ctggggggta tgcggcacca ccaccacctt cggtgatccg
     1441 agacgtgcct gggtgaacac ggttctggca gcctcccaca gctccggccc gatccccctt
     1501 tacccgcccc tttcacacgc cctttccctg tttcccctga acagggcagg ctctgtctgg
     1561 cggggacagg gaaatctgat gatgcacacc gaggacggct ctgcatggga ggtcgcactg
     1621 gcatcgtcac aacagaacaa gcacacggaa ctgacgcccg gcgcacccgg tctgggcaaa
     1681 tcggtactca tcaacgcact gtcggagatc cagatagcct cagcccagaa gaacctgcct
     1741 ttcattgcct atatcgacaa gggattcagt gcacagggac tggtacagct tatccgtgac
     1801 agcctgccgg agcagagaaa ggacgaagcc gtggggatca ttctgagcaa tgatccggaa
     1861 cacacccgta acctgtttga tgtgatgtac ggtgctaaaa aacccgccac cccggaaaag
     1921 aacttcatgg tatctgtact ctgcgcactg tgcgtggata ccggcaccgg tcagccctgt
     1981 aatcccggag acacccggca aatcataagc aacctggtgg atctggcctt cagggagtat
     2041 ggcgaaaaca accccagact gtaccgtgca ggaacagagc cccttgtgga tctcgccctg
     2101 gaagagagtg gcatcgcaga acaacatgac tctggatggt ggaatgctgc cacctggttt
     2161 gaaatcaggg acatgctgca tatggctggc aatatccccg cagcccagcg ggcacactac
     2221 caggccatgc ccctgctggc tgaaatgtcc gccctgctgg gacagccatc catccgggat
     2281 gtgttcggca cagttcagcg cgataactca gcagaattgt tactggacta catccgccgt
     2341 gccctcgatc agggacacag tgactacccg atgatgtccg gctgtacccg gtttatgctc
     2401 agccccgaca cccgcgttgt ggccgttgat ctgaacaatg tggccggtga caagacacct
     2461 gccgggcgtc tgcgcaccgg catcatgtac ctgctggcag gacaaatcgc cggcggggat
     2521 tttgtcctgc cgcagtatca ggaggaaata cggaaaaatc tcgacccacg ctaccatgag
     2581 gtgattttcc gccgcattga acagcttgat caggaggtca agaccaaagt ctatgacgaa
     2641 ctgcacaatg caaaagggat caacttcatc tgggaagccc tggacaccca ggagctggag
     2701 cagcgtaagt tcgggatacg cacagtgctg agcacccagc tactgcagga ctatccaccg
     2761 gcagttctca aatccgccaa caccctctgg ttgctgcgtt accgccctga cgaaattccg
     2821 tttctgcggg ataattttgg tgtccctgaa gtcaccctgc ggcgctttct gaaaatgccg
     2881 gaaggggctg caccggatgg cagcggtgtc ccggtactgg ctgtgttccg tgtaaaaaac
     2941 ggtacgctgg cccggatcct gaaattcact ctcggtccac tggagctgtg ggctctgaat
     3001 tcctcaccca aagacagcgc cctgagacgt gcgctgacgc aggagctcgg gtcactgcgg
     3061 gcacgccaga tcctggcaga aaactttccc cgtggttcag ccacctcact gatagaacac
     3121 cgcgcccgta cacatgacag tgaaaacgtc atccatgatc tggctgcgga actcatccgt
     3181 aagcagggat atcacctctg atgtacagca tacacatcac tccccgctgc cggcgtcctg
     3241 cacggggaca ggtcttcctg aaaacgctgc gcgtggacgg tgacagtttc agcttcaccc
     3301 tgccgtccgc ctgcttcaca ctgcgaccgt cccccaatcc ccgggtcatt tttgccagtg
     3361 acagacgggc tgacggggag atgagtggta ttcgcctggc cctgcccgga gagacaggca
     3421 cgctgattac aacggccacc tggcaggtta acgtgccgga gcgaccgcct tttgatatac
     3481 agcatgtcat tcactggctt ctgcccgcac cactcactgc gctgatgtct gacaacacct
     3541 ggctgtggcc ggatgtcccc ggcacaccgg cactcacagc cggagactgg ccggtcatgg
     3601 aaagcgacac cccgacactg aaacgcgtga agcgtcgtac cttcacgctc atcgataacc
     3661 gtaaccgtca gggatggatc acgcgccgct tccagtgttt cccgctggct ccgctgccgg
     3721 aatcactcag tcatcgcgta ccagacatcc tgtatccccc gaaaacggag aaaaaagaat
     3781 gcaaagacaa tctgcaacca cactgcttgc actcctgata ggctgtgcgg ttattcctgc
     3841 cacccatgcc tactctgtga ccgtagtggc cagccagcct gtcaccacac aaatcatgcc
     3901 ggagctggcc gccattcagg gcgtgatggc agagatggtc cgtatgcaga cagccaccgg
     3961 cacagccatc aaccagagtt cagagaagct ggcctctgtc atcgcacagg acgggcaggc
     4021 cacccgtcag cagatgatat tcagtaatga aacccgccgg cttgaggaag cccggaagag
     4081 cttcagcgtg ccggactcca tctgcagtga atccgcctcc ggagttgcag ctgaaagtcg
     4141 ccgtgcagca gcgtctgcgg ctgcacgtct gagtcaggga ggaggcgtca gcagtaaacc
     4201 catccgtgaa cgtctctccc gggcagcaga ctcaccaacc cgcgaggcat atgacagtgc
     4261 aggcattcat gccggttact gcacggaagc tgaatatgcc cgctttggcg gaaccgatgt
     4321 ctgccctgcc gtgggcgagc tgccgggcgg agacagccag gtgcgatcgc tctatcaggg
     4381 tgcaggcaca gcagataccc ctgccgccct cacctgggat cagaaacaga ttgatgctgc
     4441 cacggcttac atgaaaaaca ccgccagacc gtcagcgggt cgtgccccgg ggaaaggtga
     4501 agtgggaaca cagaccgggc gcacgtatgt gggcctgcag aatgaataca acggcatcat
     4561 cgatgccgcc tctcatccgc aactgtcact gatagctgac agtaccccta atgaggccac
     4621 ccgtggagca ctgactgaag ccctgcagtc accatccgct gcggcatatt ttgaccggac
     4681 cgcatcatca gaggcccgca cccgtggtca catgagccag cgggagtttg aggcctttga
     4741 agccggcaga cgctatgcaa acaccgactg gcagcaggat ctgcagggca tggaaggcga
     4801 taaccttctc cgtgaactgc tgcgcaccac cgccctgctg aactggcaga tgaacgacct
     4861 gaaagagcag atacgccagg gtaatgttat cgccgggcag cagctggcac tggcggcacg
     4921 acagtattac ggacagcgtc tcggtgaact gtcacaggcc atgagccagg ggagcgtaag
     4981 atgaaagacg acaacagcac cgacaaaaaa aaaccggccc cccgtctccg ccgcatcggt
     5041 gacatcggga taaacctgtt cgcaccactg gccgaaaccc ggtggatgct gcgcagctgc
     5101 cgccatatgc tgggccagca taaaacgcgt ctgaagcgcc tcatgcccga ccggcaaccg
     5161 gagattccgg tctgtatgac ctgggaagaa gccgtcaggg ccagcggcct tacggtcaac
     5221 gaactggatc ggcgtctgcg acgtcgcagg atgttctggc ggacaggatt ctggctgatg
     5281 atgataccgg tacttctgct gtgtggtatg gcccttgcgg ccacagcgct tccggccatc
     5341 accctgatac gtatactggc ctgcctgtta ctgatgagtg ccattgcagc actgtgtgcc
     5401 tcaaaaaccc tgattgtgtc ataccgcctc tggcaactga atgaacggcg ggtcagcgag
     5461 gacgaacagg gcaccttccc cgattacctg caggcccggc acggctggaa acaggccaca
     5521 tggctggcca ttacccaccg gcagtactga ccgtccggtc tgcaattcct tttcctcatt
     5581 ccggaggctg atgccctgca tcccttcggt tccggagtta ccgtgaaaat catacccaga
     5641 gcactgagtg cgggtctggc gctggctgcc gtacctgcac aggcattcgt cacctatcag
     5701 gacattgtga gcgccgccac caatccggat gacctgtcgc gccaggcact ggtcaccatt
     5761 tttggcgacg tggtcacaaa tcccctgaca gccagcagca acaccattat cggcaacctg
     5821 ttcatggtgt ttaacgcaat tatcgccatt atggggatag tctggttcct gtttattgga
     5881 atccgccatg ttgtccgcac cggccaccag ggcaatgtct ttaacaacgg acgggacgtc
     5941 gtcggaatgc tcagtattgt gagcggtttt atgatgattg tgcccaccgc ttccggctgg
     6001 agcctggcac aactggtcat gctctggggc gcatccatca tgggcatcgg ttccgccaat
     6061 atcatggttc agactgctgc agaaaacatc gctgaaggct attcaatgac cgtacagcca
     6121 gtacatgtct ccacccgcac ggcagcacgg ggaattctgg agatggcgct gtgcaaacac
     6181 gccatgaata cgggcctcga tgactttaac cgtaccgcac gttcatcaac gccgcccatg
     6241 agcgaatcct cacggacaga gaacggcagg tataccgtca ccgtttcaaa tggcagtgcc
     6301 acctgcggga gtgtgagtct caccgtgaaa gggaatggca gcaacaacca gtccgcgctg
     6361 gactattttt tcaatccctt cattgaaacg gagtatcagg gagtcctgac ggcacaacgc
     6421 tccgccatgg ataccatgct ccgtgacctg gacagcgcag cacaggaatt tgtgtcagcg
     6481 taccggaccc gacgcgacac gggtaacggc aacctgccgg atattgaaag ccgtattcag
     6541 aaggcagcca accggtatga agaaacggtg cagaaggcgt tgccggcaga taacgcagaa
     6601 actgagcgca gggagcagct gaaatcctac ctgaccacct acggctgggt tgccctgggt
     6661 gcctggtacc agacctttgc caccgccaac cagcgactgg aagacctggc caaccgcgct
     6721 cctactgtga gtggcgtctc gtcactgggc gaagccgggg atacggagtt cctgaaggaa
     6781 gtcagaggag cttaccaggc acagttgcag aactcagcgt atactcctgc actgggaacc
     6841 gccacctctg ccagtgaata ccacgcatct aaaactcaag accctcaatc agcaatctta
     6901 aaattgattg ggtttaacct aacaagaatg actaactgga cagccactta tctgagcggt
     6961 acaggtacca caactaatca actaaatccg ttgataaaaa tgaaaaacat tggcgattac
     7021 accatggtta caaccgaaac catctgggcg ggttatacca ccgtcagggt actttccacg
     7081 gccggcagca actccgtttt tggtcgactg gtcaatgccg tcagcggatt atctgacgcc
     7141 gcaaaggctt ttctggaagc agccgctcca ccgctctact tcctgctgtt tttgctgttc
     7201 tgtgccggct tttcgttatc catttacctg ccgtttattc cgtttatctt ctggatgacc
     7261 ggcattggta actggatagt tagcgtactg attggatgtg ctgctggccc cctctgggcg
     7321 gccacccatc tgggaacaag ccaggaccgg ggaagccggg ccgcttacgg ttacatctac
     7381 ctgatagaca gcatgatcag accgctgctg atggtattcg ggttcttttt tgcatcggtc
     7441 gccatcgtgg ccggcggcac aatcctgaat gcgctgtttg ccgccgcgct gatgaatgtc
     7501 cagataaatt ctgtgacggg cgtgttcagc atggcaggat ttttgatgat ttatgcccgc
     7561 gtctgtacca cgctggttgc cgccgttttt gcacttcagg cttatctgcc tgaccatgta
     7621 attaattttc tgggcggacg tgacggcgtt aatatgctgg ggaatatggt ttcttcagtc
     7681 aaagatatta ttgccggagg taataccaat atgcgcagaa cacctggcgt tcgtgaagaa
     7741 aaactgaaaa atattaatgg agatgataaa gacggaataa aacaataaat gaaaatatcg
     7801 aaaaaccaat aaaacactat tgccatattc acctgaagag attaaaatat attcaggtga
     7861 atatggaggt ttattatgaa cacaacaaaa aacagatatc cttcctggtg ggcatattat
     7921 taccttgttc gcaatgctta ttttatattt ggtgttcctt ttttattact atgttctcta
     7981 ggatttaccg gcatgatgct tacagagcca tatatagaaa cagatattca agcatatcta
     8041 tatacttatg gcagttggct cctattaata ctcccatcac tctggctata tatgaaaacc
     8101 agagcgaaaa aaaagaaagt ccagcaggtt gttcagcaga taaaagaaag cagtcccttt
     8161 gcgccgacca gcgattatga gcaactgtca ttctcgaaga gttgttattt cgggatcgac
     8221 attaaaaacg gcaccatgct gtatgtgcgt atttatccaa acaatgtgat ggatgtgatt
     8281 ggtctggata ttcacaactt tacccgcacg gttgctgaag acggcaagct ggaaatccac
     8341 accacctatg tgagtctgcc gatgatccca ctggaaatct ccggcatctc atcaaggacg
     8401 ctggccaaca ccatgcacac catggctgca cgtggatatg agtacaagga acgtttcccg
     8461 cagatgattc ggtatcgggt taaagagtgg gaaaaagttg ccggtgttcc tgttgcagaa
     8521 gtattttaag gtagcgacaa actgttagac gataatccgt cagccggttt tctctgaacc
     8581 ggctttttct tttcaggcag aatgcctgtc tgccgtccct gtatcagact gtaatttgca
     8641 caatacggca atttgccgta cagtgtattc aggaggtaca aatcatgcca tctgcaacac
     8701 aaactgcccg actggaagca agaatcagca cagaactgca tgctatgctg aaacgcgctg
     8761 cagaaatcca gggacgcact atgaccgatt ttatcatttc tgccgttcag gatgcggcac
     8821 aacgcgctat agaacaaacc gatatcatcc gactgtcccg gacagatcag gaaatttttg
     8881 ctcaggcatt actgtcacca acagaaccta atgatgcgct caaacgggcc tttaaacgcc
     8941 gtaataatat tctatacaca aaataaatat aatgtttcag ctgaaaccat ttaacagcac
     9001 atatgacaga acctcatttt tatgtggttc tgatgctctg gaccgttata taaaagagca
     9061 tgtcacacag gatattcgac gacgcatcgc ggcctgcttt gttgctatag ataacgatca
     9121 tcgtatcatc ggatactata cgttatcagc ggccagtata ttattaactg acttacctga
     9181 gcatttgaga aaaaagctac cacgatatcc taccatcccg gctattcgta tgggacgcct
     9241 ggcagtaagt ctgtcatcaa aaggaacagg acttggtggg acacttttag ctgatgcgct
     9301 atatcgtgca tacacatctg aaattgcagc atatgcgctg attgttgatg caaaagatga
     9361 cacggcagtc tcattttaca aacattatgg atttatgacg ctccctgagt ccgggcgaac
     9421 tttattcatg ccactggcat cattatctgc aatagaataa ccagaataaa tccgggtaaa
     9481 aaagggcgca ttgaggtcag ccttcgcaac aatccggcgg cagattccct gccgccgtgt
     9541 tgcccgttaa cgtttaactt cgtaggctaa cgttgccaca agcactgtgt ttccctgctc
     9601 gacacggaat ccgcaaagcg aatccctcac catccagaca aaacacagta tcgtcacaca
     9661 gacgacgatt aacattatta aaaacgttcg ttgtggcatc gcagcccctc cattgccttt
     9721 cggccgtcat cgggctacta taatgttgtt gaggcattac agtgggccct gggttgattg
     9781 ataaaaagat cgcctggggc tttctgctgc atgccgtcca caaaacgctt tcggcataca
     9841 gcctcaagcg cccgcagcta ttctaattgt tttcctgtcc gccacaatcc ttttcatgcc
     9901 gttatatttt ttgtatccgg aacaggaaaa tacccgggaa ttatctcatc cgaacgcctc
     9961 aggttttccg tctacgggct tcgtgttatt accccaaaat gcaaaatgcc tgaaataagg
    10021 agtagtcatg aaagacgaaa tacaaagtat gtgcctcacc ccggatatcg ttaaacagtt
    10081 caatactctt ttgaatacac actgccagtc ctcagaagag gcgctcggca tgctcagcgc
    10141 actgtgtttt gagctcatga tgaaatgtgg tgttcagtcc ataaacagcc tcatggtcaa
    10201 cggtatgggt atatccatgc aactccagga gattgatatt gccgaaaggc caggcaagaa
    10261 tattcactaa cctgaacgga gcctgaaaac gctgattttt caggccccag caggctaaaa
    10321 ctgatgatgc aatgacaaca aaatttctgc gtttgtaccc cacagatgaa aaagataaaa
    10381 actcacaccg gacttctcgc cagtaaagat aaaaaccggc gcgtcagcct ctacgaaacg
    10441 ccaactgcat ggtgtatcag ggggcaggag tgctacagca aaagcaccgg acgccgctgt
    10501 ggcagccacg acagtctgag ccgactgcgc ctggacagca ttaagcccgt tgaatgagtg
    10561 gtggcaccat gaaagataac aacaccaccg ccacccctga agcgtggaaa atgcgttttc
    10621 tggccaacgg gacatccggt acatggtttt acactgacga cattgctgtc actgacgcgt
    10681 ggcttcgcgc acatccggat gatgtggagg ttatccctct ctgccggata ccgcccgggc
    10741 agcattccca tgaacaacat attgaagaac tccgcgcggc cctggcccgg atgctccata
    10801 tgcatcatat gatgctggaa accacgcatt accgggaaaa catcaccgat gcccgttgta
    10861 tccgtgaaat gcatgaagcg ccattacagg cgctcaatat tctgtccaga tacccgcata
    10921 aaaaggacga ggtgtgacgc atgtggctac tgattttctg gctttcatcg ccctttaatg
    10981 tctcttccgg tgatgcccgt cagcccacac tggcggtgca cagccaggcc tttcataccg
    11041 aacgggcctg ccgcgatgca ttgcgcgcca ttaaaaagga gaatgttggc gaattttctc
    11101 tgcggggcgt ttgtgtttct gacggaaata cgaaggagga ggctgagagc tcatgacgga
    11161 tattactgac agacatacac ttcgccgtat gtcctggtcg gaactcatca cggcaggaca
    11221 ggaagcagaa tttcagcgtg attacgagcg taccagaata gtgtggtcat tcgccttaca
    11281 tgttgcaaca accacgatta acaaaaatct ggccatagct catattcgac gctgcgatac
    11341 tttgttgcat aaatcaaagt ctgtacccgg aaacaacccg tgtggttccg gtcagcgagt
    11401 ctgagacctc aacatcccgg gtgatgattg tcattccgga ctgacgcaac cgctgcacat
    11461 ctgcggcaat acgctgaata ccaagccaga acagaccatc cagcgcggtg acaattaacc
    11521 cgctttccag cgccagtttc agccgtgctg tcggggcttt aacctgcccg gctatatcag
    11581 tgaaacaacg cgtcgtcatg ccatagctgt ccacccgccg tattcgcgtc gcaagactgt
    11641 aactgaagct atccggcacg ccggataagt cattctgcag actgtataca catccccgct
    11701 gccgctcatt gaatacgact tttttctggc tgagctgcca ttcccgccgc acgctgtcaa
    11761 tcatctgcgg tgtccgggtt aacagcagac gaaacggcag ttcaaaactg gtcacataat
    11821 caacattaag taaggcaatc cgcagccgct ccctggctcc tgctccggta tcacggcatt
    11881 cacgcatgac atcttcccac tggcgcatca gtcgcggtga ctcattcctg gccctgaact
    11941 gatatttctc aatctgataa tcaatatgtt cctgcatacc gtcaccggat atctgttgcc
    12001 gtatcatcac ggatatcagc gtactggaat tcatgccctg cgtgaagtat cacactgcca
    12061 aaaaaaacac acctgaacgt tattatttcg caatattaat tgcacctgtt ttcataaata
    12121 aaaaggagac gatgaaatga taaaatatat cttttcactc accattattt tttccgcttc
    12181 tctctgtgcc gcacagattc aggggcacgt catccgcgtt cttgatggtg atacgctgga
    12241 aatattacag gacaaaaagc ctgtcagggt ccgtctggct aatattgatg caccggaaaa
    12301 gaaacagccc tttggacgct ggtcagccac tcagctgaag gacctggtgg ccggaaagcc
    12361 cgtaaccgtg caatacgcgc agacagacag gtatggccgt attctcggcc gggtctatac
    12421 cgctgacggc acagaagcaa atcgctggca ggtccgtaac ggcgctgcct gggtgtaccc
    12481 ggactacaat aatgactata ctctcccgcc gctccagcgt gatgcccgcg ccctgaagcg
    12541 gggattatgg gcagataaaa atccggtacc accatggacc tggagaaaac agaataagaa
    12601 ataattattt gctgatatga agaaaaaaac catttcaaag aaaataaatg gaaaaaatac
    12661 agtatgggaa aaaattgcca tttttaccgc cgcgttattt ttaatatttg ctgtctgctg
    12721 gtcgtaataa gcagaataac agaaaggtgt aaaaatgaac ctggttgatg catttgtcac
    12781 taaagtgata tccggaccgt atgaagaata cggaaaatgg tgggtggatg tagaatatat
    12841 ttcctgggga gttccgggta aaagccggct catgtttggc tcacgggaac aggcacagga
    12901 agtgcaggaa ggttttcgat ttttaacctg agcatatttt gttaaagggc agaaggaggt
    12961 gaccctctgc ccttaagtac gtttccggga ttatcagtca accagttccc ggagagctat
    13021 cactgcgtta gccacagcaa caaccgccct gagaacgatg attaacaggt taatcatccg
    13081 tttctccgtc aatggaggaa atcactgctg cttactctta cattgcctgt cacaacagct
    13141 ctctcattgt tagctgaaaa gagacttcct ccggtcagag ccaccaactc caccccggaa
    13201 tcgcaaaaaa accggcctca ccagtcggtt tttttgtacc tgtctcccac caccttctgc
    13261 cgttcgctct tctctgtaaa aatgtcctgt tgaattcatt cctgtccggt tattaccggc
    13321 gctgattcgc attcatgcca cccgacttaa tcccgaaatt tatccattca tctatggagg
    13381 aatccccgtg tcttaccaac agacgtctgc cgccgaagat ccaatggcta tatggtatat
    13441 cgtcggtgcc atatgcctgc tctttgcgat tatcatctgg cgctttttac ctgaaatcgt
    13501 cttcgcctcc tgcctgatcc tgcacacact gtggggcatg attgactggg ggccctttca
    13561 caactttgcc gccccccgtt acaacctgct ggccatcact gctaacaacg cggcaaccat
    13621 cactttcagc cagtggctgg atgttatgtc ccggacagtg gggatcctct ggctgattct
    13681 gcttcccatg acattcggct ttctgtggat gtggtttcac catccggccc agccccgttt
    13741 tacccgcagg ccactgaata tccacacact gccccatatt ttttcagctc tgtcaccggc
    13801 gatcgccccc gttctggctg acggagacaa caacagactc ttccacggac aaaaacgtcc
    13861 cgagcgccgt gtcgcactaa cccccgaagc ctttgtggag cagaacaacc ttatccgcaa
    13921 catgcagctc gatgttgcgt ccacacgcca gtgcttcatg gcgcaactgg ggcaaccact
    13981 gaccagctgg aaagacatgg ccccacatga aaaagcgctc tttgccatct ttggcctgca
    14041 gttcttcctc ggtgaccgga aagcagcggt agccctgatg aacaacctca atctctcctg
    14101 caggctcaaa agtaaacggg accaggggcg tttcagcacg ccggtgtatt cactggccag
    14161 aaacgccttt atccgggtca tcaaaacaga aggcgcacaa aagtggctca ggcagcaccg
    14221 ttacgttcgc agcggtctgg tctggcttta cgcacatgat ttgcgcctga cgcctccgaa
    14281 ctggctgtgg ctcaagggcg ttgaccggac gctcttctat gcccttcacc gggcaaacac
    14341 cacaaaaggc ttcatcgaag gggccggcgt ggtggccgtt gcccgggcag aaaatgaagc
    14401 gtgtcgtctc ggcctgccct gcccggaacc ctgtgtggaa gaggccattg aagggcttcg
    14461 acgggacatg ctggggctgg gcctgatatg ggacgaacct cagccagatc gcgatcgtaa
    14521 acgtcagatc aggacccgct ggtcactcac ggatgacgtg ataccccgcc gccatgataa
    14581 cgacgaagac actgacactg g
//
LOCUS       DBBGLQ010000110        14571 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.110, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000110 DBBGLQ010000000
VERSION     DBBGLQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.110"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(172..1365)
                     /locus_tag="ACIYSB_004314"
     CDS             complement(172..1365)
                     /locus_tag="ACIYSB_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5431232.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTKAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPALALVMLLRLPGTPPQPTPERKSVSLKRDF
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFATDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLAEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLACSHTLGYGLCALLVPLVSKTGVAIALIVMALFAAVLFSMVTVFIWR
                     CCKSK"
     gene            1501..3225
                     /gene="iucA"
                     /locus_tag="ACIYSB_004315"
     CDS             1501..3225
                     /gene="iucA"
                     /locus_tag="ACIYSB_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HGC5431233.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3226..4173
                     /gene="iucB"
                     /locus_tag="ACIYSB_004316"
     CDS             3226..4173
                     /gene="iucB"
                     /locus_tag="ACIYSB_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HGC5431234.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRDSGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4173..5915
                     /gene="iucC"
                     /locus_tag="ACIYSB_004317"
     CDS             4173..5915
                     /gene="iucC"
                     /locus_tag="ACIYSB_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HGC5431235.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYST
                     LLGDLQLLKARRGLSASDLINLSADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENDQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            5912..7245
                     /gene="iucD"
                     /locus_tag="ACIYSB_004318"
                     /pseudo
     CDS             5912..7245
                     /gene="iucD"
                     /locus_tag="ACIYSB_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            7271..9472
                     /gene="iutA"
                     /locus_tag="ACIYSB_004319"
     CDS             7271..9472
                     /gene="iutA"
                     /locus_tag="ACIYSB_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HGC5431236.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVSKKGQPETM
                     KEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAVFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTIVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYGYKGRGRTFGLSYSVLF"
     gene            complement(9537..10753)
                     /locus_tag="ACIYSB_004320"
                     /pseudo
     CDS             complement(9537..10753)
                     /locus_tag="ACIYSB_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11071..11695)
                     /locus_tag="ACIYSB_004321"
                     /pseudo
     CDS             complement(11071..11695)
                     /locus_tag="ACIYSB_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(12062..12940)
                     /gene="sitD"
                     /locus_tag="ACIYSB_004322"
     CDS             complement(12062..12940)
                     /gene="sitD"
                     /locus_tag="ACIYSB_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HGC5431237.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGFPLAGGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGVTIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLALGISVMVSMTGVWLSFFIDSAPAPTIVILFTVVFILTFTASSINAHAKENV
                     DPQDLPTSTTTREQHL"
     gene            complement(12937..13794)
                     /gene="sitC"
                     /locus_tag="ACIYSB_004323"
     CDS             complement(12937..13794)
                     /gene="sitC"
                     /locus_tag="ACIYSB_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HGC5431238.1"
                     /translation="MNVLLEPFSYEYMLNAMWVAAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIAPADILQLTIIGIVSVIVLFFKWKDLMVTFF
                     DENHARAIGLYPGRLKILFFTLLAVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLVIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRQR
                     AHKALEDRS"
     gene            complement(13791..>14571)
                     /gene="sitB"
                     /locus_tag="ACIYSB_004324"
     CDS             complement(13791..>14571)
                     /gene="sitB"
                     /locus_tag="ACIYSB_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HGC5431239.1"
                     /translation="RNGQKALRDASFTVPGGSIAALVGVNGSGKSTLFKAIMGFVRLT
                     TGKISVLGIPTRQALQKNLIAYVPQSEEVDWSFPVLVEDVVMMGRYGHMGMLRIANKR
                     DRQVVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQGDVILLDEPFTGVDVK
                     TEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGTVLASGPTDTTFTAENL
                     ELAFSGVLRHVTLKGSEESIITDDERPFVAYRPSAVQREER"
BASE COUNT         3569 a         3824 c         3722 g         3456 t
ORIGIN      
        1 actggtaagc ctcatacatt ttctttcact caagttgtgg aagccctctt tcgtcgaacc
       61 gttcgttata aatgcaaaag atgtatgctg accctctcaa agccgaaatc atacagagtt
      121 tatggaaaag tggtgaacca gtcgcgtcga aagtagattg agctcatatc attacttgct
      181 tttgcagcag cgccagataa atacagtcac catgctaaat aatacagcgg caaataatgc
      241 catcacaatc agtgctattg cgacacctgt tttcgacacc aatggtacca acaacgcgca
      301 aagcccatag ccaagtgtgt gactgcaagc gagccagccc gcgcctgcgc cgtcggccag
      361 cttatcattc agtaaaagtt gataagcggg ggttgccaga gcagctccaa atgacagcac
      421 tgcacagcct aggtaaaaca accatagctg ttcagcgagc atgatagcca gaccactact
      481 catcaacacc ccggcactca ggagcagagc cactggagtc aggcgctgtg gacggagtac
      541 cacgaactgc gcgataagcg cagctattgc ggacagcccc aacaaccacg ctacctgttg
      601 gctaatagtg gtggtatcag tggcgaactg gcgagtaagg gctggcgaaa gtccaagctg
      661 catcatgctc attgccgcag ccagtaacat tgcgcaaagc agataaggca ggaaatcccg
      721 cttcaggctg acgctcttgc gctccggtgt gggctgtggt ggtgtgccgg gcaaccgcag
      781 aagcatcacc agtgccagcg ctggcgctgc catcagcatc cacactggcg ccagagggtg
      841 aatgaccaac attgccgccg cgcacagcgg cccgaatagc ctgccgcagc tcaggccgga
      901 gctgatggtt gccagagcgg ccatgcgatt cccttctccc gctctctgca atgcccagac
      961 ctgacaggct ggcaccattg ctgacacggt cagaccgtag acgatccggg cgatgattaa
     1021 tattcccaat cccaccgctt ttgttacggc accggtagcc atcagcacgc ttcccagccc
     1081 aagcagggta aagcttacgc catatcctcc cagagcccac agcaccacag tcttactgcc
     1141 agtaagggaa atttgctttc cccaccatgg ggaagatggc agaaacagca ttgagcccag
     1201 catcagcagg gcagcccata cagacagact cagatttgtc tgtatgacca gaacagggag
     1261 tacaaccagc agaccgttct gtccgatacc gagaagaccg gcactgaacg ccagtggcca
     1321 gcaggacagg gttttttggg gcgtatcttc gatcttagat gacattgtaa tgtttcaaca
     1381 ccatgtatta attgtgttca tttgtaaaat taatttttct gacaataaca tttctcattg
     1441 ataatgataa tcattattga cataattgtt attattttac tgtgtaggag ctgtttgatt
     1501 atgatcctgc cctctgaaaa atccgccaca gatgtggctg cgcagtgctt cctgaatgcc
     1561 ttgattcgtg aaaccaaaga ctggcaactg gcggaatacc cgccagacga attgattatc
     1621 ccgctggacg aacagaagtc gctccatttc agagtggctt atttctcccc aacccagcat
     1681 caccgctttg catttcctgc tcatctggtc acggcatcag gcagttatcc tgtcgatttt
     1741 accactctct cccggcttat tattgacaag ctccggcatc aactttttct gccagttccc
     1801 ctctgcgaaa ctttccacca acgcgtgctg gaaagctacg cccatacgca acagacaatt
     1861 gatgcccgcc atgactgggc catcctgcgt gagaaagcgt tgaattttgg cgaggctgag
     1921 caggcactgc tgacaggaca cgctttccac cctgcaccta agtctcatga accgtttaac
     1981 cgccaggaag ctgaacgata cctgcctgac atggcacctc acttcccgct gcggtggttt
     2041 tcggtggata aaacgcaaat cgctggtgaa agtctgcatc ttaaccttca acagcggttg
     2101 acgcgatttg ccgcagaaaa tgcgccccag ttactcaacg aattaagtga caatcaatgg
     2161 ctgttcccgc tgcacccgtg gcagggagaa tatcttttgc agcaggtgtg gtgccaggca
     2221 ctttttgcta aaggacttat cagagactta ggcgaggccg gcacgtcgtg gctgccgacc
     2281 acctcttccc gctctctcta ctgtgctacc agccgcgata tgatcaagtt ctccctgagc
     2341 gttcggctga ccaactccgt ccgtactctg tctgtgaaag aggtggagcg aggaatgcgc
     2401 ctggcacgtc tggcgcaaac cgacggctgg cagatgctac aggcccgctt ccctactttc
     2461 cgggtaatgc aggaggacgg ctgggccggg ctgcgcgatc ttaacggcaa catcatgcag
     2521 gaaagtttgt tttccctgcg tgaaaacctg ctgctggagc agccgcaaag ccagaccaac
     2581 gttctggttt ccctgactca ggccgcaccg gatggcggtg attcgctgct ggtttcggcg
     2641 gtaaaacgcc tgagcgatcg cctcggtatc actgtgcaac aggccgccca cgcatgggtc
     2701 gatgcttact gtcagcaggt gctgaagcct ctgtttacgg ctgaagcgga ttacggcctg
     2761 gtgctgctgg cgcatcagca aaatattctt gtgcagatgc ttggggatct gccggtcgga
     2821 tttatttacc gtgactgcca gggcagcgcg tttatgcctc acgcgacaga gtggctcgat
     2881 accattgacg aggcgcaggc ggaaaatatt ttcacccgtg agcagttgct gcgctatttc
     2941 ccttattacc tgctggttaa ctccactttt gccgtaactg ctgcgctggg tgctgccggg
     3001 ctggacagcg aagcgaatct gatggctcgt gtacgaacat tgctggctga agtgcgtgac
     3061 caggtgactc ataaaacctg tctcaactat gttctggaaa gtccgtactg gaacgtaaaa
     3121 ggtaactttt tctgttatct gaacgatcat aacgagaaca ccatcgttga cccttcggtg
     3181 atctattttg atttcgctaa cccgctgcag gctcaggagg tctgaatgtc tggggcaaac
     3241 attgttcaca gcggatatgg actgcgctgt gaaaaactcg acaagcctct gaatcttggc
     3301 tgggggctgg acaatagcgc ggtgttgcac tggcccgggg agctgccaac aggatggctg
     3361 tgcgacgcgc tggatcagat atttatcgcc gcaccacaac tttcagcagt ggttcttccc
     3421 tggtccgaat ggtgtgagga gccacaggcg ctgacgcttt tcggacaggt acaaagcgac
     3481 attatccatc gttccgcttt ctggcagtta ccgttatggc tgagttctcc ggcaaaccgg
     3541 gactccggtg aaatggtttt tgatgcagag cgtgagattt atttcccgca gcgccccccc
     3601 cgtccgcagg gtgaagttta tcgtcgttac gatccacgca ttcgcaggat gcttagtttc
     3661 cgcattgccg atcccgtttc tgatgcagaa cgtttcactc gctggatgaa cgatccgcgc
     3721 gttgagtatt tctgggagca aagtggctca ctggaggtac agatcgccta tctggaacgc
     3781 cagctcacca gtaaacatgc attcccgctg attggttgtt tcgacgatcg gccgttcagt
     3841 tatttcgaaa tctactgggc ggcggaagac cgcattggtc gccactattc gtggcagccc
     3901 tttgaccgtg gcctgcatct gctggttggt gaacagcaat ggcgcggagc ccactatgtg
     3961 caaagctggc tgcgcggggt gacacattac ctgctgctga atgagccacg tacgcagcgc
     4021 acagtactgg agccacgcac cgataaccag cgcctgttcc gccatcttga gcctgcggga
     4081 taccggacaa ttaaagagtt cgacttccca cataaacgct cgcgcatggt gatggcggat
     4141 cgtcatcact tcttcacgga ggtcggtctg taatgaatca caaagactgg gatttggtca
     4201 accgccgctt ggtggcaaaa atgttgtctg agctggagta tgagcaggtt ttccacgcgg
     4261 aatctcaggg cgatgaccgc tactgcatta acctgccggg agcacaatgg cgcttcatcg
     4321 ctgaacgtgg tatctggggc tggctctgga ttgatgctca aactctgcgc tgcgcggacg
     4381 agccagtact ggctcagacg ctgctgatgc agctaaagca ggtactgtca atgagcgatg
     4441 caaccgttgc tgagcatatg caggatttgt attccacgct gctgggcgac ctgcaactgc
     4501 tgaaagcccg tcgcgggctg agcgccagtg acctgattaa tcttagtgcc gaccgcctgc
     4561 aatgcctgct gagcggtcat cctaaattcg tttttaataa aggtcgccgt ggctggggta
     4621 aagaggcgct ggaacgatat gcgccagagt atgccaacac ctttagactg cactggctgg
     4681 cggtaaaacg tgaacatatg atctggcgct gtgataacga gatggatatt catcagttgt
     4741 tgacggccgc aatggatccg caggagtttg cccgcttcag tcaggtctgg caggaaaacg
     4801 gactggatca taactggctg ccgctgcctg tacatccgtg gcagtggcag caaaaaatcg
     4861 ccaccgactt catcgctgat tttgccgaag gcaggatggt gtctctcggc gagtttggcg
     4921 accagtggct cgcccagcag tcgctgcgta ccctgaccaa cgccagtcgc cggggtgggc
     4981 tggatatcaa gctgcctctg accatctaca acacctcatg ctaccggggg attcctggca
     5041 gatacatcgc tgccggacca ctggcttcac gctggctaca acaggttttt gcgaccgacg
     5101 ctaccctggt gcaaagcggt gcagtgatcc ttggtgaacc ggctgcaggc tatgtctccc
     5161 atgaaggcta tgccgcgctt gcccgggctc cctatcgcta ccaggaaatg cttggtgtta
     5221 tctggcggga gaatccgtgc cgctggctga aaccggatga aagtccggtt ctgatggcaa
     5281 cactgatgga gtgcgacgaa aacgatcagc cgctggcagg cgcatatata gaccgctccg
     5341 ggctggacgc tgaaacctgg cttacgcaac tgttccgggt ggtcgtggtt cctctgtatc
     5401 acctgctttg ccgctacggt gtcgcgctta ttgcacatgg acaaaatata acgctcgcca
     5461 tgaaagaggg ggttccacag cgtgttctgc tgaaagactt ccagggcgat atgcggctgg
     5521 tgaaagaaga gttccccgaa atggactctt tgcctcagga ggttcgtgat gttacatccc
     5581 gcctgagtgc ggactactta atccatgatt tgcagacggg tcacttcgtg acagtactgc
     5641 gttttatttc gccactgatg gttcgtcttg gcgtacctga aaggcgattt tatcaactgc
     5701 tggcagcagt gttgagtgat tacatgaaaa aacatccaca aatgtcagag cgttttgcgc
     5761 ttttctcact tttcaggcca caaatcattc gcgtggtgct gaacccggta aaactgacct
     5821 ggcctgatct ggatggcggc agccgcatgc tgccgaatta ccttgaggat ctgcaaaatc
     5881 cgctgtggct ggtaactcag gaatatgaat catgaaaaaa agtgtcgatt ttattggtgt
     5941 agggacaggg ccatttaatc tcagcattgc tgcgttgtca catcagatcg aagaactgga
     6001 ctgtctcttc tttgatgaac atcctcattt ttcctggcat ccgggtatgc tggtaccgga
     6061 ttgtcatatg cagaccgtct ttctgaaaga tctggtcagt gctgttgcac ctacaaatcc
     6121 ctacagcttt gttaactatc tggtgaagca caaaaagttc tatcgcttcc ttacaagcag
     6181 actacgtaca gtatcccgtg aagagttttc tgactacctc cgctgggctg ctgaagatat
     6241 gaataacctg tatttcagtc ataccgttga aaacattgat ttcgataaaa aacgtcgatt
     6301 gtttctggtg caaaccagcc ggggagaata ttttgcccac aatatctgcc ttggtacagg
     6361 aaaacaacct tatttaccac cctgtgtgaa gcatatgaca caatcctgtt tccatgccag
     6421 tgaaatgaat cttcgtcggc cggaccttag tgaaaaacgg gtaaccgtgg ttggtggagg
     6481 acagagtggt gcagacctgt tccttaatgc attacgcggg gaatggggag aagcggcgga
     6541 aataaactgg atctcccggc ggaataattt taacgcactg gatgaggctg cttttgctga
     6601 tgagtatttt acacctgaat atatttcagg cttctccgga ctggaggaag atattcgcca
     6661 tcagttactg gatgagcaga aaatgacatc ggatggcatc actgccgatt ctttactgac
     6721 catttatcgt gagttgtacc accgttttga agttctgaga aaaccaagaa atatccgtct
     6781 gctacccagc cgctcggtaa caactctgga aagtagtggt cctggctgga agttgctgat
     6841 ggagcatcat ctggatcagg gcagggagag cctggaaagt gatgtggtga ttttcgccac
     6901 aggttaccgt tctgcgttgc cacaaatact tccctcactg atgccgctga tcaccatgca
     6961 cgataagaac acctttaaag tgcgtgatga cttcactctg gaatggagtg gcccgaaaga
     7021 gaacaacatc ttcgtggtca acgccagtat gcaaacccat ggcatcgccg aacctcagct
     7081 cagcctgatg gcatggagat ctgcacgtat tcttaatcgt gtaatgggac gtgatttatt
     7141 cgatctcagt atgccgcccg ccctgattca gtggcgcagc ggcacctagg gaaaacgcag
     7201 ccggaggctg cttctttaac tcgctacaca gcatctttgg gctgattttt tccgcccgtg
     7261 tggaggaata atgatgataa gcaaaaagta tacgctttgg gctctcaacc cactgcttct
     7321 taccatgatg gcgccagcag tcgctcaaca aaccgatgat gaaacgttcg tggtgtctgc
     7381 caaccgcagc aatcgcaccg tagcggagat ggcacaaacc acctgggtta tcgaaaacgc
     7441 cgaactggaa cagcagattc agggcggcaa agagcttaag gacgcactgg ctcagctgat
     7501 ccctggcctt gacgtcagca gccggagccg caccaactac ggtatgaatg tgcgtggccg
     7561 cccgctggtc gtgctggttg acggcgtgcg tctcaactct tcacgtaccg acagccgaca
     7621 actggactct atagatcctt ttaatatcga ccatattgaa gtgatctccg gtgcgacgtc
     7681 cctgtatggc ggcggcagta ccggtggcct gatcaacatc gtgagcaaaa aaggccagcc
     7741 ggaaaccatg aaggagtttg aggctggcac caaaagtggc tttagcagca gtaaagatca
     7801 cgatgaacgc attgccggag ctgtctccgg cggaaatgag catatctccg gacgtctttc
     7861 cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca ccctgcttga
     7921 taacacccag accggcctgc agtactccga tcggctggac atcatgggaa ctggaacgtt
     7981 gaacatcgat gaatcccggc agcttcagtt gatcacacag tactataaaa gccagggcga
     8041 cgacgattac gggcttaatc tcgggaaagg cttctctgcc atcagaggga ccagcacgcc
     8101 cttcgtcagt aacgggctga attccgaccg tattcccggc actgagcggc atttgatcag
     8161 cctgcagtac tctgacagcg ctttcctggg acaggagctg gtcggtcagg tttactaccg
     8221 cgatgagtcg ttgcgattct acccgttccc gacggtaaat gcgaacaaac aggtgacggc
     8281 tttctcttcg tcacagcagg acaccgacca gtacggcatg aaactgactc tgaacagcaa
     8341 accgatggac ggctggcaaa tcacctgggg gctggatgct gatcatgagc gctttacctc
     8401 caaccagatg ttcttcgacc tggctcaagc aagcgcttcc ggagggctga acaacaagaa
     8461 gatttacacc accgggcgct atccgtcgta tgacatcacc aacctggcgg tcttcctgca
     8521 atcaggctat gacatcaata atctctttac cctcaacggt ggcgtacgct atcagtacac
     8581 tgaaaacaag attgatgatt tcatcggcta cgcgcagcaa cggcagattg ccgccgggaa
     8641 ggctacatcc gccgacgcca ttcctggcgg ctcagtcgat tacgacaact tcctgttcaa
     8701 cgccggtctg ctgatgcata tcaccgaacg ccagcaggca tggctcaact tctcccaggg
     8761 cgtggagctg ccggacccgg gtaaatacta tggtcgcggc atctatggtg ctgcagtgaa
     8821 cggccatctt cctctaacaa agagtgtgaa cgtcagcgac agcaagctgg aaggcgtgaa
     8881 agtcgattct tatgagctgg gctggcgctt tactggcaat aatctgcgca cccaaatcgc
     8941 ggcctactat tcgatttctg ataagagcgt ggtggcgaat aaagatctga ccatcagtgt
     9001 ggtggacgac aaacgccgta tttacggcgt ggaaggtgcg gtggactacc tgattcctga
     9061 tactgactgg agtaccggag tgaacttcaa cgtgctgaaa actgagtcga aagtgaacgg
     9121 tacctggcag aaatacgatg tgaagacagc aagcccatca aaagcgacag cctacattgg
     9181 ctgggcaccg gacccgtgga gtctgcgagt gcagagcacc acctcctttg acgtgagcga
     9241 cgcgcagggc tacaaggtcg atggctatac catcgtcgat ttgctcggca gttatcagct
     9301 tccggtgggt acactcagct tcagcattga aaacctcttc gaccgtgact acaccactgt
     9361 ctgggggcag cgtgcgccac tgtactacag cccgggttac ggccctgctt cactgtacgg
     9421 ctacaaaggc aggggccgca cctttggtct gagttactca gtattattct gacaggaatt
     9481 ctttggaaac aaaggtatgt tgatataagg gccttccctg atcatggtgg gaaggctcaa
     9541 aacgccatat tcagtttccc gtagtggaac atcacgccca gcttaaagcg ttcccgattg
     9601 cggtatcccc tggcttttat cctcagcagc ctgagcttac tgttaagcgc ctccgcgttt
     9661 ccgtttgaga cacggtgtcg catggcattc aggatcccat acagcctttt tcctatcgtt
     9721 ttcgtggcgt ttttcatcat agtaatgtca ctgttggccg ccagagccag caatctctcc
     9781 cactcattac gtctttcctc acttcatggc ctgttccaga tattctttgc cagctctttc
     9841 agcgcccagc acaggcttgt cagcttcatc tgtgcacgca gccatatcag cttttcctgc
     9901 ctggattcgg tcatccattt atcgttgtac tgccacagga agcgggtccc ctttgcctga
     9961 cgccgacttt caacagggag gtgtggatgt tcatcctgac ggattttatc aaccacttca
    10021 ccaagttgct ttgctacatg gaagcggtcg aaggcgattt tctcaaccgc acagggtaag
    10081 tggatacgcg ctgctcttat acagcctgca ttcatgtcca ttgagagcgt tttgatagcc
    10141 agcaactgcc cgtcagtaag cgtgcgaaga tagccggcga ggctctctgt gccacgatcg
    10201 tccgttaagg ccagtgcacg accatcgcga tcggagatca cagttatata acaatgtcct
    10261 tttttaaagg cgacctcatc cacattcata tgacgggcgg ataatggctt ttttatccgg
    10321 gcaagacctc gcttaactgc ccgggtcata atgccgtcaa ccgcattcca gctgagctta
    10381 agttgtttcc ttacagcatc aacagtgctg attttcagcc atgagagaac gagtcgaaca
    10441 gcagcgtata ccgccttccg gggccagccc agggaactag caacgtctgg cagccatgct
    10501 ccgggcacat aatgcggggc acatcggctt cgacaatagt ggtgaactgg cagatatcaa
    10561 gatgacgcca tttacgatga cggtgatcgt gaacagaaca ggatttaccg caggtcggac
    10621 aggctaaccg ggcattttta gcgattccaa ctgtgacggt aacagaaccg gcgttttcat
    10681 cgagagaaag atatttaacc tgccatggag cggtcaggtt gagaatatga gcgtagaggg
    10741 acctttcgtc catggcggtg acctttggca attaaaacac catcatcatg ccttcagcca
    10801 ccataacaag ggaaaaccct gatatacggc cttaaaaaga tattccgtct ccggaacgga
    10861 ctgctcacca ctgaataaca ttatgcctgt tttatgtatc tttgactgaa atttatcgca
    10921 tatcctcatt tcattgcccg tatgaaaagg taagcgaaaa cctactgccg tagtagccat
    10981 tgagtagtat tggtaacata ggtgccaaca tttcaactga tctgcgctaa gatcttgggt
    11041 attctatgaa aaatgtggta gtgcttccaa tcagtagaac atgttctttt caatgacgga
    11101 accgatgact ttttcgttga ttttcacgga acgcgagaag cagattgttt tacgtgccag
    11161 ccgcttgatg cgggtgcgga ggggcaggtt gttaagttca ataggttgcg tgaatatttt
    11221 accggtcagg tatttctcct tcgtcacttc tctgacgttg cagcccctgt cattgctggt
    11281 cactatgccg atatagaacg aagtcaacag ggctaacagt tcccgacagg tctcatcatt
    11341 ccgtggcccg aaagaataag ccaacacacc gcagttttag tgttgtaagc agaccagtgt
    11401 tgttgcgcct tgctgccaac gaaatttaac tgctcatcaa gtttcaaaga taagggcaac
    11461 atcagcgtga acgactggcg tagatgttat ccgccggggc gaaaacattt ttaatgtccg
    11521 tatgacggta ttgatgccga ttttcagtgt tcatgggtat cacggacacc tgcgccgtta
    11581 tgtgccattt caacgatctg ctccttaacg ccgggtttcc tgacttcacc aagagtaagt
    11641 gagctggaaa acatgtttta catgagcgac aacggaaacg ctcatctcgg gaacgtgttc
    11701 ttaacggtta gactggcccc cctgaatcac ctgcagttga aggacagtaa caggttaagt
    11761 gataacagat gtctggaaat atagaggtaa atccaaggaa gtgccacttt tcatccgttg
    11821 ttgacaatac agcaaaactt gatgctattc gtacgcccac aatggaatgt ggattcaaac
    11881 tgaatattcc gtttgttgat atgagtgatt tgggcaacgg tggcaacaaa taactttata
    11941 caggagctga cggtgttttc ctgatcatgc cgcccatatt ttcttcggag gtcggggact
    12001 ttcaatacct tcccccgaca ctctatgact tcacaagatt gtctccatga ctgctatgtg
    12061 cctacagatg ttgctctctg gttgttgtac tagtcgggag atcctgtgga tcgacatttt
    12121 ccttcgcgtg agcattgatg ctggaagcgg taaacgtcag gatgaataca accgtgaata
    12181 agataacaat cgttggtgct ggtgcgctgt ctataaaaaa ggagagccat acaccagtca
    12241 ttgagaccat taccgatata ccgagcgcca gcaggagcgc aatatgaaag cgttgcgtca
    12301 ggagtaccgc aatggcgcca ggcgcaatga gcagagaaat ggagagaatg atacctaccg
    12361 ctttcagcgt cgccacaatg gtcagagaga ccatgcacag cagcccataa tgcagccatc
    12421 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
    12481 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatat
    12541 cgccgatggt taccccgagc atgtcgccga aaagaatatg gtcaagatgt acgtcgggtt
    12601 tgactgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccatca
    12661 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
    12721 ctccggcgac aaatgctcct cccgccagag gaaaacccag tatccacgca agcacgatac
    12781 caggaaaaac cgcatgactc atcgcgtctc ccatcagtgc ccaacctttc aggaccagaa
    12841 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
    12901 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcctt
    12961 atgcgcacgc tgacgatttg ccaaaagccc atgggtgggt gcaaagacaa atgctaaaag
    13021 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gctccgtcca gaaagtaact
    13081 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatca cgagcaggcg
    13141 gggaaaacga tcggttaaca accatgctgt tgcgccaggg gtcactacca gacagatcac
    13201 cagaaaagcc ccaaccgttt gcaatgctgc aacggtcgat accgccagca gtgtgaagaa
    13261 gaggattttt aatcttcctg gatataatcc aatagcacgg gcgtgattct cgtcgaaaaa
    13321 ggtcaccata agatctttcc atttgaaaaa cagcacgatg actgagacaa tgccaatgat
    13381 agtgagttgc aggatatccg ccggagcgat tgccagaata ttgcccagga cgatggtctg
    13441 aatatttacg gatgtcgggt ttaatgacac cataaacagc cccaggccaa aaaaggacga
    13501 gaagatcagg ccgatgatgg catcttcttt taagcgagtg cgttggttaa gaaacagcat
    13561 gctgcccgca gccagtccac cggaaaagaa ggcccccaga gaaaacggaa gccccagcat
    13621 atatgccccc gcaacacccg ggacgataga gtgcgacagc gcatcgccaa tcagcgacca
    13681 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca tcgccgcgac
    13741 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatctttct
    13801 tccctctgca ccgctgatgg tcgataagcc acaaaggggc gttcatcgtc agtaataatg
    13861 ctttcttccg agcctttaag tgtgacatgg cgtagtacgc cgctaaaagc cagctccagg
    13921 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
    13981 accgtataat cgcaaaatgt cgtgacggat ccgagattgt gcgttgagac aagcatcgtt
    14041 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tggcttcggt tttcacatca
    14101 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat cgctctcgcc
    14161 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgtcgatg gcgaaaatct
    14221 accatatcga cacgttccag cgcatcggtg acaacctgac gatccctctt attggctata
    14281 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
    14341 aaggaccagt ccacttcttc tgactgcggg acgtaggcaa tcaggttttt ctgcagagcc
    14401 tgccgcgtgg gaatacccag aacagatatc tttccggtag tcagacgcac gaatcccata
    14461 atcgctttaa ataacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
    14521 ccgcctggta ctgtgaagga tgcatcgcgc agtgctttct gcccgtttcg c
//
LOCUS       DBBGLQ010000111        14565 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.111, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000111 DBBGLQ010000000
VERSION     DBBGLQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.111"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..708)
                     /locus_tag="ACIYSB_004325"
     CDS             complement(<1..708)
                     /locus_tag="ACIYSB_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGC5431240.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGSLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHY"
     gene            complement(1265..2587)
                     /locus_tag="ACIYSB_004326"
     CDS             complement(1265..2587)
                     /locus_tag="ACIYSB_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGC5431241.1"
                     /translation="MKKFSVVIAGGGSTFTPGIVLMLLANQDRFPLRSLKFYDNDPAR
                     QEIIAEACKVLLKEQAPDIQFCYTTDPQVAFTDVDFVMAHIRVGKYPMREQDEKIPLR
                     HGVLGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNARILNICDMPIGIEGRMAQIVGLKDRKQMRVRYYGLNHFGWWTSIEDLDGNDLMPK
                     LREYVAKSGYVPPSNDPHTEASWNDTFAKAKDVQALDPQTMPNTYLKYYLFPDYVVAH
                     SNPERTRANEVMDHREKNVFSACRAIIAAGKSTAGDLEIDEHASYIVDLATAIAFNTQ
                     ERMLLIVPNNGAIHNFDADAMVEIPCLVGHNGPEPLTVGNIPLFQKGLMSQQVAVEKL
                     VVDAWEQRSYHKLWQAITLSKTVPSASVAKAILDDLIAANRDYWPELH"
     gene            complement(2584..4203)
                     /locus_tag="ACIYSB_004327"
     CDS             complement(2584..4203)
                     /locus_tag="ACIYSB_004327"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HGC5431242.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGISIMLRNPMFVGEALTA
                     PDSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVLVSFLTWNYFI
                     NAMGTTWGHFFGVDFSIEPTAGSGLTMIAGIKTLDTSIIGAIVISGLVTALHNRYFDK
                     PLPVFLGIFQGSSFVVIVAFLAMLPCAWLTLLGWPKVQLYIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFVYGPFIFGPAVVEGGLQVYWAEHLQAFSQSTEPLKTLFPEGG
                     FALHGNSKVFGSAGIALALYFTATPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAATMATVMYICGVVGNFGGGLLDQFLPQNWIPMFHHHSSMMFIQTGI
                     GLCFTVLYFVVFRTLILRLNLKTPGRESSEIKLYSKADYQAARGKTTAIEPDPCLRQA
                     AGFLQALGGADNIESINNCATRLRIALIDMAKTQSDDVFKALGAHGVVRRGNGIQVIV
                     GLHVPQVRDQVENLMKDSLSTKHTTMTEAVS"
     gene            4502..5236
                     /locus_tag="ACIYSB_004328"
     CDS             4502..5236
                     /locus_tag="ACIYSB_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGC5431243.1"
                     /translation="MIYKSIADRLRLRLNSIDFAIGSPLPGEKKLASEFGVARMTIRK
                     AVDLLVDDGLVVRRHGSGTYVARKDIHHETSSLTGLAEVLRKQGKEVVSRVIAFELMP
                     APPAIASILRIKIDERIFFSQRIRYVDDKPLMLEDSYMPLKLFRNLSLSHLEGSKFDY
                     IEKECGIIISGNYETLTPVLADTQLAGLMNVPEETPLLRITSLSYSDNGEFLNYSVMF
                     RNASEYQVDYHLRRVQARSSSDQEPE"
     gene            complement(5302..6072)
                     /locus_tag="ACIYSB_004329"
     CDS             complement(5302..6072)
                     /locus_tag="ACIYSB_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HGC5431244.1"
                     /translation="MNLQGKTALVTGSTSGIGLGIAQTLAKAGAQVILNGFGDVNEAL
                     NVVSRVGKTPGYHGADLSDESQIVSMMAYAWQEFGGVDILVNNAGIQYVSPVESFPVE
                     KWNAIIAINLSSVFHTTRLALPDMRARNWGRIINIASVHGLVASKDKSAYVAAKHGVV
                     GLTKTIALETAQTGITCNALCPGWVLTPLVQQQIDKRIEQGEDEESAHNALLAEKQPS
                     REFVTPEQLGELSLFLCSDAAAQVRGAAWNMDGGWISQ"
     gene            complement(6083..7474)
                     /locus_tag="ACIYSB_004330"
     CDS             complement(6083..7474)
                     /locus_tag="ACIYSB_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HGC5431245.1"
                     /translation="MSVLITLAALGLLMLAAYRGYSVILFAPIAALGAVLITDPEAVG
                     PAFTGLFMEKMVGFVKLYFPVFLLGAVFGKLIELSGFSRSIVAAAINILGRRHAIPVI
                     VLVCALLTYGGVSLFVVAFAVYPFAAELFRQSNIPKRLIPATIALGAFSFTMDALPGT
                     PQIQNIIPTSFFGTNAWAAPWMGLIGSLFIIVVGLIYLERQRHKAQVKNEGYGTELLN
                     EPETPDNINLPNPLLAISPLILVGLLNLLFTRWIPTWYGTSHQLVLPGLTAPITTDVA
                     KITAIWSVEAALIAGIVIVVSFGYRNIHGKLAEGSRTAVGGAILAAMNTASEYGFGAV
                     IAALPGFLVLQEALAAIPDPLLNEAISITTLAGITGSASGGMSIALAAMSDTFIAAAH
                     AAHIPLEVFHRVASMASGGMDTLPHNGAVITLLAITGLTHRQAYGGIFAITIIKSLAV
                     LFIIGAYYTTGIV"
     gene            complement(7824..9029)
                     /locus_tag="ACIYSB_004331"
     CDS             complement(7824..9029)
                     /locus_tag="ACIYSB_004331"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGC5431246.1"
                     /translation="MQDVVIVAATRTPIGSFLCSLAPLSAVDLGATAIRALLEKTAIA
                     PELIDEVILGQVLTAGCGQNPARQTALLAGLSNHTPAVTINQVCGSGLKAVIQAVQAI
                     RCDDAQIIIAGGQESMSNAPYFINGARAGIRLGHTSMKDSLVHDGLWDAFNDYHMGIT
                     AENLAQKYAISRERQDTFALHSQQKAASAIETGRFADEIAPVNVPQGKNPPRQVNTDE
                     QPRSGTQLEKLAQLKPAFLPGAGSVTAGNSSSINDGAAAVMLMRASKAAELGLPALAR
                     VAGYAVSGVDPALMGIGPVSATRECLKRCGWTLDDIDLIEANEAFAAQALAVGDELNW
                     DSDKVNVNGGAIALGHPIGASGCRILVTLIYEMARRNAQKGLATLCVGGGQGVALALE
                     RTTGECNVY"
     gene            complement(9039..9695)
                     /locus_tag="ACIYSB_004332"
     CDS             complement(9039..9695)
                     /locus_tag="ACIYSB_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HGC5431247.1"
                     /translation="MLTREQMAMRVARELRDGYYVNLGIGIPTLVANYIPDGMDVMLH
                     SENGLLGMGAFPMEEELDADMINAGKQTVTARKGAAIFDSAQSFAMIRGGHVDLTVLG
                     AFEVDVNGNIASWMIPGKMVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPNCTLP
                     LTGVGCIKRVLTDLALLDIADGTFILRERAPGVSIEEIVTRTAGKLFVPEHVPEMRFN
                     "
     gene            complement(9698..10405)
                     /locus_tag="ACIYSB_004333"
     CDS             complement(9698..10405)
                     /locus_tag="ACIYSB_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HGC5431248.1"
                     /translation="MAGLDKRVATYHEALEGLTSEMTILAGGFGLCGIPENLIAEVSH
                     RGVTGLTVVSNNCGVDGFGLGVLLETRQIRKVVASYVGENALFEKQVLNGELEVILTP
                     QGTLAEQLRAGGAGIPAFFTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGW
                     KADWYGNVIYRHTAQNFNPLMAAAGKITVVEVEEIVPPGELEPATIHTPGIFVNRVIQ
                     GKFEKRIEQRTLSRKGA"
     gene            10547..11434
                     /locus_tag="ACIYSB_004334"
     CDS             10547..11434
                     /locus_tag="ACIYSB_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5431249.1"
                     /translation="MKMSIKQLRAFLAVAHTLNFAHASERLNMSQPALSLAIKGLEDA
                     LGGALLLRTTRKVTLTQEGETLLSMARPLLADWDNTEEIMRQRFTLRRGKVSVAAMPS
                     FAANVLPTVLKTFRDCYSGINVTVHDVVNEQVIEMVSEGRVEMGIAFEPDNSDRFHFT
                     ALGVDRFVAIVPADSRLANRKHVSWRDLLTLDFITLQRPSAVRFMLEEQLAQSGMQLE
                     VAMESHQLVTVGRLVANGLGGSAVPALCRRQMGEQGAVCLELDNPVIEKRVGVLRSPH
                     HKLSTAAQALMNALKEYYG"
     gene            12077..12412
                     /locus_tag="ACIYSB_004335"
     CDS             12077..12412
                     /locus_tag="ACIYSB_004335"
                     /inference="COORDINATES: protein motif:HMM:NF015301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HGC5431250.1"
                     /translation="MPLGIYHSRVAGIYDTPGSMSDAHFEILAALSPVNSKKVILALR
                     DYFVLGLTRREACENHDVAQGYFSVSLRKLLETNRLVAELAVYYQPSHQAHQENENRE
                     EAHHRQYYR"
     gene            complement(12489..12632)
                     /locus_tag="ACIYSB_004336"
     CDS             complement(12489..12632)
                     /locus_tag="ACIYSB_004336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431251.1"
                     /translation="MNQGMHNASQCCSGRGIAGLSRAFLNKKVRSCPDTSPASPSHGV
                     QKL"
     gene            12615..12701
                     /locus_tag="ACIYSB_004337"
     CDS             12615..12701
                     /locus_tag="ACIYSB_004337"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431252.1"
                     /translation="MHSLVHQVSRFGIRINTAAPGAYGIMFW"
     gene            complement(13998..14351)
                     /gene="yebF"
                     /locus_tag="ACIYSB_004338"
     CDS             complement(13998..14351)
                     /gene="yebF"
                     /locus_tag="ACIYSB_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGC5431253.1"
                     /translation="MNKALSSILLIFGICASSLAAEKNRTVSFPRCEVQEASAVAASV
                     KRDYLQKQVFQWKDDQEILGQQRPVVWVNLREPVNKGDNVITVHLTARGKKADIQYRV
                     RVDCKNGSATWQRKG"
BASE COUNT         3754 a         3478 c         3612 g         3721 t
ORIGIN      
        1 atagtggata aactgcccct gaaaatacac aagtacgcca ttttttcctt gtgtgataag
       61 caataaagtc aacgaataac gttgtactaa tgaatatgcg ccttgctgaa cctggctttc
      121 gccactgatg aatagcaact cttcttcgga caatttagcg atattcgcca gcgaaagtgc
      181 gcgatcaagg tatttcctta atagcgcttc actctgccag agatcgctgc ggatattggg
      241 atcaaaactg atgtttccgc cagcctgacg tattttctcc atagccagaa atgcggtact
      301 tcgggaaggt tctgcactga gcgcaatgga acaaagatgg agccattcac ccggtccaaa
      361 tgcaggaagg tcatcaggtt gtaaaaaaag atcggcactt gggcgtacca taaaggtaaa
      421 gtttctttct ccttgctcat caagtcctac agccacaatt gaggtgcgat gttgaggatc
      481 aagagacata taacgtgtat caacattttc tgtactcagt gtctgataca taaagcgacc
      541 gaaaggatca tcgccaactt tgccaataaa ggcacttttc ccccccagcc ttgcgatacc
      601 gactgcaaca ttagcaggcg ccccgccagg acactgtagt agactcccct ggctttcggg
      661 taataaatca acaaccgcat caccgagtac ccatactctt gtgctcataa aggctccaat
      721 gtactaattg ccaaaccaat taaatcgttt tagcaattaa agcataaacg ctgctcagga
      781 gagaacaata ttcgtaaacg caaactgact tctgtatgta cccggtactg gtaatgaagg
      841 tgaacacttt tcaattagtg gataaatgta taggataaaa agcattattt gcagcacctc
      901 gtaggtcatt tttaaatgag cttaaacttc agatggttaa accggtatta caaccatgag
      961 tatctgttgt ttgtatcgcc tgggacacga tatcaatgaa cgtcaataca gagttctatt
     1021 aacgcttcca ctgcccaggg agaagaaagt tgaaaattac gaggttgtga tgtgatctta
     1081 tcttatcgtc ctgaataaag ctctgttgag acaggttatt gatgcgattg cgaaaatcaa
     1141 tcacagcttt aaaagtattc aacctgaggt gctcaggata acgggggaac tattgttcaa
     1201 tgcggtgtcg aacagtgtct gttatgaacc gacatccaga ggatgtcggt ctgtcaacgg
     1261 aggattaatg caactcaggc cagtaatctc tgttggcggc aatcaggtcg tccaggatcg
     1321 ctttcgccac cgaggcgctg ggcacggttt tcgacagagt tatcgcctgc cacagcttgt
     1381 gataagaacg ctgctcccat gcatcgacca ccagtttttc caccgccacc tgttggctca
     1441 tcaacccttt ctggaagagt gggatgttgc ctactgtcag cggctccggt ccgttatggc
     1501 ccaccagaca agggatttcc accatcgcgt cggcgtcaaa gttatggatc gcgccattgt
     1561 tcggcacaat cagtaacatc ctttcctgag tgttaaaggc gatggccgtt gccagatcga
     1621 caatatagga tgcatgctcg tcaatttcca gatcgccggc ggtggatttt ccggcggcga
     1681 tgatagcacg acaggcgctg aagacgtttt tctcccgatg atccattacc tcattggcgc
     1741 gggtgcgttc cgggttcgag tgggccacca cgtagtctgg gaaaaggtaa tatttcaggt
     1801 atgtattcgg catggtttgc ggatccagag cctgaacgtc tttcgctttg gcaaaggtat
     1861 cgttccagct ggcctcggtg tgtggatcgt tagagggcgg cacataaccg gattttgcta
     1921 catattcgcg cagtttcggc atcaaatcat tgccgtccag gtcctcaatc gaggtccacc
     1981 agccgaaatg gttgaggcca tagtagcgca cgcgcatctg tttacgatct ttgaggccta
     2041 caatttgcgc catcctccct tcaatgccga tcggcatatc gcagatgttg agtattctgg
     2101 cgttcggtcg cagacgacga gtggcttccg ccacaatcgc tgccgggtta gagtagttga
     2161 gcatccaggc gttcggtgaa tatttttcca tataatccac cagttcgagc actcctccaa
     2221 tagaacgcat accgtaggcg atcccaccag gtccacaggt ctcctgaccc agtacaccgt
     2281 gtcgtagtgg gattttctca tcctgttcac gcatcggata tttaccgacg cggatatgcg
     2341 ccataacgaa atcaacatcg gtaaacgcta cctgcggatc ggttgtatag cagaattgaa
     2401 tatccggtgc ttgctctttg aggagcactt tgcacgcctc ggcgatgatc tcctgtctcg
     2461 ctgggtcatt atcgtaaaat ttcagcgacc gcagcggaaa acgatcctgg tttgccagca
     2521 gcatcaggac aatgcccggg gtaaaggtgc tgccgccgcc tgcgataaca actgagaatt
     2581 ttttcatgat acagcctccg tcatagtcgt gtgtttggtc gaaagagaat ccttcatcag
     2641 gttttccacc tggtcgcgca cctggggaac gtgcaggccg acgatgacct gaatcccgtt
     2701 gccgcggcgt actactccgt gggcgcccag tgctttaaaa acatcgtcac tttgtgtttt
     2761 cgccatgtcg atcagcgcga tgcgcagtct ggtggcacag ttgttgatac tttcaatatt
     2821 gtcagcgcca ccgagagcct gaaggaaacc agcggcctgt cgcagacagg gatctggctc
     2881 tatagctgta gttttccctc gtgcagcctg ataatctgcc ttgctgtaga gctttatttc
     2941 actcgattcc cggcctggag tcttcaggtt cagacgcagg atcagtgtgc ggaagaccac
     3001 aaagtaaagg acggtgaaac agagaccaat gccagtctga atgaacatca tagaggaatg
     3061 atgatggaac atagggatcc agttttgcgg taagaactgg tcgagcaggc cgccaccgaa
     3121 attgcccacc acgccgcata tgtacattac cgttgccatg gtcgctgcca gcaccgcgtg
     3181 aacggcgaac agaagcggtg atatgaacag aaaggtgaac tccagaggct ctgtgatccc
     3241 gaccagcatt gctgttaacg tggcagggat cagcaggcca gcgactttta cacggttttc
     3301 cggcgtggcg gtgaagtaga gtgccagagc aattcctgcc gaaccaaata ctttcgagtt
     3361 accgtgcagg gcaaagccgc cctcagggaa gagtgtctta agtggttccg tactctgact
     3421 gaacgcctgc agatgctccg cccagtagac ctgcagacct ccctccacga ctgccgggcc
     3481 aaagatgaac ggaccataga caaaatggtg caggccagtg gggatcagga tacgctcaag
     3541 gaaggtgtag acccatacac caagcgcacc ggctgagcgc aggaaggcct gaagtgattc
     3601 aatgtacagt tgaacttttg gccagccgag aagagttaac caggcacagg gtagcatcgc
     3661 gaggaaagcg acaatgacca caaacgatga gccctggaag atgccgagga ataccggcag
     3721 gggtttatcg aaatatcgat tatgcaaagc agtcaccagt ccagagatga cgatcgcccc
     3781 gataatgctg gtgtcgagtg ttttgatccc ggcaatcatc gtcaggccac tgccggctgt
     3841 gggctcaatg gaaaaatcga ctccgaagaa gtggccccag gtcgtcccca tggcgttgat
     3901 aaagtagttc catgtgagaa aactcaccag tactgccaga caggccctgc cctgtgcctg
     3961 tttcgccagg ccgatcggca gaccgacggc gaaaattagt ggcatattac gaaagacggt
     4021 ccaaccgccc tcttcaatga tgtgaacgat ttgtgcaaac aggctgtcgg gagcggtcag
     4081 tgcttcaccg acaaacatcg ggttgcgcag catgatggaa ataccgacca cgatcccggc
     4141 aaaaggaaac aacaaaaccg gggtaaacat ggcgccgccg aagcgttgta tttgactgag
     4201 catgtgaaaa tccttatgta gcaactcgta gggtgagtgg atttcttctt cttaattacc
     4261 gacttcattc gagaatagaa atttgcctat tcgacaacac gtagtactgc tgaaatttgt
     4321 gagccctttc atggttttaa tttgaccaaa gttgtctact tttattaaag aagtcagata
     4381 tctataaatt acagagagat atcggccaaa tgaaagaatg gtaattaact gtatttaaaa
     4441 attattctct tatcttttgt gaaagaatga acatcctgtt ttttgtacga gagggctatt
     4501 ggtgatctat aaatctattg ctgaccgcct gagactacgg ttaaattcca tcgacttcgc
     4561 catcggaagc ccacttccgg gagaaaaaaa actggcttca gagttcggag tggcgcggat
     4621 gaccatccgt aaagcagttg atcttctggt cgacgatggt ctggttgtgc gtcgccatgg
     4681 ctcgggaaca tacgtggcac gtaaagacat tcatcatgaa accagttctc ttactggtct
     4741 ggctgaggtg ctgcgcaagc agggaaaaga ggttgttagc agggttattg cgttcgaatt
     4801 gatgccagcc ccgccagcta ttgccagcat tctgcggata aaaattgatg aaagaatttt
     4861 tttctcacag cggatacgtt atgtggacga caaacctctg atgctggagg acagctatat
     4921 gccactgaag ctgttccgta atttgtcact gagtcatctg gagggatcta aatttgatta
     4981 tattgagaag gaatgcggga tcatcattag tgggaattat gaaaccctga cgccggtgct
     5041 ggcggataca caacttgccg gcttaatgaa cgttccggaa gagacaccac tgctacgtat
     5101 tacatcgctt tcttacagcg acaacggcga atttctcaac tattccgtaa tgtttcgcaa
     5161 tgccagcgaa tatcaggttg attatcactt gcgacgtgta caggcacgaa gctcatcaga
     5221 tcaggaaccg gaatagaaaa ggggggtatg tgcgtgagca ataacaaatt tttgccggat
     5281 gaccagtcat ccggcaacgt tttactgcga aatccagccc ccatccatat tccatgctgc
     5341 tccacgtact tgtgctgctg cgtccgaaca gagaaacagg gacagttcgc ccagttgctc
     5401 tggagtcaca aattcacgag acggctgttt ttctgccagt aaggcgttat gtgcgctttc
     5461 ttcatcttcg ccttgctcaa tgcgtttatc aatctgttgt tgtaccaggg gagtcagcac
     5521 ccagccaggg cagagcgcat tgcaggtaat acctgtctga gcagtttcca gagcaatggt
     5581 ctttgtgagt cccaccacac catgttttgc tgcaacataa gccgatttgt ctttcgacgc
     5641 taccagccca tgcacagagg caatgttaat gatccgcccc cagttacgag cccgcatgtc
     5701 cggcagcgcc agacgagtgg tgtgaaagac agaagagaga ttaatggcga taatggcgtt
     5761 ccatttttct accgggaaag attccacagg ggagacatac tggatcccgg cgttattaac
     5821 cagaatgtcg acaccgccaa attcctgcca tgcgtaggcc atcatactga caatttgcga
     5881 ttcatcgctg aggtctgccc catgatagcc cggtgtttta cccacacgag aaaccacatt
     5941 gagtgcctcg ttaacatcac cgaaaccgtt gaggatcact tgcgcgccag cttttgccag
     6001 cgtctgagca ataccgagtc cgataccact ggtggatccc gttaccagcg cagttttacc
     6061 ttgtaaattc atgattcttt cctcagacaa tgccggtggt gtagtacgcg ccgatgataa
     6121 acagtacggc cagactttta atgatcgtaa ttgcgaagat accaccgtaa gcctgacggt
     6181 gagtcagccc ggtgattgcc agcagagtga tcactgcgcc gttatggggc aatgtatcca
     6241 taccaccact ggccatagat gccacacggt gaaaaacttc cagtggaata tgcgctgcat
     6301 gtgcagcggc aataaaggta tctgacatag cagcaagcgc aatactcatt ccgcctgaag
     6361 cagaaccggt tataccggca agcgtagtga tactgatagc ttcgttgagt aatggatccg
     6421 gaatggctgc cagtgcttcc tggagtacca gaaaaccggg aagagcagcg atgacggctc
     6481 cgaaaccata ttcagatgca gtgttcatcg ccgccagaat ggcaccgccc acagctgtac
     6541 ggctcccttc cgccagcttt ccatgaatat ttctgtaacc aaaagataca acgatcacaa
     6601 tgcctgcaat gagtgcagct tctacggacc agatagcggt aattttagca acatcagtag
     6661 ttatcggtgc agtcagacca ggcaacacca gttgatgact tgtgccgtac caggtgggga
     6721 tccaacgggt aaacaacagg ttcaacagac caaccagaat tagtggtgat atcgcgagga
     6781 gtggattcgg taaattgatg ttgtctggtg tttcaggttc attaagtaac tctgtgccat
     6841 aaccttcatt ttttacctgc gctttatgac gctgccgctc cagatagata agcccaacga
     6901 caatgatgaa cagtgaacca ataagaccca tccacggtgc ggcccaggca ttagtaccaa
     6961 agaaactggt gggaattata ttctgaattt gtggggtacc gggcagcgcg tccatagtga
     7021 aggagaatgc cccgagagct attgtggctg gtataagccg ctttggaata ttactctggc
     7081 ggaacagttc agctgcgaac ggataaacgg caaatgccac tacaaacagc gacacgccgc
     7141 cataggtgag taatgcacat accagcacaa tcaccggaat tgcatgccgc cgtccgagaa
     7201 tgttgatcgc agcagcgaca atcgaacgag aaaatcctga aagttcaatc agttttccga
     7261 acacggcccc gagcagaaat acgggaaagt acaatttcac gaaaccgacc attttttcca
     7321 tgaacaggcc ggtaaacgcc gggccaacag cttccggatc tgtgataaga actgcaccta
     7381 acgccgctat gggggcaaaa aggataacac tgtatccacg gtaggcagcc agcattaaca
     7441 gtccaagcgc tgccagggtg attaatacac tcattgggac acctcaaatc gttgttatac
     7501 cggggacagc gagagaagtt atggattctg tgtgcaactg atttgccctg ctttgccgga
     7561 tatcagtgca cgccgctgtt ttcattctta acgtgtcagg taaccgacgc tatgacgttg
     7621 cagcagttct ccggtcacca gaaattctgc ctgagataaa agggactcct gtgctcccgt
     7681 tccagccggt attgaaaatt ccggacagaa atataatgag tggttgtaat cctcacgttc
     7741 acctggctgg ctcagattcc gaacaaacag cctctgacct tgtggaaaac cagtattcat
     7801 gcgggttcag tactacgaat ttttcagtat acattgcatt ctccggtggt tctttccagt
     7861 gccagtgcca cgccctgacc gccgccgacg cacagcgttg ccagcccttt ttgggcatta
     7921 cgtcgtgcca tttcataaat gagagtgacc agaatgcggc aaccggaagc gccaatagga
     7981 tgcccaagtg caattgcgcc gccgttaaca ttaactttgt cgctatccca gttcagttcg
     8041 tcaccaacgg caagcgcttg tgcagcgaaa gcctcgttag cttcaataag atcaatgtca
     8101 tccagtgtcc atccgcaacg tttaagacat tctctggtag cggagaccgg accgatgccc
     8161 ataagtgcag gatcgacgcc agaaacagca tatcctgcca cccgagccag cgcagggagc
     8221 cccagttctg cagctttgct ggcgcgcatt aacatgactg cggcggcacc atcattgatg
     8281 gaagaggaat tacctgcggt gacgcttccc gcccccggaa gaaaggccgg tttcagttgt
     8341 gccagttttt ccagttgcgt cccggaacgt ggttgttcgt cggtatttac ctggcgtggt
     8401 ggatttttac cttgtggtac gttgaccggt gcaatttcgt ctgcaaagcg tccggtttcg
     8461 attgctgaag ctgctttttg ctgagaatgt agcgcgaatg tgtcctggcg ctctcggctg
     8521 atggcatatt tttgcgccag gttttctgca gtaatgccca tgtggtaatc attgaaagca
     8581 tcccatagtc cgtcatgaac cagactgtct ttcatactgg tatgcccgag gcgtatacca
     8641 gcccgagcgc cattaataaa ataaggggca ttactcatac tttcctgtcc accagcaata
     8701 atgatctgtg catcatcaca acgtattgcc tgtacggcct gaattacggc tttcagtcct
     8761 gaaccacaga cctggttgat ggtgacggcg ggagtatggt tgcttaatcc agccagaaga
     8821 gccgtctggc gggccgggtt ttgcccacac cctgcggtca gaacctgccc caaaatcact
     8881 tcgtctatga gttctggtgc aatagcagtt ttctccagca atgcccggat ggcggtagcg
     8941 ccgagatcaa ctgcagaaag tggtgcaaga gagcaaagaa agctgccaat tggcgtacgt
     9001 gtggcggcaa caataacaac gtcctgcatg gtatctcctc agttgaatcg catttccggg
     9061 acatgttctg gcacaaataa cttaccggcg gttctggtga cgatttcctc aatgctgact
     9121 cccggagctc gctcgcgaag aatgaatgtt ccatcagcga tatccagcag agcaaggtca
     9181 gttaagacac gtttgataca gccgacgcca gtcaacggca aggtacagtt gggaagcagt
     9241 ttggattcgc cggatttgga ggcgtgggtc attaccacaa taatattctc ggcaccggca
     9301 accagatcca tggcaccgcc catgccttta accatcttgc cggggatcat ccatgaggca
     9361 atgttgccat tcacatccac ttcaaatgcg ccaagcacag tcagatccac atgaccacca
     9421 cggatcatgg cgaacgattg cgccgaatcg aaaattgccg cgcctttccg cgcggtaacg
     9481 gtttgtttac ctgcattaat catgtcggcg tccagttctt cttccattgg aaatgcgccc
     9541 attcccagca aaccgttttc tgagtgcaac atcacatcca tcccgtcagg aatgtagttt
     9601 gccaccagag tagggatacc aatgccgaga ttaacgtaat agccatcgcg caattcgcgg
     9661 gcgacacgca tagccatttg ttcacgggtt aacatggtca ggctccttta cggctaagag
     9721 tgcgttgttc aatacgtttt tcaaattttc cctgaatgac acgattgaca aaaattccag
     9781 gggtatggat agtggcaggc tcaagttcac caggtggcac aatttcttcc acttccacca
     9841 ctgtgatttt tccggctgca gccatcagtg ggttgaagtt ttgcgcggta tgacgataaa
     9901 tcacgttgcc gtaccagtcg gctttccagc ctttcacaag ggcaaaatct ccagtgatag
     9961 cctgttccat gatgtacggg cgaccgttga attcgcggac ctctttccct tcggcaacgg
    10021 gagtaccgta accggtggca gtaaagaagg ccggaatgcc cgcgccgcct gcgcgcagtt
    10081 gttctgccag ggttccttgt ggtgtgagaa tcacttccag ttcaccgtta agtacctgtt
    10141 tttcaaataa agcattttct ccaacataag aagcgacgac tttacggatt tgtcgggttt
    10201 ccagcagcac acctagtcca aaaccatcta caccacagtt attggaaacc accgttaatc
    10261 cggttactcc gcggtggcta acttctgcaa tcagattctc cgggatgccg cacaggccaa
    10321 aacctcccgc cagaattgtc atctcactgg taagtccttc aagcgcctca tgataagtag
    10381 cgacccgttt atccagtcca gccatacagt tgccctccag ttgtcctttc caacatcatt
    10441 aagggcaaaa aatgatttgt taattttaaa tttgtgattc attcatttgt tttgtttatt
    10501 gatctggtgt gaattaaatg ttttcaaaaa gttaattgga tgctgtatga aaatgagtat
    10561 taagcagtta cgtgcgtttt tagcggtagc tcacactctg aactttgctc atgccagtga
    10621 gcggctgaat atgtcacagc ctgcgctgag tctagccatt aaaggactgg aagatgcttt
    10681 agggggcgct ttactgttgc gtactacgcg aaaagtgacg ttaacccagg agggggaaac
    10741 actattgtca atggctaggc cgttgctggc tgactgggat aacaccgaag agatcatgcg
    10801 tcagcgattc acgctcaggc gaggtaaagt ctcggtggca gcgatgccgt catttgcagc
    10861 caacgttctg cctacggtgc tgaaaacctt ccgcgattgt tattcaggga ttaatgtcac
    10921 cgtgcacgat gtggtgaatg agcaggtgat cgaaatggtg agtgaagggc gcgtggagat
    10981 ggggattgcc tttgaacctg acaatagtga ccgttttcat tttacagcat tgggagtcga
    11041 tcgatttgtc gctattgttc ctgctgattc ccgactggca aatcggaagc acgtgagctg
    11101 gcgggatttg ctgacgcttg attttatcac tcttcagcgg ccgtcagccg tcaggtttat
    11161 gctggaagag cagttggcac aaagtggaat gcaactggaa gtcgcaatgg aaagccatca
    11221 acttgttacg gttggacggc tggtggcaaa tggtttggga ggaagtgctg tgccggcact
    11281 gtgtcgcagg cagatggggg aacagggcgc agtgtgtctg gagctggata atcctgtgat
    11341 tgagaaacgg gtaggtgtac tgcgttcgcc acaccataaa ctttctactg cggcgcaagc
    11401 actgatgaac gcattaaaag aatattatgg ttaatctttc catccaacgg cgggtgcaca
    11461 gaaatgttaa aaaatatgtg tgcaaatgac ggtaatgtgt taaaagaaca ttgcgcgttt
    11521 ccggtacgct cttcgtaact aacaatgcag ggtgacgtaa cgctggttgt gccggagacg
    11581 cgtgttgtcc tttttatctt tgcctcctgt ctttttcttt tttatttaat atcattattt
    11641 ttattaatta tacctgaata aacatgaagc ctctccgtga tataacagta cggtatgaag
    11701 gtgtctcacc gtgcttattt acggaagccg ggggaagata tcagcgcaag agtgtcacga
    11761 cgttttttca ggttttaacg taaaaatgtt attttattta ttcccgcctg tatgacctca
    11821 tccacagaat gataaaaacg catcaaaaac cacacatatt ttacttatta ttgttttggc
    11881 agcattaaca tctgccccgc acattcactc tgagattaaa tgtcatgatg ccggcattat
    11941 acagaccggt gtctgtgctg aagtcatggt gccgtgttga tgtaacggaa tgttttcagt
    12001 atttttaaaa taaatattat ctgttaaata tttaatttat taccaccatg acgggccgtc
    12061 agcgttattc agggaggtgc ctttggggat atatcacagt cgtgtcgccg ggatatatga
    12121 cacgcccgga agcatgtcag atgcacattt tgaaatactt gccgcgcttt ctcccgttaa
    12181 cagtaaaaag gttatcctgg cgctgaggga ttattttgtt ctgggtctga cgcgcaggga
    12241 agcctgtgaa aatcatgatg tggcacaggg gtatttttca gtgagtctgc ggaaattact
    12301 ggaaaccaac cgccttgtgg cggagctggc tgtttattat cagccgtcac atcaggcgca
    12361 tcaggagaat gaaaacagag aggaggcaca tcaccggcag tattaccggt aatcaggcca
    12421 tttacagtaa acagtggcag caccggagac tggtacagaa cagatgtgag tggctggctg
    12481 gggattagtt acaatttctg aacgccgtga gaaggggaag ccggggaggt gtccgggcag
    12541 gaacggactt ttttgttcag gaaagcccgg gacagaccgg ctatgccgcg accagagcag
    12601 cactgactgg cattatgcat tccctggttc atcaggtgtc ccgtttcggg atcagaatca
    12661 atacggcagc gccgggggct tatggaataa tgttctggtg aaatcgtcct gacgcgtttc
    12721 tgggctcgtc gtcagtgatt tatgcgaatg attgccgccc tgcgatggtt gatttatgag
    12781 taatcatgct cagccacagt gaagaacagc accagtcatg gtatgatttc tcccataaaa
    12841 aacagccgac attctcacaa tgccggccaa atcttatatg caaatattaa ccatgcagtt
    12901 acagggggta accgcaggaa catgctaagg tccggtaaca gtgacggcaa gcgcgactga
    12961 cttatttaac gtatcaaaat ttgctcctga tcattgagag gacataccgg gataaatcct
    13021 gatcgatcct gctctaacat cacggtattt ctgcaggact gtgcccgttc gcgagatgat
    13081 atacagtgac ggaatgaata attcagggct ttattaccgg aatgaagatg tgttcaggat
    13141 gatgccatca catccgtcag atgcccgatt agcatcatga ccataaagct ggaatacagc
    13201 gcgtttccga cagtgacatc aacataaact atcagtaacg ttaattgcat cagaaacgcg
    13261 cgggaggatt ttgcccctgt gcataacgac ttgtccatgg gaaatacata aatttaataa
    13321 aaaagaaaaa tgtggttgtg aatattataa tatcccgggc aacgctcctg atttttctca
    13381 atacacattt tcctcacgta tttgttcttc acagagcaat cagcatctgt tactgcctgg
    13441 cgaatatgag tattaaaaaa gagtaagcca aagaaagaat aatggggagt tgttgaactg
    13501 gagggatgtt gcagatgctg cgtgttgaat catacaaagg gccgacatct tcatggtgtc
    13561 ggccagtcat aagtgccaga tattaaccgt cagttacagg gggtaaccgc aggagacagg
    13621 ctacgctccg ggtaacaggt tatcaatagt tatttatttg cttaatttaa tgaattttgt
    13681 tctgcatcat tgcgctttct tactggtata aataatgatc gatcgctttt cgcatgagca
    13741 tcagaatttg tcgtaatttg aggcatggat gctgtacata caggaatatg ttctgtcagc
    13801 gacagattgc cggtgctgat cggaatgttc aggcaggatg gtgagattac tactgatatt
    13861 gctgtgctga tggcagaatg gtaaacgtta aatgcgcgtt cccggcaatg aaggcaaaca
    13921 cgtcataaca gcattcagtt atatacgctg agtatggaga gcccgttcgt tcgggctctg
    13981 tatttagctt cattcgttca tcccttgcgt tgccaggtgg cacttccgtt cttgcagtca
    14041 acgcgcacac gatactgaat atctgctttc tttccacggg cagtgagatg cactgtgatg
    14101 acattatcac ctttatttac cggctcacga agattcaccc agacaacggg acgctgctga
    14161 ccgagaattt cctgatcatc tttccactga aatacctgtt tctggagata gtcccgtttg
    14221 acgctggctg caacagctga tgcttcctgt acttcacatc gtggaaaact gacggttctg
    14281 tttttttcag cggccagtga tgatgcacag ataccgaaga tgagcagaat ggaagagagt
    14341 gctttgttca tttacaggac ctccacaggg gatagtttca ttgcggagtt acattttaac
    14401 aacgtaaaac agcatttccc gtaacaatcc tcacataacg tcttatcccg tcacttcccg
    14461 tttcacatgg acagaacagt ctgtttttta acccgttttt tcagtcattt ccataaacaa
    14521 atcttcgctg atgataaatc ttttttctgc ttaacgatcg atttt
//
LOCUS       DBBGLQ010000112        14222 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.112, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000112 DBBGLQ010000000
VERSION     DBBGLQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.112"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            36..641
                     /locus_tag="ACIYSB_004339"
     CDS             36..641
                     /locus_tag="ACIYSB_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGC5431254.1"
                     /translation="MSNKELITEFLLAAEQGNSDGLKSCLDKKVDINATNREGRTAII
                     IASLNKHYDCVSLLISAGADINKQDQTCFNPFLIGCLNNDLTLLRTVLPANPDLNCLT
                     RFGGVGITPACEKGHVEIVRELLMRTDINVNHTNFVGWTPLLEAIVLNDGGEKQQEIV
                     KLLLEHGANPHMTDKYGKTPLELAREKGYHKIAELLISAGA"
     gene            727..1575
                     /locus_tag="ACIYSB_004340"
     CDS             727..1575
                     /locus_tag="ACIYSB_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGC5431255.1"
                     /translation="MGQVEQVFSHAVNLLIPEQQRLLTLLSEDCDNAPNSCRLALTHY
                     EDLFHPGEQVNFSGSGITVGNDKWIEITGCQMWRIPRWTSDSASLAKVGWQHWEEFIR
                     QQLRSNDTLFLYQGDNPFYQEISRQLLNRRKNLLKALCTGENITAAVNQLIGLGIGLT
                     PSSDDYLVGLSTILFIQGHPLRKYRYDFLAALQLAGNNITLLSKITLEEAFNQRYRER
                     VGRLLNQFITEPQPISTQYVTDIKNIGSSSGCDMLYGMADACALSHRSGGNYVNQDSC
                     KKKYVF"
     gene            1535..3082
                     /gene="fdrA"
                     /locus_tag="ACIYSB_004341"
     CDS             1535..3082
                     /gene="fdrA"
                     /locus_tag="ACIYSB_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGC5431256.1"
                     /translation="MSIRIVVKKNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEEAKSGDLMIVIKGTSEEANERTLIAIDELFAHKEQGGQHEARYA
                     TIASAKKHAPESNLAVISVNGLFAAREARQALQNNLNVMLFSDNVSLEDELELKHLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIIGASGTGSQELSVRIHEFGGGISQ
                     LIGTGGRDMSEKIGGLMMLDAISMLEADPQTEIITLISKPPAPAVARKVLERARTCHK
                     PVVVCFLGRESTPADEEGLQFARGTKEAALKAVLLTGVKKESLDLHPLNWQLIEEVRS
                     RLTPQQKYIRGLFCGGTLCDEAMFTAMEKLSDVYSNIHPNPAFRLTDLNRSVAHTFLD
                     FGDDYFTNGKPHPMIDPTNRITRLLQEAQDPEVGVIVMDFILGFGSHEDPVGVMLDTI
                     VEAKEIAAADGRPLEILGYVLGTDLDTPSLKKQCQMLTDAGVIWASSSTNTGLLAREF
                     IFKGEEG"
     gene            3082..4500
                     /locus_tag="ACIYSB_004342"
     CDS             3082..4500
                     /locus_tag="ACIYSB_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGC5431257.1"
                     /translation="MSQTLFNQPLNVINVGIEMFSDDMKKQNVPVTQLDWTPPGQGNI
                     QVVTALDDIAASPLADKIAAANQLALERIIQSHPVLIGYDQAINVVPGMTRNTILHAG
                     PPIHWEKMCGAMKGAVTGALVFEGLAKDLDDAAELAASGEIIFSPCHEHDCVGSMAGV
                     TSASMFMHIVENKTYGNRAYTNMSEQMAKILRMGANDQSVIERLNWMRDVLGPMLRDA
                     MKLAGEIDLHLMLAQALHMGDECHNRNNAGTALLIQTLTPWIIQTGYPVEQQREVFEF
                     VLSSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRISGLPGKWFTGPA
                     QQVIGPMFAGYKLEDSGRDIGDSAITETYGVGGFAMATAPAIVSLVGGTVEEAIDFSR
                     KMREITLGENPNVTIPLLGFMGVPTAIDITRVGSTGILPVINTAIAHKDAGIGMIGAG
                     IVHPPFTCFEKAILNWRDHYCQ"
     gene            4613..6163
                     /locus_tag="ACIYSB_004343"
     CDS             4613..6163
                     /locus_tag="ACIYSB_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HGC5431258.1"
                     /translation="MNSIKLEWKRGDWAAYFGLMTNNLTNLLTMMGLLIFVVGIPTEI
                     VYGRIAPAFGLAVLAASVCYSWFGLQMVKKTGRSDVTALPSGPSAPSIFTVTFLVLMP
                     VYQQTKDANFAIQIGLVWCFVEALILVGGSFLGETIRKMIPRTVLLSCLSGLGLLLLA
                     MNPMLQAFEAPTVSFIVLLLIFINWFGKKPIFPHIPTGLLLLIAGTSLAWISGLQSPE
                     DIKASMSSFGFNPPEIHVRSFLQGLPHALPYLASAVPLGLANYIFDLENIESAHAAGD
                     EYNTRKVMMANGFASMLGCMLGNPFPVTVYVGHAGWKAMGASICYTLASGITMFLVPL
                     FGLGAFMLAIIPMTAIVPILVFIGVVTANQVVRETPKVEVPVIFICLFPWVANWALTI
                     VNSVMGAAGTSAEKLGSDLLHSKGVYYDGLMHLGSGAPLASMLWGCIAIFAIMNKPLR
                     GAIAASFGALLSLFGVIHSPAVGFAEGSSIVFVVAYLMMLAMFALKHVIDSRETVAAS
                     EQEETKTT"
     gene            6190..7152
                     /locus_tag="ACIYSB_004344"
     CDS             6190..7152
                     /locus_tag="ACIYSB_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGC5431259.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     PHGEKKAVTVVTQVEVDKNDPGFVHPTKPIGAFFSENQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKTLIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     SSALLAREIHADILVITTGVEKVSIHFGKPNQHALDTVDVLTMARYMQEGHFPPGSML
                     PKILASLEFLERGGKRVIITTPECLSAALRGETGTHIIHSQEET"
     gene            complement(7988..9260)
                     /locus_tag="ACIYSB_004345"
                     /pseudo
     CDS             complement(7988..9260)
                     /locus_tag="ACIYSB_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9782..11182
                     /locus_tag="ACIYSB_004346"
     CDS             9782..11182
                     /locus_tag="ACIYSB_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5431260.1"
                     /translation="MNTHHPSSSVPLVDNQQAAQERLETPEGRREFWRATFSCWLGTT
                     MEYADFALYGLAAGIIFGDVFFPEVTPVIALLSSFAAYSVGFIARPIGALLFGWIGDK
                     HGRKIVMVITIGLMGMSTMLIGLIPSYAQIGVWAPICLVILRFSQGLGAGAELSGGTV
                     MLGEYAPVKRRGLVSSVIGLGSNSGTLLASLVWLIVLQMDKDDLLNWGWRIPFLCSIL
                     IAAAALLIRRHIRETPVFERQKALLQAEREKVLREGKAQQQHDTRSFWKRTRAFWIMV
                     GLRIGENGPSYLAQGFIIGYVAKVLMVDESVPTAAVLIASVLGFAIIPLAGWLSDRFG
                     RRIIYRWFCLLLILYAFPAFMLLDSREPWIVIPTIITGMGLASLGIFGVQAAWGVELF
                     GVTNRYTKMAFAKELGSIMSGGTAPLIASALLSFYGHWWPIAIYFAFMAAIGLVTTFF
                     APETRGRDLNLPEDAI"
     gene            11287..12318
                     /locus_tag="ACIYSB_004347"
     CDS             11287..12318
                     /locus_tag="ACIYSB_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGC5431261.1"
                     /translation="MTQSHSQRVTRSDVAKEAGTSVAVVSYVINNGPRPVAEATRQRV
                     LQAIKKTGYRPNGIARALASGSTQTYGLVVPDISNPFIASIAHALQHEAFADGKVLLL
                     GDAGDSSCRERELINNMLHRQVDGLIYTSVDRHPYINLIQESGTPCVMLDRVDPGLNV
                     SVIQVDEQLAAMLVTQHLIDHGYRDIGIICGPREMLNTQDRIRGWRQALEASSLVVNP
                     SWIFSTNYTRAGGYEATKRMLENQLPRALFATNEQQALGCLRALAEHGLRVPEDVALV
                     CFNATQESVYNVPSLTAVRQPVDKMARAAIDMLKNWDGEVRRVEFEFYLRTGESCGCQ
                     GHEVQPEKK"
     gene            12293..13312
                     /locus_tag="ACIYSB_004348"
     CDS             12293..13312
                     /locus_tag="ACIYSB_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HGC5431262.1"
                     /translation="MKYNQRKSENESMRIIIDCDPGNGIPGANIDDGLALALAIAAPQ
                     IALEMITTVAGNTPVDVGYAVARDLITQLDIPVAVYRGASRALREDPQPWREKLDHGV
                     DQFGLRQLWTNVPAPALYQKVEPHAPEAIGELICRNPGEITLVATGPLTNVAIALQLY
                     PQIVHAVKNIVVMGGVFNVPGYLKDTNFGLDPEAAHAVLTSGAPVTLVPMDVTTQTQM
                     LHADLERLAKTENGLSRYLAQTIRPWITYSMQTRNLPGCWIHDVLTVAWLLDPSLATT
                     AEDYLDVSLEGITRGMTCCYGRDTLRLNVGIPEPKGARVTILQSIDNPRLISLIEHYI
                     QSYGA"
     gene            13376..14089
                     /locus_tag="ACIYSB_004349"
     CDS             13376..14089
                     /locus_tag="ACIYSB_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGC5431263.1"
                     /translation="MNTLSFLFFGEGESLLITLGKICVAFILGGIIGLERESKGKPVG
                     FKTCVIISVASCVLTIVSIQSAEYYAEISMNIRSDPMRLAAQIISGVGFLGAGVILHR
                     HDDAISGLTTAAIVWASAGVGITCGAGFYMHALVTTALFMIAIKLSYFVLFLQAKNQF
                     PGKVKVHVTLDERSGLQALIECINQQKNIIEAFTIRDVKKGRIEVNLKVVIRKKMTLP
                     ELYHSLSKLEHVCAIALEH"
BASE COUNT         3516 a         3360 c         3654 g         3692 t
ORIGIN      
        1 atttgtaaaa tcttaaaaag agcaggagat cgattatgtc caataaagaa ctcattacag
       61 aatttctgct ggcggcagaa caaggtaatt ccgatggatt aaaatcatgc cttgataaaa
      121 aggttgatat taatgcgact aaccgcgagg ggcgtactgc aattattatt gccagtctga
      181 ataaacatta tgactgcgtt tctttattga tttccgcagg tgctgatatt aataaacaag
      241 atcaaacttg ttttaatccc ttcctgattg gctgcctgaa taatgattta acactgttgc
      301 gtacggtatt accggctaac cccgacctta attgcctaac ccgttttggc ggtgttggta
      361 ttacacctgc ctgcgaaaaa ggccatgtcg agattgtacg tgaattgctg atgcgtactg
      421 atatcaatgt caaccatact aattttgttg gctggacgcc gctcctagag gccatcgtgc
      481 tgaatgacgg cggtgaaaag cagcaggaga tcgtcaaact gttgcttgag catggcgcaa
      541 acccgcacat gacggataaa tacggtaaaa ccccgctgga gctggcgcga gaaaaaggct
      601 accacaaaat tgccgagttg ctgatttcgg ctggagcctg acaacaggag cacgattatc
      661 gctatattat cacggcaatc ttttcgggca ctaaccgcag actgcgcgtt tctcactttt
      721 cggggaatgg gccaggttga acaggttttt tcccatgcgg taaatctgtt aatacctgaa
      781 cagcagcgac tgctgacgtt actgagtgag gattgtgata acgcacctaa cagttgtcgt
      841 ttagcactga cacattatga agaccttttt caccccggtg aacaggtcaa ttttagcggg
      901 tctggaataa ctgttggaaa tgataaatgg atagaaataa ccggttgtca gatgtggcga
      961 ataccacggt ggacatctga ttcagcaagc cttgccaaag tcggctggca gcactgggaa
     1021 gagtttatcc gccaacaact aagaagcaac gacacattat ttctgtacca gggtgacaat
     1081 ccattttatc aggaaatttc tcgccaatta ctcaaccgcc gaaaaaattt gctgaaggcg
     1141 ttgtgtacag gagaaaacat cactgcggca gtcaaccagt taattggtct tggaataggg
     1201 ctgacgccat catcggatga ttatttagta ggtttaagca ctattttatt tattcaagga
     1261 catccactaa ggaagtatag atacgatttc ttagcagcat tacaacttgc aggaaataac
     1321 ataacgcttc tcagcaaaat aacgttagag gaagcattta atcagcgtta tcgggaaaga
     1381 gtcggacggc tactgaacca atttattact gagccacaac ccatttctac gcaatatgtt
     1441 actgacataa aaaatatagg ctcaagttct ggatgcgaca tgctgtatgg catggcggat
     1501 gcctgcgccc tgagccacag gtccggaggt aattatgtca atcaggatag ttgtaaaaaa
     1561 aaatacgtat tttgattctg tttcactaat gtctatctcc acgcgcgcaa ataagcttga
     1621 tggagttgag caggcatttg ttgctatggc gacagagatg aataaggggg tgctgaagaa
     1681 tctcggtctg ctgacccctg agctggaaga ggcgaaaagt ggcgatctga tgattgtcat
     1741 taaaggtacc agcgaggagg cgaacgagcg gacgctaata gcgattgatg agctgtttgc
     1801 gcataaagaa cagggaggcc agcacgaagc acgctatgcc acgattgcca gtgcgaagaa
     1861 gcatgctcca gagagtaatc tggcggtgat ctctgtcaat ggcctgtttg ctgctcgtga
     1921 agctcgtcag gcactacaaa ataacctcaa tgttatgctg ttttctgaca acgtctctct
     1981 tgaggacgag ctggaactca agcatctggc acatgaaaaa gggttgttga tgatggggcc
     2041 tgattgtgga accgccatca ttaacggcgc agcgttgtgt tttggcaatg cagtgcgtcg
     2101 cggcaacatc ggaatcattg gtgcctccgg tacgggtagc caggagctga gcgtacgcat
     2161 tcacgaattt ggcggcggga tttcccagct gattgggaca ggtggacgcg atatgagtga
     2221 aaaaattggc ggcctgatga tgcttgacgc gattagcatg ctggaggccg atccacaaac
     2281 tgagatcatt acgcttatat ccaaacctcc tgcaccagcg gtagcccgta aggtgctgga
     2341 acgtgcgcgc acctgtcaca agcctgtggt ggtgtgtttc ctcggacgtg agtccacccc
     2401 tgcggatgaa gaaggcctac agtttgcccg cggtactaaa gaggctgcct tgaaagcggt
     2461 actgttgact ggtgtgaaaa aagagagtct ggatttgcat ccgctcaact ggcagctgat
     2521 tgaagaggtt cgctcccgtc tgactccaca acaaaagtat atccgtggcc tgttctgtgg
     2581 tggcacgtta tgcgatgaag cgatgtttac tgccatggaa aaactctcgg atgtgtatag
     2641 caatattcac ccgaatccgg cgttccgcct tacagatctc aaccgtagcg tggcgcatac
     2701 cttcctcgat ttcggcgatg attattttac caacggcaaa ccacatccga tgatcgatcc
     2761 caccaatcgc attacccggc tcttgcagga agcacaggat cctgaagttg gcgtgatcgt
     2821 gatggacttc attcttggat ttggatcaca tgaagatccg gttggagtga tgctggatac
     2881 aattgttgaa gccaaagaaa ttgctgccgc agacggtcgc ccgcttgaga tcctcggcta
     2941 tgtgctggga accgaccttg atacaccgtc gctgaaaaaa cagtgtcaga tgttgaccga
     3001 tgccggggtt atttgggcaa gcagtagcac caacaccgga ttactggcac gtgaatttat
     3061 atttaaaggg gaggaaggct gatgagccag acactgttta atcagccgct gaacgttatt
     3121 aatgtcggta tcgaaatgtt cagcgatgac atgaaaaagc agaatgtacc ggtaacccaa
     3181 cttgactgga caccgccagg ccaaggaaac atacaagtgg taacggcact ggacgacatt
     3241 gccgcttcac cactggcaga caaaattgca gctgctaatc agctggcgct ggaacgcatt
     3301 atccagtcgc atcctgtgct aattggctat gatcaggcga tcaacgtcgt gccgggtatg
     3361 acccgtaaca ctatccttca cgccgggccg ccaatccact gggaaaaaat gtgcggcgca
     3421 atgaagggcg cagtaaccgg ggctctggta tttgaaggac tggcgaaaga tctggatgat
     3481 gccgccgaac tagctgcttc aggtgaaatc atcttctctc cgtgccacga acatgactgt
     3541 gtcggctcaa tggcgggtgt cacgtctgca tcgatgttta tgcacatcgt cgagaacaaa
     3601 acctatggaa accgcgcata taccaatatg agcgagcaga tggcaaagat cctgcgtatg
     3661 ggcgctaacg accagagcgt tatagagcgt cttaactgga tgcgagatgt actgggacct
     3721 atgttgcgcg atgctatgaa gctggcgggc gaaatcgatc tgcacttgat gttagcgcag
     3781 gcgctgcata tgggcgatga gtgccataac cgcaacaacg ccgggacggc gctgctgatt
     3841 cagacgctga cgccgtggat tattcagact ggctaccctg ttgagcagca gcgtgaggtg
     3901 ttcgagtttg tcctcagtag tgattacttc tcaggaccaa catggatggc aatgtgtaaa
     3961 gccgcaatgg acgctgcgca cggcattgaa tacagcaccg tggtgacaac gatggcacgc
     4021 aatggtgttg agttcggtct tcgtattagc ggtttgccgg gcaagtggtt taccggtccg
     4081 gcgcagcagg tgattgggcc gatgtttgcg ggctataagc tagaggattc cggacgtgat
     4141 atcggtgata gtgcaattac cgaaacctac ggcgttggcg gtttcgctat ggcgacggca
     4201 ccagctattg tgtcactggt gggcggaaca gttgaggaag ctattgattt ctcacgtaaa
     4261 atgagagaaa tcactctcgg agaaaacccc aacgtcacca ttcccctgct tggttttatg
     4321 ggcgtaccga ccgctatcga tattacgcgt gtaggcagta caggcattct gccggtaatt
     4381 aacacagcca ttgcgcacaa agacgcaggg atcggcatga tcggcgcagg aattgttcac
     4441 ccgccgttca cttgttttga aaaggcgatc ctcaactggc gtgaccacta ctgccaataa
     4501 caataaccgg ggctaacgca gttagcccca cagtagcgga aaacgttgtt taccttcagg
     4561 gctgggcgat ctccatgatc gcaaacgatg cttaacctgt taaggagatt ccatgaacag
     4621 cataaaacta gaatggaaac gtggtgactg ggcggcttac tttggactga tgaccaacaa
     4681 tctgactaac ctgctgacaa tgatgggtct gctaattttt gtcgtcggaa tccctactga
     4741 aattgtctat ggacgcattg cgcctgcctt tggtcttgca gtgctggcgg ccagtgtctg
     4801 ctactcctgg tttggtctac agatggtgaa gaaaaccggt cgtagcgatg taacggcgct
     4861 gccgtctgga ccgagcgcac cctcaatctt taccgtgaca ttcctggtgc tgatgccggt
     4921 ttatcagcaa accaaagatg ccaactttgc tattcagata ggattggtgt ggtgctttgt
     4981 cgaagcgttg atcctggtcg gtggctcctt cctgggtgag actatccgca agatgatccc
     5041 gcgtacagtg ctgctttctt gtctgtccgg actggggtta ttactgctgg caatgaaccc
     5101 gatgctgcaa gcgttcgaag cgccgacagt ttctttcatc gtcctgttat tgatcttcat
     5161 taactggttc ggtaagaagc cgatttttcc ccatattcct accggtttgc tgttgctgat
     5221 tgctggtacg tccctagcgt ggatatccgg tttacaaagc ccggaagaca ttaaagcgtc
     5281 gatgtcttcc ttcggcttca acccgccgga aattcatgta aggagcttcc tgcaaggttt
     5341 gccgcacgct ctgccatatc tggcttctgc agttcctttg ggcctggcta actacatttt
     5401 tgacctcgag aacattgaaa gtgcgcacgc ggctggtgat gaatacaaca cccgtaaggt
     5461 catgatggcg aacggctttg cctccatgct cggttgcatg ctgggtaatc cgttcccggt
     5521 aaccgtatat gttgggcatg caggctggaa agctatgggc gctagtatct gctataccct
     5581 cgcatcaggc atcactatgt ttctggtgcc attgtttggg ttgggcgcgt ttatgctcgc
     5641 cattattccc atgactgcta tagtgccaat tctggttttt atcggtgtgg taaccgccaa
     5701 tcaggtggtg cgagaaactc ctaaagtcga ggttcccgtt attttcatct gtctgttccc
     5761 atgggtcgcc aactgggcat tgaccatcgt taacagcgtg atgggcgcag ccggaacatc
     5821 cgctgagaaa cttggtagcg acctgttgca cagcaaaggc gtctattacg atggactgat
     5881 gcatctggga agcggtgcac cgctggccag catgctgtgg ggatgcattg cgatctttgc
     5941 cattatgaat aagccgctgc gcggggcgat agctgcatcc tttggagcac tgttatcgct
     6001 gtttggcgtg attcactccc cagcggttgg ctttgcggaa ggcagctcga ttgtgtttgt
     6061 tgtggcctac ctgatgatgc ttgctatgtt tgccctcaag catgttattg atagccgtga
     6121 aacagtggct gcgtctgagc aggaagagac taaaacgacc tgaatagcct gcaaacggaa
     6181 gataattcta tgaaagaact tgtggtcgtt gccattggtg gcaacagcat tatcaaagat
     6241 aacgccagcc agtcgattga gcatcaggcg gaggcggtga aggccgtcgc cgatacggtt
     6301 ctggaaatgc tggcttccga ttacgacatt gtgctgaccc acggcaacgg gccgcaggtc
     6361 ggtctggatt tacgccgcgc ggagatagcc cacgagcgcg aagggctacc cttaacgccg
     6421 ctggcgaact gtgtggcgga tacgcaaggc ggcattggct atctgatcca gcaggcgctg
     6481 aacaaccggc tggcgcctca cggcgagaaa aaagccgtca ccgtggtgac tcaggtagaa
     6541 gtggataaaa acgatccggg gtttgtccat cccaccaagc ccatcggtgc attctttagt
     6601 gaaaaccagc gtgatgaact acaaaaggca aaccctgact ggcgttttgt tgaagatgcc
     6661 ggacggggct atcgccgcgt ggtcgcctcg ccggaaccga aacgtattgt cgaagcacct
     6721 gccattaaga ctctgatcca gcaaggcttt gtggtgattg gcgcgggcgg cggtggcatt
     6781 ccggtggtgc gtactgacgc aggggattac caaagcgtgg acgcggttat cgacaaagat
     6841 ctctctagcg cgctactggc tcgcgaaatt cacgccgaca ttctggtgat caccaccggc
     6901 gtggaaaaag tgagtattca ttttgggaaa cccaaccagc atgcactgga tacagtggat
     6961 gtcctaacga tggcccgtta catgcaggag ggtcacttcc cgcctgggag tatgttaccg
     7021 aagattcttg ccagtctgga gttcctggaa cgtggaggta aaagagtgat tatcaccacg
     7081 cctgaatgcc tgtccgcggc actgcgtggc gaaacaggca cccatattat ccactcccaa
     7141 gaggagacgt aaattaagga aagcgaccgc cgccgtgaat ttttaatgtt cattgcagat
     7201 tatcgtgggc tggaaatgat aacggttggg gagttattgt ttagttgcta aaacagaacg
     7261 ataaccaacc ccaaccgttc tcaatttcga atactatctt ttccatttat ctctggatag
     7321 gctttgacgt cgtccctact gagtaaaatc cccctcatca tccacctaaa accgattgaa
     7381 aacggtcatt attcttgtgg caaacaggct aaagccgttc acgatgcttc acttcgcacc
     7441 ttatcgtaat gaacaatact ggtatttcca gttcagttac gcctacccgt ctaccgcatt
     7501 ctttgcccta actacggcat agttcgggat tatatcagct ggctaaagcc tcatgctcat
     7561 cagaccacat gactcattga gcatgttgag gcgttactca aaatcttgcc gattaaacat
     7621 atttcgtaat ttcttaccct gcactaacat atcatcaata ctatcgacaa acgtcagttg
     7681 caaatagagg tcacagggtc tgattgatgt actgtcaagg ccgggtcatg aaagagtttg
     7741 ccctgttcaa agctcatcgt tacgcaccgt cgtagccgat gcggatactc atcaatcctc
     7801 tggataggtg aggtgcgtag tagagaagct cggtcgtttt tcgttaatct gaatcagtac
     7861 tgtcaacata ttcaagtcgt taatatggat atgaatacct cctttgacct agaggtacag
     7921 atgcactacc ccccaagctg tcaacgacgg atgaaaagtg gctcttccgc aacctggatg
     7981 gagattttca tgccagtgga ctcatgaccc tctgcctgag tagttcaaat ccggctcgac
     8041 catacatctg gcctttcagc atttttagcc gatttacatg actttcgaca ccccccatta
     8101 ttccagcgac tgctgattgc ctcacatata gccgcagcat cagctgccat ccttgcagcc
     8161 acgcgccgaa gttaccactt tctcgttgct cttttaacca ctccctccat gggtccagaa
     8221 gtcctcgctc tggaggccgg gttgacattt cagggaatgt tcctgaacac acccagagac
     8281 tgactgtcac acgtgataag cctgtaatac gggatatttc ccctgaaccc acatcccttg
     8341 ttatgtagtg ccataacctc tgtccaacgc tgatgccgtt tttggcgggt ttgctgttta
     8401 aggcgttccg gacgacggag cgatgctaca ggcacagaca gtactggctt aggtgatttc
     8461 tttggtgaca acccactggc aacaagacgt atcagaggca tctgtctgta catcattcgt
     8521 tcaggcgcat cgccaatatt ttttagcagg tgccatcgat cagctatctg tctggcctga
     8581 ggtgcacctt cacgtgctgc tgtcgcatag actccgccac ggtcacatga gacaacctgt
     8641 atttccggat attttctgaa ccaggtcgcc agcgtactct gatcgcaacc gggaagcagg
     8701 acgaggggac ggtgagtatc aagattgacg attaacgtac cgtaatggta gtcccgatgc
     8761 cacgcccact catcaatacc gacatgaggc gcgcctgact gtttcgtctc cggggtatta
     8821 ataaccctgc gaagaagagt atcagcactg caacggagcc ccactgccgt tgctgcctgt
     8881 ttcccggcct cacctccggc tattaatccc agttgatgtt gtaaattttg taacacctgt
     8941 gaagaatgct gggagtgaac cggcgaaggg ggcaagcgac ccggcaaaaa ttttacgtga
     9001 acaagccggg ttacggcagt accagtggcg gacggaaaat acaagccata gcgtctgccc
     9061 tgagcagggt agatgctgta tccggcgccg acgataacta tgaacagagt cgctacgctt
     9121 aaagcattca ggacttgtgc tgttttgcgt tttccgtgga gatggagggt tataccgtca
     9181 gagctaataa tttgtcgact gcatctccat ccgcaaggta actgtagaag tgtcttaaga
     9241 gagtgcattg cgttgcccat aacttaaaac agcttagatc tttattcacc agaaagtttc
     9301 atccattttc ttccacccag gttgcggaag agccactttt catccgtcgt tgacacctta
     9361 cggtaataat aaaccatatc cagcctgtac tctgcagata aacagctctg tgtagcgatt
     9421 cagtttcatg atgaaaatac agtagggtaa ttataaacac aggtatatca ggatctgtaa
     9481 cctttcaagg atggtgatgg tcgggagttt gagtgtgact catgcttgtc gctactgaat
     9541 acgctggtgt attgctgatg ttttaagcgt aaattagccc ggcaggagcg gggatacaga
     9601 caacgccagc tttgcgctta caccttatgg gtgatctcta tcacgatttt acttccattg
     9661 actggtttgt ctacgcgagt agatttattt atatgcaata aaacacaata tctacatgag
     9721 tagatttaag aaagatatct acctgtgatg tatatctgac agataatatc aaggagttct
     9781 tatgaacaca catcatccat catcctctgt tcccttagtg gataaccaac aggcagctca
     9841 ggagcgtctg gaaacccccg agggacgtcg cgagttttgg agagctacct tttcctgctg
     9901 gctaggcact acaatggagt atgccgattt tgctctgtac ggacttgccg caggtattat
     9961 ctttggcgat gtcttttttc cggaggtgac acccgtcata gcattacttt ccagctttgc
    10021 cgcctattct gttgggttta ttgcccgccc tattggtgca ttactgttcg gctggatagg
    10081 cgataaacat ggtcggaaaa ttgtcatggt tatcactatt ggattgatgg gcatgtcgac
    10141 catgctaata ggattaatcc ccagttacgc ccagataggc gtctgggcac cgatatgtct
    10201 ggttatcctt cgattttctc aggggctggg agctggcgcg gagctttcag gcggtactgt
    10261 gatgcttggt gaatatgctc ccgttaaacg acgtggacta gtttcatctg ttattggtct
    10321 ggggtcgaac agtggaacat tactggcttc actggtttgg ctcatcgtcc tgcaaatgga
    10381 caaggatgac ttattaaact ggggatggcg tattcctttt ctttgtagca ttcttattgc
    10441 tgctgccgct ctattaattc gtcgtcatat acgcgaaaca ccagtctttg aacgtcaaaa
    10501 agcccttctg caggctgaac gagaaaaggt tcttcgtgag ggaaaagcac aacaacaaca
    10561 tgacactcgt agcttctgga aacggacccg tgccttctgg attatggtcg gattacgcat
    10621 aggtgagaat ggtccttctt atctcgctca ggggtttatc attggttatg tcgctaaagt
    10681 actaatggta gatgagtccg tacccacggc agccgtactt attgcatccg ttctgggatt
    10741 tgccattatt cctctggcgg gttggctgtc cgatagattc ggtagacgta tcatctatcg
    10801 ttggttctgc ctgttactga tcctgtatgc ctttccagca tttatgctgc tggattcccg
    10861 tgagccgtgg attgttatcc cgacgatcat taccgggatg gggcttgctt cactgggtat
    10921 ttttggtgtt caggctgcgt ggggcgttga gcttttcggt gtcactaatc gttataccaa
    10981 aatggcattt gcaaaagagc tcggttccat tatgtctggc gggacagcgc cacttatcgc
    11041 ctctgcgcta ctctcgtttt acggacactg gtggccaatc gctatctatt tcgcctttat
    11101 ggccgcgatt ggactggtga ccacgttctt tgcaccagag actcgcgggc gggatctcaa
    11161 cttacccgag gatgcaattt aaggccagag aacgtgagat gcgcgttctt taccaaagat
    11221 ttttgggtca taacgcctta tgctgtctgg aaaaaatcac tctgaacgca attaggtagg
    11281 tgcttcgtga ctcaatcaca ttcacaacgc gtaacacgct ctgacgtggc aaaagaagca
    11341 gggacatccg tcgctgtcgt tagttacgtt attaataatg gtccgcgccc cgttgccgaa
    11401 gcgactcggc aacgtgtact acaggcaatt aagaaaaccg gttatcgacc caatggcatc
    11461 gcgcgtgcgc tggcttcagg gagtacgcaa acttacgggc tagtcgtgcc ggacatctcg
    11521 aacccgttta tcgcttctat agctcatgct ctgcagcacg aagcctttgc tgatggcaaa
    11581 gtactcctgc tgggcgacgc aggcgacagt agctgccgtg aacgtgaact cattaataat
    11641 atgctgcacc gtcaggttga cgggctgatc tacaccagtg ttgatcgcca tccttatatc
    11701 aatttgattc aggagagcgg taccccctgc gtcatgctcg atcgtgtgga tcctggactt
    11761 aacgtcagcg ttatccaggt tgatgaacaa ctggcggcaa tgctggtaac acaacatctg
    11821 attgatcacg gataccgcga catcggcatc atctgcggcc cacgtgaaat gctgaatact
    11881 caggatcgta tccggggttg gcggcaggcg ctggaagcct catcgttagt ggtcaatccc
    11941 tcatggattt tttcgaccaa ctatacccgc gccggcggtt acgaagcgac aaaacgcatg
    12001 cttgagaacc aactgccacg cgccctgttt gcgacgaatg aacaacaggc tctcggttgt
    12061 ttacgcgccc tggcagaaca tgggctgcgc gttcctgaag atgttgcgct ggtctgcttt
    12121 aatgcaacac aagaatcggt ttacaacgtc ccttctttaa ctgctgttcg acaacctgtc
    12181 gataagatgg cccgtgcggc aattgacatg ctgaaaaatt gggatggaga agttcgccgc
    12241 gttgaatttg agttttattt acgaacagga gagtcgtgtg gctgccaagg gcatgaagta
    12301 caaccagaga aaaagtgaaa atgaatcgat gcgtatcatt attgactgcg atccggggaa
    12361 cggcattccc ggcgctaata tcgacgatgg tctggcgctg gcgctggcca ttgccgcacc
    12421 gcaaatcgcg ctggaaatga tcacaactgt tgccggaaat acgccggtag acgtgggata
    12481 tgcggtcgct agagatctca ttacacaatt ggatattcct gttgcggtat accgtggtgc
    12541 ctcgcgtgcg ctccgggaag atccgcaacc ctggcgtgaa aaactggatc atggtgtcga
    12601 tcagtttggt ttacggcaac tctggacgaa tgttcctgcc ccagcattgt accagaaggt
    12661 agaacctcat gcgcctgaag caataggtga actcatctgt cgtaatcctg gcgaaatcac
    12721 gttggttgct accggccctc tcactaatgt agccatcgcc ctgcagcttt acccgcagat
    12781 tgttcacgcg gtcaaaaata tcgtagttat gggtggcgtg ttcaatgttc ctggctacct
    12841 gaaagataca aattttggtc tggaccctga ggctgctcat gcagtgctca ccagcggtgc
    12901 gccagtcact ctggtcccga tggatgtgac aacgcaaaca caaatgcttc acgccgattt
    12961 ggaacgtctg gcaaaaacag aaaacgggct tagccgttat ttggcacaaa ccattcgacc
    13021 gtggattaca tactctatgc aaacccgcaa tctgcctggg tgttggatcc acgatgtgtt
    13081 aaccgttgct tggttactgg atccctctct tgcaacaacg gctgaagatt atctggatgt
    13141 ctcactggaa ggcattacac gcggaatgac ttgttgctat ggacgtgaca cattacgcct
    13201 caatgttggg attcccgaac caaaaggtgc acgggtcaca attctgcaga gcatcgataa
    13261 cccgcggctt atttcgctga tagagcacta tatccagagc tacggcgcgt agcgtcatgg
    13321 ggcagaaaag gagaccttcg tacacagttc ggggtgattt ttttccggag gaaatatgaa
    13381 tacgttgtcc ttcctcttct tcggtgaagg agaatctctc ttaattacac tgggcaaaat
    13441 ttgcgtcgca tttatcctgg gtggcattat tggccttgaa agagaatcga aagggaagcc
    13501 tgttggattt aaaacgtgcg tcattatttc agtcgccagt tgtgttctga caattgtatc
    13561 gattcagtct gcagaatatt acgctgaaat ctcgatgaat atacgtagcg atccgatgcg
    13621 actggcagcc cagattatca gcggtgtagg ttttctcggc gcaggtgtta tccttcatcg
    13681 ccatgacgat gctatatctg gactaacaac tgcagctatc gtttgggctt cggctggggt
    13741 gggcattaca tgtggtgctg ggttttatat gcatgctttg gttaccacgg ctttgttcat
    13801 gattgctata aaactcagct attttgtgct ctttttgcaa gctaagaatc aatttccggg
    13861 taaagtaaaa gttcatgtta ctcttgacga aagatcaggg ctacaagccc tgatagagtg
    13921 tattaatcaa caaaaaaata ttattgaagc attcactatt cgcgatgtta aaaagggaag
    13981 aatagaagtt aaccttaaag tcgtgattag gaaaaaaatg acattgccag aactatacca
    14041 ttcactctct aagcttgaac atgtttgcgc tatcgcgctg gaacactgaa agcatcgatt
    14101 agaagctaaa acagcaacaa gaacaatcac aataacaaat tggtcaagtc tggatatcat
    14161 atgttcacag aggcaggatg ctctgtgtta attagtactg cagaggaggc ccggcagtgg
    14221 aa
//
LOCUS       DBBGLQ010000113        14216 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.113, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000113 DBBGLQ010000000
VERSION     DBBGLQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.113"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..262
                     /locus_tag="ACIYSB_004350"
     CDS             <1..262
                     /locus_tag="ACIYSB_004350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431264.1"
                     /translation="AKQLFSMLIFKTAINKVKQKTPIKNGAENSAPSRVTSPEVISLF
                     SGDHSRRGFHGVRAPCNTTGRSGRCCIRNRATPLFNRHSVSR"
     gene            complement(322..2340)
                     /locus_tag="ACIYSB_004351"
     CDS             complement(322..2340)
                     /locus_tag="ACIYSB_004351"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGC5431265.1"
                     /translation="MRLFLCEKPSQAGDIARVLGAARRVDGAIEGKDFVVTWAFGHLL
                     EQAPPEAYGEQFGSPWKIDVLPVFPEQWQMVVKDNVGKQFNVISRLLRKADEVVIATD
                     ADREGEIIARELLDYCGYTGVVRRLWLSALDESSIREALGRILPGEETEKLYEAGKGR
                     SRADWLTGINLTRLYTLKARECGVSGVLSVGRVQTPTLAMVVRRDAEIKNFIPVPYWQ
                     LYLWLEKDNVRFRTSWIAAAQYCDTEKRCVNVQAATAVLSQCRQAGVAVVRDVSSRRE
                     KKQPPLCFSLGTLQEVCSRKFGLGAQAVLDIAQSLYEKHKATTYPRTDCGYLPVSMYK
                     EVPDVLAALEKTDPDIAPELAKLSPDIVSGIWNDRKITAHHAIIPTRQPFNIGALSDN
                     ELKVYRLIRQHYLAQFLPVQELDVTEATFLVAGQLFGTRGRVEVVRGWRALFVSDSAG
                     EEEDVADDEQAMPLPVLAKGDECRPAEAELKEMQTTPPRHYTEGTLIAAMKNAAGMVS
                     DPALKKVLRENAGLGTEATRAEILETLFTRKYIEKQGKHIRATQLGAELIAGLPEALT
                     SPGTTALWEQALDDIAQGKLSLSSFMEKQKQWLNHLVAGARQQQLQITAPVTPACPLC
                     GSRMRKVKMKSGKGEFWSCQRYPECKGVISEQKPTSGKRGGRKIKKHG"
     gene            complement(2357..3082)
                     /locus_tag="ACIYSB_004352"
     CDS             complement(2357..3082)
                     /locus_tag="ACIYSB_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HGC5431266.1"
                     /translation="MSEEQMKEPASRRVGALTSPVNIELHSRYAIMLWEGRRGTKSKS
                     AEDRRAAKIMSMPEAIKMTGGLYRDSLADNPYADETMWKVENAIVEATDKINAEVRVL
                     EGILRNIPSVITVRDALSRSPVDIGVFSRSPLGYRCVWLLVGYDQLALKTFQAAHLGL
                     ISRNRRDELLSSGAHLIRKIYGILQGYRTMAVTRDDIENKTEKAEKAIARYGMPHDDI
                     LSGKLRSSFSPPLNRKSRDASRQ"
     gene            complement(3184..3762)
                     /locus_tag="ACIYSB_004353"
     CDS             complement(3184..3762)
                     /locus_tag="ACIYSB_004353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431267.1"
                     /translation="MVKNRTVLAVPTVVKNCAVLAVSTVVKNCAVLAVPTVIKKCTVL
                     AVPTVVKNCAVLAVPTAVKKCTALAVPTVIEKCTVLAVPTVIKKCTVLAVPTVIKKCT
                     VLAVPTVVKNCAVLAVPTVVKNCAVLAVPTVIKKCTVLAVPTAVKKCTALAVATAIKK
                     CTVLPVATVVKKCPALPVATVFENCTELALPT"
     gene            complement(3762..5027)
                     /locus_tag="ACIYSB_004354"
     CDS             complement(3762..5027)
                     /locus_tag="ACIYSB_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HGC5431268.1"
                     /translation="MALPAESLIAHTLRKMKASMAEKASREDGRIRSGLLFTGNVHDS
                     YPRRLLLDTRLSPLDKTAWMMIRLHALNNEGAVFPTYDELQLQLASPGCGKASRETVS
                     RVLTMLRLTGWLSLCKRVRDEKGRVRGNIYALHDEPLTCCDAEKFDPRYLDMVLDTVS
                     RSRNKTIRHTASEVLSDIKNEPGMRHYHSHITLFESRMGAAQTPGQLAARQRVNPMVN
                     ENTGRESGSDSVHTPAPVVQVTELSAPLTELSEKQEMSPLSSDSVPPEKSVTYHSVRK
                     PNNYVRNITHSVIKNTYVLPESVKSQISTEDTDMLTKQLQALPEELAGSVLSSLSQML
                     SKNTLQNPVGWMLAVMKRAREGSFNTPDMSVHESRSPARAPYQPVYNRDERERRRRKV
                     ASPETVSNIVAEFRASLKRANAARLAARV"
     gene            complement(5288..5509)
                     /locus_tag="ACIYSB_004355"
     CDS             complement(5288..5509)
                     /locus_tag="ACIYSB_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431269.1"
                     /translation="MKLKMQAMDGPVIIESSDVTQFYPDHESGGELTAVEYLADGGRI
                     TARVRHSFYQVAAALAGAWRADDASKSGG"
     gene            complement(5506..6012)
                     /locus_tag="ACIYSB_004356"
     CDS             complement(5506..6012)
                     /locus_tag="ACIYSB_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431270.1"
                     /translation="MTTITRENAEIKSFITGFLSDAAHDKQSSNSLLANVFRIALASL
                     EAEPVAWRYRYVKKGVTDSQGESWVGDWKYVPTKEDCNDRPNYEIQALFTAQPVPLTQ
                     EVLNLEKARMQLRVLLSGPVPAITPMSHAVAIKVVPDAPDASERRVAELEQACAAAGI
                     RIKGEQVK"
     gene            complement(6009..6218)
                     /locus_tag="ACIYSB_004357"
     CDS             complement(6009..6218)
                     /locus_tag="ACIYSB_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431271.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            complement(6472..6606)
                     /locus_tag="ACIYSB_004358"
                     /pseudo
     CDS             complement(6472..6606)
                     /locus_tag="ACIYSB_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6603..6968)
                     /locus_tag="ACIYSB_004359"
     CDS             complement(6603..6968)
                     /locus_tag="ACIYSB_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGC5431272.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFAGN
                     MLHQDRDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            complement(7082..7195)
                     /locus_tag="ACIYSB_004360"
                     /pseudo
     CDS             complement(7082..7195)
                     /locus_tag="ACIYSB_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(7182..7439)
                     /locus_tag="ACIYSB_004361"
     CDS             complement(7182..7439)
                     /locus_tag="ACIYSB_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431273.1"
                     /translation="MSNAARLQLGFSPFSKTIVLAKMRNLDDGTSLRVGNDPGRDVTN
                     EAAQLVWHLVMAEGGEIMWEMDDGSRMVLKAESQEATSEQD"
     gene            complement(7449..7697)
                     /locus_tag="ACIYSB_004362"
     CDS             complement(7449..7697)
                     /locus_tag="ACIYSB_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431274.1"
                     /translation="MKMKELVPGLLSCPGVPPACQRKAVDLVNRFRRGERNYHELKEK
                     GFGYIKINVGPDWRLLSRNKGLTWALLSHERYNNMRLK"
     gene            complement(7694..8272)
                     /locus_tag="ACIYSB_004363"
     CDS             complement(7694..8272)
                     /locus_tag="ACIYSB_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HGC5431275.1"
                     /translation="MSNNLSQATNGLLMQLVMDLKSGYLRRCEALGLSREEMQMLQGL
                     SIEEIHYLSNSEVSVLRLDINHNNLVRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRISGIDVRPGRGITLSDDENSELWRLWQKAGINDVESADGLDVMML
                     CAEQMNIPLTAVWHAVRGWHTAGSPEQVRNAS"
     gene            complement(8269..9000)
                     /locus_tag="ACIYSB_004364"
     CDS             complement(8269..9000)
                     /locus_tag="ACIYSB_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HGC5431276.1"
                     /translation="MNNDAARQEQLIKRIRKLLALSRNNSNPHEAAQALSRAQALMRK
                     HGITELDADISVIKTSSSKGAPSEAEKVPVWMGRLMNVVAGAFGCHTYLDWRITTGGY
                     WRRIVTFYGFGERPVVAAYAFDVLCRQMQKATKEYLSTQSRKLKHSTRRARAAMFRDG
                     WVYGVRDVVDAFRLTEDETRVMKTWLQNQQFEGMVVFREVKDCNGGDHARWQGYNEGK
                     KATLNHGVDSRDLRPESLISGGGEV"
     gene            complement(8993..10642)
                     /locus_tag="ACIYSB_004365"
     CDS             complement(8993..10642)
                     /locus_tag="ACIYSB_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HGC5431277.1"
                     /translation="MSIKRNHSVDLGAAIMQPGRQASTAGNVVALGETPMILTLDQLR
                     PNPDNPRTTRNPRYDDIKSSIFSRGLDTVPKVTRIPESEPDIYIFSDGGNTRYQILKE
                     LWEETGDQRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKARGIHKARMIYEE
                     QLSKSVSMRELSSLLTEQGLPVDASSISRMENTLKYLYPWIPELLESGMGSPQVRALL
                     SLRQDAERIWGQFAVATENGGDFDRVFGECCSKFNSPELWSLEMFRDELIGDLLKALP
                     HPVLDYDRWLLELDPRERNRRHHFGEPEPVLIPADSDAMPEPVTHGTSAVTVEKDSGR
                     EQSVISAPADHDTVMNTEQSPVGKAESSVTEQREERRRKEVQPDMYGGEETYSGDYEE
                     DGDTRDAADLPVTSPEEGAQQSSGEIFSEPEFSVGELDDIEYLQNEAFHLAWELASHT
                     GCVDELAMDRESELSAGFGAAEANCSPVMAFLVRLTGEEVLRVNPVSLDALLIGGSTE
                     EEPPLLDDEHAGKLLRLLVVLRRLRGLQRQVEEENVEGRDE"
     gene            complement(10632..12020)
                     /gene="dnaB-PI"
                     /locus_tag="ACIYSB_004366"
     CDS             complement(10632..12020)
                     /gene="dnaB-PI"
                     /locus_tag="ACIYSB_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HGC5431278.1"
                     /translation="MSERYIPRSVDAEQAVLGGLMLDNERWDEVVLMLRGEDFSLAAH
                     RVIFRTMSELATSGQPFDLITLSEHIEKTNNIEALGGFAYLAELSKNTPSAANIIAYA
                     RIVAEKSMLRQLQETGNALITDVAAPDATPRTVLESAERRLFSLSQTGMLHERCEVSL
                     TSAMELALRQLEEGLNAGGMTGTPTGFDELDEMTCGLQPGDLILLAARPSMGKTALGL
                     TFCLNALCNRTDETVFLFSIEMSREQLILRVLSMLSRVELTRLRSSMMDDEEWARVTA
                     TMGRFMNEASFIGDGNRLIIDDTSFQTPSSLRASARRYVRLYGRPSLIMVDYLQLIRS
                     PDQENRTQEIAEISRALKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNSGTLDKGVAEIIVSKQRQGPTGTIRVNFDGRYTLFSPFERGW
                     NFDRAGGNAYVN"
     gene            complement(12013..12525)
                     /locus_tag="ACIYSB_004367"
     CDS             complement(12013..12525)
                     /locus_tag="ACIYSB_004367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431279.1"
                     /translation="MLMNKSKLNGLLNADTVIVQKKPAAAVNQPVSEPAKSVSELPVS
                     SGKDFCSLVKEGFAPFHIVGAHTSDFPVFIIFCHVAILIMVVINAGRQLLMICVHKVF
                     LFVNMLIMSANMLYVPSDRGRQTYRSENVQKVMGLLCPATVRPAPVFVFLHGGFAVKQ
                     APFAREVAYV"
     gene            complement(12512..13006)
                     /locus_tag="ACIYSB_004368"
     CDS             complement(12512..13006)
                     /locus_tag="ACIYSB_004368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431280.1"
                     /translation="MNNSSNTTTRNTLSPVESACHALLDFIAREVFENKAPEEDVRER
                     CKVFTALLNEGFKALLDEAGLNLSNLRKQCEELESQITRLDSQSNAICRRGAQIERFI
                     GHHLPFFDLNKVFDTASQSCESAPSPAQSAVSSSQPSNLSEQGTSQRVSSVQPLQGAS
                     DVDE"
     gene            complement(13095..13973)
                     /locus_tag="ACIYSB_004369"
     CDS             complement(13095..13973)
                     /locus_tag="ACIYSB_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HGC5431281.1"
                     /translation="MKILPVVSTKGGEGKSTQSANLAGFLADAGIRTLLIDGDHAQPT
                     ASNIFPLTYEASAGLFELLMRTADLSYPDNIISRTAIDGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFLNNYDVIIVDSQGARSVMLEMIVLSATDSVVGMVNPVLPDV
                     REFIRGTVNVMENLLPYRELGIPLPKVRTLVNCMDYTALARKTLDELAGIIQTGRYSR
                     QLPEGAVSLLSTQIYDLNIYKQGHAAGQPVHRLEKESTRRSDSALVTMHSLACELFPE
                     WKQAFDGLLANGGIRQ"
     gene            14039..14116
                     /locus_tag="ACIYSB_004370"
     tRNA            14039..14116
                     /locus_tag="ACIYSB_004370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14096..14098,aa:Ser,seq:cga)
BASE COUNT         3335 a         4171 c         3198 g         3512 t
ORIGIN      
        1 tgcaaagcag ctatttagca tgctcatttt taaaacagcc ataaataaag tcaagcagaa
       61 aacaccaata aaaaacggcg ctgaaaattc agcgccgtca cgggttacca gccctgaggt
      121 tatctcactt ttctccgggg atcactcccg acgcggtttt cacggagttc gtgcgccttg
      181 caacacaacc ggacgcagtg gtcgttgctg tatcagaaac agagcaacgc cgttgtttaa
      241 tcgccactct gtttcccgct gataacgggg gtatacatca aagcgtatgt actttaacaa
      301 ataaaaaaaa gcatgtaatc atcatccatg ctttttaatt tttctgccgc cccgtttccc
      361 ggatgtcggt ttctgctctg aaatcactcc cttacactcc ggataacgct ggcagctcca
      421 gaattcccct ttcccgcttt tcatcttcac tttccgcatt ctgctcccac aaagagggca
      481 tgccggcgta accggtgccg tgatctgcag ttgctgctgc cttgcaccgg ccaccaggtg
      541 gttaagccac tgtttctgct tttccataaa tgacgacagt gacagtttac cctgtgcaat
      601 atcatccaga gcctgttccc acagcgccgt tgtccccgga ctggtcagcg cctccggcaa
      661 tcccgctatc agctccgccc ccagttgcgt ggcacggata tgcttaccct gcttctcgat
      721 atatttgcgg gtgaacaacg tttccagtat ttctgcgcgg gtcgcttcag tgcccagccc
      781 cgcattttcg cgtaacactt ttttcagtgc cggatcactc accatccccg cagcgttttt
      841 catcgccgca atcagcgtgc cttccgtata atgacgcggt ggcgtggtct gcatctcttt
      901 gagttcagct tcagcaggac gacattcatc gccttttgcc agtacgggaa ggggcatcgc
      961 ctgctcatcg tctgcaacat cctcttcctc tcccgccgaa tctgacacaa aaagcgcacg
     1021 ccatcccctg acaacctcca ctcgcccccg cgttccaaac aactgccccg caacaagaaa
     1081 agtggcctct gtgacatcca gctcctgaac gggtaaaaac tgagccagat aatgctgacg
     1141 aattaaccgg tacaccttaa gctcattatc actcagcgcc ccaatattaa acggctgacg
     1201 ggtggggatt atcgcatgat gcgcggtgat cttcctgtcg ttccagatcc ccgacacaat
     1261 atcaggactg agttttgcca gctccggggc aatatccgga tcggtttttt ccagcgctgc
     1321 cagtacgtca ggcacttctt tgtacatcga cacaggcaga taaccgcaat ctgtacgcgg
     1381 atatgttgtc gccttatgct tttcgtacag cgactgggct atatcaagaa cggcctgcgc
     1441 gccgaggcca aatttgcgcg aacagacctc ctgcagcgtt ccgagcgaaa aacacagcgg
     1501 aggctgcttt ttctcccgtc tgctgctgac atccctgaca acagcaacac cggcctgacg
     1561 acactgtgac aggacggctg ttgccgcctg cacattcaca caccgttttt cagtatcgca
     1621 gtactgcgct gccgcaatcc aggaggtacg aaaacgcaca ttgtccttct ccagccagag
     1681 atacagttgc cagtaaggta ccggaataaa atttttgatt tctgcatccc gtctgaccac
     1741 catcgcaagc gtgggcgtct gcaccctgcc aacagagagg acgccggaca caccacactc
     1801 cctggctttc agggtataca gacgcgtcag gttgatcccc gtcagccagt cagcccggct
     1861 gcgtccctta ccggcttcat ataacttctc ggtctcctct ccgggcagta tcctgcccag
     1921 cgcctccctg atactgctct catccagtgc ggacagccag agacggcgca caacgccggt
     1981 ataaccacaa taatccagca gttcccttgc gataatttcg ccttcacgat cggcgtcagt
     2041 ggcaatcacc acttcatcag ccttacggag aaggcggctg atcacattaa actgcttccc
     2101 gacattatcc ttcaccacca tctgccactg ttcaggaaag accggcaaaa cgtcaatttt
     2161 ccagggactg ccaaactgct caccatacgc ctccggtggc gcctgctcca gcaagtgacc
     2221 aaaggcccag gtcacaacaa agtccttacc ttcaatcgca ccatcaacac gtctggcagc
     2281 cccaagtact ctggctatat caccggcctg tgaaggtttc tcacacaaaa agagacgcat
     2341 gaattctcct cacctgtcat tgccgggatg catcacggga cttacggttc aggggcggag
     2401 aaaatgaaga tctgagctta ccggatagaa tatcatcatg aggcatacca taacgggcaa
     2461 tagctttctc tgccttttct gttttattct caatatcatc ccgagttacc gccatagtgc
     2521 gatacccctg cagaataccg taaattttgc ggataagatg cgcaccagaa gacaacaact
     2581 catcacgcct gtttcgtgaa ataagcccca gatgtgctgc ctgaaacgtt tttaatgcaa
     2641 gctgatcata cccgacaaga agccatacac agcggtagcc cagaggagag cggctgaaaa
     2701 ccccaatatc gacaggagaa cgagaaagag catcccggac agtgatcact gacggaatat
     2761 ttctgaggat cccttcaagt acacgaactt cagcattaat tttatcggtt gcctcaacaa
     2821 tcgcattttc aactttccac atcgtttcat ccgcataggg attatcagca agagaatctc
     2881 tgtagaggcc gccagtcatc ttaatagctt ccggcatact cattattttc gctgccctgc
     2941 gatcttcagc acttttactt ttcgtgcctc gtctcccttc ccacagcatg attgcataac
     3001 gggagtgcaa ctcgatattc actggtgaag taagcgcacc tacacgacgg gatgcgggtt
     3061 ccttcatttg ttcttcagac atgttatatc tccggcaaaa gaaaaggata tcgggctgaa
     3121 atagtcagcc tgcagacgga gatctgacaa tcaaaaataa aaaaaggaat gactgaaatt
     3181 acgtcatgtt ggcagtgcca actcagtaca attttcaaac actgttgcca ctggcaacgc
     3241 cggacacttt ttaaccactg ttgccactgg caacaccgta cattttttaa tcgctgttgc
     3301 tactgccaac gccgtacact ttttaaccgc tgttggcact gccaacaccg tacatttttt
     3361 aatcactgtt ggcactgcca acaccgcaca atttttaacc actgttggca ctgccaacac
     3421 cgcgcaattt ttaaccactg ttggcactgc caacaccgta cattttttaa tcaccgttgg
     3481 cactgccaac accgtacatt ttttaatcac tgttggcact gccaacaccg tacatttttc
     3541 aatcactgtt ggcactgcca acgccgtaca ctttttaacc gctgttggca ctgccaacac
     3601 cgcgcaattt ttaaccactg ttggcactgc caacaccgta cattttttaa tcactgttgg
     3661 cactgccaac accgcacaat ttttaaccac tgttgacact gccaacaccg cgcaattttt
     3721 aaccactgtt ggcactgcca acaccgtacg atttttaacc atcaaacacg cgccgccaga
     3781 cgggcagcat ttgcacgttt caggctggca cggaattctg cgacaatgtt gctcaccgtt
     3841 tcaggtgacg ccactttcct gcgacgacgt tcacgttcat cacggttgta cacaggctga
     3901 tatggtgcac gggctggcga ccgggattca tgcacggaca tatccggcgt attaaaactt
     3961 ccctcacgcg cacgtttcat cacagccagc atccagccaa ccggattctg gagcgtgttt
     4021 ttgctgagca tctgtgacag gctgctcagc acactacctg ccagttcttc cggcaacgcc
     4081 tgcagttgtt ttgtcagcat gtccgtgtct tctgtgctga tttgcgattt caccgactca
     4141 ggcaatacgt acgtattttt aatcacactg tgtgtgatat tacgtacgta attgttcggt
     4201 ttccgaaccg agtgataagt tactgatttt tcaggcggta cggaatccga actgagtggg
     4261 ctcatttcct gtttttcact gagttcggtt aacggagcac tgagttcggt tacctgcacg
     4321 acaggtgcag gggtatgtac ggaatccgaa ccactctctc tgcctgtgtt ttcattcacc
     4381 atcgggttca cccgctgacg cgccgccagc tgaccaggtg tctgtgctgc ccccatacgc
     4441 gattcaaaca gagtgatatg gctgtggtaa tgccgcatac cgggctcatt tttgatatca
     4501 ctgagcactt cactggctgt gtgccggatt gtcttgttcc ggctcctgct cacagtatcc
     4561 agaaccatat ccaggtatcg tgggtcaaac ttttctgcat cacagcaggt cagtggctca
     4621 tcatgcaggg cgtaaatatt cccccggacc cgcccttttt catccctgac ccgcttacac
     4681 agactcagcc agcccgtaag ccgcagcatt gtcagcaccc ggctcactgt ttcacgggac
     4741 gccttcccgc acccgggaga tgccagttgt aactgcagtt catcgtacgt cggaaatact
     4801 gcgccctcat tattcagggc atgcagacga atcatcatcc acgcagtttt atcaagggga
     4861 gaaaggcgcg tatccagcaa cagcctgcgg ggatacgaat cgtgaacatt gccggtaaac
     4921 aacagcccac tgcgtatcct cccgtcctca cgactggctt tctctgccat gctggccttc
     4981 atttttctga gcgtatgcgc aataaggctt tctgcaggta gcgccataaa taaactcctt
     5041 aaatcccggc agactgcgcc ggggaacggt ttccgaaatg cttataactg aaccctgtca
     5101 aagatgcagg ctaaacactt caccacacga gcaccaccgg actgactgtc gcgtccggct
     5161 tcagaagata ttttccttaa ctgttgatac gctgacgctc ttcgtttgcc ggctgcttca
     5221 ccgcaacata aaaaatttat gtcgcggttc agcagcttcg gaacagacaa tcattccggc
     5281 acagtcctca cccaccgctt tttgatgcat catccgcacg ccatgccccg gcaagtgccg
     5341 cagcaacctg ataaaaagag tgcctcaccc tcgcggtgat tcttcccccg tcagcaaggt
     5401 actcaacagc ggtgagttcc ccgccacttt catggtcagg gtaaaactgt gtcacatcac
     5461 tactctctat aatcaccggg ccgtccattg cctgcatctt cagtttcatt tcacttgttc
     5521 ccctttgata cgaatgcccg cggcagcgca ggcctgttcc agttcagcca ccctccgctc
     5581 actcgcatcc ggagcatcag gcacaacttt aattgccacg gcatgcgaca tcggagtgat
     5641 cgcaggaacc ggtcctgata acaaaacacg caactgcatc ctggccttct ccaggttcaa
     5701 cacttcttgt gtcagtggca caggctgagc cgtgaataac gcctgaattt catagttcgg
     5761 tctgtcgttg caatcctctt ttgtcggtac atatttccag tcaccaaccc acgactcccc
     5821 ctgagagtcc gtaacgcctt ttttcacgta gcgatatcgc cacgcaactg gctctgcttc
     5881 cagtgatgcc agtgcgatac gaaacacatt ggcaagcagg ctgtttgacg attgtttatc
     5941 gtgcgccgcg tcgctcagga aaccagtgat gaatgattta atttccgcgt tttctctggt
     6001 aatagtggtc atttgttaat ccttaaaact ttatgccctg gcgcaaaagc acgtgttttg
     6061 tctttgctta ttcgccagcc atccttgcgc gcctcttttg cacagccagc ccatgacgta
     6121 cctatatact caccgaagtc tggcactgga tatacacctt ccgtacactg gcggcagtca
     6181 caatagagat gcattgtgta acttgcggca atagccatat cactctcctt taccatgaag
     6241 catggcggca cgacaggcgt tccagcctct cacctctgca atagcggcaa cagcatcgac
     6301 cgcgtacatg ctaagaggat taggcattgg tttttcttcc ggtactactg gaacgggggg
     6361 agcggcgtag acctcaataa ttccattatc aatatgccat tctccatcct tgaggtagtc
     6421 acttgtgccg tcaacttgct gttctgcaat gtggaatgca cctattggtt ttgcttccag
     6481 cgatgccaga gcaattcgtg ccagttcttc cgcttcttct gctggaagga caacgttgct
     6541 acccggtccg tatgtttcgc gccactgttt gattgtcagc agtcgctctt tggtaatagt
     6601 ggtcatgccg tagccccttc ttgatatttt tcaaaccaga acacaaccgg gtcagatttc
     6661 atttcaacca atcccatacg aaccagcgcc ttgcctttcc cggacgcaag gaattcacga
     6721 cgaccatcac tgataattcg ccgataatct tccaggctac tgcaatgctt gtgcagattg
     6781 catgggtggc atgccggaac catgttggat atatcgtcac ggtcctggtg aagcatattt
     6841 ccagcaaaac gaatgaccgg ttttacatgg tctgcatgcc acttttcgcc aagctcgcag
     6901 ccgcaataag cgcagcgacc gccgaacttc atgcgcagtt ctgcacgttg ttttttcgtc
     6961 agtgccatat caatctcctt tagtgcgcaa gtggtttttc cagcggtttt gcgccgcgct
     7021 gggctttttg caacaactgt gccccatcac cccgcaacac cccgtcaacc tcactcgtct
     7081 tacttagtcg gacctcataa cctcgtgcgc cacggtgcgg tttatatgag tcccagtctc
     7141 cccacggtgc tttctctgcc gcctcacgca gtgcctggta gttaatcttg ctcactggtt
     7201 gcctcctgac tttcagcctt cagcaccatg cgggagccat cgtccatttc ccacataatt
     7261 tcaccacctt ctgccatgac cagatgccag acaagctgag ccgcctcatt ggttacatcg
     7321 cgaccgggat cattaccgac acgaagactg gttccgtcgt caagattacg catttttgcc
     7381 agcacgatag tttttgaaaa cggagaaaaa cccagctgga gtcttgctgc attactcatc
     7441 tttccacctc acttcagtct catgttgttg tatcgttcat gactcaacag ggcccaggtc
     7501 agccctttat tccggctcag taaccgccag tccggaccaa cattaatctt gatatacccg
     7561 aatccctttt ccttcagttc atggtaattc ctctccccgc gacgaaaccg gttaacgagg
     7621 tccaccgcct tccgctggca ggctggcggt accccgggac agctcagcag accgggaacc
     7681 agctctttca tcttcatgac gcattcctta cctgttcagg gcttccggct gtatgccacc
     7741 ccctgacagc atgccagacg gccgtgagcg ggatattcat ctgctctgca cagagcatca
     7801 tgacgtccag cccgtcagcg ctctccacat cgttaatccc tgctttctgc cacagtctcc
     7861 agagttcaga gttttcatca tcgctgagtg tgataccccg ccccggacgc acatcaattc
     7921 ctgaaatacg gcgccgtgca gcaacgtcca cactggaaag gccaaaataa aacgccatga
     7981 gctcaatcga tccccccagt gccagagcgc gatcgattcg ctgcaggcgt ttctgttcag
     8041 tcctggcctg ctgcagcatg cgtacaaggt tattgtggtt aatatccagc cgcagcactg
     8101 acacctctga atttgacaga tagtgaattt cctcaatact cagcccctgc agcatctgca
     8161 tttcttcccg ggaaagcccc agcgcttcac agcggcgcag atagccactt ttgagatcca
     8221 tcaccagttg catcaacagt ccgttggtgg cctgtgacaa gttattactc atacctcacc
     8281 tccgcccgat atcaggcttt ccggtctcag atcccgggaa tcaacaccgt gattcagcgt
     8341 tgcctttttc ccctcgttgt atccctgcca gcgcgcatgg tcaccgccat tgcagtcctt
     8401 tacctcccgg aagacgacca ttccctcaaa ttgctggttc tgcagccacg tcttcattac
     8461 acgcgtctca tcttctgtga gccggaatgc atccaccaca tcacgcaccc cataaaccca
     8521 gccgtcacga aacatcgcag ctcttgcacg acgggttgag tgttttaatt tccggctctg
     8581 ggttgagaga tactcttttg tcgccttttg catctgccgg cagagaacat caaaggcata
     8641 cgccgccaca accggacgct ccccaaaacc ataaaatgtg acaatccgcc gccagtatcc
     8701 tccggttgtg atacgccaat ccaggtatgt atgacatccg aatgcccctg caacaacatt
     8761 cataaggcgc cccatccata ccggaacttt ttccgcttct gacggcgctc cctttgatga
     8821 tgaggttttt atcaccgaaa tatcagcatc cagttcggtg ataccatgct tccgcatcag
     8881 cgcctgtgcg cgggaaagag cctgagctgc ctcatgggga ttactgttat tccggctcag
     8941 cgccagcagt tttcttatcc gtttaatcag ttgctcctga cgtgctgcat cattattcat
     9001 cacgcccctc cacgttctct tcttccacct ggcgctgcag cccccgcaga cgacgcaaca
     9061 ccacaagcag acggagtaac ttgccggcat gctcatcatc cagcagtggt ggctcttctt
     9121 cagtactgcc accaatcagc agtgcatcaa gagaaaccgg attaacccgc agaacctcct
     9181 caccggtcag cctgacaaga aatgccatga ccggagaaca attcgcctct gccgcgccga
     9241 atcccgcaga cagttcgctt tccctgtcca tagccagttc atcgacacag cctgtgtgtg
     9301 acgccagctc ccatgccagg tgaaatgcct cattctgcag atattcgata tcatccagtt
     9361 caccaacact gaattccggc tcactgaaaa tttcaccgct gctctgctga gcaccctctt
     9421 ccggtgacgt taccggcaga tccgcagcat cacgggtatc gccatcctct tcgtaatcgc
     9481 cgctgtatgt ttcttcacca ccatacatat ccggctggac ttcctttctg cggcgttctt
     9541 cgcgttgttc agtaactgaa ctctccgcct ttccaacagg ggattgctcc gtattcatga
     9601 ctgtgtcatg gtcagccggt gcggaaatca cagactgctc ccttccgctg tccttctcca
     9661 ccgtcactgc cgacgtaccg tgagtcacag gctctggcat cgcatcactg tctgcgggaa
     9721 tgagcaccgg ttcaggttca ccaaaatgat gacgtcggtt acgttcccgc ggatccagtt
     9781 caagcagcca gcggtcataa tccagcaccg gatgtggtaa cgctttcagc agatcaccaa
     9841 tcagctcatc ccggaacatc tccagtgacc agagttccgg tgaattgaat ttgctgcagc
     9901 attcaccaaa cacgcggtca aaatcccccc cgttctctgt tgccaccgca aactgtcccc
     9961 agatacgttc cgcatcctgc cggagagaca gtaaagccct gacctgagga ctccccattc
    10021 ctgattccag aagttcgggg atccacggat acaggtattt cagggtgttc tccatacggc
    10081 tgatactgga tgcatccacc ggtaatccct gctcagtcaa aagggatgac aactctcgca
    10141 tggacacact tttgcttaac tgctcttcat aaatcatccg tgctttatgg ataccccggg
    10201 ccttttcaat aaacgtcagt tcgcccctga cttcattctc cgccaggtga ccaatgacgc
    10261 actgaagccg tcccggccat ggcttgaaaa gcacatgaat acggtagaaa cgctgatcac
    10321 cggtctcttc ccacagctct ttcaggatct gatatcgcgt gttaccgcca tcgctgaaaa
    10381 tatagatatc cggctcactc tcaggtatcc gggtgacttt gggcaccgta tccagtccac
    10441 gggagaaaat tgaactttta atatcgtcgt accgtggatt acgcgtggtc ctcgggttat
    10501 ccgggttggg gcgcagttga tccagcgtca gaatcatcgg tgtttccccc agcgccacca
    10561 cgttacctgc cgtgctggcc tgacggcccg gttgcatgat ggcagccccc agatcaacgg
    10621 aatgatttcg tttaattgac atacgcattt cctccggcac ggtcaaaatt ccatccacgt
    10681 tcaaacggtg aaaacagggt gtagcggccg tcgaagttca cccgtatcgt tccggttggt
    10741 ccctgccgct gtttgctcac aatgatttca gcaacgccct tatccagcgt gccggagtta
    10801 taaacctcgt cacggtaaat aaacataatg aggtcagcat cctgctccag cgccccggaa
    10861 tcacgcaggt caccgttatt ggggcgttta tccgcccgct gctccacctg acggttaagc
    10921 tgagacagtg ccagtaccgg gcatcccagc tctttaccta acgccttaag cgcgcgggat
    10981 atttccgcaa tttcctgcgt tcggttctcc tggtccggag aacgaatcag ctggaggtaa
    11041 tcaaccatta tcagggaagg tctgccatac agacggacat aacgacgggc gctggcacgc
    11101 agcgatgacg gcgtctggaa ggaggtgtca tcaattatca ggcggtttcc atctccgatg
    11161 aatgacgctt cattcatgaa gcgtcccatg gttgccgtga ctctcgccca ctcttcatca
    11221 tccatcatgc tgctgcgaag acgtgtcagc tccaccctgg agagcattga caggacacgc
    11281 agaatcagct gctcccggga catttcgata ctgaaaagaa ataccgtttc atcggtccgg
    11341 ttacacaggg cattaagaca gaaggtaagg cccagcgctg ttttccccat agacgggcgt
    11401 gcagccagaa gaatgaggtc gccgggctgc agaccacagg tcatctcatc cagttcatca
    11461 aatcccgtgg gcgtgcccgt catcccgccg gcgttcagtc cctcttccag ttgacgcagc
    11521 gccagctcca tagccgaggt gagactgact tcacatcgct catgcaacat gccagtctgt
    11581 gaaagtgaaa agagccgacg ttcagcggat tcaagaacag tgcgtggtgt cgcatccggt
    11641 gcggccacat ccgtaatcag cgcattcccc gtctcctgca actgacgcag catgctcttt
    11701 tcagcaacaa tacgggcata agcgataata ttggctgcag aaggcgtatt tttgctgagt
    11761 tcagccagat aagcgaaacc acccagtgcc tcaatattgt ttgttttctc aatatgctca
    11821 ctcagcgtga tgaggtcgaa cggctgaccg ctggttgcca gctctgacat ggtgcggaat
    11881 atcacccggt gtgcggccag agaaaaatcc tccccccgga gcatcagtac cacctcatcc
    11941 cagcgttcat tatcgagcat caggccgccc agcacggcct gctctgcatc aacggagcgt
    12001 ggaatatagc gttcagacat acgccacctc ccgggcaaac ggagcctgtt tcacagcaaa
    12061 cccaccatgc aaaaaaacga aaacgggtgc cggacgaaca gttgcagggc ataaaaggcc
    12121 cataactttc tgtacatttt ccgaccggta agtttgccga ccccggtcag aaggtacgta
    12181 cagcatattt gcgctcataa tcaacatgtt tacgaagaga aataccttat gaacacaaat
    12241 catcagcaac tgccgtccgg cattgataac aaccatgatg aggatggcaa catggcaaaa
    12301 tattatgaaa actggaaagt cggaggtatg agcaccgacg atgtggaacg gtgcaaaacc
    12361 ttctttgacc agggaacaga aatctttccc ggagctgacc ggaagctcgc ttaccgactt
    12421 tgctggctcg gacactggct gattaacagc cgccgctggt tttttctgga cgataacagt
    12481 atcagcgttc aacaagccgt taagtttgct cttattcatc aacatcactg gctccctgca
    12541 acggttgaac agatgacacc cgctgagatg tcccttgctc tgacagatta ctgggctgac
    12601 tactggacac agccgactga gccggagaag gcgctgattc acatgactga ctggcagtat
    12661 caaaaacttt gttcaaatcg aaaaaaggta aatggtgacc aatgaagcgt tcaatttgag
    12721 cacctctgcg acaaatagca ttggactgag aatcaagtct ggttatctga gattccagtt
    12781 cctcacactg tttacgaagg tttgacagat tcagccctgc ctcatcaagc agggctttaa
    12841 atccttcgtt aagcagcgct gtaaatactt tgcaacgttc cctgacgtcc tcttccggtg
    12901 ctttattctc aaaaacttcc cgggcaataa aatccagtaa ggcatgacag gctgactcaa
    12961 cgggtgaaag cgtattacgg gttgtggtat tgctgctgtt attcataggg ttaatccttc
    13021 acgtcatatt gtccggtatt gataacgtcc ataagccagg gtaacggagc tgcatggttc
    13081 tcgcttaacc tgaatcactg acgtattccc ccgttagcca gtagcccgtc aaacgcctgt
    13141 ttccactcag gaaatagttc acaggccagg ctgtgcatcg tgacaagcgc ggaatcactg
    13201 cgacgtgtgg attctttttc gagacgatgt acgggttgac cagccgcatg cccctgcttg
    13261 taaatattca ggtcgtaaat ctgtgtggaa agaagggaaa ccgcaccttc aggtaactgc
    13321 ctgctgtaac gaccggtctg aataattccg gccagctcat ccagcgtctt cctggccagt
    13381 gccgtataat ccatgcagtt aaccagcgtg cgtactttcg gcaggggtat ccccagttcc
    13441 cggtaaggca gcaggttctc cataacgttg acagtgccgc gaataaactc gcgcacatca
    13501 gggagtaccg gattgaccat cccgacaacc gaatcagtgg ccgacagcac aatcatttcg
    13561 agcatgacag aacgcgcacc ctgagaatca actataatga catcgtagtt gttcagaaaa
    13621 agcggatgct gcaggacatt ccggaggcgc agacgaccat ccggggcgtg aagcatcgca
    13681 gtttttaact gctcatgagg gtcattggag actatcagat cgagtccgtc gatagctgtg
    13741 cgggaaatga tgttatcggg ataactgagg tcagccgtgc gcatcagcaa ctcaaaaaga
    13801 ccggctgacg cttcatatgt cagcggaaag atgttgcttg ccgtgggctg agcatgatct
    13861 ccgtcaatca gcagggtacg gatacctgca tcggcgagaa aaccagccag gttagcggat
    13921 tgtgttgatt taccttcgcc accttttgtg gagacaacgg gaagtatttt catatctgcg
    13981 tcctatcgac agttatgtga tcggatgcgc atgaaagact tcgacaacaa cttgacatgc
    14041 ccgtaactat ctgaatttta tgacatgggg attgaaaatc cccgtgtcct tggttcgatt
    14101 ccgagtccgg gcaccaaatt catataaacg gacctccacg gaggtccgtt tttcgtttca
    14161 ggacgccacg atttaagcgt tctgcctaca aatcaattct accgaactca accaga
//
LOCUS       DBBGLQ010000114        14079 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.114, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000114 DBBGLQ010000000
VERSION     DBBGLQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.114"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            77..292
                     /gene="essD"
                     /locus_tag="ACIYSB_004371"
     CDS             77..292
                     /gene="essD"
                     /locus_tag="ACIYSB_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGC5431282.1"
                     /translation="MKSMDKITTGVAYGTSAGSAGYWFLQWIDQVSPSQWAAIGVLGS
                     LALGFLTYLTNLYFKIKEDRRKAARGE"
     gene            426..770
                     /locus_tag="ACIYSB_004372"
     CDS             426..770
                     /locus_tag="ACIYSB_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGC5431283.1"
                     /translation="MTQNHELVVKGTRNFENKIAVILTLRDKKRFAGEIFDLHINLER
                     QEGATLEFYESEAIKRAKTLFRDVAAGLCEGDELSPEKRPIILEAQNVWITYKGKLPG
                     RITGSLKTPPKW"
     gene            complement(736..1008)
                     /locus_tag="ACIYSB_004373"
     CDS             complement(736..1008)
                     /locus_tag="ACIYSB_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431284.1"
                     /translation="MDNSTISLQELLDCISRLRDDVNALTVAFSYLAFSIPREQMQPT
                     LASLLLESRNPKWSQEQQNSFKWLAALLEEKYAGEITISAESSENQ"
     gene            1114..1647
                     /locus_tag="ACIYSB_004374"
     CDS             1114..1647
                     /locus_tag="ACIYSB_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGC5431285.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1864..2049
                     /gene="rzoD"
                     /locus_tag="ACIYSB_004375"
     CDS             1864..2049
                     /gene="rzoD"
                     /locus_tag="ACIYSB_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGC5431286.1"
                     /translation="MRKLKMMFCVMMLPQVVVGCTSKQSVSQCVKPPPPPPAWIMQPP
                     PDWQTPLNGIISPSERG"
     gene            complement(2140..2433)
                     /gene="iss"
                     /locus_tag="ACIYSB_004376"
     CDS             complement(2140..2433)
                     /gene="iss"
                     /locus_tag="ACIYSB_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HGC5431287.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEARVYCSK"
     gene            complement(2797..2988)
                     /gene="ylcI"
                     /locus_tag="ACIYSB_004377"
     CDS             complement(2797..2988)
                     /gene="ylcI"
                     /locus_tag="ACIYSB_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGC5431288.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRASTSIKSDE"
     gene            3377..3925
                     /locus_tag="ACIYSB_004378"
     CDS             3377..3925
                     /locus_tag="ACIYSB_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGC5431289.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRRKVEELRQASETDLQPGTIDYERYRLTKAQADAQELKNA
                     REEGLVLETELFTYIFQRVAQNISGILVRVPQTLQRKYPDISPAHLDAVKTEIAKASD
                     VASESGENVWRWIDDFRRAEGG"
     gene            3897..5825
                     /locus_tag="ACIYSB_004379"
     CDS             3897..5825
                     /locus_tag="ACIYSB_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGC5431290.1"
                     /translation="MISDELRAANSTGAITTGLLALKIPVPLTTVQWADQHYYLPKES
                     SYTPGRWETLPFQVAIMNSMGNDLIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVPALLELAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYC
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKAAPTPADESTSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            5809..6015
                     /locus_tag="ACIYSB_004380"
     CDS             5809..6015
                     /locus_tag="ACIYSB_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGC5431291.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            6012..7604
                     /locus_tag="ACIYSB_004381"
     CDS             6012..7604
                     /locus_tag="ACIYSB_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGC5431292.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMEEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDAETTQ
                     LFRTRFKAVSPKRVDTPGYGLGNRFLRAGVEVDRHGRAVAYHICEDDFPLSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPEGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSSWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAASRRPEEETTPRET"
     gene            7594..9099
                     /locus_tag="ACIYSB_004382"
     CDS             7594..9099
                     /locus_tag="ACIYSB_004382"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGC5431293.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGMLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTAVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHLSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPEEVRQDMQQRIDAAHRMFAEKVAMYTGLSVDVVTGTEAAVFEGQSGIEAGLA
                     DELVNASDAISVMTTALYTHDTGGTMPQLTATEAAAQENQRVMGILTSKEAKGREQLA
                     TMLAGQQGMSVEQAREILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVENARAVLA
                     AGSGKAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            9136..9483
                     /locus_tag="ACIYSB_004383"
     CDS             9136..9483
                     /locus_tag="ACIYSB_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGC5431294.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSPTPALTPLMLD
                     EATGKLVVWDGQKAGGAVGILVLPLEGTETVLTYYKSGTFTTEAIRWPDSVDEHKKAN
                     AFAGSVLSHAALP"
     gene            9541..10569
                     /locus_tag="ACIYSB_004384"
     CDS             9541..10569
                     /locus_tag="ACIYSB_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGC5431295.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNSRQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEYAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSPAN
                     NITQASGKKWSEHDRDTFDPAHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAADGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            10621..10983
                     /gene="gpFI"
                     /locus_tag="ACIYSB_004385"
     CDS             10621..10983
                     /gene="gpFI"
                     /locus_tag="ACIYSB_004385"
                     /inference="COORDINATES: protein motif:HMM:NF025366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGC5431296.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEAED
                     KNQESDENERHLMTYSYSTGGDEMSTVRVLKTLHVAGKHPHDDSDMEIVVTGMTVRVD
                     PVNVAELVLEGLVTDESY"
     gene            10979..11332
                     /locus_tag="ACIYSB_004386"
     CDS             10979..11332
                     /locus_tag="ACIYSB_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGC5431297.1"
                     /translation="MNIRDNGFDTAIARADETIRGHMGTSATITSGEQPGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            11344..11919
                     /locus_tag="ACIYSB_004387"
     CDS             11344..11919
                     /locus_tag="ACIYSB_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5431298.1"
                     /translation="MAIQGLEQAVENLSRISRTAVPGASAMAINRVATTAINQSSSQV
                     ARETRVPRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMSAPLKQA
                     FDENVNRIRRERLPKELSYALKQQLRIVIKR"
     gene            11916..12311
                     /locus_tag="ACIYSB_004388"
     CDS             11916..12311
                     /locus_tag="ACIYSB_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGC5431299.1"
                     /translation="MKNTDIRTAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            12319..13071
                     /locus_tag="ACIYSB_004389"
     CDS             12319..13071
                     /locus_tag="ACIYSB_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5431300.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            13085..13507
                     /locus_tag="ACIYSB_004390"
     CDS             13085..13507
                     /locus_tag="ACIYSB_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGC5431301.1"
                     /translation="MFLKTEQFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTQAISHAQN
                     VVYRLSGMYEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            13489..13923
                     /locus_tag="ACIYSB_004391"
     CDS             13489..13923
                     /locus_tag="ACIYSB_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGC5431302.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13916..>14079
                     /locus_tag="ACIYSB_004392"
     CDS             13916..>14079
                     /locus_tag="ACIYSB_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5431303.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAA"
BASE COUNT         3403 a         3283 c         4179 g         3214 t
ORIGIN      
        1 atagttacgc tgcggccttc tttttcccct tcctaataca agaactacgc aatccgttac
       61 tggcggaggc gttgctatga aatccatgga taagataaca acgggtgtcg cctatggcac
      121 ctcggcaggc agtgccgggt actggttttt gcagtggatt gatcaggtta gtccgtcaca
      181 gtgggctgct attggtgtgc tggggagtct ggcactgggc tttctgactt atctgacgaa
      241 tctgtacttc aagataaaag aggaccgtcg caaggcggcg cggggagagt aattcaatgg
      301 aacataataa tgaaaaatcc agagagcact ctggaaacag caaacattat tccattgctc
      361 attcattcgg cggctttcgt gatgtagtcg gacagggtgc tgagagagaa caggagagta
      421 atacaatgac tcaaaaccat gaactggttg ttaaaggaac ccgaaatttt gagaataaaa
      481 ttgcggtgat tttaaccctg cgtgacaaaa agcgttttgc cggtgagata tttgacttac
      541 acatcaacct tgaacgacag gaaggcgcca ctctggagtt ttatgagtca gaggcaataa
      601 aacgggcaaa gacattgttc cgggatgttg ctgcagggtt atgtgaaggg gacgagctgt
      661 cgccggaaaa gcgccccata attttagagg cgcagaatgt gtggataacc tacaaaggaa
      721 agctaccggg aagaattact ggttctctga agactccgcc gaaatggtaa tttcaccagc
      781 atatttttct tccagtaatg ccgccagcca cttgaaagaa ttttgttgtt cctgggacca
      841 tttggggttg cgtgactcaa gcagaagcga tgccagtgtt ggttgcattt gttctctggg
      901 aattgagaag gccagatatg aaaatgcaac agtgagggca tttacatcat cccgaagcct
      961 ggaaatgcag tcgagcaact cctgtagaga aatggtgcta ttgtccataa acaatcctct
     1021 ccattgtatt taactattcc ttgcctgatt caacaggccg ggacagataa acatatccag
     1081 ggttcagaaa ccgataaatc ctgataaata tccatgaacg caaaaatcag atacggcctg
     1141 tcggctgccg ttctggcact gattgccgtc ggtgcgcccg cgcctgatat tctcgaccag
     1201 tttctggatg aaaaagaagg taaccacaca acggcatacc gcgatgggtc cggtatctgg
     1261 accatctgtc ggggtgccac gatggtggat ggaaaacccg tttttcccgg tatgaaactg
     1321 tcgaaggaaa aatgcgacca ggtcaacgcc attgagcgtg ataaggcact ggcatgggtg
     1381 gagcgcaata ttaaagtacc actgaccgaa ccacaaaaag caggtatcgc gtcattctgt
     1441 ccctataaca ttggccccgg taagtgtttc ccgtcgacgt tttataagcg gcttaatgcc
     1501 ggtgatcgaa aaggcgcctg cgaggcgatt cgctggtgga taaaggatgg tgggcgtgac
     1561 tgccgtatcc gttcaaataa ctgctatgga caggttattc gtcgtgacca ggaaagcgca
     1621 ttagcctgtt gggggataga tcagtgagca gagtcgctgc gattatttat gctctggtta
     1681 tctgcatcat cgtctgcctg tcgttggcgg tcaatcatta ccgtgataac gccatagcct
     1741 acaaagaaca acgtgataaa aaagtcagtg agctgaagca ggcgaccgcc accatcgctg
     1801 acatgcagca gcgtcagcgt gatgttgctg cgctcgatgc aaaatacacg aaggagttag
     1861 ctgatgcgaa agctgaaaat gatgttctgc gtgatgatgt tgccgcaggt cgtcgtcggt
     1921 tgcacatcaa agcagtctgt cagtcagtgc gtgaagccac caccaccgcc tccggcgtgg
     1981 ataatgcagc ctccccccga ctggcagaca ccactgaacg ggattatttc accctcagag
     2041 agaggttgat cactatgcaa aaacaactgg aaggaaccca gaagtatatt aatgagcagt
     2101 gcagatagag ctgcccatat caatgggcaa ctcatgcaat tattttgagc aatacacccg
     2161 cgcttccagc ggagtataaa tgccaaaagt gatgaaaccg agcaatccat ttacgaatgt
     2221 ttgctgggtt tctgttttaa ccacattttc tgcaccacca caaatttttg ctgcatcgac
     2281 agttttcttc tgcccaattc ccgaaacgaa gaaatgatgg gtgatggttt cctttggtgt
     2341 tactgctgta ggtttgtttc caacagtaaa cgtctgttga gcacatcctg taataagcat
     2401 tgccagagcg gcagaaaaca acattttttt catcttatta tcctgcattg ttaaaaacgg
     2461 cagaatccta tgtgacaaca attaaacgat agttaaatgg attgatgaaa attaaaacta
     2521 cacaggtggc gctcagacga ttggagggag ttggggacac tcagaatcct gtagaatgaa
     2581 ataaaccggt ctatccgtcc attacccttt tagctgcgct gcatcgtcgc cgtattcccg
     2641 cattaaccat gaccgtagcc cgacggggaa ctcctctgcg cgagtgtgcg ggaataatca
     2701 aaaacgatgc acaccgggtt ttactgtgct gacagacgca gggttaccct catagtcgct
     2761 tttccggtgc gatggtggaa gaaaccggga tattcattat tcatcacttt tgattgatgt
     2821 agatgctctc ttttctgacg ttagtctccg acggcaggct tcaatgaccc aggctgagaa
     2881 attcccggac cctttttgat caagagcgat gttaatttgt tcaatcattt ggttaggaaa
     2941 gcggatgttg cgggttgttg ttctgcgggt tctgttcttc gttgacatga ggttgtcccg
     3001 tattctgtgt cgctgatttg tattgtctga agttgttttt acgttaagtt gatgcggatc
     3061 aattaatacg atacctgcgt cataattgat tatttgacgt ggtttgatgg cgtacacgca
     3121 cgttgtgata tgtagatgat aattattatc attttgtggg tcctttccgg cgatccgaca
     3181 ggttacgggg cgtcgacctc gcgggttttc gctatttatg aaaattttcc ggtttaaggc
     3241 gtttccgttc ttcttcgccg taacttaatg tttttattta aaacaccccc tgaaaagaaa
     3301 ggaaacgaca ggtgctgaaa acgggctttt tggcctctgt cgtttccttt ctctgttttt
     3361 gtccgtggaa tgaacaatgg aagtcaacaa aaagcagctg gctgacattt tcggtgcgag
     3421 tatccgtacc attcagaact ggcaggagca gggaatgccc gttctgcgag gcggtgggaa
     3481 gggtaatgag gtgctttatg actctgccgc cgtcataaaa tggtatgccg aaagggatgc
     3541 tgaaattgag aacgaaaagc tgcgccggaa ggttgaagaa ctgcggcagg ccagcgagac
     3601 agatctgcag cccggtacga ttgattacga acgctaccgt ctcacaaaag cacaggcaga
     3661 tgcgcaggaa ctgaaaaatg ctcgtgaaga agggctggtt cttgaaacgg aattatttac
     3721 ctacattttt cagcgagttg cgcagaatat atccgggatc cttgttcgtg ttccgcagac
     3781 actgcagcgt aaataccctg atatatcacc agcacatctt gatgctgtga aaactgaaat
     3841 cgcgaaagca tccgatgtgg cttctgaatc aggtgagaat gtgtggaggt ggattgatga
     3901 tttcagacga gctgagggcg gctaactcta caggagcgat aacgactggt ctccttgcgc
     3961 taaaaattcc tgtacccctg acgacagttc agtgggcaga tcaacattat tacctgccga
     4021 aagagtcgtc ttacacacca gggcggtggg aaacattgcc gtttcaggtt gccataatga
     4081 acagtatggg taatgacctg atccgcactg ttaatctgat taaatctgcc cgtgttggtt
     4141 atacaaaaat gctgttggga gtggaggctt attttattga gcataaatcc cgcaacagtc
     4201 tgctttttca gcccacggac tcagctgctg aagattttat gaaatctcat gttgagccaa
     4261 cgataaggga tgttcctgca ttgctggagc tggctccatg gttcggaaga aaacaccgcg
     4321 ataatacgct caccctgaag cgtttttcct ccggtgtggg gttctggtgt ctgggtggtg
     4381 cggcagcaaa aaattaccgt gaaaaatccg tggatgtggt ctgttatgac gagctttcct
     4441 cgttcgaacc ggatgttgaa aaagagggct cgccaaccct gctgggggat aagcgtattg
     4501 agggctctgt atggccaaaa tccattcgcg gctcgacgcc taaaatcaaa ggctcctgcc
     4561 agatcgaaaa agccgctaac gagtcggcac atttcatgcg tttttatgtg ccctgcccgc
     4621 actgtgggga ggagcagtat ctgaaatttg gcgatgaatc cacgcctttt ggccttaaat
     4681 gggagaagga cagccccgaa agcgttttct acctttgtga gcatcatggc tgtgtgatcc
     4741 atcagtctga gcttgaccag agtaacgggc ggtggatctg tgaaaacacg ggcatgtgga
     4801 cccgtgacgg cctgatgttt ttcagcgccc ggggtgatga aattccgccg ccgcgttcca
     4861 tcactttcca tatctggacg gcgtacagtc cgttcaccac ctgggtacag attgtctatg
     4921 actggctgga tgcactgaaa gatcccaacg gcctgaaaac ctttgtgaac accacgctgg
     4981 gcgagacctg ggaagaggcc gtgggcgaaa aactcgatca ccaggtactg atggataagg
     5041 ttgtgcgtta cacggcggcg gtgcctgccc gggtggttta tctgacggcg ggcattgact
     5101 cgcagcgaaa ccgttttgag atgtatgtct ggggatgggc tccgggagag gaagcctttc
     5161 tggtggataa aatcattatt atggggcgtc ccgatgagga agagacgctg ttacgtgtgg
     5221 atgcagcgat caacaaaaaa tactgccatg cagacggaac cgaaatgacc atttcccgtg
     5281 tctgctggga catcgggggg atcgatggag aaatcgttta tcagaggtca aaaaaacacg
     5341 gtgttttccg ggtgctgccg gtaaaaggcg catctgtcta tggcaagccg gtgatcacca
     5401 tgccaaaaac ccgcaatcag cggggcgtgt atctgtgtga agtggggaca gacaccgcaa
     5461 aagaaattct ctatgcccgt atgaaagccg ctcccacgcc tgcggatgaa tccacgtcgt
     5521 atgccatccg ttttcctgat gatccggaga ttttttcgca gacagaggcg cagcaactgg
     5581 tcgcggaaga gcttgtggag aagtgggaaa aaggaaagat gcgtctgctg tgggataaca
     5641 aaaagcggcg taacgaagcg ctggactgcc tggtgtatgc ctacgcggca ttacgtgtgt
     5701 ccgtgcaacg ctggcagctt gatctggctg tactggcaaa atcccgggaa gaagagacga
     5761 cccggccaac ccttaaagaa ctggcagcga agctgtccgg aggagtgaat ggttacagtc
     5821 gctgaactgc aggcgctgcg tcaggcgcgc cttgatttat taaccggtaa acgggtggtg
     5881 tctgtccaga aagatggtcg cagaattgaa tatacggcgg cttctctgga tgagcttaac
     5941 cgggcgatca atgatgcgga gtcggtactg gggacaaccc gacgtcgccg tcgtccgctg
     6001 ggagtgaggt tatgaaaaga acgcctgtcc tgattgatgt gaacggcgtt ccgcttcgtg
     6061 agagtctcag ctacaacggg ggcggcgcag gatttggcgg gcaaatggag gagtggttgc
     6121 caccggcgca gagtgccgat gcggccctgc tgcccgcgtt gcgtctgggg aatgcccggg
     6181 cagatgatct ggtgcgtaat aacggaatag cggctaatgc ggtggctctg cataaggatc
     6241 acattgtcgg gcatatgttt ctgatcagct accgtccgaa ctggcgctgg ctggggatgc
     6301 gggagaccgc agcaaaaagc tttgtcgatg aggtggaggc ggcctggtcg gaatacgccg
     6361 aagggatgtc tggcgagatc gacgtggaag gaaaacgcac gttcacggaa tttatccggg
     6421 aaggcgtggg tgttcatgcg tttaacggcg aaatctttgt gcagccggtc tgggatgcgg
     6481 agactacgca gctgtttcgt acgcgtttta aagccgtgag tccgaaacgg gtggacacgc
     6541 caggatacgg tttggggaac cgctttctgc gggccggggt ggaggtcgat cggcatggac
     6601 gggccgttgc gtaccatatc tgtgaggatg attttccgct ctctggtagt ggacgatggg
     6661 aacggatccc gcgtgaactt cccaccgggc gtccggccat gctgcatatt ttcgagccgg
     6721 ttgaggacgg gcagacccgt ggggctaatc agttttacag cgtcatggaa cggctgaaaa
     6781 tgctcgattc cctgcaggca acacagcttc agtcagccat agtgaaggcg atgtatgcag
     6841 cgacgattga aagtgaactt gataccgaaa aggcctttga atatatcgcc ggtgcgccgg
     6901 aggggcagaa ggataatccg cttattaata ttctggagaa gttctccagc tggtatgaca
     6961 cgaataacgt gacactgggc ggtgtcaaaa ttccgcacct tttccccggt gatgatctga
     7021 aactacagac tgcgcaggat tcagacaatg gattttctgc gcttgaacag gcgctgctgc
     7081 ggtatatcgc cgccggtctt ggcgtttcct acgaacagtt gtcccgtgat tactcgaagg
     7141 tcagttattc aagtgcccgc gcctccgcca atgagtcgtg gcgctatttt atggggcggc
     7201 gaaaatttat tgcggcccgg ctggccacgc agatgttttc ctgctggctg gaagaggctc
     7261 ttctgcgggg gattattcgt ccgccacggg cgcgttttga tttttatcag gcgcgttcat
     7321 cctggtcacg ggcagagtgg attggagccg gaagaatggc cattgacggg ctcaaggagg
     7381 tccaggaatc ggtgatgcgc attgaggccg gactgagcac gtatgagaaa gagctggcgc
     7441 tgatgggtga ggattatcag gacattttcc gccagcaggt cagggaatct gcagagcggg
     7501 aaaaagccgg actctcacgt ccggtgtgga tagcgcaggc gtatcagcag cagatagcgg
     7561 cgagtcgcag gccggaagag gagacaacac cacgtgagac gtaatctttc acacattatt
     7621 gccgcagcat tcaatgaacc gctgcttctg gagcccgcct atgcgcgggt tttcttttgc
     7681 gcgctcgggc gcgagatggg ggcagcaagt ctttcggtac cacaacagca ggtacagctt
     7741 gatgctcccg gaatgctggc tgaaacggac gagtacatgg ccggaggtaa acgaccggcc
     7801 cgtgtttacc gggtggtgaa cggtattgct gtactgccgg tgaccggcat gctggtgcac
     7861 cggctgggtg gtatgcggcc attttccgga atgacaggct atgacggcat tgtcgcctgt
     7921 cttcagcagg caatggcgga caccgctgtc cggggcgtac tgctggacat tgacagtccg
     7981 ggcgggcagg ccgcaggcgc gtttgactgc gctgacatga tttaccgcct ccgtcagcag
     8041 aagccggtct gggcactgtg caatgacacg gcctgttctg cagccatgct gctggcgtcg
     8101 gcctgctccc gacggctggt tacccagaca tcccgtatcg gctccattgg cgtgatgatg
     8161 agccatctca gctatgccgg tcatctggcg caggccggtg tggatatcac gctgatttat
     8221 gccggggcgc acaaggtgga tggcaatcag tttgaagcct taccggaaga ggtgcgtcag
     8281 gacatgcagc agcgcattga tgcggcacac cggatgtttg ccgaaaaagt ggcgatgtat
     8341 accgggctgt ctgtggatgt ggtcacggga acagaggccg ccgtttttga aggtcagtcc
     8401 ggcattgagg ccgggctggc ggatgaatta gtcaatgcgt cggatgccat cagtgtgatg
     8461 accacggcgc tgtacacaca tgatacagga ggcactatgc cgcaattaac tgcaacggaa
     8521 gccgccgcgc aggagaacca gcgagtgatg gggatcctga cgagtaagga agcgaaagga
     8581 cgtgaacagc ttgccacgat gctggcggga caacagggca tgagcgttga acaggcccgg
     8641 gagatcctgg ccgcggcggc accacagcag ccggtggcat ccgcgcagag tgaagccgat
     8701 cgcattatgg cgtgtgaaga ggcgaaaggt cgtgaacaac tggcggcaac gctggcggcg
     8761 atgccggaga tgacggtgga aaaagcccgc ccgatcctgg cagcttcacc gcaggcggat
     8821 gccggaccat cactccgtga tcagatcatg gccctggatg aggcaaaagg ggctgaggcg
     8881 caggctgaac agctggctgc ctgcccggga atgaccgtgg agaacgcccg ggctgtgctg
     8941 gctgcgggat caggtaaggc agaaccggtc tctgcatcca caaccgccat gtttgaacat
     9001 ttcatggcga accattcacc ggcagcggta cagggtggcg tgccacagac gtcagcagac
     9061 ggtgatgcgg acgtgaaaat gctcatggcc atgccatgaa gccagtgctg accatcaata
     9121 tgaggttttg acaatatggt aacgaaaacc atcactgaac agcgtgcgga agtacgtatt
     9181 tttgccggta atgatccggc tcataccgcc acaggcagca gcgggatttc ctcgccaaca
     9241 ccggcactga cgcccctgat gctggatgaa gccaccggga aactggtggt ctgggacgga
     9301 cagaaagccg gaggtgcggt tggcatactg gtactgccgc ttgaaggcac agagacggtg
     9361 ctgacgtatt acaagtcggg gacttttacg acggaggcaa tccgctggcc tgacagtgtg
     9421 gatgaacaca aaaaggccaa cgcctttgca ggcagtgtcc tgagtcacgc ggcgctgccg
     9481 taacacgtta tcaggccacc gcggtggcct gactgatttc tgaatgaaag gaactgattt
     9541 atgggattgt ttacgacccg ccagttactc ggttataccg aacaaaaagt gaaatttcgt
     9601 gcgctgtttc tggagctgtt tttccgccgt acggtgaatt tccataccga agaggtgatg
     9661 ctggacaaaa ttaccggaaa aacgccggtg gcggcctatg tctccccggt tgttgaagga
     9721 aaagtgctgc gtcatcgtgg tggtgaaacc cgcgtgttgc gtccgggcta cgtcaagccg
     9781 aaacacgaat ttaattcccg gcaggcggtt gagcgccttc ctggtgaaga tccggctcaa
     9841 ctgaacgatc cggcttaccg ccgtctgcgt atcattaccg ataacctcaa acaggaagag
     9901 tacgcgattg tccaggtgga agaaatgcag gcggtaaatg ccgtgctgta tggcaaatac
     9961 accatggaag gagaccagtt cgagaaaatt gaggtcgatt ttggcagatc gccggcgaat
    10021 aacattacgc aggccagtgg taaaaaatgg tctgagcacg atcgtgacac gttcgatcct
    10081 gcgcatgata ttgacctcta ctgcgaccag gccagcggcc ttgtgaatat tgccattatg
    10141 gacggtaccg tctggcgtct gctgaatggt tttaagctgt tccgcgaaaa actggatacc
    10201 cgtcgcggtt caaattcgca actcgaaacg gcagtgaaag acctgggggc ggtggtgtct
    10261 ttcaaagggt attacggtga tctggccatt gtggtggcga aaacgtctta tgtggcagag
    10321 gacggtaccg aaaaacgtta tctgccagac ggcacgctgg tcctggggaa tactgctgca
    10381 gatgggatcc gttgttacgg tgccattcag gatgcacagg cgttgtctga aggcgtggtg
    10441 gcctcttccc gttatccgaa acactggctg acggtggggg atcccgcccg tgaatttacc
    10501 atgacgcagt ccgcgccgct gatggtgttg ccggacccgg atgagtttgt ggtggtacag
    10561 gtgaaataat ccgtgagcgg gggcgaaatg cccccgtgtc ttttttcaca gggggctgat
    10621 atggcaacga aagagcaaaa tctgaaacgg cttgatgaac tggccctgat tctggggcgt
    10681 gagccggata tatccgggag tgccgcagag atagcgcagc gggtggcgga atgggaagag
    10741 gaagcagagg acaaaaacca ggagagtgat gagaacgaaa ggcatttgat gacgtatagc
    10801 tactccactg gcggggatga aatgagcact gttcgtgtac tcaaaaccct gcatgtggcg
    10861 ggaaaacatc cgcatgatga cagtgatatg gagattgttg ttacaggtat gactgtcaga
    10921 gttgatccag tgaatgttgc ggaacttgtc ctggaggggc ttgttacaga tgaaagttat
    10981 taatatcagg gataacggtt ttgatacagc cattgcccgg gctgatgaaa cgatacgcgg
    11041 gcatatggga acgtcagcca ccattacatc cggtgagcag cccggcgcag taatacgtgg
    11101 tgtttttgat gaccctgaaa atatcagcta tgccggacag ggcgtgcgcg ttgaaggctc
    11161 cagcccgtcc ctgtttgtcc ggactgatga tgtgcggcaa ctgcggcgtg gagacacgct
    11221 gaccatcggt gaggaaaact tctggataga ccgggtttcg ccggatgatg gcggaagctg
    11281 tcatctctgg cttgggcggg gcgtaccgcc tgccgttaat cgtcgccgct gaaaggggga
    11341 tgtatggcca tacagggtct tgagcaggcc gttgaaaacc tcagccgtat cagcagaacg
    11401 gcggtgcccg gtgcgtcagc aatggccatt aaccgcgtgg ccacaacggc gattaatcag
    11461 tcttcgtctc aggtcgcccg tgaaacccgg gtgccgagaa aacttgttaa agagcgatcc
    11521 agactgaaac gggctacggt cagaaatccg aacgcaaaaa ttatcgttaa ccgcggcgat
    11581 cttcctgtga ttaagctggg gatcaggatg ctgggacgtc gcccgaacag catactcaaa
    11641 gccggtcagc atcgttatca gcgggcattc atccagcgac tgaataatgg gcgctggcat
    11701 gttatgcaac gtctgccaga agccaggtat gcgaagggca atgacgataa gggaaggaaa
    11761 aagcgtaatc gtcttcccat tcaggtggtg aaaattccga tgtcggcccc actgaaacag
    11821 gcttttgatg agaacgttaa ccgtatccga cgcgaacgtc tgccaaaaga actgagctat
    11881 gcgctgaaac aacaactgag gattgtgata aagcgatgaa aaatactgat atccgtacag
    11941 ccgtactgga tgcactggag aagcatgaca ccggggcgac gctttttgat ggtcgccccg
    12001 ctgtttttga tgaggcggat tttccggcag ttgccgttta tctcaccggc gctgaataca
    12061 cgggcgaaga gctggacagc gatacctggc aggcggagct gcatattgaa gttttcctgc
    12121 ctgctcaggt gccggattca gagctggatg cgtggatgga gtcccggatt tatccggtga
    12181 tgagtgatat cccggcactg tcagatttga tcaccagtat ggtggccagt ggctatgact
    12241 accggcgcga cgatgatgcg ggcctgtgga gttcagccga tctgacttat gtcattacct
    12301 atgaaatgtg aggacgatat gccaacacca aatcctctgg caccggtgaa aggggccggg
    12361 accacactgt gggtttataa ggggaacggt gacccttatg cgaacccgct ttcagacgtt
    12421 gactggtcgc gtctggctaa agttaaagac ctgacgcccg gcgaactgac cgctgagtcc
    12481 tatgacgaca gttatctcga tgatgaagat gcggactggg ccgcgaccgg acaggggcag
    12541 aaatccgccg gagataccag cttcacgctg gcgtggatgc ccggagagca ggggcagcag
    12601 gcgctgctgg cgtggtttaa tgaaggtgat acccgtgcct ataaaatccg cttcccgaac
    12661 ggcacggtcg atgtgttccg cggctgggtc agcagcatcg gtaaggcggt gacggcgaaa
    12721 gaagtgatca cccgcacggt gaaagtcact aacgtgggta aaccttctgt agcggaagaa
    12781 cgcagcaaaa ttacgccggt cagtgcgatt aaggtgacgc cgacatccgg tacggtggca
    12841 aaagggaaaa caaccaccct gacggtttct tttgagccgg aaagtgcaac cgacaagacg
    12901 ttcagagcgg tttccgccga tccgtcgaaa gccaccatta gtgtgaaaga tatgacaatt
    12961 acggtaaacg gcgtggcgac aggtaaggtg cagatccctg tggtgagcgg aaatggtcag
    13021 ttcgccgcag tggctgaagt caccgttact gaagcgggcg ctgcagggta aacggaggta
    13081 atacatgttt ctgaaaacag aacaatttga acataacggc gtgaccgtca cgctttctga
    13141 actgtcagcc ctgcagcgta ttgagcatct cgccctgatg aaacggcagg cagaacaggc
    13201 ggagtcagac agcaaccgga agtttactgt ggaagacgcc atcagaaccg gcgcgtttct
    13261 ggtggcgatg tccctgtggc ataaccatcc gcagaagacg cagatgccgt ccatgaatga
    13321 agccgttaaa cagattgagc aggaagtgct taccacctgg cccacgcagg caatttctca
    13381 tgctcaaaac gtggtgtacc ggctgtccgg tatgtatgag tttgtggtga atgatgcccc
    13441 tgaacaggca gatgacgccg ggcctgcaga gcctgtttct gcgggaaagt gttcgacggt
    13501 gagctgagtt ttgccctgaa actggcgcgt gagatggggc gacccgactg gcgcgccatg
    13561 cttgccggga tgtcatccac ggagtatgcc gactggcacc gcttttacag tacccattat
    13621 tttcatgatg ttctgctgga tatgcatttt tccgggctga cgtacaccgt gctcagcctg
    13681 tttttcagcg atccggatat gcatccgctg gatttcagtc tgctgaaccg gcgtgaggct
    13741 gacgaagagc ctgaagatga tgtgctgatg cagaaagcgg cagggcttgc cggaggcgtc
    13801 cgctttggcc cggacgggaa tgaagttatc cccgcttccc cggatgtggc ggacatgacg
    13861 gaggatgacg taatgctgat gacagtatca gaagggatcg caggaggagt ccggtatggc
    13921 tgaaccggta ggcgatctgg tcgttgattt aagtctggat gcggccagat ttgacgagca
    13981 gatggccaga gtcaggcgtc atttttccgg tacggaaagt gatgcgaaaa aaacagcggc
    14041 agtcgttgaa cagtcgctga gccgacaggc gctggctgc
//
LOCUS       DBBGLQ010000115        13914 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.115, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000115 DBBGLQ010000000
VERSION     DBBGLQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.115"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            106..480
                     /locus_tag="ACIYSB_004393"
                     /pseudo
     CDS             106..480
                     /locus_tag="ACIYSB_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            992..1780
                     /locus_tag="ACIYSB_004394"
     CDS             992..1780
                     /locus_tag="ACIYSB_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HGC5431304.1"
                     /translation="MKSAIERRMEIANLVGVRGKVSVGDLAEYFGVSGATIRTDLRFL
                     ESMGHIVRSNGYVILNKGVIANFSAESRSRKETVVVGHEQDFAISSEKSNKTLFYSNG
                     NGWEQKLTEIIGSLEQSLFIGAGALTRHWAQTQKWNSGLILTNDIQLVTSRLFPASKM
                     TVIMPGGIVNSEQMMFEGGKTEENLKEYRVENAILEVDNFVPEEGFFAAHETDVRFLR
                     TLRTIAKKLVLIVRKENTSQIANFWIGDADFAEHFIYVSEIKTP"
     gene            1898..2371
                     /locus_tag="ACIYSB_004395"
     CDS             1898..2371
                     /locus_tag="ACIYSB_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGC5431305.1"
                     /translation="MANIVLVRIDERLLHGQIRITWGKYSGANTIIVANDEIAANPVL
                     QAPFKGAAGGEYVVLFRTIEHLIGSINKADERRRILILCKTPEDVARLVEGGVSFNEV
                     NVGNMHHNSERKQIDKNVSVSPVDIQAFKRIQLKGIKSIIQHMPESSRKDVFDCI"
     gene            2390..3160
                     /gene="agaW"
                     /locus_tag="ACIYSB_004396"
     CDS             2390..3160
                     /gene="agaW"
                     /locus_tag="ACIYSB_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGC5431306.1"
                     /translation="MYEAFLVALWAALCGIDKYDVALNFHRPLITGPVIGFILGDVQT
                     GLIAGAAMELAWLGLVPNAGSQPPDVTIGAIVGTAFAIKLGITPEASIGMAIPFAMAM
                     QALVIFLFTSFSPVMQKCDRYAEQGNASGIDRMLYFGLASRAMLYGVVAFLAVYYGTQ
                     AADFIIRYLPEKVIDGMAIAGKMMPAVGFAMLLRTMFNVKLLPHFFIGFILTTYFKLP
                     IIAVAIAFLCVAILDYFQSGSATEKKQEGILEDIHDDL"
     gene            3150..4016
                     /locus_tag="ACIYSB_004397"
     CDS             3150..4016
                     /locus_tag="ACIYSB_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGC5431307.1"
                     /translation="MISKATIDTDTNEKLTVSPDTEYQDKEISSTITKRDLWIVGFRG
                     LFMEGNFNYERMQAGGFCFSIIPALKKIHRNPHDFSLALKNNLSFYNAHPKLFTFPLG
                     LAIAMEENKEKPETINAIKAATMGPTGGIGDAIDHMTMMPLTLGVGAAISLQGSIAGP
                     FVFFILYQLYHFFVYFWLLFFGYKSGVAALGKLSEQADKLGRAANIIGLGVLGSMTAA
                     FVKVSTPLVVTAGAAHVNVQTDLLDRILPNALPLALVWFMFWLVQKGKSPSALIFGTL
                     ALGVVGHVIGIF"
     gene            4026..4463
                     /gene="agaF"
                     /locus_tag="ACIYSB_004398"
     CDS             4026..4463
                     /gene="agaF"
                     /locus_tag="ACIYSB_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGC5431308.1"
                     /translation="MIGIIVIGHINFASGLRSAVEAIAGEQPYIEYIDFIPGMSTEQL
                     EIHIRQAVKKCQTGEGILVFTDVAGGSPCNRATAVMTDYSDIRVITGSNLPMIANACL
                     ERDGTNLNELTSLILELGHHSIGLIDINSLETDDKPNDMNDGL"
     gene            4544..5578
                     /locus_tag="ACIYSB_004399"
     CDS             4544..5578
                     /locus_tag="ACIYSB_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HGC5431309.1"
                     /translation="MQHQSITQLMRKVYLWQKEHQVRWVIRSQGQRRYLKSTDWERGV
                     FWSGVAAAWRETQDEVYLNGLAEYTLNTGFRAGPLLNFADDQVCLQSYLEVYQTLGCD
                     DALQYAQKALEPMLTSEKRGREIWWWADALFMAAPTLAAFGANSQQPAYWEQMDRFWW
                     DAVDFLHDPETGLYYRDKRYMPLPEGEDVREQNGQKVFWARGIGWVLAAIPRLLAWLP
                     QNFPSRERYLAQYLLLAEQVVKYQSADGLWRTSLLDPEHFPEPESSASALFCYALAWG
                     INHGVLDDKYRPVVLRAWQGLTECVDEKGKLGWVQLPAYNPRRVDASHNIDYGAGVFL
                     LAGTEMLNLI"
     gene            5612..7069
                     /locus_tag="ACIYSB_004400"
     CDS             5612..7069
                     /locus_tag="ACIYSB_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HGC5431310.1"
                     /translation="MISPTQRPWLPHEHPGWKDYTLLFLQKLKREVLLRKPFISHNSE
                     IAELFAKNHLSFKEKCEQMAVYFIDSFFYYSRFQFCRAYLPGWPSEQGSESDAIEATA
                     RTLPLMAAWIHYQMTNQGKLDSYGKCVRQALKQAFICGTNPEHPGYWGKIKDYDQRIC
                     ECCDIALALWLVRNTVWKSYSASEQERILCWLQGVNNCKTVDNNWHLFIVLTQQVVLA
                     LSGKGENSEQRYARVKEFYVGEGWFRDGANGNFDYYNSWAFHYLLFWIDRINPDFDHQ
                     FITQSCAEFAKTFRYFFTPCGFPFFGRSISYRLAAPVALMANAIQSGRADGQLKRIIS
                     TLTHFFILHGAVQHGVITQGLFQADRRLMDGYSGSGSSLWSLRTLILMLYSGTECGLW
                     DTDEAPLEIETASFDFTIDAISARVTGIKETQEVIVTFAHDQYPDVPFKDGKLIMQGW
                     WPIVLESVSGRSTRPKNNLLRKGVTTFSSRLNLYL"
     gene            7114..8118
                     /locus_tag="ACIYSB_004401"
     CDS             7114..8118
                     /locus_tag="ACIYSB_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431311.1"
                     /translation="MKNYQQPCILFLTALMMISAFPAFAGGYIQFGSEYEYYPQKENI
                     AYHRLTSYNPYIRFSYRPQDSDWTYSGRILLKEYPYKNDYNNKVSTSQSGLYELYATN
                     YIKSGNFIFRPGIGFRIIPYDKANYYGERYERQLRILPQFDYLLTQDSRFYLNGFFYI
                     ADSKGSRKNDRTTDPYNNGECKASDANAFGFCSKKYSDWGYEFDAGVRHNINSTNSVA
                     LGVHTEFDDVTNNYDDQLIQGTLDYQYRTGKLTLGPYVRIALDRSIKLKSEKNPASNI
                     TLKQPYNRYGLRANYSFNGRWSMGAETYYQEEKMQDQSGNDMQSRQKWFYKLNVQYNF
                     "
     gene            8137..10536
                     /locus_tag="ACIYSB_004402"
     CDS             8137..10536
                     /locus_tag="ACIYSB_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HGC5431312.1"
                     /translation="MKKTNLAFSLLCLSMGSVHAQIATENVNLPVVKSTTTTSTQQQH
                     IIERMRDTWRQNFVPSGPAAPELSAEYVASLNKTANKLWKGIDKNTPAGQLWADTVLD
                     SESTSGRLKLGTTLYTVYQRLFTLAKAWATPGTDLYKNAQLYTVLKSALINLNQDYYN
                     DQTPEWGNWWNWELGISRSVNNTLVILYDDLPSTLIDKYNLATRHFVRDPRYLAEGSG
                     APYSTTKNAFTSTGGNRIDSAMVVFVRGLLANDPGEISAAVTSVPEVLNTVQSGDGFY
                     KDGSFIQHKDLPYSGTYGQVLLNGLGLIKNSVAGTPWDFSVEDNRRIYDVIRQAFLPL
                     LHEGKMPDAVNGRSISRKNGQDQDVGASVMNAIALFVNGAPPEEKHHIEQVLKAQLNS
                     KTTEYYHTHLPENLTSWQVITRIQQDSHLPPAPRTAGGKLYADMDRLIYQGTNYLAVV
                     AMHSNRTGSYECINNENLKGQRTSDGMTWLYLPNDDQYRDYWPVVDSRFLPGTTSAGE
                     QGWCDEQYRVTQLGRANIAWAGGNTLNKWASASMHLKVLTYSLRAKKSWFMAPHEMIM
                     LGSQISSSSPAVTTIANQKISGSAKVLVDGIVLQPGEERKATQSVVLNDKGNNIIWKP
                     LAGSSAQVSVKQRQGNWADIGTSSGKVSAQFLTIIQPHSTESDNHYAWVVFPSGSASP
                     SVNADITLLANDAKVQAVSLPGQQVIYANFWRSATVGGIHALTPMSLIMTPTTQGYQI
                     AVSSPRRDSRVSFQLPDNAIPFHISSDPDKRVSLNGDIVSVNMTNLRGSSYSFELSKN
                     K"
     gene            10546..13233
                     /locus_tag="ACIYSB_004403"
     CDS             10546..13233
                     /locus_tag="ACIYSB_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGC5431313.1"
                     /translation="MDKIIFCHRSAGYGSSLLLAVFSLSFISSSVNAAISSDCSGSSY
                     CTNKTIDANTAGYIDKSPVYFLGNTDLTVSASQAFNNNKGTYEFRENTHVKVNAESGL
                     NGGTYTLRGGSTPGKVEIDINASSGINNAKLTALAGSNGVVNANTEKAINGGSQIFNA
                     GTTLNINAADGVFNSTGLTFTDATLNLNASDAFSKNTMSSGKVGSVKGTSTVNINATG
                     GMSGGQLNIQDSSEVNVTGNGSVTGGTLLFTGSSVLNADTANAIAGETNNTNKQIFQS
                     GTTMNVNAATALSGGNQTFNDATLNVNASQGISGGYQILAKSSVLNTEADQAIIGGQI
                     KLQDNAVFNANGKSTITGGIQNFNDNSILNANGEDTLSGGRQNFSGNSILNGNSKGAI
                     SGNSKQIFSNNAIFNANISQAVTGGTSTTFKDDAVMNISAQHGVDGGNLLFDGNSTLN
                     INTADAINGGEQRFKGNSLINLNHENSLYNTTLKLEGNASVNINADNALNYTDHIVFS
                     KTYDTTTGSILDVNGHSLTIGSISGNDTNAVIKNSSAQDAILTTGLYDYGVFAGQSYQ
                     YDSFLQRGAMRADAVVSSALGDITRQAGLALIPQFADRLVPEPSGMAQNGQGIWVRTL
                     YGQQENQGDGATNAAWNGHVKGIQIGADLWPADEDSSHKLGVYVGYLDSNANVSGKTI
                     YTSNAQLGSYQLNTYATGLYWRYTATQGWYTNTTLQLARYSGQVESNNSTRKPDIHGR
                     GQILAFEAGYPRVLNEALTLVPRISVSWQKVKLDDYRYDDLNVNNDLDNQLTASAGAR
                     LYWNTMTISGVQWSPFLDAELNNQLTARDRNNVTVGSTGYQDSLLTSYNGSAAKISGG
                     VTVKLNDYTNYYIKVDYQNGLSERSEKTWQGTTGVSLYW"
     gene            13269..13412
                     /locus_tag="ACIYSB_004404"
     CDS             13269..13412
                     /locus_tag="ACIYSB_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431314.1"
                     /translation="MKYIMLFLMVIMLNACESAQNFKRPELSQQNCDTCNIDDADSPI
                     FDD"
     gene            complement(13580..13792)
                     /locus_tag="ACIYSB_004405"
                     /pseudo
     CDS             complement(13580..13792)
                     /locus_tag="ACIYSB_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4066 a         2844 c         3164 g         3840 t
ORIGIN      
        1 acctgaagca ctatatctca cgcgaagttt atatattgct acgaaatcaa aacgggcagg
       61 ttaacaacat cccgataacg tcttgactct tagaatggcg tcctaggaag tcagtataca
      121 ggactgaaat atcaacaaca tctttggcgt tacaggataa agcaaagtgt cagtcgacgg
      181 ggaaactgct aggataacag tcccatggag cgcttcttcc gcagtctgaa aacagaatgg
      241 gtgccgactg atggttacgt aggcaaggac caaaccaggc aacaaatcag cgtttatatc
      301 ctgaattatt acaatagtgt taggtctcac cattccaagt gtcaactcca cgatttttga
      361 ccgttaccgt cttatatcac tggcgctgac accaacggtg ggttgaagcc tgaagaatca
      421 gagaacagat accgttcttt ctgtaaaacc gtgaccaata ttacttggcc actacaataa
      481 ccctttatta tacaaatggt tgtgtattgt atcttaatct accctcaccg tttccccatt
      541 cccaacgcag atagcgcatg tccggaatag atatagacct ctatggccct cttctgattt
      601 aatcgactga tatatcggta ataatatcgt tttacaaaat gtcagggaat atgttgatac
      661 accagtcgat gatagccatc ccttagaaag gtagtcaggc ggaaccttct gtagtcaaaa
      721 caacaagaac ataggcagga tagcagcaaa tttgtccctg gtttgatgga agactggatg
      781 cggaaaataa tcccctagct tacatcaaca cataataaat tgatttatat gataaaaatt
      841 ataaaacatg gctaaagtta acattttttg tgacatgctc cacaattaat aacgatggtg
      901 gtttttaaaa cactggttta ggttcatatt gtagataatg aaagagaatg aaaaatgatg
      961 aaatgtttta acagcgttca atggagagct catgaaaagc gcgattgaac gtcgcatgga
     1021 aatcgcgaat ctggtcggag tgagaggtaa ggtcagtgtt ggagatcttg ccgaatattt
     1081 tggcgtatcc ggagcgacta tccgtactga cttgcgtttc ctagagtcaa tggggcacat
     1141 tgtccgctct aacggttatg tcattttaaa caaaggagtc attgccaact tttcagcaga
     1201 atcccggagt cgtaaagaaa cggtagtggt agggcatgaa caggattttg ccatatcatc
     1261 agaaaagagc aataaaaccc tattttattc aaatggcaac ggatgggagc agaagctgac
     1321 ggaaataatc ggcagcctgg aacaatcatt gtttattggt gccggagcgt taacccgaca
     1381 ttgggcacaa actcaaaaat ggaattcagg actaatactg acaaatgata tccagttagt
     1441 aacctccagg ttattccctg cgtctaaaat gacggtgatt atgcccggag gcatagtaaa
     1501 cagtgagcag atgatgtttg agggaggcaa aacagaagag aatttaaaag aataccgggt
     1561 agagaatgca attctggagg ttgataactt tgttcccgaa gaaggttttt ttgctgctca
     1621 tgaaactgat gtaagattct taagaacatt acgtactatt gcgaaaaaac ttgtacttat
     1681 tgtacggaag gagaatacct cacaaatcgc caatttctgg attggcgatg cagacttcgc
     1741 cgaacacttt atttacgttt cagaaattaa gactccctga tgtccaagta aatacagtat
     1801 ggagtaatat gaattatttc atactgtagt tttagttgta gctttatcat ttaaatcatc
     1861 attgatggtt cgttatcttt cggagaaaat ttaagtaatg gctaatattg ttctggttag
     1921 aattgatgaa cgtcttttac atggacaaat tcgaatcaca tgggggaaat attctggtgc
     1981 aaataccatt attgtcgcta atgatgaaat agctgccaac cctgtattac aggctccctt
     2041 taaaggcgct gctggcggcg aatatgtcgt acttttcaga acaattgagc atttgattgg
     2101 tagtatcaat aaagcggatg aacgcagacg catacttatt ctgtgtaaaa cgccagaaga
     2161 tgtcgccaga cttgttgagg gaggagtcag ttttaatgaa gtcaacgtcg gtaatatgca
     2221 tcataattcc gaacggaaac aaattgacaa aaatgtttcc gttagtccgg tggatattca
     2281 ggcctttaaa cgcattcaac taaaaggcat taaatcgata atacagcata tgccagaaag
     2341 ctcgcggaaa gatgttttcg actgtatctg attaaattta ggagtcatca tgtatgaggc
     2401 ttttttggtc gccttatggg ctgccctgtg cggaatagat aagtatgatg ttgcattaaa
     2461 ctttcatcga cctttaatta caggccctgt cattggattt attctgggcg atgtccagac
     2521 aggattgatt gcgggagctg caatggaatt agcctggtta gggttggtgc caaacgccgg
     2581 ttcacaacca ccagacgtta ccatcggagc gattgtcgga acagcttttg ctatcaaatt
     2641 gggtattaca cctgaagcat cgattggtat ggcgatcccg ttcgctatgg caatgcaagc
     2701 acttgtaatt ttcttgttca catcattttc accagtgatg caaaaatgtg atcgctatgc
     2761 cgagcaggga aatgcttctg gtattgatcg tatgctttat tttgggttgg caagcagagc
     2821 aatgttatat ggagttgtag cttttttagc agtttactac ggaacccagg cagccgattt
     2881 tattatcaga tatttaccag aaaaagttat tgatggaatg gccatcgcag gaaaaatgat
     2941 gcctgccgtg ggtttcgcca tgttgttacg tacgatgttt aatgtcaaat tactgccaca
     3001 ttttttcatc ggtttcattt taacaacgta ttttaaatta cctattattg ctgtcgcgat
     3061 agcatttctt tgcgtggcca tccttgatta tttccagtca ggaagtgcta ccgaaaaaaa
     3121 acaggaaggt atattagagg atattcatga tgatctctaa agcaaccata gacacagata
     3181 ccaatgaaaa actgactgtc tcacctgata ccgagtatca agataaagaa atcagttcta
     3241 ctatcacaaa aagggacctg tggatagttg gtttccgtgg tctttttatg gagggtaatt
     3301 tcaattatga gcgtatgcag gctggtggtt tttgttttag cattatccca gcgctgaaaa
     3361 agattcatcg taatccacac gatttttcac tggcgctcaa gaataatctt tcattttata
     3421 atgcccatcc taagcttttt acatttccac ttgggttggc aattgcgatg gaggagaata
     3481 aagaaaaacc agaaacaatt aatgctatca aagcagcaac aatggggccg acaggaggaa
     3541 tcggtgatgc cattgaccat atgaccatga tgcctttgac attaggtgtt ggcgcagcga
     3601 tatcgctgca aggtagtatt gccgggccgt ttgtattttt tattctgtac cagctctatc
     3661 acttttttgt ttatttctgg ctgctctttt ttggctataa atccggcgtt gcggctctcg
     3721 gaaaattatc cgaacaggca gataagctgg gacgagctgc caatattatt ggcttaggag
     3781 tcttgggttc tatgactgcc gcctttgtta aagtatcgac gccattagtg gtcacagcgg
     3841 gcgctgctca tgtgaatgtt caaaccgatc ttctggacag gatattgcca aatgcgctac
     3901 cactggctct agtctggttt atgttctggt tagtacaaaa aggtaaatcg cctagcgctt
     3961 tgatatttgg tacgttagct cttggagtcg tgggacatgt tattggtatt ttctaaggta
     4021 aaaatatgat tgggataatt gttataggcc atataaattt tgcctcaggg ttgcgttctg
     4081 ccgttgaagc tattgccggg gagcaacctt atatcgagta tatagatttt attcctggca
     4141 tgagtacaga acaactggaa atacatatcc gacaggcggt caaaaaatgt cagacagggg
     4201 aaggcatcct ggtattcact gatgttgccg gaggctctcc ttgcaaccga gcgacagcgg
     4261 ttatgactga ttattctgat atacgagtga ttactggcag taatttaccc atgattgcca
     4321 atgcctgtct tgaacgtgat ggcacaaatc ttaatgagct tacatcactc attctagaac
     4381 tggggcatca ttccattggg ctgattgata tcaacagcct tgagacagac gataagccaa
     4441 atgacatgaa tgatggcctc tgagtgaatg acaatctcta ggaactattt taattattaa
     4501 tatttcgcca cacaaaatct ggcatctaca aataggttat tttatgcagc atcaatcaat
     4561 tacgcaatta atgcgtaagg tctatctctg gcaaaaagaa caccaggttc gctgggtaat
     4621 caggagtcag ggacaacgcc gatatttaaa atcaaccgac tgggaacgcg gtgttttctg
     4681 gagtggcgtt gccgcagcat ggcgagaaac gcaggatgaa gtgtatttaa atgggcttgc
     4741 ggaatatacc cttaataccg gttttcgggc agggccactg ctgaattttg ctgacgatca
     4801 agtctgtctt caaagctatc tggaggtcta tcaaacatta ggttgtgatg acgctcttca
     4861 gtatgcacaa aaggcgcttg aaccgatgct gacaagcgag aaaagaggca gagaaatctg
     4921 gtggtgggcc gatgcattat ttatggcagc accaacatta gccgcattcg gcgctaattc
     4981 tcagcaaccc gcttattggg aacagatgga tcgcttctgg tgggatgccg tggattttct
     5041 tcacgaccca gaaacgggac tttactatcg tgacaagcga tatatgcctt taccagaggg
     5101 cgaagacgtc cgtgaacaga acgggcagaa agtattctgg gcgaggggca tcggctgggt
     5161 cctggcggca ataccacggc tcctggcctg gttaccccag aacttccctt cccgcgaacg
     5221 ctatctggcg caatatctac tgctagctga acaggtcgtg aaataccagt ctgcggatgg
     5281 cttatggcgt actagcttac ttgatcctga acattttcct gagcctgaaa gcagcgccag
     5341 tgcgttgttc tgctatgcgc tggcatgggg aattaatcat ggcgtgctgg atgataaata
     5401 ccgtcctgtc gtgctacggg cctggcaggg actgacagaa tgtgtcgacg aaaaggggaa
     5461 gttaggatgg gtacaacttc cggcttataa cccccgaaga gtggacgcca gccataacat
     5521 tgattatggc gcaggtgtct ttttgcttgc cgggactgaa atgttgaatc tgatttaaac
     5581 ctgtccggga atatgaggag tgatgagaga aatgatatca ccgacacaac gcccctggtt
     5641 gccccatgaa catcccggat ggaaggatta tacccttttg ttcctgcaaa aattaaagcg
     5701 tgaagtgtta ttacgtaaac cctttatttc acataacagt gaaatagctg agttgtttgc
     5761 gaaaaaccac ctttcgttta aggaaaaatg cgaacagatg gccgtgtatt ttattgattc
     5821 atttttctat tactcccgtt ttcagttttg ccgggcatat ttacccggct ggcccagtga
     5881 gcaaggttcc gagtccgatg cgattgaagc aacggcccga accttaccgc tgatggccgc
     5941 ctggatccat tatcaaatga caaatcaagg taagttagac agctatggaa aatgtgtccg
     6001 tcaggctcta aaacaagctt ttatttgtgg aaccaatcct gaacatccag gatattgggg
     6061 aaaaatcaaa gattatgatc agcgtatttg tgaatgctgc gatattgctc tggcgttatg
     6121 gctggtacgc aataccgtct ggaaaagcta tagcgcatct gagcaagaac gtattctttg
     6181 ctggcttcaa ggggtaaata actgtaaaac cgttgataac aactggcatt tatttattgt
     6241 gttaacccag caggtcgtac tggcgctttc cggtaaagga gaaaacagcg agcagcgcta
     6301 tgcacgggta aaagagttct acgtcggtga gggctggttc cgtgatgggg cgaatggaaa
     6361 cttcgattac tataattcgt gggcatttca ctatctttta ttttggatcg atcgtataaa
     6421 ccctgatttt gatcatcaat ttattacaca aagttgcgcc gaatttgcca aaacatttcg
     6481 ctatttcttt acaccatgcg gatttccttt ttttgggaga agtattagtt atcgccttgc
     6541 agcacctgta gcgctcatgg cgaatgccat tcaatcgggt cgggctgatg ggcaattaaa
     6601 gcggatcatt tctacactaa cccatttctt catactacat ggcgcggtac aacatggcgt
     6661 catcacccag ggattgtttc aagccgatcg tcggttgatg gatggctaca gcggtagtgg
     6721 cagcagttta tggtcactgc ggacattaat attgatgctt tattctggaa cagaatgtgg
     6781 cttgtgggat actgatgaag cacctctgga aattgaaact gcatcatttg attttactat
     6841 cgacgccatt tcagctcgcg tcacaggaat taaagaaacg caagaggtca ttgtaacatt
     6901 tgctcatgat cagtatcctg atgttccttt caaggatggc aaattaataa tgcaaggatg
     6961 gtggccaatt gttcttgaaa gtgtctccgg gcgctcaaca cgtcctaaaa ataacttatt
     7021 acgtaagggg gttacgactt ttagttcccg attaaattta tacctctgag aatataacga
     7081 cataaataaa tctaatatta ataaaggtgg cttatgaaaa actatcaaca gccctgtatt
     7141 ctgtttttaa cagcattaat gatgataagt gcgtttcccg cgtttgccgg gggatatatc
     7201 caattcggta gtgagtacga atactatcca caaaaagaaa acattgccta tcatcgactg
     7261 acgtcttaca acccgtatat tcgcttcagt tatcgaccgc aagactctga ctggacgtac
     7321 tccggccgta ttcttttaaa agagtatcca tataagaatg attataataa taaagtaagc
     7381 acctctcaat ctgggttgta tgaactatac gccactaact atattaaaag tggtaatttt
     7441 atcttcagac cggggattgg ttttcggatc attccttatg ataaagcgaa ctactatggt
     7501 gagcgatatg agcgacaact cagaatactg cctcaattcg attatctgct gacgcaggac
     7561 tcccgctttt acctgaatgg attcttttat atcgccgact caaaaggaag ccgtaagaac
     7621 gacagaacta ccgaccctta taacaatggc gaatgtaaag ccagtgatgc caatgccttt
     7681 ggtttttgtt ctaaaaaata cagtgactgg ggatatgagt tcgacgcagg agtccgccat
     7741 aacattaaca gcacgaatag tgtagcattg ggggttcata cggagtttga tgacgttacg
     7801 aataactatg atgatcagct aattcaggga acactggatt accaatatcg tacaggaaaa
     7861 ttaacgttag ggccatatgt ccgtattgct cttgatcgca gtattaaact gaaatcagaa
     7921 aagaaccctg cttcaaatat tactttaaaa caaccttata accgctatgg cttacgcgca
     7981 aattattcct ttaacggacg ttggtctatg ggtgccgaaa cctattatca ggaagagaaa
     8041 atgcaggatc agtctggcaa cgatatgcag tcgcggcaga aatggtttta taagctgaac
     8101 gttcagtata atttctgatt atagagggtg acatcaatga aaaagacaaa tttagcattt
     8161 tcgctattgt gcttaagtat gggcagcgta catgcacaga tcgctaccga aaatgtaaat
     8221 ctgccagtag taaaatcaac taccacaacg tcaacccagc aacagcatat tattgagcgt
     8281 atgcgtgata cctggcggca gaattttgtg ccttcaggcc cagcggcgcc agaattatca
     8341 gcagagtatg tggcaagttt aaacaaaacg gccaataaac tctggaaagg aatagataaa
     8401 aatacccccg caggccagtt atgggcagat accgtactgg atagtgaaag cacatcagga
     8461 cgcctgaaac tgggcactac tctttatacg gtataccaac gcctgttcac cctggcaaaa
     8521 gcctgggcta cgccggggac agatctttat aaaaatgctc agttgtatac tgtacttaaa
     8581 tcagcgctga tcaatctgaa ccaagactat tataacgatc agaccccaga atggggaaac
     8641 tggtggaact gggagttagg catttcacgc agtgttaaca atactctggt gatactttat
     8701 gatgatctcc cttccacgct gattgataaa tataatctcg cgacccgaca ttttgttcgc
     8761 gaccctcgtt atttagctga aggaagcgga gccccctact ccaccacaaa aaatgccttt
     8821 acgtcgactg gcggaaaccg cattgacagc gcaatggtcg tttttgttcg gggtctcctg
     8881 gctaacgatc ctggagaaat tagcgctgca gtaacttcag tacctgaagt gcttaacacc
     8941 gttcagtccg gagatggttt ctacaaagac ggatcgttta tccagcataa agatttacct
     9001 tatagcggaa cctatggcca ggttttgctg aatggtctgg gattaattaa aaacagcgtc
     9061 gcaggtacac catgggattt ctcagttgaa gataatcgcc gtatttatga cgtgatcaga
     9121 caagcttttt tacctttact tcatgaggga aaaatgcccg atgccgtcaa tgggcgcagt
     9181 atttcacgta aaaatgggca ggatcaggat gttggcgcat cagttatgaa tgccattgca
     9241 ttgtttgtta atggtgcgcc accagaagaa aagcaccaca ttgaacaggt attaaaagcc
     9301 cagctaaatt caaaaacaac ggaatattat cacactcact tgccagaaaa cttaacctcc
     9361 tggcaggtta ttacgcgtat tcaacaggat agccatttgc caccggcccc ccgaacagcg
     9421 ggcggtaaac tgtatgcaga tatggatcgt ctgatttatc agggtacaaa ctatctggct
     9481 gttgtagcga tgcattccaa tcgtactggt agctacgaat gtattaataa cgagaatcta
     9541 aaagggcaga gaacatctga tgggatgacc tggctgtatt tgcccaatga cgatcaatat
     9601 cgtgattact ggcctgtggt cgacagtcgg tttttaccag gcaccacctc tgctggcgag
     9661 cagggttggt gtgatgagca ataccgtgtg actcagttag gtcgggcaaa tatcgcctgg
     9721 gcgggtggca atactctgaa taaatgggca agcgcgagta tgcatttaaa agtgctgact
     9781 tattcactca gggcgaaaaa atcctggttc atggcaccgc atgaaatgat catgcttggg
     9841 agccagatat ccagtagtag cccggcggta acgaccattg ctaatcagaa aatcagtggc
     9901 tcggcaaaag tgcttgttga tggcatcgtc ttgcagcccg gagaagagag aaaagcaacc
     9961 caatctgttg ttttaaacga taaaggtaat aacattatct ggaagccatt agctggctca
    10021 agcgcacaag ttagtgtgaa acaacgtcag ggtaactggg ccgatatcgg cacctcatcc
    10081 ggtaaagttt cggcacaatt tttaaccatt atccagcctc atagcacaga atcagataat
    10141 cattatgcct gggttgtctt cccctcgggg tccgcatccc cttccgtaaa tgctgacata
    10201 acgctccttg cgaatgatgc aaaagtccag gctgtgtcgc taccaggtca gcaggttatt
    10261 tatgctaatt tctggcgttc tgcaactgtg ggaggcattc atgcattgac gccaatgtcc
    10321 ctgattatga cgccaacaac acaaggttat cagatagcag tatcttcacc acgtcgtgat
    10381 agtcgggtgt cattccaact gcccgataat gcaatcccat tccatatttc cagtgaccct
    10441 gataagcgcg tatctcttaa cggggacatc gtcagcgtga atatgaccaa tctacgcggc
    10501 agtagttatt cttttgaatt atctaaaaat aaataggatg cagccatgga taaaataatt
    10561 ttctgtcatc gctctgctgg atatggttct tccctgctat tggcggtgtt ttcattatct
    10621 tttatttcca gctcagtgaa tgcggctatt tcttctgatt gttcgggatc atcttattgc
    10681 actaataaaa caatagatgc caatactgct ggatatatcg ataaatctcc tgtttatttt
    10741 ttagggaata cagatcttac tgtttctgcc agccaggcct ttaataataa caaaggtacc
    10801 tatgagttca gagagaatac gcatgtaaaa gtcaacgccg agtcaggatt aaatggcggt
    10861 acatacacgc taagaggcgg aagtacacca gggaaagttg aaatcgatat caatgccagt
    10921 tctggtataa acaatgccaa gttaactgct ctcgctggta gtaatggcgt agtcaatgcc
    10981 aatactgaaa aagcaattaa tggcggtagt cagatattca atgctggaac aacactaaac
    11041 atcaatgcag ccgatggcgt atttaacagc acgggactga cattcacaga tgcgacactt
    11101 aatttaaacg catccgatgc ctttagtaaa aatacgatgt cctctggtaa agttggcagt
    11161 gttaaaggaa cgtcaacggt taatatcaat gccacggggg gaatgtctgg tgggcagtta
    11221 aacattcagg attccagcga ggtcaatgtg actggaaacg gtagtgtcac tggaggcacg
    11281 ttactgttca ctgggagttc agtattaaat gcggataccg ctaatgctat cgcgggagaa
    11341 acgaataata cgaataaaca gatatttcaa tctggtacga caatgaatgt gaatgcagcc
    11401 acggcactca gtggtggcaa tcaaacattt aacgatgcaa cattaaatgt caatgccagt
    11461 caaggtataa gtggtggata tcagatcctg gcaaaaagct cagtcctgaa tacagaagca
    11521 gatcaggcta ttattggagg acaaataaaa ttacaggata atgcagtatt taatgctaac
    11581 ggtaaatcaa caattaccgg ggggatacaa aattttaacg ataatagtat attaaatgcc
    11641 aatggcgaag ataccctgtc tggaggcaga cagaatttta gtggaaacag catcctgaat
    11701 ggcaacagta aaggggcgat ttccggtaac agcaaacaaa tatttagtaa caatgctata
    11761 tttaacgcca atatcagcca ggcagttact ggtggcacat ctactacatt taaagacgat
    11821 gctgtcatga acatttcagc acagcatggt gtggatggcg gaaatttatt atttgatggt
    11881 aattcgacat taaacattaa tactgcagat gccattaatg gtggcgagca gcgttttaaa
    11941 gggaattcac tgataaatct gaatcatgaa aactcgttat ataatacaac attgaaacta
    12001 gaaggaaatg ccagtgtcaa tataaacgcg gataatgctt tgaattatac tgaccatatc
    12061 gttttttcaa aaacgtatga cactacaaca ggcagtatac tggatgttaa cggacactcc
    12121 ctaaccattg gtagtatcag tggtaatgat accaatgcgg taatcaaaaa tagtagtgct
    12181 caggatgcca ttctgactac cggtttgtat gattatggtg tgtttgccgg gcaaagttac
    12241 caatatgatt cttttctaca gcgtggcgcg atgcgtgcag atgctgtcgt cagcagcgca
    12301 ttgggagata ttacccgtca ggctggactg gcgctaatac cacagtttgc tgacagactc
    12361 gttccggaac ccagtggaat ggcccaaaat ggccaaggga tttgggtacg taccctttac
    12421 ggacaacagg aaaatcaagg tgacggagcc acaaatgcgg catggaatgg ccacgtaaaa
    12481 ggtatccaaa ttggggctga tttatggcct gctgatgaag acagcagtca taaactgggt
    12541 gtttatgtgg gttatctcga cagtaatgct aatgtatcgg gcaaaaccat ttacacctcg
    12601 aatgcccagc ttggaagcta ccagctcaat acttacgcta caggtttata ctggcgttat
    12661 acagcaaccc agggctggta cacaaacacc actttacaac tagcccggta ttctggacag
    12721 gtagaatcga ataacagtac acgtaaacct gatattcatg gtcgtgggca gattctggct
    12781 tttgaagcgg gctatccgcg agtactaaat gaggcgctga cactggtgcc acgaatttct
    12841 gtatcctggc agaaagttaa attagatgac tatcgttatg atgatttaaa cgttaataac
    12901 gatctggata atcagttaac cgcctccgct ggagctcgtt tgtactggaa tactatgacg
    12961 atatccgggg tccagtggtc ccccttcctt gatgcagagc tgaataatca actgaccgcc
    13021 agagacagaa ataatgttac cgtaggcagt acgggatacc aggattcatt acttacatcc
    13081 tataatggtt ctgctgcaaa aataagcggc ggcgtgaccg taaagctgaa tgattataca
    13141 aattattata tcaaggttga ttatcaaaac ggtcttagtg aacgatccga aaagacctgg
    13201 cagggaacaa caggggtcag tttatactgg taactattgg ccattattaa ataatttaag
    13261 gagtggcgat gaaatatatc atgctgtttc tcatggtaat aatgctaaat gcatgtgaat
    13321 cagctcaaaa ttttaaacgt ccagaattat ctcaacaaaa ctgtgataca tgtaacattg
    13381 atgatgctga cagcccaatc tttgatgatt aatacgcaaa atccatgtta acacttacct
    13441 ttcggagcac cttcatcaac gtgctccgca aataaatatc ctccggctat gccggaggtt
    13501 tttcaaatgc gcctataagg ctctcttacc agccacgccc taacaggcgc acacgatctg
    13561 acatttgcat ccaacttcgt tacttacggc ccgtaaacgg gctgtccgga tagggaatcg
    13621 ataactgctc tcccatttta tcctcttcaa gctagtactt tatgtaatcc tgtattttcg
    13681 ccgtgttatt acccaccgta ccgacgtagt accctctgca ccagaactcc ctgttcctgt
    13741 atttgaattt caaatcccca aactgctcgt aaagcatcag actgaaccgc cccgggtttc
    13801 ctggagagtg ttttatctgt gaactcaggc tgccagatca tcgtttccga tggaagcata
    13861 ataagctttt tctgcttctg ccggtgggat atggcccagc ctttccagca atcg
//
LOCUS       DBBGLQ010000116        13896 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.116, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000116 DBBGLQ010000000
VERSION     DBBGLQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.116"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..366)
                     /locus_tag="ACIYSB_004406"
     CDS             complement(<1..366)
                     /locus_tag="ACIYSB_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HGC5431315.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGG"
     gene            complement(579..4802)
                     /gene="rpoC"
                     /locus_tag="ACIYSB_004407"
     CDS             complement(579..4802)
                     /gene="rpoC"
                     /locus_tag="ACIYSB_004407"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGC5431316.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(4879..8907)
                     /gene="rpoB"
                     /locus_tag="ACIYSB_004408"
     CDS             complement(4879..8907)
                     /gene="rpoB"
                     /locus_tag="ACIYSB_004408"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGC5431317.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            complement(9227..9592)
                     /gene="rplL"
                     /locus_tag="ACIYSB_004409"
     CDS             complement(9227..9592)
                     /gene="rplL"
                     /locus_tag="ACIYSB_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGC5431318.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            complement(9659..10156)
                     /gene="rplJ"
                     /locus_tag="ACIYSB_004410"
     CDS             complement(9659..10156)
                     /gene="rplJ"
                     /locus_tag="ACIYSB_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGC5431319.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            complement(10569..11273)
                     /gene="rplA"
                     /locus_tag="ACIYSB_004411"
     CDS             complement(10569..11273)
                     /gene="rplA"
                     /locus_tag="ACIYSB_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGC5431320.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            complement(11277..11705)
                     /gene="rplK"
                     /locus_tag="ACIYSB_004412"
     CDS             complement(11277..11705)
                     /gene="rplK"
                     /locus_tag="ACIYSB_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGC5431321.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            complement(11864..12409)
                     /gene="nusG"
                     /locus_tag="ACIYSB_004413"
     CDS             complement(11864..12409)
                     /gene="nusG"
                     /locus_tag="ACIYSB_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGC5431322.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            complement(12411..12794)
                     /gene="secE"
                     /locus_tag="ACIYSB_004414"
     CDS             complement(12411..12794)
                     /gene="secE"
                     /locus_tag="ACIYSB_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGC5431323.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            complement(13024..>13896)
                     /gene="tuf"
                     /locus_tag="ACIYSB_004415"
     CDS             complement(13024..>13896)
                     /gene="tuf"
                     /locus_tag="ACIYSB_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGC5431324.1"
                     /translation="VVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLE
                     LVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA
                     IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRK
                     LLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTP
                     FFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREG
                     GRTVGAGVVAKVLS"
BASE COUNT         3227 a         3786 c         3417 g         3466 t
ORIGIN      
        1 tcctccgtct aagtccacct caatcacggc atagctacca tacccggcat gaataacgtt
       61 gctttctggc agcgccaaaa tagttctttt taagtgtgtg cgcgctaagt tataacggtg
      121 ctggcagaag ggacgagatt ctttaggcgc gtggtacatt aacgattgtt tttcttcgtt
      181 cgtctgatgt tcataatctt ttgtgaagca gtgaagagag taggtaaccc aaaagcgata
      241 ctctaaaggc tctttgccct catgatgctg aacgtagaca gcctgatgag catcaagaaa
      301 tgataaatca cgaatatcac catccggatg tccaaaaggt cgccactttt ccacttcctg
      361 tttcattctc gttccttaag aaagttacac aggaatagta tcgaatcctt gccgagtttg
      421 actcaattag ttcagtcagt ttcaggatat tagtcatctc tacattgatt atgagtattc
      481 agaaattcct taaatattct gacaaatgct ctttccctaa actcccccca taaaaaaacc
      541 cgccgaagcg ggtttttacg ttatttgcgg attaacgatt actcgttatc agaaccgccc
      601 agacctgcgt tcagcagttc tgccaggctg gcagatgcgt cttctgcagt cacctgcggt
      661 gcagccggag cttcacccgc agcacggcga cgcatacgat cctggtggta cgcgtaaccg
      721 gtacctgccg ggatcagacg acccacgata acgttctctt tcaggccgcg cagttcgtcg
      781 cgtttgcccg caacggctgc ttcggtcagc acgcgagtgg tctcctggaa cgatgccgcg
      841 gagatgaagg actcggttgc cagagacgct ttggtgatac ccagcagatc gcgggagtaa
      901 gttgcaccca ctttgccgtt cgcttccagt tcgcggtttg cgatcttgac gcgagagtat
      961 tcaacctgtt cgccttccag gaagtcggag ctacccgcgt taacgatggt agctttacgc
     1021 agcatctgac gaacgataac ttcgatgtgt ttatcgttaa tcttaacgcc ctgcagacgg
     1081 tatacgtcct gtacttcgtt aacgatgtaa cgagtaacag catgaacacc acgcagacgc
     1141 agaatgtcgt gcggcgcttc cggaccgtcg gaaattacgt caccacgttc tacacgttca
     1201 ccttcgaaca cgttgagctg acgccatttc ggaatcatct cttcgtacgg atcgctaccg
     1261 tctaccgggg tgataaccag acgacgttta cctttggttt ctttaccgaa ggaaacgata
     1321 ccgctgattt cagccaggat tgccggctct ttcggacgac gtgcttcgaa caggtccgca
     1381 acgcgcggca gaccaccggt gatgtccttg gtaccgccgg attcctgcgg aatacgcgcc
     1441 agggtgtcac cagagctgat ctgtacgcca tcttccagct gaacaatcgc tttacccggc
     1501 aggaagtact gcgctggcat atcggtacct gggatcagaa cgtcgttacc ctgagcatca
     1561 acgattttca gtgccggacg cagatcttta ccacctgcgg tacgttctgc ggaatccaga
     1621 accaccagcg aagacagacc ggtcagttcg tcggtctgac gcgtaatggt ctggccgtcg
     1681 atcatgtcag taaagcgtac aaaaccgctt acttcggtga taaccggcat ggtgtgcggg
     1741 tcccagtttg caacggtttc gccgccagca acctgttcgc catcgccttt cgccagtacc
     1801 gcaccgtaag gtactttgta gctttcttta gtacgaccga attcgtcgat cagtttcagt
     1861 tcagtattac gggaagtgat aaccagttta ccgctggagt tcacaaccga cttcacgttg
     1921 ctgagcttga tgctaccttt gtttttcact tggatgctgg attcagcagc cgcacgagat
     1981 gccgcaccac cgatgtggaa cgtacgcatg gtcagctgtg tacccggttc accgatggac
     2041 tgtgccgcga taacaccgat tgcttcaccc ttgttgatga tgtggccacg cgccaggtca
     2101 cgaccgtagc agtgcgcaca tacaccaaag tcggtgtcac aagatacaac agaacgtact
     2161 ttaaccgcgt cgacagagtt ctcttccagc aggtcacacc actgttcgtg cagcagcgtg
     2221 ttgcgcggaa cgaggatatc agcagtaccc ggcttcagaa cgtcttcagc agttacacga
     2281 cccagtacgc gatcgcgcag cggctcttta acgtcaccac cctcgataac cggagtcatc
     2341 atgatacctt catgggtacc acaatcgtct tcggtaacca ccaggtcctg cgccacgtca
     2401 accagacgac gagtcaggta accggagttc gcagttttca gtgcggtatc cgccagacct
     2461 ttacgagcac cgtgggtgga gatgaagtac tggagtacgt tcagaccttc acggaagttc
     2521 gcggtgattg gcgtttcgat gatggagcca tccggcttcg ccatcagacc acgcatacca
     2581 gcaagctgac gaatctgtgc cgcagaacca cgcgcaccgg agtcggccat catgtagatg
     2641 ctgttgaagg aaacctgctt ctcttcctga ccgtcacggt taatcacggt ttcagtttgc
     2701 aggttatcca tcatcgcttt ggatacacga tcgttcgccg cagcccagat atcgataact
     2761 ttgttgtagc gttcgcccgc agttaccaga ccagactgga actgctcctg aatttcagca
     2821 acttctgctt ctgcctcgga gatgatttcg tgtttcttct ccgggatgac catgtcatcg
     2881 ataccaacag atgcaccaga acgcgctgca taggcgaagc cggtgtacat gatctggtcc
     2941 gcaaaaataa cggtcggttt cagaccgaga atgcggtagc aggtgttcag cattttggag
     3001 attgcttttt tacccagcgc ctggttgacg atggagtaag gcagaccttt cggtacaatc
     3061 atccacagaa tggcacggcc aacagtcgtg tctttcaggc tggttttcgc tactaattca
     3121 ccgttagcat ctttttcata ctcggtgata cgcactttaa cgcgcgcatg cagagaagcc
     3181 agaccagagc gatacagacg ttctgcttct ttcgggccag tcagcaccat gccttcgcct
     3241 ttggcgttaa cacagtcacg ggtcatgtag tacagaccca gtacaacgtc ctgagacgga
     3301 acgatgattg gttcgccgtt cgccggggac aggatgttgt tggtagacat catcagcgca
     3361 cgcgcttcca gctgggcttc cagcgtcagc ggtacgtgaa cagccatctg gtcaccatcg
     3421 aagtcggcgt tatatgccgc acaaaccagc gggtgcagct ggatagcttt accttcgatc
     3481 agtaccggtt caaatgcctg gatacccaga cggtgcagag tcggtgcacg gttcagcagt
     3541 accgggtgtt cgcggataac ttcgtccagg atatcccaaa cgacagcttc ttcgcgctca
     3601 accattttct tcgcagcttt aatggtggta gcaagaccac gcagttccag cttgccgtag
     3661 atgaacggtt tgaacagctc cagtgccatt ttcttcggca gaccgcactg atgcagacgc
     3721 aggtatggac ctacggtgat tacagaacga ccggagtagt caacacgctt accgagcagg
     3781 ttctgacgga aacgaccctg tttacctttg atcatgtcgg ccaaagattt cagaggacgc
     3841 ttgttagaac cggtgatcgc acgaccgcga cgaccgttat ccagcagggc gtctaccgct
     3901 tcctgcagca tacgtttttc gttacgtacg atgatgtccg gcgcagccag atccagcaga
     3961 cgtttcagac ggttgttacg gttaatgacg cgacgataca gatcgttcag gtcagaagtc
     4021 gcgaaacgac caccatccag cggaaccagc ggacgcagat ctggcggcag taccggcaga
     4081 acggtcagga tcatccactc tggtttgtta ccagactgaa cgaacgcttc cagcagtttg
     4141 atacgcttgg tcagcttttt acgcttggtt tcggagttgg tttcgttcag ctcttcacgc
     4201 agctgttcgc actcttgctc cagatccatg ctcttcagca gagcctggat tgcttccgcc
     4261 cccatcttcg cgtcgaattc gtcaccgaac tcttccagcg cgtccagata ctgctcttca
     4321 gtcaggatct gctgacgttc caggttggtc ataccgcctt cgataaccac ataggattca
     4381 aagtacagta cgcgttcgat atcgcgcagc ggcatatcga gcagcagacc gatacgggac
     4441 ggcagcgatt tcaggaacca gatgtgcgca gtcggggaag ccagttcgat gtggcccata
     4501 cgctcacggc gtactttagt ctgggtcact tcaacgccgc acttctcaca gatgacgcca
     4561 cggtgtttca ggcgcttgta cttaccgcac aggcactcgt aatcttttac cggcccaaag
     4621 atacgggcgc agaaaaggcc gtcacgttct ggtttgaacg tacggtagtt gatggtttcc
     4681 ggctttttaa cttcaccgaa agaccatgaa cggatcatgt ctggcgaagc cagagcaatt
     4741 ttgatcgcat caaactcttc ggttttagtc tgcgctttca gaaactttaa taaatctttc
     4801 acggatttgc tcccgtcgga gttagcacaa tccgctgccg ggttttaacc cgacagcagt
     4861 gacctgtttg agcgagaatt actcgtcttc cagttcgatg ttgataccca gcgaacgaat
     4921 ctctttcaac aatacgttga aggattctgg catgcccggc tccatctgat ggttgccgtc
     4981 cacgatgttt ttatacatct tggtacgacc gttcacgtca tcagacttaa cggtgagcat
     5041 ttcctgcagg gtgtatgctg cgccgtatgc ttccagcgcc cacacttcca tctccccgaa
     5101 acgctgacca ccgaactgtg ccttaccacc cagcggctgc tgagtaacca ggctgtaaga
     5161 accggtggaa cgcgcgtgca tcttgtcgtc gaccaggtgg ttcagtttca gcatgtacat
     5221 gtaaccaacg gttaccggac gctcgaactg ttcaccagtg cgaccatcgt acaggcggat
     5281 ctgaccggaa gtcggcaggt cgccaagttt cagcagctct ttaatttctg cttctttcgc
     5341 accgtcgaac accggcgttg cgattggcat acctttgcgc aggttttcag ccagacgcat
     5401 aacttcttca tcgctgaagg tactcaggtc aactttctga cgaacgtcag cgcccagatc
     5461 gtacgcacgc tggatgaatt cgcgcagttt cgcgacttct tgctgctgtt tcagcatggc
     5521 gttgatcttg tcgccgatac ctttcgcagc catacccagg tgagtttcga ggatctgacc
     5581 gatgttcata cgagacggta cgcccagcgg gttcagtacg atgtctaccg gcgtaccgtt
     5641 ttcatcgtaa ggcatatctt cgatcgggtt gatcttagaa attacaccct tgttaccgtg
     5701 acgacctgcc atcttgtcac caggctggat acggcgttta accgccagat ataccttaac
     5761 aatcttcagc acgcccggtg ccagatcgtc gccctgggtg attttgcggc gtttcgcttc
     5821 gagtttcttc tcgaactcgt gtttcagttc gtcatactgc tcagccagct gttccagctg
     5881 attttgtttc tcttcgtctg tcaggcccag ctccagccag cgatcgcgcg gcagtttgtc
     5941 gagcttctca gcttcaacgc caccggctac cagcacagca cggatacggc tgaacagacc
     6001 cgcttcgagg atctgcagtt cttcagacag gtctttcttc gcctgtttga gctgcatttc
     6061 ttcgatttcc agcgcacgtt tgtctttttc tacgccatcg cgagtaaaga cctgaacgtc
     6121 gataaccgta ccggatacac cgtttggtac gcgcagagaa gagtctttaa cgtcagaggc
     6181 tttctcaccg aagatcgcac gcagcagttt ttcttctggg gtcagctgag tttcaccttt
     6241 cggcgttacc ttaccaacca gaatgtcgcc accggtcact tccgcaccaa tgtaaacgat
     6301 accggattca tccagtttgg agagcgcagc ttcacccacg ttcgggatgt cagcggtgat
     6361 ctcttccgga cccagcttgg tgtcacggga cacacacgcc agttcctgaa tgtggatggt
     6421 ggtgaaacgg tcttcctgaa caacacgctc ggatacgagg atggagtctt cgaagttgta
     6481 accattccac ggcatgaacg ctacgcgcat gttctgacca agcgccagtt caccgaggtc
     6541 ggtggacgga ccgtctgcca gcacgtcgcc acgttcaacc ggttcaccca gagacacaca
     6601 cggcatctgg ttgatacagg tgttctggtt agaacgggtg tatttggtca ggttgtagat
     6661 gtcgatacct gcttcacccg gatacatctc gtcttcgtta actttgataa cgatacggga
     6721 agcatccacg tactgaacga caccaccacg tttagctacc gcagttacac cggagtcaac
     6781 ggcaacagca cgttccatac cagtaccaac cagcggctta tcagcgcgca gagtcggaac
     6841 ggcctgacgt tgcatgttcg cacccatcaa tgcacggttg gcgtcatcgt gttccaggaa
     6901 cgggatcagg gacgcaccga cggataccac ctgctgggtg gatacgtcca tgtagtcaac
     6961 ctggtcgcgg ctgaacaagc tggattcgcc tttgctacgg caagttacca ggtcttctac
     7021 gaagtggcct tcttcatcca agttggagtt cgcctgggcg ataacgtagt tgccttcttc
     7081 gatagcagac aggtagtgaa tttcgtcagt tacaacaccg tcggtcactt tacgatacgg
     7141 agtctcaagg aagccgtatt cgttagtctg tgcgtacacg gacagagagt tgatcagacc
     7201 gatgttcgga ccttcagggg tttcgattgg acatacgcga ccgtagtgag tcgggtgtac
     7261 gtctcgaact tcgaagcctg cacgttcacg ggtcagaccg cctgggccga gtgcggagat
     7321 acgacgtttg tgcgtaatct cagacagcgg gttgttctgg tccataaact gagacagctg
     7381 gctggaaccg aagaactctt tcactgctgc ggaaatcggc ttggcgttga tcatatcctg
     7441 tggcatcagg gtatccagat cgcccagaga cagacgctct ttcaccgcac gctctacacg
     7501 taccaggcca acgcggaact ggttttccgc catttcgcca acggaacgga tacgacggtt
     7561 gccgaggtgg tcgatatcat cgacttcgcc tttaccgtta cggatatcga tgagcttttt
     7621 cataacatca atgatgtcgt ctttgctcag gataccggaa ccttcgattt cttcgcgcag
     7681 cagagaacgg ttgaacttca tacgaccaac cgcagacaag tcataacggt cttcggagaa
     7741 gaacaggttc tcgaacaggc tttcagctgc ttcacgagtc ggcggctcgc cagggcgcat
     7801 catgcggtag atttctacca gtgcgctcag acggtcgtta gttgggtcga cacgtaaggt
     7861 ttcagagata tatgggccgt gatccagatc gttggtgaac agcgtttcga tacgcttgtg
     7921 accagactgg ctcagcttag ccagcagatc caggctcagc tccatgttcg ctgcgcagat
     7981 cagctcgccg gtagactcat caatatagtc tttagcaacc actttacctg cgatgtactc
     8041 aaccgggact tcgatcagtt tgacgtcgtc tttttccagc tggcgaatgt ggcgcgcagt
     8101 gatacggcgg cctttttcta cgtacacttt accgttagct tcgatgtcaa aagatgcggt
     8161 ttcaccacgc aggcgttccg gcaccagttc catctgcagc ttgttatcac ggatttcaaa
     8221 gataactttt tcaaagaaca ggtcgaggat ctgctctgtg gtgtagttca gggcgcgcag
     8281 aatgatggtc gcaggcagtt tacggcgacg gtcgatacgt acgaacaggt tgtccttcgg
     8341 atcgaattcg aagtccagcc aggaaccacg gtaagggatg atacgcgcgt tatacagcac
     8401 tttacccgaa gagtgggttt tacctttgtc ggagtcaaag aagacgcccg gactacggtg
     8461 cagctgggaa acgataacac gctcagtacc gttgataaca aaggtaccgt tgtctgtcat
     8521 gagcggaatt tcgcccatgt agacttcttg ttctttaatg tcttttacgg tgccttccgg
     8581 cgcttcgcgc tcatagatca ccagacgcag tttaacgcgc agcggtgcgg aataggtcac
     8641 gccacggatt tgacattcct ggacgtcaaa caccggttcg ccaaggcggt agctgacgta
     8701 ttgcagctcg gaattaccgc tgtagctctg aatcgggaat acggaacgga aagcagcttc
     8761 cagaccatac tgcccttcag gatcttgctc gataaatttc tgaaacgagt caagctggat
     8821 agaaaggaga taaggtacat ccagaacttg tggacgttta ccaaaatcct tacgaatacg
     8881 ttttttctcg gtataggagt aaaccatagg gttcctcagc tcgctgacaa gtcgacccat
     8941 ctgtccattg agcggacagt ttgtgcaaca ctattttgtt gaccggaaaa tggaacactt
     9001 tccgcaatgc ctgttgctat cacgcttaaa ccatttcatt gcgatttaca cagaacggac
     9061 gtcctgtcgc agtatattaa gtcgtcgata gaaacaagca ttgaaaggca cagcagtagt
     9121 caaacagtgt gaaacgctac tggcgcctta cagcgcaaaa aggctggtga ctaaaaagtc
     9181 accagccatc agcctgattt ctcaggctgc aaccggaagg gttggcttat ttaacttcaa
     9241 cttcagcgcc agcttcttcc agagcttttt tcagtgcttc tgcgtcgtct ttgctcacgc
     9301 cttctttcag agcagccggt gcagattcta ccaggtcttt agcttctttc agacccaggc
     9361 cagttgcgcc acgtactgct ttgataacag caactttgtt agcgccagca gctttcagaa
     9421 ttacgtcgaa ttcagttttt tcttcagcag cttcaaccgg gccagcagct acagctacag
     9481 cagcagcagc ggaaacaccg aatttttctt ccattgcaga gatcagttct acaacgtcca
     9541 ttacagacat agctgcaact gcttcaatga tttgatcttt agtgatagac atttaaattg
     9601 ttcctgaata tcagaataag tttatacgta agcgaatgcg ttaaaaagat aactgcgatt
     9661 aagcagcttc tttcgcatcg cgtacagcag ccagagtacg aaccagtttg ccagccgaag
     9721 cttctttcat ggttgccatc aggcgtgcaa ttgcttcttc gtaggtcggc agagttgcca
     9781 ggcggtcgat ctgagacgcc gggatcagct caccttcaaa ggcagcggct ttgacctcaa
     9841 attttgcatt cgctttcgcg aactctttga acagacgagc agcagcgccc gggtgttcca
     9901 tagagtatgc aatcagggtc ggaccaacaa acgcgtcttt caggcactcg aacggagtac
     9961 cttcaacagc acggcgcagc agggtgttac gaacaacacg catgtatacg ccagcttcgc
    10021 gacctgcttt acgcagttca gtcattttat ctacagttac gccacgggaa tccgcaacta
    10081 ctgcagacag cgcgcctttg gctacttcgc tgacttcagc aacaatcgct tgtttgtctt
    10141 gaagatttaa agccattagc tttgctcctg gatgtttgcc agagaaaatc tctggaactc
    10201 acttcactcc tccaaacgga gagagcgtct taattacggt gagcagaaac aagccagaat
    10261 ataaaagaat aatcttagcg ttctgtcacc gtctacgcag gggattaagt ttcttgcgaa
    10321 actcctgcgg tcttcgacgg aggcctggat aggccaggct ccaacgaaca aatcttttct
    10381 atccgtcgct attcagcttg tgacggcgtt ggcttccttc attcgcacca gtcacatagt
    10441 tagctatgct cctggcgact cactcagttg ccgccttgcc acaacccgaa tatctcggat
    10501 atacgtctgc attaagcatt atacgtgggg gtaagattgt agacaaaatc accgcccacg
    10561 taaaggcatt agtttacaga agcgctcagg ccagcctggt caactgcaac acctgcaccc
    10621 atggtggtgg agatgctaac tttcttgatg tacacgcctt tcgcctgagt cggttttgct
    10681 tttttcagcg caaccagcag agcttccagg ttttctttca gtttgtcagc gtcaaagtcc
    10741 actttaccga tggtggtgtg gatgatgccg tttttgtcgt tacggtaacg aacctggcca
    10801 gctttagcgt ttttaaccgc ttcagcaacg ttcggtgtta cagtacccac tttcgggttt
    10861 ggcatcaggc cgcgcggacc cagaacctgg cccagctggc caacaacgcg cattgcatcc
    10921 ggagaagcaa taacaacgtc aaagttcatt tcgcctttct tgatctggtc agccagatct
    10981 tccataccta ccagttctgc gcctgcagct ttagcagctt cagcgtttgc accttgggta
    11041 aatacggcta cgcgaacgga acggccagta ccgtgcggca gtacagttgc accacgtacg
    11101 ttctggtcag atttacgagc gtcgatgccg aggttaacag ctacgtccac gctttctacg
    11161 aatttagcag tcgccagctc tttcagcagt gcgatagctt cgttgatgtc gtactgtttg
    11221 gttgcatcaa ctttctcgcg gataacacgc atgcgcttgg tcagtttagc catttcttag
    11281 tcctccacta ccaggcccat ggaacgtgca gtaccttcga tggagcgagt catcgcttca
    11341 atgtcggcac cagtcatgtc ggcagctttg gtctgcgcga tttcctgcag ctgagcgcgg
    11401 gaaattttac ccactttgtc tttgttcggc ttaccggaac cagacttgat accagccgct
    11461 tttttcagca gaactgctgc cggcggggtc ttggtaacga aagtgaaaga acggtcagcg
    11521 taaacggtga ttactaccgg aatcggcaga cctttttcga tggaatcagt ttttgcgttg
    11581 aacgctttgc agaattccat gatgtttacg ccctgctgac ccagagccgg acctactggc
    11641 ggactcgggt tagccatacc agctgcaacc tgcagcttga cataggcttg tactttctta
    11701 gccattataa attcctcaag ttgggtaata acgcctctga gaggctcccc gtgatataaa
    11761 aaatcgtttt acgggcaagg cccataaaaa caaaaggcgc gaaattgtat tccaatctca
    11821 cgccttgtgc aacgattaaa tcgccgcttt tttgatcgct gggttaggct ttttcaacct
    11881 ggctgaagtc cagctctacc ggggtcgcac gaccgaagat agaaacagac actttcagac
    11941 gagatttctc gtaatccact tcttcaacaa caccgttgaa gtcagcgaac ggaccatcat
    12001 taacacggac catttcaccc ggttcaaaca gcgttttcgg acgcggctta tcaccaacct
    12061 gctgcaggcg gttcataatc gcatcgactt ctttatcgct gattggcgca ggacgatcgg
    12121 aagtaccgcc gatgaagccc atcacacgcg gtacgctgcg caccaggtgc cagctcgcgt
    12181 cgttcatcac catctgaacg aggacgtagc cagggaagaa tttacgttcg cttttgcgac
    12241 gctgaccgcc acggatttca accacttctt cggttggtac catgacttca ccaaacaaat
    12301 cttccatgtt gtgtaatttg atatgctcac gcagcgacgt tgctacgcgg ccttcaaaac
    12361 cggaaaacgc ctgaacgacg taccagcgct ttttaggagc ttcagacatc tcagaacctc
    12421 aggccagtga taaaggatac caggcgaacc agaataccat ccagtcccca caggatcagt
    12481 gacattactg cggtaaccgc agccacaatc agcgtggtgt gcaatgtttc ctggcgagtc
    12541 ggccaaatga ccttacggac ttcggtacgc gcttcacggg caaaagcaac ggtagcttta
    12601 ccttttgtcg ttaacagcgc gacaccaccc gctgcagcaa tcagaattac tacggccagc
    12661 gcacgcagcg gcagcataat gtcgcgataa agatagttgc cgacaatcgc caccaggagc
    12721 aatgccacca caacgaccca cttcatcgct tccaggccgc gcccgcttcc ttgagcttcg
    12781 gtattcgcac tcataaacca acctgtcaga agttttctac aaacattttc accccgcgat
    12841 cgcgaggcaa accaaatcga aacgcgtatt agcttttcgg attatacgcc ctcaacagag
    12901 cctgtctcag caatgattat gacaaagaaa atcactgatg agccaggttc tggtacgaaa
    12961 gcgtgcaaaa agggcatcaa atgatgccct tttagtgcgc attgcgtcaa atgttatcgg
    13021 caattagctc agaacttttg ctacaacgcc cgcgccaacg gtacggccgc cttcacggat
    13081 tgcgaaacgc agaccgtcgt ccatcgcgat cgggtggatc agggtaacaa ccattttgat
    13141 gttgtcgccc ggcattacca tctctacgcc ttccggcagt tcgatggtac cagtcacgtc
    13201 agtagtacgg aagtagaact gcggacggta gcctttgaag aacggagtat gacggccgcc
    13261 ttcatctttg gacagaatgt acacttcaga ttcgaacttg gtgtgcggct tgatggtgcc
    13321 cggcttagcc agtacctgac cacgttcgat ttcttcacgt ttgataccac gcagcagaac
    13381 acctacgttc tcaccagcac ggccttcgtc cagcagtttg cggaacattt caacgccagt
    13441 acaggtagac ttctgagtct ctttgatacc aacgatttca acttcttcac caactttgat
    13501 gataccgcgt tctacacgac cggtaacaac ggtaccacga ccggagatgg agaatacgtc
    13561 ttcgatcggc agcaggaacg gcttgtcaat cgcacgctct ggttccggaa tgtaagaatc
    13621 caggaagcca gccagttcca ggattttcgc ttcccactct gcgtcgcctt ccagcgcttt
    13681 cagagcagaa ccacgaacga tcggagtgtc gtcgcccggg aagtcgtact gagacagaag
    13741 ttcacgaact tccatttcaa ccagttccag cagctcttcg tcatcaacca tgtcgcattt
    13801 gttcaggaac acgatgatgt acggaacgcc tacctgacga cccagcagga tgtgctcacg
    13861 agtctgcggc atcgggccgt cagtcgcagc aactac
//
LOCUS       DBBGLQ010000117        13819 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.117, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000117 DBBGLQ010000000
VERSION     DBBGLQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.117"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            259..1089
                     /gene="yjiC"
                     /locus_tag="ACIYSB_004416"
     CDS             259..1089
                     /gene="yjiC"
                     /locus_tag="ACIYSB_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGC5431325.1"
                     /translation="MSMPLSNILQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1230..2003)
                     /gene="uxuR"
                     /locus_tag="ACIYSB_004417"
     CDS             complement(1230..2003)
                     /gene="uxuR"
                     /locus_tag="ACIYSB_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGC5431326.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSSDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(2218..3678)
                     /gene="uxuB"
                     /locus_tag="ACIYSB_004418"
     CDS             complement(2218..3678)
                     /gene="uxuB"
                     /locus_tag="ACIYSB_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGC5431327.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPGWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3759..4943)
                     /gene="uxuA"
                     /locus_tag="ACIYSB_004419"
     CDS             complement(3759..4943)
                     /gene="uxuA"
                     /locus_tag="ACIYSB_004419"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGC5431328.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5283..6626
                     /gene="gntP"
                     /locus_tag="ACIYSB_004420"
     CDS             5283..6626
                     /gene="gntP"
                     /locus_tag="ACIYSB_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGC5431329.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6799..7701)
                     /gene="fimH"
                     /locus_tag="ACIYSB_004421"
     CDS             complement(6799..7701)
                     /gene="fimH"
                     /locus_tag="ACIYSB_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGC5431330.1"
                     /translation="MKRVINLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVVIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIAVTFVYQ"
     gene            complement(7721..8164)
                     /locus_tag="ACIYSB_004422"
     CDS             complement(7721..8164)
                     /locus_tag="ACIYSB_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5431331.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDRGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNSG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8237..8767)
                     /gene="fimF"
                     /locus_tag="ACIYSB_004423"
     CDS             complement(8237..8767)
                     /gene="fimF"
                     /locus_tag="ACIYSB_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGC5431332.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPINAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8777..11413)
                     /gene="fimD"
                     /locus_tag="ACIYSB_004424"
     CDS             complement(8777..11413)
                     /gene="fimD"
                     /locus_tag="ACIYSB_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGC5431333.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLCSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLVGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11480..12204)
                     /gene="fimC"
                     /locus_tag="ACIYSB_004425"
                     /pseudo
     CDS             complement(11480..12204)
                     /gene="fimC"
                     /locus_tag="ACIYSB_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12241..12780)
                     /gene="fimI"
                     /locus_tag="ACIYSB_004426"
     CDS             complement(12241..12780)
                     /gene="fimI"
                     /locus_tag="ACIYSB_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5431334.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12845..13393)
                     /gene="fimA"
                     /locus_tag="ACIYSB_004427"
     CDS             complement(12845..13393)
                     /gene="fimA"
                     /locus_tag="ACIYSB_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGC5431335.1"
                     /translation="MKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTAID
                     SAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3392 a         3531 c         3293 g         3603 t
ORIGIN      
        1 ggcattatta atatagacat caatatagca acatcaaatt ccaattagta ataagatatg
       61 cttttaaatt gcatgaaaat aatttcaaat gagcgttttt tgatacttta gagtgtttcc
      121 taccctttaa tatcaccgtg tagagttgat atcactacaa cagaaacatg catgtcattt
      181 gtttacttct cattttacta cagaaaaatt tatctatttt tctaaacatc agtgaaatac
      241 tcataaaagg taactattat gtctatgcca ttaagcaata tccttcagag ccaaattatt
      301 acagataatc acttccttca tcatccaaaa gtcgacagtg agttaacacg taaatatgaa
      361 cgtgcacgcc tggatacgga aaatatctat cttctgcctc ttgctcgtgg caataaccat
      421 aattacgatg gaaaaagcgt tgtcgaaatt agaaaacttg atattagtaa agagtcctgg
      481 ccatttaatt atgtgacaga agcatgcaga gaatccgatg gtattacaac aacaggacgt
      541 atgctctatc gaaacttgaa aatcacctca gctttagacg aaatatatgg tggcatatgt
      601 aaaaaggcgc atgctgctac agaattagca gaaggtttac ggctaaattt atttatgaag
      661 agccctttcg atcctgttga ggactataca gttcatgaaa taacattagg ccctggttgc
      721 aatgttccag gttacgccgg aaccacgata ggatatattt caacccttcc agtttctcag
      781 gcaaaaagat ggaccaatga gcagccaaga attgatatct atattgatca gataataact
      841 gtctcagggg tagcaaattc ttccggattc gcccttgccg cattacttaa tgctaatatt
      901 gagatgggaa atgacccaat cattggtata gaagcctatc ctggtaccgc ggagatccat
      961 gcaaaaatgg gatataaagt tatccccggt gatgaagatg cgccactcaa acgcatgact
     1021 ctgcaaccgt catctcttcc agaactgttt gaattaaaaa atggtgaatg gaattatatt
     1081 ggtaaatagc cactgagaag aatgagtact aatattcagg ttatacagat atttaaacaa
     1141 taccctgcaa tttacaaagc aatactcatt gcgacttctt aatttaagaa agcgcaagga
     1201 acgtttaccc ttgcgcttat tataataagt cagacgtcga ctttatccag cggccatgaa
     1261 tcaaacagat agccgtcaaa ataaatatcg tcaacgttcg agaattccag cagacgttgc
     1321 ttaacgtttt ccagatgctg ccacattgcc agcttcgccg ctcgggcatc ttttttgatt
     1381 aacgcggcga ggatctgttt gtgatcgccc aaccactctt tgcgatacag gctgtcatcc
     1441 agatggctgt gcaactggat ccacattgga ttgttttccc gccactgcca ggactgacgg
     1501 aataactcca ccagcatgct gttatgcgtt gcttcggcaa tggcgagatg gaactgcata
     1561 tcaccgcttt cgctgctacc cggcgcgctg gaagccagtt cacgctcttc cagttgcaat
     1621 gcctgacgca ttttgacgat atcttcgcgg gtagcctgca aagcggcaaa ttccgcgatg
     1681 ttactttcca gtaactgacg cgcctgtaac agctcaaaag gacccgcatc attacagacg
     1741 ttggcatccg aactgtctgt gttgtggctg cctgagctat caagaacata gataccggca
     1801 ccccggcgca cttccaccag ccctttgatc tccagcatga tcagcgcttc acgtaccacc
     1861 gtccgcgtga catcaagcat ttctgcaatt tcacgctccg gcggcagccg ttcgccagga
     1921 ttgtacggcg tctttatgat cagatcgcgg atcatcgccc cgacttcctg gtaaggtctt
     1981 tgcgcagagg tggcagattt catggtgttg tgtccgttag gtgcggttaa tcaaattttt
     2041 gccggatgcg gcgtgaacgc cttatccggc ctacaacggg caattttacg gcccctgtta
     2101 atctcaaact ataccattag cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa
     2161 actggtcaac caaatatcag tttgattgac cagtaagtct gtagggtaat cagttagtta
     2221 cagcgcagcc acacactcgc gcgcaccgcg ttcgcacagc tgctgatatg ccgccgtcac
     2281 cgcgccaaca aagtcggcat tctgcggcag atcatcggca aaaataccgc tcaggcctag
     2341 cagcgctttc acgcggtctg cgccctgata ctgcgcgtta atcttctgga actcggccag
     2401 catcgggtcg accacgtcaa tggcattacc ctgctcatcc acgccctggg tgtaacgcat
     2461 ccagccagcc acgcccagcg ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg
     2521 caccgggtcc agcagacgct gcggtaactt ctggctgccg tccatcgcaa tctgccaggt
     2581 acggtgacgc agagacgggt tgctgaaacg ctcgatcagc agcgtcgcat aggcgttcag
     2641 gtccgtacct tccggcatcg acagcgttgg cgcttgttcc tgcatcatca gggcaaaggc
     2701 cgctttgcga taatccgggt tagtcatggt gtcggcaata gtttcatagc cgccgaggta
     2761 accgaggtac gccaggaaag agtggctacc gttcagcata cgcagcttca tcatttcgaa
     2821 cggcacaacg tctgcaacga actgtgcgcc cactttatcc cagcccgggc gaccattaac
     2881 gaaattatct tcaatcaccc actgacggaa cggttcgcag gcaatggcgc acgggtcgta
     2941 aacgcccagc tggtcagcaa tttcctgtaa ggtttctggc gtcgccgccg gaacgatgcg
     3001 gtcaaccatg gtgcacggga aggtgacgtt ctcttcaatc catgccgcta gctgcgggtc
     3061 acgcgcctga gccagcccca gtaccgcgac cttcgccaca tgaccgtttt cacgcacgtt
     3121 atcgcaggac atcaccgtaa atgctttcag ccctttttca cgacgcagac gcaaggcttc
     3181 gacgatgtaa ccaatcgcgg acttcggcgc agtcgggttt tccagatcgt gcttgatcag
     3241 cgggttattg agatccagtt gaccgcttgc cgcatcagcg cagtagcctt tttccgtgac
     3301 tgtcagagag acaatcgccg tttgcggacg cgccatcgcg ttgagaatac cttcgcagcc
     3361 gtcgatttcc gggtgcagcg cttctttcat cgaaccgata attttcagct cggtgctttc
     3421 cgcgcctttt tccgccacgg tgtacagcag ttgctgtttt ttcaggtttt cgatcagcac
     3481 gcggtcgttg cctggcatca ggttaacttc gcagataccc cagtcgctgt cggtgctttc
     3541 cagcagatga tgggtgtaca gcgcctggtg cgcacggtga aacgccccgc aaccgagatg
     3601 cacaatgcgt gattccagac gagaatgatc ccatgacggg cgggcaaccg gcagattgct
     3661 gtcaacaata gtagtcatcg taaactccag aatccgtctt cagagagtga cggcaaggaa
     3721 gaaggaaccg gcacggcgcg cagccatgcc ggtggatatt aacggctaaa gaaagcgcgc
     3781 tggatcgcca gttcgacacc gcgaacttcg gccaggcctt tcagacgacc aattgcggag
     3841 taacctgggt tggttttctt cttcaggtcg tccagcatct gatgaccgtg gtccggacgc
     3901 atcgggatca ggtcttcttt gccttccgct ttacgacggt gttcttcttc aacaatcgct
     3961 ttcaccactt cgtacatatc aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc
     4021 gggttatctt cacgcatggt ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc
     4081 ttgatcatat caaccagatc gttgtcagca cgcacgccgt aggaaccggt gcacatggtg
     4141 aaaccgttcg ccatgctatt tacggtatca accatccact gcatatcttc aatggtagaa
     4201 acaatgcgcg gcaggccgag gattgggcgc ggcggatcgt ccgggtgaac agccatacgc
     4261 acgccaactt cttcggcaac cggaataatc gccttcagga agacggcaaa gttttcgcgc
     4321 agtttggctt tgtcgatatc tttgtacagc tccaggtgct tacggaactg gtcgagggtg
     4381 tacccttctt ccgcgcccgg cagaccggca atgatgttac gggtcagacg cgctttgtct
     4441 tcgtcgctca tagtggcgaa gcgttcagct gcctgagcaa tttcttcttc ggtgtaatcc
     4501 gcttccgcac ctgggcgttt caggatatgc atttcgaatg cagcgaattc gatctggtcg
     4561 aagcgcagag ctttggagcc gtctggcagc acgtattcga ggtcagtacg ggtccagtcg
     4621 agcaccggca tgaagttgta gcacacggtg cgaataccgc actgcgccag gttgcgcaga
     4681 gtctgctgat agttagcgat ccactgctca tagttgccag tgtgggtttt gatatcttcg
     4741 tgaattggca cgctttctac gacagaccac accaggcctg catcttcaac gatagccttg
     4801 cgtttgagga tctcttctac ggaccatact tcaccgttcg ggatatggtg caaagcggta
     4861 accacgccag tcgcgcccgc ctgacggaca tcagctaaag aaaccggatc gtttgggccg
     4921 taccagcgcc aggtctgttc catgattttt cctctttcac atagtgggtt ccacagcaaa
     4981 gagacgcgga accctggagt tgtttaggta tccggatgaa caaccagacc gcagccgtgg
     5041 cgttgcatcg gatgagatgg cgtataagtt ctaccttttg gtaagacatg tcaacccgaa
     5101 ttctgaaatt ggttaaccac atcacaagaa tttcactctc ctggttgttg ctgcaaattg
     5161 cagcaaaagg taatcgatgg ttattgatca gaggatgtga cattcatcgc aacaatggtt
     5221 gaccaattta cataacatgt cgcccaaaat aacaacggtt caaccaggta aggtaaaacg
     5281 ttatgcatgt gcttaacatt ctctgggtgg tattcggcat tggtctgatg ctggtactga
     5341 atttgaagtt caaaatcaat tcaatggtgg ccttgttggt ggcggcgctg tccgtcggga
     5401 tgctggcggg catggatttg atgtcgctgc tgcacaccat gaaagcgggc ttcggcaaca
     5461 cgctggggga actggctatc atcgtggtgt tcggtgcggt catcggtaaa ttgatggtcg
     5521 actccggcgc ggctcaccag atagcgcata cgctgctggc acgtctcggt ctgcgttatg
     5581 tacagctgtc ggtgattatc atcggcctga ttttcggtct ggcgatgttc tatgaagtgg
     5641 cctttatcat gttagcaccg ctggttattg ttattgccgc cgaagctaaa attccgttcc
     5701 tgaaactggc gatcccggca gtagcagctg ccactaccgc acattcactg ttcccaccgc
     5761 agccgggtcc ggtggcgctg gtgaatgctt atggcgcgga tatggggatg gtttatatct
     5821 atggcgtact ggtgacgatc ccaagtgtca tctgcgcagg tctgatcctg ccgaagttcc
     5881 tcggcaatct tgagcgccca acgccatcat tcctgaaagc agatcaaccg gtagatatga
     5941 ataatctgcc ctctttcggc gtttcgattc tggtgccgtt gatcccggcg atcattatga
     6001 tctctaccac catcgccaat atctggctgg taaaagatac ccctgcgtgg gaagtggtta
     6061 actttatcgg ttcctcgccg attgcaatgt ttattgcgat ggtggttgca ttcgtactct
     6121 ttggcaccgc gcgcgggcac gatatgcagt gggtaatgaa cgcttttgaa agcgcggtga
     6181 aaagcattgc aatggtgatt ctgatcatcg gtgcaggtgg cgtgctgaag cagaccatca
     6241 tcgacaccgg catcggcgac accatcggca tgctgatgtc ccacggcaat atctcgccct
     6301 acatcatggc atggctgatc accgtactga ttcgtctggc gacgggtcag ggtgtggttt
     6361 cagcgatgac cgccgccggg attatcagtg cagcaatcct tgatccagca actggtcagc
     6421 tggttggcgt gaatccggcg ctgctggtac tggcgacggc tgcgggttcc aacaccctca
     6481 cccacattaa cgatgcctca ttctggttgt tcaaaggtta ctttgacctg tcagtaaaag
     6541 acacgttgaa aacctggggt ctgctggagc tggtcaactc cgtggttggg ctgattattg
     6601 tgctgattat tagcatggta gcgtaaaaga gcacctgagc ctgccattgg caggctctgt
     6661 tcggattgtc ggtaaagtgc gctttgttta tgccagatgc gacgctgacg cgtcttatct
     6721 ggcctacaaa gggctaacgt gcaggtttta gcttcaggta atattgcgta cctgcattag
     6781 caatgccctg tgatttcttt attgataaac aaaagtcacg gcaataatcg attgcacatt
     6841 ccctgcagtc acctgccctc cggtacgtgc gtaatttgcc gttaatccca gacttaccgc
     6901 cgaagtccct actgctccta acgataccgt gttattcgct ggaataatcg taccgttgcg
     6961 cgtcaactgt acgccgacgc cctgtgcagg tgaaaacgac gcggtattgg tgaaaatcga
     7021 gttgcccgca tctgcggttg tgccggagag gtaatacccc aggttttggc ttttcgcaca
     7081 ataaacggta agaggaatcg gcactgaacc agggtagtcc ggcagagtaa cggtgacatc
     7141 acgagcagaa acatcgcagc cgccagtggg caccaccaca tcattattgg cgtaaatatt
     7201 ccacacaaac tggaaatcat cgctgttata gttgttggtc tgtcgcaaaa taagcacggc
     7261 aattaatgag ccagctttaa tcaccacccc gcccgcactg ctcacaggcg tcaaataaag
     7321 cgccaccggc cacggcttat ccgttctcga attataaaca acccgcggcg tttcactggt
     7381 ggtcggaaat ggatagctac tgccactata ttttacggtc ccggaaaaat tagataacac
     7441 gccgccataa gccgagcctc gttgcagtgt gacataatct gtaatggttt ccgggtaatc
     7501 gttatggcaa aagatttgcg tcgaaagatc cacgaccagg ttttgcccca cattcacggc
     7561 gggcgcaagg tttacataaa cattggcgct gccaccgcca atagggatag cggtgccatt
     7621 ggcggtttta caggcgaatg accaggcatt taccgaccag cccatcagca gtacagcaaa
     7681 caggttaata actcgtttca ttacaatcat ctcttcggct tcagctgtag gtataggtga
     7741 tgctaatcac tgcctgaatg gttccctgag tggctccgcc atttaccgtc aatgctctga
     7801 cctgtaacgg gaagtgcgct gattgtgagg aatcatccac ctgaactgtt ttggttgcgc
     7861 cactattcaa tgtgttgcca ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc
     7921 cctggttttt ataatatccg gtactgtcgg ctgccccgct gaagctggca gtgaccctcg
     7981 acgttcccac cggacaatta gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc
     8041 cggcagacat cagactgaaa gaataaagat cgccgcgatc taccgtggca ttggtggtgg
     8101 aaaccgtgca cggtttggcg acgaccttac cgttcaccgt gatggtgaca tcggctgcct
     8161 gtatcgtcgc acttgcgagc gccaatattg ccgccaatac atacccacgt ttgcaccatt
     8221 tcatgagcat ctccagttac tgatattcaa gagtgaaggt agccgtggca ttgatatgcc
     8281 ccgcagtgac aggcacctgt gtcgccatta gccgggcgta aaaattcagc gtatttggtt
     8341 tacccggcgt cagggtcgtc cacgaaatcg cggacgatgg ggcattaatg ggtatctgat
     8401 tttgttgctc attcagaagc tgtattccca gtcccgcagc cgctgacacc gtattttcaa
     8461 gtgcaagcag gttggcattg tggctatctg caacaccggt aaacccaact tttacggcag
     8521 aaacggcatt accacagggt gacagcaaaa tacgaaatgg aacgacagga gtcgtcgcgc
     8581 caatgttgtt aaattgcttc gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt
     8641 cagcggccac actacagccg ttatctctga catagccgcg gatagtaatc gtgctatccg
     8701 cagccaaagc gtgacttacc gccagccaca aaaaagcgca cagaagataa aaaggtttgt
     8761 ttctcatcac gcccccttaa cgacattcag ctgatagctg ggttaataac tgctgctgac
     8821 tctctggtgg cagttgataa ttggcgacac agtgagcatt ttcctcttct ccccatttca
     8881 cctgaacttt tcccgctaaa ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa
     8941 tgccgctact ctggctactc tctgatgtca ccatcgcccc aaacggcagc ggcttattat
     9001 tgtgggttag cgtcatgagc agttttatcc caacgcgcgc tttaaactct gctcgcacga
     9061 tcgccccacg ggtgggaaca acgttagcga ccgcgttatc taaatcgacg ttatcagcca
     9121 gggtattggt atccagcgct actctgttct cccgatattc agtggcataa ggcagcacgg
     9181 cataaccgcg ccagtcggta cgcacccccg tctggttttc gacttttgca tcttttgcgc
     9241 caggcgcttt aacaagcacc accgtatcgt ttaacggctg ccccagcgtt acgccattgg
     9301 catgagccag taccccaccg ctgactccgt aatagagctg cttaatatca tcgctatggc
     9361 tgtaaccgat gttggcattg ccgtaaccac cgcgataatt cagcgtggcg tagcctgtac
     9421 ttccgctatt accatcacct cccccggcgt aaccggtttg cacgctatag ctgaggttgt
     9481 tgtcttccag caacgtaccg tatacaccga ccagattggt catccgaccg ttgagatcgt
     9541 gtgacatgct gtaactggca ctggcatgtc gccactgaga tttactgtca gaacgcagcc
     9601 agtggctgaa aggaatattg acgttaagcg ctaacatctg atcccgtcct ttttgccagg
     9661 cgtttttcgt caggctatag ctgagcgtcc agttgatatc ttcgaatgca gtatttaaac
     9721 cagcctggaa ttgctcatcg acattactcg ttccccaata agtttgatgg ctaccactca
     9781 aatacagtgt tgatgtgcgc ccgagttgct gagtaacggt gagttgtaat ttcccgcgtt
     9841 tgttataagc gagattgtaa tagtcggtga atttcggctt aacctgaata acaccatcct
     9901 gtgtttcgat gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc
     9961 cgctggtcga ataacggtaa cccactaact gaatattcgt gcctgattca ttgagcgatt
    10021 tgttatagag aaaacgcacc gattgtccgt catgctgact gtcatcggga agtgtggaat
    10081 tagcctgcgt catatccaca gacagagcgc ccagtgcccc catgtttttc ccgataccga
    10141 aattaaacgc acgataacga tccgccagtt gcgttccacc atatattgtc cagccagccg
    10201 gaaggccgtg gagtaatgta ctctggaaaa agcggggttt ttcctgctgc gcatttccac
    10261 tacggtattc tcctgccgta atggaataac gagtatgccc ttcacgttgc aaaagcggga
    10321 ctgacgaata gggtacggta aagatctgcg tgctgccgtc agcctcttta atcgttacct
    10381 gcaagtcacc actattacct gcggcataga tatcgttgat ggtaaaaggc cccggcggca
    10441 ccgtactatt ataaatgtca tacccatttt gtttaatagt gacctgtgca gtaccacgag
    10501 caataccgtg gatcaccggg gcaaatcctc tttggctatc gggtaacata ttgtcatctg
    10561 aggccaattg tgcgccgcga aagttaatac catcgaaaat atcgccctgg gtataaccat
    10621 cacccagcgt cagccgggaa cataacggaa ttatgtctcg ctcaagccag gtattgatat
    10681 gctgccattt atttttgcta cctgatgaac tgtcgctact gttataactc caggtggtat
    10741 tgtcgcgtaa acgccacgca ccaatattta acccactctg taggtttaaa tatgcataat
    10801 ggctgttacc cccaatccga ttctgtacac tatttccgct gaaattataa ttgagcaatc
    10861 cggcattaat accaggatcc cataactcag gaggaatata accacgcgcg cgattactca
    10921 taaatgcctg agggatcgtc aggttgagtc gctgctgacc aacatccaga tgcgcagtag
    10981 cgtcatggat cattgaggtt aatggcacgc aggcatcatc cgccagcaga ttcataccgg
    11041 agacagaagc cgtattcagc cccatacttg caagttgcgc gcgtgtcagg cagggaacaa
    11101 tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat
    11161 tcaaatagat atcgacacga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg
    11221 ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cggattaaaa tagagttcgg
    11281 cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aaagagccgg acaaaaaaac
    11341 cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat
    11401 tcagatatga cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag
    11461 gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat
    11521 aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc
    11581 tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg
    11641 ttaccgtcag gtaatagggt gtcgggttaa tcagagtcag agaattcgcg ctacgacgaa
    11701 atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg cgatagtaca
    11761 gtttaatgcg gctgataatt gcgagctgta gcgtattctc agtcattttg atttatccat
    11821 tgacggaatc gctttaacgt tcatccagaa taaactttcc cgatcctgtg gcaattggtt
    11881 atttgttgca tcaagaatac gtaaggtatt ctcttttttt cccttcatcg caaacagagg
    11941 tggcgtcacg ataaaacgac catcctttac accatcggca ttttccaccc atgattgaat
    12001 taaataggtg ctattttcat cattatttgt cacggcaagt tgcacttgtt tttgccctgc
    12061 cggataaatt acgcgagtcg cacctaaggc cactcccgct tcggcgcgtc cggcaaccat
    12121 cattgccatg aacatcagga tacctgccag caagcagaat gttatttcct gcgatttcct
    12181 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tgctgaacaa
    12241 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
    12301 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
    12361 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
    12421 gccaatattg gtggctatcc ctggcccctc tcccacggaa agcacatccg gatttttacc
    12481 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
    12541 taaatgaata acaaaaggca ccggtgcgct atcttcccct accgcatgaa accggttact
    12601 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
    12661 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtgt tccatttatt
    12721 tccggccaga gcaaacattg gcagcaacga cgctaataga aataatctct tccttttcat
    12781 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtccc tgaacctggg
    12841 taggttattg atactgaacc ttgaaggtcg catccgcatt agcagcaccc ggggttgcct
    12901 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
    12961 tttgtgcact aaatgtcgca ccgtccagcg tcagctcatt tcctgttctg tccagtatct
    13021 gcacaccaac atttgttgcg ctacccgcag ctgaactctg cagagccagt actttaggat
    13081 gagcactgtc aatcgcagtc cccaagaagg caacagcggc ttttgtggca acactggtat
    13141 cgcaatcatt cagctgaatg ttaaaaccga cagcagagct ggttgctcca gtctgcttca
    13201 gactagcggt acgaacctgg cccaactgaa cggtttgatc aacagagcct gcatcaactg
    13261 cgcaagcggc gttaacaact tcccctttaa agtgaacggt cccaccattt accgtcgtgg
    13321 gtgtagtttc agccagagcc gctgtagaac tcagggacag agccgacaga acaacaattg
    13381 ccagagtttt aattttcgta ctgctttcct ttcaaaaaac tatttctaaa tcgacatggg
    13441 cagtcgttcc gtacactttg ttttgtcaac gagtttgact gccaacactg cacagtttcc
    13501 ccccaaaaga tgaaacattt ggggccattt tgactcatag aggaaagcat cgcgcacaaa
    13561 ctttttcagt ttatttgttg gcttgatatt ctattgttat ctttatttat agatttttat
    13621 attgcatgag gtggtttttg gagagaagaa tgaggaagtt gcttcgagac acagaaacgt
    13681 tagctttaca tttagcggag gtgatgtgaa attaatttac aagagaaatg atttacatat
    13741 caaacagtta gatgcttttt gtcgttttat aatattttta tgcttgagaa aaaatacgta
    13801 acttatttat gatatggac
//
LOCUS       DBBGLQ010000118        13547 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.118, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000118 DBBGLQ010000000
VERSION     DBBGLQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.118"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            68..2296
                     /gene="crfC"
                     /locus_tag="ACIYSB_004428"
     CDS             68..2296
                     /gene="crfC"
                     /locus_tag="ACIYSB_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGC5431336.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVITHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2293..3171
                     /gene="rdcB"
                     /locus_tag="ACIYSB_004429"
     CDS             2293..3171
                     /gene="rdcB"
                     /locus_tag="ACIYSB_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGC5431337.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLLEKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGEHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            3435..4937
                     /gene="proP"
                     /locus_tag="ACIYSB_004430"
     CDS             3435..4937
                     /gene="proP"
                     /locus_tag="ACIYSB_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGC5431338.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5049..5138
                     /gene="pmrR"
                     /locus_tag="ACIYSB_004431"
     CDS             5049..5138
                     /gene="pmrR"
                     /locus_tag="ACIYSB_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGC5431339.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5114..6214)
                     /gene="pmrB"
                     /locus_tag="ACIYSB_004432"
     CDS             complement(5114..6214)
                     /gene="pmrB"
                     /locus_tag="ACIYSB_004432"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGC5431340.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(6215..6883)
                     /gene="basR"
                     /locus_tag="ACIYSB_004433"
     CDS             complement(6215..6883)
                     /gene="basR"
                     /locus_tag="ACIYSB_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGC5431341.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6880..8523)
                     /gene="eptA"
                     /locus_tag="ACIYSB_004434"
     CDS             complement(6880..8523)
                     /gene="eptA"
                     /locus_tag="ACIYSB_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGC5431342.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8627..9964)
                     /gene="adiC"
                     /locus_tag="ACIYSB_004435"
     CDS             complement(8627..9964)
                     /gene="adiC"
                     /locus_tag="ACIYSB_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGC5431343.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10101..10862)
                     /gene="adiY"
                     /locus_tag="ACIYSB_004436"
     CDS             complement(10101..10862)
                     /gene="adiY"
                     /locus_tag="ACIYSB_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGC5431344.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFVSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11187..13454)
                     /gene="adiA"
                     /locus_tag="ACIYSB_004437"
     CDS             complement(11187..13454)
                     /gene="adiA"
                     /locus_tag="ACIYSB_004437"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGC5431345.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3270 a         3318 c         3554 g         3405 t
ORIGIN      
        1 gaaaacagaa ctcaaaaaca atctggctaa ataaaataac aaaatttgct ttaaggaaga
       61 attttctatg tacacacaga ccctgtatga gttaagtcag gaggctgaac gcctgttaca
      121 gctttctcgc caacagttgc agttactgga aaaaatgcct ctctctgtac ccggagacga
      181 cgcgccacaa ctggctttac cctggagtca gcctaatatc gccgaacgtc acgcgatgct
      241 gaataatgag ttgcgtaaaa tttcccgact ggaaatggtg cttgcaattg tcggtaccat
      301 gaaagcaggg aaatcaacca ccattaatgc cattgttggt acggaggttc tgcctaatcg
      361 taatcgccca atgactgcgc tgccgacgct tattcgccat acgcccgggc aaaaggaacc
      421 ggtactgcat ttttcacatg tcgcgccaat cgattgttta attcaacaat tacaacagcg
      481 cctgcgtgat tgcgatatta agcatctgac cgatgtgctg gaaatagata aagatatgcg
      541 tgcgcttatg cagcggatcg aaaatggcgt cgctttcgaa aaatattatc tgggtgccca
      601 gcctattttt cattgtctga aaagtttgaa tgatttagtg cgactggcga aggcgctgga
      661 cgtcgatttt cctttttctg cttacgccgc cattgagcat attcccgtga ttgaagtgga
      721 gtttgtccat ctagcggggc tggagagtta tcccggtcag ttgacgttac tggatacccc
      781 cgggccaaat gaagccgggc aaccgcatct gcaaaaaatg cttaaccagc agctggcacg
      841 cgcctcggcg gtactggcgg tgctggatta tacgcaactg aaatcgatct ccgatgaaga
      901 ggtccgtgag gcgattttgg cggtggggca atcggtgccg ctgtacgtgc tggtcaataa
      961 gttcgatcaa caggatcgta acagtgacga cgccgaccag gtgcgggcac tgatttccgg
     1021 gacgctgatg aaaggctgta ttacgccaca gcagatattt ccggtgtcgt cgatgtgggg
     1081 ctacctggcg aatcgggcgc gctatgagtt agccaacaac ggtaagttac caccgccaga
     1141 gcaacaacgc tgggtggaag attttgccca tgccgcgctt ggcaggcgct ggcgtcatgc
     1201 cgatctggcg gacctcgaac atattcgtca tgctgccgat cagttgtggg aagattcgct
     1261 gttcgcccag ccaattcagg cgttgcttca tgccgcttac gctaacgcct cgttgtatgc
     1321 tctgcgatct gccgcgcata aactgttgaa ttacgcgcag caggcgcggg aatacctgga
     1381 ttttcgtgcg cacgggttaa acgtcgcttg tgaacaattg cggcaaaata tccaccagat
     1441 cgaagaaagt ttgcagctat tgcaactcaa tcaggcgcag gtgagcggcg agattaaaca
     1501 tgaaatcgag ctggccctga cctccgccaa ccactttctg cgtcaacagc aagatgcgct
     1561 gaaggtgcag ttagccgcct tgtttcagga tgattcggag ccgttaagcg agattcgtac
     1621 ccgctgtgag acactgttac agacggcgca gaacaccatc agtcgcgact ttacgctgcg
     1681 ttttgccgag cttgaatcca ccctttgccg ggtgttaacc gatgttattc gccccattga
     1741 gcaacaagtc aaaatggaat tgagcgagtc agggtttcgt cctgggtttc attttcctgt
     1801 ttttcacggc gtagttcccc acttcaacac tcgccagctg ttcagtgaag tcatttcgcg
     1861 ccaggaagca acggacgagc agagcacgcg tttaggcgtt gtgcgtgaga ctttttcgcg
     1921 ctggttgaat cagcccgact ggggacgggg aaatgagaaa tcaccgacag aaacggttga
     1981 ttacagtgtg ttgcaacgag cattaagcgc agaagtcgat ctctattgcc aacaaatggc
     2041 taaagttctg gcagagcagg tcgatgaatc tgttacggca ggcatgaata cttttttcgc
     2101 tgagttcgct tcatgtttga cggaattaca gacgcgttta cgcgaaagtc tggctctgcg
     2161 tcaacaaaat gaatcggtgg tcaggctgat gcagcagcaa ttgcagcaga ctgtgataac
     2221 tcacggctgg atttacaccg acgcccagct gttacgcgat gatattcaaa cacttttcac
     2281 ggcagaacga tattgaccaa gacgttactt gacggccccg gtcgcgtgct ggagtcggtt
     2341 tatccccgct ttttagtgga tctggcgcag ggtgatgatg cccgccttcc acaagcccat
     2401 cagcagcagt ttcgtgaacg actgatgcag gaacttcttt cgcgtgtgca gcttcagaca
     2461 tggacgaacg gcggcatgtt aaatgcgccg cttagcctgc gtctgacatt ggtggaaaaa
     2521 ctggcgtcga tgctggatcc cggtcatctg gcactgacgc agatcgcgca gcatctggcg
     2581 ctgctgcaaa aaatggatca ccgccagcac tctgctttcc cggagctccc ccagcaaatt
     2641 gccgccttgt atgagtggtt ttcagcccgt tgtcgctgga aggaaaaggc gttaacgcaa
     2701 cgaggcctac tggtgcaggc aggtgatcag agcgagcaaa tttttacccg ctggcgtgct
     2761 ggggcgtata acgcctggtc gttgcctggg cgctgtttta tcgttctgga ggagttgcgc
     2821 tggggggcat ttggcgatgc ctgccgtctg ggaagcccgc aagcggtggc gttgttgctg
     2881 ggtgatttgc tcgagaaagc gacacaacat ctggcagaga gtatcaatgc ggcaccgacc
     2941 acgcgtcact attaccatca gtggtttgcc tcttcgaccg ttccgacggg cggggagcat
     3001 gctgattttt taagttggct gggaaagtgg accacggcag ataaacaacc cgtttgctgg
     3061 tcagtgaccc aacgctggca aactgtcgcg ctggggatgc cacgactctg ttcagcgcag
     3121 cgtctggcgg gggcaatgct cgaggaaatc ttctctgtaa atttggcgta aataatcagt
     3181 tacatcaatg agtcctaaac gaaatccatg tgtgaagttg atcacaaatt taaacactgg
     3241 tagggtaaaa aggtcattaa ctgcccaatt caggcgtcaa ctggtttgat tgtacattcc
     3301 ttaaccggag ggtgtaagca aacccgctac gcttgttaca gagattgcat cctgcaattc
     3361 ccgctcccct tttgcggccg tcgcgctgat ttttctggcg tttgcggaaa tgggccaact
     3421 ctgcgaggaa agctatgctg aaaaggaaaa aagtaaaacc gattaccctt cgtgatgtca
     3481 ccattattga tgacggtaaa ctgcgtaaag ccattaccgc agcatcactg ggtaatgcaa
     3541 tggaatggtt cgattttggt gtttatggtt ttgttgctta cgcattaggt aaagtttttt
     3601 tcccgggggc tgaccccagc gtgcagatgg ttgctgcact tgccactttc tccgttccct
     3661 ttctgattcg accgcttggc ggactcttct ttggtatgtt gggcgataaa tatggtcgcc
     3721 agaagatcct cgctatcact attgtgatta tgtcgatcag tacgttctgt attggcttaa
     3781 taccgtccta cgacacgatt ggtatttggg caccgattct gctgttgatc tgtaagatgg
     3841 cacaaggttt ctcggtcggc ggtgaatata ccggggcgtc gatatttgtt gcggaatact
     3901 cccctgaccg taaacgtggc tttatgggca gctggctgga cttcggttct attgccgggt
     3961 ttgtgctggg tgcgggcgtg gtggtgttaa tttcgaccat tgtcggcgaa gcgaacttcc
     4021 tcgattgggg ctggcgtatt ccgttcttta tcgctctgcc gttagggatt atcgggcttt
     4081 acctgcgcca tgcgctggaa gagactccgg cgttccagca gcatgtcgat aaactggaac
     4141 agggcgaccg tgaaggtttg caggatggcc cgaaagtctc gtttaaagag attgccacta
     4201 aatactggcg cagcctgttg acatgtattg gtctggtaat tgccaccaac gtgacttact
     4261 acatgttgct gacctatatg ccgagttatt tgtcgcataa cctgcattac tccgaagacc
     4321 acggggtgct gattattatc gccattatga tcggtatgct gtttgtccag ccggtgatgg
     4381 gcttgctgag tgaccgtttt ggccgtcgtc cgtttgtgct acttggtagt gttgccctgt
     4441 ttgtgttggc gatcccggcg tttattctga ttaacagtaa cgtcatcggc ctgatttttg
     4501 ccgggttact gatgctggcg gtgatcctta actgctttac gggcgttatg gcttctacct
     4561 tgccagcgat gttcccgacg catatccgtt acagcgcgct ggcggcggca tttaatattt
     4621 cggtgctggt tgccggtctg acgccaacgc tggcggcctg gctggtcgaa agctcgcaga
     4681 atctgatgat gcctgcctat tacctgatgg tagtggcggt ggttggttta atcaccggcg
     4741 taaccatgaa agagacggca aatcgtccgt tgaaaggtgc gacaccggcg gcgtcagata
     4801 tacaggaagc gaaggaaatt ctcgtcgagc attacgataa tatcgagcag aaaatcgatg
     4861 atattgacca cgagattgcc gatttgcagg cgaaacgtac ccgcctggtg cagcaacatc
     4921 cgcgaattga tgaataagct gaaacggatg gcctgatgtg acgctgtctt atcaggccaa
     4981 ttgaactctt aaggttcact taatctctga cgcgcatact ctcctccagg ttaacggagg
     5041 agagtgcaat gaaaaaccgt gtttatgaaa gtttaactac cgtgttcagc gtgctggtgg
     5101 tcagcagctt tctttatatc tggtttgcca cgtactgatc tttcttcagc cgtacccagg
     5161 cccgcgtgcc ggaagtctct tgccggtttt gcaggaaaaa ctgcccgtga tgcaactgtg
     5221 taatgcggct gacaatactt aaccccagac caatcccgcc ataacggctg tccatacgta
     5281 caaacgcttt actcaactcc ccgcatttac tctcatcaat acctggtcct tcatcttcaa
     5341 ctgccatgac cgctccgtca tcttcttgca gcttaatcat aatgttgctg ccttgcgggc
     5401 tgtaacgatg ggcgttttct accaggtttc gcaataacat ccgcagcagg gttgcatcac
     5461 cctgaacggt gatgtcggcg gcgctctctg gcaatagcag ggtttgctgt cgctggtcga
     5521 gcatggtact gagttcgtca tacgagggga gaatgacatc ttccagcagt tttacatgtt
     5581 gataattacc ggaagaaaat gactgtccgg cacgcgccag ttgcagcagc tgggagacgc
     5641 tctccatcat ctgatcaagc cgtgccacta acggtgctac atcaatgtga tgcgttttcg
     5701 ccagcagttc cagatgcaaa cgcacccccg ccagtggcgt tcgcagttcg tgcgcgacgt
     5761 cagcggtaaa caacctttcg ttatccagcg tgctggtcag gcgactgacc agatcgttta
     5821 acgccgaaac caccgcttcg atttcgaggg tggcgctgtg aatggcaatg ggcgttaagt
     5881 tgtcggcggt gcgcgcttcc agctcttttt gcagctccgc cagcgggcgg gtgatgcggc
     5941 gtaccgcctg atagcagata aatagcgtca ggctgaccat aaagacgccg gggacaatca
     6001 ggctggcgac cgcctcgcgg atctcacgca tgatgtggcg atcgttgttg cgattgtcgc
     6061 gtagcgcctg ctcaaacagc tgaatctgct cggtactttc atgccatagc cagaagacgc
     6121 tgatcagctc aaacaccaac aaaatggccc cgatggtcaa tatcagccgt tggcgcagcg
     6181 atattggtcg gcgcagaaaa tgcatcagat tcaattagtt ttcctcattc gcgaccagca
     6241 tatagccaaa gccgcgcacg gtgcggatac gggctttgcc cactttgtcg cgcagattgt
     6301 ggatatgcac ttccagggtg ttggtcgagg gttcattgtc ccagttatag atgtcgttgt
     6361 agagaatttc ccgatgcacc ggactgcctg ctttgagcat taaccgtgac agcagagcat
     6421 attctttggg cgtcagaatc aactcttcac cgcccatcca tacctgacgg cgacccatgt
     6481 tcagcgtcag attgccaaca atcagctcac tttcgccctg attattatgg cgtcgtagca
     6541 gggcgcggat acgggcatgt aactcttcca gcgcaaaagg cttcaccaga tagtcgtcgg
     6601 caccgacatc cagcccggcg attttgtcgg tcagcgtatc gcgagcggtg aggatcagta
     6661 ccggcagggt atattttttc tgccggatac gggcgagaaa atgcagtcca tcttcgtcgg
     6721 gtaaccctaa atccagtacc accaggctgt aatgacctgc ctcaaggctt tgttccgcca
     6781 tccgcgcggt tgtcacgcca tcgcacgcgt agccttcggt ttgcgccgcc agaatcagtc
     6841 cctgcaataa cagcgtatcg tcttcaacaa tcagaatttt cattcactca ctctcctgca
     6901 agtttgcaga atatcatccg cagcctggta atacttcgtc tcaacgccag ttaatcccaa
     6961 tagcgtggag aataaattgt cttgtgaata gtgttgcgtt tgcgcctgtt tttgcaggca
     7021 gttctggtca acctgatacc gtttttgata atcctccgac agccacagca gcatcggcac
     7081 ctgtttttgg ctatccgggg cgatggcata aggcagaccg tgcagataga tgccattttc
     7141 acctaacgat tcaccgtggt cagaaagata aaccaggctg gtggtaaatt tatcctgatg
     7201 ttctttcagc agattaatcg ctttatcaac aatatagtcg acgtaaacca gcgtgttgtc
     7261 gtaagtgttc accagttgct ctttggtaca ggtctggatc tcattggtgt cgcaggttgg
     7321 ggtaaatttc ctgaactgag gcggatagcg gttgtaatag gtcggaccgt ggctgccgat
     7381 ggtgtgtaag acaatcacgc catcaccttg caggttattg atgtactctt caagcccgtg
     7441 gaacagcact tcgtcatagc attcgccgtt gatgcactga tcaggtagat tcagcgcggt
     7501 gacgttctgg tgaggcacgc ggtcgcaggc acctttacag ccgccatcgt tgtcattcca
     7561 cagcacgttg atgcccgctc gctgaatgat atccagcacg ccttcctggt gctgtgccag
     7621 ctcttctttg tagtgctcac gcggcatatc cgagaacatg cacggtactg aaactgccgt
     7681 tgccgtgccg caagatgcgg tattagggaa atagaccacg ttatctttcg ccagccgcgg
     7741 gttagtttca cgcgggtagc cgttgaggga gaagttctcc gcccgcgagg tttcgccgac
     7801 aatcaggatg gtcaaatttt tacgtttttc gttctgcatt aacgggttgc ggtgcgcgtc
     7861 ttcaccaatt cgcaccagcg gcagatttgc cagtcgctga tgggagtacc atgaccagct
     7921 ggcaacaatg ctgttagagg ggcttaagga tttcaccagc tctttgttat tgcggaacaa
     7981 cgaggcgtag tctttataaa acagtgcggc gaccagcaaa atcagtagta cagaaaccag
     8041 aatattggct ccacggaaaa gaacactgcg cagacgcgag gtggcaggtt tgatttttat
     8101 ccagcaggca atcagcgcag caagcacgcc gctgaatccc agcgttaata acatttgcgg
     8161 tgtcatcagc gcataacttt ctgccggagt ggtatcaata atattggcaa tcatcgagcg
     8221 gtcgatgacg atgccgtaag tcattatgaa atattgtgca gccgcgccaa ccagaataaa
     8281 caggcaggcc agtggtcgat taagccataa gaaagagctt agtgtcagga caatattaat
     8341 cacgctgaaa gcgacgaccg gcatcgacaa gaaaaccagt acgttatgca gcgaatccag
     8401 cggcagcgcc tgcaacacct gtttaaaaaa ggcaatattc aggcagatag agatataaaa
     8461 agcggccaac aatagccagg cgagtaaatt caaagagggt ctttttagta ggcgcttcaa
     8521 catcacggtg tttccatcga acaaagtgcg catatgctcg caaagcaaaa ttaagccaac
     8581 cttaagttct taaggttggc ttttatgttt gctggattaa ggggaattaa tctttgctta
     8641 ttggtgcatc taagggatac gggtttttat gtagccggtt gtaattcagc gcatacatgg
     8701 cggtgatgac catcagggtg acaaatgacc acataacctc tttcgctccg gaccccacca
     8761 cggcccagat gcagtagagg aaggcaatgg tagtaactgc cagatatgcc gggcgtgctt
     8821 taccaaagtg accgtgtccg agcagcagta acgccgcaca ggtgtaaaga tatggcacca
     8881 gtgtaaagat gaccgagacg gaagaaacca gaccgaactc tttggtcgcg tttggtgaaa
     8941 tgctgctgag ctggaagatg gtcatcaaaa taccgacgat aatcagcccc gccactggcg
     9001 tacccgcttt atttacacgg gcaaaaatcg gtgggaacag tccgtcatcg gcagcggctt
     9061 tcgccgtttg acccgccagc aacgtccagc cgcccagtga acctaagcaa cccgcagctg
     9121 cgcagaagga aacaatggcc ccggcggtgt cacccaacgc catccgtgcg gcatcaccga
     9181 atggcgaagc agaaacgcgc agtgcggcat tagggatcat ccccataatc gcggtggtag
     9241 aaagtacata gcaaacggcg gcaatcaata ccccaccaat ggtggcgata gggacattgc
     9301 gtttcgggtt tttcaccaca cctgcggcaa cggaggcact ttccacaccg atgaacgacc
     9361 acagcgtaac gttaagggta ctttgaattg caccgaaggt gcccaggccg ctgacgttcc
     9421 atgccgccat ataggtttca ccacggaacc agaaccagcc aaatacggca atcccgacga
     9481 tggggatcag cgccagcacg gtggcaactg cctgcacacg ggtgatcatt ttcggaccga
     9541 caatgttcag caggacgaag atccacagca ccacgacgca ggtgatggtt aataccaatg
     9601 gatctttcag aatcgggaag aagtaactta aatatcctac gccaatgacc accatggcga
     9661 tattgccgat ccagcaggcc agccagtaga ggacgttggt ttgataaccg agaaacgggc
     9721 caaagcagcg gcgggcgtaa gcgtaagaac caccaggact tgggtcgagg aacgacattt
     9781 tggcgtatac catcgagagc cccagtgcac cgataatcgt caccaaccat ccataaatgg
     9841 caatcccgcc agtagaggcc aggtttgcag gtaacagaaa aacacctgac cccataatat
     9901 tccccgacac catcagggtg acggggatta agcccacttt gtgagcatca gcatccgaag
     9961 acataattaa actcctgcga aggcgagctt cgtgacaata aattacgtca tatcatacgc
    10021 ctgcattcga tagttgaatt attatcgacc gggttatcgc tgatgccgtt attaatatag
    10081 ctcttcgtta aggttaaaaa tcaggcgaca gtgcgttctc tgtgctgact aacataatgc
    10141 agcggcgtca taccgtagaa gtctttaaag acagaaataa agtacgacgt actgttgtag
    10201 ccgcaggact gtgatacctg agagatattt ttaccgtcca tcattaattc atttacggca
    10261 taacgcatgc ggcaggtggt gattatttgg ctataactgg tgttttcgct tttcaacttt
    10321 ttctttaaca gacttgggct aagacataaa caactggcta ccatactaag attccagtct
    10381 ttgtgaatat cgctttcaat aatttgataa acgctgtcac ttacacaatt acgtaacata
    10441 tacatcatta atgaaacgaa ttttttactg tcgagaaaac gggatagcac cgtgaataat
    10501 aatgcgcgtg ttcgttcctt ttctgactca gtttctgcgg gcatcatgct gtgttgcgcg
    10561 gcaacgcgaa agacgtctgg cgtcaggcat ggcagggtga gtatgggcgt agcgctacgt
    10621 tgccatactg gtatttgcga gagatcttta ttcagaaacc ggaggtaatc tttgatgatg
    10681 tcgtgactaa tatgagcaac caaagtgttg ttaagagagg agacgtcgat aatattattt
    10741 tcacaattta ataacgccat atggttagct ttaaggctaa taggttcttt cccattcacc
    10801 cttatccaga catctttttc agtcaataaa acaatacaag gttggtcgct gcaaatcctc
    10861 atacatgtac tcctgagtgc gaataaaaga ccagttctcg ctaaagcaaa gcgataccgg
    10921 gaaatggtta tataattgta tgatgaatat aaacatgcat aaaaataacg agttttacta
    10981 atagtacaaa tttttatagt aatttctcat gtacatattt ttttacagga taaataacag
    11041 gcaaaaaaaa gcgcggggtg gggccgcgca aaaagtatga catttttgtt tgcagcaatt
    11101 tttatgaaaa cgttgccata ctcaaaaggc ctaaggccag gcggcgtaat gttatttaaa
    11161 caattacgcc ttcagcggaa tagtggttac gctttcacgc acataacgtg gtaaataccg
    11221 tcaataattt cagtcccttc agtttcgtgt tcaaatccag ggaaatggtg gtcccaggat
    11281 tgcagcgagc gtaaataact tacttgcgga ctgtttttat cgccgaagtt ttcaccagac
    11341 agcagcatcg ggattcctgg cggatacggg ataactgagt ttgccgcgat gcgtcctggc
    11401 agattttcaa tggataccag ttcgacattg ttgtcgacaa tcgcgttgta cgcttcacgc
    11461 ggggtgactt ccgccaccgg caggccggaa taggcttcgt tcaaccgtgc gccagggttg
    11521 ttttctttca gccaggcaaa catggtgtca cccagatcgt gaatccccat gttcgcgtaa
    11581 gtgtcaggat attgttcaac aagttccggc atcacctgcg ccagcggtgt gttggcgtca
    11641 tagtggcgtt tgaaggagca aagggtgtta accagagttc cccatttccc acgggttacg
    11701 cccatagaga acaggaacat aatttggaag tcagtggtgc gggtaggtac aatgccgtgg
    11761 cgaccaagcc aggcagtgac cagcgccgcc ggaacaccgg tttcttccag ttcaccatct
    11821 tcacccattc ccggagcaag gatgctgact ttaatcgggt cgagcatact ccagttatcc
    11881 ggaatatctt tgaagccgtg ccagctttcg cccggatgca ttacccagca gtcctgaacg
    11941 gtggtcagca gtttggttgg tgcgtcagca aagtcatagg ttttgccggt ttgtgggtcg
    12001 gtgacgactt ctttgttcca cggtttgaag aaccagctac cgtcagcggt gaactcttta
    12061 tatagccgcg ccatcgcctg acggaaatca accgcttcgt caatcacttc ctgtgtcagt
    12121 gacaggccgc tgttgccgtc catcatcgac accgccacgt cgttggatgc gcagatggca
    12181 tacagcgggg aggtggtggc atgcatcatg taggcctggt tgaagcggga gaagttaatc
    12241 gccccacgac cttcacgtac atgaatataa gaagcctgtg acagcgcatt cagcagtttg
    12301 tgggtggagt gggtggcgaa aacggtagga ccgttgtgat cgccaggttc gccgcgcatg
    12361 gcatagtgat cggcatagat cgggttgaaa cgtgcatagc cgtaccaggc ttcgtcaaag
    12421 tgcagacgat cggaggtttt ttccagcaga tcctgcgctt ctttagcgtt ataacacacg
    12481 ccgtcatagg tgcagttggt caccacgcag taagacggtt tttgcccggc tttgtctttg
    12541 gtcagcgggc tttcactgat tttcttctgc aaggtttcag gttgcatttc ctgcggatag
    12601 attggcccga taatgccgta gcggttgcgg cttggcacca tatagaccgg tttcgcgcct
    12661 gtcagcatca aaccttgttc gatggattta tggcagttac ggtcaacgac cacgacatcg
    12721 ttatcggtca tgcaagcctg catgatggtg cggttagagc cggaagtacc gacgactacc
    12781 gaccaggagc gatcggcacc aaatacgcgt gcggcatatt tttcgctttc gccaaatgcg
    12841 ccagtatggt caagcaaaga accgagggaa gttcgttcga tgcccatgtc ggtgcggaac
    12901 agattttcac catagtagtc atggtagaaa cgtccggcgg gtgttttggt aaaaccaacg
    12961 ccgccctggt ggcctggcgc tgcccaggaa tattcatgga tgtcactata tttcatcagc
    13021 gcgctgaaca gtggcggcaa cagctgctgg cggtagcggg tcatcgcggc aacggcgcgt
    13081 ccggcgataa agtcggcggt atcttccaga atccaggcga attcatcgac aagctccagc
    13141 aggtcgcgat ccattgcggc gagggctttt tcccgatcgc ccaacaggaa gaccggcacg
    13201 ttttgttggc gctcatgaag cttaccgatc aattgtctga cgttttgatg ttcgtccgga
    13261 tgttccattt gatagctgaa catcaggcag tcaatggctt cgtttgaaga gagaatggca
    13321 aaaccatcat caaaggaggt ggatttaatc acggtaacat tttgctggct taaagcatct
    13381 gccagacgct caacggcgtt accgacccag gtgtcttgat ggagaaactc gctttcaaca
    13441 attaatactt tcatcattgc ttacccggtt atgaaggaat cttcgttgcg ggcaagtatc
    13501 ttccggcctt ttcgggctgt aaagcgcgtg aaaaacaaac atttttg
//
LOCUS       DBBGLQ010000119        13319 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.119, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000119 DBBGLQ010000000
VERSION     DBBGLQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.119"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..401)
                     /gene="yhaJ"
                     /locus_tag="ACIYSB_004438"
     CDS             complement(<1..401)
                     /gene="yhaJ"
                     /locus_tag="ACIYSB_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGC5431346.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVL"
     gene            506..1207
                     /locus_tag="ACIYSB_004439"
     CDS             506..1207
                     /locus_tag="ACIYSB_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGC5431347.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1230..1394
                     /gene="yhaL"
                     /locus_tag="ACIYSB_004440"
     CDS             1230..1394
                     /gene="yhaL"
                     /locus_tag="ACIYSB_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGC5431348.1"
                     /translation="MSKKLAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1528..2838)
                     /gene="cyuA"
                     /locus_tag="ACIYSB_004441"
     CDS             complement(1528..2838)
                     /gene="cyuA"
                     /locus_tag="ACIYSB_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGC5431349.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2866..4197)
                     /locus_tag="ACIYSB_004442"
     CDS             complement(2866..4197)
                     /locus_tag="ACIYSB_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGC5431350.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4472..5836)
                     /gene="tdcG"
                     /locus_tag="ACIYSB_004443"
     CDS             complement(4472..5836)
                     /gene="tdcG"
                     /locus_tag="ACIYSB_004443"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGC5431351.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5908..6297)
                     /locus_tag="ACIYSB_004444"
     CDS             complement(5908..6297)
                     /locus_tag="ACIYSB_004444"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGC5431352.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6311..8605)
                     /gene="tdcE"
                     /locus_tag="ACIYSB_004445"
     CDS             complement(6311..8605)
                     /gene="tdcE"
                     /locus_tag="ACIYSB_004445"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGC5431353.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8639..9847)
                     /gene="tdcD"
                     /locus_tag="ACIYSB_004446"
     CDS             complement(8639..9847)
                     /gene="tdcD"
                     /locus_tag="ACIYSB_004446"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGC5431354.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRDSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGLEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9873..11204)
                     /gene="tdcC"
                     /locus_tag="ACIYSB_004447"
     CDS             complement(9873..11204)
                     /gene="tdcC"
                     /locus_tag="ACIYSB_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGC5431355.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11226..12215)
                     /gene="tdcB"
                     /locus_tag="ACIYSB_004448"
     CDS             complement(11226..12215)
                     /gene="tdcB"
                     /locus_tag="ACIYSB_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGC5431356.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12314..13252)
                     /gene="tdcA"
                     /locus_tag="ACIYSB_004449"
     CDS             complement(12314..13252)
                     /gene="tdcA"
                     /locus_tag="ACIYSB_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGC5431357.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3453 a         3577 c         3149 g         3140 t
ORIGIN      
        1 agcacttctg tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac
       61 gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa
      121 ccgcgcgcga gagcttccgc atcagtagtc agtttatctg cggcttccag caaaacgcgc
      181 cctcgctcca acaacatcct cccgacattg gtgaatttgg tacgatggcc cgagcggtca
      241 aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc
      301 acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat cgcatccata
      361 acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa
      421 tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa
      481 aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca
      541 agcagactac ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc
      601 gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc
      661 cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga
      721 agcagagtat cgcgatagcg aaggcaatca tgttcaggcc agcgccggag aggcgttgct
      781 gctctctacc cagccgggtg tcagctatag cgaacacaat ctcagcaaag acaaaccgtt
      841 aacgcgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa
      901 gctggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggtg cgatgggaag
      961 cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc
     1021 gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga aatttcatgc
     1081 gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga
     1141 cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc
     1201 tgtctagttg tttttacagg gagatgatga tgagtaaaaa attggccaaa aagcgccagc
     1261 cggtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag
     1321 agatgttgag cgagctggaa gctatcgtcg cggatgctga aacgcgttta gccgaggatg
     1381 aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa
     1441 atccaacaac attaacgaat taaacaaccc gtaggccgga caagatgcgc cagcatcgca
     1501 tccggcacga tccccaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat
     1561 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
     1621 ctcaacatca tgcgccacaa tcccttcatt gccggtcacg gcggtatcat ccagcgccat
     1681 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga
     1741 cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat
     1801 ggtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt
     1861 cgttgcggca cacagcgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa
     1921 atgcgaaagc atcagcgcac gcgccagccg ttcatcatcc gctccgaagt gttctgctac
     1981 caccaccaca ggcattgttg cggtaatccc ctggttaccc gagccggagt tactcatagc
     2041 cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga
     2101 tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat
     2161 atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga
     2221 atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc
     2281 cagcgtcgtt ctggaaagca ccgtcagcgg agactcttgc tcgccctctg ccacacacgc
     2341 ctgctgggta aacaccacac catcgtgcgt ctcgatatgc acaatgttgg tatgcccgcc
     2401 gacgatggtg acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat
     2461 ttcatcgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc
     2521 atcggcaatt gcctgcgctg ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc
     2581 acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt
     2641 gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg
     2701 accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca
     2761 tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt
     2821 taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag
     2881 gaacggagaa acacacagca acaaaccagt gacgataatc aggtacagag acatcccttt
     2941 gtatttgtgc aatgctggta ctttgtaaac cagccacgcc gggatcaggc accctaccat
     3001 gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc
     3061 gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacgagat tttcgttaat
     3121 cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg
     3181 aaagcctaaa tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct
     3241 gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc
     3301 ggaaatattc tgctcatagg cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga
     3361 cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa
     3421 tgctttatgc cgcgccactt caattgattt ttcccgcgag cgataagaga tcaccatcgg
     3481 acttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt
     3541 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
     3601 catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc cggtcgaaat
     3661 tttgaataac aatttctcgc cgcgtgagga gatcgccacc agaatgcaaa tcagtaccag
     3721 accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcaggtagga
     3781 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
     3841 atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacgctcgg
     3901 gtaatcttta cattctggtg attctgccag cgtattaata aacaatcgct gaaacagata
     3961 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
     4021 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
     4081 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
     4141 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat
     4201 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
     4261 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
     4321 aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt
     4381 aatcgcagga gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
     4441 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
     4501 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
     4561 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
     4621 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
     4681 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
     4741 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
     4801 ccacgccaat ctccccctga cagccgactt ccgcgccaga gatggaggcg ttcattttat
     4861 acagcgcgcc aatagccccc gcggccagaa aatagcgggc aattgaccgc tcgtttaccg
     4921 gacgacggaa cttatcgtaa taagccagta ctgccggaat aatgccgcac gcaccgttag
     4981 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
     5041 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgctg gaaaccagct
     5101 gacgacgcag cgctacggca cggcgcggca cattgagcgg accaggcagc acgccttcag
     5161 tgttcatccc acgttcaata ccgtcatgca tcacttgcca gatacgggca aaaccggcgt
     5221 caatttccgc tttgctgcgc agcgctagct cgttgtgcat catcagacca gatatagaca
     5281 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
     5341 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
     5401 cagagtaata ggttttactt aatagctctt cctgtccctt ccaggcagtg atccgcattc
     5461 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
     5521 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
     5581 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgatga
     5641 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac agatcgacca
     5701 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
     5761 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
     5821 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
     5881 ccttacgacc gtcccgctca cgaggcttta cgcactacgt actgcgatgg cttcaatttc
     5941 cagcttcaca tctttcggca aacgcgcgac ctgcacacag ctccgggtcg gataggtcgc
     6001 ctgatgctca tcgaagaact gcttatagac ttcgttgatg gtggcaaaat cattcagatc
     6061 ggtgataaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
     6121 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
     6181 ggtctgtggg caaaccggta tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
     6241 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
     6301 tcctccggcg tcagagcgcc tgggtaaagg tacgtgaaat aacatcctgt tgctgttcac
     6361 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
     6421 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
     6481 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
     6541 tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
     6601 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
     6661 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
     6721 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
     6781 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
     6841 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
     6901 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
     6961 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
     7021 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
     7081 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
     7141 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
     7201 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
     7261 agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
     7321 ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca
     7381 gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
     7441 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
     7501 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
     7561 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
     7621 caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca
     7681 gttcctgtgc ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca atgtagatat
     7741 cgaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat ttcactgccg
     7801 ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga
     7861 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
     7921 ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact
     7981 ggagatcggc aaattgcagt tcgcgttcac gtaccagata actgatgcca tacagcgcta
     8041 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
     8101 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
     8161 gggttttacg cagatcggta aacagatatt caaattcact gtccatttct cggccatagg
     8221 cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg
     8281 gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat
     8341 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcatttt
     8401 cgatacggat gccttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg
     8461 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
     8521 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
     8581 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
     8641 atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
     8701 tttcttcgtt agtcggaata acggcacaaa tgacacgcgc attttcactg gaaacaattc
     8761 gctcaccaca ggagttagag cgattattca tttctgtatc aatctctaag cctaatacag
     8821 ccaaatgttc catgaccaga cgacgaatta agcttgaatt ctctcctatt ccgccggtga
     8881 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
     8941 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
     9001 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
     9061 ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac
     9121 caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
     9181 ttgaggtatc aacactctga ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat
     9241 gcgccacaac caggccggag tcatcttccg ccagattcag cagcgaatgt gcgcgctggg
     9301 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
     9361 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
     9421 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
     9481 aattggcgta attatgcagg ggtgccagtg gagaatcgcg acggatatta tcaatgactt
     9541 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
     9601 ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
     9661 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
     9721 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
     9781 cgagcacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
     9841 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
     9901 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
     9961 agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
    10021 ggtgcgccca tggcttcaat caggtcaagg atgttcgggt tggcgtaggc aacaacccag
    10081 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt
    10141 ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag cgttcccaga
    10201 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    10261 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
    10321 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagagtaaac
    10381 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    10441 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgc
    10501 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
    10561 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    10621 tcaacctggt cgataactgc agagttccag taagggatca gcgacaaaga aatcagcacc
    10681 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    10741 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
    10801 ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    10861 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    10921 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    10981 agacacagac gcgccagcgc ccggtggcaa taaaacgcga tggggtatgc caataccaac
    11041 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    11101 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    11161 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    11221 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    11281 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
    11341 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    11401 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    11461 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    11521 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    11581 ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
    11641 ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga
    11701 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
    11761 ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
    11821 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
    11881 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
    11941 agggaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
    12001 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
    12061 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
    12121 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatgtca
    12181 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
    12241 atatgtaggt aaccataacc gacacctgca agacagacag gtggattatt tatgtttaaa
    12301 taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca ttataagatg
    12361 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    12421 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    12481 gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
    12541 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    12601 catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca
    12661 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    12721 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    12781 tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
    12841 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    12901 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    12961 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    13021 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
    13081 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
    13141 cggccggttg agttaaccct aattcttttg cagccgagcc gatagaacca cttctaatga
    13201 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    13261 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtg
//
LOCUS       DBBGLQ010000120        12930 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.120, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000120 DBBGLQ010000000
VERSION     DBBGLQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.120"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(159..1310)
                     /gene="argE"
                     /locus_tag="ACIYSB_004450"
     CDS             complement(159..1310)
                     /gene="argE"
                     /locus_tag="ACIYSB_004450"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGC5431358.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            1464..2468
                     /gene="argC"
                     /locus_tag="ACIYSB_004451"
     CDS             1464..2468
                     /gene="argC"
                     /locus_tag="ACIYSB_004451"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGC5431359.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYKKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            2476..3252
                     /gene="argB"
                     /locus_tag="ACIYSB_004452"
     CDS             2476..3252
                     /gene="argB"
                     /locus_tag="ACIYSB_004452"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGC5431360.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            3313..4686
                     /gene="argH"
                     /locus_tag="ACIYSB_004453"
     CDS             3313..4686
                     /gene="argH"
                     /locus_tag="ACIYSB_004453"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGC5431361.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            4953..5870
                     /gene="oxyR"
                     /locus_tag="ACIYSB_004454"
     CDS             4953..5870
                     /gene="oxyR"
                     /locus_tag="ACIYSB_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGC5431362.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(5853..7253)
                     /gene="sthA"
                     /locus_tag="ACIYSB_004455"
     CDS             complement(5853..7253)
                     /gene="sthA"
                     /locus_tag="ACIYSB_004455"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGC5431363.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            7584..8234
                     /gene="fabR"
                     /locus_tag="ACIYSB_004456"
     CDS             7584..8234
                     /gene="fabR"
                     /locus_tag="ACIYSB_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGC5431364.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            8234..8593
                     /gene="yijD"
                     /locus_tag="ACIYSB_004457"
     CDS             8234..8593
                     /gene="yijD"
                     /locus_tag="ACIYSB_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGC5431365.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(8633..9733)
                     /gene="trmA"
                     /locus_tag="ACIYSB_004458"
     CDS             complement(8633..9733)
                     /gene="trmA"
                     /locus_tag="ACIYSB_004458"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGC5431366.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      9847..10037
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10102..11946
                     /gene="btuB"
                     /locus_tag="ACIYSB_004459"
     CDS             10102..11946
                     /gene="btuB"
                     /locus_tag="ACIYSB_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGC5431367.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            11891..12748
                     /gene="murI"
                     /locus_tag="ACIYSB_004460"
     CDS             11891..12748
                     /gene="murI"
                     /locus_tag="ACIYSB_004460"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGC5431368.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPGAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         3011 a         3174 c         3551 g         3194 t
ORIGIN      
        1 cggatgcggc gcgagcgcct tatccggcct acggttgggc atcgtttgag tcactgtagg
       61 tcggataaga tgcgcaagca tcgcatccga cattattgcg gcactggagt ttggcaacag
      121 cgccggatgc ggcgcgagcg ccttatccgg cctacgtttt aatgccagca aaaatggtga
      181 attacctggg ttatcagttc gcgggtgggc ttgataaacc gtgtttccag atattcatca
      241 ggttgatgag cctgattaat tgagccaggc cccaacacca gcgtcgggca taacgtttga
      301 ataaacggcg cttcggtaca gtagttcacc acttcggttt ttgctccgag caatttctca
      361 accacttcaa ccagttgatg attcggtggg cattcatagc cagggatcgg cggatgcagc
      421 tcgtcgaccg tcagacgacc cggccagcgt tcgctcaccg gagccaatgc atcgttgagc
      481 aaaccattaa gttcattgag tgtcatgcca ggcagcggac gaatatccat atgcaactca
      541 cagcaagcgc aaatacggtt agaagcgtcg ccaccgtgaa tatgcccgag gttgagcgta
      601 gggtatggca cggtaaacgc ttcgtagtga taacgttctt tcaggttatc gcgcaattgc
      661 aaaatatgcc cgatggcgtc gtgcattagt tcgatagcgt taactccgcg tgctggatcg
      721 ctggagtgcc ccgactggcc ctgaatacgg atggcgttag agatatgacc tttatgtgcg
      781 cgtaccggtt gtagtgacgt cggttcgcca atgatggcgc aatccgggcg cagggcggta
      841 gtttcggcaa aataacgcgc tccggccata ctggtttctt catcagcagt cgccagaatg
      901 tagagcggtt ttttcagttt cgtgacgtcg acatcgcgta gcgcatcaag gataaacgca
      961 aaaaagcctt tcatgtcggc ggtgcctaag ccgtaaagct tgccgtcatg ctccgtcagt
     1021 gtaaacggat cgcgcgtcca gcgaccgtca tcaaatggca ccgtatcggt atgccccgcc
     1081 agcaacaagc cgccagcccc ctgtccgata cttgccagca tattgaattt gttgcgagtt
     1141 cctggaacag gctgcacttc cacattgaag cccaaatctt taaaccagtc cgccagcaga
     1201 gtgattaaat ctgcattgct ttgatcgagt gcctcttccg tggcgcttat tgaaggtgtg
     1261 gcaatcagag cgcggtaaat ctcgataaat ggcggtaatt tgtttttcat tgttgacaca
     1321 cctctggtca tgatagtatc aatattcatg cagtatttat gaataaaaat acactaacgt
     1381 tgagcgtaat aaaacccacc agccgtaagg tgaatgtttt acgtttaacc tggcaaccag
     1441 acataagaag gtgaatagcc ccgatgttga atacgctgat tgtgggtgcc agcggctacg
     1501 ctggcgcaga gctagtgacc tatgtaaatc gccatccgca tatgaacata accgctttga
     1561 ctgtttcagc gcaaagcaat gatgcgggaa agttaatctc cgatttgcat ccgcagctaa
     1621 aaggcatcgt tgatctgccg ttgcagccga tgtcggatat cagcgagttt agcccagggg
     1681 tggacgtagt gtttctcgcc accgcccatg aagttagcca cgatttagcg ccgcagtttc
     1741 ttgaagcggg ctgcgtggtg ttcgaccttt ccggcgcgtt tcgtgttaac gacgccacct
     1801 tctataaaaa atattacggc tttacccatc aatacccgga actgttggaa caggcagcct
     1861 acggtctggc ggagtggtgc ggtaataaat taaaagaagc gaatttgatt gcggtgccgg
     1921 gctgttatcc gacggcggca cagctggcgc tgaaaccgtt gattgatgcc gatcttcttg
     1981 acctcaatca gtggccggtg atcaacgcca ccagcggcgt gagcggtgca gggcgtaaag
     2041 cggccatttc aaacagcttt tgtgaagtta gcctgcaacc gtatggcgtc tttactcatc
     2101 gccatcaacc agagatcgcc acacacctcg gtgctgacgt tatcttcacc ccacatctgg
     2161 gcaatttccc gcgcggcatt ctcgaaacca ttacctgccg cctgaaatcg ggtgtgaccc
     2221 aggcgcaagt cgcgcaagtg ttacagcagg cgtatgccca taaaccgctg gtgcggctgt
     2281 atgacaaagg cgttccggcg ctgaaaaatg tcgttgggct gccattttgc gatatcgggt
     2341 ttgccgttca gggcgagcat ctgattattg tggcgaccga agacaactta ctgaaaggcg
     2401 cggcggcaca agcggtacag tgcgccaata ttcgtttcgg ctatgcggaa acgcagtctc
     2461 ttatttaagg gtgcaatgat gaatccatta attatcaaac tgggcggcgt actgctggat
     2521 agtgaagagg cgctggaacg tctgtttagc gcactggtga attatcgtga gtcacatcag
     2581 cgtccgctgg tgattgtgca cggcggcggt tgcgtggtgg atgagctgat gaaagggctg
     2641 aatctgccgg tgaaaaagaa aaacggcctg cgggtgacgc ctgctgatca gatagacatt
     2701 atcaccggag cactggcggg aacggcaaat aaaaccctgt tggcatgggc gaagaaacat
     2761 cagattgcgg ccgtaggttt gtttctcggt gacggcgaca gcgtcaaagt gacccagctt
     2821 gatgaagagt taggtcatgt tggactggcg cagccaggtt cgcctaagct tatcaactcc
     2881 ttgctggaga acggttatct gccggtggtc agctccattg gcgtaacaga cgaagggcaa
     2941 ctgatgaacg tcaatgccga ccaggcggca acggcgctgg cggcaacgct gggcgcggat
     3001 ctgattttgc tctccgacgt cagcggcatt ctcgacggca aagggcaacg cattgccgaa
     3061 atgaccgccg cgaaagcaga acaactgatt gagcagggca ttattactga cggcatgata
     3121 gtgaaagtga acgcggcgct ggatgcggcc cgcacgctgg gccgtccggt agatatcgcc
     3181 tcctggcgtc atgcggagca gcttccggca ctgtttaacg gtatgccgat gggtacgcgg
     3241 attttagctt aagttttgtt ggccggaggc gcagctttcc ggcattgaat ttcaaaataa
     3301 ggaaacagag ttatggcact ttggggcggg cgttttaccc aggcagcaga tcaacggttc
     3361 aaacaattca acgactcact gcgctttgat taccgtctgg cggagcagga tattgttggc
     3421 tctgtggcct ggtccaaagc cctggtcacg gtaggcgtgt taaccgcaga agagcaggcg
     3481 caactggaag aggcgctgaa cgtgttgctg gaagatgttc gcgccaggcc acaacaaatc
     3541 cttgaaagcg acgccgaaga tatccatagc tgggtggaag gcaaactgat cgacaaagtg
     3601 ggccagttag gcaaaaagct gcataccggg cgtagccgta atgatcaggt agcgactgac
     3661 ctgaaactgt ggtgcaaaga taccgttagc gagttactga cggctaaccg gcagctgcaa
     3721 tcggcgctgg tggaaaccgc acaaaacaat caggacgcgg taatgccagg ttacactcac
     3781 ctgcaacgcg cccagccggt gacgttcgcg cactggtgcc tggcctatgt tgagatgctg
     3841 gcgcgtgatg aaagccgttt gcaggatgcg cttaagcgtc tggatgtcag cccgctaggc
     3901 tgtggcgcgc tggcgggaac ggcctatgaa atcgaccgtg aacagttagc aggctggctg
     3961 ggctttgctt cggcgacccg taacagtctc gacagcgttt ctgaccgtga ccatgtgttg
     4021 gaactgcttt ctgctgccgc tatcggcatg gtgcatctgt cgcgttttgc tgaagatctg
     4081 attttcttta acaccggcga agcggggttt gtggagcttt ctgaccgcgt gacttccggt
     4141 tcatcattaa tgccgcagaa gaaaaacccg gatgcgctgg agctgattcg cggtaaatgc
     4201 ggccgggtgc agggggcgtt aaccggcatg atgatgacgc tgaaaggttt gccgctggct
     4261 tacaacaaag atatgcagga agacaaagaa ggtctgttcg acgcgctcga tacctggctg
     4321 gactgcctgc atatggcggc gctggtgctg gacggcattc aggtgaaacg tccacgttgc
     4381 caggaagcgg ctcagcaggg ttacgccaac gccaccgaac tggcggatta tctggtggcg
     4441 aaaggcgtac cgttccgcga ggcgcaccat attgttggtg aagcggtggt ggaagccatt
     4501 cgtcagggca aaccgctgga agatctgccg ctcagtgagt tgcagaaatt cagtcaggtg
     4561 attgacgaag atgtctatcc gattctgtcg ctgcaatcgt gcctcgacaa gcgtgcggca
     4621 aaaggcggcg tctcaccgca gcaggtggcg caggcgattg cttttgcgca ggctcggtta
     4681 gggtaagaac atttatatgt ataaatttga gcctggctta tcgccgggct tttttatggc
     4741 aaaaaaaagc ggatcctgga gatccgcaaa agttcacgtt ggctttagtt attcgagttg
     4801 agaaactctc gaaacgggca gtgacttcaa gggttaaaag aggtgccgct ccgtttctgt
     4861 gagcaattat cagtcagaat gcttgatagg gataatcgtt cattgctatt ctacctatcg
     4921 ccatgaacta tcgtggcgat ggaggatgga taatgaatat tcgtgatctt gagtacctgg
     4981 tggcattggc tgaacaccgc cattttcggc gtgcggcaga ttcctgccac gttagccagc
     5041 cgacgcttag cgggcaaatt cgtaagctgg aagatgagct gggcgtgatg ttgctggagc
     5101 ggaccagccg taaagtgttg ttcacccagg cgggaatgct gctggtggat caggcgcgta
     5161 ccgtgctgcg tgaggtgaaa gtccttaaag agatggcaag ccagcagggc gagacgatgt
     5221 ccggaccgct gcacattggt ttgattccca cagttggacc gtacctgcta ccgcatatta
     5281 tccctatgct gcaccagacc tttccaaagc tggaaatgta tctgcatgaa gcacagaccc
     5341 accagttact ggcgcaactg gacagcggca aactcgattg cgtgatcctc gcgctggtga
     5401 aagagagcga agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct
     5461 atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg gcaggggaaa
     5521 aactgctgat gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt ttctgttttg
     5581 aagccggggc ggatgaagat acacacttcc gcgcgaccag cctggaaact ctgcgcaaca
     5641 tggtggcggc aggtagcggg atcactttac tgccagcgct ggctgtgccg ccggagcgca
     5701 aacgcgatgg ggttgtttat ctgccgtgca ttaagccgga accacgccgc actattggcc
     5761 tggtttatcg tcctggctca ccgctgcgca gccgctatga gcagctggca gaggccatcc
     5821 gcgcaagaat ggatggccat ttcgataaag ttttaaaaca ggcggtttaa accgtttaac
     5881 gcagctaccc gataggcttc cgccatcgtc gggtagttaa aggtggtgtt gacgaagtac
     5941 tcaatagtgt tgccgccacc tttctgttcc ataatcgcct gaccgatatg aataatttcg
     6001 gcagcgcgct cgccaaagca gtgaataccc agaatctctt ttgtttcccg atggaacaaa
     6061 attttcagcg tgcccacgtt catgccgacg atttgtgcgc gtgccagatg tttaaactgg
     6121 gcgcggccca cttcatatgg cactttcatt gcggtcagct gctgttcggt tttgcccaca
     6181 gagctgattt ccgggatggt gtaaataccg gtagggatat cttcaatcag atgtgcggtg
     6241 gcttcgcctt ttaccagcgc ctgcgcggca atgcgcccct ggtcataggc cgccgacgcc
     6301 aggctcggat aaccaatcac gtcgcccacc gcgtaaacgt gtggctgtgc ggtctgatac
     6361 atgctgttga ccttcagctg tccgcggctg tcagtttcta gcccaatgtt ctgtaacgcc
     6421 agcgaatcgg tattaccggt gcgaccgttg gcatagagca ggcagtcagc tttcagtttt
     6481 ttacccgact tcagatgcat gatcacacca tcgtcacagc cttcgatctt ctcgtactct
     6541 tcgttgtgac gaatcactac gccactgttc cagaagtgat aggagagaga atctgacatc
     6601 tcttgatcga gaaatgccag caggcgatcg cgggtgttga tcagatccac ttttacatcc
     6661 ataccgcgga agatcgacgc atattcacag ccgatcactc cagcaccata gataagtaca
     6721 tggcgcggtt cgtggtgcat gctgagaatt gagtcgctgt cgtaaatgcg tggatgggtg
     6781 aaatcaacat ctgttggatg atatggacga gagccgcagg caataacaaa tttttcagcg
     6841 gttagtgttt caacgctgcc gtccgggcaa tccagcgcca acgtatgctc gtcaacaaag
     6901 cgagcgtttc cctgcaatat ttcacagtga ttacgttcgt aaaatccctg acgcatgcgc
     6961 gtttgttgat taatcacgtt atcggcatgg ttaaggatat cggcaaaaga agagcggagc
     7021 agtcgggaat ggtcgctgta aagtgggttt tgattgaatt ctataatgcg gctgacggcg
     7081 tgacggagag ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc aacattttga
     7141 taacgctcga taactgcgac gcgcgcacct tgcttaacca ggcccattgc agcgccttcg
     7201 ccgccggggc cggaacctat tactatggca tcgtaatcgt aggaatgtgg catggtaggg
     7261 cttacctgtt cttatacata aaagcaacag aatggtaaca ttttatcgcg ggtaagccaa
     7321 ttgatccccg tcatttatct ggctatatcc tgagcggcct ttgctttgtc tgtttcttac
     7381 ttttgccctg acgttttatt ggatttttat cgacgatact ctccgtttaa gcggcaggtt
     7441 tccgctgtac gtaaaagaac cggccaaaga attgcagtaa atatgtttta ttgcgttacc
     7501 gttcattcac aatactggag caatccagta tgttcattct ctggtatagt gccagcagta
     7561 cttttggcaa ggattcagac atcgtgatgg gcgtaagagc gcaacaaaaa gaaaaaaccc
     7621 gccgttcgct ggtggaagcc gcatttagcc aattaagtgc tgaacgcagc ttcgccagcc
     7681 tgagtttgcg tgaagtggcg cgtgaagcgg gcattgctcc cacctctttt tatcggcatt
     7741 tccgcgacgt agacgaactg ggtctgacca tggttgatga gagcggttta atgctacgcc
     7801 aactcatgcg ccaggcgcgt cagcgtatcg ccaaaggcgg gagtgtgatc cgcacctcgg
     7861 tctccacatt tatggagttc atcggtaata atcctaacgc cttccggtta ttattgcggg
     7921 aacgctccgg cacctccgct gcgtttcgtg ccgccgttgc gcgtgaaatt cagcacttca
     7981 ttgcggaact tgcggactat ctggaactcg aaaaccatat gccgcgtgcg tttactgaag
     8041 cgcaagccga agcaatggtg acaattgtct tcagtgcggg tgccgaggcg ttggacgtcg
     8101 gcgtcgaaca acgtcggcaa ttagaagagc gactggtact gcaactgcga atgatttcga
     8161 aaggggctta ttactggtat cgccgtgaac aagagaaaac cgcaattatt ccgggaaatg
     8221 tgaaggacga gtaatgaaac aagcaaatca agatagaggt acgctgctgc tggcgttagt
     8281 tgctggctta tcgattaatg gtactttcgc agcgctgttt agctccattg tgccattttc
     8341 tgtattcccg attatttccc tggtgctgac ggtttactgc ctgcatcaac gttatcttaa
     8401 tcgcactatg ccggtaggct tgccgggtct ggcagctgcc tgttttattc tcggcgtact
     8461 gctgtacagc acggtagttc gtgcggaata tccggatatc ggctctaact tcttcccggc
     8521 agtactctcc gtcattatgg tgttctggat tggcgcgaag atgcgtaacc gtaagcagga
     8581 agttgctgag taatcggtat tatgccggat gctggcgcat ccggcatggg ttttacttcg
     8641 cggtcagtaa tacgccgcac tccatatggt gcgtgtaggg gaactgatca aacagagcca
     8701 gacgttcgac cttgtgcgtc tggcttaatg tttccagatt cttgcataac gtttccgggt
     8761 tacaggagat gtacaaaata cgcggatacg cctgcaccat tttctcggtt tcactgtcca
     8821 gaccgctgcg cggtgggtcg acaaaaatgg tttcgcactg ataactcttt aagtcgatcc
     8881 cttgcaggcg gttaaactcg cgcacaccat tcatcgcctg agtaaattct tctgccgcca
     8941 tacgaataat ttgtacgtta tcaatatggt tagctgcgat gttgtattgc gcagcagcaa
     9001 ccgacggctt agcgatttcg gtggctaata cccgatcaaa attacgcgcc agcgctaatg
     9061 aaaagttacc gttgccgcag tacagctcca gtaaatcgcc ttttgagcct ttggttacgt
     9121 ccagcgccca ttccagcatc tgaatattca tcgccgcgtt cggctgggta aagctgtttt
     9181 ctacctgacg gtagatcatc tctttccctg cgaccggcag acgttcatcg atgtaatcct
     9241 gatccagctc gattttggtt ttcgttgccc gaccaatcag atgcacattc agattctgcg
     9301 cgcgcagtgc atcgcgcagg gcctccgctt cctgacgcca ctcatcatcc agcttcttat
     9361 ggtatagcag ggaaaccacc gcctgattac tcagcgtagt gaggtaatca atctggaaca
     9421 acttgtggcg cagaacggga ttattacgca cccccgcaat catcgccgtc atcaactggt
     9481 tgataagttc actggcggcg gggaagctat ccacgcggat gcggcttttg gtttgttgat
     9541 cgaaaatgat gtgatacagg tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc
     9601 ggtaatgact gaccggcgag cgaaacactt ccggaaccag gtcagaaaac ggtgccatca
     9661 tactttgcaa acgtaccact ttttcggcta actgcgcttc atactgttct gttggaaggt
     9721 gttcgggggt catgatgtat cctgaaaaat taaagtacgc ggcgattgta gggattgctc
     9781 atcagatgtc cagatcttga tgaattccta tttgtgagct acgtctggac agtaacttgt
     9841 tacaacctgt agcatccact tgccggtcct gtgagttaat agggaatcca gtgcgaatct
     9901 ggagctgacg cgcagcggta aggaaaggtg cgatgattgc gttatgcgga cactgccatt
     9961 cggtgggaag tcatcatctc ttagtatctt agatacccct ccaagcccga agacctgccg
    10021 gccaacgtcg catctggttc tcatcatcgc gtaatattga tgaaacctgc ggcatccttc
    10081 ttctattgtg gatgctttac aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc
    10141 acggcatttt ccgcttgggc acaggatacc agcccggata ctctcgtcgt tactgctaac
    10201 cgttttgaac agccgcgcag cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat
    10261 atcgaccgct ggcagtcgac ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat
    10321 atcacccaaa acggcggttc aggtcagctc tcatctattt ttattcgcgg tacaaatgcc
    10381 agtcatgtgt tggtgttaat tgatggcgta cgcctgaatc tggcgggggt gagtggttct
    10441 gccgacctta gccagttccc tattgcgctt gtccagcgtg ttgaatatat ccgtgggccg
    10501 cgctccgctg tttatggttc cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc
    10561 gatgaacccg gaacggaaat ttcagcaggg tggggaagca atagttatca gaactatgat
    10621 gtctctacgc agcaacaact gggggataag acacgggtaa cgctgttggg cgattatgcc
    10681 catactcatg gttatgatgt tgttgcctat ggtaataccg gaacgcaagc gcagacagat
    10741 aacgatggtt ttttaagtaa aacgctttat ggcgcgctgg agcataactt tactgatgcc
    10801 tggagcggct ttgtgcgcgg ctatggctat gataaccgta ccaattatga cgcgtattat
    10861 tctcccggtt caccgttgct cgatacccgt aaactctata gccaaagttg ggacgccggg
    10921 ctgcgctata acggcgaact gattaaatca caactcatta ccagctatag ccatagcaaa
    10981 gattacaact acgatcccca ttatggtcgt tatgattcgt cggcgacgct cgatgagatg
    11041 aagcaataca ccgtccagtg ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg
    11101 ggtgtcgact ggcagaaaca gactacgacg ccgggtacag gttatgttga ggatggatat
    11161 gatcaacgta ataccggcat ctatctgacc gggctgcaac aagtcggcga ttttaccttt
    11221 gaaggcgcag cacgcagtga cgataactca cagtttggtc gtcatggaac ctggcaaacc
    11281 agcgccggtt gggaattcat cgaaggttat cgcttcattg cttcctacgg gacatcttat
    11341 aaggcaccaa atctggggca actgtatggc ttctacggaa atccgaatct ggacccggag
    11401 aaaagcaaac agtgggaagg cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt
    11461 tccggatatc gtaacgatgt cagtgacttg atcgattatg atgatcacac cctgaaatat
    11521 tacaacgaag ggaaagcgcg gattaagggc gtcgaggcga ccgccaattt tgataccgga
    11581 ccactgacgc atactgtgag ttatgattat gtcgatgcgc gcaatgcgat taccgacacg
    11641 ccgttgttac gccgtgctaa acagcaggtg aaataccagc tcgactggca gttgtatgac
    11701 ttcgactggg gtattactta tcagtattta ggcactcgct atgataagga ttactcatct
    11761 tatccttatc aaaccgttaa aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat
    11821 ccggtcacct ctcacctgac agttcgtggt aaaatagcca acctgttcga caaagattat
    11881 gagacagtct atggctacca aactgcagga cgggaataca ccttgtctgg cagctacacc
    11941 ttctgaacca cgtcccaccg tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta
    12001 tgacgagatc cggcatctct taccggatct ccattacatt tatgctttcg ataacgtcgc
    12061 tttcccgtat ggcgaaaaaa gcgaagcgtt tattgttgag cgagtggtgg caattgtcac
    12121 cgcggtgcaa gaacgttatc cccttgcgct ggctgtggtc gcttgcaaca ctgccagtac
    12181 cgtttcactt cctgcattac gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc
    12241 gattaaacct gctgcacgtc tgacggcaaa tggcattgtc ggattactgg caacccgcgg
    12301 aacagttaaa cgttcttata ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat
    12361 agaaatgctg ggctcggcag agatggttga gttggctgaa gcgaagctac atggcgaaga
    12421 tgtttctctg gatgcactaa aacgtatcct acgcccgtgg ttaagaatga aagagccgcc
    12481 agataccgtt gtattgggtt gcacccattt ccctctacta caagaagaac tgttacaagt
    12541 gctgccagag ggaacccggc tggtggattc tggcgcagcg attgctcgcc gaacggcctg
    12601 gttgttagaa catgaagccc cggatgcaaa atctgccgat gcgaatattg ccttttgtat
    12661 ggcaatgacg ccaggagctg aacaattatt gcccgtttta cagcgttacg gcttcgaaac
    12721 gctcgaaaaa ctggcagttt taggctgatt tggttgaatg ttgcgcggtc agaaaattat
    12781 tttaaatttc ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga
    12841 acaacggcaa acacgccgcc gggtcagcgg ggttctcctg agaactccgg cagagaaagc
    12901 aaaaataaat gcttgactct gtagcgggaa
//
LOCUS       DBBGLQ010000121        12924 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.121, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000121 DBBGLQ010000000
VERSION     DBBGLQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.121"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..260
                     /locus_tag="ACIYSB_004461"
     CDS             <1..260
                     /locus_tag="ACIYSB_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HGC5431369.1"
                     /translation="KANHYLDDADLLVVITLHGRFSQGWYFCFEAREFLETGDEAARL
                     AGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(298..2040)
                     /gene="ggt"
                     /locus_tag="ACIYSB_004462"
     CDS             complement(298..2040)
                     /gene="ggt"
                     /locus_tag="ACIYSB_004462"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGC5431370.1"
                     /translation="MIKPTFLRRVAIATLLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGLNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            2160..2600
                     /locus_tag="ACIYSB_004463"
     CDS             2160..2600
                     /locus_tag="ACIYSB_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGC5431371.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(2587..3330)
                     /gene="ugpQ"
                     /locus_tag="ACIYSB_004464"
     CDS             complement(2587..3330)
                     /gene="ugpQ"
                     /locus_tag="ACIYSB_004464"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGC5431372.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKMFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLNKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(3327..4397)
                     /gene="ugpC"
                     /locus_tag="ACIYSB_004465"
     CDS             complement(3327..4397)
                     /gene="ugpC"
                     /locus_tag="ACIYSB_004465"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGC5431373.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPMVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(4399..5244)
                     /gene="ugpE"
                     /locus_tag="ACIYSB_004466"
     CDS             complement(4399..5244)
                     /gene="ugpE"
                     /locus_tag="ACIYSB_004466"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGC5431374.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(5241..6128)
                     /gene="ugpA"
                     /locus_tag="ACIYSB_004467"
     CDS             complement(5241..6128)
                     /gene="ugpA"
                     /locus_tag="ACIYSB_004467"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGC5431375.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(6226..7542)
                     /gene="ugpB"
                     /locus_tag="ACIYSB_004468"
     CDS             complement(6226..7542)
                     /gene="ugpB"
                     /locus_tag="ACIYSB_004468"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGC5431376.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(7941..8654)
                     /gene="livF"
                     /locus_tag="ACIYSB_004469"
     CDS             complement(7941..8654)
                     /gene="livF"
                     /locus_tag="ACIYSB_004469"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGC5431377.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRIQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(8656..9423)
                     /gene="livG"
                     /locus_tag="ACIYSB_004470"
     CDS             complement(8656..9423)
                     /gene="livG"
                     /locus_tag="ACIYSB_004470"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGC5431378.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(9420..10697)
                     /gene="livM"
                     /locus_tag="ACIYSB_004471"
     CDS             complement(9420..10697)
                     /gene="livM"
                     /locus_tag="ACIYSB_004471"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGC5431379.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(10694..11620)
                     /gene="livH"
                     /locus_tag="ACIYSB_004472"
     CDS             complement(10694..11620)
                     /gene="livH"
                     /locus_tag="ACIYSB_004472"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGC5431380.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(11668..12777)
                     /gene="livK"
                     /locus_tag="ACIYSB_004473"
     CDS             complement(11668..12777)
                     /gene="livK"
                     /locus_tag="ACIYSB_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGC5431381.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         3030 a         3734 c         3311 g         2849 t
ORIGIN      
        1 cgaaagcgaa ccattacctt gatgatgcag atctcctggt cgtcattact ctacatggac
       61 gctttagcca gggctggtat ttctgtttcg aagcacgaga atttctcgaa actggagatg
      121 aggccgcgcg cttagctggt aacgcacctt ttattattga taaagacagt ggtgaaattc
      181 attctctggg aacggcaaaa ccgctggaag aatatctaca ggattacgaa ataaaaaagg
      241 ctaccttcgg cttgccctga caaaatagcc ctcttcccac gaagagggcc gctaacctta
      301 gtaccccgcc gttaaatcat ccaccgagcg cgggtcggat gcgccgtaca actcaccgtc
      361 cggcccaacc ataatgcttt gtgtactgcc catcgcctct ttcagcgcca ctttctgacc
      421 ttttgcttcc agcagcttga gcgtatccgg gctaaaccct ttttcgacac gcagctcgtc
      481 cggcaaccac tgatggtgga aacgcggcgc attggtcgct tcggcgacgt tcaagccata
      541 atcgatgcta ttcaccacca tttgcagcac tgtagtgatg atccggctac cgcctgggct
      601 accggtaacc agccaggttt taccgtcttt caccacaatg gtcggcgaca tcgacgacag
      661 cgggcgtttg ttcggcccga cggcgttggc atcaccgccc accagcccgt aaacgttcgg
      721 tacgcccggt ttggcggaga aatcatccat ctggttatta agcagaatac cgctctcgcc
      781 cgcgacaatg cccgtaccga aggtggtgtt cagcgtatag gtcaccgcca ccgcgttacc
      841 atctttatcc accactgagt aatgggtagt ttgattactc tcataaggcg caagcttgcc
      901 ggggcgaatt tcgctggatg gcttcgcttt attgatatca atttgatcgg caatagattt
      961 ggcataggct ttattggtca gcgcctgcca cggtactttg acaaaatccg ggtcgccaag
     1021 atattccgag cggtcggcgt aggcgtattt ctccgcttct gccatgattt gcatcgcatc
     1081 ggcgctgcca aagccgtatt tcttcatatc gaagttttcc agaatattga ggatttgtac
     1141 gatatggatc ccgccggagg atggcggtgg catggagtaa acctgatacc cgcgataatc
     1201 gccgcttatc ggagtgcgtt cgaccgcttt ataggctgct aaatcttctt tagtgatcaa
     1261 gccaccgttt ttctgcatct cctgggcgat ctgttccgca atcgtgcctt tatagaattc
     1321 gtccgggccg ttttcagcaa tcatctccag gctctttgcc aggttcgcct gcaccagcgt
     1381 gtcgcccttt ttcagcggct cgccctcttt ccagaagata gctttactgt tttcgtgatt
     1441 cggcaacact tcgctaccgt aggttttgag atcgtcagcc agcgcgtcgt taacgataaa
     1501 accatcgcgt gccagtttaa acgcgggctg cacgactttg ttcagcggca tggtgccgta
     1561 tttatccagc gccagcgaga aacctgctac cgtacccggt gtgccggaag ccagatgcga
     1621 agtgagtgat tttttgctgt ccgggttgcc ctgatcatcg aggaacatat cgcgggtcgc
     1681 tttggcgggt gccatttcgc ggaaatcgat agccgtggta ttgccatttt tcgagcggat
     1741 taacataaaa ccaccaccgc ccagattccc tgcctgcgga tgcgttaccg ccagcgcgta
     1801 gcccaccgcc acggcggcat caacggcatt cccgccctcc ttgagaatat ccacccccac
     1861 ctgagtggca gtggcgtcca cagacgctac cattccctgt ttcgcgcgta ccgggtggaa
     1921 gacatcttcc tccacaccat acgagacggg cggcgcagga ggcgcggcgg cggcgctaaa
     1981 acaacttcct gagagcagag tagcaatggc cacccggcgt aaaaacgtcg gttttatcat
     2041 cgttattctc cagagattaa ggggcaaccc cagctaagcc tgttatataa ctctgaatta
     2101 atcatcgttt tgtcgggaag cgagtaaact taaaggatat cttcagagga ggatacgcga
     2161 tgaaacgact tctgattctt acggcactcc tgccgtttgt cggctttgca cagcccatta
     2221 atactctgaa caaccctaac cagccggggt atcagatacc cagccagcag cggatgcaaa
     2281 cccagatgca gactcagcaa atccagcaaa aagggatgct gaatcagcaa ctgaaaacgc
     2341 aaactcagtt gcaacagcag catttagaaa accagataaa caataattct cagcgggtgt
     2401 tgcagtcaca gccgggggag cgaaatcccg cccggcagca aatgctgccc aacaccaacg
     2461 gcgggatgtt aaacagcaac cgtaatccgg atagttcgtt gaatcagcag catatgctgc
     2521 cggagaggag aaacggcgac atgctgaatc agcccagcac gccgcagcct gacattccgt
     2581 tgaaaactat tgggccgtaa agttcggacc aatcacgtca atcgcatcgg tacagatgca
     2641 atccacaccc cagcgcagca actctgctgc gcgctggggt ttattgacgg tataaaccag
     2701 aatccgcagt ccggcgtctt tcaactgcat cactcgcgct ttattgagta acttatgatt
     2761 gagatgaata gagacgcagc ccagccgcgc ggtcagttcg cgccagtcgt cgcgccactc
     2821 atccagcaac aaaccgcgcg gcagttccgg tgccgcctgt tgtgcagctt ctaaagcatc
     2881 aatctcaaac gatgacagca gcggcggcgt cataccggcc cacagctcgc gtgccgccag
     2941 cgccaccatt ttgcccgtta atggcccggt gccggtggtg ggtttgattt cgatattcgc
     3001 catcatcccg tgttcgcggc aacgttccgc cacctgcgaa agcaacggta gcggctcacc
     3061 tttaaacatt ttgctgtacc aactgcccgc atccacgcgc agtaaatcct gccagttcag
     3121 ttcacccgcg acgccccagc cgttgctggt acgttcgaga ttgtcgtcat ggagcaggaa
     3181 gatctcgcca tctttcgata acttcgcgtc aaattcgatc atcttatgac cgtattttgc
     3241 cccgacgtcg attgccgcca gggtgttttc cggggccagc ttaccgccgc cacgatgagc
     3301 gacgatgcgg ggataaggcc agttactcat actcgttgtc ctgtttcacc atcaaaaaga
     3361 tgcagctgat tttccgccag atgcagccac agcgtgctgc ctgccgtcgg gcgctcctga
     3421 tgcgccagtc gcaccaccag cttctgctcg ccccagcgtc cgtgcgccag gttatctgcg
     3481 ccgaggatct ccagcgtgtc catcaccatc ggtacgccgc cttctgcctg cgagcttagc
     3541 gcaatatgtt ccgggcgaat gccgagagtc attttacgcc cggcatactg acggtagcca
     3601 ccgtttagcg gcagctcaat accgccgtcc agttcgaaat gcgtgccttc gttattcacg
     3661 cggcctgtca gcaggttcat cgccggactg ccgataaaac tcgctacaaa caggctggcg
     3721 ggcttttcgt agacttcaac tggtgtgcca atctgttcgg caacaccgcc gttcatcacc
     3781 attactcgct gggcgagcgt catcgcttca acctgatcgt gagtaacgta gagtgaagtc
     3841 gttttcaggc gacggtgcaa ctgttgcagt tcaagacgca tctgcacgcg cagcttggca
     3901 tcgaggttag agagcggctc atcaaacagg aacaccgccg gatcgcgcac aatcgcgcgg
     3961 cccatcgcca cacgctggcg ctgaccgccg gaaagctcgc gcgggcgacg tttgagcaga
     4021 ccgtccagct ccagaatgcg cgccgcttct ttaacgcgct cggcaatttg ctgcttgccc
     4081 atgccgcgaa ttttcagccc ccacgccatg ttttcttcga cactcatatg cggataaagc
     4141 gcgtagttct ggaacaccat cgcaatcccg cgatcttttg gctccatttc agtcacgcgc
     4201 tggtcgttga tccagatatc gccttctgtc acccgctcca gcccggcaac catgcgcagc
     4261 agcgtcgatt tcccgcagcc agacggcccg accatcacga taaattcgcc atccgccaca
     4321 tcaagggtca gcggtttaat gacctgggtt ttaccatccc agcttttggt tactgcctgt
     4381 agtttcagtc ctgccatctt atttctcact atcgaccagg ccgcgcacga aggcacgctg
     4441 catcactaaa acaatcacca ccggagggat aagcgttaac aacatcgccg ccatcactga
     4501 gttccattcc gtggtgcctt cgcctgtagc gatcatccct ttgatccctg ccacggtggt
     4561 gccgagatcc acatcggtaa taatcaacaa cggccacaaa tactgattcc agccgtagat
     4621 aaaggtgatc acaaacagcg ccgccagatt agttttggag agcggaaaaa cgatgtcgca
     4681 aaagaagcgc attggcgatg cgccgtcgat ccgcgcggct tccaccagct catccggcag
     4741 cgtcataaag aactggcgga acaggaaagt agcggtcgcc gaggccatca gcggcagcgt
     4801 taaaccggcg tagctgtcga gcatctgcag gttggcgatg acttccaccg tcgggaagat
     4861 acgtacttca accggcagca tcagggtgat aaaaatcatc cagaagaaga ggttacgtag
     4921 cggaaaacga aaccagacaa tggcaaatgc cgagagcatc gagacggtaa ttttgccgag
     4981 cgtaatgctg aacgccatca caaagctgtt aagcaacatc cgccagaacg gcgcgctatt
     5041 cgtgcctacc ccgttcaccc agatgttgtg gatgttttcc agcagatgtg tgccggggat
     5101 gagcgtcatc ggcgcggcat agacggcctg tttatccagc gtcgccgcga caaacgccac
     5161 gtacagcggg aagaggatca ccgcgatccc gaggatcagc atggtatggc tgaatatcgt
     5221 cagccacgga cggttctcaa tcattggtaa cgcaccttgc tttcaacata gcggaactgc
     5281 accaccgtca gcacgatgac gaggaacatc aacaccaccg actgtgcggc agacgaagcc
     5341 agatccagtc cggtaaaacc ttcgcggtag atcttataaa tcagcgtcgt ggtggcctga
     5401 accggcccgc cggacgtggc ggcgtcgatc accgggaagg tgtcgaagaa ggcatacacc
     5461 aggttcacta ccagcaggaa gaaactcacc ggggcgataa gcggcagcgc aatcttaaag
     5521 aagcggcgaa tcggccctgc accgtcgatg gctgcggctt cgatcaacga acggggaatg
     5581 gattgcagcg cggcatagaa gaacaggaag ttgtagctga tttgcttcca tactgaggca
     5641 aacaccacca gaaacattgc ctgaccgctg ttttgcgcgt ggttccagtc gtagccgaac
     5701 tccgcgagaa aatgggtgat cagcccgcga ccggggttaa acaggaagat ccacaatacg
     5761 gcggcaacgg cgggagccac ggcgtaaggc agcaacatta aggtttgata gaaacggctg
     5821 ccgcgcacga tgtactccac cagcgccgca aagaacagcg ataccagcaa accgctgacg
     5881 gtgacaaagg tgctgaattt tatcgtcgtc cagaaggagt cgagatagta gctgtcatgg
     5941 aacagcgtga cgaagttatc caggccgaca aactggctgg agaaaccaaa cggatcgacg
     6001 ctttgtagcg agtaccacaa cgcttcgccc gcaggccaga taaaaaagat aacggtgatg
     6061 atgagctgcg gcgcgaccag cagataaggc agccagcgcg agcggaacac cggacgggat
     6121 gatgacatta cggttaattc ctgaactgtg ccggatgcgc ttcgcttatc cggcctacac
     6181 gcgatgccag gtcagataag cgaaacgcat ccgacattac actgattaag acttcgtcga
     6241 tttctcaaag cggcgcagca actgatttcc acgctcaacg gcggtatcca gtgcctgctg
     6301 tggtgtcttc ttaccggtcc acacgctctc cagctcttca tccacaatca cgcggatctg
     6361 cggcatgttg cccagacgca gccctttggt gaacggcaac ggcggcttat tcagcatctg
     6421 acgcgtcgcg gtatccgccc ctgggttttt ctcataaaag ccctgctcac gggtcaggtc
     6481 atacgctgct ttggtgattg gcagataacc ggttttctga tgccactcgg cagcgttttc
     6541 tggcttcgcg aggaaatcga ggaacttcgc cacaccggta tacgtttctt tatctttacc
     6601 ctgcatcacc cacaggctgg ctccgccgat aatggcgttt tgtggcgcat ctttcgcatc
     6661 ggcgtcgtaa ggcatcatgc ctacgccgta gttaaatttg gcgtactcgc gaatgttggc
     6721 aagagaaccg gaagaggcgg tggtcatcgc gcaatcaccg ttatagaact tctcggtgga
     6781 ttcatcctta cgaccgacgt agctgaagtc gcccttcttg ttcatctcct cgagcatggc
     6841 gatgtgtttc acctgctccg gcttgttgaa ctccagcacc gcgtccgtgc cgtcaaagcc
     6901 gttgtttttg ctggcaaacg gcagaccgtt ccaggcgcta aagttttcca gttggatcca
     6961 gccctgccag ccgctggcgt agccgcactt catgccggag gctttcagtt tcgcggcata
     7021 gtccgccaga tcctgccagg ttttcggcgg ctgttccggg tctaatcctg ctttcttgaa
     7081 ggcgtctttg ttgtaataga gaacgggggt cgagctgttg aatggctggg agagtaagtg
     7141 gcccgttttg ctgtcggagt agtaacctga aaccgtcggc acaaactgcg actcatcgaa
     7201 ctgaatccct gcctctttaa acacgtcata caccggttta atggctttcg acgccatcat
     7261 ggtggcggtg ccaacttcat aaacctgcaa aatagccggc gcgttgccgg tacgaaatgc
     7321 ggcaatcccc gcgcttaaat tctgttcgta gttgccttta taggtcggta caattttgta
     7381 atccgggttt tcggcgttaa aacgttgggc cagagaatcc acctctttac ccagttcccc
     7441 ttccatagaa tgccagaacg gaatggtcgt cactgcctgt gcattcccca ttaacgccag
     7501 tccgagcgcc agtgctgaag ctgtataatg taacggtttc atcgtttatc tctcttgttg
     7561 taccgaatgc gcgaattcac gcgttttatg ctcgcggggt aacatgacat gctcgaatta
     7621 cagaaaaata acttttttgt tacatttgta agatagtaag gtgtcagaaa gatgacaagg
     7681 cggtgacggc gtgggtgagg gaaaatggga gatggggcac ggataagcgg gaaaatatag
     7741 aaggtctgaa tcaaactcta cagattgctc atcgtttcat gccggatgcg gcgtaaacgc
     7801 cttatcaggc ctacaagatc gtgcaaattc aacatattgc cactcaccca gtaggcctga
     7861 taagcgcagc gcatcaggca attttacatt tgtcacctgt ctcaaaggag tcttttgact
     7921 ccctatcaat caacgtgtta ttacccgcct aaatacgcac ttctcaccgc ttcattcgcc
     7981 agcagcgcat caccagtatc ggaaagcact acatggccgt tttccagcac gtagccgcga
     8041 tccgccagct ttagcgcctg gttggcgttc tgctcgacga gaaagatagt catcccctgc
     8101 tcgcgcagct gctcgatggt gtcgaaaatt tgctggatga taatcggcgc aagaccgagc
     8161 gatggctcat caagcagtag caaacgcggg ttgctcatca gcgcacgacc aatcgccagc
     8221 atctgctgtt caccgccgga catggtgccc gcccgctgaa tacggcgctc atgcagacgt
     8281 ggaaacagct catacaccca ctttatgcgc tcctggaact ggtcgcgttc agcaaaaaaa
     8341 ccgcccatcg ccaggttctc ttccaccgtc atccgcgaga agacgcgacg cccttccggg
     8401 acaatcgcca ccgcttcgcg catgattttc gctgtctgcc agtcggtaat gtctttatca
     8461 tcaaacacaa ttcgcccgct ggtggcacgc ggatcgccgc ataacgtgcc gagcaaggtg
     8521 gttttccccg ccccgttcgc gccaatcagc gtgacaatct cgccctgatt gatatgcagg
     8581 ctcacctcat gcagcgcctg gattttgccg tagtgggcgc tgactttgtc aaaggacaac
     8641 atgacttttt ccatcttatg cctcacctaa ataggcacgg atcacgtccg ggttattacg
     8701 gatctgctcc ggcgtaccgt ttgccagcgg cgtcccctga ttgaccacgt aaattcggtc
     8761 cgaaattccc atcaccagct tcatatcgtg ttcaatcaac aagatagtgg tgttgtgatg
     8821 attgcgcagt tcggcaatca gctcatccag ctctttcgtc tctttcgggt taagacctgc
     8881 cgcaggttcg tcgagcatta aaatctccgg ctgcgtcacc atgcagcggg caatctcaag
     8941 acggcgctgg tcaccatagg ccaggttact cgcctgacgg ttggcgtgtt ccagcaaacc
     9001 aatgcgctca agccaggtcg cggcgcggtc gagcgcttcg ctctgggcgc gacggaagga
     9061 tggcgttttc aacaggccag agaacagccc ggttttcagt tgctgatgct gcgccaccag
     9121 caggttttca attaccgtca tttcacggaa cagacgcaca tgctggaagg tgcgcaccac
     9181 gcccatgcgg gcaatttgct gccccggtaa accttccagg tgctgatcgc gcagtaaaat
     9241 ggtgccgccg gtgggtttgt agaatccggt cagacagtta aaaaccgtgg tttttccggc
     9301 accgttaggg ccgattaacg agacgatctc ctgcgggtac agttcaagat tgacgttgtt
     9361 caccgccagc aggccgccga agcgcatcat caggccgtta acagataata atggctgact
     9421 catgcctgct ctcctttcgc tgcgccgttt ttcagcttca gttgcgggcg cgtcatgggc
     9481 agcaagccct gcggacgcca gatcatcatc agcaccatca aaccaccgag cattaacatg
     9541 ctgtattcgt tgaaatcacg catcaactcg cgcgacacca ccagcaaaat tgccgccaga
     9601 atcaccgcaa attgcgagcc cataccgccg agcaccacta tcgccagcac aaacgccgat
     9661 tcggcaaagg tgaaggattc cgggctgaca aagccctgac gcgccgcaaa cagcgttccg
     9721 gcaaaaccgg caaacgcggc acttatggta aaggcagtca gcttgatacg acgcgggctt
     9781 aagcccagcg aacggcaggc gatttcatct tcacgcaacg cttcccacgc acgccccagc
     9841 ggcatccgca gcaggcggtt aatgacaaac aggcttagca ccaccagcag caacgccacc
     9901 aggtagagga agatgacacg atcggaggga tcgtatttca ggccaaagaa attactgaac
     9961 gtgtcccagc cgccttcacg agcggtacgg ctgaactcga gtccgaagag tgtcggtttc
    10021 gggatctgac tgattccgtt cgggccgccg gtaatttcgg tgttattgag cagcaatatg
    10081 cgcacaattt cgccgaaacc gagggtaacg atcgccagat agtcaccgcg caaacgcagc
    10141 accggaaaac cgagcaggaa gcccgccgcc gctgccatta atccagcaat cggcaggcag
    10201 gtccagaagc ccaagccgta atagtgattg agcagcgcaa aagtgtaagc gccgatggcg
    10261 taaaaaccgc cgtaccccag caccagcaga ccagaaagac caacaaccac gttcagcccg
    10321 agaccgagga taatgtagat catggtcagg gtggcaatat ccaccgtccc gcgtgaaacc
    10381 ataaacggcc acgccaccgc aagcaccaac agcgccacga ggaacagttt ctgcttcacc
    10441 gtggagccat caatggcggg cagaataaac ttcggtccgg aaacgctttt caaccctttc
    10501 tggaaagccg gtcgcaaaag ctggaagaaa aagaccaccg ccgtgccgat aaacacccac
    10561 tgccaacgga catccgaagc cgtgtcgacc accagtttgg tgccatccag ctccagttgc
    10621 acgcccataa agacgcccgc cagcacaaag aacatcgcgg cagagagcag cgccattgca
    10681 atatgcatcg gtttcatact ttctctacct ccgggcgacc cagaataccg gtcggcatca
    10741 ccagcagcac cagaatcagc agggcgaatg agaccacatc tttatattcc gtactcagat
    10801 aggcagaaga gagcgcctcc gcaatcccca gaatcaggcc gccaatcatc gctcccggaa
    10861 tgctgccaat cccaccgagc accgccgcgg taaaggcttt catcccggcc ataaagccga
    10921 tgtaggggtt aatgacgccg tagaactgac cgagcagcac acccgccacc gccgccatcg
    10981 ccgcgccaat cacaaaggtc agcgcaatca cccggtcggt gttaatgcca agcagactcg
    11041 ccattttcag atcttccgcg caggcacgac acgcgcgacc catgcgggaa tagcgaatga
    11101 aaatcgtcag cgccagcatg gcgaggaagg taacaatcca gatcaccgcc tgcatggtgg
    11161 taatagaggc agagaagttt tcgctatgcc ccaccaccca ctgaccgtta aacaggctcg
    11221 gcagcgccac gtcgcgcgaa ccttcggtca ggctgacgta gttttgcagg aagatggaca
    11281 taccgattgc agagatgagt gcaatcaggc gcttagagtt acgcaccggg cggtaagcca
    11341 cccgttcgat actccagccg taggcgctgg caatgacgat tgcgccgacg aatcccgcag
    11401 ctaccagcag ccagccggta tcaatgccca tcatcatcag cgcggcgatg atcataaatg
    11461 agacgtagct gccaatcata taaacctcgc cgtgggcgaa gttgatcatg ccgataatgc
    11521 cgtaaaccat ggtgtagccg atggctatca gcgcgtaggt actgcccagc gtgacgccgt
    11581 taaacatctg ctgcaagaaa tacaaaaact gctcagacat aaggtaacct ttctaaaccc
    11641 gcccgcattt tacgggcggt gggatgatca cttggctgcc gtggatgaac cgtcggcgtg
    11701 ccactggaag acaccaaaat caaatccctt aagatcgcct ttttcatccc agttcagcgg
    11761 cccaatcacg gtgtttgcac cgttagcttt taaatctttc accagcgcca gcggctcatc
    11821 gctgccggta cgctcaaggg ctgttgccag agattgcacc gccgcgtaag tgatccagac
    11881 ataaggcccg gacggatctt tcttgtctgc tttcagcgca tcaacgatgc cctggtttgc
    11941 cggatcctgg tcatagcgtt ttggcatggt gaccaacatg ccttcggcag catcgccagc
    12001 aatattcgac agcgacgcat tacccacacc ttccggcccc ataaactggg ttttcaggcc
    12061 aacggaacgg gcctggcgca gcatctgccc catttccggg tagtaaccgc cgtagtaaac
    12121 gaagtcgatg ttttcttttt tcaggcgggc gatcagcgcg gagaaatctt tctcgccagc
    12181 ggtaatgccg tcgaagaaaa caacattggc gttagcagct ttcagcccgt cctgcaccga
    12241 acgtgccagc ccttcgccat attgttgttt gtcgtgaatg atggcgatgc gctggggctt
    12301 caccgtctca agaatgtatt ttgccgccgt tggcccctgg gaagagtcca gcccggcagt
    12361 acgcataatg tgttgataac cgcgttgggt cagctccggg ttggtcgctc ccggcgagat
    12421 catcagaata ccttcgtctt catagatatc tgacgcaggc tgggtagaag aagaacacag
    12481 atgaccaata acgtatttaa tgccgtcatt aacgattttg ttggcgaccg caacggcttg
    12541 tttcgggtcg caggcgtcgt catattccac gccaaccagc ttgtcgccct taattccccc
    12601 tttggcatta atgtctttaa tcgcctgacg cgcaccgtta aattccatat cgccccactg
    12661 ggcaatcggg ccggacatcg cgccgacaac ggcgacttta atatcgtcag ccatagcggt
    12721 gtgtgaaatt gccagtgcaa tcatccctgc gatgatagtt ttcgcattcc gtttcatagt
    12781 caaaaatccc cattcgtgat gttgtgttgc tttgttttta tgtgttaaca aatcagactg
    12841 ttcttttttt atactgcact gtttttgcct gtctgattaa gggtttagcg cagtattttg
    12901 tgataatagt gattaaaatc ccta
//
LOCUS       DBBGLQ010000122        12904 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.122, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000122 DBBGLQ010000000
VERSION     DBBGLQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.122"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            502..651
                     /locus_tag="ACIYSB_004474"
     CDS             502..651
                     /locus_tag="ACIYSB_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431382.1"
                     /translation="MKPEHIHRLTGRDVLRYRRKHFDLMTGLVVATALGLIITFILLV
                     ARATV"
     gene            648..1577
                     /locus_tag="ACIYSB_004475"
     CDS             648..1577
                     /locus_tag="ACIYSB_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431383.1"
                     /translation="MSLETSLELNNKLLAQHNVLLERLIRTMASGIVMHPDTISQVQE
                     CRDTASETAVETETATETENKAAMTLDDLDFSDVIALAAFYPVPQQITEDMLQRAVAY
                     RDAEGDKRVVQIDALDSTLQGVKRARALHKPALLDLSRNILKFWDDLPTIGERRAFAE
                     QLLDAPADGRDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIE
                     INRVEYLQLQEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEA
                     IAILEKQGARKLGEVTDENLAEVITLAEKALEG"
     gene            1580..2881
                     /locus_tag="ACIYSB_004476"
     CDS             1580..2881
                     /locus_tag="ACIYSB_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HGC5431384.1"
                     /translation="MPDVHAQLSPSSAHRWMRCPGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDKSGPQVDDEMVEAVGRYVDTV
                     WTLAKNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERALEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDFKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLTADLI"
     gene            2897..3445
                     /locus_tag="ACIYSB_004477"
     CDS             2897..3445
                     /locus_tag="ACIYSB_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HGC5431385.1"
                     /translation="MKLKLNNVRLAFPSLFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            3497..4138
                     /locus_tag="ACIYSB_004478"
     CDS             3497..4138
                     /locus_tag="ACIYSB_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HGC5431386.1"
                     /translation="MSQPVLTNHLKTQIINNALEKAGIPKRKSALRAARVEWAERVRL
                     AAIGGVEKEAEILKNLKKIESMVTKIPDSLKTNSSLIRKDCCIYLNLAGARANIYFNG
                     NYRGYESGAPEHINKIAPAEFTLLGEDPLVTEFYSFDALYTQIKSDETEIIQNVSAAL
                     NKVRTVKRLLDVWPEAKELLPTDAPPVPLAPAVHRETLNEMIGLPTNTVSDTQ"
     gene            complement(4132..4821)
                     /locus_tag="ACIYSB_004479"
     CDS             complement(4132..4821)
                     /locus_tag="ACIYSB_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431387.1"
                     /translation="MTVGNKRQPNGLPEYYCHRGNDIKDAIVRDFIIQPVAHLKLITG
                     QTKTSYSGYPLTDQYYCFSFKKRSDENCTGAFFCGRDVANDFIRRIGCTELPLFNPMK
                     SDTSAAIERSGSGSGSSGITVKPNADKKLFIDAARLLMTLWDSTSKGQTPLASVLNEA
                     ISGGIDKPPEIRHVVAVNTMLGNTKNTLTGLLDKARAKYPDVLFRDFDFTPLNEIVKR
                     SKSYNKHPPCY"
     gene            4883..6946
                     /locus_tag="ACIYSB_004480"
     CDS             4883..6946
                     /locus_tag="ACIYSB_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HGC5431388.1"
                     /translation="MSNILWGDLETYCDIPINNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIADPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTEQALWHRDQQINDRGVCMDMQLARAAIEAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYDESVKRKSTYGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEKGNITYMGVNSYSRKWQRLKTYGGKLVENITQAAARDILAGN
                     MPLIEDAGYSIVLTVHDEVITEAPDTEDFNDKALSALLSTDPEWAPDIPLNAGGFEAY
                     HYKKD"
     gene            7011..7424
                     /locus_tag="ACIYSB_004481"
     CDS             7011..7424
                     /locus_tag="ACIYSB_004481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431389.1"
                     /translation="MQELTEMPITYPYPRETFPDFLRECGFTNFSHEQRETCDCYRLT
                     NGVIVNLYTTRTIQFQGNPQERPNVETAIMTHLGTPPTATPLAVEQPSELPKKIFIVH
                     GHDHTAKEQLELILHKLGLPDHFILQNGRDWTHHY"
     gene            7396..7770
                     /locus_tag="ACIYSB_004482"
     CDS             7396..7770
                     /locus_tag="ACIYSB_004482"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050135 - NADP+ nucleosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HGC5431390.1"
                     /translation="MGGTGLTIIEELEREIGQGQTATRFGIVLLTPDDMGYSKRAGEA
                     EIQPRARQNVVLEMGMLLSSLGRSNVAILQKQHLEQPSDANGILYLNFNDHVRETVPR
                     LVQRLQNSGFEFTQAQIANASS"
     gene            7834..7968
                     /locus_tag="ACIYSB_004483"
     CDS             7834..7968
                     /locus_tag="ACIYSB_004483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431391.1"
                     /translation="MSFKYRDSPLYYRTVREALHLEQSGGYDRAMQVWAKANVGLIFV
                     "
     gene            8005..8199
                     /locus_tag="ACIYSB_004484"
     CDS             8005..8199
                     /locus_tag="ACIYSB_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431392.1"
                     /translation="MSNIDNKGWGFPALSKKAHFFNSGEVISLCGKWMFIGIRIDEWH
                     DHPENCAICMKKRKKQEGES"
     gene            8199..8486
                     /locus_tag="ACIYSB_004485"
     CDS             8199..8486
                     /locus_tag="ACIYSB_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HGC5431393.1"
                     /translation="MAYERENLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIIFVECKSPGKPPRPDQLREHGRLRALGFTVVVLDSKNLEAIILSRNKG"
     gene            complement(8499..9296)
                     /locus_tag="ACIYSB_004486"
     CDS             complement(8499..9296)
                     /locus_tag="ACIYSB_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431394.1"
                     /translation="MPLAKIFTKQVSLLTMFSALAYAMAYSFQKGVASYYGYPDLFIK
                     IDLNTLLYSAVWLLFFSFLFLGALYAALMFDLKGKYYVPFIVFIVVMLMANICIIGFH
                     WPFGYFSETSVMSVQDILLFAMLLLNYHNVVSMPIHSKRHEHHMESIRKFGRLAEYKN
                     VFYYIDLKILKSAFPLGVITLLILSYTFGKVTAFKNNEYYMVDGSNTKVLLNSFGSGF
                     VVGDCESNSAKFEFVNDLKGMRFTIMSSKKEIAKLKECFEIRVKNTD"
     gene            9370..10764
                     /locus_tag="ACIYSB_004487"
     CDS             9370..10764
                     /locus_tag="ACIYSB_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HGC5431395.1"
                     /translation="MNSIFHPRIYQNLIINHQTNILRGNIWAGMGMGKTVATLTALED
                     LFMAGAETRPALVLAPLRVAASTWPDEALKWGHLRNIEVQPIIGNAKARSMALTNSNA
                     SVFTINYDNLVWLIEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVR
                     RWINLTGTPAPNGLVDLWGQSWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQGEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDIKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPKTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            10761..10961
                     /locus_tag="ACIYSB_004488"
     CDS             10761..10961
                     /locus_tag="ACIYSB_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5431396.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNIDAFIQKCRSQY"
     gene            complement(10958..12355)
                     /locus_tag="ACIYSB_004489"
                     /pseudo
     CDS             complement(10958..12355)
                     /locus_tag="ACIYSB_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            12570..12830
                     /gene="yfhL"
                     /locus_tag="ACIYSB_004490"
     CDS             12570..12830
                     /gene="yfhL"
                     /locus_tag="ACIYSB_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGC5431397.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT         3677 a         3126 c         3033 g         3068 t
ORIGIN      
        1 tacaataaac atagtaattt atccctcatc aagtcgaacg gcgcgactct aaaccatgcg
       61 tcgggaccgt ggcgggacag gatgtcggca atacgggtca gtaagttccc tttggggtgc
      121 ggcgaagcac tgtctactga tacaagtcga agccgccgaa ccaccaaagt gaactgaatg
      181 aggaaacggc gtgaaaacgt ataaaccgct caaaggtgaa tgccctgctt gccaaacaca
      241 acttcagaca ccaaagccaa aacgatacca aatcaacgaa tgttatgagc actgccctag
      301 atgtggtgct ttcctgcata ccattgcaac gcactggcgt gtccgcttca acctcgtggt
      361 accacgtacg cacaatacca actgaatcat ccatgtaatt gctgtgtgta gtctttgccc
      421 gccgcaagtg acgggctttt ttatgtctga aagcgcactc gcaacagcgt gctccccgat
      481 atgaaaaaag gaatacaacc gatgaaacct gaacacatcc atcgactgac ggggcgcgat
      541 gttctccgtt atcgccgtaa acacttcgat ttgatgacag gtctggtcgt tgccactgca
      601 cttggcctga tcatcacttt cattctcctt gtagcgaggg ccacagtatg agcttagaaa
      661 ccagtctcga acttaataat aaacttctgg cgcaacataa tgtgctgctt gaacgtctta
      721 tccgcacaat ggcgtcaggt attgttatgc atcctgacac gatttcacaa gtacaggaat
      781 gtcgggatac agcatctgaa acagctgttg aaactgaaac agcaactgaa actgaaaata
      841 aagcggcgat gacactggat gatctggatt tcagcgacgt tatcgcactg gcagcgttct
      901 acccggtacc gcagcaaatc acagaagaca tgctgcaacg tgctgttgcc taccgtgatg
      961 ctgaaggtga taaacgagta gttcagattg atgctctcga cagcacattg cagggcgtca
     1021 aacgagccag ggcactgcat aaacccgctc ttctggacct gtcccgcaac attcttaagt
     1081 tctgggacga cctgccaact atcggcgagc gacgtgcttt tgccgagcag ctacttgatg
     1141 cacctgcgga tgggcgtgat gaagttaagc cgaaaaaggc cagtaacaaa gatggagaac
     1201 gcacggggcc gttttacgtc aaaaatgtat ccggcacagc agccagtgaa ctccacacct
     1261 tacgcaagtt gaacgagatg cttaaaaaag gccatatcga gatcaaccgt gttgagtacc
     1321 ttcagctgca ggaagaattt gcacgcagag acgcagcaaa ttccagccag aataatgacg
     1381 ccaaagatga ccatacaatt gatttcgcgg cactacgcaa acaagctgaa gggttgatcc
     1441 tccagttagc aaaagggggt taccgggcag aagctattgc aattctggaa aaacagggag
     1501 ccaggaaact tggtgaagta acggatgaaa atctcgctga agtaatcacc ctggctgaaa
     1561 aagcactgga gggttaatca tgccagacgt tcatgcacaa ctttctccat catcagcgca
     1621 tcgatggatg cgctgcccag gaagtctggc gctggaggcc acacaaccgg ataaagaaac
     1681 aacttttgca atcgagggca ctgcagcgca cgcgcttgct gaaaaagttc tacgaaacag
     1741 gcaaagccac ccggaacact acgccggatg caatgtttct atgtttctcg gctcataccc
     1801 ccttcgcgaa aatcctgatg ataaatctgg cccacaggtg gatgatgaaa tggtcgaagc
     1861 cgtcggccgg tatgttgata cggtctggac tcttgcaaag aataatgaac tattggttga
     1921 acaacgtgtt gatttctcac atataacggg tgtggaagaa tctttcggaa ctgccgacgg
     1981 cataatcatc gctggtaacg aattacaaat ccacgacctg aaatatggca aaggcgtccg
     2041 cattgatgca gaacaaaatg agcaactaca actgtatgct ctgggtgcgc ttgaacaatt
     2101 cagcatgctg tatgactttg aaactgtaag attatttatt caccaaccaa ggctcaacca
     2161 cgtttcagaa tggtcgttaa ccgtacagga acttcagtct ttcggtgaac gggcgctgga
     2221 ggccgcaacc agtgcgatcc ttgttctcaa tattgctgaa tgcgaaggca ttgagacact
     2281 accgctggaa aacttcatac ctggagaaaa acagtgccgc ttctgtaaag caaaagctat
     2341 ttgcactgcc cagaaaatgc agcatttaca aacagcggcc agcgattttg aagatctgac
     2401 aaagcctgtc agcgaaataa tcaccaatgc cagcgcacgt gtacctctgt taaccattga
     2461 gcagcttgca gagatctata gccaggccga ctttattgaa tcttggctaa aggcagtacg
     2521 ggaccgggtt cacaatgaac tcaatgccgg acatccggta ccggggttta aactggtaac
     2581 aggaaaacaa ggtaaccggg cctggagtga tgaagaggca gctcgcgcac ttctgaagga
     2641 ccagttcagg tacaaaactg aagaagtatt cgactttaaa cttattagtc ccacaaaagc
     2701 cgaaaaactt atcaaaaagg ccagtccgcg ccgttggtca aaagtcgagg cactgataac
     2761 acgagctgat ggtaaaccca ccgtcgttcc cgagtcagac ccacgccccg cactcaatat
     2821 caaccctgta aatgatttcg acgacgtatc cgacgatacg ctaaccgcag acctcatctg
     2881 atttaaggaa atccccatga aactgaagct gaacaatgtt cgtctggcct tcccgtctct
     2941 gtttgaagct aaaactgtaa acggcgaagg cgatccgcgt ttctccgcag tatttttaat
     3001 gtctcccaaa cacccacaac tggaagaaat ccgtaaagct atgaagcagg tagcgaagga
     3061 aaaatggggg gagaagtggg agtccattta taaccagctg gagaaaaaac tcaatctgtg
     3121 cctgcatgat ggtgatgaaa aagcagagta tgaaggcttt cccggcaatt tcttcctgaa
     3181 cgctgctaac aaagcgcgcc cagctgttct tgatcgcgat cgttcgccac taattcaggc
     3241 tgatggacgt ccctatgcag ggtgctatgt aaacgccgtt atcgatatct gggcacagga
     3301 caataatttc ggtaaacgca ttaatgcctc actcggcgga gtccagttcc tgcgagacgg
     3361 cgatgcattc gctggcggcg gagtggcaag cgctgacgat ttcgacgata tcagcgaagg
     3421 tgctgatgct gaagcactaa tttaaaaata ttcatccccc agctacatgc tgggggatct
     3481 catgagggaa agaataatgt cacagcccgt attaactaat caccttaaaa ctcaaatcat
     3541 caataacgca cttgagaaag cgggaatccc caaacgtaaa tcagcactac gagctgctcg
     3601 tgttgaatgg gccgaacgtg ttcggcttgc agctatcggg ggcgtagaga aagaagctga
     3661 aatattaaaa aatctaaaaa agattgaatc catggtaaca aaaatccctg attcattaaa
     3721 aaccaatagt tctttaattc gaaaagattg ctgcatatat ctcaatctgg ctggagccag
     3781 agcaaacatt tatttcaatg gcaactacag aggttatgaa tcaggagccc ccgagcatat
     3841 taataaaata gcacctgcag aatttactct acttggcgaa gatcctctgg taacggagtt
     3901 ttatagcttt gatgctctct acacacaaat taaaagtgat gaaaccgaaa tcatacagaa
     3961 cgtcagcgcg gctctaaata aagttcggac agtaaaacga ttactggatg tatggcccga
     4021 agctaaagaa ctgcttccaa cagacgcacc gccagtccct ttagcaccag cagtacatcg
     4081 agagacatta aatgaaatga taggccttcc taccaataca gtgtctgata ctcaataaca
     4141 agggggatgc ttattatatg attttgaacg ttttacaatc tcgttaagag gcgtgaaatc
     4201 aaaatctctg aataacacat caggatactt cgctcttgct ttatccagca ggccagttaa
     4261 agtatttttt gtgttaccca acattgtatt aaccgccact acatgtctaa tttctggagg
     4321 tttatctata ccaccagaaa ttgcttcatt aagaaccgac gccagtgggg tttgtccctt
     4381 acttgttgaa tcccacagcg tcattaacag acgagctgcg tctataaata gctttttgtc
     4441 agcatttggc ttgacagtaa taccggaact acctgagcca gaaccggaac gttctattgc
     4501 tgcactggta tccgatttca taggattaaa taacggcagc tctgtacaac cgatacgccg
     4561 aatgaaatca tttgcaacat ctctaccaca gaaaaaagcc cctgtacaat tttcatcaga
     4621 gcgtttttta aatgaaaaac aatagtactg atcggttaac ggataaccgg aataactggt
     4681 tttagtttgt ccagtaataa gtttcaggtg tgcaacaggc tgaataataa aatcccgaac
     4741 tatcgcatct tttatgtcgt taccacggtg acaataatat tcaggtaatc cattaggctg
     4801 tctcttatta cccacagtca ttagttgtct cctttaattc atgatatcta gcatttaacc
     4861 atataccata tgaggatatt ttatgtccaa tatactatgg ggcgacctgg aaacctattg
     4921 tgacattccg atcaacaacg gtacccatgc gtatgccgaa ggcgttgaag tgatgctttt
     4981 cgcatgggct atcaacgacg ggccagtaaa cgtgtgggat atcactgccg gtggtggtat
     5041 tccacacggc ttatacgaag caatcgcaga ccctgaaacc ctgctttatt tccataactc
     5101 tcacttcgac cgcaccgttc tgcgttatgc aatgccgcga ctggcaccgc cagtcgaacg
     5161 ttggcgcgac acgatggtgc aggcgctggc gcacggtctc ccggggtctc tgggggaact
     5221 ctgcgaagta ctaggcgtcc cgcaagacaa agcgaaggac aaagaaggta aggcgctgat
     5281 ccagctgttc tgtaagccac gtccgaaaaa tagcaaactg cgccgtgcca ccagcaaaac
     5341 ccacccggaa gaatggcggc gctttgttgc ttacgccgga ctggatatcg aggcaatgcg
     5401 cgaagtctat aaacgtctgc cgaagtggaa ttatcagggg acagagcagg cgctctggca
     5461 tcgtgatcag cagatcaacg accggggcgt ctgcatggac atgcaactcg cgcgcgctgc
     5521 gatcgaagcg gtagaccagg agcaaaagcg cctggcaaag cgtacacagg aaatgactga
     5581 tggcgaagtg caggcagcca cacaacgaga cgcgttgatt aagcacattg ttgaatccta
     5641 cggtgtggag ctaccagaca tgcaacgcag tactctggaa cgtcgtattg ccgaccccga
     5701 tttaccatct gccgtgaaag aactgctggc tatccgcctg caggccagta ctaccagcac
     5761 cagtaaatac aaggcactga tgaaaggcgt aagccacgac gggcgcttac gcggtacgct
     5821 acagttctgc ggggcgtcac gtaccggtcg ttgggccgga cggctattcc agccccagaa
     5881 ccttccccgc ccttcactaa aacaggaaca aatagacgaa ggcatcgaag cactgaaagc
     5941 cggatgtgca gacctgctgt ttgacaatat catggaacta accagttcag cgttacgtgg
     6001 ctgcattatc gcgccaacag gcaaaaagct ggtggtaagt gacttgtcga acattgaagg
     6061 ccgtatgctg gcatggctgg cgggagaaga atggaaactg aatgcattca gagagtacga
     6121 cgccggaacg ggtccggact tatataaact ggcgtatgca aaagctttcg atattgcacc
     6181 agatgatgtt gataaacaca tgcgtcagat cggtaaagtc atggaactcg gtctgggtta
     6241 tggtggtggt gtgtcggcct tcatcacttt tgcactggtt tacggtctcg atctcgacga
     6301 gctggcgaac gccgcactac caaacattcc ccgcgatgtt atccgcgagg cgaaaagctg
     6361 gtacgacgaa tcggttaaac gcaagtcaac ctacgggctt tctgaacggg tatttatcgc
     6421 ctgcgactca cttaaacgtc tctggcgccg ggcgcatccc gcgacctgtg atttctggta
     6481 cgaactggag cgcaccgtcc gcactgcaat cgccacaccg caaaaaacat tgtattgcgg
     6541 ttatcttaaa atccgccgcg atggcgcgtg gctgcgcata cagctaccat ccggacgcgc
     6601 tgtatgctac ccgtctccgg ttatcgaaaa agggaatatc acctacatgg gggttaactc
     6661 ttattcgcgt aaatggcaac gactcaaaac ctacggcgga aagctggtgg agaacatcac
     6721 ccaggcggcc gcccgcgaca ttctggccgg aaacatgccg cttatcgaag atgccggtta
     6781 cagcattgtg ctgacggtac acgacgaggt gatcaccgaa gcacctgaca cagaagattt
     6841 caacgataaa gcgctttccg cgcttctctc cactgacccc gaatgggcgc ccgatatccc
     6901 actgaacgct ggcggttttg aggcgtacca ctacaaaaaa gattgatata actcacctta
     6961 tcgagctatg gcgacatgct gaattcgagt aaattaaagg aacaatactt atgcaggaac
     7021 taactgaaat gccgatcacc tatccatacc cgagagaaac ttttcctgac tttttgaggg
     7081 agtgtgggtt caccaatttt tcccacgaac agcgtgagac ttgtgactgc tatcgtttaa
     7141 cgaatggcgt aattgttaac ctttacacca cccggaccat tcagtttcaa ggtaaccctc
     7201 aagaacgtcc caatgttgaa actgcaataa tgacccattt aggaacgccg cccacggcaa
     7261 caccactagc cgtagaacag ccatctgagc tccccaaaaa gatattcatt gttcacggcc
     7321 atgaccatac tgcaaaggag caacttgagc taatccttca taaacttgga ttaccagacc
     7381 atttcatttt gcagaatggg agggactgga ctcaccatta ttgaagagct tgaacgtgaa
     7441 attgggcaag gacaaactgc tacccgtttc gggattgttc ttttgacacc tgatgatatg
     7501 ggatactcaa agcgcgccgg tgaagcagaa atccaacccc gcgcaagaca gaacgttgta
     7561 ctcgagatgg gtatgcttct ttcgtcctta gggcgcagca atgtagcaat ccttcagaaa
     7621 cagcacctag aacagccttc tgatgctaac gggattttgt atctgaattt caacgaccat
     7681 gtacgtgaaa cagttcctcg tctagtgcaa cggttacaaa attctggttt cgaatttaca
     7741 caagctcaaa tagctaacgc ttcctcgtaa ttacccctta ttgttgatta caaccctgca
     7801 tcgcggggtt ttttatttgg gaataaaaac cctatgtcat ttaaataccg ggacagtccg
     7861 ctttattacc ggactgtgcg ggaggctttg caccttgaac agtccggcgg gtacgaccgg
     7921 gcgatgcagg tctgggccaa agcgaacgtc ggtttgattt ttgtctgatg cagaacatgc
     7981 gcgaaaagcg taaggaggtg gacgatgtct aatatcgata ataagggttg ggggttcccc
     8041 gctctgtcaa aaaaagcaca tttctttaat tcgggggaag tcatatcact atgcggaaaa
     8101 tggatgttca taggtatcag gattgatgaa tggcatgacc atcctgagaa ctgcgctatc
     8161 tgcatgaaga aacgcaaaaa gcaggaaggc gaaagctaat ggcctatgaa cgtgaaaacc
     8221 taatcgaaaa gcacctcgtc gctgaagtaa aaaaggctgg cggggttgcc tttaagttcg
     8281 tatcccccgg tcgccgctcg gtaccagatc gcattgttct gctacccggc ggtcgtatca
     8341 ttttcgttga atgtaaatcc cccggcaagc caccacggcc tgaccagttg cgcgaacacg
     8401 gacggttgcg cgcgctgggc tttaccgtag tggtgctgga tagcaaaaat ctggaggcca
     8461 ttattttaag ccgcaataaa ggataaaaga cttatgcttt aatccgtatt tttaacacgt
     8521 atttcgaaac actcttttaa tttagctatt tcctttttag aggacatgat tgtgaatctc
     8581 atccctttta aatcattaac aaattcaaat tttgcgctat ttgactcaca atcaccaaca
     8641 acaaaaccac taccaaatga attgagcaac actttagtat tactaccatc taccatataa
     8701 tattcgttat ttttaaatgc agtaaccttc ccgaatgtat agctcaatat caacaacgtt
     8761 atcaccccta aaggaaaagc tgactttagg atttttaaat cgatataata aaatacgttc
     8821 ttatactctg caagcctccc aaattttcta atgctctcca tatgatgttc gtgtctctta
     8881 ctatggattg gcatggacac cacattgtga taattcaaca aaagcatagc aaataataaa
     8941 atatcctgaa cactcatcac agaagtttcg gagaaataac caaaaggcca atgaaatcct
     9001 attatacaaa tattagccat tagcataaca actataaata caataaatgg aacataatat
     9061 ttccctttca aatcaaacat caacgccgca tataaggcgc cgagaaataa aaaactaaaa
     9121 aacaaaagcc acacagccga atacaatagc gtatttaagt ctattttaat aaaaaggtct
     9181 ggatagccat aatacgatgc aacacctttc tgaaaagaat atgccatcgc ataagctaac
     9241 gctgaaaaca tagttaataa agaaacttgc tttgtaaata ttttcgccag gggcataccc
     9301 gcctccgtta gttatatata tatatataga gagagagaga agtgaccata ttctaccata
     9361 ggtgtattaa tgaatagcat attccacccc agaatttatc aaaacctcat aatcaatcac
     9421 caaactaaca ttctgcgcgg caacatctgg gcgggtatgg gaatgggtaa aaccgtggca
     9481 acgctcactg cgctggaaga tctctttatg gcaggggcag aaacacgccc cgcactggtc
     9541 ctcgcgccgc tgcgcgtggc tgccagcaca tggccggatg aagcactgaa gtggggacat
     9601 ttgcgcaata tcgaggtgca gccaattatt ggtaatgcca aagcgcgctc tatggcgctg
     9661 acgaacagca acgcaagcgt gttcaccatc aactacgata accttgtctg gctgattgaa
     9721 gaattgggag aacgatggcc gttcggtact gtcattccag atgaaagcac ccggctaaaa
     9781 tccttccggc tgcgcggtgg tggtaagcgc gcggcggcgc tgggcaaagt ggcgcataag
     9841 tatgtccggc gctggataaa tctcaccggt acgccagcac cgaacggcct ggtagatttg
     9901 tggggacaat cgtggtttgt ggaccagggg caacgtctcg ggcgcactta cggcgcgttt
     9961 acctcacgct ggttcaactc gatacagttt ccggggcaga gctggaccaa actggagcct
    10021 tttgctcact cacagggtga aatacagcga gcgttagccg atgtaaccct ctcgctggat
    10081 gcggccgact ggttcgatat caaagacccc atccataacg taatccgcgt ggatatgcca
    10141 ccgaaggccc gtcagcagta tcgtgaaatg gaaaaggaaa tgttcctcga gctgaatggc
    10201 gaaggcatcg aagcaccgaa cgccgcggca aagacactga agtgtctgca aatcgccagt
    10261 ggcgcagtat acacagacga caccggaagt tggtcagaac tgcatgacat caaactgcag
    10321 gcgctggaca gcatactgac cgaagcagct ggcgcacctg tgctggttgc ttatcactgg
    10381 aaacacgatc ttgaacgctt gcttaaagca ttccctcgcg gtcgtcacct cgaccaggat
    10441 ccaaagacac tgcgcgactg gaattccgga aagattcctg ttctctttgc acacccagcc
    10501 agcgcgggcc acggcctgaa catgcaggac ggcggaaata tactggtatt tttctcacac
    10561 tggtgggacc tggagcaata tcagcaaatt attgaacgta tcggccccac caggcagata
    10621 caggccggac acaatcgtcc ggtatttatt caccacatta ttgctgccga cactatggat
    10681 gaaatggtga tggagcggcg caactcaaaa cgaacagtgc aggacatcct gctcgatgcc
    10741 atgaaaaaga gaggtatagc atgagcgaga aacccgacga tttactcacc ccggatgaag
    10801 tatgccaaaa gttaggtatt acacagaaaa cgctatgtga gtggaatatt aagcatcgtc
    10861 atcgggctat cctggcacca attcgtttca gtgcaaaagt agttcgttat gagcgccgaa
    10921 atatcgacgc ttttattcaa aaatgtcgca gccagtatta acctcgccgc cgtagcaatg
    10981 ccacctgcgc aagtatgctc cgctcgtgag cctcgaaagc ttcgcgcttc aacgcaatct
    11041 cttcctgtaa aatctcatca gaaaagtcgt aatgttctgc catcgggtca tctgacttgc
    11101 tggagtggtg aaggcaaagg aggctgattt cccttcggtc tgagcgggaa tagcctcttt
    11161 ccttcatcaa ggcaataaca ttgctcttaa ggaatttacg gcacatcgta ttaaatgcac
    11221 cgtctttccc tttaacagtc ccatcatgtt ttattccttt tacagccccg tccgggctgt
    11281 atgttttcac cagcttatcc agtgatcgtt ttgaaaatgg ctgcattgga tcacgtggct
    11341 gcaaaaatac ataatccctg ttgcactcag gaactgaatc acgccaggct ttctgctcgt
    11401 cgataatccg ccggatctca ggggttatcg gcaggcggaa agccttttgt gttttcatag
    11461 cccctcgcat gccgataacc ccttccggat aaacaatttc accagcctcc tcgtgaacgt
    11521 agtcccagcg caggttatgg acattaatcg gacgaacacc agtgatgatc atgaagcgaa
    11581 cagcattctt ctggtgtaca gaggtgcagg cagcaacatt gagccagagt cgggcgattg
    11641 attcaatatc ggtaaaaagc cgtgtggggg tgggtttctg tacgcgggag gaaacataat
    11701 catctggcag actggcagca acattgcgac cgttacaaag agtaggtgcg cagaacttcc
    11761 agaaccgacg gagctcggca aacaactcca gggcgttatt gttcgagcga gtggcgatcc
    11821 actcgtccag cacttccacc agccgattgt atgttacgtc gctgaacacc tcacgctcgc
    11881 cgaacgttgc tttaatccgg tcgatacgca ccccgtaggt tgtgaaactg tccgggctca
    11941 gcttctgccg ggcgactttg gctttgaggt catcccggta catttccagc gctgcatgta
    12001 cggactctgc ccgcaagcca ccgtcagcca tacctagcgc tttttcgcgc gccagctgga
    12061 tagcgagctc cggctactcg ccgagctttt tacccttgag gcccatcttt tttggaaact
    12121 cggcgtaaaa tgtaacctta ccggctttgc tgaaatcgat acggagatag ttctcttttt
    12181 cgtatttgga acggcgagcc acgccggaag cagcgaggat gattttggcg gcagcaacac
    12241 agattttcat gtgtgcgctg gtataggggg gtttacaggc gtcccatttt tcagatgcgg
    12301 ctaaaacatc gtcattattg gggctatccg gattatgtgt tacagtgcgc ggcattctca
    12361 atccttatct gcgtaggcgc agaaaacaag ctcacacata caagtctttt ctacgggaca
    12421 aaatgcaatg tgttgcggtt ttgtgttgct ggactgagtt tatcaaggtt aaatacactg
    12481 gatcaacata cagtaagtta atgacagtaa agcatacaaa ctcgatacaa cttactgatt
    12541 ttaaaatgat ttaacggtaa tccattgaaa tgtctttact aattactaaa cgctgtatta
    12601 attgtgatat gtgtgaaccc gaatgcccga atgaggcgat ttcaatggga gatcatatct
    12661 acgagattaa cagcgataag tgtaccgaat gcgtagggca ctacgagaca ccaacctgcc
    12721 agaaggtgtg cccgatcccc aatactattg tgaaagatcc ggcgcatgtc gagacagaag
    12781 aacagttgtg ggataaattt gtgctgatgc accacgcgga taaaatttaa tattctactc
    12841 tggaagtaga gtattaatta tattactggg aagccttaac gccattatat ttatttaatt
    12901 gatg
//
LOCUS       DBBGLQ010000123        12638 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.123, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000123 DBBGLQ010000000
VERSION     DBBGLQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.123"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..372
                     /locus_tag="ACIYSB_004491"
     CDS             <1..372
                     /locus_tag="ACIYSB_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late promoter activating protein"
                     /protein_id="HGC5431398.1"
                     /translation="EIADIMIRADCYLTVTEITTLAKEKYPHLHVSRVSVTNIIRHFV
                     RSSRAICELDDRVYPRKYWLHGLNGYQFKVRGRTPEYGSLLVKNCSRKSVEQARKEQR
                     ELVDMANKLWNAAVKKRGVAL"
     gene            369..1733
                     /locus_tag="ACIYSB_004492"
     CDS             369..1733
                     /locus_tag="ACIYSB_004492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA pacase A subunit"
                     /protein_id="HGC5431399.1"
                     /translation="MSLVNWEEHRARFIALREEKGITVKEYCEEHGLSFNTARKHLNM
                     KKNEVRSQVKTTKKSTKSPEKKRAGRATKKLEEGAEKTKFTEIDNSQGAGSFRSMPRK
                     KTSDHDNNTNNNNEIKESVRRSLREKMAKMIPAEDKCASKTEGSSAMIPGGAVQRATL
                     PTTSVARDMMKNGAEEHLRLAIQMAQERALQYQSIVDQEAERLQAEIDALGDQQPEGM
                     HPGQRLLGLISDAAYYMNDFISRLAAIYQSEQKLRQGDEKLRQGARQQAFREAEAREK
                     LELARLQAEQRGKEIEYRIGADARAARVIAAAIRMREREELDDIGVAEYIERQGVSVP
                     AILAARAAKAITLLEPPVSDINDVDDEQLDKEAKEFASLQANHPQWLAERRADVATIV
                     EDLGCGDYDHNGERKAGEFEANDEELDIDPSATAEIYGDYDASDAGYDAGDDDIAIEP
                     PEDD"
     gene            1787..3241
                     /locus_tag="ACIYSB_004493"
     CDS             1787..3241
                     /locus_tag="ACIYSB_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGC5431400.1"
                     /translation="MVIKYRYNFTQAVVDIFGMFPSHQQQEIIQSVQQKGSRTTVTSG
                     HGTGKSSLTAMLLLIYLILFPDARVIIVANKIGQVKTGVFKYVKQYWANAVKRHGWLQ
                     TYFVLSDTMFYERSRKGIWEVLCKGYRLGNEEALAGEHAAHLLLILDEASGISDKAIG
                     VMTGALTEEDNRMLMLSQPTRPSGYFYDSHHSQAKTPDNPKGIWTAIVLNSEESPFVT
                     PQFIRQKLLEYGGRDSIEYMVKVLGQFPREINGYLLGRDECDRAARRKVLLEKNWGWV
                     ATADVGNGRDKSVLNICKVSGHREKRRVVNFKVMEMPGTMDPLAFADFIYNECTPDKY
                     PNITIAVDADGFGSDTCAQLVRRGANPVRIRWGKPMFSNKDKERFVNQRAYANIMARD
                     AIKSGRMRIDSDPKTAEQASKIPFLLNEEGKMAMMRKEHMRQKLNIKSPDRWDTYCFT
                     MLVDYVPANEDIGAEMATFRNQVLADVEMPDLDI"
     gene            complement(3313..4350)
                     /locus_tag="ACIYSB_004494"
     CDS             complement(3313..4350)
                     /locus_tag="ACIYSB_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HGC5431401.1"
                     /translation="MSKKSIEKEYKRFLQTAARWKELVVANSVFHDTSYAGEEFRHVA
                     LTHDQNVLEEAEKCLTEWKAFVDLCRNADGKASNIVESVYSPIPFIIEDTNQSTHIVV
                     QSATTTRSFTRENLLKKYDAIIKKSLKNKIFSQIVGALEEERRFFASEPEGEVYRARK
                     DGYTDVVLTTNIEGSNALSRFRVGAHGALVFAKLPNTTVPVVNNVGERRSITIYSGVE
                     SIPCGLLGDFSLYRVRDLEKHQPSYVAKSYILRNIDIRNESLKNKSAKMLEEADPAIR
                     HIIERKIQTAREAMARLNKMDLELLDVMMTSGDDLTGIKLTDARKRYGKTIEERYGFT
                     FSQTQHAAKLW"
     gene            4736..5764
                     /locus_tag="ACIYSB_004495"
     CDS             4736..5764
                     /locus_tag="ACIYSB_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5431402.1"
                     /translation="MSELIISGSSGGFLRNIGKEYQEAAENFMRFMNDQGAYAPNTLR
                     DLRLVFHSWARWCHARQLAWFPISPEMAREYFLQLHDADLASTTIDKHYAMLNMLLSH
                     CGLPPLSDDKSVSLAMRRIRREAATEKGERTGQAIPLRWDDLKLLDVLLSRSERLVDL
                     RNRAFLFVAYNTLMRMSEISRIRVGDLDQTGDTVTLHISHTKTITTAAGLDKVLSRRT
                     TAVLNDWLDVSGLREHPDAVLFPPIHRSNKARLTTTPLTAPAMEKIFSDAWVLLNKRD
                     ATPNKGRYRTWTGHSARVGAAIDMAEKQVSMVEIMQEGTWKKPETLMRYLRRGGVSVG
                     ANSRLMDL"
     gene            5838..6182
                     /locus_tag="ACIYSB_004496"
     CDS             5838..6182
                     /locus_tag="ACIYSB_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431403.1"
                     /translation="MQSQITESRRFKVEGYTVTFAAGVKNSHAVVVFGIMTGLEAVPV
                     FTLEKNWRNLAEAESYVRKVTIIAAEKLLAHYQENYRTMVEKINLAFTRPTTGFIAPD
                     TNAGRYPARGRR"
     gene            6179..6655
                     /locus_tag="ACIYSB_004497"
     CDS             6179..6655
                     /locus_tag="ACIYSB_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431404.1"
                     /translation="MKTYYPSLNCYPSSYPELSGKLLALLAKAPTEWFKPVVINPHFL
                     DYGTVRTRNELKNLLAHGFIRHRAGKGYQLAIAPEEAVRLFAYRDSQTKARVIAQILH
                     NGSTYARQFGGDTSQFLRAVRSLEEQGVIESSCLPVPTVPHIKRRVYTFTQRAKKQ"
     gene            6652..7146
                     /gene="dut"
                     /locus_tag="ACIYSB_004498"
     CDS             6652..7146
                     /gene="dut"
                     /locus_tag="ACIYSB_004498"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGC5431405.1"
                     /translation="MIYVKVKRLHPAAKLPAYATSGSAAMDFEAVEIKPCVDSNGAIS
                     SSWWVYTGLAMEIPPGWCLKLYPRSGLGCKKHTRLANCVGIIDSDYRGEIMAKLITDP
                     GGEGVCLKPGMAVMQGIFERVEQVSLVEVEELNETERGAGGFGSTSEDHFEIKRTPLQ
                     YAKF"
     gene            7161..7811
                     /locus_tag="ACIYSB_004499"
     CDS             7161..7811
                     /locus_tag="ACIYSB_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HGC5431406.1"
                     /translation="MNYMATKELFKFLPKYKAHKNVSASKIKNVVIFGVTDAPLWGCI
                     ELVEPKGATVCVDAMFLQKHRPKTGGYLIVYENGYLFYAPEKPFEECYGLVNDASENG
                     TTVIITDDNGVMINITGSVTISAGGTTTQQEEIDLEAADFSDALMWLKEGKNVARREW
                     KGEKQFCWLVLAGSVNLHDIQVPYGDHFVLKDAQNMDSVWLPSVDDLLACDWFVVE"
     gene            7818..8585
                     /locus_tag="ACIYSB_004500"
     CDS             7818..8585
                     /locus_tag="ACIYSB_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431407.1"
                     /translation="MESTKTIQYRLRNGLLVAVNADMSLPFDTIERTIMTYLGFNEEL
                     NEEHGVAIWSDADSGVRRYITARGKDYSLEELFALAQSFECVALDLFNDPAIAQRLIR
                     ELGLSVTPIIFKNGSLTGTWRVERISNYLPYNRQLNGVISGVNQPVACENVNLVVAVL
                     ATACRVIGLAKQAFIHFPNGAEGSAEIIACDFEFTWMLREYLDQTVFRAEELDMYITS
                     TIPDDVRAEAIATARAKCRAAIAEQAKEEVKEVADGD"
     gene            8575..9669
                     /locus_tag="ACIYSB_004501"
     CDS             8575..9669
                     /locus_tag="ACIYSB_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431408.1"
                     /translation="MATKSNIYKDPRWLSLVEKYKDNWVLAAKELFDIDLSHQQQQIV
                     EAIQPNNAKATVTTPHGIGRPQVLAVISTLYTIMYPDSRTVIVYPKSNACKKGIVAYV
                     WQCWEALLKKQPFIIEYFKVGDSGLMFNEFWGMCFCNYRLNYEDSIAGHYADHLLFII
                     VDSAHISDRAYSIVWASMTSGDSRILLTSIPSPEEIGFFYDSHHGRALAEDNPSGVYK
                     AIKLSAEDSPFITQEYLDHFAERYGGRNSDDYRRMILGEFPGIREAVLESDMPKTMRF
                     SMPDGSEWTMPLRVIAKHHAQHHAKKHGVTTLEWLKSHTIPLFTADHNAIVEWAKTIP
                     WGNVAEYAHMLKPPKDRQEISWLTAEKIIE"
     gene            complement(9705..10085)
                     /locus_tag="ACIYSB_004502"
     CDS             complement(9705..10085)
                     /locus_tag="ACIYSB_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGC5431409.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGIQVYDSPVLVELAV
                     GAATGEIPVSSVAEKLRELYGSNI"
     gene            complement(10085..10306)
                     /locus_tag="ACIYSB_004503"
     CDS             complement(10085..10306)
                     /locus_tag="ACIYSB_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGC5431410.1"
                     /translation="MQSINFRTARGNLSELLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            complement(10379..10768)
                     /locus_tag="ACIYSB_004504"
     CDS             complement(10379..10768)
                     /locus_tag="ACIYSB_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGC5431411.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            complement(10856..11140)
                     /locus_tag="ACIYSB_004505"
     CDS             complement(10856..11140)
                     /locus_tag="ACIYSB_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin PHD"
                     /protein_id="HGC5431412.1"
                     /translation="MPTKAELQARIEILEKENASLKGMLARAERELSGKLLPEELPPA
                     DIPDRVSWWMKYFRAPWEAFWCYDHRRWCDELDSSFPYFAEGNSCPECRS"
     gene            complement(11124..11873)
                     /locus_tag="ACIYSB_004506"
     CDS             complement(11124..11873)
                     /locus_tag="ACIYSB_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431413.1"
                     /translation="MTRITKERISDIISRIEMYGHGAGYTADEVLELAKIALAPCKDG
                     KAMTLIDLLVKELSKGRGWPDGKDFCHLSITMPGSSRATVLFGTRHKNYQSSKNYSID
                     GIICDISDLDRSAFTSVVTREQYEAARIASQKVKSESKAYKLDFEQWLEQQRGKIDVD
                     CGCVSAETFMHWLRVAYEAGNHPDFPDSSQQAPRKSVKTTLERGYLEAALKIKPGHTL
                     GVIDAMLVHEMAKALLPLVADKHEADHANES"
     gene            complement(11870..12079)
                     /locus_tag="ACIYSB_004507"
     CDS             complement(11870..12079)
                     /locus_tag="ACIYSB_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431414.1"
                     /translation="MATFSKFAVIIILLIPLTVTAGEQQHCTKENEHPFIVIQCDDGT
                     VTVVNVRNDRVAVCRKGEPCKEIKL"
     gene            complement(12104..12397)
                     /locus_tag="ACIYSB_004508"
     CDS             complement(12104..12397)
                     /locus_tag="ACIYSB_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431415.1"
                     /translation="MWRGNNHGGSQMILTEYTFDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNLGNFKPTISLDDFPRGLSEREAMLKLAEWLQRLSIAIEDNWIQP"
     gene            complement(12421..>12638)
                     /locus_tag="ACIYSB_004509"
     CDS             complement(12421..>12638)
                     /locus_tag="ACIYSB_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGC5431416.1"
                     /translation="EEYRLCNDYWNKRIMFREYDRLIITKGYPKRDDFSRRIDVPYNG
                     YEIKTITHPHFGDKPVKVFAIKVKINS"
BASE COUNT         3383 a         2919 c         3042 g         3294 t
ORIGIN      
        1 gaaattgcgg atatcatgat tagggctgac tgctatctga cagtcaccga aatcaccact
       61 ctagctaaag aaaaatatcc tcaccttcat gtttcacgcg taagtgtaac caacatcatt
      121 cggcattttg tgcggtcaag ccgagctatt tgtgagcttg atgatcgtgt ttacccacgg
      181 aaatactggt tgcatggcct gaacggatat cagttcaagg tgcgcgggcg cacgcctgaa
      241 tacggtagct tactggttaa aaactgtagc aggaaatcag tcgaacaggc gcgaaaagag
      301 caacgagagc tggtggacat ggcgaataag ctgtggaatg ccgccgttaa aaagcgtggt
      361 gttgctttat gagtctggtt aattgggagg agcaccgcgc ccgctttata gcgttgcgtg
      421 aagaaaaagg gatcactgta aaggaatact gtgaggagca tggccttagc ttcaatacag
      481 ccagaaagca tctcaatatg aaaaaaaatg aggtgcggtc gcaggtaaag acaacaaaaa
      541 aaagcaccaa atcacctgaa aaaaaacggg cgggaagagc aacaaaaaag ctggaagagg
      601 gtgcagaaaa aacaaaattt acagaaattg acaattcgca gggtgcaggt tctttccgaa
      661 gtatgccaag aaagaaaact tcggatcatg acaataacac caataataac aatgagataa
      721 aagaatctgt caggagatca ttgcgcgaaa aaatggcgaa aatgatcccc gccgaagata
      781 aatgcgcttc aaaaaccgag ggatcttcag caatgatccc tggcggtgct gtgcagcgtg
      841 ccacgttgcc cactacgagt gtggctcgcg acatgatgaa gaacggcgca gaagagcacc
      901 tgcggttagc aattcaaatg gcgcaagaac gcgcacttca gtaccaaagc attgttgatc
      961 aggaggccga acgcctacag gcggagatag acgccctggg tgatcagcag ccagaaggaa
     1021 tgcaccctgg gcagcgactg ctcggtctaa tcagtgacgc ggcctactac atgaacgatt
     1081 ttatcagtag gttggcagcg atatatcagt ccgaacaaaa gctgcgacag ggtgatgaaa
     1141 aacttcgtca gggtgctcgc cagcaagcat tcagagaggc cgaggccaga gaaaaactcg
     1201 aattagcacg cctacaggcc gaacagcgtg gcaaagagat tgaatatcgc attggcgctg
     1261 acgcacgcgc agccagagtt atcgccgccg ctatacgtat gcgtgagcgc gaggagctgg
     1321 atgatatcgg tgttgctgaa tacatcgagc gtcagggtgt cagtgtcccg gctatcctcg
     1381 cggcgcgcgc ggcgaaagct atcactcttc tcgagcctcc agtttctgac atcaatgatg
     1441 ttgatgatga acagctggac aaagaagcga aggagtttgc atcgcttcag gctaaccatc
     1501 cgcaatggct ggcagagcgc cgggcggatg tggcaaccat tgttgaagat ctggggtgcg
     1561 gagattacga ccacaatggc gagcgtaagg ctggggaatt cgaggccaac gacgaagagt
     1621 tggatattga cccttcggct acggctgaaa tttacggtga ttatgatgca tcagacgcag
     1681 gatatgacgc cggagatgat gatatcgcga tagaaccgcc ggaggacgac taatggctgg
     1741 tgggcgcaag attaaatgcg tcaccagcga tccacgctgg cgcgatatgg taatcaaata
     1801 ccgctacaac tttacccagg ctgttgttga tattttcggg atgtttccct cccaccagca
     1861 gcaggagatt atccagtccg tacagcaaaa agggagcaga accactgtta cttctggtca
     1921 cggtacaggg aaatcgtcgt taacggctat gttgttgctg atttatttga ttctgttccc
     1981 cgatgcccgt gtcatcattg ttgctaacaa gataggccag gttaaaacag gcgtattcaa
     2041 atacgttaaa cagtattggg cgaatgcagt taagcgtcac gggtggttac agacctattt
     2101 tgtcctgtct gacaccatgt tttatgaacg ttcccgcaaa gggatctggg aagttctctg
     2161 caaaggttat cgactcggta acgaagaagc actggcgggg gagcacgcgg cacatttgct
     2221 tctaattctg gatgaagcat caggtatatc tgataaagcg atcggcgtaa tgactggcgc
     2281 tctgactgaa gaagataacc gaatgttgat gctatcccag ccaacaaggc caagtggtta
     2341 tttctacgat tcgcaccatt ctcaagctaa aaccccagac aacccgaaag ggatctggac
     2401 agcaattgtt cttaactcag aagaatcacc gttcgtcaca ccacaattca tcaggcagaa
     2461 actcctggaa tatggcgggc gcgattctat tgagtacatg gtaaaggtgc ttggtcagtt
     2521 ccctagggag attaacggct atttgcttgg tcgtgatgaa tgcgatcgcg ctgcgcggcg
     2581 caaggtgttg ttggagaaaa actggggatg ggtggctact gctgacgttg gtaacgggcg
     2641 agacaagtca gttcttaata tctgtaaagt gtctggtcac cgggaaaagc ggcgtgttgt
     2701 taactttaag gtaatggaaa tgccagggac tatggacccg ctggcttttg ctgactttat
     2761 atacaacgaa tgcacgccgg ataaataccc gaatattaca atcgcggttg acgcagacgg
     2821 ttttggttca gatacctgtg cgcagctggt gcgccgtggg gctaatccgg tacggattcg
     2881 gtgggggaaa cccatgtttt cgaacaaaga caaggagcgt tttgttaacc agcgtgccta
     2941 cgcaaatatt atggctcgtg atgccataaa gtcaggtcgt atgcgaatag acagcgaccc
     3001 taaaacggca gagcaagcgt caaaaatccc ctttttactt aatgaagaag ggaagatggc
     3061 gatgatgcgc aaggagcaca tgcggcaaaa gctgaacatc aaatcacctg accgatggga
     3121 tacctactgc tttacaatgc tggtggacta tgttcctgct aatgaagata tcggcgctga
     3181 aatggctacc ttccgcaatc aggttcttgc agatgtagaa atgccagatc tggatatttg
     3241 acatcctcca cgccctgaag gacggggttt tacggcgcac cggataaagc cccggcaatg
     3301 cggggcttgt gattaccaga gtttagcggc gtgctgtgtt tgggagaaag tgaacccgta
     3361 acgttcttct atggttttac cgtatctttt ccgcgcgtct gtcagtttta tgcctgtcag
     3421 gtcatctccg gatgtcatca ttacgtctaa caactccaga tccattttat tcagtctcgc
     3481 catcgcttcg cgtgctgtct gtattttacg ctcaatgata tggcgaatag ccggatcggc
     3541 ttcctcaagc attttggctg atttgttctt taagctctca ttgcggatgt cgatgtttcg
     3601 caagatgtat gacttcgcaa catagcttgg ttggtgtttc tccagatcgc gaacgcgata
     3661 gagactaaag tcaccaagca ggccgcaagg tatcgactca accccagaat aaatagtaat
     3721 gctgcgacgt tcgccgacat tattaacaac gggcactgtg gtgttcggaa gttttgcgaa
     3781 cactaaggct ccgtgtgcgc ctaccctgaa tcgtgacaga gcattgctgc cttcaatgtt
     3841 tgttgtaagc actacatctg tgtatccatc tttgcgcgcc cggtaaactt ccccttcagg
     3901 ttctgatgcg aaaaaacggc gctcttcttc aagtgcacca acaatctgcg aaaatatctt
     3961 gtttttcagg cttttcttta taattgcatc atattttttg agcagatttt cgcgcgtaaa
     4021 tgagcgggtg gtggtcgcgc tttgcacaac aatatgcgtg ctttgatttg tgtcctcgat
     4081 aatgaatggg atcggagaat atacagactc aacaatgtta gacgctttgc cgtcagcgtt
     4141 acggcacaag tcaacaaagg ctttccattc tgtgaggcac ttttcagctt cttctagcac
     4201 attttgatca tgtgttagtg caacatggcg gaattcctcg ccggcataac tggtatcatg
     4261 gaaaacagag tttgcgacca ccagctcttt ccaccgtgca gcggtttgca ggaaccgttt
     4321 gtactctttc tcgatcgact tcttactcat tattcctact cgatttagtt ttctaacata
     4381 aaattactat gacaaatcta tgccgaataa cagggtgagt aaaggatcaa gatgtgtttt
     4441 tttatcatca aatttagaaa attccgctta aaaactgcct ggcataactt aaagcccaca
     4501 ccagcaatgc attgcccatc tgcgcggaaa ggaaaaaatt tcctaaattt ctaactttag
     4561 ataattggca ttatttaaag ttaggctata atcatgtgta gatgaacagt gttgacatca
     4621 ctgaaaatac gtgcttcgaa tcaaaggctt gtattttgaa aattaactta tagctaccct
     4681 gtcattttta tatttttcgc gtagcgtcat ctgtatatag aggtcaaata gtttcatgag
     4741 tgaattaatt atctctggca gttccggtgg ttttctgcgc aacattggca aagagtacca
     4801 ggaggccgca gaaaacttta tgcggttcat gaatgaccag ggggcttacg cgccgaatac
     4861 tttgcgcgat ctccggttag tgtttcactc ttgggcgaga tggtgtcacg ctcgccagct
     4921 cgcctggttc ccgatctcac cagagatggc ccgcgagtat tttcttcagc tgcatgatgc
     4981 tgatctggct tcgaccacca ttgataagca ctacgccatg ctgaacatgc tgctttcgca
     5041 ttgtggcctt ccaccacttt cggatgataa aagtgtttct ctggctatgc ggcgcatccg
     5101 gcgcgaagcg gcaactgaaa aaggcgaacg aacaggccag gctataccgc tgcggtggga
     5161 cgatctgaaa ctgctcgacg tcctgctgtc caggtcagaa cggctggtgg acctgcgcaa
     5221 ccgggctttt ctctttgttg catacaatac gctgatgcgt atgtcggaaa tatcgcgtat
     5281 tcgtgtagga gatctggacc aaacaggtga cactgtcacg ctacatattt cacacacgaa
     5341 gacaataacg accgccgccg ggcttgataa ggtgctttcc cgtcgcacta ccgctgtgct
     5401 gaatgactgg ctggatgttt cagggcttcg cgaacatcct gacgcagtgc tgttcccgcc
     5461 gatacaccgt agcaataagg ccaggctcac gacaacgccc cttactgcac ctgcaatgga
     5521 gaaaatattc agcgacgcct gggtgttgct gaataaaaga gatgctacgc caaacaaagg
     5581 aagataccgg acgtggaccg ggcatagtgc acgcgtcggg gccgctattg atatggctga
     5641 aaagcaggtg tctatggtgg agatcatgca ggaaggaacc tggaagaaac cggaaacact
     5701 tatgcggtat cttcgccgtg gcggcgtgtc tgtgggggct aatagccgcc tgatggattt
     5761 ataaattaaa tttctaacac atctttttag attaacgcct ttgctgtaaa gtaccctcgc
     5821 ctatttagga gggtgctttg cagagtcaaa tcacggaatc aagacgcttc aaggtggaag
     5881 gttacaccgt aaccttcgcc gctggtgtta agaacagtca tgctgtcgtt gtgttcggca
     5941 tcatgaccgg gcttgaggcc gtccctgtgt ttaccctcga aaaaaactgg cgaaatctgg
     6001 cggaagcaga aagctacgtc cggaaagtaa ccatcattgc cgccgaaaaa ctcctggccc
     6061 actatcagga aaattatcgc actatggtcg agaagattaa tctcgctttc actcgcccca
     6121 ctaccggctt tattgcaccg gatactaatg caggtcgata tcctgcaagg gggcgtcggt
     6181 gaaaacttat tacccatctc tgaattgtta cccgtcttcc tatccagaat tatccgggaa
     6241 gctgttagct ctattagcta aagcacctac ggaatggttt aagccagtcg ttatcaatcc
     6301 acacttcctg gattacggaa ctgttcgcac tcgtaacgaa ctcaaaaacc tactggcgca
     6361 tggattcatt cgacaccgtg ccgggaaagg ctatcagtta gcgattgcgc cagaagaggc
     6421 cgtgcggcta ttcgcatatc gtgacagcca gacaaaagct cgggtaatag ctcaaatact
     6481 tcataacggc agcacctatg cccgccagtt tggcggcgac acgtcgcagt tcttgcgggc
     6541 ggtgcgatcg ctggaggagc aaggagttat tgaaagttcc tgtcttcctg ttcctaccgt
     6601 tccacatatc aaacgtcggg tttacacctt cacgcagaga gctaaaaaac aatgatttac
     6661 gtaaaagtga aacgcctaca tccggcagca aaacttccag cttatgccac ctccggatcg
     6721 gcggcgatgg attttgaggc tgttgaaata aaaccatgcg tcgatagcaa tggtgcaatt
     6781 tcctcaagct ggtgggtgta caccggactg gctatggaga tcccgcccgg ctggtgtctg
     6841 aaactctatc cgcgctccgg attgggctgc aaaaaacata ctcgcctggc taactgcgta
     6901 ggaattatcg actcagatta tcgcggtgaa atcatggcaa aactgattac tgatcccggc
     6961 ggggaaggcg tttgtctcaa gcctggaatg gctgtcatgc aaggcatttt tgagcgtgtt
     7021 gaacaagtct cactagtcga ggttgaggaa ctcaacgaaa cggaacgtgg tgctggtggc
     7081 tttggtagca catcggaaga ccactttgaa atcaagcgca caccgcttca gtacgcaaag
     7141 ttttaagcag aggaaaggct atgaattaca tggcaacgaa agagcttttt aagttccttc
     7201 ccaaatacaa agctcacaaa aacgttagtg catcgaaaat caaaaatgtt gttattttcg
     7261 gagtaactga tgccccactt tggggctgca ttgaactcgt tgaaccaaaa ggggcaacag
     7321 tttgtgttga cgcaatgttt ttacaaaaac accgaccaaa aactggcggg tatctgatcg
     7381 tttatgaaaa cggttatctg ttctatgccc cggagaaacc atttgaggaa tgctatggcc
     7441 tggttaacga cgcctccgaa aatggaacca cggtcatcat tacggatgac aacggcgtga
     7501 tgattaatat taccgggagt gtaactattt ctgctggtgg taccactacc cagcaagagg
     7561 aaatcgacct tgaagcagcc gacttttctg atgcgctaat gtggctgaaa gaaggaaaaa
     7621 atgtagctcg acgcgagtgg aaaggcgaaa agcaattctg ctggttagtt ctcgctggga
     7681 gcgtcaattt acacgacatt caggttcctt atggtgacca tttcgtattg aaagatgcac
     7741 aaaatatgga cagcgtttgg ttgccgtctg ttgatgattt actggcttgt gactggtttg
     7801 tagtggagtg atttgacatg gaaagcacca aaacaattca ataccgcttg cgtaatggcc
     7861 tgctggtggc tgttaacgcc gacatgtcac tgccgtttga cacgattgaa cgcactatca
     7921 tgacgtatct cggctttaat gaagagctga atgaagagca tggcgtagct atatggagtg
     7981 atgcagacag cggcgttcgt cgttacatca ccgccagggg caaagactac tctctggaag
     8041 aactgtttgc ccttgcacag tctttcgaat gtgtagcatt agacctgttt aatgatcctg
     8101 ctatagcgca acgacttata cgcgagcttg ggctatccgt tacaccaatt atctttaaaa
     8161 atggcagcct gactggcaca tggcgcgtag aacgcatctc aaactacctt ccatataacc
     8221 gacagttaaa tggagtaatc tccggtgtta atcagcccgt agcgtgtgaa aatgtaaacc
     8281 tggttgtcgc tgttttggca accgcatgtc gtgttatcgg tttggctaaa caggcattta
     8341 ttcatttccc aaatggtgca gagggtagcg cagagattat cgcgtgtgat ttcgaattta
     8401 cctggatgct acgcgaatat ctggaccaaa cagtgttccg cgccgaagaa ctggatatgt
     8461 atatcacgtc aacgattcct gatgatgtgc gtgctgaagc aatcgctaca gcaagggcca
     8521 aatgccgtgc ggcgattgca gagcaggcca aagaagaagt taaggaagtg gcagatggcg
     8581 actaagagca atatttataa agatccgcgc tggctgtcgc tggtggagaa atataaagac
     8641 aattgggttc tggcagcaaa agaattgttt gatattgatt taagccacca gcaacaacaa
     8701 attgttgaag ctatacagcc caataatgca aaagctactg tcacaactcc gcatgggatc
     8761 ggacgaccgc aagtgcttgc tgttattagt accctttata cgattatgta tcctgattcc
     8821 cggacagtaa ttgtttatcc aaaatcaaat gcttgcaaaa aaggaattgt tgcatatgtg
     8881 tggcaatgtt gggaggcatt actgaaaaaa caacctttta tcattgagta ttttaaggtc
     8941 ggtgatagcg gtctgatgtt caatgaattt tggggaatgt gcttttgtaa ctaccgcctt
     9001 aactatgaag atagcattgc aggtcactac gccgaccatc tgttattcat tattgtggat
     9061 tccgcacata taagcgatcg ggcatacagt attgtgtggg caagcatgac tagtggtgat
     9121 tcacgcatat tgctcacttc aattccgtca ccagaggaaa taggattttt ctatgattct
     9181 catcatgggc gtgctttagc tgaagataat ccgagtggtg tctataaagc cattaagctg
     9241 agtgctgagg attcaccatt tattacccaa gaatatcttg atcattttgc cgaaaggtat
     9301 ggcgggcgaa atagtgatga ctaccgtcgt atgattcttg gtgagttccc tggtattagg
     9361 gaggctgttc ttgaatcaga tatgccgaaa acaatgcgtt tttcaatgcc tgatggcagt
     9421 gaatggacaa tgccattacg agtgatagcc aagcatcatg cacagcacca cgccaaaaaa
     9481 catggcgtta caacattaga gtggttgaaa tcgcacacca ttccactctt cactgcagat
     9541 cacaatgcta tcgttgagtg ggcaaaaaca attccgtggg ggaatgtcgc tgaatacgcc
     9601 cacatgctaa aaccgccgaa agacaggcaa gaaattagct ggctgacagc agaaaaaatc
     9661 attgagtgat agtaggaagc ccgcttggtt gagcgggctt cttttcaaat attggaacca
     9721 tatagttcac gtagtttttc cgctactgaa gatacgggga tttcaccagt tgcagccccc
     9781 accgcaagtt ccaccagcac gggagaatca tatacctgta taccgttacg gcgtagaaat
     9841 agcagcgcac tgtttagcgc ggtacgctta ttggcatcat tgaatatatg ccctctcgca
     9901 gtagccacca ggtaggttgc ggagacttcg aaaaggtcgg tgatctcttc gtaggcaact
     9961 ctggcctgaa ctctcccgat aatggcctct gccctacccg gatctgacat tcccggcagg
    10021 ccgccgtagc ggcttatatt cgcatcatga agcgcaataa gttcttccgg tgatatatgc
    10081 ctcattatcg gttaaccagt tccttgttgg tggagtccag ggtgtcaaac agggatgcaa
    10141 attcagcatc cagcgccgcc tttttgtagg cttcgaaagt agccttgctg acaattactg
    10201 ctggttcacg gcctctgcgg gtgatttcaa cctcttcccc ggcttcaaca ttgttgagca
    10261 attcagaaag gttgccgcgc gcggtacgga agttaatgga ctgcataaac acctcgtgta
    10321 ctcgttatgt gtacacaatc ataaacttca caggcataaa gcaccagcac tttgcagctt
    10381 aaatgaccgg acaatcatca aactccccac ttcgggcatc attgatgaca tgagtgatca
    10441 caccaaaaac agcattacta cccgtgtaac catcgtcatc tactggtaac gcctctttct
    10501 tcccggtgct taaatcctcc aggtgctggc gcggatactt tcggtatctc tttatgcgat
    10561 attcaccctc catagcgcac acaagcagcg aaccatcaac tggagtaagc gaagaatcaa
    10621 ccaccagcaa agcaccctgc aatattccct cacggtgatg gctgtctgcc gcccgcatga
    10681 agtatgtcgc tgatggatgc ctgattagtt gctgatcaag agaaattctg ctttcaacat
    10741 agtccgccgc aggagaaggg aagcccatag cgttttcacc tcaataatac tgttcattta
    10801 tacagtatac attaaagaga cacctttggt gcaaacgcgt tatcgccggt catcattaac
    10861 tcctacattc agggcaagag ttcccttccg caaaataggg gaaactgcta tcaagctcat
    10921 cacaccatct gcgatggtcg tagcaccaaa acgcctccca cggtgcacgg aaatacttca
    10981 tccaccagga cactcgatca ggtatatctg ctggtggcag ctcttctggc aataatttgc
    11041 ctgataattc cctttccgcc cgcgccagca ttccttttag actcgcattc tctttttcaa
    11101 gaatctctat gcgcgcctgt aactcagctt tcgttggcat ggtccgcctc atgtttatca
    11161 gccaccagcg gcaataaagc cttcgccatt tcatgaacca acatggcatc aatgacgcct
    11221 agtgtatggc ccggcttaat ctttaatgcg gcctcaagat agcctctttc cagagtggtt
    11281 tttacgcttt tcctgggcgc ttgttgggaa ctatccggaa aatccggatg gttcccagcc
    11341 tcataagcta cccgcaacca gtgcatgaat gtttccgcag acacacaacc gcagtccaca
    11401 tcgattttcc cgcgttgctg ttccagccat tgctcaaaat ctaacttgta agccttactt
    11461 tcagacttaa ctttctgaga cgctatgcga gctgcttcat actgttcacg agtgacaacc
    11521 gatgtaaacg cactgcgatc aaggtcactg atatcgcaaa taatgccatc aatggaataa
    11581 ttctttgatg attggtaatt tttatggcgc gtaccaaaaa gaaccgtagc gcgactacta
    11641 cctggcatcg taatggatag atggcaaaaa tcttttccat caggccatcc acgcccctta
    11701 gataattcct tcactaacaa gtcaatcagt gtcatagctt taccatcctt gcaaggcgct
    11761 aaagctattt tggcaagctc caatacttca tctgctgtgt atccagcacc gtgaccatac
    11821 atttcgatac gggaaataat atctgaaata cgctctttag tgattcttgt catagtttta
    11881 tttccttgca tggttcgcct ttacggcaaa cagctacacg gtcatttctt acattaacca
    11941 cagtcaccgt tccgtcatcg cattgaataa cgatgaaagg gtgttcattc tcttttgtgc
    12001 aatgttgctg ttcccctgct gtaacagtga ggggaattaa taaaataata attactgcga
    12061 atttactaaa tgttgccata gcaatctttc tttctgtttt tcattatggt tgaatccagt
    12121 tatcttcaat agcaatgctc aatctttgta accattctgc taatttcagc attgcttctc
    12181 tttcgcttaa accacgagga aaatcatcaa gcgatattgt tggcttgaag tttcccaaat
    12241 tatccatttc aacggtcaga ttttgctcca gaacggtatt tcttacgcgg ctattgtgcc
    12301 gaagcaaata tactgaacgt gatttattgg ttttgtggtc gaacgtatat tcggtaagta
    12361 tcatctgact tccgccatga ttattaccgc gccacataat tactccgtgt taattgaaat
    12421 ttagctatta atcttcactt ttatcgcgaa cacctttacc ggtttatcac cgaagtgtgg
    12481 atgtgtgatt gtttttattt catatccgtt atacgggacg tcaattctgc gactgaagtc
    12541 gtcgcgcttc ggatatccct ttgtgataat caggcggtca tattcccgga acatgattcg
    12601 cttattccag tagtcattac acaggcgata ctcttccg
//
LOCUS       DBBGLQ010000124        12313 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.124, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000124 DBBGLQ010000000
VERSION     DBBGLQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.124"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..1534
                     /locus_tag="ACIYSB_004510"
     CDS             <1..1534
                     /locus_tag="ACIYSB_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGC5431417.1"
                     /translation="LGSNSDGMTSAADSISLSAELPGERLQKYNILETMAKSPTISTA
                     LNIHIAHALAPSKKTGQAFILSPKDGSDAEAVSRCEELTADLGAMINDGLPSWAMIMA
                     IFGVSYVRPYAEQGRGITGIESSYYTLPHFVQEFYRGSQLVGFSGDYILDTHSLRRVI
                     TEPWNLVSMKNPYWTPQHKVIPVSYGTKGYSLLSEQADKPLMETQNYGTSFLEYSYEP
                     YLNLCAALAALKSTRNNAAKIDRLIALTTNTLDPVNAANYTRGVSQALKRHSDLVAQR
                     SINANAIPTVLNHLIPVMGDGKNGITIDTQSIPADISGIEDVMFHLRQLAASLGIDAT
                     MLGWADQMSGGLGEGGWQQTAIQAALRANWIRQAAQRTIYRLHDIHLAYKYGKVYTEK
                     NRPYDVQFNSMNTAIQEEENRELDARANFVAVISQIMDQIQNNPKLAGSDAFMRYLFT
                     EQLHIDDDTLNTMIKEFKANESEQNNEHGMYESAPLSSGDDPENWTPEQLINFAKFVM
                     SN"
     gene            1548..2546
                     /locus_tag="ACIYSB_004511"
     CDS             1548..2546
                     /locus_tag="ACIYSB_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HGC5431418.1"
                     /translation="MQSLNTVTDRFSLVEKIRKHTPQNNRNYVIQSVRDTFNSPETKE
                     RIALGEMYGYYGHGRRAMHYNKTKSLNLPEVSVVMVDGKPVVLENVPSNRTIDISIDD
                     NGIVTHTQEILDTDTGRIVQGMINSGAGGWSWATSGPDSSVSLVKSFHGFDYVTVPNY
                     ISLDKKSLMLESAEERDAAIHAALIEQGFTDNAATDLVHHFSAMKNQQAMLESAHREA
                     LESELMLLEAENMQLRDKLRNQAAMMESQGEDAKKCRRILRDAIQEMPVFISAEQRRA
                     LCRMQSEDDARIVAAMLESLGANATYGLPISGKKEAELSPKENKNTTPLLFVSRRA"
     gene            2747..3709
                     /locus_tag="ACIYSB_004512"
     CDS             2747..3709
                     /locus_tag="ACIYSB_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic replication protein"
                     /protein_id="HGC5431419.1"
                     /translation="MSILNSVSVTFNTSEYKNNTEYHKKIKGHQLSFFARHCGFLSPS
                     HSAIIAEFANLAGATDEYMIRRSYADMSEITGRSISTVRRAFAEATKCGMLVKQHQVA
                     NNNAQVCNVYRFTTQFLHFIHVAMEIGGKQGIKFANATKLVKQLISNVRYFFETGNPL
                     FKLNKSPHVQNEQPIENKSHSIAKRRERSCAVQPKASPADSSQADNGLLDKKPVIREE
                     HTNHCLAAAKARAAKRRSDEGHAKRQALYRTAEKLAKKFAWIRSAATAVNKPKQSSAL
                     DFSMDYSGSQGCVTIGEAFDLMKQRGYRSEFDREDWSIPAGFRG"
     gene            4123..4347
                     /locus_tag="ACIYSB_004513"
     CDS             4123..4347
                     /locus_tag="ACIYSB_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGC5431420.1"
                     /translation="MVNANPCSRPEFIWRFHSLQKHCDHFAIAATEKEARSLIPDQHG
                     VLTGRFSTSPQPNTNPWYRNITNPGIAEAH"
     gene            4347..5054
                     /locus_tag="ACIYSB_004514"
     CDS             4347..5054
                     /locus_tag="ACIYSB_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGC5431421.1"
                     /translation="MDICILEKPSMTSIEIAELVGKRHDNVKRTIETLAESGVIVRPQ
                     IEDEPKIDVMGRSRLIQVFRFSDEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQVRQ
                     PLTEIEMIAAMAANAVQQQKRLHVVESKVSQVVETVEQIKKGNIPEGYIGYRQLAAKC
                     GLTEAKCRNLVNAYRIPTDTHEFLTPEGVLSRRSIVALSPFMNAFNRMMSEAEHRGKR
                     WYHPKMGQFQVIGWGGE"
     gene            5054..5251
                     /locus_tag="ACIYSB_004515"
     CDS             5054..5251
                     /locus_tag="ACIYSB_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431422.1"
                     /translation="MDIMQAVKKIREGQAEVCRRKAWSVGVWAWPDNYSTTGWFCING
                     LQIREVDLSTADIVGNDWIVT"
     gene            complement(5284..5769)
                     /locus_tag="ACIYSB_004516"
     CDS             complement(5284..5769)
                     /locus_tag="ACIYSB_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HGC5431423.1"
                     /translation="MANIEPRWLIEARKHIGLTEIKGAKHNPEIVQFWRDIKRGGIKD
                     DETPWCAAFVGAMLERVGIRSTRFESAKSYLDWGEKLDTPAYGCIVVFTRVGGGHVGF
                     VVGRRANGDLLVLGGNQGDAVNIRAFPTSRVSGYRWPAGEPRNTALLPVGDAATSTNE
                     A"
     gene            5920..>12313
                     /locus_tag="ACIYSB_004517"
     CDS             5920..>12313
                     /locus_tag="ACIYSB_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HGC5431424.1"
                     /translation="MDKLLHSISEASSLGELIEIVSRLHKLRAVATFGVKKVDGVSIQ
                     KTRRAANNAAVELLNSLPPGFDGAKLTDEQRQILAGYTGEGGLTDGEGSQYEYYTPQF
                     MAEGIWDLFADYGIDGGHVLEPSAGTGIFQETKRQGMIMTSAELSPISGRINQLLHPE
                     DDVNIGAFEALAAKDAMYDHAVGNVPFGEGRSGVAGLDPAYANEKNVGNYFVLRTIDK
                     VKPGGLIVLVVPNGMTDGTKYKKLRDKVSRKAEFLGAHRMPSGTFSESGTDTVVDVWV
                     LRKHPETFLEMIPDTDDATLKSANVLWDTFLKGKWFTTEGKRFVYGDMERTSFRNTLV
                     VKKDGRVSNESMKNALSRRFESRINWDLLGVTTQAWQGAKVGDKRLVGGIWHEFDGLK
                     WVKDATTKSSALDVNRYGVTTFGDLQIAFQSTNGILALSWDQISAIASDYPSVISDEV
                     AAMIRFAGKQREKDRERVMRGALIGQLINKALDKRNLGENVDDELADAARLAEAEIAK
                     YGPPHAIKLNGIAEAGAKNWMTFTGNVKQDGSASDLLAGRLDVTDGAAGIDFTRPEQV
                     VTHLFSDVALNPITLDDFREAFAGELPEDEDAALEYLAKFDDIAIDGYGCLLPMDRAT
                     SGDIATKTALLVGWRDASTGEQKANFERQLAKIEEKRIFTPLNKVTVNLNARWLDRRL
                     IKEFLAEQGYDEFKYTEPDLKVEDGILVSPDDYEGKDGVFTGYQLRTVNGKNGNEFKK
                     ANNKDGFLNQLENYLNGIKPRGQNANEYLDKIAQLEISFNDWLRTHPQADQIARDYND
                     AFNGFIPFTHSDAPLGLEGISGKRIPLTYQNEEVRRLSEDGRGIMGFGTGLGKTTTAL
                     ALEAYNFETGRTKRTCIVVPKAVYQNWYHEAQSFYSADAFANMMFIGLDEVRDDSGNI
                     LTAPVLDENGEPRLDSNGQPLTRNVVKESASAVIVQRMNMIPSSNWRTVIMTKEQFAS
                     IPLREETIEENSQQAVFNAVEMGRLDLASGKHRDAQKKNKIKDQAANTGTTKKQNIPY
                     FEDMNFDSVIADEGHNYRNSFSAGREAGQLAYLPNPAVSKMARDMAVKAAYMMKRNNG
                     RGVVMLTATPLVNSPIDAFNMLSTVIPQEEWMRMGIVTPDDFVRVFGKTATVQVQKIS
                     GEVEEKQGLVGFQNLDGLRGIFHRWTTLKTAADVGATVKIPDIVENTLQIPMTGEQEA
                     IYEELRKRAQELSSKDALTVDENGHITNEKPDDFIFSIIRDMDKVAIDPDLYASAITF
                     QFPVELAEKVKAIADALPKVAGGKVADDADEAEEDGAGGLVSTRTSKVVKTTFSEHPS
                     HVEIRAGIELEAEILKAIAAAGIDMQQVSHPIPPKYAALIENLREGLKNGKQIVFIDE
                     KAQHQKLRRIIASALQMPEQEIGIINATTVSQAGGVKLKKVNKPTEPTPNKNGEYKEG
                     AWETYYSKLAQYEDYLSAKNDAGLEGMEGIAADYNEGRTRIIICNKKAEVGINLHIGT
                     TDIHHLTLPWTPASIDQRNGRGARVGSPQEKVNVHYYCGKGTFDDFRLDTLKRKKDWI
                     NMVMTSDMSEIANGDADDADERAIMLAANPEERRAIMSRQAQEREERLKLKALREANN
                     ALDNYLKAANAAGKDIGMMETELKSAMEQVEHYQRNLDDLIKAGTNRNGQKYVLEQLR
                     DYRKRVRELRFAITRAKDAGTVMKRSRGDLERAIKAGVLELDADVLQNPQEYVRTEKN
                     ILLHKGSHYRAIIDENHNTTAIVRVTAIDAERGGCTCRISWLDVASRYSRSPGTALFL
                     PFNLIIESVTFEEGVAESRETAARGVQCHQLSTTLTRDQFYDAIRNGVMNVTAGKPGR
                     VWGGGSAIKYWAYRTDDGQIALSSTYDGVIGQKIGRASDIAPERWIYPDGNDEALKRE
                     VAKIQASDNPLNVVDGEGFFQALYGYDYIQGMQAWGAQATMDDVSQEFENWMAGRGLS
                     GRSLNKVTSEDILSLFRKPSGEGLESFWKTVVAYRLFQGRMKAFSNTDDIERMFNQVK
                     AAKITAKIEKTRAALVAWRDALFEQYKSLSDADGWSALEQLANNGVDGAKLAIVQADN
                     PLDSPAAQWIKVGAVINAFDEKHISKDDFADAVSVNRLVEMAVRRARALKDGDYVNE"
BASE COUNT         3329 a         2801 c         3283 g         2900 t
ORIGIN      
        1 actggggagc aacagcgatg gcatgacgag tgcagcagat tcaatttctt tatccgctga
       61 actgcccggc gagcgcctgc aaaagtacaa catcctggaa acgatggcta aaagcccgac
      121 tatctcgacg gctttaaata ttcacattgc acatgcgctt gcgccttcaa aaaaaaccgg
      181 acaagcattc atcctttcgc caaaggatgg ttccgatgct gaggcagtaa gtagatgtga
      241 agagttaact gctgatttgg gggcgatgat taatgacgga cttccttcat gggccatgat
      301 tatggcgatc tttggggtgt cttatgtccg cccttatgct gaacagggca gggggataac
      361 aggcattgag tccagttatt acactctgcc acacttcgtc caggagtttt atcgtggtag
      421 ccagctggta ggtttcagtg gtgattacat cctggatacg catagtttgc gcagagtaat
      481 aaccgaaccg tggaatctgg tttccatgaa aaatccctat tggacgccgc agcataaagt
      541 tattccggtt agctacggca caaaagggta ctcactgtta agtgagcagg ctgataagcc
      601 gttaatggag acgcagaatt acggaacttc attccttgaa tacagctatg aaccttacct
      661 gaatttatgt gctgcgctcg ccgcgctgaa atcaacgcga aataatgcag ccaaaattga
      721 ccgcctgatt gctcttacga cgaatacact ggacccggtt aatgcggcga actatacgcg
      781 tggcgtcagt caggcattga agcgtcatag tgacctggtg gcgcaaaggt cgattaatgc
      841 caacgctatt ccgacggtgc taaaccacct gatacctgtt atgggggatg ggaagaatgg
      901 catcaccatt gatacgcaat ccatacccgc agatatcagt ggtatcgaag acgttatgtt
      961 tcacctccgg cagcttgctg caagcctagg cattgacgca acaatgcttg gctgggctga
     1021 tcagatgagt ggtggacttg gggaaggcgg ttggcagcaa acagctatcc aggccgcatt
     1081 acgtgcgaac tggatcagac aggccgccca gcgaactatt tatcggcttc atgatattca
     1141 cctcgcttat aaatacggga aggtttacac cgaaaaaaac cgtccttatg acgtgcaatt
     1201 caactcaatg aacactgcta tccaggaaga ggaaaaccgg gaactggatg cacgcgccaa
     1261 ctttgttgct gtcatctccc aaattatgga ccagatccag aacaacccga aactggctgg
     1321 tagtgacgcg tttatgcgtt atctgttcac cgagcagttg cacatcgacg atgacactct
     1381 caacaccatg atcaaagagt ttaaggcgaa tgaatcagag caaaacaacg agcatggtat
     1441 gtacgaatcc gcgccgttgt cgtccggtga cgatccggaa aactggacgc cagaacagtt
     1501 aataaacttt gcaaaatttg tgatgagcaa ttaaccggag ggaaataatg caatcactga
     1561 atactgttac ggatcgcttc tccctggtag agaaaatccg caaacacaca ccgcagaaca
     1621 accgaaatta cgtcattcag tcggtacgag acacattcaa ctcgccagaa acaaaagagc
     1681 gtatcgcgct gggtgagatg tatggttatt acggtcatgg tcgccgggcg atgcactata
     1741 acaaaacgaa aagcctgaac ttgccggaag tctctgttgt gatggtagat gggaagcctg
     1801 ttgtattgga gaacgtgcca tccaacagga ctatcgacat ttcgattgac gacaacggca
     1861 ttgttaccca tacgcaggaa atactggata cagacaccgg gcgtattgtt caggggatga
     1921 taaattctgg cgccggtggt tggtcatggg ctacatctgg tccagactca tcggtatccc
     1981 tggtaaagtc tttccacggc tttgattacg taactgtgcc taactatatc agcctcgaca
     2041 agaaaagcct gatgcttgaa tcagcagaag agcgcgatgc ggctattcat gcagcactta
     2101 tcgaacaagg ttttaccgat aacgctgcca cggatctggt tcaccacttt tcagcgatga
     2161 aaaatcagca agccatgctc gagtctgcac atcgtgaagc cctggaatca gaattaatgc
     2221 tccttgaagc ggaaaatatg caacttcgcg acaaattacg aaaccaggct gcaatgatgg
     2281 aaagccaggg cgaagatgcg aagaaatgcc gccgtatttt gcgtgatgct atccaggaaa
     2341 tgcctgtttt tatcagtgca gaacaacgcc gggcgctctg ccgtatgcag tctgaagatg
     2401 atgctcgcat agtggctgcg atgcttgaat ctctgggggc aaatgcgact tatggcttac
     2461 cgatatcagg caagaaagag gcggagttat caccgaagga gaacaaaaat accacaccgt
     2521 tactcttcgt ttcgcgacgg gcgtaaccgt tacataaagt taaatttcta acatagtgta
     2581 tttatataca ctgttgatcc tgttcgatgt gatcgagtag gatcgcggtc actgctccgg
     2641 tagcgactgc caaaaaagca aaagccgctg agaagcggct tttgggtgta taaatcctcg
     2701 gacatagatt tatacggtag atagattttt ttcacggaac tatctggtga gtattttaaa
     2761 ttcagtttca gtaactttca acacttctga gtataaaaac aacactgaat accacaaaaa
     2821 aatcaaaggt catcagttga gtttttttgc tcgccactgt ggttttcttt ccccttcaca
     2881 tagcgccatt attgccgagt tcgccaacct ggcaggggct accgatgaat acatgatccg
     2941 tcgttcatat gctgatatgt ccgagattac aggacgcagt atttctactg tgcgccgggc
     3001 atttgctgaa gcgacgaagt gcggaatgct tgttaagcaa catcaggtag cgaataacaa
     3061 tgctcaggtg tgtaacgtat accggtttac aacacaattt ctccacttca ttcatgtagc
     3121 aatggagata gggggtaagc agggcatcaa atttgcgaac gccacaaaac tggttaagca
     3181 gttgatttca aatgttcggt atttttttga aactgggaac cccctgttca aattgaacaa
     3241 gtccccccat gttcaaaatg aacagccaat agaaaataag agtcactcta tagccaaaag
     3301 aagagaaaga tcatgcgcgg ttcagccaaa ggcttcacca gcagatagtt cccaagcgga
     3361 taatggatta ctggataaaa aaccagtaat cagggaggaa cataccaacc actgcctggc
     3421 ggcggcaaaa gcgagagctg caaagcgtcg ttcagacgag gggcacgcaa aacggcaggc
     3481 tctgtatcgt accgctgaaa agctggcaaa aaaatttgca tggatacgta gtgccgctac
     3541 cgcagtgaat aagcctaaac aatccagtgc cctggatttc tcaatggatt attctggctc
     3601 ccaggggtgt gtaaccattg gtgaagcctt tgacctgatg aaacagcgtg gctatcggtc
     3661 tgaatttgac cgggaggatt ggtcaatccc tgcgggattt aggggatgac ctgtgtgtct
     3721 acaaagcatc ccaatgatcg gaaaatgctc gctcgatgat ttgtgttaga aaattaattt
     3781 gctgtttcta aatcggaatg tatgatctgc atcatcgact gaagtcatcc attgctgaaa
     3841 tttttagttg atcttccatg ttttcaggct tatattcatg ccgtcgtagc aaattctgcg
     3901 accaggtttg acagcctgaa tgttagtgcg gacaaccgca gatttccgat attgcggtat
     3961 ttttgtgtcc gtaaaccgcg ttacgcccga attatggtgg ggcgtgatgg ggaggcttcg
     4021 gcctgctggt ttcactaacg ccagtctgtc aaccctgtca cgtcctgcca cctgtttgac
     4081 agcgggtagc aggttgttaa acctgttagt gaggccgtaa ctatggttaa tgccaatcct
     4141 tgctcacgtc cagaattcat ctggcgtttc cactccctgc aaaaacactg cgatcacttc
     4201 gcaattgccg caaccgaaaa agaagcccgt tctctgatcc ctgatcagca tggtgttctt
     4261 actggtcgtt tctctacttc cccacaacca aacactaatc cctggtatcg caatattacc
     4321 aatcccggca ttgcggaggc acactgatgg atatctgcat tcttgaaaag ccatcaatga
     4381 ccagcattga gatcgcggag ctggtgggaa aacgtcatga caatgtgaag cgtaccattg
     4441 aaacattagc cgaatccggc gttattgttc gtcctcaaat tgaggatgaa ccaaaaatag
     4501 atgtaatggg gcgttctcga ttaattcagg tgttccgatt tagcgacgag caaggcaaac
     4561 gagacagtat cattgtagtc gcacagcttt gtcctgagtt tactgcccgg ctggtggacc
     4621 gctggcgcga actggaagaa caagtacgcc agccgttgac cgaaatagag atgatcgccg
     4681 caatggctgc aaatgcagtt cagcagcaaa agcggcttca tgttgttgag tcaaaggtta
     4741 gccaggtggt agaaaccgtc gagcaaatca agaaaggcaa tattccggaa ggctacattg
     4801 gctaccgcca gctggcggcg aaatgcggcc tgactgaagc caaatgtcgc aaccttgtca
     4861 acgcttaccg gattcccacc gatacacatg agtttttaac tccagagggg gtattgtcgc
     4921 gtcgttccat cgtggcctta tctcccttca tgaatgcttt taaccggatg atgtcggagg
     4981 ctgaacaccg aggaaaacgc tggtatcacc ccaaaatggg gcaattccag gtgatcggtt
     5041 ggggaggtga gtgatggaca tcatgcaggc ggtaaaaaaa atcagggaag gacaggctga
     5101 agtgtgccgc cgtaaagcat ggagtgttgg tgtatgggcc tggcctgata attactctac
     5161 taccggatgg ttttgtatta atggactcca gatccgggaa gttgatttat cgacggctga
     5221 tatagtcggt aatgattgga tcgtgacttg aagcgtgatg ccccggcctg gccggggctt
     5281 ttttcatgcc tcattagttg aggttgctgc gtctccgact ggtaacagag cggtattgcg
     5341 tggttcgcca gcaggccagc gatagccaga cactcttgat gttgggaatg cgcgaatatt
     5401 aaccgcatcc ccctggttcc cacccaggac aagcagatcg ccattggcgc ggcgtccgac
     5461 aacgaagcct acgtgcccac cgcctacgcg ggtaaataca acgatacatc cgtatgccgg
     5521 tgtatctaat ttctcgcccc aatccagata ggattttgcc gactcaaatc ttgttgagcg
     5581 gatgcctaca cgttccagca ttgcaccgac aaatgctgcg caccacggag tttcatcgtc
     5641 tttaattccg ccgcgcttga tgtcgcgcca gaactgaacg atttcagggt tgtgtttagc
     5701 accttttatt tcagtcaggc caatgtgctt acgggcttca attagccagc gaggttcaat
     5761 gtttgccata tttttctccc ggtcatgggt taataagtag gcaaactatg gggagtatgt
     5821 gatttagacg gtagtatcgt ccccgtgaaa ataacattct ccataccctc tcaaccgtac
     5881 cgcatagcgg attttttacg gtttagaggg ggtaatcctt tggataaact acttcattca
     5941 atcagtgaag cctccagctt aggcgaactg attgaaattg ttagtcggtt gcataaatta
     6001 cgtgctgttg cgacctttgg cgttaagaaa gtcgatggtg tcagcattca aaaaacacgg
     6061 cgggcagcaa acaacgccgc agtagagctt cttaattccc ttccgcccgg cttcgatggg
     6121 gcgaaattaa ctgacgaaca acgtcagatt cttgccgggt ataccggtga aggcggcctg
     6181 acagatggtg agggcagcca atacgaatac tacacaccac agtttatggc tgaaggtata
     6241 tgggatctgt ttgctgacta cggtattgat ggcgggcacg tactggaacc atcagcgggc
     6301 acaggtattt tccaggagac aaaacggcag ggcatgataa tgaccagcgc cgaactatct
     6361 ccgatttccg gtcgaataaa ccagcttttg cacccggaag acgatgtgaa tatcggggcg
     6421 tttgaggcgc tggcggcgaa agacgcaatg tatgaccacg ctgttggtaa cgtaccgttt
     6481 ggtgaagggc gttccggcgt tgccgggctt gatccggcat atgcaaacga gaagaacgtt
     6541 ggcaattact ttgtgctgcg cacgatcgac aaggttaagc ctggtggact aattgtattg
     6601 gtggtgccga atggcatgac cgatggtacg aaatacaaaa aattgcgcga taaagtcagc
     6661 cgtaaagcgg aatttttggg cgcacatcgt atgccttctg gtacgttcag cgaatccggt
     6721 acagatacag tggtggacgt atgggtattg cgtaaacacc cggaaacctt cctggagatg
     6781 atccccgaca cggatgatgc aacgctcaag tcggcaaacg tcctttggga taccttcctc
     6841 aaagggaaat ggtttacaac cgaagggaag cgatttgttt acggcgatat ggagcgtacc
     6901 agcttccgca ataccctggt ggtgaaaaag gacggtcgtg tatccaatga gtcgatgaaa
     6961 aacgctctat cgcgccgctt tgaaagccgc attaattggg atttgcttgg cgtaactact
     7021 caggcatggc agggcgcgaa agtgggcgat aaacgccttg taggcggcat ctggcatgag
     7081 tttgatggcc tgaaatgggt caaggacgcg acaaccaaat cctccgcgct tgatgtaaat
     7141 cgatatggtg ttacgacgtt tggtgatctg cagatcgcgt ttcagtcaac aaatggcatt
     7201 ctggcgttgt catgggacca gatatcggcg atcgcgtccg actatccgtc ggttatcagt
     7261 gatgaagtag cggcaatgat tcgctttgct ggcaagcaga gagaaaaaga cagagaacgc
     7321 gttatgcgcg gcgcactgat agggcagttg atcaacaagg cgcttgataa gcgtaatctg
     7381 ggggaaaatg ttgatgatga acttgctgat gccgcgcgcc tggcggaagc cgaaattgcg
     7441 aagtatggtc cgccgcacgc gattaagctg aatgggattg ctgaagctgg cgctaaaaac
     7501 tggatgactt tcactggcaa cgtgaaacag gacggctctg cttctgacct ccttgctgga
     7561 cgacttgatg taacggatgg tgctgccggt attgacttta cacgcccgga acaagttgta
     7621 acccaccttt tcagtgacgt ggcacttaat ccaatcacac tggacgattt tcgtgaggcg
     7681 tttgctggcg aactgccaga agacgaagat gctgctctgg aatacctggc gaaatttgat
     7741 gatatcgcta ttgatgggta tggctgcctg ctgccgatgg accgcgccac cagcggcgat
     7801 attgcaacca aaacagcatt gctggtgggc tggcgtgatg cgtcaacagg tgagcagaaa
     7861 gcgaactttg agcgtcagtt ggcgaaaatc gaagagaaac gcattttcac gccactaaat
     7921 aaagtcaccg ttaatctgaa cgcccgctgg ctggatcgcc gcttgataaa agagttcctg
     7981 gcagagcagg gctatgacga gtttaagtat acagaacccg accttaaggt tgaggacggc
     8041 attctggtat ctccggacga ttacgaaggt aaggacgggg tatttactgg ctatcaacta
     8101 cgaaccgtaa acggcaaaaa cgggaatgag ttcaagaagg ccaacaataa agacggcttc
     8161 ctgaaccagt tagaaaacta cctgaacggc attaaaccgc gtgggcagaa cgctaacgag
     8221 tacctggata agattgctca actggaaatt agcttcaacg actggttacg cactcacccg
     8281 caggctgatc agatagcccg cgactataac gacgcattta atggcttcat cccgtttacg
     8341 cattctgacg caccgcttgg cctggagggg attagtggga agcgcattcc tctgacttat
     8401 cagaatgaag aagttcggcg cttgtcagaa gatgggcgcg gcatcatggg atttggtacc
     8461 gggctgggta aaaccacgac cgcgcttgcg cttgaggcgt ataacttcga aacaggtcgc
     8521 actaaacgca cgtgtattgt tgttccgaag gcggtttatc agaactggta tcacgaggca
     8581 cagagcttct acagtgctga tgccttcgcc aatatgatgt ttatcggtct ggatgaagtt
     8641 cgtgatgaca gcggaaatat tctgaccgcg ccggtgcttg atgaaaatgg cgaaccacgc
     8701 ctcgacagta acgggcagcc tcttacccga aatgttgtga aagagtccgc cagcgccgtt
     8761 atcgttcagc gcatgaacat gatcccgtcg tcaaattggc gaacggtcat catgacaaaa
     8821 gagcaattcg cgtctatccc gctacgagaa gaaaccattg aggaaaactc acagcaagcg
     8881 gtctttaatg ctgtggaaat ggggcgtctt gacctggcat ctggcaaaca ccgtgacgcg
     8941 cagaagaaga acaagattaa ggaccaggcc gcaaataccg gtacaacgaa gaagcagaat
     9001 atcccgtact ttgaggatat gaacttcgat agcgtaattg ccgacgaagg gcataactac
     9061 cgcaactcct ttagcgccgg gcgcgaagcg gggcaactgg catatttgcc taacccggca
     9121 gtatccaaaa tggcccgcga catggctgta aaagctgcgt acatgatgaa acgcaacaac
     9181 gggcgtggtg tggttatgct gacggcaaca ccgctggtga acagcccgat tgatgctttc
     9241 aacatgctgt ctactgtcat tccgcaggaa gagtggatgc gcatgggtat tgttacgccg
     9301 gatgatttcg ttcgcgtgtt cggtaaaaca gccacagttc aggttcagaa aatttccggt
     9361 gaggtggaag agaagcaggg gctggtgggc ttccagaacc tggatggtct gcgtggtatt
     9421 ttccatcgct ggactactct gaagactgct gcggacgtcg gggcgacggt aaaaatccct
     9481 gatattgtcg agaatacctt gcagatcccg atgacaggag agcaagaggc catttacgaa
     9541 gaacttcgta agcgtgcaca ggagttaagt agtaaagatg cgctgacggt cgatgaaaac
     9601 gggcatatca caaacgaaaa accggatgat ttcatcttct ccatcatccg cgacatggat
     9661 aaggttgcta tagatcctga tctgtatgcc agtgcgatta cattccagtt ccccgttgag
     9721 cttgcagaaa aggtaaaagc tattgctgat gccctgccaa aagtagctgg cggtaaagtg
     9781 gcagatgatg cagacgaggc cgaagaagat ggcgctggtg ggctggtcag tacgcgtacc
     9841 agcaaggtag tgaagaccac ctttagcgaa catccgtcgc atgttgaaat acgtgctggc
     9901 attgaactgg aagctgaaat actgaaggcc attgcggctg ccggtattga tatgcagcag
     9961 gtatcacacc ccatcccgcc gaagtatgcc gcgctgatcg aaaaccttcg tgaaggtctg
    10021 aagaacggta aacaaatcgt cttcattgat gaaaaagccc agcaccagaa gctgcgccgt
    10081 attattgcca gtgcactgca aatgccggag caggagatag gcattatcaa tgcgacgaca
    10141 gttagccagg ctggcggcgt caagctgaag aaggtgaata agccgactga acctacgcca
    10201 aataaaaacg gtgaatacaa agaaggtgca tgggaaacct actacagcaa acttgcccaa
    10261 tatgaggatt atctctcagc taaaaatgat gctggccttg agggcatgga aggtattgcc
    10321 gccgactata acgaaggtcg cacgcgcatc ataatctgta acaagaaggc tgaagtaggt
    10381 attaacttgc acatcgggac aaccgatatc caccatctga cactaccctg gactcctgcc
    10441 agtatcgacc aacgtaatgg gcgcggcgct cgggtaggct caccacaaga aaaggtcaat
    10501 gttcactact attgtggcaa aggcacattt gatgacttcc gtctggatac gctgaaacgt
    10561 aagaaggact ggatcaatat ggtgatgacc tccgacatgt cagagatcgc caatggtgat
    10621 gcggatgacg cagatgaacg cgccattatg ctggcggcaa atccggaaga acgccgtgca
    10681 atcatgagtc gtcaggcgca agagcgtgaa gaacgtctga aactgaaagc cctacgcgaa
    10741 gccaataatg ctctcgataa ctacctgaaa gcggcgaatg ctgccgggaa agatatcggc
    10801 atgatggaga cagaactgaa aagcgcgatg gagcaggtag agcattacca gcgcaatctt
    10861 gacgacctga ttaaagcggg gacgaacagg aacgggcaga aatatgttct ggaacagttg
    10921 cgagactaca gaaaacgtgt gcgggagcta cgctttgcga tcaccagagc taaagacgct
    10981 ggcaccgtta tgaaacgttc tcgtggtgat ctggaacgtg cgattaaagc cggggttctg
    11041 gagcttgatg ccgatgtatt gcagaaccca caggagtatg tacgcactga gaaaaatatc
    11101 ttgttgcaca agggcagcca ttatcgcgcg atcatcgatg agaatcacaa tacgacagca
    11161 attgttcgtg ttaccgccat tgatgcggaa aggggcgggt gtacgtgccg catatcctgg
    11221 ctggatgtgg ctagtcgtta ctctcgttct ccggggacgg cattattttt gccctttaac
    11281 ctgataattg aatcggtaac ctttgaagag ggagttgcag aatcgcgtga aacagctgca
    11341 cgtggtgttc aatgccacca gctatcaaca acacttacgc gcgatcagtt ctatgatgct
    11401 attcgcaatg gggtaatgaa tgtcactgcc gggaagcctg gtagagtatg gggcggtggt
    11461 tcagctatta aatactgggc ttatcgcaca gatgatggtc agatcgcgct gtcatcgacc
    11521 tatgatggcg tgataggcca aaaaatcggg cgagcatcgg atatagcacc tgagcgatgg
    11581 atatatcctg atggcaacga cgaggcgctg aagcgtgaag tggcaaaaat acaagccagt
    11641 gataacccgc tgaatgtcgt ggacggcgag ggattcttcc aggcattgta tggatatgat
    11701 tacatccagg gaatgcaggc atggggggca caggccacga tggatgatgt ttcccaggag
    11761 tttgaaaact ggatggcagg gcgtggtttg tccggtcgct cacttaataa agttacttct
    11821 gaagatattc ttagcctgtt ccgtaaaccg tccggagaag ggctggaatc tttctggaag
    11881 accgtggtgg cataccgttt attccagggc aggatgaaag cctttagcaa cactgatgac
    11941 attgaacgga tgtttaatca ggtgaaggcc gcgaaaatta cggcgaaaat tgagaaaaca
    12001 cgagctgcgc tggtggcctg gcgcgatgca ttgtttgagc aatataaatc actcagcgat
    12061 gccgatggct ggtcggcatt ggagcagctg gctaataatg gtgtcgatgg agcgaaatta
    12121 gccattgttc aggccgataa tcctctggat tcgccagcag cgcagtggat taaggtgggg
    12181 gctgtcatca atgcatttga tgaaaaacac attagtaagg acgattttgc tgatgctgtt
    12241 tccgttaacc gcctggtcga aatggctgtc cgccgtgctc gtgctttgaa agacggggac
    12301 tatgtgaatg agt
//
LOCUS       DBBGLQ010000125        11856 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.125, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000125 DBBGLQ010000000
VERSION     DBBGLQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.125"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            57..1664
                     /locus_tag="ACIYSB_004518"
     CDS             57..1664
                     /locus_tag="ACIYSB_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhiJ/YhiL family protein"
                     /protein_id="HGC5431425.1"
                     /translation="MKAIDNQIRNISSSHQDKHSDKVNSHQHHGKVDKTHRAKIVEFD
                     KLDNDSQIDNDFGLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDIIYKVDGLSIIARNF
                     FTLLSGNFKPDWRWDVSKETFTKEKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYKHKNNKVLYDLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCNYDSERQVLTSHHGI
                     IRDEEKKCYCLGALQVPFNQMKNPTDPEELANIFNKKHKEHMDDPLFHLIRSNTLKPT
                     PVYANYFDNTTDFRPARERIFICGETLKGEDPSKYIRQKYGHHGPGVDHNQQFDNGIM
                     GLNSLKEARDKNNKIIYSSGLSCFQPH"
     gene            1926..3548
                     /locus_tag="ACIYSB_004519"
     CDS             1926..3548
                     /locus_tag="ACIYSB_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGC5431426.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     NNNYQFIVTINSKNYGCSLDNTDVNWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETMIKPQFADRIYFSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLNTNVNTIISILNRRDIILESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTGYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3738..3851
                     /gene="yhiY"
                     /locus_tag="ACIYSB_004520"
     CDS             3738..3851
                     /gene="yhiY"
                     /locus_tag="ACIYSB_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGC5431427.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGHICRFLLIPDGRIR"
     gene            3914..4981
                     /gene="yhiI"
                     /locus_tag="ACIYSB_004521"
     CDS             3914..4981
                     /gene="yhiI"
                     /locus_tag="ACIYSB_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HGC5431428.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4978..7713
                     /gene="rbbA"
                     /locus_tag="ACIYSB_004522"
     CDS             4978..7713
                     /gene="rbbA"
                     /locus_tag="ACIYSB_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGC5431429.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLVSGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7713..8837
                     /gene="yhhJ"
                     /locus_tag="ACIYSB_004523"
     CDS             7713..8837
                     /gene="yhhJ"
                     /locus_tag="ACIYSB_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5431430.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(9002..10138)
                     /gene="yhhI"
                     /locus_tag="ACIYSB_004524"
     CDS             complement(9002..10138)
                     /gene="yhhI"
                     /locus_tag="ACIYSB_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGC5431431.1"
                     /translation="MELKKLMEHISIIPDYRQTWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGTQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            complement(10279..10629)
                     /locus_tag="ACIYSB_004525"
                     /pseudo
     CDS             complement(10279..10629)
                     /locus_tag="ACIYSB_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
     gene            complement(10734..11117)
                     /gene="yhhH"
                     /locus_tag="ACIYSB_004526"
     CDS             complement(10734..11117)
                     /gene="yhhH"
                     /locus_tag="ACIYSB_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HGC5431432.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            complement(11089..>11856)
                     /locus_tag="ACIYSB_004527"
     CDS             complement(11089..>11856)
                     /locus_tag="ACIYSB_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HGC5431433.1"
                     /translation="LISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGAC
                     TNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTA
                     PNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIK
                     SFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
BASE COUNT         3138 a         2681 c         2746 g         3291 t
ORIGIN      
        1 tcagacaagt atcatttatt ttgttacaaa caattcaaaa atatcacagg ttatttatga
       61 aagcaattga caatcagatt agaaacatct cttcaagcca tcaagataag cattcagata
      121 aagttaacag tcatcaacac catggcaagg ttgataaaac acatcgtgca aagatagttg
      181 aatttgataa actggataat gattcgcaaa tagataacga ttttggcctg catatcatct
      241 atttcctgca acatggtcac tggaaggtaa atgatcgtag tcaccagatg gagaaagtat
      301 ggttttataa tagtgaacct tctatagata ttcaagaata taatagattc gccgacaata
      361 ctactgatac gtttatcttt acaattatac cagacaataa ccatgtgtta aaattatcgt
      421 ctcccattac cgttactgtt gagtgtaagg gcggttatta ttttattaat tcctctggag
      481 ataaatcaga tataatttat aaagttgatg ggctttctat catagcgaga aatttcttta
      541 ctcttcttag tggtaatttc aaacctgact ggcgttggga tgtctctaaa gagacgttta
      601 ctaaggaaaa gtttgatagc tatgttaaat ctgtgttttc aaaaatagac ttctataaac
      661 agtgtggtgt gattaaccca caaaatgcca acacggctta ttttggcgat acagatggaa
      721 gagtaggcgc tgtactttat gcattgctcg tttcagggca tataggcatc agggaaaagg
      781 gttggagttt attatgtgaa ttattaaaac acgaagagat ggcttcatct gcatacaaac
      841 ataaaaataa taaagtgctc tacgatttat taaatacgag agacatgatc ttgaatgagt
      901 tgcatcaaca tgttttttta aaggacgatg cgataacccc ttgtatcttc cttggggatc
      961 atacaggcga tagatttagt actatttttg gtgataaata tatccttact ttgctaaatt
     1021 ctatgcgaaa tatggagggt aataaagata gccgtattaa taaaaatgtc gtcgttctgg
     1081 ctggaaatca tgaaatcaat tttaatggta actatactgc acgactagca aatcataaac
     1141 tctctgccgg tgatacgtat aacttaatta aaacgctcga tgtttgtaat tatgactcag
     1201 aaaggcaagt tttaaccagt catcacggaa taataagaga tgaagaaaaa aaatgttatt
     1261 gtctcggtgc tttgcaggtt ccatttaatc agatgaaaaa tcctactgat cctgaggaat
     1321 tagccaatat ttttaataaa aaacataaag aacacatgga tgatcctcta ttccatctaa
     1381 tccgaagcaa tactttgaaa cccacacctg tatatgctaa ttattttgat aacactactg
     1441 atttcagacc agcacgtgaa cgtatcttca tatgtgggga gacactcaaa ggtgaagacc
     1501 ccagcaaata cattagacaa aaatatgggc atcatgggcc cggggtagat cataatcaac
     1561 aatttgataa tggtattatg gggctaaatt ccctgaagga agcacgtgat aaaaataata
     1621 aaataattta ttcctcaggg cttagttgtt ttcaaccaca ttaaaactcc atgaatacgg
     1681 tggtatgtct ttttaatcct gccagtattt cataggtttt cttaatcgat tgtagaggca
     1741 tgttctgatg acgcctctac aatctttagg gactttctct gctatgagat aagaaatttc
     1801 atattatgct ttgaggctga atgatttgtg caaaatataa ttattttccc atcgaacatg
     1861 aggtgtatgt cataagataa aatgttaact atgtattcct cgcggtaaat aatatgaggt
     1921 aaaatatgaa aattggaact gtggcaggca ctaacgacag tacaacgaca atcgcaacaa
     1981 atgatatggt gcaagagcat gttaccaact tcactaaaga actgtttggg tatattgcaa
     2041 atggtatagg tgatgatata tcaagtattg ccaggacaat gctcggtgag gttgtggaaa
     2101 aaatagatga ttggcaaata gaacggttcc aacaatctat tcaagatgac aaaatttcat
     2161 ttaccataca aacggatcat tcagaaaaat atagtatgct atcgggcatg cgtgctcata
     2221 ttctacgtag gaataataat tatcagttta ttgtgacaat taatagcaaa aattacggtt
     2281 gctctcttga taataccgat gttaattggt gcagtattgt ttatttactt aataatatga
     2341 cagtaaatga taatgctaat gatgttgcag ttactgagtc gtacaaaccc atctggaact
     2401 ggaaaatatc gcaatataat gtatctgata ttaaattcga aacaatgata aaaccccagt
     2461 tcgctgacag aatctatttc tccaattgtt tacccgttga tcccacaagt accagaccaa
     2521 cctattttgg tgataccgat ggctcagtcg gtgcagtttt gttcgcgctt ttcgcaacgg
     2581 gacatttggg aatcatggct gaaggagaaa atttccttag ccagttgctt aatatagaag
     2641 atgaagttct caacgtattg ctaagggaga actttaatga gcaacttaac actaatgtta
     2701 atacaataat cagtatcctg aacagaagag acattatttt agaaagtctt cagccttatt
     2761 tagttattaa taaagacgct gtgacgccat gtacgtttct gggcgaccaa accggcgatc
     2821 gatttagcaa tatctgtggt gatcaattta tcatcgattt gttaaaacgc ataatgagta
     2881 tcaatgaaaa tgttcatgta ttagctggaa atcatgaaac gaattgcaat ggtaactata
     2941 tgcagaactt cacgcgtatg aaaccactcg atgaggatac ctactctggg ataaaagatt
     3001 atcctgtatg tttctatgac cccaaataca aaataatggc aaatcatcat gggataactt
     3061 ttgatgatca gcgaaaacgt tacattatag gaccgataac agtctctatt gatgaaatga
     3121 ccaatgctct cgatccggtc gaattagcgg caattatcaa taaaaagcat catgctataa
     3181 ttaatggcaa aaagtttaaa actagccggg ctatctcttg tcgatccttt aatcgttatt
     3241 tttctgtttc gacaggttac agacctaaac ttgaagcact attagcatgt tcacaaatgt
     3301 taggtataaa tcaagttgtt gcacataacg gaaatggtgg aagagaacgc ataggcgaaa
     3361 cggggacagt tttaggtctt aacgcccgtg acagtaaaca tgctggaaga atgttcagca
     3421 tgcataattg ccaaattaac cctggtgcag ggcctgagat aaccactccc tggaagtctt
     3481 accagcatga aaaaaacaga aatggactta tgcctttgat tagaaggcgc acaatgttac
     3541 agctctgaac catagacaac atgaatatta atgttactat ctggttgaga ctgttgataa
     3601 aacgtaaaaa ggaatgctct gttcctgcaa ctgaacaggc gtgaaacgct atccaacagg
     3661 atggataccg ttttgtcagc cagttttata gatcacactt atgagtatgc gtgtttcatt
     3721 tacggccagg aatgctgatg atgacaacat tactacccgt tttcactaag ccttccccgt
     3781 tggcgctcaa tgctctgcgc gctggtcata tttgccgttt ccttcttatc ccggatggaa
     3841 gaatccgctg aacgtcgttc ggcggcagtt gtaacacgcg tcgtttgctt acagggatta
     3901 tggcgggtcg attatggata agagtaagcg ccatctggcg tggtgggttg tcgggttact
     3961 ggcggtggcg gctatcgtgg cgtggtggct gttgcgcccg gcaggtgtgc cggaaggctt
     4021 tgctgtcagt aatgggcgca ttgaagcgac ggaagtggat attgccagca aaattgccgg
     4081 gcgtatcgac accattctgg tgaaagaagg ccagtttgtt cgcgaaggtg aagtgctggc
     4141 gaagatggat actcgcgtgt tgcaggaaca gcgactggaa gccatcgcgc aaatcaaaga
     4201 ggcacaaagc gccgttgctg ccgcgcaggc tttgctggag caacgacaaa gcgaaactcg
     4261 tgccgcacag tcgctggtta atcaacgcca ggcagaactg gactccgtag caaaacgtca
     4321 tacgcgttcc cgttcactgg cccaacgagg ggctatttct gcgcaacagc tggatgacga
     4381 tcgcgccgcc gctgagagcg cccgagctgc gctggaatcg gcgaaagctc aggtatcggc
     4441 ttctaaagcg gctatagaag cggcacgcac caatatcatt caggcgcaaa cccgcgtcga
     4501 agcggcacaa gccactgaac ggcgcattgc cgcagatatc gatgacagcg aactgaaagc
     4561 cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca ggcgaagtgc tggcggcagg
     4621 cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat atgactttct tcctgccaac
     4681 cgaacaggcg ggcacgctga aactgggcgg tgaagcccgg ctgatcctcg atgccgcgcc
     4741 agatctgcgt attcctgcaa ccatcagttt tgtcgccagt gtcgcccagt tcacgccaaa
     4801 aaccgtcgaa accagcgatg aacggctgaa actgatgttc cgcgtcaaag cgcgtatccc
     4861 accggaatta ctccagcagc atctggaata tgtcaaaacc ggtttgccgg gcgtagcgtg
     4921 ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctc gtggtgaggt tgccgcaatg
     4981 acgcatctgg aactggttcc cgtcccgcct gtcgcgcaac tggcgggcgt gagccagcat
     5041 tatggaaaaa ccgttgcgct gaacaatatc actctcgata ttccggcccg ctgtatggtc
     5101 gggctgattg gcccggacgg cgtcgggaag tcgagcttgt tgtcgttgat ttccggtgcc
     5161 cgcgtcattg aacagggcaa tgtgatggtg ctgggcggcg atatgcgcga cccgaagcat
     5221 cgccgcgacg tctgcccgcg catcgcctgg atgccgcagg ggctgggcaa aaacctctac
     5281 cacaccttgt cggtgtatga aaacgtcgat tttttcgctc gcctgttcgg tcacgacaaa
     5341 gcggagcggg aagtgcgaat caatgagctg ctgaccagca ccgggttagc accgtttcgc
     5401 gatcgtccgg cagggaaact ctccggcggg atgaagcaaa aacttgggct gtgctgcgcg
     5461 ttaatccacg acccggaact gttgatcctt gatgagccaa caacgggggt tgacccgctc
     5521 tcccgctccc aattctggga tctgatcgac agtattcgcc agcggcagag caatatgagc
     5581 gtgctggtcg ccaccgccta tatggaagag gccgaacgct tcgactggct ggtagcgatg
     5641 aatgccggag aagtgctggc aactggcagc gccgaagagc tacggcagca aacgcaaagc
     5701 gctacgctgg aagaagcatt tataaatctg ttaccgcaag cgcaacgcca ggcgcatcag
     5761 gcggtagtga tcccaccgta tcaacctgaa aacgcagaga ttgccatcga agcgcgcgat
     5821 ctgaccatgc gttttggttc cttcgttgcc gttgatcacg ttaatttccg cattccacgc
     5881 ggggagattt ttggttttct tggttcgaac ggctgcggta aatccaccac catgaaaatg
     5941 ctcaccggac tgctgcccgc cagcgaaggt gaggcgtggc tgttcgggca accggttgat
     6001 ccaaaagata tcgatacccg ccgtcgggtg ggctatatgt cgcaggcgtt ttcgctctat
     6061 aacgaactca ccgtgcggca aaaccttgag ttacatgccc gtttgtttca catcccggaa
     6121 gcggaaattc ccgcaagagt ggctgaaatg agcgagcgtt ttaagctcaa cgacgttgaa
     6181 gatattctgc cggagtcatt gccgctcggc attcgccagc ggctttcgct ggcggtggcg
     6241 gtgattcatc gcccggagat gttaatcctc gatgagccta cttctggtgt cgatccggtg
     6301 gcgagggata tgttctggca gttgatggtc gatctctcgc gccaggacaa agtgactatc
     6361 ttcatctcca cccactttat gaacgaagcg gaacgttgcg accgcatctc actgatgcac
     6421 gccggaaaag tgcttgtcag cggtacaccg caggaactgg ttgagaaacg cggagccgcc
     6481 agtctggaag aggcatttat cgcctatttg caggaagcgg cagggcagag caacgaagcc
     6541 gaagcgccgc ccgtggtaca cgacaccacc cacgcgccgc gtcagggatt tagcctgcgc
     6601 cgtctgttta gctacagccg ccgcgaagcg ctggaactgc gacgcgatcc agtacgttcg
     6661 acgctggcgc tgatgggaac ggtgatcctg atgctgataa tgggttacgg catcagtatg
     6721 gatgtggaaa acctgcgctt tgcggtgctc gaccgcgacc agaccgtcag tagccaggcg
     6781 tggacactca acctctccgg ttcccgttac tttatcgaac agccgccgct caccagttat
     6841 gacgagcttg atcgtcggat gcgtgcgggc gatatcacgg tggcgattga gatcccgccc
     6901 aatttcgggc gcgatatcgc gcgtggtacg cctgtggaac tcggcgtctg gatcgacgga
     6961 gcgatgccga gccgtgctga aacggtaaaa ggttacgtgc aggccatgca ccagagctgg
     7021 ttacaggatg tggcgagccg acaatcgaca cccgccagcc aaagcgggct gatgaatatt
     7081 gagacgcgct atcgctataa cccggacgta aaaagcctgc cagcgattgt tccggcggtg
     7141 atcccgcttc tgctgatgat gatcccgtca atgctaagcg cccttagcgt ggtgcgggaa
     7201 aaagagcttg ggtcgattat caacctttac gtgaccccca ccacgcgtag tgaatttttg
     7261 cttggtaaac agttgccata catcgcgctg gggatgctga actttttcct gctctgcggc
     7321 ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt tcctgacgct caccctggcg
     7381 gcgctgctgt atatcatcat tgccaccgga atggggctgc tgatctccac ctttatgaaa
     7441 agccagattg ccgccatttt cggaacggcg attatcacgt tgatcccggc gacacagttt
     7501 tccgggatga tcgatccggt agcttcgctg gaagggcctg gacgttggat cggcgaggtt
     7561 tacccgacca gtcattttct gactatcgcc cgcgggacgt tctcgaaagc gctggatctg
     7621 actgatttgt ggcaactttt tatcccgttg ctgatagcca tcccgctggt gatgggctta
     7681 agtatcctgc tgctgaaaaa acaggaggga tgatgcgcca tttacgcaat atttttaatc
     7741 tgggtatcaa agagttgcgc agtctgctcg gtgataaagc gatgctgacg ctgattgtct
     7801 tctcgtttac ggtgtcggtg tattcgtcag cgaccgttac gccaggatcg ttgaacctcg
     7861 cgccgatcgc cattgccgat atggatcaat cgcagttatc gaaccggatc gttaacagct
     7921 tctatcgtcc gtggtttttg ccaccggaga tgatcaccgc cgatgagatg gatgccggac
     7981 tggacgccgg acgctatacc ttcgcgataa atattccgcc taattttcag cgtgatgtcc
     8041 tcgccggacg ccagccggat attcaggtga acgtcgatgc cacgcgcatg agccaggcat
     8101 ttaccggcaa tgggtatatc cagaatatta tcaacggtga agtgaacagc tttgtcgcgc
     8161 gctaccgtga taacagcgaa ccgttggtat cgctggaaac ccggatgcgc tttaacccga
     8221 acctcgatcc cgcgtggttt ggcggggtga tggcgatcat caacaacatt accatgctgg
     8281 cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga acacggcacg gtggaacact
     8341 tactggtgat gccgataacg ccgtttgaga tcatgatggc gaagatctgg tcgatggggc
     8401 tggtggtgct ggtggtatcg ggattatcgc tggtgctgat ggtgaaaggt gtactgggcg
     8461 taccgattga aggctcgatc ccgctgttta tgctgggcgt ggcgctcagt ctgtttgcca
     8521 ccacgtcaat cggcattttt atggggacga tagcgcgttc aatgccgcaa ctggggctgc
     8581 tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg tggttccacg ccgcgcgaaa
     8641 gtatgccgca gatggtgcag gacattatgc tgaccatgcc gacgacacac tttgttagcc
     8701 tcgcgcaggc catcctctac cggggtgccg gattcgaaat cgtctggccg cagtttctga
     8761 cgctgatggc aattggcggc gcatttttca ccattgcgct gctgcgattc aggaagacga
     8821 ttgggacaat ggcgtaaata caattgcccg atgcgtaagc ttatagggac tacgcatcgg
     8881 tgcaatttat tgaattttag ttgtttttag gccggataag gcgtttatga actgaccccc
     8941 aaaacctgga cagtttcagt taaccggctt tttgatactc agtcctgtat tcagggcaag
     9001 attacgaaag cccgctcccc gcaaggactg acgccagata gtttctgtcc atggctgctt
     9061 ttcgcatctt acgtcttaac cctgccttga ataccttatc attcgtcaaa atattaatag
     9121 caatgtgccg tatccctgaa aataattctg ctgcatttcc tcttcttatt ttgcagtcgt
     9181 cttcattcat taccacgtcc agacgccagt gcagcttatt ctccacgtgc cagtgatttc
     9241 ggatcgctgt ggcgaatttc tcagcggtta aatcagcaga actgatataa tatctgaccg
     9301 tcatttctgg ctctttcttt tgttctgcta ttatggaccg aaaggagact gccacgcata
     9361 atttcttcag ccctttccat tcaaacgtga aatcaataag ttcatcaggg acatcgcaaa
     9421 caatatgaag acggatttct tctctgccgt gactcttttc actgattgcg taactgtcat
     9481 gctctggatt atttaattct ttcagcggaa atttttcctc aaaggcttta tttagccgcc
     9541 cctgggttcc ttttaccgcg aataaataat cacctccctg tttttgtatc ttctctgcaa
     9601 tatctttctg gcaacccatc gcatcagttg tgatgatttt tcctttaata tccagcatgt
     9661 taagaagttc tgggatagct gtaatctcat tagatttctc atccgtcttg atctgtccga
     9721 tgaccagact gtgcattgtt gagaacgcac taatgacatg aatcgctccc ctgcggcgac
     9781 tcttgtcata agagtgccgg agcgtttttc catcaattgc aatgacgtct ttatcatctg
     9841 aagaatggca gtcacgcatc cagttaataa agcactcgtg aaattttgca ggactgatac
     9901 aggatacaac tctggcaatg gtatcgtgaa caggaatacc attttcaaaa tcaccatatt
     9961 gcttcaaaaa atcgagatgt gtttccccaa aatcctctat atcttcccaa ccttctgcac
    10021 cagaaataac ggcacaaata gtcaacagta gaatatccga taatttatgt tccactttcc
    10081 aggtttgtct gtaatcgggg ataatagaaa tatgttccat caatttttta agttccattt
    10141 tgttctcctt aattaggtaa ggagtatttg atcatgtata agcaataaaa aacagcttca
    10201 ggtaataagg aatatctcaa tttttaaaca taaaatgcga attatttagt gcaaaaagtg
    10261 aacttttgat gatctttccc tggccgttga ctcgattgcc gtagctcgaa cgcaagcact
    10321 cttatcattc aatgccatat tgttcaaaat attaaaaaca ttatcttcgc attttttgca
    10381 aatttatatt tgcccgagta taaatgctcc gatatctcta cggatataat ttttgtcagt
    10441 gcagaattca attgccaatc taacagcgtc ttgcccccct cttaactgta atactctggc
    10501 agatgaaatc cttttcaagg aattgcgatc atcaagtaag cgaaataatt catcatcatt
    10561 tagtttctta catttattat ataaaccata ctctttactt gcctttcttt tctggtatgt
    10621 atttgacatt gcaggaacct ccataaaggc tatctttttc tatcgtttga ctgtctggag
    10681 tcctccacct actctgccat cagcgcacac gatgtttgat tatccttaca tacttatttt
    10741 ccgtggccaa aattcagaat tctcccatca tttttgccta tttctataat aaaaacccca
    10801 ccagcaattt ggtctgagtg aatggtacct tcaacaaccc aacttgttgt taactcagtg
    10861 atttcgtatg gtttttcttc ttctgcggca tcctgtccat aacgattctt aacatatatc
    10921 tcagctaatt ccaatgctat agctttattt ttaaccaagt ttctaacggt atatttacct
    10981 tcactttcat acttgcgcat tattttaata ttattattac actgcgccca tgtgttgtat
    11041 gtaaaaacaa acaaagatac aatgacacag ttcctaaaca atattttttt acatattacc
    11101 atgaactctg cagacattag gtaatccttt tctgtttgtt atttccttcc cgtggttatc
    11161 tattgcttta aaattaccac tgggcgtacc taaataaaat gacttgatat tgttatcttt
    11221 acttttaact attttctcat ctttacctga aaatatttcg tcccaatatt ctccatgact
    11281 atctgcacca tgagtatgat aagctgatac tttctcagta ccattagggc aagggctatt
    11341 gaatggatat gatccttttc tttctcctcg gttaggtgct gttgagaaat atctgccact
    11401 attatcctta catattaaac cagcgaactc tttatcttcg caaatagatt ggctatttat
    11461 tttatttaag gcgtcatatg cggtgctatc tatagaatca aatttatctg gacctatcat
    11521 agcggatagt gtgccgcaaa gaccattagt acatgccccc gattttgcat cctcccacat
    11581 agataaaccc agggggtcga tgtctgatat cggattcaat tgatatccat acaggttcca
    11641 tccccccttc agtccaatcg gatcctgagt gatatatcgc ccctgcagcg ggtcataata
    11701 gcggtggcgg ttgtaataca ggccggactc ctcatcatac tgctgccccg gcagccggat
    11761 aagctgctgc agctgatgcg ggttctcttc attcagcagg ttgccccatt catcatattc
    11821 tgcgcaccac gctgttgccc cttccgtgct gatgag
//
LOCUS       DBBGLQ010000126        11474 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.126, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000126 DBBGLQ010000000
VERSION     DBBGLQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.126"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..1093)
                     /gene="allB"
                     /locus_tag="ACIYSB_004528"
     CDS             complement(<1..1093)
                     /gene="allB"
                     /locus_tag="ACIYSB_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HGC5431434.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLM"
     gene            complement(1153..2607)
                     /gene="ybbW"
                     /locus_tag="ACIYSB_004529"
     CDS             complement(1153..2607)
                     /gene="ybbW"
                     /locus_tag="ACIYSB_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGC5431435.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQNQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(2776..3654)
                     /gene="glxR"
                     /locus_tag="ACIYSB_004530"
     CDS             complement(2776..3654)
                     /gene="glxR"
                     /locus_tag="ACIYSB_004530"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGC5431436.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(3754..4530)
                     /gene="hyi"
                     /locus_tag="ACIYSB_004531"
     CDS             complement(3754..4530)
                     /gene="hyi"
                     /locus_tag="ACIYSB_004531"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGC5431437.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKHVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(4543..6324)
                     /gene="gcl"
                     /locus_tag="ACIYSB_004532"
     CDS             complement(4543..6324)
                     /gene="gcl"
                     /locus_tag="ACIYSB_004532"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGC5431438.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(6414..7229)
                     /gene="allR"
                     /locus_tag="ACIYSB_004533"
     CDS             complement(6414..7229)
                     /gene="allR"
                     /locus_tag="ACIYSB_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGC5431439.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(7307..7789)
                     /gene="allA"
                     /locus_tag="ACIYSB_004534"
     CDS             complement(7307..7789)
                     /gene="allA"
                     /locus_tag="ACIYSB_004534"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGC5431440.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHCNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            8019..8945
                     /gene="allS"
                     /locus_tag="ACIYSB_004535"
     CDS             8019..8945
                     /gene="allS"
                     /locus_tag="ACIYSB_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGC5431441.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            9014..10108
                     /gene="mnmH"
                     /locus_tag="ACIYSB_004536"
     CDS             9014..10108
                     /gene="mnmH"
                     /locus_tag="ACIYSB_004536"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGC5431442.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEHGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLREQMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            10224..10589
                     /gene="ylbG"
                     /locus_tag="ACIYSB_004537"
                     /pseudo
     CDS             10224..10589
                     /gene="ylbG"
                     /locus_tag="ACIYSB_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(11110..11283)
                     /locus_tag="ACIYSB_004538"
     CDS             complement(11110..11283)
                     /locus_tag="ACIYSB_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431443.1"
                     /translation="MAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFI
                     TNIGAGLPDACAE"
     gene            complement(11340..>11474)
                     /locus_tag="ACIYSB_004539"
     CDS             complement(11340..>11474)
                     /locus_tag="ACIYSB_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431444.1"
                     /translation="YINYMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFLIFP
                     "
BASE COUNT         3014 a         2989 c         2647 g         2824 t
ORIGIN      
        1 ccattaattt gccgaacatt ggcagagaca taccgcgttt ctgtaccgct tcatcgaaca
       61 tcacgtccat gcagctttgc agaccggcga taccgcccca tgctttcatg atgttaccgg
      121 ctttcatttc cggcgggcat ggagagtggt cggaaaccag gcagtcgatt tcaccgttaa
      181 acagtttttc ccacatgcct ttctggtttt ccagatcgcg gatcggcggt gaacacttcg
      241 ccagagtacc gatttcttcg aactgatcgg tatccagtac aaagtaatgc gggcaggatt
      301 cacaagtaac gtcctgacct tcctgacgtg cacgagtcac ttcctcaaca ccttccgggc
      361 tgctgacgtg gcaaacgtgc agacggcaac cagcaacttt cgccagatac agtacgcggc
      421 gaattgcttc cacttcggta aataccggac gcgaagccac atagtcatga gcggttacgc
      481 gaccttcacg cttcgcttct tcgcccagtt cgtcacaaat cagcgcgttt tcgcagtgca
      541 ccagcaccgg ctgacccagt tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt
      601 ttacatcacg gaagtcgttg tcgataccgc gatcgccaca ggtcgcaacg aagcatttga
      661 agccgacaac gcccacttca tccagctcat gcagacggtc gatgttgtaa gacaccaggc
      721 caccgagttg tgccgcatca atagtcagct tgcctttagc ggcatcgaac ttcagttcaa
      781 ttgaagcgcg gtcaaccgtt gcaggcagct ggttgagcgg catttcgatc atggtggtga
      841 taccaccttt tgccgctgcg cgagtaccgg tttcataacc ttcccagtgg ctacgacccg
      901 gttcagaaat atgggtgtgc gcatcaacca tgcccggcga aaccaccaga ccagacgcat
      961 ccataacttc ttttgcatcg cccagatcct gaccgatagc agcaattttt ccgcctttaa
     1021 cggcgatatc tacaacgcga gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta
     1081 aatcaaaaga cataacaaac tccttgtaaa atacagattg gtttttaact caatgttcaa
     1141 tatcgggatt aattaaccta tggttttttg ctctcctgtt ttttctgctg ttgtacgttt
     1201 ctttaataag gcgtaggccg caaaggcgac gatgacgccg acaaaccatg aaacacgcga
     1261 taacggttcc ataaagtgaa taaacttacc gccaagagat aaaataacgg ccaccagagt
     1321 tactgaaaac gcagtgaggt taaaaccgtt atcgtaatat ttataatcgc caggtgcggt
     1381 atacagttca tcaagattaa tttgtccgcg catcaccaca aaataatgcg ccatcatgac
     1441 accaattacc ggaccaagca ttccgccgat aatatcgagg aaaagataaa tgctgtcctg
     1501 attttccatt aatttccacg ggcagatcag caagctgata atactggcaa tcagtacgcc
     1561 gtttttatag gtcagttttg tcggtgcaat ggcggcaatc tgataaccgg ctggaataat
     1621 attaccggtc gcgttagtgg agatagttgt catcagaata accagtaccg caaagaacga
     1681 ggcgaacagg ctgtcccaac gctgaacaat atccagcacg ttccaggtat cagcgccgta
     1741 gtgaatactg gctccggcaa taatacatac cccggcgacc gcaaacagaa tataggccac
     1801 aactaaaccc agcgtttgcc ccagcgcctg ctcacgaaac gagtgggcgt tttgcgtaaa
     1861 gtcggatgcg ctcaccgccg gtgccgccca gaccgcaact accgcgttaa tcaccaccag
     1921 gaacaggaag ccaccgtttt ctgctttctg aataccgctc ggaatgtagt caaagattgg
     1981 accgatcccg accagtgaaa tcgcccaaat cgccataccg ccgaaaacga tatagatgca
     2041 cgggttaaga atggcagtga atttatttaa aactttgcca ccgccaaaac ctataccaac
     2101 gttgaccagc cagaagatta agaaagtaat taagcccggt agagaaaggc ctaacagagt
     2161 gaaatcacca ccgagagtta aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc
     2221 cagtgacccc gcgtaacatt gcaaaccaaa ccacatgatg gcggcaattc cgccccttaa
     2281 taatccggga aacagtgcac cacgtacacc gtaagaagca cgcaggatca tggcaaaagg
     2341 cacaccgtat ttactgcccg cagcaccgtt taataccatt accgcggcaa tgaaaaaggc
     2401 gctgaggata attgccagca taatactaaa ggtagacaag ccgagaataa aaaagccgcc
     2461 gaccatcaca taattgggaa cgttatgaac cgaacccatc cataaggtaa aatagttaaa
     2521 tgttttccag gtccgctggt tttgcgtttt cggcaataga tcttcgctat agccgcgttg
     2581 ctggaatagt tttctctgat gttccataaa tccctcttaa tttcacgtat cgaataacgt
     2641 tattttaatc tagctatgag gaacaaataa aataaaaata ttatttttaa tactgaagca
     2701 acatctttgg ctgctttctt ttttaatatt gctggccgat tcattttctg atatcggcca
     2761 tttttattgc gggtatcagg ccagtttatg gttagccatt aattccagcg cctgcactaa
     2821 cgcagagtga tccaactggc tgccaccgtt tgccgcacag gtattaaata actcctggca
     2881 ggtcgcagtg tttggcaggt tcagcgcaag tgctttcgca ctttgcagtg ccaggttgag
     2941 atctttctgg tgcagagcga ttttgaagcc cggattaaag gtgcgtttaa tcatacgctc
     3001 gccatgaact tccagaatac gtgaggaagc aaagccgccc atcagcgcct ggcgcacacg
     3061 taccgggtcc gcaccggctt ttgaagcaaa tagcagggct tcagaaaccg cttcaatatt
     3121 gagcgccacg ataatctgat ttgccacttt gcaggtttga ccatcgccgt taccgcccac
     3181 gagggtgata tttttaccga gcagttcaaa cagcggttta acacgttcaa ataccgcttc
     3241 atcaccgcca accataatcg acaacgtccc ttcacgcgca ccgatttcac cgccggagac
     3301 tggcgcatcg agataatcgc cgcccagttc attcacctga cgagcgaaac gcttagtttc
     3361 aatcggggaa atggagctca tatcaacaat ggttttgccc ttcagcgagg ctttggtaca
     3421 accattttca ccgaacagaa cttcttcaac ctgaggtgtg tccggcacca taataaaaat
     3481 gatgtccgat gcttccgtta cctggcgagc agtttcaaca ctgacggcac ccagtgacag
     3541 taattcatca gcaaccggtc caatggtcgt gacatgtaat tgatgaccgg cacgcgccag
     3601 attaatggcc atcggtgtac ccataatgcc taagccaata aatcccagtt tcataattaa
     3661 cctcttttaa atttcgcttt tcctgaattc agacaacacg atcccctgcc aacttagcgg
     3721 cctaaaaaag cattgtctga atagcgttac gttttaacgg tacggatcca tccagcgtaa
     3781 accggcttcc gtggtggttt ggggtttata ttcacaccca acccaaccgt tgtagtcaga
     3841 attttcgatt accttaaaga gataatcata attaatttct ccggttcccg gttcgccgcg
     3901 atgcggatta tcggcaattt gcaggtgacc aattttatca gcccactgag tcatggtgtt
     3961 ggttaattca ccttccatcc gctgcatatg ataaatgtca tactgaattt tcaaattgca
     4021 gcaaccaaca tcatcaatca atttcagcgc ctgccgagtt ccggtgagat ggaaaccagg
     4081 aatatcaaaa tggttaatag gttcaatcag taataaaata tcttctttca tcagcatatt
     4141 cgcggcataa cgcaggtttt ctacaagcgt tgcgtgaatc tgttcactgc tgaaaccagc
     4201 cggcgtttta ccgaccagac agttaatttt tttattaccc agcgcacggg cataacgaat
     4261 cgctgctgct acgccatccc gaaactcttc ttcacggcca ggaatacagg caataccgcg
     4321 ctcccccgcc gcccagtcac ccgccggtaa attgtgcagc gtatgttcga gtttattact
     4381 cgccagcaca tgttttaatt cttcaatgtc gtagtcataa ggaaacataa attcaacgcc
     4441 gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga aaatcatatt ctccaaataa
     4501 catcgataaa ttagcagaga aacgtaacat tatttatctc ccttattcat agtgcatgaa
     4561 gcaggtttca gtcggtgcgt ccgctgcgtt atcggcgata tcttcaaatt ccatgacgtt
     4621 atccagttcg ctgcccatcg aaatattggt cacacgctcg agaataactt ccacgactac
     4681 cggtacccga tattgcgcca ttaaggcttt cgcctgttca aaggctggcg caatatcttc
     4741 cggtttgaag acccgaatag ctttacaacc taaaccttcc gctactttta cgtggtcaac
     4801 accgtagcca ttcacttcac tggagttgat attctcgaaa gcgagttgca cgcagtagtc
     4861 catgtcaaaa gcgcgttgtg actgacgaat caggccgaga taggcgttat tcaccagcac
     4921 atggatgtac ggaatgttga actgcgcgcc aacggctaac tcttcaatca ggaactggaa
     4981 gtcaaagtcg ccagaaatcg ccaccacatt gcgtttcgga tcagcggcac aaacccccag
     5041 cgccgccgga atcgtccagc ctaacggacc agcctgacca cagttgatcc agtggcggtc
     5101 tttaaagaca tgcagcattt gtgccgcagc gatttgtgac agaccaatgg tggtgacata
     5161 gcaaacatcg cgaccaaagg ctttgttcat ctcttcatac acgcgctgcg gtttcaccgg
     5221 cacgttgtcg aaatgggttt tgcgcagcaa agtgcgtttg cgctgctggc agtcggcgac
     5281 ccattcttta cgacacggca gacgacccgc tttttgcatc tcctgcgcca cttcaaccag
     5341 cagtgtcagc gccgctttag catcagagac aatgccgaga tccggacaca gcacgcgacc
     5401 aatttgcgtc ggctcaatat cgatatgaac gattttgcgc ccttcggtgt atttctctac
     5461 cgaaccggta tgacggttag caaaacggtt accgatacca aacaccatgt cggacgccag
     5521 caacgttgcg ttaccgtaac gatgcgcggt ttgcagaccc accatcccgg ccatcagttc
     5581 atgatcgtcc gggatacagc cccagcccat cagcgttggg atcaccggaa cgctggtcag
     5641 ttcagcaaac tgttgtaaca gtgcagctgc gtcagcatta attaccccgc ccccggcaac
     5701 aatcaccgga cgttcggcct ggattaacat ttctacagct ttttcgatct gcatacggct
     5761 ggcagcaggt ttgtagaccg gcagcggttc gtacatgtca ggatcaaact cgatttccgc
     5821 aacctgaacg tcgaacggta aatccaccag taccggaccc ggacgaccag aacgcatcag
     5881 gtgaaatgcc tgttgcagca cgcgaggcac cagcgccgct tcacgaactg taaccgccat
     5941 tttgctgacc ggtttagcaa ttgcttcaat atctacggcc tgaaaatctt ctttatgcag
     6001 acgggcgcgc ggtgcctggc cggtaatgca cagaatagga atggaatcag cagaagcgga
     6061 atagagcgcg gtgatcatgt ccgtgcccgc aggaccggaa gtccccagac atacgccgat
     6121 atttcctgcc gttgcgcggg tataaccttc cgccatgtgc gaagcacctt ccacatgacg
     6181 cgccagaatg tgacgaatac cgccgtgctt acgcatcgct gagtagaacg gattgattgc
     6241 agctcccgga acaccgaagg cggtggtgat accttctttc tccagcacat acattgccgc
     6301 gtcaacggct ctcatttttg ccattttatt cctacctcta tttattggaa aatttttcca
     6361 actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg gatgcgacag acattatgga
     6421 tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg tgtctctgac cagctcaccc
     6481 tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc cggagatgga gatagcggca
     6541 acaacactac cgacatcatc gtaaattgct gaagctatgc aattcagacc tacaacatgc
     6601 tcttctttat ctacggtata gcccagttca cgcgcttgtt ccaggtcctt cagcaaggtg
     6661 ggcatatcca caagcgtagt tggcgtaaac tgctgcaaac cggtttgcag aatgatgctc
     6721 atcaactcct cttccgccag cggataaagc agcgctttgc ccgcaccgga agcatgcagt
     6781 ggcagacgac tgcccagtgg cgcacacatc ctgaccatcg atttacactc taactgacca
     6841 attaataccg cttcattgcc gttacggatc gcgacattga ccgtttcgcc ggaaagtaac
     6901 atcaggcggc gcataaacgg cccggcgacg gagaggacat cgcggttatg gatgtacgcc
     6961 gcaccgacgt taaagacacc taatcctata tgccaccagc ctaattgact gtcctgatag
     7021 acaaaatccg ctgcctgtaa aaccttcagc aagcgaaagg tcgtggagag cggcaaatcc
     7081 agattgagag aaatatcgct aaccgacgaa cttcccccac ttttttccaa atattgcaga
     7141 atcgcaattc cccgctctaa cgcctgtgcg cccttctgtg ccacaggctc cgcctgtcct
     7201 ggcctgccgc gccgtctaac ttccgtcata cctacgttaa ctccgaccta caagtgagca
     7261 taaacaatga acaattagtt tatcttatgc aagtcggttg caggcgtcac gcaaaacaga
     7321 gttcctgttc aggaatactt tcaacatcac agttgtcact gccgccgcga tcgatggtca
     7381 gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg ccagacgtta caatggtaat
     7441 tcactccctg ttcgccgttg gtgataaacg cccgcagcgt tgacaggtct ggtttgtcgt
     7501 cacctaacgc cacgaccacc acaaacacct cacctttcat cgggataaag gcctgagtac
     7561 ccagcggatg acgttcgagt tcgtgaatgg tcagcggcag attcgccggt tgcgcgcggt
     7621 taatgctgat aagcgtacgg tcttgctcaa gaatctcaac cagcgccaaa tcgtggtaac
     7681 gctccaccag gccattgtta atatggaaaa aatcccgttg ctgcgtttcg attacgtcgc
     7741 cataagcact aaaggcttcc tgacttaacg gtaatacctg aagtttcatc tttttgtcct
     7801 tgtaacataa ttccgttcaa ccaccgccga ccggtcagct tggtttcaat aacaggcaat
     7861 ctaaaacctt aagagaacaa catcaataca aaagttggaa tatttttcca aaactagatt
     7921 tagtccacaa attgaacagg gttatcatgt aaaaatactg ataaatcaga ggataagaaa
     7981 atcacgcaac ttgcggattt aaaaggggat acctgaagat gttcgatcca gaaaccttgc
     8041 ggactttcat tgcggttgct gaaacaggaa gtttttcaaa agcggcagaa cgattatgta
     8101 aaaccacggc gacgatcagt tatcgcatta aacttctgga agagaatacc ggagtagcgc
     8161 tgtttttccg tacgactcgc agcgtgacgt tgacagcggc tggcgagcat ctactttccc
     8221 aggccagaga ctggctgagc tggctggaaa gtatgccaag cgagctgcaa caggtgaatg
     8281 atggcgtgga acgccaggtg aatattgtca tcaacaacct gctctacaac ccccaggccg
     8341 tcgcccagtt gctggcgtgg ctgaatgagc gttacccctt tacccagttt cacatctccc
     8401 gacaaatcta tatgggcgtc tgggactcgc tattgtacga aggtttttcg ctggctatcg
     8461 gcgtcacggg aactgaggcg ctggcaaata cctttagtct tgatccctta ggatcggtgc
     8521 aatggcgctt tgtcatggcg gcggatcatc cgctggcgaa cgttgaagag ccgctaacag
     8581 aagcgcagtt gcggcgcttt ccggcggtca atattgaaga cagcgcccgc accttaacca
     8641 aacgcgtcgc ctggcgattg ccagggcaaa aagagattat tgttcccgat atggaaacga
     8701 aaatcgccgc ccatctggcg ggcgttggca ttggtttttt gccaaaatcg ctttgccagt
     8761 caatgatcga taatcaacaa ctggtcagcc gggtaatccc aacgatgcgc cctccttcgc
     8821 cattgagtct ggcatggcgc aaatttggca gcggcaaagc ggtagaagat attgtgacct
     8881 tgtttaccca gcgcaggccg gaaatcagcg gatttttaga aattttcggc aacccacgca
     8941 gttaaaaatc aagtttcctt gcgcgttggc gcgtaaaata gcgcgcaagc tgatttgagg
     9001 ttcactttta actatgcaag agagacacac ggaacaggac tatcgtgccc tgctgattgc
     9061 tgatacgccc attattgatg ttcgcgcccc tatcgagttt gagcacggcg caatgcccgc
     9121 cgctatcaat ctgccgttaa tgaataacga tgaacgcgcc gccgttggca cctgctataa
     9181 acagcaaggc tcagacgcag cgctggcgct gggacataaa ctggtggcgg gtgaaattcg
     9241 tcagcagcgc atggacgcct ggcgggcagc gtgcctgcaa aatccgcaag gtattctctg
     9301 ctgcgcccgt ggcggtcagc gctcacatat tgtgcaaagc tggttgcatg cagcggggat
     9361 tgattatccg ctggtggaag gcggttataa ggcactgcgc cagaccgcga ttcaggcgac
     9421 tattgaactg gcacaaaaac cgatagtgct gattggcggt tgtaccggca gcggtaaaac
     9481 gctgttagtg cagcaacagc cgaacggtgt tgatctggaa gggttggcgc gtcatcgcgg
     9541 ttcggcgttt ggtcgcacgt tacaaccaca acttagccag gcgagttttg aaaacctgct
     9601 ggctgccgaa atgctaaaaa ccgacgcccg tcagaatttg cgcctgtggg tgctggaaga
     9661 cgaaagccgg atgatcggtt cgaatcacct gccggaatgc ctgcgcgagc aaatgactca
     9721 ggcggcgatt gcggtggtag aagatccgtt tgagatccgt cttgagcgcc tgaacgaaga
     9781 gtatttcttg cgtatgcatc atgattttac ccacgcgtac ggcgacgaac agggctggca
     9841 ggagtattgc gaatacctgc atcacggact ttcggcgatt aagcgtcggc tggggctaca
     9901 gcgctataac gaactggctg caaggctgga tgcagcactg acaacgcaac tcaccaccgg
     9961 cagcaccgac ggtcatctgg cctggctggt gccgttactt gaagaatatt acgacccgat
    10021 gtatcgctat cagcttgaga aaaaagcgga aaaagttgtc tttcgcggtg agtgggcgga
    10081 agtggcggaa tgggttaagg cgcggtaatg agggacgtat ttgtattagt cgcgacttga
    10141 tctgacacat ggccttgaaa ggttgagagt taccggtttt gatatgagtg tcgaatcctt
    10201 atccaaaaca agaggtaact ctcatgcttc atactgccaa tcccgtcatc aaacacaaag
    10261 ccggtttgct caatctggct gaagaactca gcaacgtatc aaaagcctgt aaaatcatgg
    10321 gcgtttcgcg tgatacgttt tatcgttacc gcgaactggt cgctgaaggc ggtgtggatg
    10381 cgcagattaa tcgtagtcgc cgcgctccta accttaagaa ccgtaccgat gaggcaactg
    10441 aacaggctgt tgttgattac gccgtcgctt tcccgacaca tggtcagcac cgggccagca
    10501 acgagctgcg taaacagggg gtttttatct ccgacagtgg tgtccgttct gtctggttac
    10561 tccataacct tgagaatctc aaaagacgat actgaacaaa ttttatcagg tgacgttccg
    10621 taaaaagttg tatggagttc tcgatgcagt cggcgcgact atcagtaccc ccgctgaacc
    10681 ctggactatc gggccgccgt actggggcat gtcaccctgg cgtgccgctg gttttccgct
    10741 aatggtttat ttcctctttc tttcccggca agaatttatt ttaatccgga aggattattt
    10801 attttcagat agggactcac attaaacacg taacatttta attaatgcat ggctgaatat
    10861 gtattacaca atgtcagata tcgtaaaaga ccagtgcaat tctatgtaaa ctctctgact
    10921 gttcatttta tttgttgttt cagggtcgga actcaccctc aataaacaac tgaatacgag
    10981 tagatattca tcgtctgagc tatatggctt tacacaatag ccgacatgtc ttttctcagt
    11041 caggaatttt acttttcctc actacgaagt tgacgacttc gatatgggat agactcttaa
    11101 ttcaagcaat tactctgcac aagcatcagg caatccagct ccaatatttg ttataaaata
    11161 atgcgtaaca ttaatttcac caacaaaaaa aaggtttaca ccatcccctt gccctggtat
    11221 cttaccatat ttacaccaca ctttttttgt atctggatca ttctttgata cgattttcgc
    11281 cacttgttcc ctggccctca ctgtcaatgt tttcttaaac ctacccccat catcatttgt
    11341 tagggaaaaa taagaaaaaa ataatcatct tctacatttg tgtatattga tatatccacc
    11401 gctacgtgcg gaaaaaaatc tgggagccat gtaaaaactc catcattttc tggtattgcc
    11461 atatagttta tgta
//
LOCUS       DBBGLQ010000127        11462 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.127, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000127 DBBGLQ010000000
VERSION     DBBGLQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.127"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..294
                     /locus_tag="ACIYSB_004540"
     CDS             <1..294
                     /locus_tag="ACIYSB_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HGC5431445.1"
                     /translation="DRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLK
                     NESLSHYRFNSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            448..2445
                     /locus_tag="ACIYSB_004541"
     CDS             448..2445
                     /locus_tag="ACIYSB_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HGC5431446.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2508..3785
                     /locus_tag="ACIYSB_004542"
     CDS             2508..3785
                     /locus_tag="ACIYSB_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HGC5431447.1"
                     /translation="MNSRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
     gene            4033..4689
                     /locus_tag="ACIYSB_004543"
     CDS             4033..4689
                     /locus_tag="ACIYSB_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HGC5431448.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYFKSKEDYAQTVLNAYDSFFEHNLKKHLHESSCTPMVRLENFI
                     NDACEGIKKYNFTRGCLVGNMMQESPGLPQSFIKVLQNILESWQALVAACLSDALSSG
                     EISSNMNNTQLAAIFWSGWEGAVMRSKLYCSTEPVYDFWSYFKTSVRYQSSQEATTGQ
                     "
     gene            complement(4870..4977)
                     /locus_tag="ACIYSB_004544"
     CDS             complement(4870..4977)
                     /locus_tag="ACIYSB_004544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGC5431449.1"
                     /translation="MGNMAPFILRGVTLKGIDSVMFAKEKRAAIWQQAV"
     gene            5969..6195
                     /locus_tag="ACIYSB_004545"
                     /pseudo
     CDS             5969..6195
                     /locus_tag="ACIYSB_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(6197..6979)
                     /locus_tag="ACIYSB_004546"
     CDS             complement(6197..6979)
                     /locus_tag="ACIYSB_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HGC5431450.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSIPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            7285..8205
                     /gene="rbsK"
                     /locus_tag="ACIYSB_004547"
     CDS             7285..8205
                     /gene="rbsK"
                     /locus_tag="ACIYSB_004547"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGC5431451.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            8233..9549
                     /gene="fucP"
                     /locus_tag="ACIYSB_004548"
     CDS             8233..9549
                     /gene="fucP"
                     /locus_tag="ACIYSB_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGC5431452.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNQHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRNALAEVTES"
     gene            9561..10574
                     /locus_tag="ACIYSB_004549"
     CDS             9561..10574
                     /locus_tag="ACIYSB_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HGC5431453.1"
                     /translation="MSTRIYLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHSLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(10800..11462)
                     /locus_tag="ACIYSB_004550"
                     /pseudo
     CDS             complement(10800..11462)
                     /locus_tag="ACIYSB_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2933 a         2568 c         2747 g         3214 t
ORIGIN      
        1 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtttggtctg
       61 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      121 ggaacgctga aaaatgagag cctgagccac tatcgtttta atagccggga tgaagccatc
      181 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
      241 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
      301 aacaaatggt agtgtccgct attgccagta cacctcaaca ttccaccatg cattccgatt
      361 aacgccgcat agccagttga actttgctac tttgtgagag gtagtacctt ctatccagtg
      421 cgaatttaat taatggaata aatgattatg agtgaaaatg atacaatccc aaagaagtct
      481 acaagtcaga ttaacaaagc ggtattcttt acatctgctt tgctaatttt ccttcttgtc
      541 gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt ttaaactact tcagcaacaa
      601 atcttcacga atgccagctg gttctacatc cttgctgtgg ccctgatttt actgagtgtc
      661 acgttccttg gactctcacg ctacggtgat atcaagctgg gcccggacca tgcgcagcct
      721 gatttcagct accactcctg gtttgcgatg cttttttcgg cagggatggg gatcggcctg
      781 atgttctttg gcgttgccga acctgtaatg cattatcttt cgccacccgt tggcactcca
      841 gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct ttttccactg gggactgcac
      901 gcatgggcaa tttatgccat tgtggcgctg attctggcgt tcttcagtta ccgtcacggt
      961 ctgcctttaa ctctgcgctc cgcactctat cccattattg gcgatcgcat atacggacct
     1021 gtaggacatg cggttgatat tttcgctgtt ataggcacgg tctttggcgt tgcgacatca
     1081 ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc atcttttcgg ggtgcccatc
     1141 aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg ggttagcgac gatttcagtg
     1201 gtgtccggtc tggataaggg aatacgtatc ctgtctgaac tcaatctggg tctggctttg
     1261 ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc ttctgctgaa gtcatttgtg
     1321 gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa cgttcaacct ttacgcgtat
     1381 gagcccaagt cgagcaactg gctggggggc tggacattac tgtactgggg atggtggctt
     1441 tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct cccgcgggcg aaccattcgc
     1501 gagtttgtca ccggcgtgct gtttgttccc gcgggtttta cgctaatgtg gatgacggtg
     1561 tttggtaaca gcgcgatcta tctcattatg aaccaggggg ccacagacct cgccaatact
     1621 gttcagcagg atgtggcgct ggccctgttt aatttcctgg agcatttccc gttctcttct
     1681 gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct ttgtaacgtc tgctgattcg
     1741 ggggcaatgg ttgtggatac tctggcatca ggtggagtgg caaacacacc cgtctggcag
     1801 cgaatattct gggcctcgct catgggcatt gttgcaattg cgcttctcct tgccggaggg
     1861 ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc ccttctcagt gatcttacta
     1921 atatctatat acggactttt aaaagctttg cgccgggatt tgaccaagcg tgaaagcctg
     1981 agcatggcga caattgctcc tacggctgca cgtaacccaa ttccttggca gagaaggtta
     2041 cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac gttttatgga cgacgttatc
     2101 cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc aggggacgat aagccacatt
     2161 agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt tgggcaacga gctgaatttt
     2221 atatatgaag tgaggcttcg cgggtatatc tcaccgacct tcgcgctcgc cgcaatggat
     2281 aatgatgagc agcagactga acaacatcga tattatcgcg ctgaggttta tctcaaagaa
     2341 ggcggtcaaa attatgatgt gatgggctgg aaccaggaac agctgattaa tgacatactg
     2401 gaccagtacg aaaaacacct gcacttcctg cacctggttc gttaatagca acatgccgtc
     2461 ctgggggcgg caattattat cccggccgca atatgaggga atgcagaatg aattcacgct
     2521 ggaaatggat gctgaagcag acaattaaaa aactatggtt cagggcaacg ttattcgcaa
     2581 ttgtcgcgat aataacggcc cttttatcaa ttctttttaa atcaatgata cctgagtcgg
     2641 tttccgtgaa ggttggtgcg gaagcagtcg ataacattct gaacatactg gcatcgagta
     2701 tgctggcagt gaccacattt tcgctgagta tcatggtcac agcctacggt tcagccacta
     2761 ctaatgtgac tcccagagct acgcgtttag ttgttgaaga cgtcaccaca caaaatgtac
     2821 tggccacctt catcggttct tttctcttca gtctggtagg gattattgcc ctcaatatgg
     2881 gagcttatgg agaaaggggg agagtcattt tattcattgt cacactggtt gtcattgcct
     2941 taatcctcat cacattgctt cgctggatac agcatttgac ctctctgggg agggttggtg
     3001 agacaacggc acaagtagaa caggcggcca tcgaaacatt tattgcgaga gcaagaaatc
     3061 cctgtctcgg cggatatcca tggcttgaga acaatgaaca gccgaaagga acggttgcag
     3121 tttatccgaa gaagattggc tatgttgaat atattgatat ggtgaaactt agcaagctgc
     3181 tgaccaatga tccccgtcat gtatacctcg tggcgcagcc aggcagtttc atacatccgt
     3241 ccatgccagt tttgtacctg agtcagggcc aggagtcgtc aatcaacacc gatttacttg
     3301 agacgattat tgtctcggat gcacgttcat ttgctcagga tcctcgattt tgtcttagcg
     3361 tcatggccga aatagcctgc cgggcccttt cccctgcagt gaacgatcct ggaaccgcca
     3421 ttgatgtcat tggcagaggt gttcgtatac tttccactta cgcgcagaat aaatctgatg
     3481 aaatagaagt gaaatatcct tcagtacatg ttgcaccact tcagaataac gatctactgg
     3541 aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat gagggagatt cagataagag
     3601 tccttaaagg actgtcgatg ctgagtaaag gttggcctga gatatttgct gaagccgcac
     3661 ataccctggc atttgaaaca ttagagcatg caactcgtgc tgaccatata gattctgata
     3721 gatatctaat aaaatcaact tattataatt tatttagtgg cgaatattct aataaaaaaa
     3781 catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa gggcaaagca ttcgcgtgag
     3841 aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat tctgcactgt tcagcgatgg
     3901 cgcagtagag acaggcactg ccactgtcca gtcggcaaaa gcgaacactt tgccactgcg
     3961 gggataaact gattgaagct ctcgcgcaaa atcacttatt cttttgcgtt aataaaatgt
     4021 aggagatggg tcatgacagg cgaaaatcgt ggccggggaa ggccgcgaaa gacagaaagt
     4081 acctatgcag atactcgtaa tgatctgatc cgcagtggcc ttgaactgct gacgcagaat
     4141 ggttttcttg cgacgggagt cgatgccatt gttaaaaatg ccaatgtccc taaaggctca
     4201 ttttattact actttaagag taaagaggat tatgctcaga ccgttctgaa cgcctatgac
     4261 agtttcttcg agcataacct caaaaaacat ctacatgaat catcctgcac accaatggta
     4321 cgtctggaaa atttcatcaa tgatgcctgt gaggggatta aaaaatacaa ttttacccgc
     4381 ggctgcctgg ttggaaatat gatgcaggag agtccggggc ttccccaatc cttcattaaa
     4441 gtactacaga atattcttga gagctggcag gcacttgttg cggcctgcct gtccgacgcg
     4501 ttatcttcag gcgaaataag ttcaaatatg aataatacac aactggcggc tattttctgg
     4561 agtggctggg aaggtgcggt aatgcgttcc aaactatatt gttctactga gccagtgtac
     4621 gacttctgga gctatttcaa aacctcggta cgctatcagt cttctcagga agcaacaact
     4681 ggccagtaaa aggggccgca gcccctaaaa cttattatac taagcctgag ctcggcatcc
     4741 atctaattag ctatattgaa tgactgcacg accgcgaatt ccgctactca gaagtttttc
     4801 agccacagtg atggcgtcac tgagcggata aacggtagtg agactatcca ttctggactc
     4861 tggcaaatac tataccgcct gttgccagat tgctgctctt ttctctttcg cgaacatgac
     4921 gctatcaatc cctttcagcg tgacaccacg cagaataaac ggagccatat tgcccatcaa
     4981 atccatcccc tgagccatgc cgcaggctgc aacaatactg ccatatcgga ccccggcgca
     5041 ggcatttctc cagtaacagg gggttgtatt gtattttatt gtgccggttc aggtgtgcgt
     5101 ttcccggcgc gtctgcaccg gcttaaccaa attcaacagg gataaaatag tgcaaataac
     5161 cgaagtatta cttgcagaat cgggggaagc atccggggtt ttattcaaca ggctgtagat
     5221 cactgactgc gtctgttggc cgtccacttc acgcgtcatt cgaccagagt aaatattgat
     5281 gattactaga ttcgtgtatt ttatcaggca ttttacttgc gaataaatcc ccttgctatc
     5341 cagcgaaacc acacaggtag ctattaccat atgtggtaat acactagtta caggaaaaat
     5401 atgcaagttc cgcaatacgt tgtgtgttgc tccttagcca ggatcttttc ttccatgcga
     5461 gatatgacaa caattttgag gaaggatgtt cgtaactggt agagttacta caacaagcat
     5521 gggaggctct gactctgaca gacattgtac tgtggaatgt tgttacaggt tggggcggca
     5581 accgtgccag gagaccatga gccgttcacg gcattaaagg ccacacttca gtcgtttaca
     5641 ttccctatgg tggcaggcat cttccattaa ggcgtcagag cgttaccaac gttatgttgt
     5701 catggctcat ctttcatagt cgatgcttat ctggtggctg aagctagccg cagataaaga
     5761 gtgtgaagtt gataccatgg agtggagtga cgtgcaggca ggagattcgc tctatgcagg
     5821 gttgtgagat gaattcaaac cggctggcag cgccagtgat attcgaagat tcgtccggtt
     5881 gttacccagt atgcattaaa aacccagaca ttatggacaa tataacggct tctacaacaa
     5941 ggaagtgcct gtttaccatc cgggtgtaat gactcacctt tgtgggcttt tgcccgtcgc
     6001 gctcttaacg ttgaagacat tctctgcctg ccgattcagg actgcgacaa aagccatatc
     6061 tggctactgg tcaaggatga ccagcgtctg gagtaggtga taagccaaat agataagctg
     6121 gaatatgtgg tgaaagtgca acgtaatcag tccaacccga cgatgtttaa caagattgtg
     6181 gtgttttttc agtaagtcat tcgtcttctg aatcaggtgt aatgatcttt acagaaccat
     6241 tctggcagtg cgtaacataa tctgccggta acggacgatc gctaatcaga acgtcaaatg
     6301 cagagatcgg tgcgaggctg gcagaggcca cttcatcgaa caacgcgtgg cgggcgagca
     6361 aaattttccg tagtccacgg ttcatcgctt tacgcttagt ggcaagatct tcagggttaa
     6421 accagctgac gccaaagtga ttatgcacac cgctggcgga aataaatatt tttcgcggat
     6481 tgagagagtc taatggcgaa gagttactgg catcgtaaaa agcatcactt ctggcacgat
     6541 acgtaccacc acaaagtatt gctgttacat taggcttttc attcaacgca acaaagacgc
     6601 gatgtgagta acagatgccg gtgaaggtga ttgcatccgg gatcatgctt ataacgagtg
     6661 gtatctcctg gccattatca aagaagatca gatcattttc attaaccatt ccggcagcca
     6721 gaattgcaat aggtaagtca tcacgatgat tttttggaac gtcatggatt actggtatgg
     6781 atggcgcggg tttattcacc attacaatat agccacccag tagggtcagt ggcagaggtt
     6841 catcttcctg atggagatcg cgacgaatag tcattacaga aacttccagc attcgcgctg
     6901 cgtctttcaa atggattctg tcggttttct taagcagttc catcaaacgt cggatacgct
     6961 ctttttgctt cgtttccatc aactctctct ccgtaagaat gttataaaaa taacattttg
     7021 gcgttagtaa acattgtttt cattaccata ctatacgacc gcccctttga aatgaaaagg
     7081 gtataggctg ctgagttttg ggcactaatc ttgttaggtt gcttatgatt agatagtgtt
     7141 ggcctgagtg atgagactgg aagtctatta aataaacatc gggttgatgt gctaatcgcg
     7201 atctcagtca caaatgatgc aaaagcaaca tgctaatgtt atttaaatac cattgttatg
     7261 cgaaagttta taagaggtag tgaaatggac attgcagtta ttggttccaa tatggtggat
     7321 ctcattactt acactaatca gatgcctaaa gaaggagaaa ctctggaagc acctgccttc
     7381 aaaatcggat gtggtgggaa aggtgctaat caagcggtgg cggcggctaa attgaattca
     7441 aaagtcctga tgctgactaa agtaggtgat gatatttttg cggataatac tattcgtaat
     7501 cttgaatcat ggggaattaa taccacttat gttgagaagg tcccctgtac cagcagtggt
     7561 gttgcaccga tctttgttaa tgccaactcg agcaatagta tattaataat taagggagcg
     7621 aataaatttc tttccccgga agatatcgat cgagctgcag aagatctgaa aaaatgcaaa
     7681 ttgatcgttt tacaactgga agttcaactg gaaacagttt accacgcgat tgagtttggt
     7741 aagaaaaatg gtattgaagt gttattaaat ccggctccgg cattgcgtga gttagatatg
     7801 tcttatgcct gcaaatgtga tttctttatc ccgaatgaaa cagaactgga aatattaacc
     7861 ggtatgtcgg ttgatactta tgatcatatt cgtttggccg cacgttcgtt agttgataaa
     7921 gggctgaaca atattattgt taccatgagc gaaaaaggcg cattatggat gacccgcgat
     7981 caggaagtcc atgttcctgc attcaaagta aatgctgttg atactagtgg cgctggtgac
     8041 gcatttattg gttgcttttc ccattattat gttcaaagcg gtgatgttga agccgctttg
     8101 aaaaaagccg ctctctttgc cgctttcagc gtcacaggga aaggtacgca atcatcttat
     8161 ccgagtattg aacaatttaa tgaattttta accttaaacg aataatcatc ctgcacagtt
     8221 aaaggtagca ctatgaacga taaaaacatc attcagatgc ctgatgggta tctgaataag
     8281 actcccttgt tccagtttat tttgttatcg tgtttattcc cgctatgggg atgcgcagct
     8341 gcattaaatg acatattaat cactcaattc aaaagtgtat tttcgctaag taactttgct
     8401 tcagcattgg tgcagagcgc attttatgga ggttattttt taattgcaat accagcatcc
     8461 cttgtgatta aaaagaccag ttataaggtg gcaattttaa ttggcctgac gctgtatatt
     8521 ggtggttgta cacttttttt ccctgcctcg catatggcaa cctacactat gttccttgca
     8581 gctatttttg cgattgcgat tggcctgagt ttccttgaaa ctgcagcgaa cacatacagt
     8641 tcaatgatcg gtccaaaagc ctatgccaca ttgcggctca atatcagcca gaccttttat
     8701 ccgattggtg ctgcgtctgg tattttattg ggcaaatatc tggttttctc cgagggggaa
     8761 agccttgaga agcagatgtc cggaatgaac gccgagcaaa ttcacaactt taaggtgctg
     8821 atgttagaga acacccttga accgtataag tatatgatta tgattctggt tgtggtcatg
     8881 gtgttgttcc ttttaacccg cttcccaacc tgtaaagtag cacagacaag tcaccataaa
     8941 cgcccgtcag caatggatac gctgcgctat ctggctaaaa attcgcgctt tcgtcgtggt
     9001 attgtggctc aattccttta tgtgggtatg caggtcgcag tatggtcatt taccatccgt
     9061 ctggcactgg agttgggcga tatcaacgaa cgtgatgctt caaacttcat ggtctacagc
     9121 tttgcctgct tctttattgg caagtttata gccaacatcc ttatgacacg ttttaatccg
     9181 gaaaaagtcc taattctcta ttccgttata ggggcgctgt ttctggcata tgttgcactg
     9241 gctccaagtt tcagcgcggt ctatgtcgcc gtcctggtaa gtgttttgtt tggcccttgc
     9301 tgggcgacga tctatgcagg aacactggat acagttgata accaacatac agaaatggca
     9361 ggagcagtca ttgttatggc tattgttggc gcagcggtgg ttccggcaat tcaggggtat
     9421 attgcagata tgttccactc actacagctt tcattcctgg tttctatgct ctgttttgtc
     9481 tatgtcggtg tctacttctg gcgtgaaagc aaagtacgta atgcactggc tgaagtcaca
     9541 gagtcctgag gaaaatcgtt atgagtacgc gtatttattt gtggcgggcg ctttttggcg
     9601 aaaaacctcg tatcttgctg gaaaatagtg attttactgt cacgtctttt cgctatgaca
     9661 gtggtgtcga ggggcttaaa attgcaaaca gtcgtggaca tttaattatt cttccctgga
     9721 tggggcagat gatttgggat gcgcaatttg atggacacag tctcaccatg tgcaatatgt
     9781 ttcgccagcc taaaccggcg acagaggtga tagagaccta tggctgtttt gcctttcatt
     9841 caggtttgct ggctaatggc tgcccatctg cggaagatac tcatctgcta cacggtgaaa
     9901 tggcctgtgc cgcgatggat gaagcatgga tggaattaga gggagatatg ttacgcctta
     9961 ccgggcgtta cgagtatgta atggggttcg ggcatcatta tctggctcag ccaacagtgg
    10021 tccttcacaa atcctctaca ttgttcgata ttaagatggc cgtaaccaat ctggcttctg
    10081 tagatatgcc actacaatat atgtgccaca tgaattatgc atatatacca aatgctacgt
    10141 tcagccagaa tataccggac gaaatattaa ggttgcgtga atcagtacct tcgcatgtta
    10201 atcccaccgc tcagtggctg gcgtttaatc aacggattat gcagggagaa gcctctctgt
    10261 cgacgctgaa tcaaccggaa ttttatgatc cagaaatagt cttctttgca gacaaactgg
    10321 acgcttacac agatcagcca gagtttcgca tgatcgctcc cgatggcact acgtttgtta
    10381 ctcgtttctc cagtgccgaa ctaaattatg tgacacgctg gatcctttat aacggcgagc
    10441 aacaggtagc cgcctttgca ttacctgcaa cctgtcggcc tgaggggtat cttgccgcgc
    10501 aacgtaatgg cacgctgatt caggttgcac cgcagcaaac acgcactttt acagtcacca
    10561 caggaattga ataaccactt aaggcctgaa cagcggcgcg cttatcgtta aggtaagcgc
    10621 gtttttttac ccgccaggac aagactatga tagcgttgcc cttatagacg atcacattat
    10681 cgtccactcc aaatttgcgc aactactgta gagtggtgta ctccacagct tcttttagga
    10741 tctcaacttc cattgttttc ttaccgagca agcactgcaa ctcttttatt ttgaagtggt
    10801 caacaaaaac tggccaccga gttagagttt ttccagtatc gattttccga ttcgtttggg
    10861 ggtaacccac cgttatattc gtgcggtctt agtgcgctgt aatatccaac gatatagtcc
    10921 gttatggcgt gagctgcctc gctgaagctt acgtaaccca ccaccggcat ccattcgttc
    10981 ttcagactcc tgaagaagcg ttccattggg ctgttatccc agcagtttcc gcgccggctc
    11041 atactctgtc tgatctggta tcgccacaat aactgccgga actgcctgct cgtataatga
    11101 ctgccttgat cgcttggaac atcaccccga cgggcttacc acgggtttcc catgccattt
    11161 ccagtgcttt catggtaagc ctgctgtccg gcgagaacga catggcccag cccactggtt
    11221 ttcttgcgaa caggtcgaga acaacggcga ggtacgccca gcgcttaccc atccagatat
    11281 aggtcacatc accgcaccac acctgatttg gttccgttac ggcgaactgt cgctcaagat
    11341 gattcgggat agcaacgtgc tcatgaccgc cacgcttata ccggtgagtc ggctgctggc
    11401 aactgaccag ccccagctct ttcatgagtc tgccagcaag ccagcgcccc atctggtagc
    11461 ct
//
LOCUS       DBBGLQ010000128        11393 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.128, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000128 DBBGLQ010000000
VERSION     DBBGLQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.128"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..271
                     /locus_tag="ACIYSB_004551"
     CDS             <1..271
                     /locus_tag="ACIYSB_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGC5431454.1"
                     /translation="ECYFTQKLASYLITHSGNYRYNSTESALLTHREKEILNKLRIGA
                     SNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            276..665
                     /gene="csgE"
                     /locus_tag="ACIYSB_004552"
     CDS             276..665
                     /gene="csgE"
                     /locus_tag="ACIYSB_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGC5431455.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            690..1106
                     /gene="csgF"
                     /locus_tag="ACIYSB_004553"
     CDS             690..1106
                     /gene="csgF"
                     /locus_tag="ACIYSB_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGC5431456.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1133..1966
                     /gene="csgG"
                     /locus_tag="ACIYSB_004554"
     CDS             1133..1966
                     /gene="csgG"
                     /locus_tag="ACIYSB_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGC5431457.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2030..2521)
                     /gene="ycdZ"
                     /locus_tag="ACIYSB_004555"
     CDS             complement(2030..2521)
                     /gene="ycdZ"
                     /locus_tag="ACIYSB_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGC5431458.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2623..3177)
                     /gene="ycdY"
                     /locus_tag="ACIYSB_004556"
     CDS             complement(2623..3177)
                     /gene="ycdY"
                     /locus_tag="ACIYSB_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGC5431459.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3201..3938)
                     /gene="ycdX"
                     /locus_tag="ACIYSB_004557"
     CDS             complement(3201..3938)
                     /gene="ycdX"
                     /locus_tag="ACIYSB_004557"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGC5431460.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCREVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3993..4931)
                     /gene="ghrA"
                     /locus_tag="ACIYSB_004558"
     CDS             complement(3993..4931)
                     /gene="ghrA"
                     /locus_tag="ACIYSB_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGC5431461.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5165..5252
                     /locus_tag="ACIYSB_004559"
     tRNA            5165..5252
                     /locus_tag="ACIYSB_004559"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5199..5201,aa:Ser,seq:gga)
     gene            complement(5402..6244)
                     /locus_tag="ACIYSB_004560"
     CDS             complement(5402..6244)
                     /locus_tag="ACIYSB_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGC5431462.1"
                     /translation="MSDITISRPELVTEHTDVICSTSIHHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTAPDWAMKQDFCCGCWLMEKPETAMKAITRNLDREIWRDLMRRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFKQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGNKIIVNNLVRWDRWGFHLNSGQQADRLADLERMLYLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6341..6538)
                     /locus_tag="ACIYSB_004561"
     CDS             complement(6341..6538)
                     /locus_tag="ACIYSB_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGC5431463.1"
                     /translation="MKSLATETALDILIAWLQDNIDCESEIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQLQFLHQNR"
     gene            complement(6550..7038)
                     /locus_tag="ACIYSB_004562"
     CDS             complement(6550..7038)
                     /locus_tag="ACIYSB_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGC5431464.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRTSAERTPPSAALTGIRCSTAHITETDNAWLFSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIQRYGVSLIHLDASAECLPDLPTF
                     GW"
     gene            complement(7035..7412)
                     /gene="cbtA"
                     /locus_tag="ACIYSB_004563"
     CDS             complement(7035..7412)
                     /gene="cbtA"
                     /locus_tag="ACIYSB_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HGC5431465.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPGAKQ"
     gene            complement(7501..7869)
                     /gene="cbeA"
                     /locus_tag="ACIYSB_004564"
     CDS             complement(7501..7869)
                     /gene="cbeA"
                     /locus_tag="ACIYSB_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGC5431466.1"
                     /translation="MSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCKAD
                     TLSSCGYVYLAVYPTPKMKN"
     gene            complement(7919..8563)
                     /locus_tag="ACIYSB_004565"
     CDS             complement(7919..8563)
                     /locus_tag="ACIYSB_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HGC5431467.1"
                     /translation="MSRIITTTVYTLDELSCPAQEKARDGYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPSIWFSGFCSQGDGACFEGRWHWQPATVRRIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQTMT
                     TDAEAAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            complement(8578..8799)
                     /locus_tag="ACIYSB_004566"
     CDS             complement(8578..8799)
                     /locus_tag="ACIYSB_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGC5431468.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8868..9344)
                     /locus_tag="ACIYSB_004567"
     CDS             complement(8868..9344)
                     /locus_tag="ACIYSB_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGC5431469.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(9359..9844)
                     /locus_tag="ACIYSB_004568"
     CDS             complement(9359..9844)
                     /locus_tag="ACIYSB_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGC5431470.1"
                     /translation="MTTVLHNSTTPSVSVTAVSENNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFSA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECNAIMRII
                     D"
     gene            complement(9935..10753)
                     /locus_tag="ACIYSB_004569"
     CDS             complement(9935..10753)
                     /locus_tag="ACIYSB_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGC5431471.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEYQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLKSMR"
     gene            complement(11080..>11393)
                     /locus_tag="ACIYSB_004570"
     CDS             complement(11080..>11393)
                     /locus_tag="ACIYSB_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5431472.1"
                     /translation="SSRDTLRDSAKHRVSELPVNWWVQPSVIRTFSSRGDMSMGTAEA
                     GSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGNSAEGYNGQATLNVT
                     F"
BASE COUNT         2783 a         2923 c         2908 g         2779 t
ORIGIN      
        1 cgaatgctac tttacgcaaa agcttgccag ctacctgatt acgcattcag gtaactatcg
       61 ttataacagc acggaatcag ccctccttac tcatcgggaa aaagagatcc tgaataagct
      121 gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg aaaatacggt
      181 taaaacgcat ctttataatc ttttcaagaa gatagccgta aaaaaccgga cacaagcggt
      241 ttcctgggca aacgataacc tcaggcgata aagccatgaa acgttattta cgctggattg
      301 tggcggcaga atttctgttc gccgcaggga atcttcacgc cgttgaggta gaagtcccgg
      361 gattgctaac tgaccatact gtttcatcta ttggccatga tttttaccga gcctttagtg
      421 ataaatggga aagtgactat acgggtaact taacgattaa tgaaaggccc agtgcacgat
      481 ggggaagctg gatcactata acggtcaatc aggacgttat tttccagact tttttatttc
      541 cgttgaaaag agactttgag aaaactgtcg tctttgcact gattcaaact gaagaagcac
      601 taaatcgtcg ccagataaat caggcgttat taagtacggg cgatttggcg catgatgaat
      661 tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta gttctactca
      721 tgcttatttc gccattaagt tgggctggaa ccatgacttt ccagttccgt aatccaaact
      781 ttggtggtaa cccaaataat ggcgcttttt tattaaatag cgctcaggcc caaaactctt
      841 ataaagatcc gagctataac gatgactttg gtattgaaac accctcagcg ttagataact
      901 ttactcaggc catccagtca caaattttag gtgggctact gtcgaatatt aataccggta
      961 aaccgggccg catggtgacc aacgattata ttgtcgatat tgccaaccgc gatggtcaat
     1021 tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc gaccatccag gtttcgggtt
     1081 tacaaaataa ctcaaccgat ttttaagccc cagcttcata aggaaaataa tcatgcagcg
     1141 cttatttctt ttggttgccg tcatgttact gagcggatgc ttaaccgccc cgcctaaaga
     1201 agccgccaga ccgacattaa tgcctcgtgc tcagagctac aaagatttga cccatctgcc
     1261 agcgccgacg ggtaaaatct ttgtttcggt atacaacatt caggacgaaa ccgggcaatt
     1321 taaaccctac ccggcaagta acttctccac tgctgttccg caaagcgcca cggcaatgct
     1381 ggtcacggca ctgaaagatt ctcgctggtt tataccgctg gagcgccagg gcttacaaaa
     1441 cctgcttaac gagcgcaaga ttattcgtgc ggcacaagaa aacggcacgg ttgccattaa
     1501 taaccgaatc ccgctgcaat ctttaacggc ggcaaatatc atggttgaag gttcgattat
     1561 cggttatgaa agcaacgtca aatctggcgg ggttggggca agatattttg gcatcggtgc
     1621 cgacacgcaa taccagctcg atcagattgc cgtgaacctg cgcgtcgtca atgtgagtac
     1681 cggcgagatc ctttcttcgg tgaacaccag taagacgata ctttcctatg aagttcaggc
     1741 cggggttttc cgctttattg actaccagcg cttgcttgaa ggggaagtgg gttacacctc
     1801 gaacgaacct gttatgctgt gcctgatgtc ggctatcgaa acaggggtca ttttcctgat
     1861 taatgatggt atcgaccgtg gtctgtggga tttgcaaaat aaagcagaac ggcagaatga
     1921 cattctggtg aaataccgcc atatgtcggt tccaccggaa tcctgacaga taaaaaagcc
     1981 ggaattatca tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2041 ttttgatttc ctgctcacgg tgcgccgtct ttgcactacg cgccgccagc cacaggccac
     2101 tgtttttcat tgcgtaacca aatatcagcc caagcgccag cgaaggtaac accagtttcc
     2161 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2221 gcagcagctg tttggcctga atacacatca gaaacgcgac aataccggtt atgacataac
     2281 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2341 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2401 caaagtaggc cgtacagccc aggaagcccg cccagcttag taagcccagg gaaacagcca
     2461 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2521 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2581 caacaaatta tccgttgcag ttatgagtga cgtagatcac acttattctt cagaatcttc
     2641 ttccagctcg tcccacattg cactaatggc atcgcgggtt agcggtgcca tggtgcgcca
     2701 gaaaggcgtg gttgcatggg cctccacttt gccaaggaac gcaccacacc agggtaacag
     2761 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     2821 ccaggaagct gcgagcagca atgtgccgat gtgatcggct ggcgtatccg ctaatggcat
     2881 ccctcgctca gaaagaaaag cgcgcacttc cgcttccgtc gcgccctcaa cccatgcgct
     2941 acgatatggc ggcacagcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3001 ttgcgtcata tcacagcttt tctgcaaacg tgtcagcaac tcatcctgct ccagtggcca
     3061 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3121 tggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3181 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3241 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3301 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3361 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc tacctcacga
     3421 cagttgtctt cactgccctt acgtgagtgt aaaaatgagg aattattgat ttccagcgcc
     3481 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3541 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3601 ttggtcgctt tgtcatgtgg cgcaaaaacc ggctcatgaa aaccggcaat aattagatcc
     3661 agcgagtcaa acattttacc gctgcagtca atttcaccat caacattttt aatgttagct
     3721 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     3781 aagtgccagt gatgcggcgc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     3841 ttaatgccct tttgtttggc ctgggcaatg taatcactta atgtgctata tgcatgtgtg
     3901 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     3961 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4021 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4081 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4141 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4201 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4261 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4321 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4381 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4441 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4501 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4561 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4621 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4681 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4741 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     4801 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     4861 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     4921 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     4981 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5041 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5101 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5161 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5221 tatcgggggt tcgaatcccc ccctcaccgc cagattacaa gcgcatgtta ttgtttaaca
     5281 tgcgcttttt tatttgtcct tccggacagt atgccatcgg gtatgccatc tgaaaccggt
     5341 ttatatgccc cacgatcata tcttatcgga caatttccga tctttgagca tactggcaaa
     5401 gctatactgc cagcatgtca ggatagtgtt ttgcgataat atcattcagt ctgtcaacca
     5461 gttctggttt tctgaacgtg atatgcgcgg agcccttctt aaagtatctg atgctgaaca
     5521 tctcatcttc atagtcctct ttaccctgaa cagactggat gtggtcatcc agatgaatgg
     5581 tgatgttttc ccggttgtcg gggatcggtt tgccgctgaa cagatacagc attctttcca
     5641 ggtcggcaag tcgatctgcc tgttgcccgc tgttcagatg aaatccccac cggtcccatc
     5701 tcaccagatt gttgacgata attttattac caaatttaca gggggagttg gttttgtaat
     5761 tccagctcaa ccctctgaag acgttgatca ccccccgctc aaacacttca tccttattct
     5821 ggtgcagttg tttaaatgtg ctcagaatgt tcgcttcact gatttccgga aaattatcgt
     5881 attccagtga ccggtaccat gtatcacggg cctgtgcatc cattaaggaa agcatcccgg
     5941 aacgtcgcat caggtcacgc cagatttcgc ggtcaaggtt gcgggtgatg gctttcattg
     6001 cggtttccgg tttctccatc agccagcagc cgcagcaaaa atcctgtttc atggcccagt
     6061 ccggggcggt tttaccgcca atactttctg tcattgctga tatttcagcc agttgccgga
     6121 taagtgtgtc gatttgcagc agtgtactct ttcgtacagc cagaatgtgg tggatactgg
     6181 ttgagcagat aacgtccgta tgctcggtga ccagttccgg gcgggagatt gtgatgtctg
     6241 acattatcgg tttccctttt ggtttataaa cgaaaacgcc agccctgttc aggctgacgt
     6301 cagtggaatg aagtctggtg agtgagatga tgagtaagac tcaccggttc tggtgcagaa
     6361 actgcaactg gcgcagggta tggatatcct ccctggcctg ttcaatgcag ggcagcagtg
     6421 ctgccgaatc cgttttatcc tcatcattgt caaagataat ttccgattcg cagtcgatat
     6481 tgtcctgcag ccacgcaatc agaatatcca gcgccgtttc tgtggctaat gatttcatgt
     6541 tgttcctggt taccagccga aagtgggcag gtccggcaga cattcagcgc tggcatccag
     6601 atgaatgaga ctgacgccat aacgttggat gagtgtgaca accagacgac ggaacgtttt
     6661 tgacagcccc aggcgtttaa gccgcagaac cgggtatgac cacgcatcgg tacgtaacag
     6721 atatccgcta cctgtgaaat gaatccattc tgattcgccg gtgtcattcg tctgatgtga
     6781 cagcgagaac aaccaggcat tgtccgtttc cgtgatatgt gcggtactgc agcgtatgcc
     6841 ggtaagggcc gcagatggtg gcgtgcgctc tgctgatgtc cggtcagatt catcaacaac
     6901 acgaagggag taaccgttat cgcagaccac gttaatcagt tcagcaagag taacaccatc
     6961 gacgtcaacg acaatgcgcc ccatgttcag tgcctgcacg ttaatgctgt cggcttccag
     7021 cgtcagggcc agtttcattg tttcgccccc ggatgtttac ccagggtaat gttatttacc
     7081 gttctgtaat tgtcgcgggt catcaggcct gtcgcccggc gggcccggag gatatcgatg
     7141 ctgtttatta actgagagtg ggtacaggcg ctgaatcccg gctggtcggt acgcaccagt
     7201 gcgtattttt ccacgagaaa gttcaccgca tcacacagtg aaatgccagc ttcaatatgc
     7261 tgctcaatca cacgttcatc ggcaaacggt gtgtcattca gtgtgaggcc gtagtgctgg
     7321 tccagcagtc gggacagcag tatctgccag atttcaacag gagacgggcg agaactggcc
     7381 gcctgcccgg gtaatacagg taatgttttc atactgaaga ttttcctgat atgcagatat
     7441 aaaaatggga aagtggcgtg gtgaaaacac caggccgtag cagaaggcta ttctggagag
     7501 ttaatttttc attttgggcg tcggataaac agccagataa acgtaaccac aactgctgag
     7561 ggtatcggct ttgcaggtca gccctctggc atacagcgtg acggtatgct gatggcgggg
     7621 attcagttca ccgctggtga gcatgagctc cagttgtttc atcagcagcg gaaaggcctg
     7681 gtccagatgg tacgcatccg cgtcgctgaa ccggcctctg ataccggcgc ggtcggcaag
     7741 gtaatgcaac tgattgccct cctgcaccag acgggcgccg aaacagggcg tcacggtgca
     7801 gggtagtccc caccagggac ggtcatgatt gtcatcggga tgcgttgtcc cggagagtgt
     7861 gtctgacacg ataaaatcct cacagaaaat cggtgaatga tagtttaacg atgacgagtc
     7921 agccggcgcg cagaccggct tcggtgaagg tgtattcgtt gatgagtagg gcctcatcca
     7981 cagcggcgtc tgaggtcagc cagtcgtact cgttttccag ttgacggtac aaccagaagg
     8041 ccaggtcgcg cagggcttca ctgactgcgg cttccgcatc ggttgtcata gtctgaccag
     8101 tcggactgtt cctggtaaca gtgatatcca tgctgtacgg atggcagtaa cgtccccggt
     8161 gactgatttc agcctgcagt tgccagaagt tacgcttctg caccgcctgc agggcatccg
     8221 cgatacgatg aagctcacgg tcctgcgggg catactcccg gattcttctg actgtcgctg
     8281 gctgccagtg ccagcgtcct tcaaaacagg ctccgtcccc ctggctgcag aatccgctga
     8341 accagatgct cggctcttcc ataaacctgc cgttgctgag ccggaaaacg cgctggcgga
     8401 ggtcaattcc gaaaatgcca cagacctccc tgaagtcttc atacacgttt tcataccagt
     8461 tgctgtcagc atgatgctga cggtacccgt cgcgggcttt ttcctgtgcc gggcaggaaa
     8521 gctcatccag cgtatacacg gtggtggtga taattctgct catcagaatg ccctgattca
     8581 gttcagtgtg acgctcatca gccggacata cgggccaaaa ctgtccttac ggcgttctgc
     8641 aaacacagcc agcacaccgg gaatatcctg cacttcacgg ccggtatatg tatccgtgct
     8701 accgtgccag cggtatttgc cggtacagaa cggaaaaaga cgggatgccg gatgttgttg
     8761 gtgaatacgc atggcttcac cacgggtgat gattttcatg gtgtgatacc tcttaagaca
     8821 gaagataaaa gtgaaaacag gtgtgatgtg gtggtgacgg tgacgggtta aagcagaccg
     8881 tgttccgcaa aggagaaaac ctggctgcca ccgactatca gatggtccgg tacccggata
     8941 tccaccaggc ccagtgcctg taccagacgt tccgtgataa ggcggtctgc cttgctgggt
     9001 gtgacttcac cggacgggtg attgtgtgcc agcaccacgg cagcggcatt gtggtacagg
     9061 gcacgtttaa tcacttcccg gggatggact tccgtgcggt tgatggtgcc ggtgaagagg
     9121 gtttcaccgg caatcagctg attctggttg ttcagataca gcacccggaa ctcttcccgc
     9181 tccagtcccg ccatgttcag aatcagccac tcccgtgccg catgcgtgga ggtgaaggcc
     9241 acgccgggtt catgaagatg gcggtccagg gttttcaggg cccgctgaat gaggctgcgc
     9301 tcgccgggcg tcatctctcc gggcagaaag gaaagctgtt gcattgttcc ggcccctttc
     9361 agtcaatgat gcgcataatg gcgttgcatt ccggatgctg cagggcgtaa tcccgcagcc
     9421 ggtaataatg gaccgtcatg gcataacact ccgtacgaca ggcatgatgg ctgtacgtca
     9481 tcagacaggc agcaatgccg gcggcttccg ggctcatttc agcgcggtta ccgttcatgg
     9541 cactgaacag tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc
     9601 cgccgttgtt cagggtgtac agattccaga taccaccaca gtagtcttcg cacagacgat
     9661 ccatccagcc gaagacacgg ggctccaggg tcacccactg tggaatgagg ccaaaatgct
     9721 gcggccagaa gctgatgcgc tgttcatcag ggacgagggt ggcaaccagt tgaggcgggt
     9781 tattctctga tacagcggtt acggaaacag aaggtgtggt ggaattatgc aaaacggttg
     9841 tcatgagatt attccttata aaaagtaaat gaatggaaga agtcgcgggg cagggacgga
     9901 catgagtcag aactgcgctt tcagggaaaa ggcatcagcg catactcttc agcagtgttt
     9961 ctgccatcac ccacaatgcg cgattgagct taatgtctgt gtcgatgctg tgaatggcac
    10021 gggtatggat acgttttcct ctggcactgc gaccggaaat cccgcctttc agcatattct
    10081 cctggatggt ctgataagca ctccacaggt ccttaccgta atcctcccgg cgtcgcggtg
    10141 tcagaatgtc agcagtggtg acgggctgat attcatcacc ataacggtaa gtcagtgccg
    10201 cctgtgccag cgcctggcgt gccggtggcg gcaggaccag cgactgcatg gcatcacgct
    10261 tctcctcaat ccggtcaaaa acccccacca cctcgtaagc cccttcgata actttctcca
    10321 ctacgtctcc ccggtgtgga acacgcactt cccccagaga ctgaccacag acgcatccgt
    10381 tctggcagac gaacctgaag taacccggca gcatctggta gctggaggta ccgtcatggg
    10441 agttcagcag gatgatttca gggacatgtt gtccgtttat ctccccgtca cggcgcagac
    10501 gcagcatgtg tttggtgtat ccccggcggc ccgggtcgcg cacgcgggtc tggcaggcga
    10561 agaatggctg gaagccttcc cgctgcaggc tttccagcac ggtgatggtg gggatccagg
    10621 tatagttctc gcttctggat gtgtgtttct cttccccgaa tacgctgggg acgacctgca
    10681 tcagttcttc acgggttaag gggcggtcac ggcgtatctg gttcgcataa ccaaaacgac
    10741 tggctaatcg catgatttat tccttatgaa tgagttagcg gtataaatga aaaagccgcg
    10801 tctcccggag aagacgcggc ctgatggacg aaatgaatga cgtttattgt ctgagaagcc
    10861 cttcactggc gagctgcgta tttttatgga aggagatcac ccaataaccc catcgggatc
    10921 gggcaataac cggccaaatt gtgaacaatt cagatgccat ttggcagcaa aagagccttc
    10981 ggcaataaac gtgatgtaca acccggcata tctgttgctc cctgaaagta aaaagccccg
    11041 gtcattatga ccggggcctg aaggggagtg acctgattat cagaaagtca cattcagcgt
    11101 ggcctgaccg ttatagcctt cagcgctgtt gccgctgaca ctgtgggcat aaccggcctg
    11161 aacgcccaga gtgatatttt cccggacacg ggcttccagt ccggcctgca ggtccagtga
    11221 cgtgccattc tgtgacggtg agaacgtcat gttactgccg gcttcagctg tacccatgct
    11281 catgtcaccc cgggagctga aagtgcggat aacagaaggc tgtacccacc agttcaccgg
    11341 cagttcactc acacggtgtt ttgcactgtc gcgcagggtg tcacgggatg agg
//
LOCUS       DBBGLQ010000129        11264 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.129, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000129 DBBGLQ010000000
VERSION     DBBGLQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.129"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..149)
                     /locus_tag="ACIYSB_004571"
     CDS             complement(<1..149)
                     /locus_tag="ACIYSB_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HGC5431473.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     DYFRMA"
     gene            complement(154..852)
                     /locus_tag="ACIYSB_004572"
     CDS             complement(154..852)
                     /locus_tag="ACIYSB_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGC5431474.1"
                     /translation="MQDLQPQTLNECTKSQQSALVVLWEIDLTEVGGERYFFCNEQNE
                     KGEAVTWQERQYQPYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSIHKLSQ"
     gene            complement(852..1193)
                     /locus_tag="ACIYSB_004573"
     CDS             complement(852..1193)
                     /locus_tag="ACIYSB_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5431475.1"
                     /translation="MGELKIFRWLPREGMETTRKPSVITVKFGDGYEQRRAGGLNADL
                     KTFKPVFRVTDEYSRAALDSFLSRHAGVRAFLWRPPKYNRTVRVVCREWSISDNAMYT
                     DFSCTFEEVTH"
     gene            complement(1186..4425)
                     /locus_tag="ACIYSB_004574"
     CDS             complement(1186..4425)
                     /locus_tag="ACIYSB_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5431476.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AVSHEMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISLADEWNSVNARLKQAS
                     QSSDDFQSSQRELMAISQRTGTAFSDNASLFARSAASMREYGYSSEEVLKVTEAISTG
                     LKLSGASTAEASSVITQFSQALAQGVLRGEEFNSVNESGDRVIRALAAGMGVARKDLK
                     AMADNGKLTADKVVPALISQLGALRDEYAAMPDTVSSSATKVENAFMAWVGGANEASG
                     VTKTLSGVLNGIAGNIDTVATAAGALVAVGVARYFGNMASSAGSATAGLITAARNEVA
                     LAEVQLRGTQIATARARAAVYRAQQAVVAARGTERQAAAEAKLTAAQASLTRNIAART
                     AAQTTLNNVTSVGSRLLSGALGLVGGVPGLVMLGAAAWYTMYQNQEQARESARQYAAT
                     IDEIRQKTSAMSLPEASDNEEKTRQALKEQNRLIDEQKSKIKSLQEKIAGYQYVLANP
                     GWTTDNGFMINHMTSVKTVTEGLAEATNQLAVEQSRLTQMQGKAQSIQDVLAGLEERR
                     VALIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQTALNNSERELALSRLKGEARERARLGYAADDLGFVGEAYQTARLNYINNSLDAW
                     RNNQANKPKAHKKTEAEKTEDTYKRLIKQQKEQIALAGQNTELAKMKYQVSKGELSTL
                     SEAQKKTLLQNAALIDQKKIREQLAAYESSLADSNASVRASNEAQLLGYGEGSRMRER
                     LQEMWSIRQEFEQKNNELLRQYQAGEIEEALWKQEKSLNEKYLEERLSDQQDYYAKAD
                     ALRSNWNAGLQEGLTNWADSATDYASQAADAVVSTMDGLVSNISDALAGNVVDWRNWG
                     SSILQEVSKILMNAAIVNGLKSLSKSMSGAGGWLGTVGDWLSGAVANAKGGVYTSANL
                     SAYSNTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVNGGGGFVY
                     SPVYHISIQNQGSNGEIDARSARGLVDLIDSRVVSVMQSSRRDGGLLSG"
     gene            complement(4472..4762)
                     /locus_tag="ACIYSB_004575"
     CDS             complement(4472..4762)
                     /locus_tag="ACIYSB_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGC5431477.1"
                     /translation="MRFLMMLALRMGRTLSELRREMSASEIMMWAEFDRFSPLGDERA
                     DIRAAQIVSAVYGAQGVKVPLNDALLQWEQELTEGASDPFAGLENALLTVSQ"
     gene            complement(4774..5145)
                     /locus_tag="ACIYSB_004576"
     CDS             complement(4774..5145)
                     /locus_tag="ACIYSB_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGC5431478.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLKADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            complement(5160..5864)
                     /locus_tag="ACIYSB_004577"
     CDS             complement(5160..5864)
                     /locus_tag="ACIYSB_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HGC5431479.1"
                     /translation="MPSNFERSQLTKIMISSVPVRKETLDSASFLELSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYVNAAQNALRSAYDNDATYGFKVI
                     FPSGNGFTFMAEVRQHTWSAGTNGVVAATFSLRLKGKPVLTTQLLKVKLDLKSTLKVS
                     TGNKLEMVVEAAGGVPPYSYVWKKGGSPVAGQTAATFSKASAVSGDAGAYTCEISDSA
                     SPVNKVTSTSCTVTVS"
     gene            complement(5924..6268)
                     /locus_tag="ACIYSB_004578"
     CDS             complement(5924..6268)
                     /locus_tag="ACIYSB_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HGC5431480.1"
                     /translation="MREAALYSRLSELAGGQVYPYVVPLTEGKPAVSPPWLVFSVVSD
                     TASDVLDGQAESRITVQIDVWARIPDDADNIREQALDAVRQLAPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(6265..6714)
                     /locus_tag="ACIYSB_004579"
     CDS             complement(6265..6714)
                     /locus_tag="ACIYSB_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGC5431481.1"
                     /translation="MMDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEIMRDA
                     VVERAPEQTGKLKKNVVVLTQRSKRRGEIISGVHIRGRNLRTGNSDNSMKASDPRNAF
                     YWRFVELGTVNMPAHPFIRPAFDTTEELAARVAIQRMNQAIDEVLSK"
     gene            complement(6711..7049)
                     /locus_tag="ACIYSB_004580"
     CDS             complement(6711..7049)
                     /locus_tag="ACIYSB_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGC5431482.1"
                     /translation="MQAGRLRDRVVILNATTVRSPSGHPVETMTEGATVWAEVKGISG
                     RERISGGAETAQATVRVWMRFRRDVTATSCLKVLTGAFKGAILSIDGPPIPDARATRI
                     EILCSQKGNV"
     gene            complement(7058..7375)
                     /locus_tag="ACIYSB_004581"
     CDS             complement(7058..7375)
                     /locus_tag="ACIYSB_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGC5431483.1"
                     /translation="MAAIVEKLRTQCRIDTDDATDDELLMLYFRAACRKAENFINRKL
                     YEETVPEDDPEGVLIADDVLLALMLLVGHWYENRENSSDVSKAPIPFGFSSLLEPYRF
                     IPL"
     gene            complement(7452..8669)
                     /locus_tag="ACIYSB_004582"
     CDS             complement(7452..8669)
                     /locus_tag="ACIYSB_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGC5431484.1"
                     /translation="MKLHEMRQKRNTIAKDMRALHEKIGDNAWTDEQRAEWNRAKAEL
                     DALDEQIAREEELRRQDQAYVDESGPEERQNNEAENGKKAVEEKRAAAFNRFLRAGFA
                     ELNAEERNLMRELRAQGVTTDSQGGYTVPTQMRNKIIDAMKAYGGIAGVAQLLTTSTG
                     QDITWSTSDGTTEEGELLAENTAATEQDVTFGTAILGAKKLSSKIIRVSNELLQDSGV
                     DIESYLANRIAQRIGRGEAKYLVQGTGTGSPLQPKGLAASVTGTIQTAASAAFTWKEM
                     NALKHAIDPAYRGGPKYRWAFNDATLQTIEQMEDGQKRPLWLPDIAGGTPATVLGIPY
                     VIDQAIDGIGTGKKFIFLGDFNRFIIRRVTYMELKRLVERYAEFDQVAFLAFHRFDCV
                     LEDVAAIKALTGK"
     gene            complement(8684..9286)
                     /locus_tag="ACIYSB_004583"
     CDS             complement(8684..9286)
                     /locus_tag="ACIYSB_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGC5431485.1"
                     /translation="MNDREIRCYSGEVRAEKHDDNPAHIIGYGSVFDSRSELIFGSFR
                     EIIRPGAFDDVLGDDVRALFNHDPNFILGRSAAGTLNLSVDERGLRYDIQAPETQTIR
                     DLVLTPMQRGDINQSSFAFRVARDGEEWYQDEDGVVIREITRFSRLFDVSPVTYAAYQ
                     EAESGVRSMKAWQEARASGALKKAVNERMARERLLTLLNA"
     gene            complement(9279..10505)
                     /locus_tag="ACIYSB_004584"
     CDS             complement(9279..10505)
                     /locus_tag="ACIYSB_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGC5431486.1"
                     /translation="MLLDALFRSEPLENPSVPVTGETAETDNIFARDVYVSPETSMKL
                     AAVYACIYVISSSVAQMPLHVMRKTDDQVHPARDHPLFWLVHDEPNAWQTSYKWRELK
                     QRHVLGWGNGYTWVKRNRRGEVTSLECCMPWETTLLNTGGRHTYGVYNEEGAFAVSPD
                     DMIHIRALGNNQKMGLSPIMQHAETIGMGMSGQQYTSAFFNGNARPAGIISVKNELNE
                     QSWSRLKNMWQRAVTGLRSQENKTMLLPAQLDYRALTVSPVDAQIIDMTKLNRSMIAG
                     IFNVPAHMINDLEKATFSNITQQAIQFVRYTMMPWVANWEQELNRRLFTRTERAAGYY
                     VRFNLTGLLRGTPQERAQFYHFAITDGWMSRNEARAFEDMNPVDGLDEMLVSVNAANP
                     LNNFKDTKGKEEKDDE"
     gene            complement(10495..10656)
                     /locus_tag="ACIYSB_004585"
     CDS             complement(10495..10656)
                     /locus_tag="ACIYSB_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431487.1"
                     /translation="MKQLITDMTGLIGFGLLTAGVYLYAGLPASLILSGCLLLLYALV
                     VSMRRKHAS"
     gene            complement(10653..>11264)
                     /locus_tag="ACIYSB_004586"
     CDS             complement(10653..>11264)
                     /locus_tag="ACIYSB_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGC5431488.1"
                     /translation="DLAHKIDICAAAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAE
                     LYRKWAEMDKLILTDGDVIDHGQIKSDLLEWTGGENLRELGFDPWSAMQFSLALAEEG
                     IPLVEVPQTVRNLSEAMKETESLVYAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNK
                     CTPEAKIDGPVALFTAMSRLLVNGGDVNDFLSTLDPDEDLLIL"
BASE COUNT         2636 a         3323 c         2514 g         2791 t
ORIGIN      
        1 gccatccgga aataatcctc cggctcaccg gagatattca cgcagggaaa atatctttcc
       61 ccctccggcg ttctcaccac gaagccgcac gactccgctg gcgcacatcg ccgggcgtgc
      121 gccagaatcg ctgattctgt ctctgtcatg gaattactgc gaaagtttgt gaatggaaag
      181 aaaaccgcca aagttgccga cgttattgcg aaacttacag ccgctcaggc atttgctgca
      241 tttatccttc gtgatatcgg acgtcggctg atcatattca tccgcgaccg ccggaccgct
      301 ataaccgcac tcatcgccgc gataggtcca ggtgcaggtg ttggccagca tgatacgtcc
      361 cggaaaaaca gcgccgtccg tttccgtcgg cgtggccagt acaaaggagg cactcaccgc
      421 gctcagttcg ctgcactgct cgatgcgcca gcggctgatc acctcctgct ccggatcggc
      481 gtcgctgttt ccgttgacga agttcaccgc atccagaaaa cgggcgtaaa ccttacgcct
      541 gaccaccgtt ccgccgacca gactctgcat atcttccacc ataccggtga ccataccgtg
      601 cagattagag actttcagtg ttggtctggc actggctcct ttgccgttca tctcaaatcc
      661 gctcccctga atgggatacg gctgatactg ccgctcctgc caggtgaccg cctcaccttt
      721 ttcgttctgc tcattacaga aaaaataacg ttctccaccg acctctgtca gatcgatttc
      781 ccagagcacg accagtgcgg attgctgcga ttttgtacac tcattaagtg tttgcggctg
      841 tagatcctgc atcagtgagt aacctcttca aaggtacagc taaaatcggt atacatggca
      901 ttatccgaaa tactccactc cctgcagaca acccggacag ttctgttgta ttttggtggt
      961 cgccataaaa aagcacgaac cccggcatga cgggataaaa aactgtccag tgctgcgcgg
     1021 gaatattcat ctgtgacacg aaataccggt ttaaacgttt tcagatctgc attcagacca
     1081 cccgcccttc gctgttcata tccgtcaccg aactttaccg taataacaga tggctttcgt
     1141 gtcgtctcca tcccctccct ggggagccac ctaaaaattt taagttcacc cactcaacaa
     1201 tcctccatcc cgacgcgatg actgcataac tgacacaacc ctgctgtcga tcagatccac
     1261 cagtcccctg gctgagcgcg catctatctc gccattgctc ccctgattct gaatgctgat
     1321 gtgatacacg ggagaataaa caaatccacc gccaccattc acattgccaa tggctctgac
     1381 tccaagagag ccgtccgctg cccgagtcag tggcatgata gcttcaggcc cggcctcgcc
     1441 catcagcccg gcacctttcg caaaagcaaa atacgtcggg gtatccacaa tggtgttact
     1501 gtaagcactc agatttgccg atgtataaac accacctttt gcgtttgcca ctgcaccgga
     1561 aagccagtcg ccgactgtac caagccatcc tccggcaccg gacatgcttt tggaaagtga
     1621 cttcagcccg ttaacgatgg cagcgttcat cagaattttt gaaacttcct ggagaattga
     1681 actcccccag tttctccagt ccacaacatt tccggccagt gcatcggaaa tatttgatac
     1741 cagcccgtcc atagtggaaa cgacagcatc tgccgcctgc gaagcataat cggtggcact
     1801 gtctgcccag ttcgtcagtc cctcctggag tccggcattc cagttactgc gtaaagcatc
     1861 agcctttgca taataatcct gctgatcgct gagacgctct tccagatatt tttcattcag
     1921 agatttttcc tgtttccaca gggcttcttc aatttctccg gcctgatact gtctcagtag
     1981 ctcgttattt ttctgctcaa actcctgccg gatgctccac atttcctgaa gtcgttcacg
     2041 catccgtgag ccttcaccat atcccagtaa ctgagcctca tttgatgctc tgacactggc
     2101 attactgtcc gccagactgc tctcatacgc agcaagctgc tcacgaatct ttttctggtc
     2161 gatgagtgct gcattctgca aaagcgtttt tttctgcgct tctgacaggg ttgataattc
     2221 gcccttgctg acctgatatt tcatcttagc cagttcagta ttctgccctg ccagtgctat
     2281 ttgctctttt tgctgtttaa tcagtcgctt ataagtatct tctgtttttt ccgcttcggt
     2341 ctttttatgc gctttgggtt tatttgcctg gttatttcgc catgcatcca gtgagttatt
     2401 tatataattc agtcttgctg tctgatacgc ctctcccaca aagccgagat catccgcagc
     2461 ataacccagg cgggcacgct cacgggcttc ccccttcagg cgggacagag ccagttcgcg
     2521 ctcgctgtta ttcagtgcgg tctgctgttt atcatccagg gttgcctgtg gtagccgtaa
     2581 cggtacattc accagcccct gtcgctgctg aagtaattca ttaccgagcc cgagaaggcg
     2641 attaaactcg gtatgctgcc cattcatgat caacagggac tgatacgctt tgttttgttc
     2701 cgctgcctgt tgacggatca acgccacccg tcgctcctcc agcccggcaa gcacatcctg
     2761 aatggattgc gctttgccct gcatttgagt gaggcgagac tgttcaactg ccagttgatt
     2821 tgttgcttct gcaagccctt ctgtgacagt ttttaccgac gtcatgtggt taatcataaa
     2881 accgttatcg gttgtccaac ccggatttgc cagcacatac tgatagccag caattttttc
     2941 ctgtaaggat ttaatcttac ttttctgctc gtcaattaac ctgttttgct ccttcagtgc
     3001 ctgtcgcgtc ttttcctcat tatctgacgc ttcaggaagc gacattgccg acgttttctg
     3061 gcgaatttcg tcgattgttg cggcatactg gcgtgcagat tctctggcct gctcctgatt
     3121 ctgatacatc gtgtaccagg ccgcagcccc cagcatgaca agccccggca caccaccaac
     3181 cagccccagc gcgccactta ataaacgact ccccactgac gtaacattat tcagcgttgt
     3241 ctgtgccgct gttctggccg caatattacg ggtaagtgac gcctgggcag ctgtcagctt
     3301 cgcttctgct gcggcctgcc tttcggtacc gcgagcagca acaaccgcct gttgcgcacg
     3361 ataaaccgcc gcacgcgccc tggcggttgc tatctgtgtc ccccgaagtt gcacttcagc
     3421 aagagccact tcgtttctgg ctgcagtaat taatccggca gttgcagatc cagcagacga
     3481 cgccatattg ccaaaatatc gggctacccc gacggcaacc agagcaccgg cagcggttgc
     3541 cacggtgtca atattgcctg caataccatt cagcacaccg gagagcgtct tcgtcactcc
     3601 gcttgcctcg ttcgcaccac caacccaggc cataaaggcg ttttcaactt tggttgcaga
     3661 ggatgaaacc gtatcaggca ttgccgcata ttcatcacgt aatgccccaa gctgactaat
     3721 cagtgcagga acaaccttat cggcggtcaa ctttccgtta tccgccatgg ccttcagatc
     3781 cttacgggca actcccattc ccgcagccag agcacgaata acacgatcgc cactctcatt
     3841 caccgagtta aactcttcac cgcgcagcac tccctgcgcc agagcctgac tgaactgcgt
     3901 gatcaccgaa ctggcttctg ctgtactggc accggataat ttcaggcccg tggagatcgc
     3961 ctcggtgact ttcagtacct cctcagaact gtaaccatac tcccgcatgg aagctgcaga
     4021 gcgggcaaaa aggctggcgt tatcagaaaa agccgtcccc gttctctggc tgatcgccat
     4081 taattcacgc tgtgatgact gaaaatcatc actggactgt gaggcctgct tcagacgggc
     4141 atttactgaa ttccactcat cggcgagaga aataagatga ccggtagcaa aagccccggc
     4201 aaatgccccc gccatgttca gtgccgaaga tttagctgta tttatctgat ccgtcacttc
     4261 tgccagtgca cgccgcattt catgggatac agcagcggac tgtcggcctc cgttctgcat
     4321 ggtacggtag taatcctgcc ccatacgcga agcccgggag atctctgact ggaatgaccg
     4381 ggaatttgcc gagattttaa taatcagttc acgtaatgtc gccacactca ctctccggac
     4441 gaaaaaaaac cgccgaagcg gttatgttga ctcactgaga cacggttaaa agcgcgtttt
     4501 ccagtccggc aaatggatct gaggcgcctt ctgtcagctc ttgttcccac tgaagaagcg
     4561 cgtcattcag tggtactttg acaccctgcg caccgtaaac cgcagaaact atctgggcag
     4621 cccggatatc agcccgttcg tcccccagcg ggctgaacct gtcaaattct gcccacatca
     4681 tgatttctga tgcggacatt tcccggcgta attctgacaa tgtgcgcccc atcctgagcg
     4741 ccagcatcat cagaaaacgc atccccggaa gcgctacttt tttttaacct ctccggcatc
     4801 actgatcagt tccagtgact gacgaagaag ccgcgcatgc accgggccat acacggcaat
     4861 cacctgttca cgatcatcct ctgaaaatac aggttgcagt ccggtatcac acagaacatc
     4921 gatgaacagt tcaacatccg ctttcagatt tcggcgggcg cgctccgcaa cggataacgg
     4981 tgtctcatca tcttttgctt taacgatctc ctgccagcgc aaccaggctt ctgcagaagg
     5041 ttcccgtaac acaaccgttg ccccttccca ttcaggcaca tcaacagttt tatggcgaaa
     5101 ccccgacatc gttgccagtg ccagattacg gatattttta gtcatcacat ctatcctcat
     5161 taactgacgg taacagtgca ggaagtggag gtcaccttgt taacagggct tgctgaatca
     5221 gaaatctcgc aggtatacgc accggcatca ccggatactg cggatgcctt actgaacgtt
     5281 gccgccgtct gtccggcaac aggagaacca cctttcttcc agacataaga ataaggcggc
     5341 acaccaccgg cagcctcaac caccatttcc agtttattgc cggtagagac tttcagcgta
     5401 ctttttaagt caagcttaac tttcagcaac tgtgtggtca gcacaggctt acctttcagg
     5461 cgaagggaaa atgtggcagc caccacgccg ttagtacctg cagaccaggt atgctgacgc
     5521 acctctgcca taaaggtaaa tccgttgcct gacggaaaaa taactttaaa gccatacgtg
     5581 gcgtcattgt cataggcact gcgcaacgcg ttctgggcag cattgacata aaaatttcct
     5641 gacatggaaa tctctgaagc agccccaaga ccgttgatat tctcctgctc aacagaacac
     5701 agcgtggtga cgtcaatgtc ctgtttctgc ccggcggtaa actgaacctc tttaatcgta
     5761 cagctcagtt caagaaaact ggcagaatcc agtgtttctt tccttaccgg tacggacgaa
     5821 atcataattt tcgtcagttg cgaacgttca aaattcgagg gcatactcgt ctcctgaaaa
     5881 taaaaaaccc gccagcggcg ggtgggtaaa atcattaacg acctcaggct attacctgaa
     5941 attcaagcgt ggctctgctc agacgggaat caggatcata accctgcgtt ttagaaataa
     6001 cggagggtgc aagttgcctt accgcatcaa gcgcctgctc acggatatta tctgcgtcat
     6061 caggtatcct tgcccagaca tcgatctgca cggtaattct ggattcagcc tgaccatcaa
     6121 gcacatcaga tgcggtatca gacaccacag aaaacaccag ccacggcgga gataccgcag
     6181 gctttccctc cgtcagcggg accacataag ggtaaacctg tcctccggcc agctcagaga
     6241 gccgggaata cagtgcggcc tccctcattt acttaatacc tcatcaatag cctgattcat
     6301 tctctgtatg gcaacccgtg ccgccagttc ctcagtcgta tcaaacgccg gacgaatgaa
     6361 cggatgtgcg ggcatattta ccgtgcccag ttcgacaaag cgccagtaaa acgcattccg
     6421 gggatcgctg gctttcatgc tgttatcact gtttccggtt cgcaggttcc gtccgcgaat
     6481 gtggacaccc gagataattt ccccccgacg ctttgaacgc tgagtgagaa caaccacatt
     6541 tttcttcagt ttcccggttt gctccggcgc acgttcaaca accgcatccc gcataatttc
     6601 agcaccggca cgggtggcat cgcgcagtac cttattgttt tctgccctgc tgagcgtctc
     6661 cagatcccgt gcaatatctg ccaggccgga aaaatcaaga ctgaaatcca tcacacattc
     6721 cccttctgag agcagagtat ttcaatccgg gtggcacgtg catccggtat cggcggaccg
     6781 tctatactca gaatcgctcc tttgaatgca ccagtcagca ctttcagaca tgaagttgct
     6841 gtcacatcgc gccggaatct catccagact ctcacggtag cctgagcggt ttccgcaccg
     6901 cccgatatac gttcccgtcc actgatccct ttaacctctg cccatacggt ggctccctcc
     6961 gtcatcgtct ccaccgggtg ccctgacgga gagcgaacgg tggtggcatt cagaataacc
     7021 acacgatcgc gtaatcttcc cgcctgcatg gctcctccta caaaggaata aaacgataag
     7081 gctccagaag agaggaaaaa ccaaacggta tgggagcctt gctgacatct gaggaatttt
     7141 cccggttttc gtaccagtgc ccgaccagca acatgagcgc cagcaaaaca tcatcagcaa
     7201 taagcacccc ttcaggatca tcctccggca ccgtctcctc atagagctta cggttgataa
     7261 agttttctgc cttacgacag gcagcccgga aatacagcat cagtaactca tcatcagttg
     7321 catcatctgt atcaatacgg cactgcgtcc tgagtttttc cactattgct gccatcagaa
     7381 actcctgccc gcaacactgt gcgggcataa aaaaaccgcg tcggcgcggt ctgtaactga
     7441 acaacgtgtg gttatttgcc agtgagcgcc ttgatggctg ccacatcttc cagcacacag
     7501 tcaaaacgat ggaaagcaag aaatgctacc tgatcaaact cagcataacg ctcaaccaga
     7561 cgtttcagct ccatataagt aacgcggcga atgataaagc ggttgaaatc ccccaggaaa
     7621 atgaattttt tcccggtacc aatcccgtca atagcctgat caataacata agggatcccc
     7681 agcacagtag ctggcgtacc gcctgcaata tccggaagcc ataacgggcg tttctgtcca
     7741 tcctccatct gttcaatagt ctgcaatgtg gcatcattga atgcccagcg gtatttcggc
     7801 ccaccacgat atgccggatc aatggcatgt ttcagggcat tcatttcttt ccaggtgaaa
     7861 gcggcagaag ctgcagtctg gatggttccc gtcaccgacg ctgccagccc ttttggctgt
     7921 aacggtgatc ccgttccggt cccctgaacc agatattttg cctctccacg accaatacgc
     7981 tgggcaatac ggtttgccag ataagattca atatccaccc cactgtcctg gagcagctca
     8041 ttggacacac gaattatttt tgatgacagc tttttagccc ccagaatagc agtcccgaac
     8101 gtcacatcct gctccgttgc ggctgtattt tccgccagca gttcgccctc ttcagtcgtg
     8161 ccgtcagacg ttgaccaggt gatatcctgc ccggttgatg tggtcagaag ttgtgccaca
     8221 ccggcaatcc cgccataagc cttcatggcg tcaatgattt tgttacgcat ctgcgtgggc
     8281 accgtatatc cgccctgaga atccgttgtt acaccctgag cccgcagttc acgcatcaga
     8341 ttacgctctt cagcattcag ttctgcaaat ccggcacgca gaaaacggtt aaatgccgca
     8401 gcacgcttct cttccaccgc ctttttcccg ttctccgcct cattattctg gcgctcttcc
     8461 ggcccggact catccacata tgcctgatcc tgacggcgca actcttcttc acgggcgatt
     8521 tgctcatcca gcgcatccag ctcagctttc gccctgttcc actctgcccg ttgctcatca
     8581 gtccatgcgt tatcaccaat tttttcatgc agtgcacgca tatcctttgc aatggtgttt
     8641 cgtttttgcc tcatctcatg aagtttcatc gtcagtagta tccttatgca ttaagaaggg
     8701 tcaaaaggcg ctcacgcgcc attcgttcat taacagcttt cttcagcgca ccactcgccc
     8761 gcgcttcctg ccaggctttc atcgagcgaa cgccagattc tgcctcctga taggccgcat
     8821 atgtcaccgg gctgacatca aacaggcggg aaaagcgggt tatctcgcga ataacaaccc
     8881 cgtcctcatc ctgataccac tcctcgccgt cacgggcgac acggaaagcg aaagatgact
     8941 ggttaatatc tccgcgttgc atcggggtca gcaccaggtc acgaatggtc tgtgtctccg
     9001 gagcctggat gtcatagcgc aatccacgtt catcaactga aagattcagc gtgcctgctg
     9061 cactacgccc aagaataaaa ttaggatcgt ggttaaacag tgcgcgtaca tcatcaccaa
     9121 gcacatcgtc aaaagcgccg ggccggatga tttcgcggaa tgaaccgaat atcagctcag
     9181 aacgactgtc aaacaccgat ccataaccga taatgtgcgc cgggttatcg tcatgcttct
     9241 cagcacgcac ctcaccgctg taacaacgga tttcacggtc attcatcgtc cttttcctct
     9301 ttgcctttcg tatctttaaa gttattcaac ggatttgctg catttacgct gaccagcatt
     9361 tcatccagac cgtcaaccgg gttcatgtcc tcaaatgccc tggcttcatt ccggctcatc
     9421 cagccatctg taatggcaaa gtgatagaac tgcgcacgct cctgtggggt cccacggagc
     9481 aaccccgtga ggttgaaacg aacgtaatac ccggcagccc gttctgtacg ggtaaacagg
     9541 cggcggttaa gctcctgctc ccagttcgca acccagggca tcatcgtgta gcgaacaaac
     9601 tgaatcgcct gctgtgtaat attcgaaaac gtggcttttt ccaggtcatt aatcatgtgc
     9661 gccgggacat taaaaatccc ggcaatcatc gacctgttca gtttggtcat atcaatgatc
     9721 tgagcatcca ccggtgaaac tgtcagagca cggtaatcca gttgcgcggg caggagcata
     9781 gttttatttt cctgactgcg aagccctgtc accgcccgct gccacatatt tttaagcctg
     9841 ctccagctct gttcgttcag ttcatttttc acagaaataa taccggcagg acgcgcgtta
     9901 ccattaaaaa aggcactggt atactgctgg ccgctcatcc ccatgccaat ggtttcagca
     9961 tgctgcatga tcggactcag tcccattttc tgattgtttc ccagtgccct gatatggatc
    10021 atgtcgtccg gacttaccgc aaatgcgccc tcttcgttat acaccccgta ggtatgacgc
    10081 ccaccggtgt taagtaacgt ggtttcccat ggcatacagc attcaagact ggtaacctcg
    10141 ccacgacgat tacgttttac ccacgtataa ccattgcccc accccagcac atgacgctgc
    10201 ttcagttccc gccacttata gctggtctgc caggcattcg gttcatcatg aacgagccag
    10261 aacaacgggt gatcgcgtgc cggatgaacc tggtcatccg tttttcgcat cacatgcagg
    10321 ggcatctgag ccacactgga tgaaataaca taaatacagg catagacagc agccagcttc
    10381 atggatgttt ccggactgac atacacatcc cgggcaaaaa tattatccgt ctcagctgtc
    10441 tctccggtta ccggaaccga gggattttcc agaggttcac tgcgaaacag agcatcaaga
    10501 agcatgtttt ctcctcatgg acaccaccag tgcataaagc agtaacaaac agccggacag
    10561 gatcagagac gctggcagac ctgcatacag ataaacgcca gcagtgagca gaccgaaacc
    10621 gatcagcccg gtcatatcag taataagctg tttcacagaa ttaacaggtc ctcatcagga
    10681 tcaagcgtgg acagaaagtc attcacgtcc ccgccattta ccagaaggcg gctcatggct
    10741 gtaaaaagcg caacagggcc gtcgattttg gcttccggcg tgcatttatt cgggaagata
    10801 ttgtcgtttt tgtccggttt caccgtaacg ttagacatca tccagttcat gaccgggtga
    10861 ttgctgtgat ggaaacgccc ggcatagacc agtgattccg tttccttcat ggcctctgac
    10921 agattgcgaa ccgtctgcgg aacctccacc agcggtatcc cttcttcagc cagtgccagg
    10981 ctgaactgca tcgcgctcca cgggtcaaat cccagttccc tgaggttttc accaccagtc
    11041 cattccagta agtcactttt tatctggcca tgatcgataa catcaccatc cgtcagaatc
    11101 agcttatcca tctccgccca cttccggtaa agttctgcct gctgccgcga gcatcgttcc
    11161 agccgtcctt ccggaagcca gaatttaaaa tcggcatgaa catgcccgtt atccgttcgc
    11221 cagagttttg ccgctgcaca gatatcaatc ttatgagcaa ggtc
//
LOCUS       DBBGLQ010000130        11259 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.130, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000130 DBBGLQ010000000
VERSION     DBBGLQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.130"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..361
                     /locus_tag="ACIYSB_004587"
                     /pseudo
     CDS             1..361
                     /locus_tag="ACIYSB_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(645..1244)
                     /locus_tag="ACIYSB_004588"
                     /pseudo
     CDS             complement(645..1244)
                     /locus_tag="ACIYSB_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1232..2411)
                     /locus_tag="ACIYSB_004589"
                     /pseudo
     CDS             complement(1232..2411)
                     /locus_tag="ACIYSB_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611657.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2808..3839
                     /locus_tag="ACIYSB_004590"
     CDS             2808..3839
                     /locus_tag="ACIYSB_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGC5431489.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGCWINWFTQSGVEGLKSLPAGRARLWSFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITDCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSTEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYDLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYLRVKTITLIVDNYIIHKSRETQRWLK
                     ENPKFRVIYQPVYLPWVNHVERLWQALHDTITRNHQCRSMWQLLKKIRHFMETISPFP
                     GGKHGLAKV"
     gene            3838..4331
                     /locus_tag="ACIYSB_004591"
                     /pseudo
     CDS             3838..4331
                     /locus_tag="ACIYSB_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028172.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4556..4642)
                     /locus_tag="ACIYSB_004592"
                     /pseudo
     CDS             complement(4556..4642)
                     /locus_tag="ACIYSB_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4996..5268)
                     /locus_tag="ACIYSB_004593"
     CDS             complement(4996..5268)
                     /locus_tag="ACIYSB_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431490.1"
                     /translation="MDNRTITLQELLEYMSMLRDDMNALTVAFSYLASSLPKEQMIQI
                     LAFIHFESNNSRWSSEQQKSFKWLASLLEGCYTVHTNIFDIPSKNH"
     gene            5593..7680
                     /gene="iha"
                     /locus_tag="ACIYSB_004594"
     CDS             5593..7680
                     /gene="iha"
                     /locus_tag="ACIYSB_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGC5431491.1"
                     /translation="MRVTTLASVAIPCLGFSVSSMAAEDVMVVTASGYEKKLTSAAAS
                     VSVISQEDLRSSQYHDLGEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSSDVTPNGYAAMNTGFMPPLAAIERIEIIRGPMSTLYGSDAMGGVVNIITKKNA
                     DKWRSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSLSLQVRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNAGGRLDWKATDKDVLWIDMDIARQRYDNRDGQLGSLTGGYD
                     RTLRYERNKISAGYDHTFTFGAWKSSLNWNETENKGRQLVRSVLQRDKWGLAGQPREL
                     KESNLILSSLLFAPLGESHKVTVGGEYQNSSMKDGVVLASTGETFRQKSWSAFVEDEW
                     YITDAFALTAGSRYERYEVFGGHFSPRAYMVWDVTDAWTLKGGVTTGYKAPSMGQLHK
                     GISGVSGQGRTNLLGNPDLKPEESVSYEAGVYYDNHAGLNANITGFMTEFSNKIVSYS
                     IDDNTRSYTNSGKARTQGVEFAGTLPLLSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVTSWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWT
                     VLDAGMSWKVTDALMLNAAVNNVLNKDYSDVSLYRAGSGTLYAGDYFQTGASTTGYVI
                     PERNYWMSLNYQF"
     gene            8615..9010
                     /locus_tag="ACIYSB_004595"
                     /pseudo
     CDS             8615..9010
                     /locus_tag="ACIYSB_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
     gene            9007..9981
                     /locus_tag="ACIYSB_004596"
                     /pseudo
     CDS             9007..9981
                     /locus_tag="ACIYSB_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
     gene            9988..10449
                     /locus_tag="ACIYSB_004597"
     CDS             9988..10449
                     /locus_tag="ACIYSB_004597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431492.1"
                     /translation="MMMKMKTGMVALSVMLYCSIPAMADLLSLPKLNDSTFGYGKSVD
                     GAVSDKLFYTLGGGSVISQLATRSNMQKLGMDLGWSSDLMCGNFDLKTTVGNHLNGLT
                     DGFKNLMGDVIQGATGAAASLPAMVIQRANPGLYDMITNGVLQANVAFDKA"
     gene            10724..11116
                     /locus_tag="ACIYSB_004598"
                     /pseudo
     CDS             10724..11116
                     /locus_tag="ACIYSB_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681776.1,RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         2910 a         2520 c         2978 g         2851 t
ORIGIN      
        1 aacgaatcat gacaatcccc ggtgttgggc cgatgacggc cagcctgcta tcatcccagc
       61 ttggtgatgg taaacaattt tcgtgcagta gggattttgc agcatccaca ggacttgttc
      121 cccgacaata cagtaccggt ggtaaatcaa tgctgctggg gatcagcaaa cgagtcggtt
      181 acttgttctg tacgcccgct cgttcatgat gcggctggag catcaatagg gcagagttgc
      241 tgaatgggtt caaaagcaac tcgacaataa gcattcaaat attgtcgcct gcgctctggc
      301 aaataaactg gcccgaatag cctgggcaat cactgctctg cacaaagaat atcaggcctg
      361 atatagatac tgacaaaact caattagctt attttaaaaa ataagataca agttacctgt
      421 ctggttttgc gaagactgat tattgatgac atgaacggca aatagacctg attaaaaacc
      481 tgacagaaaa aatggctcac tgaagccgag ggtcttttta ggattatcag gtgcggaact
      541 catcgaggcg cgggtctcct tgtccacaga gacgccggat agatttaagc aagcccacca
      601 ttaatcaaaa atcagtgtag caaaaacggg ggtgaccata gattttttat gatttcctcc
      661 gcttttttca gaatatccag ttccatctgc cggcggcgta tttcctgatt cagtcgggcg
      721 acttcctccc gcaacgcatc gcgttctgcc tccagggaag gttcgttatg tttacgcata
      781 gtctggtaag cactgttgcc gataatttca tctttccatt tatacaatac cgcacgactg
      841 acaccaatac gcctagagat ttcactggct gatatttgtc gtgtacagaa tgccatgaca
      901 gcctgacgtt tgacttctgg ttcaaagggc gcaaccggac tggtcttact ggtaaatata
      961 cgtcgtctgc cggggtaacg ttcattaacc cagcgagcga gcacatcggt acagggataa
     1021 cccagagttc tactggtaaa cgctaggcag cagccgtggt taagataatg ttcaacggca
     1081 acttgttttt gctcatctgt gtaacggggc ttatggcgaa tggattcttt tgctccgcca
     1141 cctgattcct atgaacggat ccaacgccgc agatttcttt tcgaagggta gcccagttcg
     1201 cgtacgacgg gagccagctt cttaccattg actagtcttg gtataagttg acacttttct
     1261 gtctgtaaaa cgcctgatga acgtcatagg gcgttttgta tttcagagcc aggtgtggcc
     1321 gctcatggtt ataaattgcc acggactctt ggaccatttc ccgggcctga gccaggtctg
     1381 caggacgcga gagtaaaaac tcatttttca gggtaccatt tattcactca gccaatgctg
     1441 gtagcagtca taaccatcag tcattgaaca ggttatttca tttctttcat gaactgactg
     1501 atagagcgct gaacagtatt gcagcccctg ccagaatgat gcaccagggg actcgtcgtt
     1561 tttctcagct tcagcacctg tctgaacgct tttaccacat tttctgtctg caggttttcc
     1621 ccaacatggt agcccataat ttttctggag caggcatcgg tgaccagact cagataggcc
     1681 gaactattgc gcagtggaag gtaagtaata tcggcgaccc ggacctgttc tggttcaaga
     1741 gcagtaacct gttcagggcc tggtttaagc aggtttggat gtcggtagaa gcgatgatgg
     1801 ctgttggtag ttttgtgata tgaccgtttt actggcacca ggagtcggta ttcatcgggc
     1861 aggttaaaca ggcggtctcg cccgatattc agccttttat cggcctgagt gttcagcaga
     1921 tagtgcagct tacgcgcacc gattcggggc tgacgacatc tgacacgggc aacaaaatca
     1981 agaacctgag catgatgttc ctgccgtttt ctgcgccttg cattgtcctg ataccatgcc
     2041 tgtctgctgt gtcccaggaa cagactgcac gcgtcacagt tattttgggg accggacttt
     2101 gcgcgaggac ttgccgggcc gctttttacg atactcaccc cgtaatcatt tttcaggaca
     2161 ttgataactg actcaaaaaa cgtagctttc tggttcgcca gctcaagcta cgtttcaagt
     2221 tccctgattc tttgctcggg tgtgagcggg atagttgtct gagtcacagg cagtttcctc
     2281 ttcaccagct caggaagtcc aggatattta cgtaaccaga caagaacggt ggagcgcccc
     2341 tgaatgccat accgctgctg ggcctgttta taagtcatct cgcctttttc aacctgctca
     2401 acaacagcta ttttaaagga tagaggataa tcacgttggg tgcgtttgag ctaagtcatc
     2461 attacgttct ccggatttaa gtaggaacgt gtcaacgcta ttcaggacgg gtcacatatt
     2521 tgaaataaag ctcaatggcg cggatttttt cttcttcagt aaacatgttt catctcctga
     2581 gagtccagga aatcgtccgc atccccatta ctgtcttata tcattggcgc tgacagctat
     2641 tctgctgagc cttgaggatg cataaacgag ttaagagctt gtatgtgcag aacgatagcg
     2701 gaactaaata gctgcgcgga atagtagatc actttgaggg agctcagcct ggattgtgcg
     2761 atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg ccgatcatag
     2821 caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa acacacgata
     2881 aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac cgtgtcagcg
     2941 acgttgccag aacgctctgc tgcgcccgtt cctctgttgg atgttggatt aactggttca
     3001 cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgagcccgt ctctggtcgt
     3061 ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc gactttggct
     3121 accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag ataaccgatt
     3181 gccagttaaa tgccggaacc gttcgccgct ggttgccgtc tgcggggatt gtgtggcgaa
     3241 gggctgcgcc aactctgcgt atccgtgacc cgcataaaga tgaaaagatg gcagcaatcc
     3301 ataaagcact ggacgaatgc agcacagagc atccggtctt ttatgaagat gaagtggata
     3361 tccatcttaa tcccaaaatc ggtgcggact ggcaactgcg cggacagcaa aaacgggtgg
     3421 tcacgccggg acagaatgaa aaatatgatc tggccggagc gctgcacagc gggacaggta
     3481 aagtcagcta tgtgggcggc aacagcaaaa gttcggcgct gttcatcagc ctgctgaagc
     3541 ggcttaaagc gacatacctt cgggtgaaaa ccatcacact gatcgtggac aactacatta
     3601 tccacaaaag ccgggaaaca cagcgctggt tgaaggagaa cccgaagttc agggtcattt
     3661 atcagccggt ttacttgcca tgggtgaatc atgttgaacg gctatggcag gcacttcacg
     3721 acacaataac gcgtaatcat cagtgccgct caatgtggca actgttgaaa aaaattcgcc
     3781 attttatgga aaccatcagc ccgttccccg gaggcaaaca tgggctggca aaagtgtagt
     3841 ggtattaggc gcagctattt agatgatctc tttaacgatc tcggtcgtag tataaacttt
     3901 tttgataaat aaatagagac ctgtccaggc aatctgaatg ctgtcagcgt attgcaaaag
     3961 ctaaaggcat tggtcctgaa attgtcacga gcgttgttgc tgttattatt ggcaaaggca
     4021 ctgaatttaa gaatgatcgt cactgtgctg catggcttgg tctgcttcca tgcctgcact
     4081 agagttgctg caggtaagtt ctcatgaata taacgaaaaa aggcgacaag catctgcaaa
     4141 cactttttat tcatggcgtc cgtgctgtcg ttagagttgc tacgaataat aatgatgtat
     4201 acctaaatca gtgggttaag cagttaaagg agagtcgcga atttaataaa actactgtgg
     4261 agattgctaa caaatacgcg agaataatct ggtcgatgct gataaatgat acagaatatc
     4321 aggcagtgtg aagttaatct ctttcagcaa aagttgcagc gtactgagaa atggtgacgg
     4381 gttgcacctg cacaatcgga acctgatttt tgtactggcc tcagaggtcg tccagttgta
     4441 gaggcgattg tgtgtggatt ccctatttgg gcacaggttt tcctgatgcc ggatagatgt
     4501 aagcaacaat ccaaaaccag aatcagtgct tgcaaaactg aggtagtcaa tagatgtagt
     4561 aagtggcttc agaaataccg gcttcccgac aaacatcttt aattgttcgt ccggtttcaa
     4621 ccgatttaat tacagcgatg atctaatact cagtaatacg ggctttacgc atagtgattt
     4681 tcttcgttat cagattgatt attccggatg atctctaaat atgaatggag cgattatgcg
     4741 ggatacttac agatggctct cacttaatga gagtcttctt tcaaacaaca aaaacagtaa
     4801 attgggtaga atggggaact taactgcatc gttaattaat atatggatgc gctgttgtaa
     4861 aatatagatg tatcggggat aataatactt tactctgttt ccagcgagta tgtttggttt
     4921 gctggacaaa gcgcctcata aagtcagagg cgctcaaagt agaaatatat ttcagaggga
     4981 atgtatcata atgtactagt ggttctttga tggtatatca aaaatgtttg tgtgaactgt
     5041 ataacagccc tccagtagtg atgctagcca tttgaaagat ttctgctgtt ctgaagacca
     5101 tctggaatta tttgattcaa aatgaatgaa tgccagtatt tgtatcatct gttccttagg
     5161 aagcgatgaa gccagatatg aaaatgcaac cgtgagagca ttcatgtcgt ctcgaagcat
     5221 tgacatgtac tcgagtaatt cctgcagagt aattgttcta ttgtccataa aactcctcat
     5281 tattaattat attcacctgt ctgatgtacg aaatatccga tagtgtatga ttaacatatc
     5341 caaggacaag aaactgataa agattggata aatacccata gtgcaaaaat ccgatatgga
     5401 tcgtcaacga ttgctatccg ttactttccc ctagagaaaa gaacgtcctc aactgaaatt
     5461 cggggtatag tagaacctta tgtaatattt tgtaattttt tactttttta tgtaaaaacc
     5521 aaaagcaaat cattctcatt atctcttgcg tttgtttatc ttctctctga ttaatataat
     5581 cggagagaag atatgcgagt aaccactctg gcttcagtag ccatcccttg tctcgggttt
     5641 tcagtcagca gtatggctgc ggaagatgtg atggtagtca cggcgtctgg ctatgaaaaa
     5701 aaattaacca gtgcagctgc gagtgtttca gtgatcagcc aggaggattt gcgatccagc
     5761 cagtatcatg atctggggga ggcactgaga tccgtagagg gggtggatgt tgaaagcggt
     5821 acgggtaaaa caggtgggct ggaaatcagc atccgcggaa tgcctgccag ttatacgctg
     5881 atactgatcg atggtgtccg tcagggcggg agcagtgacg taaccccaaa cggttatgct
     5941 gccatgaata cgggttttat gccacctctg gccgccatcg aacgtattga gattatcagg
     6001 ggccccatgt ccactctgta cggctctgat gccatggggg gcgtggtgaa tatcatcacc
     6061 aaaaagaatg cagacaaatg gcgctcttcg gttaatgccg ggctgaacct gcaggaaagt
     6121 aacaaatggg ggaacagcag ccagtttaat ttctggagca gcggtcccct tgtggatgat
     6181 tcactcagcc ttcaggtacg tggcagcaca cagcagcgtc agggctcatc ggtcacatca
     6241 ctgagcgaca cctcagctac gcgtatccct tatcccacgg aatcacagaa ttataatgca
     6301 ggtggtcgtc ttgactggaa agcgacggac aaggatgtac tctggattga tatggatatc
     6361 gcccggcagc gttatgataa ccgagatggc cagctgggca gtctgacggg cggttatgat
     6421 cgcaccctgc gctatgagcg taataaaatt tcagcaggct atgatcatac attcaccttc
     6481 ggagcctgga agtcatccct gaactggaat gagacggaaa ataaaggccg tcagcttgta
     6541 cgtagcgtac tgcagcgtga taaatggggg cttgccggcc agccgaggga gcttaaggag
     6601 tcgaatctta tcctgagctc attactgttt gcacctctgg gggaatcaca caaggttacc
     6661 gtcggtggtg agtatcagaa ctcctccatg aaagacgggg ttgttctcgc cagtactggt
     6721 gaaaccttcc gtcagaaaag ctggtcggca tttgttgagg atgagtggta tatcacggat
     6781 gcatttgcac tgactgcggg tagccgctat gaacgctatg aggtgttcgg aggacacttc
     6841 agtccccggg catatatggt ctgggatgtg actgatgcgt ggacgctgaa aggtggggtg
     6901 accacgggat ataaggcgcc cagcatgggg cagttacata aggggatcag cggtgtgtcc
     6961 ggtcaggggc gtacgaatct tctcggtaac ccggacctga agccggagga gagtgtcagt
     7021 tatgaagcgg gcgtgtatta cgataatcat gccggtctga acgccaatat cacgggcttt
     7081 atgacagaat tctccaacaa gatcgtatct tattccattg atgataatac ccgcagctac
     7141 acaaacagcg gaaaggccag gactcagggc gtggaatttg ccggcacact gcctctgttg
     7201 tcagaggatg tgactctgtc tctgaattat acctggacgc agagtgaaca acgggatggg
     7261 gataataaag gtgcaccact gagttacacc cctgagcata tggtgaatgc gaaactgaac
     7321 tggcagatta ctgaagaggt gacatcatgg ctgggtgccc gttaccgcgg gaaaacgcca
     7381 cgtttcaccc agaattattc gtcactctca gctgtacaga agaaagtgta tgatgagaaa
     7441 ggtgaatatc tgaaagcctg gacggtactg gatgcgggca tgtcgtggaa agtgacggat
     7501 gcactgatgc tgaatgcagc ggtgaataat gtgcttaaca aggattacag tgacgtaagt
     7561 ctgtatcgtg ccggcagtgg tacgctttat gcgggtgatt acttccagac gggggcatcg
     7621 acaacgggtt atgtgatacc tgagagaaat tactggatgt cgctgaacta tcagttctga
     7681 taacaacaaa cccgatataa cgcattataa tacgtaaaga tgctgcctct gtcacctgag
     7741 ggcagtggca gcaacacacg atgcaggtgt ttgatcacca gtgacccgcc gtttactcct
     7801 ttagtatggc atacttctgt tgtcacctgt gaccaggcgc gcgactctgt aaacatccgg
     7861 atgtatgcgt tcttctgaca gttaatatta ccggcaggta gttgacgatg tgtgggcttg
     7921 tgaccgcatc cgggtaataa agaaataatc cgccagcggc ttttattctt atgggcagta
     7981 attaaataca tcgccatgtg ggtaaatata agtgacggaa tgagagtaac aagtgatgct
     8041 ggaattaaga ttttgctttt tgcagtgata gccagagcct tttacagaaa tgtggtatga
     8101 ggtgcatttt ttacgctcac attattgttt tattatcggc gtcaaccagt gatcaggagg
     8161 tgcaacatgc gtatatgtgt ttttggttac aaaacatgtc cttcagtaca ccggatcatg
     8221 catcatgcga caaatctccg ggatgaaaca ggggcagatg tcctctttat tgatgctgag
     8281 aaacatggta tcagtctgaa ggtaatgtct gagcgtgctg gatgtgcagt tgaaaaattc
     8341 ccctgtgttt ttgttgatga cgtctatctc gaaccactgc caccggtgcc gggacaacct
     8401 cattatactt attgttgaaa atcaccactt ggtatgaatc agttccccgg ctttgtgaaa
     8461 ggccgggtat aatcacagac tgtctgttat gcttggcaga tacacttccc cggatcccac
     8521 cgtaattttt taatcccgtt tttaatttat tgctgataaa tgagcgcttt cttcgcacgc
     8581 tgtcagtcag ataatatctg actgacagcg tgcgatgtac cggattttct gttccctgtt
     8641 tttcctgccg ctggcagcgt ctgccggcac aacaatttat accgattctc agcatcttcc
     8701 cgttaatcct cctgatggtg tcagggtggt tctgctggat gcgcctgagc agcttcagag
     8761 ccgcttttgg ggcctgttgc ctgctgatgc gggtgaggct gaatcggtgg tcagggtacg
     8821 tatgaagtct cccgaatggc agacgatgca ggcggaactg gccggtcatt accgcgatgt
     8881 ggcacatgcc tagtcgctgg gcgtgaagaa ataccctgcc gtggtgtttg atgacagtga
     8941 ggtggtttac ggcaccatgg atgtggtgat ggccgggaaa atccgtgctg agggaggagt
     9001 gcagccatga tgtccctgaa atgtcgtctg cgtaccggcg tgctgttact gagtgtggca
     9061 tcactgcccc tggcgtctgt atcactgaac accgccagca ttatcgccag tactgcggca
     9121 ccggactata tcagctggcg tgtcagcggg atttgttact ggctttactg cagcgcgtct
     9181 ggctgtacgg tcagaatttc cgtgaaggtg acacatttca tcccggaagt ggttatttcc
     9241 acctatacgg caccgggtgg taatccctgg aaagagatgt cgctggtcag ccggaccgca
     9301 ggtggcccgg agaatgccat cactgacggc ctgtccgggc tgagtgccgg cgggggaaat
     9361 ccggcggata tgaaaataca ggggaagcgc cgttcagcca ttcgtttcaa atacgcagat
     9421 gcgctgggtc atccggccac acagcttatc ggcgggatgg tacccggatt cagctgtgat
     9481 tcaccggtga caccggccat ggcctatttc ctcagtaccc tggacagcat cgtctagcga
     9541 acgggattac ctgaatccct gtatccagag gcacaggttc ccggcaaacg cgagattggc
     9601 agctaggccg ataaaaacat gtgggggagc gtttatcacc ggagtggttt tgtgatgcag
     9661 accgatgatg accgggccgc ggcggtgggg gctcagcggg tggccgacat tatcacgcgg
     9721 atgggggagt cgcatgtcta cagggaggtt aagggcgtga aacgtgacgg ctagtggccc
     9781 ccggaggcgg tggaggaaaa caccggcacc cgcaatcaca aatggcagcg actgacgccg
     9841 tcagtgtcgc gttcctgtgc cgttttcccg gatggtgaac accaggcagc ggagaacggt
     9901 aatgcagcct ttgcactgtg gcagccttac agctgcttcg aaaagcgcgg gcagcgattc
     9961 cttggcagta cgaattttta aggaaccatg atgatgaaga tgaaaacagg tatggtggca
    10021 ctgagtgtga tgctgtactg cagtattccg gcgatggctg atcttctttc tctgccgaaa
    10081 ctgaatgaca gcacgttcgg ttacgggaaa tccgtggatg gcgccgtatc cgacaaactg
    10141 ttctatacgc tgggtggcgg ctcggtgatc tcacagttgg ccacgcgcag caacatgcag
    10201 aagctgggta tggacctggg ctggagcagc gatctgatgt gcggcaactt tgacctgaag
    10261 accacggtgg gtaatcatct taacggattg accgacggct ttaaaaatct gatgggggat
    10321 gtgattcagg gggcgaccgg cgccgctgcc agtctgccgg cgatggtgat acagcgtgcc
    10381 aaccccggcc tgtacgacat gatcaccaac ggtgtgctgc aggcgaatgt ggctttcgac
    10441 aaggcgtaac tgaactgcca gaacatggca aaacggatga tggacttttc tgatcgcagc
    10501 aaatggacgc aggccagcga tgctggaaga gtacaagaag gtggtgaaca gcggtgacgg
    10561 tgatgcggtg aaaggggacg aggccggacg tcaggccacc gggaaggaag gtaagaaatg
    10621 gattggcggg cagcagcgcg gcggggcagg tcagcccgcc atccggccgg tgcatgatct
    10681 gacagcggct gggtttaaca tgatgaacag tctgcccgtg accagtacgg gaggttacga
    10741 taaagccgac agtgcaggat gtgatgttag gagcgataga aaaacgcttt ggcgataaag
    10801 gaccggaacg agcaatccag tggcggacgg ataacggttc ggcttatacg gctcatgaaa
    10861 cacggcaatt cgccagagag ttgaatctgg agccctgcac gacggcagtc agtagccctc
    10921 agagcaatag aatggcagaa aggttcgtga aaacgtgaat gaagactaca tcgcgttcat
    10981 gccgaaactg aatgtaataa cggcattgca taaccttgca gaggcgttcg agcattacaa
    11041 tgaaaaccat ccgcatagtg cgttaggtta tctctcacca cgggaatatc gacgtcagcg
    11101 gataacgtta acttaagata caatagcagt ctgatattga gggggcaaga acatccgaac
    11161 cacaccagcc tgtgacttat accctgtctc tacgacccag agagtattat tcttaataat
    11221 ctcataatat atacacaata ggaagtatct attaacaaa
//
LOCUS       DBBGLQ010000131        10629 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.131, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000131 DBBGLQ010000000
VERSION     DBBGLQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.131"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..974
                     /gene="ybgO"
                     /locus_tag="ACIYSB_004599"
     CDS             <1..974
                     /gene="ybgO"
                     /locus_tag="ACIYSB_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5431493.1"
                     /translation="SGGAVEKSEAIQPFAVPGNAKPGDKIWESDDIKIPVYCDNNTNG
                     NFESEHVYAWVNPYPGVQDRYYQLGVTYNGVDYDASLGKSRIDTNQCIDSKNIDIYTP
                     EQIIAMGWQNKICSGDPANIHMSRTFLARMRLYVKIREMPPHDYQSTLSDYIVVQFDG
                     AGSVNEDPTAQNLKYHITGLENIRVLDCSVNFSISPETQVIDFGKFNLLDIRRHTMSK
                     TFSIKTTKSQNDQCTDGFKVSSSFYTEETLVEEDKALLIGNGLKLRLLDENASPYTFN
                     KYAEYADFTSDMLVYEKTYTAELSSIPGTPIEAGPFDTVVLFKINYN"
     gene            1126..2172
                     /gene="abrB"
                     /locus_tag="ACIYSB_004600"
     CDS             1126..2172
                     /gene="abrB"
                     /locus_tag="ACIYSB_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGC5431494.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKQSA"
     gene            complement(2169..2960)
                     /gene="nei"
                     /locus_tag="ACIYSB_004601"
     CDS             complement(2169..2960)
                     /gene="nei"
                     /locus_tag="ACIYSB_004601"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HGC5431495.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2996..3730)
                     /gene="pxpA"
                     /locus_tag="ACIYSB_004602"
     CDS             complement(2996..3730)
                     /gene="pxpA"
                     /locus_tag="ACIYSB_004602"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGC5431496.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            complement(3720..4652)
                     /gene="pxpC"
                     /locus_tag="ACIYSB_004603"
     CDS             complement(3720..4652)
                     /gene="pxpC"
                     /locus_tag="ACIYSB_004603"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGC5431497.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDSM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(4646..5302)
                     /gene="pxpB"
                     /locus_tag="ACIYSB_004604"
     CDS             complement(4646..5302)
                     /gene="pxpB"
                     /locus_tag="ACIYSB_004604"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGC5431498.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(5325..6068)
                     /gene="ybgI"
                     /locus_tag="ACIYSB_004605"
     CDS             complement(5325..6068)
                     /gene="ybgI"
                     /locus_tag="ACIYSB_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGC5431499.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            6339..7820
                     /gene="dtpD"
                     /locus_tag="ACIYSB_004606"
     CDS             6339..7820
                     /gene="dtpD"
                     /locus_tag="ACIYSB_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGC5431500.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITILFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7970..9388)
                     /gene="phrB"
                     /locus_tag="ACIYSB_004607"
     CDS             complement(7970..9388)
                     /gene="phrB"
                     /locus_tag="ACIYSB_004607"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGC5431501.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLNDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDHEGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(9385..9894)
                     /gene="ybgA"
                     /locus_tag="ACIYSB_004608"
     CDS             complement(9385..9894)
                     /gene="ybgA"
                     /locus_tag="ACIYSB_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGC5431502.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(10045..10629)
                     /locus_tag="ACIYSB_004609"
                     /pseudo
     CDS             complement(10045..10629)
                     /locus_tag="ACIYSB_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2439 a         2899 c         2616 g         2675 t
ORIGIN      
        1 catctggtgg cgcagtagaa aaatcagaag cgattcaacc gtttgctgta ccaggcaatg
       61 ccaaacccgg tgataagatc tgggaatctg acgatattaa aattcccgtc tattgtgaca
      121 acaataccaa cggcaacttt gaaagcgagc atgtctacgc ctgggtaaac ccctatccgg
      181 gggtgcagga tcgttattat caactcggcg tgacatacaa cggtgtcgat tacgatgcca
      241 gtctgggaaa aagtcgcatc gacactaacc agtgtatcga cagtaagaac attgatattt
      301 atacccctga gcagatcatc gcgatgggat ggcagaacaa aatttgctcg ggcgatcccg
      361 ctaatattca tatgtcgcgt acctttctcg ctcgcatgcg gttatacgtc aaaatacgag
      421 aaatgccgcc gcatgattat caaagtacgc ttagcgacta tatcgtcgtg caatttgacg
      481 gtgccggtag cgttaacgaa gaccctactg cccaaaacct gaaatatcat attactggtc
      541 tggaaaacat tcgcgtgctg gattgcagcg tcaatttttc catttcaccg gaaacacagg
      601 tgattgattt tggtaaattt aatttgctgg atatacgtcg ccacacaatg tcgaaaacgt
      661 tcagtattaa aacgaccaag agccaaaacg atcaatgcac cgacggattt aaagtcagtt
      721 cctcctttta taccgaagaa acgttggttg aagaagataa agcattgttg attggtaatg
      781 gtctgaagct acgtttactg gatgaaaacg cctcgcctta taccttcaat aaatatgctg
      841 aatacgccga tttcaccagt gacatgttgg tctacgaaaa aacctatacg gctgaacttt
      901 cgtccattcc aggcaccccc atcgaggctg gccccttcga tacagtggtg ctttttaaga
      961 ttaactataa ctgatttacc atgatgccct ttgccgccgg aaagggcatc tctttcagaa
     1021 aattgctgat tttccccttt tccttgattg ctgttatttt ttcctggccc agctttcttt
     1081 atgctgtatc gatacaccaa agagaaaata atgagggagc gaaggatgcc agttttgcag
     1141 tggggaatgt tatgtgtgct atcacttctc ctttctattg gtttcctcgc ggtacatctc
     1201 ccggcggcgc tattacttgg gccgatgatc gccgggatca tctttagtat gcgcggaatt
     1261 actctgcaac ttccccgctc tgcttttctc gccgcccagg ccattcttgg ctgcatgatt
     1321 gcgcaaaacc tcactggttc gattctcacc accctggccg tcaactggcc gatcgtgctt
     1381 gcaattttac tggtaacgct gctttccagc gccattgtgg gttggttatt ggtgcgctat
     1441 agctcattgc ctggaaatac cggtgcctgg ggctcctctc ctggcggcgc ggcggcaatg
     1501 gtcgctatgg cgcaagatta cggcgcagat attcgcctgg tggcgtttat gcaatatctg
     1561 cgagtgctgt tcgtcgcggg ggccgctgtt ctggtcaccc gcatgatgct gggcgataac
     1621 gctgaagcgg ttaaccagca tatcgtctgg ttcccgccag tgagcattaa tctcctgctt
     1681 actattttgc tcgcggtcgt tgccggtaca gtgggatgcc tgctgcgtct tccttccggc
     1741 acgatgctca tcccgatgct ggcgggcgca gtgctccagt ctggtcagct catcaccatc
     1801 gaactaccgg aatggctgct ggcgatggcg tatatggcaa ttggctggcg gattggtctt
     1861 ggtttcgata aacaaatctt actgcgggca ctacgcccgc taccgcaaat cctgctgtcg
     1921 atttttgctc tgctggctat ttgtgcgggt atggcgtggg ggctgacccg gtttatgcac
     1981 attgatttta tgactgccta cctcgccacc agccccggcg ggcttgatac cgtggcggtc
     2041 atcgccgcag ggagcaatgc cgatatggcg ctcattatgg cgatgcaaac cctacgcctg
     2101 ttcagtattt tgctgacagg gcctgccatt gcgcggttta tttcaaccta tgcgccgaag
     2161 cagtcggcct agtgctggca gccagggcac cagtaaaacg ggcgagatga cagcgtggtt
     2221 ttctcaatga tgctgccaca acgttcgcac ggttcgccat ctcgatgaaa aaccttaaag
     2281 cgaaacagcg ccccatgatg cttattctca tccacctgcc cccgcgtagc gtaggaaaat
     2341 cgaggaatct ccagtaacgc gtgtgcgagt gcatccagtt gcgccgcatt gagatctttc
     2401 gctttatgat ttccagtcaa cccaacctgc cagaggatct ccacccgcaa ataattgcca
     2461 agcccagcca gaaacgcctg atcgagcagt aatccagcaa actgacggtt acgaaagcgc
     2521 ggcgacaata atcgttcttt caccacctcc ggcgtcagat tcggatccag cacatcggga
     2581 ccaacgcgtt gtaaaaacgg atgcgtggtc agttgttccg gggtcaacat ctcaatatcc
     2641 gaggcgctat aaagcagaat ggttttgtca gccgtttgca gttttacccg caatactcgc
     2701 gtggtctgcg gctcttcgcc ggtatcaacc acgcgccaga cgccgtaaag ctgattatgg
     2761 ctgtagagcg ttaagtcgtt ggaaaaatga gttaacaacg ccttaccacg cgtttccaca
     2821 tgggtaacgt gttgaccgat aagttgtgat tgataaggtt ttaactgcgg gaaggcaaac
     2881 cagacatcag ttagtggttt gcctttgatc gccgcctcca ggttatccgc tgcacggcgg
     2941 atctccgggc cttcaggcat ggtgatatcc ttttttcatt ctttattaat cggggttatg
     3001 ctgcgacaac aatccccttt tcggcaaatg cagagcgtag tcggcgggcg aacgccagtg
     3061 cgtgctcgcc gtcgccatgc aggcagaccg tttgcgccgc gaccgttgcc cattcgccgg
     3121 tgatactttt gactctgccg tgttgcacca tctccagcgt ttgcgccagc gcctgttctt
     3181 cgttttcaat caacgcgcct gactggcttc gcggcaccag cgagccgtca gcctgataac
     3241 cgcgatcggc aaacacttcc tcgcgcgttg tcagaccata ttgcttgcct gcacgaatca
     3301 gctcgcttcc cgccagcccg acgagaatca atgctggatc gcaagcgtat accgctctgg
     3361 cgatggcgtc tgccagttgt gcttctttcg ccgcctggtt gtacaacatg ccgtgcggtt
     3421 tgacatgacg cattacgccg ccttgcgcac gggcaatcgt tgccagcgcg ccaatttgat
     3481 acagcgtctg ggcgtaaacg gtttctggcg gcagctgcat ggcgctgcga ccaaaatttt
     3541 ccctgtcggg aaaactcggg tgagcgccaa tcgcgacacc attttttatt gcttcacgca
     3601 cgcaagcctg catgatttgc gcatcgcctg catgaaatcc acaggcaata ttggcagagg
     3661 aaaccagcgt taatagctct gcgtcgctgg cgcagccttc gcccagatcg gcgttcaggt
     3721 caattttcat tgtgcagccg ccacgctaat tgttcaaaat aacgttgctg atcttgccgt
     3781 gcttttagtg cctcttccag tgaacactgg acaaaatgaa tcggctgacc gagcggaatt
     3841 tgcgccagat ggtacatatc agcctcaatg atacaggcaa tacgcgggta accaccggtg
     3901 gtctgtgcgt cgttcatcaa cacaatcggc tgcccgttat gtggcacctg caccacgccc
     3961 ggtaacaaac cgtgagataa cagttcgcga tcggtggtgc gttttaaaat ttgcccctgt
     4021 aagcgatagc ccatgcggtt actttgcgag ctaagctgcc agggcgaacg ccagaatgca
     4081 tcctgcgagg cgcgatcgaa ctcatgatat tccggccccg gcaaggcgcg aatgcggttg
     4141 ccccacagca gctgtttaac gccctgcgct tccatagaat cacgcttcga tttgccaatc
     4201 gggagtcggt caccatcctt cagtaaacgg ccttccagcc cgccaatccc cactttgaga
     4261 tcggtgctgc atgagcccat taccggcgga acatcaatac cacccgcgac cgccagataa
     4321 ctgcgcatcc cgtgctgcgg gcgtttaagc gttaaacgct ggcctgcttt catcggcaat
     4381 cgccagccgg tccagacggc attatcatcc agccgcgctt cgcaaccggc acccgtcaga
     4441 gcaaaccacc catcagtttc gaactcaaca gttaactgac cgagcgtgat ctccagcgcg
     4501 ggggcattgg cgtcattacc caccagtagg ttagcaatgc gtaacgcggg catatccagt
     4561 gcgccgcagt ggctgatacc cgactggcga aaaccgtgac gaccgccatc ctgcacagtg
     4621 gtatacatgc ccgcacgaat aatcttcagc atactccctc cttctgcggt acaaagcgca
     4681 cgctgtctcc cggacgtaat aagatgggtt cgtcacgcgc cggatcaaac aggctgagtg
     4741 aggtatgacc aatcaactgc cagccacccg gcgttgccag cggataaaca ccagtctgcg
     4801 gcccgccgat cccgacagaa cctgccggaa cgagtaagcg cggttcagcg cgccgtggcg
     4861 tgtgtagttg ttccggcaaa ctcccgagat acgggaagcc cggttgaaaa cctaaaaacc
     4921 agaccacgta ttccacggag gagtgcaatt caacaacctg tttttcgctc aacccgcaat
     4981 gcgccgcgac caccgccaaa tccggtccgc ctgcaccacc gtaaaccacc ggaatttcaa
     5041 taaagcgaga ctccggctcc agcgcctcgc tctcctccca ccagcgttgc aaacgctcta
     5101 tggcatccag cgccagcgac tcaggattac gcaaaatcac cgtgatattg ttcatgccgg
     5161 gaatggcttc aaccacattc ggcatatcca ccagacgctg cgccagtcgc cagatccgtt
     5221 tctggctagc cagcgtcacc ggcggttcca gttccagcac taccgccgtt tcacctatca
     5281 gataacaacg cgctcgttgc actttcgatc cctctgatta ttcgttatgc aggattagga
     5341 atatcaataa aggtcacatc aagatcggta ttttcattca gccactcgct caatgcgcga
     5401 ataccaccac gttcagtggc atggtgacct gcagcataaa aatgcaatcc ctgctcgcgg
     5461 gctgaatgaa tggtctgttc agaaacttcg ccagtaataa aagcatccac gccaaaacgc
     5521 gcggcgctat cgataaaact ttgcccgccg cccgtgcacc aggcgacgcg ctggaccacc
     5581 tcaggtccgg tatcgccaca ccataacggc ttacgtccca gacgcgcttc aatccaggaa
     5641 gccagctcca gtcccggcac aggcatggtc agttcgcccc acggcaccaa cggctcaatt
     5701 tcgcccatga ccgtgatccc cagtaacgcc gccagctgtg cgttattgcc cagctcagga
     5761 tgtgcgtcaa gcggcaaatg ccagccatac aggttgatat cattcgccag caacgttttt
     5821 aaacggttac gcttcattcc gcgaatgacc ggagactctc ctttccagaa gtagccatga
     5881 tgcacaatga ccgcatccgc gcccagacgc actgcctcat cgagcaaagc ctggctggcg
     5941 gtgacaccgg taacaatttt ttgcaccgtc tctttgcctt ccacctgcaa accattcggc
     6001 gcgtaatcac taatcgccgc gctgttcagt ttttcgttaa tcagttgttc cagttcggtg
     6061 tttttcatct tcgcgccttc cgtattctga tagctacacc gttaacatag cgttacctcg
     6121 ccagttcgtc gatagccaat cttttgacat attaagaaac aaacccgacg ccaaaagacg
     6181 caaattgttt atatcatcac gcttcagcta atgtatattt atgcaatcag acagcatatt
     6241 gcataaataa ttagatctgt atcaaagttt taactttaag gaaacacaag cacgccgcgc
     6301 aactattacg tgacgttgcc gtcaattgtg gaattattat gaataaacac gcatcacagc
     6361 cgcgcgctat ttactatgtc gttgcgctgc aaatctggga atattttagc ttttacggca
     6421 tgcgtgccct gctgattctc tatctcacca atcaactaaa atacaacgat actcacgcct
     6481 acgagttatt tagcgcctac tgttcgctgg tgtatgtcac gccaatcctc ggtggctttt
     6541 tggcggataa agttctcggc aatcgcatgg cggtgatgct gggggcgttg ttgatggcga
     6601 tcggtcatgt ggtgctgggt gccagtgaga tccatccgtc attcctctat ctgtccctgg
     6661 cgattatcgt ctgcggctat ggcctgttta aatctaacgt aagctgtctg ctcggcgagc
     6721 tgtatgagcc aaccgatccg cgtcgtgatg gcggtttctc gctgatgtat gcggcgggta
     6781 acgtggggtc tattatcgca cctatcgcct gtggttacgc ccaggaagag tacagctggg
     6841 cgatgggctt tggcctggcg gcggttggca tgatcgcggg tctggtcatt ttcttatgtg
     6901 gcaatcgtca tttcactcat acccgcggcg ttaacaaaaa agtactgcgt gcgacaaact
     6961 ttctcctgcc gaactgggga tggctgctgg ttctgctggt ggcaacgcct gcactgatta
     7021 ccatactgtt ctggaaagag tggtcggtat acgccttaat tgtcgcgact attattggcc
     7081 tgggtgtact ggcaaaaatt tatcgcaaag cagaaaacca gaaacagcgg aaggagctgg
     7141 ggctgattgt gacgctcacc ttcttcagta tgttgttctg ggccttcgca caacagggcg
     7201 gtagctcgat tagcctttat atcgaccgct tcgttaaccg cgatatgttt ggttataccg
     7261 ttccgaccgc gatgttccag tcgattaatg ccttcgcagt tatgctgtgc ggtgtgttcc
     7321 tggcgtgggt ggtaaaagag agtgtcgcgg gtaatcgtac cgtgcgcatc tgggggaaat
     7381 ttgctcttgg ccttggcctg atgagcgccg gattctgcat tctgacctta agcgcccgct
     7441 ggtccgcaat gtatggtcac tcttctctgc cactgatggt attaggcctg gcggtgatgg
     7501 gctttgcgga actgtttatc gacccggttg ccatgtcgca aattacgcgc attgaaatcc
     7561 ccggtgtgac cggcgtatta accggcatct acatgctgct ttctggcgcg atcgcgaact
     7621 atctggcggg cgtgattgcc gatcagacat cgcaggcttc gtttgatgct tccggggcga
     7681 tcaactactc catcaatgca tatattgaag tatttgatca aattacctgg ggcgcactgg
     7741 cgtgtgtagg actggtactg atgatttggc tgtatcaggc gctgaaattc agaaaccgcg
     7801 cgctggcgct ggagtcttaa taggccagca ccgatgcctg atgcgacgct tgacgcgtct
     7861 tatcaggcct acgtatttcc tgcaatttat tgaatttgca caaatttgta ggctggataa
     7921 ggcgttcacg ccgcatccgg catctgggct cgattgcctg acgcgtctgt tatttcccct
     7981 tccgcgccgc ctcatacgct gccaacgttt gtactctcgc ttctttgtgc tcgactatcg
     8041 gttgcggata atccagcgtc acacctgctt tctgcgccca cttccacggc tcatgcacca
     8101 ctttccctgg cacatcgcgc agttccggta gccactggcg gataaactcg ccctcatgat
     8161 caaatttctc gccctgggtt gtcgggttga aaatacgaaa atacggcgct gcatcggttc
     8221 cggttgaagc ggcccactgc cagccaccgt tattggctgc caaatcacca tcaatcagct
     8281 gcgacatgaa atatcgctcg ccttcgcgcc agtcgttcaa taaatctttc accagaaaac
     8341 tggctgtaat catccgtagc ctgttatgca tccagccagt gctgttaagc tgacgcatag
     8401 cggcatcaac aatcgggtat cccgttttgc cttcctgcca ggcctgtaaa tgtgcgggat
     8461 tgctctgcca ctgtacacga tccgtccagg caataaatgg acgatgttta cacaacgagg
     8521 ggtgatacgt tatcaggtga cggtaaaact cgcgccagat cagctcatta agccagacac
     8581 taccggcccc accgtccagc gcctgcggct gttcagccaa caagcgatgc aagcactggc
     8641 gaggcgataa cccgcccgtt gccaggctgg ccgacaaacg gctggtgcct tccactgccg
     8701 gaaaatctcg ttgttgctca tattctccgg caccgttctg gcaaaactgg cgtaattgcg
     8761 caatcgccgc tttttcttcc accggaaaat gcgcagtatc gaaagactga cgaggataat
     8821 tcagcgtaat ggatggcgag ggctctatcg atccgctact acgaactttt ggcgcagcga
     8881 cgcactccgg catcccttcc cgcagccgtt tcagccaggc attcttaaaa ggcgtaaaga
     8941 ctttgtacat ctcgtgatta ccggtcatca ccgcgccagg cggcaggatc acgctgtcat
     9001 caaatccttc acacaccacg ttacgcagcg ctctttcaac ttccacatcc cgcgcccgct
     9061 cattcacttc atactgatag ttataaaaca ggtgggtaac gctgttttcc gcgcacacct
     9121 gtttaactat ttcgacactg gcgacaaagt catccacttc acggaacaat aaaggaatac
     9181 ctttttccgc aagcgctatt tgtagcccat tcagttgagc attgataagt tcagcctgac
     9241 gcggcgacat gttatgcgtc gcccactggc gtggtgtagc gatatacaac gccagcacgc
     9301 gtgcagacga attgcggcag gcggcagcca gtgcgagatt atcgtgcaga cgtaaatcct
     9361 ggcgaaacca gaccagatgg gtagtcataa aactcctgaa tggcgcaagt taatcacacg
     9421 cggccaaagt tcgaaataac gctgccctga aagccaggcg tcaggataaa gcgccatata
     9481 gtgtttgata cgcatcagcg gcgcaagaag tggttgctcg ccacggcgat aactgtcgat
     9541 aagcgcagcc agctgctggc gttctgtgga atcaatatgc gggcgaaaat aaccctgaac
     9601 gtgcatcaaa acattggtgt gatcgcgcgg gttggcgggg tgagaaagca aaacaactac
     9661 gcgttggcgg tactggttgt aatagtcatc cagattttgc cactggtgaa tatcagccac
     9721 gaacgggcca agtttgcggt actccggctg agaatgcgcc agaaagacga gtttatagcg
     9781 gctgtgataa tcgagaagcg ccccgcgcgt taagccatgt tctttcagtc gatgtaactc
     9841 atgaagggca aagatacgga ttagggtgct gtcatcaaat cgttgtagat tcattactgc
     9901 tcctgagcgg tttacaggag taagtgtaga cgttgagaaa tgatatttgt gaagttaatc
     9961 ctgttgtaac aggacaaatt tacgaaagcc tgctccccgc aaggactgac gtcaggtagt
    10021 ttctaaccat agctgccctt tctattcaaa cgtgaaatca ataagctcat cagggacatc
    10081 gcaaacaata tgaagacggg tttcttctct gccgtgactc ttttcactaa ttgcgtaact
    10141 gtcatgcttg ggattattta attctttcag cggaaatttt tcctcgaagg ctttatttag
    10201 ccgcccctgg tttcctttta cagcgaataa ataatcacct ccctgttttt gtatcttctc
    10261 tgcaatatct ttctggcaac ccatcgcatc ggttttgatg atttttcctt taatatccag
    10321 catgttaaga agttcaggga tagctgtgat ctcattggat ttcttatccg tcttgatctg
    10381 tccgatgacc agactgtgca ttgttgagaa cgcactaatg acatgaatcg ctcccttacg
    10441 gcgactcttg tcataagaat gccggtgtat ttttccatca attgcgatga cgtctttatc
    10501 atctgaagaa tggtagtcaa gcatccagtt aataaagctc tcatgaaatt tcgcaggaca
    10561 gatacaggat acaactctgg caatggtatc gtgaacagga ataccatttt caaaatcacc
    10621 atattgctt
//
LOCUS       DBBGLQ010000132        10584 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.132, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000132 DBBGLQ010000000
VERSION     DBBGLQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.132"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(430..2844)
                     /gene="ybbP"
                     /locus_tag="ACIYSB_004610"
     CDS             complement(430..2844)
                     /gene="ybbP"
                     /locus_tag="ACIYSB_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGC5431503.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(2841..3527)
                     /gene="ybbA"
                     /locus_tag="ACIYSB_004611"
     CDS             complement(2841..3527)
                     /gene="ybbA"
                     /locus_tag="ACIYSB_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGC5431504.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            3498..4121
                     /gene="tesA"
                     /locus_tag="ACIYSB_004612"
     CDS             3498..4121
                     /gene="tesA"
                     /locus_tag="ACIYSB_004612"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGC5431505.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            4111..4920
                     /gene="ybbO"
                     /locus_tag="ACIYSB_004613"
     CDS             4111..4920
                     /gene="ybbO"
                     /locus_tag="ACIYSB_004613"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGC5431506.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            4981..5835
                     /gene="cnoX"
                     /locus_tag="ACIYSB_004614"
     CDS             4981..5835
                     /gene="cnoX"
                     /locus_tag="ACIYSB_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGC5431507.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQESNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5898..6677)
                     /gene="fetB"
                     /locus_tag="ACIYSB_004615"
     CDS             complement(5898..6677)
                     /gene="fetB"
                     /locus_tag="ACIYSB_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGC5431508.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(6664..7341)
                     /gene="fetA"
                     /locus_tag="ACIYSB_004616"
     CDS             complement(6664..7341)
                     /gene="fetA"
                     /locus_tag="ACIYSB_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGC5431509.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            7487..8404
                     /gene="qmcA"
                     /locus_tag="ACIYSB_004617"
     CDS             7487..8404
                     /gene="qmcA"
                     /locus_tag="ACIYSB_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGC5431510.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            8401..8859
                     /gene="ybbJ"
                     /locus_tag="ACIYSB_004618"
     CDS             8401..8859
                     /gene="ybbJ"
                     /locus_tag="ACIYSB_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGC5431511.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(8860..9267)
                     /gene="cueR"
                     /locus_tag="ACIYSB_004619"
     CDS             complement(8860..9267)
                     /gene="cueR"
                     /locus_tag="ACIYSB_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGC5431512.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(9264..9361)
                     /locus_tag="ACIYSB_004620"
                     /pseudo
     CDS             complement(9264..9361)
                     /locus_tag="ACIYSB_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9392..>10584)
                     /gene="ybaT"
                     /locus_tag="ACIYSB_004621"
     CDS             complement(9392..>10584)
                     /gene="ybaT"
                     /locus_tag="ACIYSB_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGC5431513.1"
                     /translation="LGQAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGII
                     DFFRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYAL
                     GIIAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGA
                     FFSCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDV
                     SALELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSE
                     RELPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAI
                     RLRHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKR
                     NKTV"
BASE COUNT         2621 a         2920 c         2678 g         2365 t
ORIGIN      
        1 taaagtgccc ttttagttat tgttcatatc cctgtgttag caacaaaaaa aaataaaatg
       61 acacacaaga ccagctaatg tatttatata tttttcacat aaacttattt gttctcaatc
      121 aacaacaact gttattacag gtcactttca atattttatt aaactaacat ttttagcaca
      181 gatgaattca tcatcaacaa attcactata tggataatat ccattcaacg catatgattt
      241 atgattttat tagtctgagt tatttttgta gggctatatt taaaatcaat aaactccacc
      301 gattcagatt aatatcagca atagaaagat aggaaatcac caattcaata acatcatcaa
      361 tgaaacatat tgtagaatta atttaaacaa ggcgttccat tcaaccagaa cgccccttat
      421 ttactctcat caccccgcaa actgcctgaa cagcgcctta cccttaacca gtctcgcgcc
      481 cagccagccg ccgaaaagcg acagcagcag cgcaccgctg cacggcagaa caatccacaa
      541 tcgccagtct ggctcccacg ggaaatcaaa cactttcgcc tgcaacactg ccagtgccgt
      601 ttccgcacca attgcggcca ccaggccgga aacaaaccca agcatggcga actcacacca
      661 caatgtcgtg cgcagcagtt ttttccccgc accgagtgtg cgccatacca ccagctcctg
      721 atgacgctgg cgcattccca cctgcacctg tgccagcaac agcaacatac cgcaggcggt
      781 gaccagcacc accatcacct ccagcgcccg acttacctgc tccagcacct gaccgacctg
      841 ttttaaaatc gcgccaatat ctaacaggct aatggtcggg aactggcggt tgagttgcgt
      901 caacatgccg ttgccattct cccagcggaa actggtaagc cagctctgcg gttgcccgtc
      961 taatgcccct tccgggaaaa taaaatagaa attaggccgc agactttccc agtccacttt
     1021 gcgcaggctg gtcactttag cgcggaactc ctgggtatcg cccataaaag tcacggtatc
     1081 accgagggca acgtttaagc gtttcgccag cccctcttcc atcgacactt catcggcttt
     1141 tggcggccag ttaccggcga caatcggatt atgatcgggc cgcgtatttt gccaggtaag
     1201 attgagttcg cgattaagcg cctcatcttc attaccttct gtcggcttat cgttaatcgc
     1261 cgtcagccgc gcccgcacta ccggataaaa tgattccggg actatatgat gttccgcgag
     1321 gaacgctttt agcggtgcta cctgttctgt ggcgatgtta attaaaaagt agttcgggct
     1381 ttctggcggt agctgctgct gccagcggtc gagcagatcg ccacgcaata ccagcaacag
     1441 cgccagcagc ataaaggaga gcgaaaatgc cgaaagctga cttaacgttg accacggctg
     1501 acgtaacagg cggctaaccg ccaggcgcag aggcagcgat ttcagcgtca tgcggcgaag
     1561 tacattcagc agcatccagc ccagcacacc gcacagcaaa gccagtacta ccgcgcccgc
     1621 cagcaccgcc caaagcagca tgctgccacc cattaatccg gcgagcagca gcacaaccac
     1681 cacactgaca atcggcagat aaaacttcag cggccagacg ttcgctacca catcattacg
     1741 taatacgcgt aaaggctgcg ttgccagcaa caagcgatat ggtcgtagcc ccaccagcag
     1801 cgagatgacc gtcatggtgc caagcgccca cagccacggc cagaggctgg ctggcggtag
     1861 tgcagcaggc agaacgggct tgagcagcac catcaacacg ttttcgaaca acagacctat
     1921 ggccccaccg gtaacggctg aaagcgtcag caccatcaac cactgaccga cgattagctt
     1981 acgcagttgc gctcgccctg cccccagcgt tttgaggatc gccaccagat cgtagcgact
     2041 gcgacagtaa tgattcatcg ctaccgccac cgctgccact gccagcagca aggtcagaag
     2101 cgccgaaagc agcaggaact gttgcgagcg ttccatcgat cgccccagcg cgccttcgtc
     2161 ctgttccaga ccgtaccagc gttgttcggg tttaagctga ggtaacaacc atttctcata
     2221 gccgtcgagc tggttctcgt tgccgccgaa tttataacgc caggtgaccc gactccccgg
     2281 ctgcacggct ccggttttat cgacatccgc cagattcatc atcagacgcg gagccatctg
     2341 gaaggggtta aaaccggaat ccggttcctg aatcacttct ccggcaatcc gcaaggtggc
     2401 atcgcccacg tcaatggtgt cgtccgtttt caggttaagc agtgccatca ggcgtggggc
     2461 cagcaatacg ctgcccgcct gcggtttcag gccaggggga ttagtttgca gatcgccata
     2521 catcgggtag atatcatcca ccgctttgac gttcgccagc tgcggtgtgt cgcctgcaaa
     2581 ggtcattgtg gcgaaagtca gctgcttgcc gactttcagg ccgcgctttt gcgcttcctc
     2641 cagccacgct tgcggcactt cgcgtgaact gcgcaacgcc cgatcgcccg ccataaactc
     2701 acggctttgc tggcttaagc ccttctccat gcgatcgctg atattgccca gcgccagcac
     2761 gcaggccacc gccaggctta gcgccagcca gacaattaat agcgacggcg aacgccattc
     2821 gcgccagaac caacgtgcaa tcatgcttcc tcctgcaact gcccgttcac cagccgtaag
     2881 cagcggtcgc agcgtgccgc cagttgcagg tcgtgggtca ccataatcaa cgtggtgcca
     2941 tgttcacggt tgagggaaaa cagcaggtcg gcaattttat cgcccgtctg gcggtcaagg
     3001 ttgccggtgg gttcgtcggc aaacagcaca tcaggtcgac cattaaaggc tcgtgccagc
     3061 gccactcgtt gctgttcacc gccggaaagc tgtgccggaa gatgatccag acgtttaccc
     3121 agccctaact gttcgagcaa cgctttcgcc ccgttacgac tttccgcgct actctcaccg
     3181 cgcagcagag ccggaagctc gacgttttcc agcgcgttaa gggtaggaat taacataaat
     3241 gactgaaaaa caaagccgac gtgcttcgcg cgcaactttg cccgcgcttc ttcgtccata
     3301 ttatgtagcg gttgtcccac cagactcact tcgccactgc tgccgtcatc aagcccggcg
     3361 aggatcgcca gcaaggttga cttacccgat cccgactcgc ccaccagtgc gatggtctcg
     3421 ccacgtttga caaccagctc aactccggtg aggatggaga gttcatgctc cccctgaccg
     3481 acggacttct taagatgatg aacttcaaca atgttttccg ctggcatttg cccttcctgt
     3541 tcctggtcct gttaaccttc cgtgccgccg cagcggacac gttattgatt ctgggtgata
     3601 gcctgagcgc cgggtatcga atgtctgcca gcgcggcctg gcctgccttg ttgaatgata
     3661 agtggcagag taaaacgtcg gtagttaatg ccagcatcag cggcgacacc tcgcaacaag
     3721 gactggcgcg ccttccggct ctgctgaaac agcatcagcc gcgttgggtg ctggttgaac
     3781 tgggcggcaa tgacggtttg cgtggttttc agccacagca aaccgagcaa acgctgcgcc
     3841 agattttgca ggatgtcaaa gccgccaacg ctgaaccatt gttaatgcaa atacgtctgc
     3901 ctgcaaacta tggtcgccgt tataatgaag cctttagcgc catttacccc aaactcgcca
     3961 aagagtttga tgttccgctg ctgccctttt ttatggaaga ggtctacctc aagccacaat
     4021 ggatgcagga tgacggtatt catcccaacc gcgacgccca gccgtttatt gccgactgga
     4081 tggcgaagca gttgcagcct ttagtaaatc atgactcata aagcaacgga gatcctgaca
     4141 ggtaaagtta tgcaaaaatc ggtcttaatt accggatgtt ccagtggaat tggcctggaa
     4201 agcgcgctcg aattaaaacg ccagggtttt catgtgctgg caggttgccg gaaaccggat
     4261 gatgttgagc gcatgaacag catgggattt accggcgtgt tgatcgatct ggattcacca
     4321 gaaagtgttg atcgcgcagc cgacgaggtg atcgccctga ccgataattg tctgtatggg
     4381 atctttaaca atgccggatt cggcatgtat ggcccccttt ccaccatcag ccgtgcgcag
     4441 atggaacagc agttttccgc caactttttc ggcgcacacc agctcaccat gcgcctgtta
     4501 cccgcgatgt taccgcacgg tgaagggcgt attgtgatga catcatcggt gatgggatta
     4561 atctccacgc cgggtcgtgg cgcttacgcg gccagtaaat atgcgctgga ggcgtggtca
     4621 gacgcactgc gcatggagct gcgccacagc ggaattaaag tcagcctgat cgaacccggt
     4681 cccattcgta ctcgcttcac cgacaacgtc aaccagacgc aaagtgataa accagtcgaa
     4741 aatcccggca tcgccgcccg ctttacgttg ggaccggaag cggtggtgga caaagtacgc
     4801 catgctttta ttagcgagaa gccgaagatg cgctatccgg tgacgctggt gacctgggcg
     4861 gtaatggtgc ttaagcgcct gctgccgggg cgcgtgatgg acaaaatatt gcaggggtga
     4921 gttgaagcgc gcgcttaagc ccccatgtca aagaaaatat cgacaacaga gagcgactcc
     4981 atgtccgtag aaaatattgt caacattaac gaatctaacc tgcaacaggt tcttgaacag
     5041 tcgatgacca ctccggtgct gttctatttt tggtctgaac gtagccagca ctgtttgcag
     5101 ttaaccccaa ttctggaaag cctcgcggcg cagtacaacg ggcaatttat tctggcgaag
     5161 ctggactgcg acgcggagca gatgattgcc gcgcagtttg gtctgcgtgc gattccgacc
     5221 gtgtatctgt tccagaacgg gcaaccggta gatggcttcc aggggccgca accggaagag
     5281 gcgatccgcg ccctgctgga taaagtgctg ccgcgcgaag aagagctgaa agcgcagcag
     5341 gcgatgcaac tgatgcagga aagcaattac accgatgccc tgccattgct gaaagacgcc
     5401 tggcagttgt cgaatcagaa cggggagatc ggcctgctgc tggcagaaac gctgattgcg
     5461 ctgaaccgtt ctgaagatgc ggaagcggtg ctgaaaacca ttccgttgca ggatcaggac
     5521 acccgctacc aggggctggt ggcgcaaatc gaactgctga agcaggcggc tgatacgccg
     5581 gaaattcaac agttgcaaca gcaggtggcg gagaatccag aagatgccgc actggcgacg
     5641 caactggcgc tgcaactgca tcaggttggg cgcaatgaag aggcgctgga gttgctgttc
     5701 gggcatctgc gtaaagatct caccgccgca gacggtcaga cgcgtaaaac gttccaggag
     5761 atcctcgctg cgctgggtac gggtgatgca ctggcgtcga agtatcgccg ccagctgtat
     5821 gcattgttgt attgatattc cgttgatcgg cacatgaacc acaacgccgg atgcgatgct
     5881 gcgcatccgg catcgcatca tttcttcttc aattgcgtca ccaccaactg gtggcgcgaa
     5941 ttataaaact tacgataggt taaatagcag gcaataatgg tcgacaagct ggcggttgag
     6001 agcagcataa aggtcaccat aatctgatat ttaatcgcct tcaccggatc aatcccggca
     6061 aatatcagcc cggacatcat tcctggtaaa ctcactaagc caaccgtttt tgctgaatcg
     6121 accgtcggaa ttaaagccgc gcgaatactg tcgcgaatca atatcgctga agcctgcttc
     6181 ggcgtcgcac caagactcag tttctcctga atctgttgct gttcgctaat gacccgttgc
     6241 cctaaattgt tgtaacacaa ccccaccgct accatggcgt taccggcaat catcccggcg
     6301 atagggatca cctgcatcgg gataaattca atcgaccctg agagaatcag caccgccagg
     6361 gtaattcccg ccccgaccgt aatggcaata aacgatgaga taaaagcttt agcaatatat
     6421 ttactgcgtt tttgcgcgtt ccacgccgca ttaaagcaga taaataacac catcaataat
     6481 gtcaggctgg catcatccac gctgaaaata tacttcagca catagccgac aataatcagc
     6541 tgaattatcg ctcgcccgac gctccagaga atatctttct ccagcgccag tttttcttta
     6601 tggctaatta agattgccac caccaccagc attaatgcca gtgctaatga ttcgttagta
     6661 atattatgcg agttcatagc gtgcttcctg catttctccg gcatgcggtt gcagtgtaat
     6721 cactttatcc gcatgattaa tttcgtcttt atcgtgtgtc acccacagca cggcaatatt
     6781 ttgctcgcgc acataacgat ggatcatctc attgacgtta tgtttattac tttcatccag
     6841 cgcactggtt atttcatcca gcaataaaac cttcggcata aattgcaggt tacgaatcaa
     6901 tgagatgcgt tgtttttcac caccagatag ctcggcgata ttcttcgtca aaatgctgtc
     6961 cggcaaggcg aagcgttcga gaaaatcgag aaaaatggct gggtcaggct gccggttacg
     7021 gatctgccag ggaaagatca gattatcgta taccgtatcg ccaaacagcg tcggtgtctg
     7081 ggcgcagtaa gagacttgtt ggcggtagat ttctggcttt agtgtgctga catcctcacc
     7141 ttcaaacagt aacgttccgc tggttgggct gatcaatgaa gcaactattt ttagcagcgt
     7201 acttttgcca caaccagaag gaccggtaat taacttaaat tcgccagcac gcagcgaaaa
     7261 attgatgtta ttaagaatct tcgcatcacc cgccagatat cctacgtttt gtagctgaag
     7321 caaaggacta ttttcctgca tcgctgttcc ctttttctga tttttactaa aaacagttta
     7381 tccttcgcag gaataagggg gaactctctt tcagtaatca ggtataattt gtttaatcaa
     7441 gcagcaattt tgctgattat ggacggtaca acaggaggtt tttccgatgc ttatctttat
     7501 cccgattctc atttttgtcg cgctggtcat tgtcggcgcg ggtgtcaaaa tcgtaccgca
     7561 gggctatcag tggacagtag aacgctttgg tcgctatacc aaaacgttac agccggggct
     7621 cagtctggtg gtgccgttta tggatcgcat tggtcgcaag atcaatatga tggagcaagt
     7681 gctcgatatc ccttcccagg aagttatctc gaaagataac gccaacgtta ccatcgacgc
     7741 cgtgtgcttt attcaggtga ttgacgcgcc gcgcgcggct tatgaagtca gcaatctgga
     7801 gctggcgatc atcaacctga ccatgactaa catccgtacc gtgctgggtt caatggaact
     7861 tgacgaaatg ctctctcagc gcgacagcat caactcacgc ctgctgcgta ttgtcgatga
     7921 agccaccaac ccgtggggga ttaaagtcac ccgtattgaa attcgcgacg tgcgcccacc
     7981 ggcagagctt atctcttcaa tgaacgcgca gatgaaagcg gaacgtacca aacgcgctta
     8041 cattcttgaa gcggaaggga tccgtcaggc ggaaatcctc aaagccgaag gtgaaaaaca
     8101 gtcgcaaatc ctgaaagcgg aaggcgaacg tcagtcggcg tttttacagg ctgaagcgcg
     8161 tgaacgttcc gccgaagcag aagcccgcgc caccaaaatg gtgtctgaag ctatcgcctc
     8221 cggtgatatt caggcggtga actacttcgt agcgcagaaa tacaccgaag cgttacagca
     8281 gatcggttcc tccagtaaca gcaaagtagt gatgatgcca ttagaggcca gcagcctgat
     8341 ggggtcgatt gccgggattg ccgagctggt gaaagacagc gccaacaaac ggactcagcc
     8401 atgatggagt taatggtcgt tcatccacat attttctggc tcagtctcgg cggtttgctg
     8461 ctggcagccg agatgctggg cggaaatggt tatttgttgt ggagcggcgt ggcagcggtg
     8521 attaccggcc tggtggtctg gctggtgccg ctgggttggg agtggcaagg ggtaatgttt
     8581 gccatcctga cgctgctcgc cgcctggctg tggtggaaat ggttgtcgcg gcgggtgcgc
     8641 gaacaaaagc acagcgatag tcatttaaac cagcgcgggc agcagctgat tggccgacgt
     8701 tttgtgctgg aatctccgct ggtcaacggg cgcggtcata tgcgcgtcgg tgacagttca
     8761 tggcctgtca gcgccagcga ggatctcggt gcaggtacgc atgttgaagt cattgcgata
     8821 gaagggataa ccctacatat ccgagccgtc tcgtcttaat caccctgccc gatgatgaca
     8881 gcagccggag agattttcga taatcgggca gtcggcgctg tcatcgccag ggcaggcatt
     8941 cgccagtgcc agcagctggt cgcgcatgga ttgcagctcc tcaatgtgtc gttcgatctc
     9001 cgccaccttc tccagcgtgc gccgtttgac gtcggcgctg tgccgctgcg ggtcgttaaa
     9061 cagattcacc agctcgccgc tctcttccag gttaaagccc acctgccgtg cctggcgcag
     9121 taaggtcagt tcgttgagat gctgctgcgt gtaggtgcga taaccgtttt cgctgcgcat
     9181 cggcggcgtc accagcccct tctcttcata gaagcgaatg gctttgctgg tcaggccggt
     9241 aatttttgct acatcgctga tgttcatcgt tcgcgcaacg cctcctgtgc tttgttaaag
     9301 ggtttcaaca gatattcaag cacgtttttt tgccagtgcg aatatcgacc gccaccacaa
     9361 taatacagga agtactgcgc acagagagat gttatacggt tttattgcgc ttcatgacca
     9421 ttgccacaat aaggctgagt aagagtgagc caataatcca tatcaacgca cggctaccct
     9481 gggaccacag actgtagata aagccaacga tcaccaacaa catcaccaac gtaccaacga
     9541 taagaatcgg caacgaggcg tgaatatcat gacgcaggcg gatcgccacc acaaacaccg
     9601 ccaggtagca aatcaaaaag gtggcgctgg caacgctggc gagtgagcct aaattcagtg
     9661 ccgccgtcat cagcataatc aacaccacga caataatgtt gccccaggta ctctgccgcc
     9721 acagggattt attcattagc ttcggcagtt cgcgttcgct gcccatgttg tccatgatgt
     9781 taaacacggc gaacaggttc gcgttaatgg ctgaagccgt cgccagtaaa gcgccgatga
     9841 cgacgatcac ataacccaca tgcccgagca gcggagaagc agcctgcgct acggcggtat
     9901 cggcatattt ttctaactct aatgccgata catcgctaag caaaaccagt gccagcgaga
     9961 tataaagcaa cgtggtaacg ccaatcgcca ccagaaacgc ccgtggcata atgacctgcg
    10021 gatctttcac tttatccgcc gcgttcgcca tcatgccaaa gcccgcatag gcaaggaaag
    10081 ttatcccaat acaggagaag aacgcaccgg agctgggggg cgcagagacg gaaatatgcg
    10141 ccggttgcag cgaccagaca ccggcaataa tcagcaataa caggatcatc attttaatgc
    10201 cgacgaggat cacttccagc cgccctaccg catggttgct taaggagttg aaaagcgtca
    10261 tcaccgcaat gatccccaac gcgtagagca aaataaggtg ctcctcctgg ctgccttcat
    10321 gcaaaaactg cacggcataa gcgccaaaag cacgggcgac catggcgatg ctcaccgcca
    10381 gcgtcaacag gtacagtaac gagagcgcca gcgaaaagac gccgttgcct aatccgcgac
    10441 gaaagaagtc gataatgccg ccattgctgg gatagctcgc ccccagacgc gcataggcat
    10501 aaccggaaaa catcgccaca ataccgccaa aagcaaaggc gacccaggtc gaggcttcca
    10561 ttagcaatgc agcctgcccc agca
//
LOCUS       DBBGLQ010000133        10498 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.133, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000133 DBBGLQ010000000
VERSION     DBBGLQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.133"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..237
                     /locus_tag="ACIYSB_004622"
     CDS             <1..237
                     /locus_tag="ACIYSB_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGC5431514.1"
                     /translation="INRTEVHPREVIKRALYHNAAAVVLAHNHPSGEVIPSKADRLIT
                     ERLVQALALVDIRVPDHLIIGGSQVFSFAEHGLL"
     gene            324..545
                     /locus_tag="ACIYSB_004623"
                     /pseudo
     CDS             324..545
                     /locus_tag="ACIYSB_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
     gene            625..993
                     /locus_tag="ACIYSB_004624"
     CDS             625..993
                     /locus_tag="ACIYSB_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGC5431515.1"
                     /translation="MSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRACIRGRFSDADSYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            1083..1460
                     /locus_tag="ACIYSB_004625"
     CDS             1083..1460
                     /locus_tag="ACIYSB_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGC5431516.1"
                     /translation="MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPGAKQ"
     gene            1457..1624
                     /locus_tag="ACIYSB_004626"
                     /pseudo
     CDS             1457..1624
                     /locus_tag="ACIYSB_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            1682..1879
                     /locus_tag="ACIYSB_004627"
     CDS             1682..1879
                     /locus_tag="ACIYSB_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGC5431517.1"
                     /translation="MMPLTTETALDILIAWLQDNIDCESEIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLYQNR"
     gene            1964..2806
                     /locus_tag="ACIYSB_004628"
     CDS             1964..2806
                     /locus_tag="ACIYSB_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGC5431518.1"
                     /translation="MSEITAPRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLTRQ
                     LAEISAMTESIDGKAALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARETWYRSLEYDNFPEISEANILSTFKQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIINNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIRLDDHIRSVQGKECYEDEMFSIRYFKKGSAHIRFRKPELVDRLNDIIAKHYPG
                     VLPS"
     gene            3044..3205
                     /gene="rhuM"
                     /locus_tag="ACIYSB_004629"
                     /pseudo
     CDS             3044..3205
                     /gene="rhuM"
                     /locus_tag="ACIYSB_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            3602..4786
                     /gene="setC"
                     /locus_tag="ACIYSB_004630"
     CDS             3602..4786
                     /gene="setC"
                     /locus_tag="ACIYSB_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HGC5431519.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            4897..5820
                     /gene="yicL"
                     /locus_tag="ACIYSB_004631"
     CDS             4897..5820
                     /gene="yicL"
                     /locus_tag="ACIYSB_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGC5431520.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHK"
     gene            complement(5824..6642)
                     /gene="nlpA"
                     /locus_tag="ACIYSB_004632"
     CDS             complement(5824..6642)
                     /gene="nlpA"
                     /locus_tag="ACIYSB_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGC5431521.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            6864..7157
                     /gene="yicS"
                     /locus_tag="ACIYSB_004633"
     CDS             6864..7157
                     /gene="yicS"
                     /locus_tag="ACIYSB_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGC5431522.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(7198..8388)
                     /gene="nepI"
                     /locus_tag="ACIYSB_004634"
     CDS             complement(7198..8388)
                     /gene="nepI"
                     /locus_tag="ACIYSB_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGC5431523.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(8599..9051)
                     /gene="yicN"
                     /locus_tag="ACIYSB_004635"
     CDS             complement(8599..9051)
                     /gene="yicN"
                     /locus_tag="ACIYSB_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGC5431524.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(9104..10438)
                     /gene="adeQ"
                     /locus_tag="ACIYSB_004636"
                     /gene_synonym="yicO"
     CDS             complement(9104..10438)
                     /gene="adeQ"
                     /locus_tag="ACIYSB_004636"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HGC5431525.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAIVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
BASE COUNT         2808 a         2684 c         2386 g         2620 t
ORIGIN      
        1 atcaaccgca cagaagtcca tccccgtgag gtgattaaac gcgccctgta ccacaatgcc
       61 gctgccgtgg tgctggcgca caatcacccg tccggtgaag tcatccccag caaggcagac
      121 cgccttatca ccgagcgtct ggtacaggca ctggccctgg tggatatccg ggtaccggac
      181 catctgataa tcggtggcag ccaggttttc tcctttgcgg aacacggtct gctttaaccc
      241 gtcaccgtca caatcacctt catatcactt cagtttctct ttctcagctg tttcttactt
      301 tcacattcag gaggactatt ctcatgaaaa tcatcacccg tggtgaagcc atgcgtattc
      361 accgtcagca tcctgcatcc cgtctttttc cgttctgtac cggtaaatac cgctgacacg
      421 gtagcacgga tacatatacc ggccgtgaag tacaggatat tcccggtgtg ctggctgtgt
      481 ttgcagaacg ccggaaggac agttttggtc cgtatgtccg gctgatgagc gtcaccctga
      541 actgagtctg gaagggcatt cagaagagca gaattatcgc caccaccgga ccattcttaa
      601 ccaattttct gtgaggattt tatcgtgtca gacacactcc ccgggacaac gcttcccgac
      661 gacaataacg accgcacctg gtggggactg ccctgcaccg tgacgccctg ttttggtgcc
      721 cgtctggtac aggaaggcaa ccggttgcat taccttgccg accgcgcctg tattagaggg
      781 cggttcagcg acgcggattc gtaccacctg gaccaggcct ttccgctgct gatgaaacaa
      841 ctggaactca tgctcaccag cggtgaactg aatccccgcc atcagcatac cgtcacgctg
      901 tatgcaaaag ggctgacctg cgaagccgac accctcggca gttgtggcta cgtttatatg
      961 gctgtttatc cgacgcccga aacgaaaaag taactctcca gaataacctt ctgctccggc
     1021 ctggtgcttt caccacgcca ctgtcccatt ttttatctct gcatatcagg aaaatcttca
     1081 ttatgaaaac attacccgac acgcatgtac gggaggcatc gtgctgcccg tctcccgtca
     1141 ccatctggca gacactgctc acccggttac tggaccagca ctacggcctc gcgctgaatg
     1201 acacaccgtt cgctgatgaa cgtgtgattg agcagcatat tgaagcgggc atttcactgt
     1261 gtgatgcggt gaactttctc gttgaaaaat acgcgctggt acgtaccgac cagccgggat
     1321 tcagcgcctg tacccgttct cagttaataa acagtattga tatcctccgg gcacgccggg
     1381 caaccggcct gatgacacgc gacaactaca gaacggtaaa taacattacc ctgggtaaac
     1441 atccgggggc gaaacaatga aactggccct gacgttggaa gccgacagca ttaatgcgca
     1501 ggcgctgaac atggggcgca ttgtcgttga cgtcgatggt attaagctcg ctgaactgat
     1561 taacaaagtc gctgaaaacg gttattcact ccgcgtggtg gatgaacatg acgctaccga
     1621 aacaccggcg acttctgcca gccctcatca gttgccggaa gacaatacag tgatacagag
     1681 cgtgatgcca ttaaccactg aaacagcgct ggatatcctc attgcgtggc tgcaggacaa
     1741 tattgactgc gaatcggaaa ttatctttga caacgatgag gacaaaacgg attcggcagc
     1801 actgttgccc tgtattgaac aggccaggga ggatatccgt accctgcgcc aactgcagct
     1861 tctgtaccag aaccggtgag tcttactcac cagacttcat tccactgacg ccagcctgaa
     1921 cagggcaggc gttttcattt atctgcaaaa aggaatatcg attatgtctg aaatcacagc
     1981 cccccgtccg gaagtggtca acgggcatac ggacgttatc tgctcaacca gtatccgcca
     2041 cattctggct gtacgaaaga gtacgctgct gcaaatcgac acacttaccc ggcaactggc
     2101 agaaatctca gcaatgacag aaagtattga cggtaaagcc gcactggact gggcgatgaa
     2161 acaggatttt cgctgtggtt gctggctgat ggaaaaacct gaaacggcaa tgaaagccat
     2221 cacccgcaat ctcgatcgcg aaatctggcg tgacctgatg caacgttccg ggatgctgtc
     2281 attaatggat gcgcaggccc gtgagacatg gtaccggtca ctggaatacg ataattttcc
     2341 ggaaatcagc gaagcgaaca ttctgagcac atttaaacaa ctgcaccaga acaaagatga
     2401 agtgtttgag cgaggggtga tcaacgtctt caggggactg agctggaatt acaaaactaa
     2461 ttgcccctgt aaatttggca gtaaaattat cattaacaac ctggtaaggt gggaccggtg
     2521 gggatttcat cttatcaccg ggcaacaggc tgaccgactc gccgatctgg aaagaatgtt
     2581 gcacctgttc agcggtaaac cgatccccga caaccgggaa aacatcacta ttcgtctgga
     2641 tgatcacatc cggtctgttc agggtaaaga atgctatgaa gatgagatgt tcagcatcag
     2701 atactttaag aaaggctccg cgcacatcag gttcaggaaa ccagaactgg ttgacaggct
     2761 gaatgatatt attgcgaaac actatccggg agtgttacct tcataataaa gctctaagaa
     2821 ttgattcgat tgtgtatttt agcttcattc ccttacacaa agttatttgg gggttcttat
     2881 gggggttact catttatcca acacagatta atcatttaaa atcaaaacat taacactcat
     2941 attcgactcc tgtgatcttc cgccaaaatg cctctcctga cgttctccaa ccccttgtaa
     3001 tactgtcaat ccagatattt tctactgctc aatattcaat cggcttcagg aatacctggt
     3061 ctaaggtttt gtgatggacg atgagcggct gaagaatccg cctgtcggtt cttctgtcct
     3121 cgaacgcatc cgcgatattc acgccagtat gcgccgtgtt tatttgcagg tgatttgccc
     3181 ccctgctgac tctcagccaa ctgtatcgcc attaaggtga agatgttgtt ttactcgccc
     3241 cagaacaccc ttttttatac gactattcta tttctttaac cgctggcgaa aaaaactgac
     3301 tataaacttc accaataatc agactgccat caagattaat ttgttcattt aatatatcaa
     3361 atcaacttgt tttattttta ctatcaaaaa tagctcattg ccttactata attaatatat
     3421 aaattcggta attaattctt aacatgcttt tactaataat ctcaattgct ggccctataa
     3481 tattgcgcta gcattgcttt ctggttgtat cagcgatact caaaaattct taatacaata
     3541 ctcattcgac tggtacttat ttgtaactca gttatatttt ttcgcccggt gattcagaag
     3601 aatgcaaaaa acggctacca ctccatcaaa aatacttgat ctcactgccg cggcattttt
     3661 acttgtcgcc tttctgacgg gtattgcggg cgctcttcag actcctaccc taagtatatt
     3721 cctcgcagat gaactgaaag cccgtcctat aatggtaggt tttttcttca ccggtagcgc
     3781 tattatggga attctggtca gtcaatttct ggcaaggcac tccgataaac aaggcgaccg
     3841 taaattactg attctgctat gttgcttatt tggagtgctg gcctgcacgc tttttgcgtg
     3901 gaatcgcaac tacttcattc tcctctcaac gggcgtactt ctgagtagtt ttgcttccac
     3961 cgcaaacccg caaatgttcg ccctcgcccg tgaacacgcc gacagaacag gccgtgagac
     4021 ggtcatgttc agtacatttt tacgtgctca gatctcgctt gcctgggtta tcgggccacc
     4081 gctcgcttat gaactggcaa tgggatttag ttttaaagtg atgtatctca ccgctgccat
     4141 cgcatttgtt gtttgcggac tgatagtctg gttgtttttg ccatcaatac aaagaaatat
     4201 tcctgtcgtt acccaacccg tagaaatttt accctccacc cacaggaagc gggatacgcg
     4261 gctacttttt gtggtctgtt caatgatgtg ggcggcgaat aatctctaca tgataaatat
     4321 gccgctattt attattgatg aactgcatct aaccgataaa ctggctggag aaatgattgg
     4381 tatcgctgcc ggtctggaaa ttccgatgat gttaatcgca ggctattaca tgaaacgtat
     4441 tggcaagcga ctattaatgc tcattgctat cgtgagtgga atgtgttttt acgccagcgt
     4501 actcatggcg acgactccgg cggttgagct ggaattgcaa attcttaatg ccatcttcct
     4561 tggtattctc tgtggtatcg gcatgcttta ttttcaggac ttgatgcctg aaaaaatagg
     4621 ctctgcgaca acgttatatg caaatacttc acgcgtcggc tggattatcg ccggctctgt
     4681 tgacggaatt atggttgaaa tctggagcta ccatgcgttg ttctggctgg cgatagggat
     4741 gttgggtatt gcgatgattt gcctgctgtt tattaaagat atttagctca ccagtctggg
     4801 ctaagaaaaa tcggcgtggc gcaaacagag ccatcctcgc catttttgct atcatgcctg
     4861 catacataaa cgacaaaaca gtatgcagag ggaaaaatgg gttccaccag aaaggggatg
     4921 ctgaacgttc tgattgccgc cgtgttgtgg ggaagttcag gggtctgcgc gcaatacatc
     4981 atggagcaaa gccagatgtc gtcgcagttt ttgactatga cgcgtttgat attcgccggt
     5041 ttgattctac tgacgctgtc atttgttcat ggcgataaaa tcttttctat tattaacaat
     5101 cataaagatg ccattagcct gctgattttt tccgtggttg gcgcgctaac tgtacagctc
     5161 acttttttgc taaccatcga aaaatcgaac gcagccacgg caacggtgct gcaattcctc
     5221 tcaccgacga ttatcgtcgc ctggttctca ctggtgcgta aatcgcgccc gggcattctg
     5281 gttttctgcg ctattttgac atcgctggtc gggacttttt tattggtgac acacggtaat
     5341 ccgacgtcat tatcgatctc tcctgccgcg ttgttctggg gcattgcctc ggcatttgct
     5401 gctgcattct ataccaccta tccctcaacg ctaattgccc gctatggcac gttaccagtc
     5461 gtcggctgga gtatgctgat tggcggtctg attctgttgc ctttttatgc cagacaagga
     5521 acaaactttg tcgttaacgg cagtttgatt ctggcgtttt tttatttggt ggtcattggt
     5581 acgtccctga catttagtct gtacctgaaa ggagcacaat taattggcgg tccaaaagcc
     5641 agcattttga gctgtgcaga accattaagt agcgcgctac tctctttgct gttgctgggg
     5701 atcacgttca cattaccgga ctggctggga acgctgctga ttctgtcatc ggtgattttg
     5761 atttcaatgg attcccgtcg ccgcgccaga aaaataaatc gtccggcgcg gcataagtga
     5821 gaattaccag ccaggcaccg cgccaccgtt aaaaatggtt tctgccgctt tagcgacttc
     5881 gggtgattga taagattgca gaaattcctt cacgttttct gcattcttat tatcttcccg
     5941 tgccaccaaa atattcacat acggcgaatt cttatcttca ataaatacgc tgtcgtgcac
     6001 cggagaaagc ccggtctgct gaatgtaagt ggtgctgata atcgctacat caactttggg
     6061 atcatccagt acgcgcggga gctgcgcccc ctcgagttcc ataatctgca aatgacgagg
     6121 attatcggtt atatccagcg cggtaggtaa taaccctttt ccctctttca gagtaattag
     6181 cttctctttt tgcaacagta aaagtgcgcg accaaggttg gtcgggtcgt tggggatcgc
     6241 cactgtcgcg ccttctttta tttgcgccac ggttttgatt tttttggaat aacccgccat
     6301 agggaagaca aacgtatttc ccaccgctac cagtttataa ccatgcgcct gattatcttg
     6361 ttcaagaaag ggtcgatgct ggaagacatt cgcatcaagt tcgccatgat ttgtcgcatc
     6421 gttcggtagc aacgaaccgc taaatcctac cagttcaaca tcgaggccat atttctcttt
     6481 tgccaccttt ttagcgactt ccgcgacatc ttgttctgcg ccatttataa cgccaacttt
     6541 aatgtgtttt gcatcgctgc tactctggtc gcaacctgcc agcagaattc cggccagcaa
     6601 taatgcggcc cctgtccgta gatgatgtgt tgtcagtttc atttttttat ccttttatta
     6661 atgattacct gactatagcg gtaaggtcgc tgcggtttaa aaaacgaaac gctatcgata
     6721 agaataaaaa ggaataaaag tggaatataa agtctggcac ttcttactga ccacgcaagc
     6781 gcgtttcgta cagcatgatg agagcgatga aagcaaacta cacttatgct tcattcgata
     6841 cacatttgta aaaggttgaa tccatgaaac caacgacact actgcttatt ttcacttttt
     6901 tcgctatgcc aggcatcgta tacgccgagt cgccttttag ctcactgcag tccgcaaaag
     6961 agaaaaccac ggtattacaa gatttacgta aaatttgcac gccacaggcg tcattatcag
     7021 atgaagcgtg ggaaaagtta atgttgtcag atgaaaacaa taaacaacat atacgcgagg
     7081 ccatcgtggc gatggaacgt aataatcaga gcaattattg ggaagcgttg ggtaaggtag
     7141 aatgcccgga tatgtaagaa cagcaacaac tactgaccaa cgcttcttta cctgatatca
     7201 ggatttcttc attttcacct ttgcagtaac caacaatgca gtcagcaaca tcaatgtgcc
     7261 ggacaacatc aacggcgaag tcagaccaat attatccagc gcataaccgc cgattgccgc
     7321 gccacaggta ttagcaagct gaataaccgc cacctgaata gacccggctt tttctgcctg
     7381 atcggccagc gagcgggtga tccacgttga ccagccgacg ggaaccaatg caaaagttag
     7441 cccccagata atcgccacgc cggtagcaac gattttatcg cttccccaca acgtcagtac
     7501 caacgcactc acagccagta ttaacggcgc gcctgctaag gccagtttta ccgaacgttt
     7561 aagaatgaac gacgaaagcg acgtaccaat aaagctggcg ataccaaaac tcaacagcac
     7621 cagcgttaag ccatccacgc cgaatcccgc caggttcata tacaccgggc gaatatacgt
     7681 gaagaaagca aactgcccgg cgaaagacat gaagatggcg atcatccctg ccatcacacc
     7741 cggacgttgt aataagcgga aagtattttg tttctgatgc gagggttcgc ctggcagtga
     7801 aggcaatgat ttgataatcc agaaaataca cagcacgccc atcaccgccg ccgcattaaa
     7861 gacattgcgc caaccgataa gctcgcctaa aaaactgccc aacggcgcgg caatcaccag
     7921 cgcaatagaa accgcgccga agatcaccga cagcgccttc ggcaccgtac gcggcggcac
     7981 cagacgcatg gtcagcgacg ccgacatcgc ccagaacccg cccagcgcca gccccagaca
     8041 ggcacgaccg attaaaagca aactgaatga gttagcaaag gaaaccagca agcaggagag
     8101 cgtcagcaaa acggcaaaca aaataacaac gtagcggcgg tcagtagcct gaattgtctg
     8161 ggtaataaac aaactggcaa acattgccac aaaggcggtc acggtcaccg attgcccggc
     8221 aaccccttcc gaaatgccta aatcctgggc cattggcgtc aacaaactga cgggcaaaaa
     8281 ctcaacgata atcagacagg cgacacaaaa cgccaccgag aaaacggctg accagttcgg
     8341 tcgggtgatg gcatccgcgc cgcggttttc ggcaataaat tcactcatgg tgttacccgt
     8401 ggcatgtttc aggggaaaag ccgaccactt taacattgaa atgtgtgacg catttaacgt
     8461 ttttgcaact tttacggtgt tgcttgagct ggagttaaga tcgaaacgga acagggcaaa
     8521 gattaccgga tgcggcgtga acgtcttatc cacccgacag gtcaggcgcg gtctgtaggc
     8581 tgataagacg aaacggtttc aatgcaacca gtgaacgccc tcggcaggct gaaagaacgc
     8641 gttatagcgc atctgaaaag cattaatatc ctgcatttca ggttcggttt cgcgcagaaa
     8701 acctagcgcc agcctgacct gagcgtcggt aatcggcgcg gcgaggcgag ctttttgtaa
     8761 aatccgatgc cattgcagca ggttctgttc gctaccatcg acaatgacaa tttgccagat
     8821 gagcaacacc tgcgcggcgt tttgcaggtg cgactcatcc ggacgatgca aataacgtaa
     8881 aaattcgtca ccagctgact ttcccatttg atcgaccatc gactccacgg gtacgacatc
     8941 gatgttcagc cgatcgctga gacggcgaat cgcttttctg gccccggatg tcagcacccg
     9001 aaaaccaacc acaaacacta ccgccagcgt ggcgagcatt atccagatca tgagttctcc
     9061 tgcgtaatca gaactcatga tatctggaaa ccctcgccag ttactaatcc accagaataa
     9121 tcttcagtgc aaacagagct gcgaccacca ccacacacag gttcagatcg cgccagcgcc
     9181 cggtgcatac tttcatgatg cagtacgaca taaagccgag tgcaatccct tcggtgatcg
     9241 agaaagtaaa gggcatcatc accgtggtaa taaacgcagg caccgattcg gtaaaatcat
     9301 cccagttaac gcgcgccagg ctcgaagtca tcagcacgcc aacaaagatt aacgctccgg
     9361 cggttgcgta aggaggaact atcgccacca gcggtgagaa gaacataacc aacaggaaca
     9421 taacgccaac cacaaccgca gtcagccccg tgcggccacc gactgccaca ccagaagtac
     9481 tttcaatata ggcagtaaca gacgaggtgc cgataaacgc acccgccacc gaactgacgc
     9541 tatcaacata cagcgcctta ttcatattgg ggaatttacc gttaccatct attaagcccg
     9601 ctttatcagt tacaccaatt aatgttcctg atgaatcaaa taggttgatc agcataaagg
     9661 agaaaatgat accggcgagt tcaagtgtta acgcgccgct caaatctact tcaccaatga
     9721 cgccgctaat atcaggcgga atggaataga cgccgctaaa atgaacatca ccgaaaaata
     9781 atccacagca agacgtcacc acaatagaaa ccagcaccgc ggcatgaaaa tgacgtgatg
     9841 acaacacggt tataataaaa aaccctaaaa tacctaacaa cacgccgtga gaacttaaat
     9901 cgccaatcat caccagcgtg tctttattgg cgacaataac gccagtattt tttaatccca
     9961 ttaaggcgat aaataatcca attccgctgg tgataccaat acgtaaactt aagggaatgt
    10021 tggagatcat ccagtaccgg atacgaaaca gcgtgagcaa aaatagtcca actgcgcccc
    10081 agaatattgc gcccatcccg gtctgccagg agatgcccat cgcccccacg accacgaagg
    10141 caaagaaggc gttcagcccc attgccggag ccagcgccac gggtaagtta gcaaatatcc
    10201 ccatcgcaat actgccgata ccggcaatca aacaggtggt aacaaacacc actttcgggt
    10261 ccatttgtgc cgcgccgagg atttgcgggt tcacaaaaac gatgtacacc atggttaaaa
    10321 aagtggtcat ccccgcaatc aattctgtgc ggacggtggt cccatgctga ggtagtttaa
    10381 ataatcgcga aagcgtccct gattcattac tcacgtaatc ggtattgtca ttattcattt
    10441 ttttgtccat tggaggagat ttaatccctt gcttttgtaa aacaaatgac aacaacgg
//
LOCUS       DBBGLQ010000134        10434 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.134, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000134 DBBGLQ010000000
VERSION     DBBGLQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.134"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..127
                     /locus_tag="ACIYSB_004637"
     CDS             <1..127
                     /locus_tag="ACIYSB_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGC5431526.1"
                     /translation="CKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKERRKEWGIL"
     gene            112..750
                     /locus_tag="ACIYSB_004638"
     CDS             112..750
                     /locus_tag="ACIYSB_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGC5431527.1"
                     /translation="MGDSVTAEVLSDMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKHSLEQDGFTQPVVVSEEKEHYLV
                     VDGFHRQLLGREAGTGKRLKGWLPVTCINPERKGQAARIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTDERIGMELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            829..1098
                     /locus_tag="ACIYSB_004639"
     CDS             829..1098
                     /locus_tag="ACIYSB_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGC5431528.1"
                     /translation="MVAQTLEAAGCWRRASARWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIASPSGEVFRKGTLVC"
     gene            1119..1763
                     /locus_tag="ACIYSB_004640"
     CDS             1119..1763
                     /locus_tag="ACIYSB_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431529.1"
                     /translation="MMKKVMVDSVSVPWQLLCDEHGFTREHHNQLKKFSPEALREIIA
                     EIACCHPSTSVLLRNKWLTPPEDIPEQVTREYERRIQNCPSFRSEKEAESWLNELFFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQRQRITKILNESDTKRVAEEIKFRIQYLQDEEQKRVLSELVNVLTSKR"
     gene            1829..2152
                     /locus_tag="ACIYSB_004641"
     CDS             1829..2152
                     /locus_tag="ACIYSB_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431530.1"
                     /translation="MSNRYWMISFHKNTRNLMVRSVPKEDIVHNIELIIRSLNIFIEK
                     FLSIEYINVEGIPLTSRRITIMGLKPGPKPIAKSTGKPDQRRRDNKDTPGNTPGLKPS
                     KSTGK"
     gene            complement(2771..2939)
                     /locus_tag="ACIYSB_004642"
                     /pseudo
     CDS             complement(2771..2939)
                     /locus_tag="ACIYSB_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            3186..6230
                     /locus_tag="ACIYSB_004643"
     CDS             3186..6230
                     /locus_tag="ACIYSB_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HGC5431531.1"
                     /translation="MKFTEDTRVKIPVILHLIRLGYYYLSLKEQRWDKETNIFPDLFT
                     AAIGRINPGLAPDDIGRLLKDLTLLLDNDDLGRAFFEKLSDRSGIKLIDFDNFNNNSF
                     HVVTELTCENDDEEFRPDITLLINGMPLIFIEVKKPNNREGIVAERNRINERFKNPKF
                     KRFANITQFMIFSNNMEYDDSDPEPIQGAFYASSSYSNPVFNYFREEELFNLTNLLKP
                     LSDETEIAVLKDNNLEVIRTNPEFQTNKDHNCPTNRLCTALLSRDRLAFILRYALVYV
                     AEPGGIQKHIMRYPQLFATKAIERKLNAGVKKGIIWHTQGSGKTALAYYNVRFLTDYF
                     QNQQVIPKFYFIVDRLDLLQQAQREFTARGLIVHLIDSREAFTRDIKATQALHNHHGK
                     AEITVVNIQKFRDDPDVVTTKDYDINIQRVYFLDEVHRSYNPKGSFLANLSQSDTNAI
                     KIGLTGTPLLGTDYNSRDLFGDYIHKYYYNASIADGYTLRLIREEIATNYRMILQQAL
                     KEVEVRMGDVDRKDIYAHPSFVKPMLNYIITDFAKSRGALNDDTIGGMVICDSSEQAK
                     QMFEIFNTDYAESPTTVYHIHTKETRKIKRAALILHDVGSKQERKEWVEQFKAGEIDL
                     LFVYNMLLTGFDAKRLKKLYLGRVIRKHNLLQALTRVNRTYNHFRYGYVVDFADIRQE
                     FDATNKAYFEELQAELGDEMEHYSRLFKSADEIREEIEHIKDVLFSFDTENAEIFTDQ
                     INQIQDRETALVLKNALTDARSLYNLIRLQGNTEFLQALDFNKLNILYREASRRLDLL
                     NLKADLVQGVDLSGLLNRALEEVIFEFHKVGEEELVLADELKETLRRTREALAANFDQ
                     QDPQFVSLKDELERLFKKKKLSEVTQQEMVANIGTLNKIHGRIKELNRQNNLLRQKYR
                     GDVKYARTHKRLRERGGLSEPERKIFEALLSVKLDADEKVLNNSQILNNESYFERHME
                     SCVITHFEDEHHITLTSSAVSDINRLIVAEYLNEFNTGARTW"
     gene            6224..7858
                     /locus_tag="ACIYSB_004644"
     CDS             6224..7858
                     /locus_tag="ACIYSB_004644"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HGC5431532.1"
                     /translation="MVELEFRDKTKALIDSLKSICANYGLGNDGNEFKIITQAFLYKF
                     LNDKFAFEAKQKDKSIASAESWEDALSAMSEDQLKKLQQRMAPDTARLKPHHFIRYLY
                     NRQNAADFARTFDDTLMDIAATNNDVFAVKTDGGAKVVLFERLSQYIADESKRDDFCR
                     AIINKLADFSFERIFTQKFDFYATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMAEIL
                     VPKAQQGVVRNVSCYDPSAGSGTLLMNVAHAIGEDRCSIFAQDISQKSSSLLRLNLIL
                     NNLVHSIPNVIQGNTILHPFHKDGSALKRFDYIVSNPPFKMDFSDFRDDLDSKENQLR
                     FFAGIPKVKAKAREKMEIYQLFLQHIIFSLKPDGKAAVVVPTGFITAQSGIDKGIREH
                     LVKNKMLAGVVSMPSNIFATTGTNVSILFIDASNKEKVVLIDASNLGAKVKDGKNQKT
                     VLTEEEEQRICEVFNNKWSEEDFSVVVSYDDIAGKNYSFSAGQYFDVKIEYADMTPEQ
                     FAEKMKGFTDNLESLFSQSLELEAEIKMQMAGLKYE"
     gene            7851..9083
                     /locus_tag="ACIYSB_004645"
     CDS             7851..9083
                     /locus_tag="ACIYSB_004645"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGC5431533.1"
                     /translation="MSNAVSFLKYGDVAEFRNGLNFSKDSHGKGCKFIGVADFKDNFT
                     PQWKLLGEINPFGVAKREDYLEPGDIIFVRSNGNKALVGRSLYIDLNEKSLYSGFCIR
                     ARLKTDDFLPLFLAYYTRTNFFKSAITSVAGTNINNLNQDILGNIMIPHYSIGIQKSI
                     VAVLTSIDEKIAINNRINAELEAMAKTLYDYWFVQFDFPDANGKPYKTSGGRMEYNAT
                     LKREIPAGWAVNTLSQIANITMGQSPAGESYNEDGIGTLFFQGSTDFGWLFPTPRQYT
                     TSPTRMAKKGDILLSVRAPVGDMNIANADCCIGRGLAALNSKSRSDGFLFYVMKYFKQ
                     VFERRNAEGTTFGSMTKDDLHSLQVVCPEPGLLKRYDDIVSEYNKMIFTRSLENQDLI
                     KLRDWLLPILMNGQVKIK"
     gene            complement(9324..9425)
                     /locus_tag="ACIYSB_004646"
                     /pseudo
     CDS             complement(9324..9425)
                     /locus_tag="ACIYSB_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(9789..9920)
                     /locus_tag="ACIYSB_004647"
     CDS             complement(9789..9920)
                     /locus_tag="ACIYSB_004647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431534.1"
                     /translation="MTEQKITDIAQAKTNFYLFSINARGNHAGKIKLSHNQLLNWLV"
     gene            10145..>10434
                     /locus_tag="ACIYSB_004648"
     CDS             10145..>10434
                     /locus_tag="ACIYSB_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HGC5431535.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERN"
BASE COUNT         2931 a         2475 c         2514 g         2514 t
ORIGIN      
        1 ctgtaagact ctcataaaga atgatttctg gtgccggacc ctctccttca gtcctaacaa
       61 accccggcac tatgaacgtt atctgcagcg tatgaaagaa aggaggaagg aatgggggat
      121 tctgtgacag cggaggtact gagtgacatg attcgtcagt acttcagcca ggaacgacca
      181 gaagaagaga ccatacaggc tctgaatcat ctgcgcaggg tgcttcatga ggtcagtcca
      241 tttgcacagg agcctgtgga ctgtgtgctg tgggtgaaag cagatgaggt ggtggccaat
      301 gattataatc ctaatgtcat ggcgccgggt gagaaaaaac tgctgaaaca ttcgctggag
      361 caggacgggt tcacgcagcc tgtggttgtc tcggaagaga aggagcatta tctggtggtg
      421 gatggttttc atcgtcagtt actgggcagg gaagcaggta cgggaaaacg tctgaagggc
      481 tggttgccag tgacctgtat taatccggag agaaagggac aggccgcacg cattgccgcg
      541 accattcgac ataaccgggc caggggaaaa caccagataa cttcaatgtc tgatattgtc
      601 agggatcttt cacgtctggg ctggacggat gaacgcattg gcatggagct gggtatggac
      661 caggatgaag ttctcaggtt gaaacagatt agcgggctga cggagctgtt tcaggaagag
      721 gatttcagcc cggcatggac ggttcgctga gactgtcaga tatgtgtatt atttcagtga
      781 gttgatgttt tgagcaggaa aaatagtacc ccggaagtcg tggaagacat ggttgcacag
      841 acgctggagg cggcgggttg ctggcgcaga gcttctgccc gctggctttt tgttatggga
      901 aatgttgaat gtacagaagc acaacgggag tggttgttgt tacgcaggaa ttattgcctg
      961 gcgcagatat cgtccccgcc gctacctgaa aagctggata tcagcgaggt ggcgaaagct
     1021 gcagatgcca cgctgaggcg aatggggatt gccagtccgt ctggggaggt attcagaaaa
     1081 ggtacactgg tctgttgata ttcaggtaac aggtattaat gatgaagaaa gtaatggtgg
     1141 acagcgtatc agtgccatgg cagcttttgt gcgatgaaca tggttttacc cgggagcacc
     1201 acaatcagtt gaagaagttc agcccggagg cattacgtga aattattgct gaaatagcct
     1261 gctgccatcc atcaaccagt gtattgctca ggaataagtg gctgacaccg ccggaggata
     1321 ttccggagca ggttacccgt gaatacgaac gaagaataca gaactgtcca tcgttcagga
     1381 gcgagaagga agcggaatcc tggttaaatg aactgttttt tgctgtgatt gctccgctat
     1441 accgtgttgc ccccgaacaa acagaacagg ttgagtcctt tgttctgcag cttattgccg
     1501 agcaggagcg ggtttgggag cttattttaa ccaatgatgg ttattcatgg cattgcgcac
     1561 tttatgatat attatttcat ctggttttac gaaatatgga agaccagcct gaacatcaga
     1621 gacagcgaat aacaaaaata cttaatgagt ccgatacaaa acgggttgca gaagagatta
     1681 aatttcggat tcaatatctt caggatgaag aacaaaaaag agttttaagt gaactggtaa
     1741 atgtcctgac ttcaaaaaga taaaacatgc cggagtaatt ttttctatat gttaataaaa
     1801 tcggtgcgta ttaccatgaa ggagatcgat gagtaaccgt tattggatga tctccttcca
     1861 taaaaatacg cgtaatctaa tggttcgctc tgtaccaaaa gaagacattg ttcacaatat
     1921 cgagttaatc attcgatcgt tgaatatttt cattgaaaaa tttctcagta tagaatatat
     1981 caatgttgag ggtattcctc ttacatctcg aaggataact atcatgggtc ttaaacctgg
     2041 tccaaaacca attgctaagt cgacaggaaa accggatcaa cgccgacgcg ataataaaga
     2101 tactcctggc aatactccgg gactaaagcc aagtaagtcc acaggtaagt aaaaaaataa
     2161 gggtataaat aagcatttat accctgacta tatatccagt ccaccgaagg tgtttcagca
     2221 gccagatgct cgttctttct atatcatacg cctttgcccc gtaccctttc gcaagcaaag
     2281 actctggtaa atactcgtca tatttatcca gatccttatc aggaacaact gtagcaggct
     2341 caaattctga aggtgcaaaa tggcatctga atcgatcacg acttggcatg cttccgatca
     2401 ttgattggca tcctgccaat cgtatcgttc cggcaaactc atattgacgc atttttcagg
     2461 cgagccactg ggagcgcata cgaatgggcg ccgtctgacg atggttgatt tattttgttg
     2521 tacaggggcg atcgccaggc aggtgccgga ggctggctct gaagtgctga gtcgggtaat
     2581 cgcgtcaggc tgcgcagtac tgacatgggt actggtggca ggtgatgaag ttcgactccg
     2641 gaaatgccgc cgggttgatt gcgtattccg ctctattcga tcacggattg gccccctgtg
     2701 tacgaacatc tgacggcagg tgttgctcat acagggtaaa tccctcaaga ggcaacaact
     2761 ttgtcagtcg ctactccggc cactcaggca gacgcgtcag gatgttcgtc agcccggcat
     2821 gtgactctag gccattacgt tttgctgtat ccagaagact cattatctgt gctgcatgtt
     2881 ttccgtccca tgataacccg atggatggtg tgttcgtaga tattgttgca gtatggaacc
     2941 atattctaag atactttgct gtaagaaatg ctacgtttga tgagctctat tttattattc
     3001 tgccagctaa aaattagtat tgaatataaa actcaccaca ttgagaataa atgaaacaaa
     3061 tatatttaat caggaagtta aaaacagata acatgggtgt aatacaataa ggtacagcca
     3121 gtaattccgg gcttgcggag ttgctggatc gtatgactta gacgtgataa aaaacaggga
     3181 atacgatgaa attcactgaa gatactcggg taaaaatccc cgtaatcctc cacctgatac
     3241 ggttgggata ctactacctg tcccttaaag aacaacgctg ggacaaagaa accaacatct
     3301 tccctgacct ctttactgct gcgattggcc gaataaaccc cggacttgcg ccagacgaca
     3361 tcggacgcct gttaaaagac ctcacgctgc ttctcgacaa cgatgatctg ggccgcgcct
     3421 tcttcgaaaa actcagtgat cgttccggta tcaaactgat cgactttgat aacttcaaca
     3481 acaacagttt ccatgtcgtc accgaattga cctgcgaaaa cgatgatgaa gaattccgcc
     3541 cggacatcac gctgctgatc aacggcatgc cgctaatctt tatcgaagtg aaaaaaccca
     3601 ataatcggga aggcatcgtc gcagaacgca atcgcatcaa cgaacgcttt aaaaacccca
     3661 aattcaaacg cttcgccaac atcacccagt tcatgatctt ctccaataac atggagtatg
     3721 acgacagcga cccggagccg attcaagggg cattctacgc cagcagctct tacagcaacc
     3781 cggtattcaa ctacttccgc gaagaagaac tcttcaacct gaccaactta ctgaaaccgc
     3841 tgtcagacga aaccgagatc gccgtcctga aagacaacaa cctggaagtc atccgtacca
     3901 acccggaatt ccagaccaac aaagatcaca actgcccgac taaccgcctt tgcaccgcgc
     3961 tgttgagccg ggatcggctg gcatttattc tgcgctatgc gctggtgtat gtcgccgaac
     4021 cgggcggaat ccagaaacac atcatgcgct acccgcagct cttcgccact aaagcaatcg
     4081 agcgcaaact gaacgctggt gtgaagaaag gcatcatctg gcacacccag ggcagcggga
     4141 aaaccgcgct ggcttactac aacgtgcgct tcctgacaga ttacttccag aatcagcagg
     4201 tgatccccaa attctacttt attgttgacc gtctggatct gctccagcag gcgcagcgcg
     4261 agttcaccgc ccgtggctta atcgttcacc ttatcgactc ccgcgaagcc ttcactcgcg
     4321 atatcaaagc gacgcaggcg ctgcacaacc accacggcaa agcggaaatc accgtcgtca
     4381 acatccagaa attccgcgat gacccggacg tcgtcactac caaagactac gatatcaaca
     4441 tccagcgcgt gtacttcctc gatgaagtcc accgcagcta caaccccaaa ggaagtttcc
     4501 tggcgaactt aagccagtcg gacaccaacg ccatcaaaat tggcctgacc ggtacgcctc
     4561 tgctgggcac agactacaac tcccgcgacc tcttcggcga ctacatccac aaatactact
     4621 acaacgcctc cattgccgac ggctacacgc tgcgcctgat ccgcgaagag atcgccacca
     4681 actaccgaat gatcctgcaa caggcgctaa aagaggtgga agtcagaatg ggcgatgtgg
     4741 atcgcaaaga catctacgcc catcccagct tcgtcaagcc gatgttgaac tacatcatca
     4801 ccgactttgc caaaagccgg ggcgcgctga atgatgacac catcggtggc atggtgattt
     4861 gtgacagctc tgaacaggcc aaacagatgt ttgagatatt caacactgac tacgccgaat
     4921 cgcccaccac cgtttaccac atccacacca aagaaacgcg gaaaatcaaa cgcgccgccc
     4981 tgatcctaca cgacgtcggc agcaagcagg aacgcaaaga gtgggtggag caattcaaag
     5041 cgggagagat tgacctgctg ttcgtctaca acatgttgct cactggcttt gacgccaaac
     5101 ggctaaaaaa actctacctc gggcgggtga tcagaaagca caacctgctc caggcgctga
     5161 cccgcgtcaa ccgaacctac aaccacttcc gctacggcta tgtggtggac ttcgccgaca
     5221 tccgccagga atttgacgcc accaacaaag cctacttcga agagctacag gctgaactgg
     5281 gcgatgaaat ggagcattac tcccgcctgt tcaaatcagc ggatgagatc cgcgaagaaa
     5341 tcgaacatat caaagacgtt ctgtttagct ttgataccga aaacgcggaa atcttcactg
     5401 accagataaa ccagatccag gaccgggaaa ccgcgctggt actgaagaac gcgctgaccg
     5461 acgcccgcag cctttacaac ctgattcgcc tccagggcaa cacagagttt ctccaggcgc
     5521 tggatttcaa caaactcaac atcctctacc gcgaagccag ccgccgcctc gacttactca
     5581 acctgaaagc ggatctggta cagggcgttg acctctccgg cctgctgaac cgggcgctgg
     5641 aagaggtgat ctttgaattc cataaagtgg gcgaagaaga gctggtactg gccgacgagc
     5701 tgaaagagac gctgcgccgc acccgcgaag cgctggccgc caacttcgac cagcaggacc
     5761 cgcagttcgt cagcctgaaa gacgaactgg agcgtctgtt taagaaaaag aaactcagcg
     5821 aagtcaccca gcaagaaatg gttgcgaata tcggcacact caataaaatt catggccgca
     5881 tcaaagagct aaaccgccaa aacaacctgc tgcgccagaa ataccggggc gacgtgaaat
     5941 acgcgcgtac ccacaaacgc ctgcgtgagc gcggcggcct gagcgagccg gagcgtaaaa
     6001 tcttcgaagc actgcttagc gttaagctgg atgcagatga aaaagtgctg aacaacagcc
     6061 agatactgaa caatgaaagc tattttgagc ggcatatgga gtcatgtgtc atcacgcact
     6121 ttgaggatga gcaccatatc actctgacct ccagtgcggt aagcgacatc aaccgcctga
     6181 ttgtggcgga atacctgaac gaatttaata ccggagcacg aacgtggtag aactggaatt
     6241 tagagacaaa accaaagccc tgatcgacag cctgaaaagt atctgtgcga actacggcct
     6301 gggcaacgac ggtaatgaat tcaaaattat cactcaggca ttcctgtaca aattcctgaa
     6361 cgacaaattc gcttttgagg ccaaacagaa agataaaagt atcgccagcg ccgaaagctg
     6421 ggaagatgcg ctcagcgcaa tgagcgaaga tcaactgaaa aagctgcaac agcggatggc
     6481 cccggatacc gcccgcctga agccacacca ttttattcgt tacttgtata accgacaaaa
     6541 cgcagcggac tttgccagaa cttttgacga caccctgatg gacatcgccg ccaccaacaa
     6601 cgacgtcttc gcggtcaaaa ccgacggcgg cgcgaaagtg gtgctgtttg aacgcctcag
     6661 ccagtacatc gccgacgaat caaagcgcga tgacttctgc cgcgccatta tcaacaaact
     6721 ggccgatttc agcttcgaac gcatcttcac gcagaaattc gacttctacg ccactatctt
     6781 tgaatacctg atcaaagact acaacagcaa ctccggcggc aaatacgcgg aatactacac
     6841 gccccacgcc gtggcgcgca tcatggcgga aatcctggtg ccgaaagcac aacagggcgt
     6901 agtgcgcaac gtaagctgtt acgacccgtc cgccggttct ggcacgctgc tgatgaacgt
     6961 ggcgcacgcc atcggggaag atcgttgcag catctttgcg caggacatct cgcagaaatc
     7021 ctccagcctg ctgcgcctga acctgatcct caacaatttg gtgcattcga tccctaacgt
     7081 tatccagggc aacaccattc tgcatccgtt ccataaggac ggcagcgcgc tgaaacgctt
     7141 tgactacatc gtctccaacc cgccgtttaa gatggatttc agcgactttc gcgacgatct
     7201 cgacagcaag gaaaaccagc tacgtttctt tgccgggata ccgaaagtca aagccaaagc
     7261 cagggagaaa atggagattt accagttgtt cctgcaacac atcattttct cgctgaaacc
     7321 ggatgggaag gcggcggtcg tggtgccgac tggctttatc actgcgcaat cgggtattga
     7381 taaaggcatt cgtgaacatc tggtgaaaaa taagatgctg gcgggcgtgg tttccatgcc
     7441 gtcaaatatc ttcgccacca ccggcaccaa cgtgtcgatc ctgtttattg acgccagtaa
     7501 caaagagaaa gtggtgctga tcgacgcctc gaatttaggt gcaaaagtca aagacggcaa
     7561 aaaccagaaa acggtactga ccgaagaaga agagcagcgc atctgcgaag tgttcaacaa
     7621 caagtggagc gaagaggatt tctcggtggt ggtcagctat gacgacatcg ccgggaaaaa
     7681 ttactcgttc agcgccgggc agtattttga tgtaaaaatt gaatacgccg atatgacgcc
     7741 ggaacagttt gccgagaaga tgaaaggatt tactgacaat ctggagagtt tgtttagcca
     7801 gtcgctcgag ctagaagcgg agattaaaat gcagatggcg gggctgaaat atgagtaatg
     7861 ctgtttcttt tctgaaatat ggtgatgtag ctgaatttcg taatggttta aatttcagca
     7921 aagatagcca cggtaaaggg tgcaaattca ttggtgtagc tgattttaaa gataatttca
     7981 ctcctcaatg gaaattattg ggtgaaatta atccatttgg agttgccaaa agggaagact
     8041 accttgagcc aggcgatatt atttttgtcc gttctaacgg caacaaggcc ttagttgggc
     8101 gaagccttta tatagatctt aatgaaaaat cgttgtactc tggtttttgt atccgagcca
     8161 gattaaaaac agatgatttt ctccctctgt ttttggctta ctatacacga actaattttt
     8221 ttaaatctgc tatcacgagt gtggcgggga caaatattaa taatttgaat caagatattc
     8281 ttggtaatat catgattcca cactattcaa taggcattca gaaatcaata gtcgctgtat
     8341 tgacatctat tgacgaaaaa atcgctatca acaatcgcat caacgccgaa ctggaagcga
     8401 tggcgaaaac gctgtatgac tactggtttg tacagtttga tttcccggat gccaacggaa
     8461 aaccgtataa aacttctggc gggaggatgg agtataacgc cacgctgaaa cgggaaattc
     8521 cggcggggtg ggcggttaac acgctgtctc aaatagcgaa tatcacaatg gggcagtctc
     8581 cagccgggga gtcttataat gaagatggaa tcggaacact attttttcag ggttcgacgg
     8641 attttggttg gcttttccct actcctcgcc agtacacaac atcacctacg agaatggcga
     8701 aaaaaggtga tattttactc agtgttcgtg cgcctgttgg ggatatgaat attgccaatg
     8761 ccgattgttg tattgggcga gggcttgcgg cattaaatag caaaagccgc tcagatggat
     8821 ttttattcta cgtgatgaag tatttcaaac aggtatttga gcgtcgaaat gcggaaggaa
     8881 caacatttgg ctccatgacg aaagatgatt tacattcact gcaagtcgtt tgcccagaac
     8941 ccggattact gaaacgatat gacgatattg tttcagaata caataaaatg atttttacgc
     9001 gtagccttga aaatcaggat ctaatcaaac tgcgtgactg gctcctccct attcttatga
     9061 acggacaggt caagattaaa taacccccaa cgtatttttc gaagcgcagt ccaagtttga
     9121 actgcgcttc acttccccca acaaaaaacc tgccatccat tactggtagt gattcctctc
     9181 acattttcca tgtattaatt attattgtta ctactacacc caaacgacct ataatcgtaa
     9241 gcggctcgcc agaaccgtat tgatatttac tgagcgctca gatcaacttt ccagggtaac
     9301 agatcgcgta tccagtttgc ctgtcattcc tagatatgtt caatgacgta acgcagccat
     9361 ttttctggag ccatattgtt cagacggcat gtaccaatcc gcaggtacag catcgccgca
     9421 tcctcaagta atgccggtaa tttggggcgt aattctgttg cgcttttact gaacacaatg
     9481 ccgtattcag taagaagtcc aagaacatga tctatcagtt gtgtccttcc atcgagatca
     9541 accgatgcat tttcaaaaaa gcctggacgt tctgtaggtc cagacttttt attggtacca
     9601 gtcgtatagt tctatattga taggtttcag ctattgctgt gcgtcgttat aatcattttt
     9661 ctgacgccat tgataagatt taacatgttg tgctggatgc aatattacat catagttgag
     9721 ttcccttatt tcctgtgggc agtgatggga tgtcccgcaa gccttcatcg caacggtcat
     9781 tttggctgtt ataccagcca gttaagcagt tggttgtgag aaagtttgat ctttcccgcg
     9841 tgattgccac gagcattgat actgaaaaga taaaagttcg ttttagcctg agcgatgtcg
     9901 gtaattttct gttcagtcat ggcctttctc ttacttttgt ggttaaaatt tattcgctat
     9961 ggcgcaattg aaagcggtcg tgaaggtggg ggaagactat ctcatcactt accatatttc
    10021 cgacatattc tcacaacaaa ataccacgta actattgatg aatattgttc tctcctgagc
    10081 agcgtttatg ctttaattgc taaatcgatt taattagttt agcaattagt atattggagc
    10141 ctttatgagc acaagagtat gggtacttgg tgatgcggtt gttgatttat tacccgaaag
    10201 ccaggggaga ctactacagt gtcctggcgg ggcgcctgct aatgttgcag tcggtatcgc
    10261 aaggctgggg gggaaaagtg cctttattgg caaagttggc gatgatcctt tcggtcgctt
    10321 tatgtatcag acactgagta cagaaaatgt tgatacacgt tatatgtctc ttgatcctca
    10381 acatcgcacc tcaattgtgg ctgtaggact tgatgagcaa ggagaaagaa actt
//
LOCUS       DBBGLQ010000135        10288 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.135, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000135 DBBGLQ010000000
VERSION     DBBGLQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.135"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            177..752
                     /gene="ssuE"
                     /locus_tag="ACIYSB_004649"
     CDS             177..752
                     /gene="ssuE"
                     /locus_tag="ACIYSB_004649"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGC5431536.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            745..1704
                     /gene="ssuA"
                     /locus_tag="ACIYSB_004650"
     CDS             745..1704
                     /gene="ssuA"
                     /locus_tag="ACIYSB_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGC5431537.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPESKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1701..2846
                     /gene="ssuD"
                     /locus_tag="ACIYSB_004651"
     CDS             1701..2846
                     /gene="ssuD"
                     /locus_tag="ACIYSB_004651"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGC5431538.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLQRETVDFNGKHIHVRGA
                     KLLFPAIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPLLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2857..3648
                     /gene="ssuC"
                     /locus_tag="ACIYSB_004652"
     CDS             2857..3648
                     /gene="ssuC"
                     /locus_tag="ACIYSB_004652"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGC5431539.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3645..4412
                     /gene="ssuB"
                     /locus_tag="ACIYSB_004653"
     CDS             3645..4412
                     /gene="ssuB"
                     /locus_tag="ACIYSB_004653"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGC5431540.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4455..7067)
                     /gene="pepN"
                     /locus_tag="ACIYSB_004654"
     CDS             complement(4455..7067)
                     /gene="pepN"
                     /locus_tag="ACIYSB_004654"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGC5431541.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRGWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     ADDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7329..8531
                     /gene="pncB"
                     /locus_tag="ACIYSB_004655"
     CDS             7329..8531
                     /gene="pncB"
                     /locus_tag="ACIYSB_004655"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGC5431542.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8700..10100
                     /gene="asnS"
                     /locus_tag="ACIYSB_004656"
     CDS             8700..10100
                     /gene="asnS"
                     /locus_tag="ACIYSB_004656"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGC5431543.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2321 a         2643 c         2775 g         2549 t
ORIGIN      
        1 acatctaatg actatccaat aaataaatat gcgtgatata taaagccctt tctttagttt
       61 attttcagaa aaagatacac aaaatttata tttggaattt tcttgtctct ccgacagact
      121 gaccgaattg ttatcaatgt taacaaaaaa agaacaattg gttataagga gagagtatgc
      181 gtgtcatcac cctggcgggt agtcctcgct ttccttctcg ctccagctcc ttgctggaat
      241 atgcgcggga aaaactaaat ggcctggatg tagaggttta tcactggaat ctgcaaaact
      301 tcgccccgga agatctactt tatgctcgtt tcgatagtcc ggcactcaag accttcaccg
      361 aacagctgca acaggccgat gggctgattg tcgccacgcc tgtgtataaa gccgcctatt
      421 ccggtgcgtt gaaaaccctg ctcgacctgc tgccagaacg cgctttgcaa ggcaaagtgg
      481 tgctaccgct ggcgacgggc ggtaccgtgg cccatctgct ggcggtcgat tatgccctta
      541 aaccagtttt aagcgcactg aaagctcagg agatcctgca cggcgtgttt gccgatgact
      601 cacaagtaat tgattaccat cacagacccc agttcacgcc aaatctgcaa acccgtcttg
      661 ataccgcgct agaaactttc tggcaggcat tgcaccgccg cgatgttcag gttcctgacc
      721 ttctgtctct gcgaggtaat gcccatgcgt aacatcatta aactggcgct ggcgggattg
      781 cttagcgtct ctacttttgc ggttgctgca gaatcctcgc ctgaagcgtt acgtataggc
      841 tatcagaaag gcagtattgg tatggtgctg gcaaaaagcc accagttact ggaaaaacgc
      901 tatccggaat caaaaatctc ctgggtggag ttccccgcgg gtccgcaaat gttggaagcg
      961 ttaaacgttg gcagtattga tctcggcagt accggggata ttccgccaat ctttgcccag
     1021 gctgccgggg ctgatttggt gtacgtgggc gtcgagccac cgaagcccaa agccgaagtg
     1081 attctggtgg cagaaaacag cccgatcaaa accgtagccg atcttaaagg tcacaaagtt
     1141 gcctttcaga aaggttccag ttcacacaac cttttactgc gtgcactgcg tcaggccgga
     1201 cttaagttta ccgatatcca acccacttac ctgacgcccg ctgatgcccg cgccgcgttc
     1261 cagcaaggta acgttgacgc ctgggctatc tgggatccct actactctgc tgcattatta
     1321 cagggcggcg tgcgggtgct gaaagacggc accgatctca atcaaactgg atcgttttat
     1381 ctggcagctc gtccctatgc agaaaaaaac ggcgctttta ttcagggcgt actggcaacc
     1441 tttagtgagg ccgatgcgtt aacccgcagc cagcgcgagc aaagcatcgc tttactggca
     1501 aaaacgatgg gcttaccggc accggtgatt gcctcttact tagatcatcg ccctcctacc
     1561 accatcaaac cggttaacgc cgaggttgcc gccttacagc agcaaacggc agatctgttt
     1621 tatgaaaatc gtctggtgcc gaaaaaagtc gatattcgcc agcgcatctg gcagcccact
     1681 caactggaag gaaaacaatt atgagtctga atatgttctg gtttttaccg acccacggtg
     1741 acgggcatta tctgggaacg gaagaaggtt cacgcccggt tgatcacggt tatctgcaac
     1801 aaattgcgca agcggcggat cgtcttggct ataccggtgt gctaattcca acggggcgct
     1861 cctgcgaaga tgcgtggctg gttgccgcat cgatgatccc ggtgacgcag cggctgaagt
     1921 ttcttgtcgc cctgcgtccc agcgtaacct cacctaccgt tgccgcccgc caggccgcca
     1981 cgcttgaccg tctctcaaat ggacgtgcgt tgtttaacct ggtcacaggc agcgatccac
     2041 aagagctggc aggcgacgga gtgttccttg atcatagcga gcgctacgaa gcctcggcgg
     2101 aatttaccca ggtctggcgg cgtttattgc agagagaaac cgtcgatttc aacggtaaac
     2161 atattcatgt gcgcggagca aaactgctct tcccggcgat tcaacagccg tatccgccac
     2221 tttactttgg cggatcgtca gatgtcgccc aggagctggc ggcagaacag gttgatctct
     2281 acctcacctg gggcgaaccg ccggaactgg ttaaagagaa aatcgaacaa gtgcgggcga
     2341 aagctgccgc gcatggacgc aaaattcgtt tcggtattcg tctgcatgtg attgttcgtg
     2401 aaactaacga cgaagcgtgg caggccgccg agcggttaat ctcgcatctt gatgatgaaa
     2461 ctatcgccaa agcacaggcc gcattcgccc ggacggattc cgtagggcaa cagcgaatgg
     2521 cggcgttaca taacggcaag cgcgacaatc tggagatcag ccccaattta tgggcgggcg
     2581 ttggcttagt gcgcggcggt gccgggacgg cgctggtggg cgatggtcct acggtcgctg
     2641 cgcgaatcaa cgaatatgcc gcgcttggca tcgacagttt tgtgctttcg ggctatccgc
     2701 atctggaaga agcgtatcgg gttggcgagt tgctgttccc gcttctggat gtcgccatcc
     2761 cggaaattcc ccagccgcag ccgctgaatc cgcaaggcga agcggtggcg aatgatttta
     2821 tcccccgtaa agtcgcgcaa agctaaggaa gaaataatgg caacgccagt gaagaagtgg
     2881 ttattgcgcg ttgccccctg gtttttaccg gtgggcatcg tggcggtgtg gcaactggcc
     2941 tcctcggttg gctggctgtc gacgcgtatt ttgccttcac cggaaggagt ggtgacggcg
     3001 ttctggacgc tctccgccag cggcgaactg tggcagcatc tggcgatcag ctcctggcgg
     3061 gcgctgattg gcttttcaat tggcggatcg ctggggttga ttttgggact gattagcggg
     3121 ctatctcgct ggggagaacg gctgctggat acctcaattc agatgttgcg caacgtgccg
     3181 catctggcgc tgatcccgct ggtgattttg tggtttggca ttgatgaatc cgcaaaaatc
     3241 tttctggtgg cgctcggtac gctattcccc atttatatca acacctggca tggcatccgt
     3301 aatatcgatc gcgggttggt ggagatggcg cgtagctatg gattatccgg cataccgctg
     3361 tttatccatg tgatcctgcc tggtgccctg ccctcaatta tggtcggcgt gcgttttgcg
     3421 ttgggcctga tgtggctgac gctgattgtt gccgaaacca tttctgccaa ttcaggcatt
     3481 ggttatctgg cgatgaatgc gcgggagttt ttgcaaacgg acgtggtggt agtcgctatt
     3541 attctttacg ccctgctcgg caaactggct gacgtcagcg cgcagctgtt agagcgcctc
     3601 tggttgcgct ggaacccggc ttatcatttg aaggaggcca cggtatgaat actgctcgtc
     3661 tgaaccaggg cacgccattg ttgctcaatg cagtaagcaa acattacgcg gaaaatatcg
     3721 tcctgaacca actggattta catattccgg caggtcagtt tgtggcggtg gtgggccgca
     3781 gcggtggtgg caaaagtacc ctgctgcgcc tgctggcagg tctggaaacg ccaaccgcag
     3841 gcgatgtgtt agcgggcacc acaccgctgg ctgaaattca ggaagacacg cgaatgatgt
     3901 ttcaggatgc gcgtctgcta ccgtggaaat cggtgattga taacgttggg ttaggcctta
     3961 aaggccagtg gcgcgatgcc gcacgtcgag cgttggctgc ggtaggactg gagaatcgcg
     4021 caggggaatg gcctgccgca ctttctggcg ggcagaaaca gcgagtggcg ctggcaagag
     4081 cgttgattca tcgaccggga ttattgttgc ttgatgaacc gctcggggcg ctggacgcct
     4141 taacgcgact cgagatgcag gatttgattg tgtcactttg gcaggagcac ggctttaccg
     4201 tactgttggt gacgcatgat gtgagcgaag cggtagcgat ggctgaccgg gtgctgttaa
     4261 ttgaagaggg aaaaattggt ctggatttga cagtggatat cccccgtccg cgtcgattag
     4321 ggtcggtgcg attagcggag ctggaagcgg aggtgttgca acgggtgatg caacgtggtg
     4381 aatctgaaac tcgcctgaga aaacaggggt aaattccccg aatggcggcg ctattgccgc
     4441 cattcggtta tttatcaagc cagtgcttta gttatcttct cgtacagatc gccagagaga
     4501 ttttccagcc ctttcaactg ctccagcgcc gcgcgcattt tctcctgacg tttggcatcg
     4561 taacgtttca ggcgaatcag cggttcaatc agacgtgaag ccacctgcgg gttacggctg
     4621 ttgaggtcgg taagcatttc caccaggaac tggtaaccac tgccatcgtc ggcatggaac
     4681 gctgccgggt tgctgcccgc aaacgcgcca atcaacgaac gaatacggtt cgggttgctc
     4741 atggtaaatg agcgatgctg caacagacca cgtaccgtct ccagcacatt cgccgccggg
     4801 ctggtggctt gcaggataaa ccatttatcc atcaccagac catcctgatg ccacttgtcg
     4861 tcgtactcct gcatcagcgc gtcacggcat ggcagctgtg cggcaaccgc cgcagaaagc
     4921 gccgccagcg catcggtcat attgttcgct tcatggaact gcttgctcac cagcacatca
     4981 gccagatgcg tttcaccaaa agcaaggaag cgcagacagg cattacgcag agtgcgtttt
     5041 gcaatatctt catgctcaac gcggtactcg ctctggtaat tcgcgttgta aatagccagc
     5101 agctcatccg ccagttcagt cgccagagta cgagtgagtg cttcgcgtac ttcggcaata
     5161 gcaatcgggt cgatgatatc gaacagttca gccatttcat tgacggatgg cagagtcaga
     5221 atttctgccg ccagtgccgg gtcaatcttc tcatcgagca gcaccgcgcg gaaggcgtcg
     5281 gccacatgca ccggcagaga cagcggctgc ccttgctgat gacgggcgac gttcagcttg
     5341 atgtaggttg ccagcaaact ttgcgccgca tcccaacggg agaaatcatt acgcgcatga
     5401 cgcatcagga aggtcagttg ctgatcgctc cacttatatt ccagtttcac tggcgcagag
     5461 aattcgcaca gcagcgcagg caccggctgg aagtagacat tatcaaagac aaaggtctgt
     5521 tccgcctgag tgacgttcag cacggaattc accggatgac cgcctttctg caacgggatc
     5581 actttgcctt cgttatcata cagttcgatg gcaaacggaa tatgcagcgg ctgtttttct
     5641 gcctgatccg gcgtggctgg cgtgcgctgg ctgatggtca gggtgtactg ctcggtttcc
     5701 ggattgtagt cgtctttgac ggtcacaatc ggtgtaccgg actggctgta ccaaccgcgg
     5761 aaatgggaga gatcgacatt cgacgcatct tccatcgcct gcacaaagtc gtcacaggtc
     5821 gctgcactac catcatgacg ctcgaaataa agctgcatcc ctttctggaa gttttcttcg
     5881 ccaagcaggg tgtggatcat gcgaatcact tccgcgccct tctcgtaaac ggtcagggtg
     5941 tagaagttgt tcatctcaat gaccatatcc gggcggatcg ggtgcgccat cgggctggcg
     6001 tcttctgcaa actgcaatcc gcgcatggtg cgtacattat tgatgcggtt aactgcgcgg
     6061 gaaccaaggt cagagctgaa ctcctgatcg cggaagacgg ttaaaccttc tttcaggctg
     6121 agctggaacc agtcgcgaca ggtcactcgg ttaccggtcc agttatggaa atattcatgg
     6181 ccgataacgc gttcaatatc gaggtaatct ttgtcggtgg cggtgtcggt gcgggccagc
     6241 acatatttgg agttaaagat attcagcccc ttattctcca ttgcgcccat attgaagaaa
     6301 tccaccgcga cgatcatata gatgtcgagg tcatactcca ggccaaagcg ttcttcatcc
     6361 catttcatgg agtttttcag cgaggtcatc gcccacggcg cgcgatcaag gttgccgcga
     6421 tcgacgtaca gctccagtgc tacttcgcga ccagaacgcg tggtaaaggt atcgcgcagt
     6481 acatcaaagt cgcctgccac cagcgcaaac aggtagcacg gtttcgggaa cgggtcctgc
     6541 cactgtaccc aatggcgtcc gttttccagt tcgccttgcg caacgcggtt accattggaa
     6601 agcaggaagg gatatttgat tttatcggca ataattttgg tggtaaaacg cgccagcacg
     6661 tccgggcggt cgagataata cgtaatatgg cggaaacctt cggcttcaca ctgggtgcaa
     6721 agcgcatcgc ctgactgata aagcccttcc agcgcggtat tcgccgccgg gcttatttca
     6781 ttaatgatct taagcgtaaa acgctccggc aaattactga taaccagtgc gccctcttct
     6841 tctttccagg cggtccacgg ctcatcatta atatgaacag aaaccagttt gaggtcttcg
     6901 ccgttgagac gaaggggagc atctgatgca ccatgacgga cagcctggct gaccgcggta
     6961 acgaccgtct tttgcgcgtc gaggtcaaag gtcaagtcaa tatcagtaat ctggtaatcc
     7021 ggcgcacgat aatcgtgacg gtatttggct tgtggctgtt gagtcataaa taacctttag
     7081 catcttttat agagtctggt gttcagtcta ttcctgttgc gtaaatcgcg ctatgcagaa
     7141 tcttcatctt ttcaggtaca aacgccttta ttgctacatt tttataacat acaccgcgta
     7201 atgcgaccag aaaggtggca tatggtgtga tcggggttca ataaattgcg aaacaaggta
     7261 tactccagca gttcctgaag atgtttattg tactaaacgc tcctgtacga ggacgctact
     7321 gcgcacctat gacacaattc gcttctcctg ttctgcactc gttgctggat acagatgctt
     7381 ataagttgca tatgcagcaa gccgtgtttc atcactatta cgatgtgcat gtcgcggcgg
     7441 agtttcgttg ccgaggtgac gatctgctgg gtatttatgc cgatgctatt cgtgaacagg
     7501 ttcaggcgat gcagcacctg cgcctgcagg atgatgaata tcagtggctt tctgccctgc
     7561 ctttctttaa ggccgactat cttaactggt tacgcgagtt ccgctttaac ccggaacaag
     7621 tcaccgtgtc caacgataat ggcaagctgg atattcgttt aagcggcccg tggcgtgaag
     7681 tcatcctctg ggaagttcct ttgctggcgg ttatcagtga aatggtacat cgctatcgct
     7741 caccgcaggc cgacgttgcg caagccctcg acacgctgga aagcaaatta gtcgacttct
     7801 cggcgttaac cgccggtctt gatatgtcgc gcttccatct gatggatttt ggcacccgtc
     7861 gccgtttttc tcgcgaagta caagaaacca tcgttaagcg tctgcaacag gaatcctggt
     7921 ttgtgggcac cagcaactac gatctggcgc gtcggctttc cctcacgccg atgggaacac
     7981 aggcacacga atggttccag gcacatcagc aaatcagccc ggatctagcc aacagccagc
     8041 gagctgcact tgctgcctgg ctggaagagt atcccgacca acttggcatt gcattaaccg
     8101 actgcatcac tatggatgct ttcctgcgtg atttcggtgt cgagttcgct agtcggtatc
     8161 agggcctgcg tcatgactct ggcgacccgg ttgaatgggg tgaaaaagcc attgcacatt
     8221 atgaaaagct gggaattgat ccacagagta aaacgctggt tttctctgac aatctggatt
     8281 tacgcaaagc ggttgagcta taccgccact tctcttcccg cgtgcaatta agttttggta
     8341 ttgggactcg cctgacctgc gatatccccc aggtaaaacc cctgaatatt gtgattaagt
     8401 tggtagagtg taacggtaaa ccggtggcga aactttctga cagccctggc aaaactatct
     8461 gccacgataa agcgtttgtt cgggcgctgc gcaaagcgtt cgaccttccg catattaaaa
     8521 aagccagtta atatcatcag ggagctaatc ggctcccttt ttttaccttt aattccgaaa
     8581 tctttcgctg catttgcgaa ttctgcttgt ctgattgcag atgccaggta acataggtat
     8641 ccccccattt acgggtggac aagtgtttat tttttccgac tattaacaga gagaatatta
     8701 tgagcgttgt gcctgtagcc gacgtactcc agggccgcgt agccgttgac agcgaagtca
     8761 ccgtacgcgg atgggtacgt acccgccgag attcaaaagc tggcatctcc ttcctcgccg
     8821 tttatgacgg ttcctgcttt gatcctgtac aggctgtcat caataattct ctgcccaatt
     8881 acaatgaaga cgtcctgcgt ctgaccaccg gttgctcggt cattgtgacg ggtaaagtcg
     8941 tggcgtcgcc gggccagggg caacaatttg aaattcaggc cagcaaggtt gaagttgctg
     9001 gttgggttga agatccagac acttacccga tggcggcaaa acgccacagc attgagtatc
     9061 tgcgtgaagt cgctcacctg cgtccgcgca caaacctgat tggtgccgtc gcgcgcgttc
     9121 gccatacgct ggcgcaggcg ctgcatcgct tctttaacga gcagggattc ttctgggttt
     9181 caacgccact gattactgcg tctgataccg aaggggctgg cgaaatgttc cgcgtttcta
     9241 cgctggatct ggaaaatctg ccgcgtaacg atcagggcaa agtggatttc gacaaagact
     9301 tctttggtaa agagtctttc ctgaccgtat ctggccagtt gaacggcgaa acctacgctt
     9361 gcgcattatc caaaatttat accttcggcc cgactttccg tgctgaaaac tccaacacca
     9421 gccgtcacct ggcggagttc tggatgctgg agccggaagt ggcgtttgct aacctgaacg
     9481 atatcgcggg tctggctgaa gccatgctga aatatgtctt caaagcggtt ctcgaagaac
     9541 gcgcagacga catgaaattc ttcgctgaac gcgtagataa agatgccgtt tcacgtctgg
     9601 aacgcttcat tgaggccgat ttcgcgcagg tggattacac cgacgcagtg accattctcg
     9661 aaaactgcgg caggaagttt gaaaacccgg tttactgggg cgtcgatctc tcttctgagc
     9721 atgagcgtta tctggcggaa gaacacttta aagcaccggt agtggttaaa aactatccga
     9781 aagatattaa agcgttctat atgcgcctca acgaagacgg taaaaccgtt gcggctatgg
     9841 acgttctggc tccgggcatc ggtgagatca ttggtggctc ccagcgtgaa gaacgtctgg
     9901 acgttctgga cgagcgtatg ctggaaatgg gcctgaataa agaagattac tggtggtatc
     9961 gcgatctgcg tcgctacggt actgttccgc attcaggttt cggtcttggt tttgaacgcc
    10021 tgattgctta cgtaactggc gtgcaaaacg tgcgtgatgt gattccgttc ccacgtactc
    10081 cgcgcaacgc cagcttctga ttttcttcct caaagagcca gcgcctgctg gctctttttt
    10141 atcctttctg tcaagttatc tgtttgttaa gtcaagcaat ctatttgcaa ccccgccata
    10201 acgatcatcc tgttatggaa tattacattg caacatttac gcacaaaaaa taacctggat
    10261 tcttattgcg gattagtttt ttcttagc
//
LOCUS       DBBGLQ010000136        10165 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.136, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000136 DBBGLQ010000000
VERSION     DBBGLQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.136"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            2..259
                     /locus_tag="ACIYSB_004657"
     CDS             2..259
                     /locus_tag="ACIYSB_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431544.1"
                     /translation="MQGASLGVLRKDASARTRDKEGIVFVRTGREAGVLIIAELFSEV
                     VTVSQPVPGEQETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            complement(977..1177)
                     /locus_tag="ACIYSB_004658"
     CDS             complement(977..1177)
                     /locus_tag="ACIYSB_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431545.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            1377..1946
                     /locus_tag="ACIYSB_004659"
     CDS             1377..1946
                     /locus_tag="ACIYSB_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGC5431546.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            2206..2607
                     /locus_tag="ACIYSB_004660"
     CDS             2206..2607
                     /locus_tag="ACIYSB_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGC5431547.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            2595..3005
                     /locus_tag="ACIYSB_004661"
     CDS             2595..3005
                     /locus_tag="ACIYSB_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HGC5431548.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            3289..4908
                     /locus_tag="ACIYSB_004662"
                     /pseudo
     CDS             3289..4908
                     /locus_tag="ACIYSB_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            4988..5280
                     /locus_tag="ACIYSB_004663"
                     /pseudo
     CDS             4988..5280
                     /locus_tag="ACIYSB_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(5320..6354)
                     /locus_tag="ACIYSB_004664"
     CDS             complement(5320..6354)
                     /locus_tag="ACIYSB_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HGC5431549.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(6357..7322)
                     /locus_tag="ACIYSB_004665"
                     /pseudo
     CDS             complement(6357..7322)
                     /locus_tag="ACIYSB_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(7379..8137)
                     /locus_tag="ACIYSB_004666"
     CDS             complement(7379..8137)
                     /locus_tag="ACIYSB_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HGC5431550.1"
                     /translation="MNNTKKSLKVLFIGESLHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNAPLLVFSNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(8151..9365)
                     /locus_tag="ACIYSB_004667"
     CDS             complement(8151..9365)
                     /locus_tag="ACIYSB_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HGC5431551.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTETATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGSLASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(9561..9894)
                     /locus_tag="ACIYSB_004668"
                     /pseudo
     CDS             complement(9561..9894)
                     /locus_tag="ACIYSB_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2680 a         2366 c         2512 g         2607 t
ORIGIN      
        1 gattcagggg gccagtctag gggtgttgcg aaaagatgct tccgcacgca cgcgcgataa
       61 agaggggata gttttcgtga ggacagggag agaagcaggc gttctgataa ttgcagagtt
      121 gttttcggag gtggttacag tgagtcagcc cgttcctggc gagcaggaga ctgatatcac
      181 cgatgggata aggttgtctg attggtatta taaatataag aagtcatcag agagtaacgt
      241 aaaggatttg ccagaataac ctaagagagt aaatctctcc ggtaacagag cgctgtgaag
      301 cattctgtat cagagtgcag atacatgaac gctgatgcgt aaaatcagca gtgacacgcc
      361 agctccccct atgcccattc ctgtataaga catgaactga gtgtattaat gcttctgccc
      421 tgagactgag ccggtgagtg aggggctgag aaatccctct tgacacagct ctaaccagaa
      481 atacttcccc ggttgttgcg gtgcgtccgg atccccgata acggaacact aaggctgtat
      541 tgcgggacac cgggtaagta gcagcccgta aggcgttctg tcaacgccag aaaaaatgat
      601 ttcccacacg cgttatagac tcactcctgt ggacctggaa gcccgtaact ctgcggttgc
      661 ccactgactg acggaactga ccgcaaattt tacgagtgcg tgctgggctt cagtctggtc
      721 acctgagcgt tcggtaacgg aggcgcgggc ggtaataggc tgattttcag gtattaccgg
      781 cttacagcgt gtggttgata ttgaggggct gtgagtttcg cgtatttgtc agaaacccag
      841 acgagttttc ctgtatggga cgaaataagg cagcggcaat acctggaaaa atttaatatg
      901 gtatgaagga gaacctgtcg gttacgctta atatacccgg tacactgtca gctcagggga
      961 tacatgaacc gttatctcac cagctacaca gtatatacac actgacgctg acagtagtca
     1021 gcaggaccac aataatcagt gccagcagta tggtaacgat acggtctgta ttccggtaat
     1081 agccgaaggg gtcgacagaa ccacaatacg ggcaacacac tgtattatga gtaatcgcgt
     1141 ttccacagtc cctgcacttc tttttgcgta tcaccatcac tccctcctca cagccttacc
     1201 cgtaaccggt tttttacatt aaaaaaacaa cgctgaaaat tcaaccgtca gtccggagac
     1261 gaccgttcgg gttatcacag agtgcctgag acagtgtcct gccggaggtc acttcaggga
     1321 ctctgcgcat tttttacgac gtggctattc cgtatgaacc atacggagat ttaaccatga
     1381 cctacaaata caaccccttc tggcagcaac gtattcgtga gacggtgcgg cacgcactga
     1441 atgttcatcc ccgcctgacg gcattgcggg ttgacctgcg tttcccggat gtaccggcag
     1501 caacggacgc agctgtgata tcccgcttca tcaatgccct gaaagcccga atcgacgctt
     1561 accagaaacg taagcatcgg gaaggtaaac gcgtgcatcc caccaccctg cattacgtct
     1621 gggcccggga gtttggggag tgcaaaggta aaaaacacta tcacctgatg ctgctggtta
     1681 accgggatac ctggtgtcgt gccggtgatt accgtgctcc gggatcactg gccgggatga
     1741 ttaaacaggc gtggtgcagt gccctgggag tggatgtcgg gtgccatgcc acgctggtgc
     1801 attttccggc ctggccggcg gtgtggctgg aacgcgatga tgacactggc tttcagcagg
     1861 tgctggaacg tgctgactat ctggcgaagg aacataccaa agctcactgc accggtgagc
     1921 gcaattttgg ctgtagccgg agttgagcac gactggcgcg ccaatatccg gcaaaccaaa
     1981 agtatgggtt gttgtctggc ggcaacgatg tatccactcg cccactgact gttttttacg
     2041 tcagtgttcc attgtgtgac aaccacagtg ctgtaacagc aaccagcctg ctttacccaa
     2101 actccagtcc agattttaac tgaaccgcca ttcacaccct gaccacgctg cccgtaccgg
     2161 gacggctgtt gcctgcgtgt cgtctggcgg ctgaggaata tgactatgta cgcaaaatcc
     2221 tttatcgctc ttgatggcaa cggacgtctg acgggcgccc gtactgcaca ggccgcacct
     2281 tatgctaact acacctgcca cttgtgtggc agtgcactca gataccatcc gcaatacgac
     2341 actgaacttc cctggtttga acacactgac gacaggctga cagagcacgg tcaacagtgc
     2401 ccttatgtca ggccggagcg cagagaaata cagttgatta aacgtctgca gcaattcgta
     2461 ccggatgcct tacccgtggt gcgtaaagcc agctggcact gcagacaatg tcaccacgat
     2521 tattatgggg agcggtactg cacacactgc cagaccggag gattcagtat tccccggaca
     2581 actcaggagg aaatatgcga attctgaact gttatatggc gaatgacagc aaaggccatt
     2641 ttgttacggc gaaagaagca gcgaagcaca accgccagga cgttttgtgc tgtgtgtcct
     2701 gtggatgccc gttaacactt cagcggggca atgacggaca accaccgtgg tttgaacatg
     2761 accagatgac tgtcgctgag aaaatcctgc tgcgatgcac ctggcttgac ccggcagaga
     2821 aagaggcccg tcgtttgcat ctgcagggta tgacggttcc ggattatacg gtgaaggtga
     2881 gaaagtggtt ttgtgtgatg tgtgacgaag attatgaagg agaaaagtgc tgcccgcgtt
     2941 gtggtaccgg ggtatacagc agggaggggg ggctgcagga aggcaactgg aaggatagga
     3001 actgagcggt tcagtcgcgg aaatcagttg cagagctgta atgatatctc cgggagtgct
     3061 gcgttggtag gggcatcccg ggatagagaa attttagcac tttgccctga atgattgtag
     3121 acgttccgag tactcgtacc ggtctgagtt attacagtta gatattcact ttttctcccg
     3181 catacttcac atctgtcaac gacggatgaa aagtggttct tccgcaacct gggtggaaga
     3241 aaatggatga aactttctgg cgaacaagga tctaagctgt tttaagttat gggcaacgca
     3301 atgcactctc ttaagacact tctacagtta ccttgcggat ggcgatgcag tcgacaaatt
     3361 attagctctg acggtataac cctccatctc cacggaaaac gcaaaacagc acaatgtcct
     3421 gaatgcttta agcgtagcga ctctgttcat agttgtcgtc ggcgccggat acagcatcta
     3481 ccctgctcag ggcagacgct atggcttgta ttttccgtcc gccactggta ctgccgtaac
     3541 ccggcttgtt cacgtaaaat ttttgccgag tcgcttgctc ccttcgccgg ttcacaccag
     3601 cagtcttcac aggcgttaca aaatttacaa cgtcaactgg gattaatagc tggaggtgag
     3661 gctggaaaac gggctgcaac ggcagcgggt ctccgttgca gtgctgatac tcttcttcgc
     3721 agggttatca ataccccgga gacgaaacag tcaggcgcgc ctcatgtcgg tattgatgag
     3781 tgggcgtggc atcggggcca ctgctgcggt atgttaatag tcaatcttga tactcaccgt
     3841 cccctcgtcc tgcttcccgg ccgtgatcag cgtacgctgg cgacctggtt cagaaaatat
     3901 ccggaaatac aggttgtctc gcgtgatcgc agtggagtct atgcgacagc agcacgtgaa
     3961 ggtgcacctc aggccagaca ggtggccgat cgatggcacc tgctaaaaaa tattggtgat
     4021 gagcctgaac gaatgatgta cagacatatg cctctgatac gtcttgttgt cagagagtta
     4081 tcactgaaga aatcacctga gccagaaata tctgtgcctg tagcatcgct ccgtcgtctg
     4141 gaacgcctta aacagcacat ccgcaaaaaa cggcatcagc gttggacaga ggttatggcc
     4201 ctgcataaca agggatgtag tttcagggaa atatcccgta ttacaggcct gtcgcgagtg
     4261 acagtcagtc gctgggtggg ttcaggaaca ttccctgaaa tgtcaactag gcctccaaag
     4321 cgagggcttc tggacccatg gagggagtgg ttaaaagagc aacgagaatg tggtaattat
     4381 aactccggcc ggatatggcg ggaaatggtg gccagggggg ttacaggcag tgaaaccatc
     4441 gtcagggatg ctgttgccaa atggcgtaaa ggctggatcc caccggttac tactgccgca
     4501 agacttcctt cagtgtcccg ggtaagccgc tggttgatgc cctggagaat aatcaggggt
     4561 gaagaaaatt atgctttccg atttattagt ctgatgtgtg aaaaagaacc ggagttgaaa
     4621 atagcgcagc aactggtact cgagttctac cgtattctga aaacctaaaa taaatcacag
     4681 ctaagcagct ggttcactcg ggtccacgaa agcggctcag cagaacttcg gtgcgtagct
     4741 gcggggatgg aagctgatgc tgcggctata tgtgaggcaa tcagcagtcg ctggagtact
     4801 ggtgttgtcg aaggtcatgt aaatcgcctg aaggtgctga tacgccagat gtatggtcga
     4861 gccggacttg aactacttag gcggagggta atgagtccac tggcatgaaa atcttcaccc
     4921 aggttgcgga agagccactt ttgcactgtt cgtgacagta tggcctttat gtactcactt
     4981 tgttaccggt agctggatca tcactaccgt gctgcaaatg gtggtgaaca gcatcaattg
     5041 tggaatgaat gttgccgaag cgaccaatgc gcctcgtttc cactatcagt ggttgccgga
     5101 cgagctgcgt gttgaaaagg gtttagtccg gatacgctca agctgctgga tgcaaaaggt
     5161 cagaaagtgg cgctgaatga ggcgatgggc agcacgcaaa gcattatggt tgggtcggat
     5221 ggtgagttgt acggcgcatc cgactcgcgc ttggtggatg atttaacggc ggggtattaa
     5281 ttgacatccc ccggcgcaat gtgccggggg ataatggttt tatgcggtca ataatcgtcc
     5341 gggattatcg atacagagtt ttttgaggat cgttttatcg acgccgcgct ccgccagata
     5401 ggccagaaaa acatctggaa caaatcccca gccattaccg ccatttttag cccacatttg
     5461 tttaaggaag acatcgtggc tgagaacaag ctgatcggcg tatcccaact caatgagatg
     5521 agcgacggca tcggcagtct cctgcacccc tggcgctatt ccctctttcg ggaaggtaat
     5581 gtctaggcca atcatgtcga attccagcca gacaccttta tcaagcattt tccgctgata
     5641 cgccacgtct tttcctgacg gatccgagtg tgcgagagag actttatttg gcgagacgcc
     5701 catttcacct aacacaatgt cgagtacttc atcacctcga cgaagccagc ccggcatgtg
     5761 aatattcatc gccacatgag gattgttaat ctgtgccagc gaggcagccc gcaagctgtt
     5821 atgctcggct tcggtaaatg tcggtgaaac acctatttca ccgatcattc cggcacgaat
     5881 atccgtatcg ccaatccctt gattcaattc tttatcgatc gttgtcgcca gttcatcaac
     5941 cgttttatga attctctggc tttcaaattt ttccaggtag ggaccggagg aagcaacaat
     6001 attcagacct gtttttaatg cgacttctcg taatgcctgc gcatctcgtc cgatagactc
     6061 agaccctgtg gcatcaacaa tggtgcgacc accgagcgag ataaagttat taatttcgaa
     6121 gatcacatct tcaataggtt tgcggtccat attatcagcg cagcaataag gatcgtgttt
     6181 taatgcccac tggatttctg ccgtgacttt tttatcaaca agccgttgtg aaaaaggata
     6241 gcaaggagca tccacaacag aggaaagatc gttgaacaga tgttcatgag gaagtgttag
     6301 ccccatatct tcacgggcga cggggcccgt cacagtttgt agataatctt tcatgattat
     6361 acctccattt tcttcattgt tttcgaggtt ctgtattccg gaatagtggt aacaattgca
     6421 ccgaccagtg caaacagtgt tccgatgatc gtgaccagat aaacgctatt gcccagcgat
     6481 ggaattaata cgtcaatcaa tactgatccc agtaactggc ccgccgttga agcaaccccc
     6541 agcatcagta accccagacc tctgaccaaa atggccatta aaccgataga cattagtcca
     6601 agcgggccgc caagatacat ccaccagaca tctggtaatt gaacagtgac gtgccctaat
     6661 gcgacacgaa tagctagcgc tgtgcctaaa acacaaaatc caacgatgaa attctaggtg
     6721 attgaaacca gcatggatcc cgtagcttca gctacttttg catttccagc gggctgccat
     6781 cctgcaagca aaccagctaa gaacggaaga atggcaagta agatgaaaga ggtagaatgc
     6841 cactgaggtg acacaacaaa aatcgtagca ataaccgcaa ataatgcacc gatgatgcga
     6901 taaggggtaa agtatttttt ctcgtcgaca ccgataccaa agcgatcgca aagtaaacca
     6961 gacagcaata gcgcggagat cagtgcggtt tgaaacgttg cgaccccgag tgcgctggct
     7021 gatgcgcctt cagaaaatac caccatcgct ccacagagac cggcaaacca gttccagaga
     7081 ggaacttttc ggtttttaat cagagaagga attgccgcaa attgttgccg ggtttctttt
     7141 cgcgccataa taataaaaaa catcacgact aaaccactgg caaaggagat aactgcgcat
     7201 gcattaccat cctgtaacca gtgacctaat tgaccattca ccgctgattg cataggggag
     7261 agcatacccg caagaatcgt ggcaagcatc cataagggag ttgagtactt attctttttc
     7321 atacatcaat ccttctatta tagtaaatgg agtaactgga gttaccccat tattattatt
     7381 atcttgcaat atgagtcagt atatttaccc acaacgcggt atagaacggc caggacataa
     7441 actgctgtgt accccagtgc ggtgaacagt cactcataaa gcaggctatt ttcccgttgt
     7501 gatagttact aaagaccagt aatggagcgt tgtttattgt taataccacc tcagcatttt
     7561 ctttagcgat cgcccggtta taacctagga aaaaaggata ttcagagaac cctttaataa
     7621 ctggatgttc aggttgtgat ggctgtgcaa taacaccctc tggtttttcg acacgatcgt
     7681 caccatctaa catcgtaacc gggagcacat cagccagaac tgtattttta taattggctt
     7741 ttgcttcaat tcccatgaat gagagatagc cgccaatcat caataaccca ccaccgttat
     7801 tcacatattc tttaatcagt tcgagagcgt ttggttttat gcgaagctga taaaaagtat
     7861 cattttgtag caggaaagta ttactaccaa tatcactaat aacgatggca tcatattgtt
     7921 ctaactgtgc cacatcttca gggaaggcaa tttgtaccgt atgtgctggc atataggtga
     7981 catctacctg actatttttc agacactgga gtaaccaggt cgcaccttct tcatatttac
     8041 ttgaggtaaa actgtcatat ccttttgaat gaatcatatg aatatgcaag gattccccaa
     8101 taaagagcac ttttagtgat tttttggtgt tattcattct ctgtcccttc ttaatgcgta
     8161 aaaatagttt gctgataatg tatactctga attcgatgta tcacagattc atgttcaggc
     8221 atgtcggaag cattgcttgt ctccaccgct agggatgcaa aggcgctggc ataacacagt
     8281 gctgattcca gtgattttcc tttcgcaagt cctgatgcca gagagccgtt aaacgcatcc
     8341 ccggctcctg ccgtattttt aacgaccgct ggaaacgctg gcgaatgaat gaattttttg
     8401 ccatcaaatg cgagcgagcc cttgctaccg agcgtgataa ccgtgttttt taccccttta
     8461 ttgtggatag cttctgcagc tcgtttagct gattcaagat cgtgtacgtc tatgccggac
     8521 agtaaaccag cttctgtttc attgggggtg atataatcaa catcctgaat aagttcgttg
     8581 acgatatcat tatagggtgc cgggttaata atgaccggga tactgttttt ttgcgccaat
     8641 gtgatcgctt gttgcagagc ggtataattt gtttcgagct gcagaagtat gacatcagaa
     8701 ttaataatcg cttctttctg aatagttatt tcatcggacg acattgtcat gttggcaccg
     8761 ggatagatcg caataacatt atcgccagta ccttcattaa caaggatcgt ggcagtttcg
     8821 gtttgcgttt ctttcgtttg ataaattata cttttataaa tcttagacga attcataaag
     8881 ttgatcgcat aatcactaaa gtgatctgta cctacttttg ttatgaaata aacatcagta
     8941 tcggcaaagc ttgctgccag cgcctggtta cagccttttc cccccggtga aaatataaaa
     9001 ttacttgcca gaagcgattc tccgatagtg ggaagcctcg gcaaataact gatgatatca
     9061 acattaaacg aaccaataac gcatacttga cttttcatag ttgagtggcc tttttttatt
     9121 ccctgggctg tatcataatt ttcaagcgca taagcgattt catttttcgc aatagtatca
     9181 agcgagtcta ggacgataaa agcaccgcca tgacatcgag tcagatagcc aagcctggca
     9241 agttcattaa gatctgaacg aatagtttct ttcgttacat tgaggctatt ggacagatcg
     9301 gatactttta cagaaccgaa tttactgagt cgtttcaata tttcatgatg gcgttcaaat
     9361 ttcatgcttc acctcactgc agttacgtta atatctatcg atgtatattg atgtgataat
     9421 caccataaag tggcggggaa aagcaaaaaa aagcaaaaat agcctgagaa aaggtaagcg
     9481 ttaatggaga atcatattga tgcttattta ttggtgagaa ctacgttcca tggcaggagt
     9541 tcatcaatac ggttggaagg ttattccggc agtacgctca ggatatggcg cagatacgct
     9601 tccggatcaa tgtcgttcag aaggcaagtg ccgataagcc catacaacag tgcactgcgc
     9661 tcgccgccga agaacataaa gtttttattt ccgagacaga tggcacaaag ggctttttcc
     9721 gcggcattat gtctgcctcc gcctgaccgt caacactgta ataacagagt gcgccccact
     9781 gattcagtac acagctgaac cattctccca gtctggattt ttggatagcg tggcattttt
     9841 ctccaccatg catgcatgca gtgagtccag taacagtttg ctcttcttct gcctgaccac
     9901 aagatgtgct ggactggtaa acgatgggga tacctgctta cgcgagagca ataagtggct
     9961 ggccacaatt ctcggtacaa aactctgctg aatagcagcg attttcccag cgtggtgcgt
    10021 cgtttgcatt ttactggttc taagttaaca attatctgtt cactaatgac tactctaaaa
    10081 acttgtatca ggactatcac ttatttaagt gatattggtt gtctggagat tcagggggcc
    10141 agtctataaa ctttgaggta gcctg
//
LOCUS       DBBGLQ010000137         9806 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.137, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000137 DBBGLQ010000000
VERSION     DBBGLQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.137"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(222..980)
                     /gene="yhdZ"
                     /locus_tag="ACIYSB_004669"
     CDS             complement(222..980)
                     /gene="yhdZ"
                     /locus_tag="ACIYSB_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGC5431552.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLAVHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(988..2091)
                     /gene="yhdY"
                     /locus_tag="ACIYSB_004670"
     CDS             complement(988..2091)
                     /gene="yhdY"
                     /locus_tag="ACIYSB_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGC5431553.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQYLEKRFNTGRTPH"
     gene            complement(2101..3282)
                     /gene="yhdX"
                     /locus_tag="ACIYSB_004671"
     CDS             complement(2101..3282)
                     /gene="yhdX"
                     /locus_tag="ACIYSB_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGC5431554.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3350..4375)
                     /locus_tag="ACIYSB_004672"
     CDS             complement(3350..4375)
                     /locus_tag="ACIYSB_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGC5431555.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4806..5027)
                     /locus_tag="ACIYSB_004673"
     CDS             complement(4806..5027)
                     /locus_tag="ACIYSB_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431556.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5280..8384)
                     /gene="acrF"
                     /locus_tag="ACIYSB_004674"
     CDS             complement(5280..8384)
                     /gene="acrF"
                     /locus_tag="ACIYSB_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HGC5431557.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8396..9553)
                     /gene="acrE"
                     /locus_tag="ACIYSB_004675"
     CDS             complement(8396..9553)
                     /gene="acrE"
                     /locus_tag="ACIYSB_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGC5431558.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2616 a         2531 c         2349 g         2310 t
ORIGIN      
        1 aacaaaaggc tcagtcggaa gactgggcct tttgttttat ctgttgtttg tcggtgaaca
       61 ctctcccgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
      121 gagggtggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag cgaaaggcca
      181 tccgaaagga tggccttttt gcttttcgaa ctaacattca attaatggat tacctgcgat
      241 aaaaatgccc tcgtacgctc tgatttagga tgcgcaaaaa attcatcagg tgcagcttgc
      301 tccactattt ccccacgatc cataaaaatt acccggtcag cgacggttcg tgcaaacccc
      361 atctcatgtg ttacacacaa cattgtcata cccgactgcg ccagcccaat catcgtatcc
      421 agcacctctt tcaccatctc aggatcgagc gccgacgttg gctcatcaaa caacataatt
      481 ttcggcttca tacacagcga acgcgcaatg gcaacgcgtt gctgctgacc acctgaaatc
      541 tgtccgggaa acttatgcgc atgttcggca attctcaccc gctctaggta atgcaccgcc
      601 agatcttcag cctctttctt aggcatcttg cgtacccaaa tcggtgccag ggtacagttc
      661 tgtaaaacgg tcagatgagg gaagagattg aaatgctgaa agaccattcc cacttcctgc
      721 ctgacgcgct caatattgcg gatatcttca ttaagttcga tgccatctac cacgatccgt
      781 ccctgttgat gttcttccag atgattaata caacgaatgg ttgtcgattt accggaacct
      841 gaagggccac acagaacgat ccgttctccc ggttgcacgg ttaaatttat atttttcaaa
      901 acatggaatt gtccatacca tttattgacg ttttccagcg taatcatcgc gttagcaggt
      961 tgcagtaaaa tttggctcat agtgtcctca atgcggtgta cgcccggtgt taaaacgttt
     1021 ttccagatac tggctatagc gcgacatgct gaaacagaag atccagtaga tcagcgcggc
     1081 gaaaacatac ccttccgtcg acatacccaa ccaggcggga tcaacggttg cctgctgaac
     1141 gctactgaaa agatcgaaca acccgataat gatcaccagg ctggtatctt tgaagagtgc
     1201 gatgatggta tttaccagcc caggaattac cagcttcaac gcctgtggca gaataaccag
     1261 cccctgagtt ttccagtaac ccaacgccag cgactctgcc gcttcatatt gccctttagg
     1321 cagcgcctgt aatccacctc gcacgacttc cgcaacatat gctgactgaa acaggatcac
     1381 gccaaccagc gcgcggatca atttgtcgat actggtgcct tctgccataa acaacggcag
     1441 catgaccgaa gacataaaca gaacggtaat cagcggcacg ccgcgccaga attcgataaa
     1501 aatgaccgat aagatacgca caatcggcat atgggagcgg cgacctaacg ccagtaagat
     1561 cccccacggt agcgcccccg caatcccaac tgatgcaata attaaagtta gcgtcagccc
     1621 gccccattgc cgggtttcaa cccgctcaag agcaaaaaaa ccgccataca tcagccacca
     1681 gacaatcagt gggtaaatca ccgcccaggc cgcaatatag cgaccgcgat gcgggagtat
     1741 tttccagaac attggtgcga tcgacacaag cccaatcagt aatgcgaggt taattcgcca
     1801 gcgttggtcg tgtgggtaaa gcccatacat aaactgacca aatcgttcgt ggatgaagac
     1861 ccaacaggca ccggcttttg tgcagtcggc acgcgttgag ccaacccagt tagcctgcaa
     1921 aaatgcccag ttcagcaacg gtgggatcag ttcccacatc aaccatatgc agccaatagt
     1981 cagcaggcta ttgctccagc tggagaacag attttttcgc acccacacca tcgcgcggct
     2041 tgagttatgg ctcgccgggc gcgggggatg agacagcaat acttttgtca tcatgactcc
     2101 ttagcgttca acgatcgcga tgcggcggtt atagatattc atcagcagcg agatagtcag
     2161 gctgataatc agatagaccg acatggtcat ggctatcgtc tcgatggctt gccccgtctg
     2221 attcagcacg gtgccggcaa acagcgaaac catatcggga tagccaatag cggcggcaag
     2281 agaggagttt ttgacgatgt tgagatactg gctggttaac ggtggaataa tcacccgcaa
     2341 tgcctgggga ataatgacct ggcgtagcgt aaccgggttg ggtaatccca gtgaccgagc
     2401 cgcttcatgt tgaccataag gcactgcctg gatcccagcg cggataatct cggcgataaa
     2461 tgcagatgta taaaccgaaa gtgccagcgt taaggctgcc agttcaggaa ttaaaaccat
     2521 cccgccgcgg aagttaaagc ctcgtagggc tgggacatcc cagtgtagtg ctgcgccaaa
     2581 aagccattgc gccagtaaag gcaaaccaat gatcaacact gcggcgatcg gccaggtgcg
     2641 gcgcagttgt ccggtcttta tctggtatgt tttattaaag cggaatagcc caacagaaag
     2701 gactatagcc ataacaacag ccaggataaa cgcaataaat ccgtctccca gctgcggtga
     2761 cggaatataa agcccacgat tgctcaaaaa ggccagatca aacgcgctca cagcctggcg
     2821 tggtccgggc aaattgcgca acacggcaaa gtaccagaag aagatttgca gcagcggggg
     2881 aatattacgg aagatctcaa tataaattgt ggaaagcttt cgtagcagcc agttatccga
     2941 aagtctcgcc agaccgataa agaagcccaa aacagaagcg aacacaatac acaatgcaga
     3001 aaccagtagc gtattgagta agccgacaat aaaaacgcgt ccgtaggtgt cgccctgctg
     3061 gtaatcgatc aaatgctgga caataccgaa gccagcgccg cgatccagaa aggcaaaacc
     3121 tgaagtaatg ccacgattat tgagattcgt tacagtgttg tgaaataacc aaccaacaat
     3181 gccgacaaca gcaacaacgg caaggatctg gaataaccag gcgcgaaccg tagggttggc
     3241 aaaagagagt gagcctttaa cggttgagcg gcgatgagac atagaaacct cggaaagcat
     3301 aacgctgaag agagcgccgc cgtagcgacg ctcgttacct cacaccgact taacgcacgg
     3361 gcggtgcgta ctgaataccg ccgttattcc agagattatt ttgcccacgt ttaattttca
     3421 gcgggctttc tgaacctacg ttacgctcaa aaatttccga gtagttaccc acctgtttga
     3481 tgatgttata ggcccattta ttatccagct tcagatcctt gccgtaatcg ccttctttac
     3541 ccagcagatg tgccatatca ggcgttgctg gattagccgc tttttcatcg acgttctggg
     3601 aattgatgcc catctcttca gcattcagca tggcgaaaag cgtccagcgt acaatcgaga
     3661 accattcatc atcgccacga cgaactaccg gaccaagcgg ttcttttgag ataacttccg
     3721 gtaagacaat ccattcagct gggttgctta atttgatgcg cagggcatac agttgtgatt
     3781 gatccgaggc cagcgtatcg cagcgaccag attccagtgc cttcgctgat tcgtcagagc
     3841 gatcgaaagt caccggtgtg tacttcatat tgtttgcctt gaagtagtcg gcgacgttga
     3901 gttcggtatc agtacccgcc tgaatacaga cggtagcgcc atccagttct ttcgcgcttt
     3961 ttagccccgc tttatcgtgc gtcaggaagc caatgccgtc gtaataagtg acgccggtaa
     4021 atgccattcc catcccggca tcgcgagatg aagtccaggt cgtattacgg gagagcaaat
     4081 ccacctcccc tgactgtaaa gcggtgaagc gttcttttgc agtgagcggg gtatatttca
     4141 ctttcgtgtc gtcaccaaat acagcagcgg caacaccacg acaaatatca acatcaatac
     4201 ctgaaaactt accgtcagca tcggcataag agaacccagg taatccatca ctgatcccgc
     4261 attgcacaaa accttttttc tgaacggcat caagcgtcgc gccagcatgc gcctgatttg
     4321 caacggcaag cagcacgctg gcggcagcca gtgtggctat catcatcttt ttcataatgc
     4381 ttcctgtgag gcgaaaatta tcgttatagg acgtttggga aacgtctttt acctgtctgt
     4441 ggccatcctc ttataagcaa aagtagtgcc agcttttgcg cacgatttcg cgtcgcaata
     4501 agctgagtgt agacaggaaa taataaaaac agcgccccgc aatggtgcga aaatgaaaca
     4561 tgcgccacat tttggcgcaa aatatttacg cgtgttttac taagcagaat aatgattgtt
     4621 agcgtgagta tatttctggt gggaagtgtg aaaattaaat tgatgcgtta tttcgaaagc
     4681 agcgaaataa cgcgatgtaa catgctaaat cagcagggaa ttacccagca gaatatagca
     4741 atattttatc ttatggagag atactgtatc gttaaaaaga gcgcggaact ccgcgccctg
     4801 ttcagttatt tcgatagagc gatgatcccc atcgtcaggc cagcaacagc agctgccccg
     4861 ccggcaatag ccccagccgt ttcccagtta tcctgagtcg tgcctttcat acaggtattg
     4921 gtgtgaacca gacgaccctg atcgtcataa accggaacgc agggcgaatc gtgagcacag
     4981 cccgccagca atgcggtgag caatgccaca ggaattaatc ttttcatggt gttacctcag
     5041 taataaacgc taacgcttaa taagttaact ctcttattaa cgagctgaat gccattttat
     5101 caccggaggt tataaatctt gcggaatgaa taatgacaaa ttatgtgaat tgtaaaaata
     5161 gtgaagggag cgggaactat agaaagcttt taaagagtga gcagaagaat atcttattaa
     5221 tttttatggg tttcactgga aataataaag gcacccgaaa gcgcctttat gtttctgatt
     5281 tatcctttaa agcaacggcg gatcaccaca aagaacaccg gtacgaagaa gattgccagc
     5341 aacgttgcag agaccattcc tcccattacc ccgataccca cagcgttctg cgcgccactg
     5401 ccggcaccgt tactgatagc tagcggtaat acgccgagaa taaaggcgag agaggtcatc
     5461 aggataggac gcagacgcat acgtactgcc atcagtgtcg cttcaacaac acctttaccc
     5521 tctttctcca tgagatcttt agcgaactca acgatcaaaa tagcgttttt ggccgacaag
     5581 ccaattgtcg ttagcaagcc caccataaag tagacgtcat ttttttgatt aaagagtgtc
     5641 gccgccagca gcacgccgac aatccctaac ggcactacca acataaccga gacaggaatt
     5701 gaccagcttt catagagtgc agcaaggcac aggaaaacaa ccacaaagga aattgctacc
     5761 agagcgggag cctggtttcc cgataagcgt tcctgatacg acatacccgt ccagtcataa
     5821 ccaatgcccg caggtaattt tgacgcaagg ttttccatca acgccatggc atcgccggaa
     5881 ctggttcctg gcgcggcttc cccctgaatc tccattgacg gcagaccgtt gtagcgttcc
     5941 agtcgcggag agccatacac ccaatgtgaa gtggtaaagg ccgagaatgg caccatttcg
     6001 ccgttggcgc tgcggacata aagtttatcg acatcttctg gcagcatacg gaatttggca
     6061 tccgcctgaa catacaactt tttcacgcga ccacggtcga tgaagtcgtt aacgtaagtc
     6121 ccacccagcg ccgttgaaat ggtctgattg atgtcagaaa gtgaaacacc taatgcctgc
     6181 gccttttcct ggtcaacttc cagtttaaac tgcgcggtgt cttccaggcc attagggcgc
     6241 acgctgacta agctggcagg atgttgcgcc gccataccaa gcaactggtt acgggcctgg
     6301 gttagggcat cgtgacccag cccagcctga tcaattaact caaagtcgaa acccgttgcc
     6361 gtgcccagtt caacaatggc tggcatattg aatggaatga caaaaccgtc gcggatcttg
     6421 cccaattcca ttttggcacg atggattacc gcttccgcac tgttttcgtc accattacgc
     6481 tcttcccacg gtttcagact gacgaaggcc ataccggcgt tttgtgcctg gccgctgaag
     6541 ctaaagccgt taaccgtaaa gacactttca acgttcgctt tctcgttctt cagatagtaa
     6601 tccgtaactt gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc gggtaactga
     6661 atcatggtca gaaagacacc ctgatcctct tcaggtaaga aggaagacgg aagacgtaaa
     6721 aacaacacca ccattcctgc aacaatcagc gcatagatca gtaaatatcg tcctgtggat
     6781 ccgaggattt tgccgacgct gttggtgtag tggttaacgc tatgatcgaa ggtggtatta
     6841 aaccaaccga agaaaccgcc cttattttcg tgatgctcag cagagacggg tttaagcagc
     6901 gttgcacata acgcaggggt aagaatcaat gccaccagaa cagaaagcgc cattgccgaa
     6961 acgatggtga tagagaactg gcgataaatt gccccagtag aaccgccgaa gaatgccatc
     7021 ggaataaata ccgctgacag caccatcgcg atacccacca gtgcgccctg aatttgcgac
     7081 atcgattttt ccgtcgcttc ttttggcggg agcttatcct ccatcatcac gcgctcgacg
     7141 ttctccacca ccactatcgc atcatcgacg agcagcccga tggcaagcac catcccgaac
     7201 atcgttagtg tgttgatgga gtaaccaaaa gcggcgagga tggcaaacgt ccctaacaac
     7261 accacgggta ccgcaatggt ggggatcagc gttgctcgca tattctgcaa gaacagatac
     7321 atcaccagga acaccagcat aatggcttcg aacagcgttt ttaccacttc gtgaatagaa
     7381 agctggacga atggcgtggt gtcataaggg tagagaacct tcattccctg cgggaagaat
     7441 ggctgtaatt ccgccagttt tgccttaatg gctttcgcgg tatcgagagc attcgcgccg
     7501 gttgccagct taatccccag gcccgccgcc ggttttccgt tgatacgagc gataacgtta
     7561 tagttttcac cgccaagttc aacccgtgcg acatctttca ggcgtaccac cgagccgtca
     7621 ctgtttacgc gcagggtcac tttgccgaat tcttccggat ttttaaaccg cgtctgagca
     7681 ataatcgagg cgttcaattg ttgccctggt aacgctggcg ttccgcccaa ctgtccggca
     7741 gcgatctgat cgttctgtac cttcaactgg ttaatcacat caaccggtgt cagtttatat
     7801 ttgtttagca gatcggcatc cagccagata cgcatcgcat actgtgcgcc gaaaagctgt
     7861 acgtcaccga cgccattcag acggctaagc gtatctttaa cgttagaggc cacatagtcc
     7921 gagatatcgt cctgtgtggt gcctgggtta tcagagacaa agcccgccac catcaaatag
     7981 ctgctactgg acttttcaac actgatcccc tgctgctgaa cctcctgcgg cagcaacggc
     8041 gtggcgagct ggagtttgtt ctgcacctgc acttgcgcga tatcaggatc ggtcccggac
     8101 tggaaggtaa gggtaattgt cacgctaccg gcggaatcgc tggtggagga catatacatc
     8161 aggttatcga taccgttcat attctgttcg ataacctgcg tcaccgtatc ctgcacggtc
     8221 tgcgcatcag cgcccggata gtttgctgaa acagaaaccg caggcggtgc aattgttgga
     8281 tactgagcga cgggcaattg taggatcgcc agtgcgcccg ccatcatcag aataatggcc
     8341 agcacccatg caaatatcgg tcgtcgaata aaaaagtttg ccatgtcaga ttaccttact
     8401 tcgatgcagt atctgcgggg gtatcggtag tggctttaac ctgctctccc ggacgcgctt
     8461 tttgcaggcc gctgacaatg acttgatcgc cagatttcag tccttcacta atcaaccatt
     8521 tatcgccaat cgcctgactg gcaacgacag ggcgcgcttc aacctgactt ttatcgttaa
     8581 caatcagcac ggttgcatca ccacgcggtg tgcggctaac gccttgttgc gggataagaa
     8641 tggcgtcagg ttggacgcct tcatcaatcc gtgcacgcac aaacataccc ggcaaaagcg
     8701 tatgttgcgg gttagggaag acagcacgta gggttatgga gccggtgctt tcatcaacgg
     8761 tcacatcgga gaattgcagc gtacctttca ggggataggt ttgaccgttt tccatgacca
     8821 actctacgtt gctggtggcg ttttccttat gcaaatttcc ttgctctacg gattgcttca
     8881 gcctcataaa atcgttgctg gattgggtca catcaacgta gataggatcg agctgctgga
     8941 cagtcgccag ttcagtcgtt tgcccattag tgacaagagc gccttcggtc acagtcgatt
     9001 tgccgatacg tccgctaatt ggcgcagtga ctttggtata agcaagattg atgcgagcgc
     9061 tttcgactgt ggcttttgcg gcaatcacgg cggcatcggc ctgacgagca tcagcaatgg
     9121 cctggtcgta ctcctgctgg ctgatgtatt tcgtacccac gagcggaacg taacgtttta
     9181 ccgtcaaatg cgcgatggcg gcggcggctt cacttttcgc cagttcgcct ttcgcgctgt
     9241 cataatttgc ctgataggtc gcgggatcga tctggtacag ggactggcct gcttgcacat
     9301 cgctgccttc agtgaaattg cgattcagta cgatcccgct aacctgtggg cgaacttcgg
     9361 ctatacgata agcattggtg cggcctggta attcagtctt aacttctaac ggggccgttt
     9421 ttacaatatg aacggtaacc tgcggttcac cgacgtgagc tttctcttct cccttatcgt
     9481 tacaaccggc gattaaagcc gcggagatca gaataaagga gggcaggagg aaaaacctgg
     9541 catgtttcgt cattactatt cctcaaaaaa ccaaaagcgc gttatttacc caaaaaggca
     9601 acgcgttaac tcgcagaaag aaaaatacag ttcgctatcc tacaaattat cattcgtcga
     9661 tgtaaggaat agttatgaat acaggcatct caaggcacat aaacacaaaa aaagattaat
     9721 attctactgt tttattttga cgcgggttga aagaggcaga attaaaacct cgtaaattga
     9781 aatatatatt gatgtagtga atgtat
//
LOCUS       DBBGLQ010000138         9766 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.138, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000138 DBBGLQ010000000
VERSION     DBBGLQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.138"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            447..1142
                     /gene="minC"
                     /locus_tag="ACIYSB_004676"
     CDS             447..1142
                     /gene="minC"
                     /locus_tag="ACIYSB_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGC5431559.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1166..1978
                     /gene="minD"
                     /locus_tag="ACIYSB_004677"
     CDS             1166..1978
                     /gene="minD"
                     /locus_tag="ACIYSB_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGC5431560.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1982..2248
                     /gene="minE"
                     /locus_tag="ACIYSB_004678"
     CDS             1982..2248
                     /gene="minE"
                     /locus_tag="ACIYSB_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGC5431561.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2620..2963)
                     /locus_tag="ACIYSB_004679"
                     /pseudo
     CDS             complement(2620..2963)
                     /locus_tag="ACIYSB_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2998..3117)
                     /locus_tag="ACIYSB_004680"
     CDS             complement(2998..3117)
                     /locus_tag="ACIYSB_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGC5431562.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3078..3263)
                     /gene="ymgJ"
                     /locus_tag="ACIYSB_004681"
     CDS             complement(3078..3263)
                     /gene="ymgJ"
                     /locus_tag="ACIYSB_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HGC5431563.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYQKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3364..3537
                     /gene="ymgI"
                     /locus_tag="ACIYSB_004682"
     CDS             3364..3537
                     /gene="ymgI"
                     /locus_tag="ACIYSB_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HGC5431564.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3539..3883
                     /gene="ymgG"
                     /locus_tag="ACIYSB_004683"
     CDS             3539..3883
                     /gene="ymgG"
                     /locus_tag="ACIYSB_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HGC5431565.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3887..4222
                     /gene="ymgD"
                     /locus_tag="ACIYSB_004684"
     CDS             3887..4222
                     /gene="ymgD"
                     /locus_tag="ACIYSB_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HGC5431566.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4279..6926)
                     /gene="ycgH"
                     /locus_tag="ACIYSB_004685"
                     /pseudo
     CDS             complement(4279..6926)
                     /gene="ycgH"
                     /locus_tag="ACIYSB_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YcgH"
     gene            complement(7326..7544)
                     /gene="ymgF"
                     /locus_tag="ACIYSB_004686"
     CDS             complement(7326..7544)
                     /gene="ymgF"
                     /locus_tag="ACIYSB_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HGC5431567.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(7676..9199)
                     /gene="pdeG"
                     /locus_tag="ACIYSB_004687"
     CDS             complement(7676..9199)
                     /gene="pdeG"
                     /locus_tag="ACIYSB_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGC5431568.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEVVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECFNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(9531..>9766)
                     /gene="ymgC"
                     /locus_tag="ACIYSB_004688"
     CDS             complement(9531..>9766)
                     /gene="ymgC"
                     /locus_tag="ACIYSB_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HGC5431569.1"
                     /translation="PERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSV
                     ILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         2781 a         2209 c         2094 g         2682 t
ORIGIN      
        1 ctggacgagt tacgatgacc tgacagaagt atcagacgtc ttctgcggtt tctatgatag
       61 agcaattctg tggcggatat taagataacg ggtatctgtt ctttaatgat gctgcaaaaa
      121 atggaaagtt aagaaagcct ggcgtggtga aaaaatacac ttgtctcaat actggatatt
      181 taaacaggtc tggagatgct gtcacgatta gggtgcggaa aaaggaagcg taaataagaa
      241 gaatcaaaag tacgacggca atgggttgat tgacaagggt attttttaag ctatgaatca
      301 gcgccattta tcacagaata gacttttact ctgaataaat gggagggtga cttgcctcaa
      361 tataatccag actataacat gccttatagt cttcggaaca tcatcgcgcg ctggcgatga
      421 ttaatagcta attgagtaag gccaggatgt caaacacgcc aatcgagctt aaaggcagta
      481 gcttcacttt atctgtggtt catctgcatg aggcagaacc taaggttatc catcaggcgc
      541 tggaagacaa aatcgctcag gcccccgcat ttttaaaaca tgcccccgtt gtactcaacg
      601 tcagtgcact ggaagacccg gtaaactggt cagcgatgca taaggcggtt tcggcaaccg
      661 gtttgcgggt tattggcgta agtggctgca aagatgcgca acttaaagcc gaaattgaaa
      721 aaatggggct gcctatcctg acggaaggaa aggaaaaagc gccacgtcca gctcccgcac
      781 cgcaggctcc agcgcaaaat acaacgccgg tcacaaaaac gcgtttaata gataccccgg
      841 tgcgttccgg tcagcgtatt tatgctccac aatgtgatct gattgttaca agccacgtta
      901 gcgctggggc cgaattgatt gccgatggga acattcatgt ctatggcatg atgcgcggtc
      961 gtgcgctggc aggggcaagt ggtgaccggg aaacgcaaat attttgtacg aacctgatgg
     1021 cggaactggt gtccatcgca ggtgaatact ggctgagtga tcaaatccca gcagaatttt
     1081 atggcaaagc ggcgcgactg cagttagtcg aaaacgcttt gaccgttcaa ccgttaaatt
     1141 gatccctttt taacaaggaa tttctatggc acgcattatt gttgttactt cgggcaaagg
     1201 gggtgttggt aagacaacct ccagcgcggc catcgccact ggtttggccc agaagggaaa
     1261 gaaaactgtc gtgatagatt ttgatatcgg cctgcgtaat ctcgacctga ttatgggttg
     1321 tgaacgccgg gtcgtttacg atttcgtcaa cgtcattcag ggcgatgcaa cgctaaatca
     1381 ggcgttaatt aaagataagc gtactgaaaa tctctatatt cttccggcat cgcaaacacg
     1441 cgataaagat gccctcaccc gtgaaggggt cgccaaagtt cttgatgatc tgaaagcgat
     1501 ggattttgaa tttatcgttt gtgactcccc ggcagggatt gaaaccggtg cgttaatggc
     1561 actctatttt gcagacgaag ccattattac caccaacccg gaagtctcct cagtacgcga
     1621 ctctgaccgt attctaggca ttctggcgtc gaaatcacgc cgcgcagaaa atggcgaaga
     1681 gcctattaaa gagcacctgc tgttaacgcg ctataaccca ggccgcgtaa gcagaggtga
     1741 catgctgagc atggaagatg tgctggagat cctgcgcatc aaactcgtcg gcgtgatccc
     1801 agaggatcaa tcagtattgc gcgcctctaa ccagggtgaa ccggtcattc tcgacattaa
     1861 cgccgatgcg ggtaaagcct acgcagatac cgtagaacgt ctgttgggag aagaacgtcc
     1921 tttccgcttc attgaagaag agaagaaagg cttcctcaaa cgcttgttcg gaggataagt
     1981 tatggcatta ctcgatttct ttctctcgcg gaagaaaaac acagccaaca ttgcaaaaga
     2041 acggctgcag attattgttg ctgaacgccg tcgcagcgat gcagaaccgc attatctgcc
     2101 gcagttgcgt aaagatattc ttgaggtcat ttgtaaatac gtacaaattg atcctgagat
     2161 ggtaaccgta cagcttgagc aaaaagatgg cgatatttct attcttgagc tgaacgtgac
     2221 cttaccggaa gcagaagagc tgaaataagc ccgctgtaaa agcgcattta tcttcaaggc
     2281 agagttatct ctgccttgag tttttcatcc ctctcatcca cgttgtggta aagcggcgag
     2341 tattcttgct gatactcctc attgctattt ccacctcccc ctttacacct taaggctgtt
     2401 cccacgcaca aaacaaaatg tttatgcctg gttgagtaaa taaccttatt gttagtatgg
     2461 atatactggg agatgataac cccgcaattt cattattcat tgttctccca taggatgagg
     2521 tttcccgccg taactggcgg gctttttttg ccccagaatt ttccctttca acatcctgta
     2581 aacgaaaact gcgccgaagc gcagtttaat cgttgatggt cagaacgacc agttcacacc
     2641 agccaccgcg ttccacgggg attccacacc ggcaccatgg ctatacccca ccccgagatg
     2701 tccgctaaac gtactgctga atgaggcttt aatagctgcc tggtatattc cacgtctgcc
     2761 cgacaaatca ttgacgaaat taccgtcatt attcaccttc acccggttat catcgacaaa
     2821 ttctttgcgc acagccgcct tcagccaggg ttcatttcca taccgttccc caggtgcatg
     2881 ttgtaactca gcgttgcacc cagttcacga tatatactgc gggtatcgac tgatttcgat
     2941 tccatgccat tggataaatg atagtagcgt ctaagtgacc taagctctgc tgcaacatca
     3001 gcccgcaggc accagaccag gggattcatc ctgaagagac agcgcaggtg tattgtgttc
     3061 accgctcacc aaagacatca tgatgaaatg atgatatccc gcataagaaa gaggcatttt
     3121 ttacacgcag tgcgctgaag tgtagttgga taaaaaatca ctcttttctg ataatactgt
     3181 cgtacgctta tctttcgaca atgaggccgt cggcaaaata aaatgactaa tgtaatcgtt
     3241 gctgatgaat atcttctgct cacataaaaa tcacacaata atattgataa atcagattat
     3301 cttaccttta caacattgcg ttaacagtca atgacacttc ttgaataaaa atattgtcaa
     3361 tcaatgagtt attcctcatt taagattatt ctcattcacg taaaaaacat aattcctatc
     3421 atcacagcca cgctcatttt gaattatctg aataatagcg agcgttcttt agttaagcag
     3481 atcttaattg aagacgaaat tattgtgtgt gcaacttact taataccgga catctaagtt
     3541 gaagaaaaaa atacttgcct tcgggcttat ctcagcattg ttctgctcca cgccagcgat
     3601 ggcggatatg aaccgcacca cgaaaggtgc attgctaggc gcaggcgttg gtttacttac
     3661 tggcaatggt gttaatggcg tactgaaagg tgcagctgtt ggcgctggtg ttggtgcagt
     3721 aacagaaaaa ggccgcgacg gtaaaaatgc gcgtaaaggt gcaaaagtgg gtgccgccgt
     3781 tggtgctgtc actggcgttc tgaccggcaa tggcctcgaa ggggcgatta aaggtgccgt
     3841 gattggcggt acgggtggtg caattctggg taaaatgaaa taaggaatga tgatgaaaaa
     3901 atttgcttta ctggcgggcc tgtttgtttt cgcaccaatg acctgggcgc aggactacaa
     3961 tattaaaaac ggtttgccgt cagaaaccta tattacttgc gcagaagcca acgaaatggc
     4021 aaaaactgac agcgcacagg ttgcagaaat tgttgcggta atgggtaatg ccagcgttgc
     4081 cagccgtgat ttaaaaattg agcaatctcc ggaactgagc gccaaagtgg tagaaaaact
     4141 gaatcaggtt tgcgctaaag acccgcaaat gctgttgatc acggcaattg atgacaccat
     4201 gcgtgctatc ggcaaaaaat aatatttcat atgtaccgcg tcgatgaata cgacgcggtt
     4261 ttttagctaa cggcgggatc agaacttata tttcaggccc accatcactg cggtgtcatt
     4321 gtagccatta tcacccagct gcacgcccac attccccaga cgctggccgc tggattcagg
     4381 ttgccattta cgccaagttt aatttccccc aaattactcg ccccatcttg ttcgactttc
     4441 acaccgttca ttgagacagc aaaatcttta ctgttatgga tccagttcat ttcaacaaaa
     4501 ggttcaaagt cgtcaccgtg cggaccgctg tttttctcct gagtacgaat aaaggtgcgg
     4561 aagccgagtc gggtctggat gttgttattt gcgtcattct caatacgtga gccattggct
     4621 tccgtgtgcg cattatgatc gacgcccatc cagatagcct gtgcctgtgg ctggaagatc
     4681 cacgtatatt tcgccgcttc cagaccaaag gatttattca gggcaaaacc gtaacccgct
     4741 tccagcgaga cggttgcacc tttcgcgcta taggattcat aggctaattc atcccctttc
     4801 accgagtttt taaaccagct atattgcgcc caggagtcaa tgtatgcgcc ttttttactg
     4861 atgtcatcgg caaaccaggt ggcatacagc cctgcgctat agcctctgac actccctttc
     4921 gaacgataat ccgacacgct ggaatgagtt aagttgtagt cgcgggcata accagccatc
     4981 actcccaaac gccaactgtc actatcggta aaaccaccgg ttaacagatc gccccctaac
     5041 tgcgagacgt agcgatgcga ggtcgttctc aactgtccgt tgctgtcacg ccaggcatta
     5101 tgcccgccaa tttgacgtag ccaaagtcgg cttgaacgct cctgttcagt tacaggatcg
     5161 atgtagcgcg tttcacccgc acggtcgttc agacgcataa caaaaagagt gttagcagct
     5221 gccaggttag cgacgtaact tccagcttcg gggcgcagga cgggggtagg cgtttctgtt
     5281 gattctgaat ttccctctga gctgttatcc tcctcattca ctgactgata acttgtcaaa
     5341 taccagtttt tgccttgttg ttttagttga tattcgaaag ctccagctgc aatccttgca
     5401 tcaccttgtc tgaaggcatc tgctggagat tcatcgccat tcacagttat gagttcgatc
     5461 ccgtttagcg tggccgcgcc gcttcctcca gcatttttca catagacggt agttgaaccc
     5521 gaagtattac cctcaacaat aagtctgtcc gtagccgatt tatcatcacc taatacggtg
     5581 cggatattaa gctgaccatt attaccgaca taattacctt ttacggttaa tgtttttcct
     5641 tgggtaaatg tttgaccatc atcatacgaa agattgataa tactgtctga attagtcaga
     5701 tgggtaacaa cggagtcatc tttcatattc cagattgtat tttctgaaag cttcagcgtg
     5761 gttgtgccgc tgacgagttt agtcgctcca tttagaatag agtcggacga aattatgttt
     5821 aagtcacctc cagtattatt aaccagaaca ttattaccag gtgtgataat acttcgatta
     5881 aggatactta catccgttgt agttttgtta acatcaatac tatccaaagc acttgaaagt
     5941 tcaacactta atagtgctat tttggcttga gaatcgatat ttgatgcatc aattgatatt
     6001 cctttatctt attttgatac cgcggtgaca ttggaaaaaa ataactctcc accattgtat
     6061 atttttgcaa tgggggcttt agtgcctgtt gttgtaatag aactattttt tatatcaatg
     6121 ttaccttgtc gaatagcaac tgcataactt ccatcagcca cagtttcaag tgccacataa
     6181 tctaaattaa tatatgcttt tttcccatta gcataagcac catagctatt aatgccatta
     6241 gttgttatgg aaccaccatt catttttaaa tcagcacggc taataacttc gtaactgtta
     6301 gaatcactaa cttcatcatc tacagcatcg acacatgttc gagactctcc aggacttgaa
     6361 catgcaagaa cacctactgc attttcccca gaggtaacaa tgttcgtttt atctgtggtt
     6421 tcaagacggg tgttatttac catcttttca gaatcattaa cctggcttaa aagtcccgct
     6481 gaatatgccc cacccgactc gactgaatta tcaccattca taaagatggt gccaccaaat
     6541 tcactgacta ctgcgctggc tgttatccct ccattttctt tgtcagatac gcttccagag
     6601 gtcttaatat cgctatcgct tatatataat actttgtttt gactatcaac agaaatacca
     6661 taagatccat caccactagt catcacactg gcattattta tatatccagt tacaccgtca
     6721 tccactttca cagcatggct ttcgtcttca gtggttagat actctccatt acttatcgga
     6781 ttattatctt tgatttcaat cggcgattct tcatcagtca tataggaaat tatactataa
     6841 tcatccaaca attgtgttgc atatgcatta cccaccgcca gagcaataag tcccaaacta
     6901 aacccgggga gttttttcag tttcatagat taaccatcca ttcattggat taacaaacat
     6961 tcctggtcaa ataacctatg agctcagatt accaaaacgg agggtaaaga caaaaacaaa
     7021 agcacaaaaa atttaaatag ataaacagcg acaatatata aatgataaat aagtcggcat
     7081 tatcattttt caccttattt tcatgacatt gatcactttg aggtgacgat tttgtttaac
     7141 aaataactct tcgacatcaa aattgttaat agttgcgatg aggattattt cggtgatata
     7201 taaaaacaac cgtgataaca ccagggttga caactatttg cgcaataatg cctttccttc
     7261 aatgatccag gcgttcactg ccagcaaccc attcagccgc atactgacag tgagaaactg
     7321 aaagattatt gctgatccct gtggttgacg agtctttctc ttaatcgatt aaccagattg
     7381 atcgccagcc aaacaagggc attaagcaaa tttatcgtca caagcacttc tcccagcccg
     7441 gtactgggtt ttgcaataag gatgaccagg agggtgatca gcataaatgc aatggaaaat
     7501 atgatataga gagtgaaata atccagatta ttactattgt tcatgctact aatggcaatc
     7561 ctgtcaaaac tttcaaacaa ttatagcaaa caaaataatg aacaatagta acaagaggca
     7621 acgagctaat tagctgtcta taattacacc gcactatggc cttactgatc aattttcact
     7681 caaccacaac cttcaccttc ggtttagaaa ggataatttt gaccaggtcg atgtatgtaa
     7741 caggtttata gaaataataa ccctgctgaa atgtgatatt attttggttc agatagtcaa
     7801 gttgttcttt cgtttcgaca ccttcagcga cgatactgat cgaaagttta cgcgccagat
     7861 ccaatacaca atccagaatt cgggttgatt ctgggtctgc gtttacgcgg ccaacgaaac
     7921 tgtgatcaat tttgatataa tcaatatgca ggtcatgaag ataagaaagc cctgagtagc
     7981 cagtaccgaa atcatccagc gcgatgacaa aaccattttc atgcagtatg ttcaaccgct
     8041 gaaccagact ttcatcaaca ttcaatggct cacgctcggt gacttccaga acaaggttta
     8101 aatcgcggcg ggtaaaactg tcacggaaat tgaaacactc gtcgacaaac gtcggcgaaa
     8161 taatatgcga agcgctaaaa ttaattccaa tatgaaaacc ttccggcaat ttactcgcga
     8221 tagcgttcat ctgtctggca acctgattca tcaggctttg cgtaagcgga acgattaatc
     8281 cggatttttc agcaagtgga ataaatgccg cgggtgatat atatccaccg tgaggttgtt
     8341 tccagcgggc taacacctca actccccgca atgtcccttc ccgaccattt accacaggtt
     8401 ggtaaaaagg gacgacctcc ccattattta ttgcccgtcg caggatctct tcaggcgttg
     8461 cgcttttatt gaaatatcta tcaagcagga aggcagcggc gcaggcgatt aacagaataa
     8521 atattaaaat cccaaagcct ttatcgataa gtctgtagaa gctaaagagt ggcggcgggt
     8581 taaagataat agtgaaatga tattttgtgg agtgaacctt ctcggtatag ggataatgtc
     8641 cattaagcgt catcacatca cccgttggtc caataatgtc atccgccacg cgtaatacat
     8701 accttacccc tttcaagggt acattaagtg ccccacgaat atgctgatcg cttatcgtaa
     8761 ccaaaatgcg cgtatctgca aattggttct gatagagcag gataggtaat ctattaacgg
     8821 tgtcgattgc tggagccaac agtaaattac tgtccgggaa aacaggaata cgagacaaca
     8881 ggacccgatt cccaggcagg gatgtacacc agactattcc ctgtttgaga atgatgattg
     8941 tgcgcaggtg aggtttcaga gctgcttcag tgccaagctg atattgctcc tcgaggttgc
     9001 attccttccc ggcaatgtta accgctgttt gagtcgcttg tgacgcttct tcaagtatat
     9061 gattgatatt gttggcggca tatctcgctc ccgccaggta ctctgctttt gcggaatacc
     9121 agagctggat gttcaaaata gcgacaccgg taataaacaa gcatatcgca acgaggatgg
     9181 gtatgagtgt attgcgcatt tgcttgtccc ggccaaaggg tttaatacat gaaaatgcca
     9241 ttcattcgct cgcgctggca tctacagaga gcatggtaga gagcataaga aatatccacg
     9301 ccgaagataa aattttctcc atcttgatct aacccattaa ttattgagca taatgtaggc
     9361 agtacaaaat aagttaggcg ggatatcagg cgtcaagaat ggagagcgag aactctccat
     9421 tcttgacacc tgatattgcg gacataataa gaaagcataa cgcctgaaat gctcactttg
     9481 catcagcatg gtgatacagc tgatgtttat tctaaaacct tactcaagtt ctaagagagc
     9541 acggattccc tgtcattaat aatgatatcc gttagtgtag tcttatgcat ttcaatgtac
     9601 tgcaatattt gcagatagag atcagaaaga atcacactgg tttcatcttt taaacgcaag
     9661 cgtaaagcct gatcaataat atgactatca acatacccat gcgtcatgaa cacctccctc
     9721 tcgagatttt taaacaaagc acattgaaaa ataaatctct ctggga
//
LOCUS       DBBGLQ010000139         9543 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.139, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000139 DBBGLQ010000000
VERSION     DBBGLQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.139"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..231)
                     /locus_tag="ACIYSB_004689"
     CDS             complement(<1..231)
                     /locus_tag="ACIYSB_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431570.1"
                     /translation="MNSDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFYRSRLAPVTVYASLSPFSDERPSSR"
     gene            complement(228..1127)
                     /locus_tag="ACIYSB_004690"
     CDS             complement(228..1127)
                     /locus_tag="ACIYSB_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGC5431571.1"
                     /translation="MYFSAEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGAVSPENIAFTRWLTYLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVREIITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIHVDF
                     DTPWRQPESDVVAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDELPEVTGPAWIVDKVAHYGG"
     gene            1545..1793
                     /locus_tag="ACIYSB_004691"
     CDS             1545..1793
                     /locus_tag="ACIYSB_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGC5431572.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            1790..2227
                     /gene="umuD"
                     /locus_tag="ACIYSB_004692"
     CDS             1790..2227
                     /gene="umuD"
                     /locus_tag="ACIYSB_004692"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGC5431573.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            2227..2325
                     /locus_tag="ACIYSB_004693"
                     /pseudo
     CDS             2227..2325
                     /locus_tag="ACIYSB_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2426..3955
                     /locus_tag="ACIYSB_004694"
     CDS             2426..3955
                     /locus_tag="ACIYSB_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5431574.1"
                     /translation="MAIVYEIKTTEIKPFTYRTPLITPDENGELSIKYSRQQPKHIKK
                     VVLLNLVGRNTNGDIVSYEPMEQVNRFLLAHHLNDNKQESEQYSKGLVHYFSFLLELQ
                     RLWDSEYDEDLYEEFIDLPRPSWDKFPFRKSDKATYQYREALIKAVLEPDTPNHAIAR
                     TTAIAYMGAVVKFYSFHIRNGYKFNNPPFEHEVVSIQYQGGSTSIGAYLSKDIHTTDL
                     RLNLGKSKRNDGGALSSARRDLKPLTNKEWLAVEDILTNTRRVIKKVAGETTTSNLSI
                     EYCLFFLVARYTGLRKEEVASLHKGQVAKPGEDKKAMKLGVGGQYGSLTKTAGSGNKS
                     RQTIIPTRIMHLLYEYTRSDRYKKRISKFKEHCELKRQKGELGYFDGEDGVDDTKEYL
                     FISQTGVPLFTKLPEANARWNEIRATVNILSGFNLTATIHNLRATFAVSLFRLLLRRV
                     TPDKALALVSECLGHGEESVTLIYLKIAQDEPTGDEIYEDILEFIGAFEDADISGMES
                     Q"
     gene            3955..5805
                     /locus_tag="ACIYSB_004695"
     CDS             3955..5805
                     /locus_tag="ACIYSB_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431575.1"
                     /translation="MPKHNTRKRKYLLPGKNLIKGKAEVKTLHLADMVICVNGSILRF
                     ERFAFKSCPVLFRGFRKVETSQFTDMKRSSFVRQIYSLLSENVTSTTASRYETLIKYV
                     RWVDDSNDTELIDKDMFHWELIDGFMTWCGRQNSKGLLSRPVWGRHRTNISWLLKQLN
                     RTQDTKRLPKISNVSGHTTPHKSLDIERELKPITKRLFNSYFKLLEHYNAGTMPEKHP
                     LYDKELLELIAQKKGIKGSDRVAHFAAFSRAVQPSSGHKNNPITKIAMMLCYLFTGMN
                     TTPLAKMKISDVNFKEVQGGKYILDSVKGRAQHQEQDNALGFSKHAKDFIESWLNVAK
                     SMANGDSNSPLFPYYSKDGQIKLYSEIGSSPHASINKLLTRLDFPKITPSILRKTKMD
                     TLFQATESVYLVAMSANNSIKVVSSQYLHGTSGEHEKKLNAAMDAKFSNAKGVDINTA
                     VEEAKYKHSNILDHYEYEALREGSNRSHEARTPTGIRCNDNKQGAVKTINKMLERIGV
                     EMNSDEEACTSFLDCFECEHHSLVSDVTDIWLMMSFKETLQELEVTPAINSMPPKKMN
                     TLLNTIETILGGFRDKSPENYKQAYELQKQTSHPLYSNIYSLNDLHEVFS"
     gene            5802..7793
                     /locus_tag="ACIYSB_004696"
     CDS             5802..7793
                     /locus_tag="ACIYSB_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431576.1"
                     /translation="MISVLKHNLASVPDYDENIPVSSYSDGSVESYIFDKKWKFSGAI
                     QVGVGGLTDISFSRIDMKYRKYIQSTLAYIMDEYKQKNKTHPTIGQLQHWKNGLKYIV
                     QSLGECNWNLLSDDRIFKQFKKSFSELAIKRSLSQTLINNVVTTFGLLNSLNLCHRIV
                     TIKSLNIQSIKPTKQYIAIPTNIYQQILNDAISTVESLHPHRHEISALMNRIQTIYDE
                     EKNRKDRSSSLGQINDRTRNRIRKIGHNIPDFKLTRDGTNISNILTPCAIVLLAFSGV
                     RVGELNSFTKDSYEEREGEHGDNICFLKGETTKTVNGIPKKEVWQTHRISKDALELAY
                     DCTAYLRTTYIDDVNKQYTEQKINLDEYKKTLRVLESVFLPVTLGGSKKTNYSSTAAR
                     SKNLKSYLEQLNIKATKEDVDSFNTLNPSWHGELKEGQFLPKISAHDFRRTFAVFFKR
                     YGFGTTSTIKFQYKHRNINMSEYYGKNAQLQYFEDVLLDSDLIQLLHEEGVNIAVDMF
                     DEIYNDSETLSGLGGERIAKDKFEKLRSGDKVYMTRTEIESLVRNGTLSIVKLPTGGY
                     CTNSSCSRVCGIGEFSAEIKPCEHQIITDKEAKRTQRQNKRLINTFRDLNNGDPMMKS
                     ILIGVKQKIKINESLIINHGIKVTAFEDNIKGIIATSRG"
     gene            7795..8235
                     /locus_tag="ACIYSB_004697"
     CDS             7795..8235
                     /locus_tag="ACIYSB_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431577.1"
                     /translation="MRKSGKALARLRAALERLISGKPQNVSPSGKLTLNKINNEAGLG
                     NSYIHKFKDFIENEANPAIESFNANYDPVKAKLLQNKQNLTEKEKHKARMKKEVKLKE
                     QYRQERDDLKTINKELETQISSLMFRLYELQEQLNVQNVVKISQ"
     gene            complement(8491..9450)
                     /locus_tag="ACIYSB_004698"
     CDS             complement(8491..9450)
                     /locus_tag="ACIYSB_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HGC5431578.1"
                     /translation="MKLYVIGNGFDVHHGLDTRYTSFGLYLKNNYWETYELLLDYYGF
                     ADLDPDFPTTMSDPLWSEFETSMSLLDKDSVLEANMDAMPNYSSDDFRDRDRYTLEIE
                     MERILGLLTTELYKAFKEFILAVQFPQFDHSRSVNIDRDAVYLTFNYTDTLSQYYAIP
                     DKNVLFIHGKADEHIDELILGHGVDPENFKEKPAEPPSGLSDEDLERWMEYQSDQYDY
                     SFERGKDAVNQYFSATFKGTDQIIKNNEDFFAKLGNIDEVYVLGHSLADVDLPYFKKL
                     EQSVRPDAKWVVTFYDPDDEKVHGETLTGLGIANVAVVRMEQI"
BASE COUNT         3077 a         2068 c         1981 g         2417 t
ORIGIN      
        1 gcggcttgaa gggcgttcat cagaaaaggg cgacaggctg gcgtacactg tcaccggtgc
       61 cagccgggag cggtaaaagc gcccgtcccg tgcccaccgg acgcgggaca ctgaccacag
      121 gcagcgttta tgccgcgccg cgtcagccgg cgcagtaaaa agaggagcac ggcgacatcc
      181 tttaccggaa aagaaacggg cggttgcccg cccgtctccg tcagaattca tccaccgtaa
      241 tgcgccacct tgtcgacaat ccacgcaggt ccggtgactt ccggcagctc atcatctgtc
      301 ggggaagacc attccagttc cccccacagg acatcaacaa gctctccacg ctcatatcgc
      361 tgccagccac agaaattgca gccctgctcg gcataccagt gctccagtgt gcaactgaaa
      421 cggcggctta attctgcaac aacgtcgctt tccggctgac gccacggcgt gtcgaaatcc
      481 acatgaatga agttttctcc ctgactgctg atattcaggt catacccaca aggccacttc
      541 acgccatacc gttctgtata ccagtgatat gcagaaggaa caccgttcag aacaccacca
      601 ttaaagccat tcacttcgac atccaggcgg gtcggcagaa ccgtcagcag gtcaaacggc
      661 atggtttttt ccggaactac gttgtcacac agacggttcc accacaccga tacatcctcg
      721 ttaccccaga tgtcacacca gtcacctctt tttgcggtga aatgcacagt gatgatttcc
      781 ctgacatcat ccggtaatcc ttcccagcga cgctgtccag taccgctctg cagccagagt
      841 tcatgcagca tcaggcagtt ttgctcatcc agcagtacac cgtcctgcag gtatgtcagc
      901 cagcgggtga acgcgatatt ctccggcgat acagcaccac gtccggcagc cgtcagtccg
      961 gggcacggtt caaactgcac atcttcagtg gtctgcagaa gtccggcact tccggccaga
     1021 aacagctgaa taccttcatt tgtggcgcgg cgataaagcg gtgtgactgc accgctggcc
     1081 agtcgtttaa tctcagcaat ctgtgccggt tcggcagaaa aatacatacg attcgagcac
     1141 cagtttggca tgggaattct cctctcacat gcggcctcca tcaggccgtg ccgcacctgt
     1201 aaccgggcaa ccgcatacga atgcaggtgc ggccccgaag ccgcagcgcg tggaggagga
     1261 acgacgaaac gtgaccgggt gaggaggccg gcgacagcga cacacttgca tcggatgcag
     1321 cccggttaac gtgccggcac ggcctgggta accaggtatt ttattcacag aaccatgcgc
     1381 aaaatgctgt ggataagctg gacacagcag tccacagcag gcatacagcc acactctcag
     1441 agaattgagc gctttttagt cacaataatg acatttgact gtgtgtggat tcagttatta
     1501 gaatactgta tatgcataca gcaaaagaaa agggagatga gaacatgatc cgcattgaaa
     1561 ttcttttcga ccgccagagc acaaaaaacc tcaaatcagg cacacttcag gcgctacaga
     1621 atgaaatcga acaacgtctg aaaccacact acccggagat ctggctgcgt atagaccagg
     1681 gcagcgcccc gtctgtatct gtcacaggag cccgcaacga caaggataaa gaacgtatcc
     1741 tgtctctgct ggaagaaatc tggcaggacg acagctggct gcctgcagca tgagtaccgt
     1801 ttatcaccgt ccggctgacc cttcaggcga tgacagttac gtgcgaccgt tgtttgccga
     1861 tcgttgccag gccggttttc cttcgcctgc cactgattat gctgagcagg aactggatct
     1921 gaacagctat tgcatcagca gacctgcagc caccttcttt ctgcgcgcca gcggtgaatc
     1981 gatgaaccag gctggcgtgc agaatggtga tctgctggta gtggacaggg ccgaaaaacc
     2041 acaacacggg gacatcgtta tcgctgagat cgacggtgag ttcaccgtca aacgactgct
     2101 gttgcgccca cgcccggcac tggagccggt ttcagacagc ccggagttcc gcacactgta
     2161 tccggaaaac atctgtattt ttggtgttgt cactcacgtg atacacagga cgcgggagtt
     2221 acgctgatgt ttgcactggc tgatatcaac agtttctacg cctcatgtga aaaagttttc
     2281 cgcccggacc ttcgcaacga accggtcatc gtactcagca ataaccaccc atacactttt
     2341 acttccactc tctaactata cacgtttgac aatgtgagaa tgtccactat tgtgtttaca
     2401 gactgaatga attggatatc accacatggc aatcgtatac gagatcaaaa caacagaaat
     2461 aaaaccattc acgtatagaa cccctctaat tacgcctgat gaaaatggtg aactatctat
     2521 taaatactct cgacaacaac ctaagcatat taaaaaggtt gttttgttga atttggttgg
     2581 tagaaacact aatggcgata tagtctcata cgaaccaatg gaacaagtta atcgcttttt
     2641 actagcacac cacctaaatg acaataagca agagtcagaa caatacagta aaggcttagt
     2701 tcactacttt tcattcttat tagaattaca gcgcttgtgg gactctgaat acgacgaaga
     2761 cctttacgaa gaatttattg atctgcctag acctagctgg gataaattcc catttagaaa
     2821 aagtgataaa gctacgtacc aataccgaga ggctctaata aaggctgtac ttgagcctga
     2881 caccccaaac catgcgatag ccagaactac agcaattgca tatatgggtg ctgtagttaa
     2941 gttttactct ttccacatca gaaatggcta caagtttaac aatcctccct ttgagcacga
     3001 ggttgtttct atacaatatc agggaggcag tacaagcatc ggtgcctact tgagtaaaga
     3061 tatacacaca acagatctgc ggttgaattt aggaaaatct aaacgtaatg acggtggcgc
     3121 actatcatca gctagacgag atttaaaacc cctaacaaat aaagaatggc ttgccgtaga
     3181 ggatattctc accaatacac gtcgagttat caaaaaagtt gctggtgaaa caactacttc
     3241 caacttatca attgaatatt gcctgttctt tcttgtcgca cgatacacag ggcttagaaa
     3301 ggaagaagtg gctagccttc acaaaggtca agtggcaaag cctggtgaag ataagaaagc
     3361 aatgaagctt ggtgttggcg gtcaatacgg ctcattaaca aaaaccgctg gatcaggtaa
     3421 taaatctagg caaacaatca tccccacccg aattatgcac cttttgtacg aatacactag
     3481 aagtgataga tataagaagc gtatctcgaa attcaaagaa cattgtgaac ttaagcgtca
     3541 aaaaggcgaa ctaggatact ttgatgggga ggatggagta gatgatacaa aagagtattt
     3601 attcattagc caaactggtg tcccactttt cactaaactt cccgaggcca acgccagatg
     3661 gaatgaaatt agagccactg taaatatatt gtctgggttt aacctaacag caactataca
     3721 caatcttaga gctacatttg cggtatcact ttttaggctc cttttgaggc gtgtaactcc
     3781 tgacaaagca cttgctttag tgagtgagtg ccttgggcac ggtgaagagt ccgtaacgtt
     3841 aatatacctt aaaatcgctc aagacgaacc tacaggcgat gaaatttatg aagatatcct
     3901 tgaatttata ggtgcatttg aagatgctga tataagtggt atggagagcc aataatgcca
     3961 aaacataata ccagaaagag aaaatatcta cttccaggta aaaacttaat aaaaggcaag
     4021 gccgaagtta aaaccttaca tttagccgat atggttatat gtgtaaacgg cagtattctt
     4081 cgttttgaaa ggtttgcttt taaatcctgt ccagtattgt ttcgaggttt cagaaaggtt
     4141 gaaactagtc agttcactga catgaaacga agcagttttg tgcgtcaaat atactctttg
     4201 ctaagtgaga acgtcacctc taccacagcc tctagatatg aaacgctaat aaaatacgtt
     4261 aggtgggtgg atgatagtaa cgacactgaa ttaatcgata aagacatgtt tcactgggaa
     4321 ctaattgatg gattcatgac atggtgtggg aggcaaaaca gtaagggatt actttctcga
     4381 cctgtctggg ggaggcacag aacaaatatt tcatggcttt taaagcaatt aaataggacg
     4441 caggatacca aaagacttcc taaaatatca aatgtttcag gacacacgac accccacaaa
     4501 agtcttgata ttgagagaga attaaaaccg atcacaaaaa ggttgtttaa ctcgtacttt
     4561 aagcttttag aacactataa tgctggcaca atgcctgaaa aacatccgct ttatgataaa
     4621 gaattgctag aactaatagc ccaaaaaaaa gggataaaag gcagtgacag agttgcccat
     4681 tttgctgcgt tttcaagagc agtacaaccc agctcagggc ataaaaacaa ccccatcaca
     4741 aaaatagcaa tgatgctgtg ctatttgttt acgggaatga atacaacgcc tttagctaag
     4801 atgaaaattt cagacgttaa tttcaaagag gtgcagggtg gaaagtacat tctggattcc
     4861 gtgaaaggta gagctcaaca ccaagagcaa gataacgcac ttggtttcag taaacacgca
     4921 aaggacttca ttgaaagctg gttaaatgta gctaaaagca tggcaaatgg tgatagcaac
     4981 agccctctct tcccctacta tagtaaagat ggtcagataa agctttactc tgaaataggt
     5041 agttcccccc atgcaagcat caacaaacta ttgaccagac tggacttccc taaaataacc
     5101 ccttcaatac tcagaaaaac caaaatggac actctgtttc aagcaacaga gtctgtttat
     5161 cttgttgcaa tgtcggcaaa caacagcatt aaagttgttt catcacagta tttacatggg
     5221 acatctggag agcacgaaaa aaaattaaat gcggcaatgg atgctaaatt ttccaacgct
     5281 aaaggcgttg atatcaacac cgcagttgaa gaggctaaat acaaacattc aaacattctt
     5341 gatcattatg agtatgaagc actgagagag ggctctaaca ggtctcatga agcaagaacc
     5401 cctacaggga tacgttgtaa tgataataaa caaggtgctg taaaaaccat aaataaaatg
     5461 ctagagcgta tcggagttga aatgaattct gatgaagaag cttgcaccag ctttcttgat
     5521 tgctttgagt gtgagcacca ttcattggtc tctgatgtaa cagatatatg gctaatgatg
     5581 tcattcaagg aaacattaca agagcttgaa gtaacacctg caattaactc aatgccacct
     5641 aagaaaatga atacactatt aaacaccatt gaaactattc taggaggatt tagagataaa
     5701 tctccagaaa attataagca agcctatgag ctacaaaaac aaacatcaca ccccttgtat
     5761 tcgaatatct acagtctaaa tgatctgcat gaggtgtttt catgatctca gtattaaaac
     5821 ataatttagc ttcagtacca gattatgacg aaaacatacc agtaagtagt tacagtgatg
     5881 gcagtgttga aagctacatt tttgataaaa agtggaagtt tagcggcgct attcaagtag
     5941 gcgttggtgg tctaacagat atctcttttt cacggattga catgaaatac cgcaagtaca
     6001 ttcagtccac actcgcatac atcatggatg aatataaaca aaagaacaaa acgcatccaa
     6061 ctatcggaca acttcaacac tggaagaatg ggctgaaata tattgttcaa agccttggcg
     6121 agtgtaactg gaaccttcta tcagacgaca gaatattcaa gcaattcaag aaatctttca
     6181 gtgagctagc aattaaaagg agcctttctc aaacactaat caacaatgta gtaaccactt
     6241 ttggtctgct aaatagcctg aacctgtgcc ataggattgt aacgataaaa agcttaaaca
     6301 tacaaagtat aaagccaacc aagcaataca ttgcaattcc aacgaatatc taccaacaaa
     6361 tacttaatga tgctatctca acagtagagt cactgcaccc ccatcgtcat gaaatatcgg
     6421 ctcttatgaa ccgaattcag actatatacg acgaagaaaa aaatcgaaaa gacagatcgt
     6481 cttctctagg ccagataaac gacaggactc gtaatagaat tcggaagata ggtcataaca
     6541 ttcctgattt taagcttact cgtgacggca caaacatttc taatattctg actccctgtg
     6601 cgatagtttt actggcattt agcggtgtaa gagttggaga gttaaatagc tttactaaag
     6661 atagttacga agaaagggaa ggagaacacg gcgataacat atgctttttg aaaggtgaaa
     6721 ctactaaaac agtcaatggt attcctaaaa aagaagtatg gcaaacacac cgcatatcta
     6781 aagatgcact tgagttagcc tatgactgca cagcatatct tcgcacaacc tacatagatg
     6841 atgtgaacaa gcaatacact gaacaaaaga tcaatttaga tgagtacaaa aaaaccttac
     6901 gggtgctaga gtctgtattt ctgccagtaa cactaggagg ctctaaaaag accaactaca
     6961 gctcaacggc tgcacgctca aaaaacctca agtcttattt ggaacaacta aatattaaag
     7021 caacaaaaga agatgttgat tcattcaata cccttaaccc ttcatggcac ggagaactca
     7081 aagaaggtca attcttacct aagatcagtg ctcatgactt tagacgaaca ttcgcagtat
     7141 tctttaagcg ttatggattt ggaacaacgt caacaatcaa gtttcaatat aaacacagaa
     7201 atataaacat gtccgaatat tacggcaaaa acgcccaact tcagtacttc gaagatgtct
     7261 tgttagactc tgacttaatc caactattgc atgaagaagg ggttaacatt gcagtcgata
     7321 tgtttgatga aatatacaac gattcagaaa cgctaagtgg gctaggtgga gaacgaatag
     7381 ccaaggataa gtttgaaaaa ctacgctctg gagacaaggt atatatgacg cggactgaaa
     7441 ttgagtccct agttcgcaat ggcacgcttt ctattgtcaa gctacctaca ggcggatact
     7501 gcactaattc gtcttgcagt agagtgtgtg gtattggcga gttctccgct gaaattaaac
     7561 catgcgaaca tcagataatc acagacaagg aggccaaaag aactcaacgt caaaataagc
     7621 ggctaataaa tacatttcga gatttgaata atggcgaccc tatgatgaaa agtatactta
     7681 tcggagttaa gcaaaagata aaaataaatg agtcattgat tatcaaccat ggaattaaag
     7741 tcacagcctt tgaggataat atcaaaggca taatcgcaac cagtagaggt taatatgaga
     7801 aaatcaggaa aagcattagc gagattgcga gcagctttag aacgcctaat ttcgggcaag
     7861 ccacaaaatg tttcaccttc aggtaaatta acccttaata aaataaataa cgaagctgga
     7921 cttggtaaca gttacataca caaatttaaa gattttattg aaaatgaagc aaacccagcc
     7981 attgagtctt ttaacgctaa ttacgatccc gtcaaagcga aactattaca aaacaagcag
     8041 aatctaactg agaaagaaaa gcataaagcc agaatgaaga aagaagtcaa acttaaagag
     8101 caataccgcc aagagcgaga tgatctaaaa actataaaca aagaattaga aacacaaatt
     8161 agttctttga tgttcaggct gtatgaatta caagaacaac ttaatgtaca aaatgttgtg
     8221 aagatctctc aataactatt gccttaatgt cggtggacaa taagatcgtg actctgacac
     8281 caatgtctga aacttttaca ttaaagatta aaacttttcg gggttcattt ggctggttct
     8341 tttgtgtttg gactgccatt ttataaaaca cgtttctgga caatttcgag ttagcgctgg
     8401 tgttcaaaat tgaattcttc gcataagcaa aatttagaaa aaacaatgtt agaatccacc
     8461 acaatcgcta atgatatttc aggcttttgc ttagatctgt tccattctga caactgcaac
     8521 gttggctatt ccaagtcctg ttagagtttc accgtgtact ttctcatcat cagggtcata
     8581 aaaagttaca acccatttag catcaggtct gactgattgc tccaacttct tgaagtaggg
     8641 taaatctacg tcagctaatg agtgaccgag aacatatact tcatcaatat tgccgagctt
     8701 tgcaaagaag tcttcattgt tttttatgat ctggtctgtt cccttgaaag ttgctgagaa
     8761 atactgattg accgcatcct taccacgctc gaatgagtag tcatattgat cagattgata
     8821 ttccatccaa cgttctaaat cttcatcact caatcctgat ggtggttcgg caggtttctc
     8881 tttgaaattt tcaggatcaa caccatgacc gagaattaac tcatcaatat gttcatcggc
     8941 tttcccgtga atgaatagca cgttcttatc ggggattgcg tagtattgag aaagcgtatc
     9001 tgtgtagttg aatgtgaggt aaacagcgtc tctatcaatg tttactgatc ggctgtgatc
     9061 aaactgagga aactgtacag ccaaaatgaa ctctttgaat gctttataca gttcagtggt
     9121 tagaaggcca agtattcgct ccatctcaat ttcaagcgtg tatcggtctc tgtctcgaaa
     9181 gtcatctgaa gaatagtttg gcattgcatc catgtttgct tccaaaactg aatctttatc
     9241 aagcagtgac atactcgttt caaattcaga ccataaaggg tctgacattg ttgttggaaa
     9301 atctgggtct aagtcagcaa acccgtaata gtcgagcaat agctcataag tttcccagta
     9361 gttatttttg agataaagcc caaaagatgt atacctagtg tcaaggccat gatgaacatc
     9421 aaagccatta cctattacat atagcttcat gtcattcacc gttgatatct agttctttga
     9481 agctaatgac ttcggagtca ttatcagtca gctcaaaata tgtatcttgg aagtaaaact
     9541 ctt
//
LOCUS       DBBGLQ010000140         9424 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.140, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000140 DBBGLQ010000000
VERSION     DBBGLQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.140"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(154..492)
                     /locus_tag="ACIYSB_004699"
     CDS             complement(154..492)
                     /locus_tag="ACIYSB_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type lysozyme inhibitor"
                     /protein_id="HGC5431579.1"
                     /translation="MKKYSSPVLALILVSNSMVSSAATKEISKTAYVCNGNKTMEVVY
                     INTTAGNSYAVINQMDEMIPMQLMKMASGANYEAINKRYSYKLYTKGKKANLVEGNDN
                     PVLSNCSSAE"
     gene            complement(997..1686)
                     /locus_tag="ACIYSB_004700"
     CDS             complement(997..1686)
                     /locus_tag="ACIYSB_004700"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003825705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGC5431580.1"
                     /translation="MKTVLDSLKGKLIVSCQALENEPLHSPFIMSRMALAAAQGGAAG
                     IRANSVADISAIKEIVSLPMIGIIKRDYPGSEVFITATLREVDELMTVEPEIIALDAT
                     ARPRPGGQTLEELVTQIRARYPSILLMADIATVEEAVTAERLGFDCVGTTLYGYTAET
                     ARHVLAENDCGFLREVLKAVSVPVVAEGNVDTPECAARCLTLGAHTVVVGGAITRPQQ
                     ITTRFIAAIAS"
     gene            complement(1686..3242)
                     /locus_tag="ACIYSB_004701"
     CDS             complement(1686..3242)
                     /locus_tag="ACIYSB_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/glucose-specific PTS transporter subunit
                     IIC"
                     /protein_id="HGC5431581.1"
                     /translation="MMQMFSGASSGGWFEKAQRFGKSFMLPIAVLPAAGLLLGIGGAL
                     SNPNTLTAYPFLDADWLQAIFTIMTSAGSIVFANLSVLFAVGVAVGLAKSDKGTAGLA
                     SLLAFLVMNATINALLTLNGTLAHKNPASVGQGMTLGIQTLETGVFGGVVIGLVTCTL
                     HSRFNKVSLPQFLGFFSGSRFVPIISSLAAIVVGAVMTVVWPHFQKLIFGLGDLVDAT
                     GYLGTLLYGFILRMLGPLGLHHIFYLPFWTTALGGSEIVNGQLVEGTQRIFFAQLADP
                     NTQQFYAGTARFMSGRFITMMFGLLGACLAMYHTAKPENRKVVAGLLLSAALTSFLTG
                     ITEPVEFSFLFVAPVLYVIHAFFDGLAFMVAHILHITIGQTFSGGLIDFLLFGVLQGE
                     AKTNWMYVPIVGIPWFFLYYFTFRYLINRFGWLTPGRENVTQVESGQPQSERAAAVIA
                     GLGGKENLEEVDCCATRLRVTVKESSKVDEAALKVAGARGVIIRGNGVQVIYGPHVTI
                     IKNEVEEILS"
     gene            3408..4232
                     /locus_tag="ACIYSB_004702"
     CDS             3408..4232
                     /locus_tag="ACIYSB_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGC5431582.1"
                     /translation="MSENENLLLRLRQSVDGYSRTQQKLGEFVLSDPAKVVYLTITEL
                     ARESGTSEASVTRLCRTLGCKGYNEFKMALALDLRQVQFVEHGGDEIDNVVNESVQAI
                     QDTAKLLDRTLLEAAALALHQAQSVQIYGVAASAILGEYLHYKLLRLGKPAQLFSDMH
                     RAAMNATTLSKNTFVVAISSSGSTRDLLHVVKLARKQGVRVLALSNTPRSPLASLSDI
                     QLVAAKPEGPLSAGALTAKVGVMLLVELLTTSLIALDDQYSEVSQKTASATLPLLL"
     gene            complement(4389..5732)
                     /locus_tag="ACIYSB_004703"
     CDS             complement(4389..5732)
                     /locus_tag="ACIYSB_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016929550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HGC5431583.1"
                     /translation="MHNSKIRLAILVGCMALSANLWAKAQPVQATLAGHAILPAKSAV
                     STPKDAPGDLQQSGKYTSGKRVTELGSVAGKSAERLTGLSLPINGQPLQGHSGIKHMP
                     DGTYWVLTDNGFGSKANSPDAMLYLNQYKIDFKDGSVVPLKTLFLHDPDKKVPFHIIN
                     ESTELRYLTGSDFDPESFQFSDDALWIGDEFGPYLIKTDLNGKVLAVFDTEVDGKVVK
                     SPDNPTLTLPSAPDGKLNFQVARSKGFEGMAISPDGSKLYPLLEGALWDGEQFENVDG
                     KRYLRVLEFDAKQQAWTGRSWQYVLEDNQNAIGDFNMIDDTHGLVIERDNGEGTADKA
                     CVAGAPTNNCFSQVARFKRVYKIAFSDTNVGKPVEKLGYIDLMKIQDPNKLARKPLND
                     GVLTFPFFTIENVDVVDTNHIIVGNDNNFPFSSSRWPNMADDNEFILLDVKDFLK"
     gene            complement(6091..7088)
                     /locus_tag="ACIYSB_004704"
                     /pseudo
     CDS             complement(6091..7088)
                     /locus_tag="ACIYSB_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(7227..8543)
                     /gene="ugpB"
                     /locus_tag="ACIYSB_004705"
     CDS             complement(7227..8543)
                     /gene="ugpB"
                     /locus_tag="ACIYSB_004705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGC5431584.1"
                     /translation="MKPLHYTASALVLGLTLMGNAQAVTTIPFWHSMEGELGKEVNSL
                     VQRFNAENPDYKIIPTYKGNYEESLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGINFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAS
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPSLPFTKGLRLGN
                     MPQIRAIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            8744..8899
                     /locus_tag="ACIYSB_004706"
     CDS             8744..8899
                     /locus_tag="ACIYSB_004706"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGC5431585.1"
                     /translation="MRKTCPAVIVSNDAANRNLARVVVVPLTSNTERVYPSEAVVTVG
                     VRAAKRL"
     gene            8908..9015
                     /locus_tag="ACIYSB_004707"
     CDS             8908..9015
                     /locus_tag="ACIYSB_004707"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein"
                     /protein_id="HGC5431586.1"
                     /translation="MAADKSCLKTHIGSLSKADMLSVEDAIKIHLGLLR"
BASE COUNT         2360 a         2416 c         2273 g         2375 t
ORIGIN      
        1 atagccatag tgaaataaaa agctgtctga attaaattat tttgcacaca tgttttaatt
       61 ctgcactgaa ttcagaatgg gtaatcagtg accgtcctga cggtcacttt aaagaaaagg
      121 catttacaca gaattactga caggctctgg ctgtcattcg gccgatgaac agttgcttaa
      181 gacaggatta tcattccctt ccaccagatt ggctttttta ccttttgtgt acagtttata
      241 tgagtatctt ttattgatgg cttcgtaatt ggctccggag gccattttca ttagctgcat
      301 aggtatcatc tcatccatct gattaatcac tgcatatgag ttcccagcag tggtgttgat
      361 atagaccacc tccattgtct tatttccatt acagacataa gctgtttttg agatttcttt
      421 tgtggcagcg gaggatacca tactgttgct aacaagaatg agtgcaagga cgggagaaga
      481 gtattttttc ataataactc cttctgtagg ttaaaaaaac aggtgtttga tcagaattat
      541 attaataatt cattcatcag aaaaaaaggt ttttgttata gcaataataa tgagcacaat
      601 tgcttgtgaa actggtgcca ttttacggtt gcttaggagt gctgaaactg tgcctgttag
      661 ggcacagctg gtgtggtggt ctcagtacgg aacaatttga aaaccagaac atcacttagg
      721 cttgtgtcca cattacatgg ggtaggatca gtattcctct atcaggggta actcgaccag
      781 gctggctgag ctctcgtttt actgcgagta tctatgccca ttcagccagt gtcagcgcga
      841 gagtgttaaa ttccgagcga ttatccatgc gcagcatgac cggataacca tgatttagcc
      901 cctgccgagc taatcgggaa ctgcatagca ctctcaatat ggaataagcg ataattatcg
      961 cttattccca gcaaaatatc atgcctcatc ggtgctttac gaggcgatcg ctgcaataaa
     1021 gcgtgtcgtt atctgctgcg gacgagtgat tgccccccca accaccacgg tatgagcgcc
     1081 taatgtcagg caacgtgcgg cgcactccgg ggtgtcgaca ttcccttccg ccacgaccgg
     1141 aacgctgacc gctttcagga cctcacgtaa gaatccacag tcattttccg ccagcacatg
     1201 cctggcagtt tctgcggtat agccgtagag cgtagtgccc acacagtcaa aacccagacg
     1261 ttcagctgtc actgcctctt ctactgttgc tatatctgcc atcagaagta tggacgggta
     1321 gcgagcgcgg atctgcgtga ccagttcttc cagcgtctga ccaccagggc gcgggcgcgc
     1381 agtagcatcc agggcaataa tttccggctc aacggtcatc agctcatcca cttcccggag
     1441 agtggccgtg ataaacacct cactgccagg gtaatcgcgt ttgataatcc ctatcatcgg
     1501 aagggagaca atctctttga tagcactaat atccgccacg ctgttggcac ggatacctgc
     1561 agcaccacct tgtgctgccg ccagcgccat acgtgacata ataaacggac tatgtagagg
     1621 ctcattttcc agagcctgac aggagacgat taatttacct ttcagggaat ccagtacagt
     1681 tttcattacg ataaaatctc ttcgacttcg tttttaatga tggtgacatg agggccataa
     1741 atcacctgaa cgccgttgcc gcgaataatc acgccacgcg ctccggccac tttcagcgca
     1801 gcttcatcta ccttactgct ctctttaacc gtcacgcgca ggcgcgtggc gcaacagtcc
     1861 acctcttcca gattctcttt gcctcccagc ccggcaatga ctgccgctgc gcgttcactc
     1921 tgaggctggc cggattcaac ctgggttacg ttctcacgcc ccggcgtcag ccagccaaag
     1981 cggttaatca aatagcggaa agtaaagtag taaaggaaga accacggtat tcccactatg
     2041 gggacataca tccagtttgt tttagcttcc ccctgcaaca cgccgaagag aaggaaatcg
     2101 ataagcccgc ccgaaaaggt ttgcccaata gtgatatgta aaatatgggc gaccataaac
     2161 gccagaccat cgaagaaggc gtgaattacg tataatacgg gcgcgacaaa caggaaggag
     2221 aactcaacag gctcagtgat tcccgtcagg aaagaggtca gggctgccga caaaagtagt
     2281 ccggcgacaa cctttctatt ctccggcttt gcagtatggt acattgccag acatgcgcca
     2341 agcaggccaa acatcatggt gataaagcgc ccggacataa agcgagcggt acccgcgtag
     2401 aactgctgcg tattcggatc ggcaagttgg gcaaagaaaa tccgctgcgt accttcaacc
     2461 aactggccgt tgacgatttc actgccgccc agcgccgtcg tccagaacgg caaataaaag
     2521 atatggtgta aacccagtgg accgagcata cgcaggatga agccgtacaa cagcgtcccc
     2581 agatagcccg tcgcatccac caggtcgccc agtccaaaaa tgagtttctg gaaatgcggc
     2641 cacaccaccg tcataaccgc acccactact atcgcggcaa gggagctgat aatgggtaca
     2701 aaacgagaac cactaaaaaa accgagaaac tgaggtagtg agactttatt gaaacggcta
     2761 tgtaaagtgc aggtgacgag accaatcacc accccaccaa acacaccagt ttccagtgtc
     2821 tgaataccaa gcgtcatccc ctggccaact gaagcggggt tcttgtgcgc cagcgttccg
     2881 ttgagcgtta ataaggcgtt gatggtggca ttcatcacca gaaacgccag caatgacgca
     2941 agcccggcag tgcctttatc cgatttcgcc agccccacgg cgacgccgac tgcaaagagt
     3001 accgacaggt tggcaaacac aatagaacct gcgctggtca tgattgtgaa tattgcctgc
     3061 aaccagtcgg catctaaaaa cggataagcc gtcaacgtgt ttggatttga caacgcgcca
     3121 ccaatcccta ataacagccc tgctgcgggc aataccgcaa tcggcagcat gaaggatttt
     3181 ccgaaccgct gcgctttttc aaaccatccc cccgatgaag cgccgctaaa catttgcatc
     3241 attttatcat ctcctcgatt tttgatgaaa atttttacta taattattct tataaataaa
     3301 aatcaccatc aaaatctaag aagtcgatca tactttttta aaatgtctgg catcaatccc
     3361 ctcctttccg tcacactatc aacagataaa cgcattaagg attttacatg tcagaaaatg
     3421 aaaacctgct gctgagattg cgccagagcg ttgatggata tagccgaaca caacagaagc
     3481 tgggagagtt tgtacttagc gatccggcaa aagttgtcta cctgacgatt accgaacttg
     3541 cgcgtgaaag cggcaccagc gaggctagcg tcacacgtct gtgccgaacg cttgggtgta
     3601 agggctataa cgaattcaaa atggcgctgg cactcgatct ccgacaagta cagtttgtag
     3661 agcacggcgg tgatgagatt gacaatgtgg tcaatgaatc cgtgcaagca atacaggaca
     3721 cagcaaaatt actcgacaga accttgctgg aagcagccgc gcttgcactg catcaggctc
     3781 agtcagtgca gatttatggc gtagcagcca gtgccattct gggggagtat ttacattata
     3841 aactcctgcg tctggggaaa cccgcacaat tgttcagcga tatgcatcgt gctgccatga
     3901 acgccacaac acttagtaaa aacacgttcg tggtggcgat ttccagttct ggatccacgc
     3961 gcgatctgct gcatgtcgta aaactagcgc gtaagcaagg tgtgcgtgta ctggcgctga
     4021 gtaatacacc gcgcagtccg ttagcttcgc tgagtgatat tcaactggtc gcggccaaac
     4081 cggaaggacc cttgagtgcc ggcgcgctga ctgccaaagt gggagtgatg ctgttggtag
     4141 agctactcac cacatcgctg attgcgctgg atgaccagta cagcgaggtg agccagaaaa
     4201 cagccagcgc gaccctaccg ctactgcttt aaagacataa aaaaccgccg aagcgctggg
     4261 tgctccccat gaatcagtga taataaacca acatcatatt ctggtaggtt ctggcgaggt
     4321 gattaaaaac ggtgctcagc accgttcgtt tttaaattag cgaggagtat cgtaagatgc
     4381 tcttccccct acttcagaaa atccttcaca tccagtagaa taaactcgtt gtcatccgcc
     4441 atattaggcc agcggctgga ggaaaacggg aagttattat cgttgcctac gatgatgtgg
     4501 ttagtgtcaa ccacatccac gttctcaatg gtgaagaacg ggaaggtcag cacaccgtcg
     4561 ttcagcggct tgcgtgccag cttgttcgga tcctgaatct tcatcaggtc aatatagccc
     4621 agtttttcga caggttttcc gacgttagtg tccgagaaag caattttata cacccgtttg
     4681 aacctggcta cttgactgaa gcagttgttg gtcggcgcgc cggcgacgca ggctttatcc
     4741 gccgtgcctt cgccgttatc acgctcgatc accagtccgt gggtatcatc tatcatattg
     4801 aagtcgccga tggcgttctg gttgtcttcc agcacgtact gccagctgcg accagtccag
     4861 gcctgctgtt tggcatcgaa ctccagcaca cgcaggtagc gttttccgtc aacgttctcg
     4921 aactgctcac cgtcccacaa tgccccttcc agcagcggat acagcttact gccgtccggc
     4981 gagatggcca tgccttcaaa gcctttggaa cgcgccacct ggaagttcag cttaccgtcc
     5041 ggagcgctag gtagagtcag cgtcgggttg tccggcgatt taaccacctt accgtccact
     5101 tctgtgtcga acaccgccag caccttgccg ttcaggtcgg tcttgatcag gtaaggaccg
     5161 aactcatcac cgatccataa ggcatcgtcg gaaaactgga agctttccgg atcaaaatcg
     5221 ctgccggtca gatagcgtag ttcggtgctc tcgttgatga tgtggaacgg caccttttta
     5281 tccggatcat gcaggaacag cgtcttcagc ggtactaccg agccgtcttt gaaatctatc
     5341 ttgtattggt tgaggtacag catggcgtcc ggggagttgg ctttgctgcc gaagccattg
     5401 tcagtcagca cccaataggt accgtcgggc atgtgtttga tacctgagtg gccctgcaac
     5461 ggctgaccat tgatcggcag gctgagtcct gtcaggcgtt ctgcggattt gcccgccacg
     5521 ctgcccagct cggtgacgcg tttgccgctg gtgtatttgc cgctctgttg caaatcgccc
     5581 ggcgcatcct tcggtgttga aacagcggat tttgccggca gaattgcatg cccggccagc
     5641 gttgcctgca ccggctgcgc tttggcccac aggttggcgc tcagagccat gcagccaacc
     5701 agtatagcca gccggatttt ggagttgtgc atgtcatact ctcctttcgt tgttatcgcc
     5761 cagaagtaaa actaacgtca ttgtagggag cagaaatgac agaaatatta tgattacggt
     5821 agggatagcc gttgccggct tccccgccat gtcaacgccg gtatttatgg tttctgtttg
     5881 cttttctgta aaaataaagg ggagagacga cggtactata aacaaccatg atgaatctca
     5941 tctattcggc ccagatgaaa gacgcgcgtg aggctcttgt tcgttaagcg gatacgtata
     6001 tcctgtacaa taatcagata tacctcacag ggtctaacac tttatcatca acgagttgca
     6061 gatcagatag tcatacggtt gctgcgtgct ttagctggct gaatacagcc tgaaatcatc
     6121 accgtaatga catgatgccc agcacagcct tacctttctg gcggcgatgg ccacaacagc
     6181 gcgtcagtat tctctgcgtt caaccagtga acagacccaa cgactgaatg agtcagtcct
     6241 tttctcagca ccaatcagta ctgaacgagc cccctgaacc agaagcattc gcagatacga
     6301 atcaccggct tttgttatcc tgcccagctt cgacattcag ccgctgctgt attgtgatag
     6361 cgtcagccca gccaggctgc cagttgccgt ccctttttaa aatcatgtgc attaccgata
     6421 caggcaacca gcgcacaggc tgttgtggga ccaactcctt ttagctccat cagctgttta
     6481 atacggatat catcccttgc agcctcatga agacggtggt catgagacga tatgagttca
     6541 ttttactctg acagttcgca caacaaacgg atgaagggtc attccactgc ccctgggcag
     6601 tattccctgc tggattttac gcacgtgcct tatccccaca ggcagcagca gaccaaattc
     6661 actcaacagc ccccggattt cattacgcaa cgccgttcgc tcgcggatga ttctggcccg
     6721 gacccggtat tcttcctgta acgtctgctg ggattccgat atgaaaacaa caaatcgcat
     6781 gccgggcctg ctgacggcct cacagatggc agctgcatta ttagcgtcgt ttttgttacc
     6841 tttgagataa gatttgacaa gtttctacgg aataatgcgc acggtaggcc ccatacggaa
     6901 tgagttcccg tgcccagaat gggaagagcc acagacttcg atgctgatga ggcagggaga
     6961 aagcccggag aaaaacgtca tcatgtgggg agggctcagc gtttttctcc gtaccactat
     7021 tcattgtgat caacgccgtg gatttgaaaa acatttttcg gcaagatcga aaccaatacg
     7081 attaatattc atgactgact tctccaccag ggaactgggt acatctcagt ctggaacgtt
     7141 gatgccgtaa ggaggaagat gtcaatcaca tcggcctaca cgcgatgtta gctcagatag
     7201 gcgaaacgta tccgacatta cactggttaa gacttcgtcg atttctcaaa gcggcgtagt
     7261 aactgattcc cacgctcaac ggcggtatcc agcgcctgct gtggcgtctt cttaccggtc
     7321 cacacgctct caagctcttc atccacaatc gctctgatct gcggcatgtt gcccagacgt
     7381 agtcctttgg tgaacggtaa cgacggtttg ttcagcatct gacgtgtcgc gatatccgcc
     7441 cctgggtttt tctcgtaaaa gccctgctca cgggtcaggt catacgccgc tttggtgatt
     7501 ggcagataac cggttttctg gtgccactcg gcagcatttt ctggcttcgc gaggaagtcg
     7561 aggaacttcg ccacgccggt atacgtttct ttatctttgc cctgcatcac ccacaggctg
     7621 gctccgccga taatggcgtt ttgtggtgca tctttcgcgt cagcgtcgta aggcatcatg
     7681 cctacgccgt agttgaattt ggcgtactcg cgaatgctgg caagagaacc agaagaggca
     7741 gtcgtcattg cgcaatcacc gttatagaac ttctcggtgg attcatcctt acgcccgacg
     7801 tagctgaaat cgcctttctt gttcatctct tcgagcatgg cgatgtgttt cacctgctcc
     7861 ggcttgttga actccagcac cgcatctgtg ccgtcaaagc cgttgttttt gctggcaaac
     7921 ggcagaccgt tccaggcgct aaagttttcc agttggatcc agccctgcca gccgctggcg
     7981 tagccgcact tcataccgga ggctttcagt ttcgcggcat agtccgccag atcctgccag
     8041 gttttcggcg gctgttccgg gtctaatcct gctttcttga aagcatcttt gttgtaatag
     8101 agaacaggag tcgagctgtt gaacggctgg gagagtaagt gtccggtttt actgtcggag
     8161 tagtagcctg aaaccgtcgg cacaaactgc gactcatcaa agttaatccc cgcctcttta
     8221 aacacgtcat acaccggttt aatggctttc gacgccatca tggtggcggt gccaacttca
     8281 taaacctgca aaatagccgg agcgttaccg gtacgaaatg ctgcgatccc cgcacttaaa
     8341 ctctcttcgt agttgccttt ataagtcggt ataattttgt aatccgggtt ttcggcgtta
     8401 aaacgttgga ccagagaatt cacctcttta cccagttccc cttccataga gtgccagaac
     8461 ggaatggtcg tcactgcctg tgcattcccc attaacgtca gtccgagcac cagtgctgaa
     8521 gctgtataat gtaacggttt catcgtttat ctctcttgct tgttgtacct gatgagcgaa
     8581 ttcacacgtt tcatgctcgt gggattaaat atcatgcccg aattgcagaa aaataacttt
     8641 tttattacat gtgttagaca gtaaagtgtt agaaagatga cagagcagtg acggcacagg
     8701 tgagacgtgg tggattgaat ttgatcctgc tgttgacagt gaaatccgca agacatgtcc
     8761 ggcagtgatc gtgagcaatg acgcagcaaa caggaacctc gctcgagtag tcgtcgttcc
     8821 tctgaccagt aacacagagc gagtgtatcc aagtgaggct gttgtgaccg taggtgtcag
     8881 agcagcaaag cgattgtaga gcagattatg gcggcggata agtcctgtct caaaacacat
     8941 attggctcgt tgtccaaagc agatatgttg tctgttgagg acgcaatcaa gatccatctt
     9001 ggtttactgc ggtaacgcca tgtgaatctg tcggctaccg ccattagtga cggaattttc
     9061 tgtggggcag cgtaccaatg gcattcggtt aagttcttgc tggagcatca aaagagttcc
     9121 agcgcgttct gggccgtaca tattttcgtg ggtaacacct ccctggcctt actcgctgag
     9181 tcatacaact gcgatcgatt cagatgccat tttgcaatca gcctctcaga aagagcgcaa
     9241 cagaatgatt ttaaatgttt tcctgttacc cgaatcagta aagacaagta cctcactacg
     9301 agaaacaagc agtttttgaa attaaaattt tgtttatttc tactactacc gatgaaaacg
     9361 ttattatctt aataattaat gtcatttcag gatatccatg taaggttaag taataatata
     9421 tgag
//
LOCUS       DBBGLQ010000141         9400 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.141, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000141 DBBGLQ010000000
VERSION     DBBGLQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.141"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..518)
                     /locus_tag="ACIYSB_004708"
     CDS             complement(<1..518)
                     /locus_tag="ACIYSB_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HGC5431587.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGR"
     gene            742..907
                     /locus_tag="ACIYSB_004709"
                     /pseudo
     CDS             742..907
                     /locus_tag="ACIYSB_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1131..2780
                     /gene="dptF"
                     /locus_tag="ACIYSB_004710"
     CDS             1131..2780
                     /gene="dptF"
                     /locus_tag="ACIYSB_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptF"
                     /protein_id="HGC5431588.1"
                     /translation="MSAITLRKALGVLAKSSSFSVTTVTHRQKDEFDQLKEQLFVKQE
                     IETELQRYLDVAKPGEIIFLCGSSGDGKSEILTRCKSNPRYQQRFSFHLDATHSFAPR
                     QSAIDALNDLFSNHHQYSSPLLIGINTGMLANFAREGAECHLAIRTAIDSFLSADQEE
                     SRPYRSGHCSFFDFEHYPKFQFNEKKQYSSFIKTLLDNLTRNDDSNLFQFIFRHDETV
                     NPELKEVANYKLLCLPGVQDVLITQLFKARLIKDQFVTTRTLLDFLHHLLMGPGYLFD
                     NLFTGAENDLIKKVSDFDPARLHTYEIDQFILRYELGLVDPELDDFLAALAPLHIRFD
                     RQCVNPGDAASLIRLFWLLQDESLGNNYHQKFSVFFNESLFEHYSEIWHLHKNYIADS
                     EQKKALNRFYTSELIAGIQRYANRKAPELSMQKEEFFLGEYGGVKITAPVELKPDWEA
                     IRNKNTAHPTGFDVHLKVGQNSLLPVRIGLNLFELLNKLNNGYRPNKYDKNAIVLLDE
                     IVDLITEQAKSSSEIKFYAGGRRVYRAKADDDMITISGMEG"
     gene            2785..4110
                     /gene="dptG"
                     /locus_tag="ACIYSB_004711"
     CDS             2785..4110
                     /gene="dptG"
                     /locus_tag="ACIYSB_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HGC5431589.1"
                     /translation="MYPIATTLKVGNNQLDSYLPIRNKNNDINWQFVTGLVLSYALKR
                     KIDAYTPSQFRDDCKAHLQELLDEPAFWTVLERMYFSSEDIFRVSPLFLLFHAQFDGE
                     KISGASMADKRLGTLFANLMGDFSLEYPIQDKLNFIEQQMLNKLNEKIKLLGKGPFSE
                     EQPYLPYLVTCFQSDLAFLAEHPQYLLQELTNTLRLYAFSWCAQLALNLDNWQDGEPQ
                     SKSLFFILDTEKASSERDQIKRFGYKWFARQSEKLFPILSALEVLQIKGDKKRPLWQV
                     YQDCLNFSDNSSQLLQDLNNYLQEFIEKRELSASKYTQAVTLEEAFKQLLTVAVEQFQ
                     DKKSDRATVNRKYINELETQICTDFIQVRGRAGKVLVLNQDRLLLLTNLTVGKNDKLR
                     LHELLRGFEQRGFYLDNQSAQTLVAFYERMGNVERMSDSGDAVYVRKTV"
     gene            4091..9145
                     /gene="dptH"
                     /locus_tag="ACIYSB_004712"
     CDS             4091..9145
                     /gene="dptH"
                     /locus_tag="ACIYSB_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptH"
                     /protein_id="HGC5431590.1"
                     /translation="MSVKQYETYLAETFIEWVSGIIQPGERYQFKSPDPDNALQLWQA
                     FVDLAGDNHLEIAPEQRLACLSCNGIQLIPVLHGAAAPAFTENYISHLRDEVAGRSGV
                     FAKTALLIIHNSMLDTLINSTKDVAAPGAIWHPETFSHELEKLITTDNNRSELSRCLL
                     KDQRTTVLDEGATVFGFSSLYRLLDDGNLDFSELSLFKDDELLNFSQKQLHTRLNENR
                     KLFRQIEDSVERYSGQLENVLTEFSAKFIQEHFNDKDDWRELDFAVYLNEKEQNREQK
                     LVLDDITVENGVIWQRAKSASKAGKRDISLLVQVPPEQSQTELEFCFQGNDLQDNQIK
                     IAHHRQLKKERFWRISRAGGKSSRIMASVPFDGNPCFFSLELTNRNNSAEEYKFRLLL
                     VRQGQFWLDDIQHCFRIEPGKPQITLQREDNELRIAESGSQVCILDEENGDIDCQHYA
                     LVNFETLANQSDLIQFKLVSGDSCLAFNIEGPGAEEGLTLPLLFDQSRFNKLFKEDGN
                     ASWNRLKGRIILDNTEHKVVGVRQQLLALEASLVDQRLLGTGDDDSAFALDELVAIHP
                     DLYNAYDQLFLYYQRCGTLPSLVSWSAEYCALVSHIVTTFEQALQQIELSRALTAQEK
                     RLLHLGICNVDSHERLSPLHPLVLAYHLQLVQTICAEQEQYDSASFATLPTITLDRLV
                     VSGLMPFVYHSEHEYAQLQPVEENRFWIDVVPQRQVSHDYVKRLVKDKLNEFTEAYAR
                     LFQSPGNNALIINAINQGTAKELFLGLVEYFKQEKEHAISVHVNCYDERLLPNMFDRF
                     AESGSYEQLKNDLDLNRGAWRAEADMLIDLLRSRLTFSKFVLPSESDKLAYAHLAFFT
                     NTAPVDCRQIRIEDAASGVLCHGLISGEGAETQGDAYFTAFGLRNVDTESYCALRLAR
                     LVGCLWQPARQSNSQYHGQGIGLAVSGNFKQLLNYSYNSALWTTIIDPKVTLDFFTSQ
                     KDVVLIHYSDQYTSCAGYDAVTVTKQVDLFLRLLQTESQSGQSAVDSQHLLAEFNAFN
                     GEWLLKMLRSSEKERKEKYGIIGAYKFVQSMLSESDICWVPLSVAEMIRVSGNVGLKM
                     KESDLSRNLQGYRKGAISDDVLFVGFKENRLYLLPLEVKTGARPDYNYAGQQAAELKR
                     YLQQDILEPHTLASQLYRALFIRQVLMQVEKLQLYGVLDSDKLAPLLDRREWWLTGDY
                     QLGELKDYANGFVVAHVDSGSCFDLSYKETTENILQIEIPYSLLSSLITTREGKLPLA
                     ERYRVPDKYRLKPESDEHPSPSASGVQVTTPPDTRPDIPKPTPEVSTVPLQVLFGHDA
                     TRQTPLFWEPTNTTKFMNTNTGIIGTMGTGKTQFTKSLVTQLMRNQSCNVDGKSIGLL
                     IFDYKSDYVDEAFLKATGGKKYQLSLLPYNPLSLFGDMPMLPRHTAIAFSETMGKAYN
                     LGVKQRMKLVTLIMECYELAGIVPHDRSTWNRVAPTIEDVWQRYLAQEKVEEDSLYAA
                     LYNLAGFQIFETDPEKMTSLYDLVDGVTVIELAGYSSEIQNLVVALTLDLFYAQMQKR
                     GKPVIQGDYRQLTKMILVDEADNFMRQDFSSLRKILKEGREYGVGAILSTQEITHFKT
                     GENNYASYILTWVIHRVSEIKNADIKAVFNVDDKGEQESLMGQIRQLEKHFSLYVDGD
                     KRVSKMRDRAFWELVF"
BASE COUNT         2632 a         2025 c         2188 g         2555 t
ORIGIN      
        1 cgaccactgc ttccgtaccc ataatcccag gtactgatac cggttgtttt ctggtgaatt
       61 ctggcgtagc caatccctgc agatacccat ggtgtgaatg cactgtcatt ccggaagtca
      121 tagtacgcat tcagcatcag tgtgttgact gacacctcat tcttcaggtc atcacgccag
      181 taaccgcctg accagctgtc tttatctacg ttatacttcg aatcagcttt tccacgagcg
      241 taaaactcca gttccgtacg aaccggaata ctgaactgcg ggtaaaaatc ataaccggcc
      301 gcgataccgc cactgaatac cgtatcatgg ccatcaccgc ctttatattt tgatgtttcc
      361 tctccatccc ccgacaggaa acgctggtct gcaagtgaca taacagaagc acctgcttta
      421 ccggtcacat aaaagcctgt tttgctttca tcagccagag cctgggtcga aaacatgcct
      481 gccatggcaa gcgctgaaac cgcaataacc ttattcattt caatcatctc cgctatcact
      541 ttccccgaac gataaatggg gttgctcatt ctaaaaccat gaaaaagata aggtaaaagg
      601 aatgttttcg atcgaatcga tcgataaaat cgatcgtgcc cgataaagtt ttcttatcgt
      661 acccataaaa atggtaaggg ctggtttaag aggtaattgc aacggcatgg aagtattgag
      721 accagaacaa catgagataa agcggctgaa cgtcagatga gtcacatcgg taagggggcg
      781 tatatccata aggcagaaca tctggatgag cgcgagctga tttgcagtat ggtgggctga
      841 ttttctggat gcgaataggg agagatcgat tacgctgttt gactatgcga aaatcaatcg
      901 ggggtaatat ttagcgtatg tgtttgatta agatactact aatcaagcaa tttccaaagt
      961 ggaactggtt gatagatgga ttttccccgc catctctgga tgtgttttgt gtacggatgg
     1021 aaaagataca attttaaaat tgcccgtaat aaatgtctca aagtgcctat agatatcagc
     1081 ggatagcttt tatagaatag gtataatcct acttttgaga acatgaagtt atgagcgcaa
     1141 tcacattacg taaagcgttg ggtgtacttg cgaagtcatc ttcgttttcc gtaaccacgg
     1201 taacgcatcg tcaaaaggat gagttcgatc agttaaaaga acaactcttt gttaaacaag
     1261 aaattgaaac tgagctgcaa cgttatctgg atgttgctaa gcccggtgaa atcatctttt
     1321 tgtgtggcag tagtggtgat ggaaagtccg aaattttgac ccgttgtaag tcaaaccctc
     1381 gttatcagca gcgctttagt tttcatctgg atgcaacaca cagctttgct ccccggcaat
     1441 cagctattga tgctttaaat gatttattta gcaatcatca ccaatactca tctcctttgc
     1501 ttataggcat caataccggg atgctcgcca attttgccag agaaggtgct gaatgccatc
     1561 tggcgatccg cactgcgatc gattctttct tgtcagcaga tcaggaagaa agccgcccat
     1621 accgcagtgg gcattgttct ttttttgact ttgagcatta ccctaaattc cagtttaatg
     1681 aaaaaaaaca gtattcatcg tttattaaaa cattactgga taatttaacc cgtaatgatg
     1741 atagtaattt gtttcagttt atttttcgac atgatgagac cgttaaccct gagctgaaag
     1801 aagttgctaa ctataaatta ctttgtctgc cgggtgttca ggatgttctc atcactcagc
     1861 tctttaaggc ccgcctgatt aaagatcagt ttgtgacaac ccgaactcta ctggattttc
     1921 tgcatcattt gcttatgggg ccggggtatt tatttgataa tcttttcact ggggcagaaa
     1981 acgacctgat taaaaaagtt tctgattttg atcctgctag gctgcacacc tatgaaatcg
     2041 atcagtttat tttgcgctac gaattgggcc tggttgaccc tgaactggat gattttttag
     2101 ctgcactcgc gccattacac attagatttg atcgccagtg cgttaatccg ggtgatgcgg
     2161 cttcattaat tcggctcttc tggttgctgc aggatgaatc tctggggaat aattatcatc
     2221 agaaattttc cgtattcttc aatgaatctt tatttgagca ttattcagaa atctggcatc
     2281 tgcacaaaaa ttacatcgct gactcagagc agaaaaaagc actgaaccgg ttttatacat
     2341 ccgaacttat tgctggtatt cagcgctatg caaaccgcaa agcgccggaa ttgagtatgc
     2401 aaaaagaaga gttcttttta ggtgaatatg gtggggtaaa aataaccgcg ccagtcgaat
     2461 tgaaacctga ctgggaggcc atccgcaata aaaatacggc ccaccctact ggcttcgacg
     2521 tgcatttaaa agttgggcaa aactctctgt taccagttcg tatcggactt aatttatttg
     2581 aacttttgaa caaattaaac aatggctatc gccccaataa atacgataaa aatgccattg
     2641 ttctactgga cgaaatcgtt gatttgatta ctgaacaggc caaatccagc agcgaaatca
     2701 agttttatgc tggtgggcgg cgagtttatc gcgctaaagc ggacgacgac atgatcacca
     2761 ttagcggtat ggagggttag tggaatgtac cctatcgcaa cgacgttaaa agtaggtaat
     2821 aatcaactgg atagttatct gccgatacgt aacaaaaaca atgatattaa ctggcaattt
     2881 gtcaccggat tggtgcttag ctacgccctg aagcgtaaaa ttgatgccta tactcccagt
     2941 caatttcgcg atgattgcaa ggctcatttg caagaattgc ttgatgaacc agcattctgg
     3001 actgtactgg aacggatgta tttttccagt gaagatatct ttcgtgtatc tccgcttttt
     3061 ttactatttc atgctcagtt tgatggcgaa aaaataagtg gcgcttcaat ggccgataaa
     3121 cgccttggca ctctgtttgc taatctaatg ggagatttca gcttggaata tcctatccag
     3181 gataagctga acttcattga gcagcaaatg ctaaataagc tcaatgaaaa aataaagtta
     3241 ttgggaaaag gcccattctc tgaagaacaa ccctaccttc cctatctggt gacttgtttt
     3301 cagtctgatc tggcgttttt ggcagaacac cctcaatatt tgttacaaga actgactaat
     3361 actctgcgtt tatatgcctt tagctggtgt gcccagctag cgctcaatct ggacaactgg
     3421 caggacggcg agccacaaag taagtcgctt ttctttattc tcgacactga aaaagccagt
     3481 tctgaacggg atcaaataaa acgctttggt tataagtggt tcgctcgtca aagtgaaaaa
     3541 cttttcccaa tactctccgc gctggaagtt ctacaaataa aaggagataa gaagcgtcca
     3601 ctctggcagg tctatcagga ttgcttaaat ttttctgata actcatctca attactgcaa
     3661 gatctcaata attatttgca ggagtttatc gaaaaacgcg agctttcggc atcgaagtat
     3721 acgcaagccg ttacgctgga agaagctttt aaacaattat taaccgttgc tgttgaacag
     3781 tttcaggata agaaatctga tcgtgcgaca gttaaccgga aatacatcaa cgagcttgaa
     3841 acccagatct gcaccgattt cattcaggta cgtggtcggg cgggaaaagt actggtactg
     3901 aatcaggatc gtctgctgct gctgaccaat ctcactgtgg gtaaaaatga caaactacgc
     3961 ctgcatgagt tgctgcgcgg ctttgaacag cgcggatttt atctggataa ccagtcggca
     4021 caaacgctgg tagcgtttta tgaacgtatg ggaaatgtgg agcgaatgag tgacagtgga
     4081 gacgccgtgt atgtccgtaa aacagtatga aacctatctg gcggaaacct ttattgaatg
     4141 ggttagtggc attattcaac ctggtgagcg ttaccagttt aaatctcctg accctgacaa
     4201 tgctctacaa ctctggcagg cttttgttga cctggcaggt gacaatcatc tggaaattgc
     4261 gccagagcag cgccttgcct gtctctcctg taatggtatt cagcttattc cggtgctgca
     4321 cggagccgca gcgccagcat tcacggaaaa ctacatatcc catttacgcg atgaagttgc
     4381 tggccgcagc ggggtgtttg ccaaaactgc actgctgatt attcataaca gcatgctgga
     4441 caccctgatt aacagcacaa aagacgtcgc ggcaccggga gctatctggc accccgaaac
     4501 cttcagtcat gaattggaaa agttgatcac gacggacaac aaccgttcag agttgtcgcg
     4561 ttgcttatta aaggatcaac gaacaacggt actggatgaa ggtgcgaccg tatttggctt
     4621 ctcgtcgttg tatcgcctgc tggatgacgg caatctggat ttctccgagc tatcgctgtt
     4681 taaagatgat gaactactga attttagcca gaaacagctc catacccgac tgaatgaaaa
     4741 caggaagtta tttcgtcaaa tcgaggacag cgttgaacgc tacagtgggc aactggagaa
     4801 cgtattaact gaattcagcg ccaaatttat tcaggaacat ttcaatgata aagacgactg
     4861 gcgcgagctt gatttcgctg tttatctaaa cgaaaaagaa cagaaccgcg aacaaaaact
     4921 ggtactggat gatatcacgg tcgaaaatgg cgtgatttgg caacgggcga aaagcgcaag
     4981 taaagcaggc aagcgcgata tcagcctact ggtacaggta ccacccgaac aatcgcaaac
     5041 tgagctggag ttttgctttc agggcaacga tcttcaggat aaccagataa aaattgcgca
     5101 tcatcgtcag ttaaaaaaag agcgtttctg gcgaattagt cgtgcagggg gtaaaagctc
     5161 ccgtattatg gcgtcggttc ctttcgatgg gaatccctgc ttctttagcc tcgaactgac
     5221 taatcgcaat aattcagccg aagagtacaa gttccgcctg ttactggtca gacaaggaca
     5281 attctggcta gacgatattc agcattgttt ccgtatcgag ccaggtaaac cacaaatcac
     5341 actccaacgc gaagacaacg aactacgaat cgctgagtcg ggtagccaag tctgtatact
     5401 ggatgaagaa aatggtgata ttgactgcca gcattatgca ttggtgaatt ttgaaacact
     5461 ggcaaatcaa agcgatctga tccaatttaa gcttgtttca ggtgattctt gtctggcatt
     5521 caatatcgaa gggccgggag ccgaagaagg gttaacctta ccgctacttt ttgaccagag
     5581 tcgtttcaat aaactgttca aagaagacgg gaatgcgtcc tggaaccgat tgaagggtcg
     5641 tatcattctt gataataccg aacacaaggt tgtcggtgtt cgccagcagt tactggcgct
     5701 ggaagcctca ttagttgatc aacgtctttt ggggacagga gatgacgata gcgcgtttgc
     5761 gctggatgaa ctcgttgcca tccaccccga tctctataac gcctacgacc aattattcct
     5821 ctactaccag cgctgcggta ctttacctag cctggtatcg tggtcagcag aatactgcgc
     5881 gctggtaagc catatagtga cgacctttga acaggcgctt caacaaatcg agttgagcag
     5941 agcgttaaca gcgcaggaaa agcgcctgct gcatctgggt atttgtaatg tcgacagtca
     6001 tgaacgtctc tccccgttgc atcccctagt gttggcctac catttacaac tggtacaaac
     6061 tatctgcgca gagcaggaac aatacgactc agcgtcattt gccacactac cgaccattac
     6121 gcttgatcgt ctggtggtgt ccggcttgat gccattcgtt taccacagtg aacacgaata
     6181 cgcacagttg cagccggtgg aagaaaatcg cttctggatc gatgtcgttc cccagcgcca
     6241 ggtgagccac gattacgtaa aacgtttggt aaaagacaag ctcaatgaat ttaccgaagc
     6301 ctacgcccgg ctatttcaga gtcccggaaa caatgcattg attattaatg ctattaatca
     6361 gggaacagcg aaagaactgt ttctggggtt agtggaatat tttaagcagg aaaaagagca
     6421 tgctatttcg gtgcatgtaa attgctacga tgaacgcctg ctgccaaata tgtttgatcg
     6481 ctttgccgag agtggcagct atgaacagct taaaaacgac cttgacctga accgcggtgc
     6541 gtggcgagct gaggccgata tgctgattga cctgttacgc agtcggttaa cgttcagcaa
     6601 atttgtcctg ccgtcagaaa gcgacaaatt ggcctatgca catttggcgt ttttcaccaa
     6661 tacagctccg gtggattgcc gccagatccg cattgaggat gctgccagcg gtgtactgtg
     6721 tcatgggctg atttccggag aaggagctga aacacagggg gatgcttact ttaccgcgtt
     6781 tggcctgcgg aatgttgata ctgaatctta ctgcgctcta cgacttgccc ggttagtggg
     6841 ctgcctgtgg cagccagcgc gtcagagcaa cagtcaatac cacggacaag ggattggatt
     6901 ggccgtaagc ggcaatttta aacagctact caattattct tacaacagtg cactctggac
     6961 caccattatt gaccctaaag ttactcttga cttctttact agccagaaag atgttgtgct
     7021 gatccactat tccgatcagt acactagttg tgcaggctac gatgcagtga cggtaacgaa
     7081 gcaggtcgat ctattcctcc gcctgctcca gaccgaaagc cagtccgggc aatctgccgt
     7141 tgatagccag catctacttg ccgaatttaa tgcttttaac ggtgagtggt tgctgaagat
     7201 gttgcgctcc agtgaaaaag agcgcaaaga gaaatatggc atcattggcg cgtataaatt
     7261 tgtacagtcc atgctgagtg agtctgatat ctgctgggta ccgctatctg tagccgaaat
     7321 gattcgcgta tctggtaacg tcgggctaaa gatgaaggaa agcgatttat caagaaatct
     7381 acaggggtac cgtaaaggcg caatctctga tgatgtgctc tttgttggtt ttaaagaaaa
     7441 ccgtctctat ttactgccgc tggaagtcaa aaccggcgct cgaccagatt acaactatgc
     7501 gggccagcag gctgctgagc ttaaacgtta tttacagcag gatattctgg agccgcatac
     7561 actggcatcg caactttatc gcgcgctctt tattcgacag gtgctgatgc aggtagagaa
     7621 acttcagctg tacggtgtgc tggacagtga taagctggct cccctgttgg atcgccgcga
     7681 atggtggttg accggtgact atcagttggg tgagttaaaa gactatgcta atggctttgt
     7741 tgttgcacat gtagacagcg gatcttgctt cgatctctcc tataaagaga cgacagagaa
     7801 tatcttacag atcgaaattc cttattcatt actatcctcg ttaatcacta cccgtgaagg
     7861 taaattgcca ttggctgaac gctaccgggt accagataaa taccgtctga agccagagag
     7921 tgatgagcac ccatctccat cagcatcggg agtgcaggta acgacaccgc cagacacacg
     7981 acctgatatt ccaaagccga caccagaggt cagcactgta cccttacaag tattatttgg
     8041 tcatgacgct acacggcaaa ccccgttatt ctgggaaccg accaacacca cgaagtttat
     8101 gaataccaac acaggcatca tcggtacgat ggggaccggg aaaacgcaat ttaccaaatc
     8161 gctggtgaca caattaatgc gtaaccaatc ctgtaacgtt gatggaaaat cgattggttt
     8221 actgatcttt gattataaat ctgactatgt ggacgaggct tttctgaagg ctactggcgg
     8281 taagaaatat caattatcgt tactacctta taaccctttg tcactgtttg gtgatatgcc
     8341 catgttaccc agacataccg caatcgcatt ttctgaaaca atggggaaag cctataacct
     8401 gggtgtgaaa cagcgtatga aactggttac gctaatcatg gagtgctatg aattagcagg
     8461 aatagtgcct catgatcgct caacctggaa ccgtgtagca ccgaccatag aagatgtgtg
     8521 gcagcgatat ctggctcagg aaaaagtaga agaagactca ttgtacgccg cgctttataa
     8581 cttagctggg tttcagatat ttgaaacaga cccagagaaa atgacgagtc tgtatgatct
     8641 ggtggatggt gtcacagtta tcgaattggc tggctactca tcagagattc aaaacctggt
     8701 ggttgcgcta accctggatc tgttttatgc acaaatgcaa aaacgcggca agccagtgat
     8761 tcagggggat tatcgtcagc tcaccaagat gattctggtg gatgaggcag ataactttat
     8821 gcgccaggac ttttccagtc tgaggaagat ccttaaggaa ggtcgtgaat atggtgttgg
     8881 agcgatcctg tcaacgcagg aaattaccca cttcaaaact ggagagaaca attacgcttc
     8941 atatattcta acctgggtta ttcatcgtgt gtccgaaatt aaaaatgcgg atatcaaggc
     9001 tgtattcaac gtggatgata agggcgaaca ggagtcgtta atggggcaga ttcggcagct
     9061 tgaaaaacat ttcagtctgt atgttgatgg ggataagcgg gttagcaaga tgcgagatag
     9121 agcgttttgg gagcttgttt tttgattatt tttgtagata gtacaaggtt agggtaaaaa
     9181 gcatgtcgag aaatgatatg atacattggg tgaacttgtg gaaagatatc ttaggattat
     9241 atccaagaat cattcagcca aaatttattt ttatatcttt ggtgacatta tttgtatcaa
     9301 cctgcggaat atggattggc ccattatttt taaaagataa tacgcagttg ggtttttcta
     9361 ttttttcatt tattattacc acattgggcg tattagccgc
//
LOCUS       DBBGLQ010000142         9306 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.142, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000142 DBBGLQ010000000
VERSION     DBBGLQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.142"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..400
                     /locus_tag="ACIYSB_004713"
     CDS             <1..400
                     /locus_tag="ACIYSB_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5431591.1"
                     /translation="ISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQK
                     LSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVP
                     LGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            734..1666
                     /gene="ydeP"
                     /locus_tag="ACIYSB_004714"
                     /pseudo
     CDS             734..1666
                     /gene="ydeP"
                     /locus_tag="ACIYSB_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
     gene            1768..3453
                     /gene="yddA"
                     /locus_tag="ACIYSB_004715"
     CDS             1768..3453
                     /gene="yddA"
                     /locus_tag="ACIYSB_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGC5431592.1"
                     /translation="MITIPITLRMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWESAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            3491..5863
                     /gene="yddB"
                     /locus_tag="ACIYSB_004716"
     CDS             3491..5863
                     /gene="yddB"
                     /locus_tag="ACIYSB_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGC5431593.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGRLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLQQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            5920..8703
                     /gene="pqqL"
                     /locus_tag="ACIYSB_004717"
     CDS             5920..8703
                     /gene="pqqL"
                     /locus_tag="ACIYSB_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGC5431594.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYVFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLPTRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            9065..>9306
                     /locus_tag="ACIYSB_004718"
     CDS             9065..>9306
                     /locus_tag="ACIYSB_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGC5431595.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         2683 a         2057 c         2107 g         2459 t
ORIGIN      
        1 tatttcaaat aacaatgttg ttatgccaac aggcggctgc accgttgata gtcgtaatgt
       61 caccgtggat ctcccggact ttcctggaag cgcagaaata ccccttggcg tatattgctc
      121 aagcgaacaa aagctctcat tttatctttc gggtgccacc actgacagta gccgacaagt
      181 gtttgctaac acagcacctg atgcaacaaa agcatcggga gtgggtgtca ctctaatgcg
      241 taatggaaaa atactggcta cgggagaaaa tgtctccctg gggacagtaa ataaaagcaa
      301 agtacctctg ggtttaagcg caacctatgg acaaacagga aacaaggttt cagccggaac
      361 cgtccagtca gtcattggtg tcacttttat ctatgaataa ttgtgatttt gaccttagtc
      421 tatgcgctcc attcacgaaa attgcgcgga taattatttt gtgaaggcta ttagcctaca
      481 cctgtaagaa aatccgcgca caattgaagc cagagtctta ttttttatat tgaaaaatat
      541 gttaatttaa acactatgag agatgatgct taacttatcg tatttaatct attgtttaac
      601 gataatagat taaaaataga aagagagtag tcccctactc ttatatatcc atgttggcga
      661 taatcccttt gtatgtactg tgcatcatcg ctattacaaa tcctaataat tcatttccac
      721 acaggataag tagatgaaga aaaaaattga atcctaccag ggtgctgcag gtggttgggg
      781 tgctgttaaa tccgtagcga atgcagtacg taagcagatg gatatacgcc aggatgttat
      841 tgccatgttt gacatgaata agccagaggg ctttgactgt ccgggttgtg catggccaga
      901 tcctaagcac agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga
      961 agtcacggat aagcaggtaa acgcctcttt ctttgctgag aatacggttc aatcattact
     1021 tacctgggga gaccacgagc ttgaggctgc ggggcgactc actcagcctt tgaaatatga
     1081 tgccgtcagc gactgttaca agccattaag ctggcaacaa gctttcgacg aaattggcgc
     1141 acgccttcaa agctatagtg atcccaatca ggttgaattc tatacttcgg gccgcacttc
     1201 caatgaagct gcctttcttt atcagctttt tgcccgtgaa tacgggagca ataactttcc
     1261 cgactgctcc aacatgtgcc atgaaccgac aagcgtgggt ttggcagcga gtatcggtgt
     1321 aggtaaaggg accgtgttgc tggaagactt tgagaagtgc gatttagtca tttgcattgg
     1381 gcataaccct ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg
     1441 gggagcgaaa atgatcgcca tcaatcctct acaggaacgt ggcctggagc gatttaccgc
     1501 accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact cagttggcca gtgcctacta
     1561 taacgtgcgc attggtggcg atatggcgtt gctcaagggg atgatgcgcc tgttaattga
     1621 gcgcgatgat gctgcaagcg ccgcaggtcg gccttcattg cttgatgtat tcctttatat
     1681 ttcatattgt taaatgatac ttaaataaat ttcatcagta aatcattgcc atagataatc
     1741 attggatggc acaatcaatt ttcgctaatg ataaccattc ccattacgct tcgtatgttg
     1801 atagcaaagt atctctgtct gttgaagccc ttttggctgc gtaagaataa taaaacttca
     1861 gtgttgttaa tcattattat cctcgccatg atccttggcg tggttaaaat tcaggtctgg
     1921 ttaaacgact ggaataatga ttttttcaat gcgctaagcc agaaagaaac cgataagctt
     1981 tggcagcttg tcctttggtt ccctgccctg ctggggatct ttgtattaat atccgtaaac
     2041 aaaacatggt taattaaact actgactatt cgctggcgcg aatggttaac agattactac
     2101 cttaatcggt ggtttgcaga taaaaactac tacttcacgc agatctacgg cgagcataaa
     2161 aatacagata accctgacca acgtatcgct gaagacattc tcttactgat cagcaaaacg
     2221 ctcagtcttt catttggctt catccagtca cttagcatgc tgatcacctt taccgttatt
     2281 ctttgggaaa gcgcgggtac gctctcattc actgttggtg gaacagaatg gaatatccag
     2341 gggtatatgg tctataccgt cgtgctcatt gtgatcggtg gaactttatt tacccataag
     2401 gtgggtaaac gtattcgtcc gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga
     2461 acaaatcttg tgcagcataa taagcaggca gaactgattg cgctaagcaa tgctgagtct
     2521 ctacaacgcc aggagttgag cgacaatttt cataccatta aagagaactg gcatcgttta
     2581 atgaatcgtc agcggtggct tgattactgg cagaatattt attcgcgctc gcttagcgtt
     2641 cttccctact ttctgttatt gccgcagttt attagcgggc agatcaatct gggcggactg
     2701 atgaaatcgc gccaggcatt tatgctggta tcgaacaatt taagctggtt tatttataaa
     2761 tatgacgaac ttgctgaact ggctgcggtt atcgatcgct tgtatgagtt ccatcaactc
     2821 actgaacagc gccctacgaa taagcctaaa aattgccaac atgcggtaca agtggctgat
     2881 gcgagtattc gtacgcctga taataagatc atattagaga acctgaactt tcatgtttcg
     2941 ccaggcaaat ggctattact gaaaggctac tctggtgcgg gaaaaaccac actgcttaaa
     3001 acattatccc actgctggcc gtggtttaaa ggtgatattt cttctcctgc tgacagttgg
     3061 tatgtgtcac aaacaccgtt aatcaaaacc ggcttactga aagagattat ttgtaaagca
     3121 cttcccctgc ccgtagacga taaatcgttg agcgaagtac tgcatcaggt tggtcttggg
     3181 aaattggctg cgcgtattca tgaccacgat cgctggggag atattctttc cagcggcgaa
     3241 aaacaacgta tcgccctggc acgattaatt ttacgacgtc cgaaatggat atttcttgac
     3301 gaaactacct ctcatcttga ggaacaagag gctatccgct tactgcgttt agtgcgtgaa
     3361 aaactaccca caagcggcgt cattatggtt acacatcaac ccggtgtctg gaacctggcc
     3421 gatgatattt gtgacattag cgcggtttta taaaaacaat aaaagccctg ctgtacggag
     3481 atataaataa atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg ctgatgtggc
     3541 gcaggccgtc gatgacaaaa acatgtacat gcattttttt gaagagatga cggtctatgc
     3601 tcctgtccct gtacccgtaa acggcaacac gcattacacc agtgaaagca tcgagcgttt
     3661 accgaccggg aatggcaata tcagcgatct gctgagaacc aaccctgcgg tacgcatgga
     3721 ttcaacgcaa agtacctcgt tgaaccaggg agatattcgc cctgagaaaa tctctattca
     3781 cggtgcgtcg ccctaccaga atgcctattt gattgacggt attagtgcaa ctaataacct
     3841 gaacccagcg aatgagtccg atgccagtag tgcaaccaat attagcggga tgtcacaggg
     3901 gtattatctt gatgtcagct tactggacaa tgtgacgctt tatgacagtt ttgtgccggt
     3961 tgaatttggt cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga
     4021 tgatagcaag gtgaaattgg gttatcgcac tacgcgttcg gactggttaa catcgcatat
     4081 cgatgagaat aacaagagcg catttaatca aggctcttca ggaagtactt attactcccc
     4141 agattttaaa aagaactttt ataccttgtc gtttaatcag gaactcgctg ataacttcgg
     4201 cgttaccgcc ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa
     4261 tgacggcatt gtcgccggtc gggcacagta taaaaacgtt atcgatactg cattaagcaa
     4321 atttacctgg tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc
     4381 cagccgtgat tataatacca gcaccttccc gcagtctgat cgcgaaatgg gtaataaatc
     4441 ctatggtctg gcatgggata tggatacgca gctcgcatgg gccaaactgc gtaccaccgt
     4501 tggttgggat catattagtg attatacccg tcacgatcat gacatctggt acaccgaact
     4561 ttcatgtaca tatggtgata ttacagggcg ttgcacccgt ggcggattag gacacatttc
     4621 ccaggctgta gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgtggg
     4681 taatgtttcg catcaaccct acttcggcgc ggaatacatc tattccgatg cgtggactga
     4741 acgccataac cagtctgaat cctatgtgat taatgctgcc ggaaagaaaa ctaaccatac
     4801 catttaccat aaaggtaaag gccgcctggg aattgacaac tacacgctgt atatggcgga
     4861 tcgcattagc tggcgtaatg tgtcattaat gcccggcgtg cggtatgact atgacaacta
     4921 tctgtcaaac cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctaatca
     4981 aacctcaatg attacagcag gttataaccg ttactatggc gggaatattc ttgatatggg
     5041 attacgtgat atccgcaata gctggacgga atcggtatca ggtaataaaa ctctgacgcg
     5101 ttatcaggat ttgaaaacgc cttataacga tgaactggca atgggattgc agcagaaaat
     5161 cggtaagaac gttattgcgc gtgcaaacta tgtttaccgt gaagcgcatg atcaaatcag
     5221 caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg
     5281 caaaaccaaa acgcattcgt tcagcctcag ttttgaactg gccgaacccc tgcatatccg
     5341 ccaggtagat attaacccac aaattgtctt tagctatatc aagagcaagg gcaacttgtc
     5401 gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa
     5461 tctggtctct tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt
     5521 aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca
     5581 agaagcgcgt aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata
     5641 ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg
     5701 cttgtcctgg acgccacaat ttctgcaaca acaaaacctg acgatcagtg ccgatattct
     5761 caatgtactg gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc
     5821 cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt
     5881 aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta
     5941 ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc cttaccgcag
     6001 gatgaaaagt taattaccgg gcaactggac aatggcttgc gatatatgat ttatccgcat
     6061 gctcatccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa
     6121 gaagacaatg agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcacaaaa
     6181 acatggccgg gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtcgc
     6241 gatgttaatg cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg
     6301 cagaaacaaa atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc
     6361 tttgaaaaac tcgaagtaga cgctgaacgt ggcgtaatta ctgaggaatg gcgtgcccat
     6421 caggatgcga aatggcgcac ctctcaggcg cgccgccctt tcctgctggc aaatacccgt
     6481 aatttagacc gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa
     6541 ttgcgccaat tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc
     6601 gatatcgaca gtaaagaagc gctggcgctg ataaaggata atttaagtaa gcttccggct
     6661 aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat
     6721 atcattaatg ataaagaaaa ccgggtgaac ggtatcgcac tctattatcg cctgccaatg
     6781 gtacaagtga acgatgagca aagctttatc gaacaagctg aatggagcat gttagttcag
     6841 ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc
     6901 ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat
     6961 gcacgagacg ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt
     7021 gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg
     7081 ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg
     7141 gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg
     7201 aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga
     7261 aagaaccagg acgcattttg ggagcaaatg gtaaacaatg aggttgccgc caaaaaagca
     7321 ttgtctcctg cggctatcct ggcgctggaa aaggagtacg ccaacaaaaa gctggcggct
     7381 tacgtcttcc caggcagaaa tttatcgtta acagtagacg cagacccaca ggcggaaatt
     7441 agcagcaaag aaacgctggc ggagaatctg acatcattaa cactttccaa tggtgccagg
     7501 gttattctgg caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat
     7561 aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca
     7621 gttagcggaa gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa
     7681 aattcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg
     7741 cggactaata accctgaacc tggttttcag ttgattaacc agcgaatcac ccacagcacg
     7801 attaacgata atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc
     7861 gaccagcgtc cagcggagaa attcgcccag cagatgtatg agacgcgcta tgctgatgac
     7921 cgcacgaaat tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct
     7981 gccgatcgcc aattgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc
     8041 gcagaagaca aactcgtggc gttaattacg cgttacttag gatcaatcaa acactctgat
     8101 tcgccattag ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta
     8161 aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca
     8221 cctgttaatc tgccgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat
     8281 ctacgtgtta atattcgtga acaggcatct ggagcataca gcgtttcttc tcgcctctcg
     8341 gttgatcctc aggccaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga
     8401 catgatgaac tgttaacgtt agcgaatgaa gtgatggtta agcgtctggc taaagggatc
     8461 agtgagcaag aactgaatga ataccagcaa aacgttcagc gcagcctcga tatccaacag
     8521 cgtagcgttc aacaattagc gaacactatt gtaaatagtc ttattcaata tgacgatcct
     8581 gcagcatgga ctgagcagga gcaattgttg aaacaaatga cggtagagaa tgttaacact
     8641 gccgttaaac aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa
     8701 taataacagc cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat
     8761 gtaataattt tataaatgcg ttcaaaataa taatcaagta ctaatagtga tattttaagg
     8821 tctgattttt acgtgataat tcaggagaca cagaatgcgc ataaaaataa cagcataaaa
     8881 caccttacca ccacccaaga atttcatatt gtattgtttt tcaatgaaaa aatattattc
     8941 gcgtaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
     9001 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
     9061 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
     9121 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
     9181 cgatgtcgca ttccagatta tcaatgacga attatatctt gatggcaacg ctcgtcagaa
     9241 cctggccact ttctgccaga cctgggacga cgaaaatgtc cacaaattga tggatttatc
     9301 cattaa
//
LOCUS       DBBGLQ010000143         9249 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.143, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000143 DBBGLQ010000000
VERSION     DBBGLQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.143"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..1967)
                     /gene="yjcS"
                     /locus_tag="ACIYSB_004719"
     CDS             complement(<1..1967)
                     /gene="yjcS"
                     /locus_tag="ACIYSB_004719"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGC5431596.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWV"
     gene            complement(2240..3169)
                     /gene="alsK"
                     /locus_tag="ACIYSB_004720"
     CDS             complement(2240..3169)
                     /gene="alsK"
                     /locus_tag="ACIYSB_004720"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HGC5431597.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLRDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLVAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQFCAKAP"
     gene            complement(3153..3848)
                     /gene="alsE"
                     /locus_tag="ACIYSB_004721"
     CDS             complement(3153..3848)
                     /gene="alsE"
                     /locus_tag="ACIYSB_004721"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HGC5431598.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            complement(3859..4839)
                     /gene="alsC"
                     /locus_tag="ACIYSB_004722"
     CDS             complement(3859..4839)
                     /gene="alsC"
                     /locus_tag="ACIYSB_004722"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HGC5431599.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(4818..6350)
                     /gene="alsA"
                     /locus_tag="ACIYSB_004723"
     CDS             complement(4818..6350)
                     /gene="alsA"
                     /locus_tag="ACIYSB_004723"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HGC5431600.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGIVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(6477..7412)
                     /gene="alsB"
                     /locus_tag="ACIYSB_004724"
     CDS             complement(6477..7412)
                     /gene="alsB"
                     /locus_tag="ACIYSB_004724"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HGC5431601.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(7471..8361)
                     /gene="alsR"
                     /locus_tag="ACIYSB_004725"
     CDS             complement(7471..8361)
                     /gene="alsR"
                     /locus_tag="ACIYSB_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HGC5431602.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            8720..9169
                     /gene="rpiB"
                     /locus_tag="ACIYSB_004726"
                     /gene_synonym="alsI"
     CDS             8720..9169
                     /gene="rpiB"
                     /locus_tag="ACIYSB_004726"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HGC5431603.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         2244 a         2294 c         2270 g         2441 t
ORIGIN      
        1 ttcacccaca aatcgaaatt atccagacag gcgataattt cttccagttt cgcgccattg
       61 ccaataattt tggctttctt cgcttttacc agatccgcca ttttggcttg tccggtcagc
      121 acggcgtgca gatcttcacg gctgatgtag aaagaggcgt cggcttgcgg ttgcagcgtt
      181 ttccggtagt taagcacgct atcgttcagc gtcaggttga ggttatcgcc gttgctcata
      241 ttgaagttca ggctgatatt tttacccgca gctttcgcgc tatcgaggcg aacggccata
      301 aagtcgaaca gcatttcgac cgacatcccg cgaatggtgt ccggggaacc ggtggtgccg
      361 tggctgaact tatgcacccc ttcgcgcagc tctttcgcgc cggtcaggta gaaaccgcgc
      421 catgtggcgg actcggcctg atagcccagc tgttcaaagt tattcgcttg cagattcttc
      481 gcgacctggt cacccgggtt ggcggcaatc acctgtttca gcagttctgc cgaccagcgg
      541 taatcacctt gcttgttcgc ttcttgcgcc aggttgatga cacgggcaga accgcccagc
      601 gcctgcacgt aacgtttacc catctccacc tgaccatacg gatgcaggtt agccgggtta
      661 ccgtcgtaat agccaagata gaagttatac accgcgcggg cgttgtggct gacagaaccg
      721 taatagccgc ggctggccca gttattggca agtgcaggcg gcagcttaat catgtcgccg
      781 atttcattca tggtgtagcc ctggttcgcc aggtgcaggg tctggtcgtg aatgtacttg
      841 atggtatcgc ggtatttacc aatataatca ttgatatgct tattgcccca gaccggccag
      901 gtgtgcggca taaacagcac ttccgcgtcg ttaccccaca tatccagcgt ttcgttcaga
      961 tactcggtcc acttgctggt gtcgcgggtt ttcgcgccgc gcagagtgta gaagttgtgc
     1021 agggtatgcg tggcgttctc ggcggtacac agggctttca gggccggaat atagaagtgc
     1081 atttcggctg gcgcttcgct acctggggtc atcaggaagt caaactccag gccgtcgata
     1141 atcatcttct cgccagttct gacgatagtt ttcgtcggtg caataatgct cgggtcgccc
     1201 gttgccagcg tcacgccaag gccattaccc acattgcctt gcgcgttgtg cggcagtaac
     1261 agaccgtaag agtagagcgc acggcggctc atgatgttac ccgccagcac gttttcgctg
     1321 atggcttcgt ccataaagcc tgcaggggca atcacctgaa ctttgccgga tttaacatcg
     1381 gcttcagaga taatgccttt cacgccacca tagtggtcgg tgtggctgtg agtgtagata
     1441 acggcaacaa tcggtttttg cggacgatgc tggaagtaaa ggtcaagtgc ggcttttgcg
     1501 gcaggcggcg tcaccagcgg gtcgataaca ataatgcctt tctcgccctc aacgaacgta
     1561 atgttagaga tatcctggcc gcgcacctga tacattttat cggtgacttt gaacaggcca
     1621 gaaataccgt tgatttgcga ctgacgccac aggctggggt ttacggtttc cggcgctgcg
     1681 gcattaatat caaatttgta atcgtccgcg cggtagtaaa ctttaccgtt cgcatcacgc
     1741 agaatacctt catccagcag cggggcgata aatccacgct gggcatcgtc aaaatcctga
     1801 cgatcgctaa acggtaatga tttggcgtaa ttctgattaa cctgttgggt atattgagtg
     1861 gcggctttcg cttcctcttt cgcgttagcg gtatttacca tcatgccgct ggcggcagtt
     1921 aacgcaataa caatcctgct caaacggaat aaccgagagt tattcatata aatctccatt
     1981 tatcatttat gtcgtaaata tgtaatccat gcaaaatacg tcatttaaag ctggcgttat
     2041 cataattggg aaattgcgac tttaataagt ggaagtgtga gcggaacgcg ccattttatt
     2101 aggcatttgt gctgaagagg gaaaattaca gtaaaactat cattgcagaa aataatatga
     2161 gtcgttttat gtgtttatta tatatattgt acctgtcgtt ggcacagcgg gacattgcgc
     2221 tactgtgcca acgttttact catggggctt tagcacagaa ctgtggcaaa aaacgttgat
     2281 gcgccaatat tgctgcaccc tgagcgccat taaagtcaga agatgaggcg gcaataaagc
     2341 gcacgacctg atgcggcagt ggacggcgca ggtacttttg ggtcatggca acgagagtct
     2401 cgcgtgggaa ggcgggcata tccatcacgc caccgcccag gatcaccgca tcgggatcga
     2461 acagattaat gctggtggca atggcccgtg ccgcgttttc aagcagactc tggacgaaag
     2521 gggcgttttc cgcatggacg aaaagatcgc gcaatgggta atttcggggc tgttgttcgt
     2581 accagcgtct tagcgccatt ccagagcaat tggtttccag gcacccagga ttgccacacg
     2641 cgcagtgttg ggtcatatct cccaggggga tatgacccag ttcgcctgcc acaccgtgtg
     2701 cacccgtcca cggcgcaccg ttcatccaca ctgcgaaccc catccccgta ccgagatagg
     2761 ccgccagaac cagttgttgc gtaaggcggt tttctactac gtcccaggag agttgcaggt
     2821 taacgtcgcg ggaaaactca accggacaat tcagcgtatt ttcgagctta tcggcgagat
     2881 catataaatc cgccgctgtt aacggcaggt taggcgtaga aataatggtg cgtttatctt
     2941 tactgaccag cgccggaaat cccatcacca gaccatgaca gcgagcgtta aagcgcctga
     3001 gttgctcgtc aatcatttcg ccgatacccg acaccaggcc gggagcaatg acttctgcgg
     3061 tccgcttttt ttcgcagtgt agcgtttcac cttctgctgt ccgcagacaa aagcggatat
     3121 gcgttgcccc catatccacg cccgctacga cgttatgctg tttttgcatg aggctgtacc
     3181 tcgctttttg cagccagaat ctgcgcggtc ataattctcc atgcttcgtc gatattttcc
     3241 gcatgattaa acaggccgga agtgccgacg ataaagacat ccgcccctgc cgccatcagt
     3301 ttttcgtaag ttgcctggtt gcaggaaccg tccacctcaa tttcgtactc cagaccttct
     3361 cgttcacgcc atgccttcag ttcggcaagt ttatccagca tttcaggaat gaacggttgt
     3421 ccggcaaagc cgggatcgac agtcatgacc gtaattttat cggccttatg gatatagtat
     3481 ttcatggcct caactggcgt ctccgggtta aggatcagcc ccactttcat gtcatgacgg
     3541 cggatttcat caatcaggcg gaacgcctgg ccgttgatgg tttccggatg cagagtgatg
     3601 aaatctgctc ccgcacgcgc cagttgagca atgtaatcct gcggccgcgt caccatcaga
     3661 tgacagtcga gcggtttagt tgccagtttt ttaacctgac ttacgaagaa cggtgagagt
     3721 gtcagattgg ggacaaagtg accgtccatg atatcgatgt ggaagtaatc ggcatggctg
     3781 tcgataaatt cgatctgttc tttaaatttc agcagatcca tacacattaa cgagggggag
     3841 attttcatgt tcaattcctt acttactgat aagacggtca agggcgacag ccgcgataat
     3901 taatccgccc atcaccacca gttggtaata ggtttgtacc tgcaaaatat tcagaccgtt
     3961 gttgatggtg ccgatgatca acccgccaat caccacagag aaaatgcgcc ccttgccgcc
     4021 gaagaaactg gtgccgccaa tgatggcgct ggcaatggca taggtttcaa aacccatacc
     4081 ggcaagcggt tctgcggcac cgagtcgtgc agttgagacg acgcctgcca gacctgcaca
     4141 aacaccggag atgataaaca ccaccaggat gtggaatttc acgtcaatcc cggaatagaa
     4201 cgccgaattt ttgttgccgc ccagtgcgta gatgttgcgc ccgagccgca tacgcgttgt
     4261 cagaaaccaa aggatgagcg cgacaattag tgagaagata acggggacag gtatcccaat
     4321 tacgctggcg gcaaagaagt tcacgaagtc aaatgagaag ccgtataccg agttggcatc
     4381 ggagatcacc agcgtgatcc cacggaaaat cgcgttggtg ccaagggtga tgatgaacgg
     4441 gtgtagcccc gtccagttga ccaggcagcc gttgatcgcc cccagtgcgc cgccaaccag
     4501 tacaccgcca atcatcgctg cgagaaacgg gtcaacacct gccaacatca gtttggcggt
     4561 caccataccg gaaagcgcca gaatcgcgcc aaccgagagg tcgataccag cgaccaggat
     4621 agcgaaaaac tcgcccatgc cgatcaatac cgtcacggag ctttgaacaa aaatctgggt
     4681 aatattattg gtggtcagaa aatattctgg tgacagcgaa ccaaagatgg cgacgatgat
     4741 cgccaggata aaaaaggtgc cgtatttatc ccagaacagc gcaaagttga acggtttctt
     4801 ctcgctcgct tcgcttttta ctcttgtggt aaagcccatg ccataatctc ctcttcgctc
     4861 atgtcatcgc gattcgtcag gatttgcgtc agtcgtcctt cgcagaacac ggcgatgcgg
     4921 tcgcagacgg tgataatttc aggtagttca gatgacacca tcaggatgac ttttccgtcg
     4981 tccgccagtt ggcgcatcac tttgtaaatt tcggctttcg cgccaacgtc gatgccgcgg
     5041 gtaggttcat cgaaaataat cacttccggg caacagcaca gccatttgga gatcaggact
     5101 ttctgctgat ttcccccgga gagttcggtg atattctggt ttaccgaatg acatttcagc
     5161 gccagcagtt cgcgttgatt ttcagcggta cgttgctcgt caacttcatg aaacaagccc
     5221 atcgcgcctt tatagccgcc gtctttcaga ctgcggctga tcgccatgtt ctgagcgatg
     5281 gaaaagttgg ggaaaaaacc gttatcccgg cggctttcag tgatgtaagc catccctttt
     5341 ttcacggcat ccaggggtga acgtggagag atatctttgc cattaagacg gatttctccg
     5401 ccagcgcgtt tatccacgcc aaacagacaa ttcatcagtt cagtacgtcc ggaaccgacc
     5461 agtccggcaa agcctaatat ttctccccgg cagacgctaa atgagatatc ccggaccttt
     5521 tttctgtcac gactggtgac gttccgcacc tcaaaaaccg tttcgtgcgc aaggttgctg
     5581 acattctcct tcatcgcgtt aaaacggttt tgcagttcgc ggcctaccat cagacggacg
     5641 atatcgtcat ttgacacatc gcttactatg ccgctgcaaa cgctgctgcc gtctttcatc
     5701 accgtatagc ggtcgcaaat acggcgaatt tccgccaact tatgcgagat atagacgatg
     5761 gccgtaccct ctttacgcaa ctgattcatg atcagaaaca gatagtccac ctctttattg
     5821 gtgagtgagg aggtgggttc atccatgatg atgactttgg catcgagcat cagcgttttg
     5881 gcaatttcta gcatctgctt gtggctgata gataaattcg ccactttctc atctagatca
     5941 actttcaagc ccacgcgtaa taacatcatg gcggcgcgga cacgcatttc tcgccagtcg
     6001 ataatattga cgccacagat ttttttcgtc agatgacgac caatatataa attttccagt
     6061 acggttaatt catcaataac gctgagttcc tgataaataa tcccgatacc gagttgtgcc
     6121 gctaatttat gatccagctt gttatagcta atgttattaa tggtaatggt gcctttggtc
     6181 ggctcatgta ttccggataa aactttcatt agcgtggatt tacccgcgcc attttctcct
     6241 agtaatgcat gtatttcacc aggataaacc gttaaattaa ccgactttaa tgcgtgaacc
     6301 ggaccaaagg acttgccgat ccccgccatc gatatatatg gcgtggccat aaataacctc
     6361 tctgcacgaa atatcgtatt attgcctgat ggctacattt atcaggtcta cgttcaggtt
     6421 attttgttgg ccggataaga cacttcatta gcatcatcat ccggcatcat tcagttttat
     6481 tgagtgacca ggattgaatc gaccagttta aattccggtg ctttatccag cgggataacc
     6541 ttgccggatt tctcagcgtc aaccatcagc ttcagacccg ttgcgccgat atccgccggg
     6601 ttctgggcaa ccgtcgcggt catttgtccg gcttccacca ttttgcgggc ttccggaatg
     6661 ccatctgtac cgacgaccag cacttttccc gtttttccgg cgtttgcgac tgcctgagca
     6721 acacccattg ccatcgtgtc attcgcgcaa tagatcgctt taatattcgg attacgttgc
     6781 aacacgttag tggcgacatc cagtgcttta atgcggtccc agtcggcagg ctggctggcg
     6841 acaagcttga tctggcttgc ttttttgaag gcttcggtgg caccattacg acgcgcttca
     6901 ccggaggcgt taccggcttt accctcaatg attgcgactt caccaccttc agcgcccaat
     6961 ttgtcaataa tgaacgacgc gcctttcgcc ccgacagcaa cgttatcggt ggtgacaaaa
     7021 gcttccacat tgccgccagc ttttttcaga ttatccatgt cgattttttc atcgagatta
     7081 accagataaa tgcctttttt ccatgcgcgg gcgacaggca tgaccagatt cactgaggat
     7141 aatggagcga aggcgatacc tttgtaattt ttattactga gatcttcaaa taactgcaat
     7201 tgagattgaa aatcgccttc tgaaggagag gcaaaaatat caacgctgac gcccagtgtt
     7261 tttgcttcat cttcaatgcc ttttttcata tctacccaaa atgggttgga gagggttttc
     7321 aatacgacag catattcggc ggcagcaaaa gcgctggttg acaacattaa gcccacgagt
     7381 gtgccgctga aatatttcag atatttattc ataataatgt tctcacggta aggggacagg
     7441 taactccgcg atggcggaat tacctttttc ttatttcagc gcgcctggtg agaagaaatc
     7501 aacaattgcg ccggtttttt gcatattaat attagcttgt tcaatgttga gctgggcgac
     7561 agagacaaaa aaagcgtcca gcaaagttag ttgtaatatt cttgccgagg cattacgacc
     7621 taataacggc gtttccgggg ctggtgagca aataatataa tcggccagtt tcgctatcgg
     7681 tgaatggtag ctatgggtta tacaaataat ctttgccccg ttcttttttg ccagttctac
     7741 ggccgctttt acatcactgg ttcgcccgga atgggtcact accagcacaa catctccttc
     7801 ctgtaacaac gaagcggaca tcatcatgat gtgagcatca ggataggcct gacagcgtac
     7861 gccaatgcgc aagaacttgt gctgtacatc agcacagata gcatttgatc ctccggcacc
     7921 gtacaaatcc cgctgtctgg cctgatagaa aaagcgggcg gcacggtgga tctcatcaac
     7981 gttgacgatc gactgacctt ccataatggt gcgtaaagtg atgttaaata ccttattcac
     8041 cacatcctgc ggcgcttcat caaaagccaa ctcggaaggc aatacctgtt ctgactgaga
     8101 aaaataatct tccagcgcac tgcgtaagtt acgaaagccg ctaaacccca gcagctttga
     8161 taccttaact atcatcgctt cagataccgc cagagcttct gcgacatctt taattgcggg
     8221 tgcacaactc aggttaccgg gtttgagtaa ccactccacg atgcggcttt cattttctgt
     8281 cattccttcc tgcttcattc gcaggtaagg cgctaaccct ataccgttcg gaagcgctga
     8341 atcaaactct gactggctca tcacgttctc ttgtccttga attgaagacg ccactatatc
     8401 accacagcaa gaacagaaaa gatgctgcct acttcacaaa aaaataaaat ttataaagtt
     8461 tattttattg tgaacaatgg cgagtggctt aaaattttat ttcatttaat tagtttaaaa
     8521 acatgatgtt atttgttttt ctggtttttt agatgataga tgttgctgga tggtgagtgt
     8581 ctttaacgct attgcgctgt cagatgtaac tctgtaaaac agatcaggaa ggcgtaattc
     8641 atcgattttg ggtgttttta attcattaac atcacaaatg ttttttgatt gtgaagtttt
     8701 gcacggacgg ggaagatgaa tgaaaaagat tgcatttggc tgtgatcatg tcggtttcat
     8761 tttaaaacat gaaatagtgg cacatttagt tgagcgtggc gttgaagtga ttgataaagg
     8821 aacctggtcg tcagagcgta ctgattatcc acattacgcc agtcaagtcg cactggctgt
     8881 tgctggcgga gaggttgatg gcgggatttt gatttgtggt actggcgtcg gtatttcgat
     8941 agcggcgaac aagtttgccg gaattcgcgc ggtcgtctgt agcgaacctt attccgcgca
     9001 actttcgcgg cagcataacg acaccaacgt gctggctttt ggttcacgag tggttggcct
     9061 cgaactggca aaaatgattg tggatgcgtg gctgggcgca cagtacgaag gcggtcgtca
     9121 tcaacaacgc gtggaggcga ttacggcaat agagcagcgg agaaattgag attcatccac
     9181 tacttgcatg gatgagtaat gattaatgtg gatagagttt ctttttgagg ttggctaatg
     9241 aaacgcttt
//
LOCUS       DBBGLQ010000144         9122 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.144, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000144 DBBGLQ010000000
VERSION     DBBGLQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.144"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(65..388)
                     /locus_tag="ACIYSB_004727"
     CDS             complement(65..388)
                     /locus_tag="ACIYSB_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGC5431604.1"
                     /translation="MKRNLMLCFVINALFNASAMALTLDDARTQGRVGETYNGYLVAL
                     KADTETQMLVKTINESRKKSYQKLAIKNNLPVAEVEKLAGQKLVAKAKPGEYIRGING
                     MWLQK"
     gene            complement(396..581)
                     /locus_tag="ACIYSB_004728"
     CDS             complement(396..581)
                     /locus_tag="ACIYSB_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGC5431605.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(578..3217)
                     /locus_tag="ACIYSB_004729"
     CDS             complement(578..3217)
                     /locus_tag="ACIYSB_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGC5431606.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKEQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTISDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVMLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3425..4414
                     /gene="ldhA"
                     /locus_tag="ACIYSB_004730"
     CDS             3425..4414
                     /gene="ldhA"
                     /locus_tag="ACIYSB_004730"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGC5431607.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4525..4947
                     /gene="hslJ"
                     /locus_tag="ACIYSB_004731"
     CDS             4525..4947
                     /gene="hslJ"
                     /locus_tag="ACIYSB_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGC5431608.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4944..5210)
                     /locus_tag="ACIYSB_004732"
     CDS             complement(4944..5210)
                     /locus_tag="ACIYSB_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGC5431609.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5484..9008
                     /gene="nifJ"
                     /locus_tag="ACIYSB_004733"
     CDS             5484..9008
                     /gene="nifJ"
                     /locus_tag="ACIYSB_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGC5431610.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGNSALAELQGVIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2267 a         2395 c         2237 g         2223 t
ORIGIN      
        1 cagttattat tgaatgtttc aggaggtaca accagacaga tttttctcga cgaataccga
       61 attattattt ttgcaaccac atcccgttta tcccgcgaat gtattctccc ggcttagctt
      121 ttgctaccag tttctgccca gccaattttt ccacttctgc aaccggcaaa ttatttttta
      181 tggcgagttt ttgatagctc tttttacgcg attcattaat cgttttgact aacatctgtg
      241 tttcggtatc cgcctttaat gcaaccagat aaccattata cgtttcgcca acacgcccct
      301 gagttcgagc gtcatccagc gtcagcgcca tcgcactggc gttaaataat gcattaatga
      361 caaaacacag cattaaattt ctcttcatac tcgcctcaga aaagatcgct acgggtctca
      421 agcagctctt cgacatcttt gtctgccttg atgatgatct catgctcaat tttaacgttc
      481 atattgatag tgatcggttc cttaggtgca gcgacttcaa tgcgaggcgt acagccaacc
      541 agcataaaag atgacgtcaa cgcagccagt aaaattttca ttgtttttcc tcacactctt
      601 ttccttgcgg acagtcattt cccggcagac gtgcgttctg ctccaaccat gcctggagat
      661 tatcgccaaa gcgtaaactg cgccacagcg taaaaatatt ctcttcatga tggtaattaa
      721 ggtttaccgt accgctttta ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg
      781 ttaataaacc gagattatct aagttaattt ttgtcgatga ccggctaatt tccatatagc
      841 gcaaccagtt aattgctgaa cccgcagtca tattgtcttt caccaccgca tctgcggtgt
      901 ctttatccag ccgcaaggtc attggcccgc tatttgccag ccaaccatct ttcactatcc
      961 atttttcgtt gtttagccat aacggcaatg caccactaaa tgccccggac atggcgaatt
     1021 gtttcggatt gacggcgcta accagttcgc tggatgagag attattcagc cgcaacagcg
     1081 ccgggtcatg ttgcggcatg cgtaattgtt tcatcagcac gttaccgcct aaaacatcga
     1141 cgctaacatc agtcaacagc aagggttctt cttcagtcca cggataacgc ccttgcaaat
     1201 cagccgtaat atttttcgct gtcaccagat taatcacttc ggcaattcgc aacataacgg
     1261 ggccgcgagt ccccagatgc caggctccat cggcaaaacg gaaaggcagg acaaaatcga
     1321 caccgttaac ctgattatct ggcatccagg cactaccgcc tttcaacacg ccgtgccctc
     1381 ccgcgcggaa tccttgttca ggcgcagcag aaaatgcaac ctgagcatac agttcaccat
     1441 cgcgtaagtt catcttccag tcgggtggca ccagcggctg gaatacagtc agtgattgtt
     1501 taggccacca ggcgttaccg cgcagacgaa taccgtccca gcgaccattt acccgaaccg
     1561 ggccaatctc accagcatgt aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat
     1621 cgacgctaaa ttttaaggtt gatggcggta acacactgcc gccagtgaac agcgtttgcc
     1681 cggcgtccag tgacagcgcg ccgctgaagg agggatgctg tgcgtcacgt acccagacga
     1741 cgggcttgtc gagaattaat cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt
     1801 cgaaaccggt ggagagatcg gtcagcgtaa tcgtgctgtc atgccactca ccctttcctg
     1861 cgacatccca ggtggcattc atcggtgtaa aactgccttt tccccagtag cgccactgcc
     1921 agcggccagc gtcagggaga aaatcattcg ccagcccatc catatgcagc acgaaatcgc
     1981 ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg
     2041 tgacttttac acccgctaaa ggccagcgga tttcatcgat gtccagcgaa tcgatgactc
     2101 ttccctttga acgaagtaac gcgcctggct caaaggtcag cgttgggtca gacagacttc
     2161 cacttagctg cgcgggtaag cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg
     2221 tcagctgtaa aggcagctgg ctgttatcca tgcttaattt tcctgggcca aaatttagca
     2281 ccgcgttgcc cttacccgct tgcccctggg tcagcacact cagtcgtccg ctgaccagag
     2341 cgttctcaag ccctgcctgc cagttgtcga ctttgacacc cagtcggcca ctcaaaggaa
     2401 aacctgcata cggccagctc cagcgaccat cgcttatggt caattgttga cgagtaattt
     2461 gccacggcaa atcgagcaac ggatcgccgt tatcccgtgc cagcacgatc aattgcccgc
     2521 tattttcctg ccagtccagc tctgcatcca ccagtgacgg ttcctgcggc aagtttaacg
     2581 tcgcagtagc atgcccactt acaggaagtc catccggcac gagcggcata gtaaattccc
     2641 ccaccagttt aaccggcggc tgattttcaa acgcgacgac atccagttcg ctgactgtaa
     2701 gttgttgctc tttcagctgg ccttgaaatt taactttttc gccctgataa cgcagttgct
     2761 ggatatcaga ggttaatgcg agagagagtt ttccctgcca ttcctgccag ggagaaaaaa
     2821 tcagtttatc gatattgatc caggtgttag gcagcatgga ctgccactgc gcgagggttt
     2881 ttggagcagc tggcgattgc tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt
     2941 ctaccgtgcc gacgttcaat aaccagcggc tcggatgtga aaggctggcg ttggtgatat
     3001 gcgcaagctg acaatctccc accagataac gaagatcggg aatgattaaa cctttacgcg
     3061 taatgcgtgg gctttcatct aatgcaatac gtgtcccgag cggtagccag atgcccgcca
     3121 gcgtgggaac ccacagcccg agcgtcatca gcagcgtcaa cggcacaaga ataatcagta
     3181 ataacagcgc gagaacggct ttatatttac ccagcatggg tagttaatat cctgatttag
     3241 cgaaaaatta agcattcaat actggtattg tggcatgttt aaccgttcag ttgaaggttg
     3301 cgcctacact aagcatagtt gttgatgaat ttttcaatat cgccatagct ttcaattata
     3361 tttgaaattt tgtaaaatat ttttagtagc ttaaatgtga ttcaacatca ctggagaaag
     3421 tcttatgaaa ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt
     3481 gaacgagtcc tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc
     3541 taaaactgcc aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccgccc
     3601 ggtgctggaa gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt
     3661 caataacgtc gaccttgacg cggcaaaaga actggggctg aaagtagtcc gtgttccagc
     3721 ctatgatcca gaggccgttg ctgaacacgc catcggtatg atgatgacgc tgaaccgccg
     3781 tattcaccgc gcgtatcagc gtacccgtga cgctaacttc tctctggaag gtctgaccgg
     3841 ctttactatg tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat
     3901 gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc
     3961 agcggcgctg gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga
     4021 cgttatctct ctgcactgcc cgctgacacc ggaaaactac catctgttga acgaagccgc
     4081 cttcgatcag atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga
     4141 ttctcaggca gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt
     4201 gtatgagaac gaacgcgatc tattctttga agataaatcc aacgacgtga tccaggatga
     4261 cgtattccgt cgcctgtctg cctgccacaa cgtgctgttt accgggcacc aggcattcct
     4321 gacagcagaa gctctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga
     4381 aaaaggcgaa acctgcccga acgaactggt ttaatcttgc cgctcccctg caatccaggg
     4441 gagctgattc agataatccc caatgacctt tcattctcta ttcttaaaat agtcctgagt
     4501 cagaaactgt aattgagaac cacaatgaag aaagtagccg cgtttgttgc gctaagcctg
     4561 ctgatggcgg gatgtgtaag taatgacaaa attgctgtta cgccagaaca gctacagcat
     4621 catcgctttg tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca
     4681 gaaatcagct ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg ctttagcggt
     4741 gaaggcaaac tgtctaatgg tgaactgaca gccaaagggc tggcaatgac ccgtatgatg
     4801 tgcgctaacc cgcagcttaa tgaactcgat aacaccatta gcgaaatgct gaaagaaggt
     4861 gcacaagtgg atctgaccgc gaaccagtta acgctggcga ccgcgaaaca gacattaact
     4921 tataagctgg cggatttaat gaattaatag ctgccacagc tcccggcggc aagtgactgt
     4981 tcgctacagc gtttgccgtt gggtaatgca cacatcccaa tcgccgtacc atccagttga
     5041 cgggcaacag aaagcgaacc gccgatcatt gcacaatttg cttctccact actggacatc
     5101 gacgctttta aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg ctcactacta
     5161 cacgccgaca acaataaagc ggcacaccct acccaaaacg ctgctcgcat ctcttcttcc
     5221 tctgatcttc aagccaaacg acaccgccat aaataatagg cagcacagag ggcggcgtcg
     5281 agagctgtcc tgcgcgttgc cccgcatttt tactttttta tggctatttt tttgccctct
     5341 gtttgatcaa aacattcatt acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa
     5401 agccatttgc taaaattgaa ggattactac tgggcgcgca gcaatttcgt gcgcccctca
     5461 tttgcgcaat gtaagggtgt catatgatta ctattgatgg taatggcgcg gttgcttcgg
     5521 tggcgtttcg caccagtgaa gttatcgcca tctaccctat tacccccagt tccacgatgg
     5581 cagaacaggc tgatgcctgg gccggaaacg gcttgaagaa cgtttgggga gacacaccac
     5641 gcgtggttga aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga
     5701 cgggtgccct ttcaacatcg tttacgtcat cgcagggttt gctgctgatg atcccgacgc
     5761 tatacaaact ggcaggcgaa ctcacaccgt ttgtcctgca tgtagcggca cgtaccgttg
     5821 ccacacatgc actttctatt tttggcgatc attccgacgt tatggcggtg cgccagacgg
     5881 gttgcgcgat gttgtgtgcg gctaacgtcc aggaagcgca agactttgct ctcatttcgc
     5941 acatcgcgac gctgaaaagc cgcgtgccat ttattcattt ctttgatggt ttccgtacgt
     6001 cccacgaaat caataaaatt gtcccgctgg ccgatgacac tattcttgat ctcatgccgc
     6061 aggccgaaat tgatgctcat cgcgcccggg cgctcaaccc ggaacatccg gtgatccgcg
     6121 gtacgtccgc caatcctgac acttatttcc agtctcgcga agccaccaac ccatggtaca
     6181 acgcagtcta tgaccatgtt gaacaggcga tgaatgattt ctctgccgcg acaggtcgtc
     6241 agtatcagcc gtttgaatat tacgggcatc cgcaagcgga acgggtgatt atcctgatgg
     6301 gctctgccat tggcacctgc gaagaagtgg ttgatgaatt gctaacccgt ggcgaaaaag
     6361 tcggcgtgct gaaagttcgc ctgtaccgcc ccttctccgc taaacattta ctgcaagctc
     6421 tgccgggatc cgtacgcagc gtggcggtac tggacagaac caaagaaccc ggtgcccagg
     6481 cagaaccgct ctatctggat gtaatgaccg cactggcaga agcctttaat aatggcgagc
     6541 gcgaaactct gccccgtgtc attggtgggc gctatggtct ttcatccaaa gaatttggcc
     6601 cagactgtgt actggcggta tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta
     6661 cggttggtat ttacgatgat gtaaccaatc tgtccctgcc gttgccggaa aacaccctgc
     6721 caaactcggc gaaacttgaa gccttgtttt atggccttgg tagtgatggc agcgtgtccg
     6781 cgaccaaaaa caatatcaag attatcggta attccacgcc gtggtacgca cagggctatt
     6841 ttgtttacga ctccaaaaaa gcgggcggcc tgacggtttc tcaccttcgc gtgagcgaac
     6901 agccgattcg ttccgcttat ctcatttccc aggctgattt tgttggctgc caccagttgc
     6961 agtttatcga taaatatcag atggctgagc gtttaaaacc tggcggcatt ttcctgctca
     7021 acacgccgta cagcgcagat gaagtgtggt cgcgcttgcc gcaggaagtt caggccgtgc
     7081 taaaccagaa aaaagcgcgc ttctatgtga ttaacgcagc gaaaatcgcc cgcgaatgtg
     7141 gcctggcggc ccgtattaat accgtcatgc agatggcttt tttccatctg acgcaaattc
     7201 tgcctggcaa tagcgccctc gcagaattgc agggtgtgat tgccaaaagt tacagtagca
     7261 aaggtcagga tctggtggag cgcaactggc aggctctggc gctggcgcgt gaatccgtag
     7321 aagaagttcc gttgcaaccg gtaaatccgc acagcgccaa tcgaccgcca gtggtttccg
     7381 atgccgcccc tgatttcgtg aaaaccgtaa ccgcggcgat gctcgccggg cttggtgatg
     7441 ccctccccgt ttcggcgctg ccgccagacg gcacctggcc gatgggcact acgcgctggg
     7501 aaaaacgcaa tatcgccgaa gagatcccca tctggaaaga ggaactctgt acccaatgta
     7561 accactgcgt tgccgcttgc ccacactcag ctattcgcgc aaaagtggtg ccacccgaag
     7621 cgatggaaaa cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg
     7681 ggcagaaata tgtcttacag gtagctccgg aagattgcac cggttgtaac ctgtgcgtcg
     7741 aagtttgccc ggcgaaagac cgtcagaatc cagagattaa agccatcaat atgatgtcgc
     7801 gcctggaaca tgtcgaagaa gagaaaatca attacgattt cttcctcaac ctgccagaaa
     7861 tcgaccgtag caaactggaa cgtattgata ttcgtacatc gcagctgatt acaccgctgt
     7921 ttgaatattc aggtgcttgc tccggttgtg gcgagacgcc gtatattaaa ttgctgactc
     7981 agctctatgg cgaccggatg ttgatcgcta acgccaccgg ctgttcttca atttatggcg
     8041 gtaacctgcc ctctacaccg tataccaccg atgccaacgg tcgtgggccg gcatgggcga
     8101 actctctatt tgaagataat gccgaatttg gccttggttt ccgcctgacg gtcgatcaac
     8161 accgtgtccg cgtgctgcgt ctgctggatc aatttgccga taaaatcccg gcggaattac
     8221 tgactgcgtt gaaatcagac gccacgccag aggttcgtcg tgaacaggtt gcagctttac
     8281 gccagcaact caacgacgtt gccgaagcac atgaactgct acgtgatgca gatgcactgg
     8341 tggaaaaatc aatctggctg attggtggtg atggctgggc ttacgatatc ggctttggcg
     8401 gtctggatca tgtattgagt ttgacggaaa acgtcaacat tctggtgctg gatacgcaat
     8461 gctattccaa caccggtggt caggcgtcga aagcgacacc gctgggtgca gtaactaagt
     8521 tcggcgagca cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg
     8581 gtcatgttta tgtggcgcag atttctctcg gcgcgcagct caaccagacg gtgaaagcga
     8641 ttcaggaagc ggaagcgtat ccggggccat cgctgatcat cgcttatagc ccatgtgaag
     8701 agcatggtta cgatctggca ctcagccacg accagatgcg ccaactcaca gctaccggct
     8761 tctggccgct atatcgcttc gatccgcgtc gtgccgatga aggcaaactg ccgctggcgc
     8821 tggattcacg cccgccgtca gaagcactgg aagaaacgtt acttcacgag caacgtttcc
     8881 gtcgtctgaa ttcgcagcag ccagaagtgg cagaacagtt atggaaagat gctgcagctg
     8941 atttgcaaaa acgctatgac ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca
     9001 ccgattaaat gctctggata aggattatcc aattctaaaa aaaaagcccg gacgactgtt
     9061 cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca acagaaaata
     9121 aa
//
LOCUS       DBBGLQ010000145         9080 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.145, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000145 DBBGLQ010000000
VERSION     DBBGLQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.145"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..958)
                     /gene="gspL"
                     /locus_tag="ACIYSB_004734"
     CDS             complement(<1..958)
                     /gene="gspL"
                     /locus_tag="ACIYSB_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HGC5431611.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHL"
     gene            complement(973..1956)
                     /gene="gspK"
                     /locus_tag="ACIYSB_004735"
     CDS             complement(973..1956)
                     /gene="gspK"
                     /locus_tag="ACIYSB_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HGC5431612.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTLSFHSQLQRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            complement(1949..2536)
                     /gene="gspJ"
                     /locus_tag="ACIYSB_004736"
     CDS             complement(1949..2536)
                     /gene="gspJ"
                     /locus_tag="ACIYSB_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HGC5431613.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            complement(2529..2906)
                     /gene="gspI"
                     /locus_tag="ACIYSB_004737"
     CDS             complement(2529..2906)
                     /gene="gspI"
                     /locus_tag="ACIYSB_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HGC5431614.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            complement(2903..3412)
                     /gene="gspH"
                     /locus_tag="ACIYSB_004738"
     CDS             complement(2903..3412)
                     /gene="gspH"
                     /locus_tag="ACIYSB_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HGC5431615.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            complement(3420..3857)
                     /gene="gspG"
                     /locus_tag="ACIYSB_004739"
     CDS             complement(3420..3857)
                     /gene="gspG"
                     /locus_tag="ACIYSB_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HGC5431616.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            complement(3867..5046)
                     /gene="gspF"
                     /locus_tag="ACIYSB_004740"
                     /pseudo
     CDS             complement(3867..5046)
                     /gene="gspF"
                     /locus_tag="ACIYSB_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
     gene            complement(5043..6524)
                     /gene="gspE"
                     /locus_tag="ACIYSB_004741"
     CDS             complement(5043..6524)
                     /gene="gspE"
                     /locus_tag="ACIYSB_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HGC5431617.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHKALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            complement(6534..8486)
                     /gene="gspD"
                     /locus_tag="ACIYSB_004742"
     CDS             complement(6534..8486)
                     /gene="gspD"
                     /locus_tag="ACIYSB_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HGC5431618.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            complement(8470..>9080)
                     /gene="gspC"
                     /locus_tag="ACIYSB_004743"
     CDS             complement(8470..>9080)
                     /gene="gspC"
                     /locus_tag="ACIYSB_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGC5431619.1"
                     /translation="NHAGVKDANPVAVNQETPKLSIALNGIVLTSNDETSFVLINEGS
                     EQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDIIKQPDSESQNV
                     LADYIIATPIRDGEQIYGLRLNSRKGLNAFTTSLLQPGDIALRINNLSLTHPDEVSQA
                     LSLLLTQQSAQFTIRRNGVPRLINVSVGELTGMNGLRHERTQ"
BASE COUNT         2123 a         2227 c         2356 g         2374 t
ORIGIN      
        1 gtaaatgata gagcaacggc acagcttcag gatattgctg agcgagttgc tgcttaaagt
       61 aaaaacgaaa attgtgggtg cgtttgatct gcgggaaata acgttgccag gtttcttgct
      121 gttgttgctg aagttgatct tcaatcttaa gcgtatgcca gagcgcgatg ctccggctgc
      181 caacaaaaga aagaatggcg agaacgagac agcttactgc caggcgggcg agccattttc
      241 ccgatttgct tacagttttc tgtttacgaa aaataccatg cagcatgtca tagcgctgga
      301 atgcaatatc ggcagaatac aaactcagtg atggtatttc aggatgctgg ataagtggat
      361 tggccgctgc aacgccgtga ggtactacac cataacaaag catattctca ggcggaaact
      421 gggcagcaag atgctgtaac cagtgttcat ccagttcgtt gaaggcatgt gcggcactgc
      481 gaatgagcca gcttgtttgc tggttaacca gagtccagct atctacttcc cagggaagat
      541 agcagccatc tggcaaaacc cgcgtcacgt tgagtccggc agtatgtaaa cgctcaagat
      601 agcgactgag tttctccgac tgaatgccta tcacctcgac actttcgttt tgtttatcga
      661 caaccgtcca gtgccagtcc tgggtgttgt cgggtaacgt ctcctctgcc agccattgta
      721 atgtttgtgc ggtgagtttg tatttggcgt tgggcagcgt gagggaacga tataaaaggc
      781 cctctggtgg gatcaaaaga tgaacatttc cggcaccggg ctgatcggcc agcgactcca
      841 gcggaagatc gtccgtgagc gtatgtaccg aagatacgct atctgcggag aacgtcatcc
      901 attcccaatg cttacgcagc gtcgaggaag aacgtatgac catcaacgat tcaggcatca
      961 cgatccttat gattagtaga gttgcagcgt acgccgatag atttgggcgc tctgctgttt
     1021 acggttgaaa aaaatatggc tttgccatcc ctgggattgt tcgtcgctga tactttccgt
     1081 actcagcgta aaatagcggc tatagggaaa gagatgcctt ttcacctgag caaccagtgg
     1141 cttcacaccg gagaaatcct gctgcgccca gtaaagaaat gcatccgtgg ttagccagcc
     1201 ttccctcggc cttttttgca ataagacccg agcgtcagca tccgtgatgt tattgaggaa
     1261 taacgctctg aagagcggaa tatcgttctc cgttagcata ttaagattga tgctaagttc
     1321 cgtggtcggg agaacgcaga catacgggat aagccgctga tagatgtttt cggttatgcc
     1381 ttttatctga cgtaattcgc cagtcagaaa aagcatctga ttggcactat gccggggcgg
     1441 cgtctggctt tggtaaaagc tatcttctgc gccgtgaaag cgtggtgagt catcgacatc
     1501 aatatagtca gcgattgatt gcacgatctc atcggtgttt ccgcgatcga taccggcgtt
     1561 aatcagcagc gcactgaata cctgggcggg gaaatcaggg gaagccagcg ggtcgtcaga
     1621 gatttttgct aacgcattga gattaaaaca gtgctgggca tcgcgtaact gccacttcac
     1681 tgtattgcca tcttccagct gcaactgttg tggctgtgcc cagtactgct gcaacgtggt
     1741 ctgtctgtcg ttatctttaa cgtcctgggt gaggctggca agcgcaagtt tctccgccag
     1801 ttcaatatca tattgccgtt gcagatgatg gtttacctgg cgggttcgct gaagctggct
     1861 gtgaaaactg agcgtcatgt ctgctgccag agcagccatg agcgccagta acatgagtac
     1921 aatgagcagc gcgacgccgc gttgttcatt attcatggtt attattcccg gcctgcgtct
     1981 gagctggcga ctcttctctc gctaactgct caggtaatgc aaaacggcgc tgtaaccccc
     2041 catattgctg agtctgcaga tgtaacgtga ctgagagagg aagttcctgg ctttccccgc
     2101 tgttactttc cagaagaaag ctctcgacat gttccagcat cgcctgggcg ggttgatcct
     2161 tcctgccatc cacagaagta cgaacagcac ggtagagcgt atgatttcgc agataccagt
     2221 gaacagtgag taaggtttgt gcttcactga gaggagccag cggatcgcga ctttgcgtgg
     2281 taaaaatagc ttcttcaccc aacaccatga ttttgtccgt actccgattc ctccgggcga
     2341 ccagttgcag gagatcgtta tcaagaatgg tgatggtgcg ctgtagctga ttgaactgct
     2401 ggatttcttt ctgtgagcgt tgatgcaact cggaagcttg cgaaaaaatc ataaacgcca
     2461 gcacgctaag cattgagaaa atcgccagcg ccgccatgac ctccagcaat gtgaaaccct
     2521 gctggcgatt aatcatcttg tgcctgcccg gatttattat cgatgctttg ataacgcgta
     2581 agcgaggttg tctgaccgct gggtaatgtc accgttatgg tgcgttccag aagcgtgccg
     2641 tctttgcttg agtggatatc gctgcgccag ttccactctt cgccatttat taactctttg
     2701 ccagaactgg acgtgttttc ctcgccgaat gaatcttgtg actgaagctg gttatccgct
     2761 atccacaatg ccagcgtctc gttacgcatt cgctcgatgg catttcgttg cccttgcata
     2821 ctgctcatta aagtcagagc aacagcggtg aaaatactca tcgccagtaa gacttcaagc
     2881 agtgtcatcc ctgattgttt gttcatgggc gggtatctct ggcgagcgtt tgatccaggg
     2941 gccatgagcc tgaacagact aacgtcagga gcggctcacc ggtcccggcg ttagccatca
     3001 acaaagagaa gggcgtgatt tgtccgtcgg ccagaatcac aacctgtggc tggttgctgt
     3061 catcaggttt aaatggctga agatgaattg agagttcttc atcccagtca ttttggctct
     3121 cgtctgcggc atcttcttgt aatggaaccc agcgccaggc tgatggtgtt ttgcccggta
     3181 ccataatgcg ccatgctgaa tcagagaaat ggataccgac aggctgacca ctgagcgtgg
     3241 cgcggtcgat ggctaattca agtgcagcgg taaaccgcgc cgctgtttcg cgcgcacgag
     3301 tgctggcgac atcctcccgg ccatatgtga agagcaccac gcttgccgtg atagcgacca
     3361 gcgccagcac cagcatcatc tccagcaggg taaacccgcg ttgctgattc atcgctgact
     3421 tactttttct tcttgctcaa accccagttg gtgatgtcgt cctcggttcc catttcacca
     3481 tcgggccctg ctgaaagcag atcgtatgca ccatgttcac caggattaac gaggacataa
     3541 tcattgcccc agggatcggc aggcagacgc ttgatataac cttccttgtt atagtttgcg
     3601 gccagcggtg gcagtgtcgg cgcttctact aaggattcaa gcccctgatt tgtggttgga
     3661 tagtgatgat tgtcgagttt gtacatatca agggcgtttt ccagcgccac aatatcgctg
     3721 acggcttttt gcttatccgc cttttcttta ttgcccatta ggttaggaac caccaggctg
     3781 gcaagtacgc cgataatgac gatcaccacc ataatttcca gtaatgtaaa accgcgttgc
     3841 ttatccgttg cgcgcatatg ctttccttaa ttaatcattg agttaagttg aagaagaggt
     3901 tggagtaccg acacgacaat aaacaggacg atcagtgcca tcgtaataat gagtgctggc
     3961 tcgaagatgg agagcgttaa ggcgatccga ttttgttgga gtgtctcctg gttatctgcg
     4021 gctctgacca ttaatgtgcc gagctgcccg cttttttcgc cagaggccac catgtagagc
     4081 atcatcggcg ggaaaattgc ggtttgttcc agcgaaagat gaatgctgtt accctggcga
     4141 acgttctctg ccgcatttgc cagacgctgg cgaatttcga ggttgttgag gctttcggtg
     4201 gacaaattca tcccatccag cagagggacg ccgctggatt gcaaaatact taaagtgcgg
     4261 agatagcgtg cgctgttaat ggcgcaaatc agcgggccga tgagcgcaac gcgcagcaac
     4321 atggcatgaa aacggtggcg gttattgccg cgttttaacc agagccagaa acctacagcg
     4381 acaataaaca ctgtcgctaa taatgtacgt gtcgctcaga cctaaaagaa tgcgtgtact
     4441 cagcggcagt tgctgcttca tatgcacgaa ctgttcggta attttgggca cgacagcagt
     4501 gaggagaata atcacgaccc caatcgccac cgtagtgagc atacaggggt agatcagtga
     4561 ctgaatgagc ttgctgcgga ttttctgccg gttttcattg taatcagcca gcttttccaa
     4621 caccggggcc agcagcccgc tcttttcgcc cgcttttacc agggtacgat agagcgaatc
     4681 gaaaagcgtg ggaaaatgct gtaatgcatc ggaaagggga tgcccttcaa ggatggcgct
     4741 gcgtacctga tttaacacgt cacccagtcg tttattactg ctttgttgac cgattacggc
     4801 aaggctctct tccaggggta atgccgctgc gcttaaggtt gccaactgcc gggtgaaaag
     4861 cgtcagttca ctatggctga tcctcgggcg acgtgttttt actcccgaac ttttttgggg
     4921 gcgaatatcc agcaggaaaa gcccttcttc acgcagccgc agtcgtgcct gacgttcatc
     4981 gttagcatca atgatccctt gcaatttttg accatcctgg gtcatggcgc gatagcgata
     5041 attcatgctt cgctctccag cgtggcactg gcgacgcgca taacctcatc ccaggaggtg
     5101 tcaccgctta tcactttttg caggccattt ttgattaagg ccttgtgttg ttgccggact
     5161 agtcgctcca gtgcttgttc atccacattt tcatgaatag cggcccgtaa ttccggcgtc
     5221 accaccatca tttcgtggat cgccatgcgc ccctgatagc cggattgatg gcaatgaggg
     5281 cagcctacgg gagtgccaat tgttgtcacc gcgagctgat gatatttaaa catctgcgct
     5341 tgttggggtg atacgggcgt gaattgtcgg cattgcggac acaggcgacg aaccagacgt
     5401 tgcgcgataa tccctgccag ggaagacgaa agcaggaatg attcgacgcc catgtcgcgg
     5461 agccgggtca ctgcgcctga tgcactgtta gtgtggagtg ttgagagtac cagatggccg
     5521 gtgagcgagg cctgaaccgc aatctgcgcg gtttctgtat cacgaatttc ccccaccatg
     5581 acgacatccg ggtcctggcg aagtatggcg cgcaggccgc gagcgaaaga catatccaca
     5641 cgggtattca cctgcgtttg cccaatgcct tccagctcat attccacggg atcttctacc
     5701 gtcagaatat tgcggccggg agtattcagc gccgaaagga tggcgtagag cgtggtgctt
     5761 ttaccggagc ctgtcggccc tgtcaccagg ataataccgt gcggaagctg aatgagattt
     5821 tctaaatcct gcttatccgc tgccgtcatc cccaggttgt tcaatgaaag ctggaggctg
     5881 tttttatcca gcaggcggag tacggcgcgt tcaccataga tggacggcag tgtggatacg
     5941 cggacatcta tgttacgtcg cccgatacgc aaactaattc ttccatcctg tggaatacgt
     6001 ttttcggcga tatcaagacg agccatgacc ttaattcggg agataagcag tgccgccagt
     6061 tttttgtttg gctgtaaaat tgtccgcaaa acgccgtcga tgcgaaaacg gatcgacatt
     6121 gttttttcat aggtttcaat gtggatatca gaggcggttt ctttgatggc ctcactcaaa
     6181 atcgcattga tcaagcggat aaccggtgcc gctgaatctt cattcaggag atcttcattt
     6241 gcgggcatct cttccgaaag cgagagaaga tcgacggatt gatcgatatc ctgcgcaatc
     6301 tgttgcgact caccggtatt ctgatggaat accgcttcca gccgagcttc aaaatcagcc
     6361 tgtgacaagc tggtcatggt ctggcactgg ccaacctgac gttgaatatc gagtaaccgc
     6421 tcgaatggcg tatcggcgag ataaataatc tcgccctctg aatagagata accaattcgt
     6481 tgtgccagcg cccaactggc ggggtacggt gagtgaattc tcatatgaat gcctcaccgt
     6541 gacgatggcg caggggcgtg actgttgaac gtgttttcat ccagcaccgg caaatcttcc
     6601 gagccaacca gcgcttttga tttcccgtcg atccgctgtt gttgctcctg acggtaacgg
     6661 gtgtatttct cttttgacag tgagcgataa acatcatcgt cacggataat cgtcggacgg
     6721 ataaatacca tcaggttgcg tttagcgcgc tcggtggagg tatagcggaa gagttgcccc
     6781 actaaaggaa tatcgccaag cagaggaacc tttgacactt gctctttaga aaaatcatcc
     6841 agcaatccgc ccaggaccac cgtttcaccg gttttgacca gcacggcgtt ttgaatagta
     6901 cgggtattaa acgtcgggcc gagcgtcgag ttagaggaag agtcaacgct ggagacttcc
     6961 tgctctattt cgagcaacac cgcgtcgcct tcattgacct gcggagtaac tttgagtttt
     7021 gtccccaccg ttttgcgttc gacggtatta aagacgttat cccctgaagt ggtctgtgac
     7081 ccggatagca ccggcacatc ctgccccacg ttgaaggacg cgagtttatt atccagcgtt
     7141 acgatgcttg gggtggcgag gatgtcattt ttattgttac tggctagcgc ggtaagcagt
     7201 actccccagt cgccattgaa gaagcctgcg gccatgccat tgtaggcgct aaacatatcc
     7261 caggcaggat tcgcgctggt gatcccacca ttctttttat aatcagccac accttgcgca
     7321 gcgttaaaaa tcggtaatcc ggtattggta aattgctgtg cgccaacgtt tttattcgcc
     7381 cattgcacgc cgaggtttag tccatttcca tcctgaactt caacgatgat tgcctcaacc
     7441 agcacctgtg cacggcgaat gtccagacgc gcaattaccg tggcgagttt ttcctggacg
     7501 gactggtcag cggtaatgac cagagagtta gtctgttcat cggcggtaat ggcgacgtta
     7561 tccatcgcgc cagaagagga gggcttacgc gcattccctt tttcatcttt cagcttttcg
     7621 gaaacaccgg ttagcacttc caccagattc gtggctttag catatttcag gtaataaacc
     7681 cgggtatttc cttcctcgct ctcttcgaca tcaaggcttt tcagtaatga agtgatgcgc
     7741 tggcgtgctt tttccggtcc actgatgata agagagttgg ttcgcttatc cgccacaatc
     7801 ttcgcggaga ggagggctgg catctggctt ttaccgtggc tttcgctgat taattgatta
     7861 agaatctcgg cgagatcttc cgctgacgca tattccagat gaataatttg ctgcttctct
     7921 gtgccgatga catcaacgcg ctttatgact tcaatcagtt tattaatggt ggaggcacga
     7981 ccggtcagaa taagaacgtt ggagggttca taatgcacaa cattaccgac gctacccgca
     8041 tccatcatct ggcggagcag gggggccagg tcacgagcag gaacgttctc aagcggtaca
     8101 attcgggtga ccaactcatc acctacgcct ggacgagaac tgtcagcaat catccctggc
     8161 gatgttttta cattagctga gcgaaccact ttcagaaaac cattgtccag cgtgatcacg
     8221 gaataaccgt aaagatcaag aatacttaaa aagaactggt agtactcctg ttggctaaac
     8281 gtatcattac tgcgtacgga aatggttccc tgtaccgaag ggtcgatcag gatcgttttg
     8341 ccaagatgct gacccactat ttccacgaac tggcggatat cggcgttatt gaagttcgcg
     8401 ccgtattgtt cgttctcagc gtgtcctgca caaggcatga gtagtgctgc cagcaagcag
     8461 caggtgattt tattgagtcc tttcatgacg cagtccattc attcctgtaa gttccccgac
     8521 ggaaacattt atcaagcggg gtacgccgtt gcgacgaatt gtaaactgag cactttgttg
     8581 agttagcagt aaacttaatg cctgtgaaac ctcatcaggg tgggtcagcg aaagattatt
     8641 gatcctgagg gcgatatccc ctggttgtag caagctggtg gtaaatgcat tgaggccctt
     8701 acgagagttc aatcgaagcc cgtatatctg ttctccatca cgtataggcg tggcgataat
     8761 ataatcagcg agtacatttt gactttctga atcaggctgc ttgatgatgt caggtagccc
     8821 tggatggaga gttacttttt cgtaatgccc gtgagtttca aacacaacgc tggttttatt
     8881 tatttttcta ataaacgttc ccggcgctga ctcaagggct tcgttgaggg agtatcgctt
     8941 ttgttcgctc ccctcattaa ttaagacaaa agaagtctca tcattcgatg tgagtacaat
     9001 gccattaagc gctatagaga gcttaggtgt ttcctgatta actgccacgg gatttgcatc
     9061 ttttacccca gcatgatttt
//
LOCUS       DBBGLQ010000146         8992 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.146, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000146 DBBGLQ010000000
VERSION     DBBGLQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.146"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..161)
                     /locus_tag="ACIYSB_004744"
     CDS             complement(<1..161)
                     /locus_tag="ACIYSB_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012304878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HGC5431620.1"
                     /translation="MYITLLFIQRVYFISSEKKLTIHIVQMFQLLSQAFYNLKMFLMM
                     DMLGVGDAI"
     gene            complement(253..870)
                     /gene="yncG"
                     /locus_tag="ACIYSB_004745"
     CDS             complement(253..870)
                     /gene="yncG"
                     /locus_tag="ACIYSB_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGC5431621.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRTERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCVMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            1136..2635
                     /gene="ansP"
                     /locus_tag="ACIYSB_004746"
     CDS             1136..2635
                     /gene="ansP"
                     /locus_tag="ACIYSB_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGC5431622.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIEFKPETAS"
     gene            complement(2748..3809)
                     /gene="yncE"
                     /locus_tag="ACIYSB_004747"
     CDS             complement(2748..3809)
                     /gene="yncE"
                     /locus_tag="ACIYSB_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGC5431623.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLVDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4051..6153
                     /gene="pqqU"
                     /locus_tag="ACIYSB_004748"
     CDS             4051..6153
                     /gene="pqqU"
                     /locus_tag="ACIYSB_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGC5431624.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLCYERSLSSRDDMSVMMYAGERETTQYQSIPMEPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYITPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            complement(6189..6854)
                     /gene="mcbR"
                     /locus_tag="ACIYSB_004749"
     CDS             complement(6189..6854)
                     /gene="mcbR"
                     /locus_tag="ACIYSB_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGC5431625.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(7052..8089)
                     /gene="curA"
                     /locus_tag="ACIYSB_004750"
     CDS             complement(7052..8089)
                     /gene="curA"
                     /locus_tag="ACIYSB_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGC5431626.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8269..8787
                     /gene="mddA"
                     /locus_tag="ACIYSB_004751"
     CDS             8269..8787
                     /gene="mddA"
                     /locus_tag="ACIYSB_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGC5431627.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTLAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8784..>8992
                     /locus_tag="ACIYSB_004752"
     CDS             8784..>8992
                     /locus_tag="ACIYSB_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGC5431628.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVR"
BASE COUNT         2170 a         2224 c         2208 g         2390 t
ORIGIN      
        1 ttaattgcat ctccaactcc gagcatatcc atcattaaaa acattttgag attatagaat
       61 gcctgtgata acaactgaaa catctgcaca atgtgaatag ttagtttctt ttcagaggaa
      121 atgaaataga cacgctgaat gaaaaggaga gtgatataaa ttaaaaaaac ataccatgaa
      181 tttaattaat tacgcccatc attgtggata aagttactga ataataaata acataatctt
      241 ctggtatccc actcaaataa tttcattcct ttttaatact tcttgtaatt tcggtaactg
      301 acatacagca tcagcaatcg cactgatatt cgtggcatta tcctgaaacc attcatgccc
      361 cggcccccat gtgcgcataa cgcagagata acagtccacc agggttagct gctcgccaaa
      421 cgcataaggt tcagcagtga gctgcgaatt caaccagata taaagcgatt ttcgatattc
      481 aataacattc ttctttaact gttcaggcgc gtcaggagcc caacgctcag ggtaatcggc
      541 gaaagtgaac gttggataga cattagcaac cagccagacc aataaccgtt gaaactgctg
      601 gcgttcggtg cgcccaaccg gtggcgcaag gtccgggcga cgatcgagaa ccatcaatgc
      661 aatcgccgct gtctccgtca taatctcgtc attttccagc gccagtgtcg gaacctgaca
      721 caacgggttc agggttttca ataactcgcg tgaagttccc tcgtggtcaa aaccactgac
      781 atcaacgaac tgataaggaa tatcagccag tgttagcatc agctcactga ttgtagagcc
      841 ccatccgggt acgccataca ccttaatcat atgcccctca caatctttta aagattaagt
      901 gtaggacagc acacaggtaa acctgcaaaa ggattgtcat tttttcgtca gttgtatgaa
      961 tcctcttcag gattgtaagg ggatgttttg catcgtttat cgataacggg taggatgctg
     1021 tgccgatagt tttataccca taaagaataa tggtgaatct tgtcatcgcc aggatgaata
     1081 aacattgttc atggcaactt atatgacttt ttcattaaag caatcaggga gagcaatgag
     1141 taaacacgac accgacactt cagatcaaca cgccgcgaaa cgccgctggc ttaatgccca
     1201 cgaagagggg tatcacaaag cgatgggcaa tcgccaggta cagatgattg ccattggcgg
     1261 cgcgattggc accggcttgt ttttaggcgc gggagcccga ctgcaaatgg cggggccagc
     1321 actggcactg gtctatttga tctgcggcct gttttcattt tttattctgc gtgcattggg
     1381 tgagctggtg ttacatcgcc cttccagtgg cagctttgtt tcttatgccc gtgagttttt
     1441 gggtgagaaa gccgcttacg ttgctggctg gatgtacttc atcaactggg cgatgacggg
     1501 gattgttgat attaccgctg tagcgctgta tatgcattac tggggggcgt ttggcggcgt
     1561 gccgcagtgg gtctttgcgc tcgctgcgct gaccatcgtt ggcaccatga atatgatcgg
     1621 tgtgaaatgg tttgcggaga tggagttctg gtttgcgctt attaaagtgc tcgccattgt
     1681 gacctttttg gtcgtgggta cagtgttcct cggtagcggt cagccgctgg atggcaacac
     1741 cactggcttt catttaatta ccgataacgg cggcttcttc ccccacggtt tgctgcctgc
     1801 gctggtgttg attcagggcg tagtgtttgc ttttgcctcc attgaaatgg tgggtacagc
     1861 tgccggagaa tgtaaagatc cgcagaccat ggtgcctaaa gcgattaaca gtgtgatttg
     1921 gcgtattggc ctgttttacg tcggctccgt ggtgttgctg gttatgttat tgccgtggag
     1981 cgcgtatcag gcggggcaaa gtccgttcgt gacgtttttc tctaaactgg gtgtgccgta
     2041 catcggcagc attatgaaca ttgtggtgct gaccgctgcc ctctccagcc tgaactcagg
     2101 tctgtactgc accggacgta ttctgcgctc gatggcgatg ggcggttccg caccgagttt
     2161 tatggcgaaa atgagtcgtc agcatgtgcc gtatgccggg attctggcga cactggttgt
     2221 gtatgtcgtc ggcgtattcc tcaactatct ggtgccgtcg cgcgtatttg agattgtgtt
     2281 gaacttcgcg tcgctgggaa tcatcgcttc atgggcgttt atcatcgtgt gccagatgcg
     2341 cctgcgtaaa gcgattaaag aaggcaaagc agcggatgtc agttttaaac tgcctggcgc
     2401 gcccttcact tcctggctga cattactgtt tttactgagt gtccttgtgc tgatggcgtt
     2461 cgattacccg aacgggactt atactatcgc ggcgctgccg attatcggta ttctgctggt
     2521 tataggctgg tttggtgtgc gcaaacgtgt tgctgaaatt cacagcactg cgccagtcgt
     2581 cgaggaagat gaagaaaaac aggaaattga gtttaaacct gaaacggcga gttaattgca
     2641 aaattgcccg gctacgtgcc gggcaaattg aaggtgctct ttacttcatg ccggatgcgg
     2701 cgcaagcgcc ttatccggcc tacgaaagac aaactccccc atatctatta cagcgcaata
     2761 cgaatcacat cgtccggctg ggtagcttcc tgctgtttag tggatttttg tttcacactg
     2821 acatacaacg ttttgccatc ggcagacagc gccaggctgt tcggatgagt cggcgtatcg
     2881 aacgttttca ccactttata gcttttcgcg tcaatcacac tgactttacc tgcctgacga
     2941 tgcgttacgt aagcttcatt acgcgcgggg ttaaacagca cagccagtga ttccggtgcc
     3001 gcaaccttcg ccagaatatt gccattacgg gtatcgacca ctaacacttc tgccgcttta
     3061 gaatcggtga taaatgcacg ctgcctggcg gtatcaaggc tgatgttgat aaagaagtgc
     3121 tctttgccgt catccaccag ctttttacgg ctgaggattt tattgtcggc ggtgtcgatg
     3181 gtaatcaatt cgccgtcagc gttagtggtg taaagacgtt tgcctttgct atccaacgcc
     3241 agaccggtac tcattttacc ggtgttctgg atggcggttt tcagtttgat attctcgcca
     3301 tcaacgaccc aaatcacgct ctctttacca ataccactga tgtaaacggt gttcgtggca
     3361 tcgtcagcta ccagctcacg cggttgcagc gggcgcacct cttccgtgcg cttacgatca
     3421 tccagcacca gacgaccttt cacctcgccc gttttggcat ctatcgccgt gaccgcactg
     3481 tttacggtgt taccaaacca caacgtctga gtcgtgttat tgatggtggc accaaacggc
     3541 ttgagatcgt tatggatcgc ctgcgtcact tccagagtga ccggatcaag acgataaacc
     3601 acaccacctt tatccagttt gcggctttgc gaagtggcga gccacagcgc gttttcttgc
     3661 tggctataag ccatttcgta ggcaccttta cctaccgctt tacgcagcat ttcttctgcg
     3721 gcctgcgtac tgaatgatga agcaacaagc aatgaaccta acagtaatga accacgcagg
     3781 cgcgatgaaa acagatgacg taaatgcatg acgactccct ttgatgaacc gatatttttg
     3841 ctgcttcaca atgcttcgct aagcgtaatc atcgttacgc tcttattata aatgggaatg
     3901 gtaatcatta ttttctcaaa tgtggagcat ttcttgccgt tacgactaca attttctcaa
     3961 ttacttaacg tatcgtgccg tttcgttttc cataatttta caatttcatt accataacgg
     4021 cgttttccca ttcttaacct ggcttttttc atgaagattt tttccgtccg acagaccgtt
     4081 ttgcccgcac tacttgccct ttcccccgtt gtttttgccg ctgatgaaca gaccatgatt
     4141 gtcagtgccg caccgcaggt ggtttcagaa ctggataccc cagcagcagt aagcgtggtg
     4201 gatggcgagg agatgcgcct ggcaacaccg cgcattaact tgtccgaatc actgaccagc
     4261 gtgcctggtt tgcaggtaca aaatcggcag aactatgcgc aagatttaca gctgtcgatt
     4321 cgcggatttg gctcccgctc cacttacgga attcgcggta ttcgcctgta tgtggacggt
     4381 attcccgcta ccatgcccga cgggcaaggg caaacatcca acatcgattt aagcagtgtg
     4441 caaaatgtgg aagtgctgcg tggccccttc tctgccctgt atggcaacgc gtctggcggt
     4501 gtaatgaatg tcaccaccca gaccgggcaa cagccaccaa ccattgaagc cagcagttac
     4561 tacggcagtt ttggcagctg gcgctatggg ctcaaagcaa cgggtgcaac gggcgacggc
     4621 acacagcccg gcgatgtcga ttacaccgtc tcaaccacgc gttttacgac ccacggctat
     4681 cgtgaccata gtggcgcaca gaaaaattta gctaatgcca aactgggcgt acgcattgat
     4741 gacgccagca aattaagcct gattttcaat agcgtggata tcaaagcaga tgaccctggt
     4801 gggctaacca aagcagaatg gaaagcgaat ccgcaacaag cgccacgcgc tgaacaatac
     4861 gacacgcgaa aaaccatcaa gcaaactcag gctgggttgt gctatgaacg tagcctgagt
     4921 tcgcgggatg atatgagtgt gatgatgtat gccggagagc gagaaacgac ccagtaccag
     4981 tcaataccca tggaaccaca acttaacccg tcacatgcgg gcggcgtgat taccctgcaa
     5041 cgccattacc agggaataga cagccgctgg acacaccgtg gtgaactggg cgttccggtc
     5101 acgttcacta ccggcctgaa ctacgaaaac atgagtgaaa accgcaaggg ctacaataac
     5161 ttccgcctga atagcggcat gccggagtac gggcaaaaag gtgagttgcg tcgcgacgaa
     5221 cgcaatctga tgtggaacat cgatccctat ttacagacgc agtggcagct gagcgaaaaa
     5281 ctgtcgctgg atgctggcgt gcgctacagc tccgtgtggt ttgattccaa cgaccattac
     5341 attactccgg gtaacggcga tgacagcggt gatgccagtt atcacaaatg gctaccagcc
     5401 ggatcgttaa aatatgcaat gaccgatgcc tggaatatct atctggcagc cgggcgtggt
     5461 tttgaaacgc cgacgattaa tgagctgtct tatcgggctg atgggcaaag cggtatgaac
     5521 tttggtttaa aaccatccac caacgataca attgagatcg gcagtaaaac gcgtattggt
     5581 gatgggctgc ttagtctcgc attgttccag accgacaccg atgatgaaat tgttgtcgat
     5641 agcagtagcg gtgggcgtac gacttacaaa aatgctggaa agacccgtcg tcaaggcgct
     5701 gaactggcat gggatcaacg tttcgcggga gattttcgcg taaacgcgtc ctggacctgg
     5761 cttgatgcga cctatcgcag caatgtttgc aatgaacagg attgtaacgg taatcggatg
     5821 cctgggatcg cccgtaatat gggctttgcg tcgataggtt atgtaccgga agatggctgg
     5881 tatgcaggca cggaagcgcg ttatatgggc gatattatgg cagatgatga aaatacggcc
     5941 aaggcgccgt cttatactct catcggctta ttcaccgggt ataaatacaa ttaccacaat
     6001 ttaactgtgg atttatttgg tcgtgtcgat aatttattcg ataaagaata cgttggttct
     6061 gtcattgtca atgagtcaaa cgggcgatat tacgaacctg cgcccgggcg aaattatggt
     6121 gtcggcgtga atatcgcatg gcgatttgag taattacctt gatgcccggt atttgccggg
     6181 catttacttt aacgattgta ttgctggtat aaaatagcaa tattttgttg cataatttca
     6241 gcaagacaat gtctgcatcc ctctttgtct tttgctttta acgcagcgag taacagatga
     6301 tagttttcta tatgctcccg taattccgct ggattaatcg cttcatagag ataatgtaaa
     6361 ccaggtccca tcctgaccca cagttgctca atcatctcac acaggatggg catatttgag
     6421 cgatgataaa tcgctaagcg aaatagcctg tttacattaa tgatttgttc catgtcgccc
     6481 ttttcttgcg cctgctgtaa tttctcgagc aattcctgga gttccgccag gtcttgcggg
     6541 gtgaggtttt caacagccag agcaactgcc attaattcca gttcgtagcg gatccgattg
     6601 atttcgtcta attgacgttt ccccacttcc ggaactgtaa atgcttgcgc tggtgcgaca
     6661 gaaagcgcat tcaccgaaac cagacgtaat aatgcttcac gcacaggtgt aatactcata
     6721 cctaattgct ccgccagatt tttagtaatc aggcgtgcgc caggttttag tgcgccaata
     6781 ctaagctgat gtttcaggtc gttctcaacc tgcagagtca aactgacatg tttcattttt
     6841 tccgttccag gcattttaat tccttcgcgt gttttctgtc caaccctgcc tgcatggcag
     6901 aaggaattca cctggtgtat taaagtgaat aatcataaac cactcacctt tgttgttatt
     6961 agcagcagaa caatgatgtg gttagttaat taaagctgaa aataagcttt ttgcctggat
     7021 ttttatggcg gcttacgccg ccgttgaaac tttaatcatc acccgccacg cggatcacca
     7081 ctttgccgaa gtttttaccc ttcagcaggc cgataaacgt ctgtggcgca ttctctaaac
     7141 cgtcagtaat ttcttcgcgg tagtggattt tatcctcttt cacccattgc cccatctccc
     7201 tctgaaactc atggatgcgg tgaccataat cctgagcgat aataaaacct tgcaagcgaa
     7261 tacgtttttt cagcactgta gccatcaaca gaggtaaacg atccggaccg ggtggtagct
     7321 ctgtagcgtt atagctgctc actaatccgc agacgggaat gcgcgcagat gtattaagta
     7381 atggtagcac tgcatcaaat accttaccgc ccacgttttc ataatagata tcaatacctt
     7441 ttgggcacgc tttcgccagt tgttcggcaa aatcatccgc gtggtgatca agacaaacat
     7501 cgaagcccag cacctcggta gcatggcggc atttttccgc gccaccggca acccccacca
     7561 ccctgcaacc tttaagtttg ccgatttgcc ccaccgtcgc ccccactggt cctgtcgccg
     7621 cagctaccac cagcgtttcg ccctctttag gctgaccgat atccagtagg cccatataag
     7681 cggtaaagcc tggcatcccc agcacaccca gcgaccacga tggattttgc ggatgatcgc
     7741 caagtttcac cagatcatca ccactggata tgtcatagtc ctgccatcca ctgtagccca
     7801 gcacccagtc gccaggctga taatcaggat gattcgactc cacaacacgg ctcaccgtgc
     7861 cgccgaccat cacgccgcca atatcaacag gcggtgaata agatggctca tcgctcatac
     7921 gtccacgcat atacgggtcc aaggacaaat aaactgtgcg cagtaacacc tgtccttcac
     7981 ccggtgtggc gacatcatct tcttcaagac ggaaattctc cggaaccggt gcgccatgtg
     8041 gacgcgaggc cagaacccaa cgtcgattac gctgcttttg ttgccccata atgctctcct
     8101 gtgcttttgg catgaatcca tcaatagtag caggctcacg ggattggttt tgtttgcagc
     8161 cgtcacatcc cctctgaaca cgcaataatt tcatcgagga atccgttgct tcgggctgtt
     8221 atcgatagta ttgataacat catcatcatt attttatcga ggccagacat gtccatccgt
     8281 tttgcccgca aagccgattg tgctgctatt gcggaaattt ataaccacgc cgtgttgtat
     8341 acggcggcta tctggaatga ccaaacggtg gatgcagata accgcattgc ctggtttgaa
     8401 gcgcggactt tagctggtta tccggtgctg gtgagcgaag aagatggcgt agtgacggga
     8461 tatgcctcgt ttggcgactg gcgtagtttc gatggttttc gccataccgt ggaacattcg
     8521 gtttatgtcc atcccgatca tcagggcaaa ggtctggggc gtaaattgtt aagccgattg
     8581 attgatgaag cgcgggattg cgggaagcat gtcatggtcg ccgggatcga atcgcaaaat
     8641 caggcctcgc tgcatctgca ccagtcgctg ggatttgtcg tcaccgcaca aatgccgcag
     8701 gtgggcacta aatttgggcg ctggctggat ctgacattta tgcagttgca actcgacgaa
     8761 cgcactgaac cggacgcgat tggatgaatc agtcgctcac ccttgccttt ttgattgccg
     8821 ccggaattgg tctggtagtc cagaacacgc tgatggtgcg catcacccag acctcctcta
     8881 ccattctcat cgccatgttg ctgaactcac tggtggggat tgtgctcttt gtttctattt
     8941 tatggtttaa acaaggtatg gcggggtttg gcgaactggt gtccagcgtg cg
//
LOCUS       DBBGLQ010000147         8908 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.147, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000147 DBBGLQ010000000
VERSION     DBBGLQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.147"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            295..456
                     /locus_tag="ACIYSB_004753"
     CDS             295..456
                     /locus_tag="ACIYSB_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGC5431629.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARF
                     IQMLTEFLK"
     gene            complement(648..785)
                     /locus_tag="ACIYSB_004754"
     CDS             complement(648..785)
                     /locus_tag="ACIYSB_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HGC5431630.1"
                     /translation="MRKRFFVGILAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISV
                     Q"
     gene            833..1174
                     /locus_tag="ACIYSB_004755"
     CDS             833..1174
                     /locus_tag="ACIYSB_004755"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HGC5431631.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIV
                     ISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLGLFISIKNN"
     gene            complement(1171..2736)
                     /locus_tag="ACIYSB_004756"
     CDS             complement(1171..2736)
                     /locus_tag="ACIYSB_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HGC5431632.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIE
                     KEQAETERQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRL
                     SSSIHARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRR
                     TGAGKIMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKT
                     AQTNLLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAF
                     EKYKDVLNKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDI
                     LKIKDTGDWKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFID
                     KNKLNTINDVLGI"
     gene            3014..3433
                     /locus_tag="ACIYSB_004757"
     CDS             3014..3433
                     /locus_tag="ACIYSB_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HGC5431633.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETS
                     ELIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATE
                     RRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
     gene            3517..3936
                     /locus_tag="ACIYSB_004758"
     CDS             3517..3936
                     /locus_tag="ACIYSB_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HGC5431634.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
     gene            4462..4638
                     /locus_tag="ACIYSB_004759"
                     /pseudo
     CDS             4462..4638
                     /locus_tag="ACIYSB_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6517..6750)
                     /locus_tag="ACIYSB_004760"
     CDS             complement(6517..6750)
                     /locus_tag="ACIYSB_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HGC5431635.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
     gene            complement(6757..7275)
                     /locus_tag="ACIYSB_004761"
     CDS             complement(6757..7275)
                     /locus_tag="ACIYSB_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HGC5431636.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSN
                     ETKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     PFQGKEWVHLKE"
     gene            complement(7313..8011)
                     /locus_tag="ACIYSB_004762"
                     /pseudo
     CDS             complement(7313..8011)
                     /locus_tag="ACIYSB_004762"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7953..8276)
                     /locus_tag="ACIYSB_004763"
     CDS             complement(7953..8276)
                     /locus_tag="ACIYSB_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HGC5431637.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            8341..8532
                     /locus_tag="ACIYSB_004764"
     CDS             8341..8532
                     /locus_tag="ACIYSB_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HGC5431638.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT         2357 a         2345 c         1841 g         2365 t
ORIGIN      
        1 aaaaaaaacc agggacagga agaaggccgc gccgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa attgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 taaagatacc aggcgtttcc ccccggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgtt ggtatctcgg cccggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgccttaac gccactggca gcagccactg gtaaccggat tagcagagcg
      421 agatttatcc agatgcttac agagttcttg aagtgatggc ctaactacgg ctacactgga
      481 aggacagtat ttggtatctg tgctctgctg aagccagtta cctcggttaa gcagttcccc
      541 aactgactta accttcgatc aaaccgcctc cccaggcggt tttttcgttt acaggcagca
      601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaacagag
      661 atccccgtca gtttagaaga agatgatgga gccacagtcc ctccctgaac atcacgtata
      721 tagttagcct gacatccaac aaggaggttt atagctaata ttcccacaaa aaatcttttc
      781 ctcataactc gatccttata aaatgaaaag aatatataac gaggtttaat ttatgagttt
      841 aagatactac ataaaaaaca ttttgtttgg cctatattgc acacttatat atatatacct
      901 tataacaaaa aacaacgaag ggtattattt cctagcgtca gataagatgc tatacgcaat
      961 cgtgataagc actattctat gcccatattc aaaatatgct attgaacaca tattttttaa
     1021 gttcataaag aaagattttt tcagaaaaag aaaaaatcta aataatgccc ccgtggcaaa
     1081 attaaaccta tttatgctat acaatctact ttgtttggtc ctagcaatcc catttggatt
     1141 gctaggactg tttatatcaa taaagaataa ttaaataccc aacacatcat ttatagtatt
     1201 aagtttattc ttgtcaataa aggcacatag aatgcctgta acaatagcaa tcccccagat
     1261 acctaatgta gttccagcaa gcacactaaa aagtaaaaca acaatataac taactccagc
     1321 atctacagtt ttcttctcta atgtaagaaa tagtggcttc cagtcacctg tatccttaat
     1381 ttttaggata tcagatacca catcatatcc aaaagaaaca tgtcccgtaa tcttcaagta
     1441 cttagcaaac tgatctaaat gcttagccca gtcttcatac ttaaccgatt ccagtgcatt
     1501 gaaaatcgca tcacggtctg ctttgctgaa tttcttattt aaaacatcct tgtatttttc
     1561 aaaagcagct agagcttcat tcacattgct gattttctta cctttagact tatcagcaag
     1621 ttcctgtgcc atttttgaat atttttcacc atatttttca gatagcgttt gataaaagct
     1681 aactgttgca tccacagcat ccttaatctg cgagttcagg agattagtct gcgctgtttt
     1741 caaattttct tcagattcat gaacacgtaa aacagctgta ttacgattat tattagcctg
     1801 agacatagcc ccctgaattc catttatctc agcattaagt tggttaatac gcgtttctga
     1861 tgctgttacc tgtttttgtt tttcctccat aatcttaccg gctcccgtcc gtcgactggt
     1921 ggcatagaaa aaaggacgac tctgaagcgg atcattagct cttggtgaca attttttgac
     1981 cagttcatca agttccttat atttagcaga tgcctgagcc agctcatttc gttttccgga
     2041 cagcgtattc atttctgcat cacgggcatg aatactggag cttaaacggg tattgagagt
     2101 cttaatctct tcatccactt tcgccagctc agattgtgca gcagcaagat ttttttgtgc
     2161 cacctccact gcccgggcct cttcattcaa tgctgccagc cgtttctctt cagcttcagc
     2221 cagtttcaac tggcgttctg tttcagcctg ctccttctct atctctttac gtcgttgttc
     2281 tgcctcctga aaagcctttt ccgcagcttc cgcttcttta cgggcttttt cttctgcttt
     2341 tgcaaggcgc aaccgctctg cttctgcctg catcgctgca ttattggcat gagccaggtc
     2401 aataacctcc ggatgagtgg aattatgacg aagcgcctca ttcacaatat ccttcagatg
     2461 ttgagtcagc gcatcccggt ttgcttttgc tttagcctgt gcttctgcgg cagcttttgc
     2521 ccgggcagcc tgttctgcct gcgttttctt caattgagca gtagaccatt tagctgtggc
     2581 atgaatggct gcagaacttt cacttttgct acctccagtt ccaccaccgc cgccagagcc
     2641 actcccgtct ggattaccat tcaaaagagt aatgattacc tgccccttat catcataagg
     2701 aacaccatct ttatagtacg ctacagctgt ttccattata aaatcctctt tgacattaaa
     2761 aacaaatagt taaaagcaat acactgtata tataaccact ggttttatat acagcttaaa
     2821 attacgccgc tgcattttcc ctgtcaaccc tgtggatttt catttttgtg aaaacgatca
     2881 aaaaaacagc cacccacacc acgtgctatt cccacccgat gccacaaaaa ccagcacaaa
     2941 cattaccgtt ctccggcccc agtatgtttt actgaaacca tgatatgaga cattaatggg
     3001 acactgtcac ttcatggcat ggcacacact ccagaacgca ctgaaaatga caggcagatc
     3061 gcgttcacag ttttaccgtg atatgcgcgc aggccttgtc agttatcgta caggccagga
     3121 cggacggcgg gagtttgaaa ctagtgaact gatccgggca tacggcgaat taaagcagga
     3181 tgagacacca gaaaggcaca gtgcgggaca ggcagaaaat ccacatgatc agcagacaga
     3241 acgcattctc cgggagctga atgagctgag acaatgcctg acactgatgc ttgaggacaa
     3301 acaggcacag gctacggaac gccggcgcca ggaagccgaa cgggaacagc tacataaaga
     3361 gatagagcag ctcaggcagg cactggagca ggaaaaaaaa cggggattct gggccagact
     3421 gttcagccgg tgagcaccga ctgcaagtga tgataagata gtcagcggtt aaatataagt
     3481 cactgagtac aaagatttaa aggtaaggag acactcatga gacgttccgg cggcacactg
     3541 tatcttatcg ccctgctgac agcggcaacg gtcctgacag cctgcacgcc aaagggcagt
     3601 gtggaacaac atacccggca ttacgtttat gcatcagacg atggttttga tcctaacttt
     3661 tccacccaga aggccgacac aacacgaatg atggtgcctt ttttccggca gttctgggat
     3721 atgggagcaa aggataaagc gacaggaaaa tcacggagtg atgtgcagca acgcattcag
     3781 cagtttcaca gccaggaatt tttaaactca ctccggggca caactcaatt tgcgggtact
     3841 gattaccgca gcaaagacct taccccgaaa aaatccaggc tgctggctga gacgatttct
     3901 gcggtttatc tcgatggcta cgagggaaga cagtaagtgg atttaccata atcccttaat
     3961 tgtacgcacc gctgaaatgc attcagcgca atcacggcag cagacaggta aaaatcgcaa
     4021 caaccccccc gaaaaactac cgcgatcgcg cctgataaat tttaacctta tgcataccta
     4081 tgcatccagg tgaatcacaa gcgaatttcc ttactgagac gactgaaacg gttttttttc
     4141 acaattttca gctgttttcg attccacaaa atagaagtta caggataggg attaatcccc
     4201 ttcgccgcca aggggagcag gatgagtcag gggaatgtct attcacctgc gctgtacagg
     4261 ctgccaggta aaagtattcg ctgcggcacg atactctttt gccctcatag cctttgcgct
     4321 tcttactttt cgttcttcca gaatcattct taagacattt tcccgcactt ttctctaact
     4381 tcccgtggca atttatctct gccggactga accgccccgg aaatcctgga gactaaactt
     4441 cctgagaaag atgtaaacag gatgactaaa aatactcgtt tttcccccga agtccgtcaa
     4501 cgggcagttc gtatggttct ggaaagccag ggcgaatatg actcacaatg ggcggcaatt
     4561 tgttccattg ctccaaagat tggctgtacg ccagagaccc tgcgtgtctg ggttcgccag
     4621 catgagcggg ataccgggta ttacctttcg aagaaatttt tgacatcctc tacgccctga
     4681 aggaccgggt tttacggcgt accagataaa aaaacccctc tactgaagag gggtagtgta
     4741 aggaacttgc tgattttctt gatattatta accatcaata actgagcata caaaaaataa
     4801 caaaactctg acacagatac aaccatccct ctttgtataa gaccgatgtg catctcaaaa
     4861 aagaagcgtg tttctggaac acagaacaac aatgcacatt gtagccaaca aattcacacc
     4921 agaaacacgc tctgtttttt aatttacaca agaatatatg tctggaaaat caggaagaag
     4981 aaattaatgg gcattctgta tacgaaggga gagcgcgttt ccggtgcccc atattagata
     5041 ttatccctgc aacaaacacc agagacgcgc tcataattct tctagcgtaa atgatccttt
     5101 caggcaagaa aaaagccgtg attctgtatc acggccttaa ttttatcgaa aacacggatt
     5161 ttagtatgca catgactgta ctcctgtgga gtacaaggaa atcataatca gcgaccaaac
     5221 catttatcaa cagaaaacca acatattgat gttatttttg tgacaaatgt tacaaattcg
     5281 aaaccactgt atttttactg caacaaaatc tggttgatct acaagttaca ctggggtaaa
     5341 aagttgtccg gaatgtttct gcgtagtaaa gcctctagcc ggattgcctt tgcaaaaacg
     5401 caggaacgtt ccccccttca tcagtactcc tgacgctgta aggagctgac tgatgcagac
     5461 agggctgatg atatctaata aaagctatct catttttgcg cgtacataaa ataaatcagg
     5521 cacttcccta cactccccct ccggaattga gggggttttc tttacaatgt aaataaattg
     5581 cttgcgtaaa gttcaaaaaa actgcaaatt tttactgtag tgtcgagctt catgtagctc
     5641 accagggcat ggactaaaaa gaagtaaacc catcttttac cccccttata cggggggttc
     5701 tttatagcac aaagaacact tataatttat catctcacag ctataaatat aaaagaataa
     5761 tattctaaac tacagttatt tatgcagaac ataacatcaa actcacaata aatataaaac
     5821 aattatttta taactgttca cttattatat tcagacatat cagaaacacg actcaattct
     5881 tttttattta acaaaatcca taaaaaactt atggttgaaa tcacatttaa actgtgaagg
     5941 atatcatccg gagaaatttt tcaatccgac actccataga atggaccttt aagactttat
     6001 cctcatcatg tatgaggatt ttttttctgg caaacagtat ctttcagtaa tccttgctca
     6061 gttccgattc tgacgtatcc ccgtgtggat ctgaaaagga gataattacg ctctgtggcc
     6121 aacaggaata actcaagcag ctcctctctg ataccatcag aagaagcatt aaacgcattc
     6181 tggcgtcgat tacagaagaa gcggaacgcg ttccaggctc ctgttgctat acacactacc
     6241 cacactaacc ccatggtttt tgagcgccag aaacgcgtcc gtatgcgttt taacgcgttc
     6301 ctgtgttttc agcaaggcct gactgaagct agttcctagt gccctgaacc aaccattatc
     6361 caaaaaaatg caccggaaac gcgctgacaa cactttagcc tgtctttggt taccatccca
     6421 gcgacgggcc atccagtgtc cgttcctgta tttccagatg gcgctcacgc tctaggaata
     6481 gctcatgttc acgttcctgt atcagtagtt cgtcccttat ctcctgctct cgctgtataa
     6541 ctggctcaag tgttctgtct gctcgctcaa gtgctgcacc tgctgactca actgcatgac
     6601 ccgctcgttc agcatcgcgt tgtcccgttg cgtaagcgaa aacatcttct gcaattccac
     6661 gaaggcgctc tcccattcgt tcagccgctg catatagtcc tgttgcagct gctctaaggc
     6721 gttcagcaaa tgtgtttcca gctctgtcac tctgtgtcac tccttcagat gtacccactc
     6781 tttcccctga aagggaatca cctctgctga tttcccgtac ggaaggacca gaaatttcct
     6841 gttcccgtcc tgcacgaact ccacgcccca tgtcttcgcg ttcagtttct gcaatgtctc
     6901 ttcctgcttc ctgatatctt ccaggttcgc ctgtatcctc ccgccaagat accagagcgt
     6961 cccgccactc gcggtaaaca ggagaaagac tatccccagt aacatcatgc ccgtattccc
     7021 tgccagcttt aacacgtcct tcctgtgctg cctcatcgcc tctttcaccc cttcccggtg
     7081 tttctccagc gattcctctg tcgaggctgt gaacagggct atagcgtctc tgattttcgt
     7141 ctcgtttgat gtcacagcct cgcttacaga ttcgccgagc ctcctgaact cgttgttcag
     7201 cactttctct gtagattcgg ctctctcttt cagctttttc tcgaactccg cgcccgtctg
     7261 taaaagattg ctcataaaac gctcctttca gcctgatatt cttcccgccg ttcgggtctg
     7321 caatgctgat gctgcttcgc gtcaccctga ccacttcaag ccccgcctct gtgagcgcct
     7381 gaatcacatc ctgacggcct tttatctccc ctacatgata aagagcgtct atcccgcgtg
     7441 tgacgctctc agcaagcgcc tgtttcgttt ccggcaggtt atcagagaga gtcagtatcc
     7501 ggcggttctc cggggcgttg gggtcatgca gcccgtaatg gtgattcacc agcgtctgcc
     7561 aggcatcaat tctcggcctg tctgcccggt cgtagtacgg ctggagacgt tttccggtct
     7621 gtagctccat gttcggaatg acaaaattca gctcaagccg tcccttgtcc tggtgctcca
     7681 cccacaggat gctgtactga ttcttttcga gacctggcat cagtacgcgc tcaaagctcg
     7741 ccatcacttt ttcacgtcct cccggcggca gctccttctc cgcgaacgac agaacgcctg
     7801 acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc ccggacttct tccggattac
     7861 cctgaagcac cgttgcgcct tcgcggttac gctccctccc cagcaggtaa tcaaccggac
     7921 cactgccacc accttttccc ctggcatgaa atttaactat catcccgcgc cccctgttcc
     7981 ctgacggcca gacgcagccg gcgcagctca tccccgatgg ccatcagtgc ggccaccacc
     8041 tgaacccgat caccggaaga ccactgcccg ctgttcacct tcctcgcggt ctgattcagg
     8101 ttattcccga tggcggccag ctgacgcagt aacggcggtg ccagtgtcgg cagcttcccg
     8161 gaacgggcaa ccggctcccc caggcagacc cgccgcatcc ataccgccag ttgtttaccc
     8221 tcacagcgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
     8281 tcgcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca
     8341 gtgactaaac aggaaaaaac cgcccttaac atggcccgct tcatcagagg ccagacatta
     8401 acgctgctgg agaaactcaa cgaactggac gcggatgaac aggcagatat ctgtgaatcg
     8461 cttcacgacc acgccgatga gctttaccgc agctgcctcg cacgtttcgg ggatgacggt
     8521 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgtg agcggatgcc
     8581 gggagccgac aagcccgtca gggcgcgtca gcaggtttta gcgggtgtcg gggcgcagcc
     8641 ctgacccagt cacgtagcga tagcggagtg tatactggct taagcatgcg gcatcagtgc
     8701 ggattgtatg aaaagtgcac catatacggt gtgaaatgcc gcacagatgc gtaaggagaa
     8761 aatgcagctt ccggcgctat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     8821 ggctgcggcg agcggtactg actcacacaa aaacggtaac acggttatcc acagaatcag
     8881 gggataaggc cggaaagaac atgtgagc
//
LOCUS       DBBGLQ010000148         8785 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.148, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000148 DBBGLQ010000000
VERSION     DBBGLQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.148"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..463
                     /locus_tag="ACIYSB_004765"
     CDS             <1..463
                     /locus_tag="ACIYSB_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGC5431639.1"
                     /translation="ELCALRWSDIDFIGHTVHVQVASVVGVIKGTKTKAGTRKVELNS
                     EALMALQSQKSFTMMRSAFVFSDPKTRKPWANSAAIRKKAWIPTLKKAGVRYRNPYQT
                     RHTFATRHISQGANLFWLAGQMGHKGPEMLFRHYGSYLSEYDGNTAISEVI"
     gene            complement(783..5362)
                     /gene="ypjA"
                     /locus_tag="ACIYSB_004766"
                     /pseudo
     CDS             complement(783..5362)
                     /gene="ypjA"
                     /locus_tag="ACIYSB_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
     gene            complement(5427..5546)
                     /locus_tag="ACIYSB_004767"
     CDS             complement(5427..5546)
                     /locus_tag="ACIYSB_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGC5431640.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(5770..5928)
                     /locus_tag="ACIYSB_004768"
                     /pseudo
     CDS             complement(5770..5928)
                     /locus_tag="ACIYSB_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(6274..7647)
                     /locus_tag="ACIYSB_004769"
                     /pseudo
     CDS             complement(6274..7647)
                     /locus_tag="ACIYSB_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
     gene            complement(8398..8473)
                     /locus_tag="ACIYSB_004770"
     tRNA            complement(8398..8473)
                     /locus_tag="ACIYSB_004770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8438..8440),aa:Ile,seq:cat)
BASE COUNT         2316 a         2104 c         1741 g         2624 t
ORIGIN      
        1 agaattatgt gcattacgtt ggtctgatat tgattttatt ggtcataccg ttcatgtaca
       61 ggttgcttct gtcgttggtg tgataaaagg aacaaaaaca aaagctggta caagaaaagt
      121 tgaattaaac agtgaagccc taatggcatt acagtcacag aaatcattta cgatgatgag
      181 aagtgctttt gtattcagtg acccaaagac acgcaaaccg tgggctaatt cagcagcgat
      241 tcgtaagaaa gcctggatcc cgactcttaa aaaagctggt gttcgttacc gtaatccata
      301 tcagaccaga catacattcg ctacacgaca tatcagccag ggggcaaatt tattctggct
      361 tgcaggccaa atggggcaca aaggacccga aatgcttttt agacactatg gttcatattt
      421 atcagaatat gatggtaata cagcaatctc agaagtaatc tagtcgctta aatatttcaa
      481 aatatgtaca aaggtaggac acagaataga ccttaactta cacctgaaat acataaaaat
      541 atacagtgaa atatgaaatc atttgtattt catattgtta tgtaatttgg gtgtttgggt
      601 tcaactcccg ccagctccac caatcatgat tggacggtgt aaggacaaca ccaacaaaaa
      661 caggaagtta gaagtctcag caaaacaccg accagacggt gaggagacat aaaaggatac
      721 gcaaaggagc cgcggctcct ggtaacatga aagcccacag atgtgggctt tttcgttgat
      781 ggtcagaacg accagttcac accagctacc gcgttccacg gggattccac accggcacta
      841 tggctatacc ccaccccaag atgcccgctt aacgtactgc tgaatgaggc tttaatacct
      901 gcctggtata ttccacgtct gcccgacaaa taattgacga aattaccgtc actattcact
      961 ttcacccggt tatcatcgac aaattctttg cgcacagccg ccttcagcca cggctcaact
     1021 tccataccgt tccccagacg catgttgtaa ctcagcgttg cgcccagttc acgatatata
     1081 ctgcgggtat cgactgattt cgatttcatg ccattggata aatgatattc ggggttatca
     1141 gcggtgaacc ccgttaacga tgcatacggc gtcaggttcc agttaccatc ggtaaatcgc
     1201 atcccggttt caatgtgacc gcccagcccg ttgctgtggt aactgccatt ggcggctcca
     1261 ccgctgctca ttttacctgc tacgttactt ttaaaacggt tcagcttcac gacaccgtcc
     1321 agatagaaac cactttcatg ttcccaactg gcatagccgc ccagagaata actgcccaca
     1381 ctgccatgtc ctccgcgatc aaaaccgata tgtgaatggg aatagcccat aaaagcgcct
     1441 agcgtggtaa ttccttcagg aatatcatta cggctgtcga tccccactgt cattccggtc
     1501 agcgtctgct caaacccggc acccgcatcg gtggtgacat tattacgggt gttatacgtc
     1561 gccccccaga cattattgtt gtgtggactc gctttcatta tgttcaaccg ctcgcgaata
     1621 ctgtttagct cagcatcaaa taccaacggt aatgttgctg ccatattgag tacggctgcc
     1681 gtagaaggcg taatgcgttt ctccggaacg ggtgtcggcg ttggatcggg agtagggtct
     1741 ggttttggat ttgggtctgg ctttggatcg ggttttgggt ctggctttgg atttgggatg
     1801 gggtccgggt tgggtttgac atcattggtc aggttccagt tgctgttgcc gtcacttttc
     1861 aggacatact cataggtccc aagatcaacg aaaccgccgg tattgcccag cgtaaacgaa
     1921 gcatcccctc cccctgtttt caccagcgtc atcgcgtcgt cagactgtgg gctgacgccg
     1981 gtatcctgaa caaagatttt aaaattacca gtggcgttgt tgttgacgac cagttgatcg
     2041 ccccgggagc ctgcaacgtt ggtatgcagg tagaaattac cgctaccgga aagttcattg
     2101 gttgtcagcg tattgtagat accgttagtt gtggcaggcg ctgctgtttg tgctgacaaa
     2161 taaacatcac cgtcgttgag tagcaaactg tttactctat attcgcagtt gctggtacct
     2221 gcacaggaat tattgctgtt aagccagaca ctcccccggc tggcagccgt caggtcggca
     2281 agcgtggtat cgacgccatt tcccagagtc aatgtcgcgc tatcggtaat ccggaccgcc
     2341 ccttcgagtt taactggcgt aacattatcc cgtggcgtca ttaacgacag gcttcctgtc
     2401 gcgccactga ccgatgcatc cgccagcgta cctgcgtaga cgattgccgt accgccagcg
     2461 acctggagat cttctgcccg ggccagagcc tgaaactcat cttttgaccg cccaagggta
     2521 tattgcccgc cagagttaac ctttgtggcg gagtcctgac ccaggttttg cattgcccca
     2581 cccttgccaa ccgtggagtc gcgagcttcg gtacctgcta ataccaataa attaccgcca
     2641 ttttccagca acatattggt cgctaaattg ccggaaatgg aaaaagtacc gtactggtga
     2701 gtaccgctga tttcaatacc gttagccgtg ctcgtctgga gagcggcacc gctgttctgg
     2761 acgatatctg tcgctttgcc attatcgttg actgtcagcg taccgccttc attgatcttt
     2821 gtttttattg cctctccgtt agctgaaact gtttgtattc cgccgtcgtt aattgttgtc
     2881 tcattcgcca caccctcgac aatttgttca ccgccggtga gcgtcgtgcc tgtcgcagtg
     2941 gcttttgttt tgacgatctc ccgtccgccc atattgacct gtgttttgtc agaagaggtg
     3001 tctgactcca cggttaacac gccgccattt gccagcagga tattgttcgc cgcaccctgc
     3061 tcgatgctga acgcgacgcc atccgcgcgt gttcctgtga cccgcgtcgc cctggtggtt
     3121 gcaaccaaag cgccctggct actctgctgt atccccgttg cgctgccttt ctcccgcaca
     3181 tcgagtgtgc cgccgtcatt aagcaccgag ttttcagcca gaccgccctc attaactacc
     3241 tgtgaacccc cattaataat ggaaccttcc gctgtcccgt ttgccataat ttgttgtagg
     3301 ccagagacga tatcggtatt gattgcctta ccataattct gaacggtttg cgtgccacca
     3361 ttgatgtgtg ttttctctgt tgacccacca tcaacaattt gttcaccact ttcgatattt
     3421 gcttcagtgg ctaaaccata taccgtttgc ttgccacctt tgatatttgc tttatcagaa
     3481 gtggcactgg catatattgt ttgggtgcca gcactattga gtacagtgcc aacatctttt
     3541 ccataaacat ccattttgcc gttggcatta ataatcgtgt caactgcgcg ggaaccagtg
     3601 acgactgtta atgagccagc gttttccagc actacatttt tagcttctga attcctgatg
     3661 tagaaagcat caccataact gttggttcct tcgataagtg ttccggaggt cgtggaagca
     3721 attaatgcgc cgccggattg ttgctcaaca tgcttagcct caccaccgtc ctgaacctcc
     3781 agaacgccgc cattattaag tctggtgcta tctgttttag cctccttctg gacaatcagc
     3841 ttaccgccag tatcaatggt agtatttttc gccgaggttt tagccactac cgtcagttcg
     3901 ccggtatttt ccagcacaac ataattagcc tcccctccgg taatagtgaa gtgagagagc
     3961 ttgttgtatc cttcgatatc agtccctgca cccgtgttgg caactaaagc actgcccgtc
     4021 tcctggttaa ccccatgtgc aataccgccg gtatagacaa tcagcgagcc tccggcgcta
     4081 atattgctgc caattgccgt accatctttc tcaacaacct gccggctccc ggaggatata
     4141 attgttgtgt cagctttccc gccgcttttg atattttgcg ttccgctgtt gatattggtg
     4201 cctgtggcta tgccataatt attaatattc tgtgtgccac cattaattat ggtatttgtc
     4261 gcgtttcctg caacatccat aaccccgcca ttatctattc gggtcgcatc agctttagca
     4321 ttggttaaaa ctgacattgt tcctttatct ttaataatcg tcttgtttgc cgaaccatat
     4381 gcgtttatgt ctaaatgacc accgttttcc agcaacacat tgtctgccac gtgattgtgg
     4441 atggagaatg caccttcact attcgtaccg ctcaccgtcg taccgttagt gttagttttt
     4501 aaaattgcac catcgtgctg ggtaacattt gttgccgtac caccactaac atcaagcacg
     4561 ccaccagaat aaacttcaat aacatccgag gtgctggtgt tatcaacaat ttgcgtgcca
     4621 ccagaataga tatgcgtatt ttttgccgtt gacttactat tcagagactg agttccgcct
     4681 tcaatcgtcg tgtccagcgc acggctctca tatactcttt gctcaccgcc atttttaatg
     4741 gttgttgttt ctactgtgct ctgttcaaca tactgtcgac caccatttat ggttgtgttc
     4801 gttgccagac ttccttgtac tacgtcctga gagccagact tgtttatcgt tgtaccatca
     4861 gcatgccctt gaacttttac tatctggcta ccaccatcaa tgagtattcc attcgcactc
     4921 cctccctcta cgcgtgaagc accgccctta attgtcgttc cattgctgat acccccttta
     4981 taaacgtcct gattgccact ctcgattgtc gtacctgtgg aaataccccc gtcatgaatt
     5041 gactgtctgc caccgttaat ggttgtatta ttagcctgcc ccacaaaatt gttatgactt
     5101 cctatatctt gatatccacc agattcaata agacttccat tagatacccc gccatgaaca
     5161 ttctgctggc catggttgat aatatgagtg ttatttgttg tacctcgttc atccactttt
     5221 tggttgccat ctacagtctc atcgtttacc acaccaataa cattagtagt gaaggcagcc
     5281 atcccgggcg ggcatatatc aaggcagata tcaataagga aagtactgag cggcgacaat
     5341 aatagggact ggtcctgttc ataaatttca tcctctgaaa agtgaatact gagtagcgtt
     5401 taagcgacct tagctttgct gcaacatcag cccacaggca ccagaccagg ggattcatcc
     5461 tgaagagaca gcgcaagtgt attgtgttca ccgctcatca aagacatcat gatgaaatga
     5521 tgatattccg cataagaatg aggcattttt taaacgcagt gcgctgaagt gtggttggat
     5581 aaaaaagtca atccattcag gaaatacggg cgtattcttt tctttcgaca atgaggccgt
     5641 tggcaaaata aaatgattta cataatcgtt tctgatgaat atcttctgct cacataaaaa
     5701 tcacacaata actttgagat cgcagattgt tttactttta cagcattcgt ccccccattg
     5761 ttgggcaaat atagattggg ccagagcacg aaagttaata ccacgttcgc acagctcctc
     5821 caacagcacg acaagatgcc acatactgcg ccccagtcgg ttcagtttac agactagcag
     5881 tgcgttccct gccgatgatg tcctgaccag ttttttcagt ccggacctcc tgccacggtt
     5941 ttacgttgaa gtggtaaccc tgagcacgca gttcttcagt caggcgtggt gcaccgtaac
     6001 gctgttattg ctgggtaaga tcaaaaaact ttcaggcagc taaggaaagt tgaaccagac
     6061 attagatgaa atatttcaac caattacagc accaattcag tcactgccag cccaccaaat
     6121 aaatcaaggg gttacatgaa aacgtagccc ctttttcttt ggtagtgaca ctaaaatgga
     6181 tgtagtgtga agaataatcc cgtttactca atcaataata catattgttt caatctacgt
     6241 tattatctct ttgtaaaaat tgccatttat taatcattga aaactgcttt tagaacttga
     6301 tacaacggga ctagtcacaa caggactatt ctcaacggga tcatcctcag aggaactatc
     6361 atcaaagtca tcatccataa ataaaatatc atcgaatggt gccacgcccg tgatgagttt
     6421 tattttatta ttacgatcag tcaatactcc acttaaaccg ttttcgctca caggttttaa
     6481 tgatttttca ttactcttgt tgtaagcagg cgcattaaaa atacacggag tatcaacatc
     6541 aaacaatgac gttccccagt tcacatattg aatatcatag ttactgaagt tctgtccaga
     6601 aaagaagcac cccttaaaat ccaatccacg taaattatat aaaccaccct cttctttttg
     6661 gagagtaatg ttaattttgg ctatctcccg gacatcatcg ccatttttgt atttgaatac
     6721 cgtttcaaga tgttctccag acagcttgac ttcaggaaat aatttgaaat caaagcctat
     6781 attattatgt gtcaacgtag atgaacaaaa aatggaaaaa gcttgcagtg ctgaattata
     6841 gctatcgatt ttatcttgag gctgagctct tggtaaaaac ttatagcaac actcataaaa
     6901 attaaacaga agctctgaag accttgtatt tttatcaaat aataccccct tgaactcatt
     6961 cacaaacgca tctttttttt cttgaatatc tatggggtgt tcctttgact ctgacaaaga
     7021 tgaaatctca tctattttgt tttcatatga attacgtgat tccatacaga catttggcgg
     7081 cgtttctaaa ataacactac gcgtactact tggcttaagt aaaccaacat gaaaatcact
     7141 ttttcttata ttatcgaaaa ggttctattc atttctttta gcgcattcaa aaaactgatc
     7201 ggcattattt ttattcgata attttttagt ttcagaaaac acattttcat tgttttccag
     7261 ctttagttta atgagaagat tttcccagac ctgcttgctt aaacatatta cgtcaggctc
     7321 accagaacta actaactcgt gattattatt aaagtgtacg ttgaatacct ttaagttatt
     7381 ttcaccaact tcatatttaa tacgttttaa ttgttctcca gctcccataa tgacaaaggc
     7441 gttgccttct tgatatatac attcagacat cattttttgt aaagtttcaa gagcaccgcg
     7501 atacgtctct gatgcagctt ccttacaaat taattttaaa atactatgag ctagtagctc
     7561 catgtgttta gaagatttat tttcattata acttccacta cccaaaactg cgctagcgtt
     7621 aaatccgttg cttttactaa ctaacattgt ctattcctca attaatgtct acatggctat
     7681 ttttaatgtt attactgttt gtcactataa aaaaacgctc atttgagaca attactgaca
     7741 ttaactgctt cacttgctac gcatggaact tttaattaaa ttagcacagg aatgttaaat
     7801 ttaataaaca aaaggttatt tcgctgtatg ataaaaaaaa ccgttataat ttattagtga
     7861 aaatcgtttt tcaagtgtta gaaatttata tctcaatagc gttggttaat gagcatagcc
     7921 acgctcctgt aacgctcaca aaactcatct gcctgcggcg ggtgttctgg tcagtagtag
     7981 atgtttaagg cgtggcagag acatttcatc cttactctac ggcattgttc tacatacatt
     8041 ggttgtggta ctcacttatc atcagtgagc gaacagagaa tagttcagtg atttgagtaa
     8101 ttaacctgat taaatgaagg ggtataataa atgataatac tctggcttta tcgttaatta
     8161 cttaattcca catgtaagca atttgcccgc ttggcatagc aggcattttt tccaggtact
     8221 tttgaatgag tactgatgga taaatacatt gcagtggcgt gccacgtacc aaaacaccag
     8281 ccctcattcg aaaccaccca ccgcacttct tccttgaaat ggcgttagtc atgaaatata
     8341 gaccgccatc gagtacccct tgtaccctta actcttcctg atacgtaaat aatgatttgg
     8401 tggcccttgc tggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact
     8461 gagctaaagg gccttgagtg tgcaataaca atacttataa accacgcaat aaacatgatg
     8521 atcatatgat gtaaataaca gatttttatg cgttcccatt caccttgggt cgtgattaca
     8581 cgcgacataa aacccgacac cgcctccatt cgcaaagtcg atactcgcag tcaacaagca
     8641 aatgttaata attagcacta tctatagtta tcatcgattc aatgatactt tgtaatgatt
     8701 ttgtatctaa taatataact ttattacatt agctgaagag ttttcgcatc attatgatat
     8761 ctgttacttt tcactccata aaaat
//
LOCUS       DBBGLQ010000149         8781 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.149, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000149 DBBGLQ010000000
VERSION     DBBGLQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.149"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..1167
                     /gene="umuC"
                     /locus_tag="ACIYSB_004771"
     CDS             <1..1167
                     /gene="umuC"
                     /locus_tag="ACIYSB_004771"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGC5431641.1"
                     /translation="GCVIARNAEAKALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYA
                     DMSNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVG
                     VGIAQTKTLAKLANHTAKKWQRQTGGVVDLSNLERQRKLMSALPVDDVWGIGRRISKK
                     LDAMGIKTVLDLADTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRS
                     FGERITDYPSMRQAICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASV
                     KLLTPTQDSRDIINAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRP
                     GSEQLMTVMDTLNAKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1213..1743)
                     /gene="dsbB"
                     /locus_tag="ACIYSB_004772"
     CDS             complement(1213..1743)
                     /gene="dsbB"
                     /locus_tag="ACIYSB_004772"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGC5431642.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(1889..3430)
                     /gene="nhaB"
                     /locus_tag="ACIYSB_004773"
     CDS             complement(1889..3430)
                     /gene="nhaB"
                     /locus_tag="ACIYSB_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGC5431643.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3651..4370
                     /gene="fadR"
                     /locus_tag="ACIYSB_004774"
     CDS             3651..4370
                     /gene="fadR"
                     /locus_tag="ACIYSB_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGC5431644.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4422..5954)
                     /locus_tag="ACIYSB_004775"
     CDS             complement(4422..5954)
                     /locus_tag="ACIYSB_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGC5431645.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            6284..7582
                     /gene="dadA"
                     /locus_tag="ACIYSB_004776"
     CDS             6284..7582
                     /gene="dadA"
                     /locus_tag="ACIYSB_004776"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGC5431646.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWIFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7592..8662
                     /gene="dadX"
                     /locus_tag="ACIYSB_004777"
     CDS             7592..8662
                     /gene="dadX"
                     /locus_tag="ACIYSB_004777"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGC5431647.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIE
                     RIWSAIGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
BASE COUNT         2182 a         2192 c         2385 g         2022 t
ORIGIN      
        1 ggttgcgtta tcgcccgaaa cgctgaggca aaggcgcttg gcgttaaaat gggcgatccc
       61 tggttcaaac aaaaagatct gtttcgtcgc tgtggcgtgg tttgctttag cagcaattat
      121 gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagagct atcgccccgc
      181 gtcgagattt acagtattga tgaggcattc tgcgatctga caggtgtgcg taattgtcgc
      241 gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac ccatcttact
      301 gttggtgtgg ggatcgccca gaccaaaacg ctggctaagc ttgccaatca tacggcaaaa
      361 aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg ccagcgtaaa
      421 ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat cagcaaaaaa
      481 ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat ccggtttatc
      541 cgtaaacatt ttaatgtcgt gctcgaaaga acggtgcgtg aactgcgcgg cgaaccctgt
      601 ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg ctcgtttggt
      661 gaacgcatca cggattatcc gtcgatgcgg caggccattt gtagttacgc tgcccgggcg
      721 gcggaaaaac ttcgcagcga gcatcaatat tgtcggttta tctccacgtt tattaagacg
      781 tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa actgctgacg
      841 cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga tgccatctgg
      901 caagcgggcc atcgttacca aaaagcgggc gtgatgctgg gggatttctt cagtcaggga
      961 gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga gcaattgatg
     1021 acggtaatgg atacactgaa tgctaaagag ggcagaggaa cactctattt tgccgggcag
     1081 gggatccagc aacaatggca gatgaagcga gccatgcttt caccacgtta tacaacgcga
     1141 agttctgatt tactgagggt caaataaata tagcggcagg aaaaaagcgc tcccgcagga
     1201 gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg gctgggaaat
     1261 caccaccagc actgcgacaa tcaggtaagc gataaaaata ccgagcagcc actgcggcat
     1321 ttccagacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga caaacacttg
     1381 cggcacccac ttatccagcg gcagccattc cgggaaacga accataaaat cacaggtggc
     1441 aaacggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca caccgcggaa
     1501 cgcactatac aaccagataa ccatcgctac ataacgcagt ggcgtttttg gggcgatcgc
     1561 gccaatcagc gcagcaccca gaacgccgaa taatgcgcag cgttcataaa tacagagcac
     1621 gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca gtgccagagc
     1681 agtaaacgcc atcaacaacc acgcgccccg gccttgtgaa cattggttca aaaatcgcaa
     1741 cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc attcgctgcg
     1801 ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa aaggcaaacc
     1861 gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc atttgcataa
     1921 accattcggt tacaggggca agcgtaaact cgacgcagag cagtccgacg agtgtcagga
     1981 cgagggtgta aggcaaggcc atccacacca tgcggccata agagaggcga atcaatggcg
     2041 cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta gcgacggagg
     2101 gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc aacgtgatag
     2161 cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg aagacgttat
     2221 ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga gcatgttccg
     2281 atgcctgcaa cacaaactga ataattggcg aaaacagttg ttggtcgata atcaccgcga
     2341 ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg aaggctttac
     2401 cgatagcatg ctcatcggtg acaccggtca atgatgttgc cagaataatg actgacaaac
     2461 caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag acgccaataa
     2521 tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg ctttgatcgt
     2581 caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag ccaaaccaac
     2641 gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga acggtcaccg
     2701 gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg atgatcaggt
     2761 tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca ccggcatgca
     2821 tcatcaggct acgcagaaag ccacggaact gttccagaac cactttgtaa tgcttgtcga
     2881 tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg cggtgataaa
     2941 taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag gcatcgagga
     3001 acgcggagag gaacgcagcc gccacgcaaa aagagagcga cagcagcatt ttggagcgaa
     3061 tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa tagatacccg
     3121 ccaccataaa catcagtaac agcaagactt caagatttgc cgccacctct tcacggacgt
     3181 gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca ccggggagca
     3241 gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc agcaaccagc
     3301 ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa atgatgaggg
     3361 cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg cggccccagg
     3421 agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt atgtttaaac
     3481 gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg gaaagagatg
     3541 cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg tagttagccc
     3601 tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact atggtcatta
     3661 aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc tggaataacc
     3721 gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta attggcgtaa
     3781 cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg ttgaccattc
     3841 aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta aatatccttg
     3901 aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat ttgctgtcgg
     3961 tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat cccgataaag
     4021 cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc tttgccgagc
     4081 tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt tacggtctga
     4141 ttcttaacgg gatgaaaggg ctgtatacgc gtattggtcg tcactatttc gccaatccgg
     4201 aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc agtgaaggcg
     4261 cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc gagatttggc
     4321 accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa tcccttccgt
     4381 ttaaagagca aacccctcaa acgaggggtt ttttgttgtt tttacagatt tcccattctt
     4441 ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc gagcatcaca
     4501 tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg atccagcggt
     4561 gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa atccacgttc
     4621 cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa ccggttacgg
     4681 atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt atgccgatcg
     4741 tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag gaactggctg
     4801 ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag ccagtcggaa
     4861 ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca aatccgttta
     4921 atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta ccacgggctg
     4981 ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag cataaaacgt
     5041 tccgttactt tcccttcatc atacagatgg ttaaggatgg tgtagtgcca gaaggtcgcc
     5101 cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct caccttacgc
     5161 acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt ctccataaaa
     5221 tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac cgttttctct
     5281 tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgcag atactcttcg
     5341 cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg tttgtaccgg
     5401 tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac ttcatcaacg
     5461 ccataacgct cttcgcactc ggtaatatat ttacgggcaa aaatcagata atcgacaatc
     5521 gaactggcgt cggtccagct acggaataag taattgtttt tgaagaaaga gttatgccca
     5581 tagcaggcat gagccatcac cagcgcttgc atggtaatgg tgttctcttc catcaggtaa
     5641 gcgatacacg ggttagagtt aatgacgatt tcataggcca gtccttgctg accgtgctta
     5701 tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata gttaattggc
     5761 atgccgacgc tggagtaggc atccatcatc tgttctgagg ttatcacttc aatctggtgc
     5821 gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag ataaacatcc
     5881 agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt attcatagaa
     5941 tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg agcacctcat
     6001 ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc tttgcgattt
     6061 cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg ataaaaaggg
     6121 cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga tgtgagccag
     6181 ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca agatgtgatt
     6241 agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag ttgtcatact
     6301 gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag gacatgaggt
     6361 caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga atgccgggca
     6421 aatctccccc ggatatgctg caccgtgggc ggcaccaggt gtgcctttaa aagcgattaa
     6481 atggatattc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc agttccagtt
     6541 gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg aaaacaaagg
     6601 gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc gcgccgaaac
     6661 caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta ccgaacaaca
     6721 gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac cgtatcagct
     6781 gctggaatcc agccgcctgg cggaagtgga gcccgcgctg gcagaagtgg cgcacaaact
     6841 gacgggcggc ctgcagttac ccaatgatga aaccggagac tgtcagctat ttacccagaa
     6901 tctggcgcgg atggcggagc aggcgggggt taaattccgc tttaatacgc ccgttgacca
     6961 actgctttgc gacggcgagc aaatctacgg cgtgaagtgt ggcgatgaag tgattaaggc
     7021 cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag gcattgttga
     7081 tattccggtg tatccgctga aaggctactc gctgaccatt ccaattgcgc aggaagatgg
     7141 tgcgccggta tccaccattc ttgatgaaac ctacaaaatc gccattaccc gtttcgataa
     7201 ccgcattcgt gttggcggaa tggcggagat tgttggtttt aataccgagc tgttgcaacc
     7261 gcgtcgtgaa acgctggaga tggtggttcg cgatctctat ccacgcggcg gtcatgtcga
     7321 gcaggcgact ttctggactg gtctgcgccc gatgacgcca gacggcacgc cggttgtcgg
     7381 gcgtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg gctggacgat
     7441 ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc cagcgatccc
     7501 atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac gtccgggcca
     7561 tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg ccagcctcga
     7621 tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgacgc acgcgcgcgt
     7681 ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct ggagcgcgat
     7741 cggggccacc gatggctttg cattgcttaa cctggaagag gcaataacgt tacgtgagcg
     7801 cggctggaaa ggaccgatcc tgatgctgga aggatttttc catgctcagg atctggagat
     7861 ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca aagcactgca
     7921 aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg ggatgaatcg
     7981 gttgggcttc cagcccgatc gcgtgcttac cgtctggcag cagttgcggg caatggcgaa
     8041 tgttggcgaa atgaccctga tgtcgcattt tgccgaagcg gaacatcctg atggaatttc
     8101 cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc gttcgttgtc
     8161 caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc ggcctggcat
     8221 tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata ccggattacg
     8281 tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag cgggcgagcg
     8341 tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca ttgtcgccgc
     8401 agggtacgcc gacggttatc cgcgccacgc gcctaccggt acccctgttt tagtggacgg
     8461 cgtgcgcacc atgacggtgg ggacggtctc gatggatatg ctagcggtcg atttaacgcc
     8521 ttgcccgcag gcgggtattg gtacgccggt tgagctgtgg ggcaaggaga tcaaaattga
     8581 tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc tggcgctacg
     8641 cgtcccggtt gtgacggtgt aacttgttgt aagccggatc ggaggcaacg tcttctgggt
     8701 gcaaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga cagagccatt
     8761 gcccatgata gtgtccatta a
//
LOCUS       DBBGLQ010000150         8723 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.150, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000150 DBBGLQ010000000
VERSION     DBBGLQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.150"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(28..96)
                     /gene="ykiE"
                     /locus_tag="ACIYSB_004778"
     CDS             complement(28..96)
                     /gene="ykiE"
                     /locus_tag="ACIYSB_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HGC5431648.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            233..754
                     /gene="fecI"
                     /locus_tag="ACIYSB_004779"
     CDS             233..754
                     /gene="fecI"
                     /locus_tag="ACIYSB_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HGC5431649.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            751..1704
                     /gene="fecR"
                     /locus_tag="ACIYSB_004780"
     CDS             751..1704
                     /gene="fecR"
                     /locus_tag="ACIYSB_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HGC5431650.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1791..4115
                     /gene="fecA"
                     /locus_tag="ACIYSB_004781"
     CDS             1791..4115
                     /gene="fecA"
                     /locus_tag="ACIYSB_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HGC5431651.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4160..5062
                     /gene="fecB"
                     /locus_tag="ACIYSB_004782"
     CDS             4160..5062
                     /gene="fecB"
                     /locus_tag="ACIYSB_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HGC5431652.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5059..6057
                     /gene="fecC"
                     /locus_tag="ACIYSB_004783"
     CDS             5059..6057
                     /gene="fecC"
                     /locus_tag="ACIYSB_004783"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HGC5431653.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLMPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6054..7010
                     /gene="fecD"
                     /locus_tag="ACIYSB_004784"
     CDS             6054..7010
                     /gene="fecD"
                     /locus_tag="ACIYSB_004784"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HGC5431654.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7011..7778
                     /gene="fecE"
                     /locus_tag="ACIYSB_004785"
     CDS             7011..7778
                     /gene="fecE"
                     /locus_tag="ACIYSB_004785"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HGC5431655.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8336..8593)
                     /locus_tag="ACIYSB_004786"
     CDS             complement(8336..8593)
                     /locus_tag="ACIYSB_004786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431656.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
BASE COUNT         1898 a         2489 c         2426 g         1910 t
ORIGIN      
        1 tccccatcct ttagggcagt gaggatgtca ataatagcag accacatcat gagtcattct
       61 gaccgacaca tgccagaagc agaggctcca tcccatttta tacctacctt ataacactta
      121 gaaaaacaac atgttaaaaa tgtctattgg aaacaatttt atttccaatt gtaatgataa
      181 ccattctcat attaatatga ctacgtgata attaactttt gatgcactcc gcatgtctga
      241 ccgcgccact accacagctt ccttaacgtt cgagtcgctt tatggcacac atcacggctg
      301 gttgaaaagc tggctgacgc gcaaactcca gtctgctttt gatgcagatg acattgccca
      361 ggacactttt ttgcgggtaa tggtcagcga aacgctctcg acgatccgcg atcctcgctc
      421 cttcctctgc actatcgcca aacgcgtgat ggtggacctg tttcgccgaa acgcgctgga
      481 aaaagcgtat ctggagatgc tggcgcttat gccggagggg ggagcgcctt cacctgagga
      541 acgcgaaagc caactcgaga ccctacaact cctcgacagc atgctggacg ggctaaacgg
      601 caaaacacgt gaagcgtttc tgctttcgca actggatggt ctgacataca gcgagattgc
      661 gcacaaactc ggtgtttcca tcagctccgt gaaaaaatac gtggcgaaag ccgtcgagca
      721 ctgcctgctg ttccgtctgg agtatgggtt atgaatcctt tgttaaccga ttcccgccgt
      781 caggcgctgc gttcagcttc ccactggtat gccgtgctaa gcggcgagcg cgtcagccca
      841 caacaggaag cgcgctggca acagtggtat gaacaggatc aggataacca gtgggcctgg
      901 cagcaggttg aaaacctgcg caaccagctt ggcggtgtgc ctggcgacgt tgccagcagg
      961 gcgttgcacg atacccgcct cacccgccgt cacgtgatga aaggattact gctgttgctc
     1021 ggcgctggcg gaggctggca gctctggcag tcggaaaccg gcgaaggtct gcgggcagat
     1081 taccgcaccg ccaaaggcac agtcagccgt cagcaactgg aagatggctc cctgctcacg
     1141 ctgaataccc aaagcgcggc ggatgtgcgt tttgatgcgc atcagcgcac cgtccggctc
     1201 tggtacggtg aaatcgccat tactaccgcg aaagatgccc tgcaacgccc cttccgtgtc
     1261 ctgacccgtc agggccagct cactgcttta gggacagaat ttaccgtccg ccagcaggat
     1321 aatttcacgc agcttgacgt gcagcagcac gctgtggaag tgcttctcgc cagtgccccc
     1381 gcgcaaaaac gcatcgtgaa cgctggtgaa agcctgcagt tcagcgcctc tgagtttggc
     1441 gcagtgaaac cgctggatga cgagagtaca agctggacga aggacatcct gagcttcagc
     1501 gataaaccgc tgggtgaggt gatagccacg ctaacccgtt accgcaacgg cgtgctgcgc
     1561 tgcgatcccg ccgttgccgg gctgcgcctg agcgggacgt tcccgctgaa aaataccgat
     1621 gcgatcctga acgttatcgc gcaaacgctt cccgttaaaa ttcagtctat tacgcggtac
     1681 tggataaaca tttcaccact gtaaggaaaa taattcttat ttcgattgtc ctttttaccc
     1741 ttctcgttcg actcatagct gaacacaaca aaaatgatga tggggaaggt atgacgccgt
     1801 tacgcgtttt tcgtaaaaca acacctttgg ttaacaccat tcgcctgagc ctgctgccgc
     1861 tggccggtct ctcgttttcc gcttttgctg cacaggttaa tatcgcaccg ggatcgctcg
     1921 ataaagcgct caatcagtat gccgcacaca gcggatttac cctctcggtt gacgccagcc
     1981 tgacgcgcgg caagcagagc aacggcctgc acggcgatta cgacgtcgag agcggcctgc
     2041 aacaactgct ggacggcagc ggactgcagg taaaaccgct aggaaataac agctggacgc
     2101 tggagcccgc gcccgcacca aaagaagatg ccctgaccgt ggtcggcgac tggctgggtg
     2161 atgcgcgtga aaacgacgta tttgaacatg ctggcgcgcg tgacgtgatc cgccgtgagg
     2221 atttcgccaa aaccggcgca accaccatgc gtgaggtact taaccgcatc cctggcgtca
     2281 gcgcgccgga aaacaacggc accggcagcc acgacctggc gatgaacttt ggcatccggg
     2341 gcctgagccc gcgcctcgcc agccgctcga ccgtcctgat ggacggcatc cccgtcccct
     2401 tcgcccctta cggtcagccg cagctttcac tggctcccgt ttcgctcggc aacatggatg
     2461 ccattgacgt ggtacgcggt ggtggtgcgg tgcgttacgg accgcagagc gtgggcggcg
     2521 tggtgaactt tgttacccgt gccattccgc aggactttgg tatcgaggcg ggcgtggaag
     2581 gtcagctcag cccaacctct tcacaaaaca acccgaaaga gacgcacaac ctgatggtgg
     2641 gcggcacagc ggacaacggt tttggcaccg cgctgctcta ctccggcacg cgcggcagtg
     2701 actggcgcga gcacagcgcc acccgcatcg acgacctgat gctgaaaagc aaatatgcgc
     2761 cggatgaggt gcacaccttc aacagcctgc tgcaatatta cgatggtgaa gccgacatgc
     2821 ccggtggcct gtctcgcgcg gattacgacg ccgatcgctg gcaatccacc cgcccgtatg
     2881 accgcttctg gggtcgtcgc aagctggcga gcctgggcta ccagttccag ccagacagcc
     2941 agcataaatt caacattcag gggttctaca cccaaaccct gcgcagcggc tacctggagc
     3001 aaggcaaacg catcaccctc tcgccgcgta actactgggt gcgcggtatt gagccacgct
     3061 acagccagat ctttatgatc ggcccttccg cgcacgaagt gggcgtgggc tatcgctatt
     3121 tgaatgaatc aacgcatgaa atgcgttact acaccgccac cagcagcggg cagttgccgt
     3181 ccggctcaag cccttacgac cgcgatacgc gttccggcac cgaggcgcac gcctggtatc
     3241 tggatgacaa aatcgacatc ggcaactgga ccatcacgcc gggtatgcgt ttcgaacata
     3301 tcgagtcata ccagaacaac gccatcacag gcacgcacga agaagtgagc tataacgcac
     3361 cgcttccggc gttgaacgtg ctctatcacc tgactgacag ctggaatctt tatgcaaaca
     3421 ctgaaggctc gttcggcacc gtacagtaca gccagattgg caaggctgtg caaagcggca
     3481 atgtggaacc ggaaaaagcg cgaacctggg aactcggtac ccgctacgac gacggcgcgc
     3541 tgacggcgga aatggggctg ttcctgatta actttaacaa tcagtacgac tccaaccaga
     3601 ccaacgacac cgtcactgca cgtggcaaaa cgcgccatac cgggctggaa acgcaggcac
     3661 gttacgatct gggtacgcta acgccaacgc ttgataacgt ttccatctac gccagctatg
     3721 cgtatgtgaa cgcggaaatc cgcgagaaag gcgacaccta cggcaatctg gtaccattct
     3781 ccccgaaaca taaaggcacg ctgggcgtgg actacaagcc aggaaactgg acgttcaatc
     3841 tgaacagcga tttccagtcc agccagtttg cggataacgc caatacggtg aaagagagcg
     3901 ccgacggcag taccggccgc attcccggct tcatgctctg gggcgcacgc gtggcgtatg
     3961 actttggccc gcagatggca gatctgaacc tggcattcgg tgtgaaaaac atcttcgacc
     4021 aggactactt catccgctct tatgacgaca acaacaaagg catctatgca ggccagccgc
     4081 gcacgctgta tatgcagggg tcgttgaagt tctgatgtgt taacgcccgg cttgccgggc
     4141 ttttagctgg aatgtgatta tgttggcatt tatccgtttt ctttttgcag gcctgctgct
     4201 ggtgatcagc cacgcctttg ccgccatggt tcaggacgaa cacggcacgt ttacactcga
     4261 aaaaacgcca caacggattg tggtgctgga actctcgttc gccgatgcgc tggccgccgt
     4321 ggacgtcagc ccgatcggta ttgccgacga taacgatgca aaacgcatcc tgcccgaagt
     4381 gcgtgcgcac ctgaaaccgt ggcagtccgt cggaacgcgc gcgcagccga gcctggaagc
     4441 cattgccgct ctgaaaccag acctgatcat tgccgacagc agtcgccatg cgggggttta
     4501 catcgccttg cagcaaatcg cgccggtact gctgcttaag tcccgcaacg aaacctacgc
     4561 tgaaaatttg caatctgcgg ctatcatcgg cgaaatggtg ggtaaaaagc gagagatgca
     4621 ggcacgtctg gaacaacata aagagaggat ggcgcagtgg gccagccagc ttcccaaagg
     4681 gacacgcgtg gcctttggca catcacgcga acagcaattc aacctgcata ctcaggagac
     4741 ctggaccggc agtgtgctgg cctctctggg gctgaacgtt cccgctgcga tggcgggtgc
     4801 gtccatgccg tccatcggcc tggagcaact gctggcggtc aatcctgcct ggctgctggt
     4861 tgcccactat cgcgaagaga gcattgttaa acgctggcaa caagatccgc tctggcagat
     4921 gttaaccgcc gcgcagaagc agcaggttgc ttcggtcgac agtaacacct gggcgcggat
     4981 gcgcggtatt tttgctgcag agcgtattgc cgctgacacg gtaaaaatct tccaccatca
     5041 gccgcttacc gttgtgaaat gaccgcgata aaacacccgg tgctgctgtg ggggcttccc
     5101 gttgcagcac ttattattat tttctggctg agtctgtttt gctactcggc cattcctgtt
     5161 tccggagcag atgcaacccg cgccctgctg cctggacaca cgccaacgct accagaagcg
     5221 ctggtgcaaa accttcgttt gccacgaagc ctggtcgccg ttctgatcgg cgcaagcctg
     5281 gcgctcgcgg gcacgctgct gcaaaccctg acccacaacc caatggcctc tccttcactg
     5341 ctcggcatta acagcggcgc ggcactggct atggcgctta ccagcgcgct gagtccgacg
     5401 ccgattgcag gctattctct gtcgttcatc gcggcatgcg ggggcggcgt gagctggctg
     5461 ctggtcatga ccgcaggagg cggatttcgt catacccatg acagaaacaa actgatcctc
     5521 gcgggtatcg cgctgtcggc cttttgtatg ggccttaccc gcatcaccct gctgctggcc
     5581 gaagatcatg cttacggcat cttttactgg ctggcaggcg gagtgtccca cgcccgctgg
     5641 caggatgtct ggcagctctt gccggtggtg gtcactgcag tccctgtcgt gttgctgctg
     5701 gcgaatcaac tgaacctgct caacctcagc gacagcaccg cccatacgct gggagtgaac
     5761 ctgacgaggc tacgtttggt catcaatatg ttagtgctgc ttctggttgg tgcgtgcgtc
     5821 agcgtggcag gtccggtggc gtttatcggt ctgctgatgc cacatctggc gcgcttctgg
     5881 gcaggcttcg atcagcgcaa cgtactgcca gtgagcatgc tgctgggggc cacgctgatg
     5941 ctgctggcag atgtactcgc acgcgcgctg gccttccccg gagatctgcc cgcaggcgca
     6001 gtgctggcgc tgattggcag cccttgcttt gtctggcttg tgaggaggcg aggatgaaaa
     6061 ttgcgctggt tattttcatc acccttgccc tggcgggctg tgcgctgtta tcactccata
     6121 tgggagtgat ccccgtgccg tggcgcgcgc tgctgaccga ctggcaggcc ggacgcgagc
     6181 attattatgt attgatggag taccgactgc cgcgcttgct gctggcactg tttgtcggtg
     6241 cagccctcgc cgtggcgggc gtgctgatac aggggattgt gcgcaaccct ctggcatcac
     6301 cggatattct cggcgttaac catgccgcca gcctggcctc tgtgggggct ctacttctta
     6361 tgccgtcact gcccgtgatg gtgctgccgc tgctggcctt tgcgggcggc atggcggggt
     6421 tgatattact gaagatgctg gcaaagaccc accagccgat gaagctggcg ctcaccggcg
     6481 tggcgctttc tgcatgctgg gccagcctga cggattatct gatgctctcg cgcccacagg
     6541 atgtgaacaa cgccctgctg tggctgaccg gcagcttatg gggccgtgac tggagctttg
     6601 tgaagattgc catcccgctg atgattttat ttctgccgct gagcctgagt ttttgccgcg
     6661 atctcgacct ccttgcactc ggcgatgcgc gcgccaccac gctcggtgtg tcggtgcccc
     6721 atacccgatt ctgggctttg ttactagctg tcgccatgac atctaccggc gtggccgcct
     6781 gcggcccgat tagctttatt ggtctcgtgg tgccgcatat gatgcgtagc atcaccggtg
     6841 gacgtcaccg cagactgctg cctgtttcgg ccctgacagg tgcgttgctg ttggtggttg
     6901 ccgatctgct ggcgagaatt attcatcccc cactggagct cccggttggc gtgctgaccg
     6961 ccattatcgg tgcgccgtgg tttgtctggt tgcttgtgag aatgcgataa atgactttac
     7021 gaactgaaaa tctgacggtc agttacggga cagacaaggt acttaacgac gtttcactct
     7081 cactgccaac ggggaagatc accgccctga tcggtcctaa cggttgcggg aaatcgacgc
     7141 tgttaaactg tttttcgcgg cttttaatgc cgcagtctgg caccgtattt ctcggcgata
     7201 atcccataaa tatgctctca tcacgccagt tggcccgcag gctttcgctg ctgcctcagc
     7261 accatttaac gccagagggg atcacagtcc aggagctggt ttcgtatggt cgtaatccct
     7321 ggctgtcact ctgggggcgt ctctccgctg aagacaatgc acgagttaat gtcgccatga
     7381 accagacccg gatcaatcat cttgccgttc gtcggttaac cgagctttcc ggcggtcagc
     7441 gccagcgcgc atttctggcg atggtcctgg cccagaatac gcccgttgta ttacttgatg
     7501 agccaaccac ctatcttgat atcaatcacc aggtggacct gatgcggttg atgggcgaac
     7561 tccggactca ggggaaaacg gtggtcgctg tgctgcacga ccttaatcag gctagccggt
     7621 actgcgatca actggtggta atggcaaacg gacatgttat ggcgcaaggc acaccagaag
     7681 aggtgatgac cccaggattg ctgagaacag tattcagcgt ggaagcggaa atacaccccg
     7741 agccggtatc tggcaggccg atgtgcctaa tgaggtagat tgcacaggcc gtaagaacca
     7801 aaccacgact gaatgaaact ggactggcgc cagcaagcct gttcagactg gggctgaact
     7861 tttccggact ctgaaagatt accaatactc atcgtccatc cgcttgcttt aggctgacag
     7921 gttcataatc aacgcaaacc agagctgtac aggcttgggc gcggctttca aaccagtcgt
     7981 gatcacggca atcaattttg aactctgctt aacggacatt tctgtataac ccttacggca
     8041 acgaaaaacg cgaagttaaa attttagaaa cccaaaaacg tgacatgact aagtttagat
     8101 ttcagggggg gagatcaaaa aatttcgctc tgtgccagag cggacattca cggagctggt
     8161 tcattaccaa tgaggttggg cttttgagga taaatcaatg atcagacgcc aacgtaaatc
     8221 aaaagcaacc ctggaacgga attgctaatc cagtttctga ccatcgattt ttctaaaaag
     8281 tgtgcgtttg ctactactta ggtaggtgca gctttcttaa tcaccggcag ccacgttata
     8341 caggccagtt gatggatcga ttgttatcaa tgatatcttc atgagtcggt gtctcaccca
     8401 gcttaccgaa gctggcataa agtgaaggca gacgggcccg tccttctccc tttttcgcca
     8461 gagggaaagc gcgaagcatg gtggcagcct cccagtcaca acaataggat ggtgtgcacg
     8521 gctgctgacg ccatgattca gcgatagagc cggaaaatac ggggtcaaag ccggtatcat
     8581 taaccagagt catcgtgacc tgtactgcgg ctggatgatc tcctgctact gcaactgcta
     8641 ggcgaccgcg gctcccttca ggtagtctgt tgtggtcaac taaaactggc ctccgcgtta
     8701 gagtttttcc agtatcggtt ttc
//
LOCUS       DBBGLQ010000151         8632 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.151, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000151 DBBGLQ010000000
VERSION     DBBGLQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.151"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            108..2738
                     /gene="traC"
                     /locus_tag="ACIYSB_004787"
     CDS             108..2738
                     /gene="traC"
                     /locus_tag="ACIYSB_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HGC5431657.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            2735..3121
                     /gene="trbI"
                     /locus_tag="ACIYSB_004788"
     CDS             2735..3121
                     /gene="trbI"
                     /locus_tag="ACIYSB_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HGC5431658.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEVQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            3118..3750
                     /gene="traW"
                     /locus_tag="ACIYSB_004789"
     CDS             3118..3750
                     /gene="traW"
                     /locus_tag="ACIYSB_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HGC5431659.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            3747..4739
                     /gene="traU"
                     /locus_tag="ACIYSB_004790"
     CDS             3747..4739
                     /gene="traU"
                     /locus_tag="ACIYSB_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HGC5431660.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            4748..5386
                     /gene="trbC"
                     /locus_tag="ACIYSB_004791"
     CDS             4748..5386
                     /gene="trbC"
                     /locus_tag="ACIYSB_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HGC5431661.1"
                     /translation="MKLSMKFLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSISE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            5383..7191
                     /gene="traN"
                     /locus_tag="ACIYSB_004792"
     CDS             5383..7191
                     /gene="traN"
                     /locus_tag="ACIYSB_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HGC5431662.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            7218..7475
                     /locus_tag="ACIYSB_004793"
     CDS             7218..7475
                     /locus_tag="ACIYSB_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HGC5431663.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEIL
                     AAAFVIFYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            7468..8211
                     /gene="traF"
                     /locus_tag="ACIYSB_004794"
     CDS             7468..8211
                     /gene="traF"
                     /locus_tag="ACIYSB_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HGC5431664.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQVQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            8227..8574
                     /gene="trbA"
                     /locus_tag="ACIYSB_004795"
     CDS             8227..8574
                     /gene="trbA"
                     /locus_tag="ACIYSB_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HGC5431665.1"
                     /translation="MSEDYLKLFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDGFRRIFICIMLSSVWS
                     GVMWFFIRGLMTG"
BASE COUNT         2114 a         2132 c         2449 g         1937 t
ORIGIN      
        1 agcctccatc tcttatttgt gctgtggttt caggaatggc actgcgtgat atatccacgg
       61 ccacagactg atattttcct tttttaaacg cactaaggaa tactcatatg agtaataacc
      121 cacttgaggc cgtcactcag gcggttaact ccctcgtcac agcactgaaa ctgcctgacg
      181 aatcagcaaa ggccaatgaa gtgctgggcg aaatgagctt cccgcagttc agccgtctgc
      241 tgccgtatcg tgattacaac caggaatccg gtctgttcat gaatgacacc acgatgggct
      301 ttatgctgga agccattccc atcaatgggg cgaatgagtc cattgtggag gccctcgatc
      361 acatgctgcg caccaaactg ccgcgcggta ttccgttgtg tatccatctg atgtccagtc
      421 agctggttgg tgacaggatt gaatacgggc tgcgtgagtt ctcctggtca ggtgaacagg
      481 ctgaacggtt taacgccatt acccgggcct attatatgaa agcggcagcg acacagtttc
      541 cgctgccgga ggggatgaat ctgcccctga ccctgcgcca ttaccgggtg tttatctcgt
      601 actgttctcc ttcgaagaaa aaaagccggg ccgacattct ggaaatggaa aacctggtga
      661 aaatcatccg ggcgtcgtta cagggggcca gtatcaccac acagacggtg gatgcacagg
      721 cctttatcga tattgtcggg gagatgatta accataaccc ggactccctg tacccgaaaa
      781 gacgtcagct ggacccgtat tctgatctga attatcagtg tgtggaggac agttttgacc
      841 tgaaagtccg ggctgattac ctgacgctgg gcctgcgtga gaacggcagg aacagcacgg
      901 cccgcatcct gaatttccat ctggcccgta acccggaaat cgccttcctg tggaacatgg
      961 ccgacaacta cagcaacctg ctgaacccgg aactgtccat ctcctgtccg ttcatcctga
     1021 cgctgactct ggtggtggaa gaccaggtga aaacccacag cgaagccaac ctgaagtaca
     1081 tggacctgga gaaaaagtcg aagacctcct atgccaaatg gtttccgtcc gtggagaaag
     1141 aggcgaagga gtggggggaa ctgcgtcagc ggctgggctc cggtcagtcc tctgtcgtgt
     1201 cctacttcct caacatcaca gccttctgca aggacaataa tgaaacggca ctggaagtgg
     1261 agcaggacat cctgaacagc ttccgtaaaa acggttttga gctgatttca ccgcgcttta
     1321 accacatgcg caatttcctg acctgtctgc cctttatggc cgggaaaggg ttgtttaaac
     1381 agctgaaaga ggccggagtg gtacagcgcg cagagagctt taatgtggcc aacctgatgc
     1441 cgttagtggc ggataacccc ctgacaccgg caggtctgct ggcacccacc taccgtaacc
     1501 agctggcgtt tatcgatatt ttcttccggg ggatgaataa caccaactat aacatggcgg
     1561 tctgtggcac ctccggggcc ggtaaaaccg ggctgataca gccacttatc cgcagcgtgc
     1621 tggactccgg gggctttgcc gtggtgttcg acatggggga tggctacaag tccctgtgtg
     1681 agaacatggg gggcgtatat ctggacggtg aaaccctgcg ctttaacccg tttgcgaaca
     1741 tcaccgatat tgaccagtca gcggagcgtg tccgcgacca gctgtcggtg atggccagcc
     1801 ccaacggtaa cctggatgaa gtgcatgaag gtctgctgct gcaggcagtc agggcatcat
     1861 ggctggccaa agagaacagg gcacgtattg atgacgtggt ggatttcctg aaaaacgcca
     1921 gtgacagcga gcagtatgcc gaatcaccga ccatccgcag ccgtctggac gaaatgattg
     1981 tgctgcttga ccagtacact gctaacggca cttatggcca gtatttcaac tccgatgagc
     2041 cgtccctgcg ggatgatgcg aaaatggtgg tgctggagct gggcggactg gaagaccgtc
     2101 catccctgct ggttgcggtg atgttcagcc ttatcattta cattgagaac cggatgtacc
     2161 gcacgccgcg taacctcaag aaactgaacg tcattgatga aggctggcgt ctgctggact
     2221 tcaaaaatca caaggtcggt gaatttattg agaaaggcta ccgtaccgcc cgccgtcata
     2281 ccggtgccta tatcaccatc acacagaaca tcgtcgactt tgactctgac aaggcctcca
     2341 gtgcggcccg tgcagcatgg ggtaactcct cctacaaaat tatcctcaaa cagagtgcga
     2401 aggagttcgc gaaatacaac cagctgtatc cggaccagtt cctgccactg cagcgtgaca
     2461 tgattggtaa gttcggtgcg gccaaagatc agtggttcag ttccttcctg ctgcaggtgg
     2521 aaaaccattc ctcctggcac cgcctgtttg tggacccgtt aagccgcgcc atgtacagct
     2581 ctgacggccc ggattttgag tttgtgcagc agaaacgtaa agaggggctg agtattcatg
     2641 aggcggtgtg gcagctggcg tggaagaagt cagggccgga gatggcttcg ctggaagcct
     2701 ggctggaaga acatgagaaa tacaggagtg ttgcatgacc acaacgcaaa aaacgactga
     2761 cgttacagcc cgtcgacgca gccactggtg gtggacggtg ccgggatgcc tggcaatggt
     2821 gttactgaac gcagccatca gttacggcat tgtcagactg aacgcaccgg tgactgccgc
     2881 cttcaacatg aaacagaccg tggatgcgtt ttttgacagc gccagtcaga aacaactgtc
     2941 agaagtacag tcaaaagccc tttcagcgcg ttttaacacg gcactggaag ccagcctgca
     3001 ggcatggcag cagaaacacc acgcggtcat tctggtgtcg cctgccgtgg tacagggggc
     3061 tccggatatc acccgggaaa tccagcagga tatcgcccgg cgaatgaggg cggagccatg
     3121 agatgccggg ggctgattgc cctgctgata tgggggcaga gtgtggccgc cgccgatctt
     3181 ggtacctggg gcgatctgtg gccggtaaag gagccggaca tgctgacggt gatcatgcag
     3241 cgcctgacgg cactggagca gtccggtgag atgggccgga aaatggatgc ctttaaggag
     3301 cgggtgatcc gcaacagtct gcggccccct gccgtgcccg gtatcggacg cacggagaaa
     3361 tacggcagcc ggttgtttga cccgtccgtc agactggctg cggatatccg ggataacgaa
     3421 gggcgggtgt ttgcccgtca gggtgaagtg atgaatccgc tgcagtatgt tccttttaac
     3481 cagacgctgt atttcatcaa tggcgatgat ccggcgcagg tggcctggat gaaacgccag
     3541 acgccgccca cactggagag caaaattatc ctcgtgcagg gcagtatccc ggagatgcag
     3601 aagtctctgg acagccgtgt ttactttgac cagaacggtg tgctctgcca gcgcctcggc
     3661 attgatcagg tcccggcacg ggtgagcgcc gttcccggcg atcgcttcct gaaggtggaa
     3721 tttattccgg cagaggaggg cagaaaatga agcgaagcct gtggctgctg atgttattcc
     3781 ttctcgccgg tcatgtccct gcagcgtctg cggattctgc ctgtgagggg cgttttgtaa
     3841 acccgatcac ggatatctgc tggagctgta ttttcccgct ctcgctgggc agtatcaaag
     3901 tcagtcaggg taaggtcccc gacacggcga acccgtcgat gcccattcag atttgtccgg
     3961 caccgccgcc gctgttcagg cgtatcgggc tggccattgg ttactgggag ccgatggcgc
     4021 tgacggacgt cacccgatca ccgggatgca tggttaacct gggcttcagc ctgccggctt
     4081 ttggtaaaac ggcacaggga acggcgaaaa aggatgagaa gcaggtgaac ggggcgttct
     4141 atcacgttca ctggtacaaa tacccgctga cgtactggct taacatcatc acatcgctgg
     4201 gctgtctgga aggtggtgac ctggatatcg cttatctttc tgaaatcgac cccacctgga
     4261 cggacagcag cctgaccacc attctcaatc cggaagctgt catctttgcc aatccgatag
     4321 cacagggagc ctgcgcagca gatgcgattg ccagcgcctt taatatgcct ctcgatgttc
     4381 tgttctggtg tgccggttcg cagggaagta tgtacccgtt caatggctgg gtgagtaatg
     4441 agtccagtcc gttgcagtcc tccctgctgg tcagtgaacg catggcgttc aagctgcacc
     4501 gtcagggcat gattatggaa accatcggga aaaataacgc cgtctgtaat gaatatccgt
     4561 ccccaatcct gcccaaagaa cgctggcgtt accagatggt gaatatgtat ccggacagcg
     4621 gacagtgcca cccgttcggg cgcagcgtga cccgctggga aaccgggaaa aatccgccca
     4681 acacaaagaa aaacttcggc tacctgatgt ggcgtaaacg taactgtgtc ttcctgtgag
     4741 gtgaatgatg aagctgagta tgaaatttct ggcagcactg ctgatgatgc tgaacggggc
     4801 ggttatggcg tcagaaaacg tgaacactcc tgaaaaccgc cagttcctga agcagcagga
     4861 aaatttaagc agacaactgc gtgaaaaacc tgaccatcag ctgaaagcct gggcggagaa
     4921 gcaggtgctg gaaaaccccc ttcagcgttc agataaccat ttcctggatg agctggttcg
     4981 taaacagcag gcttcgcagg acgggaaacc ccggcagggt gctctgtatt ttgtgtcgtt
     5041 ttccatttcc gaagaggggc tgaaacgaat gctgggcgaa acccggcact acggtatacc
     5101 cgccacactg cggggcatgg tgaacaatga cctgaagacc accgctgaag ccgtgctgtc
     5161 cctggtgaaa gacggcgcga ctgatggtgt tcagatcgac ccgacgctgt tttcacagta
     5221 cggcattcgc agtgtaccgg cactggtggt gttctgcagt cagggatacg acatcatccg
     5281 gggaaacctg cgggtcggcc aggcgctgga gaaagtggcc gccacgggtg actgccggca
     5341 ggtggctcat gatttactgg cagggaaagg agattccggg aaatgaaacg tattttacct
     5401 ctgatactgg ctctggttgc cggcatggca caggctgaca gcaacagtga ttaccgggcc
     5461 ggctctgatt ttgctcacca gatcaaagga cagggaagca gcagtattca gggctttaag
     5521 ccacaggaga gtatccccgg ctataacgcg aatccggacg agacaaaata ctacggtggc
     5581 gtgactgccg gcggggatgg cggcctgaaa aatgacggca ccacggaatg ggcgaccggt
     5641 gaaaccggaa aaaccatcac agagtcattc atgaacaagc cgaaagacat tctttcaccg
     5701 gatgcaccgt ttatccagac cggcagggat gtggtgaacc gggctgacag cattgtggga
     5761 aacaccgggc agcagtgcag tgcgcaggag attagccgga gtgaatacac gaattacacc
     5821 tgtgagcggg atttacaggt ggagcagtac tgcacacgaa ctgcccgtat ggagctgcag
     5881 gggagtacca catgggaaac ccggacgctg gagtatgaaa tgagtcagct acctgcacgt
     5941 gaagtgaatg gtcagtatgt tgtctccatt acctctcccg ttaccggtga aattgtcgat
     6001 gcgcattaca gctggagtcg tacttacctg cagaagagtg tacctatgac aattacagta
     6061 ctgggaaccc cactgagctg gaatgccaaa tactcagcag atgcctcatt tacaccagta
     6121 cagaaaacac tgactgccgg tgttgctttt acgtcatctc accccgtccg ggtcgggaat
     6181 acaaaattca aacgtcatac ggcaatgaag ctgcgtctgg ttgtcagggt aaaaaaagcc
     6241 tcgtacaccc cgtatgttgt ctggtctgaa agctgcccgt tcagcaagga gctggggaaa
     6301 ctgacaaaaa cagaatgtac ggaggctggc gggaacagga cgctggtgaa agacggtcag
     6361 tcatacagca tgtaccagag ctgctgggca taccgggaca cgtatgtcac acagtcagca
     6421 gacaagggaa cctgtcagac ctacaccgac aatccagcct gtaccctggt gtcacaccag
     6481 tgtgcctttt actccgaaga aggtgcctgt ctgcatgaat atgccacgta ctcctgtgag
     6541 tcaaaaacat ccgggaaagt gatggtctgt gggggagacg tcttctgtct cgatggcgaa
     6601 tgcgataagg ctcagagcgg gaagagtaat gattttgccg aagccgtttc tcagctggcg
     6661 gcactggccg cagcaggtaa ggatgttgcg gcgctgaacg gggtggatgt ccgtgctttc
     6721 accggtcagg cgaaattttg taagaaggct gcagccggat acagcaactg ctgtaaggac
     6781 agcggctggg gacaggacat cgggctggcc aaatgcagca gtgatgaaaa agccctggct
     6841 aaagcaaaat caaacaaact gaccgtcagt gtcggagagt tctgttcgaa gaaagtgctg
     6901 ggtgtctgcc tggagaaaaa acgcagttac tgtcagtttg attcgaagct ggcgcagata
     6961 gtccagcagc aggggcgcaa cggacagctg cgtatcagtt ttggcagtgc gaagcatcct
     7021 gactgtcggg ggattacggt tgatgagctg cagaaaattc agttcgacag actggatttc
     7081 actaacttct acgaagacct gatgaataac cagaaaatcc cggacagtgg tgttctgacg
     7141 cagaaagtga aagagcagat tgctgaccag ctgaaacagg caggacagta aggtagggaa
     7201 aataaccggg gaaaatgatg aaagtcatat tcacgtcgaa ccgttttatt gattttctta
     7261 tccggctttt gattacagcg attgtcattt ccccggtaat tatctggtcg tgggatacgg
     7321 taaaagaaac cactgcaggt gagatacttg ctgctgcttt cgttattttt tactccggtg
     7381 tgctcctgtt tattctgtat ttttgttttt ctgccctgac tgacctgcag aaacctgatg
     7441 aaagaaaaag tgatgaaagg aatgaagatg aataaagcat tactgccact gttactctgc
     7501 tgctttattt ttccggcgtc aggaaaagat gcaggctggc agtggtataa cgagaaaata
     7561 aatccgaagg aaaaagaaaa taaaccagta cctgcagccc cccgtcagga gccggacatt
     7621 atgcagaaac tggccgcact gcagacggca acaaagcggg cgctgtacga agccattctg
     7681 tatcccggcg tggataattt tgtgaaatat ttccggctgc agaattactg gactcagcag
     7741 gccgggcttt tcaccatgag cgccaaaaaa gccatgctgg cgcatcccga actggactat
     7801 aacctgcagt acagccatta caacggcacg gtccggaacc agctggcggc agaccaggtg
     7861 cagcagcgac aggccattgc gaaactggct gaacactacg gcatcatgtt cttttaccgg
     7921 gggcaggacc ccatcgacgg gcagctggcg caggtcatta atggcttcag ggatacctat
     7981 ggtctgagtg ttattcccgt ttccgtggat ggtgtgatta atccgctgtt gccggattcc
     8041 cggactgacc aggggcaggc gcagcgcctc ggcgtgaaat atttcccggc catgatgctg
     8101 gttgacccga aacagggcag tgttcgcccg ttatcatacg gctttatttc gcaggacgac
     8161 ctggcaaaac aattcctgaa cgtttctgaa gattttaaac ccaattttta atcgcggatt
     8221 tgatttatga gtgaagatta tttgaaactg tttacaggtg ttgttctgtt aatatttgtc
     8281 attattgccg gttatttctt ttctgagcgt aatgacagga aaatgtttct cctgagctca
     8341 ctggttttcc ttgttattaa tatcgcgtgt ttatatgtgc tgactgccag tctctggttt
     8401 ctgtgtggtg caattatgaa tcagggcgca gcaatggttg ttccaatggt tattggctca
     8461 ttaccgaacg ttacgagctt cgacgggttc agaagaatat ttatctgtat tatgttgtca
     8521 tcagtatggt ccggagtgat gtggtttttt ataagggggc ttatgacagg ctaagtcata
     8581 taaaacggta ataaaaacca tacaaaataa tgaggttgat ctgattaata tc
//
LOCUS       DBBGLQ010000152         8544 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.152, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000152 DBBGLQ010000000
VERSION     DBBGLQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.152"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..222
                     /locus_tag="ACIYSB_004796"
     CDS             <1..222
                     /locus_tag="ACIYSB_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGC5431666.1"
                     /translation="DLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELL
                     LEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            592..1416
                     /gene="gadX"
                     /locus_tag="ACIYSB_004797"
     CDS             592..1416
                     /gene="gadX"
                     /locus_tag="ACIYSB_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGC5431667.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVFVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQRLSNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1784..2512
                     /gene="gadW"
                     /locus_tag="ACIYSB_004798"
     CDS             1784..2512
                     /gene="gadW"
                     /locus_tag="ACIYSB_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGC5431668.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLYTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2875..5988)
                     /gene="mdtF"
                     /locus_tag="ACIYSB_004799"
     CDS             complement(2875..5988)
                     /gene="mdtF"
                     /locus_tag="ACIYSB_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGC5431669.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6013..7122)
                     /gene="mdtE"
                     /locus_tag="ACIYSB_004800"
     CDS             complement(6013..7122)
                     /gene="mdtE"
                     /locus_tag="ACIYSB_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGC5431670.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7230..7508
                     /locus_tag="ACIYSB_004801"
     CDS             7230..7508
                     /locus_tag="ACIYSB_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431671.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7509..8036)
                     /gene="gadE"
                     /locus_tag="ACIYSB_004802"
     CDS             complement(7509..8036)
                     /gene="gadE"
                     /locus_tag="ACIYSB_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGC5431672.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         2222 a         1983 c         2001 g         2338 t
ORIGIN      
        1 gacctctctg aacgtctgcg tctgcgcggc tggcaggttc cggccttcac tctcggcggt
       61 gaagccaccg acatcgtggt gatgcgcatt atgtgtcgtc gcggcttcga aatggacttt
      121 gctgaactgt tgctggaaga ctacaaagcc tccctgaaat atctcagcga tcacccgaaa
      181 ctgcagggta ttgcccagca gaacagcttt aaacacacct gataacataa cgttgtaaaa
      241 accgaatgcc cagcctttaa aaaaacagct gggcattcgg ttgcttatta atcgcaataa
      301 tatattggct gtctattcat cgtgttgata agatatatgt tatatgaatg tttattaaat
      361 gtagcgatta cctttaatgt cacgaaaagg gattatttgc ttactattaa tttccctgcg
      421 ttcttaaaca cgtttttgta ttataaatcc agtcatcctg cccgtagaac atcacacatt
      481 atcatcctgt tctcccgctt cgtttaaatt tatttatcaa tcaatttgac ttaagagggc
      541 ggcgtgctac attaataaac agtaatatgt ttatgtaata ttaagtcaac tatgcaatca
      601 ctacatggga attgtctaat tgcgtatgca agacataaat atattctcac catggttaat
      661 ggtgaatatc gctattttaa tggcggtgac ctggtttttg cggatgcaag ccaaattcga
      721 gtagataagt gtgttgaaaa ttttgtattc gtgtcaaggg acacgctttc attatttctc
      781 ccgatgctca aggaggaggc attaaatctt catgcacata aaaaagtttc ttcattactc
      841 gttcatcact gtagtagaga tattcctgtt tttcaggaag ttgcgcaact atcgcagaat
      901 aagaatcttc gctatgcaga aatgctacgt aaaagagcat taatctttgc gttgttatct
      961 gtttttcttg aggatgagca ctttataccg ctgcttctga acgttttaca accgaacatg
     1021 cgaacacgag tttgtacggt tatcaataat aatatcgccc atgagtggac actagcccga
     1081 atcgccagcg agctgttgat gagtccaagt ctgttaaaga aaaaattgcg cgaagaagag
     1141 acatcatatt cacagttgct tactgagtgt agaatgcaac gtgctttgca acttattgtt
     1201 atacatggtt tttcaattaa gcgagttgca gtatcctgtg gatatcacag cgtgtcgtat
     1261 ttcatttacg tctttcgaaa ttattatggg atgacgccca cagagtatca ggagcgatcg
     1321 gcgcagagat tgtcgaaccg tgactcggcg gcaagtattg ttgcgcaagg gaatttttac
     1381 ggcactgacc gttctgcgga aggaataaga ttatagagtt ttactcagac ataaaaaaaa
     1441 cccggcatag gggaccggga agaggatagt ctgccgtctc cagactaata aaccgttata
     1501 acactccctg ttggcacggg aaactttgtg ctctcagtaa gttaaatata acttttactg
     1561 gaaataagat cagccatttt tttataaaca taagctatac gctgtgcgaa aatatattcg
     1621 tgctgcattt acttattatc aattaactgt tatgcaaaac tactttgtgg ataaattttg
     1681 gtcctaccaa atctggcagt ttttgcgcta agaaacagtc tggcatcatt tcattagtat
     1741 actgaaattg aaataatcgc agtatgaaat ataagggata atcatgactc atgtctgctc
     1801 ggtgatcctc attcgtcgtt cattcgatat ttatcatgaa cagcaaaaaa tatcgctgca
     1861 taacgagagt attctgctgc tggagaaaaa tttggcagac gattttgcgt tttgttcacc
     1921 ggatacgcga cgactggata tcgatgagct gacagtttgc cattacttac aaaatattcg
     1981 tcagctacca cgcaatttag ggttacacag caaagaccgt ttgttaatta accagtcacc
     2041 ccccatgccg ctggtgacgg cgatttttga tagcttcaat gaatccgggg taaattcacc
     2101 gatactgagc aatatgctct acctttcctg tttatcgatg ttttctcata agaaagaact
     2161 gatcccctta cttttcaata gcatcagcac tgtttcagga aaagttgaac gccttattag
     2221 ctttgatatc gccaaacgtt ggtatctgcg cgatatcgcg gaaagaatgt ataccagcga
     2281 gagtctaatc aaaaaaaagt tgcaggatga aaatacctgt ttcagtaaaa tattactcgc
     2341 ctccaggatg tcgatggcca gacgattact cgagttacgt caaattcctc tgtatactat
     2401 tgcggaaaaa tgtggctata gcagtacatc gtactttata aacacatttc gacaatatta
     2461 tggtgtaacg ccacatcagt ttgcgcaaca ttcgccaggt accttttcct gacatatttc
     2521 gcatttgaat attggtcagg atctcacacc tgcttcatgt gaaactcttc cctgatgatt
     2581 tctgccgggc taccggctag ttctctttcg cagtttgcca ggcggtatcg gtattttcag
     2641 gaggaaaaga ctcggcatgt ttgggattat taagctgaca attcatacga ttacgggaat
     2701 gtgggtcagt attgtgttgt tcaaattgat gacgaatggc tggagtggtt tctactttca
     2761 gtgctgtgta ctaagcctgg tattcctgac ggttagctgg ttgttgagtg gtgaatggtt
     2821 agcaggaaag agtaaggctg aaccttcatg ttcaacctta ctctcattta cacgttacgc
     2881 ttttttaaag cgggcaaaga gatgttccac tacaacgaaa aagaccggaa cgaagtaaat
     2941 tgccagcact gttgcggcaa acatcccgcc catcacgccg gtacctaccg cgttttgcgc
     3001 gccagaaccg gcaccatgac tgataaccag cggcagcacg ccgagaataa aggccagaga
     3061 ggtcatcagg attgggcgta aacgcatccg cgccgcttcg atgattgcct ctatcggcgt
     3121 tttcccttct ttctgcatca tctcaacggc aaattcgaca atcaggatgg cgtttttggc
     3181 ggaaagcccg atggtggtca gcaaaccaac ctggaagtag acgtcattac ttaagccgcg
     3241 cagatcggtg gccagtaatg cgccaacgac gcctaacgga acaaccaaca tcaccgagaa
     3301 cggaattgac cagctctcat agagtgcggc gagggcgagg aacaccacga ccagtgaaat
     3361 cgcatacagc gcaggagcct gatttgagga taacgcttcc tgatacgata gtccggtcca
     3421 tgagtagccg acgcctgccg gaagtttagc gaccaggtct gccataaatt tcatggcgtc
     3481 accggtactt ttcccggccg ccgcttcacc taaaatctcc attgacggga tgccgttgta
     3541 gcgttccagt cgcggtgaac cataggtcca ttcggtagac gagtaggcag aaagcggtgc
     3601 catcgtgcca gaggcgttgc gtacatacca ttggttgatg ttatccggca acatacggaa
     3661 cggcgtgcct gcctggacat acactttttt cacccgcccc tggttgagga agtcgttcac
     3721 gtagctgctg ccgaaggcgg tggaaattgt ctggttgata tcagacagcg ccacgcccat
     3781 cgcttcagct ttcgcagcgt tgacgttcac tttgaacatc ggcgtatctt ccaggccgtt
     3841 cgggcgtacc ccggtgacct gattcggtga ttgcgctgcc agtgataaca gctcgtttcg
     3901 cgcctgggtt agtttttcgt gccccaggtt accgttgtcc agcagttcca tatcaaaacc
     3961 tgacgcggta cccagttcag ccaccgcggg taagttgaac gggaagacga cggctttatt
     4021 gatactgctt aacgcaatca ttgcccgctg aatgatcgcg gtaaccgagt tttcctcacc
     4081 gacacgttca gaccacggct tgagactgat aaacgccagg ccgttgtttt gcccctgacc
     4141 gctgaagcca aagccgccaa cggtaaacac cgactggaca ttatctttct ctttagtcag
     4201 ataataatcc gtcacctgtt gcagcacttt cgtggtgtta accatggtgg caccggaagg
     4261 taactgcgcg gtggtcataa ataccccctg atcctcttct ggtaagaaag aggtcggcgt
     4321 gcgcaggaac agcaccgcca tcccggcgca aatcagcagg tagaccacca tgtagcgacc
     4381 ggtacaacgc aacagcgagc gggtgctatc ggtatagtgt tgagttgatt tttcaaacag
     4441 cgtgttgaag cgtgcgaaca gggcgttagg tttgtgaccg ccttccggcg cggctttcag
     4501 aatggtggcg cacagggcag gggtcaggct cattgccaca aatactgaaa gcagcatgga
     4561 ggagatcagc gtgatggaga actggcggta gatctccccg gttgcaccgc tcataaaggc
     4621 catcggcata aacactgcgg aaagaacaac ggcaataccg accagcgcac gttggatctg
     4681 ccccatcgat ttatgcgtcg cttccttcgg cggtagctta tcttccgcaa tgacacgctc
     4741 gacgttctcc accaccacga tggcgtcatc caccagtaac cctatcgcca gcaccatccc
     4801 gaacatagtc aacgtgttga tggtgaaacc gaccgccgac aagatcgcaa acgtcccgag
     4861 aataaccacc ggtacggcaa tcgtcgggat gattgtggca cggaaattct gcaaaaacag
     4921 atacatgacc aggaagacta ggatgatagc ctcaaccagt gttttgaaaa cttcctgaat
     4981 agaaatttcg ataaacggcg tggtgtcgta aggataaacc gtcttcagac ttgccgggaa
     5041 ataggctgat aagcggttca gttcctcttt gactgcccgc gaggtatcca gggcgtttgc
     5101 tccggcagcc agtttgatgg cgatcccggc agcaggtttg ccgttatagc gcgccacggt
     5161 ggaataatct tccgccccaa gttcgacgcg agcgacatca cgcagcagca cttgcgaacc
     5221 atcttgctga actttcaaca ggattttgcc aaattcttcc ggcgtttgca gacgcgtctg
     5281 cacaatgatc gaggcgttta gctgctggtc tgccgcctgt ggcatgccac ccagttgacc
     5341 accggaaatc tggttgtttt gcaccttaat ctgggaaata acatcggaag gtaccaggtt
     5401 atatttattg agtttttgcg ggtccagcca gatacgcatg gcatactcgg aaccaaagag
     5461 ttgtacgcta ccaacgcccg cggtacggct tagcgggtct ttgatattag acgctacata
     5521 gtccgcgata tcgtactggt tgaggctgcc gttatcagaa ataaacgccg ctaccatcag
     5581 gatattactg ctcgacttat cgacgctaat cccctgctgc tgcactgctt caggtaatga
     5641 aggcatagcg agttgcagtt tattttgcac ttgaacctgt gcgatatcag gagatgtccc
     5701 agtctcgaag gtcagagtga tagaggcatt gcccgccgca tcactggttg aagacatgta
     5761 catcaggcca tcaagcccat tcatattttg ctcaatcacc tgagtgaccg agtcttctac
     5821 cgtttgcgca tcggcacctg gataggtagc gctgacggta atggtcggtg gcgcaatctg
     5881 cggatactgc gcaaccggta agttcatgat cgccagacca cctgcaagca tcataataat
     5941 ggcaagtacc caggcaaaaa ccgggcgatc aataaaatag ttagccatga aagtcccctt
     6001 aagcctgcaa cgttattgtt tcgattcggt gctggcgttt tcctggctgg aggaaattgc
     6061 tcgtgctttg atacccggac gaatgcgttg caaaccggaa acgatcaccc gatcgccagc
     6121 ctgcaagcca gaggtgacga cccactggtc gccgatggct ttgctggctt caatttcgcg
     6181 tagctgcacg acatcgtctt tatccagaat gagcgccgtt gctttaccct gggcgttgtg
     6241 ggtgacgcct tcctgcggca ccagtaatac attctggcgg ctaccttcat ccactaatgc
     6301 cgtgacgtac atgccaggca gcaagtcacc atttgggttg gggaaaatcg cccgtaacgt
     6361 cacggagccc gtggtttcat ccactgtcgg gtcggagaat ttcagcgtgc cggtctggct
     6421 gtagcgttta ccattttcca gattgagctg tactggcgta ctgccctgaa cctgtttgat
     6481 ttgcccactg gcgacctctt ctttcatgcg taagaaatct tgcaccgact gcgtgagatc
     6541 gacataaatc gggtccagac gttgtacggt aaccagcgaa tctgcctgat tagcggtaac
     6601 gagtgcgccg acggtcaccg acgatttccc gctgacgccc gtaatcggcg aggtgacatt
     6661 cgcgtattgc agattgatcg tcgcctgttc aacagccgct ttggcgacgg tgacattggc
     6721 ttctgcttca ttcaactggg tgcgcgcggt gtcgtaatcc tgacggctaa cgtagttggt
     6781 cttcagcaac gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg tagagagcgc
     6841 tttcgccagc gagcctttgg cggagtttag ctcggcctgt aaaggtgcag gatcaatctg
     6901 atacagcgaa tcgccctggt tcactttatc gccttcgata aagttgcgtt taatgataat
     6961 accgcccacc tggggacgta tctcggcaac ttcataagga acggttctac cgggcaattc
     7021 gctcaacaca ttgaccgaac cgggggagag tgtgacgaca ccgacctcag gcgtcatggc
     7081 ggcggcgttt tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc agaataacaa
     7141 cggtattaac agctttcttc ttctgttcat tttagtccct gaaaattctt gagaaataac
     7201 ggttctttaa attgcgcgcg aggagagata tgagggattt acagaccagc ggcatagtgg
     7261 ggttgtcagc cagtaaggtg ggatacaggc acagtgatcg acatggtgag gtcaacgaca
     7321 aaaaaaatgt tgccgttctg ccaacagttc ctgccagcat tcgggcaacg aagggttcta
     7381 ctggtggata cacataccag gggaataaag tcgttttagc ctctccttta gtaatcaccg
     7441 ggggtaatga aatcagcatc tgcatccctc gtcatgccag ccatcaattt cagttgctta
     7501 tgtcctgact aaaaataaga tgtgataccc agggtgacga tgtcgctcat acgtcttagt
     7561 ttcagcttgt acataatgtt gaatttctga atctttaatg tttgttgcga aatattgagc
     7621 gtcgacgtga tattgctttc atttttattt ttcagaatca gttgcataat tttgaactga
     7681 ttatgtgaaa aacataattg tgaattctta tggggcaagt gtttaccata agtaatctca
     7741 agaagatttt tgatactttc tttgcggcta acaaaataaa taggcgcttt agcttttagt
     7801 ttactgatgt gataaggtgc caaaacaata attctctcgg catctaattt ctccagaaat
     7861 ttaatcgctt cttcatcaag gatatgattg tgataggtat caatgataac cttgaagggt
     7921 ttattgccta cttttttgat ctctgacagg gaattgattt ttatcatttc gtgattatct
     7981 ttcaactgcc aaaagccctg taaaagaaaa gaatctttcg tcatgagaaa aatcataact
     8041 tgctccttag ccgttatcgt ttcgaatatg tcatcctttt cattatttac atccttgtcc
     8101 gaatcgttgt tcaatatagt aaacgcctat aagaacttta cccttcgtga actaatacgt
     8161 ctattaacac taatcaatac ttcagattta tccatcacgc actccatata ttgatcctaa
     8221 gtaagattcc tggttgttat cagcttgtaa atagcaaaca aaaagttaac gtttgcggat
     8281 tactgcccaa gaataagtta tcaataacca gtttgtgatc tctgaagaat attactaaag
     8341 ttaaaatgta atccgattta aatatcgagt ctccttgttt cgacttaagc tggcaattgg
     8401 attgccagct ttcttttaac gcattgaaat tacctgagta aataacgcat tcatgtcttc
     8461 aggtttttat atagcgtctg gagacacgga tatttatgca atgtaatcaa cgccagttgc
     8521 aaaaaaatga acccggatta ctta
//
LOCUS       DBBGLQ010000153         8357 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.153, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000153 DBBGLQ010000000
VERSION     DBBGLQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.153"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(88..294)
                     /locus_tag="ACIYSB_004803"
     CDS             complement(88..294)
                     /locus_tag="ACIYSB_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGC5431673.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            607..696
                     /gene="kdpF"
                     /locus_tag="ACIYSB_004804"
     CDS             607..696
                     /gene="kdpF"
                     /locus_tag="ACIYSB_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGC5431674.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            696..2369
                     /gene="kdpA"
                     /locus_tag="ACIYSB_004805"
     CDS             696..2369
                     /gene="kdpA"
                     /locus_tag="ACIYSB_004805"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGC5431675.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGTDSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQAASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2392..4440
                     /gene="kdpB"
                     /locus_tag="ACIYSB_004806"
     CDS             2392..4440
                     /gene="kdpB"
                     /locus_tag="ACIYSB_004806"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGC5431676.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWITVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGIIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMCLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4449..5021
                     /gene="kdpC"
                     /locus_tag="ACIYSB_004807"
     CDS             4449..5021
                     /gene="kdpC"
                     /locus_tag="ACIYSB_004807"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGC5431677.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5014..7698
                     /gene="kdpD"
                     /locus_tag="ACIYSB_004808"
     CDS             5014..7698
                     /gene="kdpD"
                     /locus_tag="ACIYSB_004808"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGC5431678.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDNRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7695..>8357
                     /gene="kdpE"
                     /locus_tag="ACIYSB_004809"
     CDS             7695..>8357
                     /gene="kdpE"
                     /locus_tag="ACIYSB_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGC5431679.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGY"
BASE COUNT         1749 a         2186 c         2432 g         1990 t
ORIGIN      
        1 gtctaaatat tcctctgaac atcttcctga tacaaaaaac cgccagcgtt tccgctggcg
       61 gttttcaatg tattcaaaac tcagcaatca ataaaaatca ccagttgcct tttcggcctg
      121 atccatccac accggtttat cgctggtttt cgaccagacg cgatgcaggt agctgtaaaa
      181 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
      241 ataagtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
      301 ctcccccttt tgtatctacc cggtgaatgg caccggaaaa atgaatttgt ttatctgatg
      361 aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt
      421 gatggtttat ttacattcat cctgtaatta agttacacaa aagttaaatt aatactaaac
      481 attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
      541 ttgcgaaatc tttgcagcca gaattctacc cttccggtat cacttttagg ccactggagg
      601 tgcactgtga gtgcaggcgt gataaccggc gtattgctgg tgtttttatt actgggttat
      661 ctggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
      721 tcgccacgtt tttactggtg ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
      781 ggctgattaa tgacattcct cttcccggta caacgggcgt tgagcgcgta ctttttcgcg
      841 cacttggcgt ctctgaccgt gagatgaact ggaagcaata tctttgtgcc attctcggcc
      901 tgaacatgct ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
      961 ttaatccaca gcagttgcca gggctgtcgt gggatctggc gctgaatacc gccgtcagct
     1021 ttgtcaccaa taccaactgg caatcttata gcggtgaaac cacgttgagc tatttcagcc
     1081 agatggcggg cttaacggtg caaaactttc tttctgccgc cagcgggatt gcggtgattt
     1141 ttgccctcat ccgtgcgttt acccgccaga gcatgagcac gctcgggaat gcctgggtcg
     1201 atctgctacg catcacgtta tgggtgctag tccctgtggc gttgttgatt gcactgtttt
     1261 ttattcaaca aggtgcgctg caaaactttc tgccttatca ggctgtgaat accgttgaag
     1321 gagcgcaaca gctgttaccc atggggcctg tagcttctca ggaagcgatc aagatgctcg
     1381 gtactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
     1441 cactgaccaa cttcgtgcag atgctggcga tcttcttgat cccaacggcg ctgtgctttg
     1501 cctttggtga agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag
     1561 tgatttttgt catctgcgta ggcgtggtga tgtgggcaga agttcagggt aatcctcatc
     1621 tgctggcact gggcacggac agcagcatca atatggaagg taaagagagc cgtttcggcg
     1681 tgctggtcag tagcctgttt gcggtcgtga cgacggcggc ttcctgtggc gcggtgattg
     1741 cgatgcatga ttcgtttacc gctctcggtg gcatggtgcc gatgtggctg atgcaaattg
     1801 gtgaagtggt gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc
     1861 tggcggtgtt tattgccggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa
     1921 tcgacgtacg cgagatgaaa ctgactgcac tggcaattct ggttaccccg acgctggtgc
     1981 tgatgggcgc ggcgttggcg atgatgaccg acgccggacg tagcgccatg ctcaaccctg
     2041 gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcatc cgccgctaac aacaacggca
     2101 gcgcctttgc cggattaagc gccaactctc cgttctggaa ctgtttactg gcgttctgca
     2161 tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc aattgccggt tcgctggtga
     2221 gtaaaaagag ccaagccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg
     2281 gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc
     2341 ttggtccggt ggcggaatat ctctcctgat gatattgagt gagcactgaa tatgagtcgt
     2401 aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa
     2461 aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt
     2521 ctgctgacca cctgtattag catcgcgatg gcaagcggtg cgatgcccgg caatgcgctg
     2581 tttagcgcgg ccattagcgg ttggctgtgg atcaccgtac tgttcgctaa tttcgccgag
     2641 gcgctggcag aaggccgcag taaagcgcag gccaacagtc tgaaaggggt gaaaaaaact
     2701 gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcctgcc
     2761 gaccaacttc gtaaaggcga tatcgtactg gtagaagctg gcgatattat cccctgcgat
     2821 ggtgaagtta ttgaaggggg tgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca
     2881 ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ccggcggcac gcgtattctt
     2941 tctgactggc tggtgattga gtgtagcgtt aaccccggcg agacatttct ggatcggatg
     3001 atcgcgatgg tggaaggcgc acagcgacgc aaaacgccga acgaaattgc cctgaccatt
     3061 ctgctgattg ccctgactat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc
     3121 gcgtggggcg gtaatgcagt cagcgtaacg gtactggtgg cgctgctggt ctgtctgatc
     3181 ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag ccggatgcta
     3241 ggcgcgaatg tgattgccac cagcggacgt gcagttgaag cggcaggtga cgttgacgtt
     3301 ctgctactgg ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc
     3361 cccgcgcagg gcgtggatga aaaaacgctg gctgacgccg cacaactggc ttcgctggct
     3421 gatgaaacgc cggaaggccg cagtattgtg atcctcgcca agcagcgttt taacctgcgc
     3481 gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca aagccggatg
     3541 agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgtcgc
     3601 catgttgagg ctaacggtgg tcacttccct accgatgttg atcaaaaagt cgatcaggtt
     3661 gcgcgtcagg gagccacgcc gctggtggtg gtggaaggtt ctcgtgtgct gggcattatt
     3721 gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg
     3781 ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc gattgctgcg
     3841 gaagcgggtg tcgatgattt tctcgccgaa gcgacaccgg aggccaagct ggcattgatc
     3901 cgtcagtatc aggcggaagg tcgtttggta gcgatgaccg gcgacggcac caacgatgct
     3961 ccggcgctgg cgcaggcaga tgtcgcggtg gcgatgaact ccggcaccca ggcggcgaaa
     4021 gaggcgggca atatggtcga tctcgactct aacccgacca agttgatcga ggtggtgcac
     4081 attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat tgccaacgat
     4141 gtggcgaaat acttcgccat tattccggcg gcattcgcgg caacgtatcc gcagttaaat
     4201 gcgctgaaca tcatgtgcct gcattcgccc gactccgcaa tcctcagtgc ggtgattttc
     4261 aacgccttga ttatcgtctt tttgattccc ctggcgttaa aaggcgtgag ttataaaccg
     4321 cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg
     4381 gtgccgttta tcggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga
     4441 ggtttaccat gagtggatta cgtccggcat tatcaacatt tatctttctg ttattgatta
     4501 ctggcggcgt ttacccgctg ctgaccaccg tactggggca atggtggttt ccctggcagg
     4561 ccaatggttc gttgattcgt gaaggtgata cggtgcgcgg ttcggcatta atcgggcaga
     4621 attttaccgg caacggctat tttcatggtc gcccgtcggc aacggcagaa atgccctata
     4681 atccacaggc ttctggcggg agcaatctgg cggtcagtaa ccctgagctg gataaactaa
     4741 tagccgcacg cgttgctgca ttacgggccg ctaacccgga tgccagcgcg agcgttccgg
     4801 ttgaactggt gacggcatcg gcaagcgggc tggacaataa tatcaccccg caagcggcgg
     4861 cctggcaaat cccacgcgtg gcgaaagcgc gtaatctcag cgttgaacag ctcacgcaac
     4921 tgatcgcaaa atacagccaa caaccgctgg tgaaatatat cggccagccg gttgtcaaca
     4981 ttgttgaact caatctggcg ctggataaac ttgatgaata acgaaccctt acgtcccgac
     5041 cccgatcgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa agttttcttc
     5101 ggtgcctgtg caggcgtcgg gaagacctgg gcgatgctgg cagaagccca gcgactgcgg
     5161 gcgcaagggc tggatattgt ggttggcgtg gtagaaaccc acgggcgaaa agataccgcc
     5221 gccatgctgg aagggctggc tgttctgccg ttaaaacgcc aggcgtaccg tgggcggcat
     5281 atcagcgagt ttgatctcga tgccgccctc gcccgccgcc cggcgctgat cttaatggac
     5341 gaactggcgc acagtaatgc gccaggttcc cgtcatccca aacgctggca ggatatcgaa
     5401 gaactgctgg aagctggcat tgatgttttc actaccgtca acgttcagca tctggaaagt
     5461 ctgaatgatg tggtcagcgg cgtcaccgga attcaggtac gggaaaccgt gcccgatcct
     5521 tttttcgatg ccgccgacga cgtggtgctg gtggacttgc ccccggacga tctgcgccag
     5581 cggctgaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat tgaacatttt
     5641 ttccgcaaag gtaatctgat cgccctgcgc gaactggcac tgcgccgtac tgccgatcgc
     5701 gttgatgagc aaatgcgcgc ctggcggggg catcctggcg aagagaaagt gtggcacacg
     5761 cgcgacgcga tccttttatg catcggccat aacaccggca gcgaaaaact ggtccgcgca
     5821 gcggcgcggc tggcgtcacg gctgggtagc gtctggcacg cggtgtatgt tgaaacccct
     5881 gccctgcacc gcttaccgga aaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg
     5941 caggaactgg gcgcggagac ggcaacactt tctgatccag cggaagagaa agcggtagtg
     6001 cgttatgccc gtgaacataa tctcggcaag attattctcg gtcgcccggc ctcgcgccgc
     6061 tggtggcgtc gggaaacgtt tgctgaccga ctggcgcgca tcgcccccga tctcgatcag
     6121 gtgctggtcg cgcttgatga accacccgcc cgcacgatta acaacgcgcc ggataaccgc
     6181 tcttttaaag acaagtggcg tgtacaaatt cagggatgcg tggttgccgc cgcgttatgc
     6241 gccgttatca ccttaattgc catgcagtgg ctgatggcgt ttgatgccgc caacctggtg
     6301 atgctgtatc tgcttggcgt ggtggtggtg gcgctatttt atggacgctg gccttcagtg
     6361 gttgccaccg tcattaatgt agtgagtttc gatctctttt ttatcgcccc acgcggcacg
     6421 ctcgccgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac cgtcgggctg
     6481 gtgatcggga accttactgc tggcgtgcgt tatcaggcgc gggtagcccg ttaccgcgag
     6541 caacgcacac ggcacttata tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag
     6601 gatatcgctg ccaccagcga acaatttatt gcctccacgt ttcatgcccg cagtcaggtg
     6661 ttgttgcccg atgacaacgg taaattgcag ccgttaacac atccgcaagg aatgacgccg
     6721 tgggacgatg ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg cgcgggcacc
     6781 gacacgttac ccggtgtacc gtaccagatt ttgccgctaa aaagcggcga gaaaacctac
     6841 gggctggtgg tggtggagcc ggggaatctg cgccagttga tgatcccgga acagcagcgc
     6901 ctgctggaga cgtttacgct gttagtcgcc aatgcccttg agcggctgac gctaaccgcc
     6961 agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggcg
     7021 gcgctttcgc atgatttacg cacgccgctt acggtgctgt ttggtcaggc agaaatctta
     7081 acgctcgatc tggcaagcga aggatcaccc cacgcccgcc aggccagcga gatccgtcag
     7141 catgtgctga acactacccg actggtgaat aatctactgg atatggcgcg aattcagtcc
     7201 ggcggcttta atttgaagaa agagtggtta acgctggaag aagtagtcgg cagcgcgctg
     7261 caaatgctgg aaccgggttt atcgtcgccc atcaatcttt ctctgccaga accgctgacc
     7321 ttaatccacg ttgacgggcc actctttgaa cgggtgctga ttaatctgct ggagaacgcg
     7381 gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga gggcgaaaat
     7441 ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga gcagacgata
     7501 tttgataagt ttgctcgcgg gaataaagag tcggcagtac cgggggtagg gcttggactg
     7561 gcaatttgtc gggcgatagt ggatgtacac gggggcacta ttaccgcgtt caaccgaccg
     7621 gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ctgcccctga acttgaagaa
     7681 tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct attcgtcgct
     7741 ttctgcgcac ggcgctggag ggcgacggga tgcgcgtctt tgaggccgaa acgctgcaac
     7801 gcggcttgct ggaagcggca acccgtaagc cagatttgat tattctcgat ctcggcctgc
     7861 ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcgcg gtgccggtga
     7921 ttgtgctttc cgcacgcagc gaagagagcg acaaaatcgc cgcgctggat gccggagcgg
     7981 atgattatct gagtaagccg tttggcattg gcgaattgca ggcccgtctg cgcgtcgcat
     8041 tacgccgcca ctctgccacc accgcgcccg atccgctggt aaaattttcc gatgttaccg
     8101 tcgatttagc cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg
     8161 agttccgcct gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc
     8221 tccttaacca ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata
     8281 tgggacatct gcgacaaaaa ctggaacagg atcccgcccg cccacgccat ttcattactg
     8341 aaaccggtat tggctat
//
LOCUS       DBBGLQ010000154         8323 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.154, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000154 DBBGLQ010000000
VERSION     DBBGLQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.154"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..481
                     /locus_tag="ACIYSB_004810"
     CDS             <1..481
                     /locus_tag="ACIYSB_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HGC5431680.1"
                     /translation="FINRFNTNKWLIDKNNNFFIQLIDQALRSTDDMIKANVWHLYKE
                     WIRSDDVSPIFIETEDNLRTFNTNELTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNP
                     TKDTNWNSTYIYKSRHEMLPVNLTQETLFSSKSHGKYALFPIFTASWRAHRIMNKGV"
     gene            704..1201
                     /gene="ubiC"
                     /locus_tag="ACIYSB_004811"
     CDS             704..1201
                     /gene="ubiC"
                     /locus_tag="ACIYSB_004811"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGC5431681.1"
                     /translation="MSHPALTQLRALRYCKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1214..2086
                     /gene="ubiA"
                     /locus_tag="ACIYSB_004812"
     CDS             1214..2086
                     /gene="ubiA"
                     /locus_tag="ACIYSB_004812"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGC5431682.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2241..4664)
                     /gene="plsB"
                     /locus_tag="ACIYSB_004813"
     CDS             complement(2241..4664)
                     /gene="plsB"
                     /locus_tag="ACIYSB_004813"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGC5431683.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4835..5203
                     /gene="dgkA"
                     /locus_tag="ACIYSB_004814"
     CDS             4835..5203
                     /gene="dgkA"
                     /locus_tag="ACIYSB_004814"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGC5431684.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5313..5921
                     /gene="lexA"
                     /locus_tag="ACIYSB_004815"
     CDS             5313..5921
                     /gene="lexA"
                     /locus_tag="ACIYSB_004815"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGC5431685.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5994..7319
                     /gene="dinF"
                     /locus_tag="ACIYSB_004816"
     CDS             5994..7319
                     /gene="dinF"
                     /locus_tag="ACIYSB_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGC5431686.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARTLVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPVVGVWCYLLDGMFIGATRATEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7435..7644
                     /gene="yjbJ"
                     /locus_tag="ACIYSB_004817"
     CDS             7435..7644
                     /gene="yjbJ"
                     /locus_tag="ACIYSB_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGC5431687.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7686..8201)
                     /gene="zur"
                     /locus_tag="ACIYSB_004818"
     CDS             complement(7686..8201)
                     /gene="zur"
                     /locus_tag="ACIYSB_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGC5431688.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1903 a         2028 c         2255 g         2137 t
ORIGIN      
        1 ttttattaac agatttaata cgaataaatg gctaattgat aaaaataaca atttttttat
       61 ccaacttatc gatcaggcct tacgatcaac ggatgatatg atcaaagcaa atgtttggca
      121 tctttataaa gagtggattc gtagtgatga tgtttcacct atatttatag aaactgaaga
      181 taatttaaga acctttaaca cgaatgaatt aacacgaaac gataatatct ttatcctgtt
      241 ctcctcagtc gatgatgggc cagttatggt ggtaagctcc cagcgcttac atgatatgtt
      301 gaatcctaca aaagatacca attggaattc cacgtatatc tacaaatcca gacatgagat
      361 gttgcctgtt aatcttactc aggaaacact tttcagctcc aaatctcatg gtaaatatgc
      421 gcttttcccc atttttactg cgagttggcg agctcatcgt ataatgaata agggtgttta
      481 agtaaaggaa aacatcaccg ttcctggcat cctggacggt gatgccccta cggttgccct
      541 cgccagcacg ggcatcggta aagcgtaagg ttcaacatcg ttttaccact tcatgcgatt
      601 gttgcgtttt tgttgcgtat tagatcactt aatttgcttt acatctcccg taaacacttt
      661 tctgcgatac aatgccttta cgttatgtaa cggagagttc ggcatgtcac accccgcgtt
      721 aacgcaactg cgtgcgctgc gctattgtaa agagatccct gccctggatc cgcaactgct
      781 cgactggctg ttgctggagg attccatgac aaaacgtttt gaacagcagg gaaaaacggt
      841 aagcgtgacg atgatccgcg aagggtttgt cgagcagaat gaaatccccg aagaactgcc
      901 gctgctgccg aaagagtctc gttactggtt acgtgaaatt ttgttatgtg ccgatggtga
      961 accgtggctt gccggtcgta ccgtcgttcc tgtgtcaacg ttaagcgggc cggagctggc
     1021 gttacaaaaa ttgggtaaaa cgccgttagg acgctatctg ttcacatcat cgacattaac
     1081 ccgggacttt attgagatag gccgtgatgc cgggctgtgg gggcgacgtt cccgcctgcg
     1141 attaagcggt aaaccgctgt tgctaacaga actgttttta ccggcgtcac cgttgtacta
     1201 agaggaaaaa aatatggagt ggagtctgac gcagaataag ctgctggcgt ttcatcgctt
     1261 aatgcgtacg gataagccaa ttggcgcgtt actgctgctc tggccaacat tatgggcgtt
     1321 gtgggtggcg acaccgggcg ttccccagct ctggatcctg gcggtgtttg tcgcgggtgt
     1381 ctggctgatg cgcgctgccg gatgtgtggt gaatgattat gctgaccgca agtttgatgg
     1441 tcatgttaag cgcacggcga accgaccact tcccagcggc gcggtaacag agaaagaggc
     1501 gcgcgcgctg tttgtcgtgc tggtactgat ttcgttttta ctggtgctga cgctgaatac
     1561 gatgaccatt ctgttgtcga ttgccgcgct agcgctggcg tgggtgtacc cgtttatgaa
     1621 gcggtatacc catctaccgc aagtggtgct gggcgcggcg tttggctggt cgattccaat
     1681 ggcttttgcc gctgtgagtg agtcggtgcc attgagttgc tggttaatgt tcctcgccaa
     1741 tattctctgg gcggtggctt acgacacgca gtatgcgatg gttgaccgcg atgatgatgt
     1801 gaagattggc attaaatcca cggcaatcct gttcggccaa tacgataaat tgattattgg
     1861 tattttgcag attggcgtac tggcactgat ggcgatcatc ggtgagttaa atggcttagg
     1921 ctggggatat tactggtcaa ttctggtggc tggcgcgctg tttgtttatc aacaaaaact
     1981 gattgccaac cgcgagcgtg aagcctgctt taaagcattt atgaataata actatgttgg
     2041 tctggtacta tttttagggc tggcaatgag ttactggcat ttctgatgat gtaaaaaagc
     2101 cggatgatca tccggctttc ttctgggttg cctgatgcgc ggcgcttctc aggcctacac
     2161 aacacatcgc aatttattga atttgcagat tatggaaggc cggataaggc gttttcgccg
     2221 catccggcaa ttctctctga ttacccttcg ccctgcgtcg cactctcaat cgtcaaacgc
     2281 acgtctgatg taatcaactc cgccagcaac tgataaacct tcatcgtttc tgccggttcg
     2341 gcatcgccgc tatcgctgat atacccttca tcacgcagtg tcagcaccag agaactgaac
     2401 accgccttgt cgaagaactc cggcgcgttg atgccgtgca gcacggagag acgttgcgcg
     2461 acggtgcggc tctctttctc cagcgtaccg cggttgatcg acgggttggc actcaacaac
     2521 cagaaggtga tggcataacg ttgcagcgtt tcgcgcgcgc ctgcggccag cagctgtagc
     2581 gtgcgagaat gcgccgggtt gatatgcaac tcatcatctt gcagggtaat cagcccctga
     2641 cgttgcatct catttgccag cgcatcaata acgtccggca actcgtcgcg atcccagcgc
     2701 aggaacagct ccgctttcag cattgggtaa agcacattga cgtgctccat caatacgtcg
     2761 cgggagatgt ggcgatgctg ggtgacgatt gccgccatca gcgaaggcag caccaacata
     2821 tgcgcaatgt tgttgcgata gtaggtcatc agcaccgctt gctcgcgcgg cagaatgatg
     2881 atgtcgccga ttgtgtcttt ctcgacttca aacttgttca tttgcagcgc gtgatcgata
     2941 agctcgctgg cgctggctga aggaacggta gagtccgtgg agtagggcac gttgcgcatc
     3001 agatccaggt agcagttgag ttgctcggtt aactgctcgc gggtgagtga gcgctgacgt
     3061 gatgccagta gcgcagtaca gcacaggttc atggcgtttg ccgcgcctgc gttgttaatg
     3121 cgtaccatca gatcggcagc aatattattg accgtcggcg ttaaccatgc cggacgcacc
     3181 gcttcgatgg gatcgataga ttcacgccag tcaggtacat gctggttaag gtaggtcatc
     3241 aacggcattg gttcaccgaa gttgacgtaa ccctgaccga gattacgcag cttgcttaaa
     3301 ccgcgcagca tctgcggcag gctctctttc tctttcgtcg cgccgcgcag ttctttggcg
     3361 taagtaccca cttccatgac gtgctcataa ccgatataga tcggaatcag cgtaatcgga
     3421 cgcgtgccgc cacgcagcat cgcctgaatg gtcatcgaca gcgtaccagt tttcggatcc
     3481 agcaaacgcc ccgtacggga acgaccgcct tccacgaagt actcgacgga ataaccacgg
     3541 ctgaacagtt cgccgagata ctcccggaaa acggtggaat aaagtttatt gcctttaaac
     3601 gtacggcgaa taaagaacgc ccccagacgg cggaaaatcg gcccggcagg ccagaaattc
     3661 aggttgatcc cggcggcgat atgcggcggc accagcccct ggtgatacag cacgtaagaa
     3721 agcagcaggt agtccatgtg actgcggtgg caaggcacat ataccagctc atgaccgtcg
     3781 tgggccagct ggcgaacgcg ctcagcgtta tggacgttga tgccctggta aagtcggttc
     3841 caggtgaagc ccagaatacg gtcagtcagg cgaatcatct cgtaagagaa attcgccgca
     3901 atctcttcca tcagtgcaat cgcgttctgc tgcgcttttt catgggagat ttttttgctg
     3961 cgcgcttcat cttctaccgc tttggcaatg gcgcgggagg cgagcagctt attaaacaga
     4021 tcctgacgag caggcaaacg tgggcctacg gcagccagac gttgacgggc aaagtgcata
     4081 cgcgccacgc gcgccagttt ctgagcgata gttttatccg tgccgtgttc atccgccata
     4141 cggcgcagcg aaactgacgg cgagaaacgc acaaaactgt cgcgaccgag ccacagtaca
     4201 gcgaaaaatt tctgtacgcc gttaagcata cgcagcggcg ggttcacttc gcctttttca
     4261 cgccccggcg cgcgaccaaa catcaccgac actggcacca tctgcacatc cagatttggg
     4321 ttgctacggt gcaaatcgag atagtcgtgg aacagcttaa tagactcttc tttcggcgtg
     4381 taataggtga acacacgcgg cccgccgtga atgaacacat agcgcggcag tagcgtgccg
     4441 tcgatttcca gcggctctaa cgggtcaggc aagtcatgtg ccagacactg ggcgcgcaac
     4501 gtcagcaaat ctgctttcga gttgtacggt aaaacgtaca taattggacg agaggtatcc
     4561 agccccagtt ccggggcagg atctgccgga atagacttgc tttttaccag gatgcttaat
     4621 ggtaaattca gtaatttgta gtaaattcgt ggccagccgg acataaacga tgtaaagcct
     4681 ctggttaata atgcaaatgc gcggcaagga tagcagaaag tcatgggaaa ttctgtggta
     4741 tccgctcatg tttcgcgcgg cgctacgcaa acccgaatca tcggatttaa cggtacactg
     4801 atattgacgc tcataatgta aaaaggttct ttcaatggcc aataatacca ctggattcac
     4861 ccgaattatc aaagctgctg gctattcctg gaaaggttta cgcgctgcat ggatcaacga
     4921 agcggcattc cgtcaggaag gcgtagcggt attgttggcg gtggtcatcg cctgctggct
     4981 ggatgtggac gcgattaccc gcgtgctgct tatcagctcc gtgatgctgg tgatgattgt
     5041 ggaaatcctc aatagcgcca tcgaagcagt ggttgaccga attggctctg aataccatga
     5101 gctttccgga cgcgcaaaag atatgggatc cgctgcggtg ctgattgcca ttatcgtcgc
     5161 cgtgattacc tggtgcattc tgttatggtc gcattttgga taacccttcc agaattcgat
     5221 aaatctctgg tttattgtgc agtttatggt tccaaaatcg ccttttgctg tatatactca
     5281 cagcataact gtatatacac ccagggggcg gaatgaaagc gttaacggcc aggcaacaag
     5341 aggtgtttga tctcatccgt gatcacatca gccagacagg tatgccgccg acgcgtgcgg
     5401 aaatcgcgca gcgtttgggg ttccgttccc caaacgcggc tgaagaacat ctgaaggcgc
     5461 tggcacgcaa aggcgttatt gaaattgttt ccggcgcatc acgcgggatt cgtctgttgc
     5521 aggaagagga agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa ccacttctgg
     5581 cgcaacagca tattgaaggt cattatcagg tcgatccttc cttattcaag ccgaatgctg
     5641 atttcctgct gcgcgtcagc gggatgtcga tgaaagatat cggcattatg gatggtgact
     5701 tgctggcagt gcataaaact caggatgtac gtaacggtca ggtcgttgtc gcacgtattg
     5761 atgacgaagt taccgttaag cgcctgaaaa aacagggcaa taaagtcgaa ctgttgccag
     5821 aaaatagcga gtttaaacca attgtcgttg accttcgtca gcagagcttc accattgaag
     5881 ggctggcggt tggggttatt cgcaacggcg actggctgta acatatctct gagaccgcga
     5941 tgccgcctgg cgtcgcggtt tgtttttcat ctctcttcat caggcttgtc tgcatggcat
     6001 tcctcacttc atctgataaa gcactctggc atctcgcctt acccatgatt ttctccaata
     6061 tcaccgttcc gttgctggga ctggtcgata cggcggtaat tggtcatctt gatagcccgg
     6121 tttatttggg cggcgtggcg gttggtgcaa cggcgaccag ctttctcttt atgctgttgc
     6181 tgtttttacg catgagcacc accgggctga ctgcgcaggc ttatggtgcc aaaaatcctc
     6241 aggcattagc ccgtacgctg gtgcaaccgt tgctgttggc gttgggggct ggggcgttaa
     6301 ttgcgctgct gcgtacgccg attatcgatc tggcgctgca tattgttggc ggtagtgagg
     6361 cagtcctgga acaggcgcgg cgctttcttg aaatccgctg gttaagcgca ccggcgtcgc
     6421 tggcgaatct ggtattactc ggttggttac tcggcgtgca atatgcccgt gcgccagtaa
     6481 ttttgttagt ggtcggcaat atcctcaaca ttgtgctgga tgtctggctg gtgatggggc
     6541 tgcatatgaa cgtgcagggc gcggcgctgg cgacggttat tgcggaatat gcaacattgc
     6601 tgattggtct gctaatggtg cgtaaaatcc tcaaactacg cggaatttcc ggcgaaatgc
     6661 tgaaaactgc ctggcgagga aacttccgtc gcttgctggc gcttaaccgc gatatcatgc
     6721 tgcgttcgct gttgttgcaa ctctgtttcg gcgcgatcac cgtacttggc gcgcgactgg
     6781 ggagtgacat tatcgctgtt aacgcggttc tgatgacgct actcaccttt accgcctatg
     6841 cgctggatgg ttttgcctac gcggttgaag cgcactccgg tcaggcatac ggtgcgcgcg
     6901 acggtagcca gttgctggat gtctggcggg cagcgtgccg ccagtcgggg atcgtagcgt
     6961 tactgttttc ggtggtttat ttgctggctg gggaacacat cattgcgtta ctgacgtcgt
     7021 taacccagat tcagcagctg gctgaccgct atcttatctg gcaggtgatt ttgccggtgg
     7081 ttggcgtctg gtgttatctg ctggacggca tgtttatagg cgcaacgcgt gccaccgaaa
     7141 tgcgtaacag tatggcggtg gccgccgcag gttttgcgct gacgctcctt acgctgccgt
     7201 ggctgggtaa tcatgctttg tggctggcat taaccgtctt tctggcgttg cgcgggcttt
     7261 ctctggcggc tatctggcgg cgtcactggc gcaatggtac ctggtttgcc gcaacgtgac
     7321 ggttaaaaat tctgaataaa taatcctaag ccaaattgct gactacactt aatctcacgt
     7381 tcagaagaaa agtgaacgta ctctcattca caacctaacg atgaggtctt gattatgaat
     7441 aaagatgaag ccggcggtaa ctggaaacag tttaaaggta aagtgaaaga gcaatggggc
     7501 aaactgaccg atgatgatat gacgatcatt gaaggtaaac gtgatcaact ggtcggtaaa
     7561 atccaggaac gttatggtta tcagaaagat caggcagaaa aagaggtcgt ggattgggaa
     7621 acccgcaatg aatatcgctg gtaattaatc cctcctgccc gacgtgtaca aggatgtacg
     7681 ccctcttaac gcggtttctt tttcacctgc acagagtgat catgctggca ctgttcagga
     7741 tgacgacacg cttccacttc tacacatgcc gcacagagcc catgtgcttc aatcacatta
     7801 tgccgcaggg caaaccccat ttttgccgcc agcgtatgca taatgtcttc cacgccttct
     7861 gcacactctt ctttcactgc gccgcagcga tcgcaaataa acatggctga cgtatgggtg
     7921 ggctgatcga acagatgaca gagcacataa ctgttggtgg attccacctt atgcacaaaa
     7981 ccttgctcaa gcagaaaatc cagcgcgcga taaaccgttg gcggcttggc ttgcggttca
     8041 gcttcgcgca gtaaatcaag cagatcataa gcgctgatag cgccatcctg cagactcatc
     8101 aggcgcaaca cttccaggcg ctgtggggtc aggcgcacat tacgctgcgc gcagattttt
     8161 tcagcctgcg ctaataactc ctgcgttgtg gtcttttcca tcgggcacct caaagtgggg
     8221 gttaagaatg tccttacttt accatgttcc aggaaaaaca ccgagaaccc ttattgttgc
     8281 cgtaatgttg attttctgtt ttgtaggtaa ggtgttatgt tgc
//
LOCUS       DBBGLQ010000155         8256 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.155, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000155 DBBGLQ010000000
VERSION     DBBGLQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.155"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(106..849)
                     /locus_tag="ACIYSB_004819"
     CDS             complement(106..849)
                     /locus_tag="ACIYSB_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431689.1"
                     /translation="MLNIKKYNLILLSTVLLLLLIAILVGVKILFSGSKGWEWVTLIG
                     TLCSAVGTLGTLWVAYQAYLKAPEWLSQKHYDVVYGVIEKAIYQDLNKVRTSTLILKH
                     QLVTFSKKYRNHLKNETIPQDSHDEFMKETDALVSDLFFTSYSVINSLKSIDRTNYAL
                     SEYTKNIIAELKLNIDEYNGLSIDFYIASSEVPALIQADECVRDQTSSELFVIQSKAI
                     SLHNKISSFIKDIYDDNYPIDNFITLKKK"
     gene            complement(968..1789)
                     /locus_tag="ACIYSB_004820"
     CDS             complement(968..1789)
                     /locus_tag="ACIYSB_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431690.1"
                     /translation="MLGESFNQFMVESYLSSTSIGGGLTAVRKCRAHDKGSFYSSFFQ
                     LSIGIERFFKIIFILNHMIENNLEKPDFRTLKKFSHNIAELHKNCSSYGASHLPNLEW
                     ELNWQQNLILEMLSEFADASRYYNLDKIVKGKKEVKDPLAQWNEIINSCFRKHITDSR
                     KQKLERELNLWADKYKAYGYTWNRGLDGAILSQIDEYILSWKIINVSPYIVFEIIDML
                     QPYYYLISKFKDDIDNIEHSKGIREPLVPYLHEIFVFLLVHKKLALSRKTWSFRY"
     gene            complement(1827..2528)
                     /locus_tag="ACIYSB_004821"
     CDS             complement(1827..2528)
                     /locus_tag="ACIYSB_004821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431691.1"
                     /translation="MNIKTINSTLVGIVAVLLFLAVLVLVKVLFAGSRGFEWGNAADL
                     TSALCNIVIASTALCAAFVANNWFVQNKKLKSLSTSHQLAMKFEMQLWEINSRLYNDG
                     IVRASIRKYVQDNKELTDEIKSKVAAEINKKATSDLSELANLYTTRSMLARFDIKLSE
                     RLENLFKDILELRQSYLDNQYIYLLTICKHINCPKHEDVIAATENLESVKRELAAIFQ
                     YELCETNIDTDYSFS"
     gene            complement(2525..3076)
                     /locus_tag="ACIYSB_004822"
     CDS             complement(2525..3076)
                     /locus_tag="ACIYSB_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431692.1"
                     /translation="MEAETITQLIAVGGTLGGALIAAGVTLASQLITKKYENEKLKKQ
                     LLFKILEDELKEKKQTIKPFIQFINSLNLPENFNFYDDGDLLDQIMLYKQDLIKSKAS
                     DFLLDYTLYMTPQIHDAIWAVTNAINDFEELGARFYDSSLSKQENDEILLKNCSGKPT
                     EIWEALMKLKGLLYSEVNIADKL"
     gene            complement(3262..4065)
                     /locus_tag="ACIYSB_004823"
     CDS             complement(3262..4065)
                     /locus_tag="ACIYSB_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin"
                     /protein_id="HGC5431693.1"
                     /translation="MKFVSERIDHVKIANGYCVICGNFGKLKKDHVPPQCASKPSPML
                     QKTISEYFSNTRVPPIKAKSGTTFRTICHNCNSNILGSMDAEIGRVTTEFIEKLNDYF
                     SGKFFYKNYISLKFDADKFLRAMIGHLLAATSVKDCMKPIVDSPFYTPLRNFVLGRNP
                     DIGATHNIYYWFYPFRKQITAQSVTFYNNGHHSICSCLHFFPISFLVTLKEQGTFPIH
                     ATEMTQYDQSLHFNMTTANIDYASFPFVGLKNNQFMMVISGHTCVSYPK"
     gene            4389..5063
                     /locus_tag="ACIYSB_004824"
     CDS             4389..5063
                     /locus_tag="ACIYSB_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431694.1"
                     /translation="MEWKKELRAKFERDELRRLISNVNWIIFKPKIKKQLEILSTQLS
                     GEHVRFNIIDMQDEPDGYGFDCLTFQIADRMTGNMRQEKTTESIKFNHHWQKGAALSI
                     TYSATGAVHIILFPSTSDDSLATHDSLIVHHSYNVKHITPKKIEKAVKNLLHYHRVTG
                     VLHKAALKDLILIRLLKIKMFFIQYNDKENKFRRVTALYIPTLSLIVSLAAAVASILA
                     LVSVKS"
     gene            complement(5342..6118)
                     /locus_tag="ACIYSB_004825"
     CDS             complement(5342..6118)
                     /locus_tag="ACIYSB_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGC5431695.1"
                     /translation="MTVSTYLESIKSNAYQRDITITNSIATIKNRLNGYFAGELVSHF
                     VFGSYSRNTMLPRSYDPSSDVDYMVVFKNVIYQPQTYLNKLRDFVNYYYRTSEIKQSH
                     PTIQLNLNHITFELVPASHNILFGYQIPSNQDFLNRWMNTDPNAFNTDLTNCNKNNNN
                     LIKPLIRIMKYWNANAGYVFESYQLEKDIVSFNYFWCSTLKEYFYSAVEQLSGSYYLA
                     QWKKDKIQHLKNSVMMSKYYEQLNNHFAAENEIKKILPIK"
     gene            complement(6121..6624)
                     /locus_tag="ACIYSB_004826"
     CDS             complement(6121..6624)
                     /locus_tag="ACIYSB_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HGC5431696.1"
                     /translation="MNQKLIQTVISECHRIEEDALYSSKSHYNVSDRWDKLNLWFGVP
                     LAILTALSGIAAIKSTANAVVAISITATILTALNTSLNPSKRAALHKSAANEYNKLKN
                     EVRIFREILTSNFDFTDKTLIEKLTSYSDRRNQLNSSSPNIPRWAYEKTQRDVNQGFT
                     KYNIDKE"
     gene            6789..7310
                     /locus_tag="ACIYSB_004827"
     CDS             6789..7310
                     /locus_tag="ACIYSB_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431697.1"
                     /translation="MNDRYTTNEIAERMGVNDSTVSREWAKRGMPHPKKASKKEIDQW
                     ILINIIQPLRNTDVKEQIEKEKLRKISLEADLVEIELNISTEKVLSTDYVTQTLTGYF
                     AALRGVLRSIPASIYVELFESEDADTLRIKLEERIDEKLRELGSYEYEEQPEEDDEDS
                     EPESEINITPTED"
     gene            7258..7749
                     /locus_tag="ACIYSB_004828"
     CDS             7258..7749
                     /locus_tag="ACIYSB_004828"
                     /inference="COORDINATES: protein motif:HMM:NF017673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGC5431698.1"
                     /translation="MKILNQSLKSILPPPKIKPSEWCEQYTVFPDGPLAQQKTKLFSF
                     QVEPLNATVDPSVQKICLMSSAQLLKTTILQNAMLYRMANKPSHMIFAGSSGSTVKKF
                     RTGKWMDTINACPNLQALITDKSDKNAVNDLHTQQNVDVTNGAIVIHTQRLKSDPGGN
                     LLS"
     gene            7763..>8256
                     /gene="istA"
                     /locus_tag="ACIYSB_004829"
     CDS             7763..>8256
                     /gene="istA"
                     /locus_tag="ACIYSB_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HGC5431699.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     LKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTL"
BASE COUNT         2769 a         1427 c         1409 g         2651 t
ORIGIN      
        1 gtttctttaa tatgcatcct taatgatttt gaatcaagta tgactttttc tattaagtca
       61 tcagtaattc tacaaactta ataggtttgc aagatagggt attaatcatt ttttctttag
      121 agttatgaag ttatcaattg gatagttatc atcatatata tcttttatga aagatgaaat
      181 cttattgtga agtgaaattg cttttgactg tataacgaaa agctcactac tagtttgatc
      241 cctcacacat tcatcggcct gtataagtgc aggaacctca gaacttgcaa tatagaaatc
      301 gatactcagt ccgttatatt catcaatatt taatttaagt tcagcaataa tgttttttgt
      361 gtattcgctt aaagcataat tagttctatc aatagatttt aaggaattaa taacggagta
      421 agaagtgaag aataaatcgg aaacaagagc atctgtttcc ttcataaact catcatgtga
      481 atcttggggt atggtctcgt tttttaaatg attgcgatat tttttggaga atgtcactaa
      541 ttgatgtttt aaaattaagg ttgaagtacg tactttatta agatcttgat aaattgcttt
      601 ctcaataaca ccatatacta catcataatg tttttgcgac aaccattcag gtgcttttag
      661 gtatgcttgg taggctaccc atagtgtacc taatgtacct acagcactac ataatgttcc
      721 aattaaagtt acccactccc atcctttcga accagaaaat aatattttta caccaactaa
      781 tatagcaatt agcagcagta atagtacggt acttagtagt attaaattat attttttaat
      841 attaagcatg ttaatctcat ttcttaaata aaagacatag atgtatatat gtagcattga
      901 tggatatata ttccactaat catgattagt gcctctctga ttaataatgt gatacatgtc
      961 catgtaatta ataacggaaa ctccatgttt ttctactcaa agctaacttc ttatggacaa
     1021 gtagaaatac aaatatctca tggagatatg gaaccagagg ttctcttatc cctttactgt
     1081 gctcaatgtt gtcaatatca tctttaaatt tagaaattag ataataataa ggctgcaaca
     1141 tatcaatgat ttcaaacaca atataaggtg aaacgtttat tattttccag gaaagtatat
     1201 actcatcaat ttggcttaaa atcgcaccat caagacctct attccatgtg taaccgtatg
     1261 ctttgtattt gtcggcccat aaatttaatt ctctttctag tttttgtttt ctagaatcag
     1321 taatgtgttt acggaagcaa gaattaatta tttcgttcca ctgtgctaac ggatctttga
     1381 cttctttttt gccttttaca attttatcaa gattataata tcttgaagca tcagcgaatt
     1441 cagaaagcat ttctaatatg aggttttgct gccagtttaa ttcccactcc aaattgggta
     1501 aatggctggc accatagctt gaacagtttt tatgaagctc cgcaatatta tggctgaatt
     1561 ttttaagtgt acggaagtct ggcttctcaa gattattttc gatcatatga ttgagtatga
     1621 agataatctt aaaaaacctt tctatgccaa ttgataattg aaagaagctg ctatagaagc
     1681 tacctttatc atgtgctcta catttccgca cagcagtaag cccaccacct attgaggtac
     1741 tggacaaata agactctacc atgaattgat taaatgactc cccaagcata agctttcctt
     1801 tttatttaca tcaattaact ataaatttag ctgaacgaat aatcagtatc aatatttgtt
     1861 tcacatagct cgtactgaaa tatggcagct agctctcttt taacactttc aagattttct
     1921 gtagctgcaa ttacgtcctc atgcttgggg caattaatat gtttgcatat ggtcaataga
     1981 tatatatatt gattgtctaa atatgactgc cttaactcta atatatcttt aaataaattt
     2041 tcaagccgtt cgcttaactt aatatcaaac ctggccagca ttgaccttgt cgtatatagg
     2101 ttagcaagtt cagataaatc acttgtggcc tttttattaa tttcagcagc aactttagat
     2161 tttatttcat ctgtcagttc tttattatcc tgaacatatt tcctgatgga agcacgaact
     2221 ataccatcat tatataaacg tgaatttatt tcccataact gcatctcaaa tttcatagcc
     2281 aactgatgtg aggtagaaag ggattttaat tttttattct gaacgaacca attatttgct
     2341 acaaaagcag cacaaagagc tgtagacgca atcacgatat tgcataacgc acttgttaaa
     2401 tcagctgcgt tcccccattc aaaccccctg ctaccagcga atagaacctt aacgagtact
     2461 aagactgcta aaaacagaag tactgcaaca atgccgacaa gagtagagtt tatagttttt
     2521 atattcataa tttatcagct atgttaactt cactatagag caagcctttc aatttcatca
     2581 aagcctccca aatttcagtt ggcttaccgc tacaattttt aagtaatatt tcatcgtttt
     2641 cttgtttact caatgatgaa tcataaaacc gtgctcccaa ttcttcaaaa tcatttatag
     2701 cattagtaac cgcccaaatc gcatcgtgaa tttgtggtgt catgtataat gtataatcga
     2761 gaaggaagtc acttgcttta cttttaatta aatcctgttt ataaagcata atttgatcta
     2821 aaagatcacc atcatcataa aaattaaaat tttctggaag attcaaagaa ttaataaact
     2881 gaatgaaagg tttaatagtt tgcttttttt cttttaattc atcttcgagt attttgaata
     2941 aaagttgttt ttttagtttt tcgttctcat attttttagt gattaattgg cttgccaatg
     3001 ttacacccgc agctattaat gcaccgccta aagtaccacc gactgcaatg agttgtgtaa
     3061 tggtttcagc ttccattttt ttagaaatcc aatatatcaa agtgaaaaat gaactttacc
     3121 ataataacca ttctggcgta aactattagc gacacgcccc actcataaag ggtaggtttg
     3181 tttagttggt gtgtgtgggg cgtgtcgctg aacagttgga ctatgttctt atgtctatat
     3241 ttctcaacct ttagttgaaa atcatttagg ataactaaca caagtatgac cagaaattac
     3301 catcataaat tgattattct ttaaacctac aaagggaaat gaagcataat caatattagc
     3361 cgttgtcata ttaaaatgaa gagattgatc atattgagtc atttcagttg catgaatagg
     3421 aaatgttcct tgctccttca gtgtaaccaa aaaagatatt gggaaaaaat gtaagcaaga
     3481 acatatacta tgatgtccat tattgtagaa tgtaactgac tgagcagtaa tttgttttct
     3541 gaatggataa aaccaatagt aaatattatg agttgcccca atgtctggat tcctgcctaa
     3601 tacaaaattt ctcaatggag tataaaaagg actatcaaca ataggcttca tacaatcttt
     3661 tactgaagtt gcagccagaa ggtgtccaat cattgctctt agaaatttat ccgcatcaaa
     3721 ttttaatgaa atataatttt tgtaaaagaa cttaccacta aaataatcat ttaacttttc
     3781 gatgaattca gttgtaacac ggcctatttc tgcatccatt gatcctaaaa tattcgagtt
     3841 acaattgtga caaatggtac gaaaagttgt cccgcttttt gctttaattg gtggtactcg
     3901 agtattagag aaatactcag aaatcgtttt ttgtaacata ggtgaaggtt tagatgcaca
     3961 ttgcggggga acatggtctt tttttaattt tccaaaatta ccacatatta cacaatatcc
     4021 attagctatt tttacatggt caattctttc agagacaaat ttcattctgc aatctcaaaa
     4081 gttaacatca ccaaaatata gagcattcaa acacaatgtt caatatgatt ttactctaac
     4141 cagtgtgcta ttttttatgc aaacttatac atggacgaat actcaaactg cgataataaa
     4201 ctgaaaatat cgatataaga attgatttta tgagtaatca tcattatagc ctataccatg
     4261 tagcattaac actttaccac caaatagaac atacatatac tattgattat cgacctaggg
     4321 taagtttatc aattatggta cgatagaatc taaataatct atagtagcaa gaggcttagg
     4381 gggagataat ggagtggaag aaagagctta gagcaaagtt tgaaagagac gaacttagaa
     4441 gattaatttc taatgtaaac tggattatat tcaaacctaa aataaaaaag caattagaaa
     4501 tactatcaac ccaattatca ggagagcatg taagatttaa tataattgat atgcaagatg
     4561 aaccagatgg ttatggtttt gattgcctga catttcagat cgctgatcgt atgactggaa
     4621 atatgcggca ggaaaaaact accgaaagca taaagtttaa tcatcattgg caaaaaggag
     4681 ccgcactttc aattacatat agtgcgacgg gggctgtgca tataattctt tttccgtcta
     4741 cttcagatga ttcattagca acacatgatt ccttgattgt ccatcattca tataacgtta
     4801 aacacataac accaaaaaaa atagagaaag cagtaaagaa tttacttcat tatcaccgtg
     4861 ttacaggtgt tttacacaag gccgcattaa aagatttaat tttaatccgc ttactgaaaa
     4921 ttaagatgtt ttttattcag tataacgaca aagagaacaa gtttagaaga gttacagcat
     4981 tatatatacc tacattaagt cttatagttt cgttggctgc cgcagtagca tctattctgg
     5041 cattagtatc tgttaaatca tgatgattat aagccagcaa ctaactggct tataaattag
     5101 aaattggaaa ctatctcttc tcacaaaact gctatcttta gcgataaggg aatgtaatct
     5161 cctgatactg atggtagagg agcgggtcat acaatcttag caatctcatt tagctgtacg
     5221 acggtggaat gtttcaactg tactcaattc ttacaagtaa aaatatcgag aattattttt
     5281 tggattcaag caaagacaat gagctgtacc cccttataat tagtaatata agggggcaag
     5341 attattttat gggtaaaatt ttctttattt cattttcggc ggcaaaatga ttgtttaact
     5401 gctcataata ttttgacatc ataactgaat tttttagatg ctgaatctta tcttttttcc
     5461 attgtgcgag ataataggaa cctgaaagtt gttctacagc agagtaaaaa tattctttaa
     5521 gggtagagca ccaaaaatag ttaaagctaa caatatcttt ttcaagttga tacgactcaa
     5581 agacataacc tgcattggca ttccaatatt tcattatgcg aataagaggc ttaattaaat
     5641 tgttattatt tttattgcaa ttagttaaat cagtattgaa cgcattggga tcggtattca
     5701 tccaacggtt caaaaaatcc tggttggaag ggatttgata accaaataaa atattatgag
     5761 atgcagggac aagctcgaaa gtaatatgat taaggttcaa ttgaatagta gggtggcttt
     5821 gctttatttc agatgtacga tagtaataat ttacaaaatc ccttagcttg tttaggtagg
     5881 tctgaggctg atatattaca tttttgaata caaccatata atcaacatca gaagaaggat
     5941 catagctacg aggaagcatt gtattacgtg aatatgagcc aaatacaaaa tgactaacaa
     6001 gttcaccagc aaaataacca ttcagtctgt tttttatggt tgcaattgaa ttcgttattg
     6061 taatatctct ttgataagca ttgcttttaa tagactcaag ataggtactg actgtcatgt
     6121 ttattccttg tcaatattat acttagtaaa gccttggtta acatctcttt gagttttctc
     6181 ataagcccac cgaggaatat ttggcgaact tgaattaagt tgattgcgtc gatcagagta
     6241 actcgtaagt ttttcaatca aagttttatc agtaaaatca aaattagaag tcaatatttc
     6301 cctaaatatt ctaacttcat tctttaattt attatattca tttgctgctg atttatgaag
     6361 tgcagcacgc ttacttgggt taagagacgt atttaatgca gttagaattg tagcagttat
     6421 ggaaatagct acaacagcat tggctgtact cttgattgcg gcaataccac tcaaagcggt
     6481 taagattgct aatggtacac cgaaccacag attcagctta tcccatctat cagaaacatt
     6541 ataatgagat ttagaagagt acagagcgtc ctcttctata cgatgacact cagaaatgac
     6601 tgtttggatg agcttctgat tcataaacac tccaacaaat ccgattccct aacagtataa
     6661 catggtaggg ttaataaata ctataggtac ttccctctga atatacacaa tcgggaagtt
     6721 tcgccgcgaa ctattttttt tgcatattaa atattttaaa taacacaaac aaaacctgga
     6781 taaacaaatt gaacgataga tatacaacaa acgaaattgc agaacgtatg ggagttaatg
     6841 acagtactgt aagccgtgaa tgggcaaaac gaggaatgcc acacccaaag aaagcaagta
     6901 aaaaagaaat agatcaatgg atactaatta atattatcca accattgcga aatactgatg
     6961 taaaagaaca gattgaaaaa gagaaattac gcaagatatc acttgaagca gatttagttg
     7021 agattgaatt aaatatcagt actgaaaagg tattgagtac tgattatgta actcaaacac
     7081 taactggtta ttttgcagca ctacgcggag tactaagaag tattccggca tctatctatg
     7141 tagaactatt tgaaagtgaa gatgcggata cattgcggat aaaactagaa gaaaggattg
     7201 atgagaaact aagggaatta ggatcatatg agtacgaaga acagccagaa gaagatgatg
     7261 aagattctga accagagtct gaaatcaata ttacccccac cgaagattaa accatctgaa
     7321 tggtgtgaac aatatactgt atttccagat ggaccactgg cacaacaaaa aactaaatta
     7381 ttttcatttc aagtagaacc cctaaacgcc acagtagatc catcagtaca gaagatctgt
     7441 ttaatgagta gtgcacaatt actcaagaca acaatactac aaaatgcaat gctttacagg
     7501 atggcaaata aaccctccca tatgatattt gcgggtagta gtggtagtac tgtgaaaaag
     7561 ttccgtactg gtaaatggat ggatactatt aatgcgtgtc caaaccttca agcattaatc
     7621 actgacaaat cagataagaa tgcagtaaac gatctacaca ctcaacagaa cgttgatgtc
     7681 acgaacggtg caatagtgat ccacacccaa cgcctgaaat cagatccagg gggtaatctg
     7741 ctctcctgat tcaggagagc ttatggtcac ttttgagaca gttatggaaa ttaaaatcct
     7801 gcacaagcag ggaatgagta gccgggcgat tgccagagaa ctggggatct cccgcaatac
     7861 cgttaaacgt tatttgcagg caaaatctga gccgctaaaa tatacgccgc gacctgctgt
     7921 tgcttcactc ctggatgaat accgggatta tattcgtcaa cgcatcgccg atgctcatcc
     7981 ttacaaaatc ccggcaacgg taatcgcccg cgagatcaga gaccagggat atcgtggcgg
     8041 aatgaccatt ctcagggcat tcattcgttc tctctcggtt cctcaggagc aggagcctgc
     8101 cgttcggttc gaaactgaac ccggacgaca gatgcaggtt gactggggca ctatgcgtaa
     8161 tggtcgctca ccgcttcacg tgttcgttgc tgttctcgga tacagccgaa tgctgtacat
     8221 cgaattcact gacaatatgc gttatgacac gctgga
//
LOCUS       DBBGLQ010000156         8227 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.156, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000156 DBBGLQ010000000
VERSION     DBBGLQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.156"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            205..1293
                     /locus_tag="ACIYSB_004830"
     CDS             205..1293
                     /locus_tag="ACIYSB_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016791210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431700.1"
                     /translation="MRNVVSDDKISDFRDLVNSNSSFVYQIYKDKGGKNLFNLVCSAM
                     DWISVSVRHLENAPEFDKNIDSRCMQVYSLISSIDLIFESIKQLHRVFITDKKDPFYG
                     EKKCFKDRLFANEDDNNYFKTIRACFGAHPVNLNQENSKRFASWPFQSHFNTGDLSVH
                     LYSRDVGKEDLTLNLNINELLEFLRIRYEYLDVIADRIETLFVEYQHKLSKEKIETKL
                     DPLEQLYVLRTESEKRLDNDYYNGEIDDLIMIFEAEVTDADLVPLADKYKESLLPLIE
                     EIKTNLQEMNIVDLANDSELRIRSELDKELRYELGKFYTWVHGGRYDPLLEYYFERFN
                     ASTDGKFKFTKTDDIKLTFLKAKLMLTE"
     gene            1702..2880
                     /gene="umuC"
                     /locus_tag="ACIYSB_004831"
                     /pseudo
     CDS             1702..2880
                     /gene="umuC"
                     /locus_tag="ACIYSB_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            complement(2885..3277)
                     /locus_tag="ACIYSB_004832"
     CDS             complement(2885..3277)
                     /locus_tag="ACIYSB_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGC5431701.1"
                     /translation="MDDERKRKKFTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGALQ
                     SAEKMPPSATDSVQEAMNDVRLKMQKLF"
     gene            complement(3282..4253)
                     /gene="parM"
                     /locus_tag="ACIYSB_004833"
     CDS             complement(3282..4253)
                     /gene="parM"
                     /locus_tag="ACIYSB_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HGC5431702.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLVPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            4482..5126
                     /locus_tag="ACIYSB_004834"
     CDS             4482..5126
                     /locus_tag="ACIYSB_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HGC5431703.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQILNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAARAEIEHLMNEVFGPW"
     gene            5120..5395
                     /locus_tag="ACIYSB_004835"
     CDS             5120..5395
                     /locus_tag="ACIYSB_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGC5431704.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(5587..6013)
                     /locus_tag="ACIYSB_004836"
                     /pseudo
     CDS             complement(5587..6013)
                     /locus_tag="ACIYSB_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            6193..7728
                     /gene="cba"
                     /locus_tag="ACIYSB_004837"
     CDS             6193..7728
                     /gene="cba"
                     /locus_tag="ACIYSB_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin B"
                     /protein_id="HGC5431705.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVRSNPEIQEDGVISETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISAVEILDSNSLSQRQEAENNAKDDFRVKKEQENDEKTVLTKTSEVIISVGD
                     KVGEYLGDKYKALSREIAENINNFQGKTIRSYDDAMSSINKLMANPSLKINATDKEAI
                     VNAWKAFNAEDMGNKFAALGKTFKAADYAIKANNIREKSIEGYQTGNWGPLMLEVESW
                     VISGMASAVALSLFSFTLGSALIAFGLSATVVGFVGVVIAGAIGAFIDDKFVDELNHK
                     IIK"
     gene            complement(7746..>8227)
                     /gene="cbi"
                     /locus_tag="ACIYSB_004838"
     CDS             complement(7746..>8227)
                     /gene="cbi"
                     /locus_tag="ACIYSB_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin B immunity protein"
                     /protein_id="HGC5431706.1"
                     /translation="TFSIIGIIPLMAILILRINNPYSQVLYYLYNKVSFLPSITSLYD
                     PVMTTLMSNYNKTAPVMGILVFLCTYKTREIIKPVTRKLVVQSCFWGPVFYAILIYIT
                     LFYNMELTTAGGFFKLLSHNVITLFILYCSIYFTVLTMTYAILLMPLLVIKYFKGRQ"
BASE COUNT         2482 a         1695 c         1826 g         2224 t
ORIGIN      
        1 aggttcagtt cttctatttg tcttcggata atgcctagca atacaactgg ctagagatcc
       61 tcatcgttca aatctaacta agtgacaaaa cccagcgtat cacttcactt caaatcaaaa
      121 aagaagttga agaattgaag aactatcact atcatttttg ccattcttca taatattttc
      181 aattggttag aaggcgatta gagaatgaga aacgttgtta gtgacgataa aatcagtgat
      241 tttcgagact tagttaactc aaactctagc tttgtgtatc aaatatacaa agataaaggt
      301 ggtaagaatc tttttaacct cgtttgctct gctatggatt ggatctctgt ctctgttcgc
      361 cacttagaaa atgcgcctga gtttgacaag aacatagata gtagatgtat gcaggtctac
      421 tctctgatct cttcaataga ccttattttc gagtcaataa aacaactcca tcgagtgttt
      481 ataactgata agaaagatcc gttttatggt gaaaagaaat gtttcaaaga tcgcctcttc
      541 gctaatgaag atgataataa ttattttaaa actatcaggg cgtgtttcgg tgctcacccc
      601 gtaaatctga atcaagaaaa ctcaaagcgt ttcgcaagtt ggccatttca aagtcatttc
      661 aacactggcg atttgagtgt tcatttgtac agtcgagatg tgggtaaaga agacctaacg
      721 ttaaacctca atatcaatga gctgttggaa tttctcagaa ttcgatatga gtatcttgat
      781 gttattgctg ataggattga aacgctgttt gttgagtatc aacacaaact ttcaaaagaa
      841 aaaattgaga ctaaattaga tcctttagaa caactctatg ttcttcgtac tgagtcagaa
      901 aaacgattag ataacgatta ctataacggg gagattgacg acctgattat gatttttgaa
      961 gcggaagtaa ctgacgctga ccttgtacca ttggctgata agtataaaga atccctccta
     1021 cctctgattg aagagattaa gacgaacctt caagaaatga acatcgttga cctagcaaac
     1081 gatagtgagc tgagaataag atctgaactt gataaagagt tacgttatga acttggtaag
     1141 ttctacactt gggttcacgg tggcaggtac gatccgctac ttgaatacta ttttgaaagg
     1201 tttaacgctt ctactgacgg taagttcaag ttcaccaaaa ctgatgacat caaacttacg
     1261 tttcttaaag caaagctcat gctaactgaa tagtgtgaac aaattggctc agcttttatc
     1321 ttttgctgtt cattactttt tgtatatcaa agttacctca acatcactgt ttgaactttg
     1381 ggttgtaact cataatgggg caaaagtgtg tttcaaactt tgttggcaca cttatctaag
     1441 atagccatgt tgataaagca taagtttcga tctttattgt cacaatcacc actcgacgac
     1501 tacgaaccaa tctggcatgc gagactgaga agttttagac attaatgtta tgccacactt
     1561 aattgttgag aattaatgag ccccatatta aaaaattctc aacttattgc ttcatatcgg
     1621 tgagaattac acaaatcagg ctacaggctt tgttcagaaa ggtttttcat aacattttag
     1681 ggtgtaaaac gtgtaacggt gaataacgat ggctgtgtga tcgcgcgcag cccggaggca
     1741 aaagcccttg gcatcagaat ggggcagccc tggtttcagg tgagacagat gcgcctggag
     1801 aagaaaatac atgtattttc cagcaattat gcgctgtacc acagcatgag ccaacgggtt
     1861 atggctgttc tggagtcgct ttctcccgca gttgagccct actcaattga tgaaatgttt
     1921 attgatttgc gggggataaa tcattgcatc tctccggagt tttttggtca tcagctcagg
     1981 gaacaggtaa agagctggac aggactcacc atgggggtgg gcattgcgcc tacaaaaacg
     2041 ctggctaaaa gtgcacagtg ggcaacaaag caatggccac agttttccgg tgtggtcgcg
     2101 ctgacggcag aaaaccgtaa tcggatcttg aagctactgg ggctgcagcc agttggtgag
     2161 gtctggggag taggacgcag actgacggaa aagctgaatg cgctgggtat taacacagca
     2221 ctgcagctgg cgcaggctaa cacggcattc atccggaaaa acttcagcgt cattcttgag
     2281 cgtacggtac gcgaactcaa cggcgagtcc tgaatatccc tggaagaagc accaccggca
     2341 aaacagcaga ttgtctgtag tcgcagtttt ggtgaacgaa tcacagacaa agatgccatg
     2401 caccaggctg ttgttcagta tgcagagcgg gccgcagaga aactacgtgg ggagcgtcag
     2461 tattgccggc aggtgacgac atttgtacgg acatccccct ttgcagtaaa agaaccctgt
     2521 tacagcaatg ccgctgtgga aaagcttcca ttacccacac aggacagccg ggacattatt
     2581 gccgcagcat gcagagcgtt aaaccatgtc tggcgtgaag ggtaccgcta tatgaaggca
     2641 ggagtcatgc tggctgattt cacaccatcg ggtatagcgc agccgggatt atttgatgag
     2701 atccagcccc gtaaaaacag cgaaaagtta atgaaaacac tcgatgaact gaaccagtct
     2761 ggaaaaggga aagtgtggtt tgcggggcga ggaaccgccc ctgaatggca aatgaaacgg
     2821 gaaatgctct cgccttcgta tacaacacaa tggactgaga tccccattgc ttcattctga
     2881 tatatcagaa taatttttgc attttaaggc gcacatcatt cattgcctcc tgcaccgaat
     2941 cagtagcaga aggaggcatt ttttcagcag attgcaaagc accgagttca gtcaacacag
     3001 ctctgatatc ggcctgagaa ggcttccatc ctgtcgtctg actgagtaag gttacaacct
     3061 gatctgctga aaactcctcg ctcaaaatgg cagtcagcaa tacaggcagg cgaggatcaa
     3121 gttggtgtaa cgccattcca gaaataaatg cattccggaa cagctcacca cgctcagaac
     3181 gcggaacgga ctctatcgct tccaaagttt gaaagtctgc agctttttcc ggatgcagat
     3241 aaagtgtgaa ttttttcctt ttccgttcat cgtccacaat attactcctc tttgaaagcc
     3301 gcgatagctt caaccagtgc tgtctgtggc gactccatca tcactacttt ttgcccaaga
     3361 tggtgccatg ccgtttttat cgaatcatag atcaacgggg ccccaccacc gacaacataa
     3421 acacgattca catgatgaaa atcaccgagc tcattgacta cttgctcccc cagactggca
     3481 atagccccct caatggtatt aaggaccaga tcagtttttg tttcgtcatt tataacctgc
     3541 cgaacaaagt ccgggtcatt acgacgttta atcaattcat ctgcaacgag gaaactggta
     3601 tcactggatg ccatgcgcaa tgcactcatt gcagctttcg taactgaaga cacaccaatg
     3661 ccagaattac catgaatggc acttacagag tcaaattgac caacgataac accaacatca
     3721 agcgtagtgc cgccaatatc aactaccagc gatttttcga actgaccaac tttatccatg
     3781 accagacgag aaaacacagc tggcaatgat tcaggcatca catcaacgtg ttcaatggtg
     3841 aaaacatcgc ctttattaag acgaatcggc ctcattaaat tctcaatttt acgctgaatg
     3901 ttgagttcat ttttttgaca ctctttcgtg tagaactcac tgataggcaa agtcacagtc
     3961 aatgatacag gctgaggcac taatccacta ttcagtaaag cgtgatgcac agctaacagg
     4021 ttaacatccg tatattgata ctcaatatga gtcgtaagaa tagattggtt gctaacttca
     4081 tcatacgtgt attttttacc atccagttca tagttaaatg tctggcgaat ccctaacccg
     4141 tcaaccttcc atccatgacg aaatgagttt tgcgacaacg acttacagat tttcccattg
     4201 tcattccatg ccaacttgat tgttgttgaa ccatcatcgc agtatacgtt catctatagc
     4261 cccttaaata ctcaatctga gtaaatcact gtttctggtt gcaaggtaat actcaatttg
     4321 agcactatca tagttatgaa atcacaaaac acaagtaata tttattacat catatgacat
     4381 acctcatatt tcatacttca taaatatgaa tgcatgttga cacatcaggt taccgtgtca
     4441 aaatatcatc atacttcaca catcacattt atgaggttgc aatgattact gtagttggtg
     4501 ggaacaaagg tggttcaggg aaaactacca ttgcatcaaa cctggctatt gctctcgcaa
     4561 ataaaggaag ggaagtctgc ctgttaaacg gagatctaca acgaacagcc gcaaaacatc
     4621 atgcggaacg agaagctgca ggactgttac cagcaataac ccttgttgag aaatttgata
     4681 acctgacaca aacactacag attctgaatg aaaagttcga tgatgtaata gtggatgtcg
     4741 caggaagaaa ctcaaaggaa ttcataacga gtggagttgt cgcacatcaa ataattgcgc
     4801 cactacagtg ttctcaacca gatctggata ctcttacaga actagaacaa cagatagacg
     4861 caatgcgtaa ccttaacccg gaacttaagg tttattgttt acaaagtatg gcaacaacca
     4921 atccggtttt gaggggaaat gagagaaaag aattccttga atatcttgaa gaattcccaa
     4981 caattcaagt cctggattca gttatttgtt tcagaaaagt ctatcgcgat tgcatgagca
     5041 acggcaccgg tgtagtagaa acgaacaata cagcagccag agcagaaatt gaacatttaa
     5101 tgaatgaggt atttggccca tggtgaagaa acccagccag caagcattaa acagagctgc
     5161 agttacggta gaacaagcag aggctctggc tcagcgcctt gcggataaac catacggcgc
     5221 gccggaaaaa cccgaaccag aaaaacaatg tcggacgaca atctctcttg gtgaaagcat
     5281 gcttgtcaca atagaagatc tggcattgag gaataaacgc aatggtaaag atccaaaaaa
     5341 tgtaagtgcc atcgtcagag tagcccttga gcaatactta aaaacactaa cgtaataatc
     5401 aagaagttat tacttatgaa atcacacttg tgaattcaca ctgtttaagc acccttgcat
     5461 acaagagatc acatattgca gtggtctctt cttttataga ataaatcaca attcataatt
     5521 atgatttatg aattatgcgt gtctgttttt cagcattgtg accgcatcgg actcaggcat
     5581 cgaaaactac acccggtgcc gtgcagccac atccagcgca aacaccttcg tgtagacctc
     5641 cgttgagctg atggacttat gccccatcag gctctgcagg accttcagcg gcatacagca
     5701 tgtgcatcgc ataggaatgg cggaacgtat gtggcgtgac cgggacagag aacgtcacac
     5761 catcagcggc agcaaccgct tccccaatcc aggtcctgac ctttctgtcc gtcacttccc
     5821 agatccgcgc tttctctgtt ctgcctgttc gtttattacg ccgttccatg gggattttca
     5881 gtgtggccac catcgtctgc agctggctga cgtaccagga gtccgagagc cgaaccagtc
     5941 ggtgagtctg ctgaccggcg ggcattattc ctgccgtcct gaccagtaat gcttgtgcag
     6001 ttttttgtgg catcaggcga gaatagcgcg tggtgtgagt gcctgttttt tgatcgtttt
     6061 cacaaaaatt gaaatccaca gtcttgacag agaaaacaca gcggcgtaat ttttgatctg
     6121 tacataaaac cagtggttat atgtacagca tttaatttta attgattgtt tttaaagtca
     6181 aagaggtttt ctatgagtga taatgaaggt agtgtaccga cagaaggtat tgattacggg
     6241 gacacaatgg ttgtgtggcc gtcaacagga cgaattccgg gcggtgatgt gaaacccgga
     6301 ggctcatcag gtctcgctcc atccatgcct ccgggatggg gggattacag cccacaaggt
     6361 atcgcacttg tacaaagtgt tctttttcct ggaattattc gccggattat tcttgataag
     6421 gaacttgaag agggagactg gtcgggatgg tctgtcagtg tgcatagccc ctggggaaac
     6481 gagaaagttt ccgctgcacg aacagttctt gaaaatggtt tacgtggtgg tttgccagaa
     6541 ccgtctcgcc cggctgctgt ttcttttgcc cgtctggagc ctgcttccgg aaatgagcaa
     6601 aaaattattc gtcttatggt tacacagcaa ctggagcagg taacggatat ccctgccagc
     6661 cagttaccag cagcgggtaa taatgtaccg gtaaaatatc gtctgacgga ccttatgcag
     6721 aatggtacac aatatatggc tattatcgga ggtattccga tgacagtgcc agtagtggat
     6781 gccgttccag ttccggaccg gagtcgtccg ggaaccaata ttaaagatgt ttacagtgcc
     6841 cctgtatcac caaatctacc ggacctggta ttaagtgtgg gtcagatgaa tactccagtt
     6901 cggtctaatc ccgaaatcca ggaagatggc gttatttctg agacagggaa ttatgttgag
     6961 gctggttata cgatgtccag taataatcat gatgtcattg tccgttttcc tgaaggcagt
     7021 ggagtttctc cgctatatat ttcagccgtg gagattctgg acagtaatag tttaagccag
     7081 cgccaggaag ccgaaaataa cgcaaaggat gacttcagag tcaagaaaga acaagaaaat
     7141 gacgagaaga cggtcctgac aaaaaccagc gaggtcatca ttagtgtcgg tgacaaagtc
     7201 ggggaatatc ttggagataa atacaaggcg ctttcccgtg aaattgcaga gaatataaat
     7261 aattttcagg gaaaaacgat tcgtagttat gatgatgcaa tgtcttccat taataagtta
     7321 atggctaacc ccagccttaa aataaatgca acggacaaag aagccattgt gaatgcgtgg
     7381 aaagcattta atgctgagga tatggggaat aaatttgctg cgttgggtaa gacgttcaaa
     7441 gcagcagatt atgcaataaa ggcaaacaac atcagggaga agagtattga gggttaccag
     7501 actggtaact gggggccatt aatgctggaa gtcgagtcct gggttatcag tgggatggca
     7561 tctgctgtag ctcttagttt gttttctttc acattaggct cggcccttat agcctttggt
     7621 ctttcggcca cagttgttgg ttttgttggc gtagttattg caggtgctat tggtgcattt
     7681 atcgatgata aatttgttga tgagttgaat cacaagatca taaaataatt aaaaggaagg
     7741 cagtgttact gcctcccttt aaaatattta atgacaagca atggcatcag taaaatcgca
     7801 tatgtcatgg ttaaaacagt aaagtaaatg gagcagtata aaataaacag agtgatgaca
     7861 ttatgagata ataatttaaa aaaaccacct gctgttgtta gttccatatt atagaacagt
     7921 gtgatataaa tcagaatggc ataaaaaacg ggcccccaga aacaggattg cacaacaagt
     7981 tttcttgtta ctggctttat gatttctctt gtcttatatg tgcaaagaaa aacgagaata
     8041 cccataactg gtgctgtctt gttgtagttt gacataagtg ttgtcatgac gggatcatac
     8101 aatgatgtaa tagaagggag aaatgatacc ttattatata agtagtacag cacttgagaa
     8161 tatggattat ttattcgaag tattaatata gccattaatg gaataatccc gattatggaa
     8221 aatgtat
//
LOCUS       DBBGLQ010000157         8041 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.157, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000157 DBBGLQ010000000
VERSION     DBBGLQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.157"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            416..634
                     /locus_tag="ACIYSB_004839"
     CDS             416..634
                     /locus_tag="ACIYSB_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGC5431707.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            852..1094
                     /gene="yiiF"
                     /locus_tag="ACIYSB_004840"
     CDS             852..1094
                     /gene="yiiF"
                     /locus_tag="ACIYSB_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HGC5431708.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1424..2353)
                     /gene="fdhE"
                     /locus_tag="ACIYSB_004841"
     CDS             complement(1424..2353)
                     /gene="fdhE"
                     /locus_tag="ACIYSB_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGC5431709.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCDTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2350..2985)
                     /gene="fdoI"
                     /locus_tag="ACIYSB_004842"
     CDS             complement(2350..2985)
                     /gene="fdoI"
                     /locus_tag="ACIYSB_004842"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGC5431710.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2982..3884)
                     /gene="fdoH"
                     /locus_tag="ACIYSB_004843"
     CDS             complement(2982..3884)
                     /gene="fdoH"
                     /locus_tag="ACIYSB_004843"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGC5431711.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3897..6947)
                     /gene="fdnG"
                     /locus_tag="ACIYSB_004844"
     CDS             complement(3897..6947)
                     /gene="fdnG"
                     /locus_tag="ACIYSB_004844"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6360..6362),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGC5431712.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7141..7974
                     /gene="fdhD"
                     /locus_tag="ACIYSB_004845"
     CDS             7141..7974
                     /gene="fdhD"
                     /locus_tag="ACIYSB_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGC5431713.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1881 a         2049 c         2116 g         1995 t
ORIGIN      
        1 caagaaagtt attctgttgt taatttagaa aaacaacttt cccgaactgt taccaacaaa
       61 attataacga tgagcaagta aagaccggtt ggtggcagta ctgaccggtc tgtgatgaaa
      121 gattaatttc accttaaatc ctggtgctgt taggatgctg acgtttttcg gctacaccat
      181 tcagattatg caaataaata tctctgaata taatatattc atagcaccca taattcatcc
      241 tgggcgataa ctaaaccatt tttgagtaat aaatgaaaca actgcaacac tcttattatt
      301 tctggaaaac gcctcgcaaa aacaaaacaa catgcaaaaa tccgcttttt ttaaatgatt
      361 ttccggcgta attttcgcac atacattaat gagtaatata tgtaaggtat tggtaatggc
      421 gatgaatacg gtttttcttc atttatcaga agaggcaatt aaacggctga acaagcttcg
      481 gggctggcgt aaggtttcac gatctgcaat tttacgcgaa gcggtagagc aatatctgga
      541 acgtcagcaa ttcccggtgc gtaaagcaaa gggaggcagg caaaaggggg aggtagttgg
      601 tgttgatgat cagtgtaagg agcataagga atgacaaaaa tttttcattc ttgaatataa
      661 aaaacagatg cccttattct ggtattaata caaggctgtt ttacttgaac ttataataac
      721 tgcaactgtt acatcatatc tggaaaacgc ctcgcaaaaa taaaaaatga tgcgtaaatg
      781 agctttcttt aaagattcgg caagggtact tttctctcca tagattcaaa aatgatattg
      841 gcgagatatt tatgaactct ctggcaggta tagatatggg cagaatttta ctcgatttat
      901 cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca gcgtaatctt cctcgtgcgg
      961 acctattaag ggaagcggta gatcaatacc tgataaatca atcgcaaaca gcaagaacca
     1021 gtgttcctgg catctggcaa gggtgtgagg aagatggtgt cgaatatcag cgtaagctgc
     1081 gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt gctgacaacg tgcgcgttgt
     1141 tcatgccgga tgcggcgtga acgccttatc cggcctacaa agtcgtataa attcaatgta
     1201 ttgcagttta ttggtaggcc tgataagcgc agcgcatcag gcaatcttac gcttgtcatc
     1261 agtctcatgc ccataacctt attgatgtgg tggtgggtaa attgaggcca aaccggcact
     1321 ggcggcatag cttgaggtgg taacgcggaa tacgagagat ggtgtcgtca caccctatga
     1381 gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag agattactct ccttcacccg
     1441 gaaacaggaa cgggttgatg gaactgcggg catagccttc ttgctccatt cgtgcgtcca
     1501 gtaccagaga ggcgaggtca tctgctacgg cttcgatttt tggatctttt tcctgataga
     1561 gaattttcag gtaagtgtcg cagtcatcgc agctttcggc tttaatcgcg gcctgttcgt
     1621 catccagcga ccagtaatgc agtttgccgc tctgctcgca gttgctgcat tttacgcgca
     1681 ctacgtgcca ttcagtttca cacaggttgc agtgcaggta acgtagcccc tgagtggtgc
     1741 caatttgcac catgctggac accggcatag aaccacaaac cgggcaatat tgacgttgct
     1801 cgccgtattc agcgcgggct ttgccgggga tcagattggc catctgcgcc cagtagagcg
     1861 acagtgcagc ccagataaac ggcgctttat cgctgctgac ggacgagaaa tcagaggcaa
     1921 acagcgcgct ggccatatct tccagctcct gagtcgatgc cttctccaga ttctcaatca
     1981 ctgccagcgc cgggccgctc atttcaggtt tcagctcagc aatcagcgcc atcagcagct
     2041 tttgccagtg cttatcacgc ggcagaacgt gaatatccag cgggggcttg ccttgtgcgc
     2101 tggcttcttt aatgcgcgca gtcagatcca tctccagcgg atggtcgtac agcaccactt
     2161 cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc acccagagga ttattttctg
     2221 ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt cttgagccga gggaacaata
     2281 acggcggaat catatccgcc gtacgtttct cgcttgaacc cagctcatct tgcgggatta
     2341 tgcgaatact cattcagctt tcttttccgt tgtcttgcgg acctcacggt accagcgcgg
     2401 gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc accatggcgg taatcgtgcc
     2461 tttcacccaa agggcggcgt agatatgcac catgataacc acaattaacg ccactgcggc
     2521 aaatgaatgc agcattaacg cgaatcggat caccgggatt gagaaagcag gcgcaaaata
     2581 aggacgccag ataatcacgc cgctcaccag cagcagaacc aggaaaataa tcgccgccca
     2641 gaaaacgcat ttctgaccga agttataacg cccggtgtca cctacttcct cgttgacgac
     2701 gatcttacga atattcttcg cccaaaagat atcatcccga ttgattaggt tgtgatgcca
     2761 gtaacggaaa aacatgatga tgaacgaggc aaacataacc acgccgacaa acgggtgcag
     2821 aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac cagttgaagg acgggaacaa
     2881 aaagcccagc ccgctcaccg ccgccaggat gaagcagaag gcggtgatcc agtggttgat
     2941 acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt ttcatttgcg ctcctcgtct
     3001 ttctcttcgt gcagattatt ctcttcctca tccgcacggt tcggaccgac acccacgtag
     3061 tggaagatac tggccgcaaa ggtagccgca aagccaacag ccgcgagcgg tttccagatg
     3121 cctttccaga atttcacggt ttcgctgatt tccgggttct ccggcaagcc atgatacaga
     3181 tttggcttgt cagcatggtg cagcacgtac atgacgtgtg taccaccgac gcctgccgga
     3241 tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct cagcaacgcg ctcgctcgcc
     3301 agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc ccgttgggca ggtcttcacg
     3361 caggctggtt cttgcccaac caccacgcgg tcaacgcaca gcgtacattt gtagacgcgg
     3421 ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc ccgcaatgca ataaccgcag
     3481 ccaatgcact gctcggactg gaagtcgacg ataccgttgg catactgaat aattgcacct
     3541 tccgccgggc acgctttcag gcagcctgga tcggaacagt gcatacagcc gtctttgcgg
     3601 atcagccatt ccagtttgtc gttctgctcc acttccgaga agcgcattac cgtccacgat
     3661 ttggcgctta aatcattggg gttgtcgtac accccaatgt tattgccgac ggtatcgcga
     3721 atgtcgttcc actctgaaca cgccacctga caggctttac agccgataca ggtggtaacg
     3781 tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct gaggcgcggg ggtcagaccg
     3841 ttagtcgcgg aacgacgaat gatatcttga gattgataag ccatatgtcg tctccgttac
     3901 accttttcca cattcacaag gaaggactta aactccggcg tctgcgtgtt cgcatcacca
     3961 acgaatggcg ttaaagtatt ggcaataaag ccttttttag ctacgccttc atagccccag
     4021 tgaatcggaa taccgatggt atcgatatct ttgccgttcg ctttcagcgt gcgaatacgt
     4081 ttggtcacca ccgctttggc tttgatatag ccacggttgg aggagacttt cacggtatcg
     4141 ccctgggcaa tgccaagttt attcgccagc gactccccga tttccacaaa ctgctctggc
     4201 tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt ggaagtgctc ggtcagacga
     4261 taggtggttc cgacatacgg gaacttatcg gctttaccca atgcttcggc gtcgtcttta
     4321 aagatacgtg cagccgggtt cgagataacg tttggatgca gcgggttagt tcccagcggc
     4381 gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg ccatcttatc gagggcaaac
     4441 agacgcccca tgccttcctg ctgcatgata aacggcccga cgccgctacc cggaggcgct
     4501 gcgctgtagt ccggaatatc ccagccggtc cacttagtgc cgtcccattt cagcaactga
     4561 cgcttcggat cccacgggtt accctgcgga tctgcggagg cgcggttata cagaatgcgg
     4621 cggttaagcg gccatgccca tgcccagccc agcgtgttac cgaggccaga cggatcggcg
     4681 ttatcacggc gtgccatctg gttgccttcc ggcgtccagc taccggcgaa aatccagcag
     4741 ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg aactaagttg ttggcctttc
     4801 ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg ccagcgcctt accgttgctc
     4861 tccatcgcca cttcttctga agatggctca tgcggaatgg cgtagttcca tgtcatgttc
     4921 agcacctggt ccgggttcgc gccaccctgt tcggcataca tcttgcgcaa gcgcaggaag
     4981 ataccggaga ggatctcgcc gtcagtcagc gcaatccccg gggcgtccgc acctttccag
     5041 tgccactgta accagcgacc tgagttaacg atagaaccgt tctcttccgc gaagcaggtc
     5101 gatggcagac ggaacacttc ggtctggatc ttcgacgagt caacttcgtt cagctcaccg
     5161 tggttctgcc agaagttaga ggtttcagtg ttcagtgggt cgatggtgac gaggaacttc
     5221 agtttcgaca gacaaccgat cactttgttt ttgttcggga atgaggcaac agggttaaag
     5281 ccctggcaga tatagccatt gaccttgccc tctttcatca tctcgaagta ttgcaggacg
     5341 tcgtagcctt tatcccactt cggcaaccag tcaaagcccc agctattttc cgccgtcgct
     5401 ttatcaccaa agaaggcctt catcatagag acgaagaatt tcgggtagtt gccccagtag
     5461 tttacctggc cttccagcag cggttttggc gtgttggcgg taaggtaggt ttgcagatcg
     5521 gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc tctgcgacag cagccccagg
     5581 tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt taacgccgcc gcctgccatc
     5641 cccatattgc cgagcagcag ctggatcatc gccatcgtac gaatgttttg cgcaccaacg
     5701 gagtgttgcg tccagccgag ggcatacagg aacgaggcag ttttatcgtg agcactggtt
     5761 tctgcgatgt attcgcagac tttcaggaac gcgtcttttg gcgtaccaca gatgttttca
     5821 accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca agttccacac gcagcgcgga
     5881 tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt ccagttcata agtccaggag
     5941 gatttatcgt acttgcgttt ttccgcgtcg tagccggtga acaggccatc ttcaaagccg
     6001 taatcctcac gcacgatcag gctggcgttg gtataggctt cggtgtattc gcggttgaat
     6061 ttttcattgt tcagcaggta cagcaatacg cctgacagga aagcaatgtc agtaccggaa
     6121 cgaatagggg catagtagtc agctaccgcc gccgtacgcg taaagcgagg atcgatcaca
     6181 atcagcttcg cgccgttgtg aattttggct tccatcgccc agcggaaccc gaccgggtga
     6241 gcttcagcgg cattaccgcc catcaccacg acgaggttgg cgttcttgat gtcgacccag
     6301 tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt
     6361 cagacacgcg cctggttgtc gaccgcgagc atacccagcg cgcgggagaa tttttgcgtt
     6421 aaatagccgg tttcgttact cgacgcggaa gcacacagca tcccggtgga gagccagcgg
     6481 ttaacagtca cgccttcggc gttttgcgca atgtagttag catcgcggtc ttctttcatc
     6541 agtttggcga tgcgatcaaa cgcctcttcc caactgattt gctgccattt atcagaacct
     6601 ggcgcacggt attccggaaa cttcagacgg ctttcggagt ggatgaaatc caccaggcca
     6661 gcgcctttcg gacaaagtgc accgcggttg accgggtgat ccggatcgcc ttcgatatgg
     6721 aagatagatg ctttggcgtt ttttgctccg tcaccgaggc tgtacatcaa cagcccacag
     6781 cctacggaac aataggtgca ggtattacgg gtttcgcggg tgcgcagcag tttatactgc
     6841 cgggtttccg cgagtgctac gctgggtgca aaacccagtg ccgctgccgt ggtgcctgcc
     6901 ataccgccag cgcagatctt aaagaactgc cttctgctga cctgcatgga ttgctccttg
     6961 tttcgacatt gtcacgtccc atttacattc gcttgctgcg tgtgcaggga gtgggagtta
     7021 tttttctttg cggaaggggc cgcaaaggtc cagaattggc tcaatttccc tccatccagg
     7081 aagggtttgt aacagaatac cataatgttg gtgtgtgtgt tcttatctgg ttaagagaaa
     7141 gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa atatcacagg tgttcgtcaa
     7201 attgagttat ggcggcgtga tgatttacaa cacccacggt tagatgaggt cgcggaagaa
     7261 gttcccgttg cgctggtcta caacggcatt tcgcatgtgg tgatgatggc gtcgcccaaa
     7321 gaccttgagt actttgcgct cggtttttcg ctttccgagg ggattatcga aagtccgcgc
     7381 gatatcttcg gcatggatgt cgttccttcc tgtaatggtc ttgaagtaca aattgagctt
     7441 tccagccgcc gctttatggg gttgaaggag cgccgccggg cgctggcggg acgtacgggc
     7501 tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa aaccggtgca gccgctaccg
     7561 ttcacccaga cgtttgatct caacaaactg gatgatgcat tacgtcatct caacgatttt
     7621 cagccagtgg ggcaactgac tggttgtact cacgccgctg cctggatgtt gccatctggc
     7681 gaactggtcg gcgggcatga agacgtgggt cgccatgtgg cgctggacaa actgttaggc
     7741 cgccggtcac aagaagggga aagctggcag caaggtgcgg tactggtttc cagtcgtgcc
     7801 agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag agattttgtt tgcggtgtct
     7861 gccgcgacca cgcttgctgt agaagtggct gagcgctgta atctgacact ggtaggtttt
     7921 tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc gtttgagcaa ttaattaaaa
     7981 taagtcctgc gaaatatatt aaattgcagg acttattcat ttcgtgaatt ttattatttt
     8041 a
//
LOCUS       DBBGLQ010000158         7995 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.158, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000158 DBBGLQ010000000
VERSION     DBBGLQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.158"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..76
                     /locus_tag="ACIYSB_004846"
     CDS             <1..76
                     /locus_tag="ACIYSB_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGC5431714.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..820
                     /gene="hchA"
                     /locus_tag="ACIYSB_004847"
                     /pseudo
     CDS             92..820
                     /gene="hchA"
                     /locus_tag="ACIYSB_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
     gene            complement(928..2286)
                     /gene="hprS"
                     /locus_tag="ACIYSB_004848"
     CDS             complement(928..2286)
                     /gene="hprS"
                     /locus_tag="ACIYSB_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGC5431715.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(2286..2957)
                     /gene="hprR"
                     /locus_tag="ACIYSB_004849"
     CDS             complement(2286..2957)
                     /gene="hprR"
                     /locus_tag="ACIYSB_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGC5431716.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHTLNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3090..3503
                     /gene="hiuH"
                     /locus_tag="ACIYSB_004850"
     CDS             3090..3503
                     /gene="hiuH"
                     /locus_tag="ACIYSB_004850"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGC5431717.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3612..4616
                     /gene="msrP"
                     /locus_tag="ACIYSB_004851"
     CDS             3612..4616
                     /gene="msrP"
                     /locus_tag="ACIYSB_004851"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGC5431718.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            4617..5252
                     /gene="msrQ"
                     /locus_tag="ACIYSB_004852"
     CDS             4617..5252
                     /gene="msrQ"
                     /locus_tag="ACIYSB_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGC5431719.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            5509..6159
                     /gene="zinT"
                     /locus_tag="ACIYSB_004853"
     CDS             5509..6159
                     /gene="zinT"
                     /locus_tag="ACIYSB_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HGC5431720.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            7252..7581
                     /locus_tag="ACIYSB_004854"
     CDS             7252..7581
                     /locus_tag="ACIYSB_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGC5431721.1"
                     /translation="MSNPNPCITCGACCAFFRVSFYWAEADDAGGTIPARLTEQVSPL
                     HRCMIGTNQKNPRCIALAGTPGKEASCTIYENRSSTCREFAMSGENGIENEACNRARS
                     RYGLPPI"
BASE COUNT         2253 a         1825 c         1676 g         2241 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cccaaaaccg tatcgcggta aacataaaat
      121 tctggtgatc gccgcggacg aacgttattt gccgaccgat aacggaaaac tgttctcgac
      181 cggtaaccat ccgattgaaa cgttgctgcc gttgtatcat ctccatgctg caggtttcga
      241 attcgaagtg gcgaccattt ccggtctgat gaccaagttt gaatactggg ctatgccgca
      301 caaagatgaa aaagtaatgc cattctttga gcagcataaa tcgttgttcc gcaatccgaa
      361 aaaactcgcg gatgttgtcg tcagcctcaa cgcagacagc gaatatgcag caatctttgt
      421 tcctggtggt catggcgcac ttattggttt accagaaagc caggacgtgg ctgctgcttt
      481 gcagtgggca atcaaaaatg accgttttgt tatctccctt tgccacggcc cagcggcttt
      541 tctggcgctt cgccacggcg ataacccact gaatggttat tccatttgcg cattcccaga
      601 cgccgcagac aaacaaacgc cagagatagg ctatatgccg ggtcatctca cctggtactt
      661 cggcgaagag ctgaagaaaa tgggcatgaa cattattaat gacgacatca ccgggcgagt
      721 acataaggac cgtaaagttc tcaccggcga cagtcctttc gcagcgaatg cgttgggtaa
      781 actggcggcg caggaaatgc tggcagctta tgcgggttaa tccctgttac gtatgaatgt
      841 aagcgcataa tcaatcgcag ccacgttaat actcacgacg tggctgcgtt ttttcatggt
      901 tgatttatgg cgtactgaag ccctatgtta atttctttgc ggtaacgtaa ttcggaacac
      961 attatgctta ttaaaatagt gatacgtagc acttccccca tgcaattcgg caatcgcttt
     1021 gactaaagaa agaccaagtc cctgaccttc ggaatgacgc gaattatctc cccgccaaaa
     1081 tctacggaag agtttttcag gctcattaat tttcgttcca ggactggcga tatcaatatt
     1141 aagatagccg ttggtatcaa gaaaactggt tatatgaatg cgcgattttt ctggtgaata
     1201 tctaatggca ttaacaataa gattcgataa cattcgttgc agtaaaattt tatccgcaaa
     1261 gatttgctga ttgcactcga ccttaaagca aatctctttc tcgtctgaaa ggtattcaag
     1321 ataatccaac aaattttcga cttccttatt gagaaaaagt gagtccagtt tcaccaaaac
     1381 attgttttta tctgcccggg caagaaacag tatgttctct gttaaccgcg aaatattttc
     1441 cagctcttca atgttaccgg caattgtttt ttgatattca gcgatacttc tggtttgact
     1501 gagcgtaacc tgattctgac ccagtaatgc attaatgggc gttctaagtt catgagcgag
     1561 atcgtcagca aactgactta gacgctcaaa atctttgact aaagcatgat gcattttatt
     1621 caacgcctgc ccaagaggtt ttagttctct cggtaatgcg ctaacctcaa caggctcccg
     1681 gctatcgtta taattcagcg cttccgttac accactcaac tttttgatct ctcgtaatcc
     1741 cgttctgatt aacagcggac ttaatactga gcaaagtaca atggcgacaa tgcaaattat
     1801 aatgctatta attttatact gttcaagcat gttatgtctg gctgaagcca atttagccac
     1861 agtaaccgtt aatgatggcg aaacttcatc aatattaatt cgtaaagcat ctatctctgt
     1921 atcattaata atgcttctgt aaatgccagc tgcgctgatt gtctcagtag caggtatgtt
     1981 atttaacatg tcatcactga cattcgtccg gttaacaatt ttgttgatgc tatcaccatg
     2041 aatgatcaag atatcctgac taacatccat catccggtta aagtacacag gtaacgtatc
     2101 tggatttacc ccatcaatta acaactgctt gatctgcgct gtccggttaa tgagtgttgt
     2161 atcatcgcgc catttcaact cacttgccag gccattatag agagtccata caattccagc
     2221 gccagcaaca gacagcagca atataaaaag caaggttaaa cggacggtta tagataatct
     2281 tttcattatt tttttaccgc tacgaatgaa tagcccatcc cccggattgt ggcgattagc
     2341 ttttcaggaa aaggatcatc aacttttgcg cggagcctgc gaatggcgac gtccaccgta
     2401 ttggtatcac tatcaaagtt gattccccaa atttcactcg caataaccgt tctggggata
     2461 atctcgccag ctctggaggc cagtagccaa agtaactgaa actccttgcg cgtcagtgta
     2521 atactgatat tgtccctgct cacactttga ctaacagagt ccattcttaa gccactgatt
     2581 tctaatgttg aattcagagt gtgatgttgc cttaattgtg cccgaaccct tgccagcaac
     2641 tcagaaaatg aaaaaggttt taccagataa tcatttgccc cactgtccag ccctctgact
     2701 ctgtcatcga cagaatctct tgcagtaagg caaataaccg gagtttgctt tgctgttctt
     2761 aacgtttgta agatctgcca gccatccata cccggaagca taatatccag aatgatcaat
     2821 gcataatcat ccttaagcgc aagataaagc ccatctctgc catcagaaac ggcatcaatg
     2881 acataacccg cttcggaaag cccctgcgtt acccactcct gggtcctttg attatcttca
     2941 ataagtaaaa tcttcatgaa aataatatgc cataaatcat atgttataga aacagcctgg
     3001 ttcattacaa aattgtaatg ctgctgtaag gttaccgtgg acgctttttc gctatcctca
     3061 aaactcattc acatgacaag gatataaaca tgttaaagcg ttatttagta ctctccgtag
     3121 taacggctgc attttcatta ccttctttgg tctatgctgc acaacaaaac attcttagtg
     3181 tgcacatttt gaaccagcaa acaggaaaac ccgctgccga cgtgacagtc actcttgaaa
     3241 agaaagcgga caacggctgg ttacaactta ataccgccaa aacagataag gatggacgaa
     3301 ttaaggcact gtggcccgag caaactgcaa ctacgggcga ttaccgtgtc gtatttaaaa
     3361 ccggggacta tttcaagaaa caaaatcttg aaagtttctt ccctgagatc cccgttgaat
     3421 ttcatattaa taaagtgaac gagcattatc atgtgccttt attacttagc caatatgggt
     3481 attcaaccta tcgtggcagt taatgcataa atatgtaaaa gcagatctct gctactgtta
     3541 gggaggacac tccctgacag attaacagta aacggctctt gctggctaaa gacaaaaaag
     3601 tgtgatggct tatgaaaaag aatcaatttt taaaagaatc agatgttacg gccgagtcgg
     3661 tattctttat gacgcgtcgg caggtgttaa aagcactggg catcagcgcg gctgcacttt
     3721 ctttgcctca cgctgcgcat gccgatctgc ttagctggtt taaagggaat gatcgcccac
     3781 ccgcccccgc cggaaaaccg ttggagttca gcaagcctgc cgcctggcaa aatgacctgc
     3841 cactgacgcc agcagataaa gtctccggtt ataacaactt ctatgaattc gggctggata
     3901 aagccgatcc cgccgctaat gctggtagcc tgaaaaccga tccatggaca ctgaaaatca
     3961 gcggcgaagt ggcaaaacca ttgaccctcg atcacgatga tttaacccgt cgctttccgc
     4021 tggaagagcg tatttatcgt atgcgctgcg tggaagcatg gtcgatggtg gtgccgtgga
     4081 ttggttttcc gctgcacaaa ttgctggcgc ttgccgaacc caccagcaat gcgaagtatg
     4141 tcgctttcga aacaatttat gcaccggaac aaatgccagg ccagcaggac cgctttatcg
     4201 gtggcgggct gaaatatcct tatgtcgaag gattgcgtct cgacgaagca atgcatccgc
     4261 tcacactgat gaccgtgggt gtttatggca aggcgttacc gccacaaaat ggcgcgccgg
     4321 tgcgactgat tgtgccgtgg aaatatggct ttaaagggat taaatcgata gtcagtatta
     4381 agctgacccg cgagcgtccg ccaaccacct ggaatctggc agcgcctgac gaatacggtt
     4441 tttacgccaa cgttaatccg catgttgatc acccgcgctg gtcacaggct accgaacgat
     4501 ttattggttc aggcggcatc ctcgatgtac agcgccagcc aacgctactg tttaatggtt
     4561 acgccgagca ggtggcatcg ctgtatcgtg gcctggattt gcgggagaat ttctaaatgc
     4621 gtctgacagc aaaacaggtg acatggctga aagtttgcct gcatcttgcc ggattgttgc
     4681 cgtttctctg gctggtctgg gcgattaatc acggtggatt gggtgccgat ccggtgaaag
     4741 atattcagca ttttactggt cgcactgcac tgaaattttt gctggcgacc ttgttaatca
     4801 cacctctggc acgctacgca aaacagccgt tattgatacg cactcgccgc ctgttaggct
     4861 tatggtgctt tgcctgggcg acactgcatt taaccagtta cgcattgctg gagctgggcg
     4921 tgaataatct ggcgttatta ggtaaggagt taattacccg gccttattta acgttaggta
     4981 ttatcagctg ggtaattttg cttgcgttag cgttcacttc aacccaggcg atgcagcgaa
     5041 aactcggtaa acactggcaa cagttgcata acttcgtcta tcttgtcgcg atcctggccc
     5101 caatacatta tttgtggtca gtgaagatta tttcaccgca gccactcatc tacgctgggc
     5161 tggcggtact gcttttagcc ttacggtata agaagttgct ttcccttttt aaccgtttac
     5221 gcaagcaggt tcacaacaaa ttatctgtat aacttcatat tgccgacaaa gtacgcgtta
     5281 tccctgccgg atgcggcgcg agcgccctat ccggcctacg cagatcacat caagttatgt
     5341 ttcgtttgtc atcaacctca aatcatcgac ataatttatt ttatttcaat aagatagata
     5401 agcacacctg agagaactat tctcttgtct cctaagagtt gttgcatttt gttatatgtt
     5461 acaatataac attacacatc atatacatta actctggagg aaactgtttt ggcgattcgt
     5521 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5581 ggtcatcact cacacggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5641 tttgatgatg ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5701 gtttatcctt tactgcaaag tggcaaactt gaccccgtct ttcagaagaa agcggatgca
     5761 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tgtgacagac
     5821 atcgagatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5881 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     5941 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6001 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6061 tcccaacagt ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6121 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6181 tatttaacag aacagtgcct ggtagtaaca acaataattt gccaagacaa ataagtaaga
     6241 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaaa
     6301 tgcgaaaaag gcgaacaata aatacacaac tcagccatac agactggcag cacaaatgtt
     6361 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6421 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6481 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6541 ttgactcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6601 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6661 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagt gctgaatgtc
     6721 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6781 gtttaggaaa agcaaggcgg gaagtcagga gataagtcat tgataaagtg gcggagagag
     6841 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6901 gtcgaaaaat caaagagtta aaaatttttc ttggaataaa tgtcttaaaa tcgtacacga
     6961 aaaatcaata atttacataa cattaaagaa gaatattcca agccttcttg aggccaccat
     7021 ctgttgtcag gtgtcattca gacgtgattt gccccagatt gggaaagtac gcttttttct
     7081 cagcttattc cgttttgcct gtgttgaata attccactgc agttatataa aatcggtaac
     7141 ggctggaaat cattcaatac tcgcactatc gaaagttaac cagccagctg cagcacgttc
     7201 ttgcatacga catgctgcgg ttttctttat aatttatagc tgtggtgaat tatgagcaat
     7261 ccaaatccat gcataacgtg tggtgcctgt tgtgcatttt tccgcgtctc tttttactgg
     7321 gccgaagccg acgatgctgg cggcaccatt ccagccagac ttactgaaca agtatcgcct
     7381 ttgcaccgat gcatgatcgg tacaaaccag aaaaacccca gatgtattgc ccttgcagga
     7441 acaccaggta aagaagcgag ctgtaccatc tacgaaaacc gttcatcaac gtgccgggaa
     7501 tttgccatgt ctggtgaaaa tggtattgaa aacgaagcat gtaaccgtgc acgttcccgg
     7561 tatggacttc caccgattta actttttcct tccttgtatt tacacattac acattttgcg
     7621 ctacaaaaat ttcacgtggc acgcaccacg tgatccctag aaaagcctct tgttacgaat
     7681 ttcgtaaacc gcacgtctgt ctggtcgtgc ggatttttta tccgtcaact tcacatgaaa
     7741 cccgccagat cactgtttcc ggattcagac atcaggcata ttatcatcca caatgtcgtg
     7801 aacaagatca cctttttgca attcctttat tgatcgaaag cagttaatca ctgaaattaa
     7861 ttaccggagt gaaagcatta ccgcataata aatatagttc cttgcccccc tctgacagca
     7921 ttttcagagg ggttttttta tcagtttcac catccaaccc tgtacgccag aatggtttca
     7981 ctgtcactca gttct
//
LOCUS       DBBGLQ010000159         7862 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.159, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000159 DBBGLQ010000000
VERSION     DBBGLQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.159"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(76..474)
                     /locus_tag="ACIYSB_004855"
     CDS             complement(76..474)
                     /locus_tag="ACIYSB_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431722.1"
                     /translation="MYETTKLSIHEVSNVEWNIVTCKIVSCNAARTDAGHKSTITGYM
                     VMAYRGLRVRFIWKARQLTPFREPSMPLEVEQDYLSLPEIDGATLVDSDGRKADFEVS
                     LKVAQAVINNSRGKWIAKVKHVVRLSRWWC"
     gene            complement(474..1049)
                     /locus_tag="ACIYSB_004856"
     CDS             complement(474..1049)
                     /locus_tag="ACIYSB_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431723.1"
                     /translation="MLTANDNAYAEADLILSDFIGQRERWTQKREELYASLVRKGVNI
                     ETAQSGDMTVVSVGLHGVSVSAANHEPYVALSESMARLIKFLKQTEANNVFIAKKNIN
                     FSAAMFWMMKGLDARRASWPKGSYLSMFRGSIGSKEKLFEFLPEEAFDIVEGCDVMVM
                     PRLVMMNGDLQAQTDWFATGVDIIATDWEAF"
     gene            complement(1503..1578)
                     /locus_tag="ACIYSB_004857"
     tRNA            complement(1503..1578)
                     /locus_tag="ACIYSB_004857"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1543..1545),aa:Ile,seq:cat)
     gene            complement(1703..1867)
                     /locus_tag="ACIYSB_004858"
     CDS             complement(1703..1867)
                     /locus_tag="ACIYSB_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGC5431724.1"
                     /translation="MASKARIAIAIGFLLLSVLVDFTSTILSVLSDGALVAVAVILVW
                     PIFKTASKVQ"
     gene            complement(1867..2301)
                     /locus_tag="ACIYSB_004859"
     CDS             complement(1867..2301)
                     /locus_tag="ACIYSB_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HGC5431725.1"
                     /translation="MFGKLFGKKVASAKVELKKVENRDLMEAIIGGCLLVSAADGEIE
                     KEETAKLDQLVRSNPRLSHFGNEITATITRFTEQLEAGFRVGRMNILREIEDIKNDPK
                     EAEEVFVNMLTIAEADGEIEPAEHKVLEEVGRRLGLRVEDYL"
     gene            complement(2569..2655)
                     /locus_tag="ACIYSB_004860"
     tRNA            complement(2569..2655)
                     /locus_tag="ACIYSB_004860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2621..2623),aa:Ser,seq:tga)
     gene            complement(2844..2919)
                     /locus_tag="ACIYSB_004861"
     tRNA            complement(2844..2919)
                     /locus_tag="ACIYSB_004861"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2884..2886),aa:Thr,seq:tgt)
     gene            complement(2924..3000)
                     /locus_tag="ACIYSB_004862"
     tRNA            complement(2924..3000)
                     /locus_tag="ACIYSB_004862"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2964..2966),aa:Arg,seq:tct)
     gene            complement(4248..5456)
                     /locus_tag="ACIYSB_004863"
     CDS             complement(4248..5456)
                     /locus_tag="ACIYSB_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431726.1"
                     /translation="MSQQTDTSNLEIISAAIETLRTQIALIQKRNPGDDLSRRLHESV
                     IATTDNLVAEINQLLEEGTVDYNKLVDQFEEYQQAVNDGLLRFSQVTGVSATVESLGD
                     AVNQFAASMRSEIGNLEARLEQANTLRKSAEADLSRYKKDYPASLSKRLDVAEKDNRA
                     LKRERRELKERLTELNQQCIKYQGEGVTLRKKLAAAQNIIETLKRECSQLGHDLNRAC
                     GMGQRPETFPLMYDGVDAIAYIHEYPHGLVAETGQRGEALLTANYHQQIRTNRLLTMD
                     VIPSVWGTPLYYRLPGFETDWNTDIDECLADKIMAYLETDFPRLHRRIMDSKDAPIDE
                     LKMRPETLEAIKQTAFDTVFSVACIPSSFHESIPFMQGDRRQEIIDACRVWANEWDKK
                     NGGVEDLYGK"
     gene            complement(5571..5870)
                     /locus_tag="ACIYSB_004864"
     CDS             complement(5571..5870)
                     /locus_tag="ACIYSB_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431727.1"
                     /translation="MSKKYHVNLAFADDAGRTRSITLNTARKVVTAPLIREALRELEM
                     GENSTLLSVSWLGKMSEKEYVDGVTPMTAMRLLSLLQWAIVPVCIVYFIYQAMTQ"
     gene            complement(6082..6540)
                     /locus_tag="ACIYSB_004865"
     CDS             complement(6082..6540)
                     /locus_tag="ACIYSB_004865"
                     /inference="COORDINATES: protein motif:HMM:NF020354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HGC5431728.1"
                     /translation="MLEKIARKRRRRSEYSETSPHAAALRELEKNPDLIKRNHEHYEQ
                     VRVLDWLYRNMRYVYEHTHATPNGGLRGIRTAIKMVAEGQKKGYPDLSIDLACGGYHG
                     MRIEMKHGRNRLTPEQLVWMTRLTEAGYYCFEARSAAEAIKAITEYVCLD"
     gene            complement(6715..6975)
                     /locus_tag="ACIYSB_004866"
     CDS             complement(6715..6975)
                     /locus_tag="ACIYSB_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431729.1"
                     /translation="MRKNRRFTLEDLKEYSISKGYVLEFHRYKKVFTLRKAENPASWS
                     WVYFPHTEDKLVELVDDLTYEGWLIAIDKTITEISEPDKINI"
     gene            complement(6978..7412)
                     /locus_tag="ACIYSB_004867"
     CDS             complement(6978..7412)
                     /locus_tag="ACIYSB_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="olxA"
                     /protein_id="HGC5431730.1"
                     /translation="MTTLIDTVKPTESYLEEILPQALNGRSEDEFLTVYLAGMVERLK
                     KDPQLYHLYGPWWPALKTILLERGDTSFGQVVDSDVAEIYRMSRPALTVLAGHLYSSD
                     RLENDAVYNPVHALEVAAYADDTEPYVYTSYDESIEKYRIMG"
     gene            complement(7449..>7862)
                     /locus_tag="ACIYSB_004868"
     CDS             complement(7449..>7862)
                     /locus_tag="ACIYSB_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431731.1"
                     /translation="DYVSLKNGGATESEIAERLEQAESKREEQAAVAEARAEEAANDD
                     YIIVVNDKPIRAKARVRGRWWSVSEDVGAVYLIADQPGSSTIRNAKDAIKKIGGRFWN
                     FEANPVADVNFDRPAWMVSTRYSVDELRKIIADAA"
BASE COUNT         1932 a         2088 c         1703 g         2139 t
ORIGIN      
        1 gggatgataa tgagaatgtt atttctgtat agactggcaa aaagttggtt atattagaaa
       61 attaattcca ttaatctaac accaccagcg agataagcga acgacgtgtt ttaccttagc
      121 gatccactta ccacgtgagt tattaattac ggcctgcgcc acctttaagc tgacttcaaa
      181 atcagcctta cgaccatcag aatccaccag cgttgcaccg tctatttccg gcaggcttaa
      241 ataatcctgt tctacctcta atggcattga cggctctctg aatggtgtca attgccgagc
      301 tttccagata aagcgaactc tcaatcccct ataagccatt accatgtagc ctgttatcgt
      361 gctcttatgg cctgcatcag tacgcgcagc attacacgat acaatcttac aggtgacgat
      421 attccactcc acgttggaga cttcgtgaat gcttaatttt gttgtctcgt acatcagaat
      481 gcctcccagt cagttgcgat aatatccacg ccagttgcga accagtctgt ctgcgcctgt
      541 aaatccccat tcatcattac caggcgaggc ataaccatca catcgcaccc ttccacaata
      601 tcgaatgcct cttccggcaa gaattcgaag agcttttctt tgctgccaat gctgccacgg
      661 aacatcgaca aatagctccc tttaggccat gatgcccggc gggcatccag ccccttcatc
      721 atccagaaca tggcggcaga aaaattgatg ttctttttgg cgatgaaaac attattggct
      781 tctgtttgct tcagaaactt aattagcctt gccatcgact cggataacgc tacgtatggc
      841 tcatgattgg ctgcggacac gctcacacca tgtaatccaa cactgactac cgtcatatcg
      901 ccgctttgag cggtttcaat gttgacacct ttgcgaacta acgaggcata aagttcctct
      961 cgcttttgtg tccagcgttc ccgctgaccg atgaagtcac tcaatatgag atctgcttct
     1021 gcatacgcgt tatcgttcgc cgttaacata acgtctcctt tttacacgcg cgaccatccc
     1081 tcggttaaac cgatagagat gtcgaaactt cgtattaatt aagggttaca gcctgagcgg
     1141 ctatatgatg aattgaaagg agttgtggcg gtggtgcctc cacctgccag gttagccact
     1201 cctggcgacg tcacttatca gaacgtagtg aatgaaaatg gcttcgtcac gagcgcatag
     1261 ccgcaattac cacaacggaa acggcgctca cgctaattaa acgccttttc ctgttgtgcg
     1321 ccgtactctt ccggctgtca caccgaatcg ccaggatggt gagtcctcgg tccgacgata
     1381 tgaacggggc ttgcacattc cggctacctg gtttgttgcc tgagccaggg gaaaaggtaa
     1441 cccctttaac gtcaccagac cgctaacgac gcatgtgcca gacgccgtgt tacaaccaaa
     1501 tatggtggcc cctaccggat ttgaaccggt gaccgtgcga ttatgagtcg cctgctctaa
     1561 ccactgagct aaggggccgg attactgttt actaagtgct tcaatggcgc taacaatgcc
     1621 gactacaact atgccaacaa taatatggca acaataatta tgagaacaat tggatgcttg
     1681 tcagcaaaat cccagaagcc catcactgaa ccttcgaagc tgttttaaat ataggccata
     1741 ccaatattac agctactgcc accaacgccc cgtccgataa aactgacagg attgtgctgg
     1801 tgaaatccac cagcacggac agcaagagaa aaccaatggc gattgcgata cgtgccttgc
     1861 ttgccattac agataatctt ccacacgaag acctaaacga cggcctactt cttccagcac
     1921 tttgtgttct gctggctcga tttcaccgtc cgcttctgca attgtcagca tgttaacgaa
     1981 tacttcttcc gcttcttttg gatcgttttt gatatcttca atttcgcgaa ggatattcat
     2041 gcgaccaaca cggaagccag cttccagttg ctcggtaaaa cgggtaattg ttgcagtaat
     2101 ttcgttacca aaatggctaa gacgcggatt agaacggaca agctgatcaa gtttcgctgt
     2161 ttcttctttt tcgatttcac catcagcggc agacaccaac aaacagccac cgataatggc
     2221 ctccatcaga tcgcgattct caactttttt cagctctact tttgcagaag cgactttctt
     2281 gccgaacaat ttaccgaaca ttggttatcc ctcaataaaa gtgacatatt tattagattg
     2341 cggtgcctgg tgcctccagg tgacgtcaac cagttaacaa ttaacgccgg gatgtttgac
     2401 ttaaccacta aagaggattg ttttaactgt tccgcgtgcg cttagccgca ttcaccgcaa
     2461 tggtaagagc atttggctga ctgggcggcg atgacgcctg tacgcatttg gtgatccggt
     2521 tctgcttccg gcattcgctt aattagccaa atactcttaa cgttgcgctg gcggagagta
     2581 atggaatcga accatcatcg cttgcgcaat gggacgattt tcaagaccgc ttgagcacca
     2641 tgctccctac tctccagtga ttgtgatggt cggtgctgaa ctccgacaca gggttgtagc
     2701 aagccccgca aagcgcgcac tactgtagtt gcggcacatc agcctgtgca ttcatcacaa
     2761 cggtaagggt acttcgtagg gattcgaacc ctctgccaag cacggcgatc tccgacgtcg
     2821 caaaataccc ttacctgttg tgttggtgcc gattaacggg atcgaaccgc tgaccattcg
     2881 cttacaaagc gactgctcta ccaactgagc taaatcggca atttggcgat ggtgggtgga
     2941 ttcgaaccac cgaccgacag cttagaaggc tgctgctcta tcctactgag ctacaccacc
     3001 attgtgccgg gtacgtctcc ggcgagggct tccacctccg tatgcttttc ggcgcaccgc
     3061 gccctgactg caattcggta acaggggatg cacaaccctg gcttccagcg tgattagcgc
     3121 cttcagcatg acgggatata cccgtaaatt tgttggacag taccaaaagt gctgttaagc
     3181 actggcggtt acgcggaaag gacgtaacag gtaagggcgc tgaccagaaa gacctgaccc
     3241 ttctcattca tctggttaat cacaccagcg cccttgcctg ttatgcctcc ccgttcccta
     3301 atacacagac ggggacactc tgcggtcgat tttttgacgg gggacgactc ataccccgtg
     3361 gcgtcaggct tcttaggccg ctaccatcat cagatcatcg tttgcattta ctttagtggt
     3421 cggtttctaa accgccgcaa agtcgccaac catgacgaaa accctaaaaa agcccacccg
     3481 aagatgggca aatacgctac tacatatcac acaagaaaga agccgactgc ctgagctgga
     3541 ttcacttcct aagacccgca gaaaggaatc acaagtcggc ttctttcttg atgcggcact
     3601 ctctccgccc gtcaccgctc tgtctcggtt gtcgcgtttg ccacgccagc cgtaacgagg
     3661 tttaaagtct tttcacgttt tcatcactcg actgccgtct atggctgttc gttgcggcgg
     3721 gggtgcctcc ccctggggat atccccggcc ttaccccatt ctttcaagac acaatgcaag
     3781 gccgcatccg cataggtgca ttaccgcaac attaaggaga cttagggcaa aaggtaatcg
     3841 ccacaaaaaa atccctatgc ctccttaatg ttgaggatgt gcattgatga tgattcgatg
     3901 cggcgagtcc cgttactctc tgccggacca tcatcaatga acatcttaaa aagaccttcc
     3961 gtggctcaac atgtcttgtt gtttactccg taaagtgctc tctctgaaac cactttacgc
     4021 actaaacctg ctgaactttg gcttgctcgg tgccaaagcg agtccctgta tgcatgtaat
     4081 tttctaacac tttaatttgt tatttgctgg tgggcgaaca acaaccaata agtgactatc
     4141 aagcccagca agcgaaatgt ggcaacgctc caccagcaag gaaaattcta agttaagtta
     4201 ctctcacaat caatatgttc gtttggtgaa attagatttc taacacatta tttcccataa
     4261 agatcttcta cgcccccatt cttcttgtcc cattcgttag cccacacccg gcaggcatca
     4321 ataatttctt gacgacggtc gccctgcata aaggggatgc tttcatgaaa actacttggg
     4381 atacaggcca cgctgaatac ggtatcaaac gcagtctgtt tgatagcttc caacgtctct
     4441 gggcgcattt tcaattcatc gatcggcgca tccttagagt ccataatccg gcgatgcagg
     4501 cgcgggaaat cagtctccag gtacgccata attttatctg ccaggcattc gtctatatcg
     4561 gtattccagt cagtttcgaa gccgggcagg cgatagtaaa gcggcgttcc ccatactgat
     4621 gggatcacat ccatcgtcag caggcggttg gttctaattt gttgatggta attagccgtc
     4681 aacagagctt caccacgctg accagtctcc gcaaccagac catgcgggta ctcatggata
     4741 taggcaatag catccacccc gtcatacatg agcgggaatg tctctggcct ttgtcccata
     4801 ccacaagcgc ggttcagatc atggccaagc tgtgaacact ctcgtttcag cgtttcaata
     4861 atgttctgcg ctgctgctaa ctttttacgc agtgtgacac cttcgccttg atacttaatg
     4921 cactgctgat tcagctctgt taaacgctct tttagttccc gacgctctct tttcagcgca
     4981 cggttatcct tctccgcaac atccagtcgt tttgacaggc tggctggata gtcttttttg
     5041 taacgactca aatcggcttc tgcggatttg cgcagtgtgt ttgcctgttc aagacgcgct
     5101 tccagattgc cgatctcact acgcatactc gcggcaaatt gattaacagc atcgccaaga
     5161 ctttcaactg tcgctgatac gcctgtgact tgtgaaaaac ggagcaagcc atcattaaca
     5221 gcttgttgat attcctcaaa ctgatccacc agcttgttgt aatcaaccgt cccttcctca
     5281 agtagctggt tgatctccgc gaccaggtta tcagtcgtgg caatgacgct ctcatgtaac
     5341 cgtctggaca ggtcatcccc cggattccgc ttttgaataa gggcgatctg tgtccgcagc
     5401 gtttcaatcg cggctgagat gatttccaga ttagaagtat cagtttgttg actcattgca
     5461 gttcccattg tttttgcaaa attagttttc taacatattt tattagtatg agaacaagat
     5521 cgaaagatgt gataaacaaa aaacccgcta atcggcgggt ttttgagcgg ttattgtgtc
     5581 attgcctggt agataaaata cacgatgcat accggtacga tagcccattg cagcaatgaa
     5641 agcagcctca tggctgtcat tggagtaact ccgtctacgt attctttctc tgacattttc
     5701 cctaaccaac taaccgacaa aagggtcgag ttctccccca tttccagctc cctgagggct
     5761 tcccggatta aaggcgctgt taccaccttg cgggcagtgt tgagcgtaat gctccgcgtc
     5821 ctcccggcat catcggcaaa cgccaggttc acatgatatt ttttgctcaa tgtacaaact
     5881 cccaatcatc agcgcctgtg ctttccgggg taatgtgaac cttatcacca gtgacaatat
     5941 tccgagcctc tattgttcca ttgctgtgtt tcaacaactc aacacacaat ggttgctgcc
     6001 aggaacgacg gcgagcaaca acgttagcac cagcagggat aagttcagcc acgttaagta
     6061 aacgcaaaac gatttctccg tttaatcaag acaaacatat tccgtgatag cttttatggc
     6121 ttcagcggca ctgcgcgcct caaagcagta gtaacccgct tcggtgaggc gagtcatcca
     6181 gacgagttgt tcgggagtca ggcgatttct cccatgtttc atctcaatgc gcattccgtg
     6241 gtatccaccg caagcaaggt ctatagaaag atcgggatat cccttctttt gcccttctgc
     6301 caccatcttt attgccgtcc ttatgcctcg cagaccgccg tttggagtgg cgtgtgtatg
     6361 ctcatacaca tagcgcatat tgcgatacaa ccaatccagg acgcgtacct gctcgtaatg
     6421 ctcatggttc ctttttatta gatctgggtt tttctccagt tctctgagag ctgcggcatg
     6481 tggggatgtt tcagaatatt cactgcgacg ccttcttttt cgcgctattt tttctaacac
     6541 acccattgat agtaaataag cgaaataata acaactgcgt tagatttgta aaacaagaga
     6601 aatactactc aaggctgggc tatatgcaac aggtcggatt tgcttttaga gtaaagagtg
     6661 accttaatca acaaggaggg taaatcgcat ggcatttacc caacttgtta ttttttaaat
     6721 gtttatttta tccggttcgg atatttctgt gattgtcttg tcgattgcga tcaaccaccc
     6781 ttcataggtt aaatcgtcaa caagctctac cagtttgtct tctgtatgag ggaagtatac
     6841 ccagctccag ctcgccgggt tttctgcttt tcgcagcgta aaaaccttct tgtaacggtg
     6901 gaactcaagg acatacccct tcgatatgga atattccttt aggtcttcga gcgtaaaccg
     6961 acggttcttt cgcatgacta ccccataatt cgatatttct ctatagactc atcgtagctt
     7021 gtgtatacat atggctctgt atcatcggca taagccgcga cttccagagc atgaacggga
     7081 ttgtataccg cgtcattttc caggcgatcg ctcgaataca gatgtccagc taaaaccgta
     7141 agcgccgggc gactcattct gtagatctct gccacatcgc tatctacaac ctgtccaaat
     7201 gatgtgtcgc cgcgctccag caatatcgtt ttaagcgcag gccaccacgg accataaagg
     7261 tgataaagct gtggatcttt ctttagtctt tcaaccatcc cagcgagata tactgtcagg
     7321 aactcgtctt cactcctgcc attgagcgcc tgcggcaaaa tttcttcaag gtaagattct
     7381 gtcggtttaa ctgtatcaat taatgtcgtc attcaaattc ggccccggct ggggccgctc
     7441 cttatctgtt aagccgcatc ggcgattatt ttacgtagtt catctaccga gtaacgcgta
     7501 gataccatcc atgccggacg gtcaaagttc acatcagcaa ccgggtttgc ttcaaaattc
     7561 cagaaacgcc cgccaatctt cttaatcgcg tccttggcgt tccgtattgt cgaggaaccg
     7621 ggctgatcgg caatgagata aacggcaccc acatcttcac tgacactcca ccagcgcccg
     7681 cgtacacggg ctttagctcg aatcggctta tcattaacca caatgatata atcatcgttc
     7741 gcagcttctt cagctctggc ttcagccact gccgcctgct cttctctttt gctttcagcc
     7801 tgctcaagtc tttctgcgat ctctgattcg gtggctccgc cgtttttcag cgatacatag
     7861 tc
//
LOCUS       DBBGLQ010000160         7556 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.160, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000160 DBBGLQ010000000
VERSION     DBBGLQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.160"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..352)
                     /gene="dndE"
                     /locus_tag="ACIYSB_004869"
     CDS             complement(<1..352)
                     /gene="dndE"
                     /locus_tag="ACIYSB_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndE"
                     /protein_id="HGC5431732.1"
                     /translation="MLPNRMVLNRQTEEQLKRLKGYTGITPNVAARLAFFRSVESEFR
                     YSSERDNRKLDGSLVLDKITWLGETLQTTELVLKMLYPQLAQKDMVKAWAAHVEDGIA
                     ALRNHKSLLNFIKSI"
     gene            complement(352..2352)
                     /gene="dndD"
                     /locus_tag="ACIYSB_004870"
     CDS             complement(352..2352)
                     /gene="dndD"
                     /locus_tag="ACIYSB_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndD"
                     /protein_id="HGC5431733.1"
                     /translation="MLIKQLVLHNFRVFNGTHTIDLAPRKRPHEVNPRPIVLFGGLNG
                     AGKTSILSAIRLALYGRLAFGLATQQQDYIEHLSSLIHKGAYYIEQPEEAAVELTFTY
                     NKGGQESEFTVTRTWKKGKKDRLSLQKDGQPLSELDYDQCQGFLNELIPHGIADLFFF
                     DGEKIAELAEDESGNILRTAVRRLLGLDLISKLRNDLMIFVKRQQSSQLAETQQQQIE
                     VLEKQSRDLACQTEALLEKADFAKSRIEFLSKDIIRYEGLLNAQGGAFAQTKAQEKQK
                     VETLLKDKERLEKALRQECDGSLPYALAPNILSELLEKIADETQIKQAQSFEKELRLF
                     LAQLRNDIALRSRTTSKIAAEAITDNLNEYMATKPKGDLLFDISEREAGMLQQSIEQD
                     SKKAWQRFELYRIQLAEIEQQLEQAAANIARAPEDEQLMDLFEKLRDLDKQRENQRQK
                     YLSLLEDAKRVKQQQLDCVRQVQKLHDAARSQHGFSSAFKNAQETINLLDRYSDVLTQ
                     ARVKTLSANFEVAYRKLARKEDMQLSAHINPETFDVELIDEKGSVINRKLLSAGEKQI
                     YAIAILEALAKTSGRDLPVIIDTPLGRLDSQHRDKLINHYFPFASHQVVLLSTDTEVD
                     ERYFVDQLRDDISHAYEIVFNTHTKSSALKPGYFWELTKEAI"
     gene            complement(2342..3976)
                     /gene="dndC"
                     /locus_tag="ACIYSB_004871"
     CDS             complement(2342..3976)
                     /gene="dndC"
                     /locus_tag="ACIYSB_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HGC5431734.1"
                     /translation="MSKLVQAYDLAEYTDFINQEQFSGRPLAEYISEVQRIYCADKRP
                     WVIGYSGGKDSSAVITLVYLALLGLPPEMRNKDVFVVSSDTLVETPVVVDLIKKTMLQ
                     IEAGAKRNGLPITQHAVTPKTNETFWVNLLGKGYPAPTRSFRWCTERMKINPVSDFIK
                     EKVSQFDEVIVVLGSRSSESASRAQVIAKHKIDGSRLARHTTLANAFIYTPIDTWDVE
                     DVWKLLRGAFRYAPEYIDEWESPWGGNNRPLWTLYMDSSAQGECPLVIDESTPSCGNS
                     RFGCWTCTVVTKDKAMESLIKNGEEWMSPLLKYRDLLAFTTDPVNKDTYRNYKRRTGK
                     VSYQYAKDGEDRSAERKHVPGPYWLKYRQQWLKDLLEIERDLNAQGHRITLITESELH
                     AIRQEWLKDPNEPDWYDTLPGIYREVYQRDLDWVVDDQSRFDASDADLLVQIAQGFDV
                     VPEMVMKLIELEVSMEGLSRRQGIFDKLGTILKQDWGSLEEIQQQQAALQKRNDRDIH
                     QEEITRLEAELQVLQRRIVDASESSVLVGEENERAH"
     gene            complement(3973..5058)
                     /gene="dndB"
                     /locus_tag="ACIYSB_004872"
     CDS             complement(3973..5058)
                     /gene="dndB"
                     /locus_tag="ACIYSB_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndB"
                     /protein_id="HGC5431735.1"
                     /translation="MASVDADYCYSFPAIRGIQAGRPFYIATCPMRIIPKIFSFDEND
                     VPPELRAQRTLNKSRIPEMVRYLLENPKDYVFSALTASIAVDVQFDEFPGTNNLGTLC
                     VPMDAQILINDGQHRRKAIEDALDARPELGQDNIPVLFFVDEGLKRSQQMFADLNKYA
                     IRPSPSLASLYDHRDASSNLARYLAMSVEPFVGMTELEKSNISQRSNKLFTLSSIKQA
                     TRALLGKDPKKSDFEECTQIATRYWQAIYNVMPDWQLAAKKEVSPVQLRQDYVHAHGI
                     GLQALGQLGYTLLTNYPEQWPDKIAALKVFNWRRNNPDLIKRAMQHGKLSKTSTAIQL
                     TCNALKNALSIPLTPEEQELEAQMVVS"
     gene            5521..5694
                     /locus_tag="ACIYSB_004873"
     CDS             5521..5694
                     /locus_tag="ACIYSB_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HGC5431736.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKTNTAHNVKARERIPQLNDK
                     QSPALLKKHRGGI"
     gene            5691..6035
                     /locus_tag="ACIYSB_004874"
     CDS             5691..6035
                     /locus_tag="ACIYSB_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGC5431737.1"
                     /translation="MTKRQRKNRKRINRLVELWPELFNREKPQPLKVEIPDDLIQDIA
                     IRELAFGAGALRAAVASYVRSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLM
                     MLNDRRKARKYR"
     gene            complement(6054..6602)
                     /locus_tag="ACIYSB_004875"
     CDS             complement(6054..6602)
                     /locus_tag="ACIYSB_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431738.1"
                     /translation="MQITEALISEPGDIRRFIQQAVDHWPHLLAFYFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHGGATIRCLLLFSQELFCH
                     PRASVTVDEECSQVVDLLQQSWQVISAGGQCRVEKRFQVARGDTSGQYVALKTVALSL
                     GLPVVTAIIYRPVQRCTLITAQ"
     gene            complement(6690..6848)
                     /locus_tag="ACIYSB_004876"
     CDS             complement(6690..6848)
                     /locus_tag="ACIYSB_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431739.1"
                     /translation="MKRVAMSALVLRKSALSLVLATLPFIFLLTLSGQPADCSSFLTD
                     SSFHSHCL"
     gene            complement(6845..7081)
                     /locus_tag="ACIYSB_004877"
     CDS             complement(6845..7081)
                     /locus_tag="ACIYSB_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5431740.1"
                     /translation="MLTSMTGHDCVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(7150..>7556)
                     /locus_tag="ACIYSB_004878"
     CDS             complement(7150..>7556)
                     /locus_tag="ACIYSB_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431741.1"
                     /translation="TWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQ
                     EYFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSRSPDCEDILKDLIWA
                     FSDFARDRRGVCDPEARCLAGNPGYPGSAGPF"
BASE COUNT         1950 a         1896 c         1679 g         2031 t
ORIGIN      
        1 ctattgattt aataaagttt aatagactct tatgattcct taacgccgca atcccatcct
       61 ccacatgcgc agcccatgct ttaaccatat ccttctgagc taactgcgga taaagcatct
      121 tcaataccaa ttccgtagtc tgtaatgtct cccccagcca cgtaatctta tccaacacca
      181 gcgaaccatc tagtttccga ttatcccgtt ctgatgaata gcgaaactca ctctccaccg
      241 agcggaaaaa tgccaatcgg gcagcgacat taggtgtgat ccctgtatac cctttcaatc
      301 ttttaagctg ctcttcagtc tgacgattca gcaccattcg attcgggagc atcagatagc
      361 ctccttagtt aattcccaga aataaccggg cttcaacgct gatgatttag tatgcgtgtt
      421 aaatactatc tcgtaagcat gagaaatatc atcacgtaat tggtcaacaa aataacgttc
      481 atccacttcc gtatcggtag acaatagcac cacttgatgg ctggcaaatg ggaaataatg
      541 gttaattagt ttatcccgat gttgtgaatc cagacgtcct aatggcgtat caataatcac
      601 cggcaaatca cgaccagaag tttttgctag cgcctcaaga atagcaatgg cataaatctg
      661 cttttcaccg gcagatagta acttacgatt aatgacagat cccttctcat caattaactc
      721 aacgtcaaat gtctctgggt taatatgagc actcagttgc atatcttctt tacgagccag
      781 tttgcgatag gcaacttcaa agttagcaga tagcgtcttt actctcgcct gagttaatac
      841 gtcgctatac cgatcgagta aattaattgt ttcctgcgca tttttgaagg cgctactaaa
      901 gccatgctga ctgcgggccg catcatgcag tttttgcacc tgacggacac aatccagttg
      961 ctgttgctta acccgtttag cgtcttccag caatgaaaga tatttttgcc gctgattttc
     1021 acgctgctta tcaagatcgc gcagcttttc aaaaagatcc attaactgct catcttccgg
     1081 agcgcgggca atattggcag cggcttgctc tagttgctgt tcaatttctg ctaactgaat
     1141 acggtaaagc tcgaagcgct gccatgcctt tttactgtcc tgctctattg attgctggag
     1201 catgccggct tcacgttcag aaatatcaaa caacaaatcg cccttcggct tagttgccat
     1261 atattcgttc agattatcag taatcgcctc ggctgcaatt ttactggtag tacgagagcg
     1321 taatgcaata tcgtttctga gctgggccaa aaatagcctt aactcttttt caaagctttg
     1381 agcttgtttg atctgcgttt catcagcaat tttttccagc aactcggata aaatattggg
     1441 agctagtgca taaggtaatg atccatcaca ttcctgacgc agtgcttttt ccaggcgctc
     1501 cttatctttt agcagtgttt caactttctg cttctcctgc gcttttgtct gcgcaaatgc
     1561 accgccctgc gcattcaaca agccttcata gcgaataatg tctttagaca gaaactcgat
     1621 tctagatttg gcaaaatcag ctttttccag caatgcttct gtctgacaag ctaagtctct
     1681 actttgtttt tccagcactt caatctgctg ttgctgagtt tccgctaact ggctggattg
     1741 ttggcgctta acgaaaatca ttaagtcatt acgcagtttg gatatcagat ccaggcccaa
     1801 taaacggcgt acagcggtgc gcaaaatatt gccagattca tcttctgcca gttccgcaat
     1861 tttttcgcca tcaaaaaaga acaaatcagc aataccatga ggaattaatt cattaagaaa
     1921 cccctggcac tgatcataat ccagttcact gagtggttgg ccatcctttt gtaaagaaag
     1981 gcgatctttc ttgccctttt tccaagttcg cgtaacagta aactctgatt cctgcccacc
     2041 cttattatag gtaaaggtca gttcaacagc cgcctcttcc ggttgctcaa tatagtacgc
     2101 acctttgtga atcaacgaac taagatgctc aatataatcc tgttgctgag tcgcgagccc
     2161 aaatgccagc cgcccataca atgccagccg gatagccgac agaatagacg ttttacctgc
     2221 accatttaaa ccgccaaaca gcacaatcgg acgagggttt acttcatgtg gtcgctttct
     2281 tggagccaga tcaatcgtgt gagtaccgtt aaatacccga aaattatgta ataccagttg
     2341 tttaatgagc acgttcgttt tcctcgccga ccaatacaga ggattcgctg gcatcgacaa
     2401 ttctgcgttg taaaacctga agttcagctt ccagcctggt gatttcttcc tggtgaatat
     2461 cccggtcgtt acgtttttgt agcgctgcct gctgttgttg aatctcttcg agactgcccc
     2521 aatcctgttt taaaatggtg ccaagtttgt caaaaatccc ctgacggcga cttaatcctt
     2581 ccattgaaac ttccagctca atcaatttca tcaccatctc tggcacaaca tcaaacccct
     2641 gagctatctg caccagcaaa tcggcatcgc tggcgtcaaa acgtgattgg tcgtcaacga
     2701 cccaatccag atcccgctgg tacacctcac ggtagatacc tggtagtgtg tcgtaccagt
     2761 ctggttcatt aggatccttc aaccattctt ggcggatagc gtgcagttcc gactcggtga
     2821 ttaacgtaat cctgtggccc tgggcgttta aatcacgctc aatttccagt aagtctttta
     2881 accattgctg gcgatatttg agccagtagg ggcctggcac atgcttacgc tctgcactgc
     2941 gatcttcacc gtctttagcg tactgatagc tcaccttacc ggtacgacgt ttatagttgc
     3001 gataggtgtc tttattcacc ggatcggtgg taaacgccag cagatcacga tatttcagca
     3061 atggcgacat ccactcttcg ccatttttga tcagactttc catcgcttta tctttggtta
     3121 ctacggtaca ggtccagcat ccaaagcgag agttcccaca agagggggta ctttcgtcta
     3181 tcactaaggg acactcaccc tgtgcggaag agtccatata cagcgtccat agaggacgat
     3241 tgtttccacc ccacgggctt tcccattcgt cgatatattc aggagcatag cggaacgcgc
     3301 cgcgcagcag tttccagacg tcttctacat cccaggtatc aataggggta tagataaacg
     3361 catttgccag tgtggtatgc cgggccaagc gtgagccatc aattttgtgc tttgcgatga
     3421 cctgcgcacg agaggcactc tcactactac gtgaacctag gacaacaatt acttcatcaa
     3481 actggctgac cttttcttta ataaagtcac tcaccggatt gattttcatg cgttcggtac
     3541 accagcgaaa actgcgggtt ggggccggat aacctttccc cagtaaattg acccagaagg
     3601 tttcattagt tttaggcgtt accgcatgtt gagtgatcgg caggccatta cgctttgcgc
     3661 ctgcctcaat ttgcaacatc gttttcttaa ttaaatctac taccactggt gtttccacca
     3721 gcgtatcgga tgacactaca aaaacatctt tgttgcgcat ttccgggggc aggccaagca
     3781 gggccagata caccagcgtg atcacggcgg aagaatcttt tccaccgcta tagccaatta
     3841 cccacggacg cttatccgca caataaattc tctgaacttc agaaatatat tcggccagag
     3901 ggcgtccaga aaactgctcc tgattaataa aatctgtgta ttcggccaaa tcgtaggcct
     3961 gaactaattt actcatgaga caaccatttg ggcttcaagc tcctgttctt caggagtaag
     4021 cggaattgag agtgcgtttt ttagcgcatt gcaggtgagt tggattgccg tactggtttt
     4081 gctcaacttt ccatgctgca tcgcacgttt gataagatcc ggattatttc tgcgccagtt
     4141 aaacactttg agtgcagcaa ttttatcagg ccactgctct gggtaatttg tcagcagtgt
     4201 ataccctagt tgtccgagtg cctgcaaacc aataccgtga gcgtgaacat agtcctgacg
     4261 tagttgaacc ggtgaaactt ccttttttgc ggctaattgc cagtccggca ttacattata
     4321 aatagcttgc caatagcgag ttgctatctg ggtacattct tcaaaatcgc tctttttcgg
     4381 atcttttccc agcaacgcac gggtagcttg cttgatgctg cttaacgtaa ataatttatt
     4441 tgaccgctga ctgatattgg atttttccag ctcagtcatt ccgacaaacg gctctacgga
     4501 cattgccaga taacgcgcca aatttgaact ggcatcccga tgatcataca acgatgccaa
     4561 cgacggactg gggcgaatag catatttatt cagatcggca aacatctgtt ggctgcgttt
     4621 taatccttca tcgacaaaaa acagcacagg tatgttatcc tgccccagct ccgggcgagc
     4681 gtctaaagcg tcttcaatcg ctttacgacg atgctgaccg tcgttgatca agatttgcgc
     4741 gtccattggt acacataacg tccctagatt attcgtgccg ggaaactcat caaactggac
     4801 gtcaacggca atggatgctg ttagtgcaga aaatacataa tctttaggat tttcaagaag
     4861 ataacgaacc atttcaggga tgcgagattt attgagggtg cgttgcgcgc gcaactccgg
     4921 aggaacatca ttttcatcaa agctaaaaat tttggggatg atacgcatcg gacatgtggc
     4981 aatgtagaag gggcggcctg cctgaatgcc acgtatagcc ggaaacgagt agcagtagtc
     5041 tgcatcaaca ctagccatgc tgtaatccgt aaaatattta atgaattaag ttacgtgata
     5101 ttttacgtaa ccctagatag aaattacagc attttacgta atgaattgtg tactatctac
     5161 agatgtactc gccaattagc tctattcaat ggtcttgctg gttgttgcaa gtggtgttca
     5221 ttatcatttg tcaagtaata ttttatcata cgcatgtcgt cattaccatc atttaggctt
     5281 tcagcatcag agggcacatg gctgtatgta ttggtacggc tggtccgact gtagcggtca
     5341 ccatagtcat atcgttcaca accagtacag tctgataacg aatacgctca ctacgcacag
     5401 cactgaacaa taccggcaac agcatgtctt acatgacatt cgctgtgcca tccaccggaa
     5461 aacattttca gataccggat gaggtaccac ttccattgac agcacgaggt aacaatactg
     5521 atggtaacgc tgaccattaa ccgtaaaccg cagggtattt tcagtaagtc accggcaacg
     5581 ccgctgcagg ataaaacgaa cactgcacac aacgtgaagg ccagggaacg gataccacag
     5641 ttgaacgata aacagagccc tgctctgctg aaaaaacacc gtggcggcat atgacaaaac
     5701 gccagcgtaa aaaccgtaag cgtattaatc gtcttgtgga actctggcct gagttattca
     5761 accgggaaaa accccaaccg cttaaagttg agatacctga cgacctgata caggatatcg
     5821 ccatcagaga actggcgttt ggcgcaggtg cattacgcgc ggcagtagcg tcttatgtgc
     5881 ggtctccgcg ctattaccgt gcgttaatgg ctggcggcgc ccgttatgac ctgaaggggc
     5941 agccgtgcgg ggaagtgact ccacaggaac agaaagaggc agagacccgg ctgatgatgc
     6001 tgaatgaccg acgaaaagcc agaaaatatc gttgactgca caactgcgct gattcactga
     6061 gctgtaatca atgtacagcg ctgtaccgga cgatagataa tggcggtcac aaccggtaac
     6121 cccagagaca atgcgactgt ttttaacgca acatactggc cagatgtatc accccgggcg
     6181 acctgaaaac gcttttccac ccggcattgt ccgccagcac ttatcacctg ccagctctgt
     6241 tgcagtaaat ccaccacttg cgaacattct tcatcaactg tcacactggc tcgcggatgg
     6301 cagaaaagct cctggctgaa cagcaacagg catcgtattg ttgctcctcc atgttgttcc
     6361 cgcaaccagc gtaataccac cggcgaggat ggactggccg tgtgattacg ctcagtaata
     6421 cgttcgtgca cctgtcggta aaaggaagta cagaatgcat gaatctgttg cccgttgata
     6481 tttccttctg ccgaatggag tgtgaagtaa aatgccagca aatgcggcca gtggtccacc
     6541 gcctgctgaa taaaacgccg gatgtctccc ggctctgaaa ttaatgcttc ggttatttgc
     6601 acgaaatgtt ccttttcaaa ccctgttgaa ttttgttcag ccggtgcaga cacacgggaa
     6661 ggtgcgcatc aacaccggtg taatgccagt tataaacaat gtgaatggaa tgacgaatca
     6721 gtcaggaaag agctacaatc tgctggctga ccggagaggg tcagcaggaa gatgaacggt
     6781 aatgtcgcca gcaccaggga cagggctgat ttccgcaata ccagcgccga cattgcaaca
     6841 cgtttcatgc tgctgctcct cgtgattcct caattcgctg ctgcatccac tgctccactt
     6901 cacttttgta ccagcggctg ctgcggccca gcttgatggg tttcgggaaa tggccttcac
     6961 tgatcagctt gtaaaaccat ttatctgtca taccggtgaa actggtgatg tagttcatgt
     7021 cgatcagggg atcgtcggca cgcagcaaca cgcagtcgtg gcctgtcatt gaggtcaaca
     7081 tggtcagaat atcctctgtg agatttatga aggttgtgcg aatgcgggga aaggtgctga
     7141 gccgtattct cagaatggtc ctgcagatcc gggatagccg ggattaccgg cgaggcaacg
     7201 ggcttccgga tcgcacacac cacgacgatc gcgggcgaaa tcactgaacg cccagatcaa
     7261 atctttgagg atgtcttcgc agtccggtga acgactgtcc agcaccacat cccgggttat
     7321 ctgtttctgg tacggtgccg cttcccccgg tgacgcgaag cccagtacgt tgctccaggc
     7381 atccagaatc agccggtcca gttcggggcc gtcgaaatac tcctgtctga cgcacagcac
     7441 cacgtggtat tccggctttt ccggatgacg ggtcaggagc cagcaaaggt cgcgtccggg
     7501 tttcgcattc atcagcgcac tgacggtttc gataaaatcc ataccaaacc aggtct
//
LOCUS       DBBGLQ010000161         7443 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.161, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000161 DBBGLQ010000000
VERSION     DBBGLQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.161"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..101)
                     /locus_tag="ACIYSB_004879"
     CDS             complement(<1..101)
                     /locus_tag="ACIYSB_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431742.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAG"
     gene            complement(315..1955)
                     /gene="pgm"
                     /locus_tag="ACIYSB_004880"
     CDS             complement(315..1955)
                     /gene="pgm"
                     /locus_tag="ACIYSB_004880"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGC5431743.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEEAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(1981..2526)
                     /gene="seqA"
                     /locus_tag="ACIYSB_004881"
     CDS             complement(1981..2526)
                     /gene="seqA"
                     /locus_tag="ACIYSB_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGC5431744.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            2711..3475
                     /gene="ybfF"
                     /locus_tag="ACIYSB_004882"
     CDS             2711..3475
                     /gene="ybfF"
                     /locus_tag="ACIYSB_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGC5431745.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGTLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     SDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            3615..3908
                     /gene="ybfE"
                     /locus_tag="ACIYSB_004883"
     CDS             3615..3908
                     /gene="ybfE"
                     /locus_tag="ACIYSB_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGC5431746.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            4048..4578
                     /gene="fldA"
                     /locus_tag="ACIYSB_004884"
     CDS             4048..4578
                     /gene="fldA"
                     /locus_tag="ACIYSB_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGC5431747.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            4788..4874
                     /gene="uof"
                     /locus_tag="ACIYSB_004885"
     CDS             4788..4874
                     /gene="uof"
                     /locus_tag="ACIYSB_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGC5431748.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            4867..5313
                     /gene="fur"
                     /locus_tag="ACIYSB_004886"
     CDS             4867..5313
                     /gene="fur"
                     /locus_tag="ACIYSB_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGC5431749.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5397..5723)
                     /gene="chiQ"
                     /locus_tag="ACIYSB_004887"
     CDS             complement(5397..5723)
                     /gene="chiQ"
                     /locus_tag="ACIYSB_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGC5431750.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(5773..7179)
                     /gene="chiP"
                     /locus_tag="ACIYSB_004888"
     CDS             complement(5773..7179)
                     /gene="chiP"
                     /locus_tag="ACIYSB_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HGC5431751.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSFGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         1805 a         1888 c         1853 g         1897 t
ORIGIN      
        1 tcgccagcta atgatgattg ttcaggttgg gcgcatgcag tcagcagtaa tgtggcggta
       61 attagtgaca ctatgacgac cagactcctg ttggtcttca ttgggatttc cttgagctac
      121 tcagtttgtt gacaaagtgt tcgttgtttt tgccggatgc ggcgtgaacg ccttatccgg
      181 cctacaaaat cgcttaaatt caatatattg caccatcctc gtaggcctga taagcgtagc
      241 gcatcaggca attctgtgtt tgtcatacgt aaaaaagggc gatcttgcga ccgccctttt
      301 tttattaaat gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc
      361 tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcttct
      421 tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgcca
      481 atagaagcac cgttgcccgg agcagcagtc aggcgcgcgg tgatcgggtc acctgccagg
      541 gtgctggcgc tcaccatttc cggagagagc ttagacagcg ccgctttttg tgcggaagtc
      601 gcagctgcct gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag
      661 tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacatgatg
      721 atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgca
      781 ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac catcgacaaa ccatttgaaa
      841 cctaccggga cttctaccag tttacggccc aagtcgttga ccacacggtc gatcatcgca
      901 gatgaaacca gcgttttacc gacggcaaca tctttgcccc actgcggacg atgctggaac
      961 aggtaattga ttgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg
     1021 ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcagt
     1081 gccagcaggc ccgccatcgc acactcggag gagcagtcca tacggatcgc gccgtcttta
     1141 tcaaggtgca taaagcggaa ggtttgatcg acctgatcgt taacgatagt caggttgagg
     1201 ttgtaatact cgccaatacg cttccagtat tcgataccgg aaccgcccag cggatcaacg
     1261 cccagcgtca ggcccgcttt ctgaatcgcg gccatatcaa cgatatcggc cagaccttcc
     1321 acgaacggct gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg
     1381 gagatacgct tcacgccttt caggccatcg gccagcagtg cgttggccct gtcttccacc
     1441 actttagtga cgttggtatc agccgggcca ccatttggcg gattgtattt gattccacca
     1501 tcttccggcg ggttatggga cggtgtaatc acgataccgt ctgccagcgg gccacctttt
     1561 ttattgtgaa ccaggatggc attggaaacg gcaggcgtcg gggtgaagcc attgttttcc
     1621 tgcacaatga catcaacgcc gttcgctgcc agcacttcca gaacggaaat gaatgcaggt
     1681 tcggacaggg cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca
     1741 cgttcttcag caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc
     1801 gctgcactgc cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct
     1861 tctggtttca gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt
     1921 tgtgcaggtt ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt
     1981 ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat
     2041 gtgttcgatc atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt
     2101 gcctggcaca tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc
     2161 cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc
     2221 gtcaagagaa tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc
     2281 tgcgtattca tccgaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt
     2341 gaccggcttc gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc
     2401 agcaggctgt gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc
     2461 gccgatatgc ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt
     2521 tttcatctta atccagtgca gtgtcttagc tgaatacaag tcgccaggag tatatgaata
     2581 aatcccggca gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt
     2641 tgttgcagcc ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa
     2701 gaactttgcc atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc
     2761 tcccatcgtt cttgtccacg gtctgtttgg caccctcgac aaccttggcg tactggctcg
     2821 cgatctggta aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc
     2881 gagagatccg gtaatgaatt acccggcgat ggcgcaggat cttgttgata ctctggatgc
     2941 acagcagatc gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc
     3001 acttactgca ctagcctccg atcgcatcga taaactggtg gcgatcgata tcgcgccggt
     3061 cgactatcac gtacgccgtc atgatgagat ttttgcggct atcaacgcgg tcagtgaatc
     3121 ggacgcacaa actcgccagc aagcagcagc aataatgcgc cagcatctta atgaagaagg
     3181 ggtgattcag tttctgctga aatcttttgt tgacggggag tggcgcttta acgtgccggt
     3241 attgtgggat cagtatccgc atattgtagg ttgggagaaa atcccggcat gggatcaccc
     3301 tgccctgttt atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt
     3361 actggctcaa tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca
     3421 tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa
     3481 ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg
     3541 cgctgatgta ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg
     3601 aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc
     3661 acgagcgtcg accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta
     3721 ttaataaacg caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga
     3781 agctgaacgc ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc aatatgagcc
     3841 gtagcgaact gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca
     3901 tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg
     3961 caagactgcc tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc
     4021 aataagtttc aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac
     4081 accggtaata ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc
     4141 gatgtccatg acattgcaaa aagcagcaaa gaagatctgg aagcttatga cattctgctg
     4201 ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg actgggatga cttcttcccg
     4261 actctcgaag agattgattt caacggcaaa ctggttgcgc tgtttggttg tggtgaccag
     4321 gaagattacg ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg
     4381 cgcggtgcaa ccatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa
     4441 ggtctggcag atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa
     4501 ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac
     4561 gaaattctca atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat
     4621 aatgccaatc aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt
     4681 cccggtgttc aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa
     4741 tatttgccag ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta
     4801 tctcaagagc aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga
     4861 ttccgcatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct
     4921 cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat
     4981 ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta
     5041 ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc
     5101 gtatttgaac tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag
     5161 gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat
     5221 ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc
     5281 cgcgaagatg aacatgcgca cgaaggcaaa taagccagcc tgaacgagaa aagccaacct
     5341 gcgggttggc ttttttatgc aagggaaata agaaaagcga gagttacagc tctcacttat
     5401 ttgttattac tgcgtatttc ctgccagact ttatcacaat cggctttcac cgcctgatca
     5461 tttccggttt gcgttgcacg cagacactgc tgataatcca gtacgcgtac actttcctgg
     5521 tcggcaaact gctgatgttg tttctctttc ttcagcacgt ttaacacgct ctggcaggct
     5581 tcaatttttt ctggcgaacc ttgtgcggtg ttgatacagg cgctgtatgc ctctttcaga
     5641 cgactgtctt cctgtggcgc ggttgattgc gcacaagcta ccagccccga tgccattatg
     5701 gcgatgagaa tcagtttttt catagcaaat tcctcaaacg tgcggcaggc acaaaacctg
     5761 ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc ataaatttca cgtcacgctc
     5821 atcctggaag atgttgccgt aaccaccgcc ccagcttggg atgtcggagt ggttgtcgta
     5881 ttcggtgaaa tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa tggtatagac
     5941 cgcatccagg ctgtaggcag actcttcaat agtccggttt ttgtcgtagt acgcatccgg
     6001 attgctctgc caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc
     6061 gaagcctgga agattccagt ttttcaggtc atacatcgca ccgaagaaga ccgctttttc
     6121 gccgttggcg ttgaagtcag aacggttatc ccaccagata tccaggcgac cgtttgagga
     6181 agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc tgaccgtcag ccttaaccca
     6241 ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg taaccaaagg tcaacgcttg
     6301 cagccaggcg gtgccgtcat aaaggtcgtt gacgctgcga tcgtcaactt tatctcgggt
     6361 accgtagaac tggtagctgg tggttaacgg gctaccggcg atatcaaatt tgtagctggc
     6421 tttggcaaaa tattgatcga tatacccttc cgcctgacca aatgccgctt ccagtacgaa
     6481 gttatttttg aagtcgtatt tcgccccaaa ggagtgcaga taatcaactt tggtggtttt
     6541 atcgttctga taaaactcat ccatttccag atgccacggc gctttgtatt cgttggtcca
     6601 catgtaggag aaactcaacg caccagcatc gccgtaatca aaattcgccc cggcttccgc
     6661 cccctgataa gtacctggca taaagctcca gtgcggcgct aacagcgttt gaccagttgg
     6721 ctgaatgtaa cctgccctcg cccaaaccgg accgtattta aatttggccg cagctttata
     6781 caggcttata ccgcttttgt cgccggacca gtcttcgtca taggctttat tactttttga
     6841 aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc gccatttcaa tcgccgtaaa
     6901 cgcagcaata tcaaggccga acatatcagc agcataaccg gactgaaaat cgaggttggc
     6961 attccaggtg gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa catctttacg
     7021 gtcgcgttca cgctgccagt aatagatacc gccggttaag gttgaatcgt cgatgaatcc
     7081 ttcggcgcga gcctccggca ttgccataaa gcccgacatt gctgtaacac cggcgatagc
     7141 cagcgccagc gtactacgtt tgccactaaa cgtacgcatg ggttaatcct ctttgacgta
     7201 taaattgctg caccaaaggt gaacagcgaa aaaataaaaa ttaaaaaagt agcgttgaga
     7261 aaagtctcgc tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc
     7321 tgtaaccaga atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg
     7381 cttaagtttg cggttgaaag gcttgcagaa gaataaaagc gtgaaattcc agcaagatga
     7441 aag
//
LOCUS       DBBGLQ010000162         7389 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.162, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000162 DBBGLQ010000000
VERSION     DBBGLQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.162"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..402
                     /gene="istB"
                     /locus_tag="ACIYSB_004889"
     CDS             <1..402
                     /gene="istB"
                     /locus_tag="ACIYSB_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HGC5431752.1"
                     /translation="VRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLII
                     DETGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRI
                     LHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            complement(618..1349)
                     /locus_tag="ACIYSB_004890"
     CDS             complement(618..1349)
                     /locus_tag="ACIYSB_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGC5431753.1"
                     /translation="MNQPIHNDYWLSRFESILNSALVQHRAVSLIRVDLRFPEHMPVT
                     IMDPDPDSAVISRFFESLKAKIQAYQRKKRRTNKRVRATTLHYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPA
                     SSDARPSSDDTPLSGGCSETRKASDKKPGEAAVLWIKRGDVEAMQKAMERARYLVKYE
                     TKQHDGSGQRNYGCSRGAGRLLDGR"
     gene            2019..2549
                     /locus_tag="ACIYSB_004891"
     CDS             2019..2549
                     /locus_tag="ACIYSB_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGC5431754.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAVMAITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            4169..5023
                     /locus_tag="ACIYSB_004892"
     CDS             4169..5023
                     /locus_tag="ACIYSB_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory RepB family protein"
                     /protein_id="HGC5431755.1"
                     /translation="MSVHSPDESDHVFSRMEYEYYRHNDKSPSVNADNAGRDVVPEWV
                     QKDPVAAFWVWMFIYHVPDSIVRLSGEQYADYGTSCLYARLFATEAAPANHQARIRVT
                     RKWLQELIRLTGTENVSDFLYVVHFLWSYAEKVNPMTWLGSRRIHIKWAWAYLQKHCT
                     DEERHCPDISEFKASGESRLFIIAYVRRVFMKIYSVGTEPQKLKELVYQQNDFLRRMK
                     CSYQKMFRYEKTDSRVQISALISPEARNALRKMAKKNNLTQAEMLDKLILSADRLFMQ
                     KTAGDYSR"
     gene            complement(5020..5982)
                     /locus_tag="ACIYSB_004893"
     CDS             complement(5020..5982)
                     /locus_tag="ACIYSB_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431756.1"
                     /translation="MTAQQSDALREIANKARVTTILQCKAWKDTQRILKRSGLVCRER
                     SEPFDPEKHFDCYTVRYLYLLNIMALELKSDTRIKVEVGQWYRMTGKRLSLNVPPFML
                     IPRNIRRKVDGFRQSRQSEDEATKNPPQPFTGSLYKVLSRDSDSAELDAWFAEPPLTR
                     QEVWEGRRVTDFDPWALSSFICRSESPTFELFYQEYKRLGLKSLFVSGVMFEQFLTGL
                     SFRKYGDWVESQLLESLGNVMFFMLLYDMENLDKFIKELMDINVQSEDSKEKGKSRKE
                     RMLEYINSYIRNVYGRFLCTSKERYEQHKRKNSSKKKNGSGGTH"
     gene            6088..6972
                     /locus_tag="ACIYSB_004894"
     CDS             6088..6972
                     /locus_tag="ACIYSB_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGC5431757.1"
                     /translation="MSFSHSSLSAQVKSHLTFLPEEIRQKILEHLHSVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQFGERRMTLVDLPGIGETPQH
                     DQEYRTLYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQSLSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIHLLITLWERLF"
     gene            7175..>7389
                     /locus_tag="ACIYSB_004895"
     CDS             7175..>7389
                     /locus_tag="ACIYSB_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HGC5431758.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSP"
BASE COUNT         2127 a         1724 c         1567 g         1971 t
ORIGIN      
        1 gtccgtgcag gtatcaaagt tcgcttcaca acagcagcag atctgttact tcagttatcc
       61 acggcgcaac gtcaggggcg ttataaaacg acgcttcagc gtggagtaat ggccccccgc
      121 ctgctcatca ttgatgaaac aggctatctg ccgttcagtc aggaagaagc aaaactgttc
      181 ttccaggtca tcgctaaacg ttacgaaaag agcgcaatga tcctgacatc caatctgccg
      241 ttcgggcagt gggatcaaac gttcgccggt gatgcagccc tgacctcagc gatgctggac
      301 cgtatcttac accactcaca tgtcgttcaa atcaaaggag aaagctatcg actcagacag
      361 aaacgaaagg ccggggttat agcagaagct aatcctgagt aaaacggtgg atcaatattg
      421 ggccgttggt ggagatataa gtggatcact tttcatccgt cgttgacagt ctggcatcgc
      481 agcgacaaat gatggatgca tgaaaaccag gaaacattgc atgacttcag gacgactttt
      541 tcagaaaacg agctaagggc gtcaaccacg cccttactgt gacatatact ctggcaccgg
      601 atgccgtttt acagggttca cctgccatcc agtagacgcc ccgctccacg gctgcaacca
      661 taattacgtt gaccagaacc gtcatgctgc ttcgtctcat acttcacgag ataacgggct
      721 ctctccatgg ctttctgcat cgcttccaca tcaccacgct tgatccagag aacagcggct
      781 tcacccggct ttttgtctga agccttcctg gtttcagaac atccacccga caaaggcgta
      841 tcatcggaag aagggcgagc atcagatgat gccggtttac ggaaaggcgt ccgcctggaa
      901 aaatgaacca gtccattacc ctgccagggc tcaagatgca gagcgctaca ccatgccagt
      961 tggatcagcg tcgccagcga agaaggaacg gtgaaatccc ctggcgagca ccaggtatct
     1021 ttgttgagca gtaatatcac gtgataatgt ttcctgcctt tttccttgcc aaactcccga
     1081 caccagaaat aatgcagggt ggttgcacgc acacgcttgt tggtacgtcg ttttttccgc
     1141 tggtaagcct gaattttggc tttcagggat tcgaaaaaac gagaaatcac cgctgaatcc
     1201 ggatcgggat ccatgatggt gacaggcata tgctcaggga aacgtaaatc cacccggatt
     1261 aacgagacgg cacggtgttg caccagggca ctgttgagaa tactttcaaa acgggataac
     1321 cagtaatcat tgtgtattgg ttgattcatg atgagagatc tccgttgatt tggattgact
     1381 tcacatcatt aatcgccgcg gtaaaacttt acggcatccc accaatgagc aacgccaata
     1441 gctcctgatt aaaacggaat gaatcgtccc gtaacgaaaa gataatcctg cacacgacag
     1501 caaaacgtaa aacaacagca tgaatgagct aaatgtctaa agaaaaactg ccttttcaca
     1561 ttatttttca gcgagtaatc ataccagcat ccctaacata cgaaatcgca tcgtgatcga
     1621 tcgctgattc gccgaacaac tgatcgaata caccggaatc tgcactaaca tcctgatggg
     1681 taacggaaga ctaaaaaacc agtgttctat tgattgcacg gaatgcctgt gcgctggcct
     1741 gaccagtaag cacaattcta cgagtagtaa gatatctact ccaaaaccat gcgcagtagc
     1801 caccatatgt tgccagggtg aaaaataagc tcagagctat aggggagtaa ttcccgttaa
     1861 tgtcatcgcc gtacctcaat cagctcacaa actggctgaa acgttaagct gaggccttta
     1921 ttcttcagat gtattttccg gtttacctgt aaattatctt ctgatattta tcacattagt
     1981 aattgtgctg attatctaca tacttcaagg tctgaaatat gaagctaaca gaagaaagtc
     2041 tgaatctagt cattgacatc ctgaatgatt tgatctgtga aggaaaaatg ctgaacgatg
     2101 tggagcgcag atctctgctc aatgcagtta tggcaattac agcggtaaaa gaacgttctg
     2161 tcgtatctgt atcatcccga aaagagcaaa gtaagaagga gaagaaggaa agagagatcg
     2221 atcctcgttt cccaaatgct ggtgtggcat ggaatgaggc agacgagagt ttattgcgag
     2281 atgttctgga ggatgtaccg gacgatgaaa ttggtaaaca ccttttctgg ctggcagaga
     2341 aactagggcg aaccccgtac agtgtagcct gtaaagtcag acaaattaag aaattacctg
     2401 cagaatggaa ggatcaattt cgtaaaatct ctgacaggat ccgtagttct ggaatgtcag
     2461 tcagtgaata tctacaacgc cagggactgt taagcccaga gtcaggggaa gaaaacaatc
     2521 ctgtcagtca taacaacaat gtagcttaat cctgaagaag atgaacagtt tatactcttt
     2581 aaactgttcc cttaatttta gaaaaacagt ttgtgtaagt gcatgctcca acattctgaa
     2641 ggcgttattt cttacaatac atcataatca ttggcagatc ctcatctgaa tcaaaacacc
     2701 acaaccaatc atcacacttt ttctgaaaag agccttatga tgaagataat caccttacgc
     2761 tgagaaaaaa caccagggcc agttaatcac cttatcaatc tcgccgttac aaaatcatcc
     2821 ataagccatt taacagtgtt atcgcaaact cacagtaaat cgcatcaggt tacctcaatc
     2881 ggatttgagt taattcactg ggctttacgt aaagatactt ttctcaagct aacgtcgaaa
     2941 ctcgaaatct aaggaaaggt taaacgtaac atcagtaaaa tcgaatagca gattgattgc
     3001 ttaaacagaa aggttttaca gcagcattaa actgatttag ttaacgtaaa gatttactaa
     3061 actggatgtg tatagcaatg cttaacgaaa acacttgtag aagtgtttat gaaaatacgt
     3121 gtatgtctgg taaaacactt agtagaaaat acgtgtataa cacttactga aatgcttagt
     3181 aaaaatactt agtaaaatac gtgtatagta ccattcccga cattctcaac atcttcagac
     3241 atccatgcag actgagtgtt gatttcagtt tttcacggcc agcatggatc tttactgttt
     3301 aaaaaacata cagatttact ctgaaattat ttacccaggc ctccctgaaa agaccatccg
     3361 gagcatcccg ttcgtcagct tccatgttgt cttttacccc ggaaagtgat cgcattttcc
     3421 agccagaatg acagccatac tgattatgac gtctctccgg ccccttcaca tataacattc
     3481 atgtcagtca tttcttactg cctcactcac cggaattctc catgccggat gaagcaattg
     3541 ttctgaactg agcagaagca taacagcaca caaggaaacg actgtgctat agatcctgga
     3601 atcaggatct taatgtcagg aaaacaatga cgaccgggga taaccaacaa caacctgagt
     3661 acagaagaaa gctttcccgc gcacgccatc atcttatgta agaggatatg atgccacccc
     3721 cacctcagaa gaaccgtctc cgggagaact tcactgcatc caaaatcctt ttgctgagtt
     3781 ctcattcccc atcatggcag ctacagacat gccctgacag agcgtatgcg cattgagaga
     3841 aataaccata aatgatgaga agacactcta tataacgaca gcatgttgag gcatcaaaaa
     3901 tattcatata aatattttaa gaaaattttt tattgcaaac tacaggaaat aacatgatgt
     3961 caatataatt ttttaaaaaa cgcgatatta atcacattgt aattttctgt gtttttatgt
     4021 aaatcagaga aaagagtcca tgaacgttat tgcatttttt ctgatgtatg gataaaaggg
     4081 gattcaggcg aaatttactg acagaggtga taagaggggg ggtaaagagt tctatagagt
     4141 tggttgtgca tttatgccca gggagttcat gtctgtgcat tcaccagatg aatctgatca
     4201 tgtttttagt cggatggaat atgaatatta ccgacataac gataaatcac cttctgttaa
     4261 tgctgataat gcaggcagag acgttgttcc ggagtgggtg caaaaagacc ctgtagccgc
     4321 attctgggta tggatgttta tatatcatgt tcctgacagt attgtccgac tctcaggtga
     4381 gcaatatgca gattacggaa cttcctgtct gtatgccaga ctgtttgcca cagaagcagc
     4441 tccagctaat catcaggcaa ggattcgtgt gacgagaaaa tggctacagg agcttataag
     4501 gctaaccggg acagaaaatg tatctgactt cttgtatgtc gttcattttt tatggtcata
     4561 tgctgaaaaa gtgaacccca tgacatggct tggttcccgg cggatacata tcaaatgggc
     4621 atgggcgtat ctgcaaaaac attgcactga cgaagaacgt cactgtcctg atatttcaga
     4681 atttaaagct tcaggggagt cacgtctgtt cataatagct tatgtgcgcc gtgtcttcat
     4741 gaaaatctat tctgtcggta ctgaacctca aaagttaaaa gaattagttt atcagcagaa
     4801 tgattttctc aggcgcatga agtgctcata ccagaaaatg ttccggtatg agaaaaccga
     4861 ttcccgggta cagataagtg ccctgatttc tccggaagcc aggaatgcac tccggaaaat
     4921 ggcaaaaaag aataatctga cgcaggctga aatgctggat aaactcatct tatcagctga
     4981 tcgtctgttt atgcagaaaa cagcagggga ttattcccgt taatgagttc caccagaacc
     5041 gtttttcttt ttgctgctgt ttttgcgttt atgctgttcg tacctttctt ttgacgtaca
     5101 aaggaatctt ccataaacat tgcggatata tgagttgata tactccagca ttctctcttt
     5161 tcgtgatttc cctttttcct tcgaatcttc actttgaaca ttaatgtcca tcagttcttt
     5221 tataaactta tccagatttt ccatgtcata aagcagcatg aaaaacatca catttcccag
     5281 tgattccagt aactgagatt caacccagtc cccatatttt ctgaaactta gccctgttaa
     5341 aaactgttca aacattaccc ctgatacaaa aagagatttt aatcccagtc gcttatattc
     5401 ctggtaaaaa agttcaaacg tcggggattc tgaacggcaa ataaacgaag acagcgccca
     5461 tggatcaaaa tctgtaacac gcctcccctc ccacacttcc tgccgtgtca gcggcggttc
     5521 agcaaaccag gcgtccagct cagccgaatc gctgtccctg ctcaaaacct tatacaaact
     5581 gcccgtgaac ggttgaggcg gatttttggt tgcttcatct tctgattgtc tggattgtct
     5641 gaatccgtcc acttttctcc ggatattacg cgggatcagc ataaaaggcg ggacattaag
     5701 tgataaacgt ttacccgtca tgcgatacca ttgccccacc tcaaccttaa tccgtgtatc
     5761 tgactttagc tcaagtgcca ttatgttaag caggtacaga taacgcaccg tataacagtc
     5821 aaaatgtttc tccggatcaa acggctctga ccgttctctg cacacgagtc cgctgcgctt
     5881 cagtattcgc tgggtatctt tccaggcctt acactgaaga atggtggtaa ctctggcctt
     5941 attagcaatc tcacgcagcg catcactttg ttgtgccgtc atctcacctc ccgcactctg
     6001 tcactccaga ttttttcacg tccatattac ctgaatgata agccggatta gtgtgatccg
     6061 gttcagcgaa atcaacagga taacggtatg tcattcagcc attcttccct ctccgcacaa
     6121 gtgaaatcac atctgacgtt tcttcctgaa gaaattcgcc agaaaatcct tgaacatctc
     6181 cacagtgtta ttcattacga gcccgtgatt ggcattatgg gtaaatccgg aacaggcaaa
     6241 agcagtctgt gtaatgccat ttttcagtcc cgtatctgcg ccacacatcc tctgaacggc
     6301 tgcacccgcc aggctcatcg tcttaccctg cagttcggtg aacgcagaat gacgctggtc
     6361 gatctgcccg gcattggtga aacaccgcag catgatcagg aataccggac actttatcgt
     6421 cagctgctac cggaactgga tctgattatc tggatcctgc gggctgatga acgtgcgtat
     6481 gctgctgata ttgccatgca tcagttttta ctgaatgaag gcgcagatcc ctcgcgtttt
     6541 ctgtttgttc tcagccatgc cgatcgcgtg tttcctgctg aagaatggaa tgccacagaa
     6601 aaatgcccgt cccgtcagca gtcgctctca ctggcgacgg tgacagcccg ggtggcaacc
     6661 ctgttccctt catcatttcc ggtactctcc gttgctgcac ctgtcggctg gaaccttccg
     6721 gcgtttgtgt cgctgatgat ccacgcactg ccaccacagg caaccagcgc ggtgtattca
     6781 catatccggg gagaaaaccg ctctgaacag gcccagaaac acgcacaaca gacttttggt
     6841 gaaaccatcg ggaaaagttt tgacgtcgcc gttgcccgtt tcagttttcc ggcctggatg
     6901 ttacagcttc tgcgtaaagc ccgggaccgc attatccacc tgctgatcac actgtgggag
     6961 cgtctgttct gacacactca cgccgacagg atgtgtcgct gcattaacga gcattcttct
     7021 ttttatgaaa tcatgcttaa aaatcagata attaaaagaa tattttttct gctgcatttt
     7081 attcctgatt atccggatgc gacacatcct ttcaacatca tgatgcataa taacatcatg
     7141 aaataaaaga tgttttctta cggagtgcac atctatgtct gataatcgtt cccggcatga
     7201 tcgcctggcg gttcgcttat cactcattat cagccgactg atggccggag aatctctgtc
     7261 actaaaaaca ctgtcagatg aatttggcgt tacagaacgt actttacagc gcgattttca
     7321 tcagcgtctg gttcacctgg atttagagta cagaaatggc aggtacagcc tcagacgaca
     7381 gagcagccc
//
LOCUS       DBBGLQ010000163         7296 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.163, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000163 DBBGLQ010000000
VERSION     DBBGLQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.163"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..242)
                     /locus_tag="ACIYSB_004896"
     CDS             complement(<1..242)
                     /locus_tag="ACIYSB_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGC5431759.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACIYSB_004897"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACIYSB_004897"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGC5431760.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKTQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2254..3903
                     /locus_tag="ACIYSB_004898"
     CDS             2254..3903
                     /locus_tag="ACIYSB_004898"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGC5431761.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3954..4556)
                     /locus_tag="ACIYSB_004899"
     CDS             complement(3954..4556)
                     /locus_tag="ACIYSB_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGC5431762.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5076..5975
                     /locus_tag="ACIYSB_004900"
     CDS             5076..5975
                     /locus_tag="ACIYSB_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5431763.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLTAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            6024..7037
                     /gene="yhjD"
                     /locus_tag="ACIYSB_004901"
     CDS             6024..7037
                     /gene="yhjD"
                     /locus_tag="ACIYSB_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGC5431764.1"
                     /translation="MTQENEIKRPIQDLEHEPIKPLDNSEKGSKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
BASE COUNT         1877 a         1859 c         1705 g         1855 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctgaaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt gctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccgcgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacttcga ggaactgcgt tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa acccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attacgatgt tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat
     1501 tcaatcttat tcaaatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcaaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagggagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac caaatcggta
     2101 tttttataaa ttccattgat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagccga
     2221 taattagcga cgaatttcgg aggttggatc cttatgctca atcagaaaat tcaaaaccct
     2281 aatccagacg aactgatgat cgaagtcgat ctctgctatg agctggaccc gtatgaatta
     2341 aaactggatg agatgatcga ggcagaaccg gaacccgaga tgattgaagg gctgcctgcc
     2401 tctgatgcgc tgacgcctgc cgatcgctat ctcgaactgt tcgagcatgt tcagtcggcg
     2461 aaaattttcc ccgacagtaa aacctttccc gactgcgcac ctaaaatgga cccgctggat
     2521 atcttaatcc gctaccgtaa agtgcgccgt catcgtgatt ttgacttgcg caagtttgtt
     2581 gaaaaccact tctggctgcc ggaggtctac tccagcgagt atgtatcgga cccgcaaaat
     2641 tccctgaaag agcatatcga ccagctgtgg ccggtgctaa cccgcgaacc acaggatcac
     2701 attccgtggt cttctctgct ggcgctgccg cagtcatata ttgtcccggg cggccgtttt
     2761 agcgaaacct actattggga ttcctatttc accatgctgg ggctggcgga aagtggtcgg
     2821 gaagatttgc tgaaatgcat ggccgataac ttcgcctgga tgatcgaaaa ctacggtcac
     2881 atccccaacg gcaaccgcac ctattatttg agccgctcgc aaccaccggt ttttgcgctg
     2941 atggtggagt tgtttgaaga agatggtgta cgcggtgcgc gccgctatct cgaccacctt
     3001 aaaatggaat atgccttctg gatggacggt gcagaatcgt taatccctaa tcaggcctat
     3061 cgccatgttg tgcggatgcc ggacggatcg ctgctcaacc gttactggga cgatcgcgac
     3121 acgccgcgtg acgaatcctg gcttgaggac gttgaaaccg cgaaacattc tggtcgcccg
     3181 cccaacgagg tgtaccgcga tttacgcgcg ggggcggcct ccggttggga ttactcttcc
     3241 cgttggctgc gtgatactgg tcgtctggcg agcattcgta ccacccagtt catccccatc
     3301 gatctgaatg ccttcctgtt taaactggag agcgccatcg ccaacatctc ggcgctgaaa
     3361 ggcgagaaag agacagaagc actgttccgc cagaaagcca gtgcccgtcg cgatgcggta
     3421 aaccgttacc tctgggatga tgaaaacggc atctaccgcg attacgactg gcgacgcgaa
     3481 caactggcgc tgttttccgc tgccgccatt gtgccactct atgtcggtat ggcgaaccat
     3541 gaacaggccg atcgtctggc aaacgccgtg cgcagtcggt tactgacacc tggcgggatt
     3601 ctggcaagcg agtacgaaac cggtgaacag tgggataaac ccaacggctg ggcaccgtta
     3661 caatggatgg cgattcaggg atttaaaatg tacggcgatg accttctggg tgatgaaatc
     3721 gcgcgaagct ggctgaagac ggtgaatcag ttctatctgg aacagcacaa actgatcgaa
     3781 aaataccata ttgccgatgg tgttccccgc gaaggcggcg gtggcgagta tccgttgcag
     3841 gatgggtttg gctggactaa cggtgtggta cgccgtttaa ttggtttgta cggcgaacca
     3901 taatattttt acagccagcc gctaacttcc tgctggctgt aaaattatcc tcttcaggag
     3961 gagatattta acatcattgc cgcctgggtg cgatttttca cttccagacg gcgatacagg
     4021 gattccagat gcgcttttac cgttccggta ctgatattca gcgctctgcc gatctcctta
     4081 tttgattcgc ccgccgctaa catggttaaa atctcccgct ggcgggcgct taacgatttg
     4141 agatctttaa tgtccttttc cggcgtcgtc cgccagtctc caggcagaaa catcatcccc
     4201 atcgccgcac tatttaccgc caacgcaaat gtctcgacgg ttgaatcacg aggcacaatg
     4261 gccagcacat taaaatggat aacttcctgt aaccaccgtt tattgcaatc cgtcgccgta
     4321 attaacacct taacctcagg aaattgcacc acggtttttt gcagcaacca gtagcaaaac
     4381 tcaccatcct gatcgccatc gagcataact aaggcttcag ggtaactttc cagcttttgc
     4441 cataactcgt ctgcctgact ggccccctga atactcactc ctggaatacg ctgctgtaaa
     4501 ctgattttca ttccatgaat aaatattgac tgcctgtcaa acatgactat ttgcataact
     4561 gaatctccac ctgaatacgt taaaaagact taagtagtgg aagggtatta cccgcgagaa
     4621 aaaataagaa ttcgccattt ggcggtggcc attctacaga gatgacgtgt agaaaatagt
     4681 taccgatata aatagttaca gctaaacgcc tgaaattaca tgtcgagggc actatttaaa
     4741 acaattttga ggatttcctt atattggtgg ttagtacgca tgcaattaaa aatgaaattc
     4801 cgcgaccaca agccaaaata acaaacggca aggagacaaa aataagcaca aatagccaac
     4861 acgtcctctg ttcactttaa agggaatcgc tgaaaaatac gctctgttta aggggattca
     4921 cctttctcag aaagctattc cgcccttttc ctgctgagaa atcgccacat tcggcatgac
     4981 aacattgtga aacccggcat tagatgttag aaaaaacaat aaacaatgcg atatgcgcgt
     5041 tactgtttcg ctgtgaacaa caatcggtca aagaaatgga taaaattcac gcaatgcagt
     5101 tgttcatcaa agtcgcggag ctggaaagtt tttcccgcgc agcggatttc tttgctttgc
     5161 caaagggaag tgtttcgcgc cagatacagg cactggaaca tcaacttggc acccagcttc
     5221 tccagcgcac cacgcgacgg gtcaaactca cgccagaagg catgacctat tatcaacgag
     5281 caaaagatgt gttgagtaat ctcagcgaac tggacggtct gtttcaacag gatgccacca
     5341 gtatcagcgg taaattacgc atcgacatcc cgccaggaat cgcgaaaagc ctgttactgc
     5401 cgcgcctgtc ggaatttctc tatctgcatc cgggaattga gctggaactg agtagccatg
     5461 accgtccggt agatattctt catgatggtt ttgattgcgt gatacgcact ggcgcgttac
     5521 cggaagatgg cgttatcgcc cgtcccctcg gcaaactgac catggtcaac tgtgccagtc
     5581 cgcactatct gacgcgcttt ggttatccgc aaagccccga tgatctgact tcacacgcaa
     5641 tagtgcgtta tacaccgcac ctgggtgtac atccgttagg ttttgaggtt gccagcgtta
     5701 atggcgtcca gtggtttaag tctggcggca tgttgacggt aaacagtagc gaaaactatc
     5761 tcaccgccgg tcttgccggt ctggggatta ttcagatccc gcgcattgcc gtgcgcgaag
     5821 ccctgcgtgc cgggcggctt attgaagtat tacctggcta ccgtgccgag ccgctctccc
     5881 tttcgctggt ttatccgcag cgtcgggagc tttcccggcg tgtaaacctg tttatgcagt
     5941 ggctggctgg cgtaatgaaa gagtacctgg actgaccgac tatacttttt aagaatgacc
     6001 acgacagaag gacaaaagag cggatgacgc aggaaaacga gatcaaacgt cccatccagg
     6061 atctggagca cgagccgatt aagccgttgg ataatagcga gaaaggcagc aaagttagcc
     6121 aggcgctgga aaccgtcacc accactgccg aaaaagtcca acgccaaccg gttattgcgc
     6181 acctgattcg cgcgacagaa cgctttaacg atcggctggg taaccagttt ggtgcggcta
     6241 tcacctattt ctcatttttg tcgatgatac caattttgat ggtgtcgttt gccgcagggg
     6301 gctttgtgct ggcctcccat ccgatgttgc tacaggatat cttcgacaaa attctgcaaa
     6361 acatcagcga tccgacgcta gccgccacgt tgaaaaacac catcaacacc gccgttcagc
     6421 agcgtacgac tgtagggctt gtcggcctgg cggtggcact ttattccggc atcaactgga
     6481 tgggtaacct gcgtgaagcg attcgtgccc agtcgcgcga tgtctgggaa cgctcgccgc
     6541 aagatcagga gaagttctgg gtaaaatatc tgcgagattt tatttcgctg attggtttgt
     6601 tgattgcgct gattgtgacg ctttcgatca cctcggttgc cggttcggcg cagcaaatga
     6661 ttattagcgc cctgcacctg aacagcattg agtggctgaa accgacgtgg cgattgattg
     6721 gcctggcgat ttccatcttc gccaactatc tgcttttctt ctggatcttc tggcggttgc
     6781 cgcgccaccg tccgcgcaaa aaagccctga ttcgcggaac atttctggct gctattggtt
     6841 ttgaagtgat taaaatcgtg atgacctaca ccctgccatc gttgatgaaa tccccctctg
     6901 gcgcagcatt tggttccgtg ctggggctga tggcattttt ctacttcttc gcccgtttga
     6961 cgctgttttg cgcggcgtgg attgccaccg ccgaatataa agacgacccg cgaatgccgg
     7021 ggaaaacgca gccttaaaat aggccggatg cggcgtaaac gccttatccg gcatacgatt
     7081 ttattcggcc tggctccccg taggccggat aagatgcgcc agcatcgcat ccggctataa
     7141 tgcgcacata acctcttgaa actcgtcccc agagcctctt cagccatcta tttgggagca
     7201 aacaatttca ttccaactca taaccccagc atataaatcc agttggtaac ttttatttaa
     7261 cctgaaacca gttttatcca ctatttataa aattat
//
LOCUS       DBBGLQ010000164         7224 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.164, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000164 DBBGLQ010000000
VERSION     DBBGLQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.164"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..645
                     /locus_tag="ACIYSB_004902"
     CDS             <1..645
                     /locus_tag="ACIYSB_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGC5431765.1"
                     /translation="IFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEA
                     ALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQ
                     INEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGA
                     FFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            696..1046
                     /gene="chbA"
                     /locus_tag="ACIYSB_004903"
     CDS             696..1046
                     /gene="chbA"
                     /locus_tag="ACIYSB_004903"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGC5431766.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1054..1896
                     /gene="chbR"
                     /locus_tag="ACIYSB_004904"
     CDS             1054..1896
                     /gene="chbR"
                     /locus_tag="ACIYSB_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGC5431767.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVALSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2001..3353
                     /gene="celF"
                     /locus_tag="ACIYSB_004905"
                     /gene_synonym="chbF"
     CDS             2001..3353
                     /gene="celF"
                     /locus_tag="ACIYSB_004905"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGC5431768.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGQIDNIPADWAVEMTCKLGRDGATLHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            3366..4115
                     /gene="chbG"
                     /locus_tag="ACIYSB_004906"
     CDS             3366..4115
                     /gene="chbG"
                     /locus_tag="ACIYSB_004906"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGC5431769.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELVSQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEQGIALRADRQMAFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDAGHRGDRSLEVMCHPAFIDNTIRQSAYCF
                     PRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(4373..6634)
                     /gene="katE"
                     /locus_tag="ACIYSB_004907"
     CDS             complement(4373..6634)
                     /gene="katE"
                     /locus_tag="ACIYSB_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGC5431770.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            6817..7080
                     /gene="cedA"
                     /locus_tag="ACIYSB_004908"
     CDS             6817..7080
                     /gene="cedA"
                     /locus_tag="ACIYSB_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGC5431771.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1790 a         1633 c         1906 g         1895 t
ORIGIN      
        1 atctttgttc cactgctctg gttcttcggt attcatggcg cgctggcgct gaccgcactg
       61 gacaacggca ttatgacgcc gtgggcactg gaaaatatcg cgacctatca gcaatatggt
      121 tccgtcgaag cggcgctggc agccggtaag accttccata tctgggccaa gccgatgctg
      181 gactccttta ttttccttgg gggcagtggt gcgactttag gcctgatcct ggctatcttt
      241 atcgcctctc gccgtgctga ttatcgtcag gtggcaaaac tggcgctgcc gtccggcatc
      301 ttccagatta acgaaccgat tctgtttggt ctgccaatta tcatgaaccc ggtgatgttt
      361 atcccgtttg tactggtaca accgattctg gcggcaatca ccctcgcagc gtactacatg
      421 ggcattattc ctccggtgac caatattgca ccgtggacca tgccaaccgg tctgggagcc
      481 ttctttaaca ccaacggtag cgtcgccgca ttgctggtcg cactcttcaa ccttggcatc
      541 gcaacgttaa tttatctgcc ctttgttgtg gtggctaaca aagcacaaaa tgcgattgat
      601 aaagaagaga gcgaagaaga tatcgctaac gccctgaaat tttaatcgat gccggtacgg
      661 gtaatcgtgc cggtaagaaa agagaggaac gatgtatgat ggatctcgat aacattcccg
      721 atacgcaaac ggaagctgaa gagctggaag aagtggtgat ggggctgatc atcaactccg
      781 gacaagcgcg cagcctggcg tatgcggcac tgaaacaggc gaagcagggc gattttgccg
      841 cagcaaaagc catgatggat cagtcacgga tggcattgaa tgaagcgcat ctggtacaga
      901 cgaaactgat tgaaggcgat gcgggcgaag gtaagatgaa agtgagtctg gtgctggtcc
      961 acgctcagga tcatttaatg acgtccatgc ttgcgcgtga actgattact gaattaattg
     1021 agcttcatga aaaactgaag gcataaggag tcgatgatgc agccagtgat taacgcgccg
     1081 gaaattgcca ctgcccgaga acagcagttg tttaatggca aaaacttcca tgtgtttatc
     1141 tataacaaaa ctgagagtat cagcggactg catcagcacg actattatga atttactctg
     1201 gtattaaccg ggcgttattt ccaggagatt aacggtaagc gcgtgttact ggaacggggc
     1261 gattttgttt ttattccgtt aggttcgcac catcaaagtt tttatgagtt tggtgccacg
     1321 cgcatattga acgttgggat cagtaaacgc ttttttgagc agcattacct gccattgttg
     1381 ccttattgct ttgtcgcttc gcaggtatac cggaccaata acgcttttct cacctatgtg
     1441 gaaacagtga tttcttcatt gaatttccgc gaaacagggc tggaagagtt tgttgagatg
     1501 gttacttttt atgtcattaa ccgtttacgt cattaccgcg aagaacaggt gattgatgat
     1561 gtaccgcagt ggctgaaaag tacggtagaa aagatgcatg ataaagagca gtttagtgaa
     1621 tcggcgctgg agaatatggt ggcgttgtca gccaaatcac aggaatattt gacgcgagcg
     1681 actcaacgat attatggcaa aacgccaatg cagattatta atgaaatccg tattaatttt
     1741 gccaaaaaac aactggaaat gaccaactat tcagtgacgg atattgcgtt tgaggccggt
     1801 tatagtagcc cgagtttgtt tattaaaacg tttaagaaat taacgtcctt tacgcctaag
     1861 agctatcgta agaaattgac tgaatttaat cagtaagtcg ttatacctga caattcacat
     1921 atctgtcctg ttgctggatt atttatgtcc gggggggcag atatgccaga tatcagtatt
     1981 ctgtactgaa gggagaaatt atgagccaga aattaaaagt cgtcactatt ggtggcggga
     2041 gcagctatac cccggagtta ctggaaggat ttattaagcg ttatcacgaa ttgccggtca
     2101 gcgaattatg gctggtggat gtcgaaggtg gtaaagcgaa actggatatt attttcgatc
     2161 tctgccaacg gatgattgat aacgctggcg tcccgatgaa gctttataaa acgctggatc
     2221 gccgcgaagc attgaaagat gctgatttcg ttactaccca actgcgcgtt ggccaattac
     2281 cggcgcgtga actggatgaa cgtattccat taagtcatgg ttatcttggt caggaaacca
     2341 acggcgcggg cggtttgttt aaaggtctgc gtaccattcc ggtgattttt gacatcgtaa
     2401 aagatgtcga agaactttgt ccgaatgcat gggtgattaa cttcactaac ccggcgggaa
     2461 tggtcactga agccgtttat cgtcataccg gatttaaacg ctttatcggc gtgtgtaata
     2521 ttccgatcgg catgaagatg tttattcgcg atgttctgat gctgaaagac agcgatgatt
     2581 tatctatcga tttgttcggc ctcaaccata tggtgttcat taaggatgtg ctggtaaatg
     2641 gcaagtcgcg ctttgccgaa ttgcttgatg gtgtggcgtc agggcagtta aaagcgtcct
     2701 ctgtaaaaaa tattttcgat ctgccattta gtgagggctt aattcgttcg ttgaatctgc
     2761 tgccatgttc ttatctgctg tattacttca agcagaaaga gatgctggct attgaaatgg
     2821 gcgaatacta caaaggcggc gcacgagcgc aggtagtaca gaaagtcgag aaacaacttt
     2881 ttgagctgta taaaaatcct gagctgaaag ttaagccgaa agaactggaa cagcgcggtg
     2941 gggcttatta ctctgatgca gcatgcgaag tgatcaacgc tatctacaac gacaagcaag
     3001 cagaacatta cgttaatatc ccgcatcatg ggcagattga taatattccg gcagactggg
     3061 cagtagaaat gacctgtaag ctggggcgcg atggcgcgac gctacatccg cgcattacgc
     3121 atttcgatga taaagtaatg gggctgattc acaccattaa aggcttcgag attgctgcca
     3181 gtaacgccgc acttagcgga gaatttaacg atgtgttact ggcgctaaac cttagtccgt
     3241 tggtgcattc cgatcgcgat gctgagctgc tggcacgcga gatgattctg gcgcacgaga
     3301 aatggctgcc aaactttgcc gactgcatcg cagagcttaa aaaagcacat taaccgaggc
     3361 tgattatgga acgcttactg attgttaatg ccgatgattt tggcttaagc aaaggtcaga
     3421 actacggcat tatcgaggcc tgtcgcaatg ggattgtcac gtcgacgacg gcgctggtga
     3481 atgggcaggc tattgaccat gcggtgcagt tgagtcgtga tgaaccgagt ctggccatag
     3541 ggatgcactt tgtccttact atgggtaagc cactgacagc tatgccgggg ttaacccgcg
     3601 atggtgtgct gggaaaatgg atctggcagt tggcagaaga agatgcttta ccgctggaag
     3661 aaattactca ggagcttgtc agtcagtatt tgcgtttcat tgagctattt ggacgcaaac
     3721 ctacgcatct tgatagccat catcatgtgc atatgttccc gcagattttc ccgattgttg
     3781 ccaggtttgc ggcagagcag ggcattgcgt tgcgggcgga tcgtcagatg gcgtttgatt
     3841 tgccggtcaa cctgcgcact acccagggat tcagcagtgc attctacggt gaagagatca
     3901 gtgagtcgct gttcctgcaa gtgctggatg acgcaggcca tcggggtgat cgttcgctgg
     3961 aggtgatgtg tcatccggcg tttatcgata atactatccg tcagagtgct tactgtttcc
     4021 cacgcttaac ggagctggat gtgttgacat cagcgtcgtt gaaaggtgct atcgctcagc
     4081 gtggttatcg gttggggagt tatcgggatg tgtaagtgag gatgtttgcc agatgcggcg
     4141 tgaaccttgc atccggcgat taattcctac aaaggcggcg taactgcgcc gcttgagact
     4201 gctgacaaac gcaaaactgc ctgatgcgct tcgcttatca ggcctacgtg tttcctgcaa
     4261 tatattgaat ttgcacagtt ttgtaggccg gataaggcgt tcacgccgca tccggcataa
     4321 acaaagcgca ctatgtaaat catttgaggc ggcgcaattg cgccgcctcc catcaggcag
     4381 gaattttgtc aatcttagga atgcgtgacc acacgcggtg tgctgccatc agcgttagca
     4441 gttcatccat aaaactaccg tcagcgctgt cagcttccac aatcccttct tcaccctggt
     4501 cagcgacctt gattgttgct ttaaacttgc gcgcgtcacc cgccagcgca atcggtttaa
     4561 ggtgtttgta ggcttccatc aggtagtagt tggcatcgcc gttgtcagcg atatccgcga
     4621 tattgccgca agggacaatg accgcatcga ccgtcagcga aggtgcaccg gcaaaggtag
     4681 cggctatagg caacaccgta ccgtcatccg cagtcacttc acccattcgg gagtagagca
     4741 gtttggcatg aacgcctttg gccttcagcg ccttgagaat ggccagaagg tctgccgatc
     4801 tcacttcatc attaagtaaa atcgctacca cgcgaccttt cacatcaccg tcaggaatgg
     4861 cgtacaaact taaggatgga tcctttttca gaccgttgac gtccggaggt ggggtgatat
     4921 tcagctggtc gtcagtcagt tcgataccga gatttttcgc caccgcctgg gccagagtga
     4981 gatcaatatg cgccagctgg tcaacaacgc gctcacgaat atacggacga acgactttgc
     5041 ttaactcaaa actgaaacca tcgacaatat ggcgctgctc aaatggcgtc tgacttagcc
     5101 agaacagacg cggatgggaa taatattcgc caaacgatgg gctgcgctcg cgaactttat
     5161 tgccttccac gcgctcctgg tatgattcaa aaccgccgcg tttcggcccc ggcggtgttt
     5221 cgcgcggcca gttatcgtta atcgagttcg gttcgtaatt cgccgggtta gtgtcgatcc
     5281 ccatgcgatg catgccgtca cgctggaaat tatggtaagg gcaggtcgga cggttaatcg
     5341 gaatctcatg gaaattcggc ccaccaagac gactgatttg tgtatcggta taggagaaca
     5401 aacgtccctg caacagcgga tcgttggtga agtccagtcc cggcacgata tgcccaggat
     5461 ggaaagccgc ctgttcgttt tcagcaaaga agttatccgg gttgcgattg agcaccattt
     5521 tgccgacacg ctgaacgggc accagttctt ccgggataag tttggttgga tcgagaagat
     5581 cgaagtcgaa cttgaattca tcttcttcag gaatcaactg gaagcccagt tcgtattccg
     5641 gaaaatcgcc tgcttcaatg gcttcccaca actcgcggcg gtggaagtcc gggtcacgtc
     5701 cggtgagttt ttgtgcttca tcccaaacga gtgaggcttt acctgccagt ggtttccagt
     5761 ggaaacgtac aaacgttgcc ttcccttcgg cattaatcag gcggaaggtg tgaataccga
     5821 agccttccat ggtgcggtaa ctgcggggga tgccgcgatc cgacatcgcc cacatcacgt
     5881 tgtgcagagt ttcaggttgc agagaaacat aatcccagaa agtatcgtgg gcactttgcc
     5941 cttgtggaat tgcccagtgc ggttctggtt ttaccgcatg aacaaaatcg gggaatttat
     6001 gcgcatcctg gataaagaag attggcgtgt tattgccaac gaggtcaaaa ataccctctt
     6061 cggtatagaa cttggtggca aagccacgga tatcacgcac ggtatcagca gagccagcac
     6121 caccctgaac ggtagagaaa cgtacaaata ctggggtgat tttgttcgga tctgagagga
     6181 aatccgcttt ggtaatatcg cttaagcttt tatatggctg gaaataaccg tgagcggctg
     6241 atccgcgtgc atgaacaata cgttccggaa tgcgctcatg gtcaaagtgg gtgattttct
     6301 cgcgcagaat aaaatcttcc agcagcgttg gaccacggct accggcacgc agtgagtttt
     6361 gatcgtcggc gatgcgcacg ccctgattag tggtcagcgc ataattttca ctgcctttgc
     6421 gtacgtcttc cagagaatta agtttttcgt tacgcgtatc aggggctttc aggctccctg
     6481 gggcggtagg ttgtgcacct ggcggtgttg gttcagccgc tggacgatga gagccgtcct
     6541 caggtgccag tgagtccatc cccggtttcg cttcgctgga atcgtgtagt ggtgactggt
     6601 gctgatgtgg gttcttttcg ttatgttgcg acattgaact cgtctcctta atttattact
     6661 gaaagggccg ctgttttggt caaaaaactc tctaactata gaccaccagc cagatcccgc
     6721 ttcggagact attctggaaa cggtgttatg aaatacacgc aaagcgggac gtacaggggc
     6781 taaatcggct aaactacagt ttttctgatt tttaatatgc gtttagtgaa gccagtaatg
     6841 aagaaaccgc tccgccagca aaaccgccag attattagct atgtcccacg cacggaaccc
     6901 gcgccgccag aacatgcgat aaagatggat tcgtttcgtg atgtctggat gctgcgtggc
     6961 aaatatgttg cgtttgtact gatgggagag tcatttctgc gctcaccggc gtttactgtg
     7021 cctgaatctg cccaacgttg ggcaaatcag atccgccagg aaggggaagt gactgagtaa
     7081 ggattacata cctcccggtt ttgccgggag ttacatgacc tgaaattctg tatttcgttg
     7141 ctacattcat tcaaaccacc tgtaacaaaa agctgccttt gacgatctat tgctataaat
     7201 aagtgatctt ttttctttaa ttgt
//
LOCUS       DBBGLQ010000165         7168 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.165, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000165 DBBGLQ010000000
VERSION     DBBGLQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.165"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(44..367)
                     /locus_tag="ACIYSB_004909"
     CDS             complement(44..367)
                     /locus_tag="ACIYSB_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGC5431772.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PSSTLSHHISALVSVGLVTQNRESRTLMCVSQYEILEAIIEFLREECCVNSKTDVAEH
                     AGKNG"
     gene            473..1606
                     /locus_tag="ACIYSB_004910"
     CDS             473..1606
                     /locus_tag="ACIYSB_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGC5431773.1"
                     /translation="MNSWISMLQDAAEMFLFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQLMGAKKGKGYLLASLLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIVLDLLGFERHIVDHKNSASSGCTTKC
                     DDSEASVKTSAGSSCCSTSSTQLPLKTSCCTSSANPIINLRTVKNEQNVSACCSSISS
                     EQPAVSCCSSENLGNVNLTTNTTSGPIKLAMKDALQQFKDVLPYLLLSVLIGSFIYGF
                     IPSEWIAAHAGADNPLAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIG
                     SAGASLTEVILLKSMFRMPMIVAFLTVILGMAILMGYLTQLLF"
     gene            complement(1734..2138)
                     /locus_tag="ACIYSB_004911"
                     /pseudo
     CDS             complement(1734..2138)
                     /locus_tag="ACIYSB_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374687.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2306..5293
                     /locus_tag="ACIYSB_004912"
     CDS             2306..5293
                     /locus_tag="ACIYSB_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HGC5431774.1"
                     /translation="MARRQILSLSERESLLALPDDELTLTRMAYFSEHDLALISAHRK
                     PASRFGFAVLLCYLKNVGFAPDKKISPSDALLKHIASRLKLTGDLWPAYLSGRDTTRR
                     EHLTELYRYLGVKAFTGKIQQDCITHLLSMATRTDKGILLAEELLVYLRQNNVIIPAI
                     DVVERTCAEVMAGGDKIVFQTLNAPLTPAHRDALDHLLESSDNQPSRLTWLLQPPGKI
                     NGKNVLQHLDRLSSIESLALPEGIDRTIHQNRLLKLAREGRKMSSRDLTRFSAARRHA
                     ILVCVLEEARATLTDEVIELHERMLNSLFSKAKRTQAERLQQTGKLIQSKLRQYIDVG
                     QALSDARDSGGDPWLAIENILPWAEFVASLDETRHLARKNNFDPLHIITEKYSTLRKY
                     APRMLSALQLVATPAAQPLADALVVIKDMYRKQSRKVPAAAPLEFVPESWRKVVITPV
                     GIDRQYYEFCALSELKGALRSGDIWVKGSRRYKNFDDYLIPEKDFDKLTPALPLPVSA
                     DYHEYITSRMTLLQSRLEEVNAMAALGELPDVEISDRGVKVSPLDNCVPAQVSPLAEL
                     VYSMLPRPKITEILDEVNSWTTFTRHFSHIKNDITRPDTRLLLTTILADGINLGLTKM
                     AEACPGSTKSSLEDIQAWYIRDETYSAALAELVNAQGKRPLAAFWGDGTTSSSDGQNF
                     RTGSSGRYAGQVNPKYGQEPGRQFYTHISDQYSPFYTCIISRVRDSTHVLDGLLYHES
                     DLEIREHYTDTAGFTDHVFALMHLLGFAFCPRIRDLHDKKLFIKGKAEKYPALQSLIS
                     TTSLNLKEIEIHWREVLRLATSIKQGTVTASLMLKKLASYPKQNGLAKALREIGRIER
                     TLFMLDWFRDPALRRRVQAGLNKGEARNALARAVFMHRLGEIRDRKPENQSYRASGLT
                     LLTAAISLWNTVYMERAVDALKRKGVKINEQLLSHLSPLGWEHINLTGDYIWKSNRIP
                     ASGKFRRLRPAKVERSKNSLNVQ"
     gene            complement(5267..6055)
                     /locus_tag="ACIYSB_004913"
     CDS             complement(5267..6055)
                     /locus_tag="ACIYSB_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HGC5431775.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGGQFGYRKLLYVKAVF"
     gene            complement(6055..6858)
                     /locus_tag="ACIYSB_004914"
                     /note="aph(3'')-Ib"
     CDS             complement(6055..6858)
                     /locus_tag="ACIYSB_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HGC5431776.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1810 a         1791 c         1762 g         1805 t
ORIGIN      
        1 tgcagctttc gtcaatcatg acagttatga gttttccggt tacttatcca tttttgccag
       61 cgtgttccgc aacatctgtt ttactgttta cacagcactc ttcacgtaag aattcgatga
      121 tagcttccag tatttcgtat tgagagacac acatgagcgt tcgactctcc cggttttgtg
      181 tgacgagccc tactgatacc agggctgaaa tatgatgact taacgttgat gagggaatgc
      241 caagctgctt ttgcaattca cctacaggca aaccttgttc tccagctttt acaagatgtt
      301 tgaatatgaa caaacgggtc gggtgaccca gctcttttag tgctttagct acttcttcca
      361 gttgcatgtg acaaccctct gtctaatgat atttcgataa taccagaaat atcttgacca
      421 gaattatatt tcgattattc tagaaacgta gattttgtca agagggttta gtatgaatag
      481 ttggatttct atgctgcaag atgccgcaga gatgtttttg tttttggccg tcgagctatc
      541 tttgctgttt attgtcatca gtgccggtgt gagcctgata aggcaaaagg tcccggacca
      601 taaaatccag caattgatgg gggcgaaaaa aggaaaaggc tacctgctgg cctcgttgtt
      661 gggagccgtt acccctttct gtagttgctc aacaattcca atgctacgag ggttgttgtc
      721 ggcgaaagca gggtttggtc cgacactcac ttttttgttt gtttcaccgt tgcttaatcc
      781 cattatcgtg gggttaatgt gggttacctt tggctggaaa gtaactttgt tgtacgcgat
      841 catcgctgcc ggtgtctcag tccttgccag tatcgtattg gatttactag gatttgaacg
      901 tcatattgtt gatcacaaaa actcagcatc aagtggttgc accacgaaat gcgatgattc
      961 cgaagcttcg gtaaaaacgt cggcgggttc cagttgttgc agcactagct cgactcaatt
     1021 gcctttaaaa acgagttgct gtacttcatc agcgaacccc ataattaact taaggacggt
     1081 aaagaacgag cagaacgtat cagcgtgttg ttcatcaata tcatctgaac aaccagctgt
     1141 tagctgttgc agcagcgaga atctgggcaa tgtgaatctc acaacgaata ccacctctgg
     1201 cccaataaag ttagctatga aagatgcctt acagcagttt aaggatgttc tgccttatct
     1261 tttgttaagt gtattaatag gttcatttat ttatggcttt atcccttcag agtggattgc
     1321 agctcatgcc ggagcagata atcccttagc cataccgctg agtgcagttg ttggtattcc
     1381 gttgtatatc cgcgcagagg cagtgatacc acttgcatca gtgctgatga ctaaaggaat
     1441 gggcctgggg gcattaatgg ccttgataat cggtagtgct ggagcgagtt tgacagaagt
     1501 aatactgctt aaatcaatgt tcagaatgcc tatgatagtc gcttttctga cagtaatcct
     1561 gggtatggcc atacttatgg ggtatctgac ccaattgctt ttttaataat aatagtaagg
     1621 gtcttctggt ttcatgttga acaaagaaga ccctttagct tctgtaatcc atttaatcag
     1681 tctgaatgaa atagattata aattctgctc tgcatacctg cttaaccaaa gtctcatgat
     1741 tctgattgtc ggtacagtgc ctgaatgtgg catatctggg aggtgctgta ccctgttgca
     1801 tctgccgttt ctttcttgac ctgccggtag taaagtacct tttgatgccg ttcctgagcg
     1861 acctgttttc cctgatattt acccagcgta tgtgcgagtt caactccctg cttttggcgc
     1921 tggcggtcaa tttgctcgac cagcaaaata tcattgcggt gactgtccat cagtaagcga
     1981 cccagttccg gtcggtcaag ttttgtaccg ctgatattct cccggtagta actggctatt
     2041 ttatgccccc gctcctaaac aaactgctcc agcatctctt ttgcccgatc ggtgaactga
     2101 tcttccgtct atgcccttaa ataagtgctg atgaacattt tttcaccgca ttaccgcatt
     2161 taggttattg catttaatct atcgcatata ggctatgtcg ttcattgtgt gccgtcacgt
     2221 ttagattatg gtgtcagggt atacccttat gcgataacca tttttgttaa aaataacgca
     2281 tcgaataatt tatgaggaaa actatatggc aaggaggcag attctttctc tgtcggaaag
     2341 agaatcatta ctggcattac cggacgatga gttaacgctg acccgaatgg cctatttcag
     2401 tgagcatgat ctggcgctta tcagtgctca ccgtaaaccc gccagccgtt ttggttttgc
     2461 cgtccttctt tgctatctga aaaatgtcgg ctttgccccg gataaaaaaa tctctccctc
     2521 tgatgcgctg ctgaagcata tcgccagcag gctaaagctt accggggatc tctggcctgc
     2581 ttacctttcc ggccgggata cgacccggcg tgagcattta accgaactgt accgctatct
     2641 tggcgtaaag gcgttcaccg gcaaaataca gcaggactgt attacgcacc tgttatcgat
     2701 ggcgacgcgc accgataaag gcattctcct agccgaagag ttacttgtct atctccggca
     2761 gaacaacgtg ataatccccg cgattgatgt cgtggagcgt acctgtgctg aagtcatggc
     2821 aggcggcgat aaaatcgtat ttcagaccct gaatgccccg ttaactccgg ctcacaggga
     2881 tgccctggat catttacttg agtcatcaga taatcagcct tccaggctga catggcttct
     2941 gcaaccgccg ggtaaaatca atggtaagaa cgtgctgcaa catcttgatc ggctcagcag
     3001 cattgaatcg ctggcattac ctgaaggtat agatcgtacc attcaccaga accggttgct
     3061 gaaactggcg cgggaaggcc gaaagatgag tagccgggat ttgacccgat tttcagctgc
     3121 ccggcgtcat gcgatcctgg tgtgcgtgct ggaagaagcc agagccacac tgacagacga
     3181 agtgattgag ctgcatgagc gaatgctgaa tagtcttttc agcaaagcca aacgaacaca
     3241 ggctgagcgc cttcagcaga ccggaaagct gatccagtca aaactgaggc agtacatcga
     3301 tgtcggtcag gcgctgtctg atgcccgtga ttccggtggc gatccctggc tcgcaataga
     3361 aaacatactc ccatgggctg aatttgtcgc cagtctggat gaaacacgcc atctcgccag
     3421 aaaaaataat tttgacccgc tgcatattat taccgagaaa tacagcacat tacggaaata
     3481 tgccccacgc atgttgtccg ctttacagtt agtcgcaacc ccggcagcgc aacctctggc
     3541 tgatgcactg gttgtgatca aggatatgta ccggaagcag tcacgtaaag ttccggcggc
     3601 agcgccgctt gagtttgtcc ctgaaagctg gcgcaaggtg gtgattacac ctgtgggtat
     3661 tgaccggcag tactatgagt tttgtgcact cagcgagctt aagggggcgt tgcgctccgg
     3721 ggatatctgg gttaaaggct cgcgccgcta caaaaatttt gatgattacc tcatacccga
     3781 gaaggacttt gacaaactca caccggcact gccactgccc gtatctgctg attatcatga
     3841 gtacattacc agccgtatga ccctgcttca gtccagactt gaagaggtca atgccatggc
     3901 tgcccttggt gaactgcccg atgtggaaat atccgacagg ggcgtaaaag tctctccgct
     3961 ggataactgc gttccggcgc aggtttcacc gctggcagag ctggtttaca gcatgctacc
     4021 gcgacctaaa atcacggaaa ttctcgatga ggtaaacagc tggacgacgt ttacccgaca
     4081 tttttcgcat ataaaaaatg acattacccg tcccgatacc cgcttgctcc tcaccaccat
     4141 tcttgcggat ggcatcaatc ttggcctgac caaaatggca gaagcctgcc ccgggagcac
     4201 caaatcatca ctcgaagata ttcaggcatg gtacatccgt gacgaaacct attcagccgc
     4261 tcttgcagag ctggtaaatg cgcaggggaa aagaccgttg gcggcatttt ggggagacgg
     4321 aacaacgtca tcatcagatg gacaaaattt cagaaccggc agctcaggcc gctacgcagg
     4381 gcaggttaac ccgaaatatg ggcaagagcc tggacgtcag ttttataccc atatttcgga
     4441 tcaatacagc ccgttttaca cctgcataat cagtcgggtc agggattcaa cccatgttct
     4501 cgatggcttg ctgtaccacg aaagcgacct ggaaatcagg gaacattaca ccgataccgc
     4561 tggtttcacc gatcatgtct ttgccctgat gcatctgttg ggatttgcgt tctgtccgcg
     4621 aatcagggat ctccacgaca aaaaactgtt tatcaaaggg aaagcagaga aatacccggc
     4681 gcttcagtca ctgatatcaa cgaccagcct gaatctgaaa gagatcgaaa tccactggcg
     4741 tgaagtgctg cgtctggcaa cctcgataaa gcagggaacc gtgacggcat ccctgatgct
     4801 gaaaaagctt gccagctatc ccaagcaaaa cggacttgcc aaagcactga gagaaattgg
     4861 ccgcattgag cgaaccctgt ttatgctcga ctggttccgc gacccggctc tgcgtcggcg
     4921 ggtacaggcg gggctgaata aaggcgaagc ccgtaacgcg ctggcgcgtg cggtatttat
     4981 gcaccgactg ggagaaatca gggacagaaa accggaaaat cagagctatc gcgccagtgg
     5041 actgacgctg ctgacggctg ccatatccct gtggaatacc gtctatatgg aaagagcggt
     5101 cgatgccctg aagcgtaaag gggtgaagat caacgagcaa ctgctgtcgc atttgtcgcc
     5161 gttgggctgg gaacacatca atctgacagg tgattatatc tggaaaagca accggatacc
     5221 ggcttctggt aagtttcgtc ggctaagacc agctaaagtc gaaaggtcaa aaaacagcct
     5281 taacgtacaa taattttcga tatccaaact gacccccata agcgtacgcc tggtcgagca
     5341 ggcgacgcgg atcgacgtcc agcgcacgag agaatgcgtc cgccatctgt gcaatgcgtc
     5401 taggatcgag acaaaggtcg tctctgtcag ccggatcgta gaacatattg gcggcgccaa
     5461 agcccacttc accgaccaga ccgacgggat ctatcaccag ccagccgcga ctggagaaca
     5521 tgatgttttc atgatgcaga tcgccatgta gcccacgcag ttccgaggca ttgctcatca
     5581 tttgatcggc tataatcgcc gcgtggacgt agtcagtttg acaacctgcg ttttgatcat
     5641 cgcgcgcccg ctgaaacaaa gctgcaaagc gatcccggat cgggagaagg gcagaaggca
     5701 ggggttcctc agatgcggca tacagcttcg ccattagttc cgctgcaatt tcggtcgcct
     5761 ggtagtcgcc gtgctcggca acgatgtgag agagcattcg ctccccggca tattcgagca
     5821 acatcagatt gttctcacga ccgagcaacc ggactgctcc cctcccattg cgccatacca
     5881 gatagtcggc cccgcgcagt tcatcagcaa tgtcttctat aggtttcaat cccttgacga
     5941 ttgcaggagt cccgtctggc aatgaaactt tccaaacgag gctggaaaag gtgtccgcaa
     6001 tgagaacagg ttgcgaaacg tgccaatgag caggaaaaac aggcggcatg aacatcaacc
     6061 ccaagtcaga gggtccaatc gcagatagaa ggcaaggcgt tcgcggtcgg gggcttcgat
     6121 ccccaataca ttgaatagga cagcgaaggc gcgctctgct tcatctggcg ctgcccagtt
     6181 ctcttcggcg ttagcaatca tgagtgccaa atcggcatag cgatctgctg ttccgagccg
     6241 cccaaggtcg atcagacccg tgcattgaag agttttaggg tccaccatga agttcggcat
     6301 gcagggatca ccatggcaaa caaccatatc ggtgcgctct tggtcgagcc gcaccggtag
     6361 ctctcgttcg acacgagcca aaagatcgag ctgcggcgta ctcttgtcct cgtccggtaa
     6421 gaagtcggga ttgacggcat tgcgggacac cacatcaacg gcgcgtccga acattcgcga
     6481 cagcctgcgc tcaaacggac attgatcaac cgataggctg tgaacagcgc caagttgctg
     6541 ccccattgac ggccacgctt tgagcaaatc cgctccagac agatcagccg ccggtactcc
     6601 cggaattgcc gttatcacca agcatgcacc ctcctgttcc tcctgccagt tgatcacctc
     6661 ggggcaagcc acacctcgac ctttgagcca aatgaggcgg tcacgctctc cagcgagctc
     6721 accgcggcgg gaagcaggtg cgattttcgc gaaggcatgc ccgtcaccac gtcgaaaaac
     6781 aaaatcacca gattctccgc ctctgacagg caaccagtca gaatgcgatt caccaaaaaa
     6841 aatattagtt cgattcaatg gaggttcctt cagttttctg atgaaggggt ccgcttggaa
     6901 aacggaaaat atcccacgct aagactgttt gctgtacaaa ttgatgtcga cagcgcgtaa
     6961 tgaccgtagt cacaggaaca gctaatggta ttgaaaattg acccgtaact gaaacagcat
     7021 cgacccagca gcgatgatgt gaaccttgaa gaactttggg tgcagcctct tatagcagca
     7081 gtttttgctg ttgttccctt aataagatat gccctgtaaa taccctcatg gcttcattta
     7141 taatactatt attagcttac tacgactc
//
LOCUS       DBBGLQ010000166         7130 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.166, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000166 DBBGLQ010000000
VERSION     DBBGLQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.166"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..1150)
                     /gene="trbL"
                     /locus_tag="ACIYSB_004915"
     CDS             complement(<1..1150)
                     /gene="trbL"
                     /locus_tag="ACIYSB_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HGC5431777.1"
                     /translation="MRKKIALGGLMMVATMVLAEPAMAQELSSSGVMNDVLKRFHDAA
                     ATWGPAIESAASRLFWTLVVISMVWTFGMMALRKADIGEFFAEFVRFTIFTGFFWWLL
                     TNANQGMNIAGTIVQSLQTLGAQAGGLSNSNLGPSSILDLGFELYNRTVQATSELGWR
                     QMATALVMELMALAVLFVLALIAVNLLLLLASAWILLYAGVFFLGFGGSRWTSDMAIN
                     YYKTVLGLAAQLMAMVLLVAIGKEFINHYYTQISENMASQELAVMLVISVILLFLVNK
                     VPPMISGLVSGGGIGAAGGIGNFGAGAAVGAAVTAASMATGGAALAGKAVMGAAAGAA
                     GGASALQAAFQKASASMETGGDMSSMGSVVSSGGNGGGEAGTAGSSPFA"
     gene            complement(1161..1397)
                     /gene="trbK"
                     /locus_tag="ACIYSB_004916"
     CDS             complement(1161..1397)
                     /gene="trbK"
                     /locus_tag="ACIYSB_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009292 - horizontal gene transfer
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion lipoprotein TrbK"
                     /protein_id="HGC5431778.1"
                     /translation="MKKIFFTTAATLLLLAGCEQERMPEPNAATCAPDAFQAALNEMR
                     SEANREAFTEECRAFQKAKQMRQWEFKPSPKDDY"
     gene            complement(1410..2189)
                     /gene="trbJ"
                     /locus_tag="ACIYSB_004917"
     CDS             complement(1410..2189)
                     /gene="trbJ"
                     /locus_tag="ACIYSB_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HGC5431779.1"
                     /translation="MKSKILAAKKTALAVALATGFITTTTAPVQAGIPVIDGGNLAQN
                     IMTAIESVAQTLKQIEQYQTQLQQYENQLQNTMAPAAYIWDQAQTTINRLIAAQNTLA
                     YYENQLGSLDRYLAKFQDVAYYRSSPCFNGSGGCTPAEKAAMEENRRLASESQKKAND
                     ALFQTVADQQKALKDDARTLERLQGAAQGATGQLQAIGYANQLASQQANQLLQIRTML
                     TAQHNAEAARIAAELDAEARGDARAEQMRTWTFRPSPADNY"
     gene            complement(2330..2518)
                     /locus_tag="ACIYSB_004918"
     CDS             complement(2330..2518)
                     /locus_tag="ACIYSB_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stabilization protein"
                     /protein_id="HGC5431780.1"
                     /translation="MANVLIRNLPAEVHRALKVRAALNDRSTDAEIREILTAAVQPLE
                     LEEIGREVGLSESDKKTE"
     gene            complement(3551..4432)
                     /gene="repC"
                     /locus_tag="ACIYSB_004919"
     CDS             complement(3551..4432)
                     /gene="repC"
                     /locus_tag="ACIYSB_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HGC5431781.1"
                     /translation="MKKPKHDLTHVRHDPAHCLAPGLFRSLKRGDRKRCKLDVTYTFG
                     EDESMRFVGFEPLGADDMRLLQGIVALGGPNGILLTPEPTSETGRQLRLFLEPRFEAI
                     EQDGLVVRESLTKLLSETGMTDSGDNIKALKASLLRMSNVTILVTKGRRQAAFHLMSH
                     AFDETDGRLWVALNPRIAEAILGHRPYARIDMAEVRVLQTDPARLMHQRLCGWIDPGK
                     SGRVELDTLCGYVWPDEANAEAMKKRRQTARKALAELAAVGWVVNEYAKGKWEIKRPG
                     PTATAPVYRRNVPLLPS"
     gene            complement(4419..5246)
                     /locus_tag="ACIYSB_004920"
     CDS             complement(4419..5246)
                     /locus_tag="ACIYSB_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGC5431782.1"
                     /translation="MALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLAL
                     QLAAQIAGGPDLLEIGEFPTGQVVYLPAEDPPAAIHHRLHALGAHLSAAERQAVADGL
                     LIEPLIGKCPNIMAASWFDALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHME
                     AIAADTGCSIVFLHHASKSAAMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEI
                     LGVDDCQRGYFVRFGVSKANYGAPFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA"
     gene            complement(5251..5472)
                     /locus_tag="ACIYSB_004921"
     CDS             complement(5251..5472)
                     /locus_tag="ACIYSB_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5431783.1"
                     /translation="MQNIKTLINRKKLLEMIPLSTRTIYNLEQRGDFPRRIALTSRNV
                     AWDLSEVEEWIEARKSSGDQAARPGPIEG"
     gene            complement(5620..6822)
                     /locus_tag="ACIYSB_004922"
     CDS             complement(5620..6822)
                     /locus_tag="ACIYSB_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5431784.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVAVTPAGSISFRYNYSIN
                     GRQETITFGRYGVGGITLAEARELLGDAKKMVAAGKSPAKEKARDKARVKDAETFGAW
                     AEKWLRGYQMADSTRDMRRSVYERELKPKFSNQKLVEITHEDLRALADAIVERGAPAT
                     AVHVREIVLQVFRWAIERGQKVENPAELVRPTSIARFEPRDRALTPEEIGLMYQYMER
                     VGTSPTNRAAAKLLLLTMVRKSELTNATWSEINFSEALWTIPKERMKRRNPHLVFLSQ
                     QALDIFIAMKTFAGGSDFVLPSRYDSDAPMSAATLNQVLTLTYKAAQKDGKSLTKFGP
                     HDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRAAMLQDWADMIDE
                     WTSGGSKG"
BASE COUNT         1591 a         2122 c         1795 g         1622 t
ORIGIN      
        1 gggcgaatgg gctactgcca gcagtacccg cttcaccacc accgtttccg ccactgctga
       61 caactgaccc catgctggac atgtcaccgc cggtttccat actcgctgat gctttctgaa
      121 aagccgcttg gagtgcactt gcacctccgg ctgcaccggc tgcggcaccc ataaccgctt
      181 tacctgccag ggcagcgccg ccagttgcca tactggccgc cgtcaccgcc gccccaactg
      241 ccgcacccgc accgaagttt ccaattccac cggctgcgcc aataccacca ccagacacaa
      301 gaccagaaat catcggcgga actttgttga ccaagaaaag caagatgacc gatataacca
      361 acatcacggc cagttcctgg gatgccatgt tctcgctgat ttgcgtgtag tagtgattga
      421 tgaactcttt gccaatcgca accagaagca ccattgccat aagctgtgcg gccaggccca
      481 gcacggtctt gtagtaattg atcgccatgt cggaagtcca acgacttcca ccaaagccaa
      541 ggaagaacac accggcatac aacagaatcc aggctgatgc gagcaacagc aacaggttga
      601 ccgcaatcaa cgccaggaca aacaaaacag ccagggccat tagctccatg accagagcag
      661 ttgccatctg ccgccatccc agctccgagg tggcctgtac tgtgcggttg tataactcga
      721 aaccaagatc aaggatgctg gagggcccaa gattgctatt ggaaagtccc cccgcctgcg
      781 cgcccagagt ttgcaatgac tgaacaatcg taccggcgat attcatgccc tggttcgcat
      841 tcgtcagcaa ccaccagaaa aagccggtga agatcgtgaa gcgaacgaac tccgcgaaga
      901 actcgccaat gtcggccttg cgcaaagcca tcatgccgaa tgtccagacc atcgaaatca
      961 caaccagcgt ccagaacaaa cgcgatgcag cggactcaat agctggcccc catgttgcgg
     1021 cagcatcatg aaaacgcttg agcacatcat tcataacacc gctgctactt agctcctgcg
     1081 ccattgcagg ttcagccagc accattgtgg cgaccatcat caagccacct agagcaattt
     1141 tctttctcat ggcgaccacc tcaataatca tctttagggc tgggcttgaa ctcccactga
     1201 cgcatctgct tggccttctg gaatgctctg cattcctcgg taaaggcctc tcggttcgct
     1261 tcgctgcgca tttcattcag tgctgcctgg aacgcatcgg gggcacacgt cgccgcattt
     1321 ggttcgggca tcctttcctg ttcacatcca gccaacaaca gcaacgtagc cgcggtagta
     1381 aaaaagattt ttttcatctc accgcctccc tagtagttgt ctgccggact cgggcgaaac
     1441 gtccaggtac gcatttgctc ggccctggca tcacctcgcg cctcggcatc gagttctgct
     1501 gcaatccggg ctgcctccgc gttgtgttgg gcggtcaaca tggtgcgaat ctgcaagagc
     1561 tgattggcct gctggctggc gagctggttg gcgtagccga tggcctgtag ctggccagtt
     1621 gcaccctggg ccgcgccttg cagccgctcc agggtacggg cgtcatcctt caaagccttt
     1681 tgctggtcgg caacggtctg gaacagggca tcgttggcct tcttttgcga ctctgacgcc
     1741 aggcggcggt tctcttccat cgctgccttt tcggccggcg tgcatccgcc gctgccgttg
     1801 aagcatggcg agctgcggta ataggccacg tcctgaaact tggccaggta acgatccagg
     1861 ctgcctagct ggttctcgta ataggccagc gtgttttgcg cggcaatcag ccggttgatc
     1921 gtagtctgcg cctgatccca gatatacgcg gctggggcca tcgtgttctg gagctgattt
     1981 tcgtactgct gcaactgggt ctggtactgc tcaatctgct tgagcgtctg cgccaccgac
     2041 tcgatggccg tcatgatgtt ctgggccagg ttgccgccgt cgatgacggg gataccagct
     2101 tgcacagggg cggtggtagt ggtgataaag ccggttgcga gtgcaacggc tagagcggtt
     2161 tttttagccg ctaaaatttt ggacttcatg gtcgtactcc gtcgatgaaa agaaggcatt
     2221 gccttgacca agcgttgtgc ttgttctcgg caacgtttca tcgaagagct acgccactag
     2281 ctgatcggta ttgcccttgg cgctgaggcc cggacttaca aacgtaaaac tactctgttt
     2341 ttttatcgct ttcggacaag ccaacttccc ggccaatctc ttcaagctct agcggctgta
     2401 ctgccgccgt cagtatttcg cgtatttccg cgtcggtgct cctatcgttg agcgctgctc
     2461 gaactttcaa cgcacggtgc acctcggcgg gtaaattcct gataagcaca tttgccatat
     2521 caataccctt ttggtgtacc cgacaaccat gctatcaaag atattaaaaa tagcaagacc
     2581 tatactcttg caaccggcgg tagccgatca gccccgcagg gcaggattcc cgttgagtgc
     2641 ccccggcgcg aataagggac agtgaagata gataaccggc ttgccggtta gctaacttca
     2701 cccatcctgc ccgcatttca cccactattg aaaatctagc gcatatcgaa cgtaaagcgt
     2761 tttatcgtca aacaaacacc gattcttggc aacataaccg taatcgtcac caaatcggcc
     2821 tgcaatcgac atagtcgctt caaaatcgct gctcaatcgg ctttaaatcg gaacctaaaa
     2881 tgccaacaat cgacacaaac cattcgcccc gatggggcga gttggacgcc aaaaaagaag
     2941 ggcataaccc gcccctcaag tgtcaatgaa cacaagaaat ttgcctcccc tcccgtcata
     3001 ccactaaggc gacgtattga tacttgaggg gcaacctttc ctgatgttga caccttcacg
     3061 caggttactg gttatgggcg tccaacacac acggctcatt cctgcttggt aataataaga
     3121 ataataatct tcttattatt cttaccgaac gaatgggcac tgcttagcca tttcacgttt
     3181 cgagccaagg tttcagtaca gataactaca agtgcacaca gcggccctcc agcaccgtta
     3241 gacgcagatt ctgcttcagc tcatggaata agggactccg ggttgttttc ccctctcaca
     3301 aggcgtttca gcggccactg aagccgcctt ctcttcttgg cgttaaacca tcggcctatg
     3361 gccgattgat aaagcccgcc agggctttat tggcaaggtc agaacgcgcc ctcaaggcgc
     3421 gagctgcacc gcgcatgatg gattatcttt agataatctt ggatggattt cccaaactgc
     3481 ccgcaaagca gcgcaccgtc tgggttttcc aaattgggac ggggaacgtt acgacggcag
     3541 aaggggagcg ttacgacggt aacaagggaa cgttacgacg gtaaacgggt gcagttgccg
     3601 tggggccagg cctcttgatc tcccattttc ccttggcgta ttcgttcact acccaaccca
     3661 cggcggcaag ttcggccagt gccttccggg cagtctgacg gcgttttttc atagcttcgg
     3721 cattggcttc atctggccag acatagccgc aaagcgtgtc cagttccacg cgcccggatt
     3781 tgccggggtc gatccagccg catagccgtt ggtgcatcag ccgtgccgga tcagtctgta
     3841 gcacccgcac ttccgccatg tcgatacggg catatggacg atgccccagg atcgcttcgg
     3901 caatacgcgg attcagggca acccatagcc tgccgtccgt ctcgtcaaaa gcatgactca
     3961 tcaggtggaa cgcggcttgc cgccgtccct tcgtcacaag gatggtgacg ttcgacatgc
     4021 gcagcaggct ggctttgagc gccttgatgt tgtcgccgct atccgtcatg cccgtttctg
     4081 agagcagttt ggtcaggctc tcacgaacca ccaagccgtc ttgctcaatg gcttcgaaac
     4141 ggggttcaag gaatagccgt agctgtcgcc ccgtctcact ggtcggttcc ggggttagca
     4201 agatgccgtt cgggccgcca agggccacga tgccttgcaa aagacgcata tcatcggccc
     4261 cgagaggttc gaatccgacg aaacgcatgg attcgtcctc gccaaaggtg taggtcacgt
     4321 ccagcttgca gcgtttgcga tcgccacgct tgaggctgcg gaacaggcca ggtgccaaac
     4381 agtgcgcggg atcatgtcgg acgtgggtca ggtcatgctt aggcttcttc acgctgtctc
     4441 cttttcacct tgcactgccg ctccagtaca gcaggcttca acacgccgcc gtcgtgccgt
     4501 ctaaaccaga gttcttgaaa aggtgcgcca tagttggcct tgctgacgcc aaagcggaca
     4561 aaatacccac gctgacaatc atcgaccccc agtatctcgg cctcgccttg cgtcatgccg
     4621 gataggtacg attgccaacg gatgttatca accagtacgg acgatccacg actggcctgc
     4681 tgttgatcgc ccgaccccat catggctgcg cttttactcg cgtggtgcag aaacacaatg
     4741 gagcagcctg tatcagcggc tatggcctcc atgtgcccaa caacctgcgc catcggcccg
     4801 ctagcgttct cctcctcaat gtggaagcgc cgcaaagtgt ccaagatcat caagcgacga
     4861 ccttcagcgg ctcgtttgag agcatcgaac cagctagcag ccatgatgtt tgggcattta
     4921 ccgatcaagg gttcaatgag caaaccatca gccacggctt gccgttccgc tgcgctgagg
     4981 tgtgccccaa gggcgtgcag acgatggtga atagcggccg gtggatcttc agcaggcaga
     5041 tagaccactt gcccggtggg aaactcgcct atttcaagta aatcaggccc gcctgcaatc
     5101 tgtgcagcca attgaagggc caacatggat ttaccagccc caccaggcga cacaagagca
     5161 ccaacagtgc cagcaaccat attaggcaga acataatcaa tgggtggcgg caattccgtg
     5221 aaagccgcca taagatcaag agccatataa ctagccctct atagggccag gtcgcgcagc
     5281 ttgatcaccc gatgatttac gtgcctcaat ccactcttcg acctctgaca aatcccaggc
     5341 gacatttcta ctggttaacg cgatacggcg tggaaaatcc cctcgctgct ccaggttata
     5401 aattgttcgt gtcgaaagcg ggatcatctc caggagcttc ttcctgttga tgagggtctt
     5461 tatattttgc atgggtcaat acctctcaaa tgaatagttg ctattcatcg agtagctgcg
     5521 cgaagcagct aatcggtaat tgacctgccc gatgccaatg gcggccttat ttataaattc
     5581 acggcatcaa cttcaatgct atcaaagata gcaaaaacat caacccttac tgcctcccga
     5641 agtccactca tcaatcatat cggcccaatc ctgcaacatc gccgcccgct gctcgcggta
     5701 ctcagccttg ttgtagacag ccctcacgcc cttctgctcg tgcgccaggc acttctcgat
     5761 ccagtcggtg ttgtagccgg cctcatgcaa cagcgtgctg gctgtgcgcc gcaaatcatg
     5821 cggtccgaac ttggtaagtg acttcccatc tttctgcgcc gccttgtaag tcagcgtcag
     5881 cacctggtta agtgtggcag cgctcatcgg cgcatccgag tcgtaccgtg atggcaaaac
     5941 gaagtcggaa ccaccggcaa aggttttcat ggcaatgaaa atatccagag cctgctggga
     6001 cagaaatacc aggtgcgggt tacggcgttt catccgctcc tttggaatcg tccacaacgc
     6061 ttcgctgaaa ttgatctcgc tccaggtcgc attggtcagc tcgctcttgc gcaccatcgt
     6121 cagcaataac agcttggccg ccgcacggtt tgttgggctt gtgcccactc gctccatgta
     6181 ctggtacatc agcccaattt cttctggcgt caacgctcgg tcacgtggct cgaatcgagc
     6241 gatgcttgtt gggcgcacca gttctgccgg gttttcgacc ttctgcccac gctcgatggc
     6301 ccagcgaaat acctgcaaca cgatttcacg cacatgaacg gcggtggccg gtgcgcctcg
     6361 ctcaacaatg gcatcagcca gcgcccgcaa gtcttcgtgg gtgatctcca ccagcttctg
     6421 attgctgaat ttcggcttca gctcacgctc ataaaccgag cggcgcatat cgcgggtgga
     6481 gtcggccatc tggtagccac gcagccactt ctccgcccag gcaccgaacg tctctgcatc
     6541 tttcacccgc gccttgtctc gggctttctc cttggccggc gacttgcccg ccgcaaccat
     6601 cttcttggcg tcacccaaca gctcgcgggc ttcggccagg gtgatgccac cgacaccata
     6661 acgcccaaag gtgatggtct cctgccgacc gttgattgag tagttgtaac ggaacgagat
     6721 ggagccggct ggagtcactg ccacatagag accttcccgg tcattcactt tgtagagttt
     6781 gtccctgggc ttgagattgc gcagcttggt atcggtcagc atgtaccttg tccttcttcg
     6841 tctccgatac catgctgaaa aaatctcgaa tttatcgtat tttttcttac gaaacagtaa
     6901 cttatagaat attaatacca tgaacacaca aaagaacgca gcatggtatc ggcatcaatc
     6961 atggcatcgc caaaccataa taccagcttt aatgccatgc tcaaacggtg cttggcgctt
     7021 cctcccgttg ccagatcgtg ccaaacacaa acaaaaaaaa gcccgcaatt acgcgggctt
     7081 agcggcattt catgccatca ggtgcctggc tgtgccagac gcggaatcat
//
LOCUS       DBBGLQ010000167         7021 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.167, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000167 DBBGLQ010000000
VERSION     DBBGLQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.167"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..135
                     /locus_tag="ACIYSB_004923"
     CDS             <1..135
                     /locus_tag="ACIYSB_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431785.1"
                     /translation="TGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF
                     "
     gene            353..2756
                     /locus_tag="ACIYSB_004924"
                     /pseudo
     CDS             353..2756
                     /locus_tag="ACIYSB_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            2753..3658
                     /locus_tag="ACIYSB_004925"
     CDS             2753..3658
                     /locus_tag="ACIYSB_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGC5431786.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            3655..4725
                     /locus_tag="ACIYSB_004926"
     CDS             3655..4725
                     /locus_tag="ACIYSB_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGC5431787.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            4861..5544
                     /locus_tag="ACIYSB_004927"
     CDS             4861..5544
                     /locus_tag="ACIYSB_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431788.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            5560..5970
                     /locus_tag="ACIYSB_004928"
     CDS             5560..5970
                     /locus_tag="ACIYSB_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431789.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            6191..7012
                     /locus_tag="ACIYSB_004929"
     CDS             6191..7012
                     /locus_tag="ACIYSB_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGC5431790.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLSPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTWAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         1829 a         1691 c         1918 g         1583 t
ORIGIN      
        1 accggtgcgg tggaccggat atttgacacg gcggagagcg tctgcattgc ctctgttgca
       61 cgcacggttc tgcgcgccgt ccgtgactcg gtggtctctg ttgcccgcgc ggtatggaac
      121 tggattttct tctgaacccg gtggtgatgt accaggctgt tgttacagat ttttaactcc
      181 atgaaaacac tatgcaaacg cagccatacc cgggttgtcc ccttgcgttg tgtttctcaa
      241 tttaatagaa tacatcgatc gttactatca tatcgatcgt tataaacgat cgtttgtagt
      301 aaatgacctc ctccgtggtt gtcatgaaaa tgcgtcttag gaaggttaaa taatgcacga
      361 aaagaacatc gccctgcttt gtgatgaagc cgaccgactt ttgcaactga acattaatct
      421 gctccggcaa atggttgagg agccagatgt gttatctgac agtaagaacg aaaacagact
      481 gctttttgat aaacagaaag cactgaaaag aattgaggag ctggagggcg aacaaatcaa
      541 aaccgcccgc agggagatgg tgctggctgt tgtcggcacg atgaaagcag gcaaatcaac
      601 caccatcaac gccattgtgg ggcaggaaat tctgcctaac cgtaaccgcc ccatgacctc
      661 tgtaccgacg ctcatccgcc acgttcccgg aaaaactgag ccggttctcc atctggaaca
      721 tattcagcct gtccgcaatt tattaatcac actgcaggaa aaactcgcca ccccggcagg
      781 acagcaggtc gcacagaccc tgcagcaaac cggggatacc cgcgaactgc tggatattct
      841 gacggatgat ggctggctca aaaatgaata ccacggggag gaggaaatct ttaccggact
      901 ggcatcgtta aacgatctgg ttcgtcttgc tgcggcaatg gggactgaat ttccttttga
      961 tgaatacgca gaagtgcaga aactgccggt gatcgacgtg gaattcagcc atctggtggg
     1021 gatggatgca tgccagggaa cactcacact gctggatacc cccggcccta atgaggccgg
     1081 acaaccgcag atggaagtga tgatgcggga tcaactgcag aaagcctctg cggttctggc
     1141 tgtgatggat tacacccaga tgaactcaaa agcggatgaa gacgtccgta aagagcttaa
     1201 tgccattgct gacgtatcag ccggccgcct gtttgtactg gtcaataaat ttgatgagaa
     1261 agaccgcaat ggcgatgggg cagatgccgt acgccagaaa gttccggcaa tgctgaacag
     1321 cgatgtgctg cccgcctccc gcgtttatcc cggatcctca cgccaggcat acctggctaa
     1381 ccgtgcgctt catgagttac ggaaaaacgg aacccttcct gttgatgaag cctgggtcga
     1441 tgattttatc agaggtgcct tcggttgcat gaaaaaagaa tacgtctgta aagacagtga
     1501 actggcaact gaaggggcga cagacctgtg ggaaggctca cttatcgatc aactgataac
     1561 ggaagtcata cagagctcac attccagagc tgcggcactg gcggttgact ctgccgccgc
     1621 aaaactgatg cagaatgcag aatgcagaaa atatcagtga atacctgttg ttacgccatc
     1681 aggggctaca gcaaagcatt cagtcactgc agtcgcatat caccagcctg cttgcggata
     1741 tccgggaaat cgcggactgt caggagcaga tgaccactga tgtcagaatg gccatggagg
     1801 agatcgatac taaaacccgg gaattactga cgggggtctg cacctcactg gaagaggagc
     1861 tgaatgacta tttcagaagc ggtaaacgca aagaacagca aatgctggag gaagaagacg
     1921 cagaacaacg aaggtctcag tccggattat gggggaaaat ttctcaatgg tccggtatca
     1981 acaaccaggg ccgggaggat tacaggaaac gagattttgc cccggacagc cccgaaataa
     2041 aattcagtga tcgcagggaa gcccttgaac tgatgacgca aatcgaatcg accgtgacca
     2101 gcctgcaccg tgaggctgaa gcacagttcc ggcctgagct ggagaaaatc gtcagcggga
     2161 ttgaaacagg ttttcgtggc acggccctgt acgccacaga aaacattgcc ggtcgcatca
     2221 atgcccgcct ggaggatgag ggctttaccg taaaaatcag ttttccggca gtcagccagt
     2281 tacagacccg gctcgcggta aaaataaatc tgagtgcgct tatggaggaa agaacggaga
     2341 ccgtcacccg tcgccgtcgg cagagtggcg tatggggaac cgtttgtcga tggtttggca
     2401 ccagtgacct gggctgggaa aactatgacg aggatgtgag tcgcagcgtg atcaatatca
     2461 acaaggtcag agaggaagtt atgtcactga cccgggcata tttcggggag ctgcaggcat
     2521 ccattgagca ggatattaac cagcccgtcc gccaggaaat cgatgccttt ttctgcgcat
     2581 tcagggagaa agttgaacaa ctgcgtaaca cgctgattca gagctctgaa gatcataaac
     2641 gcgatcagca ggcgcaggaa cggcttaccg ggcgacttca ggcattaaat gaaagggttc
     2701 ctgagctcat tactgacagt aaggcgctga gggaagagct ggagacaatg ctgtgacttc
     2761 accatttatt caacaaattg ctgataaccg ggtatgtcag gtactcacct gtcttcctga
     2821 aaaatttgtg gttgatttcg caaacggaat cgatgttgca caggagcata tccgcacggc
     2881 cggagagcgc acgttcttcc ggcgtttaaa agaggggctg accggcgaag gcgctgcacg
     2941 gcagaatgcc atcaatgcgt cgcttgcaca gggcgttgag gcgtccctgc gctggctgac
     3001 ggagatgaca acgtcacttg ccaccacaaa ctacgcgatt acccgggtaa acgacagggt
     3061 cagttcactg gtcagtgata cagccaggct ggcgcattat tccgcagata cgcgggagca
     3121 gttactcacc ctggccgatc aggttcacca taaactgaat catcttgaag aaaaactcca
     3181 ccgtgttgac caggttcagc gggcgcagtt acatcttgag cagatattct catggtggag
     3241 cgccgggcga tatgcgtcat tttcccctgc cggacgttgt tatgtggcac ttgaagagct
     3301 tcgctggggc gcgtttggtg atgtgatacg tcagagcgaa acaggccagg ttaaccagct
     3361 actggatatt ctcagacata aagcattaac gcagatggca caggagagtg gcggtagtgc
     3421 aaccgtgcgt ctgaacactc tggactggct tggtggtcag ggccgggagc aggcagataa
     3481 cgagtggcat gacgcgatta actggctggg agactggtgc agtgaagagc agcatccggt
     3541 gatctggtca accacgcagg ctgcagaaca cttaccggtt cgcatgcctc gcctctgctc
     3601 tgcagaacgc ctctctgaaa gtatggtcga tgaaatattt cagaaggggg ctgcatgagt
     3661 acagaaatga aaacggggct ggtgctgtcc ggtggcgggg cggtgggcgc ttatcaggcg
     3721 ggagtggtta aggcactggc agagtgtggt acacagatca gcatggtttc aggggccagc
     3781 attggcgcat tcaatggtgc cattatcgcg gcctctcccg atctgtcaga agctgccgta
     3841 cgcctggagg cgctctggga tcatctgggg aataatcagg tgctgtcggt aaacagattg
     3901 gtttactttt cattgctgaa aaaattgttc caggcaatga acctctgcca gatccccgga
     3961 cgtgcaggag cactgcttac gacgcttctt cgccatatat cgacaatcaa cgggtttgac
     4021 aatctgatgg ctcagccgtt gttgtcagat gagcccctga cagcgctgat ggatcattat
     4081 cttgatactg atgctctggc agacgggcta ccgctgtatg tgtcgctgta ccccacagaa
     4141 gggggcatgc aggatattat tgactgcatt cgtgctgaac tgggtgtcgg aaccacgaaa
     4201 aacgccgttt ttcagcatat ccagagcctg ccccgcggac agcagaaaga ggctctgctt
     4261 gcgtcagccg cgctgcccct gctgttccgt ccccgtgagg ttcaggggac aatgttcggt
     4321 gatggtggta tgggaggatg gcgaaatatg cagggaaata cccctgtgac gcctctggtc
     4381 gatgccggat gcaatatggt gattgtgacg catctgagtg acggttcttt atgggatcgc
     4441 caggcttttc cggacaccac aatccttgag atccgtcccc ggaaaaggct gaaatatgca
     4501 ggtgatggtg gcaacagcgg cggtctgctc agttttacat cggcacatac cgacgcctgg
     4561 cgtcagcagg gctatgaaga cacgatgctg gcgatggagc atatccggaa accgctggca
     4621 gcacgtcagg cactgacccg gtcagaggcg gtattgcaga aaagcctgga tataacggaa
     4681 gaggcagatt tggcactgag aaacgcgatg gcccggatta aataaagacg ccccaaagaa
     4741 acgccaccgc ctgtggtggt gtttttcctg tctggtgtta ttgaaaatat tttcagatat
     4801 atattacctg agagaaagtt ttacccctca cctgtcaatt cactcactgg aaatttaatt
     4861 atgaaattac ctgtaaagtt attaatgagc cttatatctc tggtcagcgt tattgcacgt
     4921 gccggggaat ataaaaatta cagccgggat gaaataaaat actggcgata tacatcatac
     4981 aaggggggga aacttccgga aggtttcact gatgagaaat tttccagcgc catttacaac
     5041 ggaagaatat ttacaatgaa acgtttacat acccttatgt tatttctggc ggttctgttc
     5101 acgggcttta actttaacgc agaagcggct accgtaaaac aagctcttag ttgtaaccca
     5161 gaagcctggg ctgaacaacc tggagcgtgt ccatcaacgt acgagttgta cgaaggtgac
     5221 gctacctaca aagccgctat cgacaaagca ttaaaaccgg tcggactgag cggcatgttc
     5281 ggtaaaggcg gctatatgga tggccctggt ggaggtatca cgccagtaaa cattaacggt
     5341 acagtctggt tccagggcga cggttgcaaa gccaatacct gcggctggga ctttatcgta
     5401 acactctata acccaaaaac ccatgaagtt gttggctacc gttactttgg tttagatgac
     5461 ccggcctacc tggtttggtt cggtgaaatt ggcgtgcatg aattcgcgta tctggtgaaa
     5521 aactacgtag ctgcggttaa ctaatttaag gagtctatca tgaaaacttt tatcaaaact
     5581 ttactcgttg ctgtaactat tctgttctct gtcttcgcta ctgcgaaaca agtaaaactg
     5641 ccaaataaca tcaaatacgt taatactaca gaagcgtttt cctgtaccga aattgacggt
     5701 atgaattgcc agacaaagaa tcagttcaac tataaagata acagctatgt tttcgtgctt
     5761 gaacgtggtg gtgcctggtg ctacgactac actgtctcgg tagttaacct gaaaaccggg
     5821 aaagcacaaa tgattgaata cggggacaat caactgtgct caggtagcaa caaaccgttc
     5881 ttcgaaatca aaaatggcgt accgacggta ggagtcatcg acacatccgg aaaacctgtc
     5941 gttgtagccc aggacaaact taaaatctaa ggagtaaacc atgcgtttct ctgcacatta
     6001 ttaatatcaa aggtaaatac agccccagcc attgttaccg gaaagccgct taatgcgtgg
     6061 ctcgccagtg aaaggcctct cagacaataa accttattca tttcacccgt caggccgcgt
     6121 cttctccggg agacgcggct tttttcattt attgcaccag taaatcttaa ccaccgataa
     6181 ggagcaaagt atgcgattag caagtcgttt tggttatgct gcaaaccaga tacgccgtga
     6241 ccgtccgctg acacatgaag aactgatacg ccatgtaccc agtatttttg gggaagaccg
     6301 gcacacctcc cgcagtgaac ggtatgcgta cattcccacc attaccgtcc tggaaaatct
     6361 gcagcgggaa ggctttcagc cgttcttcgc ctgccagacc cgtgtgcgcg acccgggccg
     6421 ccggggatac acaaaacaca tgctgcgtct gcggcgggcc ggagagataa acggagaaca
     6481 tgtccctgaa attattctgc tcaactctca tgacggtacc tccagctacc agatgctgcc
     6541 gggttacttc aggttcgtct gccagaacgg gtgcgtctgt ggccagtctc tgggggaagt
     6601 gcgtgttcca caccggggaa atgtggtgga cagagtcatt gaaggggctt acgaggtggt
     6661 gggcgtgttt gaccggatag aggagaagcg tgatgccatg cagtcgctgg tcctgtcgcc
     6721 accggcacgc caggcgctgg cacaggcggc actgacttac cgttatggtg acgaacatca
     6781 gcccgtcacc accgccgaca ttctgacgcc gcgacgccgg gaggattacg gtaaggacct
     6841 gtggagcgca tatcagacca tccaggagaa tatgctgaaa ggcgggattt ccggtcgcag
     6901 tgcaaaagga aaacgtatcc atacctgggc cattcacagc attgataccg acattaagct
     6961 caatcgcgca ttgtgggtga tggcagaaac gctgctggag agcctgcgct gatgccgttt
     7021 c
//
LOCUS       DBBGLQ010000168         6887 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.168, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000168 DBBGLQ010000000
VERSION     DBBGLQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.168"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(246..914)
                     /gene="artM"
                     /locus_tag="ACIYSB_004930"
     CDS             complement(246..914)
                     /gene="artM"
                     /locus_tag="ACIYSB_004930"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGC5431791.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(914..1630)
                     /gene="artQ"
                     /locus_tag="ACIYSB_004931"
     CDS             complement(914..1630)
                     /gene="artQ"
                     /locus_tag="ACIYSB_004931"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGC5431792.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1637..2368)
                     /gene="artI"
                     /locus_tag="ACIYSB_004932"
     CDS             complement(1637..2368)
                     /gene="artI"
                     /locus_tag="ACIYSB_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGC5431793.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2386..3114)
                     /gene="artP"
                     /locus_tag="ACIYSB_004933"
     CDS             complement(2386..3114)
                     /gene="artP"
                     /locus_tag="ACIYSB_004933"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGC5431794.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3332..3847)
                     /gene="ybjP"
                     /locus_tag="ACIYSB_004934"
     CDS             complement(3332..3847)
                     /gene="ybjP"
                     /locus_tag="ACIYSB_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGC5431795.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3973..4296
                     /gene="ybjQ"
                     /locus_tag="ACIYSB_004935"
     CDS             3973..4296
                     /gene="ybjQ"
                     /locus_tag="ACIYSB_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGC5431796.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4293..5123
                     /gene="amiD"
                     /locus_tag="ACIYSB_004936"
     CDS             4293..5123
                     /gene="amiD"
                     /locus_tag="ACIYSB_004936"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGC5431797.1"
                     /translation="MRRFFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5120..6133)
                     /gene="ybjS"
                     /locus_tag="ACIYSB_004937"
     CDS             complement(5120..6133)
                     /gene="ybjS"
                     /locus_tag="ACIYSB_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGC5431798.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6232..>6887)
                     /locus_tag="ACIYSB_004938"
     CDS             complement(6232..>6887)
                     /locus_tag="ACIYSB_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGC5431799.1"
                     /translation="ALIQGLKHDLLADDTALRALIPQRLIAFDDAVRSTLKEEEKLVN
                     SSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQIGGKERYFFGNILW
                     QTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTLLFGMKAPGLGRL
                     CFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAKQIARLAEQSTD"
BASE COUNT         1683 a         1803 c         1808 g         1593 t
ORIGIN      
        1 cggcatggga taacaatgcc atcaagtgaa tttatatgca ataaacatga ttaaataatt
       61 taaatgaaat aaaaaagacg gacaacttag tgggttgtcc gtcttcatta taagaattta
      121 tgcactatgt aggccggata aggcgtcccc gccgcatccg gcacaggcac cgtgctgatg
      181 tctgatgcga cgctggcgcg tcttatcaga cctacaaaac cccccggcga atgtacgcag
      241 ccacattaat ttcgccgttc gaatgccagc gctttgcgct cgatcagacg catcatcagc
      301 gtcagcaggc cgttaacgac caggtaaata atccctgccg caccgaacac cattacatcg
      361 taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt aatggtgtat
      421 gccagagagg tacttttgaa taccagcacc acttcgttgg aataagaaga gagcgagcgt
      481 ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc cagggcgcta
      541 caggactgcc actgaccttc cgggatcgca cgaattgcac cgtaaaacag ctgcgtggta
      601 tacgccgcac tattcagcga caacgcaatc agcgcacata accacggttc tgacaacaaa
      661 tgccacagtg ccggatactc ctgcaaagtc ggaaactggc ccggcccgta ataaatcagg
      721 aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg caccagccac
      781 accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc
      841 acaatcagcg aggcaacggt tagcgtcagg ctggtgtgta gccctttcat cagttcgggt
      901 aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt
      961 tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc acaatgtacc
     1021 aggtaaatgg ttcctgggta cgagtagcga tgctttttgt ttgcagcatt aaatcattca
     1081 cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg ttaccgaggc
     1141 caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata gccgatttcg
     1201 acagccccag cgcctgacca gactcccact gacccaccgg caccgctttc aacgcgcccc
     1261 gaagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca ccacaaagga
     1321 acgggctaac gtcgaagttc tcaatgtcca tctgcactgg gatctgcacg aacccaagat
     1381 tgatagtgaa gccatccgaa agcgtcagca gcagctgcga ggagccaaaa tagataaaca
     1441 gcaccaccag aatttctggc aggccacgca gaatggttac cagcgctgaa cctgcccacg
     1501 cgacaggacg ccattttgcc gactcccata ccgcaaagaa catcgccagc gccagcccga
     1561 caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa
     1621 attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg taagtgccat
     1681 ctttcttcac tttttccagc gcagtgttga gtttctgctg cagctcagtg ttgccctgac
     1741 gtaccgcgat gccgaggcca gtgccgaagt aatctttatc ggtcactttg tcgcccaccg
     1801 ccgccagttt cgggttatct ttcagccact cagtgaccac tgcggtgtca ccgaagacgc
     1861 cgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca tacggaacgg
     1921 tagtgatttc cgggtgctta tccataatga atttctggtg tgtcgtcccg ttctgtacgc
     1981 cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga cccacaaaca
     2041 gggcagagtt gtcatagtac ggggtggtaa acagcacctg cttttcacgc tccggagtga
     2101 tatccatgcc cgccatcacg gcttctacgc gacggaattt caggcttggg atcaggctgt
     2161 caaacgcctg gttagagaaa gtgcaggttg catcaatctc tttacacagc gcttgtgcca
     2221 ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac ggaggatagg
     2281 aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta aaacctgcaa
     2341 ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt gagagagata
     2401 gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt gttctacgat
     2461 atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt caacttcgtg
     2521 ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc tgacgatttg
     2581 tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta cctgcggttc
     2641 catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg
     2701 gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg cacgggccag
     2761 cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt tttgctgcac
     2821 ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac gacgcaaatc
     2881 gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg caatgttgag
     2941 cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc ttttacccgc
     3001 gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg tgatatcgaa
     3061 cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac tcattgacac
     3121 tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga cagaataatt
     3181 atgcaatatt tctgctttaa aagttaaaag caaagcgcat tattcaataa acatagcaca
     3241 aaataacggg ggcggtggtc ggcgagcata aatgtcggca ttcctcacga aatgccggac
     3301 aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac tgacggagcg
     3361 tacccgccgt ggcatgaacg ctaccgccta agtaacgcac atcgtcgatg acccaacact
     3421 ggccttcctg aatcattaac acttcatcct gccaaccctg gtcaccctgt ttgagatcca
     3481 cgcgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg gcaacgtggg
     3541 cgctatctgg caaggtggtt cgactggaga atggatcgtt ggtcagtagt tcccgatggt
     3601 tattatcccg ggaggcatcg ctaagcagtg tcgccagttt gtcgctcaga taagggcgca
     3661 aggcggtgat gtcgttgctg cggtgcaaaa tgcggtagtc ataaaattgc tgggccacgt
     3721 tatccgggcc tccttcaacg caaggaccac tgcgtgtgcc gttatcttta taagctggag
     3781 tgactgtggt gcaggcactg aggagcagtg cgcaggggat aagcattgtc aatttgctgt
     3841 agcgcataat gatttcctta taagcgatcg ctctgaaagc gttctacgat aataatgata
     3901 tcctttcaat aatagcgtat cagtctgata atgcttttga gatcgaaggc ttagcaaaca
     3961 aggagatcga tcatgcaatt ttctacaacc ccaactctgg aaggccagac catcgttgaa
     4021 tattgcggtg tggtgaccgg cgaagcgatt ttaggtgcca atattttccg tgatttcttt
     4081 gccggtatcc gcgatatcgt tggcggacgt tccggtgcgt atgaaaaaga actgcgtaaa
     4141 gcacgggaga tcgcctttga ggaattaggc tcccaggcgc gggcgctggg ggccgatgcc
     4201 gtcgtcggta ttgatatcga ctacgaaacg gtcgggcaaa acggcagtat gctgatggtt
     4261 agcgtcagcg gtacggcggt gaaaacgcgt cgatgagaag attcttctgg ctggtcgctg
     4321 ccgctctgtt attggcaggg tgtgcaggcg aaaaaggcat tgtcgagaaa gagggatatc
     4381 agcttgatac ccgacgccag gcgcaggcgg cgtatccgcg cattaaagtg ctggtgatcc
     4441 actacaccgc agatgatttt gatagctcgc tggcgacact gaccgataag caggtcagct
     4501 cgcattatct ggtccctgcg gtaccaccgc gatacaacgg taaaccgcgc atctggcaac
     4561 tggtgccgga acaagaactg gcctggcatg cggggattag cgcctggcgc ggggcaacgc
     4621 gccttaacga cacctctatt ggcattgagc tggaaaaccg tggctggcaa aaatcggccg
     4681 gagtgaaata ttttgccccg tttgaaccgg cacagattca ggcgcttatt ccactggcga
     4741 aagatattat tgcccgttat cacatcaagc cggaaaacgt agtggcacat gcggatatcg
     4801 caccgcagcg caaagacgat ccggggccat tatttccctg gcagcaactg gcgcagcagg
     4861 ggattggtgc ctggccggat gcgcagcggg ttaactttta ccttgccggg cgcgcgccgc
     4921 acacaccggt agataccgcg tcattgctgg agcttttggc gcgctacggt tatgacgtta
     4981 aacctgatat gacaccgcgc gagcagcggc gcgtgattat ggcattccag atgcatttcc
     5041 gcccgacgtt atataacggc gaagcggatg cagaaactca ggcgattgcc gaagcattgc
     5101 tggagaaata cgggcaggat tagcgcggca gttttccgtg gtcgcgtagc caggcggcag
     5161 ttttctcgat accttcatcc agggtgatga ccggctgata acctaactct tcctgcgcac
     5221 gcgtaatatc cagcgtaaag tcaaaattca acttggagac gccgtagtgg gtcagcggcg
     5281 gctcttttgc tgacttgcgg cctaaacgct ccatgctgcg ggcgatcata tccagcatcg
     5341 ggtaggggac ggaacgaata cgacagtcaa tattcaactc gtcgatcagc ttctgcacga
     5401 tgctgcgcag tgtgcgatgc tcgccgttgg tgatgttgta cacacgcccg gaaggtagct
     5461 tatcgcaggc ttcctggctt gccagccaca ttgcgtgcac ggcattttca tagtaggtca
     5521 tatcgaccag cgcactgccg ccatgcggta acagaatact gccgtagtgg tgcatcatat
     5581 gcgccagacg gggaataaag actttatcgt gcggtccgaa cagactttgt gggcgcagaa
     5641 tagtaaagcg cgtttgtgga ttcgcctgcg aaagcatatt gatcacttct tcgctggctg
     5701 ctttgctgcg ggcaaactcg ttggcgaagc ggtgagggcg aaaatcttct ttaatatcgc
     5761 gatggtggtg ataatcgaag tacagggagg gggaagagat atgaataaag ttacgcacac
     5821 cccaggcgac agcccattca cccaggcggc gagtggcgcg aacgttagcc agatcgaaag
     5881 cctgttgtgt cccccagggt gaggtaaagc tggagcagtg ccacagcgta tcaatgcccg
     5941 cgagcatcac tttagcttgt gatgaaacca gctcggtcag atccgccgga acaaactctg
     6001 cgcccatttt ttccagcaat ttgcccattg cctcgttgcg accggtcgct cgcacgctga
     6061 tgcctttctg gcataaaaac tctaccgcgt ttcgacctaa gccgctggtg gcgccggtaa
     6121 ccagtacctt catatcaatc cactgttgtt gagaaaataa cgtgcgcatt cttccgtgat
     6181 ttcccccatg atgcaatggg aaacatgaaa gaataacgca ggttttgtcg attaatctgt
     6241 gctttgttct gccagtctgg cgatttgttt tgccattccg cgaaaaataa acagatgcgc
     6301 ggggatcatc aataaccagt aaaacagccc cggcataccg tgcggatgcc agaaagcgcg
     6361 gacatcgata gtacgatagt cgcctttatc ttccaggcta aaacacagtc gtcccagccc
     6421 cggcgctttc atgccaaata acaacgtaag ttgtttttcc ggttcaacga caatcacttt
     6481 ccagctatcc accgcatcgc cagtctgtaa atattcgcgc tccgggcggc ctttcgccag
     6541 cttatgaccg atcgcgcggt ccatcaacgc ccgtgtctgc cacaaaatat tgccaaagaa
     6601 ataacgctct ttaccgccga tttggttcac tacctgccat aaagcagcaa ggctggcgga
     6661 cgttttaacg gtaaaccccg cctgtttggc aaaataaccg tactccggtc gccagcgggc
     6721 aaaggcctga gcgtcgtagc cccagtcgct ggagttgacc agtttttcct cctctttcaa
     6781 cgtgctacgt accgcgtcat cgaaagcgat cagccgttgt gggatgagtg cacgtagcgc
     6841 ggtatcatcc gccagcagat cgtgtttcag cccctgaatc aacgccc
//
LOCUS       DBBGLQ010000169         6834 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.169, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000169 DBBGLQ010000000
VERSION     DBBGLQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.169"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..194
                     /locus_tag="ACIYSB_004939"
     CDS             <1..194
                     /locus_tag="ACIYSB_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HGC5431800.1"
                     /translation="WGADRNQDPFIWGAILGEEVGEFHQAVLHDRFGGKAAGTSREEA
                     VQIAAVALQIIEYYDRVID"
     gene            complement(497..1011)
                     /locus_tag="ACIYSB_004940"
                     /pseudo
     CDS             complement(497..1011)
                     /locus_tag="ACIYSB_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1619..5032
                     /locus_tag="ACIYSB_004941"
     CDS             1619..5032
                     /locus_tag="ACIYSB_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGC5431801.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIRAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEGLKNTDQVTQEHPVKEQPTATGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPIIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNSVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWHQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAMRFDMDILAAQTARYTNPEELDVLWPVIVEKVERLPPHLAQVQQQGPRINQLRD
                     LGWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTENRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQSELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIEQIARLQKIVEELGVVTDAATV"
     gene            5096..6730
                     /locus_tag="ACIYSB_004942"
     CDS             5096..6730
                     /locus_tag="ACIYSB_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGC5431802.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVEAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLEAVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGEKLASLLKQQYPEGIDAGVDFKDRTQAAFIVPKEDIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
BASE COUNT         1854 a         1661 c         1732 g         1587 t
ORIGIN      
        1 aatggggggc agaccgtaat caagatcctt ttatctgggg agcgattctc ggagaagagg
       61 tgggagaatt tcatcaggct gtcctccatg atcgttttgg tggtaaagca gccggtacca
      121 gtagggagga agcagtacaa attgctgctg ttgcactgca aatcatcgaa tattatgatc
      181 gcgtaataga ttgataaatt agaattaatc aactaagagt cttttacacg cgaagatgaa
      241 ctctggcagc catctacctt cgtgggcctg tagaacacat ctaattgcaa tactcccagt
      301 taatcactaa ccagcctctc cagtaaggct ggtcgattag gtttaattaa acagatataa
      361 caagcaattc acttggtatt acgcggttca cacagacttc tttagttgtg tcatatccta
      421 taactttcag aggcttattt acggtattat cacgaacggt gcaaaagtgg ctcttccgca
      481 acctaggtgg agattttcat gccagtggac tcattaccct ctgcctaagt agttcaaatc
      541 cggctcgacc atacatcaga cgtttcagca tcttcaggcg atttacatga ccttcgacaa
      601 caccgttact ccagcggctg ctggttgcct cacatatagc cgcagcatca gcttccatcc
      661 ccgcagccac gcgccgaagt tctgctgaac cgttttcgtg gactcgagtg aaccaactgc
      721 ttagatttga tttatttggg gttttcagaa tacggtagaa ctcaagtact agttgctgcg
      781 ctattttcag cgccggttct ttttcacaca tcagactaat aaatcgggaa gcataatttt
      841 cttcacccct gattattatc cagggcatca accagcggct tacccgggac actgaaggaa
      901 gtctggcggc agtagtaact ggtgggttcc agcctttacg ccatttggca acagcatccc
      961 tgacgatggt ttcactgcct gtaacccccg ggccaccatt tcccgccata tccagtcgct
     1021 ggtgtgctga attggtcatg tatgaaggaa gatatcaatg gtggtatctc cctttcaaaa
     1081 ggaagtgaaa agcttgatgc ctgctgccag gcaatcaata ttacgtgctt ttaacgacta
     1141 tcctaaatgg tggtagctat gcagagctcc cggtcctagc gctgggagct tatcgcttat
     1201 ctttaccgta agcgtaaacg gaccgccgta tgtagccatt aaaccagtat tggtaacgta
     1261 gatacccacc ttttcaaaag ggaagtggat aaaagatagc gaaaatgcga agagttgatt
     1321 aatgcattga aacaaaattc attaatgatg aacttggaga aaagctccat cgttttattg
     1381 gctcaaaaaa catatcccat agatgatcag ccataccttc attggttagc aaggtatcct
     1441 gtgagtagcc atggctgaag atgcccattg atgggcgaaa accaaggatc tgaccgttag
     1501 agcagttatg tcgttaaaca catcaccacc atatgccatc cggctcactt cgtcggccaa
     1561 attagtgtga tattctttcc agaaatgctg ttgtatccac gacataaggc gatgattgat
     1621 ggagcagtct ctcaattttg aaatgttgcg cagccagtgg ccggaactgg ctgaactcgc
     1681 gtgtatggcg gaacgctatg ttcactccga tccggaaagt tgtctggtca agctgcgcaa
     1741 ctacaccgaa ttgatggtgc gctggttgta tcgtcaggag cggttgccgg aaggtattaa
     1801 ggctaatctt tacgatttaa tgaatgctga tgtctttacc agcatgatgc cggaagccat
     1861 catcatgaaa atggatgcgc tgcgtatcca tggcaaccgt gccgcgcacg gcggacgtat
     1921 caaagctaaa gatacttact ggctgctcaa agaagcgtat ttgttgggaa tttggctgta
     1981 tgttcgctac gcccacggta atgttgatga ctgccccaaa tttacactcc ctccattaac
     2041 acaatcttca ggtcgtgcag atgaaaaacg tctggaagat gcaatcaggg ctcaggatga
     2101 aagccgtgag cgcgaactgg cgctgcaacg cgcactacag caagaacagg aaaaggccga
     2161 acacctcact caacgcctga atgaagccag agcgcgtaac cagcatgttg ccgatattct
     2221 ctctattgat gaagcggaaa cccgccgtcg cctgattgac tctcgcctgc ttgctgctga
     2281 ctggaatgta ggcgaaggcc ttaaaaatac cgatcaggtt acgcaggaac atcccgttaa
     2341 agaacaacct accgcgaccg gagacggtta tgcggactat gtcttgtggg atgaggcaca
     2401 caaaccgctg gcggtggtgg aagcaaaaaa aaccagcgtc aatgccgagc aaggacgaat
     2461 tcaggcccgg ctgtatgcag actggctgga aaaagaatac ggccagcgtc cgatcatctt
     2521 ctacaccaac ggctatgata tctggctgtg ggatgaccat aaaactcatg gttatccccc
     2581 gcgtcgggtg tttggcttct acagcaagga aagcctgcaa tatcttattc aacagcgtga
     2641 aacccgtctt ccgctgaaca gtgtgccgca cgtaaaagat aacgaaggta aggccgttgc
     2701 cggacgtttg taccagcttg aaaccattgc ccgtgtcagc gagcggttta ccaacaaata
     2761 ccggcaatcg ctaattgttc aggctacggg tacaggcaaa acccgcgtgg caatagcgct
     2821 aagcaaactg atgattgatg cccgctgggt aaaacgcgtc ctgtttcttt gcgaccgcaa
     2881 ggaactacgt aaacaggcgg cgaatgcctt taatcaattc accaatgagc cgctgtatgt
     2941 ggtcgggaaa tcgaaaaaag cagacaggca gaatgccaga atctacatcg ccacctatcc
     3001 tggtatgatg aaaatcatgg accatttcga tgttggttat ttcgatttga tcatcgccga
     3061 tgaatcccat cgctctatct acaacgttta cggcgatctg tttaagtatt ttgatgccct
     3121 ccagattggc ctgacggcca cgccgattga catggtgagt aaaaccactt tcggcctgtt
     3181 tggttgtgaa ggacgtattc ctaccgccaa ctacagcctg gaagatgcca tcgccgataa
     3241 caatctggtt ccctatgagg ttgtcacgca caccacagaa ttcctgcgcg aaggcatcaa
     3301 acgggagaaa ttaagcgacg cacaaatccg cgagctggaa gaacaaggca tagatcccaa
     3361 tacgctggag tttgatggca aggcgctgga tgaggcgatc tacaacaaag acaccaaccg
     3421 ctatatcctg cgtaacctga tggaaaatgg cctgaaagat cgggacggcc agttgccagg
     3481 taaaaccatc attttcgccc gtaaccacaa acacgcgctg ctgttgaatg aattattcga
     3541 cgatatgtac ccgcagtttg ccgggcgctt ctgccaggtc atcgacaatt acgatccccg
     3601 cgccgagcaa ctgattgatg attttaaagg gctggatgaa agcaccaaca aagagctgac
     3661 catcgctatc tccgttgaca tgctcgatac cggtattgat gtcccggaaa ttgtgaatct
     3721 ggtctttgcc aaaccggtca aatcaaaagt gaagttctgg cagatgattg gccgtggtac
     3781 gcgcctctgt ccggggttgt acggttacga cgataacggc aaacctctgg ataaacagaa
     3841 attccgcatt ttcgatcact ggggcaattt tgagtatcac gaactgcata ccgaagaggc
     3901 cgaagtcaca gcgacaaaat cgcttgcaca aaaacgcttc gaagcgtggg ttatgctggg
     3961 ggccgctgcg caacgtaaat tcgacaaaca ggcggtggat ttagttgctc accagctacg
     4021 cgagcaaatc aatgcactgg atgaaaagtc gattgccgtc caggaaaagt ggcatcaaaa
     4081 agcgcagtac agcgatgaaa aagtgctgcg ccagctttcc ccgaaaacac agcaggattt
     4141 gctatctgtc ctggccccgc tgatgcagtg gctggacgtg cgcgggcaaa gcgacgctat
     4201 gcgctttgat atggatattc tggcggcgca aactgcccgt tataccaacc cggaagagct
     4261 ggatgtgctc tggccggtca tcgtcgaaaa agtggagcgc ctgccgccgc atttggcgca
     4321 ggtccaacag caaggaccgc ggatcaatca gcttcgtgat ttaggctggt ggaagcaggc
     4381 cagcctggaa gagctggaag atatccgcat tcatctgcgc ggcatcatgc acctgatgga
     4441 aaaagacgcg acgccgaaat ttggttcgat acaggtggat attaccgaag atgcgaacct
     4501 gattcagacg gaaaaccgca aaaccaacat ccgctcgatt gacttcaaac tctatcgcca
     4561 gcaggtgcag ggagcgctgg agccgctgtt ccagcaaaat ccggtgctga agaaaatccg
     4621 caacggcgag cctgtgacgc aaagcgagct ggatgaactg gctaaactgg tgctgatcca
     4681 gaaccctaac gttgatatcc gggcactgaa agagttctac ccgcaggcga ccgccagcct
     4741 ggataaacta ttgcgtacca tcatcgggat ggacagcgac gcggtggaag tgcgctttgc
     4801 tcagttcgcc gctgataaca gcctgaccag ccagcaattg cgcttcctgt cattgctgaa
     4861 aaaccacatt cgcgattacg gcaccattga aatgcggcag ctctttgaac agccttttac
     4921 gcatatccac aacgaaggcg ttaccggtgt gttccctgat atagagcaga tcgcccgcct
     4981 acaaaagata gtcgaagagc tgggtgttgt gaccgacgca gcaacggtat aatacgcaca
     5041 tgacaagccc cggcagcggg gcttttgtat ttaaaacagg atataaaagc taacaatgat
     5101 cactggtgac ttaaaaagta aaatcgacgg cctgtgggaa gatttctggg tgggtggtat
     5161 caccaacccg ctgaccgtaa tcgaacagat cacctatcta atgtattccc ggatgctgga
     5221 tactcaggaa caacgcgacg agaagcgtaa acaaatcgcg ggtattgatt ttaaaccgcg
     5281 ttttgcacca gaacagcagg agttccgttt cagtcactat agcaatcttg gctcggatga
     5341 gatgatggaa gtggtgcgcg atggtgtatt ccagcatttt cgtcagctcg gccaggctga
     5401 tgcgtcgaag gtaacgctgc tgggcaactt tatgaaagat gcccgtctgg agattgttaa
     5461 gccttcattg ctgaccaaag cggttgaggt gatcaaaaac ctgccattgg atcgcggcga
     5521 caccaaaggc gacctttacg aatacctgtt aagcaagctg accactgccg gaatcaacgg
     5581 ccagtttcgc acaccgcgcc acattatccg cacgatggtt gaaatgatgg agccgaaccc
     5641 cgcccgcggc gagacgattt gcgatccggc ctgcggcacc ggtggctttc tggcaaccag
     5701 ctatgaatat ctactggaga agtacagctc gctggaatcc attcataccg agattggcac
     5761 caacgaacgt ggtgagctgg aagagcaaaa aatctttacc ggcgatctgt tgaccccgtg
     5821 gcgtaaccat gtggataaca atatgttcca cggttacgac tttgacacca caatgttacg
     5881 tatcgccgcc atgaacctga ttatgcacgg cgtggaagcg ccggatatcc actatcagga
     5941 caccatgagc cagagcttca gcacaaactt cccgcaggcc agtaaaaacg ctttcaacct
     6001 gattctggca aacccgccgt ttaccggttc actggacgaa gaagatatcg actccacgct
     6061 gtcggcaatg gtgaaaacca aaaaaaccga actactgttc ctggcgcgta ttctgcaaat
     6121 gctgaaagtg ggcgggcgta gtgccactat cgtgccgcag ggcgtgctgt ttggctccag
     6181 taaggcgcac cagtcacttc gcaaaacgct ggtggaagat aaccaactgg aagcggtgat
     6241 caacctgcct tccggcgtat ttaaacctta cgctggcgtg gcgacggcga tcctgatctt
     6301 taccaaaggt ggtcagacgg acgaggtctg gttctacgat ctgcaaaatg atggctacag
     6361 cctggatgac aagcgtaacc caattaaaga caacgacctg ccgcatctgc tggcaagctg
     6421 gaagcattac cgtactttac gcgggctgcc ggttgataac tttatgggtg agaagttagc
     6481 ctcgttgctg aaacagcagt acccggaagg gattgatgct ggcgttgatt ttaaagatcg
     6541 cactcaggcg gcgtttatag tgccaaaaga agatattgct gcccagaaat acgatctctc
     6601 tattaatcgt tataaagaag tggtgtatca ggctgaggag tatgaagatc cgaaggtgat
     6661 attgaagcgg ttaaaagatc tggaaaaaga gattctggcg gatctggatg agctggaggg
     6721 gatgctgtga aaaaaaatca attggtcaca cttggcaaac atatagacat attgtccggg
     6781 tgtgcattcc cttcaagtgg attcaatagg aacaatggtg ttccactaat ccgt
//
LOCUS       DBBGLQ010000170         6822 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.170, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000170 DBBGLQ010000000
VERSION     DBBGLQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.170"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..519
                     /gene="istB"
                     /locus_tag="ACIYSB_004943"
     CDS             <1..519
                     /gene="istB"
                     /locus_tag="ACIYSB_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HGC5431803.1"
                     /translation="KQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGYEAVRAGI
                     KVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDETGYLPFSQEEAKLFFQV
                     IAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYRLRQK
                     RKAGVIAEANPE"
     gene            complement(591..1126)
                     /locus_tag="ACIYSB_004944"
                     /pseudo
     CDS             complement(591..1126)
                     /locus_tag="ACIYSB_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            complement(1146..1430)
                     /gene="tnpB"
                     /locus_tag="ACIYSB_004945"
                     /pseudo
     CDS             complement(1146..1430)
                     /gene="tnpB"
                     /locus_tag="ACIYSB_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1532..2704
                     /gene="istA"
                     /locus_tag="ACIYSB_004946"
     CDS             1532..2704
                     /gene="istA"
                     /locus_tag="ACIYSB_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HGC5431804.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIVTQIGCSERTVRRYLKYPEPP
                     ARRTRHKMVKLKPFMDYIDMRLAENVWNSAVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNALGFSRRFHVFAAPKQDA
                     ENTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEYLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHTPLWQQVSQVEHRLLSAYEELL"
     gene            2704..3231
                     /gene="istB"
                     /locus_tag="ACIYSB_004947"
                     /pseudo
     CDS             2704..3231
                     /gene="istB"
                     /locus_tag="ACIYSB_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
     gene            3369..4445
                     /locus_tag="ACIYSB_004948"
                     /pseudo
     CDS             3369..4445
                     /locus_tag="ACIYSB_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4462..4605
                     /locus_tag="ACIYSB_004949"
                     /pseudo
     CDS             4462..4605
                     /locus_tag="ACIYSB_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5311..6570
                     /gene="amiC"
                     /locus_tag="ACIYSB_004950"
     CDS             5311..6570
                     /gene="amiC"
                     /locus_tag="ACIYSB_004950"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461908.1"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGC5431805.1"
                     /translation="MLVSNYNYVISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVW
                     PASSYTRVTVESNRQLQYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKS
                     ARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALL
                     EDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAVGKYKTREKDVVL
                     QIARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQP
                     SGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADS
                     LKLGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTAR
                     FQQQVAESILEGIKEYFSDIEALARKA"
BASE COUNT         1806 a         1584 c         1727 g         1705 t
ORIGIN      
        1 aagcaactcc agtcgttacg ctcactcagc ttcatagaac gtaatgaaaa tatcgtatta
       61 ctggggccat caggtgtggg gaaaacccat ctggcaatag cgatgggcta tgaagccgtc
      121 cgtgcaggta tcaaagttcg cttcacaaca gcagcagatc tgttacttca gttatctacg
      181 gcacaacgtc agggccgtta taaaacgacg cttcagcgtg gagtaatggc cccccgcctg
      241 ctcatcattg atgaaacagg ctatctgccg ttcagtcagg aagaagcaaa actgttcttc
      301 caggtcattg ctaaacgtta cgaaaagagc gcaatgatcc tgacatccaa tctgccgttc
      361 gggcagtggg atcaaacgtt cgccggtgat gcagcactga cctcagcgat gctggaccgt
      421 atcttacacc actcacatgt cgttcaaatc aaaggagaaa gctatcgact cagacagaaa
      481 cgaaaagccg gggttatagc agaagctaat cctgagtaaa acggtggatc aatattgggc
      541 cgttggtgga gatataagtg gatcactttt catccgtcgt tgacatgagc cggcgcctgc
      601 acgatatggt cacaccgaca acaggccagt ttcggacttg tgtttcgata accttaaagg
      661 cgcagctgat aagctccagc tgctctgaca catcacatcc cagagaactg agttcaccac
      721 cacaggcagg acagcattcc tcttctggcc ggataacccg ggtttcacgg ggaagtgagg
      781 ccggtaactg tttacaggct gaagactgac gcagggcggc ggatggcagt accgggtcat
      841 attgctcacc cagcgtttcc gccatttctt cctgaagtgt gctgattctc tcctgtgctt
      901 cctgtatctg tcgttcggtt tttgcgcgaa gtttttctga gcttttaccg aaatgcatac
      961 gttgcagttt cgcaaccagc gccttcagcc ggttgatttc agaagcataa gccgccaccc
     1021 tctgtgagag caggcggttg tattcagcca tctggcggat ggtgtcctgt tgcgtctgca
     1081 acagtgcccg caggcgggga ttctcatgag caagtgaggt gtccatatac tcgctttgca
     1141 acgggttata tccggatgcc aggccgttct gtccgtttgg gatgttgcca cgcgatcccc
     1201 tccagtagca tggataactg agctggcgtc aggtgcactt tcccttcccg ggtcaccggc
     1261 cagatgaagc ggccccgttc caggcgtttg gcgaacaggc ataacccatc acgatcggcc
     1321 cacagtattt tcaccatttt gccactgcgg ccccggaaga cgaagatatg cccggagaac
     1381 gggtcatcct tcaggacgtt ctgaactttt gaggacaggc cgttaaagcc tgtcagcgcc
     1441 agtgatataa gacggtaatt caccatttgg attgtccgct ccacccaaca tgttgtttcc
     1501 ttaaggttct cacaccagaa aggacatcaa catgctgagc agagaggact tttacatgat
     1561 aaagcaaatg cgccagcagg gcgcgtacat tgtcgatatt gtgactcaga ttggttgctc
     1621 tgaacgaacg gtcagacgct acctcaaata ccctgaaccg ccagccagaa ggacccgcca
     1681 caaaatggtt aagctgaaac cgtttatgga ttacatcgac atgcgcctgg cagagaatgt
     1741 ctggaatagc gcggttatct tcgcggagat taaggcaatg ggctatacgg gcggacgttc
     1801 catgctgcgt tactacatcc agcccaaacg taaaatgcgt ccgtcaaaaa gaacagttcg
     1861 cttcgaaact cagcctggat accagctcca gcacgactgg ggcgaagttg aggtggaagt
     1921 tgccgggcaa cggtgcaaag ttaactttgc ggttaatgcg ctggggttct cccgccgctt
     1981 tcatgtcttc gctgccccaa aacaggatgc tgagaatacc tacgaatcac tggttcgcgc
     2041 cttccgctac ttcggtggtt gtgtgaaaac ggtgctggtt gataaccaga aggctgcggt
     2101 gctgaagaat aacaacggga aagtcgtgtt caactccgga tttctgttgc tggctgacca
     2161 ctataacttc ctgccacggg catgccgtcc acgcagggcc agaacaaaag gtaaggttga
     2221 gcggatggtg aaatacctca aggagaactt cttcgtccgg taccgcaggt tcgacagctt
     2281 cactcatgtc aatcaacaac tggagcaatg gatagccgat gtggctgaca aacgggagct
     2341 tcgtcagttc aaagaaacgc cggaacagcg cttcgcgctg gagcaggaat atctgcagcc
     2401 gttaccggat acggacttcg ataccagtta ctttgacatc cgccatgtgt cctgggacag
     2461 ctatatcgag gttggtggta atcgttacag cgttcccgaa gcgctgtgtg gtcagccggt
     2521 atcgatacga atatcgctgg atgacgagtt gcggatctac agtaatgaga aactggtggc
     2581 ctcacatcgc ctctgttcgg catcgtctgg ctggcagaca gtgccggagc atcacacccc
     2641 gctctggcag caggtcagtc aggtggaaca tcgactactg agtgcctatg aggagctgtt
     2701 gtgatgcatg agctggaagt cctgctgagt cgcctgaaaa tggagcatct gagttatcac
     2761 gttgaaagcc tgctggaaca ggcagctaaa aaagagctga actaccggga gttcctgtgc
     2821 atggcgctac agcaggaatg gaacggcagg catcagcgcg gtatggagtc caggctgaag
     2881 caggctcgtc tgccgtgggt caaaacgctg gagcagttcg actttacctt ccagccgggc
     2941 atctaccgta aggttgtccg ggaactggct ggtctggcgt tcgtggagcg cagcgaaaac
     3001 gtgatcctgc tgggacctcc tggtgtcgga aaaactcatc tggccatagc tcttggcgtg
     3061 aaagcggtgg atgcgggaca tcgggtactg tttatgccac tggacagact gatcgcgaca
     3121 ctgatgaaag cgaaacagga aaaccggctg gagcgtcagc tacagcaact gagttatgcc
     3181 cgggtgttga tcctggatga aataggctat ctgccgatga acagagagga tgtcggaaac
     3241 gccttcatgc gaggcgtctc ctcaccgatg cgcgtgactc aagaagggcc tgacggcttg
     3301 tctcgttact gtcctgtccg ggttatctgt ctggagattc aactctgttt cctcacagga
     3361 gctctgttat ggcaggtaaa gttacggaaa ccgctgttgt gggtggcgtg gatatacata
     3421 aagatctgca cgttgccgct gtcgtagatc agaacaataa agttctgggg accccgtttt
     3481 tctccacaac acggcaaggt taccggcaga tgctggcatg gatggcttcg tttggggcat
     3541 taaagcgaat tggtgttgag tgtacaggca cctatggatc aggtctgctt cgctatttac
     3601 agaatgccgg gttagaagtt cttgaggtgg ctgtgccaga tcggatggag cgacgcaaac
     3661 ggggtaaaag tgacacgatt gatgctgaat gtgccgctca cgccgcatgc tccggaataa
     3721 gaactgtcac acccaaaacg cgcaatggta tgattgagtc tctgcgggta ttaaaaactt
     3781 gccgaaaaac agcaatatca gcccgctgag tcgctctcca gattaaccat tccaatatta
     3841 tctctgcccc ggatgaatta cgtgaacagc tcagaaatat gacgcgcatg cagctcatca
     3901 ggactctgag atcctggcgg cctgatgcca gtgaatagct caatgttacc aacgtttatc
     3961 gtatttcatt aaagtccctt gcccgacgct atctcgagtt acatgacgaa atcgctgatt
     4021 tggatgttat gattgcggca attgtggatg agctggcacc tgcactgatt aaacgtaatg
     4081 ctattggata cgaaagcgct tcacagttgc tgatcactgc cggagacaat ccccaacgat
     4141 taagatcaga atcaggtttt gcggcactgt gtggtgtcag ccctgttccc gtatcttcag
     4201 gaaaaacgaa tcgttatcga cttaaccggg gtggagatcg tgctgcaaat agtgcacttc
     4261 acatcatttc catcggacgt ttgcgaactg acgataaaac gaaggaatat gtcgccagac
     4321 gagtagcgga agggcataca aaaatggaaa caatacgcag cctgaagcgc tatatctcac
     4381 acgaagttta tacattactg cgtaatcaaa acaggcagat caacagcacc ccgataactg
     4441 cttgactctt agaagggcgt ccagggcagt cattatacgc gtaggcagtt ccggcagtta
     4501 ttgtggcgat accagatcag gcagagtatg agccggcgcg gaaactgctg ggataacagc
     4561 ccaatggaac gcttcttcag gagtctgaag aacgaatgga cgccccgctc ttcaactttg
     4621 gtaataccaa aatcagacga cggtgttata ggtgcttgaa ctgaatttac tttgaaaaaa
     4681 tgtaaaagca tttaaatcat agccagaagc cgcgattatt cagtgcttct cattttatat
     4741 tttctgcaaa tttatatgtg gtataaaata acacccttgt cattcaggta taaaatatca
     4801 tcatcgtaca gtaaaggata tataaatata aaattatact attcaaaata tggtgcattc
     4861 ttattcatga taggaatact tagaacatca ttttttcatt gtagaccatg atttcggatt
     4921 tgaaaagttc cttccccagt aatctctagt ttttattctc aattcaaccc aactcttccc
     4981 tatacaaact aaaaaacatt gttctattga tagatacttc tcagtaatca gataaaaaac
     5041 tataatattg ctgtgcatga tgtacatatt taaaatgtca tgagtacatc ccttttattt
     5101 gaaaagcaaa ctaaaagaaa tcacttaaat aaaatgtttt tatatcgtgg tatttaagaa
     5161 aacttatatc aaaaaaataa gatattgacc tttatttaaa attagattag attgcaaaga
     5221 taagaatacg tgtgattatt tctttaaaca cctgtaaaaa tttattagag catagttata
     5281 ttattgaaca ttgtattgct taggtaaggt atgctggtat ctaactataa ttatgtaatc
     5341 agccgtcgtc gtttactgca aggtgcgggc gccatgtggc tattgagcgt aagtcaggtc
     5401 agcctggctg cggtcagcca ggtcgtggcg gtacgcgtct ggcctgcgtc cagctacacc
     5461 cgcgtgacgg tagaatcaaa tcgtcagctg caatataagc agttcgcgtt aagtaaccct
     5521 gaacgtgtgg tggtggatat cgaagatgta aacctgaact cggtactcaa ggggatggcg
     5581 gcacaaattc gtgcagacga cccgttcatc aagtcggcgc gcgtcgggca atttgacccg
     5641 caaaccgtac gcatggtttt tgaattaaag caaaacgtaa aaccgcagct gtttgccctt
     5701 gcgccggtcg ccgggtttaa agagcgtctg gtgatggacc tctatccggc caatgcacag
     5761 gatatgcagg acccgctgct ggcgctgctg gaggattaca acaaaggcga cctcgaaaag
     5821 caggtgccgc cagcacaaag tggtccacaa ccgggtaaag cagggcgtga tcgtccgatt
     5881 gtcattatgc ttgaccccgg ccacggtggc gaagaccccg gtgcggtggg gaaatacaaa
     5941 acacgcgaaa aagacgtggt attgcaaata gctcgccgtc tgcgctctct gatcgaaaaa
     6001 gagggtaata tgaaggtgta catgacgcgc aatgaagaca tcttcattcc gttgcaagtg
     6061 cgtgtagcaa aagcacagaa acaacgtgct gacctttttg tttctatcca tgctgatgcc
     6121 tttaccagtc gccagccgag cggttcttcc gtgtttgcgc tttcgaccaa aggcgcgaca
     6181 agtactgcgg caaaatatct ggcacaaacc cagaacgcct cggacttgat tggcggcgtg
     6241 agcaaaagcg gtgaccgcta tgtcgaccac accatgttcg atatggtgca gtctctgacc
     6301 attgccgaca gcctgaagtt gggtaaagcg gtgctgaata agctcggtaa aatcaacaag
     6361 ctgcataaaa atcaggttga acaggccggg tttgccgtgc tgaaagcacc tgatattccc
     6421 tccattctgg tcgaaacggc gtttatcagt aacgttgagg aagagcgcaa actgaaaacg
     6481 gcaagattcc aacaacaagt ggcagaatct atcctagagg gaattaaaga gtatttttcg
     6541 gatatagaag cgttagcgag aaaagcataa tttaaaaatg tttaatattc tatgttctcg
     6601 gtcatatttt attaatttgg atgaatatat gctgttagtg ctgtattctc cgtttggata
     6661 cacctttacc ttagcgatat agttgtgtta ttcatatata aatatgcccc gttttaattc
     6721 catgcatttt tatatcttgg tcaaataatg gtgtcgtcga tattcttcca gcgtcttatt
     6781 gttcagtgat tcatgtgtgc gttcatagct atattctgat aa
//
LOCUS       DBBGLQ010000171         6812 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.171, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000171 DBBGLQ010000000
VERSION     DBBGLQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.171"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            8..292
                     /gene="traQ"
                     /locus_tag="ACIYSB_004951"
     CDS             8..292
                     /gene="traQ"
                     /locus_tag="ACIYSB_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HGC5431806.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
     gene            279..824
                     /gene="trbB"
                     /locus_tag="ACIYSB_004952"
     CDS             279..824
                     /gene="trbB"
                     /locus_tag="ACIYSB_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HGC5431807.1"
                     /translation="MSLTNLLPVILLMMATGVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            754..1116
                     /gene="trbJ"
                     /locus_tag="ACIYSB_004953"
     CDS             754..1116
                     /gene="trbJ"
                     /locus_tag="ACIYSB_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HGC5431808.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGRKYRD
                     IPAEMSYAPGEQIKGGQE"
     gene            1116..2489
                     /gene="traH"
                     /locus_tag="ACIYSB_004954"
     CDS             1116..2489
                     /gene="traH"
                     /locus_tag="ACIYSB_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HGC5431809.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRVMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            2486..5307
                     /gene="traG"
                     /locus_tag="ACIYSB_004955"
                     /pseudo
     CDS             2486..5307
                     /gene="traG"
                     /locus_tag="ACIYSB_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
     gene            5322..5825
                     /locus_tag="ACIYSB_004956"
     CDS             5322..5825
                     /locus_tag="ACIYSB_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HGC5431810.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            5857..6588
                     /gene="traT"
                     /locus_tag="ACIYSB_004957"
     CDS             5857..6588
                     /gene="traT"
                     /locus_tag="ACIYSB_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HGC5431811.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
BASE COUNT         1729 a         1562 c         1885 g         1636 t
ORIGIN      
        1 gtttaacatg ataagtaaac gcagattctc tttaccccgg cttgatatta cgggaatgtg
       61 ggtattttcc ctgggtgtct ggtttcatat cgtcgcccgt cttgtttaca gcaaaccctg
      121 gatggctttt tttctggcgg agttgattgc ggccatcctt gtgctgttcg gcgcatacca
      181 ggtgctggat gcatggattg cccgtgtcag ccgggaagag cgtgaggcac tggaagccag
      241 gcaacaggcc atgatggcag agcagcagga gggcggacat gtctctcact aacttactgc
      301 cggtcatttt gttgatgatg gccacaggtg tgcaggcctc cacccgggat gaaatagagc
      361 gactctggaa tccgcagggt atggccgcgc agcctgcaca accggcagca gacacgtcag
      421 ccaggacaga aaagccggcc ccccgctggt tccgcctcag caacggcagg caggttaacc
      481 tggccgactg gaaggtggtg ctgtttatgc aggggcattg cccttactgt caccagtttg
      541 acccggtact gaagcagttg gcacagcagt acggcttttc cgtttttccc tacaccctgg
      601 atggtcaggg ggataccgcc tttccggaag cattaccggt gccaccggac gtgatgcaga
      661 ccttcttccc gaatatcccg gtggccacac cgaccacctt cctggtcaac gtcaacacgc
      721 ttgaggcatt accgctttta cagggggcaa cggatgccgc cggttttatg gcgcggatgg
      781 ataccgttct gcagatgtac ggaggaaaaa aaggtgcgaa ataagcaggt ggtgttactc
      841 attgccggaa tatccggcat cgcgacggga ataatcgtca gcctgaatat cccctttatc
      901 catcaggggc ttttttatcc cgccagccct gtagaaattg tcgtttcgct gtgccttacc
      961 ttttctgttt ctgttgtgtt ttttgtgggg gcaattgtgg gatggatttc ggtgtctgaa
     1021 atatattaca gccgcaggac tggacggaaa tatcgggata ttcctgctga aatgtcgtat
     1081 gcgccgggag aacaaattaa gggagggcaa gaatgatgcc acgcattaaa cctcttctgg
     1141 ttctttgtgc tgctttactg acggtcacac ccgcagcgtc agcggatgtg aacagcgaca
     1201 tgaatcagtt ctttaacaag ctgggcttcg cctccaatac cacacagccg ggcgtctggc
     1261 aggggcaggc cgccggttat gcttacggtg gctccctgta tgcccgtact caggtcaaaa
     1321 atgttcagct gatttccatg acgctgccgg atatcaatgc cggatgtggc ggcatcgacg
     1381 cttatctcgg ctcgttcagt tttattaatg gcgagcagct gcagcgtttt gttaagcaga
     1441 ttatgagcaa tgctgccggt tacttttttg accttgccct gcagacaacg gtgccggaaa
     1501 tcaaaaccgc aaaagacttc ctgcagaaaa tggcaagcga cattaacagt atgaacctca
     1561 gttcctgcca ggcggcacag gggattatcg gcgggctttt cccccggacg caggtgtccc
     1621 agcagaaagt ctgtcaggac attgccggtg agagcaatat ttttgctgac tgggcggctt
     1681 cccggcaggg atgtaccgtt ggcgggaaat ctgacagtgt cagggataaa gccagcgaca
     1741 aggataagga gcgggtgacc aaaaacatca acatcatgtg gaatgcgctt tccaaaaaca
     1801 gaatgtttga cggcaacaaa gagctgaaag agtttgtgat gacgttgacc ggctcactgg
     1861 tgtttggtcc taacggcgaa atcacaccct tgtcagccag aaccactgac cgctcaatta
     1921 tccgggtcat gatggaaggc ggcacagcaa aaatttatca ctgcaacgat tctgataaat
     1981 gcctgaaagt ggtggcagac acaccggtga ccatcagccg ggataatgca ctgaagtctc
     2041 agattactaa acttctggcc agcattcaga acaaggcggt cagtgacacg cctctggatg
     2101 acaaggaaaa aggctttatt tccagtacca ccatccccgt cttcaaatac ctggttgacc
     2161 cgcaaatgct cggtgtttcc aacagtatga tttaccagct gacggactat atcggttacg
     2221 acatcctgct gcagtacatt caggagctga tacagcaggc ccgggcgatg gtggccacag
     2281 gaaattatga cgaagcagtt atcgaacata ttaacgacaa catgaatgat gccacccggc
     2341 agattgcggc gtttcagtca caggtgcagg tacagcagga tgcgctgctg gtcgtcgatc
     2401 gtcagatgag ctacatgcgt cagcagcttt ccgcccgcat gctcagtcgt taccagaaca
     2461 actatcactt cggagggagc acgctgtgaa tgaagtttat gtgattgccg gtggtgagtg
     2521 gttgcggaat aacctgaatg ccattgccgc ctttatgggg acccggacgt gggattccat
     2581 tgaaaaaatt gcgctcacat tgtctgttct cgcggtggcc gtaatgtggg tacagcggca
     2641 caacgtgatg gatttgctgg gctgggtggc cgtgtttgtg cttatcagcc tgctggttaa
     2701 tgtccgcaca tcggtgcaga ttattgataa cagtgacctg gtcaaagttc accgggtgga
     2761 taatgtgccg gtcggactgg cgatgccact ttcactgacg acccgtatcg ggcatgcaat
     2821 ggtggccagt tacgagatga tcttcacgca accggacagt gtcacctaca gcaaaacggg
     2881 gatgctgttt ggcgcagaac tggtatcaaa aagcaccgac tttttgtccc gcaatccgga
     2941 aatcgccaat cttttccagg attatgtgca gaactgcgtg atgggggata tttacctgaa
     3001 ccacaaatac acgctggagg agctgatggc ctccgctgat ccctacacgc tgattttttc
     3061 ccgccccagt ccgctacggg gcgtttatga cagcaataat aatttcgtga cctgtaagga
     3121 cgcgtcggtt tcgctgaaag acaagctgaa tctggataca cagagtggcg gaaaaacctg
     3181 gcattattat gccagcagct atttggtgga cgccctgatc cgaacctgtt gttcagtacg
     3241 ctgattggcg acagttacag ttacttctat ggctcaagta aatcagccag ccagatcatc
     3301 cgccagaatg tcaccatcaa tgcgctgaag gaggggatca ccagttatgc cgcgcgtaat
     3361 ggtgattcag ccagcctggt gaatctggcc accacgtcat cgatggagaa gcaacgtctg
     3421 gcacatgtct ccatcggtca tgtggcgatg cggacactgc ccatgacgca gaccatcctg
     3481 acggggatcg ccatcggtat tttcccgctt ctggtactgg cggctgtctt caacaaactg
     3541 acgctgtccg tcctgaaagg ctatgtgttt gccctgatgt ggttgcagtc atggccaatg
     3601 ttgtacgcca ttctgaacag cgccatgaca ttttatgcga agcagaatgg tgcaccggtg
     3661 gtgctctcgg aaatttcaca gatacagctg aaatactccg atctggcttc cactgccggg
     3721 tatctttcca tgatgatccc gccattatcc tggatgatgg tcaggggact cggggccgga
     3781 ttttccagtg tgtacagcca tttcgcttcc tctgctatca gcccgactgc cagtgcggca
     3841 ggtagtgtgg ttgacggtaa ttactcctac ggcaacatgc agacggaaaa cgtgaacgga
     3901 ttcagctgga gcaccaacag caccacgtcg tttggtcaga tgatgtacca gaccggcagt
     3961 ggcgcaaccg ccacacagac ccgtgacggt aatatggtga tggatgcgag cggggcgatg
     4021 tcccgtttac cggtcggtat caatgcaacg cgtcagattg cggcggcaca acaggaaatg
     4081 gcccgggagg cgtcgaacag agcagaaagc gccctgcatg ggttcagcag cagtattgcc
     4141 agtgcctgga actcgctcag tcagtttggt tctaaccggg ggagcagtga ttctgtcacc
     4201 ggtggtgctg acagcacgat gagtgcacag gactccatga tggccagccg tatgcgcagc
     4261 gcagtggaaa gctatgcgaa ggcgcataat atcagtaatg agcaggcgac aagagaactg
     4321 gcatcaagaa gtacgagagg ttctgcgggg atgtatggcg atgctcatgc tgaatggggg
     4381 gttaaaccta aaattcttgg tgttggtggt ggcgtaggtg ttagaggtgg tggtcgggca
     4441 agtattgact ggagtgatga ggatgcgcat caggccagca gtggttcacg ggccagccat
     4501 gatgctcgcc atgatatcga tgccagggct acacaagact ttaaagaggc cagcgattac
     4561 tttaccagtc gcaaggtcag tgaatccggc agccatacgg acaataatgc cgattcccgt
     4621 gtggaccagc tgtctgcagc cctgaactca gcaaaacaga gttacgacca gtacacgacg
     4681 aatatgaccc gcagccatga atatgctgaa atggcatccc gtactgaaag catgagtggg
     4741 cagatgagtg aagacctgtc gcaacagttt gcacagtatg tgatgaaaca cgcgccgcag
     4801 gatgccgaag ctattctgac aaacaccagt tccccggaga ttgcagaacg ccgtcgggct
     4861 atggcgtggt cttttgttca ggaacaggtg cagcctggtg ttgataatgc ctggcgtgaa
     4921 tcccgtgggg acatcggtaa aggaatggag agcgtacctt cggggggtgg cagccaggat
     4981 attattgctg atcatcaggg gcatcaggcc attattgagc aaagaacgca ggacagtaat
     5041 atccgtaatg atgtaaaaca tcaggttgat aatatggtca cagaatacag aggcaatatc
     5101 ggagatactc agagcagcat acgtggcgaa gaaaatattg taggaagaca gtattctgaa
     5161 ctacaaaatc accataaaac agaggcatta tctcagaata ataaatataa tgaagaaaaa
     5221 tcgggtcagg aaagaatgcc cggggctgac agtccacagg aactgatgaa aagagcaaag
     5281 gagtatcagg ataagcacaa gcaataatat tgtatgggga gatgataatg aaaaatttag
     5341 ctcatattgc tctggtgacg gtgatacagt tcattgcctg ttatctggct gattggggga
     5401 gtgccgaaac agtttttatc ctgtgcttta ttgtactatg gcagggattg tttatatggt
     5461 tattttctca aatacgaaaa aaacggcatg tgtcggatga gtttaaattc agtaaaggcg
     5521 tttggtatat actgatgcca gtatcttcct tactctctcc gttattaagc ctgatggttt
     5581 ttatcattgg tacactatac gaattaaggc gaattagtgg ttgtgtaagt gtaagagaat
     5641 ggatgcaatc tcaggtaaat gaacaaacta atgaagattt gcatctggat tttgatgatg
     5701 tgaattttga ttatgtggat ttttatcgta ctaatccggc aacaggatta gctatgcatg
     5761 gaggatttga ttcagcagga aatacttttg ggacttgttg gcaggactat gatgacaggc
     5821 gataagaaaa ctgtggtttg taataaaggt aatttgatga aaacaaaaaa attgatgatg
     5881 gttgcactgg tcagttccac tctggccctt tcagggtgtg gtgcgatgag cacagcaatc
     5941 aagaagcgta accttgaggt gaagactcag atgagtgaga ccatctggct tgaacccgcc
     6001 agcgaacgca cggtatttct gcagatcaaa aacacgtctg ataaagacat gagtgggctg
     6061 cagggcaaaa ttgctgatgc tgtgaaagca aaagggtatc aggtggtgac ttctccggat
     6121 aaagcctact actggattca ggcgaatgtg ctgaaggccg ataagatgga tctgcgggag
     6181 tctcagggat ggctgaaccg tggttatgaa ggcgcagcag ttggtgcagc gttaggtgcc
     6241 ggtatcaccg gatataattc cagttctgcc ggtgccacac tcggtgtggg ccttgctgcc
     6301 ggtctggtgg gtatggctgc agatgcgatg gtggaagatg tgaactatac catgatcacg
     6361 gatgtgcaga ttgcagagcg tactaaggca acggtgacaa cggacaatgt agccgccctg
     6421 cgtcagggca catcaggtgc gaaaattcag accagtactg aaacaggtaa ccagcataaa
     6481 taccagaccc gtgtggtctc aaatgcgaac aaggttaacc tgaaatttga agaggcgaag
     6541 cctgttctcg aagaccagct ggccaaatca atcgcaaata ttctctgatt atcttccgga
     6601 ggctggtctg accggcctcc tgactgacgc ctcgctttac tcgttgtcca accccggaaa
     6661 gaaataaaat aaattttatt tctccccggc gtttccgaaa taaatatgaa aacactgagc
     6721 ggttgtgcaa attctttgtg cgaccgtgaa gtgttgccgg cgaagccgga atttaacggt
     6781 tattaatctt ttcatcatta ataatcagtc cg
//
LOCUS       DBBGLQ010000172         6679 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.172, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000172 DBBGLQ010000000
VERSION     DBBGLQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.172"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            141..1586
                     /gene="tldD"
                     /locus_tag="ACIYSB_004958"
     CDS             141..1586
                     /gene="tldD"
                     /locus_tag="ACIYSB_004958"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGC5431812.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(1720..2649)
                     /gene="aaeR"
                     /locus_tag="ACIYSB_004959"
     CDS             complement(1720..2649)
                     /gene="aaeR"
                     /locus_tag="ACIYSB_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGC5431813.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            2832..3035
                     /gene="aaeX"
                     /locus_tag="ACIYSB_004960"
     CDS             2832..3035
                     /gene="aaeX"
                     /locus_tag="ACIYSB_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGC5431814.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            3043..3975
                     /gene="aaeA"
                     /locus_tag="ACIYSB_004961"
     CDS             3043..3975
                     /gene="aaeA"
                     /locus_tag="ACIYSB_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGC5431815.1"
                     /translation="MKTLIRKFSCTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            3981..5948
                     /gene="aaeB"
                     /locus_tag="ACIYSB_004962"
     CDS             3981..5948
                     /gene="aaeB"
                     /locus_tag="ACIYSB_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGC5431816.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVNRLAGMLHKYQHALTDS"
     gene            6040..6312
                     /gene="yhcO"
                     /locus_tag="ACIYSB_004963"
     CDS             6040..6312
                     /gene="yhcO"
                     /locus_tag="ACIYSB_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGC5431817.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(6368..6631)
                     /gene="yhcN"
                     /locus_tag="ACIYSB_004964"
     CDS             complement(6368..6631)
                     /gene="yhcN"
                     /locus_tag="ACIYSB_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGC5431818.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         1550 a         1656 c         1815 g         1658 t
ORIGIN      
        1 cagccgttgc ctgatgcgac gctgtgcgtc ttatcaggcc tacaaaccgg aacataaccg
       61 taggctggat agggcgttca cgccgcatcc ggcagccgta aaaaatcctc tactgcagta
      121 actaacgagt agcaaaaacg atgagtctta acctggtaag tgaacaattg ctagcggcga
      181 acggcctgaa acatcaggac ttgttcgcga tcctcggtca actggccgaa cgtcgccttg
      241 attatggcga tctctatttt cagtcgagct atcacgaatc ctgggtttta gaagaccgca
      301 ttattaaaga tggttcttac aacatcgatc agggcgttgg tgtgcgtgca atcagcggtg
      361 aaaaaaccgg atttgcttac gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc
      421 aagcggcgcg caccatcgtc cgtgatagtg gtgatggtaa agtacagacg ctgggcgcgg
      481 tagagcatag cccgttgtat acctcggtag atccgctgca aagcatgagc cgtgaagaga
      541 agctggatat cctgcgtcgc gtcgataagg ttgcccgcga agcggacaag cgcgtacagg
      601 aagtgactgc cagcctcagt ggtgtctatg aattaatttt ggttgcggcc accgacggca
      661 cgctagcggc ggatgtccgt ccgctggtgc gtctttccgt gagcgttctc gtcgaagaag
      721 atggcaaacg cgaacgcggt gccagtggcg gcggcggtcg ttttggttat gaattcttcc
      781 ttgccgatct cgacggcgaa gtccgtgcgg atgcatgggc aaaagaagca gtgcgtatgg
      841 cgctggtcaa tctttctgcc gttgctgcac cagcgggcac catgccggta gtacttggcg
      901 caggttggcc gggcgtgctg ttgcatgaag cggttggtca cggtctggaa ggcgacttca
      961 accgccgtgg cacttcagta tttagtggac aggtcgggga gctggtggct tcagaactgt
     1021 gtaccgtggt tgatgatggc acgatggtcg atcgccgagg ttcggtggcg attgatgacg
     1081 aaggtacgcc aggccagtac aacgtgctga ttgagaacgg cattctgaaa ggctacatgc
     1141 aggataaact caacgcgcgt ttgatgggga tgacgccgac tggcaacggt cgccgtgaat
     1201 cctacgccca tctgcccatg ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga
     1261 ccccgcagga aattattgaa tccgttgagt acggtatcta tgcaccgaac tttggtggcg
     1321 gtcaggtgga tatcacctcc ggcaaattcg ttttctccac ttcagaagca tatctgattg
     1381 aaaacggtaa agtaacgaag ccggtgaaag gcgcaacgtt gattggttcc ggtatcgaaa
     1441 ccatgcagca gatttcgatg gttggcaacg acctgaaact ggataacggc gtgggtgtct
     1501 gcggtaaaga agggcaaagt ttgccggttg gcgtgggcca gccaacgttg aaagtcgata
     1561 acctgactgt tggcggtact gcgtaataat ttcactattt tcagaaggat aattaatctt
     1621 tctacgtgca cgaacggtcc cctcgcccct ttggggtgag ggttagggtg aggggaaccc
     1681 gttggcacag gtttgtacaa cgtaacagta caatatgaat tacttctctt tcccgcgccc
     1741 gtgcatctcc tgaaacaatt taccgacctc aacaaaataa tccgtcagcg agttgatcac
     1801 gacctgtacc ttcagcggca gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg
     1861 atctgactgg taacgcggca gcaggatctc cagctcccca cgattgatct cgttgatcac
     1921 ccacatcagc ggcacgtagg cgatcccggc acccgccgtc agccagcgca ccagcgtcat
     1981 cggatcatta gtcacaaatc ttccttgtgg gatcaggcga gtcgagatcc cttccggtgc
     2041 gatcagttca aattcattgt cgggccgcac gctgtattca agccatgaat gactactcaa
     2101 atcggcgggt ttttccggta tgccgtattg tgtgagatag cttttcgcgg cgcacaccac
     2161 cattggcatc gcgcccagac ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg
     2221 gatcaccaca tccagaccgt cggcaatcag gtcgggggct ggaattccgg taaccagatt
     2281 gacgctcaaa cctgggtatt ctttcagcat tttggctgtc agcccggcga gaacattttg
     2341 tgccatagtt gaagaacagc caatgcgtag cgtcccgatg ggggtgttat tgaaggcata
     2401 cagttgctca tgaacatcct gcacttcatg aagcatacga cggcagccct ggtagtaaat
     2461 tctaccggct tcggtcaggc caatgctgcg tgtgctacgg tttaacagct ttacctgcaa
     2521 ctcatcttcc agttttgata ccgtctgact gatggacgaa acgctcatct gtagctgtct
     2581 ggcggcggcg gtaaaagagc caaattcaac tactttggca aacaccgaca tgcgttttag
     2641 tcgttccatt attcactctg acttaaaagt gatttagatc acataatata gataacagca
     2701 taacagttac gctaatatat taaatatcaa tctacagcaa tgttgctctc gcccggcttt
     2761 ccatgccctt ctctgcggcg acagatgctg aaaataataa cgcctgctct ctctttacaa
     2821 ccaaggtcaa catgagtctg tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga
     2881 tattttttga attgctttta tcactggcga ttttctggct ggtgcgccgg gtacttgtgc
     2941 caacaggtat ctacgacttt gtctggcatc cggcgttgtt caacaccgcg ctctattgct
     3001 gcttgtttta tttgatatcg cgactgttcg tttgaggttg aagtgaaaac actaataaga
     3061 aaattctcct gtacggccat cacggtcgta ttagtcattc tggccttcat cgcaattttt
     3121 aatgcctggg tctattacac cgaatccccc tggacgcgtg acgcgcgctt tagcgctgac
     3181 gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc aggtgaatgt tcatgataac
     3241 cagctggtga aaaaaggaca gatactgttc accatcgacc agccgcgcta tcaaaaggcg
     3301 cttgaggaag cgcaagccga tgttgcttat tatcaggtac tggcacagga gaaacgccag
     3361 gaggccggac gtcgtaaccg tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag
     3421 gccaacaacg tactacaaac ggttctgcat cagttagcga aagcgcaggc gacccgcgat
     3481 ctggcaaaac tggatcttga acgcacggtg atccgcgcgc cagcagatgg ctgggtgacc
     3541 aacctcaacg tctataccgg tgagtttatt actcgaggat caacggcggt tgcgctggtg
     3601 aaacagaact ccttctatgt actggcctat atggaagaaa ctaagctgga aggggtgcgt
     3661 ccggggtatc gtgcagagat cacgccgctt ggcagtaaca aagtgctgaa agggactgtt
     3721 gatagtgttg ccgcaggggt caccaacgcc agcagcacgc gtgacgacaa agggatggcg
     3781 actatagact ctaaccttga atgggtgcgt cttgcgcaac gtgttccggt tcgtattcgt
     3841 ctcgacaacc agcaagagaa catctggcct gcgggcacca ctgctacagt ggtggtcact
     3901 ggcaaacaag atcgcgacga aagccaggat tcgttcttcc gtaaaatggc ccatcgcctg
     3961 cgtgagtttg gttaatcacg atgggtattt tctccattgc taaccaacat attcgctttg
     4021 cggtaaaact ggcgaccgcc attgtactgg cgctgtttgt tggctttcac ttccagctgg
     4081 aaacgccacg ctgggcggta ctgacagcgg cgattgttgc cgccggtccg gcctttgctg
     4141 cgggaggtga accgtattct ggcgctattc gctatcgtgg ctttttgcgc atcatcggca
     4201 catttattgg ctgtattgcc ggactggtga tcatcattgc gatgatccgc gcaccattat
     4261 tgatgattct ggtgtgctgt atctgggccg gtttttgtac ctggatatcc tcgctggtac
     4321 gaatagaaaa ctcgtatgcg tgggggctgg ccggttatac cgcgctgatc attgtgatca
     4381 ccattcagcc ggaaccattg cttacgccgc agtttgccgt cgaacgttgt agcgagatcg
     4441 ttatcggtat tgtgtgtgcg attatggcgg atttgctctt ttctccgcga tcgatcaaac
     4501 aagaagtgga tcgagagctg gaaagtttgc tggtcgcgca atatcaatta atgcaactct
     4561 gtatcaagca tggcgatggt gaagttgtcg ataaagcctg gggcgacctg gtgcgacgca
     4621 ccacggcgct acaaggcatg cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg
     4681 ccaatcgacg tttaaaagcg atcaatacgc tatcgctgac gctgattacc caatcctgcg
     4741 aaacttatct tattcagaat acgcgcccgg aattgatcac tgatactttc cgcgaatttt
     4801 ttgacacgcc ggtagaaacc gcgcaggacg tccacaagca gctcaaacgc ctgcggagag
     4861 ttatcgcctg gaccggggaa cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg
     4921 caacgcgtta tcagcttctc aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg
     4981 aagaagagat cctgcaaggc gaaccggaag taaaagtaga gtcagccgaa cgtcatcatg
     5041 caatggttaa cttctggcga accacacttt cctgcattct gggcacgctt ttctggctgt
     5101 ggacgggctg gacttccggc agtggtgcaa tggtgatgat tgcggtagtg acgtcactgg
     5161 caatgcgttt gccgaatcca cgcatggtgg cgatcgactt tatctacggg acgctggccg
     5221 cgctgccgtt agggctgctc tactttttgg tgattatccc taatacccaa cagagcatgt
     5281 tgctgctgtg cattagcctg gcagtgctgg gattcttcct cggtatagaa gtacagaaac
     5341 ggcgactggg ctcgatgggg gcactggcca gcaccataaa tattatcgtg ctggataacc
     5401 cgatgacttt ccatttcagt cagtttctcg acagcgcatt agggcaaatc gtcggctgtg
     5461 tgctcgcgtt caccgttatt ttgctggtgc gggataaatc gcgcgacagg accggacgtg
     5521 tactgcttaa tcagtttgtt tctgccgctg tttccgcgat gactaccaat gtggcacgtc
     5581 gtaaagagaa ccacctcccg gcactttatc agcagctgtt tttgctgatg aataagttcc
     5641 caggggattt gccgaaattt cgcctggcgc tgacgatgat tatcgcgcac cagcgcctgc
     5701 gtgatgcacc gatcccggtt aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca
     5761 cagcagacca tgtgatatct gcccgtagcg atgataaacg tcgtcggtac tttggccagt
     5821 tgctggaaga actggaaatc taccaggaaa agctacgcat ctggcaagcg ccaccgcagg
     5881 tgacggaacc ggtaaatcgg ctggcgggga tgctccataa gtatcaacat gcgttgaccg
     5941 atagttaagt caaaaccgac gccaaaagcg tcggtttttt catggctata cttagcgatg
     6001 cacggcagaa ctcgccgcga aacgtgacgg tggcaacaga tgaatattta tacctttgat
     6061 tttgatgaga ttgagagtca ggaggatttt tatcgtgact ttagccaaac ctttggtctg
     6121 gcgaaagata aggtacgcga tctcgactca ctatgggatg tgttaatgaa cgatgtcctg
     6181 ccgctaccac ttgagattga atttgttcat ctgggagaga aaacgcgtcg ccgttttggc
     6241 gcgttaatat tgctgtttga tgaggcagag gaagagctgg aagggcattt gcgttttaat
     6301 gttcgtcatt agcgcaaaaa aaagcccccg aaccgggggc aatatcgtcg gacaagacga
     6361 tgagggttta tttgtacagt tcagccgtag cgtgccaggt gtcaccgcta cgagcttcag
     6421 taatctggta ggccgttgcg cctttctctt ccgctttttt gttcagcatt tcacgcatat
     6481 ccattggcga agacgccaca ccacttacgg atacggtccc gattgcttca cgattttgtg
     6541 cttgtgcagc atcaatggag tcggcagcga atgcaccgaa agagagaaca gaaagtacgc
     6601 ttaatgcagc aacagtggtt ttgattttca tgatttttac ctcgacataa tcttttagct
     6661 gggcctttgt ttcgtgacc
//
LOCUS       DBBGLQ010000173         6662 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.173, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000173 DBBGLQ010000000
VERSION     DBBGLQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.173"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..87)
                     /locus_tag="ACIYSB_004965"
     CDS             complement(<1..87)
                     /locus_tag="ACIYSB_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431819.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMEGLFQRF"
     gene            complement(1297..3120)
                     /locus_tag="ACIYSB_004966"
     CDS             complement(1297..3120)
                     /locus_tag="ACIYSB_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431820.1"
                     /translation="MRMFTNLLYDICTVFELFKEGESPRDKRKSTDFGAHQRFWDQRY
                     NELSHIIDAEGVYSLEQRRIIFSRYEYFYYMMNSYPVYSTLKSEYIRNYFLKSFGVVF
                     IVLDIYNTYRPENETGFYYHIYNFLQKSYCPCLDYSGTESDEAAVKRYLREYLAELGF
                     NREDFRENGKMYELGKYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELD
                     RILNNIDKFYYAFYSLSILLDMQRKVKILDSIAYYLRVLIREGLWVHGLYGYAARYLY
                     DFNIFDTTPYARALLERFHEFESGPKGALTRYIVSLDDKSQEYIESLKDMVFNLSDKK
                     SYDDVYLENIINYFEQLQNARGYVTRCYMLLAVLIYLIRRNKLHKALRFYDESPKYEL
                     PSGYLPGAFSVLRIALEIKLNREKIKHGSLFELLDYVKAYQDAFMDLRVVTDPAYNED
                     EIQYDANNFTLMRVIKMYNSMLANISTKSDIQPPYITGLLDNVERALDKINILIDKER
                     VYDGETLAELITENKILSSRESKENLIGLFTGRHKYTLLQCIEKLGVLVDYVISPVDD
                     IKNVMMLYGNNAENKNRRRLIYNALTIICGDDTKNNQSDPR"
     gene            3221..3469
                     /locus_tag="ACIYSB_004967"
     CDS             3221..3469
                     /locus_tag="ACIYSB_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5431821.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            3485..5050
                     /locus_tag="ACIYSB_004968"
     CDS             3485..5050
                     /locus_tag="ACIYSB_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HGC5431822.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSVMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACAKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRIRMTLSHAASHDLEIFHEEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDKWLRENIIRVKGYEYQKSHILQYGPYALRNKCVLDEAL
                     DILAEQKKIVIDYSIGQKIIYIGDAITPCELANEANIPIMERGMFIVCWDHKLNKYRN
                     EEQTKIWNITTKN"
     gene            complement(5274..6482)
                     /locus_tag="ACIYSB_004969"
     CDS             complement(5274..6482)
                     /locus_tag="ACIYSB_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5431823.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
BASE COUNT         2101 a         1522 c         1189 g         1850 t
ORIGIN      
        1 aaaacgttga aacaatccct ccatgtgttc attcacatct ttcagaatcg cgaaattcat
       61 ttcgtaattg ggattggcat tatacatttt atagtcctta atttataaaa ctcacaggaa
      121 gaggaaatta tgtaaatact gagagaacat aaacgccgta ccacggcatt aataaacgaa
      181 gtaaaccagc agcacagaca ccctgataaa actgttttgt attaccgcat cgctgtaacg
      241 aggtaatgct tcagaataca gtgaggctct cttaaacaga ggaccagtaa gaattacaat
      301 ggtaaaaaca cacctcatag aattgagttt aaggaagcac cattcactca acctgacatt
      361 cacaaaagtc agtaccaggt ggcctcaggt aacatcgcta ttaatattat cctgccatta
      421 agtaaaaaca gaaactctgt gaggaagtct caccaatatt tgaccagtag cataaagaat
      481 aacgatatga gagatacgcc cacaccagag atgattaccc catacaaaaa gataatattt
      541 attaatacca ccgcacctca atagactaat aacacactaa tagcgttttg gtacacactt
      601 aacactaact atgcaccaga cacatactca ggattaacat tcccaacatt tatggtactc
      661 tcaaagagta taattcacca cacaggtaaa ctctcacacc agtagtatgg agataatgct
      721 gtacacatac ttacagagta aagtgtacag ttagcataca gtggcgagca gacattacaa
      781 cataaagaca taagatagca taataagaac ataacaaaac tcccactaat aatactaatt
      841 aaccttaatt cttttttgaa caggaaatca acattgctcc agacctcttc aggtcataca
      901 cttttgtatt ggatgttcaa tgtgtacttc tgcacaaaca aaaaaataaa acccgcaaaa
      961 taaaatatca gccccaaaaa tacagcgtga aacaccacgt tatggatatg ttttcatatc
     1021 accagaagca tatttcacac atacaataca gaaacaattc atcatataat cagggttact
     1081 gttcactcct ctcggttatc cgcgagacga taaaacatga cactaatcac aaaaacgaga
     1141 gatagtattt gcgaaatata aacaacataa aaatcaatgc gttgcgattt tttatttttt
     1201 cagacatgca tgccccctct gctattgttg ctagccggct agcaacaata gcagggatat
     1261 acaaacatcg gatgccaaaa acacgttccg ataatatcat cgaggatctg actggttgtt
     1321 tttagtatca tcaccacaaa tgatggttaa tgcgttgtag atcagtctgc gtctgttctt
     1381 gttttcagca ttatttccat acaacatcat gacgttttta atatcatcaa ccggtgatat
     1441 tacataatca accaatacgc caagcttctc aatacattgc agtaaagtat atttgtgtct
     1501 gcccgtaaat aatcctatca ggttttcttt actttcacga ctggataata ttttgttctc
     1561 tgtaattaat tcagccaacg tttcaccatc gtaaacacgt tctttatcta tcagaatatt
     1621 tatcttatct aacgccctct ctacattatc aagaagacct gtaatatacg gaggctgaat
     1681 atctgacttt gtactaatat ttgcaagcat cgaattatac attttaatga cacgcatcag
     1741 agtaaaatta ttcgcatcat attgtatttc atcttcatta tacgcaggat ccgtcacgac
     1801 ccttaagtcc ataaatgcat cctgataagc ttttacataa tcaagtaatt cgaacagtga
     1861 accatgcttt attttctccc tgtttaattt gatctccagc gctatcctga gaactgaaaa
     1921 agcgccggga agatacccag aaggtaactc gtattttggg gattcatcat aaaaccgaag
     1981 agctttgtgg agtttgttcc gccgaatcag ataaataagg acggccagta acatgtagca
     2041 cctggtcaca taaccacggg cattctgcaa ctgttcaaaa taattaataa tgttttccag
     2101 atatacatca tcataacttt tcttatcaga aagattaaac accatatctt ttaaagattc
     2161 gatatattct tgtgatttat catcaagtga cacaatataa cgagttaatg ctcctttggg
     2221 gccactctca aactcatgaa accgttcaag taatgctctt gcataaggcg ttgtgtcaaa
     2281 aatattaaaa tcataaagat agcgtgcagc gtagccatac aacccatgta cccaaagccc
     2341 ttctcttatc agcactctga gataatatgc aatactgtca agtatcttta ccttccgttg
     2401 catatcaagc aaaatactta atgaataaaa tgcataatag aacttgtcaa tgttgttgag
     2461 tattctgtcc agctcactct tatcaagctt cttctctctg taatccttct tgtattcatt
     2521 cttacaggcc tttatatatt gtttcataag gctcttcctt ttaccgtacc cttttcgaat
     2581 agttccctgg tattttccca gctcatacat ttttccgttt tcccgaaaat cttctctgtt
     2641 aaatcccagt tcagcaagat actcccggag ataacgttta acggcagcct catcgctttc
     2701 tgtaccagag taatccaggc agggacaata tgatttttgc aaaaaattat agatatgata
     2761 ataaaaccct gtttcattct ccggacgata tgtgttatat atatccagca caataaaaac
     2821 aactccaaag cttttcagaa aatagttacg gatatattcg gactttaatg ttgaatacac
     2881 agggtaagaa ttcatcatgt aataaaaata ttcataccgg gaaaatataa ttcgccgttg
     2941 ttccagacta tacacccctt cagcatcaat aatgtgactg agctcattat aacgttgatc
     3001 ccagaatctt tgatgcgctc caaaatcagt actcttccgc ttatctctcg gactctctcc
     3061 ttccttaaac aactcaaaaa ctgtacaaat atcatacaaa agatttgtaa acatgcgcat
     3121 ctgctctgat ctccggttgg tttccgtttt atcacagggg acattctggc cttaacgtag
     3181 caaattaatg acatagcctt caactcacat ggagaacaat atgtcaaccc agaccggaca
     3241 tttttatacc ccctcacgca tcctgcgtat ggctgaactt acccgactcc ttggtatatc
     3301 aagatcgacc atctacgaaa aaatgaatcc ggcatcaaaa tactacgatg ccacctttcc
     3361 ccggcctgtc cggcttggga gttcggctgt aggctggcgg gcatctgcca ttgatgagtg
     3421 gttaacttta catacaacac caaccagatc cgtaactaag gataaataag gaaaactgta
     3481 cgccatgaca aaattatccc cccctgaact tgacataact aaactgcctg acaggtgcca
     3541 ggcattactt gatgaaatgc atgaagaaac gggaataagc cgtgaaatac tgctgtctgt
     3601 tatgctgacg gtcaaggctg cctcggtcca ggatacgcat gaagttgaac tttctggagg
     3661 gcagcgtacc agtcttcaga tatatatgtg tctttcatca gcatcaggca gtggaaaaac
     3721 ctctgcctgc gcaaaattaa tcgcccctgt tcatgaaaca gaagaagaac tacatcaggc
     3781 atatatcgac gataaaaaaa actatgatcg catgatggaa atgtggacaa cagataaaaa
     3841 aatcctggag cggagatata aaaaggaaat ggaaagatcc ccggaaaatg ctgctgctgc
     3901 acgagcagcg cttgaaaagt gtatagcaaa taaacccgta cctcccgtac aacaggttct
     3961 catcgtgaat gacgcgacac cagaagggat agctttgaaa ctcagccagt caccttctct
     4021 tctgttgctg tctgatgaag gaggaactat tctggataag cgtttcgaac gcaaatcggc
     4081 gctgtacaac acattatgga gtgggcaacc agtcaccgta gaacgggcat ccagacccgg
     4141 gttccgggta aaggattccc gtctcactat gcttatactg actcaaccgg taatatttga
     4201 taagtttttt actctcactg gcgaccagat ccggggcaat ggctttctgg cccgggtgct
     4261 attttgcgaa cccggagcaa ataaaataat gacgacagaa ccgcatactg ccactcctgt
     4321 tgtaacgcag caatgtgcaa cctgttttca tgagaaaagt tttggcagtc agatcagaga
     4381 ctctctgaga gcgtcccggg aaaggcgtgc aaaaggtgag caacgcatcc gcatgacatt
     4441 atcacacgca gcatcccacg atctggaaat ctttcacgaa gaaaatatga gtgctgtcag
     4501 gcagaaccca cgtatgacca ctttcgaaga cattatcgtc aggaaaagag aacaggctgt
     4561 tcgaatagcc gcacttctgg agctggagaa caatccacat ggtacagtaa taacacgaga
     4621 aagtatcaat agtgctattt atctcgttga tttttatttt cagcatctta tatccaaact
     4681 agaatcactt cgggaaatat ctcctgcaga aaaactggat aaatggctca gagaaaatat
     4741 catccgggta aaaggatacg aatatcaaaa aagccatata ctacaatatg gtccatatgc
     4801 tctcaggaat aaatgtgttc ttgatgaagc acttgatata cttgcagaac agaaaaaaat
     4861 cgtaattgat tattcaatcg gccaaaaaat catttatatc ggtgacgcga ttacaccttg
     4921 tgaattagca aatgaggcaa acatcccgat aatggagcgt ggaatgttta tagtctgttg
     4981 ggaccataag ctgaataagt acagaaatga agaacagact aaaatatgga acataactac
     5041 aaaaaattaa agttcccgtc ttttatcatt cagttaccaa cctgaatatc tgaaacaatg
     5101 ccagacatcg gttacaaact ggcattgttt ttatcattca atttataaac tacaatatta
     5161 cgtattattt ttacatttat gtcattgcaa atatcagaca aacaagcatt ataactcaat
     5221 aagtatattt ttcgtgatta cagacgacat ctgcaaatct gtgagatgtg atttcatcgc
     5281 tggttttcca taatctgaga catcacataa tccgcccatg cctgcatcat cggtatacgc
     5341 tgatccagca gatcagtccg gtgatatgcc gcctcaacct tatttttcag cgtatgggca
     5401 agcgcccttt ccgccaaatc ccgcgaatat ccctgttcgc tacaccagtc cctgaatgtt
     5461 gaacgaaaac catgtgctgt agccactcgt ccgggtatat cactgatggc cttcttttta
     5521 cgcagaaaac tcgtcaacac catatctgaa aggatctgct gctttctggg agaaggaaac
     5581 accagttcgt catgcagacc acgtatattt tccagaacgt gaatggcctg tcgggataaa
     5641 ggaacacgat gctgtatcct ggctttcatt ctttctgcgg gtatcgtcca tatccgctta
     5701 tgaaaatcaa tttcagccca gcgcattccc cttgcttcgc ccgagcgggt tgctgtcagt
     5761 atcaccatta ataacagtgc ccgggtaaca ttataaggtt catctgtata cacactggtc
     5821 gccacaaaaa gcggtaactg cctccagggc attgccggct ggtgttcatc acgtcctctt
     5881 gattgctgtg gaagcaaatg atcaaccaca tcaacaggat tcgccacaca aaaaccatgc
     5941 gcccagcccc actgcataac aacatgaatg cgctgtttaa cccggcttgc cgtttctgac
     6001 aaggttaacc agactggacg cagtgtttct gccacgtccg cagccgtaat cgaatccagc
     6061 gtttttgctc ccagttgagg aaacgcgtaa ttctcaagcg tcgataacca ctgccttaca
     6121 tgctttggat tttcccatcc aggagacagt tctgcatgta cacgcctggc tgcatcggca
     6181 aatgttggga taacaacttt ttcagcttca gcctttttaa tctccagagg atcatcacct
     6241 gcagcaagtt gctctcgcat tatccgggca gtacgtgcag cttcagcaat actgacctca
     6301 ggataagttc ccaatccagc attacgcctt ttttgtgtca ccggacttac ataacgaaaa
     6361 acccatttac ccctcccctt tactgaagaa ggatgaagag tcagtcctgt aattccccca
     6421 tggggcaatg gtttgtcatc aggtttgata tgccttgctt tagtatccgt caatactgcc
     6481 atacgctaat tcctgttact ctggtatgcc attcagtatg ccattagtct ctcacttcac
     6541 taagaataca ttagataaca tcggacagca aaaattgtaa tgcattgttt ataatgaggt
     6601 tatataacac tcccggatta agtcagattt atttcaggcg gtccccctca ccgccatatt
     6661 ta
//
LOCUS       DBBGLQ010000174         6610 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.174, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000174 DBBGLQ010000000
VERSION     DBBGLQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.174"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(200..418)
                     /locus_tag="ACIYSB_004970"
     CDS             complement(200..418)
                     /locus_tag="ACIYSB_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431824.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRD
                     FGNTGECIEVKTNEPLNNVMETLNGGGQ"
     gene            complement(498..974)
                     /locus_tag="ACIYSB_004971"
     CDS             complement(498..974)
                     /locus_tag="ACIYSB_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431825.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQSLLQLMRLTEDVPDA
                     RQELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEENKSE"
     gene            complement(967..1188)
                     /gene="traR"
                     /locus_tag="ACIYSB_004972"
     CDS             complement(967..1188)
                     /gene="traR"
                     /locus_tag="ACIYSB_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HGC5431826.1"
                     /translation="MSDEVDEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(1323..1838)
                     /gene="traV"
                     /locus_tag="ACIYSB_004973"
     CDS             complement(1323..1838)
                     /gene="traV"
                     /locus_tag="ACIYSB_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HGC5431827.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTITATTPSGSRPAVTTLPEQKLLTPR
                     PLFTAAREVKTVVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(1835..2155)
                     /locus_tag="ACIYSB_004974"
     CDS             complement(1835..2155)
                     /locus_tag="ACIYSB_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HGC5431828.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKEFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(2142..2732)
                     /gene="traP"
                     /locus_tag="ACIYSB_004975"
     CDS             complement(2142..2732)
                     /gene="traP"
                     /locus_tag="ACIYSB_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HGC5431829.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRGNGDKNEHA"
     gene            complement(2722..4149)
                     /gene="traB"
                     /locus_tag="ACIYSB_004976"
     CDS             complement(2722..4149)
                     /gene="traB"
                     /locus_tag="ACIYSB_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HGC5431830.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(4149..4877)
                     /gene="traK"
                     /locus_tag="ACIYSB_004977"
     CDS             complement(4149..4877)
                     /gene="traK"
                     /locus_tag="ACIYSB_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HGC5431831.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAGTGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(4864..5430)
                     /gene="traE"
                     /locus_tag="ACIYSB_004978"
     CDS             complement(4864..5430)
                     /gene="traE"
                     /locus_tag="ACIYSB_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HGC5431832.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(5452..5763)
                     /gene="traL"
                     /locus_tag="ACIYSB_004979"
     CDS             complement(5452..5763)
                     /gene="traL"
                     /locus_tag="ACIYSB_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HGC5431833.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(5778..6137)
                     /gene="traA"
                     /locus_tag="ACIYSB_004980"
     CDS             complement(5778..6137)
                     /gene="traA"
                     /locus_tag="ACIYSB_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HGC5431834.1"
                     /translation="MNAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGL"
     gene            complement(6170..6565)
                     /gene="traY"
                     /locus_tag="ACIYSB_004981"
     CDS             complement(6170..6565)
                     /gene="traY"
                     /locus_tag="ACIYSB_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-bindin protein
                     TraY"
                     /protein_id="HGC5431835.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
BASE COUNT         1603 a         1803 c         1490 g         1714 t
ORIGIN      
        1 cttccttaaa tgcttctgct gcacagggga tatcctgtgt aatctctgtc agttgttctg
       61 gtgttgcagg ttgttgctca aggtagtcat tgtaaaaaag accacatatc aacatagcca
      121 taacgccaac aaatataaaa gccagaacag attttaaata tatcgacttc atttcatttt
      181 ccttcgaagc agtaagcact cattgcccac caccattcag tgtttccatc acgttgttca
      241 aaggttcatt tgttttaact tcaatacatt cccctgtatt tccaaagtcc ctgcctccgt
      301 gtgcttcgat tgtccgaaga acctgcacgc atacgtccgt atttggcatg ggagtaacat
      361 tccagcttcc gccaccgtta atatttttta tcagcacaag aaaaataaag gtatgcattc
      421 tttattaccg cctgttaaat attaatcact gaatatccct tcagttaact tatgaggtca
      481 taaaacactg cagattctca ttcggattta ttctcttcag taaacttccg gatggtatcc
      541 tgttcacgct gacgggtggc agactcctgc attgcatcca ctttctcctg ccagagacgc
      601 cggatatcct gttcatcccg accagtcagt tttttaccgg acaacaaacg gttaagcagt
      661 tcctgtctcg catccggtac gtcttcagtc agacgcataa gctgaagcag gctctgacgg
      721 ctgaggacca tgcgtgattc ctccggtacg ctgtaataat taaaaatacg actgaatacc
      781 ccacaacagc aggcaacgga caggcacagg gatttcagat acaggccaga aacacagaat
      841 gtggcaacac cacaaagaat agctataact aaaaagccct tacccagcgc catacacaaa
      901 gaaccctgtt ttctggatat ccgctcatgc tcatccagaa taaaatctat tttttcattt
      961 tctgatttat gcatagtgtt tcctctgcct ttcctgatac gcctgacatt caacgcacaa
     1021 cgtcacaccg ggaaatattt tccgccgggc ttccggaata ggatttccgc atgcttcaca
     1081 gagataaaca ggaataccat gagcatttat tttctggcgt atccggttta ttcctgtcat
     1141 ggtcagttgt tctgtcactg aatatgcttc atcgacttca tcactcacgg aatattcctc
     1201 ccgacatgtt ctgacacgct accgcccatc ccttttcacg cacagggcac tcgttttaca
     1261 ggcagatttt gaatacagaa agcccgccgg cattgccggc tgatatacaa cttcagggcc
     1321 agttaattaa tacgtggttt tccccacgca gacggtttaa taacaaaaaa cacgctggat
     1381 ggctgatgat aaacatcctg attatcaata taaggcgcta tccataatgc tgccgtctgc
     1441 tcacccgttc ttagcggacg gggaggcgtt accggcgcaa ctgaactgac cgggacaacc
     1501 gttttcactt cccgggctgc agtaaacagc gggcgaggtg tcagcagttt ctgctccgga
     1561 agtgtcgtca ccgcaggtct gctgccggac ggagtggttg cggtgatggt ctgcaccggc
     1621 attgtccgga agttcccttc agccaggcgc ggcagtgatg ccgcaaccgg ctttgcttct
     1681 gaggactgct ccagtttttt ggccttctca ttggcctgct ccatcgtcat acaggtatcg
     1741 gatgtggtgg cgttacactc aaattccgta tttgttcctg cgcagccgga aagtaataag
     1801 gttcccagca gaggaataaa taaagaaatc tgtttcattg cccttctcct tttttattac
     1861 tccccacaac agcagaaatt aactctgctg tcagatttgt tttccctgca cagggatgtc
     1921 cggagaccac tgtatttccc ggagaagaaa ataagctcat cgacattccc ttcttcatca
     1981 caccgacagc agccatatta aaattgtgcg gcaatgattc agaactgaac tccttcggag
     2041 caggaagaac tatttttgtg gtgcagttac tgagaacagc atgcataaaa tcatctgata
     2101 cgtttttatc cggcacaacg cgggctgtaa taacattaat attacgcatg ttcattttta
     2161 tctccgttgc ctctgtttgt tcggtcatca gggaaataaa catggcattt cctgattttt
     2221 cccggcaggg aagcgtcttc cgtataaacg tccttcagat attcatctgc ggttaccacc
     2281 cggatacatt tcatttttcc gggcagcacc ggatatttcc cggtgatcgc tgccagcgag
     2341 acatacatca gtgccagtgt tgcccataat gcggtgacca gttgcatcag tgaccggtca
     2401 gtctccctga cataatctgc agcatcagcc gcgcggtaat ggcagatatc ctgtatttct
     2461 gatctgcctg aagaatcaga ggaagaaaac cagcattcac tgaccgggaa ctgtccggca
     2521 ggtgctgttt gtcttacgaa gttaatctcc ttaaccagta atttccccgg cgtggtgttg
     2581 tccttccata aaacaaacag agccatcagg gccattgtgg ccagtggcag aatgacggtg
     2641 tacttcagac accagaacag tcctcgcaga acccgggcga caacataccg ggcggcatgg
     2701 cacgcctgac gggacgacat attatttgcc atcgttgccc cctccctggc tgccggacat
     2761 aaactgctgc ggattcaggt tgcccagctg cttcagcatc tggtcagtat taaagccgtt
     2821 aagatgactt tcagccgatg gcggaacagg aaccggactt tccggattgt cttcttccgc
     2881 tctgctctgc gtgcgttcca gcgccatctc ttccacggtt ttcagctgga agccgtcctg
     2941 gaacaccacg gtcacttcgt tgcccgcacc aatcggtatc accgggtgat actgttcggc
     3001 acgtttgatg tagtagtcac tgagcgtctg tgcggctttc gatgcgccgc caccgatacc
     3061 cattttcagg acatcaccag ccccgtaagc ggctgtggca cccagcccga cagccggctg
     3121 ggaagcgcgc tccattccct gaccgatacc gtcaacaaat cccgcgcccc atgcccagcc
     3181 gaggattttg ccgttacgca tcaccacttc gcccttgata ccgtttttac cccggaagct
     3241 gacatgcccc ttaatcggca tatcaatggt tttgccgtcc ttcaggcagc tgatattgcg
     3301 ggtgcgtaca atggcacgtt cactggacac atccccccag gcttccagcc ccacaaaaca
     3361 tcccgttgcg tcatacgttt tgctgttcgg catttccacc agaccggtga tacgcagctg
     3421 catcggcacc gtggattcat taccggtgac tgaggcattg gcatccgctc cttcaatcag
     3481 catggctttc gcaaaacttc ctgacggaat gtacggcagt gatggtccct gttttccctc
     3541 attacgggtg aacaccttac gctgtatccg gttaggcacc ggtacagact ggtacgtcac
     3601 ctgcggtggt ggcgtgacac cattacctgg ataaaacgcc gtcggcggtg gaacagcaac
     3661 gctgccctgc ggcgggaaag atacgggtgt gtttcctggc tgtggttcgc cttccgggcc
     3721 gggcggtgag gctggcgtct gcggtactgg ctcaccggtc gcggtgacag gatttgcacc
     3781 cagggctttc acctgctctg ccagtgcagc attgtcctgt cccagctttt caatacgacg
     3841 ctggtcatca ccgcgctgtt tgttcaggac atccagctca cgacggattt cctcatactg
     3901 cttctgcatc tgtgctgccg tcacctgcat ctctgtggtg gcatgctgac gcaccttgtc
     3961 atcaaaggtc gtatccacca caccggtcat atccggcaca ggctcctgtt cagccacggc
     4021 ttcaccgtta ccggacatgt ccacatcaga cagatacagt gccccaccaa tcgcggaggc
     4081 tgcaccgaca accacaatcc ccagccacag gtactgcttg cgtttcacaa tggtattgat
     4141 actggccatc agttgccctc cccgtcacgg atgacataca catccatgcg cgcacctgcc
     4201 agtaactggc tggcaggctg gctgaacatc acggcacggg ttcccggctg ccagaaatca
     4261 ctttcccgga tattcagggc agacagcgtt ttattttcca cggcaaagcg gaccatcttc
     4321 aggtgattcc ccgtccatac ggcatctgcc actgaagaca gccccgccgg ggcctgcagg
     4381 gtttcctttg tcaccgggac ctgataccag cctgcgggta attttccgcc acggacggcc
     4441 tggctgatgg tcacaagcag ggattcgtaa ggcgtggacg tttcccacgc accggcttct
     4501 tctccggttc cgcgcaggtc actgaccagc tgaatggtac gccccgcgcc ttcacgggga
     4561 acggcctgaa tggaaagatt cagaccacgt tctgtttcca gaatgaacgt aaagggcttt
     4621 ttgttgacgg tggccaccac cacaccgccg gaggcggtct gctcattatt ggtcagggca
     4681 ccatccagac tgttcacggc gataatccgg tcaccgggaa cggtaaacag attcgggctg
     4741 gtattgctga tggcaatact ggcctgacca ccgttcacca ttggcaccac ggtgggggcc
     4801 agcgtacctg taccggccat cacgctgcag gaaaaaaaca ggctgccgaa tattattgcc
     4861 gtattatttt ttctcatcgt ctgtttcccc gaaattatcc agccaggtca ccccgttttc
     4921 ccgcttcaga ataaggatgt aatgtttgat atcagtgaaa ggttttgaat caccaatcca
     4981 ggttttaagc acaccacgaa tttccacacg gccatactga ggccagacac gaacactggt
     5041 ctggaaaaag gctgagttca cgttatcgtt tttaatacgc ttcgcttctt cagccagaat
     5101 aactttcatc tggttctgtg cgcccgggcg gatatattgc agaagcgcct gatgtgaggc
     5161 atcgacggtt tctgatgaaa cattcagacg gagggcaata aatgacagcg ccatctgctg
     5221 taaataagag gcgtcggcac tgttctgtga cacggcaaag ggagcattaa atgccattgg
     5281 cgtcacggca gtgcgttgct cattctgaag acggtagtta ttcaccccct gaatgacgtt
     5341 aacagagagg ctgagaacaa taagcactga cataaatata aaggcgatgg ccattacacg
     5401 actggtactt aaacgggcac cgtgttccat ataacaatcc tggtatcagt tctatttaat
     5461 ccactgccgg aaacacgaat cgggaacatt atgaaaaata ccgcgcagca gggctgttgg
     5521 catataccag taaatcaggt cacgtaacca ggaactgccc cgcccttttt tcagtttttt
     5581 aatcccgaaa taaaccagaa ccgctgcacc aataccgaac agatatttcg atgttgtgat
     5641 accccagcca atacagattg ctgcggggat cagttcatcc agtggcaggc caaaccagcg
     5701 gctctggttg gtcagtgttt ccgggaaacg atatttctta agtttattct cgtctcccga
     5761 catcgtttta tttcctgtca gaggccaacg acggccatac ccacagcaat aaatacagag
     5821 atgatggcaa aaccggccag gaacttgacg tttttggtca tcatgtacat gacagcaccg
     5881 accagaactt cagccagaac aacccattta acaacactgg agtccttacc gaaggtcgcc
     5941 ttaaccgtgg tgttaccgct tgccatcagg tcctggccct gtgctgccat tgccagttgc
     6001 gggaagaaca tcatcagaac agcagccggg atcacagcgc gagccaggcg aagcatattc
     6061 agacgagtga atttagaaaa aaacgatttc tttttgacgg gcgcagaagc accctgaaca
     6121 cttaaaacag cattcataag ttacctcaat ttcggataaa tgaataaaac tagagtgtat
     6181 taaatgttat atcttcctct ttatctgcct ccctgaatat ctccgagtta taaaaatcag
     6241 gaaacttgat caaatgatca ataactctgt ctgcagcctc atctgtttta caccatccac
     6301 tacgattttt ggctgcaata agtagctggt tagtttcatc atccagacgc attgtcagat
     6361 acttaaccag cgacttacca tagatattcc ctgccctgtt aaactttgga tagcggtgaa
     6421 gatgatcttt aagtcttatt actgcctcaa acgatcgact tcttccgctt ctgttacttg
     6481 cctcaattaa tagactttcc acatctacag gtaattttag ttttaccatc ttacctgttg
     6541 cagaacgtgt accaaatctt ttcaataaca cctcccgctg tttatcttct ggttaccact
     6601 tatgtttgca
//
LOCUS       DBBGLQ010000175         6517 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.175, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000175 DBBGLQ010000000
VERSION     DBBGLQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.175"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..140
                     /locus_tag="ACIYSB_004982"
     CDS             <1..140
                     /locus_tag="ACIYSB_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431836.1"
                     /translation="RIDLRSKYYVKPKADHPWLTRRTQSHQQVKPPKLPKKKPDPDKK
                     D"
     gene            complement(577..909)
                     /gene="flxA"
                     /locus_tag="ACIYSB_004983"
     CDS             complement(577..909)
                     /gene="flxA"
                     /locus_tag="ACIYSB_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGC5431837.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(1112..1417)
                     /gene="ydfV"
                     /locus_tag="ACIYSB_004984"
     CDS             complement(1112..1417)
                     /gene="ydfV"
                     /locus_tag="ACIYSB_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HGC5431838.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            1442..1681
                     /gene="relB"
                     /locus_tag="ACIYSB_004985"
     CDS             1442..1681
                     /gene="relB"
                     /locus_tag="ACIYSB_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HGC5431839.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            1681..1968
                     /gene="relE"
                     /locus_tag="ACIYSB_004986"
     CDS             1681..1968
                     /gene="relE"
                     /locus_tag="ACIYSB_004986"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HGC5431840.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            2040..2195
                     /gene="hokD"
                     /locus_tag="ACIYSB_004987"
     CDS             2040..2195
                     /gene="hokD"
                     /locus_tag="ACIYSB_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGC5431841.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2412..2663
                     /gene="rem"
                     /locus_tag="ACIYSB_004988"
     CDS             2412..2663
                     /gene="rem"
                     /locus_tag="ACIYSB_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HGC5431842.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            2730..3008
                     /locus_tag="ACIYSB_004989"
     CDS             2730..3008
                     /locus_tag="ACIYSB_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431843.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            3010..4059
                     /gene="ydfU"
                     /locus_tag="ACIYSB_004990"
     CDS             3010..4059
                     /gene="ydfU"
                     /locus_tag="ACIYSB_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGC5431844.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            4073..4825
                     /gene="ydfT"
                     /locus_tag="ACIYSB_004991"
     CDS             4073..4825
                     /gene="ydfT"
                     /locus_tag="ACIYSB_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGC5431845.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(5103..5192)
                     /locus_tag="ACIYSB_004992"
     CDS             complement(5103..5192)
                     /locus_tag="ACIYSB_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431846.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(5247..5459)
                     /gene="cspF"
                     /locus_tag="ACIYSB_004993"
     CDS             complement(5247..5459)
                     /gene="cspF"
                     /locus_tag="ACIYSB_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HGC5431847.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            5760..5975
                     /gene="cspB"
                     /locus_tag="ACIYSB_004994"
     CDS             5760..5975
                     /gene="cspB"
                     /locus_tag="ACIYSB_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HGC5431848.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
     gene            complement(6339..6509)
                     /gene="ynfU"
                     /locus_tag="ACIYSB_004995"
     CDS             complement(6339..6509)
                     /gene="ynfU"
                     /locus_tag="ACIYSB_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HGC5431849.1"
                     /translation="MSERKNSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
BASE COUNT         1774 a         1350 c         1606 g         1787 t
ORIGIN      
        1 cccgtattga tttacgctca aaatactatg ttaaacctaa agctgaccat ccctggctta
       61 cgcgccgaac gcaaagtcat cagcaagtta agcccccgaa gttacctaaa aagaagcctg
      121 atcccgataa aaaagattga aaccaagatc gattcggttg agtgcatatc cattcatagg
      181 gtagattctt aagtcgcgtt tctggtgttc attttcgggt ggtttgttac ttgttttacc
      241 ggggatatgc cagaaacgcg ctgagtcagt ctgggcggtg cgcgtaatga ggcgttatgg
      301 taaatagcct atgctaatgt ccgctaagag caagaagcgg aagttggcag ttttgtggac
      361 tgtccccaca aaagtgacta cagaaatagt tgcaattcat aattgatcat gggttgtcag
      421 ttaaactcgt ggcgatttaa atagactaat tgggagtgcg tccattactt atatcttgta
      481 atgttaacta tcagaaatga tacaaagata atatgtcttt aaagaaaagg ctgatggcga
      541 aaagtggccc gatgagggcc acaatacggc tgtcacttag acgtaaatat caatggtgcc
      601 agcggtattt gtatcgtctt ttttctcttc ttttttatca ggctgaactg tcgcgtcttc
      661 attctttttc tctgcctgct gccttaacaa ctgctccagt tgagcccaga ggctttcaat
      721 ttgcttctgt accaatgcag ccatttcttt tttctgctgt gtcgtcatcc cctcttccga
      781 tgagattttc ccaagctttt cagtcagcac ctgaatttgt cttgtgattt tggctatttc
      841 tgatgttcct tccggggcgg agttgtttga aataacggtt gaggtatttc cctgaattgt
      901 gacagacata gatttctcct tttaaaaaag cactatcggc atgcacaaaa aaatctttaa
      961 tcgtatttct tgtgtcatta attgtttgat gttcagattg ttttcctcgc gggctggcgc
     1021 gcctcagaaa gtaaagcttg ttgacagggg taaacgttcg gcaataattt tctgccgcat
     1081 gcgggtgttg cataaaacgt gttacgttcc tttatcgaca ggtcaggtca ccgctcaccc
     1141 gccgacgaga aagcaacact gacatgctaa agcaaaaaat agatgaataa gttgagttgt
     1201 gcatatgtag cctgaccgtc acaaagtata tggtgtctgt accagtaaga tgatggccgg
     1261 actctttaaa aacgagctga cctgcacaat acaggatgga cttagcaatg gctgctcctg
     1321 gcacaaagcg gacagtgatc accgttctta cgactacttt ctgacttcct tcgtgacttg
     1381 ccctaagcat gttgtagtgc gatacttgta atgacatttg taattacaag aggtgtaaga
     1441 catgggtagc attaacctgc gtattgacga tgaacttaaa gcgcgttctt acgccgcgct
     1501 tgaaaaaatg ggtgtaactc cttctgaagc gcttcgtctc atgctcgagt atatcgctga
     1561 caatgaacgc ttgccgttca aacagacact cctgagtgat gaagatgctg aacttgtgga
     1621 gatagtgaaa gaacggcttc gtaatcctaa gccagtacgt gtgacgctgg atgaactctg
     1681 atggcgtatt ttctggattt tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg
     1741 acggtacgtg aacagttgaa aaagaagctg gttgaagtac ttgagtcacc ccggattgaa
     1801 gcaaacaagc tccgtggtat gcctgattgt tacaagatta agctccggtc ttcaggctat
     1861 cgccttgtat accaggttat agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa
     1921 agagaacgct cggaagtata tagcgaggcg gtcaaacgca ttctctgaac caaagcatga
     1981 catctctgtt tcgcaccgaa ggtgacactt ctgctttgcg ttgacaggag aagcaggcta
     2041 tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga
     2101 cggcactggt aacgaggaaa gacctctgcg aggtacgaat ccgaaccggc cagacggagg
     2161 tcgctgtctt cacagcttac gaacctgagg agtaagagac ccggcggggg agaaatccct
     2221 cgccacctct gatgtggcag gcatcctcaa cgcacccgca cttaacccgc ttcggcgggt
     2281 ttttgttttt attttcaacg cgtttgaagt tctggacggt gccggaatag aatcaaaaat
     2341 acttaagtag cgcgcaggga taagagggat ggtcccttaa aggggagagc taattatccg
     2401 gaaggattct gatgatgaac atcgaagaac tgcgtaaaat tttttgtgaa gatggcctct
     2461 atgctgtgtg cgttgaaaat ggaaatcttg ttagtcatta ccgcattatg tgtttgcgaa
     2521 agaatggggc tgcgttaatt aattttgtgg atgctcgggt cacggacgga tttatcttgc
     2581 gcgaaggtga gtttgtcact tcattacagg cattgaaaga gatcggaata aaagctggct
     2641 tttctgcttt ttcaggagaa taaactcatc tacaatcttg cgcggggctg aactcccgct
     2701 gagtaacacc gtgccaccgg agaaaaccga tggcacgcaa cgcaaaatat tacaattctg
     2761 ataattcgcc cgttcttgcc tgcacgcacg ggcggtattc tcacgcattc aagtctgaat
     2821 ggttccagca ccctccatgc actgcagaac aggccgaatg gctgattcat tcttaccgca
     2881 ggcgcgggtt cgaggttaag aaagctctca gtctcgacta tcggcactgg ataatctctg
     2941 tcaggctgcc ttattccgaa cgcccaccac gtgcgtcccg cactttccag caacggatct
     3001 ggaggtaacg tgcgggtatt acttagacct gttctggtgc ctgagcttgg gctggtggtc
     3061 cttaagccgg gccgtgaatc catacagata tttcataatc ctcgagtgct ggtggaaccg
     3121 gaaccaaaaa gcatgcgtaa tctgccatcc ggagtcgttc ctgccgttcg ccagccgctg
     3181 gcggaagaca aaacattgct gccgtttttt agtaacgaac gggtgattcg tgctgctggc
     3241 ggcgttggcg cattgtccga ctggctatta cgtcatgtta catcctgcca gtggcctaat
     3301 ggcgattacc atcacactga aacagtcatt caccgttatg gtaccggcgc aatggtgttg
     3361 tgctggcact gcgacaacca actgcgtgac cagacatcgg aatcactgga gctgcttgct
     3421 caacaaaatc tgacagcatg ggtgattgac gtcatccgtc acgcaataag cggtacgcag
     3481 gagcgggaat tatctttggc tgaattatcc tggtgggcgg tctgcaatca ggtggtggat
     3541 gcactacctg aggctgtatc gcgtcgttcg ctgggattac cagcggaaaa aatctgctcg
     3601 gtgtaccgcg aaagcgacat cgtaccggga gagcagaccg ccaccagcat attgaaacaa
     3661 cgcacaaaaa atcttgcacc gttgccttac gcccaccagc aacaaaaatc accacaggaa
     3721 aagacggtgg taagcatcac cgttgatcca gagtctccgg aatctttcat gaagctgcct
     3781 aaacgtcgcc gctgggttaa ggagaaatac acacgttggg ttaagacaca gccgtgtgct
     3841 tgctgcggta tgccagccga cgatccgcat catctgattg gtcacgggca gggcggaatg
     3901 ggaacaaaag cacatgatct ctttgtgttg cctttgtgca gaaagcatca caacgagctg
     3961 catacggata cagtggcatt tgaagataag tatggctccc aactggagct gatatttcgt
     4021 tttatcgatc gcgcgctggc aattggcgta ctggcgtaag tggagaacga gcatgaacct
     4081 tgaagcctta ccaaaatatt actccccaaa atctccaaaa ttgagcgatg acgctccagc
     4141 gacaggcacc ggttgtttaa caattacgga tgtaatggca gcgcagggga tggtgcagtc
     4201 gaaagcacca cttgggttgg ccttatttct ggcaaaagtt ggtgttcagg accctcagtt
     4261 tgcgattgaa ggcctgctaa attacgcgat ggcactggat aacccgacat tgaacaaatt
     4321 gagtgaagaa atccggttac agattattcc ttacctcgtg agttttgcct ttgctgatta
     4381 ctccaggtct gcggcaagta aggctcgctg tgagcattgt tcaggtacgg gattttataa
     4441 tgtattgcgc gaagtggtga aacactacag acgcggggaa tctgtaatca aggaagaatg
     4501 ggtgaaggaa ctatgtcagc attgccatgg taagggcgaa gccagcacag cgtgcagagg
     4561 gtgtaagggt aaagggattg ttctggatga aaaaagaacc cggtttcatg gcgtaccggt
     4621 atataagatt tgtgggcgtt gtaatggaaa ccggtttagt cgtttaccga ccacgctggc
     4681 acgacgtcat gtccagaagc tggtaccaga cctgaccgat tatcagtggt ataaggggta
     4741 tgcggacgtc attggtaaac tggtaacaaa gtgctggcag gaagaagcat acgcggaagc
     4801 gcaattgagg aaggtgacga gataaatgat ttttgctgaa gatggcgaca tgatgtttgc
     4861 atttttcaaa aaatatggat aaaatttttt caacgatggg ctttgtatac ccgacgttaa
     4921 gaaaaagtag aaaacccgct gatgagcggg ttttgtgctt taaatggggc aatggtaatg
     4981 ttgaatctca tcccgggact catgtctgtt aacttattat ttagctggtg acttggttat
     5041 ttgcctgatg tttaaaatgt tttcttccag tacaatgtcc ctaaacacaa tgagtctgct
     5101 tattatatta ttagcagagc tattacggcc aaagtacagc ataagctttt aaagccaatc
     5161 aaccagtcat caagacagac ggggttattc ataaaaactc tccatgtgtg atccgatggg
     5221 gcctgaaatt aaagctttaa tatagctcat gaaaggtaaa cattggcagc tgaagggcca
     5281 cgcagaccat ttatccggca aaattccacg cgtaatccgg tggtaatttc ttctgcatcg
     5341 cggagattga gcgctgaaac atgaagctgg acatcgatac gaccatcgga tggggtgata
     5401 agacccttgc cgcttttgcc gtcaaaggtt ttgacaattc ctgtcatttt acgggacaaa
     5461 aaaattcctt aatactgata acttggcgca ctatacacac gttcctgaag aaagctatag
     5521 ttttttgatg gggttgaaga tggctggatg tctaaaataa acattgcttc atatgttcaa
     5581 ctatgcgtta atgattgcgt cggtttgaag aacagacgat atacgaagta gtttactaaa
     5641 gcagttctca tttcaggtgt tattcactta ttccttcttt gagtctctcc aattaagtac
     5701 gaagtcgttt ctgttatgca aaccatttat gccgaaaggc tcaagttaag gaatgtagaa
     5761 tgtcaaataa aatgactggt ttagtaaaat ggtttaacgc tgataaaggt ttcggcttta
     5821 tttctcctgt tgatggtagt aaagatgtgt ttgtgcattt ttctgcgatt cagaatgata
     5881 attatcgaac cttatttgaa ggtcaaaagg ttaccttctc tatagagagt ggtgctaaag
     5941 gtcctgcagc agcaaatgtc atcattactg attaaaattc atcgctcgtc tgtatacgat
     6001 aacgaagaag gctgatgcct gagtagagat acggacagag tagtgaatat tggatctctt
     6061 taataaaaag taaggaggtc caatacatga aacaatggct agcatatttg gcaaaatctt
     6121 aatcaggaaa agtatgctaa ccattgtggt gaagtgcagg tttgctgcat gaatagtttt
     6181 acagcagaag ctaactgctg gcatggcaaa acaaagtgcg taagtggatg actcccacaa
     6241 aaagcaccac aatctcaaac ccgctcaggc gggtttttta ttatctgctt taaatatatt
     6301 attaaaatat aaaaaatact tgttactaat aaaatcaatc aggctacagc tttaagattt
     6361 gtctggaata ctttgttgca atgagggcag atcaaaaggg cacctttttg tactcttgaa
     6421 aaactgtgtt ctgactcttg ggtgcagttt gggcaggaac atttaacgag ataattacgg
     6481 cgtgattttg agtttttacg ttctgacata ggctttt
//
LOCUS       DBBGLQ010000176         6486 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.176, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000176 DBBGLQ010000000
VERSION     DBBGLQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.176"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            65..1252
                     /locus_tag="ACIYSB_004996"
     CDS             65..1252
                     /locus_tag="ACIYSB_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGC5431850.1"
                     /translation="MLMKYISHRAIICTLTLLIIIITVLFTFLRSSDVPEYITAPVRK
                     GDIENSVLATGRIDAIERVNVGAQVSGQLKSLKVKQGDHVTKGQLIAEIDDLPQCNDL
                     RNAEAALNEVKAELQSKQALLKQAELRFKRQLRMLRENASSHEDFESAEAMLATTRAE
                     LLSLNAKLVQAQIEVDKKKLALEYTRVVAPMDGIVIAIVTQQGQTVNSNQSAPTIIKL
                     ARLDVMTIKAQISEADITRISVGQKARFSIFSEPDKHYSATLRAVELAPESVMKDDSL
                     ASNTSASGSGTSNASVYYNALFDVPNPENRLRIAMTAQVTLITDEAQNTLLVPIQAVH
                     RNEGKKQQVLVLAADGRLEPRNVKTGITNSVDIQILEGLNVGENVVLSLPDKKEPEER
                     IML"
     gene            1249..3189
                     /locus_tag="ACIYSB_004997"
     CDS             1249..3189
                     /locus_tag="ACIYSB_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HGC5431851.1"
                     /translation="MKKLIELKGVSRTYGNGDQTRTVLKNVDLTIVAGEMVAIIGASG
                     SGKSTLMNIMGCLDVPNRGDYYIDGQNAACLSPDELARVRREHIGFIFQRYHLIPDLS
                     ALGNVEIPAIYANSERDSRRQRATALLGRLGLEGREHHKPCELSGGQQQRVSIARALI
                     NGGKIILADEPTGALDSQSGQEVLAILNELNRRGHTVVMVTHDMKVARHAKRIIELCD
                     GEIIADSGGCVSATETLPKTNRIRQSYWKTLLDRTRESMQMALKAMKTHRLRTTLTMI
                     GIVFGIASVVTVVALGEGARQETLEEIKSLGTNVVSIYPGQDLFDDSIESIRTLVPAD
                     ANALAKQGFIDSVSPEVSASDNIRFLGKSAIASINGVGREHFRVKGIELLQGTTFRDD
                     RNALQEVIIDENTRKAIFDNTGLQALGQIVFLGSVPARVVGIAKSNNRSDASNRITVW
                     MPYSTVMYRIVGKPVLTGISVRLKDNVDNEAAISAISQLLTRRHGIKDFQLYNFEQIR
                     KSIEHTSMTFSILILMVACISLMIGSIGVMNIMLISVTERTHEIGVRMAVGARRSDIM
                     QQFIIEAVLVCLIGGALGIALSYITGALFNALADGIFAAIYSWQAAVAAFFCSTLIGI
                     IFGYLPARKAARMDPVISLASE"
     gene            3256..4119
                     /locus_tag="ACIYSB_004998"
     CDS             3256..4119
                     /locus_tag="ACIYSB_004998"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HGC5431852.1"
                     /translation="MLKSDYRAPEVNYPINWTKGDVDGNTSPFDWEEFNDPNLDNWLH
                     LVMTSNNDIAIAALRIHRAQLDAERTGITNTPTLKAALSMDGKKQLNNSSGWAKSGSA
                     SLGTSYELDLWGKIARQRDVAEWAVHASEEDFRSARLMLLSEASNNYWRIGFVNQQIT
                     TLQQSIDYAKETLRLAEVRYRAGNISSLDVIDAQQNLLTQENQLTGLQREHSQLLNQQ
                     AVLLGTVPGCQIVEPTTLPKGSLPKVNANIPASILMRRPDISAKEWQLREALAALLQI
                     VGGDKLIIPFC"
     gene            4131..4835
                     /locus_tag="ACIYSB_004999"
     CDS             4131..4835
                     /locus_tag="ACIYSB_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HGC5431853.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            5003..6439
                     /locus_tag="ACIYSB_005000"
     CDS             5003..6439
                     /locus_tag="ACIYSB_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGC5431854.1"
                     /translation="MSTLDVQKLKDDAVEWALTHGVAFKESSYSAVHTPFTLTPTPIS
                     RKSYQYLKNATGILSKLIYSVSEDHDFLYSAIYPIKAGNAFFSALLNMHQQIHSSSRH
                     APRLPLLIMRSDFMDDNRDGFRLIEFNGIAAGMGPFGQRIHDLHHYLQRQCPAVFSDL
                     SNQATGALVDNQAIEGLSAAICQATCRIKQEFGDPGPARFLMIVQENENNVFDQHLLE
                     LALQQKGIETHRRTFRELHNQLSTGADNRLILEGVGPLDTVYLRAGYQYSDYEAFNID
                     EQECCQALMATRVFIEQHRVAVNATVGQQLATSKRVQMLLSQLGYDEFVAFGLTLQEA
                     KIAKTILGEMLPISPDSINLAKESPSETWVLKNQGEGGGHCLFGADILTKLTELTPQQ
                     YQSWSLMRRLHPQPRAMPTLIVRKGELHKVNDLISELGMFSVQTDNNPSSAEHSFAGY
                     LIRSKSAESTEGGVHSGQGVLDSLVYSD"
BASE COUNT         1783 a         1508 c         1625 g         1570 t
ORIGIN      
        1 agatgctggg cattaactaa gaaaacccag aataccactt tcattgttat ggaaaacatg
       61 gacaatgtta atgaaatata tttctcaccg cgccatcatt tgtaccttga cactgttgat
      121 cattattatc accgttttgt tcacctttct gagaagctct gatgtgccag aatatattac
      181 tgccccggtg cgcaaaggcg atatagaaaa ttctgttctg gcgacaggcc gtattgatgc
      241 aattgaacgg gtaaacgtag gggcacaggt atcagggcaa ttaaaatcac tgaaagtgaa
      301 acagggcgac catgtgacta aagggcagtt gattgccgaa attgacgacc tacctcagtg
      361 taacgatttg cgtaatgccg aagctgcact caacgaagtg aaagcagaac ttcaatccaa
      421 gcaggcgctc ctgaaacagg ctgaactgcg ttttaaacgc cagctccgga tgctaagaga
      481 aaatgccagc tcacacgaag attttgagtc tgcggaggca atgcttgcga ccactcgcgc
      541 tgaattactg tctctgaatg ccaaactggt ccaggcacag atagaagtag ataagaaaaa
      601 gcttgccctt gaatatacgc gagtcgtagc gccaatggat ggaattgtta ttgccattgt
      661 cactcagcaa gggcaaaccg ttaactcaaa ccagagtgcc ccaacaatta ttaaactcgc
      721 tcgtctggat gtcatgacca ttaaagcaca gatctccgag gctgatataa cccgtatttc
      781 tgtggggcaa aaagcccgct tctctatctt ctccgaaccg gacaagcact acagcgcaac
      841 actgcgtgcc gttgaactgg caccagaatc ggtaatgaaa gatgactctc tcgccagcaa
      901 cacttcagca tcaggctccg gaacttcaaa tgcgtccgtt tattacaatg ctctattcga
      961 tgtgcctaac ccagaaaatc gacttcgtat tgccatgact gctcaggtta ccctgattac
     1021 tgatgaagcc caaaatacac tgctggtacc cattcaggca gtgcacagaa atgaggggaa
     1081 gaagcaacag gtcctggtgc tggcagcaga tggcaggctg gaaccccgga atgtgaaaac
     1141 tggtatcacg aacagcgtgg atattcagat cctcgaaggt ttaaatgtcg gagaaaatgt
     1201 cgtgctgtcg ctgccggata agaaagagcc cgaagaaagg attatgctgt gaaaaaactt
     1261 attgagttaa aaggggttag ccgaacttat ggcaacggag atcaaacgcg gaccgtcctg
     1321 aaaaatgtcg atttgacaat cgttgctgga gaaatggtgg caatcattgg agcctctggc
     1381 tcaggcaaat caaccttaat gaacataatg ggttgccttg acgtacccaa tagaggtgat
     1441 tactacatcg acggacaaaa tgccgcctgt ctttcacccg atgagctggc tagagtgcgc
     1501 cgggaacaca ttggctttat attccagcgc taccatttga tacctgatct tagcgcactt
     1561 ggtaacgtgg agatcccggc catttacgcc aacagcgaac gcgacagccg tcggcaacgt
     1621 gcaacggcgt tgctgggcag actgggactg gaaggacgtg agcatcataa accttgcgaa
     1681 ctttcaggcg gtcagcagca acgcgtcagt attgcccgag cactgatcaa cggtggaaag
     1741 ataattttgg cggatgagcc aactggtgct ctggattcgc aatccgggca agaagtgtta
     1801 gccattctga atgaacttaa tcgacgtggt catactgtag taatggtgac ccacgatatg
     1861 aaggtcgccc ggcacgcaaa gcgaatcatt gagctgtgtg atggcgaaat catcgctgat
     1921 agtggaggtt gcgtatctgc gacggaaaca ctaccaaaaa ccaacagaat ccggcaaagc
     1981 tattggaaaa cactgctcga tcgaacgcgt gaatcgatgc aaatggcgct aaaagcgatg
     2041 aagacgcatc gcttacgcac cacgcttacc atgatcggga ttgtttttgg tatcgcctcc
     2101 gttgtcactg ttgtggcatt gggtgaagga gccaggcaag aaacactgga agagattaaa
     2161 agtctgggga caaacgtggt cagcatttat cccgggcagg atttgttcga tgacagcatt
     2221 gagagtatta gaacattggt acctgctgac gcaaatgcac tggcgaagca agggtttatc
     2281 gatagcgtca gcccggaagt tagcgcttca gacaacattc gttttctggg taaatctgcg
     2341 atagcgtcca ttaacggcgt tgggcgggaa catttccggg taaaaggtat tgagttgttg
     2401 caagggacta cttttagaga cgatcgcaac gctctacagg aagttattat cgatgagaat
     2461 actcgtaagg ccattttcga caacacaggg ttacaggctc tggggcaaat agtctttctt
     2521 ggttctgtac cagcacgagt ggttggcatc gctaaaagta acaaccgtag tgatgcatca
     2581 aatcgcatta ctgtgtggat gccttacagc acggtgatgt accgtattgt gggtaaacca
     2641 gttctgaccg gtattagcgt caggctaaaa gacaatgtgg ataacgaggc tgcaatcagc
     2701 gctatctccc aactattaac ccggcgtcac ggcataaaag acttccagct ttataacttt
     2761 gaacaaataa gaaagtctat cgaacacacc tcaatgacct tcagtatttt aattcttatg
     2821 gtcgcatgta tctcactaat gatcggcagt atcggggtga tgaacatcat gttgatttct
     2881 gtaacggaac gaactcatga aattggcgta cgaatggcgg ttggcgcgag acgcagcgac
     2941 attatgcaac agtttattat tgaggccgta ttggtttgcc tgattggtgg cgcactcggt
     3001 attgcactgt cgtatatcac aggtgcgctc tttaacgctc tggcggacgg gatattcgca
     3061 gctatatatt cgtggcaggc tgctgttgct gcattttttt gctcaacctt aatcggcata
     3121 atcttcgggt atctcccagc ccgcaaggca gcaaggatgg acccggttat ttcactggcc
     3181 agcgaataat atatgaaaat tttatcatcg ttgactttat gccttatcac cgcactctgt
     3241 tcaggttgca caaacgtgct gaaaagtgac tatcgcgctc ccgaagtgaa ctatcccatc
     3301 aactggacca agggcgatgt ggacggaaat acatccccgt ttgactggga agaatttaac
     3361 gatcctaatc tcgataactg gcttcacctg gtgatgacaa gtaataacga catagctata
     3421 gctgcactgc ggatacatcg ggcgcagctg gatgcggaaa gaacaggtat caccaatact
     3481 cctacactaa aggcggcact gagtatggac ggaaaaaaac agctgaataa ctcgtccggc
     3541 tgggcaaaaa gcggttctgc cagccttggc accagctatg agctggatct ctggggaaaa
     3601 attgcccgtc agcgtgatgt tgctgagtgg gcagtccatg ccagtgaaga agattttcgc
     3661 tccgcgcgcc tgatgctgct ttcagaggcc agcaataatt actggcgtat cggttttgta
     3721 aatcaacaaa ttaccaccct ccagcagagt attgactatg caaaagagac gctgcgtctg
     3781 gccgaggttc gctatcgtgc aggaaatatc tcgtcgctgg atgtgattga cgcgcagcaa
     3841 aacctgctca cgcaagagaa ccagcttaca gggctacaac gagaacattc acagctactc
     3901 aatcagcagg ccgttttgct cggcaccgtg cccggttgcc agatagtcga gcccacaacg
     3961 ctgccaaaag gaagcctgcc taaggtcaat gcgaatattc ctgccagcat actgatgcgc
     4021 cggcctgaca taagtgctaa agagtggcag ttgcgtgagg cgctagcggc actgttgcaa
     4081 atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac atgaacccat
     4141 tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat
     4201 acggcatcag ttaccgtgag ctgcaggaga tgctggctga acgcggagtg aatgtcgatc
     4261 actccacgat ttaccgctgg gttcagcgtt atgcgcctga aatggaaaaa cggctgcgct
     4321 ggtactggcg taacccttcc gatctttgcc cgtggcacat ggatgaaacc tacgtgaagg
     4381 tcaatggccg ctgggcgtat ctgtaccggg ccgtcgacag ccggggccgc actgtcgatt
     4441 tttatctctc ctcccgtcgt aacagcaaag ctgcataccg gtttctgggt aaaatcctca
     4501 acaacgtgaa gaagtggcag atcccacgat tcatcaacac ggataaagcg cccgcctatg
     4561 gtcgcgcgct tgctctgctc aaacgcgaag gccggtgccc gtctgacgtt gaacaccgac
     4621 agattaagta ccggaacaac gtgattgaat gcgatcatgg caaactgaaa cggataatca
     4681 acgccacgct gggatttaaa tccatgaaga cggcttacgc caccatcaaa ggtattgagg
     4741 tgatgcgtgc actacgcaaa ggccaggcct cagcatttta ttatggtgat cccctgggcg
     4801 aaatgcgcct ggtaagcaga gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg
     4861 cctcatcgct aactttgcaa cagtgcctct tttttcataa ttcaatgcta caagaactat
     4921 tgaagtaatc aatcaaattt tataggctga tagccaagta acaaccacca atgatatgcc
     4981 aggtaaaatc aggggacgta aaatgagtac tcttgatgtt cagaagctca aggatgatgc
     5041 cgtcgaatgg gccttaactc atggtgttgc tttcaaggaa tcgtcgtatt ccgcagtgca
     5101 taccccgttt accctgacac caacacctat cagccgtaaa tcctatcagt acttaaagaa
     5161 tgcaacagga atactcagca aattaatcta ttcagtttct gaggatcatg attttttgta
     5221 ctctgcgatt tatcccatca aggccgggaa tgccttcttt tcagccctcc tgaacatgca
     5281 tcaacaaatc cattcatcat caaggcatgc tcctcgatta ccattactca ttatgcgtag
     5341 cgactttatg gatgataatc gtgatgggtt caggcttata gagttcaatg gtatagctgc
     5401 aggaatgggg ccgtttggtc agcggataca tgatctgcat cactatctac aacggcaatg
     5461 tcccgctgtc ttttctgatt tatcgaatca ggcaacaggg gcgcttgtcg acaatcaagc
     5521 gatcgaaggg ttatcagcag cgatttgtca ggcaacttgc agaattaagc aggagttcgg
     5581 agaccccggc cctgccaggt tcctgatgat tgtgcaggag aatgaaaata atgtgtttga
     5641 tcaacacttg cttgagctcg ccctccagca aaaaggaatc gaaacgcatc gcaggacttt
     5701 tcgtgaactg cataatcaat tgagtacagg cgccgacaac aggctcattc tcgagggtgt
     5761 aggcccactt gataccgtct acttacgtgc cggatatcag tattctgatt atgaagcatt
     5821 caatatcgat gagcaggagt gttgtcaggc gctgatggcg acccgagtat tcattgagca
     5881 gcaccgggtt gcagtgaacg ccacagttgg ccagcaactg gcgaccagca agcgagtaca
     5941 aatgctgctc tcgcaattgg gttacgacga gtttgtcgca tttggattaa cactgcagga
     6001 agctaaaata gcaaaaacga tactgggtga gatgttaccc atcagcccgg atagtatcaa
     6061 ccttgcaaaa gagtctcctt cggaaacctg ggttctcaaa aatcagggtg aaggcggggg
     6121 gcattgtctg tttggtgcgg atatcctgac aaaactgacg gaactcactc cacaacaata
     6181 tcagtcctgg tcgctgatgc gccgtttaca ccctcagccc cgagctatgc ccaccttgat
     6241 tgtgcgtaaa ggggagttgc ataaggtgaa tgatttgata agcgaacttg gaatgttctc
     6301 cgtgcagaca gataacaatc cctccagtgc tgagcacagt tttgctggat atctaattcg
     6361 tagcaaatca gctgaatcga cagagggagg ggtccatagt ggacaaggtg ttttagactc
     6421 gctggtatac agcgattagt ccttttcaag ggggctgaaa tctggttata tgaaagcatg
     6481 cggtaa
//
LOCUS       DBBGLQ010000177         6479 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.177, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000177 DBBGLQ010000000
VERSION     DBBGLQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.177"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(40..279)
                     /locus_tag="ACIYSB_005001"
     CDS             complement(40..279)
                     /locus_tag="ACIYSB_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431855.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDMDIHKKNRYASYDLRRKIEIQHEEFHV"
     gene            complement(385..660)
                     /locus_tag="ACIYSB_005002"
     CDS             complement(385..660)
                     /locus_tag="ACIYSB_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGC5431856.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(660..944)
                     /locus_tag="ACIYSB_005003"
     CDS             complement(660..944)
                     /locus_tag="ACIYSB_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGC5431857.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVKSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            1445..2035
                     /locus_tag="ACIYSB_005004"
     CDS             1445..2035
                     /locus_tag="ACIYSB_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGC5431858.1"
                     /translation="MSRIFAYCRVSTLEQTTENQRREIESAGFNVRPQRIIEEQISGS
                     VAASERPGFNRLLDRLESGDVLIVTKLDRLGRNAMDIRKTVEQLTESGIRVHCLALGG
                     VDLTSPAGKMTMQVISAVAEFERDLLLERTYSGIARARDAGKRFGRPPVLNEEQKQKV
                     LERIKSGASISAIAREFRTTRQTILRVRAKQQTVTV"
     gene            2082..2240
                     /locus_tag="ACIYSB_005005"
                     /pseudo
     CDS             2082..2240
                     /locus_tag="ACIYSB_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2390..3388
                     /locus_tag="ACIYSB_005006"
     CDS             2390..3388
                     /locus_tag="ACIYSB_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGC5431859.1"
                     /translation="MKKVLCALGLMFTAVSSALATTYPLTIENCGYQETFTRPPERVV
                     ALGQNTVEILLLLGLQKQVVASAFWPTNVLPQLAEQNAKIKTLTVEIPSLESVLAQNP
                     DFVPAQLPLLLGPESKVARREDLATVGVNSYVSPGVCATKKATGDMYGSRQKLWDMTW
                     LYQEISDFSRIFNVEDRGQALIADFKKREADLRQEFGKSKKDLSFVFWFSSASPSADA
                     YVGGKNSASGFIASVLGGHNAITSETEWPTVSWESIIAANPDVIVVASLDRNRWALDK
                     AEEKIKFLKSDPAVSQLEAVKKGHIVVMDGQAMNPTIRTLYGAEQVGEQLRKMGLN"
     gene            3388..4425
                     /locus_tag="ACIYSB_005007"
     CDS             3388..4425
                     /locus_tag="ACIYSB_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGC5431860.1"
                     /translation="MSVGAIETCRSILLTGWCVLAAIVLALVIAVGVSVGELAIPLQN
                     VFYAISNRTGLTAEPLNRIYESVIWDFRLSRALVAACCGAGLAICGVVLQSLLKNALA
                     EPYVLGVSAGASTGAVSIVVLGLGSGAISLSAGAFAGAFAAFAFVALLTNGARGGNER
                     TILAGVAASQLFNAITAYTISTTASAQQARDVMFWLLGSFSGVRWPEFQLVIVVVLAG
                     LAVCLWYARALDAFTFGDDAAASLGIAVPRVRLILFTTAALITATIVSMAGSIGFVGL
                     VVPHVMRFFFGPLHRTLLIASALAGAILMVLADIASRLLIAPQSLPVGVVTALVGVPF
                     FAVIIYRSRNK"
     gene            4425..5186
                     /locus_tag="ACIYSB_005008"
     CDS             4425..5186
                     /locus_tag="ACIYSB_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5431861.1"
                     /translation="MSICAENITWKAGKKVIVNNVSLRVPRGETVGLLGPNGCGKSSL
                     LRVLAGLRRPDAGRVTLDGQDIARMAKKQLARRVACVEQHGMTEANMRVRDVVRLGRI
                     PHHSPFSNWSAQDDEAIAAALQRVAMLEKSEQGWLSLSGGERQRVHIARALAQSPSEI
                     LLDEPTNHLDIHHQMQLMQLISELPVTSIVAIHDLNHAAMFCDSLIVMQQGQILASGT
                     PEEILSEALLWDVFRVKTKIEISPYHGKKHIHFIV"
     gene            5243..6430
                     /locus_tag="ACIYSB_005009"
     CDS             5243..6430
                     /locus_tag="ACIYSB_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HGC5431862.1"
                     /translation="MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLIGLILGLRTF
                     SQQGMFILGGTLADRYGAKAIILAGCVVRVAGFLLLACGASLWAIILGACLTGVGGAL
                     FSPSIEALLARAGTHSQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIA
                     LAGAGIFLLALAVLFFGLPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSYNQ
                     LYLALPVEIQRSGGREQDLAPLFMLASLLIITLQLPLARFARRMGAVRILPVGFLLLS
                     ASFASVALFAAAPPAESWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAH
                     YGALATAGGCAVLAGNLLLGHLLDQALIPSPQAVYPWLLLALFPLCSAVALRAICRPL
                     AAT"
BASE COUNT         1334 a         1809 c         1822 g         1514 t
ORIGIN      
        1 taagacgcgg acagtggtat cgcttagttt ttcaatttcc tatacgtgaa actcttcatg
       61 ctgtatttcg attttccttc taagatcata actcgcatac ctgttctttt tatgtatatc
      121 catatctatt tttgtgacga gttcaaacca caatgttcgc actccccatg cagcgttatg
      181 aaacatttcc gcaacggcta tagagtctgt attaactgct tcggtcatgt agtcgctaat
      241 acgttcagcg gtaattttcg ttatttcatc gtaattcatt ttatctccta caagattatt
      301 taatggaatg aaaaatatct ggcctgataa taatgttcca gaattaatat ctgcaggcta
      361 tgttaagttg tccccacaca caatctacct gttttctcgt gtgtgccaca ggcgcaatac
      421 ataaatagtc tggccattaa gttcgtaacg gatttcgtac tcgccagcaa aaatccgtct
      481 gacctccctg ggttcaaact gaaacaactg ttctcccata cgtggatgag ttaacagaat
      541 gaccggtgcc tgtgtcaggg actgcaccgt tctggcggcc gcaggtttac tggccagcac
      601 cagaaaatca tataaccgcg ccaaatcaga aagcgcctta ctggtccact tcagttccat
      661 cagcgtggca ccggtaacgg attgtcagta ctgaggctgt ccgcccaggc ctgtacagcc
      721 tgatggtcga taacctgtcc ggatttcaca tcgtccaggg cttccagcgt caggcgatta
      781 cgctcctcct cctgggcaag ccatgcagaa agcgcctgtt tgataaccca gccccgggag
      841 cgttccagtc tggcggccat ctggtcgact ttatcagcca ttggtaatgg cacatgcgcg
      901 gtaatgactt ttgttgcctg tgcggtattg tttttcattt gcatacccga ttagttgtta
      961 atcatattta atcatagtga ttactttcag acggaacaag gcaaataatg cgcgtcagcg
     1021 catgtaaggc ggcactccgt tgttacatat ggcacaggag taaaaccgcc gaagcccggc
     1081 gtaagccggt actgattgat agatttcacc ttacccatcc ccagccctgc taagccatac
     1141 ccgctttcag ccatgagaga gcttctgtgc gcggtcggag tggtcccgac gagggtttac
     1201 ccgaagtcgg gacgtatctc cgcgttagcg gggtcagttt ggaattcgga aaataaacca
     1261 tgttaagact attttttagc caaagggctt tcaggaatcg ccagaatggc gggacaacag
     1321 tggttttggt gtccgaaatc gacctgtttc ggacattgtt aaacagatat atccgaaagt
     1381 cagcgaaatt actttcggac atgcattata atatgtacac ccatttcgta cagaagatct
     1441 cactatgtct cgaatttttg cctattgtcg ggtttccact ctggaacaga cgaccgaaaa
     1501 tcaacgtcgg gaaatcgaaa gcgcgggttt taacgtcaga ccccaacgaa taatcgaaga
     1561 acaaattagt ggatcagtag cagccagtga acgtccaggt tttaaccggt tgcttgatcg
     1621 tctggaaagt ggcgatgtat tgatagtcac aaaactggat cgcctcggtc gtaatgcaat
     1681 ggatatcagg aaaacagtgg agcaactgac cgaatctggc atcagggtgc attgcctggc
     1741 attaggtggt gttgatctga ccagtccggc aggaaaaatg acaatgcaag tgatttcagc
     1801 ggtcgctgaa tttgaacgag atcttttact tgaacgaact tattcaggga tagcaagagc
     1861 acgggatgca ggaaaacgtt ttggtcgccc accggtatta aatgaagaac aaaaacagaa
     1921 ggtactcgaa cgcattaagt ctggggcaag tataagtgct attgccaggg aattccgaac
     1981 cacacggcaa acaattctga gagttagagc gaaacagcaa actgttactg tatagaaaat
     2041 agtcttagcg tgggatattt tccgttttcc aagcggaccc caaggaagac tacatcgcgt
     2101 tcatgccgaa accgaatgta agaacggcat tgcataatct tgcagtggcg atcgaacatt
     2161 acaatgaaaa ccatccgcac agtgcgttgg gttatcgctc tccgcgagaa tatcgacgtc
     2221 agcgggtaac attaacttaa gatacaaccc ctgtctggaa ataagggggc aagaacacat
     2281 tgacactgta aatatggcat tgtataaaat cccgcgacga taatgagttt catttgcatt
     2341 actcccattt gctggtcatc cactcgcctg tttacaagga tagcgtttaa tgaagaaggt
     2401 cctttgcgcg ttaggcttga tgttcaccgc cgtcagttcc gcgctggcaa caacctaccc
     2461 cctgacgatt gaaaactgcg gctatcagga gaccttcacc cggccgccgg agcgcgtggt
     2521 cgcgctgggg caaaatacgg tcgaaattct gctgctgctc gggctgcaaa agcaggtggt
     2581 cgccagcgcc ttctggccga ccaacgtttt accgcagctg gctgagcaaa acgcgaagat
     2641 taaaaccttg acggtggaaa ttccgagcct ggaatcggtg ctggcgcaaa atcctgattt
     2701 cgttcccgcc cagttgccgc tgctgctggg gccggaaagc aaagtcgcca ggcgtgaaga
     2761 tctcgccacc gtcggcgtga acagctacgt ctcaccgggg gtgtgcgcca ccaaaaaggc
     2821 caccggcgat atgtacggca gccggcaaaa gctgtgggat atgacctggc tgtatcagga
     2881 aattagcgat ttttcccgca ttttcaacgt cgaagatcgt ggtcaggcgc tgattgccga
     2941 tttcaaaaaa cgtgaagccg acctgcgcca ggagtttggt aaaagcaaaa aggatctctc
     3001 cttcgtcttc tggttctcca gcgcctcacc ttcggccgat gcctatgtcg gcggcaaaaa
     3061 cagcgcctcc ggatttatcg ccagcgtgct gggcggacat aatgcgatta cctccgaaac
     3121 cgagtggccc acggtgagct gggagagcat tattgccgcc aacccggatg tgatcgtggt
     3181 cgccagcctg gatcgtaacc gctgggcgct ggataaagcc gaggagaaga tcaaattcct
     3241 caaaagcgat cccgccgtca gccagctgga ggcggtgaaa aaaggccata tcgtggtcat
     3301 ggacggtcag gcgatgaatc cgaccattcg cacgctttac ggcgcggagc aggtgggcga
     3361 gcagctgaga aaaatggggc tgaactgatg agcgtcggcg cgattgagac gtgccgcagc
     3421 atactgctca ccggctggtg tgtactggca gcgatcgtgc tggcgctggt gatcgctgtg
     3481 ggcgtcagcg ttggcgagct ggccatcccg ctgcagaatg tcttttatgc tatcagcaac
     3541 agaaccggct taaccgctga gccgctcaac cgtatctatg agagcgttat ctgggatttc
     3601 cgcctcagcc gcgcgctggt cgccgcctgc tgcggcgccg gcctcgctat ctgcggtgtc
     3661 gtgctgcaaa gcttgctgaa gaatgccctc gccgaaccct acgtcctcgg cgtctccgct
     3721 ggggcctcca ccggcgcggt gtcgatcgtg gtgctgggcc ttggctctgg cgcaatttcc
     3781 ctctcggcag gcgcctttgc cggggccttc gccgccttcg cctttgtcgc cttgctgact
     3841 aacggcgcgc gcggcggcaa tgagcgtacg attcttgcgg gcgtcgccgc ctcgcagtta
     3901 tttaacgcca tcacggccta taccatcagc accaccgcca gcgcgcagca ggcgcgcgat
     3961 gtgatgttct ggctgctggg cagcttcagc ggcgtccgct ggcccgaatt ccagttggtc
     4021 atcgtggtgg tgctcgcggg tctggcggtc tgcctgtggt atgcccgggc gctggacgcg
     4081 ttcaccttcg gcgacgatgc cgccgcctcg ctggggattg ctgtgccgcg cgtgcgcctg
     4141 atcctcttca ccactgcggc gctgatcacc gccaccatcg tcagcatggc cggctcaatc
     4201 ggctttgttg gcctggtggt gccgcacgtg atgcgtttct tctttggccc gctgcaccga
     4261 acgttgctga tcgccagcgc gctggcaggg gcgatattga tggtactggc ggacattgcg
     4321 tcgcgcctgc tgattgcccc gcaaagtctg cccgttgggg tagtaaccgc cctggttggg
     4381 gtgcctttct ttgccgtgat tatctaccgc tcaaggaata agtgatgagt atttgcgctg
     4441 aaaatatcac ctggaaggca ggcaaaaagg tcatcgtcaa taatgtctcg ctgcgggtgc
     4501 cgcgcggcga aacggtcgga ctgctggggc ccaacggctg cggcaaatcc tcgctgctgc
     4561 gcgttctggc gggcctgcgc cgcccggatg caggtcgcgt caccctcgac ggccaggata
     4621 tcgcccggat ggcgaaaaag cagctcgccc gccgcgtggc ttgcgtcgag caacacggca
     4681 tgaccgaggc caatatgcgg gtgcgcgacg tcgtgcgcct gggacgcatt ccccaccact
     4741 ctccgttctc aaactggagc gctcaggatg acgaagcgat tgccgccgcg ctacagcggg
     4801 tagcgatgct ggagaaaagc gaacagggat ggttaagcct ctccggcggc gagcggcagc
     4861 gggtgcatat cgcccgcgcg ctggcgcaga gcccgagcga aatcctgctg gatgagccga
     4921 ccaaccatct ggatatacac catcagatgc agttaatgca gttgatcagc gagctgccgg
     4981 taaccagcat tgtggccatt cacgatctta accatgccgc gatgttctgc gattcgctga
     5041 tcgtgatgca gcaggggcag atcctcgcca gcgggacgcc ggaggagatt ttgtccgaag
     5101 cgctgctgtg ggacgttttc cgggtgaaaa ccaaaatcga gatctcccct taccacggca
     5161 aaaagcacat tcatttcatc gtttaggttg ggcggatatg cctcttttct ccctgcgccc
     5221 tgcggcaacc ctctggcctc cggtgttact gggaagccag ttcgttttta atattggctt
     5281 ttacgcggtc gtacctttcc tcgccctctt cctgcgcgac gacatgctgc tctcaggcgg
     5341 acttatcggc ctgatcctcg ggctgcgtac cttctcccaa caggggatgt ttatcctcgg
     5401 cgggacgctg gccgaccgct atggcgctaa ggcgatcatt ctcgccggct gcgtcgttcg
     5461 cgtcgccggt tttctcctgc tggcgtgcgg ggcgtcgctg tgggccatta tccttggcgc
     5521 ctgcctgacc ggcgtcggcg gcgccctgtt ctccccgtcg atagaggccc tgctggcgcg
     5581 ggccggtacc cacagccagg ccaacgggaa acgcagccgc gccgagtggt ttgcgctgtt
     5641 tgcggtatgc ggtgaactgg gagcggtgat cggcccggtg gccggaggcg tgctgagcgg
     5701 gatcggcttt cgacatatcg ccctcgccgg cgcgggaata tttcttctgg cgctggcggt
     5761 gctctttttc ggtctgcccg ccgacgggca cacgacaaca acgcgcagac gagtcccatg
     5821 gtggacgcct ttgcgccagc cgcgctttgt cgcgtttatt ctcgcctaca gctcatggct
     5881 gttgagctac aaccaactct acctcgctct gccggtggag atccaacgtt ccggcggccg
     5941 cgagcaggat ctggcgccgc tctttatgct ggcctcgctg ttgattatca ccctccagct
     6001 ccctctcgcc cgcttcgccc ggcgaatggg cgccgtgcgc atcctgccgg tgggctttct
     6061 gctgctgtcg gcttcctttg ccagcgtggc gttattcgcc gccgccccac ccgccgaaag
     6121 ttggctgcgg ttaatgccgg cggctggttt tgtcactctg ctcaccctcg gccagatgct
     6181 gttagtgccc gcagccaagg atctgatccc gctgttcgcc gaggagtcga cgctgggcgc
     6241 ccactacgga gcgctcgcca ccgcaggagg gtgcgcggtg ttagccggga atttactgct
     6301 cggtcattta cttgaccagg cgctcattcc gtcgccgcag gcggtttatc cctggcttct
     6361 gctggcgctg tttccgctgt gcagcgccgt cgccctgcgg gcgatctgtc gcccgctggc
     6421 ggcaacatga cgcagagcgg gtaacagccc gctctggaag tgaacagctg gtatccata
//
LOCUS       DBBGLQ010000178         6477 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.178, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000178 DBBGLQ010000000
VERSION     DBBGLQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.178"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..252)
                     /locus_tag="ACIYSB_005010"
     CDS             complement(<1..252)
                     /locus_tag="ACIYSB_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGC5431863.1"
                     /translation="MMKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRD"
     gene            complement(498..710)
                     /locus_tag="ACIYSB_005011"
     CDS             complement(498..710)
                     /locus_tag="ACIYSB_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431864.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     IAPRTDWQQFARKKAEHCHRRCRGRV"
     gene            complement(846..1406)
                     /locus_tag="ACIYSB_005012"
     CDS             complement(846..1406)
                     /locus_tag="ACIYSB_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGC5431865.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            complement(1460..2206)
                     /gene="traX"
                     /locus_tag="ACIYSB_005013"
     CDS             complement(1460..2206)
                     /gene="traX"
                     /locus_tag="ACIYSB_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGC5431866.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSLWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLTVIPALNLATSDTAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            complement(2226..>6477)
                     /gene="traI"
                     /locus_tag="ACIYSB_005014"
     CDS             complement(2226..>6477)
                     /gene="traI"
                     /locus_tag="ACIYSB_005014"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HGC5431867.1"
                     /translation="ARTVGILPPENGVIERARAGIDETISREQLIPLDREKGLFTSGI
                     HVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGA
                     AGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFT
                     PGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTY
                     RWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRS
                     ELKTQGVLGHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRV
                     TAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGG
                     DRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFA
                     SVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETL
                     LETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFIDLGG
                     EINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGE
                     LMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVG
                     LGPTHRAVGEMRSAGVDAQTLASFLHDTQMQQRSGETPDFSNTLFLLDESSMVGNTDM
                     ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELRE
                     AVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAML
                     KGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGEL
                     GKEQVMVPVLNTANIRDGELRRLSTWETHRDALVLVDNAYHRIAGISKDDGLITLQDA
                     EGNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGD
                     SVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAG
                     FESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFS
                     TARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLN
                     PLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSG
                     VVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRE
                     TERRADEIVRKMAENKPDLPDGKTELAVREIAGQERDRAAITEREAALPESVLREPQR
                     EREAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD"
BASE COUNT         1245 a         2114 c         1635 g         1483 t
ORIGIN      
        1 atctcgctga aaatatgtga cggtaaccgt ttttcctgcg accagtgatt tcatcatgtc
       61 tgttgaccac cggccataat cctgtttctt ttccggtgca tcaatattta ccagcctgat
      121 ccgcaccgcc ttccgtgagt ccatgacctc gatggtatca ccgtccagta cacgaacaac
      181 ccgtccatga atatctgcag acaataccgg ccatgaaaag ataaataata ccagtggaat
      241 atatttcatc atgttattct ccatatcaga taaatggcac ctgtatttca ggcatcctga
      301 acattgcagc acacaatgaa taacacctta ttttgtgaaa taaatcacaa aatataaaat
      361 cacgacttaa tacggaaaaa actctgtgct actcagaact gtccggtcat actgcccgac
      421 cggatattaa aaaaagcaaa tctcataaac tcctttcagc cttctgactt ctggtgtaat
      481 gaaggctgct tttttcatta cacccttccc cggcagcgcc ggtggcagtg ttcagccttt
      541 ttccgggcaa actgctgcca gtctgtccgg ggagctatgc aggcagcccg tcgccatgct
      601 tctgccgctt ccatccagcg tccactgacc tccagaataa cggcccgccc gccggcacgc
      661 tggtaacgcc agacctgacc gttccgtgaa ctgtttctga atccattcat ttttctgctc
      721 ctccctttta gtcgtcagta ttattgtggt aaataattca gtggactggc ttcctttcgg
      781 gtacggaagt tatttccttt taagccttat tgtttcagcc tcctcactct ttcggggagg
      841 cctttttatt tctcatcaag cacggcctga agttctgcct ttatccggtt ctggcggcgg
      901 attttatcca gacgcgctgc cgcatacgct tcctcctcct gagaaatatg ctccgtcaca
      961 tacccttccg tgtcataccg gcaggcaccg gctttcatgg cacacagata gctttctgaa
     1021 cgggtgatgg ccttcagcgc cctgcgcagt tttttatgcg agagcgggat attccgctgc
     1081 gccacgtctt ccagtaacac gtcccggata ccgcaggcca gaagccgggg cgtgtcaccg
     1141 tcaaataatc ccggccacca gggcttcagg gtattcacgg cctcatccag tgagggcagg
     1201 ttcagataaa tggacagcgc ctgtctggcc tgcgcttttt ttgccgccag ctctgcttcc
     1261 cgggccgctt tctcggccag tttctgcttt ttcaccttcc attttggtgg cgtggtgaca
     1321 ttgatgatgg ttttccggct gcggacaggc gtctctcctt ctgttttccg cttcagtgtc
     1381 agtaccggtc gtttctgctc tgtcatgctg tgttctgcct ctgacgaatc atcctttacg
     1441 gtcaaagata ccacaccctt tacagtgcca gaacgcccag cacagccaga tggcaggcgt
     1501 agaaagtcgg gaaaaaatcc ccgggccaga aacggggtaa tgatttccct gcacaggaca
     1561 ccaggccaac ggtcagcacc gtcatcacaa ggccggccac cgccgctgta tcactggtgg
     1621 caaggttaag cgcaggaatc acagtcagca ggcatgcaac cagcaccaga cgttccgttc
     1681 tgtcttccgc ccggtacagc cggtgactga ctgccagcat cagcagcccg gcaataccgt
     1741 aactggtgcc ggacaaaggc ccccacagtg ccatcaggag aatggccgct gcagtacgcc
     1801 acagactgcg cgtttcacac cacgtcagca cctgtgccgc aactgcaaag gcaaacagga
     1861 tattcccctc ataccaggga aagccggcca gataatacgc gaactgggca atcacacccc
     1921 atccccacag ccggttaatg gccgactgcc ggatatgcgc atggcgggac agattcagcc
     1981 cccacaccag ggcaaaaagc ggaaaggctc cacgcccggc cagaaacatc cacgtctggt
     2041 ccagatgcag aatgcggttt gcatggtcca gtaccatcag caccagtgcc accgttttga
     2101 tgatatcccg ctgtccgggg gaccagacca gcaatgactg caaccaggta tcggtccggg
     2161 cagccagtga atcgttacgt gtcgtgttcg tgttatccgt tgtcatcagc gtttatcttc
     2221 ctgtatcagt ctccgcccag ggttttctct ttctgcaggt cacgaaccat atcccgctcc
     2281 atctgctgca gacgctcctg cagcaggttt tcccgggcaa cctcgcggac cgcttcccgt
     2341 tcccgttgtg gttcacgcag tacactctcc ggcagcgcgg cttcccgttc agttatggca
     2401 gcccggtcac gctcctgccc ggcaatttcc ctgacagcca gctctgtttt gccatccggc
     2461 aggtcaggtt tgttctctgc cattttacgg acaatttcat ctgcgcggcg ttccgtttca
     2521 cggcggatag cctcttcagc ctgccgctgt gccagtacct ctgccgtgac cggttcgcca
     2581 tttccggcac ccggctggac gctgttgtcc gggatatccc cccacacgcg gccaccggtg
     2641 atggcaccgg gattccacgg acggccttca ccggcgattc ttaccaccac accactgtca
     2701 ggattatcac gggcaatccg gacgccatcc tgcatattat cagccagcag gctctctccg
     2761 ttacggctgc cctgcagggc cacgaactgc gcatccccgc tgcctttcac acggccttca
     2821 ccactgaatc cccgcagccc gtttccgtca tccgtggtca gtgggttcag ccagatacct
     2881 gctgacttgc cgttacggtc aaacgccggc agtgccacat acggctgcgg atatttgcgc
     2941 cccggtgcaa taaaccgtgc aggactgtct cccccggcca gccccgcctg acgaagaacg
     3001 gcacggcctg ccgccacgtc ccgcagctcc cgcgccgtac tgaacagccg ctctgcattc
     3061 atgacctccc ggtccggttt cggctcaaac acatcgtggg ccgtaccttt ctgtacggca
     3121 ttgttgatgg catccgtcca gccctgacgg ttatcggtgt acacctgcac atgctgcttc
     3181 atacgcgaca gggccacgta ggctgactca aagccggcca tcagtttccg gttaccttcc
     3241 gtgccttcaa gcgcgatggc aaaggtttca cttgccccct gcgcaccgtg ggcggtgatg
     3301 gcataagcca ggtcaatatg ttgctctgcc cgctcctgac cggggcgaat cacccgggtc
     3361 tgctgtccgt ccgacagcgt gacactgtca ccggaaaccg ctgtcaccgt ccagacgctg
     3421 ttggccacat agccgcgctc ccgatcactc ttcgtgaagc gcatccggtc accggttccc
     3481 acccggatgg tgtccggggt gtacagtgtg acgccttcag ccaccgcctc ccggggcgaa
     3541 atcagccgcg tgttaccttc cgcatcctgc agggttatca gcccgtcatc cttactgata
     3601 ccggcaatcc ggtgatacgc attatccacc aggacaagtg cgtcccgatg tgtctcccag
     3661 gtggagagac gacgcagttc tccgtcacgt atattcgctg tgttcaggac aggcaccatg
     3721 acctgctctt tgcccagctc accggccttt tcccgtgcat catgaatcat gctgttcagt
     3781 acgcgccggt cctcattcag gtgtgtgaca atcagcgtct gctcccgtgc ttccggtgtc
     3841 ctgccggtat agtcgcggac aatggcttca tacagtgtca tggggatatc cgggaaagcc
     3901 tcgcctttca gcatcgcctt ctgctgcgct tctgccagtt tcgcttcctg gctgtgactg
     3961 aactccgtca cggagtgctc cggtgcccat gcgccctcca gacgtggcac ctgagacggt
     4021 ttcacgctct caagcccgga cagtgccctt tccacatccc ggttaatcag gctgtatacc
     4081 gcctcccgca gttccggcgt ctgacgcaca atctccttca tgatgaccac atcggcagca
     4141 ctgcgcgtct gctggagacg gaaaggctga ccgggcgcga tggcctgcag ctggtccgtg
     4201 tcaccgctgg ccacagcccg accgccaccg gccgcaatca gggcgtatgc ccgtgccatg
     4261 tcggtattgc ccaccattga gctctcatcg agcaggaaca gcgtgttgct gaaatccggc
     4321 gtttctccgc tgcgctgctg catctgcgtg tcatgcagaa aggacgccag tgtctgtgca
     4381 tccacgccgg cgctgcgcat ctccccgacc gcacggtgcg tggggcccag cccaacgaca
     4441 cggggacgct cactctccgg cagcatgttc acggctgaca tcaccgcccg gaactgtgtg
     4501 gtcttaccca cgccggcata gccctgcacc accgtgaaac ggtcggacgt ttccagtatc
     4561 atgcgggtgg cggcacgctg tcctgatgtc agtttctcca tgagttcgcc aggtactctc
     4621 tccatcagcg gcgtgaccgc ctccttacct tcgagaatat ggcgaagaat gctcttttct
     4681 gcctcatacg acgcacggga aaccagcagg cctgtgccat agccttttgc cacatccaca
     4741 tacagtaaat caccgcgttt tatctgcgca ttgatttccc ctcccaggtc gataaaactg
     4801 gttccttctg cagcaaacga cttcgcctct gtcagcaggt ccaccatgct gaaggccagg
     4861 tttttacttt ccagcacagg aagggccaga tgaatagcct gctgtgccgg cgtgtgaagc
     4921 ccggctttct gcagactgat agcggtttcc agcaatgtct caccggcacg cgcctttatc
     4981 tgctcagaaa ccaccgtaaa ggagggatga cgggcaagtt tttccgcagt acgggtttca
     5041 tccagtgaag aatacagccg gacatcacga ccgctgcggg ccagaccgtt cagggtggcg
     5101 ttgtccattg ccatctgtgt gacggaggca aaaaccttcg cactgtcgct gacggaatgc
     5161 ccgggcgttt ccacccagcc gttctccagc ttcagtgccg tgaacggtga atcagccaca
     5221 ggcagggtgg ccggttcagc ccgccccggc acaacaaccg tcatcgcatc ttcactgacg
     5281 gatgccacct gcaggcggtc accgccggag acgcggagtc cgggaatttt ccctgtcacc
     5341 ctcagacgct cgccgtctgc caccggcatt ttttccggcc ggaacagcga ccagctgctg
     5401 tccagagaag aaatacgcac cacctgcgtt tcaccctgcg catcccgcag ggtcaggctg
     5461 tgactctgcg ccgtcacccg gtctatcaca tagcggtcat gactgcgtgt ctccgggttc
     5521 cactgctcca tcaccattcc cgggcggtac atatcccgca gataacggct ccggctgtcc
     5581 agccagaccg gtgacagtgc cgtcatggtc acctcctgat gtccgagcac gccctgtgtt
     5641 ttcagctcac tgcgaatggc ctgtgtcagt atggcctgtt cccgtacccc gctgacctgt
     5701 gccacgctct cttctccggc tttcacgctg gccgcaaaat ctcctgccag ccgggcatag
     5761 cggacattac ggtccggttc actgatgatg gtggccggtc gctgttctcc cccctgccag
     5821 cgatatgtgt tcaccccggc atccttcatg gccatcagcg cactgcctgt accggttcgc
     5881 tgcccgctgt cggttatcag gacctgtacg ttatgacgtg cggcaccgtc cagcagggtt
     5941 aacgtctctt tcagggagag tttttcgccc tggtcaacga taacagtact gcccggcgtg
     6001 aaggccatgc cttccagcaa ctgacgacgt ccggttatca gctcaccgga cagccgttca
     6061 tcctgcttca ggttcatctg cgagcgacgg tcagcagcga taatctgcac ttcccgcccc
     6121 tgctcccggg ccatcatgac cagttcagcc acccgctcac gctgcccggc tgcaccgccc
     6181 tgcccggaca caatggccag cgacggacgg tcctgtgcca gaacactgac cgcatcgctg
     6241 taaccagccg tccgggggac acttttctcc ggatgtacgg tcacccggtt ctgtttcatg
     6301 atgtcacgac tgagtgcccg gacggacagc tcatcgagca catgaattcc tgatgtgaac
     6361 agccccttct cacggtcgag ggggataagc tgctcacgac tgatggtctc atcgataccg
     6421 gcgcgtgccc gttcaatcac accattttcc ggcggcagta taccgaccgt tctggcc
//
LOCUS       DBBGLQ010000179         6455 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.179, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000179 DBBGLQ010000000
VERSION     DBBGLQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.179"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            321..1877
                     /locus_tag="ACIYSB_005015"
     CDS             321..1877
                     /locus_tag="ACIYSB_005015"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HGC5431868.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSRLIAQLAMHGQTSV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFEAVVANDYNLSVSSYVEAKDNREIIDIDELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            1874..3067
                     /locus_tag="ACIYSB_005016"
     CDS             1874..3067
                     /locus_tag="ACIYSB_005016"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGC5431869.1"
                     /translation="MSELSYLEKLLDGVEVEWVTLGSMADIGTGSSNRQDESENGIYP
                     FYVRSKNILKSDTFEFDEVAIVIPGEGGIGDIFHYVEGKYALHQRAYRIRITTNAVDT
                     KFLYYFMSSSFKQYILTKSVGATAISIRKPMLEGFKVPIPSPDNPEKSLAIQSEIVRI
                     LDKFTALTAELTAELNMRKKQYNYYRDQLLSFKEGEINWTTLGNEELFHICAGGTPSK
                     SKAEYWDNGSIPWLKSESCNNESVYFAKDFISELGLKKSTAKLLPKNTTLIALVGATI
                     FKTAFLEFEATTNQNIASIKSTKENIITDKFIFYFLTNLYNTLKSEMRNYGMLNLTNL
                     RQFRIPIPCIAEQKRIVSILDKFDTLTNSITEGLPREIELRQKQYEYYRDLLFSFPKP
                     ETVSN"
     gene            3189..6305
                     /locus_tag="ACIYSB_005017"
     CDS             3189..6305
                     /locus_tag="ACIYSB_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HGC5431870.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     RNQGYEFIPVKSQSAMLANVREQLQNLNGVVFNDSEWRRFTEQFLDNPSDGILDKTRK
                     IHIDYICDFIFDDGRLENIYLIDKKNLMRNKVQIIQQFEQAGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAAN
                     KSTTCKPLRVATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSYFK
                     TNFSTDSKGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVAELEQRFPDPASIESEKEKKDFVKLFGEYLRAENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKLAELQTIRLPADRKIQDYRSAYNDIRDW
                     QRREKEAEKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKSEMIEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDAFFTFAQREQQREAEALIKEE
                     NLNEEAAKRYIRTSLKREYATENGTELNETLPRLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGKI"
BASE COUNT         1970 a         1411 c         1415 g         1659 t
ORIGIN      
        1 gtaaggcagg gaagaaagtg gtttttcaat tgcctcaatg gcatcagacg gattcataca
       61 tgtattcccg gtgaaaaaag acaaaacccc gcgcaccgga aaggtggcgg gggattcacg
      121 gagataatat ctgtctgaaa ttatttagaa tgcacctggt gcactgcaaa ggaaaggcaa
      181 tattccgttc ctcctccttg tcgtgaatac cccaggtatc tcacaatgta caccgactta
      241 ctccggggct gcctgaacca caagattgct gtagaattgc cctcaatgat tcaggtccaa
      301 accaggaaaa tggattaaaa atgacaagta ttcaacaacg agcagaactg caccgccaga
      361 tatggcaaat tgccaatgat gtcagaggtt ctgttgacgg atgggatttt aagcaatacg
      421 ttctgggcgc acttttctac cgttttatca gcgaaaattt ttccagttat attgaagccg
      481 gtgatgacag tatctgttat gcgaaactgg atgacagcgt aattactgat gacattaaag
      541 acgatgccat cagaaccaaa ggctacttca tctaccccag tcagcttttc tgcaacgtag
      601 ctgcgaaagc aaataccaat gacagactga atgcagattt aaacagcatc ttcgttgcta
      661 tcgaaagttc tgcttacggc tacccttcag aagcagacat caaaggtttg tttgctgatt
      721 tcgataccac cagtaaccgc ctgggtaaca ccgttaagga taaaaatgcc cgcctggctg
      781 cggttctgaa aggggttgaa gggttaaagc ttggcgactt taacgaacat cagattgacc
      841 tgttcggcga tgcctatgag ttcctgattt ctaactatgc cgcaaatgcc ggtaagtcag
      901 gcggtgagtt ctttacaccg cagcacgtct ccaggctgat tgcacaactg gctatgcacg
      961 ggcagaccag cgttaacaaa atctacgacc cggcagcggg ttccggctca ctgttgttgc
     1021 aggcgaaaaa acagtttgat aaccatatca tcgaagaagg ctttttcggt caggaaatca
     1081 accatacgac ctataacctg gcgcgtatga acatgttttt gcacaatatc aactacgaca
     1141 agtttgatat caagctgggt aatacactga ctgagccgca cttcagagat gaaaaaccgt
     1201 ttgacgccat cgtatctaac ccaccgtatt cggtgaaatg gattggcagc gatgacccga
     1261 cgctgattaa cgatgaacgt tttgccccgg ctggcgttct ggcccccaaa tccaaagctg
     1321 actttgcgtt tgtactacat gcgctgaact atctttcggc caaaggtcgt gctgcgattg
     1381 tctgcttccc gggtattttt taccgtggcg gcgcagagca gaaaatccgt cagtatctgg
     1441 tcgacaataa ctatgtcgaa accgtgattt cactcgcacc gaacctgttc tttggcacca
     1501 ccattgccgt aaatattctg gtgctgtcta aacataaaac ggataccaaa gttcagttta
     1561 ttgacgccag cgaactgttc aaaaaagaga ctaacaacaa tatcctgacc gatgcccata
     1621 tcgaacagat tatgcaggta tttgccagca aggaagatgt tgctcatctg gcaaaatctg
     1681 tcgcgttcga agccgttgtc gcgaatgact ataacctgtc ggtgagcagc tatgtggaag
     1741 cgaaagataa ccgcgaaatt atcgatatcg atgagctgaa tgctgaactg aaaaccacgg
     1801 tcagcaaaat cgaccagttg cgcaaagata ttgatgcgat tgtggctgaa attgaaggct
     1861 gcgaggtgca gaaatgagcg agctgagcta tctggaaaaa ctgttggatg gggttgaggt
     1921 tgagtgggta acattgggtt ctatggcaga tatcggtact ggaagttcaa atcgtcagga
     1981 tgaaagcgaa aatggaattt atccattcta tgttcgctca aagaatatct tgaagtcaga
     2041 tacctttgaa tttgatgagg tagcaatagt cattcctggt gaaggtggaa ttggcgacat
     2101 atttcactat gttgagggga aatatgctct acatcaaagg gcgtatagaa ttcgtatcac
     2161 aactaatgcc gtagacacga agtttttgta ttacttcatg tcatctagct ttaagcagta
     2221 catcttgaca aaaagtgtgg gtgctaccgc catttcgata agaaaaccca tgctggaagg
     2281 atttaaagtt cccattccta gtccggacaa cccggaaaag tcccttgcca tccagtctga
     2341 aatcgttcgg attctggata aatttactgc acttaccgct gagcttaccg ctgagcttaa
     2401 catgcgtaaa aaacagtaca actactatcg cgaccagttg ttgagtttta aagagggtga
     2461 gattaattgg acaactttgg gaaatgaaga gttatttcat atttgcgctg gaggaacccc
     2521 ctcaaaatct aaagcagaat actgggataa tgggtcaatt ccatggttaa aatcggaatc
     2581 atgtaataat gaatctgttt attttgcgaa agattttata agcgagctag gattaaaaaa
     2641 atccactgca aaacttcttc ctaagaatac cactttaatc gcacttgttg gtgcaactat
     2701 atttaaaact gcatttcttg agtttgaagc aacaaccaat caaaatatag ccagcataaa
     2761 gtccactaaa gaaaatatta ttactgataa attcatattc tatttcttga caaatttata
     2821 caatacactt aaatctgaaa tgcgtaacta tggaatgctc aatctgacaa atcttagaca
     2881 gttccgaatc ccaattccat gcatagctga acaaaaacga attgtatcta ttctcgataa
     2941 attcgacacc ctgaccaact ccatcaccga aggtctcccg cgtgaaatcg agttacgcca
     3001 gaaacaatac gagtactacc gtgatttact gttcagtttc ccgaaacctg aaactgtcag
     3061 taattaattg accattatta cagaccggat caccttaaca tccggtctgt atatagacta
     3121 ttttttacac gccggaagtc accattaact cccttccgac ccttgccaga cggcacaaag
     3181 gatgcgctat gactcatcaa acacacacca ttgctgaatc caataacttt atcgttcttg
     3241 ataagtacat caaagctgag caaacaggcg acagctacca gagcgaatcg gacctggaac
     3301 gtgaactgat tcaggacctg cggaatcagg gctatgaatt tatacccgta aaatcacagt
     3361 cagcgatgct ggccaacgtt cgggaacagc ttcagaacct caatggtgtg gtgtttaatg
     3421 acagcgagtg gcggcgtttc acggagcagt ttctggataa tccgagcgat ggcatcctgg
     3481 ataaaacccg taaaattcat atcgactaca tctgcgactt tatttttgac gacgggcgtc
     3541 ttgagaacat ctatttgata gataaaaaga atctcatgcg caataaagtg cagattatcc
     3601 agcagtttga acaggcaggc tctcacgcta accgttatga cgtcacgatc ctggttaatg
     3661 gcttgccgct ggtgcaaatc gaactgaaaa agcgcggcgt ggcgattcgt gaagcgttca
     3721 accagataca ccgttacagc aaagagagtt ttaacagcga aaattctctg tttaagtatc
     3781 tgcaactgtt tgtcatttct aacggcaccg atacccgtta ttttgccaac acaacaaagc
     3841 gcgataaaaa cagttttgac ttcaccatga actgggcgaa atcagacaac acgctgatta
     3901 aagacctcaa agactttacc gctacctttt tccagaaaca tactctgctg aatgttctgg
     3961 tgaactacag cgtttttgac agtagtcaga cgctactggt gatgcgaccg taccagattg
     4021 ccgccaccga gcgcattctg tggaaaatta agagttcctt tacagcgaag aactggtcaa
     4081 aaccggaaag cggtgggtat atctggcaca ctaccggttc tggtaaaacc ctcaccagct
     4141 ttaaagccgc gcgtctggca acagagctgg actttattga taaagtcttc tttgtggtcg
     4201 acaggaaaga cctcgattac cagaccatga aggaatatca gcgtttttcg ccagacagcg
     4261 tcaacggctc ggaaaatacc gcaggcctta aacgaaatct ggataaggac gataacaaaa
     4321 ttatcgtcac tactattcag aaactcaata acctgatgaa agcagaaagc gacctgcctg
     4381 tatataatca gcaagtggtg tttatatttg atgaatgcca ccgcagccag tttggagaag
     4441 cgcagaaaaa cctgaagaag aaattcaaac gctattatca gtttggtttt accggcaccc
     4501 ctattttccc ggaaaacgcc ttaggctcag aaacaaccgc cagcgtattt ggtcgtgaat
     4561 tgcattcgta tgtaattacc gatgcgattc gtgacgaaaa agtgctcaaa ttcaaggtgg
     4621 actacaacga tgtgcgacca cagtttaagt ctttagagac agaaactgac gagaaaaaac
     4681 taagtgcggc tgaaaatcag caggcgtttc ttcatcccat gcgtattcag gaaatcacgc
     4741 aatatattct gaataatttc cgccagaaaa cccaccgtac tttccctggc tccaaaggtt
     4801 ttaatgccat gctggcagta agcagcgtgg atgccgcgaa agcctattac gcgacgttta
     4861 aacggttaca agaggaagcc gctaataaat ctactacctg taaaccactg cgtgttgcga
     4921 caatcttctc ctttgccgct aatgaagaac aaaatgcgat tggtgaaata tccgatgaaa
     4981 cttttgatac cagcgcaatg gacagcagtg ctaaagagtt tcttgacgct gcaattcgtg
     5041 agtataacag ctattttaaa actaacttca gcaccgacag taagggtttt cagaactact
     5101 atcgtgattt agcccaacgg gttaaaaatc aggatatcga tctgttaatt gtcgtgggga
     5161 tgtttttaac cggtttcgat gctccaacat tgaacacgct attcgtcgat aaaaacttgc
     5221 gttttcacgg cctgatgcag gcattttccc gcaccaaccg tatttatgat gcaactaaaa
     5281 ccttcggtaa tatcgtcact ttccgcgatc tggaacgctc aaccatcgat gccataacgc
     5341 tgtttggtga caaaaacacc aaaaatgtag tgttagaaaa gagttatgca gagtatatgg
     5401 aaggctttac tgatgctgct actggtgaag ctaagcgcgg ctttatggca gtagttgcag
     5461 aactggaaca acggttccct gaccctgcca gtattgaaag tgaaaaagag aagaaagact
     5521 tcgttaaact gtttggtgaa tacctgcgtg ccgagaacat cctgcaaaac tatgatgaat
     5581 ttgccacgct gaaagccctg caacaaatcg atcttagcga tcctgttgca gtagaaaaat
     5641 tcaaagcaga acattatgtg gatgatgaaa agctcgctga attgcaaaca attcgtctcc
     5701 ctgctgatcg caagattcag gattatcgtt ctgcctataa cgatattcgc gactggcagc
     5761 gccgtgagaa agaagctgag aaaaaagaga aatcaaccac tgactgggat gacgtagttt
     5821 ttgaggtcga tttgctgaaa tctcaggaaa taaacctgga ttatatcctt ggtctgattt
     5881 tcgagcacaa cagacaaaac aaaggtaaga gcgaaatgat cgaagaggtc aaacgcttaa
     5941 ttcgttcaag cctggggaac cgtgctaaag agggcctggt ggtcgatttt attcagcaaa
     6001 cgaacctgga tgatttaccg gacaaagcca gcatcattga cgcattcttt acgtttgctc
     6061 aacgcgaaca gcaacgtgaa gcagaagcct tgatcaaaga agaaaatctc aatgaagagg
     6121 cggcgaaacg ctatattcgc acatctttaa aacgcgaata cgccaccgaa aatggcacgg
     6181 aattaaacga gacactgccc agactcagtc ctttaaatcc gcaatataaa acgaaaaaac
     6241 agactgtttt tcaaaaaatt agcgcattta ttgaaaaatt taagggggtg ggtggaaaga
     6301 tttagggaag gtgcgaataa gcaggtcatt tcttcccaag ctgactcgct gattaaaatt
     6361 tcgcggatct gggccgattt ttttcccgca aacacatcga atcggcctat ttaggctatt
     6421 ttttccacca tttctggcgt tatttccggt tttta
//
LOCUS       DBBGLQ010000180         6417 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.180, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000180 DBBGLQ010000000
VERSION     DBBGLQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.180"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..318
                     /locus_tag="ACIYSB_005018"
     CDS             <1..318
                     /locus_tag="ACIYSB_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431871.1"
                     /translation="VRELVIRSDIISVVRSIADENFTSEHMGYFERLTSDEKEVVFSD
                     YLRTLAEGGLTCTEKNLVKLTQDLYPIDATPDNIRKLSTDRDALNELHIDGMVLFITG
                     PAL"
     gene            403..894
                     /locus_tag="ACIYSB_005019"
     CDS             403..894
                     /locus_tag="ACIYSB_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431872.1"
                     /translation="MIVNKNRFMHKNGKRFKSIGRYLRFWQAVASSGLPMDMVIREKP
                     YQLMVALIALYTAIPVVTIVILSGQQSHKLELIAVHFSTIITMVFVLRRKMGKRWLDL
                     FEEKLAIYKPNDKLALSELHESIREKNGLDLQDLQDWYWKEKMTYEYHKEGKTTISYQ
                     KFK"
     gene            1061..1588
                     /locus_tag="ACIYSB_005020"
     CDS             1061..1588
                     /locus_tag="ACIYSB_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431873.1"
                     /translation="MNITDKELSSNTVRQYGWNLGEFNHSTPFTSHFIYITDYHKDNT
                     WMISLSQEDFNTTKILTSLSLDACVSMLGKILKKMSNKNGISQTEESEFAFLLTNYIK
                     QTLTFREWQRNADGNQRLHFLINIYGAKEDGGEVVLRPFIVNPDELMLTPADVVEFNS
                     QVINVDRQRHPEWFR"
     gene            complement(1639..2046)
                     /locus_tag="ACIYSB_005021"
     CDS             complement(1639..2046)
                     /locus_tag="ACIYSB_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431874.1"
                     /translation="MSPELVSGIARVAHEKLLSVLTECGTKKTKGTCLFASYLVCYLA
                     KTKGLDAVVRGGNGADDGGIFTESGGFGHYWCELNFEEVQYYIDITSEQFGFHPYIVK
                     RVNDITGWPRYIPGDQETVDSHLEQLLRDGYTE"
     gene            complement(2504..3175)
                     /locus_tag="ACIYSB_005022"
     CDS             complement(2504..3175)
                     /locus_tag="ACIYSB_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGC5431875.1"
                     /translation="MYRKVLALSALLISACSTDNPHKQSMHHNDFELSDSAVKIQIKS
                     GNKTISGSGTWIDEQHILTASHLWLDASKDYSLSIITRNGSQPATVINIDDPKNRDLA
                     LLKVEKNTIVKAPPNWKLPVCDNVLAPGESVWVLSGMYNTLSNTYASPDSTYYYKGIV
                     GSDGLTAFYQNGVSGSAVLNQSKSCLYGVVSQQDIKKINVYQIYITKITTNEIIRGFI
                     GYKNN"
     gene            complement(3220..3525)
                     /locus_tag="ACIYSB_005023"
     CDS             complement(3220..3525)
                     /locus_tag="ACIYSB_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431876.1"
                     /translation="MNVHLKYDTIKHYHFDWLTPAGDYPNSAVMLVGFRDGRWIIVQE
                     FGNDYSCFEGVLKNGDDLNTEPKFYSDLESVAVAAFGMMKQIYPQYQDSTLEEFLAG"
     gene            complement(3548..3865)
                     /locus_tag="ACIYSB_005024"
     CDS             complement(3548..3865)
                     /locus_tag="ACIYSB_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431877.1"
                     /translation="MADTYLPPGFKKCKSCQQVKPFEQFGKELKGKFGLKSKCRACIS
                     EKNKTYAAGPGAEVKTQNNRTYQAENKTELAEKMRVKRAKEKFGDRYNSYLASLESMK
                     KLK"
     gene            complement(4060..4335)
                     /locus_tag="ACIYSB_005025"
     CDS             complement(4060..4335)
                     /locus_tag="ACIYSB_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009655011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431878.1"
                     /translation="MKVKELIAMLNERDPEAIVLISGYETIGGTEVAEADLLIDMQSI
                     CLEQADNLTGNRKVVSSGGEDSVWLGWKDDYRTKVFLEDAQIPDQDE"
     gene            complement(4476..4673)
                     /locus_tag="ACIYSB_005026"
     CDS             complement(4476..4673)
                     /locus_tag="ACIYSB_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431879.1"
                     /translation="MKNDSLITRYKILKGERDESIFTIIIALVVKLGLVALAFYLLLG
                     STIDWSSWKTYAAMLIFGAFL"
     gene            complement(5660..5917)
                     /locus_tag="ACIYSB_005027"
     CDS             complement(5660..5917)
                     /locus_tag="ACIYSB_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431880.1"
                     /translation="MIVSEAIFYSSIISWVLYWWFIGNITKKVGMLVVPLTLTFGCYI
                     YAIHLDDSEQQTNLGWEQIGFITFIIVIMWAGKFVMKRLSE"
     gene            complement(5991..6305)
                     /locus_tag="ACIYSB_005028"
     CDS             complement(5991..6305)
                     /locus_tag="ACIYSB_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431881.1"
                     /translation="MNENSVILISLFWFFFGSSVVAGFMDASLYCWIFAAISALFLLS
                     VIQTPKELTVSILLAVLMASSAGVFILAYKVKTNVVSPIYLLKKPTAYMAEIDNRRIH
                     GY"
BASE COUNT         1968 a         1256 c         1230 g         1963 t
ORIGIN      
        1 gtgcgggagt tggtcataag gtcggatatc atctcagtag tgagaagtat tgcagatgag
       61 aatttcacgt ccgaacatat ggggtacttc gagcgactta cctctgatga aaaagaagtc
      121 gtgtttagtg attacctgcg cactctcgct gaaggcggtc ttacctgcac tgagaagaat
      181 ctcgtcaaac tgacgcaaga tttatacccg attgatgcaa ctcctgacaa cattagaaag
      241 ctatccacgg acagggatgc actgaacgaa ctacacatcg atggtatggt actttttatt
      301 accggccctg cgctttgatt atggagataa tacatgcggt gttatccctg ttaagggtta
      361 aggtaacatt cttctagaat tacagaaaaa tagaggtcta acatgatagt aaataaaaac
      421 aggtttatgc ataaaaacgg gaaaaggttt aagtccatcg gaaggtattt aagattttgg
      481 caagcagtag cgtcctctgg tttacccatg gacatggtaa tacgggaaaa accatatcaa
      541 cttatggtcg ctttgattgc tttatatacg gccatccctg ttgttacaat agtaatttta
      601 tctggtcagc aatcgcataa gttggaacta attgctgtgc atttcagtac aattatcacg
      661 atggtgtttg ttttgcgtcg aaagatgggg aaacgttggc tggatttatt tgaagaaaaa
      721 ttagccattt ataaacctaa tgataaactc gcgcttagcg aattacacga aagcatacgt
      781 gagaaaaatg ggctagactt gcaggattta caagattggt actggaaaga gaagatgaca
      841 tatgagtatc acaaagaagg taaaacaact ataagctatc aaaaattcaa gtagcatttt
      901 aaaatgacag gggagggcta ttgtgattta ttaagtcggc aaggttatta ggtaggggtg
      961 ttcaatatga taaattcttc gagtgaggtt atttcaagag ttgttctggg cctttgagat
     1021 agctaattat cattacagac tttatcgaaa ggtatgaatt atgaacatta ccgacaaaga
     1081 acttagctca aatacagtga gacagtatgg atggaactta ggagagttca atcactcaac
     1141 gccttttaca tctcatttta tttatatcac cgactatcat aaagataata catggatgat
     1201 ttcactttcg caggaggatt ttaatacaac caaaatttta acatcgttaa gtctagatgc
     1261 ctgtgtatcc atgctcggaa agatactaaa aaaaatgtct aacaaaaacg gtatctcaca
     1321 aacggaagaa tctgaattcg catttctttt aacaaactat attaaacaga ctttgacttt
     1381 tagagaatgg caacgcaatg ctgacggaaa tcaacgactg cactttctta ttaatatcta
     1441 cggtgcaaaa gaagacggtg gcgaagtggt tttaagacca tttattgtaa atcctgatga
     1501 attaatgctt actccagcgg atgtcgttga atttaattcg caggttatca acgttgatcg
     1561 gcagcgtcat cctgagtggt tccgttaact ggtcacaagg gttaagagcc tgaaaggctg
     1621 cgatagtgta tacgcagcct attctgtata gccgtcgcgt aataattgtt ctaaatgact
     1681 atcgaccgtt tcctgatcac caggaatata tcgtggccat cctgttatat cgttgacgcg
     1741 tttcactatg tatggatgaa acccaaactg ctcggaagtg atgtcaatat aatattgcac
     1801 ttcttcgaag ttcaattcac accaataatg gccaaagcca ccactctctg taaatatacc
     1861 gccatcatct gcaccgttac ccccacgaac aacagcatct agacctttag tttttgctaa
     1921 ataacataca agatagcttg caaaaaggca tgtgcctttc gtcttctttg tgccgcactc
     1981 cgtcaaaact gaaagtagct tttcatgcgc cactcgtgca atgcctgata ccaattccgg
     2041 ggacatgtct gctccaatga aattattaga tcgacttaaa caggtaatat gtaattaggc
     2101 attacgataa gtcgtgccct attggttggt acatcgtaag aaaagtaact tttctaatcc
     2161 cagcctgcac tccgggaatg attctaacct gaataaatca cgggggcagt gctggatact
     2221 tgtcaactgt atttggattc acaggctaac tatttttata cagtttaagt gaataaaact
     2281 tacttaaagc agcgaaccac gccgaaagtg taatgaggaa taccgccaat tcatcagaac
     2341 catttttgat agcactgagt agagcgatta gtccaaggaa caccgaaaga aagactagta
     2401 ttgcgggtaa aagtttatac atgatagacg ttccttttaa aggttcagat aggtagacca
     2461 acgcagataa cgtcggaatt gcctgttgta agattacgct catttaatta tttttataac
     2521 ctatgaatcc gcgtataatt tcatttgtag taatcttcgt aatatatatc tgatagacat
     2581 tgatcttttt aatgtcttgt tgactcacaa caccatataa gcaactctta gattggttta
     2641 acacagcgct tccactaact ccattttgat aaaaagcagt caaaccatca gaaccaacta
     2701 ttcctttata atagtaggtt gagtcggggg aagcatacgt atttgaaagc gtattataca
     2761 ttcccgatag aacccaaaca gactcacctg gagctagaac attatcacat actggaagct
     2821 tccaattagg tggggctttg acaatagtat ttttttctac ttttaaaaga gcgaggtccc
     2881 ggttcttagg atcatcaata ttgataactg ttgctggttg gctcccattc cttgttatta
     2941 tacttagact ataatccttg cttgcatcaa gccataaatg tgaagctgta agaatatgtt
     3001 gttcatcgat ccaagtacct gagccggaga ttgtcttatt tccagacttg atttgaattt
     3061 ttacagctga gtccgaaagc tcaaaatcat tgtggtgcat actttgttta tgaggattat
     3121 cggtggaaca agcagaaatc agtaaggctg aaagagctaa aacctttcta tacatagtca
     3181 gtttccttcg aataaggcga gtttggtttc tagaatattt tatccagcga ggaattcttc
     3241 taatgtgcta tcttggtact ggggatatat ctgcttcatc atgccaaaag cagcaaccgc
     3301 tacgctttct aagtcggaat agaatttagg ctccgtatta agatcatcac cattcttcag
     3361 aacgccctcg aagcagctat aatcatttcc gaactcttga acaataatcc agcgcccatc
     3421 acggaaacct acgagcataa ccgctgaatt aggatagtcg ccagcaggcg ttagccaatc
     3481 aaagtgatag tgctttatcg tatcatattt cagatgtaca ttcattattc ctccgttggc
     3541 gattacatta tttgagtttt ttcatggact ctaaagaagc gaggtaggaa ttatagcgat
     3601 caccaaattt ttctttcgca cgcttaacgc gcattttctc cgcgagctca gtcttgtttt
     3661 ctgcctggta ggtcctatta ttttgcgtct ttacttcggc ccctgggcct gctgcgtacg
     3721 ttttgttttt ctcgctaata cacgctcggc atttactctt gaggccaaac ttgcccttga
     3781 gctcttttcc aaactgttca aagggtttaa cttgctgaca tgatttgcat tttttaaagc
     3841 ccggggggag gtaggtgtca gccatgagct gctccagtag gtattgatta taggtgattt
     3901 taacttaaag acgacgtcac tttcaaataa atcgactact taacgcggat cttacataat
     3961 cctcgttatg cgcacttaag ttattgattc atatatggtt attttttaac atttacgttt
     4021 cacgtactga tagattgcct ttgaagccaa cgctgaccgt cattcatctt gatcaggaat
     4081 ttgggcatct tctaaaaaca cctttgtacg gtaatcatct ttccagccta accaaactga
     4141 atcttcacca ccggaagaaa caactttacg gtttcctgtg agattatcag cctgttctaa
     4201 gcatattgac tgcatatcaa tgagcaaatc agcttctgcg acttccgtgc cgccgatcgt
     4261 ttcatagccg gaaatcagaa caattgcctc agggtctctt tcgttcagca tggcgatcaa
     4321 ctctttcact ttcattttaa gataccagta gtttatttgt gtgcattgct gatggatttt
     4381 atagtgctgc cgaccttttt caaggtgctt ccgttgcttc caacgatcat gctaacgacc
     4441 ttgttgtctg acctggtatt gccgtggtca tccttttaga gaaatgctcc aaagatcaac
     4501 atggcggcat aggttttcca gcttgaccaa tcaatcgtgg aaccaagcaa cagatagaat
     4561 gccagggcta ccagccccag ctttactact agcgcgatta ttatggtgaa gatagactca
     4621 tcgcgttcgc ctttaagtat tttataccgc gtaattaaac tgtcgttttt catgtagcat
     4681 ccgttatagt tattaaagtc ctgcattaga agttgttatt tgccgaagtt accttgtatg
     4741 agttaactgt ggatccaaaa atacaaaata taagctgtgc caattgaaag gattagaaaa
     4801 gcacagttac taataaaatc tactaatgct tgtagcttct ttattctcac aattgctgga
     4861 ccgtgttcag tagaggtact actcagccct tttgttctgg cactctctgc ttttctgaaa
     4921 aaattgtaat gcaatcctga ccgcaagcaa aaccatgcca aaccagtcgt tattacgata
     4981 tttatgtaga aagcatattg aatcatttgt tcagtttcga agctttccat tgaaaaccct
     5041 ttgttgaaat gttgaataaa attattttta gtaaatgtaa gcaataaata gaacgaatat
     5101 taatagtgaa agatgcaatg ccaacatctg cttatgatgc cttatgagga tttcttcctt
     5161 cgcattcatt aaatcgtaca tctcatcaat atttttattg ctggtatatt cgagacgttc
     5221 atcacgaagt aatttgaact ttaccaatgc gacaagtgat ttaataataa agctcgtaag
     5281 cataacagtt atagacaaaa ataaaaaata ggccaaattc atatatcact ccatggagat
     5341 taatagaaaa atttataata agtaatctca tagagcatta cttgaatctt cttcccatac
     5401 taaggactca ccgagaaact tagttaaaga tgatttggca tactgtggtt cggaaaaaaa
     5461 acgtgggaac cagccgtcaa agctttgggt accattgtga cgattttcag gcgagactga
     5521 taagcggtac ccgtcttcgt aaggtgaaat gctaaatgtg tatccatcat ctgtctttga
     5581 gatcagcgtt ttgcatgtca tatgactgtt tgctcacaaa gttacttttc taagatatcg
     5641 aaaagtgtgg attagatcat cattcagata gtcttttcat cacaaacttt cctgcccaca
     5701 ttatcacaat tataaaggta ataaaaccga tctgctccca acctaaattg gtttgctgtt
     5761 cggaatcatc caaatgaatt gcataaatgt aacatccgaa ggtaagcgtg agtggaacaa
     5821 caagcatccc taccttttta gtaatatttc caataaacca ccaataaaga acccaggaaa
     5881 taatagaaga ataaaaaatt gcttctgaaa ctatcatatg taacctcttg aattagtcga
     5941 tgctgtgggt aaataaaccc ggaccgtcaa atgatgcaat tgcaaaatat ttagtaacca
     6001 tgaatccttc ggttatctat ttcagccatg tatgcagtag gcttttttaa gagatagatt
     6061 ggtgaaacga cattcgtttt gactttataa gccagaatga aaacacctgc ggagcttgcc
     6121 ataagtaccg caagaagaat agataccgtt aactcctttg gtgtttgtat caccgaaaga
     6181 agaaacaatg cgcttatggc tgcaaaaatc cagcaatata atgatgcatc cataaaacca
     6241 gctacaacac tcgatccaaa aaaaaaccaa aataaactta ttaagattac tgagttttca
     6301 ttcatgtctg ttccttgtta ttactaatta tattatgctt tatagttgac cgtcagacta
     6361 cccacgggtt ttgacgaaca aagtcacgca ctccatttat acatgctttt gagatga
//
LOCUS       DBBGLQ010000181         6414 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.181, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000181 DBBGLQ010000000
VERSION     DBBGLQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.181"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(100..354)
                     /locus_tag="ACIYSB_005029"
     CDS             complement(100..354)
                     /locus_tag="ACIYSB_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGC5431882.1"
                     /translation="MGIIAWIIFDLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            555..1289
                     /locus_tag="ACIYSB_005030"
     CDS             555..1289
                     /locus_tag="ACIYSB_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HGC5431883.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKLL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(1291..1902)
                     /gene="emtA"
                     /locus_tag="ACIYSB_005031"
     CDS             complement(1291..1902)
                     /gene="emtA"
                     /locus_tag="ACIYSB_005031"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGC5431884.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            2002..2916
                     /gene="ldcA"
                     /locus_tag="ACIYSB_005032"
     CDS             2002..2916
                     /gene="ldcA"
                     /locus_tag="ACIYSB_005032"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGC5431885.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            3011..4747
                     /locus_tag="ACIYSB_005033"
     CDS             3011..4747
                     /locus_tag="ACIYSB_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGC5431886.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQHHESNISWMFIVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFSLTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(5042..6387)
                     /locus_tag="ACIYSB_005034"
     CDS             complement(join(5042..5896,5899..6387))
                     /locus_tag="ACIYSB_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611354.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGC5431887.1"
                     /translation="MEKFSLEFKKVVSEKYLEGELSLKSVARMYGISPCTVRKWAYAY
                     REHGISILTGKKGRYSAELKLTVVKEVVDDCFSVREVAVKHGIPAFGTVCNWLEKYRK
                     YGEDAFIRKNKKSIPVPDKSAISAPPLPTLTKDEREELEQLRVENAYLKKLKALVQQK
                     TCSVQEKVSIVNELRQEWPLSRLLIVSGLPRSTFYYHVRRLAAPDRYQSARALVLKIY
                     HQHKGRYGYRRIRLACRNEGVLLNGKTVRKLMKELGISSLIRVKKYRSYKGEQGRICD
                     NLLKRNFDAKRPNEKWVTDVTEFKVNGKKLYLSPIMDLYNGEIISYNLATRPQPSMVQ
                     TMLTDALKQLSKDEYPILHSDQGWQYQMSRWQRWLKDSGIVQSMSRRGNCLDNAVIES
                     FFGTLKSECYYLNEYKNVEDLKRDIIDYINYYNQLRIKEKLGGLSPVQYRLSQAA"
BASE COUNT         1489 a         1594 c         1553 g         1778 t
ORIGIN      
        1 ctatattgat ttttttcacc cgtaaagcag gtgtcgagta tacgttctga tgtgaaagcg
       61 gatagcggca tctgtatcgc tatccgcctt atgaaaatct tattctcttc gcaggaggcg
      121 gaatatgccc agaacgagaa tagctcccac caccgccacc aggaagctgt gcagattaaa
      181 accactgatg gagccgccaa tgccaaacat ggtcgccagc cagccgccga ccaccgcacc
      241 gactatcccg agaatacagg tcaggaaaaa tccaccacca tcacgccccg gcatgattag
      301 cttggcgata atgccggcta tcaggtcaaa aataatccag gcaataattc ccatgttcag
      361 gccctcgttt cgatgaatcg gacgcaagaa atgcttacat caacgctcta agtataggca
      421 agggcgggat cgatgctggg cgtatgtcgg ctgatttaag aacaacatgt aaaaaatatt
      481 cacgcatttg tattaagttt tgttaactgt gaccgataaa ccaaagacag tttgtcagtc
      541 aggagttttt ccgcgtgagt cattaccatg agcagttcct gaaacaaaat ccgttagccg
      601 tcctgggcgt gttacgcgat ttgcacaaag ccgcaattcc tttgcgtctc agttggaatg
      661 gcgggcagct gatcagcaaa ttactggcaa taaccccgga taaactggtg ctggatttcg
      721 gcagtcaagc cgaagacaac atcgccgtgc taaaggcaca gcacattacc attaccgccg
      781 aaactcaggg tgcgaaagtc gagtttactg ttgaacaact acagcagagt gaatacttgc
      841 agcttccggc atttattacc gtaccgcctc ccaccttatg gtttgtacaa cgacgccgat
      901 atttccgcat ctccgcccca ctccatccgc cttatttttg ccagaccaaa ctggcggata
      961 acagtacgtt acgtttccgc ctgtatgatt tgtcgttagg cggcatgggc gcattactgg
     1021 aaacagcaaa gcctgccgaa ttacaagaag gcatgcgctt cgctcagatt gaagtcaaca
     1081 tggggcaatg gggtgttttt cactttgacg cccagttaat ctccatcagc gagcgcaaag
     1141 tgattgatgg caagaatgaa accatcacca ctccccgtct gagcttccgt tttcttaacg
     1201 tcagcccgac ggtggagcgg caattacagc ggattatttt ctctctcgag cgagaagccc
     1261 gggaaaaagc ggacaaagtg cgcgactgat ttacatcgcg tccagtgcct gctcaagttt
     1321 gtagatatag cgcggagcct gcggcgcagg gtgatttcgc gctacgtgtt cgaggaactc
     1381 gtcagcatcc aaatcgttga ttttgctgat cgcctttttc cgatctgacg agaaagtccg
     1441 tagcagcgca cctgccccgt tagcgtatga caccaccagc gcatattgca gtaccttcgg
     1501 atcttcaatg cctgccagcg ggccggtttc cagaatattc aggtaagccg cccccattga
     1561 aatattacgc tctggatttt tcagctcgct ggtcgtcggc tcaccactcc agcccatacg
     1621 gcgataaaca tcacgtccgg aggttgaagc ttttaactgc atcaaaccaa tggcattcga
     1681 tttactcacc gcgttcggat taccacccga ttcgatagcg ataatcgccg tgatcaattg
     1741 tggatcgacg ccccaggctg caccggcttt ctggcttatt ggcatccact gcatcgcacg
     1801 ttgcaccgga actttcgcgt tccacggcgg gttcgtatag tcatgctttg atgaacaacc
     1861 cgctaataac acaatcaaaa aggcaaacca tctcaatttc acttcttcta tccttatagc
     1921 ctggaatcgc cgttgaggtg acaacggcac acctaagcgg catgatatac atttcgtttc
     1981 tgttgtcagc aaggaattgc catgtctctg tttcacttaa ttgccccatc gggttactgc
     2041 attaaacagc acgccgcgct gcgtggtatc cagcgcctta ccgacgcggg gcatcaagtc
     2101 aataacgtcg aggttattgc ccgtcgttgt gagcgttttg cgggtacgga aacagagcgt
     2161 ctggaagatc tcaattccct tgccagactg actaccccca acaccatcgt gctggctgta
     2221 cgcggcggtt acggtgccag tcgtttactg gcagatattg actggcaggc actggtggct
     2281 cgccaacaac atgacccgtt gctcatttgc ggacatagcg attttaccgc cattcagtgc
     2341 ggtcttctgg cgcatggcaa tgtcatcacc tttagcggcc ccatgctggt agcaaacttt
     2401 ggcgcggatg aactgaacgc ctttaccgag caccatttct ggctggcgtt acgcaatgaa
     2461 accttcacca ttgaatggca aggggaaggc ccgacatgtc gggccgaagg cacgttgtgg
     2521 ggaggcaatc ttgcgatgct gatttcactg atcggtacac cgtggatgcc aaaaattgag
     2581 aacggtattc tggtgctgga agatattaac gagcaccctt tccgggtcga acgtatgctg
     2641 ttgcagctct atcatgcggg aattttgcca cgtcagaagg cgattattct cggtagcttt
     2701 agcggcagca cgcccaatga ttatgacgca ggttacaacc tggagtcagt gtacgcgttt
     2761 ttgcgttccc gcctgtcgat tccactcatt accggcctcg attttggtca tgaacaacgc
     2821 acggttacgc tgccgttggg cgcacatgcc attctgaata acacccggga aggcactcag
     2881 ttgacgatta gcggtcatcc tgttcttaaa atgtaaaaaa atcacgcttc agggctaagt
     2941 aaaacgctgt ctctgccgct aatatggtag ggtttatata agagacagcg taatcaggag
     3001 taaccgaccg ttggatgcca caacaataat tagccttttc atcttaggat ccatccttgt
     3061 caccagcagt atattactta gttcattttc ttcccgtctt ggcattccta ttctggttat
     3121 ttttctggcg atcggcatgc tggcaggagt cgatggcgtc ggcggtatcc cgtttgataa
     3181 ttaccccttc gcctacatgg taagtaacct ggcactggcg attattttgc tcgacggcgg
     3241 gatgcgcact caggccagtt cctttcgtgt ggcgttagga ccggcactgt cgctggcgac
     3301 gctgggcgtg cttatcacct ctggtttaac cggcatgatg gcggcgtggc tgtttaatct
     3361 tgatttgatt gaaggcttat taatcggcgc tatcgtcggc tctaccgatg ctgcagcggt
     3421 cttttctctg ctgggtggta aggggcttaa cgaacgtgtt ggctcgacgc tggaaattga
     3481 atccggcagt aatgatccaa tggcggtctt tctgacgatt accctaattg cgatgatcca
     3541 gcatcatgag agcaatatta gctggatgtt cattgtcgat attctgcaac aatttggtct
     3601 gggcattgta ataggtcttg gcggcggtta tttactactg caaatgatca accgcatcgc
     3661 cctgcccgcc ggattatatc cattgctggc attaagtggc ggtattctga ttttctcatt
     3721 aactactgcg ctggaaggta gcggtattct ggcggtgtat ctgtgcggtt ttctgctggg
     3781 taatcgccct attcgcaacc gctacggcat cctgcaaaat ttcgacggcc tcgcctggct
     3841 ggcgcaaatc gccatgttcc tggtgctggg gctgttggtt aacccaagcg atctgctgcc
     3901 tattgccatt ccggcgctca ttttgtccgc atggatgata ttcttcgccc gtcccctttc
     3961 ggtatttgct ggattgctcc ccttccgcgg tttcaatctg cgtgagcgcg tgtttatcag
     4021 ctgggtagga ttacgcggcg cggtgccaat catcctggcc gtgttcccga tgatggcggg
     4081 gctggagaat gcacgtctgt tctttaatgt cgccttcttt gtggttctgg tttcactgct
     4141 cttgcaggga acatcactct cgtgggcggc taaaaaagcc aaagtggtgg ttccgccagt
     4201 gggacgtccg gtgtcacgcg ttggcctgga tattcatccg gaaaatccgt gggagcagtt
     4261 tgtttatcaa ttgagtgccg ataaatggtg cgtgggcgcg gcactgcgcg atttgcatat
     4321 gccaaaagag acgcgtattg cggcactgtt tcgtgataac cagttgcttc atcctaccgg
     4381 cagcacccga ctgcgcgaag gcgatgtgtt gtgtgtaatt ggtcgggaac gcgatcttcc
     4441 ggcgctcggt aaactgttca gccagtcgcc gccggtcgcg ctggatcaac gcttctttgg
     4501 tgacttcatt ctcgaagcca gcgccaaata tgctgatgtt gcgctgatat atggcctgga
     4561 agacgggcga gaatatcgtg ataagcagca aacgctgggt gaaattgtcc agcagttgtt
     4621 aggcgcggca ccggttgtcg gtgaccaggt agagtttgcc gggatgatct ggacggtggc
     4681 cgagaaagaa gataatgaag tgttgaagat tggcgtacgg gtggctgaag aggaagctga
     4741 atcttaaatt taatgaagca tctatcgccg gttgcgatgc tttgctgaac gcagcctgag
     4801 cctgcaattg tttgtcgtcc ataatttaac cttcatttga tgttgaattg aacatatcaa
     4861 aatcaggcaa ttacacaaat ctatgtacag gctccatcaa gggtaaagcg tgatttatct
     4921 gaagtcgagt tcgagtcaac aatgaagcgt aagcgtttta tgtagaacgc ctgtgcggtg
     4981 agttcaggtt ccggggaaat agtgtccatc cttttgaact gcgccccaga tcttggacaa
     5041 cttatgcagc ctgactcaat cgatattgta ccgggctaag accgccaagt ttttccttta
     5101 tcctcaattg attataatag tttatgtaat caatgatgtc tcgctttaag tcctccacat
     5161 ttttatattc attgaggtaa taacattctg atttcaatgt tccaaaaaag ctctctataa
     5221 ctgcattatc aagacaattt ccccggcgag acatactctg gactataccg ctatccttta
     5281 accatcgttg ccaccgggac atctgatatt gccaaccttg atcgctgtgc agtatggggt
     5341 attcatcttt cgacagctgt ttcagcgcat ccgtaagcat agtctgcacc attgaaggtt
     5401 gagggcgagt ggcgaggtta taggaaatta tttctccatt ataaagatcc atgatgggcg
     5461 acaaatacag cttcttgcca ttcactttaa attcagtgac atctgtaacc cacttctcgt
     5521 tggggcgttt tgcatcaaaa ttgcgtttca ggagattatc gcaaattcgt ccttgttctc
     5581 ctttatatga acggtatttt ttaacgcgga tcaggctgct aatacccagt tccttcatca
     5641 gttttctgac ggttttacca ttaagtaaaa ccccctcgtt acgacatgcc agcctgatgc
     5701 gtctgtaacc atagcggcct ttgtgctggt gataaatttt cagcacaagc gctctggcag
     5761 actgataccg gtcaggagca gcaagccgtc tgacatgata gtaaaatgta ctccggggca
     5821 gcccggaaac aattaacaat cgggacaatg gccattcctg ccttagttca ttcacaatgc
     5881 tcactttttc ctgaacgttg agcatgtttt ctgctgaacc aaggctttca gcttttttaa
     5941 gtaggcattc tcgaccctga gttgttcaag ttcttctctc tcatctttgg ttaatgttgg
     6001 tagtggtggt gctgaaatgg cgcttttatc cggcacagga atactctttt tattttttcg
     6061 aatgaaagca tcttcgccat attttctata tttttcaagc cagttgcata cagtaccaaa
     6121 ggctggtatt ccgtgtttta ccgctacttc acgaacagaa aaacagtcat ccacgacttc
     6181 ttttactact gtgagtttta actcagcaga atagcgtcct tttttacccg taagtatgct
     6241 tatcccatgt tcccgatagg cataagccca ttttcttact gtgcagggac tgataccgta
     6301 catccttgct acagatttca gactcaactc tccctccagg tacttttctg atactacctt
     6361 tttgaactcc aatgaaaact tttccatgca tcctcagttt ttggtctggt gtct
//
LOCUS       DBBGLQ010000182         6328 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.182, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000182 DBBGLQ010000000
VERSION     DBBGLQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.182"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            60..632
                     /locus_tag="ACIYSB_005035"
     CDS             60..632
                     /locus_tag="ACIYSB_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGC5431888.1"
                     /translation="MQIGYIRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSARPG
                     LKKLLRTLSEGDTLVVWKLDRLGRSMKHLITLIEELREKGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAAREQGRIGGRRPKLTKEQHEQIARLIENGYS
                     RKQLAIIYDIGVSTIYRYHPVEKRQTQTEL"
     gene            complement(828..1065)
                     /locus_tag="ACIYSB_005036"
                     /pseudo
     CDS             complement(828..1065)
                     /locus_tag="ACIYSB_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904921.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1169..1495
                     /locus_tag="ACIYSB_005037"
     CDS             1169..1495
                     /locus_tag="ACIYSB_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HGC5431889.1"
                     /translation="MFADYENLAVVVITSLLSGTGVFLLGVRDGRISASLLNLASELF
                     TAVTAGLAGYGVAVSQEWPEGIIFCVVLIASNNGSEILQGLKSRASNVLNLLSVIANG
                     GKGGEK"
     gene            1495..1941
                     /locus_tag="ACIYSB_005038"
     CDS             1495..1941
                     /locus_tag="ACIYSB_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGC5431890.1"
                     /translation="MGNFGNYFIFIMAFVAIIDRFIFRRKSVEILSIGDAVVKESAIA
                     FPVSLRVKRSWVSNAQIEYWLQDLKNPSVVISGKTRPVDASKRGEREEYLLIDTKYLE
                     PTKWDLKVTLTNGNCRLNPLYRIFPINDCIERQFTIEKREGDWDVK"
     gene            1931..2551
                     /locus_tag="ACIYSB_005039"
     CDS             1931..2551
                     /locus_tag="ACIYSB_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431891.1"
                     /translation="MSNKNNYVLLNYDELNEKGLAKLVKEISKGGYKIARVIPASNGR
                     KKDGIMTRTFTLIGIDEQTMEVQVNDTGDISGIKLNGKTVPFKPVKTMSALGQSLAAL
                     FNRGATSFQKSLARKLARAAKNIDDGNKKRQGVKSNAQKLAEAKESRDALREDIANAN
                     DKLSKLQSKSDKVMQDTGNVKAALSQEVAKTRALKEEIARLEDEHD"
     gene            2544..4481
                     /locus_tag="ACIYSB_005040"
     CDS             2544..4481
                     /locus_tag="ACIYSB_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head protein"
                     /protein_id="HGC5431892.1"
                     /translation="MINNPLRINIQSTFADTIPGYMPSRWSSEELNEDDLIFEAATLE
                     DIELAYMGNEILVVSDEAMFESITTTRIRLAQTMRAFVRALNRGLNGTNIMAGTDEAG
                     TDENGRNCIGGAIIGRVRRIANIPVLTAQIPLTDGQSTSIIFHSPTADGAKIKSNDEL
                     VAFQFLLNKRNVTHVVAPIGGRDVSLSQVCQALSNLIERNSEKFKKAKERQDKMKADI
                     ESYLNEADKLAEERSIMIDQVDVAQRGLTEKRAALTEMQKKLDAQKAINEELQAKRDK
                     LLSAKGEKTKERAFSDQLRHVKHNLAIDGKTVLDNGAEVNYVTSGSDNFVTIVAPEGK
                     FSIDASAVKGGSLADAATKLLKAYREHNADKYKVDVLPGQQEDPEPPQTDPETNPVSQ
                     PESQDVGKYHYALQSRPAGVGAVPDGNKAVLDRPDQADQYYEYARHGIITYDRKLTDE
                     EVSQYELLYLPDEDELKDFAGQLVVSSMSKHIDGYVDLFGSDLKTFKAQVKILFRKAF
                     PNVAYPLGDGENLFIMDVYNALQNHSNEVTPEPELAPVIEPESHPQTAPEQQPTPEDA
                     EEAATEADQEADKALEYLKSVPVQFTSRDLTVISAELDHVQEAANALISAGRYDENES
                     TVGAAVDYLINILAEIQQGGA"
     gene            4481..4855
                     /locus_tag="ACIYSB_005041"
     CDS             4481..4855
                     /locus_tag="ACIYSB_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431893.1"
                     /translation="MTISVLDRLKLGKELSDLMQAQKTAPVLQRVAIGKQIVDLMLKL
                     GLGTAAQPMSEPQPQPQSDPVADEVPKIVTDFLGGVFTKSTQMEFIDALRGISNYVGE
                     FLTLEQAKEQTISWVKANGYAG"
     gene            4992..>6328
                     /locus_tag="ACIYSB_005042"
     CDS             4992..>6328
                     /locus_tag="ACIYSB_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431894.1"
                     /translation="MNSVIALVSSLERKETRLGRSSYVVTSKGAEVKTAFKVVDASSL
                     IISNNLDGMINPAFPEELQPRDRTRLSSKLQVNRIASNLRPAQLTDSGMSSHGAPIVG
                     PDNVVESGNGRSMGIWRAYEQGQADEYRQYLIDHAKEFGLNPDEISQMSMPVLVRERL
                     TDVDRAQFARDSNISDLQEMAASEKAYADAQFLTESVMALFNPSDDGNLLARSNDAFI
                     RAFLREIGDTATAGLLTADGRPTKQLIDRIQNAIFAKAYKDERLVRLVSEEPDPEMRN
                     ILTALNTAASDFAQMQSLSGDAHHDAVTGLVDGIEQVNGLDTQAIAALQEAINLVREA
                     KDNGQAVEEVIAQRGLFGDSTPEAEALALFIVANNRSAKRMGAAFKKMAQKINDELTH
                     KQQALGDMFGGGDVDLRRILSAVSDEIEGEFGEGKGLNFAMFENSTVLNNCSM"
BASE COUNT         1853 a         1314 c         1585 g         1576 t
ORIGIN      
        1 gtcttaatcc ctacctatac caactatgta tttttaacta aacaaagagg atgtttttca
       61 tgcaaattgg ctacattcgt gtgtcaacaa atgaccaaaa cacagattta caaagaaatg
      121 cactgaactg cgcaggatgt gaactaattt ttgaagataa aatcagcgga acaaagtcag
      181 ccagaccagg attgaaaaag ctgctcagaa cgttatcaga aggagatacg ctggttgtct
      241 ggaagctgga caggcttggc agaagtatga aacacctgat cacacttatt gaggagctgc
      301 gggaaaaagg tgttaatttt cgtagtctga cagacagcat tgacacctca acacccatgg
      361 ggcgtttctt ttttcacgtc atgggggctt tagcagaaat ggaacgtgaa ttaattgttg
      421 agagaaccct ggctggactg gcagctgcac gcgaacaggg acgcattggc ggacgtcgcc
      481 cgaagttgac aaaagaacaa catgagcaaa tagcgaggtt gatcgaaaat ggctacagca
      541 ggaaacagtt ggcaattatt tacgatatcg gtgtatcgac gatttatcgt tatcaccctg
      601 tagagaagcg ccaaactcaa actgagttgt aatacaaatg ccgcgtcgat gtagtttatc
      661 tcgagggaat agatacactt atcgcgcggc gcatcaattc gatgcatctt gtgtcggacc
      721 gaccactaat gaaaaccatc cctgtaatgt tgatcttaat tgcatcgtga gcgctgcatt
      781 ggtgtacaga tgtcgataga attagtggga ttcagaaggt ttattcatct ggcacgcagc
      841 gattacagca gcacaactaa attgccctca agccgctcta actgctatac gaccaaggct
      901 tcatctctgg aaaacatacg cagaaccttt tttaaccctt ggcgttcagc ctttttgcca
      961 ctcgctttgc cctcaaaaat tagctcacat cctgcgcttt caaggggggc gttgcaaagc
     1021 ggtgttttgg tcatttgttg aaatgcgaat gtatccaata agcatatgtc tcccttgtat
     1081 gctaaggata cgattctaga agatgtcctg taaggaaata acaaactgac taagttagtg
     1141 gcgcattaga tgttatttct ggaggggtat gtttgcagat tatgaaaatc tggctgtagt
     1201 agtcataaca tccctactaa gtggaaccgg cgtattcctt ctcggagtta gagacgggcg
     1261 catttctgcg tccctgctca atcttgcgag tgaattgttt actgcggtaa cagccgggct
     1321 tgcggggtat ggggtggcgg ttagccaaga atggcctgaa ggtatcattt tctgcgttgt
     1381 tctgattgcc agtaacaacg gtagtgaaat tttacagggc ttgaagtcca gagctagtaa
     1441 cgtcttgaat ctcttaagcg taatagcgaa tgggggaaaa ggtggagaga aataatgggt
     1501 aactttggaa attatttcat ttttatcatg gcatttgttg ccatcattga ccgtttcatt
     1561 tttcgtcgca agagtgttga gattttgagt attggtgatg ctgtagtgaa agaatccgct
     1621 atagcatttc ctgtttcgct acgtgttaag cgaagctggg tcagcaatgc tcaaatcgaa
     1681 tattggttgc aagacctgaa aaatccatct gtggttattt cagggaaaac gcgaccagtt
     1741 gacgcctcaa aacgcggtga gagagaagag tatttgctca tcgatacgaa atatcttgag
     1801 ccaacgaagt gggatctgaa agtcacattg actaacggta actgccgact gaatccgctt
     1861 tatcgcattt tccctatcaa tgactgcata gagcgccagt ttacgatcga aaagcgtgaa
     1921 ggagactggg atgtcaaata agaataatta tgttcttctt aattatgatg agttaaacga
     1981 aaaggggctg gcaaaactcg tcaaagaaat cagcaaaggt ggttacaaga tagcgagggt
     2041 gatccctgcc agtaatggca ggaagaaaga tgggatcatg acgcgcacgt ttacccttat
     2101 tggtattgat gaacagacaa tggaagttca ggttaacgat accggtgata tatctgggat
     2161 caaactgaat ggtaaaacag taccgttcaa gccagtaaaa acaatgtcgg ctttggggca
     2221 atctcttgcg gctttattta atcgcggagc aacttctttc cagaagtcat tagctcgaaa
     2281 acttgctcgt gcagcaaaaa atattgatga cggaaataag aagcggcagg gagttaaatc
     2341 taacgctcag aaattagctg aagcgaaaga atcacgtgat gcactccgtg aagatatcgc
     2401 taatgctaac gacaaactgt ctaaactcca gagcaagtca gacaaagtaa tgcaagatac
     2461 gggtaacgtt aaagctgcct tgtctcagga agtagctaag acacgtgctc ttaaagaaga
     2521 aatcgcgcgc ctggaggatg agcatgatta acaatccttt acgaattaat attcagtcta
     2581 cttttgctga tacgatcccc ggctatatgc caagtcgctg gtcttcagaa gaattaaacg
     2641 aagatgacct gatttttgag gcggcgacct tagaagatat tgagcttgcc tatatgggca
     2701 atgaaattct ggttgtgtct gacgaagcta tgtttgagtc cataactaca accagaattc
     2761 ggttagctca aacaatgcga gctttcgtcc gtgcgctgaa tcgtggcctg aatggtacga
     2821 atattatggc agggactgat gaagctggga ccgatgaaaa tggacgtaat tgtattggtg
     2881 gtgcgattat cggtcgtgta cgtcggatag ctaatatccc tgttttgacc gcgcagatac
     2941 cgcttactga cgggcagagt acaagcataa ttttccactc tccaacagct gacggggcaa
     3001 aaataaagag taacgacgag cttgttgcat ttcagttttt acttaataag cgtaacgtaa
     3061 ctcacgttgt cgccccgatt ggtgggagag atgtttcttt aagccaggta tgccaggcgc
     3121 tatctaattt gattgagcgt aacagcgaaa aatttaagaa agcaaaagag cgccaggaca
     3181 aaatgaaggc agatattgag agttatctca atgaagcaga taaactggca gaagaacggt
     3241 caataatgat tgatcaggtg gacgttgcgc aacgtgggct gactgaaaag cgtgctgctc
     3301 ttacggagat gcaaaaaaaa cttgatgccc agaaagccat caacgaggaa ttacaagcaa
     3361 aacgcgataa attgttgtct gctaaaggtg aaaaaacgaa agagcgagcg tttagtgacc
     3421 agctgcgaca tgtaaaacat aatcttgcca ttgacggaaa aacagtgctg gataatggcg
     3481 ctgaagttaa ttatgtcacg tccggcagtg acaatttcgt tacgatagta gcacctgaag
     3541 gtaaattcag tattgatgcc agtgctgtca aaggaggcag tttggcagat gctgcaacca
     3601 aattacttaa agcctaccga gagcataacg cagataaata taaagttgat gtgttaccag
     3661 gccagcagga agaccccgaa ccaccacaga cagaccccga gacgaaccca gtatcacaac
     3721 cagagagcca ggatgtaggt aaataccact atgcacttca atctcgcccg gctggtgttg
     3781 gtgctgtgcc ggatggaaat aaagccgtgc ttgatcgtcc tgaccaggct gatcagtatt
     3841 acgaatatgc tcgtcacggt atcattacgt atgaccgtaa actgacagat gaagaagtca
     3901 gccagtacga gttgctctat cttcctgatg aagatgagct gaaagatttt gcaggccaat
     3961 tggttgtgtc cagcatgtcg aaacatattg atggctatgt agacctgttt ggcagtgacc
     4021 tgaaaacatt caaggcgcaa gtgaaaattc ttttccgcaa agcgtttcca aacgtggcgt
     4081 atccacttgg tgatggggag aaccttttca ttatggacgt atataacgcc cttcaaaatc
     4141 attcaaacga agtaacgcca gagcctgaat tagcacctgt aattgagcca gaatcacacc
     4201 cacaaacagc accagagcaa cagccaacgc cggaagacgc tgaagaagcg gcaactgaag
     4261 ctgatcagga agcagataaa gcgttggagt accttaaatc cgtcccggtg cagtttacat
     4321 ctcgcgatct gacggtaatc agtgccgaac ttgaccatgt gcaggaagct gcaaacgctc
     4381 ttatcagtgc tggtcgatac gacgagaacg aatcaacggt tggcgcagca gttgactacc
     4441 tgatcaacat tctggctgaa attcagcagg gaggtgctta atgactatct ctgttttaga
     4501 ccgtctgaaa ttgggtaaag agctatcaga tttaatgcag gcgcagaaaa ccgcgccagt
     4561 attacaacgc gtcgccattg gtaagcagat tgttgatctg atgcttaaac tggggttggg
     4621 tactgccgct caaccgatgt cggaaccgca accacaacca cagtctgatc ctgttgcaga
     4681 tgaagttcct aagatagtga ctgacttcct gggcggcgtg ttcacaaagt ccacacaaat
     4741 ggaatttatt gatgcgctgc gtgggatctc aaactatgtt ggcgagttcc ttacgttgga
     4801 gcaggctaaa gaacagacca taagctgggt gaaagccaac ggttatgccg ggtaatcaaa
     4861 agggggcgaa atgccccttt cacttctctg ttctcacata aatcacactc tttttacact
     4921 cacagctttc tctatagttg gagtgtattt tgaacaaatc agttacttct gcgctttccg
     4981 aggccgcaga tattaacagc gtcattgctc tggtttcttc attagagagg aaagaaacac
     5041 gcctggggcg aagtagctac gtagtcacca gcaaaggtgc agaggtaaag acggctttta
     5101 aggtcgttga tgccagcagt ctgatcattt cgaacaacct tgacggtatg attaacccgg
     5161 ccttcccgga agaactccaa ccacgagatc gtacccggct atccagcaaa ctccaggtta
     5221 accgtattgc ctccaattta cgcccggcac aattaacgga ttccggtatg agcagccacg
     5281 gagcgccgat agttggtccg gacaacgttg ttgaatccgg caacggaagg agtatgggta
     5341 tctggcgtgc ctatgagcaa ggacaggcgg atgagtatcg ccagtacctg atcgaccatg
     5401 cgaaagagtt tggtctgaat cctgacgaaa tttcgcaaat gtctatgccc gtactagtgc
     5461 gagaaagact cacagatgta gaccgcgctc aatttgcccg cgattcaaac attagcgatc
     5521 tgcaagagat ggcagcaagt gaaaaagcgt atgcggatgc gcaatttctc actgagagcg
     5581 tcatggcgct atttaatcct tcagatgatg gaaatctgct ggcgcggtcc aatgatgcgt
     5641 ttattcgtgc gtttttgcgc gaaattggcg atacagcgac ggcaggcttg cttactgccg
     5701 atgggcgtcc cacgaaacag cttatcgatc gcatccagaa tgcaattttt gccaaagcgt
     5761 acaaggatga aaggcttgtt cggctagtgt cggaagagcc agatccggaa atgcgtaata
     5821 tcctgaccgc attaaatacg gcagccagcg attttgcgca aatgcagtcg ctgtctggcg
     5881 acgctcacca tgacgcagta acaggcttag tggacggtat agagcaggtt aatggcctgg
     5941 atacacaggc gatagcagca ctccaggaag caattaatct tgtacgtgaa gccaaagaca
     6001 acggtcaggc cgttgaagaa gtgatcgccc aaagggggtt attcggcgac agtacaccgg
     6061 aagctgaggc attggcgcta ttcatcgtag caaataatcg cagtgcgaag cgtatggggg
     6121 ccgcattcaa aaaaatggcg caaaaaataa acgacgagct gactcataaa caacaggcat
     6181 tgggcgatat gttcggcggt ggcgatgttg atctgcgtcg tattttgtct gctgtatctg
     6241 atgaaattga gggtgaattt ggcgaaggga aaggtctgaa ctttgctatg tttgaaaatt
     6301 ctacagttct aaataattgc agtatgga
//
LOCUS       DBBGLQ010000183         6262 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.183, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000183 DBBGLQ010000000
VERSION     DBBGLQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.183"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            768..1499
                     /locus_tag="ACIYSB_005043"
     CDS             768..1499
                     /locus_tag="ACIYSB_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGC5431895.1"
                     /translation="MNQPIHNAYWLSRFDSLLDSALAQHRAVSFIRVDLRFPEYMPAT
                     IMDSGPDSAVISRFFASLKAKIQAYQQHKRRASQRVHATSLRYFWCREFGEEENRKHY
                     HVILLLNKDTWCSLGDFSEPSSLAAMIQEAWCSALHLEPWQGDGLVHFSRWKPFRKPA
                     SSDAPPSSADTPLPGGCTDTRKAADKKPGEAAVLWIKRGDVAAMQKARNRASYLVKNK
                     TKQHNGSGQRNYGCSRGPGRLLDGR"
     gene            complement(1690..1818)
                     /locus_tag="ACIYSB_005044"
     CDS             complement(1690..1818)
                     /locus_tag="ACIYSB_005044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431896.1"
                     /translation="MRSPQRVLPGDGWADGLQAADEVDGTGEEPTLSGNIREEHSG"
     gene            1834..2409
                     /locus_tag="ACIYSB_005045"
     CDS             1834..2409
                     /locus_tag="ACIYSB_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431897.1"
                     /translation="MNTFSYFDDSMAPFWPSYQNKVIGVLDRALREQSGSRIRRILLR
                     LPWEPDNTFSSRRTWFGMAFIETVSDLMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDPRSPDCEELFRDLIWAF
                     SDFARDRRGVYDPEARHLAGNPWYPGSAGRR"
     gene            2477..2710
                     /locus_tag="ACIYSB_005046"
     CDS             2477..2710
                     /locus_tag="ACIYSB_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5431898.1"
                     /translation="MSTTTSHDSVWLRDDDPLIDMSFITRFTGMTDKWFYQLMSEGRF
                     PKPIKLGRSSRWYRSEVEQWMQQRIEASRGIAA"
     gene            2910..3419
                     /locus_tag="ACIYSB_005047"
     CDS             2910..3419
                     /locus_tag="ACIYSB_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431899.1"
                     /translation="MQITETVLSEPVEIRRFIQQAVDHWPRLLAVHFTLHSTEGNRDE
                     GQIHAFYRAFYRQLNQRSHAGYPLLPVVLRWLRETTESTGIRCLLLISQDLICPPGAG
                     LTADDADSHLVALLQQTGSTLTPEGHCTVVCCYRVVRAATPECAESFTTFRNTCLSLS
                     RPVMAGIIR"
     gene            3533..4480
                     /locus_tag="ACIYSB_005048"
     CDS             3533..4480
                     /locus_tag="ACIYSB_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGC5431900.1"
                     /translation="MTVSSRKEKNTPTPHDAAFRAFMASAEVARDFVELHLPAEYRQL
                     CDLSTLRLEPCTFVDQDLKQYACDALFSMKTISGEDGYIYCLAEHQSTENAFMAFRML
                     RYAVAAMQRHFEQYHELPLVIPLLFYHGERSPYPYSMNWLECFRNPELAAKIYTRPFH
                     LVDVTVLDDDEIMQHRRMGALTLLMKHSRSRDIMLQMDRLVQLIQTSLNEELAKALFH
                     YLLNGSEHVTVRFLQTLAERLPQHEGNIMTLAEQLKQEGEVKGFARGKLEGKLEGRLE
                     GQLEGKLETARNMLAEGMGLQTIMKITGLSEEQLKKISH"
     gene            complement(4502..4776)
                     /locus_tag="ACIYSB_005049"
                     /pseudo
     CDS             complement(4502..4776)
                     /locus_tag="ACIYSB_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4991..5998)
                     /locus_tag="ACIYSB_005050"
     CDS             complement(4991..5998)
                     /locus_tag="ACIYSB_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGC5431901.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQQRY
                     R"
     gene            complement(6024..>6262)
                     /locus_tag="ACIYSB_005051"
     CDS             complement(6024..>6262)
                     /locus_tag="ACIYSB_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HGC5431902.1"
                     /translation="IQLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGV
                     MSNRYFPHHPASLILQGESDVTLRYWSLRACMVE"
BASE COUNT         1475 a         1545 c         1635 g         1607 t
ORIGIN      
        1 tgtaaaacaa tccttcctgt ttaagcaatc aatctgctat tcgattttac tgatgtgacg
       61 tttaaccttt cctgagcttt ggttgtttgt ggtttttggt ttgtgtttcg acgttaagct
      121 gagaaaagta tctttacgct aaatcaatcc gaactttcct gaagtaatct gacgttcttt
      181 tcgatgtgct tacgacgatt ctgttaaacg actttctgga tgttttgata acgatcagcc
      241 tgaggtatgc attaacgatt cctgacgttt ttcttttccg tgtaaggtca ttctttttgt
      301 catgatgtgg tgtttttctt ctgtcgaaaa cgtgatgatt ggttttggtg ttttgattca
      361 gatgagattt acgttatcgg tggttaatcg tttaggtatg ttgaaaagta acatactcaa
      421 gataagaaaa tcgattctga tggggatgat cttgttacga cgttatctgt aaacaggtct
      481 taagcgtgtc atttcgcatc cgcgattgag cgatgttgtt aagctgcagg tttgatttgc
      541 attgtctgga tgcgtttctg gtgttttgtt gattcgtttc ccttatgact ttgtcgtttt
      601 taaatccatt cgctgttgtt gtgtgttttt accctgcgtg caggtttgtc ttttcgttcg
      661 taggagattc attcgcgatt ggcgttgctc aatggcggga tgccgtaaag ttttaccgca
      721 gtgattaatg atgtgaagtc aatccaaatc aacggagatc tctcatcatg aatcaaccaa
      781 tacacaatgc ttactggtta tcccgttttg acagcctgct cgacagtgcc ctggcgcaac
      841 accgtgccgt ctcgttcatc cgggtcgatt tacggtttcc tgagtatatg cctgccacca
      901 tcatggactc cgggccggat tcagcggtga tttcccgttt tttcgcctcc ctgaaagcca
      961 aaattcaggc ttaccagcag cataagcgac gtgccagtca gcgtgtgcat gcaaccagcc
     1021 tgcgttattt ctggtgccgg gagttcggag aggaagaaaa caggaaacat tatcacgtga
     1081 tattgctgct caacaaagat acctggtgct cgctcgggga tttcagcgaa ccgtcttcgc
     1141 tggcggcgat gatccaggag gcatggtgca gcgctctgca tcttgagccc tggcaaggcg
     1201 atggcctggt ccatttttcc aggtggaagc cttttcgtaa accagcatcg tctgatgccc
     1261 ccccttcttc cgctgacacc cctttgcctg gtggatgcac tgataccagg aaggctgcgg
     1321 acaaaaagcc gggtgaagcc gctgttctct ggatcaagcg tggtgatgtg gcagcgatgc
     1381 agaaagccag gaacagagcc agttatctcg tgaaaaataa aacgaagcag cataatggtt
     1441 ccggtcagcg taattatggt tgcagccgtg ggccggggcg tttgctggat ggcaggtgag
     1501 gtcggtaaaa cggtatccgg tgccaggggg gggatatcac ggtaagagag tcagccaatg
     1561 cccttatccc gtattctgat aacccgtcat gaagtcagca gcggtatctg cttccgcacc
     1621 cggaaacaat ctgtcggtaa aaccttaccg ccctgcttaa tgatgtgaac cgaagtcaac
     1681 ggagttcatt caccctgaat gttcttccct gatattcccg ctcagcgtcg gttcttcacc
     1741 tgttccatca acctcatccg cagcctgtaa cccgtcagcc catccatccc cgggcaggac
     1801 gcgttgtggg ctgcgcataa cggagcatat cctgtgaata ctttctctta tttcgacgac
     1861 agcatggcac ccttctggcc ctcgtatcag aacaaagtta ttggcgttct tgaccgtgcg
     1921 ctgcgtgagc agtccggctc ccggatacga cgtatcctgc ttcgtctgcc gtgggaaccc
     1981 gataatacct tcagcagccg caggacctgg ttcggtatgg cctttattga aaccgtcagt
     2041 gacctgatga atgcaacccc cggacgtgac ctctgctggc tcctgacacg tcatcctgaa
     2101 aagccggaat accacgtggt gctgtgcgtc aggcaggagt atttcgacgg ccctgaactg
     2161 gatcggctga ttctggatgc ctggagcaac gtgctgggct tcgcctcacc gggggaagcg
     2221 gcaccgtatc agaagcagat tacccgggat gtgctgctgg atccccgttc accggactgc
     2281 gaggaactgt tcagagacct gatctgggcg ttcagtgatt tcgcccgtga tcgccgtggt
     2341 gtgtacgatc cggaagcacg ccacctcgcc ggtaatccct ggtatcccgg atcagcagga
     2401 cggcgctgaa catcatgttc agccgttccc ccgcacttac acatccttcc atttcaccga
     2461 ggatattccg accatgttga gcacaacaac atcccacgac agcgtatggc tgcgtgacga
     2521 cgatcccctg atcgacatga gcttcatcac ccgcttcacc ggcatgaccg acaaatggtt
     2581 ctaccagctg atgagtgaag gcaggttccc gaagcccatc aaactgggcc gcagcagccg
     2641 ctggtacaga agcgaagtgg agcagtggat gcagcagcgc atcgaagcat cacggggaat
     2701 cgcagcatga aaggtcttgt gataccggtt cgctggcaat gtgcaaactg ccggcgctgg
     2761 tattgtggta atcccccctg cccctggtgc tggcggcctg ctgcaccgtc tgtcattcct
     2821 catccttcgc tgtaacaggc atcgtactgg tgccggcgtg ctttcctgcg taccggcacc
     2881 tgttcagaaa cattcaaaga gagcaaatcg tgcagataac cgaaaccgta ctttcagagc
     2941 cggtggaaat ccggcgtttt attcagcagg cggtggatca ctggccgcgt ctgctggccg
     3001 ttcatttcac gctccattca acagaaggaa atcgtgacga ggggcagatt catgcgtttt
     3061 accgggcatt ttaccgacag ctgaatcagc gaagccatgc cggatatccg ttactgccgg
     3121 tggtactacg ctggctgcgg gaaacgacag aaagcaccgg gatacgctgt ctgttgctga
     3181 tcagtcagga tctcatttgc ccaccgggag ccgggttaac agctgatgac gctgattctc
     3241 acctggtggc gttactgcag cagaccggca gcacactcac accagaaggg cactgtacgg
     3301 tggtatgctg ttacagagtg gtgcgtgcag ccacacctga atgtgctgag tctttcacga
     3361 cattcagaaa cacgtgcctt tcgttatcca ggcccgtgat ggccggcatc atccgctaag
     3421 ccattcgcga tgtcatcccc tccggtacag gtttcaggct gtggctgcac cggtgactga
     3481 aacgatcacg gctggtatta ttaaaaaaga gtgataccgg acagttgagt gaatgacggt
     3541 gagcagcagg aaagagaaaa atacccccac gccgcatgat gccgccttca gggcgtttat
     3601 ggcctccgca gaggtggccc gtgattttgt tgagctgcat cttccggcgg aataccggca
     3661 gttgtgtgat ctgtcgacgc tgcggctgga gccctgtacc tttgtggatc aggatctgaa
     3721 acagtatgcg tgtgatgcgc tgttcagcat gaagaccatc agcggtgaag acgggtatat
     3781 ttattgcctc gcggaacacc agagcacgga aaacgcgttt atggcgttca ggatgctccg
     3841 gtatgcggtg gcggcgatgc agcgtcactt tgaacagtac catgagctgc cgctggtgat
     3901 cccgctgctg ttctaccacg gcgagcggag tccgtacccg tacagcatga actggctgga
     3961 gtgtttcaga aatccggaac ttgctgctaa aatctacacc agaccgttcc acctggtgga
     4021 cgtgacggta ctggatgacg atgagataat gcagcaccgt cgtatggggg cactgacgct
     4081 gctgatgaaa cacagccgca gccgcgacat catgctgcag atggacaggc tggttcagct
     4141 gatacagact tcactgaatg aagagctggc gaaggctctg ttccactatc tgctgaacgg
     4201 cagtgaacat gtgacggtaa ggtttttgca gacgctggcg gagcggctgc cacagcatga
     4261 gggcaacata atgacactgg ctgaacaact gaagcaggaa ggggaagtca agggatttgc
     4321 gcgtgggaag cttgaaggta aactcgaagg ccgactcgaa ggacaactgg aaggcaaact
     4381 ggaaaccgcg cgtaatatgc tggctgaagg gatggggctt cagaccatca tgaaaatcac
     4441 ggggctgagt gaagaacagc tgaaaaaaat cagccactga agcattgagg cgtgctgaac
     4501 atcacgcctc gttcgttctg catgacagtg ttttgtttcc tgtgtattcg gtcagacgtt
     4561 ctcttttccc tgcggaaaca acgaagacaa caaccttatc gtctataacc tgatatacaa
     4621 ggcgaaaacc cgaagatttg agctttatcc tgtaacgctc gggcatacca cgcagcttat
     4681 tcacttctgc tcagggtgac ctggtactgc agccagtttc tttttcaacc ggttacgaac
     4741 tgtcgaaccc cgtattcccg ggcgtacggt caacagaggc ccctggttgt gaagccagtt
     4801 tcaccagatg aagtttaaac tcatttgaaa atgttctatg gggttctgcg gattaatgtt
     4861 ttttgttcca taacaggtgt ccactggtta aaaaggtggg cacctgcgtt accaatactg
     4921 tcttaatggc tacatacggc agtcagttta tgcttacacg gttctccata gaatattaat
     4981 aattaagaga ttatctatag cgttgttgta aactggcagg aagataacgc tgtaatgggg
     5041 atggcgtttc accttcaatc agttgcgaaa taagctcaaa gcagtgaaaa gcaagttggc
     5101 gattatcctg ttgtattgtg tcgattctta ccgaaagtga atcatacaag taatgatcat
     5161 caaaactcgc caggtggatt ttactgtcga gtaaattata ttggctcata tagcgcagaa
     5221 cgccttccag taatccacaa gcggcagtga ataaggcttt agggggacgt cctaaacgag
     5281 cgcatagcgc ggcaaacatt tcatagcctg agctcggatg ataattaccg tgaataatcc
     5341 attcaggttg tagcgttact ccggcttgct gcaagccttg tgtaaatcct tccagtcgat
     5401 cgcgagtcgg tgacaaacgg ggttgtcccc ccagaaaata gaattcatcc gggtgactcc
     5461 gtgcaatatc tgccaccaga gcggcggtgg gagaaacaga gtcagtaatg accaatggca
     5521 atgtactgcc gttcatgtgg cgatcgaaca acacaacagg taattgctcg ctaagttttt
     5581 gatagtcatt atcatgcagc atactggatg cgacgattaa cccatcaacc tggcgggcaa
     5641 tcatattgtt caccaccatt gtttcctgtg aaggattttc atcagtacag gatatgagga
     5701 gctggacacc tgcttcccga cacaatgttt ccagttcatg ggaaaatact gcaaatccgt
     5761 agttagtgat ttcaggaacg acgagaccaa tggtatgact gcggttgtcc cgtaatgagc
     5821 gggcatgaat gctgggctga taatgctgct gttgggcaat actcagcaca cgctcacgcg
     5881 tttcctgcgc aacacgtaac tctttgccac gaccattgag aaccagactg gcggttgctt
     5941 tcgacactcc cgccagttca gcaatgtctt taatagtaac gcgtttggtt tttctcacga
     6001 cgaggatccg taattcaaaa ccctcattct accatgcagg cgcgtagcga ccagtagcgt
     6061 aacgtgacgt cagactcacc ctgtaatatc agcgaagctg gatgatgggg aaaataacga
     6121 ttactcatta caccttcacc attattgatg aatatctcta cgctggaact gtcacagaga
     6181 atcttcagtg acgttacgct tcctcgccag taacgagtta gtttctcttg ccctgcaagg
     6241 cttctcctct gtagttgaat ac
//
LOCUS       DBBGLQ010000184         6240 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.184, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000184 DBBGLQ010000000
VERSION     DBBGLQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.184"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..3433)
                     /gene="rhsD"
                     /locus_tag="ACIYSB_005052"
     CDS             complement(<1..3433)
                     /gene="rhsD"
                     /locus_tag="ACIYSB_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HGC5431903.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYLYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTRSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSVFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVILWYYDESDRITHRTVNGEPAEQWQYDGHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGECQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEW
                     LTYGRGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTSTYTPAGQLQS
                     QHLNSLVYDRDYGWSDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTVWPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEVTWYGWDGDRLTTVQTDTTRIQTVYEPGSFTPLIRVETENGEREKAQRRSLAET
                     LQQEGSENGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQV"
     gene            complement(3500..5608)
                     /locus_tag="ACIYSB_005053"
     CDS             complement(3500..5608)
                     /locus_tag="ACIYSB_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGC5431904.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT         1152 a         1977 c         1676 g         1435 t
ORIGIN      
        1 ccacctgtct ggccagttgc tccaccgtca gcccgcactg cgcaagccac gcccggcttt
       61 cactgctcac gcggtctgcc cggatttctt cctccagcct gtccagcagc cgcaccagtt
      121 cagccgggaa caccacgccg tggccgttct cactcccttc ctgctggagc gtctctgcca
      181 ggctgcgccg ctgcgctttt tcccgctcgc cgttctctgt ctcgacccgg atgagcggcg
      241 tgaagcttcc cggctcgtat accgtctgga tacgtgtggt gtcagtctgc accgtcgtca
      301 gcctgtctcc gtcccagcca taccacgtca cctccggttt acgcgacagc gacatccacc
      361 ccgtcaggtc acgctcccgc cgccagaccc gttttgccat tcgccgtccc agcgggtcgt
      421 agaggtagcg gctctcgacc agtggctcgc catgctgtat ccgcgtgtag aacaccaggc
      481 ggtgctggct gtcgtagtgg tagtggtggg tccgctcgtc gtccgtccgt atcacacccg
      541 ccgggatgcg gtccgtcttc tccgtcagcc tgccgtattc atcgtggcgg tagacatagt
      601 gcgcatcctc cgcgatgcgg ttatccggcc acactgtgag tgtactgtcc gggtgcagct
      661 ccgggtccgg cagccggttg cccgccgggt ccgtggcata cgggatgcgg atgtccaggt
      721 ctggtgcgag ggtgcgcaca ctctccagcc tgcccgtggc gctgtagccg tattcccgcg
      781 tctgtcgcgg gccgctgatg cgcaccaggt cgccgttgtc actccacccg tagtcacggt
      841 catataccag gctgttcagg tgctggctct gtaactggcc tgcgggggtg tatgtgctgg
      901 tcagttcgta tgcggcatta ctgcctgcca tgctgccgaa gctgcgcacc gtctcacggt
      961 gcagcctgtc ccgcgtatac tcgaccagcg gcgtcccgcc cagcttcatt cccgccaggt
     1021 aaccgcggcc atacgtcagc cactccaccg gcggcaggct gtccggcgtg acgcggtttg
     1081 ccagcccctg ctcgttgtat gcgtgtttcg tctcatgctg ccacagcagt tcccccgtct
     1141 ccgggttctc caccgtctgg cattcgccgg tcaggcggcc tttatcgtca tagccatagt
     1201 ggacggcaac acggtggcct tcgctcaggt ggctgatgtc tgtcagccag ccgtggccat
     1261 catactgcca ctgctctgcc ggttcgccgt tcaccgtgcg gtgagtgata cggtccgatt
     1321 catcgtagta ccagaggatg acaagtccct catcctcact ctgtgtgagt tttccggtca
     1381 ggtcataatg ataacgttgc gtccgcccgt caaagccgcc ctgctgtacc agccggtcca
     1441 gcgcatcgta actgaagacg ctgtggctgc cgttctcgtt ggtcaggctg atgacacgtc
     1501 cggcagcatc gtactccata ctgcgcgtca gcccgccctg cgtggtgctg accgcctttc
     1561 cccacgcatc gtactgtgtc tcgctccggt tgccgtccgg ggtgataacg gcagtcaggt
     1621 cgcctgcggc gttgtattca taccgcgttt cacggccctg tgcgtctttc accgaggtta
     1681 accggccacg gttgtcatag cggcggtaaa ggctgatacc ttcctcgcgg tgaaccgccg
     1741 tcatctggcc gaagcggtcg tattcatagc gggtctggta gcccgagcag tcagtgaacg
     1801 ccagcaactg cccgtagcgg ctccacgtca tctgccgggt gctgcccgtc gcatccgttg
     1861 tcgtcgccgg taactcactg tgcgcgtcat cgtagcggta gcgtactgtc tccccgctgc
     1921 gcgatgtctc cgataccagc ctgcccggtt catcatattc ccggcggctc tccagcccgt
     1981 ccggggacac caccgccgtc agctggttcc cgtcgttata gtaaaatttc gtctcccgcc
     2041 cgtccggtgt ggtgatgtcc gtgatatcgc cggacaccac attcagaccg tactctgtcc
     2101 tccgtcccgc cgcgtccgtc tgcgccgtga gccttcctgc cgcgtcatac ccgctgcgcg
     2161 tgacgctgcc gtccgccagt tcttttttca ccacccgttt cagcccggcc ccgccttctg
     2221 tatgcagcac ctcacgccgg ttcaggctgt cggtgacggt gatgcggtcc tgctcataaa
     2281 gatagcggta gcttaaccct gccgggttca gttgctccac cacccgcccc gtatcgtcgt
     2341 agcggtagcg catctccggc cttcccgcgt aacggtgcgc caccatccgg cccgggtgct
     2401 gcgcgtcata cgtgaaagcg cgcacctgcg tattgctgcg gtcatatacc gccagcagtt
     2461 caccggcttc cgtatacgtg taccgcacca gtggcgcagc gggcaggctc tccgggtatg
     2521 ccgggtcgtg catcagccac accgccgaaa ggcggatacc cctgtcgggg ccgtattcgg
     2581 taccgggcag tgtgtcgggg aacgctgagg ctgagagagg gcgggaactg tcagaagaag
     2641 atagcgaaga ggtgcgggcc tcttccgcac gctgcgcctg cgtggtcagc accagacgga
     2701 actcccgccc ggcaccgtcc gtcacgccgg tgatttcccc ggccaggtcg cctgcggcct
     2761 cacgccggta cgtcagcgtc cgcccgaaac ggtctgccag cccggtaagc acccggtacg
     2821 gcggcagcgg cgctggcagt acgtcctcag cacccggcac ccgctcagac caccccagta
     2881 tccaccacgg cccctgtgcg ctgttggtcg ctaggtaaag atgcgggctt aaccggatat
     2941 ccggcggcag cgccccccac agccgcgcca gcgtatggcc gtccggctgt gctgccttac
     3001 caccgcgcac cagccacatt gactcactgc ggctgtacac cgcctccccc ggcagcagcg
     3061 gctcaaagtg aatgctccgc ccgccgttgt cgttgagtat cagtccgtca tcacgtagct
     3121 gtaagcggat atcagaaggc gctttccagc cggggccgaa aacgcccacc ggtgcaggtg
     3181 tcctggtccg gtagctgctg taggtgcggg agagaatgaa cggcagcggg ccgggcagcg
     3241 caaggtccgt ctcgccgggc agcaccttcg cccccagcag cggatttacc gggttgcccg
     3301 aagtcatccc gcccggacac accgagcacg ccacgccggt gggcgcgcca attcttacac
     3361 ctgccgaacc ctggacaatg ggaccgccat actgagtcat atctccctga cgcgccgctg
     3421 gttttccgct catgattcaa ttccttcttc tgtttatttt taatgctggt ttgttactga
     3481 ataccgaaca ggtacgggat cagtactctc tcatatccgg cgtatcctgc ttttcatcag
     3541 gggtcttcgg tggggatttc gaggctgcac agttccagtt aatcaacagt gagtcaccat
     3601 taacctgctc cttaccctgc aaatgaattt ttgtgccgtt gagaaaaatt tgtccgtcct
     3661 tcgtcagcac cagccttgcc ttaccacagc agagctccag atgctcaccg gcggagtaaa
     3721 tcgcggtctg cccggtatca tcctttttcg ttttaccaac ggtgaaggta acgttatttc
     3781 cgactttgac gtcataaccc agcccgaccc tcaacgattt atgcaaaccg atctgcgagg
     3841 actgcatcag agccaccgaa gtgttcataa tgccacctac cgtcgtctgg tacgccacgc
     3901 ccacggtcag cgcacgaaca agtccaatcg tttccacctg aacagaaccc actttaatta
     3961 tctggttact gcccaccgtc tctatctggt tgacacccac cgtctgtatc tgattgttgg
     4021 taaccgcaat catctgattg ttgccaatgg tttcagcatg gttgtttatc acatcagtgg
     4081 tgcggttatt cagcacctcg gtgttcatgt tcttctgcgc gtggatgtag acctgttctt
     4141 tccctgtcgc atcgtcaaac ttcagttcat taaacccgct gcccttatag gttttcgaac
     4201 gaatggtcat ctgcgtcttc gtccccggca ggctgccggg tgtgcggttt tcatggtggt
     4261 aggtacgccc cataataatc ggctggtccg ggtcgccgtt gaggaagtcc acaatcacct
     4321 cctgacccac tcgcggtatc gccaggttgc caaagccggt gcctgcccac gcctgtgcca
     4381 cacggatcca gcatgaactg tcctggtttg acgggttata gcggtcccag ttaaatttca
     4441 cccgcacgcg gccatgttca tcacagaaga tttcctcgcc tgccggtccc gtcacgacgg
     4501 cgctctgcgg gccgtcgacc agcggtttca gcaacggctg tggtcgccat gttctgtctg
     4561 ccgggatcac cgcaaaatgg ttctccagcg ccgtccccgc tccctgccgt cctggcaccg
     4621 cctgtggctg ttcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg
     4681 cctgcggatg ccccgtcagc acaattcgtc gtcccggcca tatctccggc gagcgactca
     4741 ttccccgcgc ggtttctgca ttgtttcgcc agccgtccat ctgccagcgg gcaaagttct
     4801 gcccgtgggc acccttgaaa cgtccggggt agtcatacac ttcatactgc gtgcgctggt
     4861 agtcctggtg ctggccttcc tgttcaaaac gtccggccca gccggggcgt ttaaaggtgt
     4921 agtctttggt caccacggaa gaagggcgga tttgtgcgct gtagcggaac tggctgatgc
     4981 agagggtgct cacctcggta cgggtgttcg ggttccatgg gatttcaaaa gattcgggca
     5041 gatggcggac tgtgtcgcac agcaccaggc tctggtcggt acttttgtaa gcatgctcct
     5101 cataaaagaa gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtctcac
     5161 cgtactggac acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccattcgg
     5221 tcaccccgtt ttcctgcaac atcgtgccga ggatgctttt gatgtcctcg ttctggaaga
     5281 tacggaagtt ctggcgcaga ccggcacgcc acagcggcgg gtgcaccttc atgctgtaca
     5341 gcatctggtt tttgtcgttc tccccgagtt caaaccaggt caccacgcct ttcacccggc
     5401 gctgtacttc atcgccctgc catatcgtca ggtaggccat tttgtccagc acctgcgcaa
     5461 attcaaggga gagaaactgc tggctgacca gggagagatc gagggaaaag agcgaagaga
     5521 gtgactggtt cagatgaaag gaaaccaccg caaaagcatc cggcggcagg ccgtccactt
     5581 ccagtgtgaa acgtaatccg gttgacatag caacctccgt gaaaatgaat aataatccgc
     5641 acatattgca tgtacaacat gtcgggcgga tgagataagc gagtcgacat ctggcgttac
     5701 cggggaatat cagaaaaatg tcatggttat gtgatgattt agtctgggat tttacttcag
     5761 agaaacgggt gtctgtttag tcaacaaaat aatcacaaac gcaacggatg ggttctcgac
     5821 gttattcacg acaggctcgt acattacagc gagtaataaa aaacctgata tgcaaagtca
     5881 aaccaatatt cataatgtct ttttattcac aacaataaat aattcaataa caatcattga
     5941 aatgaatgat ggtttgtata aggaatatac gctaccgccc caccctgata acatgatctg
     6001 cacattgcca tcttaacgtc gttaaacact agcttaatag tggatatttc aaattaggaa
     6061 tttattaaat tcacctgatg aatataataa gcaaatagca tagaaaataa tgacaactcc
     6121 cccgagggga ccatttccaa agcactgcac caccacctgg tgccggatga tcttgcttca
     6181 gtggtaagag tacctgatgc attgttccca aaatggtgtt ttcccctgcc cctccaataa
//
LOCUS       DBBGLQ010000185         6167 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.185, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000185 DBBGLQ010000000
VERSION     DBBGLQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.185"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..3822
                     /locus_tag="ACIYSB_005054"
     CDS             <1..3822
                     /locus_tag="ACIYSB_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HGC5431905.1"
                     /translation="GIVNISTDSLWNLKTSSTNAQLLQVGVLGTGELNITTGGIVKAR
                     DTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNNGTLNVEGGEVYLGVF
                     EPAVGTLNIGAAHGEAAADAGFITNATKVEFGLGEGVFVFNHTNNSDAGYQVDMLITG
                     DDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIANPGT
                     LDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFAGVAQLKDSTFT
                     LERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTIIFDTDIPAATLAEGYIS
                     VDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLNLLEHDDSHVGV
                     QLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAPGNGLY
                     VNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVRQVSLSNGQNDY
                     QGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTGQMGSTVLFKEG
                     ALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAEVSLDNTQGLGR
                     GNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVGQFNIDTGSALS
                     VNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILTLNNDSAAYQGT
                     TDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDMNVMPGGALRVAKTTIGG
                     NLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQG
                     NTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEK
                     NWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFSHRLHD
                     RLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDLAQWSS
                     NAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKTGAYVD
                     SWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNTWYVQPQA
                     QITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEA
                     NWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSD
                     TQGMLGVKYSW"
     gene            4045..4311
                     /gene="hipB"
                     /locus_tag="ACIYSB_005055"
     CDS             4045..4311
                     /gene="hipB"
                     /locus_tag="ACIYSB_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HGC5431906.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPESTEQQNLEW"
     gene            4311..5633
                     /gene="hipA"
                     /locus_tag="ACIYSB_005056"
     CDS             4311..5633
                     /gene="hipA"
                     /locus_tag="ACIYSB_005056"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HGC5431907.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK"
     gene            5945..6153
                     /locus_tag="ACIYSB_005057"
                     /pseudo
     CDS             5945..6153
                     /locus_tag="ACIYSB_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1678 a         1368 c         1716 g         1405 t
ORIGIN      
        1 gggatagtta atatttcaac ggacagtctc tggaatttaa agacatcatc tactaacgca
       61 caattgctac aggtcggtgt attaggcacg ggtgaactga atattaccac cggaggtata
      121 gttaaagcgc gtgatacaca gatagctctc aatgacaaaa gtaagggcga cgtgagggtg
      181 gatgggcaga actctcttct tgaaacattc aatatgtacg tagggacatc tggtacgggt
      241 acgttaaccc tgacgaataa cggtacgctg aatgtcgaag gtggagaagt ttacttaggt
      301 gtttttgagc ctgctgtagg aacgctaaac attggtgctg ctcacggtga ggcggcggca
      361 gatgccggat ttatcaccaa tgcgacgaaa gtggagtttg gtcttggcga aggcgttttt
      421 gtctttaatc ataccaataa cagtgatgcc ggctaccagg tcgatatgct gattacaggt
      481 gacgataaag acggaaaagt gatccatgat gcaggccata cggtgttcaa tgcagggaat
      541 acttatagcg gtaaaacgct ggtcaatgac ggcctcctga ccattgcgtc tcatacggca
      601 gatggggtaa cgggcatggg gtcgagtgaa gtaaccattg caaaccccgg tacgctcgac
      661 attctcgcat caacgaacag tgcaggagat tacacgctga ccaatgcgct caaaggcgat
      721 ggcttgatgc gagtgcagct gtcatcctcc gacaagatgt ttggctttac ccatgcaaca
      781 gggactgaat tcgccggtgt tgcccaactg aaagacagta ccttcactct ggaacgcgac
      841 aacaccgctg cgcttactca cgcgatgttg cagtctgaca gtgaaaatac cacatcggta
      901 aaagttggag agcaatccat tggtggactg gccatgaatg gaggtaccat cattttcgat
      961 acggatattc ctgctgcgac gctcgcggag ggatatatca gcgtcgatac gctggttgtc
     1021 ggcgcgggtg actacacctg gaaaggccgt aactatcagg taaacgggac gggcgacgtg
     1081 cttatcgacg tgcctaaacc gtggaatgat cccatggcga ataaccctct gacgacgctc
     1141 aatttgctgg aacacgacga tagccatgtc ggcgttcaac tggtgaaggc gcaaacggtt
     1201 attgggtcgg gtggctcatt aacgttacgt gatttacagg gcgacgaggt ggaagcggac
     1261 aaaacgttac acattgcgca aaacggaacg gtggtcgccg agggtgatta tggattccgc
     1321 ctcacgaccg caccaggtaa tggtttgtac gttaactatg ggctgaaagc gctgaacatc
     1381 catggtgggc aaaagctgac gttagccgaa catggcggag cctatggcgc aacggccgat
     1441 atgtcggcaa aaatcggtgg tgaaggggat ctggcaatca atacggtgcg acaggtttcg
     1501 ctttccaacg gtcagaacga ctatcagggg gcaacctacg ttcagatggg gacattacgt
     1561 accgatgcgg atggtgcact tggcaacacc cgggaactga acatcagcaa cgcggccatc
     1621 gtcgatctta atggatcgac gcagacggta gagacattca ccgggcagat gggttcgact
     1681 gttttgttca aagagggggc gctgacggta aataaaggtg ggatcagtca gggtgaactg
     1741 acaggtggcg gaaacctgaa tgttacaggg ggaacgctgg ctatcgaggg gcttaatgca
     1801 cgctacaatg cgttaaccag cattagccca aatgcggaag tcagcctcga taatactcag
     1861 gggttaggca gaggaaatat tgccaatgac ggtctgttaa cgctaaaaaa cgtgactggc
     1921 gaactgcgta atagcataag cgggaagggt atcgtgagcg caaccgccag gacagatgta
     1981 gagttggatg gcgataatag ccgctttgtg gggcaattca acattgatac aggcagcgcg
     2041 ctcagcgtca acgagcagaa aaacctgggt gatgcttccg ttatcaataa tggcctgctc
     2101 accatctcca ctgagcgtag ctgggcgatg acgcacagta tcagcggtag cggtgatgtg
     2161 acaaaactgg gtaccgggat cctgactctt aacaacgatt ccgcggcgta tcagggtacg
     2221 acggatatcg tgggggggga aattgctttc ggttccgact ctgccattaa tatggcaagt
     2281 caacacatta atatccataa cagcggtgtg atgtcgggaa atgtcaccac tgcaggtgat
     2341 atgaacgtta tgcctggggg ggcactgcgt gtcgctaaaa ccactatcgg cggtaacctg
     2401 gagaatggcg gcacggttca aatgaatagc gaagggggga aaccggggaa tgtactgacc
     2461 gttaacggca actataccgg aaacaatggc ctgatgacgt tcaacgcgac gctgggcggc
     2521 gataattcgc ccaccgataa gatgaacgtg aaaggcgata cccaagggaa cactcgcgtt
     2581 cgggttgata acattggcgg cgtcggtgca caaacggtca acggtattga actcattgag
     2641 gttggcggta attctgcagg taacttcgcg ctgaccaccg gaactgtcga agctggggct
     2701 tacgtctaca cgctggctaa agggaagggg aatgacgaga aaaactggta tctgaccagt
     2761 aaatgggacg gcgtaacgcc agcggataca cccgatccca tcaataatcc ccctgttgtg
     2821 gatccggaag gcccatcagt ttatcgcccg gaggccggaa gctatatcag caacattgcc
     2881 gcagccaact cgctgtttag ccatcgttta cacgaccgtc tgggtgagcc gcagtataca
     2941 gattcactgc attctcaggg gtcggcaagc agtatgtgga tgcgtcatgt cggagggcac
     3001 gaacgttcaa gggccggtga cggtcagcta aatactcagg ctaaccgcta tgtattgcag
     3061 ctaggcggcg atttggcgca gtggagtagc aacgcgcagg atcgctggca tcttggcgtg
     3121 atggcaggct acgccaatca gcacagtaat actcagagta atcgtgtggg ttataaatcg
     3181 gatgggcgca tcagcggtta cagcgctggg ctgtacgcga cctggtatca gaacgatgcg
     3241 aataagaccg gcgcttatgt tgacagctgg gcgctgtata actggtttga taacagcgtc
     3301 agttccgata accgttctgc tgacgactat gattctcgcg gtgtgacggc ctctgttgag
     3361 ggtgggtata cctttgaagc gggaacattt agcggcagcg aagggacgct gaatacctgg
     3421 tacgtccagc cacaggcgca aatcacctgg atgggtgtga aagattccga ccatacccgg
     3481 aaagacggaa cgcgcattga aacggaaggc gacggaaatg tgcaaacgcg acttggggtg
     3541 aaaacctacc tgaacagcca tcaccagcgt gacgatggta aacagcgtga gttccagcct
     3601 tacattgaag cgaactggat caacaatagc aaagtctacg ccgtgaagat gaatggtcaa
     3661 accgtaggcc gtgaaggtgc gcgtaatctc ggtgaagtac gtaccggggt tgaggcgaaa
     3721 gtaaataaca accttagcct gtgggggaat gtcggtgtgc aactaggtga taaaggctat
     3781 agcgatactc agggcatgct gggagtgaaa tatagctggt aaaccgtata agccgcatgt
     3841 cgagatggca tgcggcttaa tattgccgac ttcaaacggc gcatcaacgc cttatttaaa
     3901 tcctcctttt tatccgcgat cgcggatatc gcagcgttta tcccgtagag cggataagat
     3961 gtgtttccag attgacttat cctcactaaa ggataaaact tataatatcc ccttaagcgg
     4021 ataaacttgc tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa
     4081 ttggcgaatg caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg
     4141 aaaaaaattg gtattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc
     4201 acgctcacga cattttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac
     4261 gcgaaaaatg cctcgccaga atcaacagaa cagcaaaatc tggagtggta atgcctaaac
     4321 ttgtcacttg gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc
     4381 acacctttaa gtatgcaccg gagtggttag caagccgtta tgccagaccg ttgtcacttt
     4441 cgctgccatt gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc
     4501 tgttacccga tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca
     4561 gacaaccgtt tgatttattg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa
     4621 tacccgaaga cgaaaccgta acgcatccga taatggcatg ggaaaagctt actgaagcca
     4681 gacttgaaga agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag
     4741 aaaatgactt tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag
     4801 gcaatgactg gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga
     4861 ttggcgaaat caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt
     4921 attactgtct gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta
     4981 aagcgggaaa tgtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatgctgagc
     5041 gaacggtttt acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat
     5101 cggtgaaata tgaatcagat ggaggcccag gcatcgcgcg gatcatggct tttttgatgg
     5161 ggtccagcga ggcgctgaaa gatcgctatg attttatgaa attccaggtc ttccagtggt
     5221 tgattggcgc aacggacggt catgcaaaaa acttctccgt atttattcag gctggcggca
     5281 gttatcgact cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg
     5341 gaatacacat cagcgatctc aaactggcaa tggggcttaa cgcatccaaa ggcaaaaaaa
     5401 cggcaatcga taaaatttat ccgcgacatt ttttggcgac agcaaaggtg ctgagattcc
     5461 cggaagtgca gatgcatgaa atcctgagtg actttgccag aatgattcca gcagcactgg
     5521 ataacgtgaa gacttcatta ccgacagatt ttccggagaa cgtggtgacg gcagttgaaa
     5581 gcaatgtgtt gaggttgcat ggacggttaa gccgagaata cggtagtaag tgagatatgg
     5641 ggtttggtca ttgttaatga gcatgacaat catgaccgcc aacgactgga ttcttccttg
     5701 gtttatattg gtgcgcataa tgtatattat gttaaatcat gtctgagtga tgcgaagtgc
     5761 ttttctggta gtcgttattc gttcagggtt caagaactta agcctctcct ctttcataat
     5821 tggcctgtgc atggagaaat agcctggtta ttccctgccc catgctaaga gcgcagtgtc
     5881 tgtcgcatag acactgcata cgaaaacacc agagggtgct gttacttatt taaggacgga
     5941 tcaagcggga atgagtccac tccggtatca gaaatggcta cgtctcaatg aagtcaggcg
     6001 acagatgctg aatgaacatt acgatgtcac cacagcagcc tatgctgtcg gttacgaaag
     6061 ctatcccatt tcagtcggga atattcgcgg atgtttggag agtcacccaa gagagatatt
     6121 accaggttaa gaaagtctgt cggacagctt taataaaccc tgcactt
//
LOCUS       DBBGLQ010000186         6048 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.186, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000186 DBBGLQ010000000
VERSION     DBBGLQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.186"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(177..476)
                     /locus_tag="ACIYSB_005058"
     CDS             complement(177..476)
                     /locus_tag="ACIYSB_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431908.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYVPLLQKGSGDYTEDIVNDFL
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVEDFATTFWARKLDCIHNQFPLG"
     gene            complement(818..1102)
                     /locus_tag="ACIYSB_005059"
     CDS             complement(818..1102)
                     /locus_tag="ACIYSB_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGC5431909.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSIEIVNVVHTRRQFPVT"
     gene            complement(1103..1294)
                     /locus_tag="ACIYSB_005060"
     CDS             complement(1103..1294)
                     /locus_tag="ACIYSB_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HGC5431910.1"
                     /translation="MNTALSPMVSEFETIEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VERRMAERFAKIRKERSEQ"
     gene            complement(1324..1722)
                     /locus_tag="ACIYSB_005061"
     CDS             complement(1324..1722)
                     /locus_tag="ACIYSB_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5431911.1"
                     /translation="MGVLEISALRIRKLLLVTGWSQAELARRIGITQQSVQRWVCGIS
                     SPTAANLDKLSEATGYPQYWFFLPLNEEKNDRIQDIIKITPRQKELLQTFEAFPEEDQ
                     EQMLQEMKDKKELMDRTIERWLAARKGHRA"
     gene            complement(1757..2128)
                     /locus_tag="ACIYSB_005062"
     CDS             complement(1757..2128)
                     /locus_tag="ACIYSB_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431912.1"
                     /translation="METREIRRLRLKEWFKDRTLPPKEKSYLSQLMSGKATFGEKAAR
                     RIEQTYGMPGGYLDIEPEDITESPSSRAIALTPNQLELLQIFSAFPDNEQQEIIKELR
                     NKKEAMEDFVARWLAQQGRRA"
     gene            2232..2519
                     /locus_tag="ACIYSB_005063"
     CDS             2232..2519
                     /locus_tag="ACIYSB_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5431913.1"
                     /translation="MTLKEFIKSLKTGEAKKFAANLGVSPSFLSQMASGSSPVSPVRA
                     LMIEAETAGHVTRRELRPNDWDLIWPEYTGITPPSSHKYELNTKKVDGVDS"
     gene            2516..2941
                     /locus_tag="ACIYSB_005064"
     CDS             2516..2941
                     /locus_tag="ACIYSB_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGC5431914.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVEMRERLVRDADDFVAVAIAGFNQINRGGPAGNAVAVH"
     gene            3013..4083
                     /locus_tag="ACIYSB_005065"
     CDS             3013..4083
                     /locus_tag="ACIYSB_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HGC5431915.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEMIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPDQPAGTTPEADSAYALKADSGAVQQAMTAGSEQSHQL
                     QQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPQVMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPTGGT
                     NNQRCVNHISEPDTEIPPGFRG"
     gene            4090..4836
                     /locus_tag="ACIYSB_005066"
     CDS             4090..4836
                     /locus_tag="ACIYSB_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGC5431916.1"
                     /translation="MKNIAAGGVLERIRKLAPQHVTAPYRTAEEWREWQLAEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFANYWVQNDGQRYALSQAKSIADVLRTGCT
                     NFVFSGKPGTGKNHLAAAIGNHLLAKGRSVIVVTVADVMLALHGIYDNKNSGEKFLQG
                     LCDVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTAAMRSVGMLTNLNHTAMSTLLGGRV
                     MDRMTMNGGRWVNFNWESWRANVGQPGIEK"
     gene            4860..5630
                     /locus_tag="ACIYSB_005067"
     CDS             4860..5630
                     /locus_tag="ACIYSB_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGC5431917.1"
                     /translation="METVLDALKAMGKATSVELATRLDISREEVLNELWELKRNGLVD
                     KTGHIWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFISQEGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNRNGKFSYCMPGDNLPAEPKAAPVTKTAGKA
                     FPQPAGVALPVQEAATQEEITTDTVADIVQSLPSFTETRADDLVLPSLHMANRELRRA
                     KNHVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            5646..6040
                     /locus_tag="ACIYSB_005068"
                     /pseudo
     CDS             5646..6040
                     /locus_tag="ACIYSB_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
BASE COUNT         1585 a         1374 c         1609 g         1480 t
ORIGIN      
        1 gggaaccccg ccatgcttac cgccgcgcca tttcgcggag tgccacaacc ggaagcggac
       61 ggtcgacgaa aatttaacga caggctatat atgaaccagc tacctcgccg tgcgctttcg
      121 tgttgtgtgc ctgcttttaa ccacgtcagg cgaggtggta tcctctgagg ggtctgttac
      181 ccgagaggaa attggttatg aatacaatca agttttcttg cccagaatgt ggtggcgaag
      241 tcttcgacac atcctttaag ccgcagggct ctgacagttt cgcgggagcc atctgcaaaa
      301 attgtggtca ccttgtaact gaagatgagt cctcgcaatt cgatgaggaa atcgttgaca
      361 atatcttcgg tgtaatcacc ggagcctttc tgaagtaaag gcacataccg cttagttacc
      421 gctctgataa ttcttacctg cccggcaatg gcgctgatgt caatataaag cgccattgct
      481 gcttctttgc cgatcccggg atgccttcca ttctgatgtt tgacttcgcc cactgagaaa
      541 tcctctgttt ccccttaacg ccggggtagc ggaacaaaaa cctgctgcat agttaaagtt
      601 aaaccctgcc gtcatgttca tacgcctcgg gctggctact taacccctga ccactgcctg
      661 gtaactcgaa gtattgccct gcgttctgtg gggcggggtg ggttggtgaa attagtttat
      721 acaaaaataa acttaacagt caatctgcaa cttaactaaa cagtaaaaaa taacactctc
      781 tttgagagtg tttaaggctg tgttgatggg cagagggtta tgttacaggg aactgtcggc
      841 gggtatggac tacgtttact atttcaatac ttgaggttgt cacgcggtac agaattatat
      901 aattggggtg agccacaatc tcacgtaagc ccggcactct gtcgcttggg gggtataaat
      961 acggatgttc ggataatggc agcacacacc cccttaatcg ctgccataag cgttctgccg
     1021 catctatgtc gaaacgagca atgtaactag ttatatcatc caggtcggta tctgcgcttt
     1081 caagccataa cacaggtaac atttactgtt cactccgttc cttgcgtatt ttagcaaagc
     1141 gttctgccat tctgcgctca acttcgtcat ggggaattgc tgggcgcgga tctgcaaggc
     1201 ttgcagctac tttggctcgc agccattcgt tgtaactgtt ttcttgttca atagtttcaa
     1261 actcagaaac cattggtgaa agggccgtat tcatggcaca cctccgtctg tgtatggtgg
     1321 tcattatgcc cgatgccctt tccgggcagc gagccatctt tcaattgtgc gatccattaa
     1381 ctcttttttg tctttcatct cctgaagcat ttgctcttga tcttcctctg gaaatgcctc
     1441 aaatgtttga agtagctcct tttgcctagg agttattttt attatatctt gtattctgtc
     1501 atttttttct tcatttaacg gtaagaagaa ccaatactga ggatatcctg ttgcttcaga
     1561 tagtttatct agattagctg ctgtagggct tgagatacca caaacccatc tttgaacgga
     1621 ttgttgagta atgccaattc tgcgcgcaag ttctgcctgg ctccagcctg ttaccaacaa
     1681 aagcttccta attctaagag cactaatttc gagtacaccc atctgatata tcttgttttt
     1741 aagagtatgc ttcccatcag gcgcggcgac cttgctgcgc caaccaacgt gcgacaaaat
     1801 cttccattgc ttcttttttg tttcttagtt cttttatgat ctcttgctgt tcattatcag
     1861 gaaatgcact aaatatttgt agcaattcca gctggttagg ggttaatgct atagctcttg
     1921 atgatggaga ctctgttatg tcttctggct caatatcaag ataaccccct ggcatcccat
     1981 atgtttgttc aatacgtcta gctgcttttt cgccaaacgt agccttaccg ctcattaact
     2041 gagataagta acttttctct ttgggtggca aagttctgtc tttgaaccac tctttgaggc
     2101 gtaaacgccg aatctctctt gtttccatac atgcattatc tttagtaatt gcgaaataag
     2161 caaatacttg actggtttat ttagttacgt ataaactttt gcttgcacta aagtatgttg
     2221 agatcgaaat catgactcta aaggaattca tcaaatcatt aaaaactgga gaagcaaaaa
     2281 aatttgctgc caaccttggt gtgtctccgt ccttcttgtc tcaaatggca tctggcagtt
     2341 caccagtgtc tcctgttcgt gcgttaatga tagaagcaga aactgcaggg catgttacaa
     2401 gaagagagtt gaggccgaat gactgggatc ttatatggcc ggaatacacc ggaattacgc
     2461 ccccttcaag tcataaatat gagcttaaca ccaaaaaagt tgatggggtt gattcatgaa
     2521 aatcaaacat gagcacatcc gcatggcgat gaatgcctgg gcgcatccgg acggcgaaaa
     2581 agtaccggct gcgaaaatta ccaaagcgta tttcgaactg ggtatgacgt tcccggaact
     2641 gtacgacgac agccaccctg aagccctggc gcgtaatacc cagaaaattt tccgctgggt
     2701 agagaaagac acccctgatg cagttgaaaa aattcaggcg ttgttaccag cgatcgaaaa
     2761 ggcaatgcca cctttgctgg tggccagaat gcgcagccac agttcagctt attttcggga
     2821 gctggtggag atgcgggaac gactggtgag agacgctgat gattttgtcg cggtggcaat
     2881 agctggcttc aaccagatca accgtggtgg cccggccgga aatgctgtgg ctgtgcattg
     2941 atcggcaata ttcataccgg atctctttcg gtaatacgtg attaaaaaga ttcggtatct
     3001 gaagaggtta gtatggctaa tgcctggctc agattatggc atgacatgcc aaatgacccc
     3061 aagtggcgaa ccattgcccg ggtgtcaggt cagccaattg caacagtgat ggctgtgtat
     3121 atccacctcc tggtgagcgc gtcacgaaat gtcacgcgag gtcacattga tgtcacgacc
     3181 gaggatttgg caagtgcgct cgatgtgaca gaagagatga ttgattcaat tttgcagacg
     3241 atgcaggggc gggtacttga tggtgattta atcactggat gggaaaaacg ccaggtgctg
     3301 aaagaggaca acggcaatat ttcgcaaacc gcaaaatctc ctgcagagcg caagagggcg
     3361 cagcgagaga gggaaagaaa gcgggaacaa aatggcgatt gtcacggcgc gtcacgaaat
     3421 gtcacgcaca tgtcacgacg agtcacgaca gataaagata cagataaaga tacagatcaa
     3481 gaagatcaaa acactatggt ccatggcgtg aaaaacgcca cgaaccaggc tggggatgtt
     3541 cagaccgtca atcctgatca gcccgcaggc acgacaccgg aagccgattc agcgtatgcg
     3601 ctgaaagccg attcgggcgc tgtgcagcag gcgatgaccg cagggtcgga gcaatcacac
     3661 caactgcagc agcctgaagc cgattccgcg attcagcggg aagccgatcg ggtagtcccg
     3721 gaaaacaccg ggcagcctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
     3781 cgggaatacc cgttgcgtgc cggggcaaac ccgaagaaat ccgctttcag tgcctggaag
     3841 gccagattac gcgagggggt gccaccgcag gtcatgctgg atggtgtgag gcgttacgcg
     3901 aggtacctgg cggcgaccgg gaaagcggga acggaatttg ttcagcgggc gacaacgttt
     3961 tttggacctg accggaattt tgaaaacccc tggttgctcc cgactggcgg gacgaacaac
     4021 cagcgttgtg tgaatcatat ttccgaaccg gataccgaaa ttccaccagg tttcaggggg
     4081 tgaaggatta tgaaaaatat agcggcagga ggtgttcttg aacgtatccg caagctggca
     4141 ccacagcatg taaccgcgcc gtaccggacg gcagaagagt ggcgtgagtg gcaacttgcg
     4201 gaaggccaga aacgttgcga ggagatcaac cgtcagaatc gtcagttacg ggtggaaaaa
     4261 attctgaatc gctccggtat ccagccgttg caccgcaaat gttcgtttgc gaattactgg
     4321 gtgcagaacg acggccagcg atatgcgctg agccaggcaa aatccatcgc ggacgtactg
     4381 agaaccggat gcacaaattt cgtgttcagc ggtaagccgg gtaccggaaa aaatcacctg
     4441 gcagccgcca tcggtaatca tcttctggcg aaaggtcgca gcgtgattgt ggtcacggtg
     4501 gctgatgtga tgctggcgct acacggcatc tacgataaca aaaactcagg cgaaaaattt
     4561 ttgcagggtt tgtgtgacgt tgacctgctg gttctggatg agattggcat gcagcgtgat
     4621 acgcgcaacg aacaggtcac actgaaccag atcgtcgatc gcagaacagc cgcgatgcgc
     4681 agtgtcggga tgctgacgaa cctgaatcat accgcaatga gcaccctgct tggcgggcgc
     4741 gtgatggacc gcatgaccat gaacggtggg cgctgggtga attttaactg ggagagctgg
     4801 cgtgcgaatg tcggtcagcc aggaattgaa aagtaatttt atcggggaga aaaaatttaa
     4861 tggaaaccgt tttagacgca ctgaaagcaa tgggaaaagc cacgtcggta gaattggcta
     4921 cgcgacttga tatcagtcgt gaagaagttc tgaacgagct gtgggaactc aaaagaaatg
     4981 gcctcgttga taaaactggt cacatctggt ttctggctgg cgaaggtgaa tcccgggtaa
     5041 ccgaagagcg accagtaaaa tctgaagcac aggatatgct gaccggggag gttgaacaaa
     5101 aagttaccgc agacatgatg attgagttta tcagtcagga gggggctaaa acgtgtgagg
     5161 aactggcggg taagttcggt gtcagcactc gcaaggttgc ttccacactg gcggtagtaa
     5221 ccgcaacggg gcggctggca cgcgttaatc ggaacggtaa atttagttac tgcatgccgg
     5281 gcgataattt accggcagag ccgaaagctg caccggtaac gaaaacggct ggtaaggcct
     5341 ttcctcagcc agcaggtgtt gcgttaccag tacaggaagc tgcaacacag gaagaaatta
     5401 caacagatac tgtggcggac attgtgcagt cgctgccatc gtttactgaa acgcgagcgg
     5461 atgacctggt tttaccatcg ctgcatatgg caaaccgcga attgcgtcgg gcgaaaaatc
     5521 atgttcagaa gtgggagcga gtctgcgccg cgctgcggga gctgaacaag caccgggata
     5581 ttgttcgaca gattactgat tcttcccgcc gtgttgtatc ggaaaagtga ttgccggagg
     5641 cgcttatggc aaaagtattt acacaagaag agcgggaaaa attaaagggc aggttgtcga
     5701 actcgtgcga cagagcgggc gcgagacgtt acgacaactg gaagcgaaaa caggtgcgac
     5761 aagatatctg ctgagcgttc ttgccagaga gctggttgcc agtggtgatg tatacaattc
     5821 aggctacgga ttattcccgt ctgagcaggc gcgtaaagac tggcaaaacg cccgcaaaaa
     5881 actatcaagg gaaaaggtga aaaaaccggt tgtggttgat cctgacctta tctggtcatt
     5941 accagacgga gaaatacgcc gctacgacag gcgcctgaat ataatctgtc gcgagtgccg
     6001 gaagagcgaa gctatgcagc gtgtactggc attttattaa ggaaatgt
//
LOCUS       DBBGLQ010000187         6005 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.187, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000187 DBBGLQ010000000
VERSION     DBBGLQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.187"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..525
                     /gene="traF"
                     /locus_tag="ACIYSB_005069"
     CDS             <1..525
                     /gene="traF"
                     /locus_tag="ACIYSB_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HGC5431918.1"
                     /translation="LFNYNVLVKNYDSSAFRGDRYHNTKNGINADIGASMDLDDNWTL
                     GLVAQNLIPRSIETKEVNGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASSFTSD
                     SKRQFAAVGAEFNAWKWAQLRAGYRQNMASNDGSAFTAGIGLSPFDVVHLDIAGLAGT
                     DNTYGAVAQLSVTF"
     gene            596..805
                     /locus_tag="ACIYSB_005070"
     CDS             596..805
                     /locus_tag="ACIYSB_005070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431919.1"
                     /translation="MRNRKSTPAGLQLGFSPLSKTIMLARMRDIEGGRLRVGNDRGRD
                     FTNEAAQQLSQYRRVSTIISRQIWH"
     gene            963..1256
                     /locus_tag="ACIYSB_005071"
     CDS             963..1256
                     /locus_tag="ACIYSB_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431920.1"
                     /translation="MKTNKKDTKWYIFYRENSGEEILLEMSSFKECLSASKELMTPSN
                     YMICIERNGERIKRWDREIIAVSKKWINCPPDNFEILGELITIKKITKIDPQM"
     gene            complement(1306..2268)
                     /locus_tag="ACIYSB_005072"
     CDS             complement(1306..2268)
                     /locus_tag="ACIYSB_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGC5431921.1"
                     /translation="MAEWCRNRFEITGKSVCLDVLIQWIEGNDAPRYRHAIQQSILLF
                     LAGCAGILKPVRTVTYPPYPGLVSHGAGSTTVASQAFEQWLGLLARDAFLDHETVKTI
                     DRLYHQTGTGAIRWSNLPDTAKEIIGGVLAQQYADWFGVVDVYERPDNACLWESLLTF
                     AEYSQPCDMLQVMPTRLATELNGSGGLLKGTGTTRSFYCRQFGMEYPLGQNVRWKRTG
                     LSTLILEFDTIWCPPSGVLVGEISSVFDCEIRHWYSEPKNGINGYDCYDGGEHMDSKT
                     EAEWPQSDHAEEHEQTHLYLVRDSQPGERATASAESSGATAKIM"
     gene            complement(2376..2813)
                     /locus_tag="ACIYSB_005073"
     CDS             complement(2376..2813)
                     /locus_tag="ACIYSB_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HGC5431922.1"
                     /translation="MMNVKSVTEVDDAVVARVSEVLKFAFPGQKFEVLKVCDSGVYNM
                     INVYWLDGPTQAEVRFITRAFEGKNGLRFVHESREFSNEFVQECIDRLRQKYGQSNVP
                     PEVTVERYWKNDLWKIKTDRFPGNIEVAINEMGMETSKYRKVV"
     gene            complement(2889..3227)
                     /locus_tag="ACIYSB_005074"
     CDS             complement(2889..3227)
                     /locus_tag="ACIYSB_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431923.1"
                     /translation="MRPDLNTLPGDSGCSVWFYDGMSQPRLLAGSIAGLLTDVTITSN
                     YRGDVTSEIHDVVQEWLATGRCNLADLKEELWYYNLYINPSADEFMNANRRYGLGHTT
                     WLKGFINNAA"
     gene            complement(3295..3654)
                     /locus_tag="ACIYSB_005075"
     CDS             complement(3295..3654)
                     /locus_tag="ACIYSB_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431924.1"
                     /translation="MYSRFITGYWHLQTQSALLAHLCQLEGELTILRAWQRLGITPVP
                     EDEDEPSPLYSILWDVGEALGWLLYDLEMENVPAPGTIFHEVFDRVISLGYETEPGIW
                     AESISTGKRYAAMPDEF"
     gene            complement(3851..4360)
                     /locus_tag="ACIYSB_005076"
     CDS             complement(3851..4360)
                     /locus_tag="ACIYSB_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGC5431925.1"
                     /translation="MKTKTSAPAVYVGTWAKYNNGSIEGCWFNDLTVFETESDFLAAA
                     RKFHQDEKDPEFMYQDYENFPSGMASEYGFNWAYVEGYRLAREEGCEDAWYAWVSNTG
                     DTCFEQFRGAWLGGPYDSELEFAAQYVDECGFLSEVPDTVARYFDYEAFARDLFMELD
                     FVDGHVFHR"
     gene            complement(4501..4692)
                     /locus_tag="ACIYSB_005077"
     CDS             complement(4501..4692)
                     /locus_tag="ACIYSB_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431926.1"
                     /translation="MQISAETRAVLRHYRQIINARRREDGMKALSTPQIVDEICEFML
                     SQPAVYLGGQFIMQKARTR"
     gene            complement(4779..5165)
                     /locus_tag="ACIYSB_005078"
     CDS             complement(4779..5165)
                     /locus_tag="ACIYSB_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431927.1"
                     /translation="MGLDIFFLGRDRVCVTDAVVSVPETREVGYFRKVNPLIAWFEKH
                     CGPLENAVERPVSRTELEALLSDLECLTPENCREFFPTTEGFFFGSQEYDQYYWKDVE
                     DVKSWVRSTLDSFDFERKILLLWAWW"
     gene            complement(5318..5719)
                     /locus_tag="ACIYSB_005079"
     CDS             complement(5318..5719)
                     /locus_tag="ACIYSB_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431928.1"
                     /translation="MFIPLLFRAEMRLTPFFQRLRQRLCYPVSRVHTQHPWSSPQLRH
                     LYCTPWFVNATGNLALRACIVSIDGSLRHCFEFREGSHSREYRFWFEALPLSVLRFNT
                     FTGRISDRRTLSAASRMAAQLTSFRYRFGLR"
BASE COUNT         1693 a         1584 c         1368 g         1360 t
ORIGIN      
        1 ctgttcaact acaacgtgct ggtcaaaaat tatgacagca gcgcattcag gggagaccgc
       61 taccacaaca cgaaaaatgg catcaacgcc gacatcggtg ccagcatgga cctggatgac
      121 aactggacgc tgggtctggt cgcccagaac ctgatcccac gcagcattga gaccaaagaa
      181 gtaaatggtg tcactgaaac cttcaggatc cgtccgcagg taacgaccgg cgtatcatgg
      241 cacaacgaca tgttcaccac ggcactggat gtggatctga ccccggccag tagcttcaca
      301 tcagacagca aacgtcagtt tgccgccgtg ggtgctgagt ttaatgcctg gaaatgggca
      361 cagttacgtg ccggttaccg tcagaatatg gccagcaacg acggcagtgc attcactgcc
      421 ggtattggtc tctcgccgtt cgatgtggtg cacctggata ttgccggtct ggccggtacg
      481 gacaatacat acggggcagt ggcacaactg agcgtcacat tctgaacacc cagacagcat
      541 gccgcatacc gtcagcggca tgctggcaac tacactgaaa cagggacaat gactgatgag
      601 aaacaggaag agcacaccgg caggattgca gctgggtttt tcaccgttat caaaaaccat
      661 tatgcttgcc agaatgcgtg atattgaagg cggacgtctt cgcgtgggta atgaccgggg
      721 gagagatttt actaatgaag ccgcgcagca actttcacaa tacaggaggg taagcaccat
      781 catttcaaga caaatctggc actgaataag acgggcaata tagccgataa tgcgttgaat
      841 cattggctac atcgtatacg taaaacagac atcattgttc tgtctgttgt tcttacagaa
      901 taaattcctt tatttactgg aaatttataa ggtttttata gttatacgcc gagaataccc
      961 gtatgaaaac taataagaaa gacacaaaat ggtacatttt ttaccgtgaa aattcaggcg
     1021 aggaaatttt gcttgaaatg tcatcattta aggaatgtct ttcggcatcc aaagaattaa
     1081 tgaccccatc aaattacatg atatgcatag aaagaaatgg cgaaagaata aaacgttggg
     1141 acagggaaat catagcagtc tctaaaaaat ggattaactg cccacctgat aacttcgaaa
     1201 tattaggtga gctaataacc ataaaaaaaa taacaaaaat tgacccacaa atgtaataac
     1261 gccagtcctg aaaaaaagcg ccacagaatg tggcgcattg ttttattaca ttatttttgc
     1321 tgtcgcccct gaggactcag ctgatgctgt ggcacgttca cctggctgcg aatccctgac
     1381 cagatacaga tgagtctgtt cgtgctcctc tgcgtggtca gactgcggcc attcagcttc
     1441 cgttttgctg tccatatgtt caccaccatc ataacagtca taaccattga tcccgttctt
     1501 tggttcgctg taccagtggc gtatctcgca gtcaaacacc gatgaaattt cccccaccag
     1561 tacacctgaa ggcggacacc agatggtatc aaactccagt atcaacgtac tcagcccggt
     1621 tcgtttccac cggacattct gccccagtgg gtactccata ccaaactggc ggcagtaaaa
     1681 actgcgggtc gttccggtgc ctttcagcag accaccactt ccgttcagct ccgtggccag
     1741 ccgcgttggc atcacctgca gcatatcgca gggttgcgaa tactccgcaa atgttaacaa
     1801 cgactcccag agacaggcgt tatcagggcg ttcatacaca tccaccacgc caaaccagtc
     1861 agcatattgt tgtgcaagca caccaccgat tatctccttc gccgtatcag gcagattact
     1921 ccaccggatc gcgcctgtcc ctgtctgatg ataaagacga tctattgtct tcaccgtctc
     1981 gtgatcaagg aaggcatccc tggccagtaa tcccagccat tgttcaaacg cctggctggc
     2041 tacagttgtt gagccggcac cgtgactgac cagccctgga taaggcggat atgtcaccgt
     2101 tcttaccggt ttcagtatcc ccgcacagcc tgcaagaaac agcagtatgc tctgctggat
     2161 cgcatgacgg taccgggggg catcattccc ttcaatccac tgaatcagta cgtcaagaca
     2221 gactgactta ccggtgattt caaaacgatt tctgcaccat tcagccataa tcaatgctcc
     2281 tctggtaaat gaagagggca ttgtcccgtg aggaacaatg ccctcacggg aaaagcggaa
     2341 ttgtcacatt ccgccatcgt caaaagtcca gccggttata caaccttccg gtatttactg
     2401 gtttccatcc ccatttcgtt gatggcaacc tcaatattgc ccgggaaacg atctgtttta
     2461 atcttccaca gatcgttttt ccagtaacgc tccacggtca cctctggcgg aacgttgctc
     2521 tgcccgtact tctggcgaag ccggtcaatg cactcctgca caaattcatt actgaattcg
     2581 cgggattcgt gcacgaacct cagcccgttt ttgccttcaa acgcacgggt gataaaccgc
     2641 acttcagcct gtgtcgggcc gtccagccag tacacattaa tcatgttgta aacgccggaa
     2701 tcacacacct tcagcacttc aaatttctgc cccggaaatg cgaatttcag aacttcactg
     2761 acacgggcaa ccacagcgtc gtcaacctca gtcacagact taacgttcat cattctgctc
     2821 ctctctgtat gaaaatcact gagggaacag ccccgtgagg agctgagccc tcacgggatg
     2881 gcggtgtgtc acgccgcgtt attgataaag cctttcagcc aggtggtatg cccgaggcca
     2941 taccgcctgt tggcattcat aaattcgtct gccgacggat ttatgtagag gttgtagtac
     3001 cagagttctt cctttaaatc cgccaggtta cacctgccgg tcgcaagcca ctcctgcacc
     3061 acgtcatgaa tttcagatgt gacgtcacca cggtaattcg atgtgatcgt aacatccgtc
     3121 agtaacccgg caatactgcc cgccagcaga cgcggttgag acattccgtc atagaaccag
     3181 acgctgcagc cggaatctcc gggtaaagta ttcaaatcgg gtctcatgaa atttctcctg
     3241 aaaaagccgg gacccgtccc acagggatgg gtccctgtgg gaatgtggaa aacattaaaa
     3301 ttcgtcaggc attgcagcgt agcgctttcc ggtactgata ctttcagccc agataccggg
     3361 ctcagtctca taccccagag atataaccct gtcgaatacc tcgtggaaaa tcgttcccgg
     3421 tgcaggaaca ttttccatct ccaggtcata cagcaaccag cccagagcct caccaacatc
     3481 ccacaaaatt gagtaaagtg gtgaaggctc atcttcatcc tccggcaccg gggtaattcc
     3541 gagacgttgc caggcgcgaa gtatcgtcag ttcaccttca agctgacaga gatgtgccag
     3601 caaagcggac tgcgtctgaa gatgccagta accagtaata aaacgcgaat acataaaccc
     3661 ccctgtctga ttctttctga tgaaagagca caccgtccga aggaacgatg cctccgggcg
     3721 gtgaacaaca gaattcaggt tgttcatccg cgatgattaa aattgtcaac aacggtaaaa
     3781 aaaccagcag aatgtatgct gaaatcacat ccgccagtac acggcttata tgcgccctca
     3841 tggctgtgag ttaccgatgg aaaacgtgtc cgtcgacaaa atccagttcc atgaacaaat
     3901 cgcgcgcaaa agcctcataa tcaaaatagc gtgcaacagt atcaggtacc tctgacagaa
     3961 aaccgcattc atccacgtac tgcgcggcga actcaagttc actgtcataa ggaccaccaa
     4021 gccacgcccc ccggaactgt tcgaaacagg tatcaccggt attagaaacc catgcatacc
     4081 atgcatcttc acagccttct tcacgggcaa gacgataacc ttcaacataa gcccagttga
     4141 aaccatattc tgaagccatt cccgaaggaa agttttcata atcctggtac ataaattcag
     4201 gatctttttc atcctgatga aacttacggg ccgcagctaa aaaatcgctt tcagtttcaa
     4261 aaacggttaa atcattaaac cagcatcctt ctatgctgcc attgttgtat ttagcccagg
     4321 taccaacata tacagcaggt gcagaagttt tcgttttcat gagagtgtcc ttaatgaaaa
     4381 gggttcactc ccaccgtacg gtatgagagt gaaccgtacg gttaacagta aaatcagacc
     4441 cgcctcacca tgtggtggcg aaagcataac ggtctgttga aaagccatga acaaagacgg
     4501 ctatcttgtt ctggccttct gcatgatgaa ctgcccaccc agatacactg ctggctggct
     4561 gagcataaac tcacagatct catcaacgat ttgcggtgtg ctgagagctt tcatgccatc
     4621 ttcacgccgt ctggcgttaa tgatctgtct gtagtgacgt aaaacagcgc gagtttctgc
     4681 acttatttgc ataatctcat tctccggaat aaaagcaggc cagagaagtc gggcttctgc
     4741 gaataatcac atggttaaaa acaaggactg agaaatagtt accaccatgc ccagagcaac
     4801 agtatttttc gttcaaaatc aaacgaatca agcgtgcttc tgacccacga cttcacgtcc
     4861 tcaacatcct tccagtaata ctggtcatac tcctgagagc caaaaaagaa tccttctgtt
     4921 gtggggaaga attcccggca gttctccggc gtaaggcatt caagatcgct cagtagtgcc
     4981 tccagttccg ttctggaaac cggacgctcc acggcatttt caagcggtcc acaatgcttc
     5041 tcaaaccagg caatcagtgg gttaaccttg cgaaagtacc ccacttcccg cgtttccggt
     5101 accgatacca ctgcgtcggt aacacaaaca cgatcacgcc caaggaaaaa aatatcgagt
     5161 cccataaaca atctccagaa acagagagac cgttccccga acggaagcgg tcccggacgg
     5221 gttaaggaaa aatcagcttt gtcatcgcgt tctgaatggc gcgatcagac aacgctgctc
     5281 gtagagaaaa tgactgtcat aacgacctca aacagcttca gcgcaaaccg aagcgatagc
     5341 gaaaggaagt aagctgcgcg gccatacggg aggcagcaga gagtgtacgg cgatcggata
     5401 ttcgaccggt aaaagtgtta aaacgcagta ccgacaaagg aagcgcctca aaccagaaac
     5461 ggtattcacg gctgtgagag ccttcacgga actcaaagca gtgtcgcaaa gaaccgtcaa
     5521 tggaaacaat gcaggcacgg agtgcaagat tgccggtggc gttaacaaac cacggcgtgc
     5581 agtaaaggtg ccgcaactgt ggagaagacc agggatgttg tgtatgaaca cgactcaccg
     5641 gataacacag acgttgtctg agtcgctgaa agaaaggtgt caggcgcatc tctgcgcgaa
     5701 acagaagtgg aataaacatc ggaacctccg gataaaagac ggacgttccg ccctgacgga
     5761 ctggaaaatc cgtcaggtga acacatcaat gcgataacac gatgtgctta aggaccagag
     5821 ataactcagg tcatacagtg ataaaaaagc cttcatcatc acggaaaacc gcagtgacat
     5881 ctgctctcaa aggtgtgcag atttgcccgt acttccggaa acatatccct tcagtacgca
     5941 cagctccttt caggctcagg aggtgtaaaa ctgaccaccc gaaggcgctc ctcacaggtc
     6001 agatg
//
LOCUS       DBBGLQ010000188         5894 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.188, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000188 DBBGLQ010000000
VERSION     DBBGLQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.188"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            57..1210
                     /gene="yiaY"
                     /locus_tag="ACIYSB_005080"
                     /pseudo
     CDS             57..1210
                     /gene="yiaY"
                     /locus_tag="ACIYSB_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
     gene            1375..2913
                     /gene="aldB"
                     /locus_tag="ACIYSB_005081"
     CDS             1375..2913
                     /gene="aldB"
                     /locus_tag="ACIYSB_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGC5431929.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGVIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3132..3275)
                     /locus_tag="ACIYSB_005082"
     CDS             complement(3132..3275)
                     /locus_tag="ACIYSB_005082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431930.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3458..3781
                     /locus_tag="ACIYSB_005083"
     CDS             3458..3781
                     /locus_tag="ACIYSB_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGC5431931.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3787..4923
                     /locus_tag="ACIYSB_005084"
     CDS             3787..4923
                     /locus_tag="ACIYSB_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGC5431932.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4920..>5894)
                     /locus_tag="ACIYSB_005085"
     CDS             complement(4920..>5894)
                     /locus_tag="ACIYSB_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5431933.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1455 a         1403 c         1545 g         1491 t
ORIGIN      
        1 cgaatgaaaa tcctcagtaa gctgcccgcc cttttttaca ctttcaggag tgtgttatgg
       61 cagcttcaac gttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg
      121 caatgaatat gatggcagat tatggattta cccgtacctt aattgtcact gacaatatgt
      181 taacgaaatt aggtatggcg ggcgatgtgc aaaaagcact ggaagaacgc aatattttta
      241 gcgttattta tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga
      301 aattacttaa agagaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg
      361 actgcgcaaa aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag
      421 gcgttgaccg ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc acggcgggta
      481 cggcctctga aatgacccgt ttctgcatca tcactgacga agcgcgtcat atcaaaatgg
      541 cgattgttga taaacatgtc actccgctgc tttctgtcaa tgactcctct ctgatgattg
      601 gtatgccgaa gtcactgacc gccgcaacgg gtatggatgc cttaacgcac gctatcgaag
      661 catatgtttc tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca
      721 tgattgccga aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag
      781 caatggctta tgcccagttc ctcgccggta tggcgttcaa taatgcttct ctgggttatg
      841 ttcatgcgat ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg
      901 ccgttttgct gccgcacgtt caggtattca acagcaaagt cgccgctgca cgtctgcgtg
      961 actgtgccgc tgcaatgggc gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag
     1021 cctgcattaa cgccatccgt gaactggcga agaaagtgga tatcccggca ggcgcctacg
     1081 cgacctgaac gtgaaagaag aagatttcgc ggtattggcg actaatgccc tgaaagatgc
     1141 ctgtggcttt actaacccga tccaggcaac tcacgaagaa attgtggcga tttatcgcgc
     1201 agcgatgtaa tcatcatttc cacaacggct ggcaaattgt cagccgcttt ttcaactatc
     1261 tctgtaaccc ttgcccgtaa attcgtgata gctgtcgtaa agctgttacc gactggcgaa
     1321 gatttcgcca gtcacgtcta cccttgttat acctcacacc gcaaggagac gatcatgacc
     1381 aataatcccc cttcagcaca gattaagccc ggcgagtatg gtttccccct caagttaaaa
     1441 gcccgctatg acaactttat tggcggcgaa tgggtagccc ctgccgacgg cgagtattac
     1501 cagaatctga cgccggtgac cgggcagctg ctgtgcgaag tggcgtcttc gggcaaacga
     1561 gacatcgatc tggcgctgga tgctgcgcac aaagtgaaag ataaatgggc gcacacctcg
     1621 gtgcaggatc gtgcggcgat tctgtttaag attgccgatc gaatggaaca aaacctcgag
     1681 ctgttagcga cagctgaaac ctgggataac ggcaaaccca ttcgcgaaac cagtgctgcg
     1741 gatgtaccgc tggcgattga ccatttccgc tatttcgcct cgtgtattcg ggcgcaggaa
     1801 ggtgggatca gtgaagttga tagcgaaacc gtggcctatc atttccatga accgttaggc
     1861 gtggtggggc agattatccc gtggaacttc ccgctgctga tggcgagctg gaaaatggct
     1921 cccgcgctgg cggcgggcaa ctgtgtggtg ctgaaacccg cacgtcttac cccgctttct
     1981 gtactgctgc taatggaaat tgtcggtgat ttactgccgc cgggcgtggt gaacgtggtc
     2041 aatggcgcag gtggggtaat tggcgaatat ctggcgacct cgaaacgcat cgccaaagtg
     2101 gcgtttaccg gctcaacgga agtgggccaa caaattatgc aatacgcaac gcaaaacatt
     2161 attccggtga cgctggagtt gggcggtaag tcgccaaata tcttctttgc tgatgtgatg
     2221 gatgaagaag atgccttttt cgataaagcg ctggaaggct ttgcactgtt tgcctttaac
     2281 cagggcgaag tttgcacctg tccgagtcgt gctttagtgc aggaatctat ctacgaacgc
     2341 tttatggaac gcgccatccg ccgtgtcgaa agcattcgta gcggtaaccc gctcgacagc
     2401 gtgacgcaaa tgggcgcgca ggtttctcac gggcaactgg aaaccatcct caactacatt
     2461 gatatcggta aaaaagaggg cgctgacgtg ctcacaggcg ggcggcgcaa gctgctggaa
     2521 ggtgaactga aagacggcta ctacctcgaa ccgacgattc tgtttggtca gaacaatatg
     2581 cgggtgttcc aggaggagat ttttggcccg gtgctggcgg tgaccacctt caaaacgatg
     2641 gaagaagcgc tggagctggc gaacgatacg caatatggcc tgggcgcggg cgtctggagc
     2701 cgcaacggta atctggccta taagatgggg cgcggcatac aggctgggcg cgtgtggacc
     2761 aactgttatc acgcttaccc ggcacatgcg gcgtttggtg gctacaaaca atcaggtatc
     2821 ggtcgcgaaa cccacaagat gatgctggag cattaccagc aaaccaagtg cctgctggtg
     2881 agctactcgg ataaaccgtt ggggctgttc tgatataaga agctggtcgc attgggtatt
     2941 cattgcctga tgcgacgctt acgcgtctta tcatgcctac gggaacctga ccgtaggccg
     3001 gataaggcgt acacgccgca tccggcataa acaaagcgca cgttgttaca atttacgttt
     3061 taaacctacg tgcgcaataa cccagactac gccaacagaa gtacgtttct ggggcgtgtt
     3121 ttgtcaggag tttaatctcg cgtttcgatg gtgagcgggc gaaccaggcc gtaaatcacc
     3181 attggtgatc cgaggccaat cagcaataaa cggtagataa ttgaaggttt aatacgctta
     3241 atctgtactg tggatgtttt actggagtta ttcattagtt tggccaatag ttccactatt
     3301 tcaatatgat aaacctcgtc ctgttgaaaa accagacagc acatttaaga aagtatataa
     3361 aaaacaatat gtcgttatat aaataaggaa attgcactgc atcatggcat ttcataatat
     3421 gcgttaattg ttctttttta tattctttga gggaaatatg ttcctggact attttgcact
     3481 gggagtgctt atttttgtat ttctggtgat cttctatggg atcattattt tacatgatat
     3541 tccctacctg attgccaaaa aacgtaatca tcctcatgcc gacgccattc atgttgctgg
     3601 ttgggtgagt ctttttacgt tacatgttat ctggccgttt ttgtggattt gggccacgct
     3661 ttaccgcccg gagcggggat ggggaatgca aagccatgat tcatccgtta tgcaactgca
     3721 acagcgcatt gccgggctgg agaaacagct cgccgacatc aaatcctctt ctgccgagta
     3781 atacttatgg atttactgat tattttgacc tatgtggctt ttgcatgggc aatgtttaag
     3841 atcttcaaaa ttcccgtaaa taaatggacc attcccacag cggccctggg tggaatattt
     3901 attgtcagcg gtctaattct gttaatgaac tataaccatc cgtatacctt taaagcgcaa
     3961 aaagcggtta tttctattcc tgttgtccca caggtgactg gtgtggtgat tgaagtgacg
     4021 gataagaaaa atacgctgat taaaaaaggt gaggtgctat ttcgactgga cccgacgcgt
     4081 tatcaggcgc gggtggatcg gctgatggcg gatatcgtta ccgcagaaca taaacagcgg
     4141 gcgttgggcg cagagttaga tgagatggcg gcgaatactc agcaggcaaa ggccacgcgg
     4201 gataaattcg ctaaagagta tcagcgttac gcacgtggca gccaggcgaa agtaaacccg
     4261 ttttcagaac gcgatatcga tgtggcgcgg caaaattatc tggcgcagga agcctccgtg
     4321 aagtcatcgg cggcggaaca aaaacagatc cagagccagc tggatagcct ggtgttgggt
     4381 gaacattctc aaatcgccag cctgaaagca cagctcgcgg aagcaaaata taaccttgag
     4441 cagacgatag tgcgtgcgcc gagcgatggt tatgtgaccc aggtactgat tcgtccgggt
     4501 acctatgccg cgtcgctgcc gctacgtccg gtgatggtgt ttatacccga tcagaaacga
     4561 caaatcgtgg cgcagttccg tcagaactcc ttgctgcgac tggctcctgg cgacgatgcg
     4621 gaagtggtat ttaatgctct gccaggtaag gtattcagcg gtaagctggc agccattagt
     4681 ccagccgttc ccggcggagc ttatcagtcg accggcacct tacagacgtt aaacacagcg
     4741 ccgggttcag atggcgttat cgcgaccatt gaactggatg agcacactga tttgagcgct
     4801 ttaccagacg gtatttacgc ccaggtggcg gtttactctg atcatttcag ccatgtctcg
     4861 gtgatgcgca aagtgctatt acgcatgacc agctgggtgc attaccttta tctcgatcat
     4921 taatcttcgc tttccgccgc ttgttcaggc cgcagttgct gcgactgagc aagctggtgg
     4981 cgaatttcac gcagcatttc atccagcaca tactggaccc gccagggctg gtattcccgt
     5041 ttacggaaga tagccaccag atccagccac cattcatagg gttgctgaaa gcagggcacc
     5101 agcgtgccgt cttgcaggtc gctctgcaca cttttgtctg gcgcaaacac aatccccagg
     5161 tgatttcgtg ccagctccag cgcagattgc gtgttgtcgc aaacgtaatt tcccttcacg
     5221 cgataatccc gcacctcttt actcccggcg acgttgaagc gccagatatt cgcgtcgtcg
     5281 atcatcatcg agtcgatcaa aatacaggag tgatgttcga gttcgtcagg acggctaatg
     5341 ggatgtttct caagatagcg ctggctggca taagcggtta cggcatattg ggtaataaaa
     5401 ctggcaacca gcgattcatc ttttggtgga gcgtagctga ttaaaacatc acaatcatca
     5461 ggaaattcga cgccttcata aaaggcatta cgctcaagat tgcaggtttt tagcgataag
     5521 gtgatatcac cgatatcttt aattttatcg attacatgtt tggataaata ggtaattatg
     5581 ccggttgggg cgtagatggt gacccgacca cgtttctcat gcttataatc tgcaataaaa
     5641 ttattaagtt gctcgtttct gtccagcatg tcgttgatgt acggtaacag cgcggtgcca
     5701 aaaggtgtga gcatcagctg ccgggtggtt cggtcaaaga cttttaaacc cacttttgat
     5761 tcaaaatcag caagatattt gctgacgttg gcctgtgcga tgccaagtac agtcgcggca
     5821 tggctgatat tttcactggc agcgattacc gagataattt ttaactcccg gtacttaagt
     5881 tgtaattttg tcat
//
LOCUS       DBBGLQ010000189         5843 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.189, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000189 DBBGLQ010000000
VERSION     DBBGLQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.189"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            497..724
                     /locus_tag="ACIYSB_005086"
     CDS             497..724
                     /locus_tag="ACIYSB_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HGC5431934.1"
                     /translation="MANRKQQRAYAARRHIQTEINRRLSRAFRVAHIMHINMLHERSH
                     ALSNMYSAAVFSYLADDLRKLQDLINQHYHH"
     gene            793..1206
                     /locus_tag="ACIYSB_005087"
     CDS             793..1206
                     /locus_tag="ACIYSB_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431935.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDLHDYYRRACKQAAS
                     SATTYGNFSFTVDDRYRVDVRDQNHSMVLGLFHINGCDSSVISWSSSEMVLVLEMTTH
                     SVVVKGIKTAEDFIAEYQRLLVLYRAAFEKATAGE"
     gene            1212..3722
                     /locus_tag="ACIYSB_005088"
     CDS             1212..3722
                     /locus_tag="ACIYSB_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGC5431936.1"
                     /translation="MSHHHTHLAYPWNAPLPVIDPETFGRALPQKPTALQRAIRDYLT
                     EDIRTEARLDEETVAFLTTTDKGKQINSHLHRDEERQIRLDKLARHRRENPPTAVSEA
                     IAELRTLPSFLASVLIRDLARLNRKQQSARSEGVKNNSAYVADNFVRRGLRARLKRIE
                     RVNDRFATPAFKTTVVRHRLEELLTLPQLTRDEIQRIATLTAQAFAAEFERICDEVLE
                     RTGKDNDSPVIWLLAYQKVARTALILGVVPPYWPELEIRHNRRTPPDPERVPGAVLRI
                     TCATWWNNQLRHVADLWREELLRAAGRVSRKQSPYISHESLLEFREKRQRTRDFLKSW
                     DIENEDGERLSLEDVYWAGLGNPRNRRNEMMVCVRGMEMVAELRADVAFFITVTTPSR
                     FHSVNENGSLNPKYNGATVRDASDYLVYDFFAAVRKKIKKEGLGWYGVRTAEPHHDGT
                     PHWHMLVFTSPENAERIIEIMREAAIKSDRHELGDDISPRFKCEKIDPEKGTPASYIA
                     TYIGKNLDASAFHNNDPKTGKPYVDEESGKTMAETVENAIAWASLHRIRQFQFFGIPP
                     RQVWRELRRLASQMERNPASPKHLDHDDIDAIMAAADVGCFATYIMKQGGVLIPRNQY
                     LVRTAYETADEANDYGEFPQRIYGICAPSLGERYTICTHPDEWKLVKKETTPDNRTGE
                     GFDLQGDPVAPWTRGNNCPRDEKTDNHGPENRQDPGRLTLPEENLESWLNTLSRGDRR
                     KLRNQIKNQPSGAKTGQNDTTPASDTPVRVGIVVPADTATDTQIARELDALGVQLPDA
                     AIISVRNGARVRLDNGGTAYWDESARHIITEQPVEAGA"
     gene            3719..4150
                     /locus_tag="ACIYSB_005089"
     CDS             3719..4150
                     /locus_tag="ACIYSB_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431937.1"
                     /translation="MTRFCCTMQRPQFEAIMRQRMPVLTLFRPATRPQPGDELEITIV
                     SPDRTTETTIADILNRRPGSESHSWTVVIRPRRSPEEPEPCTPAGKIMQRYHEERTRR
                     HNGGLARTLNTVNHKAALARMVFSPQRNSFARPAQNGRKQQ"
     gene            4147..4341
                     /locus_tag="ACIYSB_005090"
     CDS             4147..4341
                     /locus_tag="ACIYSB_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431938.1"
                     /translation="MSRILLFLLFFAPFAQSATPNCVAKKSNTVVIVQCDDGTVTITD
                     SSKGSVIVCRKEKPCQRTEL"
     gene            4353..4667
                     /locus_tag="ACIYSB_005091"
     CDS             4353..4667
                     /locus_tag="ACIYSB_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431939.1"
                     /translation="MNRKQKQELKGFLRKEIARLEDVESQSSDIPFGMDINDACMLQV
                     YRIAQAALQAKPSDGLVRAVRFYELVKRENPPVETGAWQDAVDWVLKEACQVVNTGIK
                     GD"
     gene            4669..4878
                     /locus_tag="ACIYSB_005092"
     CDS             4669..4878
                     /locus_tag="ACIYSB_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431940.1"
                     /translation="MAIAASYTMHLYCDCRQCTNGKYQTPDFGEYIGTSWTGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            4875..5009
                     /locus_tag="ACIYSB_005093"
                     /pseudo
     CDS             4875..5009
                     /locus_tag="ACIYSB_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5560..>5843
                     /locus_tag="ACIYSB_005094"
     CDS             5560..>5843
                     /locus_tag="ACIYSB_005094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431941.1"
                     /translation="MGVKDKAVKIALRQREYVVRVAGLRKHPPGDDGYKVGDVVKLSE
                     GITSLQENPGKVRIAAGINSKEDNVMSHFNDLCQINREAEEKRAEAAQILR"
BASE COUNT         1571 a         1589 c         1560 g         1123 t
ORIGIN      
        1 acctggtgga gttggtaact gaactgcgcg atgaacaggg gatgacattc agcgaaatca
       61 gaaagtacat ccgggacacg accgggatat cccttaaaag atgggtcctc gcccggcttt
      121 acgcacgata taccgccgct gacaccgtgc tttgcgaact gctaccggac tgagtaacca
      181 ctatgcacac acaaaaaaac cgcttgccat gccgcaatca gtcaggttac atttccgctg
      241 cacctcataa aacgggtgcc gggattgcaa cccccgctga caaacgaagc gcacaaccgc
      301 gccagcggtt tttttgtgcg taccgtatcg ccacgtcttt ttcgcaccag aattatggtg
      361 gggcgtacgg ggccgacttc ggtcgggccg gtttcttcgt ttgccggtgg ttgcaaccct
      421 gtacgtctcg ccacccagaa ttgcaacctc tggatggtga gtatccaaaa actcacaaac
      481 gaagaggcca aacaccatgg caaaccgcaa acaacagcgc gcatacgctg cgcgtcgtca
      541 catccagact gaaatcaacc gcagactttc ccgcgcattc cgcgtcgcgc atatcatgca
      601 catcaatatg ctgcatgagc gcagccacgc actgtcaaac atgtattccg ccgctgtatt
      661 cagctacctg gcggatgacc tgcgcaagct gcaggacctc attaaccagc actaccacca
      721 ctaatcaaca tcccggagcc atcccgcccc cgtacgcggg aggcttccgc acgtccgttt
      781 ccggaggttc tcatgaagag cgttaaggga ctttcagata ttcaggattt ttttaactgg
      841 atgtgggcgg tggatccacg cctcgcttcc cgtctcaatg acctgcatga ctattaccgc
      901 cgcgcctgta aacaggccgc cagcagcgcc accacatacg ggaatttttc cttcaccgtt
      961 gatgaccgtt accgcgtgga tgtcagagac cagaaccaca gcatggtgct gggcctgttc
     1021 cacatcaacg ggtgcgacag cagcgttatc agctggtcat cgtcagaaat ggtgctcgtt
     1081 ctggaaatga ccacccacag cgtggtcgtg aaagggatta aaacagcaga agactttatc
     1141 gctgaatacc agcgccttct tgtgctgtac cgggctgcat ttgagaaggc aacagcaggg
     1201 gagtaagcgg aatgagccat caccacacgc acctggcgta cccgtggaac gccccgctgc
     1261 cggttattga tcctgagaca ttcggcagag cattaccgca gaagccaacc gcattacaac
     1321 gggcaatccg tgactacctc accgaagaca ttcgcactga ggcccggctg gatgaagaaa
     1381 ccgtggcctt cctgacaacc acggacaaag gtaagcaaat taattcacac cttcaccggg
     1441 acgaagagcg gcagataagg ctggataagc tggcacgaca cagacgcgaa aatccgccaa
     1501 cggcggtatc ggaggcgata gccgaacttc gcaccctgcc ctcgtttctt gccagtgtgt
     1561 tgatccgtga tctggcacga ctgaaccgga aacagcaaag tgcccgcagt gaaggtgtaa
     1621 aaaacaacag cgcatacgtg gccgataatt tcgttcgccg tggactgcgt gcgcgcctga
     1681 aacgtattga aagggttaac gaccgctttg caacgccggc attcaaaacc accgtcgtgc
     1741 gccatcgcct tgaagaactg ctcacactgc cgcaactcac ccgtgacgaa atccagcgca
     1801 tcgccacact gacagcccag gcattcgccg cagaatttga acgcatatgt gatgaagttc
     1861 ttgaacggac aggcaaggat aatgacagcc cggttatctg gctgctggcc taccagaagg
     1921 tggcacgcac agccttaatt ctgggcgtgg tcccgccata ctggccggaa ctcgaaatca
     1981 ggcacaaccg acgcacaccg ccggaccctg aacgggttcc gggtgcggta ctgcgcatca
     2041 cctgcgcgac ctggtggaac aaccagttac gccacgtcgc cgatctctgg cgggaggagt
     2101 tactgcgcgc cgccggcaga gtgtcacgta aacaatcccc ctatatcagc catgagtcac
     2161 tactggagtt ccgcgagaag cgccagcgca cccgtgactt tctcaaaagc tgggatattg
     2221 aaaacgaaga cggcgagcgc ctgagtctgg aggacgtgta ctgggctggc ctgggtaatc
     2281 cacgcaaccg ccgcaatgaa atgatggtct gcgtcagagg gatggagatg gtggccgaat
     2341 tacgggctga cgtcgcattc ttcatcacag taaccacccc ttcccgcttt cacagcgtga
     2401 acgagaacgg cagcctgaac ccgaaataca acggtgccac agtacgagat gccagtgatt
     2461 atctggttta cgacttcttc gcagccgtaa gaaaaaaaat aaaaaaagag gggctgggct
     2521 ggtacggcgt gcgaaccgct gagccacacc atgacggcac gccacactgg catatgctgg
     2581 tattcacttc cccggagaac gcagagcgca tcatcgaaat tatgcgcgaa gcagcaatta
     2641 aatcagatcg ccacgaactg ggggacgata tttcaccgcg ctttaaatgc gagaagatag
     2701 acccggaaaa aggcacaccg gcaagctata tcgcaaccta tatcggtaag aacctggacg
     2761 ccagcgcttt ccacaacaat gatccgaaaa ccggaaaacc ttacgttgat gaggaatccg
     2821 ggaaaaccat ggcggaaacc gtggagaacg ccattgcctg ggcaagcctt caccgcatcc
     2881 gtcagttcca gtttttcggc attccgcccc gccaggtatg gcgtgaactc cgccgccttg
     2941 caagccagat ggagcgcaac ccggcatcac cgaagcacct cgatcatgac gatattgacg
     3001 ccattatggc tgccgcagat gtgggatgtt ttgccaccta catcatgaaa cagggtggtg
     3061 tgctcattcc gcgcaaccag tatctggtcc gcaccgcata cgaaaccgca gacgaagcca
     3121 acgactacgg tgaattccct caacgtatct acggaatctg cgccccgtcg ctcggagagc
     3181 ggtacaccat atgcacccat ccggacgagt ggaaactggt taaaaaagaa acaacaccag
     3241 acaaccgcac gggcgagggt tttgaccttc agggcgaccc cgtcgcccct tggactcgtg
     3301 gcaataactg tccccgggat gaaaaaacag acaatcacgg accagaaaac aggcaggacc
     3361 ccggacgact gacgcttccc gaagagaacc tggaaagctg gctgaatacg ttaagcaggg
     3421 gcgatcggag aaaactgcgt aatcagatta aaaatcagcc gtcgggcgca aaaaccggac
     3481 aaaacgacac cacaccggca tctgacaccc ctgtaagggt ggggattgtc gtgcctgcgg
     3541 ataccgcgac ggacacgcaa atcgcccgcg aacttgatgc gctgggtgtg cagttgcctg
     3601 acgcggccat catctccgtg cgtaacggtg cccgtgtgcg tctggataac gggggaacag
     3661 cgtactggga tgagtcagcc cgtcacatca tcacagagca gccagtggag gcgggagcat
     3721 gacgcgattt tgctgcacca tgcagcgccc acagtttgag gccatcatgc gccagcgtat
     3781 gccggtgctg acactgttca ggcctgccac aaggccacaa ccgggcgacg agctggaaat
     3841 cacgatagtc agccccgatc gcaccacgga gaccaccatc gccgacatac tcaacaggcg
     3901 gccgggcagc gagagccata gctggactgt cgtcattcgt ccgcgccgtt cgccggagga
     3961 accggagccg tgcacgcctg ccgggaaaat catgcagaga tatcacgaag agcggacacg
     4021 caggcacaac ggcggactgg ccagaacact gaacacagtc aatcacaagg ccgcgctggc
     4081 gcgaatggtc ttttcaccac aacgcaattc attcgccaga ccagcacaga acgggaggaa
     4141 acaacagtga gcagaatatt actttttctg ctgtttttcg cgccattcgc acaatcagcc
     4201 acgccgaact gcgtagcgaa aaagagcaac accgttgtca ttgtccagtg tgatgacggc
     4261 acggtaacaa tcaccgactc gagcaaagga agcgtcattg tctgccgtaa agagaaaccc
     4321 tgtcagcgaa ccgaactttg aaggaaaaat atatgaacag gaaacagaag caggaactga
     4381 aaggcttctt acgtaaagaa attgccaggc tggaagatgt ggaatctcag tcatcagata
     4441 ttccattcgg aatggatatc aacgacgcct gtatgctcca ggtataccgc atagctcagg
     4501 ctgcactaca ggcaaaaccg tcagatggac tggttagagc agtacgtttc tatgaactgg
     4561 taaagcgtga gaatccgcca gtcgaaaccg gagcatggca agacgctgtt gactgggtgc
     4621 tcaaagaggc ttgtcaggtt gtaaacactg gcatcaaagg agactgatat ggctattgcc
     4681 gcaagttaca ccatgcatct ctattgtgat tgtcgccagt gtacaaatgg taaatatcaa
     4741 acgccagact tcggtgaata tatcggtacg tcatggactg gctgtgcaaa agaggcgcgt
     4801 aaggatggct ggcgaataag caaagacaaa actcgtgctt ttgcgcccgg gcataaagtt
     4861 ttgaggatta acaaatgacc actataacca aagagcgact actgacaatc aggcagtggc
     4921 gcgaaacata cggacctggt agcaacgttg tactgccagc cgaagaagcg gaaaaactgg
     4981 cacggattgc tttggcatcg ctggaagcaa aaccaatagg tgctttccac attgcagaac
     5041 agcaggtcga tggcacaagt gactacatca aggatgggga gtaccgcttg ctgacatctg
     5101 aagaacaaaa gatagcccaa cttttaggcg atgcatggaa cctgtatttt acattgccag
     5161 ttgagcatcc aatgggtaga gatgaatttt gccgggcaat tcatcattgc caaaatatgg
     5221 tgttggcacg ccctgccatc agggcgctgg ccagtaaggg gcaaggctat aaatagatga
     5281 gcaaaacgcc ctcctccagg gcgtttattt caatgcacaa tagtgcacaa atttgcacaa
     5341 tttttttgaa cgactttttg cccttccggc ccgtgtggcg tctggatccg tcggggatcc
     5401 gtgcgtgcac gaaaaaaggc ctttttctgc gcgcaggtga cgggggaaca gcccgcgttt
     5461 cagggggtaa atagcattcc ctgaacgatg ttgcagcgac acaacagaat ggctgtattt
     5521 ctcacgctga gcgtaaaaaa gacgtaaaag cttgatttga tgggggtgaa agataaggcc
     5581 gtcaaaatcg cactgaggca gcgagaatat gtcgtcagag ttgcggggtt gcgtaagcat
     5641 ccgcccggcg atgatggcta caaggttgga gatgttgtga aattatctga aggaataacc
     5701 agcttgcagg agaacccggg caaagtcaga atagcggcag gcatcaactc aaaagaggat
     5761 aatgtcatga gccactttaa tgatttatgc cagatcaatc gtgaagctga agaaaaacgc
     5821 gctgaggcag ctcaaatttt gcg
//
LOCUS       DBBGLQ010000190         5759 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.190, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000190 DBBGLQ010000000
VERSION     DBBGLQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.190"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            274..1206
                     /gene="rfaD"
                     /locus_tag="ACIYSB_005095"
     CDS             274..1206
                     /gene="rfaD"
                     /locus_tag="ACIYSB_005095"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGC5431942.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1216..2262
                     /gene="rfaF"
                     /locus_tag="ACIYSB_005096"
     CDS             1216..2262
                     /gene="rfaF"
                     /locus_tag="ACIYSB_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGC5431943.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2266..3258
                     /gene="rfaC"
                     /locus_tag="ACIYSB_005097"
     CDS             2266..3258
                     /gene="rfaC"
                     /locus_tag="ACIYSB_005097"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGC5431944.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSRIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3255..4463
                     /gene="rfaL"
                     /locus_tag="ACIYSB_005098"
     CDS             3255..4463
                     /gene="rfaL"
                     /locus_tag="ACIYSB_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGC5431945.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4499..5641)
                     /locus_tag="ACIYSB_005099"
     CDS             complement(4499..5641)
                     /locus_tag="ACIYSB_005099"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HGC5431946.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(5650..>5759)
                     /locus_tag="ACIYSB_005100"
     CDS             complement(5650..>5759)
                     /locus_tag="ACIYSB_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HGC5431947.1"
                     /translation="AVKKHEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         1592 a         1249 c         1286 g         1632 t
ORIGIN      
        1 attatatcat aaatattaat agaatgtata ctgtatatta tccattcaaa tgcttccgtt
       61 atttatcata catcggaata ttgatactaa agcactattc acatgcaaaa ccaacatccg
      121 ccatgaagga ctagctaaaa cccaaactag tttgttgcaa ttagcatcct tgcacctcta
      181 tgtaaagggc tgaagggatt cggatgtgat ggtatgatta cagacattcg tgtctgagat
      241 tgtctctgac tccataattc gaaggttaca gttatgatca tcgttaccgg cggcgcgggc
      301 tttatcggca gcaacatcgt taaagccctg aatgataaag gcatcaccga tattctggtg
      361 gtggacaacc tgaaagacgg caccaagttt gtgaacctgg tggatctgaa tatcgcagac
      421 tatatggata aggaagactt cctgatccag attatggctg gcgaagagtt cggcgatgtc
      481 gaagcgattt tccacgaagg cgcgtgctct tccaccaccg agtgggacgg caagtatatg
      541 atggataaca actatcaata ctccaaggag ctgctgcact actgcctgga gcgtgaaatc
      601 ccgttcctgt acgcttcttc cgcagccacc tacggcggac gcacctccga ctttattgaa
      661 tcccgcgagt acgaaaaacc gttgaacgtc tacggttact caaaattcct gtttgatgaa
      721 tatgttcgtc aaatcctgcc tgaagcgaac tcgcagattg ttggcttccg ttatttcaac
      781 gtttatggac cgcgtgaagg ccataaaggc agcatggcga gcgtcgcttt ccatctcaac
      841 acccagctta acaacggtga atcaccgaag ctgtttgaag gtagcgagaa cttcaaacgc
      901 gacttcgtct atgtgggcga cgtggctgat gtgaatctgt ggttcctgga aaatggcgtt
      961 tccggcatct tcaatctcgg tactggtcgt gcggaatcct tccaggcggt agctgatgct
     1021 acgctggctt atcacaagaa aggccagatc gaatacattc cgttcccgga taaactgaaa
     1081 ggccgctatc aggcgttcac tcaggcagat ctgacgaatc tgcgcgcggc gggttacgac
     1141 aaaccgttca aaaccgttgc tgaaggtgta acggaataca tggcctggct gaatcgcgac
     1201 gcataagagc tctgcatgaa aatactggtg atcggcccgt cttgggttgg cgacatgatg
     1261 atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc cccaggcgat aatcgatgtg
     1321 atggcaccgg catggtgccg tccattatta tcgcggatgc cggaagttaa cgaagctatt
     1381 cctatgcctc tcggtcacgg agcgctggaa atcggcgaac gccgcaaact gggtcatagc
     1441 ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccca actccttcaa atctgcatta
     1501 gtgcctttct tcgcgggtat tcctcatcgc accggctggc gcggcgagat gcgctacggt
     1561 ttactcaacg atgtacgcgt gctcgataaa gaagcctggc cgctaatggt ggaacgctat
     1621 atagcgctgg cctatgacaa aggcattatg cgcacagcac aagatctgcc gcagccattg
     1681 ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat atacctgtaa tcaattttcg
     1741 ctttcatcag aacgtccgat gattggtttt tgcccgggtg cggagtttgg tccggcaaaa
     1801 cgctggccac actaccacta tgcggagctg gcaaagcagc tgattgatga aggttatcag
     1861 gtggttctgt ttggctcggc gaaagatcat gaagcgggca atgagattct tgccgctttg
     1921 aataccgagc agcaggcatg gtgtcggaac ctggcggggg aaacacagct tgatcaagcg
     1981 gttatcctga ttgcagcctg taaagccatt gtcactaacg attctggcct gatgcatgtt
     2041 gcggcggcgc tcaatcgtcc gctggttgcc ctgtatggtc cgagtagccc ggacttcaca
     2101 ccgccgctat cccataaagc gcgcgtgatc cgtttgatta ccggctatca caaagtgcgt
     2161 aaaggtgacg ctgcggaggg ttatcaccag agcttaatcg acattactcc ccagcgcgta
     2221 ctggaagaac tcaacgcgct attgttacaa gaggaagcct gacggatgcg ggttttgatc
     2281 gttaaaacat cgtcgatggg cgatgttctc catacgttgc ccgcactcac tgatgcccag
     2341 caggcaatcc cagggattaa gtttgactgg gtggtggaag aagggttcgc acagattcct
     2401 tcctggcacg ctgccgttga gcgagttatt cctgtggcaa tacgtcgctg gcgtaaagcc
     2461 tggttctcgg cccccataaa agcggaacgc aaagcgtttc gtgaagcgct acaagcagag
     2521 aactatgacg cagttatcga cgctcagggg ctggtaaaaa gcgcggcgct ggtgacgcgt
     2581 ctggcgcatg gcgtaaagca tggcatggac tggcaaaccg ctcgcgaacc tttagccagc
     2641 ctgttttaca atcgtaagca tcatattgca aaacagcagc acgccgtaga acgcacccgc
     2701 gaactgtttg ccaaaagttt gggctatagc aaaccgcaaa cccagggcga ttatgctatc
     2761 gcacagcatt ttctgacgaa cctgcctaca gatgctggcg aatatgccgt atttcttcat
     2821 gcgacgaccc gtgatgataa acactggccg gaagaacact ggcgagaatt gattggttta
     2881 ctggctgatt caagaatacg gattaaactt ccgtggggcg cgccgcatga ggaagaacgg
     2941 gcgaaacgac tggcggaagg atttgcttat gttgaagtat tgccgaagat gagtctggaa
     3001 ggcgttgccc gcgtgctggc cggggctaaa tttgtagtgt cggtggatac ggggttaagc
     3061 catttaacgg cggcactgga tagacccaat atcacggttt atggaccaac cgatccggga
     3121 ttaattggtg ggtatgggaa gaatcaagta gagtgtcgtt ctacaagtat gtctcttgca
     3181 gatttgccag ctcaaaccgt ttttcaaaat ttaaatcttg aaataataac caataagttg
     3241 acatcggaga taagatgacc tcaacattat ttttctctct cgagaaaaaa aactggatag
     3301 cgtactggaa cagagctctc gtattcttat tcattaccac ctattttttg ggtgggatta
     3361 caaggtataa acatcttatt gttattctta tgacaataac gacaatcgtc tatctctgca
     3421 aacggccaaa acactatctc tcactattta aaacatttct ttttggtagt gttgccatat
     3481 taactattgc tgcattgctg tcacttcttc aatcccctga tgcaggtgct agcatgaagg
     3541 aaattttcaa agctattatt gagaatactt tactatgcac aatagcaata ccgatcatat
     3601 tgagagacga gaaaagagaa gatgtcgaaa aaatcgtttt cttctcattt attagtgcgt
     3661 tgggcttacg ctgtttttct gaattgatta cctattataa ggactatcaa caagggataa
     3721 tgccattcgc agattataga caccgtagca tttctgactc gatggtcttt ttattccctg
     3781 cattgttaaa tctctggctt atcaaatcag caaaataccg catttctttt gtggttctaa
     3841 gcgttatttt tatttttctg atattaggaa ctttatccag aggggcctgg ctttccgtgt
     3901 tagtcattgg attaatatgg attctgatgt ttaaacaatg gaagttacta ttagtaggag
     3961 taatggttgc catcattgca ttgtcggtta ttttcacaca taaggagatg accgcaaagc
     4021 taacgtataa acttcaacaa actaatagtt cttatcgcta tgcaaatggt actcaaggca
     4081 gcgcactcga tctaatatta gaaaatcctg ttattggtta tggttacggt aacgttgcat
     4141 ataaagatgt ctataataaa cgtgtcattg attatccaga atggaccttt agacaatcaa
     4201 tagggccaca taattttgcg ctattcatct ggtttggcac tggtttatta gggctggtaa
     4261 gtcttatgat gctatactgt gcaatattga aagagtgtat aaaaaatggc gtcaagaata
     4321 aatatcgctc accatataat gcatattata taatcttact atcttttata ggttattttg
     4381 ttatccgtgg aaacgtagaa caaattgaac caaatttatt aggcgtttac gccggcttat
     4441 tattagcgat gaaaaacaag taaaaaataa aaaaggctgc ataatgcggc ctttttattt
     4501 attcaaacca attatgaata acctcttcga atctttgagt tacgccatcc caactgtagt
     4561 gatcaaacac aaagtcctga ccttgctttg caactgctgt taattcagga ttcgctaacg
     4621 tttttaaaat atcgctgctt atggagtctg cagtcatagg ttcttttaaa tggaatcccg
     4681 tagtattttc tttgacaaat tccgtcatcc ccccacgcgt actcactaaa acaggcttac
     4741 cagcacccat agcttcaatt gccaccatgc aaaatggttc ctggaactgg gaaggaatga
     4801 cgactaaatc tgccagtggg taataacaat gcatcttttc aggcggtatg cttcctaaca
     4861 taatgcaacg atccttcaaa cgttttgcaa tttccctgac attgcgttga tatgcacctt
     4921 tgtcactttt actcatttct gtataatcac caatcacaac taattttaaa tttttgtgag
     4981 cagcggccaa tttttcgaat gattgcatca acagcaatat gcctttgtca gggacgatac
     5041 gcccagcata aaaaataatt ttctcttcag gggagatatt aagttcgctt ctagtgatcg
     5101 gctgaaaatt agattgatag gtttctaaat caattccatt tggtacaatc tcaatatctg
     5161 catttgctaa ataagattgg taatactttt tcaagtataa actaggcact atcattttaa
     5221 cattttgctc aagcaatttt ggttcaaatg cattatgcat atgtataacc actcttgcct
     5281 gaggagctcg cttacgaatt tgagtgtata gcttcatgct attatgaact acaataacac
     5341 tttctttcgt tataggaaaa tcatgtgcaa tattcaatat tctctgtgaa tatggaagag
     5401 gatcaagtct tgtccatttt tgaaataacc gcttatatat gcgactgaat ccaaccctat
     5461 gaatactaca attatcatta acaaaggtgt aatttgagta tcctggattt ttgatacaaa
     5521 caatacgatt tggaaaattg gtacgctgag caacctgata catccacgtt tcaacagcag
     5581 ccgctccgcg aggaggaatt gaaaaaatag gagtaaccgt aaatattatt ttatcaacca
     5641 tctcgtatat taacctttca taacattata tttaattgct gtaaaaacac catcgagaaa
     5701 ttttttctgg taaagcaagt gtttatattt ttctttgtac tcatgttttt taacagctt
//
LOCUS       DBBGLQ010000191         5725 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.191, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000191 DBBGLQ010000000
VERSION     DBBGLQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.191"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(103..1122)
                     /locus_tag="ACIYSB_005101"
     CDS             complement(103..1122)
                     /locus_tag="ACIYSB_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGC5431948.1"
                     /translation="MAITRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLR
                     EKIEAAVNELGYMPNLTASALASASSHTIAMVVPNLAEAGCTEMFAGLQHVLQPAGYQ
                     IILAESRHRLEQEEKLLETLLASNIAAAILLSVEHNSTVRHLLKNASIPVIEIGAIRT
                     DPIDINIGIDNVAAMFELTDMLVQRGYQNIGLLCANQEQWIFQQHLQGWYKAMLRHHM
                     SPNRVINAALPPVFSTGASQLPEFLLAWPELDALVCVSDELACGVLYECQRRRIKVPD
                     ELAVVGFGDSDVSKVCQPSLTTMAVPHRKIGLEAGNVLLERIKEGNWSNQIFIEPCLC
                     LRESC"
     gene            1380..1823
                     /locus_tag="ACIYSB_005102"
     CDS             1380..1823
                     /locus_tag="ACIYSB_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGC5431949.1"
                     /translation="MLNIWLSDSTIALRKNAATWQQALDICARPLLDAEIITTDYLSA
                     IKTQHEKLGPYYVLAPGLAMPHARPEEGAKGLGLSLLKLEEGVSFHTEEFDPVDVIIL
                     LAAPDKHSHIEMISALAELFSSEKDLHELHQARNVEEIKKIISRF"
     gene            1902..2174
                     /locus_tag="ACIYSB_005103"
     CDS             1902..2174
                     /locus_tag="ACIYSB_005103"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGC5431950.1"
                     /translation="MKIMAICGSGLGSSFMVEMNIKKVLKKMDIEAEVEHSDLSSATP
                     GAADLFVMAKDIAASASVPESQLVVINNIIDINELETQLRNWLAKQ"
     gene            2197..3585
                     /locus_tag="ACIYSB_005104"
     CDS             2197..3585
                     /locus_tag="ACIYSB_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGC5431951.1"
                     /translation="MFILETLNFVVDILKVPAILVGLIALIGLVAQKKSFSDVVKGTI
                     KTILGFIVLGGGATVLVGSLNPLGSMFEHAFNIQGIIPNNEAIVSIALEKYGASTALI
                     MAFGMVANIIVARFTRLKYIFLTGHHTFYMACMIGVILTVAGFEGISLVFTGSLILGL
                     VMAFFPALAQRYMKRITGNDDIAFGHFGTLGYVLSGWIGSVCGKGSRSTEEMNLPKNL
                     SFLRDSSISISLTMMVIYLIMAISAGREFVESQFSGGQNYLVYAIIMAITFAAGVFII
                     LQGVRLILAEIVPAFTGFSEKLVPNARPALDCPVVYPYAPNAVLVGFLFSFFGGLVGL
                     FLLGQMKLVLILPGVVPHFFTGAAAGVFGNATGGRRGAMIGAFANGLLITFLPVLLLP
                     VLGAIGFANTTFSDADFGAVGIVLGNLARYLSPFAITGLVVALFVLLVAWNIFASNKI
                     AAKAQKKSGAKS"
     gene            3582..4412
                     /locus_tag="ACIYSB_005105"
     CDS             3582..4412
                     /locus_tag="ACIYSB_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGC5431952.1"
                     /translation="MNVKELTQFARDIRIQTLKSLTHLGFGHYGGSMSVVETLAVLYG
                     AVMKIDPAAPDWPERDYFILSKGHAGPALYSTLALKGYFPLEELSTLNQNGTRLPSHP
                     DRLKTCGVDSTTGSLGQGISIAGGMALSHKLAGRKNRVFCIVGDGELNEGQCWEAFQF
                     IAHHRLNNLTVFVDWNKQQLDGQLDEIINPFDLEGKFRAFGFDVVTVKGDDIEGLLNV
                     VKPVLPAEARPRVVILDSIKGQGVPCLEQLSNSHHLRLTEEMKGTLNETIRQLEAMHD
                     "
     gene            4405..5358
                     /locus_tag="ACIYSB_005106"
     CDS             4405..5358
                     /locus_tag="ACIYSB_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGC5431953.1"
                     /translation="MINLAPAGQKDSIEMRKVYADFIAQQSKNVSSVIALEADLMSSM
                     AMDSVAQDYPDHVINCGIMEANVIGTAAGLALTGRKPFVHTFTAFASRRCFDQLFMSL
                     DYQGANVKVIASDAGVAACHNGGTHMSFEDMGIVRGLAHSVVMEMTDAVMFSDILRQL
                     VALEGFYWIRTIRKQAASIYAPGTTFTIGKGQVLREGTDVTLIANGIMVVEALEAARQ
                     LELAGVSVAVIDMFTLKPVDRMLIKNYAEKTGRIVTCENHSIHNGLGSAVAEVLVETC
                     PVPMRRVGVKERYGQVGTQDFLQREYGLTAQDIVAAARELL"
     gene            5547..5725
                     /locus_tag="ACIYSB_005107"
                     /pseudo
     CDS             5547..5725
                     /locus_tag="ACIYSB_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1464 a         1217 c         1401 g         1643 t
ORIGIN      
        1 tcacgcctta tgatgacacc cttcccctgg ttgtggaaaa ccgaaggata tatcaggttc
       61 agcaacaaaa tcagtgagct gaaagatacc agtcccccag cttcagcagc tttcacgtaa
      121 acataaacag ggctcaatga atatctgatt gctccagtta ccttctttaa ttcgttccag
      181 caaaacattt cctgcttcca gaccaatttt cctgtgagga acggccattg ttgttaatga
      241 tggttggcaa actttgctga cgtcactgtc accaaatcct actacggcga gctcgtctgg
      301 aactttgatc cgtcttcgtt gacattcata gagaacaccg caggctaact catctgacac
      361 acataccagg gcatccagtt ccggccatgc gagcaaaaat tccggtagtt gtgatgctcc
      421 ggtggaaaaa acaggtggca gagcagcgtt aataacacgg ttgggagaca tatgatggcg
      481 taacatagct ttataccatc cctgtaaatg ctgctgaaag atccactgtt cctggttagc
      541 gcatagcaaa ccgatattct gataaccacg ttgtaccagc atatccgtca gctcgaacat
      601 tgctgcaaca ttatcaatac caatgttgat atctatcgga tcagtacgaa tggcaccgat
      661 ttctataaca ggaatcgagg catttttgag caagtgacgc acggtactat tatgctcaac
      721 actaagcaaa attgctgcag cgatattaga agccagcagg gtttccagca gtttttcttc
      781 ttgttccaga cgatggcgtg actcagccag gattatttga taccccgctg gttgtagcac
      841 atgttgcagc ccggcaaaca tttccgtgca tcccgcttca gccaggtttg gcacgaccat
      901 tgcgatcgtg tgtgaagatg ccgatgccag tgcgcttgca gtgaggttgg gcatataccc
      961 taattcatta actgcggctt caatcttctc cctcaattta tctgaaactt gttcgggagt
     1021 gcgtaacgct ctggatacag tcattgtgcc aactcccgcg agttgcgcga catcagcaat
     1081 agtgacttta cctgtactac gacgttttcg ggttattgcc atacaaaatc ctgctcagcg
     1141 actacatatg gagatctttt attacttatc atcaacggct tcctgcactg ccagtagcag
     1201 agtatacctc ttaatatctg ccataagtct ggatttaatc agtgaaatct attttgatag
     1261 cgctatcaca aattcgaaca atacctcctg atagcgctat ctttgtgata ttgatcgcag
     1321 atataccgtc gttacttgaa tacattctgg tcatataaat aagaaattag ggggtgggag
     1381 tgcttaatat ctggctttct gattcaacaa tcgcattacg aaagaatgcc gcaacctggc
     1441 agcaggcatt agatatctgt gccagaccgt tactggatgc agagataatt acgacggatt
     1501 atctcagtgc gataaagaca caacatgaaa aactggggcc ttattatgtc ctggcacccg
     1561 ggctggccat gccgcatgcc cgaccagaag aaggggcaaa aggattaggt ttatcattat
     1621 taaaacttga agaaggtgtc agttttcata cagaggaatt tgatcctgta gatgttatca
     1681 ttttacttgc cgcaccagac aaacacagtc atatcgaaat gatatctgca ctggctgagt
     1741 tattctccag cgaaaaagat ttacatgaat tgcatcaggc tcgcaatgtg gaggaaataa
     1801 aaaagataat ttcacggttc tgattacatt ataaattcag tcttttcaca acattgcgcc
     1861 gttgcgtgat gggacttctt caacttaaaa tggtgaatat aatgaaaatt atggctattt
     1921 gtggctcagg gcttggtagt agttttatgg tcgaaatgaa tattaaaaaa gttctgaaaa
     1981 aaatggatat agaggcggaa gtggaacatt ccgatctctc atcagcaacg ccaggggctg
     2041 ccgatttatt tgtgatggcc aaagatattg ctgccagtgc cagcgtaccg gaaagtcagt
     2101 tggtggttat caacaacatc attgatatta atgaactcga aacgcaactg cgtaactggt
     2161 tagcaaaaca ataaccttaa ttgaagcgag gtggatatgt ttatccttga aacgctgaat
     2221 tttgttgttg atatacttaa agtgcccgcg atactggtcg gcttgattgc cttaattggt
     2281 ctggttgccc agaaaaaatc tttttcagat gttgttaaag gtacaattaa aactattctc
     2341 ggctttattg tactgggcgg tggtgccact gttttggttg gttcgttaaa tcctcttggg
     2401 agtatgtttg aacatgcctt taatattcag ggcatcattc cgaataatga ggctattgtt
     2461 tctattgcgc tggaaaaata tggcgcatcg acggccctta ttatggcatt tggtatggtg
     2521 gcgaatatta ttgttgcccg ctttacccgc cttaaatata tcttcctgac cggacaccac
     2581 acattttaca tggcctgcat gattggtgtg attctgacag tggctggttt tgaaggtata
     2641 tcactggtct ttaccggttc tcttattctg ggacttgtga tggcattttt cccggcatta
     2701 gcacaacgct atatgaagcg tattacgggg aatgatgaca ttgcttttgg tcactttggc
     2761 actctgggat atgtactttc tggctggatt ggcagcgtgt gcggtaaagg ttcccgctcc
     2821 accgaagaga tgaatttgcc caaaaatctg agcttcctgc gcgacagttc catctctatt
     2881 tctcttacga tgatggtgat ttatctgatc atggcgatca gtgctggtcg tgagttcgtt
     2941 gaaagccagt tcagtggtgg tcaaaactac cttgtctacg ccatcataat ggccatcacc
     3001 ttcgctgctg gtgtatttat tattctgcaa ggcgtgcgtt taatccttgc tgaaatagtt
     3061 ccggcattta ccgggttttc agaaaagctg gtgccgaatg cccgtccggc cctcgattgc
     3121 ccggttgtgt atccgtatgc gccaaacgca gtgttagttg gtttcttgtt tagctttttt
     3181 ggcggtctgg tagggctatt tttgttggga cagatgaaac tggtgttgat tcttccgggt
     3241 gtcgttcctc acttttttac cggggcggct gctggcgtat ttggtaacgc tacgggggga
     3301 cgtcgtggcg caatgattgg tgcttttgcg aatggcttat tgattacctt cctgccagta
     3361 ctgttattgc cggttcttgg ggcgataggc tttgctaata cgaccttttc cgatgcagat
     3421 tttggtgctg tcggcatcgt cctcggaaac ctggcacgtt atctctcacc ttttgccatc
     3481 accggacttg tagtagcgct atttgtgctg ctggttgcct ggaacatttt cgccagtaac
     3541 aaaatcgctg ccaaagcgca gaagaagagt ggagccaaat catgaatgtt aaagaattga
     3601 ctcaatttgc acgggatata cgtatccaga cgcttaaatc attaactcat ctcggcttcg
     3661 gtcattatgg tggcagcatg tcggttgtgg aaacgctggc agtgctttat ggcgctgtaa
     3721 tgaaaattga tcctgctgct cccgactggc ctgagcgtga ctattttatc ctgtccaaag
     3781 gacatgccgg acctgctctc tacagtactc tggcgctgaa aggctacttt ccactggaag
     3841 aattaagcac cttaaatcag aacggaaccc gcttaccaag tcatccggat cggctaaaaa
     3901 cgtgtggtgt tgatagcacc acaggatcgc tggggcaggg gatatccatt gccggaggga
     3961 tggcgctgtc gcataaactg gcggggcgca agaatcgggt tttttgtatt gtcggcgatg
     4021 gcgaacttaa tgaaggccaa tgttgggaag ctttccagtt tatcgcgcat catcgtttga
     4081 ataacctcac tgtgtttgtg gactggaaca aacagcagct tgatggtcaa ctggatgaaa
     4141 ttattaatcc atttgatttg gaaggaaaat tccgggcttt cggttttgat gtcgtgacag
     4201 tgaagggcga tgatattgag ggcttgttga atgtagttaa acctgtcctg ccagctgagg
     4261 ccagaccgag agtggtcatt ctcgatagca ttaaagggca gggggtgccg tgtctcgagc
     4321 aactcagtaa ttctcatcac ttacgcctca cagaagagat gaaggggacg ctaaacgaaa
     4381 caattcgcca actggaggct atgcatgatt aatctcgcac cggcaggaca aaaagacagc
     4441 attgaaatgc gcaaagttta tgcagatttt atcgcgcaac agagtaaaaa cgtcagttct
     4501 gtcattgctc ttgaggctga tttaatgagt tccatggcaa tggacagcgt ggcgcaggat
     4561 tatccagatc acgtcattaa ttgcggcatt atggaagcca atgttatcgg cacagctgca
     4621 gggctggcct taaccgggcg caagccattc gtgcatacct ttaccgcatt tgccagccgt
     4681 cgctgttttg atcaactctt tatgtcgctt gattaccagg gcgctaacgt caaagttata
     4741 gcttctgatg cgggtgttgc tgcttgccac aatggcggaa cacatatgtc atttgaagat
     4801 atgggaattg tgcgcgggct ggcgcattcg gtggtgatgg agatgacaga tgccgtaatg
     4861 ttcagcgata tcttgcgtca actcgttgca ctggaaggat tttattggat tcgtaccatt
     4921 cggaagcaag cagcgagtat ttacgcacca ggtacaacct ttacaattgg caaagggcag
     4981 gtattacgtg aaggtacgga tgtcacctta attgctaatg gcattatggt ggtggaagcc
     5041 ctggaggccg cacgtcaact tgagctggct ggggtaagtg tagcggtgat tgatatgttt
     5101 acgctcaaac ccgttgatcg gatgttaatt aaaaattatg ccgaaaaaac aggacggatc
     5161 gtaacatgtg aaaaccatag tattcataat ggattaggtt cagcggttgc tgaagtgctt
     5221 gtagaaactt gtcctgtgcc catgcgtcgg gtgggggtta aagagcggta tggccaggtt
     5281 ggaactcagg actttttaca gagagagtac gggttaaccg cgcaagatat tgtggcggca
     5341 gccagagagc tattgtaatt catggagttt gtttaccatt ctgtttttct taaaagtgag
     5401 tgtctgtgcg gtcactaaac tcggtaataa agcagttcat tgcaggataa gaagcgtttg
     5461 gttgtgtcgt tttaccgtaa gaaccgaccg ccgtatgtag ccattaagcc agtattggta
     5521 acgtaggtgc ccaccttttc aaactggtgg acgcctgtta tggagcaaaa acattatccg
     5581 cagaaccccg cagatcattt tcaaatgagt taaacttcaa atggttaaac tggttttgca
     5641 accaggagcc tctgttgccc gtattgcccg ggaacacgat atcaatgata acctcctgtt
     5701 caaatggctc agactctggc agaac
//
LOCUS       DBBGLQ010000192         5700 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.192, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000192 DBBGLQ010000000
VERSION     DBBGLQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.192"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            229..435
                     /locus_tag="ACIYSB_005108"
     CDS             229..435
                     /locus_tag="ACIYSB_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5431954.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            529..1131
                     /locus_tag="ACIYSB_005109"
     CDS             529..1131
                     /locus_tag="ACIYSB_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431955.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(2090..2389)
                     /locus_tag="ACIYSB_005110"
                     /pseudo
     CDS             complement(2090..2389)
                     /locus_tag="ACIYSB_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2440..2595
                     /locus_tag="ACIYSB_005111"
                     /pseudo
     CDS             2440..2595
                     /locus_tag="ACIYSB_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3726..4877
                     /locus_tag="ACIYSB_005112"
     CDS             3726..4877
                     /locus_tag="ACIYSB_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431956.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            4961..>5700
                     /locus_tag="ACIYSB_005113"
     CDS             4961..>5700
                     /locus_tag="ACIYSB_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGC5431957.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGPREVQRFRLNGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILLELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVMQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHRVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFT"
BASE COUNT         1521 a         1484 c         1422 g         1273 t
ORIGIN      
        1 agccagtaat gggttaagtg ataacaggtg tctggaaata taggggcaaa tccaaccccc
       61 ctccgggatt ccgctacggc tctgccatcg caatcccccc gtttatctcc ggacagccac
      121 attcccgatt attttttacg tttctccccg gttgttatgc cggtgaaggt ggcgcgctgt
      181 tttcatgacc acaccggctg gtttaacaac atccggagga acattctcat ggctacccca
      241 gtttcgctga tggatgacca gatggtcgac atggcgttta tcactcaact gaccggccta
      301 accgataagt ggtttgacaa actcatcaaa gatgggggct ttcctgcgcc catcaaaatg
      361 gggcgcagct cccgctggct gaaaagtgaa gtggaagcct ggctgcaggc gcgtattgca
      421 cagtcccgtc cgtaattttt aaccactaac ccttcgccca cagcagacgc aatctccggc
      481 ctgccggcgg cgctctgctg cctgttatcc cccgtgagga atatgaaaat gaaacaacag
      541 tatcagaccc gttacgagct gctccatgaa aactaccaga aatggctgac cggcttcacc
      601 cggcacgccg tatcatgggg cgtgtgtcat ccgaatatct actatttcca taatctgacg
      661 cccgggtggg tgtcattcaa tggcgaaaaa ccggagattg ccatcgttcc ccagagtctg
      721 caccggctga tttatggtcc ggacaaacgg agctccccgt ccctggatga tgatctggtg
      781 gtgaatttat gcaccagcga acacctgctg gttcatcatc cgatgctgga agggatcctg
      841 ctgtctgaat gtgagcgcct gaagcaacat tctctggcaa acaaactgat cagcctcttc
      901 cgtcagtttg gcggcacgga gttacgtctc aaactggtct ggctctgctg gcttgactta
      961 atgaccggaa acagccttga cgactggacg aaaaacctga aacacaaatc agaaaaagat
     1021 ctggagcaat ggatcattgc acgtcagggt cagagtgaac cactgacaaa tctgatggat
     1081 cagtacgtgc ttatggcata ccgtacatcg gttgatgccg ctcacagttg atgtctgaga
     1141 accacagggc tggcagcaga aaaccagccc tgtggtaaaa tacagtaata gcggcgtaca
     1201 tcaggcacgc cgctcactcg tccacagatc atatacgtca ttatactgcg cgataacgga
     1261 gggcttcagc acccggtcat ttctgagcgg gtatgaatac cattgatgat gcgaagcagc
     1321 cacgggcaga aacgttcttc acaaccaggt gcctccatct tcactgcagt ctgaatgtgc
     1381 cggcttgtta tccaccagaa atggtatgct ccggtacatg cataccccgg agactataaa
     1441 cgcactgaat catagcctcc cacctcagca ggaggcagtc agctttcagg cggaaaaacg
     1501 ttgcagttaa agatcgtgag aatcgctgtt tcgttgcata gcaacccgaa cagttgtagt
     1561 gaaacatttt acctgcatca gcacatggtc aggcgccgtc tgtatggcgt ggaatactga
     1621 tgtttcggct gatggcaagc ctcattctct gtggaaacag gggcgcgcat tagcttctca
     1681 accagagagt gtcacaaaga caaatattgc tgaaaatcgc attcatcgtt caggcgtgaa
     1741 tgcccggaga caaaagccgc tgtccacaca gtccccctct gacctgaaca aaaagcatac
     1801 acacaacagg ctcacctgac cccgaaaaac cataacttca gcaataccag tgacaccagc
     1861 acgttacctc agtgccacat cttcagaccg cgatcaatct gaaatgtgac tatcggggca
     1921 atatccctgc tttgtggtac acgataacca gaaaacatcc cctgaaccgc cagccagaaa
     1981 gactggccac aaaaggggta agctgaaatc gtttatggat tacatcgaca tatgcctggc
     2041 agagaatggt aagcgtgcag tgagaaccgt attgacgaag tgaagctggt caggcaggca
     2101 gagtaacccg ccacgggagc agagcaccca cccggttcac aggccagtcg gcgatgacat
     2161 caagtacatg atgcagatag cgttccggat ccacgccgtt taatttgcac gtcccgataa
     2221 gactgtacag tgacgcaccg cgctcaccac catgatccga gccgaagaac agccagtttt
     2281 ttctccccag actgaccagt cgtagggcgt tttcggcaat gttattatcc acctccaccc
     2341 agccatcttc tgcatagcgg gtcagcgccg gccactggtt cagcgcatac cacctctccg
     2401 ccacacccgg gaagaagttg atatggtggt tgccggacaa cagcgctcct ggattgactg
     2461 ctgcatcact caaccacgct gaatatcaaa ggagagcgtt accggttaaa agagaaacgt
     2521 aaagctgtgg tgatgaccca aaacgcaacg cctgtcagtg aggatgaaat ggtgaaaagc
     2581 ggacagcatc agtaacggaa gtatcttagc gggtacgaaa atggcaggat cgtgacattg
     2641 acagaatatc tcctgatcat cctgaatgac tgcagttgct gtagaaaagt aagctgtctc
     2701 cacccacggc accacttaac aggcattccg taacttcccc cagacttact atgacgcatt
     2761 atcggtgcac tgaaaacggg agagtgcgct taaaaccgca gaccgcatgc gggtgttact
     2821 gcatgacagt ctggccgtat tgtgagatgt cggcaggaac cgatgtccgc gcagggggga
     2881 acttcctgca gtacctgacg cttcaaatgg tattaaccgt agggtgttgt tggttccgga
     2941 tcgcggaaat gggagcacag gtctgctacg gggtgtacct ctgacagtat atccggctcg
     3001 ctcatctgac agcctctctc ccggaaaaga ccaaaaatga gatcgtctcc tcaacagcag
     3061 agaagaggtc ttgacagaca ggctatattg catacgattt atgaagaaaa agacagcaaa
     3121 tatggaggtt atagcggaga ataccctacg aaattcctat gaaaaacgat tgagaaaaat
     3181 atcaaattcg attcgttttt atatgctttt tgggagcgtg gcgatgacga actgttgcat
     3241 gtgcagagga gaatgcaccc ggaagcacca gaatcatcca gaaaggcaga acgattacgc
     3301 atcaaaccag gagacagcaa tatcaaggta atatactgat ttaaaatgaa tttacagcaa
     3361 tacgtgaaaa cattttttac ttaaaggaga cagatgcgct gaagagagtg caggtatttt
     3421 tatataaaaa tgagaagttt acgggatgta tatccggcac actatgaccg gagaatatca
     3481 gtcaagctta aaaagacaat aaatacagtg agttaaatac acagagaaaa cgtagtatca
     3541 gagggtacat cttacaacag agactgaatt tccggttttt cataaccggc gcattccgga
     3601 aagtgcaggc ggcccggagg ttttctgacg ccactacctg gtagattgtt ctgtacatca
     3661 tgtacggagg tttttgtttg cttttttaaa atctgcaaat gtgagaaaag caggtgaggt
     3721 gtattgtggg aaaggaactg gatgatatct gcaccagctg tccgtatatc gatgctgtaa
     3781 aacggcacaa acaacagctc ggtgccatag aggaatatac acagtggtta aaaaaagaac
     3841 cacgggcatc ttattttttt ctgttccagc tgtatacccg cattcacaat acatttttct
     3901 cccaaaagca acaactcccg ttcactcccg gtggcactca ctgcccggaa cctgatgtca
     3961 cccttcggga cttaacgctt tcccctggct accacagtga ttatgcccct caacccattc
     4021 cggagatgga cagttctgct gttgttcccc caactaatga aaacacctcg cctccggaag
     4081 atactcccga caacactccg gccggaggca ataccggaca agcggaaaag accagaaacg
     4141 caggactcac acccattcct gaaaaacgtt caggaatgcc cccggaacat ctgcggtttg
     4201 ccacggggtt tccatcgcaa ccgaagattg ccggtcccag agaaaagccg atgcgcaccg
     4261 tgcatccgga taaaatctac cgtgaaatta tatggttctg ctccagttac ctgctgaaat
     4321 ccggaccaga ggctacgagg acgattatca actcagtatt ctctgaatgg gccagcataa
     4381 acaatgatta cccctccccc ttttcgtggg tggacagcag ggacagtgaa cagtgtgact
     4441 ggttatggaa cgccatgcag gtccggtgtg tgggaacccc gctgaatccc cttaccccgg
     4501 agcagaaata ctggtttgcc tgcgccacgt ttgataactg ggagggctgg aatgagcaac
     4561 aggtacagtt tttactggaa agtaatccca gacggaacag agcgaagttt acgcaggtct
     4621 ccttccaggc ccccaggatt cagcataaag cgattcttct tgatgagctg aaaagtgccc
     4681 gggagcaaca aaaaaggcgt gatgaacgtg ctgatggttc cgtccctctg aaactgtccg
     4741 gaaaaatcca caaacagctt gaaagtattg cccgaagtcg tggtgtcctc ccaaaaaaac
     4801 tgctgaatga aatgattgag caggcgtacc atgatttggt ggcaaccagg cagaacagcc
     4861 aaatcgattc cagataattt cagacagata ttatttccgt gaatcccccg ccacctttcc
     4921 ggtgcgcggg gttttgtctt ttttcaccgg gaatacatgt atgaatccgt ctgatgccat
     4981 tgaggcaatt gaaaaaccgc tctcctccct gccttactca ctttcccgtc acatcctgga
     5041 acatctgcgc aaactcacca gtcacgaacc cgtgcctggc attatgggta aaagcggggc
     5101 cggtaaatcc tcactctgta atgcgctgtt tcagggggag gtcaccccgg tcagtgatgt
     5161 tcacgccggc ccccgggaag tgcagcgctt ccgtctgaat ggccatggtc acagcatggt
     5221 tatcactgac ctgcccgggg tgggcgagag ccgggacagg gatgcagagt atgaagccct
     5281 gtaccgtgac attctgctcg aactggacct ggtactgtgg ctgattaaag ccgatgaccg
     5341 tgccctgtct gtggatgagt atttctggcg acacatcctg caccggggac atcagcgggt
     5401 actgtttgtg gtgatgcagg ccgataaaac ggagccctgc catgaatggg atatggccgg
     5461 tattcagcct tctcccgcac aggcacagaa cattcgcgaa aaaacggagg cggtattccg
     5521 tctgttccgg cccgtacatc gggttgttgc cgtatcggcc cgcaccggct gggaactgga
     5581 tacgctggtc agtgcgctca tgacagcact tcccgaccat gccgccagtc ccctgatgac
     5641 ccgcctgcag gacgagctgc gcacggagtc tgtccggtct caggcccgtg aacagtttac
//
LOCUS       DBBGLQ010000193         5597 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.193, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000193 DBBGLQ010000000
VERSION     DBBGLQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.193"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            10..513
                     /locus_tag="ACIYSB_005114"
     CDS             10..513
                     /locus_tag="ACIYSB_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5431958.1"
                     /translation="MLASTVSFGVNAANQGQGVINFKGTVIDAPCGIAQESADQTINF
                     GQISKAHLNADGISVKKDLDIKLVNCDATEIGKLTNGVKVSFTGTTINGQNQELGTTG
                     DTGTAIVVSAANGSLVSFDGTAGSATKVKEGDNTLRYSTWVKKATGGTLKEGDFTAVA
                     NFNLAYE"
     gene            600..1187
                     /locus_tag="ACIYSB_005115"
     CDS             600..1187
                     /locus_tag="ACIYSB_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5431959.1"
                     /translation="MFWRMIISVPFFWCAMAQALPSGSFPPPGMTLPEYWGEEHVWWD
                     GRASFKGQVIAPACTLAMEDAWQEIDMGTTPLRDLQNSPAGPEKKFRLRLRNCELAGA
                     GKQVYTATRVRVTFDGIPGETPDKFSLTGQAEGINLQIMDNYGYPARAGKSMPPLILR
                     GNEDGLDYSLRIVRNGYPLKAGDYYAALRFKLDYE"
     gene            1308..3818
                     /locus_tag="ACIYSB_005116"
     CDS             1308..3818
                     /locus_tag="ACIYSB_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGC5431960.1"
                     /translation="MKKNKSTFTINFITYSLMLSLAGVPVYAVDFNTDVLDAADRQNI
                     DFSRFSRAGYIMPGQYQMEIRVNDQDISPSAFQIAFLEPPFSDSDNEKPLPEPCLTPE
                     IVSRMGLTEASQEKVTYWNNGQCADFRQLSGVEIRPNPAEGMLYINMPQAWLEYSDAS
                     WLPPSRWDNGIPGLLFDYNINGTVNKPHKGKQSQSLSYNGTAGANFGAWRLRADYQGN
                     LNHTTGSGQGTDSQFTWSRFYMYRAIPRWRANLTLGENYINSEIFSSWRYTGASLESD
                     DRMLPPKLRGYAPQVSGIADTNARVVISQQGRILYDSTVPAGPFTIQDLDSSVRGRLD
                     VEVIEQDGRKKTFQVDTAYVPYLTRPGQVRYRLVSGRSRNYEHTMEGPVFAAGEASWG
                     ISNKWSLYGGGIVAGDYNALAVGLGRDLSEFGTVSADVTQSVARIPGEETKQGKSWRL
                     SYSKRFDDVNTDITFAGYRFSERNYMTMDQYLNARYRNDFTGREKELYTVTLNKNFED
                     WKASVNLQYSHQTYWDRRTSDYYTLSVNRYFDAFSFKNIALGLSASRSKYQNRDNDSA
                     FVRLSVPWGTGTASYSGSMSNDRYTNTVGYSDTLNNGLSSYSLNAGVNSGGGQPSQRQ
                     MSAYYNHNGSLTNLSASFSAVENGYSSFGMSASGGATVTMKGAALHAGGMNGGTRLLV
                     DTDGVGGVPVDGGRVYTNRWGIGVVTDVSSYYRNTTSVDLNRLPEDMEATRSVVESVL
                     TEGAIGYREFEVLKGSRLFAVLRLADNSHPPFGASVTNAKGRELGMVADSGLAWLSGV
                     NPGETLNVGWDGRTQCVVDIPAHPDPAQQLLLPCRQVK"
     gene            3887..4618
                     /locus_tag="ACIYSB_005117"
     CDS             3887..4618
                     /locus_tag="ACIYSB_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGC5431961.1"
                     /translation="MQKMKPALKKTLMAVACLSAVPAAQAAISLDRTRAIFNGDEKSM
                     TLNIANDNKQLPYLAQAWVENEKKEKITTGPIIATPPVQRLEPGSKSMVRLTSTPDIS
                     RLPQDRESLFYFNLREIPPKSDKANVLQIALQTKIKLFYRPESIKAKPNAVWQDQLVL
                     NKTGGGYQIDNPTPYYVTVIGIGGSEKQAREGEFDAVMLAPKSTQMVKSGTYSTPYLS
                     YINDYGGRPVLQFSCAGSRCTAVKK"
     gene            4655..5170
                     /locus_tag="ACIYSB_005118"
     CDS             4655..5170
                     /locus_tag="ACIYSB_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus-assembly fibrillin subunit"
                     /protein_id="HGC5431962.1"
                     /translation="MNRATIGFYLAVVLGSCSMNGVSQADELLTWDDFFVADESRHQW
                     VNEHNGRTGALNVKGALVSSPCQLDTNEVQISERKKRRDIQKVKINMSGCGYGNVPES
                     FSEWNRKLYVNASIIMSDLLRRACYLPSKRKFLSDGENQLIFYLECGTHIRSPEKVGR
                     EENALIRMSYD"
     gene            5211..>5597
                     /locus_tag="ACIYSB_005119"
     CDS             5211..>5597
                     /locus_tag="ACIYSB_005119"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5431963.1"
                     /translation="MLRKFIFLFVLFTFVNSVRAVDVPVRIYGTIIIPPCEINSGEPV
                     NVDFGNVQEEKINSRTYDKKIIVPVRCPYHQGDVSLTITAASIIENADVVATDIEGLG
                     ILLYEEGNNKPLSLNNAATISTGLRGK"
BASE COUNT         1481 a         1222 c         1544 g         1350 t
ORIGIN      
        1 attgcaacca tgcttgcttc tactgtttca tttggcgtta atgctgctaa tcagggacag
       61 ggcgttatta acttcaaagg tacagtaatt gatgctcctt gtggtattgc acaagaatca
      121 gcagatcaga caattaattt cggccagatt tctaaagcac atttgaatgc agacggaatt
      181 tccgtaaaaa aagatctgga tatcaagtta gttaactgcg atgccacaga aatcggaaaa
      241 ctgaccaatg gtgtgaaagt ttcttttact ggtacaacaa tcaatggaca gaatcaggag
      301 ttaggtacta ctggtgatac agggactgca atagttgtct cagctgcgaa tggcagcctg
      361 gtttcatttg atggtacagc aggttccgcg acaaaagtta aagaaggtga taataccctg
      421 cgctacagta cttgggttaa aaaagctact ggtggcaccc tgaaagaagg cgattttacc
      481 gctgttgcta acttcaatct ggcctacgag taatatgttg ggcaattctg tttgagatat
      541 ctgatatgtg cggggaatat gtccccgtac aattgccagt gagaagtaag gaataccgca
      601 tgttctggcg catgatcatt tcggtaccgt ttttctggtg tgcgatggct caggccctgc
      661 catcaggttc ttttccacca ccggggatga ctttgcccga gtactgggga gaagaacacg
      721 tctggtggga tgggcgggcc agctttaaag ggcaggttat cgctcctgcc tgtacgctgg
      781 ctatggagga tgcctggcag gaaattgata tgggaaccac gccactcagg gacttacaga
      841 acagccctgc cggtcccgag aaaaaattcc ggctacggct acgcaactgt gagcttgcag
      901 gtgcaggaaa gcaggtctat acggcaaccc gggtgcgtgt gacttttgat ggcattcccg
      961 gagaaacacc ggacaaattc tcgctgacag gtcaggctga aggtataaat ctgcagatta
     1021 tggataatta cggatatccg gcgagagcag gaaaaagcat gccaccttta attctcaggg
     1081 ggaatgaaga cgggcttgac tattctcttc gcattgtcag aaatggttat ccattaaagg
     1141 ctggcgatta ctacgctgca ttgcgtttta aactggacta cgaataaata caaccggagt
     1201 agccgggctc actgctatca gcgacagtgt taataataca ttcctgaatg ttgaagagag
     1261 ttagttgcgc ccctcaaagt gtttttgtat taaatcggct gcatataatg aaaaaaaata
     1321 aatcgacttt taccatcaat tttattacgt attcattgat gctttctctg gcaggagtgc
     1381 ctgtctatgc cgtcgatttt aatacggatg ttctggatgc cgcagacaga cagaatattg
     1441 atttttcccg gttctcccgg gccggctata ttatgcccgg acagtatcag atggaaatca
     1501 gggttaatga ccaggatatt tcaccatcgg catttcagat tgcgtttctt gaaccacctt
     1561 tttcggattc ggataacgag aaaccgttac cagaaccctg cctgacaccg gagatagtca
     1621 gccggatggg actgactgag gcgtcacagg aaaaagttac gtactggaat aacggacaat
     1681 gtgcggattt ccgacagtta tccggagtgg agatacgccc gaatccggca gaagggatgc
     1741 tgtacatcaa tatgccgcag gcctggctgg aatattccga cgcctcctgg ctgccgccgt
     1801 ctcgctggga taacggtatt ccggggctgt tgtttgacta caacatcaac gggacggtga
     1861 acaagccaca caagggaaag cagtcacagt cactgagcta taacgggact gcgggggcca
     1921 acttcggtgc ctggcgtctg cgtgcggact atcagggtaa cctgaatcac accaccggca
     1981 gcgggcaggg aacggacagt cagttcacct ggagccgttt ctacatgtac cgcgccatcc
     2041 cgcgctggcg cgcaaatctc acactgggtg agaactacat caattctgag attttcagct
     2101 cctggcgcta caccggtgcc agtctggaaa gtgatgaccg catgctgccg ccaaaactgc
     2161 gcggttatgc accgcaggtc tcagggattg cggatacgaa tgcgcgtgtc gtgatttcgc
     2221 agcagggacg catcctttat gactccaccg tgccggcagg tccgttcacc attcaggacc
     2281 tggacagctc cgtgcgtggt cgcctggatg ttgaggttat cgagcaggac gggcggaaaa
     2341 aaacgttcca ggtggacacg gcatatgtgc cgtatctgac ccgtccgggc caggttcgtt
     2401 accggctggt ttccggtcgt tcgcgtaatt atgaacacac catggaggga ccggtttttg
     2461 ctgccggtga ggcatcctgg ggtatcagta acaagtggtc gctttacggt ggcggtatcg
     2521 ttgccgggga ttacaacgcc ctggctgtgg ggctgggccg ggacctgagt gagttcggta
     2581 ctgtttccgc tgatgtaacc cagtccgtgg cccgtattcc gggagaagag acgaagcagg
     2641 ggaaatcctg gcgtctgagc tattccaaac gttttgatga cgtgaatacg gatatcactt
     2701 ttgcgggtta ccgcttctct gaacgcaatt acatgacgat ggaccagtat ctcaatgccc
     2761 gttaccggaa tgacttcacg ggacgtgaga aggagctgta caccgtcacg ctcaacaaga
     2821 acttcgagga ctggaaggca tcggtcaacc tgcagtacag ccatcagact tactgggacc
     2881 gccggacatc tgactattac acgctgtcgg tgaaccgcta cttcgatgcg ttcagcttta
     2941 agaatatcgc gctggggctg tctgcatccc gttcgaaata ccagaaccgg gataacgatt
     3001 cggcgtttgt tcgtctgtcc gtgccgtggg gaaccggtac ggccagctac agcggcagca
     3061 tgagcaacga ccgttacacc aatacggtgg gttacagcga cacactgaat aacggcctga
     3121 gcagttacag cctgaatgcc ggcgtgaaca gtggcggtgg tcagccgtca caacggcaaa
     3181 tgagtgccta ttacaaccat aacggttccc tgacaaatct gtcagccagt ttctctgccg
     3241 tggagaacgg atacagctca ttcgggatga gtgcatcggg gggggcgacg gtcacgatga
     3301 aaggagctgc gttacatgcc ggaggcatga acggaggaac ccgtcttctg gtggataccg
     3361 atggtgtcgg aggtgtgccg gttgacggtg gccgggttta taccaaccgg tggggcattg
     3421 gtgtggtgac ggatgtgagc agctattacc gcaacaccac ctcagtggac ctgaacaggc
     3481 tgccggaaga catggaagcc acccgctcgg tggtggaatc ggtcctgacg gaaggggcca
     3541 ttggttacag ggagtttgaa gttctgaaag gtagccgtct gtttgctgtg ctgcgtctgg
     3601 cagacaacag ccatccgccg tttggtgcca gcgtgacaaa cgcgaaaggc cgtgagctgg
     3661 ggatggtggc agacagcggt ctggcctggc tgagcggggt gaatccgggt gaaaccctga
     3721 atgtcggctg ggacggccgg acacagtgtg tggtggacat cccggcacat ccggacccgg
     3781 cacaacagtt gctgctgccc tgtcgtcagg tgaagtgacg ttatacacgc cggcgatcgt
     3841 ctgtatatcc cggcagaaaa atgtaaagaa acagagagca gaaaatatgc agaaaatgaa
     3901 accggcgctg aaaaaaacgc tgatggcggt agcgtgtctg agcgcagtac ctgcagccca
     3961 agctgccatc tccctggacc gcacacgtgc gatttttaat ggcgatgaga aatcgatgac
     4021 actgaatatc gccaatgata acaaacagct tccctatctg gcccaggcat gggtggagaa
     4081 tgaaaagaaa gaaaaaatca ccacaggccc gattattgcc actccgccag tacagcgtct
     4141 ggaacccggc tcaaagagta tggttcgtct gaccagtaca cctgacatca gccgcctgcc
     4201 acaggaccgg gaatctctgt tctatttcaa ccttcgtgaa attcctccga agagtgataa
     4261 ggcaaatgtg ttgcagatag ccctgcagac aaaaataaaa ctgttttatc ggcctgaatc
     4321 tattaaagca aaacccaacg cagtatggca ggaccagctg gtgctgaata agaccggtgg
     4381 agggtatcag attgataatc cgacaccgta ttatgtgacg gtaattggaa ttggcggcag
     4441 tgaaaaacag gccagagaag gtgaatttga tgctgtgatg ctggcaccaa agtcaacgca
     4501 gatggtgaaa tcaggaacat acagcacacc ctatctgagt tatatcaatg actatggtgg
     4561 acgtccggta ctgcagttct cctgtgcagg aagtcgctgt actgcagtaa agaaataaga
     4621 aagcagtaaa acgaactgac cggaggtgtc ggagatgaac agggcaacaa taggatttta
     4681 tctggcagtt gtgctgggga gttgcagtat gaacggtgtc tcacaggctg atgaattgtt
     4741 gacgtgggat gattttttcg tagcagatga aagccgccat cagtgggtaa atgagcacaa
     4801 cggaaggact ggagcgctga atgtcaaagg ggcattagtg tcatctccgt gtcagctgga
     4861 tactaatgag gtgcaaattt cagaacggaa gaaacgacgt gatatacaga aagttaagat
     4921 caatatgtca ggttgtggtt atggtaatgt tccggagtcg ttctcagaat ggaacagaaa
     4981 attatatgta aatgcatcaa ttataatgtc tgatttactg agaagagcat gttatcttcc
     5041 ttcaaaaaga aagtttttgt ctgatggtga gaaccagttg atattttatt tggaatgtgg
     5101 gacgcatatc cgaagtcctg agaaagtcgg cagagaggaa aatgctctta tcaggatgtc
     5161 atatgattaa atgaaaaatg atataataat attgatcggg tatgatgagt atgttaagga
     5221 aatttatatt tttgtttgtt ttatttactt tcgtaaattc agtgcgtgca gttgacgtgc
     5281 ctgtgagaat atatggaaca attattatac ctccttgtga aataaactca ggagagcctg
     5341 tgaatgttga ttttgggaat gttcaggaag agaagataaa ctctcgcaca tatgataaga
     5401 aaattattgt cccggtcagg tgcccttatc atcaggggga tgtatcgtta acaattacag
     5461 ctgcttccat aattgaaaat gcagatgttg ttgctactga tattgaaggg ttggggattt
     5521 tgttgtatga agaagggaat aataaacctt tgtcattaaa taatgcagcc acaataagca
     5581 caggtttacg cgggaaa
//
LOCUS       DBBGLQ010000194         5570 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.194, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000194 DBBGLQ010000000
VERSION     DBBGLQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.194"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            17..1066
                     /gene="sohB"
                     /locus_tag="ACIYSB_005120"
     CDS             17..1066
                     /gene="sohB"
                     /locus_tag="ACIYSB_005120"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGC5431964.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(1102..1353)
                     /gene="yciN"
                     /locus_tag="ACIYSB_005121"
     CDS             complement(1102..1353)
                     /gene="yciN"
                     /locus_tag="ACIYSB_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGC5431965.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            1733..4330
                     /gene="topA"
                     /locus_tag="ACIYSB_005122"
     CDS             1733..4330
                     /gene="topA"
                     /locus_tag="ACIYSB_005122"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGC5431966.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4540..5514
                     /gene="cysB"
                     /locus_tag="ACIYSB_005123"
     CDS             4540..5514
                     /gene="cysB"
                     /locus_tag="ACIYSB_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGC5431967.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         1481 a         1285 c         1478 g         1326 t
ORIGIN      
        1 accaaggtgg ggactcgtgg aattgttgtc tgaatatggt ttgtttttgg cgaaaatcgt
       61 taccgttgtg ctagcgattg cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa
      121 taaacgccag cgtggcgagt tacgggtcaa caatctcagc gaacagtata aggagatgaa
      181 agaagaactg gccgcggcgc tgatggactc acatcagcaa aaacagtggc acaaagcgca
      241 gaagaaaaag cataagcaag aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt
      301 ggcaactgac agtaaacccc gcgtctgggt gctggatttt aaaggcagca tggacgccca
      361 tgaagtgaac tcgctacgtg aagagataac ggctgtactc gcagcattca aaccgcagga
      421 tcaggttgtg ctccgtctgg aaagccctgg tggcatggtg catggttacg ggctggcggc
      481 ttcgcaactg cagcgtctgc gcgataaaaa cattccttta actgttacgg tagacaaagt
      541 cgctgccagc ggcggttaca tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt
      601 tgctattgtg ggttccattg gcgtggtggc gcaaatgccc aactttaacc gcttcctgaa
      661 aagcaaagat attgatatcg aactgcacac tgccgggcag tataagcgca cgctgacgtt
      721 gctgggtgaa aataccgaag aagggcggga gaaattccgc gaagagttga acgaaacgca
      781 tcagttgttt aaagattttg tgaagcgtat gcgtccttct ctggatattg aacaggtggc
      841 aacgggtgaa cactggtacg gacaacaggc ggtagagaaa ggcctggttg atgaaatcaa
      901 caccagtgat gaagttattc ttagcctgat ggaaggccgt gaagtggtca atgtacgcta
      961 tatgcagcgt aaacgactca ttgaccgatt caccggcagc gcggcagaga gcgccgatcg
     1021 attgttgcta cgctggtggc agcggggtca aaagccattg atgtaaaaga caaacgcgag
     1081 gctaagacct cgcgttttgc tttaatcaac cagatgatat ttttctgaaa gcacatgggc
     1141 caggtgtttg aacatattaa acaccgcggt gcttttggct gttggcaatc cttgttcatc
     1201 taaaaagtag tcgccggtaa ataccagcac gccattacgc tgcgtgacgc cggtggcttc
     1261 aatccctgcg agcgtatcct catgctcacg aatgattttg ttggcctctt tcaacagcgt
     1321 ttcgcggtcg atgggttgtg tctctttgtt cattatttac tccttaaaca aggacattag
     1381 tctacgccag gcatggcttg cagacaaata taccacgctg gtggcaagag cgccttactg
     1441 gcaactttgg attttgcatg ctaataaagt tgcgtatcgg attttatcag gtacagtgtg
     1501 acgctttcgt caatctggca atagatttgc ttgacattcg accaaaattc cgtcgtgcta
     1561 tagcgcctgt aggccaagac ctgttaactc agtcacctga attttcgtga acagagtcac
     1621 gacaaggggt tgatatccgc agagagcgag tccatatcgg taactcgttg ccagtggaag
     1681 gtttatcaac gtgcgacgca ttcctggaag aatcaaatta ggtaaggtga atatgggtaa
     1741 agctcttgtc atcgttgagt ccccggcaaa agccaaaacg atcaacaagt atctgggtag
     1801 tgactacgtg gtgaaatcca gcgtcggtca catccgcgat ttgccgacca gtggctcagc
     1861 tgccaaaaag agtgccgact ctacctccac caagacggct aaaaagccta aaaaggatga
     1921 acgtggcgct ctcgtcaacc gtatgggggt tgacccgtgg cacaattggg aggcgcacta
     1981 tgaagtgttg cctggtaaag agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc
     2041 cgaccacatc tatctcgcaa ccgaccttga ccgcgaaggg gaagccattg catggcacct
     2101 gcgggaagtg attgggggtg atgatgcgcg ctatagccga gtggtgttta acgaaattac
     2161 taaaaacgcg atccgccagg catttaacaa accgggtgag ctgaatattg atcgtgttaa
     2221 tgcccagcag gcgcgtcgct ttatggaccg cgtggtgggg tatatggttt cgccgctgct
     2281 atggaaaaag atcgctcgtg gtctgtctgc cggtcgtgtg cagtcggtgg cggttcgcct
     2341 ggtggtcgag cgtgagcgtg aaattaaagc gttcgtgccg gaagagttct gggaagtcga
     2401 tgccagcacg accacgccat ctggtgaagc gttggcgtta caggtgactc atcagaacga
     2461 caaaccgttc cgtccggtca acaaagaaca aactcaggct gcggtaagtc tgctggaaaa
     2521 agcgcgctac agcgtgctgg aacgtgaaga caaaccgaca accagtaaac ctggcgctcc
     2581 ttttattacc tctacgctgc aacaagctgc cagcacccgt cttggatttg gcgtgaaaaa
     2641 aaccatgatg atggcgcagc gtttgtatga agcaggctat atcacttaca tgcgtaccga
     2701 ctccactaac ctgagtcagg acgcggtaaa tatggttcgc ggttatatca gcgataattt
     2761 tggtaagaaa tatctgccgg aaagtccgaa tcagtacgcc agcaaagaaa actcacagga
     2821 agcgcacgaa gcgattcgtc cttctgacgt caatgtgatg gcggaatcgc tgaaggatat
     2881 ggaagcagat gcgcagaaac tgtaccagtt aatctggcgt cagttcgttg cctgccagat
     2941 gaccccagcg aaatatgact ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa
     3001 agcacgcggt cgtattttgc gttttgatgg ctggacaaaa gtgatgcctg cgttgcgtaa
     3061 aggcgatgaa gatcgcatct taccagcagt taataaaggc gatgctctga cgctcgttga
     3121 acttacacca gcccagcact ttaccaagcc gccagcccgt ttcagtgaag catcgctggt
     3181 taaagagctg gaaaaacgcg gtatcggtcg tccgtctacc tatgcgtcga tcatttcgac
     3241 cattcaggat cgtggctacg tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg
     3301 cgaaatcgtc accgatcgcc ttgaagaaaa tttccgcgag ttaatgaact acgactttac
     3361 cgcgcagatg gaaaacagcc tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt
     3421 actggatcac ttcttctcgg atttcaccca gcagttagat aaagctgaaa aagatccgga
     3481 agagggtggt atgcgcccga accagatggt tctgaccagc attgactgcc cgacttgtgg
     3541 tcgcaaaatg gggattcgca cagcgagcac cggggtattc cttggctgtt ctggctatgc
     3601 gctgccgccg aaagagcgtt gcaaaaccac cattaacctg gtgccggaaa acgaagtgct
     3661 gaacgtgctg gaaggcgaag atgctgaaac caacgcgctg cgcgcaaaac gtcgttgccc
     3721 gaaatgcggc acggcgatgg acagctatct catcgatccg aaacgtaagt tgcatgtctg
     3781 tggtaataac ccaacctgcg acggttacga gatcgaagag ggcgaattcc gcattaaagg
     3841 ttatgacggc ccgatcgttg agtgtgaaaa atgtggctct gaaatgcacc tgaaaatggg
     3901 gcgtttcggt aaatacatgg cctgcaccaa cgaagagtgt aaaaacacac gtaagatttt
     3961 acgtaacggc gaagtggcac caccgaaaga agatccggtg ccattacctg agctgccgtg
     4021 cgaaaaatca gatgcttatt tcgtgctgcg tgacggtgct gccggtgtgt tcctggctgc
     4081 caacactttc ccgaaatcgc gtgaaacgcg tgcgccactg gtggaagagc tttatcgctt
     4141 ccgcgaccgt ctgccggaaa aactgcgtta tctggccgat gcgccacagc aggatccgga
     4201 aggtaataag accatggttc gctttagccg taaaaccaaa cagcaatatg tctcttcgga
     4261 aaaagacgga aaggcgactg gctggtcagc attttatgtt gatggcaaat gggttgaagg
     4321 aaaaaaataa cctttaattc tgtcaggttt ttataaacaa agggtcgcga aagcggccct
     4381 tttttattgc atattatttt ttcttcacac ctatacacta aggctataaa tgatatagtg
     4441 gttatagtta gcaccttttt tattattaaa tcgtattagt cacccgccag gtgtgacgaa
     4501 aaaacgatgt tctgatggcg tctaagtgga tggtttaaca tgaaattaca acaacttcgc
     4561 tatattgttg aggtggtcaa tcataacctg aatgtctcat caacagcgga aggactttac
     4621 acatcacaac ccgggatcag taaacaagtc agaatgctgg aagacgagct aggcattcaa
     4681 attttttccc gaagcggcaa gcacctgacg caggtaacgc cagcagggca agaaataatt
     4741 cgtatcgctc gcgaagtcct gtcgaaagtc gatgccataa aatcggttgc cggagagcac
     4801 acctggccgg ataaaggttc actgtatatc gccaccacgc atacccaggc acgctacgca
     4861 ttaccaaacg tcatcaaagg ctttattgag cgttatcctc gcgtttcttt gcatatgcac
     4921 cagggctcgc cgacacaaat tgctgatgcc gtctctaaag gcaatgctga tttcgctatc
     4981 gccacagaag cgctgcatct gtatgaagat ttagtgatgt taccgtgcta ccactggaat
     5041 cgggctattg tagtcactcc ggatcacccg ctggcaggca aaaaagccat taccattgaa
     5101 gaactggcgc aatatccgtt ggtgacatat accttcggct ttaccggacg ttcagaactg
     5161 gatactgcct ttaatcgcgc agggttaacg ccgcgtatcg ttttcacggc aacggatgct
     5221 gacgtcatta aaacttacgt ccggttaggg ctgggggtag gggtcattgc cagcatggcg
     5281 gtggatccgg tcgccgatcc cgaccttgtg cgtgttgatg ctcacgatat cttcagccac
     5341 agtacaacca aaattggttt tcgccgtagt actttcttgc gcagttatat gtatgatttc
     5401 attcagcgtt ttgcaccgca tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc
     5461 tctaatgaag aaattgaggt catgtttaaa gatataaaac tgccggaaaa ataatttctt
     5521 gcgttatttt cggcaccttt tatgtagcga aggtgccgga atatattctc
//
LOCUS       DBBGLQ010000195         5503 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.195, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000195 DBBGLQ010000000
VERSION     DBBGLQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.195"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            196..1941
                     /gene="dld"
                     /locus_tag="ACIYSB_005124"
     CDS             196..1941
                     /gene="dld"
                     /locus_tag="ACIYSB_005124"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGC5431968.1"
                     /translation="MAIFTGPDIRSGEPPSMKHTDNKTFLTTLTQLVGPAHILTEPSK
                     TARYRKGFRSGQGDALAVVIPGSLLELWKVLSACVEADKIILMQASNTGLTEGSTPNG
                     NDCDREIVIISTLRLDSLHVLDEGKQVLAYPGTTLYSLEKALKPFGREPHSVIGSSCI
                     GASVIGGICNNSGGSLVQRGPAYTEMSLFARIDEQGRLQLVNHLGIELGQTPEQILAR
                     LDNNAIEAVLVNHDGRHAHDHDYVSRVRDINADTPARYNADPDRLFESSGCAGKLAVF
                     AVRLDTFEAEKNQQVFYIGTNQTDVLTEIRRHILANFTNLPVAGEYMHRDIYDIAEKY
                     SKDTFLMIDKLGTDKMPFFFNLKGRTEAILEKVKIFRPHFTDRAMQAFSHLFPSHLPA
                     RMKTWRDNYEHHLMLKMSGEGVAEAQAWLTDYFKSAEGAFFTCTAEEGSKAFLHRFAA
                     AGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPAEIDSQLVHKLYYGHFMCHVF
                     HQDYIVKKGVDAHALKEQMLELLRQRGAQYPAEHNVGHLYKAPENLARFYQENDPTNT
                     MNPGIGKTSKHKNWA"
     gene            2016..2735
                     /locus_tag="ACIYSB_005125"
     CDS             2016..2735
                     /locus_tag="ACIYSB_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGC5431969.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWALRAE
                     KVSARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLTRKLGVKEEPLTLLKNVRGL
                     ELLTFADQDTCCGFGGTFSVKMAEISGEMVKEKVLHLMDAKPEFLIGADVSCLLNIGG
                     RLQREGQPVKVMHIAEVLMSR"
     gene            2746..4173
                     /locus_tag="ACIYSB_005126"
     CDS             2746..4173
                     /locus_tag="ACIYSB_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGC5431970.1"
                     /translation="MSIKTSDVEFKLRIRQQIEDPIMRKAVANAQERIGANRQKMVDE
                     LGHWEEWRDRASQIRDHVLSNLDAYLYQLSEKVTENGGHVYFAKTKEDATRYILQVAQ
                     SKNAQKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRYQIRRVLNERLGYDGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PRDADNVDGPEEFHLVIVDNGRSQVLGSEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEE
                     GITPKAERRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKVGAIGDWMEAR
                     DLPDADGESFRSWFKKHQAQEKKNG"
     gene            4166..4861
                     /locus_tag="ACIYSB_005127"
     CDS             4166..4861
                     /locus_tag="ACIYSB_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGC5431971.1"
                     /translation="MDNRTAFLNTIAQALGRPLRFEPQADAAPVNNYANERLTELSPE
                     QRCSAFIQFASEVMLARCELTSEDQAADAALRLCEELGQQPVVLSGDSRLVELGITER
                     LQQECNAVVWDPARGSENITQAEQAKVGVVYAEYGLTESGGVVLFSAPERGRSLSLLP
                     ESSIFVLRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(4911..5324)
                     /locus_tag="ACIYSB_005128"
                     /pseudo
     CDS             complement(4911..5324)
                     /locus_tag="ACIYSB_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
BASE COUNT         1287 a         1391 c         1593 g         1232 t
ORIGIN      
        1 atcattaata ggtatgaaat ggtctttgca ataaatgaaa taagcgtttt ggcatccagt
       61 aaaagaacgg agtatgaatt atgtggtagt ggtaatttca ttaaaaaatg tgagcggtgc
      121 cgaaagaaat aaaatggggt attgcatgtg gatattgatt gagggaatat tctgttttgt
      181 cgtacaaatg aacttatggc cattttcact ggccctgata taaggagcgg agagccgccc
      241 tccatgaaac atactgataa taagaccttt ttgacaactc ttacccaatt ggttggccct
      301 gctcatatcc tcaccgaacc gtcaaaaact gcgcgttacc gtaagggttt tcgctcgggt
      361 cagggtgatg ctctggcggt ggttatcccc ggctctttgc ttgaactctg gaaagtcctt
      421 agcgcctgcg ttgaggccga taaaataatc ctgatgcagg cgtcgaacac tggcctgact
      481 gagggttcga cgcccaatgg taatgattgc gaccgcgaga tcgtcattat cagcacgctg
      541 cggctggaca gtctgcatgt gctggatgag ggtaagcagg tgctggctta tccgggcacc
      601 acgctgtatt cgctggaaaa ggcgctgaag ccgtttggcc gtgagccgca ttcggtgatt
      661 ggctcttcgt gcatcggcgc gtcggttatc ggcggtatct gtaataactc cggcggttct
      721 ctggtccagc gtggcccggc ttataccgag atgtcgctgt ttgcccgtat tgatgaacag
      781 ggacgcttgc agctggtcaa tcacctcggc attgagctag gtcagacgcc agagcaaatc
      841 cttgcccggc tggataacaa tgcgattgaa gccgtgctcg tcaaccatga cgggcgtcac
      901 gcgcacgatc acgactatgt tagccgcgtg cgggacatca atgccgacac gccagcacgt
      961 tacaacgctg acccggatcg cctgtttgaa tcctctggct gtgcgggcaa gttagcggtg
     1021 tttgccgtac gtctggatac ctttgaagcc gagaagaacc agcaggtctt ttatatcggt
     1081 accaatcaga ctgatgtcct gactgagatt cgccgccaca tcctggcgaa ttttacaaac
     1141 ctgccggttg ctggggagta tatgcaccgc gatatctatg atattgcaga gaaatacagc
     1201 aaagatacct tcctgatgat cgacaaactt ggcacggaca aaatgccgtt cttcttcaac
     1261 ctgaaggggc gtaccgaggc catacttgag aaggtgaaga tctttcgccc gcactttacc
     1321 gaccgcgcga tgcaggcttt tagccatctg ttcccgagcc atctcccggc gcggatgaag
     1381 acctggcgcg ataactatga acaccatctg atgttgaaaa tgtccggtga gggtgtggcc
     1441 gaagcccagg catggttaac ggactatttt aaatcggccg agggcgcgtt ctttacctgt
     1501 accgcggaag agggcagtaa ggcgtttctg catcgttttg ccgccgcggg tgctgccatt
     1561 cgttatcagg ccgttcattc tgacgaggtt gaggatattc tcgcgctgga tattgcatta
     1621 cgccgcaatg ataccgaatg gtatgagcat ttaccggcgg aaattgacag tcagttggtg
     1681 cataaactct attacggcca ttttatgtgc catgtattcc atcaggatta tatcgttaaa
     1741 aaaggcgtag atgcccacgc gttaaaagaa cagatgctgg aattactgcg ccaacgcgga
     1801 gcgcaatatc cggcggaaca caatgtcggc catctgtata aagcgccgga aaatttggca
     1861 cgtttttatc aggaaaatga tccgaccaac actatgaatc caggtattgg taaaaccagc
     1921 aaacataaaa actgggcatg aaatacgaat gaacatttgt ttgatatcgc gatatcaagc
     1981 gcaataaaca gaaatgaagc aatggagtag ttattgtgaa tgtaaatttc tttgtcacct
     2041 gtattggtga cgcactgaaa tcacggatgg cacgagactc cgtattgctg ctggaaaaac
     2101 tgggttgtcg cgttaatttc cccgagaagc aagggtgttg cggtcagcca gccatcaaca
     2161 gcggttacat caaagaggcg atccccggga tgaaaaacct gatcgccgcg ctggaagaca
     2221 acgacgatcc gattatctcc ccggcgggct cctgcaccta cgcggttaaa agctacccga
     2281 cgtatctggc cgatgaaccg gaatgggcgc tgcgtgctga gaaagtttcc gcgcgtatgc
     2341 aggatctcac ctcctttatc gtcaacaaac tgggtgtggt ggacgttggt gccagcctgc
     2401 agggccgcgc tgtgtatcat ccttcctgta gcctgacccg caaactggga gtgaaggaag
     2461 agccgcttac gctgctgaaa aacgtgcgcg gactggagct gttgacgttt gccgatcagg
     2521 atacctgttg cggctttggc gggacgttct cggtcaagat ggccgagata tccggtgaga
     2581 tggtgaaaga gaaagtatta catctgatgg acgcaaagcc ggaatttttg attggcgcag
     2641 acgttagctg cctgctgaac attggcggtc gactgcaacg ggaaggccag ccggtcaaag
     2701 tgatgcatat tgctgaagtg ctgatgagcc gctgagggga aagagatgtc gataaaaacc
     2761 agcgatgtag aatttaaact gcgtattcgt cagcagatag aagatccgat tatgcgcaaa
     2821 gccgtggcta atgcgcagga gcgtatcggc gcgaatcggc aaaaaatggt cgatgagtta
     2881 ggccactggg aagaatggcg cgatcgtgcc agccagatcc gcgatcacgt tctgagcaac
     2941 ctcgacgctt acctgtacca actctccgag aaagtgacgg aaaacggcgg acacgtctat
     3001 ttcgccaaaa ccaaagaaga tgccactcgc tacattttgc aggtcgcgca gagtaaaaat
     3061 gcgcagaagg tggtgaagtc caagtcgatg gtgaccgaag aaatcggcgt caaccatgtg
     3121 ttgcaggatg ccggaatcca ggtgatcgaa accgatctcg gggaatatat cctgcagttg
     3181 gatcaggatc cgccttcgca cgtcgtggtt ccggcgattc ataaagatcg ctaccagatc
     3241 cgccgcgtgc tgaacgaacg tctggggtac gacggcccgg aaaccccgga agctatgacc
     3301 ttgttcattc gtcagaagat ccgcgaggat ttcctcagtg ccgaaatcgg tattactggc
     3361 tgtaactttg ccgtggcgga aaccggctcg gtgtgtctgg tgaccaacga aggcaacgcg
     3421 cgcatgtgca ccacgctacc gaaaacgcac attgcggtga tgggcatgga gcgtatcgcg
     3481 ccgacctttg ccgaggtgga tgtgctgatt accatgctgg cgcgcagcgc cgtgggcgca
     3541 cgtctgacgg gctacaacac ctggctgacc ggcccgcgtg atgcggacaa cgttgacgga
     3601 ccggaagagt tccatctggt gattgtcgat aacggtcgct cgcaggtgct gggctcagag
     3661 tttcgcgatg tgctgcgctg catccgctgc ggagcctgta tgaacacctg tccggcgtac
     3721 cgccacatcg gcgggcacgg ctatggctct atctatcccg gccctatcgg cgcggtgatt
     3781 tctccgctgc tcggcggcta taaagatttt aaagacctgc cgtacgcctg ttctttgtgc
     3841 acggcttgtg acagcgtctg cccggtgcgt attccgttgt caaaactgat cctgcgtcat
     3901 cgccgggtga tggccgagga gggcattacg ccgaaagccg agcgacgggc gatcaagatg
     3961 ttcgcctatg ccaatagcca tccggggctg tggaaagtcg ggatgatggc gggcgcgcat
     4021 gcggcgagct ggtttatcaa tggcggcaaa accccgctca aggttggcgc gattggtgac
     4081 tggatggagg cgcgcgatct gcctgatgct gacggcgaga gcttccgcag ctggtttaag
     4141 aaacatcagg cgcaggagaa aaagaatgga taaccgtacc gcgtttttaa acaccatcgc
     4201 ccaggcgctg ggtcgcccgc tgcgcttcga accgcaagcg gatgccgcac cggttaacaa
     4261 ctatgccaac gagcgcctga ccgagcttag ccccgagcag cgctgctctg cgtttatcca
     4321 gtttgccagc gaagtgatgc tggcccgctg cgaactgacc agcgaggacc aggccgcaga
     4381 cgcggcactg cgcctgtgcg aagaattggg tcagcagccg gtggtgctga gcggcgacag
     4441 ccggctggtg gagctgggca tcaccgaacg cttgcagcag gaatgcaacg ccgtggtctg
     4501 ggatccggcg cgtggtagcg aaaatattac ccaggccgag caggcgaaag tcggcgtggt
     4561 gtatgccgaa tatggcctga ccgagtccgg cggcgtggtg ctgttttccg caccggagcg
     4621 cgggcgctcc ctgagcctgt tgccggagtc ctctattttc gtgctgcgta aaagcaccat
     4681 cctgccgcgc gtggcgcagc tggcagaaaa actgcatcag aaagcacagg ccggtgagcg
     4741 tatgccatcg tgcattaata tcatcagtgg acccagctcg acggcggata ttgagcttat
     4801 caaggtggtt ggggtgcatg gtccggtgaa ggcggtatat ctgattattg aggattgttg
     4861 aggcaggatg gaaaaccgct ctggcgctgt gggccagagc ggtatcaacg ttatgcaatc
     4921 atgctgatga tcaacacgat tatcaggcca accacggagt ttaccagctc cagcagaccc
     4981 caggttttca gcgtatcctt caccgacaga tcaaagtagc ctttgaacag ccagaacgaa
     5041 gcatcgttga tatgggtcag cgtgttggag ccagccgctg ttgccagcac cagcaacgcc
     5101 gggtcgacgc ccaccagttg accggttgcc gggtcgagga ttgctgcgct aataatcccc
     5161 gccgccgtca tggctgagac cacgccctga cccgttgcca gacggatcag cacggttatc
     5221 aaccacgcca tgatgtaggg agaaatattg ccgtgtgaca tcagcatgcc gatggtatcg
     5281 ccgatgccgg tatcgataat ggtttgcttc agtacgccgc ctgccactga ctgccggagc
     5341 agggactgcg ctgactctct aaatccgccg ctctgtacgt tgggattgtt aatccgagta
     5401 actaagtaac acgctatacc ttgactcctt atgcttacat gcttgcacat cgttacggca
     5461 caaaattcaa ctgtggcaaa gcaagtgcgc tgttgcgttc tct
//
LOCUS       DBBGLQ010000196         5438 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.196, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000196 DBBGLQ010000000
VERSION     DBBGLQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.196"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..418)
                     /locus_tag="ACIYSB_005129"
     CDS             complement(<1..418)
                     /locus_tag="ACIYSB_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5431972.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKK"
     gene            complement(560..829)
                     /locus_tag="ACIYSB_005130"
     CDS             complement(560..829)
                     /locus_tag="ACIYSB_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGC5431973.1"
                     /translation="MKELELKKPIIAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(829..1185)
                     /locus_tag="ACIYSB_005131"
     CDS             complement(829..1185)
                     /locus_tag="ACIYSB_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGC5431974.1"
                     /translation="MSRIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVNKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(1185..2681)
                     /locus_tag="ACIYSB_005132"
     CDS             complement(1185..2681)
                     /locus_tag="ACIYSB_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGC5431975.1"
                     /translation="MTISFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATV
                     TLTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPTLPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGSPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGSLSELVTVG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGDLVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(2665..2835)
                     /locus_tag="ACIYSB_005133"
     CDS             complement(2665..2835)
                     /locus_tag="ACIYSB_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HGC5431976.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(2844..3404)
                     /locus_tag="ACIYSB_005134"
     CDS             complement(2844..3404)
                     /locus_tag="ACIYSB_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431977.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEITLPS"
     gene            complement(3401..3907)
                     /locus_tag="ACIYSB_005135"
     CDS             complement(3401..3907)
                     /locus_tag="ACIYSB_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431978.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGVSGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(3882..4292)
                     /locus_tag="ACIYSB_005136"
     CDS             complement(3882..4292)
                     /locus_tag="ACIYSB_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGC5431979.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGSNGDSLFSR"
     gene            complement(4289..4612)
                     /locus_tag="ACIYSB_005137"
     CDS             complement(4289..4612)
                     /locus_tag="ACIYSB_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGC5431980.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(4615..4815)
                     /locus_tag="ACIYSB_005138"
     CDS             complement(4615..4815)
                     /locus_tag="ACIYSB_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431981.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGISAKSSKAAKEKKATTSKEG"
     gene            complement(4864..>5438)
                     /locus_tag="ACIYSB_005139"
     CDS             complement(4864..>5438)
                     /locus_tag="ACIYSB_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGC5431982.1"
                     /translation="NSELATEFAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFG
                     KLQHIVSGEATAVTADAIIKLIYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWR
                     PGLELGQPSSLAGYGIAENEQMPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTN
                     KPFVGFYTTKRTGGMLVDSQAIKLLKIAAA"
BASE COUNT         1150 a         1630 c         1399 g         1259 t
ORIGIN      
        1 tttttttcag ttgtaccgca agataagctg tcgctttacc cacaccaccc agaatgtttt
       61 ctgccgggat cccctgacgc accagcatct gcatcatgtt ctggaaatca gccgttgtac
      121 cgggtagctg gttacccagg ccaatagcca gtttattgat gtcctgaaag ctctttccaa
      181 cctcgccgtt cgcatccatc atggcgactt tcagcccggt ggcggcgttt tcctgatcgg
      241 cataagattt cagggaaagc gtcagacccg ctgccagtcc gccaccaagc gccagcccac
      301 cctgtgacgc ttcttccgcc tggcgtttaa atccccggat tttcttttgc attttcgaca
      361 gcgcgggaga aagcctgtcg acaccggtga tcaacgcctt aagctcaaat tcagccatgt
      421 gtgcgtttct cctgctctat cctgtttgcc tgactgacca gtaagggaat ttcactgatc
      481 ggcatattca gcaattcgaa aggattaatg cgccagtaac tggcgcagtc aaagaagcga
      541 tcagtgaggt attcagccgt caggcctgga ggaaaaaacc ggccacaagc cacgccgctg
      601 cattcagatc tgccggagac atctggtcga cagagctttg cggcactttc gccagccgca
      661 cgatgtattt cgataccaca tgcgccagaa gtctgacgga ctcatcctga ttcatctggt
      721 agggatatcc cagctcgcgg acatctttcc cggtgggctc atcaaactcc agtacggaga
      781 gtgtctcacc atgagcgata atcggtttct ttaactcaag ctctttcatt actggtaatc
      841 cccttcttca ccgtggaact caagatcaac cgtgccttct tcggcattat ggttcgcttc
      901 gccgtgcagc caggcagacg acagtacata gacctgaccg ttcgccagct cggcagtgat
      961 tgtcatctca tcagacgagg tgattttgtt caccggaaaa ttcttcggca ccttgaaggt
     1021 ccctttgaca taaggcgcac ggtgagtttc cttgcggtcc actgagccgt ccaggccgat
     1081 gatgtcatca ttgaccgtcc tgttcatggg cacctcaatg ccgccggtca gcgatagctg
     1141 ctgaccgtca attttgaaat aacaggttcc cccgatacgg gacattatgc agactcctct
     1201 gaatactgaa gacggaactg gttaaccacg gcaaagacac gcaactggtt aacatagtca
     1261 ggcgggaaca gcgtgttcag gcggttcgga tcgctggcat cacgctccac aaccaggtac
     1321 tgcttgaaca gttcgtagtt ttccacgatc cccgcacgct cgagctgacg gtaggttgcc
     1381 agcagttccc ctttgatcac cgccggggtg acaatcgcct gaccgggacc aaagcgggta
     1441 ccgtcactgg caagcttgtg acgcccgtac ttactggtaa tgacggattt cagtttgcgc
     1501 agtacatacg cgctggtatg cagcgtctcg ctgtcgaggt agctgttatc cgcaaccccg
     1561 taagcgtttt tcctgtacgt ggtgacatca cgctgaatgc gcagtacccc gctttcgaca
     1621 tacgccgttg ccacgccatg agacagcagg gtctgttgtt cggtcatcgt gaaccgtttc
     1681 cccttcggcg caggcagcat acccaccaga tcaccggtct gcgtgggacg tgccggatcg
     1741 ttgcgaataa acaccgctgc gcgggcggta cggcttgccg ccagctcgtc ggcaggcgtc
     1801 tgggtctctt tttcgtaccc cgccagggta atgtgctgct ggttaaactg gtcacctacg
     1861 gtcaccagtt ctgacagcga gccgatcttt gccgtataca catgaccata cagctgacgc
     1921 gcatagctcc agcgaccgct ggtatcgttc atctcggtca ccagcgtgtt aacggaggcc
     1981 gtgtcgttga acggcagacc gatataatca aacggctcat ccgccattgc agccaccgca
     2041 ccggtgagaa caggagagcc cgttccagcg gtccccgtcg ccacggcaat ctgtacgccc
     2101 gcaggcagca cttctccccc accgaagccg tagtaattga ggctgacagg aatttcattc
     2161 ccgcaaagcc ccttatgacg cgcggtcagt gtgaccacgc cagccgaaga tgaagctgta
     2221 aacggcagag tcggaacggc attgatggca tcctggatac tgctggcaat cgtcgtgacg
     2281 ttatcgccgt tggtcaccgg agcctgcacg cgggtacgtc ccacatagac attcaccgtg
     2341 ccgctttcgg ttgcttcccc ggtcaccgtc agcgtaaccg ttgccgccgc gcctgtggct
     2401 tccggaacgg caatcacata cagctcacca aacgggtcgg tctggcgata agcctcgacc
     2461 atacgcgcca gctgacttcc cgcaccacaa atctggcgtg catagtctgc cgatggcatc
     2521 agcaccagac tgttggcaac aatctctgca ccgttattgg catgaccaat cagcagcgat
     2581 gctccgctgt cctgtgcagt attcgccgcc gagttatcca tttccgcata aaaaatcgga
     2641 accagcgtat tcgacggaat ggtgttaaag cttatcgtca tcggtattca cctttttatt
     2701 cacgcgccgg atatcacccg ctgcttcacg gcgcagccag tagttgttct catcaacatt
     2761 tcgcccctcg gcgggcaaaa ggtcgccgcg ggcagggtca ggaactgacc gccctttaac
     2821 aggtttcaca aacatgaaga ttctcaggaa ggaagggtta tttcggtgtg atgttcgata
     2881 tcgccgtcag gcccgttacc gggatcgaga taatcaacat caatcgccag cgttcgcagt
     2941 tcatccagac tgttcaggtc atcctgctgg cgggtatcgt cttcggtcag ctcgctgatg
     3001 accgaaaaat cgaactgata aatcagctca tgacgattca gatccagcag cgtgccgccg
     3061 tcataggtaa tcgggttacc gcacgcttcc gggttccagc ccagcagggc cttaaagagc
     3121 atctgccgga catcgtccac cacatcatac gaggcaaact gaccgcgctc atcacgcccg
     3181 ttactcagta tgacaaccac ggagaagccc tctttcagct cctgccagta gtcggtctgg
     3241 cttttgtttt ctcccggaga gtcatcaccc ggtaccacat acgccgccgg gagtctcagc
     3301 tttccgacct ccggcagatt tttgaactgt gccgcgcctg ccacccggtt ttcaaaatac
     3361 gggcagcggg cacgcagcgc agcaataaca ggcgtcagtt tcatctgtgt cgtcgctccg
     3421 gcttcagtga tttacgcaat tcccgcgcca gaaaatagcg tgtccagctg cggttctttt
     3481 caagagtttc caccatgaag ttattacgtg gagccagtcg ccagccgctg ccaccggata
     3541 caccacgatg atgactacga cgacgttttg ctcctccccg gacaccaaaa aacagaaacg
     3601 ccggatagaa gtcaccagag atcatccggt tccccttccc gttgcgctgg ttaggggcaa
     3661 tgcgtgtcat aaaaccggct cgctttttac tggctctcgg caccatataa ccaatcgaac
     3721 gagccaggcg tccggtctga taaccggggt tttcacccgg tgccgaccgc gcacggcgca
     3781 tcaccagccg acgggcatca cgcatatgac gctgcccaat cgtgacaaac gcccgccgga
     3841 cacgggcgcg gttaaagcgc atctcggcgg gctgctgaac atcaacgtga aaaaagggag
     3901 tcgccattgc tgcctccgtg actctgccta cattcgccca gctccgtaca ctccagcagc
     3961 agaaagcgcc gcgccccgtt cagatcgcgc tgacgtttca cccggtacac actgtcaccg
     4021 cagaccacct cataatcagc ggtgatcccc cggcggtaac gaatggtgat gtaatgggtg
     4081 atggcgtccc cggtctgcgc ggtttcctgc caggtggtgg cactggtctg gataaccttc
     4141 gcccatgtcc ggaacgtaac cgggtattga ggctccacgc caaagttatc cgcgggcata
     4201 tccacccgca ggcggatcag gacgcgttta ttcagttcac cggggtccgg cagaatgtag
     4261 gttgcgctgg tctgcgcctg acgaattttc attgcggaaa gtacctgtac gggccgacaa
     4321 gccagccaaa actctgcggc atgtcgagtt tctccacttc cgtaaccgac gagcggtttt
     4381 cgtaaaaatg gctgataagc atcagcatcc ccagacgaat atcatccggc aggtgcagcc
     4441 cgtccggatc gctgtccgga atggtttcat ccggtgcata gagcttccgg ttcagatacg
     4501 tttccgtccg cttttgtgcc gcacaggcca gcagttgcag atggcggtca tcagcatcga
     4561 aatcctcatc cagccggagt tgggctttaa tctcttccat tgtcagaagc atactcagcc
     4621 ctctttactg gtcgtggctt ttttctcttt tgccgcttta ctgctttttg cactgattcc
     4681 gcgctctgct aacccggcct gaagtgcaat ctcctgcacc cgggcaggaa gtgccccgtc
     4741 gtcatactca ccggcccgaa tgacctcaac acgcataccg tccggtgacc atttcagatc
     4801 ttgtttcagg atcatgattc ttcacccgtc agaacagggg cgcggttccg cgcccctgag
     4861 tgattacgcc gctgcaatct tcagcagttt gatggcctgc gaatcgacca gcatcccgcc
     4921 ggtgcgcttg gtggtataaa aaccgacaaa cggtttattg gtgtacgggt cacgcagaat
     4981 gcgggtgccg atacggtcaa cgatggtgta accccgtttg aagttaccaa atgcaatggc
     5041 tttcgcatca gcggcgatat ccggcatctg ttcgttttca gcgataccgt aacccgccag
     5101 agaggacggc tgccccagtt ccagccccgg acgccacaga tagttaccct cggtgtcttt
     5161 cagcagacgg atggcaaaca ggctgttgtt gttcatcatg aacttcgcgc cagtgcggtg
     5221 tgccttacgc agcgtgtaaa tcagtttgat aatggcgtct gcggtcaccg cggtcgcttc
     5281 gccggataca atatgctgaa gtttgccgaa cgcccggacc ttgtcggttt catcagtgga
     5341 ttcatacgcc aggaaccctt tcggcttctt ggtgccatcg cctgaggtaa aggcaatttc
     5401 ttcctgttcg gcaaattcgg ttgccagctc gctgttga
//
LOCUS       DBBGLQ010000197         5424 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.197, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000197 DBBGLQ010000000
VERSION     DBBGLQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.197"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..1699
                     /locus_tag="ACIYSB_005140"
     CDS             <1..1699
                     /locus_tag="ACIYSB_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HGC5431983.1"
                     /translation="QYIDNVSKYLGVSPNEKIDVSKPEVMTQLVRAIATKEGGNPAVN
                     NEFIKNALGAFNTNTGRWEGQFSDETLARINKIQKENGGQLIARDSQYSVGRKVKYAN
                     GKSPAQPVLNAVPTATQQPIEVAQHAQAVKKPQKTGNQPQPINPENADVNTGSPSSLL
                     ERLITINESGISGASNLFGGAATSLAGTSLDLVKDFALATSFGSISSLSEKAKGMDQA
                     LTEKISSLTGKSFGFKKASQVADIATAIQQKRTVNHDTEIIDLLPGDQVSPSTPKTPQ
                     QEQALAINQAMEGFSSAAPAPKTPQQEQALAISQAMAGFSSAAPAPKTPQQEQALAIN
                     QAMAGAAPVVTSGKRTITAPSDVQIYDNGYKVVTGEDKSFFGSLFDSTVSGLKQTGTA
                     VIPAVGDNLSRLVGGIDSTGILNDLVMQSTGQNSAIARAISPLTRNVGDWLNGGIQQT
                     ANSIRGIATGANNAIFGSASAVQEPFLAMPPQLPTVTDLARSGIRQPLTTDTINNDPA
                     MLKALDNICSILNDLLNVNKNNTKGDPDNVVKTSQPQPRPRASTTINDQSLDALLED"
     gene            1705..2079
                     /locus_tag="ACIYSB_005141"
     CDS             1705..2079
                     /locus_tag="ACIYSB_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431984.1"
                     /translation="MLYEIDARLQTNESGVIIAEGSTAAWMARLDEWLRTPEGSVYGL
                     PSWGSPMEEFKHEPFGSETSHIVEVAIEGRMMKKLRQDLPGLDVQGIRCTSISEDSLL
                     ISFYAKGGSMDIVMQKSSGVGA"
     gene            2076..3506
                     /locus_tag="ACIYSB_005142"
     CDS             2076..3506
                     /locus_tag="ACIYSB_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGC5431985.1"
                     /translation="MTITELLDKFNAKLNENTWWSRFVNSQFVQMHAIFGSQLIYIAR
                     TFASRGLTEGLISTATRRSSILAVAEDRSYVGRFVSASYGTTSITNKTDRDITLPAGA
                     ELLANDQTPLAIINSVVIPAGGTVSGVETKQHEAVSVTFDIEKETLFLTLLLSRELTK
                     EVSSLDVYVITDGVEEKWTYNPLFRMSRDKSKHYSLAYKPTEQLGVKFGDGSMGMMPP
                     AGCQVRIDVMASLGDYTLAEGQKLEPAGNIAQYVESLEFKTDSIITGGSGMETTEETR
                     NRAQYYVAYDEQVVWGGDYRQFIQNVVHGTSWLNVWGEALQEKITGFDVRNINKIFFC
                     GHKPGVSQAQLKSEILKALENVPNELNKRFEYVDTNEQPFTINFTGIARKNVLIDDAQ
                     NAIKAALEDNFGRDSSSFSLLLQSDDDSQQCYAQVKVKDIWRVIESLDMFLSYDITIQ
                     NMKDAVYFNDFIYLDVKSSTFSISYP"
     gene            3517..4362
                     /locus_tag="ACIYSB_005143"
     CDS             3517..4362
                     /locus_tag="ACIYSB_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5431986.1"
                     /translation="MKDNWLKERLTKVKQDSHLWSAFIDALQDVWNEAVEPILTRISN
                     RKSFFTMDSEDMDARIAEYGRFFVITEKDNARRPMLLAQRLDEVHFKGTILPIEQTFW
                     REFGCIPVSWEPLYAPVNIEKHPYGSYFATEIEIPTAQAQFGEFFLTSRGLVVVDQNK
                     LYRSYGEQDKEAAVQKLLSDFETVIAPLLPLHIVFDGVSFRLSAVFPEVAEILNCLST
                     DVSVIEGVYVTESMADMLSRSDTSCQVNNISLNAIPNRTAEKQLHLDVTPLDAWPLDY
                     HLQPV"
     gene            4457..4903
                     /locus_tag="ACIYSB_005144"
     CDS             4457..4903
                     /locus_tag="ACIYSB_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5431987.1"
                     /translation="MAENLKARDGNRLYKAQLLSYYYSRRAESAIGKGARFVISKAYW
                     CKSSLVTANGAGGWNIADIPLDFKLSNAQQFAVSDLILSSVDGIITINAAFPQERMPD
                     NTPYDFNTLVLVDAEEQAFAVLCTQQDTLYKGKRYSILMTIEQVEG"
     gene            4906..>5424
                     /locus_tag="ACIYSB_005145"
     CDS             4906..>5424
                     /locus_tag="ACIYSB_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5431988.1"
                     /translation="MGADKTNNIMTLSSGVSQPLLADVQYFELYSSSALNRKLKNIVL
                     PGFYCGFEPVPGTGLSVRITSENSEGKGAASVDVNNVQISVQQIEDVTVSVKAGATNI
                     IVLEANFEHGVKTTQVDSASSVSAARIYVRTDNTIGQNQIELCRVIVPSGATAVTKEM
                     IVLKYRVNRAVGV"
BASE COUNT         1607 a         1076 c         1278 g         1463 t
ORIGIN      
        1 ccaatatatt gataacgtca gtaaatatct tggtgtatcg cctaatgaaa aaatagatgt
       61 cagcaaacca gaagttatga cgcaattagt tcgtgcaatt gcgacgaaag aaggcggaaa
      121 tccggcagtt aataatgagt tcattaagaa cgcacttgga gcatttaaca caaacactgg
      181 tcgatgggag ggacaattct ctgacgaaac attggcgcgg attaataaaa ttcagaaaga
      241 aaatggtggt cagctaattg cccgcgattc gcaatatagt gttggtcgca aggtaaaata
      301 tgctaatgga aaatctccag ctcaaccagt tttgaatgcc gttccgacag caacacaaca
      361 gccaattgag gttgcacaac atgcccaggc agtcaaaaag ccacaaaaaa caggtaacca
      421 accacaaccg attaatccag agaacgctga tgttaatacc ggctctccat cgagtttgct
      481 tgagcggtta atcacaataa atgaaagcgg aatcagtggt gcaagtaatt tatttggcgg
      541 cgcggctacc agccttgctg gaacaagtct ggacctggta aaggattttg cgttagctac
      601 ttcgtttggt tctatatctt cgctttccga aaaagcgaaa ggaatggatc aggctcttac
      661 tgaaaagata tccagcttaa ccggtaaatc ttttggattt aaaaaagctt ctcaagtggc
      721 tgatatcgca acagccatac aacaaaaacg cactgttaat cacgatacag aaatcattga
      781 tctacttccg ggagatcaag tatccccttc tactccgaaa actccacagc aggagcaggc
      841 tctggcaatc aaccaggcta tggagggctt ttcgtctgca gcgccagcac cgaaaacccc
      901 acagcaggag caggctctgg caatcagcca ggctatggcg ggcttttcgt ctgcagcgcc
      961 agcaccgaaa accccacagc aggagcaggc tctagcaatc aaccaggcta tggcgggagc
     1021 tgcgccagta gttacctctg gaaaacgaac tatcacggca ccaagtgatg tgcagattta
     1081 tgacaacggc tacaaggtgg ttacaggtga ggataagagc ttctttggtt cgctatttga
     1141 ttccacggtg tcagggctta aacagacagg aaccgcagta attcctgccg ttggcgataa
     1201 cctttcacga cttgttgggg ggattgatag tacagggata ttgaacgatc ttgtaatgca
     1261 gtctactggg cagaatagtg cgattgcgcg agctattagc ccattaacaa gaaatgttgg
     1321 agactggttg aatgggggga ttcaacaaac cgctaatagc attaggggaa tagcaactgg
     1381 tgccaataac gccatatttg gctctgcttc agctgtgcaa gaaccttttc ttgctatgcc
     1441 gccacaactc cctactgtaa ccgatctcgc acgaagcgga ataagacaac cattaactac
     1501 tgacactata aataacgatc ctgctatgtt gaaggcgctg gataacatct gctctatcct
     1561 gaacgatctg ctgaatgtga acaagaacaa tacaaaaggc gatccggata atgttgtcaa
     1621 aacatcgcag ccgcagccgc gaccacgtgc gagcacaact attaatgacc agtcgctgga
     1681 tgctctgctt gaggattaat actgatgctt tacgaaatag acgcaagact tcaaacgaat
     1741 gagagcggtg tcattattgc agaaggaagt accgcagcat ggatggcccg acttgacgag
     1801 tggctacgaa cccctgaagg gagtgtttat ggtttaccct cttgggggag tccaatggaa
     1861 gaatttaagc atgaaccatt cggctctgaa acttcgcaca tagttgaagt ggctattgaa
     1921 gggagaatga tgaaaaaact acgacaagac ttgccaggtt tggatgtgca ggggattcgt
     1981 tgcacatcaa tttctgaaga ttctttatta attagttttt atgcgaaagg tgggagcatg
     2041 gatattgtta tgcagaaatc aagtggggtg ggcgcgtgac gattacagaa ttactggaca
     2101 aatttaacgc taagttaaat gaaaacacat ggtggtcacg atttgtaaat agtcagtttg
     2161 tgcagatgca cgctatattc ggatcacagc ttatttatat tgcccgtaca tttgcaagcc
     2221 gcggcctcac tgagggactt atttcaacgg ctacgcgccg ctcaagtatt ttagcggttg
     2281 ctgaagaccg tagctacgta gggcgatttg ttagtgcttc atacggaaca acgtctataa
     2341 ccaataaaac tgatcgggat attacattac cagccggggc tgaattgctt gccaatgacc
     2401 aaacaccttt ggcaattatt aacagtgttg taattcctgc tggaggaact gtttctggcg
     2461 tagaaactaa acagcatgaa gctgttagcg ttacatttga tattgagaag gaaactttat
     2521 ttctgacatt gttgctttcc agagagttaa caaaagaagt ctctagcctg gatgtttatg
     2581 ttattacaga tggcgtagaa gaaaaatgga catataaccc attatttaga atgtccagag
     2641 acaagagtaa gcactattca ttggcatata aacctacaga acaacttgga gtcaagtttg
     2701 gtgatggttc tatgggtatg atgcctccag caggctgtca ggttcgaatc gatgttatgg
     2761 ctagccttgg cgactatact ttggctgaag gacaaaagtt agaaccggct ggaaatatcg
     2821 ctcaatatgt ggagtcactt gagtttaaaa ctgattcgat cattaccggt ggtagtggta
     2881 tggaaactac agaagaaact cgaaatcgtg ctcagtatta tgttgcatac gatgaacaag
     2941 tggtatgggg cggtgattat cgccaattca ttcagaatgt tgttcatgga acttcatggt
     3001 tgaacgtttg gggcgaagcg ttacaagaga aaataactgg gtttgacgtt cgaaacatca
     3061 acaaaatttt cttttgcgga cataagccgg gtgtaagcca ggctcagcta aaatcagaaa
     3121 tactgaaagc tctagagaat gttccaaatg agttgaacaa gcggttcgag tatgtagata
     3181 caaatgaaca gccatttact ataaatttta caggtattgc acgtaaaaat gttctgatag
     3241 acgacgctca aaatgctatt aaagcagcat tggaagataa ttttggtcgt gattcgtcat
     3301 catttagttt attactacag agtgatgatg attctcagca atgttatgca caggtaaaag
     3361 ttaaagatat ttggcgagta atagaatcgc tggatatgtt tctctcttat gacataacaa
     3421 tacaaaatat gaaagatgca gtttacttta atgacttcat ttatcttgac gtgaaatctt
     3481 ccacgttcag catttcttac ccgtaatgag gttagcatga aagataattg gcttaaggaa
     3541 cgattaacta aagtaaagca ggattcacat ctctggagtg cctttataga cgctttacag
     3601 gatgtctgga atgaggcagt agaaccaata ttaacaagaa taagtaacag aaaatccttc
     3661 ttcactatgg acagtgagga tatggatgcc cgcattgctg aatacgggcg ttttttcgtt
     3721 atcactgaaa aagataatgc ccgtaggcca atgcttctgg cgcagcgttt agatgaagtg
     3781 cactttaaag gtactatctt gcctattgag cagacattct ggcgtgaatt cggttgtatt
     3841 cctgttagtt gggaaccgct gtatgcacct gtaaatatag aaaaacaccc ctatggttca
     3901 tattttgcga ccgaaataga aatacctacg gcacaggctc aattcggtga gttcttcctg
     3961 acatcaaggg ggctggtcgt tgttgatcag aacaagctat atcgctcata tggagagcag
     4021 gataaagaag ccgctgtgca gaaattgtta tctgacttcg aaacagtaat agcccctttg
     4081 ttgccattgc atattgtttt tgatggcgtc tcgtttcggc ttagtgctgt attccctgaa
     4141 gtggcagaaa tactaaattg cctgtctacg gacgtttcgg taattgaagg tgtttatgtt
     4201 actgagagta tggctgatat gctatcccgt agtgatacct cctgccaggt aaacaatatt
     4261 tctcttaacg ccataccgaa cagaacggca gaaaagcaat tacatcttga tgtaacccca
     4321 ctcgatgcgt ggcctttgga ttaccacctt caacctgttt aataattctt cccccaaatg
     4381 cgggggaaga acaacggaaa tcacaaactg agcatactct cctttggttc attgcttatg
     4441 aaggggctat gttcgtatgg ctgaaaattt aaaggcaaga gatggtaacc gcctgtataa
     4501 ggcgcaattg ctttcctact attattcacg ccgcgctgag tcggcgatcg gcaaaggtgc
     4561 gcgttttgtt atctctaagg cgtattggtg caaatctagt ctggtaactg ctaatggtgc
     4621 aggaggctgg aatatagcgg acattccact cgattttaaa ttgagtaacg ctcaacagtt
     4681 tgctgtgtcc gatctgatat tgtccagtgt ggatgggatt atcactatca atgcagcctt
     4741 ccctcaagaa agaatgccgg ataacacgcc ttacgacttt aacacgcttg tgcttgtgga
     4801 tgctgaggaa caggcttttg ctgtgctttg cacccagcaa gacaccctct ataaaggtaa
     4861 acgttacagt attctcatga caatcgagca agttgagggt tgattatggg tgctgataaa
     4921 acgaacaaca taatgacact atcctctggt gtctcacagc cgttgcttgc tgatgttcaa
     4981 tatttcgaac tctatagtag ttcggctctt aacagaaaac ttaaaaatat tgttttgccg
     5041 ggcttttact gtggatttga accggttccc ggcacagggt tgagtgtccg tataacttct
     5101 gaaaactcag aaggtaaagg ggctgcttca gtagatgtaa ataatgttca gatatccgtt
     5161 cagcaaatag aagatgtgac tgtctcggta aaggcagggg ctaccaacat tattgtgctg
     5221 gaagccaatt ttgaacatgg tgtaaaaacg acacaggtag atagcgcatc ttctgtcagt
     5281 gctgcaagaa tttacgtgcg tacggacaat actattgggc agaatcaaat tgaattatgt
     5341 cgagttatcg tgcctagcgg cgcaacggct gtgactaaag aaatgattgt gcttaaatac
     5401 cgggttaacc gtgctgttgg tgtc
//
LOCUS       DBBGLQ010000198         5421 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.198, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000198 DBBGLQ010000000
VERSION     DBBGLQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.198"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            114..218
                     /locus_tag="ACIYSB_005146"
     CDS             114..218
                     /locus_tag="ACIYSB_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431989.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            476..2674
                     /gene="speF"
                     /locus_tag="ACIYSB_005147"
     CDS             476..2674
                     /gene="speF"
                     /locus_tag="ACIYSB_005147"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HGC5431990.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2671..3990
                     /gene="potE"
                     /locus_tag="ACIYSB_005148"
     CDS             2671..3990
                     /gene="potE"
                     /locus_tag="ACIYSB_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGC5431991.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            4066..4215
                     /locus_tag="ACIYSB_005149"
                     /pseudo
     CDS             4066..4215
                     /locus_tag="ACIYSB_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            4339..4989
                     /locus_tag="ACIYSB_005150"
     CDS             4339..4989
                     /locus_tag="ACIYSB_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HGC5431992.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKDLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(5030..>5421)
                     /locus_tag="ACIYSB_005151"
     CDS             complement(5030..>5421)
                     /locus_tag="ACIYSB_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431993.1"
                     /translation="LLTPKDKTRGLTGSIAVNIAPFRCKTNCRGDNLPDNTRRWQLSG
                     GNEKELTYLHNMSAQEKVGLNPGWQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGS
                     MMHVGIIELRRCQSENCQQELKAINTH"
BASE COUNT         1373 a         1240 c         1319 g         1489 t
ORIGIN      
        1 tcatcattaa ataaattaag attgatcatt ttttattgat caccttcaca gttcaaccgt
       61 atttcctgga tagaatatct tcaccttcat tcacatcagg aaaggtaaat taaatggaaa
      121 ataacagccg cactatgccc catataaggc ggacaactca tattatgaag tttgctcatc
      181 gcaatagctt cgactttcac ttcttcaatg cccgttagtc taccgactaa gggcacttca
      241 gcgtacaggt cttcctgact ctctgtatta caggcatatg cctgtattcc gcagtgctca
      301 ttctctgtta acccttgtat tatcagccac ctgactggta agggtttctg cattctgaaa
      361 aaagcaatct acctgattta tttatcagcg gggtaacttt gcttttttcc ggcacgatcg
      421 atttctcatt cgagaaattg aggacctgct attacctaaa ataaagagat gaaaaatgtc
      481 aaaattaaaa attgcggtta gtgattcttg cccggactgt tttaccacgc agcgagaatg
      541 tatctacatt aatgaaagtc gtaatatcga tgtggcggca atagttttat cgctcaacga
      601 tgttacatgc ggaaaactcg atgaaatcga tgccacgggt tatggcatcc cggtatttat
      661 tgctactgaa aatcaagaac gtgtacccgc agagtatttg ccccgtattt cgggtgtctt
      721 tgagaattgc gaatcgcgac gagaatttta tggtcgccag ttagaaaccg ctgccagcca
      781 ttatgaaact caactgcgcc cacctttctt ccgcgcactg gtcgattatg tcaatcaagg
      841 taacagcgcg tttgattgcc ctggtcatca gggcggcgaa tttttccgtc gccatccggc
      901 ggggaatcag tttgtggaat actttggtga ggcgctgttc cgtgccgact tgtgcaacgc
      961 cgacgtagcg atgggcgatc tgctgattca cgaaggcgcg ccatgcattg cacagcaaca
     1021 tgcggcaaaa gtgtttaatg ccgataaaac ctacttcgtt ttaaatggca cttcatcttc
     1081 taacaaagtg gttttaaacg ccctgctaac accgggtgat ctggtgctgt ttgatcgcaa
     1141 taaccacaaa tctaaccacc acggagcgtt gctacaggct ggtgcaacac cggtttatct
     1201 ggaaacggca cgtaacccgt atggctttat cggtggcatt gatgcgcact gttttgaaga
     1261 aagttacctg cgtgagctga tcgcggaagt cgcaccgcag cgggcaaaag aggctcgtcc
     1321 tttccgcctc gctgtgattc agttaggcac ctacgacggt acgatttata acgcccgcca
     1381 agtggtggat aaaattggtc atctgtgtga ctacatcctg tttgactcag catgggtcgg
     1441 ctatgaacag tttattccga tgatggcgga ctgttcgccg ctgttgctgg atcttaatga
     1501 gaacgatccg ggtattctgg ttacgcaatc tgtgcataaa caacaggctg gtttttctca
     1561 gacttcacaa attcataaaa aagacagcca catcaaaggg caacagcgtt atgtaccgca
     1621 caaacgcatg aacaacgcct ttatgatgca cgcctccacc agcccgttct atccgctgtt
     1681 tgccgcactg gatatcaacg ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat
     1741 ggattgtgtg gtaaatggca ttaatgcccg caaactgatc ctcgataact gtcagcatat
     1801 tcgtccgttc gtacctgaac tggtggatgg taaaccctgg cagtcgtatg aaacagcgca
     1861 aattgcggtt gatctgcgct tcttccagtt tgtaccaggg gaacactggc attcttttga
     1921 aggctatgca gagaatcaat actttgtcga tccatgcaaa ctgttgctga caaccccagg
     1981 tattgatgca cgtaacggcg aatatgaagc gttcggtgta cccgcgacga ttcttgctaa
     2041 cttcctgcgc gaaaatggcg tagtgccgga aaaatgcgat cttaactcca tcctcttcct
     2101 gctgactccg gcagaagata tggccaaact tcagcaactt gttgccctgc tggtacgctt
     2161 cgaaaaactg cttgagtccg acgcgccatt agcagaagtg ctaccttcca tctacaaaca
     2221 gcatgaagag cgctacgccg gttataccct gcgtcagttg tgtcaggaaa tgcatgattt
     2281 gtatgcccgc cacaacgtga aacaactgca aaaagagatg ttccgtaagg agcacttccc
     2341 acgcgtcagc atgaatccgc aagaagccaa ctacgcctat ttacgcggtg aagtggaact
     2401 ggttcgtctg ccggatgcag aaggccgtat cgctgccgaa ggtgcgcttc cttatcctcc
     2461 gggtgtgctg tgtgttgttc cgggtgaaat ctggggtggt gctgttctgc gttacttcag
     2521 cgctctggaa gaagggatca acctgctgcc aggttttgca ccggagctgc agggtgtcta
     2581 tatcgaagaa catgatggtc gtaagcaagt ttggtgctat gtcatcaagc ctcgtgatgc
     2641 gcaaagcacc ctgttgaaag gggaaaaatt atgagtcagg ctaaatcgaa caaaatgggc
     2701 gtcgttcagt taaccatact gacgatggtc aacatgatgg gctccggtat catcatgctg
     2761 ccgacaaagc ttgccgaagt cgggacaatc tcaattatct cctggctggt gacagccgta
     2821 ggctcaatgg cactggcatg ggcattcgca aaatgcggta tgttcagccg taaatcaggc
     2881 ggtatgggcg gttatgccga gtatgctttc ggtaaatccg gtaactttat ggcgaactat
     2941 acctacggcg tctcactgct gattgctaac gtcgcgattg ctatttcggc ggttggttac
     3001 ggcaccgaac tgctcggcgc gagtttgtcg ccagtgcaga ttggtcttgc gaccatcggg
     3061 gtgctgtgga tttgtaccgt ggctaacttt ggtggtgcgc gcattaccgg gcaaatcagt
     3121 agcattaccg tgtggggggt cattattccg gtcgtcggtc tgtgcattat tggctggttc
     3181 tggtttagcc cgacgctgta cgttgattcc tggaatccgc atcatgcacc gttcttcagt
     3241 gcggtaggtt cttccatcgc tatgacgctg tgggcttttc ttggtctgga gtctgcgtgt
     3301 gcgaatactg atgtagtgga aaacccggaa cgtaatgtgc caatcgcggt actcggcggt
     3361 acgttaggtg cggcggtgat ttatatcgtc tccaccaacg tgattgccgg gattgtgcca
     3421 aatatggagc tggcaaattc aacggcacca tttggtctgg ccttcgcgca gatgttcacg
     3481 ccggaagtgg gtaaagtcat tatggcgctg atggtgatgt cctgctgcgg ttcgctactt
     3541 ggctggcagt tcaccattgc ccaggtgttt aaatcttcat ctgatgaagg ctacttccct
     3601 aaaattttct cccgtgtaac caaagtggat gcaccggtgc agggaatgtt gaccattgtg
     3661 attattcaga gtggattggc actgatgacc attagcccgt cgctaaacag tcagttcaac
     3721 gtgctggtta acctggccgt ggtgaccaat atcattccgt atattctgtc aatggcggca
     3781 ttagtcatta ttcagaaggt cgctaatgtg cccccctcaa aagcgaaagt tgcaaacttt
     3841 gttgcttttg ttggcgcgat gtatagcttt tatgcgctgt actcatccgg ggaagaagcc
     3901 atgctgtacg gttccatcgt gaccttcctt ggttggacac tgtatggtct ggtatcacca
     3961 cgctttgaac tgaaaaataa acacggttaa taaaacaaag taaaaaaggg cgattgaatg
     4021 atcgcccttt ctgtttgttg gcaaatagtt cgttatttat gccgcatccg gcagatgtag
     4081 ggtctaaacg cgatcgaaca cagcaactat ttgcgtaatt atatggggca tctgcgacaa
     4141 aaactggaac aggatccggc ccggcctcgc cattttatta ctgaaaccgg tattgggtat
     4201 cggtttatgc tttgaaaaaa gtggcaatgt atggataaag tcatttttta ttcaaatata
     4261 aggaacttaa tatttaaaaa atgttccata caattcctca tcataaatga caccgtagca
     4321 cacaaggagc aagtagcgat gcgtgaaatg aatcagttag taaatactga agattcagcc
     4381 tggccaataa tccagaactg gttaaaagac gccacaaacc ataccgaact cttaccggtt
     4441 aataaagatt tagccgaaac cgcactttat caattacagg tgacaacaaa atcaccgatg
     4501 ggtgcgcttg tctatggctc cggaggtctg ctgattgata atggttggtt acgcatagca
     4561 ggatctggtc acccccggtt gccacgagat ccagtaagtt ggacacaacg ccctgaattc
     4621 gcaggcgtgc gggctttacc catcgctgac gatgtcgcag ggggaatttt tgctcttaat
     4681 ggtggcgatc ttggggaaga tactggctgc gtttattatt ttgcgccaga tacgctgaat
     4741 tgggaaagtc tggaagttgg atattctgag tttcttcagt gggcattatc cggtgattta
     4801 gataccttct atgaaaatgt acgatggcag caatggcgag aagacgtaat aaaattatcg
     4861 gcaacggaag ccttcacgtt ctaccctttc ttatgggtac aaagtgaaga agcaagaaca
     4921 agaaaagtta tttcgcttac tgaactctgg gaaatgcagt atcaaatgaa agaaacattc
     4981 acgcaataag acatgttcag gtccgggttt cattcccgga ctcactattt taatgtgtat
     5041 ttatcgcttt caattcttgc tggcaatttt ctgactggca gcgtcgtaac tcaataatcc
     5101 cgacatgcat catcgagcca aaaaatccgc tttcgctgac ataatcctca ttgactatcg
     5161 gtcgagtgcc gggcttgccc tggcaaaccc gcataaaaaa tgaggtgtag cattgccaac
     5221 cgggatttaa accgaccttt tcttgtgctg acatattatg aagataagta agttcttttt
     5281 cgttcccgcc tgacaactgc cagcggcggg tattatcagg taagttatca ccacggcaat
     5341 ttgtcttaca ccgaaaaggt gcaatattta ccgcaatgct acccgttaat cctctggttt
     5401 tatctttagg ggtcagtaac c
//
LOCUS       DBBGLQ010000199         5386 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.199, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000199 DBBGLQ010000000
VERSION     DBBGLQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.199"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(76..1359)
                     /gene="aroA"
                     /locus_tag="ACIYSB_005152"
     CDS             complement(76..1359)
                     /gene="aroA"
                     /locus_tag="ACIYSB_005152"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGC5431994.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1430..2518)
                     /gene="serC"
                     /locus_tag="ACIYSB_005153"
     CDS             complement(1430..2518)
                     /gene="serC"
                     /locus_tag="ACIYSB_005153"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGC5431995.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2717..3409)
                     /locus_tag="ACIYSB_005154"
     CDS             complement(2717..3409)
                     /locus_tag="ACIYSB_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGC5431996.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3539..5299
                     /gene="ycaO"
                     /locus_tag="ACIYSB_005155"
     CDS             3539..5299
                     /gene="ycaO"
                     /locus_tag="ACIYSB_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGC5431997.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1376 a         1395 c         1278 g         1337 t
ORIGIN      
        1 gtccgaaaaa aaacgccgac aatgtgccga cgttttgata aagaaagatt tggctattta
       61 ttgcccgttg ttcattcagg ctgcctggct aatccgcgcc agctgctcga aataatccgg
      121 aaatgttttg gccgtgcatt tgggatcaag aatcgtcact ggtgtatctg acaacgccac
      181 cagcgagaaa cacatcgcca tccggtgatc attgtatgtc gcgatctcgg caaagttcag
      241 tttttccggt ggagtgatac gaatgtaatc gtgcccctct tctacttccg caccgacttt
      301 acgcagttct gttgccatcg caaacaggcg atcggtttct ttaacacgcc agttatagat
      361 attgcgcagc gtggtggtgc cttttgcaaa taacgccgcc gtggcaatgg tcatcgccgc
      421 atcgggaata tggttcatat ccatatcaat agcgttcagt tcaccacgcg tgcaggaaat
      481 ataatcatcg ccccagcaaa tggtcgcgcc cattttttcc agcacatcag caaagcgaat
      541 atcaccctgc atactgttac gtccaatacc ggtcactttt acagtgccgc ctttgattgc
      601 tgctgctgcc agaaagtaag aagccgaaga tgcatcgcct tcgaccaaat aagtacccgg
      661 agactgataa gactgcccgc cttttacgac aaattgttga tagtgctgat tttcaatttc
      721 aacaccaaac gtcttcatca gattgagtgt gatgtcgata taaggtttag aaaccagatc
      781 gcctttaata cgaatcaccg tatcttccgg cgcaagaggc gcagtcatta acagtgcggt
      841 gaggaattgg ctggaaacgg agccatcaac gtcaacgttg ccgccggtaa agccgccctg
      901 taaacgcaac ggcggataat tttcttgttc caggtaagtg atcttcgccc cgcccaggcg
      961 cagagcatcc accagatgac caatcgggcg ttctttcata cgcggctcac cggtcagcac
     1021 aatatcattg ctacccagac aaagagctgc cgccagcgga cgcattgccg ttccggcgtt
     1081 accgaggaac aactccaggg caccttctgc gtgtaatgga ccgccgttac cgataatttc
     1141 gcaacgcgta cgatcggctg aaagcgtata gcttacccct aacgctgtta atgcattcag
     1201 catatggcgc acgtcatcgc tatccagcag attggttaat actgttttgc cgtgtgctaa
     1261 tgccgccagc aataaagcgc ggttagaaac gctcttggaa ccgggcagat taatagtgcc
     1321 atcgacacga gcgatgggtt gtaacgtcag ggattccatg aactcaactc tctacaacag
     1381 aaataaaaac cccacaggct ggctgtgggg attaagcaaa atttcggcat tagccgtgac
     1441 ggcgttcgaa ctcaaccatg aagtctgtca gcgctttaac gccttccagc ggcatggcgt
     1501 tataaataga cgcgcgcatt ccgccgacca cacggtgacc tttcagtgca tgaaggccag
     1561 cagcaaaaga ctcttcaaga aacaatttgt caagcgcact gtccgccaac tggaacggca
     1621 cgttcatccg cgaacggtta gctttcgcca cgtcattgcg gtagaaatcg ctgttatcaa
     1681 tcaccccata tagcagttct gctttttgct gattgatttt atccatttca gctacaccgc
     1741 cgttcgcttt cagccattta aagaccagac cagatagata ccaggcaaat gtcggcggcg
     1801 tgttaaacat ggagccgtta tcgttgagga tggaataatc cagaatcgac ggacacgcga
     1861 tattcgcttt gcccagcaaa tcttcacgaa cgatgacgat tgtcaggcca gccgggccga
     1921 tatttttctg cgcgccagcg taaattacac cataacggct gacgtcaatc ggacgggaaa
     1981 gaatggttga agagaagtcg gcggcgacca ccacatcttt gccgaagtct ggcgtctcgt
     2041 cgatggcgat accgtcgatg gtttcattcg ggcagtaatg catataagcc gcattatcag
     2101 agagttgcca ttcacgcatt ggcttaaccg cgcgcagacc atcaacagtc actttggcgt
     2161 caaagacatt aggcgtgcag tattttttcg cttctttaat ggcacttgcc gcccagtaac
     2221 cggcatcaac ataatctgcg gtggttttat caccgagaat attcagcggt accgcagcaa
     2281 actgaccgcg accaccgcca tggcagaata ataccttgta gttggagggg acattaagaa
     2341 gatcgcgaaa atccttctcg gcttcctctg caacctgaat gaactctttg ccacggtgac
     2401 tcacttccat caccgacgta ccaagaccgt tccagtcgcg cagttcctgt tgagcctgtt
     2461 taagcacctc tgccggtagc attgccggac cagaactaaa attgaagatt tgagccattt
     2521 cccctcacca cgttgcgttg ctattaaccc gcggtcaatc gattgcgacc acaggtaata
     2581 cattattcct gtggctatcg gttttatcat tcagtgacac acatcgcaat gagtaaaacc
     2641 gttgagggca aaaatgtggc ttgcatcaca caaaagaatc tctcggatta ttagcaaaaa
     2701 gccgatgttt ttactgtcag gtcacccgtt ttgcccgact tgccttcgtc cattctggat
     2761 ttgcacagcg cggacataat tgtttttccc ccgctttcat atgaatgatt tcactacaac
     2821 gaatacaggc atagtccgcc gactccggca ccactttgta acgctgcgta caatcactgg
     2881 gtaaaataag taaacccggt ttcaccttgt catcttcaaa gaaatagaca ctgatttgcc
     2941 cgttggtttc gagaatcgcc agacgtacct gccccagctg ctccacgcca cgcaatcgta
     3001 gctccataaa gaactcaaat tccgtcatgt tggagttatt gagtttcgac caggccagct
     3061 cgccatcttc aataatgaca actggcttgc cttccagaag atcttccagt ttttcactgt
     3121 gcgccatcaa ccacattacc aggcggtata acaacgccag agtaataaag acgataagta
     3181 ccgggaccat cggcacatca tcataaaacg ccacatctcc cgccgctgat cccagcgtca
     3241 gaatgattaa aacttcaaac agcgacatct gccgcacacc gcgtcttccg gtcattttga
     3301 ggaacaaaaa gaccagtaca aaggtataaa gactacgtag tgcaacttcg ccaaggaaat
     3361 caaaaggcac tttatcaaat gccatacggt ggagatcgaa cgccttcatt tttatgctct
     3421 taacgtggtt tatccgctaa aagcatagtc aacagttcat cggcatggcg gcggtgatag
     3481 cgcctttgag ataaaacccg tattattgcg cgctttccgt acgactaaag tgattttcat
     3541 gacgcaaaca tttatccccg gcaaagatgc cgctctggaa gattccatcg ctcgcttcca
     3601 gcaaaaactt tcagacctcg gctttcagat tgaagaggcc tcctggctga atcccgtgcc
     3661 taacgtctgg tctgtacata ttcgcgacaa agagtgcgca ctgtgtttta ccaacggtaa
     3721 aggcgcaacc aagaaagcgg cgctggcttc tgcactcggt gaatatttcg agcgtctctc
     3781 aaccaactac ttttttgcgg acttctggct gggcgaaacc atcgccaacg gtccgttcgt
     3841 gcattatccc aacgaaaaat ggttcccact gaccgaaaat gacgatgtgc cagaagggct
     3901 gctcgatgac cgtctgcgcg cgttttatga tccggagaat gaactgaccg gcagcatgct
     3961 gattgaccta caatccggta acgaagatcg tggtatttgc ggcctgccgt ttacgcgtca
     4021 gtccgacaat cagaccgttt atattccgat gaatatcatt ggtaacctgt acgtctccaa
     4081 cggtatgtcc gcaggtaata cccgcaacga agcacgcgtt cagggattgt ctgaagtttt
     4141 cgaacgctac gtgaaaaacc gcattattgc tgaaagcatc agcctgccag agatcccggc
     4201 agacgtgctg gcgcgttacc cagcagtagt tgaagccatc gaaacgctgg aagcggaggg
     4261 tttcccaatc ttcgcttatg acggttcgct tggcggccag tatccggtga tttgcgtggt
     4321 actgttcaat cctgctaacg gtacctgctt tgcctctttc ggtgcgcatc ctgattttgg
     4381 cgtagcactg gaacgtaccg tgaccgagct gctgcaaggt cgtggcctga aagatttgga
     4441 tgtgtttact ccgccaacct tcgatgatga agaagtcgct gaacatacca acctcgaaac
     4501 gcactttatc gattccagcg gtttaatctc ctgggacctg ttcaagcagg atgccgatta
     4561 tccgtttgtg gactggaatt tctccggcac cacggaagaa gagttcgcca cactgatggc
     4621 tatcttcaac aaagaagata aagaagttta tattgccgat tacgagcatc tgggcgttta
     4681 tgcttgccgt attatcgtgc ctggcatgtc cgatatttat ccggctgaag atctgtggct
     4741 cgcgaataac agtatgggca gccatttacg tgaaacgatt ctttcgctac caggcagcga
     4801 gtgggaaaaa gaagattacc tgaacctcat cgagcaactg gatgaagaag gttttgatga
     4861 ctttacccgc gtgcgtgagc tgttgggtct ggcgaccggg tcggataacg gttggtacac
     4921 cctgcgtatc ggtgaattaa aagccatgct ggcgctggct ggtggcgatc tggaacaggc
     4981 tctggtctgg accgaatgga cgatggagtt taactcatca gtatttagtc cggaacgcgc
     5041 caactattat cgctgcctgc aaacgttgtt attactggca caggaagaag atcgccagcc
     5101 gctgcaatat ctgaatgcgt ttgttcgcat gtacggcgca gatgccgtag aagccgccag
     5161 tgcggcaatg agcggcgaag cggcgtttta cggcctgcaa ccggtagata gcgatctgca
     5221 cgcgtttgct gcacatcagt cgttgttgaa ggcctacgaa aagctgcagc gcgccaaagc
     5281 agcattctgg gcaaaataaa atcaaatagc ctacgcaatg taggcttaat gattagtctg
     5341 agttatatta cggggcgttt ttttaatgcc ccgctttaca tatatt
//
LOCUS       DBBGLQ010000200         5346 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.200, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000200 DBBGLQ010000000
VERSION     DBBGLQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.200"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..524)
                     /locus_tag="ACIYSB_005156"
     CDS             complement(<1..524)
                     /locus_tag="ACIYSB_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HGC5431998.1"
                     /translation="MKKVFAVSVVGAACVFAANAGAEDGKSGFYLTGKAGASVVSLSD
                     QRFLSGDEEETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NMDKGKGSWSDGYWRDDLKNEVSLNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKT
                     TGISTWDCGDGYSGR"
     gene            complement(742..1110)
                     /locus_tag="ACIYSB_005157"
     CDS             complement(742..1110)
                     /locus_tag="ACIYSB_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5431999.1"
                     /translation="MSAQEKTVQIDSFDAKFLDINTLSKLIYERKSFVIENVSDVSET
                     VKRVEHEIEKRKLSCRVYTEYRSTALAGSLWSPTVILGVASAVAIGVHNLSTWNPDYE
                     IGKNYIKRRLSVKYKKAEVL"
     gene            1393..1571
                     /locus_tag="ACIYSB_005158"
                     /pseudo
     CDS             1393..1571
                     /locus_tag="ACIYSB_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1794..2072)
                     /locus_tag="ACIYSB_005159"
     CDS             complement(1794..2072)
                     /locus_tag="ACIYSB_005159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432000.1"
                     /translation="MANKLNCENLVFPDDFKLSVTFKSLTSREGNERLHNRYVLSNVA
                     GVCFMHGLDEGEGTDDVSILSKEGYNKRWEHYTTNNVFDLIEEREVIC"
     gene            complement(2126..2689)
                     /locus_tag="ACIYSB_005160"
     CDS             complement(2126..2689)
                     /locus_tag="ACIYSB_005160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432001.1"
                     /translation="MFYECAISPEALFEIAIDRRNYRDFIKGFSTGGNFLYSELPKLK
                     RNKKQLLGLLNANHSELQKKRLEDLIIFLKNNKVSRVYDYVGDMSWSDNISAVNRIEQ
                     FDHVVSSTPCDNLDVTNIDDFFGLNYARQKIVARIAEDMISIISRLLKTSEHIIIVDP
                     YFSDKQRWWNVFISLLSVSANNSYKKI"
     gene            complement(2689..4311)
                     /locus_tag="ACIYSB_005161"
     CDS             complement(2689..4311)
                     /locus_tag="ACIYSB_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGC5432002.1"
                     /translation="MKLTKLVLENFRSVRERQEIDFAPVTLLLGPNSAGKTTILIALF
                     YLQQILDREQCDPIYLEAMGNRRIDGFRSLVHNGDLNNVITIGIGFDPEQTIGVEYKT
                     YLEELGDIFDIHFIKMKDIADETEKVYVEFKIAWSQLHRAAYIKNYAVSINNEFIGEL
                     SNDEYTKSSLVNRLNFTHPLLLPIDYNLWLESIDKEGLDTIASEFEISLNDLIPGLER
                     NKSGATERVKTVDYIFEEDKEKPMFPVFFKTKIGALPQLGRTVDTNLKVFDISEPVEY
                     FNRDIITRVLTQVFTSPLDKLNNYLKASVSIGPLRIIPDSSFTPNPYPEQNGWFDGTS
                     AWDKLCLSQQNDSDLIKKVSDWFSNRDKLNTNYNIFSGGEFDIKTSPQLLGLKDNVYF
                     CKIDSSQMLFPNQVGVGLTQIAPLIIAANIVQDGLIAIEQPELHIHPALQLAVGDLFT
                     QYPLDVKRPMFLVETHSEHILLRILKRIRQTTDNELPESNYPVKPDFISVIVFEDNNG
                     STVTRKIDITDDGDFKQKWPKGFFEERRGELF"
     gene            complement(4320..5346)
                     /locus_tag="ACIYSB_005162"
                     /pseudo
     CDS             complement(4320..5346)
                     /locus_tag="ACIYSB_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680721.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         1679 a         1062 c          849 g         1756 t
ORIGIN      
        1 cgaccactgt atccgtcccc gcaatcccag gtgctgatac cggttgtttt ctgatgaatc
       61 ctggcgtaac caatccctgc tgatacccat ggtgtgaatg cactgtcatt ccggaagtca
      121 tagtatgcat tcagcatcag agtgttgagt gatacctcat tcttaaggtc atcacgccag
      181 taaccatctg accagctgcc tttaccttta tccatgttat acttcgaatc agcttttcca
      241 cgtgcgtaaa actccagttc tgtacgaacc ggaatactga actgcggata aaaatcataa
      301 ccggctgcga taccgccact gaataccgtg tcatggtcat ccccaccttt atactttgac
      361 gtttcttctt catctcctga caggaaacgc tggtctgaaa gtgacaccac agaggcaccg
      421 gctttaccgg tcagataaaa accgctttta ccgtcctcag cacctgcatt tgctgcaaat
      481 acacaggctg cgcccaccac tgaaacagca aaaacctttt tcatttcaat tatctccgtt
      541 attgtatcat cactttaata tatattttta atatttaaaa accggactaa aggcagcctc
      601 ccccctccac actacggaag ccagactttt actctacaca aaataaatat atttcctcag
      661 tgaggacgtg aacgccagtc tgaaattatg tgtatacaac ctcaccagaa tacgaacagg
      721 caggggcact ggagtaaccg atcacaatac ttctgccttt ttatatttta ccgataatct
      781 ccgtttaata tagttcttac cgatttcata atcggggttc catgtagata aattatgaac
      841 acctattgct actgcactcg ccactcccag aattacggtt ggtgaccata aagaaccagc
      901 taaagctgtg ctcctgtact cagtatatac acgacaactt aatttccttt tctctatttc
      961 atgctccact ctttttaccg tttcactaac atcggaaaca ttttctatca caaaactctt
     1021 acgttcataa ataagcttgc ttaatgtatt tatatctaaa aatttcgcat caaaagaatc
     1081 aatctgtact gttttttctt gtgctgacat cttcttttcc tggctggtat ggcaagtgac
     1141 ggagacaaga gaaacttttt aagctcagtt atctccgcca tcattttcca cgaatgacaa
     1201 gtaattttgc ctattttaaa accatgcaaa aggcagggta aaaagagaaa attcgatcga
     1261 atcgatcgac aaaatcgatc atgcatgatg aagatttctt atcgaagaca tgaaaatggt
     1321 aaggaatggt ttaagaggta attgcaacgg catggaagta ttgagaccag aacagcacga
     1381 gataaagcgg ctgaacgtca gatgagccac atggacgtaa ctcagtgcag gcagcgtata
     1441 tccataaagc ggagcatctg gatgagcgca agctgatgct gcagtggtgg gcagattttc
     1501 tggatgccaa tcgggagaga gcgattacgc cgtttgacta tgcgaagatc aatcggggta
     1561 atggcgagtg agttgtttat caaatttgct aaattaaaac tatttaacct gccttatagg
     1621 gacgattggt tggctatttc actagaagta atgtagataa cagaagcctg gtctatcatt
     1681 ttgatagacc aagtcaagat taatagaatt agatttttta ttggcgtgtt gaataaattt
     1741 taaagggtcc taataagttt gtaggattta attgaaaaca gtaagggaaa tacttaacat
     1801 ataacttcac gctcttctat taaatcaaaa acattattcg tagtgtaatg ttcccatctt
     1861 ttattatatc cctctttcga caaaattgag acatcatccg ttccttctcc ctcatctaaa
     1921 ccatgcatga aacaaactcc agcaacatta gaaagaacat atctattatg cagacgttca
     1981 ttaccctcgc gagaagttaa agacttaaat gttactgata atttaaaatc gtctggaaag
     2041 acaaggtttt cgcagttcaa cttattagcc aaataattca ccgttgggga gttttcttta
     2101 ctcccatcaa atataacatc tatctttaaa tttttttata agagttatta gcactaacgc
     2161 taagtaatga aataaataca ttccaccatc tttgtttgtc actaaagtat gggtcaacta
     2221 taataatatg ctctgatgtt tttaataagc gtgagattat agatatcata tcctcagcaa
     2281 ttctagcaac aattttttgc cgagcataat ttaacccaaa gaaatcatct atattagtta
     2341 catcaagatt atcacaagga gtagaactca ctacatgatc aaattgttca attcgattta
     2401 cagcagagat gttatctgac catgacatgt caccaacata atcataaacc ctactaacct
     2461 tattattttt caaaaaaata ataaggtcct ctaatcgttt tttttgaagt tcactatgat
     2521 tagcatttaa tagcccaagc aattgttttt tatttctctt cagctttggt aattcactat
     2581 agagaaagtt accgcccgta gaaaatcctt ttatgaagtc acgataattt ctccgatcaa
     2641 ttgcgatctc aaacaaagcc tctggtgaaa tggcgcattc gtagaacatt aaaaaagctc
     2701 tccccttctt tcttcgaaga aacctttggg ccatttttgt ttgaagtcac catcatcagt
     2761 aatatctatt ttcctagtaa cagtcgaacc attattatcc tcgaaaacga tcactgatat
     2821 aaaatcaggt tttactgggt aatttgattc aggtaattca ttgtccgttg tttgtctaat
     2881 tctttttaaa attcgaagca aaatgtgctc actatgtgtt tcgactaaaa acataggtct
     2941 tttaacatca aggggatatt gggtaaacag atcacctaca gcgagttgta aagcgggatg
     3001 aatatgtagc tcaggctgct ctatagcaat taatccatcc tgaactatat tggctgcaat
     3061 aataagaggt gctatctgag ttaatccgac accgacttga tttggaaata acatttggct
     3121 tgaatctatt ttacagaagt aaacattatc tttcagacct aaaagttgag gggaggtttt
     3181 tatatcaaat tctcctccac taaatatatt ataattagtg tttagtttat ctctattgga
     3241 aaaccaatca cttacttttt taatcaaatc actatcattt tgttgagata aacacagctt
     3301 atcccatgct gatgtaccat caaaccatcc attttgttct ggataaggat ttggcgtaaa
     3361 tgaagaatca ggtataattc ttaacggtcc aatagatacg ctagccttta aataattatt
     3421 taatttatct aacggagaag taaaaacttg cgttagcact ctggtgatta tatctcgatt
     3481 aaaatactca actggctcgg atatatcaaa aactttcaaa tttgtatcaa cagttcttcc
     3541 cagttgaggt aacgcaccaa ttttcgtctt aaaaaaaaca ggaaacattg gtttttcttt
     3601 atcttcttca aatatataat caacagtttt tactcgctct gttgcaccac ttttattacg
     3661 ttctaagcca ggaattaagt catttaaaga tatttcaaat tcactggcaa tcgtatctaa
     3721 tccttcctta tcaatagatt caagccataa attataatca ataggaagaa gcaacggatg
     3781 agtaaagttc aatctattta ctaaagatga tttagtatac tcatcattac ttagctcccc
     3841 aataaattca ttatttatag aaacagcata gttctttata tatgctgctc tgtgcaattg
     3901 tgaccatgcg attttaaact ctacataaac cttttcagtt tcatctgcaa tgtctttcat
     3961 ttttataaaa tgaatatcga aaatatcacc aagttcttct aaatatgttt tatattctac
     4021 acctattgtt tgttcaggat cgaaaccgat accaatagta attacattat tcagatcccc
     4081 attatggact agagatctaa atccatctat gcgcctgttc cccatagctt ctaaatatat
     4141 aggatcacac tgctcacggt ctaatatttg ttgaagataa aataatgcaa ttaaaatagt
     4201 agtcttgccc gcgctgtttg gccccaatag taatgttaca ggtgcaaaat caatttcctg
     4261 ccgctctcta acagatctga aattttcaag tacaagttta gttaatttca taatgaacct
     4321 tacatgaaat atttattcga taggtttgat gctaaaagtt gaagttgcat tgcgctttta
     4381 tcataaatgc ttagtccttt tttaattttt tataaataat ctcatactca tcttgtagat
     4441 gcttaggtgg gattaatatc tctatattct gcaattcctg tgcattaata ttagccatgc
     4501 caacaatact tttacacata ttcatcaatg tgattttgcc atggatggaa ttaagatacc
     4561 ctgaaatata gtagttattt ccgatagaat taggtctaac gcggattaaa tatccagcaa
     4621 atgccatagg cctgtcttct tcatacacag ctgttttacc aacaagttcc ttactgttag
     4681 tacgattgaa aagaagatcg ccttctttaa ccaaatattt atccttttct tttacactga
     4741 gatcaagata ttttaaatct gtaaaatccc acctaccttg atatgttata ttacccatcc
     4801 gtaatatagg atattcacca gaatcaatag aggcttttgc tgaagttcca taatttactg
     4861 aatcaaccaa gtcgcgaatc gttcctttcg ggaaacgatg tatgttgttt gcaggagttc
     4921 caaacatctc tagaaactta gcccgcaaaa aatcatcagc cagtttgatg gcttgttcgc
     4981 gcttttggcg tatagcatca gctttatcaa gaatagtagc aatgcgtttt tgttctttta
     5041 atgaaggaag cctaatttta atatcttgta tcttttttac agacaaatgt tttacagtca
     5101 caacatctgt tgtggcatga attttatcca attccttttg caagaaatga tatagaaaat
     5161 ttttatcaag tgtttcagga tttggcgtaa ttttacatac ccgttgattt aacaacgcat
     5221 cagtaccttt ccaatactca cggttgaaat ctccatccat cccaactagt aaatcacctt
     5281 ttttaataag atattttaag tcataactgc cttcataata agtttcggtt tttcctgaaa
     5341 gaatat
//
LOCUS       DBBGLQ010000201         5313 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.201, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000201 DBBGLQ010000000
VERSION     DBBGLQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.201"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(109..675)
                     /locus_tag="ACIYSB_005163"
     CDS             complement(109..675)
                     /locus_tag="ACIYSB_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGC5432003.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWIDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            complement(2240..3844)
                     /locus_tag="ACIYSB_005164"
     CDS             complement(2240..3844)
                     /locus_tag="ACIYSB_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGC5432004.1"
                     /translation="MNPDYVQSLQTSHHFPADLTYRLFPSELAYLIDDLYESTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMNPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKVVNRGYPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALVEGLNEHPEAGVISDEAVTFFRSYLKNYPGLLNKAWSGQ
                     PFDFGRADEKYHIAPRLTFSLMSQPDVFTNYINKNDVLAWGSGFLSRFLFSQVVSSSW
                     IRDYTRGEFRTISTLENFHKKINGFLLNHNINFPGMSTERKTLKLAKKALGEWQENQI
                     KIERKALAGGEWEHIRDIVLKAGSNILRIAGIFTCYCYKDAEEIESITLFKAMHLMDW
                     YLEEASTIFYPMSARCQFEQDACELYAWIMTRIRQNNWRAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLICQGYLCIIRMRSHQALYVSVYCNNGYLLPLGAMYYEQFDIVLPENIHK
                     AKAYHVNIPPELIPSFASPFSEYSLF"
     gene            4278..4484
                     /locus_tag="ACIYSB_005165"
     CDS             4278..4484
                     /locus_tag="ACIYSB_005165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432005.1"
                     /translation="MSLPNPLCVDTDVSKTTLDITASNTIERFSVASGADGVDFIIAE
                     LRQHDMAFVLMEGRHNLCVSARRF"
     gene            complement(4496..4897)
                     /locus_tag="ACIYSB_005166"
     CDS             complement(4496..4897)
                     /locus_tag="ACIYSB_005166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432006.1"
                     /translation="MVSSLRIQATSTAVVITPMPLMLLRCSAGSLKWFRMWVFIFQRF
                     DDINVFFALRVSRVQPVLLSNWHCDQLVSANDYGIKNLTFCIRQSPDKAVSFRMVINN
                     CSHLGKKTAHHAGQSSPDIPSHGQSHELAWG"
     gene            complement(5028..>5313)
                     /locus_tag="ACIYSB_005167"
     CDS             complement(5028..>5313)
                     /locus_tag="ACIYSB_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGC5432007.1"
                     /translation="FSRRAPFRKPTSSDARPSSDDTPLSGGCSDTRKASDKKSGGSAV
                     LWVKRGDVEAMQKAMERACYLVKYETKQHDGSGQRNYGCSRGPGRLLDGR"
BASE COUNT         1622 a         1167 c         1102 g         1422 t
ORIGIN      
        1 caggttcagg gtgaatgatc tccgttgact tcagttcaca tcattaatca aggcggtaag
       61 gttttaccga cggagtgttt ccaggagcgg aggctcaggc cttaagcttc agaagtgagg
      121 cttacgagga cgccctgcag caggacgttc aggaacttca ttgcagccgt aggcacacag
      181 cccgtctttt tgttcttctt tcgccagata atgcagggca ccgcgaatat tatcgatgct
      241 ttcaggatcg ttgtggcgca caggaatgtt gatgttgtat gtataatgcg gctgatatgt
      301 acagcggtgt gccgaaccac tgttatgcgt aatagaccgc cagcattccg taatccgctc
      361 cgcaaaggga tatatttttt taaccctctg acgatcgatc cagaaaaccg catgcgcatg
      421 aaaaccatga tcagccgtcc attcaatcac ccagaagaag ccaacgatat tttcaaattg
      481 ttccacctgc tccagcagca tacgcaactg caattcaagc cagcgatgat caggttgtaa
      541 gaaatcaggc gtatctttcc tgtagaagaa atccatgcgt aaggcccgca ggcaggaata
      601 acgtgccatc atttgattga tgtgattgtc aagtaaagcg gtaaaaaacc agtttggtgt
      661 gtaagattta ggcatgctga tgactcctga aagttaatga cgttttaagt gctgtaaacg
      721 aattaatgct ttcgctcata atcagatcta aaacggtaat aacttacaga cggtatttat
      781 caggcatgct gtaacagaat ggatacgtcg gttattaacg acacgtagcg ttgtgcagaa
      841 ataaatggat tatctgatgg tattagctaa agagatgaga taaacgagga gcgaataaaa
      901 tacagaatca ataaacatca caaaatactg caaaacacgc gataaaaacc cacgaccaga
      961 agttcaagaa acatattacg accaataaca tcataaacta caaacttgct ctcagtggtt
     1021 ctaaaaaaaa cgtatacaag gccaacacaa taaacttata cgccctcatc caatctgttc
     1081 ttaaggacat gaacgttaac cgcacacatt accagagtaa aaaataccac tggcatgatt
     1141 tttaccctgc atcttaaaca atacaacccg aaaaaaacat aatcaggaat tcactctgtt
     1201 aatttcattg tttatcctgc actgttaaac atgacgaaat catggacacc ttatcaaaga
     1261 gtaaacattc agttaatcag tgatgacaga agtattgata tttaccggaa tggttaaaac
     1321 atatcagata aacaatcatt gttaagcaaa tacagctcat gatagataaa attaaaataa
     1381 attattattt tactgaaaac aggaacagat ggatatcagc aatagcgtag tagagaataa
     1441 cttatctgag tatcatatga gttacagtag ttatgattga ggaatggaga aggtggatac
     1501 catgataatg gtgacaatgc agattagaca aggaagggag tgaatataac tggatgagaa
     1561 taagagtaat ggaatgtgta aagacagaga gggaaaatga agggcagaga tggatgataa
     1621 ataaaggggt agatactaat tatagtaata aacctaaatt aaaattatcc atgtttgatg
     1681 agtaaaaata agaaatcatc cgttctgaat acgaacatga caaaccatta tcttacagaa
     1741 aaaaatatca aatcattttt cttcactctc ctattgaaat gcaaacttga acagcatctc
     1801 ttttcagtaa cagtgataac cattcgttat tcaggcagag accaacatta tcatatacac
     1861 ctgcactcat cacgacaaaa atcaaacgaa gttatatatg aaaactcaca caatatggtt
     1921 aatgattagc tttaatctat gttaaaaata atctttcgat tcactttcga tcaataaaaa
     1981 aacgaaagcc atgcttccac cagtgcgcac actcctgtat tcaaccagct ctgacagcag
     2041 gtcattatga aaacaaaatt aaatcacaga tgtggtgcat gtgctgctac tgttgctacc
     2101 gtagcggttt tttcagagaa attctcattc accccccata attggtgaac atttgcatag
     2161 gtgcaggata tgcaaaacgc cttatcacca acctccgaat gaaggttggt tgattttgtt
     2221 ttatctgtgt aaggttattt caaaataagc tatactctga aaaaggtgac gcaaatgacg
     2281 gaattaattc cggtggaata ttaacgtgat atgctttcgc tttatgaata ttctcgggta
     2341 gaacaatatc aaactgttcg taatacattg cgcctaaagg aaggaggtaa ccattgttac
     2401 aataaacaga aacataaagt gcctgatgcg atcgcattct gatgatacat aaatacccct
     2461 gacagattaa ctgattgagt acaggtgtaa gtttttctgc tcttctcagt cgattgggac
     2521 catatctttc aatgtctgtt ttcctgatag cacgccaatt attctgcctt attcgggtca
     2581 taatccatgc atacagttca caggcatcct gttcaaactg gcatcgtgca gacatgggat
     2641 aaaatattgt gctcgcctcc tccagatacc agtccatgag atgcatagct ttaaaaagcg
     2701 taattgattc aatttcctca gcatctttat agcaatagca ggtgaatatt ccagctatcc
     2761 tcagtatatt agagcctgct ttcagaacaa tatctctgat gtgttcccac tccccgcctg
     2821 caagcgcttt tctttcaatc ttaatctggt tttcctgcca ctcccccaac gctttcttcg
     2881 caagttttaa tgtcttcctt tcggtgctca tgccggggaa attaatgttg tggtttaaca
     2941 ggaatccgtt aatcttttta tgaaagttct ccagggttga tattgttctg aactcacctc
     3001 tcgtataatc acgtatccag gaagaactta caacctgact gaacagaaac cgggaaagaa
     3061 atccgcttcc ccacgccagt acgtcatttt tatttatata attcgtaaag acatccggct
     3121 gagacattaa cgaaaatgtc agacgtggcg cgatatggta tttctcgtca gcccgtccaa
     3181 aatcaaacgg ttgcccactc catgctttat tcaacaggcc cggataattt ttcagatagc
     3241 ttctgaaaaa agtgaccgcc tcatcagaaa taacccctgc ctcaggatgt tcattgagcc
     3301 cttccacaag cgctttaagc gaaacatctt catagataaa attcggtcga accggacgtg
     3361 tcggtttatt tctttcgtga ttacgcagcg cctcttcttc ctgttccccc ggataccccc
     3421 ggttaacaac ctttcttaaa ttggcagcaa gcgctttctg ccgggtattc cagatctgta
     3481 gttcagtctt ataatctttg tttctctctt cgtattgttg aatgagtcga tcggcaaatt
     3541 cgtaacaggg gttcatcacc agtctgttta tcgttgtttt tcccgcgcct ggctctgcga
     3601 ttgccaacag ataaagtgag cagggttccg gcatgttggt gtgaggatga acaacgtcaa
     3661 ccaatgactg acaggagagt gagagcgttg ccagtacagt attaaaaatg agctccaacg
     3721 gaagttgggt actttcatat aagtcgtcaa tgagatacgc aagctcacta ggaaataatc
     3781 tgtaggttaa atcagctggg aagtggtggg atgtctgcaa ggattgcaca taatcgggat
     3841 tcattgtaat ggacctcctt cgcatactgt aatcaacgca aaacttactg ttcagttcag
     3901 aaatataccg acatagtgag tatgttcgct gacgtcgata ataaaatgac ttgtatccat
     3961 tcaccagtcc cgacgcattt atgatgctga caaaagcaaa acgacatgga ggtgcctcag
     4021 aagtctggca ccacagcaac aaacgatggg ggcatgaaac acagggaaca ttgcgcccct
     4081 gagcgttgag gatcttccac cttgtatcct ggttcttcgt tatcatgagc agcgaacaga
     4141 tttggcaaag cgtatgcagc ctggtgcccc ttagggttta agcccatatg taagcaaatg
     4201 gcgcatacgc tgagccactc atctgaaacc cgaacagttg tccgtactcc ataacgcatt
     4261 agcaagaatg ggcaataatg agtctgccaa atccactttg tgttgatacc gatgtgtcca
     4321 aaacaacgct ggatattacc gccagcaaca ctattgaacg gttttctgtt gccagtggtg
     4381 ctgacggcgt tgatttcatc atcgctgaac tgaggcagca cgatatggcc tttgtgttga
     4441 tggaaggccg ccataacctg tgcgtttcag cccgaaggtt ttgagatcgc cgtagttaac
     4501 cccaggcaag ctcgtgactt tgcccgtgcg atgggatatc tggcgaagac tgacccgcat
     4561 gatgcgcggt ttttttgccc agatggctgc agttattaat taccatccgg aacgagaccg
     4621 ctttatccgg actttgccgg atgcagaacg tcaggttctt aatgccatag tcgtttgcag
     4681 acaccagttg atcgcaatgc cagttactga gcaaaacagg ttgtacccgg ctcacccgca
     4741 aagcaaaaaa aacattaata tcatcaaagc gctggaagat gaacacccac atccggaacc
     4801 attttaaaga accagctgag cacctgagca acattaaagg catcggcgta atgactacgg
     4861 cggtgctggt agcctgtatg cgcaaactgc tgaccatcct gaatgcgata ctcagaaaga
     4921 acaaagaaat gtaatgaatc ctgccatcag gttactccat aattttgttg gtcaagacag
     4981 ttaattacgt gacgtatgct caggcaccgg atgccgtttt acagggctca cctgccatcc
     5041 agtaaacgcc ccggcccacg actgcaacca taattacgtt gaccagaacc gtcatgctgc
     5101 ttcgtctcat acttcacgag ataacaggct ctctccatgg ctttctgcat cgcttccaca
     5161 tcaccacgct tgacccagag cacagcggat ccaccagatt ttttgtcaga agctttccgg
     5221 gtatcagaac atccacccga caaaggcgta tcatcggaag aagggcgagc atcagatgat
     5281 gtcggtttac ggaaaggcgc ccgcctggaa aaa
//
LOCUS       DBBGLQ010000202         5060 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.202, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000202 DBBGLQ010000000
VERSION     DBBGLQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.202"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(96..557)
                     /locus_tag="ACIYSB_005168"
                     /pseudo
     CDS             complement(96..557)
                     /locus_tag="ACIYSB_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(638..2146)
                     /gene="ltrA"
                     /locus_tag="ACIYSB_005169"
                     /pseudo
     CDS             complement(638..2146)
                     /gene="ltrA"
                     /locus_tag="ACIYSB_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(2819..3516)
                     /locus_tag="ACIYSB_005170"
                     /pseudo
     CDS             complement(2819..3516)
                     /locus_tag="ACIYSB_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3801..4639
                     /locus_tag="ACIYSB_005171"
                     /pseudo
     CDS             3801..4639
                     /locus_tag="ACIYSB_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            4773..4859
                     /locus_tag="ACIYSB_005172"
                     /pseudo
     CDS             4773..4859
                     /locus_tag="ACIYSB_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase"
     gene            4868..5060
                     /locus_tag="ACIYSB_005173"
                     /pseudo
     CDS             4868..5060
                     /locus_tag="ACIYSB_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1135 a         1387 c         1313 g         1225 t
ORIGIN      
        1 ctggaatata gaatcggtag ctaacgatga gaaacccaac aatcccatcg ggtgtgacgg
       61 tggagtgatc ctacccacgt aatgtggaca cggccctaag cgagattatg gttttcaaat
      121 tgttccgggc tgagaccgcc acaggcactg tgacgacgcc agcgattgta atcacactcg
      181 atataattaa acaccgttgc ccgcattatt tcccggctgc taaagcgttc cccgtggata
      241 cattccacct tcagcgaatg aaagaagctt tccacacagg cattgtcata acagtagcct
      301 ttcgcactca tactgccacg caggttgtgt cgcttcagca gcgcctgata atcccctgaa
      361 cagtattgac caccacggtc cgtatgaaca atgacgcttt ccgggcgtct tctccgccac
      421 aacgccattt gccgtgcatc acaggccagt tgtgctgtca ttcgcggtga catcgaccag
      481 ccaatgacgg cgcgtgacca caggtcgatg actactgtga gatacaacca gccctcatcg
      541 gtacgcagta gagtaagagg cagggcgtag tcggactatt tccctgcctc tcctccccga
      601 accggacatg cacctttcag cgcatccggc tctccattta aacgctggcg aacgccattg
      661 ccacttctgt aaagcgcgat gtatacgtct ttctggtttc cgtcctcaga tagggattac
      721 cttcgggtag ccgccagcgg aacagcttct tgccttgccc caccaaccgg tacagtattt
      781 cgccgctgag cttgccgtga ttggttttac caaataaaac ccacgttttg ctctgacccg
      841 gtttcggtga tttacaccac cacctcatca gggaagcgat acttgtacgg tatttgcggg
      901 ccagccagtg agccagcttc tagaacacga cacggtcgat ataactgaag actttggcct
      961 taaaatcaac gaactgatag aacatggccc agcctttcag ttttcggttg agttgttcag
     1021 ccatatcgac tttgctttca ctgtagttgc ctgataacag tgctgtcagc gatgcggcga
     1081 agtttctggc tttctccagc gggatcgttg agaccacttg catctcgcca taacgactgc
     1141 gtttgcgaat gatcctgtgc cccagaaaga taaagccgtc attaacatgg gtgattttag
     1201 tcttatccat gttcagcctg agtttcagac tgccttcgag cacaccccga cactcctccc
     1261 tgatggcttc cgcctgtgct ttggtgcctt tgacgatgag gacaaaatca tcggcatagc
     1321 ggcagtacgc caccgcgggt ttccactgcc agttttctct gaccgccgta cttcggctcc
     1381 gttggatact gttattccag taccaccgat cttttcttgc tttcccgctc aggtagcgct
     1441 catgcaggta ttgatcgaac tcattcagca tgatgttcga caatagcggc gatataacac
     1501 cgccctgtgg cacaccttca ctggccgccc gaaagagacc gacatcgata tgtcccgcct
     1561 tgatggtttt ccacagcaga gtcatgaaac gtgcgtcact gatcctgcgg cgtacagcct
     1621 tcatcagcag tcgatgatgt acggtgtcga agtaactgga caggtcgcct tcaatcaccc
     1681 agcgtccccg ggtttcaccg cagtcggtga gctgtaattt caccgtgcgg atcgcgtggt
     1741 ggacactgcg ctcaggccgg aagccatatg agagcgtatg aaaatcactc tcccatatcg
     1801 gctccatcgc catcagcatg gcccgctgaa caatacgatc ccgcaacgcg gggataccca
     1861 gtggtcgcag tttgccattg cttttaggga tgtaaacccg cctggcgggc aagggctggt
     1921 agtggcctga gagtaattca tccctgagga tttgcagctc aacagccagt ctggcctgta
     1981 gcattgtttt gttcacgcca tcaacaccgg gggtattcgc cccctttgat gaaagcgtga
     2041 tccgcgccgc ttcagccagc cattctggtt gtgttatcag acgcagcagc cgttgaatcc
     2101 gtagggacgg atcggtggct gcccatgtgg caagcttgcg ttgcatttcg ctgattatca
     2161 aaggtcttca cctcgttagg tcagttaatt tacgtcgcag acacattcaa actgcttccc
     2221 ttcgccatgt aatgggcttt ccccatcgcg gactactacg gaagctccgc cagccagcgc
     2281 gtcatcggag ccatgccccc ttaacatccg tcgctgacct tccccggttt acctgactgg
     2341 actcagacat actgaggagg ctgcccgtcg cactctttat ccttgcttgc cgcaagttgg
     2401 cagaagtcag caacgcgagc gtgatagacg ctgctgcccc ggagtttcgc atacatgtca
     2461 aaacaccttc gaccggcagt gcttacgtat cactgccagt tcctcctgca cggcctgtca
     2521 gatcacgtag gccgtggtga cgttttcaac ccacagaggc ggattaacgg gttcatattc
     2581 ttaagccttt cagtacttaa ccttgaggat catctcggct tagtgatctc gcctcaatcc
     2641 ccgttgtcag cgggttacat caccctgcgg gcatgccgca ggtcactgcc gctcaggttc
     2701 tccaccgtca cacccggtgg gattgttggg tttctcatcg tgagttaccg gttcaatatt
     2761 ccagacagac tcgtggttca tttgagcgtc tatgctcgcc ctgaactccg ggcacacagt
     2821 acgtgatgtc acccacccac ttctggttcg ggccgctggc gctgaagtcc tgctccagca
     2881 gattctccaa tacgggcagg ccatgtgcac ggtagctggc cgggctgaac ttccggctgg
     2941 ctttcgcccg cagcccctga cgacgcaggc tggcggcaat ggttttaata ttgaactccg
     3001 gcagttcgtc agcaaggcgg ggagcaccgt atcgctgctt tgcctcaaag aatgccttat
     3061 ggacagcggc atcgcaggtg agccgaaact gttggcgcag gctcatctgg tgacgacgcc
     3121 tgagccagac ataccagccg ctgcgggcaa cccgaagtac acgacacatc gctttgatgc
     3181 tgaactctgc ccgatgattt tcgatgaaga catacttcat ttcaggcgct tcgcgaagta
     3241 tgtcgcggcc ttttggagga tggccagttc ctcagcctgc tccgccagtt gtcgtttaag
     3301 gcggatattt tcagcggcca gttcgctttc gcgctctgac gaactcattt gttgctgctg
     3361 tttactgcgc caggcataaa gctgagattc atacaggctg agttcacggg ctgtggcggc
     3421 cacaccgatg cgttcagcga gtttcagggc ttcgttacga aattcaggcg tatgttgttt
     3481 acggggcttc ttgctgattg atactggttt tgtcatgagt caccctggtt aagagtttac
     3541 tcacttagtc ctgtgtccac tattggtggg taagatcagt atgcgcttac agtcaaaggt
     3601 gttctgacat gtatgcaaaa cactgggaca tcaacgctta tcacactcgc gttgccgacc
     3661 tctgccaatt tgcggcaatc aagacaaaga gtgcgacggg cagtctcctc cctcagacag
     3721 gtgtgtacga tgcggagaca tagtaaacac ttgttcggag acatggttga tacttacaac
     3781 taaggcatca gaacccgttt atggagccgc ttatgcccag ggaagcgaga gataccatgt
     3841 cactacgttc tgagtttgtt ctgcccgcct tcagcacggt gcataacctg atggcccgca
     3901 atggccttct gcccggtctg gttgcggcag ccccggcgac gggccgcttc gaacacgccg
     3961 aaccagctct ggcagatgga ttttaagggg cactttccct ttggcggtgg acgctgccat
     4021 ccgttaacgc tgctggatga tcattcccgt ttttccctgt gtctggctca ctacgccaat
     4081 gaacaacgga cgaccgtgca gcaacagctg gtcagcgtat ttgaacacta cggagtgccg
     4141 gacaggatgc acctggacgg cactggagtt atggctgatg cgtcagggta tcaaggtggg
     4201 gcactccagc ccgtaccatc cacagacgca gggcaagctg gaacgttttc accgcagtct
     4261 gaaggcggaa gttttgcagg gtaagtggtt tacagatgcg ggtgagttgc agcgcgcctt
     4321 cgatcactgg cgcacagtct ataatcttga acgcccgcat gaagctctgg atatggctgt
     4381 tcctgcctca cggtatcagc cgtcagcgcg acagttcagg agcaatgtag agccgccgga
     4441 gtatgacgag ggtgtggtgg tcaggaaggt ggatatcagg ggtaaactga gtctgagagg
     4501 tgtcagtctg agtgcgggca aggcattcag aaatgaacgc ctggggctga aggagacgca
     4561 ggaagacggt cagtatgaag tctggtggta cagtacaaaa gtgggggtga tcgacctgaa
     4621 aaataagtcg atcaccatgg caaagaatgt taaaaagtgt tcaccatgtc cccgaacacc
     4681 tgtctaccat gtctccggat cgtacacagg tgaacccggg agggtcagcg atggatatta
     4741 aggaaacatg gcgctgatga cgcgctagcg aaatgcaacg caatcttgcc acataggcag
     4801 ctaccggcac ctccagacgg atagaacggc tactgcgtct gataacacaa ccagaatggc
     4861 ggcagccctg cagacagaaa atgtggtaaa agcgttcaga cagactctga ggctgggaaa
     4921 aatgacgagt cccctgatac atcattctgt actgttcagc tttctatcaa tcatttcatg
     4981 aaaaaaacgg gatgacttgt tcaatcatgc taaatgtttg taaaaacaca gcaataaatt
     5041 atattaaatt cagccttaat
//
LOCUS       DBBGLQ010000203         4996 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.203, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000203 DBBGLQ010000000
VERSION     DBBGLQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.203"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(8..1972)
                     /locus_tag="ACIYSB_005174"
                     /pseudo
     CDS             complement(8..1972)
                     /locus_tag="ACIYSB_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            complement(2038..2271)
                     /locus_tag="ACIYSB_005175"
     CDS             complement(2038..2271)
                     /locus_tag="ACIYSB_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGC5432008.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(2329..2856)
                     /locus_tag="ACIYSB_005176"
     CDS             complement(2329..2856)
                     /locus_tag="ACIYSB_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGC5432009.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGDDYGFSDDIPF"
     gene            complement(2882..3088)
                     /locus_tag="ACIYSB_005177"
     CDS             complement(2882..3088)
                     /locus_tag="ACIYSB_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGC5432010.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKEQVPAKRQNRGSRNSKDDGG"
     gene            3158..3677
                     /locus_tag="ACIYSB_005178"
                     /pseudo
     CDS             3158..3677
                     /locus_tag="ACIYSB_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3656..3847)
                     /locus_tag="ACIYSB_005179"
     CDS             complement(3656..3847)
                     /locus_tag="ACIYSB_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432011.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(3844..4266)
                     /locus_tag="ACIYSB_005180"
     CDS             complement(3844..4266)
                     /locus_tag="ACIYSB_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGC5432012.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGENMS"
     gene            complement(4313..4738)
                     /locus_tag="ACIYSB_005181"
     CDS             complement(4313..4738)
                     /locus_tag="ACIYSB_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGC5432013.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
BASE COUNT         1022 a         1546 c         1334 g         1094 t
ORIGIN      
        1 ttgttcatca ggcggcatcg gcctggttgt cacggtattc cgtggtttcc gtctctgcct
       61 gtggacgtag tgcacacatc cagccaggca cccagcggtt gtctttcata tggaactctg
      121 catgttcagc cgcatcacct ttcttcatct tctctgcgtc ccgtgcggca ccggacagtc
      181 cggcatcatt cagggctgcg ataatctgcg gctttttcag gtgtccgaag aagtttgctt
      241 ttgtcggctg ccaccagtcg cgcatgtgaa agccgatggc gctctccagc gtgtccagcg
      301 ggctgcgtga cgtgtgacca cactcgcggg tctggactcc gttaagactg cacgcggtac
      361 agaagctgag cagggataac agcacctcct ggctgagtga caggaacgtc gtcatgtccc
      421 gtgaccatcc ctccggcagc agggcagtaa gacgggcttt ttctgccatc atcgacagga
      481 atgcggcccc ttccttccct gacggcgcat cgctggtcag cgaagaatgt ttacattcca
      541 ggctgatttg tgctggttta ctgtacgctc cgctgccaaa cacattcagg cagagcgtcc
      601 atgccagcag tgtcagggat ttgtccggtt gctgcatcag tgccgcctgg actgccagcg
      661 tgcgttcgga agacattttc gtcagcagtg gcagactgat ttcctccact gacgctttct
      721 cctgcacctg ttccgtgcgg tcagcgtcgt cagccgcgtc atcttcactg cgcaactgca
      781 caccacgctg gacacacaca ttaccgtaac gccaggacac cacgacaccg cttccggcac
      841 gcatctccgg cgtccacgct ctgaccttcg ccatgcagcg catcagcttc atttctgctt
      901 ccagcaggtc ggattcctca cactgatttt ccagcgcgtc gtaacgcgtc atcagttcgt
      961 tcaggcgttc ttcttccgcc tccgtcagca ccgcttccgg ctccggcaga tagtggtatg
     1021 ttccggcatc ctcactgcag aaaccgacag gctccatacg tccggcgcac cattcccagc
     1081 cttcagcttc ccgaaggtgt tcagcgacag cctggagttt ttccagcagg gcagcatcga
     1141 gcgcaacaca gtccacatag ccaccctcgt cgtcgctgaa caagtcagtg cgcagttcat
     1201 ctggcgagaa ggcatcagcc cccacgaagc ggaatttact gttccccgcc accgccactt
     1261 cactttccgt aatcagattg cggataaccc gcacatccgg ttttcagccc catcccgact
     1321 gacaggcggc ttcaaacacc tgtacctgac gcgcggtgtc gttctccagc gccagcgcct
     1381 gacagtgctc ggtggtgatg cggtcttctg ccagcgcatc gaggataacc ggagcaaggt
     1441 cagccagttt cagcattcgc tgaacgtggc ggggtgaata gcccagcaaa tcaccgattt
     1501 gtgcaggtgt tttgccttcc tgcgccattg cgcggaatcc ggcaatctgt tcggcagggt
     1561 gcatatcccg acgctgaccg ttctcggtca tcgatgcggc agtcgccagc tcctgcggaa
     1621 tgaccttcac gcgtacaggc cagtcagccg gaaggatgtt gcgctctgcc agcatgttga
     1681 gtgctgccag tcggcgaccg cctgcggcga caccgtaacg gtcacctggc agggcatgaa
     1741 caaccagatt ctgtaacagg ccgacacctt taatggagtc cgccagttcg ctgacggact
     1801 ccgcagaata cggcaccgtg cgcacattca gcggtgattt aatcagtgag gccagcggca
     1861 cgctcacttc cgccgtctgc gccagcagtg cagacagggc agtctcctgc tcttttgacg
     1921 gtttacggct ggatttacgc tccgtttttg tcttagactc tgtaactgac atagttagta
     1981 cctcccaaaa cggtaataat gattgtctgc cggacgcgtt cccgtccggc acctctctta
     2041 cttaagaatc ccgaaatcac ggatgacatg attcatccag tacccggcac cgtcctcaaa
     2101 attccaggtg cgccagacca ttgcaccgtt attacggaca accaggcgaa actgttctcc
     2161 gtggtcatcc tcaataaaga cgttccggta ctgtgcggca acggcttcgg catgtttgcg
     2221 ggtaaaagga ccgtcaggca gtccctgaag tattctgaaa tattcgctca tcctcatctc
     2281 tccggtgatg ccccgcgcag ggcggggcgg ttatcacatt cagcccgatc agaacgggat
     2341 atcgtctgaa aacccgtaat cgtcaccctc cggcggttgc ggctgaggct ccggctgcgc
     2401 ggccttacgt ccacggcctt ttgttttcgc gctccctttt ttcgtcgtgt cagcactctg
     2461 tggctgacca ttctgctgag gctgcggttg cgcctgagcg ttctgctgtg gtgcactccc
     2521 caacatctgc acggtgcccg tggtcttaac aagaatttca gtgacgtaac gggtgatacc
     2581 attatcctcc cagctacggg tgcgaagctg accttcgatg tagacctgcg cgcccttgcg
     2641 cagatattca cctgccactt ccgcgagctt gccgaacagc accacgcgat gccattctgt
     2701 ctgctcccgc atctcccccg tctgtttgtc acgccagctt tctgacgtgg ccacctgcag
     2761 gtttgccact gcgcccccgt tggggatgta acggacttcc ggatcttttc ccagacgccc
     2821 gacgagaatg accttgttaa tgccacgaac tgccatgatg tatctccttc tgttgatgtt
     2881 ttcagccccc atcatctttt gagtttcggg agccgcggtt ttgacgtttt gcggggacct
     2941 gctctttgtt ccctccggta ttcaccatca cctttttgag aaccttcgcc gcaaggggca
     3001 tcactgccac ggccggaaac gactgtccgg gggcagaccg caaaagtttt tgcctgcttt
     3061 tgggcaaaaa gttttgcggg atgtacattt cacccctgga ctggcggaga cggttgtggc
     3121 tgtatcagcc cccggcgaca ggggcttaaa caggtgaatg gcaccggaca gaacaacggg
     3181 cagggacccg ccgcaacgac gtgaagcgcg gtttgcgaaa cggcgttgcc ccttacatcc
     3241 cggcggcctt ttgccgggat gccgggagcg caggccacgg cgcgacagag gcggcgcgac
     3301 cggaacggtc gctgcaagcc cggggtttcg cgtcagggat ggaagcccgc cagggcggag
     3361 acagcttcgc tggctccgtt cacgacagcc cgccccgcca gggggacgcc cggaaaagcc
     3421 ataaacagga gaggaacgga aagaggaaga agaagaagaa gaagaagaag aagaagaaca
     3481 gtaacaggca cggtaaccac agaaaaataa catcagaaaa aaacattccg gacacatcac
     3541 aaacgggaag ggtaacgggc gacgcgccag cgtggcccgc ttcccttccg gcaggcgtca
     3601 gcctgccagg ggttttcgcg cgggctgaca ggcggcctcc accgcctgcc ctgtatcacc
     3661 tgtttctgct gccctgaagg atatagtgac caccgaggaa aaccgcctgc tgattcacca
     3721 caaaatcgca gatttcatca acaatctgcg cagtggtgag cggtttctgc cccatttcac
     3781 gacgccgcgc attaatcacg gctttgtaat tgcgcagaat ttcgcgggtt tctgtgctga
     3841 tattcatgac atgttctccc cgtccagcgc ctgtaacaca acgtccatcg cttcgcgttc
     3901 ttccctgacg aatgcgtcac cgtcgccccc gttctcactc ccgacggcat acaggcgctt
     3961 catttcactg cctgccagcg ccatcacttt ttccagcatc accgccggga ctgtcacctg
     4021 acggctggcg cgcttttctt ccatcagttc acgaacaacg tcgtgaacaa cgctgacatc
     4081 cgcaccatgc tcaaaggcat cgtccagccg ctgcatgacg gtttccagct catcgtctga
     4141 caggttccag tcctgggcta tgtgacgcac atccccacgg gtcagcacca cggcaaaaac
     4201 gcattcactg tccggcacgt ctgtatccag cacttcgcgg ataatttctt taacagtaca
     4261 gtacatgttc tcgtctcctt tacaggggcg gcattgccgc cccgtccgtt aatcagtcca
     4321 gagccgcgta aatcgcgttg cattcagggt gaaattcgat gtgacgccac aactgcgcat
     4381 cgcgcatccg gtaaagctgg gtcagtcctg catccccgct gtcgtggtac agccacaact
     4441 ggcggttaat cacaagggaa gtcagaatta tgcctgcggc atcagcactc acggtacggt
     4501 caaaccagtt tgcaccgttc accatgtgga aacggtcacc gtccggcatc atgtaaccac
     4561 caccgtccgg caggcggatg aagtgccaga agccaccctg atattccggc atcatttttt
     4621 gcgccagcgc gtacacatac gcctcggcat acagaaaatc gttgccaaac aggtaaggca
     4681 gaaaagacag acggtcgcac tcttcaacat tcagagtgac aggtttcgca tactgcatga
     4741 tgttttcctc cgttgttcac atattccgcg acaccgggcg gtgccgtgcc tccataaaag
     4801 ccgggcgata aggcggggat gcaggggcgc aagggaagcc gcagcctgac gcactcggct
     4861 gacgggagtt ggagggtgaa accgcgaaat ctgagcgggt gaacgaccgg aaaatcacgg
     4921 cagacgggaa ggagggaccg ggtgaccgca gcggttcacc ttaccttgca acccgcccgc
     4981 agaagcccgg ctacga
//
LOCUS       DBBGLQ010000204         4989 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.204, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000204 DBBGLQ010000000
VERSION     DBBGLQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.204"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(235..813)
                     /locus_tag="ACIYSB_005182"
     CDS             complement(235..813)
                     /locus_tag="ACIYSB_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGC5432014.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYISGGTSGN
                     MFNQLLVSSKITKTAINDALRRMKKSGITKPELEAYLREILNSKNKSSLAFCSDDEGL
                     KIDGVIASVLMNNDYRSLYGVIVGRYRLRKSKLQMANELHIKHPDWALITCRRRVDTW
                     ISLAESLLYVPLCDAFGTNSDRFKLQSEQESA"
     gene            complement(822..1187)
                     /locus_tag="ACIYSB_005183"
     CDS             complement(822..1187)
                     /locus_tag="ACIYSB_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGC5432015.1"
                     /translation="MTESIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQRVPGGRLGVKIYKIESE"
     gene            complement(1188..2246)
                     /locus_tag="ACIYSB_005184"
     CDS             complement(1188..2246)
                     /locus_tag="ACIYSB_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGC5432016.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNPRVLVEPEPKSMCG
                     LPSGVVPAVRQPLAEDKSLLPFFSDDRVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SEIVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSLYRESDIVPGEQTVTSILKQR
                     TKNIALPPLVHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            complement(2248..2520)
                     /locus_tag="ACIYSB_005185"
     CDS             complement(2248..2520)
                     /locus_tag="ACIYSB_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432017.1"
                     /translation="MAHNTKNHNSDAVRASRHGRRSRVFKTDWYQHPPCTEEQAEWLI
                     QCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTYQQRIWR"
     gene            2656..2913
                     /locus_tag="ACIYSB_005186"
     CDS             2656..2913
                     /locus_tag="ACIYSB_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGC5432018.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            2919..3218
                     /locus_tag="ACIYSB_005187"
     CDS             2919..3218
                     /locus_tag="ACIYSB_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGC5432019.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKARSVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGVYHGGQNYESNIL"
     gene            3281..3634
                     /locus_tag="ACIYSB_005188"
     CDS             3281..3634
                     /locus_tag="ACIYSB_005188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432020.1"
                     /translation="MNRIIILLFSFAISGCVNPYNYREEQDIIISFKTNSPPQEIQEC
                     ILSEWQRVPLLYTITSQKIGKYYSVLAVADNADIYKSADGSTTIDFYSLRGVLDPTNG
                     KNKRIQGIKSCISNK"
     gene            complement(3739..3894)
                     /gene="hokD"
                     /locus_tag="ACIYSB_005189"
     CDS             complement(3739..3894)
                     /gene="hokD"
                     /locus_tag="ACIYSB_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGC5432021.1"
                     /translation="MKQQKAMLVALIVICITVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(4131..4796)
                     /locus_tag="ACIYSB_005190"
     CDS             complement(4131..4796)
                     /locus_tag="ACIYSB_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HGC5432022.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT         1254 a         1268 c         1138 g         1329 t
ORIGIN      
        1 ttgcagtacg cgcggcggtc agatacatga ctagtatttt cactgtcgcc tatctgctag
       61 ctcgccattg agcttcacca caacgataag agcactgcgc ggcacctttc accagttccg
      121 cgaggtctgc gggttcagtg ctcttacctg ttgtgcagac aaaaaaaccc gcacaatggc
      181 gggttcttac tattatgttg ctcagttcgc ttttcgtccc gagcttatca caattcaagc
      241 actttcttgc tcactttgca atttaaatct gtcgctattt gtgccaaacg catcacatag
      301 tggaacgtac aggagcgatt cggccaaact tatccatgtg tcaactcggc ggcgacaggt
      361 aatgagcgcc cagtcaggat gtttaatatg aagctcattg gccatttgca acttgctctt
      421 acgcagacga taccggccaa caatcacacc gtatagagaa cggtaatcat tattcatcag
      481 caccgaggcg atcacaccgt caatcttcag gccttcatcg tccgagcaaa acgccagact
      541 acttttgttt ttactgttga gtatttcgcg caaataagct tcgagctctg gtttcgtaat
      601 gcctgacttt ttcatacggc gcagcgcatc gttgattgca gttttggtaa ttttgctgga
      661 tacaagcagt tggttaaaca tgttaccgct agtccctcca ctgatatacg accaacgccc
      721 ccacatccgt aatttcccct ggatccagac ggcttccagc gtttttagac gtaaatgctc
      781 gccgcttttg cctgtaattt ccgggtatat catatttacg atcactcact ctcaattttg
      841 taaatcttca cgcccagccg ccccccagga acgcgctgac cgcgcacaat attgatttca
      901 tcaaactgct cgtcgtctat gagaagtccg gcatgcgtca gcgcatccag tggtgctttc
      961 aggatattgt ccaggtcacg acgacgttta tccggtggct ctgcaataat ctttatcgcc
     1021 agccgtccgg acaggtttaa cttcagccgc tgctggcgaa caatgagcgc cacatcacgg
     1081 cgataacgct caccggcttt tgatacaaaa tatgtgctgc cacgacgtcg ccagtaggtg
     1141 ttcaccgttg gcgggtaagg caaaacaaat tctatgcttt cagtcattta tgctttccac
     1201 ttcaggacac ctgaatttct cgcgtgcatt aaaaaacgaa tcagcaacaa cagctggctg
     1261 ccgtgttttt cttcaaaatc ttttaccccg gcgtgcagtt cgttatgaca tttacggcac
     1321 agcggaataa caaacaaatc atcagccttt gttcccatcc ctcccagtcc atgaccaatg
     1381 atgtgatgcg gatcatctgc ctgattaccg cacgtcatgc atttctgcgt ttttacccag
     1441 cgcgtgtata caggcatctc ttcccgttgt ggtttctggc gctggagata ctgagccggt
     1501 gactccggat caacggcaat gctgaccacc gtctcttcct gtggtgggtt ctgttgctgg
     1561 tggacgagag gcggtagcgc aatatttttt gtgcgctgct tcagtatgct ggtgacggtc
     1621 tgctctcccg gtacgatgtc gctttcgcgg tacaaggagc ggattttttc cgcacgtaac
     1681 cccagagaac gacgtaatac tgcctccgga agcgcgtccg ccacctgatt gcggaccgcc
     1741 caccaggata attcagccag agataattca cgctcctgcg taccgcttat tgcgtgaccg
     1801 atgacgtcaa tcatccatgc tgacaggttt tgatgagcaa gttgctcgag tgattcggat
     1861 gtctggtcac gcagctggtt gtcgcagtgc cagcacaaca ccatcgcgcc ggtaccgtaa
     1921 cgatgtatga cgatttcact gtgatgatag tcaccatgag gccactggca ggatttgaca
     1981 tgacgcaaca gccagtcaga cagtgcccca gcgccgccaa cagcacgaat cacccgatca
     2041 tcgctgaaaa atggcagtaa tgatttatcc tccgccagcg gctggcgaac ggcagggacg
     2101 actccggatg gcaggccgca catacttttc ggttccggct ccaccagcac tcgaggattg
     2161 tgaaataccg gcatggattc acgaccgggc ttaaggacca ccagcccgag ttccggtacc
     2221 agaacaggtc gaagtaatac ccgcacgtta cctccagata cgttgctggt atgtgcggga
     2281 cggacgcggt gggcgttctg agtaaggaag cctgacggag attatccagt gacgataatc
     2341 gaggctgagg gctttcttaa tctcgtatcc gtgtctgcgg taacactgaa tcagccattc
     2401 ggcctgttct tcagtgcatg gagggtgttg gtaccagtcg gttttaaata cgcgcgaacg
     2461 ccgcccatgc ctgctggcac gaacggcatc agaattgtga tttttggtat tgtgcgccat
     2521 ttgttttctc tgctggcgca gcaggtgcca gttgttcagg ctggcgtgcg gcaatattgt
     2581 ctctgatttc tgttgtcgtc aacaggcagc gtgctatcat cgaatagtgt tctatcctac
     2641 tccgtgaggt ttaccatgcg tacaacccaa caattcagca ttacattaac taacgagatg
     2701 gctgacatgg tgcgcgcccg tgtggcttcc ggtgcctatg cttcagaaag cgaggtcatt
     2761 cgtgaagggc ttcgtgcact gaatgagcgc gataaagcaa tcgaagcgtg gttaacgcat
     2821 tcagccgccc cctctcttga ttctatccgc gaaaatccaa acaacggacg ctccatttca
     2881 caggttcgcg ccgcgattcg atccgggaag taatctgcat gacatatgaa gtcatcatta
     2941 cccccgaggc cgaacagcag ataataaacc tgcacagata tataacggag aaagcaagaa
     3001 gcgtcattgc tgacaattat gccaatgcgc ttcttgatta tcttgatggg ttttctacat
     3061 tcccgcatcg gggcaataaa cgcgatgata ttcgccaggg gatgcgggta actcatttcc
     3121 gacacagaac gattattgct tttgccgttg atggcaataa agtctttatc gctggcgttt
     3181 accacggcgg acaaaattat gaatctaata ttttatagtt aatgcttata acacaccgca
     3241 actaaaataa ttgagtaata ttttcaaaag aggcaaaaaa atgaacagga tcattattct
     3301 tctgttttct ttcgcaattt ctggatgcgt aaacccttat aattatagag aagagcagga
     3361 tattatcatt tcatttaaaa caaattctcc accacaagaa atacaagaat gcattctcag
     3421 tgagtggcaa cgtgtacctc tcttgtatac tatcacttct caaaaaattg gtaaatatta
     3481 cagtgtatta gcagttgcag ataatgccga catttataag tcagcagacg gctctaccac
     3541 tattgatttt tattctcttc gaggtgtgtt agatcctacc aatggaaaaa acaagaggat
     3601 tcaaggaatt aaatcatgta ttagtaataa ataaaaatca ggacaaacta aggaacagcc
     3661 gaagcgggtt aagtgcgggt gcgttgagaa tgcctgacac atcagaggtg gcgagggatt
     3721 tctccctcgc ctggtctctt actcctcagg ttcgtaagct gtgaagacag cgacctccgt
     3781 ctggccggtt cggattcgta cctcgcagag gtctttcctc gttaccagtg ccgtcacaat
     3841 gacggtaata cagatgacga tcagggcgac taacatcgcc ttttgctgct tcatagcctg
     3901 cttctccttg cctttcggca cgtaagaggc taacctacat ttgctagaca tagattctgc
     3961 ctcagattaa tgttaagcgt cttgcaggac gcataatgtt aactggggct tttctctatc
     4021 tgccgttggt gttcatgccc gaggcagata gcctcaagca cccgcagcaa ttctacttaa
     4081 ctcccgtcac ctcgccaata tgaaatcaat cagaaaggtg atccataaaa tcactccttc
     4141 tcttcttttc cgtagtggag ttggccaatt tttataagag ggcgtccctg agatttgcgg
     4201 tgtagattgg tatcgcgcag agaatacaca cagccacaat attcctgctg atagaatttt
     4261 tcgcgcttgc tgatttcaat catacgggac gagccgccct gcttgcgcca gttataatcc
     4321 cagtacacca tacccggata atgcgcaaca gctcgccgcc cacactcgtt aacctgctgc
     4381 atatttttcc agcgtgaaat gcccagtgaa ctgctgatca cactgaaacc attttcagca
     4441 gcgtacaacg ctgtccgctc aaaacgcatg tcaaaacaca tggtacaacg gatccccctc
     4501 tcaggctccc attccattcc tttggcacgt tcaaaccagt tgtcggtgtc gtaatcagca
     4561 tcgataaacg gcacgccgtg ttgttcagca aagcgaatat tttcatcctt acgaattaaa
     4621 tactctttct gaggatgaat gttcgggttg tagaaaaaga tggtgtagtc gattcccgag
     4681 gcctgaagcg cctccatcac ttcaccggaa catggagcac agcaagagtg cagtagtagt
     4741 ttgtttgccc cgttcgggag ctccaattta ggccgtttga aatcagcaat agtcataaat
     4801 atttttattg ggggtcatga aaatagcaca gagtgtagca tcagagcagg gctatcggga
     4861 atatatgtct aaatctggta atatctggtt ttgacgcaaa gcggacaacc acactagctc
     4921 taccctgcgc catgaaaatg tcaattcaca tctgaactaa tgctctttaa tctagtaacg
     4981 tctaaaata
//
LOCUS       DBBGLQ010000205         4982 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.205, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000205 DBBGLQ010000000
VERSION     DBBGLQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.205"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..205
                     /locus_tag="ACIYSB_005191"
     CDS             <1..205
                     /locus_tag="ACIYSB_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GspL/Epsl periplasmic domain-containing protein"
                     /protein_id="HGC5432023.1"
                     /translation="TLLLEHPELQLMEANYSQKQKSLTLKMSAKSEANIDRFCELTQS
                     WLPMEKTEKDPVSGVWTVRNSGK"
     gene            202..663
                     /gene="gspM"
                     /locus_tag="ACIYSB_005192"
     CDS             202..663
                     /gene="gspM"
                     /locus_tag="ACIYSB_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HGC5432024.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            663..1340
                     /gene="gspO"
                     /locus_tag="ACIYSB_005193"
     CDS             663..1340
                     /gene="gspO"
                     /locus_tag="ACIYSB_005193"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HGC5432025.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(1369..1845)
                     /gene="bfr"
                     /locus_tag="ACIYSB_005194"
     CDS             complement(1369..1845)
                     /gene="bfr"
                     /locus_tag="ACIYSB_005194"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGC5432026.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(1917..2111)
                     /gene="bfd"
                     /locus_tag="ACIYSB_005195"
     CDS             complement(1917..2111)
                     /gene="bfd"
                     /locus_tag="ACIYSB_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGC5432027.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(2280..4973)
                     /gene="chiA"
                     /locus_tag="ACIYSB_005196"
     CDS             complement(2280..4973)
                     /gene="chiA"
                     /locus_tag="ACIYSB_005196"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HGC5432028.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKSNDASNPWRYVRAATATEISETSNPQSCTSAPQPSPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEISQYGNPGSCSVKPDNNGGAVTPVDPTPETPVTPTPDNSEPSTPADSVND
                     YSLQAWSGQEGSEIYHVIFNGNVYKNAWWVGSKDCPRGTSAENSNNPWRLERTATAAE
                     LSQYGNPTTCEIDNGGVIVADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGD
                     NPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPW
                     KPLGKAQSYSNEELNNAPQFNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRV
                     FVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKD
                     ANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQH
                     YYDIVDNLNLKVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPIL
                     PTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIAN
                     LHSQLKGLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVG
                     IWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         1228 a         1273 c         1142 g         1339 t
ORIGIN      
        1 aacacttctg ctggaacacc ctgaactgca gttaatggag gcgaactata gccagaagca
       61 aaagtcgctg accttaaaaa tgagtgctaa gagtgaagct aatatcgatc gtttctgtga
      121 gttaacacag tcctggcttc cgatggaaaa aacagaaaaa gatccggtca gtggtgtatg
      181 gacagtaagg aactcaggga aatgattaaa tcatggtggg cagaaaaatc cacatcggaa
      241 aaacagatcg ttgcggcact ggcggttctc tcgctcggcg ttttttgttg gcttggggtg
      301 ataaaaccga tagataccta cattgcagag catcagtccc acgcgcaaaa aattaagaag
      361 gacatcaaat ggatgcagga tcaagcgagc acccatggtt tactcggtca tcctgcgctt
      421 acgcagccca tcaaaaatat ccttctcgag gaagcaaaac gtgagaattt ggctatcacg
      481 ctggaaaacg gcccggacaa tactctgaca attcatcctg tcacagctcc cctcgaaaac
      541 gtctcccgct ggctcactac ggcacaggta acatacggta ttgttattga agaccttcag
      601 tttactcttg ccggaaacga agagatcacc ctcaggcatt tgtccttcag ggagcaacaa
      661 taatgacaat gctactgccg ctcttcattc tggttggttt tattgcaggt tattttgtta
      721 atgccatcgc ctatcacctc tcgccgttgg aggataaaac ggcgttaacg tttcgccagg
      781 tattggttca tttcaggcaa aaaaaatatg cctggcatga tacagtgccc ctgatacttt
      841 gtgttgctgc tgctatcgcc tgcgccctgg cacctttcac gcccatcgtg actggcgcac
      901 tctttctcta tttctgtttc gtactcacgc tcagtgttat tgattttcgc actcagctcc
      961 tgcccgacaa actcacctta ccgctgctct ggcttggctt ggtatttaat gcgcagtatg
     1021 gattaattga tttacatgat gcggtttacg gcgcggtagc gggctatggg gtgctgtggt
     1081 gtgtttactg gggcgtctgg ttagtttgtc acaaagaggg attgggctac ggtgatttca
     1141 agctactggc cgctgcaggc gcatggtgtg gctggcaaac gttgccaatg atactgctga
     1201 ttgcctcgct gggtggcatt ggttacgcca tcgtttcaca acttctgcaa cgccggacta
     1261 taaccactat cgcattcggc ccgtggcttg cgctcggcag catgataaac ctggggtatc
     1321 tggcctggat ctcttattaa ccgggagggt tctccctccc gacacggctc aaccttcttc
     1381 gcggatctgt gcttgcagat aattttgcag gcccatcttc tgaatcagat caagttccgt
     1441 ttccagccag tcgatatggc cttcttcatc acgcaaaatt tctatcatca tatcgcggct
     1501 gacgtaatca tgaacgctat cggcataacc aattgcctca cgcaaattct tcgcgccatc
     1561 cagctcaagt gccagatcag aacgcagcat ttcctcaaca tcttcaccaa tgttcagttt
     1621 gcccaggtcc tgtaagtttg gaagaccttc cagaaaaaga atgcgctcaa tataacgatc
     1681 ggcgtgtttc atctcatcaa tggattcatg atactccaca tcattgagac gtttgagacc
     1741 ccagttttta aacattcggg catggagaaa gtactgattg attgcgacaa gctcatttcc
     1801 caacagtttg ttgagataat ttataacttt agtatcacct ttcatttttt gactccttcg
     1861 cttccactct ttgaagagta gaacggctta cgagtaagtc aaaaaacaca ctccgtttat
     1921 gcggactcct taaactccgg cagctgcatt aattcatcct ccatcacttc acgcgcggcc
     1981 cggacacatt taccgcactg attgccgacc ggaataaatt tttttaattg ctggaacgag
     2041 tgaggggaaa actggcgcac agcctgacga atttttttat cgctgatacc attacaaaga
     2101 caaacgtaca tagagactcc cgtatacttt cttcaaaaat aaatgaaaat agttcttatt
     2161 tcaattacgg aaagactctt ttttaagcaa agaatagaaa tatgaataaa aataatgggc
     2221 taccaaaagt agcccattga caaaaaatgc ggcgatactg gaaggtatcg ccaacacatt
     2281 tattgcttag taaacggcgc gaagatttcg ctaaatgcgt acttcggtgc ctgttcttta
     2341 gtcaggccgt ggaattccgg agacaggtta gtgccgcccg gcaggtcgcg cgcgattgac
     2401 cagataccaa ccataccgag attacgcttc tgcgcatcct gcatgaccag acgagcatca
     2461 gagagataga acacctcgcc ctgaacgtcg ttcacgccaa ccatcggcgt ggtacccatc
     2521 atagcgtcaa tttctgcatc gctcttattg ggatggaggc ctttcaattg tgaatgcagg
     2581 ttggcaatcg cagacgttgc acacttaccg tgaatgttct ggccttcggt atttgcagac
     2641 tgacaaatcg cgttaccgta gtccattgtc atcacgttca cacccgccag ctcaacacct
     2701 ttcgctttgg catcgctcag gacattcatc ccttccggcg tcaggccagt cggcagaatt
     2761 ggcaaggtgt accagatagc aatatctttg ccttctgact tccatttatc ctgcactttc
     2821 ttcacagcaa ggttacgacg ttcaatagat gcctgatccg caacccaggt gccttcgata
     2881 tcgaagtcca ggactttgag gttcaggtta tcaacgatgt cataataatg ctgcatcaga
     2941 tcgtctacgt tcttacagga agcagccagc ggagcgttgt tagcaccacc gatagacagc
     3001 atcacatcgc cgccagcctc acgcagagct ttgattttgc tgtactgagc gtaattctgc
     3061 ataccgtaag cggtacccca tgtcggcaga caggtgttcg catctttact caccacaaac
     3121 gccagcgtga agtggttgac gttatgattc ttagccagcg cagccagatc cgggatcgta
     3181 ttcagcgtaa agtcgacata cggtgcataa acgtgtttcg gccacgcttt cttcggcgta
     3241 cctacgcgct ctttggttcc ggtccagtca acaaaaacac gccacgggtt gctgtcagaa
     3301 ggagaaactt tctgcacttt actctgagaa atgtagttca caccgttaaa gcgaatcagc
     3361 gtatcgctgg cataaagcgt ttctggatta aactgcggcg cattattcag ctcttcgttg
     3421 ctatagctct gagccttacc taacggcttc cacgggcggc tattgctacc ggtggcgttt
     3481 tggttcgcca ccagagcagg gttgtcactt tgcgtccaga acagcgcttc ataagcctgc
     3541 ccatcaacaa caacgcgatc acctttcacg tacacagtgg atgcggacca tgctttcgcc
     3601 ggttcgtatt tcttccacgg gttatcgccg cccgggacaa agccccatgc gtcttgatcg
     3661 acagaagttt tataatgtgc atcgttataa tctacgatgc tgtccgcgct gtacgctttg
     3721 ctggcctgga aaccatccgc aacaatgacg ccgccgttat caatttcaca ggtagtcggg
     3781 ttaccgtact gactcaattc cgcagcggta gctgtacgct cgagacgcca tgggttattg
     3841 gagttttcag cgctggtacc ccgtgggcaa tctttagacc caacccacca ggcgttcttg
     3901 taaacattac cattgaaaat aacatggtaa atttcgctac cttcctggcc gctccacgct
     3961 tgcaatgagt aatcgttaac gctatccgct ggtgttgatg gctcgctgtt atccggagtt
     4021 ggggtcaccg gtgtttccgg agttggatca accggagtca cagcaccgcc attattatcc
     4081 ggcttaacgg aacaggagcc agggttacca tactggctga tttctgttgc cgttgccgcg
     4141 cgaacataac gccatgggtt actggcatcg ttttctttcg catcgcctgg gcaatttgcc
     4201 gcgcctaccc accaggcgtt cttataaatg ccgccgttat agataacgta ccatgtagaa
     4261 gaaccttcct gacctttcca ggctactaca gcatagttgt cgtttgactt atcagctggg
     4321 gctggctgaa catcaggagc cggtttaacg tccggtgccg gtttcacatc cggtgaaggc
     4381 tgtggtgctg aagtacagga ctgtggatta ctggtttctg agatttccgt tgccgtagcg
     4441 gcacgaacat aacgccatgg gttgctggca tcattgcttt tcgcatcacc cggacagtta
     4501 gaagaggcta cccaccaggc atttttatat accgcaccat taaagacgac ataccaggtc
     4561 tgaccgccct gctgtttatt ccaggcaact acagaagaat tagacggaac agtgccctgt
     4621 gctggtgtag ccgaaccgcc attagccggc gtattggatg caggcgtatt tgaatttgaa
     4681 gaagatgagc tgccgctctt ttcgcaggaa agtgtattgc caaactgact aatttcagca
     4741 gctgttgcgg tacgctttaa acgccacggg ttagttgcat catttgcttt cgcttttccc
     4801 gggcaatttg tagaagaaac ccaccaggca ttttcataaa ttttgccatc gaaaataacc
     4861 tgatatttat taccaccttg ttggttattc catgcttcca ttgccaatgc tggcagagcg
     4921 gaacacacca gccccatacc aatcatagat ttagtaaata tatttaattt cattataatc
     4981 cc
//
LOCUS       DBBGLQ010000206         4880 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.206, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000206 DBBGLQ010000000
VERSION     DBBGLQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.206"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..416
                     /locus_tag="ACIYSB_005197"
     CDS             <1..416
                     /locus_tag="ACIYSB_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5432029.1"
                     /translation="LAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWD
                     ALCYYSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGSERGALLYGLIGTCRLNG
                     IDPEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            574..705
                     /locus_tag="ACIYSB_005198"
                     /pseudo
     CDS             574..705
                     /locus_tag="ACIYSB_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1069..1167
                     /locus_tag="ACIYSB_005199"
                     /pseudo
     CDS             1069..1167
                     /locus_tag="ACIYSB_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1238..2530)
                     /locus_tag="ACIYSB_005200"
     CDS             complement(1238..2530)
                     /locus_tag="ACIYSB_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5432030.1"
                     /translation="MAHRYLTRQDHRTLSLAALGGALEFYDFIIFVFFAAVIAELFFP
                     PDIPEWLRQLQAFGIFAAGYLARPLGGIILAHFGDILGRKKMFTLSILLMAVPTLVIG
                     ILPTYSSVGTLAPVLLLLMRLLQGAAIGGEVPGAWVFVAEHVPERRTGIACGTLTAGL
                     TAGILLGSVVATLVNSVLSQDIVYDYGWRFPFLLGGVFGLAAMYLRRWLEETPVFLEM
                     KARKKLAQELPVKAVVLRHKKAVVISMLLTWLISAGIIVVILMAPVWLQKQYDFSPTV
                     TLQANSIATIMLSLGCLLAGMAIDSFGAGKTFISGSILLGCSGWLFYHTVGEYPEMLF
                     WLYGLAGFSVGIVGAVPYVLVRIFPAEVRFTGISFSYNVAYAVVGGLTPVVVTMLTEI
                     SPMAPAWYVMLLSLVGSGLGIWFCRDPRLFICHADKDK"
     gene            complement(2706..4349)
                     /gene="lldP"
                     /locus_tag="ACIYSB_005201"
     CDS             complement(2706..4349)
                     /gene="lldP"
                     /locus_tag="ACIYSB_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGC5432031.1"
                     /translation="MDIWSQDYDPVGNIWLSSLTASLPILFFFFALIKLKLKGHVAAT
                     WTVIIALIVALLFYKMPLTAALASVLYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRASVLSVTPDQRIQMLIVGFAFGAFLEGAAGFGAPVAITAALLVVLGFRPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGLDSFEIGQMVGRQLPFLTIIVLFWIMAIMDGLR
                     GIKETWPAVLVAGGSFAIAQFLSSNFLGPELPDIISSLVSLVCLTLFLKRWQPVHIFR
                     FDNTPYQNTDSSPLSRSQVIRAWSPFLFLTATVTLWSLPPFKTLFAPGGTLHDLVITV
                     PVPYLDKLIARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSVIWLKMKPSDAVS
                     TFGSTLKELALPVYSIGMVLAFAFISNYSGLSSTLALALAQTGSAFTFFSPFLGWLGV
                     FLTGSDTSSNALFASLQATAAHQIGVSDILMVAANTTGGVTGKMISPQSIAIACAATG
                     LVGKESELFRFTVKHSLIFTCIVGVITSLQAWVFTWMIP"
     gene            complement(4696..>4880)
                     /locus_tag="ACIYSB_005202"
     CDS             complement(4696..>4880)
                     /locus_tag="ACIYSB_005202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432032.1"
                     /translation="NTPVQAECIFMAYPFRRKCDIPHMTETTQRKTGADSRIQDKLTL
                     HNPALLRYNIHGQLPL"
BASE COUNT         1386 a         1196 c         1275 g         1023 t
ORIGIN      
        1 gtcttgccgt caggcagcag cgaagcaaag tgttactgac gtcgctgcat gaatggatgg
       61 tggagaagaa tggtacgctg tcgaaaaaat ccagactggg cgaagcgttc agctatgtac
      121 tgaatcagtg ggatgccctc tgttattaca gtgatgacgg tctggcggag gcggataata
      181 atgctgcgga aagagcgctt cgtgcagtct gtctcggaaa gaaaaacttt atgttctttg
      241 gcagcgatca cggcagcgag cgtggagcac tgttgtacgg gctgatcggc acctgccgtc
      301 tgaacggtat cgatccggaa gcgtatctgc gccatatcct gagcgtactg ccggaatggc
      361 cttccaaccg agttgacgaa ctcctgccat ggaacgtagt actcaccaat aaataagcgt
      421 caatacggtg ctccgttgac gcttacagcc atcaagacct ctactgagaa gaaaattcag
      481 cagtagagac tatgcagaaa agttaattcc aggagtgact gcaggatgcg aatcaatgat
      541 cggacaatgc gaatcagcga tcgagtacga tgcgaatcgg cgatcgaaca cgatgcgatt
      601 ttgcaacatg cctggctgac ggacgtactg acgcgcctgc cggagtggcc tgaggaccga
      661 ctggctgagt tgctgcctct tgagggattt accttatccg ggtgacacaa ggcctgccgt
      721 caggtctttg cacaccgggc catggcctgc tgtcgggcgt tgagcgcctg acagcgggtt
      781 attgagaaag tcagtcacca cgcgccctgg ctgacagtca gggcatgcac gtcagcccct
      841 cccttaccct ggtcaggact tcactgcctg ccacaaactc ccgtgtcagt acttcgtggc
      901 ctgacgcgct caccctactc agtacttcag agcccgcctc cggtacctgg caaggcagac
      961 gccggggcgc aataaaataa atcaaccatc gcagggcggc gatcattcgc aaaaaccgct
     1021 gataacgagc ccggaaacgt gtcaggacga tttcaccgga acatgacggc ggaccactac
     1081 ctggtgatgt cgcgtggcgc tatcatccgg cagggcaagg gggaggatat ggagactgat
     1141 ggggggcgcg gtctggtgag catctgaccg gatggcgaca ggttaacctg tcgccatttt
     1201 taaaaactga atgataccgt tatataacgg tgaaacatta tttgtctttg tctgcgtgac
     1261 agataaaaag acggggatcc ctgcagaacc atattcccag tcctgatccc acaagtgata
     1321 acagcataac ataccatgct ggagccatcg gggagatttc cgttaacatc gtgaccacaa
     1381 caggagtcag tccccccacc actgcataag cgacattgta agagaatgaa atgccggtaa
     1441 acctgacctc tgcggggaaa atacgcacga gaacataagg gacggccccg acaatgccca
     1501 cactgaatcc cgccagccca tacagccaga acaacatttc aggatactca ccgacagtgt
     1561 gatagaacag ccatcctgaa catccaagca gaatactgcc tgatataaag gtttttccgg
     1621 caccaaaaga atcaatggcc ataccggcca gcaggcatcc cagactcagc ataatagttg
     1681 caatgctgtt agcctgtaag gtgactgtcg gcgaaaagtc atactgtttc tgtaaccaga
     1741 ccggtgccat cagtatcacc acaataatcc ctgcggatat cagccatgtc aacagcatgg
     1801 aaatcacaac agctttttta tgtcttaaaa cgacagcttt taccggtaat tcctgagcca
     1861 gtttttttct ggctttcatt tccagaaata ccggagtttc ctccagccag cggcgcaggt
     1921 acattgctgc caggccaaaa acaccaccaa gaaggaaggg gaatcgccag ccgtaatcgt
     1981 aaacaatatc ctgagacaat actgaattga caagtgtggc aaccactgaa ccaagcagga
     2041 tcccggcggt taaacctgct gtcagtgttc cgcatgcaat accggttcgg cgctccggta
     2101 catgctcagc aacaaaaacc caggcaccgg gcacttcacc gccaatcgct gcgccctgaa
     2161 gcagacgcat cagaagaagc agcacaggag ccagggtacc cacagagctg taggttggca
     2221 aaataccgat aacaagtgtc ggtactgcca taagcaaaat actcagggtg aacattttct
     2281 ttcgcccaag tatatcgcca aaatgggcca gaattattcc gccgagaggt cgtgccaggt
     2341 acccggcagc aaatatacca aatgcctgga gctgacggag ccattccggt atatccggcg
     2401 ggaaaaagag ctcagcaatg acggcggcga aaaaaacaaa aatgatgaag tcatagaatt
     2461 caagtgcgcc tccgagcgca gccagagata aggtcctgtg atcctgtcgt gtcaggtagc
     2521 ggtgtgccat ataaacatac ctgtcagttg aatattgaat gtgaaaccct gtcgtcagta
     2581 tatccgtgac ttaagaaaac cagaatatcc ggcagtcacg tcagccagca ctactgcaga
     2641 gtgttataag ccagaggctg taacacacat catcatgccg gaaaagatcc ggcacaacaa
     2701 atatttcatg ggatcatcca ggtgaatacc catgcctgga gagaggtgat gactcccaca
     2761 atacaggtga agatcaggct gtgtttaaca gtaaaacgaa agagttctga ttctttgcca
     2821 acaagtccgg ttgctgcgca ggcgatggca atggactgtg gagaaatcat tttgccggtt
     2881 actccccctg tggtgttggc ggcaaccatc agaatatcgg agaccccgat ctgatgagct
     2941 gccgttgcct ggagtgatgc aaataatgca ttggatgagg tatctgatcc cgtcagaaat
     3001 acgcccagcc agcccagaaa cggtgagaag aacgtaaagg cgctgcctgt ctgtgccaga
     3061 gccagggcaa gtgtggatga aagccccgaa taattagaga taaaggcgaa agccagcacc
     3121 atgccgatgg aatagacggg cagggcaagt tctttcagtg tactgccaaa ggtgctgaca
     3181 gcatctgacg gtttcatctt cagccagatc acagagagca atgctgcaaa caggatggcg
     3241 gtaccggtgg cagagaacca gtcaaactta aacaccgcgg cataagcagt ggcttcgctg
     3301 actaccggag gcatacgtgc aatgagttta tcaagatacg gcacaggaac agtaatcacc
     3361 aggtcatgca gtgtaccgcc cggtgcgaac agtgttttaa agggaggaag actccagagt
     3421 gtcaccgtgg cagtcagaaa cagaaatggc gaccatgccc gtatcacctg tgaacggctc
     3481 agtggtgatg agtccgtatt ctggtacggg gtgttgtcaa agcgaaaaat atgtacaggc
     3541 tgccagcgtt tcaggaaaag cgtcagacaa accagcgaca ccagtgatga tatgatgtcc
     3601 gggagctccg ggcccagaaa gttggaactg agaaactggg ctatggcgaa ggaacctccg
     3661 gcaaccagaa cggcgggcca ggtttcctta atgccacgca ggccatccat aattgccatg
     3721 atccagaaca acacaataat ggtcaggaag ggtaactgac gaccaaccat ctgaccgatt
     3781 tcaaagctgt ccagcccggt aacctggcct gcgacgagaa tggggatccc catcgcacca
     3841 aaggccacgg gagctgtatt gacgatcaga cacaacccgg ccgcgtaaag tggtctgaac
     3901 ccaagcacca cgagcagtgc tgctgttatg gccacaggtg ctccaaaacc ggcggcccct
     3961 tcgagaaagg caccaaaggc aaaccccaca atcagcatct gaatacgctg atcaggcgta
     4021 acggacagca ctgaagcgcg aatgatatcg aactgtccgg tttttacaga aattttataa
     4081 acaaaaactg cggcaataat tatccaggca atgggccaca ggccatagaa gaaaccatag
     4141 agcacggaag cgagtgcggc tgtgagaggc atcttataaa aaagaagggc cacgatgagc
     4201 gcgataatga cagtccatgt ggcagcaaca tggcctttaa gcttcagctt aatcagggca
     4261 aagaaaaaaa acagaatggg aagcgatgct gtcaggctgg acagccagat atttccgacg
     4321 ggatcatagt cctgtgacca gatatccatg aacagacctc ctggtgaagt atggtgtaat
     4381 acctgtacag cagagagcga gtgcattgct gacatgtccg tcagagtgtc tgcatattgt
     4441 ttcagtccgg caacgggagg aaggtcgtaa tttcaggatt tgtgaattat ccggagtaag
     4501 ggtgtaaatc tggtagtacc acaatctcat cgtctgtttt gttccggatc tttttaatga
     4561 aaggtgaaga aggatcttat acgacggtat atacgtattg attatatctt tatttataga
     4621 aattataaaa aaatgaaaaa aagccgtaca ccacagacgt acggcacaca aatacccaaa
     4681 ttaaggaagt tttcgttaca agggcagttg cccgtgaatg ttgtagcgta ataatgccgg
     4741 attgtgaagt gttaatttat cctgtatccg gctgtctgcg cctgtttttc tttgtgtggt
     4801 ctctgtcatg tgcggaatat cgcatttacg cctgaatggg taagccatga aaatacattc
     4861 tgcctgaacc ggagtgttgc
//
LOCUS       DBBGLQ010000207         4741 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.207, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000207 DBBGLQ010000000
VERSION     DBBGLQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.207"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(84..845)
                     /gene="kduD"
                     /locus_tag="ACIYSB_005203"
     CDS             complement(84..845)
                     /gene="kduD"
                     /locus_tag="ACIYSB_005203"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGC5432033.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(875..1711)
                     /gene="kduI"
                     /locus_tag="ACIYSB_005204"
     CDS             complement(875..1711)
                     /gene="kduI"
                     /locus_tag="ACIYSB_005204"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGC5432034.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1998..3179)
                     /locus_tag="ACIYSB_005205"
     CDS             complement(1998..3179)
                     /locus_tag="ACIYSB_005205"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGC5432035.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLAEVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3434..4663
                     /locus_tag="ACIYSB_005206"
     CDS             3434..4663
                     /locus_tag="ACIYSB_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGC5432036.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1232 a         1209 c         1041 g         1259 t
ORIGIN      
        1 cataaccatg atatggatta tgatgatcta caggtataaa aaaccctgcc atgcggcagg
       61 gtcataaaag taagaagaat gaattaacgc gccagccaac cgccatccac ggcaatggta
      121 taaccattca cataatctga agcgctggag gcaaggaaca ctatcggccc catcaggtca
      181 ctcggcagtc cccaacgacc agctggaatg cggtcgagaa tttccgcgct acgttgttca
      241 tctgcccgta gttgttgagt attgttggtc gccatgtaac ccggggctat cgcattaaca
      301 ttaatgttgt gtttagccca ttcgttcgcc atcaatcgcg tcacacccat cacgccgctt
      361 tttgatgcgg tataagaagg cacacggatc ccgccctgga aggagagcat tgacgcgata
      421 ttgataatct tgccgccatt gccttgcgcg ataaagtgtt tcgccgctgc ctgagacatg
      481 aagaatacgc tcttgatatt caggttcatg acatcgtccc agtccttttc gctgaactcg
      541 agagcatctt cgcggcgaat caatccggcg ttattcacca ggatatcaat atgaccaaac
      601 tccgctaccg cgcgatccag cagtgctgga ataccatcaa tctttcgcag atcggcggtc
      661 aggcttaaaa aacgacgccc cagcgctgtg acctgctcga tggtttcagt cggttcaacg
      721 atgttaatgc caacaatgtc acagcccgct tgcgccagcc ccaacgccat cccctgaccc
      781 agtccagtat cacaaccagt gacgaccgca actttacctt cgagagaaaa tgcacttaaa
      841 atcataacaa taccttattc gtttatgccc acaactagcg caaatcttta acggccacat
      901 ggtccatatc atcaaagacc tggttttcac cgaccatgcc ccagataaag gtataagctt
      961 tggttccgac accggaatgg atcgaccagc tcggggagat caccgcctgc tcgttatgca
     1021 tcacaatatg acgcgtttct tgcggctgcc ccatcatgtg gaaaacgcag gcgtcatcat
     1081 ccatattgaa atagaaataa acttccatcc ggcgctcgtg ggtgtgacac ggcatggtgt
     1141 tccacaagtt acccggagcc agctccgtca gccccatact caattggcag gtttccagta
     1201 catccgggac aaaatatttg ttaatcgtgc gacggttact ggtgaggtta tcgcctaacg
     1261 tgactggaga tacttcgtcc ggtgtgactt ttttggtggg atacgtcgta tgcgcgggtg
     1321 cgcaattgta ataaaacttc gccggagtgc cggtatcaat actggcaaag acaacttctt
     1381 ttgcaccttt accaacatac agggcgtcgc ggtgaccgat ttcatagcat tggccatcga
     1441 cagtaatcgt accggcaccg ccaatattga taacacctaa ctcgcgacgt tcaaggaaat
     1501 agcttacgcc gagttgttta ccaacttccc cgccaacgga aaccgtttta gttatcggca
     1561 taatgccgcc aacaataatt cggtcaatgt ggctgtaaac catggtgtac tcatcggcga
     1621 caaatacctt ttcaaccaaa aattcattgc gcagcccttg ggtatccagc gtttttgcgt
     1681 gcgcactgtg gatgctctgt cttacgtcca catcaacctc cgataatcag tgaagcgtga
     1741 aaatagaaaa cgatagtgaa acactgtttc gttttctcta taaagggatg atatgcccat
     1801 gggctagggt tttcaattaa aaataaaaca atgtttcatt tttaagttaa ggattaaaaa
     1861 aagtgcagtg tcgatcacga atatgtcatg agaatggaaa aatgagggag cccagaaaat
     1921 gtatgtcgac ttgccagcca tcattattca acagattaat gacggcagat atttatcggt
     1981 aaggaggaaa tgcagagcta ttcgtcacgt tcaatggtca atgccacacc ctggcccccg
     2041 ccgatacaaa gcgttgccag tcctttgcgg gcattacgtt tcaccatttc atgaaccaga
     2101 gaaaccagga ttcggcaacc ggaagcgcct atcgggtgac cgagtgcgat cgcgccacca
     2161 ttgacattga cccgacgctc atcccactca agcatcttgc caaccgaaag cgcctgtgca
     2221 gcaaacgctt cattagcctc gataagatcg acttcagcca actgccagcc tacacgctcc
     2281 aggcaacggc gggtcgcata caccggcgca attcccatca atgccggatc tacaccaacg
     2341 ctggcaaatg cgcgaatgcg ggccagcacg ggtaaattca acgctcgtgc tttggcttcg
     2401 ctcatcatca ttaccgcagc tgcgccatcg tttatggatg atgcattacc cgctgtcaca
     2461 gaaccgagac tatcaaatga aggatttaaa cgggctaagc cttctgcgct ggcgtcagtg
     2521 cgtggctgtt catcggtatc aacaaccaac gtctgcccgt tactttgggt cattaccggg
     2581 acgatctcat ctttaaatcg tccggcgtca atcgccgctc gcgctttttg ttgcgagcta
     2641 agtgcgtaag catcctgcaa ctgacggctg atgccatatt cgcgagccag attttcggcg
     2701 gtgacaccaa tatgataatc attgaaggca tcccacaacc catcatgcac aagactgtca
     2761 accaactggc tattgccaag ctgtgcaccg gtgcggctat cagtcagaac atgtggtgcg
     2821 cggctcatgt tttcctggcc accggcgatg acaatatcag cctcgccaca ctgtatcgcc
     2881 tgagtagcca gatgcagtgc tttaagcccg gaaccgcaaa cgtcattaat agtgattgca
     2941 gaaacgctat taggcagacc acctttaata gccgattgcc ttgccggatt ctgccctgcc
     3001 cctgcagtca acacctgacc aagaattact tcatccaccg catatgcagg aacgccggta
     3061 cgttctatta acgctttcac gaccagacta ccaagttcca cggcggaatg acccgctaac
     3121 gcaccacgaa agcagccgat aggtgtccgt aacgccccga caatcacaac gtctttcatc
     3181 acaacctcgc accaattaac agtgcaatag taaatgattg ttaattacag ttatcttaat
     3241 tgtttaaaaa aagtgatttt tatcacaaag gaaatatgcc tgagcagcag tcagagacat
     3301 aactggcacg taaggtttgc aaccactaac ccaccaatag aggggtagat agggcgttaa
     3361 tctcccatac ttaacctggt ttatggtaaa ttgccctcca ttttgtttaa tttgtagatg
     3421 atacgttcag ataatgtcta atatttggtc taaagaagaa actctgtgga gtttcgcgct
     3481 ctacggcaca gccgttggtg caggcacgct cttccttcct attcagttag gttcggcagg
     3541 ggctgtggtc ctgtttatta ctgctctggt cgcctggcct ttaacatatt ggccacataa
     3601 agccttgtgc cagttcatcc tctcatcaaa aacatcagca ggtgaaggga taacgggcgc
     3661 ggtaacacac tactatggca agaagattgg taatctgatt accacgctgt acttcatcgc
     3721 cttttttgtc gtcgtgttga tatatgcagt ggcaattacc aactcactta cggaacagct
     3781 ggcaaagcat atggttattg atcttcgcat ccgtatgttg gtgagtctgg gtgttgtatt
     3841 aattctgaat ctcatttttc tgatgggacg tcatgccact attcgggtaa tgggattttt
     3901 ggtattccca ttgattgcct atttcttatt tctttccatt taccttgtcg gtagttggca
     3961 acctgatcta ttaacaaccc aggtagagtt caatcagaat acccttcacc agatatggat
     4021 atcgattccc gtgatggttt tcgcctttag ccatacgccc attatttcta cgtttgccat
     4081 agacagacgt gaaaaatatg gcgaacacgc tatggataaa tgcaaaaaaa ttatgaaagt
     4141 cgcttatctc atcatctgca taagtgtact gttctttgtc tttagctgcc tgctttctat
     4201 tccaccttcg tatattgaag ctgctaaaga agaaggggtc accattttat cggcgctttc
     4261 tatgctgccg aacgccccag catggttgtc aatttccggg attattgtcg cagtagttgc
     4321 gatgtcgaaa tcattcctgg gtacgtactt tggcgttatt gaaggtgcca cagaggtcgt
     4381 caaaacaaca ctacagcagg ttggtgtaaa gaaaagtcgt gcatttaacc gcgcactatc
     4441 aattatgttg gtatcgctga ttaccttcat tgtttgttgc attaacccga acgcgatttc
     4501 gatgatttac gcgatcagcg gcccgctcat tgccatgata cttttcatca tgcctacgct
     4561 gtcaacgtat ctcatcccgg cgcttaaacc ctggcgttcc atcggaaatc tgattacgct
     4621 gatcgtgggt atcctgtgcg tatcggtaat gttctttagc taatatcagt tgtggatggt
     4681 tcaaaattgc tacaattttg aaccattcca tttttcataa tattcatttg ttattattcc
     4741 a
//
LOCUS       DBBGLQ010000208         4724 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.208, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000208 DBBGLQ010000000
VERSION     DBBGLQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.208"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..267
                     /locus_tag="ACIYSB_005207"
     CDS             <1..267
                     /locus_tag="ACIYSB_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGC5432037.1"
                     /translation="TNAADIFGLQQKGRIAPGKDADFVFIQPNSSYVLTNDDLEYRHK
                     VSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILKHQQ"
     gene            324..1625
                     /gene="ybbY"
                     /locus_tag="ACIYSB_005208"
     CDS             324..1625
                     /gene="ybbY"
                     /locus_tag="ACIYSB_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HGC5432038.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            1647..2792
                     /gene="glxK"
                     /locus_tag="ACIYSB_005209"
                     /pseudo
     CDS             1647..2792
                     /gene="glxK"
                     /locus_tag="ACIYSB_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
     gene            complement(3020..3763)
                     /gene="allE"
                     /locus_tag="ACIYSB_005210"
     CDS             complement(3020..3763)
                     /gene="allE"
                     /locus_tag="ACIYSB_005210"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGC5432039.1"
                     /translation="MANRAIVKHGNFALLTPDGLVKNIIPGFENCDATILSTPKLGAS
                     FVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFALSEGGYLYCPPGSL
                     MTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYEGMDDVILLDFLPKE
                     LGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNNWIPVKKGDYIFMGA
                     YSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            3978..>4724
                     /locus_tag="ACIYSB_005211"
     CDS             3978..>4724
                     /locus_tag="ACIYSB_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HGC5432040.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTVFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGASPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLESYAKEKRTDYESLKSRINKNIIIKIGAMPLDKCELRHWLACF
                     DRVAKQAPVSAGFLLQTCKQALKFCRRRRYAISNVLDDLNVADVGKKPDIGERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGAR"
BASE COUNT         1111 a         1099 c         1260 g         1254 t
ORIGIN      
        1 actaacgcag cagatatttt cggtctgcag caaaaaggcc gtatcgcccc aggaaaagat
       61 gccgacttcg tcttcattca gccgaatagc agctatgttc ttaccaatga cgatctggaa
      121 tatcgccaca aagtcagccc gtatgttggc cgtaccattg gcgcgcgtat cacgaaaacc
      181 atcttacgtg gtgatgtgat ttacgacatt gaacagggct tccctgttgc gccgaaaggt
      241 caatttatcc ttaaacatca gcagtaatct ggcccctgca atgcccgtcc ttgcggcggg
      301 cattctccgg ttaaggtgtg tttatgttca attttgcagt cagccgcgaa agcctgttat
      361 caggatttca gtggtttttc tttatttttt gcaacacggt tgtggttcct cctacgctac
      421 tttctgcttt tcagttgccg caaagtagcc tgcttacgct cacgcaatat gcttttcttg
      481 ctaccgcact ggcctgcttc gctcaggcgt tttgcggtca tcgtcgcgct attatggaag
      541 ggccaggtgg cctgtggtgg ggaaccatcc ttactatcac ccttggtgaa gcatcgcgcg
      601 ggacaccgat caacgatatc gccaccagcc tggcagtggg gattgcactc tccggcgtgc
      661 tgacgatgtt gattggtttt agcggattag gccatcgcct ggcacggtta tttacgccgt
      721 cggtgatggt cttgtttatg ttgatgctgg gcgcgcagct gaccactatc tttttcaaag
      781 gtatgctcgg gctgccgttt ggcatagccg acccgaattt taaaattcag ttaccgccgt
      841 tcgcgctctc ggtggcggtg atgtgcctgg tactggcgat gattatcttc ctgccgcaac
      901 gttttgcccg ttatggcctg ctggtcggca ccataaccgg ctggttgttg tggtactttt
      961 gctttccttc ttcgcactcg ctctccggtg agttgcactg gcagtggttc ccgctcggca
     1021 gtggcggtgc tttgtcgccg ggaattattc tgacggcggt gattacaggt ctggtaaata
     1081 tcagcaatac ctacggtgcg attcggggca cggatgtttt ttatccgcag cagggcgcag
     1141 ggaatacgcg ttatcgtcgt agctttgtgg cgaccggatt tatgacgctg ataaccgtac
     1201 cgctggcggt aattccattt tcaccgtttg tttcatccat tggtttatta acccagactg
     1261 gcgattacac gcggcgttcg tttatttatg gcagcgttat ttgcctgctg gtggcgctgg
     1321 ttcctgcact cacgcgactg ttttgcagta tccctttacc cgtgagtagt gcggtcatgc
     1381 tggtttctta tctgccttta ctcttttccg cgctggtgtt tagccagcaa ataacgttta
     1441 ccgctcgcaa tatttatcga ctcgcattgc cgttatttgt cggcatattt ttaatggcat
     1501 taccgcctgt gtatctgcaa gaccttccat taacgcttcg tcctctgctc agtaacggct
     1561 tattggtcgg gattttactg gctgttctta tggataacct tattccgtgg gaacgcatcg
     1621 aataatttgt tgaaaaagga ttgataatga agattgtcat tgcgccagac tcttttaaag
     1681 agagcttaag tgcagaaaaa tgttgtcagg caattaaagc cgggttttcg accctctttc
     1741 ccgatgcgaa ctatatctgt ttgccgatag cggatggcgg cgaagggacg gtggatgcga
     1801 tggtcgccgc gacgggcggc aacatcgtga cgcttgaagt ctgcgggccg atgggcgaaa
     1861 aagtgaatgc tttttatggc cttaccggcg acgggaaaac ggcggtgatt gagatggcgg
     1921 cagcaagtgg cctgatgctg gtcgcgcctg aaaagcgtaa tccgttgctg gcctccagtt
     1981 ttggtacggg ggagttaatt cgtcatgcgc tggataacga cattcgccat attattctcg
     2041 gcattggcgg cagtgcgacg gttgacggcg gtatgggcat ggcgcaggcg ctcggtgtgc
     2101 gtttccttga tgccgacggt caggcgctgg cggcaaacgg tggtaattta gcgcgcgtgg
     2161 caagcattga gatggatgaa tgcgatccgc gtctggcgaa ttgccatatt gaagtagcat
     2221 gtgacgttga taacccgctg gtaggggcac gcggcgcggc ggcggtgttt ggcccgcaaa
     2281 aaggggcaac gccggagatg gtcgaagaac ttgaacaggg gctgcaaaat tacgcccgtg
     2341 ttttataaca gcaaactgaa attaatgtct gccagatggc gggcggcggc gctgcgggcg
     2401 gtatgggtat tgcggcggcg gtatttctca atgcggatat taaaccgggc attgaaattg
     2461 tgttgaatgc ggtcaatctt gcgcaggcag tgcagggcgc agcactggtg attaccgggg
     2521 aagggcgcat cgactcgcaa acggcaggcg gtaaagcgcc gctgggtgtg gcgtcggtgg
     2581 cgaagcagtt taatgtaccg gtgattggga ttgctggcgt attgggtgat ggcgtggaag
     2641 tggtgcacca gtacggcatt gacgcggtat tcagcatttt gcctcgtctg gcacctttag
     2701 ccgaagtgct cgccagcggt gaaaccaatc tcttcaacag cgcgcgaaat attgcctgcg
     2761 ccattaaaat aggtcaggga attaaaaact aacccttacc tttaaagcgg atgcgattta
     2821 tatcgcataa gagtgcagta ctcatgccgg atgcggcatg agtaccatat ccttcctgaa
     2881 aatcgcgcaa attctatata ttgcagagat catgtaggcc tgataagcga agcgcatcag
     2941 gcaatgttac aaaaaaagcc acggtataaa ccgtggcaaa atccaacata gctaaaaata
     3001 atcaggcgag tggtatgact taaatctcta cgtcgcggtt acaatctttc gagtaaatat
     3061 agctgaacgc ttcaccacgc cctacaccat aaccagcctg taaagaataa gcgcccataa
     3121 agatgtaatc gccttttttc accgggatcc agttattgtc gaggttataa accccctgac
     3181 cggaaagaat ataggcaccg tgttcctgaa cgtgtgtttc gatataaccg tggctggcac
     3241 ctggtgcaaa agagaggata tgcatgttca tatcaaaacc taactctttg ggcagaaaat
     3301 ccagcagaat aacatcgtcc atgccttcat aatgaatgcg ttccagttcg ctggcattgc
     3361 cagaaaccag ccacggtgca tagccttcta ccggaacata gcggcgctta tataaaaaga
     3421 tttggctgtc ttcggcctgg gcgttaacaa acgtcattaa ggagcctggc gggcaataaa
     3481 gatagccacc ttcgcttaag gcaaatgttt tgccttcggc tttggcagtg atatttccag
     3541 agatcacata caggaacgtt tcaatgcctt cgccaccgaa gccctgttgg ttgccaccgt
     3601 tttgatgcag tgtgaccaga taatcaacaa aagaggcacc cagctttggc gtggagagga
     3661 ttgtcgcgtc acaattttca aagcccggaa taatattttt taccagacca tccggggtta
     3721 acagtgcgaa attaccgtgt ttaacaatcg cacggttagc cagtaaatct tcgcggtaac
     3781 cggtgacgtt atttaaatat cccttttatc aaatagtgtt ttttactgtt tcataatgtt
     3841 tcaggttgtg cattatgttt ttgattataa acaaatttgt gatttttctt gttccagctt
     3901 gtttcacgtt gtagcccttt gtgcaacgat cggtgtagtt aatggtgtag ttaattgtga
     3961 tgaccggatt caaaattatg agcagagcac ttaacaagct gagcgataca cagctaagga
     4021 aaatcaacgg cacacccgcc caaaaaacag tctttcttaa tgacggtgga aacttgagcg
     4081 tcaggcattc aaccagcggc cttttaacct ggtatttcac ttacagggcc ggaacgggaa
     4141 ggggggcatc accggaacgc attaagctgg gaaattatcc tgatctgagc ctgaaatcag
     4201 ccagggaaaa agccgcccag tgtcgcgcat ggctggctga ggggaaaaat ccacgtcatg
     4261 agatgaatta caccgtacag gaagcgttaa agcctgtaac ggttggcgat gcgctcacct
     4321 actggcttga gtcgtacgca aaggaaaagc gcacagatta cgaatcactg aaaagccgga
     4381 tcaataaaaa cataatcatc aaaattggtg ctatgccgct ggataaatgc gagctacggc
     4441 actggctggc ctgttttgat cgggtggcaa agcaagcccc tgtttccgcc gggttcctgc
     4501 tacagacatg caaacaggcg cttaagttct gccggaggcg gcgctatgca atcagtaacg
     4561 ttctcgatga tctgaacgtg gcggacgttg ggaaaaaacc ggatataggc gaacgtgtct
     4621 taagcaataa agaactgggc gaattattgc aggcactgga caaaaaaata ttttcccctt
     4681 actacgtcgc gttaatccgc ctcctgattg tcttcggagc cagg
//
LOCUS       DBBGLQ010000209         4660 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.209, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000209 DBBGLQ010000000
VERSION     DBBGLQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.209"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            190..1233
                     /locus_tag="ACIYSB_005212"
                     /pseudo
     CDS             190..1233
                     /locus_tag="ACIYSB_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1238..1285
                     /locus_tag="ACIYSB_005213"
                     /pseudo
     CDS             1238..1285
                     /locus_tag="ACIYSB_005213"
                     /inference="COORDINATES: protein motif:HMM:TIGR01053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1400..3370
                     /locus_tag="ACIYSB_005214"
     CDS             1400..3370
                     /locus_tag="ACIYSB_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGC5432041.1"
                     /translation="MYMNVIRTVICTLIIVPVGLQAATSHSSMAKDTITIVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYVDWVLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHRGVYAGWRAELSASATHVKKGDSYQAGYAIHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            3389..4180
                     /locus_tag="ACIYSB_005215"
     CDS             3389..4180
                     /locus_tag="ACIYSB_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HGC5432042.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNVIAEIGYGHN
                     FPSKGTIPDRRDFFETPRLYNGKETITLKPTSTDYVYKIESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQYPIGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFQDKNLCDETVDVATLSFSRN"
     gene            4425..4595
                     /locus_tag="ACIYSB_005216"
                     /pseudo
     CDS             4425..4595
                     /locus_tag="ACIYSB_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1336 a         1032 c         1122 g         1170 t
ORIGIN      
        1 ttgaataatt tatataactc ttctatatta catcaatgcc gatagaaatc gtaaaaagct
       61 tcgttttcat aaaaatctct ttcaataata tttgtagctg gaccagcccc ttgaaacgcc
      121 agatagtgga tgtatcttaa aataagagac aggcttggat atatgtctga cactggttct
      181 aattttgaga tgactggaac tttgccgggg ctggagcttc gtaaacgtaa aactcctcag
      241 gaaaaaattg gcattattca gcagacaatg gagcctggca tggcggtgtc acatgtggcg
      301 cgtttgcgtt gtattcagcc ctgcctgttg tttaagtgga aaaacaatct ctggaaggaa
      361 gtctcactgc tgttgcttca ggagagggtg tcgttcctgc ctcagaactg cctgccgcta
      421 taaaacaaat aaaagagtta cagctcttgc tgggtaaaaa accatggaag tttagatcct
      481 caaagaagtg gtggagtatg cgcaatcgcg aaaatggata gcgcatgtgc ccttgttacc
      541 ttcggacaag gaatagcccc ggtaagccgt gttctgggcg atactcaggc ggcagtccct
      601 aaatgaagga ctaccgttag tgaatgcaaa gcgcgtttac cggataatga gcgaaaatat
      661 cctgttgtta catgacaaac tatcacgact gcagcgggaa cataaaggcc gaatatcggt
      721 aaggaaagcg accagcgttg atgttctgat ggctttgagt tcggttgcga tgatggcgaa
      781 aaagtccggg ttacgttcgc cctggactgt tgcgatcgtg aagctatcga ctgggcatcc
      841 aatacgggag gttacaataa agcgacagtg caggatgtga tgttaggagc gatagaaaaa
      901 ctctttggcg ataaagtacc ggaacagtcc atccggtggt tgacagataa cggttcagcg
      961 tatagagcgc atgaaacacg gcagttcgcc agagagttgg atctggagcc ctgcacaacg
     1021 gcaatcagca gctctcagag taatggaatg gcagaaaggt tagtgaaaac gatgaaggaa
     1081 gactacattg cgttcatacc gaaaccgaat gtaataacgg cattgcataa ccttgcatag
     1141 gcgttcgaga attacaatga gaaccacccg catagtgcgt taggttatct ctcaccacgg
     1201 gaatatcgat gtcagcggat aacgtcacct tagatgtagc agttgccaaa cagtgactca
     1261 agggcaggct taccgcatac gctgacactt agctgatcga cagaatatta ttaacagttc
     1321 atcactgaac gctacataat cagagttgca taaataaaat gttatgttat aacattttaa
     1381 taccaacata tgttttcgaa tgtatatgaa tgtaatcaga actgtcattt gtacattaat
     1441 tatagtcccg gtgggactac aggcagcgac cagtcattct tctatggcta aagatacaat
     1501 caccattgtc gcgacaggaa atcagaacac ggtatttgaa acgccgtcga tggtcagtgt
     1561 cgtcacgaat gacacaccgt ggagtcagaa tgcggttaca tcggccggca tgctgaaagg
     1621 tgttgccggt ctcagccaga ctggtgcagg acggaccaat gggcagacct ttaatctacg
     1681 cggctatgac aaaagcgggg tacttgttct tgttgacggc gtccgccaac tcagtgacat
     1741 ggcaaaaagc agtggcactt atctggatcc ggctcttgtc aaacgtatcg aagttgtccg
     1801 cgggccaaac tccagtctgt acggcagtgg cgggctggga ggtgtagtgg acttcagaac
     1861 tgccgatgca gcagatttcc ttccccccgg agagacaaac ggtttaagtc tgtggggaaa
     1921 tattgccagt ggtgaccaca gcacaggctc ggggctcacc tggttcggta aaaccggaaa
     1981 aacagatgcg ctcctttctg tcattatgcg taaaagaggt aatatctatc aaagtgatgg
     2041 tgagcgcgca cctaacaagg aaaaacctgc agccctgttt gcgaaaggct ctgtcggtat
     2101 aacagacagt aacaaagcag gtgccagctt gcgtctctac cggaataaca ccactgaacc
     2161 gggcaatccc actcagacac atggtgacag cggcctgcgt gacagaaaaa cagtacaaaa
     2221 tgacgtacag ttctggtacc agtacgctcc tgtggataac agcctcatca atgtaaagtc
     2281 aacgttatat ctcagtgata tcactatcaa gacaaacggt cacaacaaaa cggcagaatg
     2341 gagaaacaac agaacctccg gtgttaatgt tgtcaacagg agtcatactc tgatttttcc
     2401 gggagcccat cagttaagtt atggcgctga atattaccgt cagcagcaga agccagaagg
     2461 ctctgccaca ctatatccgg aaggaaatat tgactttaca tcgttgtatt tccaggatga
     2521 aatgacaatg aaaagctacc ctgttaacat tatcgtcggt tcccgctatg accggtacaa
     2581 gagcttcaat ccccgtgccg gagaactgaa agccgaacgc ctgtccccaa gggcggcgat
     2641 ttcagtctca ccgacagact ggctgatgat gtacggctcc atatcctctg cattccgagc
     2701 gcccacaatg gcagaaatgt acagggatga tgtacatttt taccgcaagg gtaaacccaa
     2761 ttactgggtt cctaacctta atctgaaacc agaaaataac atcacccgtg agattggcgc
     2821 aggtattcaa ctggatggcc tgcttacaga caatgaccgg ctgcagttaa aaggcggata
     2881 tttcggaacg gatgccagaa actatattgc cacacgcgtg gatatgaaac ggatgcgttc
     2941 ttattcttat aatgtatccc gggcccgtat ctggggatgg gatatgcagg gaaattacca
     3001 gtctgattat gttgactggg tgctttctta taaccggacg gaaagtatgg atgccagcag
     3061 cagggaatgg ctgggctccg gcaatcctga cacacttatc agtgacatca gcatacctgt
     3121 tggtcataga ggcgtttatg ccggatggcg tgctgaactt tcagcatcag ccacgcatgt
     3181 gaaaaaaggc gactcctatc aggctggcta tgccatacat tccttttcac tgtcttataa
     3241 gcctgtaagt gttaaaggct ttgaggcgtc agtaactctg gataatgcct tcaacaagct
     3301 tgccatgaat ggcaaaggtg tgccgctttc aggccgaact gtcagtcttt atacccgtta
     3361 tcagtggtaa cctctcagga aataaatcat gatgaaaaat acaggctata tcttagctct
     3421 ttgtctgaca gcatcggggc atgtcctggc ccatgatgtc tggattaccg gcaaacaggc
     3481 agagaacaac gttatcgcag agattggtta tggtcataat ttcccctcaa agggcacgat
     3541 tcctgacaga agggatttct ttgaaactcc ccggctttat aacgggaaag agacaataac
     3601 gctgaagcca acatccacgg attatgtcta taaaattgag tctgcaggca acggtaatag
     3661 ctacgttctg tcaacgaaaa tgaaaccggg atactggtcg agaacctcgt caggatggaa
     3721 gcctgtcagc cgggagggca gaaatgatgt ggcatactgt gagtttgtga ctaaatatgc
     3781 aaaatctttt attcctggtg aacagcagat gccagcacaa ctctatcagt atccaatagg
     3841 ggatgagctt gaaatcattc cgttatccga tatcagtcgt ttcggtgaag atgtgaagct
     3901 gaaagttctg tataaaactt ccccgcttgc cggagccact ctggagcttg actctgtcag
     3961 ttatctgaaa tcatcccgcc atactcatgc ggcagagcat aaacattcgg ctcataaagc
     4021 agaacttacc tttgtaagta atgaggacgg tatcatcact gtaccctccc ttcatgtcgg
     4081 gcaatggcta gctaaagtca aaaataagaa agtatttcag gacaaaaacc tgtgtgatga
     4141 aactgtcgat gtggcaacct taagcttctc ccgaaattaa taatactatt cctgccctgt
     4201 aatttacggg cagggtatat caaccggaaa tagcctactg acatccatgt ataaagccac
     4261 ataattattt tgttgatgta tcttacctaa tacgtaagcg taaagccaat gccgtctgta
     4321 actcctgatc cttgcagact aaatgtaagc gctccttctg tgccgtcttg tggtggttgt
     4381 cctgtctggc aacaatcgtg tccatctatt gatggacacg aatgatgaat tctcagacaa
     4441 caaaagatat caccggcttt cgctcttatt ttccagatgt attgcgacta atattcgaag
     4501 agcaattatc tgttcgggct atcgcactgg agcagttagg cctcagtcat tccaccgttc
     4561 ataatatctt tcaacggttt aatgcctctg gcatgtaagc gtaaaccgac cgttgtattg
     4621 acgcttattt ataggggaga actacgttcc atggcaggag
//
LOCUS       DBBGLQ010000210         4650 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.210, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000210 DBBGLQ010000000
VERSION     DBBGLQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.210"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            213..1187
                     /gene="hemB"
                     /locus_tag="ACIYSB_005217"
     CDS             213..1187
                     /gene="hemB"
                     /locus_tag="ACIYSB_005217"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGC5432043.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1294..2145)
                     /gene="tauD"
                     /locus_tag="ACIYSB_005218"
     CDS             complement(1294..2145)
                     /gene="tauD"
                     /locus_tag="ACIYSB_005218"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGC5432044.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2142..2969)
                     /gene="tauC"
                     /locus_tag="ACIYSB_005219"
     CDS             complement(2142..2969)
                     /gene="tauC"
                     /locus_tag="ACIYSB_005219"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGC5432045.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVATL
                     QLISPLFLPPPQQVLEKLLTIAGPQGFMDATLWQHLAASLTRIMLALFAAVLFGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSVQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2966..3733)
                     /gene="tauB"
                     /locus_tag="ACIYSB_005220"
     CDS             complement(2966..3733)
                     /gene="tauB"
                     /locus_tag="ACIYSB_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGC5432046.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3746..>4650)
                     /gene="tauA"
                     /locus_tag="ACIYSB_005221"
     CDS             complement(3746..>4650)
                     /gene="tauA"
                     /locus_tag="ACIYSB_005221"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGC5432047.1"
                     /translation="QAVNVTVAYQTSAEPAKVAQADNTFAKESGATVDWRKFDSGASI
                     VRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTISKPEDLIG
                     KRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPAIIAAWQRGDIDGAYVWAPAV
                     NALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIAN
                     PDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAIIDTAQF
                     LKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1057 a         1356 c         1196 g         1041 t
ORIGIN      
        1 cgcaaccaaa ctccaaaaaa taataaccaa cagctataaa taaagattaa aaatattttc
       61 agtgaatata aaattattgc tgtttctatt aaaaagatgt cctggcctct cttccattct
      121 cttcttgtca acatcgcgac aactttcgta aaacatccct accctgcttc aggtatacta
      181 tgcccctcga ttccacaaac atcaggcaga ccatgacaga cttaatccaa cgccctcgtc
      241 gcctgcgcaa atctcctgcg ctgcgcgcta tgtttgaaga gacaacactt agccttaacg
      301 acctggtgtt gccgatcttt gttgaagaag aaattgacga ctacaaagcc gttgaagcca
      361 tgccaggcgt gatgcgcatt ccagagaaac atctggcacg cgaaattgaa cgcatcgcca
      421 acgccggtat tcgttccgtg atgacttttg gcatctctca ccataccgat gaaaccggca
      481 gcgatgcctg gcgggaagat ggactggtgg cgcgtatgtc gcgcatctgc aagcagaccg
      541 tgccagaaat gatcgttatg tcagacacct gcttctgtga atacacttct cacggtcact
      601 gcggtgtgct gtgcgagcat ggcgtcgaca acgacgcgac tctggaaaat ttaggcaagc
      661 aagccgtggt tgcagctgct gcaggtgcag acttcatcgc cccttccgcc gcgatggacg
      721 gccaggtaca ggcgattcgt caggcgctgg acgctgcggg atttaaagat acggcgatta
      781 tgtcgtattc gaccaagttc gcctcctcct tttatggccc gttccgtgaa gctgccggaa
      841 gcgcattaaa aggcgaccgc aaaagctatc agatgaaccc aatgaaccgt cgtgaggcga
      901 ttcgtgaatc actgctggat gaagcccagg gcgcagactg cctgatggtt aaacctgctg
      961 gagcgtacct cgacatcgtg cgtgagctgc gtgaacgtac tgaattgccg attggcgcgt
     1021 atcaggtgag cggtgagtat gcgatgatta agttcgccgc gctggcgggt gctatagatg
     1081 aagagaaagt cgtgctcgaa agcttaggtt cgattaagcg tgcgggtgcg gatctgattt
     1141 tcagctactt tgcgctggat ttggctgaga agaagattct gcgttaattt tgtttcctct
     1201 cagaaagggg gagttaacct gttgaaagta cggtgaacgc cccctcaccc taaccctctc
     1261 cccaaggggg cgaggggacc gtccactctc gtattacccc gcccgataaa acggtttatc
     1321 cccaaggatc gtcgcccgat gcattatccg tcgctgtggc aggtaatcgg cattggcata
     1381 gtgctgggtc acgcggttat cccaaatcgc aatatcattt ggttgccagc gccagcgcac
     1441 ctgaaactcc ggtttggtga tatgggcaaa caaaaaactt aacaaggctt cgctctcttt
     1501 ctcgctcaca tcaacaattc gcgtagtaaa gccttcattc acaaacagcg cctgtttacc
     1561 gctcaccgga tgcgttcgca ccaccggatg tagcaacggc gggtttttcg cgaccgcctc
     1621 gcgccagcgt tgatgttcct cctcggtttt gcggtatttg tattccggga acgatttacg
     1681 gaaatcatgc tccgcacgca gcccactcag cagctggcgg aagggaacag agagcgcctc
     1741 ataggccgca ataccgctgg tccagagcgt atcaccgccg gtcgaaggta actctttagc
     1801 tgccagaatc gcccctgcgg gtggcgtttc aataaatgtc acatcggtat gccagttgtc
     1861 gttatctggc ggattatcgt tatgggtatc cagcacgatg atctcgtcaa ccccttcggc
     1921 atgcgggtaa acagggtgaa tatgcaattc gccaaaacgc tgggccagcg cgcgttgctg
     1981 ctgcggcgta atagcttgat cgcgtagaaa caccacctga tggcgcagca ccgcatggta
     2041 aagctgttca aactgattat cgcttaacgg gcgcgtcagg tcggcacccg aaatttgtgc
     2101 gccgatatac ggccccagcg gggtaatgct cagacgttca ctcattgtac ttctccatgc
     2161 cagggcgtca ggcggcgctg taacgcgcgc agacccagtt ctaaaagaaa ggcgataatc
     2221 gcaatcaccg cgatccccgc cagcaccacg tcagttgcga gaaattcacc cgctgactga
     2281 accataaatc ctaaaccgcg cgtcgcggca atcagctccg ccgccaccag cgtagaccag
     2341 cccaccccca gaccaatacg taatccggtg aggatttccg gcagcgcacc gggcaaaatg
     2401 acaaaccaca gcacctgcgc acggctggca cccagcgact gggcggcacg aatgcgaacc
     2461 tgctgcacgc ttttcacccc cgccagcgcc gacatcgcca ccggtgcaaa aatcgctaaa
     2521 tagatcagta agatcttcga ggtttcacca ataccaaacc agatcaccat cagcggcaaa
     2581 taagccagcg gcggcaccgg acgataaagc tcgattatcg gatccagaat gccgcgtacc
     2641 gtagggctaa gtcccatcgc gatcccgacc ggaataccga acaacaccgc tgcaaatagc
     2701 gccagcataa tgcgcgtcag actggctgcc agatgctgcc acagcgtggc gtccataaag
     2761 ccttgcggtc cggcaatggt gagtagtttt tccagtacct gttgcggcgg cggcaaaaat
     2821 agcgggctaa tcagttgcag cgtcgccacc gtccaccata cggtgagtaa aaccgctaac
     2881 gtgccaatgc ttaaggtcac ctgacgcgag agcggccagc gccatttcag ccgccgcgaa
     2941 tgcagttttt cattaatgag cacactcatg agaacgcctc ccgttgctca aatacgcggc
     3001 ttaaaacata ttcgcgcatg gcgatgaatt gtggatcgga cttgatgctg cggctcgact
     3061 ctcccgcaac aaagcggcga gcaaagttga gcggcagccg ctccagcaca cggccagggc
     3121 cggatgaaag cagaaccagt tcagtcgcca taaacaccgc ttcttctata tcgtgggtaa
     3181 tcaacagcac ctgcttgccc gtctcctgcc agagtttcag cagcagggtt tgcatctggt
     3241 cgcgggtgaa ggcgtccagc gcaccaaacg gttcgtcgag taataacagc tggggattcg
     3301 ccgccagcgc acgagcaatc cccacccgct gacgttgacc accggaaagc tgccagatgt
     3361 agcgtttttc tgcgccttcc agccccactt ttttcagcat ctggtgcgcg atttccagtc
     3421 gctgcatttt ctctatacct gccaattgca ggccgaacgc cacgttgtcc tgtacattgc
     3481 gccacggtag tagcccttca ttctgaaaaa ctacgccacg ctctgctccc ggtccctcaa
     3541 tacgcttacc cgccagttga atgctgccat gctgataagg cacaaaaccg gcaatcagat
     3601 tcagcagggt ggttttaccg cagccggacg gccccagcac caccagtagc tcgccgcttt
     3661 ccagcgtcag gttgatatct tccagtgccg gtttgccgcc ataatcggcg taaagatgag
     3721 agatttgcag catctgcgcc tccttttatt gcacgaagcg cgaggtaacg tactggctgt
     3781 aatcattcgc tacagccggg accttgccct gctctttcaa aaactgcgcg gtgtcgatga
     3841 tcgctttgtt caccggtccg gtcagttctg ccgtttgttg ctgcggcgtc agataggtat
     3901 tccccttcac cagccccgga acgtcacctt caggcacgcc gcttaaacgc gccagtttgc
     3961 tgatgttttc cggctgtttc agccacacgt ctgggttagc aatgtacggt tgctgagcat
     4021 cgatggcgct tttagcgaac gctttcacga cctcaggatg tttctcggca aaatctttgc
     4081 gcaccaccca gacgtccagc gttggcgcgc cccactgccc gacctgttca gaatcggtca
     4141 acaccttgcc gtctttttcc agggcgttaa ccgccggtgc ccagacataa gcaccatcaa
     4201 tatctccccg ctgccaggca gcgataatcg cgggcggctg caggttcaca atctccactt
     4261 gcccgggttt aatgccccag tgtttcagtg ccgccagcag gctgtagtgg gtggtggaga
     4321 taaacggtac ggcgatgcgt ttgccaatca gatcttccgg tttgctgata gttttcttta
     4381 ccaccagcgc ttcggagtta cccagttttg acgccagcaa gaagacttca atcggcacct
     4441 gttggctggc tgcaaccgct aacgggctgg aaccgaggtt gccgatttgc acgtcgcctg
     4501 aagccagcgc ccgcacgatg ctggctccgc tgtcaaactt acgccagtcc acggttgctc
     4561 cgctttcttt agcaaaggtg ttgtcggcct gagccacttt cgccggttcg gctgaggttt
     4621 gatacgccac ggtgacgttc accgcctgtg
//
LOCUS       DBBGLQ010000211         4627 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.211, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000211 DBBGLQ010000000
VERSION     DBBGLQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.211"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..371)
                     /locus_tag="ACIYSB_005222"
     CDS             complement(<1..371)
                     /locus_tag="ACIYSB_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432048.1"
                     /translation="MAVFPVDKPTSNYIGVGRRFIISPLPREQAENTPDGVVDLNYSL
                     VANQSLKPFFQSERVFNALGGEDSLVHWVSSNIHDCQAHDKRDCSHQLTTHFYNGSAV
                     RLCWKHDAEYMMKGYSKLDDQ"
     gene            complement(511..1203)
                     /locus_tag="ACIYSB_005223"
     CDS             complement(511..1203)
                     /locus_tag="ACIYSB_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGC5432049.1"
                     /translation="MNQALHEMNLYSRIDGSGYRNIWVVGDLHGCYTLLMNELHRVDF
                     DPTQDLLISVGDLIDRGTENVECLELLQMPWFRSVMGNHERLMIDALSPAGNVNNWLM
                     NGGQWFFMLDTDQEILALALVELVKRLPYIIELNTGHETIVIAHADYPGGEYQFGKDV
                     SLFDVVWSRSRVGDSIDGIGGEITGVDRFIFGHTPVRRPKAYWNQHYIDTGAVFCGNL
                     TLMQVKGGQLKV"
     gene            complement(1200..1511)
                     /locus_tag="ACIYSB_005224"
     CDS             complement(1200..1511)
                     /locus_tag="ACIYSB_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432050.1"
                     /translation="MSIIKEMPVERDEYGCWTHPEYEKFCAGREYISTEEFDAWMKEN
                     NLQWTIRSMDEDDFNLDADGPDIAAWEPERPEGEGWFVGSIHDTEDGPVCIWLREKVA
                     A"
     gene            complement(1508..1732)
                     /locus_tag="ACIYSB_005225"
     CDS             complement(1508..1732)
                     /locus_tag="ACIYSB_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432051.1"
                     /translation="MTEQTMTNRELVDAAIELAGDFYSMMGYEHRPGFKYWESPHPQE
                     QQVFEMACRAFEFIRGSDVMEAVADLEDEE"
     gene            complement(1729..2004)
                     /locus_tag="ACIYSB_005226"
     CDS             complement(1729..2004)
                     /locus_tag="ACIYSB_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGC5432052.1"
                     /translation="MNIDTTITIDTALNTGLALLGWLYIMSSTWRWLGSIFLKQWKKR
                     RKQELRQKALEAFYDAFELGRIEPGTTARIATKGDLMIVMFRQEKTK"
     gene            complement(2001..2249)
                     /locus_tag="ACIYSB_005227"
     CDS             complement(2001..2249)
                     /locus_tag="ACIYSB_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432053.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMVLKAEKQEPPQ"
     gene            complement(2246..2623)
                     /locus_tag="ACIYSB_005228"
     CDS             complement(2246..2623)
                     /locus_tag="ACIYSB_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432054.1"
                     /translation="MKSEGLTPAQLAERNAEYVTEISRLEKERAALVAENELARKAVQ
                     AFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLAEVRAQGVEMMREHPS
                     IKLCSLTHICDELAAQLRKGGKQ"
     gene            complement(2613..2897)
                     /locus_tag="ACIYSB_005229"
     CDS             complement(2613..2897)
                     /locus_tag="ACIYSB_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGC5432055.1"
                     /translation="MILRQCAGTMTVESIGRLIGRTGDAVRTKARELGIRMILKGDFH
                     QSAKYRQSDIELARQLHQCGVPRREIAEKLEMPLGMINQYVYFERRVHEV"
     gene            complement(2927..3550)
                     /locus_tag="ACIYSB_005230"
     CDS             complement(2927..3550)
                     /locus_tag="ACIYSB_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGC5432056.1"
                     /translation="MVKRVLKIYIAGPMTGYPDYNRAAFDAKASELMAEGHIVLNPAV
                     LPGGLCQSEYMDICLAMVRSADAIYLLKGWDVSAGARAEHALAEKLGLTVIYEAPSDT
                     DCRIAAHLYRELVDALRDVAIECHGTDQLRARLSNTLSSYLSLAEGYNLRQRRMVKLI
                     TRLSQSLANAEPTNPLPNDAMNYLKSCGVVSEDAVRFVEFMSKRMSA"
     gene            complement(3550..3705)
                     /locus_tag="ACIYSB_005231"
     CDS             complement(3550..3705)
                     /locus_tag="ACIYSB_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432057.1"
                     /translation="MMLIALSAIHQPSVNEIGLFYVLFFGALALLELGIELFAVLMFC
                     VTMLGKF"
     gene            3824..4402
                     /locus_tag="ACIYSB_005232"
     CDS             3824..4402
                     /locus_tag="ACIYSB_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family recombination enhancement nuclease"
                     /protein_id="HGC5432058.1"
                     /translation="MQKQSYWEKQRQKAMQKLADPAWREEQRAKRLQQALRQQQRAKE
                     KASSPEYRQKKIEKAKQYEQRRKEKAVSAPSKKTRTSRGLKGRSLTADERRIQTAIGT
                     LPCIACHIHGQHSPVVSLHHIFGRTAENAHKYVLPLCKWHHQYAAPAEVREQYPWLVP
                     VHADGKIGGKADFMRHNADEMALYQMAIELIN"
BASE COUNT         1234 a         1250 c          986 g         1157 t
ORIGIN      
        1 aactggtcgt ccagcttgct gtaccccttc atcatgtatt ccgcatcatg cttccagcac
       61 aggcgaacgg cagagccgtt atagaaatga gtggttagct ggtggctgca atcgcgctta
      121 tcgtgcgcct ggcaatcgtg gatattggaa cttacccaat gaacaagcga atcttcaccg
      181 cccaacgcgt taaatacgcg ctcgctttga aaaaacggtt tcagagactg gttggcaacc
      241 agcgaataat tcagatccac tacgccatct ggcgtgtttt cagcctgttc acgcggaagt
      301 ggggagataa tgaaacggcg acctacgcca atgtagttac tggtgggctt gtctaccgga
      361 aagacagcca caccagctcc gtttacgaaa tgaggtgtga ttattgcact cataatgtat
      421 accgactctg ttttgttccg agcggtaaaa taatatgtta gaaaactaaa atcaatgttc
      481 taacacattt tgagtgcgta aatgatttga tcagacttta agctgtcctc ccttcacctg
      541 cattagcgtc agatttccgc aaaaaaccgc gcccgtgtct atgtagtgct gattccagta
      601 tgcttttggt cttcgtaccg gtgtgtgccc aaagataaag cgatctacgc ctgtgatttc
      661 tccaccaatg ccatctatcg aatcaccgac gcgactgcgc gaccagacaa catcaaaaag
      721 cgacacatcc ttaccgaact ggtactctcc acctggatag tcggcatggg ctataacgat
      781 agtttcatgc ccggtgttca gctcaatgat atagggcaga cgctttacca gctccaccag
      841 cgccaaggct aatatttcct gatcagtgtc cagcatgaag aaccattgac caccattcat
      901 tagccagtta ttcacgttgc ctgcgggact taacgcatcg atcattaatc gctcgtggtt
      961 tcccataacc gaccgaaacc agggcatctg caatagctcc aggcattcaa cattttcggt
     1021 gccgcgatcg ataaggtcgc caaccgatat cagtaaatcc tgtgtcgggt caaaatccac
     1081 acgatggagt tcattcataa gtagggtgta gcaaccgtgc aaatcaccaa caacccatat
     1141 gtttctgtag cctgaaccat caatacggct atataaattc atttcatgca gcgcctggtt
     1201 catgcggcaa ccttctcccg cagccagata caaaccgggc catcttcagt gtcatgaata
     1261 gagccaacaa accatccttc gccctctggt cgctctggtt cccaggcggc aatatcggga
     1321 ccatctgcgt ccagattaaa atcatcttca tccatactgc gaatagtcca ctgaagatta
     1381 ttttccttca tccaggcgtc aaattcctca gtggatatat attcacgacc tgcgcaaaac
     1441 ttttcatact ccggatgtgt ccagcagcca tattcatcac gttctaccgg catttcttta
     1501 atgatgctca ctcttcatcc tccaagtcgg caacggcctc catcacatca gaaccgcgaa
     1561 taaactcaaa agcacggcaa gccatttcaa acacctgttg ttcttgcgga tgcggtgact
     1621 cccaatactt aaaaccaggt cgatgctcgt aacccatcat ggaataaaaa tcgccagcaa
     1681 gttcaatcgc ggcatcaaca agttcgcgat ttgtcatcgt ctgttctgtc attttgtttt
     1741 ctcctgtctg aacatcacta tcatcaggtc gccttttgtc gctatcctgg ctgttgtgcc
     1801 tggttcaatg cggccaagtt caaatgcgtc atagaacgct tctaatgcct tctggcgtaa
     1861 ttcctgtttg cgccgttttt tccactgttt caggaaaatg gaacccagcc atcgccatgt
     1921 actggacatg atgtaaagcc aaccgagaag tgccagaccg gtatttaggg ccgtatctat
     1981 cgttatcgta gtgtcgatat tcactgtggt ggctcctgct tttctgcctt caataccatg
     2041 cgagaaccat catccagctc ccaattaatt tcaccgcctt ctgccatgac aagatgccaa
     2101 acgagttgtg cggcctcgtt ggttacatcg cgaccgcgat cattgccgac acgacgtttt
     2161 gttccatccc ctaagtcacg catttttgcc aacacgatgg tttttgataa cggtgaaaaa
     2221 ccaagctgta gtcgtgctga attgctcact gtttccctcc cttgcgaagc tgggcggcta
     2281 actcgtcaca tatgtgcgtc aaggagcaaa gtttgattga tggatgttcg cgcatcatct
     2341 caaccccctg cgcccgtact tcagccagga aagcgtcggt ggctggcata tttcctgttg
     2401 ccttcatggc ctccaaaata accagaacgc catctctccc aacctcctca cagataacct
     2461 cagtgttgtc gccaacaaca tcgcagaatg cctgaactgc tttacgagcc agctcattct
     2521 ccgccaccag cgccgcgcgc tctttctcaa gtcgggaaat ttccgttaca tactcagcgt
     2581 tacgctctgc cagttgtgcg ggcgttaaac cttcagactt catgcactct cctttcgaaa
     2641 taaacgtact gattaatcat gcccaggggc atttcgagtt tttccgcgat ctcacggcgg
     2701 ggaacgccgc attgatgaag ttgccgcgcc agctctatat cgctctgccg atatttggct
     2761 gactggtgaa agtcaccttt cagaatcatt ctgatcccca gttcccgcgc tttagtcctg
     2821 acagcatctc cggtacgccc gatcagcctc cctatactct cgactgtcat agttccggca
     2881 cactggcgga gtatcatgat ttctgccctt ctccatcccc gccatctcac gctgacatcc
     2941 tcttagacat aaactcaaca aaacgaactg catcttcaga aacaacgccg catgacttca
     3001 gataattcat tgcgtcatta ggtagcggat tagttggttc ggcatttgcc agggattgag
     3061 ataaacgcgt gattaatttg accattctcc gctgccggag gttgtatcct tcagctaaag
     3121 acaggtaact ggaaagggta ttgcttaagc gggcgcgaag ctgatccgtg ccgtgacatt
     3181 caatagcaac atcacgtaac gcatcgacca gttcacggta aagatgcgct gcaattctgc
     3241 aatctgtgtc agatggtgct tcataaatta ccgtcaaccc cagtttttca gccagagcgt
     3301 gttctgcacg tgctcctgcg gacacatccc atcccttaag cagatagatc gcatcagcag
     3361 aacgcaccat tgccaggcaa atatccatgt attcactctg acaaaggcca ccaggtaaca
     3421 cagctggatt cagaacgata tgcccttcag ccatcagctc gctcgctttc gcatcaaacg
     3481 ccgcacggtt gtaatctgga taaccagtca tcggccccgc gatgtaaatt ttcaaaacac
     3541 gtttcaccat caaaatttcc ccagcatcgt gacgcaaaac atcagcacgg caaaaagctc
     3601 aataccaagt tcaagtagcg ccaacgcccc gaaaaacagc acatagaaca gaccgatctc
     3661 attcacagat ggctggtgga tcgcacttaa agctataagc atcatgtcct ccaaacagtt
     3721 atcagcgaag atattaatgt tttatgttag aaaatcaatt ttgattatct aacgatattg
     3781 atcaatcata aaaaaaatcg ctgataagta agaggcagcc atcatgcaga aacagagtta
     3841 ttgggagaaa cagagacaaa aagccatgca aaaattggct gacccagcct ggcgagagga
     3901 acaaagggca aagcgccttc aacaagcttt acgccagcag cagcgagcga aagaaaaagc
     3961 ctcatcgcct gaatatcggc aaaagaaaat tgaaaaagca aagcaatatg aacagcggag
     4021 aaaagagaag gctgtatcag ctccgtccaa aaaaacacgc acgtcacgcg gcctgaaagg
     4081 aagatcactc acagccgatg aacgccggat acagaccgct atcggtactc tcccctgcat
     4141 tgcctgccat attcacgggc aacatagccc agtggtatcc ctgcaccata tcttcgggag
     4201 aacggcagag aacgcgcata aatatgttct ccctttgtgt aaatggcacc accagtacgc
     4261 agcgccagcc gaggtccgag aacaatatcc ctggctggtc cctgttcatg ctgacggaaa
     4321 aataggcgga aaagcagatt tcatgcggca caatgccgat gaaatggcgt tgtatcagat
     4381 ggcgattgaa ttgataaatt agttttctaa cattttaagt tgaattgaaa aatgctccga
     4441 tgtacattca ctaccgattg gcacaccggt caactttttg agacaaacta tttagttttt
     4501 cgtatgattg ccgccgctat accaatggcg gtgcaatata ggtggctgaa aagcccccgt
     4561 tgactcacgg tgttccagcc tttattgcgc cgccaccaga ccgtggaaca gtcgatggcg
     4621 gctccga
//
LOCUS       DBBGLQ010000212         4594 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.212, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000212 DBBGLQ010000000
VERSION     DBBGLQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.212"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..619)
                     /locus_tag="ACIYSB_005233"
     CDS             complement(<1..619)
                     /locus_tag="ACIYSB_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGC5432059.1"
                     /translation="MSLAERFKARRTELGMTQTEVAAIAGVSQQSIETIESGKTRKPR
                     NIIELSKALKCSPDWLLNGRNIMPLAEISTRRIPILSYVQAGALTEAKDATDMDGNME
                     YVLADSDVPESCFALRIDGDSMQPEFKEGDIVIIDPDLCPMPGEFVVAKNNGYEATFK
                     KYRPLGIGVDDFELVPLNPDYPVLRSADVPLQIVGVMIEHRIYRRK"
     gene            736..945
                     /locus_tag="ACIYSB_005234"
     CDS             736..945
                     /locus_tag="ACIYSB_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5432060.1"
                     /translation="MTLATRVKNQRKALGLTQVMLAERTGISQQAINRIESGLISRPR
                     YLLEIAVALNCDPNWLLHGTPADKT"
     gene            949..3093
                     /locus_tag="ACIYSB_005235"
     CDS             949..3093
                     /locus_tag="ACIYSB_005235"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGC5432061.1"
                     /translation="MAQINLSYGRELVDNLPVPCTDADFNRTFKRVKKWARAIGATKQ
                     DTKSQLNAKKARLPYIWHAMKDVTKGRNAANAGNRDVLFMDMDGCNAPAWGALRDALA
                     CFSCFTYSTPSHLHPTANGEHRWRICLELSRQVTPEEYKRLGLAVENWLMDCISLLDD
                     YTVKWDHSVYNPDHMVFAPDEKAIFERFTGEPVDVDILLASAPEVTIPEKASSTDRDD
                     LSRAVELNGISQETFDDLREVMWHPAVLADAEPGCGKYHSWAAMGNRLAWFKGTDWED
                     AARQLWVDWSIAGGGDGISAEHKWDSNQLTAERTGYQAIFAHAQKAGVANPATDRMRG
                     FVAGANDFDELPPEKPELPKSRPHTGGYGKPGKFIIEGMVPHGVTMLYGASSSFKSYG
                     VISMLCRVATKTHRWGGRNLKGGAVLYVAAEGGSSVMPRVGAWADKYNDGKPLDLFYT
                     LPLAVDLSVPANVTKMVNEIKRIAYHTGEPVRLVAVDTLSQSMMQGEENSASDVAKFM
                     AGATRVFTETGAAVIIVHHSGKDSSKGMRGSSAAFANADAVIRVERIGDAVNLINEKQ
                     RTGPVQPTRGYMVPTVQLPDDVIAANETYDDEYTSTEGEVYDPVRLTTERVFEDAPMA
                     EITPLLVDKGDGNTKGSSDESWVWEKLEASGGSINRSALRELWKDETDKSTGQLRAVL
                     GRMKEKYYIVERDGVIRDPLCNGVPPGGDEIA"
     gene            3080..3403
                     /locus_tag="ACIYSB_005236"
     CDS             3080..3403
                     /locus_tag="ACIYSB_005236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432062.1"
                     /translation="MKLLNFTNLQKATNLHKNAKIKYMQFICINSQRNKNFKQQAQHD
                     AQQRNKKCCAADFKGFFNRNKSNTTASVVAQHNTPLPPLGGRGLLRPSRCGFYSFKCI
                     CNAGE"
     gene            3417..4115
                     /locus_tag="ACIYSB_005237"
     CDS             3417..4115
                     /locus_tag="ACIYSB_005237"
                     /inference="COORDINATES: protein motif:HMM:NF017832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HGC5432063.1"
                     /translation="MLTAINNEKIVIARDAQKPGIFACPECGEVVTLRKGSVKVHHFA
                     HRSTTSCGYGKGESELHMDLKMQVYDALNAHREVEYVAMEVAVGDVRPDVLAIIRGQK
                     VAFEIQVSSLSIEDLYRRTVAYAAQDIAMIWILHWPEGTALDRLYHPASWERWLHTCG
                     MATLYFWAGDGWVRPVRLGNRQHGLQRAPRAHLVDDFEPKRTGGWIKGDFLIPARFIW
                     NRVRRQKRRGQSYA"
     gene            4108..4542
                     /locus_tag="ACIYSB_005238"
     CDS             4108..4542
                     /locus_tag="ACIYSB_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGC5432064.1"
                     /translation="MRNIQQVLERWGGWAANSNTTVSWAPIAAGFKGLVVSSLPGRLS
                     CCDDDGLIIDACVCRLQQVRKPEELDVIMLYYVYGLSKREIGRRRRCSEGFIRQQLQV
                     AEGFIEGCLCMLGVSLQMDPEVEIQRDEKSISALRKNCATLV"
BASE COUNT         1109 a         1119 c         1290 g         1076 t
ORIGIN      
        1 ttttgcggcg gtaaatacga tgttcaatca tgacccctac gatctgtaag gggacatctg
       61 cacttcgtag cacgggatag tcgggattca gggggaccag ttcaaaatca tcaacgccga
      121 tacccaacgg gcggtatttt ttgaaagtgg cctcataccc gttgttcttt gcaacgacga
      181 attcgccagg catggggcat aaatccgggt caattattac aatatcgcct tccttaaact
      241 ccggttgcat actgtcacca tctatccgca gggcgaagca ggactcaggt acgtcggaat
      301 ccgcgagaac gtattccata tttccatcca tgtccgtagc gtcttttgct tccgttagcg
      361 caccggcctg tacgtaactg aggatcggga tgcggcgggt acttatttcc gccaggggca
      421 ttatattcct gccatttaaa agccagtcgg gactgcattt cagtgccttt gaaagctcta
      481 tgatgttgcg gggcttccgg gtctttccgc tttcaatagt ctcaatggat tgctggctga
      541 cccccgcgat tgctgcaact tctgtttgtg tcatcccgag ttctgtacgg cgtgctttaa
      601 agcgttctgc aagtgacatg gtgtaccccc tgattcctga ttttttgctc tcccttatta
      661 atacaatttt tgttgtagtt gacaaacatc atttattgtc gctaaatacc acataaattg
      721 taggaggcaa taatcatgac acttgctacc cgagtaaaaa accagcgtaa ggctcttggc
      781 ctgacgcagg ttatgctggc agaacgcaca gggattagcc agcaggccat taaccgcatt
      841 gaaagcggcc ttatatcccg tccccgttat ctgctggaaa tagctgttgc cctgaactgc
      901 gaccctaact ggctgttgca tggtactccc gcggataaga cgtaacttat ggcccagatc
      961 aatctctcat acggccggga actggtagac aaccttcctg tgccctgtac tgatgccgat
     1021 tttaaccgga catttaagcg tgttaaaaag tgggctcgtg ctatcggcgc aaccaaacag
     1081 gatacaaaaa gccagcttaa cgccaaaaaa gcgcgtctgc cctacatctg gcacgcaatg
     1141 aaggacgtca ccaaaggtcg taacgctgct aatgcgggca accgtgatgt gctcttcatg
     1201 gatatggacg gctgtaacgc gcctgcatgg ggcgctttac gcgatgcgct ggcctgtttc
     1261 tcatgtttca cctattcaac gccaagtcat ctgcacccca ctgcaaacgg ggaacatcgc
     1321 tggcgtatct gcctggagct gagtcgccag gtcacgcccg aagagtacaa gcgtcttggc
     1381 cttgcggttg aaaactggct gatggattgt atcagcctgc tggacgatta caccgtcaag
     1441 tgggaccaca gcgtttacaa cccggaccat atggtttttg cccctgatga aaaggccatc
     1501 tttgaacgtt ttaccgggga acctgttgac gttgatatcc tgttggccag cgcaccggaa
     1561 gttaccatcc ctgaaaaagc atcttccaca gaccgcgatg atttgtcacg cgctgtcgaa
     1621 ctgaacggca tcagtcagga aacttttgac gacctgcgcg aagtgatgtg gcatccggct
     1681 gtactggcgg acgctgagcc gggttgcggt aaataccata gctgggcggc gatgggaaat
     1741 cgtctggcat ggtttaaggg aaccgactgg gaagacgcag cccgtcagct ttgggtcgac
     1801 tggtcgatag cgggtggtgg cgatggcatc agcgctgaac ataaatggga cagtaaccag
     1861 cttaccgccg aacgaaccgg ttaccaggcc attttcgccc atgcgcagaa agcaggggtc
     1921 gctaaccccg cgaccgaccg tatgcgcggt tttgtcgccg gtgcgaatga cttcgacgag
     1981 ttgccaccgg aaaaaccgga gctgccaaag tccagaccgc acacaggcgg ctacggtaag
     2041 ccgggaaagt tcatcattga agggatggtg ccgcacgggg tgaccatgct ctatggagct
     2101 tcgtcatcgt tcaaatccta tggcgttatc tccatgctct gccgtgtggc gacgaaaact
     2161 catcgctggg gcgggcgtaa cctcaaaggt ggcgctgttc tgtatgttgc cgctgagggt
     2221 ggcagctccg ttatgccgcg tgtcggcgca tgggcggata agtacaatga cggtaagcca
     2281 ctggatttgt tctacaccct gccactggcg gttgatctct ctgtccccgc aaacgtcaca
     2341 aagatggtaa acgagattaa gcgcatagcg taccacaccg gcgaacctgt ccggcttgtg
     2401 gccgtcgaca cgctgtcgca gtccatgatg cagggcgagg aaaacagcgc cagtgatgtg
     2461 gcaaaattca tggcgggcgc aaccagggtg ttcaccgaaa cgggcgcagc ggtgattatc
     2521 gttcaccaca gcggcaaaga cagcagtaag gggatgcgtg gctcttctgc tgcctttgct
     2581 aacgccgatg cggtcattcg cgtggaacgc atcggcgacg cggtgaacct gattaacgag
     2641 aaacagcgta ccggtccggt acagccgacc cgcggttaca tggtaccgac agtgcagctt
     2701 cctgacgacg ttatcgcggc taacgaaacg tacgatgatg agtataccag cactgaaggg
     2761 gaagtatatg accctgtaag gcttaccacc gagcgcgtgt ttgaagatgc accaatggcg
     2821 gagatcaccc cactgctggt ggataagggc gacgggaata ccaaaggcag cagcgatgaa
     2881 tcctgggtat gggaaaagct ggaggcctcc ggcggcagca tcaaccgttc tgcgcttcgc
     2941 gagttatgga aggatgaaac cgataaaagt accggtcagt tacgtgcggt tctggggcgc
     3001 atgaaagaga agtattacat cgttgagcgc gacggcgtaa tcagggatcc gctatgcaat
     3061 ggcgtaccac ctggagggga tgaaattgct taattttacc aatttgcaga aagctacaaa
     3121 tttgcataaa aatgcaaaaa taaaatatat gcaatttatt tgcataaata gtcagcgcaa
     3181 caaaaatttt aagcagcaag cgcaacacga tgcgcaacag cgcaacaaaa aatgttgcgc
     3241 cgctgatttt aaaggctttt ttaaccgcaa caagagcaac acgacggcta gcgtagtggc
     3301 gcaacacaat acccctctcc cccctttagg ggggaggggt ttgttgcgcc cgagccgttg
     3361 cggtttttat tcatttaaat gcatatgcaa tgctggtgaa tgataagggg gtgcagatgc
     3421 tgacagcaat caacaacgaa aaaattgtca tcgcaaggga cgcgcagaaa ccgggaattt
     3481 tcgcctgccc ggaatgtggt gaagttgtca ccctgcggaa aggttcggtg aaagttcacc
     3541 atttcgccca tcgcagcacc acatcctgcg gttacgggaa aggcgaatct gagttgcaca
     3601 tggatttgaa aatgcaggtc tatgacgcgt tgaacgcgca tcgtgaggtg gaatatgttg
     3661 cgatggaggt cgcagtcggt gatgttcgtc ccgatgtgct ggcgattatt cggggtcaga
     3721 aggttgcttt tgagattcag gttagttcgc tgagtattga agacctctat cgccgcacgg
     3781 ttgcgtacgc agctcaggac attgcgatga tctggatcct gcactggcct gaaggtactg
     3841 cactggaccg gctttatcac cctgcatcat gggagcgatg gttacatact tgcggaatgg
     3901 caactctgta cttctgggcg ggcgatggct gggtcaggcc agtgcgtctg ggaaaccgac
     3961 aacatggatt acaaagagca cctcgtgccc atctggttga tgatttcgaa ccaaagcgaa
     4021 caggcgggtg gattaaaggt gattttctga tacctgctcg ctttatctgg aatcgtgtgc
     4081 gacggcaaaa acgtagaggt cagagctatg cgtaatattc aacaggtttt agagcgctgg
     4141 ggtggctggg ctgccaatag caacaccaca gtgagctggg ctccaatagc ggcgggattt
     4201 aaagggctgg tagtcagcag cctgcctggc aggctgagtt gctgcgacga cgatggcctg
     4261 attatcgatg cttgcgtctg tcggctgcag caggtccgta agcctgagga actggacgtc
     4321 attatgctgt actacgtcta cgggttaagc aaacgtgaga taggacggcg gcgtcgatgt
     4381 tctgaaggat ttatccgcca gcagttgcag gtagctgagg gattcattga ggggtgtctc
     4441 tgcatgctgg gtgtgagcct tcagatggat cctgaggtcg aaattcaaag ggatgaaaaa
     4501 agtattagtg cgctacgcaa aaactgcgct acgctggtat gagttgaatt tctgacctca
     4561 acgaagagct ccaaattatt gggggttttt tgta
//
LOCUS       DBBGLQ010000213         4526 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.213, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000213 DBBGLQ010000000
VERSION     DBBGLQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.213"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..510)
                     /locus_tag="ACIYSB_005239"
     CDS             complement(<1..510)
                     /locus_tag="ACIYSB_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432065.1"
                     /translation="MSDSEFFSFIFTHWIGYMLLTFLCAFLCYKAIRKTTYGIIDPAH
                     YFFAFGSGTAYSVVIILYLYGYIDDYLAIMVLLNFIILVITWRFSFSLNIKPIAGLRN
                     ALFREVDSNYKFSFKIIFVMYVVLTAFYLLNVSFSSFGESRFEANKGLGVIVRILDVV
                     RLLLSAMLAI"
     gene            complement(925..2076)
                     /gene="intS"
                     /locus_tag="ACIYSB_005240"
     CDS             complement(925..2076)
                     /gene="intS"
                     /locus_tag="ACIYSB_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGC5432066.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIQAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWNNVDFENRIITINASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDDK"
     gene            complement(2238..2312)
                     /locus_tag="ACIYSB_005241"
     tRNA            complement(2238..2312)
                     /locus_tag="ACIYSB_005241"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2277..2279),aa:Arg,seq:cct)
     gene            complement(2388..3320)
                     /gene="yfdC"
                     /locus_tag="ACIYSB_005242"
     CDS             complement(2388..3320)
                     /gene="yfdC"
                     /locus_tag="ACIYSB_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGC5432067.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFQVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLIRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNYKNPA"
     gene            3614..4369
                     /gene="mlaA"
                     /locus_tag="ACIYSB_005243"
     CDS             3614..4369
                     /gene="mlaA"
                     /locus_tag="ACIYSB_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGC5432068.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1205 a         1039 c          995 g         1287 t
ORIGIN      
        1 tatagccagc atagctgaca ataacaatcg cacaacatct agtattctta caattactcc
       61 aagaccttta tttgcctcaa accttgattc accaaacgaa gagaatgaaa catttaataa
      121 atagaacgct gttaatacaa catacataac aaaaataatc ttaaatgaaa atttatagtt
      181 gctgtctacc tctctgaaaa gagcgtttct aagaccagct attggcttta tgtttagact
      241 aaatgaaaat ctccatgtta taacaaggat aataaagtta agtagtacca ttatggctag
      301 gtaatcgtct atgtacccgt aaagatatag aattatgaca actgaatatg cagttccaga
      361 accgaaggcg aaaaagtagt gcgctggatc tattattcca taggttgttt ttcttattgc
      421 tttgtagcat aagaacgcgc aaagaaaggt taatagcatg taaccaatcc agtgcgtgaa
      481 tataaaactg aaaaattcac tatcgctcat ttttatttcc gaatagtttt aaaaaaacat
      541 aaacatacga aattattaat acagcaatca cgaatgcatg tggctaactt acaatttgca
      601 tcatactttg tatttatgcc attcatgtta atatcatttt atttccttga caaatcaaca
      661 ttgttacata agaaagtttc ttatgcaaag ttaaaaccag cttgaaaata ttcgtttgct
      721 aacgaaatca actcaatata gtcattgata gttagcacgt ctatctcaaa tgtgttaaga
      781 gtgattttga cataagagga ttctgtgcag agaatttata ccataaacaa aaacttgtca
      841 tggcgggaaa agatggatgc agtatacata atcattagat tacaatgttc tgttccatta
      901 gttatcattt aagtgttagc tatttcattt gtcatcaagc cagtccgccc accactgcat
      961 catctccctg cgcttatcga gatactgcgc atggttgtaa ataccgcgca cagatccgcc
     1021 gttggcatgt gccagttgca cttcaatagc atcagcaggc cattcgtgct cgttcataat
     1081 cgtgctgaat tcatgcctga atccgtgacc gctttccaga ccctcatagc cgatttgttt
     1141 gatcacaagc aataccgcgt tctcgcagat tggctttttc ttatcgttgc ggccggcaaa
     1201 aacaaactct gatactggtt tggtgataga gcttagcgta gtgagaagtt caaccacctg
     1261 gtctgacata ggaaccacat gaattttgcg tcccttcatc acactggcgt tgatagtgat
     1321 aatcctgttt tcaaaatcga cgttattcca tagcatagaa cgaagctctt tcgttcttag
     1381 ggctgtatag cgtaaaacct tggtggcaat gagcgatacg atacttcctg aaaatgttgc
     1441 cagtgcttta ttgaatgcct ggatctggtc tgcaggaaga aacgggaagt tcttcttgcg
     1501 gtatcccttc atggcgtcag caaggtcagg tgccgggtta tatttagccc tgccggtgac
     1561 aatagcgtaa cggaaaacct cgccgcatct tctgcgggct ttgttggctc gctccattgc
     1621 accgcgatct tcaaatctgc ggattacttc caacagttgc atcggctcaa tatcctgaat
     1681 ctcaagaccg ccgatgatgg gcaaaatgtc gtcatcaaac attttggcaa gttcagtcgc
     1741 atagcctact gaccagactt gcttcttgtg ctcgtaccat tccttgtaaa tcgcactaaa
     1801 ggaattgttg ttagacgaag cctttttcgc ctttaccgga tcgatgccag ccgagatgtc
     1861 tttcctcgca gtccatgcct tatcccttgc ctcctgcaaa gtcataagcg gatattttcc
     1921 gacggtcagg attttctcct taccgtcaat cttgtagcga agctgccata cctttttccc
     1981 tgacacaggg acataaaggt acaggccatt accatcgaga aggcggtatg gtttttcttt
     2041 cggctttgct gcttcaatct gcttaacggt gagcatgggt aaaaatccgg tgggtaaaat
     2101 tattttatcc actttttacc cgtcatggag tgcggctgtc aacgatctga cgcgaaccat
     2161 gacgaactgc gaatctacgg aaggcttgat attcagggga ttttgcggac tggtacggat
     2221 gggagcgaac tgataaatgg tgtcccctgc aggaatcgaa cctgcaatta gcccttagga
     2281 ggggctcgtt atatccattt aactaagagg acaatgcggc atgagtatac ccgctaatgg
     2341 agtgcggggt aagtacgctg ccgctcgatt gcttaaaccc tcgccattta tgccgggttt
     2401 ttataatttt tcttaatgtt ttccgcacgt tctgcttttt ggcgtgcttc tgctttacgc
     2461 ttattgctca tgtcgttacg aatctgtgca tgactcatta acgcgaagat aaaggtgccg
     2521 ccgcagatgt tccccgctaa agtaggtagt gcgaatggcc agatgaaatc gctccagtgc
     2581 agcgtaccgt taaacaccag atagaggatt tcaacagaac cgaccacgat atgggtggtg
     2641 tcacccaggg caataagcca ggtcatcaat ataatcacca caatctttgc cgcacccgct
     2701 gcaggaaaca tccaaaccat agtggcgatc agccagccgg aaatgatcgc gttggcaaac
     2761 atctcgctgg gggtgttctt catcacatcc atgccgattt tgacaaatgc atcgcgagtt
     2821 tcttcattga agataggcat atattcaaat gcccacgccg caatacctgt cccgagaata
     2881 ttacccagca gcacgacgcc ccataaccgt ataagtaagc cgacgttgct cattgtcggt
     2941 ttttgcatga cgggtagtac cgcagtcacg gtattttcgg taaataattg ctggcgggcc
     3001 ataatgacga taataaaacc aaaggtataa ccgagattct ccagcaagaa gctgcccggc
     3061 acaccttcca gttcgacttg aaatatccct tttgccagta acgaagcgcc catcgacaga
     3121 cccgccgcaa tggctgacca cagtagcgcc attgcgtcgc gttccagctc tttttcacca
     3181 tcctggcgga tatgctcatg aattgccatc gcccgggagg ggagtcggtc ttcatctatt
     3241 tctatttttt tgccgcgctc tttttcttcg ctctcaactt caatttcgtc gctgtgttga
     3301 tcaattttgt cgttgtccat ggtctcttct cttgaattag cacgtatagc taaagcgtag
     3361 cggctttttt gctcgcaact gacgggagtt actctgaaaa tgtagaaaag gctgcgtttg
     3421 cctttttctg tttctataga atcaagtagc ctacagggcg gcgattacca ggctatgatc
     3481 aaatcagcaa atcagggcgt ctggacatca gttgacgtgc tgttacaatc gcccacacct
     3541 aaacaggcgg atacggtatc gttccgtcat ggatggcaaa ctgcataagc cataaaaaaa
     3601 cagggagaca tttatgaagc ttcgcctgtc ggcgcttgct ctgggaacta cgcttctggt
     3661 ggggtgtgcg agttccggta cagatcagca agggcgttct gacccgttag aagggttcaa
     3721 ccgcaccatg tacaacttca acttcaatgt attagacccg tatattgttc gaccggtcgc
     3781 tgtcgcctgg cgtgattatg ttccgcaacc ggcgcgtaac ggtttgagca actttactgg
     3841 caaccttgaa gaacctgcgg tgatggttaa ctacttcttg cagggcgacc cttatcaggg
     3901 gatggtccac tttacccgct ttttcctgaa caccattttg gggatgggcg gttttattga
     3961 tgttgcaggg atggcgaacc cgaaactgca acggactgaa cctcaccgct tcggtagtac
     4021 gcttggtcat tatggcgtgg gttatgggcc ttacgttcag ttaccgttct acggtagctt
     4081 cacgctgcgt gatgacggtg gtgatatggc ggatggtctt tacccggttc tttcctggct
     4141 gacctggccg atgtctgtgg gtaaatggac gcttgaaggg atcgaaaccc gcgctcagct
     4201 gctggattcc gatggtctgc tgcgtcagtc gtccgatcct tatattatgg tgcgcgaagc
     4261 gtacttccag cgtcatgatt tcatcgctaa tggcggcgaa ctcaaaccgc aggaaaatcc
     4321 gaacgcacaa gcgattcagg atgatttaaa agatattgat tctgaataag aaacaaataa
     4381 aaaaggtgag tcgcaaaact cacctttttt gttgttatcc ctcaattacc acaagtaaaa
     4441 ggagataaac cgacaatgac ctagacgtaa ttatgtaaaa atactgatgt tcgtcactga
     4501 ctatttccga tattcagctg ttgaaa
//
LOCUS       DBBGLQ010000214         4497 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.214, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000214 DBBGLQ010000000
VERSION     DBBGLQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.214"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..442
                     /locus_tag="ACIYSB_005244"
     CDS             <1..442
                     /locus_tag="ACIYSB_005244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432069.1"
                     /translation="PVAIIAGRMLTQPRNSDDIFSLPQCDFDGLIFHPIKNQKPLRLF
                     HAQREKYSHSVVSLQHRSGNAIYPLKRGLFPRHLRAEKGLFSCTHGSPTDPDATRAGR
                     AKSRSKKLCKFVHFRAPEREVAGGAFLLHDIGILFDNIGVKTYI"
     gene            506..757
                     /locus_tag="ACIYSB_005245"
     CDS             506..757
                     /locus_tag="ACIYSB_005245"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGC5432070.1"
                     /translation="MWTPRAIKDLRVLPARDQKSLREKVNALYGYPKFQNLDIKKLTD
                     SGGEYRLRVNHYRVRFTIEGGQIVVIRILRIQRRTSTTY"
     gene            831..1205
                     /locus_tag="ACIYSB_005246"
     CDS             831..1205
                     /locus_tag="ACIYSB_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5432071.1"
                     /translation="MSQIQFITDGAGNRVSVILPVALFEKLAGDSDLDELYEDVQNEP
                     SASPDTLYPNEVVNILSERGCTMQAAWRIYRGMTQKQVADALGIRQATVSEFERTERP
                     RRANIERLARLYGCTPEQLILE"
     gene            complement(1241..1627)
                     /locus_tag="ACIYSB_005247"
     CDS             complement(1241..1627)
                     /locus_tag="ACIYSB_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5432072.1"
                     /translation="MKARIGIIPEKVLRQRLIEIAKGERKPHPDEPRVWFSSINAAGQ
                     ALSNENISLLRLMDEEKPQTMTELAELSGRKLSNLSVTLKMLSSYGFVSLEKKGNTVH
                     PKALFTDFEIIVDPSVGSVHRATARP"
     gene            complement(1624..1998)
                     /locus_tag="ACIYSB_005248"
     CDS             complement(1624..1998)
                     /locus_tag="ACIYSB_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019454656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HGC5432073.1"
                     /translation="MNKQDFGLDTLLSMHGYQHHMDNGYWWKIEAYRVSPSPFRPHGI
                     RYNLTLHDCYNSRVFGMDNAHGIKPPKKGRFTGKLTVYDHVHRTPVDKGYPYEFVSAE
                     QLLSDFFDNVDRLINEVTKGKA"
     gene            complement(2135..2545)
                     /locus_tag="ACIYSB_005249"
     CDS             complement(2135..2545)
                     /locus_tag="ACIYSB_005249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432074.1"
                     /translation="MNRYHCVLPRHYFGQIMRSRIPAVTLRNLSTVPQPGDELEIAVA
                     GPDRSASCVTADVLNAVPVGGDWLVAIRFRQDPFAPEPLTPAGRIMQNWRDERAKANC
                     EKLINRIATTNRHAALARRIYPAKPTQPTGVKRP"
     gene            complement(2542..>4497)
                     /locus_tag="ACIYSB_005250"
     CDS             complement(2542..>4497)
                     /locus_tag="ACIYSB_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGC5432075.1"
                     /translation="RERIQKIAVLLASAVKMHLADAMDKAREITGNDKDDNLNNWLIA
                     YQYIGRRVLKLGITPPYWSALELRPDRRSPPDVTLVPGAVLRLNDAEWWNKKLRQMHD
                     VWREELLRAAGLVSRQTSVYVSREALADFREKQARTRDFLKAHDVENEDGERISLEDI
                     YYASTSNPHNRRNEMMACVKGMELIAQERGDVAFFVTVTAPSRFHSVTDAGTLNPKYK
                     GATVKDASDYLVYNFFASARKLIKKEKRGWYGIRTVEPHHDGTPHWHMLVFTSPENEE
                     RITEIMCNAAIREDRAELGDDITPRFKCEKIDPEKGTPTSYIATYIGKGIDAAAFGDT
                     DPKTGKPPVDHESGKPMADTVENAVAWARLHRIRQFQFFGLPSRQVWREFRRLAGQMA
                     RNPKGPQALTNPKADALLVAADTGCFASYIMHQGGVLIPRSEYLARTAYVTAEEPNNY
                     SEFPERIYGIRMPELGEDFTLCTHPEEWKIVRKEPEPDNRTGEGFDLQGGPAAPWTRG
                     NNCHPDEKTTNNSTQTEQSEWAKLPPLPPSSDGFVSWFKSLKRPDRKKLQRLLKKRTE
                     ESRQNASGGTDTPTDTINLGVVLPELPEIVQVRRDLEALGADFPEAMAHSVFMGARVR
                     LGDGRTVHRDEQTGRITVTSLQPEAKP"
BASE COUNT          921 a         1158 c         1272 g         1146 t
ORIGIN      
        1 ccctgtagcc atcatcgccg ggcggatgct tacgcaaccc cgcaactctg acgacatatt
       61 ctcgctgcct cagtgcgatt ttgacggcct tatctttcac cccatcaaaa atcaaaagcc
      121 cttacgtctt tttcacgctc agcgtgagaa atacagccat tctgttgtgt cactgcaaca
      181 tcgttcaggg aatgctattt accccctgaa acgcgggctg ttcccccgtc acctgcgcgc
      241 agaaaaaggc cttttttcgt gcacgcacgg atccccgacg gatccagacg ccacacgggc
      301 cggaagggca aaaagtcgtt caaaaaaatt gtgcaaattt gtgcactttc gtgcaccgga
      361 aagggaggtc gcgggaggtg cttttttact tcatgatata gggattttgt ttgacaatat
      421 aggggttaaa acctatattt aaggtgtgcc agcgggcacg gctctttaat aatttgcttc
      481 cggtgtgttt atgacgactg aaatcatgtg gacccctcgg gcaatcaaag atttacgggt
      541 tttgccagcg agggatcaga aatccttacg cgagaaagtt aacgcgcttt atggctatcc
      601 gaagttccag aatctggata ttaaaaagct cactgactcg ggcggggagt acagactcag
      661 ggtaaatcat taccgtgtca ggttcacgat tgagggaggg cagatagtgg ttatcaggat
      721 cctccgtatt cagcggagaa cctcgacaac atactaaggc ggggcgaccc gccgtttttt
      781 atcaccggca gcaaattaaa aaataaaccg atgtttagca ggaggggcaa atgagtcaga
      841 ttcagtttat tactgacggt gcgggtaaca gggtatccgt cattctgcca gtggcgttgt
      901 ttgaaaaact ggcaggagac agcgaccttg acgaactcta cgaggacgtc cagaacgaac
      961 cgagtgcatc gcctgatacg ctgtatccca acgaggtggt caacatcctc tcggaacgtg
     1021 gctgcaccat gcaggcggcg tggcgaatct atcgcggaat gacgcaaaag caggtggcgg
     1081 atgcgctggg tatcaggcag gctaccgtgt ccgaattcga aagaacagaa cgcccgcgca
     1141 gggcaaatat tgagcgcctt gccagactgt acggctgcac acctgaacag ctgattctgg
     1201 aataaaaaac agccccgacc ggggctgttt attgcacact tcaggggcgg gcggtggcgc
     1261 gatgtactga accgacggac ggatcaacga tgatttcaaa atcggtaaaa agtgccttcg
     1321 ggtgtacggt atttcctttc ttttccagac tgacaaaacc gtagctgctc agcattttca
     1381 gggttacaga caggttactc agttttctgc cggataactc cgcaagttcg gtcatggtct
     1441 gcggtttctc ctcatccata agacgaagaa gggagatatt ttcattgctc agcgcctgac
     1501 ccgccgcatt gatggatgaa aaccataccc ttggctcgtc ggggtgtggt ttccgctccc
     1561 ccttggcaat ttcaatcagg cgctgacgca ggactttttc cgggatgatc ccgatgcggg
     1621 ctttcatgct ttcccctttg taacttcgtt aatgagccga tcaacgttat caaagaaatc
     1681 agataacaat tgttctgcgg atacaaactc atagggataa cctttatcca ccggggtacg
     1741 gtgcacatga tcataaacag ttaactttcc tgtaaatctt cccttctttg gcggttttat
     1801 tccatgtgcg ttgtccatac caaacacacg cgaattgtag cagtcatgta acgtcagatt
     1861 gtagcgtatc ccgtgaggtc tgaacggact tggacttacc cggtaagcct caattttcca
     1921 ccagtacccg ttatccatgt gatgctggta accatgcata ctcaaaaggg tatcaagtcc
     1981 aaaatcctgt ttattcataa cttaagcctg aaaggttact tatgaataaa tcataacatc
     2041 tttgaacaaa tcatatcacc agttttcgtt tattcaatgt gtctttgctg cttttggtgt
     2101 ctttttgtgt ctctgtgggt tccggcgatt cttttcatgg gcgttttact cctgttggtt
     2161 gtgtcggttt tgcggggtat atccggcgcg ccagcgcggc atgtcggttt gtcgtggcaa
     2221 tcctgttaat cagtttttcg cagttcgcct tcgcgcgttc atcccgccag ttctgcatga
     2281 tgcggcctgc gggggtgagc ggttcaggtg cgaacgggtc ttggcggaag cggatggcaa
     2341 ccagccagtc accacctacg ggcacggcgt tcagcacgtc ggcggtcacg cagctggctg
     2401 aacggtcagg gcctgctacg gcgatttcca gctcatcgcc cggctgcggt accgtgctca
     2461 ggtttcgcag cgtgaccgcc ggaatacgcg agcgcataat ctgaccgaag tagtggcgcg
     2521 gcagtacaca atgataacgg ttcatggttt cgcctccggt tgcagactgg tgacggtgat
     2581 gcgcccggtc tgctcgtcgc ggtgaacggt gcgaccgtca cccagacgaa cgcgggcacc
     2641 cataaagacg gaatgggcca ttgcttcggg gaagtccgcg cccagcgctt ccagatcgcg
     2701 gcggacttgc acgatttccg gcagttccgg cagcacaacg cccaggttaa tggtgtcggt
     2761 gggtgtgtcg gtgccgccgc tggcgttctg gcgactctct tcggtgcgtt ttttgagcaa
     2821 tctttgtagt tttttcctgt ccggcctctt aagcgactta aaccagctca caaaaccgtc
     2881 cgacgatggg ggtaaggggg gcagttttgc ccactctgac tgttctgttt gtgtgctgtt
     2941 gttggttgtt ttttcgtcag ggtgacagtt attgccacga gtccaagggg cggcggggcc
     3001 gccctgaagg tcaaaaccct cgcccgtgcg gttgtctggt tccggttcct ttctgacgat
     3061 tttccactct tccgggtggg tgcataacgt gaagtcctcc cccagttccg gcatgcggat
     3121 accgtaaatg cgctcgggga attcgctgta attgttcggc tcttctgcgg tgacgtaggc
     3181 tgtgcgggcc agatattcac tacggggaat gagcacaccg ccctgatgca tgatgtagct
     3241 ggcaaaacac cccgtatcgg ctgcaacaag gagggcgtcg gctttggggt tggtcagtgc
     3301 ctgcggcccc ttcgggttgc gtgccatttg tccggcaagg cggcggaact cacgccacac
     3361 ctgacgggaa gggaggccaa agaactggaa ctggcgaatg cgatgcaggc gcgcccaggc
     3421 gacggcgttt tccacggtgt ccgccatagg ttttccgctt tcgtggtcaa cgggcggttt
     3481 acctgttttt ggatccgtgt cgccgaatgc agcggcgtca atgcctttgc ctatgtaggt
     3541 ggcgatgtag ctggttggcg tgcctttttc gggatctatc ttctcgcact tgaagcgcgg
     3601 ggtgatgtcg tcgcccagct ctgcccggtc ttcgcgaatg gcggcattgc acatgatttc
     3661 cgtgatgcgc tcttcgttct cagggcttgt gaataccagc atgtgccagt ggggcgtgcc
     3721 gtcgtggtga ggctccacgg tgcgaatgcc gtaccagccc cgcttctctt ttttgatgag
     3781 tttgcgggcg gaggcgaaga agttataaac cagatagtcg ctggcgtcct ttacggttgc
     3841 gcctttgtat ttcgggttca gcgtgcctgc gtcggtgacg ctgtgaaaac gagacggggc
     3901 tgtgaccgtg acgaagaagg ccacgtcgcc gcgttcctgt gcgataagct ccattccctt
     3961 aacgcacgcc atcatctcgt tgcggcggtt atgcgggttg gatgtactgg cgtagtagat
     4021 atcttccaga ctgatgcgtt cgccgtcttc gttctccacg tcgtgcgctt tgagaaaatc
     4081 gcgggtacgg gcctgttttt cgcggaagtc tgccagcgct tcacggctca cataaacgga
     4141 ggtctggcgg gatacaaggc ccgctgcacg taaaagttcc tcacgccaga cgtcgtgcat
     4201 ctggcgaagt tttttattcc accattcggc gtcgttcagg cgcaaaacag cgcccggaac
     4261 cagtgtcacg tccggggggc ttctgcggtc ggggcggagt tcaagggcgc tccagtatgg
     4321 tggggtgatc cccagcttca ggacacggcg accaatatac tggtaggcaa tcagccagtt
     4381 gttcaggttg tcgtctttgt cgttccctgt tatttcgcgg gccttgtcca ttgcgtcggc
     4441 aagatgcatt ttaacggcgc tggcaagcag tacggctatt ttctggatgc gttcacg
//
LOCUS       DBBGLQ010000215         4495 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.215, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000215 DBBGLQ010000000
VERSION     DBBGLQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.215"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..280)
                     /locus_tag="ACIYSB_005251"
     CDS             complement(<1..280)
                     /locus_tag="ACIYSB_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGC5432076.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMI"
     gene            complement(395..470)
                     /locus_tag="ACIYSB_005252"
     tRNA            complement(395..470)
                     /locus_tag="ACIYSB_005252"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(435..437),aa:Thr,seq:ggt)
     gene            complement(477..551)
                     /locus_tag="ACIYSB_005253"
     tRNA            complement(477..551)
                     /locus_tag="ACIYSB_005253"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(516..518),aa:Gly,seq:tcc)
     gene            complement(668..752)
                     /locus_tag="ACIYSB_005254"
     tRNA            complement(668..752)
                     /locus_tag="ACIYSB_005254"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Tyr,seq:gta)
     gene            complement(761..836)
                     /locus_tag="ACIYSB_005255"
     tRNA            complement(761..836)
                     /locus_tag="ACIYSB_005255"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Thr,seq:tgt)
     gene            1198..2148
                     /gene="coaA"
                     /locus_tag="ACIYSB_005256"
     CDS             1198..2148
                     /gene="coaA"
                     /locus_tag="ACIYSB_005256"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGC5432077.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2177..3142)
                     /gene="birA"
                     /locus_tag="ACIYSB_005257"
     CDS             complement(2177..3142)
                     /gene="birA"
                     /locus_tag="ACIYSB_005257"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGC5432078.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3139..4167)
                     /gene="murB"
                     /locus_tag="ACIYSB_005258"
     CDS             complement(3139..4167)
                     /gene="murB"
                     /locus_tag="ACIYSB_005258"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGC5432079.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDSVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQIGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1148 a         1123 c         1013 g         1211 t
ORIGIN      
        1 tgatcatgtt tttaacatag tcggcgtgcc ccgggcagtc tacgtgtgcg tagtgacggg
       61 tcggggtgtc gtattcaacg tgagaagtgt tgatggtgat accacgagct ttttcttccg
      121 gcgcgttatc gatctggtcg aatgcgcgag cagcaccgcc gtaggtttta gccagtacgg
      181 tagtgattgc agcggtcagc gttgttttac catggtcaac gtggccgata gtaccgacgt
      241 taacgtgcgg ttttgtacgt tcaaactttt ctttagacat cgattgtccc tctaagacac
      301 ggataaatcg gtgatatcac cacatcaacc aggcaacatg cccgacttgt tgaatgcaat
      361 aaacagaagg aaaaaacagg gaggagaaaa gaagtggtgc tgataggcag attcgaactg
      421 ccgacctcac ccttaccaag ggtgcgctct accaactgag ctatatcagc acatcttgga
      481 gcgggcagcg ggaatcgaac ccgcatcatc agcttggaag gctgaggtaa tagccattat
      541 acgatgcccg catcctggaa ctcggctacc tgattttcat tctgcactga atatcgagag
      601 aagctctctt tattcgagcc ggtaagcgaa cttatcgtct cgggctacgc catcgcgtgg
      661 ccgaaattgg tggtggggga aggattcgaa ccttcgaagt ctgtgacggc agatttacag
      721 tctgctccct ttggccgctc gggaacccca ccggacttga tggtgccgac taccggaatc
      781 gaactggtga cctactgatt acaagtcagt tgctctacct actgagctaa gtcggcatca
      841 agtagcgcgc attctatgga gacatgcgag ttcatgcaac taaaaaattg cataatttgt
      901 tttattggtc acattttatg cgacacgatg aagaaacagc cgaacggagg gcagattagc
      961 acactttttc aacatcattg tgctcaacaa tgcgctcctg ctaaaccata attcttttta
     1021 tcagatggaa tatctgtcac attgcttttc aacgatagct tcctggcaga gattttttct
     1081 tattattcct ccccatctgg tgttaccctc ctgcccatta acccattcaa cagaactgtg
     1141 acgcgccatg gcaaatatcg ctttgccgat agagctatga ccgccagaaa catgcttatg
     1201 agtataaaag agcaaacgtt aatgacgcct tacctacagt ttgaccgcaa ccagtgggca
     1261 gctctgcgtg attccgtacc tatgacgtta tcggaagatg agatcgcccg tctcaaaggt
     1321 attaatgaag atctctcgtt agaagaagtt gccgagatct atttaccttt gtcacgtttg
     1381 ctgaacttct atataagctc gaatctgcgc cgtcaggcag ttctggaaca gtttcttggt
     1441 accaacgggc aacgcattcc ttacattatc agtattgctg gcagtgtcgc ggtggggaaa
     1501 agtacaaccg cccgtgtatt gcaggcgcta ttaagccgtt ggccggaaca tcgtcgtgtt
     1561 gaactgatca ctacagatgg cttccttcac cctaatcagg ttctgaaaga acgtggtctg
     1621 atgaagaaga aaggcttccc ggaatcgtat gatatgcatc gcctggtgaa gtttgtttcc
     1681 gatctcaaat ccggcgtgcc aaacgttaca gcacctgttt actcacatct tatttatgat
     1741 gtgatcccgg atggagataa aacggttgtt cagcctgata ttttaattct tgaagggtta
     1801 aatgtcttac agagcgggat ggattatcca cacgatccac atcatgtatt tgtttctgat
     1861 tttgtcgatt tttcgatata tgttgatgca ccggaagact tacttcagac atggtatatc
     1921 aaccgttttc tgaaattccg cgaaggggct tttaccgacc cggattccta ttttcataac
     1981 tacgcgaaat taactaaaga agaagcgatt aagactgcca tgacattgtg gaaagagatc
     2041 aactggctga acttaaagca aaatattcta cctactcgtg agcgcgccag tttaatcctg
     2101 acgaaaagtg ctaatcatgc ggtagaagag gtcagactac gcaaataatt tgcaggggag
     2161 cgaatactcc cctttcttat ttttctgcac tacgcaggga tatttcaccg cccatccagg
     2221 gttttattat tccatcctgc tcaagtaata aagccccctg tttgtctatt ccgcgtgaaa
     2281 tgccaaatat ttctttatca ccaatgataa gtttcactgg gcgattaata aaattatcca
     2341 gcttttccca gcgcgacaga taaggtgcca atccttcttg ttcgaagagt tccaacgcag
     2401 cacgtaattc acgtattagc atggccgcca acgtattacg atcgagattg atccccgctt
     2461 cctgcagcgt gatccacccc tgattaacga cactctcttc aacacggcgc attgccatgt
     2521 tgatcccggc tccaatgact atttgcgccg catcgccagt tttgccagtc agctccacaa
     2581 gaatgcctgc cagcttgcga tcctgcagat agaggtcatt aggccattta acacgaactt
     2641 tatctgcacc cagcttgcgt aatacttccg ccatcacgat accgataacc agacttaaac
     2701 caatcgccgc cgccgggcct tgttccagac gccagaacat cgacaaatat aagtttgcgc
     2761 caaaaggcga aaaccatttc cgaccccggc gaccacggcc agcctgctgg tattctgcaa
     2821 tgcaagcatc gcccgattta agctctccga tacgatcaag aaggtactga ttcgtggagt
     2881 caatcactgg cagcacggct acactaccgc catccagctg acccaatatc tgtttagcat
     2941 taagtaactg gataggctca ggcaggctgt atcctttacc cggaacggta aagacatcaa
     3001 cgccccagtc acgcagtgtc tgaatgtgtt tattaatagc cgcccggctc attcccagcg
     3061 tttcacccaa ctgctcgcca gagtgaaatt caccgttcgc taacagggca atcaatttca
     3121 gtggcacggt gttatccttc atgaaattgt ctccactgcg ctcacttcac ctgatgcacc
     3181 aataaagcgg acttcaggct caagccagac attaaatttt tcaccaactt tctgtcttac
     3241 atgatgcgcc agctgtacaa catcttcgct ttttgcattg tcttcattaa tgagaactaa
     3301 cgcctgttga cggtgcaccg cagccccacc tatttgcatc ccttttagct ggcactgatc
     3361 gataagccaa cctgctgcca gttttactga accatccgcc tgggggtaat ttggtgctgt
     3421 tggaaattgt gacagtaatg ctttagccgt ttcggcagat acaacagggt ttttgaagaa
     3481 actaccggca ttgccattca cttttggatc agggagtttg gtggtgcgca tatgacacac
     3541 cgcattaaat acttgctgtg gcgttactgt tgtaggatcc agacgagtta agtcaccata
     3601 cgttagtaca ggttgccact cttttggcag acgcagacct acggctacaa tagcgaagcg
     3661 gtcctggtat tcatgtttaa aaatactgtc gcgatagcca aaacggcact ctttggcagt
     3721 taagcgcact tgcttgcctg tcgccagttc aacagaatca acataagcgc aaactcgctg
     3781 taattctacg ccataagcac caatattctg gataggtgat gagccgacac aaccaggaat
     3841 taatgccaga ttttccagac caggcatacc ttcctgcaaa gtgtatttta ccagacgatg
     3901 ccagttttct ccggctccta catgtaaata ccacgcatca ggttcatcat gaatttcgat
     3961 acctttgatc cggttgatga tcaccgtgcc gcgatagtcc tccagaaaaa gtacattact
     4021 tccttcaccc agaataagaa cgggttgtcc ttctgcggtt gcatactgcc aggcattgag
     4081 taattgttgt tcgtcttcgg cacatacaat gtgctgagca ttatgatcaa tgccaaatgt
     4141 gttccagggt tttaaggagt ggttcatagc tgctttcctg atgcaaaaac gaggctagtt
     4201 taccgtatct gtggggggat ggcttgtaga tatgacgaca ggaagagttt gtagaaacgc
     4261 aaaaaggcca tccgtcagga tggccttctg cttaatttga tgcctggcag tttatggcgg
     4321 gcgtcctgcc cgccaccctc cgggccgttg cttcgcaacg ttcaaatccg ctcccggcgg
     4381 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     4441 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcatg
//
LOCUS       DBBGLQ010000216         4470 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.216, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000216 DBBGLQ010000000
VERSION     DBBGLQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.216"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            47..889
                     /locus_tag="ACIYSB_005259"
     CDS             47..889
                     /locus_tag="ACIYSB_005259"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGC5432080.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            892..1980
                     /gene="capU"
                     /locus_tag="ACIYSB_005260"
     CDS             892..1980
                     /gene="capU"
                     /locus_tag="ACIYSB_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HGC5432081.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARLAKQDIEERFDINKTALKILTLAKQK"
     gene            1985..2935
                     /gene="virK"
                     /locus_tag="ACIYSB_005261"
     CDS             1985..2935
                     /gene="virK"
                     /locus_tag="ACIYSB_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HGC5432082.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIDDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            3000..3944
                     /gene="lpxM"
                     /locus_tag="ACIYSB_005262"
                     /gene_synonym="msbB"
     CDS             3000..3944
                     /gene="lpxM"
                     /locus_tag="ACIYSB_005262"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGC5432083.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMLPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
     gene            4125..>4470
                     /locus_tag="ACIYSB_005263"
     CDS             4125..>4470
                     /locus_tag="ACIYSB_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5432084.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDILNCTGVAEKYALIEQWRQQ
                     FPIEAMCQVFGVS"
BASE COUNT         1314 a          850 c         1010 g         1296 t
ORIGIN      
        1 ttaggctttc cttttatctc tacttactgg gtgccagaaa ctttaaattt taaacggagg
       61 gggcattttg tttaaagcaa atcatgttcc ggtattaatg tatcatcatg tcagccattg
      121 cccaggactg gttacacttt ctcccgagac attccggaaa caaatgaaat ggttggctga
      181 aaacaactgg aaaacactgt catcagatga actggagttt ttttatcggg gcggaaaact
      241 tccccgtaaa agcgttatgt tgacatttga tgatggatac cttgacaact ggtttcaggt
      301 atatccttta ttaaacgagt ttaatctcaa ggcacatatt tttttaatta ccagttttat
      361 tggaaatggt ccggttcgtc actctcctgg aaaggaatat tctcatcgcg attgcgaaca
      421 tcaaatagca accgggaatg ctgataatgt tatgcttcgc tggtctgaag tgaacgaaat
      481 gttgcaatct ggtctggtcg aatttcatgt tcatacacat actcatacac gttgggataa
      541 aaagttcact tcacgagaag aacagtgtaa acatcttcgg caggacttat tgagcgggag
      601 agaatattta aaaaaaatga caggaaaatg cagtaaacat ttgtgttggc cggaaggtta
      661 ttataacaag gattatattc agatcgctga agaacttgga ttccattatt tatatacaac
      721 agaaagaaga atgaatgctc ctgctaaagg aactacacgg ataggaagaa taagcaccaa
      781 ggaacgggaa aattgcgtat ggctgaaaag acgacttttt tattatacaa caccgttttt
      841 ttcatcgttg ctggcccttc ataaggggcc aagattaccg gatgactaat aatgaatata
      901 ctatttacgg aatcatcacc gaatataggt ggtcaggaat tacaggctgt tgctcaaatg
      961 aaggccctga agaaaatggg gcattcagtt ctgcttgtct gcagggagaa cagcaaaatt
     1021 gcttttgaag ccagtaagtt tggaattgat atcacattcg cgttatttcg aaacagtctt
     1081 cacattccta ctgtatggag attactcgga atagttcata gttttcagcc cgatgcaatc
     1141 gtttgtcaca gtggacatga tagtaatatt gttggtttgg tacggttttt tacctggaaa
     1201 catccattca gaattatcag gcaaaagaca tatttgacac gaaaaacaaa atttttttca
     1261 ataaatcatt tttgcgatga ggtgattgtt cccggaacaa atatgaagac acatctggag
     1321 caggaaggat gtcgaactcg ggttactgtt gtgcctcctg gctttgattt ccaggaatta
     1381 tacgttgatt cccgaaacag tttgcctccg agtgttcttt cctggctggc gtcccgaaga
     1441 ggatgccccg taattgctca ggtaggaatg ttgcgcccgg aaaaagggca cgaatttatg
     1501 ttgaatttac tgttccattt aaaaatgaat ggacgacagt tctgttggtt gattgtgggg
     1561 tctggttcac ctgaactgcg ggaacattta cagtatcaga ttgacagtat gggcatgcat
     1621 gatgatgttt ttattgctga caatgttttt cctgccgccc ccgtatatcg ggttgccagt
     1681 ctggtggttc tgccttcaga aaacgaatct tttggtatgg tgctggcaga agcatcggca
     1741 ttttctgtgc ctgtagtggc cactcagatt ggtggaatcc ctgaggttat tcagaacaac
     1801 cagaccggga cattgttacc agcaggtaat aagcacgcat ggatgtgcgc cctgaatgat
     1861 ttttttaatg accctgggcg tttttatcag atggctcgcc tggcaaaaca ggatatagaa
     1921 gagcggtttg atattaataa aactgcgtta aaaatactca cattagcgaa gcaaaagtaa
     1981 caatatgttt tctataagta acttatcatt tatcggtttc cttaaaagga ttattttttc
     2041 ctcagattca ctaccgggga agtgggaaca cagaaaattt cggttcatgt acattttgcg
     2101 atgttctata aatccggttg tcagtattcg atattattat gaactgcgtt ccttgccgtg
     2161 cattgatgat attctggcaa tacaccccac gttgccagca agaattcatc gcccttattt
     2221 gcataaaggg gggcgcgcct ggacccgtgg acaatatatt cttgagcatt atcgctttgt
     2281 gcaaaatttg ccagaaaaat attctaaatt tttgtttcca caaaaatcgg tatcattagt
     2341 ccaattcatc gggaaagatg gagagaattt tgatatccag tgttctccca gtggttttga
     2401 ccgggaagga gagttgatgt tgtcattgtt ttacaataaa acagttattg cccggttaac
     2461 attttcagtc attctgactc aaaatggaca cattgctttt attggcggat tacagggagc
     2521 ccctaaaaat accggacctg atgttattcg gtgcgcaacc cgggcctgtt atgggatttt
     2581 cccaaaacgg attatttttg aagcattttg tgcgttgatg aaagcttgca atatctctga
     2641 gtgtctggca gtcagtgagc acagtcatgt tttccggcaa ttgagatact ggtatcagaa
     2701 acgcaaaacc tttgttgcgg tctatagtga tttctgggag tctgtagcag gaaaaacctg
     2761 tggtgactgg tacagattac caacccaggt gattcgaaag ccgctaagcg atatagccag
     2821 taaaaaacgc tctgagtacc gaaaacgata cgctttgctg gattatatcc atgaaactac
     2881 tatccgctct ttggatgcat atcctgtaca ctcagaacat cagaatttaa attaaggttt
     2941 gatgtattct ctgaataaaa cagtaatgat aattttggta gaggcattcg cactaaataa
     3001 tgaaaaaata taaatctgag tttattcctg aatttaagaa aaattacctt tcccctgttt
     3061 actggtctac atggttcctt ttgggaatga ttgcaggtat ttcaatgctt cccccttcat
     3121 tcagagatcc tgtcttggca aaaatagggc gttgggcggg cagattgagc aaaaaagctc
     3181 gtcgcagggc gacgattaat ttatcgcttt gtttcccaga aaagagtgat acagaacggg
     3241 aaataattgt cgacaaaatg tttgccacag cattgcaatc catagtgatg atggcagaac
     3301 tggcaattcg tggtccggaa aagttccaga aacgagtgtt ctggaaaggc ctcgaaattc
     3361 ttgaggaaat tcgacacaat aatagaaatg tgatttttct ggttccccat ggctggagcg
     3421 tggatattcc tgcaatgttg ctggcagctc agggaaaaaa aatggccgcc atgtttcatc
     3481 agcaacgaaa tccagtgatt gattatgtct ggaattcagt acggcgtaaa ttcggggggc
     3541 gcttacatgc ccgggaggat gggataaaac catttattca gtcagtacgc cagggatact
     3601 ggggatatta cctcccagat caggatcatg gtcctgaata cagtgaattt gctgattttt
     3661 ttgcgaccta taaagcgaca ttaccaatca ttggacgtct gatgaacatc agtcaggcta
     3721 tgattatacc acttttcccg gtttatgatg aaaaaaaaca tctcctgact attgaaattc
     3781 ggccaccaat ggatgcttgc attgccagcg cggacaataa aatgattgcc cgacaaatga
     3841 acaaaacagt ggaaattttg gtggggccac atccggaaca gtatgtctgg gttttaaaat
     3901 tgttaaaaac acgcaaatca aacgaagcgg acccgtaccc ttgaaattat aaagtacaag
     3961 tatttccact agttgtttgt aagcgtacag cctgaaccgt ctggtcagaa tctgacgaat
     4021 tagacaaagt ggtgtccacc aaataagtag tgggaaccaa tgaggtagcc tgagtttaac
     4081 ggacactcct tcctgaaata gaatggcatc agaaggagct aataatgagc agaaaaaccc
     4141 aacgttactc taaagagttc aaagccgaag ctgtcagaac ggttcttgaa aatcaacttt
     4201 cgatcagtga aggtgcttcc cgattatctc ttcctgaagg cactttagga caatgggtta
     4261 ccgccgccag aaaagggctc ggtactcctg gttcccgcac ggtggctgaa ctggaatctg
     4321 aaattctgca actgcgtaag gcgttaaatg aagctcgcct tgagcgagat atattaaatt
     4381 gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa tttcccattg
     4441 aagcgatgtg tcaggtattt ggtgtatcca
//
LOCUS       DBBGLQ010000217         4295 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.217, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000217 DBBGLQ010000000
VERSION     DBBGLQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.217"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            663..1937
                     /locus_tag="ACIYSB_005264"
     CDS             663..1937
                     /locus_tag="ACIYSB_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HGC5432085.1"
                     /translation="MLAEYERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRN
                     DRAISEARNKLSSVTESLNTARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNH
                     SIVVSGDPRFAGTIKITTSALIDNRANLNYLLTHSGLDYKRNILNDRNPVVTEDVEGD
                     KKIYNAEVAEWDKLRQRLLDARNKITSAESAVNSARNNLSARTNEQKHANDALNALLK
                     EKENIRNQLAGINQKIAEEKRKQDELKATKDAINFTTEFLKSVSEKYGAKAEQLAREM
                     AGQAKGKKIRNVEEALKTYEKYRADINKKINAKDRAAIAAALESVKLSDISSNLNRFS
                     RGLGYAGKFTSLADWITEFGKAVRTENWRPLFVKTEAIIAGNAATALVALVFSILTGS
                     ALGIIGYGLLMAVTGALIDESLVEKANKFWGI"
     gene            complement(1959..2294)
                     /locus_tag="ACIYSB_005265"
     CDS             complement(1959..2294)
                     /locus_tag="ACIYSB_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1A immunity protein"
                     /protein_id="HGC5432086.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFLLSLPLSMIYILS
                     VIIKRLSVR"
     gene            2423..2770
                     /locus_tag="ACIYSB_005266"
     CDS             2423..2770
                     /locus_tag="ACIYSB_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HGC5432087.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(2790..3179)
                     /locus_tag="ACIYSB_005267"
                     /pseudo
     CDS             complement(2790..3179)
                     /locus_tag="ACIYSB_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3332..3784
                     /locus_tag="ACIYSB_005268"
     CDS             3332..3784
                     /locus_tag="ACIYSB_005268"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGC5432088.1"
                     /translation="MKFMECTVRDVIYGTNVRIVKPVNIYKCELRDNVFVGPFVEIQK
                     GCVIGRGSRIQSHTFICENVTLGENCFIGHNVTFANDLFHSGAPDPSPDNWISIILGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKNIEKKGIYAGNPARLIRTL"
     gene            complement(4173..4295)
                     /locus_tag="ACIYSB_005269"
                     /pseudo
     CDS             complement(4173..4295)
                     /locus_tag="ACIYSB_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         1210 a          967 c         1083 g         1035 t
ORIGIN      
        1 catgtataca agcagttatt tcatttgttt tcctcagcgg atgaaggaga taccgaatgt
       61 ctgaccctgt acgtattaca aatcccggtg cagaatcgct ggggtatgat tcagatggcc
      121 atgaaattat ggccgttgat atttatgtaa accctccacg tgtcgatgtc tttcatggta
      181 ccccgcctgc atggagttcc ttcgggaaca aaaccatctg gggcggaaac gagtgggtcg
      241 atgattcccc aacccgaagt gatatcgaaa aaagggacaa ggaaatcaca gcgtacaaaa
      301 acacgctcag cgcgcagcag aaagaaaatg agaataagcg tactgaagcc ggaaaacgcc
      361 tctctgcggc gattgctgca agggaaaaag atgaaaacac actgaaaaca ctccgtgccg
      421 gaaacgcaga tgccgctgat attacacgac aggagttcag actcctgcag gcagagctga
      481 gagaatacgg attccgtact gaaatcgccg gatatgatgc cctccggctg catacagaga
      541 gccggatgct ctttgctgat gctgattctc ttcgtatatc tccccgggag gccaggtcgt
      601 taatcgaaca ggctgaaaaa cggcagaagg atgcgcagaa cacagacaag aaggccgctg
      661 atatgcttgc tgaatacgag cgcagaaaag gtattctgga cacgcggttg tcagagctgg
      721 aaaaaaatgg cggggcagcc cttgccgttc ttgatgcaca acaggcccgt ctgctcgggc
      781 agcagacacg gaatgacagg gccatttcag aggcccggaa taaactcagt tcagtgacgg
      841 aatcgcttaa cacggcccgt aatgcattaa ccagagctga acaacagctg acgcaacaga
      901 aaaacacacc tgacggcaaa acgatagttt cccctgaaaa attcccgggg cgttcatcaa
      961 caaatcattc tattgttgtg agcggtgatc cgagatttgc cggtacgata aaaatcacaa
     1021 ccagcgcgct catcgataac cgtgcaaacc tgaattatct tctgacccat tccggtctgg
     1081 attataaacg caatattctt aatgaccgga atccggtggt gacagaggat gtggaaggtg
     1141 acaagaaaat ttataatgct gaagttgctg aatgggataa gttacggcaa agattgcttg
     1201 atgccagaaa taaaatcacc tctgctgaat ctgcggtaaa ttcggcgaga aataacctca
     1261 gtgccagaac aaatgagcaa aagcatgcaa atgacgctct taatgccctg ttgaaggaaa
     1321 aagagaatat ccgtaaccag cttgccggca tcaatcagaa gatagcggaa gagaaaagaa
     1381 aacaggatga actgaaggca acgaaagacg caattaattt cacaacagag ttcctgaaat
     1441 cagtttcaga aaaatatggt gcaaaagctg agcagttagc cagagagatg gccgggcagg
     1501 ctaaagggaa gaaaatacgt aatgttgaag aggcattaaa aacgtatgaa aagtaccggg
     1561 ctgacattaa caaaaaaatt aatgcaaaag atcgtgcagc gattgccgca gcccttgagt
     1621 ctgtgaagct gtctgatata tcgtctaatc tgaacagatt cagtcgggga ctgggatatg
     1681 caggaaaatt tacaagtctt gctgactgga tcactgagtt tggtaaggct gtccggacag
     1741 agaactggcg tcctcttttt gttaaaacag aagcgattat agcaggcaat gccgcaacgg
     1801 ctcttgttgc actggtcttc agtattctta ccggaagcgc tttaggcatt atcgggtatg
     1861 gtttactgat ggctgtcacc ggtgcgctga ttgatgaatc gctagtggaa aaagcgaata
     1921 agttctgggg tatttaaata aaatataaga caggctgtct atcttacaga cagccttttt
     1981 ataataacag aaagaatata tatcatactt aacggaagag ataataggaa aacagtaccc
     2041 gtataaacag caagcaatcc catttttccg ggagtatcag caaaaaaacc gctgttccag
     2101 aaatcaggcc gggtaaattt cagagctgta tcttcaatat accattttgc aaccggatac
     2161 aaaaccattc cgcagagaga aatacaccag aacagtaatc tgtatttaaa atcataatcc
     2221 catgacatat acagcatata tccccccgtc acccatcccc accacatatt attaaaataa
     2281 tattttctgt tcatcggcct gttttccttt cttttgcact tttgtcagtg tactgatgca
     2341 tgacaacata accacagcat gataataata atcaataaca ataagctgtg tcacgtttac
     2401 atcatggaac ggaggtgctc ctgtgacttt tgagcagaaa aaagcccggg ccattgccct
     2461 gatggacagc aaaaagatgt ggcggagcaa ttatgcccct cctcttctgc gtattctatg
     2521 gcggctgggt atccggcttc cgccattacc ctttatgccg ttctggcagg tcacggtgct
     2581 gacggggggc ttatggggca tttcctgggg atgtgcaatg tggtttattt actgggggcc
     2641 gtcaggaatg gtggcaggcg aggccattat tataagtatc acgggtggtt ttttgtccgg
     2701 actgtgcatg gcctctttcc actggtggcg caggaaagtt aaccggctgc caccatggga
     2761 tgatgtgtaa accgtgcaca ggcgtgttct tacggagcgg cactttcatt tatctgtacg
     2821 cgaacctcca ttcctgcatc gtgacatgcc tgcagccact gcgccacttc cagcggatcg
     2881 ccctcccggc gtaccactct gccttcttta ttccataact gcagacaggt gctgccgtcg
     2941 agacgcacca caaaatcccc acggcaggcc tgatacgtta tgccgttcag ctctaccgcc
     3001 agatcagtgg cagaagtgcc gtctgcgggc agacctgctg ccgggaccag cggcagtacg
     3061 acgacttctg ccgcacgctg ttctgcccgg aggcggtcct tttctgccag cagcagaggc
     3121 tgtgccacgg cccggccggc gtccgtcagt tccactacca actgcgtgtt tcccggcgcg
     3181 cagatcaaat ttctggtgga agtggagcca gagctgattt tttgctgggc atcggtaaaa
     3241 gtgcggctta taagctattc tgaaatattg agagtgaatc tgggcggtat aattccggca
     3301 agtgtcatct gtatttaaag gacgtgtgag tatgaaattt atggaatgta ctgtccggga
     3361 tgtgatttat ggaaccaatg tacgtattgt taaaccggtc aacatttata aatgtgaact
     3421 cagggacaac gtgtttgtcg gacccttcgt tgagatacag aaaggctgtg tcattggtcg
     3481 tggaagccgg atacagtcgc acacgtttat ttgtgagaac gtaacactgg gtgagaactg
     3541 cttcattggt cataacgtga cgttcgccaa cgatctgttt cactcaggag caccagatcc
     3601 gtcaccggat aactggatca gcattatcct aggggattcg gttactgttg gcagtggagc
     3661 cattattctg tctccgtata tatgcagcgg agctgttatt ggtgcggggg ctgttgtggt
     3721 aaaaaacatt gagaaaaaag gcatttatgc aggaaacccg gcaagattga tcagaacatt
     3781 atgaaaaaga aaaggggcgt tactggccct ttttatcaga ggtaggtctg tgaatacggc
     3841 aggtgtacag aaatcgattg caggggatcg atttccagag ccgcccgata ttctgcgtca
     3901 gtgatatcac cttttttcag taaataatcg agagcattgt ttcccagccc tgacagtatg
     3961 cgtattctgg cgcatccgaa aatcaaacag gctgtccatc ataccatgct ctggaaatac
     4021 gggagggctg gccagatagg caggtttgac aatacttctg atggtgttcc agtatcggga
     4081 aaagtcgcgg gtcgggtccg ggagaagacc tgacagcagc ccaatccgga tggtttccaa
     4141 ccaactgtca taaaatcact taacatgaag tgaagctgat taacatgggc gaaactgtcg
     4201 aatcggcggt accggacgaa gaagttctcc ttgaggtatt tcaccatccg ttccacctta
     4261 actttggttc tggcccttcg tggacggcat gcccg
//
LOCUS       DBBGLQ010000218         4253 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.218, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000218 DBBGLQ010000000
VERSION     DBBGLQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.218"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..505)
                     /locus_tag="ACIYSB_005270"
     CDS             complement(<1..505)
                     /locus_tag="ACIYSB_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HGC5432089.1"
                     /translation="MSVLLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFARKINFPVETPCFQIKQTELEGADALPHWLPLQKIANGAVGHCMGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGS"
     gene            complement(609..737)
                     /locus_tag="ACIYSB_005271"
     CDS             complement(609..737)
                     /locus_tag="ACIYSB_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432090.1"
                     /translation="MADLLLSILVPVDAGKYRKGGSVPSQNMVRGIVRRLVIVGMA"
     gene            1185..1376
                     /locus_tag="ACIYSB_005272"
     CDS             1185..1376
                     /locus_tag="ACIYSB_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGC5432091.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(1429..1662)
                     /locus_tag="ACIYSB_005273"
     CDS             complement(1429..1662)
                     /locus_tag="ACIYSB_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432092.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1758..2381)
                     /locus_tag="ACIYSB_005274"
     CDS             complement(1758..2381)
                     /locus_tag="ACIYSB_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGC5432093.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTFRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            2781..3824
                     /gene="asrA"
                     /locus_tag="ACIYSB_005275"
     CDS             2781..3824
                     /gene="asrA"
                     /locus_tag="ACIYSB_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HGC5432094.1"
                     /translation="MAVKITPEEFSLLIQRLNTRWRVLAPSAEFRGGRFADTDNIIYQ
                     QIRGWDDLVWQEKSHMSPNTIISPITETLFYFNKDTIQIAETDTTPVLIFARACDINA
                     MSRLDYMYLSNGNNSDYSYQRLRDHIRFVLIECPQSFENCFCVSMGSNKTDNYSAAMR
                     FSDDGASVFIRDAFFEEALQGMGQRTEYEPVFVSENPEQVTLPEKICQSPQSVRDIII
                     NHPLWDEYNSRCIGCGRCTTGGPTCTCYSVFDVAYDENPQRGERRRQWASCMVPGFSD
                     MAGGHRFRQSAGERLRYRALHKVNDYKARNGTEHMCVGCGRCDDRCPQYIKFSRIINK
                     MTAAVRQALSGEA"
     gene            3828..4097
                     /locus_tag="ACIYSB_005276"
                     /pseudo
     CDS             3828..4097
                     /locus_tag="ACIYSB_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461484.1"
                     /note="with AsrAC catalyzes the reduction of sulfite to
                     hydrogen sulfide; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
BASE COUNT         1141 a         1063 c          966 g         1083 t
ORIGIN      
        1 gagagccttc gtgtgccgga aatgaggaat acaactgaat atagtcatca ctgtccggtg
       61 tcagacgcac atgccgcaca acaccgggaa taataaccag ccggaggata ccgcttttta
      121 aatcctgcga aggtgccaga acccgggtag tgacataacc atgatcgacc agacggttct
      181 gcaatgtact catcagcaga ttaattcctt tcgcccccat gcaatgcccg accgccccgt
      241 tggcgatttt ttgtaaaggc aaccagtgtg gtaacgcatc agccccctcc agttctgtct
      301 gtttaatctg aaaacagggc gtttcaacag gaaaattaat cttacgggca aaagagccag
      361 gtgcagaaag gcgaacatca ggggccgaag gcgtcagttg ttgctcaagg gcttcctgac
      421 gttgctgctg aatgataaat gcttcatctc cgctacgccg gacatccgct gcctgaagag
      481 ataaaggcga taataaaaca gacatcagtg tcagtgacag caaggacgga cagcgccggg
      541 ataagctttg ttcctcagcc ctctcgttcc tgcaacataa ttttccagtc aaacgaaaaa
      601 ccttttattc atgccatacc aacaatcacc agcctcctga cgatacctct caccatgttt
      661 tgcgatggca cgctaccgcc cttccggtat ttacccgcat caaccggtac cagaatgctg
      721 agtaataaat ctgccaattt ttagctgact tatttttgat cattatagaa taatgatctg
      781 aactacagca atatgacaga tttatcgatc gatataacac aaatgaacag ttctttattc
      841 tgtcacatat cgtaaaaaca gcacattgca aaatttataa ccataattaa tggaaaagtg
      901 cagaaaatta ttccactgaa tgcttttaat acagtcggtt agcttaaaaa atagcaattt
      961 ttgatttcag tcattgaaaa ttgcaatgcg tgacgattca acagcattta agtgagtttc
     1021 aggatatggg aaggacatga aaggaggagc gcaataaaat acacaggaag aagggggaaa
     1081 tgtttcgtaa aaacagaaaa ttaacagcgc acatcaggaa acgcagtctc ctgattcagt
     1141 gctaccacat gaaaatttag ttaactgtta ccgtactaaa atttatgtca gaagatgttc
     1201 cattaccgaa agtcaatcag cgatacaaag acgatcatgg tgcacttgtc accgtgacca
     1261 gtgttgagga aacccgggtg gtgtttatgc gggacggcta tccgcatccc tgcatgcgac
     1321 cgatgtacaa ctttctggga aaattcaaac ccgaaccccg ggaagaaaca gaataaaacg
     1381 aaatccctgc cggcaatcac actgcctgat ggcagcataa tccacttatt aattctcatc
     1441 cccggagaca agccctgaaa accactccgg gtgttcactg tatgctgtgt tcagcgcatc
     1501 ccaggaaacg ttgatataaa agctgtcctg caatttccgg gtaaccacct ctgccaggcg
     1561 gttccggtta tccaccagtc cggcataacc tctctctgcc agccactcat tcagttcatg
     1621 ttccagagac agattgataa tatcgtaatc ggaccttgcc attcccgttt ccttagcaca
     1681 tgttttcagg cacggggata aagtataaag aatgtcgtac tgctgctgcc agagcaatat
     1741 tccccctgat ggttgtatca gagatggtat gccggtactt tgcgggttgt tcctgtcagg
     1801 ttatcgtgca cctccacggt cgtggtcacc accggcattc cggcctttct cagcctcaga
     1861 acatcagccg caatacgctg actgccagac cagaacaggc cgtccagtgc agtcaccagt
     1921 aaacccgcct ccagtgcata cttcagccgt tcacggggcg ctttcacttc ccgggcaacc
     1981 tgctgatatg gcaatgatgt gttttcattc ccaaccacgc gacgaatacg gtgagacaga
     2041 tgataccgga atgtatccgg cacaccagaa aggctggcct tcaggctgta cacgcaacca
     2101 aaccgtttat cgttaaacac cacatttttc tgactgatgt cccattcttc acgcagcgcg
     2161 gcaatcagtt gtggtgtccg ggtaagcaac aagcgaaaag gcagttcaaa actggtgacg
     2221 taatccacat tcagcagtgc aatacgcagc cgttcttctg gcccggcctc tgtctgccgg
     2281 cactcctcca gaacatcctg ccactgccgg cgaagacggg gggattcgtt ccgttctgta
     2341 aaacagtatt tatccgccag ataatcaatt cgtgtacgca tactgaaaag agttccgtat
     2401 aaaaattcat ctgacaaaac tttatcaatc gctgatgaac agggaaagca taaatcgccc
     2461 ggtcagatac tcctgtctgc ttccgctctg ccatatatct tcgttaatcg ctcaccgttg
     2521 ccaccttgtt tccggcatat ttcatgcagc catgtgttcc cgtcaccgta ccgcatgcgt
     2581 gaacgaaaca gcatttgttg tttcggtgga cgtcgtacaa cggtgttact ccatgtgggt
     2641 acctgagtca ccacttatta attatagcga taacaaaaat ctgttttttc cgttgttact
     2701 gttaacgctt cagcgttagc gtaaaaaaag aagcattatt ttctcaatat attcttcact
     2761 gacaggtaat ggagtatgtg atggcggtta aaatcacgcc tgaagagttc agtctgttaa
     2821 tccagagact gaacacgaga tggcgcgtcc tggcaccttc tgccgagttt cgcggcgggc
     2881 gttttgctga tacggacaac atcatttatc agcaaatccg gggatgggat gacctggtct
     2941 ggcaggagaa atcccacatg tcacccaata cgattatttc accgataaca gagacgctct
     3001 tttattttaa taaagacact atccagattg cggaaacaga taccacacca gtacttatat
     3061 ttgcccgcgc ctgtgacatt aatgccatgt cgcgactgga ttatatgtat ttatcaaacg
     3121 ggaataattc tgattacagt tatcagcgac tgcgcgacca tattcgtttt gttctgattg
     3181 agtgcccgca aagttttgaa aactgtttct gtgtctccat ggggagtaat aaaaccgata
     3241 attacagcgc ggcaatgcgt ttcagtgatg acggcgccag tgtttttatc agggatgctt
     3301 tttttgaaga agccttacag ggaatgggac agcgcacgga atatgagcct gtcttcgtca
     3361 gcgaaaaccc ggagcaggtt accctccctg aaaaaatctg tcagtccccc caaagcgttc
     3421 gcgacattat tatcaatcat ccgctctggg atgagtataa cagtcgctgt attggttgcg
     3481 ggcgctgcac aaccgggggt ccgacctgta cctgctacag cgtttttgat gtggcttatg
     3541 acgaaaaccc tcagcgtggt gaacgccgcc gccagtgggc cagctgtatg gtaccgggtt
     3601 tcagcgatat ggccggcgga caccgttttc gccagtctgc gggagaacgt ttacgttacc
     3661 gtgcacttca taaagtcaat gattacaaag cccgcaatgg tactgagcat atgtgcgtgg
     3721 gctgcggacg ctgcgatgac cgttgtcctc agtacattaa attctcacgt attatcaata
     3781 aaatgactgc cgctgtccgg caggcactct ccggggaggc ataacctgtg tcaacgtgtt
     3841 cctgtcacga taaagaaaag cacagccttt tgcctgctgc ataccgcatt atttccatca
     3901 cccgtcatac accacaggag tggaatttcc gtgtggaagt cgattttccg gcccgctggg
     3961 gacagtttgt cgaagtctcc ctgccatgcg tcggggaggc gcccatttct gtttctgact
     4021 gcggagaggg ctggatagac ctgcttatcc gcaatgtcgg caaagtgacc ggcgcgcttt
     4081 ttaccctcca tgagggctgc gtattttcat gaaaggagat cactcaataa cttccatcga
     4141 gatcgggtaa taaccccctg tgtcagcata gttgtcgcct agatttttac acaggagaga
     4201 gactatgccc gcacatgcag taccaattga tataaaagaa caaggtgtta cct
//
LOCUS       DBBGLQ010000219         4243 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.219, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000219 DBBGLQ010000000
VERSION     DBBGLQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.219"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(58..990)
                     /gene="glsA"
                     /locus_tag="ACIYSB_005277"
     CDS             complement(58..990)
                     /gene="glsA"
                     /locus_tag="ACIYSB_005277"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGC5432095.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEDGNSVRGQKMVASVAKQLGYNVFKG"
     gene            complement(1270..2283)
                     /locus_tag="ACIYSB_005278"
     CDS             complement(1270..2283)
                     /locus_tag="ACIYSB_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5432096.1"
                     /translation="MSIKKLEDGRYFVDIRPDGSKGRRIRRKFEKKNLAVAFEREVLA
                     DRPSVDVFGNLLDKRYLSDFIATWWLLIGRHKDYANRRLNNLQCICLDMGDPMMYEID
                     ERMVAEYRSRRLDQGVKASTINHDLFAFSAALKAMAEIGEYHGSNPVLSLPRLKEKTP
                     EMAYLNHEEIGAFLSICKGSGDYYRMAVLLLSTGCRWNEAYQLMGEHIIGNKVVFNFT
                     KNNKRRVVPVSDDVVRLVKHRKEGRLFRVSYKWFRLKLKVAKPNLPDGQAVHVLRHTF
                     ATHFMMNGGNIISLQKILGHADISQTMTYAHFSPDYLSDAVMHNPLCDMSIECPHFEE
                     KGR"
     gene            complement(2353..2652)
                     /locus_tag="ACIYSB_005279"
     CDS             complement(2353..2652)
                     /locus_tag="ACIYSB_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5432097.1"
                     /translation="MSNTQGEKLAIMRDSERLTKQQFVDLVGLNYTTYHGYERDKSKM
                     TLESAIKIFGHPRFHKYQNWFMYDRIDPEHGQIAPALAHCGHVETTSPQSGKQTG"
     gene            2777..3265
                     /locus_tag="ACIYSB_005280"
     CDS             2777..3265
                     /locus_tag="ACIYSB_005280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432098.1"
                     /translation="MAKQRDFEQITTHQHCRLYPLDGYITMNVCKMSSTQAGRIRDMS
                     LSDLQQQEFVSPEEFAYITGRTYKAAKNIMDRNESLLIKEYATPDATRPKRFIRLQHY
                     WAAIINNKASLTPREHLFINEIRNNKTLYREMMKINLKIREGREVSKKKPRYSHQSQS
                     AA"
     gene            3641..3949
                     /locus_tag="ACIYSB_005281"
     CDS             3641..3949
                     /locus_tag="ACIYSB_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HGC5432099.1"
                     /translation="MRKHYSQPYIRNSAPRSNNRWSLRETHATPGGHVVKQTGEHTWL
                     IEQAGLVIHRCNRNPFTGNRIFALSSGDNQFGQDLTLHEALRTADRLIAGRSFQHPDY
                     "
     gene            4008..>4243
                     /locus_tag="ACIYSB_005282"
     CDS             4008..>4243
                     /locus_tag="ACIYSB_005282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432100.1"
                     /translation="MKRAYKEIANDILQNYHLTLASNGVAYDEQSFRAMQGGILGLYI
                     AATASGDLEALAELKVIYPKELGEDLIKPYLIEEL"
BASE COUNT         1264 a         1068 c          883 g         1028 t
ORIGIN      
        1 cgacgttcca tagaccgaga ggtttgttac cgttattacc ttccgtgttc atcatgatca
       61 gcccttaaac acgttatagc cgagttgctt agcgaccgat gccaccattt tttgaccgcg
      121 aacactgttg ccatcttcgt ccagcggtgg tgagaacgcg gcaattccca tcactccagg
      181 gacgaccgcc agaataccgc cacctacacc gcttttgccc ggtaaaccaa cacgatacgc
      241 ccagtcaccg gagcgaccat acagcccttc catcatcatt tcggccagaa tgtacggcac
      301 gttgtcggcc tgaagaacgc gtttatgcgt caacggattc acaccacctg ccgccagcgt
      361 cgcgccaagc gttgccagtt caatagtatt gaggagcgtg gagcactgac gggtatacac
      421 gtcacaggct tccattgcat cacaatagag atatccggcg gagtacagca gccaggctat
      481 ggcccggtta tggaagttgg ttgtttgttc cgactggttg acttcgtcag agagcgctac
      541 ctgctcgcca gccagttgct gttggatatg taaaattcgc tgccagcgtt gttcaacatt
      601 ttcagcgtta atcaggctgg tggtggcaat agcgccagca tttaccagtg gcgaaagcgg
      661 tttgccgcca tgcaactcta aggcgataac tgagttaaag ggcaatccgg tcgggtcagc
      721 gccaattttg tcctgtaccg cctgcgggcc gacatcttct aacgcaaggg ctaacgtaca
      781 gactttcgag atggattcca gtgcaaagcg gtaatcactg tcacccgcac tatagacgtt
      841 gccatcgcag gtcacgatag ccactgccgc cagttgacct ggtacattcg ccagaaaggg
      901 aatgtaatcg gcattttgtc cgccgttaag tgagtgaaat tgggtgtaag cctgatccac
      961 tgcctgctgt aatttgtttg catctaacat cttttgttaa ctccttttta tagatgcggg
     1021 aggtaattcc tcaccccggt gccgattttc aggcatcctg atttaactta gcacccgcaa
     1081 cttaactaca ggaaaacaaa gagataaatg tctaatcctg atgcaaatcg agccgatttt
     1141 ttaatcttta cggactttta cccgcctggt ttattaattt cttgaccttc cccttgctgg
     1201 ggacaaagaa ctgcgtggtg taaaataaca ccacgcaata tcaataagtt acataaccac
     1261 tttaactctc tacctccctt tttcctcaaa atgtggacac tcaatggaca tatcacatag
     1321 cggattatgc atcacagcat cgcttaaata atccggcgaa aaatgcgcat aagtcattgt
     1381 ctgagatata tctgcatgac cgaggatttt ttgaagagaa ataatattcc ccccattcat
     1441 cataaaatgg gtggcaaatg tatgcctcaa tacatgaacc gcttgaccgt ccggcaaatt
     1501 aggcttagcc accttcagtt tcagcctaaa ccatttataa ctcacccgaa ataaacgccc
     1561 ctccttgcga tgttttacaa gcctaacaac atcatcagaa acaggaacaa cccgacgctt
     1621 attatttttt gtgaaattaa atacaacctt attaccgata atatgttccc ccattagctg
     1681 atatgcttca ttccagcgac agcctgtaga caacaaaagg acagccattc ggtaataatc
     1741 tcccgaaccc ttgcagatag aaagaaacgc accaatctct tcatgattaa gataagccat
     1801 ctctggggtt ttttccttga ggcgaggaag cgacaataca gggttactac cgtgatattc
     1861 accaatctct gccatagcct ttaacgcagc gctaaacgca aaaagatcat ggttgattgt
     1921 agatgcctta accccttgat cgagacggcg cgatctatat tcagcaacca tgcgttcatc
     1981 aatctcgtac atcatagggt cacccatatc aagacaaata cactggaggt tattcagtct
     2041 acgattcgcg taatccttgt gacgacctat cagtaaccac cacgtcgcaa tgaaatcgct
     2101 taaatatcgc ttgtccagaa gattaccgaa aacatcaaca gaagggcgat cagcaagaac
     2161 ttcgcgttca aatgctacag ccagattttt tttctcaaac ttccgacgaa tgcgccttcc
     2221 cttacttccg tcgggtcgaa tgtccacaaa atagcgacca tcttcgagct tcttaattga
     2281 cataagaaga ccctccaaca gtccattctc catcttgaaa acagatagag aaaacgtatt
     2341 ccttataaag agttaaccag tctgtttccc tgattggggt gacgttgttt cgacgtgccc
     2401 acagtgtgcg agagccgggg ctatttgccc gtgttctggg tcgatgcggt catacataaa
     2461 ccaattctga tacttatgaa aacgggggtg accaaatatc ttaattgcag attccaatgt
     2521 catcttagat ttgtcacgct catagccgtg ataggttgtg taattaagcc ctactaaatc
     2581 aacaaattgc tgttttgtta gcctttccga atctcgcatg atcgctaact tttcaccttg
     2641 agtatttgac atatttacga gtactccata tagttacgca aaaacaacat tcctagccta
     2701 aaacggcaaa acaccgtcaa aggccgatta ggctaagcaa gcctcaatca atggcaacct
     2761 tggagtgtat caaaaaatgg caaaacaacg agattttgaa caaatcacga cacatcagca
     2821 ttgccgtctc tatccattag acggctacat cacgatgaac gtgtgcaaaa tgagttcaac
     2881 gcaggctgga cgcatacgtg atatgagtct gagcgactta caacaacagg agtttgttag
     2941 cccagaggag tttgcataca tcacggggcg cacctacaaa gcagccaaaa acatcatgga
     3001 ccgaaacgaa agcctgctta ttaaggaata cgccacgcca gacgcaacac gacctaagcg
     3061 atttatcaga ctccagcact actgggccgc aattatcaac aacaaagcat cgctgacacc
     3121 acgcgagcat ctatttatca atgagattcg aaacaacaaa acgctttacc gcgagatgat
     3181 gaaaatcaat ctcaagattc gcgaagggcg agaagtctcg aaaaaaaaac caagatactc
     3241 ccatcaatcg caatcagcgg cataacacca aaaggagttt tcccatgtgc gacctgagca
     3301 aagcagagct aattgcaatc atattgtttg ccatagattt tggttttctt ttgagcggcg
     3361 caattctcct tcctgcaatt aacaggaaaa attaatcacc actgcaaata acaagagcca
     3421 acaacatgac cccaagacaa gtattaatct ccaaaaatga tgacatattg cactttcaag
     3481 taaaatacat aatagccaca acaaaggtag gaacatcaat ctatatacac tatcagaatt
     3541 tctatctgga gagagtcttc gaaacggaag gagaagccca gaagtttttg atacacataa
     3601 aagattgcat agaaggaaaa ataagaccag gggatcaata atgcgaaaac attattcaca
     3661 accatatatc cgtaacagtg cgccacgcag caataaccgc tggagcctgc gcgaaacaca
     3721 cgcaacacca ggcggtcacg ttgtaaaaca aactggggaa cacacctggc tgattgagca
     3781 ggctggactc gtgatccaca gatgcaaccg caatccgttt accggaaacc gcatctttgc
     3841 actgagcagc ggcgacaacc agttcgggca ggatttgacg ctacacgaag ccctgcgaac
     3901 tgccgaccgc ttaatcgcgg ggcgcagctt tcagcacccg gattactaaa gaaaagtcgg
     3961 cttaataagc cctgagccag tcttaataag acatgagagg cgataagatg aaaagagcct
     4021 acaaagaaat cgctaacgac atacttcaga attatcacct aacccttgcg agcaacggtg
     4081 tcgcctatga tgagcaatcg ttccgcgcaa tgcagggtgg gattcttggg ctgtatatcg
     4141 ccgccacagc cagcggcgac ctcgaagcgc tggcagaatt aaaggttatc tatccgaaag
     4201 aacttggcga agacctgatt aaaccctatc tcattgagga gct
//
LOCUS       DBBGLQ010000220         4214 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.220, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000220 DBBGLQ010000000
VERSION     DBBGLQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.220"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            245..853
                     /locus_tag="ACIYSB_005283"
     CDS             245..853
                     /locus_tag="ACIYSB_005283"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGC5432101.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            951..2342
                     /gene="selA"
                     /locus_tag="ACIYSB_005284"
     CDS             951..2342
                     /gene="selA"
                     /locus_tag="ACIYSB_005284"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGC5432102.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2339..4183
                     /gene="selB"
                     /locus_tag="ACIYSB_005285"
     CDS             2339..4183
                     /gene="selB"
                     /locus_tag="ACIYSB_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGC5432103.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGMDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANVGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          960 a         1063 c         1225 g          966 t
ORIGIN      
        1 gccggttttc cgctcatttc tcatccttgt tttatcgggt tatttgtctg ctgacaggcg
       61 aaaccctaca gatttataag aaagcctgaa gtgagatatc catcagagtg aaataattag
      121 gaaaatattt atatcaccat gataaatatg tgatttattt ggaagtaaca aaccatcaac
      181 attttctgcc ctttttccca tcaccaggcc atactgatat ttttcctcgc acaaaggact
      241 cggcatgaaa ctcgtcggta gctacaccag cccgtttgta cgcaaacttt ctattctgtt
      301 gttagaaaag ggcataactt tcgaatttat taatgaactg ccctataacg cggacaacgg
      361 cgtggcgcaa tttaacccgt taggaaaagt gccggtgctg gtgaccgaag agggcgaatg
      421 ctggtttgat tcgccgatca tcgctgaata tattgaatta atgaatgtcg ctccggcgat
      481 gttgccgcgc gatccgctgg agtcgttgcg ggtgcgcaaa attgaggcac tggcggatgg
      541 cattatggat gccgggctgg tatcggtgcg tgaacaggcg cgtccagcgg cgcagcagtc
      601 tgaagatgaa ttgttacgcc agcgggagaa aatcaaccgc agtctggatg tgctggaagg
      661 atatctggtc gatggcacac tcaaaaccga tacggtcaat ctggcgacta tcgccattgc
      721 ctgtgccgtc ggatatctca atttccgccg cgttgcgcca ggttggtgtg tcgaccgccc
      781 acacttagtc aaactggtcg aaaacctgtt tagccgcgaa agttttgctc gcaccgaacc
      841 gccaaaggct tgatgcgcga tatgtcctcc tgacccatct cacgttacaa tccgtggtta
      901 tgttaaacgc ccttctccgt gtgagagggc cttgatcagc caggtttcct atgacaaccg
      961 aaacgcgttc cctctatagt caacttccgg ctattgatcg cttattgcgc gatagctcct
     1021 tcctttcttt gcgtgatact tatggtcaca cccgcgtggt ggaattgttg cgtcagatgc
     1081 tcgacgaagc gcgagaagtg attcgtggca gccagacgct gcctgcgtgg tgtgaaaact
     1141 gggcgcaaga agtcgatgcc cggttgacga aagaagcgca gagcgcgctg cgtccggtga
     1201 tcaacctgac gggaaccgtg ctgcatacca accttgggcg agctttacag gcggaagccg
     1261 cggtggaagc cgttgcgcag gctatgcgtt cgccagtgac cctcgagtat gatctggacg
     1321 acgccggacg cggacatcgc gatcgggcgc tggcgcagct gctgtgccgt attacggggg
     1381 cggaagatgc ctgtatcgtc aataacaatg cggcggcggt gttattgatg ttggcggcca
     1441 ctgccagcgg aaaagaggtg gtggtatctc gcggcgaact ggtggagatt ggcggcgcgt
     1501 ttcgtattcc cgatgttatg cgtcaggcag gctgcaccct acacgaagta gggaccacca
     1561 accgcacgca cgcgaatgat tatcgtcagg cggtgaatga aaataccgca ctgttgatga
     1621 aagtacatac cagtaactac agcattcagg ggttcaccaa agcgatagat gaagcggaac
     1681 tggtggcgct cggcaaagag ctggatgttc ccgtagtgac tgatttaggc agtggctcgc
     1741 tggtcgatct tagccagtac ggtttgccga aagagccaat gccgcaggag ttgattgcgg
     1801 cgggcgtcag tctggtgagt ttctccggcg acaagttgtt aggcgggccg caggcaggaa
     1861 ttattgttgg taaaaaagag atgatcgccc gcctgcaaag ccacccgctg aagcgtgcat
     1921 tacgcgcgga taaaatgacc ctcgcggcgc tggaagccac gttgcgtctt tatttacacc
     1981 ctgaagctct gagtgaaaaa ttaccgaccc tgcgcctgct tacccgcagc gcagaggtca
     2041 ttcaaatcca ggcacaacgt ttacaggccc cccttgccgc acattacggc gcggagtttg
     2101 cggtacaggt tatgccatgt ctttcgcaga ttggcagtgg ttcgctgccg gttgatcgcc
     2161 tgccgagcgc ggcattaacg tttacacccc atgatggacg cggtagccac cttgagtcat
     2221 tagccgcccg ctggcgtgaa ttgccagtgc cggtgattgg tcgtatttat gacggacgat
     2281 tgtggctgga tttacgctgc cttgaagatg agcaacggtt tttggagatg ttgttgaaat
     2341 gattattgcg actgccggac acgttgacca cggcaaaaca accttattgc aggcgattac
     2401 tggcgtaaat gctgaccgtc tgccggaaga aaaaaagcgc ggcatgacca tcgatctcgg
     2461 ctatgcctac tggccgcagc cggatggtcg cgtgcctggt tttatcgacg ttcccggtca
     2521 tgaaaagttt ctttccaaca tgctggcggg cgttggtggt atcgatcacg cgctgttggt
     2581 ggtggcgtgc gatgacggcg tgatggcaca gacccgtgag catctggcga ttttgcagct
     2641 gaccggtaac ccgatgctga cagtggcgct gaccaaagcc gatcgcgtgg acgaagcgcg
     2701 tgttgatgag gttgaacgcc aggtaaagga ggttctgcgg gaatacggtt ttgctgaggc
     2761 aaaactgttt atcaccgcag caaccgaagg tcggggaatg gatgccctgc gcgagcatct
     2821 gcttcagttg ccggaacgcg agcacgccag ccaacatagt ttccgcctcg cgattgaccg
     2881 cgcatttacc gtaaaaggtg ccgggctggt cgtcaccggt acggcgttaa gcggggaagt
     2941 gaaggtaggc gattcactct ggctgactgg tgtaaataaa ccgatgcgtg tacgtgcgct
     3001 gcatgcgcaa aaccagccaa cagaaaccgc caatgtcggg cagcgtatcg cgcttaacat
     3061 cgcgggtgat gcggaaaaag agcagattaa ccgtggcgac tggctgcttg ccgatgtgcc
     3121 gccagagccg ttcacacggg tgattgtcga gcttcaaacc catacaccgc tgacccagtg
     3181 gcagccgctg catattcacc acgccgccag ccacgtcacg ggacgcgttt cactgctgga
     3241 agataacctt gctgaactgg tcttcgacac cccgttatgg ctggcagata acgaccgcct
     3301 ggtattgcgc gatatctctg cccgcaacac gctggccgga gcgcgcgtcg tgatgcttaa
     3361 cccgccgcgt cgcggtaaac gtaagccgga atatctgcaa tggctggcgt ctcttgcacg
     3421 ggcgcagagc gatgccgatg cgttatctgt tcatctggaa cgcggcgcgg ttaaccttgc
     3481 ggatttcgcc tgggcgcgcc agctcaacgg cgaagggatg cgcgaattgc tgcaacagcc
     3541 tggttatatt caggctggtt atagcttgtt gaatgcgccg gttgccgccc gctggcagcg
     3601 gaaaattctc gacacattag cgacttatca tgagcaacat cgcgatgaac ctggccctgg
     3661 gcgcgaacgt ctgcgacgta tggcgttgcc aatggaagat gaagcgctgg tactgttgct
     3721 gattgaaaag atgcgcgaaa gcggcgacat ccacagccat cacggctggc tgcatctgcc
     3781 agatcacaaa gcgggcttca gcgaagagca gcaggccatc tggcaaaaag cagagccact
     3841 gtttggtgac gaaccgtggt gggtgcgtga cctggcaaaa gagacgggaa ccgacgagca
     3901 ggcaatgcgc ctgactctac gccaggcggc gcagcaagga ataattaccg cgatcgttaa
     3961 agatcgttat taccgtaacg atcggattgt cgagtttgcc aatatgatcc gcgatctcga
     4021 tcaggagtgt ggttcaacct gcgcggcgga tttccgcgat cgcttaggcg taggccgaaa
     4081 gctggcaatt cagattctgg aatattttga ccgcattggc tttacgcgtc gtcgtggaaa
     4141 tgatcattta ttgcgcgacg cattattatt tccggaaaaa taaggaaatg attaattaag
     4201 ttttaaaata aatt
//
LOCUS       DBBGLQ010000221         4185 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.221, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000221 DBBGLQ010000000
VERSION     DBBGLQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.221"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..119
                     /locus_tag="ACIYSB_005286"
     CDS             <1..119
                     /locus_tag="ACIYSB_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005974166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron(III) transporter permease"
                     /protein_id="HGC5432104.1"
                     /translation="EAARGTFEEGAISALMIVLAGTFPVILFVPAERLKSED"
     gene            122..788
                     /locus_tag="ACIYSB_005287"
                     /pseudo
     CDS             122..788
                     /locus_tag="ACIYSB_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014700592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1407..1914
                     /locus_tag="ACIYSB_005288"
                     /pseudo
     CDS             1407..1914
                     /locus_tag="ACIYSB_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(2045..2460)
                     /gene="tnpA"
                     /locus_tag="ACIYSB_005289"
                     /pseudo
     CDS             complement(2045..2460)
                     /gene="tnpA"
                     /locus_tag="ACIYSB_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            complement(2492..2668)
                     /locus_tag="ACIYSB_005290"
     CDS             complement(2492..2668)
                     /locus_tag="ACIYSB_005290"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGC5432105.1"
                     /translation="MAGGVRYDLKDHPCGKVTQEHLANAGKRLDEIKKKQQERRKSNR
                     LIKTSFASLSCFLC"
     gene            complement(2744..3064)
                     /locus_tag="ACIYSB_005291"
     CDS             complement(2744..3064)
                     /locus_tag="ACIYSB_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGC5432106.1"
                     /translation="MNNSTLKSRTISDATAEELESRGLWRRAARRWQDVMITLEKDSH
                     RQLAVMRARECIRKAKRPIPESRIDIHTVRKAADRTIQKMGLPSLTDEIWRDYPDSVN
                     DDGY"
     gene            complement(3082..3378)
                     /locus_tag="ACIYSB_005292"
     CDS             complement(3082..3378)
                     /locus_tag="ACIYSB_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGC5432107.1"
                     /translation="MFDDGQATMWLSVLAFGRQAEALARHQKGELVSVAGNMQLSQWT
                     GQNGKTRQGRQVIADSVISTQTVRPGGSRRETTGTQGNQPPTGGDATYGDDIPL"
     gene            3515..4116
                     /locus_tag="ACIYSB_005293"
                     /pseudo
     CDS             3515..4116
                     /locus_tag="ACIYSB_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224844.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1069 a         1000 c         1022 g         1094 t
ORIGIN      
        1 ctgaagcagc ccgtggaact tttgaagaag gggccatctc tgctttaatg attgttcttg
       61 cagggacatt tccggttatt ttgtttgttc cagctgaaag actgaaatca gaggattgat
      121 tatgtctgaa gaaacagctt tatgtattaa tgatgtccat gttgtttatc ccaatagccc
      181 atatcctgta ttgcggggcg gggggtatgt cagtctgtaa aggtgaactg gcatgtctgc
      241 ttggtacatc aggttgtgga aaaacaacag tactacgtgc tgtagctggt tttgagcgcc
      301 tgaacaaggg gaaatttttg tatcacagca ttgtgtggcc agcgcagata tgcattttcc
      361 gccggagaaa tgtcatgtcg gtatagtctt tcaggaatat gcgctctttc cccaccttac
      421 agtttcacaa aacgtggctt tggtctgaga agactgccga gagatgtaca gcgaaccaga
      481 gttacagaac tgttgaaaat ggtggattta ttccactatt cagacagcta tcctcatgaa
      541 ctttcaggag gtcagcagca acgtattgcc cttgcaaggt cactggttcc aaatccagat
      601 atcctgttgc tagatgaacc attttccagc ttggataaaa aacaagggta ggactgagcc
      661 gtgaagttcg tgatattttg catgaggcag ggcagacagc acttttagtc acttacagtg
      721 aacacgaagc atatttgatg ggggctcgta ttagatatgt taaaaacgga caatattggg
      781 aagggtaacc ttgccagcag actgtcaaga tgtattaaac aggtcgatta gctgtaggcg
      841 ggatagctcg cgggataaat catctgagaa tgtgccattt tccgggctgt tatgggggac
      901 aggcattgct ggttgttggt tcgttgatgg agggaaagct gcagcacaga taatggggct
      961 ttaggaaaag gaaagacata ataaccacga caaagtgact atcaaagtgg gccaaggaca
     1021 tttgaatgtt tgagataata aatattgtaa tgaagctgag gctattgcta ccaccacaca
     1081 ctacagaacc tgcaccattg gatgccaaga acaactgtcc gaatacatag aaatgctcag
     1141 ataaaagtgt ttatgtctca tccggcatga aaacgatgag cacattatct acaatccaca
     1201 ggccctccta ttttcccgct tatacattcc ccatcttcca tttttcctca cccgcgccgg
     1261 caccacactg tcatctttct gaggttttac tgtcttacac atgtaataaa aagttatttt
     1321 tctgcaattc gggcatgata tgtaatcccg cgagcataaa acgagtgaat ttgcacatca
     1381 ggtacaacaa gcaagagaga taaacgatga aaccgttaca ttatacagct tcatcactgg
     1441 tgctcggact ggcgttaatg ggcaatgcac aggcagtgac gaccattccg ttttggaact
     1501 ctatggaagg ggaaatgggc aaagaggtgg atacaactgg aaaactttag cgccaggaac
     1561 ggtctgccgt ttgccagcaa aaacaacggt tttgacgcca cggacgcggt gctggagttc
     1621 aacaagccgg agcaggtgaa acacatcgtc atgctcgagg agatgaacaa gaagggcgac
     1681 ttcatctacg tcgggcgtaa ggatgaatcc accgagaagt tctatagagg tgattgcgcg
     1741 atgaccaccg cctcttccgg ttctcttgcc aacattcgcg attacgccaa attcaactac
     1801 ggcgtaggca tgatgcctta cgacgctgac gcgaaagatg caccacaaaa cgccattatc
     1861 ggcggagcca gcctgtgggt gatgcagggc aaagataaag aaacgtatac cggcgcaggc
     1921 gaaaagctgg cggtagggga atcatcggat ctttctgctc atccggcgtt atctccatag
     1981 gtaataattc aacgcctgcg tcagaagtgg ttgttaccgg aagacgccgc gaaacacgcc
     2041 cttcgttttg ccagagtctg agccatttga aaataacatt atcattgatg ccagactctc
     2101 gtgcaatctg ggccacacag gctccgggta aaagagtttg ctgaacaagt ctccattttt
     2161 actctggtga aaatcggttc cgacgggttt tctctgtttt tacaggctga gtcactgtcg
     2221 gggctgaata ttatcgcccc gggaaaagag cccggtcagt tgcacgcagg aatattcacc
     2281 agaaagatgc ccgtcagtga atacctgatg aaatcgcctc accagagaaa aaatagcggt
     2341 tttgctgaca ccggtctgtg cagaaatgcg cgggtacgac agtgtctcgt caaatttcag
     2401 ttgtagcaaa cgcaggatca attccggatt atctgccagt accgcctttc gtgcattcat
     2461 cgtaaatgtc cttttactgc gaaaatggac attagcatag gaaacaggaa agggaggcga
     2521 aagacgtttt aatgagacgg ttactttttc tgcgctcctg ctgttttttc ttaatctcat
     2581 ccagtcgctt ccccgcgttc gccaggtgct cctgtgtcac cttcccgcat ggatggtctt
     2641 tcaggtcgta acgcacgcca ccagccatca gggccggtaa tgttccacca gcggtatata
     2701 ttctcccgag tcacgcccgc tggtatcgtg tttcccccgc cagttaataa ccgtcatcgt
     2761 taacgctatc agggtaatcg cgccatattt catcagtaag cgacggtagc cccatttttt
     2821 gtattgtcct gtctgcggcc tttctgactg tatgaatatc aatacggctt tcaggtatcg
     2881 ggcgttttgc cttcctgata cattcccttg ctctcattac tgcaagctgt ctgtgactgt
     2941 ctttctcaag agtaatcatc acatcctgcc accttctggc agcacgtcgc cacagtcccc
     3001 tgctctcgag ttcttccgct gtcgcgtcgc ttattgtccg gctcttcagt gtgctgttgt
     3061 tcatgttgcg ccccgcttaa ttcataaggg tatatcatct ccgtaagtgg cgtcgcctcc
     3121 cgttggtggc tgattaccct gtgtacctgt ggtttcgcgt ctgcttcctc ctggccgcac
     3181 cgtttgagta ctgattacgc tgtctgcgat aacctgccgt ccctgccgcg ttttcccgtt
     3241 ctgccctgtc cactggctga gctgcatgtt acctgccacg cttaccagtt cgcctttctg
     3301 gtgtctggcc agtgcttcgg cctgtctgcc aaacgccagc acagataacc acattgtcgc
     3361 ctgtccgtca tcgaatatgt tttatcgcca ctttccggaa tatcaattaa cttaattaat
     3421 aacgcgtcca tttcctttgc gcagattaat gcattttcag agtggattaa tacttcaaaa
     3481 gggattttat tcatggctca cccctcacga atacgctgtt tcttatacat aaatccccac
     3541 ccggggtgaa atcatacacc tcgtgattga ttccaacggg ctaaaagtct ttggggaagg
     3601 cgaaaggaaa gccaaaaagc acggcaaaga ggggcgtcgc atctgtctgt tgatggcaac
     3661 acacatgaaa ttgtctgtgc agacctgtcg ctgaacaacg tcacggactc agaagccttc
     3721 ccggggcttc tccaacagac tcacagaaaa atcatggctg cagcggtaga cggggcttac
     3781 gacccccgac tctgtcacga tgaactgagg cgtaagaaaa tcagcgcgct taccccttcc
     3841 cgaaaaggtg cgggttacta gcccggtgaa tacgaagacc gtaaccgtgc agtggctaat
     3901 cagcggctga ccggaagtaa tgcccggtgt aaatggacaa cagattataa ccgtcgttcg
     3961 atagcggaaa cagcgatgta ccgggtaaaa cagttgttcg gaggttcact gacgctgcgt
     4021 gactacgatg gtcaggttgc agagggtatg gtcatggcgc tagcgctgaa taaaatcacg
     4081 aaagtaggta tgcctgagag cgtgcgtatt gcctgaaagc acaactagct acggggctct
     4141 taccctaaat cagatttttt caacaaagcc caactggtat tagaa
//
LOCUS       DBBGLQ010000222         4135 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.222, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000222 DBBGLQ010000000
VERSION     DBBGLQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.222"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..2684)
                     /locus_tag="ACIYSB_005294"
     CDS             complement(<1..2684)
                     /locus_tag="ACIYSB_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001746330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HGC5432108.1"
                     /translation="MNKVYSLRYSAVARGFIAVSEFARKCVHKSVRGLSFPVLLLSPV
                     LFSTGGLAGTVNNELGYQSFRDFAENKGMFRPGATNIAIYNKQGELVGTLEKAAMPDF
                     SSVNSGSGVATLINPQYIASVKHNGGYTNVSFGYGESRYNIVDRNNAPSLDFHAPRLD
                     KLVTEVAPAAVTAQGAVAGAYLDKERYPVFYRLGSGTQYIKDSNGQLTQMGGAYSWLT
                     GGTVGSLSSYQNGEMISTSSGLVFDYKLNGAMPIYGEAGDSGSPLFAFDTVQNTWVLV
                     GVLSAGNGAGGRGNNWAVIPLDFIRQKFNEDNDAPVTFRTSEGGALEWGFNSSTGAGT
                     LTQGTTTYAMHGQQGNDLNAGKNLIFLGQNGQIDLKDNVAQGAGSLTFHNDYTVATSN
                     NSTWTGAGIIVDKGVFVNWQVNGVKGDNLHKIGEGTLTIQGTGINEGGLKAGDGTVVL
                     KQQADSNGQVQAFSSVNIASGRPTVVLTDDRQVNPDNISWGYRGGILDVNGNDIGFHE
                     IKAADYGAQIHNGSNKNAIISLVMHDAIKWTGKDTPRILGRVYEYFNDETQKTEFFIL
                     KTSSIAWSPSPGEKFFPDMFRGADYFNTLEDADKEARKNTQYIFHGKLTGNISFNPKS
                     AGTFVMDGSADMSGTFTRENGRLTLQGHPVIHAYNTQSVADKLAASGDHSVLNQPTSF
                     SQEDWENRSFTFDRLSLKNTDFGLGRNAMLNTTLEATDSTVTLGDSRVFIDKNDGNGT
                     AFTLEEGTSEAVKDADRSVFNGSAVLYGKTTLDIMNATFNGDISGHAGSHVELSRRSH
                     WNMTKSSTLDSFRSKGGTLSFVTDNWSPKTLTVNTLHASSMNIAMGVSTADNTGDRID
                     ILNKATGGHNTLDLSSLFDQTVTLKNDLTLASAPVGTS"
     gene            complement(2996..>4135)
                     /locus_tag="ACIYSB_005295"
     CDS             complement(2996..>4135)
                     /locus_tag="ACIYSB_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HGC5432109.1"
                     /translation="MASELGYVVLIPLSAILFHSLGRHPLAGLAAAFAGVSGGYSANL
                     VLGTIDPLLSGITQQAAQIVSSDYIVGAESNWYFMFVSTFFITLMGYLITEKIVEPQL
                     GKYKGGGFNQDEQSIRNSSDVTAVEKKALMLSFLSVLLLSVIVAILVIPEDGILRNQQ
                     TGLISNSPFLKSIVAFIFIFFSLAGVVYGYTAGTIKNSKDIVDAMTHSMSTLGLYLVI
                     IFFASQFVALFSWTNIGQVIAVKGGSFLNSLDLPDSVIFIGFILICAVINLMIGSASA
                     QWAVTAPIFVPMLMMAGYAPEVIQAAYRIGDSVTNIITPMMSYFGLVMAIVLKYKKDA
                     GVGTLISMMLPYSIVFLVGWSVFFVIWVFVLNLPVGPGASTYYLP"
BASE COUNT         1169 a         1019 c          816 g         1131 t
ORIGIN      
        1 tgagaagttc cgaccggtgc agatgccaga gtcaggtcat ttttcagtgt gacagtctga
       61 tcaaacaggg aggacaggtc cagtgtgtta tgtccccccg ttgccttatt caggatatca
      121 atccggtcgc ccgtgttgtc ggcggtgctg acccccatgg cgatattcat actgctggca
      181 tgcagtgtgt ttaccgtcag cgttttcggc gaccagttgt ctgtcacaaa ggagagcgtc
      241 ccgcctttgc ttctgaacga atccagcgtg gagcttttcg tcatattcca gtgactccgg
      301 cgcgacaact ccacatgcga gccggcatga ccgctgatat caccattaaa ggttgcgttc
      361 atgatatcca gcgtcgtctt cccgtacaga acagcactgc cattaaacac gctacggtct
      421 gcatccttca ccgcttctga tgtgccttct tccagcgtaa aagccgtacc gttaccgtcg
      481 tttttgtcaa taaagacccg gctgtcgccc agtgtgacag tcgaatctgt tgcttcaagc
      541 gtggtgttca gcatggcatt acggccaagg ccaaaatcgg tgttcttcag tgacagtctg
      601 tcaaaggtaa aactgcgatt ctcccagtcc tcctgactga atgacgtggg ctgattcaaa
      661 accgaatggt ctccactggc agcaagtttg tcagccacag actgtgtatt gtatgcatgg
      721 ataaccggat gaccctgcag tgtcagacgc ccgttttccc gggtgaaggt tccggacata
      781 tcagcagaac catccataac aaacgtaccg gcactcttcg ggttaaaact gatattaccg
      841 gttaatttac catggaaaat atattgtgta ttttttctgg cctctttgtc cgcatcctca
      901 agagtattaa aataatcagc acctctgaac atatccggaa aaaatttttc tccaggcgaa
      961 ggagaccacg caatactgct ggtttttaat ataaagaatt cagtcttctg cgtttcatca
     1021 ttgaaatact cataaacccg acctaaaatt cttggtgtat ctttcccggt ccacttaatg
     1081 gcatcatgca taaccagaga gattattgca tttttgttac tgccattgtg gatttgagca
     1141 ccataatctg ccgcctttat ttcgtgaaag ccaatatcat taccgttaac atccagaata
     1201 cctcccctgt atccccagga gatgttatcc ggatttacct gccggtcatc agtcagcact
     1261 actgtcggtc gtccgctggc aatattcaca ctactgaagg cctgcacctg cccgttactg
     1321 tctgcctgct gtttcagaac aacggttccg tccccggcct tcagaccacc ctcattgata
     1381 cccgtgccct gtatcgtcag cgtaccttca ccaatcttat gcaggttatc gcctttaaca
     1441 ccgttaacct gccagtttac gaacacgcct ttatccacaa taataccggc accggtccag
     1501 gtactgttgt ttgaagttgc aaccgtataa tcattatgga atgtcagaga acctgccccc
     1561 tgtgcaacat tgtctttcag gtcaatctgt ccattctgtc ccagaaatat cagattttta
     1621 ccggcattca ggtcatttcc ctgctgcccg tgcatggcat atgtggtggt tccctgtgtc
     1681 agcgtgccag ctccggtgct gctgttaaag ccccactcca gcgcaccacc ttccgatgtt
     1741 ctgaatgtga ccggggcatc attgtcctca ttaaattttt gtctgataaa atccagtgga
     1801 ataacagccc agttatttcc cctgcccccc gctccattcc ccgcagagag aacaccaaca
     1861 agaacccatg tattctgaac agtatcaaaa gcaaataaag gcgaaccact gtcaccggcc
     1921 tcgccataaa tgggcattgc accattaagt ttgtaatcaa aaaccagacc tgaactggtg
     1981 ctaatcattt ctccattctg ataggatgac agactaccga cagtcccgcc agtcagccag
     2041 gaatatgctc ctcccatttg tgtcagctgt ccgttactgt ccttaatata ctgggttccg
     2101 gagccaagac gataaaaaac aggataacgt tccttatcca gatatgcgcc agccactgcc
     2161 ccttgcgccg tcaccgcagc cggggcaacc tctgtcacca gtttatccag tctgggagca
     2221 tgaaaatcca gtgacggcgc attattccgg tccacgatat tgtaacggct ttcaccataa
     2281 ccaaagctga cgtttgtata tcccccgtta tgtttcacgc tggcgatata ctgcgggtta
     2341 attagtgtcg ccacaccgct tcctgaattt acggaactga aatccggcat agccgctttt
     2401 tccagcgtgc cgacaagctc tccctgctta ttataaatgg cgatattcgt tgctcccggg
     2461 cggaacatac ctttattctc agcaaaatca cgaaatgact gatagccaag ttcattattg
     2521 actgttcccg caaggcctcc tgtagagaaa agtacggggg acagtaataa aaccggaaaa
     2581 ctcaggcctc tgacagactt atgaacacat ttcctcgcaa actcagatac agcaataaag
     2641 ccccgggcca cagcgctgta gcgaagggaa taaactttat tcatttttaa tttcccctga
     2701 aaatataatc accaaaataa cagaaagtcg gaagcgtaaa cttacctcag catacagatg
     2761 ttaagtcggc attgagccgg agtcatacaa ttgattttct atttaattac attgtgattg
     2821 caatcttcaa tgtaatcaac tatatattta tttgtgttgt gtaaattatt gtgattttta
     2881 catagaaaca tcctgacact taaaatgcta aagtttttat ggctgcatgg gtttaatatt
     2941 gattcaacct ggaaatggag aataatattc tccattatga tatgtatgat tgactttatg
     3001 ggagataata ggtcgatgcc ccggggccaa cgggtaagtt cagtacaaag acccatataa
     3061 caaagaatac agaccacccg acgaggaaaa ctatggagta tggaagcatc attgatatca
     3121 aagtcccaac accggcatcc tttttgtatt taagtacaat agccataaca agcccaaaat
     3181 aactcatcat tggagtaata atgtttgtta ctgaatctcc aattctataa gcagcctgta
     3241 ttacttcagg ggcatatcct gccatcatta acataggaac aaaaattggt gctgttactg
     3301 cccattgagc agatgcggac ccaatcatta aattaatgac agcacaaatc aatataaatc
     3361 caataaatat tacggaatct ggcaaatcaa gactattcag aaaactcccg cctttaaccg
     3421 caataacctg accaatattt gtccagctaa acaaagctac aaactgagag gcaaaaaata
     3481 taatgaccag ataaagtcct aatgtactca ttgaatgagt catggcatca acaatatctt
     3541 tgctattttt tattgttccc gctgtatatc catatactac accagctaat gaaaagaata
     3601 tgaaaatgaa agccactatt gatttaagaa atggagaatt gctgattaat cctgtttgtt
     3661 gattacgtaa gattccatct tcaggaataa ccagaatggc aacaataaca gatagtagaa
     3721 gtacagataa aaaggacagc attaacgctt ttttctcaac tgctgtaaca tcagatgaat
     3781 tacgaattga ctgttcatcc tgattgaacc ctccaccctt gtatttccct aactgtggtt
     3841 caactatttt ttctgttatc aggtacccca tgagtgttat aaaaaatgta ctgacaaaca
     3901 taaaatacca gttggattca gcccctacaa tataatcaga tgatacaatt tgcgctgctt
     3961 gctgggtgat tcctgacaag agcggatcga ttgtacctaa tactaaattt gctgaatagc
     4021 cacccgagac tcccgcaaaa gcagcagcaa gcccggccaa gggatgtctt cccagagaat
     4081 gaaaaagtat tgccgaaagt ggtattaaaa cgacatagcc aagttctgaa gccat
//
LOCUS       DBBGLQ010000223         4106 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.223, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000223 DBBGLQ010000000
VERSION     DBBGLQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.223"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(107..862)
                     /locus_tag="ACIYSB_005296"
     CDS             complement(107..862)
                     /locus_tag="ACIYSB_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGC5432110.1"
                     /translation="MNRAELTAKAVSLIESATPISRSLAQANEITEAAYHLTRDQKRL
                     LFIVVGRLRYASKDGVLGSGACELTVNEYAEMYNLPSAEASKDIRKAISGLSEKKVTI
                     YNPDESTESEDSYESYPWMIKDAYSPRRGTYIIHLNPYLMPFFTLLDKRFTRLNFTEV
                     SRLTNPYSMRLYESLCQYRKDDGSGFAILGVEWMRERYGLPKSYQRYAEFKRSFLTKA
                     VAEIEKNTKMKIVFSEVTEGGKVTRIKFTYQQS"
     gene            1251..2072
                     /locus_tag="ACIYSB_005297"
     CDS             1251..2072
                     /locus_tag="ACIYSB_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432111.1"
                     /translation="MNIPKFPLPSRPETEIQFHAPTVKDALKYSDLNPAEDEATTTEY
                     LNSMQDGEINDSANWTVQDRRTALWWIFVNSRPDAVMTYSYECSHCGNTHHADINLSD
                     LAQTVEILTVPPYVKTNVPVNGVPTDWILKPLTGKGAELLERMRASLPDMKSPEYSAG
                     VARMRIAELALCTALEDDPEDFTQAANRRFDIIESMALETEFTPLVARIQLMQKDLRH
                     GLKMSIERGSSRLILPPQHCKNAKEGADVTTTLYVPFLNREFIPSIRSEWMANHY"
     gene            2087..>4106
                     /locus_tag="ACIYSB_005298"
     CDS             2087..>4106
                     /locus_tag="ACIYSB_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HGC5432112.1"
                     /translation="MDISPLATLNNYRNGEHSICQKPWKKNTKLKPGNVDRAGKREVI
                     LKENKDRIAIIDAIQGASTNELKALAEIKNAILSDRGSITQLGENQGKVSQRLSRRRI
                     ASEPYVSGDAKNTQANPYIKRLPMKKGRTQVNNGEERLIVATNTSERVHFASSRQSAQ
                     KLKGSVPVAANSAPLEPQSPQSQKGPLRDSNGRFVSQKNNEDIARKKEWQNARKADAK
                     LQEGFLRKLGSIIGVDGNQSSSEESLTDAAGVGAGGPLWMAARGMYDITKEITGKAES
                     LKEWVEKGKGEALSSKATSPAITYPAVVNTQKATSATAFNNAVETKSAQAVEEQTKIL
                     QTNDNKIIDGLENVSDEVVKLRKSVSSGNKFGLSDLWRNRASRRNKINIGDQAGKNKR
                     NKPKRKGRNLGKKALSVGEKAAAGTAAAGAGAGAAKTVKTKVSKAKDISTTSDASKGI
                     AKETKNTAKAVKPAGVVAEEATIKTGETVAKKKTESVALKSAAKIGVKSAASTAARAI
                     PIIGSLAMAGYDAIDGYTDTDAQKAAFGLSDDDAVSEQQKTAYATANVLDMGGLVSGA
                     TNLIGKGISALGFERAGEKLQNFDTGDIARGVNGAVDITKSVFGNLKDTFLSTDENTK
                     QVKKAVEDGTKKTVDAIHSLGEQLQGGRNGEDGVGEHGYTSPTEFSAPA"
BASE COUNT         1292 a          933 c          914 g          967 t
ORIGIN      
        1 caacttgcta ataacaagat acatgcaatt aagatgtaaa aattgccctc aattgccgat
       61 cgttaagggc aaattctaca ccttgagagg ggtgtaaaaa ttgcccttaa gactgctgat
      121 aggtaaattt tatcctggta actttgccgc cttccgtcac ctctgagaag actattttca
      181 tttttgtatt tttttcaatc tctgctacag ccttcgttaa aaaacttctc ttaaattcgg
      241 cataccgctg atagcttttt ggtaacccat aacgctcccg catccattcg acgccaagta
      301 tggcaaagcc gcttccatca tccttcctgt actggcataa ggactcataa agccgcatag
      361 aataaggatt tgtaaggcga gatacttcgg taaaattcag ccttgtgaat ctcttatcaa
      421 gcagagtaaa aaacggcatg agatatggat taagatgaat aatgtaagtc ccgcgccgtg
      481 gtgaataggc atccttaatc atccaaggat aagactcata actgtcttct gattcggtcg
      541 attcatcagg gttatatatc gtaactttct tctcgctaag acctgaaatg gccttacgaa
      601 tatccttgct ggcttcagca gagggcaaat tatacatctc tgcatattcg ttgaccgtca
      661 actcacaggc acctgaacca agaacgccat ccttagaagc atagcgaagt cttcccacca
      721 caataaacaa cagtcgcttt tggtcgcgag tcaggtgata agcagcctcc gtgatctcat
      781 tggcttgtgc caaactacga ctaataggtg tcgctgactc aatcaggctg acggcttttg
      841 ctgttaattc cgctctgttc ataaccctgt gtgatccttc aaatggtgag ggcaattttt
      901 acatcacttt gagggcaatt actacacctt ttgagggcaa atcttacacc ttctgagggc
      961 aaatcttaca ccgatcgtat ctaattgagg gcaaatctta caccagactc ctctacagcc
     1021 cgcgccactg ctggctccag cgacgcctaa aaaggatcta aaaaggatta aaaatggaaa
     1081 aaggtttata aaaatactct gtggatatgt gagtagcgtc acttaaggtg taaaaatcac
     1141 cctcaataat cgcttctcga ccggcaaaga tcttcattca atccccatcg caaatcacac
     1201 actccctatc ctctggaaaa tgaacagata cctgcttttc ggaggaataa ttgaacattc
     1261 caaaatttcc attgccttcc cgtcctgaga cagaaattca gttccacgcc cctaccgtga
     1321 aggatgcgct gaaatattct gacctgaacc cggcagaaga tgaggcaact actacagagt
     1381 accttaactc tatgcaggat ggtgaaatta acgacagtgc taactggaca gttcaggatc
     1441 gcagaactgc tctttggtgg atatttgtta attcgcgccc cgatgcagta atgacctact
     1501 cctatgagtg cagtcactgc ggtaatacgc atcatgcaga tattaacctg agcgacctag
     1561 cccaaacagt agaaatactc actgtacctc cttacgtgaa aaccaacgta ccagtaaatg
     1621 gagtaccaac tgactggata cttaaaccgt taaccggaaa aggcgcggaa ctccttgaac
     1681 gaatgcgagc gtcgcttcct gatatgaaaa gccccgaata cagtgctggc gtggcacgga
     1741 tgcgaattgc tgaactcgct ttatgcacgg cgcttgaaga cgatccggag gacttcacgc
     1801 aggccgctaa ccgacgcttt gacatcattg aaagcatggc gcttgaaacc gaatttactc
     1861 cgcttgtggc tcgcatacaa cttatgcaaa aagacctacg ccacggcctg aaaatgtcta
     1921 ttgagagagg ctcaagccga ctgatcctgc ctccgcaaca ttgcaaaaac gctaaggagg
     1981 gtgcagatgt gacaaccaca ctgtacgtcc cctttctcaa tagagagttt atcccatcaa
     2041 ttagatctga atggatggct aaccattatt aacaacctga ctctatatgg atatcagccc
     2101 gttagcgacg ttgaacaatt accgcaatgg cgagcactcc atatgtcaaa agccctggaa
     2161 gaaaaataca aagctcaagc cgggaaacgt tgatcgcgct ggcaaaagag aggttatttt
     2221 gaaagagaat aaagaccgta tcgcaataat tgacgctata cagggggcga gcacaaacga
     2281 acttaaggct cttgctgaga tcaaaaacgc cattctctct gatagagggt ctataactca
     2341 acttggtgaa aatcaaggta aagtaagtca aagactatcc cgtagacgca tagcaagcga
     2401 accctatgtc tctggtgatg ccaagaatac ccaagctaac ccatatataa aacgccttcc
     2461 gatgaaaaaa gggaggacgc aggtcaacaa cggagaagaa cgtctcattg ttgcaaccaa
     2521 cacatcagag cgcgttcatt ttgcgtcatc acgccagtcc gcccaaaagt tgaaaggttc
     2581 tgtgccagta gccgctaatt ctgctcctct tgaaccacaa tccccccaaa gccaaaaagg
     2641 accgcttcgg gatagtaatg gacgctttgt atcccagaaa aacaacgaag atatagccag
     2701 aaaaaaagaa tggcagaacg cacgtaaggc cgacgccaaa ctacaagaag gcttcctccg
     2761 gaaattaggc tccattatag gggtagatgg caatcagtct tccagtgaag agtctttaac
     2821 agatgccgca ggtgtcggcg ctggtggtcc gttgtggatg gctgctcgcg gcatgtacga
     2881 cattactaaa gagatcacag gtaaagcaga atctcttaaa gaatgggtag aaaaggggaa
     2941 aggtgaggct ttatcttcga aagcgacatc accagctatc acttatccgg cggtggtgaa
     3001 tactcaaaaa gcaacctctg caacagcatt taataatgct gttgagacaa aatcagcaca
     3061 agcagtagaa gagcaaacaa aaattcttca gaccaatgac aacaaaataa ttgatggtct
     3121 ggaaaatgtc tccgacgaag tagttaagct acgcaaatca gtatcttctg ggaataagtt
     3181 cggcttaagt gatctctgga gaaatcgagc aagcagaaga aataaaatca atattggcga
     3241 tcaggctggt aaaaataaac ggaacaaacc gaaaaggaaa ggtcgcaatc tggggaaaaa
     3301 agccctttct gttggtgaaa aagccgctgc cggaaccgct gctgctggag ctggtgccgg
     3361 agctgcaaag actgttaaaa caaaggttag taaagccaaa gacatcagca ccacaagcga
     3421 cgcatctaag ggtattgcta aagaaacaaa aaacacagca aaggcagtta agccagcagg
     3481 tgttgttgcc gaagaggcca caattaaaac aggtgaaact gttgcaaaga aaaaaaccga
     3541 atccgtagcc ctcaaaagtg ctgcaaaaat aggtgttaaa tcagctgctt ctacagctgc
     3601 aagagctatc ccgatcatcg gatcgctggc tatggcggga tatgacgcta tagatggtta
     3661 cactgataca gatgcgcaaa aggcagcctt tggattaagt gacgatgatg ctgtatccga
     3721 acagcaaaaa acggcttacg caacagctaa tgtgctcgat atggggggct tggtgtcggg
     3781 cgcaacgaac ttgataggga aaggaatttc tgcgctagga tttgaaagag caggtgaaaa
     3841 acttcagaat tttgataccg gagatattgc tcgtggtgtt aacggcgcgg tagatatcac
     3901 caaatctgtt tttggtaacc ttaaggatac atttttatcc actgacgaaa acacgaaaca
     3961 agtaaaaaaa gctgttgaag acggcacaaa aaaaactgtc gatgcgattc attctcttgg
     4021 tgaacaatta caaggtgggc gtaatggcga ggatggagtt ggtgagcacg ggtatacttc
     4081 tccaactgaa tttagtgcac ctgcca
//
LOCUS       DBBGLQ010000224         4003 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.224, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000224 DBBGLQ010000000
VERSION     DBBGLQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.224"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(67..669)
                     /gene="fimB"
                     /locus_tag="ACIYSB_005299"
     CDS             complement(67..669)
                     /gene="fimB"
                     /locus_tag="ACIYSB_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGC5432113.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2123..2839
                     /gene="nanC"
                     /locus_tag="ACIYSB_005300"
     CDS             2123..2839
                     /gene="nanC"
                     /locus_tag="ACIYSB_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGC5432114.1"
                     /translation="MKKAKILSSVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2859..3965
                     /gene="nanM"
                     /locus_tag="ACIYSB_005301"
     CDS             2859..3965
                     /gene="nanM"
                     /locus_tag="ACIYSB_005301"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGC5432115.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKRWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWYNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVMDSVLISVKDNKVTVQN"
BASE COUNT         1243 a          804 c          828 g         1128 t
ORIGIN      
        1 aatcattttt gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa
       61 ctatctctat aaaacagcgt gacgctgtcg tcctctggcc ctatcccaga tgccgtaaaa
      121 acgccctgca ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata
      181 atcctggata agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatggcg
      241 taacatgtgc ggatgaatct ccagtgacaa cccggcatta ccaccggaag tcgagataat
      301 atggtaaaac tgttgccgag aaagcggatt ccccttacgc gataaaaata cccattcgct
      361 ctcagcatgc gggtacgaag tacggatact caaccagttt tttaaagcct gaacttcttt
      421 gttcaatagc gggtgcgttg ttgaaaagcc tttttttaat cgatggatat agatacactt
      481 tgccttaaga tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg
      541 aataaaacaa agcaaagtca gacaataatt acgtgctgca tgaggcccgg tatttgctgc
      601 tttaaggagt gattcgattt cactatgggt caggaagttc ctttttttgt tatcagcctt
      661 attcttcatc gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata
      721 atccaacatt ttaatcctga ttatgtttat aaaagcgaac gtttgcttaa taactaacgt
      781 aagtggacca gttcttctga gtgaacttaa atggagtagc gatagttact tataataact
      841 ttgccataca aacgccatgc tcttgcatgc tatgtacctt tatatattta taaatctgat
      901 acgaatcgaa taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat
      961 atgttctgta acaagtaaaa tcaacataaa aaattcagca aaatttaata ttcgttcaac
     1021 tagccgtctc acatatgaca ttattagtca actgcatttg ccacaaaatg gctatttcat
     1081 ggaacgcagt aatttcttag tggctattat catgctaatt cttagatccc tagcgattat
     1141 tcctgcctgg ttcgtcagct taggccatga gattgttaca ttccctttta tcctggagat
     1201 atgggggaac gcaggcgacg ctttttcatc tacatccgtt ggtaactatg gctttggtca
     1261 ctacgactta aaccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa
     1321 gtgaaacatc atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt
     1381 gaccagttac aagccacaaa ccaagaaaca tatttcatga tgagaattat actcagcctg
     1441 atggcgggta aacacttatt ttcaccaagc tcactgagtt agcttgcaaa gctttctttg
     1501 tttagattct aaataaatta tttacaaaac agattgttaa aagaaaacct ttagtttttg
     1561 aagaccaatg caagtgttcg tctggcttca catggcatct gcttcttagg aaaagatcga
     1621 catattttgt gacacgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat
     1681 ctggtataac aggtataaag gtgcaccacg atagtcaatg agactgggca tctcgtaatc
     1741 tatggcaaac atcacttcag ttctttctca tcgggtgatg aaaacgcact tcagtctgaa
     1801 aggaatatga aaatgagatc aacagacact ctattttatg actctgggta aaatggattg
     1861 agtaagcgat atagcttacg aaaattcaaa tcaattaaac atcagcagag attttatact
     1921 caggtattta atctggccct ctgtttattt aaataatgtg gaaagagatt tttcacagga
     1981 gatattatac aaaaaatata aaatacagct accggttgcc aaagacacta taagcctggc
     2041 aaaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
     2101 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctagcg tattattact
     2161 gtgcttttcg tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg
     2221 tagtggaagc cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
     2281 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
     2341 cgcactcaat gatgttcagg ttgaagttaa ttacgcgatt aaacttgatg atcaatggac
     2401 ggtgcgcccg ggaatgttaa cgcattttag cagcaatggc acacgctacg gaccctacgt
     2461 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
     2521 gaaagcttac cgacaacaag acttatctgg tgatatgtct cgtgataacg ttcatcgttg
     2581 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
     2641 atacagtaaa cagaacgatt atcgctatgc aaaccataag aaatgggcga cggaaaatgc
     2701 atttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
     2761 tgaccgtcag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
     2821 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaaca ataacggcgc
     2881 ttactatcat aatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc
     2941 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
     3001 caggtacggc atggtacaag ctggatacac aggccaaaga taaaagatgg acagcgttag
     3061 ctgcattccc tggcggaccg agagatcaag caacctctgc atttattgat ggcaatctgt
     3121 atgtgtttgg cggcattggc aaaaacagcg agggcttgac tcaggtattt aatgacgtac
     3181 acaaatacaa ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg ccgatgggca
     3241 tggcgggcca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     3301 agaatatctt caatggctat tttgaagatc tcaacgaagc tggaaaagat tcaaccgcta
     3361 tagataaaat caacgcctac tattttgaca aaaaagcaga agattatttc ttcaataagt
     3421 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tctccctggt
     3481 acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg
     3541 aagccaaacc aggattgcga acggatgccg tatttgaact tgacttcacc ggtgataatt
     3601 taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     3661 ggataagcaa tgattctctt atatttgctg gaggagccgg attcaaaggt tcacgagaaa
     3721 attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcgctgata
     3781 ttcatctttg gtataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct
     3841 acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg
     3901 gcaaagcagt gatggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa
     3961 actaacgctt cagggccccg gtaaaggggc cctgctatca att
//
LOCUS       DBBGLQ010000225         4000 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.225, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000225 DBBGLQ010000000
VERSION     DBBGLQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.225"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            114..1340
                     /locus_tag="ACIYSB_005302"
     CDS             114..1340
                     /locus_tag="ACIYSB_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGC5432116.1"
                     /translation="MSGTIRKIIKEDDVFHSSIRRIEWLFETFSSVCLSFSGGKDSTV
                     LLHLAADVARRKKRRFSVLFIDWEAQYQCTIEHVQKMRGMYRDVTDTFYWVALPLTTV
                     NGVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNR
                     CGVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKARDI
                     WIYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAV
                     YLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMKERRKEWGIL"
     gene            1325..1969
                     /locus_tag="ACIYSB_005303"
     CDS             1325..1969
                     /locus_tag="ACIYSB_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGC5432117.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVACINPERKGQASRIAATIRHNRARGKHQVTS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            2051..2200
                     /locus_tag="ACIYSB_005304"
                     /pseudo
     CDS             2051..2200
                     /locus_tag="ACIYSB_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558245.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(2463..3466)
                     /locus_tag="ACIYSB_005305"
                     /pseudo
     CDS             complement(2463..3466)
                     /locus_tag="ACIYSB_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381461.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            3559..>4000
                     /locus_tag="ACIYSB_005306"
     CDS             3559..>4000
                     /locus_tag="ACIYSB_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5432118.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRIFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTLRNGRSPLHVFVAVPGY"
BASE COUNT          979 a          948 c         1039 g         1034 t
ORIGIN      
        1 tttaagaaga atacaaacta attttaagtc aaaagagttt cattgtcgtt caaagcgttg
       61 atttattata aataaaaata ttaattagtt ctttaatttt tgttatatac aacatgtcag
      121 gaactatacg aaagataata aaagaggatg atgtatttca ttcttctatt cgccgtattg
      181 aatggttgtt tgaaacattc tcttctgtct gtttgtcttt ttccggagga aaagactcca
      241 ctgtgctgct ccatcttgcg gctgatgtgg ctcgcaggaa gaaacgtcgt ttctctgtat
      301 tattcattga ctgggaagct cagtatcagt gcaccattga acatgttcag aagatgcggg
      361 gaatgtaccg ggatgtgacg gatacctttt actgggtggc actccccctg actacggtaa
      421 acggtgtctc tcagtttcag ccggaatgga tatgctggga accaggtgtt gagtgggttc
      481 gtcagccacc agatgacgct attacagata tgtcgtattt cccattttat cggtatgcca
      541 tgacgtttga agaatttgtt ccggcatttt cttcctggtt tgccggtaac cggtgtggag
      601 tggcaatact gactggtgtt cgtgctgatg aatcgctcaa tcgctttatg ggactggtgt
      661 ctcagcgcaa actgagatat gcagatgata aaccctggac cacagcgtca cctgaagggt
      721 tttattacac cttgtatccg ttgtatgact ggaaagcccg tgatatatgg atatataacg
      781 ccagaacccg ggctatctac aatcccctgt atgacctgat gtaccgtgcc ggcgtgccgt
      841 tacgcaacat gcgggtctgt gagccttttg gcccggaaca gcgtaaggga ctgtggcttt
      901 accatgttct ggagccggaa acctgggcca ggatgtgtga gcgggtgtcg ggtgctgcca
      961 gcggggcgct ttatgccaat gagagcggtg cctattttgc cctgcgtaag cgtatcacga
     1021 agccacctca tcatacctgg cgtagctatg cgatgttcct gctggatgtg atgccggaaa
     1081 gaacggcaga acattaccgt aataaaattg ctgtctacct gcgctggtat cagacgcggg
     1141 gcttcccgga tgacatcccg gatgaacagg agaatgacct ggggagccgg gatatcccgt
     1201 cctggcgacg tatctgtaag acactcataa agaatgattt ctggtgtcgg accctctcct
     1261 tcagtccgaa caaaccccgg cactatgaac gttatctgca gcgtatgaaa gaaaggagga
     1321 aggaatgggg gattctgtga cacctgaggt ggaagtattg agcaacatga ttcgacagta
     1381 cttcagtcag gaacgatctg aagaagagac catacgagcc cttaatcatc tgcgcagggt
     1441 gttgcatgag gtcagccctt ttgcacagga gcctgtggac tgtgtgttgt gggtgaaagc
     1501 ggatgaggtg gtggccaacg attacaatcc caatgtcatg tcttccagtg aaaaaaaact
     1561 gcttaaacat tcactggagc aggatggatt cacgcagcct gtggtggttt cggaagagaa
     1621 ggagcattat ctggtcgtgg atggttttca tcgtcagttg ctgggcagga aagcggatac
     1681 gagaaaacgt ctgaagggct ggctgccagt ggcctgtatt aatccagaga gaaagggaca
     1741 ggcatcacgt attgccgcaa ccatacggca taaccgtgcc aggggaaaac accaggtaac
     1801 gtcaatgtct gacattgtca gggacctctc acgtctgggc tggacagatg agcgtattgg
     1861 cacagagttg ggcatggacc aggatgaagt gctgaggctg aaacagatta gcgggctgac
     1921 agagctgttt caggaagagg atttcagccc tgcatggacg gttcgttaag ggctatgttc
     1981 tgacgagagt gtaatttaaa ttttgtaatt gcctgttttt gatatgttcc ctacaccgtg
     2041 gagacaggca attgataaac aagaatccaa tgctattgct gccgaactgg taaattgcct
     2101 taaaaccgaa tctgatctta acctgttttt atacatgctt acgaagtgta ctgttaaaga
     2161 tgagcttagc gctgaactca cagagcatct tggacatgag aacacctgag aataaaggca
     2221 gcaatgcaag taaagactac tcgattaaaa cgctgctcat aaatgatgcc ctaacaattt
     2281 gtgaaatagt atttcgtccg ggtgttcgct ttgtttatgt cgttacgggg aagtcatctt
     2341 cacagggatt tttagtcagt tagcattacc gatatccatt cactaactta ccggagataa
     2401 ttgatgtcaa aaccccaatg gaccattgaa cagtaagcgg ttcgccagaa ccgtattgat
     2461 atttactgag agctcagatc aactttccag ggcaacagat cgcgtacccg gtttgccggc
     2521 cagtcctgga tatgctcaat gacgtaacgc agccattttt ctggttcaac attgttcaga
     2581 cggcatgtgc cgatcagcga gtacaacacc gccgcatgtt cgccaccgct gtcggaaccc
     2641 gcgaacaacc agtttttccg gcctacgtcc actccccgta aggcgttctc tgcgatgttg
     2701 ttgtcgattt ccacccagcc attactgcag tacacgttca ggctatccca ctgtttcagc
     2761 aggtaggcga acgcttttac cgtatccgag tgacgcgaca gtgttttcat ctgggtctgt
     2821 atccagtcat acagtgactg catcagtggc gcagctctgg cttttcttgc cgccagacgc
     2881 tgttctgctg tacatccccg gacttctgcc tcgatggcat acagttcacc gatacgctgc
     2941 agggcttccg tggtgatgtc agttggcgct cttgcatgca aatcgtggat tttcctccgg
     3001 gcatgggcca tacacgcggc ttccgttatt ctgccggatt cgtataacgc ccggtaacca
     3061 ccgtaagtat cggcctgaag cacaccgctg taaccggcca gatgattctg tggatggata
     3121 cctttccggt cctgactgta cgcgaaccag accgccgggg gcatctccga cccggcatta
     3181 cggtcatcac ggacgtagac ccacagcctg tccgttcggt ttttaccact gcctggctcc
     3241 tggaccggaa cggggatatc atcagcatgg actttaccgg gcatcagcac atactggcgc
     3301 aggacgtcat acagcggctc cagcagttca gcaacggtgt cagctccacg tcctgactgc
     3361 ggtatatttc tgactgacgg tataacggca gatggtctgc atacttcccg gtgacaacat
     3421 gggccagaag ccccgctccg gcataactgt gtgcaatggg ttttgaatga gctgtcacga
     3481 acggtgcaat agtgatccac acccaacgcc tgaaatcaga tccagggggt aatctgctct
     3541 cctgattcag gagagcttat ggtcactttt gagacagtta tggaaattaa aatcctgcac
     3601 aagcagggaa tgagtagccg ggcgattgcc agagaactgg ggatctcccg caatacggtt
     3661 aaacgttatt tgcaggcaaa atctgagccg ccaaaatata cgccgcgacc tgctgttgct
     3721 tcactcctgg atgaataccg ggattatatt cgtcaacgca tcgccgatgc tcatccttac
     3781 aaaatcccgg caacggtaat cgctcgcgag atcagagacc agggatatcg tggcggaatg
     3841 accattctca ggatattcat tcgttctctc tcggttcctc aggagcagga gcctgccgtt
     3901 cggttcgaaa ctgaacccgg acgacagatg caggttgact ggggcactct gcgtaatggc
     3961 cgctcaccgc ttcacgtgtt cgttgctgtt cccggataca
//
LOCUS       DBBGLQ010000226         3995 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.226, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000226 DBBGLQ010000000
VERSION     DBBGLQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.226"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            104..1171
                     /locus_tag="ACIYSB_005307"
     CDS             104..1171
                     /locus_tag="ACIYSB_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGC5432119.1"
                     /translation="MSKLVLEQVTKTFGEFYAAREISFCAEEGEFVTLLGPSGCGKTT
                     LLKMIGGFHQPDSGRILIGDKTVNALPPEKRNTAMCFQSYALFPHLNVAHNICFGLKQ
                     KKVAISEQQDRLTEALKQVGLESQRLKMPAQLSGGQQQRVALARAMITRPDVILFDEP
                     LSNLDAKLRESVRFEIKELQRQYQLTSIYVTHDQAEALAMSDKIVVLNAGNVEQIGKP
                     EEIYYRPANRFVADFIGAANIDTAQVTPSEQPGYYRVRCALGEFYVHSVQPPRTEHCY
                     ICWRPEDAQYVSQPQAGNNHFSLTLDKMAFMGNLTEAWGHNDSGLSVRLQLIKKPPVA
                     PGESACFRLAENAIHFLEPVS"
     gene            1168..2034
                     /locus_tag="ACIYSB_005308"
     CDS             1168..2034
                     /locus_tag="ACIYSB_005308"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012304116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5432120.1"
                     /translation="MKNWRNDAVAWLLMLSGLGTILLLMGSTFYVVIVQSLGWFNIEG
                     DSNFSLEYWRNMLQDEVLQSALFYSVKVSLLGAFGSVIFAYPLALWLRKPMAYKEGII
                     AVLRAPMFIPGLVAAFLFVNIVAYHGIINELLLALGIISEPLRMQNDDFGWGVVILQI
                     WKNLPFALILVGGAVNAIRNDVLDAASNLGASRWRSFTGVVVPLTLPAVQVTLILVFI
                     GALGDFAFFSVAGPRNTYSLARLMQATAMEYGEWNNAAVIAVIIMVTAAICTLLIATL
                     ITPFATRKGEVK"
     gene            2031..2867
                     /locus_tag="ACIYSB_005309"
     CDS             2031..2867
                     /locus_tag="ACIYSB_005309"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGC5432121.1"
                     /translation="MSHTANGRRVVTISLIFFVVANLIWLGMPFGMAILWSLVDPAHP
                     WSYPDIFPPVLSFSRWLLVWEKTSLSEALFNSYTIAPAVAVASLLLSLPTAYAFGRMT
                     FRGKAVAEMLTLIPLVMPGMLIGIFFSAMLINLNIDNAFISIVIGHTVLTLPYSIRIM
                     SAGFKSVPQDLIDASRDLGSGAWDTFCNAYLPLLKPSFLAALIFCLVRSLEEFGISYV
                     LGAPDFITVPTILYSFLGYSFVRPDAAVVSMILVIPNVILMVIIEKLLKGNYLSQSTG
                     KA"
     gene            2889..3965
                     /locus_tag="ACIYSB_005310"
     CDS             2889..3965
                     /locus_tag="ACIYSB_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012304117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGC5432122.1"
                     /translation="MKKTVMILAGGLLCSSLATAADSPDLIAQAKKEGSVTFNVWYLQ
                     PQWRSFVKDFEQQYGVKVRIPEGNIDGNVNKLLAEAGKKRGKIDVIALSVAQLPVAMG
                     AKALAKVDDLPGYGDAIHQIQNVDTQGYAVAFWGNQTGFAYDPQQMGDRALPQTLEQL
                     QSFIDANPKKFGYNDPNNGGAGEAFIQRIVTTQGDTFNSEAENVDPAVVKNWQKGWQW
                     FAANKDKITRTASGADSLTRLNDGELMLTPSWEDHLVGLQKTGAINSRLKFYVPEFGM
                     PGGGNIAAIAANSPHPAASRLFLNWLIQPETQHALSKVFGSTPMNKQLRPDLNEIPEP
                     TVKFYGKAYSTQLKKDFVREVMMQ"
BASE COUNT          857 a         1105 c         1154 g          879 t
ORIGIN      
        1 gatatttaga tcatttttta gctttaagga tctgtgtcac ataaatttaa tgtttagtat
       61 gtaaatttaa taatcatctt tttaaccgat ggaagagaga ctcatgtcaa aactggttct
      121 ggaacaggtg accaaaacct tcggcgaatt ctatgccgcc agagaaatct cgttctgcgc
      181 cgaagagggg gagttcgtga ccctgcttgg gccgagcggc tgcggtaaaa ccaccctgct
      241 gaaaatgatc ggcggttttc accagccgga cagcggacgc attcttattg gcgataaaac
      301 ggtcaacgcc ctgccgccag aaaagcgcaa taccgccatg tgtttccagt cctatgcgct
      361 gtttccgcac ctcaacgtgg cacacaacat ctgcttcggc ctgaagcaga aaaaagtcgc
      421 tatcagtgaa cagcaggacc ggctgacgga ggcgctgaag caggtggggc tggagagcca
      481 gcgcctgaaa atgcccgccc agctctccgg cggccagcag cagcgcgtgg cgctggcgcg
      541 ggcgatgatc acccgcccgg acgtgatcct gttcgacgaa cctctctcca accttgatgc
      601 caagctgcgc gaaagcgtgc gttttgaaat caaggaactc cagcggcagt atcagctcac
      661 ctcgatctac gtcacacacg accaggcgga agcgctggcg atgtcggata aaattgtggt
      721 gctgaacgcc ggtaatgttg agcaaatagg caaaccggaa gagatttact accgtccggc
      781 caaccgcttt gtggccgact ttatcggcgc ggccaatatc gacaccgcgc aggtcacgcc
      841 ctcagagcag ccgggctatt accgggtacg ctgtgccctc ggcgagtttt atgtgcattc
      901 cgtacagccg ccacgcacag agcactgcta tatctgctgg cgaccggaag atgcgcagta
      961 tgtcagccag cctcaggcag gcaacaacca cttctccctg acgctcgaca aaatggcctt
     1021 tatgggcaac ctcaccgaag cctggggcca caacgacagc gggttatcgg tccgcttaca
     1081 gctaattaaa aagccgcccg tcgcccccgg cgagagtgcc tgcttccgtc tggcggaaaa
     1141 cgccatccat tttctggagc cggtatcatg aaaaactggc gcaatgatgc ggtcgcctgg
     1201 ctgctgatgc tcagcgggct gggcacgatc ctactactga tgggatcgac gttttatgtg
     1261 gtgatcgtcc agagcctggg ctggttcaac atcgaaggcg acagcaattt ctcgctggaa
     1321 tactggcgca atatgttgca ggacgaagtg ttgcaaagcg ccctgttcta ctcagtgaaa
     1381 gtgtcactgc tgggggcgtt cggctcggtg attttcgcct atccgctggc gctgtggctg
     1441 cgtaaaccga tggcctataa agagggcatt atcgcggtgc tgcgtgcgcc gatgtttatc
     1501 cccggtctgg tggcggcgtt tctgttcgtc aatatcgtcg cttatcacgg cattatcaac
     1561 gaactgctgc tggcgctggg gattatcagc gagccgctgc ggatgcagaa cgatgacttc
     1621 ggctggggcg tggttatcct gcaaatctgg aaaaacctgc ccttcgccct gatcctcgtc
     1681 ggcggcgcag tcaacgccat ccgtaacgac gtgctggacg ccgccagcaa tctcggtgcc
     1741 agccgctggc gcagttttac cggcgtggtg gtgccgctga cgctgcccgc cgtgcaggtg
     1801 acgctgattc tggtatttat cggcgcgctg ggcgacttcg ccttcttctc ggtggccggg
     1861 ccacgcaaca cctactcgct ggcgcggctg atgcaggcca ccgccatgga gtacggcgag
     1921 tggaataacg cggcggtgat tgcggtgatc attatggtca cggcggccat ctgcacgttg
     1981 ctgattgcca cactgattac cccgttcgcc acccgcaaag gagaggtcaa atgagccaca
     2041 ctgccaacgg tcgccgggtg gtgacgattt cgctgatctt cttcgtggtc gctaatctga
     2101 tctggctggg tatgccgttc ggcatggcga tcctctggtc gctggttgac ccggcccatc
     2161 cgtggagcta cccggatatc ttcccgccgg tgctgtcgtt cagccgctgg ctgctggtat
     2221 gggaaaagac ttcgctttcc gaggcgctgt tcaacagcta caccattgcg cctgcggtag
     2281 cggtcgccag cctgctgctg tcgctaccca ccgcgtatgc cttcgggcgc atgacctttc
     2341 gcggtaaggc ggtggcggag atgctgacgc tgatcccgct ggtgatgccg gggatgctga
     2401 tcgggatttt tttcagcgcc atgctgatca acctcaatat cgacaacgcg tttatcagca
     2461 ttgttatcgg ccatacggta ctgacgctac cctattcgat ccgcattatg tctgcggggt
     2521 ttaagtctgt gccgcaggat ctgatcgacg ccagccgcga cctgggctcc ggcgcgtggg
     2581 acaccttctg caatgcctat ctgccgctgt taaaaccgag cttcctggcg gcgctgattt
     2641 tttgtctggt gcgaagcctg gaagagttcg gcatctcata cgtgctgggc gcgccggact
     2701 ttatcaccgt tccgaccatt ctttactctt tccttggcta ctccttcgtc cgcccggatg
     2761 cggcggtggt gtcgatgatt ctggtgatcc ccaatgtcat cctgatggtc atcattgaga
     2821 agttgctgaa ggggaactat ctgtcgcaat ccacgggaaa agcctgacct tttacaggag
     2881 ataatgtgat gaaaaaaaca gtgatgattc tggcgggcgg cctgctgtgc agctcgctgg
     2941 caacggcggc ggatagcccc gacctgattg cccaagcgaa aaaagagggc agcgtgacct
     3001 ttaacgtctg gtacttacag ccgcagtggc gcagctttgt gaaggatttt gagcagcagt
     3061 acggcgttaa ggtgcgcatc ccggaaggga atatcgacgg aaacgtgaac aagttgctgg
     3121 cagaagcggg gaaaaaaagg ggtaaaatag atgttatcgc cttgtctgtt gcgcaactgc
     3181 cagtggcaat gggagcgaaa gcgctggcga aggtggacga tttgccgggc tatggcgacg
     3241 ccattcacca gattcagaac gttgacaccc agggttatgc cgtggcgttc tggggcaacc
     3301 agaccggatt tgcttacgac ccgcagcaga tgggtgaccg ggcgctgcca caaacgctgg
     3361 agcagttgca gagctttatc gacgccaacc cgaaaaagtt cggctataac gacccgaaca
     3421 acggcggcgc gggggaagcc tttatccagc gcatcgtcac tactcagggc gacacgttta
     3481 acagcgaagc ggagaacgtt gacccggcag tggtgaaaaa ctggcagaaa ggctggcagt
     3541 ggtttgctgc taataaggac aaaattaccc gcacggcctc cggtgctgat agtctgacac
     3601 gcctgaatga cggcgagctg atgctgacac catcctggga agaccatctt gtcggactac
     3661 aaaaaaccgg ggctatcaat tcgcggctga aattttacgt accggagttt ggcatgcctg
     3721 gcggcggtaa catcgctgcc attgccgcca acagcccaca tccggcggca tcacgcttat
     3781 tcctcaactg gctgattcag cctgaaacgc aacatgcact gagtaaagtc ttcggctcca
     3841 cgccgatgaa caaacagctc cgtccggatc tgaatgagat cccggagccc acggtgaagt
     3901 tttatggcaa agcgtacagt acacagctca aaaaagattt cgttcgtgag gtcatgatgc
     3961 agtaagcttc atttcgcgca cagccatccc cctga
//
LOCUS       DBBGLQ010000227         3991 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.227, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000227 DBBGLQ010000000
VERSION     DBBGLQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.227"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(39..263)
                     /locus_tag="ACIYSB_005311"
                     /pseudo
     CDS             complement(39..263)
                     /locus_tag="ACIYSB_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(662..871)
                     /locus_tag="ACIYSB_005312"
     CDS             complement(662..871)
                     /locus_tag="ACIYSB_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432123.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWSGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            complement(873..1061)
                     /locus_tag="ACIYSB_005313"
     CDS             complement(873..1061)
                     /locus_tag="ACIYSB_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432124.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAAQNRWRNHLRTKGE"
     gene            complement(1407..1886)
                     /locus_tag="ACIYSB_005314"
     CDS             complement(1407..1886)
                     /locus_tag="ACIYSB_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432125.1"
                     /translation="MTNKIDYQKLREIAEKTKIAGEAPVMPFDQRINALNDFMKHFSP
                     DIALALLDEVKRLEDTNIDAMCRIAEVEAREIKPAKGEVLVVVSGFTGCGKSAIAGEI
                     EIAMKAIGVPVQWTNGDAEKHMTGADWLTAIEMYKPTVRIVEVNVPRAAGIRIKEGE"
     gene            complement(1883..2755)
                     /locus_tag="ACIYSB_005315"
     CDS             complement(1883..2755)
                     /locus_tag="ACIYSB_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HGC5432126.1"
                     /translation="MHQVVSFSGGRTSAYLVHLMEAQRKAGASVHFIFMDTGCEHPLT
                     YRFIREVVKFWNVPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKKYG
                     TPYIGGAFCTDRLKLTPFTKYCDDHFGRGNYITWLGIRADEPRRLKPKPGVRYLAELS
                     DFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRIFNELVTG
                     KHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYRNLYQAMVQAKRFDTGSCSESCEI
                     WGDQLELRFEEVVA"
     gene            complement(2765..3445)
                     /locus_tag="ACIYSB_005316"
     CDS             complement(2765..3445)
                     /locus_tag="ACIYSB_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432127.1"
                     /translation="MKERGMIFNDEMVRAILEGRKTQTRRPVKNVRADNCLVIRKPTK
                     KRNGVYTHVMDAPEHGLCPFGNVGDRIWVREAWAILGNEDGCSVDWNDNLCRGDEKNA
                     ARIYRASCEQKPGDYGLWSIPDDADWKPHTVNEKFDGGWCPSIHMPRWASRILLEITD
                     VRVERLRDMSEADAKAEGASPAMYKITPPEAVYRVGFGDIWRSIYGQDNWLSNPWVWV
                     IEFKRVQE"
     gene            complement(3462..>3991)
                     /locus_tag="ACIYSB_005317"
     CDS             complement(3462..>3991)
                     /locus_tag="ACIYSB_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGC5432128.1"
                     /translation="PPERDLSMVELTFWAIRRNLKDELPDEAGRIAFCQPKTEIPTGT
                     LKESDITWEHSTRELVDITAEQIVNLSVDEDSGLLYMRRPKAVLGKSPAYLRFVVSRP
                     CIGCGGKVNHPFMYRARSLNEHDRWAVPLCDECARSAENDVRAWEKAHGIRLYVAANQ
                     LFDFAIERGVITFNN"
BASE COUNT          950 a         1107 c          865 g         1069 t
ORIGIN      
        1 tctggtcgaa atattcaccg ttaactgcca gttgaaggtt agccacggtt aacctccttc
       61 ggcggttctg gtagcggcat ccagtgggtt acattgcggc tctgcgtttc gaagaactcc
      121 tcaccattgc ggacgacatc aaaaaattca ccgtctcgat attgcgcata aagaacgaat
      181 gcgccatcac ataaaataat tacgtgctga ccatcatctg gcattcgctc actacagctt
      241 atccaaccat ccggagttac cggagagttg ccatttacat cgaagtttgg ctctgcgtcc
      301 tgaaccagga ggatgtaacc attcttggct gtatcaagtt ctaacgcctc ggtgacggtg
      361 ccgaaatagc gattacctaa atcagcatca caagtgctta catcaatgga aacttccatg
      421 ccttcgatta attctggcaa gttgtaagtt tggcttacag gctctgcttc cagcgatgcc
      481 agcacaattc gtgccaactc acgcacaact tcagggggcg cgtgacggtc attcaggtca
      541 tcccacagac gtagcatgtt atcgctacca gggtgaacat cctcattagt tccggccagc
      601 gcactaataa cctcatcggc tgcttcaata attttctgtg cctgttctct ggtaatagtg
      661 gtcatttgtt aatcctcaaa actttatgcc cgggcgcaaa agcacgtgtt ttgtctttac
      721 ttattcgcca cccgtcttta cgggcctctt ttgcacaacc agaccatgac gtaccgatat
      781 actcaccgaa gtctggcact ggatatacac cttccgtaca ctggcggcag tcacaataga
      841 gatgcatggt gtaacttgcg gcaatagcca tatcactctc ctttagtgcg caaatggttt
      901 ctccagcggt tttgcgccgc gctgggcttt ttgcaaaaac cacaatccat catcccgtaa
      961 tatttcatca accccatccg tcggttgctg agtctcaccc actgccagac gccaggagcg
     1021 tttctacgaa ctaacagaat ctttgcttta cggtttttca tcttactgcg taccctttct
     1081 tccgcctgtt ctgtgacgca gtaggcttac gctttgcggc aaaagccacc tgaccaaatg
     1141 gatggagtac cgctatctta tggttgctaa taaccagctc caccacacgc acaggtcgct
     1201 gtaaaaaaag tcgttttgcc ttacggtttt tcatcgcttt gctctcctgc gtctctttgc
     1261 tgctcgtcgt gccgctgcaa taccggtatg gcggcgcttt ggtgccggga tgatgttgtc
     1321 agccatcagg acatgtggct ttgcaattag cgcagaagcc caaaaacgag tcgggtacgg
     1381 taacaagccg atacatgcca cacgcattac tcaccttctt tgatgcgaat gccagcggcg
     1441 cgtggcacat taacttccac gatgcgcaca gttggtttgt acatctcaat cgctgtcagc
     1501 cagtcagcgc cggtcatatg cttttctgca tcgccattag tccattgaac cggcacgcca
     1561 atagctttca tcgcaatttc tatttccccg gcaatggcgc ttttcccgca accagtaaaa
     1621 ccagagacaa cgacaagaac ttcgcctttg gctggtttta tttcccgcgc ctccacctct
     1681 gcaattcggc acatagcatc gatatttgtg tcttccaggc gtttaacttc atccaataat
     1741 gccaacgcga tatctggcga aaagtgcttc ataaaatcgt taagcgcatt aattcgctga
     1801 tcgaaaggca ttacaggcgc ttcaccagca atttttgttt tttcagcaat ttcacgaagc
     1861 ttttgatagt caattttgtt tgtcatgcca ctacctcttc gaatctcaac tccaattggt
     1921 caccccagat ttcacatgat tcggaacacg aaccagtatc aaaccgcttg gcctgcacca
     1981 tcgcctgata caaatttctg tagtcgctgt cggcatacat tctggcaatc ccgtcaagcg
     2041 tcaggtgacc acggtacata acgtctttac ctgttctgcg atgaccatcc ctgacgtgtt
     2101 tgcctgtaac cagctcatta aaaattcgca tcagaccagg ttcgtcttta catgcaagcc
     2161 ccagcttttg cgttgacttt ttgatgcaaa aaacacagtt cccgaggtgc tccgggattt
     2221 gcaaatcaaa aggttgtttt cgccaccacc ggataacatc cgacttatca aaatctgaca
     2281 gctcggcaag ataccggacg cccggtttcg gtttcagcct acggggttcg tccgcacgaa
     2341 tacccagcca tgtgatgtaa ttccctcgcc caaaatggtc atcgcaatat ttcgtgaaag
     2401 gggtgagttt tagcctgtca gtacagaacg cgccgccgat gtatggcgta ccgtactttt
     2461 taaccatgtc cataaacggt ttaagcaccg gcattcgcgt ctgaatatcc tttggttccc
     2521 attccgtata accatttggc tgcccaagtt ctggatttat atcgacctgc aacaccgtta
     2581 gcggtacatt ccagaacttc acaacctcac gaataaagcg atacgtcaga ggatgttcac
     2641 agccggtatc cataaaaatg aaatggacgc tagcgccagc ttttcgctgt gcttccatca
     2701 gatgaacgag ataagccgat gttcgtccac ctgaaaagct gacgacttga tgcatactca
     2761 cggtttattc ctgaacgcgc ttaaactcga ttacccatac ccacgggtta gatagccagt
     2821 tatcctgccc gtaaatactg cgccagatat caccaaaacc aacgcgataa acagcttccg
     2881 gcggcgtaat tttgtacatc gccggagatg cgccttctgc tttagcatct gcctcgctca
     2941 tatcacgcag ccgttccaca cgcacgtcgg taatttccag caaaatgcgc gatgcccagc
     3001 gcggcatgtg aattgatgga caccacccgc catcaaattt ttcattcaca gtgtgaggtt
     3061 tccagtcggc atcatcgggt atcgaccaca agccgtaatc acccggcttt tgctcacaac
     3121 tggcccgata aatccttgct gcgttcttct catcgccacg acaaaggttg tcgttccagt
     3181 ccacactgca accatcctca ttgcctaata tcgcccacgc ctcacggacc catatgcgat
     3241 caccaacgtt accgaacggg cataaaccat gttcaggtgc atccataacg tgggtataga
     3301 caccattgcg tttctttgtc ggtttacgga taaccaggca gttatcggcc ctgacatttt
     3361 ttacaggtcg gcgagtctgc gtcttccttc cttcgaggat ggccctgacc atctcatcgt
     3421 taaaaatcat cccgcgttct ttcatttttt gatccacctc atcagttatt gaaagtgatc
     3481 actccgcgct cgatggcgaa gtcaaaaagc tggttagcgg ctacgtaaag gcgtatgcca
     3541 tgtgcttttt cccatgcccg gacatcgttt tctgcgcttc tggcgcattc atcgcaaaga
     3601 ggaacagccc agcggtcgtg ttcgtttaac gagcgagcgc ggtacatgaa tgggtggtta
     3661 actttgccac cgcatccgat gcacggacga gaaactacaa accggagata agccgggctt
     3721 ttaccgagga ccgcttttgg tcggcgcata taaagcaggc cggaatcctc atctacagac
     3781 aggttgacga tctgctctgc ggttatgtcc accagctcgc gggtactgtg ctcccaggtg
     3841 atatctgatt ctttcagcgt accggtaggg atttcagttt ttggctgaca aaatgcaatg
     3901 cgaccggctt catctggcag ctcatctttc agattccggc gaatggccca aaaagtgagt
     3961 tcaaccatgc tcagatcgcg ttctggcggt a
//
LOCUS       DBBGLQ010000228         3982 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.228, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000228 DBBGLQ010000000
VERSION     DBBGLQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.228"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            76..642
                     /gene="ybgD"
                     /locus_tag="ACIYSB_005318"
     CDS             76..642
                     /gene="ybgD"
                     /locus_tag="ACIYSB_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGC5432129.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HPEDIDKNIDLGQVTTTHINREHHSNKVAVDIRLINCDLPASDNGSGMPVSKVGVTFD
                     STAKTTGATPLLSNTSAGEATGVGVRLMDKNDGNIVLGSAAPDLDLDASSSEQTLNFF
                     AWMEQIDNAVDVTAGEVTANATYVLDYK"
     gene            693..3149
                     /gene="ybgQ"
                     /locus_tag="ACIYSB_005319"
     CDS             693..3149
                     /gene="ybgQ"
                     /locus_tag="ACIYSB_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGC5432130.1"
                     /translation="MDTVNIYRLSFVSCLVMAMPCAMAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNKISNGQKINWQKKGDKTIPCINDSLVDKFGLKPDIRQ
                     SLPQIDRCIDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWAPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINAGAWRLRSDYQLNKTDSEDNHDQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISDDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTIHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAASDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQLFTVVWGEQSC
                     IIHLPERLEDTTKRLILPCH"
     gene            3164..3892
                     /gene="ybgP"
                     /locus_tag="ACIYSB_005320"
     CDS             3164..3892
                     /gene="ybgP"
                     /locus_tag="ACIYSB_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGC5432131.1"
                     /translation="MTFIKGLPLMLLTISLGCNAAVQPDRTRIVFNANDKATSLRIEN
                     QSDKLPYLAYSWIENEKGEKSDALLVALPPIQRLEPKATSQVRVVKQASTTQLPGDRE
                     TLFFYNMREIPPAPDKSSDHAILQVAIQSRIKLFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAK"
     gene            3889..>3982
                     /locus_tag="ACIYSB_005321"
     CDS             3889..>3982
                     /locus_tag="ACIYSB_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432132.1"
                     /translation="MSAGKGLLLVICLLFLPLKSAMALNCYFGTS"
BASE COUNT         1078 a          981 c          952 g          971 t
ORIGIN      
        1 taaaaactag caataatcaa atacgatatt ctggcgtagc tataccccta ttctatatcc
       61 ttaaaggact ctgttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
      121 ctgttcactg cacctgttta tgctgctgat gaaggttctg gcgaaattca ctttaagggg
      181 gaggttattg aagcaccttg tgaaattcat ccagaagata ttgataaaaa catagatctt
      241 ggacaagtca cgacaaccca tataaaccgg gagcatcata gcaataaagt ggccgtcgac
      301 attcgcttga tcaactgtga tctgcctgct tctgacaacg gtagcggaat gccggtatcc
      361 aaagttggcg taaccttcga tagcacggct aagacaactg gtgctacgcc tttgttgagc
      421 aacaccagtg caggcgaagc aactggggtc ggtgtacgac tgatggacaa aaatgacggt
      481 aacatcgtat taggttcagc cgcgccagat cttgacctgg atgcaagctc atcagaacag
      541 acgctgaact ttttcgcctg gatggaacaa attgataatg cagtcgatgt cacggcaggt
      601 gaagtaaccg ctaacgcaac ctacgtgctg gattataaat aagtattatt ttggcggcat
      661 catcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
      721 cttttgtatc ctgcctggtc atggcgatgc cttgcgcaat ggcggtcgaa ttcaatctga
      781 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
      841 tcattgctcc cggtgaatat tttgttagcg ttgcggtgaa taacaacaaa atcagtaatg
      901 ggcaaaaaat taactggcaa aaaaagggtg acaaaaccat tccatgcatc aatgattcac
      961 tggtcgataa atttggttta aaaccagata tccgtcagtc cttgccacag atagatcggt
     1021 gtattgattt cagttcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
     1081 tgaatattag tattccgcaa gcctggctgg cgtggcactc agaaaactgg gctcccccct
     1141 ctacatggaa agaaggtgtt gccggtgtcc tgatggatta caacttgttt gccagcagct
     1201 accgcccaca ggacggcagc agcagcacta acctgaatgc ctacggtacc gccggaatta
     1261 acgccggggc atggcgctta cgcagtgatt accagcttaa taagaccgat agcgaagata
     1321 accatgacca gtcaggcgga atatcgcgca cctatctttt tcgtccatta ccgcaattag
     1381 gctctaagtt aaccctcggc gaaaccgatt tcagttccaa tattttcgat ggtttttctt
     1441 ataccggcgc ggcactggcg agtgacgatc gaatgttacc gtgggagctg cgtggctacg
     1501 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
     1561 gcgtcattta ccagaaaaaa gtcccgccag gcccgtttat tattgatgac ctcaatcagt
     1621 ctgttcaggg cacgctggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
     1681 aggtttcggc agcatcgacg cccttcctga ctcgccaggg acaggttcgc tataaattgg
     1741 ccgcgggtca gccacggcct tccatgtcac atcaaactga aaatgaaacc ttttttagca
     1801 atgaagtttc ctgggggatg ctctcaaaca cctcgctgta cggcggcctg ctgatttctg
     1861 atgatgacta ccattctgcc gcaatgggta tcgggcaaaa tatgctgtgg cttggcgcac
     1921 tgtcctttga tgtcacctgg gccagtagcc attttgatac tcagcaggac gagcggggct
     1981 taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg atttcgctcg
     2041 ccgcttatcg cttctcagat cgtcattttc acagctacgc caactatctg gatcacaaat
     2101 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatccgtg ggccaaccga
     2161 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
     2221 acgcctccac gaccgccaac atcacagcag gttttaatgt tgatattggt gactggagag
     2281 atatctcgat ttcgacgtca ttcaatacga cccactacga agataaagat cgcgacaacc
     2341 agatttatct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
     2401 aaaacagtag ccacagcacc atacaccgca tgtcgtggaa cgatacgctg gatgaacgta
     2461 atagctgggg catgtctgcc ggactgcaat ccgatcgtcc ggacaatgga gcccaggtga
     2521 gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt acctatgccg
     2581 ccagtgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
     2641 gtgcagcatt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
     2701 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
     2761 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
     2821 ccgacggcgt aacagttgca gaaaacgtca tcaaggaaac atggattgaa ggcgcgatag
     2881 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcata cgcaacgcca
     2941 gcggtcagtt ccctcccctc ggtgcggata tccgccagga tgacagcggc attagcgtgg
     3001 gtatggttgg cgaggaagga catgcctggt taagcggtgt cgctgaaaat caactgttta
     3061 ccgtggtctg gggtgagcaa agctgcatta ttcatctgcc agaacgtctg gaagacacga
     3121 ccaaacgcct gattttacct tgtcattaat taaggaaaag agaatgacat ttattaaagg
     3181 attaccctta atgttgttaa ccatcagctt gggttgcaat gctgcggtgc aacccgatcg
     3241 cacacgcatt gtgtttaacg ccaatgacaa agccaccagc ctgcgtatcg aaaaccaaag
     3301 tgacaaactg ccttatcttg cttattcctg gatagaaaat gaaaaagggg agaaaagcga
     3361 tgctcttctg gtcgctttgc cccctattca acgtctggaa ccgaaagcga catcgcaggt
     3421 gcgggttgtg aaacaagcct caaccacaca attacctggc gatcgcgaaa cgctattttt
     3481 ctacaatatg cgcgaaattc ctcccgctcc ggataaaagc agcgaccatg cgatacttca
     3541 ggtcgctatt caaagtcgta ttaaattgtt ctggcgaccg gctgcattac gcaagaaagc
     3601 aggagaaaag gtcgaactgc agttacaggt cagccagcag ggcaaccaac tgacactgaa
     3661 aaatcctacc gcgtattatc tgaccattgc ctatctgggg agaaatgaaa aaggcgttct
     3721 cccaggcttc aaaaccgtga tggttgcacc ttttagtacg gtcaacacaa acaccggaag
     3781 ttatagcggc agccagtttt atctcggtta catggatgat tacggcgcat tacgcatgac
     3841 gacgctcaac tgcagcggac aatgccgttt acaagcagtg gaggcgaaat gagtgctggc
     3901 aagggattgt tgctcgtcat ctgcctgtta tttctgccgt tgaagtctgc aatggcgctg
     3961 aactgctatt ttggtacatc tg
//
LOCUS       DBBGLQ010000229         3883 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.229, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000229 DBBGLQ010000000
VERSION     DBBGLQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.229"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            245..814
                     /locus_tag="ACIYSB_005322"
     CDS             245..814
                     /locus_tag="ACIYSB_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432133.1"
                     /translation="MIQDLLIEAISHDRMHKKLNELNCYFYNRKHETQIRDELVVILN
                     QISTLTALSEHPKLGIGAVDISLYNQSILTSEHNGNVATIEIKHHYPKDLLYRQVQED
                     IISDISRVIVSPTTHFIHIIQQRTRINTPSFGQVKFLERDASDISTYVQCLEELSSFP
                     SIFHKKSICIEVLGEIMSTYTFNVYSFDN"
     gene            complement(836..1093)
                     /locus_tag="ACIYSB_005323"
     CDS             complement(836..1093)
                     /locus_tag="ACIYSB_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017898846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HGC5432134.1"
                     /translation="MMVVLHALCLLPLLSGCGSTRTVYVSTPATTLPTSLTSETPVPF
                     IPNPLTYGASLELNVSLLSALANCNRDKADIRKIDAERTNH"
     gene            complement(990..1370)
                     /locus_tag="ACIYSB_005324"
     CDS             complement(990..1370)
                     /locus_tag="ACIYSB_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HGC5432135.1"
                     /translation="MKLSYKLVIAAFFFTVIGSFIWSANHYYSKYQHEKKRADEAVQN
                     AESATAITRNVLQSLQIVNTVIEVNQHAKQQIALESQRTQKDIKVAVADDGCAARLVP
                     AAAAERLRKYADSLREHSGYNITD"
     gene            complement(1354..1830)
                     /locus_tag="ACIYSB_005325"
     CDS             complement(1354..1830)
                     /locus_tag="ACIYSB_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGC5432136.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGKLFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEVEL"
     gene            complement(1834..2160)
                     /locus_tag="ACIYSB_005326"
     CDS             complement(1834..2160)
                     /locus_tag="ACIYSB_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HGC5432137.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            2452..3627
                     /locus_tag="ACIYSB_005327"
     CDS             2452..3627
                     /locus_tag="ACIYSB_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGC5432138.1"
                     /translation="MDITCLECGNVLDDPTVACDKCGATPHVVVLGKQSYFPIGAVTA
                     NLEKNDSRAFDYRLGEVWDLKNEVTSEFITRIEKKFSRKNKFHNFLDSDHNPSSIPTI
                     LKKYINKNNEFIDLSRAIIEKLKHNANNESRVAQLQGGSVVFIHYKSTEPEDLGKLLI
                     VMVDKQSAYDFDSDKLTPTRLNPINTDALRQAAMFDLTLFEASYPENKGDSYVHFLQG
                     KSKSDFFKDSLGCRHDSDNKRSIQQLFSAIDIFASKNSLGRVLRDTIDNEVKSLLEKK
                     SKDKYGNKSVKIEDISKIIDSCLTDSHKCKGTFVDFVNLNGFQIDPQFEPTPKAAESA
                     LTIEVADNDNNFKLKIMRGAIGDEKSNKPVILTDNKCEIVIKLSQANYDELKRYRDS"
     gene            3630..>3883
                     /locus_tag="ACIYSB_005328"
     CDS             3630..>3883
                     /locus_tag="ACIYSB_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432139.1"
                     /translation="MTIADDLSRLAQIINGASSRVEGYYTVISLEESIVIVNSSEIIR
                     LLQSIGYKKATTCIENNEIWLDRQASSWDDAIIYENVESF"
BASE COUNT         1228 a          812 c          779 g         1064 t
ORIGIN      
        1 cgccctgcaa taatcgaaat attagaaaca accactacaa ctgtggaggc gatgatcgca
       61 catctgaata gtatcgatag tatttaccat gaaccgattt acaaagcggt ttacgagtgg
      121 gccgtaaaga ttttctatcg atgatacttc gttatcttgc tggcattcac aataccttta
      181 catactaatg acatgccagc acaatactgt cacttacaac cgttcggatt acctagagaa
      241 aagtatgatt caggatttac tgatcgaagc aattagtcac gataggatgc ataaaaagtt
      301 aaatgaactg aattgctact tctacaatcg caaacatgaa actcaaatac gtgatgagtt
      361 agttgttatt ctcaatcaaa tcagcacact aactgcttta agtgagcatc caaaactcgg
      421 tatcggcgct gttgacatat cactttataa ccagtcgata ttaacgtctg aacataatgg
      481 caatgttgca accatcgaga tcaaacacca ttacccaaag gatttacttt atcggcaagt
      541 tcaagaagac atcatttccg atatttcaag agtaatagtt tcaccaacta cacattttat
      601 ccatataatc cagcaaagaa ctaggattaa tactccttct tttggccaag tgaaattcct
      661 tgaacgtgat gcaagtgata tcagcactta tgtgcaatgc cttgaagagc taagttcctt
      721 ccccagtatc tttcataaaa aaagtatatg tattgaggta cttggtgaaa ttatgtcgac
      781 atataccttt aacgtatatt catttgacaa ctgattaatt tgacaatttt attgcttaat
      841 ggttagttct ctctgcatct attttacgaa tatcagcttt atcccgattg cagttagcca
      901 gtgcagacaa cagactcaca ttcagctcca ggctggcacc ataagtcaga ggatttggta
      961 tgaacggtac aggtgtttca gaagtcagac tagtcggtaa tgttgtagcc ggagtgctca
     1021 cgtaaactgt ccgcgtactt ccgcaaccgc tcagcagcgg cagcaggcac aaggcgtgca
     1081 gcacaaccat catccgcaac agccactttg atatctttct gggttctctg tgactccagt
     1141 gcgatctgct gttttgcatg ctggttaacc tctataactg tattgacgat ttgcagtgat
     1201 tgcaggacgt tacgggtaat ggctgttgca gattcagcat tttgtacagc ctcatcagca
     1261 cgtttctttt cgtgctgata tttgctgtag tagtggttgg cagaccagat gaaagaaccg
     1321 atgacagtaa agaagaatgc agcgataacc agcttatagc tcaacttcat ttaccacccc
     1381 accagcctct ttaaatcggg caatcaggtc accgatttta tgttcatact gaccgtaacc
     1441 tgcaccaggt aacgacgccc agatattgct gcaacggtcg attgcctgac gaatatcacc
     1501 gcgatcaatc atcggtaaag cgccacgctc tttaatctgc tgcaatgcca cagcatcctg
     1561 gcttttggga gagaagtctt tcagagcaag ctgcttacgg taggcatccc accagcgtga
     1621 aagaagctgg taacgtccgg cggctgttga tttgagtttg gggtttagcg tgacaagttt
     1681 gcgagggtga tcggagtaat cagtgaacag tttgccacca acaataacat cataaccgtg
     1741 gttacgtgtc ggttgtcgcc cgttatccgt tccttctgac caagccacca tatcaaggaa
     1801 agctttacgc tgggaattta gtacctgcat aaattactcc ttagagccac caaatttgtt
     1861 accgattact cgcattgcag ccccacgaat agcatcgaca ccgatcagcc ccactccacc
     1921 accaatggca acagatagtg atttaggcca tccgacatac tcaagagcgg atgcaaaagt
     1981 cagcgtcaga gcgccacaga gtagaatttc gagtgttttt cgcttccagc caccaccacc
     2041 gccaaaatag gcaatacgta aaccagccat aacaatcgac ataatcactg cgcccagcgg
     2101 tgtgtctcca cgccaccagc tctggaccaa ctccaaccag gtatttgggt tatgaggcat
     2161 ttgtagttat ctctcacctc gctgatacag caggtgcaaa ttgaggaaac atcatgtacc
     2221 gcaaatcaga agcggaaacg tcaaagaagc cgagtcaatg gataactgcg ggataggcca
     2281 ggaccaagga atccccagac ccagaaacga caaaacccgc tcgatggcgg gtttaagctg
     2341 tgtggcgaag tgagcacact taacagatta cgatattttt tgcgtacgcg ttagtgtttt
     2401 gatagaattt tcagccgcat aaaaattcat tcttataatt cgggatatat aatggatata
     2461 acttgtttag aatgtggcaa tgttcttgac gacccaactg tagcttgcga taaatgcggt
     2521 gccacgcctc atgttgtagt gctgggcaaa caatcgtact ttcctattgg tgctgtaaca
     2581 gcaaatcttg aaaaaaatga ttcaagagca tttgattatc gattaggtga agtttgggat
     2641 ctaaaaaatg aagtcacatc tgaattcata accagaattg agaaaaaatt tagccgaaaa
     2701 aacaaatttc ataacttcct agactcagat cacaaccctt catccattcc tacgatccta
     2761 aaaaaataca ttaataaaaa taatgaattc attgatttat ctagagctat aatagagaag
     2821 cttaaacata atgctaataa cgaatcgaga gttgcccaac ttcagggggg gagcgttgtt
     2881 ttcatccact ataagtctac cgaaccagag gatttgggga aacttctaat cgtaatggtt
     2941 gataaacaaa gcgcctatga ctttgattcg gataagttga ccccaacaag attaaatcca
     3001 attaataccg atgccttacg gcaggctgcg atgttcgatt taactttatt cgaagccagt
     3061 tatccagaaa acaagggtga ctcatatgta catttcttgc aaggtaaatc taaaagcgat
     3121 tttttcaaag actcattagg ttgccgacat gattcggaca ataaaagaag cattcagcaa
     3181 ttattcagtg ctatagatat ttttgctagt aaaaactcac tcggtcgtgt actgcgtgat
     3241 actattgaca atgaagttaa atccttactg gaaaaaaaat caaaagataa atacggaaat
     3301 aagtccgtta agatagagga tatttcaaaa ataattgact catgcctgac tgattcacac
     3361 aaatgtaagg gaacgtttgt cgattttgtt aatcttaatg gtttccagat tgaccctcaa
     3421 tttgaaccaa ctcctaaagc agctgaaagc gcgcttacaa ttgaagtggc agataacgac
     3481 aataatttta agttgaaaat aatgcgtgga gctattggag acgaaaagtc aaataagcct
     3541 gtaattctca ctgacaacaa atgcgaaatt gtgataaaat tgagtcaggc aaattatgat
     3601 gaactcaaaa gatatagaga cagctaataa tgacaattgc tgatgactta tcaagattag
     3661 cgcagattat taacggagcc tcaagcagag ttgagggcta ctacactgtc ataagtcttg
     3721 aagaaagcat tgttattgta aatagttctg aaataattag actgttacaa tctataggtt
     3781 ataaaaaggc aacaacctgc atcgaaaata atgaaatttg gctagatcgc caagcttcat
     3841 cttgggatga cgctataatt tatgagaatg ttgagtcttt ttg
//
LOCUS       DBBGLQ010000230         3846 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.230, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000230 DBBGLQ010000000
VERSION     DBBGLQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.230"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            74..442
                     /locus_tag="ACIYSB_005329"
     CDS             74..442
                     /locus_tag="ACIYSB_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGC5432140.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(545..946)
                     /gene="pliG"
                     /locus_tag="ACIYSB_005330"
     CDS             complement(545..946)
                     /gene="pliG"
                     /locus_tag="ACIYSB_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGC5432141.1"
                     /translation="MKIKSIRKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            1188..1481
                     /locus_tag="ACIYSB_005331"
     CDS             1188..1481
                     /locus_tag="ACIYSB_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGC5432142.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1553..2212
                     /locus_tag="ACIYSB_005332"
     CDS             1553..2212
                     /locus_tag="ACIYSB_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGC5432143.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLSLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            2304..2750
                     /locus_tag="ACIYSB_005333"
     CDS             2304..2750
                     /locus_tag="ACIYSB_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGC5432144.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2957..>3846)
                     /gene="hlyE"
                     /locus_tag="ACIYSB_005334"
     CDS             complement(2957..>3846)
                     /gene="hlyE"
                     /locus_tag="ACIYSB_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HGC5432145.1"
                     /translation="VADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELS
                     RFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNE
                     KKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSS
                     YFQSQVDKIRKEAYAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLS
                     NTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINT
                     CNEYQKRHGKKTLFEVPEV"
BASE COUNT          998 a          794 c          924 g         1130 t
ORIGIN      
        1 gtcagcggtt ttgattacgg attgatgatg aaatgagcat aagtgttgta cggttggatt
       61 atcaggatgt gctatgaata tgatgcgtat tttttatatc ggattgtcag gtgtggggat
      121 gatgttctca tcaatggcgt ctggaaatga cgctggcgga cttcaatctc cggcgtgcgg
      181 cgttgtttgc gacccgtata tttgtgtgaa ctcagatggc atttccccag agttaacaag
      241 gaaatatctc ggcgaaaaag ccgctgaaaa cttacaatca ttacaaggct acgatcccag
      301 cgaatttaca ttcgctaacg gtgtattttg cgatgttaaa gaaaaattat gtcgtgatga
      361 tcgttatttt ggtgtggatg gtaagcggag tggaaaaatc aatcaaacca ccacaaagat
      421 gttatttatg tgtcgtgagt gatgtcttgt ttacattatg ttctattcgt cattaacctc
      481 ttgagcgaca gagaggagtg gggcattaac gtttatgacg gggagagtcc ccgtcggttg
      541 acatttactt aatttgaata tcgacgttat attttttagt tttgttttta cgggcgtcgt
      601 ttcgggtttg caacactcgc agctcatatt tgccagaagc agggagtgag tattgtccgt
      661 gactatcgag ttctggggaa tatctggata aatcaactga atcatcgatt cctggcccga
      721 agaggtaagt atcagcgcct tcattagaga tactcacatg cactttctga ccttttttgg
      781 catagaaggt atatgtatcg taatcgtatc cctttatttc gccggagtac tgagcgctgc
      841 tatggccttt tctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
      901 gcagtgcaag cagcaataca gccttcctga tgctcttgat tttcattggt agtctcctgt
      961 tcagaatgag tacgatgaac tgttataata taacaatccc taacgggaga attccagtgg
     1021 ctttcctgcg ttcggtaaaa tcacgttgcg gcatttttgc cggggggatt acttataaag
     1081 ggggcatgga ctcaacaaac cgtagaaatc tacagctggc tggcttgtgg cgcgggtttc
     1141 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
     1201 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
     1261 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
     1321 cgctggatgg gcggaagaaa ctggtcaatg ccgatattga aaaggttaaa caggcattaa
     1381 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
     1441 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
     1501 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
     1561 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
     1621 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
     1681 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
     1741 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cactgcgtca
     1801 ggctacggaa gagcatgtcc gcaaagccat tgccggttat ggcgtggcgc tcgatctgac
     1861 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1921 gtttgataac tcttgtccgc tttccgggtt tattcccgcg gcggaattta ccggcgaccc
     1981 gcaaaataca acgctgagcc tgagcgtaaa cggcgaacaa cgccagcaag gtacgactgc
     2041 ggacatgatc cataaaatcg ttccgctgat cgcttatatg agcaagtttt ttaccctcaa
     2101 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     2161 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     2221 gccgcctgaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     2281 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     2341 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcctgc
     2401 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     2461 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     2521 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     2581 atcgtttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     2641 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     2701 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     2761 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     2821 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2881 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2941 agagaataat cgcttatcag acttcaggta cctcaaagag tgtcttttta ccgtgtcttt
     3001 tctgatactc attacaggtg ttaatcattt ttttggccgc ttcttttagc aaagaaagca
     3061 ttaaatcatc ataatcaacg tagaatctgg ttgtttcagt ttccgttttt atctcaccga
     3121 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     3181 taaccgtgtt agacagggtg gtaaagaaat tctgcacaga ttttaacttg ttcttcaatt
     3241 ctggaatcag ttttccttca actacgcccg cagcaataga ataggaaatg attaatccaa
     3301 atggaccggc gacgacaccg gcatatgctt ccttcctgat tttatctacc tgtgactgga
     3361 aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc agcagtttcc
     3421 cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct tcattcagct
     3481 tcgtgatgcc gtcatccagt accttaatga gaatgtcttt ctgggcggat gctttcttct
     3541 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     3601 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     3661 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     3721 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     3781 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3841 caacga
//
LOCUS       DBBGLQ010000231         3822 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.231, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000231 DBBGLQ010000000
VERSION     DBBGLQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.231"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..257
                     /locus_tag="ACIYSB_005335"
     CDS             <1..257
                     /locus_tag="ACIYSB_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432146.1"
                     /translation="NIFRMAVRTWVKSSDSSYPVSVVIALELYSVDAERVSLRVRVED
                     DISVRVFINKPSEEAWGDVVMHMKKHIANHLKKRAPSALR"
     gene            complement(324..1379)
                     /locus_tag="ACIYSB_005336"
     CDS             complement(324..1379)
                     /locus_tag="ACIYSB_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGC5432147.1"
                     /translation="MSRKNKQRRTNARAAQGSLADSLRSDPTLSAFTFEGPYRLSGAD
                     LLDSMYCADNGRWFETPVDWYGLARAARQTSWHQSALSFKRNVLLGCYIPHPLLSRQE
                     FAGVAMDWFVFGNACLELRRNMLGEPLALRRALAKYMRRGSDMKSWWYIQEGQEEYRF
                     REGTICHLMNPDINQEIYGMPDYLGGLLSASLSHSADIFRKLYYDNGSHAGCIVYIGA
                     AQVDRESMEKLRETLQSARGGGAFKNLLIHAAGGGKDGVQILPFQQITAKDEFMNVKA
                     ASRDDVLSVHRVPPQLMGAMPGEKGSFGDVEKAARVFAINELMPVMEAMKHVNDWLGE
                     EVIRFNPYALLDTQPIS"
     gene            complement(1379..3130)
                     /locus_tag="ACIYSB_005337"
     CDS             complement(1379..3130)
                     /locus_tag="ACIYSB_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGC5432148.1"
                     /translation="MIQDTFVRQRARQLYWQGYPVAEICRLMGINQNTIHSWKKRDQW
                     DETPPVQRVTQSMDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDDLPDETATGKKG
                     RAKKRKNHFTPEQIAALREKITDRLAGHQRKWLDAIGACQRAGITDRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQALQFKSIIQKAASEVDVELKGGDKV
                     ILSNGAELHFLGTSAATAQAYTGNLYFDEFFWTGRFAELRKVAAAMATLDGLRCTYFS
                     TPSSETHEAYAYWNGDHWNEGLRTHKRQHFDVSWNALHNGLICPDYIWRQIVTLEDVV
                     SQGWTLTNIDKIRRNNTEDEFRNLYMCEFAREGESAFNLNILIGCGVDGYDEWPDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVVVVVPPAVPGGRFRTVETRRVQGLEFEAQ
                     AKVIEEFTFRYNVEHIGIDVTGGHGDAVYQIVKRFFPAAIPYTFTLSSKRTLVLKMLQ
                     LMRAGRWEYDRSERELVSAFNAVRKTKTQAGFITYETDRARGVSHGDLAWATMLAVIN
                     EPVGGEHDAAPFSVTEF"
     gene            3286..>3822
                     /locus_tag="ACIYSB_005338"
     CDS             3286..>3822
                     /locus_tag="ACIYSB_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGC5432149.1"
                     /translation="MPEQKKTSRKTFRVAVSGPTADGREISAADLRAAASNFNPEIYG
                     ARVNVEHWLSPVPSSEFSAMGDVTALSTEEITEGPLAGRTALYAEIEPTDRMKNLVSR
                     GKKIYSSIELARNFTPTGTPYLIGLAMTDTPASIGTERLQFALQQRRSVMTFNNQQTE
                     APMFSDAIEAEVIELTERR"
BASE COUNT          847 a         1140 c          968 g          867 t
ORIGIN      
        1 acaatatttt ccgcatggcc gtgcgaacct gggttaagtc atctgacagc agctacccgg
       61 taagcgtggt tattgcactt gaattatatt cagtagatgc ggaacgggtg tctctgagag
      121 tccgggtgga agacgatatt tctgttcgcg tatttatcaa taaacccagc gaagaagcct
      181 ggggcgatgt tgttatgcac atgaaaaagc acatcgctaa tcacctgaaa aagcgagcgc
      241 catcagcact tcgttaagca gagctggaga gtcggggcat aaatttttta tgccccggcg
      301 aagcagcaga caagcgaagc gcgtcaggat atgggctggg tgtctaacag tgcgtaaggg
      361 ttaaaacgga tcacctcttc gcccagccag tcgttgacgt gtttcattgc ttccatgacg
      421 ggcatcagtt cgttaatggc aaacacacgg gctgccttct ccacatcccc gaatgacccc
      481 ttctcacccg gcatggcccc catcagctgc ggcggtacac ggtgcacgga cagcacatca
      541 tcacgcgatg cagccttgac gttcatgaac tcatcctttg cggtgatttg ctgaaacggc
      601 aaaatctgta cgccatcctt accacctccc gccgcgtgga tgagcagatt tttaaacgcc
      661 ccgccaccac gcgcgctttg cagtgtttcc cgcagttttt ccatgctttc acggtcaacc
      721 tgcgccgcac cgatgtagac aatacagccc gcgtgtgagc cgttatcgta gtagagcttg
      781 cggaagatat cggcggaatg tgacaggctg gcagacagca ggccgcccag gtaatccggc
      841 atcccgtaaa tctcctggtt gatatcagga ttcatcaggt ggcatatcgt cccctcacga
      901 aagcggtact cttcctgtcc ttcctgtata taccaccatg atttcatatc gctgccacgt
      961 cgcatgtatt tcgccagcgc ccggcgcagg gccagcggtt cgccaagcat gttgcgccgc
     1021 agctcaagac aggcattacc gaacacaaac cagtccatcg ccaccccggc gaactcctgg
     1081 cgggacagca gaggatgtgg aatatagcag cccagcagca cattacgttt gaagctgagc
     1141 gccgactggt gccaggacgt ctggcgggca gcccgtgcca gtccgtacca gtcaacgggc
     1201 gtttcaaacc accgcccgtt gtcggcgcag tacatgctgt ccagcaggtc ggcccctgac
     1261 agccggtaag gtccttcaaa cgtgaaggca ctcagtgtcg ggtcgcttcg cagggaatcc
     1321 gcaaggctgc cctgcgctgc gcgggcgttc gtacgtcgtt gtttattttt tctgctcatc
     1381 agaactccgt aacactgaag ggggcggcat catgttcacc gccgacaggt tcgttgataa
     1441 cggccagcat ggtggcccat gcaagatcgc catggcttac gcctcttgcg cgatctgttt
     1501 cgtaggtgat aaatccggcc tgcgtttttg ttttgcgtac cgcgttaaag gcgctcacca
     1561 gttcgcgctc gctgcggtcg tactcccacc gcccggcgcg catgagctgc agcatcttca
     1621 gaaccagcgt gcgctttgat gacagggtga aggtgtaggg aatggctgcc ggaaaaaagc
     1681 gcttcacaat ctgatacacc gcatccccgt gaccgccggt tacgtcaata ccgatatgct
     1741 caacgttata gcgaaaggtg aactcttcaa tcaccttcgc ctgcgcctca aactccagcc
     1801 cctgcacccg ccgcgtctcc acggtgcgaa aacgcccgcc cggtacggct ggcggcacca
     1861 ccacgacaac cgccccgctg tcaccgttcc cgctactgcc gttggcgtcg tacccaatcc
     1921 acacagggcg gttccccatc ggacgcggtg caaacggctt ccagtccggc cactcgtcgt
     1981 atccgtcaac accgcagcca atcaggatat tcaggttaaa tgccgattcc ccttcgcgag
     2041 caaattcaca catgtagaga ttgcggaact cgtcttcagt gttattgcgc cggattttgt
     2101 caatgttggt taacgtccat ccctgactga ccacatcttc cagcgtcaca atttgccgcc
     2161 agatatagtc aggacagata aggccgttat gaagcgcatt ccagctcaca tcaaaatgct
     2221 gacgtttatg agtacgaagc ccttcattcc agtgatcgcc gttccagtat gcatacgcct
     2281 catgtgtttc acttgatggc gtggaaaaat aggtacaccg caaaccatcc agcgttgcca
     2341 tagcagcagc caccttgcgc agctctgcga aacgccccgt ccagaaaaac tcatcaaaat
     2401 acagattccc tgtgtacgct tgcgcggtgg ccgcagaagt gccgagaaaa tgcagctctg
     2461 caccgttgga gaggatgact ttatcgcccc ctttcagttc cacatcaact tcagatgcag
     2521 ccttctgaat aatgctttta aactggagcg cctgacgacg agacgcagac aaaaaaatct
     2581 ggttacgctg gtaaggttgt gccacatcgt cacgcagcgc catcagcagc gcttcctgcg
     2641 caaaatacca ggtcgcccca atctggcggg atttcaggat catcctgtca gtaattccgg
     2701 cacgctgaca ggcaccgata gcatcaagcc atttgcgctg gtgtccggcc agcctgtcgg
     2761 taattttttc ccgcagcgcc gcgatctgtt ccggcgtgaa atgatttttg cgttttttcg
     2821 cccggccttt ctttcctgtt gccgtctcat ccggcagatc gtcatgcagc tttttaagct
     2881 gacgggtcag caaatcaatt tccttaaagt ccccgcctgt tttattctgt ttttccgtga
     2941 gctgaatgag tcgcgcatcc atggactgcg tgacacgctg aaccgggggc gtctcatccc
     3001 actggtcgcg ttttttccac gaatggatcg tgttctggtt gatacccata aggcggcaaa
     3061 tttccgcaac agggtaaccc tgccagtaca gctggcgtgc ccgctggcga acaaatgtat
     3121 cctgaatcat ttttcccccc tcatgtgatt caggcagttt actcacccct tacaggcctc
     3181 ctgccatgcc cggtgttgtc ggcattcgct tacaacaaac gcactttgtc tgtccctgac
     3241 ccgttttgcc atcatgagac aaacgatcat cacgagggcc cgacaatgcc tgaacagaaa
     3301 aaaacatccc gtaaaacatt ccgcgtggcc gtttccggtc ccaccgcaga cggtcgcgaa
     3361 atcagcgccg cagatttgcg cgcggcggcc agtaacttca accctgagat ttacggcgca
     3421 cgcgtgaacg tggagcactg gctttcaccg gttccgtcca gtgaattttc cgccatgggt
     3481 gatgtgacgg cactgagcac agaagaaatc accgaaggtc cgctggccgg tcgtactgcg
     3541 ctgtatgcgg aaatcgaacc caccgaccgc atgaaaaatc tggtctcccg gggtaagaaa
     3601 atttactcca gtattgagct ggcacgaaat ttcaccccta cgggcacccc gtatcttatt
     3661 ggccttgcca tgacagacac cccggcaagc atcggcacgg agcgtcttca gttcgccctg
     3721 cagcagcgcc gttcggtcat gaccttcaat aatcagcaga cggaagcacc gatgttctct
     3781 gacgccattg aggctgaagt gatcgaactg acggagcgcc gc
//
LOCUS       DBBGLQ010000232         3785 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.232, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000232 DBBGLQ010000000
VERSION     DBBGLQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.232"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..2155
                     /locus_tag="ACIYSB_005339"
     CDS             <1..2155
                     /locus_tag="ACIYSB_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGC5432150.1"
                     /translation="RELIANDIEQYLNNLNKLLSGQEQKITLLKSAKTEWKKYRAGES
                     LIYSLFSWLPAVRSKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQ
                     TTYRQQIDSAHEIILKEQQAVQEWQRLALDLGYEGDEELSFSQADELADTQIRFPAFL
                     LTTHYWEGRWLMDMASIDDLQEEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQ
                     ISEHKGQRKFEKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIW
                     SIAPAIDIGNMLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDP
                     ELARGMYLYEHRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELA
                     SSGSRYNLLEAETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQG
                     ISTGTNEKSLTVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDS
                     FLVFGDMDLFEVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVE
                     QHDNFLNQTFENTSKHITIVSPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNT
                     EHNDFEKRKEKQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSAT
                     REARYERYDTSMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(2264..3763)
                     /locus_tag="ACIYSB_005340"
     CDS             complement(2264..3763)
                     /locus_tag="ACIYSB_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGC5432151.1"
                     /translation="MNIRKLFCPGDTPRILLFLFFFVISVIITIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITTLLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGIVVG
                     TTDTLREMQRYKQLSQHGADNWKILPGTPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHIQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVIVFNGDRNVPADSLKPQPLILPDRR"
BASE COUNT         1079 a          804 c          956 g          946 t
ORIGIN      
        1 gcgtgagctt attgctaatg atattgagca atatctcaat aatttaaata aattactttc
       61 cggacaagaa caaaaaatca ctctactgaa gagtgctaaa acggaatgga aaaaatatcg
      121 cgccggtgaa tcactgatct attcattatt ttcctggctt ccggcggttc gcagtaagcg
      181 acagtaccaa atacagctgt ttctcgaaga taaattaggt gcgctgattg caggaaatca
      241 atggtctgat cctgaaacta tcgaacgtaa tattgatggg ctgctcaatt ccgctgagcg
      301 cgagcaaaca acataccggc agcagattga ctccgcccat gaaatcattc ttaaagaaca
      361 gcaggcggtt caggagtggc agagactggc tcttgattta gggtatgagg gcgacgagga
      421 actgagcttc tcacaggccg atgaactggc tgatacgcag attcgcttcc ctgcattttt
      481 actgacgact cactactggg aaggtcgttg gctgatggat atggccagca ttgatgatct
      541 gcaggaagag aagaagaaaa aaggcgctaa aggggtaacc gcccgttggc aacgtcgaat
      601 gaaactcact ccatgtgtgg tgatgacatg ctatatgctg cccggcaata tgcagataag
      661 tgagcacaaa ggacagcgta aattcgagaa aagttatttg tatgattttg ccgatttact
      721 cattgtcgat gaagccgggc aggtgcttcc tgaagtggct gctgcctcgt ttgcattagc
      781 taagaaggca ttagtgattg gcgatacgga gcagatcccg ccaatatgga gtattgctcc
      841 tgcgattgat atcggtaaca tgctggcgga aaaaattctg tctggcagta cgcaagaaga
      901 gattaccgag aaatatacgg caatcgcaga ccttggtaaa agtgccgcat ctggcagcgt
      961 tatgaaaata gcgcagtttg cttcgcgcta tcaatatgat cccgaactgg ctcgtggtat
     1021 gtacctatat gaacaccgcc ggtgctacga caatattatt ggatactgta atacgctctg
     1081 ctatcacggt aagttgttgc ctaaaagagg gcgtgaagag agcaatttaa tgcccgcaat
     1141 ggggtatctc catattgatg gtaaaggaga gctggcaagt agtggaagtc gatataattt
     1201 gcttgaggct gaaacgatag cggtctggtt ggcagagaac cagcaaaata ttgaagcgca
     1261 ttacggtaaa tcgcttcatg aagttgtcgg tattgtgacg ccttttagcg ctcaggtatc
     1321 cactatcaaa caggtgctgg gcaaacaagg tatcagtaca ggcacgaatg aaaagtcgct
     1381 cacagtgggc accgtgcact ctcttcaggg agcggaaaga gcgattgtga tattctcgcc
     1441 agtctattca aaacatgaag acggcgggtt tattgatagc gataacagca tgctgaatgt
     1501 tgcagtctcc cgtgcgaagg acagttttct ggtcttcggc gatatggacc tgtttgaggt
     1561 ccagccagcc tcatcgccac ggggattact ggcaaaatac ctctttgagt cagagaagaa
     1621 tgcgctctct tttgattata aagagcgtaa ggatttaaaa accgccggga ccaaaatcta
     1681 cacacttcat ggtgtggagc aacatgataa tttcctgaat cagacatttg aaaataccag
     1741 taaacacatc acgatagttt ctccatggct gacctggcaa aggctggagc aaaccggttt
     1801 tcttgattcc atgattgcgg cgtgttcacg tggaattaac gtcacgatag tcactgacag
     1861 aagctacaac actgaacata atgattttga gaagcgaaaa gagaagcagc agaactttaa
     1921 agcggcgctg gagaaactga atgcgctggg tattgctaca aagctggtaa accgtgttca
     1981 tagcaaaatt gttattggtg atgatggttt gctgtgcgtg ggatcgttca actggtttag
     2041 tgcgacacgg gaagcgcgat atgaacgata cgatacatca atggtttatt gcggtgataa
     2101 cctgaagggt gagattgagg ctatttataa tagtcttgag aggcggcagg tttagagcag
     2161 gaatggtgag tagccatctt accgatcgtt ttcgagcgta agatggctga atggaatggc
     2221 tattattgca cagtccttaa ttataacatt cataccgaca tgattatctt ctgtccggaa
     2281 gaatcagagg ctgtggtttc agactgtctg ccggtacatt cctgtctccg ttaaaaacaa
     2341 taacgggttc attatcttcg tctgtcagcg gattgaatgg cggtatattt tcagtacgaa
     2401 tgccggtcag ccactgaaaa atacctgcga aatgacgggc actgattttt ctgctgacgg
     2461 actgatgaga cgtgatgtca ctggcggtaa taatcagggg aacgctgtag cctccttgta
     2521 tatggccatc atgatgaaca ggattagcac tgtcgctgac cgacagacca tggtcagaaa
     2581 agtaaagcat ggcaaaatga cgggagtgcc ggcgaaggat accatcaagc tgaccgagaa
     2641 agttatccag tttactgatg ctggcgaggt aacaggcaat ctttcgggga tactgctcca
     2701 ggtaatgatt cggccaggag ttaagtcggt cacacggatt cggatgagag ccaatcatgt
     2761 gcaggaatat caccttcgga gaggatttat ccgccagtgc acgttctgtt tcctgtaaca
     2821 acaacatgtc atccgtttta cgggaagcaa agctgccttt cttgaggaaa acggtatgct
     2881 ccgcatcaga agcaataaca gagatgcgtg tatcatgctc ccccagcttt ccctgattgg
     2941 atatccacca tgtgctgtat cctgcttttg ctgccagcgc caccacgttg ttgccggagt
     3001 cagggttctg ctcatagtca taaatcagtg tccggctcag ggaaggtacg gtactggctg
     3061 ctgccgatgt atagccgtca ataaataaac cgggagcagt attcagccac ggtgtggttg
     3121 gcacgggata gccatatacc gacatataat ccctgcgcac actctcacca gtaacgataa
     3181 caatcgtgtc atacaacggt gttcccggca ggattttcca gttgtcagcc ccgtgctgac
     3241 tcagttgttt ataacgctgc atttcacgca atgtgtcagt tgttcccaca acaattcctt
     3301 taaccatccg caacggccag ctgtttacag agcataatac gaacagcagc agtgccagcc
     3361 agttacggtg accgcggtgg tgtgtccgcc agaaaatcac catgaatacc agaatcgcgg
     3421 cactgaccag aaaatgataa acaggaatca tcccggtaaa ctctgcagcc tcatcagccg
     3481 tggtctgcag caacgcgaca ataaaactgt tgttgatttt accgtacgtc atacccgcag
     3541 gcgcatacag tgcacaacag aacagaaata acagcgttgt aatggatgtg agggtatttc
     3601 tgtgtgcaag gagcagaagg agaaacagaa gcagcacatt tcctgtggca ttcttctcag
     3661 tgtatccgca tgcaattgtg attatcacag aaataacaaa aaagaataaa aacaataaaa
     3721 tccggggtgt gtcgcccgga caaaacagtt ttctgatatt cataggagta tatcgacaac
     3781 attat
//
LOCUS       DBBGLQ010000233         3719 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.233, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000233 DBBGLQ010000000
VERSION     DBBGLQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.233"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            240..413
                     /locus_tag="ACIYSB_005341"
     CDS             240..413
                     /locus_tag="ACIYSB_005341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432152.1"
                     /translation="MNGFKNDMDDEKSLKGKGSTEHSNKTPETQPERFLVMQINNNPK
                     TYSLITQLNHGCE"
     gene            1760..2548
                     /locus_tag="ACIYSB_005342"
     CDS             1760..2548
                     /locus_tag="ACIYSB_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432153.1"
                     /translation="MKQYKGGYNVNRYDWKNKTTEHLLNDNNLADNHIPEWIRKNEIA
                     AFWFWLIMSHTVPEKLKLNNESEFNSALKGVIVMKDNPMTIIPSSHETRLNVIGKWIN
                     ALILYGEADRIISIMHQNWLGLEKRNEAKWLKKNTEQLKWAWEYIDARISPTYLSWFN
                     PVNDNERYIAIVVLLKMLFPTDTPCLLTPKEFHLGFAAKERFFDKMHNAFRKQFIDGK
                     KDKRVQINVKISPSAKSALDRLTRERKTTQQAILEQLILYGRLD"
     gene            complement(2545..3495)
                     /locus_tag="ACIYSB_005343"
     CDS             complement(2545..3495)
                     /locus_tag="ACIYSB_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432154.1"
                     /translation="MASLSINTLRRMSICKSQAWKDTQRILKRSGLVYRGERSESFDP
                     EKHFNRYTVRYLYLLNIIALKIRSVSKFSCWIEVGQCHRMTGKHLSLNAPPFMLIPRS
                     IRRKVDGFRQSKGEATPKSAQPFTGSLYEVLRKESDSAKLDAWIAELPLNLQEEGKEI
                     SVMGFSPRAVARPVLRSASPTFELFYQEYQSLRLKSVFKFEPRFEQLLTRLSFRKQSI
                     LRKQQYRVKSQLLERLGNVLFFTSLYNQGCLEEFINALVEKEDIYLKMSPGEEKVKAH
                     QKMVNYIEAFCNKMTEKYLMPAASRHYKKKKIARSESGES"
     gene            3601..>3719
                     /locus_tag="ACIYSB_005344"
     CDS             3601..>3719
                     /locus_tag="ACIYSB_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGC5432155.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHSVIHYEPV"
BASE COUNT         1214 a          810 c          675 g         1020 t
ORIGIN      
        1 tgcgccaggg cactgttgag aaggctgtca aaacgggata accagtaagc attgtgtatt
       61 ggttgattca tgatgagaga tctccgttga tttggattga cttcacatca ttaatcactg
      121 cggtaaaact ttacggcatc ccgccattga gcaacgccaa tggctcctga ttaaaacaga
      181 atgaatcccc cacgaagaaa agataaacct acacgcagga taaaacacgc aacaacagca
      241 tgaatggatt taaaaacgac atggacgacg agaaaagcct gaagggaaaa ggatccacag
      301 aacattcgaa caaaacacca gaaacgcaac cagaacgctt cctggtaatg caaatcaaca
      361 acaaccccaa aacctacagc ttaatcacac aactcaatca cggatgtgaa tgacacgttt
      421 aaaacctgtt tacagataaa ccccgccaca agaacaaaaa tcaccaacat cagaatcgat
      481 tttctcatcc tgaaaaagtt acgtttcact acgcaccata aaaacggtta accatcgata
      541 acgtaaacct tatctgaatc aaaaacacca aaaccgttca tcacactttc gcaatgcaag
      601 aagaaacctc atgacaaaaa gaatgacctt acacggaaaa gaaaacgtca ggaatcgtta
      661 atgcctcaat ctgattgtta tcaaaacatc cagaaagtcg tttaacagaa tcgtcgtaag
      721 cacatcgaaa aaaacgtcag attacttcag gaaagttcgg gttgatttag cgtaaagata
      781 cttttctcat gctaacgtcg aaactcgaaa tctcagaaaa ggttaaacgc aacatcagta
      841 aaatcgaata gcagattgat tgcttaaaca gaaaggtttt acagcagctt taaactgatt
      901 ttagctaacg taaagattta ctaaactgga tgtgtttcag acaaagcaat gtgtacagca
      961 atgcttaatg aaaacactta tagggctgtt tatgaaaaca cgtgtatgtc tggtaaaaca
     1021 cttagtagaa aatacgtgta taacacttac tgaaatgctt agtaaaaata cttagtaaaa
     1081 tatgtgtata tagtatcata ccgacattct caacatcttc agacttccat acatactggg
     1141 tgttaatttt tcacttttac agccagcatg gagttttctg tcctaaaaac agagcagatt
     1201 tactctggaa ttatttttct gccctcgtat ttcaggcccc agaaaagaca atccgaagca
     1261 aaccgtttgt caacctccat atcatctttt acccccggaa agtgatcgcg ttttacaacc
     1321 agaatgacag ccatactgat tatgtcgtct cccggccccc ttcacatata acatgcatgt
     1381 ctgtcattgc ttactgctct acatgacagg atttcacata caggatgcgc attcagtgtt
     1441 tttatcgcca ctataacaga cagattactg tcgcttttac tcttcagatt gttaatctga
     1501 ccacaagaac agtcgaacag tacaacgaca cccccaagga gaatatgagc cgtagttgat
     1561 agcatgtttt attttattat gaataggtca agaaatagtt cttgatatgt ctggaatagt
     1621 tttgtgaata gttctagaat atccccataa tcagctttga agtgcttgca gggtgatatt
     1681 tttattgatc cccttgcata tacaactgac atgtgatggt gaaaatcacc agaaaagaaa
     1741 acgattgagg cgtaatcaga tgaaacaata taaaggcggt tataacgtga atagatatga
     1801 ctggaaaaat aaaacaactg agcatttatt gaatgataat aatctggcag ataatcacat
     1861 acctgaatgg atcagaaaaa atgaaattgc agcattctgg ttctggttaa ttatgtctca
     1921 tacagttccc gaaaaactta aacttaataa tgaatctgag ttcaactctg ccttgaaggg
     1981 ggtgatcgtt atgaaggata accccatgac aattattcct tcaagtcatg aaacacgact
     2041 gaatgttata gggaaatgga ttaatgcgtt aatcctttat ggggaagcag atcggattat
     2101 ctcaataatg catcaaaact ggctgggact cgaaaagaga aacgaagcca aatggcttaa
     2161 aaagaatacc gaacaactga aatgggcatg ggaatatatt gatgcacgta tctcacccac
     2221 atatctttcc tggtttaatc ctgtaaatga taacgaaaga tatattgcca ttgtagtttt
     2281 actaaaaatg ttattcccca cagacacacc ctgtttactg acgccaaaag aatttcacct
     2341 aggattcgct gcaaaagaaa gatttttcga caagatgcac aatgcattca gaaaacagtt
     2401 tatcgatgga aaaaaagata aacgggtaca aatcaacgta aagatatcgc catccgcaaa
     2461 aagcgctctg gacagactga ccagagagcg gaaaacaaca cagcaggcta ttctggaaca
     2521 actgattctg tacggtagac tggattaact ttcaccactt tcgctcctgg cgattttctt
     2581 ctttttgtag tgtctgcttg ctgcgggcat caggtatttt tccgtcattt tgttgcagaa
     2641 cgcttcaatg tagttaacca ttttctggtg tgctttcacc ttttcctccc caggggacat
     2701 tttcagataa atgtcctcct tttccacaag tgcatttata aactcctcta aacatccctg
     2761 gttataaagc gacgtgaaaa acaacacatt ccccagtctc tccagtaatt gcgatttaac
     2821 cctgtattgt tgttttctga gaattgattg ttttctaaag ctcagtcttg ttaaaagttg
     2881 ttcaaatctt ggttcaaatt taaaaacaga ttttaatctt agggattgat actcctgata
     2941 aaacagttca aacgtcgggg atgctgaccg aagaacaggt cgggccacag cccgtggact
     3001 aaatcccatg actgagattt cctttccctc ctcctgcaag ttcagaggca gttcagcaat
     3061 ccatgcgtcc agcttagctg aatcgctctc ctttctcaaa acctcataca aactgcccgt
     3121 gaacggttga gccgattttg gggttgcttc accttttgac tgtctgaatc catccacttt
     3181 tctccggata ctacgcggaa tcagcataaa aggcggggca ttaagagata aatgtttacc
     3241 agtcatacga tggcattgtc ccacctcgat ccaacaggaa aatttactaa ccgaccttat
     3301 cttaagcgca attatgttaa gcaggtacag ataacgcacc gtataacgat taaaatgttt
     3361 ctcaggatca aacgattctg accgttcccc tctgtacaca agtccactac gcttcagtat
     3421 tcgctgggta tctttccagg cctgactttt acaaatgctc attcggcgta gcgtattaat
     3481 cgacagtgat gccatcactc ctcccgaact ttgtcactcc agattttttc acgcccatat
     3541 tacctgaatg ataaaccgga ttagtgtgat ccggttcagt gaaatcaaca ggataccggt
     3601 atgtcattca gccattcttc cctctccgca caagtgaaat catatctgac gtttcttcct
     3661 gaagaaattc gccagaaaat ccttgaacat ctccacagtg ttattcatta cgagcccgt
//
LOCUS       DBBGLQ010000234         3698 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.234, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000234 DBBGLQ010000000
VERSION     DBBGLQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.234"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            214..567
                     /gene="yebF"
                     /locus_tag="ACIYSB_005345"
     CDS             214..567
                     /gene="yebF"
                     /locus_tag="ACIYSB_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGC5432156.1"
                     /translation="MNKALSSILLIFGICTSSLAAEKNRTVSFPRCEGQEASTVAASV
                     KRDYLQKQVLQWKDDQEILGQLRPVVWVNLREPVNKGDNVITVHLTARGKKADIQYRV
                     RVDCKNGSATWQRQG"
     gene            complement(614..1186)
                     /gene="dolP"
                     /locus_tag="ACIYSB_005346"
     CDS             complement(614..1186)
                     /gene="dolP"
                     /locus_tag="ACIYSB_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGC5432157.1"
                     /translation="MQRWFLPFLSTLLLLQGCVAAALVGTAAVGTKVSVDPRSAGTQI
                     DDEILEARVSRALSKDSQLRGDARIIVTAYQGTVLLTGQAPDRSAGEKARQIADGVEG
                     TVQVWNEIRTGKPLRADKTATDAWLTTKIRAQLLKSSRVKLSDVKVLTENGEVFLMGR
                     VTEKEGQAAADIASRVAGVKHVITAFSYIR"
     gene            1510..1803
                     /locus_tag="ACIYSB_005347"
     CDS             1510..1803
                     /locus_tag="ACIYSB_005347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432158.1"
                     /translation="MRIRVFGYRHCPSVHRIMHHATRIPDEMEPDVVFIDVAKHSVTL
                     RDMADRAGCPVDNFPCVFVDDVYLEPLHQNRANRTLHTAEYLLPDMDNRFHSG"
     gene            complement(1928..2362)
                     /locus_tag="ACIYSB_005348"
     CDS             complement(1928..2362)
                     /locus_tag="ACIYSB_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010280495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGC5432159.1"
                     /translation="MPARINMFFTRLTDYPDTGLLLLRLTFGILMLFHGVAKIEHGVN
                     WIISMLQDAGLPGFIAYGVYIGEIVAPVLIILGIFTRPAALIYALTLIVATLLVGTGK
                     FFTLTEVGAWGLENEMLYFMGGIAIMLLGPGQYSVLTRTSLR"
     gene            2948..3121
                     /locus_tag="ACIYSB_005349"
                     /pseudo
     CDS             2948..3121
                     /locus_tag="ACIYSB_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3340..>3698
                     /locus_tag="ACIYSB_005350"
     CDS             3340..>3698
                     /locus_tag="ACIYSB_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HGC5432160.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITRPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLSG
                     VPEVIVSSGVTPVGITEG"
BASE COUNT         1046 a          894 c          796 g          962 t
ORIGIN      
        1 aaaatcgatc gttaagcaga aaaaagattt atcgtcagcg aagatttgtt tatggaaatg
       61 agtgaaaaac gggttaaaaa acagactgtg ctgctcatgt gaaacgggaa gtgacgggat
      121 aagaagttat gtgaggattg ttacgggaaa tgctgtttta cgttgttaaa atgtaactcc
      181 gcaatgaaac tattccctgt ggaggtcctg taaatgaaca aagcactctc ttccattctg
      241 ctcatcttcg gtatctgtac atcatcactg gccgctgaaa aaaacagaac cgtcagtttt
      301 ccacgatgtg aagggcagga agcatcgact gttgcagcca gcgtaaaacg ggactatctc
      361 cagaaacagg tgcttcagtg gaaagatgac caggaaattc tcggtcagct gcgccccgtt
      421 gtctgggtga atcttcgtga gccggtaaat aaaggtgata atgtcatcac ggtgcatctc
      481 actgcccgtg gaaagaaagc agatattcag tatcgtgtgc gcgttgactg taagaacgga
      541 agtgctacct ggcaacgcca gggatgaacg ggtgaagttg aatacagagc ccgaacgaac
      601 aggctctcca tactcatcgt atataactga atgcggtgat gacgtgtttc acccccgcca
      661 cgcgactggc gatatcagca gcagcctgtc cttccttctc tgtaactctc cccataagaa
      721 agacttcacc attctccgtc agtaccttta catctgacag tttcaccctg ctgctcttca
      781 gtaactgtgc ccggattttt gtggtcagcc acgcatcagt agctgtttta tcagcccgaa
      841 gtggttttcc cgtccttatt tcattccata cctgcacagt cccctcaaca ccatctgcaa
      901 tctgacgggc tttttcacct gcactacggt ctggtgcctg ccctgtcagc agtacagtgc
      961 cctggtatgc tgtgacgatg attcgcgcat cccccctgag ttgtgagtcc ttcgacagcg
     1021 cacggctgac ccttgcttcc agaatttcat catcaatctg cgttcccgca cttcgggggt
     1081 ccactgacac tttagtaccc acagcagccg taccgaccag cgcagcggca acacaaccct
     1141 gtaacagcaa cagagtcgaa agaaaaggca gaaaccagcg ttgcatggca gaatcctcca
     1201 cggtctgata tccggaattt cgcagtatga cggcaggaat cagccatcac agataccaca
     1261 agcttcagag agtaacgaaa acgttaaagg aaacggaagt gcctgaatgt aaagaacggg
     1321 gaaccacaca gggtatggac aaacataata acaataacag gatgaaaata ttgaataaaa
     1381 aataaaaacc gagacatcat catttgcagt aatagccacc tccttaccga aaagtgtgag
     1441 ttgccggaca ttttttaaac gcatgataag gtctgatgat acagtcaccc tgcaaccagg
     1501 aggtacatca tgcgtatacg tgtttttggt taccggcact gcccttcagt ccatcgcatc
     1561 atgcatcacg caacgcgaat ccccgatgaa atggaaccgg atgttgtgtt tattgatgtc
     1621 gcgaagcaca gtgtgacgct gcgggatatg gctgaccgtg caggctgccc ggtggataac
     1681 ttcccgtgtg tgtttgttga tgatgtctat ctggagcccc tccaccagaa ccgggcaaac
     1741 cgcactttac atactgctga atatttatta ccggatatgg acaatcgctt tcattcggga
     1801 taaaaaatac tgcgcgttcc tggtttattt tgcgttgctg tcatgacgtg tttgccttca
     1861 ttgccgggat cgcgcacttt ttgttataac tccccggagg acactgtctg taatcagatg
     1921 gaaaccttta tctcagtgat gtcctggtta atacagaata ctgccccgga ccaagtaaca
     1981 taatggcaat cccccccatg aaatacagca tttcattttc aagcccccac gcccctactt
     2041 cagtcagtgt gaaaaacttc cctgttccga ccagaagcgt ggcaacaatc agtgtcagcg
     2101 cataaatcag agcagcaggt ctggtaaata tcccaagaat gataagtacc ggagccacga
     2161 tttcaccaat atacacacca taggcaataa atcctggcaa accagcatcc tgcaacattg
     2221 atataatcca gttgacaccg tgctctattt tggccacacc atggaacagc atcaatatac
     2281 caaatgtcag tctcagcaac agaagacctg tatccgggta gtctgtcagt ctggtaaaga
     2341 acatatttat tcttgctggc ataaaaaacc tcgttaaacg ataacagata atgaagaaac
     2401 tattatcgtt gagtcgacac catcaattga ccagatattt ctgacattca taatcaaaaa
     2461 atttgacaaa actgtaaacg ttcgcttttt ataccaacac agaatccact cagtcacaca
     2521 acctgtatgc gtcatttcct gctgttattt aaaaaatttc tcacgtaaca gacatttcaa
     2581 aaaaacacta aaaaatacca gaacaattaa gataatatta cgatcaaata agtaaaagtc
     2641 ttttgtctgt tatttttatt ttctgtgatt actgtcattc tttcaccact ttaattaaca
     2701 tcgcataata atccgggagg tggataagat atgaaaatta actatccttt actggcgctg
     2761 gcgttgaatt attacctgta gagctcactc cggatgagca gaaagaaccg atggtggctc
     2821 ttactccact tttatccact ccttcacaca gcactgtcag tgcaggtgcc tgaaagatct
     2881 cgttccgtca cggtaaaatg acgctggaaa attcttcacc agaactgctc actgtgttaa
     2941 ttcgtgaact gacctggggg gaatgcgtgg taccctgctg tacggactaa tcggaacctg
     3001 tcggttgaac ggtatcgatc cggaagcgta tctgcgccat atcctgaacg ttctaccgga
     3061 atggccttcc tgcatggttg atgaactcct gccatggaat atggtgctcc cattggggta
     3121 agcgtcaata cggcgttcgc ttaacttaac acttactttc aaagcctgat atctgtccta
     3181 cgcttttcct caataccaca tcgccagcag gaaacaacct aatgcctgtg ttggggtctg
     3241 aattactaat gataatcatt tgcattgtgt atagcttatg ctataaaaca tagccgttgc
     3301 tatgtttgtt ctaattttac tcactatagg tactgaatta tgcactcaat aaaaaaagta
     3361 accatgctcc tggggtgcct cgccctcacc tgctcgatag catttcaggc gagtgcaact
     3421 gaaaaattca aggtcattac aacatttacc atcatcgcag atatggccaa aaacgtggct
     3481 ggagatgctg cagaagtctc atccataacc aggcccggtg cagaaattca tgagtatcag
     3541 cctacccctg gcgatattaa acgtgcgcag ggggcacaac tgattctcgc caacggtatg
     3601 aatctggaat tgtggttcca gcgcttttac cagcatctca gtggggttcc agaagtgatt
     3661 gtctcttcgg gtgtgacgcc agtagggatc accgaagg
//
LOCUS       DBBGLQ010000235         3650 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.235, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000235 DBBGLQ010000000
VERSION     DBBGLQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.235"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..204
                     /locus_tag="ACIYSB_005351"
     CDS             <1..204
                     /locus_tag="ACIYSB_005351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432161.1"
                     /translation="NTQQRHKWFLKTTKPEGKRINRTCKQNIKNAIRTLSGAVNQQQH
                     QNLQLNHTTQSQSRIAHLKPVYR"
     gene            complement(1364..2494)
                     /locus_tag="ACIYSB_005352"
     CDS             complement(1364..2494)
                     /locus_tag="ACIYSB_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432162.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLAELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDVLSELYAIYSWVSYCKRSDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYSANSSGVSGHNDSKSLYKTVLSVC
                     GGKRETELFFYSLPVKTNHHPYWIKSGVVKRFPPVFGLLFYEQERLTGGVFRYVLIRF
                     YNTFKENLFFVEIVSCCFLVELFHDIDSFFINQRLSKLESFIDKWREGKEIWIKAYLQ
                     LIHNTLGITETEKLVNLYNSFFTDKVVIWDESVKKVKWIYEK"
     gene            2679..3563
                     /locus_tag="ACIYSB_005353"
     CDS             2679..3563
                     /locus_tag="ACIYSB_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGC5432163.1"
                     /translation="MSFSHFSLSAQVKSYLTFLPEEIRQKILEHLHCVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLQVLVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGDAIGKSFDDAAARFSFPAWMLQLLR
                     KARARIIHLLVTLWDHLF"
BASE COUNT         1127 a          865 c          600 g         1058 t
ORIGIN      
        1 aacacacaac aacggcataa atggttttta aaaactacaa agcctgaagg gaaacggatc
       61 aacagaacgt gcaaacaaaa catcaaaaac gcaatcagaa cgctttctgg cgctgtaaat
      121 caacaacaac accaaaacct acagcttaac cacacaactc aatcacagag ccgaatagcg
      181 cacttaaaac ctgtttacag ataaccctgc cacaagaaca aagttcacca atatcagaat
      241 cgattttctt atcctgaaaa cgttactttt caacacacca taaaaacgat taaccatcga
      301 taacgtaaat ctcatctgaa tcaaaacacc agaaccaatc atcacgcttt ttctgaaaag
      361 agccttatga tgaagataat caccttgcac tgagaaaaaa caccagggcc agttaatcat
      421 ctcatcaatc tcatcgtcac aaaatcatcc atagaaccat ttaacaaaat cgtcgcaagc
      481 acaccgtaaa tcacaccagg ttatctcagt cggatttgcg tttttacgta aagacacttg
      541 tctcaggtta acgactaaaa acacaaaaac tcagaaaagg ttaaacgcaa catcagtaaa
      601 atcgaatagc agattgatta cttaaacaga aagattttac agcagcttta aactgatttt
      661 agctaacgta aagatttact aaactggatg tgtatcagac aaagcaatgt gtatagcaat
      721 acttaatgaa aacacttgta gggttgttta tgaaaacacg tgtatgtctg gtaaaacact
      781 tactgaaatg cttagtaaaa atacttagta aaatacgtgt atatagtatc ataccgacat
      841 tagcaacatt ctcagacact catgcagact gaatgttgat tacccgtttt cacatcaggc
      901 atggttcttt actgaccaaa aaacaaactg gtttactctt gaattatttt ctctacctca
      961 gaaaacatgg tctcaggaat ctgctatggt ttcatagttt ctgttgttct tttcataaaa
     1021 gttataagtc attgtcataa tcatacgata atcactttat gttagttttt actgtcacac
     1081 gctactgact ttttcagtca ttgccagaaa ggggctcttt ttacttccat ttcaccactc
     1141 aacaaatgca aaacttgtcc agtaaagcgg accacctctt gctcacaagc aacgaatcat
     1201 ctccaggaac agccctcaaa cattgcagaa acaaaaacaa caaaaccctc attatcactg
     1261 atgttcggaa aatttcccga aacaaaaaaa acgaaatcaa aatgagactc attatcatgg
     1321 atatagtcaa aaagacgtta tggtggcttt cagttaaatg gtactatttc tcataaatcc
     1381 acttaacttt ttttactgat tcatcccaga taaccacttt atcagtaaaa aatgaattat
     1441 atagattgac gagtttttct gtttcagtta ttcccaatgt attatgtatc agttgtagat
     1501 atgcttttat ccagatttct ttaccctccc tccacttatc tataaaactt tccagcttac
     1561 ttaatcgttg atttataaaa aacgaatcaa tatcatgaaa aagctcaaca agaaaacagc
     1621 acgagacaat ctcaacaaaa aacaaattct ccttaaaagt attataaaac cttatcaaca
     1681 cataacgaaa aacacctcct gttaatcttt cctgttcata aaacaacaaa ccaaaaacag
     1741 gaggaaatct tttaaccaca ccacttttaa tccagtaagg atgatgatta gtctttactg
     1801 gcagtgaata aaagaacaat tcggtttctc ttttcccacc acaaacagac aaaactgtct
     1861 tataaagact ttttgaatca ttatgtcctg ataccccaga cgaattagca gagtattttc
     1921 tttttacttt ttgccttaat ggagctggaa gaataataaa acgggatggc agataacaat
     1981 cgctttcaga agaccggcat tcgcacacat gaagatttat atttacatcc ggacaattca
     2041 aaaaccacac agaataatta tcgctccgtt tgcaatatga aacccatgaa taaattgcat
     2101 aaagttcaga aagtacatct aactctgctt ttgatttaaa tgtgataccg tttcttcgaa
     2161 ttctcgtttc cagcgttctc catgccggag ttttcagtat ctcacgaact tttttattaa
     2221 gttcagccag cgatggtccc tgctcctgtt tatgtttctt cctgctgtta atgatttctc
     2281 tttcttttac taattttttc ttacttttgg gtccttttat ctttcttggc aatgcttcat
     2341 ttaatctgtg aacaggattt tgctctcttc tttttttgag ctcacaaaac ctctcataat
     2401 gttttctgca cagtcttttc tccactgcca tttttactga gaaaacctga ttgttacaat
     2461 tttttctgac gcatttgttt ttacgtccgg tcatcctctc atctcctgaa accgcagtat
     2521 gtcattagtt cccgttcgga aaaactcact tcatactcca ttacacttta acgatcatgt
     2581 ctgtaattca gacattccat catttttcac gtccatatta catgaatgat aaaccggatt
     2641 agtgtgatcc ggttcagcga aatcaacagg ataacggtat gtcattcagc catttttccc
     2701 tctccgcaca agtaaaatca tatctgacgt ttcttcctga agaaattcgc cagaaaatcc
     2761 ttgaacatct ccactgtgtt attcattacg agcccgtgat tggcattatg ggtaaatccg
     2821 gtacaggcaa aagcagtctg tgtaatgcca tttttcagtc ccgtatctgc gccacacatc
     2881 ctctgaacgg ctgcacccgc caggctcatc gtcttaccct gcaactcggt gaacgcagaa
     2941 tgacgctggt cgatctgccc ggcattggtg aaacaccgca gcatgatcag gaataccggg
     3001 agctttaccg tcagttactg ccggaactgg atctgattat ctggatcctg cgggctgatg
     3061 aacgtgcgta tgctgctgat attgccatgc atcagttttt actgaatgaa ggtgcagatc
     3121 cctcgcgttt tctgtttgtt ctcagccatg ccgatcgcgt gtttcctgct gaagaatgga
     3181 acgacacaga aaaatgcccg tcccgtcagc aggaactctc actggcgaca gtaacagccc
     3241 gggtggccac cctgttcccg tcatcatttc cggtactctc cgttgcagcg cctgtcggct
     3301 ggaatcttca ggtactggtg tcactgatga tccacgcact gccaccacag gcaaccagcg
     3361 cggtttattc acatatccgg ggggaaaacc gctctgaaca ggcccagaaa cacgcacaac
     3421 agacttttgg tgacgccatc gggaaaagtt ttgacgacgc cgctgcccgg ttcagttttc
     3481 cggcctggat gttacagctt ctgcgtaaag cccgggcccg cattatccac ctgctggtca
     3541 cactgtggga tcacctgttc tgagcccccc ggagtccccc gtggcaaccg tataccttta
     3601 cacgacaacg gattacggta accactgccg tataccgtct gtcattatca
//
LOCUS       DBBGLQ010000236         3647 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.236, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000236 DBBGLQ010000000
VERSION     DBBGLQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.236"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..614)
                     /locus_tag="ACIYSB_005354"
     CDS             complement(<1..614)
                     /locus_tag="ACIYSB_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGC5432164.1"
                     /translation="MRLTSRFGYVNQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRTGEINGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGNEHQPVTTADILT"
     gene            complement(953..3466)
                     /gene="agn43"
                     /locus_tag="ACIYSB_005355"
                     /gene_synonym="flu"
                     /pseudo
     CDS             complement(953..3466)
                     /gene="agn43"
                     /locus_tag="ACIYSB_005355"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
BASE COUNT          729 a         1067 c         1005 g          846 t
ORIGIN      
        1 gtcagaatgt cggcggtggt gacgggctga tgttcgttac cataacggta agtcagcgcc
       61 gcctgtgcca gcgcctggcg tgccggtggc ggcaggacca gcgactgcat ggcatcacgc
      121 ttctcctcta tccggtcaaa cacgccgacc acttcgtaag caccttctat gaccctgtcc
      181 accacgtttc cccggtgtgg cacccggact tctcccagcg actgaccgca gaccaggcca
      241 ttggtacaaa tggcacgaaa atatccgggt aacatctggt agctggatga accgtcgtgg
      301 gagttgagca gaataatttc agggacatgt tgtccgttta tctctccggt acgccgcaga
      361 cgcagcatgt gtttggtata cccccggcgg cccgggtcac gtacgcgggt ctggcaggca
      421 aagaacggct gaaagccttc ccgctgcagg ctttccagta cggtgatggt ggggatgtac
      481 gtatagttct ggcttcggga cgtgtgttta tcttccccga aaatccccgg cacatggtgc
      541 atcagttctt cgtgtgtcag cggacggtca cggcgtatct ggtttacata accaaaacga
      601 ctggttaatc gcatgatttg ctccttatcg gtggttaaga tttactggtg taataaatga
      661 aaaaagccac gtctccctga gaagacgcgg cctgattggg gaaatgaatg acgtttattg
      721 tctgagaagc cctttactgg cgagcagtgt gttttcatgg ctggagaacg gccagtaacc
      781 ccatcgggat tgggttataa ccgaccagac tgtaaaccat tcagatgcca ttttgcagca
      841 aaagggcttc cggacaacag acgtgatgta cagccccggc atatctgttg ttccctgaaa
      901 gtaagaagcc ccggtcatga tgaccggggc ctgaaggaga gtgacctgat tatcagaaag
      961 tcacattcag cgtggcctga ccgttatagc cttcagcgct gctgccgctg acgctgtggg
     1021 cataaccggc ctgaaccccc agggtgatat tttcccggac acgggcttcc agtccggcct
     1081 gcaggtccag tgacgtgcca ttccgggacg gtgagaacgt catgttactg ccggctgtgg
     1141 ctgtccccgt gctcatgtct ccccgggagc tgaaggtgcg gataacagaa ggctgtaccc
     1201 acccgttcac cggcagttca cgcacactgt gtctggcact gtcgcgcagg gtgtcacggg
     1261 atgaggtgcc tttaccagag ctcatatcgt tgtggctgcc cagacggaaa ccggcacgca
     1321 catgttgtgc actgccatgc ccgaacttca cataaccggc gctatcccgg ccgtcatcca
     1381 gggaaagtcc ctgccaggta tactgcagtt gtggctccag catcaggttg tcagtgatac
     1441 tgaagggcag accggtttcc agcgaaccca gccagccccg gccccgggcg cggaagtcgt
     1501 tattgtccga tgacgctttc atgctgtggc gggttccctg ggccacaatg tcagcccata
     1561 ggccggagga tgtgtgtacc agattcaggt atccgcccag actgccggca tcatcccgga
     1621 cagtgccggc gcgggagccg tcatcatcct taacatcaac ggaagaatgg cctgcagcac
     1681 catatacccc cgctgtcaca gacataccgg caacctctgt tctgagcagg tccccctcca
     1741 gacggacgaa gccatagctg ccgctgcttt ccggcgtgac cccacgggca ataccgccgt
     1801 tgttatcgtg cccgagatga ccgccctgaa tgctgagacg gacgctgttg ttttcaccat
     1861 ttacaccggt ctgatggctg cgggagcctg ccagaatccg gtcatagtcc attgcctgtg
     1921 tcagcatgga ggcatacaag gggacttctg cacgataaac attttcactg cgcagatacc
     1981 agctctcatc actgtcccgg ttgagggagt agttaaaggc cccggcctgc agcctgttcc
     2041 cctggacaaa ggccccttcc tctgtggtgg caccgttaat ggcttccacc acccgaatac
     2101 ccttaccggt ggtcgccagc cccgacgcac tgttgccggc gttcaccagg ttcaggatgg
     2161 tttttccggt tgccctgcca ccgtcaatga ccagtctgtc agcattgttc tgtgccatat
     2221 ccgggcgtac acgcaggctg atggtgccat tctgcccgtt caggtttttc actttcaggg
     2281 tcgccggtac gaacttccct gtgcgggtgg aggtgaaatg aatctgtccg gcatggctga
     2341 ggtcatccac caccgactgc accgtggcgt tatcggggat attccaggtg gcaccggagg
     2401 cgagagtgac attcgtgggg tcaatggcac cgttcagcac cgtgctgccg gtcagcttca
     2461 gggctgtacc gcgctgagca gtgacatccg tggtgacggt actgttgttc agcgtcagcg
     2521 tgccttcatt caggttgacg gctttctggg tgagagtggt gttgctgaca gtgagtgtgc
     2581 cacttcctga tttttccacg ctgccgttac cggtgagagc gcctccctgc aggagtgcat
     2641 cgccttcacg gatggtcagg gtatcgttat tcaccgtttt cccggaaagg gtgagcgtgg
     2701 ctccgttatt cagcgtggtg gtaccttcca ggattccgcc atgtgcagtc agttgtccgc
     2761 catttaccgt ggtatccgtg gctgtatcac cggcgttcag cgtgaatgca ctgccttttc
     2821 ccagcatcag ggcatccgcc tgaccacccc cgatacggaa tgccgttccg tcgctccggg
     2881 taccactgac agcggcaccg gaatcggcca gcagtacacc gccatccccc atggatatgg
     2941 tggtggctgt gccaccactg cggacatcca gggtaccccc ctcatccaca tgtgtgtttg
     3001 tggcggtatg gccgctcagt acatccagac gtccgccatt ttccagaacg acattatcag
     3061 ctttcccctc cacaacagag aatacgccca gacggtttgt gcctgtaacg gtagctgctg
     3121 tactggtaac cagtgcgctg cccgtgttct gggtgacatc agtcgcttta ccaccggcgt
     3181 tcacctgcag tctgcccttc tgattaacgg tggtattctc agccaccgcc ccttccttca
     3241 ccacctgcca gccaccgtcg ttaatcaccg tattcagtgc cagcccgtcc ttatgcacat
     3301 actggttccc tccctccagc cgggtattat gtgcctctcc gtgcaccgtc tggtcaccac
     3361 ctgcgtaaat gagggtgtca cgggccagtc ctgaagacca gataacctgc cggccatttt
     3421 tgttgatggt ggtggattca gccgtccccc cgaccatctg accggtggag acatttgtaa
     3481 gcgtaaactg accgccgtat gtagccatca gacgagaatt ggtaacttag acgcccatct
     3541 gatatagacg gacatctaag tatggaatta caggactggc gaaaagaacc tcgtaaaaac
     3601 tattcgaatg aattcaaact tcgtatggtg gaactggcat cacaacc
//
LOCUS       DBBGLQ010000237         3626 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.237, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000237 DBBGLQ010000000
VERSION     DBBGLQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.237"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..950
                     /locus_tag="ACIYSB_005356"
     CDS             <1..950
                     /locus_tag="ACIYSB_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGC5432165.1"
                     /translation="NGSVPNDELFGIIYTVDEGDNWTDPQVLEKANPNIGVSVYREFL
                     LSQQQRAKNNARLANVFKTKHLNIWVSARSAYFNLVSWQSCEDKSLTLEQFEGQPCIL
                     AFDLARKLDMNSMARLYTREIDGKTHYYSVAPRFWVPYDTVYSVEKNEDRRTAERFQK
                     WVEMGVLTVTDGAEVDYRYILEEAKAANKISPVSESPIDPFGATGLSHDLADEDLNPI
                     TIIQNYTNMSDPMKELEAAIESGRFHHDGNPIMTWCIGNVVGKTIPGNDDVVKPVKEQ
                     AENKIDGAVALIMAVGRAMLYGKEDTLSDHIESYGIRSL"
     gene            962..1144
                     /gene="ymfR"
                     /locus_tag="ACIYSB_005357"
     CDS             962..1144
                     /gene="ymfR"
                     /locus_tag="ACIYSB_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HGC5432166.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            1144..2385
                     /locus_tag="ACIYSB_005358"
     CDS             1144..2385
                     /locus_tag="ACIYSB_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGC5432167.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELAEAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPVAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            2363..3013
                     /locus_tag="ACIYSB_005359"
     CDS             2363..3013
                     /locus_tag="ACIYSB_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGC5432168.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            3028..>3626
                     /locus_tag="ACIYSB_005360"
     CDS             3028..>3626
                     /locus_tag="ACIYSB_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGC5432169.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEELDRNILNLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKML"
BASE COUNT          877 a          898 c         1096 g          755 t
ORIGIN      
        1 tcaacgggtc ggtacccaac gatgaactgt tcgggatcat ctataccgtt gacgaaggcg
       61 ataactggac cgacccgcag gtgctggaaa aagctaaccc gaatattggc gtgtcggttt
      121 atcgcgaatt tttgttaagt cagcagcagc gtgcgaaaaa taacgcccgt ctggcaaacg
      181 tctttaaaac aaaacacctc aatatctggg tgtcggcgcg ttcggcgtat ttcaacctgg
      241 tgagctggca gagctgcgag gataaatcac tgacccttga gcagttcgag gggcagccgt
      301 gcattctggc ctttgacctg gcgcgtaagc tggatatgaa cagcatggcg cgactttata
      361 cccgcgagat tgacggtaaa acgcattact acagtgtggc cccgcgtttc tgggtaccgt
      421 atgacacggt gtacagcgtc gagaaaaatg aagatcgccg gacagccgaa cgctttcaga
      481 aatgggtgga aatgggcgtt ctgaccgtta ccgatggtgc ggaggtggat tatcgctaca
      541 tcctcgagga ggccaaagcg gcgaacaaaa tcagcccggt cagtgagtca cccatcgacc
      601 ccttcggggc gaccgggttg tcacatgacc ttgctgatga agacctgaac cccatcacta
      661 tcattcagaa ctacaccaac atgtccgacc cgatgaaaga gctggaagcg gcaattgaat
      721 cggggcgctt tcatcatgat ggcaatccca tcatgacctg gtgtatcggc aacgtggtcg
      781 gcaaaaccat tccgggtaac gatgatgtgg tgaagcccgt caaagagcag gcggaaaaca
      841 aaatcgatgg tgcagtggcg ctgattatgg cggttggcag agccatgctg tacgggaaag
      901 aagacacgct gtctgaccac attgagtcct atgggatccg ctcgctttaa ctgaggtaat
      961 tatgatcatg ctgattctcg cgcctctggt gggcgtgctg ggggcgcttt tgctggcgta
     1021 tggtgcctgg ctgatttatc ccccggcggg gtttgttgtt gccggggcgt tgtgcctgtt
     1081 ctggtcgtgg ctggtggcgc gatatctcga ccgtacacag tcgtctgtcg gcggaggtaa
     1141 atagtgttct tttcgggatt atttcaacga aaaagtgacg caccggtgac cacgccagca
     1201 gagctggcgg aggctatcgg gttgtcctac gacacctata ccggaaagca gatcagcagc
     1261 cagcgggcca tgcgactgac ggcggttttt tcctgtgtca gggtgctggc ggagtcggtc
     1321 gggatgttgc cctgcaacct gtatcacctg aacggcagcc tgaagcagag agccactggc
     1381 gaacgtctgc ataagctgat ctccacgcat cccaatggct atatgacgcc gcaggagttc
     1441 tgggagctgg tggtcacctg tctgtgcctg cggggaaact tttacgccta caaagtgaaa
     1501 gcatttggcg aagtggctga actgctgccc gtcgatcccg gctgtgtggt accgaagctt
     1561 aacagtagct gggagccggt ctatcaggtc acattcccgg atggctccac ggatgtactg
     1621 agccaggagg atatctggca tgtgcgcacg ctgacgctgg acggactggt ggggctgaat
     1681 cctgtcgcct atgcccgcga ggcaatatcg ctggcggcag cgaccgaaga gcacggggcc
     1741 agactgttca gcaatggtgc ggtgacgtcg ggtgtgttgc gtacagagca gacgctgtca
     1801 gatcaggctt acgagcgcct gaagaaagat tttgaggagc gtcacaccgg gcttggcaat
     1861 gctcaccgcc cgatgatcct tgagatgggg ctggactgga agtcgatggc gctgaacgcc
     1921 gaggacagcc agttcctgga aacccgcaag tttcagcttg aagaaatctg tcgtctgttc
     1981 cgggtgccgt tgcacatggt gcagaacacc gatcgcgcca ccttcaacaa tatcgaagag
     2041 ctggggctgg gatttatcaa ctattcactg gtgccgtatc tgacccgcat cgaacagcgg
     2101 atcaacaccg gactggtacg aaaaagtaag cagggcgttt attacgccaa atttaacgcc
     2161 ggggcgttac tgcgcgggga tatgaagtcc cgttttgaag cctacgccac cgggatcaac
     2221 tggggaattt actctcccaa tgactgccgc gacctggaag atatgaatcc gcgtcccggt
     2281 ggtgatgtct atctcacacc gatgaacatg accacgaaac cctccgatgg cagtaaagcc
     2341 ggtaagcaga aggataacgc caatgcagac gaaacaacgt cttgatgtac cgctgagtct
     2401 gaaatctgtc agtgactccg gtgagtttga agggtatggc tccgtctttg gtgtaaagga
     2461 cagccacgat gatgtggtga tgtccggggc atttgctgct tccctgcggg cgtggagtga
     2521 cagaaaagcg ttacctgcgc tgctctggca gcaccgcatg gatgaaccca tcggtgttta
     2581 caccgaaatg aaggaagacg atgtcgggct ttacgtcagg ggacggttgc ttattgatga
     2641 tgatcccctc gcaaaacgcg cacatgcaca catgaaggcc ggttcgttaa ccggcctttc
     2701 tattgggtac gtcctgaaag actgggaata cgaccggagc aaagaagcct ttctgctgaa
     2761 agaaatcgac ctctgggaag tcagcctggt gacgttcccg tctaacgacg aggcgcggat
     2821 cagcgacgtc aagaacgcac tggcccgcgg ggaaatcccc gaacagaaaa aaatcgaaag
     2881 agtcctgcgt gatgtcggac tctcccgtac ccaggccaaa gcattcatgg ccgggggcta
     2941 tggcgcactg tccctgcgcg acgctgagga tgtgggctct gcactgaatg cactgaaaaa
     3001 tctgaacttc taatcaggag aaatacgatg gcggttgata ttaaagatgt cgaacaggtc
     3061 gcgcaggagc tgcagcagaa gtttgacgac ttcaaagcaa agaacgacaa gcgcgtggat
     3121 gcgattgagc aggaaaaagg caagcttgcc gggcaggtgg aaaccctgaa cgggaaactc
     3181 agcgagctgg aaaatctcaa aagcgacctt gaaaaagagc tgcttgagct gaaacgtccg
     3241 gcaggtggag cgcaaaataa actggccacc gagcataaag aggcgtttgt gggcttcctg
     3301 cgtaaaggcc gtgaagacgg tctgcgcgat ctggagcgta aggcattgca ggtgggtacc
     3361 gatgaagacg gtggctacgc cgtgccggaa gaactggatc gcaacattct taacctgctg
     3421 aaagatgaag tggtgatgcg tcaggaagcc acggtgatca ccgttggcgg ttccgactac
     3481 aaaaaactgg tgaatctggg cggtacggct tccggatggg tgggggaaac ggatacgcga
     3541 tcccagactg ccacctccag actggagctg attgaacctc tcatggggga aatctacggc
     3601 aacccgcagg ctacccagaa aatgct
//
LOCUS       DBBGLQ010000238         3532 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.238, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000238 DBBGLQ010000000
VERSION     DBBGLQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.238"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(18..1376)
                     /locus_tag="ACIYSB_005361"
     CDS             complement(18..1376)
                     /locus_tag="ACIYSB_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HGC5432170.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKVIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIAAVQSTLDIDAKSKEKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQRILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1897..2487)
                     /locus_tag="ACIYSB_005362"
     CDS             complement(1897..2487)
                     /locus_tag="ACIYSB_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432171.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSREA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQYRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
     gene            complement(2484..2744)
                     /locus_tag="ACIYSB_005363"
     CDS             complement(2484..2744)
                     /locus_tag="ACIYSB_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432172.1"
                     /translation="MDEERVFSLSYEQLTRFAEQRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            3073..3525
                     /locus_tag="ACIYSB_005364"
     CDS             3073..3525
                     /locus_tag="ACIYSB_005364"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGC5432173.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISITLGD
                     SVTVGSGAIILSPYICSGAVIGAGSVVVKPIENKGIYAGNPARLLRIL"
BASE COUNT          809 a          929 c          875 g          919 t
ORIGIN      
        1 gtccgtacct tataaactta tttcaacgct tccggcagcg tcacaatcca cagttcactg
       61 tcagactccg gtttctccag tggcttcact ctgagggtcg tttcaacctg cttgtcgtca
      121 agcgtcagct ttccttccgc gttcacccga taatccttga atgtcttgcc ctcaaccgga
      181 tgttgcatcg ccaccttcac gccaaagtcg ttatcgttcg ccaccgccag cgttttgctg
      241 tcgatcagcg ccaacccttc agccttctcc tgctgccagc ccaaggagcg cagatcaaca
      301 acctgcgttt tctgcgcaag ggtaatgcct cgctgtgaca gggttttctc atcatcaaac
      361 tccggatact cacccggctt gtcgaagcct gacaggtcgc tcgccttatt gagatcgacc
      421 tcgtaaatca ggttacgcat tctgttgttt ttatctcttc cctgttcgat cagcaggatg
      481 cgctggttat cgagcgccac gatgtctccg attttagcgt cgctgttttt gctgtaggcc
      541 gcgctgtcga taggataacc gtacatcgcg gtttttccgc ttgccggatc gaagctcacc
      601 agacgcgtaa acagcgcctt ttccttgctc ttagcgtcga tatccagcgt actttgcacg
      661 gcggcaataa tacgcccgtc cggcatacgg gtgaggcctt caaagccgcg gttcgcctga
      721 cgccatttga ggatatttgg caaaccgccc gcaatcactt tttccccttc cgccgcctgc
      781 ggaccgtgga ttgccaggat tttcccttta ctgtcgatgt taatcaggaa cgggccatac
      841 tcatcgcaca gccagtaacc tcctttacca tccggagtga tcccttccgt atccagcccg
      901 cgattatccc ccttcagtct atgtaacgtg tcgctgaagg ccacttcatt ggtggatcca
      961 atcacgtcac ttgccagcgg cagaccgttg atcgcgcctt tatcgtcatg cagaggacgg
     1021 ggatcgatgg cctccgcttt gccgttttgt acacgaatcg tcatcagcag cggcgcgaaa
     1081 tctggcgtaa cgaaaatttt agtttcgttt ttcccctctt tcggtgaatc cgcgttcgga
     1141 ccgcgatcgg taatggttgc gaatgtcagg gcatcgccct gtttaccggt gaacagcagg
     1201 ccagaaccta tcccaaccgg aaggccgttc gggaatgcac tagcaaatgc gccggcgtag
     1261 ttcacgtgcg tgccttctgg aaaactgacg acatagcgct cagccgtagg ttgtgccgcc
     1321 agggcagaga aagagagcgt acaaccgatt agaacaggaa taatttttct tttcatattg
     1381 ttagcatcca taaagtgaag atcacactac accataaaga agaaacgagt caggaaaatg
     1441 acatcgaatg gcaacatgcg cgggcctgag gtcaaagaaa tataaaacac tgttgttatt
     1501 tgtaacaacg gaaatatatt tgtctttctt cgtgatcaaa aatgctgtgt cttgttggat
     1561 tggagtgatg agggctgcgt ttaggaaacg ccctatattg tacggttgca ttatcgcggt
     1621 aggggtaatg cacgtgtcag tatattccta cggcatttca tcataaaaaa acaaccgtcc
     1681 gaactgggac ggaatgtact gaactgaaca aaggtcgtta tacaagtaac aataagccag
     1741 gccaccagac tgcttggata taaaactgat gtcgggctac atcaccggat aagaaaactc
     1801 gaaattcctg acggaagcga catatcacta cttgtaaagc taagacgctc aaagaattaa
     1861 ttgcaaagtt tcaatttcac ggagcgggaa atactgttac ggggtgacgc tttcattaac
     1921 ctgtacacga acctccattc cggcatcgtg acaggcctgc agccactgcg ccacttccag
     1981 cggatcgccc tcccgccgta ccaccctgcc ttctttattc cataactgca gacaggtgct
     2041 gccgtcgaga cgtaccacaa aatccccgcg gcaggcctga tacgtcatgc cgttcagctc
     2101 caccgccaga tcagtggcag aagtgccgtc tgcgggcaga cctgctgccg ggaccagcgg
     2161 cagtacgacg acttctgccg cacgctgttc tgcccggagg cggtactgtt ctgccagcag
     2221 cagaggctgt gccacggccc ggccggcgtc cgtcagctcc actgccagct gcaggttcgg
     2281 agcgcgcagg gtacgcagcc agccggcatc ctccagacga cggcaggagg cgcgcaggtt
     2341 tggcccgtag accggggctt ccctgcttcg ctccagcacc cgttctatgt cccgcgtcag
     2401 cacggggcct ggccgttttt catcaagggc agccagcaca atcagcactc tccgttgcag
     2461 tggcgacggt cggcggctct gggtcatttc cagtcatcct ccggccagat aagctttctg
     2521 aggcgctgat ggtcggcgtc aatgatctcc tgtcttttta tggcattcat tgaggtatag
     2581 ccatttatgg ccagttcatg ccagaagatc agcacggctc cggctttggc tgactcgcag
     2641 agataaatcg cgccctggct gtccagattg cattcccgga tgcgttgttc cgcaaaccgg
     2701 gtcagttgtt catagctgag tgaaaatact ctttcttcat ccatcctgcg cgctccatct
     2761 gataatgttc tgacaggtaa aaatcaaatt ctgtatgatt tttgtgttat cgatttatga
     2821 taatatcaga tattttttca ttacttatga ttaaaatcat atctgatatg aatttatgtg
     2881 tattgcttaa ttatcatatc acctgcccgt ttcccgccgc gcagatccgg tttccggctg
     2941 aagtggatcc agacctgatt tgttgctgag tatcggtaaa agtgcggctt ataagctatt
     3001 ctgaaacatg gcaagtgaat ctgggctgta taattccggc aagtgtcatc tgtatttaaa
     3061 ggacgtgtga gtatgaaatt tatggaatgt gctgtccggg atgtgattta tgggaccaat
     3121 gtccgtattg tcaaaccggt caacatttat gaatgtgaac tcagggacaa cgtgtttgtc
     3181 ggccccttcg ttgagataca gaaaggctgt gtcattggta gtggaagccg gatacagtcg
     3241 cacacgttta tttgtgagaa cgtaacactg ggggagaact gcttcattgg tcataacgtg
     3301 acgttcgcca acgatctgtt tcgttcagga gcaccagatc cgtcaccgga taactggatc
     3361 agcattaccc tgggggattc ggttactgtt ggcagtggag ccattattct gtctccgtat
     3421 atatgcagcg gagctgttat tggtgcgggt tcagttgtgg taaaaccgat tgagaacaaa
     3481 ggcatttatg caggaaaccc ggcaagactg ctcagaatat tatgaaaaag aa
//
LOCUS       DBBGLQ010000239         3527 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.239, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000239 DBBGLQ010000000
VERSION     DBBGLQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.239"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..83)
                     /locus_tag="ACIYSB_005365"
     CDS             complement(<1..83)
                     /locus_tag="ACIYSB_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HGC5432174.1"
                     /translation="MHDNHETQKINQTSVMPEKTGVYWNSR"
     gene            172..1269
                     /locus_tag="ACIYSB_005366"
     CDS             172..1269
                     /locus_tag="ACIYSB_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5432175.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPQKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQRSRNKTP"
     gene            1331..1579
                     /locus_tag="ACIYSB_005367"
     CDS             1331..1579
                     /locus_tag="ACIYSB_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGC5432176.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            1888..2892
                     /locus_tag="ACIYSB_005368"
     CDS             1888..2892
                     /locus_tag="ACIYSB_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SppA protein"
                     /protein_id="HGC5432177.1"
                     /translation="MQDTDVYVYIGDVHRSGHHDITKAIKERKTNHGLQKNVLFCVAT
                     YGGDPSAGYRIGRALQHNYEHITVLVVGPCKSAGTLMAVAAHKLVIGDMGELGPLDIQ
                     LKKNDEIGELSSGLVIATAIDEIKECAISTFRSYVLDIKYRNQISTKMSADIAAKLTE
                     AIISPMASQIDPIKLGEHQRAMNIALSYGERLNSHSKNLKESSLGKLIAGYPAHGFVI
                     DRKEARELFNHVESPTDFAANLYEVLGEMISSGEISIFDKPMIKDLTPKKQYETTNAE
                     EDSHDNSRIQEGDEISDQNSGRAEPPRDEPVQGGDGKHAETEEEAGTLPAEQPDEGTQ
                     "
     gene            complement(2943..3116)
                     /locus_tag="ACIYSB_005369"
                     /pseudo
     CDS             complement(2943..3116)
                     /locus_tag="ACIYSB_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461173.3"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
     gene            complement(3118..3462)
                     /locus_tag="ACIYSB_005370"
                     /pseudo
     CDS             complement(3118..3462)
                     /locus_tag="ACIYSB_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1092 a          770 c          756 g          909 t
ORIGIN      
        1 aaccgagaat tccagtaaac accagttttt tcaggcatca cgctggtttg attaattttt
       61 tgtgtttcat gattatcgtg catttttgaa catttcaggc tatttttctc gcgttaggtt
      121 cccgcacagg ttcccacgtt ttatgggaac ccgaaataac gaggtcgtgt aatggcgtac
      181 tataacatag agaaacgact aaaatccgat ggcacaccac gctatcgctg taatgtgatt
      241 atcaaagaaa aaggtgttat cacttacagg gaaagcaaaa cattccctaa acatgctcat
      301 gccaaaacat ggggcacaca gaaagtgatg gaattagatc tatatggcat tccatcatca
      361 aatgcagttg acggacttac agtccgtgac ttactacaca aatatttaaa tgacccaaat
      421 gccggaggta aagcaggccg tactaaaaga tatgtgctag aactgcttat ggatagtgac
      481 atctccgcga tcaaactatc tgaactgaca gaaaatgacg taattgaaca ttgcaggcta
      541 agaaacaacg ctggcgcagg tccagcaaca gtcagccacg atgttagtta tcttggcagt
      601 gttctggatg cggccaaacc tgtatacgga attaattaca catcaaaccc ggcgaaaagt
      661 gctcgtccat atctacttaa acttggtttg attggtaaat caaaccgtcg taatcgtaga
      721 ccagcatctg atgaactgga catgctcatt gaaggccttc aacaacgatc tactcataaa
      781 tgctcaaaaa ttccgttcgt tgatatcctc aaattttctg tgtggtcctg tatgcgaatc
      841 ggagaagtat gccggttacg atgggaagat ctcgaccagg aacaaaaatc tatactcgta
      901 agagacagga aagatccacg caaaaaggaa ggcaaccaca tgaaagtagc cttgcttggg
      961 gaagcctggg atatcgtcca acgacagccc caaaaatcgg aattcatttt tccatataac
     1021 agcacttctg ttactgcggg atttcagagg gtaagaagca aattaggtat taaggatctg
     1081 cgataccatg atttgcgtag agaaggggca agtcgcttat ttgaggctgg ttttagtatt
     1141 gaggaagtcg cccaagttac agggcatcgt tcattaaacg tgctatggca ggtatatacc
     1201 gaactgtatc cgaaatcttt acataatcgt tttgaagagc tccaaaggag cagaaataag
     1261 accccttgac actgtttatc catacagcta aaaataacac tgtatacaaa cacagtatag
     1321 agggactttt atgcgtattg aaatctgcat agccaaagaa aaaatgacta aaatgccaac
     1381 cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac gttatgacga
     1441 tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac gcaccgcaga
     1501 taaggattct gcaaaaactt ttgttcagga gactctgaaa gatacctggg aatctgctga
     1561 cgagtggttt gttcactaat taacacgtaa aatcggtaac ggctggaaat cattcaatac
     1621 tcgcactatc gaaagttcgc cagccagccg cagcacgttc ttgcatacga cgtgcctgcg
     1681 gtttccgaga ccgttctaac cgtcatactt acacatcaac ttagcgccca tccagagaaa
     1741 ttatcttttt tgcattccga agattcaaag agttagccta attttccaga tagttttctc
     1801 gacaaatctg aggtattttg gttatcgata cagtgctttg gctgaggaac agcattaaat
     1861 tcaaccattt tattcatagg gattaaaatg caagatacag acgtttacgt atacataggc
     1921 gatgttcacc gctctggtca tcacgacatc accaaagcga ttaaagaaag aaaaactaac
     1981 catggtttac aaaaaaatgt tcttttttgc gtcgctacct atggcggtga tcccagcgca
     2041 ggatatagaa ttggccgcgc tttgcaacac aactatgagc acatcaccgt tctagttgtt
     2101 ggcccttgca aaagtgctgg aacattgatg gctgtcgccg ctcataagct cgtgattggt
     2161 gatatgggtg agcttggccc tctagatatc caacttaaga aaaatgatga gatcggcgaa
     2221 ttgagctcgg gccttgttat cgcaacggcg attgatgaaa taaaagagtg cgctatttcg
     2281 acctttagat catacgtact tgatataaag tacagaaacc aaattagcac aaagatgtct
     2341 gcagatattg ctgctaagct tactgaagca atcatatcgc cgatggcctc acagatagac
     2401 cctatcaaac tcggggagca tcagcgagca atgaacatag cgctttccta tggggaacgt
     2461 ttgaatagtc actcgaaaaa tcttaaggaa agctcattag gcaagttgat cgctggatat
     2521 ccagcccatg gttttgtgat agaccgtaaa gaggcgaggg aactattcaa ccatgttgag
     2581 agtcctacag attttgcagc caacctgtac gaagttcttg gtgaaatgat ttcgtcaggc
     2641 gaaatctcta tcttcgacaa accgatgatt aaagatttaa ctcctaaaaa acaatatgaa
     2701 acaactaacg cagaggaaga ttcccatgac aacagcagaa ttcaggaagg agatgaaatc
     2761 tctgaccaaa attcaggaag agcagaaccg ccaagggatg aacctgtcca gggaggcgat
     2821 ggaaaacatg cggaaactga agaagaggct ggaaccttac cagcagagca accagatgaa
     2881 ggcactcagt gaatattgta gtttctgatt gaggacctcg gctactacgc cggggtttct
     2941 ttttacatca ctggacagtc attaaactcc gtattcctaa catcattatg atgtacgtga
     3001 ttgctccgaa gattgagtgt gaataattgt agccaccacc atcatctggc agcgcttccg
     3061 ttcttcagat ttattaggtg ggaccaaggg tgagtacgat atcgcttaat cctgaaactc
     3121 aatttcagcc aaggccccca tcacataaaa gaaaaaacgc cccatcgacg tgctgatatc
     3181 aatagcatcc gttaagctgc gaaaattaac gccacgttcg cgcaactcct caaccagaat
     3241 gaccagatgc cgcatattac gccccagccg atccagcttc cagacaacca gagtgtcacc
     3301 ttccgataat gtcctgagca gttttttcag tcccggcctt tcagactttg taccgcttat
     3361 cttgtcttca aaaatcagct cacatcctgc acagttcagc gcattacgtt gtagatctgt
     3421 gttctggtca tttgttgaca cacgtacata gccaataagc atggtagatc tccctgacaa
     3481 aagcaggaat gatgccattt gctcgttatt tctgcatttt cataaac
//
LOCUS       DBBGLQ010000240         3512 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.240, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000240 DBBGLQ010000000
VERSION     DBBGLQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.240"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..652
                     /locus_tag="ACIYSB_005371"
     CDS             <1..652
                     /locus_tag="ACIYSB_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGC5432178.1"
                     /translation="QRFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLV
                     LWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPCHEWDMAGIQPSPAQAQ
                     NIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELC
                     TESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
     gene            1024..>3512
                     /locus_tag="ACIYSB_005372"
     CDS             1024..>3512
                     /locus_tag="ACIYSB_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HGC5432179.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTPVPALAADTVVEAGETVNGGTLTNHDNQIVFGTTNGMTISTGLEYGTDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTLVDDGGTLAVFAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNARGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTQGAVLSRAVAKGDAPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLESDLLRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAG"
BASE COUNT          801 a          905 c         1077 g          729 t
ORIGIN      
        1 gcagcgcttc cgtctgagtg gccatggtca cagcatggtt atcactgacc tgcccggggt
       61 gggcgagagc cgggacaggg atgcagagta tgaagccctg taccgtgaca ttctgcctga
      121 acttgacctg gtactgtggc tgattaaagc cgatgaccgt gccctgtctg tggatgagta
      181 tttctggcga cacatcctgc accggggaca tcagcgggta ctgtttgtgg tgacgcaggc
      241 cgataaaacg gagccctgcc atgaatggga tatggccggt attcagcctt ctcccgcaca
      301 ggcacagaac attcgcgaaa aaacggaggc ggtattccgt ctgttccggc ccgtacatcc
      361 ggttgtggcc gtatcggccc gcaccggctg ggaactggat acgctggtca gtgcgctcat
      421 gacagcactt cccgaccatg ccgccagtcc cctgatgacc cgcctgcagg acgagctgtg
      481 cacggaatct gtccgggggc aggcccgtga acagtttacc ggtgcggtgg accggatatt
      541 tgacacggcg gagagcgtct gtgttgcctc tgtggctcgt acggttctgc gtgccgtccg
      601 tgacacggtg gtctctgttg cccgcgctgt atggaactgg attttcttct gaacactctg
      661 ccaggggaaa tgcctgatta cattgcgtta accaactgaa aacatgacac aaacacctca
      721 tacccacctc tttctcttgc gtgactgcct tgctgttaat agaataaaac tatcgataaa
      781 acaggtatcg atcgttgtaa tcgatcgttt atggtggtta atggtctgcg aaaaggcagc
      841 tgaatctctt catcatgcag aacggaattg cacacaacgg actgatactt ctctggcgga
      901 tgagagaggg gaagcattta tgtccgggaa aaaccacttt atctgaccat gctgttttgt
      961 acctgccggt atccacattt gtgggtaccg gcttttttat tcaccctctg taaggaaaag
     1021 ctgatgaaac gacatctgaa caccagctac aggctggtat ggaatcacat tacgggcacc
     1081 ctggtggtgg cttccgaact ggcccgctca cggggaaaac gcgccggtgt ggcggttgcg
     1141 ctgtctcttg ctgctgtcac gccagtcccg gcactggctg ctgacacggt tgtagaggcg
     1201 ggagaaaccg tgaacggcgg aacactgaca aatcatgaca accagattgt cttcggtacg
     1261 accaacggaa tgaccatcag taccgggctg gagtatggga cggataacga ggccaatacc
     1321 ggcggacaat ggatacaaaa tggcggtatc gccaacaaca ctactgtcac cggtggtggt
     1381 cttcagagag tgaatgccgg aggaagcgtt tcagacacgg ttatcagtgc cggaggcgga
     1441 cagagccttc aggggcaggc agtgaacacc actctgaacg gcggtgagca gtgggtacat
     1501 gaaggcggga ttgcaacggg taccgtcatt aatgagaagg gctggcaggc cgtcaaatcc
     1561 ggcgcaatgg caaccgacac ggttgtgaat accggcgcgg aagggggacc ggatgcagaa
     1621 aatggtgata ccgggcagac cgtctacgga gatgccgtac gcaccaccat caataaaaat
     1681 ggtcgtcaga ttgtggctgc tgaaggaacg gcaaatacca ctgtggttta tgccggcggc
     1741 gaccagactg tacatggtca cgcactggat accacgctga atggggggta ccagtatgtg
     1801 cacaacggcg gtacggcgtc tggcactgtt gtaaacagtg acggctggca gattatcaag
     1861 gaaggtggtc tggcggattt caccaccgtt aaccagaaag gcaaactgca ggtgaacgcc
     1921 ggtggtacag ccacgaatgt caccctgaag cagggcggcg cactggtcac cagtacggcg
     1981 gcaaccgtca ccggcagcaa ccgtctgggg aatttcacgg tggaaaacgg taaggctgac
     2041 ggtgttgttc tggagtccgg cggtcgcctg gatgtactgg agggccattc agcgcagaaa
     2101 acactggtgg atgacggcgg taccctggca gtgtttgccg gtggtaaggc aacagatgtc
     2161 accatgacat ccggtggtgc cctgattgca gacagtggtg ccactgttga ggggaccaat
     2221 gccagcggta agttcagtat tgatggcata tccggtcagg ccagcggact gctgctggaa
     2281 aatggcggca gctttacggt taatgccaga ggactggcca gcaacaccac tgtcggacat
     2341 cgtggaacac tgacgctggc tgccggggga agtctgagtg gcagaacaca gctcagtaaa
     2401 ggtgccagca tggtactgaa tggcgatgtg gtcagtaccg gcgatattgt taacgcaggg
     2461 gagattcgct ttgataatca gacgacacag ggtgccgtcc tgagccgtgc tgttgcaaaa
     2521 ggcgacgccc cggtaacgtt ccataaactg accaccagta acctcaccgg tcagggtggc
     2581 accatcaata tgcgtgttcg ccttgatggc agcaatgcct ctgaccagct ggtgattaat
     2641 ggtggtcagg caaccggcaa aacctggctt gcgttcacaa atgtcggaaa cagtaacctc
     2701 ggggtggcaa cttccggaca gggtatccgg gttgtggatg cacagaatgg tgccaccaca
     2761 gaagaaggtg cgtttgccct gagtcgcccg cttcaggccg gtgcctttaa ctacaccctg
     2821 aaccgtgaca gcgatgaaga ctggtacctg cgcagtgaaa atgcttatcg tgctgaagtc
     2881 cccctgtatg cctccatgct gacacaggca atggactatg accggattct ggcaggctcc
     2941 cgcagccatc agaccggtgt aaacggtgaa aataacagcg tccgtctcag cattcagggc
     3001 ggtcatctcg gtcacgataa caacggcggt attgcccgtg gggccacgcc ggaaagcagc
     3061 ggcagctatg gcttcgtccg tctggagagt gacctgctga gaacagaggt tgccggtatg
     3121 tctgtgaccg cgggggtata tggtgctgct ggccattctt ccgttgatgt taaggatgat
     3181 gacggttccc gcgccggcac ggtccgggat gatgccggca gtctgggcgg atacctgaat
     3241 ctggtacaca cgtcctccgg cctgtgggct gacattgtgg cacagggaac ccgccacagc
     3301 atgaaagcgt catcggacaa taacgacttc cgcgcccggg gctggggctg gctgggctca
     3361 ctggaaaccg gtctgccctt cagtatcact gacaacctga tgctggaacc acaactgcag
     3421 tatacctggc agggactctc cctggatgac ggccaggata acgccggtta tgtgaagttc
     3481 gggcatggca gtgcacaaca tgtgcgtgcc gg
//
LOCUS       DBBGLQ010000241         3498 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.241, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000241 DBBGLQ010000000
VERSION     DBBGLQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.241"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            66..800
                     /locus_tag="ACIYSB_005373"
     CDS             66..800
                     /locus_tag="ACIYSB_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432180.1"
                     /translation="MKNYTADPFMRAIFGSGCFESGDKSTQDNNDNAMLESAGQKGKD
                     TQPKPKDDIETAMMEAVESRAQGDMRSLAASMIAGWVEDGDPEADSFDALAITMAGLA
                     DIDEDTDFTDEQIDAYNDALAALADAAVALGADQDDVTEMIDDEDDSAAERVYDALSE
                     SDTDMMETAIAIYTVAGGDSAMLEAVRKKVVRDGKVTIIRKRPRPRRMTSLQKQALKK
                     ARRKAHTSVANINRKKSMRIRKKRGL"
     gene            892..1440
                     /locus_tag="ACIYSB_005374"
     CDS             892..1440
                     /locus_tag="ACIYSB_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432181.1"
                     /translation="MKVYILSSEDMVVGYIGEGSTAELSSMWQSPFENQSVGGLLGGI
                     SAAAGSLADTLQTATGVTTKTLFNSMLVWEGQQPPEFNLVIDFMATTNAQLEVNAAIT
                     ALLKMESPELNNVAPFGRRPETVTLNIGRNIALTDVVIKSVSYQLDAPRTPEGYFTHN
                     TVTLQCSGNTSINRSNISSVFV"
     gene            1449..1940
                     /locus_tag="ACIYSB_005375"
     CDS             1449..1940
                     /locus_tag="ACIYSB_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGC5432182.1"
                     /translation="MSGFANTKADMAFLKSRFNKNLAAGEKLIGSEYWMTIKGYEHLS
                     VLVRTAQLPEMTREDVEDYAPGGMKFNQHGPLRNSGEFQVTCAETIEGAVLAAVKQMV
                     YGKEYLEITLQAAAESNSGNHKGLIRTYSHCKVYSDAVDFSSEDVTAVVKPSLRIVYN
                     WVE"
     gene            1993..2544
                     /locus_tag="ACIYSB_005376"
     CDS             1993..2544
                     /locus_tag="ACIYSB_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432183.1"
                     /translation="MTPTELLESVKARFNPLLVREEETLKAFLIKALTTYQDRAGVVK
                     TLKLEKAGGTAIPLPEDYLSLVHVTDNNGLLVYSDELSGFIELELTGSERWPFRMLYL
                     VNLRDRKLDEWQVPPAIIGMLEEYLEALINVRNVARQRRASIDGKFDYSDLPDETTLY
                     ARVQEIEEKMSSNRAIIPGATIF"
     gene            2560..3267
                     /locus_tag="ACIYSB_005377"
     CDS             2560..3267
                     /locus_tag="ACIYSB_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432184.1"
                     /translation="MSIFSSVGRTLTTALSFNTKSFTSNLISDILDKAISGGGVSGNY
                     SSDIAYGKNIVAAAMRIRYAQGWQWTVEVDGLNGFDMFVKDITYSSGNIETESKVIGS
                     VEFSKPTYVSAGPVTMTVRDTEDGKIMDWFKERRSRVTNPDGTINLPPEYLMKIRVYR
                     VTQDGGKELEEEMRVFPTQLGEITRSRDQVSEFLSYPITFQKYTSAGSGVSALVNGAA
                     GMATSALKGAVSGVIKF"
BASE COUNT          985 a          775 c          882 g          856 t
ORIGIN      
        1 taagccaatt ctgatgcgct gatttataac ccccgctaaa tgatgcgggg gagagaggtt
       61 tgaatatgaa aaattatact gctgatcctt ttatgcgtgc gattttcggt tctggctgct
      121 ttgaaagcgg tgataaatcc acgcaggaca ataatgataa cgccatgctg gaaagcgcag
      181 gacagaaagg aaaggacact caaccaaagc ccaaagacga cattgaaacc gcgatgatgg
      241 aagcggttga atcccgtgca caaggtgata tgcgcagcct ggcagcttcc atgattgctg
      301 gttgggttga agatggtgat ccggaggctg actcatttga tgcattagca atcactatgg
      361 ctggcctggc tgatatcgat gaagacaccg atttcaccga tgagcaaatc gatgcttata
      421 acgatgctct cgcagctctt gctgatgcag ctgttgcgct tggcgctgat caggacgatg
      481 taaccgaaat gatcgacgat gaagatgact cagctgccga acgtgtttat gacgctcttt
      541 ccgaaagcga caccgacatg atggaaacag ctattgccat ttacaccgtt gctggtggtg
      601 atagcgcaat gctggaagcg gtacgtaaaa aagtggttcg tgatggcaag gttaccatca
      661 tccgtaaacg cccgcgtcct cgccgcatga cgtcattgca aaaacaggcg ctgaaaaaag
      721 cacgccgcaa agcacataca tccgtagcga atatcaaccg caaaaaatca atgcgcattc
      781 gcaaaaaacg cggtttgtaa gtgacatagg tcgcctgcaa aggtggcctc tacctgggag
      841 aaatagcgtt tgatttgcgg cgcgataatg cctgacggaa taagtccgtt aatgaaggtt
      901 tatatcctct cttcagagga tatggtcgtc ggttatatcg gtgaaggatc taccgcagaa
      961 ctgtcatcaa tgtggcaatc accatttgaa aaccagtcag taggaggttt gcttggtggt
     1021 attagcgctg cggctggctc tcttgcagat acgcttcaga ccgcaaccgg cgtaacaaca
     1081 aaaaccctct tcaactcaat gcttgtttgg gaaggtcagc aaccgcctga atttaatctg
     1141 gtaattgatt tcatggcgac aacgaacgcg caacttgaag tgaacgcagc cattacagca
     1201 ttgctaaaga tggaatcgcc ggaactgaac aatgtagcgc cattcggtcg tcgcccggag
     1261 acagtaacgt taaacatagg taggaacata gcccttaccg acgttgttat caaaagcgtg
     1321 agttaccaac tggacgcccc gcgcacaccg gaaggctatt tcactcataa cacggtaacc
     1381 ctgcaatgca gcggtaacac atcgatcaac cgtagcaata tctcatctgt ttttgtgtag
     1441 gagtatttat gtccggtttt gcaaatacaa aagccgatat ggcctttcta aaatcccggt
     1501 ttaataaaaa cctggctgca ggcgaaaaac ttattggttc tgaatattgg atgaccatta
     1561 agggttacga acatctgtct gtactagttc gtacagctca gttaccagaa atgacacgcg
     1621 aagatgtgga ggattacgcc cctggtggca tgaaattcaa ccagcacgga ccattacgca
     1681 actctggtga atttcaggtc acatgcgcgg aaactatcga aggcgcagtt ctcgccgctg
     1741 taaaacagat ggtatacgga aaagaatatc tggaaattac cttacaggcg gcggctgaat
     1801 cgaatagcgg taaccataaa ggtctgatcc gcacatattc tcattgcaaa gtgtattccg
     1861 acgccgtgga cttctcatcc gaagacgtaa cagcggtcgt taagccttcc ctgcgtattg
     1921 tttacaactg ggtagagtga tctctttccc gcccttgtgg cgggaattcc ttgtatttga
     1981 gttgtaggta cgatgacacc aaccgaatta ttagaaagcg ttaaagcacg atttaatcca
     2041 ctgcttgtgc gtgaagaaga aacgctgaaa gccttcctga tcaaagctct gaccacgtat
     2101 caggacaggg caggggtagt gaaaacgtta aaacttgaaa aagctggtgg tactgcaatc
     2161 ccgttgccag aagattatct ctctctggtt catgttaccg acaacaatgg cttgttggtc
     2221 tattcggatg aattatccgg atttattgaa ttagagctaa caggttcaga acgctggcct
     2281 tttcgaatgc tgtatctggt taatctacgc gacagaaagc tggatgaatg gcaagtgcct
     2341 cctgcaatca ttggaatgct ggaggaatat ctggaggcgc ttatcaatgt aagaaatgtc
     2401 gctcggcagc gtagagcgtc catcgacggg aaatttgact actccgatct gcccgatgaa
     2461 accacgctat atgcccgcgt acaggagatt gaggaaaaaa tgtcctcaaa ccgggccatt
     2521 attccggggg ctaccatttt ttaacgctgg aggcgcagag tgagtatttt cagcagcgtt
     2581 ggtagaacgt tgaccaccgc attgtcattt aacacgaaat cattcaccag caacctcatt
     2641 agcgacattc ttgataaagc catatcaggc ggcggggtaa gcgggaatta cagtagcgat
     2701 attgcctacg ggaaaaatat tgttgctgcc gctatgcgta tccgttacgc ccaggggtgg
     2761 cagtggaccg ttgaagtgga tggcctgaac ggctttgata tgttcgtgaa ggatatcacc
     2821 tacagcagtg gaaacattga gacagaaagc aaggtgatcg gcagcgtaga attcagcaag
     2881 ccaacctatg ttagcgccgg accggtcact atgaccgtga gggataccga ggatggcaag
     2941 atcatggact ggttcaaaga gcgtcgttct cgtgtaacaa atcctgatgg aacaataaac
     3001 cttcctcctg aatatctgat gaaaatccgt gtctaccggg tgactcagga cggcggaaaa
     3061 gaactggaag aagaaatgcg cgtatttcct acgcaacttg gggaaatcac acgctcacgt
     3121 gatcaggtgt cagagttctt gtcataccca atcactttcc agaaatacac atccgcaggc
     3181 tcaggtgttt cagctttggt caacggtgcc gctggcatgg ctacaagtgc gttaaagggg
     3241 gctgtgagtg gagtgatcaa attctgaata gctttaatta tgtgtgctgg agggttgctc
     3301 atttcccgtc agcacccacg ccctcaacga tagcgggtag ggaaggtagc gcaattttga
     3361 gggcattttt tacatcatta atccatctgt aatgaaggca ttctttacac atttcaataa
     3421 tatcaatcag ttaacacagt gaatgataat aaaaagcgca ataagaatgc acgatacgca
     3481 cgatattggt tgagggcg
//
LOCUS       DBBGLQ010000242         3485 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.242, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000242 DBBGLQ010000000
VERSION     DBBGLQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.242"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            38..1156
                     /locus_tag="ACIYSB_005378"
     CDS             38..1156
                     /locus_tag="ACIYSB_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HGC5432185.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLSAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPASQEMQETSATPAITADKNTLVAEARRNLAIVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNRTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            1137..3410
                     /gene="trbC"
                     /locus_tag="ACIYSB_005379"
     CDS             1137..3410
                     /gene="trbC"
                     /locus_tag="ACIYSB_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HGC5432186.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHIIFFSTTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFITQQSMILARDLGYRLEGTDAQTLEVKKYKGRFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQKQLNETQTSLDLLPQYDTDISARGNS
                     LWNKAVHIINTTSREERHVCYITQSCPEEERVEEKMPPSVAQTMERIFTSNLQNEATA
                     KEEISHHVTEQQEWPGH"
BASE COUNT          895 a         1001 c          872 g          717 t
ORIGIN      
        1 ccgaaaaaca gcatcccgcc gacaaggaaa aagcacaatg accatccgct acactgcaac
       61 actgaacaat aaacacctgt ccctgaccgc cctgtaccct gacggtggtc aggaggttat
      121 tgccatcctg ccggttacag aactgggcgc actgattaac cgtaataaca ggctgatgca
      181 cctgtccttc agcacccctt ttcgtgaaat aaccgaaaca ctgcttgttg ataccgttga
      241 tactgaacgt cccggacatc acatcggcag aatgctctcc gcgattgaca gctgtctgag
      301 cgcggcatat gcagaacttc ctgcccggaa aaccagcttc atgagagggc tgatatacgg
      361 tgcaggatta gtgctggcac ttctgacagc ggtaacagcc ggatatattt tactgaaacc
      421 ggacagcgat tcccctctca gggtacctgt cacagaatcc gtaacagaaa cagcaccatt
      481 acacatcgac aaggaaattg tccggtcagt agccccgtca gagacgaaaa atgaaatcgt
      541 tcacccggca tcacaggaaa tgcaggagac atccgctacc ccggcgataa cagcagacaa
      601 aaatactctg gtcgctgaag cccgccgtaa tctggccatt gtacttaaac gcaacgcgga
      661 ccggggtatg ttcaccatta atctctccac tggtcatgaa cgcacactct acgccttcct
      721 tgaccccgca tgcatcaact gccgtcttct ggagcccgcg cttaagcaac tggcccggga
      781 attcagcgtg gtgatttatc cggtctccgt aatcggcgga gaggccagca caaaccggac
      841 tgcgccactg ctgtgtgaac cggacgcaca aaaacgggcg accggatggc atcagctgta
      901 ttccgcagac ggtggtatgg cacccaccgg cgatgcaccg gctgcaccgg atgaaaagtg
      961 tctgaccgct gctcgtgctg ccatcgatgt caataaccag gctttccgcc agttcggttt
     1021 tggcggtacc ccatgggtgt tatccgatac cggctggcac ctgccgtccg gcatcctgaa
     1081 agacgccact accgtcagcc tgttcctgaa agccacagat accgaggata atcacaatga
     1141 gccagcatca cgttaatcct gacctcatac accgcacggc ctggggaaac ccgctgtgga
     1201 atgcactgca caacctgaac atcattggtc tgtgtctggc cggcagtatc attacggccc
     1261 tgatatggcc actggccctt cctgtctgtc tgctgttcac cctggtcacc tccgtgatat
     1321 tcacgctgca acgctggcga tgccccctgc gtatgcccat gacgctcagc cttgatgacc
     1381 cctcccagga cagaaaggtc aggcgaagcc tcttcagctt ctggcccacc ctgtttcagt
     1441 atgaagcaga tgaaacatcc cccgcacggg ggatttttta tgtcggttac cgccgtatca
     1501 acgatatcgg tcgtgagctc tggctgagca tggatgacct cacccggcat atcatatttt
     1561 tttccaccac cggtggcggt aaaacagaaa ccacctttgc ctggctgctc aaccctctct
     1621 gctggggaag aggctttacc tttgttgacg gaaaggcaca gaacgatacc acgaggacaa
     1681 tctggtatct ctcccgacgt tttggccggg aggatgatat cgaggtcatc aacttcatga
     1741 acggcggaaa atcccgcagt gaaatcattc agagcggcga aaaatcccgc ccacagtcaa
     1801 acacctggaa cccgttcgca ttcagcaccg aagccttcac cgccgaaacc atgcagtcca
     1861 tgcttcccca gaacgttcag gggggtgaat ggcagtcacg ggcgatagcc atgaacaaag
     1921 ccctggtctt tggcacaaaa ttctggtgcg tccgggaaag aaaaaccatg tccctgcaga
     1981 tgttacggga gcatatgacg ctggaaggta tggcaaaact ctattgccgt gggcttgatg
     2041 accagtggcc ggaagaagcc atcgcaccac tgagaaatta cctgcaggac gttccgggat
     2101 tcgatatgtc actggtgaga acgccgtcag cctggactga ggagccccgt aaacagcatg
     2161 cttatctctc cggtcagttc tcggaaacat tcacaacctt tgccgaaacc ttcggggacg
     2221 tctttgcggc agatgccggc gacatcgata ttcgcgacag tatccacagc gaccgaattc
     2281 tgattgtgat gatccccgca ctggacacat cagcccatac cacgtcggca ctcggtcgta
     2341 tgttcatcac ccagcagagc atgatcctcg cccgtgacct gggctaccgc ctggaaggca
     2401 cagacgcaca gaccctggaa gtaaaaaaat acaaaggccg cttcccctat atctgtatcc
     2461 ttgacgaagt cggtgcctac tacacagacc gcattgctgt ggaggccacc caggtacgtt
     2521 cactggagtt ttccctgata atgatggcgc aggatcagga gcgaattgaa gggcagacct
     2581 ccgccaccaa caccgccaca ctgatgcaga acgccggcac caaattcgcc ggacgcattg
     2641 tcagtgacga caaaacggca agaacggtga aaaatgccgc cggtgaagag gctcgggcca
     2701 gaatgggcag cctgcagcgc cacgatggtg tgatgggtga gtcctgggtg gacggtaacc
     2761 agatcaccat ccagatggaa agcaaaatcg acgtgcagga tttgatcaga ctgaatgccg
     2821 gtgagttctt taccgttttc cagggggatg tggtgccgtc agcctctgtg tatattccgg
     2881 acagcgagaa aagttgcgac agtgatccgg tggtcatcaa ccgctatatt tccgttgaag
     2941 ccccccggct ggagcgtctc cgccgtctcg tccccaggac agtgcagcgc aggctcccca
     3001 caccggagca tgtcagcagc attattggtg tactgacagc aaaaccatcc cgtaaacgtc
     3061 gtaaaaaccg tacagagcct taccgtatca tcgatacctt ccagaagcag cttaacgaaa
     3121 cacaaacatc gctggatctg cttccgcaat atgatacaga tatctctgcc cggggaaaca
     3181 gcctgtggaa caaggcagtt cacatcatca ataccacatc ccgggaagaa cggcacgtat
     3241 gctatatcac acagagttgt ccggaagagg aacgtgtgga agaaaaaatg ccaccatcgg
     3301 ttgcacaaac aatggagcgt atatttactt caaacctcca gaatgaggct acagctaaag
     3361 aagaaatatc acatcacgtc actgaacaac aggagtggcc cggacattaa tattgacatt
     3421 cctccacatt cgtcagggcg tggaggatgt caaagacctg aattgattgt gtgcttagct
     3481 tcact
//
LOCUS       DBBGLQ010000243         3485 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.243, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000243 DBBGLQ010000000
VERSION     DBBGLQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.243"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(16..915)
                     /gene="cynR"
                     /locus_tag="ACIYSB_005380"
     CDS             complement(16..915)
                     /gene="cynR"
                     /locus_tag="ACIYSB_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGC5432187.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPSITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            1024..1683
                     /gene="cynT"
                     /locus_tag="ACIYSB_005381"
     CDS             1024..1683
                     /gene="cynT"
                     /locus_tag="ACIYSB_005381"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGC5432188.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            1714..2184
                     /gene="cynS"
                     /locus_tag="ACIYSB_005382"
     CDS             1714..2184
                     /gene="cynS"
                     /locus_tag="ACIYSB_005382"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGC5432189.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDSILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            2190..3371
                     /gene="cynX"
                     /locus_tag="ACIYSB_005383"
     CDS             2190..3371
                     /gene="cynX"
                     /locus_tag="ACIYSB_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGC5432190.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPMQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPATAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
BASE COUNT          705 a          946 c          980 g          854 t
ORIGIN      
        1 gacctgtgtc agcggctacc gtgattcatt tccgccaaca accgcgcatt tatccaacgc
       61 catgtgcaaa aatgccttcg cggcggctgt ctgccagcta tttttccgcc gcaacaaaac
      121 cgccgttctc tccagtagtg gcggggcaag agaaatagct ttaagcccgt catgttgtgt
      181 ggcaatcgct gctggtaaca atgtggaaag ggaagtgcgg cgaatcagct ccagaaccgc
      241 gctaattgag ttcgcctcaa tgaccacctg tggatgtagc cccgctttct cgcagtagtg
      301 gtcaatttgc tctctggtgg caaattccgc gctgagcagg accagttttt catcatgcaa
      361 gcgactcaac gccacctgtt catggacggc cagcggatga tgttgcgcca cgactaacgc
      421 taaactttct gtcagtaaag gaattgcctc cagctccggc gaatgcacag gcgcgaaggc
      481 aatcccaacg tccaactcgt cgcggcaaag catatcctcg attttctcct gcgacatttc
      541 ctgtagctgg agcgtgatgc tgggatagcg cgcatagaaa tccgccatta aggggccgat
      601 aaagtagctc gtaaaggtgg gggtgacggc gatacgcagc gatcctcgcg tcagatcggc
      661 aacatcatga atcgcccgtt tacccgcccc cagttcctgt aacgcccggc tggcgtactg
      721 tcgccagact tctcctgcat cagtgagacg aatcgttctc ccgctacggt caaacagcgg
      781 cacgcctaaa ctctcctcta actggcgaat ctgctgggaa agcgcaggtt gggagacgtg
      841 caacgcactg gcggcacggg tgaagctgcc atgttcagcc acggcaagaa aataattgat
      901 atgtcgagag agcattcgca acctataagt aaatccaatg gaactcatca taaatgagac
      961 ttttacctta tgacaatcgg cgagtagtct gcctctcatt ccagagacag acagaggtta
     1021 acggtgaaag agattattga tggattcctt aaattccagc gcgaggcatt tccgaagcgg
     1081 gaagccttgt ttaaacagct ggcgacacag caaagcccgc gcacactttt tatctcctgc
     1141 tccgacagcc gtctggtccc tgagctggtg acgcaacgtg agcctggcga tctgttcgtt
     1201 attcgcaacg cgggcaatat cgtcccttcc tacgggccgg aacccggtgg cgtttctgct
     1261 tcggtggagt atgccgtcgc tgcgcttcgg gtatctgaca ttgtgatttg tggtcattcc
     1321 aactgtggcg cgatgaccgc cattgccagc tgtcagtgca tggaccatat gcctgccgtc
     1381 tcccactggc tgcgttatgc cgattcagcc cgcgtcgtta atgaggcgcg cccgcattcc
     1441 gatttaccgt caaaagctgc ggcgatggta cgtgaaaacg tcattgctca gttggctaat
     1501 ttgcaaactc atccatcggt gcgcctggcg ctcgaagagg ggcggatcgc cctgcacggc
     1561 tgggtctacg acattgaaag cggcagcatc gcagcttttg acggcgcaac ccgccagttt
     1621 gtgccactgg ccgctaatcc tcgcgtttgt gccataccgc tacgccaacc gaccgcagcg
     1681 taaccttatt tttaaaccat caggagttcc accatgattc agtcacaaat taaccgcaat
     1741 attcgtcttg atcttgccga tgccattttg ctcagcaaag ctaaaaaaga tctctcattt
     1801 gccgagattg ccgacggcac cggtctggca gaagcctttg taaccgcggc tttgctgggt
     1861 cagcaggcgc ttcctgccga cgccgcccgc ctggtcgggg cgaagctgga tctcgacgaa
     1921 gactccattc tactgttgca gatgattcca ctgcgtggct gcattgatga ccgtattcca
     1981 actgacccaa cgatgtatcg tttctatgaa atgttgcagg tgtacggtac aaccctgaaa
     2041 gcgttggttc atgagaaatt tggcgatggc attattagcg cgattaactt caaactcgac
     2101 gttaagaaag tggcggaccc ggaaggtggc gaacgtgcgg tcatcacctt agatggtaaa
     2161 tatctgccga ccaaaccgtt ctgacagcca tgcgcaacca tcaaaagacg ttcacgatgc
     2221 tgctggtact ggtgctgatt ggtcttaata tgcgaccact gctcacctcc gtcgggccac
     2281 tgctaccgca attgcgccag gcgagcggaa tgagctttag cgtggctgcc ctgttgaccg
     2341 ctctgccggt ggttaccatg ggcgggctgg cgctggccgg aagctggctt catcagcatg
     2401 tcagcgaacg tcgcagtgtc gccatcagtc tgttgctgat tgccgtcggt gcattgatgc
     2461 gtgagcttta cccgcaaagt gcgctgctgc ttagcagcgc actgcttggt ggggtgggga
     2521 tcggcatcat tcaggcggtg atgccttcgg tgattaaacg gcggtttcag cagcgcacgc
     2581 cactggtgat ggggctgtgg tccgcggctc tgatgggcgg cggtgggctt ggtgccgcca
     2641 taacgccctg gttagttcaa catagcgaaa cctggtatca aacactcgcc tggtgggcgc
     2701 tgcctgccgt tgttgcgctc tttgcctggt ggtggcaaag cgcccgcgag gtcgcctctt
     2761 cccacaagac aacaaccact ccggttcgcg tggtattcac tccccgcgcg tggacgctgg
     2821 gtgtttactt cggtctgatt aacggcggtt acgccagcct gattgcctgg ttacccgctt
     2881 tctatattga gattggtgcc agcgcgcagt acagcggttc cttactggca ttgatgacgc
     2941 ttgggcaagc cgcaggagct ttgctgatgc ctgctatggc tcgccatcag gatcggcgca
     3001 aactgttaat gctggcgctg gtgttacaac tggtggggtt ctgcggcttt atctggctgc
     3061 cgatgcaatt gccggtattg tgggcgatgg tgtgtgggtt aggtctgggc ggcgcgtttc
     3121 cgctctgttt gctgctggcg ctcgatcact ctgtgcaacc ggctactgct ggcaagctgg
     3181 tggcgtttat gcagggaatc ggttttatca tcgccgggct tgccccgtgg ttttctggcg
     3241 tgctgcgtag tatcagcggc aattacctga tggactgggc atttcatgcg ctgtgcgtcg
     3301 ttgggctgat gatcataacc ctgcgttttg caccagtacg ttttccgcag ctgtgggtca
     3361 aagaggcatg atgcgacgct tgttcctgcg ctttgttcat gccggatgcg gctaatgtag
     3421 atcgctgaac ttgtaggcct gataagcgca gcgtatcagg caatttttat aatttaaact
     3481 gacga
//
LOCUS       DBBGLQ010000244         3421 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.244, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000244 DBBGLQ010000000
VERSION     DBBGLQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.244"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            138..1103
                     /gene="dusB"
                     /locus_tag="ACIYSB_005384"
     CDS             138..1103
                     /gene="dusB"
                     /locus_tag="ACIYSB_005384"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGC5432191.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1129..1425
                     /gene="fis"
                     /locus_tag="ACIYSB_005385"
     CDS             1129..1425
                     /gene="fis"
                     /locus_tag="ACIYSB_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGC5432192.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACIYSB_005386"
     CDS             1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACIYSB_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGC5432193.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            2479..2658
                     /locus_tag="ACIYSB_005387"
     CDS             2479..2658
                     /locus_tag="ACIYSB_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGC5432194.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="ACIYSB_005388"
     CDS             complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="ACIYSB_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HGC5432195.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          938 a          789 c          809 g          885 t
ORIGIN      
        1 attatcgcac cacgctcatt ttaaatgcaa ttctttgatc catctcagag gattggtcaa
       61 agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc agtcacagta
      121 tggtcatttc ttaactcatg cgcatcggac aatatcagct cagaaatcgc ctgatcgcag
      181 cgcccatggc tggcattaca gacagacctt ttcggacgtt gtgctacgag atgggagccg
      241 gattgacagt atccgagatg atgtcttcta acccacaggt ttgggaaagc gacaaatctc
      301 gtttacggat ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg
      361 atccgaaaga aatggcagat gcagcacgta ttaacgtgga aagcggtgcc cagattattg
      421 atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt tcagccctct
      481 tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc
      541 ctgttaccct gaagattcgc accggctggg caccggaaca ccgtaactgc gaagagattg
      601 cccaactggc tgaagactgt ggcattcagg ctctgaccat tcatggccgt acacgcgcct
      661 gtttgttcaa tggagaagct gagtacgaca gtattcgggc agttaagcag aaagtttcca
      721 ttccggttat cgcgaatggc gacattactg acccgcttaa agccagagct gtgctcgact
      781 atacaggggc ggatgccctg atgataggcc gcgcagctca gggaagaccc tggatctttc
      841 gggaaatcca gcattatctg gacactgggg agttgctgcc cccgctgcct ttggcagagg
      901 ttaagcgctt gctttgcgcg cacgttcggg aactgcatga cttttatggt ccggcaaaag
      961 ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct ccaaatgacc
     1021 agtttcggcg cacattcaac gccattgagg atgccagcga acagctggag gcgttggagg
     1081 catacttcga aaattttgcg taaacagaaa taaagagctg acagaactat gttcgaacaa
     1141 cgcgtaaatt ctgacgtact gaccgtttct accgttaact ctcaggatca ggtaacccaa
     1201 aaacccctgc gtgactcggt taaacaggca ctgaagaact attttgctca actgaatggt
     1261 caggatgtga atgacctcta tgagctggta ctggctgaag tagaacagcc cctgttggac
     1321 atggtgatgc aatacacccg tggtaaccag acccgtgctg cgctgatgat gggcatcaac
     1381 cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga actaattcag gttagctaaa
     1441 tgcttgatta aaaaggcgct actcggcatg gggaagcgcc ttttttatag gtgtcacaaa
     1501 gggagtgacc atgagaacag gatgtgaacc gacccggttt ggtaatgaag ctaagaccat
     1561 tattcacggt gatgcgcttg ccgaacttaa aaagatcccc gccgaaagtg tcgatctgat
     1621 ctttgccgac ccaccatata acatcggtaa aaattttgat ggtctgatcg aagcctggaa
     1681 agaagatctg tttatcgact ggctgtttga agtgattgca gagtgccacc gcgttctgaa
     1741 aaagcagggc agcatgtaca tcatgaacag tacggaaaac atgcccttta tcgatctcca
     1801 gtgccgcaag ctttttacca tcaaaagtcg catcgtctgg tcatatgaca gttctggagt
     1861 gcaggcgaaa aaacactacg gctccatgta cgaacccatc ctgatgatgg tgaaagacgc
     1921 aaagaactac acattcaacg gtgatgctat tctggtagaa gccaaaaccg gatcgcagcg
     1981 cgcgttgatc gattatcgca aaaatcctcc acagccttac aatcatcaaa aagtaccggg
     2041 taacgtttgg gattttccgc gcgtgcgtta tttgatggat gaatatgaaa accacccgac
     2101 gcaaaaaccg gaagccttac tgaaacgcat tattctcgcc tcttccaacc caggcgatat
     2161 cgttctcgac ccgtttgctg gtagctttac taccggtgcc gtagccatcg ccagcggacg
     2221 aaaattcatt ggtattgaga tcaacagcga gtacatcaaa atggggcttc gacggctgga
     2281 tgtcgcgtcg cattactctg cggaagaact ggcgaaagtg aaaaaaagaa agaccggcaa
     2341 cctgtcaaaa cgaagccggt taagcgaagt tgaccccgat ctcattacaa agtaaaggat
     2401 gtgtaagcct ggttttcaga ttattcattt cgtgtatatt tcctgccaga cttggttaaa
     2461 catgcacagg ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt cgctgtcttc
     2521 gtttttctgt ttgatacttt actgatgcag tggattgaac tgctggcaac agaaacagac
     2581 aaatgccgca atatgaactc agttaatcca ctaaaactgg ttaactgtga cgaactgaat
     2641 tttcaggaca gaatgtgaat ttacatgaca cttaattcat tcgtttgatg aattaatttc
     2701 gttatgtttt catctggcat gaacattctt aatacgttat cgaccagagc gggggcttgt
     2761 ttataaagat cataacccgc catattcatt aaccagtttt gaacaattcc gctgaaggca
     2821 ccatcaataa taatcatcac aacatctaaa tcgaggttat ttgctacaca accttgttgc
     2881 tgacacgcct gcaatacttc gcggagagtc tgcggattaa agcccatctt ttcgcgtatc
     2941 actccctcgg ccagcatctc atcattaaat tcacatttgt gatataagat tttcagcaac
     3001 gcctgctggc ggggaatttt ggcaatatat tgcaagccga caatcaattt ttcacgcaat
     3061 tgttgaaacg ggtcatgctc taatccagcc gtcaagtgtt cctggattaa ctcccgcaat
     3121 gaaggctgtt gcaaccacat ctcattaaac agttgagtct tgttttcgaa gtgccagtag
     3181 atagcgccac gcgtaacgtt agcggcgtcg gcaatgtcgt tgagcgtcgt cttgcttacg
     3241 ccatgctgcg caaactgggc gatggcagtt tcaatcagtt cttgccgggt cttcagagct
     3301 tcggctttgg ttctttttgc catgattaat tattcaggaa ataaatatat tcgacacaga
     3361 gtgagaaaat agcgaaggtt aatctatcac ctaatgtgta tttatacgag aggctaatat
     3421 t
//
LOCUS       DBBGLQ010000245         3420 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.245, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000245 DBBGLQ010000000
VERSION     DBBGLQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.245"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..555)
                     /locus_tag="ACIYSB_005389"
     CDS             complement(<1..555)
                     /locus_tag="ACIYSB_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGC5432196.1"
                     /translation="MKIALMNEFSQASKNAIIFNELVAASAHQGHTIFNVGMCDDNDH
                     RLTYVHLGIISAILLNSRSVDFVITGCGTGQGALMALNTFPGVTCGYCIEPTDAYLFA
                     QVNNGNALSLPFAKGFGWGAELNMRYIFEKAFDGEKGLGYPAERRESQNANAIILSNM
                     KEAVSKPLMDALQAIDPELLKQALG"
     gene            828..1982
                     /locus_tag="ACIYSB_005390"
     CDS             828..1982
                     /locus_tag="ACIYSB_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005968909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HGC5432197.1"
                     /translation="MNLSTIAFYDSESNNEVIRLTPTDILCHRNYFYQKCFTNDGKKL
                     IFAVEFDCNRNYYLMDLVTQTAKQFTEGAGDNTFGGFLSPDDNYLFYVKNEKHLQRVD
                     LTTLEEVTIYTVPDNWVGYGTWVANTDCTEIVGIEISKTDWTPLSDWKIFLEFYHKNP
                     RCRLITIDLETGNAEVILDRNTWLGHPIFRPNNNDTIAFCHEGPHDLVDARMWMINRD
                     GTNERKVHEHQPGESCTHEFWIPDGSALAYVSYMKGQQQRYIRKYNPDTDEDSLVMEM
                     PACSHLMSNHNGILYVGDGSGSPVDVKDTCSYKIENDPWIYILSSKNKKIHKLVKHNS
                     SWRVQKGDRQVTHPHPSFSPDNKYVLYSCDAEGEPALYMAKIPEEILTQQ"
     gene            2012..2812
                     /locus_tag="ACIYSB_005391"
     CDS             2012..2812
                     /locus_tag="ACIYSB_005391"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGC5432198.1"
                     /translation="MDFINMFSLKGKNALVTGASYGIGFEIAKALSNAGATIIFNDVI
                     EENIKKGLAAYKENNINAHGYLFDITDEQSVKENIKKIESDVGIIDILVNNAGIIQRK
                     PMLETTAADYRKIIDIDLTGQFIMAKAVLPSMIKKGHGKIINICSMMSELGRETVVGY
                     ASAKGGLKMLTKNICSEFGEKNIQCNGIGPGYIATPQTAPLREIQPDGSRHPFDQFII
                     SKTPAGRWGKPDDLAGAAIFLASDASNFINGHILYVDGGILAYIGKQP"
     gene            2867..3190
                     /locus_tag="ACIYSB_005392"
     CDS             2867..3190
                     /locus_tag="ACIYSB_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGC5432199.1"
                     /translation="MFVFNEDTAIEDLGNGVKRKVLAHGGNMMAVEVHFIKGAIGSLH
                     HHQHEQLTYVLSGIFEFTIGDEVRTVKTGDTLYKQPNIVHGCKCLEKGVLLDIFTPQR
                     QDFLK"
BASE COUNT         1096 a          593 c          681 g         1050 t
ORIGIN      
        1 accaagagct tgtttaagta actccggatc gatggcctgg agagcatcca tgagtggctt
       61 actaactgct tctttcatat tactcaggat aatggcattc gcattttgtg attcacgacg
      121 ttctgccgga tagcctaatc ccttttcgcc atcaaatgct ttttcaaaaa tatagcgcat
      181 gtttaattct gccccccagc caaaaccctt tgcaaatggc aaggacagtg cattcccatt
      241 atttacctgt gcaaacaggt aggcatctgt tggttcgatg caataaccac aagtaactcc
      301 cgggaatgtg ttcagtgcca tcaatgcccc ctgtcctgta ccacagccgg taataacgaa
      361 atcaacagat ctgctgttta acaagatagc cgaaataatg ccgagatgaa cgtaagtcag
      421 acgatgatca ttgtcatcac acattcctac attgaaaata gtatggcctt gatgtgcgct
      481 tgctgcaact aactcattaa aaattattgc gtttttagat gcctggctaa attcattcat
      541 caatgcgatt ttcatacgaa ttcctcattt aaaatcatac tgttaaactt catagcataa
      601 caacatgaac gatatgataa tacattacaa acattacttg caacattgta tttgaaaatt
      661 caaaacatca tttcattttt attgttgtgt gctctcgatc acggtttttt gcacttttga
      721 acgctatatt ggatgctaac gaaacgatag aaacgatatg atgaagtatt gtcattctta
      781 taaaggataa gattatatgg ctaagggtga tattaaaaag ttgaagtttg aatctttcta
      841 cgatagcttt ctacgatagt gaatcgaata acgaagttat tcgattaacg ccaacagata
      901 ttctttgcca tcgcaattat ttttatcaaa aatgttttac aaatgatggt aaaaaattga
      961 ttttcgctgt tgagtttgat tgtaaccgga attattatct tatggatttg gtaactcaga
     1021 cagcgaaaca attcactgaa ggagctggag ataacacctt tggtggcttc ctgtcccctg
     1081 atgataatta tcttttttat gtgaagaatg aaaaacattt gcagcgtgtt gatttgacca
     1141 cattagaaga agttaccata tacacagtac cggataattg ggtggggtac ggaacatggg
     1201 ttgctaatac tgattgtacg gaaatcgtcg gaatcgaaat cagtaaaact gactggaccc
     1261 cgcttagcga ctggaaaata tttctggaat tttatcataa gaatccacga tgccgtttaa
     1321 ttacgattga tctggaaacc ggaaacgctg aagtgatatt ggatcgtaat acttggttag
     1381 ggcatccaat ttttcgtccg aataataacg atactatcgc cttttgccac gaagggcccc
     1441 atgacctggt ggatgcaaga atgtggatga ttaaccgtga tgggacaaat gagcgtaaag
     1501 tgcatgagca ccaaccaggt gaaagttgca cacacgagtt ctggatccct gatggatctg
     1561 cactggctta tgtttcctat atgaaaggac agcaacagcg ttatattcgc aaatataatc
     1621 ctgatactga tgaagattca ttagtgatgg aaatgccggc atgttcccat ttaatgagta
     1681 accataatgg tattctctat gttggtgatg gttcaggtag tccggttgat gttaaagata
     1741 cctgcagtta taaaattgaa aatgatccct ggatttacat tcttagcagt aaaaataaaa
     1801 aaatacataa attggtgaaa cataatagtt cctggagggt acagaaagga gaccggcagg
     1861 taacgcaccc gcatccatca ttttctccag ataataagta cgttttatat tcttgcgatg
     1921 ccgaaggtga accagcacta tatatggcta aaattcctga ggaaatctta acccagcaat
     1981 aactgacaaa tttttcacta ttggagtttt tatggatttc atcaatatgt tctctcttaa
     2041 aggtaaaaat gctttagtca ctggagcatc atatggtatt ggttttgaga tcgctaaagc
     2101 actaagtaac gctggtgcaa cgatcatttt taatgatgta atagaagaaa acattaaaaa
     2161 aggcctggca gcatataaag agaacaacat caacgcacat ggttatttat ttgatattac
     2221 agatgaacag tctgtgaagg aaaatatcaa aaaaatagag agtgacgtag gcattattga
     2281 tattctggta aataatgcag ggattattca gcgtaaacct atgctagaaa ctacagcagc
     2341 tgattatcgc aaaataatag atattgatct aacaggtcag tttattatgg ctaaggcggt
     2401 attgccttca atgattaaaa aaggacatgg caagatcatt aatatctgtt ccatgatgag
     2461 cgaattgggt cgcgaaaccg ttgtaggcta cgcttcggca aaaggcggct taaaaatgct
     2521 gactaaaaat atctgttctg agtttggtga aaaaaatatt cagtgtaatg gtattggtcc
     2581 aggctacatt gctacacctc aaacagcacc gttacgtgaa attcaaccag atggcagccg
     2641 ccatccattt gatcagttta ttatatcaaa aactccagct ggacgctggg gtaaaccaga
     2701 tgatcttgct ggtgcagcta tatttttagc atcggatgct tcaaacttta tcaatggtca
     2761 tattttatat gtcgatggtg gcattctggc ctacattggc aaacagcctt agttatattc
     2821 agtcataact gtatgtacta ctttaaatat tttcagagag gtggtgatgt ttgtatttaa
     2881 tgaagataca gcaatagaag atttaggcaa cggggtcaaa agaaaagttt tggctcatgg
     2941 tggcaatatg atggcagtag aggtccattt cattaagggg gctatcggct ctttacatca
     3001 ccaccaacat gaacagttga cgtatgtctt atcaggtatt tttgaattta ccatcggcga
     3061 tgaagtgcgt acagtaaaaa ctggtgatac tttatataag cagcccaaca ttgtgcacgg
     3121 gtgcaaatgt ctagaaaaag gagttttgct tgatatattt acacctcaac gtcaggattt
     3181 tttaaagtaa atacctggta ttaatatgaa ctaaaatggt agcgcataaa atgcgctaca
     3241 tattgatata gttaactaat tatctattgt ttttctttct gccatagaca gctttaatct
     3301 gaaaaatatt atcttttaaa tggaaatttt agaaataata gaatctgatt atccggatat
     3361 ttagttgaat tctataaagt cgagtgaaca tattgttgta tcaatatagt taatttaaat
//
LOCUS       DBBGLQ010000246         3372 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.246, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000246 DBBGLQ010000000
VERSION     DBBGLQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.246"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..181
                     /locus_tag="ACIYSB_005393"
     CDS             <1..181
                     /locus_tag="ACIYSB_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGC5432200.1"
                     /translation="APDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLW
                     SDADPVPPWIWRHRK"
     gene            227..436
                     /gene="hha"
                     /locus_tag="ACIYSB_005394"
     CDS             227..436
                     /gene="hha"
                     /locus_tag="ACIYSB_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGC5432201.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            475..977
                     /locus_tag="ACIYSB_005395"
                     /pseudo
     CDS             475..977
                     /locus_tag="ACIYSB_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
     gene            1258..1407
                     /locus_tag="ACIYSB_005396"
     CDS             1258..1407
                     /locus_tag="ACIYSB_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGC5432202.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            1691..1939
                     /locus_tag="ACIYSB_005397"
     CDS             1691..1939
                     /locus_tag="ACIYSB_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGC5432203.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQEMCDAEGVTQAEMIAELIKQKSAFS"
     gene            2185..2259
                     /gene="tap"
                     /locus_tag="ACIYSB_005398"
     CDS             2185..2259
                     /gene="tap"
                     /locus_tag="ACIYSB_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGC5432204.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCG"
     gene            2252..3109
                     /gene="repA"
                     /locus_tag="ACIYSB_005399"
     CDS             2252..3109
                     /gene="repA"
                     /locus_tag="ACIYSB_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGC5432205.1"
                     /translation="MADLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKALGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALMFLSELGLITYQTEYDPLIGCNIPTDITLTPSLFTALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDALSMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDASRERQEIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          885 a          815 c          886 g          786 t
ORIGIN      
        1 tgcaccagac ggaatgaata ttaatcagtt tatggtccgg gccggggctg cctgggtata
       61 tgagcagtac aacacagatc ccgtgctgcc tgtccttcag aacgaagcca gacaacaaaa
      121 acgtgggctc tggtcagatg ctgatccggt tcccccctgg atatggaggc accgtaaata
      181 aagcgatggc aatcctatca ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa
      241 acaggaatgg ctgtatcagt tacggcgatg ttcatcagtc aatacgctgg aaaaaattat
      301 tcataaaaac agagacagtt tatcaaacag tgaacgtgag tcatttaatt cagcagcaga
      361 ccaccggtta gcggaattaa tcaccggaaa gttatatgac aggataccaa aagagatatg
      421 gaaatatgta aggtaataat tcagcaataa ttaattgcca ggagcagata aataatgaag
      481 ttgattattt ttattctcat tgttctgatt attgcagcgt tacttatccg gattatcctc
      541 agaagtgtaa atcagcattc tcctttgctg atgcaattgc atacggcagg tatacggacc
      601 ggtgatgcag agcgaatact gtccagcggt ggtatctgtg tccgcaggta cgggtcgcgg
      661 atatcgtcca gctgaacggg aatatccggc cacgatcacg ccagtggtgg cagttattca
      721 ggatggtgtc tcagtggcat gttgatgtgg ttatcgttga gcggcgttcg ttcagtattg
      781 ttgcagcagt agagctggat gatgccagcc atttacgacc ggaacgcaga cgccgggata
      841 ttcttctgga agaggttctg aggcaggctg gtattccgtt gctcagaagc cacgatgcca
      901 gaaaactgct gcagatgacc ggagaatggc tgaatacaac aggggctgat cagcagtcac
      961 cggaacatcg tagctgacgc cttcgcgttg ctcagttgtc caaccccgga aacgggaaaa
     1021 agcaagtttt ccccgctccc ggcgtttctg caataaaaac cgggggcatg gagatcgtcg
     1081 taaccagaca ggaatgtggt attatcgcgg cgggtgtctg agcctttctg gttcaggcaa
     1141 gacgcaggta ccagaaatgc gaagacccca ctcgttaatc cattaactcg tgaggtctgc
     1201 atgaagtacc ttaacactac tgattgtagc ctcttctttg cagagaggtc aaagtttatg
     1261 acgaaatatg cccttatcgg gctgctcgcc gtgtgcgcca cggtgttgtg tttttcactg
     1321 atattcagag aacggttatg tgagctgaat attcacaggg gaaatacagt ggtgcaggta
     1381 actctggcct acgaagcacg gaagtaagct gccgggcggg gacggaagtc cccgctttcc
     1441 ggaagtgtga ggtatttcag gggcagacac ccgacatgcc agaaacagcc ggtcccgccc
     1501 ggggccggca tccatgttaa ggcatttcct gtttttcagt catttcatca tcaaaatcac
     1561 attaaacggt cgtaatcaga catgatttgt gcgccaacac agatcatcat cacaactttc
     1621 aagtcgctga tttcaaaaaa ctgtagtatc ctctgcgaaa cgatccctgt ttgagaattg
     1681 aggaggcgag atgtcgcaga cagaaaatgc agtgacttcc tcatcaggta caaagcgagc
     1741 ataccgaaaa ggtaatcctc tgacgcttgc agaacgtcag caggcatctc tggcaagaaa
     1801 acgtgccaca cataaagaac tcagagtgtt cattcctgct gcgttaaaag cgcaattaca
     1861 ggagatgtgt gacgctgaag gtgttactca ggctgagatg attgctgaac tgataaagca
     1921 gaagagtgct ttcagctaac taaaatgtag cgacatcaca ttcttgcctg tttctggtgt
     1981 gcgtgataga ttgctgatcg tttaaggaat tttgtggctg gccacgccat aaggtggcag
     2041 ggaactggtt ctgatgtgga tgtacaggag ccagaaaagc gaaaaccccg ataatcttct
     2101 tcaactttgg cgagtacgaa aagattaccg gggccccata aaaccgcata gccaacaatt
     2161 cagctatgcg gggagtatag ctatatgccc ggaaaagttc aagacttctt tctgtgctca
     2221 ctccttctgc gcattgtaag tgcaggatgg tgtggctgat cttcaacaaa cgtattaccg
     2281 ccaggtaaag aacccgaatc cggtgtttac accccgtgaa ggtgccggaa cgctgaagtt
     2341 ctgcgaaaaa ctgatggaaa aggcgctggg cttcacttcc cgttttgatt tcgccattca
     2401 tgtggcgcat gcccgttcgc gtggtctgcg tcgacgcatg ccaccggtgc tgcgtcgacg
     2461 ggctattgat gcgctgctgc aggggctgtg tttccactat gacccgctgg ccaaccgcgt
     2521 ccagtgctcc atcaccacac tggccattga gtgcggactg gcgacggagt ctgctgccgg
     2581 aaaactctcc atcacccgtg ccacccgtgc cctgatgttt ctgtcagagc tggggttgat
     2641 tacctaccag acggaatatg acccacttat tggctgcaac attccgaccg acatcacact
     2701 cacaccgtcg ctgtttaccg ccctcgatgt gtcagaggat gcggtggccg ccgcgcgtcg
     2761 cagtcgtgtg gaatgggaaa accgacagcg taaaaagcag gggttggatg cgctgagtat
     2821 ggatgagctg atagcgaagg cctggcgttt tgtgcgtgag cgtttccgca gttaccagac
     2881 agagcttaag tcccggggaa taaagcgtgc ccgtgcgcgt cgtgatgcaa gcagggaacg
     2941 tcaggaaatt gtcaccctgg tgaaacggca gctgacacgt gaaatctcag aagggcgctt
     3001 cactgccaat cgtgaggcgg taaaacgcga agtggagcgt cgtgtgaagg agcgcatgat
     3061 tctgtcacgt aaccgtaatt acagccggct ggccacagct tccccctgaa agtgacctcc
     3121 tcagaataat ctggcacgcg ccggaggcat tcgcacgcct gaagcccgtc agtgcacaaa
     3181 aaatcagcac cacatacaaa aaacaacctc accatccacc ttctggagca tctggccccc
     3241 cctgttttcg atacaaaaca cgcctcacag acggggaatt ttgcttatcc acattaaact
     3301 gcaagggact tccccataag gttacaaccg ttcatgtcat aaagcgccag ccgccagtct
     3361 tacagggtgc aa
//
LOCUS       DBBGLQ010000247         3366 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.247, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000247 DBBGLQ010000000
VERSION     DBBGLQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.247"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(18..563)
                     /locus_tag="ACIYSB_005400"
     CDS             complement(18..563)
                     /locus_tag="ACIYSB_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGC5432206.1"
                     /translation="MKLPVKRYAVAAIVALGVSMAPGELRTSREAQIKIATREECRAT
                     PYRDIAGVMTVGCGSTGGVENRVYGEKEVARRWVNDLRHAENCINQNFSGAAMPQSAF
                     EAMTDAAFNVGCTGLMWYRDRSGNRQRTTIWKHAQAHRWVAMCGRLTDFVNSGGRRSQ
                     GLVNRREEFRQWCLRDTGGST"
     gene            complement(560..850)
                     /locus_tag="ACIYSB_005401"
     CDS             complement(560..850)
                     /locus_tag="ACIYSB_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432207.1"
                     /translation="MVTGFFQRVSDWITFWASVTATTIGVMTISEKIALAGLLLGSLS
                     AIHAWCHRRRTEQAMKRRNDLIARILARSENRELHDGERRALDSVRQDGEER"
     gene            complement(844..1041)
                     /locus_tag="ACIYSB_005402"
     CDS             complement(844..1041)
                     /locus_tag="ACIYSB_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGC5432208.1"
                     /translation="MKVRALQGDTVDLLCFRHYGTTQGVTAQVLDANPGLCRQVILEA
                     GQEVEMPEPERKQQREKVKLW"
     gene            complement(1041..1535)
                     /locus_tag="ACIYSB_005403"
     CDS             complement(1041..1535)
                     /locus_tag="ACIYSB_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGC5432209.1"
                     /translation="MSMVARPEAAPARDDITDTDDGDATITAGAFWPEIVLRELRLAV
                     RLPGRITSTRLAHVATGAVAHVTRELEEWQQAQIAAGFSTLTDVPAATINGESVNAWH
                     YRHAVYSATRALILERWRDVDTTDKGDRRADALDEQVEDLWRDVRWAISDILGFPRLF
                     VELV"
     gene            complement(1639..2421)
                     /gene="gpM"
                     /locus_tag="ACIYSB_005404"
     CDS             complement(1639..2421)
                     /gene="gpM"
                     /locus_tag="ACIYSB_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HGC5432210.1"
                     /translation="MLTPAQAHFQHVMALRAGTAAENEDIVARTAHEQVLHRLRLAQQ
                     RIKGVQAKAAKAALKREMLPGFAGWIDGTLEADGGQQDEVIATLMVWAVDCGDLALAL
                     RIGDYVVRHNLTMPDNFGRTAATVLTEEICNPVLTQAGTDAHADLSGYIEPLDTLWQI
                     VANRDMPDEVRAKLCKACAFARRGLTDADSMAVSLKLFREAMHLNPNAGVKREIATLS
                     RALKKAGQDEAAAAQEPAAQKDTTREQTTTPKAKAKEKAKRG"
     gene            complement(2424..>3366)
                     /locus_tag="ACIYSB_005405"
     CDS             complement(2424..>3366)
                     /locus_tag="ACIYSB_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HGC5432211.1"
                     /translation="QKGEKVLLDTTGPVARTNSSYDGTNRRNPLNVVDMKARRYECEQ
                     VNYDTFISYPQLDMWAGHPDFQLRVSRQIARQVALDRIMTGFNGTHHAEVSDIKKHPR
                     LEDVNKGWLQDIRDQAPQRVMKDVMLTARNMDNSVAYTGRYANPDALVQDARSSLLDE
                     WHKDADDLVVIMGRNLFNSLRLPVLNSISMQNANAELLAGQLIVSTRTIGGLDVYLAP
                     FFPAQSMLITSFSNLSVYWQKGSMRRLMKDEPEYNRIAIYQSVNDCYVVEDHGKCALI
                     ENISFATYDHDAPDWYREDIKALSAQAEDKDAGEEED"
BASE COUNT          596 a         1056 c          945 g          769 t
ORIGIN      
        1 cacggcgtaa accggtctca tgtgctgcct cccgtgtcgc gaagacacca ctgtctgaac
       61 tcctcccggc ggttaaccag cccctgtgag cggcgtccgc cactgttgac aaaatcggtc
      121 agtctcccgc acatcgccac ccagcggtgc gcctgtgcgt gcttccagat ggtggtgcgc
      181 tgccggttgc cgctcctgtc ccggtaccac atcaggccgg tacagccgac gttgaacgcc
      241 gcgtcggtca tcgcctcaaa ggcggactgg ggcatggcgg caccgctgaa gttctggttg
      301 atacagtttt ccgcgtgccg caggtcattc acccagcggc gggccacctc cttttctccg
      361 taaacccggt tctccacgcc ccctgttgaa ccacatccca cggtcattac tcctgcaata
      421 tcacggtacg gtgtcgcccg gcactcttcc cgggtggcga tttttatctg tgcttcccgt
      481 gaggttctca gttcaccggg tgccatggag acgcccagcg ccacaatggc cgccaccgca
      541 taacgtttta ccggcagttt caccgctctt ctccgtcctg tctcacgctg tccagtgccc
      601 gtctctcacc gtcgtgaagc tcgcggtttt ctgaacgtgc caggatgcgg gcgataaggt
      661 cattgcggcg cttcattgcc tgctccgtac gcctgcggtg gcaccaggca tggatggcgg
      721 aaagtgaccc cagaagcagc ccggccagcg caattttttc gctgatggtc ataacaccaa
      781 tggttgttgc cgtcactgac gcccagaagg tgatccagtc actgacgcgc tgaaaaaatc
      841 ccgtcaccat agctttacct tttcgcgttg ctgtttcctt tccggctccg gcatctccac
      901 ctcctgtccc gcttccagaa ttacctggcg gcagagcccg ggattggcat caagtacctg
      961 tgcggtcacc ccctgcgtgg tgccgtagtg ccggaaacag agtaaatcga cggtgtcccc
     1021 ctgaagtgcc ctcactttca tcacacaagc tccacaaaaa gacggggaaa gccgagaata
     1081 tcggaaatcg cccagcgcac gtcgcgccac aggtcctcca cctgctcatc cagtgcgtca
     1141 gccctgcggt cccccttgtc ggtggtgtcc acatcccgcc agcgctccag aatcagggca
     1201 cgggtggcgc tgtagacggc gtgccggtaa tgccaggcgt tgacgctctc accgttgatg
     1261 gtggcggcag gtacgtctgt cagcgtgctg aatcctgcgg cgatttgcgc ctgctgccac
     1321 tcttccagct cccgcgtgac gtgagccacg gctcccgtgg caacgtgcgc cagacgtgtg
     1381 gaggtgatgc ggcccggcag gcgtactgca aggcgcagct cccgcagcac aatctccggc
     1441 cagaatgccc cggcggtgat ggtggcgtcg ccgtcgtcgg tgtcggtgat gtcgtcccgc
     1501 gcaggggcgg cttccggtct tgcgaccatg ctcataaatc gctccttcag aaaaaaaagg
     1561 cggtgggtgc cgggtgtaag gggaacggaa aaccgtaacc ggaacgcccg gcacgccgcc
     1621 ttctgacggg gtcagccgtt aaccacgttt cgccttctct ttcgctttcg ccttcggcgt
     1681 cgtggtctgt tctctggttg tatctttctg tgccgcaggc tcctgtgcgg cagcggcttc
     1741 atcctgtccg gcctttttca gggcccgtga aagcgtggca atctcacgtt ttacccccgc
     1801 attcgggtta aggtgcattg cctcacggaa cagcttcaga ctcaccgcca tgctgtcggc
     1861 atcggtcagc ccacgacggg caaaggcgca tgccttgcac agcttggcgc ggacctcatc
     1921 gggcatgtcc cggttcgcga caatttgcca gagggtgtcc agcggttcga tgtagccgga
     1981 caaatcggcg tgtgcatccg ttccggcctg tgtcagaaca gggttgcaga tttcctcggt
     2041 cagtaccgtg gctgccgtac gcccgaagtt gtcaggcatc gtgaggttgt ggcgaaccac
     2101 ataatccccg atgcgcagtg caagcgccag gtcgccacag tccacggccc acaccatgag
     2161 tgtggctatc acctcatcct gctgtccgcc gtcggcttcc agcgtgccgt ctatccagcc
     2221 tgcaaaaccc ggcagcattt cacgcttgag cgcggccttt gccgcttttg cctggacgcc
     2281 ctttatgcgt tgctgtgcca ggcgcagacg gtgaaggacc tgctcatgag ccgtgcgggc
     2341 cacgatatcc tcgttttctg ctgccgttcc tgcccgtaaa gccatcacgt gctggaaatg
     2401 agcctgtgcc ggtgtcagca tggttaatcc tcctcttccc ctgcatcctt gtcttcagcc
     2461 tgcgctgaca gtgcttttat gtcctcccgg taccagtccg gcgcgtcatg gtcatatgtg
     2521 gcaaagctga tattctcaat cagtgcgcac ttaccatggt cctcaaccac atagcagtcg
     2581 ttaacggact gatagatggc gatacggttg tattcgggct catccttcat gagacgacgc
     2641 atggacccct tctgccagta aaccgacagg ttgctgaatg aggtgatgag cattgactga
     2701 gccgggaaaa atggcgcaag gtacacatca aggccgccaa tggtccgcgt tgacacaatg
     2761 agctgtccgg caagcagttc ggcattcgca ttctgcatgc tgatactgtt caggaccggc
     2821 aggcgcaggg agttaaagag gtttcgcccc atgatgacaa cgaggtcgtc tgcgtccttg
     2881 tgccattcgt caagcaggga tgagcgtgca tcctggacca gtgcatcggg attggcgtat
     2941 ctgcctgtgt atgccaccga attgtccata ttgcgcgcag tcagcatcac gtctttcatg
     3001 acgcgttgcg gggcctggtc cctgatatcc tgcagccacc ccttattgac gtcctcaagt
     3061 cgcgggtgtt tttttatatc cgagacttcc gcatggtgcg taccgttaaa acctgtcatg
     3121 atgcgatcaa gtgccacctg tctggcaatc tggcgactca cacgcagctg gaagtcaggg
     3181 tgtccggccc acatgtcaag ctgagggtag gagatgaagg tgtcgtagtt cacctgctcg
     3241 cactcatagc gtcgcgcttt catgtccacc acattcagcg ggttacgacg atttgtaccg
     3301 tcataactgc tgttcgtgcg cgcaaccggc ccggtggtgt ccaggagtac cttttcacct
     3361 ttctgc
//
LOCUS       DBBGLQ010000248         3340 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.248, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000248 DBBGLQ010000000
VERSION     DBBGLQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.248"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..608
                     /locus_tag="ACIYSB_005406"
     CDS             <1..608
                     /locus_tag="ACIYSB_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGC5432212.1"
                     /translation="LILVTPVNGLPSSGVADTLTGGFDTEELETWRARVIERYYWTPQ
                     GGADGDYVVWAKEVPGITRAWTYRHWMGTGTVGVMIASSDLINPIPEESTETAARQHI
                     GPLAPVAGSDLYVFRPVAHTVDFHIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKV
                     SRISEAISGANGEYSHQLLAPADNISIAKNELAVLGTISWT"
     gene            599..1183
                     /locus_tag="ACIYSB_005407"
     CDS             599..1183
                     /locus_tag="ACIYSB_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HGC5432213.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPSIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINENFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            1187..1987
                     /locus_tag="ACIYSB_005408"
     CDS             1187..1987
                     /locus_tag="ACIYSB_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432214.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKARHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDD
                     GRGMDTGRAILSAQGDAIRNIYGEFKTVNTENYSIWESVGSFKGAVVPLNPSTNNSYF
                     SLIRSMVTERTDGAVYPKVIGLDASRIVPTANENRPRNIAFNYIVRAA"
     gene            1987..2589
                     /locus_tag="ACIYSB_005409"
     CDS             1987..2589
                     /locus_tag="ACIYSB_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGC5432215.1"
                     /translation="MNKAVLNNELIAIKAGVITIYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPVTHKAGYAICRSADFNSWEYVPDHRGEIIYSTETGESKEITAPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQPENTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(2561..3001)
                     /locus_tag="ACIYSB_005410"
     CDS             complement(2561..3001)
                     /locus_tag="ACIYSB_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGC5432216.1"
                     /translation="MQKYIFSADKNAFFPVELKIAYQESGEWPGDGIEIDDTVAAEFM
                     KEAPEGKYRGVIDGMPAWIDIPPPTHEEQIAAAELEKQQLINQVNEYINSKQWPGKAA
                     IGRLKGEELAQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(3004..>3340)
                     /locus_tag="ACIYSB_005411"
     CDS             complement(3004..>3340)
                     /locus_tag="ACIYSB_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432217.1"
                     /translation="RNVGTGDNQIPDMGAFASGSGWFRLPGGYIVQFGTFSGNTTRFI
                     SGHFPIPFPNQPMVSVSVMSDAVQSDPSNPAPQVLSVNFEHISNSAWRVATSDISQQY
                     RFSYISIGR"
BASE COUNT          831 a          841 c          879 g          789 t
ORIGIN      
        1 cattaatcct ggtcacgccg gtgaatggtc tgccgtcttc cggtgtggct gacaccctga
       61 caggcggatt tgatactgaa gagctggaaa cgtggcgcgc ccgcgtcatt gagcggtatt
      121 actggacgcc gcagggcggg gctgacgggg actatgtcgt ctgggctaaa gaagtgcccg
      181 gcattacccg cgcatggaca taccgtcact ggatgggaac gggaactgtc ggtgtgatga
      241 ttgccagcag tgacctgatt aatcccattc cggaagaatc aacggaaacg gcggcaagac
      301 aacatatcgg gccactggcc ccggtggcag gctctgattt gtatgtgttc aggccggtgg
      361 cacatacggt ggattttcat atccgcgtga cgccggacac accggaaata cgggctgcca
      421 tcaccgcgga gttgcgttcg ttcctgctgc gtgatggtta tccgcaggga gaactgaagg
      481 tatcgcgtat cagtgaggcg atttccggtg cgaacgggga atacagccat cagttgcttg
      541 caccggcaga caatatctcc attgcaaaaa atgaactggc ggtactgggg acgatttcat
      601 ggacgtgaca aacgatgatt acatccgtct gttgtcggca ctgttgcccc ccggtccggc
      661 gtggtcagcc agcgatccgt cgattgccgg tgcggcaccg tcattaaccc gcgttcatca
      721 gcgtgcggat gccctgatgc gggagctgga tccgcgcacc accactgaac tgataaaccg
      781 ctgggagcgt ctgtgcggtc tgccggatga atgtattccc gcagggacac agacccttcg
      841 ccagcgtcag caacggctgg atgcgaaggt taacctggcg ggcggcatca acgagaattt
      901 ttatcttgca cagcttgctg ccctgggcag accagacgct accatcacgc gatacgacaa
      961 aagcacgttc acctgctcat cggcctgtac tgacgcggtg aatgcgccgg aatggcggta
     1021 ttactggcag gtcaacatgc cagccgccac aaacaccacc tggatgacat gtggcgatcc
     1081 ctgtgattcc gcactgcgta tctggggcga caccgttgtc gaatgtgtgc ttaacaaact
     1141 ctgtccgtct catacctacg taatttttaa atatccggag taatccatgc atcgcataga
     1201 cacgaaaacc gcgcagaagg ataagttcgg cgcgggtaag aacggtttta cccgtggcaa
     1261 cccccagacc ggcacgcctg ccaccgatct ggatgatgac tactttgaca tgttgcagga
     1321 agagctttgt agcgtggtgg aggcatccgg tgccagcctg gagaaggcgc ggcacgacca
     1381 gctgcttacc gcgcttcgtg cgctgctgtt aagccgcaag aatccgtttg gcgatatcaa
     1441 atcggatggc acggtgaaaa cggctctcga aaaccttggt ttgggagaag gctctgcatt
     1501 acctgttggg gttcctgttc cgtggccttc agccactccg ccaacaggct ggctgaaatg
     1561 caacggagca gctttttctg ctgaagaata cccggagctg gcaaaggctt acccgacaaa
     1621 taaattgcct gatttacgtg gtgagtttat tcgtggctgg gatgacggac gtggtatgga
     1681 tacggggagg gcaatattat ccgctcaggg cgatgccata cgtaatatct atggtgagtt
     1741 caagactgta aacaccgaaa attattcaat atgggaatca gtaggctcgt ttaagggggc
     1801 agtagtgcct ttgaacccct caacgaacaa tagttatttc tccttaatca gaagtatggt
     1861 gacagaaaga acagatggcg ctgtttaccc aaaagtgatt ggccttgatg cttcaagaat
     1921 tgttccaact gcaaacgaaa accgtcctcg taacattgcc tttaattata tcgtgagggc
     1981 tgcctgatga ataaagccgt attaaataac gaactcattg ccataaaagc gggagtcatt
     2041 accatttata attatgatgg tgaaacgcgg gaatatattt ccacatcaac tgaatatctt
     2101 gcggttggcg tcggtatccc ggcatgttct tgtttagatg caccagttac acataaagct
     2161 ggttatgcaa tctgccgttc tgcagatttt aactcatggg aatatgtgcc agaccatcgc
     2221 ggtgaaatca tctatagcac cgaaacagga gaatcgaaag aaatcacagc tccgggtgat
     2281 tatcctgaaa atacaaccac tatcgccccg ttaacgccat acgataaatg ggatggtgag
     2341 aaatgggtga cggataccga ggcacagcat agcgccgcag tagaagcggc agaagcacag
     2401 cgccagtcgc tgattgatgc tgcaatggct tccatcagtc tgattcagct gaaattacag
     2461 gccgggcgga agctgacgca gccagaaaac acccgactta acgctgtgct ggattatatt
     2521 gacgcggtga cggcaacaga taccagcaca gcgccggacg tcatctggcc tgaactgccg
     2581 gaggcgtagg ccattcaata tctggcgcac cggaagtatc gaccagttcc agtgcgtcca
     2641 gataatccag ccacaaatta tattgcgcca gttcctcacc tttcaggcga ccaatcgccg
     2701 ctttaccagg ccattgctta ctgtttatgt attcgttgac ctgattaatc aattgctgct
     2761 tttccagttc ggctgcggca atttgttcct catgagttgg cggtggaata tcaatccatg
     2821 caggcattcc gtcgatgaca cctctgtatt ttccttctgg tgcttccttc ataaattcgg
     2881 cggcaacagt gtcgtcaatt tcgattccat caccgggcca ttcgccggat tcctgataag
     2941 cgattttaag ctccacaggg aaaaacgcat ttttatcggc actgaaaata tatttctgca
     3001 tttctaccgt cctatcgaaa tataactgaa tctgtattgc tgtgagatat cactggttgc
     3061 cacacgccac gctgaattac tgatatgttc aaaatttaca gacaaaacct gcggggcagg
     3121 attcgacggg tctgactgaa cggcatcaga cataacactg actgaaacca tcggctgatt
     3181 agggaatggt atagggaagt gtccactgat aaagcgggtc gtgtttcctg aaaaagtgcc
     3241 aaactgaaca atatatccac ctggtagcct gaaccatccc gaaccagaag cgaatgctcc
     3301 catatccggt atctgattat ctcctgtgcc cacattccgt
//
LOCUS       DBBGLQ010000249         3268 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.249, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000249 DBBGLQ010000000
VERSION     DBBGLQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.249"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..816
                     /locus_tag="ACIYSB_005412"
                     /pseudo
     CDS             1..816
                     /locus_tag="ACIYSB_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1952..2518
                     /locus_tag="ACIYSB_005413"
     CDS             1952..2518
                     /locus_tag="ACIYSB_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGC5432218.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENMVGFFWVIEWTVDHGFHAHVVFWLDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            2776..>3268
                     /locus_tag="ACIYSB_005414"
     CDS             2776..>3268
                     /locus_tag="ACIYSB_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432219.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKIIGVLNRVLREQSCSRIRRILLR
                     LPGEHGNIFSDRKTWFGMAFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSCSPDCEERLKELIWAF
                     SDFA"
BASE COUNT          830 a          761 c          737 g          940 t
ORIGIN      
        1 tcaacgtatt accactgtca gcaacagcga catcatcctg ataaacgcag tgcacatgca
       61 cagcgcgatg actggctgaa gagagagata cagcgcgtat acgatgaaaa tcatcagtgt
      121 accgtgtgcg taaagtctgg cgccagttgt tacgggaagg aatcaggatg gccagatgta
      181 cagtggtgcg cctcatggcg gttatgggac ttgccggtgt tctccggggt aaaaaggtcc
      241 gcactaccgt cagccggaaa accgttgccg caggtgaccg cgtaaaccgt cagttcgtgg
      301 cagaacgtcc tgaccagctg tgggtggctg attttactta cgtcagcaca tggcagggct
      361 tcgtctatag tggcgttcat cattgatgta tttgccggat atatcgtggg gtggtgggtc
      421 tcatcgtcta tggaaacgac attcgtgctg gatgcgctgg agcaggcgtt gtgggcccgc
      481 cgtccgtccg gcaccatcca tcacagcgat aaaggctctc agtatgtgtc actggcctat
      541 atggagcgat taaaagaagc aaaactgctg gcatcaacag ggagtacagg cgactcgtat
      601 gacaacgcga tggcggagag catcaatggt ctttacaaag cggaggtaat acaccgtaag
      661 agctggaaaa accgtgcaga agtggaactg gccacactca cgtgggtgga ctggtataac
      721 aatcgacgat tgctgggaag gctgggccat actcctccgg cagaagcaga aaaagcttat
      781 tatgcttcca tcggaaacaa tgatctggca gcctgagttc acagataaaa cactctccag
      841 gaaacccggg gcggttcagg actggagtgc cttaagtgct ttatcgaaat cgaagggttg
      901 ggacatgtgt cattcctttt tggttgtata ttactggaat gacacagaat ttctaacact
      961 ccctttaatt taggtttatt actattatta tacttactct cttttttaca tgacctcaat
     1021 ctttacgatt atattcactc tatctttaca cattccatta ctcttatcct catccaatta
     1081 tattcatgtc cttctctaat cttccttgtc accattatca taatatccac ttcttccctc
     1141 attcaatcat aattactgta attcatacgt tactcagata agttattctc tacagctacg
     1201 ctattgttga tatccatctg ttctcatttt cagtgaaaca gtaattgatt ttaatttatc
     1261 catcatgaac tgtatttgct taacaatgat tgtttatctg aagtgtttta actattctgg
     1321 ttggaaacaa tttctctgtc atcaaagatt aactgaatgt ttactctttg ataaggtatc
     1381 catggttccg tcatgtttaa cagcgcagga taaacaacag aattaacaga gtgaatttct
     1441 gattatattt gttgccggtt gtattgttta aggtactggg tgaaaattat tcatccattg
     1501 tatgttgtct tatgctatcg tgtgtcgtta acgttcatat cctggagaac agattgaata
     1561 agcgcgtgtg agtttattgt ggtgacctta taaacgtttt ttcagactca ctgagagcaa
     1621 atttacaaat catgagctta tagttgtaat atgtttccta accgcctggc agcgggatta
     1681 cagttcgtat ttttctgtat ttcactgtgt tgtattgaat ctgtattttc ttcgcacctc
     1741 gtttatctca tctctataga caatgccatc agataactca tacccttcag cacaacgctg
     1801 tgtgtcgtta ataatccgtc gtatccattc tgttacagca tgcctgataa ataccatctg
     1861 taagttatta ccgttttaga tctgattatg agcgaaagca ttaattcgct cacagagctt
     1921 aaaacatcat taactttcag gagtcatcaa catgcctaaa tcttacacac caaactggtt
     1981 ttttaccgct ttacttgaca atcacatcaa tcaaatgatg gcacgctatt cctgcctgcg
     2041 ggccttacgg atggatttct tctacaggaa ggatacgcct gatttcttac aacctgatca
     2101 tcgttggctt gaattacaac tgcgtatgct gctggagcag gttgaacagt ttgaaaatat
     2161 ggttggcttc ttctgggtga ttgaatggac ggttgatcat ggttttcatg cgcatgtggt
     2221 cttctggctc gatcgtcaga gggttaaaaa aatatatccc tttgcggagc ggattacgga
     2281 atgctggcgg tctattacgc ataacagcgg ttcggcacac cgctgtacat atcagccgca
     2341 ttatacatac aacatcaaca ttcctgtgcg ccacaacgat cctgaaagca tcgataatat
     2401 tcgcggtgcc ctgcattatc tggcaaaaga agagcaaaaa gacgggctgt gtgcctacgg
     2461 ctgcaatgaa gttcctgaac gtcctgctgc agggcgtcct cgtaagcctc acttctgaag
     2521 cttaaatcct gagcttcctg tcctggaaac attccgtcgg taaaacctta ccgccttgct
     2581 taatgatgtg aactgaagtc aacggagatc attcactctg aaccacatcc ggtgttctgt
     2641 cccttgtcgg tgacctgatc ttcccgttct gccccggttc ttcacttatt ccatcaaccc
     2701 catccgcaaa ccataacacg tcagcttatt cacgggcagg acgcattgtg ggctgcgcat
     2761 aacggagcat atcttatgaa tgctatccct tatttcgact atagcctggc ccccttctgg
     2821 ccatcttatc agaacaaaat catcggcgtt ctcaaccgtg tgttgcgcga gcagtcctgc
     2881 tcccgaatac ggcggatcct gcttcgtctg ccgggggaac atggcaacat cttcagcgac
     2941 agaaaaacct ggttcggtat ggcctttatc gaaaccgtca gtgcgctgat gaatgcaaca
     3001 cccggacgtg acctctgctg gctcctgaca cgtcatcctg aaaaaccgga ataccacgtg
     3061 gtgctgtgcg tcagacagga gtatttcgac ggccctgaac tggatcggct gattctggat
     3121 gcctggagca acgtactggg cttcgcatca ccgggtgaag cagcaccgta ccagaagcag
     3181 atcacccggg atgtggtgct ggacagttgt tcaccggact gcgaagagag actcaaagag
     3241 ctgatatggg cgttcagtga tttcgccc
//
LOCUS       DBBGLQ010000250         3234 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.250, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000250 DBBGLQ010000000
VERSION     DBBGLQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.250"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..942
                     /locus_tag="ACIYSB_005415"
     CDS             <1..942
                     /locus_tag="ACIYSB_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGC5432220.1"
                     /translation="SVPNDELFGIIYTVDEGDDWTNPQVLEKANPNIGVSVYREFLLS
                     QQQRAKNNARLANVFKTKHLNIWVSARSAYFNLVSWQSCEDKSLTLEQFEGQPCILAF
                     DMARKLDMNSMARLYTREIDGKTHYYSVAPRFWVPYDTVYSVEKNEDRRTAERFQKWV
                     EMGVLTVTDGAEVDYRYILEEAKAANKISPVSESPIDPFGATGLSHDLADEDLNPVTI
                     VQNFANMSDPMKELEAAIESGRFHHDGNPIMTWCIGNVVGKNMPGNDDLVKPVKEQAE
                     NKIDGAVALIMAVGRAMLYEKEDTLSDHIESYGIRSL"
     gene            954..1136
                     /gene="ymfR"
                     /locus_tag="ACIYSB_005416"
     CDS             954..1136
                     /gene="ymfR"
                     /locus_tag="ACIYSB_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HGC5432221.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            1136..2377
                     /locus_tag="ACIYSB_005417"
     CDS             1136..2377
                     /locus_tag="ACIYSB_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGC5432222.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGIYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            2355..3005
                     /locus_tag="ACIYSB_005418"
     CDS             2355..3005
                     /locus_tag="ACIYSB_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGC5432223.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            3020..>3234
                     /locus_tag="ACIYSB_005419"
     CDS             3020..>3234
                     /locus_tag="ACIYSB_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGC5432224.1"
                     /translation="MAVDIKDVEQIAQELQQKFDDFKVKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPA"
BASE COUNT          788 a          792 c          956 g          698 t
ORIGIN      
        1 tcggtaccca acgatgaact gttcgggatc atctataccg ttgacgaagg tgacgactgg
       61 accaacccgc aggtgctgga aaaagccaat ccaaatattg gcgtgtcggt ttatcgcgaa
      121 tttttgttaa gtcagcagca gcgtgcgaaa aataacgccc gtctggcaaa cgtctttaaa
      181 acaaaacacc tcaatatctg ggtgtcggcg cgttcggcgt atttcaacct ggtgagctgg
      241 cagagctgcg aggataaatc actgactctt gagcagttcg aggggcagcc gtgcattctg
      301 gcctttgaca tggcgcgtaa actggatatg aacagcatgg cgcgacttta tacccgcgag
      361 attgacggta aaacgcatta ctacagtgtg gccccgcgtt tctgggtacc gtatgacacg
      421 gtgtacagcg tcgagaaaaa tgaagatcgc cggacagccg aacgctttca gaaatgggtg
      481 gaaatgggcg ttctgaccgt taccgatggt gcagaggtgg attatcgcta catcctcgaa
      541 gaggccaaag cggcgaacaa aatcagcccg gtcagtgagt cacccatcga ccctttcgga
      601 gcgaccgggc tgtcacatga ccttgctgat gaagacctga atcccgttac tatcgtccag
      661 aacttcgcca atatgtccga cccgatgaaa gagctggagg cagcgattga atcgggacgc
      721 tttcatcatg acggcaatcc catcatgacc tggtgtatcg gcaatgtggt cggcaaaaac
      781 atgccaggta acgatgattt agtgaagccc gtcaaagagc aggcggaaaa caaaatcgat
      841 ggtgcagttg cgctgattat ggcggttggc agagccatgc tgtacgagaa agaagacacg
      901 ctgtctgacc acattgagtc ctatgggatc cgctcgcttt aactgaggta attatgatca
      961 tgctgattct cgcgcctctg gtgggcgtgc tgggggcgct tttgctggcg tatggtgcct
     1021 ggctgattta tcccccggcg gggtttgttg ttgccggggc gttgtgcctg ttctggtcgt
     1081 ggctggtggc gcgatatctc gaccgtacac agtcgtctgt cggcggaggt aaatagtgtt
     1141 cttttctgga ttatttcaac gaaaaagtga cgcaccggtg accacgccag cagagctggc
     1201 ggatgccatc gggttgtcct acgacaccta taccggaaag cagatcagca gtcagcgggc
     1261 catgcgactg acggcggttt tttcctgcgt cagagtgctg gcagagtcgg tcgggatgtt
     1321 gccctgcaat ctgtatcacc tgaacggcag cctgaagcag agagccaccg gcgaacgtct
     1381 gcataaactg atctccacgc atcccaatgg ctatatgacg ccgcaggagt tctgggagct
     1441 ggtggtcacc tgtctgtgcc tgaggggaaa cttttacgcc tacaaagtga aagcatttgg
     1501 cgaagtggct gaactgctgc ccgtcgatcc cggctgtgtg gtaccgaagc ttaacagtag
     1561 ctgggagccg gtctatcagg tcacattccc ggacggctcc acggatgtgc tgagccagga
     1621 agatatctgg catgtgcgca cgctgacgct ggacggactg gtggggctga atcccatcgc
     1681 ctatgcccgc gaggcaatat cgctggcggc agcgaccgaa gagcacgggg ccagactgtt
     1741 cagcaatggt gcggtgacgt cgggtgtgtt gcgtacagag cagacgttgt cggatcaggc
     1801 ttacgagcgc ctgaagaaag attttgagga gcgtcacacc gggcttggta atgctcaccg
     1861 cccgatgatc cttgagatgg ggctggactg gaagtcgatg gcgctgaacg ccgaggacag
     1921 ccagttcctg gaaacccgca agtttcagct tgaagaaatc tgtcgtctgt tccgtgtgcc
     1981 attgcacatg gtgcagaaca ccgatcgcgc caccttcaac aatatcgaag agctggggct
     2041 gggatttatc aactattcac tggtgccgta tctgacccgc attgagcagc ggatcaacac
     2101 cggactggta cgaaaaagta agcagggcat ttattacgcc aaatttaacg ccggggcgtt
     2161 actgcgcggg gatatgaagt cccgttttga agcctatgcc accgggatca actggggaat
     2221 ttactctccc aatgactgcc gcgacctgga agatatgaat ccgcgtcccg gtggtgatgt
     2281 ctatctcaca ccgatgaata tgaccacgaa accctccgat ggcagtaaag ccggtaagca
     2341 gaaggataac gccaatgcag acgaaacaac gtcttgatgt accgctgagt ctgaaatctg
     2401 tcagtgactc cggtgagttt gaagggtatg gctccgtctt tggtgtaaag gacagccacg
     2461 atgatgtggt gatgtccggg gcatttgctg cttccctgcg ggcgtggagt gacagaaaag
     2521 cgttacctgc gctgctctgg cagcaccgca tggatgaacc catcggtgtt tataccgaaa
     2581 tgaaggaaga cgatgtcggg ctttacgtca ggggacggtt gctcattgat gatgatcccc
     2641 tcgcaaaacg cgcacatgca cacatgaagg ccggttcgtt aaccggcctt tctattgggt
     2701 acgtcctgaa agactgggaa tacgaccgga gtaaagaagc ctttctgttg aaagaaatcg
     2761 acctctggga agtcagtctg gtgacgttcc cgtctaacga cgaggcgcgg atcagcgacg
     2821 tcaagaacgc gctggcccgc ggtgaaatcc ccgaacagaa aaaaatcgaa agagtcctgc
     2881 gtgatgtcgg actctcccgt acccaggcca aagcattcat ggccgggggc tatggcgcac
     2941 tgtccctgcg cgacgctgag gatgtgggct ctgcactgaa tgcactgaaa aatctgaact
     3001 tctaatcagg agaaatacga tggcggttga tattaaagat gtggaacaga tcgcgcagga
     3061 gctgcagcag aagtttgacg acttcaaagt aaagaacgac aagcgcgtgg atgcgattga
     3121 gcaggaaaaa ggcaagcttg ccgggcaggt ggaaaccctg aacgggaaac tcagcgagct
     3181 ggaaaatctc aaaagcgacc ttgaaaaaga gctgcttgag ctgaaacgtc cggc
//
LOCUS       DBBGLQ010000251         3223 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.251, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000251 DBBGLQ010000000
VERSION     DBBGLQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.251"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..557
                     /locus_tag="ACIYSB_005420"
     CDS             <1..557
                     /locus_tag="ACIYSB_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGC5432225.1"
                     /translation="ALCVLRQYADEFSPYWYELGLLHFINGEPVKAATAFRRGFAENT
                     YIAEILCGNLYPFPLALWHDYSSEPDTARQYYETFCPLWGKYPEALLFVNWLYNQSGV
                     LSERAEFMKCAEMLLRADDQEMNESIFQQRELLRERIDDRLSEEIVQKLRTLNGEEIW
                     PWMLPFSGELIPADICFQNSLKYQ"
     gene            complement(659..1039)
                     /locus_tag="ACIYSB_005421"
     CDS             complement(659..1039)
                     /locus_tag="ACIYSB_005421"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGC5432226.1"
                     /translation="MIPVSPGQVRHTLVDTLTIAVCGVLVGADNFQEIELWAGNALSG
                     SASFLPLKNGIPSHDTFARLFGLIVPEQFEAAFRRWGSEVLPVLSSQVVAIDGKTSRR
                     SGSRCQSPLHRGNQSAMVYCSDLG"
     gene            1167..1277
                     /locus_tag="ACIYSB_005422"
                     /pseudo
     CDS             1167..1277
                     /locus_tag="ACIYSB_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1602..2258)
                     /locus_tag="ACIYSB_005423"
     CDS             complement(1602..2258)
                     /locus_tag="ACIYSB_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HGC5432227.1"
                     /translation="MTDNENILTPSKIIKFLDYAYEKSIDGIGIPGIDTAHSLAESYL
                     KTGSSLKEQINSLIRWQNTKSATSGFLTGLGGIITLPVTIPTNISSVLFIQVRMVASI
                     AIMCGQDIRDDKVRTIVYTCLVGNAAKDILKEAGIQIGQKLTTNAIRCISKDIIVKIN
                     KAVGFRLLTKTGATGVINMSKFVPVVGGIVGGSLDAITTNIVGNYARDTFLSLIDNDL
                     "
     gene            complement(2632..2985)
                     /locus_tag="ACIYSB_005424"
     CDS             complement(2632..2985)
                     /locus_tag="ACIYSB_005424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432228.1"
                     /translation="MGFWDAIMGFGKLASNAAAETMKKANFNVWDKIKSSPVERINDF
                     YNQNNTSEHNRPACRGLAIAALCFRGDWSGERLWREDQEAANWLKNFRIKISLDTCDS
                     ADELRKIIDRLTELN"
BASE COUNT          978 a          694 c          594 g          957 t
ORIGIN      
        1 gggctctctg tgttctccgg cagtatgcag atgaattttc gccttactgg tatgagcttg
       61 gtcttctgca ttttattaat ggagagccgg tgaaggcagc gacagctttc cggcgcggct
      121 ttgcagaaaa tacttatatc gcggagattc tatgcggaaa tctgtacccc ttcccgcttg
      181 ccctttggca tgattattca tctgagccgg atacagccag acagtattat gaaacattct
      241 gcccgttatg gggaaaatat cccgaggcat tgttatttgt taactggctc tacaaccagt
      301 ctggcgtact gagcgaacgg gctgaattca tgaagtgtgc tgaaatgctg ttaagggcag
      361 atgatcagga aatgaatgaa agcatttttc agcagcggga gctcttacgg gaaagaattg
      421 atgatcgtct ttctgaggaa atcgttcaga aattacggac attgaacggg gaggagatct
      481 ggccctggat gttaccgttt tccggagaac tgatccccgc agacatttgc tttcagaaca
      541 gtctgaaata tcagtaatat ctgcaccagg atgccattcc ggggtcagta aggatcttcc
      601 ccctgacctg gacaaatgta atgaaactca gctaagcccc agaagagcag gcatatagtc
      661 aaccgaggtc gctgcaataa accatcgcgc tttgattccc ccgatgaaga ggtgactggc
      721 agcgacttcc tgagcgacga cttgtctttc catcaatagc gactacctga gaagataaaa
      781 caggaaggac ttctgaaccc catcgccgga acgcagcttc aaactgctca gggacaatca
      841 gcccaaaaag acgggcgaat gtatcatggg aaggtatgcc attttttaga ggtaaaaaac
      901 tggcggagcc actcaatgcg ttgcctgccc agagctcaat ttcctgaaaa ttatccgcac
      961 cgaccagcac accacaaacg gcaatggtca atgtatcaac cagagtgtgc ctgacttgtc
     1021 cagggctgac agggatcatg tattgagatg aatatatcgg taaccggcgg tatatgagac
     1081 ataatatatc cctttgataa aaaaggttat gttgacatat ttatcctgca tgtcacgtat
     1141 cattattttt catgcgattg ccctgcggtt accaccgctc acagttagta aaggactggg
     1201 cggaagtggt caatataagg ttgcattacc ttcctcctta tagtccgaat ctgaatccga
     1261 ttgaacggat gtggaaaaat gctgcttaat agtattgatg ttcatcttta tgcctctaaa
     1321 agaaagcata agatcacaac aggctcagca ggtcaatcac tctatttttg ttcaaaaaac
     1381 gttcatgatc ttgccctacc caattttgtg ggtaaggata tgcgatgaaa ttctccttgc
     1441 agtttaatac aacctttcca tattaagcgg cagtattacc tgccagtcag gataatttta
     1501 cacctaatgt tagttaaaat ttgtgcaact tgaaacaata atactacagt atccactatc
     1561 gtcagcacac ctcaaatact attttttatg aaacgtttat tttataaatc attatcaatc
     1621 aaactcagga atgtatctct ggcataatta ccaacgatgt tggttgttat cgcatcaaga
     1681 ctaccaccaa cgattcctcc tacaacagga acaaactttg acatattaat aacaccagta
     1741 gcacctgttt ttgtaagcag tctgaaaccg actgccttat ttatctttac aataatatct
     1801 ttactaatac atctaattgc atttgttgtc aacttttgtc ctatctgaat tcctgcttcc
     1861 ttcaaaatat ctttcgcagc attacctaca agacaggtat aaacaatagt tctgacttta
     1921 tcatcacgaa tatcctggcc acacatgatc gcaatagatg caaccatcct gacctgaatg
     1981 aaaaggacac ttgaaatatt ggtcggtata gttactggta atgtaataat tcctccaaga
     2041 ccagtaagaa aacctgatgt ggctgacttg gtattttgcc atcgaataag gctatttatc
     2101 tgttctttta aactactacc cgttttcaga taactttccg caagactatg tgccgtatca
     2161 attcctggga taccaatacc atcgatggat ttttcataag cgtaatccag aaattttatg
     2221 atttttgatg gcgtgagaat attttcatta tcagtcatac aacaaaacac ctaaaaattt
     2281 cacgatcact ttctaaaaac tatctccgac agatatcgga acaccagtat tatcaccaat
     2341 taaataatat tattttttga gaaacaattg tattgctctg gcaaaaagat tttctgtaat
     2401 tgtagcctta cgtttataaa taactttctc tccacacaca ggacattcat aaaatcctgc
     2461 tgtacaattc atatgaataa tagttttact ccattgacaa tttgtacaga taaatgtaat
     2521 gggtggacag ggaaaaatca ttggctcaca ctctcgatat ataaataaca cttacaggga
     2581 catgtaaata ttttcgattt atccctataa aaataacttc acctccagta atcaattcag
     2641 ttctgtaaga cgatcgatta ttttacgtag ttcatctgca gaatcacatg tatccagtga
     2701 aatttttatt ctaaaattct ttaaccaatt agcagcctcc tgatcttcac gccataaacg
     2761 ctccccgctc caatcccccc taaaacatag agcagcaata gccaatcctc tgcatgctgg
     2821 ccgattatgc tcagatgtat tattttggtt ataaaaatcg ttaattcttt ctaccggaga
     2881 tgattttatt ttatcccaca cattgaaatt agcttttttc atcgtttcag cagcagcatt
     2941 agaagcaagc ttaccgaatc ccattatagc atcccaaaat cccatatatc accttatgct
     3001 atataatatc taaatgagta tctaatcttt tagaaccact atttggacaa tggttttacg
     3061 ccacttacat ctggtacaaa tgaatgtaac cgggggctga ggaagtgcca tgaaggtacg
     3121 tgtctgttgt attaaactat caaatcagac cacacgctgc gattttgaat ttaacaacgt
     3181 gtagtcagat attacattca gaagctgtat gtagcaccta acg
//
LOCUS       DBBGLQ010000252         3222 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.252, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000252 DBBGLQ010000000
VERSION     DBBGLQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.252"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            107..922
                     /gene="cma"
                     /locus_tag="ACIYSB_005425"
     CDS             107..922
                     /gene="cma"
                     /locus_tag="ACIYSB_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HGC5432229.1"
                     /translation="METLTVHAPSPSTNLPSYGNGTFSLSAPHVPSAGPLLVQVVYSF
                     FQSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAY
                     HFAKPAPSQYDYRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKI
                     NQIKDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYN
                     GVVRSYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(972..1325)
                     /gene="cmi"
                     /locus_tag="ACIYSB_005426"
     CDS             complement(972..1325)
                     /gene="cmi"
                     /locus_tag="ACIYSB_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HGC5432230.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
     gene            complement(1497..2306)
                     /locus_tag="ACIYSB_005427"
     CDS             complement(1497..2306)
                     /locus_tag="ACIYSB_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGC5432231.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVVAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVVARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(2307..2612)
                     /gene="ccdB"
                     /locus_tag="ACIYSB_005428"
     CDS             complement(2307..2612)
                     /gene="ccdB"
                     /locus_tag="ACIYSB_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HGC5432232.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(2614..2832)
                     /gene="ccdA"
                     /locus_tag="ACIYSB_005429"
     CDS             complement(2614..2832)
                     /gene="ccdA"
                     /locus_tag="ACIYSB_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HGC5432233.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
BASE COUNT          858 a          803 c          689 g          872 t
ORIGIN      
        1 caggcaaaca atgttatggg tagcctctct catggggttt gttcgccggt gtaagcgtta
       61 gccagccgcg aactcagtta tcttattaac ttataaggag ttatgtatgg aaaccttaac
      121 tgttcatgca ccatcaccat caactaactt accaagttat ggcaatggta cattttctct
      181 ttcagcaccg catgttccta gtgctggccc tcttttagtc caggttgttt atagtttttt
      241 ccagagtcca aacatgtgtc ttcaggcttt aactcaactt gaggattaca tcaaaaaaca
      301 tggggccagc aaccctctca cactgcagat catatcgaca aatattggtt acttctgtaa
      361 cgccgaccga aatctggttc ttcaccctgg aataagcgtt tatgacgctt accacttcgc
      421 aaaaccagcg ccaagtcaat atgactatcg ctcaatgaat atgaaacaaa tgagcggtaa
      481 tgtcactaca ccaattgtgg cgcttgctca ctatttatgg ggtaatggcg ctgaaaggag
      541 cgttaatatc gccaacattg gtcttaaaat ttcccctatg aaaattaatc agataaaaga
      601 cattataaaa tctggtgtag taggtacatt ccctgtttct acaaagttca cacatgccac
      661 tggtgattat aatgttatta ccggtgcata tcttggtaat atcacactga aaacagaagg
      721 tactttaact atctctgcca atggctcctg gacttacaat ggcgttgttc gttcatatga
      781 tgataaatac gattttaacg ccagcactca ccgtggcatc atcggagagt cgctcacaag
      841 gctcggggcg atgttttctg gtaaagagta ccagatactg cttcctggtg aaattcacat
      901 taaagaaagt ggtaagcgat aatataaggg ccgggattcc ggccttttta tacagccttt
      961 ataacagtgt attagtcatt cgcatattca acatatccct cctcacaatg gtacatgcca
     1021 taatacattc tcttaccctc cgggccttca gctttaaatg ggacaagaag tgttttttca
     1081 tcttcaacat ctgttaaact tctctcaatc ttttcccatg taattttcgg gatttctgta
     1141 ccaagaagtt gaacatcgtt gtcccagcgt ttcattcttt gcaggtaatc attttttaca
     1201 aacgttctgg cctgttcatc gctgacctga tagcatgccg gacctttatc ttcagaccag
     1261 aaaaaaacag cagcaagagc aataatcgtt aaaataaaaa taaatttcat gctaattact
     1321 ttcataaaat ctccacaact tctgtcgttc attcgatata aaaaacatta cgaatatatc
     1381 cgtacaatgt gagcatactg tgaagtggct ttaacaatta taaaataata caagagatca
     1441 catattgcag tggtctcttt tttatagaag aaatcacaat tcataattgt gacttattat
     1501 gcatgtttgt ttttcagcat tgtgaccgca tcggactcag gcatcgaaaa ctgcaccctg
     1561 tgccgtgcaa ccacatccag cgcaaacacc ttcgtgtaga cctccgttga gctgatggac
     1621 ttatgcccca tcaggctctg caggaccttc agcggtatac cggcatacag catgtgcatc
     1681 gcataggaat ggcggaacgt atgtggcgtg accgggacag agaacgtcac accatcagcg
     1741 gcagcggcga caaccgcttc cccaatccag gtcctgaccg ttctgtccgt cacttcccag
     1801 atcctcgctt tctctgtcct gcctgttcgt ttattacgcc gttccatggg gattttcagt
     1861 gttgccacca tcgtctgcag ctggctgacg taccaggagt cggagagcgg aaccagccgg
     1921 tgagtctgct gcccggcagg cgttcttcct gccgtcctgg cggctttttc tgtccgctgc
     1981 ttcagagtgg ccagctgcac aaacggatac ggaggcgcga gcgaaaaatc cccccgcgtc
     2041 agcgccagtg cttcattaat gcgcgcaccg gtgttccaca gtgtggccag cagcatcttg
     2101 cggcgcagat ccgggacgta atggagcagg gcactcactt caggggcgag caggtatttt
     2161 ggcagttcat catgaaccat cgacatctgg cggagtgcca gcgcagccgg ataatcaatg
     2221 gcaacaggca gcgatgcagg ctgtccggca gaatacactg ccggtaccat ggcagcagac
     2281 tggctgtgta taacggagcc tgacatttat attccccaga acatcaggtt aatggcgttt
     2341 ttgatgtcat tttcgcggtg actgagatca gccacttctt ccccgataac ggagaccggc
     2401 acactggcca tatcggtggt catcatgcgc cagctttcat ccccgacatg caccaccggg
     2461 taaagctcac gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc
     2521 cgtcgcccgg gcgtgtcaat aatgtcactc tgtacatcca caaacagacg ataacggctc
     2581 tctcttttat aggtgtaaac cttaaactgc atttcaccag tccctgttct catcggcaaa
     2641 agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt
     2701 ccagcgttcg gcacgcagac gacgggcttc attctgcatg gtggtgctta ccagaccgga
     2761 gatattgaca tcatatgcct tgagcaactg atagctgtca ctgtcgacgg tgactgtaat
     2821 gcgctgcttc ataatgcacc tcatcttaca tactctctgt acacaattaa gtatacacca
     2881 gtattacgct ataacatgcc tccggcaacg gaatattatt atctgactat tattaatgct
     2941 ttcatcaata accgatctaa aaagtgacca tgacaataat aaccagcaat ggtagtaacc
     3001 atgccaaatt acagaataac tggttttcca tgatccgatt tctatcggat tcaggaattt
     3061 ccggaagtga atcacgatca gtgaataagc gaagtgatct tttctgccgc tgatgactgg
     3121 ctcgcaaaca cgggaataaa cacaacatac agtgttatgt cagcgggtat gaacacaaca
     3181 tcctgtgaaa tctggccggc ggacggcagc gaaggatccg cg
//
LOCUS       DBBGLQ010000253         3207 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.253, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000253 DBBGLQ010000000
VERSION     DBBGLQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.253"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(60..587)
                     /gene="mobB"
                     /locus_tag="ACIYSB_005430"
     CDS             complement(60..587)
                     /gene="mobB"
                     /locus_tag="ACIYSB_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGC5432234.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(569..1153)
                     /gene="mobA"
                     /locus_tag="ACIYSB_005431"
     CDS             complement(569..1153)
                     /gene="mobA"
                     /locus_tag="ACIYSB_005431"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGC5432235.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMVF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1223..1492
                     /locus_tag="ACIYSB_005432"
     CDS             1223..1492
                     /locus_tag="ACIYSB_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGC5432236.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1569..2555
                     /gene="srkA"
                     /locus_tag="ACIYSB_005433"
     CDS             1569..2555
                     /gene="srkA"
                     /locus_tag="ACIYSB_005433"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGC5432237.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2572..3198
                     /gene="dsbA"
                     /locus_tag="ACIYSB_005434"
     CDS             2572..3198
                     /gene="dsbA"
                     /locus_tag="ACIYSB_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGC5432238.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          811 a          773 c          784 g          839 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc ggatgcgacg ctggcgcgtc ttatccagcc tactcttgct
       61 tatccgttct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta atgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc aggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaactgtag atccagctct tcttcgtctg gtgtttccgt catcaaggcc
      361 catcgttgct ggctggcaac gatggtttgt gccgcgccag ccttgcgcag ctcatagcta
      421 tctttgcctg gcttatcaac atccatatca tggtgcgtat gcttaatcag ccctggacgg
      481 atccctctgg cgcataatgc cgggatcagt tttttcaaaa gcgtagtttt tccggtacca
      541 ctccacgcgg caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgttaaca aatgcatctt tatgatcgct gaaatcaacc gcatgaccgc
      661 cagccagacg cataaatacc attacccggc gttctcctgc ttgcagatat tccagtaata
      721 aaggctcaat agcgcggttt accagagcaa tagtcgggtg atcgcgttca ccgtcatgga
      781 cccacacgac aggcgcatct ttgcgctgat gattaagcct ggctgctaaa tcagggggaa
      841 tgtaaggcgt atcgcacggg caaaacaaaa accactcacc agcttcctgc tgcattactg
      901 aaagcattcc tgccagaggg cctgggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttgcctg atagatttcc tgatgacgat tagcattaac cacgacgtga gagagctgcg
     1021 tcataagcgc gtcagcgaca tgttgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcctgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatca ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgctg caatttttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagacttc aaaacggcgc tgttacgcgc tcgcggcgta attaaagagt aaaagcttgt
     1501 aagcggcgcc accaaaatca tcgtgaaatg atatccttcg tcattcgtaa tgttttccgg
     1561 atgatgggat gaataacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcatggg atccgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag atcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 taaatgatga agttccggtc gcagcacctg tggcctttaa cggtcagact ttattgaatc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gatggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcgca
     1981 aacagctttt tatccatcgc ccgaccatcg gtttgaacga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctgatacctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gattgccgcc gttacagcac actggcggga agatttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagccgttc aggatttgtg gatgttgctc aatggcgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaagc ttatgaagaa tttagcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggctg cgacagacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttgcaatta acacctatgt attaatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccggtagctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgccc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aatgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 ggtggtgacc tgggcaaaga tctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tgactgttcc gctgtttgaa ggcgtacaga aaacccagac cattcgttct
     2941 gcttctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt gaaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggcgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggtatggata ccagcaatat ggatgttttt gttcagcagt atgctgatac agtgaaatat
     3181 ctgtccgaga aaaaataatt caatgta
//
LOCUS       DBBGLQ010000254         3137 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.254, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000254 DBBGLQ010000000
VERSION     DBBGLQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.254"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            317..1291
                     /locus_tag="ACIYSB_005435"
     CDS             317..1291
                     /locus_tag="ACIYSB_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HGC5432239.1"
                     /translation="MSEDKFLSEYSPKDSVWDSHRAFTDSVGGIYRSAAEFERYAERM
                     ASCSGLLRFGWSTLMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     ADYPSSRWLFLTLTVRNCAIGELGDTLTTINAAFKRLEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVPPSWFSGVAYVKQARWVELWRDCLRVNYEPNVDIRTVKTKTGE
                     AVANVAEQLQSAVAETLKYSVKPEDMANDPDWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVMADGVNEGEDDGSRLAFSWETEIKKYRRDPKRDNPKSEG"
     gene            complement(1932..2102)
                     /locus_tag="ACIYSB_005436"
     CDS             complement(1932..2102)
                     /locus_tag="ACIYSB_005436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432240.1"
                     /translation="MTPQQSALKAASHKASQKIVARDQRSDDRFVCLTASFSNVSMPF
                     RRVRFTRTATPE"
     gene            2800..3051
                     /locus_tag="ACIYSB_005437"
     CDS             2800..3051
                     /locus_tag="ACIYSB_005437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432241.1"
                     /translation="MCNRITSHARQYDYRLVRAAPRHPANASRHAKKFRVPLTVPELV
                     KQKIAGSRASGVGSDFVIDDGTGRYWWWWLVSRSEANHL"
BASE COUNT          772 a          753 c          885 g          727 t
ORIGIN      
        1 aaaaaaatag ctcgaaattt gagcaattct cgcgcgcgat ccttgtattt atacttaagg
       61 gataactggc ggacgtaaaa tggtgcttca agccagtatt ggcgcggctt agagtggtgt
      121 ttgacggatc aaaggatttg gcgcaaaatt gaagtgtttt taagcggcgc tgaggagtgc
      181 gctaacactc ctcagcattt gaaagtgtca ccggaagatt agccgtcaga cggtgacagt
      241 aactggcacg caatgcacag gtacgtgatg aatataacac atcaaatcac cacctgcaca
      301 gatcggagca ggtggcatgt cagaagataa attcctgtcg gagtacagcc cgaaagattc
      361 ggtctgggac tcacatcgtg cgtttactga ttctgtcggt ggtatttacc ggagtgctgc
      421 tgaatttgag cgttatgcag agcgtatggc ctcctgtagc ggtttattgc gttttggctg
      481 gtctacgctc atggaaaccg gagagacgcg cctgaggctt cgtagtgcgc agttctgccg
      541 tgtccggcat tgtcctgtct gccagtggag aagaacgctc atgtggcaag cccgttttta
      601 ccaggctcta ccaaaaatcg ttgcggatta tccatcttca cgttggttgt tcctgacgtt
      661 aactgtcagg aactgcgcga tcggggaact gggcgatacg ctgactacga taaatgcggc
      721 gtttaagcgg ctggaaaaac ggaaggagct ttccccagta cagggctgga ttagagcgac
      781 ggaagttaca cggggaaaag acggcagcgc gcatccgcac tttcactgtt tgctgatggt
      841 gcctccatca tggttctctg gtgttgctta tgtcaaacaa gctcgttggg tggaactctg
      901 gcgggattgt ctgcgggtga attatgaacc gaacgttgat atccggacag tgaaaacgaa
      961 aacaggcgag gctgttgcca atgttgcaga acagctacag agtgctgttg ctgaaaccct
     1021 gaaatattcc gttaagccgg aagacatggc taatgatcct gactggtttc tggaactgac
     1081 gcggcagctt cacaagcgcc gtttcatttc gaccggtggc gcactcaaga acgtcctaca
     1141 gcttgacagg gaaaccaatg aggatcttgt gatggcggac ggtgtgaacg aaggagagga
     1201 tgacggctcg cggctggcgt tctcatggga aacggaaatc aaaaagtacc gacgtgatcc
     1261 gaaacgggat aacccgaaat cagaggggtg agcgcgtcgt tgtgaccccc catgttatag
     1321 ccaacacccg aacaggccac gcagacgcgc tcagagtgcg tctgagcgcg tttcagggtc
     1381 gcggcccctg aaaacgtgat tgcagacgtt tttgctcgcc agaaacgcgc tcaggcgttt
     1441 tttaacgcat agcgggtgcg gtgtcgagaa gggggttttt aaaacggaat ccgggtgccg
     1501 gaccgggggt gtcagaaaac accggttcgg atatgggtga agaacggagt tcgggaacct
     1561 ttgccggaca ttcgcaagaa aaacaacagg tttaatcttt taaaagatgc acaacgccct
     1621 ccgtttgaac gaaaacaccc aaagtacgtt tctttgttca aacagattaa cacattgata
     1681 ttgttagttt tgcgtcatca cggacggctc tgtaacgcgg ttgctcgcct tcctggctcg
     1741 tttgatgtcg atgtagcaga gcttgcacat gttgagtttt tacaggagtt cgctacctgt
     1801 gcaccagggc gattttttac tccccgtcat cttacgatga cgccttccga catcagaacg
     1861 atttttcata tttattcctt tatttcaaac agttaataca cttacctctc atgtattacg
     1921 aagtggaaaa ttcattccgg cgtggccgtc cgtgtaaaac ggacgcgccg gaaaggcatg
     1981 gacacgttac tgaaagacgc tgtcagacag acgaaacggt catcagaacg ctggtcacga
     2041 gcgacgattt tctggctggc cttatgtgat gctgctttca gggcagattg ttgtggcgtc
     2101 acttggtgtt ccctacggtc gcacacaagt gacgccgcac agcttcagac tccacgtaca
     2161 cacgcaagca gccaaatttc aaatggcctt atgccgtgca cacagtcaca agaagctaat
     2221 gcagctagac agaaaagttt taagacgcag atatataagg cattctcgcc actctgcgag
     2281 gggaaaactc gtaagtttca tgacacacaa aaccgtacgg gtaatccgaa ggtaaaaata
     2341 acgtacctca atcaccagtg gtgatatccg ttcccagagg ctaaacggat gtaccgccat
     2401 aaaggcatcc cctttcaggc tacgaggata ttgcaatcac ccgaaggcgt ttctctcaga
     2461 tcgctgaaac attggctggc agtcacccga aggcgctcct ctcagactgc tggaacattg
     2521 gctggtcatc cgaagatgac agtgtggtcg cgtaagcctc cctctcagac caccgggaga
     2581 ttggttggtc gccgaagcga caagttagcg tttaaatttt aagcacaaaa agtgatgcca
     2641 agcaatacgt taagcggctg cggtgcgtgc ggaactgcgc caggcagtgg aacaaccgac
     2701 gaccacgacc gcgccaccgg aacggacggg tcgggaacgg tgacgggcct gcgttctgcc
     2761 ttggtgcggc cgaccgtgac ggggccgcag cgggacagga tgtgtaatcg tataacctcg
     2821 cacgcacgcc aatatgatta ccgcctggtc agggctgcgc cccgacaccc cgccaacgcc
     2881 tcacggcacg cgaaaaaatt ccgcgtcccg ctcaccgttc ccgaactcgt caaacagaaa
     2941 atcgcggggt cccgcgcctc cggcgtgggt tccgattttg tgattgacga tggcacggga
     3001 agatactggt ggtggtggtt ggtgtcacgg agtgaggcca accacctgtg aggctgtttt
     3061 tgttgttcac aaattcgggg tgaaagtctg tggatgagat gggtgtaagt gggtgggatg
     3121 tgtggctttg gggggct
//
LOCUS       DBBGLQ010000255         3081 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.255, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000255 DBBGLQ010000000
VERSION     DBBGLQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.255"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..931)
                     /gene="mobF"
                     /locus_tag="ACIYSB_005438"
     CDS             complement(<1..931)
                     /gene="mobF"
                     /locus_tag="ACIYSB_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HGC5432242.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVS"
     gene            complement(931..>3081)
                     /gene="traD"
                     /locus_tag="ACIYSB_005439"
     CDS             complement(931..>3081)
                     /gene="traD"
                     /locus_tag="ACIYSB_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HGC5432243.1"
                     /translation="MRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIY
                     WWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVV
                     ALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGD
                     LPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSI
                     DKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYL
                     MRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
                     IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRG
                     LLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGE
                     KAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGK
                     DMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARPKVAPEFIPRDINPEME
                     NRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQQPQQP
                     VSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQ
                     ENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT          670 a          923 c          747 g          741 t
ORIGIN      
        1 gtgaaacagc cccgggcgcc tgcgtgcggg tctccgcacg ctgatccgcc gcgtcacgat
       61 atgcccggat gtcgaacccg gtttccttca gcgtctgcat ccattcagcc attctgactt
      121 caggatcgac gtgctgtttg gattttcgcg tatccagtgc cgccacatcc cgggatttca
      181 gcgaggcatc ttccccgacg gcctcccgga ttgcctgtga gcgtccggaa aaggcctcca
      241 ccggtacgcc cggcatctcc cacataccgt gcttacccac cacttcggtt tcatagccca
      301 gcgcctcaac ctgctctttc agtttttccc ggtagagcct gccaaaggca atctgattag
      361 cgtacacatt ctcaatgaac cccgttttcc ccactttgtc actgctcagt gtcttccatt
      421 cgccgttatg ctgcgtgaca ttagccacca ccgcatgcgt gtgtaactgt ggttcctgat
      481 cgcgactggt gtcgtggtta aacagtgcca tcaccagatt accggtcagc accgtttctg
      541 actgtccgtc cgtcatcacc cgtgtggagg ccagcgcctc cacctgacga acagcaaagt
      601 ccacggcctg gttatgtgca tcaatcaggc gcttatcgcc acctaacatg gccatcatgg
      661 agacactttt gggggcggag aaggtcaggt cgtagccggg acgatgcctg ttactgccat
      721 cctgcatgcg gcttagatcc gctccgtccg gcagccttcc ctccagaaga cgggtaaaaa
      781 catccttatc gacactgccc tgcagtccca gctgttcagc ccccctgccg gcccagcgtt
      841 ctcccatgct gcccagcaca tagtaattat ccttgtcggt ataatagtta ccggcacttc
      901 cggccgatct gacctgcgcg atactcatca tcagaaatca tctcccggct caacatcctc
      961 cccgcgctcc cggtgcacat taatgttcac ctcttcacga cgctgcatct gctgctggat
     1021 gtccggatga ttttcctgtt gccatgcctc ataagcggcc atatccacca cttcaccgga
     1081 ttcactgatc ccgggtggca gttgctgttc catctcttct tccggtttca ttttcagctc
     1141 ctgctcgatc ccccctgccg gaacattcac acctgaatct gacttcttat cgttgatggc
     1201 aggagacacc ggctgttgcg gctgttgcgg ctgttgcggc tgttgcggct gttgcggctg
     1261 ttgcggctgt tcagcctgag tcacgtcttc tccggaaaca acttccggca catccggttc
     1321 gaagaggctg gccatctgac gaccttctgc ttcccttgcg gcaagtacgg cactcagacg
     1381 gttctccatt tccgggttga tgtcacgcgg aataaactcc ggagcgacct tcggacgtgc
     1441 ctgatatttc agtgagagtt ttactgccgg atacggtccg ggcagggtga cataacaggt
     1501 caaatccggc agagactgaa tgtcagaata actgaccagc gtctggcgct ccatatcctt
     1561 accggtcgat accccgtcac gtaccggatc agcaccgtag gagtactgct cgctggcttt
     1621 caggtgctct ttctcaccaa tctcaccggc agcgaactct gcaatcttat ggctgggaga
     1681 acggaaaaag gcacgggtgt tcatgacgtc aaacagcgtg gcagccgctt tctcaccgta
     1741 gatatcttcc agctgggcat aggactggat accaaacaca taacagccac cgaacttacg
     1801 ggcttccggc aggatctcca ccaggtccgg cagtttgtgt aacgtgggta gctcatcaca
     1861 gaaaaaccac acacgacggt tacggttttc tcccattgcc agcagaccac gaatggcaat
     1921 ggacagccac atggagatca ccggcttcag ggaggcatgg gtgtcggcat tcgacgagat
     1981 aaacagccag ccgtttttct gatcttcccg gacaccccgc atccagtcac ggatggtaaa
     2041 agactcaccg ttatgctcaa tcccctgcag gtaacggatg gctttcacgt agttggtcag
     2101 cacagcacgg atggaaatcg ccgttttctc aattttctct tccaccaggt tggccgccgg
     2161 tgaattacgc aggaaggtac gcagtttttc gattttgatg gaaagcagtg tgtccaccag
     2221 tttgctgtag ctgcggttgg ggtcattacg catcaggtac gccgcttccg caaaaatggt
     2281 acgtcctgaa ccctgccaga acgggtcctc ttttgtgccc atcgggatca gggtatttgc
     2341 cgtattatca aaatccggct gtgtcagaca ctccttccat aaatcccagg cggcacagcg
     2401 ggcatccagc ggattcagga tcttatcgat ggagggatca tagtaacttt taacaaattc
     2461 ccctgaacgg tcataaatca ccaccatatc accgcgctga cgggcgtagt tggccagacg
     2521 acggataact tccgacttac cggccccgac cgtgccgtgc agacagaagt tctggatttc
     2581 agaatcccgg ataatcggca ggtcgccaat ccggatatcg gagtccttgc cgtctttttt
     2641 cagcatccgg gcaacgtctt tcggattgtc tgtcagctga cgaccacctg tgacttcatt
     2701 ttcgctctgt tgtttaccct gacgccccag aatccaggag acaacaaaga aggtaatcag
     2761 gcaaataacc agtgccacaa ctgtggccag aacgaatgcg gaccatagct gctctccgca
     2821 ccagatcata tatttatcat gcagtacctg agcagcattc atccggaagg ttttgccgta
     2881 atactggatc tcatagaccg gctgggactt gattaaatcc cgcatgcctt ccagcgtggt
     2941 acaccaccag taaatacagc cgttcacaaa cgtctgccag cttattttta cccataaaac
     3001 cagaccaacg agtatccaga aaaaaataaa caggcagtaa agcatgatat tggcgatctg
     3061 gctgaacatg cggatacgca t
//
LOCUS       DBBGLQ010000256         3061 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.256, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000256 DBBGLQ010000000
VERSION     DBBGLQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.256"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(19..171)
                     /gene="mokC"
                     /locus_tag="ACIYSB_005440"
     CDS             complement(19..171)
                     /gene="mokC"
                     /locus_tag="ACIYSB_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGC5432244.1"
                     /translation="MKQHKAMIVALIVICITDVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            757..1923
                     /gene="nhaA"
                     /locus_tag="ACIYSB_005441"
     CDS             757..1923
                     /gene="nhaA"
                     /locus_tag="ACIYSB_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGC5432245.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGARRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1989..2888
                     /gene="nhaR"
                     /locus_tag="ACIYSB_005442"
     CDS             1989..2888
                     /gene="nhaR"
                     /locus_tag="ACIYSB_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGC5432246.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2926..>3061)
                     /locus_tag="ACIYSB_005443"
     CDS             complement(2926..>3061)
                     /locus_tag="ACIYSB_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGC5432247.1"
                     /translation="NYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN
                     "
BASE COUNT          710 a          696 c          763 g          892 t
ORIGIN      
        1 cactccccgc cgttgctctt actcggattc gtaagccgtg aaaacagcaa cctccgtctg
       61 gccagttcgg atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacgtc
      121 ggtgatacag atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt
      181 ctccttgacc ttacggtcag taagaggcac tctacatgtg ttcagcatat aggaggcctc
      241 gggttgatgg taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga
      301 gacagacagc ctcaagcacc cgccgctatt atatcgctct ctttaaccca ttttgtttta
      361 tcgattctaa tcctgaagac gcctcgcatt tttgtggcgt aattttttaa tgatttaatt
      421 atttaacttt aatttatctc ttcatcgcaa ttattgacga caagctggat tatttttgaa
      481 atattggcct aacaagcatc gccgactgac aacaaattaa ttattacttt tcctaattaa
      541 tccctcagga atcctcacct taagctatga ttatctaggc ttagggtcac tcgtgagcgc
      601 ttacagccgt caaaaacgca tctcaccgct gatggcgcaa attcttcaat agctcgtaaa
      661 aaacgaatta ttcctacact ataatctgat tttaacgatg attcgtgcgg ggtaaaatag
      721 taaaaacgat ctattcacct gaaagagaaa taaaaagtga aacatctgca tcgattcttt
      781 agcagtgatg cctcgggagg cattattctt atcattgccg ctatcctggc gatgattatg
      841 gccaacagcg gcgcaaccag tggatggtat cacgactttc tggagacgcc ggttcagctc
      901 cgggttggtt cactcgaaat caacaaaaac atgctgttat ggataaatga cgcgctgatg
      961 gcggtatttt tcctgttagt cggtctggaa gttaaacgtg aactgatgca aggatcgcta
     1021 gccagcttac gccaggccgc atttccagtt atcgccgcta ttggtgggat gattgtgccg
     1081 gcattactct atctggcttt taactatgcc gatccgatta cccgcgaagg gtgggcgatc
     1141 ccggcggcta ctgacattgc ttttgcactt ggtgtactgg cgctgttggg aagtcgtgtt
     1201 ccgttagcgc tgaagatctt tttgatggct ctggctatta tcgacgatct tggggccatc
     1261 attatcatcg cattgttcta cactaatgac ttatcgatgg cctctcttgg cgtcgcggct
     1321 gtagcaattg cggtactcgc ggtattgaat ctgtgtggtg cacgccgcac gggcgtctat
     1381 attcttgttg gcgtggtgtt gtggactgcg gtgttgaaat cgggggttca cgcaactctg
     1441 gcgggggtaa ttgtcggctt ctttattcct ttgaaagaga agcatgggcg ttctccagcg
     1501 aagcgactgg agcatgtgtt gcacccgtgg gtggcgtatc tgattttgcc gctgtttgca
     1561 tttgctaatg ctggcgtttc actgcaaggc gtcacgctgg atggcttgac ctccattctg
     1621 ccattgggga tcatcgctgg cttgctgatt ggcaaaccgc tggggattag tctgttctgc
     1681 tggttggcgc tgcgtttgaa actggcgcat ctgcctgagg gaacgactta tcagcaaatt
     1741 atggtggtgg ggatcctgtg cggtatcggt tttactatgt ctatctttat tgccagcctg
     1801 gcctttggta gcgtagatcc agaactgatt aactgggcga aactcggtat cctggtcggt
     1861 tctatctctt cggcggtaat tggatacagc tggttacgcg ttcgtttgcg tccatcagtt
     1921 tgacaggacg gtttaccggg gagccataaa cggctccctt ttcattgtta tcagggagag
     1981 aaatgagcat gtctcatatc aattacaacc acttgtatta cttctggcat gtctataaag
     2041 aaggttccgt ggttggcgca gcggaggcgc tttatttaac tccacaaacc attaccggac
     2101 agattcgagc gctggaagag cgcctgcaag gcaaattatt taaacgcaag ggacgtggtc
     2161 tcgaacccag cgagctggga gaactggtct atcgctatgc cgataaaatg ttcaccttaa
     2221 gccaggaaat gctggatatt gtgaactatc gcaaagaatc caatttattg tttgacgttg
     2281 gcgtggctga tgcactttcc aaacgcctgg tcagtagcgt acttaacgcc gcagtggtag
     2341 aaggcgagcc cattcatctt cgctgcttcg aatccaccca cgaaatgctg ctggagcaat
     2401 taagtcagca taaactggat atgatcattt ctgactgtcc gatagactct acgcagcagg
     2461 aaggcctgtt ctccgtgaga attggcgaat gtggcgtgag tttctggtgt acaaatccac
     2521 caccagaaaa accgttcccg gcttgtctgg aagaacggcg acttttgatt cctgggcgac
     2581 gttcaatgtt agggcgcaaa ttgcttaact ggtttaactc ccagggatta aacgtagaaa
     2641 tcctcggcga gtttgatgat gccgctttga tgaaagcttt tggtgcgatg cacaatgcaa
     2701 tcttcgttgc cccaacgctt tatgcatatg acttttatgc cgataaaact gtcgtagaaa
     2761 ttggtcgcgt cgagaatgtg atggaagagt accatgctat ttttgctgag cggatgattc
     2821 agcacccggc ggtacagcga atctgcaata cggattattc tgcgcttttt agtccagcgg
     2881 tgcgttaatc ggcagctccc ccaaagttaa ggtgggggag atagattagt tgtacattac
     2941 cacgattttg actcggctca ttatttgccc gcttgagaca ttgtttccat atgtacgcgg
     3001 gcgaataaat agaggaatct gattacttcc ttcatgggga tgctgaaaag agtagtaatt
     3061 g
//
LOCUS       DBBGLQ010000257         3054 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.257, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000257 DBBGLQ010000000
VERSION     DBBGLQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.257"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            610..1587
                     /locus_tag="ACIYSB_005444"
     CDS             610..1587
                     /locus_tag="ACIYSB_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGC5432248.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(1872..2612)
                     /locus_tag="ACIYSB_005445"
     CDS             complement(1872..2612)
                     /locus_tag="ACIYSB_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5432249.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(2733..2921)
                     /locus_tag="ACIYSB_005446"
     CDS             complement(2733..2921)
                     /locus_tag="ACIYSB_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HGC5432250.1"
                     /translation="MESEIFSCLLRASAGFFLRFCLSWHYPNSPKNGKPALSPHHDWL
                     KTGSQRQNAPLRIWFYPQ"
BASE COUNT          775 a          767 c          763 g          749 t
ORIGIN      
        1 agtgaaaccg atattgtaca gaaagtacag taattacagt gtgttacctg cagagaggct
       61 atcagatttc tgctccggta tagcaatcaa aaaaacagtg ctcgccgcag accggtgacc
      121 gggcgaagcc cgcgaaccgg cgaggaagcg caatgtatct gtagttacag tgagcaggta
      181 cgccctgaag ccctttcata aaacggttgt gtgcgctgtc cttctggaat gatttatcca
      241 catatccgga ggcccgatcc agtaaacaga tccatgaatg atcaacaaag gatccattaa
      301 agatccccat atcgctgcaa accttgtcac tcatgggccg ggaccacgat cacataagct
      361 gtggcatgtt actgataaac tgtaacatgc taatgataag ctgtattcag taatccatat
      421 actgaagtaa gttaatgaca taaactatgg tcagtatgcc agactcagtt gttaaataca
      481 ggctgcaggt ttttcttcag tcagttagcg gagttctgac acacgatttt ctgtttattc
      541 ttttactgtc cacaggctgg aggctttctg gaaaacgaaa attcagacat caaaaaactg
      601 ttcggcgagg tggataagtc gtccggtgaa ctggtgacac tgacaccaaa caataacaac
      661 accgtacaac ctgtggcgct gatgcgtctg ggcgtttttg taccgaccct taaatcactg
      721 aagaacagta aaaaaaatac actgtcacgt actgatgcca cggaagagct gacacgtctt
      781 tccctggccc gtgctgaggg attcgataag gttgagatca ccggcccccg cctggatatg
      841 gataacgatt tcaagacctg ggtggggatc attcattcct ttgcccgcca caacgtgatt
      901 ggtgacaaag ttgaactgcc ttttgttgag tttgcaaaac tgtgtggtat accttcaagc
      961 cagtcatccc gcaggctgcg tgagcgcatc agcccttccc tgaagcgcat tgccggtacc
     1021 gtgatttcgt tttcccgtac cgatgagaag cacacccggg aatatatcac ccatctggta
     1081 cagtcagctt actacgatac tgagcgggat attgtgcagt tacaggccga tccccgcctg
     1141 tttgaactgt accagtttga cagaaaagtc cttctccagc ttaaggcgat taatgccctg
     1201 aagcgacggg agtccgccca ggcactctac acctttatag agagcctgcc ccgggatccg
     1261 gcaccgatat cgctggcgcg gctgcgtgca cgcctcaatc tgaagtctcc tgtattttcc
     1321 cagaaccaga cggtcagacg ggcaatggag cagttgcgcg agattggata tcttgattac
     1381 acggagatcc agcgggggcg gacaaaattc ttctgtattc actaccggcg tccccggtta
     1441 aaagcgccga atgatgagag taaggaaaat ccgttgcaac cttcacctgc ggaaaaagtc
     1501 agtccggaga tggcggagaa gcttgccctg cttgagaaac tgggcatcac gctggatgac
     1561 ctggaaaaac tcttcaaatc ccgctgaaca taaactgtag tcagtgaaga gcgctccttt
     1621 actgactaca gcttatatta accggtgcag tgagtggtct gctcactgca gtttatattc
     1681 tgtttcctgc agtgctgtct gtagctgagc tgccatctgc ctgtccctta cgtaagtgat
     1741 cccgtaacct gatgctgagg cattgctccc ttcataaaac atgacttact cactacagct
     1801 tatatacatg ctccagctta tgttatgtct gttctgctga ccacagctta tataaggaag
     1861 cgcgtatgcc gtcacctcag gggaggcagt gtacgcagga tctccgcagc atcccgcccg
     1921 tcaccggtga aaggcactgc cagcgtggca gccatatcca gtgcaaacac tctggtataa
     1981 acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
     2041 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
     2101 gaaaagtgta ctccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
     2161 gtttcgtcgg tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
     2221 ctttccatct gcctgacata gcttatatct gtcagcggaa ccaggcgcac ttcatctttt
     2281 ggcgggcgtc cgcgtcgcgc acgcactttt tcggacagga tccgcacaaa cggtcttact
     2341 ccattcaggt caaatgattc cggcgtcagc atccgggctt cgccaatacg cattccggta
     2401 ttccagaggg tggcgaacag catatggtga cgctgatccg gcatatagaa aagaagggca
     2461 ctcacttccg gggccagcag gtaggccggt gtggcccccg tggaagtggc cattcttctc
     2521 agggagagcg ctgttgcaaa atccaccccc ggcgcaatgg gtaacaggga gacgcgttct
     2581 ggtgaattct gcaggggaat gacattgttc atgagttatt tttcacgtag cacacgcact
     2641 gtttatggat acaggtattc tgacctgaac gcccggatcc ttccacgaca ataaatcgca
     2701 gcgcgcggat ccttttgttt gattatcacg cattattgcg ggtaaaacca aatacgtaaa
     2761 ggcgcatttt gccgctggga tcccgtctta agccagtcat ggtgcgggct gagggctggt
     2821 ttaccgtttt tgggagaatt ggggtaatgc caggacaggc agaaacggag aaaaaatcct
     2881 gcagacgccc ttaaaagaca actgaaaatt tctgactcca tattcctctg gtttcagact
     2941 gcttaatcac atcatcaacc tgatacataa caaacagatt ctgttcacag aatgaacaat
     3001 taagtaaata gttagattat gcgaaaaagt tacagatatt tctcaccatc gtta
//
LOCUS       DBBGLQ010000258         3028 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.258, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000258 DBBGLQ010000000
VERSION     DBBGLQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.258"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            489..1280
                     /locus_tag="ACIYSB_005447"
     CDS             489..1280
                     /locus_tag="ACIYSB_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432251.1"
                     /translation="MTLRINDQGRDKQNQFLSKNGENLNSLKSRHVQDRHLPEWLKDK
                     VAAFWFCLVIKDMKVSGSSYYELTMQLPTSEESVCISKLSSSTHEIRLEIIRRWIDSV
                     AQIETEAFTKNLVDAIYKNWKEIGNKNQATWLKKNPEQLEWAWKYINIRIPESHLTWF
                     CPVNNEEKRIAILALLKILYPGDIPCQYNDKEFHVGICAEEHFYRRMHDAFRKQFIDG
                     KKDKRVQINVKISPSAKSALDRLTRARKTTQQAILEQLILNGRLD"
     gene            complement(1277..2263)
                     /locus_tag="ACIYSB_005448"
     CDS             complement(1277..2263)
                     /locus_tag="ACIYSB_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432252.1"
                     /translation="MTAQQSDALREIANKARVTTILQSKAWKDTQRILKRSGLVCRER
                     SEPFDPEKHFDCYTVRYLYLLNIIALELRPDTRPDTRPDTRIKVEVGHWYRMTGKRLS
                     LNVPPFMLIPRNIRRKVDGFRQSEGKTTKKPTQPFTGSLYEVLSGDIDSAKLDAWIAG
                     LPLSALEGAKEIPIMGFSPWVVAKSVRQSASPTFELFYQEYQSLVLKSVFKLEPGFEQ
                     LLTRLSFIKQSILSKQQYRVKSQLLERLGNVLFFTLLYNQGCPGEFINTLVEKEDIYL
                     KASPGEEKVKAHQKLINYIEEFCNEMTEKYLMPAAGRHYKKRKTARSGSSES"
     gene            2370..>3028
                     /locus_tag="ACIYSB_005449"
     CDS             2370..>3028
                     /locus_tag="ACIYSB_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjP family GTPase"
                     /protein_id="HGC5432253.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSHICATHPLNGCTRQAHHLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRALYHQLLPELDLIIWILRADERAYAADIAMHQFLLNEDADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLQALVSLMI
                     HA"
BASE COUNT          833 a          734 c          624 g          837 t
ORIGIN      
        1 aagacaatcc gaagcaaacc gtttgtcagc ctccatatca tcttttaccc ccggaaagtg
       61 atcgcgtttt acaaccagaa tgacagccat actgattatg tcgtctcccg gcccccttca
      121 catataacat gcatgtctgt cattgcttac tgctccacat gaccggattt cacatacagg
      181 atgcacattc agtgttttta tcgccactat aacagacaga ttactgtcgt ctttactctt
      241 cagattgtta atctgaccac aagaacagcc gaacggcaaa acgccctcca caaggggcac
      301 atgaaccata gttgatagca tgtttttact aatcaaggaa tagttcagga atattttatt
      361 gcattaataa ctggcgaata atcgttctgt gaacacaaaa tgcttttatt tgtaaatttt
      421 attgcattgc actcgttttg ttgatatggg gtttaataac agataacccg gaaagaagga
      481 gaaaacagat gacattacgg attaacgatc aggggcgaga taaacagaat caatttttat
      541 cgaaaaatgg agaaaatctg aattcgttaa aatcgcgtca tgtgcaggat aggcatttac
      601 ctgaatggct taaggataaa gttgcagcat tctggttttg tctggtgata aaggatatga
      661 aagtgtcagg gagcagttac tatgaactta ccatgcaact tcccacttct gaagaatctg
      721 tttgcatcag taagctgtca tcctcaaccc atgagattcg gctggagatc atcaggcgat
      781 ggattgatag tgtagcacag atagaaacgg aagcttttac taaaaatctc gttgatgcca
      841 tctataaaaa ctggaaggaa atcgggaaca aaaaccaggc cacatggctt aaaaaaaatc
      901 cggaacaact cgagtgggca tggaaatata ttaacattcg tattcccgag tcacatttaa
      961 cctggttttg ccccgtcaat aacgaagaga aacgcattgc catcttagca ttgcttaaaa
     1021 tactgtatcc cggagatata ccatgccagt acaacgacaa agagtttcat gtcgggatct
     1081 gcgctgaaga acatttttac agaagaatgc acgacgcttt cagaaaacag tttatcgacg
     1141 ggaaaaaaga taaacgggta caaattaacg taaagatatc accgtccgca aaaagcgctc
     1201 tggacagact gaccagagcg aggaaaacaa cacagcaggc tattctggaa caactgattc
     1261 tgaacggtag actggattaa ctttcactac ttccactcct ggcagttttc ctctttttgt
     1321 agtgtctccc ggctgcaggc atcaggtact tttccgtcat ttcgttgcag aactcttcaa
     1381 tgtagttaat cagcttctgg tgtgctttca ccttttcttc cccgggggac gctttcagat
     1441 aaatgtcctc cttttccaca agtgtattta taaactctcc cggacatccc tggttataaa
     1501 gcaacgtgaa aaacaacaca ttccccagtc tctccagtaa ctgcgattta accctgtatt
     1561 gttgtttgct gaggattgat tgttttataa agcttagtct tgttaaaagt tgttcaaatc
     1621 ccggttccag tttaaaaaca gattttaata ccagggactg atactcctga taaaaaagtt
     1681 caaacgtcgg ggatgcagac tgacgaacag atttggctac aacccatgga ctaaatccca
     1741 tgattgggat ttccttcgct ccctccagcg cactcagagg tagtccagca atccatgcgt
     1801 ccagcttagc cgaatcaata tccccgctca aaacctcata caaactgccc gtgaacggtt
     1861 gagtcggttt ttttgttgtt ttaccttctg attgcctgaa tccatccact tttctccgga
     1921 tattacgcgg aatcagcata aaaggcggga cattaagtga taaacgttta cccgtcatac
     1981 gataccagtg gcccacctca accttaatcc gtgtatctgg ccttgtatct ggccttgtat
     2041 ctggccttag ctcaagtgct attatgttaa gcaggtacag ataacgcacc gtataacagt
     2101 caaaatgttt ctccggatca aacggctctg atcgttctct gcacacaagt ccgctgcgct
     2161 tcagtattcg ctgggtatct ttccaggcct tactctgaag aatggtggta actctggctt
     2221 tattagcaat ctcacgcagc gcatcacttt gttgtgccgt catctcttcc ctccacactt
     2281 tgtcactcca gattttttca cgtccatatt acctgaatga taaaccggat tagtgtgatc
     2341 cggttcagcg aaatcaacag gataccggta tgtcattcag ccattcttcc ctctctgcac
     2401 aagtgaaatc atatctgacg tttcttcctg aagaaattcg ccagaaaatc cttgaacatc
     2461 tccacggtgt tattcattac gagcccgtga ttggcattat gggtaaatcc ggcacaggca
     2521 aaagcagcct gtgtaatgcc atttttcagt cccatatctg cgccacgcat cccctgaacg
     2581 gctgcacccg ccaggctcat cacctcaccc tgcagatcgg tgaacgcaga atgacgctgg
     2641 tcgatctgcc cggcattggt gagacaccgc agcatgatca ggaataccgg gcactttatc
     2701 atcagttgct accggaactg gatctgatta tctggatcct gcgggctgat gaacgtgcgt
     2761 atgctgccga tattgccatg catcagtttt tactgaatga agatgccgat ccctcgcgtt
     2821 ttctgtttgt tctcagccat gccgatcgcg tgtttcctgc tgaagaatgg aacgacacag
     2881 aaaaatgccc gtcccgtcag caggaactct cactggcgac agtaacagcc cgggtggcca
     2941 ccctgttccc gtcatcattt ccggtactct ccgttgcagc gcctgtcggc tggaatcttc
     3001 aggcactggt gtcactgatg atccacgc
//
LOCUS       DBBGLQ010000259         2995 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.259, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000259 DBBGLQ010000000
VERSION     DBBGLQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.259"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            102..578
                     /gene="agaB"
                     /locus_tag="ACIYSB_005450"
     CDS             102..578
                     /gene="agaB"
                     /locus_tag="ACIYSB_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGC5432254.1"
                     /translation="MTSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            617..1420
                     /gene="agaC"
                     /locus_tag="ACIYSB_005451"
     CDS             617..1420
                     /gene="agaC"
                     /locus_tag="ACIYSB_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGC5432255.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1410..2201
                     /gene="agaD"
                     /locus_tag="ACIYSB_005452"
     CDS             1410..2201
                     /gene="agaD"
                     /locus_tag="ACIYSB_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGC5432256.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDNTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2202..2957
                     /locus_tag="ACIYSB_005453"
     CDS             2202..2957
                     /locus_tag="ACIYSB_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HGC5432257.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPNAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATDRFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          753 a          695 c          736 g          811 t
ORIGIN      
        1 aaaggagata actaaacctt aataaataca tcactacaat atcgcaacaa taatatattt
       61 aaaaaattta tattattcaa ctttatggtg aggattacac aatgaccagt ccaaatattc
      121 tcttaacccg tattgataac cgtctggttc acggtcaggt tggcgtgacc tggacatcca
      181 ccatcggtgc aaatctgctg gtagtcgtgg atgatgttgt cgctaacgat gatattcaac
      241 agaaattaat gggtattacc gcggaaacct acggctttgg cattcgtttc tttactatcg
      301 aaaaaaccat taacgtcatc ggcaaagctg caccacatca gaagatcttc ctgatttgcc
      361 gtacgccaca aacggtacgt aaattggtag aaggtggtat tgacctgaaa gatgtcaacg
      421 tcggcaatat gcatttctcg gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg
      481 accaggatct cacggactta cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg
      541 acgtccctgg cgatcaaaaa gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg
      601 gtttgaggta ataaaaatgc atgaaataac cctacttcag ggattatccc tggcggcgtt
      661 agtttttgtt ctggggattg atttttggct ggaagcctta tttttattcc gcccgataat
      721 cgtttgtacc ctaactggcg ctattctcgg tgatattcag actggcttaa ttaccggtgg
      781 tctgacagag ttggctttcg ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc
      841 gattatggcg ggtctgatga ccaccgtcat tgcatggtct acgggcgttg atgccaaaac
      901 agcaattggt cttggcctgc cgtttagttt gttaatgcag tacgtcattc tgttcttcta
      961 ttccgctttc tcattattta tgaccaaagc cgataaatgc gcgaaagagg cggatacggc
     1021 agcgttttcc cggcttaact ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat
     1081 tgctttcctc tgtacttacc tggcacaggg ggcgatgcag gcgctggtga aagcgatgcc
     1141 cgcctggctg acccacggct ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg
     1201 cttgctgctg cgcgtaatgt tcaaagcgca atatatccct tacctgatcg ccggtttcct
     1261 gtttgtttgc tacatccagg tcagcaacct gttgccggtt gccgtactgg gcgcaggctt
     1321 tgcggtgtat gagtttttca atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc
     1381 cagtaaaaat gaagaagagg actacagcaa tgggatctga aatcagtaaa aaagatatca
     1441 cccgtctggg ctttcgttcg tcgctgctgc aagcgagctt taactacgaa aggatgcagg
     1501 cgggcggttt tacctgggcg atgttgccga tcctgaaaaa gatttataag gacgacaaac
     1561 cgggcttaag cgcggcgatg aaagataacc tcgaatttat taatacccac ccgaatctgg
     1621 tcggattcct gatggggtta ttaatttcga tggaagaaaa aggagaaaac cgcgacacca
     1681 ttaaaggcct caaagtggca ctgtttggcc caatcgccgg gattggcgat gcgattttct
     1741 ggtttacttt gttgccgatt atggcgggaa tttgctcatc atttgccagc cagggaaacc
     1801 tgctggggcc gattctattt ttcgccgttt acctgcttat ctttttcctg cgcgtcggct
     1861 ggacccacgt cggttattca gtcggcgtga aggcgatcga taaagtgcga gagaactcgc
     1921 agatgattgc ccgttcggca accatcctcg ggatcacggt aatcggcggg ctgatcgctt
     1981 cgtatgtgca tattaacgtg gtgacatcgt ttgccatcga caatacccac agcgttgcgc
     2041 tgcagcagga tttcttcgat aaagtcttcc cgaacatttt accgatggcc tacaccctgc
     2101 tgatgtatta cttcctgcgg gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt
     2161 ttgtgctctc tattgtttgt tccgcattcg gcattttgta aatggaacga ggcactgcgt
     2221 ctggtggtgc ctctttactt aaggaatttc atcctgtgca aacccttcag caagttgaaa
     2281 actatacggc gttaagtgaa cgtgccagcg aatatttatt ggccgtgatc cgtagcaaac
     2341 cgaatgccgt gatttgcctg gcgaccggag ccacgccatt actgacgtat cattatctgg
     2401 tagaaaaaat ccaccagcag caggttgatg tcagccagct caccttcgtg aagctcgacg
     2461 aatgggtgga tctgccatta acgatgccag gcacctgcga aactttcctg caacagcata
     2521 tcgtgcagcc gctggggcta cgtgaagacc agctcatcag ctttcgctcc gaagagataa
     2581 atgagacaga gtgcgaacgg gtaacgaacc tgattgcgcg caaaggcggt ctggatttat
     2641 gcgttctcgg attggggaaa aacggtcatc ttgggctgaa cgaaccggga gaaagcctgc
     2701 aaccggcctg ccatatcagt caacttgatg ccagaacaca gcaacatgag atgttaaaaa
     2761 ccgcgggtcg ccccgtgact cgtgggatca ccttaggcct gaaggatatt ctcaatgccc
     2821 gcgaagtttt gttactggtg actggcgaag gaaagcagga tgcgacagat cgttttctca
     2881 cggctaaagt ctctaccgct atcccggctt catttttatg gctgcacagt aactttattt
     2941 gtttaattaa cacctaacca gatgaaaaat ttgctcaaaa tgtttaaatt aactt
//
LOCUS       DBBGLQ010000260         2977 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.260, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000260 DBBGLQ010000000
VERSION     DBBGLQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.260"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            62..1186
                     /locus_tag="ACIYSB_005454"
     CDS             62..1186
                     /locus_tag="ACIYSB_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5432258.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     MAERYMDEITTVDIATYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIASVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFRDKNQQLYVIFHLALETAMRQGEI
                     LTLRWEHLDLQHGVAHLPETKNGLPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKISPYFVPYPATVRCRNGLFVVTLHDFDLETRAETRELAISH
                     ASVLLLRTLAQAAQRGERVPTPGELPANIDERVMICPLTN"
     gene            1399..2220
                     /locus_tag="ACIYSB_005455"
     CDS             1399..2220
                     /locus_tag="ACIYSB_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HGC5432259.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHAFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PVRMDIEKEGWFQMARKISPDIPVRTVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            2322..>2977
                     /gene="traF"
                     /locus_tag="ACIYSB_005456"
     CDS             2322..>2977
                     /gene="traF"
                     /locus_tag="ACIYSB_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HGC5432260.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTFGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     T"
BASE COUNT          786 a          799 c          688 g          704 t
ORIGIN      
        1 gagacattgc atttctgatt cagtattcct accttcctcg gatttatcgt ggaaggcaga
       61 tatgtttcga aaaataaaaa ttcgcaaaat gactctcaac cgggcgctgg acaaatacct
      121 gaagacagta tccatccaca aaaaaggtca tctgcaggag ttttatcgcg tcaatgtgat
      181 taaacgtcac cctatggccg agcgctatat ggatgaaatc accaccgttg atatcgccac
      241 ttaccgggat cagcgtttgg ctcaaattaa cccacgaaca gggcgtcaaa tcaccggaaa
      301 caccgtccgt cttgaacttg ctcttttatc atcattattc aatatcgcca gtgtggaatg
      361 ggggacatgc cgtatgaatc ctgttgaact ggtccgaaaa cccaaaatca gtagtggacg
      421 cgatcgtcgc ctgacttctg gcgaagaacg ccgactctcc cgctatttca gagataaaaa
      481 tcagcagctc tacgttatct tccatctggc cctggaaaca gcaatgcgac agggggaaat
      541 cctcacatta cgctgggaac acctcgattt gcagcacgga gtggcgcatc ttcctgaaac
      601 gaaaaatggt ttaccacgcg atgtcccact ctcccggaaa gctcgcaact acctgcaaat
      661 tctgccacaa cagataaacg gcaatgtgtt cagttacacc tcatccggtt tcaaaagcgc
      721 ctggcgtacc gcacttctgg acctgaaaat agaaaatctg cattttcacg atctccgtca
      781 tgaagccatc agccgatttt tcgaacttgg tacattgaat gtcattgaag ttgccgctat
      841 ttcaggccac cgctccctga acatgttaaa acggtacacg catctccggg catatcagct
      901 ggtcagcaaa ctcgatgcca ggcgtaaaca gaccagcaaa atttcccctt attttgttcc
      961 ctaccctgca actgtcaggt gcagaaacgg gctgtttgtc gtgacactgc acgatttcga
     1021 ccttgaaacc cgtgcagaaa cccgggaact ggcaatatct catgccagcg tattgttgct
     1081 gcgcacactg gcacaggcgg ctcagcgggg tgaacgcgtt cccacgccag gcgagctacc
     1141 cgcaaacatt gatgaacggg tcatgatttg tccgttaaca aactgaaggc atatcagaat
     1201 gaaaatcaac ttttgcttcc gggttaaatt aatcacggtc atcctgtccg aatccttttc
     1261 attcgccctt aaaacagagt tgcaaacact cttgaaacac actcgatcat aacgatcgaa
     1321 ataactcgct tccgtttaaa aaacactcta tttagactca atttatgaac aaaacacgct
     1381 gacccgcatg gaggtgaaat gaaaactgac agcacaggta ttgccgcccg tatgatgctc
     1441 tccctggaca gggagcgaat ttgcgaatgc ctgctcagtc atcgccagct gcagtccaca
     1501 cccctgcagg ttcgttatcc gcagggagtc agggatgccc ttggtatcat gagcgaacag
     1561 ctttctcttt ccgtctctga cctgacgcgt atcctggtcg aagacgcgtt aagtgaaatg
     1621 tttcttcctg cagataacat cgtccgccgt cttctcagca ggatggagca catcatgcag
     1681 gcacatgata tcagcgcaac aacgatggct gcattactgg ccccctggaa tatccgccct
     1741 gccgtcttcc gggagccgga cagactcact gactatctga ccggggagat tcttgcagca
     1801 ctggctgact ggttttatct cagtccggaa tggctgaacg gcagggtgca ttatccactg
     1861 tatcgtccgg gagactggcc agccacacag gagatattct gcaggattat ttcagcccgc
     1921 gagaatatgg acattattct ctggcatgct tttccgtttg ccggaacgca ttccggagaa
     1981 tattgcggcg ttctgctcag gcagaaaaaa gaaattaata acaccattat ttatccggta
     2041 ctttcgttat atccggtcag aatggatata gaaaaagaag gctggtttca gatggcccga
     2101 aaaatatctc cggatatccc tgttcgcacc gtaacactca ctccggcaca ggcagaatat
     2161 ctgataacag gaaaaatatt acccactgcg ttattcaggg ttccgttatc cccctggtaa
     2221 taatactgag caataaaccg gaataataca ccaactattt ttaatggcac agccgtgccc
     2281 tctgttgtta tacccaaaat acctcaatac aggaaactga aatgatgatg aaaaagcata
     2341 ttacccgtac tctcatcgcg tctgccgttt tattttcctt taattcgact gcagcaacgt
     2401 catattttga agcccgcaat gatgcgatgg gaggaacggg ggttgcctcc tctcactatg
     2461 gtgtggcccc actggcaaac ccggcactgc tgacaaaatc cggtgcaaaa gatgacttca
     2521 gtcttctgct tccgtccgtg ggtgcgcagc tgtccgatcc gggtaatatc gccgacaacg
     2581 ccgacaaaat cagcgatgac tggaaagcct tcgaccgtgc cgtcgacagc cactccgggg
     2641 taccgcagac agcggcccgc ctgaaagaga gactgcagga cttccggaac acccatgcca
     2701 gcgcacaggc cggggtatct gctgtggcgg cccttcccgg tgataccctc gctgctgcac
     2761 tgatgctgaa aacgttcggg actgtcagcg tcgacggtaa agtcagcgat gccgacctga
     2821 actatctgga aagcattgca gacagtggca gccaggatgt ggataaaaac agactgacct
     2881 cacaggcttt tgcccgtgcc gcactgatca ccgatgtggg cgttgcgctg gccactgaac
     2941 tggaaacggc cggacaaaaa tggtcactgg gcttcac
//
LOCUS       DBBGLQ010000261         2903 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.261, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000261 DBBGLQ010000000
VERSION     DBBGLQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.261"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..439
                     /locus_tag="ACIYSB_005457"
     CDS             <1..439
                     /locus_tag="ACIYSB_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HGC5432261.1"
                     /translation="SAVMQNAAVGQATTPAQSTGWPSVTHPALNNAPAVKNTVDLPTW
                     EELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARIIQRTPDE
                     VLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            436..1515
                     /locus_tag="ACIYSB_005458"
     CDS             436..1515
                     /locus_tag="ACIYSB_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGC5432262.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            1515..2063
                     /locus_tag="ACIYSB_005459"
     CDS             1515..2063
                     /locus_tag="ACIYSB_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGC5432263.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNSPKARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            2063..2488
                     /locus_tag="ACIYSB_005460"
     CDS             2063..2488
                     /locus_tag="ACIYSB_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HGC5432264.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            2475..>2903
                     /locus_tag="ACIYSB_005461"
     CDS             2475..>2903
                     /locus_tag="ACIYSB_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGC5432265.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPIACSSAGAV"
BASE COUNT          619 a          745 c          951 g          588 t
ORIGIN      
        1 ttctgcggtg atgcagaatg ccgcagtggg gcaggcaaca acacccgcgc agagcactgg
       61 ctggccttcc gtcacgcatc cggcactgaa caatgcaccg gcggtgaaaa acacggttga
      121 cctgccgacg tgggaagaac tgactgacat tcgcgacaca ctgaatacgg caattgataa
      181 ggagttgtcc cgtacaacca gtgatgcgct gtttctggcg ctgcgccggg tgaaagcaga
      241 tctgaatgcg gatatcaaca cgcgccttga acagtctgca cggatcattc agcgcacgcc
      301 ggatgaggtt ttacccgcgc tggtgctggc ggcgacctgg tttgataacg cggcgcgtga
      361 cgcggacatt atccggcgta atgccattac gcatcccggc tttgtgccgg tgatccctct
      421 gaaggtgcca gtgcaatgaa cgacaatgtc acgctacggg taaatggccg ggagtggaat
      481 ggctggacat cggtgcgcat cggtgccggt attgaacggc tggcgcggga tttcagtgtg
      541 gagatcactc gccagtggcc gggagatgag ggtatcacca cgcttcagcc gcgcattaaa
      601 aacggttcaa aagtggaagt gctgattggt gatgagctgg tgatcaccgg ctgggtggag
      661 gcgactcccg ttcgttacga tgcccgttcg gtcagcaccg gtattgccgg acgtagtctg
      721 acggctgacc tgattgactg tgcagccgaa ccgacacagt ttaacggacg ctcgctggtg
      781 cagattgcgc aggcgcttgc tgcgcctttc ggcattgagg tggtgaacag cggtgcgccg
      841 tcgggtgtta ttcctgatgt tcagcctgat cacggtgaaa cggtgattga ggtaatcaac
      901 aaaatactcg gtcagcagca ggcgctggct tacgacgacc cgcacggcag gctggtgatt
      961 ggcggtattg gctcaacgcg ggcacatacc gcgctggtac ttggggaaaa catcctttcc
     1021 tgtgatacgg agaagagtat ccgggagcgg ttttctgttt accaggtggc ggggcagcgt
     1081 gccggaaacg acgatgattt cggtgaggcc accaccaccg cgctgcgggc ccgcacagag
     1141 gacgcattta ttgcccgtta ccgtccgatg tatatcaggc agacagggca ggccacgggg
     1201 gcaggctgta ttgcccgtgc ggactttgaa gcccgacaac gggcggcgcg gacggatgaa
     1261 accacctatg tggtgcaggg ctggcgacag ggtaacggta cgctgtggca gccaaaccag
     1321 cgggtgattg tctttgatcc ggtctgtggt ttcgacaata ccgaactgct tgtttcggaa
     1381 gtcacgttta ctcaggacca gaacggcacc ctgacggaaa tccgtgtcgg cccacctgat
     1441 gcttatctgc ctgagcccga agcccccggc gcgcggaaaa agaaaaaagc cagagtacag
     1501 gaggacccgt tctgatgagg acgattgaag ccatgcagcg acaactcctc ggcctgattg
     1561 ggcgggccgt tgtgaaaagc atcagtgccg ccacgaaatg tcagaccgtg gatgtgtccc
     1621 tgattgccgg tgaacccaaa gcaggggttg aacatcttga accctacggt tttaccgcaa
     1681 gggcaaacag cggtgcggaa gcggtggtgt tgtttccgga tggcgaccgt tctcatgcgg
     1741 tggttgttac ggtgtcggac cggcgctacc gcctgaaagg gctgcagacg ggtgaggtgg
     1801 ctgtctatga cgatcagggg cagtccgtga cgctgacccg ggaggggatc gtggtggacg
     1861 gtgcaggtaa aacgatcacg tttcgcaatt cacctaaagc acgttttgaa atggacctgg
     1921 aagtgacagg acaggtgaaa gacctgtgcg actccggcgg caccaccatg tcagcgatgc
     1981 ggcttgccta taacgggcat cgtcacagag agaacggtca gggaagtaat accgacaaac
     2041 ctgataaagc gatggaggca tgatggaact gtggctgacg gtgaacggta aacgcacctg
     2101 cgccagcgca ccgctggatc cgctgacccg cgccgtggtg atttccctgt ttacctggcg
     2161 gcgggcggag cctgatgaca acgccgacgt cccgatggga tggtgggggg atacctggcc
     2221 tgcggtacag aatgaccgtt acggctcccg actgtggctg cttcagcgca gcaaactgac
     2281 caatcagctg gtgcagacgg taagggggta tatccgcgag tgcctgcaat ggatgattga
     2341 tgatggcgtg gtgtcccgta ttgatctgga tatccgccgc accgggatta atgaactggg
     2401 taacagtatc actctctggc gtcgtgacgg accggtaatg atttcttttg atgatctgtg
     2461 gagtgcgata acgcatggcg gacagtgaat ttcagcgccc gacgctggca gaaaatatca
     2521 gtatgctccg taacgattta ttcgccaggc tggacgtcag cgacacgctc cggcgcatgg
     2581 atgaagacgt gcgggcaaag gtgtatgcgg cggcgctgca tacggtttac gggtacatcg
     2641 attatctggc aatgaacatg ctgcctgacc tgtgcgatga gtcctggctg gcgcgacatg
     2701 ctgcgatgaa acggtgtccg cgcaaggggg ccacggctgc cagcgggtat atgcgctggg
     2761 aaggtgtcag cgatggcctg aaggtgaccg ccgggagtgt tattcagcgc gatgacctgg
     2821 ttcagtacac ggcaactgcc gatgcaacca gctccggtgg tgtcctgcgc gtgccgatcg
     2881 cctgctcaag tgcaggcgcg gtc
//
LOCUS       DBBGLQ010000262         2880 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.262, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000262 DBBGLQ010000000
VERSION     DBBGLQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.262"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(60..1250)
                     /gene="aspC"
                     /locus_tag="ACIYSB_005462"
     CDS             complement(60..1250)
                     /gene="aspC"
                     /locus_tag="ACIYSB_005462"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGC5432266.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1435..2523)
                     /gene="ompF"
                     /locus_tag="ACIYSB_005463"
     CDS             complement(1435..2523)
                     /gene="ompF"
                     /locus_tag="ACIYSB_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGC5432267.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADIGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEDIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          739 a          683 c          671 g          787 t
ORIGIN      
        1 atgttgcgtt tgtcatcagt ctcagcccgc ttttcagcgg gcttcattgt ttttaatgct
       61 tacagcactg ccacaatcgc ttcgcacagc ggagccatgt tatctggtgt catcccggcc
      121 acatttacgc gaccagaagc aaccgcatat acgccaaact cttcgcgcag acgcagcact
      181 tgttcttttg tcaggccact gaaggagaac atgccgttct gtttgatgat aaagctgaag
      241 tcgcggtttg cgcctttttc ctgcagcgta ttgacgaaca actgacgcat acgctgaata
      301 cgctggcgca tatcagtcag ctcttgttcc caaatcgcac gtaacgcatc gttgctcagg
      361 atggtggcaa caacagaagc gccgtgtgct ggtgggttag agtagttagc gcgaatcgcc
      421 gctttcattt ggctgaatgc gcgatcaacg gtttcactgt cggcagcaac cagagtacaa
      481 gcgccaacac gctcgttgta caggccaaag tttttagagt aggaactggc aacaatcagc
      541 tctttatgca tagccgcgaa agcgcgcagt ccttcagcat cttcttccag accacgggca
      601 aaaccctggt aagcgaagtc aaacagcggt aaccagcctt tctcaacgga gagttgtgcc
      661 agtgtttgcc attgttccag cgtagggtcg ataccggttg ggttatggca gcagccatgg
      721 aacagcacta cgtcgccagc ctgagcttca ttcaggctgt taatcagtgc atcgaagtca
      781 agagtgtgat tttccgcatc ataataagcg tattcacgaa cttccagacc tgcagagtta
      841 aagacgctct tatggttcgg ccagcttggg ttgctcaccc acacacgctt aacgctggta
      901 ttttttgcca ggaaatcggc agccacgcgt agtgcgccag tgccccccgg agtctgtgcc
      961 gtgcgagcac gtttgtcatt gatcagggcg ctacctttac caaacagcag ttcctgagtg
     1021 cagcgaccaa attcagggat gccgtcaatg ccgaggtaat ttttggtggt ttcattttcg
     1081 agcagatact gttcagcctt tttcacgctg gtcagtaccg gggttttgcc cgtctcatct
     1141 ttatagacac caatcccgag gttaattttg ccgggacgtt catcggcacg aaacagatcg
     1201 gccaggccca gaatcgggtc ggcaggagcg gcggtaatgt tctcaaacat gacgaggttc
     1261 cattatggtt acagaaggga agtccgctat cagggtaacg ggagatttac aaaattccaa
     1321 ctattactga tgaaaactca ggctgttttt gcaagacgtg agattgctct ggaaggtata
     1381 aaaaaaacag gaccaaagtc ctgttttttc ggcatttaac aaagaggtgt gctattagaa
     1441 ctggtaaacg atacccacag caacggtgtc gtctgaacct acgcccagtt tgttgtcaga
     1501 atcgatctgg ttgatgatgt agtcaacata ggtggacatg tttttgttga agtagtaggt
     1561 tgcgcccact tcaaagtagt tcaccagatc aacatcaccg atatcttcta cgtctttcgc
     1621 tttagatttg gtgtaagcga tggacggacg cagaccgaaa tcgaactggt attgcgcaac
     1681 taacagaacg tcttgcgttt tgttggcgaa gccgctggtg tttgtaaatt tattagtgat
     1741 cggcgtagcg ttacgggttt caccgtagtt cgctgccagg tagatgttgt tcgcgtcgta
     1801 cttcagacca gtagcccact gttcagcttt tttaccgttg ccaagaggtt gagcttcttg
     1861 caggttggta cggtcagctg caccataagc accaacgata ccaaagcctt cgtattcgta
     1921 gctgatagaa ccgccaacac cgtcgccgtt agagcggcgt gcagtgtcac gctcgttttt
     1981 acccaggtac tgaacagcga agttcaggcc atcaaccaga ccaaagaagt tggagttacg
     2041 ataggtagca acgccgccaa cacgaccaac gaagaagtca tcgctgtatg cagtatcacc
     2101 accaaattct ggcagcatat cggtgtaacc cagtgcatca taaaccacac cgtagttacg
     2161 gccgtaatcg aaagaaccaa tgtcagcgta tttaagaccc gcgaatgcca gacgcgtttt
     2221 gttaccagtt tgagcgtcag cgccttcaga gttgttaccc tggaagttat attcccactg
     2281 accataaccg gtcagatcgg aattgatttg tgtttcccct ttaaaaccaa gacgggcata
     2341 agttttgtcg ccgtttccgc cgtaactgtt tacaccattg tctttagaaa aataatgcag
     2401 accgacagct ttaccgtaca gatctacttt gttgccatct ttgttataga tttctgcagc
     2461 gtttgcagta cctgctacta acagagcagg gacgatcact gccagaatat tgcgcttcat
     2521 cattatttat taccctcatg gtttttttta tgacacctgc cactgccgtc aataagttct
     2581 gtcaataaaa atttacggaa ctattgatga gagtttggtg tctttatgtg tctgcaggca
     2641 tctttccatt caaactaacg tttcgctacc gtgaaagtgc tacaaagata aagatttggt
     2701 ttcaaaaaga aaaaatatgt aaccaaaagt aaaatttaag gaactttgtg aacaccgtca
     2761 tatttccata gaagcgtgat gatatttaca gcaattttta tctatttata tgatttcctt
     2821 atatttaaat taactaaacg gaaattttgt ttctgatgga aactttatcg acctggcact
//
LOCUS       DBBGLQ010000263         2832 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.263, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000263 DBBGLQ010000000
VERSION     DBBGLQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.263"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..656
                     /locus_tag="ACIYSB_005464"
     CDS             <1..656
                     /locus_tag="ACIYSB_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HGC5432268.1"
                     /translation="EGTTEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYY
                     NRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPK
                     SPAQQQADDNAAKALTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITD
                     RVNDIINDRFWSDGKKPDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            677..1519
                     /gene="yibA"
                     /locus_tag="ACIYSB_005465"
     CDS             677..1519
                     /gene="yibA"
                     /locus_tag="ACIYSB_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGC5432269.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1547..2505
                     /locus_tag="ACIYSB_005466"
                     /pseudo
     CDS             1547..2505
                     /locus_tag="ACIYSB_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2517..>2832
                     /locus_tag="ACIYSB_005467"
     CDS             2517..>2832
                     /locus_tag="ACIYSB_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGC5432270.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIYKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNP"
BASE COUNT          913 a          522 c          655 g          742 t
ORIGIN      
        1 aggaagggac aacagaatgg tgcgcagaat acgatgaatg gggcaacctg ctgaatgaag
       61 agaacccgca tcagctgcag cagcttatcc gcctgccggg gcagcagtat gatgaggagt
      121 ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcgg tatatcactc
      181 aggatccgat tgggctgaag gggggatgga atttttatca gtatccgttg aatccagtta
      241 cgaatacaga tcctctgggg ttagaagttt ttcctagacc attccccttg ccaattccat
      301 ggcccaaaag ccctgcacag cagcaagcag atgataatgc tgcaaaagca ttgacaaaat
      361 ggtggaacga tacagcatca caaagaatat ttgactctct aatattgaat aatccgggac
      421 tagcattaga tataacaatg atagcttctc gtggaaatgt tgcagacaca gggataactg
      481 atcgtgtcaa tgacataata aatgacagat tctggagtga tgggaaaaaa cccgacagat
      541 gtgacgtact tcaggaacta attgattgtg gtgatattag tgctaaagat gcaaaaagca
      601 cacagaaagc ctggaattgt cgtcactcca gacagtcaaa cgataaaaaa agatagccct
      661 tgtggaggtt cctgcaatgt caaatacata ccagaaaaga aaggcaagta aagagtatgg
      721 tttatataat caatgtaaga aactaaatga tgatgaatta tttcgcttac ttgatgatca
      781 caattccttg aaaaggattt catctgccag agtattacag ttaagaggtg ggcaagacgc
      841 tgttagattg gcaattgagt tctgctctga taaaaattat atccgtagag atatcggagc
      901 atttatactc gggcaaataa aaatttgcaa aaaatgcgaa gataatgttt ttaatatttt
      961 gaacaatatg gcattgaatg ataagagcgc ttgcgttcga gctacggcaa tcgagtcaac
     1021 ggctcagcga tgcaagaaaa acccaattta ttcacctaaa atagtagaac aatctcaaat
     1081 tactgctttt gataaatcga ctaatgtcag acgtgctaca gcatttgcta tttctgttat
     1141 caatgataaa gcaacaattc cactattgat taatctgtta aaagatccaa atggagatgt
     1201 cagaaactgg gccgcatttg caataaatat caataaatat gataatagtg atattaggga
     1261 ttgttttgtg gagatgcttc aggataaaaa tgaggaagtc cgtattgaag caataatcgg
     1321 actttcctac agaaaagata aaagggtttt atctgtttta tgcgatgagt taaaaaaaaa
     1381 tactgtttat gatgatatca ttgaagctgc gggtgaatta ggtgataaaa cgctacttcc
     1441 tgttttagat actatgttgt acaagtttga tgacaatgaa attataactt ccgctattga
     1501 taagctgaag cgttcatagc gtgagttgcc tatgcacagt gggggattcc cgccggcacg
     1561 gtgcagatgc tcgaccggct ggaaagtgaa atcctggctg accgggtgaa tgaggaaagc
     1621 cgccgctggc tggcatcgtg cggcctgacc gtggagcaga tgaaaaacca gatggacccg
     1681 gtgtacacgc cggcgcgaaa aatccacctg taccactgcg accatcgcgg cctgccgctg
     1741 gcgctcatca gcacggaagg ggcaacagcg tggtgcgcag aatatgatga atggggcaac
     1801 ctgctgagtg atgagaaccc gcatcatctg cagcagctca ttcgcctgcc gggtcagcag
     1861 tatgatgagg agtccggcct gtattacaac cgccaccgct attatgaccc gctgctgggg
     1921 cggtatatca ctcaggatcc gattggactg aaggggggat ggaattttta tcagtatccg
     1981 ttgaatccgg tcataaatgt agatccgcaa ggtttggttg atataaattt ataccccgaa
     2041 agtgatctta tccattctgt agctgatgag attaatatcc caggcgtttt cacaatcggg
     2101 gggcatggta cccccacatc tattgaatcc gcaacgcgca gtatcatgac agctaaagat
     2161 ctagcatatc taattaaatt tgatgggaat tataaagatg ggatgacagt ttggttattt
     2221 tcttgtaata caggtaaagg acaaaattca tttgctagcc aattagctaa agagttacat
     2281 acaaatgtaa taggacctga cacgctatgg acgtggtggg ggcgaggaac taatggtaag
     2341 ttaaaaatgg atacagtgct aacagcacca acgaacctta attcaaataa ggatctaatg
     2401 gctataacaa caaaagacct tggtaattgg ataacatatg ggccatctgg gcaccccatt
     2461 tctaatatgc aaggtacgcc agaaaaaccc agtgatataa gataggttgt agatgtatga
     2521 aagcatgctt gttactattt ttttatttct cttttatttg tcaattgcat ggtgctgatg
     2581 tgaaaataaa acaaaacgaa agtatgatgg ggtctacagc aatgacctat gatttaagtg
     2641 aagaaaagtt gatgaaactg aagtataaat cacaacatgg tgattcagag gcatcatttc
     2701 gactatatca atattactgc tttactaaaa ataacattta caaacaattg cgattcttgg
     2761 aaagatcagc atctcagggg aatgttacag cgcagtttaa ttatggggtc tttttatcag
     2821 atacaaatcc aa
//
LOCUS       DBBGLQ010000264         2826 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.264, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000264 DBBGLQ010000000
VERSION     DBBGLQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.264"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(1..488)
                     /locus_tag="ACIYSB_005468"
                     /pseudo
     CDS             complement(1..488)
                     /locus_tag="ACIYSB_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            610..807
                     /locus_tag="ACIYSB_005469"
                     /pseudo
     CDS             610..807
                     /locus_tag="ACIYSB_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
     gene            1270..2436
                     /gene="sopA"
                     /locus_tag="ACIYSB_005470"
     CDS             1270..2436
                     /gene="sopA"
                     /locus_tag="ACIYSB_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HGC5432271.1"
                     /translation="MRLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIK"
     gene            2436..>2826
                     /locus_tag="ACIYSB_005471"
     CDS             2436..>2826
                     /locus_tag="ACIYSB_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGC5432272.1"
                     /translation="MKRAPVIPKHTLKTQPLEDTSSSAPAAPMVDSLIARVGAMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGIIEIADGSRRRKAAALTE"
BASE COUNT          702 a          627 c          741 g          756 t
ORIGIN      
        1 gactcatagg tatgttcagc atcctgcttt ggtgccacga agacatggac gcgacgtgag
       61 aaccacagtg tattaaccgc gaagttaacc ctgcatcgtt gcccggtaac ctcagcctca
      121 aattctcccc agtcatgctg tagctggtaa ccgggcttga gtttcgaagt gaaccgtttt
      181 cttcgacggc tgcatcttac gtttgggctg gatgtagtaa cgcaacatgg aacgaccacc
      241 gatttaaccc atcgctttga tttctaccag gaggacctcg ccgttccaga cgttctcagc
      301 ctgacgcatg tcgatatagc ccatgaacag cttgagtttg gccattttgt ggcgcgtttt
      361 tctggctgga ggttcaggta tcgtatgaca gtccgctcag agcaaacaac ctgagtggca
      421 atatcaacaa tatacgcacc ttgctggcgc atttgctttg tcatgtaaaa gtcctctctg
      481 ctcagcatgc tgatgtcctt tctggtgtga gaacctcaag gaaacaatat gttgggtgga
      541 gcggaccatc caaatggtga attactgtct tatatcactg gcgctgacac gacggattat
      601 ggtccccagg ccagtgcacg tctgttatca gataaggtct cccgtgaact ttacccggtg
      661 gtgcatgtcg ggggggaaag ctggcgcatg atgactaccg atatggccag tgtgccggtc
      721 tccgttatag gagaagaagt agctgatttc agccaccggg aaaatgacat caaaaacgct
      781 attaatctga cgttctgggg aatataaatt agacatcctc cacgtttttc agggtgtgta
      841 ggatgtgtgc gcccagcatg ggcgcgatct tggaggtgaa agccctcccg cgagctgact
      901 acagcgagcg aaaggaagtg caactgtgtg agggtgaccg agcgtgggaa ggaagcacgg
      961 aatgaccccg tgaaccgatg aataagaatc tgatatgagg tgcgatggaa caaggcgagc
     1021 tggctttagg cttcccttga ctcgcataac ctcaacttct cgaactgccc ggttggagct
     1081 gcatgctgag tggtgtagca gggcgcagtc ttgtgctacc ccctatgcca atcgataagg
     1141 tgtttgttct tcttatattt tttgttttgt tgctcttatt ttaaacaact ttgcaatttt
     1201 attgttgctt tgcaattaaa tgcaagattt aataaaaaaa tgcaaagcaa tgattaaagg
     1261 atgttcagaa tgaggcttat ggaaacactt aaccagtgca taaacgctgg tcatgaaatg
     1321 acgaaggcta tcgccattgc acagtttaat gatgacagtc cggaagcaag gaaaatcacc
     1381 cgacgctgga gaataggtga agcagcggat ttagttggag tatcttctca ggctatcagg
     1441 gatgccgaga aagcaggccg gctaccgcac ccggatatgg aaatacgagg gcgggttgag
     1501 caacgtgttg gttatacaat tgaacaaatt aatcatatgc gcgacgtgtt tggtacgaga
     1561 ctgcgacgtg ctgaagacgt atttccgccg gtgattggag ttgctgctca taaagggggc
     1621 gtttacaaaa cctctgtttc tgttcatctt gctcaggatc tggctctgaa gggactacgt
     1681 gttctgctcg tggaaggtaa cgacccccag ggaacagcat cgatgtatca cggctgggtg
     1741 ccagatcttc atattcatgc agaggatact ctccttcctt tctatcttgg ggaaaaggac
     1801 gatgtcactt atgcaataaa gcctacttgc tggcctgggc ttgacattat tccttcctgt
     1861 ttggctctgc accgcattga aactgagcta atgggcaaat ttgatgaagg taaattgccc
     1921 accgatccac acctgatgct ccggctggcc attgaaaccg tcgctcatga ctatgatgtc
     1981 attgtcattg acagcgcgcc taacctgggt atcggcacga ttaatgtcgt atgtgctgct
     2041 gatgtgttga ttgtccccac gcctgctgag ttgttcgact acacttccgc tctgcagttt
     2101 ttcgatatgc ttcgtgatct gctcaagaac gtagatctta aagggttcga gcctgatgta
     2161 cgtattttgc ttaccaaata cagtaatagt aatggttctc agtccccgtg gatggaggag
     2221 caaattcggg acgcctgggg aagcatggtt ctaaaaaatg ttgtacgtga aacggatgaa
     2281 gttggtaaag gtcagattcg gatgagaact gtttttgaac aggctattga tcaacgctct
     2341 tcaacaggtg cctggagaaa tgccctttct atctgggaac ctgtctgcaa tgaaattttc
     2401 gatcgtttga ttaaaccacg ctgggagatt aaataatgaa gcgtgcgcct gtaattccaa
     2461 aacatacgct taagactcaa ccgcttgaag atacttcgtc atcggcacca gctgcaccga
     2521 tggtggattc gttaattgcg cgcgtaggag ctatggctcg tggcaatgct attactttgc
     2581 ctgtatgtgg tcgggacgtg aaatttaccc ttgaggtgct ccggggagat agtgttgaga
     2641 agacctctcg ggtatggtca ggtaatgaac gtgaccagga actgctaact gaagatgccc
     2701 tggatgatct tatcccttct tttctactga caggtcagca gacaccagcg tttggtcgaa
     2761 gagtgtctgg gatcatagaa attgccgatg ggagccgccg ccgtaaagct gctgcactta
     2821 cagaaa
//
LOCUS       DBBGLQ010000265         2815 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.265, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000265 DBBGLQ010000000
VERSION     DBBGLQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.265"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            381..1013
                     /gene="yqeH"
                     /locus_tag="ACIYSB_005472"
     CDS             381..1013
                     /gene="yqeH"
                     /locus_tag="ACIYSB_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5432273.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1347..2156
                     /gene="yqeI"
                     /locus_tag="ACIYSB_005473"
     CDS             1347..2156
                     /gene="yqeI"
                     /locus_tag="ACIYSB_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5432274.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVANCLTLSYREVNRD"
     gene            2149..2631
                     /gene="yqeJ"
                     /locus_tag="ACIYSB_005474"
     CDS             2149..2631
                     /gene="yqeJ"
                     /locus_tag="ACIYSB_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HGC5432275.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(2664..2744)
                     /locus_tag="ACIYSB_005475"
     CDS             complement(2664..2744)
                     /locus_tag="ACIYSB_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432276.1"
                     /translation="MKDVDQIFDALDCHILREYLILLFYD"
BASE COUNT          984 a          494 c          492 g          845 t
ORIGIN      
        1 cctatcactt ctctcgtcat atcctagtat tatcagttag ttaaacacac aaagagatct
       61 gaacatctta aataagacta tttaagatgc ataacttaga ttcgcaagat atcttctggt
      121 cattcaaaaa caatttgcga tatagatatt cttataccat gagatagtta tctggaccgc
      181 tctcaaccat gcaagattaa atcagtgaaa cataatcata tttgataacg agaaatgcat
      241 ttttaaatgc cttactcaga attatagcaa atacagatta atcgcaattt ttcccggcaa
      301 aattacaaaa tcatggtgta ttgacatcat caataaccaa tgaaattatg caattatata
      361 cggataggga ggttcttaac atggggcttt gtagtcgtta taaaagtctt acatgcaata
      421 gttgctcaat gcattgccaa ataatgccag aagagtcacc gcgtttgcag tattgcgcaa
      481 actcgtgttt ttgtatgtgg cccgaagaaa gttcatattt taatcgtggt gtggtagaag
      541 gtatcttaac aaaaaaccac aatgcgagat taagcggata tatttttgtc gatttttcag
      601 taagtttttt acgtctattc cttgaaaaag actggattga ctatcttgca agtactgaca
      661 tggggattgt tttagtcagc gaccgcaata tgcaatcgtt agctaattac tggcgaaaac
      721 acaactcagc aatatctgct gtcatatata atgatgatgg tcttgatgtt gccaatgaaa
      781 agatcagaca actatttatt ggtcgttatt tatcatttac cagaggtaac acattaactc
      841 agatggaatt caccataatg gggtatatgg tttctggtta taatccatat cagattgctg
      901 aagttctgga tatggatatc cgtagcatct acgcgtacaa gcaacgaatc gaaaagagaa
      961 tgggtggtaa aataaacgaa ttatttattc gttcacattc ggtccaacat tgatactcaa
     1021 cgaccagcca gaatcatact ctgtttatac gggcatttat tgaaatgtcc gtattttata
     1081 aataggccgt cccctcaaga gtaaacacca tagtatttaa aaccatcaca cgaaaaattc
     1141 agaagcatta cgaaatatga atttacaaac aatagtggca taaatgttaa ccatgttaat
     1201 ttacgtaaag ttttacgttg caacattaaa gcctcatttc aatcatcatg ataaatataa
     1261 aattaatata tatttatgcc gtaaatacca atatacttag caaactatgt gatctccatt
     1321 tcgattgatt tagtgtttat tgacgtatgt actggattat taacgataat atcgagttct
     1381 ggcctgagca ccgaaaatta atatcggtac ataacgccga tcttaacgtc gttctgacaa
     1441 cgccagccag tcgatgttta tcacttctac ttgaagcttt tcctgatgtg gttgcacaac
     1501 aagatttttt caccagagtc tgggaagaag aaggtatgcg tgtgcctact aacacgttat
     1561 atcagaacat atccattatc agacgcggat ttcgcgctgt tggtgatact acccactcgc
     1621 taattgcaac cgtgccgaga agaggattca agatccataa tgacattaac atacaaaatc
     1681 atgtaataaa ctcgtcaaca gacgcacata cacacaatgc cccacctgcc ataaaagtta
     1741 atgcgggata caaagagagc attggtggcg caaagaattt caataacaaa atcctcaaac
     1801 atataaaaag tcatctaatt atgttgagcg catttgtcat aggcgcatat tctgcatatt
     1861 ggctatggaa taataatcaa ccgaagccat ttttcaagga ttacaagact gttgcggaaa
     1921 ttaatggctg ccattttaat gtaacagaag atacaattga tgggttgaaa gagttcgata
     1981 aatataaaac acggatactg gattcgggga ttaactgtaa aaaacatccg tggttatact
     2041 tccctcttgc aaaatcctca ccggggatga ttgttatggc atgcaataaa aactataacc
     2101 aacatgaagt ggcaaactgt ttaaccctgt cttaccgtga ggttaatcgt gattgattat
     2161 aaaaaaaact tattatttat tttagttttt atcagtgggt ttattctctt cactgtctat
     2221 tcttacactg cagaaaaaat gatatataac gaaacatgca ctgcaaattg ggtaattttt
     2281 aatgaccagg gacgagcaaa tcttaccatc gactttatgt ataacaaaaa aaataaaaca
     2341 ggaactgttg cgttgagtgg aacctggcaa caaggtaaca gagaaagtaa atcaatacgg
     2401 cgaaatattg aatacacatg gattgaaaac tatgacacag cccatttaac atcaaagaaa
     2461 gttaataagt ttgagattat ggatcaagtt gatgatgata gacttgcaca acttattcct
     2521 gatttttatg tctttccaga aaaaagtgta agctataata tactaaagca aggtaagcat
     2581 gcttttattt tgagcattgg taacagagca ataatgcatt gtgcaaggta aactaaaata
     2641 gataacaaaa agatggaacc tcgttaatca taaaacaata aaattaaata ttctcgcagt
     2701 atatggcagt ctaaagcatc aaagatttga tcaacatctt tcattttaga catctccttg
     2761 caatgtaaaa tatcatgtat caacaacatc tggtgcaaat ccatgagtcg aactc
//
LOCUS       DBBGLQ010000266         2763 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.266, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000266 DBBGLQ010000000
VERSION     DBBGLQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.266"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            2..2763
                     /locus_tag="ACIYSB_005476"
                     /pseudo
     CDS             2..2763
                     /locus_tag="ACIYSB_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT          585 a          811 c          907 g          460 t
ORIGIN      
        1 aatgaagcga catctgaaca ccagttaccg gctggtatgg aatcacgtca cgggcacctt
       61 cgcggtggcc tctgaactgg cccgcacgcg ggggaagcgt gcgggcgtgg ctgccgcact
      121 gtcccttgct gcagtcatgt cagcgcccgc gccggctgct gacagcgctg tacaggcagg
      181 agatacagtg aatggcggta cgctgacaaa tcacgacaac cagattgtct tcggtacggc
      241 cagtggcatg accattagta ccgggctgga gctggggccg gacagtgagg aaaacaacgg
      301 tgggcagtgg atagataatg gcgggacagc cagcaacacc acggtcaccg caaatggtcg
      361 tcaggttgtg atgacgggcg gtacagccac tgacaccgtt atccatgccg gcggggggca
      421 gagcctccgg gggcagtcgg tgaacaccac cctgaacggt ggtgagcagt gggtacatca
      481 gggaggcagg gctgtcggta ctgtcattaa ccgcgacggt tatcagaccg ttaaacccgg
      541 cgcagtggca accgacaccg ttgtgaatac cggcgctgaa ggcgggccgg actctgataa
      601 cgatgccacc ggtcagctcg tggcagggat ggcactctcc accaccgtga acagaaacgg
      661 gcggcaggtc attgcgggcg aaggtatggc aggagaaacc accatacagg ccggtggcga
      721 ccagtcggta catggtcacg ctctgtccac cacccttaac ggcggtaacc agtatgttca
      781 tcagggcggt gtggcacaga acacggtgat taacgacggt ggctggcagg tggtgaagac
      841 gggagcggtg gctgagaata ccaccatcaa ttagaaagga aagctgcagg tgaatgccgg
      901 tggtaaggcc accgccgtca cccagaatac gggcggtgcg ctggttaccg gtacggcagc
      961 caccgtctcc ggcacaaacc gtgcgggcca cttttccgtg gcagacggaa aggctgacgg
     1021 cgtgctgctg gagtcaggcg gtcgtcttga tgtactgaac ggtcacacgg ccactgacac
     1081 ccgggtggat gacgggggca cgctggcagt cctgaccggt ggtacagcca ccacggtatc
     1141 catgggcaga ggcggtatgc tgctggccga ttccggtgcc actgtcagcg gtcagtatga
     1201 cggtggaggt gccttcagta tcggcagcgg acacgcctca ggactcgcgc tgggcaaggg
     1261 aagcgccttt accctgaagg ccggcgacag cgcccgtgac accaccgtga acggcgggca
     1321 gctcaccgcg cagggcggaa ccctgaccgg caccaccacg ctgaataacg gtgccacgct
     1381 gaccctctcc gggaagacgg tgaatgacgg caccctggtc acccgtgaag gcgatgcgct
     1441 tctgcagggg ggcgtactca ccggtaacgg cagggtggaa aaatcaggaa gcggcacact
     1501 cactgtcagc aacaccactc tcacccggaa cgccgtcagc cttaacgaag gcgcactgac
     1561 gctggccgac agtaccgtca ccacggatat cattgcccgg cgcggtacca ccctgagcct
     1621 gaccggcagc accgtgctga ccggtgccat tgaccccacg gatgtcaccc tcacctccgg
     1681 tgccacctgg aacattccgg acaacgccac ggtgaaatcg gtggtggatg aactccgtca
     1741 tgccgggaag attaacttcg tcagcacccg cagcggagcc ttcacgccgg ccaccctgac
     1801 ggtgaaaaat ctccggggac agaacggcag catcaccctg cgtgtccgcc ctgacctggc
     1861 acagcacaat gccgacaggc tggtgattga cggtggcagt gccaccggga aaaccaccct
     1921 gaacctggtg aacgccggca acagtgcgtc ggggctggcg accaccggta agggcattca
     1981 ggtggtggag gccattaatg gtgccaccac ggaggaaggc gcttttgttc agggcaaccc
     2041 gctgcaggcc ggggtgtatg actacaccct gacccgtgac ggtgaccaga gctggtatct
     2101 gcgcagtgaa acgcgttatt gcgcggaagt ccccctgtac gcctccatgc tgacgcaggg
     2161 gatggactac gaccggattc tggcaggctc ccgcagccat cagagcgatg tgaacggtga
     2221 aaataacagc gtccgcctca gcattcaggg cggtcatctc ggtcacggta acaacggcgg
     2281 tattgtccgt ggggccacgc cggaaagcag cggcagctat ggcttcgtcc gtctggaggg
     2341 ggacctgctg agaacagagg ttgccggtat gtctgtgacc gcaggggtat atggtgctgc
     2401 gggtcattct gccgttgatg tcagggatga taacggctcc cgcgccggca cggtccggga
     2461 tgatgccggc agcctgggcg gatacctgaa tctggtacac acgtcctccg gcctgtgggc
     2521 tgaccttgtg gcacaggaaa cccgccacag catgaaagcg tcatcggaca gtaacgactt
     2581 ccgcgcccgg ggccggggct ggctgggctc actggaaacc ggtctgccct tcagtatcac
     2641 tgaccatctg atgctggagc cacaactgca gtacacctgg cagggactct ccctggatga
     2701 cggccaggat aacgccggtt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc
     2761 cgg
//
LOCUS       DBBGLQ010000267         2736 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.267, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000267 DBBGLQ010000000
VERSION     DBBGLQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.267"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(1..585)
                     /locus_tag="ACIYSB_005477"
                     /pseudo
     CDS             complement(1..585)
                     /locus_tag="ACIYSB_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671526.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            649..1120
                     /locus_tag="ACIYSB_005478"
                     /pseudo
     CDS             649..1120
                     /locus_tag="ACIYSB_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028172.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1178..2058)
                     /locus_tag="ACIYSB_005479"
                     /pseudo
     CDS             complement(1178..2058)
                     /locus_tag="ACIYSB_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317439.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2149..2736
                     /locus_tag="ACIYSB_005480"
                     /pseudo
     CDS             2149..2736
                     /locus_tag="ACIYSB_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          696 a          653 c          629 g          758 t
ORIGIN      
        1 acttcattca gcgttctgaa cagataaaaa tcgagtattt ctgtacggtg tgtcctgtta
       61 aaacgcccaa taaaagcgtt ctacgtcagc ttacccggct ggataaactc ccgttttact
      121 gcgtgtatat cagcccattc agccagcgtt agagagataa attccgaacc attatctatg
      181 cgcagcatga ccggataact gcgatttgcc gcgaacctgc tgcctcagaa cctgaaaaag
      241 ttttgaaaaa ccgtatcgag gatatcgctc gtccactgcc tgcaccacca caataacggg
      301 gtcgtaccac gtggtatccg gacggtaatg ggaaaccgtt ctgctctggt tcagactccg
      361 gcaggcctga tggacactga gtccaaatgt cgttatctga tgagtaacaa gctcacgctt
      421 acaggctggt tttaaagctt ttttcgataa tgtctttcaa tgccggttct ctaggctaag
      481 gtcggcaagc atctgttttg gacgtcggtt ctcgtcctca agatcctcgt tctttttaat
      541 atcaggtgac tctatgcatc gtatctgggc ttccagttca tggaatggag taccccctcc
      601 ttctgcggtt aactgtacga aatgcactca ctcagcaggg gaatcacaat gaatatcgta
      661 ttcctgggtg ttgatctgga taaaaatatc tttcagctct gcgggttaaa ttaggtcggt
      721 aatccggttt attcaaaacg tattggccga aaagaattac tccaaatagt ggcgaatatt
      781 ccggccttcc taattgatgt agggcatcca catgggaatt ttactggcag agtgaattta
      841 aaaaactggg gcataacgta aaggtcctca acccacagta tgtaactccc tttgtctgtg
      901 ggcaaaaaat gacggtaatg atgcacaggc catcgcagtg cttctgatgt aaccgacaat
      961 gctgttcatg cctccaaaaa gctcggaaca acaggatatc tacgctttgc accgggcaag
     1021 gcagcatatt gttaatgtga ccgtttgtca aatcagggga ctgttgcttg attggggaat
     1081 aactatcggc gcggtagttt caagagatcg tcgtgctatg tcaacgccaa gattattggc
     1141 cgttttgggg atgcggacga tttcctggac ttctttatta tgccagccta cattgttggc
     1201 ggtacatttc agggctgtct tcacccagcg ataattttat acgccgctcg ttataccctc
     1261 tgatgtaagc atccacatgc tgcatgaact tttccggcat gatgcccgac catttacgcc
     1321 cataatacat ttccgttttc agtctgccga aaaagccttc gcatgcggca tcatccttac
     1381 gggacattga gtgaataaga cctgcggcat taactctttc cagccagcct ggccagcgat
     1441 aatgcccacc tctgccactg tgtatcatcg gtcgttcgcc agcatttaac gttttgacag
     1501 cgttatccag catagcgtta accagttctg cgtcggggcg tgtactgaga gaccagctca
     1561 caacttttcc atcgaagcag tccaccacct gtgacagcca gacttttcct gcaggaagct
     1621 ggaactccgt tatatctgtc agccatttct gattaggttg gtcctcctta aaatctctgg
     1681 cgataaggtt atccggatcc ggtccgattt ctccgcaata tgagctgtag cggcgacgac
     1741 ggttacggct gacgacaagc tgttccttca ccataagtct gcttacaacc ttttctgata
     1801 gctgcccccc tcatgcctga gcatcgcatg caggcgacga tagccataac actggtaatt
     1861 actgttaact atatctgtca gcatcgtacg tatcgtggcg tatttatcac cggctttcag
     1921 tgcagcccgg tgataaaaat aactgctacg ggcaaggccc agaacatgca gtaattctgt
     1981 caggggatat gtttgtctca gggcatcagc gatatttgtt ttttctctgt tgtttaggtg
     2041 actgatactg atgcccgggt ctaatgagat ggtcaccccc accctgtgag cggtacgctg
     2101 acagggtggg ttattttaga gggcaccatc atgtaaaacg ttgcgcttat tggtatcgat
     2161 ctcggtaaac attctttcca tattcattgt caagataaat ctggcaatgc gcttttgcgt
     2221 aaaaagttta cgcgtacgaa gctgatgcag tttctggcaa catgtccatc ctcagtagtt
     2281 gttatggaag cctgcgctgg tacgcatttt atggcccggc gtatcagcga catcggtcat
     2341 gaagaaaaac ttatctctcc gtaattcgtg cgtccgtttg tcaaaggtaa taagaatgat
     2401 tttattgatg cagaagccat atgcaaagcg gcttcacgac catcaatgcg ttttgtccag
     2461 cccagaacag aaacccagca ggcaatgagg gcattacacc gggtcagaga atcgctcatc
     2521 agagataagg tgaaaaaaac caaccagatc cacggtttct tgctggaatt tggaatcaga
     2581 ctgccgacag gggatgccgc aataaaatgg ttgtctctgg cgttagctga acacgaaata
     2641 cctgaatatc tgagtcgctt gctgatgaaa ttacatgctc actatcttta tcgtattgag
     2701 caaataacag aactggaatc agagttaaac cagtca
//
LOCUS       DBBGLQ010000268         2680 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.269, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000268 DBBGLQ010000000
VERSION     DBBGLQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.269"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..310
                     /locus_tag="ACIYSB_005481"
     CDS             <1..310
                     /locus_tag="ACIYSB_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HGC5432277.1"
                     /translation="NKKYELVIKGISSHPDKVVVTVALESDGYPTLFSPHVVIKLDRV
                     DGAALELYEAEAKKRAKKIFMDAAAGLCEAWSLPEKRLHSEARYTIKINGSDNSIAGF
                     "
     gene            complement(307..546)
                     /locus_tag="ACIYSB_005482"
     CDS             complement(307..546)
                     /locus_tag="ACIYSB_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432278.1"
                     /translation="MKKETFINVTADCSSPNSTTGEIEALKYMIAVMFSVLDQNEKNA
                     IIYQLTEHADNPYIKSNIEMLLPMKDIGKPTETKG"
     gene            671..1204
                     /locus_tag="ACIYSB_005483"
     CDS             671..1204
                     /locus_tag="ACIYSB_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGC5432279.1"
                     /translation="MSTKIRYGLSAAVLALIAAGASAPVILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEAQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1421..1606
                     /locus_tag="ACIYSB_005484"
     CDS             1421..1606
                     /locus_tag="ACIYSB_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGC5432280.1"
                     /translation="MRKLKMKLCALMLPLVVSACGSMSVAPKPCVKPPDPPAWIMQPA
                     PDWQTPLNGIISPSENG"
     gene            1711..2061
                     /locus_tag="ACIYSB_005485"
     CDS             1711..2061
                     /locus_tag="ACIYSB_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGC5432281.1"
                     /translation="MPSRIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLCNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIN"
     gene            2209..>2680
                     /locus_tag="ACIYSB_005486"
     CDS             2209..>2680
                     /locus_tag="ACIYSB_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGC5432282.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDPKKTAKKDEKGLPKIPQHL
                     GSQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNTAGPDDVDPLAELLK"
BASE COUNT          708 a          622 c          723 g          627 t
ORIGIN      
        1 gaataaaaaa tacgaactgg ttattaaagg gataagtagt cacccggata aggttgtggt
       61 tactgtggcg ctggagagcg acggctatcc gacgctgttt tcgccgcatg tggtgattaa
      121 gcttgatcgt gttgacggcg cagcgctgga gctttatgaa gcagaggcga aaaaacgagc
      181 gaagaagatt tttatggatg ctgctgccgg gttatgtgaa gcgtggtcgt tgccggaaaa
      241 gcgcctccat tcagaggcgc ggtataccat aaaaattaac ggttcagata actcaattgc
      301 tggtttttaa ccttttgtct cagttggctt tccaatatct ttcattggta gcagcatttc
      361 gatattgctt ttgatatatg gattatctgc atgttctgtt aattgataaa tgatagcatt
      421 tttttcattc tggtctaata cagaaaacat aactgcaatc atatatttaa gagcctcaat
      481 ttctcctgtt gttgagttgg gggatgaaca gtccgctgta acattaataa aagtttcttt
      541 tttcatatca gatccttaga tggggtagct cagccaacca cccgagcctt aaccggaacg
      601 ccagtgccca ctacactact ggcgggctga ggctttaaca tatccagggt tcagaaaacg
      661 ataaatatcc atgagcacaa aaatcagata cggcctgtcg gctgccgttc tggcgttgat
      721 tgctgcaggt gcatccgctc ccgtcattct tgaccagttt ctggacgaaa aagaaggtaa
      781 tcacactacg gcataccgtg atggctccgg tatatggacc atctgtcggg gtgccacgat
      841 ggtggatgga aaacccgttt ttcccggtat gaaactgtcg aaggaaaaat gcgaccaggt
      901 caacgccatc gaacgggata aggcgctggc atgggtggag cgcaatatta aagttccact
      961 gaccgaagca cagaaagcgg gtattgcgtc attctgtccc tataacattg gccccggtaa
     1021 gtgtttcccg tcgacgtttt ataagcggct gaatgccggt gatcgtaagg gcgcatgcga
     1081 ggcgattcgc tggtggataa aagatggtgg gcgcgattgc cgcatacgtt caaataactg
     1141 ctatggacag gttattcgtc gtgaccagga aagcgcatta gcctgttggg gaatagatca
     1201 gtgagcagag tcgccgcgat tatttatgct ctggttatct gcactatcgt ctgcctgtcg
     1261 tgggcggtca atcattaccg tgataacgcc atagcctaca aagaacagcg tgataaaaaa
     1321 gtcagtgagc tgaagcaggc gatcgccacc atcgctgaca tgcagcagcg tcagcgtgat
     1381 gttgctgcgc tcgatgcaaa gtactcgaga gaattagcca atgcgaaagc tgaaaatgaa
     1441 actctgcgcg ctgatgttgc cgctggtcgt aagcgcctgc gggtcaatgt cagttgctcc
     1501 gaagccgtgc gtgaagccac cggacccacc agcgtggata atgcaaccag cccccgactg
     1561 gcagacaccg ctgaacggga ttatttcacc ctcagagaac ggttgatgac gatgcagaag
     1621 caactggaag gggcacagga gtatatccgc actcagtgca ttaagtagcc tttttatcgt
     1681 ggtaaacatt tcgcagggta tgaggtattt atgccatcac gaatcccacg cgcctgccgt
     1741 aagcgtggat gtgcaggtac aaccacagac agttctggtt actgcgataa acatcgtggc
     1801 gaaggatggg tacagcatca acgcggactg agccgccacc agcgtggcta tggctcgaaa
     1861 tgggatgcca tacgtgcgcg catactgaag cgtgataatc atctgtgtca gaactgcctg
     1921 tgcaatggga gagctgttga agccagaact gtggaccaca tcattccgaa agctcatggt
     1981 ggcacggatg cagacagtaa cctgcagagt ctgtgctggc cctgtcataa agcaaaaaca
     2041 gcgcgcgaac gcatcaattg ataacagctc ccatctgcag gggaggggcg ggtcaaatct
     2101 ctgcaaccca ggctgctcag taccgccgcc ttgcctttcc tcgcatcgcc gcaggttcga
     2161 aaactttttt ttggaaagtt attaaatgat tgataggtaa aaccgattat gtcaggaccc
     2221 ccgaaaaccc cgccacgcct gcatttgata cgaggcaacc cctcaaagcg ccccgttaaa
     2281 gacccaaaaa aaaccgctaa aaaggatgaa aaaggtctcc ctaaaattcc gcaacattta
     2341 gggtcgcagg ggaagtactg gttcaggcga atggcggaag aactgaatgc ggaagggatc
     2401 atttctcagc ttgatgcgcg tgcactcgag ttactggtgg aagcctacac cgaataccgg
     2461 catcactgcg aaacactcga tgttgagggg tatacctacc gcacggaaac gcagaacggt
     2521 gatgtgctga ttaaggcaca cccggctgct gcgatgaaag cggatgcctg gaagcggatc
     2581 cgggcgatgc ttgcagaatt tggtatgtca ccggcaagcc gggcgaaagt aaataccgcc
     2641 ggaccggatg atgttgatcc gctggcggag cttttaaaag
//
LOCUS       DBBGLQ010000269         2670 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.270, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000269 DBBGLQ010000000
VERSION     DBBGLQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.270"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..197)
                     /locus_tag="ACIYSB_005487"
     CDS             complement(<1..197)
                     /locus_tag="ACIYSB_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5432283.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAA"
     gene            complement(339..608)
                     /locus_tag="ACIYSB_005488"
     CDS             complement(339..608)
                     /locus_tag="ACIYSB_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGC5432284.1"
                     /translation="MKELELKKPIIAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(608..964)
                     /locus_tag="ACIYSB_005489"
     CDS             complement(608..964)
                     /locus_tag="ACIYSB_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGC5432285.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVNKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(964..2460)
                     /locus_tag="ACIYSB_005490"
     CDS             complement(964..2460)
                     /locus_tag="ACIYSB_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGC5432286.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATV
                     TLTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIKDAINAVPTLPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKTGTLSELVNAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(2444..2614)
                     /locus_tag="ACIYSB_005491"
     CDS             complement(2444..2614)
                     /locus_tag="ACIYSB_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HGC5432287.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPTEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
BASE COUNT          564 a          800 c          693 g          613 t
ORIGIN      
        1 gcggcgtttt cctgatcggc ataagatttc agggaaagcg tcagccccgc tgccagtccg
       61 ccaccaagcg ccagcccacc ctgtgacgcc tcttccgcct ggcgtttaaa tccccggatt
      121 ttcttttgca ttttcgacag cgcgggagaa agcctgtcga caccggtgat caacgcctta
      181 agctcaaatt cagccatgtg tgcgtttctc ctgctctatc ctgtttgcct gactgaccag
      241 caagggaatt tcactgatcg gcatattcag caattcgaag ggattaatgc gccagtagct
      301 ggcgcagtca aagaagcgat cagtgaggta ttcagccgtc aggcctggag gaaaaaacca
      361 gccacaagcc acgccgctgc attcaggtct gccggagaca tctggtcgac agagctttgc
      421 ggcactttcg ccagccgcac aatgtatttc gacaccacat gcgccagaag tctgacggac
      481 tcatcctgat tcatctggta gggatacccc agctcgcgaa catccttccc ggtgggttca
      541 tcaaactcca gtacggagag tgtctcacca tgagcgataa tcggtttctt taactcaagc
      601 tctttcatta ctggtaatcc ccttcttcac cgtggaactc aagatcaacc gtcccttctt
      661 cggcattatg gttcgcttct ccgtgcagcc aggcggacga cagtacatag acctgaccgt
      721 tcgccagctc ggcagtgatg gtcatctcat cagacgaggt gattttgttc accggaaaat
      781 tcttcggcac cttgaaggtc cctttgacat aaggcgcacg gtgagtttcc ttgcggtcca
      841 ctgaaccgtc caggccgatg atgtcatcat tgaccgtcct gttcatgggc acctcaatgc
      901 cgccggtcag cgatagctgc tgaccgtcaa ttttgaaata acaggttccc ccgatacggg
      961 ccattatgca gactcctctg aatactgaag acggaactgg ttaaccacgg caaaaacacg
     1021 caactggtta acatagtcag gcgggaacag cgtgttcagg cggttcggat cgctggcatc
     1081 acgctccaca accaggtact gcttaaacag ttcgtagttt tccacgatcc ccgcacgctc
     1141 aagctgacgg taggttgcca gcagttcccc tttgattacc gccggggtga caatcgcctg
     1201 accgggacca aagcgggtac cgtcgctggc aagcttgtga cgcccgtact tactggtaat
     1261 gacggatttc agtttgcgca gtacatacgc gctggtatgc agcgtctcgc tgtcgaggta
     1321 gctgttatcc gcaacaccgt aagcattttt cctgtacgtg gtgacatcac gctgaatgcg
     1381 cagcaccccg ctttcgacat acgccgttgc cacgccatga gacagcaggg tctgctgctc
     1441 ggtcatcgtg aaccgtttcc ccttcggcgc aggcaacata cctaccagct caccggtctg
     1501 cgtgggacgt gccggatcgt tgcggataaa caccgctgcg cgggcggtac ggcttgccgc
     1561 cagctcgtcg gcaggcgtct gggtctcttt ttcgtacccc gccagggtga tgtgctgctg
     1621 gttaaactgg tcacctgcgt tcaccagttc tgacagtgtg ccggtctttg ccgtatacac
     1681 atgaccatac agctgacgcg catagctcca gcgaccgctg gtatcgttca tctcggtcac
     1741 cagcgtgtta acggaggccg tgtcgttgaa cggcagaccg atataatcaa acggctcatc
     1801 cgccattgca gccaccgcgc cggtgagaac cggagcgccc gttccggcgg tccccgtcgc
     1861 cacggcaatc tgtacgcccg ctggcagtac ttcgccccca ccgaagccgt agtaattgag
     1921 gctgacagga atttcattcc cgcaaagccc cttatgacgc gcggtcagcg tgacaacacc
     1981 agccgaagat gaagctgtaa acggcagagt cggaacggca ttgatggcat ctttgatact
     2041 gctggcaatc gtcgtgacgt tatcgccgtt ggtcaccgga gcctgcacgc gggtacgtcc
     2101 cacatagaca ttcaccgtgc cgctttcggt tgcttccccg gtcaccgtca gcgtaaccgt
     2161 tgccgccgcg cctgtggctt ccggaacggc aatcacatac agctcaccaa acgggtcggt
     2221 ctggcgataa gcctcgacca tacgcgccag ctgacttccc gcaccacaaa tctggcgtgc
     2281 atagtctgcc gacggcatca gcaccagact gttggcaaca atctctgcac cgttattggc
     2341 atgaccaatc agcagcgatg ctccgctgtc ctgtgcagta ttcgccgcct ggttatccat
     2401 ttccgcataa aacagcggaa ccagcgtatt cgacggaatg gtgttaaagc ttatcgtcat
     2461 cggtgttcac ctttttattc acgcgccgga tatcacccgc tgcttcacgg cgcagccagt
     2521 agttgttctc gtcaacattt cgcccttcgg tgggcaaaag gtcaccgcgg gcagggtcag
     2581 gcactgaccg ccctttaaca ggtttcacaa acatgaagat tctcaggaag gaagggttat
     2641 ttcggtgtga tgttcgatat cgccgtcagg
//
LOCUS       DBBGLQ010000270         2615 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.271, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000270 DBBGLQ010000000
VERSION     DBBGLQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.271"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(145..1113)
                     /locus_tag="ACIYSB_005492"
     CDS             complement(145..1113)
                     /locus_tag="ACIYSB_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGC5432288.1"
                     /translation="MSEKVNKITMIHDGAFKKTLEDPAAVRDFLEQVLTHDQISRCNL
                     DTIEVEKNSFVTESLRQHACDVLISMKTNDGRDGYIYTLIEHQSSPDKFIPLRMMRYI
                     LAVMEQHVEKHKCAPVVIPVLFYHGAMRPYPYPLNWVDCLDDPEYGREIYGESKSFSL
                     VDVSTLDDDEIEQYRRMAALMYTMKSGTQGDLIELIGKSITLTDKYGSSIHLNTVLTY
                     LMELYQMDVAELFEAISTHYPSHKGVIMTIAEQLEEKGRVEGLEKGLEKGLEKGRAEG
                     RAEERQKALAETYASVRRMSDMGMSTEVIKQALHLSDEQIQEALNN"
     gene            complement(1160..1717)
                     /gene="ssb"
                     /locus_tag="ACIYSB_005493"
     CDS             complement(1160..1717)
                     /gene="ssb"
                     /locus_tag="ACIYSB_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGC5432289.1"
                     /translation="MASRGVNKVILIGHLGQDPDIRYFPNGGGAVANLSLATSDTWRD
                     KQSGEMKEVTEWHRVVLYGKLAEVAGEYLHKGSQVYIEGQLKTRKWFDQQNGVDRYST
                     EVVVGVNGVMQMLGSSRQQGASGNGASAPAQGGQGHPPKTTGQTGRATRTTSTGNGAP
                     PMDFDDDIPFMGLGYGMERRIIHAM"
     gene            complement(1730..1933)
                     /locus_tag="ACIYSB_005494"
     CDS             complement(1730..1933)
                     /locus_tag="ACIYSB_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432290.1"
                     /translation="MTSTNGILAAAAGYTLPLEILKSGRGYYIGTQCSEGPVSRESEE
                     YFKKYEQAERALKNGTWTQRHAW"
     gene            complement(2033..2497)
                     /locus_tag="ACIYSB_005495"
     CDS             complement(2033..2497)
                     /locus_tag="ACIYSB_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HGC5432291.1"
                     /translation="MSATNSTSAKTEYFNLTMKGLGYLSNVRQVNHQNGSFLSCVINA
                     LSGPTDNPSYTRFDVTVAGKEATTLISRCQKAVDEDRKVLLGFTLSNLATDIFTLNKG
                     EHAGEQRVSLKARLIKVDWIKIGQEQVYKAEKAESQPPSHGTTKREYAEDSF"
BASE COUNT          633 a          704 c          580 g          698 t
ORIGIN      
        1 gcaaaatcca tctggtaaag ttccatcaca atttaccaga ctgattgata aaaaaacgcc
       61 tgcacaatgg caggcgctgt gttcacaggg catcacacgt tctgacgcat cagtcgcgct
      121 ctgccacatc attacgatgc gaggttaatt attaagcgct tcctgaatct gttcatcaga
      181 aagatgaagt gcctgcttaa taacttccgt tgacatcccc atatcgctca tgcgacggac
      241 agatgcatag gtttctgcaa gcgctttttg acgctcttca gcacggcctt cagcacggcc
      301 tttctcaagg cctttctcaa ggcctttctc cagaccttca acccgacctt tctcttccag
      361 ttgttccgct atcgtcataa tcaccccctt atggctggga tagtgtgtgg atattgcttc
      421 aaaaagttca gcaacatcca tctggtaaag ttccatcaga taagtaagca ccgtattcag
      481 gtgaatgcta ctgccatact tatccgtcag tgtgatcgat ttaccaatca gctcaatcaa
      541 atcaccttga gttccggact tcatggtgta catcagcgcc gccatccggc gatactgctc
      601 aatttcatca tcatcaagcg tactgacatc caccagactg aatgactttg actcgccata
      661 aatctcccgt ccgtactccg ggtcatccag acaatccacc cagttcagcg gatacggata
      721 tggccgcatt gcaccatgat aaaacaacac ggggatcacc acaggtgcac acttatgctt
      781 ttcaacatgt tgctccatta cagccaggat atatcgcatc atacgcagcg ggatgaattt
      841 atccggcgaa ctctggtgct cgattaaggt ataaatgtat ccgtccctgc cgtcgtttgt
      901 cttcatgctg atcaaaacat cacaggcatg ctgacgcaaa ctttccgtca cgaacgaatt
      961 cttttcaact tctattgtat caagattaca gcgggatatc tgatcgtgtg tcaagacctg
     1021 ttcaagaaag tcccttactg cggcgggatc ttcaagcgtc ttcttgaacg ccccgtcatg
     1081 aatcatagtg attttgttaa ctttttcact cacggaacct ccggataaaa aagtggggcg
     1141 taacgccccg tggttaatat tacatggcat gaataattcg tcgttccatg ccatacccga
     1201 gccccataaa ggggatatca tcgtcaaagt ccattggtgg agcaccatta cccgttgacg
     1261 tcgtccgggt ggcacgacca gtctgtccgg tcgtcttcgg cggatgtccc tggccccctt
     1321 gtgccggtgc tgaagcacca ttaccggaag cgccctgctg acgcgatgaa cccagcatct
     1381 gcatgacacc attcacgccc acaacaacct cagttgaata acggtccaca ccattctgct
     1441 ggtcaaacca tttacgggtt ttaagctggc cttctatata cacctgcgac cccttatgca
     1501 ggtactcgcc ggcaacttca gccagttttc catagagaac aacgcggtgc cattcagtaa
     1561 cttccttcat ttcaccgctc tgtttatccc gccaggtgtc gctggtcgcc agtgacagat
     1621 tcgccacagc gccaccgcca ttggggaaat accggatatc cggatcctgc cccagatgtc
     1681 ctatcaggat aactttgttt acgccgcgtg aagccatagc acctcctcat taccaggcat
     1741 gacgttgtgt ccaggtgcca tttttcagcg cccgctcagc ctgctcatat tttttgaaat
     1801 actcttctga ttcacgggat accggtcctt cagaacactg agtgccaata taatatccgc
     1861 gcccactttt gagtatttcc agaggtaatg tataacctgc cgcagctgcg agaataccgt
     1921 ttgtagaagt cataatttgt ttccttaaag ttcagtaaaa acaataagaa aacagacctc
     1981 cctgatggtg gtctgttttc tttatcaggg aggcagtgag aaaaatacgc gatcagaagg
     2041 agtcttctgc gtattcacgt tttgtggtgc catggctcgg tggctgggac tccgcttttt
     2101 cagccttata gacctgctcc tggcctattt ttatccagtc caccttgatc aggcgggctt
     2161 tcaggctgac gcgttgctca ccggcatgct cccccttgtt cagggtgaaa atgtcggtgg
     2221 caaggttgct taacgtaaaa ccaagcagta ccttgcggtc ctcatccact gctttctggc
     2281 agcgggagat cagggtggtc gcctctttac cggcaaccgt gacatcaaaa cgcgtatagg
     2341 acggattgtc cgttggtcca ctcagtgcgt taatcacgca gctgaggaac gaaccgttct
     2401 gatggttaac ctgacgtaca ttgctgagat acccaagacc tttcatggtc aggttgaagt
     2461 attcagtttt tgcagaagta gagttagttg cagacatgat gatttctcca tggagtaaaa
     2521 aaagatatga cggagaacat caccagccct gacgggagtg atagttcccg tcggggatgc
     2581 cggaatcagc tgaatttccg gctgtctgtg cagtt
//
LOCUS       DBBGLQ010000271         2584 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.272, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000271 DBBGLQ010000000
VERSION     DBBGLQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.272"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            37..1008
                     /gene="yfbL"
                     /locus_tag="ACIYSB_005496"
     CDS             37..1008
                     /gene="yfbL"
                     /locus_tag="ACIYSB_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HGC5432292.1"
                     /translation="MKKIIFAFIILFVFLLPMIIFYQPWVNALPSTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFVSSGARVTSQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSASSYENDQLTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSAPGSQNYPYPAMSWLY
                     PDRGDFIAVVGRIQDINAVRQVKAALLSSQDLSVYSMNTPGFIPGIDFSDHLNYWQHD
                     IPAIMITDTAFYRNKQYHLPGDTADRLNYQKMAQVVDGVITLLYNSK"
     gene            1111..1614
                     /gene="yfbM"
                     /locus_tag="ACIYSB_005497"
     CDS             1111..1614
                     /gene="yfbM"
                     /locus_tag="ACIYSB_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGC5432293.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRNELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1887..>2584)
                     /gene="yfbN"
                     /locus_tag="ACIYSB_005498"
     CDS             complement(1887..>2584)
                     /gene="yfbN"
                     /locus_tag="ACIYSB_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YfbN"
                     /protein_id="HGC5432294.1"
                     /translation="RKLRKNVPVGIQVARRLLERTGGDVDEAIKLFHIDQINILTAKA
                     DVTHQEAENVLLATNYDIAEALRRIDEQRYTLTELILRKNKDAGDALNNIALAIEYEW
                     DLKRKFWFGFADIQLLPPVLQTFMLVYEWHEYVGWEGMECGIFFESDHTHQQLQALGL
                     LELAQKMVTARIRYDELKDKAENFHEITEDDIFKMLIIHCDQLAREVDSILLQFVKDN
                     IDVFPCRHNRHEL"
BASE COUNT          736 a          556 c          537 g          755 t
ORIGIN      
        1 aatgattcaa caacgcaacc cattaataat ttgcatatga aaaaaataat ctttgctttt
       61 attatattat ttgtgttttt actccctatg attatttttt accaaccctg ggtcaatgca
      121 ttaccgtcaa cgccccgaca cgcaagtcct gagcaattag aaaaaacagt tcgttatctt
      181 acacaaactg tgcatccacg tagtgccgac aatattgata atctgaatag gtccgcagaa
      241 tatataaaag aggtctttgt cagtagcggt gccagagtta cctcgcagga cgtccccatt
      301 acgggtggcc cctacaaaaa cattgttgct gattatggtc ctgccgatgg accgctgatt
      361 attattggtg cgcattatga ctctgccagc agttatgaaa acgatcaatt gacctatacg
      421 ccgggcgcgg atgataacgc cagcggtgtg gcaggattac tcgaactggc acgtttgtta
      481 catcagcaag taccgaaaac aggcgtgcag ctggtcgcct atgcgtcgga agaaccgccc
      541 ttctttcgta gcgatgaaat ggggagcgcg gtgcatgcag cttcgcttga gcgtccagtg
      601 aaattaatga tagcactgga gatgattggc tattacgact ctgcgcctgg aagccagaat
      661 tacccttacc cggcaatgtc ctggctttat cccgatcggg gagactttat tgccgtggtc
      721 ggcagaatac aggatatcaa cgccgttcgt caggtaaaag cggcattgtt gtcatctcag
      781 gatttatctg tttattctat gaatacccca gggtttattc ccggtattga tttctctgac
      841 cacctgaatt attggcaaca cgatattccc gccataatga ttactgacac cgctttttat
      901 cgtaataaac aataccactt gcccggtgat accgcagaca gattgaatta tcagaaaatg
      961 gctcaggtag tggatggtgt tataactttg ttatacaaca gtaaataata aaaatttctc
     1021 gaggcgggtc cagaaaaagc gacatgcaca ttttattttc cctcatctgt tttaagatgc
     1081 gaaacatgaa attcaacgga ggaaaaataa atgggaatga ttggctattt tgcagaaatt
     1141 gactcagaaa agattaacca actgcttgag tctactgaaa aacctttgat ggataatata
     1201 catgacacgc tttcaggatt acgccgactg gatattgata aacgctggga ttttttacac
     1261 tttgggctga caggcacctc tgcttttgat cccgcgaaga atgatccgct gagtcgtgcc
     1321 gtgcttggtg aacacagcct cgaagatggc atagatggct tccttggatt gacctggaat
     1381 caggagttag ccgcgacaat cgaccgactg gaatcactgg atcgaaatga actgcgtaaa
     1441 cagttttcca ttaagcgact caatgaaatg gagatttatc cgggagtgac cttttcagaa
     1501 gagttagagg ggcaactctt tgcctcgatc atgcttgata tggaaaagct gattagcgcg
     1561 tatcgacgta tgctgcgaca agggaatcat gcgctgacgg ttattgttgg gtgatatagc
     1621 ttgatgcctg ggggagaaag acccattagt taagatttac gccaggatca tcttgaaaaa
     1681 atctttaacc atggtccggt ttttatcccc ttgtataaat attactaaat gcttgcttag
     1741 tcgcattgtt aaatgcaata ccgtatattc ggtatgtaac agcgatatcc tctgtgctgg
     1801 caacgatctg tcatgcttga tgaaaacgga attgaacctg tcattaccag gtagataaaa
     1861 agcctgttca attttgggat tattttttat aactcgtgtc tgttatggcg acacgggaaa
     1921 acatcaatat tgtccttcac aaattgcagt aatatagaat caacctctct tgcaagttgg
     1981 tcacaatgta ttataagcat tttgaatatg tcatcctctg taatctcgtg aaaattttcg
     2041 gccttgtctt ttagctcatc atatcggatt cttgcagtaa ccattttttg ggcgagttct
     2101 aataacccca aagcttgcaa ttgctggtga gtatgatcac tttcaaaaaa tatcccacac
     2161 tccattcctt cccaaccaac atattcatgc cattcataaa ccagcataaa tgtttgtaaa
     2221 actggcggta ataattggat atctgcaaaa ccaaaccaaa acttacgctt gagatcccac
     2281 tcatattcaa tcgccaatgc tatattgttt aaggcatctc cggcatcctt atttttacgc
     2341 aaaatcaatt ccgtcagtgt atagcgttgt tcatcaatac gccgaagagc ttctgcaata
     2401 tcatagtttg tagccaacaa aacattctct gcttcctgat gagtgacgtc tgcttttgcc
     2461 gtcaatatat tgatttggtc aatatgaaac aacttgatgg cttcatcaac atcaccgcca
     2521 gtcctctcta acaaacgtct ggcaacctgg ataccgacag gtacgttctt ccgaagttta
     2581 cgaa
//
LOCUS       DBBGLQ010000272         2568 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.273, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000272 DBBGLQ010000000
VERSION     DBBGLQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.273"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..485)
                     /locus_tag="ACIYSB_005499"
     CDS             complement(<1..485)
                     /locus_tag="ACIYSB_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HGC5432295.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIEALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     D"
     gene            complement(577..1071)
                     /locus_tag="ACIYSB_005500"
     CDS             complement(577..1071)
                     /locus_tag="ACIYSB_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432296.1"
                     /translation="MRIFVFFISALLSFNLAAEECKFSFNESELISSIGIAPVKQEII
                     KDEGITKRQYEFRRELSSEEMLSDDADEKYEPQFYISVYNPLCPQKVIVWFFKDNKNT
                     MDLSNEVLAGRAFKYLTGVNESIFENKMKKFLKVQSFESFDERTDSKFIKSGDIYSID
                     VQLR"
     gene            complement(1171..>2568)
                     /locus_tag="ACIYSB_005501"
     CDS             complement(1171..>2568)
                     /locus_tag="ACIYSB_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5432297.1"
                     /translation="EFAAKMQDATGTASEDMMGLFDTIQKAFYLGVDDTNMLSFFTKT
                     SSVLKMVNKDGLQAAQSLAPISVMMDQMGMNGESAGNALRKVIQSGLSVKKIRDVNKV
                     MARQKLGVQLDFTDGKGSFGGLDNMFRQLAKLRKLTDVKRTGVLKAIFGDDAETLQVV
                     NALIDKGKDGYDQIQQKMNKQASLNKRVQAQLGTLSNLWEAMTGTATNGLAAIGGAFS
                     GDAKNITQWLGELGEKFTKFADENPRVIRGVVGLAAGLAILKLGLMGVGSAISIVSRI
                     MSMTPIGMIATAIALAAGLIITNWDVVGPYFKKLWETIGPYFEAGWELLKKVFAWSPL
                     GMVINNWGPVVKWFQDMWDKLKPIIEWFTDSSGDTVDAINSAQWGAGAYDAYGTGIPA
                     RGYTPYPAVDPAQSNNASDATGSNPFMINKASVPKVDGEIKVSFVNSPPGMRVMETRS
                     SGFDVSHDVGYTRFR"
BASE COUNT          697 a          692 c          492 g          687 t
ORIGIN      
        1 aaatccgcca caccatcgat actgaaaccg ctgaagctgt cactgatgca gtcatccagt
       61 gcagaacagg atgacatcag cgtttgcgcc gtcgccgcac ctgatgtggg gtaagagagt
      121 tctcctgctt cgacaaactt caggtcaaag cggacaatac gcccttcact tttcgatgtg
      181 ctgacccgaa cttccccgtc aacacagact ttcagctcac catatgtcgg gtggacaagc
      241 gtgccgggac cgggtttatt cagcgcttca atcaggcgat cgcgctggtc aaagcagtca
      301 tctcccacca cataagctgt gatggacggg cggaaagtga cttttcccag gtcttcggta
      361 tagggcttgt cgcggttcgg atattcgtgt gtttccacac ggcgaccggt tcccgcactt
      421 tcttcttcaa ccttaaacgg tacgccgcga aatgacgcat cctgaagcct gtctttccac
      481 gtcatataaa ctccggatac aaaaaaaccc gccaaatctg ctttgtcagt tatttacatc
      541 gcagaagatg tggcgggaac ctaatatttt taattactat ctgagttgaa catcaatgga
      601 ataaatatca ccactcttta taaatttaga atctgtcctt tcatcaaaag attcaaatga
      661 ctgtaccttt aaaaactttt tcattttatt ttcaaaaata ctttcattaa caccagttaa
      721 atacttgaac gctctaccag caaggacctc attacttaaa tccattgtgt ttttattgtc
      781 tttgaaaaac caaacaataa ccttttgtgg gcataatgga ttataaacag atatataaaa
      841 ctgcggctca tatttttcat cagcgtcatc actaagcatt tcttcagaag ataattctct
      901 tctgaattca tattgccgct tagttattcc ttcatccttt attatctctt gcttaactgg
      961 tgcaatacct atagaagaga ttaattctga ctcattaaag ctgaacttac actcttccgc
     1021 agccaagtta aaagataaaa gtgcagatat aaaaaaaaca aagatacgca taatcatccc
     1081 ttcaatcatt tgtaaggaat gattatatta actacttaaa gctgaaaacc caaattatgc
     1141 cagacaaaaa cacattaatc attttgtaca ctacctgaac cgcgtatagc caacatcatg
     1201 gctgacatca aaaccgctgg atcgcgtttc cataacccgc atacccggag gcgaattcac
     1261 aaaagatacc ttgatctcac catcaacttt tggcacagaa gctttgttaa tcatgaaggg
     1321 attcgagcct gtggcatcgg aggcgttgtt tgactgagcc ggatccaccg ccggataagg
     1381 tgtgtatccc cgcgccggta ttcccgtccc ataagcatca taagcacccg cgccccactg
     1441 cgcagagtta atggcatcga ccgtgtcacc ggaactgtcg gtaaaccact caataattgg
     1501 cttcagcttg tcccacatat cctgaaacca cttaacaacc ggtccccagt tattgatcac
     1561 cattcccagc ggcgaccagg caaaaacttt cttcagaagt tcccagccag cctcaaaata
     1621 aggaccaatg gtttcccaga gcttcttaaa ataaggtccg acaacatccc agttagtgat
     1681 aattaatccc gcagccagag caatcgccgt cgcaatcatg cctatcggcg tcatcgacat
     1741 aatcctgctg acaatactga tggcactgcc cacgcccatc aatcccagtt tcagaatcgc
     1801 aagaccggca gcaagcccga cgacgccgcg aataacccgg ggattttcat ccgcaaattt
     1861 cgtgaatttc tcccccaact cccccagcca ttgcgtgata tttttagcgt caccagaaaa
     1921 tgcgccgcca atagccgcaa ggccgttagt tgcggtccct gtcattgcct cccacaggtt
     1981 ggacagcgta ccaagctgtg cctgaacacg tttattcagg ctggcctgtt tattcatctt
     2041 ctgctggatc tgatcgtagc catcctttcc tttatcgatt agtgcattga ccacctgaag
     2101 ggtttcggca tcatcaccaa atattgcctt aagtacacct gttcgcttaa cgtcggtcag
     2161 ttttcgcagc tttgccagtt gcctgaacat gttatcaaga ccgccaaaac ttcctttgcc
     2221 gtcagtaaaa tcgagctgta ccccgagttt ctggcgggcc ataactttat taacgtccct
     2281 gattttctta acgcttaatc cggactggat aacttttcgc agggcattac ctgccgactc
     2341 cccgttcatc cccatctgat ccatcatgac gctgatgggg gcaaggctct gcgcagcctg
     2401 aagaccatcc ttgttcacca tcttcagaac agaactggtt ttagtgaaga aggacaacat
     2461 gttggtatcg tcaacgccca gataaaacgc cttctggata gtgtcgaaca gccccatcat
     2521 gtcttctgac gccgttccgg tagcatcctg catctttgca gcaaactc
//
LOCUS       DBBGLQ010000273         2567 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.274, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000273 DBBGLQ010000000
VERSION     DBBGLQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.274"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..231)
                     /locus_tag="ACIYSB_005502"
     CDS             complement(<1..231)
                     /locus_tag="ACIYSB_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGC5432298.1"
                     /translation="MKQRLASAQVALDTLRARRGDTWYPVFHLAPPAGWMNDPNGLIY
                     FSGRYHAFFQHHPASEHQGPMHWGHATSPDMLH"
     gene            complement(228..1505)
                     /locus_tag="ACIYSB_005503"
     CDS             complement(228..1505)
                     /locus_tag="ACIYSB_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGC5432299.1"
                     /translation="MNTVSTHKNPDFWIFGLFFFLYFFIMATCFPFLPVWLSDIVGLS
                     KTDTGIVFSCLSLFAISFQPLLGVISDRLGLKKHLMWIISLLLVLFAPFFLYVFAPLL
                     RFNIWAGALSGGIFIGFVFSAGAGAIEAYIERVSRSSGFEYGKARMFGCLGWALCATT
                     AGLLFNVDPSLVFWMGSGCALLLVLLVALARPGTSQTAMVMNALGANSPVISAGMVFS
                     LFRMRQMWMFVLYMIGVACVYDVFDQQFATFFRAFFDTPQAGIRAFGFATTAGEICNA
                     IIMFCSPWIINRIGAKNTLLVAGLIMTIRITGSAFATTATEVVILKMLHALEVPFLLV
                     GAFKYITGVFDTRLSATIYLIGFQFSKQLAAIFLSTFAGHLYDRMGFQNTYLVLGGIV
                     LAVTVLSAFTLSSTRKTAAPSAETATAQTEVRL"
     gene            complement(1560..>2567)
                     /locus_tag="ACIYSB_005504"
     CDS             complement(1560..>2567)
                     /locus_tag="ACIYSB_005504"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGC5432300.1"
                     /translation="WVEPEMINPDSDLFRRHPDWVLSMPGYTQPTGRHQYVLNLNIPE
                     AFAYIYERCLWLLGEHPVDYVKWDMNRELVQAGHEGRAAADAQTRQFYRLLDLLRERF
                     PRVEFESCASGGGRIDFEVLTRTHRFWASDNNDALERCTIQRGMSYFFPPEVMGAHIG
                     HHRCHATFRQHSIAFRGLTALFGHMGLELDPVTAGEEESAGYRRYASLYKEWRQLIHT
                     GTLWRVDMPDAATQVQGVVSPDQSQALFLVSQLAMPDHTLPGALRFPGLAPQARYRLR
                     VVDHPDIQLVGEGGHTMRRLPGWMNQTSDISGEWLAQAGIQLPVLDPESAMLIALERL
                     A"
BASE COUNT          651 a          727 c          724 g          465 t
ORIGIN      
        1 gtgcagcatg tccggactgg tggcatgtcc ccagtgcatc ggcccctgat gttcacttgc
       61 cggatggtgc tgaaagaatg cgtgatagcg accgctgaaa taaataagcc cgttcggatc
      121 attcatccat ccggcaggtg gagccagatg aaaaaccggg taccaggtat caccacgacg
      181 ggcgcggagt gtatcaagtg caacctgtgc agaggcaaga cgctgtttca taagcgaacc
      241 tccgtctgtg ctgtagcggt ttctgctgac ggggcagccg ttttacgggt actgctcagg
      301 gtgaaagccg ataacacggt gaccgcaagg acaataccgc ccagaaccag ataggtgttc
      361 tggaacccca tgcggtcata caggtgacct gcaaaggttg agaggaatat cgccgccagt
      421 tgtttggaga actgaaagcc aatcagataa atggtggccg acagccgggt atcaaacacg
      481 ccggtgatat acttgaacgc accaaccagc aggaatggga cttcaagggc atgcagcatt
      541 ttcaggatca ccacttcagt agccgtggtg gcaaaggccg accctgtgat gcggatggtc
      601 ataatcagac ccgcgacaag cagtgtgttt ttggcaccaa tgcggttgat tatccagggg
      661 gagcagaaca tgatgatggc attgcaaatc tcgccagccg tggtggcaaa accaaatgcc
      721 cggatccctg cctgcggcgt gtcaaaaaat gcacggaaga acgtggcaaa ctgctggtca
      781 aacacgtcat agacgcaggc caccccaatc atgtaaagca cgaacatcca catctgacgc
      841 atacgaaaca gactgaacac cattccggcg gaaatcacgg gcgagttggc acccagcgca
      901 ttcatgacca tcgctgtctg actggttccg ggacgcgcca gcgccaccag aaggaccagc
      961 agcaatgcgc agccggaccc catccagaat accagggacg gatccacgtt gaaaagcagc
     1021 cctgccgtgg tggcacacag cgcccagccc agacagccga acatccgggc cttaccgtat
     1081 tcaaatccgc tgctgcggct gacacgttca atataggctt cgatggcacc ggctcccgca
     1141 gaaaaaacga agccgataaa aatcccgccg ctcagtgctc cggcccagat gttgaaccgc
     1201 agcagcggcg caaaaacgta cagaaagaac ggtgcaaaca ggaccagcag caggctgatg
     1261 atccacatca ggtgtttttt caggccaaga cggtctgaga tgacccccag cagcggctgg
     1321 aagctgatgg caaacaggga gaggcatgaa aaaacaatcc cggtatccgt tttactcagc
     1381 cccacaatgt ctgacagcca gacgggcaga aacgggaaac aggttgccat gatgaaaaag
     1441 tagagaaaaa agaacagacc aaatatccag aaatcggggt ttttatgagt actgacagta
     1501 ttcattattg ttatcctcac gggactgagt gcagggagcc gtgaccggct ccggtccttt
     1561 tatgccaaac gttcaagggc aatcagcatg gcgctttccg gatccagcac gggaagctga
     1621 ataccggcct gcgccagcca ttccccactg atgtcggagg tctggttcat ccagccgggc
     1681 agcctgcgca tggtatgccc gccttcaccg accagctgaa tatccggatg gtcaacaaca
     1741 cgaagccggt aacgggcctg tggtgccagc cccggaaaac gtagagcacc cggcagggtg
     1801 tgatccggca tggcaagctg gctcaccagg aagagcgcct gcgactgatc cgggctgacc
     1861 accccctgca cctgtgtcgc cgcatccggc atatccaccc gccatagggt gcccgtgtgg
     1921 ataagctgcc gccattcttt gtagagcgag gcatagcggc ggtaaccggc ggattcctct
     1981 tctccggccg ttaccggatc cagctccagt cccatatgac cgaacagggc cgtcagaccg
     2041 cgaaaggcaa tgctgtgctg ccggaaggtg gcatggcagc gatgatgacc gatgtgtgcc
     2101 cccataacct ccggcgggaa aaagtaactc atgccacgct gtatggtgca gcgctccagg
     2161 gcgtcattgt tgtcagaagc ccagaagcgg tgcgtgcggg tcagaacctc aaagtcgata
     2221 cgtccaccgc cggaagcgca ggactcaaac tcaacacggg gaaaacgttc gcgcaggaga
     2281 tccagcagcc ggtaaaactg acgggtctgc gcgtccgccg ccgcccgacc ttcatgcccg
     2341 gcctggacca gctcgcggtt catgtcccat ttcacatagt caactgggtg ttcgcccagc
     2401 agccataaac agcgttcata aatgtaagca aaggcctcag gaatgttcag gttgagaaca
     2461 tactgatgtc tcccggttgg ttgtgtgtac cccggcattg acagtaccca gtcaggatgg
     2521 cggcgaaaga ggtcagagtc cgggttaatc atttccggtt ccaccca
//
LOCUS       DBBGLQ010000274         2563 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.275, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000274 DBBGLQ010000000
VERSION     DBBGLQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.275"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>2563)
                     /locus_tag="ACIYSB_005505"
     CDS             complement(<1..>2563)
                     /locus_tag="ACIYSB_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGC5432301.1"
                     /translation="GSSKGHTPREAKDNLKSTQLLSVIDVISEGPIQGPVDGLKSVLL
                     NGTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTIT
                     SANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGRWLTEKDITIKGKTTSQY
                     LASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALVGVQ
                     VDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNPAWCLWDM
                     LTHPRYGMGKRVGAADVDKWALYVIGQYCDQSVPDGSGGTEPRITCNAWLTTQRKAWD
                     VLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKDR
                     HNAVEVNWSDPDNGQETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIK
                     TELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREI
                     TLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQR
                     LFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSGTVNGVTPPAVQHLTVE
                     VTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQLAP
                     GNYRLTVRAVNARGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQ
                     FEFWFSEKRITDIRQVETTARYLGTGTQWLVSGQNIKPGHDYYFYIRSVNTVGKSAFV
                     EAVGRASNDPAGYLNFFRGAINKTHLGREINERIDASARRTEVEQLENAINREMTQ"
BASE COUNT          487 a          830 c          671 g          575 t
ORIGIN      
        1 ctgcgtcatt tcgcggttaa tcgcattctc cagttgctca acttcagtgc gccgtgcaga
       61 ggcatcaatg cgttcattga tctcccgtcc cagatgcgtt ttatttatcg cccccctgaa
      121 aaaattaagg taaccggcag gatcattact cgcccgaccg actgcctcca cgaatgccga
      181 tttaccgaca gtgttaacac tgcggatata aaagtaataa tcatggccag gcttaatatt
      241 ctggccggag acaagccact gagttccggt accaagataa cgggctgtgg tttcaacctg
      301 cctgatatcg gtaatccgct tttccgaaaa ccagaactca aactgcaccg tcggatcata
      361 aacggcaaga tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct caatccgcga
      421 cggcgctgcc ggtgcggcaa tccggaacga taccgacgcc gggtctccct gctgcccccg
      481 ggcatttact gcccggactg tcagcctgta gttccccggc gccagctgcc tgaagcggta
      541 tgtggtttcc gtcgtccggg ccgtgctgac cagccgctca ctgccgtcat ccgctgccac
      601 ggtcaggcga agcatgaaac tcacgccttt caccaccttc ggcgtgtccc agcgggccag
      661 tacctgatac tccccgctgt ctgcggtgac ttcgacagtc aggtgctgca ctgctggcgg
      721 cgtgacacca ttcaccgtgc cgctctggtc accgtcaaag tgtgccccgt tatccacgat
      781 gccctctttt tccggtacat gctgcacggc agtgatggca tacgtgccgt cgtcgttctc
      841 acggatactc acgcagcgga acaggcgctg gcgcagcgtc ggcagcttca gcccccacac
      901 gctgtactcg gcaacgccgt caggaacccg gctcactttt accttcacgc cgtcggtgac
      961 ggactgaacc tccacgctga ccggattgcc acttccgtca accaggctta tcagcgtggt
     1021 accggaggat ggcagcgtga tttcacggtc gagcgtcagc gtccgggtct ggctgttcac
     1081 cgccagcaca cgaccaccgg tgctgatacc ggcatagtca tcatcacaga tttcaatgac
     1141 atcgcccggt acatggcgaa gcccttcagc acccacgctg aaatccacgg tctgcgtctc
     1201 cagcagttct gttttaatca gccacagccc ggcgcggtgt gcctgccccc ggctggtaca
     1261 gccaaaggca tccatcttcg tgacgttacg accgtaacgg gcaatggcct gcgtatcttc
     1321 aacaagctct gtcgccgtct cctggccgtt atccgggtca ctccagttca cctcaacggc
     1381 attatggcgg tccttcaggg cgctgaagct gtagcggaac ggcgcgccat catccggcat
     1441 caccacatta ctgcggttat aggtccacac cttatccgac ggtcggtcct gcacgaacgt
     1501 cagcgtctgc ccgttccata ccggcataca gcgcatcgcc gagcagaaat cactgagcac
     1561 atcccacgcc ttacgctgtg tggtcagcca ggcattacag gtgatgcgcg gctccgtgcc
     1621 accagagccg tccggcactg actggtcgca gtactggccg atgacataca gcgcccattt
     1681 atccacatcc gctgcgccaa cacgtttccc catgccgtag cgcggatggg tcagcatatc
     1741 ccacagacac caggccgggt tgttgctgta tgccggttta aacgttccgt cccagatacc
     1801 gctgtattgc cgcgtctgcg ggttatagtt cgacggcacc tgcagaatgc gcccgcgcag
     1861 atgataatta cggctcacct gctggctgcc gaactgctcc gaatccacct gcacaccgac
     1921 cagtgccgtg ttcgggtagc actgtttcac atcgataatt tcggtgtatg acgaccagag
     1981 cgttttgttc tgcagctggt ctgtggtgct gtccggcgtc atcctgcgca tccggatact
     2041 gaacgggcgc ggcggcaggt tatccaccac caccgaagcc agatactgcg aggtggtttt
     2101 tcccttaatg gtgatgtctt tttccgttaa ccagcgaccg ttacgctgta tctgaaccag
     2161 caggcggact tccgacggat tccggtcccc ctttgaggtg gtttccacca gtgcctgcac
     2221 accgaaggtc aggcgcagac ggtcgatgtt tgccgatgtg atggtccggg taatcggcgt
     2281 gtcgtacttc acttccgtac ccagcaccgt ctcggagccg gaggattcaa atccctccgg
     2341 cggcgtctgc tcctgctcac cggcccggaa taccaccgtg acgccggata tattggtatt
     2401 cccctcactg tccagcaccg gcgtaccgtt cagcagcacg ctttttaatc catccaccgg
     2461 tccttgtatc ggcccttcgc tgatgacatc gatcacactc agcaactgcg tggacttcag
     2521 gttgtccttc gcttcgcgcg gggtatgccc cttactgctg cct
//
LOCUS       DBBGLQ010000275         2510 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.276, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000275 DBBGLQ010000000
VERSION     DBBGLQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.276"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            3..365
                     /locus_tag="ACIYSB_005506"
     CDS             3..365
                     /locus_tag="ACIYSB_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432302.1"
                     /translation="MTAVAMWQSYRLGRAGEARDAAEQQLGEYSRALRESSAVISDAS
                     AVLHEVMTQRQQRAQEGEVRREQLREITATDECARALPDHRFTDRLREHTERATARAL
                     LPVHSGGTDDGNTAPALP"
     gene            497..961
                     /locus_tag="ACIYSB_005507"
     CDS             497..961
                     /locus_tag="ACIYSB_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432303.1"
                     /translation="MLKSKSLSTHMSTACRWCRANPEKFTVFIERGGIETTGETPTFV
                     YNYRLVMFVMDYTGDLDDLTLPLIVWLAENQPQLLLNPERNGDIQFTAAINDDDSADV
                     LFEVPLRERVRVTRRDDGTLTTEHLPEPKSRIPQDGDWSHILEDVTWEVTGD"
     gene            954..1595
                     /locus_tag="ACIYSB_005508"
     CDS             954..1595
                     /locus_tag="ACIYSB_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGC5432304.1"
                     /translation="MTDRAFTELDRTMRTISSGLSPQALSRIAGQLARELRRSQQQRM
                     RAQKAPDGTQWEPRRRRISRLQQRIRFIRNNETRTLKNWHHDVSRHGRTITGWDEDKG
                     GLRTFYREDITRYLEISTRRISRNATKTVPMFAKLRTARYLKARGDAGGLTVGYDGVA
                     ARIARVHQFGERDRVAAGVYTDYPARELLGITPADERLIADRLLNRIAGEISS"
     gene            1592..2173
                     /locus_tag="ACIYSB_005509"
     CDS             1592..2173
                     /locus_tag="ACIYSB_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGC5432305.1"
                     /translation="MNAELMRLISNIIRTGVIFDVNPQTWEVRVRSGGLETGWLRWST
                     ARAGAFSVWVPPATGEQVAFVCIGGNPETAIIIGSLWSDDIPAPGSTLKEIIMTAPDG
                     AVFRYDADAGALEVKGIKTASIQADTRITLDTPEVVCTRKLITATLEVQNGGEMRGSI
                     SHSGGSLTSNGITVHTHTHGGVRTGPGTTGGPQ"
     gene            2170..>2510
                     /locus_tag="ACIYSB_005510"
     CDS             2170..>2510
                     /locus_tag="ACIYSB_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGC5432306.1"
                     /translation="MTAVYRGMNPQGTGTLSDSEHLKNSVRDILTTPPGSRVMRREYG
                     SLLPDLIDEPMNDTTRLQCMSATVTALARHEPRIALQNVDVNWRAGGRAVVTLSGIIT
                     ETMQNITFSIT"
BASE COUNT          568 a          764 c          784 g          394 t
ORIGIN      
        1 ccctgacggc agtggccatg tggcagtcat accggctggg gcgtgccggt gaggcacgtg
       61 acgccgcaga gcagcagctg ggcgaataca gccgtgcgct gcgtgaatcc tccgccgtta
      121 tcagcgatgc ctccgccgta ctgcatgagg tgatgacgca gcgacagcaa agggcacagg
      181 aaggagaagt cagacgtgaa cagcttcgcg aaattactgc gacggatgag tgcgcccgcg
      241 ctcttcctga ccaccgtttt actgaccggc tgcgcgaaca cacggaacgc gccactgccc
      301 gcgccctcct tccggttcat tccggcggga ctgacgacgg gaacacggcc cccgcccttc
      361 cctgaacacc ccacctgggg aaatatcggg atatggagcg acagcctcct ggatgcactg
      421 gaaacctgca acgcagacaa acaggccatt gaggcgctgg agcgccggag actcaaccga
      481 cagaacggag ggggacatgc tgaaaagtaa atccctgagc acacacatga gtacagcgtg
      541 ccggtggtgc agggccaacc cggaaaagtt caccgtgttc attgaacgtg gcgggataga
      601 gaccaccggc gagacgccga cgttcgtgta caactaccgg ctcgtgatgt tcgtcatgga
      661 ctacacgggc gacctggacg acctgacgct gccgctgatt gtctggctgg cagagaacca
      721 gccgcagttg ctgcttaatc ctgagcgcaa cggcgatatt cagttcacgg cggccattaa
      781 cgacgacgac agtgcggacg tgctttttga ggtgccgctg cgcgagcgcg tgcgcgtcac
      841 ccgcagggat gacggcaccc tcacaacgga acatctgccg gagccgaaat cgcgcatccc
      901 gcaggacggc gactggtcgc acattctgga ggatgtgacg tgggaggtga ccggtgactg
      961 acagggcttt cactgaactg gaccgcacaa tgcggaccat cagcagcgga ctttccccgc
     1021 aggcactgag ccgcattgcc ggacagcttg ccagagagct tcgccgcagc cagcaacagc
     1081 gtatgcgcgc acagaaagcg ccggacggta cacagtggga gccgcgcaga cggcgcatca
     1141 gccgcctgca gcagcgtatc cgcttcatcc ggaacaacga aacccgcacg ctgaaaaact
     1201 ggcatcacga cgtgagcaga cacggacgca ccatcaccgg ctgggatgag gataagggag
     1261 gcctgcgcac cttctaccgg gaggatatca cccgttatct tgaaatcagc acccgccgca
     1321 tcagccgcaa cgccacaaag accgtaccga tgtttgcgaa gctgcgcacg gcacgctacc
     1381 tgaaagccag aggcgatgcg ggcggtctga ccgtcggtta tgacggcgtg gcggcacgca
     1441 ttgcccgcgt tcaccagttc ggggagcgcg accgggtggc cgcaggggtg tatacggatt
     1501 atccggcccg tgaacttctt ggaataacac cggcagatga acggctgatt gccgacaggc
     1561 tactgaaccg cattgcggga gaaatatcat catgaacgcg gagcttatgc gccttatcag
     1621 caacattatc agaaccggcg tgattttcga cgtgaacccg cagacctggg aggtccgggt
     1681 gcgcagtggc ggactggaga cagggtggct tcgctggagc accgcgcggg cgggcgcgtt
     1741 cagtgtctgg gtgccgccag ccaccggtga acaggtggcg ttcgtctgta tcggtggtaa
     1801 tcccgaaacc gcgataatca tcggcagcct ctggagcgat gatatcccgg caccgggcag
     1861 cacactgaag gaaatcatca tgacagcacc ggacggtgct gtattccgct acgatgcaga
     1921 cgccggtgca ctggaggtta aggggattaa aacagccagc attcaggccg acacgcgcat
     1981 caccctggac acgccggagg tggtgtgtac ccgcaaactt atcaccgcca ccctggaggt
     2041 gcagaacggc ggcgagatgc gcggcagcat atcccacagc ggcggcagcc tgacatccaa
     2101 cggtatcacc gttcatacgc acacccacgg tggcgtcagg acgggcccgg gcaccacggg
     2161 aggcccgcaa tgaccgctgt ttacaggggg atgaacccgc agggaaccgg cacgctgagc
     2221 gacagcgaac acctgaaaaa cagcgtgcgg gatatcctga ccacaccgcc aggcagccgg
     2281 gtgatgcgcc gcgaatacgg cagcctgtta ccggacctca tcgacgagcc catgaacgac
     2341 accacgcgtt tacagtgcat gagcgccacg gtgactgcgc tggcgcgcca tgaaccccgt
     2401 atagcactgc agaacgtcga tgtgaactgg cgggctggcg gacgcgccgt ggtgacgtta
     2461 tccggcatca tcaccgaaac catgcagaac atcacattca gtatcaccat
//
LOCUS       DBBGLQ010000276         2501 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.277, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000276 DBBGLQ010000000
VERSION     DBBGLQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.277"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            157..300
                     /locus_tag="ACIYSB_005511"
     CDS             157..300
                     /locus_tag="ACIYSB_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432307.1"
                     /translation="MGQLAGHRMRILRIFSVAKMGQNAAKGAKMGQKKSGLSWLIVVA
                     DNV"
     gene            complement(584..1018)
                     /gene="uspF"
                     /locus_tag="ACIYSB_005512"
     CDS             complement(584..1018)
                     /gene="uspF"
                     /locus_tag="ACIYSB_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGC5432308.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1159..2292)
                     /gene="ompN"
                     /locus_tag="ACIYSB_005513"
     CDS             complement(1159..2292)
                     /gene="ompN"
                     /locus_tag="ACIYSB_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HGC5432309.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          714 a          523 c          564 g          700 t
ORIGIN      
        1 acgttatgca tggattcgaa ttgctcataa ttcaccagca cagctttaaa tagtaaagag
       61 aaccacgcta cgctgtatgc gagaatgaat tgtggttggc tggtgaaatt ccgttagtgg
      121 atttacagcc ggaactgatt ctctaggaaa gaaatcatgg gccaactcgc aggccacaga
      181 atgagaattc ttagaatttt ttcggtggca aaaatggggc aaaacgctgc aaaaggggca
      241 aaaatggggc aaaaaaagag tgggttatcg tggcttattg tagttgccga taatgtttaa
      301 catattgaaa aataaataaa atacttatgg gtcagatagt tgtgattttt gcccttactt
      361 gttcaggttg tattgttctt tcttactaat ttcttgattt tgcgacattt aaaagcgact
      421 caattcgtta tatggcatca gaagagtatg cgtcatgccg gaatgcccag cataagaaat
      481 ctgaaataaa aacagtggtg tgtatggtac ggattagagg ggaaaatgtc agcacatttg
      541 cgaaatgaat caaaaagccc gcagcaatgt gcgggcgtta gtgtcagcgc acaaccagca
      601 cggagcactc tgcgtgacgc actacagcag cggcgttgga accgagcaga taagtggtga
      661 tatccggtcg atgggaagca atgatgatca tatgagcggg gatcttcttc gccaattcca
      721 gaatgcggtc tttgggcgag ccttcctcaa catggacatg cactctgtcg gttggcagtt
      781 taaatttttt aatgatctct tccagttgcg atttggcttc cgctttcagg tcatccattg
      841 ccggtaattc tgcggaatac gctaaaccca gagaggcata gtagggcagt gaaggtatta
      901 ccgtcaggaa atgaacctct gcatcatcaa tctttgcctc tgcctcaacg tggctaatca
      961 cgcgttgagt taattctgaa tcggaaatat cgatagggac aagaatcgtt ctgttcataa
     1021 aacctcctgt tttagtatcc gcataaagtg taacgccaga tgacactttt tgtgtaatga
     1081 cggagttcac atttttaatt tagatcaaag gaggaagaat aagcagaaaa agcccgccat
     1141 aacagcgggc aggaggattt agaactgata aaccagacct aaagcgacaa tatcatcggt
     1201 agagatgccg ttggcagcgt agaaactgtc atcttcatcc aacaggttga ttttatagtc
     1261 aacataagtg gacatgtttt tattgaaata gtaagtcgcg ccaacatcgg cgtatttaac
     1321 cagatcttta tcatcaacac ctgccgggtt gtctgcacca ccggcagcgt gcaggtcacg
     1381 gcctttagac atcaggaaag agactgccgg acgcagacca aaatcaaact ggtactgtgc
     1441 agtgacttca aagttctggg ttttgtttgc aacagcataa tcgctgtcgc caaacggggt
     1501 catattacgc gtttctgaat acatggttgc caggtaaata ttgttagcat cgtattttag
     1561 cccagcagtc cacgcgtctg ctttatcacc acccgccgca gtatggttaa cctggtcatt
     1621 ggtgcggtca gaagaggtgt atgccgcacc agcgctaaag cccatgccta aatcatatgt
     1681 tgtggaaaga ccccagccgt caccgttttc atgacgaaca tcacgtccgt tgttggtgcc
     1741 ttcctgacca ttactggctc cttcgttgtt accttgatac tgcaccgcga agttcagacc
     1801 atttaccaga ccgaagaaat cagtattacg ataagtcgcg acgccattgg ctcgaccagt
     1861 cataaagttg tctgcattgg tataagagtc accgccaaat tcaggcagca tatcggtcca
     1921 gccttcgatg tcgtacatta cgccataatt acgtccgtaa tcgaaagaac cgtaatctgc
     1981 aaatttcagt ccggcaaatg ccagacgggt ccatgactgg ttttttgaag attcagtgtt
     2041 gtttgcctga atattgtatt cccattgacc gtagccagtg agttgatcgt taatttgggt
     2101 ttcgccttta aaacccagac gcgcatagct ctggtcgcca tctttcgctg aattatcaga
     2161 aaaataatgc aggccatcaa ctttgccata cagatctaat ttgttgccgt ctttattata
     2221 aacttcggct gcatgtgcag cacctgcggc gagcagggca ggaattaaaa gtgccagtac
     2281 tttgcttttc attgaataaa tcctttagtt attttatttg ccttttatcc ccatttcggg
     2341 gagtgaaaac atcctaaagg agaagttcag ttagccgata aatatcattt gttacgcctt
     2401 aagtaaaacc ttaatcaaat aattcttctg aaatgataat aagtctggtg aatgtatcga
     2461 agataataca tacaaaataa aaattatact tttaattttc t
//
LOCUS       DBBGLQ010000277         2421 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.278, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000277 DBBGLQ010000000
VERSION     DBBGLQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.278"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..1960
                     /gene="recE"
                     /locus_tag="ACIYSB_005514"
                     /pseudo
     CDS             1..1960
                     /gene="recE"
                     /locus_tag="ACIYSB_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            1953..>2421
                     /locus_tag="ACIYSB_005515"
     CDS             1953..>2421
                     /locus_tag="ACIYSB_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HGC5432310.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDE"
BASE COUNT          706 a          587 c          607 g          521 t
ORIGIN      
        1 accgaacaca ttgatcgtgg tcttctgaca aaagaatgga ggaaagggaa tcgtgtttca
       61 cgcatcactc gcacggcttc cggtgctaat gctggcggcg ggaacctcac cgatcgcggc
      121 gaaggtttcg tccacgatct gacgtcactg gcacgcgatg tagccactgg cgtactggcc
      181 cgttcaatgg acgtggacat ctataacctt catccggcac acgctaaacg cattgaggaa
      241 attatcgctg aaaataaaac cgcccttttc tgttttccgc gacaaattca tcaccatgcc
      301 tggcgggctg gattattccc gcgccatcgt ggttgcgtcc gtgaaagaag caccaattgg
      361 gatcgagctc atccccgcgc acgtcactga atatctgaac aaagtactga ctgaaactga
      421 tcatgccaac cctgatccgg aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat
      481 gccgcagcgt gtgacagaag aaggaaaaca ggacgatgaa gaaaaaccac aaccatctgg
      541 cggaatggca gatgaacagg caacggctga aacagtggaa ccgaatgcaa ctgaacatca
      601 tcagaacacg cagccgctgg atgctcagtc acaggtaaac cctgttgaag cggaatacca
      661 gaaaaagctg gcagaactcc atgaagcccg gaaaaacatt ccatcaaaaa atcctgtcga
      721 tgccgataaa ttgcttgctg catcacgtgg tgaatttgtt gacggaatta gcgacccgaa
      781 cgatccgaaa tgggttaagg ggatccagac tcgcgattct gtgtaccaga accagccaga
      841 aacggaaaaa accagcccgg atgtgaaaca acctgagcca gtagtgcaac aggaaccgga
      901 aatagtctgc aatgcctgcg gtcagactgg cggggataac tgccctgact gtggtgcggt
      961 gatgggcgac gcaacatacc aggaaacatt cgatgaagag aatcaggttg aagctaagga
     1021 aaatgatccg gtggaaatgg aaggcgctga acatccgcac aatgagaatg ctggcagcga
     1081 tccgcatcgc gattgcagtg atgaaactgg cgaagtcgca gatcccgtaa tcgtagaaga
     1141 catagagcca ggtatttatt acggaatttc gaatgagaat taccacgcgg gtcccggtgt
     1201 cagtaagtct cagctcgatg acattgctga tactccggca ctgtatttgt ggcgtaaaaa
     1261 tgcccccgtg gacaccacaa agacaaaaac gctcgattta ggaaccgctt tccactgccg
     1321 ggtacttgaa ccggaagaat tcagtaaccg ctttatcata gcacctgaat ttaaccgccg
     1381 tacaaacgcc ggaaaagaag aagagaaagc gtttcttatg aaatgcgcaa gcacaggaaa
     1441 aacggttatc actgcggaag aaagccggaa aattgagctc atgtatcaaa gcgttatggc
     1501 tttgccgctg gggcaatggc ttgttgaaag cgccggacac gctgaatcat caatttactg
     1561 ggaagatcct gaaacaggaa ttttgtgtcg gtgccgtccg gacaaaatta tccctgaatt
     1621 tcactggatc atggacgtga aaactacggc ggatattcaa cgattcaaaa ccgcttatta
     1681 cgactaccgc tatcacgttc aggatgcatt ctacagtgac ggttatgaag cacagtttgg
     1741 agtgcagcca actttcgttt ttctggtcgc cagcacaact attgaatgcg gacgttatcc
     1801 ggttgaaatt ttcatgatgg gcgaagaagc aaaactggca ggtcagctgg aatatcaccg
     1861 caatctgcga accctggctg actgcctcaa taccgatgaa tggccagcta ttaagacgtt
     1921 atcactgccc cgctgggcta aggaatatgc aaatgactaa gcaaccacca atcgcaaaag
     1981 ccgatctgca aaaaactcag ggaaaccgtg caccagcagc agttaaaaat aacgacgtga
     2041 ttagttttat taaccagcca tcaatgaaag agcaactggc agcagctctt ccacgccata
     2101 tgacggctga acgtatgatc cgtatcgcca ccacagaaat tcgtaaagtt ccagcgttag
     2161 gaaactgtga cactatgagt tttgtcagtg caatcgtaca gtgttcacag ctcggccttg
     2221 agccaggtag cgcccttggc cacgcatatt tactgccttt tggtaataaa aacgaaaaga
     2281 gcggtaaaaa gaacgttcag ctaatcattg gctatcgcgg catgattgat ctggctcgcc
     2341 gttctggtca aatcgccagc ctgtcagccc gtgttgtccg tgaaggtgac gagtttagct
     2401 tcgaatttgg ccttgatgaa a
//
LOCUS       DBBGLQ010000278         2384 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.279, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000278 DBBGLQ010000000
VERSION     DBBGLQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.279"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>2384
                     /gene="rhsB"
                     /locus_tag="ACIYSB_005516"
     CDS             <1..>2384
                     /gene="rhsB"
                     /locus_tag="ACIYSB_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HGC5432311.1"
                     /translation="AVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSF
                     TYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLD
                     RREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGL
                     ITRITTPDGRASAFYYNHHNQLTSATGPDGLELRREYDELGRLIQETAPDGDITRYRY
                     DNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEG
                     LSQYRAYDSRGQLIAVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRT
                     TQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGK
                     LIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVAVHY
                     RYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSG
                     YLAGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYT
                     WNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELH
                     PDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHR
                     LVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGD
                     RLTTIQNDRSRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDG"
BASE COUNT          562 a          726 c          734 g          362 t
ORIGIN      
        1 ctgccgtgtg gctgacgcac gacccggaat acccggagaa tttacctgcc gcgccgctgg
       61 tgcgctatgg ctggacgcca cgcggcgaac tggcggtggt gtatgaccgt agtggcaaac
      121 aggtgcgcag ctttacttac gatgataaat accggggccg gatggtggcg caccgtcaca
      181 cgggccggcc ggaaatccgt taccgttacg acagcgacgg gcgggtgaca gaacagctaa
      241 acccggcagg cttaagctac acgtatcagt atgagaaaga ccgcatcacc atcaccgaca
      301 gcctggaccg ccgtgaagtg ctgcacacgc agggcgaagc cgggctgaag cgggtggtga
      361 aaaaggaaca cgcggacggc agcgtcacgc agagtcagtt tgacgccgtg ggcaggctca
      421 gggcacagac ggatgccgca ggcaggacaa cagagtacag cccggatgtg gtgacgggcc
      481 tcatcacgcg cataaccacg ccggatggca gggcatcggc gttttactat aaccaccaca
      541 accagttaac gtcagccacc gggcctgacg ggctggaatt gcgccgggaa tatgatgaat
      601 tgggccgtct gattcaggaa actgcccctg acggcgatat cacccgctac cgttatgata
      661 atccacacag tgacttaccc tgcgcaacgg aagatgccac cggcagccgg aaaaccatga
      721 cgtggagccg ttacggtcag ttgctgagct tcaccgactg ttccggttat gtaacccgtt
      781 atgaccatga ccgcttcggg cagatgacgg cggtgcaccg cgaggaaggg ctgagtcagt
      841 accgcgcata cgacagccgt ggacagttaa ttgccgtgaa agacacgcag ggccatgaaa
      901 cgcggtatga atacaacatc gccggtgacc tgaccgccgt cattgccccg gacggcagca
      961 gaaacgggac acagtacgat gcgtggggaa aggccgtccg taccacgcag ggcgggctga
     1021 cgcgcagtat ggaatacgat gctgccggac gggtcatccg cctgaccagt gaaaacggca
     1081 gccacaccac cttccgttac gatgtacttg accggctgat acaggaaacc ggctttgacg
     1141 gccgcacaca gcgttatcac cacgacctga ccggcaaact tatccgcagc gaggatgagg
     1201 gtctggtcac ccactggcac tatgacgaag cagaccgcct cacgcaccgc accgtgaagg
     1261 gtgaaaccgc agagcggtgg cagtatgacg aacgtggctg gctgacagac atcagccata
     1321 tcagcgaagg gcaccgggtg gcggtgcatt acaggtatga tgagaaaggc cggctgaccg
     1381 gtgagcgtca gacggtgcat cacccgcaga cggaagcact gctctggcag catgagacca
     1441 gacatgcgta caacgcgcag gggctggcga accgctgtat accggacagc ctgcccgccg
     1501 tggaatggct gacctacggc agcggttacc tggcaggcat gaaactcggc gacacaccgc
     1561 tggtggagta cacccgcgac cgcctgcacc gggaaacgct gcgcagcttc ggccgttatg
     1621 aactcaccac cgcttatacc cctgccgggc agttacagag ccagcacctg aacagcctgc
     1681 tgtctgaccg cgattacacc tggaacgaca acggcgaact catccgcatc agcagcccgc
     1741 gccagacccg gagttacagc tacagcacca ccggcaggct gaccggcgtt cacaccaccg
     1801 cagcgaatct ggatatccgc atcccgtatg ccacagaccc ggcaggtaac cgcctgcccg
     1861 acccggagct gcacccggac agcaccctca gcatgtggcc ggataaccgt atcgcccgtg
     1921 acgcgcacta tctttaccgg tatgaccgtc acggcaggct gacagagaaa accgacctca
     1981 tcccggaagg ggttatccgc acggatgatg agcggactca ccggtaccat tacgacagtc
     2041 agcaccggct ggtgcactac acgcggacac aatatgaaga gccgctggtc gaaagtcgct
     2101 atctttacga cccgctgggc cgcagggtgg caaaacgggt gtggcggcgt gaacgggacc
     2161 tgacgggctg gatgtcgctg tcacggaaac cgcaagtgac ctggtacggc tgggacggcg
     2221 accggctgac cacgatacag aacgacagga gccgcatcca gacgatttat cagccgggga
     2281 gcttcacgcc actcatcagg gtcgaaactg ccaccggtga gctggcgaaa acgcagcgcc
     2341 gcagcctggc ggatgccctt cagcagtccg gcggcgaaga cggt
//
LOCUS       DBBGLQ010000279         2337 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.281, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000279 DBBGLQ010000000
VERSION     DBBGLQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.281"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(139..552)
                     /locus_tag="ACIYSB_005517"
     CDS             complement(139..552)
                     /locus_tag="ACIYSB_005517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432312.1"
                     /translation="MGAKFLDQKTGVILEEPEFVKLYIRDLCRVKGLSATQYKIFTFM
                     LANMNTDNVVSFGPRTKEKFLSEHSIKPQTFNNNVSQLIESCLISRIARNEFLVNKKY
                     AVKVDWAKVQKIVWTTTYSNEGIDSTVTIAPKKTD"
     gene            1961..>2337
                     /gene="mobA"
                     /locus_tag="ACIYSB_005518"
     CDS             1961..>2337
                     /gene="mobA"
                     /locus_tag="ACIYSB_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HGC5432313.1"
                     /translation="MTKNKRSGSEVRQRTKVITLRVNNRIANEIRRRAKSEGLTISEY
                     IRTTSLNNEIKQRVPSRYLDELIQLGRMQKKLFDKGKRPKDKEYLDVMHKIISLCAEM
                     KEVTQRISDIYKKMDLIKEEIKA"
BASE COUNT          697 a          486 c          487 g          667 t
ORIGIN      
        1 aaaaaaaata cataaaaata aatacccagg ttccaactta tttaattgag cagaacatag
       61 gtcttcataa aaatttaaag acctataata gaattagcga cattttgttt tatcgaaatc
      121 ttttacaatc ataagaaatt aatccgtttt tttcggggct atcgtcactg ttgaatcaat
      181 cccttcatta ctgtaagttg ttgtccatac tatcttttgt actttcgccc aatcaacttt
      241 aactgcgtat tttttattaa ccaaaaattc atttctggct attctagaaa ttaaacatga
      301 ctcaatcagt tgagacacat tattattaaa tgtttgtggt ttaattgaat gttctgataa
      361 aaacttttct ttggttcttg gtccaaatga aacaacatta tcagtattca tattagcaag
      421 catgaacgta aaaattttgt attgcgttgc tgatagccct ttaacacggc ataaatcacg
      481 gatatacagc ttcacgaact ccggctcttc caaaattacg ccagtttttt gatctaaaaa
      541 tttcgctccc attctcacct cgcaggtaat ttagttaccg ttttatcggt atcttatgta
      601 tgttctacaa ccgagtaaat actttttttc cggtatttga ttagcaacaa aaacagtatt
      661 ccggtatttg aaataccgct gagcggtatt tcaacctccg ctcagcggta aaaactttcc
      721 cgctgagcgg tatatctatt cccgctgagc ggtatttagt ttgcctctag gccttgtcac
      781 acctgatttt cctatctgtc tgtgaatcta tcttagatct cgtgcgtaag tgcactatgc
      841 caatactggt tcccttccgc ttcctcgccc actcgcgagc ctagcggctc ggtcgctggg
      901 gctgcggcga gcggtgccac ctctcgcata tcgttgacga tcccgatttg atctcaggcg
      961 atcgagtcgc tggccaccgg ttaaacgcgc tcaaagcctc ccagacgcac aaaacggggg
     1021 ttaaatgccc gtcagggcat ggaaggcgcg aaaaacgctt acagaggttt tcagaaggtt
     1081 aggcgcgccg atacccctaa ccaggggctt tcaggtcgct aggaaccgcg ccagccccct
     1141 gcaaggccca tttgcacgga acagacagcc actcggttgg taggtggtcg tagaccgtcg
     1201 gtgttacagc gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg
     1261 ctgccggaac cgtggcggct tcgatggtga agggtgagcg cgtttccaag aggttttcca
     1321 ggcaagctat gactgaactg aacagattga acgaggagga aacgcgctca gacgctttgt
     1381 agcgcgcttt tctgtgtcag gcaagtttgt tgtgtctttt gcctttcagg ttgttagcag
     1441 ctggcggagc actgcacggg gattaatgct cccattgcta ggatgaaata aagattagga
     1501 gcgaagttaa gttatatttt taatatataa acgcgatatc aataactcac atagttatat
     1561 tttatgcata tgctaccatc ttgctctttt gcctcctgct ctctggacga atcccgcatt
     1621 accaacaggc aatggattat ataaccaaga agatctaatc tttattcctc taataatcca
     1681 caaacgacag acaacaaaga catcaattaa ttcggtgtta tttattaaat aacaagcaat
     1741 aatttttaaa atctgtgtaa ataatattga atgaagcatc atttttttgt atcagaaata
     1801 ccaggttacg acgcggtatt ttattttcgt cgaaaagttg cagcggaaaa tgggacagga
     1861 tgtgtattcg tcataccgcg aatacattcg cttgcatgag aatacctcct gtcagggcaa
     1921 gccccgacac cccaaacgtg caaaatggag aatcattgcg atgactaaaa ataagcgtag
     1981 cggttcagaa gtacggcaga ggactaaggt tatcaccctt agagtaaata acagaatcgc
     2041 aaacgaaatc agacggcgag cgaagagcga agggctgact atttcagaat atataaggac
     2101 aacaagctta aataatgaaa taaaacagcg cgttccttcc agatatttag atgaattaat
     2161 ccaacttggt cggatgcaga agaaattatt tgataaaggg aagagaccga aggacaaaga
     2221 gtatctggat gtgatgcata aaataatttc actttgtgct gaaatgaaag aagtaacaca
     2281 gcgaatttct gatatatata aaaaaatgga tttaataaaa gaagaaataa aagcaat
//
LOCUS       DBBGLQ010000280         2333 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.282, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000280 DBBGLQ010000000
VERSION     DBBGLQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.282"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(634..879)
                     /locus_tag="ACIYSB_005519"
                     /pseudo
     CDS             complement(634..879)
                     /locus_tag="ACIYSB_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(941..2176)
                     /locus_tag="ACIYSB_005520"
                     /pseudo
     CDS             complement(941..2176)
                     /locus_tag="ACIYSB_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          580 a          583 c          483 g          687 t
ORIGIN      
        1 aataaagtat actgaccaga aattgtaaaa ttcgtatatt cttattcata ctgttttttc
       61 tttagtgatg cattttttat caaccataca agcacattat ttcattgata ctaatcacat
      121 caggatgaat gttttttctc aatagaaata agtgtcttta ttccttacca atttattctg
      181 gttattcctc ttatccagac gtctcgtgat actccctgta tttattatag agtatatata
      241 tgtactgcgt ttaaatcatg aagacgacaa cctgttttac ataaattatt cctaagtatt
      301 tttccagaga agacctaggt ataatgatgt gcactgtctg gttttcttac aactgactta
      361 gcgatctgca tatccgtgat aacgcctgaa taaataggac gagagaactc ttaaccaaat
      421 aatctaacac ttttaatcga caggaattaa aaacccgggg aggttaaaaa aacacaaaaa
      481 aatggatgtt tattcaactc tggttacgta ccatgaggac tgcatccaca atgcaccggc
      541 aattaaatgg ctttcattga ttgatttttt atatctgtga tgattttttg ctccattcct
      601 caaaaaatgg acgcaacttc ccctctgcgg ctctcagctg tatacccgct gtcgtgccag
      661 tggctcatgc atcatgacca gctctgccag acggtagccc cgcttcagcc ccgtaaaccg
      721 catctgacct ccgcacagga tgcacttcag cgggtcaacc ttcagtaacc gctgatacac
      781 ccttctccgc ctgatttgca tcgccgtttt tctctccgtt tctgttatga cgtacacaac
      841 attttccagt aaccggcgct tcaccggact caggaacccg ttgtcaacgc cacgatgttt
      901 gaccgttctg gggatgcgga cgatttcctg gactgcttta tcattccagc ccgcattgtt
      961 ggcggtacat tttggggctg actgcacccg gcgataattt tatacgccgc tcgttatacc
     1021 atctgatgta agtatccact tgctgcatga acttttctgg cgtgatgccc gaccatttac
     1081 gcccatgata catttccgtt ttcagtctgc cgaaaaagcc ttcgcatgcc gcattatcag
     1141 gtgaacatcc tttgcgggac attgagcgaa taagaccttc tgcattcact ctttccagcc
     1201 agcctgacca gcgataatgc ccacctctgt cactgtgtat caccggtcgt tcgccagcat
     1261 ttaacgtttc gacagcgcta tccagcatag tgttgaccag ttcagcatcg gggcgtgtac
     1321 tgagagacca gctcacaact tttccatcga agcagtccac caccgatgat agccagactt
     1381 tacctgcagg gaggtggaac tccgtgatat ctgtcagcca tttctgatta ggttgctccg
     1441 ccttaaaatc tctggcgata aggttatccg gagccggtcc gatttctccg caatatgagc
     1501 tgtagcggcg acgacggtta cggctgacga caagctgttc ttccaccata agtctgcgta
     1561 caaccttttc tgatagccgc cccccctcat gcctgagcat cgcatgcagg cgacgatagc
     1621 cataacactg gtaattactg ttaaatatat ctgtcagcat cgtacgtatc gtggcgtatt
     1681 tatcaccggc tttcagtgca gcccggtgat aaaaataact gctacgggca aggcccagaa
     1741 catgcagtaa ttctgtcagg ggatatgttt gtctcagggc atcagcgatc ttcgttttct
     1801 ctctgttgtt caggtgactg atactgatgc ccgggtcttt ttttatgatt tcctccgcct
     1861 ttttcagaat atccagctcc atctgccggc ggcgtatttc ctgattcagt cgggcgactt
     1921 cctcccgcaa cgcatcgcgt tctgcctcca gggaaggttc gttatgttta cgcatagtct
     1981 ggtaagcact gttgccgata attttatctt tccatttata caataccgca cgactgacac
     2041 caatacgcct ggcgatttca ctggctgata cttgtcgtgt acagagtgcc atgacagcct
     2101 gacgtttgac ttctggttca aagggcgcaa ccgggttggc cttactggta aatatacgtc
     2161 gtctgtcggg ataaaggtaa gcgtaaactg accgccgtat gtagccatca gacgagaatt
     2221 ggtaacttag acgcccatct gatatagacg gacatctaag tatggaatta caggactggc
     2281 gaaaagaacc tcgtaaaaac tattcgaatg aattcaaact tcgtatggtg gaa
//
LOCUS       DBBGLQ010000281         2300 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.283, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000281 DBBGLQ010000000
VERSION     DBBGLQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.283"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>2300
                     /locus_tag="ACIYSB_005521"
     CDS             <1..>2300
                     /locus_tag="ACIYSB_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5432314.1"
                     /translation="LSGSDNTIQQAIRRTQQYNDQLERERQALARVTQARQQYSRAQE
                     TAGKLKTVGLTAVGTAAAGGYAAGRFLQPAVSFGKEMSRVQALTRIDKNSPQFKALRE
                     QAIKLGSETQFTAGDAASGQAFLAMASFTPQAIQAALPGVLDLAMAAGMDLGQTADIS
                     SNILTQFGLSADQMDRVGDTLAATFTRTNTDLRSLGETMKYTGPVAASLGLSLEQAAA
                     MTGVLGSVGIRGSDAGTALRASLSRLVDPPKAAAMALKELGVETQDAYGNMRRMENIL
                     NELYQATRKYGNAAQVSFFRAIAGQESYVSLMNLVTAAGENNLPRLTQEIELATGELN
                     QTARVMADNLDGDLKSLNSAWEGLRIRVADLVDGPLRSVTQSFTRVIAKVTALAQAHP
                     ELTKQLLIAGGVLLAAVAAMGALSLATGVLMGPLAKLRLGFALLTGTSGLGRALPLLT
                     QLRGVLGGPLGGIGGWRVLFVGITSGAGRFAALLGVVAGRLRGVQAGLGALRGGLLAA
                     FTSPGGALISLGRGIGILALRLSGLPALWGMVSGAVGALGGALGFLLSPVGLVVATLV
                     GAAVLIWRHWDQLKAVVGGFLSGLWQGLTPLREAVAPLGPLFEYVGQTIGRVWQWFVD
                     LFAPVSTTKDELDKCTEAGRTFGQKVGEFISNLVTGPVTLLIDSLTRVLEKLGLIPSA
                     VERARKKAEELKQKELLNEKAAMLLADVNVINPPKKDEDKKYGEKPKGGQPPLTGENT
                     GTMRRLNKIVDNTGGLLDEAKQAKKR"
BASE COUNT          453 a          604 c          769 g          474 t
ORIGIN      
        1 cgctttccgg cagtgacaac accattcaac aggccatacg gcgaacacag caatacaacg
       61 accagctgga acgcgaacgg caggcgctgg cacgtgtcac gcaggcacgc cagcaatatt
      121 cccgtgctca ggagacggcg ggaaaactta aaacggtcgg acttacggcg gttggtacgg
      181 cggcggcagg cggctatgcg gccggacgtt tcctgcaacc ggcggtcagt tttggtaagg
      241 agatgtcgcg cgtccaggcg ctgacccgta ttgacaaaaa cagtccgcag tttaaggcgc
      301 tgcgcgaaca ggccattaag ctgggctctg aaacgcagtt taccgccggt gatgctgcca
      361 gcggtcaggc gttccttgcc atggccagct tcacaccaca ggccatacag gcggcgctac
      421 ccggcgtgct tgatctggca atggctgcag ggatggacct gggacagact gccgatatca
      481 gttcgaatat cctcacacag tttggtctgt ccgctgacca gatggatcgc gtgggtgaca
      541 cgctggccgc caccttcaca cgaaccaata cggatctccg ttcgctgggt gaaaccatga
      601 agtacacggg gccggttgca gcttcacttg gcctgtccct tgaacaggcg gcagccatga
      661 cgggtgtcct tggcagtgtg gggatccgtg gaagtgatgc aggtacggcg ctgcgtgcca
      721 gtctgtcacg tctggttgat ccgccgaagg ccgctgcaat ggcactgaaa gagcttggcg
      781 tggagacgca ggatgcttac gggaacatgc gccggatgga aaatatcctg aatgagctgt
      841 accaggccac ccgaaaatac ggtaatgccg cgcaggtctc ctttttcagg gctattgccg
      901 ggcaggagtc ctatgtttcc ctgatgaatc ttgtcactgc ggcgggggaa aataatcttc
      961 cccgtcttac gcaggagatt gaactggcca ccggggaact taatcagact gcccgtgtta
     1021 tggctgataa cctggacggt gacctgaaat cactgaacag cgcatgggaa ggactgcgta
     1081 tccgtgtcgc tgacctcgtt gacgggccgc tgcgttctgt cacgcagtcg ttcacccggg
     1141 ttattgcgaa agtcaccgcg ctggctcagg cgcaccctga actgacaaaa caactgctga
     1201 ttgccggtgg tgtactgctg gctgcggttg cggccatggg ggcgctgtca cttgccactg
     1261 gtgtgttgat ggggccgctg gcaaaactgc gtcttgggtt tgccctgctg accggcacat
     1321 ccggcctcgg tcgtgcgctg ccgttactga cgcagctgcg cggcgtactc ggtggtccgc
     1381 tgggcggtat cggtggctgg cgcgtgctgt ttgtcggtat cacctccggt gccggacggt
     1441 ttgctgcact gctgggcgta gttgcggggc ggcttcgtgg tgtgcaggcg ggacttggcg
     1501 cgctgcgtgg tggtctgctg gctgcgttca cttcacctgg cggcgcactg atttcactgg
     1561 ggcgcggtat cggaatactg gcgctcagac tgtcaggtct gcccgcactg tgggggatgg
     1621 tgagtggtgc ggtgggcgca cttggcgggg cgctgggttt tctgttaagt ccggtgggcc
     1681 ttgtggtggc gacactggtc ggtgcggcag tgcttatctg gcggcactgg gaccagctga
     1741 aggctgtcgt gggcgggttc ttgtccggcc tgtggcaggg gcttacgcca ctgcgcgagg
     1801 ctgtggcacc actgggacca ttgtttgagt atgtcgggca gacaatcggg cgtgtgtggc
     1861 agtggttcgt ggatttgttc gcgccggtaa gcacaacaaa ggatgagctg gacaagtgca
     1921 cggaagccgg gcggacgttc gggcagaagg tcggggagtt tatcagcaat ctggtgaccg
     1981 gtccggttac gctgctgatt gactcattaa cccgggtgct ggaaaaactc gggctgattc
     2041 cgtcagcggt tgagcgcgcc cggaaaaaag cggaagaact aaaacagaag gagttgctga
     2101 acgagaaagc tgccatgctt ctggcggatg ttaacgtcat taatccgccg aaaaaggacg
     2161 aggataaaaa atacggcgaa aaacccaaag gggggcaacc acccctgacg ggggagaata
     2221 ccggcactat gcgccgcctg aacaaaatcg tcgataacac cggtggtctg ctggatgagg
     2281 cgaaacaggc gaaaaaacgc
//
LOCUS       DBBGLQ010000282         2296 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.284, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000282 DBBGLQ010000000
VERSION     DBBGLQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.284"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..207
                     /gene="tnpB"
                     /locus_tag="ACIYSB_005522"
     CDS             <1..207
                     /gene="tnpB"
                     /locus_tag="ACIYSB_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGC5432315.1"
                     /translation="RRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQ
                     LAMLLDKLDWRQPKTSSRNSLTML"
     gene            238..1851
                     /locus_tag="ACIYSB_005523"
     CDS             238..1851
                     /locus_tag="ACIYSB_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HGC5432316.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGKLDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGCIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAARQQRSKALLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNTAERALRAVCLGKKNYVFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNCVDELLPWNVVLTNK"
     gene            complement(1947..2294)
                     /gene="tnpB"
                     /locus_tag="ACIYSB_005524"
     CDS             complement(1947..2294)
                     /gene="tnpB"
                     /locus_tag="ACIYSB_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGC5432317.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPISGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          576 a          596 c          657 g          467 t
ORIGIN      
        1 cgacggggtg acaccgtcaa aattctttgg gctgatgctg atggtctgtg cctgttcacc
       61 aaacgcctgg aggaaggcca gtttatctgg cctgcggtac gtgacggcaa ggtatccatt
      121 acccgctcgc aactggcaat gctcctcgat aagctggact ggcgtcagcc aaaaacatcc
      181 agccgtaact cactgacaat gttgtaaaaa actcctgacc gcattataaa aacggtcatg
      241 agtcagaaat acctcattcg catcgcagag ctggaaaggt tgctctctga gcaggctgaa
      301 gccctccgtc agaaagacca gcaactgagt ctggttgaag agacggaagc cttcctgcgc
      361 tctgcactga cacgtgccga agaaaagatc gaagaagatg aacgggaaat agaacatctg
      421 cgggctcaga tagaaaaact gcgccggatg ctgttcggta cccgttctga aaaactgcgt
      481 cgtgaagttg aactggctga ggctctgctg aaacaacgtg aacaggacag cgatcgttac
      541 agtgggcggg aagacgatcc tcaggttccc cgccagttgc gacagtcgcg ccatcgtcgt
      601 ccgttaccgg cacaccttcc ccgtgaaata caccgcctgg agccagaaga aagctgttgc
      661 ccggagtgtg gcggtaagct ggattatctg ggggaagtca gcgctgaaca gctggaactg
      721 gtgagcagtg ccctgaaagt gatccgcaca gaacgggtaa aaaaagcctg tacaaaatgt
      781 gactgtattg ttgaagcacc ggcgccgtcc cgcccgatag agcgtggtat cgcgggcccc
      841 ggattacttg cccgcgtgtt aacgggaaaa tactgcgaac atctgccact gtatcgtcag
      901 agtgaaatct ttgcccgcca gggtgtcgaa ctgagccggg ccttactctc caactgggtt
      961 gacgcgtgct gccagttaat gacaccggtg aatgatgccc tgtaccgtta tgtaatgaat
     1021 acccgcaagg ttcacactga tgacacaccg gtaaaggtac tggcaccggg tcagaaaaag
     1081 gcgaaaacag ggtgtatctg gacgtatgtc cgggatgatc gcaatgtggg ttcgtcatct
     1141 cctccagcgg tctggttcgc gtactcgccg aaccggcagg ggaaacaccc ggagcaacac
     1201 cttcgcccct tccggggtat cctgcaggcg gatgcgttca caggttacga caggttgttc
     1261 agtgcagaac gtgaaggtgg tgcactgaca gaagttgcgt gctgggccca tgcccggcga
     1321 aaaatccacg atgtatacat cagcagcaaa agtgcgacgg cagaagaagc cctgaagcga
     1381 atcagtgaac tgtacgccat cgaggatgaa atacggggat taccagagtc agagcgtctt
     1441 gcagccaggc agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag
     1501 aagaatggca cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
     1561 cagtgggacg ccctctgtta ttacagtgat gatggtttgg cagaagcgga caataacaca
     1621 gcggaaagag cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc
     1681 gatcacggcg gcgagcgtgg agcactgctg tacgggctga tcggcacctg ccgtctgaac
     1741 ggtatcgatc cggaagcgta tctgcgccat attctgagcg tactgccgga atggccctcc
     1801 aactgtgttg acgaactcct gccatggaac gtagtactca ccaataaata agcgtcaata
     1861 cggtgctccg ttgacgctta caagagatgt cgttcataag cccgtatttt accgggctta
     1921 ttctgtgaca accaggataa agagatttac agcatggtca gggaggtcag cagccgctta
     1981 ggctgtcgcc agtcgatacc ttccagcagc atcgccagct gcgcctgcgt aaggaacact
     2041 ttgccatcac gggctgacgg ccaggcgaag cgcccacgct ccagccgctt ggtcaggagg
     2101 cacagcccgt caccggtgga ccacagcagt ttaacctgac tgccgctgcg gccccggaaa
     2161 atgaaaacat ggccggatat gggatcgtct ttcagcgccg tctgtacttt cgcagccagg
     2221 ccgttgaagc catttctcat atcggtgata ccggcaacca gccaaatttt ggtcccggaa
     2281 ggtaacggga tcatcg
//
LOCUS       DBBGLQ010000283         2285 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.285, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000283 DBBGLQ010000000
VERSION     DBBGLQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.285"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            134..2182
                     /gene="ireA"
                     /locus_tag="ACIYSB_005525"
     CDS             134..2182
                     /gene="ireA"
                     /locus_tag="ACIYSB_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HGC5432318.1"
                     /translation="MKNKYIIAPGIAVMCSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLIKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQVHTLTPGESLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREIKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSIKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYSNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWHVSANYTFTDSRRKSDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGLNYQILPDTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRVSF
                     "
BASE COUNT          712 a          437 c          534 g          602 t
ORIGIN      
        1 ttggttgtct ggagattcag ggggccagtc tactgaatga gcgaaatgtg atttaccttc
       61 ttttctgttc attgtaaatt ccctctttgc taacgcaaat cattatcatt acgcctttgg
      121 caaagggaaa tttatgaaga acaaatatat cattgctccg ggcattgccg tgatgtgttc
      181 tgcagttata tcatcaggtt atgccagttc tgataaaaaa gaagatacgc ttgttgttac
      241 tgcctccggg ttcactcagc agctcagaaa tgccccggcc agtgtctcag tcattacttc
      301 agaacaactg caaaaaaaac cggtttcaga tctggtcgat gcagtaaaag atgttgaagg
      361 gattagtatc actggtggga atgaaaaacc ggatatcagt atacgtggtc taagtggcga
      421 ttacacgctg attctggtcg atggacgacg tcagagcggt cgggaatcca gaccaaacgg
      481 cagcggcggt tttgaagccg gatttatccc tcctgtggaa gcaattgaac gcattgaagt
      541 gatccgtggc cctatgtctt ccctgtatgg ttctgatgcc atcggagggg tcattaatat
      601 cataaccaaa ccagttaata accaaacatg ggatggcgta cttggacttg gggggattat
      661 tcaggaacat gggaaatttg gtaactcaac cacaaatgac ttctatctgt caggcccatt
      721 gattaaggat aaacttggtc ttcagctata tggaggaatg aactatcgca aggaagatag
      781 tatctctcag ggaacaccgg caaaagataa taagaatata acggcaacgc tccagtttac
      841 tccgactgaa agccagaagt ttgtttttga atatggaaaa aataaccagg tgcatacatt
      901 aacacctggt gagtctctcg atgcctggac tatgcgggga aatcttaaac aaccaaacag
      961 taaaagagaa acgcataatt cacgtagtca ctgggtagca gcatggaatg cccagggcga
     1021 aatactgcat cctgaaattg ctgtttatca ggagaaagtt attcgtgaga ttaaatcagg
     1081 taaaaaagat aaatataatc attgggatct taattacgag tcaagaaaac cggaaataac
     1141 caacacaatc atagatgcaa aagtgacggc atttctgccg gaaaatgtac tgaccatcgg
     1201 aggtcaattt cagcatgcag agctccgtga tgactcagcc acgggtaaaa aaacgacaga
     1261 aacacagtct gtttcaatta aacagaaagc tgtttttata gaaaatgaat atgcagcaac
     1321 ggattctctc gccctgactg gaggactgcg tctcgataat catgaaatct atggcagtta
     1381 ctggaatcca agattgtacg ctgtttataa cctgaccgat aatctcacac tcaaaggggg
     1441 gatcgcaaaa gcatttcggg ctccttcaat tcgtgaggtg agtcctggat ttggaacact
     1501 gacgcagggt ggtgcctcta ttatgtatgg aaacagggac ctgaaaccgg agaccagtgt
     1561 aaccgaagag atcggtatta tttatagtaa tgatagtggt ttttcggcga gcgcgacgct
     1621 gtttaatact gattttaaaa ataagttgac cagttacgat ataggtacaa aagatccagt
     1681 caccgggtta aacactttta tttatgataa tgtaggtgag gcaaatatca gaggggtgga
     1741 gcttgcaact cagattcctg tgtatgataa atggcatgta tctgcaaact atacatttac
     1801 tgactctcgt cgaaaaagtg atgacgaaag tctcaatggc aagtcgctga aaggggaacc
     1861 tctggaaaga actcccagac atgcagccaa tgcaaaactg gaatgggatt acactcagga
     1921 tattacattt tattcatctc tgaattatac gggaaaacaa atctgggcag cacaaagaaa
     1981 tggtgctaag gttccccgcg ttcgtaatgg attcacatct atggatattg gtctaaatta
     2041 ccagattctg ccagacacgc tgattaattt tgccgttctt aacgtcacag acagaaagag
     2101 cgaggatatc gataccattg atggtaactg gcaggtcgat gaaggacgcc gttattgggc
     2161 taatgtaaga gtatccttct gagagtatat aaagacaacc tgaggccgtg agcctcaggt
     2221 accagtgtag taatcagtaa tgtcagtttc caggttgtaa tgcttttaat cttgattaaa
     2281 ataat
//
LOCUS       DBBGLQ010000284         2199 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.286, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000284 DBBGLQ010000000
VERSION     DBBGLQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.286"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..553
                     /gene="asrB"
                     /locus_tag="ACIYSB_005526"
     CDS             <1..553
                     /gene="asrB"
                     /locus_tag="ACIYSB_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HGC5432319.1"
                     /translation="GDRVWLRGCYGNGYPVDAFRHKSLVVIAGGTGVAPVKGLMRYFV
                     ENPQEINRLDMVFGYRNRDCVLYKQEMANWHRHHNLILTLDEGEADERYRTGRVTDHL
                     AALTFEDISTMQAIVVGPPVMIKFTVQMLLEKGLQPEQIWVDYERRMACAVGKCGHCH
                     MGDVYVCVDGPVFNYAVARQFAD"
     gene            564..1574
                     /gene="asrC"
                     /locus_tag="ACIYSB_005527"
     CDS             564..1574
                     /gene="asrC"
                     /locus_tag="ACIYSB_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HGC5432320.1"
                     /translation="MSVDIDIIKARANNEYRLSKVRGEAMISVRIPGGILPAHLLTVA
                     REIAETWGNSQIHLTTRQKLAMPGIRYEDIDRVNAALEPFIREIEMELCDVQVDDPKA
                     GYLAIGGRNIVACQGNRICQKANTDTTGLSRRLEKLIYPSAYHLKTVIAGCPNDCAKA
                     SMSDFGIIGVAKIRFTAERCIGCGACVKACAHHAVGCLSLKNGKAIKEESLCIGCVEC
                     VLACPTLAWQRQPQEFWQVRLGGRTGKKTLRAGKVFLNWVTEDVIRQVISNLFEFEKE
                     MLDGKPVYLHMGHLIDKGGYLRFKERVLRGVQLNPEATVAERIYWAEDESVARLHLKS
                     VR"
     gene            complement(1724..>2199)
                     /locus_tag="ACIYSB_005528"
     CDS             complement(1724..>2199)
                     /locus_tag="ACIYSB_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5432321.1"
                     /translation="LSDLSGRAKSHILTAGIRGGWKMHAAPLDMSITPTVSLNGARVD
                     GNRLQGRERSVKLHDGDALWLKAGVEAEKVSGNMTLKAGVWRNITLNDMPGMTLRDDW
                     KARHYDAEKADRYTVSFGVNGKLTEKLSVQVKVNSSFDGYFKTDAEGILGIRYDF"
BASE COUNT          515 a          581 c          597 g          506 t
ORIGIN      
        1 gggcgacagg gtatggctgc gtggctgcta tggtaacggc tatcctgtcg atgccttccg
       61 ccacaaatct ctggtggtca ttgccggcgg taccggcgtt gcaccggtca aggggttaat
      121 gcgctatttc gtcgaaaacc cacaggaaat caaccggctt gacatggttt tcggttacag
      181 aaaccgggac tgtgtgctgt ataaacagga gatggcgaac tggcaccggc accacaatct
      241 cattctgacc ctggatgaag gcgaggcgga tgaacgttac cgtaccgggc gggtcactga
      301 ccatctggca gcactgacat ttgaagatat cagcaccatg caggcgatcg tggttggtcc
      361 tccggtgatg attaaattca ccgtacagat gttgctggaa aaagggctgc agccggagca
      421 aatctgggtg gattatgaac gccggatggc ctgtgcagtg gggaaatgcg gtcactgcca
      481 catgggggat gtctatgtct gcgtcgatgg tccggttttc aactatgccg tggcacgtca
      541 gtttgcagac tgaggaaaaa atgatgagtg tggatattga tatcattaag gcccgggcaa
      601 acaacgaata tcgcctttcg aaagtccgtg gcgaggccat gataagcgta cgtatacccg
      661 gcggcattct gcccgcgcac ctgctgacgg tggcacgtga gattgcggaa acctggggta
      721 acagtcagat ccatctgacc acccgtcaga aactggccat gcccggtatt cgttacgaag
      781 acatcgaccg ggtgaatgcc gcactggagc ctttcattcg cgaaatcgaa atggaactgt
      841 gcgatgttca ggttgatgat ccaaaagccg gatatctggc gattggcgga cgtaacattg
      901 tcgcctgcca gggtaaccgg atttgccaga aagccaacac cgacaccaca ggtttatccc
      961 ggcggctgga aaagctgatt taccccagtg cctatcacct gaaaaccgtc atcgccgggt
     1021 gtccgaatga ctgtgccaaa gcgtccatgt cagattttgg cattattggt gtggcgaaaa
     1081 ttcgctttac tgccgaacgt tgcattggat gtggcgcctg tgtgaaagcc tgtgcgcacc
     1141 acgccgtggg gtgcctgtca ttaaagaacg gcaaagccat aaaggaagag tcgctctgta
     1201 tcggatgcgt agaatgtgtt ctggcatgcc ccaccctcgc ctggcagcgt cagccacagg
     1261 agttctggca ggttcgcctg ggaggaagaa ccggtaaaaa aacgctacgg gcagggaaag
     1321 ttttccttaa ctgggttaca gaggatgtca tcaggcaggt catatcaaac ctgttcgaat
     1381 ttgaaaaaga aatgctggat ggtaaaccgg tttacctcca tatggggcac cttattgata
     1441 aggggggcta tttgcgcttt aaagagcggg tactacgcgg cgtacaactg aatcccgaag
     1501 ccacagtggc ggagcggatt tactgggcag aagatgaatc tgtcgcccgc ctccatctta
     1561 aatcagtcag gtgacatgaa atcattatca gtcaccagcg ttcagtcagg agtgaaaccg
     1621 gatattccgc acaggcagcg ttgcctgccc tgaccatact gccctgtcag gaggggcgct
     1681 gttctgcccc tccctctccc tgcagcatca gcgatggctg ttatcagaag tcataccgga
     1741 tacccagtat tccttccgca tccgtcttaa agtaaccatc aaaactgctg ttcaccttta
     1801 cctgcactga cagtttctct gtcagctttc cgttaacacc aaacgaaaca gtatagcgat
     1861 cagccttctc tgcatcgtag tgacgggctt tccagtcatc acgcagggtc attccgggca
     1921 tatcgttcag cgtgatattc cgccagacac ctgccttcag cgtcatattt ccggaaactt
     1981 tttctgcctc aaccccggct ttcagccaca gggcatcgcc atcatgaagc ttcacgctcc
     2041 gctcccggcc ctgcagacga tttccgtcca cccgggcacc gttcagtgaa acagtcggtg
     2101 ttatcgacat atcaaggggg gctgcatgca tcttccaccc accacggata cccgctgtca
     2161 ggatatggct ttttgccctg ccggacaggt cactcagct
//
LOCUS       DBBGLQ010000285         2198 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.287, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000285 DBBGLQ010000000
VERSION     DBBGLQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.287"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            61..1230
                     /locus_tag="ACIYSB_005529"
     CDS             61..1230
                     /locus_tag="ACIYSB_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGC5432322.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSVHKKGHLQEFYRVNVIKRHP
                     MAERYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEI
                     LSLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFNYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLR
                     AYHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISH
                     ASVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            1272..1562
                     /locus_tag="ACIYSB_005530"
     CDS             1272..1562
                     /locus_tag="ACIYSB_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432323.1"
                     /translation="MKHLSSHAATADNNRIVTIEDVYTIAFSQPIHTESGLGQRWMLI
                     HTGEYESASDAKRFLDTLAIGHCACMVTPSPVLDHDTWLHIRLLMGEIRDAR"
     gene            complement(1654..1974)
                     /locus_tag="ACIYSB_005531"
     CDS             complement(1654..1974)
                     /locus_tag="ACIYSB_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432324.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLVYIVCLYIGTLVKNIFIRIFAFSIWALSAEGAIIYNMI
                     KILS"
BASE COUNT          652 a          582 c          420 g          544 t
ORIGIN      
        1 cgatattgca tttccgtttc actattccta ccttccccgt ttttatcgtg gaaggcagat
       61 atgtttcgaa aaatcaaaat tcgcaaaatg actctcaacc gggcgctgga caaatacctg
      121 aaaaccgttt ccgtccataa aaaaggtcat ctgcaggagt tttatcgcgt caatgtgatt
      181 aaacgtcacc ctatggccga gcgctatatg gatgaaatca ctaccattga tatcgccggt
      241 taccgggatc agcgtttagc tcaaattaac ccacgaacag ggcgtcaaat caccggaaat
      301 accgtccgtc ttgaacttgc ccttttatca tcattattca atatcgcccg tgtggaatgg
      361 ggaacatgcc gtatgaatcc tgttgaactg gtccgaaaac cgaaaatcag taatggccgc
      421 gaccgccgac tgacttctgg tgaagaacgc cgcctttccc gctatttcag ggacaaaaat
      481 ccgcagctct acgttatctt ccatctggct ctggaaacag caatgcgcca gggagaaatt
      541 ctctcattac gctgggaaca cctcgatttg cagcacggcg tggcgcatct tcctgaaacg
      601 aaaaatggct caccacgcga cgtaccactc tcccggaaag cacgtaacta cctgcaaatt
      661 ctgccacaac agataaacgg caatgtgttc aattacacct cgtccggttt caaaagcgcc
      721 tggcgtaccg ctcttctgga tctgaaaata gaaaatctgc attttcacga tctccgccat
      781 gaggccatca gccgtctgtt cgagctcggc acactgaacg ttatggagat tgcagccatt
      841 tccggtcacc gctccctgaa catgttgaaa cggtacacac atctccgggc atatcacctg
      901 gtcagtaaac tcgatgcaag gcgtaaacag accagcaaaa tttcccctta ttttgttccc
      961 taccctgcaa ctgtcagaaa cagaaacgga gtgttcattg tgacactgca cgatttcgac
     1021 cttgaaagcc gtgctgaaac ccgggaactg gcaatatctc acgccagcgt attgttgctg
     1081 cgcgcactgg cccaggctgc tcagcggggt gaacgcgtcc ccacaccagg cgagctaccc
     1141 gcaaacattg atgaacgggt catgatttgt ccgttaacaa acagccagtt catgacaaaa
     1201 gcgaacagta ttttcatatc ctcctcctga acaaataatt tttgttcaaa attaacgcta
     1261 tgagaccctt tatgaaacac ttatcctccc atgcagccac tgcggataat aatcgtatcg
     1321 ttacaattga agacgtttac acaatcgcat tctcgcaacc aattcacacc gaatcagggt
     1381 tggggcaaag gtggatgttg atccatactg gtgaatatga aagtgccagc gatgcaaaac
     1441 gcttccttga tacgctcgcc atcgggcact gtgcttgcat ggtaacacca tcacctgttc
     1501 tggatcatga cacctggctg catatccgcc tgctgatggg cgagataagg gatgccagat
     1561 aaacattcat ggccccaacg cataaaaata acgacgggga ctctggcagt ggtaccactc
     1621 tttttacata gagccaatat ataaacaact tttctatgat aatatcttta tcatgttata
     1681 aataattgcc ccctcagcag aaagggccca aatagaaaaa gcaaaaatcc ttataaaaat
     1741 attctttaca agcgttccta tatacaaaca aactatataa accagccaaa acagaatgac
     1801 tgcaaaagaa acacacacgg cccccataaa aataaatgtc atcgaatccc atggtttcac
     1861 cagtacaacc ggaaccatgg ctgccagaac aataagagca gcattaccca acagcgtctc
     1921 tcgaataata tcccttataa tctccagttt ttctttatgc tgtttaattt tcatacgaac
     1981 ctcgttgatt tcatcccgaa aaggaaaaga ttatataacg aaatactttc aatctcctga
     2041 ctccgtctgc tctgcagcat aatgccagac cagcggcata ctaaatcacg tcctgtccga
     2101 atccttttca ttcgccctta aaacagagtt gcaaacactc ttgaaacaca ctcgatcata
     2161 acgatcgaaa taactcgctt ccgtttaaaa aacactct
//
LOCUS       DBBGLQ010000286         2197 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.288, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000286 DBBGLQ010000000
VERSION     DBBGLQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.288"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            2..2197
                     /locus_tag="ACIYSB_005532"
                     /pseudo
     CDS             2..2197
                     /locus_tag="ACIYSB_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491406.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          782 a          421 c          395 g          599 t
ORIGIN      
        1 tatgttttct ataaaaccag gacccagaaa tttacctatc gacaacccca cattgttatc
       61 atggaacatt actgacgggg atctaaattc caaattaaat acattagaat atctaaactg
      121 tataacaaat attattaatt cttgtggagt ttaccctcaa ggattaaaag acagagaaat
      181 tatatcaact tttcacgcag aaaaagttat taatgatctg ttaaaaaacg attataaaat
      241 ttccctttct ccagatacaa cttaccgaga gttgaataaa gcagcacagc gtagcattac
      301 agcgccagac aggataggag aaagaaaaac ataggtttat caacgagata caatgattga
      361 aagaggtgat aacagcggtg tttatcagta tggtcgtgct gaacatttca cccacattat
      421 atctgacaaa ccttccccaa aagataaata tgttgcatat gctattaaca ttcctgacta
      481 tgagctggca gccgatgtat ataatattaa cgtgacgtca ccttccggac agcaagaaac
      541 atttaaaata ttaatcaatc tagaacatct acggcaaaca cttgagcgta aatctcttac
      601 tgctgttcag aaatcacaat gtgaaatcat cacccccaaa aaacctggcg aagcgattct
      661 tcatgctttt aatgccacct accagcagat cagagaaaat atgtctgaat ttgcacgttg
      721 ccattatggg tatatacaaa tccctccagt gacaactttc cgcgccgacg gaccagaaac
      781 tcccgaagaa gaaaagggtt actggtttca tgcttatcaa cccgaagatc tttgtaccat
      841 ccataatcca atgggagatt tgcaggattt tattgcattg gttaaagatg ctaaaaaatt
      901 tggtatcgat atcattcctg attatacctt taactttatg ggaattgggg gtagtggtaa
      961 aaatgacctg gattatccct ctgctgatat acgagcgaag atcagtaaag atatagaagg
     1021 tggtatccct ggctattagc aaggtcaggt tttgattcca ttcattaaag atccagtaac
     1081 aaaagaacgt aaacaaatcc atccagaaga tatacatctc actgcaaaag acttcgaagc
     1141 aagtaaagat aacatctcta aggatgaatg ggaaaacctc catgcattaa aagaaaagcg
     1201 tttaaatgga atgcctaaaa caacacccaa aagtgaccag gttattatgt tgcaaaatca
     1261 atacgttcgt gaaatgcgaa aatatggcgt acgaggttta cgttatgatg cggcaaaaca
     1321 ctcaaaacat gaacaaatag aaagatcaat aaccccaccg cttaaaaatt ataatgagcg
     1381 gttacacaat actaacttat ttaacccaaa atatcataaa aaagccgtta tgaattacat
     1441 ggaatatctg gtaacttgtc agttggatga acaacaaatg tcatcactgc tttatgaaag
     1501 agatgattta agcgccattg atttttcatt gctcatgaaa acgataaaag ccttttcatt
     1561 tggtggagat cttcaaaccc ttgcatcaaa accgggttcc acaatctcaa gtatcccatc
     1621 agaaagacgg atattgatta acattaacca cgattttcct aacaatggta atcttttcaa
     1681 tgactttcta tttaaccatc aacaagatga acaattagca atggcatata tagccgctct
     1741 gccgttcagc aggcctttag tttactggga tggccaagta ttaaaatcaa cgactgaaat
     1801 taaaaattat gatgggtcca cgcgtgtcgg cggtgaggcg tagcttaata aaggttgctc
     1861 tacctatcag cagctctaca atgaattcca cgcattatat atagataaag caggaatatg
     1921 gagcgcattt gagggtgtat ctgcaactaa aaacgttctg gcctttagtc gtggggattc
     1981 tgtgaacatt aatcactctc ctcatgatgg actagttata ataaataaag gaaacgaaga
     2041 agttgaaggt acctggccta acaaattgca acctggaata tacaaaaaca tggggagtaa
     2101 tagcgttaac attattatta ataatacccg aaaaattatc ccccctggta aagtatttac
     2161 gcttagaggc ggaactctaa atatcaatat tcctggg
//
LOCUS       DBBGLQ010000287         2194 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.289, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000287 DBBGLQ010000000
VERSION     DBBGLQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.289"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>2194
                     /gene="rhsC"
                     /locus_tag="ACIYSB_005533"
     CDS             <1..>2194
                     /gene="rhsC"
                     /locus_tag="ACIYSB_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HGC5432325.1"
                     /translation="WLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTY
                     DDKYRGRMVAHRHTGRPEICYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRR
                     EVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLIT
                     RITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRYRYDN
                     PHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLS
                     QYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQ
                     GGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLI
                     RSEDEGLVTHWHYDEADRLTHRTVNGETAERWQYDERGWLTDISHISEGHRVTVHYGY
                     DSKGRLASEHLTVHHPQTNELLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWL
                     SGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWN
                     DNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYTTDPAGNRLPDPELHPD
                     SALSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLV
                     HYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVT"
BASE COUNT          527 a          672 c          655 g          340 t
ORIGIN      
        1 tggctgacgc acgacccgga atacccggag aatttacctg ccgcgccgct ggtgcgctat
       61 ggctggacgc cccgcggcga actggcggcg gtgtatgacc gcagcaatac acaggtgcgc
      121 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccga
      181 ccggaaatct gttaccgtta cgacagcgac gggcgggtga cagaacagct aaacccggca
      241 ggcttaagtt acacgtatca gtatgagaag gaccgcatca ccatcaccga cagcctgaac
      301 cgccgtgaag tcctgcacac gcagggtgaa ggcgggctga agcgggtggt gaaaaaggaa
      361 cacgcggacg gcagcgtcac gcagagtcag tttgacgcgg tgggcaggct cagggcacag
      421 acggatgccg caggcaggac aacagaatac agtccggatg tggtgacggg cctcatcacg
      481 cgcatcacca cgccggatgg cagggcatcg gcgttttact ataaccacca cagccagtta
      541 acgtcagcca ccgggcctga cgggctggaa atacgccggg aatatgatga atggggccgt
      601 ctgattcagg aaactgcccc tgacggcgat atcacccgct accgttatga taatccacac
      661 agtgacttac cctgcgcaac ggaagatgcc accggcagcc ggaaaaccat gacgtggagc
      721 cgttacggtc agttgctgag cttcactgac tgttccggtt atgtaacccg ctatgaccat
      781 gaccgttttg gtcaggtgac ggcggtgcac cgcgaggaag ggctgagtca gtaccgcgca
      841 tacgacagcc gtggacagtt aattgccgtg aaagacacgc agggccatga aacgcggtat
      901 gaatacaacg ccgccggtga cctgaccacc gtcattgccc cggacggcag cagaaacggg
      961 acacagtacg atgcgtgggg aaaagccatc tgtaccacgc agggcggtct gacgcgcagt
     1021 atggaatacg atgctgccgg acgggtcatc cgcctgacca gtgaaaacgg cagccacacc
     1081 accttccgtt acgatgtact cgaccggctg atacaggaaa ccggctttga cggccgcaca
     1141 cagcgttatc accacgacct gaccggcaaa cttatccgca gcgaggatga ggggctggtc
     1201 acccactggc actatgacga agcagaccgc ctcacgcacc gcaccgtgaa tggcgaaacc
     1261 gcagagcggt ggcagtatga cgaacgcggc tggctgacag acatcagcca tatcagcgaa
     1321 gggcaccggg tgacggtgca ctatggttat gacagtaaag gccgcctcgc cagtgaacac
     1381 ctgacggtgc atcatccgca gacgaatgaa ctgctctggc agcatgagac cagacatgcg
     1441 tacaacgcac agggactggc gaaccgctgt ataccggaca gcctgcccgc cgtggaatgg
     1501 ctgacctacg gcagcggctg gctgtcaggc atgaaactcg gcgacacacc gctggtggag
     1561 tacacccgcg accgcctgca ccgggaaacg ctgcgcagct tcggccgtta tgaactcacc
     1621 accgcttata cccctgccgg gcagttacag agccagcacc tgaacagcct gctgtctgac
     1681 cgcgattaca cctggaacga caacggcgaa ctcatccgca tcagcagccc gcgccagacc
     1741 cggagttaca gctacagcac caccggcagg ctgaccggcg ttcacaccac cgcagcgaat
     1801 ctggatatcc gcatcccgta taccacagac ccggcaggta accgcctgcc cgacccggag
     1861 ctgcacccgg acagcgccct cagcatgtgg ccggataacc gtatcgcccg tgacgcgcac
     1921 tatctttacc ggtatgaccg tcacggcagg ctgacagaga aaaccgacct catcccggaa
     1981 ggggttatcc gcacggatga tgagcgcacc caccggtacc attacgacag tcagcaccgg
     2041 ctggtgcact acacgcggac acaatatgca gagccgctgg tcgaaagtcg ctatctttac
     2101 gacccgctgg gccgcagggt ggcaaaacgg gtatggcggc gtgaacggga cctgacgggc
     2161 tggatgtcgc tgtcacggaa accgcaagtg acct
//
LOCUS       DBBGLQ010000288         2170 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.290, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000288 DBBGLQ010000000
VERSION     DBBGLQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.290"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..357)
                     /locus_tag="ACIYSB_005534"
     CDS             complement(<1..357)
                     /locus_tag="ACIYSB_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGC5432326.1"
                     /translation="MSLKAIAAELGISVTTVSRALGGFSDVSASTRARVEAEARRRGY
                     RPNTLARRLKTGKTDAVGLVFPEHAVLFSSSVFVDMVSCISRELARHDIDLLLIADDE
                     RADSHSYMRLVESRRVD"
     gene            783..1990
                     /locus_tag="ACIYSB_005535"
     CDS             join(783..1104,1104..1990)
                     /locus_tag="ACIYSB_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGC5432327.1"
                     /translation="MAGVLLGKEVRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYQEGSLTAVAAGEDVVPASELAAAIKQINQLQRLLGKKTMEVELLKEAV
                     EYAQSPKMDSACALVTFGQGIAPVSRVLGVSRAQLTLRMKASDNKTDKRRQRRDEAAD
                     AEVLSRILDIIGDMPTYGYRRVWAIPRRQSRNEGLPFVNAKRVYRIMSENSLLLLHDK
                     PSRPQRAHKGRVEVNKSDQRWCSNGFEFGCDDGEKVRVTFALDCCDREAIDWAASTGG
                     YDKSTVQDVMLGAIEKRFGDKVPEQSIQWLTDNDSAYRAHETRQFARELNLEPCTTAV
                     SSPQSNGMAERFVKTMKEDYIAFMPKPNVRTAVHNLAVAIEHYNENHPHSALGYRSPR
                     EYRRQRVTLT"
BASE COUNT          593 a          502 c          580 g          495 t
ORIGIN      
        1 atcaacccgg cggctttcga ccagacgcat atagctgtgg ctgtcagccc gttcatcatc
       61 ggctatcagc agtaagtcaa tatcatggcg ggccagttcc cggctgatac aactgaccat
      121 atccacaaac acactactgc tgaacagtac agcatgttca gggaagacca ggccgaccgc
      181 atcggtttta ccggttttga gacgtcttgc cagggtattc gggcgataac cacgacgccg
      241 agcttccgct tctacacgtg cacgggtcga agcggaaacg tccgaaaatc cgccaagcgc
      301 ccggctgact gtggtgacag agatcccgag ttcggcggcg atagccttga gtgacatatt
      361 ttctcctgcg gataactgaa cagccctgtc cctgatgcgg gtgatccggg caacaaacct
      421 gacagagacg cctgcataag tggtcgcgct gtaccagtat gagcctccat catcctgatt
      481 tgtgtattaa ctttcaatac cggaggacga tttgattacc ggttgttagc aggagtgcga
      541 atattagacc gacggtggag atctaagact cagtgagcat agcgatacac tatattactg
      601 taattaagcc agtaaacttc ctgttcactg gcttaaacgc taatgatgta tgcagttgaa
      661 caagctttta aactaatgat taattgatat atgagctggc cccctgaaac accagacagt
      721 agatgtatct taaaataaga ggtaggcttg gatatatgtc taacactagt tctaattttg
      781 agatggctgg cgttttgtta ggaaaagagg tgcgtaaacg taaaacacct caggaaaaaa
      841 tcgcaattat tcaacagact atggagccag gcatgacggt gtctcacgtg gcgcgtttgc
      901 atggaattca gcccagcctg ttgtttaagt ggaaaaaaca ataccaggaa ggaagtctca
      961 ctgctgtggc tgcgggtgaa gacgttgttc ctgcttctga actggccgct gcgataaagc
     1021 agattaatca gcttcagcgt ttactgggca agaaaacaat ggaagttgag ctcctcaaag
     1081 aagcggtgga gtacgcgcaa tcgcgaaaat ggatagcgca tgtgcccttg ttaccttcgg
     1141 acaaggaata gccccggtaa gccgtgttct gggcgtgtcg cgtgcgcagt taacattacg
     1201 gatgaaggca tctgataaca agacagataa acggcgtcag agacgcgatg aggctgcgga
     1261 tgcagaagtc ttatcgcgca tcctggatat cattggcgat atgccaacat atgggtatcg
     1321 ccgtgtctgg gcgataccca gacggcagtc ccgaaatgaa ggcctgccgt tcgtgaatgc
     1381 aaaacgcgtt taccggataa tgagcgaaaa cagcctgttg ttgttgcatg acaaaccatc
     1441 acgacctcag cgggcacata aaggccgtgt agaggttaat aaaagcgacc agcgttggtg
     1501 ttccaatggc tttgagttcg gttgcgatga tggcgaaaaa gtccgggtta cgttcgccct
     1561 ggactgttgc gatcgtgaag ctatcgactg ggcagccagt acgggaggtt acgataaatc
     1621 gacagtgcag gatgtgatgt taggcgcgat agaaaaacgc tttggcgata aagtgccgga
     1681 acagtcgatc cagtggctga cagataacga ttcagcgtac agagcgcatg aaacacggca
     1741 gttcgccaga gagctgaatc tggagccctg tacgacggca gtcagcagtc ctcagagtaa
     1801 tggaatggca gaacggttcg tgaaaacgat gaaggaagac tacatcgcgt tcatgccgaa
     1861 accgaatgta agaacggcag tgcataacct tgcagtggcg atcgagcatt acaatgaaaa
     1921 ccatccgcac agtgcgttgg gttatcgctc tccgcgagaa tatcgacgtc agcgggtaac
     1981 gttaacttaa gatacaatcc ctgtctggag ataaggggtc aagaacaaaa tcgcgccctg
     2041 gctgtccaga ttgcattccc ggatgcgtcg ttccgcaaac cgggtcagtt gttcatagct
     2101 gagtgaaaac accctttctt catccatcct gcgcgctcca tctgataatg ttctgacagg
     2161 taaaaatcaa
//
LOCUS       DBBGLQ010000289         2140 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.292, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000289 DBBGLQ010000000
VERSION     DBBGLQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.292"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..444
                     /locus_tag="ACIYSB_005536"
     CDS             <1..444
                     /locus_tag="ACIYSB_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HGC5432328.1"
                     /translation="SFIQNTGIARILPLRNGRLITCLTDNQEPSPCLIWLPAPDITAT
                     FPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYYSGSWYLIALQKGKLQVFPLAD
                     IKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIHKLINTFNL"
     gene            592..882
                     /locus_tag="ACIYSB_005537"
                     /pseudo
     CDS             592..882
                     /locus_tag="ACIYSB_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            956..2133
                     /locus_tag="ACIYSB_005538"
     CDS             join(956..1214,1214..2133)
                     /locus_tag="ACIYSB_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGC5432329.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGIAPSATPITPEQQRIRELEKQVRRLEEQNTIFKKGYRALDVRLTERF
                     TIVSRLSDSHSVVSLCSALGIHRSSYRYWRKRRDTVNSARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNACQEHTCLPNLLERQF
                     AVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAY
                     EVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTDGYVGKDEARQQISGYILNYYNSVRPHHYNGGLTPEESENRYRFYCKTVANIT
                     "
BASE COUNT          553 a          548 c          551 g          488 t
ORIGIN      
        1 tcttttatac agaataccgg gatcgcacgg atacttccgc tccggaacgg acgactgata
       61 acctgtctta ccgacaacca ggagccctct ccctgcctta tctggctacc ggcgccggat
      121 atcactgcaa cgttccccga gtgtttctcg caactcatcc tggcaataag acagtgtatc
      181 cacatctctc tgatgactga gcgatggtat ccgtcactgg agccctgccg gctcatttat
      241 tacagcggta gctggtatct gatcgcgtta cagaagggaa aactgcaggt ctttcctctg
      301 gcagatatca aatcagtcag cctgacatca gaacggtttg aacggagagg ccacatccac
      361 agtctggtcg ctgaagagcg ttttatctcc gccctgccac atttctcttt catccataaa
      421 cttatcaaca cctttaacct gtgatcgccg gcctgccaaa gccgtcccga caggtatgga
      481 gacaatatgt tgaacagaaa actaaatata cggctacgtc attccctgaa cagtcactgc
      541 ataccttcca tcattatcaa taacaccgta cgttcatttc agaggtcagt catgaatacc
      601 agagctcttt ttcccctgct gttcactgtg gcatcattct ccgcctccgc cggcaactgg
      661 gctgtcaaaa acggctggtg tcagaccatg acggaagatg gtcaggcgct ggtaatgctg
      721 aaaaatggca cgattggtat taccggcctg atgcagggat gcccgaatgg tgtacagacg
      781 ctcctgggca gccgtatcag tattaacggt aacctgatcc ccacatcaca aatgtgtaat
      841 cagcagacgg gattcagggc tgttgaggtg gaaatcggac aggtgtagtg gcacagtaaa
      901 tttggccacc tgattaaagg tgatattctc acctcaacac aaaacaggtg acttaatgaa
      961 caagaaaacc aaacgtactt tcacccctga gttcaggctg gaatgtgcac agctgattgt
     1021 tgataagggc tattcgtatc gacaagccag tgaggcgatg aatgtcggtt ctaccacgct
     1081 tgagagctgg gtacgtcagc tcaggcgaga gcgccaaggt attgcgccct ctgcaacacc
     1141 cattacccct gaacagcaac gtattcgcga gctggaaaag caggttcgcc gactggagga
     1201 acaaaatacg atattaaaaa aggctaccgc gctcttgatg tccgactcac tgaacggttt
     1261 acgatagttt ccagactgag tgacagccac tcagttgtca gcctctgttc cgcactggga
     1321 atacaccgta gcagttaccg gtactggcga aaacgacgcg atacggttaa ttcggctcgc
     1381 gtcaggttgt gcagcgaaat acgccgggca tggaaccaga gccggggctc tgcaggcgcg
     1441 cgcacgctgg ctgaaatgct gacccaaaac ggcgtcccga tgagccgtta tcgtgccgga
     1501 cggctgatga aatacctgaa cctgagcagt tgtcagcccg gaaaacatca atacaaaaat
     1561 gcctgtcagg agcatacgtg tttgccgaat ctgctcgagc gtcagttcgc tgtaccggag
     1621 ccagacaggg tatggtgcgg agatattacg tatatctggg caggaaatcg ctggtgctat
     1681 ctggcggtcg tcatggatct ttttgcccgc agggttatcg gctggagcct gtcagcgaat
     1741 gccgatactg cactgataag cagtgccttg cggatggcgt atgaggtgcg tggtcagccg
     1801 cgggacgtca tgttccatag cgaccaggga agtcagtata caggactgaa atatcaacag
     1861 cttctctggc gttacaggat aaagcaaagt gtcagccgac ggggaaactg ctgggataac
     1921 agtcctatgg aacgcttctt ccgcagtctg aaaacagaat gggtgccgac ggatggttac
     1981 gtaggcaagg acgaggcccg gcaacaaatt agcggttata tcctgaatta ttacaatagc
     2041 gtcaggcctc accattacaa cggtgggttg acgccggaag aatcagagaa cagataccgt
     2101 ttttactgta aaaccgtggc caatattact tgaccactac
//
LOCUS       DBBGLQ010000290         2101 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.294, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000290 DBBGLQ010000000
VERSION     DBBGLQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.294"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..365
                     /locus_tag="ACIYSB_005539"
     CDS             <1..365
                     /locus_tag="ACIYSB_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432330.1"
                     /translation="LTAAAMWQSYRLGRAGEARDAAEQQLGEYSRALRESSAVISDAS
                     AVLHEVMTQRQKRAQEGEVRREQLREIAATDECAGTLPDHRFTDRLREHTERATARAL
                     LPVHSGGTDDGNTAPALP"
     gene            497..961
                     /locus_tag="ACIYSB_005540"
     CDS             497..961
                     /locus_tag="ACIYSB_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432331.1"
                     /translation="MLKSKSLSTHMSTACRWCRANPEKFTVFIERGGIETTGETPTFV
                     YNYRLVMFVMDYTGDLDNLTLPLIVWLAENQPQLLLNPERNNDIQFTAAINDDDSADV
                     LFELPLRERVRVTRRDDGTLMTEHLPEPKSRIPQDGDWSHILEDVTWNIVTG"
     gene            1019..1633
                     /locus_tag="ACIYSB_005541"
     CDS             1019..1633
                     /locus_tag="ACIYSB_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGC5432332.1"
                     /translation="MSNMFVNIRLATSPQAVKRIAAHVGKELRRVNQSRMRAQKAPDG
                     SVWTRRKRRISRLQERISFIWQNQNRTLKNWHHGSGKYGQTITGWDEDRSGIRTFYRS
                     DILRFLEIRTRRINRDTTKTVPMFAKLRTARYLKVKADASGVTVGYSGVAARIARVHQ
                     FGERDRVAAGMYTDYPARELLGITPADERLIQRIIMDNIARAVA"
     gene            1630..>2101
                     /locus_tag="ACIYSB_005542"
     CDS             1630..>2101
                     /locus_tag="ACIYSB_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGC5432333.1"
                     /translation="MNAELMRLISNIIRTGVIFDVNPQTWEVRVRSGGLETGWLRWST
                     ARAGAFSVWVPPATGEQVAFVCIGGNPETAIIIGSLWSDDIPAPGSTLKEIIMTAPDG
                     AVFRYDADAGALEVKGIKTASIQADTRITLDTSEVVCTRKLITATLEVQNGGEMR"
BASE COUNT          489 a          598 c          654 g          360 t
ORIGIN      
        1 ccctgacggc ggcggccatg tggcagtcat accggctggg gcgtgccggt gaggcacgtg
       61 acgccgcaga gcagcagctg ggcgaataca gccgggcgct gcgtgagtcc tccgccgtta
      121 tcagcgatgc ctccgccgta ctgcatgagg tgatgacgca gcgacagaaa agggcacagg
      181 aaggagaagt cagacgtgaa cagcttcgcg aaattgctgc gacggatgag tgcgccggca
      241 ctcttcctga ccaccgtttt actgaccggc tgcgcgaaca cacggaacgc gccactgccc
      301 gcgccctcct tccggttcat tccggcggga ctgacgacgg gaacacggcc cccgcccttc
      361 cctgaacacc ccacctgggg aaatatcggg atatggagcg acagcctcct ggatgcactg
      421 gaaacctgca acgcagacaa acgggccatt gaggcgatgg agcgccggag actcaaccga
      481 cagaacggag aggcgcatgc tgaaaagtaa atccctgagc acacacatga gtacagcgtg
      541 ccggtggtgc agggccaacc cggaaaagtt caccgtgttc attgaacgtg gcgggataga
      601 gaccaccggc gagacgccga cgttcgtgta caactaccgg ctggtgatgt tcgtgatgga
      661 ctacacgggc gatctggaca acctgacgct tccactgatt gtctggctgg cagagaacca
      721 gccgcagttg ctgcttaatc ctgagcgcaa caacgatatt cagttcacgg cggccattaa
      781 cgacgacgac agcgcggacg tgctgtttga gctgccgtta cgtgaacgcg tgcgcgtcac
      841 ccgcagggat gacggcaccc tcatgacaga acatctaccg gagccgaaat cacgcatccc
      901 gcaggacggc gactggtcac acattctgga ggatgtgacg tggaacattg tcaccggata
      961 acacgcggag aatgttatgg ggaaactgag ataccgtaac ctcgttcccg ctgaaggaat
     1021 gagtaacatg tttgtcaata tccggctggc aaccagcccg caggcagtga agcgcattgc
     1081 cgcgcatgtg ggtaaggagc tgcggcgcgt gaaccagagc cggatgcgcg cccagaaagc
     1141 gccggacggt tcggtgtgga cacgccgtaa acgccgtata tcacgccttc aggagcgcat
     1201 cagctttatc tggcaaaacc agaaccgcac cctgaaaaac tggcatcacg gttcaggaaa
     1261 atacggccag accatcaccg gctgggatga agaccggagc ggcatacgta ccttttaccg
     1321 gagtgatatc ctccgtttcc ttgaaatcag gacacgccgc atcaaccgtg acaccacaaa
     1381 aacggtgccg atgtttgcga agctgcgcac ggcccgttat ctgaaagtga aggcggacgc
     1441 atcaggtgtg acggtgggct acagcggcgt ggcggcacgc attgcccgcg ttcaccagtt
     1501 cggggagcgc gaccgggtgg ccgcagggat gtatacggat tatccggccc gtgaacttct
     1561 tggaataaca ccggcagatg aacgacttat tcagcgtatc attatggaca atatcgcgag
     1621 ggctgtggca tgaacgcgga attaatgcgc cttatcagca acatcatcag aaccggcgtg
     1681 attttcgacg tgaacccgca gacctgggag gtccgggtgc gcagtggcgg actggagaca
     1741 ggctggcttc gctggagcac cgcgcgggcg ggcgcgttca gtgtctgggt gccgccagcc
     1801 accggtgaac aggtggcgtt cgtctgtatc ggtggtaatc ccgaaaccgc gataatcatc
     1861 ggcagcctct ggagcgatga tatcccggca ccgggcagca cactgaagga aatcatcatg
     1921 acagcaccgg acggtgcggt attccgctac gatgcagacg ccggtgcact ggaggttaag
     1981 gggattaaaa cagccagcat tcaggccgac acgcgcatca ccctggacac gtcggaggtg
     2041 gtgtgtaccc gcaaacttat caccgccacc ctggaggtgc agaacggcgg cgagatgcgc
     2101 g
//
LOCUS       DBBGLQ010000291         2086 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.295, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000291 DBBGLQ010000000
VERSION     DBBGLQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.295"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            76..906
                     /locus_tag="ACIYSB_005543"
     CDS             76..906
                     /locus_tag="ACIYSB_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGC5432334.1"
                     /translation="MAFNFHRAAEGIVLANSHRGYSSCAPENTFPAFEAARLAGTHCI
                     EIDIHLTADNQLVVTHDHRIDRVSTGHGFVEQMTYPQLLACDFGVKYHPSFAGTRIPL
                     LADVLRWAVENSMGLIVEVKQRNRHDEFVAALVALLHEHLDALGHIQLLGFNHVLINR
                     VKAQIPQLALQVVTLERYNNQLAAVQQSNAGCVCFEFEFAHIDDLRAYKNAGLGVRMY
                     LHEMKNGMTPLEQYDYKFGCDSRPVIIGWMQAGLIDMLSHDDIPYLQGLIEEAGLRWN
                     "
     gene            922..2034
                     /locus_tag="ACIYSB_005544"
     CDS             922..2034
                     /locus_tag="ACIYSB_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGC5432335.1"
                     /translation="MNAHSERLELRGSQRLLLELIRLHAPVTRAELARLSGLTAGAIT
                     QQCRELMFSGLVVEGERNTGQRGQPSLPLRLNPGGGCAIGLSFSPGFIDMTVVDLSGR
                     QLLSLCEAHQENQPLEASLAQIKTLVEKMLKKRQLQHARILGIGYAVPGFLKADGRKR
                     HSVPWLESWREVDLQQAFADNLPWPTWVENNANASAVGELYSGKWNAYSDLTFIDLGY
                     GIGAGIISGNKMLRGGFCNAGEVGMAFPSGTPRPSYKDLLATLEREGFSETKLPELIA
                     GQHPLLESWFERCRGQVEQTLFSCVQWVDPQIIVLGGAMPKPIISRLVQELNQLMEAC
                     LDPNRPRVKLAASSMGAQSASCGAAMLPLYKVINNG"
BASE COUNT          466 a          537 c          631 g          452 t
ORIGIN      
        1 ataacaaaaa catctttcac atttaaattt aagtagtaaa gttaattacc atcttacatt
       61 cgagaggaaa agaagatggc gtttaacttt caccgggcgg cagaggggat cgtgctggcc
      121 aacagccacc gaggttattc cagctgcgcg ccggagaaca cattcccggc ctttgaagcc
      181 gcccgcctgg cgggaaccca ctgtattgaa atcgatatcc acctgacggc ggataaccaa
      241 ctggtggtca cgcacgatca ccgtatcgac cgggtatcca ccggacacgg atttgtggag
      301 cagatgacgt acccgcagct gctggcgtgc gactttggcg tgaaatacca tccgtcgttt
      361 gccggaacgc gcatcccctt gctggcggac gtgctgcgct gggcggtgga aaacagcatg
      421 gggctgattg ttgaggtcaa acagcgcaac cgccatgacg agtttgtggc ggcgctggtc
      481 gcactgctgc atgagcatct ggatgccctc ggccatatcc agttgctggg cttcaaccat
      541 gtgctgatca accgggtgaa ggcgcaaatc ccgcaactgg cgttacaggt ggtgacgctg
      601 gagcgctaca ataaccagct tgcggctgta cagcagtcca atgccggatg cgtctgcttt
      661 gagttcgagt tcgcccatat cgacgacctg cgggcctaca aaaatgccgg actgggcgtg
      721 cggatgtatc tgcatgaaat gaaaaacggc atgacgccgc tggagcagta cgactataaa
      781 ttcggctgtg acagtcgtcc ggtgattatc ggctggatgc aggcggggct gattgatatg
      841 ctcagccacg atgatattcc gtatttgcag ggactgattg aagaagcagg cctgcgctgg
      901 aactagaaaa ataaggacac catgaacgcc cactctgaac gccttgaact acgtggttcg
      961 cagcgcctgc tgctggaact gatccgtctc cacgccccgg tgacgcgggc tgaactggca
     1021 cggctgtcgg gactgacggc gggggcgatc acccaacagt gccgagagct gatgttctcc
     1081 ggcctggtgg tggaagggga gcgcaatacc gggcagcgcg gccagccgtc gctgccgttg
     1141 cgccttaacc ccggcggcgg ttgtgctatc gggttgtcgt tcagtcccgg ttttatcgat
     1201 atgacggtgg tcgatctcag tggtcgccag ctgctgtcgc tttgcgaagc acatcaggag
     1261 aatcagccac tggaggcctc tctcgcgcag atcaaaacct tggtggagaa aatgctgaaa
     1321 aagcgccagc tccagcacgc acgtattctc ggcattggct atgcagtgcc cggatttctg
     1381 aaagcggatg gtcgcaagcg ccacagcgta ccgtggctgg agagctggcg tgaggtagat
     1441 ctccagcagg cgtttgccga caacctgccg tggccgacgt gggtggaaaa taatgccaac
     1501 gcctcggcgg taggtgaact gtacagcggc aaatggaatg cgtacagcga tctaaccttt
     1561 atcgaccttg gctacggtat cggcgcgggt attatctccg ggaataaaat gctacgcggc
     1621 gggttctgca atgccggtga agtcgggatg gcgtttccat ccggtacacc gcgcccgtcg
     1681 tataaggatt tgcttgccac actggagcgt gaaggattca gtgaaactaa gctaccggag
     1741 ctgatagccg ggcagcaccc actgctggaa agctggtttg aacgctgtcg tggccaggtg
     1801 gagcagacgc tcttcagctg tgtgcagtgg gtagacccgc aaatcatcgt actcggtggc
     1861 gcgatgccga aaccgattat cagtcgtctg gtgcaggaac tgaatcaact gatggaagcc
     1921 tgcctcgatc ccaaccgccc cagagtgaag ttagctgcct cttcgatggg agcacagagt
     1981 gcgtcatgcg gcgcggctat gctcccgttg tataaggtga ttaataatgg ataagaagtt
     2041 atgtgattgt tgcttctgaa agtgatattt tatgttgtta aaatgt
//
LOCUS       DBBGLQ010000292         2077 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.296, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000292 DBBGLQ010000000
VERSION     DBBGLQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.296"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(12..248)
                     /gene="tnpA"
                     /locus_tag="ACIYSB_005545"
                     /pseudo
     CDS             complement(12..248)
                     /gene="tnpA"
                     /locus_tag="ACIYSB_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(338..1385)
                     /locus_tag="ACIYSB_005546"
                     /pseudo
     CDS             complement(338..1385)
                     /locus_tag="ACIYSB_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1719..>2077
                     /locus_tag="ACIYSB_005547"
     CDS             1719..>2077
                     /locus_tag="ACIYSB_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HGC5432336.1"
                     /translation="MHSIKKLTMLLGFLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDVAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEG"
BASE COUNT          494 a          542 c          461 g          580 t
ORIGIN      
        1 aatgccgtct atcccataaa gccggatacg ctcattttgg gcgggatctc cacaagcata
       61 tggatatgat ctgcacagca ctcagcttca agaatccgta cacttttcca ctcacacagc
      121 tttctcaaaa tactgcctgt tgctttacgc ttctctctgt agaacacttt tcttcggtat
      181 tttggcgcaa aaactatgtg atatttacag ttccatcggg tatgcgctaa gctcttttcg
      241 ttccccattg ggaccccctt ttgatttctt gtttgacact tgcagttgcc agaccgcaag
      301 gtgttttaac aaatcaaaag gggttttaat aactggctca aagctgaaag ctttccggcc
      361 cccagcctag ctgggggttt tctgtgcaca aaaatccgga aacgagtgag catttcagga
      421 ttcttaaaca gaccctggat cggtcaactg ctcctaaact gatcggcatt acacctgtcg
      481 gctgccgttt tttcagctcc gtgatccatg ggccgggttc tttggtcagc aatgctgccg
      541 cccttgcccc atggataaat aatgtccggt ggtaggtatc cccacgtttg cttatcccca
      601 gcatgcgaac cttcccaccg gaccccgtct gcttcggcac cagacagaca taagcgtcaa
      661 actcacgtcc ggatttaaat gctgaggcct cgcccatggt ggcgaccacc gccgtagcga
      721 ttaacggccc cacaccgggg atttccatta ggcgcttaca gttttcgctc tgccgtgcca
      781 cgctgttaat ctgtttatca aggccatcta tctgcatgcc cagctcactc agtctgttgt
      841 actggtattc aagcacagtg atgagatacg gcggcaggtt cgtcttcatt cgctctaggg
      901 ctgtcggtag ctttctgtcc atcgctgtcc gatctttgtg gaggatttca ccaaactcga
      961 ggagcgtgcc aagggtatta atatgcgagg ttctgaactt caccagttgt ctgcgcgaac
     1021 ggtgcaggac cagaactgac tgctgctctt ccgttttaac agcgatatct ttactgggct
     1081 gctgtaccgc catccagata gcacgcgcat ccatcacatc atttttgttg cccatgacga
     1141 atgctttaac gaagcgagct tgcaacagcc gaactttgtg tcccagtttt ttccagttcc
     1201 ctcgcccaat gttgggagcc tccacaagcc tccataccta tcagacaggg ttcccggttg
     1261 ctgaagaacg tcagaaaatc cttgctacgt agctgcttgt ccaccacttc gcccgtgtac
     1321 tcattgatga agtgaacctg aatcagatac tttgcgatat caacgccaac cggtgtatat
     1381 ttcatttgtg gagtcgccag tctgagggcg cttttgcatc ccgtattggg cactatgatg
     1441 ccggaaatct gagaggctcc acagcagaca attttaccct tcatccatca cagtgcacct
     1501 ctcttaacag ggcaggatgt gttcagtaca tttctccttt tatggttaca gtttatccat
     1561 catggcgtaa ttgaaggctg tcgtgaagga gaggaagagc atctcatcac ttacaaacaa
     1621 atacattaat gataaccatt tgcatttgta tagattatgc tataaaacat agccgttgct
     1681 atgtttgttc taattttgct cactataggt actgaattat gcactcaata aaaaaattaa
     1741 ccatgctctt ggggttcctc gccctcacct gctcgatagc atttcaggcg agtgcaactg
     1801 aaaaattcaa ggtcattaca acatttacca tcatcgcaga tatggccaaa aacgtggctg
     1861 gagatgttgc agaagtctca tccataacca agcccggtgc ggaaattcat gagtatcagc
     1921 ctacccctgg cgatattaaa cgtgcgcagg gggcacaact gattctcgcc aatggaatga
     1981 atctggaatt gtggttccag cgcttttacc agcatctcaa tggtgttcca gaagtgattg
     2041 tctcttcggg tgtgacgcca gtagggatca ccgaagg
//
LOCUS       DBBGLQ010000293         2074 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.297, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000293 DBBGLQ010000000
VERSION     DBBGLQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.297"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..170)
                     /locus_tag="ACIYSB_005548"
     CDS             complement(<1..170)
                     /locus_tag="ACIYSB_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGC5432337.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDIDGVELTELINKVAEYGY
                     SLRVVDERDATET"
     gene            complement(167..541)
                     /locus_tag="ACIYSB_005549"
     CDS             complement(167..541)
                     /locus_tag="ACIYSB_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGC5432338.1"
                     /translation="MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFA
                     DERVIELHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(631..999)
                     /locus_tag="ACIYSB_005550"
     CDS             complement(631..999)
                     /locus_tag="ACIYSB_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGC5432339.1"
                     /translation="MSDTLPGTTHHDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYHPDQAFPLPMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(1049..1693)
                     /locus_tag="ACIYSB_005551"
     CDS             complement(1049..1693)
                     /locus_tag="ACIYSB_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HGC5432340.1"
                     /translation="MSSIITITVYTLDELSCPVRGKARDGYRQHHADSNWYENVYEDF
                     RAVCDIFGIDLRQRVFRLSNGRFMEEPCIWFSGCCSQGDGACFEGRWHWQPATARIIR
                     KYAPQEHELHRIADALQAVQKRNFWQLQAEIHRRGRYCHPYSMDITVTRNSPTGQTMT
                     TDAEAAVSEALRDLALWLYRQLENEHNRLTSDAPVDEALLINEYTFTEAGLRTG"
     gene            complement(1712..1933)
                     /locus_tag="ACIYSB_005552"
     CDS             complement(1712..1933)
                     /locus_tag="ACIYSB_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGC5432341.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAETYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(1996..>2074)
                     /locus_tag="ACIYSB_005553"
     CDS             complement(1996..>2074)
                     /locus_tag="ACIYSB_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGC5432342.1"
                     /translation="VDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT          469 a          549 c          574 g          482 t
ORIGIN      
        1 gtttcggtag cgtcacgttc atccaccacg cggagtgaat aaccgtattc agcgaccttg
       61 ttaatcagtt cagtgagctc aacaccatca atgtcaacga caattcgccc tatattcagt
      121 gcctgtacgt taacgctgtc tgcttccaat gtcagggcca gtttcatcat ttcgcctccg
      181 gatacttacc cagggtaatg ttatttaccg ttctgtaatt gtcgcgggtc atcaggccgg
      241 tcgccctgcg agcccggagg atatcgatgc tgtttattaa ctgagagcgg gtacaggcgc
      301 tgaatcccgg ctggtcggta cgcaccagcg cgtatttttc cacgagaaag ttcaccgcat
      361 cacacagtga aatgcctgcc tcaatatgca gctcaatcac gcgttcatcc gcgaacggtg
      421 tgtcattcag cgtaaggctg taatgctggt ccagtaaccg ggtgagcagt gtctgccaga
      481 tggtgacggg agacgggcaa cacgatgcct cccgtacatg cgtgtcgggt aatgttttca
      541 taatgaagat tttcctgata tgcagagata aaaaatggga cagtggcgtg gtgaaaacac
      601 caggccggag cagaagatta ttctgatgat ttactttttc gtttcgggcg tcggataaac
      661 agccagataa acgtaaccac aggagccgag ggtgtcggct tcacaggtca gccccttcgc
      721 atacagtgtc acggtatgct gatggcgggg attcagttca ccgctggtga gcatgagctc
      781 cagttgtttc atcggcagcg gaaaggcctg gtccggatgg tacgcatccg catctctgaa
      841 ccggcctctg ataccggcac ggtcggcaag gtaatgcaac cggttaccct cctgcaccag
      901 acgtgccccg aaacagggcg tcacggtgca gggcagtccc caccagaggc ggtcgtgatt
      961 gtcgtcgtga tgcgttgtcc cggggagtgt gtctgacacg ataaaatcct cacagaaaat
     1021 cgttgaatga tagtttaact ttgacaggtc agccggtgcg cagaccggct tcggtgaagg
     1081 tgtattcgtt gatgagaagg gcctcatcca caggggcatc tgaggtcagc ctgttgtgct
     1141 cgttttccag ttgacggtac agccataagg ccaggtcgcg cagggcttcg ctgacagcgg
     1201 cttccgcatc tgttgtcata gtctgaccag tcggactgtt ccgggtaaca gtgatatcca
     1261 tgctgtacgg atggcagtag cgtccccggc ggtggatttc agcctgcagt tgccagaagt
     1321 tacgcttctg caccgcctgc agggcatccg cgatacgatg gagttcatgc tcctgtgggg
     1381 catacttccg gattatccgg gctgtcgccg gctgccagtg ccagcgtcct tcaaaacagg
     1441 ctccgtcccc ctggctgcag catccgctga accagatgca cggctcttcc ataaacctgc
     1501 cgttgctgag ccggaaaacg cgctggcgga ggtcaattcc gaaaatgtca cagaccgccc
     1561 tgaagtcctc atacacgttt tcataccagt tgctgtcagc atgatgctga cggtacccgt
     1621 cgcgggcttt tccccgtacc gggcaggaaa gttcatccag cgtatacact gtgatggtga
     1681 taatgctgct catcagaatc ccctttctga ttcagttcag ggtgacgctc atcagccgga
     1741 catacgggcc aaaactgtcc ttacggcgtt cagcaaacac ggccagcaca ccgggaatat
     1801 cctgtacttc acgaccggta tacgtctcag cactgccgtg ccagcggtac ttaccggtgc
     1861 agaacggaaa tagacgggat gcaggatgct gttggtgaat acgcatggct tcaccacggg
     1921 tgataatttt cataatggga tacctctgaa gacagaagat aaaagtgaaa acaggtgtga
     1981 tgtggttgtg acgggttaaa gcagaccatg ttcggcaaag gagaaaacct ggctgccacc
     2041 gactatcaga tggtccggca cccggatatc cacc
//
LOCUS       DBBGLQ010000294         2051 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.298, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000294 DBBGLQ010000000
VERSION     DBBGLQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.298"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(346..843)
                     /locus_tag="ACIYSB_005554"
     CDS             complement(346..843)
                     /locus_tag="ACIYSB_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HGC5432343.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
     gene            complement(840..1373)
                     /locus_tag="ACIYSB_005555"
     CDS             complement(840..1373)
                     /locus_tag="ACIYSB_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGC5432344.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(1370..1681)
                     /locus_tag="ACIYSB_005556"
     CDS             complement(1370..1681)
                     /locus_tag="ACIYSB_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGC5432345.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            complement(1686..1901)
                     /gene="essD"
                     /locus_tag="ACIYSB_005557"
     CDS             complement(1686..1901)
                     /gene="essD"
                     /locus_tag="ACIYSB_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGC5432346.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          566 a          497 c          403 g          585 t
ORIGIN      
        1 gaaattagcc cttgggcaga atggtccgaa aaaaaatatc agagagaaaa accaacaagg
       61 aaatctcaag aggtacaaat aataaaatta taacaatgac tgcttcagat aaatttgtaa
      121 caaaccagaa caccattaac gcatgattaa ccacccatag caaggattac ttttgtaaag
      181 aaaaacacag caatgaaaga atagctttat ttattaataa aacgtgtcat tctgattaag
      241 accttttatc ttacccttaa gatttcagga attttggctc atggaagagt cctttttatt
      301 taaattttac attccgcgat gtaaatgttc cgatttaata ttaccctaca tttgatgctt
      361 tttatctctt aaagattcat agatctgttg acaagtcact cctgcgatgt agcgttcgtc
      421 agcaatttca gcataaagct gagcttctgc tgcaatatct ccgagcatgt tggtgagcat
      481 tccttcggcg gttttggttg ttttgcctct gacggcagcg gcaagatctg cggtatgctt
      541 cgctgcgtca aggcgtatgg catatttttt tgcttcggca cgcaactggt taacactatc
      601 agacagataa gcagccctgg cagaaatttc agcagatttc tgttgcgcat ctttaacagc
      661 ctcatcacgg gctatagttc gcccctgttc aattattcga gcagcaaatt gagcatttac
      721 ctcttgtgat aatgcggcag catcacgttc cgcccatttt ttttgccatc ctcggtcgct
      781 ccagacattt ccgacgataa atcctgacaa cacgagaaaa atcaccatga atatctgatt
      841 cactgttcta tcccccagca ggttaatgcg ctctcctggt cacgacgaat aacctgaccg
      901 taacagttat ttgaacgaat gcggcaatcg cgtccgccat ccttaatcca ccagcgaatc
      961 gcttcgcatg cacctttacg atcaccagca ttcagccgct tataaaacgt cgacgggaaa
     1021 cacttaccgg ggccaatgtt atagggacaa aatgacgcga tacccgcttt ttgtggttcg
     1081 gtcagtggta ctttaatatt gcgctccacc catgccagcg ccttatcacg ctcaatggcg
     1141 ttgacctggt cgcatttttc cttcgacagt ttcatattgg gaaaaacggt ttttccatcc
     1201 accactgtgg caccccgaca gatggtccat atgccagaac catcgcggta tgccattgtg
     1261 tggttacctt ctttttcgtc cagaaactgg tcaagtatct gaggagcaga tgcgccagca
     1321 ccaatcagcg ccagaacggc agccgacagg ccgtatctga tttttgtgtt catagatatt
     1381 tatgatgagg acgctcgtgc ttattggcag gattttcaat cttaaaggag tactgatgct
     1441 gcagataaga ctcaactttt tctgacaatt tttctgctac ttccaggaag acttgccgga
     1501 cgctccttct ggctgctgcc tcataaaact ccagcgcagc tccttcaaca cggtccatgg
     1561 cgacatccag gtcaaaaatt tcaccgtcaa agcgttcttt gtcctgtaag gctacagtta
     1621 ccgtaacttt attctcaaaa ttacggactc ctttcacaac cagttcatag tcttgagtca
     1681 ttggattact ctcctctcgc agccttacgc ctgtcttctt taatcttgaa ataaagattt
     1741 gtcagatacg tcagcaggcc aaaaaccagg ctacccagca caccgattgc agcccactgt
     1801 gacggagtta ctttatcgag taactgcaat gcccagaaac cagcattacc cgccgatgtg
     1861 ccataggcaa cacctgttgt taacttatcc attgatttca tatcctcacc ccgatgtaca
     1921 cggatggtgc aatatgtttg aaaagatcgg agtctacggg gtagttttga cagcacacgt
     1981 tgttctcaac ggcgctaaaa aaacatacac attaaaaatg tgggtaatta ttttgaaaga
     2041 aagtcatata t
//
LOCUS       DBBGLQ010000295         2048 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.299, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000295 DBBGLQ010000000
VERSION     DBBGLQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.299"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            267..920
                     /locus_tag="ACIYSB_005558"
     CDS             267..920
                     /locus_tag="ACIYSB_005558"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HGC5432347.1"
                     /translation="MLNSITYSGKRTGVLAIGAHPDDIELGCGASLATFSARNIYTIA
                     VVMTPGCCGGKDTDRHKESQKALKILGCDKSIHLNFEDTRLYLQIRDMISSLENIIAN
                     DIPQDVEITRVYTMNNSDRHQDHIAVYQASMVACRYIPQILGYETPSAWISFSPHAFE
                     AIEQNEFNKKLMALKEHKSQSQRKYMQHEYLHSQASFRGQQANCAMCEGFIIYKMVL"
     gene            934..>2048
                     /locus_tag="ACIYSB_005559"
     CDS             934..>2048
                     /locus_tag="ACIYSB_005559"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGC5432348.1"
                     /translation="MKTWIFICMSIVMLLWFLSTLRRKPSQKKGCIDVIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     HGIKYATCDQVFLSDADTFVPPDSDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIAMKRTLQQFLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQVNRCIVYPQECNSLREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVMAMFPLIWVGIVCVIGTFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPT"
BASE COUNT          555 a          407 c          479 g          607 t
ORIGIN      
        1 tatctccttc agtgtgaatt cagttccact ttacttttcc ggttcgataa ttttcctgtc
       61 acaggccagc tgacatcaga ttccatgatg cagttaagca tgagctcagt gaactggtgc
      121 taaaagtcct cactacaaga cagaatcagt gtattactga tgtcaggcat catctgagaa
      181 agttaattag ttaatttgaa attaatacaa tggtaatatt tataatgggg aaaagcatat
      241 aacaggaagt agataaggaa ggtatcttgt taaattctat cacatattca ggaaagagaa
      301 caggtgtact ggcaataggt gcacatcctg atgacataga attaggatgt ggtgcatctc
      361 ttgcaacttt ttctgccaga aatatatata ctatcgcggt agttatgacc cccggttgtt
      421 gtgggggaaa agatactgac cgtcataaag agtcacagaa agcattaaaa atactgggat
      481 gtgacaagtc aattcatctt aattttgagg atacccgtct ttatttacag ataagagaca
      541 tgatatcttc cctggaaaat ataattgcta atgatatccc tcaggatgtc gaaattacga
      601 gagtttatac catgaataat tctgacaggc atcaggatca tattgctgtt tatcaggcat
      661 caatggttgc ctgtcgttat attcctcaga tactgggata tgaaacacca agtgcatgga
      721 tttcattttc ccctcatgct tttgaagcaa tagagcagaa tgaatttaat aaaaaattaa
      781 tggcattgaa agagcataaa agccagagtc aacgtaaata tatgcaacat gaatatttac
      841 atagtcaggc cagtttcaga gggcaacagg caaactgcgc tatgtgcgaa ggttttatta
      901 tatacaaaat ggtgttatga ctggatttca taaatgaaga cttggatatt tatctgtatg
      961 tccatagtaa tgttgctatg gtttttaagt acgctaagac gtaaacccag tcaaaagaaa
     1021 ggctgtattg acgtcattat acctgcttat aacgaaggcc cgtgtctggc gcagtcactg
     1081 gataatctgc tgcgtaaccc ttatttttgc cgggtaattt gcgttaacga cggctccacg
     1141 gacaataccg aagcggtcat ggcggaagtc aaacgcaaat ggggcgaccg ctttgttgcc
     1201 gtcacgcaaa aaaacaccgg taaaggtggc gcactaatgc atggaataaa atacgccacg
     1261 tgtgatcaag tatttttaag tgatgcagat actttcgttc cacctgacag tgatggaatg
     1321 ggctatatgc tggcagaaat tgagcgcggc gctgatgccg ttggcggaat tccctccacc
     1381 gcgttaaaag gggcaggtct gctgccacat atccgcgcca ccgtaaagtt gccgatgatt
     1441 gctatgaagc gtacgctaca gcagttcctg ggtggtgcac cgtttattat cagcggtgcc
     1501 tgcgggatgt tccgtaccga tgtgctgcgt aaattcggtt tttcggaccg taccaaagtt
     1561 gaagatctcg atctcacctg gacattggtc gccaatggtt accgtattcg gcaggtgaat
     1621 cgctgtatcg tatacccaca ggaatgcaac agccttcgtg aggagtggcg tcgctggcgg
     1681 cgctggattg tgggctacgc ggtctgtatg cgcctgcata aaagactttt atttagccgc
     1741 ttcggtatct tcagtatatt tcctatgctg ctggttgtgc tttatggcgt tgggatttat
     1801 ctcactacct ggtttaatga attcatcacc accgggccac atggtgtggt gatggcaatg
     1861 ttcccgctta tctgggtcgg catagtttgt gttattggta cttttagcgc ctggtttcat
     1921 cgttgctggt tgttggtacc tttagctccg ctttccgttg tgtatgtatt attagcttat
     1981 gccatctgga ttatttatgg acttattgcc ttttttactg gacgcgaacc tcagcgcgac
     2041 aaacccac
//
LOCUS       DBBGLQ010000296         1992 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.300, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000296 DBBGLQ010000000
VERSION     DBBGLQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.300"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..190
                     /locus_tag="ACIYSB_005560"
     CDS             <1..190
                     /locus_tag="ACIYSB_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432349.1"
                     /translation="PRTQTTDNGKQNTYFSSLDNMVAQGNALPVLYGEMRVGSRVVSQ
                     EISTADEGDGGQVVVIGR"
     gene            251..>1992
                     /locus_tag="ACIYSB_005561"
     CDS             251..>1992
                     /locus_tag="ACIYSB_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGC5432350.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDVISEGPIQGPVDGLKS
                     VLLNGTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGRWLTEKDITIKGKTT
                     SQYLAPVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSDNPAWCL
                     WDMLTHPRYGMGKRVGAADVDKWALYVIGQYCDQSVPDGSGGTEPRITCNAWLTRQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWSDPDNGQETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLTVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIREND"
BASE COUNT          440 a          515 c          656 g          381 t
ORIGIN      
        1 tccccgcaca cagacaacgg ataacggcaa acagaacacg tatttctcct cactggataa
       61 catggttgcc caggggaatg ctttgcccgt tctgtacggt gaaatgcgcg tggggtcacg
      121 ggtggtttct caggagatca gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat
      181 tggtcgctga tgcaaaacat tttatgtgaa accgcctccg ggcggttttg tcgtttatgg
      241 agcgtgagga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
      301 tctgaaatcc acgcagttgc tgagtgtgat cgatgtcatc agcgaagggc cgatacaagg
      361 accggtggat ggattaaaaa gcgtgctgct gaacggtacg ccggtgctgg acagtgaggg
      421 gaataccaat atctccggcg tcacggtggt gttccgggcc ggtgagcagg agcagacacc
      481 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaagtacga
      541 cacgccgatc acccgcacca tcacgtcggc aaacatcgac cgtctgcgcc tgaccttcgg
      601 tgtgcaggca ctggtggaaa ccacctcaaa gggggaccgg aatccgtcgg aagtccgcct
      661 gctggttcag atacagcgta acggtcgctg gttaacggaa aaagacatca ccattaaggg
      721 caaaaccacc tcgcagtatc tggccccggt ggtggtgggt aacctgccgc cgcgcccgtt
      781 taatatccgg atgcgcagga tgacgccgga cagcaccaca gaccagctgc agaacaaaac
      841 gctctggtcg tcatacaccg aaattatcga tgtgaaacag tgctacccga acacggcact
      901 ggtcggcgtg caggtggact cggagcagtt cggcagccag caggtgagcc gtaattatca
      961 tctgcgcggg cgtattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag
     1021 cggtatctgg gacggaacgt ttaaaccggc atacagcgat aacccggcct ggtgtctgtg
     1081 ggatatgctg acccacccgc gctacggcat ggggaaacgt gttggcgcag cggatgtgga
     1141 caaatgggcg ctgtatgtta tcggccagta ctgcgaccag tcggtgccgg atggctctgg
     1201 tggcacggag ccgcgcatca cctgtaatgc ctggctgacc aggcagcgta aggcgtggga
     1261 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
     1321 gacgttcgtg caggaccggc cgtcggataa ggtgtggacc tataaccgca gtaatgtggt
     1381 gatgccggat gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa
     1441 tgccgttgag gtgaactgga gtgacccgga taacggccag gagacggcga cagagcttgt
     1501 ggaggacacg caggccattg cccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
     1561 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cagaactgct
     1621 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgccatg taccgggcga
     1681 tgtcattgaa atctgtgatg atgactatgc cggtatcagc accggtggtc gtgtgctgac
     1741 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
     1801 taccacgctg ataagtctgg ttgacggaag tggcaatccg gtcagcgtgg aggttcagtc
     1861 cgtcaccgac ggcgtgaagg taaaagtgag ccgggttcct gacggcgttg ccgagtacag
     1921 cgtgtggggg ctgaagctgc cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg
     1981 tgagaacgac ga
//
LOCUS       DBBGLQ010000297         1985 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.301, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000297 DBBGLQ010000000
VERSION     DBBGLQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.301"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..84
                     /locus_tag="ACIYSB_005562"
     CDS             <1..84
                     /locus_tag="ACIYSB_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGC5432351.1"
                     /translation="DAFIRIGSIAMVRVLLDAMKPVEEDDE"
     gene            445..1714
                     /locus_tag="ACIYSB_005563"
                     /pseudo
     CDS             445..1714
                     /locus_tag="ACIYSB_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178403.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1695..1985
                     /locus_tag="ACIYSB_005564"
                     /pseudo
     CDS             1695..1985
                     /locus_tag="ACIYSB_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          530 a          437 c          532 g          486 t
ORIGIN      
        1 gatgctttta tccggattgg ttctatcgcg atggtgcgag tactacttga tgcaatgaag
       61 cctgtcgagg aagatgacga gtaacagagc tcggtatctc aatcaggagc tgattgtcct
      121 ggttcgatgc cgaaaaccag tgatttttcg tgttggtatc tgtgttggta taagatgata
      181 ttgtttatca ttaacaattt taaatcatga ggttaagtca catgttcgag tccggccttc
      241 gcaccatcgg aacatcaata gacgtcaacg gacgtctttt tttgtgccta aaatccagta
      301 tccgcaaggc tttcctcgct ttttcactca acctaagtca acctgattca atctacatca
      361 acttactgat gcgggtacaa ctgcgggtat ttcccggttc gataatgttt gtacccacac
      421 agaacccttg aaaggatact catcatggct ctgagtgatg tgaaggttcg ttcggctaag
      481 cctgaagcaa aagcctataa gctgaccgac ggtgaaggca tggttttgct ggttcaccct
      541 aacggctcca aatactggcg gctacgttat cgctttggcg gtaaagagaa gatgctggca
      601 ttggggaaat accccgaagt gtcgttggcg gatgccaggg cacgccggga tgaggctcgt
      661 aagcttttag ctaatggtgt cgatcccagt gaaaacaaga aagctgttaa ggtagaacag
      721 gaacaagagg cgataacgtt tgaagtagtc gccagagact ggcacgccag caatcagaag
      781 tggtcggcat cgcatagcgc tcgtgttttg aaaagtctgg aggataatct ctttactgct
      841 atcggtaagc ggaacattgc ggaactgaag acgcgggatc ttcttgtacc cattaaagca
      901 gtcgagtcat ccgggcggct cgaagttgcc gcccgtttac agcagcgtac taccgcgatt
      961 atgcgctttg ccgttcagag cggcttaatc gactacaacc ccgcgcaaga gattgcaggt
     1021 gcggttgcta cggcgaaaag acagcaccgt gcagctcttg aactgaaccg tatacctgaa
     1081 ttacttcatc gcatagatca ctattctggc agaccgttaa ctcgactggc ggttgaactc
     1141 actttattgg ttttcatccg ttcgagcgag ctgcattttg cccgctggtc agaagtagat
     1201 tttgaaacgg ctatgtggac aatcccaggt gagcgtgagc aactggaagg agtcaagcat
     1261 tctcagcgtg gctcgaagat gcggacacca caccttgtac ctttgtcgcg gcaggcccta
     1321 agtattttgg aaaagatcaa aagcatgagc ggaaattgag agctgatttt tgtgggcgat
     1381 cacgatccac gtaagccgat gagtgagaat acggtgaaca aggctttgcg cgtaatgggg
     1441 tatgacacga aggtcgaggt ttgtgggcat ggcttcagga cgatggcttg tagttcgttg
     1501 attgagtcgg gattatggtc gagggatgcg gtagagcggc agatgagtca ccaggagcgt
     1561 agctctgtgc gagccgctta catccataag gcggaacatc ttggggagcg taggttgatg
     1621 tttgaagtgg cacactgaat ttggccacct gaacagaggt gatatgctca cctcagaaca
     1681 acacaggtgc tccaatgaaa aaaagaaatt ttagcgcaga gtttaaacgc gaatccgctc
     1741 aactggtcgt tgaccagaaa tacaccgtgg cagatgcagc cagcgctatg gatgtcggcc
     1801 tttccacaat gacgcgatgg gtgaaacaat tacgtgatga gcggcaggga aaaacaccaa
     1861 aagcctcccc cattaccccg gaacaaattg aaatccgtga gctcaggaaa aagctacaac
     1921 gtattgaaat ggaaaatgaa atattaaaaa ggctaccgcg ctcttgatgt cagactccct
     1981 gaaca
//
LOCUS       DBBGLQ010000298         1942 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.302, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000298 DBBGLQ010000000
VERSION     DBBGLQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.302"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            455..685
                     /gene="vapB"
                     /locus_tag="ACIYSB_005565"
     CDS             455..685
                     /gene="vapB"
                     /locus_tag="ACIYSB_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HGC5432352.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLR
                     PSWSSFLELEKADPGFMTEREDVVCDEGRFNL"
     gene            682..1098
                     /locus_tag="ACIYSB_005566"
     CDS             682..1098
                     /locus_tag="ACIYSB_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGC5432353.1"
                     /translation="MNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRAAVDATTEVKVALRLAGTPIGPN
                     DTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG"
     gene            1323..1793
                     /locus_tag="ACIYSB_005567"
                     /pseudo
     CDS             1323..1793
                     /locus_tag="ACIYSB_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          487 a          478 c          531 g          446 t
ORIGIN      
        1 ctaagacatc aatcatctgc tctccaatga ctagtctaaa aactagtatt aagactatca
       61 cttaaataag tgatactggt tgtctggaga ttcagggggc cagtctacac gatgaggaaa
      121 tcatcatatc gctgttttcc gcccctgcgt ggcgcatcac aagtcaggga aataacacaa
      181 catatagtgt catgagcagc cgaaggaagt actacatgtt gtacttacag gaggtggtgt
      241 tgtgtggggg agaggaagat ccgtgactga tatttttcgg atcccggtga aaaaaagaga
      301 gaaacatgca gataccatgc atatccatat gtttggcatc tgcatataac aggcatacgc
      361 atatctcaca catctgcata tctataacat atacatatgt atcacatctg catatactaa
      421 acatatataa aagccgtctt gccaggagga agacatgaga accgtatcta ttttcaaaaa
      481 tggcaataac cgcgcgatcc gtctgccccg cgatctggat tttgaggggg tgagtgagct
      541 ggagattgta cgggaagggg acagaatcat tctgcgtccc ctccgcccga gctggagctc
      601 attcctggag ctagaaaagg ccgatccggg ctttatgacg gaacgcgagg acgttgtctg
      661 cgacgaagga cgatttaacc tgtgaacaag atctatatgc tcgacacgaa catctgctcg
      721 ttcatcatgc gcgagcagcc tgaagcagtg cttaagaatc tggagcaggc ggtgctgcgc
      781 ggtcaccgta tagtggtctc ggccatcacc tactccgaga tgcgcttcgg cgccaccggt
      841 ccaaaggcat cgccacgcca cgtgcagctg gttgacgcgt tctgcgcccg tctcgatgcc
      901 atcctgccct gggatcgcgc tgcggtggat gcaaccacgg aggttaaggt ggcgctgcgc
      961 ctcgccggca cgccgatcgg tccgaacgac acggcgatcg ccgggcacgc catcgccacc
     1021 ggggcaatac tggtgacgaa taatgtgaga gagtttgagc gggtgcctgg actggtactg
     1081 gaagactggg caggataaag gagtaaggcc gcccccgacg gccttaccac tattttgcga
     1141 tgtcagtcgt attcattttc agcgttaaaa aagggctcta tacgaatacg tgtatcagcg
     1201 tgagtaagtt tcggcggcct gataaatcca ctatttccca gctggacagc cagttgccac
     1261 atggcatcac tacggggaat gctacgataa ttcctctctg tgaggaaagc ggcataatcc
     1321 tgatgcccga actgtacgcc attgagcacg aaatacgcgg gttgccggtg tctgaacgcc
     1381 tggcggtcag gcaaatgcag agtaaaccgc tactgacttc cctgtataag ctgatgcagg
     1441 agaaagaaca cacgttatcg aaaaaatgcc gtctgagaga tgcgttccgg tatatcagga
     1501 agcactgggt tgcgttgtgc aacttctgtg atgacggtct ggcggaggcg gacaataaca
     1561 cagcggaaag agcgcttcgt gcagtctgtc tcggaaagaa aaattacgtg ttcttcggta
     1621 gcgatcacgg cggcgagcgt ggtgcactgc tgtacgggct gatcggcacc tgccgtctga
     1681 acggtatcga tccggaagcg tatctgcgcc atattctgag cgtactgccg gaatggcttt
     1741 ccaaccgagt tgacgaactc ctgccatgga acgtagtact caccaataaa taagcgtcaa
     1801 tacggtgctc cgttgacgct tacgttgctt tacagcgtga tgcgttgatt gctgccggag
     1861 tggatccccg gcatttacat caggatcatg cttcaggtgc tcgtgataat cgcccgggac
     1921 ttaagtcctg tctcgctgaa ct
//
LOCUS       DBBGLQ010000299         1937 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.303, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000299 DBBGLQ010000000
VERSION     DBBGLQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.303"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            3..350
                     /gene="tnpB"
                     /locus_tag="ACIYSB_005568"
     CDS             3..350
                     /gene="tnpB"
                     /locus_tag="ACIYSB_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGC5432354.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            400..>1937
                     /locus_tag="ACIYSB_005569"
     CDS             400..>1937
                     /locus_tag="ACIYSB_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGC5432355.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRIRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLMPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          427 a          535 c          590 g          385 t
ORIGIN      
        1 cgatgatccc tttaccttcc gggaccaaaa tttggctggt tgccggtatc accgatatga
       61 gaaatggctt caacggcctg gctgcgaaag tacagacggc gctgaaagac gatcccatgt
      121 ccggccatgt tttcattttc cggggccgca gcggcagtca ggttaaactg ctgtggtcca
      181 ccggtgacgg actgtgcctc ctgaccaaac ggctggagcg tgggcgcttc gcctggccgt
      241 cagcccgtga tggcaaagtg ttccttacac aggcgcagct ggcgatgctg ctggaaggta
      301 tcgactggcg acagcctaag cggctgctga cctccctgac catgctgtaa atctctttat
      361 cctggttgtc acagaataag cccggtaaaa tacgggctta tgaacgacat ctcttctgac
      421 gacatcttcc tgctgaaaca gcgcctggcc gaacaggaag cgctgatcca cgccctgcag
      481 gaaaagctga gcaaccggga gcgcgaaata gaccatctgc aggcgcagct ggataaactc
      541 cgccggatga acttcggcag tcgttccgaa aaagtctccc gccgtatcgc acaaatggaa
      601 gccgatctga accggcttca gaaagagagc gatacgctga ctggtagggt gtatgacccg
      661 gcagtacagc gtccgttgcg tcagacccgc atccgtaagc cgttccctga atcactaccc
      721 cgtgacgaaa agcgactgtt gcctgcggcg ccgtgctgcc cgaactgcgg cggttcactg
      781 agctatctgg gcgaggatac cgccgaacag ctggagttga tgcgtagcgc cttccgggtt
      841 atccggacgg tacgggaaaa acatgcctgt actcagtgcg atgccatcgt gcaggcacct
      901 gcaccttcgc ggcccatcga gcggggtatc gccggaccgg ggctgctggc ccgcgtgctg
      961 acctcgaagt atgcagagca caccccgctg tatcgccagt cagaaatata cggccggcaa
     1021 ggtgtggagc tgagccgttc actgctgtcg ggctgggtgg atgcatgctg ccggctgctg
     1081 tctccgctgg aagaggcgct tcatggctat gtcatgactg acggcaaact ccatgccgat
     1141 gataccccgg tccaggtact gatgccgggt aataagaaga cgaagaccgg gcggttgtgg
     1201 gcgtatgttc gtgatgaccg caatgccggg tcagcgttgg cacctgcagt gtggttcgct
     1261 tacagcccgg acagaaaagg catccatccg cagactcatc ttgcttgctt cagcggtgtg
     1321 ctgcaagcgg atgcgtacgc cgggttcaac gagctgtatc gcaatggtgg gataacggaa
     1381 gctgcctgct gggctcatgc ccgccgaaag atccacgatg tgcacgtccg catcccgtca
     1441 gcactgacgg aagaagccct ggagcagatc ggtcagttgt acgccataga ggcggatata
     1501 aggggaatgc cggcagagca gcggcttgct gaacgtcagc gaaaaacgaa accgctgttg
     1561 aaatccctgg aaagctggtt gcgtgaaaag atgaagaccc tgtcgcgaca ctcagagttg
     1621 gcgaaggcgt tcgcgtacgc acttaaccag tggccggcac tgacgtacta tgcgaacgat
     1681 ggctgggtgg aaatcgacaa caacatcgct gaaaatgccc tgcgggcggt aagtctgggt
     1741 cgtaaaaact tcctgttctt cggctctggc catggtggtg agcggggagc gctactgtac
     1801 agcctgatag ggacgtgcaa actgaatgac gtggatccag aaagctacct tcgccatgtg
     1861 cttggcgtca tagcagactg gccggtcaac cgggtcagcg aactgcttcc gtggcgcata
     1921 gcactgccag ctgaata
//
LOCUS       DBBGLQ010000300         1927 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.304, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000300 DBBGLQ010000000
VERSION     DBBGLQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.304"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..952
                     /locus_tag="ACIYSB_005570"
     CDS             <1..952
                     /locus_tag="ACIYSB_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432356.1"
                     /translation="SKQVRKSLHNGVLRSREWLNTQELLRRKNISVTGDSFKSYIKDI
                     VRLYCYKFLYSREYLNENAYKKQYQRVINPREFNVPILQLEVAWINNNCRSSDSSSDI
                     IILPRMIRRMVVFNDTKINKQLEEMLGGIARKKVAVRNKGGLYTRIKKENDFRSMDRF
                     MVEIENIIRPEIFPTSIYPPFSLSFIERELPLLNLAHSQYKRAISKKVANSIFYTLRK
                     THVALWLHLEMFALVAFIKLYTGKGFEFMKNMCVFIDYDKGIIKSTHEELLSEIKCNI
                     QLNLKVNPDNFPELIEIYNSCFSKNILLYDVQDDGFKWCL"
BASE COUNT          638 a          297 c          362 g          630 t
ORIGIN      
        1 ttcaaagcaa gtaagaaaaa gtcttcataa tggagtactc cggtcacggg aatggctcaa
       61 tacacaggag ttactgagaa gaaaaaatat atctgttaca ggtgactcat ttaaatctta
      121 cataaaggat attgtcagat tatattgcta taagtttctc tacagcagag aatatcttaa
      181 tgaaaacgca tacaaaaaac aatatcagag agttattaac cccagagaat ttaatgttcc
      241 gatacttcaa cttgaagttg catggatcaa taataattgc cgttcaagtg attcaagctc
      301 tgatattatc atccttccca ggatgataag aagaatggtt gtatttaatg atacgaagat
      361 aaataaacaa ttagaggaaa tgttgggggg gatagcacga aaaaaagtcg cagtaaggaa
      421 taaaggcggt ctctatacca gaataaaaaa agaaaatgat tttcgttcta tggatagatt
      481 tatggtggaa atcgaaaata tcataagacc tgaaatattt ccgacatcaa tttacccgcc
      541 tttttcactt tcatttatcg agagagaatt gccactactg aatctggcgc actcacagta
      601 caaaagagca ataagtaaaa aagtggcgaa ttcaattttt tatactttgc gaaaaactca
      661 cgtggcatta tggctgcatc ttgaaatgtt tgctcttgtg gcattcatca aactgtatac
      721 aggaaaaggg tttgaattca tgaaaaacat gtgtgtattt atagattatg ataaagggat
      781 aataaaatcc acgcatgaag aactgctgag tgaaattaag tgtaacattc agttgaattt
      841 aaaggtgaat cctgataatt ttcctgaatt aatagaaata tacaattcat gctttagtaa
      901 aaatatattg ttgtatgatg ttcaggatga tggttttaaa tggtgtttat gatgtgaaaa
      961 tattaaattt atattacatt ttaattatcg acaaaaaaca cataaagaaa tctaaaggga
     1021 atgagcgtca aatgagctga tatagcgcca gtttggtatg gtcatgtgat ttgatgatta
     1081 aagcttacgg ataataacac tcctctgtga gccatataaa tgagcatgcc ataatcgtca
     1141 gttcagagat aaaactgact gaatgatctt atgttatagt aagcagtgtt taccggatac
     1201 gatgacttat ctctctcaca tcatatggat tcactttcat ggggtaaagc gatgggacta
     1261 cagacgctga ttatgatggt ttgctgtaaa gttagcacaa gtttttgctg tacagaatac
     1321 ggagtaagaa aaataattcc agagtaaatc tgctctgctt ttgaacagaa aagattcatg
     1381 cttaccgtga aaaactgaaa ccaacattca gtctgcatgg atgtcttaag atgttgagaa
     1441 tgtcggatat ggtattatat acaccttttc cattaggtcc ttttatgttt tcattaagtc
     1501 gttttgtatg tattccttca gtgattcatt agtgcatgtt tacgcattta cacacttttc
     1561 ccattaatct cctttaaatg tgtttcatta cgcattgctg tacacatttt tgaactgata
     1621 cacatccaag ttagtaaacc tgtcagttct gattttacgt taactaaatc agtttaatgc
     1681 tgctgtaaaa caatccttcc tgtttaagca atcaatctgc tattcgattt tactgatgtg
     1741 acgtttaacc tttcctgagc tttggttgtt tgtggttttt ggtttgtgtt tcgacgttaa
     1801 gctgagaaaa gtatctttac gctaaatcaa cccgaacttt cctgaggtga tctgacgttc
     1861 ttttcgatgt gcttacgacg attctgttaa acgactttct ggatgttttg ataacaatca
     1921 gattgag
//
LOCUS       DBBGLQ010000301         1921 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.305, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000301 DBBGLQ010000000
VERSION     DBBGLQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.305"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..270
                     /locus_tag="ACIYSB_005571"
     CDS             <1..270
                     /locus_tag="ACIYSB_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGC5432357.1"
                     /translation="HITETDNAWLYSLSHQTNDTGESEWIHFTGSGYLLRTDAWSYPV
                     LRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPDLPTFDW"
     gene            282..479
                     /locus_tag="ACIYSB_005572"
     CDS             282..479
                     /locus_tag="ACIYSB_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGC5432358.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IERAREDVRTLRHLQLLHQNR"
     gene            564..1430
                     /locus_tag="ACIYSB_005573"
     CDS             564..1430
                     /locus_tag="ACIYSB_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGC5432359.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LTEISVLTESIGGKTARDWAMKQDFRCGCWLMDKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYENNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            1502..1765
                     /locus_tag="ACIYSB_005574"
     CDS             1502..1765
                     /locus_tag="ACIYSB_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGC5432360.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            1762..>1921
                     /locus_tag="ACIYSB_005575"
     CDS             1762..>1921
                     /locus_tag="ACIYSB_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGC5432361.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERP"
BASE COUNT          559 a          456 c          464 g          442 t
ORIGIN      
        1 catatcacgg aaacggacaa tgcctggttg tactcgctgt cacatcagac gaatgacacc
       61 ggcgaatcag aatggattca ttttacaggt agcggatatc tgttacgtac cgatgcgtgg
      121 tcatacccgg ttctgcgact taaacgcctg ggactgtcaa aaacgttccg ccgtctggtt
      181 gtcactctta tccggcgtta tggcgtcagt ctcattcatc tggatgccag cgctgaatgc
      241 ctgcctgacc tgcccacttt cgactggtaa ccaggaacaa catgaaatca ttaaccacgg
      301 aaacagcact ggatattctg attgtgtggc tgcaggacaa tatcgactgc gaatcgggaa
      361 ttatctttga caacgatgag gataaaacgg attcggcagc actgttgccc tgtatcgaac
      421 gggcccggga ggatgtccgt actcttcgtc atctgcagct tctgcaccag aaccggtaaa
      481 tattactcac cagacttcat tccactgacg ccagcctgaa cagggctggc gttttcattt
      541 atctgcaaaa aggaatatcg attatgtctg atatcacagc ctcccgtccg gaagtggtca
      601 acgggcatac ggacgttatc tgctcaacca gtatccgcca cattctggct gtacgaaaga
      661 gtacactgct gcaaatcgac acacttatcc ggcaactgac tgaaatctca gttctgacgg
      721 aaagcattgg aggtaaaacc gcccgggact gggccatgaa acaggatttt cgctgtggct
      781 gctggctgat ggataaaccg gaaacagcaa tgaaagccat cacccgcaat ctcgatcgcg
      841 aaatctggcg tgacctgatg caacgttccg ggatgctgtc attaatggat gcgcaggccc
      901 gtgatacatg gtaccggtca ctggaatacg ataattttcc ggaaatcagt gaagcgaaca
      961 ttctgagcac atttgaacaa ctgcaccaga acaaagatga agtgtttgag cgtggggtga
     1021 tcaatgtttt caggggactg agctggaatt acgaaaacaa ttcaccctgt aaatttggca
     1081 gtaaaattat cgtcaacaac ctggtgaggt gggaccggtg ggggtttcat cttatcaccg
     1141 ggcaacaggc agatcgactt gccgacctgg aaagaatgct gtatctgttc agcggcaaac
     1201 cgatccccga caaccgggaa aacatcacca ttcatctgga tgaccacatc cagtctgttc
     1261 agggtaaaga ggactatgaa gatgagatgt tcagcatcag atactttaag aagggctccg
     1321 cgcacatcac gttcaggaaa ccagaactgg ttgacaggct taatgatatt attgccagac
     1381 actacccgga agccctggca agcacagtga acaaaagccg caaggcataa aagaggtatt
     1441 ttacaattcg cgaatattaa ctattattaa ttgatagtaa tcaatcggcg agaaaatcac
     1501 catgcctacc agtgtatccc tcagtcctta ctttgaaacg tttatccgcg aacaaataga
     1561 aagtggtcgg tataacaata caagtgaagt cattcgtgcc ggacttcgtg ctctggaaga
     1621 acgggaacag cagataaagc ttgaatcact ccagagcgcc gttaccgcag gcatcaacag
     1681 cggtgaaagt aaaagtgctg aagaggtatt cgggcgactg acgcacaaat acaaaaagat
     1741 ggcagaaggt gaacagccaa tatgaaactg actgtttcac cattagcaga acacgatctt
     1801 gaagctatag gtgactggat cgctcaggat aatcctgtcc gtgcaatcag ttttaccgaa
     1861 gagctttacc aacaatgtct gttgatagcc gaatcgccag tactctacag agaaagaccc
     1921 g
//
LOCUS       DBBGLQ010000302         1911 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.306, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000302 DBBGLQ010000000
VERSION     DBBGLQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.306"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..474
                     /gene="pinE"
                     /locus_tag="ACIYSB_005576"
     CDS             <1..474
                     /gene="pinE"
                     /locus_tag="ACIYSB_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HGC5432362.1"
                     /translation="ELIFEDKISGTKSERPGLKKLLRTLSAGDTLVVWKLDRLGRSMR
                     HLVVLVEELRERGINFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLEA
                     ARAQGRIGGRRPKLTPEQWEQAGRLIASGVPRQKVAIIYDVGVSTLYKRFPAGDK"
     gene            581..1414
                     /gene="mcrA"
                     /locus_tag="ACIYSB_005577"
     CDS             581..1414
                     /gene="mcrA"
                     /locus_tag="ACIYSB_005577"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific endonuclease McrA"
                     /protein_id="HGC5432363.1"
                     /translation="MHVFDNNGIELKAECSIGEEDGVYGLILESWGPGDRNKDYNIAL
                     DYIIERLVDSGVSQVVVYLASSSVRKHMHSLDERKIHPGEYFSLIGNSPRDIRLKMCG
                     YQAYFSRTGRKEIPSGNRTKRILINVPGIYSDSFWASIIRGELSELSQPTDDESLLNM
                     RVSKLIKKTLSQPEGSRKPVEVERLQKVYVRDPMVKAWILQQSKGICENCGKNAPFYL
                     NDGNPYLEVHHVIPLSSGGADTTDNCVALCPNCHRELHYSKNAKELIEMLYVNINRLQ
                     K"
     gene            1644..1811
                     /locus_tag="ACIYSB_005578"
                     /pseudo
     CDS             1644..1811
                     /locus_tag="ACIYSB_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
BASE COUNT          603 a          309 c          448 g          551 t
ORIGIN      
        1 gagctgattt ttgaagacaa gataagcggt acaaagtccg aaaggccggg actgaaaaaa
       61 ctgctcagga cattatcggc aggtgatacg ctggtggtct ggaaactgga ccgactgggg
      121 cgcagtatgc ggcatcttgt cgtgctggtg gaggagttgc gcgaacgagg catcaacttt
      181 cgtagtctga cggattcaat tgataccagt accccaatgg ggcgcttttt ctttcatgtg
      241 atgggtgccc tggctgaaat ggagcgtgaa ctgattgttg aaagaacaaa agctggactg
      301 gaagctgctc gcgcacaggg acgaattggt ggacgtcgtc ccaaacttac accagaacaa
      361 tgggaacagg ccggacgatt aattgcatca ggagttcctc gccagaaggt ggcgattatc
      421 tatgatgttg gtgtgtcaac tttgtataag aggtttcctg caggggataa ataaagttaa
      481 agacactttg tgtacaaaag aaagtaaaac aacagcaact tgttgcaatt ttatcaataa
      541 aagtagtatt gtcgtgaaaa attgattaaa gattaatatt atgcatgttt ttgataacaa
      601 tggaattgaa ctgaaagctg agtgttcgat aggtgaagag gatggtgttt atggtctaat
      661 ccttgagtcg tgggggccgg gtgacagaaa caaagattac aatatcgctc ttgattatat
      721 cattgaacgg ttggttgatt ctggtgtatc ccaagtcgta gtatatctgg cgtcatcatc
      781 agtcagaaaa catatgcatt ctttggatga aagaaaaatc catcctggtg aatatttttc
      841 tttgattggt aatagccccc gcgatatacg cttgaagatg tgtggttatc aggcttattt
      901 tagtcgtacg gggagaaagg aaattccttc cggcaataga acgaaacgaa tattgataaa
      961 tgttccaggt atttatagtg acagtttttg ggcgtctata atacgtggag aactatcaga
     1021 gctttcacag cctacagatg atgaatcgct tctgaatatg agggttagta aattaattaa
     1081 gaaaacgttg agtcaacccg agggctccag gaaaccagtt gaggtagaaa gactacaaaa
     1141 agtttatgtc cgagacccga tggtaaaagc ttggatttta cagcaaagta aaggtatatg
     1201 tgaaaactgt ggtaaaaatg ctccgtttta tttaaatgat ggaaacccat atttggaagt
     1261 gcatcatgta attcccctgt cttcaggtgg tgctgataca acagataact gtgttgccct
     1321 ttgtccgaat tgccatagag aattgcacta tagtaaaaat gcaaaagaac taatcgagat
     1381 gctttacgtt aatataaacc gattacagaa ataaaattat ttattaaagt cacatttaag
     1441 acgtaatacc ctacagggta aaaatttttc tgatcttaac ttctgcaaat gttaactgct
     1501 atttttatgc taaaaatggt tatcaaaact caaaaacaca tgtttataat caatgagtta
     1561 tagaaatgct aagggctaat gagttatatg caaattagta aaattatgtt gctatgtcaa
     1621 atagttacga tttagtcatc taactaatgc tgcgccatat gggttggact gaagcggctg
     1681 acctgattgt taaaggtatg gaaggcgcaa tcaatgccaa gaccgtaact tatgacttcg
     1741 aacgtctgat ggaaggcgct aagctgctga aatgttcaga gtttggtgaa gcgatcatcg
     1801 aaaacatgta atctctccat gtgttaaata ttgaaacggg cgtataacac gcccgttgtt
     1861 ttatttatgt ggatattatt aatagcatat cgagcatatt tatatgaagc c
//
LOCUS       DBBGLQ010000303         1898 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.308, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000303 DBBGLQ010000000
VERSION     DBBGLQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.308"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(1..668)
                     /locus_tag="ACIYSB_005579"
                     /pseudo
     CDS             complement(1..668)
                     /locus_tag="ACIYSB_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(671..1039)
                     /locus_tag="ACIYSB_005580"
     CDS             complement(671..1039)
                     /locus_tag="ACIYSB_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbC/YhhH family protein"
                     /protein_id="HGC5432364.1"
                     /translation="MKYSSIFSMLSFFILFACNETAVYGSDENIIFMRYVEKLHLDKY
                     SVKNTVKTETMAIQLAEIYVRYRYGERIAEEEKPYLITELPDSWVVEGAKLPYEVAGG
                     VFIIEINKKNGCVLNFLHSK"
     gene            complement(1079..>1898)
                     /locus_tag="ACIYSB_005581"
     CDS             complement(1079..>1898)
                     /locus_tag="ACIYSB_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HGC5432365.1"
                     /translation="HLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHVYQ
                     PYRLPGQQHDEESGLYYNRHRYYDPLQGRYITQDPMGLKGGWNLYQYPLNPLQQIDPM
                     GLLQTWDDARSGACTGGVCGVLSRIIGPSKFDSTADAALDALKETQNRSLCNDMEYSG
                     IVCKDTNGKYFASKAETDNLRKESYPLKRKCPTGTDRVAAYHTHGADSHGDYVDEFFS
                     SSDKNLVRSKDNNLEAFYLATPDGRFEALNNKGEYIFIRNSVPGLSSVCIPYHD"
BASE COUNT          527 a          477 c          299 g          595 t
ORIGIN      
        1 tttttatgct ctggattaat ataatcacta catctatctg agcaatctgt tgttgatgga
       61 catgtcaacc catggtcatt tacagccaag tcttttttat tatcttcaat catttcagaa
      121 tgggatagct ttacttttct gccgtacata ccgaacagat ttaacccgta atctctaatc
      181 tctttttcat aacccagccc tttcgccgat cggctaacac cagcgtcatt taatttagac
      241 actcgacaaa atgccataca atggaaaaat tgatcagtcc cacctaaatt taatctcttc
      301 atatcttcat aagtatcaga taatatatcc catgctcttt gatggtttag ttgatctttt
      361 cttcttatta gcgctacatc tgcgggactt aaccctaatg gatcaatacc attcaccgga
      421 ttcagcggat acgcatacag actccatccc ccctccagcc ctatcgggtc ccgagtgata
      481 taccgcccct gcaacggatc gtagtaccgg ttccggttgt agtacagccc cgactcctta
      541 tcatactgct gccccggcag ccggtacggc tggtgcaggt gatgcgggtt ctcttcattc
      601 agctggttgc cccactcatc atactccccg ctccacgcaa tgtttccatc cgcatccatc
      661 agcgccagca ttatttacta tgtaggaaat tcaaaacaca tccatttttc ttattaattt
      721 ctataataaa tacaccaccc gcaacttcat aaggtaactt tgctccctca acaacccaac
      781 tatctggtag ttccgtaatt aaatatggtt tttcttcttc tgcaatccgt tcgccatagc
      841 gatacctaac atatatttca gctaattgta tcgccattgt ttcagttttt accgtatttt
      901 taacagagta tttatctaaa tgtaattttt ccacatacct cataaaaata atgttttcat
      961 cagaaccgta aacagctgtc tcattacagg caaatagtat aaaaaatgaa agcatcgaaa
     1021 atattgaact atatttcatt gaatctcctt ataggcccca ctaataaaag cactaaaatt
     1081 aatcatgata cggtatgcat actgaactca atcccgggac actatttctg ataaaaatat
     1141 attctccttt attattaagc gcctcaaatc gtccatcagg tgttgcgaga taaaatgctt
     1201 caagattatt atctttactt cttacaagat ttttatcgct acttgaaaaa aattcatcaa
     1261 cataatcgcc atgactatct gcaccgtgag tatgataagc agcaactcta tctgtacctg
     1321 tgggacattt tcttttcaga ggatatgact cctttcttaa attatcagtt tctgccttag
     1381 atgcaaaata ttttccatta gtatctttac agacaatacc agagtattcc atatcattac
     1441 atagagatct attctgcgtt tctttcaaag catctaacgc agcatctgca gtactatcaa
     1501 atttacttgg tcctattata cgtgaaagaa caccacaaac tccccccgta catgctccag
     1561 atctggcatc atcccaagtc tgcaataatc ccatagggtc aatttgttgt agtggattta
     1621 aaggatactg atataaattc catccccctt tcaaccccat cgggtcctga gtaatatacc
     1681 gcccctgcaa cggatcgtag taccggtgac ggttatagta cagccctgat tcctcatcat
     1741 gctgctgccc tggcagacgg tacggctgat acacatgatg cgggttctcc tcattaagct
     1801 ggttgcccca ttcatcatat tccgcgctcc acgccgtatt gccgtcttcg ctgataagcg
     1861 ccagcggcag tccccggtgg tcgcagtgat aaagatga
//
LOCUS       DBBGLQ010000304         1878 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.309, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000304 DBBGLQ010000000
VERSION     DBBGLQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.309"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..566
                     /locus_tag="ACIYSB_005582"
     CDS             <1..566
                     /locus_tag="ACIYSB_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGC5432366.1"
                     /translation="TEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRG
                     CSAEQRLAARKTRAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSN
                     GWVEIDNNIAENALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLR
                     YVIEHIQDWPANRVRDLLPWKVDLSSQ"
     gene            604..1578
                     /locus_tag="ACIYSB_005583"
     CDS             604..1578
                     /locus_tag="ACIYSB_005583"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGC5432367.1"
                     /translation="MNGARKSQACRCIGISIRTLQRWTNGNVITTDRRSDTVNRSPRN
                     KLSEAERLRILELCNSPEFASLPPNVIVPTLADRGIYIASESSFYRVLKSANQLKKRS
                     RECQYKRPDVLRAVAPNQVWSWDISYLPSWVRGQHFYLYMIVDIYSRKIIAAEVFASE
                     SGKHAAELLQRAVWNEKCSSNNLVLHSDNGGPMRSYTLLAKMYALGVLSSYSRPRVSN
                     DNPYSESLFRTLKYCPWWPENGFRTINDARVWVNRFVNWYNFEHKHSGIKYVTPAERH
                     QGNDMKILAARKAVYQLAREQHPERWGKHLRNWDYISEVYLNPEKEAA"
     gene            1671..1715
                     /locus_tag="ACIYSB_005584"
                     /pseudo
     CDS             1671..1715
                     /locus_tag="ACIYSB_005584"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
     gene            1706..>1878
                     /locus_tag="ACIYSB_005585"
     CDS             1706..>1878
                     /locus_tag="ACIYSB_005585"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5432368.1"
                     /translation="MVRNSLRFVSWKDYKAVTRDLKAIYQAPTEEAGQQALEAFAAAW
                     DSRYPQISRSWQA"
BASE COUNT          518 a          455 c          485 g          420 t
ORIGIN      
        1 taacggaagc cgcgtgtatg gctcatgccc ggagaaaaat ccacgatgtg catgcaagag
       61 cgcccaccga catcaccacg gaagccctgc agcgtatcgg tgaactgtat gccatcgagg
      121 cagaggtccg gggctgttca gcagaacagc gtctggcggc aagaaaaacc agagccgcgc
      181 cactgatgca gtcactgtat gactggatac agcaacagat gaaaacactg tcgcgtcact
      241 cagatacggc aaaagcgttc gcatacctgc tgaaacagtg ggatgcactg aacgtgtact
      301 gcagtaatgg ctgggtggaa atcgacaaca acatcgcaga gaacgcctta cggggagtgg
      361 ccgtaggccg gaaaaactgg atgttcgcgg gttccgacag cggtggtgaa catgcggcgg
      421 tgttgtactc gctgatcggc acatgccgtc tgaacaatgt ggagccagaa aagtggctgc
      481 gctacgtcat tgaacatatc caggactggc cggcaaaccg ggtacgcgat ctgttgccct
      541 ggaaagttga tctgagctct cagtaaatat caatacggtt ctgacgagcc gcttacggcc
      601 gttatcaacg gcgcccggaa aagtcaggct tgtaggtgta tcggtatttc tattcggaca
      661 cttcagcgct ggactaatgg caacgtcatc accacggacc gaagatccga tactgtaaac
      721 agatctcccc gaaataagct gtcagaagcg gaaagattgc ggatactgga gttgtgcaac
      781 tctccagagt ttgcatcctt acctccaaat gtgatcgtac caactctggc agacaggggt
      841 atttatattg cttctgaatc aagcttttat cgtgtactga aatcagccaa ccagttgaag
      901 aaacgctccc gtgaatgtca gtataaacgg cctgatgtac tcagggccgt tgcaccgaat
      961 caagtctgga gttgggacat cagctatttg ccgtcctggg tcagaggtca gcatttttac
     1021 ctctatatga tcgtcgatat ttacagtcgc aagattattg ctgcagaggt atttgccagc
     1081 gaatctggaa aacatgcagc agaactactc cagcgggcag tatggaatga aaaatgttca
     1141 tccaacaatc tggtactcca ttctgataat ggtggtccca tgcgcagtta cacgttgttg
     1201 gcgaaaatgt atgcgcttgg tgtgctaagt tcttattccc ggccaagagt cagcaatgat
     1261 aatccttact cagaatcttt gttccggact ctcaaatatt gtccctggtg gcctgagaac
     1321 ggttttcgca ctattaatga tgccagagta tgggtaaacc gttttgtaaa ctggtacaac
     1381 tttgaacata agcacagcgg gatcaaatac gtcacgccag ccgagcgtca tcaaggtaat
     1441 gatatgaaaa tactggcggc cagaaaagca gtataccagc tagcacgcga acagcatcct
     1501 gaacgttggg gaaagcatct acgtaactgg gactacatat cagaagttta cctgaatcca
     1561 gaaaaagagg ctgcataacg taattttatt acaaaaggcg acaactacct tgaaaaacgc
     1621 cgccaaactg cgatcgattc agatgccatt ttgcaccatc aacacagtat atccgaaggc
     1681 ccgcatccag ttatgcgtcg tgcatatggt gcgcaacagc ctgcgcttcg tgtcatggaa
     1741 ggactacaag gccgtcactc gcgacctgaa agcgatttat caagctccca cggaagaggc
     1801 aggtcagcag gcactggaag cgttcgctgc ggcctgggac agtcgctatc ctcagataag
     1861 ccgaagctgg caggctaa
//
LOCUS       DBBGLQ010000305         1876 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.310, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000305 DBBGLQ010000000
VERSION     DBBGLQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.310"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..291
                     /locus_tag="ACIYSB_005586"
     CDS             <1..291
                     /locus_tag="ACIYSB_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGC5432369.1"
                     /translation="KNKSGKIISTDYGIMQINSVHIPELKKLGVIKNKDELLKKPCLN
                     IHTGAWILARHFQRCGVNWECLGSYNAGFSKNNTARRMIYARIIHNRYFRKD"
     gene            304..954
                     /locus_tag="ACIYSB_005587"
     CDS             304..954
                     /locus_tag="ACIYSB_005587"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HGC5432370.1"
                     /translation="MSELLPICIILIVLAAILYLSYSAATVYLSEVHNTRPHNAAVCI
                     CCLLAVLSICLNGAYGVDATGLLFLSILTGLLTVMTLTDIAVCRLPRIFTLSLIILGA
                     AFRYSQDSLAHALLNASLWFGMIYLLRRIFLAAKGTEALGLGDVFLIAGIAMWTQPQH
                     TPLIITAAASGAFLFILIFCRRRRQLALPFAPFLCASLYVLTLFPDSVIHTSEIFT"
     gene            951..>1876
                     /gene="pilV"
                     /locus_tag="ACIYSB_005588"
     CDS             951..>1876
                     /gene="pilV"
                     /locus_tag="ACIYSB_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HGC5432371.1"
                     /translation="MKKFRHDDDKGFILLEILAGLIVIGIATPMIYSEIEDWLNEQLY
                     QSAALHADEYNNAIKNYVADKTSSLSSKTITVNELIQQGYLNNGFSRSPFGHSYITGI
                     RKNNLSGRLEALTCTTGGQDIKEDGLRRIAGQINGLGGFMLQNNSVTGAFGGWTDLGS
                     NYQITCNKGHIAMRMAGKDLEESDRLYRYSVQGRPDLNRMHTGIDMNNNSITNINEAS
                     GKNARFSGDVTSNRWLHPNGGGFTMTDSQWIRAVNNKGITTEGELKGGKVSGGTVRSE
                     GRLSTGEYLQLDKTATAGTSCSPDGLVGRDSKG"
BASE COUNT          516 a          444 c          438 g          478 t
ORIGIN      
        1 aaaaacaaat ccggaaaaat catcagtacg gattacggaa taatgcagat taattcagtg
       61 catattcctg aactgaaaaa actgggcgtc attaaaaaca aggatgaact gcttaaaaag
      121 ccctgcctga atattcatac cggtgcctgg atactggccc gccattttca acgctgcggc
      181 gttaactggg aatgccttgg cagttacaac gcaggattca gcaaaaataa cacagcccgg
      241 cgcatgatat atgcccgaat aatccacaac cggtatttca gaaaagatta agctgaggta
      301 actgtgtctg aattactccc gatatgcatt atcctgattg tccttgcggc aattctgtat
      361 ctttcataca gcgccgccac agtatattta tcagaagtac acaatacccg cccacacaac
      421 gctgcggtgt gcatctgttg tcttctggct gtcctgagca tttgcctcaa cggtgcatat
      481 ggtgttgacg cgacaggact gttattcctt tccattctga cgggattatt aactgtaatg
      541 acgctgacgg atattgctgt ctgtcggctt ccccggatat tcacgctttc tcttattatt
      601 ctgggcgcag cgttcagata cagccaggac tcactggctc atgccctgct caacgcgtca
      661 ctgtggttcg gcatgatata cctgttacga cgcatttttc tggcagcgaa agggacggaa
      721 gcgcttggcc ttggtgatgt gttcctgatt gcggggatcg caatgtggac gcaaccgcaa
      781 cacactccac tcataatcac cgccgcagcc tccggagcat ttctgtttat cctcattttc
      841 tgccgtcgca gacgccagct ggcgctgccg tttgccccgt ttctctgcgc gtccctgtat
      901 gtgctgaccc tcttccctga ttccgttatc cacacatcgg agatattcac gtgaaaaaat
      961 tcagacacga tgacgataaa ggttttattc tgcttgaaat tctggcaggc ctgatagtaa
     1021 ttggtatcgc cacgccaatg atttattcag aaattgaaga ctggctgaat gaacaacttt
     1081 atcaaagtgc ggctttacat gctgatgaat ataataatgc aataaagaat tatgtggctg
     1141 ataaaaccag ctcactttcc agtaaaacca tcacggtaaa tgaattaatt cagcagggat
     1201 atctgaacaa cggtttttcc cgctccccct ttggacacag ctatattacc ggtatcagaa
     1261 aaaataattt atccggtcgt ctggaagcac ttacctgcac aaccggagga caggatatta
     1321 aagaagatgg gttacgtcgc atagcgggcc agattaatgg actcggtggt tttatgctcc
     1381 agaataattc cgttacaggt gcctttggcg gctggaccga tctgggaagt aattatcaga
     1441 ttacctgcaa caaggggcat atcgccatgc gaatggctgg aaaagatctg gaggaaagcg
     1501 atcggcttta ccgctacagc gttcagggaa gacccgattt aaacagaatg catactggca
     1561 ttgatatgaa taataacagc atcaccaata tcaatgaagc cagtggtaaa aacgccagat
     1621 tttcaggcga tgtcacatca aatcgttggt tacatccaaa tggtggcggc ttcaccatga
     1681 cggacagcca atggatacgt gctgttaaca acaaagggat cacaaccgaa ggagagctca
     1741 aaggaggaaa agtcagtggt ggaactgtcc ggtctgaggg gcgtctttcc acgggtgagt
     1801 atttacagct ggataaaaca gccaccgcag gtacttcctg ctcaccggac ggacttgtag
     1861 gccgcgacag taaagg
//
LOCUS       DBBGLQ010000306         1844 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.312, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000306 DBBGLQ010000000
VERSION     DBBGLQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.312"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(200..1753)
                     /locus_tag="ACIYSB_005589"
     CDS             complement(200..1753)
                     /locus_tag="ACIYSB_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGC5432372.1"
                     /translation="MHEYLMFALALSPLLLMVFLILKLKMPIHYSVLISLVFTAALSA
                     IFWEMPTQNLKAAIGYGALKGLWPIVIVILGAIFSYNVMQATKALDILRDILASISED
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSILAEQVNLPVYTLGGTIILQLALFNILLPFVIVCIIGGGLKAIRGVFLITL
                     ICGISTLVPQYFVAIHLGAELPAFAGSLVSLFAVAMVSRLRKGKTDPEWRIEVSHTRE
                     TTPRSAKVLFRVGSIYLFIFVFILLCSPLFPTIKGAASQLASVLHFTLADGKTMALKV
                     EWATTPGMLIIFATIIGGFIQGASARGMLEVFIKTIFQLKNSIVAIMAIVALATVMDL
                     SGIISTLAQSIVDLTGSSYVFLAPVIGALGTFVTGSDTNSNILFGKLQTIAASKLHID
                     PNWLAAANTSGATGGKMISPQSIAIAVSATKMDGQANQIMSGTMKYCCAYIVILGLKV
                     GLFYYWFMA"
BASE COUNT          517 a          469 c          457 g          401 t
ORIGIN      
        1 aaaggcgtcg aacttcttca ccatcttttt ccagaacaac ataacaacaa tcctcgattt
       61 gaaacatcgg cgctatttta gtcagcgccg acaaatcgta aagtctgttg cctcgttccg
      121 ttcccggggc ggtgtaaacc tttttggtca ctatgtcgtc aaattcgatt gccgcgaatt
      181 tgacggggtt gacaatggtt tacgccataa accagtaata aaacagaccg actttcaggc
      241 cgaggatcac aatgtaggcg cagcagtatt tcatggtgcc agacataatc tggttggcct
      301 gaccgtccat tttggtggca gaaactgcga tggcgatgct ttgcggcgaa atcattttcc
      361 cccccgtagc gccagaggta ttggccgccg ccagccagtt aggatcgata tgtaacttgc
      421 tggcggcaat ggtttgcagc ttgccgaaca gaatgttcga gttggtatcg ctgccggtca
      481 cgaacgtgcc cagcgcgcca atcaccgggg cgaggaagac gtacgagctg ccggtcagat
      541 cgacaatcga ctgggccagc gtggagataa tcccgctgag atccatcacc gtggccagcg
      601 cgacgatcgc cataattgcg acgatagagt ttttaagctg gaaaatcgtt ttgataaata
      661 cttccagcat cccacgtgcg gacgctccct gaataaaacc gccgataatg gtcgcaaaga
      721 tgatcagcat ccccggtgtg gtcgcccatt caactttcag cgccatcgtt ttgccgtcag
      781 ccaacgtgaa atgcagcacc gaagccagtt gggatgccgc gccttttatt gtcgggaaca
      841 gcggcgaaca cagcaggata aacacaaaaa taaacagata aatagagcca acccggaaca
      901 acacttttgc cgagcgcggc gtggtttcgc gggtatggct gacctcaatg cgccactcgg
      961 gatcggtttt ccctttgcgc aggcgactga ccatcgccac ggcaaacagg ctgaccaggc
     1021 taccggcaaa ggcgggtaat tccgcgccaa gatggatggc gacaaaatat tgcggaacca
     1081 gcgtggaaat gccgcaaatc aacgtgatca gaaatacgcc gcgaatggct ttcaggccac
     1141 cgccaataat gcagacaatc acaaacggca gcaggatatt aaacagcgcg agctgcaaaa
     1201 taatggttcc gcctaaggta taaaccggca gattaacctg ctctgccaga atagaaaccg
     1261 ggattccaac cgcgccaaat gcggtcggaa cggtatttgc tacgagggaa gcaatcgccg
     1321 ccttcaacgg gttgaatccc agcgcaatga gaataccgat cggaatagcg accgccgtgc
     1381 catagcccgc agcggcttca agaaatccac caaaacacca tgaaatcagc agcacctgaa
     1441 tacgcttatc ttcgctaatg ctggcgagaa tatcgcgcag aatatccagc gctttggtcg
     1501 cctgcatgac gttataactg aagatagcgc cgaggataac aattacgatc ggccatagtc
     1561 ccttcagtgc accatagcct atcgcggctt tcagattctg tgtgggcatt tcccagaata
     1621 ttgccgatag tgcagcggtg aataccaaag agatcagtac ggaataatgg atgggcattt
     1681 ttaatttcag tattaaaaat accatcagca gcaaaggaga gagtgccaga gcaaacatta
     1741 aatattcgtg cattacaatt cccgatttac ttttttagac ttaactatat ttagggggaa
     1801 ggaggctacc ttaataataa aataaaaaat gcaaactgaa tcgc
//
LOCUS       DBBGLQ010000307         1842 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.313, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000307 DBBGLQ010000000
VERSION     DBBGLQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.313"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1109..1633
                     /locus_tag="ACIYSB_005590"
     CDS             1109..1633
                     /locus_tag="ACIYSB_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HGC5432373.1"
                     /translation="MLEQRFEDFEQQKRRDHKAQDQAQNTKQKRDRLNPPDWSQVTKA
                     GWNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDL
                     ERIGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLS
                     RREDFESSLKPSKS"
     gene            1652..>1842
                     /locus_tag="ACIYSB_005591"
     CDS             1652..>1842
                     /locus_tag="ACIYSB_005591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432374.1"
                     /translation="MNTEDKLMLKVDRLLSHDALNQRNILNNWERQFLTSVLAIYRRI
                     NKYSLVGLSPKQKNATYNI"
BASE COUNT          544 a          392 c          437 g          469 t
ORIGIN      
        1 aaaaaaatat aaatttcata ctgattaaaa gtgaaatatt ttaaagagcc agcggcaggg
       61 ggacaatcgg attcaaatgt taacatttga catcggatta tctatcctgc attgctcttt
      121 tgccgcctca aaatcccttg cgtgtttttg ctccccgatc tccagaacga ccgagccgcc
      181 acggttccgg cagcgccttg agcgcaaccg gaaaccgtct ggggagaacc ccagaccccc
      241 atcgctgtaa caccgacggt ctacgaccac ctaccaaccg cgtgactgtc tgtttcgtgc
      301 aaatgggctt tgcagggggc tggcgcggtt cctggcgacc tgaaagcccc tggttagggg
      361 tatcggcgcg cctgacctcc tgagagcctc tgtaagcgtt tttcgcgcct tccatgccct
      421 ggcgggcatc taaaacccgt ttcgtgcgtc tgagagcttt tgagcgcgtt tgaggggcta
      481 tctggcgact catccccgaa agaacaacaa cgggatcacc gacaacatgc gagaggtgat
      541 cccgctcgcc gcaagtcgat gaccgagcgt agcgagcgaa tcgacgagga agcggaagag
      601 aaccggaagc cacattgagc acttacgcac tgatgcgggg tgtcggggtg aagccctgac
      661 caggtggtaa tcgtatcggc gtgcgtgcgc ggtcatacga ttacacatcc tgtcccgatt
      721 tctgaggcgt tttaacggtg aacggacggc aaaaacacga tagagctgtt gaacgtgaaa
      781 accacgaaat ctggcaaaaa tcacggctgg cgaggcttgc agaggagtaa gccagtatac
      841 actccgctat cgctacgtga ctgggtcagg gctgcgcccc gacaccccaa atgctcgata
      901 aacacgttaa accttgtgca acaaaaaagt gctttgatag atctttagag caaaaatcaa
      961 catttcgatt aatatcaatg acttatgaca ccatcatacg aaacgttatt tgcatgtgta
     1021 tacgcaaaca tgtttgcgtg tgttgtttcg tactgtacga tataaatttc gtaaggtacg
     1081 aaagattgtt gcaggtaagg atgcaagtat gctggaacag cgatttgaag actttgaaca
     1141 gcaaaaacgg cgagatcaca aggcacaaga tcaagcccaa aacactaagc aaaaacgaga
     1201 tcggttgaat ccacctgact ggtcacaagt cactaaggct ggttggaata tgttggacgc
     1261 tatatccgag aaggttccta gggcatttag gctatattct ttttttgctc aaaatataga
     1321 taaatcgtgt ggagctgttg tatgtgatca aaaattccta gctgattatt tcggggttag
     1381 tttaaggaca attcaatatt ggatcggaga tttagaacgt attggtgcaa tagtaaaaat
     1441 accagtaggt atttattatg cttatgcttt agatccttat caggtatggc gtggatataa
     1501 taaatctaaa aaatatgcag catttaacgc aaaaacatta actcaaaaag atggtgttat
     1561 tgatcgtaag ttgcggctca tgctctctag aagagaagat tttgaaagct cactcaagcc
     1621 ctctaaatca tgactagtct tggaggtcta gatgaatact gaagataagc taatgctaaa
     1681 agttgatcga ttacttagtc atgatgcgtt aaatcaacgc aatattttaa ataattggga
     1741 aagacaattt ttaacaagtg ttttagctat ctataggagg atcaataaat attctttagt
     1801 tgggctatca ccaaaacaga aaaatgcaac gtataacatt tt
//
LOCUS       DBBGLQ010000308         1837 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.314, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000308 DBBGLQ010000000
VERSION     DBBGLQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.314"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            349..697
                     /locus_tag="ACIYSB_005592"
                     /pseudo
     CDS             349..697
                     /locus_tag="ACIYSB_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            761..901
                     /locus_tag="ACIYSB_005593"
     CDS             761..901
                     /locus_tag="ACIYSB_005593"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5432375.1"
                     /translation="MIKTRRTKRTFSPEFKLEAIEQVVKYQRDVREVALALELNPDHL
                     RK"
     gene            1010..1816
                     /locus_tag="ACIYSB_005594"
     CDS             1010..1816
                     /locus_tag="ACIYSB_005594"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGC5432376.1"
                     /translation="MEKEILKQAAVLMSEIPGETVALITQLKAKWPVRVLCHLFGIHR
                     SIYYAQVKRPVNVQRIELRSRVRAVHALSRGAVGSRAISQMLRHSGVDAGRWLARRLM
                     RECGLTSRQPVKHHYRVNEDKSPPLPNLLNRQFHPAAPNRVWCGNISFIRLQDKWCYL
                     ALVVDLYSRRIIGSALSLTADAELVCRALRNARETRPRDGHQLFHSDLGVQYKSNKYR
                     KLLWRYGVMQSMRRRGNCLDNSPMERVFRSLKSEWFPKGGMVILAMRYAI"
BASE COUNT          525 a          407 c          446 g          459 t
ORIGIN      
        1 tcaacttcag actgttacag gggcggtagc cgttatctca tcagtgtatg taaactcagt
       61 taaaaatatt ttctataact tgctgttttt atttaattca tcacaacggc cttctgcata
      121 ttaaaatcaa tactatcgct caaaacacgc cagatgattc ttgtcagctt attcgccagc
      181 gcaccagtgg ttttcaggaa ccctcgacgg gcctccatcc tttgcagaca aatactcaga
      241 ctgtcagtga gctttttcac acagcgcatt acagactgaa atggcacact gaatttggtc
      301 acctgaacag aggtgatatg ctcacctcag aacaagactg gtgccctaat gaaaaaaaga
      361 aattttagcg cagagtttaa acgcgaatcc gcccaactgg ttgttgacca gaactatacg
      421 gtggcagatg ccgccaaagc tatggatatc ggcctttcca caatgacaag atgggtcaaa
      481 caactgcgtg atgagcgtca gggcaaaaca ctaaaagcct ctccgatgac accagaacaa
      541 atcgaaatac gtgagctgag gaaaaagcta caacgcattg aaatggagaa tgaaatatta
      601 aaaaggctac tgcactcttg atgtcagcct ccctgaacag ttctctataa tagggaaact
      661 caggacacgt tatcctttgg tcactctctg tgtagtggat cggtaattca ggacacctca
      721 ttttcactgt taatgttaaa aagtgaaaat gaggtgatgc atgatcaaaa ctcgtcggac
      781 taaacgtacc ttttccccgg agttcaagct tgaagctatc gaacaggtgg ttaaatacca
      841 gcgtgatgtc agagaagtcg cgctggcact cgagctcaac cctgaccatt tgcgtaaata
      901 gatacggttg tataagcagg aacttcaggg tattgagccc gctggtaatg ctattactcc
      961 tgaacaacgc taaattaagc agcttaaagc gcagataaag cgccctgaga tggaaaaaga
     1021 aatactaaag caggctgccg tgctgatgag cgaaatcccc ggggaaactg tcgcgctaat
     1081 cacacagctg aaagcaaagt ggccagtgcg ggttctttgt catttattcg gtattcaccg
     1141 tagcatttat tacgcgcagg tgaagcgtcc tgttaatgtg caaagaattg aattacgaag
     1201 tcgggtgagg gctgtccatg ctctcagtcg tggcgcagtc ggtagccggg caatcagtca
     1261 gatgttgcgc catagtggcg ttgatgcagg ccggtggctg gcacgacgac tgatgcggga
     1321 atgcgggctg acaagtcgac agccagttaa acatcactac cgggtaaacg aagacaaaag
     1381 tccgccattg ccaaatttac tgaaccggca atttcacccc gccgcaccaa accgcgtctg
     1441 gtgcggcaac atcagtttta ttcgcctgca ggacaaatgg tgttatctcg cactcgttgt
     1501 tgatttatat tcacggcgga ttatcggttc agccctctca ttaaccgctg atgctgagtt
     1561 ggtgtgtagg gctttgcgta atgcacggga aacgcgaccg cgtgatggcc accagctgtt
     1621 tcattcagat ctgggtgttc agtataaaag caataaatac agaaagttac tctggcgtta
     1681 tggggtaatg caaagtatga gacgcagagg gaattgcctg gataattcgc cgatggaaag
     1741 agtctttcgc agccttaaaa gtgaatggtt tccgaaaggt ggtatggtga ttttagccat
     1801 gcggtacgcg atataaacca gtggattaat ggttatt
//
LOCUS       DBBGLQ010000309         1817 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.315, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000309 DBBGLQ010000000
VERSION     DBBGLQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.315"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>1817)
                     /locus_tag="ACIYSB_005595"
     CDS             complement(<1..>1817)
                     /locus_tag="ACIYSB_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGC5432377.1"
                     /translation="PAYSDNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCD
                     QSVPDGFGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDK
                     VWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGQETATELVEDTQAIAR
                     YGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFCVGAEGLRHVPGDVIEICD
                     DDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDG
                     VKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIV
                     DNGAYFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFMLRLT
                     VAADDGSERLVSTARTTETTYRFRQLAPGNYRLTVRAVNARGQQGDPASVSFRIAAPA
                     APSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETTARYLGTALYWI
                     AASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSNDPAAYLNFFRGAINKTHLGREI
                     NERIDASALRTEVEQLENEINREMTQLEKDVRQRAERDIA"
BASE COUNT          348 a          595 c          479 g          395 t
ORIGIN      
        1 gcgatatcac gctccgcacg ctgcctcaca tctttctcca gctgcgtcat ttcgcggtta
       61 atctcattct ccagttgctc aacttcagtg cgcagtgcag aggcatcaat gcgttcattg
      121 atctcccgtc ccagatgcgt tttatttatc gcccccctga aaaaattaag gtaagcggca
      181 ggatcattac tcggttgacc gactgcctcc acgaatgccg atttaccgac agtgttaaca
      241 ctgcggatat aaaagtaata atcatggccc ggtttgatat tgatactggc ggctatccag
      301 tacagcgccg tgccaagata gcgggctgtg gtttcaacct gcctgatatc cgcaatccgc
      361 ttttccgaga accagaactc aaactgtacc gtcggatcat aaaccgcaag atgcggcgtg
      421 gcggttatct gaaaatagcc cggcgtcagc tcaatccgcg atggcgctgc cggtgcggca
      481 atccggaacg ataccgacgc cggatcgccc tgctgccccc gggcatttac tgcccggact
      541 gtcagcctgt agttccccgg cgccagctgc ctgaagcggt atgtggtttc cgtcgtccgg
      601 gccgtgctga ccagccgctc actgccgtca tccgctgcca cggtcaggcg aagcataaag
      661 ctcacgccct tcaccacctt cggcgtatcc cagcgtgcca gcacctggta ttccccgctg
      721 tctgcggtga cttctgcggt caggtgttgc actgctggcg gcgtgacacc attcaccgtg
      781 ccgctctggt cgccgtcaaa gtacgccccg ttatccacga tggcctcttt ttccggtaca
      841 tgctgcacgg cggtgatggc atacgtgccg tcgtcgttct cacggatact cacgcagcgg
      901 aacaggcgct ggcgcagcgt cggcagcttc agcccccaca cgctgtattc agcaacaccg
      961 tcaggaacac ggctcacttt caccttcacg ccgtcggtga cggactgaac ctccacgctg
     1021 accggattgc cacttccgtc aaccaggctt atcagcgtgg taccggagga tggcagcgtg
     1081 atttcacggt cgagcgtcag cgtccgggtc tggctgttta ccgccagcac gcgcccgccg
     1141 gtgctgatac cggcatagtc atcatcacag atttcaatga catcgcccgg cacatggcga
     1201 agcccttctg cgcccacgca gaagtccacg gtctgcgtct ccagcagttc tgttttaatc
     1261 agccacagcc cggcgcggtg tgcctgcccc cggctggtac agccaaaagc atccatcttc
     1321 gtgacgttac gaccgtaacg ggcaatggcc tgcgtatcct ccacaagctc tgtcgccgtc
     1381 tcctggccgt tgttcgggtc aatccagttc acctcaacgg cattatggcg gtccttgagg
     1441 gcgctgaagc tgtagcggaa cggcgcgcca tcatccggca tcaccacatt actgcggtta
     1501 taggtccaca ccttatccga cggtcggtcc tgcacgaacg tcagcgtctg cccgttccat
     1561 actggcatac agcgcatcgc cgagcagaaa tcactgagca catcccacgc cttgcgctgt
     1621 gtggtcaggt acgcattaca ggtgatgcgc ggctccgtgc cgccaaagcc gtccggcact
     1681 gactggtcgc agtactggcc gatgacatac agcgcccatt tgtccacatc cgccgcacca
     1741 agacgtttcc ccatgccgta gcgcgggtgg gtcagcatat cccacagaca ccaggccggg
     1801 ttatcgctgt atgccgg
//
LOCUS       DBBGLQ010000310         1815 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.316, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000310 DBBGLQ010000000
VERSION     DBBGLQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.316"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..384
                     /gene="psiB"
                     /locus_tag="ACIYSB_005596"
     CDS             <1..384
                     /gene="psiB"
                     /locus_tag="ACIYSB_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGC5432378.1"
                     /translation="YEDIRAAGSDERRELTHAVMRELDAPDNWTMNGEYGSEFGGFFP
                     VQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVIQVQRRFAPEAVSHSLAL
                     AASLDTQRYSVNDIIHILMAEGGQA"
     gene            381..1167
                     /locus_tag="ACIYSB_005597"
                     /pseudo
     CDS             381..1167
                     /locus_tag="ACIYSB_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            1322..1471
                     /gene="mok"
                     /locus_tag="ACIYSB_005598"
                     /pseudo
     CDS             1322..1471
                     /gene="mok"
                     /locus_tag="ACIYSB_005598"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            1413..1538
                     /locus_tag="ACIYSB_005599"
     CDS             1413..1538
                     /locus_tag="ACIYSB_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HGC5432379.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
BASE COUNT          371 a          472 c          556 g          416 t
ORIGIN      
        1 tatgaagata tccgggctgc cggaagtgat gaacgccgtg agctgactca cgccgtgatg
       61 cgggagctgg atgcgccgga taactggacg atgaacggcg agtacggcag cgagtttggc
      121 ggctttttcc ccgtccaggt ccgtttcacg cctgcccacg aacgttttca cctggcgtta
      181 tgttcaccgg gcgatgtctc ccaggtctgg gtgctggttc tggtgaatgc cggtggtgag
      241 ccgtttgcgg tgattcaggt acagcggcgg tttgcaccgg aagccgtcag ccattcactg
      301 gcactggcgg cgtcactgga tacgcagagg tacagcgtta acgacatcat ccatattctg
      361 atggcggaag gaggtcaggc atgagcgcac gttcacaggc actggttccc ctcagcgcag
      421 agcaacaggc cacatggcgg gcggtggcgg agacggaaaa acgccgccat cagggaaaca
      481 cgctggcgga atatccgtat gcgggagcat ttttccgctg tctgaacgga agccgccgga
      541 tttcgctgtc tgacctgcga tttttcatgc cgtccctgac cgcagaagaa ctgcacggca
      601 accgtcttca gtggctgtgt gccatcgatg tgctgattga aacacagggc gaggtctgcc
      661 tccttcccct tcccggcgat gcagcagaac gcttgttccc gtcggtgcgg tttcgtgtca
      721 gggaacgcag tcgccataaa tccgcgctgg tcatgcagaa gtacagccgc cagcaagcga
      781 gggaggcaga gcagaaagcc cgtgcgtacc aggtgctggt tgctcaggct gaaatcgaac
      841 tggcatttca ttcaccggaa acagtcggaa gctggcatgc acgctggagt gaccgggtgg
      901 cagagcatga cctggaaccc ctgttctggc agtggggcga gcgttttccg tcactggccg
      961 gaatggaacg ctggcagtgg caggatatgc cattctggca ggttatcgcg gaagccagtc
     1021 tggcagccag agaggccggt catgctgtcc gtgagatgga gcggtggatg gtgccgaaca
     1081 aactccggga ggcagcgtga tgcggcaata atcacacgga ttacccgtga actggttgaa
     1141 cgaccagatt attttcaggg aaagtgagtg tggtcagcgt gcagggatat gagctatgat
     1201 gtgcccggcg cttgaggctt tctgcctcat gacgtgaagg tggtttgttg ccgtgttgtg
     1261 tggcagaaag aagatagccc cgtagtaagt taattttcat taaccaccac gaggcatccc
     1321 tatgtctagt ccacatcagg atagcctctt accgcgcttt gcgcaaggag aagaaggcca
     1381 tgaaactacc acgaagttcc cttgtctggt gtgtgttgat cgtgtgtctc acactgttga
     1441 tattcactta tctgacacga aaatcgctgt gcgagattcg ttacagagac ggacacaggg
     1501 aggtggcggc tttcatggct tacgaatccg gtaagtagca acctggaggc gggcgcaggc
     1561 ccgccttttc aggactgatg ctggtctgac tactgaagcg cctttataaa ggggctgctg
     1621 gttcgccggt agcccctttc tccttgctga tgttgtacgg gcatgaacaa cctgacttca
     1681 ggaaggtcgt ttcccttcag gaacggggat gaacgcgcgc ctgcggcgcc cgttcttttc
     1741 ccccgccttc tctggttatg gcctgtcaaa aatcacatca ccttccctgt gattattctc
     1801 tttttcttcc tgctc
//
LOCUS       DBBGLQ010000311         1807 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.317, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000311 DBBGLQ010000000
VERSION     DBBGLQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.317"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(324..724)
                     /locus_tag="ACIYSB_005600"
                     /pseudo
     CDS             complement(324..724)
                     /locus_tag="ACIYSB_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539895.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(740..1059)
                     /locus_tag="ACIYSB_005601"
                     /pseudo
     CDS             complement(740..1059)
                     /locus_tag="ACIYSB_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612263.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1105..1445
                     /locus_tag="ACIYSB_005602"
                     /pseudo
     CDS             1105..1445
                     /locus_tag="ACIYSB_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          533 a          392 c          357 g          525 t
ORIGIN      
        1 gacttgtatg taaagtcaag attaacgttg ctaaagcacg catcaatttt ctgataaaag
       61 gatggggttg tttttttcga taaaaaaaat tcttgtctaa ccactggtga aataagatta
      121 ttattacccc gaattaatac tgagcctaac aatagaatac aaaaaaaaca aaatgcttta
      181 aataagggga gcactataag ttatcctctt tactttatgc ttaccaactc atttatgtta
      241 ctttataaca taacccatct tgcgttgtaa acagactcgt tttagttcca tgcacctttt
      301 gagatcgtga ccaaataatg gcgttagtga tgttcttcca gtgtcatatg gttaagtagt
      361 tgatatggcg ttcactgtta tattctgaca accagtttgc cgccagttcc cgcttttcat
      421 ccagcgttct gaacagacaa aaaccgagta tttttgttcg gtatgtccgg ttaaaactct
      481 cgataaaagc gttctgcgcc aacttacctg attgggtaat ctccagtttt actacatgtt
      541 tttctgcgca ttacgtccgt gttgaataaa aaaaatcctg gccataatcc atgcgaatca
      601 tagctggata gacatggttt tccgcaatcc tacagagtac cgctaccact tgtgaagctg
      661 gcagattcag attgttttca atcgacaacg cctcatagtt aaagtcatca acgacattga
      721 acgtgtgaag tggtcgacaa aaactggcca ccgagttaga gtttttccag tatcgatttt
      781 ccgattcgtt tggggataac ccaccgttat attcgtgcgg tcttagtgcg ctgtaatatc
      841 caacgatata gaccgttatg gcgtgagcgg cctcgcagaa gcttcgtaac ccaccaccgg
      901 catccattcg ttcttcagac tcctgaagaa gcgttccatt gggctgttat cccagcagtt
      961 tccgcgctgg ctcatactct gcctgatctg gtatcgccac aataactgcc ggaactgcct
     1021 gctcgtatcg ctgtggaaca tcaccccgcc gggcttaccc atccagatat aggtcacatc
     1081 accgcaccac acctgtatag cggcgtaatc cccaaaaacg atgctggttg tcagtaaaca
     1141 agccatgaat gacagtcgtt cctgaaatca taagacctgg aggagtgtat gctccgtcgc
     1201 agtaattacc acgatacgct tgcagaattt tttccagtta ctgaagcgtg aacggataaa
     1261 gaaaaagctc ttcggaacgc gggaagacgc ctccaacgat atttttgaac atcgaaatgt
     1321 tttataacaa taagcgtcgg catggttcct gcgaacagat gtcaacaata gaatataaaa
     1381 acaattatta tcaacaactt ggaaggagta agactacccg tggcgattca atattagatc
     1441 attgatggag atataaatgg ctcacttttt atcggttgtt gacgatacgt ttatcaagta
     1501 tacatgaaaa tttcttcata ccagctatat gagacgttaa gactaaaaat cttactccca
     1561 aaatgactat gttgttgcag tttggtttat tgactcttgt ctatagttca ccgaatgcca
     1621 agatgaggga gcgaggaact ttactgtcta cgcctctctt cctggttaca aagacaagcg
     1681 taaaactcac catccattag aactaaaggc actttcctga gcagagtttt catctgcaag
     1741 ataaaagcta ttttacagcc gtaaagtcca gtgaaacgaa agagaggtta gtgaattaat
     1801 tacttga
//
LOCUS       DBBGLQ010000312         1797 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.318, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000312 DBBGLQ010000000
VERSION     DBBGLQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.318"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(1..215)
                     /locus_tag="ACIYSB_005603"
                     /pseudo
     CDS             complement(1..215)
                     /locus_tag="ACIYSB_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(418..1302)
                     /locus_tag="ACIYSB_005604"
     CDS             complement(418..1302)
                     /locus_tag="ACIYSB_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGC5432380.1"
                     /translation="MPFSNSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRHQELSLATVTARVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGDAIGKSFDDAVARFTFPVWMLQLLR
                     KARDRIIRLLETLWDRLF"
     gene            1403..>1797
                     /locus_tag="ACIYSB_005605"
     CDS             1403..>1797
                     /locus_tag="ACIYSB_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432381.1"
                     /translation="MAKNKGICRNKKCSNPVSEFDEANALRLCDDHYKLWKRNKENRR
                     VRNICRIISRYDKSLDWEIFYRVRDAAKNKTGKRIIEDAKILHQWRASKQVRKSFHKG
                     VLRSREWLNTQELLSRKHISVTGELFKFY"
BASE COUNT          525 a          367 c          460 g          445 t
ORIGIN      
        1 gggctgctct gtcgtctgag gctgtacctg ccatttctgt actctaaatc caggtgaacc
       61 agacgctaat gaaaatcgcg ctgtaaagta cgttctgtaa cgccaaattc atctgacagt
      121 gtctttagtg acagagattc tccggccatc agtcggctga taatgagtga taagcgaacc
      181 gccagacgat catgccggga acgatcatca gacatagatg tgtactccgt aagaaaacat
      241 cttttatttc atgatgttat tatgcctcat ggtgttgaaa ggatgtgtcg tatctgaata
      301 atcaggaata aaatgcagca gaaaaaatat tcctttaatt atctgatttt taatgatgat
      361 ttcataaaaa gaagaatact cgttaatcca gcgacacatc ctgtcggcgt gagtgtgtca
      421 gaacagacga tcccacagtg tttccagcag gcggataatg cggtcccggg ctttacgcag
      481 aagctgtaac atccagaccg gaaaagtgaa ccgggcaacg gcgtcgtcaa aacttttccc
      541 gatggcgtca ccaaaagtct gttgtgcgtg tttctgggcc tgttcagagc ggttttcccc
      601 ccggatatgt gaataaaccg cactggttgc ctgcggtggc agggcgtgga tcatcagcga
      661 cacaaacgcc ggaagattcc agccgacagg cgcagcaacg gggagtaccg gaaatgatga
      721 cgggaacagg gttgccaccc tggctgtaac agttgccagt gagagttcct ggtgacggga
      781 tgggcatttt tctgtggcat tccattcttc agcaggaaac acgcgatcgg catggctgag
      841 aacaaacaga aaacgcgagg gatctgcacc ttcattcagt aaaaactgat gcatggcaat
      901 atcggcagca tacgcacgtt catcagcccg caggatccag ataatcagat ccagttccgg
      961 cagtaactga cggtaaagct cccggtattc ctgatcatgc tgcggtgtct caccaatgcc
     1021 gggcagatcg accagcgtca ttctgcgttc accaatctgc agggtgaggc gatgagcctg
     1081 gcgggtgcag ccgttcaggg gatgcgtggc gcagatacgg gactgaaaaa tggcattaca
     1141 cagactgctt ttgccggtgc cggatttacc cataatgcca atcacgggct cgtaatgaat
     1201 aacaccgtgg agatgttcaa ggattttctg gcgaatttct tcaggaagaa acgtcagata
     1261 tgatttcact tgcgcggaga gggaagaatt gctgaatggc ataccggtat cctgttgatt
     1321 tcactgaacc ggatcacact aatccggttt atcattcagg taatatgggt gtgaaaattt
     1381 ttccattgac gggaagaaga gtatggctaa aaacaaaggt atctgtcgga ataaaaaatg
     1441 cagtaatcct gtttcagaat ttgatgaggc aaatgcctta aggttatgcg atgatcatta
     1501 taagctatgg aagcgaaata aagagaatcg tcgggtcagg aatatttgtc gtataattag
     1561 tagatatgac aaaagtctgg actgggagat attttataga gtaagagatg ctgcaaaaaa
     1621 taaaacggga aagagaatta ttgaggacgc aaaaatatta catcaatggc gtgcttcaaa
     1681 gcaagtaaga aaaagttttc acaagggtgt gcttcggtca cgggaatggc ttaatacaca
     1741 ggagttactg agcagaaaac atatatctgt tacaggtgaa ctatttaaat tttacat
//
LOCUS       DBBGLQ010000313         1792 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.319, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000313 DBBGLQ010000000
VERSION     DBBGLQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.319"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..477)
                     /locus_tag="ACIYSB_005606"
     CDS             complement(<1..477)
                     /locus_tag="ACIYSB_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGC5432382.1"
                     /translation="MNRLRFEVKNDQGCFIFPETWYESLLEKFDELLDAFDEDELSDR
                     SYINALKRITQQEPDFIDAYAYMAYAFLEQETPRKALNIALKGLAAGNRLIPEGFTDE
                     ISWLHPENRPFLNALYAAILANVHLQHHQNAVMLIDKILTYNPSDNPGARWLLGPEL"
     gene            complement(843..>1792)
                     /locus_tag="ACIYSB_005607"
     CDS             complement(843..>1792)
                     /locus_tag="ACIYSB_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5432383.1"
                     /translation="YEKQARKLAIRESMACTFRTIAEELLTHMARRWTTGYQADQRAR
                     LENHVFPALGNRPVTGIRPMEILNVLRSIEEQGKLEALRKTRQLCAQVFDYAIATGRA
                     TVNPASTLSGVLLPPEKQQRRTLSEEQIGAFMHQITASGNLMSLATRMLVLTAVRTGE
                     LRLATWDEINFTDALWVIPKERMKMRRPHVVPLSRQVLALLEEIKTRTESRNSAFIIP
                     GAFRKDLPRNRKCINEFLEQSGWHALTTAHGFRHTFSTILHDHDFSSAWIELQLAHVD
                     KNVMRGTYNHAQYLEGRREMMQWYADYIDSLMEKKVQTK"
BASE COUNT          441 a          414 c          431 g          506 t
ORIGIN      
        1 tagttccggt cccagtaacc agcgtgctcc cggattatct gaaggattgt aagtcagaat
       61 tttatcaatg agcatgacag cattctgatg atgctgcagg tgcacattcg cgaggatagc
      121 ggcgtaaagg gcattcagaa agggcctgtt ttccggatgt aaccagctga tttcatcggt
      181 gaaaccttcg ggtatcagtc ggttacctgc cgccagtcct ttgagtgcaa tattcagcgc
      241 ttttcgcggt gtctcctgct ccagaaaggc atatgccatg tatgcatacg catcaataaa
      301 atccggttcc tgttgtgtga tacgcttcag ggcattgata taagacctgt cgctgagctc
      361 atcctcgtca aaggcgtcaa gcagttcgtc aaacttctcc agtagtgatt cataccaggt
      421 ttccgggaaa atgaagcagc cctgatcatt cttcacttca aagcgtaatc tgttcatatg
      481 ctgaatctgt tgtaaatctg gaaaaactta tggttcttca agaataaaac ccacggcagt
      541 ttgtatcaag tttcttctca taacggtaac agcaggccag agatattttt gtgttagtca
      601 tggattcatt tttcaatgag gagcaatcag cctgactggt aatgatgctg tagaaatgtg
      661 gctgcgaggt cagaacagca gtgtgctgct atatgaaaat gacgatgtga agctatcatc
      721 attttggtaa ggcgtcttag tgcgctcatc agatgatatt gaatatatct cctgaagtca
      781 gaaatcaaaa aatacaaatg gtagttacct ccccaccaac cacagcggtt agtggggagg
      841 acttatttcg tttgtacttt tttctccata agtgaatcaa tgtaatcagc gtaccactgc
      901 atcatttcac gacgtccctc cagatattgc gcatggttat aagtgccacg catgacgttt
      961 ttatcaacat gggcaagctg gagttcaatc caggctgagc tgaaatcatg gtcatggaga
     1021 atggtgctga aggtatgacg aaatccgtgg gctgttgtca gggcatgcca gcctgactgc
     1081 tcaaggaatt cattgataca cttccggttt cgcgggagat cttttctgaa tgccccggga
     1141 ataataaagg cggaattacg cgattcagtg cgtgttttta tctcctccag taacgccagt
     1201 acctggcgtg aaagcggcac aacgtgcggt cgtcgcattt tcattctttc tttcggaata
     1261 acccacaggg catccgtgaa attaatttcg tcccaggttg ccagtcgcag ttctccggtg
     1321 cgaacagcag tcaaaaccag catccgcgtt gccagcgaca tcagatttcc ggaagccgtt
     1381 atctgatgca tgaaagctcc tatctgttct tcagaaagcg tcctgcgctg ttgtttttcc
     1441 ggaggaagca gtaccccgct gagtgtcgat gccggattaa cggttgcacg tcctgttgct
     1501 atggcataat caaaaacctg cgcacagagc tggcgtgttt tacgcagtgc ttcaagtttt
     1561 ccctgttctt ctatactcct caggacgttc agtatttcca tggggcggat cccggtcaca
     1621 ggacggtttc caagagcagg gaaaacatga ttttcaagtc ttgcacgctg gtctgcctgg
     1681 taacctgttg tccatcgacg tgccatatga gtaagcagct cctctgcaat ggtgcgaaat
     1741 gtgcaggcca tactttccct gatggcaagt tttcgcgcct gtttttcata cg
//
LOCUS       DBBGLQ010000314         1791 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.320, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000314 DBBGLQ010000000
VERSION     DBBGLQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.320"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..270
                     /locus_tag="ACIYSB_005608"
     CDS             <1..270
                     /locus_tag="ACIYSB_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGC5432384.1"
                     /translation="HITAKDNAWLYSLSHQTNGTGESEWIHFTGSGYLLRTDAWSYPV
                     LRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTFDW"
     gene            282..479
                     /locus_tag="ACIYSB_005609"
     CDS             282..479
                     /locus_tag="ACIYSB_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGC5432385.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDRTDSAALLPC
                     IEQAREDIRILRQLQLLQQNR"
     gene            564..1430
                     /locus_tag="ACIYSB_005610"
     CDS             564..1430
                     /locus_tag="ACIYSB_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGC5432386.1"
                     /translation="MSEITAPRPEVVNGHTDVIGSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVLNVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            1502..1765
                     /locus_tag="ACIYSB_005611"
     CDS             1502..1765
                     /locus_tag="ACIYSB_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGC5432387.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVAAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
BASE COUNT          521 a          434 c          444 g          392 t
ORIGIN      
        1 catatcacgg caaaggacaa cgcctggctg tactcgctgt cacatcagac gaatggcacc
       61 ggcgaatcag aatggattca tttcacaggt agcggatatc tgttacgtac cgatgcgtgg
      121 tcatacccgg ttctgcggct taagcgcctg ggcctgtcaa aaacgttccg tcgtctggtt
      181 gtcacactta cccgacgtta tggcgtcagt ctcattcatc tggatgccag cgctgaatgt
      241 ctgccggacc tgcccacttt cgactggtaa ccaggaacaa catgaaatca ttaaccacgg
      301 aaaccgcact ggatattctg attgcgtggc tacaggacaa tatcgactgc gaatcgggaa
      361 ttatctttga caacgatgag gatagaacgg attcggcagc actgttgccc tgtatcgaac
      421 aggccaggga ggatatccgt atcctgcgcc agttgcagct tctgcaacag aaccggtgag
      481 tcttactcac cagacttcat tccactgacg ccagcctgaa cagggctggc gttttcattt
      541 atctgcaaaa aggaatatcg attatgtctg aaatcacagc cccccgtccg gaagtggtca
      601 acgggcatac ggacgttatc ggctcaacca gcatccgcca cattctggct gtacgaaaga
      661 gtgcgctgct gcaaatcgac acacttatcc ggcaactggc agaaatctcg gtgctgacgg
      721 aaagcatcgg aggtaaaact gcactggact gggccatgaa acaggatttt cgctgcggtt
      781 gctggctgat ggaaaaaccg gaaaccgcaa tgaaagccat cacccgcaac cttgaccgcg
      841 aaatctggcg tgatctgatg caacgttcag ggatgctgtc attaatggat gcacaagccc
      901 gtgatacatg gtaccggtca ctggagtacg ataattttcc ggaaatcagc gaagcaaata
      961 tcctgagcac gtttgaacaa ctgcaccaga acaaagatga agtgtttgag cgaggggtgc
     1021 tcaacgtttt cagaggactg agctggaatt acaaaaccaa ttcaccctgc aaatttggca
     1081 gtaaaattat cgtcaacaat ctggtgaggt gggaccggtg ggggtttcat cttatcaccg
     1141 gacaacaggc cgatcgactt gccgatctgg aaagaatgct gcacctgttc agcggcaaac
     1201 cgatccccga caaccgggaa aacatcacca ttcatctgga tgatcacatc cagtctgttc
     1261 agggtaaaga ggactatgaa gatgagatgt tcagcatcag atactttaag aagggctccg
     1321 cgcacatcac gttcaggaaa ccagagctgg ttgacaggct taatgatatt attgccagac
     1381 actacccgga agccctggca agcacagtga acaaaagccg caaggcataa aagaggtatt
     1441 ttacaattcg cgaatattaa ctattattaa ttgatagtaa tcaatcggcg agaaaataac
     1501 catgcctacc agtgtatccc tcagtcctta ctttgaaacg tttatccgcg aacaaataga
     1561 aagtggtcgg tacaacaata caagtgaagt cattcgtgcc ggacttcgtg ctctggaaga
     1621 acgggaacag cagataaagc ttgaatcact ccagagcgcc gtggccgcag gcatcaacag
     1681 cggtgaaagt aaaagtgctg aagaggtatt cgggcgactg acgcacaaat acaaaaagat
     1741 ggcagaaggt gaacagccaa tatgaaactg actgtttcac cattagcaga a
//
LOCUS       DBBGLQ010000315         1789 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.321, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000315 DBBGLQ010000000
VERSION     DBBGLQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.321"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..480)
                     /locus_tag="ACIYSB_005612"
     CDS             complement(<1..480)
                     /locus_tag="ACIYSB_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGC5432388.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     "
     gene            complement(477..971)
                     /locus_tag="ACIYSB_005613"
     CDS             complement(477..971)
                     /locus_tag="ACIYSB_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGC5432389.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(1097..1447)
                     /locus_tag="ACIYSB_005614"
     CDS             complement(1097..1447)
                     /locus_tag="ACIYSB_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGC5432390.1"
                     /translation="MPPRTPKACRVRGCPHTTTDSSGYCESHKSEGWKQYKSGQSRHQ
                     RGYGSKWDVIRERVLKRDKGLCQLCLRAGVVREAKTVDHINPKSHGGTDVDSNLQSLC
                     WPCHKAKTARERLK"
     gene            1598..>1789
                     /locus_tag="ACIYSB_005615"
     CDS             1598..>1789
                     /locus_tag="ACIYSB_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432391.1"
                     /translation="MNSYEWEWLLNKDDTLCFFPVGHSVEGNYKIHFELSGSCNLRVS
                     DAEWHGKPVLLFEYFDEDDD"
BASE COUNT          381 a          491 c          467 g          450 t
ORIGIN      
        1 tgccggacga aagacttccc atgcctgttt ctccgtcgtg gcaccggaat acacttccgc
       61 gccgaactcg ttatcacagg caaaacaata cagggcgaca ccggcagaga ttgctgattt
      121 gccgttctta cgggggattt cggtatacac ctcccggaag cggcgcagcc gggtaccttt
      181 attgacccag ccaaacgcac agcagatcac aaagagctgc cacggctcca gcgtgatggg
      241 catccgttta aatgcccact cacccttggt atgcggcaac agctgaataa atttggcggc
      301 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
      361 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
      421 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
      481 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
      541 cgctggcggc tgctggggtc gagtccgagc attgcccccg tactgctcat ctcggactcc
      601 tgttcttttt tggcggtcag ctccggattt ttgaccatgc cgcccattgc accggtgatg
      661 gtgttgccct gtctggcaat atttttcaca gcacgtcgcc agaactcata ggctacgcac
      721 caccgctcaa gcactgcgag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
      781 gttgtcagtt gccacatgat cgtggcgaga gggagctctt cttcagcgaa ccactccggt
      841 ggctcaacac ctttgatggg cgtaaaaacg ggttcatctt tgttcagggc tcgcttgccg
      901 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
      961 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     1021 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccccccc tcccctctgg
     1081 cagtgggaac tggttcttac ttcagccgtt cacgggccgt cttcgcctta tgacacggcc
     1141 agcacaaact ctgcagatta ctgtcgacat cagtgccgcc atgcgattta gggttgatgt
     1201 ggtcaacggt cttcgcctca cgcaccacac cagcacgcag acataactga cacaggcctt
     1261 tgtcacgctt gagcacacgt tcacggataa catcccattt cgaaccataa ccgcgctggt
     1321 gtcgggattg tcctgacttg tattgcttcc agccttcgct tttgtggctt tcgcagtagc
     1381 ctgacgagtc agtggtggta tgggggcagc cgcgaacgcg gcaggctttt ggggttcgtg
     1441 gtggcattgc ataatcctct tggatggttc gcgtgcgata cgtggcatcc ttctcagaat
     1501 cgaatcgcac tccactccgg ttttgccata aacgatcttt tatgcttcgc tggatgtagt
     1561 tgatagttgt tgaaactcaa tgaacaggag ttcacaaatg aatagttatg aatgggaatg
     1621 gcttttaaac aaagatgaca ccctatgctt tttccccgta ggccattctg tagaaggtaa
     1681 ttacaaaatt cactttgagt tgagcggtag ttgtaacctt agagtctcag atgctgaatg
     1741 gcatggtaaa cctgtactgc tgttcgaata cttcgatgaa gatgatgac
//
LOCUS       DBBGLQ010000316         1777 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.322, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000316 DBBGLQ010000000
VERSION     DBBGLQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.322"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            196..867
                     /gene="queE"
                     /locus_tag="ACIYSB_005616"
     CDS             196..867
                     /gene="queE"
                     /locus_tag="ACIYSB_005616"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGC5432392.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1207..1600
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1207..1234
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaacc"
BASE COUNT          477 a          383 c          415 g          502 t
ORIGIN      
        1 aattttggtg aatgttttga catgattaaa tccattttct ttattttctt ctgacataat
       61 acgttactcc ctttaattat taacgatatg tttcgcattt ttctttaaac ctgcctgtaa
      121 cagaatgtta ataagacatg attgttgtgg caggatctgc aataattaac aattatccac
      181 ttcacagaga atgctatgca gtacccgatt aacgagatgt tccagaccct gcaaggtgag
      241 ggttacttta ccggcgttcc cgccattttt attcgtttac agggatgccc ggttggctgt
      301 gcctggtgcg acaccaaaca cacctgggaa aagcttgagg atcgggaagt ctcccttttc
      361 agcattctgg cgaagaccaa agagagtgat aagtgggggg ctgcgagcag tgaagatttg
      421 ctggctgtca ttggtcgcca gggatacacc gcgcggcatg tggtgattac gggtggtgag
      481 ccttgcattc atgatttgct gccactgact gatctgctcg aaaagaacgg ttttagctgc
      541 cagatcgaaa ccagtggtac tcatgaggta cgctgcacac cgaatacctg ggttaccgta
      601 tcgccaaagc tgaacatgcg cggcggctat gaagtgttgt cacaggcact ggagcgagcc
      661 aacgaaatca agcatccagt ggggcgcgta cgcgatattg aagcactgga tgaactactg
      721 gcaacgctga ccgatgataa accgcgagtc attgcactgc agccgattag ccaaaaggat
      781 gatgccacac gtttgtgcat tgaaacctgc attgcgcgta attggcgttt gtcgatgcaa
      841 acacataaat atctaaatat tgcctgatta aacatttata agcgttataa atgggtggaa
      901 cctgtaaaga cttctactca tttatattgt ttgtcgcctc tgaaaactcc tccattttac
      961 ccatccaggg ctaatcatta gcattctcta caaattctgt ggcattaatt tttcgctgga
     1021 gtgaaaatta ttgcggtaaa gtttggtaga ttttagtttg tatagagtta ttttaaatat
     1081 ttaccttttt aatcaatgga ttaagtgctc tttaacataa tggatgtgtt gtttgtgtga
     1141 tactataaag ttggtagatt gtgactggct taaaaaatca ttaattaata ataggttatg
     1201 tttagagtgt tccccgcgcc agcggggata aaccggcaaa aaccgggcaa tcgcaaaaag
     1261 gcgtaatgtg ttccccgcgc cagcggggat aaacctgtgt ttgcggcatt aacgctcacc
     1321 agcatttcgt gttccccgcg ccagcgggga taaaccgacg tggtcatggg tgctgctgtt
     1381 gcagagccag tgttccccgc gccagcgggg ataaaccgag cagatacacg gctttgtatt
     1441 ccgtgcgccc gtgttccccg cgccagcggg gataaaccga atagcaatag tccatagatt
     1501 tgcgaaaaca ggtgttcccc gcgccagcgg ggataaaccg gagcctgacg agactactga
     1561 ggccgttctg tcgagttccc cgcgccagcg gggataaacc accatataac ccgttatctc
     1621 tttctcaagt ttttatatta gcagtacttg taataagcaa catatccacg taacacctca
     1681 tgttcaaaat agttctccat gccagagagg ttcacaatta tcgatacaaa aaattaaatt
     1741 taatcaaagt gttatttgta tgattcttaa atcgtta
//
LOCUS       DBBGLQ010000317         1775 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.323, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000317 DBBGLQ010000000
VERSION     DBBGLQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.323"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..1239)
                     /locus_tag="ACIYSB_005617"
     CDS             complement(<1..1239)
                     /locus_tag="ACIYSB_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGC5432393.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSEKATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTAAEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLLRPLLPFYTALLVAL"
     gene            complement(1239..1748)
                     /locus_tag="ACIYSB_005618"
     CDS             complement(1239..1748)
                     /locus_tag="ACIYSB_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGC5432394.1"
                     /translation="MDAKREYTNCAFPRVIAAFSVAVMLALVVCVVWQVFSRYVLNQP
                     STLTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINILILGF
                     AGSVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQ
                     LKQPAQEAS"
BASE COUNT          577 a          426 c          394 g          378 t
ORIGIN      
        1 caatgcgact agcaatgctg tataaaaggg tagcaatggt ctaagcaaat gttgaatctt
       61 cacacctgaa atagaacaac caacaaatag cgcactaccc accggcggtg ttaacagccc
      121 aatacaaagg ttggccacca tcataatacc gaaatgtacc ggatccattc ccagctcttg
      181 tgcaatcggc aagaagattg gtgtgaagat tagtacagcc ggtgtcatgt ccataaagat
      241 gccgacaatt aataagacga tattaatcag cagcaatatg accagtgggt tatcagaaat
      301 ccccatcaac gcgtcgctga tcatataagg aatatcagca ttggtcattg cccaggacat
      361 gcccacggaa aaaccgatca acagtaatac aatcgaagtg gtaacaaccg attccagaat
      421 cagtttgggt aaatcgcgcc attttacttc ccggtaaatc agcaccgaga gaataaatgt
      481 gtagaccacg gcaattgctg acgcttcagc tgcggtaaaa atgccgccca gaatgccgcc
      541 cattacaatg aacaccagaa gcaaacttgg taaggcatcc agaaacgctt tacatgcctg
      601 agcaaaggtt gctttttctg aaagtgggta gccacggcgt ttagcaataa tgccgcagac
      661 tatcattacc gcgagaccca ttaagatccc tggcaaatat cccgccataa atagcgatgc
      721 tacagaaacg ccgccagctg tcagggaaaa tacaattaac acgtttgagg gcggtatcag
      781 taatccagta atacaggatg agacgtttac agcggtagaa aatgccggat cataaccttc
      841 tttggtctga atgggattca gtgtgccacc aacggcagca gctgccgcaa cggctgaacc
      901 agaaatggaa ccaaacatca tattcgccag gacgttcaca tgaccaagcg aaccaggaac
      961 gcggccaacc attacctggg cgaggttaat caaacgtatc gcaataccgc cgctgttcat
     1021 cagcgttccg gcaaagatga aaaaggggat tgccaacagg gcaaagttat ccagtccatt
     1081 agccagtcgc tgcgaaacag tgattgccgc gatatcccag ggaaacatca acagcattga
     1141 agctacagtg gcaataccga ttgagaacga aatgggcaca ccaataaaga ccagcacaaa
     1201 aaaacagcca aacagtgtaa gcgcgatata actatccatt aactggcctc ctgcgcaggt
     1261 tgtttcagtt gttgaatgct ttgattaata aaacacaagg cataaaacat catcaccaat
     1321 ccacttaatg gcagaatgat atatacgtat cccatcggta tttgcatcgc ggcagatacc
     1381 tgtgatgttg ccagtgtttt atcaattaat ttcaggccac cagtcacaat gaccgaaccg
     1441 gcaaaaccta aaatgagaat gttaatgact atactaagaa gtaattgctt gcgcttattc
     1501 agggcgagag caaataaatc gatagagaga tgtctctgcg cgccgacggt atatgcagca
     1561 cctaataatc ctacccagat catcaaaaag cgtgccagct catcggttaa tgtacttggc
     1621 tggttaagta cgtagcggct aaatacttgc cagactacgc atacaaccag agcaagcatg
     1681 acggcgacag aaaacgcggc aatgacgcgg ggaaaagcgc aattcgtata ctcacgcttt
     1741 gcatccaatg gttttgtcca gaacgcgggc agcct
//
LOCUS       DBBGLQ010000318         1750 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.324, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000318 DBBGLQ010000000
VERSION     DBBGLQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.324"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..323
                     /locus_tag="ACIYSB_005619"
     CDS             <1..323
                     /locus_tag="ACIYSB_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGC5432395.1"
                     /translation="GLVATAINHNKNKSGKIISTDYGIMQINSVHIPELKKLGVIKNK
                     DELLKKPCLNIHTGAWILARHFQRCGVNWECLGSYNAGFSKNNTARRMIYARKIHNRY
                     FRQY"
     gene            283..990
                     /locus_tag="ACIYSB_005620"
     CDS             283..990
                     /locus_tag="ACIYSB_005620"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HGC5432396.1"
                     /translation="MPEKSTTGISDNINKYEVTVSALLSMTIIIIILAVILYLAYITA
                     ALYLSAVHDTRPPRPVVYVCCLLAIVSVSLNGAYGVEATGLLFLSILTGLLTVMTLTD
                     IAVCRLPRIFTLSLIVLGAAFRYSLEELTYSLLNASLWFGMTYLLRQFFITAKGTEAL
                     GLGDVFLIAGIAMWTQPQHTPLLITAAASGAFLFILLFCRHRHQQALPFAPFLCASLY
                     ALTLLPDSVFRTSEIFT"
     gene            987..>1750
                     /gene="pilV"
                     /locus_tag="ACIYSB_005621"
     CDS             987..>1750
                     /gene="pilV"
                     /locus_tag="ACIYSB_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HGC5432397.1"
                     /translation="MKKFRHDDDKGFILLEILAGLIVIGIATPLIYSEIENWLNEQLY
                     QSAASHADAYNTAARNYIADNNATLHNGSLPANFTADDLIRKGYLKQGFNHSPFGQSY
                     ITGIRRNQTTGRLEALTCSTGGQNIKEDGLRSVAGQLPGLGGYIGKNGTATGAFGAWT
                     DKPGDYGLTCSAGHIAVVMTGDDLQESDRLYRFQVPGRSELNQMHTAINMGGNDINNA
                     GNLNGQKATVKGDITSEDGWLITRNNKGWMNTTHGGG"
BASE COUNT          467 a          454 c          431 g          398 t
ORIGIN      
        1 gcggcctggt cgccactgcg ataaatcaca ataaaaacaa atccggaaaa atcatcagta
       61 cggattacgg aataatgcag attaattcag tgcatattcc tgaactgaaa aaactgggcg
      121 tcattaaaaa caaggatgaa ctgcttaaaa agccctgcct gaatattcat accggtgcct
      181 ggatactggc ccgccatttt caacgctgcg gcgttaactg ggaatgcctg ggcagctata
      241 acgccggttt cagcaaaaat aacacagccc ggcgcatgat atatgcccga aaaatccaca
      301 accggtattt cagacaatat taataaatac gaggtgacgg tgagcgcatt actgagcatg
      361 acaataataa tcatcatcct ggcggttatt ctttatcttg catacatcac cgccgctctc
      421 tacctgtcag cagtacacga cacccggccg cccaggccgg tggtgtacgt gtgttgcctt
      481 ctggccatag tgagtgtcag cctcaacggc gcatatggtg ttgaagccac ggggctgtta
      541 ttcctttcca ttctgacggg attattaact gtaatgacgc tgacggacat cgccgtctgt
      601 cggcttcccc ggatattcac gctttcactg attgttctgg gggcagcgtt caggtacagc
      661 ctggaggagc tcacatacag cctcctcaac gcatcactct ggttcggcat gacatacctg
      721 ctacggcagt ttttcataac ggcaaaagga acagaagcac tcggccttgg tgatgtattc
      781 ctgatagcag gaattgcaat gtggacacag ccacaacaca cgccgctgct aatcaccgcc
      841 gcagcttccg gggcatttct gtttatcctc ctcttctgcc gtcacagaca tcagcaggca
      901 ctgccgttcg ccccgtttct ctgcgcatca ctgtatgcac taaccctcct ccctgattcc
      961 gttttccgca cttcggagat attcacgtga aaaagttcag acacgatgac gacaaaggtt
     1021 ttattctgct tgaaatactg gcgggactga tagtgattgg tatcgccacg ccgctgattt
     1081 attccgaaat agaaaactgg ctgaatgaac agctttacca gagtgctgct tctcatgcag
     1141 atgcctacaa taccgcagcc agaaattaca ttgccgataa caatgccact ctgcacaacg
     1201 gcagccttcc ggcaaacttt acagccgatg atttgattcg caagggatac ctgaaacagg
     1261 gatttaatca ttctccgttt gggcagagtt atatcacggg tatccgccgt aaccagacta
     1321 caggacgtct tgaagccctg acctgctcaa caggggggca gaatatcaaa gaagatggtc
     1381 tgcgcagtgt ggcaggacag cttcccgggc tgggtggtta tattggtaaa aacggaacag
     1441 caaccggcgc attcggcgcc tggacagaca aaccggggga ttacggactc acctgttcgg
     1501 caggtcacat tgccgtcgtc atgacgggtg atgatctgca ggaaagcgac aggttgtatc
     1561 gtttccaggt cccggggaga tcagagctga accagatgca cacagctatc aacatgggcg
     1621 gaaatgacat caataacgcc ggtaacctca atggtcagaa agccacggtg aaaggcgata
     1681 taaccagcga agacggctgg cttatcacca gaaacaacaa ggggtggatg aacacgacgc
     1741 acggaggcgg
//
LOCUS       DBBGLQ010000319         1739 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.325, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000319 DBBGLQ010000000
VERSION     DBBGLQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.325"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..820
                     /locus_tag="ACIYSB_005622"
     CDS             <1..820
                     /locus_tag="ACIYSB_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGC5432398.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYLPQ
                     TNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            817..1386
                     /gene="ybfC"
                     /locus_tag="ACIYSB_005623"
     CDS             817..1386
                     /gene="ybfC"
                     /locus_tag="ACIYSB_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HGC5432399.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
     gene            1612..>1739
                     /locus_tag="ACIYSB_005624"
     CDS             1612..>1739
                     /locus_tag="ACIYSB_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGC5432400.1"
                     /translation="MELKKLMEHISITPDYRQAWKVVHKLSDILLLTICAVISGAEG"
BASE COUNT          566 a          337 c          382 g          454 t
ORIGIN      
        1 gcagatgcaa aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg
      121 cgcagaatac gatgaatggg gcaacctgct gaatgaagag aacccgcatc agctgcagca
      181 gcttatccgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg
      301 gggatggaat ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact
      361 ggcatcaaaa tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc
      421 tacgcccgat ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgactacaca ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga
      601 tgattcccag tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggccaaca actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga
      721 cattaataaa cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc
      781 taagatgaaa cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg
      841 ctgatgatat ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat
      901 gattgtttta ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat
      961 aaagccatgt ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa
     1021 ctgggtacat gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc
     1081 gggtcactac gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat
     1141 acagcagaaa aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc
     1201 tatcaaaaaa agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg
     1261 gtaaaatatt ttaaagatga gaaaccaact tatgctcgaa tgacagcaca tctcccgaac
     1321 aaaaatgagt cttacgaatg tttaataaag atacagcatg aactaattcg tagtgaagag
     1381 aaataaaatc tcttcctgag aaaacaaatt aatcattgtg aaaaattata taactcatgg
     1441 aataggtgtt tattttgcgt tccatttgca gggaaagatc acgtaacgct acttttttgt
     1501 actaaataat tcgaatttta agttcaacaa ttgagatact acttattgtc taaagctgtt
     1561 tttcatagct tatacatgat caaatactcc ttacataaat aaggtgaaca aatggaactt
     1621 aaaaaattga tggaacatat ttctattaca cccgattaca gacaagcctg gaaagtggtg
     1681 cataaattgt cagatattct actgttgact atttgtgccg ttatttctgg tgcagaagg
//
LOCUS       DBBGLQ010000320         1719 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.326, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000320 DBBGLQ010000000
VERSION     DBBGLQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.326"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            15..137
                     /locus_tag="ACIYSB_005625"
     CDS             15..137
                     /locus_tag="ACIYSB_005625"
                     /inference="COORDINATES: protein motif:HMM:NF015301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HGC5432401.1"
                     /translation="MCERHNINNGYFTTCLKKLNYINKVISELSRFYTDEIDYE"
     gene            722..970
                     /locus_tag="ACIYSB_005626"
     CDS             722..970
                     /locus_tag="ACIYSB_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019455433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGC5432402.1"
                     /translation="MFGKVEDKAKEVAGEVQEKYGELTDNCGHQVKGAARKIAAQGGQ
                     AAQEAADVVRNNVENNPFAAISIAAGVGLLIGLLIGRK"
     gene            1042..1395
                     /locus_tag="ACIYSB_005627"
     CDS             1042..1395
                     /locus_tag="ACIYSB_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432403.1"
                     /translation="MNAFKTLFLSALLVSTGVLADSSTHTGSLKNQASQQAEKISNRA
                     EEAAHKAEHRIEEKQQQAENRLRNGGEVSTTDKVKEDAQKAWNRTKETTEKAWNRTKE
                     AAQQAGDAVSEKVSE"
     gene            1479..>1719
                     /locus_tag="ACIYSB_005628"
     CDS             1479..>1719
                     /locus_tag="ACIYSB_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGC5432404.1"
                     /translation="MRTGNIQPRGPSRSLVDNIHRVFNTLTGIFHTRIELIAIELEEE
                     KKNILGLLLMVGLTLIFTVFGVVSLLIFILLSVSPE"
BASE COUNT          525 a          311 c          419 g          464 t
ORIGIN      
        1 cttccagaaa agttatttgt gaaagacaca atataaataa cggttacttc actacctgtc
       61 ttaagaaatt aaattatatc aataaggtta tttctgagtt atcgcggttt tatactgatg
      121 aaatagacta tgagtaatct tcagaggcat gttaatctca gggatattgt tttctgcagg
      181 aggcggatat acaggcctgt gcattttacc gattatttaa gtacacaatt tactgttcct
      241 gtgtcggcat cagtggttag ttcactacat tcgaacagaa ctgcctgcta agagttggag
      301 ttgatggtta tctattaaca gatatcttta gatggctgtc tgtataactt ctggagtttt
      361 tattcccaga gattcaggac tatttttcag tttgagagta tttattgatg gcgattgctt
      421 gttaaacctg tgagataacc atttcatgtt cctccattga gaccataagg acagtgtact
      481 acgtgagttc tgtgattgtg cagggaactg ttggaagtga gcatttaatg aaaatgccac
      541 ggctttgctg atatagactg gtgagtatca ggtgaataaa aacaacctac aatcatattg
      601 ttgaatttaa tatttccctc tttacgtgag gaggatacat ggctcgtgcg ttgctaaata
      661 ccagtggacg atataaatgt atcggattaa atcttagttc ataatgcaga aggagtatat
      721 tatgtttggg aaagttgaag ataaagccaa agaagtagcc ggagaagtgc aggagaaata
      781 tggtgaactg accgataatt gcggacatca ggtaaaaggt gctgcccgta aaatcgctgc
      841 acagggtggt caggctgccc aggaggcggc ggatgttgta cgtaataatg tagaaaataa
      901 tcccttcgcg gcaatttcca tcgccgcggg tgttggcctg ctgattggtc tgctcatcgg
      961 acgaaaataa tatcagatgc gggcgaacag cccgcatttt tgtgccagat aatattgttt
     1021 catctgtata aggagtaaag tatgaatgca ttcaaaacat tgtttctttc tgccctgctg
     1081 gtatcaacag gcgtactggc tgattcgtcc actcataccg ggagcttaaa aaaccaggcc
     1141 agtcagcagg ccgaaaaaat atctaaccgg gcagaagaag cagcgcacaa ggctgaacat
     1201 cgtattgagg aaaaacaaca gcaggcggag aaccgtctca ggaatggtgg agaggtgagt
     1261 acaactgata aggtaaagga agatgcacag aaagcctgga acagaaccaa agaaacaacc
     1321 gaaaaagcct ggaacaggac taaagaagcg gcacagcagg ccggagatgc tgtaagtgaa
     1381 aaggtgagcg aataattctc cttccccggt cagaccgggg aaaatggagc atgcagcgtg
     1441 ctcagcagaa caatgattaa acaaaaaagg atgatgtctt gagaacagga aatatccagc
     1501 cacgtggacc atcccgtagt ctggtagaca atatacaccg tgtcttcaac acccttacag
     1561 gaatatttca tacccgcatt gagcttattg caattgagct ggaagaagaa aagaaaaata
     1621 ttctggggtt gctgctgatg gtggggttaa ctctgatatt cactgtattt ggggttgtat
     1681 cgcttctgat attcattttg ctgtcagtct caccggagc
//
LOCUS       DBBGLQ010000321         1716 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.327, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000321 DBBGLQ010000000
VERSION     DBBGLQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.327"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(333..1286)
                     /locus_tag="ACIYSB_005629"
     CDS             complement(333..1286)
                     /locus_tag="ACIYSB_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HGC5432405.1"
                     /translation="MYLKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGK
                     AKERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMV
                     DRDWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYS
                     FTAKGGSYIYSSEGGFRDETGSFPDGERAIGYKQHFKMPYIGLTGNYLYDSFEFGGSF
                     KYSGWVKASDNDEHYNPEKRITYRSDVNNQNYYSVSLHAGYYITPAAKVYVEGTWNRI
                     TNKKGDTSLYSRNLNISDHTKNGAGIESYNFMTTAGLKYYF"
     gene            1508..>1716
                     /locus_tag="ACIYSB_005630"
     CDS             1508..>1716
                     /locus_tag="ACIYSB_005630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc transferase"
                     /protein_id="HGC5432406.1"
                     /translation="MLVTANKALVGHFMPGTSVTWKPVPSSWVYFVNSYLKGDKMAKS
                     CDYEAIRGFISVNVGKDTNNAITVN"
BASE COUNT          484 a          363 c          327 g          542 t
ORIGIN      
        1 tttgttttta tatggcagga tccttcaggt tattgcgtat gtttttattg gatgttctgc
       61 ttattattga tgagtaatag cattataatt aagttcatat tgttaacatc gcttatcaat
      121 tatttttatg tcgataacac tgtgtcgtta tgcatgaaga gtggcagggc agcatcgtta
      181 aaaatacaag agttaacaga aacactttta tcgtggcgca catatatcct ccacatatta
      241 taaaaaataa aaagaccacc atcagaagtg ctggcctttt gtttatcata tttaaagtgt
      301 catgaaaaaa ttaaaaacag gcatgtttca ggttaaaaat aatacttcag accagctgtg
      361 gtcataaagt tatagctttc aatgcctgca ccatttttgg tgtgatcaga tatattcaaa
      421 tttctggagt acagagaagt atcacctttc ttattagtaa tacggttcca tgttccttcg
      481 acataaactt tagctgcagg tgtaatataa taaccagcat gtagtgagac agagtaatag
      541 ttctgattat ttacatcgct acgataggtg atccttttct cagggttgta atgctcatca
      601 ttatccgatg ccttcaccca tccgctatat ttgaaactcc caccgaactc aaagctatca
      661 tacagataat taccggttaa ccctatatag ggcattttaa agtgttgttt gtagcctata
      721 gctctttcac catctggaaa tgatcccgtt tcatcccgga agcctccctc actactatag
      781 atataagagc ctccttttgc tgtaaagcta taacgatttt cctggtaacc agccataaga
      841 cctaactgat agtctggttg attcaggagc catcccttaa tattcaggtc gaattcatta
      901 gcaaaattta gacgggtatt cggatgttta ctctcatcag tccaggttcc gggattgctc
      961 gtatccagcc agtccctgtc caccatattg cctccacgac cagctaatgt tgtccaccct
     1021 gaggcaccga cagatactct gggtaacaga tcccagttaa atgccccctt gacaattggc
     1081 gcattactgt atttccagtc cagctggctg gctttgcgcc ctttttcttc aggcagataa
     1141 acccgttctt tggccttgcc acttaaagtg ccaaaatcaa tctccgtact gattttctcc
     1201 ggtgtaaaag aaagcccagt atcagaagcc agtgcagcaa acgctacagg agctgagagt
     1261 gcagtagcaa gaatctttaa gtacatagac aagttaactc cattccactc gtttgttttt
     1321 gataaatact tttatttact cgtagtagtc aactgaaaaa tgctgttttg tttaacgttt
     1381 gtttacaatg tggccgcatg tcgctttatt tattcccttt tatgtcagaa tatcagggga
     1441 gtcagtcaaa cccggtatga ttactgcccc ggtcatccat gttccaggaa atatcagggc
     1501 atacagggtg ctggtaactg cgaataaggc cctggtcgga cactttatgc cgggaacgtc
     1561 tgtaacatgg aaaccggtac cttcttcctg ggtttatttt gttaactctt atctaaaggg
     1621 agacaaaatg gccaaatcct gcgattatga agcaatcagg ggctttatta gcgttaatgt
     1681 cggcaaagat accaataacg ccattacagt taatta
//
LOCUS       DBBGLQ010000322         1701 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.328, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000322 DBBGLQ010000000
VERSION     DBBGLQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.328"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..503
                     /gene="wzy"
                     /locus_tag="ACIYSB_005631"
     CDS             <1..503
                     /gene="wzy"
                     /locus_tag="ACIYSB_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158642101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O166 family O-antigen polymerase"
                     /protein_id="HGC5432407.1"
                     /translation="KFKFIYTAKFYFSSIVVALLLFKINIFGVLINTGYLPHQITRYI
                     NQDEMMGNTFGFTAIITIVVSFLMCLRFREYYGIDYKLLYLNMLFSSITALVFMNIPI
                     MMRMQLLCLTAIIILPNVIYTYWIRKDSANRLLLKIFFVSVCIIYYYSVLSTGIVSGY
                     KTFFSI"
     gene            514..1596
                     /locus_tag="ACIYSB_005632"
     CDS             514..1596
                     /locus_tag="ACIYSB_005632"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGC5432408.1"
                     /translation="MKNICFFVGDISRSGGTERVTCKLANYLTLNGFDVSIISIYGTN
                     SAFFPLDEKVKKYKLFDRYLKGTLSFPLIIFRLRKLLKKKCIHKLISVESMLALYSLP
                     ATFNLPIENICWEHFNFKINLGKKTRNIARRMAAEYCSYVITLTDRDKAYWENNLKCK
                     AIIKRIYNPIPFDNMYLPKFEKKKRIAIAVGRLTHQKGFDVLIKIWADLYHYNANWTL
                     KIIGGGEEEHSLKTFCQNHDISNIEFISVTDNIQDYYSEASLYLMTSRYEGFPMVLLE
                     AQEYGIPVLAFDCETGPSELILHNKTGWIINNNNVREFEERLIYALNLFEKDHDTYNK
                     MCHEAFLNSKNYHIENIGSQWINILK"
BASE COUNT          598 a          206 c          311 g          586 t
ORIGIN      
        1 taaaatttaa attcatttac actgccaaat tttatttttc gagcatagtg gttgcattat
       61 tgttatttaa aattaatata ttcggcgtgc taattaatac aggatatctc ccacatcaaa
      121 taacaagata tattaatcag gatgagatga tgggaaatac tttcggcttt actgcgataa
      181 ttaccattgt tgtttcattt ttaatgtgct tacgatttag agagtattat ggtattgact
      241 ataaacttct atatttaaat atgttgttct caagtattac agctttagtt tttatgaata
      301 ttccaataat gatgagaatg cagttgttat gccttacagc aataattata ttgccaaatg
      361 taatctatac ttattggata agaaaggatt cggcgaatag acttttactg aaaatattct
      421 ttgtgtctgt ctgtattatt tattattact cagtgttatc aactggaatt gtatcagggt
      481 ataaaacatt ttttagtatt taagagggtt attgtgaaaa atatttgttt tttcgtcgga
      541 gacataagcc ggtcgggggg gacagaaaga gttacatgta aattagctaa ttatcttaca
      601 ttaaatggct ttgatgtttc tatcataagt atttacggta caaatagtgc cttttttccg
      661 ttagatgaaa aagtaaaaaa atataaactc tttgataggt atttaaaagg aactttaagc
      721 tttccattga taatatttag actaaggaaa ttattgaaaa agaaatgtat acataaacta
      781 atttcagttg aatcaatgct tgctttatac tcgctaccag caacttttaa tctaccgatt
      841 gagaatattt gttgggaaca ttttaatttt aaaattaatt taggaaaaaa aacgagaaat
      901 atagctaggc gaatggccgc cgaatattgt agctatgtca ttacattaac agacagagat
      961 aaagcttatt gggagaataa cttaaagtgt aaagcgataa taaaaagaat ttataatcct
     1021 atcccctttg ataatatgta tttgccaaaa tttgagaaaa aaaaacgaat agctatagct
     1081 gttggtcgat taactcacca gaagggattt gatgtgctaa ttaaaatttg ggcggactta
     1141 tatcattata atgccaactg gactttaaaa ataataggtg gcggtgagga agaacacagt
     1201 ttaaagacat tttgccaaaa tcatgatatt agcaatatag aatttatttc cgtcacagat
     1261 aatatacagg attattatag tgaagcctca ttatatttga tgacatctag atacgaaggg
     1321 tttccgatgg tacttttaga ggcgcaggaa tatggtatac ctgtattagc ttttgattgt
     1381 gagactggcc ctagtgagtt aattttgcat aataagactg gttggataat aaataataat
     1441 aatgtgcgtg agtttgaaga aagattaatt tatgcgttaa atttatttga aaaagatcat
     1501 gatacctaca ataaaatgtg tcatgaagca ttcttaaact caaagaatta tcatatcgag
     1561 aatataggtt ctcagtggat taatatcttg aaataaaggg aataatatga aaaatatttg
     1621 tcttattaca accggccttg gtatgggggg ggccgagaga caagtttgtg acctggcaaa
     1681 tagtttctat gataagggaa a
//
LOCUS       DBBGLQ010000323         1695 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.329, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000323 DBBGLQ010000000
VERSION     DBBGLQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.329"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..544
                     /locus_tag="ACIYSB_005633"
     CDS             <1..544
                     /locus_tag="ACIYSB_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="HGC5432409.1"
                     /translation="APIVGLLLGMSSISVPWDTLLISVVLYIIVPVILAQLLRRHLLK
                     QGQAAFERAMQKIGPWSMAALLLTLVLLFAFQGEAIIRQPLVIAMLAVPILIQVFFNS
                     GLAYWLNKRAGEKHSVACPSALIGASNFFELAVAAAISLFGLHSGAALATVVGVLVEV
                     PVMLLVVRVVNRSKSWYERG"
     gene            659..862
                     /locus_tag="ACIYSB_005634"
     CDS             659..862
                     /locus_tag="ACIYSB_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432410.1"
                     /translation="MTDPKNLENRLHEKADPARAVSTGRVRYTLAELLADAEASGAYP
                     LPPEEREWMDAPSVGREWPNSED"
     gene            complement(899..1123)
                     /locus_tag="ACIYSB_005635"
     CDS             complement(899..1123)
                     /locus_tag="ACIYSB_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system ptaRNA1 family
                     toxin"
                     /protein_id="HGC5432411.1"
                     /translation="MTTQHIIEPGQAVHQAAAILSSLEYINQAEARSLGPLAEAVANA
                     FMVVYYQAETGRATQADFQEAMNALRQACS"
     gene            complement(1187..1435)
                     /locus_tag="ACIYSB_005636"
     CDS             complement(1187..1435)
                     /locus_tag="ACIYSB_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432412.1"
                     /translation="MTTSTYMAALAALDEAPREEVSQVFISPLGDQSTEVDAETETAA
                     FVSRPDPMRESETKPISIEVNGGRNERSAFAEAAGLYL"
     gene            complement(1440..>1695)
                     /locus_tag="ACIYSB_005637"
     CDS             complement(1440..>1695)
                     /locus_tag="ACIYSB_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HGC5432413.1"
                     /translation="GSSSSGGGFAKAAKLATGTASELAKGVGSQVKQGFQERVSETTG
                     GKLAASIRESMEPKEASQSGQFEGNSLGADSGPDSNEVRS"
BASE COUNT          303 a          506 c          487 g          399 t
ORIGIN      
        1 cgcgcccatc gtcggcctgc tgctgggcat gtcctcgatc tcggtgcctt gggacacgct
       61 gctgatttcg gtcgtgctct acatcatcgt gccggtgatc ctggcgcagc tcttgcggcg
      121 gcatctgctc aagcaaggac aggccgcctt tgaacgggcc atgcagaaga tcgggccgtg
      181 gtcgatggcc gcgctgctgc tgacgttggt tctactgttc gccttccagg gcgaggccat
      241 catccggcag ccgttagtga tcgccatgct agccgtgccg atcttgattc aagtgttctt
      301 caattccggg ttagcctact ggctgaacaa gcgggcgggg gaaaagcact ctgtcgcctg
      361 tccttcggcc ctgatcggtg ccagcaactt cttcgagctg gccgtggcgg ccgccatcag
      421 cttgttcggt ctgcactccg gcgctgcatt ggcaaccgtg gtcggcgtcc tggtcgaagt
      481 gccggtgatg ctgctggtcg tgcgcgtggt gaatcgctcg aagagctggt acgaacgagg
      541 ttgaacatga gtaggaaaaa actgaggaaa tgattgcaga ttgccgcgcc ggccgcttcc
      601 tggcgcattc gatcactgac ctggaacacc tagtatccat gcttgctgag gtcgcccgat
      661 gacagaccca aagaacctag agaacaggct gcatgagaag gccgacccgg cccgcgccgt
      721 ttccactggt cgggtgcgct acacactggc cgaactactg gccgacgcgg aagcatccgg
      781 ggcttacccg ctgccgccgg aggaacgcga atggatggat gcgccgtctg tgggtcggga
      841 atggccaaac agtgaagatt gaaaaaggcg gccagttcga gtggccgccc tttcgtcatt
      901 agctgcacgc ttggcgcaag gcgttcattg cttcttgaaa atcagcctgt gtcgcccggc
      961 ccgtttcagc ttggtagtac accaccataa aagcattagc gacggcttcg gccaatggcc
     1021 caaggctccg cgcttccgct tggttgatgt actccaaaga agaaagaata gccgctgctt
     1081 gatgcactgc ttgccctggt tcgataatat gttgtgttgt cataattcac ctctccagta
     1141 ggtgttgatt gaaagtgccg ggctggttgt ggccagcccg gtgcgcctac aggtacagcc
     1201 ccgccgcctc cgcgaatgct gaccgctcat tcctgccgcc attaacctca atacttatcg
     1261 gcttagtttc gctctctctc atagggtctg gccgtgatac aaaagcggct gtttctgtct
     1321 ctgcatcgac ctcggtcgat tgatcaccca gcggcgaaat aaatacctgg ctgacttctt
     1381 cccttggcgc ttcatccagg gccgccaatg cggccatgta tgttgatgtc gtcatcgtgc
     1441 taactcctga cttcgttact atctgggcca gaatcggcgc ccaagctatt gccctcgaac
     1501 tggccagatt ggcttgcttc tttcggctcc atgctttcgc gtatcgaagc agccagtttt
     1561 ccgcctgtgg tttcgctcac tcgctcctgg aatccctgct tcacttgaga gccgacaccc
     1621 ttggctaact cggaggccgt gcctgtggcc agcttcgcgg ccttggcaaa gccgccaccg
     1681 cttgagctac tgccg
//
LOCUS       DBBGLQ010000324         1691 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.330, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000324 DBBGLQ010000000
VERSION     DBBGLQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.330"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..308
                     /locus_tag="ACIYSB_005638"
     CDS             <1..308
                     /locus_tag="ACIYSB_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGC5432414.1"
                     /translation="KEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTL
                     KANDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            369..>1691
                     /locus_tag="ACIYSB_005639"
     CDS             369..>1691
                     /locus_tag="ACIYSB_005639"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HGC5432415.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGLIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAV"
BASE COUNT          395 a          372 c          406 g          518 t
ORIGIN      
        1 tcaaagaggt aacacagaat tttgctctgg cctttgaggg tagctatcaa tatatggatc
       61 tgaaaccaaa ggggtatcaa aaccacaacg ccgtaaacgg cagcttctat aaactcacct
      121 ttgctccaac tctaaaagct aacgatatca ataatttctt tagccgtccg gagcttcgcc
      181 tgtttgccac ctggatggac tggagcagca aacttgatga ttttgccagc aatgacgctt
      241 tcggcagcag tggtttcaat actggcggag agtggaattt tggtgtccaa atggaaacct
      301 ggttttaata ccagacagcc tcacggatgt gaggctttta ctgaaataac aatgaattag
      361 agggttatat ggatttcgat aaaatcgccc aatcgttgct tcctttatta ggtggtaagg
      421 agaacatcgc cagtgcagca cattgcgcca ctcgcctacg gctggtactg gttgacgaca
      481 cacttgccga tcaacatgcc attggtctga ttgatggagt gaaaggttgt tttcgtaatt
      541 cagggcaaat gcagatcatc tttggcaccg gcgtggttaa taaagtctat gctgccttta
      601 ttcaggttgc gggtattagc gaatccagta aagcagatac cgctcgactt gccgctcaaa
      661 aactcaatcc ttttcagcga atagcacggc tactttctaa tatttttgtc cccatcattc
      721 cagccattgt tgcttctggt ttattaatgg ggcttctggg aatggtgaaa acatatggct
      781 gggtgaatgc cgataatgcg atttatatcc tgctggatat gtgcagttca gccgcattta
      841 ttattctccc catccttatt ggctttactg ctgctcgtga atttggtgga aatccttatc
      901 ttggcgcgac attaggaggg attctgactc acccggcact cacaaatgcc tggggcgttg
      961 ctgcgggatt tcagacaatg aacttcttcg gttttgaaat cgccatgatt ggctatcagg
     1021 gaacggtttt ccccgtgctt ctggcagtat ggtttatgag catagtggaa aaacagttac
     1081 gcaggtttat ccctgatgct ctggatctca ttctgacgcc atttctgact gtcgtcattt
     1141 ctggctttat cgctcttttg attattggtc cggcaggacg agcgttaggt gacggtatct
     1201 ctttcgttct cagcacgctg attgcacacg caggctggtt agcagggttg ttattcggcg
     1261 ggctatattc agcgattgtt attacgggta ttcatcatag cttccacgca attgaagcag
     1321 gacttttagg taaccctgca atcggtgtta atttcctgct gcctatttgg gcaatggcaa
     1381 acgttgcgca aggcggtgca tgtctggcgg tatggtttaa aaccaaagat acaaaaatta
     1441 aagcaatcac cctaccctcg gccttttccg caatgttagg gattactgaa gccgctattt
     1501 ttggtatcaa ccttcgtttt gttaaacctt ttattgcggc cttaattggt ggcgctgccg
     1561 gcggtgcctg ggttgtctct gttcacgttt atatgactgc cgttggtctg accgcaatac
     1621 ccggcatggc aatcgttcag ccaacatcgt tggttaatta tattattgga atggtgattg
     1681 ctttcgctgt c
//
LOCUS       DBBGLQ010000325         1685 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.331, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000325 DBBGLQ010000000
VERSION     DBBGLQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.331"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..812
                     /locus_tag="ACIYSB_005640"
     CDS             <1..812
                     /locus_tag="ACIYSB_005640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGC5432416.1"
                     /translation="FSRILRLIYPKVPLICTAHNTNEGGRLRMFLYRITDFLATISTN
                     VSQEAVESFVQKGAVKRGRMIAFYNGIDTNLYKYSPSYRINKRKELNLNDEDVLLLAV
                     GRLTDAKNYKNMLKALQIVIENKRVHLAIIGNGPDRNKLIDYASRLGITQNVHWLGVR
                     FDVYEWMSAMDLYVMSSSWEGMPLVICEAMSCGGIVVATDCGGVKEIVGNSGFIVPKN
                     DSKALSKSISDALALTEEERVSITSSARDRIERYYSLNIISGKWLSLYDEF"
     gene            799..>1685
                     /locus_tag="ACIYSB_005641"
     CDS             799..>1685
                     /locus_tag="ACIYSB_005641"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGC5432417.1"
                     /translation="MTNSDTSDLYNNQKNIVFVITKSEVGGAQTWVSQLKFILEEKHN
                     IFLITSETGWLTDYFEPEKVKIVPGLVSMLKPGAIFKISSALKEFNADLVISNSATAG
                     LYSRLAKIFYRHKHIYVSHGWSCIYNGGRFEKIFCLIERFLSLLTNKVLCVSINDQYN
                     ATHVIGINADKTVVIRNSINPMEKKNFINKKFKILFVGRMSYPKRPDLLAEAAVYFPD
                     VVFDFVGGGPLLSELKKKYQSSPNIRFLGEIGSFKNFKDYELFVLASESEGLPMSALE
                     AATAGLPLLLSDVGGCNEL"
BASE COUNT          547 a          241 c          348 g          549 t
ORIGIN      
        1 ttttttcacg gatattaagg ttgatatatc caaaagtccc tctgatttgt acggcgcaca
       61 atacaaatga aggtggcaga ctgagaatgt tcttgtacag aattactgat tttttagcaa
      121 caatttcaac aaatgttagc caagaagccg ttgaaagctt tgttcagaag ggggctgtca
      181 aaagaggcag aatgattgca ttttataatg ggatagatac gaatttatat aaatattctc
      241 ccagttatag aattaataaa agaaaagaat taaatttaaa cgatgaggat gtattattac
      301 tagccgttgg tcgattaact gatgcgaaaa attataaaaa tatgctaaag gcactgcaaa
      361 tagttattga aaataaacgt gtgcatctcg ccattatagg taatggtcct gatagaaata
      421 aactcataga ttatgcttct cgattaggaa taacccaaaa tgtgcattgg ttaggagttc
      481 gttttgatgt ttatgaatgg atgtcagcga tggacttgta tgttatgtct tcttcatggg
      541 aaggtatgcc tttagttata tgtgaggcta tgtcttgtgg cggaattgtt gtagcgacag
      601 attgcggagg agttaaagaa atagttggca attctggctt tattgtgcca aaaaatgatt
      661 ccaaagcatt atcaaaatca atatccgatg cattagctct tacagaggaa gagagagttt
      721 caatcacatc ttctgcaaga gataggattg aacggtacta ttccttaaat attatttcgg
      781 gaaaatggtt aagtttatat gacgaattct gatactagcg acctgtacaa taatcaaaaa
      841 aatattgtgt ttgttattac taagtctgaa gtcggtgggg cccaaacttg ggtttcacaa
      901 cttaaattta tacttgaaga gaagcataat atttttctaa taacttcaga gactggatgg
      961 cttacagatt attttgaacc tgaaaaagta aaaattgtac ccggcttagt cagtatgtta
     1021 aaaccggggg caatttttaa gataagttct gcattaaaag agttcaatgc agaccttgtg
     1081 atatctaatt ctgctactgc tggactgtat tctcgacttg caaaaatatt ctataggcat
     1141 aaacatatat atgtatctca tggatggtct tgcatatata atggagggcg gtttgaaaaa
     1201 atattttgcc tgatagaacg ctttttatcg cttttgacaa ataaggttct atgtgtttct
     1261 ataaatgatc aatataatgc aacccatgtc attggaataa atgcagataa aaccgttgtc
     1321 attagaaact cgattaatcc tatggagaaa aaaaacttca ttaataagaa atttaaaatt
     1381 ttgtttgtag gccgaatgtc ttatcctaaa agaccggact tgttagctga ggctgcggta
     1441 tactttcctg atgtggtttt tgatttcgtc ggtggtggtc ccttattatc agagctaaaa
     1501 aagaaatacc aatctagccc taatattaga tttttgggtg aaatcgggag tttcaagaat
     1561 tttaaagatt acgaattgtt tgtattggcc tcagaaagtg agggcttacc tatgtcagct
     1621 cttgaggccg ctactgctgg attaccatta ttattgagtg atgtcggcgg atgcaatgaa
     1681 ttaat
//
LOCUS       DBBGLQ010000326         1669 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.332, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000326 DBBGLQ010000000
VERSION     DBBGLQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.332"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            76..1155
                     /locus_tag="ACIYSB_005642"
     CDS             76..1155
                     /locus_tag="ACIYSB_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGC5432418.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSDAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTQAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            1155..>1669
                     /locus_tag="ACIYSB_005643"
     CDS             1155..>1669
                     /locus_tag="ACIYSB_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGC5432419.1"
                     /translation="MKAIETIQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNSPKARFEMDLEVTGQVKDLCDSSGTTMSAMRLAYN
                     GHRHRENGQGS"
BASE COUNT          364 a          422 c          544 g          339 t
ORIGIN      
        1 cgcggacatt atccggcgta atgccattac gcatcccggc tttgtgccgg tgatccctct
       61 gaaggtgcca gtgcaatgaa cgacaatgtc acgctacggg taaatggccg ggagtggaat
      121 ggctggacat cggtgcgcat cggtgccggt attgaacggc tggcgcggga tttcagcgtg
      181 gagatcaccc gccagtggcc gggtgatgag ggtatcacca cgcttcagcc gcgcattaaa
      241 aatggttcaa aagtggaagt gctgattggt gatgagctgg tgatcaccgg ctgggtggag
      301 gcgacccccg ttcgttacga tgcccgttcg gtcagcaccg gtattgccgg acgtagtctg
      361 actgctgacc tgattgactg tgcagccgaa ccgacacagt ttaacggacg atcgctggta
      421 cagattgcgc aggcgcttgc tgcgcctttc ggcattgagg tggtgaacag cgatgcgccg
      481 tcgggtgtta ttccggatgt ccagcctgat cacggtgaaa cggtgatcga ggtgatcaac
      541 aaaatactcg gtcagcagca ggcgctggct tatgacgacc cgcacggcag gctggtgatt
      601 ggtggtattg gctcaacgca ggcacatacc gcgctggtac ttggggaaaa catcctttcc
      661 tgtgatacgg agaagagtat ccgggagcgg ttttcagttt accaggtggc ggggcagcgt
      721 gccggaaacg acgatgattt cggtgaggcc accaccaccg cgctgcgggc ccgcacagag
      781 gatgcattta ttgcccgtta ccgtccgatg tatatcaggc agacagggca ggctacgggg
      841 gcaggctgta ttgcgcgtgc tgactttgaa gcccgacaac gggcggcgcg gacggatgaa
      901 accacctatg tggtgcaggg ctggcgacag ggtaacggta cgctgtggca gcccaaccag
      961 cgggtgattg tcttcgatcc ggtctgtggt ttcgacaata ccgaactgct tgtctcggaa
     1021 gtcacgttta ctcaggacca gaatggcacc ctgacggaaa tccgtgtcgg cccacctgat
     1081 gcttatctgc ctgaacccga agcccccggc gcgcggaaaa agaaaaaagc cagagtacag
     1141 gaggacccgt tctgatgaag gcgattgaaa ccatacagcg acaactcctc ggcctgattg
     1201 ggcgggcggt ggtgaaaagc atcagtgccg ccacgaaatg tcagaccgtg gatgtgtccc
     1261 tgattgccgg tgaacccaaa gccggggttg aacatcttga accctacggt tttaccgcaa
     1321 gagcaaacag cggtgcggaa gcggtggtgt tgtttccgga tggtgaccgt tctcatgcgg
     1381 tggttgttac ggtgtcggac cggcgctacc gcctgaaagg gctgcagact ggtgaggtgg
     1441 ctgtctatga cgatcagggg cagtccgtga cgctgacccg ggaggggatc gtggtggacg
     1501 gtgcaggtaa aacgatcacg tttcgcaatt cacctaaagc acgttttgaa atggacctgg
     1561 aagtgacagg acaggtgaaa gacctgtgcg actccagcgg caccaccatg tcagcgatgc
     1621 ggcttgccta taacgggcat cgtcacagag agaacggtca gggcagtaa
//
LOCUS       DBBGLQ010000327         1636 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.333, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000327 DBBGLQ010000000
VERSION     DBBGLQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.333"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            328..564
                     /locus_tag="ACIYSB_005644"
     CDS             328..564
                     /locus_tag="ACIYSB_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432420.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFK"
     gene            734..1555
                     /locus_tag="ACIYSB_005645"
     CDS             734..1555
                     /locus_tag="ACIYSB_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGC5432421.1"
                     /translation="MLLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
BASE COUNT          462 a          344 c          417 g          413 t
ORIGIN      
        1 cctgcccgtt gttgtgtccg gtgccattca cctgtttaag cccctgtcgc cgggagctga
       61 tacagccaca acctgccgca ctgactgtcc agggtaaatg aacagcccac aaaatttttt
      121 acccaaaacc cggtaaaaaa ttttgcgttc tgcccaggac aggtgcgtca ggccgtggca
      181 gtgatgcccc ttgcggcgaa ggttctcaaa caggtgatgg tgaataccgg agggaacaaa
      241 cggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa actcaacaga tgatgggggc
      301 tgaaaaccag agagaggaga aagcaggatg agtacccgaa atatccacgt taacaccgcg
      361 tcgtacacgc ttcttgttgc cgggaagaaa aaaaacaccg gggaagagtg ggacgtcctg
      421 gaattcagca gcctgactga gctgaaaaaa tatcgcaaaa gccacccgga aaagatggcc
      481 ttcagttata gctacgcgct cagtcagggt gtggataagc agttccgcca tatcaacatt
      541 gcggaagccg atcacttcaa atagttcctg cgtcagatta agcgtgccgg cctcgatatc
      601 cgggcgatct gctgaccgga tgtgatgaat atccctcctg attgttgcaa tcttgttaat
      661 ggtggccggg agggatgata ttaagcggaa aaatttttta ttaaatttat tctttcataa
      721 aggagtagct gttatgctat tagcttcccg ttttggtcgg tataattcca tccgccgtga
      781 acgtccttta acggatgatg aattaatgca gttcgtgcct tcggtatttt ccggtgataa
      841 acatgagtcc cggagtgaac gttatacgta tattccaaca atcaatatca tcaataagtt
      901 acgtgatgaa ggtttccagc cattctttgc ctgtcagagt cgggttcgtg atttgggacg
      961 tcgcgaatac agtaaacata tgttacgtct tcgcagggaa gggcatatta acgggcagga
     1021 agttcctgaa attatcctgc ttaattcaca tgatggttca tccagttatc agatgatccc
     1081 cggaattttt cgttttgtct gcacaaatgg cctggtgtgc ggaaataatt ttggcgaaat
     1141 ccgcgttcca cataaaggtg atattgtcgg gcaggttatc gagggagcgt atgaagtgct
     1201 cggtgtcttt gataaggtca ctgataatat ggaggcgatg aaagaaattc atcttaacag
     1261 tgacgagcaa catttatttg gcagagctgc actgatggtc aggtatgaag atgaaaataa
     1321 aacgccagtg acacctgaac agataattac tccccgtcgt cgggaagaca aacagaacga
     1381 tctctggaca acctggcagc gggttcagga gaatatgata aaaggtggat tatcggggcg
     1441 aagtgcctcc gggaaaaata ccaggacaag agccattaca ggtattgatg gtgatataag
     1501 aatcaacaag gcgttatggg tgattgccga acagttcaga aagtggaagt catgataata
     1561 taagtaaagc cccgaaaaaa ttttcggggc tttacttata ttatcatttt tcggcattgc
     1621 tgtgtcgtct gatata
//
LOCUS       DBBGLQ010000328         1631 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.334, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000328 DBBGLQ010000000
VERSION     DBBGLQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.334"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..477)
                     /locus_tag="ACIYSB_005646"
     CDS             complement(<1..477)
                     /locus_tag="ACIYSB_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGC5432422.1"
                     /translation="MNRLRFEVKNNQGCFIFPETWYESLLEKFNELLDSFDEDEISDR
                     SYINALKRITQQEPDFIDAYAYMAYAFLEQETPRKALNIALKGLAAGNRLIPEGFTGE
                     ISWLHPENRPFLNALYAAILANVHLQHHQNAVMLIDKILAYNPSDNPGARWLLGPEL"
     gene            complement(844..>1631)
                     /locus_tag="ACIYSB_005647"
     CDS             complement(844..>1631)
                     /locus_tag="ACIYSB_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5432423.1"
                     /translation="NRPVTGIRPMEILNVLRTIEEQGKLEALRKTRQLCAQVFDYAIA
                     TGRATVNPASTLSGVLLPPEKQQRRTLSEEQIGAFMYQITASGNLMSLATRMLVLTAV
                     RTGELRLATWDEINFTEALWVIPKERMKMRRPHVVPLSRQVLALLEEIKTRTESRNSA
                     FIIPGAFRKDLPRNRKCINEFLEQSGWHALTTAHGFRHTFSTILHDHDFSSAWIELQL
                     AHVDKNVMRGTYNHAQYLDGRREMMQWYADYIDSLMEKNVKTK"
BASE COUNT          401 a          376 c          396 g          458 t
ORIGIN      
        1 cagttccggt cccagtaacc agcgtgctcc cggattatct gaaggattgt aagccagaat
       61 tttatcaatg agcatgacag cattctgatg atgctgcagg tgcacattcg cgaggatggc
      121 ggcgtaaagg gcattcagaa agggcctgtt ttccggatgt aaccagctga tttcaccggt
      181 gaaaccttca ggtatcagtc ggttacctgc cgccagtcct ttgagtgcaa tattcagcgc
      241 ttttcgcggt gtctcctgct ccagaaaggc atatgccatg tatgcatacg catcaataaa
      301 atccggttcc tgttgtgtga tacgcttcag ggcattgata taagacctgt cgctgatctc
      361 atcctcgtca aaggagtcaa gcagttcgtt aaacttctcc agtagtgatt cataccaggt
      421 ttccgggaaa atgaagcagc cctgattatt cttcacttca aagcgtaatc tgttcatatg
      481 ctgaatctgt tgtaaatctg gaaaaactta tagttctcca agaataaagc ccacggcagt
      541 ttgtatcaag tttcttctca caccggtaac agccggccag agctattttt gtgttagtca
      601 tggattcatt ctttcaatga ggagcaatca gcctgactgg taatgatgct gtagaaatgt
      661 gactacgagg tcagaacagc agtgtgctgc tatatgaaaa tgacgatgtg aagctaccat
      721 cattttgggt ggggtatctt cgctcatcag atgatattga gtatgtcgcc tgaaggcaga
      781 aatcaaaaaa tacaaatggt agttaactcc ccaccaaccg ctgtggttgg tgggggtgat
      841 ggcttatttc gtttttacgt ttttctccat aagtgaatca atgtaatcag cgtaccactg
      901 catcatttca cgacgtccat ccagatattg cgcatggtta taagtgccac gcatgacgtt
      961 tttatcaaca tgagcaagct ggagttcaat ccaggctgag ctgaaatcat ggtcatggag
     1021 aatggtgctg aaggtatgac gaaatccgtg ggctgttgtc agggcatgcc agcctgactg
     1081 ctcaaggaat tcattgatac acttgcggtt tcgcgggaga tcttttctga atgcaccagg
     1141 aataataaag gcggaattac gcgattcagt gcgtgttttt atctcctcca gtaacgccag
     1201 tacctgccgt gaaagcggca caacgtgcgg tcgtcgcatt ttcattcttt ctttcggaat
     1261 aacccacagg gcctccgtga aattaatttc gtcccaggtt gccagtcgca gttctccggt
     1321 gcgaacagcg gtcagaacca gcatccgcgt tgccagcgac atcagatttc cggaagccgt
     1381 tatctgatac atgaaagctc ctatctgttc ttcagaaagc gtcctgcgct gttgtttttc
     1441 aggaggaagc agtaccccgc tgagtgtcga tgccggatta acggttgcac gccctgttgc
     1501 tatggcataa tcaaaaacct gcgcacagag ctggcgtgtt ttacgcagtg cttcaagttt
     1561 tccctgttct tctatagtcc tcaggacgtt cagtatttcc atggggcgga tcccggtcac
     1621 aggacggttt c
//
LOCUS       DBBGLQ010000329         1630 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.335, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000329 DBBGLQ010000000
VERSION     DBBGLQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.335"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            88..1104
                     /locus_tag="ACIYSB_005648"
     CDS             88..1104
                     /locus_tag="ACIYSB_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISShdy1 family transposase"
                     /protein_id="HGC5432424.1"
                     /translation="MTVVTVGIDLAKNVFAVHGVNAAGKAVLVKPLVRRAKLLELIAT
                     LPACLIGIEACTGAHYWAREFQKMGHTVRIMAPQLVSPYRMGGRHGKNDAADAAAICE
                     AVTRPNMRFVPIKSVEQQGQLFVHRAHQGYVSERTALINRIRGLLSELGIVLPQAFKA
                     FLSGVAQVLEDLPGYCNQVIGDLLNELYHIEEKIKNSEKLIQQLARDNSAAQLLMQFR
                     GIGPMTATAIVASIGNGHDFKNGRQFSAWLGLTPRQQSSGGKARLGRITKAGDSYLRT
                     LLVLGARSVLLGAAKNDDPVSRWALQLKERRGYGRALVAIAAKNARMCWAMLHLRDEF
                     RFPA"
BASE COUNT          425 a          315 c          409 g          481 t
ORIGIN      
        1 ttattcggtc tttacgctgg agagttactt tggaagactg cgtcaacacg atgataagcg
       61 tacaaagcca ttgttaaggg atcctctatg actgttgtta ctgtcggtat tgatttagct
      121 aaaaatgtgt tcgctgtgca tggcgttaat gctgcaggaa aagctgtttt agttaagcct
      181 cttgtgcgtc gggctaaatt gcttgaattg attgcaacac ttccagcatg tctgattgga
      241 attgaagcat gtacaggtgc acattactgg gcaagagagt tccagaaaat ggggcataca
      301 gttcgaatta tggcgccgca actggtttct ccataccgta tgggtggccg tcatgggaaa
      361 aatgatgctg cagatgccgc tgccatttgt gaagctgtta cccgtcctaa tatgcggttt
      421 gttcccataa aatcagttga gcagcaaggg cagttatttg tacacagagc tcatcaggga
      481 tatgtcagtg agagaacagc attgattaat cgcatccggg gattgctcag tgagttaggt
      541 attgttctcc cgcaggcgtt taaagcgttt ttatccgggg tggctcaggt ccttgaggat
      601 ttgcccggtt actgtaatca ggttattggt gatcttctta atgagcttta tcacatagaa
      661 gaaaagatta aaaacagtga aaaactcatt cagcaacttg ccagagataa ctcagcagca
      721 caactactaa tgcaattcag ggggataggc ccgatgactg caacagcaat cgttgccagc
      781 ataggtaatg gtcatgattt caagaacgga cgtcagttta gtgcctggct ggggctaaca
      841 ccaagacaac aaagttccgg ggggaaagca cggttaggga gaataactaa agccggcgac
      901 agctatctca gaacacttct tgttttgggg gcgagatctg tattgttggg agcagctaaa
      961 aatgatgatc cggtcagtcg atgggccctg cagttaaaag aaaggcgagg atatggacga
     1021 gcgcttgttg cgatagcagc aaaaaatgca cgtatgtgct gggcaatgtt gcacctgaga
     1081 gatgaattca gatttcctgc ctagtctact gattattaat gagtttatga aattgatttc
     1141 tttccttgca tgacgactgc acgatgatga tttaaaaggt ttggacctgc gccatggttt
     1201 actcgagata tgcaagaggc tgccatgttt atgtggatat ctgtttcgcg gatggagtct
     1261 atggcgtgcg tctttcgtca gggttcgttg ctaacaacag cacgttaata accgtttata
     1321 ggctcgctgt ccgtaacctt ttactctgac tgtgcaagtt gtgtgcagaa atcaatctca
     1381 tgatagataa aagaattatt ccactgttgc acctggagtg ttcggtgctc gctttgctgc
     1441 gctccgcctc aatccagttg caacagcgtt taattaaaag cattaaggct tgtgtcattg
     1501 cgtacttgag ctatgcagga gatttttatt ggttctctgt ttcgcggatg aagtctctga
     1561 catgagcctt tctctcataa aaatcgttct atttcatatt gatatattaa tttcatcatc
     1621 atcgttgact
//
LOCUS       DBBGLQ010000330         1612 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.336, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000330 DBBGLQ010000000
VERSION     DBBGLQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.336"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            68..406
                     /locus_tag="ACIYSB_005649"
     CDS             68..406
                     /locus_tag="ACIYSB_005649"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HGC5432425.1"
                     /translation="MKELPVTLLYSESLFRTIKYCSWWPENGFRTIDEARSWLSKFTQ
                     WYNLEHKHSGIKYVTPDERHRGIDAQILEAGKKVYREARKRHPERWSKQLRDWELIQA
                     VYLNPEKEAT"
     gene            894..>1612
                     /locus_tag="ACIYSB_005650"
     CDS             894..>1612
                     /locus_tag="ACIYSB_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HGC5432426.1"
                     /translation="MKKVFAVSVVAAACVFAANAGAEEGKSGFYLTGKAGTSVVSLSD
                     QRFLSGDEEETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWISAGIGYARIHQKTTG
                     ISTWDYEYGSSGRESLSRSGSADNFAWNLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIML"
BASE COUNT          421 a          350 c          403 g          438 t
ORIGIN      
        1 cttttgttcg ggttcacttt cctgtgttgc tctggtgagc agtgccctgc tggttttcac
       61 tgatgcgatg aaagaactgc cagtaacact tctttattcc gaatcgctct tccggacgat
      121 taagtattgt tcatggtggc cggagaatgg ttttcgaacc atagatgaag ccagaagttg
      181 gttaagcaag ttcacgcaat ggtacaacct ggaacacaag cacagcggta ttaagtatgt
      241 tactccggat gaacgccatc gcggaattga tgcacaaata ctggaagccg gaaaaaaagt
      301 ttatcgcgaa gctcgcaagc gtcatcctga gcgctggagc aaacagttgc gtgactggga
      361 actcattcag gcagtttatc tgaatccaga aaaagaagcg acataacgta attttattac
      421 atcaggcgac aactaccttg aaaaacgccg gaaataactg actctttacc cgctgccatg
      481 cagtgggctt ttttatatcc ccccttcaca aactctgctc actggttata ccggttcacc
      541 tttccggctt gtaaccgcat gcactctgga ctgccagtca gctcatgtat gtctcttcct
      601 taattgtgtt catgaacagg tgacacctta tctcctgaca gaataaacaa attttattaa
      661 ttctgctctt cgccagccca ctgctaccat ctgtcgcgac ggggcatggc tgcctgcatg
      721 gcatgcattt gttgtgtctg tgctgactga acacgataaa attttccggt taatgacgat
      781 cgatatcatc gatcgattcg atcgatttta ttcgattgag gctatcgcat ggcgggtttt
      841 aaaatgcact gagtactgct tgcagcaacg gagcaataac ggagataatt gaaatgaaaa
      901 aggtttttgc tgtttcagtg gtggccgcag cctgtgtatt tgcagcaaat gcaggtgctg
      961 aggaaggtaa aagcggtttt tatctgaccg gtaaagccgg tacctctgtg gtgtcacttt
     1021 cagaccagcg tttcctgtca ggagatgagg aagaaacgtc aaagtataaa ggcggcgatg
     1081 accatgatac ggtattcagt ggtggtatcg cggctggtta tgatttttat ccgcagttca
     1141 gtattccggt tcgtacagaa ctggagtttt acgctcgtgg aaaagctgat tcgaagtata
     1201 acgtagataa agacagttgg tcaggtggtt actggcgtga tgacctgaag aatgaggtgt
     1261 cagtcaacac actaatgctg aatgcgtact atgacttccg gaatgacagc gcattcacac
     1321 cttggatatc tgcagggatt ggctacgcca gaattcacca gaaaacaacc ggtatcagta
     1381 cctgggatta tgagtacgga agcagtggtc gcgaatcgtt gtcacgttca ggctctgctg
     1441 acaacttcgc atggaacctt ggcgcgggtg tccgctatga cgtaaccccg gatatcgctc
     1501 tggacctcag ctatcgctat cttgatgcag gtgacagcag tgtgagttac aaggacgagt
     1561 ggggcgataa atataagtca gaagttgatg ttaaaagtca tgacatcatg ct
//
LOCUS       DBBGLQ010000331         1609 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.337, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000331 DBBGLQ010000000
VERSION     DBBGLQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.337"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..101)
                     /locus_tag="ACIYSB_005651"
     CDS             complement(<1..101)
                     /locus_tag="ACIYSB_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGC5432427.1"
                     /translation="MKIVSQNTTALSSATAPGNNQPQLVATPVPDEQR"
     gene            complement(367..1185)
                     /locus_tag="ACIYSB_005652"
     CDS             complement(367..1185)
                     /locus_tag="ACIYSB_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGC5432428.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHHPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(1275..1508)
                     /locus_tag="ACIYSB_005653"
     CDS             complement(1275..1508)
                     /locus_tag="ACIYSB_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGC5432429.1"
                     /translation="MSDCHPVLLPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
BASE COUNT          366 a          422 c          444 g          377 t
ORIGIN      
        1 cgttgttcat cagggacggg agtagcaacc agttggggct ggttattccc tggtgctgtg
       61 gctgaagaaa gagcagttgt gttctgagaa acgattttca tgagtgaaat ccttacaatg
      121 tgtataaatg agaggaaata aaggccgggg gctgacaaca tcacgttcat cactgaccgt
      181 gagacccggc aatatcaggg atgaatgata ttcggctgaa attccggaca gccgtacgcg
      241 taaccgttcc ggtcacagac ggatggccag aagatgccat tgcctgtact cagtctgcac
      301 agactgagtt ctccggggcg tcgggtgatt ctggtgcctg aatcacgcct tcagggacag
      361 attgcatcag cgcaggctct ccagtagcgt ttctgccatc acccacaatg cgcgattgag
      421 cttaatgtcg gtgtcgatgc tgtgaatggc acgggtgtgg atacgttttc ctctggcact
      481 gcgaccggaa attccgcctt tcagcatatt ctcctggatg gtctgataag tactccacag
      541 gtccttaccg taatcctccc ggcgtcgtgg cgtcagaatg tcggcggtgg tgacggggtg
      601 atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg
      661 cggcaggacc agcgactgca tggcatcacg cttctcctct atccggtcaa acacgcccac
      721 cacctcgtaa gccccttcaa taactttctc caccacattt ccccggtgtg gaacacgcaa
      781 ttctcccaga gactgaccgc agaccagtcc attggtacaa atggcacgaa aatatccggg
      841 taacatctgg tagctggatg aaccgtcatg ggagttgagc agaataattt caggcacatg
      901 ctgaccggtt atctgtccgg cccgccgcag acgcagcata tgtttggtat attcccggcg
      961 gctctggtcg cgcacacggg tctggcaggc gaagaacggc tgaaagcctt cccgctgcag
     1021 attttccagg acggtgatgg tgggaatgta cgcataccgt tcactgcggg aggtgtgccg
     1081 gtcttctcca aaaatactcg ggacgtggta catcagttct tcgcgtgtca gtgggcgctc
     1141 ccgacggatc tgatttacat gaccaaaacg actggctaat tgcatacttt gctccttatg
     1201 aatgaattag cggtataaac gaaaaggccg cgtctcccgg agaagacgcg gcctgatgga
     1261 tgaaatgaag acgtttattg cctgagaatt ccctcactgg cgagatacga atttagctgt
     1321 tttccggcat caggttcaaa attccagcac cgccagcgta gctgcccttc ggcattgcgg
     1381 ataaccagcc ggaaatgcgt tccctggtca tcctcaataa gcacattgcg gtaagctgtt
     1441 gtgacggcaa ccgcctgttc gcgtgagaaa gggccttcgg gcaataaaac agggtggcag
     1501 tccgacatac cggctatttt ccgataaatt caacgctggc gccggtggca ttaccgtcag
     1561 caccaaccgt gacggtcact ttgtcgtacg cacggtcttc aggattgaa
//
LOCUS       DBBGLQ010000332         1599 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.338, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000332 DBBGLQ010000000
VERSION     DBBGLQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.338"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(10..1368)
                     /locus_tag="ACIYSB_005654"
     CDS             complement(10..1368)
                     /locus_tag="ACIYSB_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGC5432430.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVITVIIIQQIIEER
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT          538 a          346 c          253 g          462 t
ORIGIN      
        1 aatgccgctt tatggtgact cacaaaaatt caccacatcc ctcacaacca ccttattacg
       61 cccggtctct ttcgcctcgt aaagtgcgtt atcagccagt cgatagatct catttgggtt
      121 taacgcattt tcctgagtca caacagcgcc aatactaatc gtcacttttt gtggtatagc
      181 gtattcagga ttatcgccag ttaaacgctc aacattttcc cgaatccttt ccgctaaagc
      241 tttcgcacgt tcagtatcga tgtccgtcag caagacgcca aactcttcgc cgccgacgcg
      301 cgctaaaagg tcatctggtc gtatgctttt cccaatgata ttcaccactg tttttatcac
      361 ctgatcgcca accggatgcc cccaggtgtc gttgactttt ttgaagtggt cgatatccat
      421 aatcatgacg caataaggcg ttttttgggc tgatgctgat tgaactgtca gttcattaaa
      481 aaaataattt ctgttaaata tattggttaa gggatcgcga tgtgcaatgt tctttgttac
      541 tcgtagcgca ctgaatatat ggcacataaa tattaccata acggttaact tactgactac
      601 ttcaatagtg cgactaatat accaagtgga cacgctatac ctgctcataa ataacagaga
      661 gatattgtac agtacggcac aaaaagcaat caccgtcact ccattccaga tatcatagcg
      721 taaaccatta aagtatagat taaccgaaag caaggttaac cacatgaaaa tcattatggt
      781 aacatatgag gctttccaaa ccacctgacc attttcattg gttaactcgg caatgtgtaa
      841 attgtagctc tcataatgac tagaaagtat gtgagcaact atcggccccc caaaaacaaa
      901 aaacaaactt atgcacaaag tcattttttt ggaaaataaa ttgcgctgtt ttgtgttttc
      961 actaacttta ttcaccaatg ccagaaatat taatatgcac aaactcatct gacgaaacaa
     1021 ataatagatt gcaatgtcat tttggacaac actgcttgtc aaacgctcct caataatttg
     1081 ctgaatgata atgacggtta taacaaagta aatcaaacaa ctcagaaaag ccaaactaag
     1141 tacacagttt gaaaattgcc tacggtcata aatgaaatac aactgcataa aaataaatgc
     1201 aacagtatcg ataaaaaata aagcaatcat taccatcaga taggttgatc gtggaacaac
     1261 ttcctcaacg cctggctgat taaaccaact attcaccaaa accaaagcaa gcccaaacaa
     1321 taagctcact aatacagtac tactaattct caaatagtct ttttccataa agctacacca
     1381 atctgtaggt tgtagatccc tttctattat taatagatat aagaactgta ctgttgtcaa
     1441 tatgaaattc cgcccacaaa caataccgat gatgatagtt gctgaatgta attattattg
     1501 cctaagtgct ttatacattg tttgcaacaa gccaggcttg aaaatactta taaatggtta
     1561 tagaaaatcc ttataaaaca ttaattcata gatacatta
//
LOCUS       DBBGLQ010000333         1565 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.339, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000333 DBBGLQ010000000
VERSION     DBBGLQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.339"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..178
                     /locus_tag="ACIYSB_005655"
     CDS             <1..178
                     /locus_tag="ACIYSB_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5432431.1"
                     /translation="KNALVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNK
                     DGDIQRRIMAMFSN"
     gene            complement(280..864)
                     /locus_tag="ACIYSB_005656"
     CDS             complement(280..864)
                     /locus_tag="ACIYSB_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
                     /protein_id="HGC5432432.1"
                     /translation="MCKCNTVTRDDIPFAIWSGLRPDTPHQCVRAQCGFRFSSASSPT
                     RSLTFGRWGCGERCHLSHVVGDSAVVLSGISRQIALQTRSEALRRTKRGLNARQGMEG
                     AKNAYRAFQEVRRADTPSQGLSGRQEPRQPPAKPICTVQAATRLVGGRRPSVLQRWGS
                     GVLPRRFPVALKALPEPWRLDRSGDRGAKTRKGF"
     gene            1264..>1565
                     /locus_tag="ACIYSB_005657"
     CDS             1264..>1565
                     /locus_tag="ACIYSB_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGC5432433.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERI
                     RELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLES"
BASE COUNT          406 a          383 c          413 g          363 t
ORIGIN      
        1 aaaaaacgcg ttagtcagaa ttcctgttgc tggtaaggtt tgtgcgtatg cactcgatcc
       61 acatgaagtc tggaagggat acaacacatc taaaaactac gcggcgtttg tcactaaaac
      121 actggtcaac aaagacggtg atattcagcg ccgaatcatg gccatgtttt caaattgagc
      181 cagcggcagg cacaggcaaa aaccccgttt ttggacaatc tgattcaaat gttaacattt
      241 gacatcgggt tgtctatcct gcattgctct tttgccgcct caaaatccct tgcgtgtttt
      301 tgctccccga tctccagaac gatcgagccg ccacggttcc ggcagcgcct tgagcgcaac
      361 cggaaaccgt ctggggagaa ccccagaccc ccatcgctgt aacaccgacg gtctacgacc
      421 acctaccaac cgagtggctg cctgtaccgt gcaaatgggc tttgcagggg gctggcgcgg
      481 ttcctggcga cctgaaagcc cctggctagg ggtatcggcg cgcctgacct cctgaaacgc
      541 tctgtaagcg tttttcgcgc cttccatgcc ctgacgggca tttaaacccc gtttcgtgcg
      601 tctgagagct tctgagcgcg tttgaagggc tatctgacga cttatccccg aaagaacaac
      661 agcggaatca ccgacaacat gcgagaggtg gcaccgctcg ccgcagcccc agcgaccgaa
      721 cgttagtgag cgagtgggcg aggaagcgga agagaaccgg aaaccacatt gagcacgtac
      781 gcactgatgc ggggtgtcgg ggcgaagccc tgaccagatg gcaaacggaa tatcgtcgcg
      841 ggtgacggta ttacatttgc acatcctgtc ccgatttctg aggcgtttta gcggtgaacg
      901 gacggcaaaa ccatgatgga gctgttgaac gttgagagcg tgaaatctgg caaaaatcac
      961 agctggtaag gcttgcagag gagtaagcca gtatacactc cgctatcgct acgtgactgg
     1021 gtcagggctt cgccccgaca ccccaaaaaa gtgttccgct ttgcgcaaca ctttcagcag
     1081 aaaagatatt taagggcgat tttgtaagtt attgattttt tagctacttg cattatgggt
     1141 tagctcacgc atgtgtggtc taaggttagc tcacgcatgt gtgagctagg tcgacctatc
     1201 atggtttacg tttgagctat gatggcttat atttgagcca tcattagacc gtggagattt
     1261 accatgtata tcgggactga agagcagcga aaaaagcgtc taaaagaact ggaaaaatta
     1321 aacaaagaat cggaatcaga tccgaaaaat aacggcccat tcacccaggt ttatcagaag
     1381 ggctgggaaa ggatccgaga actgtcgaag gacaagcagg gagttgtagc aataccgctt
     1441 tactcgtttt tagctgagca catagatccc tcatgcggtg ctgttgttgc cgatcagcag
     1501 tttttagcag ataaattggg tgttagccga agcacaatta agcgctggct caactactta
     1561 gaatc
//
LOCUS       DBBGLQ010000334         1545 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.340, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000334 DBBGLQ010000000
VERSION     DBBGLQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.340"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>1545
                     /locus_tag="ACIYSB_005658"
     CDS             <1..>1545
                     /locus_tag="ACIYSB_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5432434.1"
                     /translation="VNPPKAAAKALKQLGVETQDAYGNMRPMEDILYELYQATRKYGN
                     AAKVSFFSDIAGQEAYVSLMSLVEQAGEKNLPRLTREIQAANGELEENARIMADNLDG
                     DLKSLNSAWEGLRIRVADLVDGPLRSVTQSFTRVIAKVTALAQAHPELTKQLLIAGGA
                     LLAAVAATGALSLATGVLLGPLAKLRLGFSLLTGTSGLGRALPLLTQLRGVLGGSMSG
                     IGGWRVLFAGLDTGAGRLAVRLRGVRGLLGTLRGGLLAAFTSPGGALLSLGRGIGMLA
                     LRLSGLPALWGMVSGAVGALGGALGFLLSPVGLVVAALVGAAVLIWRHWDQLKAVVGG
                     YLSGLWQGLTPLREAVAPLGPIFEYVGQTIGRVWQWFVDLFAPVSTTKDELDKCTEAG
                     RTFGQKVGEFISNLVTGPVTLLVDSLTRVLEKLGLIPSAVERARKKAEELKQKELLNE
                     KAAMLLADVNVINPPQNDKDKKPPVPAPAATAPLTGENTGTMRRLNKIVDNTGGLLDE
                     AKQAKKR"
BASE COUNT          292 a          405 c          539 g          309 t
ORIGIN      
        1 gtgaacccgc ctaaagcggc agcaaaggcg ctgaaacagc tgggcgtgga aacgcaggat
       61 gcttacggga acatgcgccc gatggaagat atcctgtatg agctgtatca ggccacccgt
      121 aaatacggta acgcggcaaa agtctcgttc ttttcagata ttgccgggca ggaggcgtat
      181 gtctccctga tgagcctggt tgaacaggca ggcgaaaaaa acctgccgcg cctgacgcgt
      241 gagattcagg cagcgaacgg cgaactggaa gaaaacgcca gaatcatggc ggacaacctg
      301 gacggcgacc tgaaatcact gaacagcgca tgggaagggc tgcgtatccg tgtcgctgac
      361 ctcgttgacg ggccgctgcg ttctgtcacg cagtcgttca cccgggttat tgcgaaagtc
      421 accgcgctgg cccaggcgca ccctgaactg acaaaacaac tgctgattgc cggtggtgca
      481 ctgctggctg cggttgcggc cacgggggcg ctgtcactgg ctaccggcgt gttgctgggg
      541 ccgctggcaa aactgcgtct tggcttttcc ctgctgaccg gcacatccgg cctcggtcgt
      601 gcgctgccgt tactgacgca gctgcgcggc gtactcggtg ggtcgatgag tggtattggc
      661 ggctggcgtg tgctgtttgc cggtcttgat accggtgcgg gacggcttgc ggtccggctt
      721 cgtggcgtgc gcggactact cggtacgctg cgtggtggtc tgctggctgc gttcacttcc
      781 ccgggcggtg ctctgctttc tctggggcgc ggtatcggta tgctggcgct cagactgtca
      841 ggtctgcctg cgctgtgggg aatggtgagt ggtgcggtgg gcgcacttgg cggggcgctg
      901 ggttttctgt taagtccggt gggcctcgtg gtggcggcac tggtcggtgc ggcagtgctt
      961 atctggcggc actgggacca gctgaaggct gtcgtgggtg ggtatctgtc cggcctgtgg
     1021 caggggctta cgccactgcg cgaggctgtg gcaccgctgg gaccgatatt tgagtacgtc
     1081 gggcagacaa tcgggcgtgt gtggcagtgg ttcgtggacc tgttcgcgcc ggtaagcaca
     1141 acaaaggatg agctggacaa gtgcacggaa gccgggcgga cgttcgggca gaaggtcggg
     1201 gagtttatca gcaatctggt gaccggtccg gtcacgctgc tggttgactc gttaacccgg
     1261 gtgctggaaa aactcgggct gattccgtcg gcggttgagc gcgcccggaa aaaggcggaa
     1321 gagctgaagc agaaggagct gctgaacgag aaagccgcca tgctcctggc ggatgttaat
     1381 gtcattaatc cgccgcaaaa tgacaaagac aaaaagccgc cagtacctgc acctgcagca
     1441 actgctccgc tgacggggga gaataccggc accatgcgtc gcctgaataa aatcgtcgat
     1501 aacaccggtg gcctgctgga tgaggcgaaa caggcgaaaa aacgc
//
LOCUS       DBBGLQ010000335         1537 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.341, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000335 DBBGLQ010000000
VERSION     DBBGLQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.341"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..1178)
                     /locus_tag="ACIYSB_005659"
     CDS             complement(<1..1178)
                     /locus_tag="ACIYSB_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGC5432435.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGALIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     "
     gene            complement(1347..>1537)
                     /locus_tag="ACIYSB_005660"
     CDS             complement(1347..>1537)
                     /locus_tag="ACIYSB_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HGC5432436.1"
                     /translation="DTTGAGDAFVAGLLAGLADSGIPTNTRQLERIIAQAQICGALAT
                     TAKGAMTALPRQHDLPSQ"
BASE COUNT          377 a          376 c          348 g          436 t
ORIGIN      
        1 ctatcgcctt tgacgtaacg atctttactg ctttgtgcga gaattgctgg tgcgacatac
       61 caaccgcttc ccagaggtgt tgttccgtaa gatgcgaagc gccaggtatt cgcctcagac
      121 agcagggcgc catcggagcc aatccctttg acttccgcac ccaggccatg gccatatagc
      181 agtgctgttt ttgccgtccc ttcacgcagg ccatagaaag tgtcattgtg cagaccaact
      241 aacgcatgta cgccagtatt tgcagcatca gtttgaatga gagcaccatt ggcatctttt
      301 cgatcatcat tatcttttgc ccgtaatccg ctaaccatca actggaaggg gcctgcataa
      361 tgattcatgg tgaggatgta gttctgaacg ttattgtcga tatcatcaag atcgccgaaa
      421 ttacgtccgt agagagaaaa gttactgcgg aatgtatcgt tccattttac gtcatagata
      481 ccgccgcccg ttcccgctaa aaatacgaca tcagagtcta accagtgaat atcaaaattg
      541 tcgcgatcaa agcgtttacc agcccagaca gtactgtctt tgaatgctcc ggtaaaactt
      601 ggtaatgcgc ccagttcggc aaaagcctgt cggatattca ggttactgga gccgccagtc
      661 cagtcgttgt aatctctttg tccgtcagcc aacattgctt taaagcgtgt ggttgcaccg
      721 ttatccagtg tttgtttatg ttctacattt aactcgacat aggtatcggc ttcatttccc
      781 agacgaccaa cagctccacc agtttcacct gcgggagtca gatacggccc acttttgctg
      841 ctggaagcgg catcattcat cagtaacccg gaacgggcat agccatgaaa ctcaaaacca
      901 ctgttttctg cggatttctg ttcgagctga actgttctct gtgctacttc ctgagttgtt
      961 agctgatttt gttgttgtgt ttcagccagt ttctgaacct gtaattcagc tgttttggcc
     1021 cttgcttctg ccgcctgggc gcgggattcc gcatttttta aacgttgttc caacgccgcc
     1081 agtcgagatt cgatactgct aatatccgtt tgcgcatgta ccgttgtagc accggtcagc
     1141 aaagcaatta acactgccaa agttgttttt ttatacataa tttgtcgcgt cccatcagag
     1201 aatttaaaaa aaatgctaaa atgctaaacc ggtttaggcg ttgatgataa agctgacttt
     1261 ttatccagag caattatttt cgctaaaccg atttagtaaa tgtgacaggc acgacaaatc
     1321 gggttacaga tacaacagca ggatcactat tgtgaaggga gatcgtgttg tcggggtaag
     1381 gcggtcatcg cgcctttagc cgtggtcgcc agagcaccac aaatctgagc ttgtgcaatg
     1441 attcgttcaa gctgtctggt atttgttggt attccagaat cagccagacc tgcaagtaat
     1501 ccagcgacaa aagcatctcc tgccccggtc gtatcga
//
LOCUS       DBBGLQ010000336         1520 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.342, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000336 DBBGLQ010000000
VERSION     DBBGLQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.342"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            78..332
                     /gene="yjbL"
                     /locus_tag="ACIYSB_005661"
     CDS             78..332
                     /gene="yjbL"
                     /locus_tag="ACIYSB_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HGC5432437.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDVIIAKIDLFIQRITHNLWHARKKMCF"
     gene            356..1063
                     /gene="yjbM"
                     /locus_tag="ACIYSB_005662"
     CDS             356..1063
                     /gene="yjbM"
                     /locus_tag="ACIYSB_005662"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
                     /protein_id="HGC5432438.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            1426..>1520
                     /locus_tag="ACIYSB_005663"
     CDS             1426..>1520
                     /locus_tag="ACIYSB_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HGC5432439.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAP"
BASE COUNT          507 a          239 c          301 g          473 t
ORIGIN      
        1 tgagcgattt ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca
       61 aaaaaggtag atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa
      121 cactcaattc aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag
      181 gttcaatcaa aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc
      241 gtcatcggga tgtaattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt
      301 tatggcatgc acgtaaaaaa atgtgttttt aattgtgcaa ataaatgatc tcaaaatgtg
      361 ggtcaataag tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc
      421 atcagttgtt gaccgggcta tttttcattt cgcgattaat agtatatgta atcctgggga
      481 taataaagat gcaatgccca ttgaacaatg tacttttgat gtagaaacta agaatgacct
      541 tccctccacg gttcagctat tttatgagga atctaaggat aatgaacctt tagcgaatat
      601 acattttcaa gcaataggtt ctggtttttt aacgtttgtt aatgcctgcc aggaacatga
      661 tgacaacagc ttaaaattat ttgcttcgct gttaatttca ctttcatatt ctagtgccta
      721 cgcagattta tcagaaacag tgtatattaa tgaaaataat gagagctacc tgaaagctca
      781 gtttgaaaaa ttatctcaac gtgatatgaa gaagtacctg ggagagatga agcgtctggc
      841 tgatggggga gaaatgaatt ttgatggcta tctggataag atgtcacatc tggtgaatga
      901 aggaacgctc gatcctgata ttttaagcaa aatgcgagat gctgcaccac aattaattag
      961 cttcgcgaag tcgtttgacc caacctcaaa ggaagagatt aaaatactta cagacacttc
     1021 taaattaatt tatgatttgt tcggggttaa atcggagaaa taatatgtga agttcttcga
     1081 tagtatggaa ggcattatat aaaaggaccc aatatttatt gggttctttt ttctctatca
     1141 atgctattag cagggagata tatcaccaga gtttaatgtg tgatttttta tttatcgtcg
     1201 aacctggatt gtttatcatt gcccttaaca aagttaacgg ctaataagat tatttccatc
     1261 acttcgtgag agcttcatgc cttgagagga tctcaatttt cttttgcaat gagacaggcg
     1321 cttcctgttg ttatggtata gtaccccgct attgagcctc ctgaatagtg atgctgaata
     1381 acataacccc atgatatatc gataaaataa tctctacatt tgaaaatgca cggtaattct
     1441 gaaatgcaaa aaatcaacca aaccagcgca atgcctgaaa aaactgatgt tcactggagt
     1501 ggtcggttta gcgttgcacc
//
LOCUS       DBBGLQ010000337         1513 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.343, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000337 DBBGLQ010000000
VERSION     DBBGLQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.343"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>1513
                     /locus_tag="ACIYSB_005664"
     CDS             <1..>1513
                     /locus_tag="ACIYSB_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGC5432440.1"
                     /translation="PAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCD
                     QSVPDGFGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDK
                     AWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGQETATELVEDTQAIAR
                     YGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICD
                     DDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVNDG
                     VKVKVSRVPEGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIV
                     DNGAHFDGEQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFMLRLT
                     VAADDGSERLVSTARTTETTYRFRQLALGRYTLTVRAVNAWGQQGDPASVSFRIAAPA
                     APSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETTARYLGTG"
BASE COUNT          316 a          401 c          508 g          288 t
ORIGIN      
        1 ccagcataca gcaacaacat ggcctggtgt ctgtgggata tgctgaccca tccgcgctac
       61 ggcatgggga aacgtcttgg tgcggcggat gtggacaaat gggcgctgta tgtcatcggc
      121 cagtactgcg accagtcagt gccggacggc tttggcggca cggagccgcg catcacctgt
      181 aatgcgtacc tgaccacaca gcgcaaggcg tgggacgttc tcagtgattt ctgctcggcg
      241 atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga ccgaccgtcg
      301 gataaggcgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc
      361 cgctacagct tcagcgccct gaaagaccgc cataatgccg ttgaggtgaa ctggattgac
      421 ccgaacaacg gccaggagac ggcgacagag cttgttgaag atacgcaggc cattgcccgt
      481 tacggtcgta atgttacgaa gatggatgcc tttggctgta ccagccgggg gcaggcacac
      541 cgcgccgggc tgtggctgat taaaacggag ctgctggaga cgcagaccgt ggatttcagc
      601 gtcggcgcag aagggcttcg ccatgtaccg ggcgatgtta ttgaaatctg cgatgatgac
      661 tatgccggta tcagcaccgg tggtcgtgtg ctggcggtga acagccagac ccggacgctg
      721 acgctcgacc gtgaaatcac gctgccatcc tccggtacca cgctgataag cctggttgac
      781 ggaagtggca atccggtcag cgtggaggtt cagtccgtca acgacggcgt gaaggtaaaa
      841 gtgagccgtg ttcctgaggg tgttgctgaa tacagcgtat gggggctgaa gctgccgacg
      901 ctgcgccagc gcctgttccg ctgcgtgagt atccgtgaga acgacgacgg cacgtatgcc
      961 atcacagccg tgcagcatgt gccggaaaaa gaggccatcg tggataacgg ggcgcacttt
     1021 gacggcgaac agagtggcac ggtgaatggt gtcacgccgc cagcggtgca gcacctgacc
     1081 gcagaagtca ctgcagacag cggggagtat caggtactgg cccgctggga cacaccgaag
     1141 gtggtgaaag gcgtgagttt catgcttcgc ctgaccgtgg cagcggatga cggcagtgag
     1201 cggctggtca gcacggcccg gacgacggaa accacatacc gcttcaggca actggcgctg
     1261 gggcgttaca cgctgacggt ccgggcggta aatgcgtggg ggcagcaggg agacccggcg
     1321 tcggtatcgt tccggattgc cgcaccggca gcgccgtcgc ggattgagct gacgccgggc
     1381 tattttcaga taaccgccac gccgcatctt gcggtttatg atccgacggt acagtttgag
     1441 ttctggtttt cggaaaagcg gattgctgat atcaggcagg ttgaaaccac agcccgttat
     1501 cttggtaccg gaa
//
LOCUS       DBBGLQ010000338         1513 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.344, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000338 DBBGLQ010000000
VERSION     DBBGLQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.344"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..282
                     /locus_tag="ACIYSB_005665"
     CDS             <1..282
                     /locus_tag="ACIYSB_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGC5432441.1"
                     /translation="DNMEATGFYRVALTEAQPGDVLLCCFGASVPNHAAIYCGDGGLL
                     HHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            219..821
                     /locus_tag="ACIYSB_005666"
     CDS             219..821
                     /locus_tag="ACIYSB_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGC5432442.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDTGENELSARLNEPLANGAVIHIVPRLAGAKRGGIFQAVLGAALIATA
                     IWMPGLSIVASNLMFSLGAGMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVASQEISTADEGDGGQVVVIGR"
     gene            894..1232
                     /locus_tag="ACIYSB_005667"
     CDS             894..1232
                     /locus_tag="ACIYSB_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432443.1"
                     /translation="MNKTILFCTIIALTGCKSLDYVKSGKPVMEGNSLKNIDELSGCI
                     SRQWAGNGTPITSIPIENGVSLLVPQAMGGYDVVLDIKKAGNGSSFTLYERVPALTPK
                     IFADSVNACK"
     gene            1299..>1513
                     /locus_tag="ACIYSB_005668"
     CDS             1299..>1513
                     /locus_tag="ACIYSB_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432444.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIQGPVDGLKS
                     VLLNGTPVLDSEGNTNISGVTVVFRAG"
BASE COUNT          372 a          331 c          432 g          378 t
ORIGIN      
        1 gacaatatgg aggcgactgg tttttaccgt gtcgcactga cagaggcgca gcccggcgac
       61 gtgctgctgt gctgttttgg tgcatcggtg ccgaatcatg ccgccattta ctgcggcgac
      121 ggcgggctgc tgcaccatat tcctgaacaa ctgagcaaac gagagaggta taccgacaaa
      181 tggcagcgac gcacacactc cctctggcgt caccgggcat ggcgcgcatc tgcctttacg
      241 gggatttaca acgatttggc cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca
      301 tccgggcgct ggccacgcag ctccctgcgt ttcgtcagaa actgagcgac ggctggtatc
      361 aggtgcgtat tgccgggcgt gatacaggcg aaaacgaatt atcagcccgt cttaatgaac
      421 cgctggcaaa tggtgccgtg atccacatcg tgccgcgtct ggcgggagcc aaacgtggcg
      481 gtatttttca ggcagtgctg ggtgcggcgc taattgctac ggcaatctgg atgccggggc
      541 ttagtattgt tgcaagtaat ctcatgttct ctcttggggc aggtatggtt ttaggcggtg
      601 ttgcgcagat gctggcaccg aaagccagaa ctccccgtac acagacaacg gataacggca
      661 aacaaaacac ctatttctcc tcactggata acatggttgc ccagggcaat gtcctgcctg
      721 ttctgtacgg tgaaatgcgc gtggggtcac gtgtggcatc tcaggagatt agcacggcag
      781 atgaagggga tggtggtcag gttgtggtaa ttgggcggta atattattta ctcatgttct
      841 aactgattta atatttatat cgaacactga taattattct gttggttagc tatatgaaca
      901 aaacgatttt attctgcacg attattgcct taacaggatg taaatctttg gattacgtaa
      961 aatccggcaa gcctgtaatg gaaggtaatt cattaaaaaa tattgatgaa ttgtcaggct
     1021 gcatatccag acaatgggct ggtaatggaa cacctataac atccattcct attgagaatg
     1081 gggtaagcct tttagttcca caggctatgg gtgggtatga tgttgtgctt gatatcaaaa
     1141 aagcaggaaa tggcagtagt tttactcttt atgaacgcgt accagcatta acgccaaaaa
     1201 tttttgctga tagtgttaat gcatgtaaat aatagttaat cctgccgtaa ctcatgagcc
     1261 gccttttggg cggctttgtt gtttatggag tgtgaggaat gggtaaagga agcagcaagg
     1321 ggcatacccc gcgcgaagcg aaggacaacc tgaagtccac gcagttgctg agtgtgatcg
     1381 atgccatcag cgaagggccg atacaaggac cggtggatgg attaaaaagc gtgctgctga
     1441 acggtacgcc ggtgctggac agtgagggga ataccaacat ctccggtgtc acggtggtgt
     1501 tccgggccgg tga
//
LOCUS       DBBGLQ010000339         1506 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.345, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000339 DBBGLQ010000000
VERSION     DBBGLQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.345"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            147..472
                     /locus_tag="ACIYSB_005669"
                     /pseudo
     CDS             147..472
                     /locus_tag="ACIYSB_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            536..1099
                     /locus_tag="ACIYSB_005670"
                     /pseudo
     CDS             536..1099
                     /locus_tag="ACIYSB_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
BASE COUNT          412 a          329 c          338 g          427 t
ORIGIN      
        1 taactgaatt atgggggctt atgtccgaac tatcgtaagt tatcgcgtgg atttgtcgtt
       61 aatgcgtagc tttgtcctca gcgcagggga ctatgacact tcatcttctg tggtggtacc
      121 ccgtggggca ggtacagata acgcatatag ttcataaatc tgacagtgat gaattagctg
      181 cactaagggc aagcataact tgaagctcat gggattgaat ggtgacgtaa accgcagagc
      241 cctgtgcagt gcgttttcgt attatccacc gacgagaagg ttgcattatt tcgtcggttg
      301 tttcgcgggc gtgatgatgt atgggccctt cgatgggaaa gcaaaaccag cggaaagggt
      361 attcgtctac ctggctaacg aatggctgtt gggaatatgc agtaagccct ggataaaatg
      421 tggggactgc gttcaccgtc agttgatccc tgtatctgat ctcatcatct acctgaggta
      481 gtgtacaata tgccgatatc gaactgatac ttaattaaaa tatctagagt gtgatatatc
      541 tctgagaact atcaatacga atattaaaaa tacttccaga ataacagcaa tacgggtgga
      601 tctccattac cctcctgtga ttgacaacgg ggataccatt acctgtttcc ctaacctgat
      661 ccccggagag atatctcgtt tccgtaactc gctgaatgcg aaaattaatg cagatcaaca
      721 ccgtaaaaaa caataaggca aacgcaccta cgcaacgtcg gtttgtatta tctgggcgaa
      781 agaatataca cagtccttta aatgtcatta tcacgtctgc ctgttgttta acaaagacgc
      841 ctattaccac ctgggagact atgagcgctc tgacaccctg agagggttaa ttaccggagc
      901 ctggtacagc gccttagggt tggaactgga agatcaccag ggattagttc atttccctac
      961 taatggacgt tacatcctgg atattaacga tcctgatttc cctgagcagt atcagcgctt
     1021 attggcacgt ctggattatc tgacgaagct cgacactaaa gcatcaggtc agcgtaactt
     1081 tggctacagc ggtttttgac ctattgtaac tgtaaggtga tttattatgt atacagaatc
     1141 aaacattaag ctttcctgta acgtcctctg gcaaaagtgt gtccttgcaa ctctgtatcg
     1201 ggctgcagct acacatccta aaattaaatt attacaattt aaattaatac cttgcccagg
     1261 tatgaataag gcaattccct ctctgatggc tgattttact ggtgaactgc atagactaat
     1321 ttctgaatat ttgcagtgca agcgacgtaa agataaatct gttgagcccg cccctttata
     1381 ttatctctgg agtagggagg ccatggctaa cgattggatt tgcccctata tttccagaca
     1441 cctgttatca cttaacccat tactggcccg ctgccgtaga tattcccgtg gcgagcgata
     1501 acccag
//
LOCUS       DBBGLQ010000340         1502 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.346, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000340 DBBGLQ010000000
VERSION     DBBGLQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.346"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..205)
                     /locus_tag="ACIYSB_005671"
     CDS             complement(<1..205)
                     /locus_tag="ACIYSB_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B562"
                     /protein_id="HGC5432445.1"
                     /translation="MKKQKVFFSGAVLAIFLSLSNVSLASGLDKNMQSLAKSMSILQS
                     SNDKSSLLNALEMMDKSVDSSMNI"
     gene            complement(639..1022)
                     /locus_tag="ACIYSB_005672"
                     /pseudo
     CDS             complement(639..1022)
                     /locus_tag="ACIYSB_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(1160..>1502)
                     /locus_tag="ACIYSB_005673"
     CDS             complement(1160..>1502)
                     /locus_tag="ACIYSB_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432446.1"
                     /translation="PHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGTSATPVSEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISSLQNAGVIIANSNAQAALWASTVA
                     QTQLQKKELNA"
BASE COUNT          404 a          307 c          336 g          455 t
ORIGIN      
        1 taatattcat agaagaatct acagatttat ccatcatttc aagagcattt aatagtgatg
       61 atttatcatt ggaagattgt agaatactca tacttttggc aagagactgc atgtttttat
      121 ccaggccgga agccaaagat acgttgctga gagataaaaa aatagctagt acagcacctg
      181 agaaaaatac cttttgtttt ttcattaata tattcctgtt caaagttgat tatagttatg
      241 tcaatttaag tttattatta tgatttttta tttttgtact atgaatctcc tgtatgctta
      301 tatgtgcaga aaatctcatt ttatcaagaa gaatatcaaa ttttctccga tacttctaca
      361 tactccatca ttcatgctcc ttttatccgt gcaccgtaaa accccgtcct tcagcgggga
      421 tataaggcgc tgttttccac ctaaccaggt gtctgctgct attcaatgta ttggcggatg
      481 acggatgccg cccgttgaag caggaaccct cgtccttagg gcgtggagga tgtcaaaatg
      541 aaaacttgtg gtatgaatca atccattcat catcctttcg gatcattgca ttcagtataa
      601 cgctggaaga gcctggcaat atcactgtat tgatggtatt acgccatgaa ttgcttattt
      661 cttaaatacg acatgcacca cagacaggct tttcctatac catcggaacg agcggcaaga
      721 aataacttgg tggcgtgttt atgttcgcgc attggctttt taagcaattt acctctgttc
      781 aataacggca atgccagatg atgtggaata taaccaatac cgaagttttg ttctattaag
      841 ctgattgcca tggcgaagtc aggaacaaaa ataggttttt gtccttccaa caaccaggct
      901 tgcattgggg ggaagttaac ggaggtatcc cggatacaaa ccgcaggata atgacgcaat
      961 tcactgtttt ctaatggatt ccgcttcact gccagtggat ggcgtagtaa tgacgccaga
     1021 accaaaccgg tgtcaatatc accttgtgct ggcggctgcc agttgaggga tatagcatgc
     1081 cctcctgcat gttgaatatt gtcagcaaaa ctgtgtagcc ccgcgttcac gaccttcaaa
     1141 ggctgattaa ataaggtatt catgcattta gctccttttt ctgcaactga gtctgtgcaa
     1201 cggtgctggc ccatagcgcc gcctgtgcat tgctgttggc aatgatgacg cctgcatttt
     1261 gtagtgagct tatctgctga ctgcgtattt gcggatcagc ttctgttccg caaacgtggg
     1321 caatcagcaa cggtgcatta ttcatgtcga tgtgtgaaag ttggtccagt aattcgctga
     1381 caggcgtagc ggaggtgcca taaccaagta caaggtcgaa taacacaacg gcagtatgtg
     1441 agtcgttgag ttcgttgagc agacgttgat tgcgcagagt cgggtcgatc attggatgcg
     1501 gt
//
LOCUS       DBBGLQ010000341         1490 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.347, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000341 DBBGLQ010000000
VERSION     DBBGLQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.347"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(165..1151)
                     /gene="ycdU"
                     /locus_tag="ACIYSB_005674"
     CDS             complement(165..1151)
                     /gene="ycdU"
                     /locus_tag="ACIYSB_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGC5432447.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(1148..>1490)
                     /locus_tag="ACIYSB_005675"
     CDS             complement(1148..>1490)
                     /locus_tag="ACIYSB_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGC5432448.1"
                     /translation="AWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVAPSI
                     IKENLSLHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRVMHC
                     RKAFSDAKVFK"
BASE COUNT          502 a          349 c          277 g          362 t
ORIGIN      
        1 cgggcttttt tttcgtatat tgcacacacc gggtgccccc gcccacgtct gttagggcga
       61 ggggaaatcg tgcacatcaa ctgtctggtt actcagtcaa caatcaaaat gcgcaagaga
      121 gtacagaaca tatatgtatt atctggtcac ttcctatcac ttcctcactt attaaaatgc
      181 ccccaacgga aatacatgtc acatgccatc ataaaatcgt tatgccagag gaatcgacaa
      241 tagaaaaaat agtaaattaa gcacggtaca tagcagagta aaaaataggc ttcaaaccaa
      301 atccgccatt ctacaagcgt tgccaatacc gacaccagcg taatccacat cacttcaata
      361 accagcaata tgaataaccg aagccaaagc ggcttggcaa aaaaggtcga caacgcattt
      421 tcactggaga aaaacagatc gatttcctgc aaaaaaagcc tgcccagaaa agcgtaccgc
      481 ttggagaagc gcagaaaaaa cagattaatc gccaacacta cgccacaaag aatgagcaat
      541 gcaattatcg atttaaacgt taaccactgc ccctctgtca caaaagaatc actgttacgc
      601 aacgggctga tagtaagcag gttaacaacc gttgagatgc acaccgaaag cagcacatag
      661 cgagtcagac ttttatattt tacttttttc gctactgaaa tagcgacctg cttgacataa
      721 ctggtgcgga tcatccgaaa cagcagaaag atgaccaacg gcccaagtaa cgaaatcagg
      781 aacagcgcat ataacgaggg tagaagctgg tatagccccc aactcattgc actcaacatg
      841 attaacgaca gaaatcctgc caccagaggt tgtgtaagaa ataattctat tttgtggttc
      901 tgctttttct taaaccacga aaaatcgata ttcttcgtga cgcatttata agcccagtac
      961 gccttgagat caaaaataaa attatacagc accagagcca gaatcccact aactacacca
     1021 atatcctctg atggaaaagt aaaccctgca ttcttccaat aaaagcctat cagaataagt
     1081 ccaatagcat aaatgtatat cagatttcgt ttatcctgaa agcgaataaa agttaaataa
     1141 tcaggaatca tttgaatacc tttgcatcac taaatgcttt acgacaatgc ataactcgtt
     1201 gctgatccac gccagcaaaa tcatttaaca tattccccaa atagggctta ttacttattt
     1261 caaggaaagc taccggggtt ggcatattca gaaatgtatc gagagattca gtccatttca
     1321 ccgtatgagt taagtgtaat gagagatttt ctttgataat tgacggagcg acctcgcttt
     1381 ttgctgttac gttcatcaga acctgatgct ctggcgaagc gatatccagt ccggcaagat
     1441 aatcgcgcat tgcctgaacg ccgtcttcca tcaaacgcgt gtgccaggca
//
LOCUS       DBBGLQ010000342         1459 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.348, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000342 DBBGLQ010000000
VERSION     DBBGLQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.348"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(98..871)
                     /locus_tag="ACIYSB_005676"
     CDS             complement(98..871)
                     /locus_tag="ACIYSB_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HGC5432449.1"
                     /translation="MIKHTLLMPFFFSALPAYAGLTSITTGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAAGSYSNMISVRLKDPRGAGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYKTPNHNFNGLQLGLHLKWQL"
     gene            complement(873..>1459)
                     /locus_tag="ACIYSB_005677"
     CDS             complement(873..>1459)
                     /locus_tag="ACIYSB_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGC5432450.1"
                     /translation="KLKNYLELNVKEKNTQIADQVIIDESAGEVVIGANTRICHGAVI
                     QGPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIETEATIGPQCFVADSVV
                     ANQAYLGAQVRTSNHRLDEQPVSVRTPDGIIATGCDKLGCYIGQRSRLGVQVIILPGR
                     IISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
BASE COUNT          393 a          342 c          323 g          401 t
ORIGIN      
        1 tccctgaaaa tatcatatat gaacttataa tatcattcga tagatgataa gtcatcaccg
       61 gccttaaata attatgtgaa ccatgttcat ggttgtatca taattgccat tttaaatgca
      121 ggcctaattg cagcccattg aaattatggt tcggtgtttt ataccaaact ttacctgccg
      181 tcagcccgag attaagttgc tcagtcagcg gttgaatacg ttgtaaactg acgctcttca
      241 tgctgccgta tcgtgtttct ggcgtccagt cataggtgta agcacctgtt ccgcggctta
      301 gccatagttg tgtataacca gcaccgcgcg ggtccttcag acgcacggaa atcatattgc
      361 tgtaactacc agctgcatcg ctggagtcat aatgggtata gcggtagctg gtgatcaccg
      421 ggccagtata gagagatacg ccgccttgcc aggcatcgac ttcgacatca tcgtaatatt
      481 tagtgtagcg ataaccggtc gtgaaaagcg tttttggcaa cagggccagg ttaatatcct
      541 gacgaaaatc ctgacgggca aagaccggcg tattatccgc aaaggcaata cccgttcggg
      601 ttgtcagcca gttattccat ttgtaccaga ctgcaccctg gccgcgagtg gcattgaaat
      661 gttcagtttc ataatcacga cgcccctggg aaagattaaa aagcagcgtt gcgttttcaa
      721 cttttgccac cagttcagca taagctaaat tacggttgcc atggtcgcca gaataatcgg
      781 taaaatcgta gcccgtggta atggaagtta acccggcata agcgggcaat gcactaaaaa
      841 aaaacggcat taataacgta tgctttatca ttttaatctc ctgtacggat aagttcttgt
      901 cggagtgagt aagttccggt aggtaaatta cgttctacaa tcacgcgcgg gccaagttgc
      961 gtgttcgggg aaataattcg cccgggcaaa ataataacct gtacgccaag gcgtgaacgc
     1021 tgcccgatat aacaacctaa tttatcgcat ccggtagcga taattccatc tggcgttcga
     1081 acagacacgg gctgttcatc cagacgatga ttactggtac gtacttgtgc gcccagatat
     1141 gcctggtttg caactaccga gtcggcaaca aagcattgcg gaccaatcgt tgcttccgtt
     1201 tcaataaccg catttttaat ttcggtggca aaaccaattt ttacgccatt gctgattatt
     1261 gtgccaggac gaataaacgc ataattacct atcaggcagt ttgcgccaat cactaccggc
     1321 ccctgaataa cggcaccgtg gcaaatacgg gtattcgcgc cgataacaac ttcacctgca
     1381 ctttcatcaa taattacctg gtctgcaatc tgagtatttt tctctttaac attaagctcc
     1441 agatagtttt tcagtttct
//
LOCUS       DBBGLQ010000343         1458 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.349, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000343 DBBGLQ010000000
VERSION     DBBGLQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.349"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..1458
                     /locus_tag="ACIYSB_005678"
                     /pseudo
     CDS             1..1458
                     /locus_tag="ACIYSB_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558154.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          442 a          296 c          358 g          362 t
ORIGIN      
        1 gtccatacgg catattgctc gtaaactgga cctgagccct tcagtaatag caagatggag
       61 acttgaactg gtgcaacaag gcctgttacc tgaaaatgaa aaaatatctt ctgtcgatgg
      121 aaatgaatgg acaccggagc gacgattttc tgttgtaatt gaaactgcaa cattatctga
      181 aatggaactg gctgattatt gtcggagaat ggggttattt gttgagcaag ttaaagaatg
      241 gagagctata tccattcagg ctcatgaaat gaaaatggca gaaagtcgcc gtgctgataa
      301 agaattacgc gaagcaaaga cccagttacg ggaactggaa aaagaacttc tccgtaaaga
      361 taaagctctg gcggaagcag ctgctctttt gatgttaagg gaaaagttca atgccctctg
      421 ggacaacagc gaggaaggct gactcctctt ccagagcggc agaagattat taatctggtc
      481 agcgaggctg tcagttcagg agcgagaaaa acacaagcct gccagtgtat tggtatttca
      541 gtcagaacac ttcaacgttg gatgcagggt ggtgtggttc atacagatca gagaccaggt
      601 gcatcaaggc gtaccccggg gaataaactg tcagaaacag aaagattaag aatacttgat
      661 gtatgcaatt ctccggagtt tgcatcattg tctccgaata ttattgttcc gactcttgcc
      721 gacagagggg tttatatcgc gtcagaatca actttttatc gcgtattaaa atcagctaac
      781 caactgacaa ggagaacccg tgaacaccaa tataagcggc ctgaagtact caaagctgta
      841 gcccccaacc aggtgtggag ctgggatatt agttatatcc catcgtgggt taaggggcag
      901 catttttatc tctatatgat cgtcgatatt tatagccgaa aaattattgc tgccgaagta
      961 tttacgaaag aatctgggca gtatgcggca gaactgcttc aacgtgctgt atggaatgaa
     1021 aaatgttcat cccgcaatct cgtgcttcat tctgataacg gaggaccaat gcgcagttgt
     1081 acgttactgg caaaaatgta cgcgcttggc gtattaagtt cttattcccg tccccgggtc
     1141 agcaatgata acccttattc cgaatcgctc ttccggacga ttaagtattg tccatggtgg
     1201 ccagagaatg gttttcgaac catagatgaa gccagaagtt ggttaagcaa gttcacgcga
     1261 tggtacaacc tggaacacaa gcacagcggt attaagtatg ttactccgga tgaacgccat
     1321 cgcggaattg acgcacaaat actggaagcc agaaaaaaag tttatcgcga agctcgcaaa
     1381 cgtcatcctg agcgctggag caaacagttg cgtgactggg aactcattca ggcagtttat
     1441 ctgaatccag aaaaagaa
//
LOCUS       DBBGLQ010000344         1431 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.350, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000344 DBBGLQ010000000
VERSION     DBBGLQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.350"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..339
                     /locus_tag="ACIYSB_005679"
     CDS             <1..339
                     /locus_tag="ACIYSB_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGC5432451.1"
                     /translation="TALNAENVRPVADRLTVQSATIVNWEMEAKLYFYPGPESEPMLA
                     AADAAFRAWLADQGRIGQDVALSAITAALHVHGVQRVEVIKPTQNITISDTQAARCTT
                     FSISEGGRNE"
     gene            332..895
                     /locus_tag="ACIYSB_005680"
     CDS             332..895
                     /locus_tag="ACIYSB_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGC5432452.1"
                     /translation="MSDRKKTDSAQHSTSLLPPSAGPFMRATEATGARISAIPVDADT
                     LWSPDRCPAHLLPYLAWACSVDSWDRNWPEETRRQVIRDAWMIHRHKGTISALRRIVE
                     PLGYLIRVSEWWEFDGSPGTFTIEIGTLETGVSEEVHEEMERLIADARPVSRHLVGLS
                     IIQEIHGAIYAAAAGYDGDIITIYPED"
     gene            899..>1431
                     /locus_tag="ACIYSB_005681"
     CDS             899..>1431
                     /locus_tag="ACIYSB_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432453.1"
                     /translation="MSTRKFRTLITDIGLAKLAATTAPGGKPVRLTHMAVGDGNGELR
                     QPQKNQTSLYNEVWRQSVNRVFTDPENPNRLIAELVIPPETGGFWVREIGVFDDTGTM
                     IAVGNTAESYKPTREEGSGRAQIFRAVITVTSDAVVELVMDTTTILPTTDYIDEKIAE
                     HARSRNHPDATLTEKGFT"
BASE COUNT          357 a          419 c          396 g          259 t
ORIGIN      
        1 accgccctga atgccgagaa cgtgcgcccg gttgccgacc gcctgactgt gcagagtgct
       61 accatcgtga actgggaaat ggaagcgaag ctgtattttt atccagggcc ggagtctgaa
      121 cccatgcttg cggccgctga cgccgctttc cgtgcatggc tggcagatca gggccgtatc
      181 ggtcaggacg tggccctgtc agccatcact gcagcgctgc atgttcacgg tgtccagcgc
      241 gttgaggtca taaaacccac gcagaacatc accatcagtg acactcaggc agccagatgt
      301 accacattca gcatcagcga aggaggacgc aatgagtgac aggaaaaaaa cagacagcgc
      361 acagcacagt acatcactgc ttccaccgtc cgcagggcca ttcatgcgcg ccacagaggc
      421 gacaggagca cgcatcagtg ccataccggt ggacgccgac accctgtggt cgccggaccg
      481 ctgccctgct cacctcctgc cctaccttgc atgggcatgt tccgttgaca gctgggaccg
      541 caactggccg gaagagacca ggcgacaggt gattcgggac gcatggatga tacatcgcca
      601 caaagggacc atcagtgcac tccgccgcat cgttgaacca ctgggctacc tcatccgtgt
      661 atcagaatgg tgggagtttg acggctcgcc cgggaccttc accattgaga ttggcacact
      721 tgaaaccgga gtaagtgaag aggtgcatga agaaatggag cgtctgattg ccgatgcgcg
      781 ccccgtcagc cgccacctcg tggggcttag tatcatccag gaaatacacg gcgccattta
      841 cgccgctgcc gcaggttacg acggcgacat catcaccatt tacccggaag actgaaccat
      901 gagcacgaga aaattcagaa cccttatcac cgacatcggg ctggcaaagc tggccgcaac
      961 aacggcgcct ggcggtaaac ctgtacgcct tacgcacatg gctgtagggg acggcaatgg
     1021 agagcttcgt cagccgcaga aaaaccagac atcgctttac aatgaggttt ggcgccagtc
     1081 tgtcaaccgc gtcttcaccg acccggagaa cccgaaccgc ctcatcgccg aactggtcat
     1141 tcctcctgaa accggcggat tctgggtgcg tgaaattggt gtgtttgatg atacaggtac
     1201 aatgattgca gtaggaaata ccgctgaaag ttacaagccc accagagagg aaggttcagg
     1261 gcgggcgcag atattccgtg cagtaatcac cgtcacctcc gacgccgtgg tggaactggt
     1321 catggacaca acaacaattc tgccgaccac ggactatatt gatgagaaaa tagcggaaca
     1381 cgcacgttcc cgtaaccacc ctgacgcgac actgacagaa aaaggattca c
//
LOCUS       DBBGLQ010000345         1429 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.351, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000345 DBBGLQ010000000
VERSION     DBBGLQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.351"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(301..963)
                     /locus_tag="ACIYSB_005682"
     CDS             complement(301..963)
                     /locus_tag="ACIYSB_005682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432454.1"
                     /translation="MFALKLTDERGREWISPVAPPLNLVRRFHFTLSARDAVMQFIDT
                     GVPTNYRCVIFIKQISGNQSFHGEMVQLNGTWQYDIFSAGTGQEPGMEQDSTYVVYVF
                     ANMVNVWPQWGIQLCDSHGRVVWNTEMLPLEVKMTRIWEKDVPISVGHDVAVMPGCTQ
                     WTTVQEGESYYWYTASFNARNSQLWRQSTFAEDNVAAPNDNSGVVNNLCYYINAEIYD
                     AY"
     gene            complement(963..1187)
                     /locus_tag="ACIYSB_005683"
     CDS             complement(963..1187)
                     /locus_tag="ACIYSB_005683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432455.1"
                     /translation="MTHEINPTGVLDVITEGTGQRSYTPLQGWRLEVCSPAILINGQG
                     SHTSASVSGWTVHYANTSWLRPVLVMIRGI"
     gene            complement(1223..>1429)
                     /locus_tag="ACIYSB_005684"
     CDS             complement(1223..>1429)
                     /locus_tag="ACIYSB_005684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432456.1"
                     /translation="KLTVDFARVTGDIRSDNFHSGSPGWRLSRNGSLEINSGRPGAGR
                     LFFNGERIDVYDDNNVLRVRLGRL"
BASE COUNT          368 a          415 c          273 g          373 t
ORIGIN      
        1 tcgtcattca tttacaatcg ggtgttttta tgattacttc acgcacgaaa ctttcgtaaa
       61 catatcttca ctcacccact gaccataaaa tctcggagct gatactttat acacagtgcc
      121 gcttttcgtt tctattttcc ctgtaagatc taccgtataa gctcgctgta aaaccccggg
      181 actcagcaca ccggtgcacc ggactctctc cgtaacggaa cggtaccccg cgcaacctga
      241 tacaagaaac agcaaaaaag tgcagacgat tattttcatt acatgccctc aacataacta
      301 tcagtacgca tcataaattt ctgcatttat gtaataacag aggttattaa caacacctga
      361 attatcattg ggcgccgcta cgttatcttc agcaaaagtt gactgacgcc ataactgtga
      421 gtttctggca ttaaaactgg cggtatacca gtaataactc tccccttcct gaactgtcgt
      481 ccactgggta caccccggca ttacggccac atcatgtccc acactgatcg gtacatcctt
      541 ttcccatatt cgcgtcattt ttacttccag tggcagcatt tccgtgttcc agaccaccct
      601 gccatgactg tcgcacaact gtattcccca ttgcggccat acattcacca tgttggcaaa
      661 aacataaaca acatatgtgc tgtcctgttc catgcccggc tcctgccctg taccagcgct
      721 gaaaatgtca tactgccagg taccgttaag ctgaaccatt tcaccatgga aggactgatt
      781 accgcttatc tgtttaataa aaatcacgca acgataattc gtcggcaccc cggtgtcgat
      841 aaactgcatc accgcatcac gcgcactcag tgtgaaatga aaacggcgca caaggttcag
      901 tggcggcgca acaggtgata tccattctct cccccgctca tcagttaatt taagtgcaaa
      961 catcagatcc cccttatcat gaccagcacg ggtcttagcc agcttgtgtt ggcatagtgc
     1021 actgtccagc cggatacact ggcactggta tgacttccct gaccgttaat cagaatggcc
     1081 ggtgaacaga cttccagacg ccagccctgc aaaggagtat aagaacgctg ccccgtccct
     1141 tcagtgatca catccagcac ccctgttggg tttatttcat gtgtcacaag gcgccctttg
     1201 cgccataccc ggactccgaa cattacagtc tccccagtcg tactcttaac acattgttat
     1261 cgtcatatac gtcgattcgc tcgccattga aaaacaggcg gcctgcgccg ggccttcctg
     1321 agttaatttc aaggctgccg tttcttgata aacgccaccc cggactaccg gaatgaaaat
     1381 tatctgaccg gatatctccc gtcactctgg caaaatccac ggtcagttt
//
LOCUS       DBBGLQ010000346         1408 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.352, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000346 DBBGLQ010000000
VERSION     DBBGLQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.352"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..356)
                     /locus_tag="ACIYSB_005685"
     CDS             complement(<1..356)
                     /locus_tag="ACIYSB_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432457.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFTSYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTL"
     gene            complement(353..859)
                     /locus_tag="ACIYSB_005686"
     CDS             complement(353..859)
                     /locus_tag="ACIYSB_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432458.1"
                     /translation="MATSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(834..1244)
                     /locus_tag="ACIYSB_005687"
     CDS             complement(834..1244)
                     /locus_tag="ACIYSB_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGC5432459.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VAFRAWAKVVQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGEFTQSHGGNNGDFLFAR"
     gene            complement(1241..>1408)
                     /locus_tag="ACIYSB_005688"
     CDS             complement(1241..>1408)
                     /locus_tag="ACIYSB_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGC5432460.1"
                     /translation="DSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFS
                     WLVGPYRYFPQ"
BASE COUNT          309 a          434 c          370 g          295 t
ORIGIN      
        1 agcgtgccgc cgtcataggt aatcgggtta ccgcacgctt ccgggttcca gcccagcagg
       61 gccttaaaga gcatctgccg gacatcgtcc accacatcat acgaggtaaa ctgaccgcgc
      121 tcatcacgcc cgttactcag tatgacaacc acggagaaac cctctttcag ctcctgccag
      181 tagtcggtct ggcttttgtt ttctcccgga gaatcatcac ccggtacaac atatgccgcc
      241 gggagtttca gctttccgac ctccggcaga tttttgaact gggccgcgcc tgcaacccgg
      301 ttttcaaaat acggacagcg ggcacgcagt gcagcaataa caggcgtcag tttcatctgt
      361 gtcgtcgctc cggcttcagt gatttacgca attcccgcgc cagaaaatag cgtgtccagc
      421 tgcggttctt ttcaagcgtt tccaccatga agttattacg tggagccagt cgccagccgc
      481 tgccaccgga tgcaccacga tgatggctgc gacgacgctt tgcccctcgc ctcacgccat
      541 agaacaaaaa agccggataa aaatcaccgg tgatacggcg gtttccctct ccattacgct
      601 ggttaggggc tatacgtgcc ataaaaccag gacgatgttt actggctctg ggtaccatgt
      661 aaccaatcga acgagccagg cgtccggtct gataaccggg gttttcaccc ggtgccgacc
      721 gcgcacggcg catcaccagc cgacgggcat cacgcatatg acgctgacca atcgtgacaa
      781 acgcccgccg gacacgggcg cggttaaagc gcatctccgc gggctgctga aaatcaacgt
      841 gcaaaaagga agtcgccatt gttgcctccg tgactctgcg taaattcgcc cagctccgta
      901 cactccagca gcagaaagcg ccgcgccccg ttcagatcac gctgacgttt cacccggtac
      961 acactgtcac cgcagaccac ctcataatca gcggtgatcc cccggcggta gcgaatggtg
     1021 atgtaatggg tgatggcgtc tccggtctgc gcggtttcct gccaggtagt ggcactggtc
     1081 tggacaacct tcgcccatgc ccggaacgca accggatatt gaggctccac gccaaagtta
     1141 tccgcgggca tatccacccg ctggcggatc aggacgcgtt tattcagttc gccggggtcc
     1201 ggcagaatgt aggttgcgct ggtctgcgcc tgacgaattt tcattgcgga aagtacctgt
     1261 acgggccgac aagccagcta aaactctgcg gcatgtcgag tttctccact tccgtaaccg
     1321 aagagcggtt ttcgtaaaaa tggctgataa gcatcagcat ccccagacga atatcatccg
     1381 gcaggtgcag cccgtccgga tcgctgtc
//
LOCUS       DBBGLQ010000347         1400 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.353, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000347 DBBGLQ010000000
VERSION     DBBGLQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.353"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..288
                     /locus_tag="ACIYSB_005689"
     CDS             <1..288
                     /locus_tag="ACIYSB_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432461.1"
                     /translation="RGWDDGRGVDAGRGILSIQGWLTGSHYHNIRSWDAWDNTVLVPN
                     DRGGDSLLSTDNAVREGAINGKFSSQYRTESSGGNETRPRNIAFNYIVRAA"
     gene            288..899
                     /locus_tag="ACIYSB_005690"
     CDS             288..899
                     /locus_tag="ACIYSB_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGC5432462.1"
                     /translation="MNNAILNNDLIAVQAGNIIVYNYDGETREYISESTEYLAVGVGI
                     PANSCLDAPGAHKSGYAIRRSEDLNSWEYAPDHRGETVYSTDTGNTEEITALGDYPKN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHRAAVEAAETKRQSLANAAMDSISLIQLKLRAG
                     RKLTQAETTRLNAVLDYIDALNAMDINMAPDINLPEIPLASAS"
     gene            complement(906..>1400)
                     /locus_tag="ACIYSB_005691"
     CDS             complement(906..>1400)
                     /locus_tag="ACIYSB_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432463.1"
                     /translation="GEGSALPVGVPIPWPSATPPTGWLKCNGAAFSSEKYPNLARVYP
                     TLKLPDLRGEFIRGWDDGRGIDSGRSILSEQGYATEDHAHGLPSKSTVATDRSINFYF
                     DEAWATSGNTGVIRWGNTSDAGLPAPNYGTFKTYKQSVANLGTAGLETRPRNIAFNYI
                     VRAA"
BASE COUNT          388 a          318 c          361 g          333 t
ORIGIN      
        1 cgcggctggg atgatgggcg aggcgtggat gcgggaaggg gcatcttaag catacagggg
       61 tggttaactg gaagtcatta tcataatatt cggtcatggg acgcgtggga taacacggta
      121 ttggtgccaa atgacagagg gggggatagt ctgttgtcga cagataacgc cgtccgcgaa
      181 ggggcgatta atggtaaatt ttccagtcaa tacagaacgg agtcctcagg ggggaatgaa
      241 acccgtccac gcaatattgc cttcaattat attgtgagag ccgcataatg aataacgcga
      301 tattaaataa tgatcttatt gccgtacagg caggaaacat catcgtttat aactatgatg
      361 gtgagacacg ggaatatatt tcagagtcaa ctgaatatct tgctgtgggt gttggtattc
      421 cggctaattc ctgtttagat gccccaggcg cacataaatc tggctatgct attcgccgtt
      481 cggaggattt aaattcatgg gagtatgcgc cagaccatcg tggtgaaacc gtctatagca
      541 ctgacacggg aaacaccgaa gaaatcacgg cgctgggtga ctacccgaaa aatacaacca
      601 ctatcgcccc gttaacacca tacgataaat gggatggtga aaaatgggtg acagatactg
      661 aggcacaaca tcgtgccgca gtagaagcgg cagaaaccaa gcgccagtca ctggctaatg
      721 cggcaatgga ttccataagt ctgattcagt tgaagttgcg ggccgggcgg aagttgacgc
      781 aggcagaaac cacccggctt aatgccgtgc tggattatat agacgcgctg aacgcaatgg
      841 atataaacat ggcaccagat atcaacttgc cggaaatacc actggcatca gccagttgaa
      901 tataactaag ccgccctcac gatatagtta aatgcaatat tgcgggggcg ggtttctaag
      961 ccagcagtac ctaaattagc tacggactgt ttatatgttt taaaagttcc ataattaggg
     1021 gctggcaatc ctgcatcgct tgtgttcccc catctgataa ctcccgtatt accactagta
     1081 gcccatgcct cgtcaaagta gaaattaatt gagcggtcag ttgctacggt tgattttgac
     1141 ggtaatccgt gagcatgatc ctccgttgca tatccttgct cgcttaaaat agagcgacca
     1201 gagtcaatcc ctcgcccatc atcccagcca cggataaact caccgcgtaa atcaggtaat
     1261 tttaacgttg ggtaaaccct tgccagattt gggtactttt cagaagaaaa tgctgcacca
     1321 ttgcatttca gccaccccgt tggcggtgtg gctgagggcc atggaatagg tacaccaacg
     1381 ggcagcgccg agccttcccc
//
LOCUS       DBBGLQ010000348         1392 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.354, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000348 DBBGLQ010000000
VERSION     DBBGLQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.354"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..485)
                     /locus_tag="ACIYSB_005692"
     CDS             complement(<1..485)
                     /locus_tag="ACIYSB_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HGC5432464.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     D"
     gene            complement(546..>1392)
                     /locus_tag="ACIYSB_005693"
     CDS             complement(546..>1392)
                     /locus_tag="ACIYSB_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5432465.1"
                     /translation="KRVQAQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLG
                     ELGEKFTKFADENPRVIRGVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIAT
                     AIALAAGLIITNWDVVGPYFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVV
                     KWFQDMWDKLKPIIEWFTDSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPAVDP
                     AQSNNASGATGPNPFMINKASAPKVDGEIKVSFMNSPPGMRVMETRSSGFDVSHDVGY
                     TRFGR"
BASE COUNT          352 a          418 c          312 g          310 t
ORIGIN      
        1 aaatctgcca cgccatcgat actgaaacca ctgaagctgt cactgatgca gtcatccagt
       61 gcagaacagg atgacatcag cgtctgcgcc gtcgccgcac ctgaagtggg gtaagagagt
      121 tctcccgctt cgacaaactt caggtcaaag cggacaatac gcccttcact cttcgatgtg
      181 ctgacccgaa cttccccgtc aacacagact ttcagctcac cgtaagtcgg atggacaagc
      241 gtgccgggac cgggtttatt cagcgcgtca atcaggcgat cgcgctggtc aaagcagtca
      301 tctcccacca cataagctgt gatggacggg cggaaagtga ttttccccag gtcttcggta
      361 tagggtttgt cgcggttcgg gtattcgtgc gtttccacac gacgaccggt tcccgcactt
      421 tcttcttcaa ccttaaacgg cacaccgcga aatgacgcgt cctgaagtct gtctttccac
      481 gtcatataaa ctccgtacat aaaaaatccc accggagtgg gactcattaa cagattaatt
      541 tttcattacc tgccaaagcg cgtatagcca acatcatggc tgacatcaaa accgctggat
      601 cgcgtttcca taacccgcat acccggaggc gaattcataa aagagacctt gatctcacca
      661 tcaacttttg gcgcagaagc tttgttaatc atgaagggat tcgggcctgt ggcaccggag
      721 gcgttgtttg actgagccgg atctaccgcc ggataaggtg tgtatccccg cgccggtatt
      781 cccgtcccat aagcatcata agcacccgcg ccccactgcg cagagttaat ggcatcgacc
      841 gtgtcaccgg aactgtcggt aaaccactca ataattggct tcagcttgtc ccacatatcc
      901 tgaaaccact taacaaccgg cccccagtta ttgatcacca tccccagcgg cgaccaggca
      961 aaaactttct taaggagttc ccagccagcc tcaaaataag gaccaatggt ttcccagagt
     1021 ttcttaaaat aaggtccgac aacatcccag ttagtgataa ttaatcccgc agccagggct
     1081 atcgccgtcg caatcatgcc aatcggcgtc atcgacatga tcctgctgac aatactgatg
     1141 gcaccgccaa cgcccatcaa tcccagtttc agaatcgcaa gaccggcagc aagcccgacg
     1201 acgccgcgaa taacccgggg attttcatcc gcaaacttcg tgaatttttc ccccaactcc
     1261 cccagccatt gcgtgatatt tttagcgtca ccagaaaatg cgccgccaat agctgcaaga
     1321 ccgttagttg cggtccccgt cattgcctcc cacaggttgg acagcgtacc aagctgggcc
     1381 tgaacacgtt ta
//
LOCUS       DBBGLQ010000349         1383 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.355, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000349 DBBGLQ010000000
VERSION     DBBGLQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.355"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            72..455
                     /gene="traM"
                     /locus_tag="ACIYSB_005694"
     CDS             72..455
                     /gene="traM"
                     /locus_tag="ACIYSB_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HGC5432466.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
     gene            642..1331
                     /gene="traJ"
                     /locus_tag="ACIYSB_005695"
     CDS             642..1331
                     /gene="traJ"
                     /locus_tag="ACIYSB_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HGC5432467.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISIQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIARILKISISTSKKHASLICDYFSVSNKDELIILLYNKKFIHYL
                     YEKAMCIINTR"
BASE COUNT          457 a          220 c          261 g          445 t
ORIGIN      
        1 tccctgtttg cattatgaat tttagtgttt cgaaattaac tttattttat gttcaaaaaa
       61 ggtaatctct aatggctaag gtgaacctgt atatcagcaa tgatgcctat gaaaaaataa
      121 atgcgattat tgagaagcgt cgacaggaag gggcaaggga aaaagatgtc agtttttcag
      181 caacagcttc aatgcttctt gaactggggc ttcgtgtaca tgaggctcag atggagcgta
      241 aagagtctgc atttaatcag actgagttta ataaattgct tcttgaatgc gttgtaaaaa
      301 cacaatcatc agtagcgaaa attttgggta ttgagtctct cagtcctcat gtctccggaa
      361 atccaaagtt tgaatatgcc aatatggttg aagatatcag ggagaaggta tcatctgaga
      421 tggaacgatt ttttccaaaa aatgatgatg aataaaagaa atttgacttc gttcaaatat
      481 cagagttttt attatttaaa aaggtgacag tacgaaagat aattagtata ttaattacgt
      541 ggttaatgcc acgttaaaat ttgaatttga aaatcgccga tgcagggagt tctctcctcc
      601 ctgcatcgac tgtccataga atcctttgtg aggaggttcc tatgtatccg atggatcgta
      661 ttcaacaaaa acatgctcgt caaatagatc tgctggaaaa tctgacggca gttattcagg
      721 attatccaaa tccagcctgt atcagggacg aaactggaaa atttattttt tgcaatacgc
      781 tgtttcatga gtcatttctt acacaagatc aaagtgctga aaaatggctt ctgtcgcaga
      841 gagatttttg tgaattgatc tctgtcacag agatggaagc atacaggaat gagcatacgc
      901 atcttaatct tgtagaggat gtttttattc agaacagatt ctggacaata tctatccagt
      961 catttcttaa tggacacaga aatatcattc tgtggcaatt ttatgatgct gctcatgttc
     1021 gtcataaaga cagttataat caaaaaacga ttgtcagtga tgatatcaga aatataatca
     1081 gaagaatgag tgatgattct tctgtatcat catatgtaaa tgatgtcttt tacttatata
     1141 gcaccggaat cagtcataat gctatagcaa gaatattaaa aatatccatc tccacatcaa
     1201 agaaacacgc atctctgata tgcgactact tctctgtttc taataaagat gagttaatca
     1261 tcttactcta caataaaaag tttattcatt atttgtacga gaaggctatg tgtatcataa
     1321 atacgcgtta ataaagtgtt aataaaatat agactttccg tctatttacc ttttctgatt
     1381 att
//
LOCUS       DBBGLQ010000350         1381 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.356, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000350 DBBGLQ010000000
VERSION     DBBGLQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.356"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..608
                     /locus_tag="ACIYSB_005696"
     CDS             <1..608
                     /locus_tag="ACIYSB_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGC5432468.1"
                     /translation="LILVTPVNGLPSSGVADTLTGGFDTEELETWRARVIERYYWTPQ
                     GGADGDYVVWAKEVPGITRAWTYRHWMGTGTVGVMIASSDLINPIPEESTETAARQHI
                     GPLAPVAGSDLYVFRPVAHTVDFHIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKV
                     SRISEAISGANGEYSHQLLAPADNISIAKNELAVLGTISWT"
     gene            599..1183
                     /locus_tag="ACIYSB_005697"
     CDS             599..1183
                     /locus_tag="ACIYSB_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HGC5432469.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            1187..>1381
                     /gene="stfP"
                     /locus_tag="ACIYSB_005698"
     CDS             1187..>1381
                     /gene="stfP"
                     /locus_tag="ACIYSB_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein StfP"
                     /protein_id="HGC5432470.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKARHDQ"
BASE COUNT          305 a          375 c          411 g          290 t
ORIGIN      
        1 cattaatcct ggtcacgccg gtgaatggtc tgccgtcttc cggcgtggca gatacactga
       61 caggtggatt tgatactgaa gagctggaaa cgtggcgcgc ccgcgtcatt gagcggtatt
      121 actggacgcc tcagggcggg gctgacgggg actatgtcgt ctgggctaaa gaagttcccg
      181 gcattacccg cgcatggaca taccgacact ggatgggaac ggggactgtc ggtgtgatga
      241 ttgccagcag cgacctgatt aatcccattc cggaagaatc aacggaaacg gcggcaagac
      301 aacatatcgg gccactggcc ccggtggcag gctctgattt gtatgtgttc aggccggtgg
      361 cgcatacggt ggattttcat atccgtgtga cgccggatac accagaaata cgggctgcca
      421 tcaccgccga gttgcgttcg ttcctgctgc gtgatggtta tccgcaggga gaactgaagg
      481 tgtcgcgtat cagtgaagcg atttccggtg cgaacgggga atacagccat cagttgcttg
      541 caccggcaga caatatctcc attgcaaaaa atgaactggc ggtactgggg acgatttcat
      601 ggacgtgaca aacgatgatt acatccgtct gttgtcggca ctgttgcccc ccggtccggc
      661 gtggtcagcc agcgatccgg cgattgccgg tgcggcaccg tcattaaccc gcgttcatca
      721 gcgtgcggat gccctgatgc gggagctgga tccgcgcacc accaccgaac tgataaatcg
      781 ctgggagcgt ctgtgcggcc tgccggatga atgtattccc gcagggacac agacccttcg
      841 ccagcgtcag caacggctgg atgcgaaggt taatctggcg ggcggcatca atgaggattt
      901 ttaccttgca cagcttgctg ccctgggcag accagacgcc actatcacgc gatacgataa
      961 aagcacgttc acctgctcat cggcctgtac tgacgcagtg aatgcgccgg aatggcggta
     1021 ttactggcag gtcaacatgc cagccgccac caacaccacc tggatgacat gtggcgatcc
     1081 ctgtgattcc gcactgcgta tctggggcga caccgttgtc gagtgcgtgc ttaacaaact
     1141 ctgcccttcg catacctacg taatttttaa atatccggag taatccatgc atcgtataga
     1201 cacgaaaacc gcgcagaagg ataagttcgg cgcgggtaag aacggtttta cccgtggtaa
     1261 cccccagacc ggcacacctg ccaccgatct ggatgatgac tactttgaca tgttgcagga
     1321 ggagctttgc agcgttgtgg aggcatccgg tgccagcctg gagaaggcgc ggcacgacca
     1381 g
//
LOCUS       DBBGLQ010000351         1372 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.357, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000351 DBBGLQ010000000
VERSION     DBBGLQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.357"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            698..1264
                     /locus_tag="ACIYSB_005699"
     CDS             698..1264
                     /locus_tag="ACIYSB_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGC5432471.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHVVFWIDRQRVKKIY
                     PFAERIAECWQAITYNCGSAHRCTYQPHYAYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
BASE COUNT          364 a          277 c          286 g          445 t
ORIGIN      
        1 atcttatcta taatgaacag tattgactta acgatgattg tttatctgaa gtgttttaac
       61 tattctggtt ggaaacaata attctgtcat cacagattaa ctgaatgttt actttttgat
      121 aaggtatcca tgattctgta acgattaaca gagctggata aacaatggaa ttgacaggag
      181 agattcctga ttatgtttgt tctgggttgt attatctaag atgcaggatg aaaatcatgt
      241 cagtggtatt ttttattctg ctatcgtgag ccgtgaatgt tcatgtcctt aagaacagat
      301 tgaatgagcg catataagtt tattgcattg gccttgtaca cggtttttac aaccactgag
      361 agcaagtttg tagtttatga tgtgattggt cgcaatatgt ttcttaacct tctggtcgtg
      421 gtgttttatc gcgtattttg cagtatttcg tgatgtttta ttgagtctgt attttcttta
      481 ctcctcgttt atctcatctc tttagcaaat accatcagat aatccatttc tttctgcata
      541 atgctgcgta tcgttaataa cccgtcgtat ccattctgct acagcatgcc tgataaatac
      601 catctgtaag ttattaccgt tttagatctg attatgagcg aaagcattaa ttcgctcaca
      661 gagcttaaaa catcattaac tttcaggagt catcaacatg cctaaatctt acacaccaaa
      721 ctggtttttt accgctttac ttgacaatca catcaatcaa atgatggcac gctattcctg
      781 cctgcgggcc ttacgcatgg atttcttcta caggaaagat acgcctgatt tcttacaacc
      841 tgatcatcgc tggcttgaat tgcagttgcg tatgctgctg gagcaggtgg aacaatttga
      901 aaatatcgtt ggtttcttct gggtgattga atggacggct gatcatggtt ttcatgcgca
      961 tgtcgttttc tggatcgatc gtcagagggt taaaaaaata tatccctttg cggagcggat
     1021 agcggaatgc tggcaggcaa ttacgtataa ctgtggttcg gcgcaccgct gtacatatca
     1081 gccgcattat gcatacaaca tcaacattcc tgtgcgccac aacgatcctg aaagcatcga
     1141 taatattcgc ggtgccctgc attatctggc gaaagaagaa caaaaagacg ggctgtgtgc
     1201 ctacggctgc aatgaagttc ctgaacgtcc tgctgcaggg cgtcctcgta agcctcactt
     1261 ctgaagctta aggcctgagc ctccgctcct ggaaacactc cgtcggtaaa aacttaccgc
     1321 attgattaat gatgtgaact gaagtcaacg gagatcattc atcctgaacc tg
//
LOCUS       DBBGLQ010000352         1372 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.358, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000352 DBBGLQ010000000
VERSION     DBBGLQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.358"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..911
                     /locus_tag="ACIYSB_005700"
     CDS             <1..911
                     /locus_tag="ACIYSB_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclease PIN"
                     /protein_id="HGC5432472.1"
                     /translation="LWANENLPKASLNASMDGSSSAVYSLNLQAGGYSTAIRQYEEDM
                     PSFTSVSGVYRYWLQYPDEWQNTKEGLKYRIVTNLELAGSQEPGVKTVVTPPQYFTWK
                     NILRCNRVGERYNFTQTNIENIKIEIDRGTAWPGVYTLQLPLKVAYEENKGRYSGQSG
                     GGWPEYAGAIKSFSPVNTNNVTIHLTSKCELTSRYLSINIGDRITPDEARGGINKNAS
                     LSVVCNAPANILFSIRAADMQDGQINKTKCGPGYCTLSFDNDKSQKTVKAIRGTTDVP
                     LSVNFKSDSPVAGLFEGSAVLSMDVL"
BASE COUNT          453 a          210 c          297 g          412 t
ORIGIN      
        1 ttttatgggc aaatgaaaat ttgccgaagg catcacttaa tgcaagtatg gatggttcct
       61 cttctgcagt ttattcttta aatttacagg ctggaggata ttcgacagca ataagacagt
      121 atgaagaaga tatgcccagc tttacatcgg tttccggggt ttacagatac tggctccagt
      181 atcctgatga atggcaaaac acaaaggaag gtcttaaata cagaatagta actaatcttg
      241 aacttgcagg tagtcaggag cctggagtga aaactgtagt aacgccacct caatacttca
      301 catggaagaa tatcttaaga tgtaaccgtg tcggagaaag atataacttt acacagacaa
      361 atattgaaaa tataaaaatt gagattgaca gaggaacagc atggccaggc gtgtatacat
      421 tacaattacc attaaaggtt gcctatgaag aaaataaagg cagatactct ggtcaatcag
      481 gaggaggctg gccagaatat gccggtgcca ttaaatcatt cagtccggtt aatacaaata
      541 atgttaccat acatctaaca tccaaatgtg aacttacatc aaggtatctc agtattaata
      601 ttggtgacag aataacccct gatgaggcgc gaggtggtat aaataaaaat gccagtttat
      661 ctgtagtatg taatgctccg gcgaatattc ttttttcaat aagagctgca gatatgcagg
      721 acggacagat aaataaaaca aagtgtggtc ctggttattg tacgttgtcg tttgataatg
      781 ataaaagcca gaagactgta aaagctataa gaggaacaac agatgttcct ttatctgtga
      841 attttaaaag cgactcgcct gttgccgggc tctttgaagg atctgctgta ctgtcaatgg
      901 atgttctctg aaatctgacc cccaggtttg tattaagagt atttctggtt atgttataac
      961 atatcagtgg tattataagt aataatgatg aagtgtttgt gtttttcctt aatgtctctt
     1021 ttttgagtaa tattgattaa acaatacatt tcacctgttg aattacctgg taactgaagg
     1081 gaaaataaca gaatggatct gcacataaaa agtattttga atgacggagt ttataaaaaa
     1141 tatatgacaa agctgcaaat atgtaagtga tatttattcc ctcgggtctg tttggaggga
     1201 gctaactatt ttaatataag gatcaggtgt aaaatgtatg ggcatcggat agtaaactta
     1261 aatgtaagct acgatcgtca ccttattgat aaggttgttg ttcaacttga gctgaatggt
     1321 gaggcaacat ttgaatgtgt tctttattca gagggcgaag gtgattttcg aa
//
LOCUS       DBBGLQ010000353         1367 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.359, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000353 DBBGLQ010000000
VERSION     DBBGLQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.359"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..373
                     /locus_tag="ACIYSB_005701"
     CDS             <1..373
                     /locus_tag="ACIYSB_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGC5432473.1"
                     /translation="EPSSLATMILEAWCSALHLEAWQGDGLVYFSKWTPSRKPASSDA
                     RPSSDDTPLSGGCSDTRKASEKKPGEAAVLWIKRGDVEAMQKARNRASYLVKYETKQH
                     DGSGQRNYGCSRGPGRLLDGR"
     gene            883..1335
                     /locus_tag="ACIYSB_005702"
     CDS             883..1335
                     /locus_tag="ACIYSB_005702"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGC5432474.1"
                     /translation="MNVIDSRIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILSTEICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
BASE COUNT          338 a          277 c          391 g          361 t
ORIGIN      
        1 cgaaccatct tcgctggcga cgatgatcct ggaggcatgg tgcagcgccc tgcatcttga
       61 ggcctggcaa ggcgatggcc tggtctattt ttccaagtgg acgccttccc gtaaaccagc
      121 atcgtctgat gctcgccctt cttccgatga cacgcctttg tcgggtggat gttctgatac
      181 caggaaggct tcagaaaaaa agccgggtga agccgctgtt ctctggatca agcgtggtga
      241 tgtggaagcg atgcagaaag cccggaacag agccagttat ctcgtgaaat atgagacgaa
      301 gcagcatgac ggttccgggc aacgtaatta tggttgcagt cgtgggccgg ggcgtttgct
      361 ggatggcagg tgaaccgggt aaaacggcat ccggcgcctg agcgtatacc acggtaaggt
      421 cgttggctga cgcccttagc tcgttttctg aaaaagtcgt cctgaagtca tgtatctgct
      481 tccattctca tgttcatatt tctctgacgc tttcactgtc tgtcatcgga agtaccgatt
      541 tattttggtg ctgcacactt acggccattc acggaagagg attgtggtga acagtaggag
      601 tctggctgct ggcgagggaa ggttcaggac tggtatgggg tttagtgctg gaggatgact
      661 ttgaatcccg gattgaccag tgagggggca gtgagaggat gctctggtgt aacaggcctg
      721 gattgcaaaa tataaaaccg gttgtacact cgtaacgggc aaaaggatac gagcaataac
      781 acatggtacc cggtgagact ggctcttgta tcacattacc gggaatattc tgacaggtga
      841 aatgttctga taagaatcat cctgagggag gatacaacgg atatgaacgt tattgacagc
      901 cgcattcgtg atgtgaaatt tggtgaaaat gtgaaagtcg tcatgccagt aaacctctat
      961 gaatgtgaac tgggggatga tgtttttatc ggtccctttg tggaaataca gaagggatgt
     1021 atcattggca accggagccg aattcagtcc cacagtttca tctgtgagaa cgtcatttta
     1081 ggtgatggct gtttcatcgg tcatggcgta acttttgcta atgatctttt taagtgtggt
     1141 ggtcccgatg cctctgcaga aagctggata cgcatcgtat taggtaacac tgtaacagtg
     1201 ggtagcggag cgaccatttt aagcacagaa atttgcagtg gagcagtgat tggtgctggt
     1261 agtgtggtta caaagcccgt gctggtaaaa ggcgtgtatg ccggtaatcc agccagactt
     1321 ttgagaacac tttaattata tttaactcaa taataaatca gacagtg
//
LOCUS       DBBGLQ010000354         1363 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.360, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000354 DBBGLQ010000000
VERSION     DBBGLQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.360"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(150..575)
                     /locus_tag="ACIYSB_005703"
     CDS             complement(150..575)
                     /locus_tag="ACIYSB_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGC5432475.1"
                     /translation="MNGIKTGLGMTPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSAQQKAACVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRATCTKSVYSYQPDLFQDCGGAST"
     gene            complement(572..955)
                     /locus_tag="ACIYSB_005704"
     CDS             complement(572..955)
                     /locus_tag="ACIYSB_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGC5432476.1"
                     /translation="MYAKSFLAFDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
BASE COUNT          331 a          355 c          364 g          313 t
ORIGIN      
        1 taagttggag tcattaccta ttcaggtaag caggattgcg gatatccggt gtcgcgcctt
       61 tgtgtcacga acggggtagg tgcgaaacac cggataaaat gcaggctggc aatacctctg
      121 aacgccctgc gcagagcgga tagtttggat taagtactcg cacctccgca gtcctgaaac
      181 aagtctggct ggtagctgta aacagacttc gtacatgttg ctctggaata gatccccgtg
      241 ccacaggctt cgcagaactt tttcccggga aaatgctgcc cgcacatcac acaatgccac
      301 tccagcacga ccggtaatgg cgatagaaac atcgccatat cctcaatgta agggtgggac
      361 ttttccggat tcagcaccac gcaggccgcc ttctgttgcg cgctcagggc atgtaaatca
      421 tgctcaaacc acgccccctg agcatctgtc tgcaaaatca accggccacg acaggaaagg
      481 cagaaacact gccggatatt tctgctgagc gcagagtcgg cactgataat gtgttcaccc
      541 ggcgtcatcc ccagccccgt ttttataccg ttcattcagc cactccctcc tcactgaagt
      601 gccctgtatg gcagtgagtg cagtaccgct ccccataata atcgtgatga cattgtctgc
      661 agtgccagct ggctttacgc accacgggta aggcatccgg tacgaattgc tgcagacgtt
      721 taatcaactg tatttctctg cgctccggcc tgacataagg gcactgttga ccgtgctctg
      781 tcagtctgtc gtcagtgtgt tcaaaccagg gaagttcagt ttcgtattgc ggatggtatc
      841 tgagtgcact gccacacaag tggcaggtgt agttagcata aggtgcggcc tgtgcagtac
      901 gggcaccggt caggcgtccg ttgccatcga atgcgagaaa agattttgcg tacatcgtca
      961 tattcctcag ccgccagacg acacgcaggc aacagccgtc ccggtacggg cagtgtggtc
     1021 aggtgtgaat ggcggttcag ttaaaaatct ggaatggggg tgaggtaaaa caggcaggct
     1081 gctgttacag cactgtggtt gtcacacaat ggcccaccga cgtaaaaaac tgtctgcaag
     1141 cagagatgga catcagagaa tgtgctgtat cggttgccgg aaggactggt aaagaatgtg
     1201 ccatggttaa atctccgtat ggttcatacg gaatagccat gtcgtaaaaa atgtgcagtg
     1261 ccgctgaagt ggcctccgga accgactgca aagattagag ccagtccgca gttcttgctc
     1321 agaaccttat acctgagaac atatccccgg cagggtgctg tcc
//
LOCUS       DBBGLQ010000355         1362 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.361, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000355 DBBGLQ010000000
VERSION     DBBGLQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.361"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..534
                     /locus_tag="ACIYSB_005705"
     CDS             <1..534
                     /locus_tag="ACIYSB_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGC5432477.1"
                     /translation="ALISEPGDIRRFIQQAVDHWPRLLAVHFTLHSAEGNINGQQIHA
                     FCTSFYRQVHERITERNHTASPAPPVVLRWLREQHGGATMRCLLLLSQELFCHPRASV
                     TVDEECSQLVDLLQQTWQVISAGGQCRVEKRFQVVRGDTSGQYVALKTVALSLGLPVV
                     IAITHRPVQRCTLITAQ"
     gene            complement(553..1032)
                     /locus_tag="ACIYSB_005706"
     CDS             complement(553..1032)
                     /locus_tag="ACIYSB_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGC5432478.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKTNTVHNEKLLRVRNQTPWR
                     HMTKRQRKNRKRINRLVELWPELFNREKPKPLKVGITDDLIQDIAIRELAFGAGALRA
                     AVASYVQSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLMVLNDRRKARKPR"
BASE COUNT          310 a          340 c          366 g          346 t
ORIGIN      
        1 gccttaattt cagagccggg agacatccgg cgttttattc agcaggcggt ggatcactgg
       61 ccgcgtctgc tggccgtcca cttcacactc cattcggcag aaggaaacat caacggacaa
      121 cagattcatg cattctgtac ttccttttac cgacaggtgc acgagcgtat tactgaacgt
      181 aatcacacgg ccagtccagc accaccggtg gtattacgct ggttgcggga acaacatgga
      241 ggagcaacaa tgcgatgcct gttgctgctc agccaggagc ttttctgcca tccgcgagcc
      301 agtgtgacag ttgatgaaga atgttcacaa ctggtggatt tactacaaca gacttggcag
      361 gtgataagcg ctggcggaca atgccgggtg gaaaagcgtt ttcaggtcgt ccggggtgat
      421 acatctggcc agtatgttgc gttaaaaaca gtcgcattgt ctctggggtt accggttgtg
      481 atcgccatta cccatcgtcc ggtacagcgc tgtacattga ttacagctca gtgaatcagc
      541 gcagttgtgc agtcaacgag gttttctggc ttttcgtcgg tcattcagca ccattagccg
      601 ggtctctgcc tctttctgtt cctgtggagt cacttccccg cacggctgcc ccttcaggtc
      661 ataacgggcg ccgccagcca ttaacgcacg gtaatagcgc ggagactgca cataagacgc
      721 tactgccgcg cgtaatgcac ctgcaccaaa cgccagttct cggatggcga tatcctgtat
      781 caggtcgtca gttatcccca ctttaagcgg ttttggtttt tcccggttga ataactcagg
      841 ccagagttcc acaagacgat ttatacgctt acggtttttg cgctggcgtt ttgtcatatg
      901 tcgccatggt gtttgattcc tcacccgaag aagcttctcg ttgtgtactg tgttcgtttt
      961 atcctgcagc ggcgttgccg gtgatttact gaaaataccc tgcggtttac ggttaatggt
     1021 cagcgttacc atcagtattg ttacctcgtg ctgtcaatgg aagtggtacc tcatccggta
     1081 tctgaaaatg ttttccggtg gatggcacag cgaatgtcat gtaagacatg ctgttgccgg
     1141 tattgttcag tgctgtgcgt agtgagcgta ttcgttatca gacagagggg cagggaacaa
     1201 atggcgtaaa cgggcatcaa agagccaaag aaacagtaca ggtcaggccg ttatggtctg
     1261 caaacagagt cctggcgccc ccgggactga aaacgaaaca ataacccgcg cacagtgcga
     1321 ggagcccggg cgtgaagcaa tcagcaggta atattaatgg ca
//
LOCUS       DBBGLQ010000356         1350 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.362, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000356 DBBGLQ010000000
VERSION     DBBGLQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.362"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..447
                     /locus_tag="ACIYSB_005707"
     CDS             <1..447
                     /locus_tag="ACIYSB_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGC5432479.1"
                     /translation="DPSRFLFVLSHADRVFPAEEWNDTEKCPSRHQELSLATVTARVA
                     TLFPSSFPVLPVAAPVGWNIPALVSLMIHALPPQATSAVYSHIRGENRSERVQKHAQQ
                     TFGETIGKSFDDAVARFSFPAWMLQLLRKTRDRIIHLLVTLWDHLF"
     gene            566..1243
                     /locus_tag="ACIYSB_005708"
     CDS             566..1243
                     /locus_tag="ACIYSB_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432480.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGNRISINGNLIPTSQMCNQQTGFRAVEVEVGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEEITETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATGA
                     SVEFIGK"
     gene            1249..>1350
                     /locus_tag="ACIYSB_005709"
     CDS             1249..>1350
                     /locus_tag="ACIYSB_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGC5432481.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDD"
BASE COUNT          324 a          364 c          342 g          320 t
ORIGIN      
        1 gatccctcgc gttttctgtt tgttctcagc catgccgatc gcgtatttcc tgctgaagaa
       61 tggaacgaca cagaaaaatg cccgtcccgt caccaggaac tctcgctggc gacggtgaca
      121 gcccgggtgg caaccctgtt cccttcatca tttccggtac tccccgttgc cgcgcctgtc
      181 ggctggaata ttccggcact ggtgtcactg atgatccacg cactgccgcc acaggcaacc
      241 agcgcggttt attcacatat ccggggggaa aaccgctctg aacgggtcca gaaacacgca
      301 caacagactt ttggtgaaac catcgggaaa agttttgacg acgccgttgc ccgtttcagt
      361 tttccggcct ggatgttaca gcttctgcgc aaaacccggg accgcattat ccacctgctg
      421 gtcacactgt gggatcacct gttctgaacg ctccggagtc ccccgtggca accgtatacc
      481 tttacacgac aacggattac ggtaaccact gccgtatacc gtctgtcatt atcaataaca
      541 cgctctttaa tttcagaggt cagtcatgaa taccagaatt ctttttcccc tgctgttcac
      601 tgtggcatca ttctccgcct ccgccggcaa ctgggctgtc aaaaacggct ggtgtcagac
      661 catgacggaa gatggtcagg cgctggtaat gctgaaaaat ggcacgattg gtattaccgg
      721 cctgatgcag ggatgcccga atggcgtaca gacgctcctg ggcaaccgta tcagcattaa
      781 cggtaacctg atccccacat cacaaatgtg taatcagcag acaggattca gggctgttga
      841 ggtggaagtc ggacaggcgc cggaaatggt caaaaaagcc gttcactcca tagcagagcg
      901 tgatgtgtcc gttttacagg catttggtgt acgaatggaa ttcacccgcg gtgatatgct
      961 gaaggtctgt ccgaaatttg tcacatcact tgccggtttt tccccgaaac agacgaccac
     1021 tattaataaa gattccgtcc tgcaggctgc ccggcaggca tacgcccggg aatatgacga
     1081 ggaaataaca gaaaccgctg attttggctc ttacgaagta aaaggcaata aggttgagtt
     1141 tgaagtgttc aatcctgaag accgtgcgta cgacaaagtg accgtcacgg ttggtgctga
     1201 cggtaatgcc accggcgcca gcgttgaatt tatcggaaaa tagccggtat gtcggactgt
     1261 caccctgttt tattgcccga aggccctttc tcacgcgaac aggcgatggc tgtcacaaca
     1321 gcttaccgca atgtgcttat tgaagatgat
//
LOCUS       DBBGLQ010000357         1338 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.363, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000357 DBBGLQ010000000
VERSION     DBBGLQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.363"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>1338
                     /locus_tag="ACIYSB_005710"
     CDS             <1..>1338
                     /locus_tag="ACIYSB_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432482.1"
                     /translation="SGYVVRVCAKDMKVPGISVSVVGRAKAAKASKELSVEPTETTVN
                     PGDTLSVGVASTATTQSVSFTPKETPQIKGEEKALFFIKDGDASQNRTLSLTRDDEES
                     ELFTLTLKEKQTDGSIEVLESHQVSFNPEGTNDMGQPAWIPTLLESQSTRIGAVLADN
                     AEASAAQLIFEDVAFEGGTDGDLSEIDTEDYLEALKVLEASEVNYTALLSLGCYDASA
                     LAAIKKLAEDVRVDMFYDLKGNQTPENAISEAKSHSFGGSHQPSRYYFPLSCRDTFTG
                     MNVVYGISCDAFVAKAKGVALVPDVGGWHYAPAGISRAIIDRQNIARIPNIGAVDREA
                     FVLARINPVSVAADGSVYIDDSLTTYSKNNYLRFQHVSSLMNAIARDFYEVAQAIKHE
                     PDGITKETLMKAMTELLDRYVAAGALVTPRDKSQGEDPYVVQVVQKDIDLWEVS"
BASE COUNT          372 a          308 c          328 g          330 t
ORIGIN      
        1 tctggatatg tcgttcgagt ttgcgctaaa gacatgaagg tgccgggtat ttctgtttcc
       61 gttgtgggca gagcaaaagc ggcaaaggcg agtaaagaac tttccgttga accaactgaa
      121 accacagtaa atccaggtga cacattaagc gttggtgttg cttcaactgc aacgactcaa
      181 tcagtgtcct tcacacccaa agagacgccc cagattaaag gtgaagagaa ggcattattc
      241 ttcatcaaag atggtgatgc atctcaaaat cgcacgttat cactaactcg cgatgatgaa
      301 gaaagcgaac tctttacgct gacgctgaaa gaaaaacaaa ccgatgggtc cattgaggtg
      361 ctggaaagcc accaggtgtc tttcaatcca gaaggcacta acgacatggg ccaaccagca
      421 tggattccga cactgcttga aagccagtcc actcgaattg gtgccgtact ggcagataat
      481 gcagaagcat ccgctgctca actcattttt gaggatgtag cttttgaagg tggtactgat
      541 ggagatctgt ccgaaattga cactgaagac tatctggaag cattaaaggt tctcgaagct
      601 tcagaggtta attacaccgc attgctgtca ctgggttgtt atgacgcatc cgccttagcc
      661 gcaatcaaaa agctggctga agatgttcgc gtagacatgt tctatgacct gaagggcaac
      721 cagacacctg aaaacgctat ttcagaagcg aaaagccata gttttggcgg ctcacatcaa
      781 ccaagccgtt actacttccc gctctcctgc cgagatactt tcactggaat gaatgtcgtc
      841 tatggcatta gctgcgacgc attcgtagcg aaagcaaaag gagtggcgct ggtaccggat
      901 gttggtggtt ggcattacgc accggcaggt atatcacgcg cgattattga tcgacagaac
      961 attgcccgaa ttccaaatat cggtgcagtt gatcgtgaag cgttcgttct tgcgcgcatt
     1021 aatccagttt cagttgctgc tgacgggtcc gtttacattg acgactcttt aacaacttac
     1081 agcaaaaaca actatctgcg attccagcat gtgtcttcct tgatgaacgc catcgccaga
     1141 gatttctatg aagtagctca ggcgattaag cacgaaccgg atggcattac caaagaaacg
     1201 ctaatgaaag caatgactga attgcttgac cgttacgttg cagccggtgc gctggttact
     1261 ccacgtgaca agtcccaggg tgaagatcca tatgttgttc aggttgtcca aaaggacatc
     1321 gatctgtggg aagtgtcc
//
LOCUS       DBBGLQ010000358         1331 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.364, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000358 DBBGLQ010000000
VERSION     DBBGLQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.364"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..1119
                     /locus_tag="ACIYSB_005711"
     CDS             <1..1119
                     /locus_tag="ACIYSB_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5432483.1"
                     /translation="LNRGFTVYTPDTQVQEKDGRVYWQLKNNAGLADSQAQPVSENEI
                     RKVAADGDSGNNRADESGSAVSGKNSEVTVKGSSLFKGADSIYLLKKARTMFAAREFI
                     LSDSADRWTQVVDNSDADGGAWAMTGYSHGGYDDFSLNQSGLNVGFRHSSAGNAWWGM
                     GIEFYRGHSSTDDYRDDFSLWGVHVLAGKSFAAGLFVDGMTGYRELSEDYTIQGELSD
                     LSGRAKSHILTAGIRGGWKMHASPLDMSITPTVSLNGARVDGNRLQGRERSVELYDGD
                     ALWLKAGFEAEKVSGNMTLKAGIWRNITLKDMPGMTLRDDWKARHYDAEKADRYTVSF
                     GINGKLTEKLSVQAKVNSSFDGYFKTDAEGILGIRYSF"
BASE COUNT          343 a          275 c          412 g          301 t
ORIGIN      
        1 ctgaacaggg ggtttactgt ttatacccct gacacacagg tgcaggagaa agacggcaga
       61 gtttactggc aactgaaaaa taacgctggt ctggcggact cacaggcaca accggtatcg
      121 gaaaatgaaa tccggaaggt tgcggcggat ggtgatagtg gtaacaaccg cgcggatgaa
      181 tctggttccg ctgtttccgg aaagaactca gaggttacag tgaaaggcag ttccctgttt
      241 aaaggggcag atagcatata tcttctgaag aaggcgcgaa ccatgtttgc cgcccgtgag
      301 ttcattctca gtgacagtgc tgaccgctgg acacaggtgg tggataacag tgatgcagac
      361 ggcggtgcat gggctatgac agggtacagt catggtggat atgacgactt cagcctgaat
      421 cagagcggac tgaatgtggg attccgccac tcttctgccg gtaatgcctg gtgggggatg
      481 gggatagagt tttaccgggg gcacagcagc acagatgact accgggatga tttcagcctg
      541 tggggagtgc atgttctggc gggcaaatcc tttgccgctg gtctgtttgt ggatggtatg
      601 accggatacc gggagctgtc tgaagattac actattcagg gagagctgag tgacctgtcc
      661 ggcagggcaa aaagtcatat cctgacagcg ggtatccgtg gtgggtggaa gatgcatgca
      721 tccccccttg atatgtcgat aacaccgact gtttcactga acggtgcccg ggtggacgga
      781 aatcgtctgc agggccggga gcggagcgtg gagctttatg atggcgatgc cctgtggctg
      841 aaagccgggt ttgaggcaga aaaagtatcc ggaaatatga cgctgaaggc aggtatctgg
      901 cggaatatca ctctgaagga tatgcccgga atgaccctgc gtgatgactg gaaagcccgt
      961 cactatgatg cagagaaggc tgaccgctat accgtttcgt ttggtatcaa cggaaagctg
     1021 acagagaaac tgtcagtgca ggcgaaagta aacagcagtt ttgatggtta ctttaagacg
     1081 gatgcggaag gaatactggg tatcaggtat tccttctgat aacagccatc gatgatgctg
     1141 cagagagaag gaggggtaga atatagcccc tccggtgagc cggacgtaag agtgtactga
     1201 ctccaaaaag ttggacagtt gtagttgagc acaaaatcag gacaccggga taacctgttt
     1261 ccacttttct tcatgtagcc aaggcggtaa accatcgttg ttcgtgtgag gtcgataaac
     1321 gttataataa c
//
LOCUS       DBBGLQ010000359         1299 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.365, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000359 DBBGLQ010000000
VERSION     DBBGLQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.365"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            108..413
                     /locus_tag="ACIYSB_005712"
     CDS             108..413
                     /locus_tag="ACIYSB_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndB"
                     /protein_id="HGC5432484.1"
                     /translation="MSNSIEIHAIKVTQPYGEFFQTVMSAEDLLKCIYVNQAEMDDGE
                     ISGVQRKESMPRALDIAKFIDSDEAAFPNSIILSVNHNEDGTPAKEDDKWEFKKSEN"
     gene            462..1268
                     /locus_tag="ACIYSB_005713"
     CDS             462..1268
                     /locus_tag="ACIYSB_005713"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HGC5432485.1"
                     /translation="MDGQHRLLGFKYAKNKDIQLPCSIYNSLPPSNQATIFSTINFNQ
                     QKVPKSLAYRLFGYSLDDISREYWPPDLLAVYFSEKFNKTDEYPFYRRLKNRVLNEIT
                     ENDWSISSSVFIDGVLSLISKNPRADRYTINAIDSDEKEKGRGRLDNKNSKDKSPLRW
                     FYIKGNDKAIEQILKIYFSAIKDHFWANVCIEKGTVLVRSVGISALFQFLRKKLMDMP
                     KINKENIEKLCSALKTVNPEEFTKNTEYTSTTVGQRKIYDYLNENVKTDF"
BASE COUNT          475 a          190 c          239 g          395 t
ORIGIN      
        1 ttaccgttgt taagcctgat tttaaaatcc ctgtaactcc agattcatta gtgtctgatg
       61 aattaaggaa tatgagttct aatgatgagc caggtgaggg gctataaatg tctaattcta
      121 ttgagatcca tgcaattaag gtcacccaac catacggcga attctttcag acggttatgt
      181 ctgccgaaga tttattgaaa tgtatttatg tgaatcaggc ggaaatggat gatggggaaa
      241 tatcaggtgt gcagaggaaa gagtctatgc caagagcctt agacatagca aaattcatag
      301 actccgatga ggctgcattt ccaaactcaa tcatactctc tgtcaatcat aatgaagatg
      361 gtacacctgc aaaggaagat gacaaatggg agtttaaaaa aagtgagaat tgattatata
      421 agataataat accgaatata aatctaagat tatgttctat tatcgatgga cagcacagat
      481 tattaggatt taagtatgct aagaataaag atattcaatt accttgttca atttataact
      541 cattaccacc atccaatcaa gcaactatat tttcaacaat taattttaac caacaaaaag
      601 ttcctaaaag tttagcatat agactctttg gatactctct tgatgatata tccagagagt
      661 attggcctcc tgatttgctt gctgtatatt ttagtgagaa attcaataaa acagatgaat
      721 atcctttcta cagacgatta aaaaatagag tattgaatga aattactgaa aatgactggt
      781 ctatatcatc gtctgtattt attgatggtg tattatcact aatatctaaa aaccctcgtg
      841 ctgatagata taccatcaat gcaattgatt cggatgaaaa agaaaaagga aggggcaggc
      901 ttgataataa aaacagcaag gataaaagcc cattgcgatg gttttatata aaaggcaatg
      961 ataaggcgat agagcaaata ttgaaaattt atttttcagc gataaaagat catttttggg
     1021 ctaatgtatg tattgagaaa ggcacagtgt tagttagatc tgtcggtatt tctgcattat
     1081 ttcagtttct acgtaaaaaa ttgatggaca tgcctaaaat taacaaagaa aatattgaaa
     1141 agctgtgctc tgcattgaaa actgtgaatc ctgaggagtt tacaaaaaat acagagtata
     1201 catcaacaac tgtgggtcag cgaaaaatat atgattattt aaacgaaaat gtcaaaacag
     1261 atttttaaaa ttgactaaat tttgttcagg ccatccata
//
LOCUS       DBBGLQ010000360         1295 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.366, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000360 DBBGLQ010000000
VERSION     DBBGLQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.366"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(27..626)
                     /locus_tag="ACIYSB_005714"
     CDS             complement(27..626)
                     /locus_tag="ACIYSB_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGC5432486.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(693..>1295)
                     /locus_tag="ACIYSB_005715"
     CDS             complement(693..>1295)
                     /locus_tag="ACIYSB_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGC5432487.1"
                     /translation="RLFAVSITSSGNPPAFSLTPDGRLTAKNADISGNVNANSGTLNN
                     VTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQ
                     IVIPAVAFRGARHPRKDKEPYSSCRLIVKKNGAEIYNRTALDNTLIYTGVIDMPAGSG
                     VMTLEFSVSAWWVNGWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          304 a          379 c          307 g          305 t
ORIGIN      
        1 gtcataacac tgtgttacct ggctaatcag aatttataac caacacccac gatgaacccg
       61 tcagtgcgcc agtcaccact gccggagcct tcataagcga catcaatggc cacggattcg
      121 gtcgggttaa actgcacgcc agctccccac gccagagacg tgttgctgtg gcgaccgtca
      181 tcacttccgg tcagcacatc gtgctttttc cccttgttgt cagttacgcg gagataatcc
      241 ccggagaaag tcgacacacg gctgtaagcc acacccgcca tcgcatacgc gctgaaccat
      301 tcattcacgc gcacagacgg ccccgccatt acgctgaacc agcggttacg aacggaatct
      361 tcatgccagc gggtatcgct gtaacgggta atctggcgat tcttgtctcc tgcatagctg
      421 aacgacgtca ccatccccag tgtgtccgta aactcataac ggtatttcac gttaatcccg
      481 ttcagatcat cactgccggg aacgttcgtc cgggcatgaa gataccccgc gctcagcgtg
      541 gactgatgtt cagacgccca tgcaggcgca ccggatacgg ccagacaaat ggctgcggac
      601 aaaatggcgg cataaagttt acgcataatt acctctcgct tttctgcaat aaaaaaggcg
      661 tcatttctga cgcccgttct gggttataaa attcagctaa tcgtgatgcc tgcagtggct
      721 ttcttcatca caacaacaag aaggtcactg atacttgctg tgggatacca gccatttacc
      781 caccatgctg ataccgaaaa ctccagtgtc attacgccac tgcctgcagg catatcaata
      841 acacccgtgt atatcagcgt attatccaga gccgttcggt tataaatttc agccccgttt
      901 ttcttcacta tcaggcggca tgacgaataa ggctccttat ccttccgcgg atgtctggcc
      961 cccctgaaag ccaccgctgg aataacaatt tgccggtcaa acggctgatc gtcataaacc
     1021 ctgacggtaa tggtccctga tggccaccgt tccggtgcac gggagtcccg ggggaaagct
     1081 ttacccactg ttttaacgag atcgccttca atctggttcg cggacagttt tcccagaacc
     1141 cggcagttct cgttaatcgt gacgttgttg agcgtcccgg agttcgcatt cacgttaccg
     1201 ctgatatcgg catttttcgc cgtcagacgc ccgtccggtg tcagggaaaa tgccggggga
     1261 ttgccggatg aggtgatact caccgcaaac agtcg
//
LOCUS       DBBGLQ010000361         1291 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.367, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000361 DBBGLQ010000000
VERSION     DBBGLQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.367"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            32..1144
                     /locus_tag="ACIYSB_005716"
     CDS             32..1144
                     /locus_tag="ACIYSB_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HGC5432488.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT          323 a          314 c          352 g          302 t
ORIGIN      
        1 ggtattacgc ctgatatgat ttaacgtgcc gatgaattac tctcacgata actggtcagc
       61 aattctggcc catattggta agcccgaaga actggatact tcggcacgta atgccggggc
      121 tctaacccgc cgccgcgaaa ttcgtgatgc tgcaactctg ctacgtctgg ggctggctta
      181 cggccccggg gggatgtcat tacgtgaagt cactgcatgg gctcagctcc atgacgttgc
      241 aacattatct gacgtggctc tcctgaagcg gctgcggaat gccgccgact ggtttggcat
      301 acttgccgca caaacacttg ctgtacgcgc cgcagttacg ggttgtacaa gcggaaagag
      361 attgcgtctt gtcgatggaa cagcaatcag tgcgcccggg ggcggcagcg ctgaatggcg
      421 actacatatg ggatatgatc ctcatacctg tcagttcact gattttgagc taaccgacag
      481 cagagacgct gaacggctgg accgatttgc gcaaacggca gacgagatac gcattgctga
      541 ccggggattc ggttcgcgtc ccgaatgtat ccgctcactt gcttttggag aagctgatta
      601 tatcgtccgg gttcactggc gaggattgcg ctggttaact gcagaaggaa tgcgctttga
      661 catgatgggt tttctgcgcg ggctggattg cggtaagaac ggtgaaacca ctgtaatgat
      721 aggcaattca ggtaataaaa aagccggagc tccctttccg gcacgtctca ttgccgtatc
      781 acttcctccc gaaaaagcat taatcagtaa aacccgactg ctcagcgaga atcgtcgaaa
      841 aggacgagta gttcaggcgg aaacgctgga agcagcgggc catgtgctat tgctaacatc
      901 attaccggaa gatgaatatt cagcagagca agtggctgat tgttaccgtc tgcgatggca
      961 aattgaactg gcttttaagc ggctcaaaag tttgctgcac ctggatgctt tgcgtgcaaa
     1021 ggaacctgaa ctcgcgaaag cgtggatatt tgctaatcta ctcgccgcat ttttaattga
     1081 cgacataatc cagccatcgc tggatttccc ccccagaagt gccggatccg aaaagaagaa
     1141 ctaactcgtt gtggagaata acaaaaatgg tcatctggag cttacaggtg gccattcgtg
     1201 ggacagtatc cctgacagcc tacaaaacgc aattgaagaa cgcgaggcat cgtcttaacg
     1261 aggcaccgag gcgtcgcatt cttcagatgg t
//
LOCUS       DBBGLQ010000362         1283 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.368, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000362 DBBGLQ010000000
VERSION     DBBGLQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.368"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..125
                     /locus_tag="ACIYSB_005717"
                     /pseudo
     CDS             1..125
                     /locus_tag="ACIYSB_005717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004722843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(579..1142)
                     /locus_tag="ACIYSB_005718"
     CDS             complement(579..1142)
                     /locus_tag="ACIYSB_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGC5432489.1"
                     /translation="MDIIEKRITKRHLSESKLSGVNYYNCIFERIQLDNFNFRDCEFE
                     KCRFVNCSIKNLKLNFFKLIDCEFKDCLLQGVNAADIMFPCTFSLVNCDLRFVDFISL
                     RLQKSVFLSSRFRDCLFEETDLRKSDFTGSEFNNTEFRHSDLSHCDFSMTEGLDINPE
                     INRILSIKIPQEAGLKILKRMGVVVGG"
BASE COUNT          387 a          258 c          236 g          402 t
ORIGIN      
        1 gtgatgtact gatagtctgg aaactggatc gacttggtcg ttcactctca catttgctcc
       61 gaattattga agacctgaaa attcgtggca ttgctttccg atcattgact gaatctattg
      121 atacaagggg tttgaggccc aaccgtacga gatcgtacgc taagttcatt ttttgagcag
      181 aagaggtgag taaagaaaac gagggttttg tttactcttc ccgtcaatgc cagtagccat
      241 gcggtttcag ggactttcag tgttaataaa ctcggtaatc aaatcaacgt aaacctgcct
      301 aacaacgcca cgactttatt gacttaaaat catatagtta gcttcttagc gtacgtttcc
      361 gctccattgg gcttcaaacc ccacacccaa tgacatgaga aaagctggat ctgattctca
      421 aaatgctggg atacccatcg agatggagaa ctcagtcaat gttccattct ttctgaaata
      481 tgtgtgggga cactgatctg tcgggagccg gatgcggtag ttccgcaggt ccggttctga
      541 ggaggggccc gtccgggcaa ccggcgggtc tactcacctc atccccctac aaccactccc
      601 attcgtttaa gtatttttaa acctgcttcc tgtggtatct ttatagataa gatcctgttt
      661 atctcaggat ttatatcgag accttctgtc attgaaaaat cacagtgaga caaatcagaa
      721 tgtctgaact ctgtattatt aaactccgaa ccagtaaaat cagatttacg aaggtctgtt
      781 tcctcaaata aacaatcacg gaagcgactg gataaaaaaa cagatttctg taaccgtaaa
      841 ctaataaaat caacaaatct caaatcacaa tttacaagag aaaaagtaca gggaaacata
      901 atgtctgctg catttactcc ttgtaataaa caatctttga attcacagtc aataagttta
      961 aaaaaattaa gttttaaatt ctttattgag cagtttacga agcgacattt ttcgaactca
     1021 caatcgcgaa agttaaagtt gtcaagctgt attcgttcaa atatacaatt gtaataattt
     1081 acacctgata atttactttc actgaggtgt cgttttgtga ttcttttttc tattatatcc
     1141 atgtgtcagt cccctgttcc cataacttct ttaataaatg ccttttctgg agttgtttca
     1201 ttattggatt gaataaaatc actcatatca gaataacgat ttatactatt ttttgaaagc
     1261 atatagaagg ggattttatc tct
//
LOCUS       DBBGLQ010000363         1274 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.369, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000363 DBBGLQ010000000
VERSION     DBBGLQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.369"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..312
                     /locus_tag="ACIYSB_005719"
     CDS             <1..312
                     /locus_tag="ACIYSB_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432490.1"
                     /translation="GVDAGRALLNWQPHTILDHAHYMELWTGDRLAAGSAREGVNPGI
                     LATYGDGGIVKTDEPGHKVPSSLRAISSRNVKRFGEISGNVGTETRPRNVAFNYIVRA
                     E"
     gene            323..784
                     /locus_tag="ACIYSB_005720"
     CDS             323..784
                     /locus_tag="ACIYSB_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432491.1"
                     /translation="MGEGSALPVGVPVPWPSATPPTGWLKCNGAAFSSEKYPNLAKAY
                     PTNKLPDLRGEFIRGWDDGRGVDAGRQLLSSQGDAIRNIEGFADGGIGMSFDAIRGAF
                     YDAGTRSARMPNNTTDIGKTDDLGFDASRVVPTANENRPRNIAFNYIVRAA"
     gene            795..1262
                     /locus_tag="ACIYSB_005721"
     CDS             795..1262
                     /locus_tag="ACIYSB_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432492.1"
                     /translation="MGEGSALPVGVPVPWPSATPPTGWLKCNGAAFSSEKYPNLAKVY
                     PTNKLPDLRGEFIRGWDDGRGVDNGRNLLSAQSDAIQNIVGTFGRTQLFKDALNSGPF
                     SQTDSILSVGLQPTEILEGYGASVWTFDASRSVRTASETRPHNIAFNYIVRAA"
BASE COUNT          327 a          281 c          341 g          325 t
ORIGIN      
        1 ggggtggatg ctggccgcgc tttgcttaat tggcagccac acacaatttt ggatcatgca
       61 cactatatgg aattatggac aggggacaga ctcgccgcag gaagtgcacg ggaaggcgta
      121 aatccaggaa tactagctac atacggtgac gggggaatag ttaaaacgga cgaacccggt
      181 cataaggtgc cttcctcact acgagctatt agctctcgta atgtaaaacg gtttggtgaa
      241 attagtggaa atgtaggtac agaaactcgc cctcgcaacg ttgcatttaa ttacatcgta
      301 agagccgaat gaaaacgttg gtttggggga aggctctgcg ctgcccgttg gtgtgcctgt
      361 tccgtggccc tcagccactc cgccaactgg gtggctgaaa tgcaatggtg cagcattttc
      421 ttctgaaaag tacccaaatc tggcaaaggc ttaccctact aataaattgc cggatttacg
      481 cggtgaattt attcgtggct gggatgacgg acgtggtgtg gatgccgggc gacaattatt
      541 atcttcacag ggggatgcaa taagaaatat tgaggggttc gcagatggcg ggattggcat
      601 gtcttttgat gcaatcagag gggcttttta tgatgcagga acacgatctg cgagaatgcc
      661 gaataacaca actgatatag gcaaaaccga tgaccttgga ttcgacgcct ctcgtgtcgt
      721 gccaacagct aatgaaaacc gtcctcgtaa tattgccttt aattatatcg taagggcggc
      781 ataaaaacgt tggtttgggg gaaggctctg cactgcccgt tggtgtgccc gttccatggc
      841 cctcagccac gccgccaacg ggatggctga aatgtaacgg agcagcattt tcttctgaaa
      901 agtacccaaa tctggcaaag gtttacccca ctaataaatt gccggattta cgcggtgagt
      961 ttatccgtgg ctgggatgac ggacggggcg ttgataatgg acgcaaccta ctgtctgcgc
     1021 agtctgacgc tattcagaat atagttggca cttttgggcg tactcagctt tttaaagatg
     1081 cgctgaattc agggccattt agtcaaactg actctatatt atcagtaggc ttacaaccaa
     1141 ctgagatcct tgaaggatat ggtgcttctg tatggacatt cgacgcctct cgctcagttc
     1201 gcacagcgtc tgaaacgcgc ccccataaca ttgcgtttaa ttacatcgta agggcggcat
     1261 aaaaacgttg gttt
//
LOCUS       DBBGLQ010000364         1270 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.370, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000364 DBBGLQ010000000
VERSION     DBBGLQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.370"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..226)
                     /locus_tag="ACIYSB_005722"
     CDS             complement(<1..226)
                     /locus_tag="ACIYSB_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432493.1"
                     /translation="MQQISFLPGEMTPGEHSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGET"
     gene            complement(241..726)
                     /locus_tag="ACIYSB_005723"
     CDS             complement(241..726)
                     /locus_tag="ACIYSB_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGC5432494.1"
                     /translation="MTTFSHSSTTPSVSVTTASGNNQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFIAPEPDEDDGETWILFNA
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(818..>1270)
                     /locus_tag="ACIYSB_005724"
     CDS             complement(818..>1270)
                     /locus_tag="ACIYSB_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGC5432495.1"
                     /translation="CTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKR
                     DAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTTDILTPRRREDYGKDLWSAYQTIQ
                     ENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT          274 a          357 c          354 g          285 t
ORIGIN      
        1 gggtttcacc ggcaatcagc tgattctggt tgttcagata cagcacccgg aactcttcac
       61 gctccagtcc cgccatgttc agaatcagcc attcccgtgc cgcacgggtg gaggtgaagg
      121 ccacgccggg ttcatgaaga tggcggtcca gggttttcag ggcccgctga atgaggctgt
      181 gctcaccggg cgtcatttct ccgggcagaa aggaaatctg ttgcattgtt ccggcccctt
      241 tcagtcaatg atgcgcataa tggcactgca ttccggatgt tgcagggcgt aatcccgcag
      301 ccggtaatag tggaccgtca tggcataaca ctccgtacga caggcatgat ggctgtacgc
      361 catcagacag gcggcaatgc cggcggcttc cgggctcatt tcagcgcggt taccgttcat
      421 ggcattgaac agtatccagg tttctccatc atcttcatcc ggttcaggtg ctataaatgc
      481 gccaccgttg ttcagggtgt acagattcca gatacccccg cagtagtctt cacacagacg
      541 gtccatccag ccgaagacac ggggctccag ggtcacccac tgtggaatga ggccaaagtg
      601 ctgcggccag aagctgatgc gctgttcatc agggacggga gtggcaacca gttgagactg
      661 gttattccct gatgcagtag ttacggaaac agaaggtgtg gtggagctat gcgaaaaggt
      721 tgtcatgaga tgattcctta taaaaagtaa atgaatggaa gaatgcctgg ggactggtac
      781 agacgtgagt cagggctgca ctttcaggga aacagcatca gcgcatgctc tccagcagcg
      841 tttctgccat cacccacaac gcccggttga gcttaatgtc ggtatcgatg ctgtgaatgg
      901 cccgggtatg gatacgtttt cctctggcac tgcgaccgga aatcccgcct ttcagcatat
      961 tttcctggat ggtctgataa gcactccaca ggtccttacc gtaatcctcc cggcgtcgtg
     1021 gcgtcagaat gtcggtagtg gtgacgggct gatgttcatc accataacgg taagtcagcg
     1081 ccgcctgtgc cagcgcctgg cgtgccggtg gcggcaggac cagcgactgc atggcatcac
     1141 gcttttcctc aatccggtca aacacgccca ccacttcgta agcaccttca atcaccctgt
     1201 ccaccacgtt tccccggtgg ggcacccgga cttctcccag cgactgaccg cagaccaagc
     1261 cattggtgca
//
LOCUS       DBBGLQ010000365         1263 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.371, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000365 DBBGLQ010000000
VERSION     DBBGLQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.371"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..328)
                     /locus_tag="ACIYSB_005725"
     CDS             complement(<1..328)
                     /locus_tag="ACIYSB_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432496.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPGGKCIITGDFQKELAYLQNVILYNVSSLRLDFLGYNAQIIQRSD
                     NTCELTI"
     gene            complement(350..889)
                     /gene="yhaB"
                     /locus_tag="ACIYSB_005726"
     CDS             complement(350..889)
                     /gene="yhaB"
                     /locus_tag="ACIYSB_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HGC5432497.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYHGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1145..>1263)
                     /locus_tag="ACIYSB_005727"
     CDS             complement(1145..>1263)
                     /locus_tag="ACIYSB_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HGC5432498.1"
                     /translation="FYQYFKISNTCIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          396 a          219 c          234 g          414 t
ORIGIN      
        1 taatggtaag ttcacaagta ttgtccgatc gttgaataat ttgggcgtta taacctaaaa
       61 aatccagacg aagagacgag acattataaa gaattacatt ttgtaagtac gctaattctt
      121 tttgaaaatc accagtgata atgcattttc caccaggttg taacgtgtta agtaaagtga
      181 ccatttcaat agaggtctgt tttagttttt ctatacctgg tgctagtgct tcgaggttat
      241 ttcctgttgg gctttcagga aggtcattca tttttcgacg gactaaatca ctactaatat
      301 cattaccaat tgatgaaaca gggaacatta ttttatcctt tataaataat caataaaaat
      361 cattattaaa atgatcgggt gaaaaaaaga gaatttgcca acaatctgaa gtaatgctgg
      421 ttttattaaa gtgcacaagg ggggttaaga tggcttcttt tcgggtgaat tttgcacaat
      481 acttagaaat cgtctcctta atacattcca gctctttttt attgacggct gagcaagtac
      541 attgttcacc aaaaggtgca aaagtgccta catctaatgt ttgtatgata taatcatcat
      601 gttctcctgt agaaatatct ataatgaatg gactcatatt ttccggttgg attcggatca
      661 tgacagaagg aacatcgcca tgatagaaat tcaatgcagt tattgcagat aatactctcc
      721 gcagttcatt acttatactg taaattttat taacgtcatt ggtggagaca atttctgcaa
      781 actttctttt taccgtccaa taatcaatat ttctattgtg attgttaacc actgcgtgca
      841 ttggcgggat tgaagggtga aaaatatgcg caattgggaa gcctttcatt gatgcctgcc
      901 ttgtcatcat gacaaacaat aatagatgct tattttcctc gcagggttct ttcgcactat
      961 caaaaaacgc tcatttaccc ggcgttacag taaacgaagc aacattctgt ggcctacgcc
     1021 gatcaaaagc tctgtgaaaa tttcccttgt tgtctttgtc tgaccttatc gcggcgatca
     1081 gcaattattc gttttgtgat cctcaacaca aaatgctatt cgtcgcctat gagtggagaa
     1141 gaatttatgg aaaacgattg cattcacaaa atgacgtcct gtcagaagag aaaaagttga
     1201 cgttaatcaa ttgttcgaga tgatcaatac atgtattcga aattttaaaa tattgataaa
     1261 ata
//
LOCUS       DBBGLQ010000366         1263 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.372, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000366 DBBGLQ010000000
VERSION     DBBGLQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.372"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(81..812)
                     /gene="rlmB"
                     /locus_tag="ACIYSB_005728"
     CDS             complement(81..812)
                     /gene="rlmB"
                     /locus_tag="ACIYSB_005728"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGC5432499.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(992..>1263)
                     /locus_tag="ACIYSB_005729"
     CDS             complement(992..>1263)
                     /locus_tag="ACIYSB_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463229.1"
                     /note="3'-5'exoribonuclease that acts nonspecifically on
                     poly(A), poly(U) and ribosomal RNAs; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGC5432500.1"
                     /translation="ISSERAPRNVGKTAREKAKKGDAGKKGGKRRQVGKKVNFEPDSA
                     FRGEKKTKPKAAKKDARKAKKPSAKTQKIAAATKAKRAAKKKVAE"
BASE COUNT          256 a          358 c          302 g          347 t
ORIGIN      
        1 ttgcaatacg taattatctt accagctata gacaaaaaaa aaccatccaa atctggatgg
       61 cttttcataa ttctgagaaa ttagctgcgc tggcgcaccg cttcaaataa gcaaattccg
      121 gtcgcaaccg aaacgttcag ggaagaaacg cttccagcca tcgggatgct gatcaactca
      181 tcgcaatgtt cacgagtcag gcgacgcata ccttcacctt ccgcacccat caccagcgcc
      241 aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc cgtaccgacg
      301 atccagatat tctcttcctg caacatacgc atggtgcgcg caaggttagt cacccgaatc
      361 agcggaacgc tttctgccgc gccgcaggct acttttttcg ccgtagcgtt gagctgtgcg
      421 gagcgatctt tcggcacaat caccgcatga acgcctgcgg cgtccgcact acgcaggcac
      481 gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca gcaggaacgg ttgatcgagc
      541 gaagcgatca gatccggcag atcgttttcc tgatactgac gtcctggctt cacgcgggcg
      601 ataatgccct gatgcacggc accgtcgctt ttctcgtcga gatattggcg gtttgccaac
      661 tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg tttatcttca
      721 cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag cagggcctgc
      781 actgcgtgga tgccgtaaat catttcgctc attaatgtac tcgttgttta cgttttgcct
      841 gatgcactac gtttatccgg cctacatgat ctctgcaata tattgaattt gcgtgattat
      901 gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcatt ttgtcagcaa
      961 tctaaccctc ttcttttaaa gagggtattg atcactctgc cacttttttc ttcgccgcac
     1021 gcttcgcttt ggtcgctgca gctattttct gcgttttcgc cgatggcttt ttcgcttttc
     1081 tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc accgcggaag gcgctgtctg
     1141 gctcaaagtt taccttttta ccgacctgac gacgcttgcc gcctttttta cctgcatcgc
     1201 cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt tcgctggaga
     1261 tca
//
LOCUS       DBBGLQ010000367         1261 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.373, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000367 DBBGLQ010000000
VERSION     DBBGLQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.373"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(280..420)
                     /locus_tag="ACIYSB_005730"
     CDS             complement(280..420)
                     /locus_tag="ACIYSB_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGC5432501.1"
                     /translation="MSQKSLTAITFCVTAILIIWILHGSLCEIRMSFWGAEFAAFLQC
                     KK"
BASE COUNT          318 a          278 c          290 g          375 t
ORIGIN      
        1 tgttgatgaa aaaaacaaag atttaattta ttgatataaa ggatttgtat cagcgtatgt
       61 ccacgcagtg accacatttt cgagaggtag agtaagcccg caacattgcg ggctttgtct
      121 tatagtctga tcactgtttc aagtcggtga ggacaccaaa aagaaaaccc gcagttttta
      181 tgctgcgggt ttgtttgttc atgtctgtga gatagggtgc cttatcgacc ttaccctggc
      241 aaccgattga cgggggattg ctcccccgtc gcggtttcct tacttcttac attgtaagaa
      301 cgccgcaaac tccgctcccc agaagctcat ccgtatttcg cacagcgaac cgtgcaatat
      361 ccagatgatg aggattgccg tcacgcagaa cgtgatggcc gtaagcgatt tttgcgacat
      421 agcacttgct ccttttccgg agaggcgcta acctttcact tgtcaaggta atgcggttag
      481 ggcctcggtt aaacagaaat gttttccggg gcctttccac atccggcctt caggttttcc
      541 ctccggccat cagccgaaag gcacccgcgc gtaatttaac tgtttctttt tgttgcggca
      601 atatttcagg tgtcagttgg ggtgggctgt atcttgtctg atgcataaaa aaatgcccgc
      661 gcttcggcgg gcaaaaaaca agacttttcg tttgtatatc atggataatg gacacccttc
      721 ctgttgtcag ggggaggtac ccgtagctaa tctactcact ttgtgcccgc agctcaaccc
      781 tgaagttttc atggaaaatc agggctgctt tgtgtgcgcc agggaaggcg cctcgatggc
      841 aggcgcaaaa aaaatgcccg ctctccggcg ggcaaaacaa tgatctacgt cacattatat
      901 tgataaccgg gcattcctcc atgcccattt ttaatttagc catttttcat tttgttcaca
      961 atatagttat ctatcatatt catgaatgaa attataattt catctatagg tataaatgat
     1021 ttgtgttaat atgtttctcc tgtctaactg ttttctcatt tttcgatact agtatttctg
     1081 atatattcag ggggcgtatg agattgttcc gaagaaacga tattgagatt gaactgcatt
     1141 tctcctccgg tgaggataag gttaaatcaa taaaggtgaa actaagacat gcccgggatg
     1201 ttcaggcgtg tcagtgtctt attgatgaac tcgttgctgc cggaggaacg ccaaaaaaac
     1261 a
//
LOCUS       DBBGLQ010000368         1248 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.375, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000368 DBBGLQ010000000
VERSION     DBBGLQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.375"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            16..1180
                     /locus_tag="ACIYSB_005731"
                     /pseudo
     CDS             16..1180
                     /locus_tag="ACIYSB_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          349 a          292 c          315 g          292 t
ORIGIN      
        1 agtttagtct ccaggatttc cggggcggtt cagtccggcc aagcattaac cggcaaggat
       61 ggtatcctaa cgccattaat caaacagtta acagaaaccg ctctggccgc tgaactcgac
      121 tcccatctgg ctcaggattt tgaagtcaat cgtaaaaatg gttccggaaa aaagaccatt
      181 aaagccccga ctggcagctt tgagctggca actccacgtg atcgtaatgg ttattttgaa
      241 ccccaactgg tgaagaagca ccagaccacg ctgtctgatg agctcgagcg caagatcatt
      301 cgcctgttcg cgctggggat gagctaccag gatatcagcc gggagatcga agatttatat
      361 gccttcagtg tttccacggc taccatcagt gcggttaccg ataaagtcat tcctgagtta
      421 aaacagtggc agcagcgccc gctggaaaag gtttatccct tcgtctggct ggacgccatt
      481 cactataaaa tccgcgaaga tgggtgttat cagagcaagg cagtttatac cttactggcg
      541 ctgaatctgg aaggcaaaaa agaagtcctg ggcctgtatt tgtcggaaag cgaaggcgct
      601 aacttctggc tgtcagtatt aagtgatctg caaaaccgtg gcgttgaaga cattctgatt
      661 gcctgcgtgg atgggctgac cggcttccct gaagcgataa acagcattta tccgcagaca
      721 gaggtacagt tgtgcgtcat tcaccagata cgtaactcga ttaaatatgt ggcgtcaaaa
      781 caccataaag cgttcatggc cgacctgaag ccagtgtatc gtgcggtgtc ggaaacggcg
      841 ctggatgagc tggaagcgaa acgggggcag caatatccgg tggtgctcca gtcctggcgc
      901 cgcaagaggg aaaatctgtc agcgtacttc cgctatccgg cgaatatccg caaggtgatc
      961 tacacaacca acgcgataga atcagtccac cgccagttca ggaaactgac caaaacctaa
     1021 aggtgctttc ccgaatgaaa acagcctgtt gaagctactt tatctgggac tgatgaacgc
     1081 acaggagaaa tggacaatgc ccatccaaag ctggaatttg acattgtcgc agttagccat
     1141 ttattttgaa gggcggctga ataatgtgat gacgctgtag aatttttaac gtgacacaga
     1201 attctgaacg ctctcaaatt aaaatcatcg atatttaaca agtttaaa
//
LOCUS       DBBGLQ010000369         1246 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.376, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000369 DBBGLQ010000000
VERSION     DBBGLQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.376"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            234..>1246
                     /locus_tag="ACIYSB_005732"
     CDS             234..>1246
                     /locus_tag="ACIYSB_005732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HGC5432502.1"
                     /translation="MGRPLKTLFKREHSEEISNPSDNRAFSQVAEVYLSRRRLLQAGT
                     VAGAAAAFPFLLKQENALAKVVSKASGNLGKATTLGFTSLPVSTEDTVIVPEGYIAKP
                     FYKWGDATGIAGNLPVFKTDGSNTTEEQAAQAGMHHDGMAWFSLPQGGNSSDHGLLAI
                     NHEYIDNGLLFKDGDANWSADKALKGQNAMGVSVIEVKKVPLGWEVVRPSSFARRITV
                     NTPMKITGPALHNPLMQTVDDPKGEIILGTMQNCANGFTPWGTYLTCEENWSDIFVKK
                     AEMNPLEKRYGISGSDDSYRWNEVDKRFSVDATPNEPNRFGWVVEIDPYDPHSVPRKH
                     TALG"
BASE COUNT          331 a          336 c          330 g          249 t
ORIGIN      
        1 tcgcaaaccg catcgaacca gtacttgcct cacccgggac gtccatatat gctcaacaaa
       61 agagagcgtt cagaattcta gcctcacatc atacctgaaa ccgcgtcccg accatagatg
      121 attttatcgc cggtttttta tgacaattgt tatatttccg taacaatctt tccgtagact
      181 cacgtgcata aaaatgattc cccttgaaat cgattttttg aaaggaaacc gcaatgggca
      241 gaccgttaaa aactttattc aaacgtgagc atagtgaaga aatcagtaac ccgagcgata
      301 accgcgcgtt ttcgcaggtg gcggaagtct atttgtcacg ccgccgtctg ttacaggcag
      361 gcacggtcgc cggtgccgcg gcagcgttcc catttctgct gaaacaggaa aacgcgctgg
      421 cgaaagtggt ctccaaagca agcggtaacc tcggcaaagc gacgacgctc ggtttcacca
      481 gcctgccggt ttccacggaa gataccgtta tcgtgccgga aggatacatc gcgaagccct
      541 tctacaaatg gggcgacgcc accgggattg ccggaaatct gccggttttt aaaaccgacg
      601 gtagcaacac cacggaagaa caggcggcgc aggccgggat gcaccacgac ggcatggcgt
      661 ggtttagcct gccgcagggc gggaacagct ccgatcacgg cctgcttgcc ataaaccatg
      721 aatacatcga taacggtcta ctgtttaaag atggcgatgc caactggagc gccgataaag
      781 ctcttaaagg ccagaacgcg atgggcgtgt cggtcattga agtaaaaaaa gtgcccctgg
      841 gctgggaagt ggtgcgcccg tcatcgttcg cacgccgcat caccgttaac acgccgatga
      901 aaatcaccgg tccggcgctg cataacccac tgatgcaaac agttgacgat ccgaaaggcg
      961 aaatcattct cggcaccatg cagaactgtg ccaacggatt tacgccgtgg ggcacctatc
     1021 tgacgtgtga agagaactgg tccgatattt tcgtaaaaaa agcagagatg aacccgctgg
     1081 aaaaacgtta cggtatcagc gggagcgacg actcttaccg ctggaacgaa gtggataaaa
     1141 ggttcagcgt cgacgccacg ccgaacgagc cgaaccgctt cggctgggtg gtggaaatcg
     1201 atccatacga tccgcattca gtgccgcgca agcacaccgc gcttgg
//
LOCUS       DBBGLQ010000370         1241 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.377, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000370 DBBGLQ010000000
VERSION     DBBGLQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.377"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>1241
                     /locus_tag="ACIYSB_005733"
     rRNA            <1..>1241
                     /locus_tag="ACIYSB_005733"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          312 a          287 c          392 g          250 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacgga agttttcaga
      721 gatgagaatg tgccttcggg aaccgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttggatca c
//
LOCUS       DBBGLQ010000371         1233 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.378, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000371 DBBGLQ010000000
VERSION     DBBGLQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.378"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            77..1213
                     /locus_tag="ACIYSB_005734"
                     /pseudo
     CDS             77..1213
                     /locus_tag="ACIYSB_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          403 a          222 c          277 g          331 t
ORIGIN      
        1 atattcctta ttacctaaag ctgtttttca tagcttatac atgatcaaat actccttaca
       61 taaataaggt gaacaaatgg aacttaaaaa attgatggaa catatttcta ttattcccga
      121 ttacagataa gcctggaaag tagagcataa attgtcaggc atcctactat tgactatttt
      181 tgccgttatt tctggtgcag aaagttggga agatatagag gatttcgggg aaacacatct
      241 cgatttcttg aagcaatatg gtgattttga aaatggtatt cctgttcacg atactattgc
      301 cagagttgta tcctgtatca gtcctgcaaa atttcacgag tgctttatta actggatgcg
      361 tgattgccat tcatcaaatg ataaagacgt cattgcaatt gatggaaaaa cgctccggca
      421 ctcttatgac aagagtcgcc gcaggggagc gattcatgtc attagtgcgt tctcaacaat
      481 gcacagtctg gtcatcgggc agatcaagac ggataagaaa tccaatgaga tcacagctat
      541 ccctgaactt cttaacatgc tggatattaa aggaaaaatc atcacaactg atgcgatggg
      601 ttgccagaaa gatgttgcag agaagataca aaaacaggga ggtgattatt tattcgctgt
      661 aaaaggaaac caggggcggc ttaataaagc ctttgaggaa aaatttccgc tgaaagaatt
      721 aaataatcca gagcatgaca gttacgcaat gagtgaaaag agtcacggca gagaagaaat
      781 ccgtcttcat attgtttgcg atgtccctga tgaacttatt gatttcacgt ttgaatggaa
      841 aggactgaag aaattatgcg tggcagtctc ctttcggtca ataatagcag aacaaaagaa
      901 agagccagaa atgacggtca gatattatat cagttctgct gatttaaccg cagaaaagtt
      961 cgccacagca atccgaaacc actggcacgt ggagaataag ctgcactggc gtctggacgt
     1021 ggtaatgaat gaagacgact gcaaaataag aagaggaaac gccgcagaat tattttcagg
     1081 gatacggcac atcgctatta atattttaac gaatgataag gtattcaagg cagggttaag
     1141 acgtaagatg cgaaaagcag ccatggatag aaactatctc gcgtcagtcc ttgcggggag
     1201 cgggctttcg taatctttcc ctgctttttg tac
//
LOCUS       DBBGLQ010000372         1230 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.379, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000372 DBBGLQ010000000
VERSION     DBBGLQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.379"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            87..362
                     /locus_tag="ACIYSB_005735"
     CDS             87..362
                     /locus_tag="ACIYSB_005735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432503.1"
                     /translation="MAKVSTRTVTNRLRALGYSLIKCGELHKNAVYSDCIVEIINQLR
                     DEQGLTFREIAQRLSHELQPNPTPALARHLYWRRTAADAVLRELLPD"
     gene            683..901
                     /locus_tag="ACIYSB_005736"
     CDS             683..901
                     /locus_tag="ACIYSB_005736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HGC5432504.1"
                     /translation="MANRKQRRATAEHHHVRAQIDKRLFRASRVARIMHINMLHERRG
                     ALPNTYIASVFSYLADDLAELQRLINQH"
     gene            974..>1230
                     /locus_tag="ACIYSB_005737"
     CDS             974..>1230
                     /locus_tag="ACIYSB_005737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432505.1"
                     /translation="MILADDFLEYLNNTERDLYARVLTRYEKYKLSLPEHQLREGDID
                     IEGRYIVRRRIAGYGIWRVTGREHSLLTICQSPSGVITEIVA"
BASE COUNT          301 a          388 c          309 g          232 t
ORIGIN      
        1 gcatcaagca gcaaagacta agcgatgaga tgatcgccac gctcatcagg aaccgccaca
       61 ccaggtccct caaggaactg gcacacatgg cgaaagtttc gacacgcacg gtaaccaacc
      121 gattgagggc gctgggctac agcctgatca aatgcggaga actgcataaa aacgccgtct
      181 acagcgactg catcgttgag attatcaacc agttacgcga cgagcagggc ctgacgttca
      241 gggaaatcgc gcagcgcctt agccacgaac tacagccgaa ccccaccccg gcacttgctc
      301 gccatctcta ctggcgccgc acagcagccg acgccgtact ccgcgaactg ttaccagatt
      361 gagcaatcac catgtacaca caaaaaaacc gcttgcaaca acaaaccgga acagggtacg
      421 cttcccccgc acctcataaa acgggtgtcg ggtttcgcag cctgctgact agcaaagcgc
      481 acaaccgcgc cagcggtttt tttgtgcgta ctgtattgcc acgtcttttt cgcacacgaa
      541 ttatggcggg gcgtacgggg ccgacttcgg tcgggccggg ttctttgcta gccggtactg
      601 cgaacctcgt acgtctcgcc acccacagtt tcgcagctct ggatggtgag tttttaaaac
      661 ttactagcaa agaggccaca ccatggcaaa ccgcaaacag cgccgcgcta ctgcggagca
      721 tcaccacgtc cgcgcccaaa tcgacaaacg ccttttccgc gcatcacgcg ttgcacgcat
      781 catgcacatc aacatgctgc atgaacgcag aggggcacta ccgaacacct atatcgcctc
      841 cgttttcagc tatctggcgg acgatctcgc cgagctgcaa cgcctcatta accagcactg
      901 ataaacatcc cgaagccatc ctgcgcccgt tcgcgggagg cttccacaca tctgtagtcg
      961 ggaggattgc gccatgattc tggctgacga ttttctggag tacctgaaca acaccgagcg
     1021 cgacctgtac gcgcgcgttc tcacccgtta cgagaaatac aaactttcac tgccagagca
     1081 tcaactccgc gaaggcgata tcgacattga agggcgctac atcgtcaggc gccgcatagc
     1141 gggctacggc atctggcgtg tcaccggacg tgagcattca ttgctgacca tctgccagag
     1201 tccgtccggc gtgataacgg agatcgtcgc
//
LOCUS       DBBGLQ010000373         1227 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.380, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000373 DBBGLQ010000000
VERSION     DBBGLQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.380"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..302)
                     /locus_tag="ACIYSB_005738"
     CDS             complement(<1..302)
                     /locus_tag="ACIYSB_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGC5432506.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAA"
     gene            complement(318..803)
                     /locus_tag="ACIYSB_005739"
     CDS             complement(318..803)
                     /locus_tag="ACIYSB_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGC5432507.1"
                     /translation="MTSSSHNSTTPSVSVTAAPENNQPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMVPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(895..>1227)
                     /locus_tag="ACIYSB_005740"
     CDS             complement(895..>1227)
                     /locus_tag="ACIYSB_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGC5432508.1"
                     /translation="EEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTP
                     RRREDYGKDLWSAYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMA
                     ETLLENMR"
BASE COUNT          255 a          325 c          364 g          283 t
ORIGIN      
        1 gcggcattgt ggtacagggc acgtttaatt acctcccggg gatggacttc cgtgcggttg
       61 atggtgcctg tgaagagggt ttcaccggca atcagctgat tctggttgtt cagatacagt
      121 acccggaact cttcccgctc cagtcccgcc atgttcagaa tcagccattc ccgtgccgca
      181 tgcgtggagg tgaaggccac accgggttca tgaagatggc ggtccagggt tttcagggcc
      241 cgcagaatga ggctgcgctc gccgggcgtc atctctccgg gcagaaagga aagctgttgc
      301 attgtgcttc tctccattca gtcgatgatg cgcataatgg cgctgcattc cggatgctgc
      361 agggcgtaat cccgcagccg gtaatagtgg accgtcatgg cgtaacactc cgtacgacag
      421 gcgtgatgac tgtacgccat cagacaggcg gcaatgccgg cggcttccgg gctcatttca
      481 gcgcggttgc cgttcatggc attgaacagt acccatgttt cgtcatcatc gtcatccggt
      541 tcgggtacca taaatgcccc gccgttgttc agggtgtaca gattccagat accaccgcag
      601 tagtcttcgc acagacggtc catccagccg aagacacggg gctccagggt cacccactgt
      661 ggaatgaggc caaaatgctg cggccagaag ctgatgcgct gttcatcagg gacgggagtg
      721 gcaaccagct gaggctggtt attctctggt gcagcagtca cggaaacaga aggtgtggtg
      781 gaattatgcg aagaggatgt catgagatta ttccttataa aaagcaaatg aatggaagaa
      841 tgcctgggga ctggtacaga cgtgagtcag gacggcgctt tcaggtaaac ggtatcagcg
      901 catgttctcc agcagcgttt ccgccatcac ccataacgcg cgattgagct taatatcggt
      961 gtcgatgctg tgaatggccc gggtatggat acgttttcct ctggcactgc gaccggaaat
     1021 tccgcctttc agcatattct cctggatggt ctgatatgcg ctccacaggt ccttaccgta
     1081 atcctcccgg cgtcgtggtg tcagaatgtc ggcagtggtg acgggctgat gttcgtcacc
     1141 ataacggtaa gtcagtgccg cctgagccag tgcctgacgt gccggtggcg gcaggaccag
     1201 cgactgcatg gcatcacgct tttcctc
//
LOCUS       DBBGLQ010000374         1220 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.381, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000374 DBBGLQ010000000
VERSION     DBBGLQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.381"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>1220
                     /locus_tag="ACIYSB_005741"
     CDS             <1..>1220
                     /locus_tag="ACIYSB_005741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGC5432509.1"
                     /translation="SALVKAGVSGEAQIASISQSVARFSSASGVEVDKVAEAFGKLTT
                     DPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAATKGFDDQTRRLKEN
                     MGTLETWADRTARAFKSMWDSVLDIGRPDTAREMLEKAEKAFDEADKKWQWYQSRSHR
                     RGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKDWADSEASRLKYTEE
                     AQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQPG
                     VKVSAGERQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQFAVLEEAA
                     QRRQLSAQEKSLLAHKDETLEYKRLLAALGDKVTYQERLNALAQQADKFAQQQRAKRA
                     AIDAKSRGLTDRQA"
BASE COUNT          313 a          304 c          417 g          186 t
ORIGIN      
        1 agcgcactgg ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt
       61 gtggcgcgtt tctcctctgc atccggcgtg gaggtggaca aggtcgctga agccttcggg
      121 aagctgacca cagacccgac gtcggggctg acggcgatgg cgcgccagtt ccataacgtg
      181 acggcggagc agattgcgta tgttgctcag ttgcagcgtt ccggcgatga ggcaggggca
      241 ttgcaggcgg cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa
      301 gagaacatgg gcacgctgga gacctgggca gacaggacag cgcgggcatt caaatccatg
      361 tgggattcgg tgctggatat tggtcgcccg gacactgccc gggaaatgct ggagaaagca
      421 gaaaaggctt ttgatgaggc ggacaaaaaa tggcagtggt atcagagccg gagccaccgg
      481 cgcggtaaaa cctcagcatt tcttgccaat ctccggggag catgggagga cagagcgaat
      541 gcgcaacttg ggctttcagc cgccacgttg caggccgatc ttgaaaaggc cagagagatg
      601 gcagcaaagg actgggccga ctctgaggca tcacggctga aatataccga agaggcgcag
      661 aaggcttacg aacgcctgca gacaccgctg gagaaatata ccgcccgtca ggaagaactg
      721 aacaaggcac tgaaagacgg gaaaatcctg caggcagatt acaacacgct gatggcggcg
      781 gcgaaaaagg attatgaagc gacgctgaaa aagccgaaac agcccggcgt gaaggtgtct
      841 gcgggagagc gtcaggaaga cagtgctcat gctgccctgc tgacgcttca ggctgaactc
      901 cggacgctgg agaagcatgc cggagcaaat gagaaaatca gccagcagcg ccgggatttg
      961 tggaaggcgg agagtcagtt cgcggtactg gaggaggcgg cgcaacgtcg ccagctgtct
     1021 gcacaggaga aatccctgct ggcgcataaa gatgagacgc tggagtacaa acgcctgctg
     1081 gctgcacttg gcgacaaggt tacgtatcag gagcgcctga acgcgctggc gcagcaggcg
     1141 gataaattcg cacagcagca acgggcaaaa cgggccgcca ttgatgcgaa aagccggggg
     1201 ctgactgacc ggcaggcaga
//
LOCUS       DBBGLQ010000375         1204 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.382, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000375 DBBGLQ010000000
VERSION     DBBGLQ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.382"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..750
                     /locus_tag="ACIYSB_005742"
     CDS             <1..750
                     /locus_tag="ACIYSB_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432510.1"
                     /translation="YQRDIHPRKVNVPILQLEVAWINNNCRSSDSSTDIIILPRMIRR
                     MVVFNYTKINKQLEKVLGGIARKQTSERNKGGLYTRIKKESDFLSMDRFMVEIENIIR
                     PERFPTSIYPVFSHSFIERELPLLNLARSQYKRAISKRVANSIFYTLQKTNVALWLYL
                     EMFALVAFIKLYTGKGFEFIKNMCVFIDYEKGIIKSTHEELLGEIKRNIQVNLKVNPD
                     NFPELIDIYNSCFSENILSYDIQDGNFKWRS"
BASE COUNT          432 a          172 c          224 g          376 t
ORIGIN      
        1 tatcagagag atattcaccc cagaaaagtt aatgttccga tactgcaact tgaagttgca
       61 tggataaata ataattgccg ctcaagtgat tcaagcactg atattattat ccttccgagg
      121 atgataagaa gaatggttgt atttaattat acgaagataa ataaacaatt agagaaagtg
      181 ttggggggga tagcacgaaa acaaacctca gaaaggaata aaggcggtct ctataccaga
      241 ataaaaaaag aaagtgactt tctttctatg gatagattta tggtggaaat agaaaatatc
      301 ataagacctg aaaggtttcc tacatcaatt tatccagtct tttcacattc atttattgaa
      361 cgagagcttc cgctactgaa tcttgcccga tcacagtata aaagagcaat aagtaaaaga
      421 gtggcgaatt caatttttta tactttacaa aaaactaacg tggcattatg gctatatctt
      481 gaaatgtttg ctctggtggc attcattaaa ctgtatacag gaaaaggttt tgaattcata
      541 aagaacatgt gtgtatttat cgattatgag aaagggataa taaaatccac acatgaagaa
      601 ctgctgggtg aaattaagcg taacattcaa gtgaatttga aggtgaatcc tgataatttt
      661 ccagagttaa tagacatata caattcatgc tttagtgaaa atatattgtc atatgatatt
      721 caggatggta attttaaatg gcgctcatga tgtaaaaata ttaatttcac attacatatt
      781 aattatcaac aaaaaacata taattaattt caatgggtta catataaaaa tgttgatgat
      841 tagccagctt agtatggtta ttcaatgtga tgcttaacgc ttacagataa taacacattc
      901 atatgagcta tatatcataa tattcaataa tcatgatatg ctaatctgat tatggataaa
      961 aattactgaa tgacgccata ttatgattgg cagtgtttcc tgtatacgat gacttatctc
     1021 tcccgcatca tatgggggca cgtttcttag gaaaagtgac ggggttacag aagccaatta
     1081 cgatagtttg ttgtagagtt atcacgagtt tttgttgttc tgaagacgga atcagaaaaa
     1141 taattccaga gtaaacctgt ctgttttttg accagtaaag atccatgctg gccgtgaaaa
     1201 actg
//
LOCUS       DBBGLQ010000376         1202 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.383, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000376 DBBGLQ010000000
VERSION     DBBGLQ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.383"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>1202
                     /locus_tag="ACIYSB_005743"
     CDS             <1..>1202
                     /locus_tag="ACIYSB_005743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGC5432511.1"
                     /translation="TALVNAGVRGGEQFEAISQSVARFSSASGVEVDKVAEAFGKLTT
                     DPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAATKGFDDQTRRLKEN
                     MGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKKADDIWNLRKDDYFV
                     NDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEASRLKYTEEAQKAYE
                     RLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQSGVKVSAG
                     DRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQFAVLEEAAQRRQLS
                     AQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFAQQQRAKRAAIDAKS
                     RGLTDRQAA"
BASE COUNT          313 a          290 c          408 g          191 t
ORIGIN      
        1 acggcgctgg tgaatgccgg tgtgcgtggt ggtgagcagt ttgaggcgat cagccagagt
       61 gtggcgcgtt tctcctctgc atccggcgtg gaggtggaca aggtcgctga agccttcggg
      121 aagctgacca cagacccgac gtcgggactg acagcgatgg cacgtcagtt ccataacgtg
      181 acggcggagc agattgcgta tgttgctcag ttgcagcgtt ccggagatga agccggggca
      241 ttgcaggcgg cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa
      301 gagaacatgg gcacgctgga gacctgggca gacaggacag cacgggcatt caaatccatg
      361 tgggatgcgg tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca
      421 gaggctgcgt ttaagaaagc ggacgacatc tggaatctgc gcaaggatga ttattttgtt
      481 aacgatgaag cgcgggcgcg ttactgggat gatcgtgaaa aggcccgtct tgcgcttgaa
      541 gccgcccgaa agaaggctga gcagcagagt caacaggaca aaaatgcgca gcagcagagc
      601 gataccgaag catcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg
      661 cagacgccgc tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac
      721 gggaaaatcc tgcaggcgga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa
      781 gcgacgctga aaaagccgaa acagtccggc gtgaaggtgt ctgcgggcga tcgtcaggaa
      841 gacagtgctc atgctgccct gctgacgctt caggcagaac tccggacgct ggagaagcat
      901 gccggagcaa atgagaaaat cagccagcag cgccgggatt tgtggaaggc ggagagtcag
      961 ttcgcggtac tggaggaggc ggcgcaacgt cgccagctgt ctgcacagga gaaatccctg
     1021 ctggcgcata aagatgagac gctggagtac aaacgccagc tggctgcact tggcgacaag
     1081 gttacgtatc aggagcgcct gaacgcgctg gcgcagcagg cggataaatt cgcacagcag
     1141 caacgggcaa aacgggccgc cattgatgcg aaaagccggg gactgactga ccggcaggca
     1201 gc
//
LOCUS       DBBGLQ010000377         1198 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.384, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000377 DBBGLQ010000000
VERSION     DBBGLQ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.384"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..976
                     /locus_tag="ACIYSB_005744"
                     /pseudo
     CDS             1..976
                     /locus_tag="ACIYSB_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    44..160
                     /locus_tag="ACIYSB_005744"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          287 a          308 c          334 g          269 t
ORIGIN      
        1 atcgtgaact gcgccgcagt aacgatatcc ttcgccaggc ttccgcttat tttgcgaagg
       61 cggagttcga ccgcctctgg aaaaaatgat gccactgctg gataagctgc gtgagcagta
      121 cggggtcgga ccggtatgca gcgaactgca tattgccccg tcaacgtatt accactgtca
      181 gcaacagcga catcatccgg ataaacgcag tgcccgtgcg cagcgcgatg actggctgaa
      241 gaaagagata ctgcgcgtat acgatgaaaa tcatcaggtg tacggtgtgc gtaaagtctg
      301 gcgtcagttg ttacgggaag gtatcagggt ggccagatgc actgtggcac gtctcatggc
      361 ggttatagga cttgccggtg ttctccgggg taaaaaggtc cgtacgacca tcagccggaa
      421 agccgttgcc gcaggcgacc gcgtaaaccg tcagttcgtg gcagaacgtc ctgaccagct
      481 gtgggtagct gattttactt acgtcagcac atggcagggc ttcgtctatg tggcgttcat
      541 cattgatgta tttgccggat acatcgtggg gtggcgagtc tcatcgtcta tggaaacgac
      601 attcgtgctg gatgcactgg agcaggcgtt gtgggcccgc cggccgtccg gcacaatcca
      661 ccacagtgat aaaggttctc agtatgtatc gctggcctac acgcagcggc ttaaggaagc
      721 cggcttactg gcctcaacag gaagtactgg tgactcgtat gacaacgcga tggcggagag
      781 catcaatggt ctttacaaag cggaggtaat acaccgtaag agttggaaaa accggacaga
      841 agtggaactg gccacactaa cgtgggtgga ctggtataac aatcgacgat tgctggaaag
      901 gctgggccat attcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaatac
      961 tgatctggca gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcaac
     1021 attcgtccct gtctgtccaa cggggaaacc gaaaataccc cattgtgtaa ttaccccatc
     1081 cggcccctta ctccatccag atttaggatt cggccagtct gctgctccag agtgccccgt
     1141 tgtaaaacta ctcatatctg gtacttgccc ggtatccgtt ccgacgttcc tcgttgcc
//
LOCUS       DBBGLQ010000378         1196 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.385, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000378 DBBGLQ010000000
VERSION     DBBGLQ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.385"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            210..>1196
                     /locus_tag="ACIYSB_005745"
     CDS             210..>1196
                     /locus_tag="ACIYSB_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HGC5432512.1"
                     /translation="MELQTRLVFIDTSAFEKKNYQFGQHALGRLQELIEDEKIHLLIT
                     DVTRKEIDSHLKHKSEEAASMIKKMQRDAMFLRNTPDLDCHGIFTKIKGDDIYAVISE
                     KFDDFVENGYVETIDVSTVNPQVVFDAYFNNLPPFGKESKKHEFPDAFALEAIKQVSL
                     ARGYSAYIVSDDGDMKSFCEKEDNFIHLENVDDLIDLIVRSDKAYEEPAKFADEIFEQ
                     LLEQIKADALQALEDGEFNYENADPFDETINSIEINSVNVGKKNLQNVDAEWAEYEVE
                     FEVVVTADYRFSDYDRSPWDPEDKQYVFVLSNESTVKHKETYTAHITLAYTDG"
BASE COUNT          388 a          240 c          237 g          331 t
ORIGIN      
        1 cgtgctgaag ctggtctccg caaacttgtg atctaggcca acacgccttt gcccccaaaa
       61 catttaaagc tatccctcat cacaccttgc taactcgtta cttgcccgaa tttttgtaat
      121 gagccaatat gctattagtt ggttatccac ttaattgttt tatcaattta tttgatgaca
      181 atttacacca tgtgttcaag gtattgatta tggaattaca gacaaggcta gtttttatcg
      241 atacaagcgc ttttgaaaag aaaaactatc aatttgggca acacgctctt ggtcgtcttc
      301 aagaactaat tgaggatgag aaaatccact tgctcattac tgatgtgaca aggaaagaga
      361 tcgactccca tctcaaacac aaatctgaag aagcagcttc tatgataaag aagatgcaaa
      421 gagatgcgat gttcctaaga aacacccccg accttgattg tcatggcatc ttcaccaaga
      481 ttaaaggtga tgatatttat gctgttatca gtgaaaaatt tgatgatttc gtcgaaaacg
      541 gctacgtcga aacaattgat gtaagcactg ttaaccctca agttgtattt gatgcttact
      601 tcaacaacct tcctcctttt ggcaaagaat caaaaaagca cgagtttcct gatgcatttg
      661 cgctagaagc tattaaacag gtgagcttgg ctagaggata ttcagcctat atcgttagtg
      721 acgacggcga tatgaaatca ttctgtgaga aagaagataa cttcattcat cttgaaaatg
      781 tcgatgacct aattgacttg attgttcgaa gcgacaaagc gtacgaagaa ccagccaaat
      841 ttgctgatga aatattcgaa caactcctag aacaaatcaa agcggatgcc ctgcaagctc
      901 tcgaagatgg cgagttcaac tatgaaaacg cagatccttt cgatgagact atcaattcta
      961 tagaaatcaa ctcggtgaat gttgggaaga aaaacttaca gaatgtcgat gccgagtggg
     1021 ctgaatacga agtagaattt gaggtggttg ttacggcaga ttatcgattc tcagattatg
     1081 accgttcacc ttgggaccct gaagacaagc aatatgtatt cgtcttgagt aatgagtcaa
     1141 cagtaaaaca caaagaaaca tacactgccc acataacact tgcctatacc gatgga
//
LOCUS       DBBGLQ010000379         1194 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.386, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000379 DBBGLQ010000000
VERSION     DBBGLQ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.386"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            43..1134
                     /locus_tag="ACIYSB_005746"
     CDS             43..1134
                     /locus_tag="ACIYSB_005746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HGC5432513.1"
                     /translation="MKELSVTVPSPDIDITINNNTLANLTERLMTLTLTDNRAFEADR
                     VTLTLDDTDGQLQLPPRGARLRVMIGWRGESLVNKGTYVVDEVAWEGPPDKLIITASS
                     ADFREEFNVRREVSWHDVTVKQVVSAIAHRYGLKTQISEILMDIEIDHADQTEESDIS
                     FLTRMAEMLGAIATVKNGSLLFILPGGGTGPDGRALPSFAITRSSGDSHRFRIADRDA
                     YTGVRAHWLDLKFGQKKKVNVKRRREAGKQKQEKSSSREGDYMEGAEGNVYVLRRTYQ
                     NEETARRAAAAKWQELQRGAAAFSITLARGRADLYPEMHGRVTGFKSEVDNQDWIIAR
                     VEHIIDESGFTTQLELEAKIPEWIADSGG"
BASE COUNT          328 a          312 c          348 g          206 t
ORIGIN      
        1 cggtgcatga aaccattaca gaacgcacca ggagagaaca ccatgaaaga gctgtcagtt
       61 accgttccgt caccggatat cgacatcacc atcaacaaca acacactggc aaacctcaca
      121 gagcgactca tgacgctgac cctgacggat aaccgggcct ttgaggcaga ccgcgtcacc
      181 ctcacccttg acgatacgga cgggcagtta cagttgccac cacgtggcgc acgcctgaga
      241 gtgatgattg gctggcgagg agaatcactg gttaacaagg gcacctacgt cgttgatgag
      301 gttgcgtggg aaggacctcc agataaactg atcatcaccg ccagcagcgc agattttcgg
      361 gaagagttca acgtgaggcg ggaggtatcc tggcacgacg tgaccgtaaa acaggttgtg
      421 tcagccatcg ctcaccgcta cggcctgaag acacagataa gcgaaatcct catggatatc
      481 gaaattgacc acgctgacca gaccgaagag agcgatatat ccttcctgac ccgcatggct
      541 gaaatgctgg gtgcgattgc caccgtaaaa aacggcagcc tgttattcat tctgccgggt
      601 ggcggaaccg ggccggacgg aagagccctg ccttcgttcg ccattacccg cagcagcggc
      661 gacagtcacc gctttcgcat tgctgaccgt gatgcgtata cgggggtccg ggcgcactgg
      721 ctcgatctga agttcgggca aaagaaaaaa gtgaacgtga agcgacgcag ggaagccggg
      781 aaacagaaac aggagaagag cagcagccgg gagggtgatt acatggaagg cgcggaaggg
      841 aacgtctacg ttctgcgcag gacttaccag aatgaagaaa ccgcacgacg tgcagctgcg
      901 gcaaagtggc aggagttaca gcggggagca gcggcattct ccatcactct ggcacggggt
      961 cgtgccgacc tgtaccccga aatgcacggc agggtaacag gttttaaaag cgaggtggac
     1021 aatcaggact ggattattgc cagggtggag cacatcatag atgaaagcgg cttcaccaca
     1081 caactggagc tggaagcgaa aatccccgaa tggatagcag attcaggcgg ataaaattaa
     1141 aatgagccat ctccactcag ccagataagg agaagccatg ttccgttgtc ccct
//
LOCUS       DBBGLQ010000380         1187 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.387, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000380 DBBGLQ010000000
VERSION     DBBGLQ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.387"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            43..>1187
                     /locus_tag="ACIYSB_005747"
     CDS             join(43..280,280..>1187)
                     /locus_tag="ACIYSB_005747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGC5432514.1"
                     /translation="MSGKRYPEEFKIEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSANKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNT
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     KRWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPQ
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSDQMSPTEYENQYYQRLGSV"
BASE COUNT          310 a          289 c          327 g          261 t
ORIGIN      
        1 gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat
       61 cccgaagagt ttaaaattga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc
      121 agcgttgcaa cacgtctcga tatcaccacc cacagccttt acgcctggat aaagaagtac
      181 ggtccggatt cttccgctaa taaagaacag tcagatgctc aggccgagat ccgccgtctc
      241 cagaaagagc tgaagcgggt taccgacgaa cgggacatat taaaaaaagc cgcggcgtac
      301 ttcgcaaagc tgtccgactg aggtacgcct ttatccgtga caacacctgt tgctggcctg
      361 ttcgcctgct ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc tggcttcagc
      421 agccgcattc acaacgccat caggcagacc tgaggctgac agggcagatt aaacagttct
      481 ggctggaatc gggatgcgtc tatggttatc gcaaaatcca tctggatctg cgggacagcg
      541 ggcaacagtg cggagtgaac agagtctggc gactgatgaa acgtgtcggg ataaaggctc
      601 aggtcggata ccggagcccg cgggcacgta aaggcgaggc cagtatcgtg tcgcccaaca
      661 ggctccagcg acagttcaat ccggatgctc cggataagcg ttgggtaacg gacataacct
      721 acatcaggac ccacgaaggc tggctgtatc ttgccgtggt tgttgatctg ttctcacgca
      781 aaattatcgg ctggtccatg caatcccgga tgacaaagga cattgtcctg aacgcactgc
      841 tgatggctgt atggcgccgt aatccccaaa aacaggtgct ggttcattcg gatcagggca
      901 gtcagtacac aagccatgag tggcagtcgt tcctgaaatc acacggcctg gagggcagca
      961 tgagccgtcg cggtaactgc catgataatg cggttgcaga aagctttttc cagttgttga
     1021 aacgcgaacg gataaagaaa aagatctacg gaacgcggga agaagcccgc agcgatattt
     1081 ttgattacat cgaaatgttt tataacagta agcgtcggca tggttccagc gatcagatgt
     1141 caccgacaga atatgaaaac cagtattatc aacggctcgg aagtgtc
//
LOCUS       DBBGLQ010000381         1184 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.388, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000381 DBBGLQ010000000
VERSION     DBBGLQ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.388"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACIYSB_005748"
     CDS             complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACIYSB_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HGC5432515.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(371..>1184)
                     /locus_tag="ACIYSB_005749"
     CDS             complement(371..>1184)
                     /locus_tag="ACIYSB_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGC5432516.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWYAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDIWGNPFYHQYLCVPDGKG
                     DYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRP
                     RYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNIERFIRFVFTGVM"
BASE COUNT          341 a          281 c          212 g          350 t
ORIGIN      
        1 tcaatgccca ggttacatat aataataaga gcaattaact taatttattt gtttataatg
       61 cgcttactta atacaaacca ttgaatatac cctcctgaca gaaaataaag ccatattatt
      121 atcgccataa tatacttact atcaatcctt acgccaataa tgtctgatac agtccctata
      181 aatattagag caataaagaa tactatgcct gttggaaaac atattaccat cattccataa
      241 gacatgagac taattaactc gctcacatca cgagtggagt ctaatagtga aagtctatgc
      301 atggaaaaga atagtattaa aagcgataaa gccagccata tagctctgaa taaaaaaata
      361 atatatttca ttacatcact ccggtgaata caaatctaat aaaacgttca atgttatttc
      421 cggagcactt catcttacat gtttctaaag aatcattaga ccagtcttgg cagtttttaa
      481 atagccctaa atttataggt gtgaatatat caggcaatac agaataacgc ggccttacct
      541 ctttaaactt cccttttaaa cagttctcca cacaactatt atcggtttca acgaggtcac
      601 aacggccagc agcttctttt gtatcattac ttgctttacc tggcccccat agaccatctc
      661 cttttgattc tccccgttgg tcttggccac cacaagtata gtccccttta ccatctggga
      721 cacaaagata ttgatgatag aacggattcc cccatatatc tggaccgctt ctttcaccag
      781 ttccgcccat tgaatgcagt ggcttaatgc attttagtgt ttctaatcct aatggatcta
      841 tatttgagat cggattcaac ggatactgat aaaaattcca tccccctttc agcccaatcg
      901 gatcctgagt gatatatcgc ccctgcagcg ggtcataata gcggtggcgg ttgtaataca
      961 ggccggactc ctcatcatac tgctgccccg gcaggcggat aagctgctgc agctgatgcg
     1021 ggttctcttc attcagcagg ttaccccatt catcgtattc tgcgtaccac gctgttgtcc
     1081 cttccgtgct gataagggcc agcggcaggc cgcgatggtc gcagtggtac aggtgaattt
     1141 ttcgcgccgg cgtgtatacc gggtccatct ggctttgcat ctgc
//
LOCUS       DBBGLQ010000382         1167 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.389, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000382 DBBGLQ010000000
VERSION     DBBGLQ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.389"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..80)
                     /locus_tag="ACIYSB_005750"
     CDS             complement(<1..80)
                     /locus_tag="ACIYSB_005750"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGC5432517.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLP"
     gene            complement(44..787)
                     /locus_tag="ACIYSB_005751"
     CDS             complement(44..787)
                     /locus_tag="ACIYSB_005751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGC5432518.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRCFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            complement(792..>1167)
                     /locus_tag="ACIYSB_005752"
     CDS             complement(792..>1167)
                     /locus_tag="ACIYSB_005752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGC5432519.1"
                     /translation="YARFLDAVNFVNGNSDADPEQEVISRWRIEQCSDLTAVSATFVL
                     ATPTETDGAVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLS
                     GCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          247 a          379 c          312 g          229 t
ORIGIN      
        1 gggagctgtg tggccagtgc ccggatggct tcagcccccg ttttcacacg aaggtcgatg
       61 cggcggccaa atcgttgcaa atccccgtaa aggcagatgc gtgccatgcc cggtgacgcc
      121 agagggagtg tgtgcgtcgc tgccatttgt cggtgtacct ctctcgtttg ctcagttgtt
      181 caggaatatg gtgcagcagc tcgccgtcgc cgcagtaaat tgcggcatga ttcggcaccg
      241 atgaaccaaa acagcacagc agcacgtcgc cgggctgcgc cgctgacaac ggcacctgat
      301 acagccccgt cgcctccaga ttatccagat agagattctg gccgttacgc caccagtcat
      361 ccccgcgatg aaaatccggc atctcaattc ccgccagatg gtaagcatcc cggaacagcg
      421 tgtaacagtc cgtcaccccg tgctcaaagc accgcccggt gagatgcggc acacagcgga
      481 acttgtgaat cgccccccgg cagaccagcc accacggcaa atcactctgc acctgcagcc
      541 gccggtcggc ctcactcagc cagggcagac caccggggtg actgtggacc agtgccacaa
      601 tctcaccctg catctctgcc tgcagccagt cctccggcga catccggaaa tacgcctccg
      661 gctcaccgga gatattcacg caggggaaat atctttcccc ctccggcgct ctcaccacga
      721 agccgcacga ctccgctggc gcacatcgcc gggcgtgcgc cagaatcgct gattctgtct
      781 ctgtcatgga attactgcga aagtttgtta atggaaagga agccgccaaa gttgccgacg
      841 ttattgcgga acttacagcc actcaggcat ttgctgcatt tatccttcgt gatatcggac
      901 gtcggctggt catattcatc cgcgactgcc ggaccgtgat aaccgcactc atcaccgcga
      961 taggtccagg tgcaggtgtt ggccagcata atacgtcccg gaaaaacagc gccgtccgtt
     1021 tccgtcggcg tggccagtac aaaggtcgcg ctgaccgctg tcaaatcact gcactgctcg
     1081 atgcgccagc ggctgatcac ctcctgctcc ggatcggcgt cactgtttcc gttgacgaag
     1141 ttcaccgcat ccagaaaacg ggcataa
//
LOCUS       DBBGLQ010000383         1153 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.390, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000383 DBBGLQ010000000
VERSION     DBBGLQ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.390"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            48..1028
                     /locus_tag="ACIYSB_005753"
     CDS             48..1028
                     /locus_tag="ACIYSB_005753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HGC5432520.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSH"
BASE COUNT          319 a          317 c          299 g          218 t
ORIGIN      
        1 gatatgagat catcatattc atccggagcg catcccagag ggacatcatg agccatcaac
       61 tcaccttcgc cgatagtgaa ttcagcacta agcgccgtca gacccgaaaa gagattttcc
      121 tctcccgcat ggagcagatt ctgccatggc agaatatgac cgctgtcatc gagccgtttt
      181 atcccaaggc gggcaatggc cgacggccct atccgctgga gaccatgctg cgtattcact
      241 gcatgcagca ttggtacaac ctgagcgacg gtgccatgga agatgccctg tacgaaatcg
      301 cctccatgcg cctgtttgcc cgattatccc tggatagcgc cctgccggat cgcaccacca
      361 tcatgaattt ccgccacctg ctcgagcagc atcaactggc ccgtcaattg ttcaagacca
      421 tcaatcgctg gctggccgaa gcaggcgtca tgatgaccca aggcactttg gtggatgcca
      481 ccatcattga ggcacccagc tctaccaaga acaaagagca gcaacgcgat ccggagatgc
      541 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
      601 ccaagagtgg cctgacccac agcctagtca ccaccgcggc caacgagcat gacctcaatc
      661 agctgggtaa tctgcttcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
      721 gagcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgtc
      781 ccggcaaggt aaaaaccttg aagcagcatc cgcgcaagaa caaaacggcc atcaacatcg
      841 aatacatgaa agccagcatc cgtgccaagg tagagcaccc gtttcgcatc atcaagcggc
      901 agttcggctt cgtgaaagcc agatacaagg ggctgctgaa aaacgataac caactggcga
      961 tgttattcac cctggccaac ctgtttcggg tggaccaaat gatacgtcag tgggagagat
     1021 ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt
     1081 caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag
     1141 ccgagaagaa ttt
//
LOCUS       DBBGLQ010000384         1142 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.391, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000384 DBBGLQ010000000
VERSION     DBBGLQ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.391"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..271
                     /locus_tag="ACIYSB_005754"
     CDS             <1..271
                     /locus_tag="ACIYSB_005754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGC5432521.1"
                     /translation="KVSTRTVTNRLRALGYSVIKCGDLHKNTVYSDRIVEIIRQLHDE
                     QGVTFREIAQRLSHELQPNPTPTLAQRLYWRRTASDAVFRELLPD"
     gene            595..813
                     /locus_tag="ACIYSB_005755"
     CDS             595..813
                     /locus_tag="ACIYSB_005755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HGC5432522.1"
                     /translation="MANRKQRRAIAAQRHIQTEINRRLFRAHNIARIMHANMLHERSN
                     ALSNLYSASVFSYLADDLRELQDLINQH"
     gene            886..>1142
                     /locus_tag="ACIYSB_005756"
     CDS             886..>1142
                     /locus_tag="ACIYSB_005756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432523.1"
                     /translation="MILADDFLEYLNNTERDLYARVLTRYEKYKLSLPEHQLREGDID
                     IEGRYIVRHLIAGYGIWRVTGREHSLLTICQSPSGVITEIVA"
BASE COUNT          286 a          349 c          282 g          225 t
ORIGIN      
        1 gaaagtttcg acacgcacgg taaccaaccg attgagggcg ctgggctaca gcgtgatcaa
       61 atgcggggat ctgcataaaa acaccgtcta cagcgaccgc atcgttgaga ttatcagaca
      121 gttacacgac gagcagggcg tgacgttcag ggaaatcgca cagcgcctta gccacgaact
      181 acagccgaac cctaccccga cactggctca acgtctctac tggcggcgca cagcatccga
      241 cgccgtattc cgtgaactgt taccagattg agcaatcacc atgtacacac aaaaaaaccg
      301 cttgcaacaa caaaccggaa cagggtacgc ttcccatgca cctcataaaa cgggtgtcgg
      361 gattctcaac ctgctgacaa acgaagcgca caaccgcgcc agcggttttt ttgtgcgtac
      421 tgtattgcca cgtctttttc gcatcagaat tatggtgggg cgtacggggc cgacttcggt
      481 cgggccggtt tcttcgtttg ccggtgttga gaaccccgta cgtctcgcca ccccgagatt
      541 ctcaactctg gttggtgagt atccaaaaac tcacaaacga agaggccaaa caccatggca
      601 aaccgcaaac agcgccgcgc tatcgctgcg cagcgtcaca tccagaccga aatcaaccgc
      661 agactcttcc gcgcccacaa catcgcgcga atcatgcacg ccaacatgct gcatgaacgc
      721 agtaacgcgt tatcaaacct ctattccgct tccgttttca gctatctggc ggacgacctg
      781 cgcgagctgc aagacctcat taaccagcac tgataaacaa cccgaagcca tcctgcgccc
      841 gttcgcggga ggcttccgca catctgtagt cgggaggatt gcgccatgat tctggctgac
      901 gattttctgg agtacctgaa caacaccgag cgcgacctgt acgcgcgcgt tctcacccgt
      961 tacgagaaat acaaactttc gctgccagaa catcaactcc gcgaaggcga tatcgacatt
     1021 gaagggcgct acatcgtcag gcacctcata gcgggctacg gcatctggcg tgtcaccgga
     1081 cgtgagcatt cattgctgac catctgccag agtccgtccg gcgtgataac ggagatcgtc
     1141 gc
//
LOCUS       DBBGLQ010000385         1118 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.392, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000385 DBBGLQ010000000
VERSION     DBBGLQ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.392"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..466
                     /locus_tag="ACIYSB_005757"
     CDS             <1..466
                     /locus_tag="ACIYSB_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432524.1"
                     /translation="NHAERRHEVMFRLWNSYWIEHISEVFNRRMDTVINAVILTSGAT
                     VFAGSNFSWLLGGIVAVLSGCNIAWKFGRRAQAAKEQAHRYNVLITESDGLSTEVLLA
                     RLVEIEKQDSPVLECMDNPARNKACAALGLNHRESLSLSEKIIASLTVGASR"
     gene            complement(507..719)
                     /locus_tag="ACIYSB_005758"
     CDS             complement(507..719)
                     /locus_tag="ACIYSB_005758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432525.1"
                     /translation="MTSKYTTNARKPGKEARHHIAVLRNLIMCEIDDFVAGLGQPGEP
                     ATPGEIHKELRRRIESAVDYVMNPND"
     gene            complement(716..931)
                     /locus_tag="ACIYSB_005759"
     CDS             complement(716..931)
                     /locus_tag="ACIYSB_005759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432526.1"
                     /translation="MSKPLTSIERKIVFMNVAGVQFEVDKQVIVSETRCPAAGEQCTY
                     LTLADGTQITAVTDTIRELVIIRGVKR"
BASE COUNT          217 a          276 c          302 g          323 t
ORIGIN      
        1 aaatcatgcc gaacggcgac acgaggtcat gtttcgtctc tggaacagtt actggattga
       61 acatatttcg gaggtgttta accgccgtat ggatacggtg attaatgctg tcatccttac
      121 gtctggggct actgtgtttg caggcagcaa tttcagttgg ttgctgggtg gtatcgtggc
      181 tgtgcttagt ggctgcaata tcgcctggaa atttgggcga cgcgctcagg ctgcaaaaga
      241 acaggctcat cgctataacg tcctgattac tgaaagcgac ggtctcagca cggaggtgtt
      301 gctggcgcga ctggtggaaa ttgagaagca ggactctccg gtgcttgaat gcatggacaa
      361 cccggcgcgc aataaagctt gtgctgctct tggtcttaat catcgcgaat ctctctcact
      421 ctccgaaaaa atcattgcca gccttacggt tggcgcttcc cgctaagcgt tatgcgtgcg
      481 tcatccggcg cgcgctcgcg ttctggtcag tcgttcgggt tcatgacgta atccaccgcg
      541 ctttctatgc ggcggcgtag ttctttgtgt atttcgcccg gtgttgctgg ctcgcccggc
      601 tggcccagtc ccgccacaaa gtcgtcgatt tcgcacatga tgagattgcg cagaacggca
      661 atgtgatgac gtgcctcctt gcctggcttg cgtgcgttgg tggtgtattt gctggtcatc
      721 gtttaacccc tctgataatc acaagctcgc gaatggtgtc ggtgacggcg gttatttgcg
      781 tgccgtctgc cagtgtcagg taggtgcatt gctcacctgc tgccgggcat ctcgtttcgg
      841 agacgatgac ctgcttatcc acttcgaact gcacgcctgc cacattcata aaaacaattt
      901 ttcgctcaat gctggttagt ggtttactca ttcgctgctc cttatttcag gcgtaaggga
      961 atccctccgc cgttgcggat gttttcttgc tttctggtaa tttccggtta tttatttatg
     1021 gcgcttgtcg ccagtaaata atgattcgat ggttttaatg ccgttaataa ttcgcatgat
     1081 tcccaccgcg caaataatgg cgaggatgag aattaacc
//
LOCUS       DBBGLQ010000386         1117 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.393, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000386 DBBGLQ010000000
VERSION     DBBGLQ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.393"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT          281 a          268 c          278 g          290 t
ORIGIN      
        1 cgtgaaaatt gtattcatcg tagtaaccac acttcacaat cctgttcgaa tcatatgtcg
       61 caacagaacc tgcgaagtaa tttggcttcc agccatgcgg gttttcagca ttgctgacgc
      121 tccaggtaca ctggctggca gaaaaaccag acggcaaagg tagtcgttct ccatttgcga
      181 tttttcctgt cagaacagtg ctacctgata ccctaatcca cgaaccggat tggcacggag
      241 gccagtgcca atccggtgtg tggagaactt ccggttcctc taacggtagt tacagtaatc
      301 ttggatcaca cagaggatca tttactggtc gcaatacggg aagtggtacg ctgtttgttt
      361 acgcctctgg agggaatggt ggttccgccg ggggagattg tgctaacaca tccagattgc
      421 agggatacgt tgcaggggca ttgattagca caaatgcgag taataaccca tcttatggga
      481 agacagcatt catctctttt gctgtccctg ctggcgcaac ttatcagatc acctcttatc
      541 cggcccaaaa ctactcttgt ggctcagggg tgttctcggt ttatgcatat cagatgtagc
      601 accgcattca actaaaccgt aatgaagttg aaacaatgtg ccagaaatca tctggcgcat
      661 aacgctacag agcgaaaaca gcttgtgatg gtccctgttc caccaacaat ccagcgattg
      721 ttcgcccagt cgggataagt tgagtccggt gagttgaacg ggttacggtt gtcctgcgac
      781 caggcggtta attgatatcc gcctgaaacc aaagtactac cactcggaca cactgcatag
      841 gacactgtat gtccacatcc actcgttccg taaacagtga aatgttcctt ttctttgtta
      901 cccttccacg aaccggattg gcacggaggc cagtgccaat ccggtttgtg gcttggaggc
      961 aataaagtga actattctgc ctgtaaatgg tatgaagcga cctccgcaca ttttatcggt
     1021 ggcagaagtg gaggttctat ttattacaag ccgattcagt gccctgccgg gtacgtcatg
     1081 acaggtacaa gaatgtacgg catcggagat ggtgttg
//
LOCUS       DBBGLQ010000387         1113 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.394, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000387 DBBGLQ010000000
VERSION     DBBGLQ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.394"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(284..554)
                     /locus_tag="ACIYSB_005760"
                     /pseudo
     CDS             complement(284..554)
                     /locus_tag="ACIYSB_005760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            856..>1113
                     /locus_tag="ACIYSB_005761"
     CDS             856..>1113
                     /locus_tag="ACIYSB_005761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HGC5432527.1"
                     /translation="MDDTQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAELT
                     HHLGHEKNQTKSGTNFRNGYSTKTVTTTDGPMELRTPPDRDG"
BASE COUNT          321 a          255 c          252 g          285 t
ORIGIN      
        1 atagcttatg atagttattg aatgtgacta tcagtgtaat taattatgca aattagaagg
       61 aatgttatgt gtgaagcgcc cctgtatccc gctcatactg gctaacccag acacgcagag
      121 tctgtggcgt acagccagca tttgaggcaa tggaacaaat taccccaatt gtgaatcata
      181 ctcatcctgt ttacctcttt ctcagggagt ttagtctcca ggattcccgg agcagttcaa
      241 agtgccctgt gctgcttaaa gtgccgctgc atgccaccac atggctgagc agcaacaggt
      301 attgttgttc cttcatgttg ttcacgcaac cagcgtaata ccaccggcga ggatggactg
      361 gcagtgtgat tacgttcagt aatacgttca taaaaggaag ttcagaatgc atgaatctgt
      421 tgtccgttga tgtttccttc tgtcgaattg tgtgtgaagt ggacaatcag aaaatgcggc
      481 cagtgatcca ctgcctgttg aataaaacgc cggatgtctc ccggctctgc aagtaatgct
      541 tcagtttcct gcactatttt atccctgttg aattttgtca aaccggtgca ggcacgcagg
      601 aaagtatgcc agcactggtg taatgccagt aacatacaat aagaatgaaa tgacgaatca
      661 gtcaggaaag ggtgccccca ttggcaaccc attgactgag cagaggatac aatcaggaag
      721 gttaacgaga gtatctccga catcaggggc aggattgatt tctacaatac cagcgccggc
      781 attgagcctg tacatagatt tatgtaattg cctgattttg atatgttcaa ttcaacatca
      841 aatgaaagct aaattatgga cgacacacaa ttgcaggctc tggctaacga actggccaaa
      901 aatctcaaaa cccctgaaga tctcagtcag ttcgatcgcc tgctcaagaa aataagtgtt
      961 gaggcagctc tcaacgctga actgactcac catctgggac atgaaaaaaa tcagaccaaa
     1021 tcgggaacca acttccgtaa tggctattcc acgaaaaccg tcaccactac cgacgggccg
     1081 atggaattac gtactccacc tgatcgtgat ggt
//
LOCUS       DBBGLQ010000388         1106 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.395, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000388 DBBGLQ010000000
VERSION     DBBGLQ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.395"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..116)
                     /locus_tag="ACIYSB_005762"
     CDS             complement(<1..116)
                     /locus_tag="ACIYSB_005762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGC5432528.1"
                     /translation="MSNTAVLDENGIATFAGDITVYHYDEETREYTSSSVEYL"
     gene            complement(116..>1106)
                     /locus_tag="ACIYSB_005763"
     CDS             complement(116..>1106)
                     /locus_tag="ACIYSB_005763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGC5432529.1"
                     /translation="VKAAYDLAARKAPASHTHPWNQITGVPSASLTAKGTVQLSSATN
                     SNSETLAATPRAVKAAYDLAASKASASHTHPWNQITGVPSASLTAKGIVQLSSATNSN
                     SETLAATPRAVKAAYDLANSKASAAHTHRWAQITDAPVFSSVARVIMGKQSIKDILDY
                     LGLGEGSTLPVGVPVPWPSSRPPEGWLQCNGAAFTRTKYPKLAVAYPDLRLPDLRGEF
                     IRGWDDLRMIDRGRLLLSTQEATYICTAIQAYHGVAGGADIQAGISFASHDNDIINIT
                     PDQPRTGNGILYGRKPVDRWAIARAGKLVSNEKDRWIAIRPRNIAFNYIVRAV"
BASE COUNT          226 a          272 c          299 g          309 t
ORIGIN      
        1 agatactcca cagaggatga ggtgtattcc cgggtttcct catcatagtg atatacagtg
       61 atatcgcctg cgaaagtggc gataccgttt tcatccagaa ctgcagtgtt actcattaaa
      121 ctgccctcac aatataatta aaagcgatat tgcgcggtct gatcgctatc catctgtctt
      181 tttcattact caccagctta ccggctctgg ctattgccca tctgtcaact ggttttctgc
      241 cgtacaggat gccgttaccg gttctgggtt gatcaggggt tatgtttatg atgtcgttat
      301 catgtgatgc aaatgaaatc cctgcctgaa tatctgcacc tcccgctacg ccgtggtacg
      361 cctgtatggc ggtacaaata tatgttgctt cctgtgtgga taacaataag cgtcccctgt
      421 caatcattcg caggtcatcc catccacgaa taaattcccc gcgcaaatct ggtagcctta
      481 ggtcagggta ggccactgcc agttttgggt attttgtacg cgtgaatgcg gcaccattgc
      541 actgtaacca cccttcaggt ggtcttgacg aaggccacgg aacgggtacg ccaactggca
      601 gcgtcgagcc ttctcctaaa ccaaggtaat caagaatatc ttttatgctt tgtttaccca
      661 tgatgacgcg ggcaacagac gaaaaaacag gggcgtctgt gatctgagcc cagcgatgag
      721 tgtgggctgc cgaggctttg ctgttggcaa ggtcatacgc agctttaaca gctcttggtg
      781 tggccgccag cgtttcggaa ttgctgtttg tggcgctgct tagctgtacg atgccttttg
      841 ccgtcagcga ggctgatgga acacctgtta tctgattcca cggatgggtg tggctggcag
      901 aggctttact ggctgcaagg tcatatgcag ccttgacggc tcttggtgtg gccgccagcg
      961 tttcggaatt gctgtttgtg gcgctgctca gctgtacggt gccttttgcc gtcagcgagg
     1021 ctgatggcac accagttatc tgattccacg ggtgggtgtg gctggcgggt gctttacggg
     1081 ctgcaaggtc atatgcagcc ttgacg
//
LOCUS       DBBGLQ010000389         1091 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.396, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000389 DBBGLQ010000000
VERSION     DBBGLQ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.396"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..528
                     /locus_tag="ACIYSB_005764"
     CDS             <1..528
                     /locus_tag="ACIYSB_005764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HGC5432530.1"
                     /translation="SRLGNDYRPTSAYERGQRYASRLQNEFAGNISALADAENISRKI
                     ITRCINTAKLPKSVVALFSHPGELSARSGDALQRAFTDKEELLKQQASNLHEQKKAGV
                     IFEAEEVITLLTSVLKTSSASRTSLSSRHQFAPGATVLYKGDKMVLNLDRSRVPTECI
                     EKIEAILKELEKTAL"
     gene            818..989
                     /locus_tag="ACIYSB_005765"
                     /pseudo
     CDS             818..989
                     /locus_tag="ACIYSB_005765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          287 a          238 c          259 g          307 t
ORIGIN      
        1 tccagattgg gtaacgatta tcgcccaaca agtgcttatg aacgtggtca gcgttatgca
       61 agccgattac agaatgaatt tgctggaaat atttctgcgc tggctgatgc ggaaaatatt
      121 tcacgtaaga ttattacccg ctgtataaac acagctaaac tgccaaaatc agttgttgct
      181 cttttttctc atcctggtga actatctgcc cggtcaggtg atgcacttca aagagctttt
      241 acagataaag aagaattact taagcaacag gcatctaacc ttcacgagca gaaaaaagca
      301 ggggtgatat ttgaagctga agaagtcatc actcttttaa catctgtgct taaaacgtca
      361 tctgcctcaa gaactagctt aagctcacga catcagtttg ctcctggagc gacagtgttg
      421 tacaagggcg ataaaatggt gctaaaccta gatagatctc gtgttccaac tgagtgtata
      481 gagaaaattg aagccattct taaggaactt gaaaaaacgg cactttgatg cggcatgttt
      541 tagtctacat ttatctgtct ttacttaatt tcctttagca caggctagaa aacataacta
      601 actggtctga atattatatc tgggaccacg gtcccatttg tattgtcggt ctgataatca
      661 gtttgggacc acggtcccac ttgtattgtc ggtctgatta tcggcctggg accacggtcc
      721 cacttgtatt gtcggtctga taatcagttt gggaccacgg tcccactcgt attgtcgatc
      781 agattatctg cgtgagacta cgattccatt aatgcctgta agtgagtgtg tagagcatat
      841 cctggcaagt ggcctggtct gtatgtgctg tgaatatcgg gttaaccgcc ccgccgcttg
      901 tttgacctca atattctgcg ggagttgtcc tggaggcacg ggatgaaatc cgcagcatga
      961 tggatgcctt ggcaaaaatc cggaaatgat gacgatggcg gtccccggac agagcacaaa
     1021 ggaaatcgcc gccagctccg ctttacccgg ccatgcaggg catggccttg tgggttttca
     1081 gttatgtggc c
//
LOCUS       DBBGLQ010000390         1088 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.397, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000390 DBBGLQ010000000
VERSION     DBBGLQ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.397"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..438
                     /locus_tag="ACIYSB_005766"
     CDS             <1..438
                     /locus_tag="ACIYSB_005766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGC5432531.1"
                     /translation="HKVGYAICRSADLNSWEYVPDHRGEVVYNTETGESKEITAPGDY
                     PENTTTIVPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKL
                     QAGRKLTQAETTRLNAVLDYIDAVTVTDTSTAPDVIWPELPEA"
     gene            complement(410..853)
                     /locus_tag="ACIYSB_005767"
     CDS             complement(410..853)
                     /locus_tag="ACIYSB_005767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGC5432532.1"
                     /translation="MNKVKSVYSPSENAIYNAALYESYIKEGTWPQDGIEISDEDVVR
                     FNGGNKPTGKMLGMVSGALAWVDEPPLSPEQKISDAENMKATFRAKADSEISWRQDAV
                     DAGIATDEETSTLTQWKKYRVLLMRVDTSTAPDIEWPTPPAVQAR"
     gene            complement(858..>1088)
                     /locus_tag="ACIYSB_005768"
     CDS             complement(858..>1088)
                     /locus_tag="ACIYSB_005768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432533.1"
                     /translation="SLVSRIPGAVHVVFPLPFPARVESITLTMADIQESLLSPATMPA
                     YGVNSTGTSRTGFTARMSGAGGFNLCYIAKGR"
BASE COUNT          288 a          271 c          257 g          272 t
ORIGIN      
        1 cataaggttg gttatgcaat ctgccgttcg gcagatttaa actcatggga atatgtgcca
       61 gaccatcgcg gtgaagttgt ctataacacc gaaacgggag aatcaaaaga aatcacagct
      121 ccgggtgatt accctgaaaa tacaaccact atcgtcccgt taacgccata cgataaatgg
      181 gatggtgaga aatgggtgac agatactgag gcacagcata gtgccgcagt agacgcggca
      241 gaagcacagc gccagtcgct gattgatgct gcaatggctt ccatcagtct gattcaactg
      301 aaattacagg ccgggcggaa gctgacgcag gcagaaacaa cccgacttaa cgccgtgctg
      361 gattacattg acgcggtgac ggtaacagat accagcaccg cgccggatgt catctggcct
      421 gaactgccgg aggcgtaggc cattcaatat cgggtgctgt tgaagtatca acacgcatca
      481 gcagcacacg gtatttcttc cattgggtga gagttgaagt ttcttcatca gttgcaatgc
      541 ccgcatcaac agcatcctga cgccaggata tttcactgtc agcttttgca cgaaatgtgg
      601 ctttcatgtt ttctgcatct gatattttct gttctggtga aagcggaggc tcatcaaccc
      661 atgcaagggc ccctgaaacc attcccagca ttttgcctgt cggcttattt cctccattaa
      721 atctgacaac atcctcatcg ctgatttcaa taccatcctg cggccatgtc ccctccttga
      781 tataactttc atatagcgct gcgttgtaga ttgcattttc ggacggactg taaacacttt
      841 taactttatt cattctgtta tctccctttc gctatatagc aaagattaaa cccaccggcg
      901 cctgacatac gggctgtaaa acccgttctt gaagtaccag ttgagttaac tccataagca
      961 ggcatggttg ctggagaaag cagagactcc tggatatctg ccattgtcag tgtaattgat
     1021 tcgacccgcg cggggaaagg tagcgggaaa acaacatgaa ccgccccggg aatcctggag
     1081 actaaact
//
LOCUS       DBBGLQ010000391         1087 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.398, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000391 DBBGLQ010000000
VERSION     DBBGLQ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.398"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            18..>1087
                     /locus_tag="ACIYSB_005769"
     CDS             18..>1087
                     /locus_tag="ACIYSB_005769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HGC5432534.1"
                     /translation="MEPMYRRLTSPHSDLIIKTHPYAEIVWWGSTLKHFSPDDCISLE
                     RPVANGRLDTDTPLTLMAENGAGLFGSPGLEGHRNGLDASPVFRTISVVQAGESLTLT
                     SEDAVAGLRMVSELVLTSSGVLKMRHALTNLREGDWQVNRFAITLPVAERAEEVMAFH
                     GRWTREFQPHRVRLTHDGFVLENRRGRTSHEHFPALIAGAPGFSEQQGEVWAVHLGWS
                     GNHRMKCEVKTDGRRYLQAEALWMPGEKALKKDETVYTPWLYASYSPEGLNGMSQQYH
                     RFLRDEIIRFPEQKPRPVHLNTWEGIYFDHDPNYIMQMAGRAAALGVERFIIDDGWFK
                     GRNDDHAALGDWYLDEQKYPDGL"
BASE COUNT          236 a          295 c          334 g          222 t
ORIGIN      
        1 gaaaacggag atgtcttatg gaaccgatgt accgcaggct gaccagtccc cactcagacc
       61 tgattattaa aacccacccg tatgcagaga ttgtctggtg gggatccacc ctgaaacact
      121 tttcaccgga tgactgtatc agcctggaaa ggccggttgc gaatggtcgt ctggataccg
      181 acaccccact gacgctgatg gccgaaaacg gcgcgggact ttttggctcg ccggggctgg
      241 agggacaccg caacgggctg gatgcgtccc ctgtgttccg taccatcagc gtggtacagg
      301 ccggagaatc actcaccctg accagtgagg atgcagtggc gggtctgcgc atggtcagtg
      361 aactggtgct gacatcgtcc ggtgtgctga agatgcgtca tgccctgacg aacctccgtg
      421 aaggggactg gcaggtgaac cgcttcgcca tcacgctccc tgtggctgaa agggcagagg
      481 aagtcatggc cttccacgga cgctggactc gtgagtttca gccgcacagg gtccgtctca
      541 cacatgacgg ttttgtgctg gagaaccgcc ggggccgcac gtcccatgag cattttccgg
      601 cactgattgc cggtgccccc ggtttctcag aacagcaggg cgaggtgtgg gccgtacatc
      661 tgggctggag tggcaaccat cgcatgaaat gtgaggtcaa aactgacgga cggcgttacc
      721 tccaggcgga agcactgtgg atgcccggag aaaaagccct gaagaaggat gagaccgtgt
      781 acacgccgtg gctgtacgcc agctattccc cggaggggct caatggtatg agtcagcagt
      841 accaccgttt tctgcgcgat gaaatcatcc gttttcctga gcagaaaccc cgtccggtgc
      901 acctcaatac ctgggaaggt atttattttg accacgaccc gaactacatc atgcagatgg
      961 ccggacgtgc ggcagcgctt ggcgttgaac gtttcattat cgacgacggc tggtttaagg
     1021 ggcgcaatga tgaccatgcg gctctgggcg actggtatct ggatgaacag aagtatccgg
     1081 acggact
//
LOCUS       DBBGLQ010000392         1082 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.399, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000392 DBBGLQ010000000
VERSION     DBBGLQ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.399"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..286
                     /locus_tag="ACIYSB_005770"
     CDS             <1..286
                     /locus_tag="ACIYSB_005770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HGC5432535.1"
                     /translation="VQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQIMTTDAE
                     AAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLHAG"
     gene            336..704
                     /locus_tag="ACIYSB_005771"
     CDS             336..704
                     /locus_tag="ACIYSB_005771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGC5432536.1"
                     /translation="MSDSLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            794..>1082
                     /locus_tag="ACIYSB_005772"
     CDS             794..>1082
                     /locus_tag="ACIYSB_005772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGC5432537.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCTHSQLINSIDILR"
BASE COUNT          262 a          323 c          263 g          234 t
ORIGIN      
        1 ggtacagaag cgtaacttct ggcagttaca ggctgaaatc agtcaccggg gacgttactg
       61 ccatccgtac agcatggata tcaccgtcac ccggaacagt ccgactggtc agataatgac
      121 aaccgatgcg gaagccgcag tcagcgaagc cctgcgcgac ctggccttct ggttgtaccg
      181 tcaactggaa aacgagtacg actggctgac ctcagacgcc gctgtggatg aggccctgct
      241 catcaacgaa tacaccttta ccgaagccgg tctgcacgcc ggctgacctg tcatcgttaa
      301 accattctta accgattttc tgtaaggatt ttatcgtgtc agactcactc catgaaacaa
      361 attatcccga cgataacaac gaccgcccct ggtgggggct accctgcacc gtgacgccct
      421 gttttggggc acgtctggtg caggagggta accggttgca ttaccttgcc gaccgcgccg
      481 gtatcagagg ccggttcagc gacgcggatg cgtaccatct ggaccaggcc tttccgctgc
      541 tgatgaaaca actggagctc atgctcacca gcggtgaact gaatccccgc catcagcata
      601 ccgtgacact gtatgcaaaa gggctgacct gcgaagccga caccctcggc agttgtggct
      661 acgtttatat ggctgtttat ccgacgcccg aaacgaaaaa gtaactctcc agaataacct
      721 tctgctccgg cccggtgcgc tacagcacca ctctcctcaa aatttaatct ctcatatcag
      781 gaaaatcttc agtatgaaaa cattacccga tacacacgta cgggaggcat cgcgctgccc
      841 gtctcccatc accatctggc agacactgct cacccgactg ctggaccagc attacggcct
      901 cacgctgaat gacacaccgt tcgccgatga acgtgtgatt gagcagcata ttgaagctgg
      961 catttcactg tgtgatgcgg tgaactttct cgttgaaaaa tacgcgctgg tacgtaccga
     1021 ccagccggga ttcagcacct gtacccactc tcagttaata aacagcatcg atatcctccg
     1081 gg
//
LOCUS       DBBGLQ010000393         1075 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.400, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000393 DBBGLQ010000000
VERSION     DBBGLQ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.400"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..370)
                     /locus_tag="ACIYSB_005773"
     CDS             complement(<1..370)
                     /locus_tag="ACIYSB_005773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HGC5432538.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIET"
     gene            complement(816..1075)
                     /locus_tag="ACIYSB_005774"
                     /pseudo
     CDS             complement(816..1075)
                     /locus_tag="ACIYSB_005774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015242.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          307 a          226 c          191 g          351 t
ORIGIN      
        1 ctgtttcaat gcaatcagcg gcaagttgga gtggatttag atttttcaga tatggtctta
       61 tgatctttcc cttacttaat aaatagcatg tataggattc ggaaagtcca gcatctattt
      121 tgtacctcat tatattagat aaggatgtga gaaattcctt gttatcattc gcagccatat
      181 atgccagtcc gagttcagcc tctgcggcct tacaggcaaa atagcttagt gaacacgctt
      241 ttcgtccctc tttagtactg gctgatatta gtgcccataa tattgctttg tctgccaaat
      301 ctaactctct ctgaaattgt cttgcagtaa ctgcactcgc agaaatagag aaaaaaatac
      361 aattgttaat aaccttgcca tattttttag tccgtaactg ttaatgtgct atctggctct
      421 gaaaatatta actcatttaa tttttgcatg tcattagata aaccagttat acaaccctcg
      481 gaacagaatc gaggtcctaa agacggacca aaagcgttta aacaactatg tattgaaaat
      541 ccattgcgat tataagttcc tgtattaact gacggtacta atcgtatgcc gtcaggtttt
      601 ctattgcttg ctcccgggtc attaacattc caactccata ggccttgagg tattggccat
      661 ctatttataa atgtgtacaa cggggattat ttttacattg catattatta ccagaagcga
      721 ctggaatact aagcacatca taaaaattat catcacatgg tatatctgaa acacacgaaa
      781 gcatacctaa agttatgctg tataaacagg tggctaatcc caaggggatc gattccagaa
      841 atcgggttca acggatactg ataaaaattc cacctacctt ccagcccaat cggagcctga
      901 gtgttatacc gcccctgctg cggttaatgt taccgataat agttatagta cagccctgat
      961 tcatcatcat actactgctg cggcagtctg atgaactatt gcttttacgg attctcctta
     1021 ttcttccttc tatccattcg ctttagcttt ttgagctctt gaagtttgct ctttg
//
LOCUS       DBBGLQ010000394         1067 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.401, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000394 DBBGLQ010000000
VERSION     DBBGLQ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.401"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            89..541
                     /locus_tag="ACIYSB_005775"
     CDS             89..541
                     /locus_tag="ACIYSB_005775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HGC5432539.1"
                     /translation="MKKINILILCLFSGASWASDVDNVKCIRVDSKDIERLFDRWNES
                     LKTGDAKKVADNYLSDAILLPTVSKKIRITDAERVDYFEHFLKKKPVGEINFRTIRIG
                     CNMALDTGNYTFTFEDKKQVSARYSFAYEWDGKDWKISSHHSSVVPTT"
     gene            complement(750..1067)
                     /locus_tag="ACIYSB_005776"
                     /pseudo
     CDS             complement(750..1067)
                     /locus_tag="ACIYSB_005776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          322 a          213 c          213 g          319 t
ORIGIN      
        1 atagagaaca tcgtttttac tatcaaatag ttgcactgta ttaatatgat taaactttaa
       61 aaaagtttca ggatgatcag gaggagttat gaaaaaaata aatattttaa ttctgtgtct
      121 gttttctgga gcgtcgtggg caagtgacgt agataatgtt aagtgtattc gggtagatag
      181 taaagacatt gaaaggttat tcgatcgctg gaatgaatca ttaaaaacag gtgatgccaa
      241 gaaggttgct gataactatt taagtgatgc gatcttactg cccacagtat caaaaaaaat
      301 taggattaca gatgcagaaa gagttgacta ttttgagcat tttttgaaaa aaaaacctgt
      361 tggagaaata aacttcagaa ctattcgcat tggatgcaat atggctcttg atacagggaa
      421 ttacacgttt acttttgaag ataaaaaaca agtttcagca cgctatagtt tcgcttatga
      481 gtgggatgga aaggattgga aaatatcatc ccatcattca tctgtagtgc caacaacata
      541 atatattgag cctgtttacc attctctgtt aatgccttct cttaaaagtg accgtccaag
      601 cggtcactga ttacccattc ttaattcagt gcaggattaa aatatatgtg caaaataatt
      661 taattcagac agctttttat ttcactatgg ctatcccaga tatcgacgac ggatgaaaag
      721 tggctcttac gcaacctgga tggagatttt catgccagtg gactcatgac cctctgcctg
      781 agtagttcaa atccggctcg accatacatc tggcctttca gcatttttag tcgatttaca
      841 tgactttcga caacccccat tattccagcg actgctgatt gcctcacata tagccgcagc
      901 atcagctgcc atccttgcag ccacgcgccg aagttaccac tttctcgttg ctcttttaac
      961 cactccctcc atgggtccag aagtcctcgc tctggaggcc gggttgacat ttcagggaat
     1021 gttcctgaac acacccagag actgactgtc acacgtgata agcctgt
//
LOCUS       DBBGLQ010000395         1049 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.402, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000395 DBBGLQ010000000
VERSION     DBBGLQ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.402"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..763
                     /locus_tag="ACIYSB_005777"
     CDS             <1..763
                     /locus_tag="ACIYSB_005777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGC5432540.1"
                     /translation="HFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPHHLHQ
                     PYRLPGQQHDEESGLYYNRHRHYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPM
                     GLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHC
                     YLMCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSD
                     ACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
     gene            747..>1049
                     /gene="ydcD"
                     /locus_tag="ACIYSB_005778"
     CDS             747..>1049
                     /gene="ydcD"
                     /locus_tag="ACIYSB_005778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HGC5432541.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKTPKSV
                     EIYLSEKNIIYKIINDQKISRGNGHFISIMVNNYRTHCGVVDINLNFFNDILYSVRL"
BASE COUNT          316 a          185 c          255 g          293 t
ORIGIN      
        1 tcatttttac cactgcgacc accggggcct gccgctggcg ctcatcagcg aagacggcaa
       61 tacggcgtgg cgcggggagt atgatgaatg gggcaaccag cttaatgagg agaacccgca
      121 tcacctgcac cagccgtacc gtctgccagg gcagcagcat gatgaggagt cggggctgta
      181 ctataaccgt caccggcact acgatccgtt gcaggggcgg tatatcaccc cggacccgat
      241 tgggttgaga ggtggatgga atatgtatca gtatccgttg aatcccatac aagtgataga
      301 cccaatgggg ttagatgcga ttgagaatat gacatcaggt ggactaattt atgccgtatc
      361 tggtgtacct ggattgattg ctgcaaacag cattactaac agtgcttacc agttcggtta
      421 tgatatggat gctattgttg gcggagctca taatggggcc gccgatgcaa tgagacattg
      481 ttacttgatg tgtcgaatga ctaagacatt tggatcaaca atagctgacg tgataggtaa
      541 aaatcatgag gcggcagggg atagacaagg tcagccagct aaagaaagaa tcatggatct
      601 taaaaataac actgtcggta ttgcttgtgg cgatttttct gccaaatgta gcgatgcatg
      661 tattgaaaaa tataacactg ggcaactctt cgggttagat ggtataaaag cagataatcc
      721 aataaaagca aagcaaggga gttcagatgc ttcaaattat tagaggcaaa cttgtcattt
      781 ttttaattac cctttgttta tttgttgttt accttgggtt tgataacaat tcaaattctg
      841 acatcgtatt ttatggacat aaaacaccaa agagcgttga gatatatctt tctgaaaaaa
      901 atattattta taaaataatt aatgaccaaa aaattagtag agggaatggt cattttataa
      961 gtataatggt taataattac aggacgcact gtggagtggt tgatataaat cttaattttt
     1021 tcaatgatat tctttatagc gtgcgatta
//
LOCUS       DBBGLQ010000396         1049 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.403, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000396 DBBGLQ010000000
VERSION     DBBGLQ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.403"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..286
                     /locus_tag="ACIYSB_005779"
     CDS             <1..286
                     /locus_tag="ACIYSB_005779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGC5432542.1"
                     /translation="FSRRTPFRKPASSDARPSSDDTPLSCGCSETRKASDKKPGEAAV
                     LWIKRGDVEALQKARNRASYLVKYETKQHDGSGQRNYGCSRGPGRLLDGR"
     gene            643..>1049
                     /locus_tag="ACIYSB_005780"
     CDS             643..>1049
                     /locus_tag="ACIYSB_005780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432543.1"
                     /translation="MNAIPYFDYSMAPFWPSYQSKVIGVLERALREQPCSRIRRILLR
                     LPGEHGNIFSDRKTWFGMAFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQ"
BASE COUNT          226 a          280 c          278 g          265 t
ORIGIN      
        1 tttttccagg cggacgcctt tccgtaaacc ggcatcatct gatgctcgcc cttcttccga
       61 tgatacgcct ttgtcgtgtg gatgttctga aaccaggaag gcttctgaca aaaagccggg
      121 tgaagccgct gttctctgga tcaagcgtgg tgatgtggaa gcgctgcaga aagcccggaa
      181 cagagccagt tatctcgtga aatatgagac gaagcagcat gacggttctg gtcaacgtaa
      241 ttatggttgc agtcgtgggc cggggcgtct gctggatggc aggtgagccc tgtaaaacga
      301 catccggtgc ctgagcttat gctacaggac gttggctgac gcccttagct cgttttctga
      361 aaaagtcgtc ctgaagtcat gcaacggtat ttttctctgc tttcgctcct ggaaacactc
      421 cgtcggtaaa accttactgt cctgcttaat gatgtgaacc gaagtcaacg gagatcattc
      481 attctgaacc tgcatccggt gttctgtccc ttgtcggtta cctgatcttc ccgttctgcc
      541 gcggtttttc acttattcca tcaaccccat acgcaagcca taacacgtca gcgcatccac
      601 gggcaggacg cgttgtgggc tgcgtataac ggaacatatc ttatgaatgc tatcccttat
      661 ttcgactata gcatggcgcc cttctggcca tcttatcaga gcaaagttat cggtgtcctt
      721 gagcgtgcgc tgcgtgagca gccctgctcc cgaatacggc ggatcctgct tcgtctgccg
      781 ggggaacatg gcaacatctt cagcgacaga aaaacctggt tcggtatggc ctttatcgaa
      841 accgtcagtg cgctgatgaa tgcaaccccc ggacgtgatc tctgctggct cctgactcgt
      901 catcctgaaa aaccggaata ccacgtggtg ctgtgcgtca gacaggagta tttcgacggc
      961 cctgaactgg atcggctgat tctggatgcc tggagcaacg tactgggctt cgcgtcaccg
     1021 ggtgaagcgg caccgtacca gaaacagat
//
LOCUS       DBBGLQ010000397         1047 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.404, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000397 DBBGLQ010000000
VERSION     DBBGLQ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.404"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            14..235
                     /locus_tag="ACIYSB_005781"
     CDS             14..235
                     /locus_tag="ACIYSB_005781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGC5432544.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            315..683
                     /locus_tag="ACIYSB_005782"
     CDS             315..683
                     /locus_tag="ACIYSB_005782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGC5432545.1"
                     /translation="MSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKRLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            773..>1047
                     /locus_tag="ACIYSB_005783"
     CDS             773..>1047
                     /locus_tag="ACIYSB_005783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGC5432546.1"
                     /translation="MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINS"
BASE COUNT          244 a          298 c          255 g          250 t
ORIGIN      
        1 gaggactatt ctcatgaaaa tcatcacccg tggtgaagcc atgcgtattc accgtcagca
       61 tcctgcatcc cgtctttttc cgttctgtac cggtaaatac cgctggcacg gtagcacgga
      121 tacatatacc ggccgtgaag tacaggatat tcccggtgtg ctggctgtgt ttgcagaacg
      181 ccgtaaggac agttttggtc cgtatgtccg gctgatgagc gtcaccctga actgagtctg
      241 gaagggcatt cagaagagca gaattatcgc caccaccgga ccattcttaa ccaattttct
      301 gtgaggattt tatcgtgtca gacacactcc ccgggacaac gcttcccgac gacaataacg
      361 accgcacttg gtggggactg ccctgcaccg tgacgccctg ttttggggca cgtctggtgc
      421 aggagggtaa ccggttgcat taccttgcag accgcgccgg tatcagaggc cggttcagcg
      481 acgcggatgc gtaccatctg gaccaggcct ttccgctgct gatgaaacaa ctggaactca
      541 tgctcaccag cggtgaactg aatccccgcc atcagcatac cgtcacgctg tatgcaaaaa
      601 ggctgacctg cgaagccgac accctcggca gttgtggcta cgtttatatg gctgtttatc
      661 cgacgcccga aacgaaaaag taactctcca gaataacctt cttctccggc ctggtgcttt
      721 caccacgcca cttttccatt tttcatctct gcatatcagg aaaatcttca gtatgaacac
      781 attacccgat acacacgtac gggaggcatc gcattgccag tctcccgtca ccatctggca
      841 gacactgctc acccgactgc tggaccagca ttacggcctc acactgaatg acacaccgtt
      901 cgctgatgaa cgtgtgattg agcagcatat tgaggcaggc atttcactgt gtgatgcggt
      961 gaactttctc gttgaaaaat acgcactggt gcgtaccgac cagccgggat tcagcgcctg
     1021 tactcgttct cagttaataa acagtat
//
LOCUS       DBBGLQ010000398         1042 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.405, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000398 DBBGLQ010000000
VERSION     DBBGLQ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.405"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            242..>1042
                     /locus_tag="ACIYSB_005784"
     CDS             242..>1042
                     /locus_tag="ACIYSB_005784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter barrel
                     domain-containing protein"
                     /protein_id="HGC5432547.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWTYIDNPVTALD
                     WDHMDKAGTVDGNYVNYSGFVYYNNTNGDFDQSFNGDTVNGTISTYYLNHDYADSTAN
                     QLDISNSVIHGSITSMLPGGYYDRFDADGNNLGGYDFYTDAVVDTHWRDGDVFTLNIA
                     NTTIDDDYEALYFTDSYKDGDVTKHTNETFDTSEGVAVNLDVESNINISNNSRVAGIA
                     LSQGNTYNETYTTESHTWDNNISVKDSTVTSGSNYILDSNTYGKTGHFG"
BASE COUNT          315 a          193 c          201 g          333 t
ORIGIN      
        1 atttattatg cgtatgcttc aaaaacaaaa ttatctgcaa catgttgaaa tactgtgctt
       61 ttatgaattt gatgcgtgtt tttctccata aattatatat gtccacattc ggacttaggg
      121 gaaagaataa ttgaaccatt cgtctgtaac gcagcataat cgttagcgcg aaacataata
      181 tgttttctat gcattgataa ttgatggatc aacttattac gtccctgagg agggatgaca
      241 aatgcactcc tggaaaaaga aacttgtagt atcacaatta gcattggctt gcactctggc
      301 tatcacctct caggctaatg cagcaaacta tgatacctgg acttatatcg ataatcccgt
      361 tacagcactt gattgggatc atatggataa ggcaggcact gtagatggca actatgtaaa
      421 ctatagtggt tttgtctatt acaacaacac caatggtgat ttcgatcagt cctttaacgg
      481 cgataccgtt aacggcacga tctcaaccta ttatttgaac catgattatg cagacagtac
      541 tgctaatcag cttgatatca gtaattcagt gattcacggt tcgattactt ctatgctgcc
      601 tggcggttat tatgatcgtt ttgatgcaga tggtaataat ctgggtggat atgattttta
      661 cactgatgcg gttgttgata cacactggcg tgatggtgat gttttcactt tgaacattgc
      721 taacactact attgatgatg attatgaagc tctttacttc actgattctt ataaagatgg
      781 tgatgtaacc aagcacacaa atgagacatt tgatacaagt gaaggcgttg ctgttaatct
      841 tgatgtagaa agtaacatca atatttccaa taactcccgc gttgcaggta ttgcattatc
      901 tcaaggtaat acttacaacg aaacctacac taccgaatct catacttggg ataacaatat
      961 ctctgtaaaa gattccacag tgacttcggg ttcaaattat atcctggata gcaatactta
     1021 tggcaaaact ggtcactttg gc
//
LOCUS       DBBGLQ010000399         1036 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.406, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000399 DBBGLQ010000000
VERSION     DBBGLQ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.406"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..268
                     /locus_tag="ACIYSB_005785"
     CDS             <1..268
                     /locus_tag="ACIYSB_005785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head-binding protein"
                     /protein_id="HGC5432548.1"
                     /translation="ESTIVDLTKVHPSTHYNIIGSSVAITNIYGAPLGRDLIVRVSSG
                     DHSGVKYVAGSVETNGATDWAAVPSTNAIFKCIKGPAGKVMYQL"
     gene            complement(325..>1036)
                     /locus_tag="ACIYSB_005786"
     CDS             complement(325..>1036)
                     /locus_tag="ACIYSB_005786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432549.1"
                     /translation="LEHALVSIAAIYIYSGVSPRINLKFLAISLVSFSAMIAYVLYIL
                     TFTSESIGYTSSQYTDLPVIFELFLMRIIANGDAYYFSLPYDTINRIVVENPFIQLFG
                     YIGGNGFVKTVFSYNYFDNDVGRLIWKQWYPWDDIARGPTSHFDLAGYVFFGFIGSLI
                     MTSIIGFIIGRISKAKYKNSGCMLPFTSSFLAVFYIRSLQLLLSPPVGIAYVVDMSVF
                     LVAFSFVLLCLRFARTQR"
BASE COUNT          369 a          190 c          184 g          293 t
ORIGIN      
        1 agaaagcact attgtagatt taacaaaagt tcatccatca actcattata atataatcgg
       61 ctcttcggta gctattacta acatttatgg tgctccacta ggcagagacc tgattgtacg
      121 ggtgtctagt ggtgatcatt ctggtgttaa gtacgtagcc ggttctgtcg aaactaatgg
      181 agctactgat tgggccgcgg tgccaagcac aaacgcgata ttcaaatgca ttaaaggacc
      241 ggctggtaaa gtgatgtatc agttataaac tagtggtgtg tgttttaaat aaaccacctc
      301 taaatgaggt ggtttttgca aaagttatct ttgagttctc gcaaatctaa ggcatagcaa
      361 cacaaaagag aatgccacta aaaatacaga catatcgaca acataagcaa tgccaacagg
      421 aggcgataac agtagctgca aagatctaat ataaaaaaca gccaaaaacg aagatgtaaa
      481 tggaagcatg caacctgagt ttttatactt tgcttttgaa atccttccta ttataaaacc
      541 tataatagac gtcataatta agcttccaat aaatccaaaa aacacatatc ctgcaagatc
      601 aaagtgactt gttggacctc ttgcaatatc atcccatggg taccattgtt tccatatcaa
      661 tctacctaca tcattatcaa aatagttata tgagaacacg gtttttacaa atccatttcc
      721 acctatatat ccaaatagct gaataaaagg gttttcgaca actattctat tgatagtgtc
      781 gtaaggcaga gaaaaatagt aagcatctcc atttgcaatt attctcataa ggaatagctc
      841 aaatataaca ggcaagtctg tatactgaga actggtataa cctatagatt cagatgtaaa
      901 cgtcaaaata taaagaacat atgcaatcat ggctgaaaaa gatacgagac tgattgccaa
      961 gaactttaaa tttattcttg gagacacacc tgaatatata tatattgccg ctattgatac
     1021 tagcgcgtgt tcaaga
//
LOCUS       DBBGLQ010000400         1029 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.407, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000400 DBBGLQ010000000
VERSION     DBBGLQ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.407"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..583
                     /locus_tag="ACIYSB_005787"
     CDS             <1..583
                     /locus_tag="ACIYSB_005787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432550.1"
                     /translation="LAAMLGCVSVNTANATALPAQFRAGQQVMNNAGGDHSQAAIMDF
                     CKREGIPLRPVGTQFIGKTDFCVFAYTAYLTDKAITKTGYSTKDTLSRLSQGWQQFEV
                     YRQQGLGELLQPLFMLALVPEGQQFLVKKGMLRQSDIAGFDSMMAYERKLTEQRNKKP
                     SASCVQSKTAEYSAVAGPLAKQMAEQWCKKYGQ"
     gene            604..996
                     /locus_tag="ACIYSB_005788"
     CDS             604..996
                     /locus_tag="ACIYSB_005788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432551.1"
                     /translation="MKRLAIALLASFPLIASAGNLQVKCGRFDIKIYPDAIFLNGNKV
                     DNAHRKTESDVMSYVFQEYAEMGGTYTLYSLDVPSRGDMTLSHQWQNADGEALREVKT
                     EKCGTFHSFKGKAPNVKSVLEKQRAGEL"
BASE COUNT          303 a          203 c          261 g          262 t
ORIGIN      
        1 gctcgcggct atgttgggat gcgtatctgt taatactgcc aatgcaactg cgttacctgc
       61 acaattcagg gcagggcagc aggttatgaa taacgctggt ggagatcatt cacaagcggc
      121 aattatggac ttttgcaaga gggagggcat tccattacgg cctgttggaa ctcagtttat
      181 tggtaaaact gacttttgcg tttttgcata tacagcatat ctgacggaca aagctataac
      241 aaaaacaggt tattcaacca aagatactct ttccagatta agtcagggat ggcagcaatt
      301 cgaagtgtat cgccagcaag gattaggtga gttattgcaa ccacttttta tgcttgcgtt
      361 ggtgccagag gggcagcaat ttttggttaa aaagggaatg cttcgccaaa gtgatattgc
      421 cgggttcgat agcatgatgg cttatgagcg caaactaacc gagcagcgaa ataaaaaacc
      481 atctgcttca tgtgtgcaaa gtaaaacagc agaatacagt gcagtagccg ggcctttagc
      541 caaacaaatg gcagagcaat ggtgtaaaaa atacgggcaa taagttacgc agcggaggca
      601 gaattgaaaa gattggctat tgctttactg gcgtctttcc ctttgatcgc gagcgccgga
      661 aatttacaag ttaagtgcgg tcggtttgat ataaaaatct atccggatgc catatttctg
      721 aatgggaata aggttgataa cgcacaccga aaaactgaat ctgacgtaat gagctatgtg
      781 ttccaggagt atgcggagat ggggggaact tacacgctgt actcgctgga tgttccttct
      841 cgcggtgata tgacattatc ccatcaatgg cagaacgccg atggcgaagc tctgcgcgag
      901 gttaaaacag aaaaatgtgg cactttccat tcttttaaag ggaaagcgcc aaatgttaaa
      961 tctgtgcttg aaaaacagcg cgcaggtgag ctttaaacac ctttaaccac ggagatgccg
     1021 ttccctgca
//
LOCUS       DBBGLQ010000401         1013 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.408, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000401 DBBGLQ010000000
VERSION     DBBGLQ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.408"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..404
                     /locus_tag="ACIYSB_005789"
     CDS             <1..404
                     /locus_tag="ACIYSB_005789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432552.1"
                     /translation="TWFGMDFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQ
                     EYFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSRSPDCEERLKELIWA
                     FSDFARDRRGVLDPEACYLAGNPWYPVAGQF"
     gene            473..721
                     /locus_tag="ACIYSB_005790"
     CDS             473..721
                     /locus_tag="ACIYSB_005790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5432553.1"
                     /translation="MLTSITGHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEGSRGGCSIVQD"
BASE COUNT          289 a          218 c          242 g          264 t
ORIGIN      
        1 agacctggtt tggtatggat tttatcgaaa ccgtcagtgc gctgatgaat gcaacacccg
       61 gacgtgacct ctgctggctc ctgactcgtc atcctgaaaa accggaatac cacgtggtgc
      121 tgtgcgtcag acaggagtat ttcgacggcc ctgaactgga tcggctgatt ctggatgcct
      181 ggagcaacgt actgggcttc gcgtcaccgg gtgaagcggc accgtaccag aaacagataa
      241 cccgggatgt ggtgctggac agtcgttcac cggactgcga agagagactc aaagagttga
      301 tatgggcgtt cagtgatttc gcccgcgatc gtcgtggtgt actcgatccg gaagcctgtt
      361 acctcgccgg taacccctgg tatcccgtag caggccagtt ctgaaagctc agttcagcag
      421 attcacccgc atttgcccaa cctttataaa tcgcacagag gatatcctta ctatgttgac
      481 ctcaataaca ggccacgaca gtgtattgct gcgtgccgac gatcccctga tcgacatgaa
      541 ctacatcacc agcttcaccg gtatgactga caaatggttt tacaagttga tcagtgaagg
      601 gcattttcct aaacccatca agctggggcg cagcagccgc tggtacaaaa gtgaagtaga
      661 gcagtggatg cagcaacgaa tcgagggatc aagggggggc tgcagtattg ttcaggatta
      721 gtattatcag ttgacgctat ggaaataata gaaaaatcaa ataacttgag tgaatttaat
      781 gaagtcgtag aggttctaat tagtatacct ctggtaaaac tatattgttt ggtaaaagaa
      841 aggtctgctg aaacgtttaa tttccattac aacagtaaac ttatactcag atgatgtaat
      901 atcgacattt gagataaatt gtgaagataa aagagaagcc atatttatta tatatggctt
      961 ctcataataa ctcatctatc taataaattt ttaacgtaat taaccaattc ttt
//
LOCUS       DBBGLQ010000402         1008 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.409, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000402 DBBGLQ010000000
VERSION     DBBGLQ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.409"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            12..980
                     /locus_tag="ACIYSB_005791"
     CDS             12..980
                     /locus_tag="ACIYSB_005791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HGC5432554.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVP
                     LRCPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRI
                     WRKLHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETA
                     MYRMKQLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
BASE COUNT          253 a          265 c          279 g          211 t
ORIGIN      
        1 cttttgctga gttgaaggat cagatcacgc atcctcccga caacacagac cattccgtgg
       61 caaagcaaaa gttcagaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
      121 gctccctcac tttctggctg gatgatgagg cgattcaggc ctggtatgag tcggcaacgc
      181 cttcatcacg aggaaggccc cagcgctatt ctgatctcgc catcaccacc gttctggtga
      241 ttaaacgcgt attccggctg accctgcggg ctgcgcaggg ttttattgat tccatttttg
      301 ccctgatgaa cgttccgttg cgctgcccgg attacaccag tgtcagtaag cgggcaaagt
      361 cggttaatgt cagtttcaaa acgtccaccc ggggtgaaat cgcacacctg gtgattgatt
      421 ccaccgggct gaaggtcttt ggtgaaggcg aatggaaagt cagaaagcac ggcaaagagc
      481 gccgtcgtat ctggcgaaag ttgcatcttg ctgttgacag caacacacat gaagttgtct
      541 gtgcagacct gtcgctgaat aacgtcacgg actcagaagc cttcccgggc cttatccggc
      601 agactcacag aaaaatcagg gcagccgcgg cagacggggc ttacgatacc cggctctgtc
      661 acgatgaact gcgccgcaaa aaaatcagcg cgcttattcc tccccgaaaa ggagcaggtt
      721 actggcccgg tgagtacgca gaccgcaacc gtgccgttgc taatcagcgg ctgagcggaa
      781 gcaatgcacg gtggaaatgg acaacggaat ataaccgtcg ctcgatagcg gaaacggcaa
      841 tgtacagaat gaagcagttg ttgggagatt cactgacgct gcgtgactac gatggtcagg
      901 tagcggaagc tatggccatg gtgcgtgcgt tgaacaggat gacaaaggct gggatgccag
      961 aaagcgtgcg tattgcctga aaatccagcc agctacaggg tcgttcgc
//
LOCUS       DBBGLQ010000403          993 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.410, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000403 DBBGLQ010000000
VERSION     DBBGLQ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.410"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..774
                     /locus_tag="ACIYSB_005792"
     CDS             <1..774
                     /locus_tag="ACIYSB_005792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5432555.1"
                     /translation="LKEQYGDNPLALNNVMSEQKKTWAAEDLLRGSWMAGLKSGWSEW
                     EESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILVKQAMV
                     GIVGSIGSAIGGATGGGSSASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEF
                     VFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGT
                     NGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            771..>993
                     /locus_tag="ACIYSB_005793"
     CDS             771..>993
                     /locus_tag="ACIYSB_005793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432556.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWT"
BASE COUNT          218 a          231 c          339 g          205 t
ORIGIN      
        1 ctgaaggaac agtatggcga taatccgctg gcgctgaata acgtcatgtc agagcagaaa
       61 aagacctggg cggctgaaga cctgcttcgc gggagctgga tggcaggcct gaagtccggc
      121 tggagtgagt gggaagagag cgccacggac agtatgtcgc aggttaaaag tgctgccacg
      181 cagacctttg atggtattgc gcagaatatg gcggcgatgc tgaccggcag tgaacagaac
      241 tggcgcagct tcacccgctc cgtgctgtcc atgatgacag aaattctggt taagcaggca
      301 atggtgggga ttgtcgggag tatcggcagc gccattggag gtgctaccgg tggcggctca
      361 tcagcgtcag gcggtacagc cattcaggcc gctgcggcaa aattccattt tgcgaccgga
      421 ggatttacgg gaaccggcgg caaatatgag ccagcgggga ttgttcaccg tggtgaattt
      481 gtcttcacga aggaggcaac cagccggatt ggtgtcggca acctgtaccg cctgatgcgg
      541 ggctatgcgg aaggtggtta tgtgggcggt gccggaagtc cggcgcagat gcggcgggct
      601 gaaggcatta attttaatca gaacaatcac gtggtgattc agaacgacgg tacgaatggt
      661 ctgccaggtc cacagatgat gaaggcagtg tatgacatgg cccgcaaggg tgcccgtgat
      721 gaaattcaga cacagatgcg tgatggtggc ctgttctccg gaggtggacg atgaaaacct
      781 tccgctggaa agtgaaaccc ggtatggatg tggcttcggc cccttctgta agaaaggtgc
      841 gctttggtga tggctattcc cagcgagcgc ctgccgggct gaatgccgac ctgaaaacgt
      901 acagcgtgac gctttctgtt ccccgttggg aggccacggc gctggagtcg tttctggctg
      961 agcacggggg ctggaaggcc tttctgtgga cgc
//
LOCUS       DBBGLQ010000404          984 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.411, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000404 DBBGLQ010000000
VERSION     DBBGLQ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.411"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..579
                     /locus_tag="ACIYSB_005794"
     CDS             <1..579
                     /locus_tag="ACIYSB_005794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HGC5432557.1"
                     /translation="MKLLAGMSEFFIGYGFWLCGTIFALTLLIRFSFSRYTGPGRRML
                     DKAPPWSLYQTLHGVNYLFSISSMLQINIPLSQALLRMEKGAEKNKWLKERIQAIRKH
                     VLSGQNLATAMRNSGYDFPSRICINKLLLNSEREDRVATMALYADQWLEEAKKRVKRM
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            629..>984
                     /locus_tag="ACIYSB_005795"
     CDS             629..>984
                     /locus_tag="ACIYSB_005795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HGC5432558.1"
                     /translation="MNFRSCLYSYFALKKEKEEDKGLTLLEVAGALIIIGIVVSLAIS
                     QMSSTMSSSDNQRELKNLQTIATKMKQYKFQGQYTSSDYIKTLTQSGSLPADMIAGGN
                     KAKNAWGGDVTIKATA"
BASE COUNT          293 a          212 c          226 g          253 t
ORIGIN      
        1 atgaaattgc ttgccggcat gtcggaattt ttcatcgggt acggattctg gctctgtggg
       61 accattttcg ccctgacgct tctgatccgt ttttcattca gccggtatac cggccccggc
      121 cggaggatgc tggacaaggc tcccccctgg tcactgtatc agaccttaca cggggtgaat
      181 tatctgttca gtatttcatc catgctgcag attaatattc ctctttcgca ggcgctgctc
      241 cgaatggaaa aaggtgcaga aaagaataaa tggttaaagg agagaattca ggcaatacga
      301 aaacatgtat tatccggtca gaatctggcc acagcaatgc gaaattccgg ttacgatttt
      361 ccgtccagaa tatgcatcaa taaactgctc ctgaactccg aaagggagga cagagtggca
      421 accatggcac tctatgccga tcagtggctg gaagaggcaa aaaaacgggt aaaacgaatg
      481 ggattaatta ttaccggtat atcaggaggt tttgtcttct tttttattct taacatgata
      541 tctgccatct acagtatcag cgacatgctg aaacaataat cccgcataca gaatatttct
      601 gaaacatccc ataaaccagg agtgatatat gaacttcaga tcatgtcttt attcttattt
      661 tgctttaaaa aaagagaaag aggaggataa aggtttaacc ttactggaag ttgccggtgc
      721 actgattatc atcggtattg tggtatctct cgcaataagt cagatgtcca gcacaatgtc
      781 atccagtgat aaccagcgtg aactgaaaaa cctgcagact attgcaacaa aaatgaagca
      841 gtacaagttc cagggacaat ataccagcag tgattatatc aaaacgctga cgcagagtgg
      901 ttccctgccg gccgatatga tcgccggtgg taacaaggcc aaaaatgcct ggggcggtga
      961 tgtcaccatt aaggcaacag caga
//
LOCUS       DBBGLQ010000405          983 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.412, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000405 DBBGLQ010000000
VERSION     DBBGLQ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.412"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            32..400
                     /locus_tag="ACIYSB_005796"
     CDS             32..400
                     /locus_tag="ACIYSB_005796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGC5432559.1"
                     /translation="MSDTLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            491..901
                     /locus_tag="ACIYSB_005797"
     CDS             491..901
                     /locus_tag="ACIYSB_005797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGC5432560.1"
                     /translation="MKLLPDTHVREASCCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITLGSIRGRNDETFPDAGSRQR"
     gene            864..>983
                     /locus_tag="ACIYSB_005798"
     CDS             864..>983
                     /locus_tag="ACIYSB_005798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGC5432561.1"
                     /translation="MKLSLTLEADSVNVQALNMGRIVVDIDGVELTELINKVAE"
BASE COUNT          237 a          283 c          244 g          219 t
ORIGIN      
        1 ttcttaacca cttttcagag aggattttat cgtgtcagac acactctccg ggataactca
       61 tcccgacgac aatcacgacc gcccctggtg ggggcttccc tgcacagtga ggccctgttt
      121 tggcgcccgt ctggtgcagg agggtaaccg cctgcattac cttgccgacc gcgccggcat
      181 cagaggccgg ttcagcgatg cggatgcata ccatctggac caggcctttc cgctgctgat
      241 gaaacaactg gaactcatgc tcaccagcgg tgaactgaat ccccgccatc agcataccgt
      301 cacgctgtat gcaaaagggc tgacctgcga agccgacacc ctcggcagtt gtggttacgt
      361 ttatctggct gtttatccga cgcccgaaac gaaaaagtaa accaccggaa taaccttctg
      421 ctccggccag gtgttttcac cacgccactc tccccataat ttaatctctc atatcaggaa
      481 aatcttcagt atgaaattat tacccgacac acatgtacgg gaggcatcgt gctgcccgtc
      541 tcccgtcacc atctggcaga cactgctcat ccgtctgctg gaccagcact atggcctcac
      601 actgaatgac acaccgttcg ctgatgaacg tgtgattgag cagcatattg aggccggcat
      661 ttcactgtgc gatgcggtga actttctcgt tgaaaaatac gcactggtac gtaccgacca
      721 gccgggattc agcgcaggag ccccgtcgca gttaatcaac agcatcgata ttctccgggc
      781 ccgccgggca acaggcctga tgacacgtaa taactacaga atggtaaata acattaccct
      841 gggtagcatc cgggggcgaa acgatgaaac tttccctgac gctggaagcc gacagcgtta
      901 acgtacaggc gctgaacatg gggcgaattg tcgttgacat tgatggtgtt gagctcactg
      961 aactgattaa caaggtcgct gaa
//
LOCUS       DBBGLQ010000406          961 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.413, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000406 DBBGLQ010000000
VERSION     DBBGLQ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.413"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT          364 a          219 c          152 g          226 t
ORIGIN      
        1 tgcgccaggg cactgttgag aatactttca aaacgggata accagtaagc attgtgtatt
       61 ggttgattca tgatgagaga tctccgttga tttggattga cttcacatca ttaatcactg
      121 cggtaaaact ttacggcatc ccgccattga gcaacgccaa tggctcctga ttaaaacaga
      181 atgaatcccc cacgaagaaa aaataaacct acacgcagag taaaacacac aacaacggca
      241 taaatgtttt ttaaaaacta caaagcctga agggaaacgg atcaacagaa cgcgcaaaca
      301 aaacgtcaga aacgcaatca gaacgctttc tggcgctgta aatcaacagc aacaccacac
      361 ctacagctta accacacaac tcaatcacag agccgaacag cgcgattaaa acctgtttac
      421 agacaccccc gctccaagaa caaaaaccac caacatcaga atcgattttc ttatactgaa
      481 aacgttactt ttaactacgc tccataaaaa cggttaacca tcgataacgt aaacctcatc
      541 tgaatcaaaa caccagaacc aatcatcacg cttttcctga aaagagtctt gcgctgagaa
      601 aaaacaccag ggccagttaa tcatctcatc aatctcatcg ttacaaaatc atccataaaa
      661 ccatttaaca aaatcatcgt aagcacacag taaatcacgc caggttatct cagccggatt
      721 tgcgttttta cataaagaca cttgtctcag gttaacgact aaaaacacaa aaactaagaa
      781 aaggttaaac gtaacttcag aaaaatagaa tatcatcctg attacttaaa caggaaagat
      841 tgttttgtag cagctttaaa ctgattttag ctaacgtaaa gatttactaa actggatgtg
      901 tatcagatca aacaatgtgt acagcaatgc ttaatgaaaa cacttgtaga attgtttatg
      961 a
//
LOCUS       DBBGLQ010000407          957 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.414, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000407 DBBGLQ010000000
VERSION     DBBGLQ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.414"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>957
                     /locus_tag="ACIYSB_005799"
     CDS             <1..>957
                     /locus_tag="ACIYSB_005799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGC5432562.1"
                     /translation="VAQRTVQLEQKSAENSGFEFHGYARSGLLMNDAASSSKSGPYLT
                     PAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFKAMLADGQRDYNDWTGGSSN
                     LNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIHWLDSDVVFLAGTGGGIYDV
                     KWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAGPFQLMVSGLRAKDNDDRKD
                     ANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALLYGHGLGAEVKGIGSDGALL
                     SEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGDSYEW"
BASE COUNT          262 a          213 c          249 g          233 t
ORIGIN      
        1 gtagcacaga gaacagttca gctcgaacag aaatccgcag aaaacagtgg ttttgagttt
       61 catggctatg cccgttccgg gttactgatg aatgatgccg cttccagtag caaaagtggg
      121 ccgtatctga ctcccgcagg tgaaactggt ggagctgttg gccgtctggg aaatgaagcc
      181 gatacctatg tcgagttaaa tgtagaacat aaacaaacac tggataacgg tgcgaccaca
      241 cgctttaaag caatgttggc tgacggacaa agagattaca acgactggac tggcggctcc
      301 agtaacctga atatccgaca ggcttttgcc gaactgggcg cattaccaag ttttaccgga
      361 gcattcaaag acagtactgt ctgggctggt aaacgctttg atcgcgacaa ttttgatatt
      421 cactggttag actccgatgt cgtattttta gcgggaacgg gcggcggtat ctatgacgta
      481 aaatggaacg atacatttcg cagtaacttt tctctctacg gacgtaattt cggcgatctt
      541 gatgatatcg acaataacgt tcagaattac atcctcacca tgaatcatta tgcaggcccc
      601 ttccagttga tggttagcgg attacgggca aaagataatg atgatcgaaa agatgccaat
      661 ggtgatctca ttcaaactga tgctgcaaat actggcgtac atgcgttagt tggtctgcac
      721 aatgacactt tctatggcct gcgtgaaggg acggcaaaaa cagcactgct atatggccat
      781 ggcctgggtg cggaagtcaa agggattggc tccgatggcg ccctgctgtc tgaggcgaat
      841 acctggcgct tcgcatctta cggcacaaca cctctgggaa gcggttggta tgtcgcacca
      901 gcaattctcg cacaaagcag taaagatcgt tacgtcaaag gcgatagcta cgaatgg
//
LOCUS       DBBGLQ010000408          943 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.415, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000408 DBBGLQ010000000
VERSION     DBBGLQ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.415"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..192
                     /locus_tag="ACIYSB_005800"
     CDS             <1..192
                     /locus_tag="ACIYSB_005800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432563.1"
                     /translation="GLRANPVCGERLVHLTEVTAIWVLTGEHLTGEITAEELCLCTLP
                     VEMVPVAVIAPTHQIFRAM"
     gene            complement(364..753)
                     /locus_tag="ACIYSB_005801"
                     /pseudo
     CDS             complement(364..753)
                     /locus_tag="ACIYSB_005801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          233 a          231 c          250 g          229 t
ORIGIN      
        1 ggcctccgtg ccaatccggt gtgtggcgaa cgattggtgc acctgacgga agttacagca
       61 atctgggttc tcaccgggga acatttaacg ggagaaataa cggcagagga actttgtttg
      121 tgtacgcttc cggtggaaat ggtgccggtg gcggtgattg cgccaacaca tcaaatcttc
      181 agggctatgt gaacggcgct ttcattggta tgaatgccag caataacccc tcctacggaa
      241 agacagcatt tatttcattt gccgtgccag tgggagcgtc atatcaaatc atctccagac
      301 caacacaaaa ttacgcctgt ggtaacggtg ttttctctgt gtatgcctat cagatgtgag
      361 tccctagctg gctttaacaa tgtagttgaa tgcaatattt ttgggacgca tttctgtggt
      421 actcccctgt gcccagtatg gcataccact atgatgccct ttcagccatc cgccacgttc
      481 gtgatagacg cctgattgat cggcctgata actcaggagt tgtctgttcc gatcaccatc
      541 aagtcccttc ccgttatccc agccacggat aaaaacaccg cgcagatcgg gcaatttaca
      601 gccaggataa gcacgggcaa gctgaggata ccgggaacag gaaaacgact ggccgttcat
      661 caacagatag ccttgtggag gagtggcaga tggccacggg atagggcttc ccacagggta
      721 ggattcaaag ccaacccaca caccggattg gcactggcct ccgtgccaat ccggtttgtg
      781 gcttggaggc aataaagtga actattctgc ctgtaaatgg tatgaagcga cctccgcaca
      841 ttttatcggt ggcagaagtg gaggttctat ttattacaag ccgattcagt gccctgccgg
      901 gtacgtcatg acaggtacaa gaatgtacgg catcggagat ggt
//
LOCUS       DBBGLQ010000409          932 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.416, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000409 DBBGLQ010000000
VERSION     DBBGLQ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.416"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..139)
                     /locus_tag="ACIYSB_005802"
     CDS             complement(<1..139)
                     /locus_tag="ACIYSB_005802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGC5432564.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFA
                     DE"
     gene            complement(229..597)
                     /locus_tag="ACIYSB_005803"
     CDS             complement(229..597)
                     /locus_tag="ACIYSB_005803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGC5432565.1"
                     /translation="MSDSRHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            complement(647..>932)
                     /locus_tag="ACIYSB_005804"
     CDS             complement(647..>932)
                     /locus_tag="ACIYSB_005804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HGC5432566.1"
                     /translation="VQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQIMTTDAE
                     AAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLRAG"
BASE COUNT          202 a          225 c          288 g          217 t
ORIGIN      
        1 gttcatcggc aaacggtgtg tcattcagtg tgaggccgta gtgctggtcc agcagtcggg
       61 acagcagtat ctgccagatg gtgacgggag acgggcagcg cgatgcctcc cgtacatgcg
      121 tgtcgggtaa tgttttcata ctgaagattt tcctgatatg cagagatgaa aaatggaaaa
      181 gtggcgtggt gaaaacacca ggccggagca gaaggttatt ctggagagtt actttttcgt
      241 ttcgggcgtc ggataaacag ccatataaac gtagccacaa ctgccgaggg tgtcggcttc
      301 gcaggtcagc ccttttgcat acagtgtcac ggtatgctga tggcggggat tcagttcacc
      361 gctggtgagc atgagttcca gttgtttcat cagcagcgga aaggcctggt ccaggtggta
      421 cgcatccgcg tcgctgaacc ggcctctgat atcggcgcgg tcggcaaggt aatgcaactg
      481 attgccctcc tgcaccagac gggcgccgaa acagggtgtc acggtgcagg gcagtcccca
      541 ccaggggcgg tcgttatggt cgtcgggata atttgtctca tggcgcgagt ctgacacgat
      601 aaaatcctta cagaaaatcg gtgaatgata gtttaacgat gacgggtcag ccggcgcgca
      661 gaccggcttc agtgaaggtg tattcgttga tgagaagagc ctcatcaacg gcggcgtctg
      721 aggtcagcca gtcgtactcg ttttccagtt gacggtacaa ccagaaggcc aggtcgcgca
      781 gggcttcgct gactgcggct tccgcatcgg ttgtcattat ctgaccagtc ggactgttcc
      841 gggtgacggt gatatccatg ctgtacggat ggcagtaacg tccccggtga ctgatttcag
      901 cctgtaactg ccagaagtta cgcttctgta cc
//
LOCUS       DBBGLQ010000410          916 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.417, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000410 DBBGLQ010000000
VERSION     DBBGLQ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.417"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..887
                     /locus_tag="ACIYSB_005805"
     CDS             <1..887
                     /locus_tag="ACIYSB_005805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGC5432567.1"
                     /translation="EKYYYIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIA
                     NTEFSYLALPENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTA
                     EKLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLD
                     GIDIIEIIYIKRPSKDLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGN
                     VPLNYNVHHKLSLDDGGTNDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWA
                     IPSGSIYPPMKNIMDHTK"
BASE COUNT          359 a          160 c          147 g          250 t
ORIGIN      
        1 tggaaaagta ttattatata caactaagag gcgctttttt atcggctatt catcaccaga
       61 tcattgaaaa ccaactggat acagaaacaa taactattag ttatgaattt atcctctgtc
      121 aacatcttat cgcaaatacc gagttcagct atttggcact ccctgaaaat tataaccgtt
      181 tgtttttacc aaattcaaaa aaccaaacaa ataatcgttt caaaacgtta aacagcaaag
      241 ctattggcag gctacttgct gctggtggcg tatacaatgg gaacattgaa ggattcagag
      301 atactgcgga aaaactgggt ggagatgcaa taaaaggcta tgatcaaata ctaaatgaaa
      361 aaacagcggg catagcgata gcaacagcat ctattctttt aacaaagcgt tctaatgttg
      421 atacatatac agaaataaat agttacttag gcaaacttag aggtcaacaa aaacttcttg
      481 atggtataga cataatagaa ataatataca ttaagagacc ttcaaaagac ttagctaact
      541 tacgaaagga gtttaataaa actgtaagaa aaaattttct tatcaaactt gcaaaaacct
      601 ccgaagcatc tggaagattc aacgccgaag accttttaag aatgagaaag ggcaatgttc
      661 ctctaaatta taatgttcac cataaactat ctctagatga tggtggtact aatgatttcg
      721 aaaatttagt attaatcgaa aacgaaccat atcataaagt ttttactaac atgcaatcac
      781 gaatagctaa gggaatatta gtaggtgaaa gcaaaatcac tccctgggcc attccatctg
      841 gctcaattta tcctcccatg aaaaatatta tggaccacac aaaatgatga ctaaaaccca
      901 aataaataaa ttaata
//
LOCUS       DBBGLQ010000411          913 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.418, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000411 DBBGLQ010000000
VERSION     DBBGLQ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.418"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..913
                     /locus_tag="ACIYSB_005806"
                     /pseudo
     CDS             1..913
                     /locus_tag="ACIYSB_005806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          218 a          232 c          276 g          187 t
ORIGIN      
        1 aaacgcagac ggcgtactac acaggaaaag atcgctattg ttcagcagag ttttgaaccg
       61 ggaatgacgg tctcccttgt tgcccggcaa cacggtgtgg cagccagcca gttatttctc
      121 tggcgcaagc aataccagga gggaagtctt actgctgtgg ccgcaggaga gcaggtcgtt
      181 cctgcctctg aacttgctgc tgccatgaag cagattaaag aactccagcg cctgctcggc
      241 aaaaaaacga tggaaaatga actccttaaa gaagccgttg aatacgggcg tgcaaaaaag
      301 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttcgtc agccgttgtc
      361 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg agccgatgac tggaaggacg
      421 gccgccgccg ccgtcacacg gatgatacgg atgtgcttcg ccgtatacac catgttatcg
      481 gagagctacc cacatatggt tatcgtcggg tatgggcgct gcttcgcaga caggccgaac
      541 ttgatggtat gcctgcgatc aatgccaaac gtatttaccg gatcatgcgc cagaatgcgc
      601 tgttgcttga gcgaaaaacc gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      661 ctgtgaaaga aagtaatcag cgatggtgct ctgacgggtt cgagttccgc tgtgataacg
      721 gagaaaaact gcgagtcacg ttcgcgctgg actgctgtga ccgtgaggca ctgcactggg
      781 cggtcactac gggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      841 aacgccgctt cggcaacgag cttccggcgt ctccagtaga atggctgacg gataatggtt
      901 catgctaccg ggc
//
LOCUS       DBBGLQ010000412          910 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.419, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000412 DBBGLQ010000000
VERSION     DBBGLQ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.419"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            260..>910
                     /locus_tag="ACIYSB_005807"
     CDS             260..>910
                     /locus_tag="ACIYSB_005807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432568.1"
                     /translation="MKFIMAKNVLAMAVTGALLCYSVGVMAEESFNVDGYDITLYDNG
                     AAIISKDGDIHQINTISGVNTGSIQPNESITRAILAAPDNIRHIVAQNLLTYPIYSDA
                     LKAENIRNIKAEDIKSIVDTKKSLEKIITPENKDDYISAVNAGGTAEDFIKISNKTSG
                     LSSLYKNVVEGQNFVLDSSGRITADGRADGNAQTIPVKEFVVELDNRTRNLGVEVKS"
BASE COUNT          295 a          145 c          197 g          273 t
ORIGIN      
        1 ctgattcaca attgagtctg tgatgaatga ctgatgtagg aaacggttat tctgacagct
       61 atatctgaat gctctgctga catatggtga caggaacgct catattgttt tatcaggggg
      121 catttatcct ataattccat gttaactgta ttgattgtta aaatcaatac agtggtgact
      181 ctgaaaggta tcctggaaaa gaatctcgcc tgcttattca gggggggtac agtttaatct
      241 tcttaacagg atataccata tgaaatttat aatggcaaag aatgtattgg caatggcggt
      301 taccggtgcg ctgttatgtt actctgtggg ggttatggca gaagagagtt ttaatgtaga
      361 tggatatgat attactctgt atgataatgg tgcagcaata atatcaaaag atggtgatat
      421 tcatcaaatt aatactatta gtggcgttaa tacagggtct attcaaccga atgaatctat
      481 tacgcgtgct atattagcag ctcctgataa tattaggcat attgttgctc agaatttgct
      541 aacttacccc atctactcag atgcattaaa agccgaaaac atcagaaata taaaagcaga
      601 agatataaag tccattgttg atacaaaaaa atcactagaa aaaataatca caccagaaaa
      661 caaggatgat tatatctccg ctgttaatgc tggtggtacc gctgaggatt ttattaaaat
      721 tagtaataaa acatcgggat tgtcatcatt gtataaaaat gtagttgaag gccagaattt
      781 tgttctggat tcatcaggcc gaatcactgc tgacggaagg gctgacggaa atgctcagac
      841 aatccctgtt aaagaattcg tcgtggagct ggataatcgt acccgcaacc ttggggttga
      901 agtgaaatca
//
LOCUS       DBBGLQ010000413          893 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.420, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000413 DBBGLQ010000000
VERSION     DBBGLQ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.420"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..589)
                     /gene="fimE"
                     /locus_tag="ACIYSB_005808"
     CDS             complement(<1..589)
                     /gene="fimE"
                     /locus_tag="ACIYSB_005808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGC5432569.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKRE"
BASE COUNT          282 a          194 c          170 g          247 t
ORIGIN      
        1 cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg gcaaaacgag
       61 cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgatgcccg agataatcct
      121 gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca tgccttaaca
      181 tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga ataatgcgat
      241 aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg tcggtccggt
      301 cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca cgctcatcaa
      361 aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt attcgacctt
      421 cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc atcccatgcc
      481 gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa caaaccgcct
      541 gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca atagtagtac
      601 tcctgactga gatatatttg aatgaatacc tataggaaac ctcaatcggt caaaattagc
      661 ccaatagata aaaagataac cacacacgaa gtgatgtggc tatcagtcaa ttacattaaa
      721 tatcatacaa aaataaaata tcactgatgt gataacatct attttcattg atttgattca
      781 atataacaat agattgcagt ggattaactt ccagttgctt aaaaaataaa gaaaaaatgt
      841 gaaaacagat gaatttaagt gatgtgtaaa tttatgaatg attttgttta ttt
//
LOCUS       DBBGLQ010000414          889 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.421, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000414 DBBGLQ010000000
VERSION     DBBGLQ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.421"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>889
                     /locus_tag="ACIYSB_005809"
     CDS             <1..>889
                     /locus_tag="ACIYSB_005809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGC5432570.1"
                     /translation="MNDAASGSKSGPYLTPAGETGGSVGRLGNEGDTYVETVFEHKKT
                     LESGATTRFKVMLADGQKTYNDWSANSSDLNVRQVVAELGNLPDFTGPLKHSTVWAGK
                     RFDRDNFDIHWLDSDVVFLGGTGGGIYDVKWNDTLRSNFSLYGRNFGEMEDIDNNVQN
                     YILTMNHFAGPFQLMVSGMRAKDNDDRRDNNGDLIHASAASHGVHVLAGLHNDSFWGL
                     REGTAKIALLYGHGLGAEVKGIGSDGALRSDADTWRLAAYGTTPLGNGWHIAPSVMAQ
                     GSKDRYARGDSYEWVTLNTR"
BASE COUNT          205 a          219 c          280 g          185 t
ORIGIN      
        1 atgaacgatg ccgcatccgg cagcaaaagt ggtccgtatc tgacgcctgc cggagagacc
       61 ggtggctcgg tcgggcgcct cgggaatgag ggagacactt atgtggaaac cgtgtttgag
      121 cacaaaaaga cgctcgaaag cggggccacc acccggttta aggtgatgct ggcggatggt
      181 cagaaaacct ataacgactg gtcggcgaac tccagtgacc ttaacgtccg ccaggtggtg
      241 gctgaactgg gaaatctgcc ggacttcacc gggccgctga agcacagtac ggtatgggcg
      301 ggtaaacgtt tcgatcgtga caactttgat atccactggc tggactcgga cgtggttttc
      361 ctcggcggaa ccgggggcgg tatctatgac gtgaaatgga acgacactct gcgcagtaac
      421 ttctccctgt atggacgcaa tttcggggag atggaggata tcgataacaa cgtccagaac
      481 tatatcctta ccatgaacca ctttgccggg ccgttccagc ttatggtcag cggtatgcgg
      541 gcaaaggata atgatgaccg cagggacaat aatggcgacc ttatccatgc cagtgccgcc
      601 agccacggtg tgcatgtgtt ggccggactt cataatgaca gtttctgggg attacgtgag
      661 ggaacggcga aaatagcact tctgtacggg catggtctgg gggcggaagt gaaggggatt
      721 ggctctgacg gtgcactacg cagtgatgcg gatacttggc gtcttgctgc atatggtacc
      781 accccgctcg gaaacggctg gcatatcgcc ccgtcagtca tggcgcaggg cagtaaggat
      841 cgctatgccc gtggagacag ttatgaatgg gtgaccctga acacccgct
//
LOCUS       DBBGLQ010000415          887 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.422, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000415 DBBGLQ010000000
VERSION     DBBGLQ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.422"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..381)
                     /locus_tag="ACIYSB_005810"
     CDS             complement(<1..381)
                     /locus_tag="ACIYSB_005810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432571.1"
                     /translation="MTNIANMESLYFYCNKIWLIHRRIRGKKLSGMFLRGVVKPLAGL
                     PLQQRRNIPSFISTPDAVKTWLMQTGLLISNQSFLIFSRTQNKTNQAIPYTPPLKLRG
                     FFYHINKLLAQLLKKLQIFTVVSGF"
BASE COUNT          241 a          160 c          185 g          301 t
ORIGIN      
        1 gaagcccgac actacagtaa agatttgcaa tttttttaaa agctgtgcaa gtaatttatt
       61 tatatggtaa aaaaaacccc tcaattttag agggggagtg taaggaattg cctgatttgt
      121 tttattttgt gtacgcgaaa aaatgagaaa gctttgattc gatatcagca gtcctgtctg
      181 catcagccag gtctttacag cgtcaggagt actgataaag gatggaatgt tcctgcgttg
      241 ctgcaaaggc aatccggcta gaggttttac cacaccacgc agaaacattc cggataattt
      301 tttaccccgg atccgcctgt ggatcaacca gattttgttg cagtaaaaat acagtgattc
      361 catatttgca atatttgtca caaaaatagc gtaaacgcgt taaatttttg ttgccgggtg
      421 tttttgttac tgattatgat ttccctgtac tcctgaggag tacagtcatg tgcatactaa
      481 aatccgtgtt ttcgataaaa ttaaggtcgt gattctgtat cacgaccttt tccttgcctg
      541 aaaggatatt ttacgctaga agagttatga gcgcgtctct ggtgtttgta gcagggataa
      601 tatctaatat ggtgcaccgg aaacgcgctc tccctttgta tgcacaaagt cctttaattt
      661 cttctgcctg attttcccga catatatcct cgtgtaaaaa acagagtgtg tttctgtcta
      721 caatgttcat tgcttctgtt ccgtgttcca gaacacgcct ctttttgaga agaatgtcag
      781 tctgatatag agagagctgg ttgtatctgt gtcagagttt tgttattttt ttgtatgctc
      841 agttattgat ggataataat attaaatgaa tcagtaagtt ccttact
//
LOCUS       DBBGLQ010000416          884 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.423, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000416 DBBGLQ010000000
VERSION     DBBGLQ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.423"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..875
                     /locus_tag="ACIYSB_005811"
     CDS             <1..875
                     /locus_tag="ACIYSB_005811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HGC5432572.1"
                     /translation="PEMKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTR
                     QQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASR
                     KFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRA
                     VIGWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRG
                     SMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWC
                     GGLSPEQFENKNLA"
BASE COUNT          205 a          224 c          265 g          190 t
ORIGIN      
        1 cgcctgaaat gaagtatgtc tttattgaaa aacatcaggc tgagttcagc atcaaagcaa
       61 tgtgccgcgt gctccgggtg gcccgcagcg gctggtatac gtggtgtcag cggcggacaa
      121 ggataagcac gcgtcagcag ttccgccaac actgcgacag cgttgtcctc gcggctttta
      181 cccggtcaaa acagcgttac ggtgccccac gcctgacgga tgaactgcgt gctcagggtt
      241 acccctttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcagggactg agggcaaagg
      301 cctcccggaa gttcagcccg gtcagctacc gcgcacacgg cctgcctgtg tcagaaaatc
      361 tgttggagca ggatttttac gccagtggcc cgaaccagaa gtgggcagga gacatcacgt
      421 acttacgtac agatgaaggc tggctgtatc tggcagtggt cattgacctg tggtcacgtg
      481 ccgttattgg ctggtcaatg tcgccacgca tgacggcgca actggcctgc gatgccctgc
      541 agatggcgct gtggcggcgt aagaggcccc ggaacgttat cgttcacacg gaccgtggag
      601 gccagtactg ttcagcagat tatcaggcgc aactgaagcg gcataatctg cgtggaagta
      661 tgagcgcaaa aggttgctgc tacgataatg cctgcgtgga aagcttcttt cattcgctga
      721 aagtggaatg tatccatgga gaacacttta tcagccggga aataatgcgg gcaacggtgt
      781 ttaattatat cgaatgtgat tacaatcggt ggcggcggca cagttggtgt ggcggcctca
      841 gtccggaaca atttgaaaac aagaacctcg cttaggcctg tgtc
//
LOCUS       DBBGLQ010000417          879 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.424, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000417 DBBGLQ010000000
VERSION     DBBGLQ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.424"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..720
                     /locus_tag="ACIYSB_005812"
     CDS             <1..720
                     /locus_tag="ACIYSB_005812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432573.1"
                     /translation="ATNSTSEVLAATPKAVKAAYDLANGKQAADATLTALAALATAAD
                     KLPYFTGVDRAALTALTSVGRAILSKPSIQGVLNYLGLGEGSALPVGVPVPWPSATPP
                     TGWLKCNGAAFSSEKYPNLAKAYPTLKLPDLRGEFIRGWDDGRGVDAGRALLSIQTGM
                     LEKHRHIVVANDGYDTKDEWELATIFKKTYTQGRGLDASNTGGNLIPSPTLHSRGSIG
                     NTGGSETRPRNIAFNYIVRAA"
     gene            731..>879
                     /locus_tag="ACIYSB_005813"
     CDS             731..>879
                     /locus_tag="ACIYSB_005813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432574.1"
                     /translation="MGEGSALPVGVPVPWPSATPPTGWLKCNGAAFSSEMYPRLAMAY
                     PTNKLP"
BASE COUNT          228 a          210 c          235 g          206 t
ORIGIN      
        1 gccactaaca gtacgtctga agtgctggcc gccacaccga aagctgtcaa ggctgcatat
       61 gatttggcta atggaaagca agcggcagac gctacgctca ctgctctagc ggcactagct
      121 accgcagcag ataaactccc ttatttcaca ggggttgatc gtgccgcgtt aactgcactg
      181 acaagtgttg gacgtgcaat tcttagtaag cccagtattc agggtgttct taattacctt
      241 ggtttagggg aaggctcggc gctgcccgtt ggcgtgcctg ttccgtggcc ctccgccaca
      301 ccaccaacgg ggtggctgaa atgtaacgga gcagcatttt cttctgaaaa gtacccaaat
      361 ctggcaaagg cttacccaac gttaaaatta cctgatttac gcggtgagtt tatccgtggt
      421 tgggatgacg ggcgcggggt ggatgcggga cgtgctttat tgagcattca gacaggaatg
      481 ctggaaaagc accgacatat tgttgtagcc aatgatggtt acgacacaaa agatgaatgg
      541 gagctggcga caattttcaa aaaaacatac acacaaggac gggggcttga tgcctcaaat
      601 acaggaggaa atttgattcc atcaccgaca cttcattctc gagggagtat tggtaatact
      661 ggcgggagtg aaacacgtcc acgaaatatc gcatttaatt acatcgtaag ggcggcataa
      721 aaacgttggt ttgggggaag gctctgcact gcccgttggt gtgcccgttc cgtggccctc
      781 agccacgccg ccaacggggt ggctaaaatg taacggagca gcattttctt ctgaaatgta
      841 tcccagactg gcaatggctt accccaccaa taaattacc
//
LOCUS       DBBGLQ010000418          857 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.425, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000418 DBBGLQ010000000
VERSION     DBBGLQ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.425"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            122..>857
                     /locus_tag="ACIYSB_005814"
     CDS             122..>857
                     /locus_tag="ACIYSB_005814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGC5432575.1"
                     /translation="MINFTIFTPTYNRADTLERCYRSILLQKRIDIEWLIIDDGSTDN
                     TKFLVEKFMAEGKIRIVYIYQNNAGKQVAWNVAIQKANGQYFIGLDSDDALYEDVLSV
                     LFPKINEINADSSIVGLRCLSQKVPKGSNRIKDFSSGHDSIHYWYDEFASGISQERID
                     ILKTKIIKEYPYPVQDGVKFIPEVWFYSTISKKYKFYYVNKVVRVFYDDLSTNRLSRS
                     SFKKHFKGHLIARKAMLHNIPIIYFLK"
BASE COUNT          321 a          109 c          148 g          279 t
ORIGIN      
        1 tattatgtat ggtatggcct gttatcagaa atgaacatgg caatgatatt taatataaat
       61 tacgttataa tgactgctat aacctttctt tttgttaaaa aaggactaaa agttagagac
      121 tatgattaac tttacaattt ttactcccac atataataga gcggacacat tagaaagatg
      181 ttatcgaagc atcctacttc agaaacgaat agacatagaa tggctaatta ttgatgacgg
      241 ttctactgat aatactaagt ttttagtaga aaaatttatg gctgaaggga agattagaat
      301 agtatatatt tatcagaaca atgcaggaaa acaagttgct tggaacgtcg cgatacaaaa
      361 ggctaatggt cagtatttta taggacttga ttctgatgat gctttatatg aggatgtatt
      421 atcagttcta tttccaaaaa taaatgaaat aaatgcagat tcaagtattg tcggactacg
      481 ttgtctgtca caaaaagttc caaagggaag caatagaatt aaagattttt catcaggaca
      541 tgattcaata cattactggt atgatgaatt tgctagtggt atttcacaag aaagaattga
      601 tatattgaaa acaaagatca ttaaagaata tccgtatcct gttcaagatg gtgttaaatt
      661 tataccagag gtatggtttt acagtacgat atcaaaaaaa tacaaatttt actatgtaaa
      721 taaagttgtt agagtatttt atgatgacct tagtactaat aggttaagcc gaagttcttt
      781 caaaaagcat ttcaaaggcc atttaattgc acgtaaagct atgttgcaca atatacctat
      841 aatatacttt ctaaaaa
//
LOCUS       DBBGLQ010000419          847 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.426, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000419 DBBGLQ010000000
VERSION     DBBGLQ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.426"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            44..568
                     /gene="sodC"
                     /locus_tag="ACIYSB_005815"
     CDS             44..568
                     /gene="sodC"
                     /locus_tag="ACIYSB_005815"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGC5432576.1"
                     /translation="MKRKLIAFVILATSSCGYAATKDVTMNLVDSDGKERNIGKITIQ
                     KTPYGLLFTPDLHSLPAGIHGFHVHENASCAPALKDGKTVAALSAGGHFDPEKTGKHL
                     GPWSPDGHLGDLPALYVTHDGKATYPVLAPRLSELKELDGRSLMLHAGGDNHDDHPEP
                     LGGGGARIACGIIP"
BASE COUNT          219 a          210 c          173 g          245 t
ORIGIN      
        1 caacagttat tgagtaaatc tttaacctgt taacagaggg tttatgaaac gtaaacttat
       61 tgcctttgtg attctggcta catcatcctg tggatatgca gccaccaaag atgtaacaat
      121 gaatcttgtt gatagtgacg gaaaagagcg aaatattggt aaaataacca tccagaagac
      181 accctacggg ctgctgttca cccctgatct tcactcctta cccgccggca ttcatggttt
      241 tcatgtacat gaaaacgcaa gttgtgctcc tgccctgaaa gatggaaaaa ctgtcgctgc
      301 tttatctgca ggtggtcact ttgacccgga aaaaacaggg aagcatctcg gtccatggtc
      361 tcctgatggc cacctgggtg atctccctgc gctttatgtc actcatgacg gaaaagcaac
      421 ctacccggtg cttgcgccaa gactatctga attaaaggag ctcgatggcc gttcgctaat
      481 gctccacgca ggaggtgata atcatgatga ccatcctgaa cctctaggtg gcggtggtgc
      541 aagaattgcc tgtggtatca tcccataatc aatcaggtaa gggatatcct cccttaccct
      601 taatcttccc tggctgcatg tgcatatctt tcagttactg tgagatacct gtattacggt
      661 gtctttttag ctcacagaaa tatcagacag cccgtttcag tgttttatta ccttaatctt
      721 tttacggcat tcttcagctc ttgcgcatgc tattttttca cctgtttcat aacattgctg
      781 gcagcctaat ccaaatttca tcgccatatc ataaccttcc cgaaaacctc ggaaccacgc
      841 gttacga
//
LOCUS       DBBGLQ010000420          840 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.427, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000420 DBBGLQ010000000
VERSION     DBBGLQ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.427"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            295..>840
                     /locus_tag="ACIYSB_005816"
     CDS             295..>840
                     /locus_tag="ACIYSB_005816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HGC5432577.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSAD"
BASE COUNT          210 a          172 c          186 g          272 t
ORIGIN      
        1 acttatatgt tcgctgattt taagcaccga gactatccgg tcttttacca aataatgaag
       61 aataatccct ccctggaaga taattgtaaa aaacaacagc ataagaatta atcttaggat
      121 aaatttttat ttatcatggc cttttgaacg ctgaaaagta gcgctctgca aagtaaaccc
      181 cgctatagtt atgctatgca tttgtcttta atgatatctc gtcattatat taacaggatg
      241 aaattatcat taatgcatat ttcaatatta gcagggatac ctgaggagtt taaagtgaac
      301 atatatatcg ggtggctttt caaattaatc cctttgatta tgggcttaat ttgtatcgcg
      361 ttaggtggct ttgtgctgga aagttcaggg caaagcgagt atttcgtcgc gggtcatgtg
      421 ctgatttctc tggcggccat atgcctggca ttattcacta ccgcatttat tatcatttcg
      481 cagctcacgc gcggcgttaa tacgttttac aatacattgt tccccattat tggctatgcg
      541 gggtcaatta tcaccatgat atggggttgg gcactgttag caggcaatga tgtgatggca
      601 gacgagtttg tcgccggcca tgttattttc ggcgttggta tgattgccgc ctgtgtatcg
      661 acggtggcag cgtcatccgg tcactttctg ctcattccca aaaatgcagc ggggagcaag
      721 agcgacggaa caccggtaca ggcttattct tcattaatcg gtaactgcct cattgccgtt
      781 cccgttttac tcaccctgct cggtttcatt tggtctatta cgctgttacg tagtgctgac
//
LOCUS       DBBGLQ010000421          832 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.428, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000421 DBBGLQ010000000
VERSION     DBBGLQ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.428"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..832
                     /locus_tag="ACIYSB_005817"
                     /pseudo
     CDS             1..832
                     /locus_tag="ACIYSB_005817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
BASE COUNT          220 a          210 c          239 g          163 t
ORIGIN      
        1 acgtgatgag cggcagggaa aaacaccaaa agcctccccc attaccccgg aacaaattga
       61 aatccgtgag ctcaggaaaa agctacaacg tattgaaatg gaaaatgaaa tattaaaaaa
      121 ggctaccgcg ctcttgatgt cagactccct gaacagttct cgataatcgg gaaactcagg
      181 gcgcgttatc ctgtggccac tctctgccat gtgttcgggg tccatcgcag cagctacaaa
      241 tactggaaaa accgtcctga aaagccagac ggcagacggg ctgtattacg cagccaggta
      301 cttgaactgc atggcatcag ccacggctct gccggagcaa gaagcatcgc cacaatggca
      361 acccagagag gctaccagat ggggcgctgg cttgctggca gactcatgaa agagctgggg
      421 ctggtcagct gtcagcagcc gacttaccgg tataaacgtg gtggtcatga acatgttgct
      481 atccctaact accttgaacg gcagttcgcc gtgaccgagc caaatcaggt gtggtgcggt
      541 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
      601 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gcttaccatg
      661 aaagcactgg aaatggcatg ggaaacccgt ggtaagcccg tcggggtgat gttccacagc
      721 gatcaaggca gtcattatac gagcaggcag ttccggcagt tactgtggag ataccggatc
      781 aggcagagta tgagtcggcg tggaaactgc tgggataaca gcccaatgga gc
//
LOCUS       DBBGLQ010000422          829 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.429, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000422 DBBGLQ010000000
VERSION     DBBGLQ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.429"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..98)
                     /locus_tag="ACIYSB_005818"
     CDS             complement(<1..98)
                     /locus_tag="ACIYSB_005818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432578.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSN"
     gene            complement(98..517)
                     /gene="umuD"
                     /locus_tag="ACIYSB_005819"
     CDS             complement(98..517)
                     /gene="umuD"
                     /locus_tag="ACIYSB_005819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGC5432579.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          254 a          195 c          168 g          212 t
ORIGIN      
        1 ttattcgata gcacaaccac cggtttaccc cataaatcag ggcgaaacac cgtctcacag
       61 ctggcataaa acgcgtttac atcacagagg gcaaacatca gcgcatcgcc ttaacgacgt
      121 ggatcaccac accaaagaca tccagcgtat cttcactact gatggtaatg ggcgagtacg
      181 cgctgttcat gggaataagc tgtaccgtcg ggcgtagttg caattttttc accgtaaact
      241 cgccgtcaac agcagcgatg acaatatcac catggctggc ggtaatagcg ctatcgacaa
      301 tcagtaaatc accgtcacta attccaccat caatcataga atcaccactt gctttgacga
      361 agtaagtcgc gctgggatgc tggatcaaca gttgattcag atcgatgcgc tgttcaacgt
      421 aatctgctgc cggtgaagga aagccacact gaacaagatc gctaaatagc ggaaaagtca
      481 caatttcgcg gagatccgca ggcttgataa acaacataat aatctgcctg aagttatact
      541 gtttttatat acagtagtct gttcttgcca gcagatcaat actgattcag gctatcaata
      601 tttgtcgctg cataggctgc tgatttttcg ttctcttatc ttgtgctcac gtggccttct
      661 ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc ggctaaaaaa tagaatagaa
      721 gcattcgcca taatgacatt aaacattgtt tgatatttat catattaata gaaataaaga
      781 cattgacgca tcccgcccgg ctaactatga attagatgaa gtaaaattt
//
LOCUS       DBBGLQ010000423          828 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.430, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000423 DBBGLQ010000000
VERSION     DBBGLQ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.430"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>828
                     /locus_tag="ACIYSB_005820"
     CDS             <1..>828
                     /locus_tag="ACIYSB_005820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter barrel
                     domain-containing protein"
                     /protein_id="HGC5432580.1"
                     /translation="SDEPSDYAGPGDVAMSFTASGSDYAMKNNVFLSNSTLMGDVAFT
                     STWNSNFDPNGHDSNGDGVKDTNGGWTDDSLNVDELNLTLDNGSKWVGQAIYNVAETS
                     AMYDVATNSLTPDATYENNDWKRVVDDKVFQSGVFNVALNNGSEWDTTGRSIVDTLTV
                     NNGSQVNVSESKLTSDTIDLTNGSSLNIGEDGYVDTDHLTINSYSTVALTESTGWGAD
                     YNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVVSGVFDIHSSD
                     "
BASE COUNT          226 a          166 c          200 g          236 t
ORIGIN      
        1 tctgatgaac cgagtgatta tgctggcccg ggtgatgttg caatgtcctt tactgcttca
       61 ggttccgact atgcgatgaa gaacaatgta ttcctcagca attcaacgct gatgggtgat
      121 gttgccttta ccagcacctg gaatagtaat tttgatccga atggtcatga ttccaacggt
      181 gacggggtga aagataccaa cgggggttgg actgatgata gcctcaacgt tgatgaacta
      241 aatctcactc tcgataacgg aagcaagtgg gttggtcagg caatttataa cgttgctgaa
      301 acgtcagcaa tgtatgatgt tgctacaaac agccttactc ctgatgcaac atatgaaaac
      361 aatgactgga aacgtgttgt tgatgacaag gtcttccaga gcggtgtatt taacgtagcg
      421 ttgaataacg gttctgaatg ggatactaca ggtcgttcca tcgttgatac cttgacagtt
      481 aataatggtt ctcaggttaa tgtttcggaa tctaaattaa cttcagatac tatcgattta
      541 actaacggtt cttcgctgaa cattggtgaa gatggctacg ttgataccga tcatctgact
      601 attaactcct acagtactgt tgcgttgacc gaatctactg ggtggggggc tgattacaac
      661 ctgtacgcca atactatcac cgtaactaac ggcggtgtat tggatgtgaa cgttgatcag
      721 ttcgatactg aagctttccg tactgacaaa ctggaactga ccagcggcaa catcgctgac
      781 cataacggta acgtagtatc tggtgtgttc gatatccata gcagcgat
//
LOCUS       DBBGLQ010000424          827 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.431, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000424 DBBGLQ010000000
VERSION     DBBGLQ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.431"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            350..>827
                     /locus_tag="ACIYSB_005821"
     CDS             350..>827
                     /locus_tag="ACIYSB_005821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein"
                     /protein_id="HGC5432581.1"
                     /translation="MLKRRDAFLKKSALAVSVALLLSSQAQAQAQAQAHKTLTELSTG
                     IIWIDNGTQSLERASVIDRNGNANGDASVTGKNFAVGSDAKIWDADKSMAVGNNTAVF
                     NADNSVALGYGSQVDRESNVLSVGAGPSGYGFSVDGAPETRRIINVSDGVKDSDAAT"
BASE COUNT          231 a          142 c          192 g          262 t
ORIGIN      
        1 gtacgtctgc attcctcgtt ttgaacagaa ttctcaccag acatcgtgaa tttctcattc
       61 aatcatcaga gatattaccc aggattttta ttgttttgtg atagcagtac tttaaatgtt
      121 cagatgctaa aataagcgcg cttattataa ttatagttat gatgttttat ctgtatgtat
      181 tttaatgatt ttcgcgtcat tgcttttctg ctgcttaaat tgagggcgca tattatacac
      241 aataatattc ctggtattat taagttaata agagaggatt cttatcactg tatttttgct
      301 ccgcactacc gttctgactg gtgaagtaat aatacaggag aataacagaa tgcttaaaag
      361 acgtgatgct tttttaaaga aaagcgcgct ggcagtgagt gtggccctgc tgctctcatc
      421 tcaggctcag gctcaggctc aggctcaggc tcataaaaca cttactgagc tatcgacggg
      481 tattatctgg attgataatg gcactcagtc acttgaaaga gcaagtgtga ttgacagaaa
      541 tggaaatgct aacggtgatg ctagtgttac tggaaaaaac tttgctgttg gttccgatgc
      601 aaaaatttgg gatgcggata aatccatggc tgtgggaaat aatacagctg tttttaatgc
      661 agacaacagt gtcgctctgg ggtatggctc tcaggtggat agagaaagca atgtactttc
      721 tgtaggggcc ggtccttcag gatatggatt ttcagttgat ggtgcaccgg aaacccgccg
      781 gattataaat gtttcagatg gtgttaagga tagtgatgcg gccacaa
//
LOCUS       DBBGLQ010000425          798 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.432, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000425 DBBGLQ010000000
VERSION     DBBGLQ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.432"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(176..409)
                     /locus_tag="ACIYSB_005822"
     CDS             complement(176..409)
                     /locus_tag="ACIYSB_005822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGC5432582.1"
                     /translation="MLTTTSHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(433..>798)
                     /locus_tag="ACIYSB_005823"
     CDS             complement(433..>798)
                     /locus_tag="ACIYSB_005823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432583.1"
                     /translation="TRHPEKPEYHVVLCVRQEYFDGPELDRLILDAWSNVLGFASPGE
                     AAPYQKQITRDVVLDSRSPDCEDILKELIWAFSDFARDRRGVRDPEARYLAGNPWYPV
                     AGQLRKHSSADSPAFAQPS"
BASE COUNT          177 a          210 c          221 g          190 t
ORIGIN      
        1 gacgaaggga atgacgaatc agtcaggaaa tgatgacaaa cagttggctg gccagagggt
       61 gccagcagaa agatgagcgg gagttgtcag cgccagcacc agggacaggg ctgatttcca
      121 caataccagc gccggcattt cgcacattgc cagcgtactg gcattacaac acgtttcatg
      181 ctgctgctcc tcgtgattcc tcaattcgct gctgcatcca ctgctccact tcacttttgt
      241 accagcggct gctgcgcccc agtttgatgg gtttagggaa atgaccttca ctgatcagct
      301 tgtaaaacca tttatcggtc ataccggtga aactggtgat gtagttcatg tcgatcaggg
      361 gatcgtcggc acgcagcaat acgctgtcgt ggcttgttgt ggtcaacatg gtcagaatgt
      421 cctttgtgag atttatgaag gttgtgcaaa tgcgggggaa tctgctgaac tgtgctttct
      481 gagctggcct gctacgggat accagggatt accggcgagg taacgggctt ccggatcgcg
      541 cacaccacga cgatcgcggg cgaaatcact gaacgcccat atcaactctt tgaggatgtc
      601 ttcgcagtcc ggtgaacgac tgtccagcac cacatcccgt gttatctgtt tctggtacgg
      661 tgccgcttca cccggtgacg cgaagcccag tacgttgctc caggcatcca gaatcagccg
      721 gtccagttca gggccatcga aatactcctg tctgacgcac agcaccacgt ggtattccgg
      781 cttttcagga tgacgggt
//
LOCUS       DBBGLQ010000426          796 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.433, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000426 DBBGLQ010000000
VERSION     DBBGLQ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.433"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..329
                     /locus_tag="ACIYSB_005824"
     CDS             <1..329
                     /locus_tag="ACIYSB_005824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGC5432584.1"
                     /translation="SAERTPPSAALTGIRCSTAHITETDNAWLYSLSNQTNDTGESEW
                     IHFTGSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLIRRYGVSLIHLDAGAECLQD
                     LPTFNW"
     gene            349..546
                     /locus_tag="ACIYSB_005825"
     CDS             349..546
                     /locus_tag="ACIYSB_005825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGC5432585.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREGIRTLRQLQLLHQNR"
     gene            631..774
                     /locus_tag="ACIYSB_005826"
                     /pseudo
     CDS             631..774
                     /locus_tag="ACIYSB_005826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          216 a          209 c          189 g          182 t
ORIGIN      
        1 catcagcaga gcgcacgcca ccatctgcgg cccttaccgg catacgctgc agtaccgcac
       61 atatcacgga aacggacaat gcctggttgt actcgctgtc aaatcagacg aatgacaccg
      121 gcgaatcaga atggattcat ttcacaggta gcggatatct gttacgtacc gatgcgtggt
      181 catacccggt tctgcggctt aaacgcctgg ggctgtccag aacgttccgc cgtctggttg
      241 tcacactcat ccggcgttat ggcgtcagtc tcattcatct ggatgccggt gctgaatgtc
      301 tgcaggattt acccactttc aactggtaac caggacaaaa gacacaggat gaaatcatta
      361 accacagaaa cggcgctgga tattctgatt gtgtggctgc aggacaatat cgactgcgaa
      421 tcgggaatta tctttgacaa cgatgaggat aaaacggatt cggcagcact gttgccctgt
      481 atcgaacagg ccagggaggg tatccgtacc ctgcgccaac tgcagcttct gcaccagaac
      541 cgataaatat tactcaccat acttcattcc actgacgcca gcctgaacag ggctggcgtt
      601 ttcatttatc tgcaaaaagg aatatcaatt atgtctgaaa tcacagcctc ccgtccggaa
      661 gtggtcaacg gggacattat ctgctccacc tcagtcagcc acattctggc tgtacaaaag
      721 agtacacagc tgcaaatcga cacacttatc cggcatcaag tgagcgatat cagttaacag
      781 atatagatgg agaaga
//
LOCUS       DBBGLQ010000427          782 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.434, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000427 DBBGLQ010000000
VERSION     DBBGLQ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.434"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..>782
                     /locus_tag="ACIYSB_005827"
     CDS             1..>782
                     /locus_tag="ACIYSB_005827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HGC5432586.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYS
                     GREDDRQVPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVM"
BASE COUNT          200 a          197 c          233 g          152 t
ORIGIN      
        1 atgagtcaga aatacctcat tcgcatcgca gagctggaaa ggttgctctc tgagcaggct
       61 gaagccctcc gtcagaaaga ccaacaactg agtctggttg aagagacgga ggccttcctg
      121 cgttctgcgc tggcacgtgc cgaagaaaag atcgaagaag atgaacggga aatagaacat
      181 ctgcgggctc agatagaaaa actgcgccgg atgctgttcg gtacccgttc tgaaaaactg
      241 cgtcgtgaag ttgaacaggc tgaggccctg ctgaagcaac gcgaacagga cagcgatcgt
      301 tacagtgggc gagaggatga ccggcaggtt ccccgccagt tgcgacagtc acgccatcgt
      361 cgtccgttac cggagcatct gccccgcgaa ataaaccgcc tggagcctga agaaagctgt
      421 tgcccggagt gtggcggtga gctggattat ctgggggaag tcagtgcaga acaactggaa
      481 ctggtgagca gcgccctgaa agtgatccgc acagaacggg taaaaaaagc ctgtacaaaa
      541 tgtgactgta tcgttgaagc accggcacca tcccgtccga tagagcgtgg tatcgcgggc
      601 ccggggttac ttgcccgcgt gttaacggga aaatactgcg aacacctgcc actgtatcgt
      661 cagagtgaaa tctttgcccg ccagggtgtc gaactgagcc gggccttact ctccaactgg
      721 gttgacgcgt gctgccagtt aatgacaccg gtgaatgatg ccctgtaccg ttatgtaatg
      781 aa
//
LOCUS       DBBGLQ010000428          782 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.435, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000428 DBBGLQ010000000
VERSION     DBBGLQ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.435"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..384
                     /locus_tag="ACIYSB_005828"
     CDS             <1..384
                     /locus_tag="ACIYSB_005828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGC5432587.1"
                     /translation="AVNIDLGLDDGALDASFTMGGPTRELFLKYGGTIDGTLLRFVGE
                     YYNDEEQSDLYEIELRGRVTEIDTGEAKQGEVTSHTYAVRSTYYKLSINDRPVLEIDL
                     LNHIERKDGKNVFPDRVRSAIGLGG"
     gene            435..>782
                     /locus_tag="ACIYSB_005829"
     CDS             435..>782
                     /locus_tag="ACIYSB_005829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432588.1"
                     /translation="MKENPVVAPVTDGADAADVTSERGVTLKTPLVLGNKKITYVEIT
                     PAVQQAGSLRGLSLLDVLNMKTDAMFELLPRVTSPRLDEVLLKRMGSADFIQLCGVAV
                     NFLAGQDSGGKSGA"
BASE COUNT          162 a          197 c          250 g          173 t
ORIGIN      
        1 gcggtgaata tcgaccttgg tctggatgac ggcgcgctgg atgcgagttt caccatgggc
       61 gggccgaccc gcgaactgtt ccttaagtac ggcggcacga tcgacggtac gctgctgcgc
      121 tttgtcggtg agtactacaa cgatgaagag cagagcgacc tgtatgaaat tgagcttcgc
      181 ggccgcgtga cggaaattga tacgggggag gcaaaacagg gtgaggtcac gtctcacacc
      241 tatgcggtca ggagtaccta ctacaaactc agcattaacg accgtccggt gctggagatt
      301 gatttactga accacatcga acgcaaggac ggcaaaaacg tcttcccgga ccgcgtgcgc
      361 tcggcgattg gtcttggtgg ttaatgtttc ccacggcggc gcgcggcgtc gccccctgat
      421 ttgaggtttt atgcatgaaa gaaaatccgg ttgttgcccc tgttactgac ggtgctgatg
      481 cggcggacgt gaccagtgag cgcggcgtga cgctgaaaac gccccttgtg ctcggtaaca
      541 aaaaaatcac gtatgtggaa atcacgcctg ctgtacagca ggccggttca ctgcgcgggc
      601 tgtcactgct tgacgtgctg aacatgaaaa cggacgcgat gtttgagtta ctcccgcgcg
      661 tcacttcgcc gcgactggac gaggttctgc ttaagcgcat ggggtccgct gattttattc
      721 agctgtgcgg ggtggcggtt aattttttgg ccggtcagga ctctggcggg aagagcgggg
      781 cg
//
LOCUS       DBBGLQ010000429          773 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.436, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000429 DBBGLQ010000000
VERSION     DBBGLQ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.436"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>773)
                     /locus_tag="ACIYSB_005830"
     CDS             complement(<1..>773)
                     /locus_tag="ACIYSB_005830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGC5432589.1"
                     /translation="RDGCFSGSAVDDNGVLSLIYTGHLCLEDRGDDSIIREVQCLATS
                     RDGIHFEKQGCVLTPPEGIMHFRDPKVWREGGYWWMVIGARDTSDTGQVLLYRGTSLR
                     DWRQERVLAQSAAGESYMWECPDFFRCGDHHWLMFSPQGMTPGGYRFRNRFQSGVLPG
                     HWQPGAAFALSGAFEELDHGHDFYAPQSLVAEDGRRIIMAWMNMWDSPMPTKREAWAG
                     CLTLPRELFERHGRLCQRPVREAESLRGTCQPLHPVRLS"
BASE COUNT          163 a          256 c          216 g          138 t
ORIGIN      
        1 actgagcctt acagggtgta acggctggca cgtcccgcga agggattcgg cttcgcgaac
       61 cggacgctga cacagacgac catggcgttc aaaaagttcc ctcggcaggg tcagacatcc
      121 ggcccaggcc tcacgctttg taggcatcgg ggagtcccac atattcatcc aggccatgat
      181 gatgcgccgt ccgtcctccg cgaccaggga ctgcggggca taaaaatcat gaccgtgatc
      241 cagttcttca aatgccccgc tcagggcaaa ggcggccccc ggctgccagt gacccggtaa
      301 aactccgctc tgaaagcggt tgcggaaccg gtatccgccg ggcgtcatcc cctgcgggga
      361 gaacatcagc cagtgatggt cgccacagcg gaaaaagtcc ggacattccc acatatagct
      421 ttctccggct gccgactgcg ccagaacccg ttcctggcgc cagtcccgca gggatgtgcc
      481 gcgatacagc agcacctgac cggtgtcaga ggtatcccgc gcgccgataa ccatccacca
      541 gtatccccct tcccgccaga ccttcgggtc acggaaatgc atgatgcctt ccggtggcgt
      601 cagcacgcag ccctgtttct caaagtgaat gccgtcacgg ctggttgcca gacactggac
      661 ttcgcggatg atgctgtcat ccccccgatc ctccagacag agatgaccgg tgtagataag
      721 cgacagcacg ccgttgtcgt ccactgcgct gccggaaaaa cagccatccc ggt
//
LOCUS       DBBGLQ010000430          771 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.437, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000430 DBBGLQ010000000
VERSION     DBBGLQ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.437"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>771
                     /locus_tag="ACIYSB_005831"
     rRNA            <1..>771
                     /locus_tag="ACIYSB_005831"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          217 a          168 c          237 g          149 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcacgcttag gcgtgtgact
      181 gcgtaccttt tgtataatgg gtcagcgact tatattctgt agcaaggtta accgaatagg
      241 ggagccgaag ggaaaccgag tcttaactgg gcgttaagtt gcagggtata gacccgaaac
      301 ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg
      361 actaatgttg aaaaattagc ggatgacttg tggctggggg tgaaaggcca atcaaaccgg
      421 gagatagctg gttctccccg aaagctattt aggtagcgcc tcgtgaattc atctccgggg
      481 gtagagcact gtttcggcaa gggggtcatc ccgacttacc aacccgatgc aaactgcgaa
      541 taccggagaa tgttatcacg ggagacacac ggcgggtgct aacgtccgtc gtgaagaggg
      601 aaacaaccca gaccgccagc taaggtccca aagtcatggt taagtgggaa acgatgtggg
      661 aaggcccaga cagccaggat gttggcttag aagcagccat catttaaaga aagcgtaata
      721 gctcactggt cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat g
//
LOCUS       DBBGLQ010000431          766 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.438, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000431 DBBGLQ010000000
VERSION     DBBGLQ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.438"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..290
                     /locus_tag="ACIYSB_005832"
     CDS             <1..290
                     /locus_tag="ACIYSB_005832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGC5432590.1"
                     /translation="QGLLCVTDLAVLERWCVAYEFWRRAVKNIARQGNTITGAMGGMV
                     KNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGKKKATNPFLKIIES"
     gene            287..>766
                     /locus_tag="ACIYSB_005833"
     CDS             287..>766
                     /locus_tag="ACIYSB_005833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGC5432591.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFVCDNEFGAEVYSGATTEKQAWEVFRPA
                     "
BASE COUNT          174 a          194 c          234 g          164 t
ORIGIN      
        1 gtcagggcct gctgtgcgtg actgacctcg cagtgcttga gcggtggtgc gtggcctacg
       61 agttctggcg acgtgccgtg aaaaatattg ccagacaggg caataccatc accggtgcaa
      121 tgggcggtat ggtcaaaaat ccggagctga ccgccaaaaa agaacaggag tccgagatga
      181 gcagtacggg ggcaatgctc ggactcgacc ccagcagccg ccagcgtctg attggcctgg
      241 cggggaagaa gaaagccact aacccgtttc tgaaaatcat cgaatcatga gccggaaatc
      301 ttaccccaac gtaaatgctg ccaatcagta tgcccgggat gtcgttcgcg gaaagattgt
      361 ggcctgccag tttgtgattc aggcctgcca gcgccatctt gatgacctga tggcggaaaa
      421 aagtaagtcg tttcgttacc gcttcgacaa ggacctggct gaacgggccg cgaaatttat
      481 tcagctgttg ccgcacacca agggtgagtg ggcattcaag aggatgccca tcacgctgga
      541 gccgtggcag ctctttgtga tctgctgtgc gtttggctgg gtcaataaag gcacccggtt
      601 gcgccgcttc cgggaggtgt ataccgaaat cccccgtaag aacggcaaat cggcaatctc
      661 tgccggtgtt gccctgtatt gttttgtctg tgataacgag tttggcgcgg aagtgtattc
      721 cggtgccacg acagagaaac aggcgtggga agtctttcgc ccggcg
//
LOCUS       DBBGLQ010000432          754 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.439, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000432 DBBGLQ010000000
VERSION     DBBGLQ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.439"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..675)
                     /locus_tag="ACIYSB_005834"
     CDS             complement(<1..675)
                     /locus_tag="ACIYSB_005834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HGC5432592.1"
                     /translation="MKKLLALTAVAALTGTFSTLTLADADKTGFYITGKAGASVITQD
                     NQRFSQDFGDDVYKYKGGDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGSANSR
                     YPLDTWESPSGDNGWKEDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSAGLGYARVHHK
                     ATYTDTSWNKSGEVSDASSLHYSGYDNNFAWSLGVGVKYEVTPDFSLDLAYRYLDAGD
                     STLTYED"
BASE COUNT          205 a          194 c          155 g          200 t
ORIGIN      
        1 gtcttcatac gtcagggttg aatcacctgc atcaagatag cgataagcaa ggtcaagact
       61 gaaatccggg gtgacctcat atttcacgcc aacaccaagg ctccatgcga aattgttatc
      121 atatcccgag taatgtaatg aagaagcatc acttacttca cctgatttat tccaggaggt
      181 atcggtgtat gttgccttat gatgtacccg cgcataaccc agtccggctg atacccacgg
      241 agtgaaagca ctgctgtttc tgaaatcata atacgtgttc accatcaggg tattcacgct
      301 gagcctgttt tgtgtgtctt ccttccaacc attatctcca gacggggact cccatgtatc
      361 cagtggatag cgggaattcg cagagccgcg accatagaat tcaacttccg tgcgtacagg
      421 gacattgtag tgctgataaa agtcgtatcc caccgcaagg ccagcaccaa aaaccgtatc
      481 gctcttgtca ccgcctttat acttataaac atcatcccca aagtcctgac taaagcgctg
      541 attatcctga gtgataacag atgcaccagc cttacccgtg atatagaaac cggttttatc
      601 cgcatctgcc agcgtcagcg ttgaaaacgt accggtaagc gcagctaccg cagtgagtgc
      661 cagtaacttt ttcacaatcc acctctcctg atgtttcctt cagacatatg aatttgtcca
      721 attttaagac ggatttctca ttggtaaaat gaat
//
LOCUS       DBBGLQ010000433          746 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.440, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000433 DBBGLQ010000000
VERSION     DBBGLQ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.440"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            2..190
                     /gene="ynfQ"
                     /locus_tag="ACIYSB_005835"
     CDS             2..190
                     /gene="ynfQ"
                     /locus_tag="ACIYSB_005835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGC5432593.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            193..258
                     /gene="ynfR"
                     /locus_tag="ACIYSB_005836"
     CDS             193..258
                     /gene="ynfR"
                     /locus_tag="ACIYSB_005836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HGC5432594.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            337..492
                     /gene="ynfN"
                     /locus_tag="ACIYSB_005837"
     CDS             337..492
                     /gene="ynfN"
                     /locus_tag="ACIYSB_005837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HGC5432595.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            664..>746
                     /locus_tag="ACIYSB_005838"
     CDS             664..>746
                     /locus_tag="ACIYSB_005838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGC5432596.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKK"
BASE COUNT          246 a          125 c          137 g          238 t
ORIGIN      
        1 tattacaaac catattcact ttagatgccc gtgttgtcat ggttcccagt atagaacatc
       61 atcttttgat gtttctgaca tgaatccttt cggggcaaaa tgtatctttt gtaaatcaat
      121 gatgattaca tttgataata tttcacaata cttaaatgcc agccgtctgt cgttggattt
      181 aaaaaagtga aaatgaaggc tccttcggga gcttttttgc ttggtgtcta ttcgatggat
      241 actcacatac tacggtaaca tcatgaaaaa aatcatagtt tttttaactc tgaaccagca
      301 gtggtagtgc cagcgatgac tggagttaac accatcatgc gtgaatatcc aaatggcgaa
      361 aaaacacacc ttactgtaat ggccgcaggg tttccatctc tgaccggaga tcataaagtc
      421 atttatgtag ccgcggatcg acatgttact tcagaagaaa ttctggaagc agcaataagg
      481 ctcttgagtt gatttgatgc tattgcattg ataattcagg aaaattctct ttgtctgttt
      541 gtgtaaaatt tagactatcg tatgttgatt attgcgatgt ttcatcttat cttttacacg
      601 tttgcaccat ataatcgact tactgtgtaa ctggaaagtc ataacagact aaaagaggaa
      661 atgatgaata ttgaaaactt aaaaacaaaa gcagaagcag atatttctga atatataaca
      721 aaaaaaatta ttgaacttaa gaaaaa
//
LOCUS       DBBGLQ010000434          743 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.441, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000434 DBBGLQ010000000
VERSION     DBBGLQ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.441"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(191..394)
                     /locus_tag="ACIYSB_005839"
     CDS             complement(191..394)
                     /locus_tag="ACIYSB_005839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2913 family protein"
                     /protein_id="HGC5432597.1"
                     /translation="MSDNIASGQLVSGKAAHLTWCGLVALHLARRDGRIASKIQENLF
                     LNRWRSTALKQHRFPRTYQSPAE"
     gene            complement(400..>743)
                     /locus_tag="ACIYSB_005840"
     CDS             complement(400..>743)
                     /locus_tag="ACIYSB_005840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGC5432598.1"
                     /translation="DTLVAPVSGTIGKILETNHAFSIRTDSGTELFVHFGINTVDLRG
                     EGFRRIAQEGQLISTGDPVIGFDLALLQQKAQSTLTPVIVADSDRVQEITLFSGPVIA
                     GETPVFDISSQ"
BASE COUNT          149 a          226 c          203 g          165 t
ORIGIN      
        1 atccccgttc ggaaccacga cattacgccc ctcccatttc cggtcgctca gtatccgcca
       61 gcaccagccc tgctccctga tggtttccag ggcgaatgtc aggcggaaca ggtcagtctg
      121 taccgacaca tcaccagagc aggcctgcca cagatactcc agcctgctgc ggatatcttt
      181 cgccccgctt tcactctgca gggctctgat atgtgcgggg aaaacggtgc tgtttcagtg
      241 ctgtgctccg ccagcggttt agaaacaggt tttcctggat ttttgacgca atccggccat
      301 cccggcgggc cagatgcagg gctaccagcc cgcaccaggt cagatgtgcg gctttccctg
      361 ataccagctg cccgctggcg atattgtcac tcatggcgct tactggctgc tgatatcaaa
      421 aaccggtgtt tctccggcga tgaccgggcc ggagaacagg gttatctcct ggacacggtc
      481 agagtccgcc acaatcaccg gtgtcagcgt ggactgtgct ttctgctgaa gtaacgcaag
      541 gtcaaacccg atgacggggt caccggtgct gatcagctgg ccttcctggg caatacgccg
      601 gaaaccctct cccctgaggt caactgtgtt aataccgaag tgcacgaaca gttcagtgcc
      661 actgtcggtg cggatggaaa acgcgtggtt tgtctccagt attttaccga tagtgccgct
      721 gacaggagcc accaaggtat cgc
//
LOCUS       DBBGLQ010000435          741 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.442, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000435 DBBGLQ010000000
VERSION     DBBGLQ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.442"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..317)
                     /locus_tag="ACIYSB_005841"
     CDS             complement(<1..317)
                     /locus_tag="ACIYSB_005841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGC5432599.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEDKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNS"
     gene            complement(407..640)
                     /locus_tag="ACIYSB_005842"
     CDS             complement(407..640)
                     /locus_tag="ACIYSB_005842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGC5432600.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
BASE COUNT          167 a          191 c          205 g          178 t
ORIGIN      
        1 tgggagttca gcaggatgat ttcagggaca tgttgtccgt ttatctcccc gtcacggcgc
       61 agacgcagca tgtgtttggt atacccccgg cggcccgggt cacgtacgcg ggtctggcag
      121 gcaaagaagg gctgaaagcc ttcccgctgc aggctttcca gcacggtgat ggtggggatc
      181 caggtatagt tctcgcttct ggatgtgtgc ttatcttccc cgaatacgct ggggacgacc
      241 tgcatcagtt cttcgcgagt taaggggcgg tcacggcgta tctggttcgc ataaccaaaa
      301 cgacttgcta atcgcatgat ttgctcctta tgaatgagtt agcgatataa atgaaaaagc
      361 cgcgcctccc ggagaagacg cggcctgatg gatgaaatga agacgtttat tgcctgagaa
      421 ttccctcact ggcgagatac ggatttagct gttttccggc atcaggttca aaattccagc
      481 accgccagcg tagctgccct tcggcattgc ggataaccag ccggaaatgc gttccctggt
      541 catcttcaat aagcacattg cggtaagctg ttgtgacagc catcgcctgt tcgcgtgaga
      601 aagggccttc gggcaataaa acagggtgac agtccgacat accggctatt ttccgataaa
      661 ttcaacgctg gcaccggtgg cattaccgtc agcaccaacc gtgacgatca ctttgtcgta
      721 cgcacggtct tcaggattga a
//
LOCUS       DBBGLQ010000436          730 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.443, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000436 DBBGLQ010000000
VERSION     DBBGLQ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.443"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>730)
                     /locus_tag="ACIYSB_005843"
     CDS             complement(<1..>730)
                     /locus_tag="ACIYSB_005843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004149557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGC5432601.1"
                     /translation="GLKRFLHVGTAMSCTPHTGSLVKEESASSETGEHLVEYTHSKAT
                     IEYLMRKQCPDLPLLVARPSIIVGHSRLGCLPSTSIFWVFIMGLMLQKFMCSLDDKID
                     VIPVDYCADALLMLLESSLINGEIVHISAGKESSVTFSAIDEAVARALNCVPVGDRYT
                     KVSYDILAMSRHDFKNIFGPCNERLMLKAIRLYGAFSMLNVCFSNDKLLSIGMPKPPK
                     FTDYIKYCIETTKHLSIQQQMEVD"
BASE COUNT          236 a          155 c          129 g          210 t
ORIGIN      
        1 aaatcaactt ccatttgttg ttgaattgaa aggtgttttg tcgtttctat acagtattta
       61 atataatcag taaactttgg cggtttaggc attccgatac tcagtagctt gtcgttactg
      121 aaacaaacat tgagcatact gaacgctcca tataaacgaa tggcttttaa cataaggcgt
      181 tcgttacagg gaccaaaaat atttttaaag tcatgacggc tcattgccag tatgtcataa
      241 ctgactttag tatatctgtc tccaacagga acacagttca aagcacgggc tacagcttcg
      301 tcaatagcag agaacgtcac actactttct ttacctgctg atatatgaac aatctcacca
      361 ttaattaacg agctttcaag caacattagc aatgcatcag cacaataatc tacagggata
      421 acatctattt tatcatccag agagcacata aatttttgca acattaaccc cattatgaat
      481 acccagaaaa tactggttga aggtaagcac cctaaacgac tgtggccaac aataattgat
      541 ggtcgggcaa ccaacaaagg taaatcagga cactgcttac gcatcagata ttctattgtt
      601 gcttttgaat gcgtatactc cactaaatgt tcacctgttt ctgatgaagc agactcttcc
      661 ttaactagcg accccgtatg aggtgtacaa gacatagcag taccaacatg aaggaagcgt
      721 ttcagtcctg
//
LOCUS       DBBGLQ010000437          713 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.444, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000437 DBBGLQ010000000
VERSION     DBBGLQ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.444"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(129..>713)
                     /locus_tag="ACIYSB_005844"
     CDS             complement(129..>713)
                     /locus_tag="ACIYSB_005844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGC5432602.1"
                     /translation="EAWINSELATEFAEQEEIAFTSGDGTKKPKGFLAYESTDETDKV
                     RAFGKLQHIVSGEATTVTADAIIKLIYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGN
                     YLWRPGLELGQPSSLAGYGIAENEQMPDIAADAKAIAFGNFKRGYTIVDRIGTRILRD
                     PYTNKPFVGFYTTKRTGGMLVDSQAIKLLKIAAA"
BASE COUNT          147 a          203 c          185 g          178 t
ORIGIN      
        1 cgtcgtcata ctcaccggcc cgaatgacct caacacgcat accgtccggt gaccatttca
       61 gatcttgttt caggatcatg attcttcacc cgtcagaaca gggggcgcgg ttccgcgccc
      121 ctgaatgatt acgccgctgc aatcttcagc agtttgatgg cctgcgaatc gaccagcatc
      181 ccgccggtgc gcttggtggt ataaaaaccg acaaacggtt tattggtgta cggatcacgc
      241 agaatgcggg tgccgatacg gtcaacgatg gtgtaacccc gtttgaagtt accaaatgca
      301 atggctttcg catcagcggc gatatccggc atctgttcgt tttcagcgat accgtaaccc
      361 gccagagagg acggctgccc cagttccagc cccggacgcc acagatagtt accctcgctg
      421 tctttcagca gacggatggc aaacaggctg ttgttgttca tcatgaactt cgcgccagtg
      481 cggtgtgcct tacgtagcgt gtaaatcagt ttgataatgg cgtctgcggt caccgtcgtc
      541 gcttcaccgg atacaatatg ctgaagtttg ccgaacgccc ggaccttgtc ggtttcatca
      601 gtggattcat acgccaggaa ccctttcggc ttcttggtgc catcgcctga ggtaaaggca
      661 atttcttcct gttcggcaaa ttcggttgcc agctcgctgt tgatccaggc ctc
//
LOCUS       DBBGLQ010000438          712 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.445, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000438 DBBGLQ010000000
VERSION     DBBGLQ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.445"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>712
                     /locus_tag="ACIYSB_005845"
     CDS             <1..>712
                     /locus_tag="ACIYSB_005845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGC5432603.1"
                     /translation="EHGYIVKTAQSGGASFHILSLYDHLLVCNKDVPLFNRFASREVH
                     AAESLLAPGAKFSDRLGHSGDKFPLAKAQRDALSHFLDAKHGDILAVNGPPGTGKTTL
                     VLSIIATQWARAALEKSEPPVIIATSTNNQAVTNIIEAFGKDFSQGSGAMAGRWLPEL
                     KSFGAYFPSGSRKAEAAKKYQTEDFFNQVESKEYVEDALLFYLEKAKAAFPEKECSSP
                     EKVIELLHGQLAAKSEQLI"
BASE COUNT          185 a          170 c          177 g          180 t
ORIGIN      
        1 gagcacggtt atattgttaa aacggcgcaa tctggcggtg ccagtttcca tatcctttct
       61 ctttatgatc acctgcttgt ttgcaataag gatgtgccgc tcttcaatcg attcgcctcg
      121 cgagaggttc atgctgcaga gtctttgctg gcccctggag caaaattcag cgacaggctt
      181 ggacactccg gagataagtt tccgctggca aaggctcagc gcgatgcctt aagccatttt
      241 ctggatgcta aacatggcga tatccttgct gttaatggcc ctcctggaac cggaaaaacc
      301 acgctggtgc tttctatcat cgccacgcag tgggccagag cggctctcga aaaatctgag
      361 cctccggtta ttatcgcgac ttcaacgaat aaccaggctg taacgaacat tattgaggca
      421 ttcgggaaag acttttcgca aggttcaggt gcgatggccg ggcgatggtt gccagagctg
      481 aaaagcttcg gcgcttattt tccctcaggc agccgtaaag ctgaagcagc caaaaaatat
      541 caaactgaag atttcttcaa ccaggttgag tcaaaagagt atgtagaaga tgcactgctg
      601 ttttatctgg aaaaggctaa ggcagccttt cctgaaaaag agtgttcatc ccctgaaaag
      661 gtcattgaac tcctgcatgg tcagttggca gcaaaatccg agcaactgat aa
//
LOCUS       DBBGLQ010000439          710 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.446, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000439 DBBGLQ010000000
VERSION     DBBGLQ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.446"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..621
                     /locus_tag="ACIYSB_005846"
     CDS             <1..621
                     /locus_tag="ACIYSB_005846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5432604.1"
                     /translation="TAGLYATWYANDETHNGSYLDSWLQYSWFDNHINGERLPAESWK
                     SKGFTVSLEAGYSWKAGEFTDNYKGSHEWYVQPQLQVVRMNVKSDRHRESNGTSIENT
                     GNGNILTRLGARTWFVSKNGKNSRYAVPLRPFVEVNWLHNSRVFGTSMNGVSIYQDGA
                     HNIGEINGGVVGVITPEVVLRADAGIQLGEHGYRNASAMFGVEYRF"
BASE COUNT          210 a          118 c          191 g          191 t
ORIGIN      
        1 actgcgggtc tttatgcaac atggtatgcc aatgatgaaa cacacaatgg ttcttatctt
       61 gatagctggc tgcaatacag ctggtttgat aatcatataa atggagaacg gctgcctgct
      121 gagtcatgga agtcaaaagg gtttacggta tctctggaag caggatattc atggaaggct
      181 ggagagttta ccgacaatta caagggaagt catgaatggt atgttcagcc gcagcttcag
      241 gttgtccgga tgaatgtaaa atcagacaga catcgtgaaa gtaacggaac cagtattgaa
      301 aataccggta acggaaatat tctcactcgc ctgggagcga gaacatggtt tgtcagtaaa
      361 aacggtaaga attcacggta tgcagttcct ctcaggccat ttgtagaagt aaactggctg
      421 cacaatagtc gtgttttcgg caccagtatg aatggtgtaa gtatttacca ggatggggcg
      481 cataatatcg gagaaataaa tggtggtgtt gtgggagtga taactccaga agtggtattg
      541 cgggctgatg caggcattca acttggagag catggatacc gcaatgcatc tgctatgttc
      601 ggcgtggaat atcgtttctg atgactcttt acattatgaa cattcttcgc aatatattgt
      661 ttataaggca tggttagctg ggggaagcgg aaaatatgaa gacagtaatg
//
LOCUS       DBBGLQ010000440          704 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.447, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000440 DBBGLQ010000000
VERSION     DBBGLQ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.447"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>704)
                     /locus_tag="ACIYSB_005847"
     CDS             complement(<1..>704)
                     /locus_tag="ACIYSB_005847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGC5432605.1"
                     /translation="VMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSA
                     ATTRDQARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGL
                     NIHCAIIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKV
                     LRGYNSDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKK
                     AKEQVSARVNFFTKHM"
BASE COUNT          157 a          203 c          164 g          180 t
ORIGIN      
        1 catgtgtttg gtaaaaaaat tcacccgcgc agagacctgt tctttcgctt ttttcgccag
       61 gcgacgcaga tcatcccagc gtttacagat gcccaggccg ggattcgctt tctgccagac
      121 cgtttcatca aacggatcat ctccctcatc gagggtgtaa ataatcgcaa agtaggagtc
      181 gtcttttacc gcgccctcca cgtcgctgtt atagccacgc aataccttga tggcgtaatc
      241 acgctgctcg taacaaatcc cttccttgtt aaaccctgcc gtggtgatac caaataaaag
      301 ggactgcaga cgggcaccgg ttgccgtttc cagaacgtcc cacacgtcac gggttttatg
      361 tgcatgcagc tcatcaataa tggcgcagtg gatgttcaga ccgtccagat tgtttgcatc
      421 cgaggaaagc ggctcaaatt ttgatgcgct ctgctcctgg taaatcgcca gcttgttgaa
      481 atcaaacaac cgcccgagtg tcgaccgggc ttttctgacc atatttttgg cgtcttcaaa
      541 cacaattctg gcctgatcac gcgtggttgc ggctgaataa acctcagcac cgccttcacc
      601 atctgccccc gtcatataca ggccgatacc cgatgacagg gttgattttg cgtttttacg
      661 ggcgacttcg ttgtacgccg tccggaaccg gcgcaccatc accg
//
LOCUS       DBBGLQ010000441          700 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.448, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000441 DBBGLQ010000000
VERSION     DBBGLQ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.448"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            3..598
                     /locus_tag="ACIYSB_005848"
                     /pseudo
     CDS             3..598
                     /locus_tag="ACIYSB_005848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178356.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          193 a          167 c          192 g          148 t
ORIGIN      
        1 aaatgcagca tctgatggtt ggctatccta aacggccgat tatgcgttga ggctttcagt
       61 gatggctgat atagcaacaa tgagaatgaa ggctctgcac ttctgggata aacacggtat
      121 ttctgcggct tctgaagcct ttggcgtgtc ctgccgcacg ctttactggt ggcgtcagtt
      181 actgaacaag ggaggacctg aggggctaat cccgcacagt aaggcacctc tggtgcgacg
      241 aaaaaagcac tggcatcccg atgtgctgaa agagattcga cgcctcagga cagagctgcc
      301 aaacctcggt aaagagcaga tttttgttcg cctgaagccc tggtgcgcac agcgccatct
      361 gacctgcccg agtgtttcca ccatcggcag aatgatcgcc accgcacacg ataaaatgcg
      421 gatggtacca gtacgtctga gctcgcgggg taaggctctg cttgtcaaaa agcgaaccgc
      481 caaaccccac cggccaaagc actaccgtct ggtaaagacc ggtgagctca ttgggatgga
      541 cgccattgag ctcagaatgg gtgaaatgcg tcgctatatc attaccatga tcgacgaacg
      601 tgaatttggc aaaaaattac aggcaggcag aaaaagtgtg tcaggagggc ataaatacag
      661 atatgagtat aacggtcaaa caatgtactg gagtggacaa
//
LOCUS       DBBGLQ010000442          697 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.449, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000442 DBBGLQ010000000
VERSION     DBBGLQ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.449"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(66..221)
                     /gene="yrhD"
                     /locus_tag="ACIYSB_005849"
     CDS             complement(66..221)
                     /gene="yrhD"
                     /locus_tag="ACIYSB_005849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HGC5432606.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            complement(316..>697)
                     /gene="yrhA"
                     /locus_tag="ACIYSB_005850"
     CDS             complement(316..>697)
                     /gene="yrhA"
                     /locus_tag="ACIYSB_005850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HGC5432607.1"
                     /translation="NSNSTNCLEKLCNEVSILFKNQPDYLTFLRAMDGFEVNGLRLFS
                     LSIPEPSVKNLFAVNEFYRNNDDFINPDLQERLVIGDYSISIFTYDIKSNFFEIRDNI
                     GTENIFSSFSDFSSFLNEIMDSCS"
BASE COUNT          238 a          110 c          102 g          247 t
ORIGIN      
        1 acgaaataag tatctgctaa tattttatga tttctttttc agaacgtcga acgggatttt
       61 cttatttaag atatcagaat cttatgattc gggtaaaatt ctaaataaat caacatgtca
      121 ttaaaatcat catagccatt agctatttcg gctaaaatag agactacatg tcttcggtcc
      181 atctcactta aggagtgtag ttccgttgta agtttttcca tagcttgcac tgctaaattt
      241 cgaacaagga attttctgct ggtaatctct aaaaagatga catggtttac aatgattttt
      301 gtttcctttt gattattatg aacaactgtc catgatttcg tttaagaatg aagagaaatc
      361 actaaacgaa ctgaatatat tttctgtgcc aatattatct ctaatttcaa aaaagttact
      421 tttaatgtca taagtaaata ttgaaatgct ataatccccg atcactaacc gttcttgtag
      481 atcagggttt atgaaatcat cattatttct ataaaattca tttacggcaa aaaggttttt
      541 aactgaaggt tctggaatcg agaggctaaa taatcgtaat ccattaactt cgaatccatc
      601 cattgctctt aaaaaagtaa gataatcagg ttgattctta aaaagaatac taacttcatt
      661 acataacttc tccaggcaat tagttgaatt agaatta
//
LOCUS       DBBGLQ010000443          692 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.450, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000443 DBBGLQ010000000
VERSION     DBBGLQ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.450"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            247..582
                     /locus_tag="ACIYSB_005851"
     CDS             247..582
                     /locus_tag="ACIYSB_005851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGC5432608.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          213 a          155 c          154 g          170 t
ORIGIN      
        1 cgtcagaaag ataaaaatat catgtgaatt aaaaaaagaa caagtagagc attaacatta
       61 tcttaaataa taaatagagg caaaaagatt attttctttt tgcgtttcct ttcaaatgaa
      121 aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact cagggatttt gtgtataatt
      181 gcggcctttt tcggcaatct gccgtttttt ggcgcttttg ccctgctgac ttttgaggaa
      241 atccacatgt cattaccaca ctgcccaaaa tgcaactccg aatacaccta cgaagataac
      301 ggcatgtaca tctgcccgga atgtgcctac gaatggaacg acgcagaacc tgcacaggaa
      361 agcgacgagc tgatcgttaa agatgctaac ggcaatctgc tggctgacgg cgacagcgtt
      421 accatcatta aagatctgaa ggtgaaaggt agctcttcga tgctgaaaat tggcaccaaa
      481 gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg attgcaaaat cgacggtttt
      541 ggtccgatga aactgaaatc tgagtttgtg aaaaagaact gattgtattg tgatcggtaa
      601 gccggataag gcgctcgcgc cgcatccggc aacggtgcca gatgcctgat gcgacgcttg
      661 cgcgtcttat caggcctaca aattcccgca cc
//
LOCUS       DBBGLQ010000444          690 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.451, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000444 DBBGLQ010000000
VERSION     DBBGLQ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.451"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..476)
                     /locus_tag="ACIYSB_005852"
     CDS             complement(<1..476)
                     /locus_tag="ACIYSB_005852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HGC5432609.1"
                     /translation="MRFLEKLNNTDIELTALTVWIARKTFALNRVEFYEALGGMIRDG
                     TPSLRALEFLCEIETDFGEKKGTSGMYFLAKECIDSIKASGMLSGALLTWVPPEEATL
                     IRNGEERGNIADALFQVARTVKARREMTSSLIAVCLYPLLLLSLCVVNMYNAHTRI"
     gene            complement(478..>690)
                     /locus_tag="ACIYSB_005853"
     CDS             complement(478..>690)
                     /locus_tag="ACIYSB_005853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein E"
                     /protein_id="HGC5432610.1"
                     /translation="VIAEVISPDARFFQLYREKGRIEARTYWHRELGGMTRNQHLLGK
                     INSGQVDPLAAHYISPIDEDSYTLLH"
BASE COUNT          147 a          183 c          188 g          172 t
ORIGIN      
        1 atgatacggg tatgggcgtt gtacatattc accacacaga gcgaaagcag cagaaggggg
       61 tagaggcaga cggcaatgag gctggatgtc atttcccgcc gggctttaac ggttctggcc
      121 acctggaaaa gggcgtcggc aatatttccg cgctcttccc cgtttctgat taacgtggct
      181 tcctccggtg gcacccaggt taacagggca ccggataaca tgccgctggc tttgatggaa
      241 tcgatgcatt cttttgccag aaaatacatg ccggatgtgc ctttcttttc accaaaatcg
      301 gtttcgatct cgcagagaaa ttcgagtgca cgcagggacg gcgttccgtc acgtatcatg
      361 ccgcccaggg cttcgtagaa ctccacccgg ttcagggcaa aggtttttct ggctatccag
      421 acagtcagtg ctgtcagctc aatgtcggtg ttgttgagtt tttccagaaa tctcatgtca
      481 gtgcagcagc gtatagctgt cctcgtcgat gggagagatg taatgggccg ccagcggatc
      541 aacctgcccg ctgttgattt ttcccagcag atgctgattt cgtgtcatgc cgcccagctc
      601 ccggtgccag tatgttctgg cctcaatacg ccccttttcc cggtacaact ggaaaaaacg
      661 cgcatccggg gagatgactt ctgcgatcac
//
LOCUS       DBBGLQ010000445          688 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.452, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000445 DBBGLQ010000000
VERSION     DBBGLQ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.452"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..166)
                     /locus_tag="ACIYSB_005854"
     CDS             complement(<1..166)
                     /locus_tag="ACIYSB_005854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGC5432611.1"
                     /translation="MYNAILNSKFIATKAGEITVYNYDSETREYISASTEYLAVGVGI
                     PAYSCLDAPGT"
     gene            complement(166..>688)
                     /locus_tag="ACIYSB_005855"
     CDS             complement(166..>688)
                     /locus_tag="ACIYSB_005855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432612.1"
                     /translation="SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNK
                     LPDLRGEFIRGWDDGRGVDAGRALLSLQDDSFEAHRHESFFYAGISRNEIPLKNLPSS
                     DEMLTLSSTTNALSPDGIDATNSLIGNDDYNCLIEGNKNNKRTATGLSTSIVGAAETR
                     PRNISFNYIVRAA"
BASE COUNT          195 a          167 c          146 g          180 t
ORIGIN      
        1 gtgtgccagg agcatctaaa caggaatatg cagggatacc gacacccaca gcaagatatt
       61 cagttgatgc agaaatatac tcccgtgtct cactgtcata gttataaacg gtaatctctc
      121 ctgcctttgt ggcaataaat ttactattca agatggcgtt atacatcatg cagccctcac
      181 aatgtaatta aatgaaatat tacgtgggcg tgtctctgct gcaccgacaa tactggtact
      241 caaccccgtc gctgttcgtt tgttattttt atttccttca atcagacagt tgtaatcatc
      301 attaccaatt aacgaattag tggcatcaat accgtccggg gacaaggcat tagttgtaga
      361 acttaaagtc agcatctcgt ctgaacttgg aagatttttt aatggtattt cattgcgaga
      421 aatacccgcg taaaaaaagg actcatgcct gtgcgcttca aaagagtcat cctgaagact
      481 tagcaaggca cgtcccgcat ccactccacg cccgtcatcc cagccacgaa taaactcacc
      541 acgtaaatca ggcaatttat ttgtcggata agcctttgcc agttccgggt attcttctgc
      601 agaaaaagcg gcaccgttgc atttcagcca gcctgttggc ggagtggcgg aaggccacgg
      661 aacaggcacg ccaacgggta atgccgaa
//
LOCUS       DBBGLQ010000446          687 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.453, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000446 DBBGLQ010000000
VERSION     DBBGLQ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.453"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            65..364
                     /locus_tag="ACIYSB_005856"
                     /pseudo
     CDS             65..364
                     /locus_tag="ACIYSB_005856"
                     /inference="COORDINATES: protein motif:HMM:NF025361.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebF family protein"
BASE COUNT          190 a          146 c          156 g          195 t
ORIGIN      
        1 ggttctgtaa ataaacaaaa cactctcttc aattctgctc accttcggta tttgtgtatc
       61 atcactggct actgataaaa acagaaccgt cagttttcca cgatgtgaag ggcaggaatc
      121 atcggctgtt gcagccagcg tcaaacggga ctatctccag aaacaggtgc ttcactggaa
      181 agatgactag gtgatcttca gtcagcagca tcctgttgtc tgggtgaatc ttcgtgagct
      241 ggtaagtaaa ggtgataatt tagtcacagt gcatctcacc gcccggggga agaaagccga
      301 tattcagtat cgggtgcgca ttgactgtaa gaacgaaaac gctatctggc agcgccagag
      361 atgaacgtat ggagataatc acagagtatg aacaaacgga ctctacatgt tcattatgac
      421 taaaaaacgc tgttttattt tacgttgctg tcataacgtg tttatctcaa ttttcgggaa
      481 catgttctta gcggttagac tggccccctg aatctcctgc gtttgaagga cagtaacagg
      541 ttaagtgata acagatgtat gaaaatatag agatacatcc aaggaagagc cacttttgcg
      601 ccgttcgtga cagttttgtc atgtccttgc tccggcctgg tttatgttca taagtccttc
      661 ttcaccagta tctttgtaaa ccttcac
//
LOCUS       DBBGLQ010000447          684 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.454, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000447 DBBGLQ010000000
VERSION     DBBGLQ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.454"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..251
                     /locus_tag="ACIYSB_005857"
                     /pseudo
     CDS             1..251
                     /locus_tag="ACIYSB_005857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          181 a          182 c          173 g          148 t
ORIGIN      
        1 ctacacctct ggctgtcaaa actctccatg atttggttga tacaaaagcc ccgatcaaca
       61 gcccgaatct gacaggaacg ccaacaacac caacagcgcc acaggggact aataataccc
      121 agatcgcaag cacggctttc gttatggccg cgattgccgc acttgtagat tcgtcacctg
      181 atgcacttaa cactctgaac gaactggccg ccgcgcttgg taatgatccg aacttttccg
      241 cgacagtgac tcgcctgatt ggagaaaaag ttgccaaaga tggcgacacc atgacaggta
      301 aacttaatct gccgcagacc tctgcattcg gcatcaatac caacaacaga ctggggggga
      361 actctctgac gctgggcgat cacgataccg gaattaaaca ggaaggagac ggcgtattgt
      421 ccgtgttcgc caacggccag cgtgtgttcc ttttcacgga aagaagtatt acctcattaa
      481 aagagcttct gataggggat gcacgttata gcaccggtgg tgatgtgtat ggcagtcagt
      541 ggggagacgg ttggttgagt tcctggttaa gtgctcgctt tgcagagtgt gccaacgcca
      601 gccataacca cccttggagc cagattaccg acgtaccagc agccagcctc actcagagcg
      661 gcattgtaca acttagtagt gcca
//
LOCUS       DBBGLQ010000448          682 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.455, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000448 DBBGLQ010000000
VERSION     DBBGLQ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.455"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..680
                     /locus_tag="ACIYSB_005858"
     CDS             <1..680
                     /locus_tag="ACIYSB_005858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5432613.1"
                     /translation="NSTNALSTGYHSEGRMNGYTAGLYATWYANDETHNGSYLDSWLQ
                     YSWFDNHINGERLPAESWKSKGFTVSLEAGYSWKAGEFTDNYKGSHEWYVQPQLQVVR
                     MNVKSDKYHESNGTSIENTGNGNILTRLGARTWLTSKNGKNTRYAVPFRPFVEAHWLH
                     NSRVFGTSMNGVSIYQDGARDIGEINGGVVGMITPEVAFRADAGIQLGEHGYHNTSAM
                     LSVEYRF"
BASE COUNT          212 a          122 c          180 g          168 t
ORIGIN      
        1 ataacagtac gaatgccctg agcaccggat accattcgga aggaagaatg aatggataca
       61 cagcgggtct ttatgcaaca tggtatgcca atgatgaaac acacaatggt tcttatcttg
      121 atagctggct gcaatacagc tggtttgata atcatataaa tggagaacgg ctgcctgctg
      181 agtcatggaa gtcaaaaggg tttacggtat ctctggaagc aggatattca tggaaggctg
      241 gagagtttac cgacaattac aagggaagtc atgaatggta tgttcagccg cagcttcagg
      301 ttgtccggat gaatgtaaaa tcagacaaat atcatgaaag taacggaacc agtattgaaa
      361 ataccggtaa cggaaatatt ctcacccgcc tgggagcaag aacatggctt accagcaaaa
      421 acggtaaaaa tacgcggtat gcggttccgt tcagaccatt tgtggaggca cactggttgc
      481 acaatagtcg tgttttcggc accagtatga atggtgtaag tatataccag gatggtgcgc
      541 gtgatatcgg agaaataaat ggtggtgttg tgggaatgat aacaccagaa gtagcattcc
      601 gggctgatgc aggcattcaa cttggagaac atggatacca taatacatct gccatgttga
      661 gtgtggaata tcgtttctga tg
//
LOCUS       DBBGLQ010000449          674 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.456, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000449 DBBGLQ010000000
VERSION     DBBGLQ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.456"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(257..550)
                     /locus_tag="ACIYSB_005859"
     CDS             complement(257..550)
                     /locus_tag="ACIYSB_005859"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGC5432614.1"
                     /translation="MNTRKTEENRKQKLLKQIEQQRQALGLLKADWLRITSPVDRGWQ
                     SLYQWRNVIVPAVGVAALYTLKSRPRRLIVWPRRVLAVWGGLRFIRRPFTSLR"
BASE COUNT          190 a          164 c          140 g          180 t
ORIGIN      
        1 aaatggcatg tcaagattta atgatagtga ttatcattaa catgatgaag ggggagttat
       61 accgttcgga aagcctgcta acgctgtggc agcatcacag tctgagtatt ctcaggagaa
      121 atacaaaaac cggacatacc ccataacagc aacaatgttt ttgcactcag ccactgtcag
      181 aaaagaactg aacgtttttc cgtcaacgga gtaacactgc cagaacaacg cctctctgaa
      241 tacgatataa attactctat cggagcgacg tgaagggccg cctgataaat cttagccctc
      301 cccagactgc cagtactctg cgtggccaga caatcagacg acgaggtctg gatttcagcg
      361 tatacaatgc agccactcca actgcgggaa cgataacatt acgccactga tataatgact
      421 gccagccgcg gtcaacaggc gacgttatcc tgagccagtc tgcctttaac aggcccagtg
      481 cctgtcgttg ttgctcaatc tgttttaaca gtttctgttt tcggttctcc tctgttttcc
      541 gggtattcat tcatcatccc ttttcagaca gttcaggtct tcactgagtt gtttacgggt
      601 ctctgatagt atcctgcttc cggcagtctt ttgtttaatg aaaatcacaa aaaataatgc
      661 aataaccaga aaaa
//
LOCUS       DBBGLQ010000450          654 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.457, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000450 DBBGLQ010000000
VERSION     DBBGLQ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.457"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..592)
                     /locus_tag="ACIYSB_005860"
     CDS             complement(<1..592)
                     /locus_tag="ACIYSB_005860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein"
                     /protein_id="HGC5432615.1"
                     /translation="MLKRRDAFLKKSALAVSVALLLSAQAQAQDILIGPIQPGDHSSF
                     LFGNSVAGRSSGDIRNVWLIGDDSFLLDSNRTVLLGNNSFVLGSPGSVSLGHDALIAD
                     SEWGTVAGKEASLISSRQSSAIGAFSSVQDSTSSVALGHGSQVSGENNVVSVGAGPEG
                     YGESVKGAPETRRIINVSDGINNTDAATVGQLNERFD"
BASE COUNT          191 a          187 c          120 g          156 t
ORIGIN      
        1 catcaaatcg ttcattcagt tgccctaccg tcgctgcatc tgtgttattg ataccatcag
       61 acacatttat aatccggcgg gtttccggtg ctcctttgac actctctcca tatccctcag
      121 gcccggcacc aacagatacc acgttatttt ctcccgacac ctgtgagcca tggcccagag
      181 ctacagaact ggtagaatcc tgcacagatg aaaacgcacc aattgccgag ctctgacgag
      241 aagaaataag tgacgcttct tttcctgcaa cagtccccca ttcagagtca gcaataagtg
      301 catcatgccc cagactcaca cttcccggag accccagaac aaaagaatta ttccccaaaa
      361 gcacagtcct gtttgagtct aataaaaaag aatcatcacc aatcaaccat acatttctta
      421 tgtcaccaga cgaacggcca gctactgaat tcccaaaaag aaagctacta tgatcgccag
      481 gttgaatcgg tccgattaaa atatcctgag cctgagcctg agccgagagc agcagggcca
      541 cactcactgc cagcgcgctt ttctttaaaa aagcatcacg tcttttaagc attctgttat
      601 tctcctgtat tattacttca ccagccagaa cggtagtgcg gagcaaaaat acag
//
LOCUS       DBBGLQ010000451          644 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.458, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000451 DBBGLQ010000000
VERSION     DBBGLQ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.458"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            27..372
                     /locus_tag="ACIYSB_005861"
                     /pseudo
     CDS             27..372
                     /locus_tag="ACIYSB_005861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166950.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
     gene            complement(390..>644)
                     /locus_tag="ACIYSB_005862"
     CDS             complement(390..>644)
                     /locus_tag="ACIYSB_005862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432616.1"
                     /translation="SICHPRVSATVDEECSQRVDLLQQTWRVISADGQCRVERCFQAA
                     RGDTSGQYIALKTVALSLGLPVVTGITHRPVQRCTLITAQ"
BASE COUNT          182 a          174 c          156 g          132 t
ORIGIN      
        1 acctgctgat aaaacaccgt ggcgacatga caaaacgcca gagcaaaaac cgtaagcgca
       61 ttattcgtct tgtggaactc tggcctgagt cattcaaccg ggaaaaaaac aaaaccactt
      121 aaagtgggga tacctgacga cctgatacag gatatcgcca tccgagaact ggcgtttggc
      181 gctggtacat tacatgcagc agtggcgtct tatgtgcagt ctccgcgcta ttaccgtgcg
      241 ttaatagcag gtggcgcccg ttacaacctg aacggtcagc cgtgcgggga agtgacacca
      301 caggaacaga aagaggcaga gacccgttta atgatgctga atgaccgacg aaaagacaga
      361 aaacctcgtt gactgcacaa cagcgctgtt cactgagctg taatcaatgt acagcgctgt
      421 accgggcgat gggtaatacc ggtcacaacc ggtaacccca gagacaatgc aactgttttt
      481 aacgcaatat actggccgga tgtatcaccc cgggcagcct gaaaacacct ttccacccgg
      541 cattgtccgt cagcacttat cacccgccat gtctgttgca ataaatccac ccgttgcgaa
      601 cattcttcat caaccgtggc actgacccgt ggatggcaaa tgct
//
LOCUS       DBBGLQ010000452          635 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.459, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000452 DBBGLQ010000000
VERSION     DBBGLQ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.459"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            76..>635
                     /locus_tag="ACIYSB_005863"
     CDS             76..>635
                     /locus_tag="ACIYSB_005863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5432617.1"
                     /translation="MALTDAKIRAAKHTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRNITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPAL"
BASE COUNT          179 a          160 c          140 g          156 t
ORIGIN      
        1 gtgtgggtat aattgcgggt ataccccagt tcgacagaat ttgtaccctc tttaacgcat
       61 tcagaaggct gaactatggc actgactgac gcaaaaatcc gggctgcaaa gcacactgac
      121 aaggcttata aactcactga cggggctggc atgttcctgc tggtacatcc taatggttcc
      181 cgttactggc gtctccgtta tcgcattctg ggtaaggaga aaactctggc acttggtgta
      241 tacccggagg tttctctctc cgaagctcgt acaaaacggg atgaggcccg aaaactgatt
      301 tcggagggga ttgacccttg cgaacagaaa agagctaaaa aagtagtccc tgatttacaa
      361 ctctcttttg aacatattgc acgacgctgg catgccagta ataaacaatg ggcacaatca
      421 cacagcgata aggtactcaa aagcctcgaa acacacgttt tcccctttat cggcaaccgg
      481 aacatcacaa cactcaatac cccggacctg cttatccctg ttcgtgctgc agaagctaaa
      541 caaatttatg aaatcgccag tcgtctgcag caaagaatat ctgctgtaat gcgttatgcc
      601 gtacagtctg gcatcatcag atataatcct gctct
//
LOCUS       DBBGLQ010000453          626 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.460, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000453 DBBGLQ010000000
VERSION     DBBGLQ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.460"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>626
                     /locus_tag="ACIYSB_005864"
     CDS             <1..>626
                     /locus_tag="ACIYSB_005864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432618.1"
                     /translation="SMQVRKSLHKGVLRSREWLNTQELLSRKNISVTGDSFKSYIKDI
                     VRLYCYKFLYSREYLNKNAYKKQYQRVINPREFNVPILQLEVAWINNNYRSSDSSSDI
                     IILPRMIRRMVVFNDTKINKQLEEVLGGVARKKVAVRNKGGLYTRIKKENDFLSMDRF
                     MVEIENIIRPEIFPTSIYPPFSLSFIERELPLLNLAHSQYKRAISKKE"
BASE COUNT          242 a           97 c          116 g          171 t
ORIGIN      
        1 ttcaatgcaa gtaagaaaaa gtcttcataa gggagtactc cggtcacggg aatggctcaa
       61 tacacaggag ttactgagca gaaaaaatat atctgttaca ggtgactcat ttaaatctta
      121 cataaaggat attgtcagat tatattgcta taagtttctc tacagcagag aatatcttaa
      181 taaaaacgca tacaaaaaac aatatcagag agttattaac cccagagaat ttaatgttcc
      241 gatacttcaa cttgaagttg catggatcaa taataattac cgttcaagtg attcaagctc
      301 tgatattatc atccttccca ggatgataag aagaatggtt gtatttaatg atacgaagat
      361 aaataaacaa ttagaggaag tgttgggggg ggtagcacga aaaaaagtcg cagtaagaaa
      421 taaaggcggt ctctatacca gaataaaaaa agaaaatgat tttctttcta tggatagatt
      481 tatggtggaa atagaaaata tcataagacc tgaaatattt ccgacatcaa tttacccgcc
      541 tttttcactt tcatttatcg agagagaatt gccactactg aatctggcac actcacagta
      601 caaaagagca ataagtaaaa aagagg
//
LOCUS       DBBGLQ010000454          617 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.461, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000454 DBBGLQ010000000
VERSION     DBBGLQ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.461"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..523
                     /locus_tag="ACIYSB_005865"
     CDS             <1..523
                     /locus_tag="ACIYSB_005865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350955.1"
                     /note="induced within macrophages; necessary for the
                     resistance to antimicrobials; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HGC5432619.1"
                     /translation="NCIIKESFSPKFEKTRRNEIQRFIRNGGEIKCISQLSDKEISSS
                     YISLFHSRFGGTLPCYEYDNLLMFISHLRELMFGHVLFWDNKPCAIDIVLKSESSCNV
                     YYDVPNGAVLNDENCMKLSPGSVLMWLNIDKARRYCQDNNKKMIYSIGAFRPEWKYKL
                     LWSVPCKVGKCLC"
BASE COUNT          207 a           91 c          120 g          199 t
ORIGIN      
        1 aaattgtatt ataaaggaat ctttttcacc taaatttgaa aaaactcgcc gaaatgaaat
       61 ccagagattt atcaggaatg gcggagagat taagtgtata agccagttat ccgataaaga
      121 aatatccagc tcttatatat cactttttca ttcccggttt ggaggtaccc taccttgcta
      181 cgaatatgat aatctgttaa tgtttatttc tcacttacga gaacttatgt ttggccatgt
      241 gctattctgg gataataaac cgtgtgctat tgatatagta ctgaaatctg aaagctcatg
      301 taatgtttat tatgacgttc caaatggtgc agttctgaat gatgagaatt gcatgaaact
      361 aagtccggga agtgtcctta tgtggctgaa tatagataaa gcacgaaggt attgtcagga
      421 taataataaa aagatgatat attctatcgg tgcattcaga cctgaatgga aatataaact
      481 actatggtct gttccctgta aggttggtaa atgcttatgt tagaatatgt ataaaggtaa
      541 gtaactatat aagagcgtcc agtctgcaaa tactcctcta aacatggagg agtagattta
      601 taatatcata acattta
//
LOCUS       DBBGLQ010000455          616 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.462, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000455 DBBGLQ010000000
VERSION     DBBGLQ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.462"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            3..500
                     /locus_tag="ACIYSB_005866"
     CDS             3..500
                     /locus_tag="ACIYSB_005866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGC5432620.1"
                     /translation="MLAICLMFINEICQATDCFDLAGRDYKIDPDLLRAISWKESRYR
                     VNVIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKK
                     WGVTWEAVGAYNAGFRKSERQNQRRLAYASEVYRIYTGIKSSKGIRLPATKKSLPEIN
                     SVQNN"
BASE COUNT          187 a          140 c          150 g          139 t
ORIGIN      
        1 ggatgttagc catctgcctg atgtttataa atgagatctg ccaggccact gattgctttg
       61 atcttgcagg ccgggattac aaaatagatc ctgatttact gagagcaata tcatggaaag
      121 aatcccgtta ccgggttaat gtcatcggta ttaatccggt aacgggatat ggcagcggac
      181 tgatgcaggt agattcccag cattttaacg aactggcccg ctacggaatt aagccggaac
      241 atctgacaac agatccctgc atgaacattt ataccggtgc ttattatctg gcaatagcat
      301 ttaaaaaatg gggcgtcacc tgggaggccg ttggtgcata caatgcagga ttcaggaaat
      361 ccgaacgcca gaaccagaga cgtcttgcct acgcatcaga ggtttaccgg atttataccg
      421 ggataaaaag cagtaaaggt atccggcttc cggccacgaa gaaatcactt ccggaaatta
      481 acagcgtgca gaacaattaa tcttacagac attgtcagat accggttatg ccgcaaaagc
      541 ggcatggaag gccacacaca aaaatatcat atgagtgaca atactgactg gccgaagccc
      601 cgcgaagcgg gaaccc
//
LOCUS       DBBGLQ010000456          613 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.463, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000456 DBBGLQ010000000
VERSION     DBBGLQ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.463"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..178
                     /locus_tag="ACIYSB_005867"
     CDS             <1..178
                     /locus_tag="ACIYSB_005867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432621.1"
                     /translation="EDSSSEELLSDNTLEKDENSPDNGFEVGEYNTYEAYNSEKQYFT
                     REDYTYDYDLLNAI"
BASE COUNT          190 a          134 c          126 g          163 t
ORIGIN      
        1 agaagactct agctcagaag agttactttc agataatacc ctcgagaaag acgaaaattc
       61 tccggacaat ggctttgagg tcggggaata taacacatat gaagcatata actcagagaa
      121 gcaatatttt accagagagg actatacgta tgattacgac cttttaaatg caatatagac
      181 ttaataactt cttacaaagt ctattattaa taccgtagat atttattcat cttaccctgt
      241 gcaccaaaag caaaaactca ctgacgcggg tttgaaactg atggcaaccg caaaaatgcc
      301 tgatgcgcta cgcttatcag gcctacgcca tctctgcaat atattgaatt tgcgtgcttt
      361 tgtaggcagg ataaggcgtt cacgccgcat ccggcatgaa taaagcgcac tcaacaatct
      421 gaaacccgcc ggaacggttt attacgtaca tcaggtaaaa ctgaccgata agccgctttc
      481 ttttgggtat agtgtcgtgg acagtcattc atctttctgc ccctccaaaa gtaaaaaccc
      541 gccgaagcgg gtttttacgt aaaacaggtg aaactgaccg ataagccgct ttcttttggg
      601 tatagtgtcg tgg
//
LOCUS       DBBGLQ010000457          612 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.464, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000457 DBBGLQ010000000
VERSION     DBBGLQ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.464"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..612
                     /locus_tag="ACIYSB_005868"
                     /pseudo
     CDS             1..612
                     /locus_tag="ACIYSB_005868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          147 a          156 c          176 g          133 t
ORIGIN      
        1 aaacgcagac ggcgtaccac acaggaaaag atcgcaattg ttcagcagag ctttgaaccg
       61 gggatgacgg tctccctcgt tgcccggcaa catggtgtag cagccagcca gttatttctc
      121 tggcgtaagc aataccagga aggaagtctt actgctgtcg ccgccggaga acaggttgtt
      181 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
      241 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      301 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      361 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      421 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      481 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      541 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      601 tgttgcttga gc
//
LOCUS       DBBGLQ010000458          608 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.465, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000458 DBBGLQ010000000
VERSION     DBBGLQ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.465"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..485
                     /locus_tag="ACIYSB_005869"
                     /pseudo
     CDS             1..485
                     /locus_tag="ACIYSB_005869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc39 family transposase"
BASE COUNT          176 a          143 c          152 g          137 t
ORIGIN      
        1 aatagcgtgt acccgcagac gcatattcag ctctgcatca tccatatggt gcgcaacagc
       61 ctgaaattcg tgtcgtggaa ggattacaaa gccgtcacca gcgggctgaa agcggtatat
      121 caggccccga cagaggaagc ggcgttgatg gcgttggata agtttgcagg cgtctgggat
      181 gaaaaatacc cgcaaatcag caagagctgg cgcacacact gggaaaatct caacaccttc
      241 ttcggttacc caccggatat ccgcaaggcc atctacacga cgaatgccat cgaatcgctg
      301 aacagtgtca ttcggcaggc gataaagaag cgcaaggtgt tcccaacgga cgactcggtg
      361 cgaaaagtga tttatctggc gatacagtcg gcgtcaaaaa atggagtatg ccaatccaga
      421 actggcggct ggcaatgagc cgttttatta tcgagttcgg tgaccgcctt agcgagcacc
      481 tttaatacgg cggcagttac acagaattat ttacagggtc attctgaatt cagtgcagaa
      541 ttaaaacatg tgtgcaaaat aatttaattc agacagcttt ttatttcact atggctatcc
      601 cagaacaa
//
LOCUS       DBBGLQ010000459          597 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.466, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000459 DBBGLQ010000000
VERSION     DBBGLQ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.466"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            33..383
                     /gene="tnpB"
                     /locus_tag="ACIYSB_005870"
     CDS             33..383
                     /gene="tnpB"
                     /locus_tag="ACIYSB_005870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGC5432622.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTFRLNALTML"
     gene            414..>597
                     /locus_tag="ACIYSB_005871"
     CDS             414..>597
                     /locus_tag="ACIYSB_005871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGC5432623.1"
                     /translation="MSQKYLIRIAELESQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKL"
BASE COUNT          158 a          152 c          157 g          130 t
ORIGIN      
        1 gtgttgatcc gtgaactgac cgggagggga agatgatctc actcccatca ggtacccgta
       61 tctggctcgt tgccggcgtt accgatatgc gtaaatcctt caacggactg ggagaacagg
      121 tacaacatgt gctgaatgat aatcccttct ccggtcacct gtttatcttc cgtggccgac
      181 ggggtgacac cgttaaaatt ctttgggctg atgctgatgg tctgtgcctg ttcaccaaac
      241 gcctggagga aggccagttt atctggcctg cggtacgtga cggcaaggta tccattaccc
      301 gctcgcaact ggcaatgctc ctcgataagc tggactggcg tcagccaaaa acattccgcc
      361 ttaacgcact gacaatgttg taaaaaacgc ctggccgcat tataaaaacg gccatgagtc
      421 agaaatacct cattcgcatt gcagaactgg aaagccagct ccgtcagaaa gaccagcaac
      481 tgagtctggt tgaagagacg gaggccttcc tgcgctctgc actggcacgt gccgaagaaa
      541 agatcgaaga agatgaacgg gaaatagaac atctgcgggc tcagatagaa aaactgc
//
LOCUS       DBBGLQ010000460          595 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.467, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000460 DBBGLQ010000000
VERSION     DBBGLQ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.467"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(26..520)
                     /locus_tag="ACIYSB_005872"
     CDS             complement(26..520)
                     /locus_tag="ACIYSB_005872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGC5432624.1"
                     /translation="MSMVARPEAAPARDDIPDTDDGDATITAGAFWPEIVLRELRLAV
                     RLPGRITSTRLAHVATGAVAHVTRELEEWQKTQSAAGFSILAEVPAATINGESVNVWH
                     YRNAVYSATRALILERWRDVDTTDKGNRRADALDEQMEDLWRDVRWSVADILGRPRLF
                     AELV"
BASE COUNT          109 a          190 c          185 g          111 t
ORIGIN      
        1 tccccctgga gtgccctcac tttcatcaca ccagctccgc aaaaagacgg gggcgaccca
       61 gaatatctgc cacggaccag cgcacatcgc gccataagtc ttccatctgc tcatccagtg
      121 cgtcagcccg gcggttccct ttatcagtgg tgtccacatc ccgccagcgt tccagaatca
      181 gggcgcgggt ggcgctgtag acggcgttgc ggtaatgcca tacgttgacg ctctcaccgt
      241 tgatggtggc ggcaggcact tctgcgagga tgctgaaccc tgcggcagac tgcgtcttct
      301 gccactcttc cagctcccgc gtgacgtgag ccacggctcc cgtggcaacg tgcgccagac
      361 gtgtggaggt gatgcggccc ggcaggcgta ctgcaaggcg cagctcccgc agcacaatct
      421 ccggccagaa tgccccggcg gtgatggtgg cgtcgccgtc gtcggtgtcg gggatatcgt
      481 cccgtgcagg ggcggcttcc ggtcttgcga ccatgctcat aaatcgctcc ttcagaaaaa
      541 aaaggcggtg ggtgccgggt gtaaggggaa cggaaaaccg taaccggaac gcccg
//
LOCUS       DBBGLQ010000461          594 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.468, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000461 DBBGLQ010000000
VERSION     DBBGLQ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.468"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(124..>594)
                     /locus_tag="ACIYSB_005873"
     CDS             complement(124..>594)
                     /locus_tag="ACIYSB_005873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGC5432625.1"
                     /translation="ELSASILEDVAVVPQKAVLVQGTIRDNILYGTDDILPDDELFCI
                     LNSLGLIKEECGEHFLAFLDRDINPLGSGLSGGEIQRLCIARALARKKKIIVLDEPTA
                     SIGEELAIKIITYIRQHCPTVIITTHNPRILELADHFFDGMSKSCVIAGEGRDQ"
BASE COUNT          178 a          143 c          120 g          153 t
ORIGIN      
        1 cataaacgta cacgtcaaaa ccttccaaac atgcttcaaa aatcaacaaa ttcgctgtat
       61 cggaaatttc ggcgtttccg gcgtacaacc ccgattagat ataaatgaat taacgtgtgt
      121 ggtttattgg tctctgcctt ctccagcaat gacgcaagac tttgacatac catcaaaaaa
      181 atgatcggcg agctccagaa ttcgtggatt atgagttgta ataataacgg tagggcaatg
      241 ctgccggata taggtaatga tctttatcgc gagttcttcg ccaatagagg ccgtgggttc
      301 atcaagtacg ataatctttt tcttacgggc tagggcgcgg gctatgcata atcgctggat
      361 ctctccacca ctcaaaccac tacccagagg attaatatcc cggtctagga aagcaaggaa
      421 atgttctccg cactcctctt taattaaccc caatgaattc aggatgcaaa acagttcatc
      481 atcgggaaga atgtcatcgg ttccatacag aatattatca cgaatagttc cctgcaccag
      541 aacggctttt tgaggcacaa cagcaacgtc ctccaggata gacgcactca actc
//
LOCUS       DBBGLQ010000462          593 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.469, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000462 DBBGLQ010000000
VERSION     DBBGLQ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.469"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>593
                     /locus_tag="ACIYSB_005874"
     rRNA            <1..>593
                     /locus_tag="ACIYSB_005874"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          166 a          130 c          184 g          113 t
ORIGIN      
        1 cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg aagaccaagg
       61 gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag gccgaaaggc
      121 gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa ggggggacgg
      181 agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc tggttttcca
      241 ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg ctgaagcaac
      301 aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc gtaccccaaa
      361 ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg gtgaaggaac
      421 taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt gaagcgactt
      481 gctcgtggag ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac
      541 agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg tgc
//
LOCUS       DBBGLQ010000463          590 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.470, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000463 DBBGLQ010000000
VERSION     DBBGLQ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.470"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..123
                     /locus_tag="ACIYSB_005875"
     CDS             <1..123
                     /locus_tag="ACIYSB_005875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HGC5432626.1"
                     /translation="GTEAYTGREVQDIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            138..>590
                     /locus_tag="ACIYSB_005876"
     CDS             138..>590
                     /locus_tag="ACIYSB_005876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HGC5432627.1"
                     /translation="MSRIITITVYTLDELSCPAREKARDGYRQHHADSSWYENVYEDF
                     RAVCDIVGIALRQRVIRLSSGRFMEEPCIWFSGFSSQGDGACFDGRWHWQPATARKIR
                     EYAPQDRELHRIADALQAVQKRNFRQLQAEIRHRGRYCHPYSMDITVTR"
BASE COUNT          126 a          160 c          185 g          119 t
ORIGIN      
        1 ggcactgaag cgtataccgg tcgtgaagtg caggatattc ccggtgtgct ggccgtgttt
       61 gcagaacgcc gtaaggacag ttttggtccg tatgtccggc tgatgagcgt caccctgaac
      121 tgagtcaggg cattctgatg agcagaatta tcaccatcac agtgtatacg ctggatgaac
      181 tttcctgccc ggcacgggaa aaagcccgcg acgggtaccg tcagcatcat gctgacagca
      241 gctggtatga aaacgtgtat gaggacttca gggcggtctg tgacattgtc gggattgccc
      301 tccgccagcg agttatccgg ctcagcagcg gcaggtttat ggaagagccg tgcatctggt
      361 tcagcggctt cagcagccag ggggacggag cctgttttga cgggcgctgg cactggcagc
      421 cggcaacagc caggaaaatc cgggaatatg ccccgcagga ccgtgagctt catcgtatcg
      481 cggatgccct gcaggcggtg cagaagcgta acttccggca gcttcaggct gaaatccgcc
      541 accggggacg ctactgccat ccgtacagca tggatatcac cgtcacccgg
//
LOCUS       DBBGLQ010000464          586 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.471, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000464 DBBGLQ010000000
VERSION     DBBGLQ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.471"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            154..543
                     /locus_tag="ACIYSB_005877"
     CDS             154..543
                     /locus_tag="ACIYSB_005877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGC5432628.1"
                     /translation="MKKQKFFIVSSILVMCLSTSHISLASGLDKNMQSLARNMSTLQS
                     SGDKKSLLNALDMMDKAVNASMNTLPEKINSNDKVAVESYKNELKKLQNEINLAKEKV
                     ESGNIESLRDALDKMNNIKLEGHRKFR"
BASE COUNT          232 a           84 c          100 g          170 t
ORIGIN      
        1 aatcattcag caataccgaa taaagtagaa tagtttttag aaatgcatca cattttcttg
       61 tgtataccca tataggagat gtacatccat aaaagataga ataataaagt taaattgcca
      121 tgacgataat cactttcgaa taggagtaaa ttaatgaaga aacaaaaatt ctttattgtg
      181 agttctatac ttgtaatgtg tttatctacc agtcatatat ctttggcttc cggactggat
      241 aaaaatatgc agagccttgc aagaaatatg agcactctac aatcctctgg tgataaaaaa
      301 tctctattaa atgctcttga tatgatggac aaagctgtaa atgcttccat gaatactctt
      361 cctgaaaaaa taaacagcaa cgataaagtc gcagtggaaa gttataagaa tgagttgaag
      421 aaactacaaa atgaaatcaa tcttgcaaaa gagaaggttg agtcggggaa tatagagtca
      481 ctacgtgatg ctctggataa aatgaataat ataaaactgg aaggccaccg aaaattcaga
      541 taaataatat tctatagaaa aatttaattt tatttctaaa tacggc
//
LOCUS       DBBGLQ010000465          578 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.472, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000465 DBBGLQ010000000
VERSION     DBBGLQ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.472"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..123
                     /locus_tag="ACIYSB_005878"
     CDS             <1..123
                     /locus_tag="ACIYSB_005878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HGC5432629.1"
                     /translation="STETYTGREVQDIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            138..578
                     /locus_tag="ACIYSB_005879"
                     /pseudo
     CDS             138..578
                     /locus_tag="ACIYSB_005879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
BASE COUNT          132 a          147 c          172 g          127 t
ORIGIN      
        1 agcactgaaa cgtataccgg tcgtgaagtg caggatattc ccggtgtgct ggctgtgttt
       61 gcagaacgtc gtaaggacag ttttggcccg tatgtccggc tgatgagcgt caccctgaac
      121 tgagtcaggg cattctgatg agcagaatta tcaccatcac agtgtatacg ctggatgaac
      181 tttcctgccc ggcacgggaa aaagcccgcg acgggtaccg tcagcatcat gctgacagca
      241 actggtatga aaacgtgtat gaggacgtca gggaggtctg tggcattttc ggaattgacc
      301 tctgccagcg tgttatccgt ctcagcaacg gcaggtttat ggaagagccg tgcatctggt
      361 tcagcggatt ctgcagccag ggacggagcc tgttttgaag gacgctggca ctggcagtca
      421 gcaacaacca ggagagtccg ggagtatgcc ccgcagaacc gtgagcttca tcgtatcgcg
      481 gatgccctgc aggcggtgca gaaacgtaac ttctggcaac tgcaggctga aatccaccac
      541 cggggacgtt actgccatcc gttatcactg ttacccgg
//
LOCUS       DBBGLQ010000466          577 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.473, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000466 DBBGLQ010000000
VERSION     DBBGLQ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.473"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..141
                     /locus_tag="ACIYSB_005880"
     CDS             <1..141
                     /locus_tag="ACIYSB_005880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HGC5432630.1"
                     /translation="MSLLETAKRNGLEPHAWLTDVLTRLPEWPEDRLAELLPLEGFTF
                     SG"
     gene            complement(487..577)
                     /locus_tag="ACIYSB_005881"
                     /pseudo
     CDS             complement(487..577)
                     /locus_tag="ACIYSB_005881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491456.1"
                     /note="with SitACD is involved in the transport of
                     manganese and iron; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese/iron ABC transporter ATP-binding
                     protein"
BASE COUNT          121 a          182 c          160 g          114 t
ORIGIN      
        1 atgagtcttc tggaaacagc aaagcgtaat ggtctggagc cacatgcctg gctgacggac
       61 gtactgacgc gcctgccgga gtggcctgag gaccgactgg ctgagctgct gcctcttgag
      121 ggatttacct tctccgggtg acacaacacc tgccgtcagg tctttgcaca ccgggccatg
      181 gcctgctgtc gggcgttgag cgcctgacag cgggttattg agaaagtcag tcaccatgcg
      241 cctggctgac agtcaggacc tgcacgtcag cgcctccctt accctggtca ggacttcact
      301 gcctgccaca aactcccgtg tcagtacttc gcggcctgac gcgctcaccc tactcagtcc
      361 ttcagagccc gcctccggta cctggcaagg cagacgccgg ggcgcaataa aataaatcaa
      421 ccatcgcagg gcggcgatca ttcgcaacaa ccgctgatga cgagcccaga aacgtgtcaa
      481 gacgatttca ccggaaccgt ttaccccaag cagagcggca atagatccgc ctggtactgt
      541 gaaggatgca tcgcgcagtg ctttctgccc gtttcgc
//
LOCUS       DBBGLQ010000467          577 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.474, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000467 DBBGLQ010000000
VERSION     DBBGLQ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.474"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            201..>577
                     /locus_tag="ACIYSB_005882"
     CDS             201..>577
                     /locus_tag="ACIYSB_005882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HGC5432631.1"
                     /translation="MITKRERRFLRTVEWLGNALPHPVILFIILTISLIVVSMIGQYF
                     NISAPDPRPPGAKGRNDDGIIYIVSLLDADGVRKILSNLVTNFTGFAPLGTVLVSLLG
                     VGIAERSGLISATLRLIVTKAPA"
BASE COUNT          187 a           95 c          106 g          189 t
ORIGIN      
        1 caaaaatatt tattataggt attatgaaat aacctttcca tattgtttat tttaatgcaa
       61 tcagcacaga ttacttcaaa aatataatta gatgcatgaa aagggagaaa caatatattt
      121 tattgtttaa gatattttgt tcaacatgta tgctgagaga taaacttatc gatacttaaa
      181 aaatccgaaa aggaggaata atgataacaa aaagagaaag acgtttcctg agaacagtag
      241 aatggcttgg caatgcactt cctcatcccg tcatcctgtt cataatatta acaatttcct
      301 taattgtagt ttcgatgatt ggacaatatt ttaatattag cgccccagac ccaagaccac
      361 ctggagctaa aggccggaat gatgatggta ttatttatat tgtaagttta ctggatgcag
      421 atggagttcg taaaattctt tcgaatctgg ttactaattt tacgggattt gcaccactgg
      481 gcactgttct ggtttctctt cttggtgttg gtattgcaga acggtccggg ttaatttcag
      541 caacccttcg tcttattgtg acaaaagccc ctgcaaa
//
LOCUS       DBBGLQ010000468          577 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.475, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000468 DBBGLQ010000000
VERSION     DBBGLQ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.475"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>577)
                     /locus_tag="ACIYSB_005883"
     CDS             complement(<1..>577)
                     /locus_tag="ACIYSB_005883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5432632.1"
                     /translation="GVYGENFTLNNNGSVWGGDGYNGGIAVSGNKISINNYRNVYGGN
                     GLGGSGSSGGAGLSGDDIIVDNYRSIYGGDDVGGTGGSGVTGSNITVHNSGGILGGNG
                     VNGGDGINGSNLFITNDNMISGGYGIKQGGDAISGNQITLNNNGIVQGGYGPDGGCSV
                     YGEDIHINNHGNLSGLYNSQKDAYNTSIIFSG"
BASE COUNT          170 a          159 c           66 g          182 t
ORIGIN      
        1 ccagaaaaaa ttattgatgt attataagca tctttttggc tattatataa tcctgaaaga
       61 ttaccatgat tattaatatg gatatcttct ccataaacag agcaaccacc gtcggggcca
      121 tatcccccct gaacaatacc gttattattc aaagtgattt gatttccaga aatagcatct
      181 cccccttgtt ttattccata tcctccagat atcatgttgt cgttagtaat gaaaagatta
      241 ctaccattaa taccatcacc accgtttacg ccattaccgc ccaatattcc tccggaatta
      301 tgcactgtaa tattgctacc ggttacaccg gaaccacctg tcccacctac atcatcacct
      361 ccgtatatac ttctgtaatt atcaactata atatcatccc cgcttaaccc tgcacctccg
      421 ctacttcctg agccaccaag accattaccc ccatatacat ttctgtaatt gtttatagat
      481 attttgttgc cactaacagc aattccacca ttatatccat ctcctcccca aacactacca
      541 ttattattta gcgtaaagtt ttcaccgtag acacctg
//
LOCUS       DBBGLQ010000469          571 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.476, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000469 DBBGLQ010000000
VERSION     DBBGLQ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.476"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..165
                     /locus_tag="ACIYSB_005884"
     CDS             <1..165
                     /locus_tag="ACIYSB_005884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein F"
                     /protein_id="HGC5432633.1"
                     /translation="VATMALYADQWLEEAKKRVKRIGLIITGISGGFVFFFILNMISA
                     IYSISDMLKQ"
     gene            216..>571
                     /locus_tag="ACIYSB_005885"
     CDS             216..>571
                     /locus_tag="ACIYSB_005885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HGC5432634.1"
                     /translation="MKFRSCLYSYFALKKEKEEDKGLTLLEVAGALIIIGIVVVLAMS
                     QMSSTMSSSDNQREVKNLQTIATKMKQYKFQGQYTSSDYIKTLTQSGSLPADMIAGGN
                     KAKNAWGGDVTIKATA"
BASE COUNT          180 a          115 c          129 g          147 t
ORIGIN      
        1 gtggcaacca tggcactcta tgccgatcag tggctggagg aagcaaaaaa acgggtaaaa
       61 cgaattggat taattattac cggtatatca ggaggttttg tcttcttttt tattcttaac
      121 atgatatctg ccatctacag tatcagcgac atgctgaagc aataattccc ccatacagaa
      181 tatttctgaa acatcccata aaccaggagt gatatatgaa attcagatca tgtctttatt
      241 cttattttgc tttaaaaaaa gagaaagagg aggataaagg tttaacctta ctggaagttg
      301 ccggcgcact gattattatc ggtattgtgg ttgttctcgc aatgagtcag atgtccagta
      361 ccatgtcatc cagtgataac cagcgcgaag tgaaaaacct gcagaccatt gcaaccaaaa
      421 tgaagcagta caagttccag ggacaatata ccagcagtga ttatatcaaa acgctgacgc
      481 agagtggttc cctgccggcc gatatgatcg ccggtggtaa caaggccaaa aatgcctggg
      541 gcggtgatgt caccattaag gcaacggcag a
//
LOCUS       DBBGLQ010000470          569 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.477, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000470 DBBGLQ010000000
VERSION     DBBGLQ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.477"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            66..263
                     /locus_tag="ACIYSB_005886"
                     /pseudo
     CDS             66..263
                     /locus_tag="ACIYSB_005886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
BASE COUNT          150 a          103 c          135 g          181 t
ORIGIN      
        1 agacttgtta agtggtcgtt tcagggatat cgttaagact tgttaaatag tctcaaaggt
       61 gcgttatgcc taacatcatt ctgtctgaca ccagtgcaag tgtcagtgaa ctgaaaaaaa
      121 atcctatggc tacagtcagt gccggagatg gttaccctgt tgccattctt aaccggaatc
      181 agccggcatt ctactgtgtg cctgctgaac tgtatgaaag gatgcttgat gctctggatg
      241 accaggaact ggtgaaactg gttactgggg tcagtttgga tatcgaaaat tattgtacgt
      301 taagctcatt ttttatcatt tctggttttt ttggaaacgt aatattctgg atgttaagac
      361 ggtcatgcgg ggacattgaa cacagataat catgtccagg tacgaacgtc ccatatagag
      421 tgtgagcatt gatcgtgctg tattttcgtt tatagtagat agattctgta tcatacagag
      481 cacgtggtca gcactaaagt acattacgct gacttattct gttcactgag ggctgagtac
      541 ctgctttcaa ggatttatct gctgaatca
//
LOCUS       DBBGLQ010000471          560 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.478, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000471 DBBGLQ010000000
VERSION     DBBGLQ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.478"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT          202 a          110 c           92 g          156 t
ORIGIN      
        1 tgcaggtatc aggaagatct caggatatga agattcccac agcacagatt taaaattagt
       61 cgaatataat aaatttaaaa accttaatta aaatgtaatc tcatccatgg ttaacatgct
      121 cctgaaaagg aaagttacaa ataccgtatg ctaccaacta attacaacaa tgatatatgt
      181 acattcagta agaatacaga taaaatatta ataccaactt ccagtatcaa gatcgtatcg
      241 cgaacttcag atgcgctatg aggcagttca atttttaact ttcgttttcc tcttcctccc
      301 tggcttcata tgcataagcg acttgaaaga cccgatgata gttacaattt caggttgtgg
      361 catgacgatc aatttgtaac tgaaacaatg aacggtaacg ctgccatata aacatcaaaa
      421 tgcgagcatt acagcgacat tgaaccgatt agatgtatca ctcataatca gaacaagatg
      481 atgtatcctg agatataaga aaatcatcaa gagaacctcc ggcttttatt gcttctgcaa
      541 taacattagg ccgacgacct
//
LOCUS       DBBGLQ010000472          558 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.479, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000472 DBBGLQ010000000
VERSION     DBBGLQ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.479"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..534
                     /gene="sitA"
                     /locus_tag="ACIYSB_005887"
     CDS             <1..534
                     /gene="sitA"
                     /locus_tag="ACIYSB_005887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HGC5432635.1"
                     /translation="PHAWMSPDNALIYVDNIRDALIKYDPANAKTYQRNADTYKAKIT
                     QTLAPLRKQIAELPENKRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKV
                     VDIVKKNNIPAVFSESTVSDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLK
                     VTTSTLVQGIKAGKREK"
BASE COUNT          163 a          125 c          139 g          131 t
ORIGIN      
        1 cctcatgcct ggatgtcgcc agataatgct ttgatttacg tcgataatat tcgtgatgcg
       61 ttgataaaat acgatccggc aaatgcaaaa acataccaac gtaatgccga tacttataaa
      121 gccaagatta cccaaaccct tgcccccctg cgtaagcaga ttgctgaact ccctgagaat
      181 aaaagatgga tggtcaccag tgaaggggct ttttcttatc tcgcacgcga tttggggcta
      241 aaagagcttt atctgtggcc gataaatgcc gatcaacaag gaacacccca gcaggtacgt
      301 aaagtggttg atatagtgaa gaaaaataat atcccagcgg tatttagtga gagtacggtt
      361 tccgataaac cagcgcgtca ggttgcgcgt gaaaccggcg cacactacgg cggtgtgctc
      421 tatgtcgatt ccctgagcac agaaaacggc ccggtaccta cgtatatcga ccttctgaag
      481 gttactacca gtacgctggt gcagggaatt aaagccggga aaagggagaa gtaatgatgc
      541 aatctgcggg tattgtcg
//
LOCUS       DBBGLQ010000473          553 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.480, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000473 DBBGLQ010000000
VERSION     DBBGLQ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.480"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..552)
                     /locus_tag="ACIYSB_005888"
     CDS             complement(<1..552)
                     /locus_tag="ACIYSB_005888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGC5432636.1"
                     /translation="MELDKRPVKLDERETFNVSWGIDENYQVGAAISIASILENNKQN
                     KFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQSNIWPVSIYYRLL
                     SFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNE
                     DFNGSYFNSGVMYINLREWLKQRL"
BASE COUNT          186 a          110 c           78 g          179 t
ORIGIN      
        1 tagtctttgt tttaaccatt cccgtaaatt aatatacatt acaccagagt taaaatagct
       61 accgttaaaa tcctcattac acaaacgctc agcgctttta ctttgcatag catctacatc
      121 aattaccact gccccatatt catctttaaa ctctaatgct attaactcgt tcaatgaacc
      181 cttacagacg atatcagcat caagatataa taatgaatcc aatcgcgcag aaaaataatc
      241 aaatgaaagc aaacgataat aaatagatac tggccagata tttgattgag gtagcgcctt
      301 caatggctca gaatcaatat gatataattt aattactgtt tgatacttcg ttgctaattg
      361 tgataataat tcaatatact ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt
      421 gttttgttta ttattttcaa gaattgaagc aattgaaatc gcagccccaa cctggtagtt
      481 ctcatcaata ccccatgaga cattaaacgt ttcccgttca tcaagtttaa cggggcgctt
      541 gtccagttca att
//
LOCUS       DBBGLQ010000474          549 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.481, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000474 DBBGLQ010000000
VERSION     DBBGLQ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.481"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>549
                     /locus_tag="ACIYSB_005889"
     CDS             <1..>549
                     /locus_tag="ACIYSB_005889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HGC5432637.1"
                     /translation="YENISSFIRDVFIHSEENDLIPDFLNSTFVDWDDAKYMTESMSF
                     VLEEVSVILNKENTEKTEISYDQNLYSLLAHHNHITPCWNNVISLLSEDASLAGDTFC
                     KWLNINYSLLPNDSLPLTDVQFSQLLIKAVTSPHISKEALIAITMAFRITLINVPENL
                     PLNNAAVLIKQKWLAPTSTVFEQ"
BASE COUNT          177 a          101 c           93 g          178 t
ORIGIN      
        1 tatgaaaaca tatcatcatt cattcgagat gtttttatac actctgaaga aaatgatctc
       61 attcctgatt ttcttaattc gacattcgtt gactgggatg atgcaaagta catgacagaa
      121 agtatgagct tcgtactgga agaagtatca gttatcctga acaaagagaa taccgaaaaa
      181 acagaaattt cttatgatca aaacctgtat tcacttctgg ctcatcataa tcatattacc
      241 ccttgctgga ataatgttat ttctctactg agtgaagatg cgtcacttgc tggtgatact
      301 ttttgtaagt ggcttaatat taattattct ttattgccaa atgactcgtt gcctttgact
      361 gatgttcagt tctcgcaatt acttattaag gctgttacat cacctcatat cagtaaagaa
      421 gcgttaatag cgataaccat ggcatttagg ataactctta ttaatgtacc agaaaactta
      481 ccactgaata atgctgcggt actaatcaag caaaaatggc tggcaccaac cagtactgtg
      541 tttgaacaa
//
LOCUS       DBBGLQ010000475          540 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.482, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000475 DBBGLQ010000000
VERSION     DBBGLQ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.482"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            95..>540
                     /locus_tag="ACIYSB_005890"
     CDS             95..>540
                     /locus_tag="ACIYSB_005890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGC5432638.1"
                     /translation="MAKKSAEQSMAIAEVDRKVDRMGSEIRQTVSRSTEENSRQIAQI
                     RQYVDDKGSQIKQSTDKKLGSWNASIEQIQKVQEDTRRNLNSMWAVKLQQTRDGRLYI
                     AGIGAGIENTSDGIQSQILMQADRIAMINPENGNTTPLFVAQGDQL"
BASE COUNT          175 a          101 c          167 g           97 t
ORIGIN      
        1 gcagacagtg agcaggagca cggaggagaa cagccgacag atagcgcagg tcaggcagta
       61 tgttgatgat aaaagtgcgc aaataagtac tgttgtggcg aaaaagagcg ctgaacagtc
      121 aatggccatt gcggaagttg acagaaaagt tgacaggatg ggaagcgaaa tccggcagac
      181 agtaagcagg agcacggagg agaacagccg acagatagcg cagatcagac agtatgttga
      241 tgataaggga agtcagatca aacagagcac ggataaaaaa ctgggatcct ggaatgccag
      301 cattgagcag atacagaaag ttcaggagga cacgcgccgg aacctgaaca gcatgtgggc
      361 tgtgaagctg cagcagacac gggatggacg tctctatatt gcaggtattg gtgccggaat
      421 cgaaaatacg tctgacggaa ttcagagtca gatcctgatg caggcagatc gtattgcgat
      481 gattaacccg gaaaatggca atacaacacc tttgtttgtg gctcagggtg atcagctgtt
//
LOCUS       DBBGLQ010000476          540 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.483, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000476 DBBGLQ010000000
VERSION     DBBGLQ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.483"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            160..>540
                     /locus_tag="ACIYSB_005891"
     CDS             160..>540
                     /locus_tag="ACIYSB_005891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415558.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HGC5432639.1"
                     /translation="MFNEVHSIHGHTLLLITKSSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMEDQERVVNGLQGVLRG"
BASE COUNT          159 a          105 c          108 g          168 t
ORIGIN      
        1 tagattgaaa tcagatgtaa tccattagtt ttatatttta cccatttagg gctgatttat
       61 tactacacac agcagtgcaa catctgtcag tacttctggt gcttctattt tagaggcagc
      121 tgtcaggtgt gcgatcaata aaaaaagcgg ggtttcatca tgtttaatga agtccatagt
      181 attcatggtc atacattatt gttgatcact aaatcttctt tgcaggcgac agctctcttg
      241 cagcacctta aacaatcgct ggcaattaca ggaaaattac ataatattca acgttctctg
      301 gacgatatct cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
      361 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      421 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      481 ttttattcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
//
LOCUS       DBBGLQ010000477          528 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.484, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000477 DBBGLQ010000000
VERSION     DBBGLQ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.484"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            149..>528
                     /locus_tag="ACIYSB_005892"
     CDS             149..>528
                     /locus_tag="ACIYSB_005892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432640.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQHNKTWRHVKNERINRSASWQYIG
                     AGDDQITLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVITAVRTTR
                     SELDRHGKAKKIEFTVSLERCDED"
BASE COUNT          131 a          129 c          145 g          123 t
ORIGIN      
        1 gcgaactgca tattcacctg catgatgttc acacgcaaaa cccgcgcgaa ctggcaaaaa
       61 tggtgggtga tgccgtccgt gcggaaatgg aacgccgtat ccgccagaca gtcggtagtt
      121 tccgtgacag agattaggag tacctcgcat gatgatgatt tacggcatgt ttgtttttga
      181 gttgcgtacg ctgcctcatc aacaactgca acataataaa acctggcgcc acgttaaaaa
      241 tgaacgcatc aatcgttcgg ccagctggca gtatatcggg gcgggtgacg accagatcac
      301 gttgtccggt gtgctttatc ccgaaattac cggcggtgag gtttccctca gccttttaac
      361 gacgcaggct tacactggtc gcccatggcc gctgattgac ggtgtggggc agatatacgg
      421 catgtatgtg ataacggctg tccgcacaac gcgaagcgaa cttgaccgcc acggtaaggc
      481 caaaaaaatt gagttcactg tcagtctgga acgctgtgat gaggattt
//
LOCUS       DBBGLQ010000478          527 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.485, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000478 DBBGLQ010000000
VERSION     DBBGLQ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.485"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>527
                     /locus_tag="ACIYSB_005893"
     CDS             <1..>527
                     /locus_tag="ACIYSB_005893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGC5432641.1"
                     /translation="VDLSQLPEPAIIAQPDFESILADIKTMMVSSFPEEYRAAVTRAL
                     TLESEPLNIISQAMAFREMLLRQRINEAARACMLSHSRGSDLDNLAANNNTRRLVIHP
                     ATDTTEAVMESDTSLRLRAQSAWDGLSVAGPSGAYEYFARSASGLVRDARAISPSPAT
                     VTVSILSADGDGTAG"
BASE COUNT          105 a          180 c          144 g           98 t
ORIGIN      
        1 gttgacctgt cacagttacc ggaaccggcc atcatcgcgc agccggattt tgagtccatc
       61 ctggcagaca ttaaaaccat gatggtgagc tccttcccgg aagaataccg cgccgccgtc
      121 acccgtgccc ttactctgga atccgagcca ttgaacatca tttcccaggc catggccttt
      181 cgtgaaatgc tgcttcgcca gcgcatcaat gaagccgccc gcgcctgcat gctcagccac
      241 agccggggca gtgacctgga caatctggcc gccaacaaca acacccgccg cctggtcatt
      301 catccggcca ctgataccac ggaagcagtg atggaaagcg acacatcgct gcgtctgcgt
      361 gcccagtcag cctgggacgg cctgagtgtg gccggaccgt ccggcgcgta tgagtatttt
      421 gcccggagcg caagcggtct tgtccgtgat gcgcgcgcca tcagcccgtc accggccaca
      481 gtcaccgtct ccatcctgtc agcagacggt gacggcaccg ccggtga
//
LOCUS       DBBGLQ010000479          516 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.486, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000479 DBBGLQ010000000
VERSION     DBBGLQ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.486"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            51..218
                     /locus_tag="ACIYSB_005894"
     CDS             51..218
                     /locus_tag="ACIYSB_005894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432642.1"
                     /translation="MTVRYLNFQIKNITGGCYDWFVTLGKEVITGKLDEVKAKAMAYA
                     CKQARKKSAKA"
     gene            342..461
                     /locus_tag="ACIYSB_005895"
                     /pseudo
     CDS             342..461
                     /locus_tag="ACIYSB_005895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
BASE COUNT          142 a          133 c          119 g          122 t
ORIGIN      
        1 caccactcac gcgataatct gcattcttta ctcgcgccgg gggtgtcaaa atgactgttc
       61 gctacctcaa ctttcaaatc aagaacatta ctggcggttg ctatgactgg tttgtcactc
      121 tcggaaaaga agtgatcacc gggaagctgg atgaagtgaa agctaaagca atggcctacg
      181 cctgcaagca agcccggaag aaatccgcca aagcataaaa tactgacttt tgcgccctgg
      241 cattatcgtg gggtatattt ctacggcacc ttagaaaaac gggtgccggg attggaacct
      301 cacgcggtag ctccttaact tagtcaaagg aggctgccaa tatcgctact atccccaccc
      361 caactcattc tgaatttatc tggcgtttct attcctgcca aaaacacctg tatatctgcg
      421 tcatggctgc aaccgaagca gaagctcgtt cataccttcc cctggcggaa cggggcgtaa
      481 ttggtgacaa acagatactg ggaaacagtg gaacta
//
LOCUS       DBBGLQ010000480          512 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.487, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000480 DBBGLQ010000000
VERSION     DBBGLQ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.487"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>512
                     /locus_tag="ACIYSB_005896"
     rRNA            <1..>512
                     /locus_tag="ACIYSB_005896"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          122 a          115 c          169 g          106 t
ORIGIN      
        1 ctcctaaaga gtaacggagg agcacgaagg ttggctaatc ctggtcggac atcaggaggt
       61 tagtgcaatg gcataagcca gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc
      121 aggtcatagt gatccggtgg ttctgaatgg aagggccatc gctcaacgga taaaaggtac
      181 tccggggata acaggctgat accgcccaag agttcatatc gacggcggtg tttggcacct
      241 cgatgtcggc tcatcacatc ctggggctga agtaggtccc aagggtatgg ctgttcgcca
      301 tttaaagtgg tacgcgagct gggtttagaa cgtcgtgaga cagttcggtc cctatctgcc
      361 gtgggcgctg gagaactgag gggggctgct cctagtacga gaggaccgga gtggacgcat
      421 cactggtgtt cgggttgtca tgccaatggc actgcccggt agctaaatgc ggaagagata
      481 agtgctgaaa gcatctaagc acgaaacttg cc
//
LOCUS       DBBGLQ010000481          501 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.488, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000481 DBBGLQ010000000
VERSION     DBBGLQ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.488"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..135
                     /locus_tag="ACIYSB_005897"
     CDS             <1..135
                     /locus_tag="ACIYSB_005897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGC5432643.1"
                     /translation="GRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPSDHFWQP
                     "
BASE COUNT          157 a          119 c          101 g          124 t
ORIGIN      
        1 ggtcgttacc ttctgctgtt cagggtactg gatactgaag ttcgtatcga acgtatcgtt
       61 catggttccc gggatatcaa cagggtactt gcagagccag acgacaaacc atcagatcat
      121 ttctggcagc cataaacaaa atctgtgcgt taacgctccc taacacaggt agccaggtat
      181 tttataaatc tctgtatttt gttgttactt cgattccgcg attcagcata tcgaaccaga
      241 caaaatgatt acaacaaagc acaaaaaaga gggattatag cgtcaaaaga attcgaattg
      301 cacatggggg cctcattggt ggccccaaaa agaacaaaga caaaacagtc gatacaaaac
      361 aattggttat ataaccagtt tcattccgag tccgggcacc aaattcatat aaacggacct
      421 ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgtcctgc cgccaaatca
      481 attctaccga actcaaccag a
//
LOCUS       DBBGLQ010000482          499 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.489, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000482 DBBGLQ010000000
VERSION     DBBGLQ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.489"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..268
                     /locus_tag="ACIYSB_005898"
     CDS             <1..268
                     /locus_tag="ACIYSB_005898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGC5432644.1"
                     /translation="TYQYMDLKPEGYKDRNAVNGSFYKLTFAPTLKAGGLGDFFSRPE
                     LRLFATWMDWSSRLDNYASDDAFGGSGFNAGGEWNFGVQMETWF"
BASE COUNT          130 a          114 c          132 g          123 t
ORIGIN      
        1 cacttaccag tacatggatc tgaaacctga agggtataag gaccgcaatg ctgttaacgg
       61 cagtttttac aagctgacgt ttgcgcccac cctcaaggca ggaggtctgg gggacttctt
      121 cagccgtccg gaactgcgtc tgttcgccac ctggatggac tggagcagca gactggataa
      181 ctacgccagt gatgatgctt tcggcggtag cgggtttaat gcaggcgggg aatggaattt
      241 tggcgttcag atggaaacct ggttctgaac actaacgctc ccggtgtacc gggagcgttg
      301 ccggtcattg tcccgtaaat gaaagcgttg ttcatcaata ggaatttaaa ccccaaaaag
      361 attaaaaaaa cacctgaaaa agggcgcttt ctcagcatcg tcacacatgc cataacttcg
      421 acgtcatcaa ttaaatggac ttcattgatt gataccgggt atcgtgacgg gagcctgttt
      481 aggattctgt gtaaatgcc
//
LOCUS       DBBGLQ010000483          489 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.490, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000483 DBBGLQ010000000
VERSION     DBBGLQ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.490"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>489
                     /locus_tag="ACIYSB_005899"
     CDS             <1..>489
                     /locus_tag="ACIYSB_005899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5432645.1"
                     /translation="LNRGFTVYTPDTQVQEKDGSVYWQLKSHAGTAESQVSTDVSDDV
                     TDTTSPVAPDTGSTGSTEADGIVSEENNSNGVTPSPDSPAENAGTTVNGSTLFKGADN
                     TSLLKKARAMFAAREFILSDSADRWTQVVDNSDADGGAWAMTGYSHGGYDDFSLNQSG
                     LNV"
BASE COUNT          130 a          110 c          148 g          101 t
ORIGIN      
        1 ctgaacaggg ggtttactgt ttatacccct gacacacagg tgcaggagaa agacggcagt
       61 gtttactggc aactgaaaag tcatgccgga acagcagaat ctcaggtatc aacggatgtc
      121 agtgatgatg taacagatac aacctctccg gttgcaccgg atacagggag tacaggaagc
      181 actgaggctg atggtattgt gtctgaagaa aataacagca acggtgtgac gccttcccct
      241 gattctccgg cagagaatgc gggcacgacg gtgaatggca gtacgctgtt taagggcgcg
      301 gataacacat ctcttctgaa gaaagcgcgt gccatgtttg ccgcccgtga gttcattctc
      361 agcgacagtg ctgaccgctg gacacaggtg gtggacaaca gtgatgcaga cggcggtgca
      421 tgggccatga cagggtacag tcatggtgga tatgacgact tcagcctgaa ccagagcgga
      481 ctgaatgtg
//
LOCUS       DBBGLQ010000484          486 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.491, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000484 DBBGLQ010000000
VERSION     DBBGLQ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.491"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>486)
                     /locus_tag="ACIYSB_005900"
     CDS             complement(<1..>486)
                     /locus_tag="ACIYSB_005900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGC5432646.1"
                     /translation="GLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKVDALAAGLLS
                     VAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVRRNWVIKLPDSV
                     PASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLASLGSVVGWAY
                     VI"
BASE COUNT          144 a          136 c          107 g           99 t
ORIGIN      
        1 gatcacatag gcccagccca ccacgctacc caacgatgcc agtggggttg agatggtatc
       61 cataatgatc tgatggaagt tggtgcccca ggtattcaac gcccaggcaa taatccccat
      121 cacggaaaga ataataaagc cgggaattaa tgccgagaag gaacgcgata ctgaagcagg
      181 tacgctgtcg ggcagtttaa tgacccaatt gcggcggaca ataaaggtaa acatttctgc
      241 caccaccagg ccaataataa tcccggagat gatattcgcc ccacctaacc agtttgcacc
      301 aaccgcatag gcctcaccga cactatatgg ggtgacggtc ataaatgctg caacggataa
      361 caacccagcc gccagcgcat cgactttacg ctcttctgcc agcgccatgc caataaagaa
      421 cggtgccatt aaagacatta ttcctaatgt tccgttatat acgttgccgc caataccttt
      481 cagacc
//
LOCUS       DBBGLQ010000485          484 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.492, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000485 DBBGLQ010000000
VERSION     DBBGLQ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.492"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..165)
                     /locus_tag="ACIYSB_005901"
     CDS             complement(<1..165)
                     /locus_tag="ACIYSB_005901"
                     /inference="COORDINATES: protein motif:HMM:NF012578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGC5432647.1"
                     /translation="MKESTMGLLGRIKQLISENEKPAEVTRVMAPLSGEIVPLDTVPD
                     AVFAGKIVGDG"
     gene            complement(214..>484)
                     /locus_tag="ACIYSB_005902"
     CDS             complement(214..>484)
                     /locus_tag="ACIYSB_005902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HGC5432648.1"
                     /translation="YVDNQAGRLVLWRHYPDEGLDGYRSIALPGAEYLTLRVFLDRSS
                     VEVFVNDGDATLSSRIYPQPGTRRLSLYAAHGEAVLTDGSLWTLA"
BASE COUNT          107 a          154 c          121 g          102 t
ORIGIN      
        1 accatccccc actattttcc cggcaaacac agcatccgga accgtgtcca gagggacaat
       61 ttcaccggac agcggtgcca tcacgcgggt gacttccgcc ggtttctcat tttcacttat
      121 cagttgttta atcctgccca gtaatcccat tgtgctttcc ttcattacgg gtgaaccgaa
      181 agtactgccg tcaccgacgg cagtggaatt atttcaggcc agcgtccaca gtgagccgtc
      241 cgtcagcaca gcctcgccgt gagctgcata tagcgacaac cggcgggttc cgggctgcgg
      301 gtaaatacgg ctggacaggg tggcatcgcc atcgttaacg aacacttcca ccgatgaacg
      361 gtccaggaaa acccgtagcg tcagatattc tgcgccaggc agcgcgatac tgcggtaacc
      421 gtccagcccc tcatccgggt agtgacgcca aagcaccagc cgtcctgcct ggttatccac
      481 atac
//
LOCUS       DBBGLQ010000486          481 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.493, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000486 DBBGLQ010000000
VERSION     DBBGLQ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.493"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>481)
                     /locus_tag="ACIYSB_005903"
     CDS             complement(<1..>481)
                     /locus_tag="ACIYSB_005903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGC5432649.1"
                     /translation="YLDADIACKGSIKELLDYQFSTNEIAAVVAERDVEWWQNRASVL
                     TTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNG
                     KVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIA
                     "
BASE COUNT          153 a           99 c           74 g          155 t
ORIGIN      
        1 gcgatcaaaa aactacgtga caccggatag tcagcccact catgccaggg ttttgtaggt
       61 cccacatagt gtataaacac ggtgtcatca ttgactggat tatcaacttt gtcttttaat
      121 tcatagttaa tactatatcg ggtattatat ttctccgaaa taaacttcac tttaccattc
      181 aataatacat tcagtacatc ttgatcaagg tgggtgattt tacttaccca atctgggtca
      241 cgcaacattt caatagcttt tgacgaaatg ttatttagat tccactcatc aatatttatc
      301 agtaaaaaac cagcattgaa atatccagaa gctaactgtg gtgtagttaa taccgaggct
      361 cgattttgcc accattcaac atctctttca gctacaactg cggcaatttc attagtagaa
      421 aattgataat ctaagagttc tttaatacta cccttgcaag caatatctgc atcaagatat
      481 a
//
LOCUS       DBBGLQ010000487          481 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.494, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000487 DBBGLQ010000000
VERSION     DBBGLQ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.494"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..317)
                     /locus_tag="ACIYSB_005904"
     CDS             complement(<1..317)
                     /locus_tag="ACIYSB_005904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGC5432650.1"
                     /translation="MNSIFTEENLLAFTMAARYRSFSKAAKELGLTTSAISYTIKRME
                     MGLDVVLFTRTTRSIELTEPGHYLFCKATDLLNDYHSIIRRVDAIAQGIKSRVRICIN
                     QLL"
BASE COUNT          139 a          103 c           96 g          143 t
ORIGIN      
        1 tacaacagtt gattaataca aatgcgcacc cgggatttaa ttccctgagc gatggcatca
       61 actctacgta tgatggagtg gtaatcgttg agcaggtcag ttgctttgca aaaaaggtag
      121 tgcccgggct cggtcagctc aatgctacgg gtagttcggg taaaaagcac cacgtcaagc
      181 cccatttcca tacgcttgat agtgtaacta attgccgatg tggttaagcc cagttccttc
      241 gccgccttgc tgaaacttct atagcgggca gccatagtaa atgccagtaa gttttcttcc
      301 gtaaatattg aattcatctt tctttcctct ggcgacaata cacatacaca acatgatgat
      361 tgttgaatgt atttgtaatt cttataaccg acaatcagtt ttgaaaaaat taacaccgta
      421 tttacattta atttgaagga aatcacacat ctgaatttta attacaggca gctatccgtg
      481 c
//
LOCUS       DBBGLQ010000488          472 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.495, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000488 DBBGLQ010000000
VERSION     DBBGLQ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.495"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT          176 a          113 c           85 g           98 t
ORIGIN      
        1 cagggcactg tcgagaaggc tgtcaaaacg ggataaccag taagcattat gtattggttg
       61 attcatgatg agagatctcc gttgatttgg attgacttca catcattaat caccgcagta
      121 aaactttacg gcgtcctgtc attaagcaac accagtggct cctgattaga acagaatgag
      181 tttcccacga acaaaaagac aaacctacac gcagggtaaa aacacgcaac aacagcatga
      241 atggatttga aaacaatatg gatgacgaaa aatgtcaaaa gtgaaacgga tccacagaac
      301 gtgcaaacaa aacatcaaaa acgcaatcag agcactttct ggcgctgtga atcaacaaca
      361 acaccaaaac ctacagctta accacattac tcaatcacga atgcgaatga cacgtttaaa
      421 acctgtttac agataacgcc gtaacacgat catccccatc accatcacca tc
//
LOCUS       DBBGLQ010000489          472 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.496, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000489 DBBGLQ010000000
VERSION     DBBGLQ010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.496"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..412
                     /locus_tag="ACIYSB_005905"
     CDS             <1..412
                     /locus_tag="ACIYSB_005905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HGC5432651.1"
                     /translation="DLKFGQKKKVNVKRRREAGKQKQEKSSSREGDYMEGAEGNVYVL
                     RRTYQNEETARRAAAAKWQELQRGAASFSITLARGRADLYPEMHGRVTGFKSEVDNQD
                     WIIARVEHSIDESGFTTQLELEAKIPEWIADSGG"
BASE COUNT          145 a          109 c          145 g           73 t
ORIGIN      
        1 cgatctgaag ttcgggcaaa agaaaaaagt gaacgtgaag cgacgcaggg aagccgggaa
       61 acagaaacag gagaagagca gcagccggga aggtgattac atggagggcg cggaagggaa
      121 cgtctacgtt ctgcgcagga cttaccagaa tgaagaaacc gcacgacgtg cagcagctgc
      181 aaagtggcag gaactacagc ggggcgcggc atcattctcc atcacgctgg cgcgcggtcg
      241 tgccgacctg taccctgaaa tgcacggcag ggtaacaggt tttaaaagcg aggtggacaa
      301 tcaggactgg attattgcca gggtggagca cagcatagat gaaagcggct tcaccacaca
      361 actggagctg gaagcgaaaa tccccgaatg gatagcagat tcaggcggat aaaattaaaa
      421 tgagccatct ccactcagcc agataaggag aagccatgtt ccgttgtccc ct
//
LOCUS       DBBGLQ010000490          465 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.497, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000490 DBBGLQ010000000
VERSION     DBBGLQ010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.497"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..200
                     /locus_tag="ACIYSB_005906"
     CDS             <1..200
                     /locus_tag="ACIYSB_005906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432652.1"
                     /translation="EFIDYDKEIIKFTHEKLLGEIRRNIQVNLKVNPDNFPELIEIYN
                     SCFSKNILLYDVQDNGFKWCL"
BASE COUNT          168 a           56 c           89 g          152 t
ORIGIN      
        1 gtgaatttat agattatgat aaagaaataa taaaattcac gcatgaaaaa ctgctgggtg
       61 aaattaggcg taacattcag gtgaatttaa aggtgaatcc tgataatttt cctgaattaa
      121 tagaaatata caattcatgc tttagtaaaa atatattgtt gtatgatgtt caggataatg
      181 gttttaaatg gtgtttatga tgtgaaaata ttaaattcat attacatttt aattatcgac
      241 aaaaaacaca taaagaaatc taaagggaag gggtgtcaaa tgagctgata tagcgccagt
      301 ttggtatggt catgtgattt gatgattaaa gcttacagat aataacactc ctctgtgagc
      361 catataaatg agcatgccat aatcgtcagt tcagagataa aactgactga atgatcttat
      421 gttatggtta gcagtgttta ccggatacga tgacttatct ctctc
//
LOCUS       DBBGLQ010000491          464 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.498, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000491 DBBGLQ010000000
VERSION     DBBGLQ010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.498"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..131)
                     /locus_tag="ACIYSB_005907"
     CDS             complement(<1..131)
                     /locus_tag="ACIYSB_005907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432653.1"
                     /translation="MPVGVPIPWPSATPPTGWLKCNGAAFSSEKYPNLARVYPTLKLP
                     "
     gene            complement(160..>464)
                     /locus_tag="ACIYSB_005908"
     CDS             complement(160..>464)
                     /locus_tag="ACIYSB_005908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432654.1"
                     /translation="GRTILSAQGDAIRNIYGEFRTVNTENYSIWESAGTFKGAVVPLS
                     PSTNNSYFSLIRSMVTERTDGTFYPKVIGLDASRIVPTANENRPRNIAFNYIVRAA"
BASE COUNT          116 a          108 c          103 g          137 t
ORIGIN      
        1 ggtaatttta acgttgggta aacccttgcc agatttgggt acttttcaga agaaaatgct
       61 gcaccattgc atttcagcca ccccgttggc ggtgtggctg agggccatgg aataggtaca
      121 ccaacgggca gcgccgagcc ttcccctaaa ccaacgtttt catgcggctc ttacgatgta
      181 attaaacgca atgttgcgtg ggcggttttc gtttgcagtt ggaacaattc ttgaagcatc
      241 aaggccaatc acttttgggt aaaaagtgcc atctgttctt tctgtcacca tacttctgat
      301 taaggagaaa taactattgt tcgttgaggg actcaaaggc accactgccc ccttaaacgt
      361 gcctgctgat tcccatattg aataattttc agtgtttaca gttctgaact caccatagat
      421 attacgtatg gcatcacctt gagcggataa tattgttctc cccg
//
LOCUS       DBBGLQ010000492          461 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.499, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000492 DBBGLQ010000000
VERSION     DBBGLQ010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.499"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..161
                     /locus_tag="ACIYSB_005909"
     CDS             <1..161
                     /locus_tag="ACIYSB_005909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HGC5432655.1"
                     /translation="FPFCTGKYRWHGGTEAYTGREVQDIPGVLAVFAERRKDSFGPYV
                     RLMSVTLN"
     gene            180..>461
                     /locus_tag="ACIYSB_005910"
     CDS             180..>461
                     /locus_tag="ACIYSB_005910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HGC5432656.1"
                     /translation="MSSIITTTVYTLDELSCPAAREKARDWYRQYHADSNWYENVYED
                     FREVCNIFGISLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHW"
BASE COUNT          100 a          111 c          139 g          111 t
ORIGIN      
        1 tttttccgtt ctgcacaggt aagtaccgct ggcacggtgg cactgaagcg tataccggtc
       61 gtgaagtgca ggatattccc ggtgtgctgg ctgtgtttgc agaacgccgt aaggacagtt
      121 ttggcccgta tgtccggctg atgagcgtca ccctgaactg aatcagaaag gggattctga
      181 tgagcagcat tatcaccacc actgtgtata cgctggatga actttcctgc cctgctgcac
      241 gggaaaaagc ccgcgactgg taccgtcagt atcatgctga cagcaactgg tatgaaaacg
      301 tgtatgaaga cttcagggag gtctgtaaca ttttcgggat tagcctccgc cagcgcgttt
      361 tccggctcag caacggcagg tttatggaag agccgtgcat ctggttcagc ggattctgca
      421 gccaggggga cggagcctgt tttgaaggac gctggcactg g
//
LOCUS       DBBGLQ010000493          459 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.500, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000493 DBBGLQ010000000
VERSION     DBBGLQ010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.500"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>459
                     /locus_tag="ACIYSB_005911"
     CDS             <1..>459
                     /locus_tag="ACIYSB_005911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGC5432657.1"
                     /translation="PVMLGSNQVIVEEQGKLSGFGGVAGNLSNSGIVDLTTYMPGNIL
                     TVGGNYTGRNGLILLQTETGGDNSKTDRLVIKGNASGRTRVAVTQAGGTGAETLNGIE
                     VIHVSGNADNAEFIQTERITAGAYDYILKRGQGINSTNWYLISRKDIPVP"
BASE COUNT          129 a           92 c          125 g          113 t
ORIGIN      
        1 caccggtgat gctcggcagt aaccaggtca ttgttgaaga gcagggcaaa ctctccgggt
       61 ttgggggcgt tgcaggaaat ctgagcaata gtggtatagt cgatctcact acatatatgc
      121 cgggtaatat actgactgtc ggagggaatt acactggcag aaatggactt attctcctcc
      181 agacagaaac aggtggtgac aattcgaaaa cagatcgtct ggtgattaaa ggtaatgcca
      241 gtggccgtac ccgtgtcgct gttactcagg ccggtggtac tggtgcagag acacttaatg
      301 ggattgaagt gattcacgtc agtggcaatg ctgataatgc tgaattcatt cagacggaac
      361 gtattacagc cggagcttat gattacatac tgaaacgtgg tcaggggatt aacagcacta
      421 actggtatct gattagcaga aaagacattc ctgtaccac
//
LOCUS       DBBGLQ010000494          454 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.501, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000494 DBBGLQ010000000
VERSION     DBBGLQ010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.501"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>454
                     /locus_tag="ACIYSB_005912"
     CDS             <1..>454
                     /locus_tag="ACIYSB_005912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGC5432658.1"
                     /translation="LLQVLREHFPTCQIAITTEVYNGVWDSLINNQADIAIGAPDTLL
                     DGGGIDYIEIGMIRWLFAISPAHPLAFIPEPISESQLRLYPNIMVEDTANTINKKVGW
                     LLHGQESILVPDFNTKLQCQILGEGIGFLPDYMVREAMEKSLLVTRQIH"
BASE COUNT          111 a          104 c          117 g          122 t
ORIGIN      
        1 cttctgcaag tgctcaggga acactttcca acctgccaga tagctattac tacagaggtg
       61 tataatggcg tctgggattc actcattaat aaccaggctg atatcgccat tggcgcgccc
      121 gatacgttac tggacggtgg tggaattgat tacattgaaa tcggcatgat tcgttggttg
      181 ttcgcgattt ctccggctca tccgctggcg tttattcctg agcccatttc tgagagtcag
      241 ttacgtctgt acccgaacat catggtggaa gacactgcaa acactattaa caaaaaagtc
      301 ggctggctgt tacacgggca agagtcgatt ctggttcccg actttaatac taagcttcag
      361 tgccagatcc tgggggaggg aatcggtttt ctgccagact atatggtgcg tgaagcgatg
      421 gaaaagtcat tgctggtgac acggcagatc caca
//
LOCUS       DBBGLQ010000495          452 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.502, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000495 DBBGLQ010000000
VERSION     DBBGLQ010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.502"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>452
                     /locus_tag="ACIYSB_005913"
     CDS             <1..>452
                     /locus_tag="ACIYSB_005913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432659.1"
                     /translation="FLMELEDAPYYPKALSELEAVVQSILHRAESAASEAEEARKRAA
                     SARGPRGEKGDTGARGERGITGPQGPKGETGARGPAGPQGPKGETGERGPAGPQGAKG
                     DTGARGPAGPQGPKGETGERGPEGPQGPKGETGERGPAGPQGAKGETG"
BASE COUNT          128 a          109 c          165 g           50 t
ORIGIN      
        1 aatttctgat ggagctggaa gatgctcctt attatccgaa ggcgttgtca gagctggagg
       61 ctgtggtgca gtccatactg cacagagcag agtccgcggc atctgaggcg gaggaagccc
      121 ggaaacgagc tgcatctgcc cggggaccac gaggagagaa aggggatacc ggcgcacgtg
      181 gtgaaagagg cattaccggg ccacaggggc caaaaggtga aacaggagca cgtggcccgg
      241 caggaccaca gggaccaaaa ggtgaaacag gtgaacgcgg tccggcaggt ccacagggag
      301 caaagggcga tacaggagca cgcggcccgg caggtccaca gggaccaaaa ggtgaaacag
      361 gtgaacgcgg tccggaaggt ccacagggac caaaaggtga aacaggtgaa cgcggcccgg
      421 caggtccaca gggagcaaag ggcgaaacag gt
//
LOCUS       DBBGLQ010000496          452 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.503, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000496 DBBGLQ010000000
VERSION     DBBGLQ010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.503"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..209)
                     /locus_tag="ACIYSB_005914"
     CDS             complement(<1..209)
                     /locus_tag="ACIYSB_005914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432660.1"
                     /translation="MSETRFHGARVRESTGLTTAINDVDSSVIGIVATADDADETLFP
                     LNKPILLTRVSDVLGKTGTKGTLYRS"
BASE COUNT          110 a          139 c          100 g          103 t
ORIGIN      
        1 gaacggtaca gcgtgccctt tgttcccgtc ttacccagca catcactgac gcgggtcagc
       61 agtatgggtt tgttcagcgg gaacagagtt tcatccgcgt catctgccgt agccacaata
      121 ccaataacac tggaatccac atcattaatg gcggtggtaa ggccggtact ttcccttacg
      181 cgcgcaccat gaaagcgtgt ttcactcata tgtttcaccc ttgtctggct ggttgatttt
      241 gcgtaatcat tacccacgat gccgccggta tcatctcctg cccgttctca ccgttcgctt
      301 acaacaaaca gccgttcacc acggtcgcac gccgtgaaaa catgacggga cacaccacga
      361 gggcagacca tgaccgacct gttaatcaac gttccgtcac cggatatcga aatcaccatc
      421 aacaacaaca gactccgtaa cgtcacggag cg
//
LOCUS       DBBGLQ010000497          451 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.504, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000497 DBBGLQ010000000
VERSION     DBBGLQ010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.504"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT          124 a          103 c           89 g          135 t
ORIGIN      
        1 tcatgcaact cccgcattaa aatatactgt tctttccgga taattacgtt aaaatataca
       61 cctgcgtgca tctgtgtcag actccgttag ataccagcgt acattgtaaa catgccggat
      121 gcacgcaata caataatcgg tcttttcgga gttatagtca ccaggctggc atcccgtaaa
      181 tatgtatcag gtagcaattc aggaagcgaa caaaagcgcc tggagaaggt tatctctgat
      241 aataatttac atgaaatgtc tcctttattc ttcgcgaata aggccttcag tgttctcccc
      301 ttttcagcaa ttcttaccgt agtaatcact cctgcctgtg tataacacga gaattgccag
      361 agtttaacgt ttcggtatcg ttatcctgca tgatgttgaa cagaaccggg cgtgaattct
      421 gcgccagaat tttctttcga ggaacattcc g
//
LOCUS       DBBGLQ010000498          446 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.505, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000498 DBBGLQ010000000
VERSION     DBBGLQ010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.505"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(114..>446)
                     /locus_tag="ACIYSB_005915"
     CDS             complement(114..>446)
                     /locus_tag="ACIYSB_005915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGC5432661.1"
                     /translation="VEPAPLYYLWSREAMANDTTPYLMVLLLNNDVWCKSREVVVLRN
                     LIEQAWGKVLDEDNSITHQVVYPEVLSLVFDAANTYNAEPGVLEQTAQAYIESHGAIE
                     RHLGCSDF"
BASE COUNT          126 a          120 c           92 g          108 t
ORIGIN      
        1 cgttaaaacc gggaactacg ccacaacaac gacatcctgt ggtcgggctc gacttattta
       61 attgtgtaaa cagggcctct cagcgaaagg cccttatgca tcacctgacg tcgttaaaag
      121 tcactacatc caagatgacg ttcaatggca ccatgcgatt caatgtaagc ctgggccgtc
      181 tgttccagta caccaggctc agcgttatat gtgttagctg catcaaatac caacgacagc
      241 acttcaggat acacaacctg atgtgtaatg gagttatctt cgtccaatac ttttccccac
      301 gcctgctcaa tcagatttct gagaaccacc acctcacgac tcttacacca gacgtcgtta
      361 ttaagtagca gtaccataag ataaggagtg gtatcgttag ccatggcctc cctactccag
      421 agataatata aaggggcggg ctcaac
//
LOCUS       DBBGLQ010000499          445 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.506, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000499 DBBGLQ010000000
VERSION     DBBGLQ010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.506"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            16..445
                     /locus_tag="ACIYSB_005916"
                     /pseudo
     CDS             16..445
                     /locus_tag="ACIYSB_005916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT           97 a          124 c          121 g          103 t
ORIGIN      
        1 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
       61 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
      121 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
      181 atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt
      241 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
      301 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg
      361 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg aagacggttg
      421 ttgcgcacgt attcggtgaa cgcac
//
LOCUS       DBBGLQ010000500          444 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.507, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000500 DBBGLQ010000000
VERSION     DBBGLQ010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.507"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>444
                     /gene="rfaY"
                     /locus_tag="ACIYSB_005917"
     CDS             <1..>444
                     /gene="rfaY"
                     /locus_tag="ACIYSB_005917"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HGC5432662.1"
                     /translation="LVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSI
                     NDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVS
                     GDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNE"
BASE COUNT          165 a           62 c           80 g          137 t
ORIGIN      
        1 cttgtactta aagtttatgc gccaaagcat aaaatgaccg aacgtttctt aaaatcttgc
       61 ataaaaaaag attattatga aaatttaatt tatcaaaccg atcgggtacg tggtgaaggc
      121 atccaatcaa ttaatgacta ttttttgcta gctgaacgta aaacattaaa tttcgctcat
      181 tattatatta tgctcattga gtatattgaa ggtgtcgggt taaatgagta tttggaaatt
      241 tctgaagatc tgaaggatca attatcagaa tcgataaaag agttgcatca gcatggaatg
      301 gtgtctggcg atcctcataa aggaaatttt atagtatccg aaaaaggttt aagactaatt
      361 gacctttcag gtaaaaaaac aaccgctgtt ttaaaagcaa aagatcgcat tgatcttgaa
      421 cggcactata atataaaaaa tgaa
//
LOCUS       DBBGLQ010000501          437 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.508, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000501 DBBGLQ010000000
VERSION     DBBGLQ010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.508"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>437)
                     /locus_tag="ACIYSB_005918"
     CDS             complement(<1..>437)
                     /locus_tag="ACIYSB_005918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGC5432663.1"
                     /translation="HIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGC
                     GWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKV
                     QNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDG"
BASE COUNT          104 a          128 c          121 g           84 t
ORIGIN      
        1 tcaccatctt tcagtttgaa gcaaaccgcc gggatgcctt cgtccgggcg acccgtacag
       61 atgaactcat acggccccag tttggcgatt tcatccgcca gataagcggc aacctggtaa
      121 gaggcgttct gtactttggt atagccttca cgaccgaggc gcaggaattc atagtactgt
      181 gcaattacct gacccgccgg gcgggagaag ttgatggcaa aagtaccaat ttgaccaccc
      241 aggtagtcaa cgttgaacac cagttcctgc ggcagcgctt cttcgtcacg ccagataacc
      301 cagccgcagc ccagcggagc cagaccgaat ttatggcctg aagcactgat cgatttcaca
      361 cgcggcaggc ggaagtccca gacgatatcc ggggcgacga acggtgccag gaagccaccg
      421 ctggcagcgt cgatgtg
//
LOCUS       DBBGLQ010000502          435 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.509, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000502 DBBGLQ010000000
VERSION     DBBGLQ010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.509"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(13..258)
                     /locus_tag="ACIYSB_005919"
     CDS             complement(13..258)
                     /locus_tag="ACIYSB_005919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432664.1"
                     /translation="MSWMKLTEAERKRINDAYAAQAAQLKLSGRDELPREVKRKVRVK
                     VLRMIRAERKARTAKAQRTKAYRAAENTFTWQPARRR"
BASE COUNT           88 a          122 c           86 g          139 t
ORIGIN      
        1 ccgctggccg gctcacctgc gtcgcgccgg ctgccaggta aacgtgttct ctgcggcacg
       61 atacgctttt gttcgttgag cctttgcggt tctcgctttg cgctctgccc gaatcattct
      121 caaaactttt acacgcactt ttctcttaac ttcccgtggc agttcatccc tgccggacaa
      181 ctttaactgt gcagcctgtg cggcataagc atcgttgata cgcttacgtt ctgcttccgt
      241 cagtttcatc caagacataa ttcctcctgg tgacttatcc gtgttatgtc tttaatctgt
      301 atccccttcg ttgctatgcc tccgggatat caggtattac ctttcgaaga attttataaa
      361 aaaccacctg tgcagaggga aatagtggat ttgcccatat atttccagac atctgttatc
      421 acttaaccca ttaca
//
LOCUS       DBBGLQ010000503          428 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.510, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000503 DBBGLQ010000000
VERSION     DBBGLQ010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.510"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>428)
                     /locus_tag="ACIYSB_005920"
     CDS             complement(<1..>428)
                     /locus_tag="ACIYSB_005920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5432665.1"
                     /translation="TVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVMLNLLVFIR
                     SSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQAVAILTE
                     LQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQ"
BASE COUNT          100 a          100 c          114 g          114 t
ORIGIN      
        1 tcctgggttg tgtcataccc catcaccctc agggctttat ttacagtatt ttcactgatt
       61 ggtttacgcg ggtcatgtgc tcccgtaaag atcagaccat tttcaccagc ccatgtctgt
      121 agttctgtca gtatggctac agcctgtttg ctgagtggca caagatgtgg tgttcgcatc
      181 ttggagcccc ggtgtgagaa ttttacgcct ggcaggggtt cgcgttcggc tggaatagtc
      241 cacatggcat tgtcaatatc aatttcagac cagcgggcgt atctgagttc actggaacga
      301 ataaaaacca gtaaattcag cataacggca agctgggtga caggttgccc cttgtaactg
      361 ccaatacgcg acaaaagttc aggcaggcga gaaagatcaa gagcggggcg atgctggcgt
      421 tttaccgt
//
LOCUS       DBBGLQ010000504          428 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.511, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000504 DBBGLQ010000000
VERSION     DBBGLQ010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.511"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>428)
                     /locus_tag="ACIYSB_005921"
     CDS             complement(<1..>428)
                     /locus_tag="ACIYSB_005921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGC5432666.1"
                     /translation="SVKRQHRPALDLSRLPELLSRINSYKGQPVTRLAVMLNLLVFIR
                     SSELRYARWAEIDIDNSMWTIPAERKPLPGVKFSHRGSKMRTPHLVPLSKQAVAILTE
                     LQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTK"
BASE COUNT          105 a           96 c          109 g          118 t
ORIGIN      
        1 tccttggttg tgtcataccc catcactctg agggccttat ttacggtatt ttcactgatt
       61 ggtttacgcg ggtcatgtgc tcccgtaaag atcagaccat tttcaccagc ccatgtctga
      121 agttctgtca gtatggctac agcctgtttg ctgagtggca caagatgtgg tgttcgcatc
      181 ttggagcccc ggtgtgagaa ttttacccct ggcagaggtt tgcgttcggc tggaatagtc
      241 cacattgaat tgtcaatatc aatttcagcc cagcgggcat atctgagttc actggagcga
      301 ataaaaacca gtaaattcag cataacagca agccgggtga caggctgacc tttataactg
      361 ttaatacgcg acagaagttc aggcagacgt gaaagatcaa gagcggggcg atgctgtcgt
      421 tttacaga
//
LOCUS       DBBGLQ010000505          424 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.512, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000505 DBBGLQ010000000
VERSION     DBBGLQ010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.512"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..150)
                     /locus_tag="ACIYSB_005922"
     CDS             complement(<1..150)
                     /locus_tag="ACIYSB_005922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432667.1"
                     /translation="MGEGSALPVGVPVPWPSATPPTGWLKCNGAAFSSEKYPNLAKAY
                     PTLKLP"
     gene            complement(161..>424)
                     /locus_tag="ACIYSB_005923"
     CDS             complement(161..>424)
                     /locus_tag="ACIYSB_005923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGC5432668.1"
                     /translation="FIRGWDDGRGIDAGRTLLSWQDGTSFSHYGGNFDIGSGHSIINY
                     DQIVSNQPGFSRFSFAGPSRGDGVNYVTIRPRNIAFNYIVRAA"
BASE COUNT          125 a          110 c           93 g           96 t
ORIGIN      
        1 aggtaatttt aacgttgggt aagcctttgc cagatttggg tacttttcag aagaaaatgc
       61 tgcaccattg catttcagcc accccgttgg cggcgtggct gagggccacg gaacaggcac
      121 accaacgggc agcgcagagc cttcccccaa accaacgttt ttatgccgcc cttacgatat
      181 aattaaacgc aatgttacga ggacgaatgg ttacataatt aaccccatca cctcgtgaag
      241 gccctgcaaa tgaaaaacgg gaaaatcctg gttggttaga aacaatttgg tcatagttaa
      301 tgattgaatg acctgaccca atgtcgaaat tacctccgta atgagaaaaa cttgtaccat
      361 cctgccatga aagcagggta cgtcccgcat caatcccgcg cccatcatcc cagccacgga
      421 taaa
//
LOCUS       DBBGLQ010000506          421 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.513, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000506 DBBGLQ010000000
VERSION     DBBGLQ010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.513"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>421)
                     /gene="wzx"
                     /locus_tag="ACIYSB_005924"
     CDS             complement(<1..>421)
                     /gene="wzx"
                     /locus_tag="ACIYSB_005924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033815058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O166 family O-antigen flippase"
                     /protein_id="HGC5432669.1"
                     /translation="KLYFIILVFLCEGLISRTLGVSDYGKWIYAINTIIIISSIALVA
                     GSEVMVPALSKHKKIVGELLSSAFIIRMLGALTSFFFINIYIFFAIKDEVLQQMLMSL
                     SLILLFNEPFGVITNYCQSRINMKGIVLARCVSLGVRT"
BASE COUNT          158 a           82 c           56 g          125 t
ORIGIN      
        1 tgttctcact cctaaggata cacatcttgc caacacaata cctttcatat taattcgtga
       61 ttgacaataa tttgtaataa cgccaaacgg ttcattaaac aaaagaatta gtgacaagga
      121 catcaacatt tgttgtaaaa cttcatcctt gatcgcgaaa aaaatatata tgttaataaa
      181 aaaaaatgaa gttagtgccc ctaacattct aatgataaat gcagaactta acaactctcc
      241 gactattttt ttatgtttac ttaaggcagg gaccataact tcactccctg caactaaagc
      301 gatcgaagat attatgataa tggtgttaat tgcgtaaatc cacttcccat aatcactaac
      361 gcctaaagtt ctgctaatta agccctcaca taagaaaacg agaatgatga aatataactt
      421 t
//
LOCUS       DBBGLQ010000507          419 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.514, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000507 DBBGLQ010000000
VERSION     DBBGLQ010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.514"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..321
                     /gene="yjdP"
                     /locus_tag="ACIYSB_005925"
     CDS             <1..321
                     /gene="yjdP"
                     /locus_tag="ACIYSB_005925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGC5432670.1"
                     /translation="FPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDRGRD
                     YEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERRMED
                     EYGR"
BASE COUNT          101 a          114 c          125 g           79 t
ORIGIN      
        1 tttccgctat ttcttttatt caccctgctc acgctgtcca ccgttccggc ccaggccgat
       61 attatcgacg acaccattgg caatattcag caggcgatta atgacgctta taaccctgac
      121 cgtgggcgag actatgaaga ttcgcgcgac gacggctggc agcgtgaagt gagcgacgat
      181 cggcgcaggc aatatgacga ccgccgccgc cagtttgaag atcgccgcag gcagctggac
      241 gatcgccagc gccagcttga tcaggagcgt cggcaactgg aggatgaaga gcggagaatg
      301 gaagatgagt atgggcgatg aggttgggta tggagcaggc atcgcccctc accccaatgg
      361 ggagagggag aaaacgagcg caatattcaa tatcgagcac aatcggtccc ctcgcccct
//
LOCUS       DBBGLQ010000508          409 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.515, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000508 DBBGLQ010000000
VERSION     DBBGLQ010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.515"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT          105 a          129 c          102 g           73 t
ORIGIN      
        1 gacagatatg cacctcccac cgcaagcggc gggccccgac cggagccatt ttagttacaa
       61 cactcaaata caaccgccaa gaaaaccccg gtccagcgca gaaccgaaac cacaaagccc
      121 ctccctcata actgaaaagc ggccccgccc cggcccaaag ggccggaaca gagtcgcttt
      181 taattatgaa tgttgtaact acttcatcat cgctgtcagt cttctcgctg gaagttctca
      241 gtacacgctc gtaagcggcc ctgacggccc gctaacgcgg agatacgccc cgacttcggg
      301 taaaccctcg tcgggaccac tccgaccgcg cacagaagct ctgtcatggc tgaaaacggg
      361 tatggcttag cagggctggg gatgggtaag gtgaaatcta tcagtcagt
//
LOCUS       DBBGLQ010000509          403 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.516, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000509 DBBGLQ010000000
VERSION     DBBGLQ010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.516"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>403
                     /locus_tag="ACIYSB_005926"
     CDS             <1..>403
                     /locus_tag="ACIYSB_005926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGC5432671.1"
                     /translation="EFAAKMQDATGTASEDMMGLFDTIQKAFYLGVDDTNMLSFFTKT
                     SSVLKMVNKDGLQAAQSLAPISVMMDQMGMNGESAGNALRKVIQSGLSVKKIRDVNKI
                     MARQKLGVQLDFTDGKGSFGGLDNMFRQLAKL"
BASE COUNT          110 a           87 c          114 g           92 t
ORIGIN      
        1 gagtttgccg caaagatgca ggatgctacc ggaacggcct cagaagacat gatggggctg
       61 ttcgacacaa ttcagaaggc gttttatctg ggcgttgacg acaccaacat gctgtcattc
      121 ttcactaaaa ccagctctgt tctgaagatg gtgaataagg acggtcttca ggctgcacag
      181 agtcttgccc ccatcagtgt catgatggat cagatgggga tgaacgggga gtcggcaggt
      241 aatgccctgc gaaaagttat ccagtccgga ttaagcgtta agaaaatcag ggacgtcaat
      301 aaaatcatgg cccgccagaa actcggggta cagctcgatt ttactgatgg caaagggagt
      361 tttggcggtc ttgataacat gttcaggcaa ctggcaaagc tgc
//
LOCUS       DBBGLQ010000510          398 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.517, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000510 DBBGLQ010000000
VERSION     DBBGLQ010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.517"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>398
                     /locus_tag="ACIYSB_005927"
     CDS             <1..>398
                     /locus_tag="ACIYSB_005927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGC5432672.1"
                     /translation="DNMDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKR
                     VYRIMRQNALLLERKTAVPPSKRAHTGKVAVKESNQRCCSDGFEFRCDNGEKLRVTFA
                     LDCCDREALYWAVTTGGFDSETVQDVMPGAV"
BASE COUNT           95 a           97 c          120 g           86 t
ORIGIN      
        1 gataatatgg atgtacttct ccgtatacac catgttatcg gagagctgcc cacgtatggt
       61 tatcgtcggg tatgggcgct gcttcgcaga caggccgaac ttgatggtat gcctgcgatc
      121 aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc tgttgcttga gcgaaaaacc
      181 gctgtaccgc catcgaagcg ggcacatact ggcaaagtgg ccgtgaaaga aagcaatcaa
      241 cgatgctgct ctgacgggtt cgagttccgc tgtgataacg gagaaaaact gcgagtcacg
      301 ttcgcgctgg actgctgtga ccgtgaggca ctgtactggg cggtcactac gggcggcttc
      361 gacagtgaaa cagtacagga cgtcatgcca ggagcggt
//
LOCUS       DBBGLQ010000511          394 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.518, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000511 DBBGLQ010000000
VERSION     DBBGLQ010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.518"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..394
                     /locus_tag="ACIYSB_005928"
                     /pseudo
     CDS             1..394
                     /locus_tag="ACIYSB_005928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    98..184
                     /locus_tag="ACIYSB_005928"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          101 a           98 c          112 g           83 t
ORIGIN      
        1 atggtgggct caccaccgct gaacgtcagc gtctgaaaga gctggaacgc gaaaatcgtg
       61 aactgcgccg cagtaacgat atccttcgcc aggcttccgc ttattttgcg aaggcggagt
      121 tcgaccgcct ctggaaaaaa taatgccact gctggataag ctgcgtgagc tgcagtgagc
      181 tgcatattgc cccgtcaacg tattaccact gtcagcaaca gcagcatcat cctgataaac
      241 gcagcgcccg tgcgcagcgt gatgactggc tgaagaaaga gatacagcgc gtatacgatg
      301 aaaatcacca ggtgtacggt gtgcgtaaag tctggcgtca gttgttacgg gaaggtatca
      361 gagtggccag atgcactgtg acacgtctta tggc
//
LOCUS       DBBGLQ010000512          393 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.519, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000512 DBBGLQ010000000
VERSION     DBBGLQ010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.519"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>393
                     /locus_tag="ACIYSB_005929"
     CDS             <1..>393
                     /locus_tag="ACIYSB_005929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HGC5432673.1"
                     /translation="GQDATTGRKGIVQLSSATNSNSETLAATPKAVSDALSMAFVKPT
                     TSLGETDLNTLGRKEDAGDYYQQSDSGARTDRNYPVEKAGELRIRVGAWGFCQHEYTS
                     WDPPRKFIRTVTGNFNGNGPWSEWVEVT"
BASE COUNT          120 a           92 c          119 g           62 t
ORIGIN      
        1 aggacaggat gcaaccaccg gacgcaaagg catcgtacag ctgagcagcg ccacaaacag
       61 caattccgaa acgctggcgg ccacaccgaa ggcagtaagt gatgcactga gcatggcgtt
      121 cgtgaagcca acaacatccc tgggggaaac agacctgaac actctggggc gtaaggaaga
      181 cgcgggagat tattaccagc agagtgactc aggtgcgaga acagacagaa actatcctgt
      241 agagaaggca ggagagttac gcatccgtgt tggtgcatgg ggattctgcc agcatgaata
      301 cacgagctgg gatccgccac gaaaatttat caggacagtt accggaaact ttaacggtaa
      361 cggcccatgg tcagagtggg tggaggtgac aaa
//
LOCUS       DBBGLQ010000513          393 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.520, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000513 DBBGLQ010000000
VERSION     DBBGLQ010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.520"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            7..393
                     /locus_tag="ACIYSB_005930"
                     /pseudo
     CDS             7..393
                     /locus_tag="ACIYSB_005930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          145 a           59 c           58 g          131 t
ORIGIN      
        1 taatgaaccg ttaaaaaacc gttaaaaaac caggaaatat ccacaggtaa tatcaatatt
       61 aattgcccat taaaagatat ttacaatgaa atcaggaggt taaacgtaat ttttagttgt
      121 gggactggag atatcgttga tctatcctct ctggacttac gtaatgtcga tttagattat
      181 tatgatttca cagataaaca tatggctaat actattttaa atccttttaa attgaattca
      241 acaaatttta ctaatgccaa catgtttcag gttaattttg ttagttcaac acaaaacgcc
      301 acaatctcct gggattattt actaaaaata acgcctgttt taataagcat tagcgatatg
      361 tattctgaag aaaaaatcaa gtttgtcgaa agt
//
LOCUS       DBBGLQ010000514          387 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.521, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000514 DBBGLQ010000000
VERSION     DBBGLQ010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.521"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            1..367
                     /locus_tag="ACIYSB_005931"
                     /pseudo
     CDS             1..367
                     /locus_tag="ACIYSB_005931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           99 a           94 c           90 g          104 t
ORIGIN      
        1 caatggagtt ttgtcggcag taaagcgcga cagcactggc tctggtacac gtacaacacg
       61 aaaactggtg gcgttctggc ctacactttt ggcctacgga ctgattaaac ctgtcgtgaa
      121 ttgctggcgc tgcttgccct tttaccatcg gtatgttgac cactgacgaa tggggtagtt
      181 acaccagaga gttaccgaag gagaagcatc tgactggtaa gatttttact cagcgcattg
      241 agcgcaacat ctacctctga gaacccgaat caaacgtctg gcccgcagaa ctatctgttt
      301 ctcgcgctct gtagaacttc atgaaaaagt catcggtgcc tttatcgaaa aatatatgtt
      361 ctactaattg gagtcaccac cttcgtt
//
LOCUS       DBBGLQ010000515          386 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.522, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000515 DBBGLQ010000000
VERSION     DBBGLQ010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.522"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>386
                     /locus_tag="ACIYSB_005932"
     CDS             <1..>386
                     /locus_tag="ACIYSB_005932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HGC5432674.1"
                     /translation="GWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTGHLMLTT
                     IHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRRPWHEVATERTD
                     DERRLVESVCNPDQVYLRNHEGCPHC"
BASE COUNT           86 a          114 c          111 g           75 t
ORIGIN      
        1 ggctgggtgg actccattaa atcagcactg cgtcttgacc ctgacgggat cctgaacggc
       61 gaaatccgtg accacgattc cgccattacg gcaataaaag ccgccatgac cgggcatctg
      121 atgctgacca ccatccatgc gaacgacccg attaacatcc tcgaacgtct tgagatggag
      181 ggggtacagg ccaggatgat tgccgacccg caactgttca ttggcctgct gagccagcgt
      241 ctggttcagc tgatatgtcc gcactgccgt cgcccctggc atgaggtggc taccgagcgc
      301 acggatgatg aacggcgcct ggtggaaagc gtctgcaacc cggaccaggt atatctgcga
      361 aatcatgaag gctgcccgca ctgctg
//
LOCUS       DBBGLQ010000516          381 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.523, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000516 DBBGLQ010000000
VERSION     DBBGLQ010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.523"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>381)
                     /locus_tag="ACIYSB_005933"
     CDS             complement(<1..>381)
                     /locus_tag="ACIYSB_005933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HGC5432675.1"
                     /translation="FERKDAKNFTHWHGIAAGRLDEAIVSKFFKGEKDDVETVDVILR
                     PKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSREGFLYPTPATSIPRDLLEPLPKGAF
                     SIGEIGQYDKYKTTHTTFSINFDDS"
BASE COUNT           98 a           94 c           87 g          102 t
ORIGIN      
        1 gctgtcatca aagttgatag agaacgtggt atgggtcgtc ttgtatttgt catactgccc
       61 aatctcacca atcgaaaatg ctccttttgg caaaggttca agcaggtctc tgggaatgga
      121 ggtcgctggc gtcggatata aaaaaccctc acggcttagc aaggctggcg tcactatcgg
      181 ggtaacaata tcaggcgcac ctgcagaacg gtccttacca tgctgcagta accggaaata
      241 aacttttggg cgcaagatga catcgaccgt ttcgacatcg tctttttctc ccttaaaaaa
      301 tttactgaca atcgcttcgt caagacgtcc cgccgctatc ccatgccaat gagtgaaatt
      361 tttggcgtct ttccgttcaa a
//
LOCUS       DBBGLQ010000517          375 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.524, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000517 DBBGLQ010000000
VERSION     DBBGLQ010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.524"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..185)
                     /locus_tag="ACIYSB_005934"
     CDS             complement(<1..185)
                     /locus_tag="ACIYSB_005934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HGC5432676.1"
                     /translation="MSTVKVWGLGDAVVDLLPEKPGTLLQCPGGAPANVAVGVARLGG
                     LSGFVGCVGDDPFGRFMR"
BASE COUNT           97 a          131 c           85 g           62 t
ORIGIN      
        1 cgcataaaac gcccgaaagg gtcatccccc acacacccga caaacccgct caggcccccc
       61 agtcgggcca cgcccacggc aacattggca ggcgcaccac cagggcactg caacagcgtg
      121 cccggcttct ccggtaataa atccaccacg gcatcaccga gaccccatac ttttactgta
      181 ctcattcata ctccatgatt ttcatcagtc atattcagcg gatgacagac agctcaaaca
      241 tcgccatatc cgtggttccg gctggaattg gataccaacc cgcattccgg agcagggcag
      301 cgagggcaga cacatcgccg gtcaggggat gcagcaacct cccctgataa tcaaatgccg
      361 cctcaagacg tgagc
//
LOCUS       DBBGLQ010000518          375 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.525, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000518 DBBGLQ010000000
VERSION     DBBGLQ010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.525"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>375
                     /locus_tag="ACIYSB_005935"
     CDS             <1..>375
                     /locus_tag="ACIYSB_005935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5432677.1"
                     /translation="IETANAWLPTFIEAYNNRFATSPRTTDNAHLDVHHSEEELGYIF
                     SLQAKRVLSKNLTFQYKSSAFQVRSEGRGYRLRHSVVTVCENFDGEINVLYDGKALGW
                     EKYVDGPEPIPLDDEKSVHERV"
BASE COUNT          100 a           77 c          100 g           98 t
ORIGIN      
        1 tattgaaaca gcaaatgcat ggttgccgac ctttattgaa gcctataaca accggttcgc
       61 tacgtcgcct cgtactactg ataatgctca tcttgatgtg caccattctg aagaggaact
      121 gggttatatc ttcagcctac aggcgaagcg cgttctgtct aaaaatctca ctttccagta
      181 caaaagcagt gcgtttcagg tacgcagtga gggccgggga tatcgactta ggcattcggt
      241 tgttactgta tgcgagaact ttgacggtga aattaacgtt ctgtatgacg ggaaagcgct
      301 gggctgggaa aagtatgttg atggcccgga gcctatacca ctggatgatg aaaagagtgt
      361 ccatgaacga gtgga
//
LOCUS       DBBGLQ010000519          368 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.526, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000519 DBBGLQ010000000
VERSION     DBBGLQ010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.526"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT           86 a           99 c           78 g          105 t
ORIGIN      
        1 tgccattaat attacctgct gattgcttca catccggatt cctcgcactg cgcgcgggtt
       61 attgtttcgt tttcggccac ggggtcgcca gggctctgtt tgcaggccat gatggcctga
      121 cctgtactgt ttctcaggca ctgagatgtc cgtttacgcc atttgttccc ggatatcctt
      181 aacctctgac gtttgttccc tgcccctctg tctgataacg aatacgctca caacgcacag
      241 tactgaacaa taccgacaac aacatgtctt acatgaaatt cgctgtaaca tccaccggaa
      301 aacattttca gatatcggat gaggtactac ttccattgac agcgcgaggt aacaaaattg
      361 atggtaac
//
LOCUS       DBBGLQ010000520          364 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.527, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000520 DBBGLQ010000000
VERSION     DBBGLQ010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.527"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            153..>364
                     /locus_tag="ACIYSB_005936"
     CDS             153..>364
                     /locus_tag="ACIYSB_005936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HGC5432678.1"
                     /translation="MPLTVRQIESAKPADKDYKLSDGQGLYLLVKKSGARYWNLKYRF
                     AGKEKKLVIGVYPDISLAQARQKRDEA"
BASE COUNT           97 a           84 c           81 g          102 t
ORIGIN      
        1 aactgatttc aacagagcta acccctcccc ctttaacccc ctttgaaata agatatcatc
       61 agaaattcct tttctctggg ggtcaatcat attccatgaa catcaagatg attttggggg
      121 ctattctggg ggctacattt caggggtaac tcatgccact tactgttcga caaattgagt
      181 cggctaaacc tgctgacaag gattacaaac tttctgacgg tcagggcctt tatcttcttg
      241 tcaaaaaatc cggtgcccgt tactggaatc tgaagtaccg gtttgccggt aaggaaaaga
      301 aactggtgat tggtgtctat cctgacatat cactggctca ggctcgtcag aagcgggatg
      361 aagc
//
LOCUS       DBBGLQ010000521          361 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.528, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000521 DBBGLQ010000000
VERSION     DBBGLQ010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.528"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>361
                     /locus_tag="ACIYSB_005937"
     CDS             <1..>361
                     /locus_tag="ACIYSB_005937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432679.1"
                     /translation="NASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNF
                     VEQVQKMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRN
                     VVASTLRTTAISEAAYAV"
BASE COUNT           83 a          100 c          106 g           72 t
ORIGIN      
        1 aatgccagca caatgcttgg gtatgtttct gatgcgatga aagtggtgga ttctgccgta
       61 tcggatgccg ccaggctgtt gcagggggat atctcggtac ttctgccgcc gccatcgtca
      121 ggcaaaaatt tcgttgagca ggtgcagaaa atgtggcgta ccgggaaacg cctttatggt
      181 aacgccagcg acctggtcac catgatcaaa acgctttccg gtgtcagcct cggcagcgat
      241 ctgcaaccgc gcggcgtctg gaaaacggac agtaaaacca ccgccacggc gacgcagcag
      301 cgtaacgtgg ttgccagcac ccttcgtacg accgcaatca gcgaagcggc gtatgccgtc
      361 a
//
LOCUS       DBBGLQ010000522          361 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.529, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000522 DBBGLQ010000000
VERSION     DBBGLQ010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.529"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..299
                     /locus_tag="ACIYSB_005938"
     CDS             <1..299
                     /locus_tag="ACIYSB_005938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432680.1"
                     /translation="NSAPIILITMSIDESHIYAVIRFIAIVIYCLSLVRKSRSLKLGE
                     TLNAIIGSTEGDMCRQQKESFAALTQHKACIDAFLCGCTVICARTGTSLPILQQ"
BASE COUNT           96 a           74 c           85 g          106 t
ORIGIN      
        1 ataattctgc cccgattata ctaatcacga tgagtatcga tgagtctcat atttatgctg
       61 ttattagatt cattgccata gttatttatt gcttgtcgct ggttcgtaag agcaggagct
      121 taaaactcgg ggagacgctg aatgccataa ttggcagtac agaaggtgat atgtgtcgtc
      181 aacaaaagga gtcgttcgct gccctgacgc aacataaagc gtgcattgat gcttttctct
      241 gtggttgtac agttatatgc gcaaggacgg gaaccagtct gccgatcctg cagcaataac
      301 gggcaatctc cgtacgccct tattgattac acctgtgtgt aagttcacag agatattgca
      361 a
//
LOCUS       DBBGLQ010000523          360 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.530, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000523 DBBGLQ010000000
VERSION     DBBGLQ010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.530"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            122..197
                     /locus_tag="ACIYSB_005939"
     tRNA            122..197
                     /locus_tag="ACIYSB_005939"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
BASE COUNT          101 a           80 c           83 g           96 t
ORIGIN      
        1 tgaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacctc aatatctcaa
      241 aacagactgt taagtcttgt ttgatatatt tgctctttaa aaatctggat caagctgaaa
      301 attgaaacac tgaacaatga aagttgttcg tgagtctctc aaattttcgc aactctgaag
//
LOCUS       DBBGLQ010000524          359 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.531, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000524 DBBGLQ010000000
VERSION     DBBGLQ010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.531"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..63)
                     /locus_tag="ACIYSB_005940"
     CDS             complement(<1..63)
                     /locus_tag="ACIYSB_005940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432681.1"
                     /translation="MAISSRNTLLAALAFIAFQAQ"
BASE COUNT          124 a           63 c           56 g          116 t
ORIGIN      
        1 ctgtgcctga aaagcgatga atgccagtgc ggcaagaagt gtgttacgcg atgaaattgc
       61 catgattgtc tgctcccctg tcttgttatg ggagcagtat tcaggaataa aaacattcat
      121 taaaagaatt agtcgttatc gcacagatga ttttattctt agcaaaaaaa cggctaagga
      181 aagattaaat atgcaattac tccgttctaa caattgggaa aattactaca cttcatttta
      241 gtgatgcata aacaaccaca ttacgagatt tattacttca ttatttctat caatatcaat
      301 acatataata aaactattat attcgcattg tatatatcac tgtatcattc tcaatcact
//
LOCUS       DBBGLQ010000525          358 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.532, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000525 DBBGLQ010000000
VERSION     DBBGLQ010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.532"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            24..>358
                     /locus_tag="ACIYSB_005941"
     CDS             24..>358
                     /locus_tag="ACIYSB_005941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5432682.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKLSVEAALNAEMT
                     HHLGYEKNQSRPGANSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTRITG
                     MDNQILSLYA"
BASE COUNT          112 a           94 c           80 g           72 t
ORIGIN      
        1 cagcatcaaa tgaaggttaa tttatggacg aaaaacagtt acaggctctg gctaacgaac
       61 tggccaaaaa cctcaaaacc cctgaagacc tcagtcagtt tgatcggctg ctgaaaaagc
      121 tcagcgttga agccgctctc aatgcagaga tgacacacca tcttgggtat gagaaaaatc
      181 agtccagacc aggagctaac tcccgcaacg gttattccac aaagaccgtt atcacaggcg
      241 acggtccact ggaactgcgt actccgcgcg atcgtgacgg taccttcgaa ccacaactgg
      301 taaagaaaaa tcagacccgt attaccggga tggataacca gatcctctcg ttgtatgc
//
LOCUS       DBBGLQ010000526          356 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.533, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000526 DBBGLQ010000000
VERSION     DBBGLQ010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.533"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>356
                     /locus_tag="ACIYSB_005942"
     CDS             <1..>356
                     /locus_tag="ACIYSB_005942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGC5432683.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DBBGLQ010000527          353 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.534, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000527 DBBGLQ010000000
VERSION     DBBGLQ010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.534"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT           99 a           81 c           77 g           96 t
ORIGIN      
        1 attacactgt gggttgacgc cggaagaatc agagaacaga taccgttctt actgtaaaac
       61 gtggctaata ttaattgagc actgcagtgt tcagagcaca aagaagaaaa aagatgtgtg
      121 cgaatgcctg taatgtgtta aaagaatgtt ccgcgtttcc ggtacgcttt tcgtaactaa
      181 caatgcaggg tgacgtaacg ctggttgtgc cggaaacgcg tccctccccc tatctttcct
      241 gtttggattt gcccctatat ttctggatta tccgacaaaa ccggcgtgaa gtattactct
      301 cgtcgcccag caccataagg ataacgccca taaaatcatg aaacttttct tcc
//
LOCUS       DBBGLQ010000528          352 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.535, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000528 DBBGLQ010000000
VERSION     DBBGLQ010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.535"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>352
                     /locus_tag="ACIYSB_005943"
     CDS             <1..>352
                     /locus_tag="ACIYSB_005943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HGC5432684.1"
                     /translation="CSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQ
                     DVMLGAVERRFGSELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNG
                     IAESFVKTIKRDYIS"
BASE COUNT           86 a           78 c          119 g           69 t
ORIGIN      
        1 gtgctctgac gggtttgagt tccgctgtga taacggagaa aaactgcggg tcacgttcgc
       61 gctggactgc tgtgaccgtg aggcactgca ctgggcggtc acaacgggtg gcttcgacag
      121 tgaaacagta caggacgtca tgctgggagc agtggaacgc cgctttggca gcgagcttcc
      181 ggcgtctcca gtggagtggc tgacggataa tggttcatgc taccgggcga atgaaacacg
      241 tcagttcgcc aggatgttgg gacttgaacc gaagaacacg gcagtgcgga gtccggagag
      301 taacggaata gcagagagct tcgtgaaaac gataaagcgt gactacataa gt
//
LOCUS       DBBGLQ010000529          348 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.536, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000529 DBBGLQ010000000
VERSION     DBBGLQ010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.536"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..344)
                     /locus_tag="ACIYSB_005944"
     CDS             complement(<1..344)
                     /locus_tag="ACIYSB_005944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 32"
                     /protein_id="HGC5432685.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLC"
BASE COUNT           93 a           87 c           81 g           87 t
ORIGIN      
        1 tagcaaagtg tgagaacgcc ctgattatta accgcgcttc ctgagtaaca accattacgg
       61 tcgtactctt tatcgggcat tagcgctaat ggttcatgct gccagtgcaa taaatccgtc
      121 gaactccagt gcccccaaca tttgtactta tgatcacaag ccagtggatt ccactgataa
      181 aacagatgat agcggcctcc agaccagcaa aagccatttg gatcgttcat tagtccggtg
      241 acgggagcga gatgccaaca aggatagtgg gcatctgcca gtgctttgga ctgacctttc
      301 atcactgcct gtaaaatcgc gggcatttgt gaaggcatcg acattact
//
LOCUS       DBBGLQ010000530          346 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.537, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000530 DBBGLQ010000000
VERSION     DBBGLQ010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.537"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>346)
                     /locus_tag="ACIYSB_005945"
     CDS             complement(<1..>346)
                     /locus_tag="ACIYSB_005945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432686.1"
                     /translation="TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHI
                     HFIGYLDGQRHKNSYQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKS
                     AVDDLRYALSYLA"
BASE COUNT           92 a           88 c           73 g           93 t
ORIGIN      
        1 agccagatag ctaagggcgt aacgtaagtc gtctaccgcg gacttatcac tatagtgaat
       61 cacgtgatcg atacgcaccg ggtatttatc tttagcccgg cacagatgaa aataaccctc
      121 cccttccgtg atccgcctcc agatatcccc cagttgcctg gatatctgat aagagttctt
      181 gtgacgttgt ccatccagat agccgataaa atgaatatga agccctttgt ctggtgtata
      241 ttccataacc cagtaatatc ctgccaacat cgtctgggtt tcgctaagta aacggtatat
      301 ttccatacac atactgtgct tacaggaatg cccgaaactg ggcgtg
//
LOCUS       DBBGLQ010000531          340 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.539, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000531 DBBGLQ010000000
VERSION     DBBGLQ010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.539"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>340
                     /locus_tag="ACIYSB_005946"
     CDS             <1..>340
                     /locus_tag="ACIYSB_005946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HGC5432687.1"
                     /translation="NRVVVYMGDDQKFEYIYKFVSEGKFNPQDRKANMHLLEKGTLYV
                     AKFNDDGKGEWLPLVFGQNGLDASKGFENQGDLLIKTRLAADAVGATKMDRPEWIAVD
                     PYHAGSVYCTL"
BASE COUNT           94 a           83 c           97 g           66 t
ORIGIN      
        1 aaccgcgttg tggtctatat gggcgatgac cagaaattcg aatacatata taaatttgtc
       61 tcggaaggaa aattcaaccc gcaggatcgc aaagccaaca tgcacctgct ggaaaaaggc
      121 actctgtacg tggcgaaatt taacgacgac ggcaaaggcg agtggctacc gctggtgttc
      181 ggacaaaatg ggctcgacgc cagtaaaggg tttgaaaacc agggcgacct gttgattaaa
      241 acccgtctgg cggcagacgc cgtgggggcc actaaaatgg accgcccgga gtggattgcg
      301 gtcgatccat atcacgccgg cagcgtttac tgcacgctca
//
LOCUS       DBBGLQ010000532          339 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.540, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000532 DBBGLQ010000000
VERSION     DBBGLQ010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.540"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(<1..>339)
                     /locus_tag="ACIYSB_005947"
     CDS             complement(<1..>339)
                     /locus_tag="ACIYSB_005947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunoglobulin-binding protein"
                     /protein_id="HGC5432688.1"
                     /translation="ALLLSAQAQAQAQAILIGPIQPGGHSSFLVGDSVAGRSSGDIRN
                     VWLVGDNSFLLDSNGSVLLGNNSGVVSSPGSVSLGHDALIADSEWGTVAGKAASLISS
                     RQSSAIGAFS"
BASE COUNT          110 a           99 c           64 g           66 t
ORIGIN      
        1 atgaaaacgc accaattgcc gagctctgac gagaagaaat aagtgacgct gcttttcctg
       61 caacagtccc ccattcagag tcagcaataa gtgcatcatg ccccagactc acacttcccg
      121 gagagctcac aacaccagaa ttattcccca aaagcacact accgtttgaa tctaataaaa
      181 aagaattatc acccaccaac catacatttc ttatgtcacc agacgaacgg ccagctactg
      241 aatccccaac aagaaagcta ctatgtccgc caggttgaat cggtccgatt aaaatagcct
      301 gagcctgagc ctgagcctga gctgagagca gcagggcca
//
LOCUS       DBBGLQ010000533          339 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.541, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000533 DBBGLQ010000000
VERSION     DBBGLQ010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.541"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT          128 a           56 c           57 g           98 t
ORIGIN      
        1 ttaatgagat taatattggc aaccattata gcctccttcc cacggtttaa gagcaaacat
       61 acaacataat tattcataac atacagagca gtaaatgaat gatactgaca atgatgctag
      121 tccattatct ttgcataata tttgttagct atagatttta aaaatattta tgagaaaagt
      181 atggcaccag agggggtttt gactatcaaa aatcgctcaa acatgaacta agatgatgaa
      241 ttaagcatta tctgcgataa ttgccactca gttctacaaa taatatttaa atgctcctga
      301 acagtgatgt agcaagacac gtatggagcc aacgaagaa
//
LOCUS       DBBGLQ010000534          332 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.542, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000534 DBBGLQ010000000
VERSION     DBBGLQ010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.542"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..276
                     /locus_tag="ACIYSB_005948"
     CDS             <1..276
                     /locus_tag="ACIYSB_005948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGC5432689.1"
                     /translation="GIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMNGNRAEMSPEA
                     AGIAACLMAYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT           79 a           89 c           94 g           70 t
ORIGIN      
        1 ggcatctgga atctgtacac cctgaacaac ggtggcgcat ttatggcacc tgaaccggat
       61 gacgatgatg acgaaacatg ggtgctgttt aacgtcatga acggtaaccg cgctgaaatg
      121 agcccggaag ccgccggcat tgccgcctgt ctgatggcgt acagccatca cgcctgtcgt
      181 acagagaatt atgccatgac ggttcattat taccggctgc gggattatgc cctgcagcat
      241 ccggaatgca gcgccattat gcgcatcatc gactgaatgg aggcagggac aatgcagcag
      301 ctttcctttc tgcccggaga gatgacaccc gg
//
LOCUS       DBBGLQ010000535          330 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.543, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000535 DBBGLQ010000000
VERSION     DBBGLQ010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.543"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>330
                     /locus_tag="ACIYSB_005949"
     CDS             <1..>330
                     /locus_tag="ACIYSB_005949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGC5432690.1"
                     /translation="DNRGFEADRVTLTIDDADGQLQLPQRGARLNVMIGWRGEALVNK
                     GVYIVDEVTWEGPPDKLTITASSADFREEFNVKREVSWHDVTVKQVVSAIAHRYGLKA
                     QISDILMD"
BASE COUNT           76 a           92 c          106 g           56 t
ORIGIN      
        1 gacaaccggg gctttgaggc ggaccgcgtc accctgacca ttgacgacgc ggacgggcag
       61 cttcagctgc cacagcgcgg cgcacgcctg aacgtgatga ttggctggcg cggggaggcg
      121 ctggttaaca agggtgtcta catcgttgat gaagtgacgt gggaaggccc gccggacaaa
      181 ctgaccatca ccgccagcag cgccgatttt cgcgaagagt tcaacgtgaa gcgtgaagtc
      241 tcctggcacg acgtgacggt aaaacaggtt gtgtcagcca tcgcgcaccg ctacggcctg
      301 aaagcacaga taagcgatat cctcatggat
//
LOCUS       DBBGLQ010000536          327 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.544, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000536 DBBGLQ010000000
VERSION     DBBGLQ010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.544"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..>327
                     /locus_tag="ACIYSB_005950"
     CDS             <1..>327
                     /locus_tag="ACIYSB_005950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5432691.1"
                     /translation="KNQTRITGMDNQILALYARGMTTREITSAFKEMYDADVSPTLIS
                     KVTDAVKEQVSEWQNRPLDALYPIVYLDCIVVKVRHSGSVINKAVFLALGINTDGQKE
                     LLDMWL"
BASE COUNT           87 a           80 c           86 g           74 t
ORIGIN      
        1 agaaaaatca gacccgcatt accggcatgg acaaccagat cctggcgctg tatgccagag
       61 ggatgactac ccgcgaaatt acctcagcct tcaaagaaat gtacgacgcc gatgtctcgc
      121 ccacgctgat atccaaagtg accgatgcgg ttaaagaaca ggtctctgaa tggcaaaacc
      181 gaccgttgga tgcactgtat cccattgttt atcttgactg tattgtggtt aaggttcgtc
      241 acagcgggag tgtcattaac aaagcggtgt tcctcgcgct gggcatcaat accgacggtc
      301 agaaagagct gctggatatg tggctgg
//
LOCUS       DBBGLQ010000537          326 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.545, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000537 DBBGLQ010000000
VERSION     DBBGLQ010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.545"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(233..>326)
                     /locus_tag="ACIYSB_005951"
     CDS             complement(233..>326)
                     /locus_tag="ACIYSB_005951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432692.1"
                     /translation="ASGQVTQWIKREFSLQGILTGIYQDLMHRA"
BASE COUNT           84 a           82 c           55 g          105 t
ORIGIN      
        1 aacggtgatg aatattatcc aaatctgatg gttcgtagcg ttttcagccc ccagattctg
       61 agttgcctgt agggatgatt tcaattcccg atttgtctat cgcatagaga aaaagaattt
      121 catttctgtt caccagaacc catgtaaccc actccaattc gttatttatc tcactgattg
      181 cccttcattt atctcattga tgcaccgaaa ggtaaaactg ccatccggtg catcacgctc
      241 gatgcatcaa atcctgataa atccccgtca gtattccttg taagctaaat tctcttttta
      301 tccactgagt gacttgaccg cttgcc
//
LOCUS       DBBGLQ010000538          326 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.546, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000538 DBBGLQ010000000
VERSION     DBBGLQ010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.546"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
BASE COUNT          104 a           46 c           64 g          112 t
ORIGIN      
        1 ctgaaaagat atttttgtct gccagaaaaa aaatcctcat acatgatgag gattaaatct
       61 taaaggtcca ttctatggag tgtcggattg aaaaattcta cgggtgatat ctttcacatt
      121 ttaaatatta tttcaaccat cgtttccttg tggtttttat cacataaaaa ccacaaggaa
      181 attcaggggg cttatatgtg tggctgaata caggaagtga ctgggggcag ccagatatca
      241 ttatgtgata tttacagtga atttgatttt atatctggca ctggaaatca aaagtcagag
      301 tattattctt ttcatgttga tatgat
//
LOCUS       DBBGLQ010000539          308 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.547, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000539 DBBGLQ010000000
VERSION     DBBGLQ010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.547"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            121..>308
                     /locus_tag="ACIYSB_005952"
     CDS             121..>308
                     /locus_tag="ACIYSB_005952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432693.1"
                     /translation="MNAIPYFDYSMAPFWPSYQNKIVGVLNRALREQSCSRVRRILLR
                     LPWEHDNAFSCRKTWLGM"
BASE COUNT           64 a           90 c           72 g           82 t
ORIGIN      
        1 ccatccccgg ttccacggtt atcacatcaa caacctcagt acacgacgcc ttcagtttca
       61 ccatacgctc gtctttcgct ggcggggatg cgtcgtgtgt ttccatatgg agcatatctt
      121 atgaatgcta tcccttattt cgactatagc atggcaccct tctggccatc ttatcagaac
      181 aaaatcgtcg gcgttctcaa tcgtgcgctg cgtgaacagt cctgctcccg ggtacggcgg
      241 atcctgcttc gtctgccgtg ggaacatgac aacgccttca gttgcagaaa gacctggtta
      301 ggcatgga
//
LOCUS       DBBGLQ010000540          303 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.denovo.548, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000540 DBBGLQ010000000
VERSION     DBBGLQ010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.denovo.548"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            46..>303
                     /locus_tag="ACIYSB_005953"
     CDS             46..>303
                     /locus_tag="ACIYSB_005953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGC5432694.1"
                     /translation="MDEKKLKVLAAELAKGLKTEADLNQFSRMLTKLTVETALNAELT
                     DHLGHEKNAPKSGSNTRNGYSSKTVLCDDGEIELNTPRDREN"
BASE COUNT           96 a           75 c           68 g           64 t
ORIGIN      
        1 gcctgttttt gatatgttca ctccaacaac ggagacaggc aaacgatgga cgaaaagaaa
       61 cttaaagtcc ttgcggctga actggccaaa ggccttaaaa ccgaagccga cctcaaccag
      121 ttttcacgca tgttgacaaa gcttaccgtc gaaacggcgc tcaatgcaga actgactgac
      181 catctaggtc atgagaaaaa tgctcctaaa tcaggctcta atacccgtaa tggctactcg
      241 tcaaaaacgg tattgtgcga tgatggcgag atcgaactta acacgccgcg tgaccgtgaa
      301 aat
//
LOCUS       DBBGLQ010000541         2690 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.guided.268, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000541 DBBGLQ010000000
VERSION     DBBGLQ010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.guided.268"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            107..565
                     /gene="gpt"
                     /locus_tag="ACIYSB_005954"
     CDS             107..565
                     /gene="gpt"
                     /locus_tag="ACIYSB_005954"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGC5432695.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            657..1901
                     /gene="frsA"
                     /locus_tag="ACIYSB_005955"
     CDS             657..1901
                     /gene="frsA"
                     /locus_tag="ACIYSB_005955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGC5432696.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEDDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            1959..2360
                     /gene="crl"
                     /locus_tag="ACIYSB_005956"
     CDS             1959..2360
                     /gene="crl"
                     /locus_tag="ACIYSB_005956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGC5432697.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(2399..>2690)
                     /locus_tag="ACIYSB_005957"
     CDS             complement(2399..>2690)
                     /locus_tag="ACIYSB_005957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGC5432698.1"
                     /translation="PITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGD
                     EDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT          655 a          654 c          697 g          684 t
ORIGIN      
        1 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
       61 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
      121 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
      181 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt
      241 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
      301 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
      361 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
      421 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
      481 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
      541 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
      601 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
      661 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
      721 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
      781 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
      841 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
      901 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
      961 ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga agacgatccg caactcagtg
     1021 gtcgtcactg gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag
     1081 gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg cgcctatgaa gaggccgctc
     1141 agcgtctacc gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca
     1201 ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacggtatta atgtgtggtg
     1261 gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg
     1321 gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca
     1381 cccaggactc cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg
     1441 tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc
     1501 tggcatacct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata
     1561 ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg
     1621 ccagtcgttt ggggatgcat gatgcttccg atgaagcgtt gcgcgtggag ctgaatcgct
     1681 attcattaaa agtgcaagga ttgctgggac gtcgctgccc aacgccaatg ttatcaggct
     1741 actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg
     1801 ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt gacaaaggtc
     1861 ttcaggaaat caccgactgg atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc
     1921 aatttggtaa aacagttgca tcacaacagg agatagcaat gacgttaccg agtggacacc
     1981 cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt
     2041 gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac
     2101 cggaagtgcg tgaattctgg ggctggtgga tggagctgga agcgcaggaa tcccgtttta
     2161 cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt gttccggtaa
     2221 aagacactga agtggttgaa cgactggagc acaccctgcg tgagtttcac gagaagctgc
     2281 gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt cgtgacgagc
     2341 cggtgaagtt aacggcgtga gtgaaatgtg ccggatgcat cacatccggc aatattcatt
     2401 aaaactgata cgtcatgcca accgcgacaa tatcatcatt attaatattc aatttgttat
     2461 cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg ttgaaataat
     2521 acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct tcaatatctt
     2581 tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag
     2641 cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
//
LOCUS       DBBGLQ010000542         2382 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.guided.280, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000542 DBBGLQ010000000
VERSION     DBBGLQ010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.guided.280"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            68..871
                     /locus_tag="ACIYSB_005958"
                     /note="aph(3'')-Ib"
     CDS             68..871
                     /locus_tag="ACIYSB_005958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HGC5432699.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            871..1659
                     /locus_tag="ACIYSB_005959"
     CDS             871..1659
                     /locus_tag="ACIYSB_005959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HGC5432700.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGGQFGYRKLLYVKAVF"
     gene            complement(1633..>2382)
                     /locus_tag="ACIYSB_005960"
     CDS             complement(1633..>2382)
                     /locus_tag="ACIYSB_005960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HGC5432701.1"
                     /translation="HYTDTAGFTDHVFALMHLLGFAFCPRIRDLHDKKLFIKGKAEKY
                     PALQSLISTTSLNLKEIEIHWREVLRLATSIKQGTVTASLMLKKLASYPKQNGLAKAL
                     REIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARNALARAVFMHRLGEIRDRKPENQ
                     SYRASGLTLLTAAISLWNTVYMERAVDALKRKGVKINEQLLSHLSPLGWEHINLTGDY
                     IWKSNRIPASGKFRRLRPAKVERSKNSLNVQ"
BASE COUNT          483 a          631 c          662 g          606 t
ORIGIN      
        1 gtcttagcgt gggatatttt ccgttttcca agcggacccc ttcatcagaa aactgaagga
       61 acctccattg aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt
      121 cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa
      181 aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct
      241 caaaggtcga ggtgtggctt gccccgaggt gatcaactgg caggaggaac aggagggtgc
      301 atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt
      361 gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga
      421 tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc
      481 ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga
      541 tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgatat
      601 ggttgtttgc catggtgatc cctgcatgcc gaacttcatg gtggacccta aaactcttca
      661 atgcacgggt ctgatcgacc ttgggcggct cggaacagca gatcgctatg ccgatttggc
      721 actcatgatt gctaacgccg aagagaactg ggcagcgcca gatgaagcag agcgcgcctt
      781 cgctgtccta ttcaatgtat tggggatcga agcccccgac cgcgaacgcc ttgccttcta
      841 tctgcgattg gaccctctga cttggggttg atgttcatgc cgcctgtttt tcctgctcat
      901 tggcacgttt cgcaacctgt tctcattgcg gacacctttt ccagcctcgt ttggaaagtt
      961 tcattgccag acgggactcc tgcaatcgtc aagggattga aacctataga agacattgct
     1021 gatgaactgc gcggggccga ctatctggta tggcgcaatg ggaggggagc agtccggttg
     1081 ctcggtcgtg agaacaatct gatgttgctc gaatatgccg gggagcgaat gctctctcac
     1141 atcgttgccg agcacggcga ctaccaggcg accgaaattg cagcggaact aatggcgaag
     1201 ctgtatgccg catctgagga acccctgcct tctgcccttc tcccgatccg ggatcgcttt
     1261 gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc
     1321 cacgcggcga ttatagccga tcaaatgatg agcaatgcct cggaactgcg tgggctacat
     1381 ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg gctggctggt gatagatccc
     1441 gtcggtctgg tcggtgaagt gggctttggc gccgccaata tgttctacga tccggctgac
     1501 agagacgacc tttgtctcga tcctagacgc attgcacaga tggcggacgc attctctcgt
     1561 gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg gggtcagttt
     1621 ggatatcgaa aattattgta cgttaaggct gttttttgac ctttcgactt tagctggtct
     1681 tagccgacga aacttaccag aagccggtat ccggttgctt ttccagatat aatcacctgt
     1741 cagattgatg tgttcccagc ccaacggcga caaatgcgac agcagttgct cgttgatctt
     1801 caccccttta cgcttcaggg catcgaccgc tctttccata tagacggtat tccacaggga
     1861 tatggcagcc gtcagcagcg tcagtccact ggcgcgatag ctctgatttt ccggttttct
     1921 gtccctgatt tctcccagtc ggtgcataaa taccgcacgc gccagcgcgt tacgggcttc
     1981 gcctttattc agccccgcct gtacccgccg acgcagagcc gggtcgcgga accagtcgag
     2041 cataaacagg gttcgctcaa tgcggccaat ttctctcagt gctttggcaa gtccgttttg
     2101 cttgggatag ctggcaagct ttttcagcat cagggatgcc gtcacggttc cctgctttat
     2161 cgaggttgcc agacgcagca cttcacgcca gtggatttcg atctctttca gattcaggct
     2221 ggtcgttgat atcagtgact gaagcgccgg gtatttctct gctttccctt tgataaacag
     2281 ttttttgtcg tggagatccc tgattcgcgg acagaacgca aatcccaaca gatgcatcag
     2341 ggcaaagaca tgatcggtga aaccagcggt atcggtgtaa tg
//
LOCUS       DBBGLQ010000543         2142 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.guided.291, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000543 DBBGLQ010000000
VERSION     DBBGLQ010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.guided.291"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            40..939
                     /gene="aac(3)-VIa"
                     /locus_tag="ACIYSB_005961"
     CDS             40..939
                     /gene="aac(3)-VIa"
                     /locus_tag="ACIYSB_005961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HGC5432702.1"
                     /translation="MTDPRKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPH
                     VSLRAVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSW
                     PAFDPDHAPAYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYG
                     PRSPIARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWV
                     TTSDWDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFG
                     IEWLEARHAAPAAAALKPKQRRD"
     gene            1179..1466
                     /gene="groES"
                     /locus_tag="ACIYSB_005962"
     CDS             1179..1466
                     /gene="groES"
                     /locus_tag="ACIYSB_005962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009684394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGC5432703.1"
                     /translation="MNIKPLYDRVVIKRTEEEKVSAGGIVIPDSAAEKPIKGQVVAVG
                     EGKVFDNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIFAVLG"
     gene            1522..>2142
                     /locus_tag="ACIYSB_005963"
     CDS             1522..>2142
                     /locus_tag="ACIYSB_005963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HGC5432704.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELADKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAVAAGMNPMDLKRGIDKAVTSAVEELKKISKPCSTSKEIAQVGSISANSDTDIGE
                     LIAKAMDKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINN"
BASE COUNT          421 a          712 c          677 g          332 t
ORIGIN      
        1 tgcataataa gccctacaca aattgggaga tatatcatga tgactgatcc ccgcaaaaac
       61 ggcgatttgc acgaacccgc gacggcaccc gcgacgccct ggtccaaaag cgagctggtc
      121 cggcaattgc gcgacctcgg cgtgcgctca ggcgatatgg tgatgccgca tgtgtcgttg
      181 cgcgccgtcg ggccgctggc ggacggaccg cagacacttg tcgatgcgct gatcgaggcc
      241 gtcggcccca ccgggaatat tctcgccttc gtctcgtggc gcgattcgcc ctatgaacag
      301 acgctgggtc atgatgcgcc gcccgccgcc atcgcccaaa gctggcctgc gttcgacccc
      361 gaccatgcgc ccgcctaccc cggctttggc gcgatcaacg aatttatccg aacctatccg
      421 gggtgtcggc gcagcgccca tcccgacgca tcgatggcgg cgatcgggcc cgatgcggcg
      481 tggctggtgg cgccgcacga gatgggcgcc gcttatggcc cccgctcgcc gatcgcgcgt
      541 tttctcgccc acgcaggaaa aatcctgtcg atcggcgccg ggcccgatgc agtcaccgcg
      601 ctccattatg ccgaagcggt ggcgcggatc gagggcaagc gccgcgtcac ttattcgatg
      661 cccttactgc gcgaaggcaa gcgcgtctgg gtcaccacgt ccgactggga ttcgaacggc
      721 atcctcgacg aatatgccgc gcccgacggc cccgacgcgg tcgaacggat cgcccgcgac
      781 tatctcgccc gcaccagggt tgcgcaaggc ccggtcggcg gcgcgcaatc ccggctgatc
      841 gacgcggccg atatcgtttc cttcggcatc gaatggctcg aggcgcgcca cgccgcgcca
      901 gcggcggcag cgctgaagcc gaaacaacgc cgcgactgag tcgtcgatta ttccggacat
      961 atattcctcc ctctcgccca gccgcatagc atcaggccca caagcccgcc gcctcttcaa
     1021 caaggatgtc gggacgttct tcagtaaaga acagcttggc accctcgaca aggatgattg
     1081 cccgaggcga tcaggcggcg aatttttgcg cgcgcgcacg gtcgcggcgg cgcgcgataa
     1141 aggcgatggg tagccccacg aggaagatat gcgcggcgat taacatcaag ccgctgtacg
     1201 accgcgtcgt catcaagcgc accgaggaag agaaggtctc cgccggtggt atcgtgatcc
     1261 cggattcggc cgccgagaag ccgatcaagg gccaggtcgt cgccgtgggc gagggcaagg
     1321 tgttcgacaa cggcaacgtg cgcgcgccga aggtcaaggt cggcgaccag gtgctgttcg
     1381 gcaagtacgc cggcaccgag gtcaagctcg acggcaccga gtacctggtg gtgaaggaag
     1441 acgacatctt cgccgtgctg ggctgagccc gccgcttcgc cgcatcccaa cccgcaatca
     1501 cagaactgag gtaattccgc aatggctgcc aaggatattc gtttcggtga agacgcccgc
     1561 gcccgcatgg tccgcggcgt gaacgtgctc gccaacgccg tcaaggcgac cctgggcccg
     1621 aagggccgca acgtcgtgct cgagaagagc ttcggcgccc cgacgatcac caaggacggc
     1681 gtgtccgtcg ccaaggagat cgaactggcc gacaagttcg agaacatggg cgcgcagatg
     1741 gtcaaggaag tcgcttccaa gacctccgac aacgccggcg acggcaccac caccgccacc
     1801 gtgctggccc aggccctgat ccgcgagggc atgaaggccg tggccgccgg catgaacccg
     1861 atggacctca agcgcggcat cgacaaggcc gtcacctcgg ccgtcgagga gctgaagaag
     1921 atctccaagc cctgctcgac cagcaaggag atcgcccagg tcggttcgat ctcggccaac
     1981 tccgacaccg acatcggcga gctgatcgcc aaggccatgg acaaggtcgg caaggaaggc
     2041 gtgatcaccg tcgaggaggg ctcgggcctg gagaacgagc tcgacgtcgt cgagggcatg
     2101 cagttcgacc gcggctacct gtcgccgtac ttcatcaaca ac
//
LOCUS       DBBGLQ010000544         2109 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.guided.293, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000544 DBBGLQ010000000
VERSION     DBBGLQ010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.guided.293"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            complement(65..1078)
                     /gene="intI1"
                     /locus_tag="ACIYSB_005964"
     CDS             complement(65..1078)
                     /gene="intI1"
                     /locus_tag="ACIYSB_005964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HGC5432705.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1224..2062
                     /gene="aadA1"
                     /locus_tag="ACIYSB_005965"
                     /pseudo
     CDS             1224..2062
                     /gene="aadA1"
                     /locus_tag="ACIYSB_005965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          483 a          588 c          609 g          429 t
ORIGIN      
        1 tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat tgacttgcgc
       61 tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc actccggcac
      121 cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa tggccgagca
      181 gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg aacgagtggc
      241 ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca cgtttgaagg
      301 cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc cgtggatcgg
      361 tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg gcgcgcggat
      421 acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc tggtccttca
      481 gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag ctctcgggta
      541 acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg ccgtgatcga
      601 aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc gttccataca
      661 gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga accacttcat
      721 ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc tcctgaagcc
      781 agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc aatgcctgac
      841 gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt
      901 cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
      961 agttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca
     1021 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     1081 cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1141 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1201 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     1261 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1321 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     1381 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1441 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     1501 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1561 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     1621 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     1681 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     1741 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     1801 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     1861 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     1921 gacaagaaga agatcgcttg gcctcccgcg cagatcagtt ggaagaattt gttcactacg
     1981 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     2041 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     2101 gccaaacta
//
LOCUS       DBBGLQ010000545         1907 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.guided.307, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000545 DBBGLQ010000000
VERSION     DBBGLQ010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.guided.307"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..78
                     /locus_tag="ACIYSB_005966"
     CDS             <1..78
                     /locus_tag="ACIYSB_005966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HGC5432706.1"
                     /translation="EPIIKSLWAVTVVATIVILFVALYW"
     gene            89..448
                     /gene="frdD"
                     /locus_tag="ACIYSB_005967"
     CDS             89..448
                     /gene="frdD"
                     /locus_tag="ACIYSB_005967"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGC5432707.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            511..1644
                     /locus_tag="ACIYSB_005968"
     CDS             511..1644
                     /locus_tag="ACIYSB_005968"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGC5432708.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ"
     gene            1733..>1907
                     /gene="blc"
                     /locus_tag="ACIYSB_005969"
     CDS             1733..>1907
                     /gene="blc"
                     /locus_tag="ACIYSB_005969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGC5432709.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLE"
BASE COUNT          455 a          489 c          487 g          476 t
ORIGIN      
        1 gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt aatcctgttt
       61 gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc gttctgacga
      121 accggtattc tggggcctct tcggggccgg tggtatgtgg agcgccatca ttgcgccggt
      181 gatgatcctg ctggtgggta ttctgctgcc actggggttg tttccgggtg atgcgctgag
      241 ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc tgttcctgat
      301 gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc acgatctgaa
      361 aatccacgta cctgcgggca aatgggtttt ctacggtctg gctgctatcc tgacagttgt
      421 cacgctgatt ggtgtcgtta caatctaacg catcgccaat gtaaatccgg cccgcctatg
      481 gcgggccgtt ttgtatggaa accagaccct atgttcaaaa cgacgctctg cgccttatta
      541 attaccgcct cttgctccac atttgctgcc cctcaacaaa tcaacgatat tgtgcatcgc
      601 acaattaccc cgcttataga gcaacaaaag atcccgggta tggcggtggc ggtaatttat
      661 cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc
      721 acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttactgg cgtgcttggt
      781 ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccacaacaaa atactggcct
      841 gaacttaccg ctaaacagtg gaatgggatc acactattac atctcgcaac ctacactgct
      901 ggcggcctgc cattgcaggt gccggatgag gtgaaatcct caagcgactt gctgcgcttc
      961 tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc caactccagt
     1021 atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg
     1081 caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt accgcccgca
     1141 gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt ttcgcctggg
     1201 gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc ccgctgggtg
     1261 caaagcaatt taaaacccct tgatatcaat gagaaaacgc ttcaacaagg gatacaactg
     1321 gcacaatctc gctactggca aaccggcgat atgtatcagg gcctgggctg ggaaatgctg
     1381 gactggccgg taaatcctga cagcatcatt aacggcagtg acaataaaat tgcactggca
     1441 gcacgccccg taaaagcgat tacgccccca actcctgcag tacgcgcatc atgggtacat
     1501 aaaacagggg cgaccggcgg atttggtagc tatgtcgcgt ttattccaga aaaagagctg
     1561 ggtatcgtga tgctggcaaa caaaaactat cccaatccag cgagagtcga cgccgcctgg
     1621 cagattctta acgctctaca gtaaaattcc atcgggtccg aattttcgga ccttttctcc
     1681 gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat ttatgcgcct
     1741 gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgca gttctcctac
     1801 gccgccgcgt ggcgtgaccg tagtaaataa tttcgacgcc aaacgttatc tcggtacctg
     1861 gtatgagatt gcccgttttg atcaccgctt tgaacgtgga ctggaaa
//
LOCUS       DBBGLQ010000546         1864 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.guided.311, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000546 DBBGLQ010000000
VERSION     DBBGLQ010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.guided.311"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            37..384
                     /locus_tag="ACIYSB_005970"
     CDS             37..384
                     /locus_tag="ACIYSB_005970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HGC5432710.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            378..1217
                     /gene="sul1"
                     /locus_tag="ACIYSB_005971"
     CDS             378..1217
                     /gene="sul1"
                     /locus_tag="ACIYSB_005971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HGC5432711.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1345..1845
                     /locus_tag="ACIYSB_005972"
     CDS             1345..1845
                     /locus_tag="ACIYSB_005972"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGC5432712.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
BASE COUNT          342 a          561 c          563 g          398 t
ORIGIN      
        1 tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct ttttcttgtt
       61 atcgcaatag ttggcgaagt aatcgcaaca tccgcattaa aatctagcga gggctttact
      121 aagcttgccc cttccgccgt tgtcataatc ggttatggca tcgcatttta ttttctttct
      181 ctggttctga aatccatccc tgtcggtgtt gcttatgcag tctggtcggg actcggcgtc
      241 gtcataatta cagccattgc ctggttgctt catgggcaaa agcttgatgc gtggggcttt
      301 gtaggtatgg ggctcataat tgctgccttt ttgctcgccc gatccccatc gtggaagtcg
      361 ctgcggaggc cgacgccatg gtgacggtgt tcggcattct gaatctcacc gaggactcct
      421 tcttcgatga gagccggcgg ctagaccccg ccggcgctgt caccgcggcg atcgaaatgc
      481 tgcgagtcgg atcagacgtc gtggatgtcg gaccggccgc cagccatccg gacgcgaggc
      541 ctgtatcgcc ggccgatgag atcagacgta ttgcgccgct cttagacgcc ctgtccgatc
      601 agatgcaccg tgtttcaatc gacagcttcc aaccggaaac ccagcgctat gcgctcaagc
      661 gcggcgtggg ctacctgaac gatatccaag gatttcctga ccctgcgctc tatcccgata
      721 ttgctgaggc ggactgcagg ctggtggtta tgcactcagc gcagcgggat ggcatcgcca
      781 cccgcaccgg tcaccttcga cccgaagacg cgctcgacga gattgtgcgg ttcttcgagg
      841 cgcgggtttc cgccttgcga cggagcgggg tcgctgccga ccggctcatc ctcgatccgg
      901 ggatgggatt tttcttgagc cccgcaccgg aaacatcgct gcacgtgctg tcgaaccttc
      961 aaaagctgaa gtcggcgttg gggcttccgc tattggtctc ggtgtcgcgg aaatccttct
     1021 tgggcgccac cgttggcctt cctgtaaagg atctgggtcc agcgagcctt gcggcggaac
     1081 ttcacgcgat cggcaatggc gctgactacg tccgcaccca cgcgcctgga gatctgcgaa
     1141 gcgcaatcac cttctcggaa accctcgcga aatttcgcag tcgcgacgcc agagaccgag
     1201 ggttagatca tgcctagcat tcaccttccg gccgcccgct agcggaccct ggtcaggttc
     1261 cgcgaaggtg ggcgcagaca tgctgggctc gtcaggatca aactgcacta tgaggcggcg
     1321 gttcataccg cgccagggga gcgaatggac agcgaggagc ctccgaacgt tcgggtcgcc
     1381 tgctcgggtg atatcgacga ggttgtgcgg ctgatgcacg acgctgcggc gtggatgtcc
     1441 gccaagggaa cgcccgcctg ggacgtcgcg cggatcgacc ggacattcgc ggagaccttc
     1501 gtcctgagat ccgagctcct agtcgcgagt tgcagcgacg gcatcgtcgg ctgttgcacc
     1561 ttgtcggccg aggatcccga gttctggccc gacgccctca agggggaggc cgcatatctg
     1621 cacaagctcg cggtgcgacg gacacatgcg ggccggggtg tcagctccgc gctgatcgag
     1681 gcttgccgcc atgccgcgcg aacgcagggg tgcgccaagc tgcggctcga ctgccacccg
     1741 aacctgcgtg gcctatacga gcggctcgga ttcacccacg tcgacacttt caatcccggc
     1801 tgggatccaa ccttcatcgc agaacgccta gaactcgaaa tctaacgtcc gttcgggcat
     1861 cgag
//
LOCUS       DBBGLQ010000547         1259 bp    DNA     linear   BCT 08-NOV-2024
DEFINITION  TPA_asm: Escherichia coli strain E-MBS2716A
            SAMN43563449-rid23703233.guided.374, whole genome shotgun sequence.
ACCESSION   DBBGLQ010000547 DBBGLQ010000000
VERSION     DBBGLQ010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43563449
            Sequence Read Archive: SRR31121283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-OCT-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002430692.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002430692.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 03:34:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,580
            CDSs (with protein)               :: 5,580
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 146 of 306
            Pseudo Genes (incomplete)         :: 173 of 306
            Pseudo Genes (internal stop)      :: 75 of 306
            Pseudo Genes (multiple problems)  :: 74 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43563449-rid23703233.guided.374"
                     /strain="E-MBS2716A"
                     /isolation_source="Abattoir [ENVO:01000925]; Meat (whole
                     or parts) [FOODON:03317170]; Gallus gallus
                     [NCBITaxon:9031]; Carcass [UBERON:0008979]; Food (raw)
                     [FOODON:03311126]; Meat (with bone) [FOODON:02010116];
                     Meat (with skin) [FOODON:02010111]"
                     /host="Chicken [NCBITaxon:9031]"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada: Ontario"
                     /collection_date="2013-03-07"
                     /collected_by="Canadian Food Inspection Agency (CFIA)
                     [GENEPIO,0100552]"
     gene            <1..223
                     /locus_tag="ACIYSB_005973"
     CDS             <1..223
                     /locus_tag="ACIYSB_005973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432713.1"
                     /translation="HEKYHAQKRLNNILKNCLLSAAAQLHRPLSWLCFQMYAILLLQL
                     MDHRKQVTRLGIPFRPEHPYETHARLLLL"
     gene            232..1047
                     /gene="sul2"
                     /locus_tag="ACIYSB_005974"
     CDS             232..1047
                     /gene="sul2"
                     /locus_tag="ACIYSB_005974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HGC5432714.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1191..>1259
                     /locus_tag="ACIYSB_005975"
     CDS             1191..>1259
                     /locus_tag="ACIYSB_005975"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGC5432715.1"
                     /translation="MTAETALRVGMAAGRVFRRGDHR"
BASE COUNT          243 a          373 c          356 g          287 t
ORIGIN      
        1 ccatgaaaaa taccatgctc agaaaaggct taacaatatt ttgaaaaatt gcctactgag
       61 cgctgccgca cagctccata ggccgctttc ctggctttgc ttccagatgt atgctattct
      121 gctcctgcag ctaatggatc accgcaaaca ggttactcgc ctggggattc cctttcgacc
      181 cgagcatccg tatgagactc atgctcgatt attattatta tagaagcccc catgaataaa
      241 tcgctcatca ttttcggcat cgtcaacata acctcggaca gtttctccga tggaggccgg
      301 tatctggcgc cagacgcagc cattgcgcag gcgcgtaagc tgatggccga gggggcagat
      361 gtgatcgacc tcggtccggc atccagcaat cccgacgccg cgcctgtttc gtccgacaca
      421 gaaatcgcgc gtatcgcgcc ggtgctggac gcgctcaagg cagatggcat tcccgtctcg
      481 ctcgacagtt atcaacccgc gacgcaagcc tatgccttgt cgcgtggtgt ggcctatctc
      541 aatgatattc gcggttttcc agacgctgcg ttctatccgc aattggcgaa atcatctgcc
      601 aaactcgtcg ttatgcattc ggtgcaagac gggcaggcag atcggcgcga ggcacccgct
      661 ggcgacatca tggatcacat tgcggcgttc tttgacgcgc gcatcgcggc gctgacgggt
      721 gccggtatca aacgcaaccg ccttgtcctt gatcccggca tggggttttt tctgggggct
      781 gctcccgaaa cctcgctctc ggtgctggcg cggttcgatg aattgcggct gcgcttcgat
      841 ttgccggtgc ttctgtctgt ttcgcgcaaa tcctttctgc gcgcgctcac aggccgtggt
      901 ccgggggatg tcggggccgc gacactcgct gcagagcttg ccgccgccgc aggtggagct
      961 gacttcatcc gcacacacga gccgcgcccc ttgcgcgacg ggctggcggt attggcggcg
     1021 ctgaaagaaa ccgcaagaat tcgttaactg cacattcggg atatttctct atattcgcgg
     1081 ttcagcaggc atgtcccctt tgagggcgac ccgacgacag gataatcgac cttatggtgc
     1141 gcaaatattt cggcacagac ggtattcgtg gcaaagccaa cgaaggcgcg atgacggcgg
     1201 aaaccgcctt gcgcgtcggc atggcggctg gccgtgtctt tcgtcgcggt gaccaccgc
//
